LOCUS       DAZPAC010000001        70301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZPAC010000001 DAZPAC010000000
VERSION     DAZPAC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.001"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..673)
                     /gene="focB"
                     /locus_tag="ACHUPM_000001"
     CDS             complement(<1..673)
                     /gene="focB"
                     /locus_tag="ACHUPM_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFR0867911.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="ACHUPM_000002"
     CDS             complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="ACHUPM_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFR0867912.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="ACHUPM_000003"
     CDS             complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="ACHUPM_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR0867913.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="ACHUPM_000004"
     CDS             complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="ACHUPM_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR0867914.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="ACHUPM_000005"
     CDS             complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="ACHUPM_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR0867915.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="ACHUPM_000006"
     CDS             complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="ACHUPM_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFR0867916.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="ACHUPM_000007"
     CDS             complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="ACHUPM_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR0867917.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="ACHUPM_000008"
     CDS             complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="ACHUPM_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR0867918.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8399..9837)
                     /gene="hyfD"
                     /locus_tag="ACHUPM_000009"
                     /pseudo
     CDS             complement(8399..9837)
                     /gene="hyfD"
                     /locus_tag="ACHUPM_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="ACHUPM_000010"
     CDS             complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="ACHUPM_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR0867919.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="ACHUPM_000011"
     CDS             complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="ACHUPM_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFR0867920.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="ACHUPM_000012"
     CDS             complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="ACHUPM_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR0867921.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="ACHUPM_000013"
     CDS             complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="ACHUPM_000013"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR0867922.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14170..14742)
                     /locus_tag="ACHUPM_000014"
     CDS             complement(14170..14742)
                     /locus_tag="ACHUPM_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR0867923.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14888..15766
                     /gene="dapA"
                     /locus_tag="ACHUPM_000015"
     CDS             14888..15766
                     /gene="dapA"
                     /locus_tag="ACHUPM_000015"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR0867924.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15783..16817
                     /gene="bamC"
                     /locus_tag="ACHUPM_000016"
     CDS             15783..16817
                     /gene="bamC"
                     /locus_tag="ACHUPM_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR0867925.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17030..17743
                     /gene="purC"
                     /locus_tag="ACHUPM_000017"
     CDS             17030..17743
                     /gene="purC"
                     /locus_tag="ACHUPM_000017"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR0867926.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17911..18759
                     /locus_tag="ACHUPM_000018"
     CDS             17911..18759
                     /locus_tag="ACHUPM_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR0867927.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18774..20789
                     /gene="tmcA"
                     /locus_tag="ACHUPM_000019"
     CDS             18774..20789
                     /gene="tmcA"
                     /locus_tag="ACHUPM_000019"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR0867928.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20863..21561
                     /gene="ypfH"
                     /locus_tag="ACHUPM_000020"
     CDS             20863..21561
                     /gene="ypfH"
                     /locus_tag="ACHUPM_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0867929.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21671..21871)
                     /locus_tag="ACHUPM_000021"
     CDS             complement(21671..21871)
                     /locus_tag="ACHUPM_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR0867930.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="ACHUPM_000022"
     CDS             complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="ACHUPM_000022"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR0867931.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23030..23386)
                     /locus_tag="ACHUPM_000023"
     CDS             complement(23030..23386)
                     /locus_tag="ACHUPM_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR0867932.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23768..23827
                     /gene="ypfM"
                     /locus_tag="ACHUPM_000024"
     CDS             23768..23827
                     /gene="ypfM"
                     /locus_tag="ACHUPM_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR0867933.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="ACHUPM_000025"
     CDS             complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="ACHUPM_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR0867934.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="ACHUPM_000026"
     CDS             complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="ACHUPM_000026"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFR0867935.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSPPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29109..31088
                     /gene="aegA"
                     /locus_tag="ACHUPM_000027"
     CDS             29109..31088
                     /gene="aegA"
                     /locus_tag="ACHUPM_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR0867936.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31156..31731
                     /gene="nudK"
                     /locus_tag="ACHUPM_000028"
     CDS             31156..31731
                     /gene="nudK"
                     /locus_tag="ACHUPM_000028"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR0867937.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            31857..32900
                     /locus_tag="ACHUPM_000029"
     CDS             31857..32900
                     /locus_tag="ACHUPM_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR0867938.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="ACHUPM_000030"
     CDS             complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="ACHUPM_000030"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR0867939.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35019..35969)
                     /gene="tal"
                     /locus_tag="ACHUPM_000031"
     CDS             complement(35019..35969)
                     /gene="tal"
                     /locus_tag="ACHUPM_000031"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0867940.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36258..38537
                     /gene="maeB"
                     /locus_tag="ACHUPM_000032"
     CDS             36258..38537
                     /gene="maeB"
                     /locus_tag="ACHUPM_000032"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR0867941.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            38830..39165
                     /gene="eutS"
                     /locus_tag="ACHUPM_000033"
     CDS             38830..39165
                     /gene="eutS"
                     /locus_tag="ACHUPM_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR0867942.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            39178..39657
                     /gene="eutP"
                     /locus_tag="ACHUPM_000034"
     CDS             39178..39657
                     /gene="eutP"
                     /locus_tag="ACHUPM_000034"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR0867943.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            39632..40333
                     /gene="eutQ"
                     /locus_tag="ACHUPM_000035"
     CDS             39632..40333
                     /gene="eutQ"
                     /locus_tag="ACHUPM_000035"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFR0867944.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            40330..41133
                     /gene="eutT"
                     /locus_tag="ACHUPM_000036"
     CDS             40330..41133
                     /gene="eutT"
                     /locus_tag="ACHUPM_000036"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFR0867945.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            41130..42146
                     /gene="pta"
                     /locus_tag="ACHUPM_000037"
     CDS             41130..42146
                     /gene="pta"
                     /locus_tag="ACHUPM_000037"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR0867946.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            42185..42478
                     /gene="eutM"
                     /locus_tag="ACHUPM_000038"
     CDS             42185..42478
                     /gene="eutM"
                     /locus_tag="ACHUPM_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFR0867947.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            42585..42872
                     /gene="eutN"
                     /locus_tag="ACHUPM_000039"
     CDS             42585..42872
                     /gene="eutN"
                     /locus_tag="ACHUPM_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFR0867948.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            42884..44287
                     /locus_tag="ACHUPM_000040"
     CDS             42884..44287
                     /locus_tag="ACHUPM_000040"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFR0867949.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            44298..45134
                     /gene="eutJ"
                     /locus_tag="ACHUPM_000041"
     CDS             44298..45134
                     /gene="eutJ"
                     /locus_tag="ACHUPM_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFR0867950.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            45124..46311
                     /gene="eutG"
                     /locus_tag="ACHUPM_000042"
     CDS             45124..46311
                     /gene="eutG"
                     /locus_tag="ACHUPM_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFR0867951.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            46628..47854
                     /gene="eutH"
                     /locus_tag="ACHUPM_000043"
     CDS             46628..47854
                     /gene="eutH"
                     /locus_tag="ACHUPM_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFR0867952.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            47851..49254
                     /gene="eutA"
                     /locus_tag="ACHUPM_000044"
     CDS             47851..49254
                     /gene="eutA"
                     /locus_tag="ACHUPM_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR0867953.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            49266..50627
                     /gene="eutB"
                     /locus_tag="ACHUPM_000045"
                     /pseudo
     CDS             49266..50627
                     /gene="eutB"
                     /locus_tag="ACHUPM_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            50648..51535
                     /gene="eutC"
                     /locus_tag="ACHUPM_000046"
     CDS             50648..51535
                     /gene="eutC"
                     /locus_tag="ACHUPM_000046"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFR0867954.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            51545..52204
                     /gene="eutL"
                     /locus_tag="ACHUPM_000047"
     CDS             51545..52204
                     /gene="eutL"
                     /locus_tag="ACHUPM_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR0867955.1"
                     /translation="MPALDLIRPSVTAMRVIASVNSEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            52217..52717
                     /gene="eutK"
                     /locus_tag="ACHUPM_000048"
     CDS             52217..52717
                     /gene="eutK"
                     /locus_tag="ACHUPM_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR0867956.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            52763..53815
                     /gene="eutR"
                     /locus_tag="ACHUPM_000049"
     CDS             52763..53815
                     /gene="eutR"
                     /locus_tag="ACHUPM_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFR0867957.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(53821..54720)
                     /gene="hemF"
                     /locus_tag="ACHUPM_000050"
     CDS             complement(53821..54720)
                     /gene="hemF"
                     /locus_tag="ACHUPM_000050"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR0867958.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(54724..55593)
                     /gene="amiA"
                     /locus_tag="ACHUPM_000051"
     CDS             complement(54724..55593)
                     /gene="amiA"
                     /locus_tag="ACHUPM_000051"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR0867959.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTVAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            55807..56232
                     /locus_tag="ACHUPM_000052"
     CDS             55807..56232
                     /locus_tag="ACHUPM_000052"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR0867960.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            56219..56668
                     /locus_tag="ACHUPM_000053"
     CDS             56219..56668
                     /locus_tag="ACHUPM_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR0867961.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            56729..57304
                     /locus_tag="ACHUPM_000054"
     CDS             56729..57304
                     /locus_tag="ACHUPM_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR0867962.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            57400..58299
                     /gene="yfeX"
                     /locus_tag="ACHUPM_000055"
     CDS             57400..58299
                     /gene="yfeX"
                     /locus_tag="ACHUPM_000055"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR0867963.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(58357..59661)
                     /gene="pbp4b"
                     /locus_tag="ACHUPM_000056"
     CDS             complement(58357..59661)
                     /gene="pbp4b"
                     /locus_tag="ACHUPM_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFR0867964.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(59666..61090)
                     /gene="murP"
                     /locus_tag="ACHUPM_000057"
                     /pseudo
     CDS             complement(59666..61090)
                     /gene="murP"
                     /locus_tag="ACHUPM_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(61094..61990)
                     /gene="murQ"
                     /locus_tag="ACHUPM_000058"
     CDS             complement(61094..61990)
                     /gene="murQ"
                     /locus_tag="ACHUPM_000058"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR0867965.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            62154..63011
                     /gene="murR"
                     /locus_tag="ACHUPM_000059"
     CDS             62154..63011
                     /gene="murR"
                     /locus_tag="ACHUPM_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFR0867966.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            63140..63931
                     /gene="ucpA"
                     /locus_tag="ACHUPM_000060"
     CDS             63140..63931
                     /gene="ucpA"
                     /locus_tag="ACHUPM_000060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR0867967.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            64102..65118
                     /gene="cysP"
                     /locus_tag="ACHUPM_000061"
     CDS             64102..65118
                     /gene="cysP"
                     /locus_tag="ACHUPM_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR0867968.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            65118..65951
                     /gene="cysT"
                     /locus_tag="ACHUPM_000062"
     CDS             65118..65951
                     /gene="cysT"
                     /locus_tag="ACHUPM_000062"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR0867969.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            65951..66826
                     /gene="cysW"
                     /locus_tag="ACHUPM_000063"
     CDS             65951..66826
                     /gene="cysW"
                     /locus_tag="ACHUPM_000063"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR0867970.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            66816..67913
                     /gene="cysA"
                     /locus_tag="ACHUPM_000064"
     CDS             66816..67913
                     /gene="cysA"
                     /locus_tag="ACHUPM_000064"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR0867971.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            68047..68958
                     /gene="cysM"
                     /locus_tag="ACHUPM_000065"
     CDS             68047..68958
                     /gene="cysM"
                     /locus_tag="ACHUPM_000065"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR0867972.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(68961..69329)
                     /locus_tag="ACHUPM_000066"
     CDS             complement(68961..69329)
                     /locus_tag="ACHUPM_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR0867973.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            69434..70285
                     /gene="pdxK"
                     /locus_tag="ACHUPM_000067"
                     /gene_synonym="thiJ"
     CDS             69434..70285
                     /gene="pdxK"
                     /locus_tag="ACHUPM_000067"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR0867974.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT        16702 a        18350 c        19432 g        15817 t
ORIGIN      
        1 aatggcgaat ggcaatggcg aacggaatca caaacaaatt ggcgatacag tgctcaaacc
       61 cactggcgac aaacagggtg atgggcaaaa tcatggcgac gattttgtcg cataatgaac
      121 gcccgcaata actcatccac agcgccagac aaaccatcag attgcacata atgccgaggc
      181 tgacagattc agtaaatgta tgatgcattt tgccctcggc gcagtgtaaa accgcgactc
      241 cccacatcgc gttttcactc atcaccagcc cggaaaacca gattaacaaa ctgaaacagg
      301 caatacctgc cagattaccg caggccacca gaagtgcgtt aatcagccaa gttcgccaac
      361 taataacgcc ccgacttttt gccatcaccg tcattaccga cgaggtgaac aggctggtgc
      421 cacaaaccgc cagcaaaata aagccgagtg taaagcaaag gccgcccacc agatgggtta
      481 atgcctgtga agacggggca tcggcgataa cggaaaggta aaaagtaaat ccaatcgcca
      541 taaacacgcc agcggacatc gccattaaaa agaccgacac tttgctgcgg gcaattttat
      601 gggcagtaat ccgttcagcg attgccgctt ttctggcgct caactgcaag tcgaaagaga
      661 gtttgtttct catgcatact cctgcaaaag cagattacaa cacctcgcga accgagagcc
      721 ccagacgctg cattcgtgac agcagcgtgg tgcgcttcat ccctaatcgt gtcgccgcgc
      781 cacggggccc ggcaacaatg ccattggttt ctcgcaatac ctgaacaatg cgctgacgtt
      841 cttcttcgtc attctctggc gtcgtcgggt gcagcaactg cgccattgaa ctgcgaagcg
      901 ctgaatcttc atttagcgtc ggcagtaaat ggctttgtcg gacattaaga tgtaaattca
      961 gactgttacc gcgagtcagc agtaccgctc gctcaatcac gttttccagc tcgcgcacgt
     1021 tgcccggcca gtcccacgac atcaactggc gtagcgcctc ggtcgggatg gcgtcaattg
     1081 aacgattcat atggcgcgcc attttttgcg tgaaatgctt tgctaaaaga gggatatctt
     1141 ccggacggtc gcgtagcggc ggcaattcca gtgggaagac attcaggcga taaaagagat
     1201 cgctgcgaaa ctggcgatct tcaaccattt gccacaaatc acggttggtg gcggcaatga
     1261 ctctgacatt caccgggatc gttctactcc cgccgagacg ctcaatctcc cgctcctgca
     1321 atacgcgcag cagtttaggc tgaagttcta acggcagatc gccaatttca tcaagaaaca
     1381 acgtgccgcc atcggcaatt tcaaaacgac cacgatgggt attaatcgca ccagtaaacg
     1441 cccctttgtc atgaccgaat aactcacttt ccagaagact ggcggggatg gcagcgcagt
     1501 tgattttgac cagcggcttg tcgcgtcgcg ggctaagttg atggatcgct ctggcgatca
     1561 cctctttgcc ggttccggtt tcaccacaaa tcaacactgt actgtcgctc ttcgccacaa
     1621 tatctacctg ctggagcagg tcttccattg cctggctttg atagataatg tcaccgacgc
     1681 ccagattcga aaggagctgc tcgctaagct ggtggttttc ttgctgcaaa ctttcctgca
     1741 aatcggtcat gctacgccag gcatcggcat tgccaacggc aatagcgatg cgatcggcta
     1801 tgtgttgtag tagctgacag ttttcctcac taaagagagt ggaagaggta tgcgccagca
     1861 acaatgcacc cggtgtatgg ttgccaaagg taagcggtat aaggagcgca gattcgcagc
     1921 cgttagatgc aagtaagagt aataacgggt cgcgttgcca gagaaacaga tcgtctgcac
     1981 ggtgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt cagcaataca ctgtcgccag
     2041 gcatattgcg tggcagacac gcacaatgtg aggcagaaag atcgctgcac cacaggctaa
     2101 acttctcgtt ctttcgatga tcgcccagta ccatactgac tgaagccaga ccgaaaaaat
     2161 gatggatctc acgagcgacg tcagcgatca gatcgtcgag atcaagatgt gacagcactg
     2221 cattagtaat atcaaccagt acccgatgat gatcgcgatc tttctccagc aaacgcagcg
     2281 ccacctgctg ttcttcatca ttgagccatg attgcagaac cggggtgatt atccgcgcca
     2341 gccagttaaa cagtgtgcaa tcctggggag taaaacggca gccgtgggtg cgaataaagc
     2401 gtagctccgc cattccacta ctgccgagaa ggacggcgtg acgcgaataa tcgaccatct
     2461 cgccctgata ctctgttgtt gccgtcacat cgctgccatc atcatggtag cggtaataga
     2521 agttgctttt gagaacatgc agttcaataa gttgtaccgg gctgaacggc ggcggcagcg
     2581 ggatgaaggc gcgtaataaa gacgccttac cgtgtttctg cgctaaccgc tccgcgagca
     2641 ttccgtttac cgcttcaatt gttattcctt gcggcggggc aaacatcgcc tcgtctgaca
     2701 tagccatcaa cagtccttat taatgagaaa tccttctcac gcaatttttc tagccatcag
     2761 atagatggcc ggttcctgca caatttcatc caacagatga atgaattgaa tgctacgcgc
     2821 ctgccacaca gacgtcgcgt catccgccag tggcgcgaat gcccgggtca acagggcggt
     2881 gtggctggcg tcaataacaa tctcaccaaa agtcatcacc cccagcatct tacgtcgggc
     2941 ttgctcctct tcgaccagtg caagccaatc ttcgtattcc gtcagtgggc aggggaaggc
     3001 gacattcaga caatcaatca cgccaacgtg atgaccgata gccagcgagt aatacattac
     3061 ctgagagctg tgttccggca tctcgtcgct actggcgaca aacttttttc gcagcgtcca
     3121 gaaaacaatt tccccgcact cttcagtcat gtaacccccg gagtacgcga aagagttgct
     3181 gaacgatact attgagacgt ggatcgtcgg catcgcgcaa ccaggtgtta acccgatttc
     3241 ctgtaggatc gtcgcttaaa tggcgcagga gccgatcgca aatttctcgc ccctgacgat
     3301 agcccgccag ccgccgcgct tctcgctcaa tggcgatacg ctgtgatggc gggatctgcg
     3361 gccacaacgg ttgcatagtc accccggtgg gatcgcgata atccaccgcg tgaatcttct
     3421 gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt ggcggccggt gttggcgggc
     3481 agccggggat ccaaacatca atggggacaa tggtgtcgct gccgccccag acgctgtaga
     3541 gatcgtggaa aataccgccg ccgacaccgc acgcgccgta ggaaacacaa attttatgat
     3601 cgggggcaga ctcatacgcc cgaagtgcag gcatacgcat cgcccgggtg actgcgcctg
     3661 taaataacaa aatatcggcg tgacgcggtg atgaaataac cttaatgcca aaacgttctg
     3721 cgtcgaatac tggtgtgatg gcggcaaaaa tttcgatttc acaggcgttg cagccgccgc
     3781 aatcgacgcg ataaacgtaa gccgagcgac ggatatcctg tagcaaatgc tgcttcatct
     3841 tttgcgtttg ctcgtccagc gtgatgggct ggctgacatg ttgtgtaagc actggactca
     3901 tagctgctcc ttagccacca gcggtacatc tgtatcgtcg ttgagcagcg tcgcgcgttg
     3961 tttgcactcc gggcagacgc tcgcttgcgc ccataacatt tcgcggtttt gtggcgcatt
     4021 ttgttgctgt gccaacaatt cagcagccag tgcgacggtt ttttgcgggg caaacgggcg
     4081 ttcgcaacgg ctgcaacgtt gtagatggaa cgtcgcgcgg gtatagagat cggctttatt
     4141 ggtgacggtc agttcaaagt tattggtaag ctggatggct ctggtcgggc acacttcttc
     4201 acaacgtccg cagtaaatac aacgcctcag atagagctgc caggtgcgcg aattttgctg
     4261 gtcgtcggtc tggatagtca gcgcatttgc cggacaagca caggcgccgc aggcaataca
     4321 ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa
     4381 gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatctt
     4441 ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata
     4501 cggcacggtt tttgactggc gcttgcgcac atctaccagc gtcacacggt cggtacagga
     4561 gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgcag
     4621 catgtaacgc aacaccggcc agttggcgta ggtggcggca cggcagcgcc agcggaacaa
     4681 tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa
     4741 gcccagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg gggtatccgg
     4801 catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt tgacacggac
     4861 catcacgcgg gagaaaacat cgccgccggt aaaggtaaac agtgtttttg gaatattgcc
     4921 gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc cgcggatcag
     4981 cggccctaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt
     5041 gcgttgttcc atattcggcg tggcaagcag catctctacc agctccgaca cgtcggcgcg
     5101 catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat cgcggcgaac
     5161 accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt ccgccattgt
     5221 catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaatc caacaaaatg
     5281 gcaggagagg ccaaggttga gcaaatgact gtgcagacgt tcgacttcca gcagaatcga
     5341 gcgaatagta tgcgctcgtt gcggcacctc aatccccagt gcattttcaa ccgagttggt
     5401 ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cgatccgata agaaggtcac
     5461 ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga cataaaacag
     5521 gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt gacccggttc
     5581 atcggaggtg atatgcagcg ggccaatagg gatcacccgc gcatcgctat tgccctcatt
     5641 gataaacgga tacgtttcgg tatcagtcgt cggttcaggg cgcagtcgat aatccatcgc
     5701 atctttgcgc agcggatgca tatcttccgg ccagttatcg ggcaacacca ggcgacgctg
     5761 atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct cgccccagac
     5821 cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat cgaccagtgc
     5881 cttcaccacg atccagcatt tttcggcccc ttccattgaa agggcataat aaaccgcgta
     5941 atgaccgtta agtgtccgct cgtcgttgcc aaacaggact ggaagccagc catcatgttg
     6001 ataataaaga taatgtacaa cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg
     6061 ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt tcagggcggc
     6121 gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc tccgaaacag
     6181 atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg
     6241 tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt tgtggaatat
     6301 gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg gtcaaccagc
     6361 cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc gctgccatcc
     6421 gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata atcagcaggt
     6481 gattacgtgc cagtccggcg gtaatggtca taaattcgct aagaaaaatg ttgaagggcg
     6541 gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat ggcatgattt
     6601 tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt acattgccgg
     6661 aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa gatgtgcagc agcgcggcaa
     6721 aaattcccag cggcccgcca atgcctagcg ccaccgcgac cagccccatg ttctccacgc
     6781 tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat gccgccacgg
     6841 caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg ccgatggctt
     6901 ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc aatacggcgg
     6961 agagcagggc gctgaccgga ctcggcgctt cactgtgagc atccggcagc caggcgtgca
     7021 tgggaaatag cccggttttg gtaccaaagc caattagcac aaacacaaag gccaacagca
     7081 ttaatgttgg gtcaagcaag gacgattgct taagaacctc gctccagaat atcgccattt
     7141 ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg aacagaccaa
     7201 aagcgacacc aacagtacaa ataatgatgt acttccatgc tgattccagc gatgaacgct
     7261 gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc gccgcccaca
     7321 tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac agatggaaga
     7381 aaccgtagta atcgcacagc gtaacgggtg aaagctcgcc gtgtgccact tcatgacgca
     7441 tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg gcgaggaaca
     7501 aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct cctgcatcag
     7561 cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct aagctatgta
     7621 atacagtcac cgtacaagtc gcagagagcc cccgtttccg gcaggcaaaa cagagcagcg
     7681 aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc atctcattat
     7741 cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca tatttttctt
     7801 gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc tatttccacc
     7861 agttccggtg ctcgccaggc aagaagcgcc agcaccagat gggagccgtt ttccatcagg
     7921 cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag caggccaagc
     7981 agaaaatgac ctaacgctac cgccagcgcc ggtttcagcc cggtagccat cggtagcttc
     8041 acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag tgccatcatt
     8101 gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc agcgtaagtc
     8161 atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca gatcagcagt
     8221 tgctctgcgg cgaacaggca cgagagagtg gcgaaaatag acaccagcac cagcgactgg
     8281 caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa cagcgatgtc
     8341 agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca tcatctcctt
     8401 actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc agtacaatca
     8461 acaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg ctcggtttgc
     8521 ccggcacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc gattcaatca
     8581 tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca aaaccgacgg
     8641 caaacagcgg gaatttactg aagaagccgt tgaacggcgg cacaccggta atcgccagcg
     8701 ctgccacgca gaaacccacg cttggcaacg gcagggtgtg caatacgcca cgcagacgcg
     8761 gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa aacaggcttt
     8821 tagcgaacgc gtggttgacg atgtaggcga tgctaccctc cagcgccagc cgcgagccga
     8881 agatggagag cgacaagccg aagaacatcc agccaagttg agtgatggtc gaccaggcca
     8941 gcaaccgctt catatcctgc tgtggcaaat acatcagaaa gccataaaga atggtgacca
     9001 gcgccatgac catgccaacg ccgccaatca catgcgggat attgccgccg tcgataatgg
     9061 cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga taggcactga
     9121 tcggtgttgg cgcttccatt gcatccggta gccacgcttg catcggtagc tgggccgatt
     9181 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccgtgtaact
     9241 cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
     9301 acaaccccaa cgagccgata tgagtgataa gtaacgcttt tagtgctgaa agctgtgctt
     9361 tatcgctctg gtaataactg atcaacgccc aggagcagcc gcccgtaatt tcaaaaaaca
     9421 acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg ataaacacca
     9481 gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt ttatccgtca
     9541 gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca aacagaatca
     9601 gcgtactcat gcgatcaatg accaagccaa acaatgccac atcccccacg ttgaccaacg
     9661 tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagcgac aacatgcaca
     9721 gcgtggtcag tgcggcgaac aaaaccccca ttcggcggcc cgacgttgtg gcgaaaacga
     9781 aacgaccagt gcgccgataa aaggcagcat taacgtcgtc agagcaagat tttccatatt
     9841 ccgtcagtgc tccttacaga ccggttaacc agaagaccca tgcaagcgca gcaaggctga
     9901 agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg
     9961 cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca
    10021 gcaggcaggc gagagaaagt tcttgcgcgc gcccaaaggg cagaaacagg gccacaaaca
    10081 gtgctgccat cacgacctgt ttcagcccca gcccccattt cgctagcgcc agcccggcac
    10141 cggaatattc ggtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg
    10201 gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg
    10261 ttagcggcga gttccagccc gttgccagcg tattgctgat catctcgata tgcgtggaac
    10321 ctgctatcag cgccaatacc agcagtgaga gaatgagcat gggttcgacc agaatgccga
    10381 gcgtcaactc gcgactggca ccgactcccg caaacgggct tccggtatcc agccctgaaa
    10441 gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga tcgccgccgc
    10501 ccgcaaaagg ggaaacggta ataaatagtg gtagggccat cgccagcacc agcatgctgc
    10561 tgattaatac ccacggcatc aggcggaaca tcagacctga agatgtcggc gcatcttcct
    10621 ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg
    10681 agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagtggggtt agcatcagca
    10741 gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca
    10801 tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac
    10861 aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttggc cagccgttgg
    10921 gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc
    10981 ctggcggtga cgtgcgcaag acctttattt agcctcagcg tagggtcgag ctgttgtcgt
    11041 agacgaaata gcgcagaaaa gaccacacgc agcggctgca tcacgccatt gcctgacggg
    11101 gccatcgcat tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct
    11161 gcgcggcgcg aacgacacca cagccagtaa aggccaggca gcaaaggcat cgccagtagt
    11221 aacagcaaca gtaatgaagg agtgacccgt gtatgaaacg tgccgggtac aagtgctatt
    11281 ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatgtg cattatcttc
    11341 ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg
    11401 gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtgct gcgcggcgca
    11461 ccacagaaag taataccgta cattttgacg aagcacatta ctgccagccc accagtgact
    11521 gccagcatta ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt
    11581 agtgagaata gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaatg
    11641 gctgatatcg ccaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc
    11701 agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc acccgcgccg
    11761 agaaatagca gccctttgaa aagcgcatgg ttgagcagat gaaacagtgc gccgagcagt
    11821 ccaaccacgg tgagcagcgg gtcgtgcagt gacagaccga ccatcgccac accgaccgcc
    11881 agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt
    11941 tccgccagtg catacagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga
    12001 atgccccacc acagcggcaa acccgtttgc gccagcagat ccatcgccac tttcaggatg
    12061 ccgaaaatac cgattttgac cattacgcca gacatcaacg ctgaagcgtg cgatggtgcg
    12121 gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa
    12181 ccgaaaaagg ccagcaggaa caccgccgac gccagccccg gagaaagtga aagcgtgcgg
    12241 aaactggcaa aatcgaggct gccgctttcg cgccccatca gcaagaaggc gatcattatc
    12301 agcaccgatc cggcgtgggc gataaaaaag tagagcatgc cagcgttgat cgacgtttta
    12361 tcctgcctgg caatgaccag aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg
    12421 aaccaaaaag cgttgtccat caccagcagg gcaaccatcg atgcgatgaa aatattcata
    12481 aagaagccca tcgccgccgc gcctttgccc tcgtattcgc gcatataagt caatgaatag
    12541 agagaacaaa ccaccaccag caaggagata accagcacca taaatgccga gaggctatcc
    12601 attcgcaggg ttaaatcggc aaattcaaaa ggcgtggcaa aacgggcgac taaagttacg
    12661 cctgcatgta attgcgtaat tccgctgatg atgccaatca cgccgcccac cagcgatgtg
    12721 atgccggaaa gcttaatagc cagtctgtcc agaccgagta aaaacagcga agccagactg
    12781 gcaaacagat agaggatcag cgaccaggtt aataattgca gggcatccat tagcgttgct
    12841 cctgagtgag ggaaagggga tcctccccgc cattggcaaa ccagcttgcg gcaaggcgct
    12901 gtttttcttt catctggcgt tgcaggctat cgtcggtgat cagccgtaac gcctgattcg
    12961 ggcaagcgcg aacgcaggcc ggaccttctg gcaagaaatc acacaggtcg catttcaccg
    13021 cgacggtctg gacacccggt tcccagcgca gcaacgcatg ttggggcggc acgttttctt
    13081 cgccgtcttt gagtgagccc tcagcctgaa aaacatattg cgcaggggca ttcaccggac
    13141 ggcttcctga agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa
    13201 tacagaggct ttcgttgagt tggatcgcat catccctctg agagatggca ttgaccgggc
    13261 agacctgcag gcaaggggct tcctcacagt gatgacacac gacaggggca gtgagtgttg
    13321 acgtcttcgc cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg
    13381 aacaggcagc gagacaggta ttacatcctg tacaccacag tggttcggcc accacaaagc
    13441 ggttcatgtc ggctccttga aaatgatatg aaacattgca tttcatattg cataaaggat
    13501 gctattctga ttaactcaat atattcaata agttatgtat taactggatt ctgaacggta
    13561 caaaaatgat ggtcattaac gttcagcaaa aaatggattc gacacccgtg tcgcagggga
    13621 aagcgagaat gtgatcttga tcacgagaag tgaggtatgc tggccgcaag cgcagccagc
    13681 acggaatgga gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt
    13741 gattgctggt tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga
    13801 agctgatgcg atgaatgcca tcgtaggttt tgcccatgaa ggacttttca ccccagacgc
    13861 cgaattgttc gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt
    13921 tttccgcaaa acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc
    13981 ccgctttttt caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg
    14041 gggtcatggc tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat
    14101 taacttgttc tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca
    14161 gtggattcat tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat
    14221 actgccttgt gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc
    14281 gtccgcagat gcggggctgt gggcggttat ctgaatatgc aactgcgcag cccgttcatt
    14341 ttcagcaggt tgcgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag
    14401 tgctgtgaag cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga
    14461 tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag
    14521 ctcggcacct ttcaacggta acgttgattc aatcagagta atggcattcc atgaaccgga
    14581 aagcagcatg ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt
    14641 acagccgcaa ctactgacat gacgggtgat ggtgttcaca attccagggc gatcggcacc
    14701 caatgcagtg atcaccagat aatgttgcga tgacagtgtc aaactggtta ttcctttaag
    14761 gggtgagttg ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc
    14821 tgtctgcttg cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg
    14881 atggcccatg ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa
    14941 tgtctgtcgg gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc
    15001 gatcgtttct gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga
    15061 tgtggtgatg atgacgctgg agctggctga tgggcgcatt ccggtaattg ccgggaccgg
    15121 cgctaacgct actgcggaag ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt
    15181 cggctgcctg acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca
    15241 tttcaaagcc atcgctgagc atactgacct gccgcaaatt ctgtataatg tgccgtcccg
    15301 tactggctgc gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat
    15361 cggaatcaaa gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc
    15421 agatgatttt gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattagg
    15481 cggtcatggg gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg
    15541 caaactggca gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc
    15601 attacacaac aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga
    15661 actgggtctt gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg
    15721 tcgtgagacg gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt
    15781 tgatggctta ctctgttcaa aagtcgcgcc tggcaaaggt tgcgggtgtt tcgcttgttt
    15841 tattactcgc tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag
    15901 cctacctgga agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg
    15961 tgacctccgg tgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc
    16021 tggacattcg tccaccagcc cagccgctgg cactggtttc tggcgcgcgt acccagttca
    16081 cgggcgatac cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg
    16141 tggttagcgt gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga
    16201 cactgaccac cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc
    16261 gttatcaaat ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgttga
    16321 acctggaaca ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt tacagcacgg
    16381 agatgatgaa cgttatttcc gccggtctgg ataaatctgc cactgacgcc gcgaacgctg
    16441 cgcaaaatcg tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac
    16501 caatgctggt cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gcggcgctgg
    16561 aaaaagtggg catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt
    16621 ataagccgct gtctgacagc gactggcagg aactgggcgc gagcgatcca ggcctggcat
    16681 ccggtgacta taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg
    16741 atccgaaagg tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg
    16801 ctgcgtttag caagtaaaaa tacagggctg gaatcatccg gccctttttt ctgatatgat
    16861 acgcaaacgt gtgcgtcggc aggaaaacgc gattttagcg gtaattcgca cgaaatttgt
    16921 ttgtcggacg tagttcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc
    16981 agacagcaaa agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca
    17041 agctgagttg tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt
    17101 ggtgctcgaa ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga
    17161 tcgcaaaggt atgttgaaca acaagttcaa ctacttcatt atgagcaaac tggctgaagc
    17221 gggtatcccg actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct
    17281 ggatatggtg ccggttgagt gtgtcgtgcg taaccgtgct gcgggctctc tggtgaaacg
    17341 tcttggaatc gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa
    17401 cgatgccatg cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtgag
    17461 caaagagaac ctggcgcgta tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa
    17521 actgttcgat gatgctggtc tgattctggt cgacttcaag ctggagtttg gtctgtacaa
    17581 aggtgaagtg gtactgggcg atgagttctc cccggacggc agccgcctgt gggacaaaga
    17641 aacactggag aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaagc
    17701 ctatgaagcc gtcgcccgcc gcctgggtgt acagctggac tgatttttct gttcatcatc
    17761 ttgccgtgca gctggcacgg caagacaacc gctctcggaa tatatgcatt gaacgctcat
    17821 tcctccgcca tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac ctacgatcat
    17881 ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac
    17941 aatgtcgaag acaggcgtaa cagctccggc ggtccgtcaa tgggcggtcc cggttttcgc
    18001 ctgccaagcg gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc aggctactat
    18061 ggtgttgatt taaccgggtt gatgaccgga cagccggttt cccaacagca atcaacacgg
    18121 tcaattagcc caaatgaaga cgaagccgca aaattcacct cggtgattct ggcaaccacg
    18181 gaagacacct ggggacagca attcgagaag atgggtaaaa cctatcagca accgaagctg
    18241 gtcatgtatc gtggaatgac gcgtaccggc tgcggggcgg gccagtccat tatgggaccg
    18301 ttctattgcc cggcggatgg cacggtttat atcgatctct ccttctatga tgacatgaaa
    18361 gacaaactcg gcgcggatgg cgattttgcc caggggtacg ttatcgccca tgaagtcggt
    18421 catcatgtgc agaaattgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg
    18481 acgcaggcgg aagtaaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc
    18541 ggtgtctggg ggcataacat gcagcagcaa ggcgatctgg aagaggcgct gaacgcggcg
    18601 caggccatcg gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagacagt
    18661 ttcactcatg gtacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc
    18721 gatccggcac aatgcaatac ttttggtaaa agcatttaac ttccggggca aggatggctg
    18781 aactgactgc gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc
    18841 tggtgttgag cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct
    18901 tacctggcga ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttctccct
    18961 cggcgctaca aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg
    19021 gctttgatgc cgctgccttt gcggcactta gcggaacgtt gaaagcggga agctggctgg
    19081 ttttgttact ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct
    19141 ggagtgattg ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac
    19201 ttacggcgga taacgacgct attctctggc ggcaaaacca gccgttctcg ttggcgcatt
    19261 ttaccccccg tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac
    19321 tcttacagca gctactgacc atgccgccgg gcgtggcagc ggtaacggct gcgcgtgggc
    19381 gcggtaagtc cgcgctggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca
    19441 ccgcgcccgc aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct
    19501 ttattgcgcc ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgatg
    19561 aagccgcagc catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt
    19621 tgttaaccac tacggtgcag ggctacgaag gtaccggacg tggttttttg ctgaaatttt
    19681 gcgctcgctt tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg
    19741 gatgcccgct ggaaaaaatg gttagtgagg cactggtttt taacgatgaa aacttcaccc
    19801 atacaccaca aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgatc
    19861 cagaaacgcc gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acctcgccgc
    19921 tggatttacg ccggatgatg gatgcaccag ggcaacattt tttacaggcg gctggcgaaa
    19981 acgagattgc cggggcgttg tggctggtgg atgagggcgg attatctcaa gaactcagtc
    20041 aggcggtatg ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg
    20101 cgcacggcag caatccactg gcggcgacat tgcgcggacg gcgggtcagc cggattgcag
    20161 tccatccggc gcgtcagcgc gaaggcgttg ggcaacagct cattgccagc gctttgcaat
    20221 ataggcctgg cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt
    20281 tctggcaacg ctgcggtttt gtgctggtgc gaatgggtaa tcatcgtgaa gccagcagcg
    20341 gttgctatac ggcgatggca ctgttaccga tgagtgatgc gggtaaacag ctggctgaac
    20401 gtgagcatta ccgtttacgt cgcgatgcgc aagctctcgc gcagtggaat ggcgaaacgc
    20461 ttcctgttga tccactaaac aatgccgtcc tttctgacga cgactggctt gaactggccg
    20521 gttttgcttt cgctcatcgt ccgctattaa cgtcgttagg ttgcttattg cgtctgctac
    20581 aaaccagtga actggcatta ccggcgctgc gtgggcgttt acagaaaaac accagtgacg
    20641 cgcagttatg taccacactt aaactttcag gccgcaagat gttactggtt cgccagcggg
    20701 aagaggccgc gcaggcgctg ttcgcactta atgaggttcg cactgagcgt ctgcgcgatc
    20761 gcataacgca atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga
    20821 gtcagcggcg taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt
    20881 gttgttcaaa gcccggataa acctgcgcaa cagttgttac tgcttttcca cggtgtcggg
    20941 gataacccgg tggcaatggg ggaaataggc agctggtttg cgccgctatt tcctgatgcg
    21001 ctggtggtga gtgtcggagg cgcggagccg agcggtaatc cggcggggcg tcagtggttt
    21061 tcggtgcagg gtattacgga agataatcgc caggcgcgtg tggatgccat catgccgacg
    21121 tttattgaga tggtgcgcta ctggcagaaa cagagcggag taggggctaa cgccacagcg
    21181 ctaatcggtt tctctcaagg cgcgattatg gcgctggaga gcattaaagc cgaaccgggc
    21241 cttgcttcac gcgtcattgc ttttaacgga cgctatgcga gcctgccgga aactgcgtct
    21301 acagccacga caattcacct gattcatggc ggtgaagatc cggttatcga tctggcgcat
    21361 gcggttgcgg cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg
    21421 gaggatttag gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt
    21481 tataccattc cgaaacatta tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat
    21541 gatgtgattg aaatgatgta aaagacagat tacctggcct gataagacgc gtgagcgtcg
    21601 catcaggcat cgttcaccat tgccggatgc ggcgttaacg ccttatccgg cctacggttg
    21661 agcactcagc ttacttcttc ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact
    21721 tatcgttgaa atcacgatgc gggggaagct ccggcttgtt ggcgagaaat ttcttgtggt
    21781 cgacgcgctt gagatcttta atcacgttca gcaggacgcc taccaaaaag acaatcacca
    21841 gaatccacca atatttagcc agccagtcca tgcttatttc ctcttgcaga accactcatc
    21901 aggcgacgag ctgttccatg atacgttgat acatacgggc aagtagctgc aggtcggcag
    21961 cgttcacaca ttcattaatt ttatgaatag tggcattgac cggcccgagt tccaccacct
    22021 gcgcccccat gcgagcaata aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg
    22081 gtttaatttc attatagtgc tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg
    22141 cggtcaaaaa tggctgcccg gaaagccacc aatccaccgt atagcgcagt tgatgttttt
    22201 caagcagggc aagcacctgc gctttgatca tctcatcagt cagttcggtg ctgaagcgga
    22261 agttaaactg cacaaacagt tcacccggaa taacgttgtt actgcccgtt cccgcctgaa
    22321 tattggcaat ctgcatactg gtcgccggga agaattcatt gccctgatcc cactcaatag
    22381 ccactaattc attaaggaaa ggtgctgcgc gatgtactgg attgtcagcc agatgtgggt
    22441 aggccacatg cccctgcacg ccatgaatgg taaggttgca ggttaacgat ccgcgacgac
    22501 catttttcac cacatcacca accacttcga tactcgacgg ttcgccgacc agacagtaat
    22561 cgagacgctc attacgcgcc attaacgctt cgacgacttt taccgtaccg ttgtgggcac
    22621 tggcttcttc atcagaggtg atcagaaatg ccagtcgtcc cgtatggttg ggatgttgtg
    22681 cgacaaaacg ttctgctgct accaccatcg ccgccagcga gcctttcata tctgccgcac
    22741 cgcgcccgaa taacatgcca tcacgaatgg tgggttcaaa cggcggattg atccaacgat
    22801 cggcgtcgcc aggcggcacc acgtcggtat gcccggcaaa ggccaatgtt tcaccttgcc
    22861 cacgccatgc ccaaaaattc tgcgtatcgg caaagtccat gcgttcaacg gtaaaaccga
    22921 tcgcctgcaa acgatcaatc aacaaagcct ggcatcctgc gtcatcagga ctcagggaag
    22981 ggcggcgaat aagctgttgt gtcagctcaa taaccgggca cgacatagac tacacctcat
    23041 ggaaaaattg ctgataactg gaatcactga aacccagcag cataggctta ccgggcgcgc
    23101 agagcaatgg acgtttgata attgcaggca tttcagtcat taatgccgcc gctgaggccg
    23161 catcggtgat tttattgcgg gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt
    23221 tgagtaacgc ctcccaacct aattcgttga taaaaccgtt caataattcg ctgtccagcc
    23281 catcgacgcg gtaatcatga aaacgatagt cgatgttatt ggcttccagc caacggcgag
    23341 cctttttaat ggtgtcacaa tttttgatac cgtaaagtgt aaccataatg aatccttttt
    23401 cgatttatga gaataaatac tcatttaagg gtgaaaacat tttgcctgca aatgattatc
    23461 aggaaatata acattatcga agtattcaaa ttaacaacta ttaaataata ttaaacattt
    23521 ttaacgtaaa cattaagaat attcatcttg ttatctgtct gtattttaac ctgaattaaa
    23581 tcagtattag atgccgtatg aatatctcga aatgttgctg tgtatcacat acaaatcatc
    23641 accgcaggac catagtattt acataagatt tgtccccccg gaggtagtca tcgcagcaat
    23701 cgagtaaatt agcttcacca cggtatattg tttccgtaga atacccataa ttataagaga
    23761 ggttgttatg attgaacgtg aactggggaa ctggaaagac tttatcgaag ttatgcttcg
    23821 taagtaattc ctgagacaaa gtataaacag caagaacccg ctgagcaggt tcttaatcgg
    23881 aaagttgaaa aaggcgacac attggcatgt cgcctttttt attgcttatt ccgggcgcgg
    23941 ctttagcggg aagcggcggc gcaccagcac aaagaacagc ggcacgaagt aaatagccag
    24001 aatagtggct gaaatcatcc cgcccattac gccagtaccc accgcatgct gaccaccgga
    24061 accggcaccc gtgctggttg ccattggcaa tacgccgaag ataaatgcca gcgaggtcat
    24121 cagaatcggg cgtaaacgct gacggcaggc gtggagcgtc gcttcaaaca ggtcgtggcc
    24181 tttttggttc atctcgttag caaactcgac gatcaggatg gcgtttttcg ccgataaacc
    24241 aatgaccgtt aacaggccca cctggaagta aacgtcgttt tccagcccgc gcatccaggt
    24301 tgccagcagc gcgccgatta cccccagcgg cacgaccagc attaccgaga acggcaccga
    24361 ccagctttca tacaacgcag ccagacacag gaataccacc agcaaggaaa tggcgtacag
    24421 cgccggagcc tgcgcgccgg aaagtcgctc ctgatacgac atcgccgtcc actccagacc
    24481 aaagccgttt ggcagctgct tcactaacga ttccataata tccatcgccg taccggtact
    24541 gacccccggt gcggcttccc caacaatctc aaccgcagaa taaccgttat agcgttccag
    24601 acgcggcgag cctgtttccc agcgtgaggt cgcgaaagca gagaagggca ccatgccgcc
    24661 atctttattt cggacatacc agagattgat gtcatctggc agcatgcgat acggcgcagc
    24721 tgcctgcaca tagactttct tcacgcgacc gcgatccata aagtcattca catagctcga
    24781 accccaggcg gtttgcagtg tgtcgttaat atcgtcgata gcaacgccca gcgcctgagc
    24841 tttacgctgg tcgatatcaa tctgcaactg cggactgtcg tcgaggccgt tatggcgcac
    24901 acgggttagc tccgggtttt ccgccgccag cgccagcaac tggttacgtg ctgccatcag
    24961 cgcatcgtga cctgctccag cgtggtcctg caactccata tcaaaacccg cagaactacc
    25021 aagaccgcta attgctggcg ggctgctggc gataacgcga gcttctttaa tcttgttaaa
    25081 cgcctttgtt gcacgctcga taatggcaaa cgaggtgccg gtcttactgt cgcgttcgct
    25141 ccagtctttc aggcggataa acattcgcgc cacgttttgc ccgttacccc cagggccaga
    25201 accaacggtg gcaaacaccg acatgatgtt gtctttttca tgggtgaagt aatatttctc
    25261 gatttgctca acgactttca gggtctgttg ttgggttgaa ccgctgggca attgtaccga
    25321 ggtagtaaac atgccacggt cttccagcgg taagaacgac gtcggcaaac gcaggaacag
    25381 gaacaccatg ccgccaagca gcaggacata aatcacaatc cagcgcaggc tgcggtggag
    25441 aattttcgcc accccttttt cgtagcgttc ggcgttgcgg ttaaacatct ggttaaacca
    25501 ggcaaaaaag cctttttgcc cgtgatgctc accttttttc agtggcttaa gtagtgtggc
    25561 acacagagcc ggagtgagga tcatcgctac cagtactgac agcaccatcg ccgcaacaat
    25621 ggtaatagag aactggcgat agatggcacc ggtggtgccg ccgaagaagg ccattggtac
    25681 aaataccgcc gaaagcacca tcgcaatccc gaccagtgcc ccctggatct gccccatcga
    25741 tttacgtgtg gcttcgcgag gagtgagtcc ttcctcactc ataatacgtt cgacgttttc
    25801 caccaccacg attgcgtcat ccaccagcag accgatcgcc agtaccatcg cgaacatggt
    25861 taaggtgttg acgctgtaac cgaaggcgta aagtacggag aaggttccca tcaacaccac
    25921 cggcacggcg atagtgggta tcagcgtggc gcggaagttt tgcaggaaca gatacataac
    25981 gaggaaaacc agggcgatag cttccagcag cgttttcacc acgtcttcaa tggaggcttt
    26041 aacaaacgag gtggtttcat acgccacctt gtattccagt ccgtgcggga agtactgcgc
    26101 cagctcgtcg agacgattga gcaccagctc cgctgtcgcc atttcgtttg cgccggaggc
    26161 cagttttacc cccagcccgg aggctggctt accattgaag cggctaagat aatcgtattt
    26221 ctccgccccc atttcgacgg tggcgacatc gcccagcctt acctctgagc cgtcttgatt
    26281 cacccgcaag gtgatatcgc ggaactgttc tggtgtttgc agcagtgact gggcgttaat
    26341 ggtggcgttg agcgcctgct tatcgacgga aggtgtacca ccaagttgcc caaccgcaat
    26401 ctgcgcgttc tgtgactcaa tggcatcagt gacatcttta gccgtcatct ggaaactgtt
    26461 gagtttcgcc gggtccagcc agatacgcat ggaatattgc gaaccatagg catcgatatc
    26521 cccgacgcca ttcacgcggc ttaacgggtc ctgaatatta ctggcaacat aatcggcaat
    26581 atcctgttta tccatcgaac catcggtaga gacgaaagca atggtcagaa tattggtatc
    26641 gccggtttta cgcaccgtca cgccctgatt ttgcaccgcc tgcggtaact ttcgcatggc
    26701 tgattgcagc tggttttgta cttgctgcac ggcttcgtcc ggatcggtgc ctgctttaaa
    26761 acttaaagtg acagatgcct gaccgatgcc actgctctga gatgacatat acatgagatt
    26821 atcgaggccg gtcatatttt gctcgataac ctgggtcacg gtgttttcca gcgtctgggc
    26881 cgatgcgccg ggatagttag cggtcactcg cacattcggt ggcgcgagat cggggtattg
    26941 ttcaacgggc aatgaaaaaa tcgccagggt ccctgtcaga cacaacagga ttgccagcac
    27001 ccaggcaaaa atggggcgat caataaagaa attcgccatt aaaagaggac ctcgtgtttc
    27061 tgcgtatcgt tgtaggcttg acttgcttca ctgtagcggc aaggtacgag ccaatcgtgg
    27121 agaaaaaaag gagttaatgt aaattatcat gcccttttat actgaacctt aagtgcaagt
    27181 attctttggt cagtaggagg cattacatca actgactttc ctcaccctcc gcagagcgaa
    27241 agctaatact cactaacgtg ccgccgccgg aaggtggtga aaaagtcagc gtcccaccca
    27301 gccgttccgc gcgttcgcgc atgatattca gaccataatg accttcgggt tctttcggtt
    27361 caccgatacc aatcccgtta tcacggatat aaaccgtgtg attgccgtcc ggcgcggtga
    27421 cgcaactgac ggcgatttcg ctggcgttgg cgtgcttcat cgcattcagc accgcttcgc
    27481 gaataatttg caacaaatgc acctgcattt gcgcatccag cgccagggtt ggcagacggc
    27541 agtcgagggt cagtttggcg ctggtttgat tttgtaacgt atccagcatt tccctcaacg
    27601 cggaggggag atccgcctgc tgcagcgtca ggcggaaagt agtcaacagc tcgcgtaact
    27661 gccgataagc atcattcaac gcctgggaaa aatcggccat gatactttgt gcggtggcgt
    27721 tatcttccgg tatcgaacgc ttcagtaacg tcaactggat acgtaagtaa gaaagtacct
    27781 gagccagcga gtcgtgcaat tcgcgggcga tggttgcacg ttcttccatc aacaataact
    27841 gctgaaaatg cttttgcgcc tgattaaagt acaaaccgcg tcccagcatc gacgaaacgc
    27901 tgttaagcag cggttctgat gatgaaacgt gactattttg ccagtgcagt tcgccgtaaa
    27961 ccgtctcttg cattgtcacc ggtaaaatct gcatcggcaa ttccgggttt ggttgccctt
    28021 cgctaatccg ccagttttca ccgacattta actccagata ttcagccgct tcattgtcgc
    28081 gaacaatctg caaaatatgg cggaaacaat gcacatcaat ctggcttgtg ttcagcgcct
    28141 gcgaacattg atacaacacc tccagacgac gcttggcctc gtggagatcg cgggtctttt
    28201 cttctactga cgcctccagc gaaaggtaca atttatgcag ctcgctcgac atctggttaa
    28261 aggtttttgc aagcagacca agctcattcg gcagactggt atccagcggc ggcgagtcga
    28321 actgcccgtg ttcaatacgt tgactggcgg taaccagctg attcagcggg gcaaccacct
    28381 gatggcgtat acggcgcaaa gtaaaaaaga ccagcgtgaa aataccgata ccgccagcca
    28441 gggaaatcgc caccaccagc agcattttgc gttcagcgta gtgctgtaaa gccagcacga
    28501 acaggtctat ctgattaaca taattattaa tattggcctg ataccacggc aaatcgccct
    28561 tgctgagccg attattcatc tccagccagt tggcattcag atgcgcatag cgagttttta
    28621 ctgcttctgg cacataccag acgttgagat tggttaatac cgatgaatgc agtgcctgct
    28681 gaaacaactg gcgatgtgca ttgagttgtg gactgccact ttgcaagtcg tagcccaggc
    28741 ggtaactctg catacgcagc gatccggcaa tattgatagc ctcagcgtcg cgcaaactgc
    28801 tcgccagagt tagcagagcg atacccgtgg aaagaatcga cagcagcaca atgtaaaaaa
    28861 aggcccgggc cagactggcc gagacgggtc gtttaacaat cacacgcgtc ttctccacaa
    28921 aaaatcatta atgttccagt tcgctaaaac gcaatttagt cagtgatggg attatttgat
    28981 ctgccacagt ttatagcgaa atagttactt attctgggca catgaacatt gccgattccc
    29041 ctgtaagtcg ggtaataaca acactcatat aaagaataag gtttttacaa ccaaaaaaga
    29101 aggtcgttat gaatcgtttt attatggcca acagtcagca atgtctgggt tgtcatgctt
    29161 gtgaaatcgc ctgtgtcatg gctcacaatg atgagcaaca tgtcctgagc caacaccatt
    29221 ttcatccccg aattacggtt atcaaacatc agcagcaacg tagtgcagtg acctgtcacc
    29281 attgtgaaga tgcgccctgc gcccgtagct gccctaatgg cgcaatcagc cacgttgatg
    29341 acagcattca ggtcaatcag caaaagtgta ttggctgtaa atcctgcgtg gtggcctgtc
    29401 cgtttggtac gatgcaaatc gtcctgacac ccgtcgcggc aggaaaagta aaagccacgg
    29461 cgcataaatg cgacctttgt gcagggcgcg aaaacggtcc tgcctgtgtc gagaattgcc
    29521 cggcggacgc gctgcaactg gtcactgacg tcgcactctc cggcatggcg aaatcccgcc
    29581 gcttgcgcac cgcacgtcag gagcatcaac cgtggcatgc cagtaccgct gcacaagaaa
    29641 tgccggtaat gagtaaagtc gaacaaatgc aggcaacgcc cgcgcgtggc gagccggata
    29701 aactggcgat tgaagcgcgc aaaaccggtt ttgatgaaat ttatctgcca ttccgtgccg
    29761 atcaggcaca acgggaagcc tcgcgctgcc ttaaatgcgg cgagcacagc gtttgtgaat
    29821 ggacctgccc gctgcataac catattccgc agtggattga actggtgaaa gccggaaaca
    29881 tcgacgccgc cgtcgagctt tctcacctga ccaacaccct gccggaaatt accggacgcg
    29941 tttgtccgca ggaccgtttg tgtgaaggtg cctgtactat tcgcgatgag cacggcgcgg
    30001 taactatcgg caacattgaa cgctacattt cagatcaggc gttggcgaaa ggttggcgtc
    30061 ctgacttaag ccatgtcacc aaagtggaca agcgggtggc gattatcggt gcgggtccgg
    30121 cagggctggc ctgtgcggat gttctgaccc gcaatggcgt ggcggtgacg gtgtacgatc
    30181 gccatccaga aattggtggc ttgctcactt tcggcattcc ttctttcaaa ctggataaat
    30241 ccctgctggc acgccgtcgg gaaatcttca gcgcgatggg gattcacttc gaactcaatt
    30301 gtgaagtggg taaagatgtc tctttggatt cgcttttgga acaatacgac gcggtcttcg
    30361 ttggcgtagg cacttaccgt tccatgaaag cgggtttacc caatgaagat gcgccgggcg
    30421 tttatgacgc gctgccgttc ctcatcgcca acactaaaca ggtgatggga ctcgaagagc
    30481 taccggaaga gccgtttatc aataccgccg gactcaacgt cgtggtactg ggcggcggcg
    30541 acaccgcgat ggactgtgtg cgtaccgcgc tgcgccacgg cgcgagtaac gtcacctgcg
    30601 cttatcgtcg tgatgaagct aacatgccag gctcgaagaa agaggtgaag aacgcccgcg
    30661 aagagggggc caacttcgaa tttaacgtcc agccggtggc gcttgagctg aatgagcaag
    30721 gtcacgtttg cggaattcgt ttcctgcgca cgcgtcttgg cgagccggat gcccaggggc
    30781 gccggcgact tgtaccgatt gagggcagtg aatttgtcat gccagccgat gcggtgatta
    30841 tggtgtttgg cttcaatcca cacgggatgc cgtggctgga gtcgcacggt gtaacggtag
    30901 acaaatgggg ccgcatcatc gcggatgtgg aaagccagta ccgttaccag accaccaatc
    30961 cgaaaatctt cgccggtggc gacgccgtgc gtggtgcgga tctggtggtt accgcaatgg
    31021 cagaaggacg tcatgcggca caggggatta ttgactggct gggggtaaaa tcagtcaaat
    31081 ctcactgata gcctgcgcag acaaacccga cttcacagcg taatataatt gttcatttcg
    31141 cgctgtggag tcggtatgac gcaacaaatc accctcatta aagacaaaat tctctccgat
    31201 aactatttca ccctgcacaa cattacttac gatctcacgc gcaaagatgg cgaagttatc
    31261 cgccataaac gtgaagttta cgatcgcggt aatggtgcga cgatcctcct gtacaacgcg
    31321 aagaaaaaga ccgtggttct gattcgtcag ttccgtgtcg ctacctgggt taatggcaat
    31381 gaaagcgggc agctgattga aacctgcgcc gggctgctgg ataacgacga accggaagtg
    31441 tgcattcgta aagaagcgat tgaagagacg ggctatgaag tgggcgaagt gcgcaaatta
    31501 tttgaactgt atatgtcgcc tggcggtgtg actgagctaa tccacttttt tatcgccgaa
    31561 tacagtgaca atcagcgcgc taacgccggt ggcggtgttg aagatgaaga tattgaagtg
    31621 cttgagctgc cgttcagcca ggcgctggag atgatcaaaa ccggcgagat acgtgacggt
    31681 aagacggtgt tattgcttaa ctatttgcaa acgtcacatt taatggactg aaaaataaca
    31741 ataatatttc gttgtttatt attggctcaa tccgacaaat ctgattgagc cgcgctacct
    31801 gcgcaacgaa gatacgactt gtgctgtttg tttgaacttc tggggtcgta ccgtccatgc
    31861 gctatcgcat tttccttctc ttttttttcg ctttgttgcc gacgtctttg gtgtgggcgg
    31921 cactagcgca acgggcgttt tccgactggc aggtcacctg taataaccaa aacttctgcg
    31981 tggcgcgcaa tacgggcgat cataatggac tggtgatgac cctgagccgc agcgccgggg
    32041 cgcataccga tgccgtttta cgtattgagc gcggcgggtt gaagtcgccg gatgcgtctg
    32101 aaggagagat agcgccacgg ctgctgttag atggtgagcc gttggcgtta agtggtgaca
    32161 agtggcggat ttcaccatgg ttattagtaa ccgatgatac ggcaaccatc accgcgtttt
    32221 tgcagatgat tcaggaaggg aaggcgatca ccttacgcga cggcaatcag accatttctc
    32281 tgagtggctt aaaagcggcg ttgttgttta ttgatgctca gcaaaagcgc gttggcagtg
    32341 aaaccgcgtg gatcaagaaa ggggacgaac cgccgctcag cgtaccgccc gcgcctgcgc
    32401 tgaaagaggt cgcggtggtt aacccaacgc cgacgccact ctcactcgaa gaacgcaacg
    32461 atttgctgga ttatggaaac tggcggatga atggtctgcg ctgctcgctt gatccgttac
    32521 gtcgtgaggt gaatgtcact gcgctgactg atgataaagc gctgatgatg atcagctgtg
    32581 aggcaggggc ctataacacc attgatttgg catggattgt gtcgcgtaaa aagccgctag
    32641 cctcgcgccc ggttcggttg cgtttgccgt tcaacaacgg gcaggagacg aatgaactgg
    32701 aactgatgaa cgcaacattt gatgagaaat cgcgtgaact ggtgacctta gcgaaagggc
    32761 gcggattaag cgattgtggc attcaggcgc gctggcgctt tgacggtcag cgttttcgcc
    32821 tggtgcgtta cgccgcagaa cccacctgcg ataactggca tgggccagac gcctggccca
    32881 cgttgtggat cacccgttaa acaaaatggc ggggacatgt aggccggata agacgctgta
    32941 ggccggataa gacgcgtcag cgtcgcatcc ggcaatcagc atccggcgat cactatcagg
    33001 cacctttcac tcccagcacc ttatgcgctt ttgccacaat attctcggcg gtaaagccaa
    33061 agaacgggaa cagcttatcc gccggagcag attccccata acccgtcatc ccgacaattg
    33121 cccctttcag accaacatac ttgtaccagt aatcggcaat acctgcttcc accgccacgc
    33181 gagccgcaac gttagaaggc aacaccgact cccgatattc ctcatcctgg gcgtcgaaaa
    33241 tatcggtcga gggcagggaa accacgcgaa cattgcgacc ttctcctgct aatttctccg
    33301 cagcttgcag ggtgatttcc atctctgaac cggtggcaat cagaataata tctggcttac
    33361 cgccgctgtc tttcagcaca taaccgccac gagtaatctc tttaacctga tccggcgtac
    33421 gttccacctg ggccagattc tgccttgaga ggatcagtgc cgtcggtccg ttgtggcgct
    33481 caaccgccag cttccagccc accgccgctt ccacctgatc gcacggtcgc caggtgctga
    33541 aatttggcgt taagcgcagg ctggccagtt gctcaacagc ctggtgcgtc ggaccatctt
    33601 cgcccaggcc aattgagtcg tgggtataaa ccataatctg ccgcgctttc atcagtgccg
    33661 ccatccgcgc ggcgttacgg gcgtattcaa caaacatcag gaacgtcgcg gtatacggca
    33721 caaagccgcc gtggtgcgcg atgccgttgg cgatagcggt catgccaaat tcacgcaccc
    33781 cgtagtgaat gtagttgccc gccggatctt ccttcagcga aacagaacct ttccagatgg
    33841 tcaggttgct gggagccaga tccgccgaac cgccgagcag ctccggtagc aaggggccat
    33901 aagcgttaag cgtattttgc gaagccttac gggtggcgat tttcgccgga ttcgcctgca
    33961 actcattgat atacttctga gtcgttttct cccagtcctt cggtaaacca ccgctcatcc
    34021 gacgggtaaa ctcttctgcc agttgcggat gagccttttt ataggcggca aacttctcat
    34081 tccacctctg ctgcgctttt tcgccttttt cgcgggcatc ccaggcgtga taaatatctt
    34141 tagggatctc aaatggcgga tggtgccagc ccagtttttg ccgtgccagc gccacttctt
    34201 cttcccccag cggtgcgccg tgcgcctctt ccttacctgc tttattcggc gatccaaagc
    34261 caatcaccgt acgacagata atcagcgacg gcttatcttt cacgctttgc gcttcaagga
    34321 tcgcttcctt caccgcctgc ggatcgtgac cgtcgatttc atggatcaca tgccagtgat
    34381 aggcttcaaa acgttttgcc gtatcgtcgg taaaccagcc ttctgtttca ccgtcgatgg
    34441 aaataccgtt gtgatcgtaa aaaccaatca gcttgcccag tcccagcgtg cctgccagcg
    34501 aacagacttc gtgggaaata ccttccatca ggcagccgtc gcccataaac acataggtga
    34561 agtgatcgac gatctcatgg tctggctggt taaactgcgc cgccagtgta cgctctgcta
    34621 tcgccagccc gacggcgttc gccaaacctt gtccaagtgg accggtggtg gtttcaacgc
    34681 ctggcgtata accaatctcc gggtggcctg gggttttcga atgcagttga cgaaagttct
    34741 tcagctcttc cagcggcagg tcgtaaccgg tcagatgcag caaactgtag agcagcatcg
    34801 acgcgtgacc gttggaaaga ataaagcggt cgcgatcata ccaggttggg tcggtagggt
    34861 tatgtttaag aaaatcgttc cacagcactt cggcaatatc agccatgccc atcggcgcgc
    34921 cgggatgacc agaattggct ttttgtaccg catccatact gagtgcgcga atcgcattgg
    34981 caaggtcttt tcgggacata taacactccg tggctggttt atagtttggc ggcaagaaga
    35041 tcttccagtt tgcgttgatc aacggcgaac agacgaatgc cttcagacag tttttctacc
    35101 gccatcgcat actgattgtg ctcccaacgg aactccgctt cgctcatggg agctgggcgt
    35161 gggaacgtct gagaaggtgg gattaattta cgtaccactg gcgaaacttt ttcctgcagc
    35221 tccttcagca aattcggtgc gatagtcagt cgatcgcagc cggttaaggc gaggatttgt
    35281 tcggtgcgac ggaagctcgc gcccatcaca atggtttcat agtggtgttg cttatagtag
    35341 tcgtagatat tgcgcaccga tttaacgccc ggatcttctt ccaccacata cgggtccatc
    35401 ggcttgcgtg cctgatacca gtcataaata cgcccgacaa acggcgaaat cagaaaaacg
    35461 cctgcttccg cacaggcccg tgcctgtgca aaagaaaaca gcagcgtcag gttgcagtta
    35521 ataccttctt tttccagctc ttctgccgcg cgaattcctt cccaggtcga agccagcttg
    35581 atcagaatgc gtgatttctc aacgccttgt tgctgataca agtccaccag atggcgcgcc
    35641 ttctcaatac tcttttcttt atcaaaagag aggcgtgcat caacttctgt tgacacgcga
    35701 ccgggtacga ttttgaggat ttcagcaccg aaattgaccg ccagtttgtc acacgctgcg
    35761 accacctgtt gttcctgggt cttgccattt tttttacccc aggcgatagc atcgtctatt
    35821 aaatgctcat attgtgataa tccggcagcc ttgagtaaca gcgaaggatt ggtggtggca
    35881 tcctggggat gataatggcg aatggactca atatcgccgc tgtctgccac gacagtggtg
    35941 aactgtttga tgccgtctaa ctcgttcata ggaaatactc cttgaaaagt aaagtgttag
    36001 atgagtgcgt taattcacac ttctgagaaa tttcgctaaa cgcatcaaaa aagcatagca
    36061 gacaggcatg gtattgctgg attaagcagg taacatcagt gttataggat tattaccaaa
    36121 acattatatt aatggtgaga gtttggactt gctcaaagtc tgtagactcc ggcaaggtaa
    36181 taatgtgcgc cacgttgtgg gcagggggtg ttgcccacac actttatttg tgaacgttac
    36241 gtgaaaggaa caaccaaatg gatgaccagt taaaacaaag tgcacttgat ttccatgaat
    36301 ttccagttcc agggaaaatc caggtttctc caaccaagcc tctggcaacg cagcgcgatc
    36361 tggcgctggc ctactcaccc ggcgttgccg caccttgtct tgaaatcgaa aaagacccgt
    36421 taaaagccta caaatatacc gcccgcggta acctggtggc ggtgatctct aacggtacgg
    36481 cggtgctggg gttaggcaac attggcgcgc tggctggcaa accggtgatg gaaggcaagg
    36541 gcgttctgtt taagaaattc gccgggattg atgtatttga cattgaagtt gatgaactcg
    36601 acccggacaa atttattgaa gttgtcgctg cgctcgaacc gaccttcggc ggaatcaacc
    36661 tcgaagacat taaagcgcca gaatgtttct atattgaaca gaaactgcgc gagcggatga
    36721 atattccggt attccacgac gatcagcacg gcacggcaat catcagcact gccgccatcc
    36781 tcaacggctt gcgcgtggtg gagaaaaaca tctccgacgt gcggatggtg gtttccggcg
    36841 cgggtgccgc agcaatcgcc tgtatgaatc tgctggtagc gctgggtctg caaaaacata
    36901 acatcgtggt ttgcgattca aaaggcgtta tctatcaagg ccgtgagcca aacatggcgg
    36961 aaaccaaagc cgcatatgcg gtggtggatg acggcaaacg taccctcgat gatgtgattg
    37021 aaggtgcgga tattttcctg ggctgttccg gcccgaaagt gctgacccag gaaatggtga
    37081 agaaaatggc tcgggcacca atgattctgg cgctggcgaa tccggaaccg gaaattctgc
    37141 cgccgctggc gaaagaagtg cgtccggatg ccatcatctg caccggtcgt tctgactatc
    37201 caaaccaggt caacaacgtc ctttgcttcc cgttcatctt ccgtggcgcg ctggacgttg
    37261 gcgcaaccgc catcaacgaa gagatgaaac tggcggcggt acgcgcgatt gcagaactgg
    37321 cccatgcgga acagagcgaa gtggtggctt cagcgtatgg cgatcaggat ctgagctttg
    37381 gtccggaata catcattcca aaaccgtttg atccgcgctt gatcgttaag atcgctcctg
    37441 cggtcgcgaa agcggcgatg gagtcgggcg tggcgacgcg tccgattgct gatttcgacg
    37501 tctacatcga caagctgact gagttcgttt acaaaaccaa cctgtttatg aagccgattt
    37561 tctcccaggc tcgcaaagcg ccgaagcgcg tagttctgcc ggaaggggaa gaggcgcgcg
    37621 ttctgcatgc cactcaggaa ctggtaacgc tgggactggc gaaaccgatc cttatcggtc
    37681 gtccgaacgt gatcgaaatg cgcattcaga aactgggctt gcagatcaaa gcgggcgttg
    37741 attttgagat cgtcaataac gaatccgatc cgcgctttaa agagtactgg accgaatact
    37801 tccagatcat gaagcgtcgc ggcgtcactc aggaacaggc gcagcgggcg ctgatcagta
    37861 acccgacagt gatcggcgcg atcatggttc agcgtggcga agccgatgca atgatttgcg
    37921 gtacggtggg tgattatcat gaacatttta gcgtggtgaa aaatgtcttt ggttatcgcg
    37981 atggcgttca caccgcaggt gcaatgaacg cgctgctgct gccgagtggt aacaccttta
    38041 ttgccgatac ctacgtcaat gacgaaccag acgcagaaga gctggcggag atcaccttga
    38101 tggcggcaga aactgtccgt cgtttcggta ttgaaccgcg cgttgctctg ttgtcgcact
    38161 ccaactttgg ttcttctgac tgcccgtcgt cgagcaaaat gcgtcaggcg ctggaactgg
    38221 tcagggaacg tgcgccagaa ctgatgattg atggtgaaat gcacggcgat gcggcgctgg
    38281 tggaagcgat tcgcaacgac cgtatgccgg acagcccttt gaaaggttct gccaatattc
    38341 tggtgatgcc gaacatggaa gctgcccgca ttagttacaa cttactgcgc gtttccagct
    38401 cggaaggtgt gactgtcggt ccggtgctga tgggcgtggc gaaaccggtt cacgtgttaa
    38461 cgccgatcgc atcggtgcgt cgtatcgtca acatggtggc gctggccgtg gttgaagcgc
    38521 aaacccaacc gctgtaattc ttttttaact ctcacgctta ccctgaatat tcagggtaag
    38581 cggtttggct gcaatatatt agtaaagctt attactgagt ttgcgaataa taaaaaaagc
    38641 agtctatata atatctcgat attattttat ttatattcat gcgttgcata tgaaagttta
    38701 tgcaccacag cgaatatctc tccattctta gtgatctacc tcacctttta aacgcgcttg
    38761 ccgaattttg ttatttactc tgacgaaaaa ttgtcacgat acacgaaagt ttttcacagg
    38821 cggcgactca tggataaaga acgcatcatt caggaatttg tgcccggcaa acaggtcaca
    38881 ctggcgcatc tcatcgcgca tcccggtgag gaactggcga agaaaatcgg cgttccggat
    38941 gcgggcgtaa ttggcattat gacgctaact cccggcgaaa cggcgatgat cgtcggtgat
    39001 ttagcgctga aagccgctga tgtgcatatc ggttttctcg accgcttcag cggtgcattg
    39061 gtgatttatg gctcggtggg cgcagtagaa gaagcgttat cacaaaccgt cagcggttta
    39121 ggtcgcttat taaattatac gctttgtgaa atgaccaaaa gttaatacga ggcattcatg
    39181 aaacgaattg cgtttgtggg tagcgtcggg gcaggaaaaa caacgttatt taatgcgttg
    39241 caggggaatt ataccctcgc cagaaaaaca caggccgtgg aatttaatga taagggcgat
    39301 attgatacgc cgggtgaata ttttagccat ccccgctggt atcacgcctt aattaccacg
    39361 ctacaggatg tagacatgct gatctatgtc catggcgcaa atgacccgga aagtcggttg
    39421 cctgccgggt tgctggatat tggcgtcagc aaacggcaaa tcgccgtcat cagtaaaacg
    39481 gacatgccag atgctgacgt cgccgcaacg cgaaagttat tgcttgagac gggtttcgaa
    39541 gagccgattt tcgagctaaa cagccacgac ccgcaaagcg tgcagcaact ggtggattat
    39601 ctggcatctc tcaccaaaca ggaggaagca ggtgaaaaaa ctcatcacag cgaatgatat
    39661 tcgtgaagca cacgcgcgcg gcgaacaggc aatgtccgtg gttctgcgcg ccagcattat
    39721 taccccggaa gcccgtaaag tggcggatct actgggtttt accatcaccg aatgtgatga
    39781 atcgatcccg gtaacggcgt ccgtgcccgc cagcgtgcca gccgacaaaa ccgaaagcca
    39841 gcgcatccgc gaaaccatca ttgcccagct gccggaaggc cagtttaccg aaagcctggt
    39901 cgcacagctg atggaaaaag tgatgaagga aaaacagtcg ctggagcagg gcgcaatgca
    39961 gccgagcttc aaatcggtga ccggcaaagg cggcatcaaa gtgattgatg gcagcagcgt
    40021 caagtttggt cgttttgatg gcgcacaacc gcattgcgtg ggcttgactg atcttgtgac
    40081 cggagacgac ggcagcagca tggccgccgg ttttatgcag tgggagaacg cattcttccc
    40141 gtggaccctg aactacgacg aaatcgacat ggtgctggaa ggggagctcc atgttcgcca
    40201 cgaaggcgaa accatgatcg ccaaagcagg cgacgtgatg tttatcccga aaggctccag
    40261 catcgaattt ggtacgacat ccagcgtgaa attcctgtat gtcgcctggc cggctaactg
    40321 gcaatcccta tgaaagattt catcaccgaa gcatggctaa gagcgaacca tacgctcagc
    40381 gaaggcgcag agatccatct ccctgcggac agtcgcctga cgccctctgc ccgggagttg
    40441 ctggaaagcc gccatctgcg catcaagttt attgacgagc agggccgcct gtttgttgac
    40501 gatgaacagc agcagccgca gcccgttcat gggctgacca gtagcgatga acatccgcag
    40561 gcgtgctgcg aactgtgccg ccagccggtg gcaaaaaagc cggatacgct gactcacctg
    40621 tcggcggaaa aaatggtcgc caaaagcgat ccgcgtctgg gttttcgcgc ggttctcgac
    40681 agcaccattg cgctggcagt gtggctgcaa attgaactgg cggaaccgtg gcagccgtgg
    40741 ctggcggata tccgttcgcg tcttggcaac attatgcgcg ccgatgcgct gggggaaccg
    40801 ctgggctgcc aggcgattgt cgggctttct gacgaagatc tgcatcgact ttctcaccag
    40861 ccgctgcgct atctcgacca cgatcatctg gtgccggaag ccagccacgg gcgcgatgcg
    40921 gcgttgctca atctgctacg taccaaagtg cgggaaaccg aaaccgtagc ggcgcaggtg
    40981 tttatcaccc gcagctttga agtattacgc ccggacatct tgcaggcgct gaaccgcctt
    41041 tcgagcacgg tctacgtaat gatgatcctg agcgtaacga agccgccgct aacggtgaag
    41101 cagatccaac aacgactggg ggaaacgcaa tgattattga acgttgtcgt gaactggcgt
    41161 tgcgagcgcc cgccagagtg gtttttccgg atgcgttaga tcaacgtgtg ctgaaagctg
    41221 cgcaatattt acatcaacaa ggtctggcaa cgcccattct ggtcgctaat ccgtttgaac
    41281 ttcgtcagtt tgcgctcagt cacggcgtgg cgatggacgg gctacaggtg attgatccgc
    41341 atggcaacct cgcaatgcgg gaagaatttg ctcatcgctg gctggcccgc gcgggcgaaa
    41401 aaacgccgcc ggatgcgctg gaaaaactta ccgatccgct gatgttcgcc gcagcaatgg
    41461 tcagcgccgg taaagcggat gtctgtatcg cgggcaacct ctcttccacg gcgaatgtgc
    41521 tgcgtgccgg attacgtatt atcggcttgc agccaggctg taaaacgctc tcatccattt
    41581 tcctgatgct gccacagtac agcggtccgg cgttgggctt tgccgattgc agcgtggtgc
    41641 cacagccgac ggcggcgcaa ctggcggata tcgcgcttgc cagtgccgaa acctggcgcg
    41701 ccctcaccgg agaagagccg cgcgtggcga tgctgtcgtt ttccagtaac ggtagcgccc
    41761 gtcacccctg cgttgccaac gtccagcagg cgacagaaat cgtccgtgag cgcgcaccaa
    41821 agctggtggt tgatggcgag ttgcagtttg acgccgcctt cgtgccggaa gtggcggcgc
    41881 aaaaagcgcc tgccagcccg ctacagggca aagccaatgt gatggttttt ccgtcgctgg
    41941 aagccggaaa tattggctac aaaatcgcac aacgactcgg cggatatcgt gccgtcgggc
    42001 cattgataca aggacttgcc gcgccgatgc acgatctctc tcgtggttgt agcgtgcagg
    42061 aaattatcga gctggcgctg gtggcagctg tgccgcgtca gacagaagtg aaccgcgaaa
    42121 gcagtttaca aacactggtt gaatgaacgg tcccgttctg gaccctgata aagaggaaag
    42181 cacaatggaa gcattaggaa tgatcgaaac ccggggcctg gttgcactga ttgaggcttc
    42241 tgacgcgatg gttaaagctg cgcgcgtcaa gctggttggt gttaagcaga ttggcggcgg
    42301 cctgtgtact gccatggttc gtggtgatgt tgcagcctgt aaagccgcga ccgatgcagg
    42361 tgccgcagcg gcacagcgta tcggcgagct ggtatctgta cacgttatcc cgcgtccgca
    42421 cggcgacctg gaagaagtct tcccgatcgg cctgaaaggc gatagcagca acctgtaatc
    42481 accctgtgtg acgtcgattc gggcgtcacg ctctctcctt tttcatttta ccttctgcgg
    42541 agggtagggg gagcttttac ctgaaatacg ccacggaggc gggtatgaaa ctggcagtcg
    42601 tcactggaca aattgtttgt accgtacgcc atcacggact ggcgcatgac aaattgctga
    42661 tggtggaaat gattgatcca caaggtaatc ctgacgggca atgcgccgtc gccatcgaca
    42721 atattggcgc gggaaccggg gagtgggtgt tactggtgag tggcagttcc gcccgccagg
    42781 cgcataaaag cgaaacgtca ccggtcgatc tgtgcgtgat tggcattgtc gatgaggtgg
    42841 tgtctggcgg tcaggtaatt ttccacaaat aaggcagaac atcatgaatc aacaggatat
    42901 tgaacaggtg gtgaaagcgg tactgctgaa aatgcaaagc agtgacacgc cgcccgccgc
    42961 cgttcatgag atgggcgttt tcgcgtccct ggatgacgcc gttgcggcag ccaaagtcgc
    43021 ccagcaaggg ttaaaaagcg tggcaatgcg ccagttagcc attgctgcca ttcgtgaggc
    43081 aggcgaaaaa cacgccagag atttagcgga acttgccgtc agtgaaaccg gcatgggccg
    43141 cgttgaagat aaatttgcca aaaacgtcgc tcaggcgcgc ggcacgccag gcgttgagtg
    43201 tctctctccg caggtgctga ccggcgacaa cggcctgact ttaattgaaa acgcgccctg
    43261 gggcgtggtg gcttcggtaa ctccgtccac taacccggcg gcaaccgtaa ttaataacgc
    43321 catcagcctg attgccgcgg gcaacagcgt catttttgcc ccgcatccgg cggcgaaaaa
    43381 agtctcccag cgggcgatca cgctgctcaa ccaggcgatt gttgccgcag gcgggccgga
    43441 aaacttactg gttactgtgg caaatccgga tatcgaaacc gcgcagcgtt tgttcaagtt
    43501 tccgggcatc ggccttctgg tggtgaccgg cggcgaagcg gtagtagaag cggcgcgtaa
    43561 acacaccaat aaacgtctga ttgccgcagg cgctggcaac ccgccggtag tggtggatga
    43621 aaccgccgac ctcgcccgtg ccgctcagtc catcgtcaaa ggcgcttctt tcgataacaa
    43681 catcatttgt gccgacgaaa aggtactgat tgttgttgat agcgtagccg atgaactgat
    43741 gcgtctgatg gaaggccagc acgcggtgaa actgaccgca gaacaggcgc agcagctgca
    43801 accggtgttg ctgaaaaata tcgacgagcg cggaaaaggc accgtcagcc gtgactgggt
    43861 tggtcgcgat gcggcgaaaa tcgcggcggc aatcggcctg aacgtgccgc aagaaacgcg
    43921 tttgctgttt gtggaaacca ctgcagaaca tccgtttgcc gtgaccgaac tgatgatgcc
    43981 ggtgctgccc gtcgtgcgcg tcgccaacgt ggcggatgcc attgcgctgg cggtgaaact
    44041 ggaaggcggt tgccaccaca cggcggcaat gcactcgcgc aacatcgaaa acatgaacca
    44101 gatggcgaat gctattgata ccagcatttt cgttaagaac ggaccgcgca ttgccgggct
    44161 ggggctgggc ggggaaggct ggaccaccat gaccatcacc acgccaaccg gtgaaggggt
    44221 aactagcgtg cgtacgtttg tccgtctccg tcgctgtgta ttagtcgatg cgtttcgcat
    44281 tgtttaagga gtaacagatg gcgcacgacg aacaatggct caccccacgt ctgcaaacgg
    44341 cggcaacgct gtgtaaccag acgcccgccg cgacggaatc tccgctgtgg ctgggggtgg
    44401 atctgggcac ctgcgatgtg gtgtcgatgg ttgtcgaccg cgacggtcag tcggtggcgg
    44461 tatgcctcga ctgggccgat gtggtccgcg acggcatcgt ctgggatttc ttcggcgctg
    44521 tcaccattgt tcgtcgccat ctcgacacgc tcgagcagca attcggtcgc cgttttagcc
    44581 atgcggcgac ttcatttcct ccaggtacgg acccgcgtat ttccatcaac gtgctggagt
    44641 ctgctggact ggaagtgagc catgtgctgg atgagccaac cgcagttgcg gatctgctgc
    44701 aactggacaa cgccggtgta gtggatatcg gcggcggcac taccggcatt gccatcgtga
    44761 aaaagggcaa ggtgacgtac tcggcggatg aagcaaccgg cggacatcac atctctctga
    44821 ctctcgccgg aaatcgccgt atttcgctgg aagaggcgga gcagtacaag cgcggtcacg
    44881 gtgaagagat ttggcctacg gtgaaaccgg tgtacgaaaa aatggcggac attgtcgccc
    44941 ggcatattga agggcagggc attactgatt tatggctggc gggcggctct tgtatgcaac
    45001 cgggcgtggc ggaactgttt cgcaaacaat tcccggcgtt acaggtgcat ttaccgcagc
    45061 acagtttgtt tatgaccccg ctggcgatcg ccagtagtgg gcgagagaaa gcggaggggc
    45121 tatatgcaaa atgaattgca gaccgcgctc tttcaggcgt tcgataccct gaatctgcaa
    45181 cgggtaaaaa catttagcgt tccaccggtg acgctttgcg gtccgggctc ggtgagcagt
    45241 tgcggacagc aagcgcaaac gcgtgggctg aaacatctgt tcgtgatggc agacagcttt
    45301 ttgcatcagg cagggatgac cgccgggctg acgcgcagcc tggctgttaa aggcatcgcc
    45361 atgacgctct ggccatgtcc ggtgggcgaa ccgtgcatca ccgacgtgtg tgcagccgtg
    45421 gcgcagttgc gtgagtcagg ctgtgatggg gtgatcgcat ttggcggcgg ctcggtgctg
    45481 gatgcggcga aagccgtggc gttgctggtg acgaacccgg atagcacgct ggcagagatg
    45541 tcagaaacca gcgttctgca accgcgcttg ccgctgattg ccattccaac gaccgccgga
    45601 accggctctg aaaccaccaa tgtaacggtg attatcgacg cggtgagcgg gcgcaagcag
    45661 gtgttagccc atgcctcgct gatgccggat gtggcgattc tcgacgccgc gttgaccgaa
    45721 ggtgtgccgt cgcatgtcac ggcgatgacc ggcattgatg cgttaaccca tgccattgaa
    45781 gcatacagcg ccctgaacgc tacaccgttt accgacagtc tggcgattgg tgccattgcg
    45841 atgattggca aatcgctgcc gaaagcggtg ggctacggtc acgaccttgc cgcgcgcgag
    45901 agcatgttgc tggcttcatg tatggcggga atggcgtttt ccagtgcggg tcttgggttg
    45961 tgccacgcga tggcgcatca gccgggcgcg gcgctgcata ttccacacgg tctggcgaac
    46021 gccatgttgc tgccaacggt gatggaattt aaccggatgg tttgtcgtga acgctttagt
    46081 cagattggtc gggcactgcg aactaaaaaa tccgacgatc gtgacgctat taacgcggta
    46141 agtgagctga ttgcggaagt tgggattggt aaacgactgg gcgatgttgg tgcgacatct
    46201 gcgcattacg gcgcatgggc gcaggccgcg ctggaagata tttgtctgcg cagtaacccg
    46261 cgtaccgcca gcctggaaca gattgtcgga ctgtacgcgg cggcgcaata aatgccggat
    46321 gcgacgcttg ccgcgtctta tccagcctac gggattgcac atgtagggcg gataaggcgt
    46381 tcacgccgca tccgccagtg gcgcggtgca attgccggat gcgatgcttg ccgcgtctta
    46441 tccggcctac gggattgcac atgtagggcg gataaggcgt tcacgccgca tccgccagtg
    46501 gcgcggtgca attgccggat gcgacgcttg ccgcgtctga tccggccttc gggattgcac
    46561 atgtagggcg gataaggcgt ttacgccgca tccgccaata aataacaact aacagggagt
    46621 aaaggcgatg ggaattaacg aaatcatcat gtacatcatg atgttcttta tgctgatagc
    46681 tgccgtagac aggatcctgt cgcagttcgg cggttctgct cgtttcctcg gtaagttcgg
    46741 taaaagtatc gaaggatcag gcggtcagtt cgaagaaggc tttatggcaa tgggcgctct
    46801 gggtctggcg atggtcggta tgaccgcgct ggcaccggta ctggctcacg tactcgggcc
    46861 ggtaattatc ccggtttacg aaatgctcgg cgcaaacccg tcaatgttcg ccggaacgct
    46921 gctggcgtgc gatatgggcg gcttcttcct cgccaaagag ctggcgggcg gcgacgtagc
    46981 agcgtggcta tactctgggt taattcttgg gtcgatgatg gggccaacga ttgtgttttc
    47041 cattccggtg gcgctcggca ttatcgaacc ttctgaccgt cgttatctgg cgctcggcgt
    47101 gctggcgggc attgtgacca ttccgattgg ctgtattgct ggtggtctgg ttgctatgta
    47161 ctccggtgtg cagatcaacg gtcagccggt ggaattcact ttcgccctga tcctgatgaa
    47221 catgatcccg gtgatcattg ttgcgattct ggtggcgctg gggctgaaat tcatcccgga
    47281 aaaaatgatc aacggcttcc agatcttcgc caaattcctc gttgcattga tcaccctcgg
    47341 tcttgctgcc gcggtagtta agtttctgct tggctgggaa ctgatccccg gtctggaccc
    47401 tatctttatg gcccctggcg ataaacccgg tgaagtgatg cgcgccattg aagttatcgg
    47461 ttctatctcc tgcgttctgt taggggcgta tccaatggtg ctgctgctga cccgctggtt
    47521 tgaaaaaccg ctgatgagcg tcggtaaagt gctgaatatg aacaacatcg cggcagccgg
    47581 catggtggca acgcttgcca acaacatccc gatgttcggc atgatgaagc agatggatac
    47641 ccgcggcaaa gtcatcaact gcgccttcgc cgtttccgct gctttcgccc tgggcgacca
    47701 cttaggcttc gccgctgcca acatgaacgc catgatcttc ccgatgattg tcggcaagtt
    47761 gatcggcggc gtaacggcga ttggcgtggc gatgatgctg gtgccaaaag aagacgcgac
    47821 cacggctaaa accgaagcgg aggcacaatc gtgaacactc gccagctatt gagcgtcggt
    47881 atcgatatcg gcaccaccac cacccaggtg attttctccc acctggagct ggttaaccgt
    47941 gcggcggtgt cgcaggtgcc gcgctacgaa ttcattaaac gcgaaattag ctggcaaagc
    48001 ccggtgttct ttacccctgt cgataaacag ggtggtttaa aagaagcgga actgaaaacc
    48061 ttaatcctcg agcaatatca ggctgcgggc attgcgccgg aaagcgttga ttctggtgcc
    48121 atcatcatca ccggtgaaag cgcgaaaacc cgcaatgctc gtccggcggt gatggtgctc
    48181 tcccagtcgc tgggcgattt tgtcgttgcc agcgccgggc cgcacctcga atccgtcatt
    48241 gccggtcacg gtgccggggc acaaaccctt tctgaacaac ggctgtgtcg ggtactgaat
    48301 attgacatcg gcggtggcac tgcgaactac gccctgttcg atgccggaaa aatcagcggc
    48361 actgcctgcc tcaacgtcgg tggtcgcctg ctggaaaccg acagccaggg gcgcgtggtt
    48421 tacgctcata aaccggggca gatgattgtg gatgagtgct tcggcgcagg caccgacgcc
    48481 cgttcgctga ccggcgcgca gctggtgcag gttacccggc ggatggcaga actgattgtc
    48541 gaagtgattg acggaacgct ctcgccgctc gcgcaggcat tgatgcaaac cggtttgctg
    48601 cccgcaggtg ttacgcccga aatcattacg ctttctggcg gcgtgggcga atgttatcgc
    48661 caccagcccg ccgacccgtt ctgttttgcc gatattggcc cgctgctggc aacggcgctg
    48721 catgaccatc cgcgcctgcg tgagatgaac gtacagtttc cggcgcaaac cgtgcgcgcc
    48781 acggtgattg gcgcgggcgc gcataccctt tcgctctctg gcagcacaat ctggcttgag
    48841 ggcgtacaac tgccgctgcg caatttgccg gtggcgatcc cgattgatga aacggatctg
    48901 gtgagtgcct ggcaacaggc gctgcttcag ctggatcttg atcccaaaac tgacgcgtac
    48961 gtgctggcgc ttcccgcctc gctgcctgtg cgttacgccg cggtactgac ggtcatcaac
    49021 gcgctggtcg atttcgtcgc gcgttttcca aatccgcatc ccctgctggt ggtggccggg
    49081 caggactttg gtaaagctct gggcatgttg ttgcgcccac agctacaaca actcccgttg
    49141 gcagtcattg acgaagtgat tgtccgcgcg ggggactata tcgacattgg tacgcctctt
    49201 tttggcggat cggttgtgcc ggtgacggtg aaatcactcg catttccttc ctgagggaac
    49261 gacttatgaa actaaagacc acattgttcg gcaatgtata tcagtttaag gatgtaaaag
    49321 aggtgctggc taaagccaac gaactgcgtt cgggggatgt gctggcgggc gttgcagcgg
    49381 caagctcaca ggagcgcgtg gcggcaaagc aggtgttgtc ggaaatgacc gtagcggaca
    49441 tccgcaataa tccggtgatt gcctatgaag atgactgcgt gacgcggctg attcaggacg
    49501 atgttaacga aacggcctac aaccagatta aaaacaggag catcagcgaa ctgcgtgagt
    49561 atgtgctgag cgatgaaacc agcgtggacg acattgcctt tacccgcaaa gggctgacct
    49621 cggaagtggt cgcggcggta gcgaagattt gctccaacgc ggacctgatc tacggcgcga
    49681 agaaaatgcc ggtaatcaaa aaggccaata ccaccatcgg tattccgggc accttcagcg
    49741 cccgtttgca gccaaatgac acccgtgacg acgtgcaaag tatcgctgca caaatctacg
    49801 aagggctttc cttcggggtg ggcgatgcgg tgatcggcgt taacccggta actgacgacg
    49861 tggaaaactt aagccgcgtg ttggacacca tttacggcgt gatcgacaaa ttcaacatcc
    49921 caactcaggg ctgcgtactg gcgcacgtca ccacccagat cgaagcgatt cgtcgcggcg
    49981 cgcctggcgg actgattttc cagagtatct gtggcagcga aaaagggctg aaagagtttg
    50041 gcgtggaact ggcgatgctc gacgaagcgc gcgcagtggg tgctgagttc aaccgtatcg
    50101 ccggggaaaa ctgcctctac ttcgaaaccg ggcaaggttc ggcgctgtct gctggcgcta
    50161 acttcggcgc agactaggtg acgatggaag cacgtaacta tgggctggcg cgtcattacg
    50221 atccgtttat cgtcaacacc gtggtcggct ttattgggcc ggagtatctc tacaacgacc
    50281 gccagattat ccgtgctggc ttagaagatc actttatggg caagctgagc ggcatctcta
    50341 tgggctgtga ctgctgttac accaaccacg ctgatgctga ccagaacctc aacgaaaacc
    50401 tgatgatcct gctcgccacc gcaggctgca actacatcat ggggatgccg ctgggcgatg
    50461 acatcatgct caactatcag accaccgcat tccacgacac tgccactgtg cgtcagttac
    50521 tcaacctgcg cccgtcaccg gagtttgaac gctggctgga aagcatgggc attatggcaa
    50581 acggtcgcct gaccaaacgg gcgggcgatc cgtcactgtt cttctgatga cgcggagata
    50641 acacatcatg gatcaaaaac agattgaaga aattgtacgc agcgtgatgg cgtcaatggg
    50701 acaagcggcc cccgcgccgt cagaagcaaa gtgcgccacc actaactgtg cggcaccggt
    50761 gacctcggaa agctgcgcgc tggatttagg ttctgcggaa gcaaaagcgt ggattggtgt
    50821 tgaaaacccg catcgcgcag acgtattaac agaactgcgc cgcagcaccg tggcccgcgt
    50881 ttgtaccggt cgtaccggtc cgcgtccacg tacgcaggcg ctgctgcgtt tcctggccga
    50941 tcactctcgt tcgaaagata ccgtactgaa agaagtgccg gaagagtggg tgaaagcgca
    51001 gggcttgctg gaagtacgct ccgaaattag cgacaaaaat ctctacctga ctcgcccgga
    51061 tatgggccgc cgcctgtgtg cagaagctgt tgaagcgctg aaagcacagt gtgttgctaa
    51121 tccggacgta caggtcgtta tttctgatgg cctgtcaact gatgccatca ccgtgaacta
    51181 cgaagagatc ctgccgccgc tgatggcggg cctgaaacag gccgggctga aagtcggcac
    51241 gccattcttt gttcgttatg gtcgcgtgaa gattgaagat cagatcggtg agatcctcgg
    51301 cgcgaaagtg gtgatcctgc tggtggggga acgtccgggg ttgggtcagt cagaaagcct
    51361 ctcctgttac gccgtttact cgccgcgtat ggcgaccacc gttgaggccg atcgcacctg
    51421 catctctaac attcaccagg gcggcacgcc gccggttgaa gccgcagccg tcattgtgga
    51481 tttggcgaaa cgtatgctgg agcagaaagc atccggcatc aacatgaccc gataaggagg
    51541 catcatgcca gctttagatt tgattcgacc gtcggtaacc gctatgcggg tgattgcctc
    51601 cgttaactct gaatttgcgc gtgaactaaa attgccgccg catattcgta gcctcgggct
    51661 gatttctgct gattccgatg acgtcaccta cattgcggct gacgaagcga ccaagcaggc
    51721 gatggtcgaa gtggtatatg gccgctcgct gtatgcggga gcagcacacg gaccgtcacc
    51781 gactgccggt gaagtgctga ttatgcttgg tgggccaaac ccggcggaag tgcgtgctgg
    51841 tctggatgcg atggttgcgc atattgaaaa tggcgctgct ttccagtggg ctaatgacgc
    51901 agaaaacacc gcattcctgg cacatgtagt ttcgcgtacc ggttcttatc tttcatcaac
    51961 cgccgggatc acgcttggcg atccgatggc gtatctggtg gcaccgccgc tggaagcgac
    52021 ctacggcatt gatgcagcgt tgaaatctgc cgacgtacag ctggtgacct atgtcccgcc
    52081 accgtctgaa accaactact cggcagcatt tttaaccggt agccaggccg cgtgtaaagc
    52141 agcctgtaac gcctttaccg atgcagtgct ggaaatcgcg cgtaatccaa tccagcgtgc
    52201 gtaacggagg ttgccgatga tcaatgcact gggattgctg gaagtggacg gcatggtcgc
    52261 cgcgatagat gcagcggatg ccatgctcaa agcagctaac gttcgtctgc tcagtcacga
    52321 agtgcttgac cctggtcgct taacgctggt ggtggaaggc gatctggcgg cgtgtcgtgc
    52381 ggcgctggac gcaggttgtg ctgccgcgat gcgtaccggg cgtgtcatca gccgcaagga
    52441 gatcggtcgg ccagacgatg acacccagtg gctggttact ggctttaacc gccagccgaa
    52501 gcaacccgta aaggaacccg acgcgccagt tatcgtcgcg gaatctgctg acgagttgtt
    52561 ggcgctgtta acatcagtac gtcagggaat gacggcagga gaagtggctg cccactttgg
    52621 ctggccgctg gaaaaagcca gaaatgcgct cgaacagctc ttttctgccg ggacgttacg
    52681 taaacgcagt agtcgttatc gtctcaagcc ccattaacct gtcggaggtg ccgggtgtcc
    52741 tacaacaccc ggcattaaca tcatgaaaaa gacccgtaca gccaatttgc accatcttta
    52801 tcatgaaccc ttacccgaaa acctgaagct cacgccgaag gtcgaagtgg ataatgttca
    52861 tcaacgacag acaacggatg tctatgaaca tgctttgacg attaccgcct ggcagcagat
    52921 ttacgatcag ctgcatccgg gcaagtttca tggtgaattt acggaaattc tgctcgatga
    52981 tattcaggtt tttcgtgaat acaccggtct ggcgctgcgt cagtcgtgcc tggtctggcc
    53041 gaactcgttc tggtttggca ttccggcgac gcgcggtgag cagggattta tcggttcgca
    53101 atgtctggga agcgcagaaa tagcgacgcg ccccggtgga accgagtttg aattaagtac
    53161 gccggacgat tatacgatcc tgggcgtagt gctgtctgaa gatgtcatca cccgtcaggc
    53221 taactttttg cacaatccgg atcgggtatt acatatgctg cgtagccagt cggcgctgga
    53281 agtgaaagag cagcataaag ccgcgctgtg gggctttgtc caacaggcgc tggcgacgtt
    53341 ttgcgagaac ccggaaaatc tccatcagcc tgcagtgcga aaagtgctgg gggataattt
    53401 gctaatggcg atgggggcga tgctggaaga cgcgcaacca atggtgacgg cggaaagcat
    53461 cagtcatcag agttaccgta gattactttc ccgcgcccgt gaatatgtgc tggaaaatat
    53521 gtctgagctg gtgacggtgc tggacttgtg taatcaactg catgtcagtc gccgcacgct
    53581 acaaaacgcg tttcacgcta ttttaggcat tggcccaaac gcgtggctga aacgcattcg
    53641 cctgaacgcc gtacgccgcg aactgataag cccgtggtcg caaagcacaa cggtaaaaga
    53701 cgccgccatg cagtggggat tctggcatct ggggcaattt gccacggatt accagcagct
    53761 gtttgccgag aagccgtcgt tgacgttgca tcagcggatg cgggaatggg ggtgagggag
    53821 ttacacccaa tccctgacct taataaactc acttaacgcc gcttctgggc tgccatcttt
    53881 tggctgataa tcatactccc agcgtaccag tggcggcatt gacatcagga tcgactcggt
    53941 gcgcccgcca gtttgcaggc caaacagagt gccgcgatcc cagaccagat tgaactcgac
    54001 ataacgaccg cgacggtaga gctgaaaatt gcgctcgcgc tcgccgtagg ccatcgcttt
    54061 acgtcgctct acaattggta aataagcgtc ggtgtagcct ttgcctaccg cctgcataaa
    54121 ggcaaaacag tggtcgaaat ctggcgtgtt cagatcatca aagaacagcc cgccaatacc
    54181 gcgctgttcg ttgcgatgtt tgaggtagaa gtaatcgtcg caccactttt tgtaacgggg
    54241 ataaacgtct tcaccaaatg gcaggcacag gtcacgggcg gtgcggtgcc agtgaatggc
    54301 gtcttcttca aaaccataga aaggggttaa atcgaagccg ccgccaaacc accagacggg
    54361 ctctgcaccc ggtttttcgg caataaaaaa acgcacattc gcgtggctgg tgggaacata
    54421 cgggttatgc ggatgcacta ccagtgaaac gcccatcgcc tcgaaactgc gcccggcaag
    54481 ttccgggcga tgagcggtgg cggaagcagg catcgcctcg ccgtggacgt gagaaaagtt
    54541 gacgcctgcc tgttcgaaaa caccaccatt acgcaacacc cgactacgcc cgccgccgcc
    54601 agcttcgcgc tgccaactat cttcgacaaa ttctgcgcca tcgacggcgg tcagctgctg
    54661 acaaatcgta tcctgaaggt tgagcagaaa ctgtttaacc tggtgtgcgt cgggtttcat
    54721 aacttatcgc tttttcgaat gtgctttctg gttgtcgaac cagtggaaat aactgatcac
    54781 gccttcagca atcgctgtgg cgattttctg acgaaacgcc accgtgccta acagccgctc
    54841 ttcttccggg ttggtgataa acgaggtttc caccagcacc gaaggaaccg acggcgattt
    54901 caacaccaca aacgcggctt gttcggtgtt gcggctgtgc agtttatgca ccggcttaat
    54961 ctttttcaga atatgcgagc caagcgtcag gctgttttta atagtatcgg tttgcaccag
    55021 atcaaacagc acttgctgca ataggtgatc cttgtcagtc gcctttttac cggcaacttc
    55081 atcggcgcgg ttttcgcgtt cagacaggta tttcgccatt gcgctacttg ccccacggtt
    55141 agagagggca aataccgaag caccggcagc tttcgggttg gtaaagccat cggcatgaat
    55201 tgacataaac agatctgcgc catgtttatg ggcgatttca acgcgatcgt aaagtgggat
    55261 aaacgtatcg ccagaacgcg ttaaacgcgc atcaatccca tgattacgca aaatggaacg
    55321 gacgttttta gcaatcgcca gcaccacatg tttttctttc gaaccgttgc gtccgatcgc
    55381 tccggtatca attccgccgt ggcctggatc gagaacaacg acacgtttgc cgccagattt
    55441 tttggctttc ggcttgctgt gtccgttgct ggtttttaaa ggttcgtctt tggcgatggc
    55501 ttgcgacatt cctgacaacg tcagggcagc caaaccggct ttcagcacct ggcggcgcga
    55561 agtgagtgtt tttagtggtt taaaagtgct catacggcct gagttgtaat aataaagttc
    55621 cagatgttat atcgtatcgc gtaatccgtt acgacggttt gatgttgaga attgttttac
    55681 ttttcatttc aatagttgac aactccccat tatgccactt tttcaaccga ttttcgtcag
    55741 atattggcta attcgaccgt tcacgccata atcagccttt taacccgcaa aaaggtaacg
    55801 aataccatgg agatacgcgt atttcgccag gaagatttcg aagaggtcat caccctttgg
    55861 gagcgttgcg acttgctgcg tccgtggaac gatccggaaa tggacatcga gcgtaagatg
    55921 aaccatgacg tcagtttgtt tttggtcgct gaggtaaacg gtgaagtggt cggaacggtg
    55981 atgggcggtt atgacgggca tcgcgggtct gcttattatc ttggcgtgca tccagagttt
    56041 cgtgggcgtg ggattgccaa tgcgttgctt aatcggctgg agaaaaagct gattgctcgt
    56101 ggctgcccga aaattcagat caatgtgccg gaagataacg acatggtgct cggaatgtat
    56161 gaacgcctgg gatatgaaca cgccgacgtg ctgagtctgg gtaagcgttt gattgaagat
    56221 gaagagtact gagttccatc ctgtccatta tgatgcgcac ggacgcctgc gtttaccctt
    56281 gctcttctgg cttgtgctat tgcttcaggc gcgaacctgg gtgctgtttg tcattgccgg
    56341 tgcgtcgcgc gagcagggta ctgcgctgct aaatctgttt tatcccgatc acgataattt
    56401 ctggctgggg ctgattccag gcattccggc ggtgctggcg tttttgttga gcggtcggcg
    56461 ggctacattt cctcgcactt ggcatgtgct ctattttctg ttgctgttgg cgcaggtggt
    56521 tttactttgc tggcaaccgt ggctgtggct gaacggtgaa tccgttagcg gtatcggtct
    56581 ggcgctggta gtggcggata ttgtggcgtt aatctggctg ctgaccaatc gacgtttacg
    56641 cgcttgtttt aatgaagaaa aagaataacg gcactttttg gtgaatttgc actccaagca
    56701 acgttattga ataaccaaag gaagtgacat gaaatcgctg cgtttaatgt tatgcgctat
    56761 gccgttgatg ctgaccggct gttccacgat gtcgtcagtt aactggtcgg ccgctaaccc
    56821 gtggaactgg tttggatcgt ccaccaaagt gagcgagcag ggcgtgggtg aattaacggc
    56881 gtccacacca ctgcaagaac aagccattgc tgatgcgctt gatggcgatt atcgcctgcg
    56941 cagcggaatg aaaaccgcga acggcaacgt ggtgcgcttt tttgaagtga tgaaaggcga
    57001 caacgtggcg atggtgatta acggcgatca aggcacgatc agtcgcattg acgtgctgga
    57061 tagcgatatt cctgctgaca ccggtgttaa aatcggtaca ccgtttagcg acctttacag
    57121 caaagcattt ggcaattgcc aaaaagctga tggtgatgat aatcgtgccg tcgaatgtaa
    57181 agccgaaggc agtcaacata ttagctacca gttcagcggg gaatggcgtg gtcctgaagg
    57241 gttaatgcct tcggacgata ccctgaaaaa ctggaaagtc agtaaaatta tctggcggcg
    57301 ttaatttgcg tctgaacaaa ccgccgccgc gaaaaaacac gtaaaatagc gctcaacaat
    57361 gccacggatt gcgtggcatt cttattttca ggaggaacaa tgtctcaggt tcagagtggc
    57421 attttgccag aacattgccg cgcggcgatt tggatcgaag ccaacgtgaa aggggaagtt
    57481 gacgccctgc gtgcggccag taaaacattt gccgacaaac tggcaacttt tgaagcgaaa
    57541 ttcccggacg cgcatcttgg tgcggtggtt gcctttggta acaacacctg gcgcgctctg
    57601 agcggcggcg ttggggcaga agagctgaaa gattttccgg gctacggtaa aggccttgcg
    57661 ccgaccaccc agttcgatgt gttgatccac attctttctc tgcgtcacga cgtaaacttc
    57721 tctgtcgccc aggcggcgat ggaagccttt ggtgactgca ttgaagtgaa agaagagatc
    57781 cacggcttcc gttgggtgga agagcgtgac ctgagcggct ttgttgacgg tacagaaaac
    57841 ccggcgggtg aagagacgcg tcgcgaagtg gcggttatca aagacggcgt ggatgccggc
    57901 ggcagctatg tgtttgtgca gcgttgggag cacaacctga agcagctcaa ccggatgagc
    57961 gttcacgatc aggagatgat gatcgggcgc accaaagagg ccaacgaaga gattgacggc
    58021 gacgaacgtc cggaaacctc tcacctcacc cgcgttgatc tgaaagaaga tggcaaaggg
    58081 ctgaagattg ttcgccagag cctgccgtac ggcaccgcca gtggcactca cggtctgtac
    58141 ttctgcgcct actgcgcgcg tctgcataac attgagcagc aactgctgag catgtttggc
    58201 gataccgatg gtaagcgtga tgcgatgttg cgtttcacca aaccggtaac cggcggctat
    58261 tacttcgcgc cgtcgctgga caagttaatg gcgttgtaag cgtttctgat tcgatgatta
    58321 aaggccagcc agtaacaaaa ctgactggcc tttttattac ttttgcttca acgccgcata
    58381 cacctgatca actacccaac cataagttgc aatcggtaac tgaccgcttt cgaacatatt
    58441 gggatttttt tgtggatcgg caactggcga atggggcttg ttgcttaaca tcacaatcgc
    58501 catatgattg accggatcga taacggttac tgtcccggtc cagccagtgt gaccgtaggt
    58561 ttgcgggctt gccagcgtgc caaacgtcgg cgtcatggtg gcattaccat tcacgcgcca
    58621 gccgaggcca aaagtggcat cttccttaga gctggtggtg aacatcttca ccgtttccgc
    58681 actgaacagc tgcacatcgc catagccccc gccgttcagc atcgtttgca ttaacaccgc
    58741 aatatcgccg gtattggaaa acaaacctgc gtgcccggaa acgccgccca tcgaataaaa
    58801 ggctttttca tcgtgcacct gaccccagag agtggaggtg cggatattcg ggaaatggat
    58861 cacgccatcg cgggtattgc cgttcaattc cgtggcggca atttgttgcg gtttaaagcc
    58921 tttcagcagc gggttaaata ccgtatgtgt caggccgagc gggcgataaa tcgactcttc
    58981 aacatagcgg tcaagcggct gaccggtaac cgactcgacg ataaatccaa gcagcatata
    59041 atcgacatcg ctgtagatat gtttgctgcc gggctgatat tccagcggcg tgcgcttgat
    59101 catctccagc gtctggcctt tatcctggga atataacgcg cccgcgaccg ctttattcgg
    59161 gtattgcgga tctgccggga aaccgccgct gtgatgcagc aggtcagaaa tccgcagggt
    59221 gtttttgcct ttaatggtgt catttggact atcggcaaac cccggaatat atttcgcaat
    59281 ccgatcgtca ggatgcagtt tgccttcgct catcagcttt tgcagggcga agttcgtggc
    59341 gtacattttg gtgtttgagg ccaggtcata cagcgtcccg gtggtggctt tgacaggctg
    59401 ttccatcaac acgctgccat cgtacttttt cgccgctccc caggctttgc gatacacaat
    59461 ctgattatct ttaatgatca gcaggtttac gccgggataa ccgacatcaa tttgctggct
    59521 aatccagcga tccatctggt taagccgttc gacgttaaac cctgctttct ctggcgagct
    59581 ttctgtcaga acagggtatt ttgcggcgct gacgctacaa ctaacagcca gcagagaaag
    59641 atagagcatt gtccgtttca tgatatcagt ccagattgac gttacggcag ccaaagagcg
    59701 tggtgaaaat aaacccgcaa acccatgcca ccagaatccc gccagcataa accgccatgg
    59761 ccggaaggat gccttgtgcg gaagtcatca gcggcagcgc taccagacca gacggcccaa
    59821 aggcgctgtt taagcccatc ggcagacccc atcaggctat caggccgata aacaaaccgc
    59881 ccgccgcgcc gcctaaacag gcggtaacaa acggcttcat gcggggcagg gtgacaccgt
    59941 aaatcagcgg ttcaccaacg cccagcaggc cgggaataat cgccccgcgt acctgactgc
    60001 gtagcgcact gtgcggttgc gcccgccagt agagtgccag cgccgcgccc acctggcccg
    60061 cgcctgccat tgaaaggatg ggaaataagc tgttgaatcc ctagctgtcc attaacgcga
    60121 ggtaaacagg aataaagccc tgatgcacgc caaataccac ggcgatcagg aacagcccgg
    60181 ctaaaaccgc acaaccgaac ggattactgt tcaggtgcat aaacagccat gacatacctt
    60241 cgaataacca gccacccagc ggcataatga tcaggtacgc aagcgtagcg gtgatcagca
    60301 gggtgattaa cgaggtcagc agcatgtcga gatcgtccga cataaagcgg cgcaccatgc
    60361 cttcaatgcg ggcgcaggcc cacgcggcaa tcagcacgcc gataatattg ccgcgcggat
    60421 cgatgggcag accaaagaaa tcgtgaaaac cggcgtagtc accggtggtt gcagcggggt
    60481 tataaccgag caaaaagagt gcggcaataa ttgcgccatt gacgcccgtg ccgccaaatg
    60541 cctgggcggc gttatagccc accagaatca ccaggaaagt gaacaaacct ttgctgaaca
    60601 ccttcataaa attcagcgcg tcgggtagcg tgccctgagc atctgccgga acgtgcatca
    60661 ccgtggcaat taacgtcgct atccccagca gcagaccggc ggcaataaaa ccggggatca
    60721 gcggcgtgaa gatggtggcg aatttggcga gaaattgttg cacgccggag gtttgtttgg
    60781 cttttaactg acgtttgttc tgggcggcga tttctgcggc atcctgtacc ggtgcctcgc
    60841 ccagcagctc gctcatcgct tttgcggcac gatgcgcttt accaggccca aaaacgacct
    60901 gcacctggtc gctggtcaaa atgacgccct tcacgccttc cagcgttttg atattgggat
    60961 caaccagtga actgtcatgt acacccagac gcaggtgcgt catacagtta ccacaactgg
    61021 cgatatttcc cagtccgccg acacgggtaa gaatggtgtt cagaagttca ctgctgatct
    61081 ctttggccat gggttattcc ttgtctaaaa cctgacgaat aaagccgccg tgttgatcca
    61141 ggcgtttttt agcttctgcg gcatcgagat ttttcagcac catcacaatg gccgttttac
    61201 agttgcgctc gcaggcaatt aacgccgctt ccgcttgttc ggcattacat ccggtggcgt
    61261 ttttaacgat attgacctgg cgcacatgca gtttttcgtt ggtggcgacc acatcgacca
    61321 tcaggttgcc gaacactttg ccggatttaa tcatcagccc ggtggaaagc atattgagca
    61381 ccagtttctg cgctgtgcct gctttcattc gcgaagaacc agtgaccact tcggcaccta
    61441 caatcggtgt aatggcaaac tcggcggtgg tggaaacggc actccccgga ttacaggaaa
    61501 ttcccactgt gcggcaaccg agctggcggg cgtattccag tccggcaatc acatacggcg
    61561 tacgaccgct ggcggcaata ccgaccacca catcctgtgc cgttaaattt atatttttca
    61621 gatcgttaat gccgccttct cggctatctt ccgtgccttc caccgcgtgc tgaatggcat
    61681 attcgccgcc agcaatcaaa ccaaccacca gacccggttt cacgccgtag gtgggcggac
    61741 attcgctggc atccagaatc cccagacgac cggatgttcc cgcaccgaga taaatcagac
    61801 gaccgccgcc gcttacctgg gcgtggataa catcgatcgc cgcggcgata tccggcagga
    61861 cgcgctcaac ggcaagcggt acggttttat cttcatcgtt gataatccgg cacatttcca
    61921 gcgtcgatac gcggtcaatt tcagccgagg cggtgttcga gccttcagta atcatctttt
    61981 caagttgcat cggtggtgac cttactattt acgatcaaat aattgcagat gtatggaatc
    62041 atatatcccg aattgacaga cttaaaggaa tcattgagtc tacacttgca gagtgtgaag
    62101 agcgtcataa gaacttaagc ggaatttttg ctgccagccc gggagagtgg aagatgttgt
    62161 acctgacgaa aatacgcaat gcggaaagtg aatttaccga gaacgagcaa aaaattgctg
    62221 actttctgcg ggccaacgtg agtgagctga aatccgtctc ttcgcgcaag atggcgaaac
    62281 agctcggcat cagtcagtcg agcattgtga aatttgccca gaagctcggc gcgcaaggat
    62341 ttactgaatt gcgtatggcg ttaattggcg aatacagtgc cagccgggag aaaacgaatg
    62401 ccacggcgct gcacctgcac agttcgatta ccagtgatga ttcgctggaa gtgattgccc
    62461 gcaaactgaa tcgcgaaaaa gagctggcgc tggagcaaac ctgtgcgttg ttcgactacg
    62521 ctcggctgca aaaaatcatt gaggtcatca gtaaagcacc gtttatccag ataacgggcc
    62581 tgggcgggtc ggcgctggtg gggcgcgatc tgtcgttcaa actgatgaaa attggctatc
    62641 gcgtggccta tgaagccgac acacacgtac aagccaccgt ttcccaggcg ttgaaaaaag
    62701 gcgatgtaca aatcgctatc tcttacagcg gcagtaaaaa agagattgtc ttgtgcgcag
    62761 aagcggcacg aaagcagggg gcaacggtga ttgccattac ttctctggct gactctccgc
    62821 tgcggcggct ggcgcatttc acgctcgata ccgtttccgg tgaaaccgaa tggcgtagct
    62881 cgtcgatgtc tacccgcacg gcgcaaaatt ctgttaccga tttactgttt gttggtcttg
    62941 tgcaactcaa tgatgtggag tcattgaaaa tgattcagcg cagcagtgag ctaacacaac
    63001 gcctgaaatg atgcataaag cagcgactgg attgagattt tcctgaatta gtgagctgat
    63061 ccgcagcaat attttgttta tcctgtattt tcagagggaa tggagtgtaa cgctctgtat
    63121 taacaaggag agcattaaaa tgggtaaact cacgggcaag acagcactga ttacgggcgc
    63181 attgcaggga attggcgaag gaattgccag aacttttgcg cgtcatggcg caaacctaat
    63241 cttgctggat atctcccctg agatcgaaaa gctggcggac gaactgtgtg gtcgtggtca
    63301 tcgctgtacg gcggttgtcg ccgatgtgcg tgacccggcg tcggtagccg cagctatcaa
    63361 acgcgcgaag gaaaaagaag ggcgcattga tatcctggtg aataacgcag gcgtttgtcg
    63421 tctgggcagt ttcctcgata tgagcgatga cgatcgcgat ttccatattg acatcaatat
    63481 taaaggcgta tggaacgtca cgaaggcggt gctgccggag atgattgccc gcaaagatgg
    63541 tcgcattgtg atgatgtctt cagtcactgg tgatatggtg gccgatcctg gcgaaacggc
    63601 gtacgcctta acgaaagcgg cgattgttgg cctgacaaaa tcgctggcgg tggagtacgc
    63661 gcagtctggt attcgcgtta acgccatttg cccgggatac gtgcgcacac caatggcgga
    63721 aagcattgcc cgccagtcga acccggaaga tccagagtcg gtgctgactg aaatggcgaa
    63781 agcaatcccg ctgcgtcgcc tcgccgatcc gctggaagtc ggcgaactgg cggccttcct
    63841 cgcatcggat gaatccagct atttaaccgg tacacagaat gtgattgatg gcggcagcac
    63901 actgccggag acggttagcg tcggtatctg attcacctct gtttcctccc tgcatttgtg
    63961 gggaggattt cgttttaaac ccggttctta ttcccttttc aacttccaaa tcaccaaacg
    64021 gtatataaaa ccgttactcc tttcacgtcc gttataaata tgatggctat tagaaagtca
    64081 ttaaatttat aagggtgcgc aatggccgtt aacttactga aaaagaactc actcgcgctg
    64141 gtcgcttctc tgctgctggc gggccatgta caggcaacgg aactgctgaa cagttcttat
    64201 gacgtctccc gcgagctgtt tgccgccctg aacccgccgt ttgagcaaca atggaccaaa
    64261 gaaaacggcg gcgacaaact gacaataaaa caatctcatg ccgggtcatc aaaacaggcg
    64321 ctggcaattt tgcagggctt aaaagcagac gttgtcactt ataaccaggt gactgacgta
    64381 caaatcctgc atgacaaagg caagctgatc ccggcggact ggcaaacgcg cctgccaaat
    64441 aatagctcgc cattctactc caccatgggc ttcctggtgc gtaagggcaa cccgaagaat
    64501 atccacgact ggaacgacct ggtgcgctcc gatgtgaagc tgattttccc gaacccgaaa
    64561 acgtcgggta acgcgcgtta cacctatctg gcggcatggg gcgcagcgga taaagctgac
    64621 ggtggcgata aagccaaaac cgaacagttt atgacccagt tcctgaaaaa cgttgaagtg
    64681 ttcgatactg gcggtcgtgg cgcgaccacc acttttgccg agcgcggcct gggcgatgtg
    64741 ctgatcagct tcgagtcgga agtgaacaac atccgtaaac agtatgaagc gcagggtttt
    64801 gaagtggtga ttccgaaaac caacattctg gcggaattcc cggtggcgtg ggttgataaa
    64861 aacgtgcagg ccaacggtac ggaaaaagcc gccaaagcct accttaactg gctctacagc
    64921 ccgcaggcgc aaaccatcat caccgactat tactaccgcg tgaataaccc ggaagtcatg
    64981 gataaactga aagacaaatt cccgcagacc gagctgttcc gcgtggaaga caaatttggc
    65041 tcctggccgg aagtgatgaa aacccacttc accagcggcg gcgagttaga caagctgtta
    65101 gcggcggggc gtaattaatg tttgcagttt catcaagacg cgtgctgccg ggctttacct
    65161 taagcctcgg caccagtctg ctgtttgtgt gcctgatttt gctgctgccg ctctccgcgc
    65221 tggtgatgca gctggctcag atgagctggg cgcagtactg ggaggtgatc accaacccgc
    65281 aggtagtagc ggcctacaaa gtaacgctgc tgtcagcgtt tgtggcatcg atttttaacg
    65341 gcgtttttgg cctgctgatg gcgtggatct taacccgcta tcgctttccg ggccgcacgc
    65401 tgcttgatgc gctgatggat ttaccctttg cgctgccaac ggcagtcgcc ggtttaacgc
    65461 tagcctcgct cttttctgta aacggttttt acggtgaatg gctggcgaag tttgatatca
    65521 aagtcaccta tacctggctg gggattgcgg tggcgatggc ctttaccagc attccgtttg
    65581 tggtacgtac cgtgcagccg gtgctggaag agttaggccc ggaatatgaa gaagcggcag
    65641 aaacgcttgg cgcaacgcgc tggcagagtt tctgcaaagt ggtgctgccg gaactttctc
    65701 cggcgctggt ggcgggcgtg gcgctgtcgt ttacccgtag tcttggtgaa tttggcgcgg
    65761 tgatttttat cgccggaaat atcgcgtgga agacggaagt gacgtcgctg atgatttttg
    65821 tgcgcttaca ggagtttgat tacccggcag cgagcgcgat tgcttcggtg atcctcgcgg
    65881 catctctgct gctgctgttc tcaattaaca ctctgcaaag tcgctttggt cggcgtgtgg
    65941 taggtcatta atggcggaag ttacccaatt gaagcgttat gacgcgcgcc cgattaactg
    66001 gggcaaatgg tttctgattg gcatcgggat gctggtatcg gcgttcatcc tgctggtgcc
    66061 gatgatttat atcttcgtac aggcgttcag caaagggctg atgccggttt tacagaatct
    66121 ggccgatccg gacatgctgc acgccatctg gctgacggtg atgatcgcgc tgattgccgt
    66181 accggtaaac ctggtgttcg gcattctgct ggcctggctg gtgacgcgct ttaacttccc
    66241 tggacgccag ttactgctga cgctactgga cattccgttt gccgtatcgc cggtggttgc
    66301 cggtctggtg tatctgctgt tctacggctc taacggcccg ctgggcggct ggctggatga
    66361 acataatctg caaattatgt tctcctggcc cggaatggtg ctggtcacca tctttgtgac
    66421 gtgtccgttt gtggtgcgcg agctggtgcc ggtgatgtta agccagggca gccaggaaga
    66481 cgaagcggcg attttgcttg gcgcgtctgg ctggcagatg tttcgccgcg tcacattgcc
    66541 gaacattcgc tgggcgctgc tttatggcgt ggtgttgacc aacgcccgcg caattggcga
    66601 gtttggcgcg gtgtcggtgg tttccggctc gattcgcggc gaaaccctgt cgctgccgtt
    66661 acagattgaa ttgctggagc aggactacaa caccgtcggc tcctttaccg ctgcggcact
    66721 gttaacgctg atggcgatta tcaccctgtt tttaaaaagt atgttgcagt ggcgcctgga
    66781 gaatcaggaa aaacgcgcac agcaggagga acatcatgag cattgagatt gccaatatta
    66841 agaagtcgtt tggtcgcacc caggtgctga acgatatctc actggatatt ccttcaggtc
    66901 agatggtcgc gttgctgggg ccgtccggtt ccgggaaaac cacgctgctg cgcattatcg
    66961 ccgggctgga gcatcaaacc agcgggcata ttcgcttcca cggcaccgac gtcagccgcc
    67021 tgcacgcgcg cgatcgcaag gtcggtttcg tgttccagca ttacgcgctg ttccgccata
    67081 tgacggtatt cgacaatatc gcttttggcc tgacggtgct gccgcgtcgc gatcgcccga
    67141 atgccgcagc catcaaagcg aaagtgacaa aattgctgga gatggtgcag cttgctcatc
    67201 tggcggatcg ttatccggca cagctttccg gcggccagaa acagcgcgtg gcgctggcgc
    67261 gcgcgctggc tgtagaaccg caaattctgc tgcttgatga accgtttggc gcgctggatg
    67321 cgcaggtgcg taaagagctg cgtcgctggc tgcgtcaact gcatgaagag ctgaaattca
    67381 ccagcgtgtt cgtgacccac gaccaggaag aagcgacgga agtagctgat cgtgtagtgg
    67441 tgatgagcca gggcaatatt gaacaggctg atgcgccgga gcaggtatgg cgcgaaccag
    67501 cgacccgttt tgtgctcgaa tttatgggcg aagtgaaccg cctgcaggga accattcgcg
    67561 gcgggcagtt ccatgttggc gcacatcgct ggccgctggg ctacacacct gcttatcagg
    67621 ggccggtgga tctcttcctg cgcccgtggg aagtggatat cagccgccgt accagtctcg
    67681 attcgccgct gccggtacag gtactggaag ccagcccgaa aggtcactac acccaattag
    67741 tggtgcagcc gctggggtgg tacaacgaac cgctgacggt cgtgatgcat ggcgacgatg
    67801 ccccgcagcg tggcgagcgt ttattcgttg gtctgcaaca tgcgcggctg tataacggcg
    67861 acgagcgtat cgaaacccgc gatgaggaac ttgctctcgc acaaagcgcc tgataggttg
    67921 agtgaatgtt aaacgcccgg aggcgcttcc cgcgatccgg gctttttaat ggcaaggttt
    67981 gtaacctgta gacctgataa gacgcgcaag cgtcgcatca ggcaacacca cgtatggata
    68041 gagatcgtga gtacattaga acaaacaata ggcaatacgc ctctggtgaa gttgcagcga
    68101 atggggccgg ataacggcag tgaagtgtgg ttaaaactgg aaggcaataa cccggcaggt
    68161 tcggtgaaag atcgtgcggc actttcgatg atcgtcgagg cggaaaagcg cggggaaatt
    68221 aaaccgggtg atgtcttaat cgaagccacc agtggtaaca ccggcattgc gctggcaatg
    68281 attgccgcgc tgaaaggcta tcgcatgaaa ttgctgatgc ccgacaacat gagccaggaa
    68341 cgccgtgcgg cgatgcgtgc ttatggtgcg gaactgattc ttgtcaccaa agagcagggc
    68401 atggaaggtg cgcgcgatct ggcgctggag atggcgaatc gtggcgaagg aaagctgctc
    68461 gatcagttca ataatcccga taacccttac gcccattaca ccactaccgg gccggaaatc
    68521 tggcagcaaa ccggcgggcg cattacccat tttgtctcca gcatgggaac aaccggcact
    68581 atcaccggcg tttcacgctt tatgcgcgaa caatccaaac cagtaacgat tgttggcctg
    68641 cagccggaag aaggcagcag tattcccggc attcgccgct ggcctgcgga atatctgccg
    68701 gggattttca acgcttctct ggtggatgag gtgctggata ttcatcagcg cgatgcagaa
    68761 aacaccatgc gcgaactggc agtgcgggaa ggaatattct gtggcgtcag ctccggcggc
    68821 gcggttgccg gagcactgcg ggtggcaaaa gctaaccctg gtgcggtggt ggtagcgatc
    68881 atctgcgatc gtggcgatcg ctacctttcc accggggtgt ttggggaaga gcattttagt
    68941 cagggggcgg ggatttaaga ttattgcgca ggcaccgttt tatccgtctg cgcaattaag
    69001 gcatgtaaaa aaggctgtgc gttctcatcg ctcttaccgg ggatcttcac aatatacggc
    69061 ttcccggtaa tcgatgacga cgaagcaacc ttatcaataa actgctcggc agtgtcaatg
    69121 cggttacggg tattgccgag cttcagacgc agatgagaaa ccgcttcatc gcaggtatgt
    69181 tcatcaccgt tgcgcacaaa tatcaaatcc tttttctgtg ctaatccctc cagcatggcg
    69241 ttgatgcggg cttcttcatg aacagttaac ttcgcgtaag cggggagtgt cattagtagt
    69301 gtaatgacca gacaaatgat tttcttcatt gaattacctt atccttgttt tgtgatagcc
    69361 gatttgactg ccagaatcag gtaaagtttc ggttggaata cgatgaggat catcggcaag
    69421 gagaaaaaat taaatgagta gtttgttgtt gtttaacgat aagagtaggg cgctgcaggc
    69481 ggatatcgtc gccgtgcagt cgcaggtagt gtacggcagc gtaggcaaca gcattgcggt
    69541 gcctgctata aaacagaacg gcttgaatgt ctttgccgtg ccgacggtat tgctgagcaa
    69601 tacgccgcat tatgacactt tctacggtgg tgcgattccg gacgaatggt ttagcggcta
    69661 tttgcgtgcg cttcaggagc gtgatgcgct gcgccaactt cgtgctgtaa ccacgggcta
    69721 tatgggaacg gcatcgcaaa tcaaaatcct tgccgagtgg ctgactgcgc tacgcaaaga
    69781 ccatcctgac ctattgatca tggtcgatcc ggtgattggc gatattgata gcggaattta
    69841 tgtcaaacct gaccttcccg aggcgtatcg acaatattta ctgccgctgg cgcaaggaat
    69901 tacccccaat atctttgagc tggaaatcct gaccggtaaa aattgccgcg atctcgacag
    69961 tgccattgct gccgcaaaaa gtctgctttc agacacatta aaatgggtgg tgattaccag
    70021 cgcctccggt aatgaagaaa atcaggagat gcaggtggtg gtggtcagtg ccgacagcgt
    70081 gaatgtcatt tcccattcac gggtaaaaac cgacctgaaa gggactggcg acctgttttg
    70141 tgctcagctt atcagtggtt tgctgaaagg gaaggcgtta aacgatgcgg tgcaccgtgc
    70201 ggggttgcgc gtactggaag tgatgcgcta tacccagcag catgagagcg atgaattgat
    70261 tttgccgccg ctggcggaag cataaaaaat ggcgccgatg g
//
LOCUS       DAZPAC010000002        57269 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZPAC010000002 DAZPAC010000000
VERSION     DAZPAC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57269
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.002"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(108..1037)
                     /locus_tag="ACHUPM_000068"
     CDS             complement(108..1037)
                     /locus_tag="ACHUPM_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0867975.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(1083..1907)
                     /locus_tag="ACHUPM_000069"
     CDS             complement(1083..1907)
                     /locus_tag="ACHUPM_000069"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0867976.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(1990..2244)
                     /gene="yoeB"
                     /locus_tag="ACHUPM_000070"
     CDS             complement(1990..2244)
                     /gene="yoeB"
                     /locus_tag="ACHUPM_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR0867977.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(2241..2492)
                     /gene="yefM"
                     /locus_tag="ACHUPM_000071"
     CDS             complement(2241..2492)
                     /gene="yefM"
                     /locus_tag="ACHUPM_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR0867978.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            2775..2825
                     /gene="hisL"
                     /locus_tag="ACHUPM_000072"
     CDS             2775..2825
                     /gene="hisL"
                     /locus_tag="ACHUPM_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFR0867979.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    2801..2924
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            2971..3870
                     /gene="hisG"
                     /locus_tag="ACHUPM_000073"
     CDS             2971..3870
                     /gene="hisG"
                     /locus_tag="ACHUPM_000073"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR0867980.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            3876..5180
                     /gene="hisD"
                     /locus_tag="ACHUPM_000074"
     CDS             3876..5180
                     /gene="hisD"
                     /locus_tag="ACHUPM_000074"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR0867981.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            5177..6247
                     /gene="hisC"
                     /locus_tag="ACHUPM_000075"
     CDS             5177..6247
                     /gene="hisC"
                     /locus_tag="ACHUPM_000075"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR0867982.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCESGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            6247..7314
                     /gene="hisB"
                     /locus_tag="ACHUPM_000076"
     CDS             6247..7314
                     /gene="hisB"
                     /locus_tag="ACHUPM_000076"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR0867983.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            7314..7904
                     /gene="hisH"
                     /locus_tag="ACHUPM_000077"
     CDS             7314..7904
                     /gene="hisH"
                     /locus_tag="ACHUPM_000077"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR0867984.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            7904..8641
                     /gene="hisA"
                     /locus_tag="ACHUPM_000078"
     CDS             7904..8641
                     /gene="hisA"
                     /locus_tag="ACHUPM_000078"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR0867985.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            8623..9399
                     /gene="hisF"
                     /locus_tag="ACHUPM_000079"
     CDS             8623..9399
                     /gene="hisF"
                     /locus_tag="ACHUPM_000079"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR0867986.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            9393..10004
                     /gene="hisIE"
                     /locus_tag="ACHUPM_000080"
     CDS             9393..10004
                     /gene="hisIE"
                     /locus_tag="ACHUPM_000080"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR0867987.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(10101..11078)
                     /gene="wzzB"
                     /locus_tag="ACHUPM_000081"
     CDS             complement(10101..11078)
                     /gene="wzzB"
                     /locus_tag="ACHUPM_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR0867988.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLAQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(11224..12390)
                     /gene="ugd"
                     /locus_tag="ACHUPM_000082"
     CDS             complement(11224..12390)
                     /gene="ugd"
                     /locus_tag="ACHUPM_000082"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFR0867989.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(12640..14046)
                     /gene="gndA"
                     /locus_tag="ACHUPM_000083"
     CDS             complement(12640..14046)
                     /gene="gndA"
                     /locus_tag="ACHUPM_000083"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR0867990.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(14210..15580)
                     /gene="cpsG"
                     /locus_tag="ACHUPM_000084"
                     /gene_synonym="manB"
     CDS             complement(14210..15580)
                     /gene="cpsG"
                     /locus_tag="ACHUPM_000084"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR0867991.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(15685..17121)
                     /gene="cpsB"
                     /locus_tag="ACHUPM_000085"
     CDS             complement(15685..17121)
                     /gene="cpsB"
                     /locus_tag="ACHUPM_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR0867992.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(17124..18347)
                     /gene="wcaI"
                     /locus_tag="ACHUPM_000086"
     CDS             complement(17124..18347)
                     /gene="wcaI"
                     /locus_tag="ACHUPM_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR0867993.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(18344..18823)
                     /gene="gmm"
                     /locus_tag="ACHUPM_000087"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(18344..18823)
                     /gene="gmm"
                     /locus_tag="ACHUPM_000087"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR0867994.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(18826..19791)
                     /gene="fcl"
                     /locus_tag="ACHUPM_000088"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(18826..19791)
                     /gene="fcl"
                     /locus_tag="ACHUPM_000088"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR0867995.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(19794..20915)
                     /gene="gmd"
                     /locus_tag="ACHUPM_000089"
     CDS             complement(19794..20915)
                     /gene="gmd"
                     /locus_tag="ACHUPM_000089"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR0867996.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(20941..21489)
                     /gene="wcaF"
                     /locus_tag="ACHUPM_000090"
     CDS             complement(20941..21489)
                     /gene="wcaF"
                     /locus_tag="ACHUPM_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR0867997.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(21505..22251)
                     /gene="wcaE"
                     /locus_tag="ACHUPM_000091"
     CDS             complement(21505..22251)
                     /gene="wcaE"
                     /locus_tag="ACHUPM_000091"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR0867998.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(22262..23479)
                     /gene="wcaD"
                     /locus_tag="ACHUPM_000092"
     CDS             complement(22262..23479)
                     /gene="wcaD"
                     /locus_tag="ACHUPM_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFR0867999.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(23454..24671)
                     /gene="wcaC"
                     /locus_tag="ACHUPM_000093"
     CDS             complement(23454..24671)
                     /gene="wcaC"
                     /locus_tag="ACHUPM_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR0868000.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(24668..25156)
                     /gene="wcaB"
                     /locus_tag="ACHUPM_000094"
     CDS             complement(24668..25156)
                     /gene="wcaB"
                     /locus_tag="ACHUPM_000094"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR0868001.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(25159..25998)
                     /gene="wcaA"
                     /locus_tag="ACHUPM_000095"
     CDS             complement(25159..25998)
                     /gene="wcaA"
                     /locus_tag="ACHUPM_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFR0868002.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(26091..28253)
                     /gene="wzc"
                     /locus_tag="ACHUPM_000096"
     CDS             complement(26091..28253)
                     /gene="wzc"
                     /locus_tag="ACHUPM_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR0868003.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(28256..28699)
                     /gene="wzb"
                     /locus_tag="ACHUPM_000097"
     CDS             complement(28256..28699)
                     /gene="wzb"
                     /locus_tag="ACHUPM_000097"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR0868004.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(28705..29844)
                     /gene="wza"
                     /locus_tag="ACHUPM_000098"
     CDS             complement(28705..29844)
                     /gene="wza"
                     /locus_tag="ACHUPM_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR0868005.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            30153..30302
                     /locus_tag="ACHUPM_000099"
     CDS             30153..30302
                     /locus_tag="ACHUPM_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868006.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            30503..32086
                     /locus_tag="ACHUPM_000100"
     CDS             30503..32086
                     /locus_tag="ACHUPM_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0868007.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(32360..34213)
                     /gene="asmA"
                     /locus_tag="ACHUPM_000101"
     CDS             complement(32360..34213)
                     /gene="asmA"
                     /locus_tag="ACHUPM_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR0868008.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(34235..34816)
                     /gene="dcd"
                     /locus_tag="ACHUPM_000102"
     CDS             complement(34235..34816)
                     /gene="dcd"
                     /locus_tag="ACHUPM_000102"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR0868009.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(34908..35549)
                     /gene="udk"
                     /locus_tag="ACHUPM_000103"
     CDS             complement(34908..35549)
                     /gene="udk"
                     /locus_tag="ACHUPM_000103"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR0868010.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            35867..39184
                     /locus_tag="ACHUPM_000104"
     CDS             35867..39184
                     /locus_tag="ACHUPM_000104"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0868011.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(39294..40142)
                     /gene="alkA"
                     /locus_tag="ACHUPM_000105"
     CDS             complement(39294..40142)
                     /gene="alkA"
                     /locus_tag="ACHUPM_000105"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFR0868012.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            40276..41628
                     /gene="yegD"
                     /locus_tag="ACHUPM_000106"
     CDS             40276..41628
                     /gene="yegD"
                     /locus_tag="ACHUPM_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0868013.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(41641..43581)
                     /gene="yegI"
                     /locus_tag="ACHUPM_000107"
     CDS             complement(41641..43581)
                     /gene="yegI"
                     /locus_tag="ACHUPM_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFR0868014.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(43578..44339)
                     /gene="pphC"
                     /locus_tag="ACHUPM_000108"
     CDS             complement(43578..44339)
                     /gene="pphC"
                     /locus_tag="ACHUPM_000108"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFR0868015.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(44336..44995)
                     /locus_tag="ACHUPM_000109"
     CDS             complement(44336..44995)
                     /locus_tag="ACHUPM_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR0868016.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(45216..45275)
                     /gene="ibsA"
                     /locus_tag="ACHUPM_000110"
     CDS             complement(45216..45275)
                     /gene="ibsA"
                     /locus_tag="ACHUPM_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFR0868017.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(45548..45604)
                     /locus_tag="ACHUPM_000111"
     CDS             complement(45548..45604)
                     /locus_tag="ACHUPM_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0868018.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(45875..45931)
                     /locus_tag="ACHUPM_000112"
     CDS             complement(45875..45931)
                     /locus_tag="ACHUPM_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0868019.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            46210..47451
                     /gene="mdtA"
                     /locus_tag="ACHUPM_000113"
     CDS             46210..47451
                     /gene="mdtA"
                     /locus_tag="ACHUPM_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFR0868020.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            47451..50573
                     /gene="mdtB"
                     /locus_tag="ACHUPM_000114"
     CDS             47451..50573
                     /gene="mdtB"
                     /locus_tag="ACHUPM_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFR0868021.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            50574..53651
                     /gene="mdtC"
                     /locus_tag="ACHUPM_000115"
     CDS             50574..53651
                     /gene="mdtC"
                     /locus_tag="ACHUPM_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR0868022.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            53652..55067
                     /locus_tag="ACHUPM_000116"
     CDS             53652..55067
                     /locus_tag="ACHUPM_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0868023.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            55064..56467
                     /gene="baeS"
                     /locus_tag="ACHUPM_000117"
     CDS             55064..56467
                     /gene="baeS"
                     /locus_tag="ACHUPM_000117"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR0868024.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            56464..57186
                     /gene="baeR"
                     /locus_tag="ACHUPM_000118"
     CDS             56464..57186
                     /gene="baeR"
                     /locus_tag="ACHUPM_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR0868025.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        13358 a        15253 c        14811 g        13847 t
ORIGIN      
        1 gacgctgatg aaacgtcggt ttttagtcat tttttgacag cggcgcattg tgcctaaatg
       61 ggggggaaat gacaagagaa tgagaagctt gtcataataa tttttcttta aatggctgat
      121 tttccgtcat cagattttgc ataaacaccg gcgatcgcac tatttgctaa aatttcatcc
      181 cgccaccagc cgctggcaag ccctgcggtc tgttcgttcc agccaatcca gacgggttca
      241 aagagagttt gggcgaccac tttcttctcg attaacgcgc cactatcgag aaaacgttgc
      301 accagataac ggggtaaata gccgcagccg aggccgctaa tttgcagctc cagcttggtt
      361 ttaaaatcga aaacggtaat ggcttcttgt tcgtcgagaa gttgtgaggc cgtagaagcg
      421 cctgcctgcg cagtgtcccc caccacaatc gcccgatatc gcttaatgat acgacggttt
      481 aacggctctt cttcctgggc taacggatga tgaggcgcga ccgcaaagac ttgttccaga
      541 tcgcccaacc gcgagaaacc aaactcactg gaagatggtg gctcatgcat cgcgccaaca
      601 ataatatccg ctcgcccctg agtcagggca tcccaggaac caccgagtac gccattgata
      661 aacttcaggc gcgtcacgct atgatgttga taaaaagctt caatcagtgg cgcaagcaga
      721 gaaaaaggaa acgtgtcgtc cacgccaatc accagctcgt tttcccagcc ttcatgcagt
      781 ttgatcgcct gcttttccag ttctcgcacg gtatgcagaa cttcccgccc cttctctaat
      841 aacatttttc cggtacgggt gaatttagcg cggtgaccgc tacgatcaag taattgaata
      901 ttaaggtcgc tttccagctt gtgaacggtg taactcagag cggacggcgt cttgtagagt
      961 ttagccgacg ccgccgcaaa actgccttct ttttctaagg catcgaggat catcaacaca
     1021 tccagcagtt gtttcataca cgcccccttg cagtgcatgc ggcgtcctgc tggcgtgatc
     1081 gtttactcca gcggcattac cagcggatcg ggatactggt attcaaatcc cagttcatta
     1141 caaatccgac tgccatcaat aatcttacct ttgccgctgt ccagactatt tctgaactgc
     1201 ggaggttcca gccccagtaa acgggccatc tgcggataga aaacattacg cgcagggtga
     1261 gctggcgcac atatattata gatgtgtccg cctttaggtg cctgtaacaa cagagtgata
     1321 gcgccaataa catcttctaa atggactaaa ttaacaccat gttcaccatc aggcgcggtt
     1381 tttccggcaa agaagcgtcc gggatgacgt cccggtccga ccagccccgc aagacgcaga
     1441 atatcgaccg aagtaccggg taaattgtgc agccagtctt cgagttcttc taatactcgt
     1501 ccactgttgg tcaccggatt acgcggggtg gtttctttca ccgtgccttg cgcgtcgcca
     1561 tagacagacg ttgagctggt aaaaataatg cgaggaatac gatgggccag cgcgctatcc
     1621 actaactctt gtaccgcttg taaatagaac tcatcgccgg ggccgctacg acgtgccgga
     1681 agcgtaatga ccagcgcatc ggcatccatc agggcatcca gatcgtcaga atcgcaaact
     1741 aactcaggct ccatgcgaag cagatagcta tcaatgccac tcattcgggc cgcttcgaca
     1801 ccatcttgtg tggttttact cccggtgact tgccagcctc gcgctgaaag tgacatcgcc
     1861 agcggcatgc ctaaccaccc taacccgaca attgcgacct ttttcatccg ttatctcctg
     1921 atacacctta gatctataag gctacgctag cgtatcaaaa ctgacaattc attctatgaa
     1981 tgaatctgtt caataatgat aacgacatgc tgcaatgagc agtgaatcgt cggtaaccgc
     2041 gtataccaga cggtgctcct ctgtaatgcg tcgggaccag aaacctgaca aattatgttt
     2101 caggggttct ggcttcccct taccttcaaa tggcgttctg cgggtatctt tgataagttc
     2161 attgatcttt ttaacaattc gcttatctgt ttcctgccag tacagataat catcccatga
     2221 ttcctcagac cagattagtt tcactcaata atgtcctttt ctgttccttt gcctgatttc
     2281 aggctatcga ttgagtccat caatctccgg gcgttagcgg gggagcgcag tagataagcc
     2341 gtctcttcca gcgagttgta ttcttcgagt gacatcagaa cacaagcctc tccattctga
     2401 cgagtaataa ggatcggggc atgatcttca acggctttca tcattgttgc cgacaaattc
     2461 tgacgcgctt cgctgtagct aattgtacgc atgtcaatct cctcttttgt acagtttatt
     2521 gtacaatgat gggcgttaat taactattta ttaattagtt tgtagatcaa ggtattgtca
     2581 gtgagacgaa aatccaggct tcgctatttt tggtgccgtc agcttagagg acagtcctct
     2641 tagccccctc ctttccccgc tcattcatta aacaaatcca ttgccataaa atatataaaa
     2701 aagcccttgc tttctaacgt gaaagtggtt taggttaaaa gacatcagtt gaataaacat
     2761 tcacagagac ttttatgaca cgcgttcaat ttaaacacca ccatcatcac catcatcctg
     2821 actagtcttt caggcgatgt gtgctggaag acattcagat cttccagtgg tgcatgaacg
     2881 catgagaaag cccccggaag atcaccttcc gggggctttt ttattgcgcg gttgataacg
     2941 gttcagacag gtttaaagag gaataacaaa atgacagaca acactcgttt acgcatagct
     3001 atgcagaaat ccggccgttt aagtgatgac tcacgcgaat tactggcgcg ctgtggcatt
     3061 aaaattaatc ttcacaccca gcgcctgatc gcgatggcag aaaacatgcc gattgatatt
     3121 ctgcgcgtgc gtgacgacga cattcccggt ctggtaatgg atggcgtggt agaccttggg
     3181 attatcggcg aaaacgtgct ggaagaagag ctgcttaacc gccgcgccca gggtgaagat
     3241 ccgcgctact ttaccttgcg tcgtctggat ttcggcggct gccgcctttc gctggcaacg
     3301 ccggttgatg aagcctggga cggcccgctc tccttaaacg gtaaacgtat cgccacctct
     3361 tatcctcacc tgctcaagcg ttatctcgac cagaaaggca tctcttttaa atcctgctta
     3421 ctgaacggtt ctgttgaagt cgccccgcgt gccggactgg cggatgcgat ttgcgatctg
     3481 gtttccaccg gtgccacgct ggaagcaaac ggcctgcgcg aagtcgaagt tatctaccgc
     3541 tcgaaagcct gccttatcca gcgcgatggt gaaatggaag aatccaaaca gcagctgatc
     3601 gacaaactgc tgacccgtat tcagggtgtg atccaggcgc gcgaatcaaa atacatcatg
     3661 atgcacgcac cgaccgaacg tctggatgaa gtcatcgccc tgctgccagg tgccgaacgc
     3721 ccaaccattc tgccgctggc gggtgaccaa caacgcgtag cgatgcacat ggtcagtagc
     3781 gaaaccctgt tctgggaaac gatggaaaaa ctgaaagcgc tgggtgccag ttcaattctg
     3841 gtcctgccga ttgagaagat gatggagtga tcgccatgag ctttaacaca atcattgact
     3901 ggaatagctg tactgcggag caacaacgcc agctgttaat gcgcccggcg atctccgctt
     3961 ctgaaagcat tacccgcact gttaacgata ttctcgataa cgtgaaagca cgcggtgatg
     4021 acgccctgcg ggaatacagc gcgaagtttg ataaaaccac ggttaacgcg ctgaaggtgt
     4081 ctgctgagga gattgccgcc gctagcgaac gcctgagcga cgagctaaaa caggcgatgg
     4141 cggtggcagt aaaaaatatt gaaaccttcc acactgcgca aaaactgccg ccggtagatg
     4201 tagaaacgca gccaggcgtg cgttgccagc aggtcacgcg cccggtcgct tcagttgggt
     4261 tgtatattcc tggcggctcc gccccgctct tctcaacggt attaatgctg gcgactccgg
     4321 cgcgtattgc gggctgtaaa aaagtggtgc tgtgctcacc gccgccgatt gccgatgaga
     4381 tcctttatgc ggcgcagctg tgcggtgtga aggacgtgtt taacgtcggc ggcgcacagg
     4441 ccattgccgc gctggcgttt ggtacggaat ctgtgccgaa agtggacaaa atcttcgggc
     4501 cgggtaacgc ctttgtcacc gaagcgaaac gtcaggtgag ccagcgtctg gacggtgcgg
     4561 ctatcgatat gcccgcaggc ccgtcggaag tgttggtcat tgccgacagc ggcgcaacgc
     4621 cggatttcgt ggcttctgat ttgctttctc aggctgaaca cggcccggac tcacaggtga
     4681 ttttactgac gcctgacgct gatatggcgc atcaagttgc cgaagccgtc gaacgccagt
     4741 tagcagaact gccgcgtgcc gaaaccgccc gccaggcact gaacgccagc cgcctgatcg
     4801 tgactaaaga tttagcgcag tgcgtagaga tctccaacca gtacggcccg gagcacctga
     4861 tcatccagac ccgcaacgcc cgcgatctgg tcgatggcat caccagcgcc ggttcggtat
     4921 ttcttggtga ctggtcaccg gaatcggcag gtgattacgc ctccggcacc aaccacgttc
     4981 tgccgactta cggttacacc gccacctgtt ccagcctcgg gctggcagat ttccagaagc
     5041 gtatgaccgt gcaggaactg tcgaaagaag gtttctccgc gctggcttcg accattgaaa
     5101 cattggccgc cgccgagcgc ctgaccgccc ataaaaatgc cgttactttg cgtgttaacg
     5161 cccttaagga gcaagcatga gcaccgtgac tattaccgat ttagcgcgtg aaaacgtccg
     5221 caacctgacg ccgtatcagt cggcgcgtcg tctgggcggt aatggcgacg tctggctgaa
     5281 cgccaacgaa taccccactg ccgtggagtt tcagcttact cagcaaacgc tcaaccgcta
     5341 cccggaatgt cagccgaaag cggtgattga aaattacgcg caatatgcag gcgtaaaacc
     5401 ggaacaggtg ctggtcagtc gtggcgcgga cgaaggtatt gaactgctga ttcgcgcttt
     5461 ttgcgaatcg ggtaaagacg ccatcctcta ctgcccgcca acgtacggca tgtacagcgt
     5521 cagcgccgaa acgattggcg tcgagtgccg cacagtgccg acgcaggaca actggcaact
     5581 ggacttacag ggcatttccg acaagctgga cggcgtaaaa gtggtctatg tttgcagccc
     5641 caacaacccg acagggcaac tgatcaatcc gcaggatttt cgcactctgc tggagctaac
     5701 ccgcggaaaa gccattgtgg ttgccgatga agcctatatc gagttttgcc cgcaggcatc
     5761 gctggctggc tggctgacgg aatatccgca cctggctatt ttgcgcacac tgtcgaaagc
     5821 ttttgctctg gcgggccttc gttgcggatt tacgctggca aacgaagaag tcatcaacct
     5881 gctgatgaaa gtgatcgccc cctacccgct ctcgacgccg gttgccgaca ttgcggccca
     5941 ggcgttaagc ccgcagggaa tcgtcgctat gcgcgaacga gtgacgcaaa ttattgcaga
     6001 acgcgaatac ctgattgccg cactgaaaga gatcccctgc gtagagcagg ttttcgactc
     6061 cgcaaccaac tacattctgg cgcgctttaa agcctccagc gcagtgttta aatctttgtg
     6121 ggatcagggc attatcttac gtgatcagaa taaacaaccc tctttaagcg gctgcctgcg
     6181 aattaccgtc ggaacccgtg aagaaagcca gcgcgtcatt gacgccttac gtgcggagca
     6241 agtttgatga gtcagaagta tctttttatc gatcgcgatg gaaccctgat tagcgaaccg
     6301 ccgagtgatt ttcaggtgga ccgttttgac aaactcgcct ttgaaccagg cgtgatcccg
     6361 gaactgctga agctgcaaaa agcgggctac aaactggtaa tgatcaccaa tcaggatggt
     6421 ctgggaacac aaagtttccc gcaggcggat ttcgatggcc cgcacaacct gatgatgcag
     6481 atcttcacct cgcaaggcgt acagtttgat gaagtgctga tttgtccgca cctgcccgcc
     6541 gatgagtgcg actgccgtaa gccgaaagta aaactggtgg aacgttatct ggctgagcaa
     6601 gcgatggatc gcgctaacag ttatgtgatt ggcgatcgcg cgaccgacat tcaactggcg
     6661 gaaaacatgg gcattaatgg tttacgctac gaccgcgaaa tcctgaactg gccaatgatt
     6721 ggcgagcagc tcactaaacg agaccgttac gctcacgtag tgcgcaacac caaagagaca
     6781 cagattgacg ttcaggtgtg gctggatcgc gaaggtggca gcaagattaa caccggtgtt
     6841 ggcttctttg atcacatgct ggatcagatc gccacccacg gtggtttccg tatggaaatc
     6901 aacgtcaaag gcgacctcta tatcgacgat caccacaccg tcgaagatac cggcctggcg
     6961 ctgggcgaag cgttaaaaat ggctcttggc gacaaacgcg gtatttgccg ctttggtttt
     7021 gtgctaccga tggacgaatg ccttgcccgc tgcgcgctgg atatctctgg tcgcccgcac
     7081 ctggaataca aagccgagtt tacctaccag cgcgtgggcg atctcagcac cgagatgatc
     7141 gagcacttct tccgttcgct ctcatacacc atgggcgtga cgctacacct gaaaaccaaa
     7201 ggtaaaaacg atcaccaccg tgtagagagc ctgttcaaag cctttggtcg caccctgcgc
     7261 caagccatcc gcgtggaagg cgacaccctg ccctcgtcga aaggagtgct gtaatgaacg
     7321 tggtgatcct tgataccggc tgcgccaatc tgaactcggt gaagtcagcc attgcgcgcc
     7381 acggttatga accaaaagtc agccgtgacc cggacgtggt gttgctggcc gataaactat
     7441 ttttacccgg cgttggcact gcgcaagcag cgatggatca ggtgcgtgag cgcgagctgt
     7501 ttgatctcat caaagcctgt acccaaccgg tactgggcat ctgcttaggg atgcaactgc
     7561 tgggtcggcg cagcgaagag agcaacggcg tcgacttgct gggcatcatt gacgaagacg
     7621 tgccgaaaat gaccgacttt ggtctgccgc tgccgcatat gggctggaat cgcgtttacc
     7681 cgcaggcagg caaccgcctg tttcagggga ttgaagacgg cgcgtacttt tactttgttc
     7741 acagttacgc aatgccggtc aatccgtgga ccattgccca atgtaattac ggcgaaccgt
     7801 tcaccgcggc ggtacaaaaa gataacttct acggcgtgca gttccacccg gagcgttctg
     7861 gtgccgctgg cgctaagttg ctgaaaaact tcctggagat gtgatgatta ttccggcatt
     7921 agatttaatc gacggcaccg tggtgcgtct ccatcagggc gattacggca aacaacgcga
     7981 ttacggtaac gacccgctgc cgcgcttaca ggattacgcc gcgcagggtg ccgaagtgct
     8041 gcatctggtg gatctgaccg gggcaaaaga tccggcaaaa cgtcaaatcc cgctgattaa
     8101 aaccctggtc gcgggcgtta acgttccggt gcaggttggc ggcggtgtgc gtagcgaaga
     8161 agacgtggcg gcgttactgg aagcgggcgt tgcgcgcgtg gtggttggct ccaccgcggt
     8221 gaaatcacca gaaagggtga aaggctggtt tgaacgtttc ggtgccgatg ccttagtgct
     8281 ggcgctggat gtccgtattg acgagcaagg caacaagcag gtggcggtca gcggctggca
     8341 agagaactcg ggcgtttcac tggaacaact ggtggaaacc tatctgcctg tcggcctgaa
     8401 acatgtgctg tgtaccgata tctcgcgcga cggcacgctg gcaggctcta acgtctcttt
     8461 atatgaagaa gtgtgcgcca gatacccgca ggtggcattt cagtcctccg gcggtattgg
     8521 cgacattaac gatgttgctg ccctgcgagg cactggcgtg cgcggcgtaa tagttggtcg
     8581 tgcattactg gaaggtaaat tcaccgtgaa ggaggccatc gcatgctggc aaaacgcata
     8641 atcccatgtc tcgacgttcg tgatggtcag gtggtgaaag gcgtacagtt tcgcaaccat
     8701 gaaattattg gcgatattgt accgctggca aaacgctacg ccgaagaagg tgcagacgaa
     8761 ctggtgttct acgatatcac cgcttccagc gatggccgtg tggtagataa aagctgggta
     8821 tctcgcgtgg cggaagtgat cgacattccg ttctgcgtgg cgggtgggat taagtctctg
     8881 gaagatgccg cgaaaattct ttcctttggc gcggataaaa tttccatcaa ctcccctgcg
     8941 ctggcagacc caaccttaat tacccgcctg gcggatcgct ttggcgtgca atgcattgtg
     9001 gtcggcattg atacctggta cgacggcgaa accggtaaat atcatgtgaa tcaatatacc
     9061 ggcgatgaaa gccgcacccg cgtcactcag tgggaaacac tcgactgggt acaggaagtg
     9121 caaaaacgtg gagccggaga aatcgtcctc aacatgatga atcaggacgg cgtgcgtaac
     9181 ggttacgatc tcgaacaact gaaaaaagtg cgtgaagttt gccacgtccc gctaattgcc
     9241 tccggtggcg cgggcaccat ggaacacttc ctcgaagcct tccgcgatgc cgacgttgac
     9301 ggcgcgctgg ctgcttccgt attccacaaa caaataatca atattggtga attaaaagcg
     9361 tacctggcaa cacagggcgt ggagatcagg atatgttaac agaacaacaa cgccgcgaac
     9421 tggactggga aaaaaccgac ggactaatgc cggtaattgt gcaacacgcg gtatccggcg
     9481 aagtgttaat gctgggctat atgaacccgg aagccttaga caaaaccatc gaaagcggca
     9541 aagtcacctt cttctcgcgc actaaacagc gactgtggac caaaggcgaa acgtcgggca
     9601 atttcctcaa cgtagtaaat attgcgccgg actgcgacaa cgacacgcta ctggtactgg
     9661 cgaatcctat cggaccaacc tgccacaaag gcaccagcag ctgcttcggc gacaccgctc
     9721 accaatggct gttcctgtat caactggaac aactgctcgc cgaacgcaaa tctgccgacc
     9781 cggaaacctc ctataccgcc aaactgtatg ccagcggcac caaacgcatt gcgcagaaag
     9841 tgggcgaaga aggcgtagaa accgcactgg cagcgacggt acatgaccgc tttgagctga
     9901 ccaacgaggc atcggacttg atgtatcacc tgctggtgct gttgcaggat caggatctgg
     9961 atttaacgac ggtaattgag aacctgcgta aacggcatca gtgagttgca gggtaagcgg
    10021 atgcgatgtt gttgtcgcat ccggcaaaaa aaaagggcaa ggtgtcacca ccctgcccgt
    10081 tttctttaaa gctgcaaaga ttacttcgcg ttataattac gcagagcgtt acgccccagc
    10141 acaatccccg cgccaaccat gccgcccagc agcaccgcca gaatcaaggt aattgccttt
    10201 ttcgggctat cgcgacgaat aggtaacgtc ggtttcatca cataacggta agcatgaata
    10261 tcaagatcat caacctttaa gttttcaata tccaacaggt tttgacgtgt ctgatagtag
    10321 tttggtgaga acaccaacgg acgggtcgcc tcatgcttaa tcatcgactc cagcgcttcg
    10381 ctccccaaaa ggaacaacgt atcttgtgtc acatcttcag tctgctgaac ctgtggcttt
    10441 gccacctgcg cctgattcgc atactgcaac gcttcctgaa tctgacggat acgcagatct
    10501 ttctgctcct gcgcgactac ttcctgcgtt cttaaagagt cctgcaagtt tttccgtccc
    10561 agagcaatgt tgtctttgag atccttttct agctcttgat tcactttatc atcaacttgc
    10621 tgaatgtatt gggccaactt catttgtgct ccctctgcag tttgcccaac ataagaaaca
    10681 gtcaatggta attgctggtt cttaacagaa ggttcgatgg taagtttttc tggttcttcc
    10741 tgattatcca gcgtttctgc taatgcagag aaggcagaac tgaagcgacc aattaacgtc
    10801 tcctgtaaat ccgatacttt cggtgcagcc tgaccataga taacattcat ggcattgtta
    10861 tagccagcaa tttgccccac gtcgggctga gtgataattg ctgttgacgt ccatttctcc
    10921 ttcgctactg ccaaatatcc aatagccagg gcaatagcca caatgacgga aatgatgatt
    10981 gtcatcttgc cacgccacaa ctgcgctagt aaatcaatca aatcaatctg ttccgggtca
    11041 tggttttgcc cagaaacatt attattttct actctcatca ttaccctaac tgacggaaat
    11101 ttccaagtcc gcatagttta gcgataatcc tagtgaatgc tataggataa gtgataaaaa
    11161 taatgaatta ttggggaggt aacaaaagac aatcagggcg taaatagccc tgataacaag
    11221 atgttagtcg ctgccaaaga gatcgcgggt gtatacctta tctgccacat ctttaagctc
    11281 ttctgccatt cggttagaga taatgacgtc ggcttgttgt ttgaagatgg cgagatcacg
    11341 ttccagccga gagttgaaga atgagtcttc tttcatcact ggctcgtaga tgatcacttc
    11401 aacgcctttc gccttgatac gcttcataat cccctgaata gaagacgcac ggaagttatc
    11461 tgaaccgctc ttcataatca gacgataaat acccaccact tgcggtttgc gtgacaaaat
    11521 ggcatcggca ataaaatcct tacgcgtgcg gtttgcatcg acaattgccg agatcaggtt
    11581 attcggcaca gactgatagt tcgccagtaa ctgcttggta tctttcggca agcaataacc
    11641 accataacca aacgacgggt tgttgtaatg gttgccaata cgcggatcga gacaaacgcc
    11701 ttcgataatc tggcgagtat tcagacctaa actttctgcg tagctatcca gttcgttaaa
    11761 gtatgccacg cgcatcgcta ggtaggtgtt tgcaaaaagt ttaatcgctt ctgcttcggt
    11821 cgaatcggtg aacagggtcg ggatattttg cttaatcgcg ccttcctgta acaacgcagc
    11881 aaaacgttct gcgcgttctg aacgctcacc gatgacaata cgtgaaggat ggagattatc
    11941 gtaaagggct ttaccttcac ggagaaattc cggagagaaa ataatatttt cagtacgata
    12001 tttcttatgc atcgcttcgg taaaaccaac gggaaccgtt gatttgatga ccataaccgc
    12061 ataaggattt atctcaacta cgtctttaat tactgattct acactggatg tattgaaata
    12121 attagtttta ggatcatagt cggttggagt ggcgatgatg acataatcag catcccggta
    12181 agcttcattt ttatctaatg tggcattaaa gtgtatttta tctgattgca aaaactgctg
    12241 aatttcctta tcaacaatag gagatatccg atcattcagc atagcaacgc gtgacggtaa
    12301 aatatctaat gccacaacct catgattttg tgcgattaga agcccgtttg acaagcctac
    12361 atagccagta ccggaaatgg tgattttcat ttcatgctct cagaattaac ttaactgtga
    12421 atcatgatgt ttttagcatc ctgataagag ctaaatgttt taacgctacg ttattagctg
    12481 gtcaacgcta cgaaaaacaa ttcagatttt ctttataaga atagctgata tttattaagt
    12541 taatattaag cagtgaaatt tagtctataa gataaatggc aaaaaaaaag cccggtgcaa
    12601 tatacgccgg gcctcaattt tactgttggt taaatcagat taatccagcc attcagtatg
    12661 gaacacacct tctttatcaa tgcgcttata agtatgtgca ccgaaatagt cacgctgtgc
    12721 ctggatcagg ttcgcaggca gaacagcggc acggtagcta tcgtaatagg caaccgcagc
    12781 ggcgaaggtc ggaaccggga taccgttctg tactgcataa gcaacgacat cacgcagcgc
    12841 ctgctggtag tcatcggcaa tttgcttgaa gtacggagcc agcagcaggt tagcgatctg
    12901 cggattttcg gcatatgcat cggtgatttt ctgcaggaac tgcgcacgga tgatgcagcc
    12961 agcacggaaa atcttcgcga tttcgccgta gttcagatcc cagttgtatt cttcagacgc
    13021 agcacgcagc tgagagaagc cctgagcgta agaaacgatt ttgcccagat acagcgcacg
    13081 gcgaactttt tcgatgaact cagccttgtc gcctgctgac tgagcttgcg gaccagagag
    13141 aactttagat gcggcaacgc gctgatcttt cagagaagag atataacgtg caaacacaga
    13201 ctcggtaatc agcgacagcg gttctccgag atccagcgcg ctctggctgg tccatttacc
    13261 ggtacctttg ttagccgctt catccaggat cacatcaacc aggtagttac cgtcttcatc
    13321 ttttttagtg aagatatctt tggtgatgtc gatcaggtag ctgctcagtt caccgttatt
    13381 ccactcggta aaggtctgtg ccagttcttc gttagagaga ttcaggccgc ctttcaggag
    13441 agaataggct tcagcaatca gttgcatatc accgtattca ataccgttgt gaaccatctt
    13501 cacataatgg cctgcgccat cagcaccgat ataggtaacg cacggttcgc catcttcagc
    13561 aacggcggcg attttggtca ggatcggcgc aaccagttca taggcttctt tctggccacc
    13621 aggcatgatg gacggccctt tcagcgcccc ctcttctccg ccggaaacac cggtaccgat
    13681 gaagttaaag ccttcggcag aaagctcacg gttacgacga atggtgtcct ggaagaaggt
    13741 gttaccacca tcgatgatga tgtcgccttt atcgagatat ggtttgagcg aatcaatagc
    13801 agcatccgtg cctgcacctg ctttcaccat taacaggatg cgacgaggcg tttccagaga
    13861 ttcaacaaac tctttcaccg tatagtaagg aaccagtttc ttgcctggat tttcggcaat
    13921 cacttcttcc gtcttttcac gggaacggtt gaaaatagag acggtataac cacggctttc
    13981 gatgttgagc gcaaggttgc gccccatcac tgccataccg actacgccga tctgttgctt
    14041 tgacattgtt tactcctgtc agggggtggt caccgccgag tgtgcggctt aaatgtggta
    14101 tagatgttaa ctcactcata gagatggtga atagcattac ctttgctata ccacactgaa
    14161 tatctcacct tttccgtctt ttaaaactta tttatatgaa attactgaat tacttgttca
    14221 gtaactcaag aataagtttt gtcttttctt ccatcagcgg cacatcaccg cgagattcca
    14281 cattcaaccg caccaccggt tcggtgttgg aggtgcgcag gttaaagcgc cagtcggcaa
    14341 aggtcatgct gatgccatcg gtgcgatcca ccgccagcgc ctcgcggcta aaatgctgtt
    14401 ccacgcggtt aatcgcctca aagggttgcg ccagtttgct gttgatctca ccgcttgccg
    14461 gaaacgctgc catccggtcg cgcaccagtt cgcccagcgt ttttcctttc aggcacacca
    14521 gttcggcgac cagcagccac gggatcatgc cgctgtcgca gtaagcgaaa tcacggaagt
    14581 aatggtgggc gctcatttca ccaccgtaga tggcgtcttc cttgcgcatc cgttctttaa
    14641 taaaggcgtg tccggttttc gacatcaccg gcgtgccgcc tgcggcagtc accacatcaa
    14701 cggtgttcca ggagagacgc ggatcgtgga tgatcttcgc gccgggattt ttttcgagga
    14761 atgcttctgc cagcaggccg acaatgtagt agccctcaat aaactgccct ttttcgtcaa
    14821 acaggaagca gcggtcaaaa tcgccatcaa aagcaatgcc catatccgca ccgtgtttga
    14881 tgaccgcatt gcgggtgtcg tcgcggcatt ccggcagtag cgggttagga ataccgttgg
    14941 ggaaattgcc gtccggcgtg ttgtgcactt tgattaattc cacgggtgcg ccaagggctt
    15001 taaagcgggc ttcgatggcg tccaccaccg gacccgctgc gccattcccg gagttaatca
    15061 ccagcttgag cggcgtgagg tttttgacat tgatataacc gaacaggtga tcaacgtaag
    15121 cgtcacgcag attgatttgc tgatagcgac cgcgtttggt ttcatcaacg ggaggaaagt
    15181 cgttggcttc tgccagacgc tggacatcgc gcagtccggt atcaccgctg atcgggcgag
    15241 ccccttcgcg caccagtttc atgccgttgt aatccatcgg gttatggctg gcggtaactt
    15301 caatgccgcc atccacgccg agatggaacg tggcgaaata gatctcttcg gtgccggaca
    15361 tgccaatatc cagcacatcg acgcccgcat cctgtaaacc tctcgccagc gccagtttta
    15421 aggtttcgct ggtgaggcgg acatcaccgc ctaacacaat ggttttcggt ttgagaaatt
    15481 cgccataggc gcgaccaatg cgccaggcga tatcttcatt cagttcttcg cctaatttcc
    15541 cgcgaatatc ataggcttta aagcaggtta attttttcat gtcgttaccc tttttcaggc
    15601 aatagttggc cctgaccgaa acggccaatt ttgtttttgt cgttatttac atctcggttt
    15661 cgcattcatt gcctgatgcg acgtttacac ccgtccgtag cgatccgcga aacgcaccac
    15721 atcatcctct tcgagataag agccggagcg cacttcaatt aaatcgagcg gaattttccc
    15781 cgggttttcc aggcaatgca tcgcccccag cggaatataa atggactcgt tttcaccaag
    15841 cagtttgata tcaccatcaa tggtgacttt tgccgttccc gcgacaacca cccagtgttc
    15901 cgcgcggtga tggtgcatct gtaccgacaa gccctcgccc ggtttcacgg tgatgcgttt
    15961 cacctggtag cggtcgcccg cgtcgataga gtcatatttg ccccacgggc gatagacttc
    16021 gcgatgcacc cgatgctcat ggcgaccatc ggctttgatc tgctcgacca cttttttcac
    16081 atcctgcacc gcgttacggt cggcaatcag caccgcatct ttggtctgca ccactaccag
    16141 atctttcacc ccgacggtgg ttacgaggcc agattcggcg tacacatagc tgttttcagt
    16201 tttgtgatta atcacatcgc cgtggcaaac gttgccctcg gcggtgtggg cgctaatttc
    16261 ccataatgag gaccaggagc cgacatcgct ccagcccgca tccatcggca ccacaacggc
    16321 atctgccgta cgttccatga ccgcgtaatc caccgactct tccgggcagg cgagaaacgc
    16381 ttcttcatcc acacgaataa aatcgagatc cggatcgacg gcgctcatcg ctttttcaca
    16441 ggcatcgaga atatccggac gatatttttt cagttcttcg agatagcgtc cggcgcggaa
    16501 caggaacatg ccgctgttcc agtaatattc gccgcttgcc acataggcct gggcggtttc
    16561 cagattcggt ttttcgacaa actgcgccac ttcaaaggcc accgtatcct gctcacccgc
    16621 agacacttca ccgcgacgaa tatagccata accggtttct ggtagatccg gcacaatgcc
    16681 gaaggtcacc agcttgcccg cttcggcata tggcatggca ttacgcacgg cggcacggaa
    16741 cgcgtcttca tcggcaatca catgatccgc cgccaatacc agcattaacg ggtcgctctc
    16801 cgggctatga cgttttgccg ccagcgccgc cagcgcaatg gcaggtgccg tgtttcgccc
    16861 tgccggttcg agaataatgt tctcggtaag tttgttcagt tgacgcagct gttccgcgac
    16921 aataaagcgg tgctgctcat tgcaaatcac caccgggctt tcgcactcca cgccgttcag
    16981 gcggcagatg gtggtttgca gcatggtgag atcgcctttc aggcataaaa actgcttggg
    17041 gtaaagtacg cgggaaagcg gccataagcg gctaccggag ccacctgcca tcacaactgg
    17101 atagagtttc gactgcgcca taattatccc cgaatatcat ttataaattg acgtaacacg
    17161 ttctctttat cgagtgtgcg ttcggcatat tcacgtgcca ccgtgttgtg tttgggcagc
    17221 aggagcgcct gacggatccc cgccaccagc gcctcgaccg attccggttc tacgcaaacc
    17281 gcaatgcccg gaaaggtttc gcaaagctgc cccagttctg tgtgggcctc tgcggtaatc
    17341 accgcgttac cgcctaccgc cagaatattg gtcagtttcg acggcaatac ggcatctgcc
    17401 gcgccgcgtt tttgcaccac cagatggcaa tcgcccatct tcagcagtgc gggtaaagcg
    17461 tcatacgatt gcagcgggaa aaattgcatg ttgcgcagtc cacgctgctg cgccattttt
    17521 tccagccgcg ctttgccgcc gccctgcccg acaatggcaa aaatcagcgg ttcatcgcgc
    17581 agacgatcgg cagcttcaat aacgttttcc agcccctgct tttcaccaat attgccggag
    17641 taaagaatga tttttttgtt atccggcagg tcaagctggt tacgaagggc gtcaacatcg
    17701 gcatctgcaa catgctgaaa acgggcaatt tccgaccagt tggggaagaa gatgacgttt
    17761 tccgccgcca cgcctttttc gatggcttta ttcatcatcg aacgcgaaat cgtggagacg
    17821 ttatcgacgt tatgcagtcc gctacgttcg aacgccgttg ccagctgtgc cactttgctg
    17881 cctttgcctt ttccggcaag gcccagcccc agcatggcgt ccacttcgta atcctgaata
    17941 tgcagcacgg tacgcgcacc agaaagtttc gccagcaggc gcattcccgg cgtgcaaaac
    18001 agcgttggca ccacgccgat aatgcgatcc ggcttccagc gacgttgcgc catcagagga
    18061 aagaaactgc tgacggcaaa actgccgaga tgtaacaggc gtttcagggt gctcggctgt
    18121 tttggcacgt acagcgggca gcgccacacc gtggcggccc cctcttctcg tttgtaccgc
    18181 caggcggaat agttctcgcc cacctgccac tgcgggtagt aaggcggtgc ggtaataacc
    18241 cgcacctcat gaccttgtgc cgccagccat tccaccatct cgccggtgta tttgccgata
    18301 ccggttaact ccggcgagta gttaatgccg tagaccagta ttttcataat ccgggtactc
    18361 cggcacgctt ctcagcgaga aaataggcgc ggctgttagc gtgaacatcg ttactggcga
    18421 gcagcgcgtc cggcgtcagc cagcggtaat cgtcatgctg ctcatccggc agtatcagct
    18481 cttcttcagc gactctgaag cgaaaaccga gcaccacata gtgagtggtg aaatccgtgc
    18541 cggagaagtt atcgtcataa aagtgctgcc agacaccgta aaactggcct gctgttatcg
    18601 gcagacgcag ccccagttcc gccatcgtca gccgctcgaa tgcggcttcc agcgtttcgt
    18661 ctttctgcac gcgccctccc ggcacaaacc agtaaccctg cgccgggcgg ttggttcttt
    18721 tgccaagcag aaactcaccg cgactgttct cgacaataaa gtcgagagag acaagcgggg
    18781 tggagcgcac taccgtggca aagtcttcct gacgtaaaaa catcattacc cccgaaagcg
    18841 gtcttgattc tcaaggaacc actggtaagt gctggcaagc cccgcttcca gtgagatttc
    18901 gtgataccag ccaagctgat gcaggcgcgt cacatccagc agtttgcgcg gcgtaccatc
    18961 cggtttgctg gcatcaaaaa ccacccgacc tttgtaaccc accactttgg cgatggtttg
    19021 cgccagctcg cggatagtgc agtcaacgcc cgtgccgacg ttaatgtgcg acagcatcgg
    19081 ctgggtgttc tccagccaga cttcgtgcgc cagctccatg acatgaatgc tcgccgccgc
    19141 catatcatcg acgtgcagga attcacgcat cggtgtaccg ctgccccata ccaccacgtc
    19201 cggtgcattc tgtgccgtcg cctcgtggaa gcgacgcagc aatgcaggga tcacatgcga
    19261 attactcggg tggaagttgt catgcgggcc atacaggttg gtcggcatga ctgagcggta
    19321 atcgcgtccg tactggcggt tgtatgattc gcacagtttg atcccggcga ttttggcaat
    19381 ggcgtaaggc tcgttggtcg gctccagcgt gccctgcaat aactcgcttt ctgccatcgg
    19441 ctgttttgcc agtttcgggt agatacagga cgatccaaga aacagcagtt tgttcacgtc
    19501 gttctgatgc gcggcgtgaa tgatgttgct ctcaatcatc atgttctggt agatgaaatc
    19561 cgccggatag gtgttgttgg caacaatgcc gcccactttc gccgccgcca gatagacctg
    19621 gtcaatgcgt tcgctggcaa agaaatcatg caccgcgcgg ctgtccagca ggttcagctc
    19681 gtcgcgggtg cgtaatacca gttccacatc accgcgctgt tcgagctgcc gcctgatggc
    19741 agaaccgacc atcccacgat ggccagcgat aaaaatgcgt tgtttactca tgcttatgac
    19801 tccagcgcga tcgccacgtc gtagccgtga gatttcagca gagagtgttt tttcgccgct
    19861 tcgaggtcat tagccaccat ttcagacacc atctctctga gggtgatttc cggtttccag
    19921 cccagttttt cgtgcgcttt ggtcgggtcg ccgagcagcg tttcaacttc agccggacgg
    19981 aagtaacgcg gatcaacggc gataatcaca tcacccggtt taacgcccgg cgcgtcatgc
    20041 ccggtgacgg aaaccacaat gcccttctct tcaacacccg tgccttcaaa gcgcagtttg
    20101 atgcccagct gtgccgctgc catttccacg aactgacgca cggagtactg aacgccggtc
    20161 gcgataacga aatcttccgg ctgttcctgt tgcagcatca tccactgcat ttttacgtag
    20221 tctttggcgt ggccccagtc acgcagggaa tccatattgc cgaggtacag gcacgactcc
    20281 agcccctggg cgatgttggc gattgcgcgg gtgattttgc gggtaacgaa ggtttcgccg
    20341 cggcgcgggg attcatggtt gaagagaatt ccgttacaag cgtacatgcc gtaggattcg
    20401 cggtagttaa cggtgatcca gtaggcgtac agtttggcga ccgcatacgg agatcgcggg
    20461 tagaacggcg tggtttcttt ctgcggaatt tcctgcacca gaccatacag ttcagaggtg
    20521 gaagcctgat agaaacgagt tttcttttcc agaccgagga agcggatcgc ctccagcagg
    20581 cgcagcgtac ccatcgcgtc gacgtcagcg gtatattctg gtgactcaaa agagaccgca
    20641 acgtggctca ttgcgcccag gttgtacact tcatccggct gtacttcacg caaaatgcgc
    20701 gtcaggttag aggtatcact caggtcgcca taatgcagat ggaatttcgg gttgcaggtg
    20761 tgcggatcct gataaatgtg atccacgcgc tcggtgttga atgacgatgc gcgacgctta
    20821 ataccatgca cctcgtaacc tttttccagc agaaactctg ccaggtaaga accgtcttgt
    20881 ccggttacac cggtgatgag agcgactttt gacatgtctt attcctctgt atttttgaat
    20941 ttattcagtt tcaacgcgtt cgcgtatcac cactgcggga ttcccccggc aaaccacatt
    21001 tgccggaagc gatttaaaaa cactgcttcg tgcacccacg acggtgccgt cgccgattgt
    21061 gacgcctggg gcaacaaaga catcggttgc cagccagcat ttctcgccaa tcacaatagg
    21121 cgtggcgtta atggtgaaat gttgacttgc atggtcgtgg ctaccggtgc ataaataact
    21181 tttttgcgat atcaccgaat gtgcgccaat ggttatttca ccgagggtat ataaattgac
    21241 gtcatcgccg acccacgcgt aatcacctaa ggttaatttc cacggatagg taatttttac
    21301 tgacggacga ataactacgt tttttcctat ttttgctccg aataaacgta ataaaaaagc
    21361 ccgccagcga tacaatactt gtggcgacca ggcaaatatt gttgcctgta ctgcccacca
    21421 taattgcact ttaatagcgt tgccgccccg gaaccctttc ggcaccgaga aaccgcttaa
    21481 atcttgcatt ggttttcctt atattcagac tttgttatat aaggctttcg tctttgaggt
    21541 agtacgttgg cgtaaatgcc aggataattc agcccaaaag ccaggcacat gtaatatttg
    21601 tcgttggact tttttcgcgt cagcacacaa ttccatatta ttggtggtag ataccccacc
    21661 catggaaaat tcagacacca ggccattgag ttttttaaat gcataaccgg ctttatacat
    21721 tttggctgcc agcgcgtagt cggaagaaac tttatattcc aggtcataac gccatttttt
    21781 caagccggat acagggaaaa atatcgcctg atgactggcg ggcaggctgt gataaatata
    21841 ccagcccggt ttggcgctac gtttaatttt atgcccgtcg ccaaaatcca gcagcgcatc
    21901 gccggtgatc atcatgttat ctttttgcat ttttaactta cggacaaaat ttgcggcatc
    21961 ctgatgaaaa atatcgcccg aattgagaaa caacgcgaac ttgccttgtg ccatcgtaat
    22021 gcctttgttc atggcgtcgt agataccgtt atctggctcg ctgacaaagc gtaggttata
    22081 gataccattg agattttcca gatactcacg ggtgccgtca ttggaaccgc catcgacaac
    22141 aatccattcg aagctgatat cttccgcctg cgccagatgc gccagcgagg catgtgtttt
    22201 gactatccct tcgaggttac gaaacgcgac agtgattatg ctaagcaaca tgttcttatt
    22261 cttacctcgt aatatttaac gcttttcgca aaataaacgg acagacgatt aaaaatgcat
    22321 attccgggct aaatatcgaa ccggtaaaaa acagcgatac cggagtaaaa agatataatt
    22381 gcacgcgaaa attgcggtta tcaccaaaag cgttgatcat catttttatc actttcccca
    22441 tgtaccacag cgataaaaac actgcgaacc aggaaaaata aataatcagc agatacaaac
    22501 cattgtctat ggtttttccg acatccgcac cgttaaatat tccgaatgat gcgacgtatt
    22561 cataaagtga gccaaatctg actacaccgt caatatgggt caaagaataa ccgaccatca
    22621 ccagcggacc gacaatacga taatacgatg acgatccttc cgtacctaaa tcgcccagac
    22681 gggtggaaat ataaggaaac gcgattacca caccaacccg gaatacagcc agagaaatca
    22741 atgccaacgg taactttttc ttaatcgcct ctttattcag atattgaaat gcccactcca
    22801 gcaaataaaa caggataaaa gtcataaccc ctgaaaacga tccggataat attatccctg
    22861 cgagaatcat agcatcggtt ttaggcgttt tgataccaaa ctgtttgatg ctgagccaaa
    22921 ttgagattaa tgccagagcg aaaaatgccg gttcgaaata aagtgctgtc gtgcgcttgc
    22981 cgccgaattt aatgaaattc agtacatagc tgttgctgta aatcagatat ttcgaaatcg
    23041 actccataat actgctgcca ccggtgagga taatttgcgc catctccacc gccgccagcg
    23101 ccaccaccag cccgatcacc agataaaaga aacgtaatat cttgcgatgg ttgtgtggcg
    23161 aaatcgtttt aaagcgaata ctccacacca tgccaataat gatcacaata tagacaaaca
    23221 gcatggttga agtgacgtat ttactggcat ccagcgactg accaaacaga tagttaaacg
    23281 ccgtgagtcc cgcaccaatc cctaaggcaa tcatcaattt tttaacgctg atgcgttcta
    23341 aaaacagcag taacaagaca ggtaaaaaag tgacgatggt gatggggaaa ctttcgccaa
    23401 gctgggcgat tttgacgtta accagcaggt agatcagcgg cagcagcagg tagctacaga
    23461 ttctgataga agttgacata ctcctccagc atctgttgtc cactgtaggt ggcgcggctg
    23521 cgttggctga actcagccag cgtggtacca aatatcgcct gcgcgatttc cggtttgctt
    23581 aactgcacca gttgcagcac gtcttcttcg tcgacggttt taccgccgga tttttgcaac
    23641 acttcccgtg ccgcgtcgct atgggtggca atcaccggca cgccaattga taacgcctca
    23701 cacaaaatca gcgggtagtt atcgacgcga gaactgaata ccagcgcatc catctgattg
    23761 agcgcgctca tcaacttgcg cttgtcagtt tcaaagccgt gattaaccac gttgccagcg
    23821 gtgaacggcg agaacttacc aaaggtatgc agttcgattt tgtcgcccag cgccatcatc
    23881 tcacgcacca gttgctggtt agtcttgccg tcgtaacgca agtcatgcgc caccaccgcg
    23941 attttcggct tgccctgggt ttcgcgcacc ggaggcaagt ccgccagaat cgcttcggtt
    24001 gccatatcaa taccattatt gataatccgg caacgccctg gaccgtacag gctattgaaa
    24061 gcgtcagcca catgctggct gggggaaata aactgacagc ccagcgccag catctcacgg
    24121 aataactggc gtttgcccgc caccagctgg tgtgcgcgat caatcttcac cggcggatag
    24181 ttatttaagg tcgggcattt ctggcagccc gttttccagc cttcgcaacc gtcggtaaag
    24241 gcgcagcgtc cggtaacact ccagtggtcg tgcagcgtcc agaccagagt gacgtccggt
    24301 ttatggtttt tcactttttc gcaaaagcgc accacgctct taagatttag ccagtagctg
    24361 tgcagcacat gaaaatgcag gaccaccgga cccggtgtgc gagtaatggt gcgatataac
    24421 tcattgaaat tgccaaacag atcgcgatta aacagacgaa acagggcaat attcgccatc
    24481 gcggtcatcc gcggcgtatg tttgatgacc tgcggatagt tctgatggct gacgctctct
    24541 ttgccgcctt tgccataacc gtagacaaaa tgtgacgcca gcccctgttg cagcgcacgc
    24601 tggtggagat ctaacgccac acctgctgcc ccgccttccg ccagtcgcac attaaattgc
    24661 agaatattca tttaattacc ttcactcgcg ctttttctcc caccaccagc gcgttgtccg
    24721 ggacagagtc gagcaccacg ctgcccgcac ccacggtgac gttgttacca agcgtgatat
    24781 caccaagaat aatgacgttg gcaccgagtt cgacgccgtt gccaatgtgt ggacatgcca
    24841 tgttatcggc accacgattg ccgatagtga cgccgtggcg aatggtgaaa tcatcccccg
    24901 ctaccacgtt tttattgatc actacggcgt aaccgtgatg gatagtaaag cggcggccaa
    24961 tggtcgcggc ggcctggatt tcataaccga aaaagcattc ggtgataatg cgatacagca
    25021 ccagcagcgg ggccgcccac agattgttga ggacgttctt tttgcgccac accgaacaaa
    25081 aatgagcaac acgataggca agaaccatgc agcacgggcg taaactccag ctgttggcac
    25141 gcagatcttc cagcatcttt agcgcccccg aataccatca gccagacgtt tgccgttacg
    25201 caccgacagc agcgtcagca aagtgcgcca ggtcatacgt ttattgcgga tctggtagag
    25261 ggtaaacagc tgatattttt tgcttgcacg gtcaaattta tctttgtgct ttcggtaaaa
    25321 gtggaagtac ccggagaatt ttttcggtga cgaggtgatc tgcatttcac cgtgattgat
    25381 atgcaggatc tgcgtcgctt cttccacttt ccacggttcg ccgtactcca ccaccatccg
    25441 caggaagatg tcgtaatcct gtgctgcttt cagttcggta tcgaacaaac actctttgaa
    25501 acgccatgcc caagtaaaga cctgattgcc aatgatattg cgtttataga acaggcggcg
    25561 tgagtacggc gatttgggat acagtggtaa gcttgccggt tgggaataaa cttctccctg
    25621 gcatacgtag tcgttagcgt acaaaaaggc gtgagtgacc agttgctgtt tatgggcgag
    25681 gaagacgctc agacggttgg gtgtccattc gtcatcgtca tcgatcccgg taatgtattc
    25741 cccttgcgcc agcataatcg cctggttacg taccgcgcac gccccgctgt taatgtcgtt
    25801 gtgaatgtag gtgatacgcg gatcgttgag ggcagtgacg tactgttgca gttgttccca
    25861 ggaggtggag caatcatcca cgatgatcat ctcccagttg ctgtagtcct ggcgcaggac
    25921 cgattttatc gcccgaatcg ccagttgttg acggttccag gtcggcatat agattgaaat
    25981 cagcggattc tctttcatag gttgttctcc cggcttatga gctggtttgc ttgccggatg
    26041 cggcgtgagc gccttatccg gcctacgggg cggtgcgaat gcaggcgtcg ttatttcgca
    26101 tccgacttat attcgtattc gtaatagcca taatcctgat acgcgctggc gcggcggaag
    26161 atggagttca gaatcacccc tttcaccgga ataccgtttt gctcaaagcg gctcagactg
    26221 gtttccactt ctttcaatgt gttgaccgca tagcgcgcca ccattaacgt ggttccagcg
    26281 tgacgcccga caattgctgc atcggtcact gccagaatcg gcggcgtatc aatcaacacc
    26341 aggtcgtagt ttttactcgc ccagctcacc aattcggcaa agcgttcgct catcaacagt
    26401 tcagaaggat ttggcggcac ctgaccgcgc gggatcaggt caaatttggc aatagaggtc
    26461 ggtttagctg cggtagtaat atcgccctgg ccaatcagaa tttccgacag gccattgacg
    26521 ttattggtac ccaacagctc gtgggtgtag cctttgcgca tatcgcagtc gatcaacaac
    26581 acccgtttat tggtctgact gatcaccgcc gccaggttgg cgcagacaaa ggttttaccg
    26641 attgacgggc tcacccctgt catcatcaac acattgttct gcgcctgcat catcgcgaag
    26701 tgcagactgg tacgcagact gcggatggct tcaatcgcca gatcggttgg attccccacc
    26761 gccagtagct ggctctgttt atagcgttta atccctttga tggttttgac gctatcgcgc
    26821 gctttctgcc attccgacag cgggatgctg gcatagacgc tgataccatg ttcttccagc
    26881 acctgcgggc tttcgatgcc gcgattaaac aacgagcgca gcagcacacc cacgatagag
    26941 agcatcaggc cgaggataat tgcgccgagg ataatcagtc ctttcttcgg tttcagcacg
    27001 ccaggctggg tgattgccgg gtcaacaatg cgcacatcgc cgacggtgct ggcttcggtg
    27061 attttcagct cctgctcttt attcagcagt tgcatataga cctgctgacc agactcgaca
    27121 tcgcgggtca ggcgcacaat ctcctgctgg gttttcggca tcgccgttac gcgaccgtta
    27181 agtttggctt tctcttcttc cagcgcctga cgtttctcca gcagcgtgcg gtacgccggg
    27241 tgaactttgg tgtacagctt ggagatttcc gcctctttaa aggtcagttc gttcaactgc
    27301 gcgtcgatgt tcaccatcga atcgagcacc gctttcgctt ccagcggcag atcaacagaa
    27361 tctttatcct gacggaaggc attcagtttg ttttcggcaa catcgaggcg gctacgtact
    27421 tccggtaact gttgcgcgag gaaagcgagg cttttcgacg cttccgccga tttgcgctca
    27481 atattttgtt cctgatagtt acgggcgatg ctgttaagaa tgtcgcggat ctgttcgcga
    27541 tcttcaccgg tataagtcag gctcagtacg cctgcgtctt tgccgttctc cgttaccgtc
    27601 aggctgtttt gcagttggtt gatcatcccc aacgtggagt atttggtgac ggtaaactca
    27661 ctgcccgggc gggcgtgaat ggcttcaacc atcagcgtga cgccttcttt tttcagcatc
    27721 tggcccgctt gcccacgggc gctaaagccg ccatcactgc tcagggtgta gtttttattg
    27781 tccagcacat taagcgtaaa cacctggccc gccatctctt tcgggcggtt aaaggtagtc
    27841 actttcaccg tctcgttctg gcgtcccatc agtcgctccc agcccgcccc gaaaatcggg
    27901 aacgtgtttt tgctcactgc aatatcgagg tcgagatcgt ccaccgtttt accaagcacc
    27961 agacgcgaac gaatcaactg gatctcggcg tccgatgcag gcggtttgtt ggctaatgcc
    28021 gaaccaatgt cctgcactaa cgaattgccg ctgttttgct cgatttgtac cagtgcgtcg
    28081 gcactataaa tcggcgttgc gaagaaggtg taaaccacgg cacagagggc aaatacagcg
    28141 gtgatgccaa tcacccacca gcgcgcttca atgacggtgc cgaccaggcg accaatatcg
    28201 atttcatcac tgcccgttac cggagcggca tgttgtttta ctttttctgt cattcttata
    28261 cctgctctgc gttcaatgcc tgcgcccact ggcgggcaga ccgttcaagt aatgtgtaca
    28321 ccgctgcaaa cgtttcccgg cttttgcgat acggatcggg gatttcacat tcgttatccc
    28381 agtgaccaaa cagcatcact ttgccgcgca tctcaggtgc catctcgcat aagcgttcga
    28441 tatggcgctt ttccatggtc aaaatcaggt catagttgcg acacaggcgg cggctgattt
    28501 gtcgtgcaca gtggccttcc agagacagtt gatgttctgc ggcgacgctg atagcggtag
    28561 gatcagcgcc cttaccgacc agcgcgccga gtccagcgga ctccactttc agttccgggt
    28621 gataacgttg cagtaagcgt tccgccgtcg gggaacggca aatattgccg acacagacaa
    28681 ctaatatgtt gttaaacatg acgattacca gttatgaatg tcgctggctg tatccgtcat
    28741 gtaacggaca ccgctaatag ttggcaacaa ctgattgatc agacggttcc agcgggaaac
    28801 cggggcggtg gtgacataca ccacgtcata aggttgcagg cggaattctg tcgccatcac
    28861 cagcgacgtg gcatcggaca tatccagctg gtagatattg gcaatcttgc cattacgccc
    28921 gccctcgcct ttcagcggac gaatgacaaa gatgccgctg gcgttggagg tggtcatgtc
    28981 gataccttca gcattaccca gggcttcagt cagggtcatg ccgctaaagt ccattttcag
    29041 ggtgctctgt ttcttcactt cacccatcac aaatactttc agatcatcat tacgcggcac
    29101 gtagagaata tcgccgggat aaagcagtcg gttctgattg aggtcgccgt tttgcatcag
    29161 cgcctgcaaa gaaatgcgtt cttcacgacc attgtgtgtt agcactacgt tgcgccagtc
    29221 agcggtgtcg gtcaggccac ctgcggcgtt gatggcgtcg agaatagtca gtggcacgtt
    29281 ggtgatcgcc tgttgaccgg atttattcac ctgaccggag atataggcct tttgtgagcg
    29341 gaaggcggcg atattaacgt ccacctgcgg gtcagcgatg tacgtcgcta agcgcccggt
    29401 aatatcactg cggatttcag cgagcgtttt cccgactacg tgaaccttgc cgatatacgg
    29461 gtaaaacata gtgccgtcag gctgtaccca gttgccggtg tcgctggagc tgcggtactg
    29521 accggctggc gtggtgagtt ccgggtgatc ccagacggtg acattcagaa cgtcgcccgg
    29581 cccgacgcga tactggtaat tcgcgatctc gctttccagc gtcatattgg ggcgcgctac
    29641 attcgggcgt gggcgtaatt ggtcaatcag gcgcggggtc agcggataaa cattcaccat
    29701 tttgtcgaga tcgaaatcag cgtcctgctg tttgatgacg tctttgccca tcgtcgacat
    29761 attgctgccc ggaagtactg tgcaaccgct tatcaaggtt actgacacca ataatggcat
    29821 caatttcatt ttggatttca tcattgttta tttatcactt tggcagagta attatcctgt
    29881 gcactattaa tagcaatatc gccatgcaca tttaccttgc agttaattga ataaaaattt
    29941 aactggcatc agtcctaaaa aaattgattt catccgcagg ctattgacag aataattcag
    30001 actggtcttt caggcatcca gacacgctac cgcccctggc tttttagcca ccaacacact
    30061 gatttagctt aatttttcaa accctctctg catacaatcg ttgatgagaa agggttattg
    30121 cggaaattaa cttccgaata taaggtgaca ttatggtaat tgaatattgg ctctccaata
    30181 atgcaggagg aagtgttaca gctaacggaa tagcaggcaa ggtaacaatt cggcaattgg
    30241 ctatttttaa gaattatatt aagtgtcatt caatatggtt tttaggagtt tctttaggtt
    30301 gacaatattt aatattgcgc ctccacatgc gatatttctt aaataatgtt ttattattac
    30361 cacttttaat tcagggataa tgtgaggtta ttaccctcaa ataatatgag ataaatagtg
    30421 ctgcaacatt gcatttttgc cccgatttat ccatgatcga attgtgacat ttgtcataca
    30481 acgaataggt tttgtactta ctatggaatg gattgccgat ccgtctatct gggccgggtt
    30541 aatcacgctg attgtgatcg aactggtcct cggcattgat aacctggtct ttattgccat
    30601 cctcgccgaa aaactaccgc cgaggcagcg cgaccgcgca cgggttaccg ggctgctgct
    30661 ggcgatgtta atgcgcctgt tactgctggc gtcaatctcc tggctggtca ccctgactca
    30721 accgctgttc agcttccgct cgtttacctt tagcgcccgc gacttaatca tgctgtttgg
    30781 tggtttcttc ctgttgttca aagccacgat ggagctgaac gaacggctgg aagggaaaga
    30841 cagcaataat cccacacaac gcaaaggtgc gaagttctgg ggcgtggtga cgcaaattgt
    30901 ggtactggac gccattttct cactcgactc agtgattacc gccgtcggga tggtcgacca
    30961 tttactggtc atgatggccg ccgtggttat cgctatcagc ctgatgttga tggccagcaa
    31021 gccgttaacg caatttgtta acagtcaccc gacgatcgtt attctctgct taagcttcct
    31081 gttgatgatt ggctttagcc tggtggcaga aggtttcggc ttcgtcattc cgaaaggcta
    31141 cctgtacgct gccattggtt tctcggtgat gatcgaggcg ctcaatcagt tggctatctt
    31201 taaccggcga cgttttcttt ccgctaacca gacgctgcgc cagcggacta ccgaagcggt
    31261 aatgcgcctg cttagcgggc aaaaagaaga tgcggaactg gacgccgaaa ccgcgtccat
    31321 gctggtggat catggtaacc agcagatctt taatccgcag gaacggcgga tgattgagcg
    31381 ggtacttaat cttaaccagc gtaccgtcag cagcattatg acgtcgcgcc acgatattga
    31441 gcatatcgat ctcaacgcgc cggaagaaga aatccgccag ttgctggagc gaaatcagca
    31501 tacgcggctg gtggttaccg acggtgatga cgcagaagat ttgctcggtg ttgttcacgt
    31561 tatcgacctg ctacaacagt ctctgcgcgg cgaaccgctc aacctgcggg tgttgattcg
    31621 ccagccgctg gtgttcccgg aaaccttgcc gttgttacct gccctggagc agttccgtaa
    31681 tgcccgcacg cactttgctt ttgtggtgga tgagtttggc tcggtggaag ggattgtgac
    31741 attaagtgac gtcactgaaa ccattgtcgg taacttaccg aacgaagtgg aagagatcga
    31801 cgcccgccat gatattcaga agaatgcgga cggttcctgg acggcgaatg gtcatatgcc
    31861 gctggaagat ctggtgcaat atgtgccgct gccgctggat gaaaaacgtg aatatcacac
    31921 cattgccggg ctgttgatgg aatatttaca gcgtattcca aaacctggcg aagaagttca
    31981 ggtgggggat tatttgctta aaacgttgca ggtggaaagc catcgcgtgc agaaggtgca
    32041 gattattccg ctgcataagg atggcgagtt ggagtacgag gtgtgatggt atcggtcatt
    32101 ttacccaata gccgaaagga ataagatgag ggaaccgcgc cagcggcgat ttaccgccgg
    32161 gagcccgggt tgccagggtg gtggcgattg agccaccctg gcacgttcac cggcttgagc
    32221 gttccatagt agcgaggaac atcaagtgaa cggaacagcc ttcgatctta catattcaga
    32281 atataatgat gccgccacga acgcctgatg cgacgcttta cgcgtcttat caggcctaca
    32341 ggactggtca cctggtaacc tacatcttct ccagcaactt cttcacatcc ttgccattgc
    32401 gggaatcttt attccgctcg gcccagtcat tcaggcgacg tttcgcttcg tcctgtagat
    32461 gtttgcgcag taactgatcc acttgcaggc tgtaattgag ttgctgccag ttgccataaa
    32521 cccgcagcgg tactggcgtt tctttcagga aatcaatcag tttactttcc ccgttccagc
    32581 cacccacgac ccgaatatca aactgggtat cgcaggtttg atctgccaga ttcaacatgc
    32641 cttcccctgt cagcgccagc acaggcgatt gaccttgcat gtcgtttaac gtcacgacgc
    32701 catccttcaa cgtcaaatcg gtggtaaagc ggtcaagccg cgtcacgtta tcaaagtttt
    32761 cagcggcctt cacatcaccg ccattacgct ctaccgcttg ctgaatcatc tgctggaagt
    32821 tcatcccttc catgcgcgtg tcggtcattt cgacatgtgc ctgtccttgc cagttgtggc
    32881 ggaatgcgtc ggcatctatg tcagcaccgg agaaatcacc agccagtgac atttttccgg
    32941 tcaacgaaat cggatagtta aacgccttca gaatggtacc aatctcaacg ttttccagcc
    33001 gtggctggaa gtttatccgc ggatttattg atgtcgcgtc cagcgtgccc ggcagtgaaa
    33061 cctgtccacc gttaagtttg ccctgcagtt gagtaatttc cagcaaaccc gacttgttgg
    33121 tcatttgcgt ggcaacatct gtaaaattca ttccgcgcca gcgcacgtta ctggcctgca
    33181 acaaaatatc agccgtaaag ccttgcagtc cctgataggc cggttcatca atacgcgaag
    33241 aaatgaccgg gcgcggcaac gttgattggc tctgcccctg ctgcgcggca ccgttttcac
    33301 cattcgcggt ttcattaagc gggatgaggt tgtcaagatt cagttgcggg aattgcagtc
    33361 tcagctgcca ttccggtttc tctgtcagcg tgacctgtgc ttgcccgctc agcgtactgt
    33421 cattggcggt caagctgatt tggttaaatg aaaggcgttt atgtgactcc tgccactggg
    33481 cctggaaact cccctgcccc tgaatacctt gttttggtaa atcggcaccc tgcaactgcc
    33541 agttaatttg ttcaatagcc gccgttaaat catgcggata atcagaagca tccaccgtac
    33601 cgttaaggga tattgtgaga tcgcgctgat cgcgattaac ccgcccggag aactcaaatg
    33661 agccacgatg ttggggatct tgttccattt gcaggcggat attgcggatt gtcacctgct
    33721 cgtcatcttc atgctggaac accagcacgc tgtccgccac cttaagactg gatatatcaa
    33781 acgaccatcc gcgatcgtct gacagatccg gcaaggtatt gtcgcgcggt gcaaccggag
    33841 cgtcttcact gcgcaccgct tccgtctgcg gcgtcagttg gatcactgcc ccttttagca
    33901 tcacctgctt aacgctcagt tgatgactca gtagtggtaa aagcgccacg tccagacgca
    33961 tgttgtcggc gcgaaccagt ggctggcttg cgccctgggc ggtgagagac attcgcccgg
    34021 agaggatact aagctgcggc cagacgtgcc aacgcagtgg cccgtcgagc tgcaattgat
    34081 aaccgctacg tgcagcaact tgcttgacca tatagtcgcg gaaatcattc ggattcacca
    34141 gcaacactaa cacagataac ccggccacca gcacgaccag gagtatcatc agcgtcgtca
    34201 gaaatcgtct catggtatcc tcaatgggcc tgaattagtc tttatcgatt cggctggcta
    34261 ccgcgccctg ctggttgcga tatttcgcat cttcacggcg gttgtaaggt cgcgccgccg
    34321 ggccggaaag cggctcaaag ctcagcgcac caattaacat gcccggacgc agcgccagcg
    34381 gcagcttacc ggagttgtag aactccagca caatgcaacc agaccagccc ggatcgatac
    34441 ggtgcgcggt gacgtgcacc atcagcccca gacgcgccaa agaggaacgc ccgtccagcc
    34501 agcccaccag atcggctggc agcgtcaccg actccagcgt caccgccagc gccagctctc
    34561 ctgggtgaag atagaacgcc tcgccctcgt cgagaacgat ctcatcgctc atcacgcggt
    34621 caagcgcggc gctcacttca tctttgggac cgctcagatc gataaacgct gccgtgtgac
    34681 cacggaaggt acgaaattta ttgcccaggc gtacatccac cgtcgcgccg ttaatacgct
    34741 ccactggcgg acgtgggttg atcgacaaac ggccttcatc aagccaggct tcaatatctc
    34801 ggtcacacag acgcatggca ttttctcctt tcgcgcatca ctcccttaac gccaattacg
    34861 tcaagggcat accaggttat cactgaacgg tacacaattt atcaagctta ttcaaagaac
    34921 tgacttattt tcgctttcaa tatatcgatc gcgatgcggt ttttcccgcc gcgcggcacg
    34981 ataatgtccg catattgttt agaaggctca atgaattgca ggaacatcgg gcgcacggtt
    35041 ttttgatatt gcgccatcac ggaatccatt gaacgcccac gctcgttaac gtcacgcttg
    35101 atgcggcgca tcaggcagat atccagcggg gtatcaacga aaatggagaa gttaagttcg
    35161 tcacgcaaac gcgcatccgt cagcaacaaa atgccttcga gaatgatgac cttcttcggc
    35221 tcaaccgtca ccgtttcttt catacgcgta tgttcaacat agctgtaaac cggcaggtca
    35281 attgccgagc cgcgtttcaa cgcttgtaaa tgctcaagca gcaggctgtg atccatcgcg
    35341 ctgggatggt cgtagttggt cttaacgcgt tcttccatcg acagatggct ttgatctttg
    35401 taatagcagt cttcgggaat tacgccgatg tgttcatcac cgacttgctc acgtaattca
    35461 cgataaaggg tactggcaat aagactcttg ccggaagccg atgcgccagc gataccgata
    35521 atgacgcact gatgagactg atcagtcata tatttagcga cctgattaac ctggatgtta
    35581 ggaagggggc gacgaagcgc caaacgcggc aattataggg atttcatccg tctgatacca
    35641 gtcgaatagc gttgccgcgc gctcagagtt aattgttgac aaagaattcc cggtggcaaa
    35701 ttacgttgat cagttttatg taaggtaaaa aatgttatac gcagttgcgc aaattatccg
    35761 cctttacgtc actttatgag caattcgcat ataaaatgta aaacttttgt actagcataa
    35821 acacagaaac gaatactggc gacctggtct tgcggataaa gcggtaatga gcaaacaatc
    35881 acagcatgta ttaattgccc tgccccaccc gctgcttcac ctggtcagtt taggtttagt
    35941 ctcgtttatc tttacccttt tctcgcttga gctttcgcag tttggcaccc aactcgcccc
    36001 actgtggttc ccgacgtcca tcatgatggt ggcgttttat cgccatgccg ggcgcatgtg
    36061 gccgggaatt gcgctgagct gttcgctggg aaatatcgcc gcatccatcc tgcttttttc
    36121 caccagctcg ctgaacatga cctggacgac catcaatatt gttgaagccg tggtcggggc
    36181 agtgctgcta cgtaaattgc tgccgtggta taaccccttg caaaatctgg ctgactggct
    36241 gcgtctggca ctcggcagcg ccattgttcc gcctctgttg gggggtgttc tggttgtcct
    36301 gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt cagaatccat
    36361 cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc tgctgcgcca
    36421 tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac tgacgttaag
    36481 ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc tgttgatgtg
    36541 gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca cggtgatgat
    36601 ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta cgtacctgat
    36661 gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca tcatgaccat
    36721 ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa cccgctttcg
    36781 gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg gacaatggct
    36841 gcaaaccaac aaagcgctct gccagtttct cgggtacagt caggaagagc tgcgcggact
    36901 cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac aggttgaaaa
    36961 gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca accgcaatgg
    37021 cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg gcacgccgct
    37081 ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac aggtcaatca
    37141 gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca tctgggagag
    37201 ggagttgaag ccgaatgttt ttagctggga taagcggatg ttcgagctgt atgaaattcc
    37261 tccacatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg aagatcgcca
    37321 gcatgccgaa aaagtgattc gtgattcgtt gcaatcacgc tcgcccttta aactggaatt
    37381 tcgcattacc gtaaaagacg gtattcgcca tatccgcgcc ctcgccaacc gggtactgaa
    37441 taaagaaggc gaagtcgaac gtctcctcgg catcaatatg gatatgaccg aagtgaaaca
    37501 gcttaacgag gcattgtttc aggaaaaaga gcgcctgcac attacgcttg attccatcgg
    37561 tgaagccgtg gtctgtattg atatggcgat gaaaattacc tttatgaatc cagtggcgga
    37621 gaagatgagc ggctggacgc aggaagaagc gttaggtgtt ccgctcctga cggtgttgca
    37681 tattactttt ggcgacaacg gaccattaat ggagaacatt tacagtgccg acacctcacg
    37741 ttccgcaatc gaacaagatg tggtgttgca ctgtcggagc ggcggcagtt acgacgtgca
    37801 ttacagtatt acgccgttaa gtactctgga cggcagcaat attggttcgg ttctggtgat
    37861 tcaggacgtc accgaatcac gcaaaatgct gcgccagctg agctacagcg cctcccatga
    37921 tgcactgacg catctcgcca accgcgccag ttttgagaaa caactgcgta tcctgctgca
    37981 aacggtaaac agtacgcatc agcgacatgc cctggtgttt atcgatcttg atcgctttaa
    38041 agcggtgaat gacagcgccg ggcatgcggc gggcgacgct ttgctgcgcg aactggcgtc
    38101 gttgatgctg agtatgctgc gctccagtga cgtgctggcg cggctcggcg gtgatgaatt
    38161 tggtctgctg ctgccagact gcaatgttga aagcgctcgt tttatcgcta cacgtattat
    38221 cagtgctgtg aatgactatc actttatatg ggaaggccgg gtacatcggg taggtgccag
    38281 cgccgggatt accttgatcg atgacaacaa tcatcaggcg gcagaagtga tgtcgcaggc
    38341 tgatatcgcc tgttatgcct ccaaaaatgg tggacggggc cgggtgacgg tttacgaacc
    38401 acagcaagct gccgcacata gcgaacgggc ggcgatgtcg cttgatgaac agtggcggat
    38461 gattaaagag aatcagttga tgatgatcgc ccacggtgtc gcttcgccgc ggatcccgga
    38521 agcgcgtaat ttgtggctga tttcgcttaa gctctggagt tgcgaaggcg aaattattga
    38581 tgaacaaaca tttcgtcgta gcttcagcga tccggcactt agccatgctc ttgaccgacg
    38641 ggtattccac gaatttttcc agcaggccgc caaagcggtt gccagtaaag gcataagcat
    38701 cgccctcccc ctttccgttg ccggtttgag tagcgccacg ctggtgaatg atctgcttga
    38761 gcagctggaa aatagccctc taccaccacg gttattacat ctgattattc cggctgaagc
    38821 gattttagat cacgcagaaa gcgtgcaaaa actgcagctg gcgggatgtc ggatagtgct
    38881 cagccaggtg ggccgcgatc tgcaaatctt caactcgctg aaagcgaata tggcagatta
    38941 cctgctactt gatggtgagt tatgcgccaa cgtgcagggt aatttgatgg atgagatgct
    39001 gattacgatt attcaggggc acgctcagcg actcgggatg aaaaccatcg ccgggccagt
    39061 cgttttaccc ttagtgatgg atacgctttc tggcatcggc gtcgatctga tttatggtga
    39121 tgtgattgcc gatgcccaac cgctggattt gctggtgaat agcagttatt tcgcgattaa
    39181 ctgagagagc gtttgcccat tgcctgatgc gacgctaacg cgtcttatca tgcctacaaa
    39241 tcgctcattc cccagaccgg ataaggcgct cgcaccgcat ccggcgacca acgtcatgct
    39301 tcgtctggtt gccagccttc cgtataccag atatgcaaca gcgcataaga acgccagggc
    39361 ttccagcgct cggcataacg gcggatttgt gccggtgtca ttcccggaaa tcgccgttta
    39421 atcagataat catccggcag aaaaacatct ttcgcctgcc agccacgcaa agcaaaataa
    39481 ttcgccgtcc agcgcccgat acccggaaaa gtttgcagcg ttttcatcgc ctgctccaca
    39541 tcgcccggaa ttgtcattgg taagctgccc tccagcgccg catttgccag atgaatcagt
    39601 gcctctgccc gtttcaacgg catgcctaac gcttttaatg cctgcgggtc ggctgctgcc
    39661 agccgctgag gcgtggggaa gcagatatac tccggaaaat catccagccg ttcgccataa
    39721 agctgtacca ctttggcagt caattttgcc gccatcgcca cgctcaccag ttggcctaaa
    39781 atcgcccgca caccctgctc aaaagcatca atacagccgg gtaaacgcaa tccgggccgc
    39841 cccgcgccta actttcccaa cgcaccgtta acaatctgcg ggttacattg cagatcaagc
    39901 aggcggctca ttttcgccag acactctgcg gcaacaggtt ctaaacctgc acttaaattt
    39961 atgtgcagag tatggcgggc tatatccgga atagcagtca ctacgccgcg atattcgccc
    40021 accgccagac tacgggcata ataattgtcc gcgaccgttt ccacaccgct caccgcacgg
    40081 gcggcgagaa atcccaacat ccacgaccag tcatacggcg gctgccagtt cagggtatac
    40141 atcgcatctc cttgttaatc cgctttcagc ataaacgtta ttcagacgtt acgctttgct
    40201 ttcatattcc ggttgtcgcg acagcaacat ttcgctaaag tcacgcccct tcttcaccgg
    40261 catggggatt aattcgtgtt tattggtttt gattacggta cagcaaactg ttcagtggcg
    40321 gtcatgcgtg acggtaaacc gcaattactg aaaatggaaa acgacagcac gctgctgcct
    40381 tcaatgcttt gcgcgccaac gcgtgaatcg gtaagcgaat ggctgtaccg ccatcatgat
    40441 gttccggcag acgacgatga aacgcaggcg ctgttacgtc gggcgattcg ttataaccgc
    40501 gaagaagata tcgatgttac ggcgaaaagc gtgcagttcg gtctttcctc actggcacag
    40561 tacattgatg atccagaaga agtgtggttt gtgaaatcac caaaatcgtt cctcggtgcc
    40621 agcggcttaa aaccgcagca ggtagcgctg tttgaggatc tggtctgcgc aatgatgttg
    40681 cacattcgcc agcaggcgca ggcgcagctg ccagaagcga ttactcaggc ggtgattggt
    40741 cgcccgatca acttccaggg gctgggcggc gatgaagcaa acgcccaggc gcaagagatt
    40801 ctggaacgcg cggcgaagcg tgccggattc agggacgtgg tattccagta cgagccgatc
    40861 gcggctgggc tggattacga agccaccttg caggaagaaa aacgggtgct ggtggtggat
    40921 atcggcggtg gtacgactga ctgttcattg ctgctgatgg ggccgcagtg gcgttcgcgt
    40981 ctcgatcgtg aagccagtct gctgggtcac agtggttgtc gtattggcgg taacgatctg
    41041 gatatcgccc tggcgtttaa aaacctgatg ccactgctgg gtatgggtgg cgaaaccgaa
    41101 aaaggcatcg ccctgccgat cctgccgtgg tggaatgcgg ttgccatcaa cgacgtacct
    41161 gcgcagagtg atttctacag tagtgccaac ggtcgtctgc ttaacgatct ggtacgcgat
    41221 gcccgcgaac cggaaaaagt ggccctgtta cagaaagtct ggcgtcagcg tttaagctat
    41281 cgcctggtgc gtagcgcaga agagtgcaaa attgctcttt caagcgtagc ggaaacccgc
    41341 gcctcactgc cgtttatcag caatgaactg gctacgctga ttagccagcg agggctggaa
    41401 agcgccctca gccagccact ggcgcggatt ctggaacagg tgcaactggc cctggataac
    41461 gcccaggaaa aaccggacgt tatctacctg accggcggta gcgcccgttc tccgcagatt
    41521 aaaaaagcgc tggcagaaca gttgccgggc attccgattg caggcggcga tgactttggc
    41581 tccgtcaccg ccgggctggc acgctgggcg gaagtggtgt ttcgttaatt gaaaaatggc
    41641 tcagcagcgg cttaaatctg cctgcgcctg agccagtttt tctgctgcct gacgtaacgg
    41701 ttccatcaac ggcatggtcc gtgctggagc atgaaggcgg aatcgctgca actctgtcgc
    41761 gccgactgtc aacgccgatt ccagccgatg tcgtttggcg gtcattttcg ccattaccgc
    41821 ttgtctgtct gctggcgtta tcgcttcatt cggcctgaac acaaaacggc gttcacagtt
    41881 cgctttccag tcgataaccg cctgggtcag atggtcgcca aaccctttca cttgcttaac
    41941 gccacgacgg gtaacatctg cggctgtttc aataccaaaa gaccgtaacg ccgctttacg
    42001 cgcagggcca acgccgggaa tagaggcaac atcaataaaa aatccctcca gaaacttctg
    42061 cttctgccgt tcccttgcag tgtcatgaag tgctgccaga gcgcgtttct cttcttccgg
    42121 taacccgaga atttcgtcct tcattttttc gagcatcgtc cgtttggcga taaaaccttc
    42181 caggccgcct acctgctgga tctggcggac taaatggtca tagtccattt tcgcacgatt
    42241 aaatgcctca cggcgttgct gaacttctgc tttgtacgct ttgcttgtca aactgccaat
    42301 aatccagata gccgccagca caggaaccaa aataatataa cgcggttctg cctgaaggcc
    42361 gcaaagcagc agcgataatg ctgacagtgc gatctcaagc agaatgatgt attcgcgccg
    42421 taacaggcct aaaggaagcg gcctgccagt cggttggaaa tgatcgggta atggcagttg
    42481 caatgctggc ggtgctactg acgccatcac catcgcccag acttttgcca gcacaaaatc
    42541 cccgccggtg gtaatgacct cttcgccgag atcaataaaa tagataacac cttgattatc
    42601 cagcgcacac catgggcagt cagccaaatg accgggataa acatgcattg ccgaaacggt
    42661 acatttcttt aactgttggc gtagagaatc cagtgctgat acccacgctt tagccgtcgg
    42721 acgcccggtt gccacgccac tttccgtaaa cgcctgctga aacatggctt caacatcact
    42781 cggtaacatc gatagcggaa tcgatcgtgg cggcggtttt aaaccacgtc gctgattatc
    42841 tgacgcgtag gcataacgga aatgggtaat atccgtctcc agcgcattac ccgcatcaga
    42901 gataagcggc acaccagaat aaggatgccg gccaccaaac aagacgtgaa aaatcagcaa
    42961 cgcaaggcca aaattatcgt gattttctgt gcgttcaaag ccgacaaatg acgacaaggt
    43021 ttgtagctct ggcggcgtaa aatgcgacac gccgacttcg cataaatgca gtgtgccatt
    43081 ggcgttaatc tgaaaggagt cgctatcaat caacaccact ttgctgtcgc gacctaccat
    43141 aaaactgttc tggtttacgt cacccacgac gtgcccgtgc tcgtgaaccg tagcaaaaga
    43201 tgaagcaata ttgcgcgcaa catagagtag aaaatcccac gcacaatgag ggtaacgttg
    43261 acgacgatgt gccgggctat agatcatatg aatcggttct ttaccagaaa cttttggcat
    43321 cataaaaccg ataacttttc cgcctcgtcc accgtgaagc gttgcctgcg gccaggcgac
    43381 ataattcaac aattgcgcgt cagctgtcgc agccataaag gcaagtttgt cctgttttaa
    43441 ggcgggtggc ggcgtgtgat aaatcttggc gacgctatcg acaaactcat tgatatcata
    43501 aaccgcgcct tcaccgcctt tgcccagttc acggcctggc gtaacgcact caccagtagc
    43561 agtatataaa gtgggtttca tggtaaccac agcgccagtg ccaacgtttt atcatcatcc
    43621 gtacgctcgt ttaccgccgg gctggacaaa aactgcttta acaattcagg taataaatcg
    43681 agttgttcct gcgttgctga cgccagtcca ttgaaaaacg gggtaaagaa aggcacatga
    43741 ggagaattat ccagcatatt taacgccagc cgctgaatac catccgtaaa tgctgcaact
    43801 ttatgcgcac gcccagtgct ggtgaaagtt tccagtctgg aaacggcatc ttcgtcggta
    43861 atgaagtgcg tcatgttggc atattcaccc accatcggcg tgagaggcaa ttgcagaccg
    43921 tgacccaaat ctaccaccac gccgccatcg ccaatctgca taatcaaggt gccgtcaggc
    43981 gatgaaatca atcccagaaa cgtgcaggca aaatcgcgaa ctgctaatgc tttggcttct
    44041 gcctcggcaa aaagtcgttg acgaatagtc agtaccatat ttgtggcgag gacatcatta
    44101 agccccaatt caccgtcctg tactttttgc accataaacg ccatcgcttc attgacggcg
    44161 agcgtcgcgc cttctcctcc ctgcgaaacg ctacctgcgc catcagcaag gaacatcacc
    44221 agtaatggtt gctggtcgtt taaccagaca acctgcatct ggcaggcatc ctgacaaggc
    44281 aaatcagcac taatgtgcga tgttccaaca gcggatgcat aaaccagacg ccagctcaca
    44341 ctgacgtcca gccttttggc gcttccagca caacttccgt tcccggcgtg gagcgggaaa
    44401 ccgaacgcag cgaactggat aaccagctaa acagttcacg gaattgtaat ccctgcagtg
    44461 gcaaaggctg acgaacgctg atttgtgcca gagtcttcat atccgccccc tgaacaccga
    44521 tggaaaagaa ggcaaacttc ttatcttctt ccccctgaaa cactttgttg gcggcagcct
    44581 gccactcatc ggttggagcg ccatcggtga tcaggaaaat ccacgggcga taatacgaaa
    44641 taccatttgc gcggtactca cgtttcctct cttcaaccat atccagggct ttggtaatgg
    44701 cggcccccat aggcgtatcc ccctgggcaa acaggatggg cgggaaaaag ttagcggcgc
    44761 tggtaaaggg ttgttccaca tgcaccgggc cgaacgtcac aatgccgagt tccactcttt
    44821 ttaaagccag cggatcagca agcagttcat cacgaaaggt aaccaatccg gcgttaagtt
    44881 cattaatagg tctgccgctc atcgagccag aaacatccag cagcaaaatg caggggcaac
    44941 gtggttccgg gttactggca aaatcgctgg tggcaaacgt gatttgttcg ctcataagaa
    45001 atcctttctt agagaattat cgaggtccgg tatcataccg attgcaataa tgaaataaga
    45061 gcaaacgaag aaactttcag aattaggccc gtcggcgcag aaaattttgc tattcgcctc
    45121 gcaagaacgg agagccgtcg attgacacta agggcggagt gacataattt caggagtgag
    45181 ggttagggag aggtttcccc ctccccctgg tgttcttagt aagcctggaa gctaatcact
    45241 aagagtatca ccagtatgat gacgtgcttc atcataaccc tttccttatt aaaagccctc
    45301 ttctccggga gagggtttcc cgtttcagcg tcccgctgaa atcgtcggct tacctccttt
    45361 cgccctgaat gcagtctatc gctaacgcgt gaacaaaata gtttcatctg tgattattgc
    45421 gtaacgactc gcaaagaata tttgctggca ggatcgcaga ctacaaagcc tgcggattga
    45481 caatcttatc gtgaaggcat aatttcagga gtgagggtag agcggggttt cccccgccct
    45541 ggtagtctta gtaagcgggg aagcttatga ctaagagcac cacgatgatg agtagcttca
    45601 tcatgaccct ttccttatta aggccccttc ctcgggaggg gctttcccgt ttcagcgtcc
    45661 cgctgaaatc gtcggcttac ctcctttcgc cctgtatgca gtctatcgct gacttgcgga
    45721 taaaatagtt tccagtgtga ttattgcgta acgtctcgca aagaatattt gctggcagga
    45781 tcgcagactg caaagcctgc agattgacaa tcttatcgtg aaggcatact ttcaggagtg
    45841 agggtagagc ggggtttccc ccgccctggt agccttagta agctggccag ctaatgacta
    45901 agagcaccac gatgatgagt agcttcatca tgaccctttc cttatttatg gccccttcct
    45961 cgggaggggc tttcccgttt cagcgtcccg ctgaaatcgg cggcttacct cctttcgcca
    46021 tgcaagcagt ctatcgctaa cgcgtagata aaatagtttc ctgtgttatt actggatgcg
    46081 tgctcgcaaa tgtgcccgtc attcagacga ttcccgacag tgtttcataa ttcctccatt
    46141 tttctccctt attggctggc tacactagta tcattccgcg aaacgtttca ggaagagaaa
    46201 ctcttaacga tgaaaggcag ttataaatcc cgttgggtaa tcgtggtggt tatcgccgcc
    46261 atcgccgcat tctggttctg gcaaggccgc aatgactccc agagtgcagc cccaggggcg
    46321 acgaaacaag cgcagcaatc gccagcgggt ggtcgccgtg gtatgcgttc cggcccatta
    46381 gccccggttc aggcggcgac cgccgtagaa caggcagttc cgcgttacct caccgggctt
    46441 ggcaccatta tcgccgctaa tactgttacg gtgcgcagcc gcgtggacgg tcaactgatg
    46501 gcgttacatt tccaggaagg ccagcaggtc aaagcaggcg atttactggc agaaattgac
    46561 cccagccagt tcaaagttgc attagcacaa acccagggcc aactggcaaa agataaagcc
    46621 acgcttgcta acgcccgccg tgatctggcg cgttatcagc aactggcaaa aaccaatctc
    46681 gtctcccgtc aggagctgga tgcccaacag gcgctggtca gtgaaaccga aggcaccatt
    46741 aaggctgatg aagcaagcgt cgccagcgcg cagttgcaac tcgactggag ccgcatcacc
    46801 gcaccagtcg atggtcgcgt tggtctcaag caggttgatg ttggtaacca aatctccagt
    46861 ggtgatacca ccggaattgt ggtgatcacc cagacgcatc ctatcgattt agtctttacc
    46921 ctgccggaaa gcgatatcgc tactgtagtg caggcgcaaa aagccggaaa aacgctggtg
    46981 gtagaagcct gggatcgcac caattcgaag aaattaagtg aaggcacgct gttaagcctc
    47041 gataaccaaa tcgatgccac aaccggtacg attaaagtga aagcacgctt taataatcag
    47101 gatgatgcgc tgtttcccaa tcagtttgtt aacgcgcgca tgttagtcga caccgaacaa
    47161 aacgccgtag tgatcccaac agccgccctg caaatgggca atgaaggcca ttttgtctgg
    47221 gtgctgaata gcgaaaacaa ggtcagcaaa catctggtga cgccgggcat tcaggacagt
    47281 cagaaagtgg tgatccgtgc aggtatttct gcgggcgatc gcgtggtgac agacggcatt
    47341 gatcgcctga ccgaaggggc gaaagtggaa gtggtggaag cccagagcgc caccactccg
    47401 gaagagaaag ccaccagccg cgaatacgcg aaaaaaggag cacgctcctg atgcaggtgt
    47461 tacccccgag cagcacaggc ggcccgtcgc gcctgtttat tatgcgtcct gtcgccacca
    47521 cgctgctgat ggtggcgatc ttactcgccg ggattatcgg ttatcgcgcc ctgcccgttt
    47581 cggcgctgcc ggaagtggac tatccgacca ttcaggtggt cacgctctac ccaggtgcca
    47641 gcccggatgt catgacctct gccgttaccg cgccgctcga acgccagttc gggcagatgt
    47701 ctggcctgaa acagatgtcg tcgcaaagtt ccggcggtgc gtcagttatc actttgcagt
    47761 tccagctaac attaccgctc gatgtcgccg agcaggaagt gcaggccgcg attaacgctg
    47821 cgaccaactt gttgccgagc gatctgccta acccgccggt ttacagcaaa gtgaacccgg
    47881 cagatccgcc gatcatgacg ctcgccgtca cctcaaccgc catgccgatg acgcaagtgg
    47941 aagatatggt ggaaacccgc gtcgcgcaga aaatctcgca gatttccggc gtcggcctgg
    48001 tgacgctttc cggcggtcag cgtccggctg ttcgcgtcaa acttaacgct caggcgattg
    48061 ccgccctcgg cctgaccagc gaaaccgtgc gcaccgccat taccggcgct aacgttaact
    48121 cggcaaaagg tagcctcgac ggcccttccc gtgcggtcac gctttccgcg aacgaccaga
    48181 tgcaatccgc cgaagagtat cgccagctaa tcatcgccta ccagaacggc gcgccaattc
    48241 gtctgggcga tgtcgcaact gtagagcaag gtgcagaaaa cagctggctc ggcgcgtggg
    48301 cgaacaaaga acaggccatt gtgatgaatg ttcagcgcca gcccggtgct aacattatct
    48361 ccaccgccga cagcattcgg cagatgctgc cacagctcac tgagagtctg ccgaaatcgg
    48421 tgaaggtgac agtgctttcc gatcgcacca ccaatatccg cgcatccgtc gatgatactc
    48481 agtttgaatt gatgatggct atcgcgctgg tagtcatgat tatctacctg tttttgcgca
    48541 atattccggc gaccatcatt cccggtgttg ctgtaccgct gtcgttaatc ggcactttcg
    48601 cggttatggt gtttctcgat ttttcaatca ataacctgac actgatggcg ttaactatcg
    48661 ccaccggatt cgtggtcgat gacgccatcg tggtgatcga aaacatttcc cgctatatcg
    48721 aaaaaggcga aaaaccgttg gcggcggcgc tcaagggcgc aggtgaaatc ggctttacca
    48781 ttatctcgct gaccttctca ctgattgcgg tgttgatccc actgctgttt atgggcgata
    48841 tcgtcgggcg actgttccgc gaatttgcta ttaccctggc ggtagcgatt ttgatctcag
    48901 cggtggtgtc gctgaccctg acaccgatga tgtgcgcgcg gatgctcagc caggagtcgt
    48961 tgcgtaaaca gaaccgcttc tcccgtgcct cggaaaaaat gttcgacagg ataatcgccg
    49021 cctatggtcg tggactggcg aaagtgctga atcatccgtg gctgacctta agcgtggcac
    49081 tcagcacgct gctgcttagc gtgctgctgt gggtgttcat tccgaaaggt ttcttcccgg
    49141 tacaggacaa tggcattatt cagggcactt tgcaggcacc gcaatccagc tcctttacca
    49201 atatggccca gcgacaacgc caggtcgcgg acgtgatttt gcaggatccg gcagtgcaaa
    49261 gcctgacctc atttgttggc gttgatggca ctaacccgtc gctgaatagt gcacgtttac
    49321 aaatcaacct caaaccgttg gatgaacgtg atgatcgggt gcaaaaagtc atcgcccgtc
    49381 tgcaaacggc ggtagataaa gtgccgggcg tcgatctctt cctgcaacca acgcaggatc
    49441 tgactattga tactcaggtc agccgcaccc agtaccagtt taccttgcag gccacctcac
    49501 tggatgcgct cagtacctgg gtgccacagt tgatggaaaa actccagcaa ctgccacagc
    49561 tttctgatgt ctccagcgac tggcaggacc aagggctggt ggcgtatgtc aatgttgatc
    49621 gcgacagcgc cagccgtctg gggatcagca tggcggatgt cgataacgcc ctgtacaacg
    49681 cgtttggtca gcgactgatt tccactattt atactcaggc taaccagtat cgcgtagtgc
    49741 tggagcacaa caccgaaatt accccgggcc tcgcggcgct ggataccatt cgcctgacca
    49801 gtagcgacgg cggcgtggtg ccgttaagct caattgccaa agttgaacag cgttttgcgc
    49861 cgctttccat caaccatctg gatcagttcc cggtaacgac catctccttt aacgtgccgg
    49921 ataactattc gctgggcgat gcggtgcagg agattatgga caccgaaaag acgctgaatc
    49981 tgccggtgga tatcaccacg caattccagg gcagcaccct cgccttccag tcggcgctgg
    50041 gcagcactgt ctggctgatt gtcgcggcgg tggtggcgat gtatatcgtg ctcggcattc
    50101 tgtacgagag ctttattcac ccgatcacca ttctctcgac gctacccacc gcaggggtcg
    50161 gcgcgctgct ggcattaatg attgccggta gcgaactgga tgtgattgcg attatcggca
    50221 ttattttgct gatcggtatt gtgaagaaga acgccatcat gatgatcgac ttcgcgctgg
    50281 ctgctgagcg cgagcaaggc atgtcgccgc gcgatgcaat ctaccaggct tgtctgttgc
    50341 gttttcgtcc gatcctgatg accaccctgg cggctctgct tggcgcgctg ccgctgatgt
    50401 tgagtaccgg ggtcggcgcg gaactgcgtc gtccgttagg tatcggcatg gtcggcggtc
    50461 tgattgtcag ccaggtgctg acgctgttca ccacgccggt gatttatctg ctgttcgacc
    50521 gcctggcatt gtggaccaaa agtcgctttg ctcgccatga agaggaggcg taagtgaagt
    50581 tttttgccct cttcatttac cgcccggtgg cgacgatttt actgtcggtc gccattaccc
    50641 tgtgcggcat actgggcttc cgtatgctgc cggtcgcccc gctgccgcag gtcgattttc
    50701 cggtgattat ggtcagcgcc tcgctgcccg gtgcgtcacc agaaacaatg gcgtcttccg
    50761 ttgccacgcc gctggagcgc tcgcttgggc gcattgccgg ggtcagcgaa atgacctcca
    50821 gcagttcgct cggcagcaca cgtattattt tacagtttga ttttgaccgg gacatcaacg
    50881 gcgcagcgcg tgatgtgcag gcggcgatca acgctgcaca aagtttgctg cccagtggga
    50941 tgcccagccg tccgacctat cgcaaagcga acccgtcgga tgcgccaatt atgatcctca
    51001 cgctgacatc cgatacttat tcgcagggtg aactgtacga tttcgcctcg acgcagctgg
    51061 ctccgacgat ttcgcaaatc gacggtgttg gtgatgtcga tgtcggaggc agctcactgc
    51121 ccgccgtacg cgtcgggctg aatccgcagg cgctgtttaa tcagggcgtg tcgctggacg
    51181 acgtacgcac cgccatcagc aatgccaacg tgcgtaaacc gcagggcgcg ctggaagatg
    51241 gcactcaccg ctggcagatc cagaccaatg atgagctaaa aaccgccgct gaatatcagc
    51301 cgttgattat tcactacaac aacggcggcg cggttcgtct gggcgatgtg gcgacggtga
    51361 ccgactcagt gcaggatgtg cgcaacgccg ggatgaccaa cgccaaaccg gctattttac
    51421 tgatgatccg caaactgccg gaagccaata ttatccagac ggttgacagc atccgggcaa
    51481 aattaccgga gttgcaggaa accattccgg cggcgattga tctgcaaatt gcccaggatc
    51541 gctcccccac cattcgcgcc tcgctggaag aagtcgagca aacgctgatt atctcggtgg
    51601 cgctggtgat tctggtggtg tttttattcc tgcgctcggg tcgcgccact attattcccg
    51661 ccgtttcggt gccggtttcg ctgattggta cgtttgcggc gatgtacctg tgcggattca
    51721 gtctcaataa cctttcgtta atggcgctca ccatcgctac tggtttcgtg gtggatgacg
    51781 ccatcgtggt gctggaaaac attgcacgtc atctggaagc gggaatgaaa ccgttgcaag
    51841 ccgcactgca aggtactcgc gaagtcggtt ttacggtgct gtcgatgagt ctgtcactgg
    51901 tggcggtgtt cctgccgctg ctgttgatgg gcggattgcc gggccgactg ttacgcgagt
    51961 ttgccgtgac gctttctgtc gccattggta tttcgttgct ggtttctctg acattaacgc
    52021 caatgatgtg tggctggatg ctgaaagcca gcaagccgcg cgagcaaaag cgactgcgtg
    52081 gttttggtcg catgttggta gccctgcaac aaggctacgg caagtcactg aaatgggtgc
    52141 tcaatcatac ccgtctggtg ggcgtggtgc tgcttggcac cattgcgctg aatatctggc
    52201 tgtatatctc gatcccgaaa accttcttcc cggagcagga cactggcgtg ttgatgggcg
    52261 ggattcaggc ggatcagagt atttcgtttc aggcgatgcg cggtaagttg caggatttca
    52321 tgaaaattat ccgtgacgat ccggcagtgg ataatgtcac cggctttaca ggcggttcgc
    52381 gagtgaacag cgggatgatg tttatcaccc tcaagccacg cgacgaacgc agcgaaacgg
    52441 cgcagcaaat tatcgaccgt ctgcgcgtaa aactggcgaa agaaccgggg gcgaatctgt
    52501 tcctgatggc ggtacaggat attcgcgttg gtgggcgtca gtcgaacgcc agctaccagt
    52561 acacgttgtt atccgacgac ctggcggcac tgcgcgaatg ggagccgaaa atccgcaaaa
    52621 aactggcgac gttgccggaa ctggcggacg tgaactccga tcagcaggat aacggcgcgg
    52681 agatgaatct ggtttacgac cgcgacacca tggcacggct gggaatcgac gtgcaggccg
    52741 ccaacagcct gttaaataac gccttcggtc agcggcaaat ctcgaccatt taccagccga
    52801 tgaaccagta taaagtggtg atggaagtgg atccgcgcta tacccaggac atcagtgcgc
    52861 tggaaaaaat gttcgttatc aataacgaag gcaaagcgat cccgctgtcg tatttcgcta
    52921 aatggcaacc ggcgaacgct ccgctgtcgg tgaatcatca gggattatcg gcggcctcga
    52981 ccatctcgtt caacctgccg accggaaaat cgctctcgga cgccagtgcg gcgatcgatc
    53041 gcgcaatgac ccagcttggt gtgccttcga cggtgcgcgg cagttttgcc ggaacagcgc
    53101 aggtgttcca ggagacgatg aactcacagg tgatcctgat tatcgccgcc atcgccacgg
    53161 tgtatatcgt gctgggtatc ctttacgaga gttacgtaca cccactgacg attctctcca
    53221 ccctgccctc ggcgggcgtt ggtgcgctgc tggcgctgga gctgttcaat gccccgttca
    53281 gcctaatagc cctgataggg atcatgctat taatcggcat cgtaaagaaa aacgccatta
    53341 tgatggtcga ttttgcgctc gaagcccaac ggcacggtaa cctgacgccg caggaagcta
    53401 ttttccaggc ctgtctgctg cgttttcgcc cgattatgat gactaccctg gcggcgctgt
    53461 ttggcgcgct gccgctggtg ttgtcgggcg gtgacggctc ggagctgcgg cacctcctgg
    53521 ggatcaccat tgtcggtgga ctggtaatga gccagcttct gacgctgtat accacgccgg
    53581 tggtgtatct ctttttcgac cgtctgcggc tgcgtttttc gcgtaaacct aaacaaacgg
    53641 taaccgagta aatgacagaa cttcccgaca gcacccgctg gcaattgtgg attgtggctt
    53701 tcggcttctt tatgcagtcg ctggacacca ccatcgttaa caccgccctt ccctcaatgg
    53761 cgcaaagcct cggggaaagt ccgttgcata tgcacatggt cattgtctct tatgtgctga
    53821 ccgtggcggt gatgctgccc gccagcggct ggctggcgga caaagtcggc gtgcgcaata
    53881 ttttctttac cgccatcgtg ctgtttactc tcggttcact gttttgcgcg ctttccggca
    53941 cgctgaacga actgttgctg gcacgcgcgt tacagggcgt tggcggtgcg atgatggtgc
    54001 cggtcggcag gttgacggtg atgaaaatcg taccgcgcga gcaatatatg gcggcgatga
    54061 cctttgtcac gttacccggt caggtcggtc cgctgctcgg tccggcgctc ggcggtctgc
    54121 tggtggagta cgcgtcgtgg cactggatct ttttgatcaa cattccggtg gggattatcg
    54181 gtgcgatcgc cacattgctg ttaatgccga actacaccat gcagacgcgg cgctttgatc
    54241 tttccggctt tttattgctg gcggttggca tggctgtatt aaccctggcg ctggacggca
    54301 gtaaaggtac aggtttatcg ccgctggcga ttgctggcct ggtcgcagtt ggcgtggtgg
    54361 cactggtgct ttatctgctg cacgccagaa ataacaaccg cgccctgttc agtctgaaac
    54421 tgttccgcac tcgtaccttt tcgctgggcc tggcggggag ctttgccgga cgtattggca
    54481 gtggcatgtt gccctttatg acaccggttt tcctgcaaat tggcctcggt ttctcgccgt
    54541 ttcatgccgg actgatgatg atcccgatgg tacttggcag catgggaatg aagcgaattg
    54601 tggttcaggt ggtgaatcgc tttggttatc gtcgggtact ggtagcgacc acgctgggtc
    54661 tgtcgctgat caccctgttg tttatgacta ccgctctgtt gggctggtac tacgttttgc
    54721 cgttcgtcct gtttttacaa gggatggtca actcgacccg tttctcctcc atgaacaccc
    54781 tgacgctgaa agatctcccg gacaatctgg cgagcagcgg caacagtctg ctgtcgatga
    54841 ttatgcaatt gtcgatgagt atcggcgtca ccatcgccgg gctgttgctg ggactttttg
    54901 gttcgcagca tgtcagcgtc gacagcggca ccacacaaac cgtctttatg tacacctggc
    54961 ttagcatggc gtttatcatc gcccttccgg cgttcatctt tgccagagtg ccgaacgata
    55021 cgcatcaaaa tgtagctatt tcgcggcgaa aaaggagcgc gcaatgaagt tctggcgacc
    55081 tggtattacc ggcaaactgt ttctggcgat tttcgccacc tgcattgttt tgctgatcag
    55141 tatgcactgg gcggtgcgta tcagttttga gcgtggcttt attgattaca tcaagcatgg
    55201 taatgaacag cgattacaac tgttaagtga tgcgcttggc gagcagtatg cgcagcatgg
    55261 caactggcgc ttcctgcgca acaatgatcg ctttgtcttt cagatcctgc gttcatttga
    55321 acacgataat tcggaagata aacccggccc gggtatgcca ccgcacggct ggcgtaccca
    55381 gttctgggtg gttgatcaaa acaacaaagt gctggttggt ccgcgagcgc cgattccacc
    55441 tgacggtaca cggcgaccca ttctggtcaa cggtgcggaa gttggcgcgg tgatcgcctc
    55501 ccccgttgag cggttaacgc gcaatactga tatcaatttc gataaacaac agcggcaaac
    55561 cagctggttg attgtcgccc tggcaacgtt actcgcggca cttgccactt ttctgctggc
    55621 gcgcggttta ctggcaccgg taaaacgact tgtcgatggc acgcacaaac tggcggcggg
    55681 cgatttcact acccgcgtaa cgcccaccag tgaagatgaa ctgggcaaac tggcgcaaga
    55741 cttcaaccag cttgccagca cactggagaa aaaccagcaa atgcggcgcg attttatggc
    55801 cgatatttct cacgaactgc gtacgccatt agcggtgctg cgcggtgaac tggaagctat
    55861 tcaggatggc gtgcgcaaat tcacgccgga gacggtggcg tctttacagg cggaggtcgg
    55921 tacactgacc aaactggttg acgatctcca tcagttgtcg atgtctgatg aaggcgccct
    55981 cgcctatcaa aaagcaccgg tagatttgat cccactgctg gaagtggcgg gcggcgcatt
    56041 tcgcgaacga ttcgccagcc gtggcctgaa actgcaattt tccctgccag acagtattac
    56101 cgtatttggc gatcgcgacc gtttaatgca gttattcaat aatttacttg aaaacagcct
    56161 gcgctacact gacagcggcg gcagcctgaa aatctctgcc gagcagcacg acaaaacggt
    56221 gcgcctgacc tttgccgaca gtgcgccagg tgtcagtgac gagcagctac aaaaattatt
    56281 tgaacgtttt tatcgcaccg aaggttcccg caaccgcgcc agcggtggtt ccgggctggg
    56341 gctggcgatt tgcctgaaca ttgttgaagc acataatggc cgcattattg ctgcccattc
    56401 gccttttggc ggggtaagca ttacagtaga gttaccgctg gaacgggatt tacagagaga
    56461 agtatgaccg agttaccaat cgacgaaaac acaccgcgta ttttgatcgt ggaagatgaa
    56521 ccgaagctgg ggcagttgct cattgattat ctgcgtgctg cgagctatgc gccgacgctt
    56581 atcagccacg gcgatcaggt actgccgtat gtgcgccaga caccaccgga tctgatcctg
    56641 ttggatctga tgctccctgg caccgatggc ttgacgctgt gccgggaaat tcgtcgtttt
    56701 tctgacattc ctatcgtgat ggtgacggca aaaatcgaag agatcgaccg cctgctcgga
    56761 ctggagattg gcgcagatga ctacatctgc aagccgtaca gcccacggga agtggtagcg
    56821 cgcgtcaaaa ccattttgcg ccgttgcaaa ccgcagcgcg aattgcagca acaggatgcc
    56881 gaaagcccgt tgattatcga cgaaggtcgt tttcaggctt catggcgcgg taaaatgctt
    56941 gacctgacgc ctgcggaatt tcgtctgttg aaaacgctct ctcatgaacc aggaaaagtg
    57001 ttctcccgcg agcaattgct caatcatctt tatgacgact accgcgtagt aaccgaccgc
    57061 accattgaca gccacattaa aaacctgcgc cgcaagctgg aatctctcga cgccgaacag
    57121 tcatttatcc gcgccgttta cggcgttggt taccgctggg aagccgacgc ctgccgcatc
    57181 gtttagtttt agcgacatga ctttgttagc cggagatggc gttcagacca gatccggcaa
    57241 cattatccca cgcatggtca gcaaactga
//
LOCUS       DAZPAC010000003        54291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZPAC010000003 DAZPAC010000000
VERSION     DAZPAC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54291
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.003"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            140..1378
                     /gene="preT"
                     /locus_tag="ACHUPM_000119"
                     /pseudo
     CDS             140..1378
                     /gene="preT"
                     /locus_tag="ACHUPM_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            1372..2607
                     /gene="preA"
                     /locus_tag="ACHUPM_000120"
     CDS             1372..2607
                     /gene="preA"
                     /locus_tag="ACHUPM_000120"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFR0868026.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNTDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(2734..3744)
                     /gene="mglC"
                     /locus_tag="ACHUPM_000121"
     CDS             complement(2734..3744)
                     /gene="mglC"
                     /locus_tag="ACHUPM_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR0868027.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(3760..5280)
                     /gene="mglA"
                     /locus_tag="ACHUPM_000122"
     CDS             complement(3760..5280)
                     /gene="mglA"
                     /locus_tag="ACHUPM_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR0868028.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(5341..6339)
                     /gene="mglB"
                     /locus_tag="ACHUPM_000123"
     CDS             complement(5341..6339)
                     /gene="mglB"
                     /locus_tag="ACHUPM_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR0868029.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(6619..7617)
                     /gene="galS"
                     /locus_tag="ACHUPM_000124"
     CDS             complement(6619..7617)
                     /gene="galS"
                     /locus_tag="ACHUPM_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR0868030.1"
                     /translation="MATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQV
                     SDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRC
                     NALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNN
                     GHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELL
                     GRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRY
                     PIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM"
     gene            complement(7801..8958)
                     /gene="yeiB"
                     /locus_tag="ACHUPM_000125"
     CDS             complement(7801..8958)
                     /gene="yeiB"
                     /locus_tag="ACHUPM_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR0868031.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(8975..9643)
                     /gene="folE"
                     /locus_tag="ACHUPM_000126"
     CDS             complement(8975..9643)
                     /gene="folE"
                     /locus_tag="ACHUPM_000126"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR0868032.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            9901..10737
                     /gene="yeiG"
                     /locus_tag="ACHUPM_000127"
     CDS             9901..10737
                     /gene="yeiG"
                     /locus_tag="ACHUPM_000127"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR0868033.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(10769..12760)
                     /gene="cirA"
                     /locus_tag="ACHUPM_000128"
     CDS             complement(10769..12760)
                     /gene="cirA"
                     /locus_tag="ACHUPM_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFR0868034.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(13052..14521)
                     /gene="lysP"
                     /locus_tag="ACHUPM_000129"
     CDS             complement(13052..14521)
                     /gene="lysP"
                     /locus_tag="ACHUPM_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR0868035.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(14726..15607)
                     /gene="yieE"
                     /locus_tag="ACHUPM_000130"
     CDS             complement(14726..15607)
                     /gene="yieE"
                     /locus_tag="ACHUPM_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR0868036.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            15706..16755
                     /locus_tag="ACHUPM_000131"
     CDS             15706..16755
                     /locus_tag="ACHUPM_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR0868037.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            16829..17686
                     /gene="nfo"
                     /locus_tag="ACHUPM_000132"
     CDS             16829..17686
                     /gene="nfo"
                     /locus_tag="ACHUPM_000132"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR0868038.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            17690..18778
                     /locus_tag="ACHUPM_000133"
     CDS             17690..18778
                     /locus_tag="ACHUPM_000133"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR0868039.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(18834..20084)
                     /locus_tag="ACHUPM_000134"
     CDS             complement(18834..20084)
                     /locus_tag="ACHUPM_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0868040.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(20184..21125)
                     /gene="rihB"
                     /locus_tag="ACHUPM_000135"
     CDS             complement(20184..21125)
                     /gene="rihB"
                     /locus_tag="ACHUPM_000135"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR0868041.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            21255..21953
                     /locus_tag="ACHUPM_000136"
     CDS             21255..21953
                     /locus_tag="ACHUPM_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFR0868042.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLLDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(22020..23270)
                     /locus_tag="ACHUPM_000137"
     CDS             complement(22020..23270)
                     /locus_tag="ACHUPM_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0868043.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(23364..24302)
                     /gene="psuG"
                     /locus_tag="ACHUPM_000138"
     CDS             complement(23364..24302)
                     /gene="psuG"
                     /locus_tag="ACHUPM_000138"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR0868044.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(24290..25231)
                     /locus_tag="ACHUPM_000139"
     CDS             complement(24290..25231)
                     /locus_tag="ACHUPM_000139"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR0868045.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(25689..27380)
                     /gene="fruA"
                     /locus_tag="ACHUPM_000140"
     CDS             complement(25689..27380)
                     /gene="fruA"
                     /locus_tag="ACHUPM_000140"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR0868046.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(27397..28335)
                     /gene="fruK"
                     /locus_tag="ACHUPM_000141"
     CDS             complement(27397..28335)
                     /gene="fruK"
                     /locus_tag="ACHUPM_000141"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR0868047.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(28335..29465)
                     /gene="fruB"
                     /locus_tag="ACHUPM_000142"
     CDS             complement(28335..29465)
                     /gene="fruB"
                     /locus_tag="ACHUPM_000142"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR0868048.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            29833..31014
                     /gene="setB"
                     /locus_tag="ACHUPM_000143"
     CDS             29833..31014
                     /gene="setB"
                     /locus_tag="ACHUPM_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR0868049.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(31011..31265)
                     /locus_tag="ACHUPM_000144"
     CDS             complement(31011..31265)
                     /locus_tag="ACHUPM_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR0868050.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            31420..31992
                     /gene="yeiP"
                     /locus_tag="ACHUPM_000145"
     CDS             31420..31992
                     /gene="yeiP"
                     /locus_tag="ACHUPM_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR0868051.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            32215..33681
                     /locus_tag="ACHUPM_000146"
     CDS             32215..33681
                     /locus_tag="ACHUPM_000146"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0868052.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            33799..34785
                     /gene="yeiR"
                     /locus_tag="ACHUPM_000147"
     CDS             33799..34785
                     /gene="yeiR"
                     /locus_tag="ACHUPM_000147"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR0868053.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            34824..35537
                     /gene="lpxT"
                     /locus_tag="ACHUPM_000148"
     CDS             34824..35537
                     /gene="lpxT"
                     /locus_tag="ACHUPM_000148"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR0868054.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            35949..36515
                     /gene="mepS"
                     /locus_tag="ACHUPM_000149"
     CDS             35949..36515
                     /gene="mepS"
                     /locus_tag="ACHUPM_000149"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR0868055.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            36696..38252
                     /locus_tag="ACHUPM_000150"
     CDS             36696..38252
                     /locus_tag="ACHUPM_000150"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR0868056.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            38334..40148
                     /locus_tag="ACHUPM_000151"
     CDS             38334..40148
                     /locus_tag="ACHUPM_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR0868057.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            40149..41243
                     /locus_tag="ACHUPM_000152"
     CDS             40149..41243
                     /locus_tag="ACHUPM_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR0868058.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            41243..42268
                     /locus_tag="ACHUPM_000153"
     CDS             41243..42268
                     /locus_tag="ACHUPM_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0868059.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            42270..43859
                     /gene="yejF"
                     /locus_tag="ACHUPM_000154"
     CDS             42270..43859
                     /gene="yejF"
                     /locus_tag="ACHUPM_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR0868060.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(43863..44207)
                     /locus_tag="ACHUPM_000155"
     CDS             complement(43863..44207)
                     /locus_tag="ACHUPM_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR0868061.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(44540..45597)
                     /locus_tag="ACHUPM_000156"
                     /pseudo
     CDS             complement(44540..45597)
                     /locus_tag="ACHUPM_000156"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(45688..46383)
                     /gene="rsuA"
                     /locus_tag="ACHUPM_000157"
     CDS             complement(45688..46383)
                     /gene="rsuA"
                     /locus_tag="ACHUPM_000157"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR0868062.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            46533..48293
                     /locus_tag="ACHUPM_000158"
     CDS             46533..48293
                     /locus_tag="ACHUPM_000158"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR0868063.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            48418..48702
                     /gene="rplY"
                     /locus_tag="ACHUPM_000159"
     CDS             48418..48702
                     /gene="rplY"
                     /locus_tag="ACHUPM_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR0868064.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(48841..49848)
                     /gene="yejK"
                     /locus_tag="ACHUPM_000160"
     CDS             complement(48841..49848)
                     /gene="yejK"
                     /locus_tag="ACHUPM_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR0868065.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            50030..50257
                     /locus_tag="ACHUPM_000161"
     CDS             50030..50257
                     /locus_tag="ACHUPM_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR0868066.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            50277..52037
                     /gene="yejM"
                     /locus_tag="ACHUPM_000162"
     CDS             50277..52037
                     /gene="yejM"
                     /locus_tag="ACHUPM_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR0868067.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            52112..52188
                     /locus_tag="ACHUPM_000163"
     tRNA            52112..52188
                     /locus_tag="ACHUPM_000163"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52146..52148,aa:Pro,seq:ggg)
     gene            complement(52291..54291)
                     /gene="yejO"
                     /locus_tag="ACHUPM_000164"
                     /pseudo
     CDS             complement(52291..54291)
                     /gene="yejO"
                     /locus_tag="ACHUPM_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        13478 a        14180 c        13213 g        13420 t
ORIGIN      
        1 tgcggatata acattctgaa ccgcagctat ttgtgaatcc tttcacagtt taaatttccc
       61 cacacgctta gccttaatat cagcacgcaa tatttattta gcgttcgcct taattactca
      121 tagcattaag gaagatcaca tgccgcaaca aaattatctg gatgaactca ctccggcttt
      181 tacgccttta ctggcgatta aagaagcctc tcgctgttta ttatgtcatg acgctccctg
      241 tagtcaggct tgcccggcac agaccgatcc ggggaaattt attcgctcaa tctactttcg
      301 taattttaaa ggcgctgccg agacaattcg cgaaaataat gccctcggtg ccgtttgtgc
      361 cagagtgtgc ccgacggaga aattatgcca aagcggttgt acccgtgccg gtgttgatgc
      421 tcccattgat atcggccgct tacagcgttt tgttactgat tttgaacaac aaaccgggat
      481 ggaaatttat cagcccggta ctaaaacgct cggcaaagtc gcgattattg gcgcaggtcc
      541 tgccggattg caggccagtg tgacactgac aaaccagggt tatgacgtca cgatctatga
      601 gaaagaagca caacccggtg gctgattgcg taacggcatt ccgcaattcc ggttaccgca
      661 atcagtactg gatgcagaga tcgcccgtat tgaaaaaatg ggcgtgacca ttaagtgcaa
      721 caacgaagtc ggtaacacac tcacccttga gcagctgaaa gcagaaaacc gcgcggtact
      781 ggtcaccgtg gggttatcaa gcggttccgg gctaccgctg tttgagcata gtgacgttga
      841 gattgccgtc gacttcttgc aacgtgcacg acaggcgcaa ggcgatatca gcattccaca
      901 aagcgcatta attatcggcg gcggtgatgt cgcgatggac gtagccagca cgctgaaagt
      961 tctcggctgt caggcggtaa cttgcgtagc gcgtgaagag ttagatgagt ttccggcaag
     1021 cgaaaaagag tttaccagcg cccgggagct gggcgtttcg atcatcgatg gattcacgcc
     1081 agtagccgtg gaaggcaata aagtcacgtt taagcatgta cggttatcgg gcgaactgac
     1141 gatggcggca gataaaatta ttctcgccgt cggtcagcat gccagactag atgcctttgc
     1201 ggagttagag ccgcagcgta acaccatcaa aacacaaaat taccagaccc gcgacccgca
     1261 agtctttgct gctggcgata ttgttgaggg agacaaaacc gtggtctatg ccgtgaaaac
     1321 cgggaaagaa gccgccgagg cgattcatca ctatttagag ggagcttgct catgttaacg
     1381 aaagatcttt cgattacttt ttgcggcgtg aagtttccca acccgttctg cctctcttct
     1441 tcgccggtag gcaactgcta tgagatgtgt gccaaagcct acgacacagg ttggggcggt
     1501 gtggtgttta aaacgatcgg cttttttatc gccaacgaag tctcgccgcg ttttgatcat
     1561 ctggtgaaag aagataccgg ttttatcggc ttcaaaaata tggagcagat tgccgagcat
     1621 ccgctggagg agaatctggc cgccctgcgt cggctgaaag aagattaccc ggacaaagta
     1681 ttgatcgctt cgatcatggg ggaaaatgag cagcaatggg aggagctggc gcgcctggtg
     1741 caagaagctg gcgcggatat gatcgagtgt aacttctcct gtccgcaaat gacttctcat
     1801 gcgatgggta gcgatgtcgg gcaaagcccg gagctggtag aaaaatattg tcgggcagtg
     1861 aaacggggtt ccacgctgcc aatgctggcg aagatgacgc cgaatatcgg tgatatgtgc
     1921 gaagtggcgc tggcggcgaa gcgcggcggc gcagatggca ttgcggcgat taacaccgtt
     1981 aaatccatca ccaataccga tcttaatcag aaaatcggta tgccgatcgt taacggaaaa
     2041 tcaagtattt ccggatattc cggtaaagcg gtaaaaccga tcgccctgcg cttcattcag
     2101 caaatgcgca cccatccaga actgcgcgat ttcccaatca gcggtatcgg cggcattgaa
     2161 acctgggagg atgcggcgga gtttttattg ctcggcgcag caacgttaca ggtcaccacc
     2221 ggcattatgc agtacggcta tcgcatcgta gaagatatgg cgagcgggtt atcgcattat
     2281 ctcgccgatc agggatttga ttcgttgcag gagatggtag gtctggcgaa taacaatatt
     2341 gtcccggcgg aagatttaga ccgcagttat attgtctatc cccgtatcaa tcttgacaaa
     2401 tgtgttggct gtggacgctg ttatatttcc tgttacgacg gcggtcacca ggcgatggaa
     2461 tggagcgaga aaacccgcac accgcattgt aataccgaga aatgtgtggg ttgtctgctt
     2521 tgtggtcacg tctgcccggt gggttgtatt gagctcgggg aagtgaagtt taagaaaggc
     2581 gagaaagaac acccggtaac gttgtagaac ccgcgtcaca tccggcattt ggttcctgtt
     2641 gccggatgcg gcgtgaacgc cttatccggc ctacaaaacc acacagatca tgatgttatg
     2701 tgcgtttatg accgaatgcg gaccacattc acatcatttc ttacgcgcgt atttcagtga
     2761 atccagcgct acggcgaaga taataatcgc ccctttgatg atgtactgcc agtatgggtt
     2821 tacgccgata tacgtcaggc catagttgat gacggtaaaa ataatcaccc cggtcaccac
     2881 gccaatcacc gtccccacac cgccgctgaa cgatacaccg cctaccacgc acgccgcgat
     2941 agcatccagc tcatacataa agccgaggtt gttggtggca gagccgatac gtccggcttc
     3001 taacatcccg ccaaaggcat agaacacgcc agacaacgcg tagatcatca gcaggttcag
     3061 gccgacgttg acaccagata cttttgccgc ttccgggtta ccgccaatgg caaaaatgtt
     3121 cttaccgaag cgggttttgt tccacaacac ccagacgaac gccaccgcaa tcaacgcgta
     3181 gaaggtgatg taagagagac ggaaactccc cagcgcgaca aagccctgag caaaggtaga
     3241 gaagccactg tcaaaaccag aaattggcga cgccccgaca aagtcgtaat agagcgagtt
     3301 gatgccatag acgatgatca tcgtgccgag cgtggtaatg aacggcgtca cgttgagata
     3361 agcgataatc agaccgttga tcaaaccgat caccgcacca atggcacaga caatcagaat
     3421 aaccagcgca atcggcatcg tcgccatttc cgggaacact ttgttggcgt tatccatgga
     3481 ctgcaataat gtcgcagcca ccactgccgc cagccctacc tgacgaccag cagaaagatc
     3541 ggtcccctgg gtgacaatta accctgccac accgagcgcg ataataatac gcaccgatga
     3601 ctgggtgaga atattactta agttcaacag acttaaaaat gttgggtcct ggaaaataat
     3661 aatcgccagc aaaactaaaa gaacgacgta aataccgccc tctttcaggt aagtaagaaa
     3721 acttttctta tttaacgcac tcatgggaag cccctaatct taaaggtgca aagacgcaag
     3781 acgcagaatt tcgttttgcg ttgttgtttt tgtatcgaca attccggaaa cgagaccatt
     3841 gctcatgacc agaatacggt ctgttatccc taacaactca ggcatttcag aggagataat
     3901 aataatcccc ttgcctttct tcgccagttc ggcaattaac tgataaattt caaacttcgc
     3961 cccgacatca ataccgcgcg tcggttcatc gagcattaat atttctggtt gcgttagtag
     4021 ccagcgacca ataatcacct tttgctgatt accaccggag agcgaaccaa tttgcgtccg
     4081 atgacccggc gtttttaccc gcatcgaatc aatcacccac tgggtatcgc ttttcatccg
     4141 cgagttatcc agtaaaccaa ctttattttt gtagttgcga atattggaaa ttaacgagtt
     4201 aaaaccaata tccagatagg cataaattcc cgttgagcgg cgctcctcag ttaccagtgc
     4261 aaatccgtgg tttatggctt cgttggcatt atggttattg atctgtttgc cgtgcaacgt
     4321 aatggtgcca gccgatttct cgcgaatacc aaataacgtc tcaacaatat cggtacgttt
     4381 cgcccccacc agcccggcaa taccgaggat ctccccttta tgcagatcaa acgagacatc
     4441 gcgaatcgac ggctggcgca gtgacgttag gttacgcacc tcgaggatga cttcgcccgg
     4501 cttgttttct ttgtcaggaa aacgctgatt aagagaacgc ccaaccatca tggcgatgat
     4561 cttgtccatc gtcagtcctg ccagcggttc ggtggcgatc cactgaccgt cgcgcaatac
     4621 ggtaacttca tcacataact ggaagatttc ttccatttta tgcgagatat aaacaatacc
     4681 gcagccgcgc tcttttaatt tacgaataat agtgaacaga tgattgacct ctttttcggt
     4741 taacgaagaa gtcggttcat ccataatcac aattttcgcg ttataggaaa acgctttggc
     4801 gatttcgatc atctgcattt gcgaaacgga taatgtgccg acgcgcgcac gcggatcgat
     4861 atcaatatcc agttcatcaa aaattgcttt ggtttcgcgg tacattttgt cctgatcgac
     4921 aaacatgcct ttggtgggat atcgccccag ccacatgtta tccatcaccg aacgttgtaa
     4981 taccaggttt aactcctggt gtaccatcga aataccattt tccagtgcct ctttggcgga
     5041 atggaaatcg atctctttac cctggaataa aatggtgccg gagtcttttt gataaatacc
     5101 aaacaggcat tttaataatg tcgattttcc tgcaccgttt tcccccatta atgcatggat
     5161 agaatgtggc cggactttta aattaacgtt atcaagtgcc ttaacaccag gaaaggactt
     5221 gttgataccg ctcatttcca acaagtattc cccggaagac ggagtcgttg agctgaccat
     5281 ataattttac cttgttggcc atacaataag ggcgcagtaa acgactgcgc ccaatcagtc
     5341 ttatttcttg ctgaattcag ccaggttgtc tttatctacg ccaacataag gtacgcggac
     5401 cactttgttg tcgattttcc agttggtgcc atcagccgca cctttgccat cggccaggtt
     5461 tttcgccaga tcaaaggtcg ctttcgcctg gttgttagca tcgttcagta ctgtgcccgc
     5521 cagtgcaccg gatttcacca gcgccagcgc ttctggcagc gcatcgacgc caaacaccgg
     5581 aatgctggac ttgttgtgtg ctttcaatgc ttctaccgcg cccattgcca tcgcatcgtt
     5641 gttggcgata accacttcga ttttgttggc gttcgggcca gacagccagg cgtccatctt
     5701 atctttcgcc tgagcggtgt cccacattgc ggtatctaac tgtaactgtt cagttttgat
     5761 gcctttatcg ttcaattctt taatcacgta agtggtacgt gcttctgcat ccggatggcc
     5821 cggttcacct ttcagcagta cgaactgaat ctgaccgtct ttgttcagat cccaaccctg
     5881 attcgccgcc cagtgtttag caatcaaatc gccctgaata atgccggact ctttggagtc
     5941 agtgccaacg tagtaggctt tgtcgtagct atccagcgcc ttacgagacg gttctttgtt
     6001 gaagaaaacc accggcacgt tttgcccacg cgctttctca atcaccgtac ccgcagctgc
     6061 cgggtcaacc aggttgattg ccagtgcctt cacccctttc gccagcaata cgtcgatctg
     6121 atcgttctgc ttggactggt cattctgaga atcattcatc agcagctgaa catctggcgc
     6181 ggctttcgca tcttgctcaa tagccttgcg cactacagac ataaagttat cgtcgtactt
     6241 atagattgtt acaccaatgc gagtatcagc agcgtgtgca gcggcaccga ataacatgct
     6301 ggccatcaca gcagacaggg ttaacacctt cttattcatg gtatctccgg tttttcttat
     6361 gcagggtagt gcttgagata aatgctcggc ggggcagtag agttaatgaa gtgttactga
     6421 acgccgaagc tcactttttt aaattcgttc ttccatgccc ggtaacgctc cagaaaacgg
     6481 ctttaattgt tgttatgacg ctattacctc ggtgaaagtg attattcact gttacatacg
     6541 ggttacaacg ttaaaacggt gcaatcatag ctatcacatt gttaagatac tgtgaaatca
     6601 ctcacagatt gaaagcggtt acatcgcctg atttgttgag ttagtgatcg ccgccgcatt
     6661 ctggcgcgtt gcgacagaat ggcgacgcac taacgtcggc ataaaacagt ggctggcacg
     6721 aggatcaata ttgcctgctg ccccctgcaa ggccagttcg gtggctaatt tagccattga
     6781 agcaatggga taacgcacgg tcgttaattg cgggtcggtg taacgggcga tgggaatatc
     6841 atcgaaaccg atgattgaga gatgtaacgg aatcgcaatg ccattatctt ttaatgctgt
     6901 cagtgcgcca gcggccatat tgtcgttata agcaaatata gcggtaagtt gtagattgcg
     6961 ccccagcagt tcaaccatcg ccgcctcacc gcccggcatg tccggcgtac cagtgccaat
     7021 ccagctttcc ggcggaataa tatcctgctc tttcaacgca ctcatccagc ctgctttacg
     7081 catggcgtca tcttcaatgc cgtgactgga agaaagataa ccaatacgtt gatgaccgtt
     7141 attcagcaac atgcgcgtcg ccattcgggc accgctgaga ttatccaggc aaacgcaacg
     7201 atgggcgtac cccggcacaa cgcggttgat taacaccata ccgggaatgt tatccataaa
     7261 ttgcgccagt tcatcgtcac tcaatgcttt tgagtgaaca atcaacgcat tacaacgctg
     7321 gcgaattaac acctcaatgg cgtgacgctc tttttccgct tcatgatagc tattgccgat
     7381 tagcacgtat ttctgatgct gctgagcgac tagatccacc gcttttacca gcgcgccgaa
     7441 aaacgcatca gaaacgtcca tcaccaccac gccaatggtg tcgctaacct gagttgccag
     7501 cgcctgggca ttggcgtttg gccgataatc cagctcactc acggctttca ttactgcttc
     7561 acgcgtgtcg gcactgacca gcgtgctgtt attgagcacc cgggaaaccg ttgccacaga
     7621 gacgccagcc taacgcgcta catcacgaat ggtgatcata ttcactacct gttatgagat
     7681 tgcagtaaat gactgcttgc tggcggctat tttgtcagca ctgaatacag gacttcgtga
     7741 atcgagtcac agcaatggaa acggttacag ccgttttgtt aacgaatgtg atccagatcg
     7801 ttatcttgat gttttagata ttgccgttcc ggcagcacgc aaagttaact gacgccagag
     7861 ccattccacc ggcccctggc ggaagtaacg cagccagata acagagaaga ggatattcgc
     7921 cagccatacc ggaataacaa acgccagcag ctccaggcgg tcaaaatgca taaacaaacc
     7981 gaggtggtaa aaaagcatgg tacaaatcag cgtttgcaat agatagttgg tcagcgccat
     8041 ccgtccgacg caggcgatcg caagcaccag cttaaagcgg ctcaattgcg gccagaagcc
     8101 ataaaacagc gacgcatagc caatcgcctg aaacggcgca ctcagttccc gcggcatttg
     8161 aagtaagaag gcacaccagc gatacgccca gtccagctgc cattgcaggg cgatggcagg
     8221 aaggttaatg gtcaccccaa tcgccaccag cacaaaacca gtacgacgat agtgacgtaa
     8281 gctgaactgc cctttcagcc agccgctgcg catcagcgcc gcaccaatga gcatcatccc
     8341 tgccagttgc cagccatact gtgcgcccag tgccagtaaa ctgttgccaa caccatcggc
     8401 acgattactg atcgcttcaa cgccgccgtg aagcttccag tatttttcat acaaaatagc
     8461 cgatgcatcc ggcgtccagg tgcggctggt ctggctgtcg gaaatcagcc ccaataacag
     8521 caaaacgcca agccccacca gataaagcat gacgcctgta ttaaacaggc tttttaccga
     8581 tggcgcatcg cgcaccagcc gccagcagat taagcccacc agcccgtaag ccagcagaat
     8641 atcgccgtcc cagaacaata aaccgtgaat aaaacccagc aagactaaca gcgttaaccg
     8701 cgactggatc cagcgtctgc cacggggcag caacatttgc aggcccgcac caaacagcag
     8761 cgcaaaaagc gtgaggaatt tcacctggcc gatgagatcg agaaatgccc aggtccatgc
     8821 atcctgcggc gtaatagcgc cgtaccaggc gggattgaga taagccgcct ttggtagccc
     8881 aaaggcgctg atgtttaata gcaggatccc cagaatggcg acgccgcgaa caaaatcgag
     8941 cgttacgttg cgctccatgg ttcctgcctt ttaatcagtt gtgatgacgc acagcgcgca
     9001 gaaactcgtg gcgcgtattc tgactggatt tgaacaatcc accaagagag gtcgttgttg
     9061 tggcactggt tgcatcgcgg atgccacgcg ctttcacgca gtaatgcacc gcatcaatgg
     9121 agacagccac gttattggtg cccagcagcg tttgtagcgc aataagaatt tgctgcgtca
     9181 gacgttcctg cacctgcgga cgctgggcaa agaactgcac gatacggtta atttttgaca
     9241 gaccgatcac cgaatctttc gggatataag ccaccgtcgc tttgccatcg atggtaacaa
     9301 aatggtgttc acaggtactg gtcagagtga tatcgcgcac ggtgaccatt tcatcgacct
     9361 tcattttgtt ttcaatgagg gtgattttcg ggaagttggc gtaatccaga ccggagaaaa
     9421 tttcatcgac atacatttta gcgatgcgat gcggcgtttc catcaaactg tcatcagcca
     9481 ggtcgagatt cagcagctgc atgatttcgg tcatatgacc agcaataagg cttttgcgcg
     9541 tttcgttatc catttcatgc acgggcgggc gcagcggtgt ttccagtcct cgcgcaacta
     9601 acgcttcatg aaccagggcc gcttctttac tgagtgatgg catttatgat ttctcctgca
     9661 ggtgtgacgc ctccgccctg cgtgggggca aagttattaa gctgatttac agcctgatta
     9721 ttgtgcgtga ggcggcgcac ataatccagt attcacagcg ataattattg taattgccgc
     9781 tgactttcat cagcagatgt taaaacatcg ttatgcaaat acggaagtga aagttactca
     9841 cagcacattg aataaacggt atgatgaaga aattgcaaac aacacaacaa ggagccacgc
     9901 atggaaatgc tcgaagagca ccgctgtttt gaaggctggc agcaacgctg gcgacacgac
     9961 tccagtacct taaactgccc gatgacgttc agtatctttc tcccaccccc tcgtgatcac
    10021 accccgccac cagtgctgta ctggctttcc ggattaacct gcaatgacga gaacttcacc
    10081 accaaggcgg gtgcccagcg cgtggcggca gaactgggga ttgtactggt gatgccagac
    10141 accagcccgc gcggcgaaaa ggttgccaac gacgatggct acgatttagg ccagggcgca
    10201 ggcttttatc ttaatgccac gcaaccgccg tgggcgacgc attaccggat gtatgattat
    10261 ctgcgcgatg aattaccggc gctggttcag tcgcaattta atgtcagcga ccgctgcgcc
    10321 attagcggtc actcaatggg tggtcacggt gcgctgatta tggcgctgaa aaatccgggt
    10381 aaatacacca gcgtttcggc ctttgcgcca attgtgaatc cgtgcagcgt cccgtgggga
    10441 atcaaagcgt ttagcaccta tttaggtgag gacaaaaatg catggctgga atgggatagt
    10501 tgcgcgctga tgtatgccag taacgcgcag gatgcgatcc cgacgcttat cgatcagggc
    10561 gataatgatc agtttcttgc cgaccagttg caacctgcgg tattggcaga agccgcgcgc
    10621 cagaaagcgt ggccgatgac gctgcgtatt cagccgggat atgatcacag ttactacttc
    10681 atcgcctctt ttatagagga tcacctgcgc ttccatgcgc agtatttact gaagtgaaag
    10741 tccgcccggt tcgccgggca tcttctcatc agaagcgata atccactgcc ataaagtaac
    10801 gacgtccgtc ttcgttatag ctgtagtcgt cacgactgag atccttgtcg ccaaggttca
    10861 gcacgcctgc gcgcagtttg acgtctttag tcacctgcca ggccgcgccg gtattccaga
    10921 tggtataacc gcccggtgtt ttagccgtcg cgctgtcggc gcgtttctgc ccggtatagt
    10981 gaccagaaac atagaatgac cagtcttcca gcgccagcgg tttccagtcc agcgtaccgt
    11041 tagcagtatg gaacggcaga tcggatagcg gtttgttttc gccgttgctg acatcacgac
    11101 catcgttgta ggtgtagttg atcgacagtt tccattcatc gttgaacgga attttcagtt
    11161 cggtttccac gccctgaata cgagctttgt taacgttgta gtagctaaat accggtatgc
    11221 gccgtccgtt agcgcccgtc tcaaaaccaa caaagttttg gtagcccggt gcagcgttga
    11281 cgtcagacgt acggctgatg ctgatacgat ctttcacatc gttacggaaa acggtaacgc
    11341 tggattcaac cccttccagc cagccttctt cacccatgta gtaaagcccc agctcccaac
    11401 tttcgctggt ttctggtttc agatccgggc tacccacaat cttacatgcg ccacggcagg
    11461 aattgctcgt ccagtcaggg ctaagttgca acagagaagg tgctttaaat gccgtcgccc
    11521 agcccccttt caccgttacg gtgtcggtgg cgttataaac caggtaggca cgcggactcc
    11581 agtgttcacc gtaggtttcg tgatcgtcca tacgcacgcc ggtcgtcagc gccagcggct
    11641 cgaagatccg ccattcatct tccacaaaca gcgcgtactg gctggcagac gttttggagc
    11701 tggttccccc ggtcaggttc accgcatcgc taagtttgtc gtgacgccat tcaccgccaa
    11761 ccgtgagaaa ctgattaatc gccgtcagcg gcaacgtgta tttgccgtcg accgtattgc
    11821 tttcggaagt tatcgggctg ctgttgccag ggtttttgtt ctcgactttc tcaccgtagt
    11881 atttcagttc gctggtgccg taatcccaac gcccattatg gctgacggag tagttctggc
    11941 gttccaggcg gtttttgtcc agcgagtcgg aatcacgatc ctgacggtcg aaaccgtatc
    12001 cggcagtaaa atcgtgattt tgattcggtg tccaggcaaa ttcgacattg ccgtcgcggc
    12061 tggagaatcc ttcaatacgc ggcgtttctc cggtatcggt ggtcgttgag ttttgcgggt
    12121 catccttttc acgttttgcc aggctgccgt aagctttcat tcccagcaca ccatcaatta
    12181 atggtccact ggtaaagaac tgaccgttat aggtatcacc gcgatcgcga tgttcctgaa
    12241 tggtggtatc gacggtaacg gtacccgacc atttctgacc gatttttttg gtgatgatat
    12301 tcactacacc gccgagcgca tcggaaccgt acagcgacga catcgggcca cggaccactt
    12361 caatacgttc gatggaatcg accgggatcc agttcagatc gaaatcattg tggcggaaga
    12421 cggcattgcg ggagttcacg cgtttaccgt cgacgagaat cagggtatag ctgctgtcca
    12481 gaccacgaat actaacgccc ttacggttat ccccttcgtt cgtcagttgt acgccaggca
    12541 cttctttgag gacatccttc agattctgta ccggttttcg ctgcagatct tcctgggtaa
    12601 tgacgctgat actggcgggt gcatctttaa ggttttgttc aacggaagat gcagtgacaa
    12661 ccatcgtttc gccatcgtca tcgaccgcta acacaggcca tacacaagaa atagcggaca
    12721 aacacagccc gacccgtacg aaagggttca acctaaacat tccatatctc catgaggtaa
    12781 ctacgaaaat aaaatgggta tcgctcacat cttctttacg tccccttgcg tacggcagca
    12841 tcgcggtgga cttatttgta tgcaggtgat catcccagaa agccggacca gcctctgatt
    12901 tgtcagcctt ttttgattgt agcgtaacga taatgcaaac gataacaatt atcaatccaa
    12961 atgttaaatt ttatatccgc atgatttgtc agggaaatgg aattgaaaaa gccctctcgg
    13021 ttgagagggc ttagcaagga agggaggaaa cttatttctt atcgttctgc gggaacttca
    13081 tttcgctgta gcgtacgaag tgagttcctt taatcagctt gtagccgaac caaataatca
    13141 ggaacagcgg gataccaata tacgttgccg ctacgccgcc ccagtcaatg gtatctttca
    13201 ggaacgcttc gtagttctgg cccaaagtga taatcagaca cagaatgaat gcgaagatcg
    13261 gccccagtgg gaagaaacct gaacggtacg gcagatcgtt aatgtcgtgt ccctgcaata
    13321 cgtaaccgcg acggaagcga tagtggctaa tggcaatccc cagccaggcg ataaaacccg
    13381 tcatcccgga ggtgttcagc agccacaggt ataccgtctg gttgccaaac atggaggtca
    13441 ggaagcacaa accggcaatc accgtcgtcg catacagcgc attacgcggc acgccaccac
    13501 gcgacaattt agcgaaaatg cgcggcgctt taccgtcaca cgccagggtg tacagcatac
    13561 gagtagacgc atacataccg gagttacctg ctgacagcac cgccgtcaga ataactgcgt
    13621 tcatcaccgc cgccgcagag agcagacccg cgtgctggaa caccaaggtg aacggactga
    13681 cgctgatgtc tttaacatcg ttacgcagca ggctcggatc ggtatacgga ataatcaggc
    13741 tgataatcag gatcgcgaac acatagaaca acaggattcg ccagaatacc tgacgtaccg
    13801 cgcgtggaat gtttttcgcc ggatcttcgg actcgcctgc agcaataccg atcagctcgg
    13861 ttccctggaa agagaagccg acaatcatag ctacgccgat catcgccgca aaaccaccag
    13921 caaacggcgc ttcgccgatt gtccagttgc tccagcccgc aggctgcgcg cctttgaaga
    13981 taccgataat catcagcacg ccaacgatga taaagacaat aactgtcgtg actttgatca
    14041 gtgaaaacca gtattccgct tcaccaaagc cacgaactga gatgtagttc agcaggaaga
    14101 taacgccgag gaacaacgca ctccagatcc agcccggtgt atccgggaac caccagctca
    14161 tgaccagctg tgctgcaacc aagtcaacgg caatagtcac cgcccagttg taccagtagt
    14221 tccagcccag cgcgaagcca aagccttctt caacatagtt ctgaccgtaa gtggcaaacg
    14281 aaccggaaac cggcatatat gcagccaatt caccgagact ggtcatcagg aagtaaacca
    14341 tcaggccaat cagcatatac gagagcaatg ccccgcccgg acctgcctga gaaatcgttg
    14401 cgccagaggc aacaaaaaga cctgtaccga tggaaccgcc aatggcaatc atcgtcaggt
    14461 gacgcgcctt taattcacgg cgtaagcccg gcgcttctgt agttttagtt tcggaaccca
    14521 tacaaaaatg ctatccatct taaaaatgag gcgcgattgt agcagacgat tggcaatcct
    14581 tccggcagaa atacacggtt ataagacacc ttcatgatcg cccagggatt ataagtaaag
    14641 cagccaatct cttttctggc gaatgcatgc tgagggcgct gcgtcacgaa atcgacacag
    14701 caccagcatg ttcttgtaca gcaccttaac gcggtacatt cgcgggatcg caatagtcca
    14761 gaaagcgccg tagcgcgttg gaaaggtgtt tttgccgatg atgtatacgc cacaacgtac
    14821 gcatcaggcg cgggagaggg accgcaactt cacttaatgt gcctgcctgc aattgatctt
    14881 caatcacacg tcgcgacagg caactaattc ccaacccatg acgcaccgca tgtttgattg
    14941 cctcggagtt acctaattcc atcgccatct caaacttcgg taaatgtgac agcaacagat
    15001 aatcgacaat ctcccgcgtg ccggaaccgc gttcacgcag gatccacggc gcagcggcca
    15061 gctgttctaa ggtgaccgga ccacgagcca acggcgaagt cggcgcggcg aaaaccacca
    15121 gctcgtcttc cagccacggt tcagaaatga tttcagtgct gtggcacggt ccttcaataa
    15181 agccaatatc aacgcggaaa tccagcaccg cttgcatcac gtcctggctg ttcccaacgc
    15241 taagttcaat cggcaactgc ggataatcat ggcgataacg ggcgataact gcaggcagaa
    15301 tgtagttacc gatggtacta ctggcataga tacgaatcgc gccgttgtct tcgcgaaaca
    15361 gttgttcgat ttcaaccgcc tgttcaagca atgccaacgc acgcggatag agcagccgcc
    15421 cgtgttcatt aacaaccagt cttttcccca cgcgatcaaa cagttgcacg ccaagctgcc
    15481 cttccaggtc ggtcaaggct gcgctcactg ctgattgcga caacgccagc atcaccgacg
    15541 cctgggtggt tgatccactt ttcaatactt ctgcaaaaac ttccaactgc cggagggtga
    15601 tgtgcatagt cgcttaccac ttataaagat taattataaa tatataatca attttatttt
    15661 taaaccagtt agtcgtaacc ttatgcccgg ttaaaggaga gggttatgac gaatatcacc
    15721 ttacagaaac aacatcgtac actgtggcat tttattccgg ggttagccct gagtgcagtt
    15781 atcaccgggg tcgccctgtg gggtggttcc attcccgcgg ttgcgggtgc cgggtttagt
    15841 gccctcaccc tcgcaatctt gttggggatg gttttaggca acaccatcta tccgcacatc
    15901 tggaaaagct gtgacggtgg tgtgctgttt gccaagcaat atttattacg tctgggtatc
    15961 attctttatg gcttccgtct gacgttctcg caaattgccg atgtcggtat cagtgggatc
    16021 atcattgacg tgttgacgct gtccagtacc ttcctgctgg cttgcttcct ggggcagaaa
    16081 gtgtttggtc tggataagca caccagctgg ttgatcggtg ccggtagcag tatctgtggt
    16141 gctgccgcgg tactggcgac tgagtcggta gtgaaagcgg aagccagtaa agtaaccgtg
    16201 gctgttgcaa ccgttgttat cttcgggacc gtcgcgattt tcctctaccc ggcgatatat
    16261 ccgctgatgt ctcaatggtt tagtccggaa actttcggta tctacattgg ttctactgtg
    16321 cacgaagtgg cgcaggtggt ggcggcaggt catgccatca gcccggatgc ggaaaacgca
    16381 gcagttattt ccaaaatgct gcgcgtgatg atgctggctc cgttcctcat cctgctggcg
    16441 gcgcgtgtta aacagctgtc tggggcgaac agcggcgaga aaagcaaaat cactattccg
    16501 tggtttgcca tcttgttcat cgtcgttgcc atctttaact cgttccacct gttaccgcag
    16561 agcgtggtga acatgctggt aacgctggat accttcctgc tggcaatggc gatggcggcg
    16621 ctgggtctga ccactcacgt cagcgcgctg aaaaaagctg gggcgaaacc tctgctgatg
    16681 gcactggtac tgtttgcctg gctgattgtt ggtggtggtg cgattaacta tgtgattcaa
    16741 agcgtcatcg cataaaccac tacatcttgc tcctgttaac ccgctatcat taccgttttc
    16801 ctccagcggg tttaacagga gtcctcgcat gaaatacatt ggagcgcacg ttagtgctgc
    16861 tggcggtctg gcaaatgccg caattcgcgc cgtcgaaatc gacgcaaccg cgtttgcctt
    16921 gttcaccaaa aaccaacgtc agtggcgtgc cgcaccgctc acgacgcaaa ccatcgatga
    16981 attcaaagcc gcctgtgaaa aatatcacta cacatcggcg caaattcttc cccacgacag
    17041 ttatctgatt aacctcggac atccggtcac tgaagctctg gaaaaatcgc gcgatgcctt
    17101 tatagatgaa atgcagcgtt gcgaacaact ggggctttct ttgctcaact tccaccctgg
    17161 cagccatctg atgcagattt cagaagagga ttgccttgcg cgtattgccg aatccatcaa
    17221 cattgcgctg gataaaactc aaggtgtaac agcggtgatt gaaaacaccg ccggtcaggg
    17281 cagtaactta gggtttaaat tcgaacatct cgcggcgatt atcgacggcg tggaagataa
    17341 attccgcgtc ggcgtctgca ttgatacctg ccatgctttc gctgccgggt atgatttgcg
    17401 tactccagcc gaatgcgaaa aaacgttcgc tgattttgcc cgtattgtcg gctttaagta
    17461 tctgcgcggg atgcacctta acgatgcgaa aagtaccttt ggcagccgcg ttgaccgcca
    17521 tcatagcctc ggtgaaggca atatcggtca tgatgcgttc cgctggatca tgcaagacga
    17581 ccgtttcgac ggcattccgc tgatccttga aaccatcaac ccggatatct gggcagaaga
    17641 gatcgcctgg ctgaaagcgc aacaaactga aaaagcggtg gcctgatcga tgaacgacag
    17701 ggaaaagcag attcttaaaa ttttacggcg taacccgctg attcaacaga atgaaatcgc
    17761 cgatattctg caaatcagcc gctcacgtgt tgccgctcat attatggatt tgatgcgcaa
    17821 gggtttgatt aaaggcaaag gatatatcct taccgagcag gattactgtg tggttgtggg
    17881 tgcaatcaat atggatattc gcggaatggc ggatatccgt tacccgcaag cagcctctca
    17941 tccaggttct gttcactgtt ccgcaggcgg cgtcggacgt aatatcgcgc ataacctggc
    18001 tctgcttggt cgggatgtgc atttaatatc agctattggc aacgattttt acggcgagac
    18061 attactggaa gaaacgcgcc gcgcgggcgt gaacgtttca aactgcattc gtctgcatgg
    18121 acacagcact gcaacttatc tcgctatcgc taataagcag gaagaaacta tcctggcaat
    18181 caatgacaca catattctgc aacagctaac gcctcaactg ctcaatactt cacgggactt
    18241 gattcgccat gctggtgtgg tgctggctga ttgtaatctc acacctgaag ccctggagtg
    18301 ggtctttacc attgctgatg aaattccgat gtttgtcgat accgtttccg aatttaaagc
    18361 gaacaaagta aaaaactggt acagccggat ccacactctg aaaccaacac aaaatgaact
    18421 ggaaattttg tgggggcaac cgattaaaga cgacaacgat cgcatcagag ctgtaaattc
    18481 gctgcatcaa caaggtgtta aacgcatttt tgtttatctg aaagatgaat ctgtattttg
    18541 cagcgataag gatggagaac aatttttgct gaccgcgcca gcgcatacga cagtagacag
    18601 ttttggtgct gacgatggtt ttatggcggg cctggtatat agctttcttg aaggatacag
    18661 tttccgcgac agcgcccgtt ttgcggtagc ctgcgcggca atttcgcgcg ccagcggcag
    18721 cttaaacaac cctaccctgt ctgccgataa cgcgctttca ttagtgccaa tggtgtaaca
    18781 atgttgccgg attcgacgct tgaccgcatc ttatccggct cataaattgc gcactacgct
    18841 aaaccaataa agaatcctgc aatagtagca ctcatcaggt tagaaagtgt cgccgccgcc
    18901 agcgcgcgta aaccaagttg ggcgatttcc ggcgcacggt gtggcgcaac cgcagaaaac
    18961 gcccccacca ccaccccgat agaaccaaag ttagcgaaac cgcacaacgc gaaagaaata
    19021 atcgccacgg ttttagcatc cagagtgcca cccgtttgca gatatggcga gaaattgaga
    19081 taagcgacaa attcattgat cgccagcttc tgcccaatca aactcccggc aagatttgca
    19141 tcactccagt caacccccat cacccacgcc aatggggcca acaggtaacc taaaatcgac
    19201 tccagcgaag catgttcaaa accaaaccag ccgccaacac cgccgataat accgttaatc
    19261 aacgcaatta ttgcaacaaa tgccataaca actgtcgcta caccggcggc gattttcagc
    19321 ccggtcattg cccctgtcgc agcggcttca ataatgcttt ttggcggtgt ttcggtgaaa
    19381 gagaggttat taaaagaaac ctgcgaagat tccgtagccg ggcttaacag gcgggcaaac
    19441 aagatcccgc cagggatcgc cattaacgat gccgccagca aatattccac aggtacgccc
    19501 agtgcggcgt aaccaatcat tgtcgaacca gcaatcgagg ccatgccact acaaatcgct
    19561 gtaaataatt cattgcgatt cagacgatcg ataaagggtt tgacgatcgc cggaatttcg
    19621 ttttgcccga ggaaaatggt ggtgaccgcg acgaatgact cgatcttgct gatatttaaa
    19681 gctttctgga agataccgcc gagaatgcga attaagatcc ccatcacacc gatgtagtag
    19741 agaatactca ccagtgcagt gacgaagata attgccggta ataccctgaa accaaagata
    19801 aatcctgcgc catcaaataa ggtatccatt ttcggtccga ccagagaacc gaagataaat
    19861 gcgctacctg cgtcgctgta cgccatcact ttgtggacgc caaaagcgac tttttctgcg
    19921 acccaacgcc ctggcggtaa ccaaagcata atcccgccaa tcacgacctg taacactaac
    19981 gccgcgccaa cggtacgcag gctgatcttc ttcttgttta ctgacagtaa aaacgcaatt
    20041 gccagcagta ccaccattcc cagaacactt ctcatgacat ccatagtgat tatctcttca
    20101 tgtcattttg ggcgacggct ttctgcccat cagaaatagc cgggcctgca tcttgcgata
    20161 ctgtcccggc ttgttatcaa cacttaatgg gtttttatgt agccgcgtac gcactcttcg
    20221 actaatcccc agaaccagtc ggtatcaata gtgatgccga ctttagtatt ggcgggcttg
    20281 ccaagaacgc ccagctcgtc gcagacggta cgcccatagc aagggccact gttgacgtcc
    20341 acttcgacgt acatctcctg ggttttaatg ccatcagggt taatcagata accgatgcag
    20401 gtggcgtcgt gcaccgggcc gccagccagg ccgtagtttt cgaactgcgt tttgagagtg
    20461 aagttcatga tgtcgctgaa catctctccg gcagggccgc ctgccctttc cattcgtgca
    20521 attacgtccg gggtgcaaac ggtctggttg gtaagatcga ggcccatcat cactaatgga
    20581 acgccggagg tgaacaccac gcgtgcggct tccgggtcgg caaagatatt gaactcggca
    20641 gatggggtga agttgcctgt accgtaagcg ccgcccatta gcacaatttc acggattttg
    20701 ggcaggattg cgggttgcat acgcattgcc accgcgatat ttgaaagcgg accaaccggc
    20761 accagagtga tatcgccatc gcttgccata agggtatcaa tgatgtattt caccgcgtgg
    20821 gtactttctg cctggcgggt cagcggctcg aataccgggc catccagtcc ggtttcaccg
    20881 tgaatattat cggcaacgat ttgttgacgc ataatgggtt gcggcatccc cgcataaacc
    20941 ggaacattaa tctccagttt ctggcaaaca ttcaggccat taattaatgt tttatcaagc
    21001 gtctgattac ccgcaacaat ggtgatacct aataaatcaa tcgccggatg tttcgccgcc
    21061 atcattatag caatagcatc atcatgaccc ggatcacaat ccagaataat ttttctcttt
    21121 tccattgttt atttcctctg tttccagttg cgttattttt tctacagcaa cgaaatttcg
    21181 atcgggccga tgattgaatc ttaacaacag cgtacgaatg ctaaatatga gaaatctcat
    21241 agcggataaa catcgtgaaa gaaatccaca ataatgatct taagcagcaa ttgatgagtg
    21301 aatccgcgtt taaggattgc tttttaacgg atgtttcagc cgatacgcgg ctgtttcatt
    21361 ttttagcgcg tgactacatt gtgcaggaag ggcaacagcc gtcctggctg ttttacctga
    21421 cgcgaggccg cgccaggctt tacgccacgc tagctaatgg tcgcgtgtcg ctgctcgatt
    21481 tctttgccgc cccctgtttt attggcgaga ttgagttaat cgacaaagac catgaaccgc
    21541 gtgcggtgca ggctattgaa gagtgttggt gccttgcgct ccctatgaaa cattaccgcc
    21601 cgctgttatt aaacgacacg ctatttttac gaaaactctg cgtcacctta agtcataaaa
    21661 attatcgtaa tattgtttct ttaactcaga atcaatcatt tccgttagtg aatcgcctgg
    21721 cagcatttat attactctcg caggaaggtg atctttatca cgaaaagcat acgcaagcgg
    21781 cagagtattt aggcgtttct tatcgacatc ttttatatgt tctcgcgcaa ttcattcacg
    21841 acggtttatt aattaaaagc aagaaagggt atctaattaa aaacagaaag cagttgtcag
    21901 gactggcgct ggagatggac ccggagaata aattctccgg gatgatgcag taaaaattat
    21961 tggcaatagc acgattgccg gatgcaacgc ttaataaggc gtacatccgg caatgaaact
    22021 caagctaaac caataaagaa cccggcaatg gtcgcgctca tcaggttgga aagcgtcgcc
    22081 gccgccagcg cccgtaaacc aagctgggcg atttccggcg cgcgttttgg cgaaatagcc
    22141 gaaaacgctc caacgacaac accgatagaa ccaaagtttg caaacccaca gagggcgaac
    22201 gagataatgg caatggtctt gacatccagc gtgccgctcg tttgcagata gggcgagaaa
    22261 ttgagataag caacaaactc attaatcgcc agtttctgcc caatcaaact tccggcaaga
    22321 tttgcatcac tccagtccac ccccataatc catgccaacg gggccagcac atagccgaaa
    22381 atgctttcca gagagacatt ggcaaaacca aaccagccgc caactccacc aataatgccg
    22441 ttgatcagcg caatgatcgc tacaaatgcc attaccaccg tcgccacacc ggcggcgatt
    22501 ttcagcccgg tcattgcacc gttcgccgca gcttcaataa tgctttttgg cggtgtttcg
    22561 ctaaacgaca gattttcaaa cgtaacttgc gaaggttccg tcgccggact aagaatacgt
    22621 gcaaacagga taccgccagg aatagccatc agcgaagcgg ctaacaaata gtcaatcggc
    22681 acccccattc cggcatagcc aatcatcatc gaacccgcaa tagacgccat gccgctacaa
    22741 atagccgtaa atagctcgtt gcgattcatg cgatcaataa aaggtttaac gatcgccggg
    22801 atctcatttt gcccgaggaa aatagtggtg actgcgacaa aagattcgat tttgctgatg
    22861 ttgagggctt tctgaaaaat actgccaaga atacgaatca gcagccccat tacgccaatg
    22921 tagtacagca agctgataag cgcggtaacg aaaataatcg ccgggagtac gcgaaaggcg
    22981 aagataaagc ccgcaccgtc aaacagaaca tccattttgg gaccgaccag cgaaccaaaa
    23041 ataaaggcac ttcctgcgtc actgtaggac atcactttat ggacgcctaa tgccgcctgc
    23101 tctactgccc attttcccgg gggaaaatag agcatgatgc caccaatggc aatttgcagc
    23161 agcagcgcag cgccaaccgt gcgcaagctg atgctctttt tatttaccga caacacaaag
    23221 gcaattgcca gcaataccac catccccgca acacttctca ttatatccat aatgattttc
    23281 ccttcatgcc agtaaacccg gcgtcagcgc caggttttgg tatgtttgat gagtacgggc
    23341 gacggctttc tgcccgccag aaattaaccc acgagacgct gatattcttt ggcaatttcg
    23401 ctcgccagaa tggcgttatt aaagaccagt tgaatattgg atttcaggct atcaccgccg
    23461 gttagctccg caacacgcgc cagcaggaac ggtgtactct ctttcccaat aacaccctgt
    23521 tcttcggctt cagcaacggc ttgatcaatt gctgcattga tggtttcttc tggcatcgca
    23581 aactgttccg ggatcgggtt cgctaccacc aggccgccgt tcaaaccggt ttgccacttc
    23641 actgccatcg cgcgagcaat ttcgcgggca ctgtcaagac gaatgctgac ttcaaatggg
    23701 ctggttcggc agaaaaacgc aggcagcgct tttgtctgat aaccaattaa cggtacaccg
    23761 aaggtttcta aatattctgt tgttaatcct aaatcgagaa tagattttgc ccccgcacaa
    23821 acaacagtga cattggtatt tgccagttcc tgtaaatcgg cagaaatatc gaaggtatgt
    23881 tccgctccgc gatgcacacc accaatacct ccggttgcaa aaactttaat cccagccagt
    23941 gctgcaataa tcatggttga tgcaacggta gtcgcgccat ttttccctgc agcaacgaca
    24001 aaaggtaaat cacgacgact aactttcgtc acgttatgtc cttcacggcc cagcaattcg
    24061 atttcttctt tacttaagcc cactttcatc acaccgccaa taatggcgat agtggctggt
    24121 actgcgccct gtttacgaat tgtttcttct acttcaattg cggtttgggc attttgtggg
    24181 aatggcattc cgtgagaaat tatggtcgat tccagcgcaa caaccggttt tttatttttt
    24241 aaagcatcct gtacttcagc ggatatttgt aataattcag gggacagagt taattcagac
    24301 attctgtgtt ctccactaac gatctaacat tggcaattga taattcaggg ttgttggtat
    24361 attcacaggc aagtgccatt gacgaacatc cctgcgcaaa tcgaacagaa tcgataaacg
    24421 gcataccgtc tacccaacag gaggccagcc ccgccatcat ggcgtcgcct gccccagtaa
    24481 cattgatgac attcgtttta ataggggccg accacccact ttcaccgttg atatcactgt
    24541 agtaaacgcc atcccctccc atgctcagca ccaagcggtt caggccgtgt tgatgaaacc
    24601 aggcggcgac ctttgcgaca tcttcacgtc cggaaagtgc aattccgctc aacgtttcgg
    24661 cttcaagacg gttaggtttt aacgtatgga ttttgctcag atgatcgcgg attttgacgc
    24721 atttccatgc cgataccgga tcaataaata caggcgtttc cccgctattt tccagaaccc
    24781 agaccagcac ctcttcacta aggttgcaat ccgcgacaat aacctttgca ccgcgaataa
    24841 actcctgatg ctgggcaaga aattctgcag aaatacagtc actaatactc atatcattta
    24901 tcgcgacaag catttctcct gtattatcga gcagtgataa atagctggat gtattctcac
    24961 ccggaacaat taagcattta tcgacataaa caccggattg attagtttgc gccagtaatg
    25021 attgcccata aaaatcacca cctactgcac ttaaaagcca ggcatttttc cccagtaacg
    25081 ccagattatg agcgatattc cttcccacgc cacctggggt aaatttaatt tttccgggat
    25141 tcgaatccgc ataattcaat ggatgatgtg aatacccagc cacatcaata ttcgccgaac
    25201 caataattac tatgtagtcc ttttcgcgca tagcacgccc cctggcaata aattgccaac
    25261 gaagaaattg ggtaaaatag ccattacagt caataagact attagagcac gtgttcagat
    25321 ttaaacacat gttcataata agcatattta acgtaatgta aatcgatcag aaaaaacgaa
    25381 atcggccaga tatgaaggcg gtcacaaagt ttgtctattt atgcagcaac aaaagagaaa
    25441 gttgtgatgt gtaacacaca atatggcagg caagaaaaag ggcagagatg tttctgccct
    25501 ccagattgtt gacaacgcgc gctttgttca tcctggatgt ggcgtaaacg ccttatccga
    25561 cctacaaaag cgcaaaaaat tcaatatatt gcagggttca tgtaggtctg ataagcatag
    25621 cgcatcaggc aattttgcgt ttgtcatcat cggggcagag atgtctcatc cccgataatg
    25681 aagataaatt atgcgttttt ttctactatt tgtgtttccg ggcgtttcag gaaggcatag
    25741 gccaaacccg ccaccagcgt accggcaata attgctacca ggtaacccaa taccggcgta
    25801 atagcgccag ggatcagcag aacaaacaga ccaccgtgtg gtgccatcag tttcgcacca
    25861 atcgccattg agattgcgcc agtcagcgcc ccaccaacga tacagcacgg tagcacacgc
    25921 atcggatcac gagcagcaaa cggaattgca ccttccgaaa taaagcacag accaagtacc
    25981 agagcggctt tgccaccttc ctgctgcgct ttgtcgaatt tgcgacgcgc caccattgtt
    26041 gccagaccca ttgccagcgg tggcaccata cctgccgcca taatcgccgc catcgggcca
    26101 taggtttgag tactcagcag acccacaccg aatgcgtacg ctgctttgtt taccggaccg
    26161 cccatgtcag tacacatcat gccaccgagg atcgccccca gcagaaccgc attcgcagtc
    26221 cccatggtct gcagccagtg agtcaaccca tcgagaatgc cagcaaccgg tttaccgatt
    26281 aggtagatca tcgccagacc gaccaccaga ctggaaatta gcggaatgat caggatcggt
    26341 ttcagcgcct ccatactctg tggcagtttc aattgcgtac tgattaactt cgcaatgtaa
    26401 ccagccagga agccagcaat tataccgcca ataaagccag aaccggtgct gaccgccagc
    26461 ataccgccaa tcagacccgg tgtgaggccc ggacgatcgg caatggagaa ggcaatataa
    26521 cctgccagta ccggcaccat cagcgcaaag gctgaaccac caccaatctg catcagcgcc
    26581 gctgccaacg tacccggctc tttaaacgct tcgataccaa aagcaaaaga aagcgcgata
    26641 cacagaccac ctgcaacaac catcggcagc atgtaagaga cgcccgtcag caagtgacgg
    26701 taagcgcctg cactctcttt cttaccttca gtggtcgctg tttgagcttt gcccgctggt
    26761 tcatacggcg ttgcttcagc aaccgcttta tccagttcct gcgcagtttt cttcagcgcc
    26821 agaccggtag aggtgcgata catcggttta ccagcaaatt tcgccagatc cacttcgata
    26881 tctgccgcca caatcaccag atccgctgct gcgacttctt caggggtgat tgcattgccc
    26941 gcgccaacag aaccacgggt ttcaactttc acccaccagc cacgtttttt cgcttcggtt
    27001 tcaatggctt cagccgccat aaaggtgtgt gctacgccag tcgggcaagc agtcaccgca
    27061 actacgcgtt tcggaccgct ggcagcaacc ggtgctgtcg cagcgaccgg cgcagtgtaa
    27121 ggtttcgcat ggcctttggc ttcactcagg aacagctcag ggtgcgcaac tgcccgggaa
    27181 atatcaccca gccagacatt tttaccgttc agcgcgctgt cattcgggat ggaatcaccg
    27241 agaacaatcg ccatttctgc gtcgttcgga ttgtcgatga tttccagttt tgcttttcgc
    27301 gccgccgcgc ccagcagggt cttcgccata taggcgcgtg cctgaccgag attagcgtca
    27361 ataatcagca gcgttttcat tatgcctctc ctgctgtcag ttaaaaggtt gtaagtcgac
    27421 gcgcgccatc attgcggcca actgcggacg atcggtaata cccacattgc tttgacttac
    27481 cgccagggct gcaacagctg tcgccagacg cagtgtgtgt tcactggatt cacgcatcag
    27541 caagccataa atcaggccac caaccataga atcccctgcg ccaacggtgc ttacgacatc
    27601 gactgacggt ggtttggcga tccattcgcc ggaggcatta acccaaagcg cgccttcggc
    27661 acccagtgaa ataacaacat gcgcaatacc ttgttcacgt agcgcatgtg cagcttcaat
    27721 cacatctttc atttcaggca gtttacggcc tgcccagatt tccaactcgc ggcggttagg
    27781 tttcaccagc cacggtgccg ctttcaaacc tgctactaac gcttcacggc tactatcaaa
    27841 gataatgcaa ggacactgac tacgcaggcg agtcatccag tcggtgaacg cttccgggct
    27901 gacgcctgac ggtaagcttc cgctgacaca gaccatatcg aactgaccga gccagctcag
    27961 agaatcagtc acaaagcgtt cccagtcggc gagggtgact tcaaaacccg agaagttgaa
    28021 gtcggtcact tcgccgtctt tttccgtcag cttaacgtta attcgggtgc gcccctgtac
    28081 aacctggaaa cggttggcaa tgcccagctc gctgaacagt tgctgaaaac catcctgatt
    28141 gtctttaccc aggaagccgc caacggtgac atcaattccc aggtctttta atactttggc
    28201 cacgttgatg cctttacccg ccgcatgcag accggtggtt ttcaccaggt tcacttcgcc
    28261 gcgttcaatt tccgggcaga aaccaacaag gtcataagcc ggattaaggg tgatcgtagc
    28321 aacacgtctg ctcattatgc gccctcccca agaccagcag cgatagcgtc gccaatggct
    28381 ttcagcgcct gttcagcatc tgcaccctgg gcggtaaagc gtaggcgatg acctttctta
    28441 acgccaagtg ccacaacttt catcagacta cgtccgtttg ccggtttgcc ggtgccatca
    28501 aggtttgtca cggtaatatc actgttaaat tgtttaatgg tattgaccag catggtacct
    28561 ggacgagcat gcaggccgtg ttcattgcgc accacaaact ccgcgcttaa cacgtcgtcg
    28621 gtcggcgcat catcgctggt cagcagcgcc agcaacgttg ccgcatccgc tttcagcaag
    28681 cggtcagctt tattgtcgag caataaatca gcgagacgct taagaaccgc gatgggctga
    28741 tcgtcattca tcgccacact caccagcatg gctgccgttt cgccgtccac atcaaaagca
    28801 tttgccgcac ggcttaccgc aatcgcgcta cgcagattgc cttcggcgct atcgctcagc
    28861 cagataccct gtccgagatt cagcggttgt tcattgatgg ctttggtgac gaaagtggcg
    28921 tcaactgccc ccacctcttt cagacgcgca gcgttcagcg cctgaagagt cagcagatcg
    28981 ctggcgacga tatccagcgt cagcatttcg ttgtcgagct tcagctgctc actctgcttt
    29041 tcgcccatca gtaatgcgcg aagttcttct gctgttgttg ctgacttcag ttgttcagca
    29101 acggaatcat cgctcagtac gtgggtcagc tggcgtagca ggcccagatg ttcatccgag
    29161 ctggcagcaa taccgattgc cacgtacgct acctgaccgt caccccaggt gacgccttcc
    29221 gggaactgaa atacctgaac gccggttttc agcacctgat cgcgggtgtc ggtagtgccg
    29281 tgtggaatag caataccatt gccgaggaac gttgaggttt gctgttcgcg cgccagcatg
    29341 ccattgacgt agccttctgc tacattaccg gcctgcacca gcgccgcagc gacctggcga
    29401 atcgcctctt ctttgtctcc ggccttttcg cccggatgga tgtcctgtac ggataactgg
    29461 aacatagttc tcctctcttg ctgaattgaa acgattcagc ctctatgaga aaaaaagcgc
    29521 caacctggct tagggttaaa gacaagatcg cgctgaaacg tttcaagaaa gcataatact
    29581 tctgtttcag cacgcaagga aagccgtaaa gttatgagca aaagtttgat gtgctgcaca
    29641 attttttgtc atttttccta attgctgatg ggaaaatccg ttatcagcaa tttcatttca
    29701 gcagcgtcag cagccaatca gcaacagtga aatggcattt tgattattcg tggcgctttt
    29761 gatttcgctg tctgtttatt ttctgacaag cagcgtaaac tccgcgtctt cctcttccag
    29821 tgatcgacct gcatgcataa ctcccccgca gtctccagcg cgaaatcgtt tgacctgacc
    29881 tcgacggcgt ttttaatcgt tgcctttctc accggtattg cgggtgctct gcaaaccccg
    29941 acactcagta ttttccttac cgatgaagta catgcccgtc cggcgatggt gggattcttc
    30001 tttaccggca gcgctgtcat tgggattctg gtaagtcagt ttctcgctgg gcgctctgat
    30061 aagcgcggcg atcgcaaatc gctgattgtc ttttgctgcc tgttaggcgt gctggcctgc
    30121 accctttttg cctggaatcg caactacttt gttttgctat tcgttggcgt ctttcttagc
    30181 agctttggct cgaccgctaa cccgcaaatg tttgcccttg cccgtgaaca tgccgacaaa
    30241 accggacgtg aggcggtgat gttcagctct tttttacgcg ctcaggtttc actggcatgg
    30301 gtcattggcc caccgctggc ttatgcgtta gcgatgggtt tcagctttac ggtaatgtat
    30361 ctgagcgcag cggtagcgtt tattgtttgc ggcgtgatgg tgtggctgtt tttaccgtcg
    30421 atgcaaaaag agcttccgct ggcgaccggc acggttgaag cgccgcgccg taaccgtcgc
    30481 gatacgctac tgctgtttgt catttgtaca ttgatgtggg gctcgaacag cctgtacatc
    30541 atcaacatgc cgctatttat tatcaacgaa ctgcatcttc ccgagaaact ggccgatgtg
    30601 atgatgggga ccgccgccgg gctggaaatc ccgacgatgt tgattgccgg atatttcgcc
    30661 aaacgtctgg gtaagcgttt cttaatgcgc gttgctgccg tgggtggcgt ctgtttttac
    30721 gcaggaatgc tgatggcgca ttcacctgtc attctgttgg gcttgcagct gctaaatgct
    30781 atttttattg gcattctggg cggcatcggg atgctctatt ttcaggatct gatgcccggt
    30841 caggcgggtt cagccaccac gctctatacc aacacttcgc gcgtgggctg gatcatcgca
    30901 ggatcagtgg cgggcatcgt cgccgaaatc tggaattatc acgctgtgtt ctggtttgcg
    30961 atggtgatga ttatcgccac tctgttttgc ttactgcgga ttaaagatgt ttaaggcgcg
    31021 gtcagcattt ccagatcgat aagccaggtc atggcctgct gacgcgagtt accgcacatt
    31081 tcagcgctgg cttgtaagcc tgcacagact ttcggtcgca gtggcgaggt gaaaatttta
    31141 cagcgttgct gttcatcaag ctgaatgcag ggcgtattgg cgggcttgcc atctggcata
    31201 ccgggaatag ggctggaaat tgaaggggca gtgcaacacg ccccgcaacc cggacggcat
    31261 tccatacgtt gttctcttaa ggttaaccac cggattgatg agcgcgcaca gtaccacctt
    31321 ttttgcacca gcaaaagtgc gaataccact tgcccgaaag gcccgtcgcg agtactttgt
    31381 cgcgatattt ttgacatttt cgactacagg aatttttcga tgccaagagc gaacgaaatt
    31441 aaaaaaggta tggtactgaa ttacaacggc aaactgctgc tggttaagga tattgatatt
    31501 cagtcgccca ctgcccgcgg tgccgctacg ctgtacaaaa tgcgtttttc tgatgtccgt
    31561 accgggctga aagtagaaga gcgtttcaaa ggtgatgata tcgttgacac cgtgacgctg
    31621 acccgccgtt acgttgactt ctcctatgtc gatggcaacg aatatgtctt tatggataaa
    31681 gaagactata ccccgtatac cttcaccaaa gatcagattg aagaagagtt gctgtttatg
    31741 ccagagggcg gcatgccgga catgcaggtg ctgacctggg acggtcaact gctggcgctt
    31801 gagctgccgc agaccgttga tctggaaatc gttgaaaccg caccaggcat caaaggggca
    31861 tctgccagcg cccgtaacaa accggcgaca ttgagcactg gtctggtgat tcaggtacca
    31921 gaatacttaa gcccgggcga aaaaattcgt atccatatcg aagaacgccg ttatatgggg
    31981 cgtgctgact aacttcagcc gcatgcagaa aagggatagc tcaggctgtc ccttttttaa
    32041 tttattatac caatcttcta ttttgcgctt cctgaacacc ttacgccctc aattttcact
    32101 cgttgattga tcgccctcac acttcatcgc attaacaatc cagaccaatt tcaattgctg
    32161 tcatataact ttacactgtc attgttaatt tatcgttact aagacgtgac tcctatgaag
    32221 acaattgcct ccgttacgct cccgcatcat gtacacgctc cacgctacga tcgccagcag
    32281 ttgcaatcac gtatcgttca ttttggcttt ggcgcctttc accgcgctca tcaggcgtta
    32341 ctgaccgatc gtgtgctgaa tgcccagggc ggcgactggg ggatctgtga aatcagcttg
    32401 ttcagcggtg atcaactgat gagccagctc cgcgcacaga accatttata taccgtgctg
    32461 gagaaaggtg cggacggcaa tcaggtgata attgtcggtg ccgttcacga atgccttaat
    32521 gcaaaactgg attccttagc ggcaattatt gagaaatttt gcgagccaca ggtggcaatt
    32581 gtttccctga cgattaccga aaaaggctat tgtattgacc cggccaccgg tgcactcgac
    32641 accagtaatc cgcggattat tcacgatcta caaacccctg aagaacctca ctccgcaccg
    32701 ggtattctcg tcgaagcact gaaacgccgc cgtgagcgcg gccttacacc gtttaccgtg
    32761 ctctcctgcg acaatattcc cgacaatggc catgtggtga aaaacgcggt gctgggaatg
    32821 gcagaaaaat gttcgccaga actcgccggg tggataaaaa aacacgtcag ttttccggga
    32881 accatggtcg accgcattgt tccggctgca accgacgaat cactggcaga aatcagccag
    32941 catctggggg taaatgatcc ctgcgcgatt agctgcgaac cgtttatcca gtgggtggtt
    33001 gaagataact tcatcgctgg gcgtcctgcc tgggaagtcg caggtgtaca aatggtgaat
    33061 gatgtcctgc catgggaaga gatgaaactg cgtatgctta atggcagcca ctcttttctc
    33121 gcttatctgg gttacctctc aggattcgtc catatcagtg attgcatgca ggatcgcgta
    33181 tttcgccatg ctgccagaac attaatgctg gatgagcaag cgccgacact gcaaattaaa
    33241 gatgtcgatt taacacaata tgcggataag ttaattgcac gttttgctaa tccgtcgctg
    33301 aaacataaga cctggcaaat cgcgatggat ggcagccaga aattaccgca acgtatgctg
    33361 gcaggtattc gcatacatct ggggcgtgaa acggactggt cgttgctggc attaggcgtt
    33421 gctggctgga tgcgttacgt cagcggcgtt gatgatgccg gaaatgccat tgatgttcgc
    33481 gatccgctta gcgataaaat tcgcgaactt gttgcgggca gcagcagtga acaacgcgta
    33541 accgccctgc tttccctgcg tgaagttttc ggtgatgatc tgccagataa cccgcatttt
    33601 gtgcaggcca tcgaacaagc ctggcaacaa atcgtacaat tcggcgcaca tcaggcgcta
    33661 ttaaacaccc tcaaaattta acgatttctg cggttaaagc ggatgaagct caccttcgtc
    33721 cgctctcccc ttctcttttc tgcctttttt agccaggatt aacgctcagt taacttacca
    33781 gagtattgcg gagccgttgt gaccaggacc aacctcatca ccggttttct cggcagcggg
    33841 aaaaccacgt cgattcttca tctgttagcc cataaagatc ccaacgaaaa atgggcggta
    33901 ctggttaatg aatttgggga agtcggaatt gatggtgctt tgctcgccga tagcggcgca
    33961 ttgctgaaag agatccccgg cggctgcatg tgctgcgtta atggtttacc catgcaggta
    34021 gggttgaata ccttactgcg tcagggaaaa ccagaccgct tgttgataga gccgaccggg
    34081 ctgggccatc cgaaacagat cctcgatctg ttaaccgcac cagtctatga actgtggata
    34141 gatctgcgcg ccaccttgtg cattctcgat ccacgcctgc tgctggacga aaaaagcgcc
    34201 agcaatgaaa acttccgtga ccagctggct gccgcagaca tcattgtcgc caataaatcc
    34261 gaccgtgcga cgcccgaaag tgagcaagcg ctacagcgtt ggtggcagca aaatggtggc
    34321 gatcgacaat taattcacag tgagcatggg aaagttgacg gtcatcttct ggatttgccg
    34381 cgtcgcaatt tagccgagtt gcccgccagc gccgcgcatt ctcatcagca tgtcgtgaaa
    34441 aaagggttag cagcgttaag cctgccagag catcaacgct ggcgtcgcag tctgaacagc
    34501 gggcaaggat atcaggcctg cggctggata ttcgacgctg atacggtatt cgacaccatt
    34561 ggcattctgg aatgggcgcg acttgcaccg gtggaacgcg tcaaaggcgt gctgcgtatt
    34621 cccgaagggc tggtacgaat caaccgtcag ggcgatgacc tgcacattga aacgcaaaac
    34681 gttgcgccac cggacagccg tattgagctg atttccagca gcgaagctga ctggaatgct
    34741 ttgcagagcg cgctgttgaa gcttcgttta gcgactaccg cgtaaggttg cctgcgtttt
    34801 tcagtaagat aattagagaa aatatgatta aaaatttgcc gcaaatagtg ttgttgaata
    34861 ttgtcggcct cgcgctgttt ctttcctggt atattcccgt taatcatgga ttctggttgc
    34921 cgattgatgc ggatattttt tatttcttta atcagaaact ggtcgaaagt aaggcctttt
    34981 tgtggctggt tgcattgacc aacaatcgcg ctttcgacgg ttgttctctg ctggcgatgg
    35041 gaatgttgat gctgagtttc tggctgaaag aaaacgcccc tggcagacga cgtatcgtga
    35101 ttatgggtct ggtcatgcta ttaactgcag tggtattaaa ccagctgggt caggcattaa
    35161 ttcctgtaaa acgggccagc ccaacattga cttttaccga tattaaccgc gtcagcgaac
    35221 tgctctctgt tcccacgaaa gatgcctcac gagatagctt tcccggcgat cacggcatga
    35281 tgctgcttat tttttcggca ttcatgtggc gttatttcgg caaagttgca ggccttatcg
    35341 cccttattat ttttgtggtt tttgcatttc ccagagtaat gattggcgca cactggttta
    35401 ctgacatcat tgtcggttcg atgaccgtga tattgatcgg tttgccctgg gtgttgctga
    35461 cgccattaag tgatcgatta atcacctttt ttgacaaatc actaccggga aaaaacaaac
    35521 atttccaaaa caaataactc acagtaatta acatcatcag gcttattttt atagtgagga
    35581 taatcctgat gatgcgcacc gtgctttcat ccatcgcgcg caaaaatcat tctctaagta
    35641 aatgaatgga ttgcatgcgt ttcactcaat cgtactttaa ttgaccaatc ccgcttatta
    35701 actttctgta tcactttttc ttataaaaaa tcatgtaaaa ccgctcgcca agaccgcacc
    35761 aatcgggtaa tctagaactc gttttgcctc ggcggtagat tatcctcaca gcatataatt
    35821 ttgtgcgtta gtccacagat ttggccttaa ggaattgttt caacatgccc aggtaattag
    35881 tctcgtgtcg cttggcattt ttttataacg atatttgtcg ttaaggactt caagggaaaa
    35941 caaacaacat ggtcaaatct caaccgattt tgagatatat cttgcgcggg atccccgcga
    36001 ttgcagtagc ggttctgctt tctgcatgta gtgcaaataa caccgcaaag aatatgcatc
    36061 ctgagacacg tgcagtgggt agtgaaacat catcactgca agcttctcag gatgaatttg
    36121 aaaacctggt tcgtaatgtc gacgtaaaat cgcgaattat ggatcagtat gctgactgga
    36181 aaggcgtacg ttatcgtctg ggcggcagca ctaaaaaagg tatcgattgt tctggttttg
    36241 tacaacgtac attccgtgag caatttggct tagaacttcc gcgttcgact tacgaacagc
    36301 aggaaatggg taaatctgtt tcccgcagta atttgcgtac gggtgattta gttctgttcc
    36361 gtgccggttc aacgggacgc catgtcggta tttatatcgg caacaatcag tttgtccatg
    36421 cttccaccag cagtggtgtt attatttcca gcatgaatga accgtactgg aagaagcgtt
    36481 acaacgaagc acgccgggtt ctcagccgca gctaataaac cgtttggatg caatcccttg
    36541 gctatcctga cgagttaact gaaagcactg cttaggcagt gcttttttgt tttcattcat
    36601 cagagaaaat gatgtttccg cgtcttgatc caggctatag tccggtcatt gttatctttt
    36661 aaatgttgtc gtaatttcag gaaattaacg gaatcatgtt catacgcgct cccaattctg
    36721 gacgtaagct cctgcttacc tgcattgtag caggcgtgat gattgcgata ctggttagct
    36781 gccttcagtt tttagtggcc tggcataagc acgaagtcaa atacgacaca ctgattatcg
    36841 acgtacaaaa gtatctcgat acctattttg ccgacctgaa atccactact gaccggcttc
    36901 agccgctgac cttagatacc tgccagcagg ctaaccccga actgaccgct cgcgcggcgt
    36961 ttagcatgaa tgtccgtacg tttgtgctgg tgaaagataa aaaaacattc tgttcatctg
    37021 cgactggtga gatggacatt ccactcaatg aattgattcc ggcgctcaac attaataaaa
    37081 acgtcgatat ggcgatctta cccggcacgc cgatggtgcc gaacaaaccc gcaatcgtca
    37141 tctggtatcg caaccctttg ctgaaaaata gcggtgtgtt tgccgctctg aatctcaacc
    37201 tgacgccttc tctcttttat agttcacggc aggaagatta cgatggcgtc gccctcatta
    37261 ttggcaatac tgcgctatct accttttctt cacgtttgat gaacgttaat gaattaaccg
    37321 acatgccggt ccgtgaaact aaaattgcgg gcattcctct gaccgttcgg ctttatgcgg
    37381 atgactggac atggaacgat gtgtggtacg catttttact gggcggcatg agtggaactg
    37441 tcgttggcct gctctgctat tacctgatga gcgtacgtat gcgccccggc agagaaatca
    37501 tgaccgccat caagcgcgaa caattttacg tggcgtatca accggtggtg gatacacaag
    37561 ctttgcgagt aacgggcctg gaagtactgc tacgctggcg gcatcctgtc gcgggagaaa
    37621 ttcccccgga tgccttcatt aactttgccg aatcgcaaaa gatgattgtg ccgctgactc
    37681 agcacctgtt tgagttaatt gcccgcgatg ccgcagaatt agaaaaagtg ctgccagtag
    37741 gcgtcaaatt tggtattaac attgcgccgg accatctgca cagcgaaagc tttaaagcag
    37801 atatccagaa actgctcact tccctgcccg cacaccattt ccagattgtg ctggaaatta
    37861 ccgagcgcga tatgctgaaa gagcaagaag ccacacaact cttcgcctgg ctgcactcgg
    37921 tcggcgtaga aattgctatt gatgacttcg gcaccgggca cagcgcgctt atctatcttg
    37981 agcgttttac gctcgattat ctgaaaattg accgtggatt tatcaacgcc atcggtacgg
    38041 aaacgatcac ttcacccgta cttgacgcgg tgctgacgct ggcgaaacgc ctcaatatgc
    38101 tgacggttgc tgaaggggtc gaaacgccgg aacaggcgcg atggctaagc gaacgcggcg
    38161 ttaatttcat gcaaggctac tggattagtc gccctttacc gctggacgat tttgtccgct
    38221 ggctgaagaa accgtatacg ccgcagtggt aaggtgtgct tacgtccctt attattcata
    38281 gtgaaagcat gccggattgc ggctaatgat gagtaaaagg aaatccattg cagatgattg
    38341 tgcgcatact gctgctgttt atcgctctgt tcacctttgg tgtgcaggcg caggctatca
    38401 aggaaagcta tgcctttgcc gtgctgggcg aaccccggta cgcgtttaat ttcaaccatt
    38461 ttgattatgt gaaccccgcc gcgccaaaag gtgggcagat aacgttgtca gccctcggca
    38521 ccttcgataa tttcaaccgc tatgcactgc gcggcaaccc gggcgcacgc accgagcagc
    38581 tgtacgacac gctatttacg acttccgatg acgaaccagg cagttattac ccgctgattg
    38641 ctgaaagcgc acgctatgct gacgattatt cctgggtgga ggtcgctatt aatccgcgcg
    38701 cccgttttca tgatggttcg cccattactg cccgcgatgt agagtttact tttcaaaaat
    38761 ttatgaccga aggcgtgccg caatttcgtc tggtctacaa aggcaccacc gtcaaagcca
    38821 ttgcaccgtt aaccgtgcgc attgagttag ctaaacccag caaagaagat atgctgagtc
    38881 tgttttcgct gccgatattt ccagaaaagt actggaagga tcacaaactt agcgacccgc
    38941 tcgccacgcc tccgcttgcc agtggtccgt accgcgttac gtcctggaaa atggggcaaa
    39001 atattgtcta ttcccgcgtg aaagattact gggcagcaaa cttaccggta aaccgtggac
    39061 gctggaattt cgacaccatt cgctacgatt attacctcga tgataatgtc gcctttgaag
    39121 cgtttaaagc aggtgccttt gatttgcgta tggaaaacga cgctaaaaac tgggccacgc
    39181 gttataccgg taaaaatttc gataaaaaat acatcatcaa agatgagcaa aagaacgaat
    39241 cagcccagga tacgcgctgg ctggcgttta atatccaacg tccggtattc agcgatcgcc
    39301 gggtccggga agcaatcact ctcgcctttg actttgaatg gatgaacaag gcgttgtttt
    39361 acaacgcctg gagtcgcacg aacagttatt ttcagaatac cgaatacgcg gccagaaatt
    39421 accccgacgc cgcggagctg gtgcttctgg caccaatgaa aaaagatcta ccgccagaag
    39481 tcttcacaca aatctaccag ccgccggtat ctaaaggcga tggctacgat cgtgacaacc
    39541 tgttaaaagc cgacaaactt ctcaacgaaa cgggctgggt gctgaagggt cagcaacgcg
    39601 ttaatgccac aacgggtcag ccgctcagct ttgaattatt gcttcccgca agcagcaata
    39661 gtcagtgggt attgccgttc cagcacagcc tgcaacggct gggtatcaat atggacattc
    39721 gcaaggtgga taactcgcaa atcactaacc gcatgcgcag tcgcgactat gacatgatgc
    39781 cgcgcctatg gcgggcgatg ccgtggccca gttccgattt acagatttcc tggtcatcgg
    39841 aatatatcaa ttccacttat aatgcccccg gcgtgcaaag cccggttatc gactcgctga
    39901 tcaaccaaat tattgccgcg cagggaaata aagaaaaatt actgccgttg gggcgagcac
    39961 tggatcgcgt attaacgtgg aattattaca tgctgccaat gtggtacatg gcggaagacc
    40021 gtctcgcctg gtgggataaa ttctcccaac ccgctgtacg ccctgtttac agcctgggta
    40081 tcgatacctg gtggtatgac gttaataaag cggccaaact gccgtcagcc agacaacagg
    40141 gagagtagat gggcgcttac ctgattcgcc gtctgttgct ggtgatccca acgttatggg
    40201 ctattatcac tatcaacttt ttcatcgtgc aaattgcgcc tggcggtccg gtcgatcagg
    40261 ccatcgccgc cattgagttt ggtaatgccg gagtattacc cggcgcaggc ggtgaaggtg
    40321 ttcgtgccag ccatgcgcaa acgggtgtcg gcaatatcag cgacagtaat taccgtggcg
    40381 gacgcggatt agacccagaa gtgatcgctg agatcactca tcgctacggt ttcgataagc
    40441 cgatccacga acgttacttc aaaatgctct gggactacat ccgctttgat tttggtgata
    40501 gcctgtttcg cagcgcctcg gtgctgacgc tgattaaaga cagtctgccg gtttccatca
    40561 ccctcggatt gtggagcacg ctgattatct atctggtgtc gattccgtta ggcattcgca
    40621 aagctgttta taatgggagt cgctttgacg tctggagtag cgcatttatc atcatcggct
    40681 acgccattcc ggcctttttg tttgccatcc tgctgattgt cttcttcgcg ggcggcagct
    40741 atttcgacct gttccctcta cgcggcctgg tttccgctaa ctttgattcg ctgccgtggt
    40801 atcagaaaat caccgattat ctgtggcata tcacgctacc ggtgctagcg acagtgattg
    40861 gtggctttgc ggcgctgacc atgctgacca aaaactcatt ccttgatgaa gtgcgcaagc
    40921 aatacgtggt gaccgcgcgt gcgaaagggg taagtgagaa aaacattctc tggaaacatg
    40981 tgttccgcaa cgccatgctg ctggtgattg ccggttttcc ggcgacgttt atcagcatgt
    41041 tttttaccgg ctcgctgctg attgaggtga tgttttcact caatggtctt ggcttactgg
    41101 gctacgaagc gaccgtctcg cgcgattatc ctgtaatgtt tggtaccttg tatattttca
    41161 ccctgattgg cctgctgctg aatattgtca gtgatatcag ctatacgctg gttgatccgc
    41221 gtatagattt tgagggacgt taatgtcgcg actcagcccc gtcaatcagg cccgttgggc
    41281 gcgttttcgt cataaccgtc gcggctactg gtcgttatgg attttcctcg ttttgtttgg
    41341 tttgagtttg tgttctgaac ttatcgctaa cgataaaccg ttgctggtgc gttatgacgg
    41401 cagttggtat ttcccgttgt tgaaaaacta cagcgaaagc gattttggcg gcccgctggc
    41461 aagtcaggct gattatcagg acccgtggct gaaacaacgg ctggaaaata acggctgggt
    41521 actgtgggca ccgattcgct ttggtgctac cagtatcaac tttgctaccg ataagccctt
    41581 cccttctcca ccctcccggc aaaactggct gggaacggat gccaacggcg gcgatgtgct
    41641 ggcacgcatt ctctatggca cgcggatctc ggttctgttt ggcctgatgc tgactctctg
    41701 ttccagcgtg atgggcgtgc tggcgggggc gctacaaggc tattacggcg gtaaagtcga
    41761 tctctgggga caacgcttta ttgaagtatg gtcggggatg ccgacgctgt ttttgattat
    41821 tttactttcc agcgtcgtac agcctaactt ctggtggctg ctggcaatta ctgtcttgtt
    41881 tggctggatg agtctggtcg gcgtggtgcg ggcggagttt ttacgtactc gtaatttcga
    41941 ctacattcgt gcggcacagg cgcttggcgt cagcgatcgc agtatcatcc tgcgtcatat
    42001 gttgcctaat gccatggtcg cgaccctcac ctttttaccg tttattttat gtagttcgat
    42061 aaccaccctg acctcgctcg atttcctcgg cttcggtcta ccgctcggtt caccgtcact
    42121 cggtgaactg ctgttacaag ggaaaaataa ccttcaggcc ccgtggcttg ggatcaccgc
    42181 cttcttgtcg gtggcgatat tattgtcttt gctgatcttt attggtgaag ccgtccgcga
    42241 cgcatttgat cctaataagg cggtgtagca tgacgcaaac tctgttagcg attgaaaatt
    42301 tgtcggtggg ttttcgccat cagcaaaccg tacgtacagt agtcaatgat gtttcactac
    42361 agattgaggc tggcgaaacg ctggcgctgg tgggtgagtc aggttcaggc aaaagcgtta
    42421 ccgcgctgtc aattttacgc ctgctccctt ccccgccggt tgaatatctc tccggcgata
    42481 ttcgttttca tggcgaatcg ctgcttcatg ccagcgatca aacgttgcgc ggtgtacgcg
    42541 gtaataagat cgccatgatt tttcaggaac cgatggtgtc gttaaatcca ttgcataccc
    42601 tggaaaaaca gctttatgaa gtgctttcac tccaccgcgg gatgcgtcgg gaagcggctc
    42661 gtggcgaaat tcttaactgc cttgatcgcg tcggtatccg ccaggcggca aaacggctga
    42721 cagattatcc gcatcagctc tccggcggcg aacgccagcg ggtgatgatt gcgatggcgc
    42781 tgttaacgcg accggaatta ttaattgccg atgaaccgac caccgcactg gacgtctctg
    42841 tccaggcgca gattttacag ctgttgcgcg aactgcaagg cgagctgaat atgggcatgc
    42901 tgtttattac tcataacctc agcattgtca gaaaactggc ccaccgcgtg gcggtaatgc
    42961 aaaacggtcg ctgtgtcgag caaaataacg ccgctacgct atttgcctca cccactcatc
    43021 cttacacaca aaagctactc aacagtgaac cgtcaggcga tccggtaccg ttgccagaac
    43081 ctgcctcaac gttgctggat gttgaacagc ttcaggttgc cttccccatt cgcaaaggga
    43141 ttttgaagcg cattgtggat cataatgtgg tggtgaaaaa catcagtttt acgctacggg
    43201 cgggtgaaac actgggttta gtgggcgagt ctggttccgg gaaaagcacg acgggactgg
    43261 cgctgctgcg actgattacc tctcagggca gcatcgtctt tgacggtcag ccactgcaag
    43321 atttaaatcg ccgccagctg ttacctactc gtcatcgtat tcaggtggta tttcaggatc
    43381 caaactcctc gctcaaccca cgactcaacg ttttgcagat tattgaggaa ggcttacggg
    43441 ttcaccagcc gacgctttct gccgcacaac gcgaacaaca agtgatagcc gtgatgcatg
    43501 aagtgggatt agatcctgaa acacgccacc gttatccggc ggagttctct ggtggtcagc
    43561 gacaacgtat tgcgattgcc agggcattaa ttcttaagcc ctcactgatc atacttgatg
    43621 aaccgacatc atcactcgac aaaacggtac aggcgcaaat attgacgcta ttgaaatcat
    43681 tgcaacaaaa gcatcaactg gcctatttgt ttatcagcca cgatttgcac gttgtccgcg
    43741 cgttatgtca tcaggttatc gtactgcgac aaggggaagt agtggaacaa ggaccgtgcg
    43801 cgcgcgtgtt tgccgcaccg cagcaggagt atacgcgtca gctactggcg ttgagctgac
    43861 gcttaaaaag gattgtagtt tgaaaagggt tcggcaattg ccacaccaaa atttttcaat
    43921 cggcatgttg cagcaaactc gtcctggcga tttacaaaca ggcacggctc accttcacac
    43981 tccagcaccg aacatggaac ttcgatgtcg cttaacgcct ggcttagttt atacatcacc
    44041 gaccatgcat tatcaccatc cgggctaagc aatttaagcg ccaccaggct gttgtcaccg
    44101 cacggtccat tttgcggaat cggttcaacc ttcgaacctg cgaaaccagc agaccagcga
    44161 aagttctgcg gtagacgatg gacgattgag agttgtaatg aagtcactta ttttccccgg
    44221 aagcacattt acttcacaat ttatttacat caattttaac acatcatcaa caatccgtct
    44281 ttatacagat cgtaaagatt gatgcctcgc ctgcatggcc tctatggctc tgatttaagt
    44341 aatatctgcg ggcgtggtca cgtttgcggg gctatatgta cccgtttctg cgatctaact
    44401 caaccttttt aactacaatg atgtgacttt ttacataaat tgattttaca taaaataaac
    44461 atatatcggg ggaatgatag atttgtggtt gacatgcatc aacaaaggaa gccttttagc
    44521 ttcctcgttg tgcaatagat caccgttttt tcggtcgact ggcgtacaga cagaagagaa
    44581 tggagctggt tgcgcagaat gcaattgacc aaatcatcgg ccatgcagag ttaaaggtcg
    44641 caagagaaag caatgcgcca acaattgccc ctatgccaaa acggaaggtt cctgccagcg
    44701 aagatgccgt tcccgccata tggggaaact catcaagaat gaccgccatc gcattggatg
    44761 acaccataga cacgcagccc acaaacgccg caacgccaac caccagcgac cagaatccca
    44821 gccccagcag cgcactgatg accatccacg ctgccataat aaattgtatc cacaaccccg
    44881 agcggaacat atttaacgcg ccaatgcggc ggacgaagcg gctgttaaag atggtcatca
    44941 cgaacagaaa aacaatgttt agcgcaaagt aataaccaaa gttttccggt gcaacgtggt
    45001 tgatttcaat gtagacaaac ggtccggcgc tcaggaacga gaacattccg gcaaagctga
    45061 aaccgctggc cagcatataa ctcaggacac gcttatgacg gaacagcgcc gcaaagttac
    45121 caatagtggt acgaatgtga aaaggctggc gaggatccag aagatgtaat gccagctcag
    45181 ccacaccagc acccagccgc caactatcgg tgccatcagc ggtgcaatgg tcgtcaccag
    45241 catgacaaac gacatcatcc gcgagaactc ttctttcggg taaatatcac gcatcaaagc
    45301 gttgataacc acgctggccg cagccgcagc cagcccgtgg aagaaacgca tcacaatcag
    45361 ctgatcgatg gtttgtgcca acgcacacgc caccgcggcg gcggcaaaca ccagcgtacc
    45421 gccaagaacc accggcttac gcccgaagct gtctgccatc ggcccgtaga ttaactgccc
    45481 caacgcaaag cccagaatat aagtactcag ggtcatctgc gtactgcccg ccggtacgcc
    45541 aaactgcgct gaaattaccg gtagcgcggg cagatacata tcaatcgaca gcggcatcaa
    45601 catggccagc aggccaagga taaaaacaat agcaaacgac gaatgctgtc gggtggtcac
    45661 aacgggctcc tgaaagtcat tgaaaagtta gacgacgctg gcaatttctt cttcagttaa
    45721 cggacgatat tcaccggggg ctaaatcagc atccagcgta atgccgccaa tacgttcacg
    45781 atgcagctca accacatggt tacccacggc ggcgaacatg cgtttcacct gatgataacg
    45841 cccttcgctg atggtcagac gaacctgcgt tggggtaatc acttccagca ccgccggctt
    45901 agtgagatct ttttcgttat gcagctgcac gcctttagca aattgttctg ccgtatcgtc
    45961 agctacgggt gataccagcg tcaccagata ggtcttctcg caatgatggc gcggagaagt
    46021 aatgcggtgc gaccactgac catcatctgt cattagtacc agaccggtgg tatcgatatc
    46081 cagccgccct gcggcgtgca gtttccacgc taccggttca tctagaaaat agagcaccgt
    46141 tgggtgatca gggtcgtccg tggagcaaac ataaccctga ggcttattga gcatgaagta
    46201 acgtggaccg tgttgctgcg ccagcgggtt gccatcgtaa gcgacatcat gttcaggaag
    46261 cagtttgaac gctgcattac ggacgatttc gccatcgacg gtgacacgat tgccgcggat
    46321 ttcacgcccg gcaatagcac ggctaacgcc gagttgctgt gcgataaatt tatcaagtcg
    46381 catgtgtgtg attttgcctg taaaaaacgg aggtcgggta atgacccgaa tatctgaact
    46441 gttgtctgcc cagtatagcg gtctaataac atccctcaag ggaaaaagat ccgtggcata
    46501 ctattagcag aataatctac ctacgcgaga ccatgatttt tacacttcgc ccatatcagc
    46561 aagaagccgt ggatgccacg ctcaaccatt ttcgtcgtca taaaacccct gccgttatcg
    46621 tgctgcccac cggtgcaggt aaaagcctgg tgatagcgga actggcacgg ctggctcgtg
    46681 gtcgcgtact ggtgctggca cacgttaaag aactggtggc gcaaaaccat gcaaagtatc
    46741 aggcgctggg gctggaagcc gatatttttg ccgccgggct gaagcgtaaa gagagccacg
    46801 gtaaagtggt atttggcagt gtgcagtcgg tcgcccgtaa tattgatgcc tttcagggtg
    46861 aattttcgct gttgattgtc gatgaatgtc accgtattgg tgacgatgaa gagagccagt
    46921 atcagcaaat cctcactcac ctgacaaaag tgaatcccca cttacgcctg ctggggctga
    46981 ctgccacgcc ttttcgattg ggcaaaggtt ggatctacca gttccattat cacggcatgg
    47041 tacgcagcga tgagaaagcc cttttccgtg actgcattta tgagctgccc ctgcgttata
    47101 tgattaaaca cagctatctg acgccgccag aacgactgga tatgccagta gtgcaatatg
    47161 atttcagccg cttgcaggca cagagcaacg ggctgttcag cgaagccgat ctcaaccgtg
    47221 agctgaaaaa acagcaacgt attaccccgc acatcatcag ccagattatg gagttcgccg
    47281 aaaagcgtaa aggagtgatg attttcgccg cgacggtcga acacgcaaaa gagattgtgg
    47341 gattactgcc tgccgaagat gcagcactga ttactggcga cacccccggc gctgagcgcg
    47401 atgtgttaat tgaaaatttt aaagcccagc gttttcgcta tctggtcaac gtcgcggtac
    47461 tgaccaccgg atttgacgcc ccgcacgtcg atcttatcgc cattctgcgc cctaccgaat
    47521 cagtgagtct ttaccaacaa attgtcgggc gcggtctgcg tctcgctccg ggcaagactg
    47581 attgtttaat tcttgattat gcgggtaatc ctcacgatct ctacgcgccg gaagttggta
    47641 caccgaaagg caaaagtgac aacgttccgg tacaggtttt ctgccctgcc tgcggttttg
    47701 ccaacacctt ttgggggaaa acgaccgccg acgggacatt gattgaacac tttggtcgcc
    47761 gctgtcaggg atggtttgaa gatgacgacg gtcatcgcga acagtgtgac ttccgtttcc
    47821 gttttaaaaa ttgcccgcaa tgtaacgcag aaaatgatat tgccgctcgc cgctgccgcg
    47881 aatgtgacac ggtactggtt gatccggatg atatgttaaa agcggcgcta cgactgaaag
    47941 atgcgctggt attacgctgt agcggcatgt ctttgcagca tgggcacgac gagaaaggcg
    48001 aatggttgaa aatcacctat tacgatgaag acggtgcgga tgtgagtgag cgtttccgtc
    48061 tgcaaacacc tgcccagcgt accgccttcg agcagctttt tatccgcccg catacgcgca
    48121 caccgggcat cccgctgcgc tggatcaccg ccgccgatat cctcgcccag caagccttat
    48181 tgcgaccccc ggattttgtc gtcgcccgca tgaaaggcca gtactggcag gtgcgtgaaa
    48241 aagtgttcga ttacgaaggt cgttttcgtc gggcgcacga attacgcggt taatggtagt
    48301 tttgattgat gtataaggcg attgagtata gaatctcacc cgcttttgca tacgcaaagc
    48361 agatcactta cctgttgctg ggtcgcctgt agcaggatta atttaagaga gaaagaaatg
    48421 tttactatca acgcagaagt acgtaaagag cagggtaagg gtgcgagccg ccgcctgcgt
    48481 gccgctaaca agttcccggc aatcatctac ggtggcaaag aagcgccgct ggctatcgag
    48541 ctggatcacg acaaagtcat gaacatgcaa gctaaagctg aattctacag cgaagttctg
    48601 accatcgttg ttgacggtaa agaaatcaaa gttaaagctc aggacgtaca gcgtcacccg
    48661 tacaaaccga agctgcagca catcgacttc gttcgcgctt aattgctgaa taagttgtaa
    48721 aaaaccccgc tcccgcgggg ttttttgtat ctgcagatta tgcctgatta cggtattgct
    48781 attttttgca ggccagataa ggcgttcacg ccgcatctgg cattcagaac acaaaaccga
    48841 ttaattgccg ccagacgtcc ggcgctgcaa ttggtcgcgc aaattcggcg gcgtgccttt
    48901 aatggtcagg gtatcggtcg ccggctccca gaagatgcgt tcacctaaca gcatcgcatc
    48961 aaagttaata gtcaagccac cgccgctacc ggcaaacttc gtcaactgac gcagtgtgct
    49021 ccggtctgcc gggaagctct cttccagttc gtagcctttt tcagccgcaa actccgtgaa
    49081 gctcacttcg cttacgcctg ccagctcttt cgacagcgat tccagttcga tctcttcgcc
    49141 tgcctgcagc tgctcattac agtagctata aacctgctgg cgcacgttct gtcgctctgc
    49201 tttatccagt tgtgcttcgg cagtgaaatc atcaaccgct tgcaacagcc cacggttttg
    49261 tgctttcgtg ttcaatcctt cgctggcccc gaggaaatcc ataaagaaat ccgccacttt
    49321 gcgccctact cgccctttca ggaaagtgag atagcgggtg gactctggat tggtttccca
    49381 ttcggttaaa tcgatacgcg caacaatatc cgcatgattg atatcaagat aatgggttgg
    49441 gttgatgtcg agattttcgt taacacgcat actgctcagg ttgctcagaa ctgccaccag
    49501 cagatactcc accgccagat aacggtagtg gcaaaacagc acgaatccac cgtcagcaaa
    49561 aggatatttc gctaattcgt cacgcaggcg accagttgcc gcgcggctaa atgccagaaa
    49621 gtcctcttcg ccctgacgct gtaagcgcag cgtctgcgcc agttcactct cttcgctaaa
    49681 caagccgtaa gctttatttt tggcgctata gacccgatgc agttctgcca ccatctcaac
    49741 gacggtttct gtcggttcca gcaatgaatc gcgcaacacc agctcaaggt tctgctcatc
    49801 acgcttgata agctggtgca gagcaatctg gttgatatcc agactcatga taaactctcc
    49861 tttaagaccg ggcggtattc aaccaccgcc ggttaagcga cgcaataaaa gatggcggtc
    49921 attttacaga aggcagcgta acgagaatga tttaacgcat aaaaaagcag aaaaaaaacc
    49981 gttgctacgg taatatgttg ccctttcatc aactaactga tttcgattta tgccacaaat
    50041 ttcccgctac agtgatgaac aggttgaaca attgctcgca gagctgctca acgtactgga
    50101 aaaacataag gctccgaccg acctttccct gatggtgtta ggcaacatgg tcactaacct
    50161 tatcaacacc agcattgccc cggcccaacg ccaggcaatt gccaactctt ttgcccgcgc
    50221 cttacagtcc tctatcaacg aagacaaagc gcactaaggg aaacagataa caggttatgg
    50281 taactcatcg tcagcgctac cgtgaaaaag tctcccagat ggtcagttgg gggcactggt
    50341 ttgcactgtt caatattctg ctttcgctcg tcattggcag ccgttacctg tttatcgccg
    50401 actggccgac aacgcttgct ggtcgcattt attcctacgt aagcattatc ggccatttca
    50461 gcttcctggt gttcgccacc tacttgctga tcctcttccc gctgaccttt atcgtcggct
    50521 cccagaggct gatgaggttt ttgtccgtca ttctggcaac ggcgggaatg acgctattac
    50581 tgatcgatag cgaagtcttt actcgtttcc atctccatct taatcccatc gtctggcagt
    50641 tggttatcaa cccagacgaa aatgagatgg cgcgcgactg gcagctgatg ttcatcagcg
    50701 tgccggttat tttattgctt gaactggtgt ttgcgacgtg gagctggcaa aagctgcgca
    50761 gcctgacgcg tcgtcgacgc ttcgcgcgcc cgctggccgc attcttattt atcgccttta
    50821 tcgcctcgca tgtggtgtat atctgggccg atgccaactt ctatcgcccg atcaccatgc
    50881 agcgcgctaa cctgccgctt tcgtacccga tgacggcgcg acgttttctt gagaagcatg
    50941 gtctgcttga tgcgcaggag tatcaacgcc gtcttattga gcaaggtaat ccagacgccg
    51001 tttccgttca gtatccgtta agcgaactgc gctatcgcga tatgggcacc gggcagaatg
    51061 tgctgttgat tactgtcgat ggcctgaact actcacgctt cgagaagcag atgcctgcgc
    51121 tggcaggttt tgctgagcaa aatatttcgt tcacgcgcca tatgagctcc ggcaacacta
    51181 cagacaacgg catctttggc ctgttctatg gtatctcgcc gagctatatg gacggcattc
    51241 tgtcgacccg tacgcctgcg gcattaatta ctgcgcttaa tcagcaaggc tatcagctgg
    51301 ggttattctc atcagatggc tttaccagcc cgctgtatcg ccaggcattg ttgtcagatt
    51361 tctcgatgcc gagcgtacgc acccaatccg acgagcagac cgccacgcag tggatcaact
    51421 ggctgggcca ctacgcacaa gaagataacc gctggttctc gtgggtttct ttcaatggca
    51481 ctaacattga cgacagcaat cagcaggcat ttgcacggaa atatagccgg gcggcaggca
    51541 atgtcgatga ccagatcaac cgcgtgctca atgcactgcg tgattctggc aaactggaca
    51601 atacggttgt gattatcact gccggtcggg gtattccgct gagcgaagac gaagaaacct
    51661 ttgactggtc ccacggtcat ctgcaggtgc cattagtgat tcactggcca ggcacgccgg
    51721 cgcagcgtat taatgcgctg actgatcata ccgatctgat gacgacgctg atgcaacgcc
    51781 tgctacatgt cagcacacct gccagcgaat attcgcaagg tcaggatttg ttcaaccctc
    51841 aacgccgtca ttactgggtt actgcagcgg ataacgatac gctggcaatt accaccccga
    51901 aaaagacgct ggtgctgaac aataacggta aataccgcac ttacaactta cgtggtgaaa
    51961 gagtgaaaga tgaaaaacca cagttaagtt tgttattgca agtgctgaca gacgagaagc
    52021 gttttatcgc taactgatta attataaatc agttagcgaa atatcttact tgcaatcggt
    52081 gtggaaaacg gtagtattag cagccacgag tcggcacgta gcgcagcctg gtagcgcacc
    52141 gtcatggggt gtcgggggtc ggaggttcaa atcctctcgt gccgaccaaa aatcccaaga
    52201 aaaaaccaac ctttacggtt ggttttttta tatctgcaat taatttgata aacagaccgt
    52261 gacacatcac agcctgttta ttttctgtta tcagaacgtc cagaccactc ccgcctgagt
    52321 attccacggc gattctaccc ctgcgccatt gccatagctg actgacaaat gaccgcttaa
    52381 cgtcggggta aacgatgacc ttatcccagc ctgataaacg ccacgggttc cagccacatc
    52441 attattaaat ttgccatcgt cattaacttt cacatggtta gaatcggcgt attcctgacg
    52501 cacggcggct ttcagccagg gttccaacgt cgtaccgttt tgcaggtcca tgtgatagct
    52561 taccgccgtt cccgcttcag cgcgtaatat ccgggtattt cccacatccg cgcgcatgcc
    52621 gtttgataac gtgtagtcct gaccatctgt ggtaaagccg gtaaaggcga gatagggtct
    52681 aacactccac aatccgtcaa cccaacggaa cccgctctca acatgagcac ccgcgccgtt
    52741 actattgtaa tcgccaaacg ctgttgcccc attactcatc ttgccatgga tggtgttggc
    52801 aaaacggtca actttcacca ccccatcaac ataggcaccg ttctgatgct cccaaccggc
    52861 ataagccccc agggtgtagc tatcaatatt gcctttgccg ccgcgatcaa aaccaatatc
    52921 agaatgagag taatcaaaga acaagccgcg aattgtgctg ctttcttcac gggagaaacg
    52981 gctatcgata ccgagcgtca ggcccgtcaa tgtttgctca aagccagctc ccgcatcagt
    53041 ggtcacgttg ttgcgggtgt taattgccga actccacatc gccgtatcgt aattaacgcc
    53101 ttttacgcta ccaagacgct cacgcacggt gtccagttct gcatcaaata ccagcggttg
    53161 tgctgctgcc atgttcagca catcagtggt tgaaggggta atttgcgcgc gattctctgc
    53221 gaggctccag ctatggttgc cattatccag caaggtatat tcatacgtac cgatatcaac
    53281 aacgcctccg gcattgccca acgtgaatgc agcatcaccg ccgcccgtcg ttaccagtgt
    53341 aaggctatct cctgctgccg ggctggcccc cgtgtccgtc acgaatattt tgaaatcacc
    53401 tgttgcctga ccgatgacgt tgagctgatc gctctgatga ttagccatat cggtacgcat
    53461 ataaaattgc cgtttcccga cagggtattg gtggtcaacg taatgaaatt ttccgcactt
    53521 gccgtcacag atgaacgagt cactggctca gccatcataa tcacatttgc gccggacatc
    53581 gcaaggttgg ctaagttgag gcgagtgttt tggttcgtcg tagtgatatc ggcaatgatg
    53641 gtccatgcgc tattttcgag cgaaacgtcc gcttggctgg tatccacggc accatgcgtt
    53701 ctaaaagaag cgccctcaga aaccactacc ttacccacca acatagcggg tgcataattt
    53761 attttcggct tcatgacctc aagaatgcca tcattccctc tgactgacac gttaaccatt
    53821 gtgccagccc agacattagc gcggccattg ttgataacca tattttcaga gatggcttta
    53881 gaggaatacg tgatacttcc attctgatct gatcgaccga gatcatatac agcatttgcc
    53941 tgcactttcg taccagtatc ctttcccagc gattgcatag tggcatgctc atcaaggata
    54001 gtgtcaatgg cctgagtgtc ctccataaca atgagcccgg agccatcatc cagttcataa
    54061 ttttttgaca caccatcctt tatactaaat tggcctttac tgtttgtacc actcacttcc
    54121 agcgcattcg ttgagacaat taatttcccg cctgcttttt tatcaacgcc agttgcagtc
    54181 ccgccatcca taacctccag taatcctccc ttttcgatag cggcaaagac ggcaatccca
    54241 tctttttcaa caattaagcg cccgccttta atcgtgggat cattcgccgc g
//
LOCUS       DAZPAC010000004        49503 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZPAC010000004 DAZPAC010000000
VERSION     DAZPAC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.004"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            53..685
                     /gene="comR"
                     /locus_tag="ACHUPM_000165"
     CDS             53..685
                     /gene="comR"
                     /locus_tag="ACHUPM_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR0868068.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(747..1286)
                     /locus_tag="ACHUPM_000166"
     CDS             complement(747..1286)
                     /locus_tag="ACHUPM_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0868069.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1513..2817)
                     /gene="ndh"
                     /locus_tag="ACHUPM_000167"
     CDS             complement(1513..2817)
                     /gene="ndh"
                     /locus_tag="ACHUPM_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR0868070.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3225..3767)
                     /gene="ycfP"
                     /locus_tag="ACHUPM_000168"
     CDS             complement(3225..3767)
                     /gene="ycfP"
                     /locus_tag="ACHUPM_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR0868071.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3790..4815)
                     /gene="nagZ"
                     /locus_tag="ACHUPM_000169"
     CDS             complement(3790..4815)
                     /gene="nagZ"
                     /locus_tag="ACHUPM_000169"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR0868072.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4826..5650)
                     /gene="thiK"
                     /locus_tag="ACHUPM_000170"
     CDS             complement(4826..5650)
                     /gene="thiK"
                     /locus_tag="ACHUPM_000170"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR0868073.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5631..6272)
                     /gene="lpoB"
                     /locus_tag="ACHUPM_000171"
     CDS             complement(5631..6272)
                     /gene="lpoB"
                     /locus_tag="ACHUPM_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR0868074.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVKAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6286..6663)
                     /locus_tag="ACHUPM_000172"
     CDS             complement(6286..6663)
                     /locus_tag="ACHUPM_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR0868075.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6666..7025)
                     /gene="hinT"
                     /locus_tag="ACHUPM_000173"
     CDS             complement(6666..7025)
                     /gene="hinT"
                     /locus_tag="ACHUPM_000173"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR0868076.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7359..9548
                     /gene="fhuE"
                     /locus_tag="ACHUPM_000174"
     CDS             7359..9548
                     /gene="fhuE"
                     /locus_tag="ACHUPM_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFR0868077.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(9608..11041)
                     /gene="ptsG"
                     /locus_tag="ACHUPM_000175"
     CDS             complement(9608..11041)
                     /gene="ptsG"
                     /locus_tag="ACHUPM_000175"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR0868078.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11336..12133)
                     /locus_tag="ACHUPM_000176"
     CDS             complement(11336..12133)
                     /locus_tag="ACHUPM_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0868079.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12144..13148)
                     /gene="holB"
                     /locus_tag="ACHUPM_000177"
     CDS             complement(12144..13148)
                     /gene="holB"
                     /locus_tag="ACHUPM_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR0868080.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13145..13786)
                     /gene="tmk"
                     /locus_tag="ACHUPM_000178"
     CDS             complement(13145..13786)
                     /gene="tmk"
                     /locus_tag="ACHUPM_000178"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0868081.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13776..14798)
                     /gene="yceG"
                     /locus_tag="ACHUPM_000179"
     CDS             complement(13776..14798)
                     /gene="yceG"
                     /locus_tag="ACHUPM_000179"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR0868082.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14801..15610)
                     /gene="pabC"
                     /locus_tag="ACHUPM_000180"
     CDS             complement(14801..15610)
                     /gene="pabC"
                     /locus_tag="ACHUPM_000180"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR0868083.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15730..16971)
                     /gene="fabF"
                     /locus_tag="ACHUPM_000181"
     CDS             complement(15730..16971)
                     /gene="fabF"
                     /locus_tag="ACHUPM_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR0868084.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17059..17295)
                     /gene="acpP"
                     /locus_tag="ACHUPM_000182"
     CDS             complement(17059..17295)
                     /gene="acpP"
                     /locus_tag="ACHUPM_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR0868085.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17506..18240)
                     /gene="fabG"
                     /locus_tag="ACHUPM_000183"
     CDS             complement(17506..18240)
                     /gene="fabG"
                     /locus_tag="ACHUPM_000183"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR0868086.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18253..19182)
                     /gene="fabD"
                     /locus_tag="ACHUPM_000184"
     CDS             complement(18253..19182)
                     /gene="fabD"
                     /locus_tag="ACHUPM_000184"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR0868087.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19198..20151)
                     /gene="fabH"
                     /locus_tag="ACHUPM_000185"
     CDS             complement(19198..20151)
                     /gene="fabH"
                     /locus_tag="ACHUPM_000185"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR0868088.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20219..21289)
                     /gene="plsX"
                     /locus_tag="ACHUPM_000186"
     CDS             complement(20219..21289)
                     /gene="plsX"
                     /locus_tag="ACHUPM_000186"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR0868089.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21370..21543)
                     /gene="rpmF"
                     /locus_tag="ACHUPM_000187"
     CDS             complement(21370..21543)
                     /gene="rpmF"
                     /locus_tag="ACHUPM_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR0868090.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21595..22116)
                     /gene="yceD"
                     /locus_tag="ACHUPM_000188"
     CDS             complement(21595..22116)
                     /gene="yceD"
                     /locus_tag="ACHUPM_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR0868091.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22315..22899
                     /gene="yceF"
                     /locus_tag="ACHUPM_000189"
     CDS             22315..22899
                     /gene="yceF"
                     /locus_tag="ACHUPM_000189"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR0868092.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23011..23970)
                     /gene="rluC"
                     /locus_tag="ACHUPM_000190"
     CDS             complement(23011..23970)
                     /gene="rluC"
                     /locus_tag="ACHUPM_000190"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR0868093.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24543..27728
                     /gene="rne"
                     /locus_tag="ACHUPM_000191"
     CDS             24543..27728
                     /gene="rne"
                     /locus_tag="ACHUPM_000191"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR0868094.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27923..28876)
                     /gene="flgL"
                     /locus_tag="ACHUPM_000192"
     CDS             complement(27923..28876)
                     /gene="flgL"
                     /locus_tag="ACHUPM_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFR0868095.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28888..30531)
                     /gene="flgK"
                     /locus_tag="ACHUPM_000193"
     CDS             complement(28888..30531)
                     /gene="flgK"
                     /locus_tag="ACHUPM_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFR0868096.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30597..31538)
                     /gene="flgJ"
                     /locus_tag="ACHUPM_000194"
     CDS             complement(30597..31538)
                     /gene="flgJ"
                     /locus_tag="ACHUPM_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR0868097.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31538..32635)
                     /gene="flgI"
                     /locus_tag="ACHUPM_000195"
     CDS             complement(31538..32635)
                     /gene="flgI"
                     /locus_tag="ACHUPM_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR0868098.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32647..33345)
                     /gene="flgH"
                     /locus_tag="ACHUPM_000196"
     CDS             complement(32647..33345)
                     /gene="flgH"
                     /locus_tag="ACHUPM_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR0868099.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33398..34170)
                     /gene="flgG"
                     /locus_tag="ACHUPM_000197"
                     /pseudo
     CDS             complement(33398..34170)
                     /gene="flgG"
                     /locus_tag="ACHUPM_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(34342..35097)
                     /gene="flgF"
                     /locus_tag="ACHUPM_000198"
     CDS             complement(34342..35097)
                     /gene="flgF"
                     /locus_tag="ACHUPM_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFR0868100.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35117..36322)
                     /gene="flgE"
                     /locus_tag="ACHUPM_000199"
     CDS             complement(35117..36322)
                     /gene="flgE"
                     /locus_tag="ACHUPM_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR0868101.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36347..37042)
                     /gene="flgD"
                     /locus_tag="ACHUPM_000200"
     CDS             complement(36347..37042)
                     /gene="flgD"
                     /locus_tag="ACHUPM_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR0868102.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37054..37458)
                     /gene="flgC"
                     /locus_tag="ACHUPM_000201"
     CDS             complement(37054..37458)
                     /gene="flgC"
                     /locus_tag="ACHUPM_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFR0868103.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37462..37878)
                     /gene="flgB"
                     /locus_tag="ACHUPM_000202"
     CDS             complement(37462..37878)
                     /gene="flgB"
                     /locus_tag="ACHUPM_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR0868104.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38033..38692
                     /gene="flgA"
                     /locus_tag="ACHUPM_000203"
     CDS             38033..38692
                     /gene="flgA"
                     /locus_tag="ACHUPM_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR0868105.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            38768..39061
                     /gene="flgM"
                     /locus_tag="ACHUPM_000204"
     CDS             38768..39061
                     /gene="flgM"
                     /locus_tag="ACHUPM_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR0868106.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            39066..39482
                     /gene="flgN"
                     /locus_tag="ACHUPM_000205"
     CDS             39066..39482
                     /gene="flgN"
                     /locus_tag="ACHUPM_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR0868107.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39522..41057)
                     /gene="murJ"
                     /locus_tag="ACHUPM_000206"
     CDS             complement(39522..41057)
                     /gene="murJ"
                     /locus_tag="ACHUPM_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR0868108.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41167..42090)
                     /locus_tag="ACHUPM_000207"
     CDS             complement(41167..42090)
                     /locus_tag="ACHUPM_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0868109.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42092..42739)
                     /locus_tag="ACHUPM_000208"
     CDS             complement(42092..42739)
                     /locus_tag="ACHUPM_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFR0868110.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42750..43334)
                     /gene="rimJ"
                     /locus_tag="ACHUPM_000209"
     CDS             complement(42750..43334)
                     /gene="rimJ"
                     /locus_tag="ACHUPM_000209"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0868111.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43570..44778
                     /gene="mdtH"
                     /locus_tag="ACHUPM_000210"
     CDS             43570..44778
                     /gene="mdtH"
                     /locus_tag="ACHUPM_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR0868112.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            44842..45489
                     /gene="grxB"
                     /locus_tag="ACHUPM_000211"
     CDS             44842..45489
                     /gene="grxB"
                     /locus_tag="ACHUPM_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR0868113.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45623..46183
                     /locus_tag="ACHUPM_000212"
     CDS             45623..46183
                     /locus_tag="ACHUPM_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0868114.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46289..47335
                     /gene="pyrC"
                     /locus_tag="ACHUPM_000213"
     CDS             46289..47335
                     /gene="pyrC"
                     /locus_tag="ACHUPM_000213"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR0868115.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47409..47654
                     /gene="dinI"
                     /locus_tag="ACHUPM_000214"
     CDS             47409..47654
                     /gene="dinI"
                     /locus_tag="ACHUPM_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR0868116.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47944..48198
                     /gene="bssS"
                     /locus_tag="ACHUPM_000215"
     CDS             47944..48198
                     /gene="bssS"
                     /locus_tag="ACHUPM_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR0868117.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            48313..49431
                     /gene="solA"
                     /locus_tag="ACHUPM_000216"
     CDS             48313..49431
                     /gene="solA"
                     /locus_tag="ACHUPM_000216"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR0868118.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        12024 a        13338 c        12280 g        11861 t
ORIGIN      
        1 tgattatttt tataataacc tttcattcac agtcaccagg ttcaacggca tcatggcaac
       61 tgactcaaca caatgtgtaa aaaaaagccg tggccgccca aaagtgttcg acagggatgc
      121 cgcgcttgat aaggccatga aattgttctg gcaacacggt tatgaagcga cttctcttgc
      181 ggacctcgtc gaagcgaccg gagccaaagc gcccacgcta tacgcggaat ttaccaacaa
      241 agaggggtta tttcgtgccg ttctcgaccg ctatatcgat cgttttgccg ctaagcatga
      301 agcacagctg ttttgtgaag agaaaagcgt ggagtctgcg ctggctgact attttgctgc
      361 catcgccaac tgctttacca gcaaagacac cccggcaggc tgcttcatga tcaacaactg
      421 caccaccctc tccccagatt caggagatat cgccaatacg ttgaaatcac gccatgcgat
      481 gcaagagcgc actttgcagc agtttttatg tcaacgacaa gcgcgcgggg aaatcccgac
      541 ccactgtgac gtgacacatc tggcagaatt ccttaattgt attattcagg ggatgtcgat
      601 cagcgcacgc gaaggtgcat cgctggaaaa actgatgcag attgccggaa cgactttgcg
      661 tttatggccc gaactggtga aataagtcgc aaaaaaagcc cctcagcctg agcgaatgag
      721 gggccaaatt gcagagtaca gccttgttat actttgttat tcaaaatcag ttgcccgtta
      781 ctatctagcg ggatctgcgt acccggatcg cggtccatgc ggattttgcc ctgctgatcg
      841 ccaatcttat aggtcacatc ataaccgagc attttttctg acttgtcata caccgtttta
      901 caacgctgtt gcgtagtcgt gtaagtatcg ctttcctgga gagagccctg gatctggtta
      961 ccggcatatc caccacccag cgcccccaca acagtggcga catctttacc acgaccacca
     1021 ccaaactgat gccctatcac gccgccagca acagcgccga gcaccgaccc ggtaatgcga
     1081 ttttcatcct gcaccggtcg acgatgggtc actgtgacgt tgcgacactc ctgacgcggt
     1141 gttttaaccg tttccttgat tggggttgca gaaacaacct gagcgtattg cgggccccgt
     1201 tcaaacacgt tcagactggc cactgccgct acgcccagcg cagctgcgac accaatcccg
     1261 atacccgcca acattgattt attcacggga acacctcctt ctttgcagtt atgccaatca
     1321 tgcaagcgaa ggagaaaagc agctatcaga cagaagatga aaagcgcggg aatggctgga
     1381 aatacttaag gattcggaga agtctgaagg aatgcctgat gcgacgctta acgcgtctta
     1441 tcaggcctac cgctcgtacc atttgtccag ggcggatgag gcgtttatgc cacatccgcc
     1501 agtgtacgtc gattaatgca acttcaaacg cggacggata acgcggttaa tactccccac
     1561 cagcatcatt aatccggttt taaagtaacc atgcagcgca atctgatgca ttcggtatag
     1621 cgagatatat acaaagcgcg caattcgtcc ttcaatcatc attgagccgc gcgtcaggtt
     1681 acccatcagg ctaccgacag tggagaagtt cgacagcgat accagcgaac catgatcttt
     1741 atactgataa tttttcagcg gtttaccgtt catctgcgcc agaatatttt tcattgcgca
     1801 ggtcgccatc tggtgtgcag cctgagcacg cggcggaaca aagccccctt ccggacgcgg
     1861 gcatgacgcg cagtcgccaa tagcgtaaat gtctggatcg cgggtggttt gcagcgtcgg
     1921 ttccaccacc agctggttga tacggttggt ttcaagacca ccgatatctt tcaggaagtc
     1981 tggcgctttg atcccggctg cccacaccat cagatcagcc tcaatatatt caccatcttt
     2041 agtgtgcagg ccgccttcat cagcactggt gaccatggtt tgcgtcagca cgcgaacgcc
     2101 aagtttcgtt agctcgttgt gggccgcagc agagatacgt ggcggtaatg caggcaaaat
     2161 acgttctccc gcttctacca gcgttacgtt cagggcttcg ttggtcaggc ctttgtaacc
     2221 gtagctgtgc agttgcttga ccgcattgta caattcagcg gagagttcta cacccgtcgc
     2281 gccgccgccg acaatcgcaa tgttcacttt accattcgcg cccaggttgg cggagtattt
     2341 caggaacaga ttcagcatct cctggtggaa gcgacgcgcc tggtgcgggt tatcgaggaa
     2401 aatgcagttc tctttgacac ctggcgtatt gaaatcgtta gaggtgctac ccagcgccat
     2461 taccagggtg tcataggcga ttttacgttc cggaaccagc agttcacctt tctcatcgcg
     2521 cagttctgca atagtgattg ttttcgcttc acgatcaata tcaatgacgg aacccagctg
     2581 gaactggaaa ccatgattgc gcgcatgggc cagatagctc aacgcatcga cgccttcatc
     2641 aagcgagcca gtcgccactt cgtgcagcag cggtttccac agatggctgt ggttacgatc
     2701 gaccagcgta attttggctt ttttcttgcg tcccagctta tgccccagct gtgttgccat
     2761 ttccagccca ccagcaccgc cgccgacaat cacaattttt ttcaatggcg tagtcaacgt
     2821 gaccccctta aatttattaa ccaattgtta attaatggtt ataaacatag ctcttaatta
     2881 acaacaggtt acagcaatga aaatgttctt cgctattgag aataacatga atggtgcatt
     2941 ggtcatacca aaattgatgt taatcaagtt ttgttgctga aaagataggc gactttaccg
     3001 ggtgagatca aaaaacagag tttattgttt attatttgag caagtccgga agggtgatat
     3061 agggtgacgc gtatatcgag ggcgtttggt cgtcctgata agatgcgcag catcgcatca
     3121 ggcattgatg ctcgactgtc ggatgcggcg taaacgcctt atccgactta cagaattcgg
     3181 agcgcatcag gtaccggcaa ggttacctga tgcgacgagg gcatttaccc gagggttttg
     3241 aacgccttaa tgcgctgcaa atgcggggag atattcttga atttgtgcgt ctgctcttcg
     3301 tcccagacaa tctcgtaata atgatgcaac tcttcagatg tccgctggct gttaagcgct
     3361 tcatcattac gcgacaaaat caccaggcaa cgatcgcgat tcttctcgcg gaagttggtc
     3421 acacacttag tggcaatatc ggcatactct tccgggcgat caatcttgcc ttccatgttc
     3481 tcataaggga acaaattagg gttgaagatc acctggcgga tatcgcaaag aaaaccaatc
     3541 cgttccgccc agtatccgcc cagaccaacg ccgcaaatca gcggacgctc gtcgacgttc
     3601 agttgcaaca ttttgtccac ttctttgagc agatgctgca tatcatgttt cggatgccgc
     3661 gtactgtagc ttatcaagcg tacatccggg tcaataaact gcaattgtaa gactttctcg
     3721 tggttacccg gactgttaga gtcaaaaccg tgtaaataga taatcatcgc ttcctcacat
     3781 aagccagggt tagtgacctg ctttctcttc ctgccagcgt tcatgtaact gattcagacg
     3841 ggtgctgatc gccttccagc gagccgagtc catcagttcc tgtcgcgaaa atgaaccttt
     3901 atgatacaaa cgtgtaacac gttctgcctt gatcggtgac agattatcta acacgctgac
     3961 ggccccttta cgattattgc agaccaggat catatcgcaa cctgcgtcca gtgatgcctg
     4021 cccgcgttcg gcataactgc ccataatcgc ggcaccttcc atcgataaat cgtcagagaa
     4081 aattacaccg tcaaaacaca gttcctgacg caaaacggtt ttcagccagt aggaagaacc
     4141 gctcgccgga cgcggatcaa catcactgta gatcacatgc gcaggcataa tggcgtcgag
     4201 tttattttcg cggattaagg aactgaagac cgacatatct ttagcgcgaa tctccgcttg
     4261 tggacgtgga tcgcacggtg tttctttgtg tgagtctgcc gttactgcac cgtgtcctgg
     4321 gaagtgtttc ccggtcgttt tcattccggc ttcatgcata ccatcaataa accggctggc
     4381 aattgccagg gctttttgtg gatcggcatg ataagaacgc tcgccaatcg ccgcgctgat
     4441 atgcccgaca tccagcactg gcgcaaagct gatatcaata tccatagcga tcatttcgct
     4501 ggccatcagc caacccgcct cttgcgccag tttgccgccc tcttccattc ccaacagcgc
     4561 agcaaaggat tgtgctgccg gtaagcgggt aaaaccttcg cgaaaacgct gcacgcgtcc
     4621 accttcctga tctaccgcca ccaccagatg attgcgcgat gctgcgcgga tctggcgcac
     4681 cagttcacgt aactgggcag gatcatgata gttacgcgta aagagaatca gccctcccac
     4741 cagcggatgc gccagtattt cacgctcttc cgcgtccagt tcgtaacctt cgacatccaa
     4801 cattactgga cccacactgc tctccttatt gttttattaa tagctgccgc caggtgtcat
     4861 cggccagcct gataaattgt tgatcgccga tttgtcgcca gcggtactca aaccaccctg
     4921 ctttgagcat cagcagccag ggaaaccatc gcctgacctg acgccataat tgcgccggat
     4981 aaatcttcgc gcgagtggca tagtcattga ccaattgccg gtgctgttca gtattttcca
     5041 cccacaccgc cgccagttcc agcgcgatat caccatctcc ggcatactcc cagtcgatga
     5101 gttttaaccc tgacgcgcta tgcactaaat ttccggcgtg gacatccata tgcaatggac
     5161 ttaagcgtaa aggccgtggt tcccgcgctt tgcgcagacg ttttaacatt cgcagccaac
     5221 ccacagtccg ccgcgccgga tcgctttgct gccagtacag ttccagtaag ggcaacagcg
     5281 ttattcgcca gccaaaacgt ggttgttgat gaagataata cagcaagcct gccagttcgt
     5341 tggtatccgg caaatacgtt tttaccgcgc cgggcagata gtcgactacc atccagtcac
     5401 ggagatataa atgcggcttc ggtgcaatgc ttgcgggtag ttgtgataaa gcccgatact
     5461 ggcgcaagaa cgcggactgc ggcgcatcag gatcgtgcgg ctgacgcaca acaaaacgct
     5521 ggccctgatg ttcaatgaga aaactaccgc cactaagccc actattaaac gtcgtgacgg
     5581 gatgaaactg cgggaaaaag cgcgacagca attcgtcgcg cgtgatggga ttattgctgc
     5641 gaaacggcac ctttacctga ccagataatt tcgcccgtct gcaccagcat cagctgcatt
     5701 tgtagggtcg gagccttaac gttgccagag gcgctggagt acagcacgta atgagcgccg
     5761 acattgcggg caatgcctat ggctttacta cgggtaccta aactgtcctg cggcgacaaa
     5821 cctaactgtt gcttagccat cgacagctgc tgggcggaaa ccagggtaaa tttcccgtta
     5881 ttagccagcg catttcgcag cgtttcggtc gcttctgcgg cattcagcga accgttagta
     5941 cggttgttaa cgctatcaac cagcaggacg ctacccgcag tcaccccgtc agccccaagc
     6001 atcttactga ccatcggctg cattgcgcca ttccagtcat aatggcgaat atgcggcgca
     6061 ggcggtgccg tttgatcttc gtgctcaatt gggcctggct gctgcgggat cgtcggcacc
     6121 gagggcactg tggggacagg ctgttgtggc tcggctggtt gttccggcgc tggtttcact
     6181 tcttctaccg gtgcaggttc acgttgcccc acacacccgg cgagaaacat cgccagcgcg
     6241 gtaatcaagg cgtagcgact catttttgtc atcaagattc accccttaca aatatagata
     6301 aagtctgact ttgtgcgccc ccagaaaatt ggcgctgccg tacagcgtta ccgccgaatg
     6361 tgcgggaatg ctaacgctgc gtggcctttc cagaggatgc atctccagcc ctctggcgtc
     6421 ataccagtaa aaacgataat gaacggtaac gggttcttgc ctttcgttat aaagcgttga
     6481 ggatgctgaa ggttgaatat cagacgtcga aaggacgggc ttttctgcac tgatgcccgc
     6541 agccaataaa gatgactcca tcaccagcga ttgctcatca ttcaccggaa tttccggatg
     6601 tgaacgacag cccaccagca gtaacaacgc cagagacacc agcccaaagc atccttttct
     6661 catcgttaca gacctttatg cgccagcatt ggtcccagcg gacggccacc caacaagtgc
     6721 atatggatgt ggtaaacctc ttgtccgcca tggcggttgg tattcatgat cagacgatag
     6781 ccatcttcgg caataccttc ttgctcagca atttttgccg ctacggtgat catgcgtccc
     6841 agcgcctgct catgctcagc tgacacgtcg ttcacagtcg gaatgaggat attcggaatg
     6901 atcagaatat gcgttggcgc ttgcggcgaa atatcgcgaa acgccgttac cagatcatcc
     6961 tggtagacga tatcggaggg gatctcacga cgaataattt tgctgaatat agtttcttct
     7021 gccacgacat tttccttttt caaatagcca tgcgttgcgc catgctacgc cgcgccacac
     7081 tgcgagtata gagtatggtc gagttaaacc tgctctttca accttaactc acgatttctt
     7141 aagcaaaaaa tcgttttgct gccgtatata tcgccattat tcccatttct gcctgcgaat
     7201 gcgctgaaaa gacattctct gtttggttta cacgacaaat atgaatgcgc atatttctca
     7261 tttgcattta caaacaaaat tattcgcaca ctaaatataa ccgctcatca ccctcctccg
     7321 gcatgagcct gacgacaaca taaaccaaga gatttcaaat gctttcaaca caatttaaca
     7381 gggataatca atatcaagcc atcaccaaac cgtcactact tgccggttgc atagcactgg
     7441 cactattacc ttctgccgct tttgctgcac cagccactga agaaacggtg attgttgagg
     7501 gttcagccac agctccagat gatggcgaaa atgattacag cgtaacgtct acctctgcgg
     7561 gtaccaaaat gcagatgact caacttgata ttcctcagtc ggtcactatt gttagccagc
     7621 agcggatgga agatcagcag ttacaaacgc tgggcgaagt gatggaaaac acgctgggga
     7681 tcagcaaaag tcaggcggat tccgatcgtg ctctttatta ttcccgtgga ttccagatcg
     7741 ataactatat ggttgatggt atccccacct attttgaatc gcgctggaat ctgggcgacg
     7801 cactttctga tatggcactc tttgaacgcg tagaagtagt gcgtggcgcg acaggactca
     7861 tgaccgggac gggtaatcca tctgcggcaa ttaatatggt tcgaaaacac gcgaccagtc
     7921 gtgaatttaa aggcgatgtc tcggcggaat acggtagctg gaacaaagaa cggtatgtgg
     7981 cggatttaca aagcccactc accgaagacg gtaaaatccg cgcgcgaatt gtcggcggct
     8041 accagaataa cgactcatgg ctggaccgct acaacagtga aaagaccttc ttctcgggca
     8101 ttgtcgatgc tgatttaggc gatcttacga cgctttcagc cggttacgaa tatcagcgca
     8161 ttgatgttaa tagcccaacc tggggcggtt taccgcgctg gaatactgat ggcagcagca
     8221 acagttacga tcgtgcacgc agtaccgcac ctgactgggc gtacaacgat aaagagatca
     8281 acaaggtctt tatgaccctg aagcagcggt ttgctgatac ctggcaagcg acactgaatg
     8341 ccacccactc tgaagtcgaa tttgacagca aaatgatgta tgtcgatgcc tatgtaaaca
     8401 aagcggatgg tatgctggtt gggccataca gtaattatgg acctggcttt gattatgtcg
     8461 gcggcaccgg ttggaacagt ggcaaacgta aagttgatgc gctggatttg ttcgctgacg
     8521 gtagttatga attgtttggt cgtcagcaca atctaatgtt tggtggcagt tacagcaaac
     8581 aaaacaatcg ttacttcagt tcatgggcca acatcttccc ggatgaaatt ggcagtttct
     8641 acaactttaa tggcaatttc ccacaaaccg actggtcacc acagagcctg gcacaggacg
     8701 ataccacaca tatgaaatcg ttatatgctg ctactcgagt cacccttgcc gatccgctgc
     8761 atctgatcct cggcgcacgt tataccaact ggcgggttga tacgctgact tacagcatgg
     8821 agaaaaacca caccacgcct tacgctggtc tggtgtttga catcaatgac aactggtcga
     8881 cctacgccag ctatacctct attttccagc cgcaaaatga tcgtgacagt tcaggcaaat
     8941 atctggctcc aatcaccggt aacaactacg agctgggtct gaaatcggac tggatgaata
     9001 gccgtctgac caccacgtta gccatcttcc gtattgagca ggataatgtc gctcagtcca
     9061 ccggtacacc tatccccggc agcaacggcg aaaccgccta taaagcggtg gatgggacag
     9121 tcagtaaagg ggtggaattt gaactcaacg gcgcaattac cgacaactgg cagctgacat
     9181 ttggcgcaac gcgctatatt gcagaggata acgaaggaaa cgccgttaat cctaatctgc
     9241 cacgcaccac ggttaaaatg ttcaccagct atcggttgcc tgtcatgcca gagttgacag
     9301 tcggcggtgg tgttaactgg caaaatcgcg tgtataccga caccgtgaca ccgtatggca
     9361 ccttccgcgc cgagcaaggt agctacgcgc tggtggatct cttcacccgc taccaggtga
     9421 cgaaaaactt ctcgttacag gggaacgtca ataacctgtt cgacaaaacc tacgatacca
     9481 acatggaagg ttctatcgtc tacggcgcac cgcgtaattt cagcattacc ggcacgtatc
     9541 aattctgaat aacacctgta aaaaaggcag ccatctggcg gccttagtct ccccaacgtc
     9601 ttacggatta gtggttacgg atgtactcat ccatctcggt tttcaggtta tcggatttag
     9661 taccgaaaat cgcctgaaca ccagaaccag caaccactac gcccgctgcg cccagtttct
     9721 tcaggccagc ctgatccact ttagacacat cagcaacgct gacgcgcaga cgggtaatac
     9781 atgcgtcgag gttagtaatg ttttctttac caccaaatgc agcaaccaga gccggtgcca
     9841 tttcgctggt acctgttgct tttgcatctt cagtcgcgtc ttcacgaccc ggcgttttca
     9901 gatccagtgc tttaatcagc acgcggaaga tggtgtagta aacaatcgca taaccgatac
     9961 cgacgatcgg gaacagccac agtttgctgc tgttaccaga cagaacgata aagtcgatca
    10021 gaccgtgcga gaacgacgta ccgtcacgca tccccagaag aatacagatt gggaatgcca
    10081 ggcctgccag aatcgcgtgg atgatgtaca ggatcggcgc aacgaacatg aaggagaact
    10141 cgatcggctc ggtgataccg gtcaggaacg aggtcagcgc cgcggagatc ataataccgc
    10201 ccactttcgc gcggttttct ggtttagcag agtgccagat agcaattgcg gcagctggca
    10261 gaccgtacat tttgaacagg aagccaccag acagtttacc cgcagtcggg tcacccgcca
    10321 tataacgcgg aatgtcgccg tggaaaacct gacctgctgc gttggtgtat tcaccaatct
    10381 gcatctggaa aggtacgttc cagatgtggt gcagaccaaa cggcaccagg caacgttcga
    10441 tgaaaccgta aatgccaaac gcaactaccg ggttctggta agcagcccac tgagagaagg
    10501 tctggattgc agaaccaatc ggcggccaaa tgaaggacag cacaacgcca gtaaagatgg
    10561 cagccaggcc agaaatgatc ggcacaaagc gtttaccggc aaagaagcca agatactcag
    10621 gcagcttaat acggtagaaa cggttaaaca tgtacgctgc gatcgcaccg gagataatcc
    10681 ctccgagtac gccagtatcc gccaggtgtt tagaggcgat ttcttcagca ggtaaatgca
    10741 gtaccagtgg cgcaaccacg gccatggttt taaccatgat gccataggca acaactgcgg
    10801 ccagcgcgga tacgccatcg ttattggtaa agccgagggc gacaccgatc gcaaaaatca
    10861 gtggcatgtt tgcaaagacg gaaccgcctg cttctgccat aacatgcgat acaacggcgg
    10921 gcagccagct gaaattcgcg gaaccgacgc ccagcagaat acctgcgata ggcagtacgg
    10981 ataccggcag catcagcgat ttaccgacct tttgcaggtt agcaaatgca ttcttaaaca
    11041 taattgagag tgctcctgag tatgggtgct ttttttacgt tctcacgcgt ggcaaggggg
    11101 gagagcctcg ccgtgtacag ggcatctaag cgccctttat ttattacaca gagtaaaata
    11161 attcagtgcc gatttgtttg acggctatca catttcaact tcagatcatt ggagaagttg
    11221 cacattttga caaaacatat gtcacaatat ttcaaacgtg gttattcacc gcccaatttt
    11281 cggcggtgaa ctttactcgt tttagccatt aattacgagc tttaaaaaaa ctcattcaac
    11341 ggatggattg aaggcgggaa gcgtcgatgt gaaacagacg ggcgaagtta tcggtggtta
    11401 cctgcgccag ttcttcaacg gcaacacctt tcaacacagc catgtattct gcaacgtcac
    11461 gaaccatcgc aggttgattc tcttttcctc gatgcggtac cggcgcaagg taaggtgagt
    11521 cagtttccac cagtaaccga tccaggggga cataacgcgc agcatctcga agttgctccg
    11581 cattacggaa ggtcacaatg ccggaaaagg agatgtaaaa tccgagatcc agtaatttac
    11641 ccgccgtttc tctgtcctct gtaaaacagt gtagtacgcc accgcaatcc gtcacttttt
    11701 cttcgcgcag aattgccagc gtatcggcgc gggcgtcacg ggtatggacg ataaccggct
    11761 tgttcagttc acgaccaatc tggatatgat ggatgaagga ctcttgctga cgaactttag
    11821 tttccggtgt gtaataataa tccagcccgg tttcacccag cgctacaaca ccctcttctg
    11881 ccgccagacg gcgtaaatct tctacatcgt agggatcatt ctggttgagt ggatgcacgc
    11941 cacaagaaaa tacgacgttg tcacgttcgc ctaccagatc ccgcatatgt aaataacccg
    12001 gtaatgttgt ggcgactgcc agacaaaatt tcacatcgcg tgcggcggct ttcgccagaa
    12061 cgtcatccac gtccttatgc aaagattcat aatccagacc atcgagatgg cagtgtgagt
    12121 cgactaaaaa catgatgtct ctcttaaaga tgaggaaccg gtagcacaac gcccggttgc
    12181 aggtaatgct caatacgcag taaaagatcg gtgatgagaa gctcgcggtt gatgcctgtg
    12241 acagacatta actgttcacg aatgtggcaa acatccccca gtatagcctg caggcgcgag
    12301 ggagaaagat ggtttgccag ttcgacgaca aggcccggca catcaacatt ggtcacctgc
    12361 gcagcaccat gatggcgttt tagcgcatcc atcagcaacg ttgccagcca gtgtaaacgc
    12421 gccggagctt gttcatgatt aagggccgcc agcagcgaat accagtcgcc cgatggcacg
    12481 ctatatgcca atgcctgaca caatgtttca cgagcctgcc agttatctcc ctgaaacaac
    12541 gccagtgccg cgccaggcga accggcgctt aagcgcaatg cggcaagtaa tgcatcctgt
    12601 gacattgtca cttcgcgtga aagccaggtc acggcgtact gttccggcgg cggcgcaagg
    12661 taatgtaacc gacaacgact acgtaatgtt gccagtaaac gttcaggctc gcgggtagcc
    12721 aggaaaaacc aggtttctgc tggtggctct tcaagcgttt tcagcaatgc gttagccgcg
    12781 gcgtcggtca gtaaggcagc atcggttacc cagacaactt tcgcaccacc taggcgtgcg
    12841 tgctcattca gcttttcggt gacctcacgt actgcatcaa tgcccagcgt atttttccct
    12901 ttttcgggag ccagggtgta gtaatcggga tgcgtgccag cctgcatcaa ctgacatcca
    12961 cgacagtgac cgcaactttt gtggccctgc ggttgttggc agagtaaata acggctcagg
    13021 gcgtagatta aagcatcatc gcccatgccc ggtaacgcct gaatgagtag cgcatggtga
    13081 cctcttccgg cctgatagct ggctaccagt ttttcgaaat caggtcgtaa ccatggatac
    13141 catctcatgc gtccaactcc ttcacccagt gggtcacggt agtgcggatt gcatccatca
    13201 cggcctccag cggctgggtg gcatcaatgg tatgaatact tttatcttgt gcagccagtt
    13261 ccagatagcg ggcgcgggtg cgattaaaga aatcgaaaga ctcttgctca atacgatcca
    13321 gctcgccgcg cgcacgcgcg cgttttaagc caacttccgg ggtaacatcg agatagagcg
    13381 ttaagtcggg gcgaaaatcc ccgagaacag catcacgcag tgttgccagc atatgttggt
    13441 caataccacg tcctccgccc tgatacgcct gagtggagag atcgtggcga tcgccaatca
    13501 cccaggtgcc gttagccaga gctggtttga tgaccgtttc taccagttga acgcgcgcgg
    13561 cataaaacat cagaacttcg gctttatcgg taatgacttc atcgcctacc gatttgatat
    13621 ccagcaccag gcttcttaac ttttcggcaa gttgcgtacc gccaggttcc cgagtgaaaa
    13681 ccatgtcgcg gatacccagt tgctcaagcg tctcaaccac cacattacgc gcggtagttt
    13741 tgcctgcgcc ttccagcccc tcaatgacga tatacttact gcgcattttt ttccttaagc
    13801 actttcagat aatcctgcac agacttgtta tggctggcaa gattggtatt aaacgtgtga
    13861 ccacctttac catcggccac aaaatagaga tacggcgttt ttgccggatg cgcagcagcc
    13921 ttcagcgaat ccgcccccgg cgtagctatc gcacccggcg gcaaaccggt aatggtatag
    13981 gtgttatacg ctgtcggcgt ttccaggtct gcacgagaaa gtttgccatt ataacgctct
    14041 cccatcccgt aaatcacggt tgggtcggtc tgcaggcgca taccaatgcg taaacggttg
    14101 ataaataccg aggcaacctg atcgcgttca ctggcaacgg cggtttcttt ttcgataatt
    14161 gatgccatcg tcaccagctg atttttatct ttataaggca gaccgtccgc acgcccttcc
    14221 caggcgctat cgaccgcttt caccattttc ttgtgcgctc gcttgagtaa cgcgacatcg
    14281 gtggtattgg cggtatacat ccaggtgtct ggccagaacc aaccttcaat ccactccggg
    14341 ttttccagtt caagtgcctg cgctacggtg gcgtacttat cgtcactcag cgtgtgcttg
    14401 atatacggag cctcacgcaa ttgcttgagg tagtcgctca gacgcatccc ttctaccagt
    14461 cgcagcggga actgtgcttc tttaccgctt tccagcaatt tcagcatctc gcgcacggtc
    14521 atctgcggtg taaagcggta agtcccggct ttaaagtgag aaagatccgg ttcgatacgc
    14581 agcaaccatt gaaaaacccg tgggcgattg atgatcttat cggcataaag ctgttcacca
    14641 agcgccagac gtccggtccc tggcttcagg gtaaatatcg tctcttcttt gataagcaat
    14701 ttgctgtcgg caagatggcg aaccttccag acgcccacac cagcggcgat acccagtacc
    14761 accagcaata acaagataat caataacact tttttcatga ctaattcggg cgctcacaaa
    14821 gtggggctaa atattcatat aacgttgctg acgaaaagga gacatcgcca caggcacgta
    14881 cgggcatcac tggcattaac gcattacaaa taaccatctc atctgcctgc aacgcctctt
    14941 ccagagaggc ttgcacttcg acaagctgat aagaggattg tgccagcaaa cggatacaga
    15001 attgtcgcat aatgccgtta acacctgcct gatccagacg cggcgtataa actacattgc
    15061 ccttccgcca gaacaaatta gccgcacagc attccgtaac ccacccttcg ctgtcaagga
    15121 ccagcgcctc atcagcgttt gtctgctcaa gatgagagcg gatcaatact tgctcaagcc
    15181 gattaagatg tttaatacct gcaagatgag gattgcgccc cagccgcacc gggcttagcg
    15241 ccaacgtcat cccttcgtta cgcaaacggt cgtaatgtgc aggataaacc gtaacggaga
    15301 gaatccgcgt tgccggtcca ctgttcaatg tgctgtaccc tcgcccgcca ctaccgcgac
    15361 tgatcacgac tttcagcaca ccattttgct gttctgctgc cagcgttttc atctcttgtt
    15421 caagctgagg ccagaagtca cagaaaatca tcaaccgctg acaagcatcc tgtagtcgct
    15481 ggatatgcgc cgataacaaa ctgactttac cgtcgataac tctggcggtg gtaaaacaac
    15541 catcaccaaa ctgcgttgcc cgatcgctta ctgccagcga ttcctgctta tgaccgttaa
    15601 ttaagaacat accggctcct tatcagagaa ttgttagtgt ggcagcatgt tcactacgga
    15661 acaagtcgga ataaaagcta agaaaaaagg cccgcaagcg gaccttttat atgggtggga
    15721 aatgacaact tagatctttt taaagatcaa agaaccatta gtgccaccga agccgaagga
    15781 gttacacagc gtgtattcca ttccgctgac ctgacgtgct tcatgcggta cgaaatccag
    15841 atcgcaacct tcatccgggt tatccaggtt gatggttggc ggaacagcct gatcgcgcag
    15901 cgccaggatg gagtagatag attctactgc acccgccgca cctaacaggt gaccggtcat
    15961 agatttcgtg gagcttacca acacacggct tgcagcttca ccgaagatgg ttttcaccgc
    16021 ctgcgcttca gctttatcgc cagccggcgt agaagtaccg tgcgcgttaa cgtagccaat
    16081 ctgactcgct tcaatgcctg catcacgcag agcatttgcc atcgccagag ctgcgcctgc
    16141 gccattttct ggcggtgacg tcatatgata agcatcgctg ctcataccaa agccgacgag
    16201 ttcagcgtaa attttcgcac cgcgtttttt cgcgtgttcg tactcttcaa gtaccagcat
    16261 accggcacca tcgcccagta cgaagccatc acgctcttta tcccacgggc ggctcgccgc
    16321 ttgcgggtta tcattgcggg tagataatgc acgtgccgcg ccaaaaccac caacacccag
    16381 cggcgtactg gctttctctg cgccacctgc aaccatcacg tcagcatcgc catacgcgat
    16441 aatacgcgca gcatggccaa tgttgtgcac gccggaagta caggcggtcg cgatagagat
    16501 gcttgggcca cgcaggccat acatgatagt cagatgacct gccaccatgt tcacaatcgt
    16561 tgacggaacg aagaatgggc tgatcttacg tggaccaccg ttcatcagag atgtgtggtt
    16621 ttcttcgatc agtccgaggc cgccaatccc ggagccaatt gcggcaccaa tgcgggttgc
    16681 gttctcttcc gttatatcaa ggccagaatc ctgcatggcc tgaacgccag cgacaattcc
    16741 atattgaatg aaggcatcca tcttgcgctg ttctttgcgc gagataatgt cctcacagtt
    16801 aaaatccttt actaagccag caaatttcgt tgcataggcg ctagtatcga aatggtcgat
    16861 taggctgatg ccactctgac cggcaagcag agctttccag gtagactcta cggtattgcc
    16921 gacaggagac aacatgccca gtccggtcac aactacacga cgcttagaca cgtttgtcct
    16981 ccagggaggg aaaaaatgat tctagtggga caaaaagata aaactcaggc ggtcgaacga
    17041 ccgcctggag atgttcactt acgcctggtg gccgttgatg taatcaatgg cagcctgaac
    17101 ggtggtgatt ttctcagctt cttcgtccgg aatctcagta tcaaactctt cttccagagc
    17161 cattaccagc tcaacggtgt caagagaatc cgcgcccagg tcttcaacga aagaagcatt
    17221 gttggtaact tcttcctgct taacgcccag ctgttcgccg ataattttct taacgcgttc
    17281 ttcgatagtg ctcatactct taaatttcct atcaaaactc gctttcgcga tggttttcgt
    17341 agtgtataaa atgttgaaaa atttgcaact aaatcccggc aggtcttacc acgattttac
    17401 gttattttga ggccttaccc cccaataacg caaatcattt tgcactaatt attactcata
    17461 accacgcaga ctgcgcgcaa catgagcatt ttgtgcaaat cgcggtcaga ccatgtacat
    17521 cccgccgttc acatgcaaag tttcacccgt gatgtaagct gcttcgtcgg atgccaggaa
    17581 tgcaaccgcg ttggcgattt cctgtgcgcc gccgaggcga cccgcaggaa cctgcgccag
    17641 gatacccgca cgctggtcat cgctcagcgc acgtgtcatg tccgtttcaa taaagcccgg
    17701 agcaacaacg tttacagtaa taccgcgtga cgcaacttcg cgcgccagtg atttactgaa
    17761 gccgatcaag cccgctttcg ccgcagcgta gttggcctga ccgccatttc ccatggtacc
    17821 aaccacagaa ccgatagtga taatacgacc atgacgcttt ttcatcatag cgcgcattac
    17881 cgcttttgac agacggaaaa cagatgaaag gttggtttcg ataatatcgt tccactcttc
    17941 atctttcatt cgcattaaca ggttatcacg agtgataccg gcattattga ccaggatatc
    18001 cacttcacca aattctgcgc gaattttttc cagaacagat tcgatagatg ccgggtcggt
    18061 cacattcaac atcagacctt tgccgttggc acctaaataa tcgctgatcg cctgagcgcc
    18121 attttcactg gtcgcagtgc caataacttt cgcgccacgg gctgcgagcg tttcagcaat
    18181 tgcgcggcca attccgcggc ttgcaccggt taccagtgcg atttttcctt caaaattcat
    18241 gattttcctc ttttaaagct caagcgccgc tgccatcgct gaaggttcgt tcagcgccga
    18301 ggcggtcagg gtgtcgacaa tgcgtttcgt caggccagta agcaccttgc ccgggccgac
    18361 ttcatagaga tgttctacgc cttgcgctgc catgtactca acagacttcg tccactgaac
    18421 cgggttatac aactgacgta ccagtgcgtc acggatggca tcaccattgg tttcgcattt
    18481 cacatcaacg ttattcacaa cagaaactgt tggtgcgtta aaggtgattt tcgctaattc
    18541 tactgccagt ttgtcggctg ctggtttcat cagcgcacag tgagacggta cgctcactgg
    18601 taacggcaga gcacgttttg cgcccgcagc tttacaggca gcgccagcac gctcaaccgc
    18661 ttctttatga ccggcaataa ccacctgtcc cggagagtta aagtttaccg gagaaacgac
    18721 ctgacctgct gcagcttctt cacacgcttt cgcaatagac gcatcatcca gaccgatgat
    18781 tgccgccata gcgcccgtac cttccggtac ggcttcttgc atgagcttgc cgcgcatctc
    18841 aaccagacgc accgcatcag cgaaatcaat cacaccagcg caaaccagcg cggaatattc
    18901 ccccaggctg tgaccggcca tcattgccgg tgctttaccg ccctgctgct gccatacgcg
    18961 gtacagcgca acagaggcag tcaacagcgc tggctgggtt tgccaggttt tattcagttc
    19021 ttcagctggc ccctgctggg tcagcgccca caggtcgtag cccagcgccg cagaagcttc
    19081 agcaaacgtt tcttcgacaa ttggatagct cgccgccata tcagccagca ttccaacggt
    19141 ttgagaaccc tgtccaggga acacaaatgc aaattgcgtc atgttttaat ccttatccta
    19201 gaaacgaacc agcgcggagc cccaggtgaa tccaccgcca aaggcttcaa gcagaaccaa
    19261 ctgccccggc ttaatgcgcc cgtcgcgtac agcttcatcc agcgcgcacg ggacagaggc
    19321 cgcagaggta ttaccgtggc gatccagcgt caccacgaca ttgtccatag acataccgag
    19381 ttttttcgcc gttgcactga taatacgcag gttagcctga tgcggaacca gccagtccag
    19441 ttgagaacgg tcaagattat ttgccgccag cgtctcatca acgatgtgcg ccagttccgt
    19501 taccgcaacc ttgaagactt cgttgcccgc catcgtcaga tgaattgaat tctctggatt
    19561 cacacggtca gcattaggca gcgtcagcaa ctcaccatag ctaccgtcgg catgcagatg
    19621 ggtggagatg attcccggct cttcagaggc agccagcacc gcagcgcccg cgccatcgcc
    19681 aaaaataata atagtcccac gatcggttgg atcgcaggtg cgcgccagta catcggaacc
    19741 gacgaccaga gcatacttca ccgccccaga tttcacgtat tgatcggcta cgcttaatgc
    19801 ataggtgaaa cctgcgcagg ctgctgcaac gtcaaatgcc gggcaacctt taatgcccag
    19861 catgctttga atctgacaag ctgcgctcgg gaaagcgtgc gtagcagaag tcgttgccac
    19921 aacgatcagg ccaatctggt ctttctcaat gcccgccatc tcaattgcgc gtgtcgccgc
    19981 ttcaaagccc atggttgaaa cggtttcgtt tggcgcggca atgtggcgtt cgcggatacc
    20041 ggtacgagtg acaatccact cgtcagaggt gtccaccatt ttttccaaat cggcgtttgt
    20101 ccgcacttgt tcgggcagat agctgccagt accaataatc ttcgtataca tgtacgctca
    20161 gtcacttttc ggttatatac cgtcacttgc aaactgcgag ttcgctggca gcgtcctgct
    20221 accgcagagt tccgcttttg ccaccgtcca gcagctcaaa accagctggg tatacagatt
    20281 ccaggcgagc ggcaattcgc tgaggaactt gtcgctgcac cgcctgcact gcctgttcaa
    20341 tcgcgaccgc aaaagctcgc tgattggctg caccatgact ttttatcacc gtgccgcgca
    20401 atcctaacag acaggcgccg ttatactggt cggggttgag gtgactgaat cgcctcgtca
    20461 ggctcttttg tagccaacgc tttaataaca gtagccacca cgaccgtttt ttcccttcac
    20521 cctgagattt cagcagagaa aggaacatcc tgacaacacc ttccatcgtc tttaatgtga
    20581 catttcctgt aaagccgtca caaaccagca catctgtctt gccagttaac aactcattgg
    20641 cttcaagata gccgatataa ttgatagaag ggattgtttt aagcaccgct gaggcatccc
    20701 gaatactgtc gagacccttt acttcttctt caccaatatt gagcaacgcc acgcgaggat
    20761 tgggaatttc caccacctct tcagccagaa ctgagcccat aatggcaaat tgcaccaaca
    20821 ttgtactgtc acaatcgacg ttggccccta agtcaaggac caccgttttg cccttttgct
    20881 gatgtggtaa taccgtcacc agcgccggac gctcaatccc ctccaggggc ttgagtaata
    20941 attttgccag ccccatcagt gccccggtat taccggcact gacacaggct tgcgctcgac
    21001 cttctttcac cagctccagg gccacgcgca ttgaactccc acgactggcg cggatagctt
    21061 gcgaaggccg ggcatcactg gcgataactg actgcgcagg aataatctgc agacgcgaac
    21121 gttgttcaaa gtcagcttta gcaagtaatg gcgtgatggc gtcgggattg ccgactaaaa
    21181 gaagagtgag ttgcgaatta gaattcagtg cctgcaatgc tgcaggcact gtcacggaag
    21241 ggccaaaatc ccctcccatg acatctaacg ccagggttag acgtgtcaag gtatcgtcgc
    21301 cgcccggttt ggtctttccc cagttgcctg gggaaaatcc tcactaagct tcatcacgca
    21361 gacgcgtgat tacttagcga tgaccttgcg gccgcggtag taaccgtcgg cagtgatgtg
    21421 gtgacgcagg tgtttttcac cagaagtttt gtctacagac aggctggtga ctgcggtcag
    21481 cgcgtcatgg gaacgacgca tgccacgttt ggaacgggtt ggtttattct gttgtacggc
    21541 catggacctt actcctcaat tacggtttat ccccgatcca acggggagca ccaattactt
    21601 acgctttaag ctggctaata cggcaaatgg gtttggcttt tgcacttctt caggcagttc
    21661 accaaagacc atgtccgctt cggacacttc acagtgttca gaatcatgca ccggaactac
    21721 cggcaaggcg aggatgattt catcttcaac cattgcaagc agatcgattt caccgaattc
    21781 gttaacctca atcggttcat acgcttccgg cagtgcttca gcctgttcgt ctgaacgcac
    21841 aggactaaaa caatacgttg tgtagacctg atgagtaaac ggcttcccgc aacgctgaca
    21901 ctcgagcgtt accgtcacct tcgcatcgcc gtttaacact gcgagacgtt ggttatcgat
    21961 agcgaacgac atggagcatt ccacatcact gtccacactg actacggatt cggcgacgcg
    22021 ctcaacctga tcaggggtat agataccctg gtaatcaagg cgtttttgag ccgtacgaac
    22081 cggatcgaga gtcaggggta attttacctt ttgcataggg cgcgcatatt aactttgtaa
    22141 cgtcatagag tcaaagaaaa aggcagcctg tggttgcctt ttgccaataa ttcgcacaca
    22201 ttgcgggtta ctgctttatt ttcgttcagt gctgataacg aacatcaacc tgcaaaacga
    22261 agcgcatagt ttaaaatggc tatcatcaat acgctatatc tgatggaaaa aaatatgcct
    22321 aaacttattt tagcctccac ctcgccatgg cgtcgcgctc ttctggaaaa actgcaaatc
    22381 tcttttgaat gtgcagcacc agaggtcgac gaaaccccac gcagcgacga atcaccgcga
    22441 cagttggtgc ttcgactggc acaagaaaaa gcgcaatctc tggcgtcacg ttatccggat
    22501 catttaatta ttggttcaga ccaggtatgt gttcttgatg gcgaaatcac cggtaaaccg
    22561 ttaacggaag aaaatgcccg tctgcaatta cgcaaagcca gcggcaatat cgtcaccttc
    22621 tatactggac tggcgttgtt taattcggcg aatgggcatc tacaaacaga agtggagcct
    22681 tttgacgtcc atttccgtca cctgagcgag gcggagattg ataattacgt gcgtaaggag
    22741 catcccctgc actgcgcggg tagctttaag agtgaaggat ttggcattac gctgtttgag
    22801 cgtttagagg gacgtgaccc taacacgctg gttggtttac cgctaatcgc gctgtgtcag
    22861 atgttacgtc gggaagggaa aaacccgctg atgggatagt tgtttgcgct ggatgcggtt
    22921 acgtcgcatc cggcaatcgg tgcaggcggc ctgatgcggc gtaaacgcct tatcaggcca
    22981 accgcccatt atcaggctta tatgcttgta ttagcgcgcg ttacgcagct tttgcaaaca
    23041 acgcttcaaa ccttcatcca tcggcgcttc gatacgcatc acctcaccgg tccccggatg
    23101 ggtaaacttc aacgctgcgg cgtgcaggaa cagacgattt aatcccgtgc ctgcttcagt
    23161 gagctgtctg tcaaattcac ggtcaccgta gcgatcgtca aaggcaatcg gatgacccgc
    23221 atactgtgta tgcacacgga tctgatgagt acgtccggtt accgggctac aacgcaccag
    23281 ggtggcaaat gcatagcgtt cttccacttt aaagcgtgtt tccgacggtt tgccttcctg
    23341 actgacacgc acgatacgtt cgccgctttg cagaatattt ttcaataacg gcgcttgaac
    23401 gctcttcaca tgcgactgcc actgaccgcg caccagcgcc aggtaatctt tttgcattcc
    23461 tttttcacgt aattgctcat gcagagaacg caacgccgag cgttttttcg ctatcagcaa
    23521 cacacctgaa gtgtcccggt cgagacgatg aaccagttca aggaaccgcg cttccgggcg
    23581 caacgcccgc aaaccttcga taacgccgaa gcttaaacca ctgccgccat gtaccgccgt
    23641 accggaaggt ttattcagca ccaggatatg atcatcttca tataagatga cgtccgccag
    23701 cgccgccacc ttttgcagat gtggcgaaac cgcctcttct tcccgctcag caacgcgaac
    23761 cggtgggata cgcacctcat cacccgcttc gagtttatat tcaggcttaa tacgtttttt
    23821 gttcacccgc acttcgcctt tacgcaaaat acggtaaatc atactttttg gtacgccttt
    23881 caattgggta cgcaaaaagt tatcgatacg ttgccccgct tcgtcagcgg tgatagcaac
    23941 aatttttacg gatggagtct ctgttttcat ggtgggcgat tctaattagc caacaggatt
    24001 cgcgccactc atttttctat gcttatattt agttttgcac cttattactt cactacgtga
    24061 tcactttatt gatggttatt aaaccaatca ccagcaagaa gtgaaaaaac tgtgagtaag
    24121 cgggtgataa atggtaaaag tcatcttgct ataacaaggc ttgcagtgga ataatgaggc
    24181 cgtttccgtg tccatccttg ttaaaacaag aaattttacg gaataaccca ttttgcccga
    24241 ccgatcatcc acgcagcaat ggcgtaagac gtattgatct ttcaggcagt tagcgggctg
    24301 cgggttgcag tccttaccgg tagatggaaa tatttctgga gagtaatacc cagtctgttt
    24361 ctttgataat tgcgctgttt ttccgcatga aaaacgggca accgacactc tgcgcctctt
    24421 tgagctgacg ataaccgtga ggttggcgac gcgactagac acgaggccat cggttcacac
    24481 ccggaaaggc gttactttgc ccgcagctta gtcgtcaatg taagaataat gagtaagtta
    24541 cgatgaaaag aatgttaatc aacgcaactc agcaggaaga gttgcgcgtt gcccttgtag
    24601 atgggcagcg tctttatgac ctggatatcg aaagtccagg gcacgagcag aaaaaggcaa
    24661 acatctacaa aggtaaaatc acccgcattg aaccgagtct ggaagctgct tttgttgatt
    24721 acggcgctga acgtcacggt ttcctcccac taaaagaaat tgcccgcgaa tatttccctg
    24781 ctaactacag tgctcatggt cgtcccaaca ttaaagatgt gttgcgtgaa ggtcaggaag
    24841 tcattgttca gatcgataaa gaagagcgcg gcaacaaagg cgcggcatta accaccttta
    24901 tcagtctggc gggtagctat ctggttctga tgccaaataa cccgcgcgca ggtggcattt
    24961 ctcgccgtat cgaaggcgat gaccgtaccg aattaaaaga agcactggca agccttgaac
    25021 tgccggaagg catggggctt atcgtgcgca ccgctggcgt aggcaaatct gctgaggcgc
    25081 tgcaatggga tttaagcttc cgcctgaaac actgggaagc catcaaaaaa gccgctgaaa
    25141 gccgcccggc tccgttcctg attcatcagg agagcaacgt aatcgttcgc gcattccgcg
    25201 attacttacg tcaggacatc ggcgaaatcc ttatcgataa cccgaaagtg ctcgaactgg
    25261 cacgtcagca tatcgctgca ttaggtcgcc cggatttcag cagcaaaatc aaactgtata
    25321 ccggcgagat cccgctgttc agccactacc agattgagtc gcagatcgag tccgccttcc
    25381 agcgtgaagt ccgtctgccg tccggtggtt cgattgttat cgacagcacc gaagcgttaa
    25441 cggccatcga catcaactcc gcacgcgcga cccgcggcgg cgatatcgaa gaaaccgcgt
    25501 ttaacaccaa cctcgaagct gccgatgaga ttgctcgtca gctgcgcctg cgtgacctcg
    25561 gcggcctgat tgttatcgac ttcatcgaca tgacgcctgt acgccaccag cgtgcggtag
    25621 aaaaccgtct gcgtgaagcg gtgcgtcagg accgtgcgcg tattcaaatc agccatattt
    25681 ctcgctttgg cctgctggaa atgtcccgcc agcgcctgag cccatcactg ggtgaatcca
    25741 gccatcacgt ctgcccgcgc tgctccggta ccggtaccgt gcgtgacaac gaatcgctgt
    25801 cgctctctat tctgcgtttg atcgaagaag aagcgctgaa agagaacacc caggaagttc
    25861 acgccattgt tcctgtgcca atcgcttctt atctgctgaa tgaaaaacgt tctgcggtga
    25921 atgcgattga aacgcgtcag gacggtgttc gctgcgtgat tgtgccaaac gatcagatgg
    25981 aaaccccgca ctaccacgtg ttgcgcgtgc gcaaagggga agaaacgccg accttaagct
    26041 acatgctgcc gaagctgcat gaagaagcga tggcgctgcc gtctgaagaa gagttcgctg
    26101 aacgtaagcg tcctgaacaa cctgcactgg caacctttgc catgccggat gtgccgccag
    26161 cgccaacccc agctgaacct gccgcgactg tcgtagcccc agcaccgaaa gctgcaacgg
    26221 caacaccagc agctcctgca caacctgggc tgttaagccg cttcttcggc gcactgaaag
    26281 cgctgttcag cggtggtgaa gaagccaaac cgaccgagca accaacaccg aaagcagaag
    26341 cgaaaccgga acgtcaacag gatcgtcgca aacctcgtca gagcaaccgc cgtgaccgta
    26401 atgagcgccg cgacacccgt agtgaacgta ctgaaggcag cgataatcgc gaagaaaacc
    26461 gtcgtaatcg tcgccaggca cagcagcaga ctgccgagac gcgtgagagc cgtcagcagg
    26521 ctgaggtaac ggaaaaagca cgtaccaccg acgagcagca agcgccgcgt cgtgaacgta
    26581 gccgccgccg taacgatgat aaacgtcagg cgcaacaaga agcgaaggcg ctgaatgttg
    26641 aagagcaatc tgttcaggaa accgaacagg aagaacgtgt acgtccggtt cagccgcgtc
    26701 gtaaacagcg tcagctcaat cagaaagtgc gttacgagca aagcgtagcc gaagaagcgg
    26761 tagtcgcacc ggtggttgaa gaaactgccg ctgccgaacc aattgttcag gaagctccag
    26821 ctccacgcac agaactggtg aaagtcccgc tgccagtcgt agcgcaaact gcaccagaac
    26881 agcaagaaga gaacaatgct gataaccgtg acaacggtgg catgccgcgt cgttctcgcc
    26941 gctcgcctcg tcacctgcgc gtgagtggtc agcgtcgtcg tcgctatcgt gacgagcgtt
    27001 atccaaccca gtcgccaatg ccgttgaccg tagcgtgcgc gtctccggaa ctggcctctg
    27061 gcaaagtctg gatccgctat ccaattgtac gtccgcaaga tgtacaagtt gaagagcagc
    27121 gcgaacagga agaagtacaa gtgcagccga tggtgactgg ggtccctgtc gccgccgctg
    27181 tcgaacctgt tgttagcgca ccggttgttg aagaagtggc tgaagttgta gaagcccccg
    27241 ttcagattgc cgaaccgcaa ccggaagtgg ttgaaacgac gcatcctgaa gtgattgccg
    27301 ccgcagtaac tgaacagccg caggtgatta ccgagtctga tgttgccgta gcccaggaag
    27361 ttgcagaaca cgcagaaccg gtggttgaac cgcaggaaga gacggcagat attgaagaag
    27421 ttgccgaaac tgctgaggtt gtggttgctg aacctgaagt tgttgctcaa cctgccgcgc
    27481 cagtcgtcgc tgaagtcgca gcagaagttg aaacggtagc tgcggttgaa cctgaggtca
    27541 ccgttgagca taaccacgct acggtgccga tgacgcgcgc tccggcaccg gaatatgttc
    27601 cggatgcacc gcgtcacagt gactggcagc gccctacttt tgccttcgaa ggtaaaggtg
    27661 ccgcaggtgg tcatacggca acacatcatg cctctgccgc tcctgcgcgt ccgcaacctg
    27721 ttgagtaata attagctcaa gtaatcaagc cctggtaact gccagggctt ttttatttca
    27781 tctttgaatc aacatattaa aattcatcga atggcatcct tgctaaccaa caatgcaaaa
    27841 taggcaaaaa aatgcccgcc ccggcgatcc cagagcaggc agacaaaaca tacccagttt
    27901 catgatatgt tttaaagcga aattatttgt tgagctggaa gagcgacaat ccctgcatat
    27961 cggtaaatgc tttatacgat gcctgcaatg ccgtttgctg catgatgtaa gatgaaatag
    28021 ttgcattcca gtccacatca accagatcgc tcatctgctg cgtttgccct aaagcgcgat
    28081 cgctacctaa tgaatccagc gactccagtt cgttcagctg cgtgcctaat tccgcgcgaa
    28141 cggtcagcac attgttcagc gagtttttca gtccgcggtt ggttttatct aacgccgcag
    28201 cggcggtttc tttatccgct tcgctatccg cgaccggcgt tttcagggct gcgatggcgc
    28261 tatccagcat ggcaaaaaga ttggtttcag aagcgctacc gtctggttcc gctaccgcgt
    28321 tactggtaat actgtcgaaa attttgtcgc ccgtatgtcc tatcaccatc gaacgcgaag
    28381 catcgacctg ttgcttaata ctttctgttc cgccgacata atcaccgtta gcctcactaa
    28441 acggcgcagt ctctgtttta taaccggcaa aaatgtagcg cccattaccg tcagtggtgt
    28501 tcgccagatt cagcaactgg tcacgaagcc cctgaatatc cgtagccagc gaggcccggt
    28561 catcgtcact caaggtgcca ttgctggcgt agacaatttt ttcctgagca ttctggatag
    28621 cagtggtgac ctggctaagt acactctctt ccagtgacac tttttgagtg gcgaaagtac
    28681 gcgccagcgt gtactggctg ttttgcgcct gtgcctggga gagaacaacc gcttgtgatg
    28741 cagcaatggg atcatctgaa gggttaacga ctcgcttacc cgtcgacatc tgttcgccgt
    28801 acttcatcca ttctgcctga gaattggtga taccacgcat gttttgctgg tacatcatct
    28861 gggtactgaa acgcatttta tctcccctta gcgaatgtta atcagcgcat caaaaatcgc
    28921 gtttgccgtc tgcagaacct gcgcattcgc caggtaatac tgctgaaaac gttgcagatt
    28981 tccgtactcc tcatcgagat tgacaccgga aatcgactgc tgctgattgg aaagctgcgt
    29041 caccacatta ccttgcgtgg tgctactggt tttcaacgtc gcggttttat taccgatatc
    29101 actcactaac gaggcataag cgtcgttaaa ggatttcgca ccgcccaccg ttttactatt
    29161 gctttgcaga tccagcaggg cctgaccgtt gcggttatcg ctatcacccg catctttttc
    29221 gctcgccatc gctattttcg cttcgtcggt gattaataca tccatgttga cgatggcgtc
    29281 acttactggt ttcagcgtga agctgtcgtt aacggcaggc gttcctgtaa acgtcaactc
    29341 cagaccatca aatgccactt taccgttggc atccggcgtc accgtaaaag tggtattgct
    29401 ggcaaggcgg gtgacctgcc actgattatt atcgaacgag attttgtaat ctgtcgccag
    29461 taccacggag gcatcagtta ccgtggcacc gatcgcaacg tcacctttgt ttttcgtgtt
    29521 ttgcagaacc gcgggcttac cgatagtaaa gaaatcttca ccagcatcgc cgttagcatc
    29581 aaatccggct ttgtgttggg tgttgaaagc ctcggcaaat gccagcgcca gttgtccaag
    29641 cgtattacgc gtctggtcca gctcctgaga acggaatgtc agaatgccgc ccagcgaccc
    29701 ggtattcagt aatttctccg ggatctcaat attgcctgcc gtcccatcaa cataagcgac
    29761 agtcgtacga gaagggtcag cgctggaagg aactgccgcc agttgccgcg ccgtacttcc
    29821 ctgaaccagt gagtaaccat tggccaccgt gatgttataa gtgccgccat cctgaacgct
    29881 gacttctaca ccaacaatct ggtttaattc gctcaccagt tgatcgcgtt gatccagcag
    29941 attgttaggt gacgcccctg cccccacgcc tgtcaggcgc gagatttgat cgttcaggct
    30001 ggcaatttgt ttagcgtaat tgttgatctg atcaacgctg gcacctatcg cgatattgac
    30061 ctgtttgtcc tggtcgcgca gatattgatc ggtggtttta aactgattca ccaacccttc
    30121 tgatttccca atcagcgcct ggcgcgctgc cgggtcttcc gcgttactca ccagcgtttg
    30181 caggctggtg aagaaatcct gcatctgtgt tgccagcgaa gaggtactgg tggagagcat
    30241 attgtcgatt ttcgacatct gctcatagcg ggcggtcagg ccgctacttt gcgtctgcgc
    30301 agcacgtaac tggttggtaa taaacgcatc atactcacgc tgcacaccag aaacgtagac
    30361 gccattgcca acccagccgc cagcgcccaa cgtgctattg gcctgcgcca taatagtggt
    30421 ttggcgggta tatccggcaa cgttatagct ggagatatta ttacttgccg tatttaacgc
    30481 cgcctgggcc gcgttcagtc cgctcatggc gttattgatc aagctggaca tggaggttcc
    30541 ttataagcct tcaattacag agtattatcg gcagcgaccc gccggacttg agttattcag
    30601 aacagattat caatgttcat gctgtaggtt ttgctcacct tgtcgcttat cgatttcatc
    30661 tgctggatca tgttggtgag tttacgggca tagtgaggat cggtagcata ccccgcgtcc
    30721 tgtagggcct gcgccccctg ctccgcactc gcggctgtcg tcacggcggc gtagcgcggg
    30781 ttacgtgtta acagcccaac gtaatccgac aatgcttcca gatacgagct gtagacccga
    30841 aatttcgctt ttactttctt cgcttcgcca ttttcatatt cagtcgtggt gatttcagta
    30901 actggacctt tccagttgcc agaggctttg acgccaaaca ggttatagct cggctcgccg
    30961 ttttcgcggc ggatttgccg ttgcccccaa ccagactcca gcgccgcctg agcgaggatc
    31021 aaatgatgtg gcacaccgct ttgctggctt gccagttggg cgggcagcga gagttgcgcg
    31081 aggaatgctt tactgtcacc cggcagcgaa tcatcgtagt tacgtggcac ggccttttgc
    31141 accagctgcg aaagcgcctg attttgataa cgcaccacag tttcgagcgg gaatttcatc
    31201 ggtgctgctg gcgtggactc ctctggcaat ggttgttctg gcgtcatctg tttaaccatc
    31261 atctctgcaa gccccagacc tttgcccgcc gtcatctgtt gggcaatctg ctggtcatac
    31321 atactggtat acaggcgagt gtgctcgctg ctgaacaggc catcttttgg taaagcgtcg
    31381 cgcatgcttt tcaacatcat ctgcacgaac atcccttcca cctgacgggc caccggacgg
    31441 atatttgccg ccggatcttc gcccgctttc gcctttagtt cgttgagtga ttgcgcatcc
    31501 caggccgcac ttgccagtag tttgctgtcg ctgatcatca gatgatttcc agttttgccc
    31561 gcagacatcc cgcactttgc attgattgca gtatggacat cagatccatc ggcgtagcgc
    31621 ccagcgcatt gagcgcgcgc accacgttat tgaggctggc gctggaacgt acgctttgca
    31681 gcgaaccgcc gctctggcgt aaatcgatct gcgtttgtgg agtaaccaca gtctgtccac
    31741 caccaaacgg tgtatctggc tggctgacat tggcctgacg attgactgtt actgagagat
    31801 ttccctgcgc taccgcgcag ctgtcgaggg tcacttcgcg attcatcacc accgaaccgg
    31861 tgcgcgagtt aatcactact ttagcgtcct gcggggtgac attaacctgc atattctgga
    31921 tatctgcaag gaaacggacc tgggaactgt tgccacttgg tacgcgcacc tgaatagtcc
    31981 gcgcatctaa cgcggtggcg ctgccatatc cacgcacgcg gttgatggtg tcagcgattt
    32041 gctgcgccat gctgaaatct tcgtcgttaa gttgcaaatt aagggtattc ccgacgccaa
    32101 actggctggg caattcacgt tcaataaccg caccattggt gatccgtcca ccgttcagtt
    32161 ggttcacctg aacactgcta ccgccagcgg aggctcctgc gccgccaacc agaatattgc
    32221 cctgcgccag cgcatacacc tgactgtcaa cgcccttaag cggcgtcatc aacaacgtac
    32281 ctccacgcaa gcttttggca tttcccatgg aagaaaccac cacatcgatg gtttgcccct
    32341 gacgtccaaa cggaggaagt gactctgtca ccattaccgc agcgacgttt tttagctgca
    32401 tattggtgcc cgtcggaacg gtaattccca gctgtgagag catgttatta agcgtttgtg
    32461 tggtaaacgg cgtctgggtt gtctggtcac cggtgccatc cagccccacc accagaccat
    32521 agccaatcag tgagttttgc cttaccccct gaacactggt gagatcgcga atacgctcag
    32581 cctgagccgc cgtcgtaacc agtagaagaa ttaatgcaga gagaaattta atcaccacag
    32641 cctcacttac attggcgaca ggttaaggaa gaaacgctgc aaccagccca tattttgcgc
    32701 ttcgttaatg tagccattgc ctacgtattc aatgcgcgca tccgccacct gagtagacgg
    32761 tacggtattg ctgccgctga tagtgcgtgg attaaccacg ccagagaagc gaataaattc
    32821 ggtaccctga ttaatggcaa tctgtttttc acccaccaca tgcaggttgc cgttgaccag
    32881 tacctggtca accgtcaccg tcaacgtgcc gctaaaggta ttgctggcat tggccccgcc
    32941 ctttccgttg aacgtgttac caccggaggc ttcgacatcg gcacgagcgt taccaaacag
    33001 cccctgcaaa tagcgcggca cagtatcaaa gccaaaatta gttttaccgt cacggctggc
    33061 attcgcagag gagcttttgc tggcgctgac gttctcctgc aacacgatgg tcagcgtatc
    33121 gccaatattg cgtggtcgac gatcttcaaa cagcggttga tagccatagt taatcggctg
    33181 agcagactgg aaaatagaac cgttggcgac gggcgtcgga ccgggaaccg gttgtgcact
    33241 ggtcgccccc tgcaccagcg gcgtggaggg tatccaggcg cagccggtta gtgaaagcac
    33301 caacaagctg gaaatggcat aagtatgcgc agcgtttttt tgcatggcta tcatcttcaa
    33361 aaatcagtaa accggtgaac ctgccaccgg ttaagcctta gagttgcgtc agtttttgca
    33421 gcatctgatc ggtggtggac accgctttac tgttgatttc gtaagcgcgt tgcacctgaa
    33481 tcatattgac cagttcttcc gccacgttga cgttagacgt ttcaacatac ccttgataca
    33541 gcagtcccgc gccgttcagg cccggcgtgc tttcgttcgg tacaccagag gattgcgttt
    33601 cggtgtagag gttttcgcca atgctctcca gcccggtgtc attcataaag gtggtgagat
    33661 tgagctgccc aacctgaacc ggagctgcct ggccttgttg ggttacgctg accacgccat
    33721 cacgaccgat ggtgatactt aacgcattcg ccggaatggt gatcgctggc tgcacctgaa
    33781 aaccaccagc tgcccgttct gatccacctg gaaagagccg tcacgggtat aggctgatga
    33841 accatctggc aacatcacct ggaaaaagcc ctgcccttta atcgcgacat ctttgctgtt
    33901 gttggtctgc gacaggtttc cctggctgtg taagcgttca gtggcgaccg ggcgtacccc
    33961 cgtgccgatt tgtaatccgg agggtaaggt ggtttgttcg gaagactgtg cccccggctg
    34021 gcgaatggtt tgataaagca gatcttcaaa caccgcgcgc tgacgcttaa aaccgttagt
    34081 actgacgttt gccaggttgt tggcaatgac gtccatattg gtttgctggg cgtcaaggcc
    34141 cgttttggcg atccataatg aactgatcat aaaatgtcct gtagtggatt agtgacaaag
    34201 tgcatatgtc ttcttgccgg atgcggcgta aacgccttat ccggcctaca agtgctcgca
    34261 aaatcgataa attgcgattg cccccataga cccgataaac ttgcgcatcg ggcaacttat
    34321 acttgtcatg tatcctttca attaactcat cgacagcagt tggttggcac ggcctgcgtt
    34381 atcatcgacg ctgctgatca ccttcatctg catttcaaaa cgccgcgcgc tggcaatcat
    34441 gtcgctcatt gccgcaacgg cattgacgtt actgccttcc agaacccccg acatcacacg
    34501 caaggttgga tctgcctgca gtaccggccc tcgcgtggcc tgggtttctg cgcttaaacg
    34561 aaaaatgccg tcgtcaccgc gctgcacttc gctgcccgtg gctttcacca gtttcagacg
    34621 ccctactggc gcaactgtat ttgccggatc gcccggattg agcgccgaga ttgtgccatc
    34681 ggcagcaata gtgatttccg ctccttccgg cacagcaatt ggccccgcct cgcctatcac
    34741 ctgatgcccc tgaattgtca gttgcccggt gggatcaacc tgaatgctgc cattacgcgt
    34801 atacccttcg ctgccgtcag cggtctgcac ggccagccag ccatcctgct gcaacgcgac
    34861 gtccagcggg cgcgaggtgt aatccatttt gccgggcgtc atatctgcgc ccggcgttga
    34921 cgccgtgacc aacgtgcgcg tgggcagaga aagcccttcc actggcaccg cgcgtaaagc
    34981 attcaactgc gcgcgaaaac cgggcgttga ggcattggcc agattactgg cggttaccgc
    35041 ctgttgattc agtgtctggc tggctgctcc catcgcggta taaattgcgt gatccattga
    35101 gctatcccgt cagcgattag cgtaagttaa ccagcgtgtt gaggatctgg tcctgagttt
    35161 tgatggtctg ggcgttagac tgatagttac gctgggcaac gatcatattg accagttctt
    35221 tactgagatc gacattggac gcttccaacg caccgttggt cagggtgcca aagttgcccg
    35281 tcccggctgt ccccaacagc gccacgccag aagattgcgt cgcagaccag acgttgtcgc
    35341 cttcggatgc cagaccttcg ttgttggcaa agttcgccag tacaatctgc cccagcagtt
    35401 gtgtttgttc gttggaatag ttgccgacaa ccgtaccgtc atcattgatt tgataactca
    35461 ccagatcgcc cggtttgtag ccattctggg tggttgccac aatattgtta gcgccggtat
    35521 tttgctgcat ggagttgagg aagctcaggc taaacgtggc gggatctgcg ccgttaattg
    35581 cgccagtggt aatattctct gttggatttg aggtcagaac gccattggca ttaaacacca
    35641 gcttcattgc aggctcggct gtacctgttg gatcactgct atcctgggtg tagacctgcc
    35701 agttattatc cccggtcttc acaaagtaga cgctcatgtc atgagcatta ccctgactgt
    35761 cgaaaacagt caccgaacct tttttgttat agctatccgc attgctggcg ctgaatggcg
    35821 taacagtagg aagcggatca ctggaattca ggttgatctg catcgacgcc gtggtggtag
    35881 ttttcgctgc catcagggta ttcgggatcg aaatattggt cggattcgcc ccttgctgaa
    35941 tagtcggcgg cgtaccggtt gccgggtaac ccgtcagctg taaaccttgc atattcacca
    36001 ggttacggtt ttcatccagc ttaaattgtc cgttacggct gtagaacacc gaaccgttgc
    36061 tgtctaccag acggaaaaaa ccgttctggc tgatagcaac gtccagacct cgcccggtgt
    36121 tggtggtcgt gccatcggta aagtcctgag tgataccggc aacttttacc cccagtccca
    36181 ctttcgaacc ggcaaacata tcggcaaaag aggccgtgcc tgatttaaag ccgtaggtgg
    36241 cggagttggc gatattgttg ccaataacat cgaggttggt ggcggcagcg tttaatccgc
    36301 taaccgcttg agaaaaggcc atgactgact cctgaaatgt gaaggcttaa attatctgcc
    36361 gtacttcgtc gagggtggtg gtgccgtaag tgccgagatc cagcgtatta ccgctgttgc
    36421 cgcggagcac accctgcacc agagcaaact gcagcggctg ggcaaccagt tgtgtaccac
    36481 cgttactggc gctaatcgct acattgtaag aaccgttcgg cgcagtgctg ccatcagtca
    36541 acgtaccgtc ccaggtgaaa ctgtggactc cggcggtcag ttcaccaata tcaatggtgc
    36601 gcacaaccgc gccattttta tcggtgatgg tggccgtaac tttgtctgcc gcctgttgca
    36661 gctcaacacc aaacggcgtg gtcgtggtca cagccccttc ttcactgccg gttcccgcaa
    36721 gaacagtggt gccggggatc atcacgccgt gaccgatcag gttactggcc tgtaacgact
    36781 ggctgttatc aatctgtccg gaaatagatc cgagcgtggt attgagtttt tcaatcccgc
    36841 tgaccgtgct gatttgtgcc aattgcgacg tcagctcgtt gttttccatt ggattggtcg
    36901 ggtcctggtt tttcagctgc gccaccaaca aagtcagaaa actgctttgt aagtctgcgg
    36961 cgttgctgcc cgtgagcgaa ctactgctgg tggtactgac gccggtattt gtcggatcgg
    37021 tggtggttac cgcaatggac atagctttct cctttattga ccgagcgtaa gggttttcag
    37081 catcatgctt ttcaccgtgt tgagcacttc aacattggcc tgatagctgc gtgacgccga
    37141 catggtgtta accatctccc cgacgacatc aacgttcggc atttttacgt agccctttgc
    37201 atccgccagc ggattacccg gttcataaac cagtttgtcc ggggcctgac tttctataac
    37261 atcggcaacc tttacgccgc ctgtcgcagc acctggtgca gcgttaacct ggaataccac
    37321 ctgttttgcc cgatatggct gtccatcggg accggtcacg ctatcagcat tcgccagatt
    37381 actggccgcc acgttcaagc gctgggactg ggcagttaac gccgacccgg cgatatcaaa
    37441 aatattcagc agtgccatcc gttaatttcc gctctgtaaa acgttcatca tgcctttgat
    37501 ttgcccgctc aacgcgctaa ggctcatctg gtattgcagg ctgttatcgg caaactgggt
    37561 gcgttcgcga tccatatcga cggtattacc gtcaagcgaa ggctggtccg gaatacggta
    37621 ttgcagttct gcggtaggag gcgtcagcgc ctgcgccgga atgtgttgcg ttgaggtcat
    37681 cgtcagtgca accacactgg ttgcatcccg tccacgttgc atgacttttt taagttcact
    37741 ggcaaaatcg atatcgcgcg cctgataacc aggggtatcg gcattggcga tgtttgctgc
    37801 cagcacttcc tgacgctggg cgcgcagatt gagcgcctct tgttgaaaac gtaaggcggc
    37861 gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa ttctgccatc agcttaaatg
    37921 cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg gtcgctattt atgccgttga
    37981 tgaccattgc ggacaggtac aattcacgtt gtagaaatgg ctgggggcga aaatgctggc
    38041 aataaaacgt agcgtggcga tcatctcgat actattcact ccgttaagtg cggcgagtaa
    38101 tctcacatcg caattgcaaa ccttttttag cgcccagctc gcgggggtaa gtgatgaggt
    38161 tcgtgtttct attcgtacag cgcccaatct actaccgccg tgcgagcagc cattgctttc
    38221 gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg ttggcacgct gcggtaacga
    38281 caaacgatat ttacaggtta atgtacaggc cacaggaaat tatgtggttg ccgcgatgcc
    38341 cattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa ctaaaacgcg gacggcagga
    38401 taccctgcca ccgcgtacgg tgctggatat caatcaactt gttgatgccg ttagcctgcg
    38461 cgatctatca cccgatcaac ctatccagtt aacccagttt cgccaggcat ggcgggtaaa
    38521 agcgggacaa cgcgtcaacg tgatcgccag cggtgacggg tttagcgcca acgcagaagg
    38581 tcaggcgctg aacaatgcag ccgtcacaca gaatgcgcgg gtgcgcatgg tatcgggact
    38641 ggtggtcagc ggcgttgttg atgcagatgg gaatattctt ataaaccagt aagctgttaa
    38701 agattacccg tcccttgccg ataaataagc aacacatgat aaaagcgccc tcaatgagga
    38761 ataaaccatg agtattgatc gcacttcgcc tctgaagccc gtaagcaccg ttcaaccacg
    38821 cgaaaccact gacgcgtcgg taacgaacac ccgggcggca aaaacaacag cctccaccag
    38881 caccagtgtg acgttaagcg acgcgcaagc aaaactgatg caacccggca gcagtgatat
    38941 caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt aacggtgaac taaaaatgga
    39001 caccggcaaa attgccgatg cgctgctcaa cgaagcgcag caagacttgc agagtaactg
    39061 atcgtatgac acgtcttgca gagatcctcg accagatgtc cgctgtgctt aacgatctca
    39121 aaacggtaat ggatcaagaa cagcaacatc tctctatggg gcagatcaac ggcagccagt
    39181 tgcaatggat tacagaacaa aaaagctcac tgctggcgac gctggattac ctcgaacagt
    39241 tacgcaggaa agaacccaat acagcaaata gcgttgatat tagtcaacgc tggcaggaaa
    39301 ttactgtgaa aacgcagcaa ctacgccaaa tgaatcaaca taacggctgg ttactggaag
    39361 gacagattga gcgcaatcaa caggcgctgg aaatgttgaa accgcatcag gaaccgacgc
    39421 tatatggggc gaacggtcag acctcaacaa cccatcgcgg cggtaaaaag atttcgatct
    39481 gaatattatc tccggcctgc actgcaggcc ggaatgcatt gttacaccgt ccggcgggca
    39541 aattctttaa ctttgaagcc cagtaccgcc agtgcagcga agtacgcggc aatccccgcc
    39601 agcacgaccg ccattaaacg cagtaagcgc cagggcatgg tacccaatga ccactccggc
    39661 atgatatgta acatacctaa aagcacgcca gacatcacca gtaccgccac caccagacgc
    39721 aacagaaacg ccatccagcc gggttgcgga gtaaagattt tctgcttacg caactgccag
    39781 taaagcagcg aagcattcag acacgccgcc agaccaatag aaagtgacag cccagcatgt
    39841 ttcaacggac caataaacgc caggttcatc aattgcgtta aaatcagcgt aacgatggca
    39901 attttcactg gcgttttaat gtcctggcgg gaataaaagc caggagccaa cactttcact
    39961 acaatcaggc caatcaaacc caccgagtag gcaattaacg cccgctgggt catcagcgca
    40021 tcaaacgcgg taaatttacc gtactggaac agcgaaacgg ttaacggacc cgaaagaatg
    40081 cccaacgcaa ccgcactcgg cagcgccaac aggaaacaaa gacgcaaccc ccagtccatc
    40141 aaacggttgt attcatcatg attgccactg gcaaaacttt tcgacagcga cggcagcaaa
    40201 atggtgccaa gcgccacgcc cagcacaccg gagggaaact ccattaagcg gtcggcgtaa
    40261 tacatccaag acaccgaacc ggaagcaaga aacgaggcaa aaatggtgtt gatgattaag
    40321 gagatctggc tcacagagac gccaaggatc gccggtccca tctgtttcac cacgcgcatt
    40381 gctccggcat cgtggaagtt aatgcgcggc aggaccagca tgccgatctt cttcaggtgc
    40441 ggtagctgat acaccagctg caggatgccg ccgaccgtaa cagcccacgc cagcgccagc
    40501 accggtgggt taaagtacgg tgcggcaaac agcgcgaaac caatcatgct gatgttaagc
    40561 agtgttggcg caaacgccgg aatcgagaag cggttccacg tattcagaat cgctcccacc
    40621 agcgacgcca gggagatcag caagatatag ggaaaggtaa tctttagtag ctggctggtc
    40681 agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg tcaccatgat cacccacggt
    40741 gcggcgagca tgccagcgac cgtcacaacc gccagcgcaa gtgtcagcag gccagaaaca
    40801 taagagacaa agacccgcgt ggcgtcttca ccctgcttac ttttatattc cgccagaatc
    40861 ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac ggcgtaacaa gttaggaagt
    40921 ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc caaagattct ggcgacaatt
    40981 gcgtctcgtg cgaagccaag cacacgcgaa aacatggtca tcgagctgac ggcggccagc
    41041 gattttaata aattcatcgg tgttctaatc cagacccacg ggcaaaacgc ctgcaaggca
    41101 ggcgctgaaa gaggcgaata gtctacctgg attatggtga attgctaccg ccagatgtta
    41161 cagggtttat tcactcatcg catcgcgcca gatcttgtca acgatacgtt gcgccagcac
    41221 tgcctgttcg ccggcggttt gcagaactgt ctgattttgc acacattcaa tgaagtgccg
    41281 cgcacagccg acaaatccac gttgctcaag agtgctctgc caaccaggaa tcggtttatg
    41341 cactacgccc tgcccgcgct cctcacgcca ttcgcgcata tccgtaatgt cgatgagcgc
    41401 atcgtcagtc acggcctgca cggtttcacg ctgacttccg gcacggcgat gcatacaggt
    41461 ggtgatctgc aaaggatcgg ccgaaaaatg gtgctcggca aacagcattt cgccagcgtc
    41521 gttagtcagt agcgtaccgc catccagaga ggctttgccg cccgacaacc acagcgcggt
    41581 atccaccaca tgcagataat catccagcaa cgtgaaataa agatcgtgtg gcccgacact
    41641 attgctacga tgtttatcca ttcttagcga agctgcggta gcgagttgcg tttttaactc
    41701 accgtagagt ggtgcgaaac gacggttaaa accgaccatc aacgtcagtt ttttccgcgc
    41761 cgccagttcc accagccgtt cagcatcgcg cagattttct gccagcggtt tatcgacaca
    41821 gacatgtaca cccgcattga gtaacgtact gaccacgtca aagtggctgg cggtgctgga
    41881 atgcacaaaa accgcatcgc aactggcggc aaggctggat aacgaatcgg cataaggaat
    41941 gcgccagctt tcacaaattg gcagggcttt cgcgcgcgta ggcgaccagg ctccttgtaa
    42001 catccagtca gacgctgccg ccagcaccgg taaccacgct ttttgcgcaa tgccgcctaa
    42061 tcccacaacg ccgatacgta attttttcac tttaatctcc cagatgggcc agcaacgaat
    42121 caagacgctg tttcagttct gccacttcga tttccagggc ttcgacgcgg gcctgtaaat
    42181 caccatcaac cgcgttcgac atatccgtta ccgccggctg atcctctacc tcaccactga
    42241 aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg ggccagacgc accacaaaag
    42301 gaccatcttc gcgatttgcc agttgttcca gcgtcgactc cacttccgcc atatcgctga
    42361 attcatacat tcgcgcggcg cggctgcgca gttcacccgg cgtctgggca ccacgcaata
    42421 acaacgtggt gattaacgcc acttccgctg cgctcagttt cagatcgcca aattctgaat
    42481 tacaaaaacg ttgttcatat ttggtgaccc gattaccaaa gccactcacc gtgcgcagat
    42541 aatggcgttt gaccagatta tccagttgct cctgcacttc agattcactc agattcatga
    42601 ccggttcacg gttcgttttc tgattacagg ccgtgactac accattgact gagagcgggt
    42661 attgctccgg cgtggtcacc tgcttttcca gcaagcaacc aatcacgcga gcttcaaggg
    42721 cagtaagttg atatttcatg agattctcct tagcggccgg gcgtccagtc tggggtagtt
    42781 aatgccgtca gtacgtgatc gcgccattgt ccatcaatca acagatagtc tttcgcatag
    42841 ccttcttttt caaaacccag tcgcgccagt aaatcaccgc tgcgtttatt gtgcggcata
    42901 taattagcca taatgcgatg aatatgttgg gtgcgctgca tataacgaat ggctgcggtc
    42961 agggcttcaa acatgagtcc tttgccctgc catttttgcc caatcgaata accgagatag
    43021 caggcatgaa aagagccacg aacaacattg gaaaaattgg caacgccaat aatctctttt
    43081 tcgtccgggt cgaataagcc aaagtagaaa gctgaacctt gtttatgaaa ttcgttaatc
    43141 atccccagcc tggcctgcca gcctgatgga taacagtggc tttcgtcgcg cactggctcc
    43201 cagggcttga ggaaatggcg attctctgcg taataatccg caagacgcca ggcatcacga
    43261 tcatgcacca gacgcacgac cagtcggtct gtggttaagc gcacttttgg cacgttactg
    43321 cgatagccaa acattctata cctactcctt cccgtaacgt ctttaatacg ctgtaagtat
    43381 tactatacct gcgcaaccag accctgtgaa aagtccaaaa tgccattttc tgcattattc
    43441 acatatttga taaaaactct ctatcaaagc gacgttttga taaaaaaata ttgtccccga
    43501 agtgatggga aaagtcttag cggcaatgca gaatagaaag tgctcatctt ttatttcccg
    43561 ggaggggaaa tgtcccgcgt atcgcaggcg aggaacctgg gtaaatattt cctgctcatc
    43621 gataatatgc tggtcgtgct ggggttcttt gttgtcttcc cgctgatctc tatccgcttc
    43681 gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg ctctcggtct acgccagttt
    43741 attcagcaag gtctgggtat tttcggcggc gcaattgccg accgctttgg tgccaaaccg
    43801 atgattgtta ccggtatgct gatgcgcgcc gccggattcg ccacaatggg tatcgcccac
    43861 gaaccgtggc tattgtggtt ttcatgcctg ctctcgggac tcggcggcac gttgtttgat
    43921 ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac aacaacgttg tcgttttttc
    43981 tcgctgttga tgatgcagga cagtgccggt gcggtcattg gcgcattgtt ggggagctgg
    44041 ctgttgcaat acgactttcg cctggtctgc gccacagggg ccgttttgtt tgtgctgtgt
    44101 gcggcgttca atgcgtggtt gttaccggca tggaaactct ccaccgtacg cacgcccgtt
    44161 cgcgaaggca tgacccgcgt gatgcgtgac aagcgttttg tcacctatgt cctgacgctg
    44221 gcgggttact acatgctggc tgtacaagtg atgctgatgc tgccaattat ggtcaacgac
    44281 gtggctggcg cgccctctgc cgttaaatgg atgtatgcca ttgaagcgtg tctgtcgtta
    44341 acgttgctct accctatcgc ccgctggagt gaaaagcatt ttcgtctgga acaccggttg
    44401 atggctgggc tgttgataat gtcattaagc atgatgccgg tgggcatggt cagcggtctg
    44461 caacaacttt tcaccctgat ttgtctgttt tatatcgggt cgatcattgc cgagcctgcg
    44521 cgtgaaacct taagtgcttt gctggcggac gcaagagctc gcggcagcta tatggggttt
    44581 agccgtctgg gtctggcgat tggcggcgct attggttata tcggtggtgg ctggctgttt
    44641 gacctgggca aatcggcgca ccagccagag cttccgtgga tgatgctggg aattattggc
    44701 atcttcactt tccttgcgct gggttggcag tttagtcaga aacgcgcaac gcgtcgtttg
    44761 ctggaacgcg acgcctgatt tgatactccc ccctgactcc tccatactga aagtgaactg
    44821 acagccaaat ggaggagtca cgtgaagcta tacatttacg atcactgccc ttactgcctc
    44881 aaagcccgca tgattttcgg cctgaaaaat atccccgtcg aattacatgt tctgctcaac
    44941 gacgacgcag aaacacccac ccggatggtc ggtcaaaaac aggttcccat tctgcaaaaa
    45001 gatgacagcc gctatatgcc agaaagcatg gatatcgttc actatgtcga taaactcgac
    45061 ggcaaaccgt tactgaccgg caaacgttcc cctgccattg aagagtggct gcgcaaggtc
    45121 aatggctacg ccaacaaact gctgttgccg cgttttgcca aatcggcatt tgatgagttt
    45181 tctactcccg ccgcgcgcaa atatttcgtc gacaagaaag aggccagcgc gggtaatttt
    45241 gccgacctgc tggcccactc tgacggtctg attaagaata tcagcgatga tttgcgtgcg
    45301 ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg aactttcgga agatgatatt
    45361 cagctatttc cgctactgcg taatctgacg ctggtagccg gaatcaactg gccaagccgc
    45421 gttgctgatt accgcgataa tatggcgaaa cagacacaaa tcaatttgtt atcatcaatg
    45481 gcgatttaat cctgacccgc cgcggcagcc tgtattcgcg ggttgccgcc gttttcagtt
    45541 ttttctgctg gcgagtttat cgctggtgac acatgctgcg ttgtgtcagg atattgacgc
    45601 agatttcatg tcgaggacta ccatgaacaa gtttttattt gccgctgcat tgatcgtcag
    45661 tggcctgctc gttggctgta atcaactcac ccaatacacc atcaccgaac aagaaattaa
    45721 ccagtcgctt gcgaaacata ataatttctc aaaagatatc ggtttacccg gcgtggctga
    45781 cgcccatatt gttctgacaa acttgatcag ccaaattggt cgcgaagagc cgaataaggt
    45841 taccctaacc ggagacgcca atctggacat gaactccctg ttcggtagtc agaaagcgac
    45901 catgaaactg aagctgaaag cgctgcccgt gtttgataaa gaaaaaggtg cgatcttcct
    45961 gaaagagatg gaagtggtcg atgcgacggt acaaccggaa aaaatgcaaa cggtgatgca
    46021 aacgttgctt ccctatttga accaggcatt acgcaattac tttaaccagc aacctgctta
    46081 cgtcctgcgc gaagatggca gtcagggcga agcaatggcg aaaaaactgg cgaaaggcat
    46141 tgaagtgaag ccaggcgaaa ttgtcattcc gtttacggat taatgacgag ggcgcattcg
    46201 cgccctttat ttttcgtgca aaggaaaacg tttccgctta tcctttgtgt ccggcaaaaa
    46261 cattccttca gccggagcat agagattaat gactgcacca tcccaggtat taaagatccg
    46321 ccgcccagac gactggcacc ttcacctccg cgatggcgac atgttaaaaa ctgtcgtgcc
    46381 gtataccagc gaaatttatg gacgggctat cgtaatgccc aatctggctc cgcccgtgac
    46441 cacagttgag gctgccgtgg cgtatcgcca acgtattctt gacgccgtac ctgccgggca
    46501 cgatttcacc ccattgatga cctgttattt aacagattcg ctggatccta atgagctgga
    46561 gcgcggattt aatgaaggcg tgttcaccgc tgcaaaactt tacccggcaa acgcaaccac
    46621 taactccagc cacggcgtga cgtcaattga cgcaatcatg ccggtacttg agcgcatgga
    46681 aaaaatcggt atgccgctac tggtgcatgg tgaagtgaca catgcagata tcgacatttt
    46741 tgatcgtgaa gcgcgcttta tagaaagcgt gatggaacct ctgcgccagc gcctgactgc
    46801 gctgaaagtc gtttttgagc acatcaccac caaagatgct gccgactatg tccgtgacgg
    46861 aaatgaacgg ctggctgcca ccatcactcc gcagcatctg atgtttaacc gcaaccatat
    46921 gctggttggt ggcgtgcgtc cgcacctgta ttgtttaccc atcctcaaac gcaatattca
    46981 ccaacaggca ttgcgtgaac tggtcgccag cggttttaat cgagtattcc tcggtacgga
    47041 ttctgcgcca catgcacgtc atcgcaaaga gagcagttgc ggctgcgcgg gctgcttcaa
    47101 cgccccaacc gcgctgggca gttacgctac cgtctttgaa gagatgaatg ctctgcagca
    47161 ctttgaagca ttctgttctg taaacggccc gcagttctat gggttgccgg tcaacgacac
    47221 attcatcgaa ctggtacgtg aagagcaaca ggttgctgaa agcatcgcac tgactgatga
    47281 caccctggtg ccattcctcg ccggggaaac ggtacgctgg tccgttaaac aataaaaatc
    47341 gtagcttcct gttgtcatta ggttatttaa cctgtataaa taaccagtat attcaacagg
    47401 gggctattat gcgaattgaa gtcaccatag cgaaaacttc tccattgcca gctggggcta
    47461 ttgacgccct ggctggcgaa ctttcccgcc gtattcagta tgcgtttcct gataatgaag
    47521 gccacgtatc ggtacgttat gccgcagcga ataatttatc ggttattggc gcaacaaaag
    47581 aagataaaca gcgcattagc gaaattctcc aggaaacgtg ggaaagcgcc gatgactggt
    47641 ttgtcagcga ataatatgca gtgatttttg tttttgccgg gtcgccccgg cttttttatt
    47701 ttacttacga attttgagta caaaatttct acacttcctc aaaataattg cacaatatgc
    47761 taaaaaaata tgaatgacat gctgttttat tgttcatata aatcttaaaa gcagaaattt
    47821 atctgttccc ccctttaaaa attcggtgaa tacccttact tattggtata ctgaagttgc
    47881 ttcagataaa aacgcattga acctcgaata acgttgtcta gtaacacgaa ttagggggcc
    47941 atgatggaaa aaaataatga agtcattcag actcatccgc tcgtagggtg ggacatcagc
    48001 accgttgata gctatgatgc gctgatgttg cgtttgcact accagacccc aaataagtcc
    48061 gagcaggaag ggactgaagt tggtcaaacg ctctggttaa ccactgatgt tgccagacaa
    48121 tttatttcga tattagaagc aggaatcgcc aaaattgaat ccggtgattt ccaggtaaac
    48181 gagtatcggc gtcattaaca tgacatgacc atccacccaa aaggcacctc accggtgcct
    48241 ttaccatttc tgggttgtat atctgcattc agttaattac tctgcttaaa ccatgagagc
    48301 agagagaaaa aaatgaaata cgatctcatc attattggca gcggttccgt aggcgctgcc
    48361 gccgggtatt atgcaacccg cgccggttta aacgtgctaa tgaccgacgc ccatatgcca
    48421 ccgcatcaac acggcagcca ccacggcgat acgcgattaa ttcgccatgc ttatggtgaa
    48481 ggcgaaaagt atgtcccgct ggtcctccgc gcgcaaacgc tgtgggatga actttcccgc
    48541 cacaacgaag atgatcccat ttttgtacgc tctggtgtca ttaacctcgg cccggctgac
    48601 tccgcatttc tcgccaacgt cgcccacagc gccgaacagt ggcaactcaa cgttgaaaag
    48661 ctcgatgcgc aagggattat ggcccgctgg ccagaaatac gcgtcccgga caactacatc
    48721 ggcttatttg agactgattc cggttttttg cgcagcgaac tggcgattaa aacctggatc
    48781 caactggcga aggaagcggg ctgtgcgcaa ctgttcaact gcccggtcac cgcaattcgt
    48841 catgacgatg atggcgtaac tattgaaacg gttgacggtg agtatcaggc gaaaaaagcg
    48901 attgtctgcg cgggaacatg ggtaaaagac ctgctcccgg agctgcctgt ccagcccgta
    48961 cgtaaagtat ttgcctggta tcaggccgat ggccgctata gcgtgaagaa taaattcccg
    49021 gcgtttaccg gtgaactgcc caatggcgat caatattatg gttttccggc agaaaacgac
    49081 gcgttgaaga ttggcaaaca taacggaggc caggttatcc attcagcgga tgaacgtgtt
    49141 ccgtttgcgg aagtggtcag cgatggttcg gaagccttcc cgttcttgcg caatgtattg
    49201 ccgggtatcg gttgctgcct gtacggcgct gcctgcacct atgataattc gcctgacgaa
    49261 gattttatta tcgataccct acccggccac gataatacac tgctcattac cggcctgagt
    49321 gggcacggtt ttaaatttgc gtcagtttta ggggaaatag ctgccgattt tgcgcaagac
    49381 aaaaaaagcg attttgattt gacgccattc aggctttccc gcttccaata atcatcatta
    49441 cggcctccag aatgaggccg tttttacagg agtatttaat gcgtcggctg ttgcactatc
    49501 tca
//
LOCUS       DAZPAC010000005        47864 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZPAC010000005 DAZPAC010000000
VERSION     DAZPAC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.005"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..236
                     /locus_tag="ACHUPM_000217"
     CDS             <1..236
                     /locus_tag="ACHUPM_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868119.1"
                     /translation="SDIAYLNSFSEDDYLKWILSRNQDALDLVRKGLLRFKSMQELTE
                     EQQKITNKAIGALTKLASRSSLNKLRVSRLLNN"
     gene            417..1157
                     /locus_tag="ACHUPM_000218"
     CDS             417..1157
                     /locus_tag="ACHUPM_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868120.1"
                     /translation="MSQIFFDTIDNDQYDFMTEWNTAVMDKWVAENIGLSRCKDEAEL
                     FDTKWFDYRDMHPLMATCLFTEAYKRQYSNIMLTHGREHFETAPFTTGLKRLPYQELS
                     TANKTSLWKARQFADRYCCSYDYFISTVLSAAARRLWDKLPRPQHLWQPELIEIFEEK
                     LARRATTRLDDSLVSFKHMGDMQFNPIQESYFEWILERLRAIPRSKRIRAIFSAIWLM
                     EIVPERLISAHFPEELEEARWFIDPLYN"
     gene            1201..2541
                     /locus_tag="ACHUPM_000219"
     CDS             1201..2541
                     /locus_tag="ACHUPM_000219"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFR0868121.1"
                     /translation="MTELCHTGRGLSEEFDEDFQNRLTAYFCRDHEFLTRAGDLVVPS
                     QFANAANAILVNMVSGYYRMYKSAPSSSAILDMLKRAKRDKTIREELFADVVAAFKRI
                     LAEKLSDTSYMVDQVSTFAKSVAFDDALIKAAELKEKGDFQGAMAIMAKVQQIGSNEA
                     TGIYDYYTSASERLKAREYEASEEYVPNSITTGLPLLDRLLYQKGWAKREMVLFMGFA
                     KSGKSTAMGEFSINATLAGYNVLYLSLEVHTTILSDRFDARLSETEMSKLVERRDQVH
                     RKLAELGATKGIGSLWVVERPSGSMSPADLDRMLNSMKANGMVPDMVVVDYADLMRAS
                     YDLRDDRANIRSIYTDLRALYDKHNVAGITASQTNREGGASEVATMMHAADNIEKVRI
                     ADLVITINKTEEEEAKGEARLYFAGSRNQQGGISIRVKQNLEQMRFIERILDVT"
     gene            2689..3804
                     /locus_tag="ACHUPM_000220"
     CDS             2689..3804
                     /locus_tag="ACHUPM_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR0868122.1"
                     /translation="MSELKELIAELDFEQWLDTEGIVYRRGGVSSRGREVNIKECPVC
                     GSSNWKVYFNLTSGVGKCFAGDHPEEIQFNKLVFLKHYSGKSRRQFEEYVQNALLSQG
                     WAPKKEELVLASTVELEGPVALPRHYELPIDGRLPDYLVERNISPEMAKYFDLRYCVE
                     GKHAYVDPYTDQVKGQIFDMRILIPVYDLDGVMKTFQGRDITGTAERRYLFPMQLPAS
                     GKFLYNGHNAVGKQTVVVCEGAFDVMGVKRAIFDEETLRDYVEPIGTFGMHLSGNTTQ
                     DAEDQLGAFLTLKARGLRNVIMMWDSEKQAIRNTMAAARRLTSIGLNVKVACLGEEGL
                     DPGGATPEQIIKAYYRAKPYTKQLELQSKVLGIKALS"
     gene            3880..4662
                     /locus_tag="ACHUPM_000221"
     CDS             3880..4662
                     /locus_tag="ACHUPM_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868123.1"
                     /translation="MKDEIQKLACDIIDKTGLEISESNRLDIIEKAVKTAMDHIATRL
                     VEIPLPGLPYLKVKLHVWGEPSCARRSALVVFISKENPISLKVQVGAWMDGKVIYTNT
                     VFCASNDSIIEEAIQESLLTMHSLVLLEDKQNYEEYLRSIKGERTLSLKADFVTPTNL
                     LEVLLNKGANDAVNVIRESEYASLYDMCKSQLDLVHIVIDAGKACDGVMAEFAGKVVR
                     IANELPMIEQEAKSYATNHVTELLAPYRLESNQRKMISWGSW"
     gene            4724..5200
                     /locus_tag="ACHUPM_000222"
     CDS             4724..5200
                     /locus_tag="ACHUPM_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868124.1"
                     /translation="MVVKADLSKIPSISGNNGYSLRCEEVKINGESAYCSYSVCQHTI
                     LAFKENRLPRTSFQSCATAIKAGKCKALKMMVEEIRKGESLYFEDMTALIKEVEERNK
                     QARTIKRKRDSVTINSMVKKSATSQTAITDVYAALLEETTKETHEQIDQHMEAKQQ"
     gene            5197..6519
                     /locus_tag="ACHUPM_000223"
     CDS             5197..6519
                     /locus_tag="ACHUPM_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFR0868125.1"
                     /translation="MKKLIALKHKLDEMKAMGTNAKKEALANMDDFEQSMVSLMLNPF
                     IRFGVKKYKVAEPLSESVPSDEKAIDVLNKLASRELTGNAAIVAVESIVASMCADGQD
                     VFRRFLLKDPKAGVGISLCNKVFENPIPKFEVQLASPYKEKGDKYPFKPNPKAKWPMI
                     GSLKLDGLRVICEVIVDEEEVNFLSRTGNPITSLDHLKPAMLELGKLSGHKHIFFDGE
                     GTAGSFNQSVSALRKKNVQAIGAIYHVFDFFLPEWRAQAKSKEYAKTGMKLKERLAML
                     VALFKNDRSEGYTQDIHLHPFYIIHSHEDFIERFMKRLDDNEEGEMGKDPNSVYEFKR
                     TRSWWKLKDEDSEDGEIIDFEPGDPDSGFANTLGKIVIRLENGVIVRASGIKHKYLDE
                     IWNNKEKYRGRIVEVHCHEKTPDGSLRHPRLKWPRCLRDTEDRIGDKE"
     gene            6519..6695
                     /locus_tag="ACHUPM_000224"
     CDS             6519..6695
                     /locus_tag="ACHUPM_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868126.1"
                     /translation="MLGWMIAFLAVGFLIGIVVMSSCINDYIKNGVIERRGRIYRIVE
                     ITNTVKEIKDDRIK"
     gene            6679..6894
                     /locus_tag="ACHUPM_000225"
     CDS             6679..6894
                     /locus_tag="ACHUPM_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868127.1"
                     /translation="MIVLSKREKETLYEISKWSEFPEYWKPKTRAKLERLGLVANVSE
                     TWCSANYQLTDKGKVLLQQLVESGALK"
     gene            7040..7330
                     /locus_tag="ACHUPM_000226"
     CDS             7040..7330
                     /locus_tag="ACHUPM_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type natriuretic protein"
                     /protein_id="HFR0868128.1"
                     /translation="MSNLTSWDWWLVTYFLAAGVAFAFYVGQLVVKLLLIKFASHKRI
                     DDGLWRLGTLVETRYGQLKENETITIQAKRFTATITRTPSRRVALIKKVTTE"
     gene            8107..8181
                     /locus_tag="ACHUPM_000227"
     tRNA            8107..8181
                     /locus_tag="ACHUPM_000227"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8139..8141,aa:Asn,seq:gtt)
     gene            8187..8273
                     /locus_tag="ACHUPM_000228"
     tRNA            8187..8273
                     /locus_tag="ACHUPM_000228"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8221..8223,aa:Tyr,seq:gta)
     gene            8278..8353
                     /locus_tag="ACHUPM_000229"
     tRNA            8278..8353
                     /locus_tag="ACHUPM_000229"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8311..8313,aa:Thr,seq:tgt)
     gene            8844..8930
                     /locus_tag="ACHUPM_000230"
     tRNA            8844..8930
                     /locus_tag="ACHUPM_000230"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8876..8878,aa:Ser,seq:tga)
     gene            9156..11069
                     /locus_tag="ACHUPM_000231"
     CDS             9156..11069
                     /locus_tag="ACHUPM_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR0868129.1"
                     /translation="MSFIDYDILSASSSKELREKLAEKIAEGWQPFESPNIVEPSKGG
                     PNAYNQAIVRSGDLSQLPTPVSNEDEEPDYYYVVTIAGQSNSMSYGEGLPMPKTFDRP
                     HPRVKQLARRSNIKPGGFACKYNEIIPADHCLHDVQDMTKFKHPKANDSEYGCVGQGL
                     HIGKKLLPFIPANAGILLVPCGRGGSGFTVTGSEGQYYEESGAIESSLKWGLNTPLYK
                     DIVARTKAALSKNKKNVFLGVVWMQGEADLATGKYSQHNELFLKMVEGFRKELSQYDA
                     QCVFGSAANVPWICGDTTHWWKNKYPKQYEIVYGGYKGKTSQAIYFVPFMKDENDNNV
                     LTNSPTDDPDITSIGYYGSGHRKDNKTWTSESRESHFGSWQRRGIISDRLATALLAHS
                     AKSSAFVNGVTGEIIKQEQPTQPKNDNIVTTPTPRTVISLTGAGGDVATEQGWTVTGA
                     NIQSVENGKAIALTKAVSNETWTISKATDIAQTLLENGGKLLCKFKLSGNPDPKKAKF
                     GFGIYYKTSKPVADDISFDGTGSLFLANFFLQTIGGKLNIMMHRTGNNRNVQIDSYGD
                     YDNNWHTLELQYEKGTGTVIPVLDGKAGSKFKLVKDNIALGDEAFVLTDITNNQTHDL
                     AIETLTISINNPT"
     gene            11277..12380
                     /locus_tag="ACHUPM_000232"
     CDS             11277..12380
                     /locus_tag="ACHUPM_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0868130.1"
                     /translation="MKNSFDRARAAENTSKEAIEYLERASQMQAIMISQINNNMIFSD
                     AFMLFTRLSLLITRRRPEIAVHCILIHVLPHIADVKVSDINRFMVNQLVNPLILDGKI
                     VMGRRVFSLMKQFLSWCAFQGMIDVSPLNDMSLNKVAGGAKPTPRERKLTDAEVWVFW
                     NIWDYFNVCAGTKWAARLCLVSARRPDEVLRAKKSEFNLKRGVWNQGKRNKSAREHSL
                     PLSTLMRTCIEELFEYGKDSQWLVPSNKKIGKDLPMSKVAIAQALRRILERPELMELA
                     PFTPRDLRRTARSYFPALGISQEVSRKIMNHSLEGIDRVYDRHDYMDEMRDALESFST
                     YIASIVEQPDLDEIDHKFKGDRLSTELIRLNFS"
     gene            complement(12375..12761)
                     /locus_tag="ACHUPM_000233"
     CDS             complement(12375..12761)
                     /locus_tag="ACHUPM_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFR0868131.1"
                     /translation="MSDKNIEFIESRYAAFIAGLIESSPMSQAQIARLIGYKNANNLS
                     LIKSGKIPLPIDKVRPLALALGIEPSRLMMMVLEERQPELAAFLYKEGTAPLNEDEKQ
                     VLAAYNERFGKEKGASQKVVEAIKSL"
     gene            13009..13221
                     /locus_tag="ACHUPM_000234"
     CDS             13009..13221
                     /locus_tag="ACHUPM_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868132.1"
                     /translation="MKTTISSLITLEIGHVEKLADECVADILTELPNEQIQVGMHDAT
                     AFIFLLNNKRFTLINTGSGSLAVRTC"
     gene            13322..15430
                     /locus_tag="ACHUPM_000235"
     CDS             13322..15430
                     /locus_tag="ACHUPM_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobT"
                     /protein_id="HFR0868133.1"
                     /translation="MSKKRSIKEVQDFRDSVKRVVALLSGKNIPVAERGDDAYVRYND
                     DGEPILVNIPSIPDNATPALMNAVRGFLDHEVAHILFTDIRVSNKMREKGRVPSRSLW
                     NALEDVFIERKMGQVFNGTRRNLMATQRLIIEKVFKPKASEAIAYCGKDQRALFLNFF
                     LCPVVRAWDGQAPFVDFMDEYWPVIEKPISLLKEHGIDVAVRNMSCTEDCVKVAATIA
                     KILKDTESESKGKESAPGKTPDPSDADQTDASGENNEDNGDHETPEALDSHKSIKSES
                     HSKHKHDINDSDDSDNSESSETIFDDTENDKEVSDSDASDNAASESLTADHEKRKTTE
                     DGSSDIPTPSKISLEEALEELDSIEDEVGGMTEDALSETIKSELTESSKSEYRPYNRS
                     YDFIGSIDQAEAHIKRLIKTFSDIDLGGYPISRYRIVPEGNQLFDKYIEKHLSSGVSS
                     TLAKDLERAIASRNRVQFIPGQRRGRIHGSSIYRLAMNDDRVFRKKEESKAVNACVQQ
                     VIDLSGSMSGITIQLALASAYTIADALDRINVPNIITGFTTFGSHMAAGELKAVKYEF
                     SRFESLMLPIIKNWNEKVNSREVRSRMGCVGYTFPLLNNVDGESIASLASLFSGRLED
                     RKIMLVLSDGAPWAVGRDFDAHLRSVAKQIETQTDIDLMAIGIMTDAPERFYSNHALV
                     TSVDSLGSSVVTELSRIILK"
     gene            15526..16761
                     /locus_tag="ACHUPM_000236"
     CDS             15526..16761
                     /locus_tag="ACHUPM_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0868134.1"
                     /translation="MTTTALQNEKNPSDYLVCKWCGKSFHYFKSHVANGNCEGIPESV
                     KDADPDTVLKMYTTQFPDEPTLSKKALDAIQAKRAEQKSEMAKSSGLTSSPGYTGTVE
                     YKTDLVAAHELLNVTVEELGTKRGTPLMVSVNVNTPFPEFVPEVKKGYVYGDFELIKD
                     IFMMLELGIPGYLWGHAGTGKSSLPTQLCALLNRPLIRAQHTASMEEAHVTGQILARD
                     GSTYFEPGLLALAMKHGWVYLADEYDFAFPQILGVYQPVLEGEALVIKEATPEWRRIT
                     PHERFAFIGTGNTNGSGDETGLYQGTNIQNAANFSRFGIVSNVKYMSKEAEISMLINA
                     GIVDEYAEKMVKFAGIVRDGYEEHLISQPIGPRELLLSAKIGMMRGDFVTGIERSFIN
                     KLPSASAQAAREVVQKIFG"
     gene            16942..20460
                     /gene="dnaE"
                     /locus_tag="ACHUPM_000237"
     CDS             16942..20460
                     /gene="dnaE"
                     /locus_tag="ACHUPM_000237"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR0868135.1"
                     /translation="MKALMVRTDFSLGESALKAENAVKIAREAGYTAVISADSMNIAS
                     VIPLQRAAGDDMAVICGVKLNIVDDPTYEYRAKIAKESKGCMESLERGRNYSFTALIK
                     NEQGYRDICELMTVANTREQFYFVPRLSLEQLVSTYAKGNIILLTSDIGSVFQRNDFA
                     KIISTLITAGGKDNFYSVVYPHPTPFYDQINVRAMKVASALKIEPVAFYPAYYESIDD
                     ADIKDIAHMVTNNIKIDQPHRLRIPHQRDNAVNGRRHLLEALKTFSVRMDVPVTAAMA
                     STTQDTIIDACTWRWHELPPALPKMADDEPATLMKLAVAGLRKRLTTKEFGYTPPASE
                     NRVYVERLKYEMDTLTRLGFCGYFLMVRDLMNHSRETGIPVGPGRGSSAGSLVAWCIG
                     ITNVDPIRHGLLFERFINPERLDLPDADLDFSQARRHEVIEYLNERYGEDYVAGIPNF
                     TYLGAASALRDTARIYGVESADMAVSKELKNVEDDSLPLEELREQLASLDKYATKYPD
                     AFNAACKLQSLMRGFGRHAAGMIVAGVPLTERTPVERRGDARCIAFDKRYCEAMGLIK
                     LDVLGLATLDLLDSAKRYIKENTGEDINLDAISLEDRKVLDGFAAGYTQGVFQLESGP
                     MRKLLKDLGGGIEPMSFKTVVATTALFRPGPIQSGMLDDYVSVAKGFMTPESLHPVLD
                     ELTAETNGVILYQEQTMNATRLLAGFTMAEADGVRKAIGKKDMEKMKSMGEKFIVQAQ
                     AGWIDVELEDGTTQRIHRAEHFKCEDGTLKTVEEALEYGAKLPINAVRVTASHPGLSE
                     MKAKEIWTAFEKNGAYQFNKSHSVAYSLISYQSMWLKTHYPAEFFAAALTILGEDKHQ
                     GLVKDALTYGIRVLPPDVNVSSNRIEIRTLEDGSQVLYAPFSAVKGCSENGCQAIMRA
                     REKVGGKFESLAQFEEAVEKRACNSRVRESLQKVGAFASIEPGSLPATDPERLRDQAE
                     LMGNLVIDAVKASRPFEMNPKRSAEINVLMTRMAAEMGLGDELIRPSIGIKPKIMIIL
                     DNANGNDARTGYFMENGYDDFKAKLLTAGDLRMGDLYVTGVCKKVKDKEKDYTKDEIG
                     QFTDFIREEINLVRPTYVLTCGSRATALFNNKNKPSDLVGRKEYLPDLDVTVFYGFNP
                     NILYFRPEEGERLEAILADVAETIKT"
     gene            20586..21017
                     /locus_tag="ACHUPM_000238"
     CDS             20586..21017
                     /locus_tag="ACHUPM_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868136.1"
                     /translation="MITDIYEKIMSDLEFDRDNLEEVWRRQPRLLMEYGSKLAHAERS
                     VAEAKLNLEAVEAKLYDTERKNLSMNGIKFNESVLDAKVKTNPQYLSKRQKLDEARHI
                     ADIYKHAVAAFSHRRDMIVQASKMAIVELERLGSERFITPR"
     gene            21135..22163
                     /locus_tag="ACHUPM_000239"
     CDS             21135..22163
                     /locus_tag="ACHUPM_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFR0868137.1"
                     /translation="MSKTLLDLLNKTREDIAAKRGNNVDLTRLKDGVNYIRIFPNKDD
                     PNGKFFQTFGMHYVKYQNEEGKEATNAYICEQHTHGRACQLCEMVMEGRARHKGNKAM
                     EERIGQMRATPRYLVNGILSAREDFADAEKCQLIELPSTVFDDICKAITEDIADDIGN
                     PLSKEEGYAFLIKRTGSGRDTKYDVSPKRKVYKGDIEDKFWNTQHDLIAYANQADETR
                     LLSTVRTMGRLIGIAAPTASASAPAISSTAKTSAAALPGFGSVTGHTEGATAVATAHT
                     PASEPTSLVDEEILRAVETEFKPEASSAAVAVSVKESEAVAATSVAAASATEDEGLDD
                     LLRELDSL"
     gene            22224..23168
                     /locus_tag="ACHUPM_000240"
     CDS             22224..23168
                     /locus_tag="ACHUPM_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HFR0868138.1"
                     /translation="MNYLFVDGNSLGYYHQQSDKLHNGEMEVQAAFGFVKNVRRYASI
                     LHARPMILWDGFSDKRRDFYPDYKANRDDDPDMKKMKEGFAIQKPYILKMMTALGVTQ
                     LIAKDAEADDLAGLLVASIAPQPIVEHIYLLTGDSDWLQLVRENVSWVSLREDAKNKQ
                     VNFEQFAELTGFATPRAFLEAKALQGDKSDNISGVGGIGAGGAKELLHEWGSVATMVR
                     GINDGSIVVDKGRHKTAFNKLAKNAFNEKTGCRMLEAFKRNITLMNLIETKFPPTEIE
                     TIKGNRDVKAFEQLCYELNFRSFLEDLEVFVLPFERYC"
     gene            23168..23434
                     /locus_tag="ACHUPM_000241"
     CDS             23168..23434
                     /locus_tag="ACHUPM_000241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868139.1"
                     /translation="MLKSIINGATTTPTQLAKEIVFYHGEYAVIALPSILGAAGMKAT
                     DREFGLVSEQVVKILARVSRLLNHDAIVFDESAALKRINETKGA"
     gene            23437..24510
                     /locus_tag="ACHUPM_000242"
     CDS             23437..24510
                     /locus_tag="ACHUPM_000242"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR0868140.1"
                     /translation="MAKGKSALALALKKKIGSNDEIQKVTHWIDTGFPPLNKAISGRY
                     DGGFPCGRIVEVFGPPSAGKTFLATAAMISAQKQDGLAVFLDHENSFDVGLAVANGLN
                     ADEDDGQWVYKQPDTFEDSVELIGTILKLVRDEELIPESAPICIVADSLASMVPNSKA
                     EKFEKMAEGTAKDKDQLNMNDNTALARATSANFPTLALWARKYNACIIFLNQVRTKIG
                     VMFGDPTTSPGGDSPKFYASVRIRLGASVMKDGKEKIGQDVGAECIKNKVAPPYGKCT
                     WKFYFDPTRGLDVIESLVEYMLEEGYLPKNASGRVEIGDKKYTKSQIVEMYREKPLAE
                     IIAALQAIDDRRAKDNPTESVEE"
     gene            24603..24803
                     /locus_tag="ACHUPM_000243"
     CDS             24603..24803
                     /locus_tag="ACHUPM_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868141.1"
                     /translation="MIKIYLLAVATGVSVALIYGLLVPSLISTKSDLAVMFGVIVGFG
                     APVIGLIAGRKFINSLIKAKGK"
     gene            24807..25637
                     /locus_tag="ACHUPM_000244"
     CDS             24807..25637
                     /locus_tag="ACHUPM_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR0868142.1"
                     /translation="MKKGLLAVALAAICTMGLTGCDRVEPGYVGIKVNKLGEDKGIGE
                     VVGVGRQWTGLNTELYVFPTFKQMKTYDEPFTFQMSDGTAIGHKIGVAYLVNRDKVTT
                     VFQTYRKGVDDITESDLRQKIADSLNRLASRMTTDSFIDGGKAQLLDNALKDIQKEMS
                     PVGIEVLSLSWVGKPDYPKTVIESINAKVTANQRTLQRQQEVEQRKAEANMLREQANG
                     EADAIRARAQAEADAIRLRGEALRQNPNVMELEAINKWNGQLPQYMTQGANTPFITVK
                     "
     gene            25733..26557
                     /locus_tag="ACHUPM_000245"
     CDS             25733..26557
                     /locus_tag="ACHUPM_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868143.1"
                     /translation="MPNHITNEIRVIGGTNKQRLDFIRAITNKKGLIDFNTICRRPKS
                     IEMEENNHIRRMASAMAGETVYDHMFGELKTPEQVELAMRESGMTRKEIRKIKEQALM
                     RLENYRRYGYYSWFDWSRANWGTKWNAYNIEMPVKRIPTRIKYGHNFRHTHVRAYAKR
                     IYKKRLARHAATGGELVIRFETAWSMPKPIYQAMARKFPHLEFVIRYADEDFGNNCGF
                     ILLRNGKWEYDYIAPANNKQSSEEKIKWRKFAFELCCPGITPQEYGLNEEYEYAGY"
     gene            26605..27345
                     /locus_tag="ACHUPM_000246"
     CDS             26605..27345
                     /locus_tag="ACHUPM_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868144.1"
                     /translation="MKRKIEEPKTTLNKHGEKIQTHPAFGLVKTSRVSTTGIRLFDSE
                     LEHRDYIEIGVYEAELSLDNERTRPQRSCNRRPLVEIRLSQAQWAAMVSSFGVGEGVP
                     CTISYKTIGDSIRVHDIAEQKSIRQKFNDQIKAATNSEIDRLNQQVSELGELVKKGRA
                     GKRDLEELYRKLASAVANLPENLCFATELMQEQMDEIVSSGKAEIEAYVAGTVMRKGL
                     AALGTESCINDDPIVSITKMESGKELLP"
     gene            27366..27755
                     /locus_tag="ACHUPM_000247"
     CDS             27366..27755
                     /locus_tag="ACHUPM_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868145.1"
                     /translation="MLLIQPGFGLKIKKGHMFGERDSKRKILSIRLPFITIHWLNRES
                     TDYWYKCARAAFNDPDWFVENHHAVRQAKRKANTTYMKAYQDAWEEHRGRYQKNIEKL
                     ESENMDLRRKLGEARRDIEAYKRLVSE"
     gene            27817..28242
                     /locus_tag="ACHUPM_000248"
     CDS             27817..28242
                     /locus_tag="ACHUPM_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868146.1"
                     /translation="MAAIKKLYDAANVALDVIDDEVAKGFPEPDWAHQLRNAIAEMTP
                     PDPTPDETDWQRFIRMYAQEIGPTPTAEQAMLLKYFKEAGEDLPIDDSAYWFHCAWRK
                     YDVIFTQGMGSKDMVVWHLLHIDTAVDRVIEQFFPNQED"
     gene            28298..28573
                     /locus_tag="ACHUPM_000249"
     CDS             28298..28573
                     /locus_tag="ACHUPM_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868147.1"
                     /translation="MRVLVRIVTSTVYDVFPLFMVKVDGLNDEETDALIQRTLVEYTG
                     HDADSVMVDDDGVCWHNGNCWYVEETQQISNEDAEHLERILSISTFE"
     gene            28789..29124
                     /locus_tag="ACHUPM_000250"
     CDS             28789..29124
                     /locus_tag="ACHUPM_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868148.1"
                     /translation="MKTIPFALLFLSSIVVADTTVYQCEMSVADVKNGALTDVIKAPY
                     GAMVVDSGDQFYVVRDDRVLSSPYLTNRNGKLTGVGEDHFVYNKYKGFYGVHASQQSY
                     LFDDCKEVG"
     gene            29124..29336
                     /locus_tag="ACHUPM_000251"
     CDS             29124..29336
                     /locus_tag="ACHUPM_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868149.1"
                     /translation="MALTLAGLEIEKTSGYWRAKGFKQPGILERLEREDGYIVHQRRE
                     WRMYDPETGKLTTKAGTLWGLLKKIH"
     gene            complement(29916..31130)
                     /locus_tag="ACHUPM_000252"
     CDS             complement(29916..31130)
                     /locus_tag="ACHUPM_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFR0868150.1"
                     /translation="MLSAVIDSLSAVHRMLPTVFGILSAANQVCCQRLNDKGVHMSTK
                     NKKGKNNKEVEDNIDNFEEGSLELYTGELVPNSNNTVQPIALMRLGLFVPTLKGTKYS
                     KRNKPNEIDASKELVQLEVARSEGYSDIKITGPRLDMDHDFKTWVGVVRSLAEYGEPN
                     GRVELSITKFAKFCGYPSSQIRKTLRDRLTNSLLKIMRTTLSFQRTYEEKNVDGSNKI
                     SLLMVHLINSVDYNEQKDTVVFYAEPKLAELYRFDHKVLLQLKVINKLPRKETAQALY
                     TFIESLPTKPAPVSLARLRARLNLSSRNVSSQNQTIRNGLKALQDLGYLEYSEIKRGR
                     SIYIQIHSRNPKLKVAPPKPEDIEPKKPDEKAGEIDAKQNIINKITELSQNLTPENIK
                     MIEILSNSLKLL"
     gene            31332..31976
                     /locus_tag="ACHUPM_000253"
     CDS             31332..31976
                     /locus_tag="ACHUPM_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868151.1"
                     /translation="MDLKRTRWVRRLEDGSYTIESNSNLNKQKLLCDICGIAAKCPIY
                     ETRIKLDKAGVNFHLNSCIRYVPLLAFRKPIIGLDAPYFNTLRSGVTWRDRLSPDKLI
                     CLVSADTGKIIRFGKVDKVYSGPVDEMLRKHSRFNHLCMGGEKIEKVEEVIRKSYGHF
                     LTKDSLLTAIYIRHVKREFDLEYHSEEELNLVDPRPKAGVISINAARKKPTDAL"
     gene            32231..33316
                     /locus_tag="ACHUPM_000254"
     CDS             32231..33316
                     /locus_tag="ACHUPM_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFR0868152.1"
                     /translation="MTLPYGVISDCHYHKWDAFSTTNAEGLNSRLEIQLEATKEAAIA
                     MKKAGCKYMLVAGDTFHVRGTVSPSVLHYVTETYKWIINELDLTVVMLAGNHDLETND
                     SVYSANAAASLSSIGVVIVCGKRPHSIKIGDVTVHLISWRNNHAELISDLKALRKSVE
                     GDNHDVVIHTSINKAIPTMPDVGIDAQELKDIGFRLVLSGHYHNHKEVIPGVISVGAL
                     THQNWGDVGSLAGYMIVNPDGSFSHYETSAPKFINLEDDVADDQIRGNYVRFRAVIEN
                     DEEGIKYQNILKTMGAKGVVCNFIRKSSMMEGTASTTETSKIDSLGESVSAYCKIVHD
                     TDGGFDLSKLDILCQEILTEAESSEAV"
     gene            33546..35462
                     /locus_tag="ACHUPM_000255"
     CDS             33546..35462
                     /locus_tag="ACHUPM_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0868153.1"
                     /translation="MKFLKLQVENFMAIASAEVELDQRGLVLIQGVNSDDSSASSNGS
                     GKSTLMNSLMWCLYGETAHGVKGDDVLSTDHEKNCRVAVTIEDEGKRYAIIRHRKHKE
                     FKNRLIVRGEDGDMTKGKDALTQEFVERLIGASKEVFMASIYASQEAMPDLPGMSDKN
                     LKTIVEEAAGVDRLTRAYAIARERANAAAARMDVVKTKLESTISTIEATQSEIESAKA
                     SSESWEQERSKRYDDALAGLASAEVELTEVELEIRTLPEQIRDTEKAIESERKKLASK
                     EEHDAKLLKVRGAITDIRASIKATENSQADAMNRARNFKTKAEEVGTKVGSPCPTCGK
                     AYCEEDLSTVKENFIEQARQEIGQAKTLAEAMAKHKTNLEKALSIESALVKTTPDVTA
                     IIARIEELTKQLSSLRHREKEIVAIESLVTRARTEVDRISKEINPFIALIARHEDNLV
                     SSKSTFKSLKDELKAIQEQTLLLEKARQVYSPAGVRSHILTSVTPFLNTRTAEYLNTL
                     SDGNITAEWSTMDVTKKGEYRDKFNISVQKKGSSKSFQTLSGGEKRKVRIACSLALQD
                     LVSNRASKNIDLFIGDEIDDALDTAGLERLMGILESKARERGTVLIISHKEMKSWFRE
                     TITLEVKEGRSYVV"
     gene            35452..36195
                     /locus_tag="ACHUPM_000256"
     CDS             35452..36195
                     /locus_tag="ACHUPM_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868154.1"
                     /translation="MSFKLSRSQFLQVFAVMQSIKLINGHTSNGAAPRILWGSNNIDG
                     VQFAALLGLISETPLMQSLKSLPPGCIAPILINPFVEGGYLPNVGPGFIASHENEDLN
                     INSEGFFGGMGAHHCMAFTNLIRLANKRVDSLASPGDAFTGFLIQRRDKKYSADKLQF
                     VGKYGEMVEIELQLPHVLANDSADSRRLLGIMRHFIASGVKHAVDKRVTQENEYSDFA
                     NSPQPTLQTAIVTNSLEARLLENPIWGTW"
     gene            36205..36774
                     /locus_tag="ACHUPM_000257"
     CDS             36205..36774
                     /locus_tag="ACHUPM_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868155.1"
                     /translation="MSKKISVVGVDPSMSNFGLAVGTLDLETDELEIHDLTLVETKAG
                     SNKKTVRVNSDDLRRASEIWRVAKPIIDKANMVFCELPVGSQNSRSQTSYGICIGVLA
                     CVDKPLIQVTPNEIKHFVGNKLTTSKEEIIQWATKKHSKAPWLRRKQSGQDVLVNKNE
                     HLADAVAAIHTGMQTDQFRQVRDVLKSLI"
     gene            36848..39163
                     /gene="nrdA"
                     /locus_tag="ACHUPM_000258"
     CDS             36848..39163
                     /gene="nrdA"
                     /locus_tag="ACHUPM_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0868156.1"
                     /translation="MISIVKRNGQTEPLSEEKYNRVVMYGVEGIRGVSASAVAMGAAA
                     SIFDGMTTSQLHEALVKSAADLISPEAPNYSQVAARLNIFKIRKDAFGRYDYPNFYQH
                     IVKNVNKGVYDKDLLTHYSFEEIEELGNYIKPKRDDLFGYAATVQLQSKYLVQNRVTG
                     EIHEGPQHIYMLVGMCLFQNWEDDCAGKTRMEMVKGFYDVTSTFKLSLPTPIMAGVRT
                     PTRQFSSCVLIESGDSLKGISAASAAIIDYVSRRAGIGIGFGRIRALGSEIRNGEATH
                     TGVIPFLKHFQTAVKSCSQGGVRGGAATAFYPIWHLEVESLLVVKNNRGIDENRVRHL
                     DYGVMSNRLMYRRLVRSENITLFSPHDVPDMYEAFFTDQELFEKLYHKYEADDSIRKK
                     SVPAIELFSSLMQERASTGRIYIANVDHINEHGAFIPALAPVRQSNLCMEITLPTRPL
                     AFTDDPNGEIALCTLSAFNLGAIRSLESLKEVAFYAVAALDSLLDYQDYPMEAAEVPA
                     KARRSLGIGVTNFAYYLAKNGIRYSDTAGNKLVHETFEAIQYYLLDASCQLAEAKGEC
                     DWFEQTKYAIGQLPIDHYRSSLDESGETNFELKMPWEELRERIAKYGLRNSTLTAQMP
                     CETSSQITNSTNGIEPPRGPVSVKSSKDGIVKMVVPEIEKLKEQYEYLWDMPDNRGYL
                     TKVAIIQKFFDQAISANTNYDPSRFEGDKVPMMTLLSDLLLAYKMGVKTLYYHNTRDG
                     AGKRDDDEPQNPLTQAVAVEPEDECDGACKI"
     gene            39269..40411
                     /gene="nrdB"
                     /locus_tag="ACHUPM_000259"
     CDS             39269..40411
                     /gene="nrdB"
                     /locus_tag="ACHUPM_000259"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0868157.1"
                     /translation="MSYSTFRLGANDATKEPMFLGQSVNVARYDQQKYRDFEKLIERQ
                     LSFFWRPEEVDISSDRIDFNTKLRDHERHIFLSNLRYQTLLDSVQGRSPNATLLPLIS
                     IPELETWVETWSFSETIHSRSYTHIIRGMVDDPSIVFDGIVTDEEIINRAISISAEYD
                     RLYGMTCERQSLGEKEFERLYVNEYGWEPYPLHRQLFRTLVSINALEAIRFYVSFACT
                     FAFGERKLLEGNTKIMRFIARDEALHCEGTERMIRFMRTGREGLLWKEIAADEENVIY
                     DTMKSVAEQEMNWAEYLFKDGSMIGLNADILKTYVKYRTNLAMNRLGLKALFPEVTTD
                     PLVWMNKWLLTDTLQIAPQEAEQSTYLVGQIDSTVDKASLSQFADL"
     gene            40489..41358
                     /locus_tag="ACHUPM_000260"
     CDS             40489..41358
                     /locus_tag="ACHUPM_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphohydrolase"
                     /protein_id="HFR0868158.1"
                     /translation="MNFTKLTDHLKLATDRLIGFKPEPYELHEGHGVATESIYKMVDQ
                     FHELFQHPRRVMPTPELLRLRASLIHEEAVVEGIPAAMNGDIEQLLDAMADFLYVGVG
                     TMVAIKGGISTGMTYYTQEQSIDRFMQTIFVPGNTVFDDMAMPFQEAREASCMLEELA
                     DKLENKTVKDSELIQELRRVMNKIYVACMMTYRLADFLGINVVELVGEIHRSNMTKLW
                     PADVEERRQAVANCKYDSSDLGFRHADGTDKMIGFRISDGKILKSPTYSDVDLSSFVE
                     QAKASAMYGMIKK"
     gene            41526..42629
                     /locus_tag="ACHUPM_000261"
     CDS             41526..42629
                     /locus_tag="ACHUPM_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR0868159.1"
                     /translation="MSLLLNREHTNGQVTNASYAKVIETVLKSGVQTDDRTGTGTLST
                     CYVPSYYMLTGGTVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWADE
                     NGDLGPVYGKQWRRWEDTRIVSHSEYLSKIATFRERGYKVEGYLGISEDRVVLSREID
                     QLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMATD
                     IGLQHSRFGYESIYTKMLYDLEMDGSVTETELDELGIPKRILNSCLVQRSVDTFVGMP
                     FNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMKRQAPEHSDPVVI
                     FPHEWSELDDFKWDDILILGYDPLPWIKVPVAV"
     gene            42631..43044
                     /locus_tag="ACHUPM_000262"
     CDS             42631..43044
                     /locus_tag="ACHUPM_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868160.1"
                     /translation="MARGMYVLCEIEGVLANTSHRKSVPDTDAGQLIAGDELIFPTSR
                     MLRGFARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNGTSYEKHIKRTLA
                     EHKGDLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            43041..43517
                     /locus_tag="ACHUPM_000263"
     CDS             43041..43517
                     /locus_tag="ACHUPM_000263"
                     /inference="COORDINATES: protein motif:HMM:NF047324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFR0868161.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETVFTQRASEEEPGFSQIVYRRS"
     gene            43517..44161
                     /locus_tag="ACHUPM_000264"
     CDS             43517..44161
                     /locus_tag="ACHUPM_000264"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0868162.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGESMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            44223..44642
                     /locus_tag="ACHUPM_000265"
     CDS             44223..44642
                     /locus_tag="ACHUPM_000265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868163.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGAQVGEAINIAMSQFSEDSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TASGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            44652..45209
                     /locus_tag="ACHUPM_000266"
     CDS             44652..45209
                     /locus_tag="ACHUPM_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFR0868164.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMEACSAWVAHNGEAFDIPF
                     IRHEFSGYGVRLPDVPVIDTMLSGLWATEDGKRPRLEELAFSLGFIYDHAKAHSALYD
                     TNLMMQCFFKARNKYGFFKLPSEIV"
     gene            45370..46179
                     /locus_tag="ACHUPM_000267"
     CDS             45370..46179
                     /locus_tag="ACHUPM_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868165.1"
                     /translation="MSSVENVMTNDDLDELTAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GIDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVINAESGEAAENEPEQL
                     GIIEVEDCVEVNDELKVQQSNDSNTNKKTRAARGPSFTLSDKDDSFFNKAGLEKDIFL
                     DAYENAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            46362..46955
                     /locus_tag="ACHUPM_000268"
     CDS             46362..46955
                     /locus_tag="ACHUPM_000268"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFR0868166.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPNFIGIERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATEYANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKIPLDVMKSLGGYDPANVIRKKRPSKKAA"
     gene            47142..47372
                     /locus_tag="ACHUPM_000269"
     CDS             47142..47372
                     /locus_tag="ACHUPM_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868167.1"
                     /translation="MSYPTNVVALVDSDFLAQAREMMKDREQAFNLYEWAIKCLHLGE
                     HRELVEQLLGELINEVFALNVQLHGRENNQSK"
BASE COUNT        13811 a        10333 c        11397 g        12323 t
ORIGIN      
        1 atagtgacat agcatatctt aacagtttct ctgaagatga ttatttaaag tggatattat
       61 cccgcaatca ggatgctttg gatttagtga gaaaggggtt gctcagattc aaaagtatgc
      121 aagaactaac agaagaacag caaaaaatca cgaataaagc aataggtgca ttaacgaaac
      181 tggcctctag atcaagtctt aacaaactac gtgtgagcag attgcttaat aattaagtat
      241 tcatgacctg tttctgtata tataaataat aagtaactta ttaaatatac ggaagcaggt
      301 ctttcaaaag atacccacca gaacaactcc cttccgtttc cacttccaaa aactgccacc
      361 aatcgctatc atcagctcat tgtgataagt aagtaactac ctatcaggtg agccacatga
      421 gccaaatctt tttcgacacc atcgacaacg accagtacga cttcatgaca gagtggaata
      481 ccgctgttat ggataagtgg gttgctgaaa acattggttt gtcgcgctgc aaagacgagg
      541 ctgaactctt cgatacgaag tggtttgatt accgcgacat gcatcctctt atggccacct
      601 gtctgtttac ggaggcatac aaacgtcagt actcaaatat catgctgacg cacggtcgcg
      661 aacactttga aacagctccg ttcaccaccg ggttaaaacg cctgccttat caggagttgt
      721 cgactgccaa taaaacgtcg ctatggaaag cacgccagtt tgctgaccgc tattgttgct
      781 catacgacta ctttatctcc accgtgcttt ccgcagctgc acgacgtttg tgggacaagc
      841 tgcctcgccc acagcatttg tggcagccag aactgattga gatatttgaa gagaaattag
      901 ccagacgcgc aacaacccgt ctggatgact ctctggttag ctttaagcat atgggagaca
      961 tgcagttcaa cccgattcag gaaagctatt ttgagtggat tctggagcgt ttacgcgcca
     1021 tcccccgcag caagcgcata cgcgcaattt tctccgctat ctggctaatg gaaatcgttc
     1081 cagagcgcct tatttccgcc cactttccag aagaactgga agaagcacgg tggtttattg
     1141 atcccctata taattaacta atactagaaa acaatttgtt taaaaaacaa aggaaagcac
     1201 atgaccgaac tttgccatac aggacgcggg ctgtccgaag agtttgatga agatttccag
     1261 aacagactga cggcatattt ttgtcgtgat cacgagtttc ttactcgtgc gggagatctg
     1321 gttgtgccta gccaatttgc caatgcggcc aatgccatat tggttaatat ggtttcgggc
     1381 tattaccgta tgtacaagag cgcgccctct tcatctgcaa ttctggatat gcttaagcgt
     1441 gcgaaacgcg ataagactat ccgtgaggaa ctattcgccg atgttgttgc tgcgtttaag
     1501 cgcattcttg ctgaaaaatt gtccgatacc tcgtacatgg ttgaccaggt atcaaccttc
     1561 gcaaaaagtg tagcgtttga tgatgctctg attaaggctg ctgaactgaa agagaagggc
     1621 gactttcagg gggcgatggc aatcatggcc aaggttcagc agattggatc gaacgaagcg
     1681 accggaatct atgactacta cacctccgca agtgagcgat tgaaagcgcg tgaatatgag
     1741 gcttcagagg agtatgtgcc aaacagcatt acaactggac tccctctgct cgataggttg
     1801 ctgtaccaaa aaggctgggc gaagcgtgaa atggtgctct tcatggggtt cgctaaatcc
     1861 ggtaaatcga ccgcaatggg tgagttttcc ataaacgcaa cgcttgctgg ctacaatgtt
     1921 ctgtatctct cgctggaggt tcacaccacc attttatctg accgttttga tgcaagattg
     1981 tcggagacgg aaatgtccaa gctggtggaa cggcgcgatc aggttcaccg taagttggca
     2041 gagttgggag ccacgaaggg gattggtagt ttgtgggtgg ttgagcgtcc gtcaggaagt
     2101 atgtcaccgg cagatctgga ccgtatgctt aacagcatga aagccaacgg catggtgcct
     2161 gacatggttg ttgtcgacta cgcagatttg atgcgtgcca gttatgacct tcgtgatgat
     2221 cgcgccaaca ttcgtagtat ctacaccgat ttacgtgctc tttatgacaa gcataacgtt
     2281 gctgggatca cggcatcgca gacaaaccgt gaaggtggcg cgtcagaagt tgccacaatg
     2341 atgcacgctg ccgacaacat cgaaaaagta cgtattgctg acctggtaat aacgatcaac
     2401 aaaaccgaag aagaagaagc gaaaggagag gctcgtctct actttgctgg ttcacgtaac
     2461 cagcagggag ggatcagcat tcgcgttaaa caaaacctcg aacaaatgcg cttcattgag
     2521 cgaatcttag acgttaccta aaaaataagc gtggagaaca cctccacgct tgattcattg
     2581 gtgaaacaac ttttcttttg ccaaaccaca aaagaaaaac acatgagcct ttatgttata
     2641 tcaacattta tgttggtcat aatattgcct gttaaaagtg gaattatcgt gagcgagctg
     2701 aaagagctaa ttgccgaatt agattttgaa caatggttgg atactgaagg tatcgtttat
     2761 cgacgtggag gtgtgagttc tcgcggtcgt gaagtgaata tcaaagagtg tccggtatgc
     2821 ggcagctcca actggaaggt atatttcaat ctgaccagtg gcgtcggcaa atgcttcgct
     2881 ggtgatcatc ccgaagagat tcagttcaat aagctggtct tcctcaagca ctacagcggt
     2941 aaatcacgac gacagttcga ggaatatgtg cagaacgctc ttctttccca ggggtgggca
     3001 ccaaagaaag aagagctagt gcttgcaagc acagtcgagt tagaggggcc agttgcactc
     3061 cctcgtcatt acgagcttcc tatagatggc cgtcttccag actatctggt tgaacgaaac
     3121 atatcgcctg aaatggcaaa gtattttgac ctacgatact gcgtcgaagg caagcacgct
     3181 tatgtagatc cgtatacaga tcaggtaaaa gggcagatat tcgatatgcg aatactgata
     3241 ccggtttacg atctggatgg ggtaatgaag acatttcagg gacgagacat taccggtaca
     3301 gcagaacgcc gctacctctt tcctatgcag cttccagctt caggtaaatt tctctacaac
     3361 ggccataatg cagtcggcaa acagactgta gttgtctgtg agggtgcgtt cgatgttatg
     3421 ggggtcaaac gagctatttt cgacgaagaa acattacgtg attacgtgga gccaatagga
     3481 acgttcggga tgcatctatc tggcaacacc actcaggatg cagaagatca gttgggcgcg
     3541 ttcctgacgc tcaaggcgcg tgggttacgt aatgtgatca tgatgtggga tagtgaaaag
     3601 caagctatac gcaacactat ggccgcagct aggcgactga ccagtatcgg tcttaatgtc
     3661 aaagttgcgt gtttgggtga ggaaggactc gacccgggcg gtgcaacgcc agaacaaatt
     3721 atcaaagcct attatcgggc aaaaccgtat accaagcagt tggagttaca aagcaaggtt
     3781 ttgggcatta aggctctatc ataacaatca cctttaaaat aagtagatga ttacttatct
     3841 ttctgtgaga atactttcat ctgttagcta ggagttggta tgaaagacga aattcagaaa
     3901 ttagcctgcg acatcattga taaaactggt ttagaaatca gcgagagcaa tcggctagac
     3961 atcattgaaa aagccgtaaa aacagcaatg gatcatatcg ccactcgttt ggtcgagatc
     4021 ccgctaccgg ggctacctta tctgaaggtt aagttacacg tatggggtga accttcttgt
     4081 gcacggcgtt ctgcattagt tgtttttatt agcaaagaaa acccgatcag tcttaaagtg
     4141 caggttgggg catggatgga tggcaaagtg atctacacaa ataccgtttt ttgtgcgtca
     4201 aatgacagta ttattgaaga ggctattcaa gaatcacttc taactatgca tagcttagtt
     4261 ttgctggaag acaagcaaaa ctacgaagag tacttacggt cgataaaagg tgagagaaca
     4321 ttgtcactta aagccgattt cgttaccccg acaaacctgt tggaagtctt gcttaataaa
     4381 ggggctaatg atgccgtaaa tgtaatcaga gagagtgagt atgcgtctct ttacgacatg
     4441 tgcaaaagcc agttggatct ggtgcatatc gttattgatg ctgggaaggc atgtgatggc
     4501 gtaatggcgg aatttgctgg gaaggtggtc aggattgcta acgaattacc gatgatagag
     4561 caagaggcta aatcatacgc caccaatcat gtcacagagc ttcttgcccc ctatcgctta
     4621 gaaagcaatc agcgcaagat gattagctgg ggaagttggt aatctctcag tgtgtcgttt
     4681 tttacgcaaa gaatgatagg taagtacaag attatttgtg gcggtggttg tgaaagctga
     4741 tttgtcaaaa atcccctcta tttcaggaaa taatggttat tcacttcgtt gtgaggaagt
     4801 aaagataaac ggtgagtcgg catattgcag ctactccgtt tgccagcaca ccattcttgc
     4861 cttcaaagaa aaccgtcttc ctcgaacttc attccagtcg tgcgcaaccg ctatcaaagc
     4921 aggcaaatgc aaggcgttaa aaatgatggt tgaagagatt cgtaaaggag aatctctgta
     4981 tttcgaagat atgaccgcac tcattaagga ggttgaagaa cggaataaac aagccagaac
     5041 tataaaacga aagcgtgaca gtgtaacgat taatagcatg gttaagaaga gcgccacatc
     5101 acaaacagcg atcactgacg tgtatgcggc gttgcttgaa gaaacaacaa aagaaacaca
     5161 tgagcaaatc gatcaacata tggaggcaaa acaacaatga aaaagttgat cgcacttaaa
     5221 cataagctgg acgaaatgaa ggctatggga accaatgcaa aaaaagaggc attggccaac
     5281 atggatgact ttgaacaaag catggtttca ttgatgctca accctttcat ccgttttggg
     5341 gtaaagaaat acaaagtggc agagccgctt agtgagtccg tcccaagtga cgaaaaagcc
     5401 attgatgtac tgaataagct ggcctctcgc gagctgacgg ggaacgcagc aatagttgct
     5461 gttgagtcta tcgtggcgtc aatgtgcgcc gatgggcagg acgtcttccg tcgtttcctc
     5521 ttaaaagatc cgaaagctgg cgttgggatt agcctatgta acaaggtttt tgaaaatccc
     5581 attccgaaat tcgaggtgca gctggcgtca ccgtataaag aaaaaggcga caaatacccc
     5641 ttcaagccaa atcctaaagc caaatggccg atgattggca gccttaagct cgatggtttg
     5701 cgagtaattt gtgaggttat tgttgacgag gaggaggtta acttcctttc tcgtactggt
     5761 aatccaatca cgtctctcga tcacctaaag ccagccatgc tcgaattagg caaactttca
     5821 ggccacaaac acatcttctt cgatggtgaa ggaacagccg gttcatttaa ccagtccgta
     5881 tctgcattgc gcaaaaagaa cgtgcaggca attggcgcta tttatcatgt tttcgacttc
     5941 ttcctaccgg aatggcgagc acaggctaaa tccaaagagt atgcaaagac aggtatgaag
     6001 ctgaaagagc gcctggctat gctggtggcg ttgttcaaaa acgatcgcag tgaaggctac
     6061 acacaagaca ttcacctgca tccgttctat atcatccata gccacgaaga cttcatcgaa
     6121 cgcttcatga aacgcctgga cgataacgaa gaaggggaga tgggcaaaga tccgaactct
     6181 gtttacgagt ttaaacgtac ccgcagctgg tggaagttaa aagacgaaga ttcagaagat
     6241 ggtgaaatta tcgactttga gccgggcgac ccggactctg gttttgccaa cacacttgga
     6301 aaaattgtta ttcgtcttga aaacggcgtc atcgttcgtg cgagcggcat taagcataaa
     6361 tatctggatg agatctggaa caacaaagag aagtaccgtg gtcgtattgt cgaggttcat
     6421 tgtcacgaga aaacaccgga cggcagctta cgccacccac gactgaaatg gccgcgttgc
     6481 ttacgcgata cagaagatcg aatcggagat aaagaatgat gctcggctgg atgattgcat
     6541 ttttagcggt tggtttttta atcggtattg tggtgatgtc cagttgcatt aatgactaca
     6601 ttaaaaacgg tgttatagaa agacgcggtc gcatttatcg cattgtagaa ataaccaaca
     6661 ccgtgaagga gattaaggat gatcgtatta agtaaacggg agaaggaaac actttatgaa
     6721 atcagtaagt ggtcggagtt ccctgagtac tggaagccta aaacgcgggc taagttagag
     6781 cggttagggt tggttgcaaa cgtttctgaa acgtggtgtt cggccaacta ccagttaact
     6841 gataaaggga aagtattgct acagcaatta gtagaatcag gagcgttaaa atgattccat
     6901 acatctcatt agcttttatg ggtggcttcc ttatcggctt cggcatctgt cgtgatttaa
     6961 ttaagcagga acttaaaacc aaaacactgt gcatcggaaa gcgtgtgtat cgggtagttc
     7021 atgaaacaaa ggttagaaaa tgagcaattt aacttcttgg gactggtggt tggtcaccta
     7081 tttcttagcg gccggagtcg cattcgcctt ttacgtaggt cagttagtcg taaaactgtt
     7141 gctgattaaa tttgctagtc ataaacgtat cgatgatggt ctgtggcgtc ttggcacctt
     7201 ggtggaaact cgctacgggc aacttaagga gaacgaaacc attactatcc aagcgaaacg
     7261 attcactgcc accatcacaa gaacacctag tcgtagagtg gccttgatca aaaaagtcac
     7321 aaccgaataa aaacacaata ataagtgttt acttacttat tttctatgta taagatgact
     7381 ttgttttcgt tgagacgcga ctgtttgaac ttaaatacaa gtgcaaacga agaagtctat
     7441 ctggcagtag cctaataagc caaacaccag cgaggtcagt ttccagcctc gttaccgaaa
     7501 tgggacacac tgagcgagtg tgattgcaga acgcaggagg gaacatgagt gttccctccg
     7561 atgaagtaac agaatgggcg gtcggtatat tttcaactcc atatgactcc cggattctta
     7621 gccactgacc gcccattctg ttacgtcatt ttgttcaatt atgtcgttta tactgggtta
     7681 aaaagcggcg acgtagcccg gctggtatgg ttagccagca cacaacgttg aggccattac
     7741 atttttatca attctaaggt tctattcaca gagataccgg cgagcgttga tatgtaacat
     7801 gttgggcaaa cattcaatcg gagtggtggc ctcaacgttg tgaagacagg attgttgtgt
     7861 aggtttaacc actgttgcca ttggtgtctg ttttcacaac aaatgattcc atacattaca
     7921 ttgtataaat tacaaagtag gtgctgtcct cagaaacatc atctacttaa agatttagcc
     7981 ttctattgag cgaagtcgaa agcgtctggc actaacgaag agtgcaagta gcggtgcgtt
     8041 tcctggcaga aactaacacc gccgcgattg gcactgttga gtaataaata ctgtcagtgc
     8101 tgaattgatg gtgtagctca gcggtagagc agttggctgt taaccaactg gtcggtggtt
     8161 cgaatccacc caccatcgcc aatttagggg agttagtccg tagaggtagc ggggtagact
     8221 gtaaatctac tgtcattgcg actcgggtgg ttcgactcca tcactcccca ccaaattgcc
     8281 gatttagctc agttggtaga gcagtcgctt tgtaagcgaa tggtcatcgg ttcgatcccg
     8341 ttaatcggca ccaacataat attgagaaca ttggcgtaac ggggtcatat cccaatctat
     8401 gaataaatgt tgcgttgccg cgtgacaacc agtgttctca acattgtggt gaatgcacag
     8461 gctgatgtgc cgcaactaca gtagtgcgcg ctttgcgggg cttgctacaa ccctgtgtcg
     8521 gagatcagcg ccgaccatca caatttggct ctcttgcaac agcataacgc tgaaatatgt
     8581 ccaacctggt gcggcctgtt cacccgccgt tagctccacg aaacggagcg cacaacaggt
     8641 aagaacattt tcagagttcg actctctgag ttgccattcg tggtggcacg cgagacgtga
     8701 atgctctttc cgttgtggtt gatgcgcaga ctgatgcgcg gaaagaaatg ccccagcgac
     8761 acacgaggcg agttcacaca ctgcaatgca gtcagtagcc ggggtcgcta agccggagat
     8821 cagtaccggt aaccacaacg actggagagt agggagcatg gtgctcaagc ggtcttgaaa
     8881 accgtcccat tgcgcaagcg atgatggttc gattccatta ctctccgcca gacacagcgt
     8941 tgagcggttt ggccttttaa tcaactcgat taagactccg ctaacataaa ccagaccgct
     9001 caacgctgtg tgtgaaattc cacatatttt acacatatcc atttacgcaa tttttattgt
     9061 tctttcatac attactctta tattttattg tcgtttttta actgctaaaa tcgatacgat
     9121 tttagctaaa gtttatcgtt aaaggagtaa cgattatgtc attcattgac tatgatattt
     9181 tgtcagcatc atcaagcaaa gagcttagag aaaaacttgc ggagaaaatc gcagagggtt
     9241 ggcaaccttt cgagtcacca aacattgttg aaccaagtaa gggcggacca aatgcgtaca
     9301 atcaagcaat tgtgcgatct ggtgatttat cacaattgcc cacaccggta tcaaatgaag
     9361 atgaagagcc tgactattat tatgtagtta ctatcgcagg tcaatccaac tcaatgtcat
     9421 acggcgaagg actgcctatg ccaaaaactt ttgatcggcc acatcctcgc gtaaaacagt
     9481 tagctcgacg cagcaatatt aaaccaggtg gttttgcctg taagtacaat gaaataattc
     9541 ccgctgatca ctgtcttcat gatgtacaag acatgactaa attcaagcat ccaaaagcaa
     9601 acgactctga atatggatgt gttggtcagg gactacacat tggcaaaaaa ctattaccat
     9661 tcattccagc aaacgcaggt attttgctcg ttccttgcgg aagaggtggt tctggtttca
     9721 ctgttacagg ctctgaaggc caatattatg aagagtcagg tgcaatcgaa tcatctttaa
     9781 aatggggatt aaatactcct ttgtataagg atattgtagc cagaacaaaa gcggccttaa
     9841 gtaaaaacaa aaagaatgta tttcttggtg ttgtctggat gcaaggcgaa gctgatttgg
     9901 caactggcaa gtactcgcag cataacgaac tgttcctcaa gatggtcgaa ggtttcagaa
     9961 aagagctatc tcaatacgac gcacaatgtg ttttcggaag tgctgcaaat gtaccctgga
    10021 tttgtggcga tacgacccac tggtggaaaa acaaatatcc aaaacagtac gaaattgttt
    10081 atggtggtta taagggaaaa acctcacaag caatttattt cgttccattc atgaaagatg
    10141 agaatgataa taacgtatta accaatagcc ctacagatga tccagacatc acatctattg
    10201 gctattatgg ttccggtcat cgcaaggata ataaaacatg gacttccgag agtagagaga
    10261 gtcattttgg ttcatggcag cgacgcggaa taatatctga tcgtctcgca actgcgctgc
    10321 ttgcacattc agcgaaatca tctgcttttg tgaatggcgt tacaggtgag attattaaac
    10381 aagaacaacc tactcaacct aaaaatgaca acattgtcac aactccaacc ccgcgaacgg
    10441 ttatttcctt aactggagct ggtggtgatg tcgctactga acaaggatgg actgtaactg
    10501 gtgcaaatat tcagtcagtt gaaaacggaa aagcaattgc tttaactaaa gcggtttcca
    10561 acgaaacttg gacgatcagt aaagctactg acatagcaca gactcttctt gagaatggag
    10621 gtaaattact ttgtaagttt aaattatctg gaaaccctga tccaaaaaaa gctaagtttg
    10681 gctttggtat atattacaaa accagtaaac ctgttgctga cgatatatca ttcgacggta
    10741 ctgggagcct gtttctggct aacttcttcc ttcaaacaat tggaggcaag ttgaacatta
    10801 tgatgcacag gacaggcaat aatcgaaatg ttcagataga ctcatatggt gactacgaca
    10861 acaattggca cactcttgag ttgcaatatg agaaaggcac aggaactgta attcccgttc
    10921 tcgatggcaa ggcggggagt aaattcaagc tggttaaaga caatattgct cttggtgatg
    10981 aggcttttgt tttaacagat ataacaaaca accaaactca tgatcttgct atcgaaacac
    11041 ttaccattag catcaacaac ccaacctaac acaaagtaac agccaggcat gaaattgcct
    11101 ggcttcatat cgaatcaaat atttaaaact agctctacag aaaaaaatga ttccatactc
    11161 tttaggtatg gaatcatttt ccgcatgaaa gattttagct tgattccaaa agaatcaatg
    11221 cctaatatac tggttactta tacaggtgat cagcgtaact aggtgaatgc caataaatga
    11281 aaaatagctt tgacagagca cgcgcagcag aaaacacctc aaaagaggca attgagtatc
    11341 tcgaaagagc atctcaaatg caggccatta tgatctcaca gatcaacaat aacatgatat
    11401 tctcggacgc attcatgtta ttcactcgct tatctctgct gataaccaga cgtcggccag
    11461 agatcgctgt tcattgtatt ttgatacatg ttttgccgca cattgctgat gtaaaagtaa
    11521 gtgacattaa taggttcatg gttaaccaac tggtcaaccc tctaatattg gatggcaaaa
    11581 ttgttatggg ccgccgcgtt ttctctctga tgaagcagtt ccttagctgg tgcgccttcc
    11641 aagggatgat agacgtgtca ccgttaaacg atatgtcact aaacaaagtt gccggtggcg
    11701 caaagcccac acctcgcgag cggaagctga ccgacgcaga ggtatgggtg ttctggaata
    11761 tatgggacta cttcaatgtg tgcgctggta caaaatgggc ggccaggctg tgtcttgtat
    11821 cagcaagacg acctgacgaa gtactgaggg ctaaaaaaag tgagttcaat cttaagcgtg
    11881 gagtttggaa tcaaggcaag agaaacaagt cagcacgtga gcattcgctg cctttaagca
    11941 cattaatgcg cacatgcatt gaagagttgt tcgaatacgg taaagacagc cagtggctcg
    12001 tgccttcgaa taaaaaaatc gggaaagacc ttcctatgtc taaagtggca atagcccagg
    12061 cattacgtcg tattctggaa cgaccagaac tgatggagct tgcgccattt acacctcgag
    12121 atttgcgtcg tactgcgcgt agttacttcc cagcattagg tataagccag gaggtatcac
    12181 gcaaaataat gaaccacagt cttgagggga tagatcgggt ctacgaccgg cacgattata
    12241 tggacgagat gcgagacgcc ttagaaagtt tctcaacgta catcgcatca atcgtagagc
    12301 aaccggattt agacgaaatt gaccacaaat tcaagggaga tcgtctatca acagagctta
    12361 ttcgtctaaa tttttcatag agactttatg gcctcaacaa ccttttgtga tgcgcctttc
    12421 tctttaccga atcgctcgtt atatgcagca agaacctgtt tttcgtcctc gttaagagga
    12481 gcggtgcctt ctttgtataa aaatgctgcg agttcaggtt ggcgttcttc cagcaccatc
    12541 atcataagac gacttggctc aatacccagc gccaacgcca gcggacgaac cttatcgata
    12601 ggcaaaggaa ttttaccact tttaattaaa gaaaggttgt tggcattttt ataaccaata
    12661 agtctggcta tttgggcctg actcataggt gaggattcaa tcagccctgc gataaaagca
    12721 gcatagcgac tttctataaa ttcaatgttt ttgtcagaca ttattgcgac ctttgcgcgt
    12781 taaactctct ggtaagtgct taccgatatt acatcaaagg ttagggttgt aaagatataa
    12841 tcattttttc tacaggcaca aatattaacc aatcagcatt cattaatgga gaaaaacgac
    12901 taataacatg taacaaaact aactcagata tgatatagat aagttcaatt atgatataag
    12961 atttaatagt actccctacc atactttaag ttagaatgga ttgattgaat gaaaacaact
    13021 atttccagcc taatcactct tgagatcgga cacgttgaaa aattagctga tgaatgtgta
    13081 gctgacattc tcaccgaatt accaaatgag cagattcagg ttggtatgca tgacgcaaca
    13141 gcctttatat tcctacttaa taacaaacgc ttcacgctta tcaataccgg ctctggctct
    13201 ttagccgtca gaacctgtta acccctctct gctacctgcg cgaatggctt agttccctgt
    13261 tcgcgcagtg ctacattaaa cacactagta aataatttgt tttcataaca aaggattagc
    13321 catgtctaaa aaacgttcca tcaaagaggt tcaggacttc cgtgacagtg taaaacgagt
    13381 agtcgctctc ctttcaggta aaaacatccc tgttgcagaa cgaggagacg acgcttatgt
    13441 acgctataac gatgatggag agccaattct cgtaaacatc ccatcaattc cggataacgc
    13501 aacaccggca ttgatgaatg ctgtgcgcgg atttctcgat catgaggttg ctcacatttt
    13561 gtttaccgat attcgtgtgt ccaacaaaat gagagaaaaa ggacgcgttc cttcccggtc
    13621 actatggaat gcattagaag acgtgttcat cgagcgaaaa atggggcagg tctttaacgg
    13681 aacgagacgt aatctgatgg caactcagcg ccttataatc gaaaaagtct ttaaaccaaa
    13741 ggcttcagag gctattgctt attgtggcaa agatcagcgc gcgctttttc taaacttctt
    13801 tctctgtccg gttgtaagag cctgggatgg ccaagcaccg ttcgtagatt tcatggatga
    13861 atactggcct gtcattgaga aaccaatttc attattaaaa gaacatggta tcgatgtggc
    13921 cgtgcgtaac atgtcctgca ccgaggattg tgtaaaggtg gctgcgacca tagctaagat
    13981 cctcaaagac actgaaagtg aaagcaaagg taaggagtca gctccgggaa aaacacccga
    14041 tccttcagac gctgaccaga cggatgcctc tggagaaaac aatgaagaca acggagatca
    14101 tgagacaccc gaagcgttag atagccacaa atctatcaaa tctgaatcac acagtaagca
    14161 caaacatgat attaacgaca gtgatgattc agataattct gaatcatcag aaacaatatt
    14221 cgatgataca gaaaatgata aagaggtatc agattctgat gcttctgata acgcggcgtc
    14281 agaatcatta accgctgacc acgaaaaaag aaaaacgaca gaagacggct cttcagatat
    14341 tccaactccg tcaaaaataa gtctggaaga ggctttagag gagctggata gcatagaaga
    14401 tgaagtcgga ggcatgacag aagatgctct atccgaaacg attaaaagcg agttaacaga
    14461 aagctcgaaa agcgaataca ggccatacaa tcgttcatac gacttcatcg gctcgattga
    14521 tcaggcagaa gcccatatca aacggcttat taaaacattc tccgatattg atttaggagg
    14581 atatccaatc agccgctatc gcatcgttcc tgaaggcaac cagctcttcg acaaatatat
    14641 tgaaaagcat ctttcgtcag gtgtttcgtc gacgctggca aaagacctgg agcgagcaat
    14701 agcaagcaga aacagagttc agtttatacc gggccagcgt cgggggcgca ttcatggttc
    14761 tagtatctac agattagcaa tgaatgatga tcgcgtgttt cgtaaaaaag aagaatctaa
    14821 agccgttaac gcctgtgttc aacaagttat tgatttatca ggttcaatga gtggtataac
    14881 gatacaattg gctcttgcaa gtgcatatac catcgccgat gctcttgatc gaataaatgt
    14941 tcccaacatt atcaccggct tcactacatt tggtagtcat atggcggcag gagaacttaa
    15001 ggctgtcaag tatgagttct ctcgctttga atctttaatg ctacctatca tcaaaaattg
    15061 gaatgaaaag gtaaattctc gcgaagttcg ctcacgtatg gggtgcgtag gctacacatt
    15121 cccacttctt aataacgtgg atggtgaaag catagccagc cttgcatcgt tattttccgg
    15181 tcgcttggag gacaggaaga tcatgcttgt tctgagcgat ggcgcgccgt gggctgttgg
    15241 gagagatttt gacgctcatt tgcgttcggt tgcgaagcaa attgaaacgc agactgacat
    15301 tgatttgatg gcaattggca tcatgactga cgcaccggag agattttact caaatcatgc
    15361 cctggtaacg agcgttgata gtcttggttc atctgtagtt actgaactat ctcgtatcat
    15421 tttgaagtga ataaaacagc cttaatgata agtaaccact tacgatagtt aatggtatat
    15481 ttatataaga agttgaacgc tcattagaga acaaaggaaa aacgcatgac gactactgca
    15541 ctgcaaaatg aaaaaaatcc ttctgattac cttgtttgca agtggtgcgg caaatcattt
    15601 cactatttta agtcccatgt agccaatggt aattgcgagg gcattcctga gtcagtaaaa
    15661 gatgccgatc ctgacaccgt actgaaaatg tacacaacgc agtttccaga tgaaccaacg
    15721 ctatcgaaaa aggcacttga tgcaattcaa gctaaacgtg ccgagcaaaa aagcgaaatg
    15781 gccaaatctt ctggcttgac cagtagccct ggctacacag gcacagttga gtacaagaca
    15841 gatctggtcg cagctcacga actgctaaac gtaacggtgg aagaactcgg aacaaaacgt
    15901 gggacgccgc tcatggttag cgtcaacgtc aatacgccgt ttccagagtt cgttccagaa
    15961 gtgaagaagg gatacgtata tggcgacttc gaactgatca aagacatttt catgatgctt
    16021 gaacttggca taccaggcta tttgtggggt catgcaggaa caggcaaatc ttcattgcct
    16081 acacaactat gtgctttgct caatcgtccg ttaatccgtg cccaacatac agcatcaatg
    16141 gaagaggctc atgttacggg acaaattctg gcgcgtgatg gctctacgta tttcgagcct
    16201 ggcttgcttg cgctcgcaat gaagcatggc tgggtttacc tcgcggatga atacgacttt
    16261 gcgtttccac agattcttgg cgtgtatcag ccagtgctgg aaggtgaagc gttggtcatc
    16321 aaagaggcga ctccagaatg gcgtcgcatt actccgcatg aacggtttgc tttcattggc
    16381 actggcaaca cgaacggatc tggtgatgaa accggcttgt accagggtac aaacatccag
    16441 aacgccgcta acttttcgcg ttttggcatc gtttcgaatg tgaaatacat gagcaaagag
    16501 gcagagatca gcatgttgat aaatgctggc atcgtggatg aatacgctga aaagatggtt
    16561 aagtttgccg gtatcgttcg cgatggatac gaagaacacc tgatcagtca gccgattggc
    16621 cctcgtgaac ttttgttgtc ggccaagatt ggaatgatgc gaggcgactt tgtgacaggt
    16681 attgagcgtt ctttcattaa caaactccct tcagcttctg cgcaagcggc acgtgaagtt
    16741 gttcaaaaaa tatttggtta atcgtgcgta aaggatgttt cggctctctt atcgctgctt
    16801 ctgaaactgg caaggcttgc ctggtgtgtc cagacaagcc cgattgtcac caatcagcaa
    16861 aagaagttgc gatttcgatg tatgggaagt tcgtaggctt ccccaatgac aaaatcaaaa
    16921 aaaccagaaa ggtaaaaaca catgaaggct ctgatggttc gaactgactt ctctcttggg
    16981 gagtcggctc taaaagcaga aaacgcggtg aagattgcca gagaagctgg ctacaccgct
    17041 gtaatttcgg cagatagcat gaatattgcg agtgttattc cactacaacg tgccgctggt
    17101 gacgacatgg cggttatttg tggtgtgaaa ctaaacattg ttgatgatcc cacatacgag
    17161 taccgggcta aaattgctaa agagtctaag ggatgtatgg aatcattaga gcgaggacgt
    17221 aactactcgt ttaccgctct aattaaaaat gagcaaggat atcgcgacat ctgcgaacta
    17281 atgacggtgg ccaacacacg agaacagttc tactttgtac cgcgtctctc gctcgaacag
    17341 ttggtttcta catatgccaa aggcaacatc atcctgctca cttccgacat cggtagcgtg
    17401 ttccaacgca acgattttgc aaaaatcata agcacactga ttacagcggg tggaaaagac
    17461 aacttctata gtgtggttta tccgcaccct accccattct acgaccagat taacgtccgg
    17521 gcgatgaaag tcgccagcgc attgaaaata gagccagtgg cgttctatcc cgcttattac
    17581 gaatcgatcg acgatgcaga cattaaagac attgcgcaca tggttacgaa caacatcaaa
    17641 atcgaccagc cgcatcgtct gcgtatcccc caccagcgag ataacgccgt caatggtcgc
    17701 cgccatctcc ttgaggcgct gaaaaccttc tccgttcgca tggatgtgcc ggtaacagct
    17761 gcaatggcct caacaacgca ggataccatt atcgatgcct gcacatggcg ctggcatgaa
    17821 ttgccaccag cactgccaaa gatggcagac gacgagcctg caacgcttat gaaactggct
    17881 gttgcagggc tgcgtaaacg tcttaccaca aaagagtttg gctacacacc acctgcttct
    17941 gagaacaggg tttatgttga gcggctaaag tacgaaatgg acacgctgac tcgcctggga
    18001 ttttgtggtt acttcctgat ggtacgcgat ctgatgaatc acagccgtga aactggcatt
    18061 cctgtcgggc ctggtcgtgg ttcctctgcc ggttctctgg tggcgtggtg cataggcata
    18121 accaacgtcg acccaatccg tcacggtctt ctgtttgagc gtttcatcaa ccctgagcgt
    18181 ctcgacttgc cggatgcgga cttggacttc agccaggcac gtcgccatga ggtgatcgag
    18241 tatctgaatg aacgctacgg cgaagattac gttgcaggca ttccgaactt cacctacctg
    18301 ggcgcagcct cagcactacg tgataccgct cgtatttatg gtgtggagtc cgcagatatg
    18361 gcggtatcaa aagagctgaa gaacgtcgag gatgatagcc ttccattgga ggagctgcgc
    18421 gaacaactgg caagtctcga caaatacgcg acaaaatacc ctgatgcatt caatgctgcc
    18481 tgcaagttac aaagtcttat gcgtggcttt ggtagacatg cggcagggat gatcgtagca
    18541 ggtgttcctc tgacagaacg tacaccggtt gaacgccgtg gtgacgcgcg ttgtatcgca
    18601 tttgacaagc gttactgcga ggctatgggc ctaattaagc tggacgtact tggtctggca
    18661 actctcgatt tgctcgatag tgcaaaacgc tacataaaag agaacacagg tgaagatatc
    18721 aatcttgatg ccatttctct tgaagatcgc aaggtgctgg atggttttgc tgctgggtac
    18781 actcaaggtg ttttccagct tgaatcaggc ccaatgcgca agctgcttaa agatttaggt
    18841 ggtggaattg agccaatgag ctttaaaacg gttgttgcta cgactgcgct cttccggccg
    18901 gggccaattc agtcaggcat gttggatgac tatgtttctg tagccaaagg ctttatgacg
    18961 ccggaatcat tacaccccgt tcttgatgaa cttaccgcag aaacaaatgg cgtgattctc
    19021 tatcaggaac agacgatgaa cgcgactcga ttgcttgctg gcttcacaat ggctgaagct
    19081 gacggcgttc gtaaagcgat cggcaaaaaa gacatggaaa aaatgaagag catgggcgag
    19141 aagttcatcg ttcaggctca agctggctgg atagacgttg agctggaaga tggcactaca
    19201 cagcgcattc accgtgcgga acattttaaa tgcgaagacg gaactctgaa aactgtcgaa
    19261 gaggcacttg agtacggcgc aaaactacct ataaacgcag tacgcgttac agcgtcacat
    19321 ccagggctat cagagatgaa agcgaaggag atctggaccg cattcgaaaa gaatggtgcc
    19381 taccagttca acaaatcaca ctctgttgct tactccttaa tcagttatca atctatgtgg
    19441 ttgaaaactc attatcccgc ggagttcttc gctgctgctc tcactattct tggcgaagat
    19501 aaacaccagg ggctggttaa agatgcgctg acctatggta ttcgcgtatt gccaccagac
    19561 gttaacgtgt catctaaccg aatcgagatc cgcacgcttg aagatggcag ccaggtgctg
    19621 tatgcgccct tctctgctgt gaaagggtgt tctgagaatg gctgccaagc catcatgaga
    19681 gcgcgagaaa aagttggcgg caaattcgag tcactggcgc aattcgaaga agctgtcgag
    19741 aaacgtgcct gtaatagtcg agtgcgcgaa tcactgcaaa aagtaggtgc gtttgcatcc
    19801 atcgagccag gtagtctgcc agcaactgat ccagagcgtc tccgcgacca ggctgaattg
    19861 atgggaaacc ttgtcataga cgcagttaaa gcctcacgtc cgtttgagat gaaccccaaa
    19921 cgttcggctg agattaacgt gctcatgaca cggatggcgg ctgaaatggg attaggtgat
    19981 gaactaatcc gccctagcat tggcattaag ccgaaaatca tgatcattct ggacaatgcg
    20041 aacggcaatg atgctcgaac cggttacttt atggagaacg gatacgacga ctttaaggcc
    20101 aagctattga cggctggaga tttacgcatg ggcgatctct atgtcacagg cgtttgcaag
    20161 aaggttaaag acaaagaaaa agactacacc aaagacgaga tcggccagtt caccgacttt
    20221 atacgtgaag agatcaatct ggtgcgtccg acctatgtgc tgacgtgtgg cagccgggca
    20281 acggcattgt ttaacaacaa aaacaagcca tccgatttgg tcggcaggaa ggagtacctt
    20341 ccagaccttg acgttactgt cttttacggg tttaacccca acattcttta cttcagacct
    20401 gaagagggag aacgactaga agcgatatta gccgacgtag cagaaaccat taagacgtga
    20461 tcccataaac atggcctcta tggccatgtt ttcccttatc ccctcatatc ccctatcctc
    20521 gaactccttg ccttatcatc acaatgatat aatcaatata aaatgataag taaaaaggaa
    20581 agcacatgat caccgatatt tacgagaaaa taatgtctga tctcgagttt gaccgagaca
    20641 atctggagga agtctggcgt agacaacccc gccttttaat ggagtatggc tcaaaactcg
    20701 ctcatgcaga aagaagtgtc gcagaggcaa aacttaacct tgaagctgtt gaagcaaagc
    20761 tatacgacac agagcgtaag aacttgagta tgaacggcat taagttcaac gagtctgtac
    20821 tggacgctaa ggttaaaaca aacccacagt atctgtctaa acggcagaag ttggatgaag
    20881 cacggcacat cgcagacata tacaaacatg ctgtcgccgc cttttcgcat cgccgagaca
    20941 tgatcgttca ggcgtcaaag atggccatcg ttgaattaga gcgattaggc tctgaacgct
    21001 ttattactcc ccgttgattt ttgatagata ataagtaagt actgatctat catttaacag
    21061 ctcgaaagag ccacgaatga acgaaagccc aacgcgcata gcgccatcgg ccaaatcaca
    21121 acaaggagaa acacatgtct aagacattac ttgatttgct taacaaaact cgtgaagaca
    21181 ttgccgccaa acgtggtaac aacgttgatc tgactcgctt aaaagacggc gtcaactata
    21241 tccgcatctt cccgaataaa gacgacccaa acggtaagtt cttccagact ttcggtatgc
    21301 actacgttaa gtatcagaac gaggaaggta aagaagcaac caacgcttat atttgtgagc
    21361 aacatactca cggtcgcgct tgtcagctat gcgaaatggt gatggaaggt cgcgctcgtc
    21421 acaagggtaa caaagcaatg gaagaacgca tcggtcaaat gcgtgccact cctcgctatc
    21481 tggtcaacgg cattctttct gctcgtgagg atttcgcaga tgctgagaaa tgccagttaa
    21541 tcgagctgcc gtctactgta ttcgatgata tctgcaaagc aatcaccgaa gacatcgctg
    21601 atgatatcgg caatccactg agcaaagagg aaggctacgc attcctgatt aaacgtactg
    21661 gctctggtcg cgataccaaa tatgacgtct cgcctaagcg taaagtctac aaaggcgata
    21721 tcgaagataa attctggaat acccagcatg atctgatcgc atacgcaaat caggctgatg
    21781 aaactcgtct tctctcgaca gttcgcacta tgggtcgtct gattggcatc gcagcaccaa
    21841 ctgcctctgc atcggcacca gcaatttcct ctaccgcgaa aacatcggcc gcggcactgc
    21901 ctggatttgg ctctgtcact ggtcatacgg agggtgcgac tgctgtagct accgctcaca
    21961 ccccagcttc tgaaccaacc agtctggttg atgaagaaat cctccgtgcc gttgaaactg
    22021 aatttaaacc agaagcaagt tccgctgccg ttgcagtatc agtcaaagag tctgaagcag
    22081 tcgcagcgac atctgtagca gccgcatctg cgacggaaga tgaaggtctg gatgacctac
    22141 tgagagagct ggactctctg taatcccatt acgtgaccag taaggcgtct acggacgcct
    22201 tactttttgg aaggaatgta ccggtgaatt atctcttcgt agatggcaat agcctgggtt
    22261 attaccacca acaatctgac aaattgcaca acggcgaaat ggaagtacag gctgctttcg
    22321 gctttgttaa gaacgtccgt cgttatgcct ccatcctcca tgcccgacct atgattcttt
    22381 gggatggatt tagtgacaag cgtcgcgact tttacccgga ctacaaagca aatcgcgacg
    22441 acgatcctga tatgaaaaag atgaaggaag gctttgctat ccagaagcca tacatcctca
    22501 aaatgatgac cgcgcttgga gttacccaac tcattgcaaa agatgcagaa gcggatgatc
    22561 tggccgggct gctggtagcc agcattgcac cgcagccaat tgttgaacac atctatctgt
    22621 taacaggcga tagcgactgg cttcagttag ttcgcgaaaa cgtaagctgg gtaagcctgc
    22681 gcgaagacgc caaaaacaag caggttaatt ttgagcaatt tgcggagctg acaggattcg
    22741 ctactcctcg cgcatttttg gaagcaaaag cattacaagg cgataaatcg gacaacatta
    22801 gcggtgttgg tggcattggt gctggcggtg cgaaagagct gctgcatgaa tggggaagtg
    22861 tcgcaacgat ggtacgcggc atcaacgacg gctcaatcgt ggttgacaaa ggacgccata
    22921 agaccgcctt caacaaacta gcgaagaatg ccttcaacga gaaaacaggc tgtcgaatgc
    22981 tcgaagcgtt caagagaaac atcacgctaa tgaacctgat tgagacgaag tttccgccta
    23041 ccgaaatcga aacaatcaaa ggcaatcgtg acgtgaaagc attcgagcaa ctgtgctacg
    23101 agctgaattt ccgttcgttc cttgaagacc ttgaagtgtt tgttcttcca ttcgaaaggt
    23161 attgctaatg cttaaatcga ttattaatgg cgctacaacc acccctaccc aactggcaaa
    23221 agagattgtc ttttatcacg gtgagtacgc tgtcatcgca ctgccgtcaa ttctaggcgc
    23281 tgccggaatg aaagcgacag atcgcgagtt tggattagtc agcgagcagg tcgtaaaaat
    23341 cctcgctcgt gtatccagac tccttaacca cgatgcgatt gtattcgacg aatccgccgc
    23401 tttaaaacga atcaacgaaa caaaaggagc ctgatcatgg caaaaggaaa atccgcactg
    23461 gcacttgctc tgaaaaagaa aatcggtagc aacgacgaaa ttcagaaagt aactcattgg
    23521 attgacacag gctttcctcc gttaaacaaa gcgatttccg gtcgttacga tggcggtttc
    23581 ccatgtggtc gtatcgtaga agtattcggg ccaccaagcg cggggaaaac ctttttggca
    23641 acggcagcaa tgatctccgc acagaaacaa gatggtctgg cggtattcct cgatcatgag
    23701 aacagttttg acgttggcct ggctgtagcc aatggtttga atgcagatga ggatgacggt
    23761 cagtgggtat acaagcagcc tgataccttc gaagactctg tagagttgat cggcacaatt
    23821 cttaaattgg tacgtgatga agagcttatt cctgaatcag cacctatctg tatcgtggct
    23881 gactcacttg cgtctatggt tccgaactcc aaagccgaga agttcgaaaa gatggctgaa
    23941 ggcactgcca aagacaaaga tcagctaaac atgaacgaca atacggcact ggctcgtgcg
    24001 acaagtgcga acttccctac tctggctttg tgggcacgca aatacaacgc ctgcatcatc
    24061 ttcttgaatc aggttcgcac aaaaatcggt gtaatgtttg gcgaccctac tacgtctcca
    24121 ggtggagatt caccgaagtt ctacgcttct gtacgtatcc gtcttggtgc atcggtgatg
    24181 aaggatggta aagagaagat cggccaggac gtaggcgcag aatgcatcaa aaacaaagtt
    24241 gcaccaccgt atggcaaatg cacctggaaa ttctacttcg atcctactcg tggcctcgac
    24301 gttatcgaat cgctcgtcga gtacatgctg gaagaaggat acctgccaaa gaacgccagc
    24361 gggcgagttg aaattggtga caagaaatac accaaatcgc agatcgtcga gatgtatcgg
    24421 gagaagccac tggctgaaat cattgcggct ttgcaggcaa tcgacgaccg aagagcaaaa
    24481 gacaacccca ccgagtcagt agaagagtaa acacaaggcg tccacaggac gcctttttta
    24541 tctcttgaaa atatataagt acttacttat tattttcgca taacaaccac ataggaaaac
    24601 acatgatcaa aatctatcta ttggcagtag ccacaggcgt ttcagtggct ctcatctacg
    24661 gtttactggt tccgtcgctg atttctacca agagtgattt agccgtcatg tttggagtta
    24721 tcgttggttt tggtgctcct gtaatcggtc ttattgctgg tcgtaagttt atcaactcat
    24781 taatcaaagc aaaggggaaa taagtaatga agaaaggttt acttgcagtt gctctggcgg
    24841 ctatttgcac aatgggtctt actggctgtg atcgcgtgga gcctggatac gttggcatca
    24901 aagtaaacaa attaggtgaa gacaaaggga tcggtgaagt ggttggcgtt ggtcgccaat
    24961 ggacaggtct taacaccgaa ctttacgtat tcccgacctt caaacaaatg aagacctacg
    25021 acgaaccgtt cacattccag atgagtgacg gtactgctat tggtcacaaa attggcgttg
    25081 cgtatctggt taatcgtgac aaggtaacga cggtgttcca gacctatcgc aaaggcgtag
    25141 acgatatcac cgaatcagat ctgcgtcaga aaattgccga ctctctaaac cgtttggcca
    25201 gccgtatgac cactgactca tttatcgacg gtggtaaggc gcaattgctg gacaacgcac
    25261 tgaaagatat tcagaaagag atgtctccgg ttggtattga ggtactgagc ctgtcatggg
    25321 ttggaaagcc tgattaccca aaaaccgtca ttgaatctat caacgccaaa gtaacggcta
    25381 accagcgtac tctgcaacgt cagcaggaag ttgaacaacg taaagctgag gcgaatatgc
    25441 tacgtgaaca ggctaatggt gaagctgatg ctatccgtgc tcgtgcgcaa gcagaagcag
    25501 acgccattcg tctgcgcggt gaagctctgc gtcaaaaccc gaacgttatg gaactggaag
    25561 ccatcaataa atggaatggc cagttaccgc agtacatgac tcaaggggct aacactcctt
    25621 tcattacagt gaaataactc tcattaagaa gatcaggcgt ccagttggac gccgttttat
    25681 tctcgttatt atcacacaca agaaaacaat ttgttaaaag gttaagttaa ttatgcccaa
    25741 ccacattaca aacgaaatcc gagtcatagg tggtacaaat aaacaacgct tagatttcat
    25801 acgtgccata actaacaaaa aaggtctaat tgactttaat accatttgca gacgtccgaa
    25861 aagtatagaa atggaagaaa acaatcatat caggcgcatg gcctctgcaa tggcgggcga
    25921 aacagtttac gaccacatgt tcggtgaatt aaagactcca gaacaggtcg aattagctat
    25981 gcgtgaatca ggaatgacac gcaaggaaat tcgcaaaatc aaagagcagg ctcttatgcg
    26041 gcttgagaat tatcgtcgct atggctatta ctcatggttt gactggtcgc gagctaattg
    26101 gggaacaaaa tggaatgcct acaacatcga aatgcccgta aaacgtatcc ctacacgtat
    26161 taagtacggc cataatttcc gccatacaca cgttcgagca tatgcaaaac gaatctataa
    26221 aaaacgtctt gcaagacatg ccgcaactgg tggtgagttg gtgattcgct ttgaaacagc
    26281 ctggtctatg ccaaaaccaa tttatcaagc gatggctaga aagttcccgc accttgagtt
    26341 tgtaatacgt tatgcagacg aagacttcgg caataactgc ggattcattt tacttcgcaa
    26401 cggcaaatgg gaatatgact acattgcacc ggcgaataac aaacaatcgt cagaagaaaa
    26461 aatcaaatgg cgcaagttcg cctttgagtt atgttgtcct ggtatcactc cacaagaata
    26521 cggtctaaat gaagaatacg aatacgccgg atattaaaaa aaattgtcat ttaactttac
    26581 gcatgaagca tagaggttaa taagatgaaa agaaaaattg aagaaccaaa aacgaccctt
    26641 aataaacatg gggaaaaaat tcaaacacat ccagcgttcg gtcttgtgaa aacaagccgt
    26701 gttagcacaa ctggcattcg cctatttgat tcagaactgg aacatagaga ctatattgaa
    26761 ataggcgttt acgaggcgga actgtcctta gacaatgaac gaacccgccc acaaagaagt
    26821 tgcaatagac ggcctttagt tgaaattaga cttagccagg cacaatgggc tgctatggtt
    26881 agcagttttg gagtgggcga aggtgtgcca tgcactattt catataaaac cattggagac
    26941 tcaattagag ttcatgatat tgccgagcaa aaaagtattc ggcaaaaatt caacgatcaa
    27001 ataaaggccg ctacgaatag tgagattgat cgacttaatc agcaagtttc agagctaggt
    27061 gaacttgtga aaaaaggaag agctggaaaa cgtgaccttg aagaactata tagaaaacta
    27121 gcatctgctg tagctaacct tcctgaaaat ctgtgctttg ctaccgaatt aatgcaggaa
    27181 caaatggatg aaattgtctc atctggaaag gcggagattg aggcgtatgt tgcgggcacg
    27241 gttatgcgta aagggttagc agcattaggc acagaatcat gcattaacga tgatcctatt
    27301 gtctctatca ccaaaatgga aagcggtaaa gaattactgc cataaatgca tctgaggtac
    27361 tgacaatgct tttaattcaa cctgggttcg gtttaaagat aaaaaaaggg catatgtttg
    27421 gtgagcgaga ttccaaacga aaaattctgt ctattcgatt accatttatc accattcatt
    27481 ggttaaacag agagtcaacc gattattggt ataaatgcgc aagagcagca tttaacgacc
    27541 ctgactggtt tgtagaaaat caccacgcgg ttcgtcaggc aaaaagaaag gctaatacga
    27601 catatatgaa ggcatatcag gatgcctggg aagaacatcg tggccgatac caaaaaaaca
    27661 ttgaaaaact tgaatcagaa aatatggacc taagaagaaa actcggtgaa gcacgtcgag
    27721 acatcgaagc atataaacgt cttgtatcgg agtgaatata tctttgcttt attttttcaa
    27781 aggtgattat tttcctattt ataaggagaa gtaaacatgg cagcaattaa gaaactctac
    27841 gatgccgcaa acgtggctct ggatgttatt gatgatgaag tagcaaaagg ctttcctgaa
    27901 cctgattggg cgcatcagct acgaaacgct atcgcagaaa tgaccccacc agatccaacc
    27961 cccgacgaga cagactggca gcgattcatc cgtatgtacg ctcaggaaat aggtccaacg
    28021 ccaacggcag agcaggcaat gctgctgaaa tacttcaaag aggcgggaga ggatttacca
    28081 attgatgact cagcatattg gttccactgc gcatggcgta agtatgacgt gatattcaca
    28141 caaggcatgg gaagcaaaga tatggttgtg tggcatctac tccatataga cacagccgtt
    28201 gacagagtta ttgaacagtt tttccctaac caagaagatt gatcgcctat tcataactaa
    28261 caaaataagt aaacactaac cacaaaagga aaaacacatg agagttttag ttcgaatcgt
    28321 taccagcact gtctatgacg tgtttccgct ttttatggtc aaagtcgatg gccttaacga
    28381 tgaagaaact gacgcgctga tccagcgtac tcttgttgaa tatacaggtc atgacgctga
    28441 ttcagtgatg gttgatgatg atggtgtttg ttggcataac ggcaactgtt ggtacgtaga
    28501 agagactcaa caaatcagta atgaagatgc tgaacatctt gagcgtattt taagcatcag
    28561 cacttttgag tgagtttaca gtaaagttta tataagttag tatctaccta tcatgaagat
    28621 ttttattgaa tacttgttac tcatcgtttc aatagctttt gtcatcgact gcattttcac
    28681 tggtgtcatt cgtaaagtct tttccccggt gcacgacgta gtcataaacg ctttggctat
    28741 cgtgctcgta tttaattcag catttgatgt aatcaaagag gtggcagcat gaagaccatc
    28801 ccattcgcgc tgttgtttct ttcttcgatc gttgtggccg acaccactgt ttatcagtgt
    28861 gaaatgtctg tagccgacgt taagaatggc gctcttaccg acgtcataaa agcaccatat
    28921 ggagcgatgg tcgtagacag cggcgaccag ttctatgttg tgcgtgacga tcgagtgttg
    28981 tcatccccat atctcacaaa tcgtaatggc aaattaaccg gcgtcggaga agaccacttc
    29041 gtatacaaca aatacaaggg cttctatggc gttcacgctt ctcagcaaag ctaccttttc
    29101 gatgactgca aggaggttgg gtaatggcat taacactggc aggtctggaa atcgagaaaa
    29161 caagcggcta ctggcgtgct aagggtttca agcagcctgg cattcttgag cgtctggaac
    29221 gtgaagatgg gtatatcgtc caccagcggc gtgaatggcg tatgtacgat ccagaaacag
    29281 gaaaactgac tacaaaagcc ggaacacttt ggggtctgtt aaagaaaata cactaaatgc
    29341 aaactgactg cggcacgttc cgcagtcata tttcatagtc gtcaccgctg acagcataca
    29401 caatcaacta ccgctgatag catatcgaga gtctatctca ccgctcacag catactttac
    29461 tcgatttttt accgctgaca gcatacttaa gacattgcat gaataatgtg taccggtatg
    29521 ggtataacca gaacaaaatt accgctggca gcatacgaat gtctgacata tgccattaat
    29581 taccgctgat agcatatcca aacaaaaatt cctcaataaa acaccgctga cagcatacgt
    29641 tctatcaggg agtagcaggc aataaatgcc tttcactaca agcaatcagc gcaatagcaa
    29701 tagaatgtta gtgagcgcaa acctatatgg aatgcactct tcgaggttag taaccactgg
    29761 ggaggtatga cagagcattg agtggtgata gatgatttac cgctcacagc atacgttcat
    29821 ctcactatac cgctggtagc atatctttaa ccgttcacag catacttttc agaaaaatag
    29881 ccgctgatag catacatttc accgctgaca gcatatcaaa gcagtttgag actattggaa
    29941 aggatctcaa tcatcttgat attttcaggc gtcaaattct gcgaaagttc ggttatcttg
    30001 ttgataatgt tctgtttggc atcaatttcc ccagctttct catctggttt tttgggttcg
    30061 atgtcttcag gttttggcgg tgcgactttg agttttggat tgcggctgtg aatctggata
    30121 tagatcgacc gcccacgctt aatctcgctg tattcgagat agcccaaatc ttggagagct
    30181 tttaagccgt tacgtatagt ctgattctgc gagctgacat tcctgctact caaattgagt
    30241 cgcgcacgca atcgagcaag cgataccggc gcaggcttgg ttggaagact ttcgatgaag
    30301 gtgtacagag cctgtgctgt ttctttgcgt ggtagcttat tgataacctt taactgcaaa
    30361 agaaccttat ggtcaaagcg atatagttcg gccagcttcg gttctgcata gaacaccacc
    30421 gtatctttct gctcgttgta gtccacgcta ttgatgaggt gcaccatcag aagcgagatc
    30481 ttgttagagc cgtcgacgtt cttttcttca tacgttctct ggaaagacag agttgtacgc
    30541 atgatcttca aaagactgtt tgtaagccgg tcgcggagtg ttttgcggat ctgtgacgat
    30601 ggatagccac aaaacttagc aaatttcgtg atgcttaact cgacacgacc gttaggttcg
    30661 ccgtattctg ccagcgaacg cacaacgccc acccacgttt tgaaatcatg atccatgtcg
    30721 agacgaggac cggttatctt gatatcggaa tagccttcag aacgggctac ttcgagctgg
    30781 acaagctcct ttgaagcatc gatctcattt ggcttgttac gtttactgta ttttgttccc
    30841 ttaagcgtgg ggacgaacaa ccccagtcgc atcaacgcaa ttggttggac tgtattgttg
    30901 ctattaggga caagttcccc tgtgtacaat tcaagggaac cttcttcaaa gttgtcgatg
    30961 ttatcttcta cttctttgtt atttttacct tttttatttt ttgtggacat gtggacacct
    31021 ttgtcattca accgctgaca gcatacttga tttgccgctg acagaatacc aaaaacagtt
    31081 ggcagcatac ggtgaaccgc tgacagacta tcaattaccg ctgacagcat acatgaacat
    31141 gacttcagac cagtcgtggc gcggcttaca gcgatcgggg atcttatttg atctatataa
    31201 ggatctatct atggatctct ttattaggat ctatcctgtg gatatgtgaa taattaaaac
    31261 aggcatttac taccttcggc gcacctggtg ggttatcgtt gcctcggcta acaatcacag
    31321 aaaaatgaca tatggatcta aaacgcacgc gctgggttcg tcgtcttgaa gacggctcct
    31381 acactatcga atcaaattcc aacctgaata agcagaagtt gctttgtgac atctgcggta
    31441 tagcggcgaa gtgcccgatc tacgaaacca gaattaaact tgataaggct ggtgtgaatt
    31501 ttcatttaaa cagttgcatc aggtacgttc cattactcgc atttcgtaaa ccgatcatcg
    31561 gattggatgc cccctacttc aacacactcc gttcaggtgt gacgtggcga gatcgtttat
    31621 caccagacaa gctgatttgc ctcgtatccg cagacacagg aaaaatcatc cgttttggga
    31681 aagtagacaa ggtttactca ggcccagtag acgaaatgtt gcggaaacac agccggttta
    31741 atcatctctg tatgggtggt gagaaaatcg agaaggtaga agaagtgatc cgcaaatcct
    31801 acggacactt tctgaccaaa gatagcctgc tcaccgcaat ctacatcaga catgtaaaac
    31861 gtgagttcga cctcgaatac cacagcgaag aagagcttaa ccttgttgac ccacgtccaa
    31921 aagctggcgt cataagcata aacgcagcgc gtaaaaagcc cactgacgcg ctgtaaccct
    31981 ccagatcgta tattggcgta gatagaatct acgcctcctc aaaatagctc tcatagcgtt
    32041 ctacagtgat cctgtcttat ttttagtcat acagacaagc aaagttgcgc cacaataaat
    32101 aggtatatac ttacttataa attttgtata ttaagacgct cgtttcattc ctaacatacc
    32161 gttatgcata gttgtttacc ttctcattgc tcttaaaatt tgtatcaaaa taaccacaaa
    32221 ggaaaaacac atgactttgc catacggcgt catttctgac tgccactacc acaaatggga
    32281 tgcgttctcc acgacgaacg cagaggggct taactccaga cttgaaatac agttggaagc
    32341 aacgaaagaa gcagccatcg ccatgaagaa ggccggttgt aagtacatgt tggttgccgg
    32401 tgatacattt cacgtccgag gaactgtgtc cccttctgtt ttgcattacg taactgaaac
    32461 gtacaagtgg attatcaacg agcttgatct gacagtagta atgctggccg gtaatcacga
    32521 tcttgaaacc aacgattcag tatatagcgc caacgcagca gcatcgctga gttctatcgg
    32581 cgtggtaatc gtatgtggca aacgcccaca ctcaataaaa attggtgatg tgactgtcca
    32641 cctgattagc tggcgtaaca atcatgcgga gcttatcagc gatctgaaag cattacgtaa
    32701 gagcgtagaa ggtgataatc atgacgttgt tatccataca tccattaaca aagccattcc
    32761 aacaatgcct gacgtcggta tcgatgcgca ggagttaaag gatatcggct ttcgtctcgt
    32821 gcttagtggg cattaccaca accacaaaga ggtcattcct ggagttatca gtgtcggtgc
    32881 actgacccat caaaattggg gagatgttgg atctctggct ggttacatga tcgtaaaccc
    32941 ggacggcagt ttcagtcact acgaaaccag tgcgcctaaa ttcattaacc tggaagatga
    33001 tgttgctgat gaccaaattc gcggcaacta cgtgcgtttc cgcgccgtaa ttgagaacga
    33061 tgaagaaggc attaagtacc agaacatcct caaaacaatg ggcgcaaaag gtgtcgtgtg
    33121 caacttcatc cgtaagtcat caatgatgga agggacagcc agcacaactg aaaccagcaa
    33181 aatcgatagc ctgggagagt cggtatctgc ttattgcaag attgtccacg atactgacgg
    33241 cggatttgat ctgagcaagt tggatatttt gtgtcaggaa atcctcaccg aagcggagag
    33301 ttcggaggct gtgtgagaca aagtcgttat gggagctttc gagactttgc catcacgatg
    33361 aaaagacttg aacgaggcca gacggtgatg tttcacaagc cctacccgcc acaaggaaat
    33421 cccgtagcgt tttatcttgg aaggttaacc agaaaaggcg tattgaggcg cagatccttc
    33481 ccggcgcata cggagttcag attgaaagaa ggtcaaaagc taacacacgg tatcaaaggt
    33541 gttatatgaa gtttttaaag ctccaggttg agaattttat ggctatcgct agcgcggagg
    33601 tcgagttaga tcagcgtggt ttagtgctca ttcagggtgt taatagtgat gatagttccg
    33661 catcaagtaa tggctctgga aagtcaacgc taatgaatag cctgatgtgg tgtctttatg
    33721 gcgaaacagc tcatggtgtg aagggtgacg atgtgttgtc taccgaccat gaaaagaact
    33781 gtcgtgttgc agtaaccatc gaggatgaag gcaagagata tgcgatcatt cgtcaccgta
    33841 aacacaaaga gttcaaaaat cggcttatcg ttcgtggtga agacggcgat atgacgaaag
    33901 gcaaagatgc gctgacgcaa gagttcgtcg agcgtctgat cggtgcatct aaagaggttt
    33961 tcatggcttc catctatgcg agccaagaag ctatgccaga tttacctgga atgtccgaca
    34021 aaaacctcaa aaccatcgta gaagaagccg ctggcgttga cagactgaca cgcgcctacg
    34081 ctattgctcg tgagcgagct aatgcagctg ccgcacgtat ggatgtggtt aaaaccaaat
    34141 tggagtcgac aatctcgacc attgaggcaa cacagtcaga aattgagtcc gcgaaagcct
    34201 cctctgaatc atgggaacaa gagcgttcta aacgttatga cgatgccctg gctgggctgg
    34261 caagtgccga agttgagtta acggaagttg aacttgagat ccgcactctt cccgaacaga
    34321 tccgtgatac cgagaaggca atcgaaagtg agcgcaaaaa gttagcctca aaagaagaac
    34381 atgacgccaa gttgctcaaa gttcgtggtg cgataactga tattcgggca agcatcaaag
    34441 ctacagaaaa tagtcaggct gatgcaatga accgcgcgcg taattttaag accaaagcag
    34501 aagaggttgg tactaaagtg ggatcaccat gccctacttg tggcaaagcc tactgcgaag
    34561 aagatctatc aacggtgaag gagaatttca ttgaacaagc acgtcaggaa attggtcagg
    34621 cgaagacact tgcagaggca atggctaaac acaaaacgaa tcttgagaaa gcgttaagca
    34681 ttgagtctgc ccttgttaaa acgacacctg atgtaacggc tatcattgcc cggattgaag
    34741 agcttacgaa acaactctca tctttgcgtc atcgtgagaa ggagattgtt gctattgagt
    34801 ctcttgtgac tcgtgctcgt actgaggtcg atcgtatatc aaaagagatt aatccgttta
    34861 ttgctcttat cgccagacac gaagataacc tggtatccag taagtctacc ttcaagtcct
    34921 taaaagatga gttgaaggct attcaggaac aaacgttgct attggaaaaa gctcgtcagg
    34981 tctactctcc tgccggggtg cgttctcata ttttgacgtc tgttacgcct ttcctgaata
    35041 cacgcactgc cgagtatctc aatacgttgt ctgacgggaa tattactgct gagtggtcga
    35101 cgatggatgt caccaaaaaa ggtgagtatc gcgacaaatt caacattagt gtgcagaaga
    35161 aaggttcaag taagtcgttc cagaccctct ctggtggtga gaagcggaag gttcgcattg
    35221 cgtgttcttt ggcattgcag gatctggtta gtaaccgggc gagtaaaaac atcgatttgt
    35281 ttatcggcga cgaaattgac gatgcactcg atacagccgg tcttgaacgc ctcatgggta
    35341 ttctggagtc caaagctcgc gagcgaggta ctgtgctgat tatctcccat aaagagatga
    35401 agtcatggtt ccgggaaact attacgctgg aagttaaaga ggggcgcagc tatgtcgttt
    35461 aaattaagcc gctcgcagtt tttgcaggta tttgcagtga tgcagtcgat aaaactgatc
    35521 aatgggcata cttccaatgg tgcggctcca cgtattctgt ggggcagcaa caatattgac
    35581 ggagtacaat ttgccgcgtt gcttggtcta atatccgaga cgccattgat gcaaagtttg
    35641 aaatcactac cacctggatg tattgcgccg atcctgatta atccttttgt tgagggggga
    35701 tatcttccca acgtcgggcc tgggtttatt gcatcccatg aaaatgaaga tcttaacatt
    35761 aatagcgaag ggttctttgg gggaatgggt gcgcatcact gtatggcttt caccaacctt
    35821 attcgacttg ccaataagcg ggtggatagt ttggcatcgc caggtgatgc ttttactggt
    35881 ttccttatcc aaaggaggga taaaaagtac agtgcggaca aactacagtt tgttggtaag
    35941 tatggagaaa tggtagaaat cgaacttcag ctcccgcatg ttttagcaaa cgatagtgca
    36001 gacagtcgga ggctgttggg catcatgcgt catttcatag caagtggcgt taaacatgcc
    36061 gtagataaac gtgtcacgca ggaaaatgag tattcagact ttgcaaactc tccccaacca
    36121 acgttgcaaa cggcaatagt aaccaattcg ttggaggcga gattattgga aaaccctata
    36181 tggggaacat ggtaaggaga ctatatgagt aaaaaaatca gcgtagttgg tgttgatccc
    36241 tcaatgagca actttgggct tgctgtgggc actttagacc ttgaaacgga cgaacttgag
    36301 attcacgacc ttactcttgt tgagactaaa gcggggagta acaaaaagac cgttcgtgtg
    36361 aacagtgacg atctgcgccg agccagtgaa atatggcgtg ttgcgaagcc aatcattgat
    36421 aaggcaaata tggttttttg tgagctaccg gttgggagcc aaaactctcg ttcgcagacg
    36481 tcttacggta tttgtatcgg tgtacttgcg tgtgtggata agccattgat ccaggttact
    36541 ccaaacgaaa tcaagcattt tgtcggcaat aaacttacta catcgaaaga agagattatc
    36601 cagtgggcta cgaaaaaaca ctctaaagca ccgtggctgc gtcgtaagca atctggacag
    36661 gatgttctcg tgaacaaaaa cgaacatttg gctgatgcgg tggctgccat ccataccggt
    36721 atgcaaacag atcagttccg ccaggtgcgc gatgttctta agtctctcat ttgatttcat
    36781 tgataggtaa gtgcttatct attaacatgg gccactatat ttagtggccc tctttatttg
    36841 gtgatacatg ataagcatcg taaaacgtaa cggccaaaca gagccgttat ccgaagaaaa
    36901 atacaaccgc gtcgtaatgt atggcgtaga aggcattcgt ggtgtaagcg catccgctgt
    36961 agcaatggga gctgcggcca gcatttttga tgggatgacc accagccagt tgcatgaggc
    37021 tttggttaaa tctgccgctg atttgatctc accagaagca ccaaattact cacaggtggc
    37081 tgcccgcctg aacattttta aaatccgcaa agatgccttc ggtcgttacg actatccgaa
    37141 cttctaccaa cacattgtca agaacgttaa caagggcgtt tatgacaagg atttgctgac
    37201 acattattcg tttgaagaga tcgaagaact cggtaattac attaagccga aacgtgacga
    37261 tctttttggc tatgcagcta cggtgcagtt gcaaagcaaa tacctcgttc aaaaccgtgt
    37321 tactggtgag attcatgagg ggccgcaaca tatctatatg ctggtaggca tgtgtctgtt
    37381 ccagaattgg gaagacgact gcgctggcaa aacacgtatg gagatggtca aaggtttcta
    37441 tgacgttaca agtacgttca aactgtctct gcccacacca atcatggccg gcgtccgtac
    37501 tccaacccgt cagttctcca gttgtgtgct gattgagtct ggcgatagtc tgaaagggat
    37561 tagtgcagct tcagccgcaa ttatcgacta cgtttcacgt cgtgctggaa ttggtattgg
    37621 ttttggccgt atccgtgcgc tgggcagcga gatccgcaat ggtgaagcca cccataccgg
    37681 agttattcca ttcctgaagc atttccagac ggctgttaaa tcttgttcgc aaggtggtgt
    37741 tcgtggtggc gcagcaacag cgttttaccc gatctggcat cttgaagttg aaagtctgct
    37801 ggtggtgaaa aataaccgtg gtattgatga aaaccgcgtt cgccatcttg attacggcgt
    37861 catgagtaac cgtctaatgt accgtcgact cgtcagaagc gagaacatca ctctgttcag
    37921 cccgcatgat gtgcctgata tgtacgaagc cttcttcaca gaccaggagc tgtttgaaaa
    37981 gctgtaccat aaatacgaag ccgatgattc aattcgcaag aagtcagtac ctgccattga
    38041 gctgttctca tctctgatgc aggaacgagc gtccacgggc cgaatttata ttgcgaacgt
    38101 cgatcatatt aatgagcatg gcgctttcat tcctgctctt gcacctgtcc gccagtcaaa
    38161 cctgtgtatg gagatcaccc tacctacccg tccactggca tttaccgacg acccgaacgg
    38221 tgagatcgcg ctatgcactt tatccgcttt taacctcgga gccatccgtt cactggagtc
    38281 tcttaaagag gtggcgttct atgccgttgc tgcactggat tcgttactgg attatcaaga
    38341 ctatccgatg gaggcagccg aagtgcctgc caaagctcgt cgtagcctgg ggatcggtgt
    38401 aaccaacttt gcttattacc tggcaaagaa tggcattcgt tattctgata ccgctggtaa
    38461 taaactggtg catgaaacgt tcgaagctat ccagtattac cttcttgatg ccagctgcca
    38521 acttgctgaa gcaaaaggtg agtgtgactg gtttgagcag accaagtacg caattggtca
    38581 gttgccgatc gaccattacc gttcttcatt agacgaaagt ggcgaaacca actttgagtt
    38641 aaagatgccg tgggaagaac tgcgtgaacg tattgcaaaa tacggtcttc gcaactccac
    38701 actgacggca caaatgccat gcgagacttc cagccagatc actaattcca caaacggcat
    38761 cgaaccgcct cgtggaccgg tgtcggtgaa atcttctaag gacggcatcg ttaagatggt
    38821 cgtgcctgag attgaaaaac tgaaggaaca gtatgaatac ctgtgggata tgccggacaa
    38881 ccgcggctat ctgacaaagg tggcgatcat ccagaagttc tttgaccagg ctatttcggc
    38941 caataccaac tatgaccctt ctcgctttga aggcgataaa gtcccaatga tgacgctact
    39001 gtcagatttg cttctcgcct acaagatggg agttaaaacg ctttactacc acaacaccag
    39061 agatggggca ggaaagcgtg atgacgacga accgcagaat ccactgacgc aagctgtagc
    39121 cgtcgagcca gaagatgagt gcgacggagc ctgcaaaatc tgacatatgg tggggtgtat
    39181 ccccaccttc tctttgattt gtaagccttg tttaaacaaa taggataaca acttgtttaa
    39241 acacatcaaa aagcaaaagg aaaaacacat gtcatattca acgttccgtt tgggtgctaa
    39301 tgatgcaacc aaagagccta tgttcctcgg acaatctgtc aacgtggcac gttacgatca
    39361 gcaaaaatac cgtgattttg aaaagttgat tgaacgtcaa ttgtctttct tctggcggcc
    39421 ggaagaagtt gatatttcga gcgatcgtat cgacttcaac acgaagctgc gggaccacga
    39481 acgtcacatt tttctgagca atctccgtta tcaaacgtta ctcgattcag ttcagggacg
    39541 tagcccaaat gcaacgctgc tgccgcttat ctctattcct gaactggaaa cgtgggttga
    39601 aacatggtct ttctctgaga ctatccatag ccgcagctac acccacatta ttcgtggcat
    39661 ggtggacgat ccgagcattg tttttgacgg tattgttacg gatgaagaaa tcatcaaccg
    39721 agcgatcagt atctctgctg aatatgacag gctttatggg atgacctgcg agcgccagtc
    39781 gttaggcgag aaggaatttg agcgtctgta cgtaaatgaa tatggctggg agccatatcc
    39841 tttgcaccgt cagcttttcc gcacgttggt gtccattaat gcgcttgagg cgatccgttt
    39901 ctatgtaagt tttgcatgta cgtttgcctt tggcgaacgg aagttgcttg agggtaacac
    39961 caaaattatg cgctttattg cccgtgatga agctctgcat tgcgaaggaa ctgaacgcat
    40021 gatccgcttc atgcgtaccg gtcgcgaagg tttattgtgg aaagagattg ctgctgatga
    40081 agaaaacgtc atttacgaca ccatgaaatc agtcgccgaa caagaaatga actgggccga
    40141 gtatctcttc aaagacggtt cgatgattgg tttaaacgcg gatattttga agacctatgt
    40201 aaaataccgc accaatctgg ctatgaatcg tcttggcctg aaggctttat ttccagaggt
    40261 taccaccgat ccgctggtct ggatgaacaa gtggttgtta accgacacac tgcaaattgc
    40321 accacaagag gcagagcaaa gcacatatct ggtaggtcag atcgattcta ccgtggacaa
    40381 ggcttctcta agccagtttg cagacctgtg aaccgataca aagcattatg tgacctggca
    40441 acgctgggcc acaatggatc acaagaatta agaaggaaca aaactagcat gaactttacc
    40501 aaactgactg accacctgaa acttgccacc gatcgtctca ttggatttaa gccagaacca
    40561 tatgagttgc atgaaggtca tggtgtagct actgaaagta tttacaagat ggtcgatcag
    40621 tttcatgaac tcttccagca tccgagacgc gttatgccga caccagagct gcttcgtctc
    40681 cgtgcaagcc tgattcatga agaagctgta gtggaaggta ttccagctgc aatgaatggg
    40741 gatattgagc aactgctgga tgcaatggcc gactttttat acgttggtgt tggtacgatg
    40801 gtcgccatca aaggtggtat ttctaccggc atgacctatt acacgcagga acagagcatt
    40861 gatcgcttta tgcagacaat ttttgtgccc ggtaacactg ttttcgatga tatggcaatg
    40921 ccatttcagg aagctcgtga ggcgtcatgt atgctcgaag agctggcaga taaacttgag
    40981 aacaagactg ttaaggattc tgagctgatt caggaactgc gccgtgtcat gaacaaaatc
    41041 tatgtggcgt gcatgatgac ctatcgactg gctgatttcc tcggtatcaa tgtagtcgag
    41101 ctggttggcg aaattcatcg gtctaacatg acaaaattat ggcctgctga tgtagaggaa
    41161 cgtcgccagg ctgtggccaa ctgcaaatac gactcttcag acctgggatt tcgccatgct
    41221 gatggcaccg ataagatgat cggttttcga atttccgatg gaaagattct gaagtctcca
    41281 acctatagtg atgtcgattt atcctccttt gttgagcaag ctaaagcctc agcaatgtac
    41341 ggaatgatca aaaaataatt gtaggtgatt atctactaat gtatatttga tttgcgtata
    41401 aaactctgga acgactattc tttttggtgg cctatggcca ccatttttta tctatctggt
    41461 cttgttttat caatgaatgt aaactcacgt gataaataat tgattactta tcttcgtgag
    41521 gttttatgtc actccttttg aatcgtgagc atacgaacgg tcaggtaaca aacgcatcgt
    41581 atgcaaaagt tattgagacg gtgcttaaaa gcggcgtgca gactgatgat cgcacaggca
    41641 ctggtacttt aagcacctgc tacgttccct cttactacat gcttactggt gggactgtgc
    41701 cgcttatttc tggaaaggcg gtaaatctta agccactgct tgtcgaactt gagtggtatc
    41761 tgaaaggcac gggcaacatc caatttctta aggataacgg cgttaagatt tgggatgcat
    41821 gggccgatga gaatggcgat ttggggccgg tttacggtaa gcagtggcgt cgatgggaag
    41881 atacccgcat cgtgagccat agtgaatatc tgagcaagat cgctactttc cgtgaacgcg
    41941 ggtacaaagt cgagggatac ctgggtatca gtgaagatcg cgtagtgctg tcccgtgaaa
    42001 tcgatcagct acagcgtatt gtcgacacac tgcgcacgaa ccctaccgat cgtcgcatca
    42061 tgcttaacgc atggaacgta ggcgagcttg aggatatgaa actgccacct tgccactttg
    42121 tcttctcttt gtggagtcgt gagcttgatt ttgaaacccg tttaacgatg gcaactgaca
    42181 ttggtcttca acacagtcgc ttcggttatg agtctatcta caccaagatg ctatacgatc
    42241 tggagatgga cggcagtgtt actgaaactg aactggatga acttggaatc cccaaacgca
    42301 tcctcaactc ctgcctcgta cagcgtagcg tagacacttt tgttggtatg ccattcaata
    42361 ttgctggcta tggcattctc actcattttc tcgcgaagat tacgggtcac atggccggtg
    42421 catttgtgca ttttggcttt gacgtgcatt tgtacaacaa ccacatggaa ggtgtgtgtg
    42481 agctaatgaa acgacaggct ccagagcatt cagatccggt cgttattttc cctcatgaat
    42541 ggtctgagct ggatgatttc aaatgggacg atattctgat tcttggctat gaccctctac
    42601 cgtggatcaa ggttccagtg gcggtgtgat atggcaagag gtatgtatgt cttatgcgaa
    42661 attgaaggtg tgctggcaaa taccagccat cgtaaatctg tacctgacac ggatgcaggc
    42721 cagctcattg ccggtgatga actcattttc cccaccagcc gtatgttgcg tggttttgct
    42781 cgctcagggg ctgaagtggt gcttatcagt agccgctctg aaactcttga agcgcccact
    42841 aaacgatggc tgaaagattt tggcgttgat tacgactggc ttcatctcgt accgaatggc
    42901 accagttatg agaagcatat taagcgcaca ttagcggagc ataaaggcga tctgcttatc
    42961 gctgcgctgg tgcacgatcc tcgactccgt gccgctttag ctgactctca tcatagaccg
    43021 gtcatctatg aggtgagcaa atgaagatga tagctgctgt tggccgtaac tatgagatcg
    43081 gcatagcgaa cgaactgccc tggcgttgtt ctaccgatct gaagctattt aagagactca
    43141 ccaaaaacgc cactgtcgtt atggggcgta aaacgatgga aagtctcaaa cgccctcttc
    43201 cagagcgtca taacctcgtt ttgacgcgct ctcatggctt tgtaccaaat ggattctacc
    43261 ctgctggtgt ggatgatgtg ttgcgattac cagagcctgt gtgggtgatt ggcggggaac
    43321 aaatttactc gctattcatg ccgcatgttg aagagatttg gctctcccac atcggcgttg
    43381 atgtgccaaa cgccgatgca ttcttcccgg caagcatgat gcgtaattta ggctttgtgc
    43441 ctgttgaaac agtttttacc caacgagcca gcgaggaaga gcctggcttt tcgcagatcg
    43501 tatacagaag gtcgtaatgg attaccggat tgggatcact ggtgctcagg gcagtgggaa
    43561 aacaaccctg gcaaaatata tcgacaaaca ttacggaatc ccttacgtgg atgctggtgt
    43621 cggaagtttg atgagccgac tcggtgttcg agtaggtgag tctatgcctc tatatgagcg
    43681 gcttcagatt caaatggaaa tcgcaaagca tatagagcta cttacgcgtg gtgttgaagg
    43741 ctttgttatc gatcgcacac ctgctgatgt tatggcctac acgttggatt tggtcggcca
    43801 taccaatgaa gatcgatgta ttgagttagc cctcgatatc gaaaagtttt gccacaaaac
    43861 tgctatttca aactttaacg ccattgctgg cctacgcccg ggagtcgctc tctcagagcg
    43921 agattacttg cgatcacaac gagcatcatt agaccgtctg tatgtcgctc gtattgatgc
    43981 gttgatgtgc ggggaactga caaaaattca cctgcatccg caaagggggg atctgcaaac
    44041 cttcgtcgtt tccaaccgat atcgcacggt tgaagcaaga gccagatcag tgatgagaat
    44101 gctagataac gctgtagaaa agatagaaaa ccggttctgt ggtcgagtga ccgttcatta
    44161 gaaattgttc gcctcttcga cattgcgaca ataaaactct caaaatgggt taaggataaa
    44221 aaatgtttag tgaaatgttg cttgaagatg aactggatcg gaaaacaaca gaggctttga
    44281 ttcgtgtagc ggacgaacat tcccggtcgc ttatgagcga tcgagaggct cgtctggcta
    44341 ttcgtgccat attcgaaact gcgcaggggc ttgttggcgc acaagtaggt gaagccatta
    44401 acatcgccat gtctcagttc agtgaagaca gtaaaaagcc tctgtttcct atgcatttga
    44461 tgctggctgg tggcacggtg ctttatatct ctgtttgtct ggatagcaac caaatcaata
    44521 ttctcaacac tgcgtcaggt aagtggaaag atccgattgt ctgtgaaacc agtgaagaaa
    44581 ctttgaaaaa agcggctcaa tttgtacgta gcgcactact taagggcgct aagaagttgt
    44641 aaggagttct gatgacaacg attgttgcag gcatcgatat cgagtctacg ggactggatt
    44701 tccttgctgg tcataaaatt attgaaatcg caattacccg ctatgaactg gagacacaga
    44761 gacatattga tagtctggag atgcgtttta accctcgcag aaacatagat ccgaaagctc
    44821 aagccgttca tggcatttca ttagaacagc tcgcagctga acctttgttg tcaaatcatg
    44881 ctagcgaaat tggcgcttat atggaggcat gtagtgcgtg ggttgctcat aacggcgaag
    44941 cgtttgatat accatttatt agacacgagt tttcagggta tggagtaaga ctgccagacg
    45001 ttcccgttat agatactatg ttatcgggat tgtgggccac agaagacggt aaacgtcctc
    45061 gccttgaaga attggccttc tctcttggct ttatatacga tcatgccaaa gcacatagtg
    45121 ccttatatga cacaaactta atgatgcaat gcttctttaa ggcacgtaat aaatacggat
    45181 tttttaaatt accctctgaa attgtgtaaa acaaaagcct gctttaaaga gagtttgaag
    45241 caggctttct tttaaagaac agtcgccttt caatcatttc ctgcctgtat ttaatacttt
    45301 tccgcctgat aggtttagtc aaaatgtagc catcgaaacg caaatgcaac taaacagaag
    45361 gagacttaca tgagttcggt tgaaaatgta atgacaaatg atgatctgga tgagctgaca
    45421 gccatgttgc aatcacttga tgaaccagta aaaaaagctg cacaggttga aaatactgat
    45481 gatattgacg atctgcttct cggcatcgat gctggcgtag ccatgagttc tgatgatgtt
    45541 gccgaagaac tgttcaatga agaaaaagca ggtgatttca gctctgcttt aaatgagttg
    45601 gagttagcgc atgagcctat aaacgtaatt aacgctgaaa gtggtgaagc tgccgaaaat
    45661 gagccagaac agttggggat cattgaggtt gaagattgtg ttgaggttaa tgatgaatta
    45721 aaagttcaac agtcaaatga tagcaataca aataaaaaaa ctcgtgctgc aagaggtcct
    45781 agttttactt taagtgataa agatgattcg tttttcaata aagcaggctt agaaaaagat
    45841 attttcttag acgcttacga aaacgcgcct gtcaaagcga aggataagat attaaacctt
    45901 cttaattggt ttagcggagg tccagatatt agtgtttaca cggtaatttc catgagacac
    45961 cttcttacag aaaagaaggc tacaagtaat agtattaaga ttgctttaat gagcaatcca
    46021 gaaaaaccgt atccgcttaa cactgcgtca actcaggctg ggcaaatgat ggctgtattt
    46081 ccagctacag gaattgccgt tagagacggt ggaaatctaa cattgaacga agaatcaccg
    46141 atcgttaaga agtttgtcgc ggagtacact attggatgac gttcccctac tgaaaataaa
    46201 gcccatagag agctttatag tgctgggtaa gataacccat acccagcacc aaaaaaacgc
    46261 gccagagagc ttcttgttcg catttctggc gcgttttatt tgattgccat acataaaatc
    46321 aaatgcaaaa ataggtattt acttacctat cgagaaagaa gatgattgca gccgaaaaaa
    46381 tcaaacagcg aaagcgcgac aactctcttc gtgacctctg gagaacacct gattggctgt
    46441 tttctgccat tcaacgttat cttggagtga catttgatgt tgacgttgcc tgcaacaagg
    46501 acaacgcaaa gctgcctaat ttcataggca ttgagcgtga tgctttgaaa tctgaatggg
    46561 gacagccagg tacaattgcc ttcctcaatc caccctactc caaaatctcc ccctggattg
    46621 atgctgctat acgtgagcag gctcgcggag ttactacagt gatgctaatt cctcaatccc
    46681 tcgatacaaa gtggtatgag cgtgcaacag agtatgcgaa tgagacgatt attctgtctg
    46741 gtggccgcgt agcgtttgtc gagcctgacg tcaatttggg tcaggtagaa gtaaacatca
    46801 accccggtgg cagtatgctc gttgtttttc gaggattctg tcaggacgct gggcactcta
    46861 taagcaagat ccctttggac gtcatgaaaa gtctgggagg gtatgatcct gcgaatgtga
    46921 tcaggaaaaa aagaccatca aagaaggctg cttagtttgt tctggcgtct gtaattagcc
    46981 tgcttctgta tatataaata actacatatt aattattaat atacggaagc aggctgtttt
    47041 gtatcagaga ctccagacct gaacatcact acagaatcca ctagaccccc cttcccagac
    47101 gctttaaaat cgattttatg aaccacttta aggaaaccaa catgtcatac ccgactaatg
    47161 tcgttgcgct cgtagacagc gattttctgg cccaggctcg tgaaatgatg aaagatcgtg
    47221 agcaggcttt caacttgtac gagtgggcaa ttaagtgctt gcatcttggg gagcatcgcg
    47281 aacttgttga acagctttta ggtgagttga tcaacgaggt gtttgccttg aatgttcaac
    47341 tacatggtcg agaaaataat caatcgaaat gatagataag tacaaactat tcataagatg
    47401 aattgtaagt gctaagatct gatagtttcc agtcgtagac tggagactcg acctgatggg
    47461 tgggggtaag cgtcactggc gtcaggttta aaaaagctca ctaccagcgt agaaccggtg
    47521 ccgtttaggt gtcggggaag ggggaaccaa agtgagcgga gacaagggtc actttatgat
    47581 tgtcgagtct ggggtgtttc gagaggttga atccagtact ccccttcata aagtgtggga
    47641 agatctcggt tctggggtgc tgtcatccat aacttcccaa gtctaagctg gcagtagact
    47701 tagaccatag cttttcaggt tatgaaacga ccaggttggt gaggaatttt atactcacct
    47761 ccctgggaga gtattacctg aaaagacaac ctctcacttc gttcgaggtg aacttcactc
    47821 acttcgttcg ttcagttcag gtttataaaa acctgttctg ggaa
//
LOCUS       DAZPAC010000006        45923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZPAC010000006 DAZPAC010000000
VERSION     DAZPAC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45923
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.006"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..716)
                     /locus_tag="ACHUPM_000270"
     CDS             complement(<1..716)
                     /locus_tag="ACHUPM_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0868168.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHUPM_000271"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHUPM_000271"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR0868169.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHUPM_000272"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHUPM_000272"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR0868170.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="ACHUPM_000273"
     CDS             complement(3650..4705)
                     /locus_tag="ACHUPM_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0868171.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="ACHUPM_000274"
     CDS             complement(4715..5503)
                     /locus_tag="ACHUPM_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR0868172.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="ACHUPM_000275"
     CDS             complement(5521..6573)
                     /locus_tag="ACHUPM_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR0868173.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7207)
                     /locus_tag="ACHUPM_000276"
                     /pseudo
     CDS             complement(6604..7207)
                     /locus_tag="ACHUPM_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(7194..8075)
                     /locus_tag="ACHUPM_000277"
     CDS             complement(7194..8075)
                     /locus_tag="ACHUPM_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR0868174.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8096..9406)
                     /locus_tag="ACHUPM_000278"
     CDS             complement(8096..9406)
                     /locus_tag="ACHUPM_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0868175.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIVLPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9441..10951)
                     /locus_tag="ACHUPM_000279"
                     /pseudo
     CDS             complement(9441..10951)
                     /locus_tag="ACHUPM_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(11163..11477)
                     /gene="pspE"
                     /locus_tag="ACHUPM_000280"
     CDS             complement(11163..11477)
                     /gene="pspE"
                     /locus_tag="ACHUPM_000280"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR0868176.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11552..11773)
                     /gene="pspD"
                     /locus_tag="ACHUPM_000281"
     CDS             complement(11552..11773)
                     /gene="pspD"
                     /locus_tag="ACHUPM_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR0868177.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11782..12141)
                     /gene="pspC"
                     /locus_tag="ACHUPM_000282"
     CDS             complement(11782..12141)
                     /gene="pspC"
                     /locus_tag="ACHUPM_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR0868178.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMLDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12141..12365)
                     /gene="pspB"
                     /locus_tag="ACHUPM_000283"
     CDS             complement(12141..12365)
                     /gene="pspB"
                     /locus_tag="ACHUPM_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR0868179.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12419..13087)
                     /gene="pspA"
                     /locus_tag="ACHUPM_000284"
     CDS             complement(12419..13087)
                     /gene="pspA"
                     /locus_tag="ACHUPM_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR0868180.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13254..14231
                     /gene="pspF"
                     /locus_tag="ACHUPM_000285"
     CDS             13254..14231
                     /gene="pspF"
                     /locus_tag="ACHUPM_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR0868181.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14349..15614)
                     /gene="puuE"
                     /locus_tag="ACHUPM_000286"
                     /pseudo
     CDS             complement(14349..15614)
                     /gene="puuE"
                     /locus_tag="ACHUPM_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15652..16932)
                     /gene="puuB"
                     /locus_tag="ACHUPM_000287"
     CDS             complement(15652..16932)
                     /gene="puuB"
                     /locus_tag="ACHUPM_000287"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR0868182.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16934..18424)
                     /gene="puuC"
                     /locus_tag="ACHUPM_000288"
                     /pseudo
     CDS             complement(16934..18424)
                     /gene="puuC"
                     /locus_tag="ACHUPM_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(18574..19131)
                     /gene="puuR"
                     /locus_tag="ACHUPM_000289"
     CDS             complement(18574..19131)
                     /gene="puuR"
                     /locus_tag="ACHUPM_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR0868183.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19158..19922)
                     /gene="puuD"
                     /locus_tag="ACHUPM_000290"
     CDS             complement(19158..19922)
                     /gene="puuD"
                     /locus_tag="ACHUPM_000290"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR0868184.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20134..21495
                     /gene="puuA"
                     /locus_tag="ACHUPM_000291"
     CDS             20134..21495
                     /gene="puuA"
                     /locus_tag="ACHUPM_000291"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR0868185.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21644..21808
                     /locus_tag="ACHUPM_000292"
     CDS             21644..21808
                     /locus_tag="ACHUPM_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868186.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21798..23183
                     /gene="puuP"
                     /locus_tag="ACHUPM_000293"
     CDS             21798..23183
                     /gene="puuP"
                     /locus_tag="ACHUPM_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR0868187.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23317..23562
                     /locus_tag="ACHUPM_000294"
     CDS             23317..23562
                     /locus_tag="ACHUPM_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR0868188.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23875..25518
                     /gene="sapA"
                     /locus_tag="ACHUPM_000295"
     CDS             23875..25518
                     /gene="sapA"
                     /locus_tag="ACHUPM_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR0868189.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25515..26480
                     /gene="sapB"
                     /locus_tag="ACHUPM_000296"
     CDS             25515..26480
                     /gene="sapB"
                     /locus_tag="ACHUPM_000296"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR0868190.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26467..27357
                     /gene="sapC"
                     /locus_tag="ACHUPM_000297"
     CDS             26467..27357
                     /gene="sapC"
                     /locus_tag="ACHUPM_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR0868191.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27357..28349
                     /gene="sapD"
                     /locus_tag="ACHUPM_000298"
     CDS             27357..28349
                     /gene="sapD"
                     /locus_tag="ACHUPM_000298"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR0868192.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28351..29157
                     /gene="sapF"
                     /locus_tag="ACHUPM_000299"
     CDS             28351..29157
                     /gene="sapF"
                     /locus_tag="ACHUPM_000299"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR0868193.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29225..29578
                     /locus_tag="ACHUPM_000300"
     CDS             29225..29578
                     /locus_tag="ACHUPM_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR0868194.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29946..30734
                     /gene="fabI"
                     /locus_tag="ACHUPM_000301"
     CDS             29946..30734
                     /gene="fabI"
                     /locus_tag="ACHUPM_000301"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR0868195.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30879..32005
                     /locus_tag="ACHUPM_000302"
                     /pseudo
     CDS             30879..32005
                     /locus_tag="ACHUPM_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32073..34007
                     /gene="rnb"
                     /locus_tag="ACHUPM_000303"
     CDS             32073..34007
                     /gene="rnb"
                     /locus_tag="ACHUPM_000303"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR0868196.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34085..34188)
                     /locus_tag="ACHUPM_000304"
                     /pseudo
     CDS             complement(34085..34188)
                     /locus_tag="ACHUPM_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34242..36232
                     /gene="pdeR"
                     /locus_tag="ACHUPM_000305"
                     /pseudo
     CDS             34242..36232
                     /gene="pdeR"
                     /locus_tag="ACHUPM_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(36214..36342)
                     /gene="ymiD"
                     /locus_tag="ACHUPM_000306"
     CDS             complement(36214..36342)
                     /gene="ymiD"
                     /locus_tag="ACHUPM_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR0868197.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            36379..36552
                     /gene="yciZ"
                     /locus_tag="ACHUPM_000307"
     CDS             36379..36552
                     /gene="yciZ"
                     /locus_tag="ACHUPM_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR0868198.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36642..37391
                     /locus_tag="ACHUPM_000308"
     CDS             36642..37391
                     /locus_tag="ACHUPM_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR0868199.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37660..37878
                     /gene="osmB"
                     /locus_tag="ACHUPM_000309"
     CDS             37660..37878
                     /gene="osmB"
                     /locus_tag="ACHUPM_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR0868200.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38004..38330)
                     /gene="yciH"
                     /locus_tag="ACHUPM_000310"
     CDS             complement(38004..38330)
                     /gene="yciH"
                     /locus_tag="ACHUPM_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR0868201.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38330..39067)
                     /gene="pyrF"
                     /locus_tag="ACHUPM_000311"
     CDS             complement(38330..39067)
                     /gene="pyrF"
                     /locus_tag="ACHUPM_000311"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR0868202.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39260..40429)
                     /gene="lapB"
                     /locus_tag="ACHUPM_000312"
     CDS             complement(39260..40429)
                     /gene="lapB"
                     /locus_tag="ACHUPM_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR0868203.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40436..40744)
                     /gene="lapA"
                     /locus_tag="ACHUPM_000313"
     CDS             complement(40436..40744)
                     /gene="lapA"
                     /locus_tag="ACHUPM_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR0868204.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(40893..41657)
                     /gene="pgpB"
                     /locus_tag="ACHUPM_000314"
     CDS             complement(40893..41657)
                     /gene="pgpB"
                     /locus_tag="ACHUPM_000314"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR0868205.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            41827..42417
                     /gene="ribA"
                     /locus_tag="ACHUPM_000315"
     CDS             41827..42417
                     /gene="ribA"
                     /locus_tag="ACHUPM_000315"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR0868206.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(42481..45156)
                     /gene="acnA"
                     /locus_tag="ACHUPM_000316"
     CDS             complement(42481..45156)
                     /gene="acnA"
                     /locus_tag="ACHUPM_000316"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR0868207.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45320..45415)
                     /gene="ymiC"
                     /locus_tag="ACHUPM_000317"
     CDS             complement(45320..45415)
                     /gene="ymiC"
                     /locus_tag="ACHUPM_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR0868208.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45529..45696)
                     /gene="yciX"
                     /locus_tag="ACHUPM_000318"
     CDS             complement(45529..45696)
                     /gene="yciX"
                     /locus_tag="ACHUPM_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR0868209.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45699..45839)
                     /locus_tag="ACHUPM_000319"
     CDS             complement(45699..45839)
                     /locus_tag="ACHUPM_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR0868210.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT        10848 a        12140 c        11560 g        11375 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac aggcagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccgag atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttacctcttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatcgc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cacttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc agcccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtcgac gtaatgctcc agcgtccact gctgcggcag cagcgtagga tgcagtgaga
     7081 tcgcctcttt cgcgcccttg aacgaggtca tcagcatcac aaaaaatgga aacagcgtga
     7141 tgatgagaaa cagcgccagc ccgcagtaaa aaccgatgcg actgagtgtg cgtttatttg
     7201 ttgccattga ggttcacccg tttcctggtc agcagaatga cagcgaaaat aatgatgaag
     7261 agcaccaccg aaatcgccgc cgctttccct aagtcattaa aggcaaaggc ggttttgtag
     7321 agatagacgc cgagaatatc gaccttggtg gtcagcagat aaacatcagc aaacatgtag
     7381 aacatccaga tagtgcgcag ggtcaccacc gtcgccagga ccggcataat tgcgggcagc
     7441 gtgacgatgc gaaaccgctg ccaggcatta gcgccatcca tctctgccgc ttcgtacagc
     7501 gatttgtcga tggtctgcaa aatcgccaga aacgagataa aggcatacgg gaagtagcgc
     7561 cagatggcga acagcaccac cagcacaaag ctactgcccg gattatcgaa ccacagcggt
     7621 gcctgctcat agagatgcag aagatcgacg ccgaggtagt tcacaatgcc gtagccgttg
     7681 ttgaacatgt atttccaggc aaacaccagc gaaatggacg gcgttacgta ggagaggatc
     7741 accagcgaac gcgcggtttt gcgcaggcgg aactcgcggt tgaaaaacat cgccactgcc
     7801 agcccgagca cggtgctgcc cgccaccacc agcgcggtat accagaccgt catccacagc
     7861 gagtgccaga agccgggatc ggagaggata cgcacatagt tactcacccc aacaaaggtt
     7921 gactcgatgt tgggattgag cggcagacgt aaaaaactga tttcgatatt cgacaccatt
     7981 ggccaggcca ccagaccacc cagcagtaat aagctgggcg cgagaagcag cagcgcaaag
     8041 ggcatatcgg aacgacctga aaaaagcctg ttcattgcgg attcctgtcc tgggtttagt
     8101 gctgttcgat cagttcatcc agctttttct ggctcgcttg cagcgtgcca gggagatcgg
     8161 ctttacccac ggtgacgtta tgcaccatgg agctcaccac gccagaaccc gtcacatcac
     8221 ccatgcgggt aaagttttta tcccctactg cgccaaagac ctgaatattt ggcagttcac
     8281 cgattagctg attcggtagt tcacccagcg ccttaataac gtcgttgtct ttccaggtgg
     8341 cggtagtgac aaccgcttta ttcaccggca gcgcagcacc tggcgacatc atcacccagt
     8401 cggcaatgtt gtctgcctgc tccataaagg tgacaaattt ttctgctgct tccgtctctt
     8461 cggccttttg cccggcggta atggtcagcg aggtcaacat gccgtagacc gcagagtttt
     8521 tctcggttgg caccacgaaa ccgacgtttt tcgggtcgcc ttctttaatt acagccggaa
     8581 ggatataggt ggagtaaatc gccatcggcg cggtgccgtt cataaaggcg tctttcactt
     8641 ccatgatgtc gttagaaccc ggcatgtcgt tagaacccgg catagtgttg gcagcaaggt
     8701 cgcggtaata ggtcagtgcc tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg
     8761 cgttaaagac attagcctgg ttggataacg caaactggga gaaggattgt tccgtcaaca
     8821 cgctttctgc tgtaggcagc acaatgccgt attttttatt cgccgggtca ttcagtttct
     8881 gtgcaacgtc cagtagctgt tgccagtttt tcggctcctc cagccccgct tttgccagca
     8941 catctttgcg ataccagatg ccgccaatcc aggcgctgac gggaacaccg gtccaggcac
     9001 tcccatcttc ggtgcgcaca atacgcagta cgccatcgta aaacgcgcct tcaccaacgt
     9061 tgctgatgac cgtggcaacc gtcttgcgat cgataagctg ctctttatcc ataactttgg
     9121 cgtagtcatg gctggtttcg atcacttccg gcagcgagcc gctacgtgaa agggtgatga
     9181 ctttggtgtt ataagcgtcc tcttccacgg gcacttgttt aacggtgata cctgggtttt
     9241 ctttttcaaa acgggcaatc aatttgctga taactgcctg acgctcctgc tccaccgaag
     9301 aatgcataaa ttcgatggat acatgggttt tattttcttc tttacagcct gaaatcaggg
     9361 cgcaggaaac cagtgctgat aacagcacga tttttgattt aatcatgaga tgttcctttt
     9421 tatttaatcc acataacctg ttaggggcgc agcgttattt cattacccaa tagatcaact
     9481 tcactaatca gatcccgacc atgcagatta gtaatattta catgctgaat atttttactg
     9541 acattaaaca atccagtcag acaatcacca tcagcgttac ttctttgaat acgcattacg
     9601 gatgaattaa tcgtatcgat ggtaaaatta ttatccggat gaaactcgtt atggctgcga
     9661 cgaagtgtaa ttaaacgcga caactcatga tataccgcat gccttaatgt agcttcatcg
     9721 ttcagttctc gggttatctc tttactgtga tattttttac ggttaatcgc acggttatat
     9781 ccgagttttt cgacacctgc gtaatcatta cgcgagccaa gaatactttg aatatatatc
     9841 gctggcacac cggggaaact taacaaaatc gcatgggcga gaataaacct ggcgcaacgt
     9901 tcttcatcgc tactctcacg gcggcttaac gcatccatat gtgagaggcg aggaagttaa
     9961 accaggtggt gttgctggaa ggtagtgtca ggttttgcgc ccacgcacaa agcgcctcaa
    10021 cgttctgttt ttgcaccgca tgcagcacca gtggcggcag cgagaactgg tacaccatat
    10081 gtgcttcgtc atcgcctgcg ccaaagtaag caatgttgtc tttatgcgga acattggtct
    10141 cggtaatgat cactgtacct ggcgcaacgt tatcaataat cgaccgtaac agtttgataa
    10201 tcagatgtgt tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa
    10261 cggcatccag gcggacatac tcagcacctt tcgcaaggta acagagtaaa acatccacca
    10321 tcgccagcaa cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg
    10381 tccataaatg gcgcgttgaa tgatcgcgca tctggaatgg cgttaataac ggtaacgcac
    10441 gcgggcgagt gacggcgctg agatcggttt gcggatcaac cgcaataaaa aaatcttcaa
    10501 aacctggctg ctgttgtaaa tagtttttaa accattcact ttttgccgac atatggttgc
    10561 agacaaaatc aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc
    10621 ccgcttcact ggcgacctga tgataatcaa ttaccgaaaa cccatcatca gatgaacatg
    10681 gataaaaagg taataaatga acatgtgaaa aaatgctttg cagccattga tggtaaaact
    10741 gattaaatgt gggtaatggt tttaaatcat tgctgtgaaa ttgatcggca taggtaatta
    10801 acacgacatc actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac
    10861 tctcaagacg cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt
    10921 cgtccaggta atccgtaatt ttctgtttca tgttgccttt ttgtggtagc ggtcccatga
    10981 aacgagagac tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc
    11041 aggaaatcat tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatgg
    11101 ggatgtgaat aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc
    11161 ttttaacctt tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg
    11221 gtatatccca tctcgctaag gatctctttt gcttgccctg actggcgtcc ggcattgcaa
    11281 tacactttca cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc
    11341 ttcaggggaa tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga
    11401 acatcgatcc agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt
    11461 aagccttttt taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc
    11521 ctgagattta acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat
    11581 tagcagcccg acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc
    11641 gagcaactga ttttatcgcc cagcccgcta cacccgccgg gccatagcgc agtgcggtaa
    11701 gaagtaccag cttgcctgct aatttgaaac caggctttac cttttgcccg gcctgttgcc
    11761 agcgagtatt cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc
    11821 ggaagtgaca taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc
    11881 gacttcatcc agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccag
    11941 cattggatca agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc
    12001 gaagaaaatc gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt
    12061 ggcaatcccg gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataatttttt
    12121 attgagatta atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata
    12181 gattccagcg cctgaatacg ttcgcgcatc cgttttgctt catcagccag ttgcgctaat
    12241 cgctgctgct cactttgcga caattcatcg cgaccagaac gattgctgta atgcagccat
    12301 aaccagatcg gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg
    12361 ctcataagta ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt
    12421 attgattgtc ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat
    12481 cggctttcag ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt
    12541 ctgcttccgc ttccatctgg tcaattcgac gttcgaaaga ttcgaaacga gccattgctt
    12601 catccagttt gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat
    12661 gacgtaacat caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc
    12721 caatctcttt cttcatgcgt gccagcgtat cgttcaccag cgtcacttca tgttccaggg
    12781 acttaatcag atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc
    12841 tctctttcag cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt
    12901 gttcaatacg gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta
    12961 cttcaaccag tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt
    13021 ccgctttctc taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa
    13081 tacccataat gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata
    13141 caatttgcgt gccaattttt tatctttttg atttataaag atctgattga agaatcaaca
    13201 gcaacatgcc aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg
    13261 aatacaaaga taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt
    13321 cgcatctcgc accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag
    13381 agctgattgc cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc
    13441 ttaactgcgc ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg
    13501 gggcgtttac cggtgcgcaa aaacgtcatc cagggagatt tgaacgtgcc gacggcggta
    13561 cgctatttct tgatgaactc gctacggcac cgatgatggt gcaggagaaa ttattgcgcg
    13621 tgattgagta cggtgaactg gagcgcgttg gcggcagcca gccattgcag gtgaatgtgc
    13681 ggttggtatg cgcgacgaat gccgatctcc cggcgatggt caatgaaggc acttttcgcg
    13741 ctgacctgct cgaccgactg gcttttgatg ttgtacaact gccaccactg cgcgagcgcg
    13801 aaagcgacat aatgttgatg gcagaacact ttgccatcca gatgtgtcgg gaaatcaagc
    13861 tgcctctgtt cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc
    13921 cgggaaatat tcgtgaattg aaaaacgcgg tggaacgttc agtgtatcgc cacggcacca
    13981 gcgattatcc gcttgatgac atcattattg atccctttaa acggcgtccg cctgaagacg
    14041 ctatcgccgt ttcagaaacc acctcgcttc cgacgctgcc gctggattta cgtgagtttc
    14101 aaatgcagca ggaaaaagag ttgctgcaac tcagtttgca aaaggggaaa tataaccaga
    14161 agcgcgcagc tgaattgctg gggttaacct accatcagtt ccgcgcgttg ttgaaaaaac
    14221 accagattta gcgaagttgg gcaatgcgat tcgccggatg cggcgtgaac gctttatccg
    14281 gcatacataa tcaacgtcgt ttgcaggcct gataagcgca gcgcatcaga cattattgcg
    14341 ttgggctatt aatcgctcag cgcatcctgc aaaattttca ttgccgcatc gaattgcgca
    14401 tccgggatgg tcagcggata caggaagcga atcacgttgc cgtatgcgcc acaggtcagc
    14461 aggagcagcc cctgcgccag cgcgcgttgc tggattttct gtgcaatcgc cgctgacggc
    14521 tcgcccgttt gcggatcgtt aaactctgcc gcaatcatcg accccaggcc gcgtaccgca
    14581 gcaatggccg gaacgctttc tttggcatca atcaacgtgt ttttgagacg ctggcccagt
    14641 tgattcgcgc gttcgcagag tgattctttg ttgataatat tgagcaccgc gtgcgcggca
    14701 gccaccgcca gcgggttccc ggcgtaggtg ccacccaacc cgcccggcgc gggcgcgtcc
    14761 ataatattcg cgttaccgac cacgcccgaa agcggcatcc cgcccgcgag gcttttcgcc
    14821 atcgtcatta agtccggctt atcggcgtaa tgatccatgg caaacagttt accggtacgc
    14881 gcaaagccgc tttgcacttc atcggcaatc atcacaatac cgtgctcgtc gcacaggcgg
    14941 cgaatggcgg caaccagctc ttttggcgca acgttgaaac cgccctcgcc ctgcaccggt
    15001 tcgaaaataa tggccgcgac ctgcttcgct tcgatgtctg atttaaacaa gcgttcgatg
    15061 gcgtcgaggg agtcctatgt tgaaatgccg tgtaaatctg acggataagg tacgtgatac
    15121 accgaaccag ggaacgggcc gaagccgatt ttgtacggcg caacttttcc ggtcagcgcc
    15181 atggtcatat atgtacgacc gtggaagcca ccgctaaacg caatcacgcc agggcgtccg
    15241 gtatgggcgc gagcaatttt caccgcgttt tccaccgctt ccgcaccggt agtgaagaac
    15301 gcggttttgg cctgcccgct caccggggca agggcgttga ttttctccgc cagggtgacg
    15361 tagctttcat acggcacaat ctgatacgcg gtgtgggtaa actgttgcag ttgctgctcc
    15421 accgccgcga ccagatcagg gtggcgatgt ccggtattca gcaccgcaat gcctgcggcg
    15481 aaatcgatgt actcgttgcc ctcaacatcc ttcagagtag cgttttcagc cgactgggcg
    15541 aagaagttac acatcacgcc aaccccgcgt ggggtagcag aaagacgacg ctgatggaat
    15601 tcattgttgc tcatgtttca caccttttga ctcttcactg ttaatcggaa attaaacgcc
    15661 cagacgatcg cgcaggctgt accaggctgc gcccatcgcg gtaaacggta ctcgcagcgt
    15721 gcgcccgccg gggaacgggt aatacggcag attggcgaag gcatcgaaac gttcggcgtc
    15781 gccgcgcagc agttcggcaa tcaaacgtcc ggcgagatga gtacaggtga cgccgtggcc
    15841 gctgtaaccc tgcatgtaat agatgttggt atcaaggcga ccaaactgcg gcatgcgcga
    15901 cagggtcagc aggaagttgc ccgtccagcg gtagtcgatt ttgacgccct tcagttgcgg
    15961 gaaggttttc agcagtttcg gcacaacaag acgctcaacg tcatccgggt cgcgcgcgcc
    16021 gtagaccacg ccgccgccgt acagcaaacg gttgtcggcg gtaagacggt agtaatcaag
    16081 cagataatta cagtcttcca cacagtagtt tttcgggatc agcgaacggg ctaaatcttc
    16141 cgacagccgt tcagtggtga tcacctgcgt gccgcacggc atgctgcgtt tcgccagttc
    16201 cggctctact ttatcgccca gatacgcatt cccggcgaca atcacatact tcgccgtcac
    16261 ctgacctttg gcagtgcgca ccaccgctgg cgtggtgtgc tggatctgcg tcacggcaga
    16321 aagctcatac acgcgcccgc cgttcaggcg gatggcatcg gcttcaccaa tggcgaggtt
    16381 tagcgggtga atatgcccac cgctgtgatc caacagtgcg ccagtgtagc gatcgctggc
    16441 gacctcgcgg cgaatggcgt tagcgtccag cagctccagc tgtttattgc cgtagcgttc
    16501 ccagttctct ttctgctctt cgagtgtggc gagctgttta tcattcatcg ccacaaacag
    16561 gccgccgggg cggtagtcgc agtcaatctg atagcgtttg atacgttcgc ggatgatctc
    16621 gccaccctcg aacatcatgc tgccgagcat acgggcggtg tccatgccgt agcttttttc
    16681 gatcacgtcg atgtcgcggc tgtaggagtt cacaagctgc ccgccattgc gcccgcttgc
    16741 gccaaagccg atgcgtgagg cttcgagaac cactacgtca aagcccgctt ccgccagatg
    16801 cagcgcggag gagagcccgg tatagccgcc gccaaccacg caaacgtcgc aggtgattga
    16861 ctcattcagt gtgtcgaatg gtgcatattt attcgcactg gcggcgtagt aactgctggt
    16921 atgttcggtc atttcaggcc tccaggctta tccagatggt tttcagttca gtgaattttt
    16981 caagggcatg cagggatttg tcgcgaccgt tgccgctctg cttatagccg ccaaacggca
    17041 cggtcatatc gccgtcgttg tagttattga cgaagacgga accggctttc aggcggcggc
    17101 tcatacggtg cgcgcgggag aggtcgcgcg tccataccgc cgcgccaagg ccgtactggc
    17161 tgtcgttggc aagctgtagc gcctggtctt ctgatgtgaa acgcgtgacc accagcaccg
    17221 gaccgaaaat ctcttcgcgg cttaaggacg cattcgggtc aacatccaca aagatggtcg
    17281 ggccgatggc ggcagccagc tcagcgttac gaccatccag cagcagttgc cctttgcttt
    17341 cgccttcacg aataaagcta tggaccgagt cggcgtgggc gcaatcgatt aaggtgccca
    17401 tggtggttac ggtatcaagt ggatggcccg gctgccagtt ttgcgcctgc tgttttaaca
    17461 gggctaagaa ttcatcggcg atgctctctt ccagtaacaa acgcgttccg gcgatgcaca
    17521 cctggccctg gttgtagaag atgcctgcgg cggtggcgct tgccgctttt tgcagatccg
    17581 gacaatcggc gaaaacgatg ttggcgcttt tgccgcccgc ttccagccag acgcgtttca
    17641 tgttgctatc acccgcgtct ttcagcagtt gtttacccgt acgcgtggag ccggtaaagg
    17701 cgatggcatc gatatcgtta tgacgcgaaa gcgcctgccc ggcttcatga ccataaccgc
    17761 tcactacgtt cagcacgcca tccggcagcc ccgcttcttc ttttgccagc ccggcgagac
    17821 gaatcgcact gagtggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca
    17881 gcgccgggcc gagtttccag caagtcagca gcagcgggaa gttccacggc acgatggcgg
    17941 caatcacgcc gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca
    18001 cttcgccata cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg
    18061 gaatatcatc acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg
    18121 ccagctcttc ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt
    18181 tagccggaga agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg
    18241 catggtcgat atcgacgctc ttgccgcggg caattttcgc cagcggtgcc taggtgaccg
    18301 gatcaacggt ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata
    18361 agcggttttc aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat
    18421 tcattatgac tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca
    18481 gagatccccg ggccgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg
    18541 cattgcgctt acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct
    18601 gataattcgg caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat
    18661 ggcataactt tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc
    18721 accttccagt actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt
    18781 gcccggctgg tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac
    18841 cagcttcatt gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac
    18901 ctgcggctca tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac
    18961 cttcagcagc ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact
    19021 gatagcactg tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg
    19081 ctggcggatt tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt
    19141 tccttaaact gtagtggtca gagtcgctgt ttttcagcga tatggtgctg acaagcggtg
    19201 ataaagccct cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc
    19261 cactgtacgc ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc
    19321 ggcgagcgag cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga
    19381 gagtttaccc aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca
    19441 acctgaactt catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga
    19501 tgttccagca attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc
    19561 accagttctt gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca
    19621 ttaattatcg ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt
    19681 tcaccatata ggtgcggctg cacattgctg ggactaccag gaagataaat gccatcgagt
    19741 ttcggcaaaa gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc
    19801 aagccgcctg cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga
    19861 cccttaagcc tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc
    19921 atgctcaatc tcacaaagtg gactaaatta tcgccattac tgcgctttaa atgaccttta
    19981 tgttcaatat tttttcaatc tagcagtggg ttttcatttt tgcaaactca atttaacatt
    20041 tgacaaacat ttagtttgca tacagattcg aatggtggtc attatatttt acgctttgat
    20101 aacgagcgga aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag
    20161 aactttgttc agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc
    20221 tacctggagc gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt
    20281 tgcttccgtg gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat
    20341 ttcccggcct cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg
    20401 ggtcaggaaa tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct
    20461 tctgccgccg atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc
    20521 gctccctttg acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag
    20581 cgcgacgctg aagggaatct gcaaccgccc tgcgcgcctg gcaccgatga ccgcaataca
    20641 caaagccagg tttactccgt tgataacctc aaccacttcg ccgacgtgct caatgatatt
    20701 gatgaactgg cgcagttgca gctgattccg gcagatggcg cggtcgctga ggcctcgccg
    20761 ggtcagtttg aaatcaacct ttaccatact gataacgtgc tggaagcctg cgatgatgcg
    20821 ctggcactaa aacgcctggt gcgtctgatg gcaggaaagc ataagatgca cgccactttt
    20881 atggcgaagc cgtatgaaga gcacgcgggc agcgggatgc atatccatat cagtatgcaa
    20941 aataatcgtg gcgagaatgt gctttctgac acagaaggcg aagattcgcc gctgctgaaa
    21001 aagatgctcg ccgggatgat tgacctgatg ccgtcgtcga tggcgttgct ggcaccaaac
    21061 gtgaactcgt atcgccgctt ccagccggga atgtatgtgc cgacgcaggc gtcgtggggc
    21121 cataacaacc gcaccgtcgc cctgcgtatt ccgtgcggcg accgtcataa tcaccgcgtg
    21181 gaatatcgcg tggcgggtgc cgatgccaac ccatatctgg tgatggcagc gatttttgcc
    21241 ggtattttgc atggccttga taacgagctg ccgttgcagg aagaagtcga aggcaacggg
    21301 ctggaacagg aaggcttacc cttcccgatt cgccagagcg atgccctggg tgagtttatc
    21361 gagaatgatc acctgcgccg ctatttaggc aaacggtttt gccatgtgta tcacgcctgt
    21421 aaaaatgatg agctgttgca gtttgagcgc ctcatcacag aaacagaaat tgagtggatg
    21481 ttgaaaaacg cgtaattccc cttgcggggt tgtttcatcg gcctgagcgg agccaggcct
    21541 gacatttgct ggtaatccag acatttcccg gatgtcgcgt tatggagcgc aggcggcaac
    21601 gggcttccgt taacgattaa gactgtgacg aggaaatgag atgatgccga tgattaaatc
    21661 cccgcatggc gagggaggct gcgtatgcgc ccctcctgct actgactgga ctccgccccc
    21721 tttgcttccc ttgctaaaca ggtttgattt caggtcgaca cgaccgcaaa ccttattacg
    21781 caggggaggc agcaattatg gctattaatt caccactgaa tattgctgcg caacccggca
    21841 aaacccgtct gcgaaaatca ctgaaattgt ggcaggtggt aatgatgggt ctggcctatc
    21901 tcacgccgat gaccgtattt gatacttttg gtattgtctc cggcattagc gacggtcacg
    21961 ttccggcgtc ctatttgctg gcgctggcgg gcgtgctgtt taccgctatc agctacggca
    22021 aactggttcg ccagtttccg gaggccggtt cggcctatac ctacgcgcaa aagtcgatta
    22081 acccgcacgt cggatttatg gtcggctggt catcactgct ggattatctc tttttgccaa
    22141 tgatcaacgt tttgttggcg aaaatctatc tctccgccct cttcccggaa gtgccgccgt
    22201 gggtgtgggt ggtaaccttc gtcgccattt taaccgccgc gaatctgaag agcgtcaacc
    22261 tggtcgctaa cttcaatacc ctgtttgtac tggtgcaaat ctccatcatg gtggtgttta
    22321 tcttcctggt ggttcaggga ctgcataaag gagaaggcgt tggcaccgtc tggtcacttc
    22381 agccgtttat cagcgagaac gcgcacctga tcccgattat taccggtgcg acgattgtct
    22441 gtttctcgtt cctcggtttc gatgcggtga ccacgctttc ggaagagacg ccggacgccg
    22501 cacgcgtgat cccgaaagcc atcttcctga cggcggtata cggcggcgtt atctttatcg
    22561 cggcgtcgtt ctttatgcag ctgttctttc ccgatatcag ccgctttaaa gacccggacg
    22621 ccgcactgcc tgaaattgcg ctgtacgtcg gcggcaagct ctttcagtcg attttcctct
    22681 gcaccacgtt tgtgaacaca ttagcgtctg gcctggcttc acatgccagc gtgtcgcgtc
    22741 tgctgtatgt gatggggcgt gacaatgtgt ttccggagcg cgtgtttggc tatgtgcacc
    22801 caaaatggcg gactccggca ctcaacgtca ttatggtcgg gattgtcgcg ctgtcggcgc
    22861 tgttctttga tttagtcacc gcgacagcac tgattaactt cggtgctctg gtggcgttta
    22921 ccttcgtgaa tctgtcggtg tttaatcatt tctggcggcg taaaggaatg aataaaagct
    22981 ggaaggatca cttccactat ttgctgatgc cgctggttgg cgcgctgacg gtgggtgtgc
    23041 tgtgggttaa cctagagtca acgtcactga cactcggtct ggtatgggct tcgttaggcg
    23101 gcgcatattt gtggtatctg atccgccgct atcgcaaagt gccgctgtac gaaggtgaca
    23161 gaacgccggt gagcgaaacg taatagccgg atgcgcggcg cattatcctg cctgagggta
    23221 acgcgccgcg ccattaacta taaaaagatg tataacccgg atcacgagtg ctatcatccg
    23281 cccttatgct tagcgcatta accccagggg caaataatga accacgatat cccgctaaaa
    23341 tattttgata ttgccgatga gtacgcgacc gaatgcgcag agcctgtcgc agaggctgaa
    23401 cgcacaccgc tggcccacta cttccagtta ctgctcaccc gtttgatgaa caatgaagag
    23461 atcagcgaag aagcccaaca tgaaatggct gctgaagcgg ggattaatcc cgtacgcatt
    23521 gatgagatcg cagagttttt gaatcaatgg ggtaatgagt aattatttaa ttgcagacga
    23581 tcggtcagta tcgttgtccg gataaagcgt tcacgccgca tccggctaac aatgacacgc
    23641 actgcagcgt gctttaatgg gtttctcacg atcgtaccgc tctgctcctc gctaagttgt
    23701 tctgtgagtt atagttatag agaaatcacg ggtacgaatg ttgaacagat ggcgacgcca
    23761 cctgttcaac gaccaaccac acggccctca ttacgacccc tgaacacaaa cggtaatggc
    23821 aaatgtcatt aaatattagt aagatagcct ctttacataa aatcccttaa tattatgcgc
    23881 caggtattat cgtctctttt ggtgattgct ggacttgtga gtggtcaggc aatcgccgcg
    23941 cctgaatccc ccccgcatgc tgatatccgc gacagcggtt ttgtctattg cgtcagcggg
    24001 caagtcaaca cctttaaccc atccaaagcg agcagtgggt taattgtcga tacccttgcc
    24061 gcccagtttt atgatcgact gctggatgtc gatccctata cctatcgcct gatgccggaa
    24121 cttgccgaaa gctgggaagt actcgacaac ggcgcgacct atcgcttcca cctgcgtcgc
    24181 gatgttccgt ttcaaaaaac cgactggttt actcccactc gtaaaatgaa tgccgacgat
    24241 gtggtgttta ccttccagcg aatttttgac cgcaacaacc cgtggcataa cgtcaacggc
    24301 agcaacttcc cctacttcga cagcctgcaa tttgccgata acgtgaaaag cgtccgcaaa
    24361 ctggataatc ataccgttga gttccgactc gcccagccgg atgcttcttt tttgtggcac
    24421 ctcgcaaccc attatgcttc ggtcatgtcg gcagaatatg cccggaagtt agagaaagaa
    24481 gatcgccagg agcaactcga ccgtcaaccg gtcggcaccg gtccgtatca gttgtcggaa
    24541 taccgcgccg ggcaatttat tcgcctacaa cgtcatgatg acttctggcg cggtaaaccg
    24601 ttaatgccgc aggtagtggt ggatttaggc tccggcggca ccggacgtct gtcgaaactc
    24661 ctgaccgggg aatgcgacgt tctggcctgg cctgctgcca gccagctatc cattttgcgt
    24721 gacgacccgc gcttgcgttt aacgctgcgt cctgggatga acgtcgccta tctggcattt
    24781 aacaccgcca aaccgccgct aaataatccc gctgtccgcc atgcactggc actggcgatt
    24841 aataaccagc gcctgatgca atccatctat tatggtacgg ctgaaacggc ggcctctatt
    24901 ttaccgcgcg cctcgtgggc ctatgacaac gaggctaaaa ttactgaata caatccggcg
    24961 aaatcgcgcg aacagttgaa ggcgttgggg ctggaaaact taacgctgaa actgtgggtg
    25021 cccacacgtt cgcaggcgtg gaaccccagt ccactgaaaa ctgccgaact gattcaggcg
    25081 gatatggcgc aggttggcgt aaaagtggtg attgtgccgg tagaaggtcg ctttcaggag
    25141 gcgcggttga tggatatgag ccatgatctg acattatccg gttgggcgac ggacagtaac
    25201 gacccggaca gtttcttccg tcctttactg agctgcgcgg caattcattc acagaccaac
    25261 ctcgcccact ggtgcgatcc gaaattcgac agcgtgttgc gtaaggcgct ctcctcgcag
    25321 cagctggcgg cgcgtattga agcctatgac gaagcgcaga gtattctggc gcaggaatta
    25381 cctattctgc cgctggcgtc gtcattgcgt ttgcaggcct atcggtacga tatcaaaggt
    25441 ctggtactta gcccgtttgg taacgcctcc tttgctgggg tgtatcgcga gaaacaggat
    25501 gaggtgaaaa aaccatgatt atcttcacct tacgccgcat tttgttattg attgtcacct
    25561 tgttcctgct gaccttcgtt ggcttcagct tgagctattt cacccctcat gcgccgttac
    25621 aaggcgcgtc actgtggaat gcctgggtgt tctggtttaa cggcctgatc cactgggatt
    25681 ttggtgtttc cagcattaac ggtcaaccga ttgccgaaca gttaaaagag gtcttcccgg
    25741 cgacgatgga actgtgcatc ctcgccttcg gttttgcgct gattgttggc atccccgtgg
    25801 ggatgattgc cgggattacg cgccataagt ggcaggataa cttgatcaac gccatcgcct
    25861 tactgggctt ttcaatcccg gtgttctggc tggcgcttct gttgacgctg ttttgttcac
    25921 tcacgctagg ttggttgccc gtttcagggc gtttcgatct gctctacgaa gtgaaaccga
    25981 ttaccggttt tgcgttgatt gatgcctggc tttcggactc accatggcgg gatgaaatga
    26041 tcatgagcgc aatccgccat atgatattgc ccgtgatcac cctgtcggtt gcgccaacaa
    26101 ctgaagtgat ccgcctgatg cgtatcagca ctatcgaagt gtatgaccag aactatgtaa
    26161 aagcggcggc gacccgcggt ttgtcgcgct ttactatttt gcgtcgccac gttctgcata
    26221 acgcgttacc gccggttatt cctcgcctgg gcttacagtt ttccaccatg ctgacgctgg
    26281 cgatgatcac cgaaatggtt tttagctggc cgggtttagg gcgctggtta attaacgcca
    26341 tccgccagca ggattacgca gccatttccg ccggagtgat ggtgtgtggc tcactggtca
    26401 ttattgttaa cgtgatttct gatattttgg gtgccatggc taaccctctg aaacataagg
    26461 aatggtatgc cttacgatag cgtatacagc gaaaagcgcc cgccgggcac gctgcgtacc
    26521 gcctggcgca aattttatag tgacgcctct gcgatggtcg gcctgtacgg ctgcgcgggg
    26581 ctggctgtac tgtgtatttt tggcggctgg tttgcgcctt acggcatcga ccagcaattt
    26641 ctcggttatc aattattgcc gccatcatgg tcgcgctatg gcgaagtttc tttcttcctg
    26701 gggactgacg acctcggacg cgatgtttta agccgtttac tgagcggagc tgcgccaacc
    26761 gtgggtggcg catttgtggt gacgcttgcc gcgacgatct gtggcctggt actgggcacc
    26821 ttcgccgggg cgacacacgg cctccgctca gcggtgctta accatattct ggatacctta
    26881 ctggcgatcc cttcgctgct gttggcaatt atcgttgtag cgtttgccgg accgagtttg
    26941 tcccacgcta tgtttgctgt ctggctggca ttgctgccgc gtatggtacg ttcgatttac
    27001 agcatggtgc atgacgaact ggaaaaagag tacgttatcg ccgcccgtct ggatggcgca
    27061 tcaacgctga atattctctg gtttgctgtg atgccaaaca tcaccgccgg gctggtcacc
    27121 gagatcaccc gcgcactgtc gatggcaatt ctcgatatcg ccgcgctggg ctttctcgat
    27181 ctcggcgcac aactcccctc gcctgaatgg ggagcgatgc tcggtgatgc gctggaactg
    27241 atttatgtcg ccccgtggac tgtcatgctg ccaggtgcgg caattatgat tagcgtgttg
    27301 ttggttaacc tccttggcga tggcgttcgt cgtgcgatta ttgcgggggt ggaataatgc
    27361 cattactcga tattcgtaac ctgaccattg aatttaaaac cggtgatgag tgggttaaag
    27421 ccgtcgaccg ggtaagtatg accttaaccg aaggtgaaat ccgcggtctt gttggtgaat
    27481 ccggttccgg caagagtttg attgcgaaag cgatttgtgg ggtgaataaa gataactggc
    27541 gtgttactgc tgaccgtatg cgttttgatg atatcgattt gctgcgtctc tccgcacgcg
    27601 aacggcgcaa actggttggc cataacgtgt cgatgatttt ccaggaaccg cagtcgtgtc
    27661 ttgacccttc agaacgtgtg ggtcgccagt tgatgcaaaa catcccagcc tggacctata
    27721 aaggccgttg gtggcagcgt tttggctggc gcaaacgccg tgccattgaa ctgctgcacc
    27781 gcgtggggat taaagatcac aaagatgcga tgcgcagttt tccctatgag ttgaccgaag
    27841 gtgaatgtca gaaagtgatg atagccattg cactggcgaa tcaaccgcgt ctgctgattg
    27901 ctgacgaacc gaccaactca atggagccaa caacccaggc gcaaatcttt cgcctgctga
    27961 cgcgtctcaa ccaaaacagc aataccacta ttttgcttat cagccatgac ttacaaatgc
    28021 ttagccaatg ggcggataaa attaacgtgc tttactgcgg tcaaacagtg gaaaccgcgc
    28081 cgagtaagga gttggtgacg atgccacatc atccttatac ccaggcgctg atccgcgcga
    28141 taccagactt cggcagcgcg atgccgcata aaagtcgcct caatacgctg cccggcgcta
    28201 tcccactgct ggaacagtta ccgattggtt gtcgtctggg gccacgttgc ccgtatgcac
    28261 aacgagaatg cattgtgacg ccacgtttga cgggggcgaa aaatcatctc tatgcctgtc
    28321 atttcccgct gaacatggag aaagagtgag atgatcgaaa cgctgcttga agtgcgtaat
    28381 ttaagtaaaa cgttccgcta ccggaccggc tggtttcgtc gtcagaccgt agaagcggta
    28441 aaacccttga gctttacgct acgtgaaggc cagacactgg cgattattgg cgagaatggt
    28501 tcgggtaaat ccacgctggc aaaaatgctg gcgggaatga tagagcctac cagcggcgag
    28561 ttattgattg acgatcatcc actgcatttt ggcgattatt ccttccgcag tcagcgtatt
    28621 cgcatgattt ttcaggatcc ttcgacctcg ttgaatcccc gtcaacgtat ctcgcaaatt
    28681 ctggattttc cactgcgcct gaacaccgac ctggaaccgg aacagcgtcg caaacagatt
    28741 attgaaacga tgcgtatggt tgggctgcta ccggatcacg tcagttacta tccgcatatg
    28801 ttggcacccg gacaaaaaca gcgtctgggt ctggcgcgcg cgctaatatt gcgcccaaaa
    28861 gtcattattg ccgatgaagc cctcgcttca ctggatatgt cgatgcgttc gcagttgatt
    28921 aatctgatgc tggagttaca ggaaaaacag ggcatttcgt atatttatgt tacccagcat
    28981 atcggaatga tgaagcacat tagcgaccag gtgctggtga tgcatcaggg cgaggttgtc
    29041 gagcgaggca gcaccgcgga tgtgctggca tcgccgctgc atgaactcac caaacggctg
    29101 attgccgggc actttggcga agcattgaca gcggatgcgt ggcgtaaaga tcgctaattt
    29161 tctgaatcct ccttccagat tatccacctc attctgacaa gagattaaat cttgtcagaa
    29221 tgaggtgatg gataaaataa aatcaaatgc gcgtgattta agacgcaatc tcactttgca
    29281 ggaacgaaag ctctggcgat atcttcgcag ccgacgtttt ggtgatttca aatttcgccg
    29341 tcaacatccg gtggggagct acattctcga ttttgcttgc tgctcggcgc gcgtagtcgt
    29401 tgagctggat ggtgggcagc atgatttggc agttgcctat gataccaggc gcactagctg
    29461 gcttgagtcg cagggctgga ccgtgctgcg tttctggaat aacgagattg attgtaatga
    29521 ggaggcggtg ccggagatta ttctgcagga actgaaccgc cgatcaccct caccctgaaa
    29581 gggcgagggg gccgaccgag ccgaatagct gttgtggtga aaacatggag acagtgctgg
    29641 agaatattcg gcaaggtctg aaccatccca gccatcgcca taaaagggtt aggggctgta
    29701 tgagcctgtt tgttgctggg gtaacaatat ttgcacaata tggtcccctc gcccctctgg
    29761 ggagagggtt agggtaaggg gaaaagcgcc caccctgccg cagcctgctc cggtcggacc
    29821 tggcaactat agctactcac agccaggttg attataataa ccgtttatct gttcgtactg
    29881 tttactaaaa cgacgaatcg cctgattttc aggcacaaca agcatcaaca ataaggatta
    29941 aagctatggg ttttctttcc ggtaagcgca ttctggtaac cggtgttgcc agcaaactat
    30001 ccatcgccta cggtatcgct caggcgatgc accgcgaagg agctgaactg gcattcacct
    30061 accagaacga caaactgaaa ggccgcgtag aagaatttgc cgctcaattg ggttctgaca
    30121 tcgttctgca gtgcgatgtt gcagaagatg ccagcatcga caccatgttc gctgaactgg
    30181 ggaaagtttg gccgaaattt gacggtttcg tacactctat tggttttgca cctggcgatc
    30241 agctggatgg tgactatgtt aacgccgtta cccgtgaagg cttcaaaatt gcccacgaca
    30301 tcagctccta cagcttcgtt gcaatggcaa aagcttgccg ctccatgctg aatccgggtt
    30361 ctgccctgct gaccctttcc taccttggcg ctgagcgcgc tatcccgaac tacaacgtta
    30421 tgggtctggc aaaagcgtct ctggaagcga acgtgcgcta tatggcgaac gcgatgggtc
    30481 cggaaggtgt gcgtgttaac gccatctctg ctggtccgat ccgtactctg gcggcttccg
    30541 gtatcaaaga cttccgcaaa atgctggctc attgcgaagc cgttaccccg attcgccgta
    30601 ccgttactat tgaagatgtg ggtaactccg cggcattcct gtgctccgat ctctctgccg
    30661 gtatctccgg tgaagtggtc cacgttgacg gcggtttcag catcgctgca atgaacgaac
    30721 tcgaattgaa ataatcattc tgttggtaaa tattgggcgg cgttctgccg cccgttatct
    30781 ctgttatacc tttctgatat ttgttatcgc cgatccgtct ttctcccctt cccgccttgc
    30841 gtcaggataa cgatttcctt tacgaccaag gagcgcccat ggaacaacgc cacatcaccg
    30901 gcaaaagcca ctggtatcat gaaacgcaat ccagtactgc ggagtatgac gttctgcctc
    30961 tggtcccgga agccgcaaag gtcagcgatc cctttctgct cgacgtgatc cttgatgaag
    31021 aaacgctggc tcccttcctt tcatggctgg tccctgcgcg cgttcttgca gtggaattgt
    31081 tccctgacca gcttaccgtg acccgttcac agaccttcac cgcttatgaa cgcttgtcta
    31141 cggccctgac ggttgctcag gtttgcggcg tccagcggtt atgtaactac tattcggcgc
    31201 gacttacgcc gctccccggg cctgattcct ccagggaaag caatcatagg ttggcacaaa
    31261 tcacgcaata tgcccgccaa ctggctagct cgccttctat tatcgacaac cgatcgcgcc
    31321 agcatctgaa tgacgtcggt cttactgcct gggactgtgt gatcattaac caaatcattg
    31381 gttttattgg ctttcaggcg cggaccattg cgacatttca ggcttatctc gggcacccgg
    31441 tacgctggtt acccggtctg gaaatacaaa actacgccga cgcgtcactg tttgctgatg
    31501 aatcaatacg ctggcgaagc agctatgagg tggaaaaact acctgaagag tacacaaaaa
    31561 gttcaacagc agaactttgc caactggccg aaacactctc tctccaccct atttcacttt
    31621 cccttctcga aaggttgtta aacagtacac gggttaatac acagccggat aataagcttg
    31681 cggcgttgtt atgcgcgcgg ataaatggca gtcctgcttg ttttgccgcc tgtatggatt
    31741 catcaaatga atataaaaaa tcagccccct tctgcgcaag ggcgaaaatg aaattaacca
    31801 atgggctgac cgtcattctg ttgagcacgc taccgttcag gcgatacaat ggctgacccg
    31861 agcacccgat cgctttagcg ccgcccagtt cagcccttta ctcggacacg aaaaatcatc
    31921 aacgcagatt attaatctgc tggtatggag cgggctgtgt ggctggataa atcgcttaaa
    31981 aatcgcgttg ggtgagacat attaaccttg ccgcgtcaga cagattcgcg taaaactgtc
    32041 agccgctcta atggccacca aaatagacaa ttatgtttca ggacaacccg ctgctagcgc
    32101 agcttaaaca gcaactgcat tcccagacgc cacgcgctga aggggtggta aaagccacag
    32161 aaaaaggctt tggcttcctg gaagtcgacg cgaaaaaaag ttatttcatt ccgccgccgc
    32221 agatgaaaaa agtcatgcat ggcgaccgaa ttatcgcggt gatccacagt gaaaaagaac
    32281 gtgaatccgc agagccagaa gaactggttg aaccgttcct gactcgtttc gtgggtaaag
    32341 ttcagggcaa aaatgaccgt ctggccatcg ttcctgatca tccactctta aaagacgcca
    32401 ttccttgccg cgcagcccgt ggcctgaacc acgagtttaa agaaggcgac tgggcggttg
    32461 ccgaaatgcg ccgtcatccg ctgaaaggcg atcgttcttt ctatgctgaa ctgacacaat
    32521 acatcacttt tggtgacgac cactttgtac cgtggtgggt tacccttgcg cgccataatc
    32581 tggaaaaaga agcaccagac ggcgtcgcta ccgaaatgct cgatgaaggt ctggttcgtg
    32641 aagatctgac cgcgctggat tttgtcacca tcgacagtgc cagcacagaa gatatggatg
    32701 acgccctttt cgctaaggcg ttgccggatg acaaacttca gctgattgtg gcgattgccg
    32761 atccaaccgc gtggattgct gaaggcagca agctggacaa agccgcgaaa attcgcgcat
    32821 tcaccaacta tctgcctggc ttcaacatcc ctatgcttcc tcgcgagctt tctgacgatc
    32881 tctgctcact gcgcgccaat gaagtccgcc cggtactggc atgccgcatg acgctctccg
    32941 ctgatggcac cattgaagat aatatcgaat tctttgccgc caccatcgaa tccaaagcga
    33001 agctggtgta tgaccaggtt tctgactggc tggaaaatac cggtgactgg cagcctgaaa
    33061 gtgaagcaat tgccgaacaa gtccgtttgc tagcgcaaat ttgccaacgc cgcggcgagt
    33121 ggcgtcataa ccacgcactg gtgtttaaag atcgcccgga ttaccgcttt attctcggtg
    33181 aaaaaggtga agtgctggat atcgtcgccg agcctcgtcg cattgccaac cgtatcgtcg
    33241 aagaagcgat gattgccgct aacatttgtg cggcccgcgt tctgcgcgat aagctcggtt
    33301 ttggcatcta taacgtgcat atgggctttg atccggcgaa tgccgacgcg ctggcagcgt
    33361 tgctgaaaac gcacggtctg catgtcgatg ccgaagaagt gctcacgctg gacggtttct
    33421 gcaaactgcg tcgtgaactg gatgcgcaac caactggttt cctcgacagc cgcattcgtc
    33481 gcttccagtc atttgctgaa attagcactg aacccggtcc tcactttggc ctcggtctgg
    33541 aagcatacgc cacctggact tcgccgatcc gtaaatatgg cgacatgatc aaccaccgtc
    33601 tgctgaaagc ggttatcaaa ggcgaaactg cgacgcgtcc gcaggatgaa atcactgtcc
    33661 aaatggccga acgtcgccgt ctcaaccgga tggcagaacg tgatgttggc gactggttat
    33721 acgcacgctt cctgaaagat aaagccggga ccggcacccg tttcgcggcg gaaattgtcg
    33781 atatcagccg tggcggcatg cgtgtgcgtt tggttgataa cggcgctatc gcctttattc
    33841 cggcaccttt cttacacgct gtgcgcgatg aactggtttg cagccaggaa aacggcactg
    33901 tacaaattaa aggtgaaacg gtttacaaag taactgacgt tattgacgtc accattgccg
    33961 aagtccgcat ggaaacccgc ggcattattg cgcgcccggt cgcgtaatct cctttcacgg
    34021 cccattcctc atggatgggc cgtttatttc cccgctctac cttcgttata tccaggcaga
    34081 ttttttaact gactttcgtt tgaaaactgg cgtttttcca aaactggatt acaaataaat
    34141 atataaaaaa acaatgcgtt tagccgccgt taatttcatg taagtcatgt tgttcctttt
    34201 atttaaccgc ggactccgca gttaaccgga ggatatgcat catgaaaacc gttagggagt
    34261 ccacaacgtt gtacaacttt ctcggatcgc acaatccata ctggcggttg acggaaagca
    34321 gcgatgtttt gcgctttttt accaccgaaa ccacagaacc tgatcgtact ttgcagttat
    34381 ctgccgaaca ggctgctcgc atcagggaaa tgacggtcat cacctccagc ctgatgatga
    34441 gtctgaccgt cgatgagagc gatctttctg tgcatctggt aggacgaaaa atcaataaac
    34501 gggaatgggc tggcaacgcg tctgcatggc atgacacacc ggcagttgct cgtgatttat
    34561 cacacgggct ttcctttgct gagcaggtag tttctgaagc acattccgca atagtgattc
    34621 tcgacagccg ggggaatatc caacgcttca atcggttatg tgaagattac actgggttga
    34681 aagaacacga cgtcattggc caaagcgtgt ttaaactgtt tatgagccgt cgtgaagctg
    34741 cggcatccag gcgcaataac cgtgtatttt ttcgaagcgg caatgcatat gaagtcgaac
    34801 tgtggatacc aacacgtaaa ggccagcggc tgtttctgtt tcgcaataaa ataaatttgt
    34861 ccacagcggc agtggcaaaa acgagatttt tttaatctgt tccggtaccg acattaccga
    34921 agagcgccgc gctcaggagc gactgcgtat tctggcaaat accgacagta tcaccggact
    34981 gccgaatcgt aacgcaatgc aggagttaat cgatcacgct attaatcagg ctgataacaa
    35041 taaagttggg gttgtgtatc ttgatttgga taattttaaa aaggtcaacg acgcctatgg
    35101 gcatttgttt ggtgaccagt tattacgcga cgtgtcattg gctattttaa gctgtctcga
    35161 acatgaccag gtgttggcgc gtccaggtgg cgatgagttt ctggtactgg catccaatac
    35221 ctcacaaagc gcgctggaag caatggcatc acgaattttg acccgcttac gcctcccctt
    35281 tcgtattggt ttaattgaag tttataccag ctgttcagta ggtatttcgc tctctcccga
    35341 acatggttcg gacagcgcga ctattattcg tcacgccgac acagcaatgt acacagcgaa
    35401 ggaaggcgga cgaggacaat tttgcgtttt tactccagaa atgaatcagc gggtatttga
    35461 atatctctgg ctggatacca acttgcgtaa agcactggaa aacgatcagt tggttattca
    35521 ctatcaaccg aaaatcacct ggcgtggcga agtgcgcagt ctggaagcac tagtacgttg
    35581 gcagtcacct gaacgtgggt tgattccacc gttggacttc atttcctacg ccgaagagtc
    35641 agggctaatt gtgcctttag gccgttgggt gattctcgat gtcgtacgcc aggtggcaaa
    35701 gtggcgggat aaaggcataa acctgcgagt ggcggtaaat atttctgcac gtcagctcgc
    35761 cgatcaaacc attttcaccg ccctgaaaca ggttctccag gaactcaatt ttgaatactg
    35821 ccctatagat gttgaactga cagagagttg tctgattgag aatgatgaac tggcactgtc
    35881 tgttattcaa caatttagcc aactaggtgc gcaagtgcat ctggatgatt ttggtaccgg
    35941 ctactcttca ctttcgcaac tggcgcgctt tccgatcgat gccatcaaac ttgaccaggt
    36001 ttttgttcga gatattcaca aacaacctgt ctcgcagtca ctggtccggg cgatcgtcgc
    36061 tgtggcccag gcattgaatc ttcaggtgat cgccgaaggt gtagagagtg caaaggaaga
    36121 tgctttttta accaagaacg ggatcaatga gcggcaagga tttttgtttg ccaaaccgat
    36181 gcccgccgtc gccttcgaac gctggtataa acgctatctg aagcgcgcat aagtagatga
    36241 atatatatac ttagcgctat atcccgtcat tatatatcgt attcctggat gataattagc
    36301 cgttgctgtt aaacacttct cttttagcgg caatttttgc attattcatt ttccactttt
    36361 gctataagga tgtccttgat gtctgaattt gatgcccagc gcgttgccga acgtattgat
    36421 attgtgttag atatccttgt ggcaggcgat taccactctg cgatccataa tctcgagata
    36481 cttaaagcag aactcttgcg ccaggttgct gaatctacgc cagacatccc gaaggcaccg
    36541 tgggagattt aacaatcgtg ccttcaggtt ttcctatttg aggatgcccg gcgcgtcagt
    36601 atttttttat ttagtattat aacgttataa gaattacagc gatgaattcc cgacaacaaa
    36661 ctattctaca gatggtcatt gaccagggtc aggttagcgt aaccgatctg gcaaaagcca
    36721 ctggagtttc tgaagttacc attcgccagg atctcaacac cctcgaaaaa ctgagttacc
    36781 tccgccgtgc acatggcttt gcagtttcgc ttgatagtga tgacgtcgaa acccgtatga
    36841 tgagcaacta tacgctgaag cgtgaactcg ccgagtttgc cgcgtcactg gttcaaccgg
    36901 gcgaaaccat ctttatcgaa aatggcagca gcaatgccct gctcgcccgg actctggggg
    36961 agcagaagaa aaatgtcact atcatcacgg tcagcagcta catcgcgcat ttgctgaaag
    37021 acgcgccttg tgaagtcatt ttgctcggtg gcgtgtacca gaaaaaaagc gaaagtatgg
    37081 ttggcccgtt gacacgccag tgcatccaac aggtgcattt cagcaaagca tttattggta
    37141 ttgatggctg gcaacctgaa actggattca ccggtcgcga tatgatgcgt accgatgtgg
    37201 tcaatgccgt gctggaaaaa gagtgcgaag cgatagtcct gactgacagc tcgaaatttg
    37261 gtgctgtaca ttcatactcc atcggtcccg ttgagcgatt caatcgcgtg attaccgatt
    37321 cgaaaatacg cgccagcgat ctgatgcatc ttgagcacag taaactcacc gttcacgtcg
    37381 ttgacattta accatttccc ttctctacgg atgatttgca gtttggcaaa tcatccgctc
    37441 taagatgatt cctggttgat aattaagact atttacctgt tattaacacg cttaagatat
    37501 aaaattatta tcagcgatat aacaggaagt cattatcacc tgcgtgatat aaccctgcgc
    37561 gcgagcagat ttcacggaat aatttcacca ggcttattct tagctattat agttatagag
    37621 agcttacttc cgtgaatcat aaattcagga gagagtatta tgtttgtaac gagcaaaaaa
    37681 atgaccgcgg ctgttctggc aattactttg gcaatgtctc tgagtgcctg ttctaactgg
    37741 tctaaacggg accgcaacac cgcaatcggc gcgggtgcag gggcattagg cggtgcagta
    37801 ctgaccgatg gcagtacgtt gggtacatta ggtggtgcag ctgtcggtgg tgttattggt
    37861 caccaggtcg gtaaataatc gtaaataaat gtcgattatc gactcgcaat tataatacgc
    37921 ttgattctgt tcagtacgaa ataactgaat aatacgcaca gtaaaatatt cgaaagccac
    37981 ggatatatcc gtggcttttt atgttaaccg cctgcgagtt ttaccttcat ccctttcgct
    38041 tcaagtagtg attttaataa atcacgctta tcgccctgga tttcaataac tccatcttta
    38101 actgctccgc cgcagccgca ttttttcttc agttccgctg caagttttgt cagttcggca
    38161 tcatcgagat cgacaccggt aatcaggcaa acgcccttac ctttacgtcc actggtctga
    38221 cgctgaatac gcaccacacc gtcgccttta gggcgtacag gggccgcttt gggctcatca
    38281 atacgtccgg tttccgttga gtagaccaga cggctgttgg aatcactcat catgcactcc
    38341 gctgtaaaga ggcgttgatc gctttcagcg tctgcgctgg atctaccgat tgcgttaccg
    38401 ggcgaccaat caccatataa tcaacaccag ccgacaacgc ctgttctggc gtcataatgc
    38461 ggcgctggtc accagcttca ctcccctgcg gacgaatgcc cggcgtaacc agtttgaact
    38521 cctgaccgaa tacctgttta aagcgcacag cttcctgagc agaacacacc acaccatcaa
    38581 ggccacattt ttgcgtcagt gccgccagac gttctgcata atctgcaggt gacagtgtca
    38641 tgccaagatc gaccaggtcg ctggcttcca tgctggtcaa cactgtcaca gcaatcaaaa
    38701 gcggtgcatc tttgccaaac ggaaccagtg cctcacgcgc tgcggtcatc atacgcgccc
    38761 caccagaggc atgaacattc accatccaca cgcctaagtc agctgcagca gcgacagcgt
    38821 gcgctgcagt gttaggaata tcgtggaatt tcaggtcaag aaagatatca aaaccacgct
    38881 gttgaagttc gcgcacaaac tgtggcccaa acaatgtaaa catctctttg ccgaccttca
    38941 gacgacaatc gcgtgggtcg atcttgtcga caaaggacag cgcgtcatca cgattatgat
    39001 aatcaagggc aacaaccaca ggagaattcg taacagcgcg ggaagaagat gaagcagtta
    39061 acgtcatgac cagaccttct tgatgatggg caccggaagt ggcgcgaaac aggtaaacgg
    39121 cgagcattct acccgtggaa ataaaaaatt aacagttgcg atttttcccc tggcaggctt
    39181 tttctgctcc gtcgttgccg gagcacaatg gaacatcaat aactagtatg ttgtaactaa
    39241 agtcggattt ttttaaaaat tacaggccat caagaccgcg aatcggttta atggttgacc
    39301 aggcccgaca agacggacaa tgccagtaga gggtgtatgc ggtaaaacca catttctggc
    39361 agcgataacg aggcttacta cgtacctttt cgccaaccat gtcacgcagc accatcaggc
    39421 tctctttggc acgcccttct tccgcttcat ttaagtggta atccattaac ttatggaaca
    39481 cacgcatggt cggatgacgc tgaagctggc gcgtaatata gacctgtgcg gcctcactac
    39541 cgtcgcgcgc ttcgatgata tcagcaagca tcaattcagc atcggcaccg gtgttctctt
    39601 ccaccgcgcg ttgcaggaat tctgcccatt cggcagtttt acccaactgc tgatagcagg
    39661 tttgcaacat ttccagcgtt tcgctgacca gttctctgtc ctgggatatc acgcgttgca
    39721 aactttcgac ggctttggcg tattctcctt tcgccataaa cacgcgtccc atcatgatgg
    39781 atacgcgggc gctgttttta tctgccgccg cccctttttt tagcaaggtc atggcacgat
    39841 cgagatcgtc gctggccata tgctgcaggg ctaactcaca gtagaaatgg gcaatttcga
    39901 cgcgctgttt atctttaccc agcttcacca ggcgttcggc aacatcaatt gctttctgcc
    39961 actcgctggt agcctggtag atttgtagca actgttgcag cgcgccaatg cggaagtcag
    40021 tttcatcggt cagctgattg aacatgtctt ccgcgcggtc gtataacccg gcggccatgt
    40081 aatcacgccc cagttgttga atcgccaaca gacgctgttc ataggtcagt gaggcgcttt
    40141 ccattagggt ctgatggatg cgaatagcgc gatcaacttc gccacgcgaa cggaacaggt
    40201 ttccgagcgt aaggtgggct tcaacggtgc ctgtatcctc tttaagcata tcgagaaaca
    40261 ggtctaccgc tttatcctgt tgattactaa gcaggaagtt aacccccgct acgtaatcac
    40321 gcgacaagcg gttggcttca tcttgcttgt tttgttgcgc acttctgcgg cccatatacc
    40381 agccataggc agcggctaca ggcaaaagca gaaacaacaa ctccagcata gaaagttatt
    40441 ccttcgccgc tgacgagtgc ggcactacag ccacgtcagt cgcgggtgaa agctggtttt
    40501 ccagtcgctt tattttacgt tcagcgcgcg ccagggaaac acgaactcgc agccagaaca
    40561 ggccacaaat caaccaaccg atagcaaccc ccgcagcaaa caataccgcc agcaatgtgg
    40621 aaatacggta ctccccttgc gctaacagat aattaaacgt cacctgttga tcgttctgcg
    40681 cacccaacgt gaccgaaatc acgaagatcg ctaacaccag taagaaaatg agtaaatatt
    40741 tcacattact tcccgttatg tggttaaagc gaataaatta tggtcaactt accgcaatag
    40801 ccctttaaag taccattttc gcgccgtctg cgaaatggca tccacgcgca ttgttgatga
    40861 tttaagggct aaattcggaa aatcaaccag cgttaacttt cttgttctcg ttgcgctatt
    40921 tcgcgatttt cttccgcagg tggtgttaat ggcccacaaa ttcgttgcgc aagccacgtt
    40981 gccaccgcca ccagcgccca cgaaatcaac gtagctacta ccagatcgcg tggccaatgc
    41041 atcccgagca gcaggcggct tcccatgact cccgttgccc agaccagcaa gatagcaatg
    41101 gttaacgttc gccgacgcgg ccacagcaaa ccaacggcca gcagtgccca actggcagca
    41161 aacatcgtgt gaccggaagg aaaggcaaac cccgtctctt tctgccagtg tgaacgcaaa
    41221 tattgtggga tatttttctc ttcagccaac tgttctttca ctagatttcc gcgttctgct
    41281 cgctttaaag tgtagaactc atcaaccgga atatgatgtg ttttttccag ccagataaca
    41341 aaaggtcgtg gttcctggac tttgtctttg atccaggatt taacgccttg tcccacaatg
    41401 attacggccg ccagaatggc aaataataca aaggcagcct taatgcaaaa acgcagacac
    41461 cagagaaacc agccgaataa aatcaaatgt gtaatgacgc cccagggctg ggtgacagtt
    41521 tcagtaaccc aaaaagccgc ttttagtagc caactttgtt ctccaggttg ccaacgccag
    41581 ccagaaatcc atacggctac tggcatgaca agcaatagtg cagctcccac tgcggtacgt
    41641 ctggcaatcg aacgcatggc ctctcctttt tgataagtcc cacaatcata actgaaaacg
    41701 ccagttccag gaaaaattga cagatttgtg ccattccgtg aacgatcgac gcgtcgtgat
    41761 taggtgaacc ccttctcgtt atggcaaaat aagccaatac agaaccagca ttatctggag
    41821 aatttcatgc agcttaaacg tgtggcagaa gccaaactgc caaccccatg gggcgatttc
    41881 ctgatggtgg gatttgaaga actggcaacc ggacacgatc atgtcgcgct agtctatggc
    41941 gatatttccg ggcatacccc ggtacttgcg cgcgtccatt ccgaatgtct gaccggtgac
    42001 gccctgttca gcttgcgctg cgattgtggc ttccagctcg aagcggcatt gacgcaaatt
    42061 gccgaggaag gccgtggtat tttgctgtat caccgtcagg aaggtcgtaa cattggtctg
    42121 ctgaataaaa tccgcgctta cgcactgcag gatcaaggtt acgataccgt agaggctaac
    42181 catcagttag gcttcgccgc tgatgagcgc gacttcactc tttgcgctga tatgttcaaa
    42241 ctccttggcg tcaatgaagt ccgcttgtta accaataacc cgaaaaaagt cgaaattctg
    42301 accgaagcag ggattaatat tgttgaacgc gtaccattga ttgtaggtcg taaccccaat
    42361 aacgaacatt atctcgatac caaagccgag aaaatgggtc atttgctgaa caaataaccc
    42421 tctcgcattg tggaattaat ttgcttgccg gaagcaaaat aaccggcaag caaatatttg
    42481 ttacttcagc atattacgaa tgacataatg caaaatgccg tcgttctggt agtaggtcaa
    42541 ctccgtcgcg gtgtcgatac gacaacggca gggtacgacc tcctggctac catccgcgcg
    42601 cgtaagcgtc accggaaccg tcgcaccggg ttgtagattt tgcagatcgc caatatcaat
    42661 cttctcttcc ccggttagtc ctaacgtttt acgcgttacg ccttgcggaa attccagcgg
    42721 caggatgccc atgccaatta aattcgaacg gtgaattcgt tcaaacgatt cggcaatcac
    42781 cacacgaatt ccaagcagac gaggaccttt tgccgcccag tcacggcttg agcctgaacc
    42841 atactctttc ccggcaatca ccgccagcgg cgtttgttcc tgcttatagc gcatcgcagc
    42901 atcataaata gagactacgt cgctgtcagg taaatgccgc gtcatccccc cttcaacgcc
    42961 aggcaccatt tcattacgga tgcgaatatt ggcgaaggtg ccgcgcatca tcacttcatg
    43021 gttaccacgc cgcgaaccgt aggagttaaa gtcttttcgc tcaacacccc gaccttgtag
    43081 atatcgaccc gctgggctgt cgggcttaat actgcccgcc ggagagatat ggtcagtggt
    43141 gactgaatcc cccagcattg cgaggatccg cgcaccgtga atatcttcca ctggtgctgg
    43201 tgttgcctgc atttcatcaa agaaaggcga taagcgaata taggttgagt cctcctgcca
    43261 accgtaggta tcggatcgtg tgacgttaat ttccttccac tctgctgtgc cttcaaaaac
    43321 ttctgcgtac tctttgcgga acatttctgt ggagacttgt tctaccgcac gggcaatttc
    43381 ttgtgccgat ggccagatat ctttcagata aaccggatcg cctttacgat catgaccaat
    43441 aggctcagaa gccaggttga tattcatatt tcccgccagc gcataggcaa ccaccagcgg
    43501 cggcgaggcc agccagttgg ttttaaccag cggatggata cggccttcaa agttacggtt
    43561 gccggacagc actgcaccga cggttaaatc gccttttttg attgccgttt cgataggatc
    43621 gggcagcggc ccagagttac caatacaggt agtacaaccg tatcccacaa ggttaaaccc
    43681 cagttcgtcg agaaacggtg tcagtttcgc ttttgccaga tagtcagaaa caactttcga
    43741 gcctggtgcc agcgacgctt tgacccacgg ttgccgcttg aggccaagaa ttacggcttt
    43801 tttcgccagc aagcctgcag ccatcagcac acttgggtta gaggtgttgg tgcacgaggt
    43861 tatcgcagca atgaccacag cgccatcagg taactgatac tgatgtccgt tcataacata
    43921 atcgaccggc tggcgatctt tatgcgtggc attcacttcc agttcgttac tggcggcaaa
    43981 tacttttggt acatcgggca gtgcaacgcg atcctgtggg cgttttggcc ctgccaggct
    44041 cgcttcaacg tcattcatat ccagttctaa cgtactggta aaaatcggtt catcgcccgg
    44101 gttacgccac atgccctgcg ctttggcata tttttcgacc aactcaacct gatcttcgct
    44161 gcgcccgctt aaacgcatgt aatcgagggt tacagcatcg attgggaaga agccacaggt
    44221 ggcaccatat tctggcgaca tattggcaat ggtggcgcga tccgccagcg gtagtgaatc
    44281 cagaccatca ccataaaatt cgacgaattt ctccaccacg ccatgtttgc gcagcatttg
    44341 ggtaacagtg agaaccaggt ctgtggcggt aataccttca cgtaatttac cggtaagttt
    44401 aaagcccact acgtccggga taagcatgga aaccggctgg cctaacattg cggcttctgc
    44461 ttcgatccca cccacgcccc accccagcac gccaaggccg ttgatcatag tggtgtgcga
    44521 gtcagtacca acgagtgtat ccggataagc aatccattca ccgtcctgca attcactcca
    44581 cactgctttg ccgagatatt cgaggttaac ctgatggcaa atgcctgtgc ctggcggcac
    44641 gacgctaaac cgactgaacg cttgctttcc ccatttcagg aacacataac gttcgtggtt
    44701 gcgctccatt tccaggcgta cgttttcttc aaatgcctca tcatcaccaa aacgatcgac
    44761 ggtcaccgag tggtcaatga ccaggtcgac cggtgagagc gggttaacct ttgcagtatc
    44821 gccgccgagg cgtttaaccg cttcgcgcat tgccgccaga tcaacaacgg caggtacgcc
    44881 ggtaaagtcc tgcatcaaca cccttgccgg gcggtaggca atttcacggt cagcatgggc
    44941 atttttcagc catcctgcca gcgcgtggat atcctcttcg gtaaccgagt taccatcctg
    45001 ccagcgcagc aggttttcga gcaaaacttt gagtgacttg ggtagacggg tgatatcgcc
    45061 cagtgattta gcagcaagcg gcaggctgta gtagtggtaa gttttatctt tggcctgcaa
    45121 cgtgtcctta ctggcttctc gtagggttga cgacatagct cctccttaat gacagggttg
    45181 cgaagccctg attctcttca gggtgaatat taaagataac acaaacatcg cgtaacgatt
    45241 tgataacaac ccaaattgat aaaagaggag aaaccttgtg gcaaagcaaa tgacaacccc
    45301 gccgcagcgg ggtcaaggat cagtgagtaa tgatccagag gagttcggcc cagaagagca
    45361 tcgacagaga aaacgcgccc atccaggacc aatatttcat atttgtgttg atcataggct
    45421 acacccttaa cggttattat tttgtgttga gataactcaa gctgttattg ctgcggtgta
    45481 ttaaaagccc tttaattcat taaaaatggg taataagaat gatgataatt attgtttcgg
    45541 ctgatcatct tcagcaaaca gttctataaa ggcttgctgc tgcgatgtta gttgccagtc
    45601 gtctggaata acgacggaac attcccgttt cgtttctgtt tcattgtcac tatgctggca
    45661 taatggtgac gactccagtt gctcctgacc gaccatagtt atccccacac tttccaaatc
    45721 agcagtgcga caaccaccca gaaaagtgca gaaccaagaa aaaccgccag ccaggcttta
    45781 cgttttaatt caggatcgcg gcggggttcc tgatttccag aaggcattgc taacctcata
    45841 taatcgccat cacttatcag caagacgttg aacgattggt gtaattaatc atacgatgag
    45901 acaaatccta aagaaacttt agt
//
LOCUS       DAZPAC010000007        43715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZPAC010000007 DAZPAC010000000
VERSION     DAZPAC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.007"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..559)
                     /locus_tag="ACHUPM_000320"
     CDS             complement(<1..559)
                     /locus_tag="ACHUPM_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR0868211.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHUPM_000321"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHUPM_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR0868212.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHUPM_000322"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHUPM_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR0868213.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHUPM_000323"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHUPM_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR0868214.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="ACHUPM_000324"
     CDS             complement(4001..6322)
                     /locus_tag="ACHUPM_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR0868215.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHUPM_000325"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHUPM_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR0868216.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="ACHUPM_000326"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="ACHUPM_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR0868217.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="ACHUPM_000327"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="ACHUPM_000327"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR0868218.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHUPM_000328"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHUPM_000328"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR0868219.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..12968
                     /locus_tag="ACHUPM_000329"
                     /pseudo
     CDS             11268..12968
                     /locus_tag="ACHUPM_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHUPM_000330"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHUPM_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR0868220.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHUPM_000331"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHUPM_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR0868221.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHUPM_000332"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHUPM_000332"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR0868222.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHUPM_000333"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHUPM_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR0868223.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="ACHUPM_000334"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="ACHUPM_000334"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR0868224.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHUPM_000335"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHUPM_000335"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR0868225.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="ACHUPM_000336"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="ACHUPM_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR0868226.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="ACHUPM_000337"
                     /pseudo
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="ACHUPM_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="ACHUPM_000338"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="ACHUPM_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR0868227.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="ACHUPM_000339"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="ACHUPM_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFR0868228.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHUPM_000340"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHUPM_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR0868229.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="ACHUPM_000341"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="ACHUPM_000341"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR0868230.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="ACHUPM_000342"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="ACHUPM_000342"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR0868231.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="ACHUPM_000343"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="ACHUPM_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR0868232.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="ACHUPM_000344"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="ACHUPM_000344"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0868233.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="ACHUPM_000345"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="ACHUPM_000345"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR0868234.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="ACHUPM_000346"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="ACHUPM_000346"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR0868235.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="ACHUPM_000347"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="ACHUPM_000347"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR0868236.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31280..32185
                     /gene="yhfZ"
                     /locus_tag="ACHUPM_000348"
     CDS             31280..32185
                     /gene="yhfZ"
                     /locus_tag="ACHUPM_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR0868237.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32202..32564
                     /locus_tag="ACHUPM_000349"
     CDS             32202..32564
                     /locus_tag="ACHUPM_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR0868238.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32648..33811
                     /locus_tag="ACHUPM_000350"
                     /pseudo
     CDS             32648..33811
                     /locus_tag="ACHUPM_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33811..35037
                     /locus_tag="ACHUPM_000351"
                     /pseudo
     CDS             33811..35037
                     /locus_tag="ACHUPM_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35034..35912
                     /locus_tag="ACHUPM_000352"
     CDS             35034..35912
                     /locus_tag="ACHUPM_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR0868239.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35923..36276
                     /locus_tag="ACHUPM_000353"
     CDS             35923..36276
                     /locus_tag="ACHUPM_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR0868240.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36288..37592
                     /locus_tag="ACHUPM_000354"
     CDS             36288..37592
                     /locus_tag="ACHUPM_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR0868241.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37604..38689
                     /locus_tag="ACHUPM_000355"
     CDS             37604..38689
                     /locus_tag="ACHUPM_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR0868242.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38841..39572)
                     /gene="frlR"
                     /locus_tag="ACHUPM_000356"
                     /gene_synonym="yhfR"
     CDS             complement(38841..39572)
                     /gene="frlR"
                     /locus_tag="ACHUPM_000356"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR0868243.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="ACHUPM_000357"
     CDS             complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="ACHUPM_000357"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR0868244.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="ACHUPM_000358"
                     /pseudo
     CDS             complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="ACHUPM_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="ACHUPM_000359"
     CDS             complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="ACHUPM_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR0868245.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTEC
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42378..43715)
                     /gene="frlA"
                     /locus_tag="ACHUPM_000360"
     CDS             complement(42378..43715)
                     /gene="frlA"
                     /locus_tag="ACHUPM_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFR0868246.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGELIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT        10158 a        11126 c        11988 g        10443 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctggttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac gattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggtagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctagctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattc
    31261 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
    31321 gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
    31381 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgttgga
    31441 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat
    31501 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
    31561 gccctatacc cgcttgtacg aaggtttagc gagcgggctg aaagctcagt ttgatggcat
    31621 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
    31681 cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca cgcaaaaagg
    31741 gttatgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc agttgatttg
    31801 ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt cggcggatca
    31861 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg atctctctta
    31921 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt
    31981 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag acgatccgcg
    32041 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
    32101 actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac gggtagtgag
    32161 tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc agactcaacc
    32221 tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg caggtcgtca
    32281 acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga
    32341 cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata
    32401 acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg caattgcatc
    32461 aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc tatttgctgg
    32521 ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc
    32581 acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg
    32641 caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt ccgccgcact
    32701 aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg tggatcaggt
    32761 actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg aactgtatct
    32821 gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg cattaggtta
    32881 cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc
    32941 ggtggcacat caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga
    33001 acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc
    33061 agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg acgatgattt
    33121 tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg tggttgctga
    33181 aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt gcctgctttg
    33241 ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga tacaggcacg
    33301 ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt cagcgaccag
    33361 ctgcacttcg ctgccattac tggcgtaata cggtgtgact catgccgaac ccggtcatgc
    33421 gctgacgggc actattccgg caaaccagca gggcgatcag tctgaacgta tcgcgatgct
    33481 ctggttgagt gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta
    33541 ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac
    33601 tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgt cgctggcagg
    33661 cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc acgcagattt ttgtcacccg
    33721 tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccgaaaatcg ttggtcgtta
    33781 tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt agtgattgat
    33841 agctttggcg taggggcaat gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg
    33901 aatacatgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagaag
    33961 ctggggctaa tcaacgcatt gggttatgcg ccaggcgata tgcagccgtc agattccgca
    34021 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    34081 attttaggca cgcgcccgtt accgccgctg cggatgcctt ttcgcgatgt gattgaccgt
    34141 gttgagcagg cattagtatc cgccggttgg caggtggagc gccgtggcga tgaactgtaa
    34201 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa
    34261 gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat caaaattggt
    34321 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    34381 gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    34441 gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc
    34501 gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg
    34561 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    34621 agccagcgga ttatggatat caccctcgac gaatttaaca cccatccgac ggcgtttatt
    34681 tgcaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    34741 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgatgcgat gcagccagat
    34801 gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca cagccaccat
    34861 acccgcgaag tggtgccgct gctggtttat cagcaaggcc tggctgctac gcagctcggt
    34921 gtgcgcacca cgctttctga tgtgggagct accgtgtgtg aatttttccg tgcgccaccg
    34981 ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac cctatgagtt
    35041 ttgatccgac gggttacacc ctggcccatg agcatctgca tattgatctc tccggcttta
    35101 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    35161 tgatgacccg gggtgtacgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    35221 cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt
    35281 attaccagga tgcgtttttc ccggaacatg tggtgacccg cagcgtgcag gaactggcgc
    35341 aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa gccgggatca
    35401 tcgcggagat cggctccagc gaaggaaaga ttacgccgct ggaagagaag gtatttattg
    35461 ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    35521 ccatggggct ggaacaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    35581 ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg atcgatctcg
    35641 gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac gaaaagcgca
    35701 ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    35761 atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac tatttattga
    35821 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    35881 tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    35941 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctac tggcagtttt
    36001 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg gcaactggat
    36061 tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg
    36121 attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa agacgagcat
    36181 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa
    36241 cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg gatctgtata
    36301 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    36361 cggctgtttt tcatgacggt atccgcccga tcctgccgca actgattgaa ggctatatga
    36421 accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    36481 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    36541 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggc gctatctggg
    36601 gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg ctgccggtgg
    36661 atgtattagg tagcctcggg gaattaagct cgccggtggt ttctgctttt gcactgttcc
    36721 cgctggtggc gattttctac cagtttggct ggaagcaaag tctggtcgcc gccgtggttg
    36781 ttctgatgac ccgtgtggta gtcgtgcgct atttcccaca tcttaaccct gaatccatcg
    36841 aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc
    36901 gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga
    36961 ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc gttgccagca
    37021 tgaagatttt cgccggcagt gaagtgtcga tcttcactct ggagaaagcg tactccgcag
    37081 gcgtaacgcc ggaacaatcg caaacgctga tcaaccaagc ggcactggca gaatttatgc
    37141 gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag
    37201 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atagttgcag
    37261 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttcg atcggcaaat
    37321 ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa catccgtaac gccatgaata
    37381 tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag atggcgggtt
    37441 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    37501 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg ctgaatgttc
    37561 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    37621 gcaaagtctg acgattattg aggcgcagca aaagcagttt gcgctggtgg atagcatttg
    37681 tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa cgccaggact
    37741 gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca
    37801 ggctgcggcg ctggtgcagg gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt
    37861 gctcaaaccg gggcagcgtc ttctggtaca tgatgcgcct gtttacccga cgacacaggt
    37921 cattattgag cagatggggc tgacgcttat tactgctgat ttcaatgacc tgtcggcact
    37981 gaagcaggtc gtcgacgagc aactaccgga tgcggcgctg gtgcagcata cgcgccagca
    38041 gccgcaggac ggctacattc tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc
    38101 tgcattaacc gatgacaact atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg
    38161 ggcaaatgtc tcgacttttt cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt
    38221 ggtaggcgat gccgatgtta ttagtcggat tcgctccacg ctctattccg gcggcagcca
    38281 gattcagggc gctcaggcgc tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca
    38341 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc
    38401 ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca
    38461 tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc
    38521 ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt ccggaacgtt
    38581 tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga atcgcaacgg
    38641 tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat gctggatagc
    38701 accgtcaagc taaattccgt actgaacggt cccctcgccc ctttggggag agggttaggg
    38761 tgaggggaac gtacgggatg taaaattcaa cgcgccagca tcgccggatg cgatgctggc
    38821 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    38881 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    38941 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39001 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39061 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39121 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39181 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39241 acccgttcct gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg
    39301 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39361 ttgccctgcc agcggatcag tacgccgtct gccactaagt cgctgatagc tttgcgaata
    39421 gtaatgcggc tgacgttata ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg
    39481 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    39541 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    39601 cacggattat agggtcgttt tccggcaagt aacagcacgt tggcgcggag atgctcacgc
    39661 caacgttata cctaccaggc accgtgatac tgaatggttt tcgccgcgca cgccgttccc
    39721 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    39781 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    39841 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    39901 gcgccacggg caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg
    39961 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40021 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40081 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40141 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40201 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40261 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40321 aaggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40381 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40441 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    40501 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac
    40561 cctcatagcc ccgctcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc
    40621 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgataat
    40681 ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata cggcgcgcaa
    40741 atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca
    40801 ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat tagatccatg
    40861 ccgatgtttt ccgcgtactc acacagttcg cttaggttct ctgccagccg tccccagata
    40921 acattaggtg gcgtgagata gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc
    40981 atctcttttg ccatatccat cgccagcttg atcatgtcga ggctttcgcg acgcatatgt
    41041 tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtata gccgataatc
    41101 ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc ttttaagtcc
    41161 ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc cagctcgctt
    41221 gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa catacctgtt
    41281 ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga gggattaata
    41341 ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt aaatagacag
    41401 gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc acgggtgcag
    41461 cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct gtttaacaaa
    41521 gttaatggcg cattcggtgg tgtggcgact ttcatcattg ccgagcagga acaggaacgg
    41581 aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct caatcacgca
    41641 gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac ccagcggacg
    41701 cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt caccaagctg
    41761 gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca acggcttgag
    41821 atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca tctccagcac
    41881 cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat aatcaatgct
    41941 aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc
    42001 gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac cgtagtcaga
    42061 aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc agaactccca
    42121 gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg
    42181 cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac
    42241 catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt tttccacttc
    42301 ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat ccaacatttt
    42361 attttctcca gataacatta cgacaaagcg ttgagctggc ggctacgctt cgcccagaag
    42421 gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc gggaatcggt
    42481 gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag ggtggtcatc
    42541 agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg
    42601 atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata acccagcagg
    42661 ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag gatgatggag
    42721 acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa caggttgtct
    42781 ttcgccatcg cgtattccag ccgcggctgg tacatcacgc agctggaaag cgaaccaaga
    42841 atgacgatca tcgccgtgat ggcaacaaag atcccagcgg tgctgccgag tgcggggatc
    42901 caggtcaggg cgtcggaaat cggcgtttca gaattggcga gtttgtcgaa gggcatcagg
    42961 ccggaaatca ccagcgccag cagggtgtag agcaccagaa ccagcagaca ggaaccaatc
    43021 agcgctcgtg gcatggtttt tccggggttt ttaatttcgc cggtcatata acagatagag
    43081 gccatgccgg tatacgacca actggtggca gagatccccg ccagcagcgc cataaagctg
    43141 cccgttgcgc caatcgcagt ggtggtaggg gcggcaaaat tctccgcttt aaaccagaag
    43201 atcccaaggc caatgacgat agtgaacggg ataattttgg cgatggtaat tagcgtctga
    43261 aacgctgcgc cgccttcaac ggagcgcagg tgtagcaaca taaaggcgat aattaatccg
    43321 gcggcgataa atttaccgag caacggatcg ataggcgtta aaaagccaag attgctgaca
    43381 atcgccagcg ccataatcga caatgacggc gcatcgttgg cccagaagct ggcccagccg
    43441 gagaggaaag ccagcggtcg gcttccggca tttttcagat aaacataatc tgcgccattt
    43501 tccggataag cggtggatag ttccgcatag acgcacattt gcgggatcac aattaactcg
    43561 ccaatgacaa acgcgagcac cgtaagccac ggcgtgcccg ctgcttttgc cacttcaccc
    43621 acagatacaa aaataccgga gccgacggtt gtcccgacgg cgattgcaag aacggcccaa
    43681 aatccgagct tgcgttggag ttcctggctt cccat
//
LOCUS       DAZPAC010000008        43228 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZPAC010000008 DAZPAC010000000
VERSION     DAZPAC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43228
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.008"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            12..530
                     /locus_tag="ACHUPM_000361"
     CDS             12..530
                     /locus_tag="ACHUPM_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0868247.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            546..1085
                     /locus_tag="ACHUPM_000362"
     CDS             546..1085
                     /locus_tag="ACHUPM_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR0868248.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1180..2757)
                     /gene="guaA"
                     /locus_tag="ACHUPM_000363"
     CDS             complement(1180..2757)
                     /gene="guaA"
                     /locus_tag="ACHUPM_000363"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR0868249.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2826..4292)
                     /gene="guaB"
                     /locus_tag="ACHUPM_000364"
     CDS             complement(2826..4292)
                     /gene="guaB"
                     /locus_tag="ACHUPM_000364"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR0868250.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4454..5824
                     /gene="xseA"
                     /locus_tag="ACHUPM_000365"
     CDS             4454..5824
                     /gene="xseA"
                     /locus_tag="ACHUPM_000365"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR0868251.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5821..6036)
                     /locus_tag="ACHUPM_000366"
     CDS             complement(5821..6036)
                     /locus_tag="ACHUPM_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR0868252.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6105..7577)
                     /gene="der"
                     /locus_tag="ACHUPM_000367"
     CDS             complement(6105..7577)
                     /gene="der"
                     /locus_tag="ACHUPM_000367"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR0868253.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7695..8873)
                     /gene="bamB"
                     /locus_tag="ACHUPM_000368"
     CDS             complement(7695..8873)
                     /gene="bamB"
                     /locus_tag="ACHUPM_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR0868254.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8884..9504)
                     /gene="yfgM"
                     /locus_tag="ACHUPM_000369"
     CDS             complement(8884..9504)
                     /gene="yfgM"
                     /locus_tag="ACHUPM_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR0868255.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9522..10796)
                     /gene="hisS"
                     /locus_tag="ACHUPM_000370"
     CDS             complement(9522..10796)
                     /gene="hisS"
                     /locus_tag="ACHUPM_000370"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR0868256.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10907..12025)
                     /gene="ispG"
                     /locus_tag="ACHUPM_000371"
                     /gene_synonym="gcpE"
     CDS             complement(10907..12025)
                     /gene="ispG"
                     /locus_tag="ACHUPM_000371"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR0868257.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12052..13065)
                     /gene="rodZ"
                     /locus_tag="ACHUPM_000372"
     CDS             complement(12052..13065)
                     /gene="rodZ"
                     /locus_tag="ACHUPM_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR0868258.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13350..14504)
                     /locus_tag="ACHUPM_000373"
     CDS             complement(13350..14504)
                     /locus_tag="ACHUPM_000373"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR0868259.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14654..15085)
                     /gene="ndk"
                     /locus_tag="ACHUPM_000374"
     CDS             complement(14654..15085)
                     /gene="ndk"
                     /locus_tag="ACHUPM_000374"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR0868260.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15234..17546)
                     /gene="pbpC"
                     /locus_tag="ACHUPM_000375"
     CDS             complement(15234..17546)
                     /gene="pbpC"
                     /locus_tag="ACHUPM_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR0868261.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17547..22508)
                     /gene="yfhM"
                     /locus_tag="ACHUPM_000376"
     CDS             complement(17547..22508)
                     /gene="yfhM"
                     /locus_tag="ACHUPM_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFR0868262.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNCLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22715..23560
                     /gene="sseA"
                     /locus_tag="ACHUPM_000377"
     CDS             22715..23560
                     /gene="sseA"
                     /locus_tag="ACHUPM_000377"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR0868263.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23896..24021
                     /gene="timP"
                     /locus_tag="ACHUPM_000378"
     CDS             23896..24021
                     /gene="timP"
                     /locus_tag="ACHUPM_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR0868264.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24053..24829)
                     /gene="sseB"
                     /locus_tag="ACHUPM_000379"
     CDS             complement(24053..24829)
                     /gene="sseB"
                     /locus_tag="ACHUPM_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR0868265.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24971..26254)
                     /gene="pepB"
                     /locus_tag="ACHUPM_000380"
     CDS             complement(24971..26254)
                     /gene="pepB"
                     /locus_tag="ACHUPM_000380"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR0868266.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26432..26632)
                     /gene="iscX"
                     /locus_tag="ACHUPM_000381"
     CDS             complement(26432..26632)
                     /gene="iscX"
                     /locus_tag="ACHUPM_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR0868267.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26644..26979)
                     /gene="fdx"
                     /locus_tag="ACHUPM_000382"
     CDS             complement(26644..26979)
                     /gene="fdx"
                     /locus_tag="ACHUPM_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR0868268.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26981..28831)
                     /gene="hscA"
                     /locus_tag="ACHUPM_000383"
     CDS             complement(26981..28831)
                     /gene="hscA"
                     /locus_tag="ACHUPM_000383"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR0868269.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28848..29363)
                     /gene="hscB"
                     /locus_tag="ACHUPM_000384"
     CDS             complement(28848..29363)
                     /gene="hscB"
                     /locus_tag="ACHUPM_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR0868270.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29459..29782)
                     /gene="iscA"
                     /locus_tag="ACHUPM_000385"
     CDS             complement(29459..29782)
                     /gene="iscA"
                     /locus_tag="ACHUPM_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR0868271.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29799..30185)
                     /gene="iscU"
                     /locus_tag="ACHUPM_000386"
     CDS             complement(29799..30185)
                     /gene="iscU"
                     /locus_tag="ACHUPM_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR0868272.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30213..31427)
                     /gene="iscS"
                     /locus_tag="ACHUPM_000387"
     CDS             complement(30213..31427)
                     /gene="iscS"
                     /locus_tag="ACHUPM_000387"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR0868273.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31668..32156)
                     /gene="iscR"
                     /locus_tag="ACHUPM_000388"
     CDS             complement(31668..32156)
                     /gene="iscR"
                     /locus_tag="ACHUPM_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR0868274.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32457..33194)
                     /gene="trmJ"
                     /locus_tag="ACHUPM_000389"
     CDS             complement(32457..33194)
                     /gene="trmJ"
                     /locus_tag="ACHUPM_000389"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR0868275.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33313..34116
                     /gene="suhB"
                     /locus_tag="ACHUPM_000390"
     CDS             33313..34116
                     /gene="suhB"
                     /locus_tag="ACHUPM_000390"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR0868276.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34261..35115
                     /locus_tag="ACHUPM_000391"
     CDS             34261..35115
                     /locus_tag="ACHUPM_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0868277.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35306..36586
                     /gene="csiE"
                     /locus_tag="ACHUPM_000392"
     CDS             35306..36586
                     /gene="csiE"
                     /locus_tag="ACHUPM_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR0868278.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36578..37717)
                     /locus_tag="ACHUPM_000393"
     CDS             complement(36578..37717)
                     /locus_tag="ACHUPM_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR0868279.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37877..38767)
                     /gene="hcaR"
                     /locus_tag="ACHUPM_000394"
     CDS             complement(37877..38767)
                     /gene="hcaR"
                     /locus_tag="ACHUPM_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR0868280.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38903..40264
                     /gene="hcaE"
                     /locus_tag="ACHUPM_000395"
     CDS             38903..40264
                     /gene="hcaE"
                     /locus_tag="ACHUPM_000395"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR0868281.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40261..40779
                     /gene="hcaF"
                     /locus_tag="ACHUPM_000396"
     CDS             40261..40779
                     /gene="hcaF"
                     /locus_tag="ACHUPM_000396"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR0868282.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40779..41099
                     /locus_tag="ACHUPM_000397"
     CDS             40779..41099
                     /locus_tag="ACHUPM_000397"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR0868283.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41096..41908
                     /gene="hcaB"
                     /locus_tag="ACHUPM_000398"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41096..41908
                     /gene="hcaB"
                     /locus_tag="ACHUPM_000398"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR0868284.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41918..43120
                     /gene="hcaD"
                     /locus_tag="ACHUPM_000399"
     CDS             41918..43120
                     /gene="hcaD"
                     /locus_tag="ACHUPM_000399"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR0868285.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        10257 a        11944 c        10853 g        10174 t
ORIGIN      
        1 aacaaggaat agtgatgaaa tttaaaaaat gtcttctgcc tgtggcaatg ttagcgtcat
       61 tcactctggc aggatgccag tcaaatgctg acgatcatgc cgccgatgtt tatcaaaccg
      121 atcaactgaa taccaaacaa gaaactaaaa ccgttaatat tatttccatt cttcccgcaa
      181 aagttgccgt agacaactcc caaaataaac ggaacgcaca agccttcggc gcgcttattg
      241 gcgcagtcgc tggcggtgtt atcggccaca acgtcggttc tggcagcaat tccggaacga
      301 cggcaggggc agttggcggc ggagctgtag gcgcggcagc gggttctatg gtgaatgata
      361 aaaccttagt ggaaggtgtt tctttaacat ataaggaagg caccaaagtg tatacctcca
      421 cccaggtggg taaagagtgc cagtttacga caggtttagc cgttgttatt accacaacgt
      481 ataacgaaac gcgtattcag ccaaatacta aatgtcctga aaagagctaa taatcaggag
      541 gagtcatgaa gaaagttttt ctttgcgcca tcttagcctc cttaagctat ccggctatcg
      601 cctcatcatt gcaggatcaa ctctctgctg tcgcagaagc ggaacagcaa ggtaaaaatg
      661 aagagcaaag gcagcatgac gaatgggtcg cggagcgcaa cagggaaatc cagcaagaga
      721 agcaacgtcg cgcaaatgcc caggccgccg ctaacaaaag agcggcaacg gcagcggcaa
      781 ataagaaagc tcgtcaggat aaactggacg ccgaagccac tgcggacaaa aaacgcgatc
      841 aaagttatga agatgagcta cgcagcttag agattcagaa acaaaaactg acgctggcga
      901 aagaagaagc ccgcgttaag cgagaaaacg aatttatcga tcaggaactg aagcacaaag
      961 ctgcgcaaac cgatgtggtg caatctgaag ctgacgccaa cagaaatatg actgaaggcg
     1021 gtcgcgatct gatgaaaagc gtgggcaaag cagaagagaa caaatcggac agctggttta
     1081 attaatcgat gttagtaact tcaagcctat gattcttgaa gataaaaaac cctctgtagt
     1141 aacagagggt tttgttcatt catagtgcag ggattaaaat cattcccact caatggtagc
     1201 tggcggcttg ccgctgatgt catacaccac gcgggaaata ccgttcactt cattgataat
     1261 gcggttggaa acgcgaccga ggaaatcgta cggcagacgc gcccagtgtg cggtcataaa
     1321 gtcgatggtt tcgacagcac gcagagagac aacccagtca tacttacgac catcgcccat
     1381 tacgccaacg gaacgtaccg gcaggaacac agtgaacgcc tggctgactt tgtcgtacag
     1441 gtccgcttta cgcagttctt caatgaagat ggcgtcagca cggcgcagca ggtcacagta
     1501 ctctttcttc acttcaccca gcacacgaac gccaaggcct ggtcccggga atgggtgacg
     1561 gtacaacatg tcgtacggca ggcccagctc cagaccaatc ttacgcactt cgtctttgaa
     1621 cagctctttc agcggttcaa ccaggcccat cttcatctct ttcggcaggc cgcccacgtt
     1681 gtggtgagat ttgatgacgt gtgctttacc ggttgcagac gccgcagatt cgataacgtc
     1741 agggtagatg gtgccctgcg ccagccactt cacgtcttcc agtttcagcg cttcttcatc
     1801 gaatacttca acgaaaacgc gaccgatgat tttacgtttt gcttccggat cgttttcgcc
     1861 agccagcgct gacaggaagc gatcttctgc cggaacgtga acaatgttca gaccaaagtg
     1921 atcgccaaac atatccagaa cctgctctgc ttcgttgaga cgcagcaggc cgttgtcgac
     1981 gaatacgcaa gtcaggtttt taccgatagc gcggtgcagt agcattgcgg ttacggagga
     2041 atccacgcca ccagagaggc cgaggatgac tttatcgtcg cctacctgct cacggatgcg
     2101 ggctacagca tcgtcgataa ttttcgctgg cgtccacagg gcttcacact ggcagatatc
     2161 acgcacaaaa cgctccagca tgcgcatacc ctggcgggtg tgagtcactt ccgggtggaa
     2221 ctgtacgcca tagaagcgtt tttcttcgtt agccataatg gcaaacgggc agctttcggt
     2281 gctggctacg gtgacgaagt cggacgggat agcggtaact ttatcgccgt ggctcatcca
     2341 gacatcgagc agcggtttac cgtctgcggt cagcgcatct tcgataccgc gaaccagtgc
     2401 actgtcgttt acgacttcaa cctgcgcgta gccaaattca cgttcgttag aggcttcaac
     2461 gtgaccgccc aactgcattg ccatggtctg catgccatag caaacgccga ataccggtac
     2521 gcctgcttca aagacatact gcggcgcacg cgggctgttt tcttcagtag tactttccgg
     2581 gccgccggaa agaataatgc cgcttggatt gaagtcacga atttgtgctt ctgtcacatc
     2641 ccacgcccac agttcgcagt aaacacccag ctcacgcacg cggcgcgcaa ccagttgagt
     2701 gtactgagaa ccgaagtcta gaatgaggat gcgatgctta tgaatgtttt ccgtcattga
     2761 cgcttattcc gaggcaagtg aaacagataa tataaatcgc ccgacatgaa gtcgggcgaa
     2821 gagaatcagg agcccagacg gtagttcggg gactctttag taatggtcac gtcgtgaacg
     2881 tggctttcct gaatgcccgc accgctgata cgtacaaact ccgctttagt acgcagttcg
     2941 tcgatagtac cacagccggt cagacccata caggagcgca ggccgcccat ctgctggtga
     3001 atgatctctt tcaggcgacc tttataagct acgcgacctt cgataccttc cggcaccagt
     3061 ttgtcggcag cgttatcgct ctggaaataa cggtcagagg aacctttgga catcgcgccc
     3121 agggaaccca taccacggta agatttgtaa gaacggccct ggtagagttc gatttcaccc
     3181 ggagattctt cagtacccgc cagcatggaa cctaccatca ccgcgcttgc gccagcggcg
     3241 atagctttgg cgatgtcgcc ggagaagcga ataccaccat ctgcgataac cggaataccg
     3301 gtgccttcca gggcttcaac tgcgtcagca acggcagtga tctgcggaac acctacgcca
     3361 gtaacgatac gagtcgtaca gatagaacca gggccgatac cgactttaac cgcgctgcaa
     3421 ccagcttctg ccaaagcgcg tgcacctgca gctgttgcca cgttgccgcc gatgatttgc
     3481 agatccggat atttagcacg ggtttcacgg atacgctgca aaacgccttc tgagtgaccg
     3541 tgggaggagt cgatcagcag aacgtcaacg ccagcggcaa ccagcgcgtc aacacgctct
     3601 tcgttacccg cacctgcgcc aactgcagca ccaacacgca gacggccttg ctcgtcttta
     3661 caggcgttcg gtttacgttc cgctttctgg aagtctttca cggtgatcat gccgatcagg
     3721 tggaattcgt catcaaccac cagcgctttt tcaacgcgtt tttcgtgcat ttttgccagc
     3781 accacttcac gggcttcacc ttcacgcacg gtgaccagac gctctttcgg cgtcatgtaa
     3841 acgctaaccg gctggttcag gtcggtaaca aaacgcacgt cacgaccggt gataataccc
     3901 accagttcgt tttcttcggt aacgaccgga tagcccgcaa aaccgttacg ctcggtcagt
     3961 tctttcactt cgcgcagcgt cgtggttggc agaacagtct gcggatcagt caccacacca
     4021 gattcgtgtt ttttcacacg gcgaacttct tctgcctggc gttcaatgga catgtttttg
     4081 tggataaagc cgataccgcc ttcctgagcc agagcaatag ccaggcgcgc ttccgttacg
     4141 gtatccattg ctgcggaaag catagggata ttcagacgaa tagttttcgt cagctgggtg
     4201 ctgaggtcag cagtattcgg cagaacggta gaatgagcag gaacgaggag aacgtcgtca
     4261 aacgtcagag cttctttagc gatacgtagc atgggcaata tctcgaccag agtggttaat
     4321 aaatattgcc gcggcattat acagagcgta accgattgca tctacccctt tttgcaaaaa
     4381 atgcttgcta tccccgaagg gcgggttact atcgactgaa taacctgctg atttagaatt
     4441 tgatctcgct cacatgttac cttctcaatc ccctgcaatt tttaccgtta gtcgcctgaa
     4501 tcaaacggtt cgtctgctgc ttgagcatga gatgggacag gtttggatca gcggcgaaat
     4561 ctctaatttc acacaaccgg cttccggtca ctggtacttt acactcaaag acgacaccgc
     4621 ccaggtacgc tgcgcgatgt tccgcaacag caaccgccgg gtgaccttcc gtccacagca
     4681 tggacaacaa gttttagttc gcgccaatat tacgctctac gagccgcgcg gtgactacca
     4741 gataatcgtt gagagtatgc agccggccgg tgaagggctg ctgcaacaga agtacgaaca
     4801 gctcaaagcg aagttgcagg ctgaaggttt gttcgatcag caatacaaaa aaccacttcc
     4861 ctcccctgcg cattgcgttg gtgtgatcac ctcaaaaacc ggtgctgcgc tacatgatat
     4921 tttgcatgtg ttaaaacgtc gcgatccttc tctaccggtg atcatctacc ccaccgccgt
     4981 tcagggcgat gacgcaccgg ggcaaattgt tcgcgccatt gaactggcga atcagcgcaa
     5041 tgagtgcgac gtgttgatcg ttgggcgcgg cggcggttcg ctggaagatt tatggagttt
     5101 taacgacgaa cgcgtagcgc gggcgatttt tgccagccgc attccggtcg tcagcgccgt
     5161 cgggcatgag acggatgtga ccattgccga ttttgttgcc gatctgcgtg cgccaacgcc
     5221 gtctgccgcc gctgaagtag tgagccgtaa tcagcaagag ttactgcgcc aggtgcaatc
     5281 gacccgtcaa cggctggaga tggcgatgga ttattatctc gccaaccgca cgcgtcgctt
     5341 tacgcagatc catcaccgat tacagcaaca gcatccgcag ctccggctgg cacgccagca
     5401 aaccatgctt gagcgcctgc aaaagcggat gagctttgcg ctggaaaatc aacttaagcg
     5461 taccgggcaa cagcagcagc ggttaacaca gcggctgaat cagcaaaatc cacagccgaa
     5521 gattcatcgc gcgcaaacgc gcattcagca actggaatat cgtttagcag aaaccctgcg
     5581 cgtacagctt agcgccacgc gtgaacgttt cggtaatgca gtaacgcacc tcgaagccgt
     5641 aagcccactg tcaacgctgg cgcgtggata cagcgttact actgctactg acggcaatgt
     5701 actgaaaaaa gtgaagcaag ttaaagcggg tgaaatgcta accacacgtc tggaagacgg
     5761 ctggatagaa agtgaagtta aaaacatcca gccagtaaaa aaatcgcgta aaaaggtgca
     5821 ttaagccagc acgaattcaa cgcgtttttt cgatatcaag ccatgcccgt gctgacagaa
     5881 gtaatctact gcaccgcagg cttttaacac ctgcaacggt tgatggcagt ctggacaaag
     5941 agctttcatt tcaataattt ccccgcagga tgggcaacgc gcatggccgt tatcctgatc
     6001 aagcacatgc tgacattgtg gacaatgcag ttccatatga attcctccgg tagccatccc
     6061 tctacattca tagagggaat ggcagataaa atacttacgg ataattattt atttttcttg
     6121 atgtgcttca tcagacgctt acgtttacgc atctgggttg gcgtcagggt gttacgcttg
     6181 ttcgcatacg ggttttcccc ttctttgaac tgaatacgaa tcggcgatcc cattacgtcc
     6241 agcgatttgc ggaagtagtt catcaagtag cgcttgtagg aatcaggcag gtctttcacc
     6301 tgattaccgt gaatcaccac aatcggcggg ttataaccac cagcgtgggc atatttcagc
     6361 ttcacacgac gaccgcgtac cagcggcggt tggtgatctt caacagccat cgtcatgatg
     6421 cgcgtcagca tagaggtccc cacacgacgg gtggagctgt catacgcttc acgtactgat
     6481 tcaaacaagt taccaacacc actgccgtgc aaggcagaga taaagtgcac acgagcaaaa
     6541 tcgataaagc ccagacggaa gtccagcgtt tctttcacct gctctttcac ttcctggctc
     6601 aggccatccc acttattcac cacaatgaca agtgagcgcc cactattgag aataaagccc
     6661 agcagcgaga gatcctgatc ggaaatacct tcgcgcgcat caatcactaa catcaccacg
     6721 ttggtatctt caattgcctg caacgttttg attacggaga atttctctac agcatcggtg
     6781 attttgccgc gtttacgtac gccagcggtg tcaatgagca catactcacg tccatcgcgt
     6841 tccattggaa tgtagatact gtcacgcgtc gtgccaggca tgtcataaac aacaacgcgc
     6901 tcttcaccaa gaatacggtt agtgagtgta gacttaccta cgttcggacg acccacaatc
     6961 gccagtttga tcggcagact ttgcgggtcg aagtcgtctt cctcttcttc ttcgccgttc
     7021 tcttccgctt caaattgcgc ccagtattca gcgtcttcgt cgacttcctc ttgcggtgcg
     7081 agatcttcca tccacggcag cagcacacgc tccagcagac ttaatacgcc acgaccgtga
     7141 gatgcggcga tcgggtagat ttcacctaaa ccaagcgagt agaaatcaac cactgcctga
     7201 tcgggatcca gaccgtcagt tttgtttgcc accaggaagg tcggtttttc acgagagcgc
     7261 agatgtttgg caatcgcttc atctgccggc atcaagcccg cgcgcgcatc caccataaac
     7321 agtacgacgt ccgcttcttc aatcgccagc agcgactgtt ccgccatgcg ggtttctaca
     7381 ccgtcttctg tgccatcaat cccgccggta tcaatacaga taaactcacg gccttcaatt
     7441 tccgcacgac cgtacttacg gtcacgagtc agacccggga aatccgcaac cagcgcatct
     7501 cgggtgcgag ttagacggtt aaataacgtg gattttccta cgttagggcg cccgacaagc
     7561 gcgaccacag gtaccatgtt taaagcctca tttttataaa tcatcagaca acgcacgcta
     7621 tattcgcgtc gttgttaaaa acaggaaaac ggcccctgtc caggagccgt tttcaaagtg
     7681 aacgacagag acgattaacg tgtaatagag tacacggttc cgtcttttgc ctggatcagc
     7741 agtttgccgt cagcggcaac cggttcagtc tggaaaccgg aactatcaac tttttgctgg
     7801 gcaacgaaac gaccatcttc gacgttaatc cagtgcagat aaccttcact gtcacctacc
     7861 accaggttgc cattatacag caccggcgaa gtcagcaggc gatgcagcag atcgctttgt
     7921 gtccacagcg taacgccgcc atcaatggtc aacgccatca cccggtcatt ttgatcgacc
     7981 agatagatgc gattgccgtc gacgatgaaa tcattcaccg aacccagttc gcgtttccac
     8041 ataatctgac cactgcgcag atcaagcgcc gtcaggttac cattataggc cagagcgaaa
     8101 acaacgccgt taacaacgac gggagtcgtg tcaacatcgc tcagacggtc aatttcggta
     8161 gaaccggtcg cctgggaaat acgctgctgc caaatcatct ggccctgttc catcagcact
     8221 gcgctgacgc gaccattatc gccccccacg acggccgcac caaaagccgt tgccggcgca
     8281 gactcgccac gcaaagagag cgaaggcata tcgaggttaa ctgtccattt gacagcgccg
     8341 tcagcttcgt tcagcgcttg taactgaccg ttactagtgt ggattaacac cagaccgtcg
     8401 ctgaccaccg ggcgagaaag tgcttcaccc gcgactttag tttgccatgc cacagtacca
     8461 tcgctggtat tcagcgcgta aacctgcgcc ttttcgctgc caatgtagac atgcccacca
     8521 gacacggtca caccgccaga aagtaatgca ggctctttag agaaccagcc atctttctcg
     8581 gccaggctga cagaccagat ttctttgcca tcatccgcat tcagcgcttt taccaaacca
     8641 gcgcggtccg ctgcatagac aacgttgtcc gccagtgccg gatgaagatt ggaatagaag
     8701 ttgccaatgc cgctaccaac ggaagtgctc cacgccgtgg tcggcgtaaa ctggttttca
     8761 acggttggca atggggacat ctttaccaca tcttcttcgc tgttaaacag cgaacagccg
     8821 cttaaaaggg taacggaaag cagtcctggc agcagtaatt tacgcaattg catcgggtcc
     8881 ctctcagatg gacaaattat taattttcat ctgcatcatt tcgctcagtg ccggagtaac
     8941 atcgcttttc acgcctgctt cccatgcact acgcgcacct tgcttatcac ctttgctcaa
     9001 caatgcttta ccacgcaggt cggcaacaat ggcagcccac ccttcacctt tgatggtatc
     9061 aagggttttc agcgcggcat cagcctgctt gagctgtacc tgaacgcgag caagacgcag
     9121 atttatcacg gctttgagat tttcatcact cgtgtctgcc agcccctgtt gtaactgggc
     9181 ggcagctttc tctagttcat ttttgtcaac aaattgctgc gcaagttcca aagaagccag
     9241 cgcaccataa gtatttttat tttcagcagc aaatttttcc gccgccggga tgctatccgg
     9301 tttgccttcg ctcactgcgg taaccgcatt ttgataggca agagaagcgg agcgtgcaga
     9361 atcaacctga tggctgttcc agtagcgcca gccaatcagt gcgccaacgc ccaaaatcac
     9421 cccaacagcc agtgctttgc cattttcagc aaaaaagcgt ttaaccgctt ctacctggtc
     9481 gttttcgttc tcgtaaattt ccacgctgtc cttctccttc cttaacccag taacgtgcgc
     9541 aaatgcgcgg ctacgctatc ctgcgcaact gccgtttgct caccagagcg caaatccttc
     9601 actactgctg tgccgttagc cacttcagac tcacccagca ccacagcaac gcgggcaccc
     9661 catttatcag cacgggcaaa ctgtttctta aagttgccgc cgccgtggtt ggtcatcaat
     9721 ttcacgcccg gtaattcatc acgcagacgc tcagctaatg ccatagccgc agattgtgta
     9781 tcagcacctg aagccaccag gtatatatcg acaacaggat cggctttaaa ttccggatta
     9841 acggcctgta ctaacaatac aagacgttcg aggcccatcg caaaaccgac tgccggtgtt
     9901 gcacgaccgc ccagttgttc caccagaccg tcataacgac cgcctgcaca cacggtgccc
     9961 tgggagccga ggctgttagt cacccactcg aaaacggtac ggttatagta atccagacca
    10021 cgcaccagac gctggtttac ggtgtaagcg atccccgcgc tctcaagcag tttgcacaga
    10081 ccggcaaaat gctcacgaga ttcctcgtcc agataatcac ctaatgccgg agcgtcgttg
    10141 agaagcgcct gcacttccgg attttttgaa tccagcacgc gcagcgggtt agtgtacatg
    10201 cggcgtttgc agtcttcgtc cagcttttct ttatgctgct caaggaatgc caccagcgca
    10261 tcgcggtaat tggcgcgtgc ttccagcgaa ccaatagagt tcagctcaag agttacgtgt
    10321 tcgaagatac ccagcgcacg ccaccagcgg gcagtgagca taatcagttc agcgtcgata
    10381 tccggacctt gcagaccgaa aacttcgcag cccaactgat ggaactgacg ataacgccct
    10441 ttctgcggac gctcgtgacg gaacatcggc ccgatatacc acagacgctg ttcctgattg
    10501 tacagaagac catgctcgat gccggcgcgt acacagcccg ccgtcccttc agggcgcaga
    10561 gtcaggctgt cgccattgcg atcctcaaag gtgtacatct ctttttcaac cacgtcggtg
    10621 acttcaccaa tcgcacgttt gaatagcggg gtctgctcta caatcggcaa gcggatttca
    10681 ctgtaaccgt agctgccgag cacgtttttc agtgtgcctt caatgcgctg ccagatggcc
    10741 gtttcgccag gcaggtaatc gttcatgccg cgaatggctt gaatgttttt tgccacgttt
    10801 attctctttc tgaatataaa aatgaaccct caacgcttcc ctcaatgttt cgggagccat
    10861 gcgggttcaa tcatacacgg gaagcgaggc gcttcccatc acgttattat ttttcaacct
    10921 gctgaacgtc aattcgacgc gcttcgtcca gctgactggc tttcgcacga atgcgtgctt
    10981 ccagctggtc gatcatatcg ttgttgtcca gacggtcttt gcgcacgcca tcttcataga
    11041 ggccgctttt cttgttgccg ccggtgacgc cgagtgtaga aaccagcgcc tcacctgggc
    11101 cattcaccac gcagccgata atcgaaacgt ccatcggagt gatgatatct tccaggcgtt
    11161 gctccagcgc gttaaccgta ccgataacat caaattcctg acgcgaacag gtcgggcagg
    11221 cgatgaagtt aatccctcgc gaacggatac gcagcgattt caaaatatcg aaaccgactt
    11281 tgatctcttc gaccggatcg gccgccagcg atacgcgcag cgtgtcgccg atgccttccg
    11341 acagcagcag acctaaacca atggcggatt ttactgcccc gctgcgtgca ccaccggctt
    11401 cggtgatccc cagatgcagc ggctgatcga tctgttttgc cagcaaacga taagactcaa
    11461 cagcgaggaa gacgtcagac gctttcacgc tgactttgaa ctgatcgaag ttcaggcgat
    11521 cgagatgatc aacatgacgc atggcggatt ccagcaacgc ctgcggcgtc ggttcgccat
    11581 acttttcttg cagatctttt tccagcgatc cggcgttaac gccaatacgg atcggaatgt
    11641 ttttatcgcg cgcacagtca accaccatgc gaatacgctc ttcattaccg atattgccag
    11701 ggttaatacg cagacaatcg acgccgtatt ccgctacttt cagcgcaatg cgatagtcga
    11761 agtggatgtc agccaccagc ggcacgttaa cccgctgttt gatgagtttg aacgcttctg
    11821 ccgcgtccat cgtcggtacg gagacacgga cgatatcagc gccaacgcgt tccagcgcct
    11881 tgatttgatt gaccgttgct tcgacatctg tcgtacgcgt attggtcatg gactgtacgg
    11941 cgatgggagc accatcgcca atcggcacat tcccaacgta aatacgtgtt gattttctac
    12001 gttgaattgg agcctggtta tgcatgaaaa atctcccgcg ttacccgtct gttactgcgc
    12061 cggtgattgt tcggcattga gggtcagacg cgcaacctgg ttagttctga taaaacgact
    12121 cagatcgaca ggtttccctt gatactggat ctgtactgcg gctggcgcac caattttcag
    12181 tttatacggt gcctggccgg ttaaattcaa attaccgtct ttacgctgca tcccgctaaa
    12241 caatttttta ccggtagcat cagtgacctc cagccagcaa tcggcagtaa agttcatcac
    12301 cagcgcattc ggatcagccg ccggcgtggt cacgccagcc tgatcggttg gcaacggcgc
    12361 agcaccatct ggcgttgttg ctgccgttgg taccggggtc gcggcggtat caacatttgc
    12421 ctgcgaaggc gaaacaaccg cattctgttg cggatcaaca gctggtgctg gcgcagttac
    12481 ggcaggtgtt tgcgtgttgg ttgcggtagt atccacagac gctggcggcg tggacgttgt
    12541 agccgggtct gtagttgtcg acgtatttaa cgggacactc tgcccctgct cgctattact
    12601 gctcagttct gccgaagatt gatcggccat agtggtgatc tcttcctgct gagctttgtg
    12661 gtcttgccac caccaggcac cgctcaggcc gataaccaca aacaacacca gccaggtgaa
    12721 ggtcatcagc cagccatcac gttttttgcg gcgtttaccg agggaaaaac tctgcattgg
    12781 cgcaactttt gcagcccgta gtggagcctg cttttccagc cctggcagca gttcttcttc
    12841 tggaatatgt accagacgcg cataagagcg gatatatccg cgcaggaatg ttgaagcaag
    12901 atcggcgggt gccttatctt cttcaatgtc gcgtaccgtg gaaaccttca ggcaaagtcg
    12961 ctcggcaacg gcctgctgac taagtcctag ttgttcgcga gcattacgca ggcgagcgcc
    13021 ggtagtaagt gcttcatttt ggtcgtgcgt ggcttcagta ttcattcgct gcaggtacgt
    13081 ttaaatgaga attgagatgc cggtgaacca tcatgctcac ccacaccgcg aaacatccgt
    13141 taagttaacc gttatcatac agtataagac cgtctgtgtg ctcgtgacaa agcccacatt
    13201 cactaaggct aacttactgt tgcatcgtta catactgcct taaagtcagc aaaaacgcac
    13261 cgttaatatt gaccagacaa ttgcaactta atacttcatt cataaaacat tacgccacgg
    13321 tacataaagt aaccgtggcg taatggctat cagaccgctt taatgtcgat ggcttcaccc
    13381 tgcatccgtt tacgcagggt acgtttcgta cggtcgataa catcgcccgc caactgacca
    13441 caggcagcat cgatatcatc accacgagtt ttacgcacaa tagtggtgaa accgtagctc
    13501 atcagcactt ttgagaaacg gtcgatacgg ctgttcgagc tgcgtccata cggcgcaccc
    13561 gggaacgggt tccacgggat caggttgatc ttacacggcg tatctttcag cagttccgcc
    13621 agttggtgcg cgtgttcagt gccgtcgtta acgtggtcaa gcatcacgta ttcaatagtg
    13681 actcggccct gattggcgtt ggatttctcc agataacggc gcaccgcagc aaggaacgtt
    13741 tcgatattgt actttttgtt gatcggcaca atttcgtcac gaatttcgtc gttcggcgcg
    13801 tgcagggaaa ttgccagtgc aacgtcgatc atatcgccca gtttatccag cgccggaact
    13861 acaccggaag tagaaagcgt gacgcgacgt ttagacaggc caaaaccgaa atcatcaagc
    13921 atgatttcca tcgccggaac gacgttgttc aggttgagca gcggctcgcc catgcccatc
    13981 atcactacgt tagtgattgg ccgctgaccg gtgacttttg ctgcgccgac gattttcgcc
    14041 gcccgccaca cctggccgat aatttccgac acccgcaggt tgcggttaaa gccctgctgg
    14101 gcggtggaac agaatttaca ctccagcgca caccccacct gcgaagagac gcagagcgtg
    14161 gcacggtcgt cttccgggat atacaccgtt tcgacgcgct gatcgccaac ggcgatcgcc
    14221 catttaatgg tgccgtcgga tgaacgctgt tcttcaacca cttccggtgc gcggatctcc
    14281 gccacctctt tcagtttgcc gcgcaacact ttgttgatgt cggtcatctc atcaaagttg
    14341 tcgcagcaat agtgatacat ccacttcatc acctgatcgg cgcggaaggg tttttcacct
    14401 aaatctttaa aaaactcccg catctgctga cggttgagat ccagcaggtt gatttttcca
    14461 tctttcgtgg tgacgttttc aggtgtgact aattgttcag acatatgcta ttccggcctc
    14521 gttattacac gttatggccc ctggagggtt gaaaaaagaa acgccccggt gagcggctgc
    14581 tcgtccgggg gcgctgcatt gtacaaattc tggcgcacgg atgccacgtt tgcacgcggc
    14641 atttacgaaa ttattaacgg gtgcgcgggc acacttcgcc ttcgccgaag aaataagcga
    14701 tttcgcgagc ggcagattcg acggaatcag aaccgtgggt accgttttcg gtcaggctgt
    14761 cagcgtaatc agcgcgcaga gtaccggcca gtgcgtttgc cggattggtc gcgcccagca
    14821 gatcgcggtg acgctgaacg gcgttttcac cttccagcac ggaaaccacg atcgggccag
    14881 aggtcatgaa ttcaaccaga ccatcaaaga acggttttcc atcgtgttca gcataaaagc
    14941 cacgagcctg ttcaacggtc aggtgcagca ttttggtgcc gacaattttg aaccctgcag
    15001 cttcaaagcg cgcaaagata ttaccaatga cgttttttgc taccgcgttc ggtttgatga
    15061 tggaaaaagt acgttcaata gccattttta cctctgtaaa ttgttctgtt gttgtctgta
    15121 ccagcgtacg agatggcgcg gattataatg agcaacaggg ccgttgacta ttgatgaagg
    15181 taacattttt ttaaaataaa atgagtttta gcaacaatca gggacaaaac agactattgc
    15241 atgacaaatt tcactgtcgc gatttgcccc acgtcatcca tcaccagcaa ttgataatca
    15301 cctttatccg tcaaatgcag ggtcacgttg cgcccgcgtt cagttaacgg ttcgccattc
    15361 agaaaccacc agcgttcacc tgcccctcca cttgattgca acggcaaagt cgcttccgct
    15421 gcgcccggta aacgtttaat aatcgcgcca tcgcgaacgc ctgtcagctg cagagggagc
    15481 tgggcatcgt gaccgtatgg cgggcaaatt gtcgaggccg gtggtaagcg cacagcgcga
    15541 cgttctgatg cgggcagcca gggttccagc ggtagcggcc agacatttac catttcttgc
    15601 ttcgcctgcg ggcagtcagc ggcgacgcgc tgccctttgt catccagcca gatggggaag
    15661 cgaatgccat taatcccttc ctgctctgac agtaacagcg ttggcggctg gctgccatcc
    15721 agcagccagg ttgccaggcg gcggcgacag ttaccgtcac cttccggcaa agactgtccg
    15781 cccggccagc agataacgcc acgagtgact gagttcgggc gcgggtcttc cggcagattc
    15841 gcactgcgcg acagtaagat attattgacc tgattcaaca atggtacggc actggcaaag
    15901 ccaaactgac caacaacggg cgtgccgtca ggtctgccag tccagatccc aatgacatag
    15961 cgagcgttaa ccccaatcgc ccaggcgtca cgatagccat agctggtgcc cgttttccat
    16021 gccagtgggg cgacgcgcgg caaggcacta tccggcaagg gttgcgcttc atcagccata
    16081 atccggcgaa tgatccacgc cgcccccgac gacattaaag gccgttcaag cagcggatcg
    16141 tcaggctgta agcgcaattt gcctgccttg ccgtggcgag caaacgcggt atacgctgcc
    16201 gccatatctt ccagttttgc accagcgccg ccgagaatga gtgaaagatt cggcgcagcg
    16261 ccgttgggca aatataacgg caatccaaca ttgcgtaact ttgccgcaaa ccgtttcggt
    16321 ccataggctt ccagcacctg cacagcaggt aagttcagcg agcgcaccag cgcctcgctc
    16381 atgctgatcg ggccatgaaa accgctatca aagttacctg gtcgataatc accggtgcgc
    16441 cgggggacgt cttgcagcag tgatgccggg tggatcaagc cttcatccag cgccagacca
    16501 taaacaaacg gtttgagcac cgatcctggc gatcggatcg cattgaccat atcaacatga
    16561 ccaaagcgtg aatcatcgtt gagatcaacc gatcccaccc agccgcgaac acgcatatcg
    16621 gtatgatcaa ccacgatcat cgccagtgag ctgcgcggtg gcaaccgccc tttccagttt
    16681 tgcgccagtt cttccagacg tcgttgaaga ccggcatcca gcgtagtaac gattttgtcg
    16741 cttttgcttt taccgagcat catgcgcgaa aacagcggtg ccagttgcgg catttgtcgg
    16801 ggtgccagcc agatgggttc ttcccttgac tcttttacct gctcacggga ccacacacct
    16861 tgcgcagcca tccgttcgag cactttatta cgcgcggctt cggcacgctc cggccaacga
    16921 tccgggcgaa gacggctggg cgcttgcggc aaaaccgcca gcattgccgc ctcggaatag
    16981 cttaaattcg caggcgactt tccgagataa gcccaacttg ccgcaccgat cccctgcaac
    17041 gtaccgccaa acggagcgcg gttaagatac aaggtcagaa tttcacgctt agacagatgc
    17101 cattccagtt gcaacgcgcg ccagagctgg cgaattttgc cgccaaatgt tttggggtga
    17161 ggatcaagca gacgagcaac ctgcatagtg agcgtgctgc caccggaaat aacccgtccc
    17221 gaagtgagat cttgccatgc tgcgcgcgcc accgagaatg gattcacccc cggatgcttc
    17281 cagaaccagc gatcttcata attgatcagc gcttcaaggt aacgtggaga aacatcttcg
    17341 attgttaccg gataacgcca gatgccgtca gcatcggcga agcgccagag cggcgtacca
    17401 tcctgcgcca cgaccactcg tgcgggattg acttcatgca gcggtagagg ccagagttta
    17461 tctgctcccc acgctgcaag aaaaaggaga aatggcgcag ctgccagcgt tatccagcag
    17521 ccgcgtttgg ttaacaagcg aggcatttac ggtcggacaa tcagcagatc ttcagccgcg
    17581 ccggtcgccc gccattgggg aacatacatt gattccacca tcggttgcgg tacctgatac
    17641 gttcccggcg tcaccgcccg cgccagatac accagcgtta ccggttggta ttcatcaacg
    17701 gcaaccgccg ccacaaagcg atcgtcacgg aactcaatgt gcttaatact cgcctgctgc
    17761 atctggttca gtaagttctg cacttcgcca ccactttgct ccaggctggc gctaccgttc
    17821 gccagattct ggttttccag ttccagaccc acaggcagca gatccacgac taacgcatcc
    17881 ggcacgctgt tactggcttt tacctgcaac cacaccagca ccagatcgcc gctacgtaac
    17941 gagtccagcg atttgctctt accatcagta ccaagaatat gacgctcgat ttgcagcaca
    18001 ttgttcgccg gtaaaggtgc ggattgcgga taaccgctgg catccatacg caaccataac
    18061 ggctgatcgc cactgttgct cacctgcaag gtgacaagtt gatcgctatt cagattgctg
    18121 ttttgcgctt tctcgcctgt cagcggctca gctgagaaag aggtttgcgc ctgccattta
    18181 cccggtaaat cctgaatcgt acgggcagcc aggaacaacg cgttactttc ctgcgtcgat
    18241 agccagcgtt caccaaacgc ctgctgcgaa agtgtgttca gcaaagtgta ctgctcatcg
    18301 ggtagcagtt tattttcttc cagcaaggag agcattaacg cgttgtcgcg cagtggacta
    18361 ccgtaatcac ccagccatat ccgctcatca ctatttctcg gcgttttcag cgccagcgca
    18421 atcgcttctt caccacgcgt cgcatcaccc atggttttca gcgcaatgcc aagttgcagc
    18481 agcggtaaac cagaagcggc atctgcacga tgctcccaga tttcacgcag cgcacccagt
    18541 ggtgcttttt gctgacgggc cagcaccagc gcagcgtaag actgaacggc gaatttactg
    18601 gctttgagat tatccgcgta cgggatcgac atcatgcccg gatcttgtaa atagcgcagc
    18661 agacgctcat taccccggtt aatggcgtct gtcggcacgc tgtaaccctg ttcgcctgcg
    18721 cggaccagga aatccatcac gtaagccgtc agccagtact cttcgtcacc gtttttatcc
    18781 cacagcgcaa agccgccgtt atcacgttgc atttgcagca aacgggaaat gccgatatcg
    18841 accgatgcac ggcgtttctc atcactgtcg cctttgatgc ccaacgcctg cagttgggct
    18901 gcgttggtat aaagtgacgg gaacaggccg ctggcggttt gctcaagaca gccgtacgga
    18961 tacgctttta actctttgat ataacgtgca atgttcagcg gtggtttgcc gctcaacaac
    19021 aattgccctt ccagcgtaac aggcgagaag ttttgcaatc catccgccgg aatcgcccat
    19081 gtctcaccag gctgtaacgc cgtaccgtaa ttaaccgttt gtgccgggaa cgccggacgg
    19141 acgccgattt tccactgctt atgctgatcg gcaacggttt cacccggtaa cgctaacccg
    19201 ctaatggtgg cctgaatttc tccatcgcca taacccggca atgctcgcac cgggataaac
    19261 agcgtagtac gcacacctgg cgctaattca acggctgcgg gtgaatcgct gaccagttca
    19321 agcaaaccac tggcggtcag ggcaacgttc agtttttgcg gtttatcggt aagattagtg
    19381 atatccagcg tcagacgcga ggtatcgcca ctcgccataa agcgcggcat gttcagttca
    19441 gcaatcaccg gtgcggcaac tatcactttg ctttcgttgc taccgaaatc atctgccgtc
    19501 caggcttgcg ccatgacgcg caattcaccg ttaaaatcgc caatcggcag tgtaaccgag
    19561 ccttcgccct gttcgttgag cgttaccgac agcgcctgct gcgcgacaat attgacgtga
    19621 ttgaccggcg gtttaccacc acgtttcagc tcatcaccat cgccaccgaa acgcagagct
    19681 gccagacgcc cctgaccttc aataacctga ccgtaaatat cgtaaatgtc tgcgccatag
    19741 cgtttctgac caaagaacgc ctgccacgga tctggcgtaa cgtagtcagt aatattcagc
    19801 acaccactat caacggcaga caccagcaca ttcacctgtt taggcttctc gccattttta
    19861 gtgctggctt taattttcac ggttaatggt tgattgggac gcatttttgc tggtgtttcc
    19921 agcgccagat cgaggcgacg gttttcatca ccaagcggca gatgcaacac accaaccgcg
    19981 cgttttggcg tcgcggagcg agatttatcg ccaggacgca ccaccagcgt actcaaataa
    20041 agatcatgac gattccaggt tttatcgacc ggaatcgtca gatccagccc ttgagccgga
    20101 acatcaatct cttgccacca cagcggccct tcactggact cgaccatcgc ataaccttta
    20161 cccgccgttg gcgcggcaat atgcaactta atggtatcgc cggggcgata actggcttta
    20221 tctagtttca gcgtgacacg gtcgggtcgc actgcgccgc tcccgtcgct gttgtcctgc
    20281 cagctatagc cagcccaaaa acgcacgcta ctgaccgctt cattcggcgc tttgacttcc
    20341 agacgataag cgccccactc taccgggaaa ctgaccttac cggtttcatc cgcctgaaga
    20401 tccagagttt gttcattttc gatcagatct ttttgatcaa actgtgactg ccagccttca
    20461 tcttctgacc agttccagta gtaatcgcgg cgttcgcgaa tcaggcgcac ctgcaagccc
    20521 gacacggctt ttttcacgcc ttgcgcatcg ctataaacga tgtcaaaagc ggcgttactg
    20581 ccttcatcaa caatgggctg tttcaccgtg ctgtcagtgc gataatcgta aaccgatttc
    20641 aaggcgaact gcggacggag ccccggcaat gcatcggcag gccagatagc ctgctcagcg
    20701 cggcgcgtca ccgggcgacc gcccgattcc agcaaactgc cctggaaaat aacctgcaat
    20761 ggggaatgcg tttccttcca ctggctttct gtagaaactt cgccgcgccc tttatcatcc
    20821 agcgtcaact gaacttcatc cagcgtgcgg gaaaggtttt cggcagctat atcgccgaat
    20881 tcaaaaccag gtaaggctga cacagcctca cgcagtgggc gcaggaaaag ttgcccttgc
    20941 aaagtattac cattagcagg cgcaccgtac aggtagtatc ccaccacgga gaatttcact
    21001 tcatcattcg gcgtaagcgg ggttttctca ccggtcagat tcagcgccat acgctctggc
    21061 ataaaatctt cgacgtggaa atcccacatc cggtactggt tatcgcccgt gttggcgcga
    21121 atatgccaca taccggttgc cgcattgcta tcgagtggcc aggtaaagtg gtagaggcca
    21181 ttctccggct gactaacgac gttcctgagt acctgcccgt caggtttaat cacgtctaac
    21241 ttgatgggtt gatcgggcaa tgctttaccg tctgcatcgc gcagtaaacc attgaggatt
    21301 accgtttcac ctgggcgata aagatcgcgt gggccaaaca tgaaaaactg tttgctatag
    21361 cctggcgcgc cagcaatgtt aaattctgct aagtccagcg ccggaagttt taaatcgagt
    21421 agcgttgtct gaccgtcttt acgtgccagc agtaatgccg catttttatc attttccagc
    21481 tgcacatgcc cctgagcgtc actggttgcc tgagtcagag tctgtccttt ctcatttaat
    21541 aaagagactt caattccttg ctgggccgcg ccgttttcca gactttgggt aaagatatcc
    21601 agacaattgt gataacggtg agctgaaacg ccgatatcac ttaacgtaaa cagcgtcgcg
    21661 ggattactgt aatcgtaacg tccagcctga ttcatcacag ccagatacac gcccgcctgc
    21721 tgaagcggtt tgatatcgcc cagcggcagc aataattttt cacgggtgtt acgcgcagga
    21781 ttgagatcaa accgtccggt gtagaccaga tccgccatct gcagcagttt gtctgactgc
    21841 cagttcgcca gcgaattgcg gtattcccat tggctaatga atgctggcag agattctggc
    21901 ttaacgcgga agaagttaac atcgacatta ttaacgttga gcgccattac cggcagccct
    21961 tcaacgactt tgccaggcag cagcgaacca cggctggcaa aaccgacgct gggttggatg
    22021 tcgcgggtag ttatagtttt ttcgtaatct ttactgaagg ttgcgttgtt gagtgctttg
    22081 acctccttgc caatagtaac gatcaaatca cgtttcggtt cgaggtggcg taaacgtagc
    22141 tctttaagat tatctgacag ctcccaggca ccatccactt tgccgctttt tttatcgacg
    22201 acatgaataa cgcgtgagaa atcctgatcc gggtcgagag ggatggagaa cgtcagcacc
    22261 agcgtggctg caccatcaag ttggacttca gagagatcca gcaatgtcag caccttaccg
    22321 gcactctgtt gggccagttt ttgtctctcc ggcacggaga gttttgctga actcgcgttt
    22381 tctgaagagg ccgctttagt aacttcagaa ggcgcatctt ttttcaccgc tgttggcgcg
    22441 ttatcgttgt tgtcgcaccc tgccagcgcc agcattagca tgcaggcggc tacgcgtaac
    22501 tttttcattt ttcatccctg ccacaatggc ccgttagcaa cgtcgaataa ttattatgcg
    22561 tgagaattta cgttatgtaa ttcagtatca cgcccataca atccgaattt tagtgagaat
    22621 tgtggctcag acagccaaat cagcctcttt cgcttgtcgc ttttcactaa acatccgaca
    22681 atttagccta accccggcaa aaatggagat gcctatgtcc acgacatggt ttgtaggagc
    22741 cgactggctc gccgaacata ttgatgaccc ggaaattcag attatcgatg cccgcatggc
    22801 gtcgcctgga caggaagatc gtaacgttgc tcaggagtat ctgaatggac atattcccgg
    22861 cgcagtgttt tttgatatcg aagcgctttc tgatcacact tccccgcttc cgcacatgct
    22921 gccgcgcccg gaaacgttcg ccgtggcgat gcgtgaatta ggcgttaacc aggataagca
    22981 cctgattgtc tatgacgaag gtaatctttt ctcagcccca cgagcatggt ggatgctgcg
    23041 cacctttggt gtagagaaag tgtcgattct ggggggtgga cttgcaggct ggcagcgcga
    23101 tgatctgctg ttagaagaag gtgcagtaga gctgccggaa ggagagttta acgccgcgtt
    23161 taatcctgaa gccgtggtga aagtaaccga tgtattattg gcaagccatg aaaatacggc
    23221 gcaaattatt gatgcccgcc cggctgcacg ttttaacgca gaagttgatg aacctcgccc
    23281 aggtttacgt cgcggacata ttcccggagc actgaatgtt ccgtggacgg aactggtgcg
    23341 cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata ttttttggtc gcggcgtcag
    23401 ctacgacaaa ccaattatcg tcagctgcgg ctctggtgta acggcagccg tggttttgtt
    23461 agcactcgcg acgctggatg tgccaaacgt gaaactgtac gacggcgcat ggagtgaatg
    23521 gggcgcgcgg gcagatttac cggttgagcc agtgaaataa gtattttaca ggcaataaaa
    23581 aaccgccgaa tttggcggtt ttttattgct ggtctggttc gcggcctttc cagcaggttg
    23641 tattaccgta gtaatgcaag cgcgtctcag cggagacaat actcgccagt aactctcttt
    23701 ttgtcaagca aaagagagtt attattgttc tgttagtgta ttatccactg cggccctttc
    23761 cgccgtctcg caagcgggcg ctggcttaag gaaaggatgt tccgcgaccg ttagagcaag
    23821 cgcgtctcag cgcttgctat cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt
    23881 cgcatggacc gcacaatgaa gatacggtgc ttctgtatcg tacttattgt ttctggtgcg
    23941 ctgttttccc aagtaaataa taaccggagt ctctccggcg acaatttact ggtggttaac
    24001 aaccttcaga gcagcaagta agcccgaatg ccgcccttta gggcggcata ttttagatta
    24061 tccggttttg tttaaagtca cgcaaaaaac caccccagcg acgttcatag aatggcacaa
    24121 tatgttcggt aataaagtgg ctaattcctt tttccccttt tttcacctga caaatatcga
    24181 ttggttcatc gccaggtaat gtatcggtcg ctacacttcc cgtcgcctga ataatttctt
    24241 cgatatcacc atcggcttca atgccaataa gtaaattagg ctgtgcctct tcgttctctt
    24301 taattgaaca aataaaagca cgcttcaccg gcttaatggt tttaaataag gtggtgagtg
    24361 aatcaatcat ttgtgctggc ggctctgcga cttccgataa tatcagcgat tcaccgcctt
    24421 ccaggatttc ctggctgctc agcggatttc cctcttcacc aatcaacaaa ctgatttcac
    24481 gcggcataaa ttctttaccg gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg
    24541 tcatctcaaa caatgtgcga acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct
    24601 gttgaagcgc ttctaacgag gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc
    24661 agtgctgtaa atcaagcgca ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag
    24721 gcacccagac ggtggattcc agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg
    24781 ttgctgcttt ttccagcagg tcttcaagtt cgttttttgt ttcggacata agaaccacaa
    24841 ttcattcaac gttcggggcg caaatgcctg atgcgctacg cttatcaggc ctacaaggga
    24901 ttcgcaattt gttgaatttg cagaatttgt aggccggata aggcgtttac gccgcatccg
    24961 gcatattagt ttacgccgtt aacagattag ctatcgtgcg cacaccaagt cccgtagcgc
    25021 ccgcagacca ctgttcaacc ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc
    25081 agccttgctg atagttctca acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg
    25141 ccggatacgc cgcgcttccg gtattgttca gttcggcaaa gttagacggc agctggctgc
    25201 ggtggaattc cgccagcggc agacgccaga atggttcgtt ctcttgtgag gcactcgcca
    25261 gcaaacgacc ggcaagcgca tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg
    25321 cagttttcgc cgccccggtg agggtcgccg catcaatgat catttccggt ttctgcgcac
    25381 tggcatcaat cagaccatcg gcaagcacca gacgcccttc ggcatcagtg ttcatcactt
    25441 caactttttt accgttgcga taggtgatga tatcgcccag cttgaacgca ttgccgctaa
    25501 tcaggttatc cgcacagcag aggaacagct tcacgcgctt gttcagtccg cgcgtaatgg
    25561 caaatgccag cgccccggta accgttgccg cgccgcccat gtccgacttc atcgagtcca
    25621 taaacgcagt ctgtttgatg ctgtagccgc cggagtcaaa agtgatacct ttacctacca
    25681 ggcacgcgta cactggcgct tctttatcgc cagttgggtt gtaatccagc gccagcaata
    25741 ccggagaacg ttctgaaccg cgtccgacgg tgtgcagccc catataacct tgctcacgca
    25801 gatcttcgcc tttggtgatc cgataagtca cacgatcgcc cgcgacgttg ctgatcagat
    25861 caacagcacg ctgtgccagt tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt
    25921 cacgcaccca gtcgatgatc atcaggcggt tatccagttc ctggcgctgg gcatcgtcca
    25981 gatccggcca ctccacttta cgcgtgcctt tcggggcttt gtaaccttgc cagaatgccc
    26041 agcagcgatc cgcatcccag ccttcaccgc ttaactgaac atgcttgatg cccagaccgt
    26101 caatcttgcg cgccgcacgc tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg
    26161 taatgccgtc attattaatg ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt
    26221 gggtagagag ggtaatcttc atcgcttctg tcattttagt tatccttctt tgtaaaaggg
    26281 ccgccagttg gcagccccag gatttttccg ctaaatgatt cgcgttgcag aaaggcggca
    26341 agtgagtgaa gccccaggag catagataac tatgtgactg gggtgaacga gcgcagccaa
    26401 cgcatctgcg gcgtgaagca tgacgcggaa attattcggc ctcgtccagc cagactaaca
    26461 aaatcgcttc gagaattttc tcgttggatg cctgcgggtc gtcgtcgaaa tcttccagat
    26521 cgcaaatcca ctgatgcata tcggtgaatc gaaccgtttt cggatcaaga tcaggatacg
    26581 catcgtacag tgcttcgcca atttcgcggc tatcggtcca cttaagtccc atactaacct
    26641 ctgttaatgc tcacgcgcat ggttgatagt gtaacgcggg atttcgacta ctaaatcttc
    26701 gtcggtaaca cgtgcctggc agcttaaacg gctttcgggc tccagtcccc aggctttgtc
    26761 cagcatgtcg tcttcctgct ctgagctttc tggcagcgag tcaaaacctt cacgaacgat
    26821 gcagtggcag gtggtgcaag cacaggattt ttcacaggcg tgttcaatct cgataccgtt
    26881 acgcagagct gcgtcgagaa tggtttcacc gctattagct tccagaacag cgccatcagg
    26941 acagagatcc tgatgaggca aaataacaat ctttggcata ttaaacctcg tccacggaat
    27001 ggcctttcag cgcacgacga accgactggt ccatgcggcg agcggcgaaa tcctgggttt
    27061 gtttgtctac gtttttaatc gcttgttcga tggcgtcaac gtcatcgccc tgcgccactt
    27121 cactcaggtg agcggcagca tcgtcaatga cctgacgttc tgcggcgctt aacagcgcgg
    27181 catcagcagc cagcgcgccg tgcagacttt ccagcacacg cgccgcttct actttttgtt
    27241 ctgccagcat tcgggctttt acgtcctgct cggcatagct cattgagtct ttgatcatcg
    27301 aagcgatttc gctatcggtc agaccgtaag acggtttgac ctgaatagac gcctcaacgc
    27361 cggtggattt ctccatcgcc gtcacgctca aaagaccgtc ggcatcgacc tggaacgtca
    27421 cgcgaatatg cgcaccacca gccggtagcg ccggaatacc acgcagcgca aaacgcgcca
    27481 gtgagcggca gtcctgcacc agttcgcgct caccctgcat tacatggata gacatcgccg
    27541 tctgaccatc tttaaaggtg gtgaaatcct gagcgcgggc caccggaata gtggtattac
    27601 gcggaatcac tttctccacc aggccgccca tcgtttcgag gcccagcgac agtgggatca
    27661 catcaagcag cagcatttcg ctgtctggct tgttacccac cagaatatcc gcctgaatcg
    27721 ccgcgccaat agcgacgact ttatccgggt cgatggaagt cagcggtgga cgaccgaaaa
    27781 attcgcctac ccgttcacgc accagcggca cgcgagtaga accgcccacc atcaccactt
    27841 ccagcacttc gtcagcttct acacccgcgt ctttaagcgc gcgacgacaa gccagtaagg
    27901 ttcgttttac cagtggcgcg atcagttcat tgaattgttc acggctgatt tcgccctgcc
    27961 agcccgcaac gttaacggtc acggagtccg catcgctcag cgcgattttg gctgcaatgg
    28021 cggcatccag cagttcacgc tgaacgcggt tatcgctacg atcaggaatg cccgcctgct
    28081 cgcgaatgta atccgccagc agatggtcga aatcatcgcc gccgagcgcg gaatcaccgc
    28141 cggttgccag cacttcaaac acgccgcgac ttaagcgcag aatggaaata tcaaacgtcc
    28201 cgccaccgag gtcataaaca gcgatcacgc cttcctgacc ggaatccagc ccgtaggcga
    28261 tagccgcagc ggtcggttcg ttaagtaagc gcagaacatg caaccccgcc agacgcgccg
    28321 cgtctttggt gccctgacgc tgggcatcgt caaagtacgc cggaacggtg ataactacac
    28381 catccagctc gcctgccagg gcttcagttg cccgcgccgc cagtgctttg aggatgtccg
    28441 cagaaacgcg caccgggttc agcagccccg ccgccgtttc aatcatcggc aggccgtttt
    28501 cgctggcctg gaattgataa ggcagatgcg gatagcgttg ctggatatca gccagcgagc
    28561 gccccatcag gcgtttaaca gaactaatgg tgttggcggt gtcaagcgct gcgttagtac
    28621 gcgcgtcata acccaccgaa tgcccttgct gttgatagtg aacaacagat ggcagcaggt
    28681 gacggccttc atggtcggct aacgtttcgg cctgaccgct gcgcactgtc gccaccagcg
    28741 agttggttgt gcccaggtca ataccagccg ccagacgacg ctgatgcggc gcagcactca
    28801 aaccaggttc actaatttgt aataaggcca tgtttagctt ccagaaatta aaaatcgagc
    28861 agtttttctt cgagttgttc ggcactgctc cgcagtttat cgagaaaacg cagcttacgc
    28921 acggtatccg ccgccgcgtc ccacgcctcg ttgtctaact gttcaaccat caactgatgg
    28981 cgggtatcaa acatcttttt cacacgtttg ataaagcttt ccagccgcgc ttcatctttc
    29041 gcctgttcga tctcgtccag ctcttcgcgc agctccaact gttccatcag gaacgcggtg
    29101 tcgcgcacag tatgctgctc gctggcgaga tcaaaaccgt gtaaagaaag caaatattcc
    29161 gcgcgcatta acggatgacg cagcgtttgc caggcctggt taatggttgc agattgctgt
    29221 acggcggcga gttgttccgc ctggcttccg ctggcgaatt tatcaggatg atactgacgt
    29281 tgtagatcct gaaaacgcag gctcagcgcc tgggtatcga gttgataacg ggcaggcaag
    29341 ccaaagaagg tgaagtaatc cataacattc tcagggctgc gatccgccgc gataacggcg
    29401 aatcaggtaa aacaaacccc acgcgcaggc gaccacggtg gggttatcgg tatgcgcatc
    29461 aaacgtggaa gctttcgccg caaccacact catctttgac gttcgggttg gtgaatttaa
    29521 acccttcgtt caggccttct tttacgaagt ccagctgcgt accgtccaga aattgcaggc
    29581 ttttgccatc gaccacgact ttcacgcctt tgtcttcaaa cacgatgtct tccggcgtcg
    29641 gttcgtcaac aaattccagt acataagcca tacctgaaca tccggaggtt ctcacgccca
    29701 gacgcaggcc aaaccctttg ccgcggttag ccagaaaggt atttactcgc gctgctgcac
    29761 tgtcgctcag tgtaatcgac ataaccaaac ctcaactctt attttgcttc acgtttgctt
    29821 ttatagtccg caatggcggc tttgatcgcg tcttctgcca gaatagaaca gtgaattttc
    29881 accggcggca gttcaagttc ttcagcaata tcggtatttt tgatcgcctg cgcttcgtcg
    29941 agagacttcc ctttcaccca ttcggtgacc agggagctgg aagcgatagc agaaccgcag
    30001 ccgtaagttt taaaacgcgc gtcttcaatg ataccttcat cgttgacttt aatctgcaac
    30061 ttcatcacgt cgccacaggc cggtgccccc accatgccgc tgccgacgtt ctcgtcgttg
    30121 ttgtcaaagg aacccacgtt acgcggattc tcgtaatggt cgataacttt ttcgctgtaa
    30181 gccattataa attctcctga ttccgatacc gattaatgat gagcccattc gatgctgttc
    30241 agatccacgc cctgcttgta catttcccac agcggagaaa ggtcacgcag acgaccgatg
    30301 gatttacgaa ctaactcgat ggtgtagtcg atctcttctt cagtagtaaa acgacctaaa
    30361 gagaaacgga tagagctatg tgccagctcg tcgttcagcc ccagcgcgcg cagcacgtag
    30421 gacggttcga ggcttgctga cgtacaggcg gaacctgaag aaactgcgag gtctttcagc
    30481 gccataatca gcgactcacc ttcaacgtag ttgaagctga cgttgagaat gttcggcgca
    30541 ccgtgttcca ggtcaccgtt caggtaaact tcttcgatat ctttgatgcc gttccacaga
    30601 cggttacgca ggccgcgcag acgttccatc tcggtcgcca tctcttcttt tgcgatgcga
    30661 taggcctcgc ccattccgac gatctggtga acaggcagag tgccggaacg cataccgcgc
    30721 tcgtgaccgc cgccgtgcat ttgcgcttcg atgcgtacgc gcggtttacg acgtacatac
    30781 agcgcaccga tacctttcgg gccatagatt ttgtgaccgg agaaagacat caggtcaact
    30841 ttcaactggc tcaggtcgat aggcagtttg cccacgctct gggttgcatc aacgtgatag
    30901 ataatgccac gagcacggca catttcgccg atagccgcga tatcctgcac cacgccgatt
    30961 tcgttattta cgtgcatgat ggaaacaagg atggtgtcat cacgcatcgc tgcttcaagt
    31021 tctttcagat cgataatgcc gttacgctgc ggtgccaggt aggtgacttc aaaaccttcg
    31081 cgctccagct gacggcaggt atccagtacc gctttgtgtt cggttttgct ggtgatgatg
    31141 tgcttgcctt ttttctgata aaagttggct gcacctttga tcgccaggtt gtcagattcg
    31201 gttgcaccag aggtaaagac gatttcacgc ggatcagcgc cgaccagatc ggcaatctga
    31261 ttacgggcga tatctaccgc ttcttcagcc tgccagccga aacggtgaga acgggaggcc
    31321 gggttaccaa aggttccgtc catcgtcata aactgcatca ttttctcggc aacacgcggg
    31381 tccaccggcg tggttgcgga gtagtcgaga taaatcggta atttcattgc tctataaact
    31441 ccgtacatca ctcaatgcaa ggaaccaggc taccggctgg atgtacgacc gtgtttacgg
    31501 agcgcgatac gccccggcct gattctaata tcttgttatc tttaacctaa ctgtttcggg
    31561 tttcagcaag ttgacaaact tacgaatctc cgggagctta cataagtaag tgaccggagt
    31621 gagtatgtgc agtcaacgca gatgcaacac gaaacaggac ggttaaatta agcgcgtaac
    31681 ttaacgtcga tcgcgtcttg tatgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca
    31741 gacacatcca gcacttcctg gttattaacc agttcgccta aagtaatgtt gttgagaaaa
    31801 ccggtgagac ggtcgctcaa atcacgccac agcgcgtggg tcaggcattt atcgccgccc
    31861 tggcaaccgc ctttaccctg acaacgggta gcatctacag attcgtcaac ggcgctaatc
    31921 acttcgccaa cggcgatgct gctggcatct ttgcctaaca gataaccacc gcctggtcca
    31981 cgtacgctgg aaaccagacc atttttacgc agacgggaaa acagttgttc cagataagaa
    32041 agggaaattc cctgacgttc ggaaatatca gccaacggta ccgggcccgc ttcagagttg
    32101 agcgcaacgt caagcattgc ggtcacggca tagcgccctt tagatgtcag tctcatgtct
    32161 tacttcacct caaactcgcc cctgcccggg gttttttatt gtaaaagtgg gggtattgca
    32221 tagcagggtc aagtctgaca ttcccgagta aattggtcaa ctatttactt gactgattta
    32281 gtcgggtatt taaccttcag tgccattttt ttatcgtggc gtttgtacct gttgtccttc
    32341 cagaccatgg gcgggttgag ggcaattatt cgcattagcg ctggcctctt cccctcaccc
    32401 taaccctctc cccgaagggg cgaggggacc gtccgtgcat ttatctggct tagccctcag
    32461 gccttattac ctttattctg ctgctctata gaagccagaa tcccgcgcag gatattcaat
    32521 tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat cacctgcccc
    32581 ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg accataaaaa
    32641 cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg ctcgccattt
    32701 tcctgagttg ccagccaggc catacgcact tcataggcaa taacctgaac cgccattgcc
    32761 aagttcagcg aactgtattc cgggtttgcc gcaatcgcga catgataatg gcatttctgc
    32821 aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac cggggtattt
    32881 gccgcttcag cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg ccacggcagc
    32941 gtgcgggagc gtgcgctggt gcccaccacc aggctacaac cggctaacgc ttcgtcgagc
    33001 gtatcgacga tgtgagcatt accaatcaca tcgctggccc ctgctgccag ggcaatcgcc
    33061 tgggagtcgg gtttcaccag tggattaacc agccacagat tggttaatcc cattgttttc
    33121 atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag cacaattcga
    33181 atattttgca gcattgtctt tcttcatcta aagattattc acgcatctta tcataaaacg
    33241 aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt taacatccag
    33301 tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg caaggcgggt
    33361 aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca gaaaggcagt
    33421 aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga cacgattcgt
    33481 aaatcttact cacagcacac catcatcacc gaagaaagcg gtgaacttga aggtactgat
    33541 caggatgttc aatgggttat cgatccactg gatggcacta ccaactttat caaacgtctg
    33601 ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt tgctgtggta
    33661 tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcagggcgc acagctgaac
    33721 ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg gtaccattct ggcgaccggc
    33781 ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg caaactgttc
    33841 aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc ttacgtcgct
    33901 gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt cgcggcaggc
    33961 gagctgctgg ttcgtgaagc aggcggcatc gtcagcgact tcaccggtgg tcataactac
    34021 atgctgaccg gtaacatcgt tgctggtaac ccgcgcgttg tgaaagccat gctggcgaac
    34081 atgcgtgacg agttaagcga cgctctgaag cgttaatgac tcaggcgggt gatgtcactc
    34141 acccgccctc gcctttcagg cgctattctg aaatacttcc tcaccgcttt actttctttc
    34201 gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcaca aggaaaggtt
    34261 atggcgctgc cagtgaacaa acgcgttctc aaaattctgt ttattctctt tgttgttgcc
    34321 ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc tgacgataaa
    34381 atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa ggacggtact
    34441 cgtctgcaag gctggtttat tccttcttcg acgggccctg ctgacaatgc catcgcaacc
    34501 atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct ggtcagttgg
    34561 ttacccgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg taaatcaaaa
    34621 ggcacgccgt cccaggccgg attgctggac gatacgcaaa gtgccatcaa tgtggtgcgc
    34681 catcgcagtg atgtaaaccc acaacggctg gtgctgttcg ggcagagtat tggcggggcg
    34741 aatattcttg ctgttattgg tcaggatgat cgtgaaggca tacgtgcggt gatcctcgac
    34801 tccacatttg cctcttatgc aaccatcgcc aatcagatga tccccggcag tggctactta
    34861 cttgatgaga gttacagcgg cgaaaattat atcgccagcg tcagcccgat cccgctttta
    34921 ctcattcacg gtaaagctga tcacgttatc ccatggcagc acagcgaaaa gttgtatagc
    34981 ctggcaaaag agacaaaacg gttgatccta atcccggatg gcgaacacat tgatgctttt
    35041 tccgatcgtc acggcgatgt ttatcgcgaa cagatggtgg actttatcct tagcgcgttg
    35101 aatccgcaga actaacccat gatcgctagc acgataatca ttcacaaaac caccttaaga
    35161 catgctaatc cactggtcag aacagtttaa gatgagaaaa attctgtgac gcttgccaac
    35221 atttctgatg attagcattc ccttcgccat ttccctgagc aaactttagc tattcttatc
    35281 aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat ctgtcctttc
    35341 ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt tgaccgccac
    35401 catggcaacc ttcagcgagc ttaatggtgt ggatgatgat attgccagtc ttgatatcag
    35461 cgaaacagga cgggagatcc tgcgctatca tcaactcaca ctgacgactg gttatgacgg
    35521 tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc actggctacg
    35581 acgtggtttc cgtctgtgtc cgtcatttat taccagccaa ttcacccccg ccctgaagag
    35641 tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc tacaagcgtt
    35701 agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa acgcgcgata ttcatttcct
    35761 gtgtctgtat ctgcaatatt gtttgctgca acaccacgct ggaattacgc ctcagtttaa
    35821 tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc aggaaattgg
    35881 acgccactgg cagcgtcggg cgctccagcc tgtaccgcct gatgagccac tgtttatggc
    35941 attacttttt tccatgttgc gggttcccga tccattgcgt gatgcgcatc agcgagacag
    36001 acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg gaaatgttcg
    36061 tttttatgat gaacaggggt tatgcgatca gctttatacc cacctcgccc aggcgttaaa
    36121 tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg ccagactgta
    36181 cccacgcctg gtgcgcacca cccgcgcggc gctggccgga tttgaaagtg aatacggcgt
    36241 ccatctttct gatgaggaaa gcggtctggt cgcggtgatt ttcggtgcct ggctaatgca
    36301 ggaaaacgat ctgcatgaaa aacagattat tctactgacc gggaatgata gcgagcgaga
    36361 agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca ttaagcatat
    36421 gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga ttattgcgcc
    36481 ttataccatg ccattaccgc tcttttcacc accgctgatc tatacggacc tgacgctgac
    36541 aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga acaactttcg
    36601 ggcgcaaaaa cattgccgga agcgccacca gcgccattac ccagaacacg ccgtggccca
    36661 gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg cccatcgcga
    36721 cggcagagta aaccgcctgt aaacggatga cttcgctacc ctggcgaaca gcaatatagc
    36781 gcatggcggc caggtggcag accgtgaagg tgccgcaatg caaaatttgc accactatca
    36841 accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg caaatcgccg
    36901 agatcaacag catatcgcga gcactacaac ggcggaaaag tttattactc agcgcaaaga
    36961 taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag gccgagtagc
    37021 cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc ccctgcaata
    37081 aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac gcagaccaac
    37141 cggtgctctc ctgctggcga cttgcccctt gtggctgaat cgtcggacgg atgagaaagc
    37201 cgagcagcat ggatgccacg cccaacgtca acagcgcgag gatcacccga taatcaaaca
    37261 tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag ccccacagtc
    37321 gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt gcatcggtca
    37381 acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc agccacgcta
    37441 cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgt cagcagtgcc agcacgcgca
    37501 aggcggaaat caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa ctcccgagaa
    37561 aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt aaaccaatcc
    37621 ctttaagcca gacgctccag aaaggtagaa aaatgccgta actaaaaaag tatgtgaaat
    37681 agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt caggaggcgt
    37741 tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc gagcctgctt
    37801 cccgatgtct gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg acgcatgttg
    37861 caaaaataat gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca atggcgatga
    37921 aatcgcgtaa tgccggcttc atctcccctt tcttccacgc catcagtaaa gcaatggaag
    37981 gcacatttcc ggcaatggga cgaaaaacaa cctgcccggt attaaaatta ttcatataac
    38041 cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc agaatattcg
    38101 ttgccacctg gacgatattt ggctggctgt tttcttgcgc aaaccacgct ttaacgatcg
    38161 gcgcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca tccagttgcg
    38221 cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacacc accactaatg
    38281 gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg cgcattaagc
    38341 cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt aaactcacca
    38401 gctcaatcaa ggtgtctggc tgtctgagac gaaacattgg taatactttt ggcaataaat
    38461 tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga acaattttcc
    38521 gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc tggagaaaac
    38581 attctcccgc cgccgtcaac gcgactttgc gcttatccct caccagtaac ggaacaccga
    38641 cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta tgcagttttt
    38701 ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt aaatgccgta
    38761 gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa ttaactattt
    38821 cacatattag caaccaacca acaacatcct tatgacacaa aaatagaagg tcaatacatc
    38881 ttatctttca ggattaaaaa atatgaccac accctcagat ttaaacattt accaactgat
    38941 tgatacccaa aatggtcggg tcactccgcg tatttatacc gacccagata tttaccaact
    39001 ggagcttgaa cgtattttcg gccgttgctg gttatttctc gcccacgaaa gccagatccc
    39061 aaaacctggt gatttcttta acacctacat gggggaagat gcggttgtcg tagtgcgtca
    39121 gaaagacggc agcattaagg cgtttctcaa ccaatgccgc caccgggcca tgcgtgtgag
    39181 ttatgcagat tgcggcaaca ctcgcgcctt tacctgtccg tatcacggct ggtcttatgg
    39241 cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac aagggttgtg
    39301 taaatcccac tggggactaa acgaagttcc ttgtgtggag agttataaag ggctgatttt
    39361 tggcaactgg gataccagcg caccgggcct gcgtgattac ctgggtgaca ttgcctggta
    39421 tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg gcgtacaaaa
    39481 gtgggtgatc aactgtaact ggaaattccc ggcagagcag ttcgccagtg accagtatca
    39541 tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg atggcagcga
    39601 taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca aagatgcgct
    39661 gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac cggatgctaa
    39721 tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg aagcagaaca
    39781 acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatattt tccccacgct
    39841 ttcatggctc aacggcactg ccacgctccg cgtctggcat ccgcgcggcc ctgatcaagt
    39901 tgaagtgtgg gcgttctgta ttactgacaa agccgcctcc gatgaagtta aagccgcttt
    39961 tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg atgactcgga
    40021 gaactggtgt gaaatccaga aattgcttaa aggccaccgc gcccgcaaca gcaaactgtg
    40081 tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg gcattactaa
    40141 ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg atcttctgag
    40201 tagcgaaagc tggcaggaag tgctcgataa aaccgccgct taccagcagg aggtgatgaa
    40261 atgagtgcgc aagtttcact agagttacat caccgcatta gccagtttct ctttcacgaa
    40321 gccagcttac tggacgactg gaaatttcgt gactggctgg agcagctcga caaagagatt
    40381 cgttacacca tgcgcaccac agttaacgcg caaacacgcg accgccgcaa aggcgtccag
    40441 ccaccgacaa cctggatttt taatgacacc aaagaccagc tggagcggcg aatcgcccgt
    40501 ctggaaacgg gcatggcctg ggcagaagag ccgccgtcac gcacccgtca cttaatcagc
    40561 aactgccaga taagcgaaac cgacatccca aacgtatttg ctgtgcgggt aaattatctg
    40621 ctttatcggg cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg tttcgacaaa
    40681 gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt cctggatcaa
    40741 gcggtaatca cttcccataa cctgagtgta ctgttctgat gaatcgaatt tatgcgtgtc
    40801 ccgttgcgga tgtgccggag ggtgaggctc tccggatcga tacctcgccc gtcatcgccc
    40861 tgttcaacgt tggcggcgag ttttatgcca ttaacgatcg ttgcagccat ggtaatgcgt
    40921 cgatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta cacgccgcca
    40981 gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg ctcaccactt
    41041 atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg cagccatgag
    41101 cgatctgcat aacgagtcca tttttattac cggcggcgga tcgggattag ggctggcgct
    41161 ggtcgagcga tttatcgaag aaggcgcgca agttgccacg ctggaactgt cggcggcaaa
    41221 agtcgccagt ctgcgtcagc gatttggcga acatattctg gcggtggaag gtaacgtgac
    41281 ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg gcaagctgga
    41341 ttgttttatc ggcaatgcag gcatctggga tcacaatgcc tcactggtta atactcccgc
    41401 agagacgctc gaaaccggct tccacgagct gtttaacgtc aacgtactcg gttacctgct
    41461 gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga ttttcacact
    41521 gtcaaatgcc gccaggtatc ctggcggcgg tggcccgctg tacaccgcca gtaaacatgc
    41581 cgcaaccgga cttattcgcc aactggctta tgaactggca ccgaaagtgc gggtgaatgg
    41641 cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg ggcaaagtga
    41701 aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac cgctgcaatt
    41761 tttcccgcaa ccggcggatt ttacggggcc gtatgtgatg ttggcatcgc ggcgcaataa
    41821 tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc gcggcattcg
    41881 ccacgtagcg gctgggctgg atctttaagg aagcacgatg aaagaaaaaa cgatcattat
    41941 tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc aagggttcac
    42001 cggtgagctg catctgtttt ccgatgagcg acatcttcct tatgaacgac ctccactctc
    42061 gaaatccatg ttgctggaag attccccgca gttacagcag gtgttacccg ctaactggtg
    42121 gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg gtcgcgacac
    42181 acgagagtta gtgttaacca acggcgaaag ctggcactgg gatcagcttt ttatagcaat
    42241 cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct gctttaccct
    42301 acgccatgcc ggtgatgccg ccagactgcg agaagttctg cagcccgaac ggtcagtcgt
    42361 gattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc agcgcagatg
    42421 taaggtgaca gtgattgaac tggcggcaac cgtcatgggc cgtaatgcac caccgcccgt
    42481 gcaacgctat cttttacagc gccaccagca ggctggtgtg cgcattctgc tcaataatgc
    42541 cattgaacat gtggtcgatg gtgaaaaagt agaactgacg ctgcaaagtg gggaaacgct
    42601 tcaggctgat gtggtgattt acggtattgg tatcagcgcc aacgagcaac tggctcgcga
    42661 ggccaacctt gatactgcca atggcattgt cattgatgag gcttgccgca cctgcgatcc
    42721 cgcgatcttt gccggtggcg atgtggcaat cactcgtctt gataatggtg cactacaccg
    42781 ctgcgaaagc tgggaaaacg ccaataacca ggcgcaaatt gccgctgccg caatgttagg
    42841 gctaccgcta ccgctactgc cgccgccgtg gttctggagc gatcagtaca gtgataactt
    42901 acagtttatt ggcgatatgc gtggcgatga ctggctttgt cgtggcaacc cggaaactca
    42961 gaaggcgatt tggtttaatc tgcaaaacgg cgtgcttatc ggtgcggtca cgctgaatca
    43021 ggggcgtgag attcgcccaa ttcgcaaatg gatccagagc ggcaaaacgt ttgatgcgaa
    43081 actgctgata gatgagaaca tcgcgcttaa atcactgtaa ccaggataat tagcgaatat
    43141 ctcaacgcct ggggcgtggc gaggtgcaag agtgtgtatt acgtttaaat cacattatct
    43201 tgcaaaggga ttggttatga acacacta
//
LOCUS       DAZPAC010000009        42130 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZPAC010000009 DAZPAC010000000
VERSION     DAZPAC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.009"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..95)
                     /locus_tag="ACHUPM_000400"
     CDS             complement(<1..95)
                     /locus_tag="ACHUPM_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HFR0868286.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWL"
     gene            complement(88..576)
                     /gene="hybE"
                     /locus_tag="ACHUPM_000401"
     CDS             complement(88..576)
                     /gene="hybE"
                     /locus_tag="ACHUPM_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR0868287.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(569..1063)
                     /locus_tag="ACHUPM_000402"
     CDS             complement(569..1063)
                     /locus_tag="ACHUPM_000402"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR0868288.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1063..2766)
                     /gene="hybC"
                     /locus_tag="ACHUPM_000403"
     CDS             complement(1063..2766)
                     /gene="hybC"
                     /locus_tag="ACHUPM_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR0868289.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(2763..3941)
                     /gene="hybB"
                     /locus_tag="ACHUPM_000404"
     CDS             complement(2763..3941)
                     /gene="hybB"
                     /locus_tag="ACHUPM_000404"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR0868290.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(3931..4917)
                     /gene="hybA"
                     /locus_tag="ACHUPM_000405"
     CDS             complement(3931..4917)
                     /gene="hybA"
                     /locus_tag="ACHUPM_000405"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR0868291.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(4920..6038)
                     /gene="hybO"
                     /locus_tag="ACHUPM_000406"
     CDS             complement(4920..6038)
                     /gene="hybO"
                     /locus_tag="ACHUPM_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR0868292.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6227..6514)
                     /gene="yghW"
                     /locus_tag="ACHUPM_000407"
     CDS             complement(6227..6514)
                     /gene="yghW"
                     /locus_tag="ACHUPM_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR0868293.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(6633..7520)
                     /gene="yghX"
                     /locus_tag="ACHUPM_000408"
     CDS             complement(6633..7520)
                     /gene="yghX"
                     /locus_tag="ACHUPM_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR0868294.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            7677..8717
                     /gene="gpr"
                     /locus_tag="ACHUPM_000409"
     CDS             7677..8717
                     /gene="gpr"
                     /locus_tag="ACHUPM_000409"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR0868295.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(8757..9251)
                     /locus_tag="ACHUPM_000410"
     CDS             complement(8757..9251)
                     /locus_tag="ACHUPM_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR0868296.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9442..10326
                     /gene="yghA"
                     /locus_tag="ACHUPM_000411"
     CDS             9442..10326
                     /gene="yghA"
                     /locus_tag="ACHUPM_000411"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0868297.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(10486..10911)
                     /gene="exbD"
                     /locus_tag="ACHUPM_000412"
     CDS             complement(10486..10911)
                     /gene="exbD"
                     /locus_tag="ACHUPM_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR0868298.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(10918..11652)
                     /gene="exbB"
                     /locus_tag="ACHUPM_000413"
     CDS             complement(10918..11652)
                     /gene="exbB"
                     /locus_tag="ACHUPM_000413"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0868299.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            11904..13091
                     /gene="metC"
                     /locus_tag="ACHUPM_000414"
     CDS             11904..13091
                     /gene="metC"
                     /locus_tag="ACHUPM_000414"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR0868300.1"
                     /translation="MTDKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13231..13890
                     /gene="yghB"
                     /locus_tag="ACHUPM_000415"
     CDS             13231..13890
                     /gene="yghB"
                     /locus_tag="ACHUPM_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR0868301.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(13930..14829)
                     /gene="yqhC"
                     /locus_tag="ACHUPM_000416"
     CDS             complement(13930..14829)
                     /gene="yqhC"
                     /locus_tag="ACHUPM_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR0868302.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15023..16186
                     /gene="yqhD"
                     /locus_tag="ACHUPM_000417"
     CDS             15023..16186
                     /gene="yqhD"
                     /locus_tag="ACHUPM_000417"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR0868303.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16291..17118
                     /gene="dkgA"
                     /locus_tag="ACHUPM_000418"
     CDS             16291..17118
                     /gene="dkgA"
                     /locus_tag="ACHUPM_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR0868304.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17318..18244
                     /locus_tag="ACHUPM_000419"
     CDS             17318..18244
                     /locus_tag="ACHUPM_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR0868305.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18295..18552
                     /gene="yqhH"
                     /locus_tag="ACHUPM_000420"
     CDS             18295..18552
                     /gene="yqhH"
                     /locus_tag="ACHUPM_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR0868306.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(18595..20814)
                     /locus_tag="ACHUPM_000421"
     CDS             complement(18595..20814)
                     /locus_tag="ACHUPM_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR0868307.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(20925..22337)
                     /gene="ftsP"
                     /locus_tag="ACHUPM_000422"
     CDS             complement(20925..22337)
                     /gene="ftsP"
                     /locus_tag="ACHUPM_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR0868308.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(22412..23149)
                     /gene="plsC"
                     /locus_tag="ACHUPM_000423"
                     /gene_synonym="parF"
     CDS             complement(22412..23149)
                     /gene="plsC"
                     /locus_tag="ACHUPM_000423"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR0868309.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(23382..25640)
                     /gene="parC"
                     /locus_tag="ACHUPM_000424"
     CDS             complement(23382..25640)
                     /gene="parC"
                     /locus_tag="ACHUPM_000424"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR0868310.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(26186..26668)
                     /locus_tag="ACHUPM_000425"
     CDS             complement(26186..26668)
                     /locus_tag="ACHUPM_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR0868311.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(26721..27113)
                     /gene="ygiW"
                     /locus_tag="ACHUPM_000426"
     CDS             complement(26721..27113)
                     /gene="ygiW"
                     /locus_tag="ACHUPM_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR0868312.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27265..27924
                     /gene="qseB"
                     /locus_tag="ACHUPM_000427"
     CDS             27265..27924
                     /gene="qseB"
                     /locus_tag="ACHUPM_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR0868313.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            27921..29270
                     /gene="qseC"
                     /locus_tag="ACHUPM_000428"
     CDS             27921..29270
                     /gene="qseC"
                     /locus_tag="ACHUPM_000428"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR0868314.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            29380..29961
                     /gene="mdaB"
                     /locus_tag="ACHUPM_000429"
     CDS             29380..29961
                     /gene="mdaB"
                     /locus_tag="ACHUPM_000429"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR0868315.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            29992..30306
                     /locus_tag="ACHUPM_000430"
     CDS             29992..30306
                     /locus_tag="ACHUPM_000430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR0868316.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(30354..32246)
                     /gene="parE"
                     /locus_tag="ACHUPM_000431"
     CDS             complement(30354..32246)
                     /gene="parE"
                     /locus_tag="ACHUPM_000431"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR0868317.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(32275..32856)
                     /gene="yqiA"
                     /locus_tag="ACHUPM_000432"
     CDS             complement(32275..32856)
                     /gene="yqiA"
                     /locus_tag="ACHUPM_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR0868318.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(32856..33683)
                     /gene="cpdA"
                     /locus_tag="ACHUPM_000433"
     CDS             complement(32856..33683)
                     /gene="cpdA"
                     /locus_tag="ACHUPM_000433"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR0868319.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(33708..34130)
                     /locus_tag="ACHUPM_000434"
     CDS             complement(33708..34130)
                     /locus_tag="ACHUPM_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR0868320.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(34131..34760)
                     /gene="nudF"
                     /locus_tag="ACHUPM_000435"
     CDS             complement(34131..34760)
                     /gene="nudF"
                     /locus_tag="ACHUPM_000435"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR0868321.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            34965..36446
                     /gene="tolC"
                     /locus_tag="ACHUPM_000436"
     CDS             34965..36446
                     /gene="tolC"
                     /locus_tag="ACHUPM_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR0868322.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            36594..37265
                     /locus_tag="ACHUPM_000437"
     CDS             36594..37265
                     /locus_tag="ACHUPM_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR0868323.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            37271..38431
                     /locus_tag="ACHUPM_000438"
     CDS             37271..38431
                     /locus_tag="ACHUPM_000438"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0868324.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(38469..39257)
                     /gene="ygiD"
                     /locus_tag="ACHUPM_000439"
     CDS             complement(38469..39257)
                     /gene="ygiD"
                     /locus_tag="ACHUPM_000439"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR0868325.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            39400..40173
                     /gene="zupT"
                     /locus_tag="ACHUPM_000440"
     CDS             39400..40173
                     /gene="zupT"
                     /locus_tag="ACHUPM_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR0868326.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(40231..40401)
                     /gene="yqiD"
                     /locus_tag="ACHUPM_000441"
     CDS             complement(40231..40401)
                     /gene="yqiD"
                     /locus_tag="ACHUPM_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR0868327.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(40662..41315)
                     /gene="ribB"
                     /locus_tag="ACHUPM_000442"
     CDS             complement(40662..41315)
                     /gene="ribB"
                     /locus_tag="ACHUPM_000442"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR0868328.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(41419..41567)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            41689..41979
                     /gene="ubiK"
                     /locus_tag="ACHUPM_000443"
     CDS             41689..41979
                     /gene="ubiK"
                     /locus_tag="ACHUPM_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR0868329.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT        10446 a        11267 c        10572 g         9845 t
ORIGIN      
        1 tccagccaca cggcggtgac gcgcttaaca tcgtgctgct ccgcctgccg ttggataatt
       61 tcaacggcgc tctggcaaag agacaactca tgcattttcg ccactcctgc gaccaaacag
      121 cagggcgcga cgccctgcgt gtggtacatc cggattcgtg actggcagcg aaaggatcat
      181 tcgtgcgcag tcatccgtca ggcgttggcc ctcttcaatc gacatgctgt gcgaaagcgg
      241 cgacatcagc gaacaggaga gatattgcga aacaccgtcc agttcgccaa cggtaaaggt
      301 catagtgcca tacggcagtt gcagaccaat tttttcactc actttgcgca gcggccagag
      361 ttgatccggg ccggggaaga taactgcact cagcatccac ggggtgatca cacaccccgt
      421 ccactgacct tcgaacagcg taaaatcaga aacatacacc ggcattgaag gatgcagaaa
      481 agagagatcg tgcatcgaac gccgggcaat ttcttcaaac gctgcctgta cttgcgcctt
      541 cggggaggtc tggaaacctg ctatctcttc agtcatgaat cgcctcccgt gggatggctt
      601 ccacgcctga ttcgcgcagc gcagccagaa cctgctcaag cgcaggttca atcattgctt
      661 caaccgtcgg cgttaagccg atgtgtggct ccagcgattc cgggatcacg ccgaccaggg
      721 tcagcttttt cggaaactca ccggtgaagc gcagggccga caatacgtcg gccaggccaa
      781 gctgatgcgg agagattttg ttggtaaaca acgccggaac ttcttcatcc cgcaggatca
      841 tcatcgttcc cggcgtgttc tttttcgaca caatggcatc cgcaataatc agatgatcgc
      901 gatttgccat gtcgccaagc agctccattc ccgccgtgcc gccatcgagg atctcaacat
      961 aatccggcag aatgtatcgt tgctctaacg cttcgacaat ccgcacaccg atggcttcat
     1021 cggtcagcaa aatattgccg acccctaaga ctaaaatacg cattacagaa ccttcactga
     1081 aaccacttcg ttgccgtcgg catccactac gtgtaccgca caggccatgc acgggtcgaa
     1141 ggagtgaatg gtacgcacca cttccagcgg tttattcgga tcagcaaccg gtgtacccac
     1201 cagcgactgc tcgtaaggac cgacgtcatc attgaagtta cgcggaccag agttccaggt
     1261 tgatggaaca actgcctggt agttactgat aataccgtct ttaataacca tccagtgaga
     1321 gagcataccg cgcggcgctt cgaggaagcc aacacctttg aactcacccg ttgccggaat
     1381 gttcggtttc acaaaggtgg tgtgatcgcc tttgccgata ttggtgatca gtgcactgta
     1441 ttgattttgc aggatatcct gcaattcgca gcagtgaacg gtacgaccaa taatacggcc
     1501 cagcgtggag tgcagctgcg ccacttccag cgtgttgcca gtcagtttct gataaatcgc
     1561 aacgatttca ttcagtttgt tctgggtaga ttcgcgacct gccgccagtt tcaccagcat
     1621 attagccagc ggacccactt ctaccgtttt gccgtagaaa gtcggtgatt tcacccagga
     1681 atatttcccg tcgtcagacc aaccatcata agccggaatg gtggtacctt cccacggtgc
     1741 ctgcggcgct tcgtctttat accaggagtg cttcgcgctt tcctgaatgc ctttgatcag
     1801 gtattcatcg gaatgagaag tgatcggacg atacgaggac agatccgcat tctcaatgta
     1861 gccgcccggg aacaggaagc tgccgttttt actgtcggtc gggaattccg gcacgctcag
     1921 gtagttcacc gcacctttac cgcgcgtcag ccattccggg tagaacgcgg caataactgc
     1981 ggtatcaacc ttataaacct gttcaacaaa gtcgctcagc ttgtcgatga aagacttgat
     2041 gtacatcagg cgctcaaggt tcagcacgcc caaaccgtcg aggttgatcg ggttcgcgac
     2101 accacctacc gccaggttct gaatgtgcgg cgttttaccg cccagcagcg ccacgacgcg
     2161 gttagcgtca cgctggcact ccagcgcctg caggtagtgc gctaccgcaa tcaggttcac
     2221 ttccggcggc agtttcatcg ccgggtgacc ccagtagcca ttggcgaaaa tacccaactg
     2281 accgctggca accagatctt tgatcttgtt ctgaacttta gtgaactctt ccggactgtt
     2341 cagatgccag gtcgaaacgc ctttcagcat ttcggaggct ttggttgggt cagcttgcag
     2401 tgcagaagtg atgtccaccc agtccagcgc cgaaagctga tagaaatgaa cgatatggtc
     2461 atgcgtggtg tgcgcagcca gaatgatgtt acggatgtat tgcgcgttaa ccggaacgtc
     2521 gatattcagc gcactttctg ccgcacgaac ggaagacagc gcgtgagtgg tagtacatac
     2581 gccacagata cgttgcacaa tcatccatgc atcgcgcgga tcgcggtttt tcacgatctc
     2641 ttccatgccg cgccacatgg taccggaagc ccatgctttc gaaacgacgc cattttcgat
     2701 ttcgcaatcg atgcgtaaat gcccctcaat acgggttacc ggatcaatag taattctctg
     2761 gctcatgctt tgctcgcctc atgacgatta tgatcgtttt gttttaaagg aggaagtatc
     2821 ggcagtagac gaatgagtac gatgtaagcg caaatctcaa tagccacaaa accaatagaa
     2881 atcaacagtt cttcccaggt cgggaagtag gcgtaaccgc cgcccgggtt gaatgccacc
     2941 agcgaatagg tcagacgcca ggttgcacaa cctaacagtg cgctcagtgc tgacaggaac
     3001 agcatgcggg aatcattacg cagcttcgcc acacgcagaa cgaccagcgg gaagagcatc
     3061 agcaggactt caatccagaa catcacggag tagaagtcac cggcaaacgc taacgacagc
     3121 ttgtcgcgat agataagctc gccaaagcgc agcacgatga aaatcgccag caacacactg
     3181 atggtgttgg tcagcttgac gaacagactc ttttcatccg gaccgttgcc acgcagaccc
     3241 gcctgcacca gcgaaccttc aaagatgaca atcgagaagc ccatgatgaa cgccgtcagc
     3301 agcgagaaca gcggcaacat ttcatagctc tgccacaacg gatgcacctt gtagcccgcc
     3361 gagatcatca gtgaccccat tgaagactgg tgcatggtcg gcaacagcgc gccaagtgca
     3421 atgatgaaga acataacctt gttcagacgc ttgagagaaa ccttccagcc cagacgttca
     3481 aacagtgccg gagcaaactc cagtgccatc acgccgatat agatggtcat acagaccgcc
     3541 gtctcgaaca gtaccgagtt cacgttgaag tgacccggaa tgtagaagta cggcaggttc
     3601 cagtagcgac ccacgtcgat agtgatcgac aagccaccca gtgagtaacc aaacagactc
     3661 gccaacagcg ccggacgcac cagcggatgg tattgcccac ggttaaagac gtataccgcc
     3721 cacgccagcg cccagccgcc acaggcaaag ccggtgccaa tcagcaggtc aaacgcgatc
     3781 cacacgcccc acgggaagcc gccgttcagg tcagagacag agcccagacc gaacaccaga
     3841 cgcttcacaa tcaggagcat acagatgacg attaacggtc caaaaatcat gaccggttta
     3901 ctgatgattt tgccgcccag cggttgtgga tcatgactca tgatcgtctc ctccgtcgtg
     3961 atggtcgttt ttggtgttgc gacgaaccag cacggttaag cccgccagca cagccagtgg
     4021 tagcatcatg cctttataca gggtgtgttg aatattttcg gaacgcgcac cggtagaaag
     4081 atcgtccagt ttcggcaggt cgagattttc ataaggcaca cccgtcagta ccagaacctg
     4141 agtaccgccg ccctctttct cgccgtacag atgcggataa tatttcggca ccgtatgcag
     4201 gtaagtgtcg ccagatttca gcgtctgacg cggatagtgg tattcgctgc caggcttcag
     4261 cgccagacgt tttttcgcct ccgccatcag ctcttcacgc gtaccgaaaa tcaccgcgcc
     4321 cgccgggcac acttctacgc agccaggcag accgccttta tcgagacgtt ccacaccttt
     4381 ctggttgcac agctcgcact tatgcagcgc accaaacggg ttgttgtagt cgtacttcgg
     4441 cacgttgtac ggacaggcga ccatgcagta acggcaaccg gtgcacacgt ctttgtcgta
     4501 atggacaatg ccggttttcg gatctttttt cagtgcagag accgggcaca cagagacaca
     4561 gttcggatcg acgcagtgca tacactgttt cttaatgtac gcgtagccgt tctcctcctg
     4621 gtctttgttg acccctgtgc cgctggtcca cacctgaatg atgttattgg tatacggcga
     4681 cagtttgtcg ttgttcgacc aggtctgttc cccttgcggg ttacgttcag ggaagttgat
     4741 atcctgacac ttggtgacgc aagcctggca gcctacgcac aaggtcgagt cgtacaacat
     4801 ccccagcgat cccggaattg gcgggcggtt ttcagcagcc gcatgactga cagacggcag
     4861 cgcgcccgtc agcaatgccc cgcaggaggc tgctttaata aaattacgtc tgttcacggt
     4921 tattctcccc gtgagtcagc gttatctttc ttttgctgac gacccagttc acgcaccgcc
     4981 atcacgctga caccggcaac cagcccaacc acaccgccga gcaaaccaat agcgcctgca
     5041 gagacgttgc cgccctcttt agcgttaaca tccggtttct gtgaacgcgg agtctgattt
     5101 tcgacgttgg caagctgatg gatgccttta tggaagccga taccttcttc gttacagcca
     5161 tagcatgggt gaccaatcgc taccggccac acgccgccaa catcgcagaa ttgcagcgtt
     5221 gagcagttgc cgtaagtttc tggcccttta cagccgaggt ggtacaggca ccagccttca
     5281 cggtggcctt catcaccgaa ctctttggca aaacgaccag catcgaagtg cgggcgacgt
     5341 tcgcagtgtt cgtgaatcag acggccatag gcgaaggtcg gacggttttt gtcatccagt
     5401 ttcggcggtt tgccgtaagt gatgatgtgc gcaacggtcg cgaggaagtt gtgcgggttc
     5461 ggcgggcagc ccggaatatt gataacggtt ttgcctggca gaacttcttg caggctgact
     5521 gcgccagttg ggttaactcc agctgcggca acaccgcccc acgcagagca ggaaccgata
     5581 gcgataatcg ctgctgcgcc ttctgccgct ttgcggatgt gatccacaat cggctcacca
     5641 gcaaccatgc aataaatacc gttatctttt aatgggatgg aaccatccac aaccaacaca
     5701 tactgccctt tgtacttctc aagtgcgtta tgtttgttct cttcgacctg atgaccgaag
     5761 gcggcggaaa gcacttcgtg atactccaga gagatagtct ccagcacgag gttttctacc
     5821 gttggatgcg ttgcacgaag cagagattcc gtacaaccgg tgcactcctg cgcgccaatc
     5881 cagataactg gcggacgctg cgggttagta accgattcgg ccatctctgc agcggctttg
     5941 ctacttaacc ccatggtggc ggctaatgct gcacaaagct tcatgaaatc acgacggtta
     6001 atgccgtgag aatggatgag ggtgttatct ccagtcattt atagttattc cgttgcgaag
     6061 acctggcata tattttgcct caatcgcaaa atcaataatg cgatcgatgc gccatttacc
     6121 acacatttat tatggttatc gatatcatga tactgcggcg gggcgctaaa acgaagggaa
     6181 gaagcattag tgtaattaat taaagcaaga taatacgtat gtttgatcaa ttttcgaggc
     6241 gataaccggc cataaaaaag cgcaacgtag aacaggaatt attctcacga aagaaatcca
     6301 ttaccatctc tttatccagt ccatagcggc gcgtcagaat acccgcttcc caggcgctaa
     6361 gctggcgatc tccggtcttt tcgtacatcc ggtgtgagta gcctaataca aaaccgcgtt
     6421 tataatcggc acagaatttt gtcacattaa ccgcactgtc ggcatgcgtt gcttttaatc
     6481 ccgccattaa gcctttacca aaatggttat tcatcgttca cctcattcgc tatatatcgc
     6541 catatatagc gatattttag gcaaagaacg acttttgcgc acgctatttt ttcatgcttg
     6601 tttaagacgt aatccagtaa gagataaacc tatcaggaga gatatttatc gaaccatttc
     6661 agtgtccttt gccaggcaag atcggcggca gatttgtcat aacggggcgt ggaatcatta
     6721 tggaatccgt gattaacccc cggatagata tacgcctcat aaaccttatt attggctttc
     6781 aacgccgcct cgtaagcagg ccagccctcg ttgattcggg tgtccagttc cgcgaagtgg
     6841 agtagtaaag gcgcttcaat cttcgccaca tcggcagtgg gtgcctgacg accataaaac
     6901 ggcaccgcgc aggccagttc cggatacgcg acagccgccg cgttcgatac gccaccgcca
     6961 tagcaaaatc cggtaatacc cactttgcct gccgcttgtg gatagcgttg cataaactca
     7021 attgcggcaa agaaatcatt catcagtttg gttggatcaa cctgctgttg cagctcacga
     7081 cctttatcat catttcccgg ataacctcca acggaactta agccgtcagg tgccagggcg
     7141 atataccccg ccttcgccac tcgccgtgcc acatcttcga tatacggatt cagtccacga
     7201 ttctcatgca ccaccaccac ggctggcgtt ttgccgctca tttttgcggg tttcaccaga
     7261 taaccccgta cctcgccgtg accatttggc gaaggataca tgatgtactc agcaacaatc
     7321 tccgggtcgg taaactctac ctgagtcgcc agcgcataat ttggcttgag caaatcaaac
     7381 aacgctaatg ccgtcatccc gcccaccgca tacttcgccg caaggttgag gaactcacgt
     7441 ttcgaaattt tcccgtgagc gtaatagtcg tagtaatcca acaactcttg tgggaaatct
     7501 ttggcggtta aacgcggcat cgttgcactc ctcagttggt gtttttttaa gcaaagcata
     7561 agcacgtgtt tttgcccagt ttttcgtcac tctgtgagcc agactacggg atacgcgctg
     7621 gcgaatcgct aaactagaaa cattgtttcg aaattgaacg gtggaaagga gaggtcatgg
     7681 tctggttagc gaatcccgaa cgttacgggc agatgcaata ccgctattgc ggaaaaagcg
     7741 gtttacgcct gcccgcgtta tcgctcggtt tatggcacaa tttcggtcac gttaacgcgc
     7801 tggaatcaca gcgtgcgatc ctgcgtaaag cgtttgattt gggcattacg cactttgatt
     7861 tagccaacaa ttacgggccg cctccaggaa gcgcagaaga gaactttggt cgcctgctgc
     7921 gggaggattt tgccgcttat cgcgatgaac tgattatctc taccaaggct ggctacgata
     7981 tgtggcccgg cccttacggc tctggcggtt cacgtaaata cctgctcgcc agcctcgacc
     8041 aaagcctgaa gcgtataggg cttgagtatg tcgatatctt ttactctcat cgcgtcgatg
     8101 aaaatacgcc gatggaagaa accgcctctg cgctggctca tgcggtacaa agcggtaagg
     8161 cgctgtatgt cgggatctcc tcttactcgc cagagcggac gcaaaaaatg gtcgagttgc
     8221 tgcacgagtg gaaaattccg cttttaattc atcaaccttc gtacaattta ctgaaccgct
     8281 gggtggataa aagcggcctg ctggataccc tgcaaaataa cggcgtgggc tgtattgcct
     8341 ttactcctct ggctcaggga ttgctgaccg gaaaatatct caacggcatt ccgcaagatt
     8401 cacggatgca tcgtgaaggg aataaagttc gtggtctgac gccgaaaatg cttaccgaag
     8461 ccaacctcaa cagcctgcgc ttattgaatg aaatggcaca gcagcgtgga caatcaatgg
     8521 cgcaaatggc gttaagctgg ttgctgaaag atgatcgcgt gacgtcggta ttgattggtg
     8581 ccagccgcgc ggagcaacta gaggagaacg tgcaggcgct gaataatctg acatttagca
     8641 ccgaggagct ggcgcagatt gatcagcata tcgccgatgg cgagctgaat ctgtggcagg
     8701 cgtcgtccga taaatgacct gttaataacg ggcgcgcgaa ccgcgcccga atcagatcag
     8761 tgattatgac gagtcagtcg gtcaagatag cccatcacaa atgcagaaag cacaaacgtc
     8821 agatggataa tgacgtacca catcagtttg ttatccggga catttttcgc atccataaag
     8881 acgcgcagta agtgaatgga agaaattgcc acaatcgacg ctgctacttt gtttttcagc
     8941 gacgttgcgt ccattttccc cagccagttc agcttctctt tgttctcgga gatatccagc
     9001 tgtgagacga aattctcata accggaaaac atcaccatca ccagcaaacc gccaaccagc
     9061 gtcatatcca ccagcgacag caacacgagg atcaaatctg attccgccat cgagaagata
     9121 ttcggcagta cgtgaataat ctcctggaag aacttcagcg ccagggcaac taacgccagc
     9181 gaaaggccaa agtacacggg ggcaagcagc cagcgagaag catacattgc attttcaaga
     9241 aaacgttcca tacattcctg tcgtgtagtt aaaacgggca acagtatatc gcaattgagt
     9301 aaacagcacg caacagcaaa tcaacatata cgttaaacct ttgtcgcata attctgaggt
     9361 ttagggatct tcctggttcc cttatcaacg catcgagagc gtctaccctt aaaggacgtt
     9421 gaacctgaag ggagaaaaac gatgtctcat ttaaaagacc cgaccacgca gtattacact
     9481 ggtgaatatc ccaaacagaa acaaccgacg ccaggcatcc aggcgaagat gacaccggta
     9541 ccggattgcg gcgagaaaac ctatgttggt agcggtcgcc tgaaagatcg taaagcactg
     9601 gtgacagggg gcgattccgg aataggtcgc gctgccgcca tcgcttacgc gcgtgaaggg
     9661 gctgacgtgg cgatcagtta tcttcccgtg gaagaagaag acgctcagga tgtgaaaaag
     9721 atcattgaag aatgcggacg caaagccgtt ctgctgccag gcgatttaag cgatgagaaa
     9781 ttcgcccgtt cgctggttca cgaagcgcac aaggcgttag gcgggctgga tattatggcg
     9841 ctggtcgccg ggaaacaggt tgccattccg gatattgcag acctcaccag cgaacagttt
     9901 caaaagacct ttgccattaa cgttttcgcg ctgttctggc taacccagga agcgatcccc
     9961 ctgctaccga aaggtgcaag tatcatcacc acttcgtcaa tccaggcata ccagccaagt
    10021 ccgcatttac tggactatgc ggctacgaag gcggcgattc tgaactacag ccgtggcctg
    10081 gcaaaacagg tcgcggagaa aggtattcgg gtgaatattg tcgcgccagg ccctatctgg
    10141 acggcactgc aaatttccgg cggacaaacg caggataaga tcccgcagtt tggtcagaaa
    10201 acgccgatga agcgtgcggg gcaaccggcg gaactggccc ctgtatatgt ttatctggca
    10261 agtcaggagt cgagctacgt caccgcagaa gtgcacggcg tgtgcggcgg cgagcattta
    10321 ggttaaaaaa atgcccggtt gtgaaaagca accgggcatc attgtgaatt cactcttacc
    10381 tgatgtggcg aaaacgcctt atccggccta caaacgcacg aagattcaac atgttgcaat
    10441 agattttgta ggcctgatga gcgtagcgca tcaggcaatt ctactttact tcgctttggc
    10501 ggtttcttcg ccgaccagac ctatcttcag gtaacccgcc tgatgcagcg tatccattac
    10561 cttcatcaac gtctcgtaat cgacggtttt gtccgctcgg aagaagatgg tggtgtcttt
    10621 cttgccttcg gttaacgcat tcaacgccgt aatcattgtt tcatcggtga ccggatcgtt
    10681 accgataaac atcgagttgt ctgccttcac cgacagataa accggttttt ccgggcgcgg
    10741 ctgcggcgtg ctggtagaag caggcaagtt caccttcaca tctaccgtcg ctaacggtgc
    10801 cgccaccata aagataatca gcagaaccaa catcacgtcg ataaacggcg tcacgttgat
    10861 atcatgcatt tcgccgttat cgtcgaggtt ttcgttaaga tgcattgcca tcggacatta
    10921 tcctgcgcgt aatttttgtg cgacacgaac cggatgcgca gcggcgctgg cttccagatc
    10981 caggtcacgg ctttgcagca acaatacctg cgctgcaaca tcacccagca tcgctttaaa
    11041 gccgccaatc tggcgtgcaa atacgttata gataacgacc gcaggaatcg ctgccacgag
    11101 gccgattgcc gttgctaaca gagcttctgc gatacccggc gcaacgactg ccaggttagt
    11161 ggtctgcgtt tgcgcgatac caataaagct gttcatgatg ccccagaccg taccaaacag
    11221 accaacgaac ggagaaatcg cgccgatggt tgccagatag ccgttaccgc ggcccatttg
    11281 acgacccact gcggcgaccc gacgctccag acggaagctg gtacgttctt taataccttc
    11341 gttatcgtcg ctgccttctg acagttccag ctcattctgc gcttcattga gcaaatgcag
    11401 gcttaagctt ttgctaccaa aatcagcggc gatatcgttg gcctggttta aggaacgcgc
    11461 ttcagccagc agttgctgct cgcgcttaag gcgacgcttc tgattgaaga actctacgct
    11521 cttactgaag aagattgccc aagtgactac ggaggccaaa ataagcccaa tcatcacgca
    11581 cttaacgaca atatcggcgt gctgatacat accccagacg gaaaggtccg tctgcatcaa
    11641 attattaccc actgtgtatc tccaggacgc aagtcacaaa atctgcgcat aataatatca
    11701 aaatgacgtc gaattgatag tcgttctcat tactatttgc atactgccgt acctttgctt
    11761 tcttttcctt gcgtttacgc agtaaaaaag tcaccagcac gccatttgcg aaaattttct
    11821 gctttatgcc aattcttcag gatgcgcccg cgaatattca tgctagttta gacatccaga
    11881 cgtataaaaa caggaatccc gagatgacgg acaaaaagct tgatactcaa ctggtgaatg
    11941 caggacgcag caaaaaatac actctcggcg cggtaaatag cgtgattcag cgcgcttctt
    12001 cgctggtctt tgactgtgtg gaagccaaaa agcacgcgac gcgcaatcgc gccaatggcg
    12061 agttgttcta tggacggcgc ggaacgttaa cccatttctc cttacaacaa gcgatgtgtg
    12121 aactggaagg tggcgcaggc tgcgtgctat ttccctgcgg ggcggcagcg gttgctaatt
    12181 ccattcttgc ttttgtcgaa cagggcgatc atgtgttgat gaccaacacc gcctatgaac
    12241 cgagtcagga tttctgtagc aaaatcctca gcaaactggg cgtaacgaca tcgtggtttg
    12301 atccgctgat tggtgccgat atcgttaagc atctgcagcc aaacactaaa atcgtgtttc
    12361 tggaatcgcc aggctccatc accatggaag tccacgacgt tccggcgatt gttgccgccg
    12421 tacgcagtgt ggtgccggat gccatcatta tgatcgacaa cacctgggca gccggtgtgc
    12481 tgtttaaggc gctggacttt ggcatcgatg tttctattca agccgccacc aaatatctgg
    12541 ttgggcattc agatgcgatg attggcactg ccgtatgcaa tgcccgttgc tgggagcagc
    12601 tacgggaaaa cgcctatctg atgggccaga tggtcgatgc cgataccgcc tatataacca
    12661 gccgtggcct gcgcacatta ggtgtgcgtt tgcgtcaaca tcatgaaagc agtctgaaag
    12721 tggctgaatg gctggctgaa catccgcaag tagcgcgagt taaccaccct gctctgcctg
    12781 gcagtaaagg ccacgaattc tggaaacgag actttacagg cagcagcggg ctattttcct
    12841 ttgtgcttaa gaaaaaactc agtaatgaag agctggcgaa ctatctggat aacttcagtt
    12901 tattcagcat ggcctactcg tggggcgggt atgaatcgtt gatcctggcg aatcaaccag
    12961 aacatatcgc cgccattcgc ccacaaggcg agatcgattt tagcgggacc ttgattcgcc
    13021 tgcatattgg tctggaagat gtcgacgatc tgattgccga tctggacgcc ggttttgcgc
    13081 gaattgtata acattgccac ttttggacaa ttttgcagac atttaattgt gaaaagtctt
    13141 aaattgttgc gtccgggatc aaggcgtccc ggacgaatca ggagtacaat aggcagataa
    13201 aggcttaaac gctgttccac aggaaagtcc atggctgtta ttcaagatat catcgctgcg
    13261 ctctggcaac acgactttgc cgcgctggcg gatcctcata ttgttagcgt tgtttacttt
    13321 gtcatgtttg ccacgctgtt tttagaaaac ggcctgctgc ccgcctcatt tttgccaggc
    13381 gacagcttgt tgatactggc aggcgcattg attgcccaag gggttatgga ttttctgcct
    13441 acgattgcga ttctgaccgc cgcagcaagt ctgggctgct ggctaagtta tattcagggg
    13501 cgctggttag ggaataccaa aacggtgaaa ggctggctgg cacagcttcc tgctaaatat
    13561 caccagcgcg ccacctgcat gtttgaccgc cacggtctgc tggcgctgct ggctggacgt
    13621 tttcttgcat ttgtccgtac gctgctgcca accatggcgg gaatttccgg tctgccaaac
    13681 cgccgcttcc agtttttcaa ctggttaagt ggattgctgt gggtcagcgt ggtaaccagt
    13741 tttggctatg ccttaagtat gattccgttc gttaaacgcc atgaagatca ggtaatgacg
    13801 ttcctgatga tcctgccaat tgccttgtta accgctggct tgttaggcac gctgtttgta
    13861 gtgattaaaa aaaaatactg taacgcctga cgattttccc cgttcccggc tgctgtaccg
    13921 ggaacgtatt taattcccct gcatcgcccg cattcttgcc gcatcttccc ccggcgtcac
    13981 accgaagtaa cgtttaaact cacggctaaa ttgcgatgcg ctttcatagt cgacgcgcat
    14041 cgctgctgcg ctggccttca tgccgtcatg gatgatcatc atccgcgcct tatgcagacg
    14101 gtaattcttc aaatactgca acggcgaggt gctggtgaca gacttaaaat tatgatggaa
    14161 cgccgatacg ctcatgttgg cttctgccgc cagttgctcg acgctcaggt tttcggtgta
    14221 tttattctca atccgtttca gcacgcggct aatcagactg aagtgagtct ggcgactgac
    14281 cagcgccagt aacgcgccgc cgcaaggtcc ggtcagcacg tagtacagaa tttcgcggat
    14341 gatctgtttg ccgagaatac gcgcatccag tggtcgctcc atcacgtcga gtaaccgctc
    14401 cgccgcgcat aaaatctctt ctgataacgt ggcggagtta atcccgctgg ctgccatcga
    14461 cggctggaaa tgctcatctt cgccaatgtc catcaacagt tcctgtaact gcaaaatatc
    14521 gacattgaga cgcaatcctg ccagcggcac ctctgacgtg gcataggttt cgcactcaaa
    14581 cggcaacggc accgtcagca gcaggtattc attggcatca taacgaaaca cgcgttcatt
    14641 gatataaccg attttatgcc cggaaaagag aattatgatg ccaggctcgt acatcaccgg
    14701 tgtacgtgcg aaaggcgtct cgccatacaa caaacgcaca tcgggcaaca gttctgacaa
    14761 actattttct ttatttttca gtttattaac tttatccgcc agcaagcggc aaatctcttc
    14821 acgtttcata tcgcgtaatt tcttaggaat aatgcggcaa tttgattgtg cgcaattttg
    14881 tagcatttct ccagcactct ggagaaatag gcaagacatt ggcagaaatg agcattgaga
    14941 gccagggcgc tggcgatcac aatgaaaaac atcaggcaga tcgttctctg ccctcatatt
    15001 ggcccagcaa agggagcaag taatgaacaa ctttaatctg cacaccccaa cccgcattct
    15061 gtttggtaaa ggcgcaatcg ctggtttacg cgaacaaatt cctcacgatg ctcgcgtatt
    15121 gattacctac ggcggcggca gcgtgaaaaa aatcggcgtt ctcgatcaag ttctggatgc
    15181 cctgaaaggc atggacgtgc tggaatttgg cggtattgag ccaaacccgg cttatgaaac
    15241 gctgatgaac gccgtgaaac tggttcgcga acagaaagtg actttcctgc tggcggttgg
    15301 cggcggttct gtactggacg gcaccaaatt tatcgccgca gcggctaact atccggaaaa
    15361 tatcgatccg tggcacattc tgcaaacggg cggtaaagag attaaaagcg ccatcccgat
    15421 gggctgtgtg ctgacgctgc cagcaaccgg ttcagaatcc aacgcaggcg cggtgatctc
    15481 ccgtaaaacc acaggcgaca agcaggcgtt ccattctgcc catgttcagc cggtatttgc
    15541 cgtgctcgat ccggtttata cctacaccct gccgccgcgt caggtggcta acgacgtagt
    15601 ggacgccttt gtacacaccg tggaacagta tgttaccaaa ccggttgatg ccaaaattca
    15661 ggaccgtttc gcagaaggca ttttgctgac gctaatcgaa gatggtccga aagccctgaa
    15721 agagccagaa aactacgatg tgcgcgccaa cgtcatgtgg gcggcgactc aggcgctgaa
    15781 cggtttgatt ggcgctggcg taccgcagga ctgggcaacg catatgctgg gccacgaact
    15841 gactgcgatg cacggtctgg atcacgcgca aacactggct atcgtcctgc ctgcactgtg
    15901 gaatgaaaaa cgcgagacca agcgcgctaa gctgctgcaa tatgctgaac gcgtctggaa
    15961 catcactgaa ggttccgacg atgagcgtat tgacgccgcg attgccgcaa cccgcaattt
    16021 ctttgagcaa ttaggcgtgc cgacccacct ctccgactac ggtctggacg gcagctccat
    16081 cccggctttg ctgaaaaaac tggaagagca cggcatgacc caactgggcg aaaatcatga
    16141 cattacgttg gatgtcagcc gccgtatata cgaagccgcc cgctaagctt tttacgcctc
    16201 aaactttcgt tttcgggcat ttcgtccaga cttaagttca caacacctca ccggagcctg
    16261 ctccggtgag ttcatataaa ggaggaacgt atggctaatc caaccgttat taagctacag
    16321 gatggcaatg tcatgcccca gctgggactg ggcgtctggc aagcaagtaa tgaggaagta
    16381 atcaccgcca ttcaaaaagc gttagaagtg ggttatcgct cgattgatac cgccgcggcc
    16441 tacaagaacg aagaaggtgt cggcaaagcc ctgaaaaatg cctcagtcaa cagagaagaa
    16501 ctgttcatca ccactaagct gtggaacgac gaccacaagc gcccccgcga agccctgctc
    16561 gacagcctga aaaaactcca gcttgattat atcgacctct acttaatgca ctggcccgtt
    16621 cccgctatcg accattatgt cgaagcatgg aaaggcatga tcgaattgca aaaagaggga
    16681 ttaatcaaaa gcatcggcgt gtgcaacttc cagatccatc acctgcaacg cctgattgat
    16741 gaaactggcg tgacgcctgt gataaaccag atcgaacttc atccgctgat gcaacaacgc
    16801 cagctacacg cctggaacgc gacacacaaa atccagaccg aatcctggag cccattagcg
    16861 caaggaggga aaggcgtttt cgatcagaaa gtcattcgcg atctggcaga taaatacggc
    16921 aaagccccgg cgcagattgt tatccgctgg catctggata gcggcctggt ggtgatcccg
    16981 aaatcggtca caccttcacg tattgccgaa aactttgatg tctgggattt ccgtctcgac
    17041 aaagacgaac tcggcgaaat tgcaaaactc gatcagggca agcgtctcgg tcccgatcct
    17101 gaccagttcg gcggctaaca tgcaaattct cccggtgacg gtaatgttcc gctaccggac
    17161 ttttcagaaa tcatttattc ccctcgcgtc ccgcccgttg ttactcttcc ttgttcagga
    17221 atgccaaata taaggacatc atcatgcaga gccggaagct cttaaaagaa caactcatct
    17281 atatccggga taaacgcaac ggagaggtga aaaacagatg aaaataatac ttctgttttt
    17341 agcagccctg gcaagtttta ccgtacacgc gcagcctcct tcacagaccg tagaacaaac
    17401 agtccggcag atttatcaga actataaatc agatgccagt acaccttatt tcggtgaaac
    17461 cggagagcgg gcgataactt cagcgcgtat tcaacaggcg cttaccctga acgacaatct
    17521 tacgctgccg ggcaatattg gctggctgga ttatgatccg gtttgtgatt gtcaggattt
    17581 tggcgatctg gtgctagaaa gcgttgcgat aacccaaact gacgccgatc atgccgatgc
    17641 cgttgtgcgc tttcgtatct ttaaagatga taaagaaaag accacgcaga cactgaaaat
    17701 ggtggcggaa aatggtcgtt gggtcattga cgatattgtc agcaatcatg gcagcgtctt
    17761 acaagcagtt aatagcgaga atgaaaaaac gctggccgct ttagcttcgt tgcaaaaaga
    17821 acagccggaa gcctttgttg ccgaactctt tgaacatatt gctgattata gctggccgtg
    17881 gacgtgggtg gtttccgact cttaccgcca ggcggttaat gccttctata aaaccacctt
    17941 caagacggcc aataatcccg atgaagatat gcaaatagaa cggcaattta tttacgacaa
    18001 tccgatctgt tttggcgaag agtcgctatt ttcacgcgtt gatgaaattc gagtcctgga
    18061 gaaaaccgcc gattccgccc gcattcatgt tcgttttacg ctgaccaatg gcaacaacga
    18121 agagcaagaa ctggttttac agcggcgcga aggcaagtgg gaaatcgctg attttatccg
    18181 cccgaacagc ggcagcctac ttaagcagat tgaggcaaaa accgccgcca gattaaagca
    18241 atgagctgaa ttaaataaca attagccgga acaataaata aaagggaaca ctatatgaaa
    18301 acgattttca ccgtgggagc tgttgttctg gcaacctgct tgctcagtgg ctgcgtcaat
    18361 gagcaaaagg tcaatcaact ggcgagcaat gtacaaacat taaatgccaa aatcgcccgg
    18421 cttgagcagg atataaaagc actacgccca caaatctatg ctgccaaatc cgaagctaac
    18481 agagccaata cgcgtcttga tgctcaggac tattttgatt gcctgcgctg cttgcgtatg
    18541 tacgcagaat gataaaaaaa tccccggcag catgtcagtt gccggggatt ttttttaacg
    18601 tccaaccgcc gctttagggc gtttcttcgc accagcattc accggacgag attgcgtaga
    18661 cgacgctttt tttgccgtag caggcgtctg acgctgggtc gccatcggcg tatgtttagt
    18721 caacgccgga cgggtattgc ggttctggcg acgagcttca cgcatctctt caatggttgg
    18781 cgcaggcact aagcaatcgc gacggctgcc aatcagatgc tttttgccca tcgcttccag
    18841 cgccgggcgg attaacggcc agtttgccgg atcgtggtaa cgcaacaacg ctttatgcaa
    18901 acgacgctgt ttgtcgccct tcggtacgaa gacgtcttcg ctcttataac caatcttcgc
    18961 cagcggtttt ttcccggtgt aatacatggt ggttgagttc gccagcggcg atgggtagaa
    19021 gttctgtacc tggtcgagac ggaaacgatg ctttttcagc cacagcgcca aattcaccat
    19081 atcttcatca cgcgtaccgg ggtgcgcaga gatgaaatac gggatcagat actgctcttt
    19141 acctgcctgt ttcgagtagg tatcgaacag ctctttaaag cggtcatagc tgcccatgcc
    19201 cggcttcatc atcttcgaca acggcccttc ttcggtatgt tccggagcaa tcttcagata
    19261 accgccgacg tgatgggtcg ccaactcttt gatatagcgc ggatcttcca cggcgatgtc
    19321 ataacgtacg cctgaggcga tgaggatttt tttaatgcct ttcagatcac gcgcacggcg
    19381 atagaggttg atcgtcggtt cgtggttagt gtccatgtgc ggacaaatat ccggataaac
    19441 gcacgacaaa cggcgacaag tttgttcagc gcgtggcgat ttgcagtgca acatatacat
    19501 gttggcagtt ggcccaccga gatcggaaat cacgcccgta aaacctggaa cggtgtcgcg
    19561 gatcgcttcg atctcattaa tgatcgaatc ttccgaacgg ctctgaataa tgcgcccttc
    19621 gtgctcggta atagaacaga aagaacagcc gccaaagcag ccgcgcataa tgttgaccga
    19681 aaaacggatc atttcgtaag ccggaatacg ggcattgcca taggccggat gtggcacgcg
    19741 cttgtacggc agcgcaaaaa cgctgtccat ctcttcggta gaaagcggga tagcaggcgg
    19801 gttgatccac acatagcggt caccgtgttt ttgcatcaat gcgcggacgc agcctgggtt
    19861 ggtttcgtgg tgcagaatac gcgaagcatg ggcgtacagc actttatcgc ccttcacttt
    19921 ctcgaaagaa ggcagcaaca cgtaggtttt ttcccacggt ttcgggcgcg gtggctgcac
    19981 ggttacggct ttggcttcct gctttttcgg tgccaccggt ttgttatccg cgcacggcaa
    20041 atcttcacca tacggatgcg ggattgggtc gatttttcca ggggtatcaa gacgggggga
    20101 atccacgccg ctccagccag gcagcgcctc tttcacgata atcgcggtat tacgcacatc
    20161 gcggatttca ctaatcggct cgcccatcgc cagacggtgc gccacctcca ccagcggacg
    20221 ctcaccgtta ccaaacatca gcatgtcggc tttcgaatcc accagcacgg aacggcgcac
    20281 ggtatcggac cagtaatcat aatgtgcggt acggcgcaga ctagcctcaa taccgccgag
    20341 gatcaccggt acatctttcc acgcctcttt acaacgctgg gtataaacca gtgtggcgcg
    20401 atccgggcgc ttacccgcga cgttatccgg cgtgtaggca tcgtcatgac gtaaacggcg
    20461 atcggcggta taacggttga tcatcgaatc catgttgcca gcagtaacac cgaaaaacag
    20521 attcggttta cccagacgca taaagtcgtc tttgctgctc cagtccggct gggcgatgat
    20581 cccgacgcga aagccctgtg cttccagcat acgaccgcaa atcgccatcc cgaagcttgg
    20641 gtgatcgaca tacgcgtcgc cagtaaccaa aatgatgtcg cagctatccc agccaagttg
    20701 atccatctct tcacgagaca tcggcaaaaa cggtgccggt ccaaaacagg cggcccagta
    20761 ctgcggccag gagaacaggt cgcgatccgg ttggatcagg gagatagagc tcattttgct
    20821 tccagaaatg ataaaaaaat aatcaaaggc cggggattat aagccggaac gaaagagaaa
    20881 tcgaaaagta ttccatactc gccctcctcg ggcgagtatg aagattacgg taccggattg
    20941 actaacagtt gcccaatcga gccacggtcc gccatttcca gcgtctgact gttgaagtag
    21001 aacgggaagt gcgcccagga aggctgaccg aaataaacaa gcagctccac ttgtccgtca
    21061 acccaaacgg tatctttcca gcctctgtct tccgggaacg gcatcgcacc attcacgtta
    21121 cggatctgga acatcacgcc ttcaatatgg aacgcttgcg gctcgtccgc gcgtaccgtc
    21181 cagcgttccc acgttccttg ctgcgcggtg acatcaatac ggttgacgtc ccacagctgt
    21241 ccattaatac ccgggtcatc acccagactg atatcgcgac tgcgaatcgg cgaaccggcc
    21301 atgatttcag ttggcagcaa gcgcatcgga agactgtctg tgaccagcgg cagaaggccg
    21361 gttgggcgca gcgttagcac cagggtagaa accagaatac tggatggctc aaagaagccg
    21421 cgaatacgat caacaatgct cgccgcttcg ccacaggtga tcgacacttc atcgccgttg
    21481 ctcatatcca ccagaatctc gcggcgctcg cccggtgcca gcgaaagttg cttcaccgat
    21541 acaggagcag gcaggaatcc ctgatcgcca gaaatcacat gtaacgggcg accatcgctc
    21601 atctgtagtt gatagcgacg agagttcgac gcgttcagca gtcgcaagcg cacccagcca
    21661 cgcgagactt caacgtacgg gctttgtaca ccgttaacca gcagcgtatc accaacaaag
    21721 ccgccgcttc ccggttcgtt gtattctggc gtaccaaagt tatccagccg tttatcctgg
    21781 ataatgaccg gaaaatcatc cacatcataa tggttgggga taggcagcga cttgctgact
    21841 tcatcttcca ccagccacat tccggcaagg ccgttataga cctgctgagc cgtgcggttg
    21901 ggagtattgg cgtgatacca cagagtagct gcgttctggc gaataggcag tacgggtgcc
    21961 cagtcagcgt ttggcgacat catccgtgcc ggaccgccca tcagcgggcc aggtacctgt
    22021 agcccggcca ccgtcattga gacattttct gtcaggcggt tgctgtaaat aagcttaaca
    22081 tcgtcgccct tccagacgcg gatagtcggc cccaggtaac gaccattgat tccccagacc
    22141 gacgcgcgtg tccctggcgt aaatgaccag tgcgcacgtt gtacagtcat aaacagcggt
    22201 tgcccacggc gagattcaag tagcggcgga acgggtagcg gttgctgttg cccggctgcg
    22261 ctggccttca ggggaacagc gcctgcacaa agtgcaatcc ccgatgcctg aatgaactga
    22321 cgccgactga gtgacatatt tgctccatgt aaaactggct aaagtaatac cgtgcaggaa
    22381 agtgttcccc gcaaaaatac agattgccga cttaaacttt tccggcggct tcgcgttctg
    22441 cgacttcttt atcgagctcg gcgatttttt gttccattat cgaacgacaa tgggcagcca
    22501 gctcacgaac ctgatctttg ccatactgac tgacgtcaat tggcggcagc atttcgacaa
    22561 tcaccagacc gttgtgcagt cgattaagat taatcttatt cgaagttgta gagacgcaca
    22621 cgggaataat cgggacgccc gccgcaattg ccgcgtgaaa tgctccagtc ttgaacggta
    22681 gcaggccgcg accacggctg cgggttcctt ccgggaacat ccagatggaa atacggcgtt
    22741 ttttgaaatg attcacgact tccgcaatgg tgccgtgagc tttagtgcga ttgtttctgt
    22801 cgatcaataa gttgccggtt aaccagtaca actgcccgaa gaaggggatc cacagcaagc
    22861 tctttttacc taccgtcacc gtcggcggtt gcacaatgtt cgatgctgtc accatgtcat
    22921 agttgttctg gtggttagcg atatagatag cattgccgta gctttcagca tctgcaggtt
    22981 tgcggcactc aactttcagg ccgaacagcg gcgcaagacg gccaaacata tgcccaaagg
    23041 tggccacatg tttcgggtta cgcgggctga aaaggcagta aatggagccg aatacacaga
    23101 ctaagatgct gtaaatcacg gtaataataa gacgaaagat atatagcata acaccctctg
    23161 aaggtctgag agactggcat tgtacgtcac ctgaaatcag gttagggcat tgttaaaagc
    23221 cgctctctgg aaaagcgtat tgttaatcgc aacgcatgaa taaacaacgg tttcaaggga
    23281 atttttatcg tttccggggg ttgaatacgc tgccggatga cgacttaacg tttcatccgg
    23341 cgttccttgc aagcgggagg aaacaggccc tccccggcat attactcttc gctatcaccg
    23401 ttgctggcac ggcgaggaga gtcgatctca acacgatcga tacgctgcaa accgcgcatc
    23461 aacgtaccgc ggcgtccacg ttcaccagtg actttctgta actcttccgg gcgcagttta
    23521 attttgcgtt tcccaacatg aatggtcagc gtgctttgcg gcggcagaac gtacaattgc
    23581 gccagaccat cttctccacg cgcggcttct gccgatggaa tgttgataat cttgttgcct
    23641 ttgcccttcg acagctgcgg cagatcgctt accgggaaca tcaacatacg gcctgcctga
    23701 gtgattgcca gcagcatatc ggaagcatct tcaatcacca ccggcggcat aacatgggca
    23761 ttttccggta aggtgatcaa agccttacct gcacggttac gcgccaccag atcgttaaag
    23821 gtgcagacga aaccgtaacc cgcatcggaa gccatcagca gtttctgatc gtcgctttcc
    23881 atcagcatat ggtcaacggt cgccccaggc ggcaacgtta atttgccggt gagtggttcg
    23941 ccctgaccac gcgccgacgg cagcgtaatc gggtcgatgg catagctacg accggtggaa
    24001 tcaacaaaca ctaccgattg gttgctctta cctttcaccg ccactttgaa gctatcaccc
    24061 gctttataat tcaggcccgg cgcgtcgata tcatggcctt tagcgctgcg tacccagccc
    24121 atctgcgaca gcacaatggt gacaggttca gacggcagca tgtcgtgctc gctcatcgct
    24181 ttcgcttctt cgcgttcctg caacggcgaa cgacgatcgt caccgtaggc ttgcgcgtct
    24241 gcctgcagtt ctttcttcag caggttattc attttacgct cggaagccaa aatgccctgc
    24301 aactggtcgc gctctttttc cagctcactc tgctcaccgc gaatcttcat ctcttccagt
    24361 ttggcaagat gacgcagttt cagttcgagg atcgcttccg cctgggtttc cgtaaggcca
    24421 aaccgcgaca tcagcgccgg tttcggttca tcttcgttac gaatgatctc aatcacttcg
    24481 tcgatgttga gaaacgccac cagcaaacct tccaggatat gcaggcgctt gaggactttc
    24541 tccagacgat agttcagtcg gcggcgcacg gtatcgcgac ggaacaccag ccattcggag
    24601 aggatttcca gcaggttttt caccgccgga cgaccatcca gaccgatcat attgaggtta
    24661 atgcgatagc tcttttccag atcggtggta gcgaagaggt ggttcatcac ctgatccata
    24721 tccacgcggt tggaacgcgg cacaatcacc agacgggtcg ggttctcgtg gtcagattca
    24781 tcgcgcagat cgtcaaccat cggcagcttt ttgttgcgca tttgcgcagc aatttgctcc
    24841 agtacacgcg cacctgaaac ctgatgcggt aatgcgctga taaccaccgc gccatcttct
    24901 ttcttccaca ccgcgcgcat acgtactgaa ccacgtccgt tctcgtagat tttacggatc
    24961 tcggcgcgcg aagtgataat ttccgcttca gtcggataat ccggcccctg cacgatatcc
    25021 agcagctgat cgagcgtggt tttcggctgg tcgattaatg cgattgccgc ctgagctact
    25081 tcacgcaggt tatgcggtgg aatatcggtc gccatgccga cggcaatacc ggtggtgccg
    25141 ttaagcaaaa tgtttggcag acgagcaggc agcattttcg gctcctgcaa agtgccgtcg
    25201 aagtttggca cccagtcagc cgtcccctgc cccagctcgc tcaatagcag ctcggaatat
    25261 ttcgacaacc gggattcggt gtaacgcatt gccgcgaacg atttcggatc gtccggcgcg
    25321 ccccagttcc cctgaccatc aaccagcgga taacggtaag agaacggctg cgccatcagg
    25381 accatcgctt cataacaggc gatatcgccg tgcggatggt atttacccag tacgtcaccg
    25441 acggtacggg ccgatttttt aaatttggcg ctggcattca ggcccagttc agacatcgca
    25501 tacacaatgc ggcgctgaac aggtttcaga ccatcaccaa taaacggcaa cgcacggtcc
    25561 atgatcacgt acatggagta gtttaagtag gcgttttccg taaattcatg tagcgcaagg
    25621 cgctctgcca tatcgctcat taattctgat tcctcaactg attcgccagc cttcaaaggg
    25681 gcaatattgc cggagatact acattatctg ccgcgtcgag tcacaggaga tcagcgtgaa
    25741 tagcgccgcc ggacgtgctc cgacggcgat aaaggcatca atgtgccttg atatacaacg
    25801 ctttgctgta gacttgatcg gcatagaggg cagcacaagg ttgcgcccat gccacaccac
    25861 tcggcatggg ggtccgctcc gggcgattcg agaagttgag gttatgcgag tccagggaag
    25921 cctaaagcat caaagtagtc gactgtaagt atatggttca gcccagttta gctgtgtctc
    25981 caccagtgcc ccgcgcctcc tgccataagt gcggcttgct catgggtacc cccgagacgg
    26041 cttacggcgc accggataat aaatttcgcg tatacatggt attccttacc ggagaaatta
    26101 taagcccaac aacaatacgc ctgtggcatt gttcgctcaa acttatcgcg agtgatttgg
    26161 ctcacactcc ggtaaagatt ccccatcacg ctaacggcac ataaacatcc gtttccagtc
    26221 gctgctccgt caccccttcg gcaagattgg tgtagtgaaa cagaatcggt gctttacgca
    26281 ttttctcgcc gcttgcaggc agccagtggc gaataatgcc ccatatcgtg tgtgaaatat
    26341 cgtccagctc gccaacgtgg cgggccacgg cataacgtcc accggtaagt tcaccattgc
    26401 tcacaccata acgattatcg ggaatcggtt cgctaacgct gccgcagata tcaaagcgaa
    26461 acgcttccgg tgcggtggtg gcagggtcgt cccaggcgac gccaaaagtc tggctttggt
    26521 taacaggaga aagccccgtc tctttacgcc acatgataaa tttcgccaca ctgtagttga
    26581 gcaactcagg gctacagcgg tgcggcaaca tcgccacagg gattgatggg aaatcgataa
    26641 tgtttacatc cagtgtcagg tttgtcattt tgcctccctg cgaaattatt cccgggaacg
    26701 cgtcccggga gcggtaacaa ttacggattt actttgcgga tctgtttgac gtcaatttca
    26761 acagaattcc agtctttatc gacttcaccc tgaatctcaa ccgtatcttt cggcgtcacc
    26821 gtcacaccgt tccagcgttt gtggtcgata tcaacattga tagtaccgct ggcatcttta
    26881 aacacgtaga gatcgtcaga gatgcgttca acgatattgc cgcgcagggt tacccaggtg
    26941 tcgtcacgca gggattttgc gctttctaca gtcgttacgc tgccgttcgg cccctggaat
    27001 cctccggcct gactttgcgt tgccgatggg ccagaaaaac cgccctgctc tgctgccatc
    27061 accggtgcac tgcacagggc cattactgcg attactgctg cgaatttttt catgtttatt
    27121 actcccttta atgtctgttt ccgagcattt aacaagatag tccttaacaa cttcttaagg
    27181 gaaaaaaata aaatttaatg ctgtacagag cgcgttacaa cacggtttac tggcagcaaa
    27241 tacggttatc gcagggatga aaaaatgcga attttactga tagaagatga catgctgatt
    27301 ggcgacggca tcaaaactgg ccttagtaaa atgggtttta gtgtcgactg gtttacacaa
    27361 ggtcgtcagg gaaaagaggc gctttatagc gcaccttatg atgcggtgat cctggattta
    27421 accttaccag gcatggatgg tcgcgatatt ttgcgcgaat ggcgagagaa aggtcagcgt
    27481 gagccggtac tgatcctgac cgcgcgtgat gcgttagcgg aacgtgtaga agggctgcgt
    27541 ctgggagctg acgattatct gtgtaaacct tttgcgttga tagaagtcgc cgccaggctg
    27601 gaggctctga tgcgccgaac caacggccag gccagcaacg agctgcgcca cggtaacgtc
    27661 atgctcgacc ccggcaaacg tatcgccacg ctggctggcg aacccttaac actgaaacca
    27721 aaagaatttg ccctgctgga attactgatg cgtaacgctg gtcgggtact gccgcgcaaa
    27781 ctgattgaag agaaactgta tacctgggac gaagaggtca ccagtaatgc cgttgaagtg
    27841 catgtgcatc atctgcgacg caaactcggt agtgatttta ttcgtaccgt gcatggtatt
    27901 ggctacacat taggtgagaa atgaaattta cccaacgtct tagtctgcgc gtcaggctga
    27961 cgctaatctt tttaattctg gcctcggtga cctggctgct ttccagcttt gtcgcctgga
    28021 aacaaacaac ggataacgtc gatgaattgt tcgacaccca actgatgctg tttgccaagc
    28081 ggttaagtac gctcgatctc aacgaaatca acgcggcgga tcgcatggca cagacgccaa
    28141 ataaattaaa acacggtcat gttgatgacg atgcgctgac ctttgccatc tttacccacg
    28201 acggcagaat ggtccttaat gatggcgata acggagaaga tattccctat agctatcaac
    28261 gggaaggttt tgctgacggg caactggtcg gtgaagacga tccttggcgt tttgtctgga
    28321 tgacctcacc tgatggcaaa tatcgcatcg ttgttggcca ggaatgggaa taccgtgaag
    28381 acatggcgct ggcgattgtt gccgggcaat tgatcccgtg gctggtcgca ctgccgatta
    28441 tgttaatcat catgatggta ctactgggtc gtgaactcgc gccgctgaac aaactggcgc
    28501 tggcactacg tatgcgtgac cctgactcgg aaaaaccact aaacgcgact ggcgtaccca
    28561 gcgaagtgcg accactggtt gagtcgctaa atcaactgtt cgcccgcaca catgcgatga
    28621 tggttcgaga acgacgcttt acctccgatg cagctcacga actacgtagc ccgttaacag
    28681 cgctgaaagt gcaaaccgaa gttgcgcagc tctctgacga tgatccgcag gcgcggaaaa
    28741 aagcactgct ccaattacat tccgggatcg atcgcgctac tcgtctggtt gatcaactgc
    28801 tcacgctatc gcggctggac tcactggata accttcagga cgtcgcggag atcccgcttg
    28861 aagatctcct gcaatcgtcg gtgatggata tttaccacac ggcgcagcag gcgaaaattg
    28921 acgtgcgact gacactcaat gcccacagca tcaaacgcac tggacaaccg ctattgctaa
    28981 gtttgttggt gcgaaacctt ctggataacg cagtgcgcta cagtccacag ggcagcgtgg
    29041 tggacgtcac gctgaatgcc gataatttca tcgtgaggga taacggcccc ggtgtgacac
    29101 cagaggcact ggcgcgaatt ggcgaacgct tctatcgccc ccccggacaa accgctaccg
    29161 gcagtggact tgggctatcg attgtccagc gaatcgccaa attgcatggc atgaatgttg
    29221 aatttgggaa tgcggaacaa ggtggatttg aggcgaaggt gagctggtaa gggtaagact
    29281 tttgctaaat tcgcaaaaga ctttgcacat tttgctaatt ttaccgtacc gctctgtgac
    29341 gtactatagt cgacaaacgt ctcaccttga ggttaaaaaa tgagcaacac cctgattatc
    29401 aacggcgcga aaaaattcgc ccactccaat ggtcaactga acgacaccct gaccgaagtc
    29461 gcggatggca cactgcgcga cctcgggcat gatgttcgca tcgttcgcgc cgacagcgac
    29521 tacgatgtca aagcggaagt acaaaacttt ctctgggctg atgtggtgat ctggcagatg
    29581 ccaggctggt ggatgggcgc gccgtggaca gtgaaaaaat acattgatga tgtattcacc
    29641 gaaggtcacg ggacgctgta tgccagcgat ggccgtaccc gcaaagatcc gtcaaaaaaa
    29701 tacggttccg gcggcctggt acagggcaaa aaatatatgc tttctctgac ctggaacgca
    29761 ccaatggaag ccttcaccga aaaagatcaa ttcttccacg gcgttggcgt cgacggtgtg
    29821 tatctgccgt tccataaagc aaaccaattc cttggcatgg aaccgctgcc gacatttatc
    29881 gctaatgacg tgataaaaat gcctgatgtt ccccgctata ctgaagaata tcgcaagcat
    29941 cttgtggaaa tttttggtta actagagctc aggctttaga aggagttaac catgcttacc
    30001 gtaatcgcag aaatccgtac tcgtcctggt caacatcacc gtcaggcggt attggatcag
    30061 tttgctaaaa tcgttccaac cgtactgaaa gaagaaggtt gccacggcta tgcgccaatg
    30121 gtggattgcg ctgctggcgt gagtttccag tctatggcac cggattctat cgtgatgatt
    30181 gagcagtggg aaagcatcgc gcaccttgaa gcgcatctgc aaaccccgca catgaaggcg
    30241 tacagcgaag ccgtaaaagg cgacgtgctg gagatgaata ttcgtattct gcagccaggg
    30301 atttaatcct gccttgtttg cccggccatc ctgaccgggc aatgttcttt cctttaaacc
    30361 tcaatctccg ccatgtcgcc tttctcttgc aaccagttgc ggcgatcttc cgagcgtttc
    30421 ttcgccagca gcatatccat catcgcgtca gtacgctgat cgtcttcatc atcgatagtc
    30481 aactgcacca gacggcgagt gttcggatca agcgtggttt cacgcaattg catcgggttc
    30541 atttccccca gacctttaaa acgctggacg ttcggcttgc ctttcttgcg ttttaattgc
    30601 tcaagtacgc cctctttctc ttcttccgtc agcgcgtaat aaacctcttt cccgagatca
    30661 atacggtaga gcggtggcag tgcgacgtaa acgtgaccgt gtttcaccaa cgcgcggaag
    30721 tgttttacga acaaagcgca gagcagcgtg gcaatgtgca gaccatcaga gtccgcatcc
    30781 gcgaggatac agattttgcc ataacgaagc tggctcagat cgtcgctgtt aggatcgata
    30841 ccgatcgcta ccgaaatatc atgcacttcc tgcgaggcca gcacttcgtc ggaagaaact
    30901 tcccaggtgt taaggatctt acctttcagc ggcatgatcg cctgatattc acgatcgcgc
    30961 gcctgcttgg cagatccgcc tgcggagtca ccttccacga ggaacagttc ggtacggtta
    31021 aggtcctgcg cggtacaatc agccagtttg ccaggcagcg ccgggccgct ggtcagcttt
    31081 ttacgcacca cttttttagc cgcacgcata cgacgctggg cgctggaaat cgccatctct
    31141 gccagcaact ccgccgcctg aacgttctgg ttcagccaca ggataaaggc atctttcacc
    31201 acgccagaaa cgaatgccgc gcattgacgc gaagagagac gctctttcgt ctgcccggca
    31261 aactgcggat cctgcatttt caccgacaga acataggcgc agcgatccca gatatcttcc
    31321 gccgacagct tcacgccgcg cggcagaatg ttgcgatatt cacagaactc acgcatcgcg
    31381 tccaacaggc cctgacgcag gccattaaca tgggtaccgc cctgcatcgt tgggatcagg
    31441 ttgacgtagc tttcggtcag cagttcaccg ccttccggca gccacagtag cgcccagtcc
    31501 acagcttcag tatcgccagc gaaattaccg ataaacggtt tttctggcag cgtcggtaaa
    31561 ccgtttaccg cttccgccag gtaatcattc agaccgtcct gatagcacca gcgctgttcg
    31621 gtgttgttga tctcatcttt aaaggtaatc tcaacgccgg ggcacagtac cgctttggct
    31681 ttcagcacat gcgtcaggcg tgaaacagaa aatcgcgggc tgtcaaagaa ggtttcatcc
    31741 ggccagaagt gcacgctggt accggtattg cgtttaccgc aagtgccgac aacctgtaaa
    31801 tcctgcacct tttcgccatt ttcaaaggcg atgttataaa cctgaccatc gcggcgcacg
    31861 ttaacttcaa cgcgcttcga cagggcgtta accaccgaaa tccccacgcc atgcaggccg
    31921 ccagagaact ggtaattttt gttagagaat ttaccgcctg catgcagacg gcaaagaatc
    31981 aattcaaccg ccgggacacc ctcttccggg tgaatatcca ccggcatccc gcgcccatcg
    32041 tcaataactt ctaacgactg gtcagcatgt aagattacat ccacgcgttt tgcgtgaccc
    32101 gccagtgctt catcgacact gttatcaata acttcttgcc ccaaatggtt agggcgagtg
    32161 gtatcggtat acatccccgg acggcggcga accggctcaa gcccggtgag tacctcaatg
    32221 gcatcagcgt tataagtttg cgtcatggtt taagttagca attcgagttg atcgtcagag
    32281 atggtgcaga ccaagaaaat cgacgatcgg gttgaaataa tcttcgaagc ccgtgaatgc
    32341 gtggttgccg ccttctatga cagtctggcg gcaggaagcg tagtacgcca ccgcctggcg
    32401 gtaatccagc acttcatctc ccgtctgttg cagcagccag atcaaatccg gcgcttccag
    32461 cgggtcaatc tgcatgactt tgagatcgta aatatggcgt gactctagca catattgctg
    32521 cccggtgtat gggttctcgt tctgaccgag atagtccgtc agcagttcaa acgggcgcac
    32581 cgccgggttt accaccactg cgggcagcat aaaacattgt gacaaccagg tggcgtaata
    32641 tccccccagt gacgaaccga caatacccag cgaatcgccg ccatgttcca ggacaatgga
    32701 ttccagcagt tctgccgcgt cggaaggata cggcggcaac tgcggaatga tcatctcaac
    32761 gtcagggtga tgttccgcca gccagttttt taacaagctc gcttttgcag agcgcggcga
    32821 gctgttgaaa ccgtgtaaat aaagaagcgt agacatcagt agccttctga agcggtatca
    32881 ggttggaaac gtgtgtccgc caggcgatgc acctcggtgg tcagcgtgcc atcagcatgt
    32941 aactcgagag tacgccagcc gggcgcgata gtatccagcg taaagttgga acagtgcggc
    33001 ttaaactgca cacaggtcga cggcgttgcc agcaggcggc gaccgttcca gtcgagatcc
    33061 agctcctgat gaatatgacc gcacagcaag tatttgacgt gcggaaactt cgccagcacg
    33121 gtatccagtt cgcccgcgtt acgcagactg tgttgatcga gccaactaca acccgcaggt
    33181 agcggatgat gatgcagcag cagcaacgta tggcgttctg gcgcatcggc cagtttacgt
    33241 tccagccact caagctgaaa ctcgctcagc tcaccgtgcg gcacgccaaa cacctggcta
    33301 tccagcaaca ggatttgcca ttgctcacca ataaacaccc gcttcgccgg ggagataccc
    33361 gcatcctgta acgcgctgta catcgcgggc tggaaatcgt ggttgcccgg cagccagacg
    33421 cagggcgcac gaaaacttgc gatgccttca gcgaaatgct gataggccgc agaggattga
    33481 tcctgcgcta aatcacctgt cgcgacaatc aggtcgaatt cgtgctgatg tggacgaatc
    33541 gcctccagca ccgcctggta gctttcccag gtgtttaccc ctaacagagc ttcgtgcttt
    33601 tgtgcaaaca ggtgagtgtc ggtaatttgt aaaatcctga ctctggcctc accagccaga
    33661 ggaagggtta acaggctttc caaatggtgt ccttaggttt cacgatgcta gtaaaccgga
    33721 atcgccatcg ctccatgtgc taaacagtat cgcaaccagt ccgctaaaaa ctgattaatt
    33781 tgatgctttt cgtcgcgttg atgcaacttt ttattaggat aatcataccg cgctttgaag
    33841 cgaaaaatct gctggcttga acacacttca gccaccatcg cgtcatgata cagacgcacc
    33901 gtcattgacg gaaggctcca gtaactgatc gcgggcgcag tctgttctat tgtcaccagg
    33961 gtagtgtatc gggtcgattc cacaatcgtc agccgatatt gtgcgtttgc cacctgatag
    34021 cttacagttt cgccgggtgc gtcattgcgc ggtaacaaac ggcgcaattg tgaaaagttc
    34081 atctcgcaca ggcgcatcat ttcaggaaag tcaggtgtgt aacgcttcat ttatgcccac
    34141 tcatttttta acgcttgatg atgcagctgc agccattgca aagcgatgac cgacgctgcg
    34201 ttgtcgattt tcccctcttc tacccactgg tatgcctgtt cccggcttac cacatgaacg
    34261 cgaatatctt cgttttcatc agccagacca tgaataccgc ttgcggtcgt ggcgtccact
    34321 tcgcccacca taattgacga acgctcactg gtgccccccg ggcttgccag gaaacttaac
    34381 accggtttgg tccgtttgac tatcagtccc gcctcttcaa tcgcttcgcg acgggcaaca
    34441 tcttccacac tttcaccctc ttctatcatc ccggcaacca tctccagtag ccagggggtt
    34501 tcactggtgt cgtatgcggc aatccgaatc tgctcaatca gcacaacttc atcgcgcact
    34561 gggtcaaagg gtagcaagac tgcggcgtga ccgcgctcaa aaatttcccg ccgtacctca
    34621 tgactcattt gcccgttgaa tagacgatga cgaaatctat aaagatctaa tgaaaaaaag
    34681 ccgcgataaa gtgtttctcg tgcaataatt tctacatcgt ttttgccaaa tgtaacgggc
    34741 aggttgtctg gcttaagcat tgttaatgtc ctggcactaa tagtgaatta aatgtgaatt
    34801 tcagcgacgt ttgactgccg tttgagcagt catgtgttaa attgaggcac attaacgccc
    34861 tatggcacgt aacgccaacc ttttgcggta gcggcttctg ctagaatccg caataatttt
    34921 acagtttgat cgcgctaaat actgcttcac cacaaggaat gcaaatgaag aaattgctcc
    34981 ccattcttat cggcctgagc ctttctgggt tcagttcgtt gagccaggcc gagaacctga
    35041 tgcaagttta tcagcaagca cgccttagta acccggaatt gcgtaagtct gccgccgatc
    35101 gtgatgctgc ctttgaaaaa attaatgaag cgcgcagtcc attactgcca cagctaggtt
    35161 taggtgcaga ttacacctat agcaacggct accgcgacgc gaacggcatc aactctaacg
    35221 cgaccagtgc gtccctgcag ttaactcaat ccatttttga tatgtcgaaa tggcgtgcgt
    35281 taacgctgca ggaaaaagca gcagggattc aggacgtcac atatcagacc gatcagcaaa
    35341 ccttgatcct caacaccgcg accgcttatt tcaacgtgtt gaatgctatt gacgttcttt
    35401 cctatacaca ggcacaaaaa gaagcgatct accgtcaatt agatcaaacc acccaacgtt
    35461 ttaacgtggg cctggtagcg atcaccgacg tgcagaacgc ccgcgcgcag tacgataccg
    35521 tgctggcgaa cgaagtgacc gcacgtaata accttgataa cgcggtagag cagctgcgcc
    35581 agatcaccgg taactactat ccggaactgg cggcgctgaa tgtcgaaaac tttaaaaccg
    35641 acaaaccaca gccggttaac gcgctgctga aagaagccga aaaacgcaac ctgtcgctgt
    35701 tacaggcacg cttgagccag gacctggcgc gcgagcaaat tcgccaggcg caggatggtc
    35761 acttaccgac gctggattta acggcttcta ccgggatttc tgacacctct tatagcggtt
    35821 cgaaaacccg tggtgccgct ggtacccagt atgacgacag caatatgggc cagaacaaag
    35881 tgggcctgag cttctcgctg ccgatttatc agggcggaat ggttaactcg caggtgaaac
    35941 aggcccagta caactttgtt ggtgccagcg agcaactgga aagcgcgcat cgtagcgtcg
    36001 tgcaaaccgt acgttcctcc ttcaacaaca ttaatgcatc tatcagtagc attaacgcct
    36061 acaaacaagc cgtagtttcc gctcaaagct cattagacgc gatggaagcg ggctactcgg
    36121 tcggtacgcg taccattgtt gatgtgttgg atgcaaccac cacgctgtac aacgctaagc
    36181 aagagctggc aaatgcgcgt tataactacc tgattaacca gttgaatatt aagtcagctc
    36241 tgggtacgtt gaacgagcag gatctgctgg cactgaacaa tgcgctgagc aaaccggttt
    36301 ccactaatcc ggaaaacgtt gcaccgcaaa cgccggaaca gaatgctatt gctgatggtt
    36361 atgcgcctga tagcccggca ccagtcgttc agcaaacatc cgcacgcact accaccagta
    36421 acggtcataa ccctttccgt aactgacgac gacgacgggg cttcggcccc gtctgaacgt
    36481 aaggcaacgt aaagatacgg gttatctgcc gcattcttcc cccttctcgc ttcaatttcg
    36541 accagccatc ctctattctg atgggtattt accactggtc ccggaagaca aaaatgaaac
    36601 ggacaaaatc catacgccac gcatcgttcc gcaaaaactg gagcgcacgc catctgacac
    36661 cagtcgctct cgcggttgcc actgttttta tgctggctgg ctgtgaaaag agtgatgaaa
    36721 cagtgtctct ctatcaaaat gctgacgact gttcagctgc aaacccaggc aaaagcgccg
    36781 aatgtaccac cgcgtacaac aatgcgctga aagaagccga acgtactgcg ccgaaatacg
    36841 ccacccgtga agactgtgtt gctgaatttg gtgaaggtca gtgccagcag gcaccagccc
    36901 aggctggcat ggcaccagaa aaccaggcgc aggcccagca atccagcggg agtttctgga
    36961 tgccgctgat ggccggttac atgatggggc gtctgatggg cggcggcgcg ggatttgcac
    37021 agcagccgct gttctcctcg aaaaacccag ccagcccggc ttacggtaaa tataccgacg
    37081 cgacgggtaa aaactatggc gcagcccagc caggccgcac catgaccgta ccgaagacag
    37141 ccatggcacc aaaaccggcg accactacta ccgttacccg tggcggtttt ggtgaatctg
    37201 ttgccaaaca aagcactatg cagcgtagcg caaccggcac ctcttctcgt tcaatgggtg
    37261 gctgataccg atggaaagag tcagtattac cgagcgcccg gactggcgcg aaaaagccca
    37321 cgaatacggt ttcaattttc acaccatgta cggcgagccg tactggtgtg aagatgctta
    37381 ctacaagttg accctcgccc aggttgaaaa gctggaagaa gtcaccgccg aactgcacca
    37441 gatgtgcctg aaagtggtgg aaaaagtgat cgccagcgat gagctgatga ccaaattccg
    37501 cattccaaaa cgcacctgga gttttgtgcg tcagtcatgg ctgactcacc agccatcgct
    37561 ttattcgcgt cttgatctgg catgggatgg cactggcgaa cctaaactgc tggaaaataa
    37621 cgccgataca ccaacgtcac tatacgaggc ggcgttcttt cagtggatct ggctggaaga
    37681 tcagcttaac gccggtaact tgccggaggg cagcgaccag tttaacagtc tgcaagaaaa
    37741 gctaatcgat cgctttgtcg agttgcgtga acagtatggc ttccagttgc tgcatctcac
    37801 ctgctgtcgc gacacggtgg aagatcgcgg aaccattcag tatttgcagg actgcgcgac
    37861 ggaagctgaa atcgctaccg agttcctcta cattgacgat atcgggttag gtgaaaaagg
    37921 tcagttcacg gatttacagg atcaggtaat ttccaacctg ttcaagctgt atccatggga
    37981 atttatgttg cgtgagatgt tctccaccaa gctggaggat gcaggcgtac gctggctgga
    38041 accggcgtgg aagagcatta tctccaacaa agcgcttcta ccgctactat gggagatgtt
    38101 cccgaatcac ccgaacctgc tgcccgctta ttttgcggaa gatgatcatc cgcaaatgga
    38161 aaaatatgtg gttaaaccga tcttctcccg tgaaggcgca aacgtgtcga tcattgagaa
    38221 cggcaaaacc attgaagcag cggaaggtcc gtatggcgaa gaagggatga ttgttcagca
    38281 attccacccg ttaccgaaat tcggcgacag ctatatgctg attggtagct ggctggtgaa
    38341 cgatcaacct gccggaattg gcattcgtga agaccgtgcg ttgatcaccc aggatatgtc
    38401 tcggttttat ccacatattt ttattgagta atcaatgatg ccggatggca ttcgccatcc
    38461 ggtaattgtt aacctatctg caccgacagc atactcaggc tgcccatttc tataccatcg
    38521 accggaatgg taattggctc ctgcccatcc cacgcaccta agacatacaa caacggcaga
    38581 tagtgctccg gcgttgggtt cgataacgcg ccaccttcat ggtcaaggta attcaccaga
    38641 ggatgctgct ctaccgcccc ttgccacgtc agattcgctt tcacatactc attaaacgac
    38701 atcgcccaag gatatggtga actatcaccg tgccacttca ctgtacgcag gttatgcacc
    38761 acgttgccac tggcgaccaa cattattcct tcatctcgca gcgctgccag tttgcgcccc
    38821 atttcgaaat gccaggcggc aggtttgcta ctgtcgatac tcaactgcac catcgggata
    38881 tcggcgtcag gatacatctt aatcagcacg ccccaggaac cgtggtcaaa gccccaggct
    38941 tctttatcca gcgtcaccgg gaccgacgct aacagctcaa ccagatgctg tgccagcaca
    39001 ggcgaacccg gagcaggata atgcgtatcg tacagcgcct gcgggaagcc accaaagtca
    39061 tgaatcgtgg gcggcgtctc catcgcggtc actcctgttc cacgggtaaa ccagtgagcc
    39121 gaaaccacca caatcgcttg cgggcgtggc aatgtcatcc ccaacttctg ccagctgcgg
    39181 gtatacaaat tatcttccag cacgttcatc ggactaccgt gacctaaaaa caatgctggc
    39241 atacgtgttg aagacatgat gatatcctta actaaaggtg tcattttgat atcctcacaa
    39301 tacgcttgtt cggcggagta agaacccgga taacaatgat gatgatcatc agttattttg
    39361 acgatctgcc tgaaggtgaa gatttataag gagttgtcga tgtcagtacc tctcattctg
    39421 accatactgg cgggggcagc cacgtttatt ggcgcgtttc tcggcgttct cgggcaaaaa
    39481 ccctcgaacc gcttactggc gttttcgcta ggatttgcgg cggggatcat gttgctcatc
    39541 tcattaatgg aaatgcttcc tgccgcacta gcagctgaag gaatgtcgcc tgtgttgggt
    39601 tatggaatgt ttatcttcgg tctgcttggc tattttggcc tggaccgcat gttgccacat
    39661 gcgcatccgc aggatttaat gcaaaaatcg gtgcagccgt tgccaaaatc gatcaagcgc
    39721 acagccattc tgctcactct cggcatcagt ctgcataact tcccggaagg gattgccacc
    39781 tttgtcacgg cgagcagcaa cctggagctg ggatttggca tcgcactggc cgtcgcgttg
    39841 cacaatatcc ctgaaggtct ggcagtggca ggcccggttt atgcggcaac ggggtctaaa
    39901 cgcaccgcga ttctgtgggc ggggatttct ggactagcag aaattcttgg tggtgtgctg
    39961 gcgtggttaa tcctcggtag catgatttcc ccggtggtca tggcggcaat catggcggcg
    40021 gttgcaggaa ttatggtggc gctctcggtt gatgaattaa tgccgctcgc caaagagatt
    40081 gaccctaata ataaccccag ctatggcgta ctatgtggaa tgtcagtgat gggattcagt
    40141 ttagtgctgc tacaaacggc gggaattggt taaaaacaac atcagaagtt gtccagcaca
    40201 tggacaactt ctgccagctt cacgaaagcc ttattctacc ttttcggctg tctcttctct
    40261 cgtactgttt aagtatttgt tccgcgcttc aagcagtgcg tctctgtcct ggcgaaacgc
    40321 ccgacaataa cgtttcaaat gcaggaaata acccaccaca accagagcaa tcaatacaat
    40381 ccagtaccag gcgataaaca tccttttacc cttaatattt aacaatacta aacatttagc
    40441 gcataaattt cacatatcct ttttcggata tattcatatg gtcgcataat aacggaacaa
    40501 ttatatgatt ttgatcataa tgaaaaagaa tgcctgatat taatgaaatt ttctcataga
    40561 aagagaagcg gaattaacat cttgcatagc agggcaaaat aaaaaccgaa gccagctaca
    40621 acttcggttt cttgattaag gcagtaatta agcagcggtt ttcagctggc tttacgctca
    40681 tgtgcctgac ggtatgccac caggtcttca atagtcacga gcgccatatt gtgtttattg
    40741 gcaaactcaa tgcactctgg tgcacgcgcc atcgtgccat cgtcattagt cagctcacac
    40801 agcacaccag ccggtttaaa gcctgccagc gtcatcaaat caatagtcgc ttcagtatga
    40861 ccgccacgcg tcagtacacc acctgcctgc gcgcgaagtg ggaaaacgtg gcccgggcga
    40921 ttcagatcag aaggttttgc gccatcggca atcgctgcgc gaacggtcgt aatacgatca
    40981 gcggcagaaa caccggtagt cacaccttca gccgcttcaa tggtcacggt aaaaccggtg
    41041 ccataggcgc tggtgttatt ttctaccatc attggcagat caagttgttt acggcgatct
    41101 tcagtgatgc acaggcaaac aataccgcta ccgtggcgaa tggtcagcgc catctgctca
    41161 acggtcatgg tttctgccgg gaagatcata tcgccttcgt tttcacggtc ttcatcatca
    41221 agcaccatta caccgcgtcc ttcacgcagc gcagccagtg cattttcaac acgttcgaaa
    41281 ggcgtaccaa aagaggaaag tagcgtctga ttcatggtaa aaaaacctca ctaaaattat
    41341 ggttaccaga atcagggcag tcttaggagt ggcggcatat agccaaaata acgtgagcgg
    41401 gtccatgccc gacagaatcg ttactctctc ccatccggac tctaaccgtc ggccccggaa
    41461 ttacaccgga tctgctgtcc tttgagttcg cacccaaagc gctcgcgggc tttcaactga
    41521 gttgatttac cgccggtggg gaatttcgcc ccgccctgag aataagcgga ttcactataa
    41581 cgctaatgat tagcggcagc aacgcatagc ttcacataat tctggtttat gacttaccct
    41641 tatcgcacta caatggtact caacacctat cagtacaggg aaactacaat gattgacccg
    41701 aaaaaaattg agcaaatcgc tcgccaggtt cacgaatcaa tgcctaaagg aatcagggag
    41761 ttcggggaag atgtggagaa aaaaatccgc caaaccctac aagctcagct gacgcgcctc
    41821 gaccttgtaa gccgtgaaga gttcgacgtc caaacgcaag tcctgttacg gactcgtgag
    41881 aaactggcgc ggcttgaaca aagaatgagc gaactggaaa atcgcagtac tgagattaaa
    41941 aagcagccag atccagagac tctcccccca acgctgtaaa catttgtctt taatgcactg
    42001 taaattatga attaatcttc taatttacag tgctttctat taccttatcg cttaattcca
    42061 ttcagaatat catcacaaaa atgaatgagt gcaattgaaa atataattgt tatgtatttt
    42121 ttaagtttta
//
LOCUS       DAZPAC010000010        41904 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZPAC010000010 DAZPAC010000000
VERSION     DAZPAC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41904
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.010"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..623
                     /locus_tag="ACHUPM_000444"
     CDS             <1..623
                     /locus_tag="ACHUPM_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0868330.1"
                     /translation="RNMDGNKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDT
                     YNLIKTLDVCHYDSETKVLTSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANI
                     FNKKHKQHMDDRLIHLIRSNAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQK
                     YGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            885..2507
                     /locus_tag="ACHUPM_000445"
     CDS             885..2507
                     /locus_tag="ACHUPM_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0868331.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2697..2810
                     /gene="yhiY"
                     /locus_tag="ACHUPM_000446"
     CDS             2697..2810
                     /gene="yhiY"
                     /locus_tag="ACHUPM_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR0868332.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2873..3940
                     /locus_tag="ACHUPM_000447"
     CDS             2873..3940
                     /locus_tag="ACHUPM_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0868333.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            3937..6672
                     /gene="rbbA"
                     /locus_tag="ACHUPM_000448"
     CDS             3937..6672
                     /gene="rbbA"
                     /locus_tag="ACHUPM_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR0868334.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6672..7796
                     /locus_tag="ACHUPM_000449"
     CDS             6672..7796
                     /locus_tag="ACHUPM_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0868335.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            7869..8142
                     /locus_tag="ACHUPM_000450"
                     /pseudo
     CDS             7869..8142
                     /locus_tag="ACHUPM_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            8139..8498
                     /locus_tag="ACHUPM_000451"
     CDS             8139..8498
                     /locus_tag="ACHUPM_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR0868336.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(8618..9019)
                     /gene="nikR"
                     /locus_tag="ACHUPM_000452"
     CDS             complement(8618..9019)
                     /gene="nikR"
                     /locus_tag="ACHUPM_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR0868337.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(9025..9831)
                     /gene="nikE"
                     /locus_tag="ACHUPM_000453"
     CDS             complement(9025..9831)
                     /gene="nikE"
                     /locus_tag="ACHUPM_000453"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR0868338.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(9828..10592)
                     /gene="nikD"
                     /locus_tag="ACHUPM_000454"
     CDS             complement(9828..10592)
                     /gene="nikD"
                     /locus_tag="ACHUPM_000454"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR0868339.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(10592..11425)
                     /gene="nikC"
                     /locus_tag="ACHUPM_000455"
     CDS             complement(10592..11425)
                     /gene="nikC"
                     /locus_tag="ACHUPM_000455"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR0868340.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(11422..12366)
                     /gene="nikB"
                     /locus_tag="ACHUPM_000456"
     CDS             complement(11422..12366)
                     /gene="nikB"
                     /locus_tag="ACHUPM_000456"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR0868341.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(12366..13940)
                     /gene="nikA"
                     /locus_tag="ACHUPM_000457"
     CDS             complement(12366..13940)
                     /gene="nikA"
                     /locus_tag="ACHUPM_000457"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0868342.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(14051..14638)
                     /gene="acpT"
                     /locus_tag="ACHUPM_000458"
     CDS             complement(14051..14638)
                     /gene="acpT"
                     /locus_tag="ACHUPM_000458"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR0868343.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(14693..15742)
                     /locus_tag="ACHUPM_000459"
     CDS             complement(14693..15742)
                     /locus_tag="ACHUPM_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0868344.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            15874..17091
                     /locus_tag="ACHUPM_000460"
     CDS             15874..17091
                     /locus_tag="ACHUPM_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0868345.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(17095..17652)
                     /gene="dcrB"
                     /locus_tag="ACHUPM_000461"
     CDS             complement(17095..17652)
                     /gene="dcrB"
                     /locus_tag="ACHUPM_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR0868346.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(17725..18390)
                     /locus_tag="ACHUPM_000462"
     CDS             complement(17725..18390)
                     /locus_tag="ACHUPM_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR0868347.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            18611..18856
                     /gene="tusA"
                     /locus_tag="ACHUPM_000463"
     CDS             18611..18856
                     /gene="tusA"
                     /locus_tag="ACHUPM_000463"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR0868348.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(18958..21156)
                     /gene="zntA"
                     /locus_tag="ACHUPM_000464"
     CDS             complement(18958..21156)
                     /gene="zntA"
                     /locus_tag="ACHUPM_000464"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR0868349.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(21230..21856)
                     /locus_tag="ACHUPM_000465"
     CDS             complement(21230..21856)
                     /locus_tag="ACHUPM_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR0868350.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            21997..22356
                     /locus_tag="ACHUPM_000466"
     CDS             21997..22356
                     /locus_tag="ACHUPM_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR0868351.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(22359..22628)
                     /locus_tag="ACHUPM_000467"
     CDS             complement(22359..22628)
                     /locus_tag="ACHUPM_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR0868352.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(22618..23214)
                     /gene="rsmD"
                     /locus_tag="ACHUPM_000468"
     CDS             complement(22618..23214)
                     /gene="rsmD"
                     /locus_tag="ACHUPM_000468"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR0868353.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            23364..24860
                     /gene="ftsY"
                     /locus_tag="ACHUPM_000469"
     CDS             23364..24860
                     /gene="ftsY"
                     /locus_tag="ACHUPM_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR0868354.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            24863..25531
                     /gene="ftsE"
                     /locus_tag="ACHUPM_000470"
     CDS             24863..25531
                     /gene="ftsE"
                     /locus_tag="ACHUPM_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR0868355.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            25524..26582
                     /gene="ftsX"
                     /locus_tag="ACHUPM_000471"
     CDS             25524..26582
                     /gene="ftsX"
                     /locus_tag="ACHUPM_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR0868356.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            26827..27681
                     /gene="rpoH"
                     /locus_tag="ACHUPM_000472"
     CDS             26827..27681
                     /gene="rpoH"
                     /locus_tag="ACHUPM_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR0868357.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            27952..29055
                     /gene="livJ"
                     /locus_tag="ACHUPM_000473"
     CDS             27952..29055
                     /gene="livJ"
                     /locus_tag="ACHUPM_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR0868358.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(29254..29637)
                     /gene="panM"
                     /locus_tag="ACHUPM_000474"
     CDS             complement(29254..29637)
                     /gene="panM"
                     /locus_tag="ACHUPM_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR0868359.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            30061..31170
                     /gene="livK"
                     /locus_tag="ACHUPM_000475"
     CDS             30061..31170
                     /gene="livK"
                     /locus_tag="ACHUPM_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR0868360.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            31218..32144
                     /gene="livH"
                     /locus_tag="ACHUPM_000476"
     CDS             31218..32144
                     /gene="livH"
                     /locus_tag="ACHUPM_000476"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR0868361.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            32141..33418
                     /gene="livM"
                     /locus_tag="ACHUPM_000477"
     CDS             32141..33418
                     /gene="livM"
                     /locus_tag="ACHUPM_000477"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR0868362.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            33415..34182
                     /gene="livG"
                     /locus_tag="ACHUPM_000478"
     CDS             33415..34182
                     /gene="livG"
                     /locus_tag="ACHUPM_000478"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR0868363.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            34184..34897
                     /gene="livF"
                     /locus_tag="ACHUPM_000479"
     CDS             34184..34897
                     /gene="livF"
                     /locus_tag="ACHUPM_000479"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR0868364.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            35181..36245
                     /gene="ugpB"
                     /locus_tag="ACHUPM_000480"
                     /pseudo
     CDS             35181..36245
                     /gene="ugpB"
                     /locus_tag="ACHUPM_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            36343..37230
                     /gene="ugpA"
                     /locus_tag="ACHUPM_000481"
     CDS             36343..37230
                     /gene="ugpA"
                     /locus_tag="ACHUPM_000481"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR0868365.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            37227..38072
                     /gene="ugpE"
                     /locus_tag="ACHUPM_000482"
     CDS             37227..38072
                     /gene="ugpE"
                     /locus_tag="ACHUPM_000482"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR0868366.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            38074..39144
                     /gene="ugpC"
                     /locus_tag="ACHUPM_000483"
     CDS             38074..39144
                     /gene="ugpC"
                     /locus_tag="ACHUPM_000483"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFR0868367.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            39141..39884
                     /gene="ugpQ"
                     /locus_tag="ACHUPM_000484"
     CDS             39141..39884
                     /gene="ugpQ"
                     /locus_tag="ACHUPM_000484"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0868368.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(39871..40311)
                     /locus_tag="ACHUPM_000485"
     CDS             complement(39871..40311)
                     /locus_tag="ACHUPM_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR0868369.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            40431..>41904
                     /gene="ggt"
                     /locus_tag="ACHUPM_000486"
     CDS             40431..>41904
                     /gene="ggt"
                     /locus_tag="ACHUPM_000486"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR0868370.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT         9909 a        10676 c        11579 g         9740 t
ORIGIN      
        1 tgcgaaatat ggatggtaat aaagatagcc gtattaataa aaatgtcatc gttctggctg
       61 gaaatcatga aatcaatttt aatggtaact atactgcacg actagaaaat tataaactct
      121 ctgccggtga tacgtataac ttaattaaaa cgctcgatgt ttgtcattat gactcagaaa
      181 cgaaagtttt aaccagtcat cacggaataa taagaaatga agaaaatgaa tgttattgtc
      241 tcggtgcttt gcaggttcca tttaatcaga tgaaagaccc aattgatcct gaggaattag
      301 caaatatatt taataaaaaa cataaacaac acatggatga tcgtcttatc catctaatta
      361 gaagcaatgc tgtagcatcc acacctgtat atgaaaatta ttttaataac actactgctt
      421 tcagaccaaa acctgaagat atcttcaaat gtggacagac gctcaaaaaa actaaacaga
      481 aatatggcca ttatggatta ggggtagatc agcatcaaaa aattgataat tataccatgg
      541 ggctaaattc ctggaagata gcacctaacg aacgtggtga taaaaaaggt gtcccagggc
      601 ttagttgttt tcaacctcaa taaaacttca tgagtgctgt ggtatctctt tttaattttg
      661 ccagtatttc ataggttttc ttaatcgatt gtagaggcat gttctgatga cgcctctaca
      721 atctttaggg actttctctg ctatgagata agaaatttca tattatgctt taaggctgaa
      781 tgatttgtgc aaaatataat tattttccca tcgaacatga ggtgtatgtc ataagattaa
      841 atgttaacta tgtattcctc gcggtaaata atatgaggta aaagatgaaa attggaactg
      901 tggcaggcac taacgacagt acaacgacaa tcacaacaaa tgatatggtg caagagcatg
      961 ttaccaactt cactaaagaa ctgtttgggt atattgcaaa tggtataggt gatgatatat
     1021 caagtattgc caggacaatg ctcggtgagg ttgtggaaaa aatagatgat tggcaaatag
     1081 aacggttcca acaatctatt caagatgaca aaatttcatt taccatacaa acgaatcatt
     1141 cagaaaaata tagtatgcta tcgggcatgc gcgctcatat tctacgtagg aataactgtt
     1201 atcagtttat tgtgacaatt aatagtaaaa attacggttg ccctcttgat aataccgata
     1261 ttaattggtg cagcattgtt tatttactta ataatatgac agtaaatgat aatgctaatg
     1321 atgttgcagt tactgagtcg tacaaaccca tctggaactg gaaaatatcg caatacaatg
     1381 tatttgatat taaattcgaa acaatgataa aaccccagtt cgctgacaga acctatttct
     1441 ccaattgttc acctgttgat cccacaagta ccagaccgac ctattttggc gataccgatg
     1501 gctcagtcgg tgcagttttg tacgcgcttt ttgcaacggg acatttggga atcatggctg
     1561 aaggagaaaa tttccttagc cagttgctta atatagaaga tgaagttctc aacgtattgc
     1621 taagggagaa ctttaatgag caacttgaca ctaatgttaa tataataatc agtatcctga
     1681 acagaagaga cattgtttta gaaagtcttc agccttattt ggttattaat aaagacgcag
     1741 tcacgccatg tacgtttctg ggcgaccaga ctggcgatcg atttagtaat atctgtggcg
     1801 ataaatttat catcgattta ttaaaacgca taatgagtat caatgaaaac gttcatgtat
     1861 tagctggaaa tcatgaaacg aattgcaatg gtaactatat gcagaacttc acgcgcatga
     1921 aaccacttga tgaggatacc tactctggga taaaagatta tcctgtatgt ttctatgacc
     1981 ccaaatacaa aataatggca aatcatcatg ggataacttt tgatgatcag cgaaaacgtt
     2041 acattatagg accgataaca gtctctagtg atgaaatgac caatgctctc gatccggtcg
     2101 aattagcggc aattatcaat aaaaagcacc atgctgtaat taatggcaaa aagtttaaaa
     2161 atagccgggc tatctcttgt cgatccttta atcgttattt ttctgtttcg acagattaca
     2221 gacctaaact tgaagcacta ttagcatgct cacaaatgtt aggtataaat caagttgttg
     2281 cacataacgg taatggtgga agagaatgta taggtgaaac ggggacagtt ttaggtctta
     2341 acgcccgtga cagtaaacat gctggaagaa tgttcagcat gcataattgc caaattaacc
     2401 ctggtgcagg gcctgagata accactccct ggaagtctta ccagcatgaa aaaaacagaa
     2461 atggacttat gcctttgatc agaaggcgca caatgttaca gctctgaacc atagacaaca
     2521 tgaatattaa tgttactatc tggttgagac tgttgataaa acgtaaaaag gaatgctctg
     2581 ttcctgcaac tgaacaggcg tgaaacgcta tccaacagga tggataccgt tttgtcagcc
     2641 agttttatag atcacactta tgagtatgcg tgtttcattt acggccagga atgctgatga
     2701 tgacaacatt actacccgtt ttcactaagc cttccccgtt ggcgctcaat gctctgcgcg
     2761 ctggtcgtat ttgtcgtttc cttcttatcc cggatggaag aatccgctga acgtcgttcg
     2821 gcggcagttg taacacgcgt cgtttgcttg cagggattat ggcgggtcga ttatggataa
     2881 gagtaagcgc catctggcgt ggtgggttgt cgggttactg gtggtggctg ccgtcgtggc
     2941 gtggtggctg ttgcgcccgg cgggtgtgcc ggaaggcttt gctgtcagca atgggcgcat
     3001 tgaagcgacg gaagtggata tcgccagcaa aattgccggg cgtatcgaca ccattctggt
     3061 gaaagaaggc cagtttgttc gcgaaggcga agtgctggcg aagatggata ctcgcgtgtt
     3121 gcaggagcag cgactggaag ccatcgcgca aatcaaagaa gcacaaagtg ccgttgctgc
     3181 cgcgcaggct ttgctggagc aacggcaaag cgaaactcgt gccgcgcagt cgctggttaa
     3241 tcaacgccag tccgaactgg actccgtggc taaacgtcat acgcgttccc gctcgctcgc
     3301 ccaacgaggg gctatttctg cgcaacagct ggatgacgat cgcgccgccg ctgagagcgc
     3361 ccgagctgcg ctggaatcgg caaaagctca ggtatcggct tctaaagcgg ctatagaagc
     3421 ggcccgtacc aatatcattc aggcccaaac tcgcgtcgaa gccgcgcaag ccactgaacg
     3481 gcgtattgcc gcagatatcg atgacagtga actgaaagcc ccgcgtgacg gacgcgtgca
     3541 gtatcgggtt gctgagccag gggaagtgct ggcggcaggc ggtcgggtgc tgaatatggt
     3601 cgatctcagc gacgtctata tgactttctt cctgccaacc gaacaggcgg gcacgctgaa
     3661 acttggcggt gaagcccggc tgatcctcga tgccgcgcca gatctgcgta ttccggcgac
     3721 catcagtttt gtcgccagtg tcgcccagtt cacgccaaaa accgtcgaaa ccagcgatga
     3781 acggctgaaa ctgatgttcc gcgtcaaagc gcgtatccca ccggaattac tccagcagca
     3841 tctggaatat gtcaaaaccg gtttaccggg cgtagcgtgg gtgcgggtga atgaagaact
     3901 ttcgtggcct gacgacctcg tggtgaggtt gccgcaatga cgcatctgga actggttccc
     3961 gtcccgcctg tcgcgcaact ggcgggcgtg agccagcatt atggaaaaaa cgttgcgctg
     4021 aacaatatta ctctcgatat tccggcccgc tgtatggtcg ggctgattgg tccggatggc
     4081 gtcgggaagt cgagcttatt gtcgttgatt tccggtgccc gcgtcattga gcagggcaat
     4141 gtgatggtgc tgggcggcga tatgcgcgac ccgaggcatc gccgcgacgt ctgcccgcgc
     4201 atcgcctgga tgccgcaggg gctgggcaaa aacctctacc acaccttgtc ggtgtatgaa
     4261 aacgtcgatt ttttcgctcg cctgttcggt cacgacaaag cggagcggga agtgcgaatt
     4321 aatgagctgc tgaccagcac ggggttagca ccgtttcgcg atcgtccggc agggaaactc
     4381 tccggcggga tgaagcaaaa acttgggctg tgctgcgcgt taatccacga cccggaactg
     4441 ttgatccttg atgagccaac aacgggggtt gacccgctct cccgctccca gttctgggat
     4501 ctgatcgaca gtattcgcca gcggcagagc aatatgagcg tgctggtcgc caccgcctat
     4561 atggaagagg ccgaacgctt cgactggctg gtagcgatga atgccggaga agtgctggca
     4621 actggcagcg ccgaagagct acggcagcaa acgcaaagcg ctacgctgga agaagcattt
     4681 ataaatctgt taccgcaagc gcaacgccag gcgcatcagg cggtagtgat cccaccgtat
     4741 caacctgaaa acgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg ttttggttcc
     4801 ttcgttgccg ttgatcacgt taatttccgc attccacgcg gggagatttt tggttttctt
     4861 ggttcgaacg gctgcggtaa atccaccacc atgaaaatgc tcaccggact gctgcccgcc
     4921 agcgaaggtg aggcgtggct gttcgggcaa ccggttgatc caaaagatat cgatacccgc
     4981 cgtcgggtgg gctatatgtc gcaggcgttt tcgctctata acgaactcac cgtgcggcaa
     5041 aaccttgagt tacatgcccg tttgtttcac atcccggaag cggaaattcc cgcaagagtg
     5101 gctgaaatga gcgagcgttt taagctcaac gacgttgaag atgttctgcc ggagtcattg
     5161 ccgctcggca ttcgccagcg gctttcgctg gcggtggcgg tgattcatcg cccggagatg
     5221 ttaatcctcg atgagcctac ttctggtgtc gatccggtgg cgagggatat gttctggcag
     5281 ttgatggtcg atctctcgcg ccaggacaaa gtgactatct tcatctctac ccactttatg
     5341 aacgaagcgg aacgttgcga ccgcatctca ctgatgcacg ccggaaaagt gcttgccagc
     5401 ggtacaccgc aggaactggt tgagaaacgc ggagccgcca gtctggaaga ggcatttatc
     5461 gcctatttgc aggaagcggc agggcagagc aacgaagccg aagcgccgcc cgtggtacac
     5521 gacaccaccc acgcgccgcg tcagggattt agcctgcgcc gtctgtttag ctacagccgc
     5581 cgcgaagcgc tggaactgcg acgcgatcca gtacgttcga cgctggcgct gatgggaacg
     5641 gtgatcctga tgctgataat gggttacggc atcagtatgg atgtggaaaa cctgcgcttt
     5701 gcggtgctcg accgcgacca gaccgtcagt agccaggcgt ggacactcaa cctctccggt
     5761 tcccgttact ttatcgaaca gccgccgctc actagttatg acgagcttga tcgtcggatg
     5821 cgtgcgggcg atatcacggt ggcgattgag atcccgccca atttcgggcg cgatatcgcg
     5881 cgtggtacgc ctgtggaact cggcgtctgg atcgacggag cgatgcccag tcgtgccgaa
     5941 acggtaaaag gttacgtgca ggcaatgcac cagagttggc ttcaggatgt ggcgagccga
     6001 caatcgacac ccgccagcca aagcgggctg atgaacattg agacgcgcta tcgctataac
     6061 ccggacgtaa aaagcctgcc agcgattgtt ccggcggtga tcccgcttct gctgatgatg
     6121 atcccgtcaa tgctaagcgc ccttagcgtg gtgcgggaaa aagagctagg gtcgattatc
     6181 aacctttacg tgacccccac cacccgtagc gaatttttgc ttggcaaaca gctgccgtac
     6241 atcgcgctgg ggatgctgaa ctttttcctg ctctgcggcc tgtcggtgtt tgtgtttggc
     6301 gtaccgcata aaggcagttt cctgacgctc accctggcgg cgctgctgta tatcatcatt
     6361 gccaccggaa tggggctgct gatctccacc tttatgaaaa gccagattgc cgccattttc
     6421 ggaacggcga ttatcacgtt gatcccggcg acacagtttt ccgggatgat cgatccggta
     6481 gcttcgctgg aagggcctgg acgttggatc ggcgaggttt acccgaccag tcattttctg
     6541 actatcgccc gcggaacgtt ctcgaaagcg ctggatctga ctgatttgtg gcaacttttt
     6601 atcccgttgc tgatagccat cccgctggtg atgggcttaa gcatcctgct gctgaaaaaa
     6661 caggagggat gatgcgccat ttacgcaata tttttaatct gggtatcaaa gagttgcgca
     6721 gtctgctcgg tgataaagcg atgctgacgc tgattgtctt ctcgtttacg gtgtcggtgt
     6781 attcgtcagc gaccgttacg ccaggatcgt tgaacctcgc gccgatcgcc attgccgata
     6841 tggatcaatc gcagttatcg aaccggatcg ttaacagctt ctatcgtccg tggtttttgc
     6901 caccggagat gatcaccgcc gatgagatgg atgccggact ggacgccgga cgctatacct
     6961 tcgcgataaa tattccgcct aattttcagc gtgatgtcct cgccggacgc cagccggata
     7021 ttcaggtgaa cgtcgatgcc acgcgcatga gccaggcatt taccggcaat gggtatatcc
     7081 agaatattat caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat aacagcgaac
     7141 cgttggtatc gctggaaacc cggatgcgct ttaacccgaa cctcgatccc gcgtggtttg
     7201 gcggggtgat ggcgatcatc aacaacatta ccatgctggc gattgtattg accggatcgg
     7261 cgctgatccg cgagcgtgaa cacggcacgg tggaacactt actggtgatg ccgataacgc
     7321 cgtttgagat catgatggcg aagatctggt cgatggggct ggtggtgctg gtggtatcgg
     7381 gattatcgct ggtgctgatg gcgaaaggtg tactgggcgt accgattgaa ggctcgatcc
     7441 cgctgtttat gctgggcgtg gcgctcagtc tgtttgccac cacgtcaatc ggcattttta
     7501 tggggacgat agcgcgttca atgccgcaac tggggctgct ggtgattctg gtgctgctgc
     7561 cgctgcaaat gctttccggt ggatccacgc cgcgcgaaag tatgccgcag atggtgcagg
     7621 acattatgct gaccatgccg acgacacact ttgttagcct cgcgcaggcc attctctacc
     7681 ggggtgccgg attcgaaatc gtctggccgc agttcctgac gctgatggca attggcggcg
     7741 cgtttttcac cattgcgctg ctgcgattca ggaagacgat tgggacaatg gcgtaaggaa
     7801 gggaaacttg acttcatact gtgatgtcat actattgatg tcataaagtg aagtcataag
     7861 gacatgccat gagaaccaag gtacaggctt tacggaagaa aaaaaaatac attggatcaa
     7921 atatttaaaa ctcctgttcc tcaagggatc aagtggtctg atatagagtc actggttaaa
     7981 gcattaggcg gagaaattaa ggaaggaaga ggttcgcgtt gtaagttcat actaaatatg
     8041 agcgttgcgt gtttccatcg gcctcatccg tcgccagata ccgataaagg cgctgtagaa
     8101 agcgtgcgtg actggttact aagtataggg gtaaaaccat gatcaaactg aagacgccaa
     8161 actctatgga gattgccggg caaccagctg ttattaccta cgtgccagaa ctgaatgcat
     8221 ttcgtggcaa attcctgggc ttgtcaggtt attgtgattt tgtctctgac agcattcagg
     8281 ggctgcaaaa agaaggggaa ctttccctgc gagagtatct tgaggattgt aaagctgcgg
     8341 gtattgagcc gtatgcgcgt acagaaaaga ttaagacctt tacgttacgc tatccagaat
     8401 cgctgagtga acgccttaat aatgcggctg cgcagcaaca agtctctgtt aatacctata
     8461 ttattgaaac actgaatgag cgtttgaatc atttataaat tttagcggcg tacattacgc
     8521 agaatatctg cctttacagg atctatctat gcaactgaac ttaattttat aaattaagtt
     8581 cagttaatat cttttctttc ctcgtttcag aaataactca atcttccttc ggcaagcact
     8641 gcaaatgccc atgccgcacg ccgcgctggg cgataacgtc atcggcaaaa tgctgcacgt
     8701 cgcccatatc acctttcagc acggcgattt ccagacagtc gtcgtgattg atatgcacat
     8761 gcagcgtggc gacggagaga tcgtggtgat ggtgctgggt ggagacaatg cggctggcta
     8821 agtcgcgttt ttcgtgttca tacacatagg agagcaccgc gaaaccttgc gtgccgtgct
     8881 gctgagtggc ctcctgggcc agggcgctac gcagaatatc gcggatagct tcggaacggt
     8941 tgttataacc acgacgctgg ctcaggctgt ccagcgtctc cagtaaatcg tcatcaagcg
     9001 tgatggtgac tcgttgcatt tgcgttaaac cttttctgtg gtgcgacggc gcacggggaa
     9061 tgcgggtaat accgcgtttt gtagcacacg tccggcgtca gaggaaaagg ttaatttatc
     9121 tcccaccacc tgggtttcga cgatttgtcc gttgtccatc accatcaccc gctgacagaa
     9181 gcgttccacc aggcgtaagt cgtgggtgat gaacaggcag gcggtgccaa actgttgttg
     9241 tagctttttc agcaggcgaa tgacgcccgc ctgtaacacg agatcaaggt tagaaacggc
     9301 ttcatccaga atcagtagtt tcggttcgac cgccagcgcg cgagccaggc agacgcgctg
     9361 gagctggccg ccgcttaact gcggtgggcg tttgtcgaga acgctgtcat cgagatcgac
     9421 cgccttcagc atttcgctgg cgcgcgccag ttgttcggat tttttcagtg atagcaggtg
     9481 gcgcatcggt tcacgcagga tctcgcgcac ggttttgcgc ggattcacgg cgctgatgga
     9541 gtcctgaaat accatctgaa tatcgcggcg gaacgcttta cgctgggcgc gattgagttt
     9601 cgccagcggt tcgccacgcc agctaatatt cccctgcgcg ggcgattcta aacccaccag
     9661 caaccgcgcg agggtacttt tcccgcagcc gctgcgcccc agcagggcga cggtttcgcc
     9721 gcttttcagg gtcagggaaa cgttattcag caccgcctga tgttgatgtt ttccgttaaa
     9781 tccaccgtgg gcatagtgat gggaaaggcc ggagacgtta agtaaagtca tgatgccagc
     9841 tccataccgt agagggcgag atgagcggaa accaggctgc gcgttaccgc atgtttgggg
     9901 gcgttaaaca gcgtttctac atcgccctgt tcgacaattt taccttgtga catcaccgcc
     9961 acgtcatccg ccagacgcgc cacaacgccc atatcatggg tgaccagcag cattcccggc
    10021 gcttgttttt gcataatgct ttccagcaga tcgaggatgc gcgcctgtgc taccacgtcg
    10081 aggtcggtgg tcggttcatc ggcgatgata aacggtgatt cacacagcac cgccatcgca
    10141 atcatcatgc gctgcaacat gccgccgctc atctcgaacg ggtacagctt cagcacgcgc
    10201 gcggcgtttt ccagccccac cgcttctatg gcagcggtaa gcgtagcgtc atcggcgggt
    10261 ttccctaacg ccaggcaggt ttcccgcgcg tgggtgtgca tggtgtgtag cggattaaag
    10321 gcgctgcgcg ggttctgcat gatggtggca attttgatgc cgcgcagggc gcagggataa
    10381 accgctttgc catcggctaa aatttccccc gccgtctggc gaacgccagc gggcaaaatg
    10441 cccagcgtcg cggcgcaggt taatgatttc ccgctgccgc taccgccgac taacgccagc
    10501 acgcgcccgc gttgcagggt taacgataca ccgtgtacca gcggctgcgc ggcctgtagc
    10561 gcgatattac gtagttcaat ctgttgcggc attagtgtgc gtgctccgtc accagatgag
    10621 gatccagatg atcgcgcagt gcgtcaccca ccaggttaaa ggccatcacg ctgataaaca
    10681 gcgccagccc cggccagaac atttgcagcg gctgggtcca gatatactgg cgcgcgtcgt
    10741 taatcatcac gccccattcg gcggtcggcg cggtcacacc gaggccaagg aaagacatcc
    10801 ccgcgacgtg cagcatcata tggccgatat ccagcgttgc cagcaccagc agcgaaggga
    10861 tcaccgcgcc tgccagatga tcgacaaaca cccgcacatg gcccgcaccg gaaagccgtg
    10921 acgccagcac aaactcgcgt tggcgtagtg aaatcaccaa gctgcgcacc atgcgtgcat
    10981 accacgccca gtgcgacagg gcgatggcga taattacgtt ggtcagcccg gtgccgagca
    11041 cgccaaccat aaagaacgac agaatcgagg tcgggaaggt cataaacata tcggcgacgc
    11101 gcatggtggc ctgatcaacg cgcccgccaa tcaacccggc gctgccgcca ataaccagcc
    11161 ctaatgtcag caccagcagc aggcaggcca ttaccgaacc gagcgacacg cgggtcgctg
    11221 ccatcagccg cgagaaaata tcgcgaccta agtgatcggt gcccagccag tgctgcgcat
    11281 ccggcgaaag caggcgcgac ggcaaatcaa tcgcctgggg gtcatacggc agccaccact
    11341 ggctggtgag cgcaatcagc gccagcaggg cgataatgat cagcgccagg cgtaccgacc
    11401 agcgggaaga gaggaaaaag ttcacgcgtg cgctccttca tgacgacgaa tgcgcgggtc
    11461 cagcgcggcg ttgagcaaat cgacaatcaa attacagacc acaaaaacca ccaccatcat
    11521 cagcgtaaag cactgaatca ccggatagtc acggttaaaa atcgccgaca ccgcatagcg
    11581 cccgacgccc ggccaggcaa agatgttttc gataatcatc gtcccgccaa tcagttcgcc
    11641 gatgtgcatc cccacggcgg tgatcatcgg cagcgaggca ttgcgcagaa tatggcggcg
    11701 ttcggtctgt ttgtcgttca ggccgcgcag acgcgcccaa gtgacgtgac gctgaccggc
    11761 gacgtccagc atactggcgc gcagcagtcg cgcgttaatc gccagcgaca taaaggcaat
    11821 ggaaaccgca ggcaaaatga tgtgctgcca gccgccgtaa cccatcgcgg gtagccattg
    11881 cagatacacc gaaaacgcca tcaccagtaa aaacgccagc cagaagttag gcatcgacac
    11941 gccaagaaac gcgatgaaac gtacggcgaa atccggcaga cggtcgcgat ggcgcgccgc
    12001 ccagataccg agcggtacgg aagtgagcag aattaatacc agcgccgcac ctgcaagttc
    12061 cagcgtggcg ggcaggaagt tcagcatatc gtccagcacc gggcgttggc tggcgaatga
    12121 gataccaaag tcaagatgca gcgccttcca caaccaggta ccgtactgga cgtacagcgg
    12181 ctgatccagc ccgagcatgg tgcgggtaga ggccagcatc tccggcgtcg gcggcaggtt
    12241 agataaacgc agataatcga gcgccgggtc gccggtaccg aggcgcagca tcagaaaaat
    12301 gatcaccgag gcggcgagca ccatcgggat cagcagcaga aagcggcgta atacgtaacg
    12361 caacattaag gtttcaccgg tttaatctgt tcgaacggaa tttcggtggc gatcggcgcg
    12421 taggggatat tacccagctc cggttttgat accaccatca tcgagatgta gctgataggc
    12481 agataaaccg cctcgtcatg cagacgggtc aaaatgtcgc gatacagcgc ctgacgttgc
    12541 gtttcgtcat gggtcgccag cacttcgccg atctctttat caatcagcgg cttgtcggct
    12601 aatccttgct gtgcctggaa gtcagcgtgc gacggtacgc gcattgaact caggaaggcg
    12661 tgtggatcgt atggcgcgcc ccaggtgcgg tggaaaatca tgccaaaacg accgtcgcgc
    12721 tgacgagcat agatactgct ctcttcttcg ccaatcagcg agacatctgc gccaatctgg
    12781 cgcatatcag cctgaatgat ttccgccatc gatttgctta acgcatcggt gccgatgaac
    12841 gacagttcaa tgcgcagcgg ctgaccattt ttctcgcgga tgtctttgcc cgcaggcagc
    12901 gtccaaccgg ctttttccag caactctttc gctttctgcg gatcgtactg gcgcggtttc
    12961 aggccgagat tagcgtaagg cacagaaggg gcaaacaggg tgtcggcgac ctgcggggtg
    13021 ccatataacg cgttatcaat cagcgatttt ttgtttaccg cgtaattaag agcttcacgt
    13081 actgccagct cgttggtggg ggctttggcg gtattgagcg ccagcatcac ggtttcgatc
    13141 ggctgtgaca gttgggtgtg gtaagccgga ttctggctaa agcgggcgaa ggtatcgagc
    13201 ggtaataacc cttcgtttcc gtacagcagg tcgatatcgc cagtttcaaa cgccaccgcg
    13261 cgggtagtcg ggtccgggat gacgttaaag gtgatctttt taatcgctgg cttttcgccc
    13321 cagtagtttt cgttacggac gaagacatcg tactgattca gtttcgattc ctgcaaaatc
    13381 cacggtccgg tgccaatcgg cgctttaatt ccgttcatgg tttcatggtt tttaaactgc
    13441 gagggagcga taaaacggaa aggacggggc agggccagtt cttgcaggaa aggatagtag
    13501 gcgcttttca gggtaatttg cagctctgtt ttactgagtg ctttaacatc aacaatctgg
    13561 tttgccagct ccagccaggc gtgacgttga cggttatcga gcactgcgcg gaagttttct
    13621 gccgccgcct cggcatcgaa cggttcaccg ttggagaatt tcacgtcatc acgcagggtg
    13681 aaggtccagg ttttaccatc ttctgaatga gtccagcttt ttgccagcca cgggatcacc
    13741 gaaccgtctg cctgatattt caccaatggt tcataaacca tgctctgggc gaacatctgg
    13801 ttaggcgtgt aaaggtgcgg gtttagtggc ccgacattca ccggccaggc ggtagtgatt
    13861 tcatctggtg cagcggcatg gacgataaaa gacgcacaag ccagcagcgc aaatagagtg
    13921 cggcggagtg tggagagcat ggtaacccca atggattaaa atagatggcg gaaataagta
    13981 tgacgatttt aagtattcgt catactgatt acctgttctg gatcaataga tgggcagtcg
    14041 ggtgggcgga tcagttaact gaatcgatcc attgcacact gtcggcggtg agcgtaaagg
    14101 gggtgggggt gcagatcgca aggcttaaat tttctagctg acaatggctg actgacagcg
    14161 aggagtgata ggtgctatct acgctgacga tttgccaggc gctgccgcca cgctgtttaa
    14221 cgatggcttc tttgcgcgtc cagatgcgcc agaacatttc cagttgctga tcaggatgca
    14281 cggcgtccat ttcagcgtgt tccccgaggc tgaacacggc gttcgccagc cagcgccagt
    14341 tggcgcgcgg gcgaatcact tcgatatcgc agccgacttc gccttcatcg ctcaacagca
    14401 gagcgatatc gtcaccgcta tggcttaagt tgaaccatag cggcgtttcc ggcgcgaatg
    14461 caggtttgcc ttgttcgcca tagatgatct ccggtagcgg ggaaagcgtg tgcgaaagca
    14521 atgcacgccc cgccagccag cgttcgcgtc gtggaccttg cggtgcttgc tcgcgtaaac
    14581 ccggtggcag tggagctgcg cttaaggtcg aaactttccc cagaactatc cgatacatat
    14641 cagggccaac gtttaatgga aaatgaaagt gcgtatcgta tcacttgtcg cctcatcccg
    14701 gtaaccgact tttcggtctg cctggcccca gtaaaatcgc cagtttgcta ccgcctttgg
    14761 tggtttccat ccagatttta cacacactgg ttaatggcac cgaaagtagc atccctaccg
    14821 ggccgagcag ccatccccaa attaacaatg aaagaaatac caccatggtg gacatcccca
    14881 ggcgatggcc catcatccgt ggttctaaaa tattgccgat gaccatatgg accactaaaa
    14941 acaatgcgcc gaccagaata cattcgtaaa caccattaaa cagcagcacc tgaatcattg
    15001 gcggtacggc ggaaattacc gcgccgatat tgggcacgta gttgagcaaa aacgccagta
    15061 ctgcccacat cagcgcaaac tgcaccccca tcagctccag ccccagccag acgatgacac
    15121 ctgtccataa actgagtagc gtcttcaatg caagatagtg cgaaacgcct ttaagtgcgc
    15181 ggtgtaatcc tgcgatgtga atctgtgggt tattcagcgc aaaacgcatt ttgtaaggga
    15241 cgtggcgcac ttcaaacagc ataaaaacta cggtcatcac cagcaaaagc acgctcgcca
    15301 ttgccccgga aagcccggtc attagcgctg tggtgaaggt aaccactttt tccgagtcca
    15361 tccgctgcag cattcgctcc ggcgacatat gcaaattaag aaaaggcaac atctcctgca
    15421 atttaaaaag tttgcgcgtc agctccttat taaacttcgg cagcatagag ataaattcgt
    15481 taaacgatgc cgccagtacg ccgaccagcg cggttagtgc gatcagcatc accaccacta
    15541 caatcgtaat ggcaacgggg cgttgtactc cccgacgaat aaaccaggta acgagcgggt
    15601 tgaggacgat ggcaaaaaac agcgccagta acagctgcac aatgatatct gccgctgcgt
    15661 gaatgcccgc gaggatcact accagcgagg ccagcttgag cagaatgtgc atgcccgttt
    15721 tatcgggttg agggatttcc attggggctt ccttgtgact ttttgtatta agtgtagcgg
    15781 gagtcacgcc agcaatattc tgattcttac tgctgatttt cacgtcagca cgtggtaaaa
    15841 atgaaacact gttgtaaaaa tgtggtgatc ctcatgcccg aacccgtagc cgaacccgcg
    15901 ctaaacggat tgcgcctgaa tttgcgcatt gtctccattg tcatgtttaa cttcgccagc
    15961 tacctcacca tcgggttgcc gctcgctgta ttaccgggct atgtccatga tgtgatgggc
    16021 tttagcgctt tctgggcagg attggttatc agcctgcaat atttcgccac cttgctgagc
    16081 cgtcctcatg ccggacgtta cgccgatttg ctgggcccca aaaagattgt cgtcttcggt
    16141 ttatgcggct gctttttgag cggtctggga tatctgacgg caggattaac cgccagtctg
    16201 cccgtcatca gcctgttatt actttgcctg ggacgcgtga tccttgggat tgggcaaagt
    16261 tttgccggaa cgggatcgac cctgtggggc gttggcgtgg ttggctcgct gcatatcggg
    16321 cgagtgattt cgtggaacgg cattgtcaat tacggggcga tggcgatggg tgcgccgtta
    16381 ggcgtcgtgt tttatcactg gggcggcttg caggcgttag cgttaatcat tatgggcgtg
    16441 gcgctggtgg ccattttgtt ggcgatcccg cgtccgacgg taaaagccag taaaggcaaa
    16501 ccactgccgt ttcgcgcggt gcttgggcgc gtctggctgt acggtatggc actggcactg
    16561 gcttctgccg gatttggcgt catcgccacc tttatcacgc tgttttatga cgctaaaggt
    16621 tgggacggtg cggctttcgc gctgacgctg tttagctgtg cgtttgtcgg tacgcgtttg
    16681 ttattcccta acggcattaa ccgtatcggc ggcttaaacg tggcgatgat ttgctttagc
    16741 gttgagataa tcggcctgct actggttggc gtggcgacta tgccgtggat ggcgaaagtc
    16801 ggcgtcttac tggcgggggc agggttttcg ctggtgttcc cggcattggg cgtagtggcg
    16861 gtaaaagcgg ttccgcagca aaatcagggg gcggcgctgg caacttacac cgtatttatg
    16921 gatttatcgc ttggcgtgac cggaccactg gctgggctgg tgatgagctg ggcgggcgta
    16981 ccggtgattt atctggcggc ggcgggactg gtcgcaatcg cgttattact gacgtggcga
    17041 ttaaaaaaac ggcctccgga acacgtccct gaggccgcct catcatctta aaacttactg
    17101 aataaccagc gtattaatga tgttttctgc ggtggtctgc gctttttgct gatcgtcagc
    17161 tggcagagta atttgcatgg tcagcagttg attacccacg ttacccagaa taacggaaga
    17221 gtacgccgtc tggcctttcg cggagataat actgtctaac tgctgcattt tgtgaccttt
    17281 cagctcaatg gctttgttgg ttaccacttg cagctgcgga tcgcggctac gttgctgatc
    17341 ttccagacgc ttcgccaaca ccgccagatc ttctttcgga tcatcgccca tgatgacgat
    17401 gactgctttc tgcccggtgg cgtcggacca gacatgcatg ttattggcct gcgttcccag
    17461 cttaccgctc tggtcggtca tatccgctgg cagcgagaaa cttaacttgc catcaagcag
    17521 gttgacggga ttcccggtag cgttactttc cgcgaccgaa ccctgcgccg tagcgttagt
    17581 gtctttatca tcacaggccg caagccccat aaccagcagg ccaattccga catatttaac
    17641 cagattgcgc attgacttct tcctttcgat aaacggccat aacggctcat tcatccatct
    17701 tatcacaact ctgataacga acctttaact cgcctgcaaa gcgttgattt cggatttatc
    17761 tgccagtctt ttcaacagaa tattgagtaa tacgccatac attggcagga agaaaacgat
    17821 actgattaac actttgaaac agtaatcgac cagcgcgatt tccatccagt gttcagccat
    17881 aaaggcatcc gggctacgcc agaaggcaat gaagaaaaag gccagcgtgt cgctgacgtt
    17941 accgaacagt gtggacgctg tcggtgccag ccaccagcgg cgactctgac gcaggcggtt
    18001 aaaaacgtgc acgtcgagga tctgtcccag cgcgtaggcc atgaaactgg cggtggcgat
    18061 acgggcgaca aacaggttga agtgggcgag tgcgccgaat ccctgccagg aacccatata
    18121 gaatagcgac gagatgacgt aggagatcag caacgccggg atcatcaccg caaagatgat
    18181 acgacgggcc agcggtgcgc caaaaatacg cacggtcagg tcggtagcaa ggaaaataaa
    18241 cgggaagcta aacgcgcccc aggtggtatg gaaacccaaa atggagacgg gaagctgaac
    18301 cagatagtta ctggaggtga tcaccagcag atgaaataac gataaccaga acaacgcctt
    18361 atagcgttga gtttgcgaga aaacgttcat attgtacctt tttgattaac cattggggtg
    18421 agggaaccca atacgtacga cacgtctgtt accttaacat aataacgtgc ttaccctttt
    18481 ttcgagccgc cgcatgatac tgctttacgt tgacaatgca atggttaatt ttcacgcaat
    18541 cgttaacctg gtttgcttac ggactcacag ggcgtaaact agcgccgttt ttttatgtga
    18601 tgagaagaaa atgaccgatc tcttttccag ccctgaccac acactcgacg cacttggcct
    18661 gcgctgcccg gaacccgtaa tgatggtgcg caaaactgtg cgcaatatgc agcctggcga
    18721 aacgttgctg attatcgccg acgatccggc cactacccgc gatattcctg ggttttgtac
    18781 ctttatggaa cacgaactgg ttgcgaaaga gacggatgga ctgccttatc gttatttgat
    18841 tcgtaaaggc ggttgatggg gaaagattgg cttcgatgcc gccttttccc ctcaccctaa
    18901 ccctctcccc agaggggcga ggggaccgat cgcgctcaat gttgcgatcg gtttgcctta
    18961 tctcctgcgc aacaatctta acgcattcgc tgtcaccagc accgtcgcac ccgtatctgc
    19021 cagcaccgcc agccacaggc cggtcatccc taacagcgtg gtgacgagga agatcccttt
    19081 cagccccagc gcaatggtga tgttctggcg gatattggcg tgagtggcgc gtgccagttc
    19141 aatcatttgc accaggccgc gcaggtggtt atgggttaat gctgcatcag cggtttccag
    19201 cgccacgtct gtgccactgc ccattgcaat cccaatggcg gcagctttca tcgctggcgc
    19261 gtcgttaata ccatcaccga ccatcgccag gggcgcatgt tgattcagct tggtcaccgc
    19321 tttgacttta tcttccggca ataggcccgc tttaaactcc agccccagct ccccggcgat
    19381 tgccgccgct gcacgtggat tatcgccggt gaggatcact cctttgaccc ccagcgcgtt
    19441 cagttcactg atggcagttg cagcatcggc gcgcagggta tcctgcaatg caatgatacc
    19501 cagcacgtca tcattacgta ctaccagcac aaccgtttgc ccggcgcttt ccagttcatt
    19561 aatcaaacca gcaaatgcat cagcgggatg tttcccggca gcgcaaatca atacgcgctc
    19621 accgttaacc tgcgcttcaa tgccagaccc gaccagcgcc cgctgtgatt cggcggtggg
    19681 aatggcgagt tcagcaacct gtgcttcgcg tacgatggct tgcgccagtg gatgcgtcgc
    19741 gccttgctcg accgccgccg ccagtgtcag cagttcagat tcactaatac ccgttgccgg
    19801 atgaatcgcg gtaacgcgcg gtttaccgac ggtcagcgta ccggttttat caaacgccac
    19861 ctgagtaaca cgacctagtt gttccagcgc cgctccgcct ttaatcaacg ccccacgacg
    19921 cgctgccgcc gccagcccgg aggtaatcgc cgcaggcgtt gagataacta acgcacacgg
    19981 gcagccaatc agcagcagcg tcagcccttt ataaatccac tcctgccagc tggcggcaaa
    20041 cagcagcggt ggcaccagcg tcaccagcag agcgacggcc ataatcgcgg gcgtatagat
    20101 acggctgaaa cggtcgataa accgctcaat gggggcgcga cgctcttcgg cttcttcgat
    20161 cagtttcaga atccggtcaa tggcgctggc acccggttct gacagcactt ccagcgttac
    20221 cagacggtct acgctggtgg ctcctgcagg aactttatcg cccgtcgcgc gctccaccgg
    20281 aatggattct ccggtcaggg cgctttcatc aaaactggca aacggtgaga gcagtttacc
    20341 gtcggcaggc aaacgcccac ctgcggcgac ttcaatcaca tcgccaggcc gcaggctgtt
    20401 aatcgccacc tcttcccgct caccgttacg caggcgcgtg gcggtttctg gtttcagcgc
    20461 cattaacgcg ctaacgccct gacgcgcgcg gctggcggcc cagccttcca gtcgttcacc
    20521 aatcaaaaac agcagcaaca ccatcgcagc ttcagccgtt gcgccaataa acagtgcacc
    20581 aatagcggct acgctcatta aggtttcaat ggcgaagtag ctgccggatt tgatcagccg
    20641 taatgcctga cgagcaatcg ggtacagccc aaccagcgtg gtcgcgataa acgccagttg
    20701 cccgaacgga tgattgaact gctccagacc ccagctgatt gccatcatca cgattagtgt
    20761 aatcagcggc agattctctt tcaggcgcga tgcttgtggc tcgtcggcgg cctgctcatc
    20821 gcgcagggaa tagcccgctt tttgcaccgc agattcaact tgtgcacgaa tgtcattgtc
    20881 ggcatcaacc accagttttt cggtggcgaa caacacctgc acctgattca cacctgcaag
    20941 ctggcgcacg gcattttcta ccttgcgcgc acaggcggcg cagtccatgc cactgacttt
    21001 ccagctatag cgggtgccgg agacgttttc agagagcgtt ggcgagctgg agcatgcgcc
    21061 gtcgcagcaa cagtcgttgg cgttctgtac cgtggttagc ggtttgaacg cagcaaattg
    21121 aggggctttc ttgccgtgat tgtcaggtgt cgacatggca tactccggtt aagttttttc
    21181 tcattaaccg aaggatacac tctggagtcg actccagagt caagttttat cagagataca
    21241 gtgagcggat gatgaggaag tgaccggcaa agtagcaggc cgcagcaatc gcgttatccg
    21301 cacggaagcg tcggcgatag tggctcccca gccagacaaa gttactgata aacagcaacg
    21361 aagcgccaac aaacgcagag agcgccggag cggtcggacg gaagaaccac agttcacctg
    21421 ccagccacac catcaccagc gtcatgccga taaacgtgca gataggccaa cgatactctt
    21481 ccagccgcgt ccagataatc gccagtaaca gcgcacccag aaccagcaac accagtggca
    21541 gaggccagaa gaaagagagc gtcatctgac tggcgaaata gatggtgtac agcaggtgcg
    21601 agaggaaaaa cgcgccgatg gcgtacatca gacgttgacg tggcaacagg gttagcgcat
    21661 cgcccagcag tgaggcgcac agccctgcca gcaccagata gctaatggcg tcgaacatcg
    21721 gcgcttgcca ggccagtaac agcaggagaa gaagggttaa cggtttaaac acccagcgtt
    21781 gccaggttgg cccacgatac gatgcatcca cagatagcca tgcggaaaga cagacagcga
    21841 taaacgacca aagcatctta gttccttgaa ttcgttattt tttcgtaagc ggaatgccta
    21901 cggtcttgct tccagtttag tggcgagggt ggacggatga caacaccata actggcaggg
    21961 tatgcttatt tccgcatttt ccgatgaggg ataaagatga gcaaaccacc tcttttcttt
    22021 attgttatca ttggcttaat tgtcgtcgcc gcatcgtttc gttttatgca acagcgacgg
    22081 gaaaaagctg ataatgatat ggctccgctc cagcaaaagc tggtggtggt gagcaacaag
    22141 cgggaaaaac cgattaacga tcgccgttcg cgccagcagg aagtgactcc ggcaggcacc
    22201 agtatgcgct acgaggcaag cttcaaaccg caaagcggag gaatggagca gacgtttcgc
    22261 ctcgacgccc agcagtacca cgccctgaca gtgggcgata aaggtacgct gagctataaa
    22321 ggaacgcgct ttgttagctt tgttggcgaa caataaggtt attttttaac tttaaatttc
    22381 ttctgccaga ccagcagttc aaacacgccg aaaaggaaaa tccgtacctt ttcggcggtg
    22441 gtcatctgcg ggccatcttt cggtaaagtg gatttcaata gcgccagctg cataccatgc
    22501 atcagcacgg taaaaatcag cgccacgtta acgaaaatat ttagcgggcg cgggaaggga
    22561 tgcaccagat tgaggattaa aaatccccaa acgcagagca ttaacaaacg accaatatta
    22621 atcagcatca ctttctcctt gtgcttcgcg ttgatacagc cgataagcca cctgacccgc
    22681 cactttttcc cgatgtaacg accagtttgc cggaacggtg ggcagaccgt tttcgacttc
    22741 gctttcgaca taaatcaggg cttcgtcagc cagccagccg ttatcttcca gtaaatttat
    22801 cgtctcttct aacaagccac ggcggaacgg tggatcgaca aacacgatat tatgcggtgt
    22861 gcctttttgc gccaggaatg acatcgcgtt gctgttcacc acgcgtgcat tgcctgcttt
    22921 tagtgtcgcc agattcttaa ttaactgctg agaaactgca cgatccatct caattaacgt
    22981 ggcccccgca gcgtagcgcg ataacgcttc cagccccagc gcgccgctcc cggcgaagca
    23041 atccagacat tgggcgtcaa caatgaccgg agccagccag ttaaacaacg tttcgcgtac
    23101 gcggtcggtg gtggggcgca gacctgggct atcaggaacc gggagtttac ggcctcgcca
    23161 ctgcccgcca ataatgcgga tttggccgct gccggaatga ttcggttttt tcatgataaa
    23221 ttgctcaatc cgccagataa cacatacttg caggcggtga tgtgaattaa gttaagtgat
    23281 agactatttc atcatttttt tagctgctat gtacatagcg ttaacgctgt gccatgaagc
    23341 aacagcgagg agtgtagtcg caaatggcga aagaaaaaaa acgtggcttt ttttcctggc
    23401 tgggctttgg tcaaaaagag cagaccccgg aaaaagagac agaagttcag aatgaacaac
    23461 cggttgtaga agaaatcgtt caggcgcaag agcctgtgaa ggcttcggaa caagccgttg
    23521 aagagcagcc gcaggcgcat actgaagccg aggcggaaac ttttgctgcc gacgttgtgg
    23581 aagtcactga acaggttgtt gaaagtgaaa aagcgcagcc tgaagcggaa gttgttgcac
    23641 agccggaacc ggtcgtagaa gaaacgccgg agccagtggc tatcgaacgt gaagagctgc
    23701 cgttgccgga agacgtcaac gccgaagagg tttcgccaga agagtggcag gctgaagcgg
    23761 aaaccgtaga gattgtcaaa gcggcggaag aagaagcggc taaagaagaa atcaccgacg
    23821 aagagctgga agcacaggcg ctggcagccg aagcagcaga agaggcggtg atggtggttc
    23881 ctccggcaga agaagagcag ccggtggaag aaatcgctca ggagcaggaa aaaccaacca
    23941 aagaaggttt cttcgcgcgc ctgaaacgca gcctgttaaa aaccaaagaa aatctcggtt
    24001 ccggatttat cagcctgttc cgcggtaaaa aaatcgacga tgatctgttt gaggagctgg
    24061 aagagcagct tttgatcgcc gatgtgggcg tggaaaccac acgtaaaatt atcaccaatc
    24121 tgacggaagg cgcatcccgc aagcagcttc gtgatgccga ggcgctctat ggcctgctga
    24181 aagaagagat gggtgagatt ctggcgaaag tcgatgagcc gctgaatgtt gaaggtaaaa
    24241 cgccgttcgt gatcctgatg gtgggcgtca acggtgtggg taaaaccacg acgattggta
    24301 agctggcacg tcagtttgag cagcagggta aatcggtgat gctggcggcg ggcgatactt
    24361 tccgtgcagc agcggttgaa cagcttcagg tctggggtca gcgcaacaat attccggtga
    24421 ttgcccagca taccggggcg gattccgcct ctgttatctt cgacgccatt caggcagcta
    24481 aagcgcgtaa tatcgacgtc ctgattgccg ataccgccgg acgcctgcag aacaaatcgc
    24541 acctgatgga agagttgaag aaaatcgtcc gcgtgatgaa gaaactcgac gttgaagcgc
    24601 cgcatgaagt tatgctgact attgatgcca gcaccgggca gaacgcggta agccaggcca
    24661 aactgttcca cgaagctgtt ggcttaaccg gcatcacgct aacgaaactg gacggcacgg
    24721 cgaaaggcgg ggtaattttc tcggtggctg accagtttgg tatccctatc cgctacattg
    24781 gtgtcggcga acgtattgag gatttgcgtc cgtttaaggc ggacgacttt atagaggcac
    24841 tttttgcccg agaggattaa caatgattcg ctttgaacat gtcagcaagg cttatctcgg
    24901 tgggagacag gcgctgcagg gcgttacgtt ccatatgcag ccgggtgaga tggcgtttct
    24961 gaccggtcat tccggcgcag ggaaaagtac cctcctgaag ctgatctgtg ggattgagcg
    25021 gcccagcgcc gggaaaatct ggtttagcgg ccatgacatc acgcgtctga aaaaccgtga
    25081 agttccgttt ctgcgccgcc agattggcat gattttccag gatcaccatc tactgatgga
    25141 ccgtactgtc tacgataacg tggcgatccc gctgattatc gctggtgcca gcggtgacga
    25201 tattcgtcgc cgggtgtcgg cggcactgga taaagtcggg ctactggaca aagcgaagaa
    25261 cttccctatt cagctttccg gcggtgaaca acagcgtgtt ggcattgccc gcgcggtggt
    25321 gaataagccc gcggtactgc tggcggacga accgaccggt aacctggacg acgcgttgtc
    25381 ggaagggatt ctacgtctgt ttgaagagtt taaccgcgtt ggggtaaccg tattgatggc
    25441 aacgcacgac atcaacctga tttcacggcg ttcccatcgc atgctcaccc tgagcgatgg
    25501 tcacttgcat ggaggcgtgg gccatgaata agcgcgatgc aatcaatcat attcggcagt
    25561 ttggtgggcg tctggatcgc ttccgtaaat cggtcggcgg ctcaggcgac ggcggtcgta
    25621 acgcaccgaa acgcgcgaaa tcctcgccaa aaccggtaaa tcgcaaaacc aacgttttca
    25681 acgaacaggt gcgctatgcc ttccacggcg cattgcagga tctgaaaagc aaaccgttcg
    25741 ccacgttttt aacggtgatg gttatcgcca tctccctgac gctgcccagc gtctgttata
    25801 tggtgtacaa aaacgttaat caggcggcga cgcagtatta tccgtcaccg caaatcactg
    25861 tttacttgca aaaaacgctg gacgatgacg ccgctgcggg cgtggtggca cagttgcagg
    25921 ccgagcaagg cgtggagaaa gtgaactatc tttctcgtga agacgcactg ggcgagttcc
    25981 gtaactggtc tggttttggt ggtgcgctgg atatgctgga agaaaacccg cttccggcag
    26041 tggcggtggt gatcccgaaa ctcgattttc aggggacgga atcactcaat acgctgcgtg
    26101 atcgtatcac gcagattaac ggcattgacg aagtgcggat ggatgacagc tggtttgccc
    26161 gtctggcggc gttgaccggg ctggtcgggc gcgtttcggc gatgatcggc gtgttgatgg
    26221 tggcggcagt gttcctcgtc atcggcaaca gcgtacgtct gagcatcttt gctcgccgtg
    26281 actccatcaa cgtgcagaaa ctgattggtg cgacggatgg tttcatcctt cgcccgttcc
    26341 tgtatggtgg cgcactgctg ggattttctg gcgcattgtt gtcattaatt ttgtcagaaa
    26401 ttctggtgct gcgattgtca tcggcggttg cggaagtggc acaggttttc ggaacgaagt
    26461 ttgatatcaa tgatttatcg ttcgatgaat gcctgctatt gctgctggta tgctcgatga
    26521 ttggctgggt ggcagcgtgg cttgccacgg tacaacattt acgccacttt acgcctgaat
    26581 aataaaagcg tgttatactc tttccctgca atgggttccg tagcagggaa agagatcccg
    26641 ttgtctcttc ccggtatttc atctctatgt cacattttgt gcgtaattta ttcacaagct
    26701 tgcattgaac ttgtggataa aatcactgtc tgataaaaca gtggatgata acctcgttgc
    26761 tcttaagctc tggcacagtt gttgctacca ctgaagcgcc agaagatatc gattgagagg
    26821 atttgaatga ctgacaaaat gcaaagttta gctttagccc cagttggcaa cctggattcc
    26881 tacatccggg cagctaacgc gtggccgatg ttgtcggctg acgaggagcg ggcgctggct
    26941 gaaaagctgc attaccatgg cgatctggaa gcagctaaaa cgctgatcct gtctcacctg
    27001 cggtttgttg ttcatattgc tcgtaattat gcgggctatg gcctgccaca ggcggatttg
    27061 attcaggaag gtaacatcgg cctgatgaaa gcagtgcgcc gtttcaaccc ggaagtgggt
    27121 gtgcgcctgg tctccttcgc cgttcactgg atcaaagcag agatccacga atacgttctg
    27181 cgtaactggc gtatcgtcaa agttgcgacc accaaagcgc agcgcaaact gttcttcaac
    27241 ctgcgtaaaa ccaagcagcg tctgggctgg tttaaccagg atgaagtcga aatggtggcc
    27301 cgtgaactgg gcgtaaccag caaagacgtt cgtgagatgg aatcacgtat ggcggcacag
    27361 gacatgacct ttgacctgtc ttccgacgac gattccgaca gccagccgat ggcaccggtg
    27421 ctctatctgc aggataaatc atctaacttt gccgacggca tcgaagatga taactgggaa
    27481 gagcaggcgg caaaccgtct gaccgacgcg atgcaaggtc tggacgaacg cagccaggac
    27541 atcatccgcg cgcgctggct ggacgaagac aacaagtcca cgttgcagga actggctgac
    27601 cgttacggtg tttccgctga acgtgtacgc cagctggaaa agaacgcgat gaaaaaactg
    27661 cgcgctgcta tagaagcgta atttccgcta ttaagcagag aaccctggat gcgagtccgg
    27721 ggtttttgtt ttttgggcct ctacaataat caatttcccc tccggcaaaa cgccaatccc
    27781 cacgcagatt gttaataaac tgtcaaaata gctattccaa tatcataaaa atcgggtatg
    27841 ttttagcaga gtatgctgct aaagcacggg tagccatgca taaaacgaaa taaagtgctg
    27901 aacaacaaca tcacaacaca cgtaataacc agaagaatgg ggattctcag gatgaacata
    27961 aagggtaaag cgttactggc aggatgtatc gcgctggcat tcagcaatat ggctctggca
    28021 gaagatatta aagtcgcggt cgtgggcgca atgtccggtc cggttgcgca gtacggtgac
    28081 caagagttta ccggcgcaga gcaggcggtt gcggatatca acgctaaagg cggcattaaa
    28141 ggcaacaaac tgcaaatcgt aaaatatgac gatgcctgtg acccgaaaca ggcggttgcg
    28201 gtggcgaaca aagtcgttaa cgacggcatt aaatatgtga ttggtcacct ctgttcttca
    28261 tcaacgcagc ctgcgtctga catctacgaa gacgaaggca ttttaatgat caccccagcg
    28321 gcaaccgcgc cggagctgac cgcccgtggc tatcagctga tcctgcgcac caccggcctg
    28381 gactccgacc aggggccgac ggcggcgaaa tatattcttg agaaagtgaa accgcagcgt
    28441 attgctatcg ttcacgacaa acagcaatac ggcgaaggtc tggcacgagc ggtgcaggac
    28501 ggcctgaaga aaggcaatgc taacgtggtg ttctttgatg gtatcaccgc cggggaaaaa
    28561 gatttctcta cgctggtggc gcgtctgaag aaagagaata tcgacttcgt ttactacggc
    28621 ggttaccacc cggaaatggg gcaaatcctg cgtcaggcac gcgcggcagg gctgaaaact
    28681 cagtttatgg ggccggaagg tgtggctaac gtttcgctgt ctaacattgc gggcgaatca
    28741 gcggaagggc tgctggtgac caagccgaag aactacgatc aggttccggc aaacaaaccc
    28801 attgttgacg cgatcaaagc gaaaaaacag gacccaagcg gcgcattcgt ttggaccacc
    28861 tacgccgcgc tgcaatcttt gcaggcgggc ctgaatcagt ctgacgatcc ggctgaaatc
    28921 gccaaatacc tgaaagcgaa ctccgtggat accgtaatgg gaccgctgac gtgggatgag
    28981 aaaggcgatc tgaaaggctt tgagttcggc gtatttgact ggcacgccaa cggcacggcg
    29041 acagatgcga agtaatcatt aatcggcaac tttgggttgc cgccaaattg ctaatatcga
    29101 gtacgttgct tcatgccgga tgcggcgtaa acgccttatc cggcctataa gatccaaaga
    29161 aatcagtaaa ttgcaacaca cattgtaggc ctgataagcg tagcgcatca ggcaatttct
    29221 cgttggtcat cacttttgaa atcaacttga cgattaacgc ttctcccagc cgccctgttg
    29281 cgccgtaaat cccagtgcct gcataaacgc tgtcatcaca ccgcgatctt ccacgcctgc
    29341 atccgccatc caccagcatg aaacgccagg attgttacgc aaaacctctt ccagcagata
    29401 ttgccccaca ccgcgacgtc gggtgacttc ccgcacacgc agggaatcca gtgctccctc
    29461 ggtgccgctt aaggttaccc gtacggcagc gagcaggcgc tcgttaaaac gcgcggcgta
    29521 gatacggtgg ttatcgtcaa cctgtaaaga ggaaggggaa tactccggcc agatcttttg
    29581 caggtcaatc cggtcctggt cgctaaagtt ttctaatcga atgatggtca gcttcatggg
    29641 taacccgtgt aaatcacaaa agtggaacca gtgtagcgaa ataatttaat cggaggcttt
    29701 ctctttttta tttcttttgg caggtgatta attttttaac agcaataatt acaaagttaa
    29761 aacattagag aatgaaaaat acccagcata atcccctgaa tggtagtgaa ttattccgcc
    29821 ctttgtgccg ttattttatg ctgacaaagg cacttttttc tgtttatcta tcaataaatt
    29881 cagaatatta tctgttctta atcgactgaa aaatagggat tttaatcact gttatcacaa
    29941 aatactgcgc taaaccctta atcagacagg caaaaacagt gcagtataaa aaaagaacag
    30001 tctgatttgt taacacataa aaacaaagca acacaacatc acgaatgggg atttttgact
    30061 atgaaacgga atgcgaaaac tatcatcgca gggatgattg cactggcaat ttcacacacc
    30121 gctatggctg acgatattaa agtcgccgtt gtcggcgcga tgtccggccc gattgcccag
    30181 tggggcgata tggaatttaa cggcgcgcgt caggcgatta aagacattaa tgccaaaggg
    30241 ggaattaagg gcgacaagct ggttggcgtg gaatatgacg acgcctgcga cccgaaacaa
    30301 gccgttgcgg tcgccaacaa aatcgttaat gacggcatta aatacgttat tggtcatctg
    30361 tgttcttctt ctacccaacc tgcatcagat atctacgaag acgaaggtat tctgatgatc
    30421 tcgccgggag cgaccaaccc ggagctgacc caacgcggtt atcaacacat tatgcgtact
    30481 gccgggctgg actcttccca ggggccaacg gcggcaaaat acattcttga gacggtgaag
    30541 ccccagcgca tcgccatcat tcacgacaaa caacagtatg gcgaagggct ggcgcgttcg
    30601 gtgcaggacg ggctgaaagc ggctaacgcc aacgtcgtct tcttcgacgg tattaccgcc
    30661 ggggagaaag atttctccgc gctgatcgcc cgcctgaaaa aagaaaacat cgacttcgtt
    30721 tactacggcg gttactaccc ggaaatgggg cagatgctgc gccaggcccg ttccgttggc
    30781 ctgaaaactc agtttatggg gccggaaggt gtgggtaacg catcattgtc gaatattgct
    30841 ggcgatgctg ccgaaggcat gttggtcact atgccaaaac gctatgacca ggatccggca
    30901 aaccagggca tcgttgatgc gctgaaagca gacaagaaag atccgtccgg gccttatgtc
    30961 tggatcacct acgcggcggt gcaatctctg gcgactgccc ttgagcgtac tggcagcgat
    31021 gagccgctgg cgctggtgaa agatttaaaa gctaacggtg caaacaccgt gattgggccg
    31081 ctgaactggg atgaaaaagg cgatcttaag ggatttgatt ttggtgtctt ccagtggcac
    31141 gccgatggtt catccacggc agccaagtga tcatcccacc gcccgtgaaa tgcgggcggg
    31201 tttagaaagg ttaccttatg tctgagcagt ttttatattt cttgcagcag atgtttaacg
    31261 gcgtcacgct gggcagtacc tacgcgctga tagccatcgg ctacaccatg gtttacggca
    31321 ttatcggcat gatcaacttc gcccacggcg aggtttacat gattggtagc tatgtctcct
    31381 ttatgatcat cgccgcactg atgatgatgg gcattgatac cggctggctg ctggtggcgg
    31441 cgggattcgt cggcgcaatc gtcattgcca gcgcctacgg ctggagtatc gaacgggtag
    31501 cctatcgccc ggtgcgtaac tctaagcgcc tgattgcgct catctcggca atcggcatgt
    31561 ccatcttcct gcaaaactac gttagcctga ccgaaggttc gcgtgacgtg gcgctgccca
    31621 gtctgtttaa cggtcagtgg gtggtggggc atagcgaaaa cttctctgcc tctattacca
    31681 ccatgcaggc agtgatctgg attgttactt tcctcgccat gctggcgctg acgattttca
    31741 ttcgttattc ccgtatgggg cgcgcctgcc gtgcctgcgc ggaagatctg aaaatggcga
    31801 gcctgcttgg catcaacacc gaccgggtga ttgctttgac ctttgttatt ggcgcggcga
    31861 tggcggcagt ggctggcgta ctgctcggtc agttctacgg cgtcattaac ccctatatcg
    31921 gctttatggc cgggatgaaa gcctttaccg ctgcggtgct cggtgggatt ggcagcattc
    31981 caggggcgat gattggcggg ctgatcctgg ggattgcgga ggcgctctct tctgcctatc
    32041 tgagtacgga atataaagat gtggtctcat tcgccctgct gattctggtg ctgctggtga
    32101 tgccgaccgg tattctgggt cgcccggagg tagagaaagt atgaaaccga tgcatattgc
    32161 aatggcactg ctctctgccg cgatgttctt tgtcctcgct ggcgtcttta tgggcgtgca
    32221 actggagctg gatggcacca aactggtggt cgacagcgct tcggatgtcc gttggcagtg
    32281 ggtgtttatc ggcacggcgg tggtcttttt cttccagctt ttgcgacctg ctttccagaa
    32341 agggctaaaa agcgtttccg ggccgaagtt tattctgccc gcgattgatg gctccacggt
    32401 gaagcagaaa ctgttcctcg tggcgctgct ggtgcttgcg gtgtcgtggc cgtttatggt
    32461 gtcacgtggg acggtggata tcgccactct gaccatgatc tacattatcc tcggtctcgg
    32521 gctgaatgtg gttgttggtc tttctggtct gctggtgctg gggtacggcg gtttttacgc
    32581 catcggcgct tacacttttg cgctgctcaa tcactattac ggtctggggt tctggacctg
    32641 tttgccgatt gccggactga tggcagcggc ggcgggcttc ctgcttggtt ttccggtgtt
    32701 gcgtctgcgc ggtgattatc tggcgatcgt taccctcggt ttcggcgaaa ttgtgcgcat
    32761 attgctgctc aataacaccg aaattaccgg cggcccgaac ggtatcagtc agatcccgaa
    32821 accgactttc ttcggccttg agttcagccg taccgcccgt gaaggcggtt gggacacgtt
    32881 cagtaatttc tttggcctga aatacgatcc ctccgatcgc gtcatcttcc tgtatctggt
    32941 ggcgttgctg ctggtggtgc taagcctgtt tgtcattaac cgcctgctgc ggatgccgct
    33001 ggggcgtgcg tgggaagcgt tgcgtgaaga tgagatcgcc tgccgttcgc tgggcttaag
    33061 cccgcgtcgt atcaagctga ctgcctttac catcagtgcc gcgtttgccg gttttgccgg
    33121 aacgctgttt gctgcgcgcc agggctttgt cagcccggaa tccttcacct ttgctgaatc
    33181 ggcgtttgta ctggcgatag tggtgctcgg cggtatggga tcgcagtttg cggtgattct
    33241 ggcggcaatt ttgctggtgg tatcgcgcga attgatgcgt gatttcaacg aatacagcat
    33301 gttaatgctc ggtggtttga tggtgctgat gatgatctgg cgtccgcagg gcttgctgcc
    33361 catgacgcgc ccgcaactga agctgaaaaa cggcgcagcg aaaggagagc aggcatgagt
    33421 cagccattat tatctgtgaa cggcctgatg atgcgcttcg gcggcctgct ggcggtgaac
    33481 aacgtcaatc ttgaactgta cccgcaggag atcgtctcgt taatcggccc taacggtgcc
    33541 ggaaaaacca cggtttttaa ctgtctgacc ggattctaca aacccaccgg cggcaccatt
    33601 ttactgcgca atcagcacct ggaaggttta ccggggcagc aaattgcccg catgggcgtg
    33661 gtgcgcacct tccagcatgt gcgtctgttc cgtgaaatga cggtaattga aaacctgctg
    33721 gtggcgcagc atcaacaact gaaaaccggg ctgttctctg gcctgttgaa aacgccttct
    33781 ttccgtcgcg cccagagcga agcgctcgac cgcgccgcca cctggcttga gcgcattggt
    33841 ttgctggaac acgccaaccg tcaggcgagt aacctggcct atggtgacca acgccgtctg
    33901 gagattgccc gctgcatggt gacgcagccg gagattttaa tgctcgacga accggcggca
    33961 ggtcttaacc caaaagaaac caaagagctg gatgagctga ttgccgaact gcgcaatcat
    34021 cacaacacca ctatcttgtt gattgaacac gatatgaagc tggtgatggg gatttcagac
    34081 cgaatttatg tggtcaatca ggggacgcca ctggcaaacg gtacgccgga gcaaatccgt
    34141 aataacccgg acgtgatccg tgcctattta ggtgaggcat aagatggaaa aagtcatgtt
    34201 gtcctttgac aaagtcagcg cccactacgg caaaatccag gcgctgcatg aggtgagcct
    34261 gcatatcaat cagggcgaga ttgtcaccct cattggcgcg aacggggcgg ggaaaaccac
    34321 cttgctcggc acgttatgcg gcgatccgcg cgctaccagc gggcgaattg tgtttgatga
    34381 taaagacatt accgactggc agacggcgaa aatcatgcgc gaagcggtag cgattgtccc
    34441 ggaagggcgt cgcgtcttct cgcggatgac ggtggaagag aacctggcga tgggcggctt
    34501 ttttgccgaa cgcgaccagt tccaggagcg cataaagtgg gtgtatgaac tgttcccacg
    34561 tctgcatgag cgccgtgttc agcgggcagg caccatgtcc ggcggtgagc aacaaatgct
    34621 ggcgattggt cgcgcgctga tgagcaaccc gcgtttgctg ctacttgatg agccatcgct
    34681 tggtcttgcg ccgattatca tccagcaaat tttcgacacc atcgagcagc tgcgcgagca
    34741 ggggatgact atcttcctcg tcgagcagaa cgccaaccag gcgctaaagc tggcggatcg
    34801 cggctacgtg ctggaaaacg gccatgtggt gctctccgat actggtgatg cactactggc
    34861 gaatgaagcg gtgagaagtg cgtatttagg cgggtaataa cacgttgatt gataaggagt
    34921 caaaagactc ctttgagaca ggtgacaaat gtaaaattgc ctgatgcgct gcgcttatca
    34981 ggcctactgg gtgagttgca atatattgaa tttgcacgat cttgtaggcc ggataaggcg
    35041 tttacgccgc atccggcatg aaacgatgag taatctgtag agtttgattc agaccttcta
    35101 tttttccgct tatccgtgcc ccatctccca ttttccctca cccacgccgt caccgccttg
    35161 tcatctttct gacaccttac ttgcaggttt atgaagttgg caccgccacc atgatggcat
    35221 cgaaagccat taaaccggta tatgacgtgt ttaaagaggc agggattcaa ttcgatgagt
    35281 cgcagtttgt gccgacggtt tccggttact actccgacag caaaacgggc cacttactct
    35341 cccagccatt caacagctcg actcccgttc tctattacaa caaagacgcc ttcaagaaag
    35401 cggggttaga cccggaacag ccgccgaaaa cctggcagga tctggcggac tcttccgcaa
    35461 aactgaaagc ctccggtatg aagtgcggct acgccagcgg ctggcagggc tggatccaac
    35521 tggaaaactt tagcgcctgg aacggtctgc cgtttgccag caaaaacaac ggctttgacg
    35581 gcacggacgc ggtgctggag ttcaacaagc cggagcaggt gaaacacatt gccatgctcg
    35641 aagagatgaa caagaagggc gacttcagct acgtcggtcg taaggatgaa tccaccgaga
    35701 agttctataa cggtgattgc gcgatgacca ccgcctcttc cggttctctc gctaatattc
    35761 gtgagtacgc caaatttaac tatggcgtag gcatgatgcc ttacgacgcc gatgcgaaag
    35821 atgcaccgca aaacgccatt atcggcgggg ccagcctgtg ggtgatgcag ggcaaaaata
    35881 aagaaacgta taccggcgtg gcgaagttcc tcgacttcct cgcgaagcca gaaaacgctg
    35941 ccgagtggca tcagaaaacc ggttatctgc caatcaccaa agcagcgtat gacctgaccc
    36001 gtgagcaggg cttttatgag aaaaacccag gggcggatac cgcgacgcgt cagatgctga
    36061 ataagccgcc gttgccgttc accaaagggc tgcgtctggg caacatgccg cagatccgcg
    36121 tgattgtgga tgaagagctg gagagcgtgt ggaccggtaa gaagacacca cagcaggcac
    36181 tggataccgc cgttgagcgt ggaaatcagt tgctgcgccg ctttgagaaa tcgacgaagt
    36241 cttaatcagt gtaatgtcgg atgcgtttcg cttatctgac ctggcatcgc gtgtaggccg
    36301 gataagcgaa gcgcatccgg cacagttcag gaattaaccg taatgtcatc atcccgtccg
    36361 gtgttccgct cgcgctggct gccttatctg ctggtcgcgc cgcagctcat catcaccgtt
    36421 atctttttta tctggcctgc gggcgaagcg ttgtggtact cgcggcaaag cgtcgatccg
    36481 tttggtttct ccagccagtt tgtcggtctg gataacttcg tcacgctgtt ccatgacagc
    36541 tactatctag actccttctg gacgacgata aaattcagca cctttgtcac cgtcagcggt
    36601 ttgctggtat cgctgttctt tgcggcgctg gtggagtaca tcgtgcgcgg cagccgtttc
    36661 tatcaaacct tgatgctgct gccgtatgcc gtggctcccg ccgttgccgc cgtattgtgg
    36721 atcttcctgt ttaaccccgg tcgcgggctg atcacccatt ttctcgctga gttcggctac
    36781 gactggaacc acgcgcaaaa cagcggtcag gcaatgtttc tggtggtgtt tgcctcggtg
    36841 tggaagcaaa tcagctacaa ctttctgttc ttctatgccg cgctgcactc cattccccgt
    36901 tcgttgattg aagccgcagc tattgacggc gcagggccga ttcgccgctt ctttaagatt
    36961 gctctgccgc ttatcgctcc ggtgagtttc ttcctgctgg tggtgaacct ggtgtacgcc
    37021 ttcttcgaca ccttcccggt gatcgacgcc gccacgtccg gcgggccggt tcaggccacc
    37081 actacgctga tttataagat ctaccgcgaa ggctttaccg gactggatct ggcttcgtct
    37141 gccgcgcagt cggtggtgtt gatgttcctc gtcatcgtgc tgacggtggt gcagttccgc
    37201 tatgttgaaa gcaaggtgcg ttaccaatga ttgagaaccg tccgtggctg acgatattca
    37261 gccataccat gctgatcctc gggatcgcgg tgatcctctt cccgctgtac gtggcgtttg
    37321 tcgcggcgac gctggataaa caggccgtct atgccgcgcc gatgacgctc atccccggca
    37381 cacatctgct ggaaaatatc cacaacatct gggttaacgg ggtaggcacg aatagcgcgc
    37441 cgttctggcg gatgttgctt aacagctttg tgatggcgtt cagcattacg ctcggcaaaa
    37501 ttaccgtctc gatgctctcg gcatttgcca ttgtctggtt tcgttttccg ctacgtaacc
    37561 tcttcttctg gatgattttt atcaccctga tgctgccggt tgaagtacgt atcttcccga
    37621 cggtggaagt catcgccaac ctgaaaatgc tcgacagcta cgccggttta acgctgccgc
    37681 tgatggcctc ggcgaccgcc acgtttctgt tccgccagtt ctttatgacc ctaccggatg
    37741 aattggtgga agccgcgcgg atcgacggcg cgtcaccaat gcgtttcttt tgcgacatcg
    37801 tttttccgtt gtccaaaact aatctggcgg cgctgtttgt gatcaccttt atctacggct
    37861 ggaatcagta tttgtggccg ttgttgatta ttaccgacgt tgatctcggc accaccgtgg
    37921 cagggatcaa agggatgatc gctacaggcg aaggcaccac ggaatggaac tcggtaatgg
    37981 cggcgatgtt gttaacgctt atccctccgg tggtgattgt tttagtgatg cagcgtgcct
    38041 tcgtgcgcgg cctggtcgat agtgagaaat aagatggcag gactgaaatt acaggcagta
    38101 accaaaagct gggatggcaa aacccaggtc attaaaccgc tgacccttga tgtggcggat
    38161 ggcgaattta tcgtgatggt cgggccgtct ggctgcggga aatcgacgct gctgcgcatg
    38221 gttgccgggc tggagcgggt gacagaaggc gatatctgga tcaacgacca gcgcgtgacc
    38281 gaaatggagc cgaaagatcg cgggattgcg atggtgttcc agaattacgc gctttatccg
    38341 catatgagcg tagaggagaa catggcgtgg gggctgaaaa ttcgcggcat gggcaagcag
    38401 caaattgccg agcgcgttaa agaagcggcg cgcattctgg aactggacgg tctgcttaag
    38461 cgtcgtccgc gtgagctatc cggcggtcag cgccagcgtg tggcgatggg ccgcgcgatt
    38521 gtgcgcgatc cggcggtgtt cttgtttgat gagccgctct ctaacctcga tgccaagctg
    38581 cgcgtgcaga tgcgtcttga actgcaacag ttgcaccgtc gcctgaaaac gacttcactc
    38641 tacgttactc acgatcaggt tgaagcgatg acgctcgccc agcgagtaat ggtgatgaac
    38701 ggtggcgttg ccgaacagat tggcacacca gttgaagtct acgaaaagcc cgccagcctg
    38761 tttgtggcga gttttatcgg cagcccggcg atgaacctgc tgacaggccg cgtgaataat
    38821 gaaggcacgc atttcgaact ggacggcggt atcgtgctac cgctaaacgg tggctaccgt
    38881 cagtatgccg ggcgtaaaat gactctcggc attcgcccgg aacatatagc gctaagctcg
    38941 caggcagaag gtggtgtgcc gctggtgatg gacacgctgg agatcctcgg cgcagataac
    39001 ctggcgcacg gacgctgggg cgagcagaag ctggtggtgc gactggcgca tcaggagcgc
    39061 ccgacggcag gcagcacgct gtggctgcat ttggcggaaa atcagctgca tctttttgat
    39121 ggtgaaacag gacaacgagt atgagtaact ggccttatcc ccgcatcgtc gctcatcgtg
    39181 gcggcggtaa gctggccccg gaaaacaccc tggcggcaat cgacgtcggg gcaaaatacg
    39241 gtcataagat gatcgaattt gacgcgaagt tatcgaaaga tggcgagatc ttcctgctcc
    39301 atgacgacaa tctcgaacgc accagcaacg gctggggcgt cgcgggtgaa ctgaactggc
    39361 aggatttact gcgcgtggat gcgggcagtt ggtacagcaa agcgtttaaa ggtgagccgc
    39421 tgccgttgct ttcgcaggtg gcggaacgct gtcgcgaaca cgggatgatg gcgaatatcg
    39481 aaatcaaacc caccactggc actggaccat taacgggcaa aatggtggcg ctggcggcac
    39541 gccaactgtg ggccggtatg acgccgccgc tgctgtcatc gtttgagatt gatgctttag
    39601 aagctgcaca acaggcggca ccggaactgc cgcgtggttt attgcttgat gagtggcgcg
    39661 acgactggcg cgaactgacc gcgcggctgg gctgcgtctc tattcatctc aatcataagt
    39721 tactcgataa agcgcgagtg atgcagttga aagacgccgg attacggatt ctggtttata
    39781 ccgtcaataa accccagcgc gcggcagagt tgctgcgctg gggcgtggat tgcatctgta
    39841 ccgatgcgat tgacgtgatt ggtccgaact ttacggccca atagttttca acggaatgtc
    39901 aggctgcggc gtgctgggct gattcagcat gtcgccgttt ctcctttccg gcagcatatg
    39961 ctgctgattc aacgaactat ccggattacg gttgctgttt aacatcccgc cgttggtgtt
    40021 gggcagcatt tgctgccggg cgggatttcg ctcccccggc tgtgactgca acacccgctg
    40081 agaattattg tttatctggt tttctaaatg ctgctgttgc aactgagttt gcgttttcag
    40141 ttgctgattc agcatccctt tttgctggat ttgctgagtc tgcatctggg tttgcatccg
    40201 ctgctggctg ggtatctgat accccggctg gttagggttg ttcagagtat taatgggctg
    40261 tgcaaagccg acaaacggca ggagtgccgt aagaatcaga agtcgtttca tcgcgtatcc
    40321 tcctctgaag atatccttta agtttactcg cttcccgaca aaacgatgat taattcagag
    40381 ttatatacca ggcttagctg gggttgcccc ttaatctctg gagaataacg atgataaaac
    40441 cgacgttttt acgccgggtg gccattgctg ctctgctctc aggaagttgt tttagcgccg
    40501 ccgccgcgcc tcctgcgccg cccgtctcgt atggtgtgga ggaagatgtc ttccacccgg
    40561 tacgcgcgaa acagggaatg gtagcgtctg tggacgccac tgccactcag gtgggggtgg
    40621 atatcctcaa ggagggcggg aatgccgttg atgccgccgt ggcggtgggc tacgcgctgg
    40681 cggtaacgca tccgcaggca gggaatctgg gcggtggtgg ttttatgtta atccgctcga
    40741 aaaatggcaa taccacggct atcgatttcc gcgaaatggc acccgccaaa gcgacccgcg
    40801 atatgttcct cgatgatcag ggcaacccgg acagcaaaaa atcactcact tcgcatctgg
    40861 cttccggcac accgggtacg gtagcaggtt tctcgctggc gctggataaa tacggcacca
    40921 tgccgctgaa caaagtcgtg cagcccgcgt ttaaactggc acgcgatggt tttatcgtta
    40981 acgacgcgct ggctgacgat ctcaaaacct acggtagcga agtgttgccg aatcacgaaa
    41041 acagtaaagc tatcttctgg aaagagggcg agccgctgaa aaagggcgac acgctggtgc
    41101 aggcgaacct ggcaaagagc ctggagatga ttgctgaaaa cggcccggac gaattctata
    41161 aaggcacgat tgcggaacag atcgcccagg agatgcagaa aaacggtggc ttgatcacta
    41221 aagaagattt agcggcctat aaagcggtcg aacgcactcc gataagcggc gattatcgcg
    41281 ggtatcaggt ttactccatg ccaccgccat cctccggcgg gatccatatc gtacaaatcc
    41341 tcaatattct ggaaaacttc gatatgaaga aatacggctt tggcagcgcc gatgcgatgc
    41401 aaatcatggc agaagcggag aaatacgcct acgccgaccg ctcggaatat cttggcgacc
    41461 cggattttgt caaagtcccg tggcaggcac tgaccaataa agcctatgcc aaatccattg
    41521 ccgatcaaat tgatatcaac aaagcgaagc cgtccagtga gattcgccct ggcaagcttg
    41581 cgccttatga gagtaatcaa actacccatt actctgtagt ggataaagac ggtaacgcgg
    41641 tggcggtgac ctatacgctg aacaccacct tcggtacggg cattgtcgcg ggcgagagcg
    41701 gtattctgct taataatcag atggatgatt tctccgccaa accgggcgta ccgaacattt
    41761 acgggctggt gggcggtgat gccaacgccg tcgggccgaa taaacgtccg ctgtcatcga
    41821 tgtcaccaac cattgtggtg aaagacggta aaacttggct ggttaccggt agtcctggcg
    41881 ggagtcggat tattaccacc gtgc
//
LOCUS       DAZPAC010000011        41367 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZPAC010000011 DAZPAC010000000
VERSION     DAZPAC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.011"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            34..663
                     /locus_tag="ACHUPM_000487"
     CDS             34..663
                     /locus_tag="ACHUPM_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR0868371.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="ACHUPM_000488"
     CDS             complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="ACHUPM_000488"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR0868372.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1933..3021
                     /gene="hcxA"
                     /locus_tag="ACHUPM_000489"
     CDS             1933..3021
                     /gene="hcxA"
                     /locus_tag="ACHUPM_000489"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR0868373.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(3031..3228)
                     /locus_tag="ACHUPM_000490"
     CDS             complement(3031..3228)
                     /locus_tag="ACHUPM_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0868374.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="ACHUPM_000491"
     CDS             complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="ACHUPM_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR0868375.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5696..6109)
                     /gene="entH"
                     /locus_tag="ACHUPM_000492"
     CDS             complement(5696..6109)
                     /gene="entH"
                     /locus_tag="ACHUPM_000492"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR0868376.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(6112..6858)
                     /gene="entA"
                     /locus_tag="ACHUPM_000493"
     CDS             complement(6112..6858)
                     /gene="entA"
                     /locus_tag="ACHUPM_000493"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR0868377.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6858..7715)
                     /gene="entB"
                     /locus_tag="ACHUPM_000494"
     CDS             complement(6858..7715)
                     /gene="entB"
                     /locus_tag="ACHUPM_000494"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR0868378.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7729..9339)
                     /gene="entE"
                     /locus_tag="ACHUPM_000495"
     CDS             complement(7729..9339)
                     /gene="entE"
                     /locus_tag="ACHUPM_000495"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR0868379.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9349..10524)
                     /gene="entC"
                     /locus_tag="ACHUPM_000496"
     CDS             complement(9349..10524)
                     /gene="entC"
                     /locus_tag="ACHUPM_000496"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFR0868380.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            10899..11864
                     /gene="fepB"
                     /locus_tag="ACHUPM_000497"
     CDS             10899..11864
                     /gene="fepB"
                     /locus_tag="ACHUPM_000497"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR0868381.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(11945..13195)
                     /gene="entS"
                     /locus_tag="ACHUPM_000498"
     CDS             complement(11945..13195)
                     /gene="entS"
                     /locus_tag="ACHUPM_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR0868382.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            13306..14310
                     /gene="fepD"
                     /locus_tag="ACHUPM_000499"
     CDS             13306..14310
                     /gene="fepD"
                     /locus_tag="ACHUPM_000499"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR0868383.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            14307..15299
                     /gene="fepG"
                     /locus_tag="ACHUPM_000500"
     CDS             14307..15299
                     /gene="fepG"
                     /locus_tag="ACHUPM_000500"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR0868384.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            15296..16111
                     /gene="fepC"
                     /locus_tag="ACHUPM_000501"
     CDS             15296..16111
                     /gene="fepC"
                     /locus_tag="ACHUPM_000501"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0868385.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(16108..17241)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUPM_000502"
     CDS             complement(16108..17241)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUPM_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR0868386.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(17457..21338)
                     /gene="entF"
                     /locus_tag="ACHUPM_000503"
     CDS             complement(17457..21338)
                     /gene="entF"
                     /locus_tag="ACHUPM_000503"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR0868387.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(21335..21553)
                     /gene="ybdZ"
                     /locus_tag="ACHUPM_000504"
     CDS             complement(21335..21553)
                     /gene="ybdZ"
                     /locus_tag="ACHUPM_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR0868388.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(21556..22758)
                     /gene="fes"
                     /locus_tag="ACHUPM_000505"
     CDS             complement(21556..22758)
                     /gene="fes"
                     /locus_tag="ACHUPM_000505"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR0868389.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            23001..25241
                     /gene="fepA"
                     /locus_tag="ACHUPM_000506"
     CDS             23001..25241
                     /gene="fepA"
                     /locus_tag="ACHUPM_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR0868390.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            25416..26036
                     /gene="entD"
                     /locus_tag="ACHUPM_000507"
     CDS             25416..26036
                     /gene="entD"
                     /locus_tag="ACHUPM_000507"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR0868391.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(26158..26310)
                     /gene="hokE"
                     /locus_tag="ACHUPM_000508"
     CDS             complement(26158..26310)
                     /gene="hokE"
                     /locus_tag="ACHUPM_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0868392.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(26661..26813)
                     /gene="hokE"
                     /locus_tag="ACHUPM_000509"
     CDS             complement(26661..26813)
                     /gene="hokE"
                     /locus_tag="ACHUPM_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0868393.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            26782..27050
                     /locus_tag="ACHUPM_000510"
                     /pseudo
     CDS             26782..27050
                     /locus_tag="ACHUPM_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27253..28371
                     /locus_tag="ACHUPM_000511"
     CDS             27253..28371
                     /locus_tag="ACHUPM_000511"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR0868394.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            28437..28685
                     /locus_tag="ACHUPM_000512"
                     /pseudo
     CDS             28437..28685
                     /locus_tag="ACHUPM_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            28750..29118
                     /locus_tag="ACHUPM_000513"
     CDS             28750..29118
                     /locus_tag="ACHUPM_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0868395.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            29212..29865
                     /gene="nfsB"
                     /locus_tag="ACHUPM_000514"
     CDS             29212..29865
                     /gene="nfsB"
                     /locus_tag="ACHUPM_000514"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR0868396.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            29973..31220
                     /gene="ybdG"
                     /locus_tag="ACHUPM_000515"
     CDS             29973..31220
                     /gene="ybdG"
                     /locus_tag="ACHUPM_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR0868397.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(31288..32664)
                     /gene="pheP"
                     /locus_tag="ACHUPM_000516"
     CDS             complement(31288..32664)
                     /gene="pheP"
                     /locus_tag="ACHUPM_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR0868398.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(32766..35909)
                     /gene="cusA"
                     /locus_tag="ACHUPM_000517"
     CDS             complement(32766..35909)
                     /gene="cusA"
                     /locus_tag="ACHUPM_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR0868399.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(35921..37144)
                     /gene="cusB"
                     /locus_tag="ACHUPM_000518"
     CDS             complement(35921..37144)
                     /gene="cusB"
                     /locus_tag="ACHUPM_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFR0868400.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(37160..37492)
                     /gene="cusF"
                     /locus_tag="ACHUPM_000519"
     CDS             complement(37160..37492)
                     /gene="cusF"
                     /locus_tag="ACHUPM_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR0868401.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(37516..38898)
                     /gene="cusC"
                     /locus_tag="ACHUPM_000520"
     CDS             complement(37516..38898)
                     /gene="cusC"
                     /locus_tag="ACHUPM_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR0868402.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            39055..39738
                     /gene="cusR"
                     /locus_tag="ACHUPM_000521"
     CDS             39055..39738
                     /gene="cusR"
                     /locus_tag="ACHUPM_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR0868403.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            39728..41176
                     /gene="cusS"
                     /locus_tag="ACHUPM_000522"
     CDS             39728..41176
                     /gene="cusS"
                     /locus_tag="ACHUPM_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR0868404.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVSKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         9899 a        11464 c        10578 g         9426 t
ORIGIN      
        1 ttataacgaa cggataaaag gaaaacgtca ggaatggggg atactatgca acaacgatta
       61 acgcaggatt taacgcaatt tctcgccagc ttgccggaag atgatcgcat caaagcgatt
      121 aatgaaatcc gcatggcgat ccatcaggtt agcccctttc gtgaagagcc agtggattgc
      181 gttctgtggg ttaaaaacag ccaacttatg cctaatgatt acaacccaaa caacgtggca
      241 ccacccgaaa aaaaactgct gcaaaaatcc attgaaatag atggttttac ccaacccatt
      301 gtggtcacac atacggataa aaatgccatg gaaatcgtgg atggttttca ccgccatgaa
      361 attggtaaag gctcatcatc attaaaatta cgcttaaaag gttatttgcc ggtgacctgt
      421 ctggaaggta cgcgtaatca acgcatcgcc gcgactattc gtcataatcg cgctcgtgga
      481 cgccatcaaa tcaccgccat gtcggaaatc gtccgtgaac ttagccagtt gggatgggac
      541 gataataaaa ttggcaaaga gctgggaatg gacagcgacg aagtgctgcg cctgaagcaa
      601 attaacggcc tgcaagagct gtttgccgac cgtcaatatt cccgcgcctg gacggttaaa
      661 taactaaagc tggcgcaggc gttcagctgc tgccagcaac gtcgattcct tcttggcaaa
      721 acagagacga atcagtttat gtgggaaggg atcggcgcaa aacaccgaca gcggaatcgc
      781 cgctacgccg tgctcctgcg tcagccactg gcaaaactca acatcatcca gggtagaaac
      841 cgcgctgtaa tccaccagca aaaagtatgt accttcacac ggtaaaatct ccagccggct
      901 ttcatttaag gcatttacca gaatatcgcg cttctggcga taaaagtccg gtaacgcaag
      961 ataatgctca ggttctgcac gtagcatatc agcaagcgcc agctgtgccg gggtattcac
     1021 cgaaaaggtc agatactgat gtaccttgcg aatttcggcg ctgatgggcg ctggcgcaac
     1081 acaataaccc actttccagc cggtcatatg ataggtcttg ccaaatgaag aaaccgccac
     1141 tgcccgctca cgcagctgcg aatgcgccag cacactggca tggccctgtt gtgaaaagtt
     1201 gatgtgctcg tagacttcat cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca
     1261 caaagcggcg aaatcagcct gctgccagac agttgcactg gagttatgcg gagtgttgag
     1321 gatcaccagt ctggtgcgct cgcttaataa tgcggcaaat tcctgccagt caacgcgaaa
     1381 atgcggtggt tgcagtgcca tacgcttcac tattccccca gaaagcgcga tggcgggggc
     1441 gtaactgtca tagctgggat caaaacaaat cacttcatcg ccattgcgca ccagtgcggt
     1501 aatcgccgcg tataacgctt ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc
     1561 tggttgatag ccatacaaac gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac
     1621 gccggtcatg ggcgcgtatt ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg
     1681 taaatagcgc ggaccatcaa aatcaggaaa gccttgcgac aggttaatcg cctggtgttg
     1741 ctgcgccagc gcgctcatct gggtgaaaat agtggtgcca agttgtggaa gtttgctttg
     1801 tggaatcaga gggttatttg tcatttttat tgtacctgac gaagttgcgt gttgaaggca
     1861 ctataacacg atgctagtat ttggcaatca agacgtttag atgtctaaat aaaacaataa
     1921 ggacaacaca acatgcctca caatcctatc cgcgtggtcg tcggcccggc taactacttt
     1981 tcacatccag gaagtttcaa tcacctgaac gattttttca ctgatgaaca actttctcgc
     2041 gcggtgtgga tctacggcaa acgcgccatt gctgcggcgc aaaccaaact tccgccagcg
     2101 tttggacttc caggggcaaa gcatattttg tttcgtggtc attgcagcga aagcgatgta
     2161 cagcaactgg cggctgagtc cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc
     2221 gcactgctcg acaccgcgaa agccctcgcc cgccgtctcg gtctgccatt tgttgccgtt
     2281 ccgacgatcg ccgctacctg cgccgcctgg acaccgctct ccgtctggta taacgatgcc
     2341 ggacaggcgc tgcattatga gattttcgac gacgccaatt ttatggtgct ggtggaacca
     2401 gagattatcc tcaacgcgcc gcaacaatat ctgctggcgg ggatcggtga cacgctggcg
     2461 aaatggtatg aagcggtggt gctggctccg caaccagaaa cgttgccgct aaccgtgcga
     2521 ctggggatca ataatgcgca ggccattcgc gacgtattgt taaacagtag cgaacaggcg
     2581 ctgagcgatc agcaaaatca acagttaacg caatcatttt gcgatgtggt ggatgctatt
     2641 attgctggtg gtgggatggt cggtgatctg ggcgatcgtt ttacgcgtgt ggcggcggct
     2701 catgccgtgc ataacggtct gactgtcctg ccgcaaaccg agaaatttct ccacggcacc
     2761 aaagtcgctt atggcattct ggtacaaagc gccttgctag gtcaggatga tgtgctggcg
     2821 caattaaccg gggcgtatca gcgttttcat ctgccgacca cgctggcgga gctggaaatg
     2881 gatatcaata atcatgcgga gatcgacaaa gtgattaccc acaccctgcg tccggtggaa
     2941 tacattcatt acctgccagt cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaaa
     3001 gtggaatcgt ttaaagcctg acgtgcaaat ttagcagcag cgcgcgccgc cttttccacc
     3061 gtagcgcgcg tcctgccgct cacggaaaaa ctcttcgtag gtcatcggcg tttgatcggg
     3121 atggttaacc cgcatatgtt cgacatagtt gtcgtaatca ggcataccaa tcatcagctt
     3181 cgccgcctga cctaaatatt ttccggcttt tgccagtgaa tcaaacatct tggcctccgc
     3241 taaccatgtt gctgaaagag tgccggatgc ggcgtgaacg ccttatccgg cctacaaatg
     3301 ctatcaacgc gctcggtcag tcccctcgcc ccatcgggga gagggttagg gtgaggagga
     3361 accctcggca ctatgccaac attcgccaac atcccccctc actctgactt tagtgtgcgc
     3421 cttttgcctg cgccacgatc tcctcgacat tttccggcat tggctcatac ggcgtttctt
     3481 tcgccgttgg cttcggatct ttcaatgccg ccagtgccga cttaatcgag aacagcgcca
     3541 gaaccaccac gaccaccata aagaagatgg ttaacccggc atccagacgg ttgttgaaca
     3601 ccagttgcgc cagctgtgac tcagtatact gcgacggaat attgccgctg tctatcattg
     3661 cctggaactt attagcaatg gccaggaagc cgactttcgc atccgggcta aacgctttct
     3721 gccagcctgc ggtcagggta caaatcagca gccaggccgt tggtaccagc gccacccagg
     3781 cgtaacgttg acgtttcatc ttgaacaaca ccacggcaca gagcatcagc gccatccctg
     3841 ccagcatctg gttggcaata ccaaacagcg gccacagagt gttaatgccg cccagcggat
     3901 cgaccacgcc ctgatggagg aagtagcccc acgccagtac gcacagcgct gttgccagca
     3961 ggttagcagg cagtgaatcg gtccgcttca ggccaggaga caccacgccc agcagatcct
     4021 gcaacataaa gcgcgcagca cgcgtacccg catctaccgc cgtcagaata aacagagctt
     4081 caaacagaat ggcgaagtga taccagaacg ccacatccat cataccaccc agcgcaccgt
     4141 gcaggatgta agccatcccc accgccagcg tcggcgcacc gcccgcacgg gaaatgatcg
     4201 actgttcacc cacttcgctg gcaatctggt tgagcgtatc cggagtaatc gcaaagcccc
     4261 agctactcac cacctgcgcg gcagaagcga ccacatccgc cgtccctgcc ggagccagca
     4321 ccgccatcgg gctgttcatg gcgaagtaca cgcccggatc gatgatacag gcggaaacca
     4381 gtgccataat cgccacgaag gattccatta acattccacc gtagccgata aagcacgcct
     4441 gcccttcgtt cgccagcatc ttcggcgtgg tgccggaaga gatcagcgca tggaagcctg
     4501 acaccgcgcc acaggcgatg gtgataaaca ggaacgggaa cagattaccg gtccataccg
     4561 ggccagtgcc atcgacaaat ttggtcagcg caggcatggt cagcgtcgga cgcataatca
     4621 aaatgcctac cgccagacca acgatagtcc cgattttcag gaaggtagag aggtagtcac
     4681 gcggggccag cagtaaccac accggcagca ccgccgccac aaaaccgtag cccaccagca
     4741 tccaggtcag ctgcacgccg gtaaagtcaa agtacggtgc ccaggtcggg ctttctgcca
     4801 cccagccgcc agagataatg gcaaaaataa ggaacaccag gccaatcact gacacttcgc
     4861 caatacgccc cggacgcaga tagcgcaggt agattcccat aaaaatcgcc agcggaatgg
     4921 tgaacgcaac ggtatacgtt ccccacgggc tatgagtcag ggctttcacc acgatcatcg
     4981 ccagtactgc aaggataatg accatgatca taaagcaggc taccagcgca atcaccccgg
     5041 cagttggccc catctcttct ttgaccagct cacccagcga gcgaccgtca cggcgcgtag
     5101 aaacaaacag caccatgaaa tcctgcaccg caccggcgag aaccaccccg gcgagcagcc
     5161 agatcatccc cggcaggtag cccatttgcg ctgccagcac cggccccacc agcggccctg
     5221 ctccggcaat ggccgcaaaa tggtgaccga acagcacttt cttgtccgtc ggcacatagt
     5281 ccagcccgtc gttatggcgc accgctggcg tcatacgcgt cgggtcaacc gccagcacat
     5341 ttttggcgat atacagccca taaaaacggt aagcgatcag atagatacag accgacgcca
     5401 ccacaatcca cagcgcgttg atctgttccc cacgatttaa agcaatgtat cccagagcaa
     5461 atgctcccat tacagagagc actgtccaga cgaggtattt ccctgatttg ttcatagttg
     5521 ttatccgtgt gcgtgtccat agagatgtta cattttgttt ctataacatc tttgtacgat
     5581 ttaacaacac ggtaaccatg taaatcggag ttgaaaccaa tatttaactt aacaatgtta
     5641 actcgatcac tccgttaacc gagttttcct gcaatctcat ttaattctgt cgggctcatc
     5701 ccaaaactgc cgtacccagc cgacaagtgc agcaacgccg cccctgttca tcgaaaacga
     5761 tgatttccca gctctgattt tgccgcccaa gatgcagcgg ctggcagacg ccgcgtacct
     5821 tgccttcaga catcgggcga tgatgggtgg cgttaagttc cgtacccacc acacactgcc
     5881 cgtcacgggt catcataaat ccggccatcg atcccagcgt ttcagccagc gccgccgacg
     5941 cgccgccatg cagcaggcca aacggctgat gagtacgggt atcaaccggc atttcggctt
     6001 ccagcacatc atctcccaga cgggtataca caattcccag atgcgccacc attgtgttat
     6061 cgctggtggc gttcagttcg tcgagcgtta aatggcgttt ccagatcatg cttatgcccc
     6121 cagcgttgag ccgccatcga ccacaatatc ctgtagggtg atatggctgg cgaggtcaga
     6181 ggcgaggaac aaaatcgtgt tggcgatctc ttgtggacgg gcgattttcc ccagcggaat
     6241 gccgagttta aactgctcgc caaagccgcg aatacgctgt tcttcggcgt catcgctcac
     6301 ccacagcgtg cgttgcatat cggtgtcggt ggagccaggc gaaaccacat tacagcgcac
     6361 gccgctaacc gccagttcca gcccgacgct caacgccagg cttttcagcg ccgctttcga
     6421 tgcgccataa gcactcatac caatacgcgg cgtgtgtgcg gcgtcggacg ccacagtgac
     6481 aatcgccccg ccccgctgac ggcgaaactg gttcatggtt tgctgaaaca ggttaaacgc
     6541 accgccgacg ttaaccgcaa aagtctgctg ccagtcctct ttactgagct gatcggtcgc
     6601 gcccatgcgc aaaattcccg ccgcattgac cagcacgtcc agtcgctccg tttcagctaa
     6661 cagtcgctga catacctgcg cgacctgcgc agcatcggca acatccatca cttcggtcgc
     6721 aaagggatat tgctcctgag cgaacgcttg atcaaaaccg gtaactttcg ctcccgcttc
     6781 aacaaacgcc agcgccgtgg cgtagccgat acctttacct gcgccggtta cccagacatt
     6841 tttaccgctg aaatccatta tttcacctcg cgggagagta gcttccacca ggcgtcgatg
     6901 gtcgggtttt tcgccagcat gacaaagtcg atatcgccat gcactttgcg ccagcgcgcc
     6961 gccagcgcca tcatgcgcac cgaatccaga ccgtagtcga tcaggttgtc gtcatcgaac
     7021 ggttcatcgg actcgtccag caacggcagg ataacctcac gcagcgccgc tttcctggcg
     7081 gggacaggtg ctggcagtaa tccttcagtc atcaccaccc ggccagaccg cccggccaca
     7141 tatttcagcg acatcaaatg ctcgtcacgg ctgaaatcgg ccagcgcatc cgccaccata
     7201 aacggtttaa tatcgcgcat aaatgcgtcg gttgcggtgg tcatacagcc aatgtgggca
     7261 tataccccgg taataatcag ctggttacgt ccactctctt tgagcatttg ctccagcgga
     7321 gaacgatgaa acgcgctgta gcgccacttc accagcaccg tgtcgtcggc atctggcgtc
     7381 aggcgatcca ccaccttttg ctgttccggc gaacgggtca ggcccggccc ccacatatca
     7441 ttcaacagcg cccgatcttc atcgctctgc tctttcggct gggcggtgta ataaaccggg
     7501 atattgtgct gtttgcagta gtcgcgcagc gcagcaatat tcgcgatcac ctgctccatc
     7561 atcgggcagt tctcgcccca gaagctgaca aaatagtcct gcatatcatg gattaacaac
     7621 gcggcacgtt gcggttcaaa ggcccagtca actttattct gcggaatatc gtgagactcc
     7681 ggcagtgcgt aagcctgtaa ttttggaata gccatcgtgt tctctccttc aggctgatgc
     7741 gcgtgacgcc agccactgac gtaattgttt tttatcgact ttcccgaccg ccgtcagcgg
     7801 aagtgaatcc acacactcca cgcgatccgg taatttaaat tcggcaatac cctgttcacg
     7861 caggaaacga cgcacctgca ccgcgcgcag cggctctttt accaccagat aagcacagct
     7921 tttttcgccc atcagctcat cttccatgct caccagtgcg gcatagatca ccgccggatg
     7981 gcgcagcagc aggttttcga tctcttcggc agcgatcttc tcgccgccac ggttgatctg
     8041 atctttctcg cgcccctgca cggtgatgta accctctgga tcaatagaga tcagatcgcc
     8101 ggaacagtaa aaaccgttgg catcaaaggc actggcattg tgctgcggac ttttgtaata
     8161 accgcggaag gtgtacggcc cgcgcgtcat caggcgtcca acttcccctt gcggcagtgg
     8221 atttccttca gcatcagcaa cccatacttc atcgtccgga cacattgggt aaccctgggt
     8281 atggataatt ttctccgcgc tatcatcaag tcgggtgtag ttcaccagcc cttccgccat
     8341 gccaaacacc tgctgcaact ggcagccaat ctcagcggaa atacgcgccg caagggtggc
     8401 agaaagacgt gcgccgccga cctgtaacag tttcagcgag gcaagctgcg cccggctttc
     8461 gccttcagcc agcgcctgca accacaggct aactgccggc ggcaccagcg cggtaacgtt
     8521 aacctgatgt tttgcaatca acgggaagca aagcgtggcg ctgggatcgg ccgccagaac
     8581 aaccgttcct ccggcaagaa agacgcccag cgatcctggc gaactcatag cgtagttatg
     8641 agccgccggg atcgcgcaca ggtagcgtgt ctgttgtgtg aactgacaaa tctcgacgct
     8701 acgacgcacg ctgtagtagt agtcgttatg agtgcgcggg atcagcttcg gtgtaccggt
     8761 ggtgccgccg gaaagctgga aataggccac ttcatcagca ggtgatggcg tagcagtaaa
     8821 accgtctgcc ggatggttaa tcgcatcctg caagttatgc tcaccgctgt cgttgagcag
     8881 ttgcaccacg cgaatggagg aatgttctgt gacgaaagta ttgaggaaat catccccgct
     8941 aaacagcgca tgttggcgat cggcaatcag taacgccggt tcaatctggc tggcataggc
     9001 gttcagttca ctacgctgat ggctgaacaa cgccagcacc ggcgcaacgc ccagtttcag
     9061 cagtgcgaaa aaggtaatgt aaagttcagc gacgttaccc agttgtacca gcgcggtttc
     9121 accgggttta atgccctgac ggcgtaaact acacgcgagg ttatccgccg cctgattcag
     9181 ctcccggtaa ctcaactgtc gctcgccgtc gataaccgcg atgctgtcac tcgcagcgtg
     9241 gcgagtcaga atgtcggtca gcggcaaatc ctgccagtag cctttttccc gatagcgacg
     9301 ggcaaactct tccggccagc gggtgaatgg aatgctcatc ctcgctcctt aatgcaatcc
     9361 aaaaacgttc aacatggtag aaagtttgac gcctgtttcg cgccactcac ccaacggtga
     9421 cgacgcaggc acaatccccg ctccggcaaa cagacgcacc tgattttccc gcagcttcgc
     9481 gcagcggatg gtcaccaccc attcgccgtt accttcgctg tcacaccaac ccacaatgcc
     9541 gccaaacagt tcgcggtcaa acggctccag ttcagcaata acctgggtcg cggcctgatg
     9601 cgggaaaccg ctcagcgcgg gggtcggatg cagcagacag gccagagtca gtgcgttttc
     9661 ttgcgaattc gctttacctt caaagggagt tgcgagatgc cacagcgtcg gcgtggtaat
     9721 caattgtgga gaggagggaa cgtgtaactc actactgcgt tcgcgcagta cctctttcat
     9781 cgcctgagtc accagttcat gttcatggcg atctttttct gacgccagca gacgattacc
     9841 cgcttcgcga tcgagcactt catccggctg acgacgcgcg gaaccggcta acggaatgga
     9901 gctaaaacgc tcgccgtctt tacgtagcag cagttccggg ctggccccca gcaggacgcc
     9961 accatcagcc agcgggacat ggaagttgta actaaccggg ttttgcgcaa tcaaccgttc
    10021 cagcaatacg ccactatcaa tggcggcgtc agtggtgata tcaatcaatc gtgacaacac
    10081 cactttgtcg acctgcggcg tggcggtaag tgcggcggcg cgggcaacca tctgttcaaa
    10141 cgtggtttgt tcaggaattg cctggcgttc caccacgttc agcgactggc tgcgggtaaa
    10201 acagtgggct gaggtctgtt tttcctgacg ggagaacgac tgccaggatt cgggaatata
    10261 cagcgacgaa ggctgacgtg gatcgaaggg aatcgccccg accatcaccg gatttttgat
    10321 gccctgcgct ttggcatcgg caaacagcgc ggcgagtttt tgctggaagg gactgtcggg
    10381 cgaatccccg ttcacagccg gttcatcgaa gcgggcgaaa catcctgacg tcgtaaaact
    10441 gcggtacggc gacataaaga aaaagcgatt gggcgcaagt gttgccatgg tctgctgtac
    10501 ttcctcagcc agtgacgtat ccatatcatc ctccacaaaa tgataaaggc tttaataatg
    10561 attatcattt atattttcgg tcgctaacct aaaagcaaac gcgcactatg tcaactcttg
    10621 aggtaacgca atctgcaact cggggttgca tctggttgtg gttacaatga aaatgagaag
    10681 cattattgat gaattcgcat aagcgcaatg tgatgtcctg cgccgttttg ccccctctcc
    10741 cttccagggt gagggctggg gtgagggtta atgttcgcac cagtgctggc tgttcccctc
    10801 accctaaccc tctccccaaa ggggcgaggg gacggattgt gcgctttgtc gaatttgtca
    10861 ttacgccctt aaccttatta ataacaggaa gctgacttgt gagactcgcc ccgctctacc
    10921 gcaacgccct tctattaaca ggacttttgc tttcaggaat agccgtagtt caggccgccg
    10981 actggccgcg tcagattact gacagccggg gcactcatac cctggaaagc cagccgcagc
    11041 gtattgtttc caccagcgtc accctgaccg gatcactgct ggcgattgat gctccggtga
    11101 tcgccagcgg cgcgaccacg ccgaataacc gcgtcgcaga tgaccagggc tttttacgcc
    11161 agtggagcaa ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc gaaccgagcg
    11221 ccgaagccgt tgccgcgcaa atgccggatc tgattttaat tagcgcaacc ggcggggatt
    11281 cggcgctggc gctgtatgat cagctttcca ccatcgcccc gacattaatc atcaattacg
    11341 acgacaaaag ctggcagtcg ctgttaacgc agcttggcga aattaccggg catgagaaac
    11401 aagcggcaga gcggattgca cagtttgata agcaactggc agcggcgaaa gagcaaatca
    11461 aattaccgcc gcagccggtc actgccattg tctataccgc cgctgcacac agtgccaatc
    11521 tctggacgcc ggaatcagca caagggcaga tgctggaaca actcggcttt acgctggcga
    11581 agttgcccgc agggttaaac gccagccaaa gccagggcaa acgccatgac atcattcagc
    11641 ttggtgggga aaatctggct gctgggttaa acggtgaatc gctattcctg ttcgccggtg
    11701 atcagaaaga cgccgatgcg atttatgcca atccactgct cgcgcacctg cctgcagtac
    11761 aaaacaagca ggtttatacg ctgggaaccg agacgttccg cctggattac tacagcgcca
    11821 tgcaagtgct ggacagactt aattcactgt taaaaaaaga ttgataaaac atacaattag
    11881 agcactaata atcccctcgc ccctttgggg agagggttag ggtgagggga aaatgcacct
    11941 ttagctaact gtcagacgct gtcacctgcg gcggcgtctg gcgaaaacgt cgcaactcca
    12001 ccagcaccag caataacaac acgccgataa tcaacaaacc aaaaccgctc gcgcttgcgg
    12061 aagcaaccgg tgtcatcatc gcgcccaaac cacccaacag cgccgcgcct atggcatcgc
    12121 ccgtcacgtt ctgcgccgtc cacaaaccgt taatccgccc taacatcgct tccggggttt
    12181 gcgtttgcag cattgtatat tgcagcaacg aactaaccgc gctcagccag ccgaacagcg
    12241 ccagacaaac cacgcctaaa atccacatcg gcatcaggcc aaacagacca atggcgagga
    12301 acgatcccag cgtggagagc agcatcaata accctggtcg cgcactatgt gccagcttcc
    12361 cgctggttaa cgcaccaata gccgcaccga gcgggatcgc cgcatagaga aaaccaatct
    12421 gtgccgccga catctgccag ttgtcagcca gcgccggata cagtacccgc accgcgctcg
    12481 ccatcgtcaa taaaccaccc agcaacgcaa tccctcctac cagcgggctg gcgagcagaa
    12541 aacgaaatcc tgccagtaat gatttcaacg gatgctcgcg cggctgcggt ggcggtggca
    12601 acgccggaag gcttaacaac ggtagcaagg taataaacgt gcccgccgcc gccagcccgt
    12661 agttccaggc tacgccgccg gtcgccagca ataaaccgcc aatcatgggc gaaatcaccg
    12721 accccagacg cacggtcaac atggtgatcg ccccggcctg cattaagttt tcacgcccta
    12781 ccagtgcagg tgttgccgcc agtagcgccg taacaccaag tgatgcgaaa aaaccatccc
    12841 ataaaccaag taaatagatt gccagcaatg acggctccgg cagcagcgcg ttcaggcaca
    12901 gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa aatcactttt ttgcgctcat
    12961 agcgatccgc cagcacaccg ccaaccatca ggccaacaaa catcgcgccg ccggtcagcg
    13021 tcaccgaaag ccccacctgc caggtagaat gcgtcatcat ctggatctgc accggcaccg
    13081 cgacgccgag caaacccaga gacacaattg agataaaacg agcgaggaat actgcgcgaa
    13141 acgccgggtg cgttttcaac aggctgaggt taagcagcca ggattgttta ttcattacaa
    13201 tgccttgcca tcaaatattt taaataccta ttcttaggct gcacatgcta acatatccaa
    13261 ataagatcga taacgataat taatttcatt atcatggaag ttcgtatgtc tggttctgtt
    13321 gccgtgacac gcgccattgc cgtgcccgga ttgctgttat tactgattat cgcgacggca
    13381 ttaagcctgc tcattggggc aaaatcactc cccgcgtcag tagtgctgga ggccttctca
    13441 ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg cgcgactgcc gcgcaccctt
    13501 gccggtttac ttgcaggcgg tgcgcttggg cttgctgggg cgttaatgca aaccctcacc
    13561 cgaaatccac ttgccgaccc cggcttgctt ggcgtgaacg ccggagccag ctttgccatt
    13621 gtgctgggtg cggcgctgtt tggttactca tccgcgcagg aacaactggc gatggccttc
    13681 gccggggcgc tggtggcctc attgattgtt gcctttatcg gcagtcaggg cggcgggcag
    13741 ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg cggcggtgct ggaagggctg
    13801 accagcggca tcgccctgct taatcctgac gtctacgatc aactgcgttt ctggcaagcc
    13861 ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg tgctgatccc ggttctgatc
    13921 gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca gtttgagcct cggcagtgac
    13981 accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt tgattggtct gctggcgatt
    14041 accgtgcttt gtggtagtgc gacggcggta gttggcccga ttgcctttat cggcctgatg
    14101 atgccgcaca tggcgcgctg gctggtaggt gccgatcatc gctggtcgct gcccgtcacg
    14161 ctacttgcta cccctgccct gctgctgttt gccgatatca tcgggcgggt gattgtgccc
    14221 ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg caccggtgct gatcttcctc
    14281 gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc tcgccgatta ctcatcacct
    14341 gtttgctgct gatttccgcc tgtgtggttg caggtatctg gggattacgc agcggtgccg
    14401 tcacgctgga aatctcgcag gtattcgccg cgttgatggg cgatgcgccg cgcagtatga
    14461 cgatggtggt caccgaatgg cgtttaccac gcgtgctgat ggcgctgttg attggcgcgg
    14521 cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg taacccgctc ggcagccctg
    14581 acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct ggtggcgatg gtgctgtttg
    14641 gtcaggacct gacggctatc gcgctggcag caatggtggg cggcattgtc acttctctgt
    14701 tggtctggct gctcgcctgg cgtaacggta tcgacacctt tcggttgatt attatcggca
    14761 tcggcgttcg cgccatgctg gtggccttta atacttggct gttgctgaaa gcgtctttag
    14821 aaacggcgct aacagcgggt ttgtggaatg ccggatcgct caatggcctg acgtgggcaa
    14881 aaacttcgcc ttccgcaccc atcatcatat tgatgctcat tgccgccgcc ttgctggtgc
    14941 ggcgaatgcg cttgctggag atgggcgatg ataccgcctg cgcgctgggc gtcagcgtcg
    15001 agcgttcgcg tctgttaatg atgctggttg cagtggtgct taccgctgcg gcaacggcgc
    15061 ttgccgggcc gatttccttt attgctttag tcgcgccgca cattgcccga cgcattagcg
    15121 gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg agcgctgtta ctgctggcgg
    15181 ccgatctctg cgcccaacaa ctgtttatgc cgtatcaact tccggttggc gtcgttaccg
    15241 tcagcctcgg cggtatttac cttatcgtct tgttaattca ggagtctcgc aaaaaatgac
    15301 cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg ggatatggca aatataccgt
    15361 tgcggaaaat ctgactgtag aaattcctga tggtcacttc acggcaatta tcggaccaaa
    15421 tggctgtggt aaatccacgt tactgcgtac cttaagccgc ctgatgacgc ctgctcacgg
    15481 gcatgtctgg ctggatggcg agcacattca acattacgcc agtaaagaag ttgcacgccg
    15541 gattggtctg ttggcgcaaa atgctaccac gccgggcgat atcaccgtgc aggagctggt
    15601 ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc tggcgcaaag aggatgaaga
    15661 agcggtaacg aaagcgatgc aggccacgag aataactcat ctggcagatc aaagcgtgga
    15721 taccctttct ggcggacaac gccagcgagc gtggatcgcg atggtgctag cccaggaaac
    15781 ggcaattatg ctgctcgacg aacccacgac ctggctggat atcagtcatc agattgatct
    15841 actggaactg ttaagcgaac tgaaccgcga gaaaggctat accctggcgg cggtgctgca
    15901 cgatcttaat caggcctgtc gttacgccag ccatttgatt gcattgcggg aagggaaaat
    15961 tgttgctcag ggagcgccga aggagattgt cactgctgaa ctgattgagc gcatttatgg
    16021 tctgcgatgc atgatcattg acgatccggt agccggaacg ccgcttgtgg tgccgctcgg
    16081 acgaacggca ccttcaaccg caaatagtta aactaagtgg tctgccatca tggcatcctg
    16141 ttttctggat gccatcgcat agcgcaataa cacgctacca caagccacca tcccgccgat
    16201 cagcgcggaa aggatcacaa taatcgcctt acccggaccg tcttttttca ctggcaatga
    16261 cgggcttaac tgatatttaa acggcgtaaa attcacatcg ttgacatttg cttttgttaa
    16321 ttgctcgaca agatactgcc gattacgtaa ttcaccgttc agttccgcaa cgtcagtaac
    16381 cgctttttca atttccagtt tgcgttcaat accgtctgcg ccgagagaaa tagagaaatc
    16441 ggggtcatct ttaacggcct gaccattact gtatacgggc tttttaattc ctgccgcgtt
    16501 ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca tcaagttgat ttttcgtttt
    16561 aatgcgatcc tgagccagtt tttctttttc aaactgggtt ttgatctcca gtttattacg
    16621 gacgttttct atcgactctt tcaccaccaa cgcagagata taatcgatat acccgctcaa
    16681 aacggtctgc gcctctttac tggttggcgc ggtaaaactt agcgtccagg aggtatacag
    16741 tgacggttca tcttttttct tactggcatt gtcatcaacc gctttcattt tttcgctcaa
    16801 tgcaacaata gcgcgatgca aatccagttc atcgattttc gcctctttta attggtccat
    16861 cacataaggt gatgaacgca ggtactcttc cagcaagcta acagactgaa acttcttgat
    16921 aaacaggtta aatgcaccgc cacggtcgat attaacatcg agatcaagaa cgcggagttt
    16981 ggtaaaggtt ttctccaggt cctgccactg aatggcttcg gcgggagtaa taacggctga
    17041 gctggtccat ttttgcggca ggatgaaaga gatcagtaaa cctgcgcagg caaatgcaaa
    17101 aacgaccgcc atgaccgttt ttttggcccg ccataaaact tcgattagat taagtaaatc
    17161 aatttcatta ttactgggcg acgccagagg atactcagaa aaatgagcgt cacttccctg
    17221 tttaatattc aatgatgaca tggggtataa cctgaaaatg agtccaatag gggaaaatct
    17281 ccacggcgct cactttacca gttggtgaga attatccgaa gctgaagttt gtaaattcct
    17341 tccactgaat gcaatattca ctctatttaa gagaaatatt catcaaacct ataaatcaaa
    17401 caaataaaaa acccataatt attttctgta attatgggtt tatagataat attaatttac
    17461 ctgtttagcg ttgcgcgaat aatcggccca atttttacaa acgcccctgg agagataata
    17521 tccacatgcg cacaatcctg acgatagata tccagctccg caatccacgg cgaccaggcg
    17581 cgttcaggac tcataccttc ctgaagcgtg cgttcagcaa caaacaacgt cgctttaccg
    17641 tcaaacggta cgctatgagc agtcgtcagc aggcgcacag catcagcgta gttgccttca
    17701 atggtggtaa acaactccgt tgaagtactt ccctgctgtg ccgccaggaa ggcctcgcgc
    17761 tcgcggttaa tctccgccag cacttccggg tccagaccat tagcttcttt ttcctgccag
    17821 ttttgcgttt ctggcggcca ggtatccagc aagccaagaa atgccacctg ttcgccacgg
    17881 gcacgcagtc gcgccgcaat accctgcgcc agcgtaccgc caagggaata ccccagcagg
    17941 taataagggc cgtgcggttg ttgctcaagt aacgttgcca gatgcgcttc gcagacttca
    18001 tccaggtttg tcgctgtctg catgggtcca tgagggcgcg gcgactgaat gccaataatc
    18061 gaccattgtg gatcgagata acgcgagagt acgctaaact gccaggcaaa accggacgcc
    18121 ggatggaaac aaaacagcgt cgggccatta ccttcacgca acggcagaat ggtttcgaat
    18181 cccatgcgcc gggagctgtc ctcttcacca tcaataatcg ttgccagttt ggcgacggtt
    18241 gacgcgacca tcacctgccc cggcgtcacc tgacgagcaa actgtcgact taactgcgct
    18301 gccagtttca ttgccagtag cgaatgaccg cccagcgcaa agaaatcagc atcggcatcc
    18361 tgcacgtcac aacccagcaa cgacgcgaat gccgcggcga taatcgtttc actgcccgct
    18421 ttcggtgcac gtcccgacgc ttgtgtcttc agttcaggca acggtaaggc tttgcgatcc
    18481 agcttgccgt tggcgctaag aggtaactgt ggaagttgca gcagaaccac cggtaccata
    18541 tgtggtggca atgtttcacg aagctgcgcc tgcaatgcgc tggtatccaa cggcaggccc
    18601 gattgcgaca ccagatagcc caccaactga cgcgcatcac caccagtggc tgccgcctgg
    18661 ttaatcacac aggcgtgggt aacggcttgt tcgacatccg gcagcgcctg catcacgcga
    18721 tcgatttcgc ccagttcgat acgctgcccg cgaattttta gctgatcgtc actgcgcccg
    18781 aggtactcca ccgcgccgtt atccagccag cgggcaacgt ctccggtacg gtacatccgt
    18841 tcgccaggag caaaaggatc ggcaataaag cggctggcgg tcagatcggg gcgtccgaga
    18901 tagccctgcg ccagttgaat gccggtaaga tagagatcac ccgccacacc cggcggcacc
    18961 ggatgcatca tcgcatcaag aatacgcagc cccatgttcc acaccggata accaatcggc
    19021 acactgctgc cgcgcacctg tgccagttcc tcgccaaaag ccggatacca gctgacatct
    19081 accgccgctt ccgtcgggcc atacagatta tgtaacggcg caccggttaa ctgttgccat
    19141 tcacggcata aatcagccgg taaggcttca ccactacaga aaacctgttt caacgtcgcg
    19201 caactctggc gagcggtttg cggagtcagc gaggcaacaa atgccgccag catcgacggc
    19261 acaaagtgcg tggtcgttac gccatattcg gcaaagaatt gctgcatagc gagcgggtca
    19321 cggtgcgctt ccggttcagc catcaccagt tttgcccctg cgataaacgg ccagaaaaac
    19381 tcccacaccg agacatcaaa actgcacggc gttttttggg caacgacatc ttcgcctgta
    19441 agcgggtagt gattttgcat ccataacagg cggttaacga tagccgtctg cccgaccatt
    19501 acccctttcg gcctgccggt ggagccagag gtaaagatga tataagccgt gtggtgcggt
    19561 tgtgaaagtt gcagcggcgc actgccctgg ggggtaagcg gggcgttata gcaaaggctt
    19621 gttaaattgg gaacatcgct aaagcgcggc agttgatcgt cggtggtaat taacagcgac
    19681 ggacgcgcat cttccagcat cattttcagg cgatcgtccg gatagccggt atccagcggt
    19741 agccaggccg cgccagcttc aactattgca tggagtgcca gggtcaaaaa gaccgagcgc
    19801 ggtagtgcca ccgccacgct gtcccctggt ttaacgccgc gctcacgcag cagattcgcc
    19861 agcgccacca cctgctcgcg catttcccga tagctgaaca ggtaacgcgc atctgccagc
    19921 gccggagcat ccggtgtttt tgccgcttgt tctgccacca gcgcgctcag cgtggtttct
    19981 ggaatctcaa cctgagtggc gttgatctgc gccagctgcg catactcacc tggcagcata
    20041 atatcgacat cgccgcacaa cagcgccgga tccgcggcaa actgggcaat cagcattttc
    20101 aggcgttcag catgctggat taacgttggc tcatcgtaac gctgtttatt ggcgaggatc
    20161 tcaatactca aatcaccgtg ttcatccggg aacagggcca gttcaaggtc attaaccgga
    20221 ccggttgcca gggtatgggt ttgcgcctga acaccaggaa tatccagttg gtaatcaaat
    20281 accttaatat tgagtaccgg accaaacagc ggttcatcac ctgccgctcg cccgctgtca
    20341 cggacaattt gttcggcatc gtaacgttga tggcgacgca tttttttcag ttgagccgcc
    20401 agtcgggttg ccagttccgg cagcgtttct tgcgccgcaa tgtgaatacc caacggtaaa
    20461 acgttgagca cgggtccgct agccgtcagc gccgccgagc ccagtcgacg cataaagata
    20521 aatccggcgg catagtccat gcgattgcac aatcgcccca gccacaaggc tgccagcgca
    20581 agggctaaat cggtacgctg cacacctgaa agttgcgtag ccagctggcg gaattccccg
    20641 tcggtaaatt ccagtttcag gcgcagaata tctgccgaag cgctgcgccc cggtaaaggt
    20701 gccggagaaa gtgacgcggg cggcggcagt tgacgacgct gttctgccca gaatgccgca
    20761 tcacgctgcc aggcttcgct ttcgcggtat tgctggtact cttccactac atcagcgaaa
    20821 ggcgtaaacg gcgaagcagg cgttggttcg ccacgcagcc atgtgcagta aatattggcg
    20881 atctggcgag taatggccgg gaaactgaag ccatcgacca gcaaatggtg ataacgctga
    20941 taccagtacc agcggttatc cgcaacatgt atcagctgat gaaagaccag tggtttaccg
    21001 ctatcgacgc gcaaatcttg ttgcaaatcc gccagcatta atgcctgcgc agtaccgtgc
    21061 ggatcaatat tggttcgcag gtcgataatt tctggctgtt cgaatatcag cgcatcatcg
    21121 acccactgcc agacttcgcc gttatcttcc gtaaaacgca tccgcaacgt atctgcttgc
    21181 gctagtccgg caaccaccgc gcgggccagt aatggcgcat caacatctcc agttaactca
    21241 acgtaatgcg ccacgctcca ggcggagggt aaatccgaca gtttttctgc catccagatg
    21301 ccgggctgtg cggcgaccaa aggtaaatgc tggctcattg tgcctcctgc aactgggtga
    21361 aattggccgg tgtcagagta cgccagtgga cttccagcca ctgctggcag gacgcctgtg
    21421 actgcggctg acacacaatg tcccagcctg ccgataagag gcattgttgc ggccacagac
    21481 tgaattgccc ctgcgcattg cgcaaaatgt aaaacgctcc ctgcggatca tcgaagggat
    21541 tactgaatgc catactcaac tcctgtcatg gaaaagtggt tgccagaggt cgattaaccc
    21601 ctgcatcaag ccaccgcgcc aacaaagcgc atcatgtccg ccgtcaacct gacgccagaa
    21661 aatggattct tttatagggt gcaattgcgc atacagcgcc tgattggccc gcatgatcat
    21721 cggctcgcga atacccgctt ccagcacaat gcgcagacct tcggcgctaa cttcaccagc
    21781 ttttagcttt tcaagtaaca cgccctcttg ctgcccgtcc cgatgcggcc accagtacga
    21841 tcctgactgg cttaatacac agccaaagcg ttcaggccag tgcagtccgg catacagcgc
    21901 ggacagccca ccaaaactct gcccggcgac cacggtgcga tcggcgcgat cgctaaaagg
    21961 ggcaatagct ttcaccaggg gtaataactc ttgctgtact gccagccaga aatccgcatt
    22021 acacggcagt tcgtgggcgc ggtgcgtggt gtcgatagcg tcgatcaaca catacacggc
    22081 gggaggaagt tgctgacgat gggtcagcga agtcagcaca ggccagacgg gcatactttg
    22141 cgcccaaaat tcgccatcga gcaaaactgc cagcggacgt tcttctgctg ttacatcgcc
    22201 ggtggtaaaa atccatacac gacgtgaatt tttcaaccgt tcacttttcc agataatttc
    22261 tttggctgct atttctggca cttgcggaca atcccatccc ggttgcagag gcgcttgcgg
    22321 catttcgagt gcagaaacag cgtgccctcg cccgcctttc cagctttgtg gattcagcgg
    22381 atcggctatt gcctggggta atagttttcg ccagccttca cgtaattcga ggcgatcggg
    22441 ggatggtgca gaaaaaatgt catcgcgttc ggtgggaata aagcagtagc tgccgcgcca
    22501 gttggcattg agttgtgtcg tccactgcca gacatcagta cctgcaattc gctgcatcga
    22561 ctggggctgg ctgttctgat gatgatcggt cacaccagtg atgtagatcc atacgcgctt
    22621 tatcgtcgag tattcttcag aaccttgggg atcacgccac cagaaagtga cctcaaacat
    22681 ttcgtcattc agacgctgcc attccgggcc atgtttcgac tgccaccagc tctcacttcc
    22741 tacttttaac gccgtcacac cataacccca tgtttactgt gcaatttttc attgattgca
    22801 gaaatatatt gataatatta ttgataacca tttgcatttg caatagcgta atggcgcgcc
    22861 gtgggaagcg cggacattta ttaaccaact gcactgcgtg tctttcagga tcaaaggttt
    22921 tcgcggtagc gggatgcgtc gtgttgagga cgaccatgcc cgacagttgc aattcgtggc
    22981 aaaaatgcag gaataaaaca atgaacaaga agattcattc cctggccttg ttggtcaatc
    23041 tggggattta tggggtagcg caggcacaag agccgaccga tactcctgtt tcacatgacg
    23101 ataccattgt cgttaccgcc gccgagcaga acttacaggc gcctggcgtt tcgaccatca
    23161 ccgcagatga aatccgcaaa aaccccgttg cccgcgatgt gtcggagatc atccgtacca
    23221 tgccaggcgt taacctgacc ggtaactcca ccagtggtca gcgtgggaat aaccgacaga
    23281 ttgatattcg cggtatgggt ccggaaaaca cgctgatttt gattgacggc aagccggtaa
    23341 gcagccgtaa ctcggtgcgt cagggctggc gtggcgagcg cgatacccgt ggtgatacct
    23401 cctgggtgcc acctgaaatg attgaacgta ttgaagttct gcgtggtccg gcagctgcgc
    23461 gttatggcaa cggcgcggcg ggcggcgtgg ttaacatcat taccaaaaaa ggcagcggtg
    23521 agtggcacgg ctcctgggac gcttatttca atgcaccaga acataaagag gaaggtgcca
    23581 ctaaacgcac caacttcagc ctgaccggtc cgctgggcga cgaattcagc ttccgcttgt
    23641 atggcaacct cgacaaaacc caggctgacg cgtgggatat caaccagggc catcagtccg
    23701 cgcgtgccgg aacgtatgcc acgacgttac cagccgggcg cgaaggggtg atcaacaaag
    23761 atattaatgg cgtggtgcgc tgggatttcg cgccattgca atcgctggaa ctggaagcag
    23821 gttacagccg ccagggcaac ctgtatgcgg gcgacaccca gaacaccaac tccgattcct
    23881 atacccgctc gaaatatggc gatgaaacca accgtctgta tcgccagaac tactcgctga
    23941 cctggaacgg cggctgggat aacggagtga ccaccagcaa ctgggtgcag tatgaacaca
    24001 cccgtaactc gcgtattccg gaaggtctgg caggcggtac cgaagggaaa tttaacgaaa
    24061 aagcgacaca ggatttcgtc gatatcgatc ttgatgacgt gatgctgcac agcgaagtta
    24121 acctgccgat tgatttcctc gttaaccaaa cgctgacgct gggtacggag tggaatcagc
    24181 aacggatgaa ggacttgagt tccaataccc aggcgctgac cggggcgaac accggcggcg
    24241 ctattgatgg tgtgagtgcc accgaccgta gcccgtattc aaaagcagaa attttctcgc
    24301 tgtttgccga aaacaacatg gagctgactg acagcaccat cgtaacgccg gggctgcgtt
    24361 tcgatcatca cagtattgtc ggcaataact ggagcccggc gctgaacata tcgcaaggtt
    24421 taggcgatga cttcacgctc aaaatgggca ttgcccgtgc ttataaagca ccgagcctgt
    24481 accagactaa cccgaactac attctctaca gtaaaggtca gggctgctat gccagcgcgg
    24541 gcggctgcta tcagcaaggt aacgatgacc tgaaagcaga aaccagcatc aacaaagaga
    24601 ttggtctgga gttcaaacgc gacggctggc tggcgggcgt cacctggttc cgtaacgatt
    24661 atcgcaataa gattgaagcg ggctatgtgg ctgtagggca aaacgcagtc ggcaccgatc
    24721 tctatcagtg ggataacgtg ccgaaagcag tggttgaagg tctggaagga tcgttaaacg
    24781 taccagttag cgaaatggtg atgtggacca ataacatcac ttatatgctg aagagtgaaa
    24841 acaaaaccac gggcgaccgt ttgtcgatca tcccggagta cacgttgaac tcaacgctga
    24901 gctggcaggc gcgggaagat ttgtcgatgc aaacgacctt cacctggtac ggtaagcagc
    24961 agccgaaaaa atacaactac aaaggtcagc cagcggttgg cccggaaacc aaagaaatca
    25021 gtccttacag cattgtgggt ctgagcgcga cctgggatgt gacgaagaat gtcagtctga
    25081 ccggcggcgt ggacaacctg ttcgacaaac gtttgtggcg tgcgggtaat gcccagacca
    25141 cgggcgatct ggcaggggcc aactatatcg cgggtgccgg ggcgtatacc tataacgagc
    25201 cgggacgtac gtggtacatg agcgtaaaca ctcacttctg atgctaacgt cagattgttg
    25261 acaaagtgcg cgtcgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaaaat
    25321 cttgccaatt caatatattg caggaccatg taggcctgat aagcgtagcg catcaggcag
    25381 ttttgcgttt gtcatcagtc tcgaatatgg tcgatatgaa aactacgcat acctccctcc
    25441 cctttgccgg acatacgctg cattttgttg agttcgatcc ggcgaatttt tgtgagcagg
    25501 atttactctg gctgccgcac tacgcacaat tgcaacacgc tggacgtaaa cgtaaaacag
    25561 agcatttagc cggacggatc gctgctgttt atgccttacg ggaatatggc tataaatgtg
    25621 tgcccgcaat cggcgagcta cgccaacctg tctggcctgc ggaggtatac ggcagtatta
    25681 gccactgtgg gactacggca ttagccgtgg tatctcgtca accgattggc attgatatag
    25741 aagaaatttt ttctgtacaa accgcaagag aattgacaga caatattatt acaccagcag
    25801 aacacgagcg actcgcggac tgtggtttag ccttttctct ggcgctgaca ctggcatttt
    25861 ccgccaaaga gagcgcattt aaggcatcgg agagacaaac tgaagcaggt tttctggatt
    25921 atcagataat tagctggaat aaacagcagg tcatcattca tcgtgagaat gagatgtttg
    25981 ctgtgcactg gcagataaaa gaaaaaatag tcataactct gtgccaacac gattaattga
    26041 caacatctgg tacgattcgc ccgcagccat cactgactgc gggcgaaagt gtaaagcagg
    26101 tgccttacca ctctgacctg acaaccggat atgcagggat tgctccccgc acaacggcta
    26161 cttcttcggt tcgtaagcga gaacagcctt aaactcaata ttacgttcct ttacggtgaa
    26221 ctcacacagc gagtctccga ccagaagcgt aaatcccagc accgtcaaac acagcactat
    26281 gactgccaca agggcatatt tcgtcagcat gtttatattg cctccatgcg aagaagggac
    26341 taccatccga gttgttgagg ttcggagtgg cagccccagg attgatagaa atatctcctg
    26401 gggcttcatc tttccagacc tcagagtaaa cctgaaaccg gaaagcttca ggcacccgcc
    26461 gttatcttac ccttcctcag caaaatatct gtttatttat acagtaagtc ctgtttcgga
    26521 cttctgttgg caacactcgt tattgctcgt aaaacgaaat cgcccgcagc catcactgac
    26581 cgcgggcgaa agtgtaaagc aggtgcccta ccatcctgac ctgacaaccg gatatgcggg
    26641 gattactccc cgcacaacgg ctacttcttc ggttcgtaag cgagaacagc cttaaactca
    26701 atattacgtt ccttcaccgt aaactcacac agcgagtctc cgaccagaag cgtaaatccc
    26761 agcaccgtca aacacagcac tatgactgcc gcaagggcat atttcgtcag catgtttata
    26821 ttgcctccat gcgaagaagg gactaccatc caaattgttc gggtttggag tggcagcccc
    26881 agggttgata gaaatatctc ctggggcttc atctttccag acctcagagt aaacctgaaa
    26941 ccggaaagct tcaggcaccc gccgttatct tacccttcct caacaaaatg gctgtttatt
    27001 tatacagtaa atcctgtttc ggacttctca ctcctcattt aatcaactga agctgagccc
    27061 gttttttctg catgcctcaa tcgtcaaaat tcttcgccat cagccagctc tgccataact
    27121 tgccacattg aaaaagtagc caaattgctt cagtcaccat catcaaaaaa agaaacattc
    27181 cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa aacgccaaca
    27241 taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac cctcggtatt
    27301 gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga ctcttcaatg
    27361 ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga tatcaccgaa
    27421 agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc cgggcaattt
    27481 tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga aatttgcggc
    27541 ggtggcacac acccgtttca gaaatggcag cgtcaggagg tatgcgataa cgagcgctat
    27601 caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt tggtcagcat
    27661 gtccatgttg gctgcgccag tggcgatgac gccatttatt tgctgcacgg cttgtcacgg
    27721 tttgtgccgc actttatcgc cctttccgcc gcgtcgccat atatgcaagg aacggatacg
    27781 cgttttgcct cctcacgacc gaatattttt tccgcctttc ccgataatgg cccgatgccg
    27841 tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtttgagtta caccacgatg
    27901 atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt tggcacggtg
    27961 gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat ggcgggatta
    28021 attcaggcta ccgcccactg gttactgaca gaacgcccgt tcaaacatca ggagaaagat
    28081 tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atgggctgga aggcgtcatt
    28141 accgatccgc acactggcga tcgtcgaccg ctaacggaag acaccttgcg attgctggaa
    28201 aaaatcgccc cttccgcaca taaaattggt gcatcgagcg cgattgaggc cctgcatcgc
    28261 caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc cgatggtggc
    28321 tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccgatgacta agtagggaat
    28381 tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac aatgttatga
    28441 aacacccttt agaaaccttg actaccgcag caggcatttt gctgatggct ttcctctctt
    28501 gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg gtgaccatgt
    28561 tccatctgat ggatcttagc tagctttaca ctttattgtt ttgtctgtgg tttttagtgc
    28621 tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc tcgctggcgg
    28681 attaaacgtc gattatcgcc gtacttcgcg gcatactttg cttattctcc ttcgcctaaa
    28741 ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctggc ccttgagtta
    28801 ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa aattggcggc
    28861 aaaattttta tgctgtcgtc ggagctgcgc ggcgtcccct ttatcaatct gaagtccgat
    28921 ccacaaaaat ccctgttaaa tcagcagata tacccgagca ttaagccagg gtatcacatg
    28981 aataaaaagc actggatttc ggtgtatccc ggcgaggaaa tctccgaagc gttacttcgc
    29041 gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg cgatcaaaaa
    29101 agagtgcgtc caggctaaag cggaaatcta tagcgcattt ttctcgctta ccatttctcg
    29161 ttgaaccttg taatctgctg gcacgcaaaa ttactttcac atggagtctt tatggatatc
    29221 atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagtaa aaaacttacc
    29281 ccggaacagg ccgagcagat caaaactctc ctgcaataca gcccatccag caccaactcc
    29341 cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt tgccaaatcc
    29401 gctgccggta attatgtgtt caacgaacgt aaaatacttg atgcctcgca cgtcgtggtg
    29461 ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga ccaggaagat
    29521 gctgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg tcgcaagttc
    29581 ttcgctgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc aaaacaggtt
    29641 tatctcaacg tcggtaactt cctgctcggc gtggcggctc tgggtctgga cgcggtaccc
    29701 atcgaaggtt ttgacgccgc catcctcgat gcagaatttg gtctgaaaga gaaaggctac
    29761 accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa cgctacgctg
    29821 ccgaaatctc gtctgccgca aaacatcacc ttaaccgaag tgtaattctc tcttgccggg
    29881 catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt ttcagccgtc
    29941 atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc caggttgaag
    30001 atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc ggtattattt
    30061 ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg accttcgaaa
    30121 aacgagccat cgccagttca cggctttggt tgcaaatcat aacccagaat aaactcttcc
    30181 accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta ttctggctgc
    30241 aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg atcatgatgt
    30301 atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg cagaaattcc
    30361 cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg atcggcgcga
    30421 tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg attctgatca
    30481 gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg attcttggtc
    30541 tggtggccgg tattcagctt tccgcgaacg atatgctgaa actgggcgac tggctggaga
    30601 tgccgaaata cggcgcagat ggcgcggtga tcgatattgg gttaaccacc gtcaaagtgc
    30661 gtaactggga caataccatt accactattc ccacctggtc tctggtttct gactccttta
    30721 aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt atcagtattg
    30781 atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat aaagcgcatt
    30841 tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat cgccagcaag
    30901 gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc tttcgtgcct
    30961 atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc ttaatggtac
    31021 gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt actaacaccg
    31081 tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata tttgcgattg
    31141 tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt cgctctctgg
    31201 cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga gagcggacat
    31261 cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa atgccacaaa
    31321 caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat ccatcgtgca
    31381 catcagcagc aaaatcatgc cgaggaaggc aatgcagaga tagtttccga acggatagag
    31441 cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gctgcacgaa aacgcagatg
    31501 cgccagacag atcataatcc agttcaacag cagcgttgct accaccagcg ccatcagcag
    31561 accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag tgatcgctcc
    31621 ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca aaaacttcgg
    31681 cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa ccccgctgtt
    31741 atacactgac agcgatgcta ccagaatgac gaagttcagc gcagaagcta ccacgttgct
    31801 gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt ccacccacgg
    31861 atagagcgcc agtaaaacca ccagtgaacc gatgtaaaac agcaggatgc gatacaccac
    31921 ctgatttacc gcttttggaa tgcttttatc cggatcgcgc gcttcagcgg cagtaatccc
    31981 aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa tcagcccatt
    32041 ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg ctttctcgcc
    32101 gccgtgacca gaaaacagca gccacaggcc aaagccgatc ataccgatga ttgccagcac
    32161 tttaatcaac gcaaaccaga actcggtttc gccatataag cgcacgttca ccaggttaac
    32221 ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat ccgggaacca
    32281 gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca cgaacattac
    32341 ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat aggcaaagtg
    32401 ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc gcataatcag
    32461 gaaagcgatg atcccggcga cgccgtaacc cagcaataca gccggacccg ccatctgaat
    32521 cgccggacca atgccaagaa acagaccagt accaattgcg ccacccagcg caatcagttg
    32581 aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg cagtatcttc
    32641 cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt tgaggggcac
    32701 cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa cggggtatcc
    32761 tgcttttatt tccgtacccg atgtcggtgc agccacatca gcttatacgc cgccgggata
    32821 ataaacagcg acagcaaagg tgcggtgatc atgccgccaa tcatcggtgc ggcaatccgg
    32881 ctcatcacct ctgaaccagc ccccgttccc cacagaatcg gcagcagacc ggcgataatc
    32941 accgccaccg tcatcgcttt cgggcgcacg cgcagtaccg cgccgtgata caacgcctca
    33001 tccagcttct gctcactgaa tgtttgcgga ttattcaacg acggctcggc ctctatggcg
    33061 tgacgtaaat acatcagcat caccacgcca aattcggcgg cgaccccggc aagggcaata
    33121 aaaccggtgc ccgttgccac agaaagatga aagcccatcc accacagcaa ccagatgccg
    33181 cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgcccac ccgacggaat
    33241 gccagataca acagcacgaa gatgatcatc agtgtcatcg gcaccatgag tttcagctta
    33301 tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt gcccggtttt
    33361 aactgcactt tttcagctat cgctttttgc aaatcgtgaa ccaccgacac catgtcacga
    33421 tcgcgggcat caatataaat ccagctcgtc gggcgcgcat tctcggtttt cagcatcgac
    33481 ggtccggtag agactttaac gtcggccacg tctgccaggg tgatttgctg cttcatcggc
    33541 gtcaggatcg gtagctggcg cagtgcctgc gggctatcgc gccagctttg cggataacgc
    33601 agattaatgg gataacgggc aatcccttcc accgtttcgc caaccatcgc cccgcccacc
    33661 gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgcgc ggctttttca
    33721 cggttaatct caacgttgat atagcgccca ccttccagac gctcggctag agcagaagcc
    33781 acgcttggca ccgttcgcgc tacttcttca atttgctcag ccatcgcgtc gatatccgcc
    33841 agcacagtgc cggaaacttt aatgccgatg gggcttttaa tgccggttga gagcatatcg
    33901 atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagccg cacggtgtta
    33961 tccagttcct caatgatttt gtccatcgtc atgccaggcc gccactgatc ctgcggctta
    34021 agctggatgg tcgtttcaac catctcgagc ggagcagaat cggtggcggt ttccgctttc
    34081 ccggttttgc caaatacccg cgccacttca ggtacgctca taattagctt gtcggttttt
    34141 tgcagcatac tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg catatacaac
    34201 aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag cggccagaga
    34261 accgtcagca ccgaaagcgc tgccaccaac agcgtggttt tcggccagtg cagtactttc
    34321 agcaatagag gatgataaac acgaatcaaa aagcgattga gcgggttact gctttccggc
    34381 ggaattttgc cacggatcca gtaccccatc aggataggga tcactacgat cgccagcagc
    34441 gccgcgcccg ccatcgcata ggttttagtg aacgccaacg ggccaaacag acgcccttcc
    34501 tgcccttcca gggtgaagat cgggataaac gacaacgtga taatcagcag actgataaat
    34561 agcgccggac ccacttcaac agatgcatcg gtgatcacct gccagcgcgt tttattatcc
    34621 agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgtgc attttcgatc
    34681 atgacgatag cggcatcgac catcgccccg acggcaatcg caatgccgcc caacgacata
    34741 atattggcat tcagtccctg gaagtgcatg acaataaaag caatacacaa ccccagcggc
    34801 aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc acagactacc
    34861 gccaccacga taaactcttc cagcaacttg ccgctgaggt tgtcgatggc gcggtcaatg
    34921 agctggctgc gatcgtatgt tgtaactatc tccacgcctt ccggcagact acttttcagc
    34981 gtttccagtt tgtccttcac ggaggcgatc acttctcggg cgtttttgcc ggagcgcagg
    35041 atcaccaccc cgcccgccac ttcgccttcg ccattcagtt cggcaatgcc ccggcgcatc
    35101 tccgggccaa tctggacctt cgcaacatca cgcagataaa cgggcacgcc attttctctg
    35161 gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgca gatagccgct ggcgcgcacc
    35221 atatattccg cttccgccag ttcgatcgac gaaccgcccg cttcctggtt tgaagcatcc
    35281 agcgcgcttt ttacttcggc tagactgatg ccatattgcg ccaggcgctg gggatcgata
    35341 accacctgat actctttcac cacgccgccc accgacgcca cttccgcaac gtcagggatg
    35401 gtttttagct catatttgag aaaccagtcc tgcaatgagc gtaaatcggc cagatcgtgc
    35461 ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt ggcatctggt
    35521 cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag gtactccagc
    35581 acccgcgaac gcgcccagta cggatcggta ccatcttcga aaatgacata cacataagag
    35641 tctccaaact gcgagaaacc gcgcacagtc ttcgcgccag gcaccgacaa catggtggtg
    35701 gttagcggat aagtcacctg attttcaacg atttgcggtg cctgaccggg atagctggtt
    35761 ttaataatca cctgcacatc ggagagatcc ggcagcgcat ccactggcgt gttaatgatg
    35821 gtccaggtgc cccagatgct cagaaacaac gcgcccatca gcaccaaaaa acggttcgcc
    35881 accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca ttggtagcac
    35941 tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca atcaggaaca
    36001 ggccgctgga aaccaccttt tcaccttccg ccagaccaga gtgtaacgcg gtgacgcctt
    36061 gcgatgcctg gaaaacggca acgcgtttcg gtacaaagcg tccgtcggca tcaacggtaa
    36121 tcacccgctg ttcgttgccg gtatcaatca gcgcctgtga cggaatgagc agcatcggtt
    36181 cgctggcggt gttgagttgc aaccaggcgt tcatgcccgg ttttagcgcc tcgtcggcgt
    36241 tgtcgacttc cagacgcagc tgcagcgtgc gggtcgcggc atccacgcca ggtagcagcg
    36301 tccatttgcg gatggtgagt gttttatccg gtcgcgccgg aacggtgagc gtaaactgcg
    36361 aggcatcttt caccagccag gcgatagact ccgggatcgc agcagtgacc cacaccgggt
    36421 ccataccctg aattttcgct accacgttat ctttggcgat attcattccc gcgcgcagat
    36481 caaacgcggt gatcacgcca tcaatgggtg ctttgagcgt aaagcgagtc tggatttttt
    36541 gcgtggcgat caggcggcga atatccgcct ccggcattcc cgccagtcgc agccgctcaa
    36601 gaatgccttc agtctgggtc gccgtaccgc cggtttcgcg cagcagtaaa tactcactct
    36661 gcgcttccac ccagtcagga atggtcaggt cgagaagcgg tgtgcccttt tgtactttat
    36721 cgcccacggt aagcggatac accttgtcga taaacccggc agcgcgagcc tgcacaatgg
    36781 catactgata ctcgttgtaa ctgacattcg ccgggaaact ctgggcaaaa gtcagcggtc
    36841 cgcgcgtgac ggtagccgtt ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca
    36901 caccagacgc agaactctct tcatcggcat atttcggcac cagatccata tccataaacg
    36961 gcgatttacc tggtttatcg aaccgcgtat tggggtacat cgggtcgtac cagaataaga
    37021 ttttacgttc tgcggtcgac gttttttctg cgggtggttc agcctttgca aaccaggtaa
    37081 aacctgccgc agaaataata ccgcccgcga tcatgctgcc gataataagc gcgatttttt
    37141 tcatctcatt aaacctgggt tactggctga ctttaatatc ctgtaataaa gaaaggttgc
    37201 cctgctggac aaaattaaac gccactttgt cgccggtttt aatttcactc attttcgtct
    37261 gcggggtgat ggtaaagcgc atagtcatct ccggccagtt cacggcagca atcggatcgt
    37321 gatggatggt gatttttttg ctttccagat caataccctt taccacgcca gtggcgctaa
    37381 taacctgcgg ttgtacttcg ctcatggttt catgatgatg ttcgttagcc tgggcattaa
    37441 agccaataac ggtaaacaga ctgaacattg cgacttgcag tgcttttttc atttattctc
    37501 tcctggagtt aaaaattatt gctgccaacc gccccctaag gcggtataca aagaaatttc
    37561 gttaacctga cgggcataat tcaggtcgag taaagtttgt cgggttgcaa ataaagaacg
    37621 ctcggcatcc agcacttcca gataacttac cgcgccgtgc tgatataatg cccgcgcccg
    37681 ttgcaaagta atttgcagcg acgccagata acgctgctgg gcgctgattt gatcgttcag
    37741 gctttgacgt aatgccagcg catctgccac ttctttaaag gcgttctgga ttttctgttc
    37801 ataattcact accgactgct gctggcgaat ttcggcgata tccagattgg cctggttgcg
    37861 tccggcatta aaaatgggga tctcaatttt aggaataaaa ttccacatcc cgctgctagc
    37921 gttaaataat gaagacagat cgctgctggc ggttgatatt ccgctggtca ggctgataga
    37981 cgggaaaaat gccgcacgcg cagcaccaat attagcatta gccgccatta acgcgtgttc
    38041 agcttccata atatccgggc gctgcaataa gatttgcgac gacaagcccg ccggtaattt
    38101 aacgctttgc aggctgccgc tgtttactgt ctgcgcttgc ggcagtttgc cgtagcttcc
    38161 caataacagt tgcaatgcat tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc
    38221 actgcgggta ctttctatca ccccgcgagc ctgttccagc gccagaacat tgctgctacc
    38281 ggtcaacagt tgtttttcga caaacgcata tgactgctga taattacgca gcgtttcttc
    38341 ggctatttgc aattgcgcat acgccagttg ctgattgaaa tagctttgcg cgacattaga
    38401 aaccagcaga atatgcaccg cgcgctgagc ttcctcagtg gctaaataat tttgtcgctc
    38461 ggcttcgctc atgttcttta agcgaccgaa aaaatcgagg tcaaagctgg cgttaaggcc
    38521 ggtcgaaaac tcccgtgtcg tggcggtatt ccctttaaga ttaccgctcc agctgccgct
    38581 gccctcgcca ttgagctgtg ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc
    38641 ctcctgcact ttcagcgtcg ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat
    38701 cagcgtcttc acctgattat caacaaaaaa ggtgcgccag cccacgttct ggtagttatc
    38761 tgctgcgtta accaagccgt tctggctgag tgagaactgc tgcggcacgg gcattgccgt
    38821 acgctgataa tccggtgcca gtgaacaacc ggttagcgca agggccacac aaaatggcag
    38881 aagtttacaa ggagacatag gctcataatt tctggtgatt ttataccgcc aactttactc
    38941 gccaggctct gattttccgg tgacaggaaa atgacaaaat tgtcattttg ccaataagcg
    39001 attgccatct gatcccgcta ctctagaatt gcccgggcaa catgcggagg aaaaatgaaa
    39061 ctgttgattg tcgaagatga aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa
    39121 gccggttttg tggtcgattt ggccgacaac gggctgaatg gctaccatct ggcgatgacc
    39181 ggtgattatg acctgataat cctcgatatt atgctgccgg acgtgaacgg ctgggatatc
    39241 gtgcgcatgt tgcgctccgc caataaaggg atgccgattc tgttgcttac cgcgcttggc
    39301 accattgaac atcgcgtcaa ggggctggag ttgggggccg atgactatct ggtgaagccg
    39361 ttcgcttttg ctgaactgct ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg
    39421 attatcgaaa gtcagtttca ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc
    39481 acccgcagcg gcacgcgcat cactctgacc agtaaagagt ttactctgct ggagtttttt
    39541 ctccgccatc agggcgaagt gctgccccgc tcgcttatcg cctcgcaggt gtgggacatg
    39601 aatttcgaca gcgacactaa cgccattgat gtggcggtga agcggctacg cggcaaaatc
    39661 gacaacgact ttgagccgaa gctgattcag accgtgcgcg gcgtgggcta tatgcttgag
    39721 gtgccggatg gtcagtaagc catttcagcg cccgttttcg ctggcaaccc gtctgacctt
    39781 ttttatcagc ctggccacca tcgcggcgtt tttcgccttt gcatggatca tgatccactc
    39841 agtgaaagtg cattttgccg agcaggatat taatgattta aaagaaatta gcgccaccct
    39901 tgaacgggta cttaatcatc ctgacgagac gcaagcccga cgcttaatga cgctggaaga
    39961 tatcgtcagt ggttattcca acgtgttgat ttccctggca gatagccacg gcaaaacggt
    40021 gtatcactcc cccggtgcgc cggatatccg cgagtttacg cgtgacgcca tacccgataa
    40081 agacgctcag ggtggcgagg tgtatctcct ttccggcccg acgattatga tgccaggcca
    40141 cggtcacggg catatggaac acagcaactg gcggatgatt aacttgccgg ttggcccgtt
    40201 ggtggacggc aaaccgattt atacgctcta catcgcgctt tcgatcgatt ttcatcttca
    40261 ttacataaat gatttgatga ataaacttat tatgaccgca tctgtcatca gcatcctgat
    40321 cgtctttatc gtgctattgg cggtacataa aggtcacgcg ccgatccgca gcgtcagccg
    40381 tcaaatccag aatatcacct cgaaagatct cgacgttcgc cttgacccgc agaccgagcc
    40441 cattgagctg gaacagctgg tactgtcgtt caaccatatg atcgagcgta ttgaggatgt
    40501 ctttacccgc cagtccaatt tctcagcgga tatcgctcac gaaattcgca cgccgattac
    40561 caatctcatt acgcaaacgg aaatcgccct cagccagtct cgcagccaga aggagctgga
    40621 agatgtgctc tactctaatc tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat
    40681 gctgtttctc gctcaggccg ataacaacca gctaatcccc gaaaagaaaa tgctcaacct
    40741 ggcggatgaa gtcagcaaag tgttcgattt tttcgaggcg ttagcggaag atcgcggcgt
    40801 ggagttgcga tttgttggcg acaaatgtca ggttgcgggc gatccgctga tgctgcgtcg
    40861 ggcgttaagc aacctgcttt ctaatgccct gcgttatacg ccaaccagag agacaattgt
    40921 agtgcgctgt cagacggtcg atcaccaggt gcaagttacc gtcgaaaacc ccggtacgcc
    40981 cattgcgccc gagcacttac cgcgattgtt tgaccgtttc taccgcgttg acccttcccg
    41041 ccagcgaaaa ggcgaaggta gcggcattgg gctggcgata gtgaaatcga ttgttgtcgc
    41101 gcataaaggc acggttgcag taacgtcaga tgcgcggggg acaaggtttg tgattatgct
    41161 gccggagaga gagtgattat taagttcaaa ttcagggcgt ggtgcatcac ctcctggtat
    41221 tgttgatcaa caaattagag aaaaacttat taaacatatg aaatatcgaa ggcttttatt
    41281 tttcaaagtt tcacgagatg tttgtatatg aaacttagtt aaatctgtgt ttcttccaat
    41341 tgtagatcac aatgcccttt ctaaatt
//
LOCUS       DAZPAC010000012        41188 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZPAC010000012 DAZPAC010000000
VERSION     DAZPAC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.012"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(35..1102)
                     /gene="degS"
                     /locus_tag="ACHUPM_000523"
     CDS             complement(35..1102)
                     /gene="degS"
                     /locus_tag="ACHUPM_000523"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR0868405.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(1192..2559)
                     /gene="degQ"
                     /locus_tag="ACHUPM_000524"
     CDS             complement(1192..2559)
                     /gene="degQ"
                     /locus_tag="ACHUPM_000524"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFR0868406.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            complement(2713..3111)
                     /gene="zapG"
                     /locus_tag="ACHUPM_000525"
     CDS             complement(2713..3111)
                     /gene="zapG"
                     /locus_tag="ACHUPM_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR0868407.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            3305..4432
                     /gene="zapE"
                     /locus_tag="ACHUPM_000526"
     CDS             3305..4432
                     /gene="zapE"
                     /locus_tag="ACHUPM_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR0868408.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            4651..5079
                     /gene="rplM"
                     /locus_tag="ACHUPM_000527"
     CDS             4651..5079
                     /gene="rplM"
                     /locus_tag="ACHUPM_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR0868409.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            5095..5487
                     /gene="rpsI"
                     /locus_tag="ACHUPM_000528"
     CDS             5095..5487
                     /gene="rpsI"
                     /locus_tag="ACHUPM_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR0868410.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            5882..6520
                     /gene="sspA"
                     /locus_tag="ACHUPM_000529"
     CDS             5882..6520
                     /gene="sspA"
                     /locus_tag="ACHUPM_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR0868411.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMCLGRS"
     gene            6526..7023
                     /gene="sspB"
                     /locus_tag="ACHUPM_000530"
     CDS             6526..7023
                     /gene="sspB"
                     /locus_tag="ACHUPM_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR0868412.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(7066..8388)
                     /gene="dcuC"
                     /locus_tag="ACHUPM_000531"
     CDS             complement(7066..8388)
                     /gene="dcuC"
                     /locus_tag="ACHUPM_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0868413.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            8768..9559
                     /gene="nanR"
                     /locus_tag="ACHUPM_000532"
     CDS             8768..9559
                     /gene="nanR"
                     /locus_tag="ACHUPM_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR0868414.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            9681..10574
                     /gene="nanA"
                     /locus_tag="ACHUPM_000533"
     CDS             9681..10574
                     /gene="nanA"
                     /locus_tag="ACHUPM_000533"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR0868415.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            10684..12174
                     /gene="nanT"
                     /locus_tag="ACHUPM_000534"
     CDS             10684..12174
                     /gene="nanT"
                     /locus_tag="ACHUPM_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR0868416.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            12222..12911
                     /locus_tag="ACHUPM_000535"
     CDS             12222..12911
                     /locus_tag="ACHUPM_000535"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR0868417.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            12908..13783
                     /gene="nanK"
                     /locus_tag="ACHUPM_000536"
     CDS             12908..13783
                     /gene="nanK"
                     /locus_tag="ACHUPM_000536"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR0868418.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            13780..14244
                     /gene="nanQ"
                     /locus_tag="ACHUPM_000537"
     CDS             13780..14244
                     /gene="nanQ"
                     /locus_tag="ACHUPM_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR0868419.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(14320..14625)
                     /locus_tag="ACHUPM_000538"
     CDS             complement(14320..14625)
                     /locus_tag="ACHUPM_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0868420.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(14717..15121)
                     /locus_tag="ACHUPM_000539"
                     /pseudo
     CDS             complement(14717..15121)
                     /locus_tag="ACHUPM_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            complement(15305..16723)
                     /gene="gltD"
                     /locus_tag="ACHUPM_000540"
     CDS             complement(15305..16723)
                     /gene="gltD"
                     /locus_tag="ACHUPM_000540"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR0868421.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(16736..21196)
                     /gene="gltB"
                     /locus_tag="ACHUPM_000541"
     CDS             complement(16736..21196)
                     /gene="gltB"
                     /locus_tag="ACHUPM_000541"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR0868422.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            21871..22800
                     /locus_tag="ACHUPM_000542"
     CDS             21871..22800
                     /locus_tag="ACHUPM_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR0868423.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTFTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            22896..25232
                     /gene="arcB"
                     /locus_tag="ACHUPM_000543"
     CDS             22896..25232
                     /gene="arcB"
                     /locus_tag="ACHUPM_000543"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR0868424.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            25462..26115
                     /gene="elbB"
                     /locus_tag="ACHUPM_000544"
     CDS             25462..26115
                     /gene="elbB"
                     /locus_tag="ACHUPM_000544"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR0868425.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            26112..26840
                     /gene="mtgA"
                     /locus_tag="ACHUPM_000545"
     CDS             26112..26840
                     /gene="mtgA"
                     /locus_tag="ACHUPM_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFR0868426.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(26837..27469)
                     /gene="yrbL"
                     /locus_tag="ACHUPM_000546"
     CDS             complement(26837..27469)
                     /gene="yrbL"
                     /locus_tag="ACHUPM_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR0868427.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27683..27955)
                     /gene="npr"
                     /locus_tag="ACHUPM_000547"
     CDS             complement(27683..27955)
                     /gene="npr"
                     /locus_tag="ACHUPM_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR0868428.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(27952..28806)
                     /gene="rapZ"
                     /locus_tag="ACHUPM_000548"
     CDS             complement(27952..28806)
                     /gene="rapZ"
                     /locus_tag="ACHUPM_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR0868429.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(28852..29343)
                     /gene="ptsN"
                     /locus_tag="ACHUPM_000549"
     CDS             complement(28852..29343)
                     /gene="ptsN"
                     /locus_tag="ACHUPM_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR0868430.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            complement(29461..29748)
                     /gene="hpf"
                     /locus_tag="ACHUPM_000550"
     CDS             complement(29461..29748)
                     /gene="hpf"
                     /locus_tag="ACHUPM_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR0868431.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(29771..31204)
                     /gene="rpoN"
                     /locus_tag="ACHUPM_000551"
     CDS             complement(29771..31204)
                     /gene="rpoN"
                     /locus_tag="ACHUPM_000551"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR0868432.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(31252..31977)
                     /gene="lptB"
                     /locus_tag="ACHUPM_000552"
     CDS             complement(31252..31977)
                     /gene="lptB"
                     /locus_tag="ACHUPM_000552"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR0868433.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(31984..32541)
                     /gene="lptA"
                     /locus_tag="ACHUPM_000553"
     CDS             complement(31984..32541)
                     /gene="lptA"
                     /locus_tag="ACHUPM_000553"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR0868434.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(32510..33085)
                     /gene="lptC"
                     /locus_tag="ACHUPM_000554"
     CDS             complement(32510..33085)
                     /gene="lptC"
                     /locus_tag="ACHUPM_000554"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR0868435.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(33082..33648)
                     /gene="kdsC"
                     /locus_tag="ACHUPM_000555"
     CDS             complement(33082..33648)
                     /gene="kdsC"
                     /locus_tag="ACHUPM_000555"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR0868436.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(33669..34655)
                     /gene="kdsD"
                     /locus_tag="ACHUPM_000556"
     CDS             complement(33669..34655)
                     /gene="kdsD"
                     /locus_tag="ACHUPM_000556"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR0868437.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            complement(34669..35646)
                     /locus_tag="ACHUPM_000557"
     CDS             complement(34669..35646)
                     /locus_tag="ACHUPM_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR0868438.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            35856..36665
                     /gene="mlaF"
                     /locus_tag="ACHUPM_000558"
     CDS             35856..36665
                     /gene="mlaF"
                     /locus_tag="ACHUPM_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR0868439.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            36673..37455
                     /gene="mlaE"
                     /locus_tag="ACHUPM_000559"
     CDS             36673..37455
                     /gene="mlaE"
                     /locus_tag="ACHUPM_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR0868440.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLANCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            37460..38011
                     /gene="mlaD"
                     /locus_tag="ACHUPM_000560"
     CDS             37460..38011
                     /gene="mlaD"
                     /locus_tag="ACHUPM_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR0868441.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            38030..38665
                     /gene="mlaC"
                     /locus_tag="ACHUPM_000561"
     CDS             38030..38665
                     /gene="mlaC"
                     /locus_tag="ACHUPM_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR0868442.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            38665..38958
                     /gene="mlaB"
                     /locus_tag="ACHUPM_000562"
     CDS             38665..38958
                     /gene="mlaB"
                     /locus_tag="ACHUPM_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR0868443.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            39118..39372
                     /gene="ibaG"
                     /locus_tag="ACHUPM_000563"
     CDS             39118..39372
                     /gene="ibaG"
                     /locus_tag="ACHUPM_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR0868444.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            39427..40686
                     /gene="murA"
                     /locus_tag="ACHUPM_000564"
     CDS             39427..40686
                     /gene="murA"
                     /locus_tag="ACHUPM_000564"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR0868445.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(40734..41012)
                     /gene="sfsB"
                     /locus_tag="ACHUPM_000565"
     CDS             complement(40734..41012)
                     /gene="sfsB"
                     /locus_tag="ACHUPM_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR0868446.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
BASE COUNT         9640 a        10469 c        10961 g        10118 t
ORIGIN      
        1 cacagactcc ggttttttgt tttgagcact cgacttaatt ggttgccgga tattcctgaa
       61 tggtgacctg cagcgttaac tgcttatcat cacgcatcac tacaaccggg atcaccgaac
      121 cagggcgaat ttccgccacc tgatccatcg tctccagagc agagatggcc ggtttgttat
      181 ccaccgaaat aatcagatcg ttgacctgaa tacccgcatt cgccgccggg ccgtcaggtg
      241 acacttcatt aaccacgatc ccttgcagtt gatctatacc accgccctgt gcgtgcagcg
      301 gtgcgatctc acgtccgcca atgccaatgt agccgcggat cacgcgacca tcgcggatca
      361 gcttatccat aattttggtt gctaactgga aaggaatcgc aaagccgata ccttccggcg
      421 tttcgccatc gttactctta tcaaacgaca gcgtgttaat gcccatcagt tcgcccagcg
      481 agttcaccag cgcgccgcca gagttaccgt ggttaatgga agcatcggtt tgcaggaagt
      541 tttgccgccc ggtcgggttc agaccaattc gacccgtggc actaataatc ccctgggtaa
      601 tggtctgccc gaggttgtac gggttaccga tcgccagtac cacgtcgcca atatgcggta
      661 cgcgacgtgc attgattgga atggtaggta aaccgccagt ggcattaatt ttaagtaccg
      721 ccagatcggt tagagagtca gatcccacca gcaatgcttc aaatacacga ccatcctgta
      781 aggcgacgat gatctgatcg gcgtcgttga tgacgtgttt attggtgatg atataaccgc
      841 gttgatccat gattacaccg gatcccaggg tgcggatctc aagctggttg tgagagttgg
      901 tgttcaaacc acggttgtaa acgttaacca ccgctggcgc ggcgcggcga accgccagat
      961 tatagctggc aggcgtctca tcggtactgt caaattgcgg agtggaaagc gggttaaggc
     1021 tgcgcagcga aggcatggca accagcagaa tagcgccgac aattaatcca atcgcaacgg
     1081 aacgtaagag cttcacaaac atgatggagg cgtcattaaa aaagggaacg gcagcagcat
     1141 accacgagtt aaccggacat cacacgtaag cctgatgccc ggtttacgac attaacgcat
     1201 cagcagatag atgctttcat tgccgcgtac aatttgcagg gcgatgatgg ccgattttgc
     1261 cgccagcact ttacgcattt cagcaatcga gttcacccga tcgcggttga cgccaatgat
     1321 cacatcgtct ttttgcaagc cagcctgagc agctgggctt cctttgacaa cttcatcgat
     1381 cttaatacct ttgccgccat cttttagctg accatcgctc aacgttgcac cttccagcgc
     1441 tggcgcgatc atttcagcgc tggccgacga agaggtgctg gtatcgagcg tcacttctac
     1501 ttccagtggt ttgccgttac gcagcaggcc aagtttcact tttgtgcccg gctcggtggt
     1561 cgcgatacga gagcgcaact cagcaaagct attcagcggt ttgccgttga ggctggtaat
     1621 aatatcgccc gctttgacgc ccgctttcgc cgagccagaa cctggcaaca cttcgctgac
     1681 aaacgcgcca cgctgcacgt caaggttgaa ggctttggcg atatcggcac tcatctcggt
     1741 gcctttgatg cctaacaaac cgcgtttgat ttcaccaaag tcgataagct gctgcgccag
     1801 tgttcgcgcc atattactgg ggatggcaaa tccaatcccg acgctcccgc cgccaggcgc
     1861 aaggattgca gtgttgatgc caattaactc accgttaagg tttaacagtg caccgccgga
     1921 gttaccgcgg ttaatggaag catctgtctg gataaagttt tccagacctt caagattcaa
     1981 cccgctgcgt cctaatgcgg aaacaatgcc agaggtggcg gtttgcccaa ggccaaatgg
     2041 gttaccgacc gctacggcaa aatcaccgac gcgcaattta tcggagtcgg caatagcgat
     2101 ttgcgttaat ttgctcgggt tttgaatttg taacagggcg atatcgctct ggtcatcgct
     2161 accaatcagt tttgcatcaa actcgcgccc atcattgagc tgaatactga ttttctgtgc
     2221 ctgattaatc acatggttgt tggtcagcac atagccttta ctggcgttga tgatgacacc
     2281 ggagcctaaa ccttcgaagg gttgtgcagg ttgctccggt aaatcatcac caaaaaactt
     2341 tttgaattct tccgggattt tctgtccctg actggccgtt ccttccaccc gtacgctcac
     2401 cactgccgga agcacttttt ccagcattgg agccagactg gggagagggg cctgatcggc
     2461 aacctggcct ggaatcgacg cgacggcctg aaatgacgcc gagagagtta acccgacact
     2521 taacgctaat gcactcaaca gctggggttg ttttttcatt attcctgctc tcgtacctga
     2581 atgataagaa aagagattca aaacagtttg atgttattga attttcagac ggctaacaat
     2641 gagacaccag attaaatgat agtcggggag ggagagaaga ggagggcgca atggctgcgc
     2701 ccgaaaaata aattagtcgc gcttcgcgcc agtacgcagc aggccggatg cgccttcaga
     2761 atagtcgcga ggcatctgca ccggtgcctg atcgttgctg gcttcagact ctgccagacg
     2821 attacggaac gggtttgctt cagcagacag ttccggcagc aggctgctgg aacttttcgc
     2881 catgtgctga tacagctggc gatagtcgtg cgccatggta tccagtaatt ccgcgctgcg
     2941 ggcaaagtgg ctaaccagct cttcgcgata ctcgtccagt tcagctttat tcttttccag
     3001 ttcgtactgc aacgcctgtt gctggcgtag tttacgatta ccaaaacgca tggccacagc
     3061 accaataatg atgccgacga ctaacccaat tagcgcatat tcccaggtca tgaacatctc
     3121 ccgttgtctt ttgtttccgt agggtgttgg cttcaggctc catgcctgcg gctgattatg
     3181 ccactataac cgttaattcc acagaagtgg aatcccgact gcatatcgcg tagtgtagaa
     3241 cggccttttt ttcgtcaacc gtgaacaacg gcgcaccgat tattcaagga ataacaataa
     3301 gatcatgcaa agcgttaccc caacatcgca atacctgaag gcgctcaatg aaggcagcca
     3361 tcaacccgac gacgttcaaa aagaggccgt cagccgcctg gaaattattt atcaggaact
     3421 catcaatagc acgccaccag cccccaggac gagtgggcta atggcgcggg tcggtaagct
     3481 gtggggtaaa cgcgaagaca caaagcatac gccagtgcgt ggcttatata tgtggggcgg
     3541 tgtaggacgc gggaaaacct ggctgatgga ccttttctat caaagcctgc cgggagagcg
     3601 gaaacagcgc ctgcactttc accgttttat gctgcgggtg catgaagagc taactgcctt
     3661 acaggggcag accgatccgc tggaaattat tgccgatcgc tttaaagccg aaactgacgt
     3721 gctctgtttt gacgaatttt ttgtttctga tattaccgat gccatgctac ttggcggtct
     3781 gatgaaagcc ttgttcgccc gcggtattac cctggtagcg acgtcaaata ttccgccgga
     3841 cgaactttat cgaaatggct tgcaacgtgc gcgttttctg cctgcaatcg atgccattaa
     3901 acagcattgt gatgtaatga acgtggacgc tggtgttgat tatcgactgc gtacactcac
     3961 tcaggcgcat ctgtggcttt cgccactcca cgatgaaacc cgggcgcaaa tggataaact
     4021 atggttggcg ctggcggggg ggaaacgaga aaattcaccg acgttagaaa tcaaccatcg
     4081 gccattagcg acaatgggcg tcgagaacca gacgctggcg gtctctttta ctacgctgtg
     4141 cgtcgacgcc cgcagtcagc atgactatat tgcgctctca cgtctctttc atacggtcat
     4201 gttgtttgat gtaccagtta tgacgcggtt gatggagagc gaagcgcggc gctttattgc
     4261 gctggtggat gagttttacg agcgccatgt caaattagtg gtgagtgcag aagtgccgct
     4321 gtatgaaatt tatcagggcg atcggctgaa gtttgagttc cagcgttgcc tgtcacgtct
     4381 gcaagagatg caaagcgaag agtatctgaa gcgcgagcat ttggcgggtt aaaacctgtc
     4441 acaaatcaca aaaaggggtc gatctttgac cccgacttct ctataatcct gcgaccccac
     4501 gttacaagaa agtttttttc ccaaaacttt ttgtgtgctg gcataggcta ttcgaagggg
     4561 taggtttgcc ggactttgtc gtgtgaacct caacaattga agacgtttgg gtgttcacca
     4621 acgtgtaact atttattggg taagctttta atgaaaactt ttacagctaa accagaaacc
     4681 gtaaaacgcg actggtatgt tgttgacgcg accggtaaaa ctctgggccg tctggctact
     4741 gaactggctc gtcgcctacg cggtaagcac aaagcggaat acactccgca cgtagatacc
     4801 ggtgattaca tcatcgttct gaacgctgac aaagttgctg taaccggcaa caagcgtact
     4861 gacaaagtgt actatcacca caccggccac atcggtggta tcaaacaagt gagctttgaa
     4921 gagatgattg ctcgccgtcc tgagcgtgtg attgaaatcg cggttaaagg catgttgcca
     4981 aaaggcccgc tgggtcgtgc tatgttccgt aaactgaaag tttacgcggg taacgagcac
     5041 aaccacgcgg cacagcaacc gcaagttctt gacatctaat cgggattata ggcaatggct
     5101 gaaaatcaat actacggcac tggtcgccgc aaaaattccg cagctcgcgt tttcatcaaa
     5161 ccgggcaacg gtaaaatcgt aatcaaccaa cgttctctgg aacagtactt cggtcgtgaa
     5221 actgcccgca tggtagttcg tcagccgctg gaactggtcg acatggttga gaaactggac
     5281 ctgtacatca ccgttaaagg tggtggtatc tctggtcagg ctggtgcgat ccgtcacggt
     5341 atcacccgcg ctctgatgga atacgacgag tccctgcgtt ctgaactgcg taaagctggc
     5401 ttcgttactc gtgacgctcg tcaggttgaa cgtaagaaag tcggtctgcg taaagcacgt
     5461 cgtcgtccgc agttctccaa acgttaattg gcttctgctc cggcagaaaa caatttacga
     5521 aaaaacccgc ttcggcgggt ttttttatgg ctaaattctg aatcagcgta aaaaatggga
     5581 agttgctttt tgctgccgtc tgaaagacag gcaaaacaaa aaccacatcg ccaataaggg
     5641 tctaagtcaa ctatttcaga ctaaagcgca tctctttttc cccatttccg gcatccactc
     5701 accacaatgg tcgcaaaatc tggtaaacta tcatccaatt ttctgcccaa atgtcgggta
     5761 ttgctcattt tttgtttgat tttcgaacaa agagagtcgt tccttattgg gtaacacaac
     5821 ttctgactgg ccacctggtg gctggtagca gtaaaaattc tgactatacc tggaggtttt
     5881 catggctgtc gctgccaaca aacgttcggt aatgacgctg ttttccggtc ctactgacat
     5941 ctatagccat caggtccgca ttgtgctggc tgagaaaggt gtaagtttcg agatcgaaca
     6001 cgtggaaaag gacaatccgc ctcaggatct gattgacctc aacccgaatc agagcgttcc
     6061 gaccctggtg gatcgtgagc tgaccctgtg ggaatctcgc atcattatgg aatatctgga
     6121 tgagcgtttc ccgcatccgc cactgatgcc tgtttacccg gtagctcgcg gtgaaagccg
     6181 tctgtacatg catcgcatcg aaaaagactg gtacacgctg atgaacacca tcatcaacgg
     6241 ttcagcttct gaagcagatg ccgcacgtaa gcaactgcgc gaagaactgc tggcgattgc
     6301 gccggtcttc ggtcagaagc cgtacttcct gagcgatgag ttcagcctgg tcgattgcta
     6361 tcttgctccg ctgctgtggc gtctgccgca actgggtatc gagctcagcg gcccgggtgc
     6421 gaaagagctg aaaggctata tgacccgcgt ctttgagcgt gactctttcc ttgcttcttt
     6481 aactgaagca gaacgtgaaa tgtgtctggg ccggagttaa tctgtatgga tttgtcacag
     6541 ctaacaccac gtcgtcccta tctgctgcgt gcattctatg agtggttgct ggataaccag
     6601 ctcacgccgc acctggtggt ggatgtgacg ctccctggcg tgcaggttcc tatggaatat
     6661 gcgcgtgacg ggcaaatcgt actcaacatt gcgccgcgtg ctgtcggcaa tctggaactg
     6721 gcgaatgatg aggtgcgctt taacgcgcgc tttggtggca ttccgcgtca ggtttctgtg
     6781 ccgctggctg ccgtgctggc tatctacgcc cgtgaaaatg gcgcaggcac gatgtttgag
     6841 cctgaagctg cctacgatga agataccagc atcatgaatg atgaagaggc atcggcagac
     6901 aacgaaaccg ttatgtcggt tattgatggc gacaagccag atcacgatga tgacactcat
     6961 cctgacgatg aacctccgca gccaccacgc ggtggtcgac cggcattacg tgttgtgaag
     7021 taatacaaaa caggcccagg cggcctgttt tgtcttttta atgacttaat aaaatagcgt
     7081 gattgtggca atcatattca ccacgaaacc gactgccatg gggatcgctg tccgcttcac
     7141 caccgcagta atcggcgaaa ccgcgcgcgc tagcgtcgtg gcaaaatgca tcggcataat
     7201 cattacaacc gctggtacat gtagtccggc tgcgatattc ggaataagac tggcaaatga
     7261 cataaacggc gcattgccag agcccatcac aatggcacaa atggcaatga ctagcgccat
     7321 aataatcatc acgccaatac cgcccagacc agaatgctcc gcacccctga taaccgcatc
     7381 aacagtgcca atcgtcgtta agcctttcgc aaatatttca cccgcgacca ccagcgttac
     7441 cacgttggca aactgcgtac ccatgccgtc aaaaaacgcc tgcacatcgt ccattgtttc
     7501 gcgcaagtta tgcttgcgga agaactcaac aaacatcgtc accgtcaaac ttagtaacat
     7561 caccaccacc agatgcagtt ctgacttcat cagcccgacg tgggcgagga acagcgagcc
     7621 gagcatcagg attaacggca tcacaggtaa aatggcgtaa tagagcggcg ggacattatc
     7681 gagagttttt tgctctgcct gttcgtgatt gatattttta tcttttttgt caaaagcgcg
     7741 ttgcacgaaa aagtgggaga tcgccaccga gataatgacg caagaggcga ccggaagctg
     7801 gtagtggaag aagtatgtgg caattttcat tcccgcgacc tgggctgcaa aaatggagtt
     7861 cgtttccaga atcccccact caatggacat cgtggttgcg ataaccgcca ccgcagagag
     7921 gcgacttact cccagactca ccagcgtcgg aaataaggtg accatcagca acatacccag
     7981 accggaggcg ctggtaataa actgcgccat gatttggccg atgacgtaag ttgccgacag
     8041 aataatatac ggcgagcgaa tgagttttaa cgggcggctt agcaagctca ccatcgcgcg
     8101 actggctcct atgcgctcca tgtagcgggc ataaccgccc accgccataa tcgacagccc
     8161 caatccggca atgcggttgg acagcatgcg caggatttca ttataaatgt cgacgatcaa
     8221 gtagccgctg cttttggcgg gatcgagtac accaccgaac cctaaccaga ctccgcacat
     8281 catcaggaag acacctgcgg cagccagcac cacctgaggt ttgtagtttt tcaggatcaa
     8341 atagcccatc gtaattaata cgatgacaga tataattatg ccgaacattg ttgaactccg
     8401 tgtcaaaaga aaacggtcaa tcccataaac ggcagattga aaacaatgat gttatatttt
     8461 ttgcaagtct atttatggtg cggatgtcgt gtttttaatt gtaggtgatg tgatttttca
     8521 ttaaaaaata tgcgcttatg attattttgt aagaacacat tcataatatt cataatgctt
     8581 gtgaatagtc ttatgaataa ttcaatcggg atgtgtttat ctgcgttaca ttaatttttc
     8641 gcaatagtta attattccgt taattatggt aatgatgagg cacaaagaga aaaacctgcc
     8701 attttcccct actttcaatc ctgtgatagg atgtcactga tgatgttaat cacactgacc
     8761 ttacagaatg ggccttatga acgcatttga ttcgcaaacc gaagattctt cacctgcaat
     8821 tggtcgcaac ttgcgtagcc gcccgctggc gcgtaaaaaa ctctccgaaa tggtggaaga
     8881 agagctggaa cagatgatcc gccgtcgtga atttggcgaa ggtgaacaat taccgtctga
     8941 acgcgaactg atggcgttct ttaacgtcgg gcgtccttct gtgcgcgaag cgctggcagc
     9001 gttaaaacgc aaaggtctgg tgcaaataaa caacggcgaa cgcgctcgcg tctcgcgtcc
     9061 ttctgcggac actatcatcg gtgagttttc cggcatggcg aaagatttcc tttctcatcc
     9121 cggtgggatt gcccatttcg aacaattacg tctgttcttt gaatccagtc tggtgcgcta
     9181 tgcggctgaa catgccaccg atgagcaaat cgatttgctg gcaaaagcac tggaaatcaa
     9241 cagtcagtcg ctggataaca acgcggcatt cattcgttca gacgttgatt tccaccgcgt
     9301 gctggcggag atccctggta acccaatctt catggcgatc cacgttgccc tgctcgactg
     9361 gctgatcgcc gcacgtccaa ccgtggctga tcaggctctg cacgagcata acaacgttag
     9421 ctatcaacag catattgcga tcgttgatgc gatccgccgt catgatcctg acgaagccga
     9481 tcgtgcgttg caatcgcatc tcaacagcgt ctctgctacc tggcacgctt tcggtcagac
     9541 caccaacaaa aagaaataat gccactttag tgaagcagat cgcattataa gctttctgta
     9601 tggggtgttg cttaattgat ctggtataac aggtataaag gtatatcgtt tatcagacaa
     9661 gtatcacttc agaggtattt atggcaacga atttacgtgg cgtaatggct gcactcctga
     9721 ctccttttga ccaacaacaa gcactggata aagcgagtct gcgtcgcctg gttcagttca
     9781 atattcagca gggcatcgac ggtttatacg tgggtggttc gaccggcgag gcctttgtac
     9841 aaagcctttc cgagcgtgaa caggtactgg aaatcgtcgc cgaagaggcg aaaggtaaga
     9901 ttaaactcat cgcccacgtc ggttgcgtca gcaccgccga aagccaacaa cttgcggcat
     9961 cggctaaacg ttatggcttc gatgccgtct ccgccgtcac gccgttctac tatcctttca
    10021 gctttgaaga acactgcgat cactatcggg caattattga ttcagcggat ggtttgccga
    10081 tggttgtgta caacattcca gccctgagtg gggtaaaact gaccctggat cagatcaaca
    10141 ccctggttac gttgcctggc gtcggtgcgc tgaaacagac ttctggcgat ctctatcaga
    10201 tggaacagat ccgtcgtgaa catcctgatc tggtgctcta taacggttac gacgaaatct
    10261 tcgcttccgg cttgctggca ggcgctgatg gtggtatcgg cagtacttac aacatcatgg
    10321 gctggcgata tcaggggatc gtgaaggcgc tgaaagaagg cgatatccag accgcgcaga
    10381 aactgcaaac tgagtgcaac aaagtcattg atttactgat caaaactggc gtattccgcg
    10441 gcctgaaaac tgtcctgcat tatatggatg tcgtttctgt gccgctgtgc cgcaaaccgt
    10501 ttggaccggt agatgaaaaa tatctgccag aactgaaggc gctggcccag cagttgatgc
    10561 aagagcgcgg gtgagttgtt ttcccctcgc tcgcccctac cgggtgaggg gaaataaacg
    10621 catctgtacc ctacaatttt cataccaaag cgtgtgggca tcgcccaccg cgggagactc
    10681 acaatgagta ctacaaccca gaatatcccg tggtatcgcc atctcaaccg tgcacaatgg
    10741 cgcgcatttt ccgctgcctg gttgggatat ctgcttgacg gttttgattt cgttttaatc
    10801 gccctggtac tcaccgaagt acagggtgaa ttcgggctga cgacggtgca ggcggcaagt
    10861 ctgatctccg cagcctttat ctctcgctgg ttcggcggcc tgatgctcgg cgctatgggt
    10921 gaccgctacg ggcgtcgtct ggcaatggtc accagcatcg ttctcttctc ggccgggacg
    10981 ctggcctgcg gctttgcgcc aggctacatc accatgttta tcgctcgtct ggtcatcggc
    11041 atggggatgg cgggtgaata cggttccagc gccacctatg tcattgaaag ctggccaaaa
    11101 catctgcgta acaaagccag tggttttttg atttcaggct tctctgtggg ggccgtcgtt
    11161 gccgctcagg tctatagcct ggtggttccg gtctggggtt ggcgtgcgct gttctttatc
    11221 ggcattttgc caatcatctt tgctctctgg ctgcgtaaaa acatcccgga agcggaagac
    11281 tggaaagaga aacacgcagg taaagcacca gtacgcacaa tggtggatat tctctaccgt
    11341 ggtgaacatc gcattgccaa tatcgtaatg acactggcgg cggctactgc gctgtggttc
    11401 tgcttcgccg gtaacctgca aaatgccgcg atcgtcgctg ttcttgggct gttatgcgcc
    11461 gcaatcttta tcagctttat ggtgcagagt acaggcaaac gctggccaac gggcgtaatg
    11521 ctgatggtgg tcgtgttgtt tgctttcctc tactcatggc cgattcaggc gctgctgcca
    11581 acgtatctga aaaccgatct ggcttataac ccgcatactg tcgccaatgt gctgttcttt
    11641 agtggctttg gcgcggccgt gggatgctgc gtaggaggct tcctcggtga ctggctggga
    11701 acccgcaaag cgtacgtttg tagcctgctg gcctcgcagc tgctgattat tccggtattt
    11761 gcgattggcg gcgcaaacgt ctgggtgctc ggtctgttac tgttcttcca gcaaatgctt
    11821 ggacaaggga tcgccgggat cttaccgaaa ctgattggcg gttatttcga taccgaccag
    11881 cgtgcagcgg gcctgggctt tacctacaac gttggcgcat tgggtggcgc actggcccca
    11941 atcatcggcg cgttgatcgc tcaacgtctg gatctgggta ctgcgctggc atcgctctcg
    12001 ttcagcctga catttgtggt gatcctgctg attggtctgg atatgccttc ccgcgttcag
    12061 cgttggctgc gcccggaagc attgcgtact catgacgcta tcgacggcaa accatttagc
    12121 ggtgccgtgc catttggcag cgccaaaaac gatttagtca aaaccaaaag ttaatcctgt
    12181 tgcccggtct atgtaccggg cctttcgcta agggaagatg tatgtcgtta cttgcacaac
    12241 tggatcaaaa aatcgctgct aacggtggcc tgattgtctc ctgccagccg attcctgaca
    12301 gcccgctcga taaacccgaa atcgtcgcag ccatggcatt agcggcagag caggcgggcg
    12361 cggttgccat tcgcatcgaa ggtgtggcaa atctgcaagc cacgcgtgcg gtggtgagcg
    12421 tgccgattat tggaattgtg aaacgcgatc tggaggattc tccggtacgc atcacggcct
    12481 atattgaaga tgtcgatgcg ctggcgcagg cgggcgcgga cattatcgcc attgacggca
    12541 ccgaccgccc gcgtccggtg cctgttgaaa cgctgctggc acgtatacat catcacggtt
    12601 tactggcgat gaccgactgc tcaacgccgg aagacggcct ggcatgccaa aagctgggag
    12661 ccgaaattat tggcactacg ctttctggct ataccacgcc tgaaacgcca gaagagccgg
    12721 atctggcgct ggtgaaaacg ttgagcgaag ccggatgtcg ggtgattgcc gaagggcgtt
    12781 acaacacgcc tgctcaggcg gcagatgcga tgcgccacgg cgcgtgggcg gtgacggtcg
    12841 gttctgccat tacgcgtctt gaacacattt gtcagtggta caacacagcg atgaaaaagg
    12901 cggtgctatg accacactgg cgattgatat cggcggtact aaacttgccg ccgcgctgat
    12961 tggcgctgac gggcagatcc gcgatcgtcg tgaacttcct acgccagcca gccagacacc
    13021 agaagccttg cgtgatgcct tatccgcatt agtctctccg ttgcaagctc atgcgcagcg
    13081 ggttgccatc gcttcaaccg gaattattcg cgacggcagt ttgctggcgc tgaatccgca
    13141 taatctcggt ggattgctac actttccgtt agtcaaaacg ctggaacaac ttaccaattt
    13201 gccgaccatt gccattaacg acgcgcaggc cgcagcatgg gcagagtatc aggcgctgga
    13261 aggcgatgta accgagatgg tgtttatcac cgtttccact ggcgtcggcg gcggtgtagt
    13321 gagcggcggt aaactgctta ccggccctgg cggtctggcg gggcatatcg ggcatacgct
    13381 tgccgatcca cacggcccag tctgcggctg tggacgcaca ggttgcgtgg aagcgattgc
    13441 ttctggtcgc ggtattgcag cggcagcgca aggtgaactc atgggcgcgg atgcgagaac
    13501 tattttcaca cgcgccgggc agggcgacga gcaggcgcag caactgattc accgctccgc
    13561 acatgtgctg gcaaggctga tcgctgatat taaagccaca actgattgcc agtgcgtggt
    13621 ggtcggtggc agcgttggtc tggcagaagg gtatctggcg ttggtggaaa cgtatctggc
    13681 gcaggagcca gcggtatttc atgttgattt actggcggcg cactaccgcc atgatgcagg
    13741 tttacttggg gcagcgctgt tggcccaggg agaaaaatta tgatgatggg tgaagtacgg
    13801 tcattaccgt ctgctgggtt acatcctgcg ttacaggacg cgttaacgct ggcattagct
    13861 gccagaccgc aagaaaaagc gccgggtcgt tacgaattac agggcgacaa tatttttatg
    13921 aatgtcatga cgtttaacac tcaatcgccc gttgagaaaa aagcggagct gcacgagcaa
    13981 tacattgata tccagctgtt attaaacggt gaagaacgga ttctgtttgg catggcaggc
    14041 actgcgcgtc agtgtgaaga gttccaccat gaggatgatt accagctttg cagcaccatt
    14101 gaaaacgagc aagccatcat cctgaaaccg ggaatgttcg ccgtgtttat gctaggagag
    14161 ccgcataagc cgggatgcgt tgttggcgag ccgggtgaga ttaaaaaggt ggtggtgaag
    14221 gttaaggctg atttaatggc ttaaatttat tagatcaggt gtgacctgac ccatctttgg
    14281 cacagagcca aacctgaact gacggaccgc tctgtgccat tacctgacgt tgtattcttt
    14341 tatgttaaca cccttgatac tgtatatacc tgtccgctta attcgcttgc cctttttatt
    14401 cgtaatagtt gctgtaagtg tactgcagcg aatggaatga cgttcatcaa tcaccagccg
    14461 aattgcttct gaagcatctt ccacctctag aaggtcgtga gtcttattta agccgaatgg
    14521 aacaaggtgg aaggcagttt ttcctgatga ttttctggtt atttttactg gatacaacac
    14581 atcaccactt ttatggatta attcaagctg ttcaatgtaa atcacggcaa ctttccttcg
    14641 tttatttgca tgaatataca aaccgaaaat aggagatgca atacaaaagc tgtggaaata
    14701 tcaaacagcc tctcaatttg gcagacagcg tctgccaaat ctttgccgat gggaattctg
    14761 cagatggtgt ctgcggaatt cccaatggtg ttaattcgcc agacactgtc tggcgaattt
    14821 caacatgttg aaaaaagagg taaaaaagcc tcatttggcg taagtttttt gcagagaaac
    14881 cacgcttata ccttagactt aaatcctttg atagtcgctt gattagctgc gcaccatacg
    14941 cagcccttgc ctcgccacct tgttcaaatt cgacaattcg tcggccaatt tcccagtatg
    15001 tcgcggtcat cagcgcgtta acgctacgta ccgtttccgt ccgggcgcta tcgaccaggt
    15061 gaataatacc gtcgtggatt tgctggtagc ctgttgaggt tctctctgag agcgattcca
    15121 ttatctgtcc ctttatatgg cgctgcgttg aataatggaa tcagctatac gccgggcatc
    15181 taccggtaac aatccagatg agcgtattac attggttttc ttcagattat gcgaggcgct
    15241 ttccagaaag aaaggagaat ttcagcgccg actcttttct gaggaggcgg cgctgggaca
    15301 gagcttaaac ttccagccag ttcataatac cgtctgccgc cttacgacct tcggcaatag
    15361 cggttaccac cagatcggaa ccacggacga tatcgccgcc agcaaagatt ttcgggttgc
    15421 tggtctggaa ggcgttgtcg ctgccttccg gggcgatgat gcggccttgt gaatccagct
    15481 cgacgctgtg ttttgccagc cattccatgt tgtgtggacg gaaaccaaac gccatgatca
    15541 ctgcatctgc cggaacgata tgttcggaac ctgcaacgat ctccgcgcgg cgacggcctt
    15601 tggcatccgg ttcgcccatt tcggtacgta ccatttttac gccgctgact ttgccgttac
    15661 cgttcacttc aatccccagt ggctggacgt tgaatttgaa ctctacgcct tcttcccgcg
    15721 cgtttttcac ttcgcggcgg gaacccggca tgttctcttc atcacgacga taggcacagg
    15781 taacgtgctt cgctccctgg cgcacggacg tacgcacgca gtccatcgca gtgtcgccac
    15841 cgccaaggac caccacgcgt ttgccttcca tgctgacgaa cggttcgtcg cgggtttcac
    15901 caaagcccat taactgtttg gtgttggcga taaggaacgg cagcgctgcg tacacgccat
    15961 cggcgtcttc gttttccagc ccgccgcgca ttgactgata agtcccgacg ccaaggaaga
    16021 cggcatcgta atcactcagc agatcgtcca gctgcacgtc gcggcccact tcggtattga
    16081 gtttgaattc aatacccatg ccggtgaaga tttcacggcg acgcgtcatt acctcttttt
    16141 ccagcttgaa ggccggaata ccgaaggtca gcagcccgcc aatttctgga tgacggtcga
    16201 agacaacggc ttttacgccg ttacgcgtca ggacatccgc acacgccaga cctgccgggc
    16261 ctgcgccgat aatcgccact tttttaccgg tttgcttcac gccggacata tccggacgcc
    16321 agcccatttc gaacgcttta tcattgatat aacgctcaat gttgccgatg gtcaccgcgc
    16381 caaactcatc gttcagagtg caggaacctt cgcacagacg gtcttgcggg cagactcgtc
    16441 cgcaaacttc cggcagggtg ttggtctggt gcgacagttc cgccgcttca aaaatacgcc
    16501 cctcgttggc gagcttcagc cagttcggga tgtagttgtg taccggacat ttccactcgc
    16561 agtatgggtt gccgcacgac aggcagcggt cagcctgcgc tttggcctgg ccttcggaaa
    16621 acggctcgta aatttcaaca aactcaattt tgcggatctt cagcggtttc tttggcggat
    16681 caacgcgctg caggtcgata aattgataaa cattctgact cattgttgct accccttact
    16741 gcgcctgcac gcgcaactct gctgcgctac gactacgatg acccagcagt gcttttacat
    16801 cactggactt cggtttaacc agcgcaaatt tagtggcgaa ggttgaccag ttcgccagaa
    16861 tctcttcacc gcgctgagag ccagtatgct gaacatgctc ggtgataaga ccgcgcagat
    16921 gctcttcatg gatcgccaga tcgtcaacgc ttaagacctc gaccagttcc gggttaacgc
    16981 gtttgcggaa atcaccgctt tcatcgagaa cgtaagcgaa gccgccggtc atgcccgcac
    17041 cgaagttaac gccggtttta cccagaatac agacaatacc gcccgtcata tattcacagc
    17101 cgttgtcgcc aatgccttct accacggtga tagcaccgga gttccgcacg ccgaaacgtt
    17161 cacccgcgcg gcctgcggca tacagacgac caccggtcgc gccatacagg caggtgttgc
    17221 cgataatact tgcttcatgg ctgcggaagg cggaaccaac cggaggacga atggcgatta
    17281 agccgcccgc catgccttta ccgacatagt cgttggcatc cccggtcagg tacagttcca
    17341 cgccgcccgc gttccataca ccgaaactct ggcctgcggt gccgttgaag tacgctttga
    17401 taggatcggc tgccagcccc tgatcgccgt gcgtctgggc gatatagcct gaaagcgacg
    17461 cgcccacgga gcgatcggta ttgcggatat cgaaccagaa ggttttgctc tggcgttcat
    17521 cgacaaacgg tttcgcctgt tgcagcaact gcgcgttcag caggccgtta tcaaacggcg
    17581 ggttgttttc ggtgcagtag agtgccttac ctggatgcgg ttcggcggtc tccagcagct
    17641 tcgacagcgc cagtttctgc tgtttggcgg tgaaaccgtc cagctctttc agcaggtcgg
    17701 tgcgaccaat cagatccacc agccgcgtta cccccagctg tgccatcagc tcgcgggttt
    17761 cacgggcgat aaactcaaag taattcgtca ccttgaacgg caggccgtga tagtgattct
    17821 tacgcagttt gtcatcctga gttgccacac ccgttgcgca gttgttcagg tggcaaatac
    17881 gcagatattt acagccgagc gcgaccattg ggccagtgcc gaagccgaag ctttctgcgc
    17941 cgagaatcgc cgctttgatg atatcaacac cggttttcag gccgccatcg acctgcaaac
    18001 ggatcttatg acgcagacca ttggcaacca gcgcctgctg ggtttcaaca agccccagct
    18061 cccacgggca acctgcgtat ttcactgacg acagcgggct tgcgccggtg ccaccgtcat
    18121 agcctgcgat ggtgattaaa tcggcataag cttttgccac gccagtcgcg atggtgccta
    18181 cgcccggttc ggaaaccagc ttcacggaga tcatcgcttt cgggttaacc tgcttgaggt
    18241 cgaaaatgag ctgcgctaag tcctcgatag agtagatatc gtggtgcggc ggcggggaga
    18301 tcagcgtcac tccgggcacc gaatagcgca gtttggcgat gtaaggagtg actttatcac
    18361 ccggcaactg accgccttcg cccggcttcg cgccctgggc gactttaatc tgaatgacgt
    18421 cggcattgac cagatacgcc ggagtaaccc caaagcgacc ggaagccacc tgcttgatgc
    18481 gcgacacttt gttggtgccg tagcgcgccg gatcttcacc gccttcaccg gagtttgagt
    18541 taccgccgat gctgttcatc gcttccgcca gcgcctcgtg ggcttccggg cttaacgcgc
    18601 cgatagacat cgcagcggta tcgaagcgtt taaacagttc gcttgccggt tcaacatcag
    18661 caatgttgac cgcgttttca cccggcgtaa ttgccagcag atcgcgcagc gtggttgccg
    18721 gacgctcatt aaccagcttc gcgtattcct gatagtcgct gtactcgccg ctttgtaccg
    18781 cttgttgcag cgtgcgcacc acgtccgggt tgtaggcgtg gtattcgccg ccgtggacgt
    18841 atttcagcag gccgccctgg ctgatgggct tacgcgccag ccaggcacgt ttcgacagat
    18901 tcagcagatc ctgctggaag tcttcaaagc ttgccccacc aatgcggctg accgccccct
    18961 ggaagcacag gcccactaca tcatcgtgta gaccgaccgc ttcaaacagt ttcgagcagc
    19021 ggtaagaggc gatggtggag atgcccattt tggacatgat tttgtacaag cctttgttga
    19081 tgccgttacg gtagttgagc atcacggtac gataatcttt ggcaatcgca tgggtgtcta
    19141 ccaggcggcc cagcgtttca taggcaaggt atggataaat agccgtcgcg ccgaagccca
    19201 gcaacacggc gaagtggtgc ggatcgcggg cgctggcggt ttcgacgatg atgttggcat
    19261 cgcaacgcag gctttgatcg accagacggg tctggatcgc gccaaccgcc atcggggctg
    19321 gaaccggcag gcgatcttta gcgatattcc ggtcggagag caccagcagc acggtgccgc
    19381 tacgtaccat tttttcggct ttgtcgcaca gctctttgac tgtcgcttcg agcgtggttt
    19441 tagtgacgtc aaaggtgata tccagcgtat ctgcgcggta gtgctcctct ttcatcgtcg
    19501 tgagctgttt gaaatcggag tagagcagaa tcggcgattt aaagcttaaa cggtgcgcct
    19561 ggccctctgc ttcgcaaaag acgttcattt cacgaccgat actggtggcg agcgacataa
    19621 catgcgcttc acgcagcggg tcgattggcg ggttagtcac ctgggcaaac tgctggcgga
    19681 agtagtcgta aataatgcgc ggctgactgg agagcacggc gaatggggta tcatcgccca
    19741 tcgaaccgac cgcttcctga ccgttttcgc ccagtacgcg aattacggag tccagctctt
    19801 ccgcgctgta gttaaactgt ttctggtagc tggcaagcgt gtcgtcgtcc agttcgcggc
    19861 tacccacttc ttcatcgggc agatcttcaa acggtaccag tcggcggacg tttttctcca
    19921 tccactcttt atatggatgg cggcttttca gatcgtcatc ggtttctgcc gagtgcagaa
    19981 tatgcccact gcgggtgtcg ataaccatca gttcgcctgg cccgacgcgg cctttttcga
    20041 ccacttcgtc aggctggtaa tcccagatac cgacttcaga ggcgcaggtg atgagcttat
    20101 ctttggtgat gacgtagcgc gccggacgca gaccgttacg gtcgaggtta caggcggcaa
    20161 aacgaccgtc ggacatcacg atgcccgccg ggccatccca cggctccata tgcatggagt
    20221 taaagtcaaa gaaggcacgc agttccgggt ccatatccgg gttgttctgc caggcgggtg
    20281 gcactaacag acgcatggcg cggatgatat ccatcccgcc tgccagcagg agttccagca
    20341 tgttatccat cgaactggag tcagagccgg tttcgttgac gaacggtgcg gcgtcgtgca
    20401 gatcagggat aagcggcgtc tggaatttat aggtacgcgc acgcgcccat tggcggttac
    20461 cagtgatggt gttgatttca ccgttatgcg ccagatagcg gaacggttgc gccagcggcc
    20521 agcgcggtac ggtgttagtg gagaagcgct ggtggaacag gcaaatggcc gattccagac
    20581 gcaggtccgc aagatccaga taaaagcgcg gcagatccgc cggcatacac agacctttat
    20641 agatgttcac cagattcgat aggctacaga cgtagaagtc tttgtcggct tcgagacgct
    20701 tttcaatgcg gcggcgggcg ataaacagac ggcgctccat atcgcgcgga cgccagcctg
    20761 ccggggcgtt cacaaaaatt tgctcaatgc ggggcagaga ggagagggcg atttcaccca
    20821 gtacgccttc gttagtgggg acatcacgcc agcccacaat cgacaaggtt tcgcgttgca
    20881 gttcttcttc aacgatgcgg cgtgcggcag cggcgagttc aggatcttta ttcaggaata
    20941 gcatcccgac ggcgtagttt ttcgctaaac gccagccgcg ctcctgcgca acgatgcgaa
    21001 aaaagcgatc cggtttttgt aacagcaagc cgcaaccgtc accggtttta ccatcggcga
    21061 gaatcgcgcc acggtgctgc atgcgggcca gtgcgtgtat tgcagtacgc actaccttgt
    21121 ggctaggttc gccttctatg tgggcgatca ggccgaaacc acagttatcc ctctcaaggg
    21181 atttatcgta caacatatcg tgaacctccc ccaggctctg cgggaacccc actaccgaac
    21241 cgctgcgcac gggtacagaa agagcgtggc gacgggggtt gcgtgtcata cgcgcgcctc
    21301 gcattcgccc ccttgttgtc cttttcgcat cggttaatac ggtgaagagg ggatctcaat
    21361 tactgcataa atatgatgag caggctgctc atccagaaag cttccagcgg atttccaact
    21421 tatcgggaaa gctgatacag gtcaaatggc aagcttattg gtacagaaat gtgctttagc
    21481 gcaaaattct ttattaaatg gctgaaatta aaatagattt tatctttaag gctaaaaggg
    21541 aagtcgttag agaaacagtc aattcctgac tgtcataaag gcgtgttaac ccttatcctg
    21601 ccttgaattg acgagaaaac cagcatttta tactgcatta attggtaatg acgtttcatg
    21661 ctactgtttt tgcctaaaat ccataaggag ataaaaaaat ctgacacaag ataagcaaaa
    21721 gtgatttatg tgattatgca tgaaatttcg gtaattttga ttgattatgc agttgataaa
    21781 agcgggccgg gacgattgat ccaggtcatc gccgaattga gctaaaaatg gcaggctttg
    21841 gcctcttttc aaggcgcggt ctatcagatt atgcagttac agaaattagt caatatgttt
    21901 ggtggtgatc tcacccgtcg ttatgggcaa aaggtgcata agctgacgct gcacggcggt
    21961 ttcagttgcc cgaatcgcga cggcaccatc gggcgcggcg gctgcacctt ctgtaatgtt
    22021 gcctcgtttg ccgacgaagc gcagcagcat cgttccattg ccgaacagct ggcgcatcag
    22081 gcgaatttaa ttaatcgcgc gaaacgttac ctcgcctatt tccaggcgta taccagcacc
    22141 tttgccgaag ttcaggtgct gcgttcgatg tatcaacagg cggtgagcca ggccaatatt
    22201 gtcggtttgt gtgtcggcac gcgcccggac tgcgtgccgg atgcggtgct ggatctgctt
    22261 tgcgaatata aagaccaggg ctacgaagtg tggctggagc tggggctgca aaccgtgcac
    22321 gacaaaacgc tacatcgcat taatcgcggt catgatttcg cctgttatca gcgcacgacg
    22381 cagctggcac gccagcgcgg gctgaaggtg tgctcgcatt taattgtcgg tctgccaggc
    22441 gaagggctgg ctgaatgtct gcaaacgctg gagcgggtag tggaaaccgg tgttgacggt
    22501 ataaagctac atccgttgca tattgtgaaa ggcagcatta tggcgaaagc ctgggaagcg
    22561 gggcgtttga acggtattga actggaggat tacacgttca ctgccggaga aatgattcgc
    22621 catacgccac cggaagtgat ctaccaccgc atttccgcca gcgcccgtcg cccgacgctg
    22681 cttgctcctc tgtggtgcga aaatcgatgg acgggaatgg tcgagctgga tcgctatctg
    22741 aatgaacatg gcgtacaggg atcggcgctg ggacgtccgt ggctacctcc aacggagtag
    22801 gtcgttgagg ggaattccgc atttctcaca caatttataa cgtaactgtc agaattgggt
    22861 attattgggg caggttgtcg tgaaggaatt ccctaatgaa gcaaattcgt ctgctggcgc
    22921 agtattatgt tgacctgatg atgaagttag gtctggtgcg cttctcaatg ttgctggcgc
    22981 tggccctcgt cgttcttgcc attgtggtac aaatggcggt aaccatggtg ctgcatggtc
    23041 aggtcgaaag cattgatgtt attcgttcta tcttctttgg tttgttgatt acgccgtggg
    23101 cggtctactt tctatcggtg gtcgtcgagc aactggagga gtcacgacaa cgtctgtcac
    23161 ggctggtgca aaaactggag gagatgcgcg agcgcgattt gagcctcaac gttcagttaa
    23221 aagataatat tgcccagcta aatcaggaaa tcgccgttcg tgaaaaagcg gaagcagaac
    23281 tgcaggaaac cttcggccaa ctgaaaattg aaatcaaaga gcgcgaagag acacaaattc
    23341 agctcgagca gcaatcctca ttcttacgtt ccttccttga tgcttcaccc gacctggttt
    23401 tttatcgtaa cgaagataaa gagttttccg gctgtaaccg cgcgatggag ctgctgaccg
    23461 gaaaaagcga aaaacaactg gttcacctga aacctgttga tgtttactca ccggaagccg
    23521 ccgcaaaagt cattgaaacc gatgaaaaag tgttccgtca taatgtgtca ctgacctatg
    23581 aacagtggct ggattacccg gacgggcgca aagcctgctt tgaaatccgt aaagtgccgt
    23641 actacgaccg cgtgggtaaa cgtcacggtt tgatgggctt tggtcgagac attaccgagc
    23701 gtaagcggta tcaggatgcg cttgaacggg ccagccgcga caaaacgacg tttatctcca
    23761 ccatcagtca cgaattgcgt acaccgctga acggtatcgt cggtctgagc cgcattctgc
    23821 tggataccga actcaccgcc gagcaggaaa aatatctcaa gaccatccat gtttcggccg
    23881 tcacgctggg gaatatcttt aacgatatta tcgacatgga taagatggaa cggcgcaagg
    23941 tccagcttga taatcaaccg gttgatttca ccagcttcct tgccgatctg gaaaatctct
    24001 ccgcattgca ggcgcaacaa aaaggattgc gctttaacct ggagccgacg ctgccattac
    24061 cgcatcaggt cattaccgac gggacgcgtt tacggcagat cctgtggaac ctcatcagta
    24121 acgccgtcaa attcacccag caaggccagg ttaccgtgcg cgtgcgctac gatgaaggcg
    24181 atatgctgca ttttgaagtg gaagactctg gtatcggcat tccgcaggat gagctggata
    24241 aaattttcgc catgtattac caggtgaaag acagtcatgg cggtaaacct gccaccggca
    24301 ccggtattgg tctggccgtt tctcgtcgtc tggcgaaaaa tatgggcggc gatattacgg
    24361 ttaccagcga acagggcaaa ggttcaacct ttacgttgac gatccacgca ccgtcggtag
    24421 cagaagaggt cgatgatgcg tttgatgaag acgatatgcc tttaccggcg ctgaatgtgc
    24481 tgctggtgga agacattgaa ctgaacgtga ttgttgcgcg ttctgtgctg gaaaaattag
    24541 gtaacagcgt tgatgtcgcc atgaccggca aggcggcgct agagatgttt aaaccgggcg
    24601 aatacgacct ggtgttactg gatattcagt tgccagatat gaccgggctg gatatctctc
    24661 gtgaactgac gaagcgttat ccgcgagagg atttaccgcc gctggtggcc ttaaccgcta
    24721 acgtgctgaa agacaaacaa gagtacctca atgctggaat ggatgatgtg ctgagtaagc
    24781 cgctttctgt tccggcgcta accgcgatga tcaagaaatt ctgggatacc caggatgatg
    24841 aggagagtac ggtgacgaca gaagagaaca gtaaatcaga agcattgctc gatattccca
    24901 tgctggaaca gtatctcgaa cttgtaggac cgaagctgat caccgacggg ttagcggtgt
    24961 ttgagaagat gatgccgggc tatgtcagcg tgctggagtc gaatctgacg gcgcaggata
    25021 aaaaaggcat tgttgaggaa ggacataaaa ttaaaggtgc ggcggggtca gtggggttac
    25081 gccatctgca acagctgggt cagcaaattc agtctcctga ccttcctgcc tgggaagata
    25141 acgtcggtga atggattgaa gagatgaaag aagagtggcg tcacgacgta gaagtgctga
    25201 aagcgtgggt ggcaaaagcc actaaaaaat gaccccggct agaccggggt gcgcgaatac
    25261 tgcgccaaca ccagggaaat cttggctgcg ccgtaaatta ttatgatgag ttacaagggc
    25321 acagcactgt ttttcaggcc gcacgcagtt aagatagcaa aacttaaatg atttgttaca
    25381 tgaatcagtt aaatgtgtga tgcgtaccat ttttattaaa aaaattaatg tgtttcagtc
    25441 aattgcgaaa ggatgatcac aatgaagaaa attggcgtaa ttctgagcgg atgcggcgtc
    25501 tatgacggtt ctgaaattca tgaagcggtg ttgacgttgt tagctatttc acgcagcggt
    25561 gcgcaggcgg tctgctttgc accggataag cagcaggttg atgttatcaa ccatttaact
    25621 ggcgaagcga tgacggaaac gcgcaacgtg ctgattgaag cggcacgaat aacgcgcggt
    25681 gaaatccgtc ctctggccca ggccgatgcc gctgaactgg atgcgttgat tgtgccgggg
    25741 gggtttggcg cggcgaagaa tttaagcaat tttgccactc ttggtagcga atgcaccgtt
    25801 gaccgtgaat taaaggcgct ggcacaagcg atgcatcagg ccggaaaacc gcttggtttt
    25861 atgtgtattg ccccggcgat gctgccgaaa attttcgatt tcccgctgcg tttgaccatc
    25921 ggtactgata tcgataccgc agaagtgctg gaagagatgg gcgcggagca tgtgccctgt
    25981 cctgtcgatg atatcgtggt tgatgaagac aataagattg tcaccacccc agcatatatg
    26041 ctggcgcaga acattgcaga agcggcgagc ggcattgata agctggtttc ccgcgtgctg
    26101 gttctggctg aatgagtaaa agccgcttaa cggtgtttag tttcgttcgc cgttttcttt
    26161 tgctgttaat ggttgtcctc gccgttttct ggggcggggg catcgcgttg tttagtgttg
    26221 cgcctgttcc cttctcagcg gtaatggtcg agcgacaggt cagcgcctgg ctgcatggca
    26281 attttcgtta cgtggcacat tctgactggg tcagtatgga tcaaatctcg ccgtggatgg
    26341 gactggcggt gattgccgca gaagatcaga aattttctga gcactggggc tttgatgtcg
    26401 cttccattga gaaagccctg gcgcacaacg agcgcaatga aaaccgtatt cgcggtgctt
    26461 caacgatttc tcagcagacg gccaaaaatc tctttttatg ggatgggcgt agctgggtac
    26521 gaaaagggct ggaagctgga ttaacgctgg ggatagaaac agtctggagc aaaaagcgta
    26581 tcctgacggt ttacctgaat atcgccgaat ttggcgacgg tgtgtttggc gtcgaagctg
    26641 cggcacaacg ttatttccac aaacccgcga gcaaacttac ccggtcggaa gctgcattac
    26701 tggcagctgt attacctaat ccacttcgtt tcaaagtctc ctcgccatcg ggctacgtgc
    26761 gtagccgtca ggcgtggatt ttacggcaga tgtatcagtt aggcggtgag ccgtttatgc
    26821 aacaacacca gctggattaa gcttcgcgcg aagtgagggc cagcgttttg ctctcacgag
    26881 gttgcagatc tttctgcagg gcaatagcca gcgccggttg ggcgataaat cgtttccata
    26941 accgctcttg cttacgcaga caacaccact tcgaccaggt cgccagtgga atcaatgtgc
    27001 tttcaccaat gttgtcgcag acgaccggga taacctctga ctcactgatg cggtgacaga
    27061 ggatattctg cggcttcagc gacatcgtca cgatacggtt atcctgcaaa taacgcttca
    27121 gctgtttcag tagctggcgc agttgtgcaa tgtcttcttc gtaacggcat tgttcagcaa
    27181 attcggtcag cgtaatggag agtttgccgt caaaatctgc aatcacatcg tagacataac
    27241 cggttccgca atccgtctca acggtaccgt gatagcgcgg tattccactc cagtctttta
    27301 accggcgacc aagatgcgcg tagtacttta actcacggcg gatctcttta tcgccaccat
    27361 cgccacgatg gtagacaatc ttgatacagc gttgggcatc ttccggatgc gcataacatt
    27421 tccgatgtct tccggtgccc aggggacttt gttcagataa gcgaatcatg ccatctcctg
    27481 gaaatatttt ctgacgatct ccagtatggt cgattaactt aaacaaaacc taaacaatgc
    27541 ctcttaaggt tttcttaagg ttcttctgaa agtgaaaagc gcacaaaatg tacaaatgac
    27601 gttgattgag aacgatttca gtacgtgatt ttcttattgt cggggggagt ttgaagggag
    27661 ttgtatgtca aagtgatgaa gattaatctt catcaaaacc agagttaaag agggcgataa
    27721 cggcggccag tgcttcctct tcctgtgggc cggtcgcttc aacttcaatc tgccgtcctt
    27781 tggcagaatc caacatcagc agcgcaataa cgctgttggc ttcggcctcg gtgccttcgt
    27841 cattacgtaa cagcacttca gcgtcaaaac cctgcattaa ttcaaacagc ttcattgcag
    27901 gccgggcatg catgcccagc ttgtttgtga tttcaacagt ttgcttgacg gtcatggttt
    27961 acgtttttcc agcgtacgat ggcgtgactg gacgttttta ccgcgcgagc ggaagtagtc
    28021 tgccagttgc tctgcaatat acaccgaacg gtgcttcccg ccggtacaac caatggcgac
    28081 cgtcaagtag ctacggttgt tggtttccag cataggtaac catagctcaa gatagcttcg
    28141 cgtctggtag ataaaattgt gtacttctgt gtggcggtcg aggaacgcgg cgacaggttt
    28201 atcaagacct gtcattggac gcagtttcgg atcccagtgc gggttcggca agaagcgcac
    28261 gtcaaagacg taatctgcat cgatagggat accgtgtttg aagccgaaag actcaaagac
    28321 catggtcagt tcgcgttcac gtttacccag cagacgggta cgcagcattt cagccagctc
    28381 gtgaacggac atttctgagg tatcgacaat cagatccgct cgcgaacgca gaggctccag
    28441 caaatcgctt tctttgtcga tagcactttc cagcgacagg tttttgctgg aaagcggatg
    28501 cagtcggcgc gtgtcactgt aacgacgaat taaggtatta cggtcggcat ccaggaacag
    28561 gagttgcggt gagaaagcgt caggcaggtt actcatcgcc tgttcgaata tttctggtga
    28621 ctccggcata ttacgaacat caatgctgac ggcggcagaa atctcgcgat cggccagggt
    28681 tcgagccaga tcgggtaaca acactacggg aaggttatcc acgcagtaaa aacccatatc
    28741 ttccagcgca cgcagggcga cagatttacc tgaacctgaa cgtccgctga cgatcatcag
    28801 taccatgtac tgtttctcct cacaacgtct aaaagagaca ttaccgaata actacgcttc
    28861 atccggagta ccttcggtat ccgtaatgat ttgatacagc tcttcatcgc tctgggctgc
    28921 gcgtaaacgg cggcagatgg ttttgtccgc cagacgtttc gccaccagcg acagagtatg
    28981 caggtgcgtt ttagtttggt ctgccgacac cagcagggca aaaagtaagt ccaccggttg
    29041 gttgtcgatg gcatcgaaag ctataggcgt ttcgagctga acgaaaacgc caacggcgcg
    29101 cagagtatct tcttccagtt tgccatgcgg aatggcaata ccattgccga taccggtact
    29161 gcccattttt tcccgcgtca ggatagcttc aaaaaccacc tgaggcggca ggctaagttg
    29221 tttcgccgcc agctcgctga tgatttccag ggcgcgtttt ttgctctgac agtggacgcg
    29281 gcttcgcgta cattccctgt taagaacact gctaagctgt agagttgtat cattatttgt
    29341 cataatttca cctaagaacc tgcccactca aacagtggaa caggctcgga gcattaaccg
    29401 tacaaatggc ccgttgtgcc gcacaacagg ccgccatgca catgctaatt gcccggacaa
    29461 ttagtgttgt ttcagtttat ctttatgttt ggtcaactgg cgtgccagct tatcaattaa
    29521 gccatcaatg gcagcgtaca tgtcctgacc ttccgcgctg gcatgaattt caccaccgtt
    29581 tacatgcagt gttgcatctg aggtgtgggt gactttctcc actttcagaa caacatagac
    29641 ctggttgatt cggtcaaaat attgctcaag tttggcaaat ttggctgtaa caaattcgcg
    29701 cagtgcctcg gtgatctcga cgttatttcc ggtaatgttg agctgcatag tgtcttcctt
    29761 atcggttggg tcaaacgagc tgtttacgct ggtttgacgg cggaatggat aaagactctc
    29821 ggtacttcgc aacagtgcgg cgtgccacca tgataccttg ttccgacagc aaagaggtta
    29881 acttgctgtc gctcaacggt ttcgctgggt tttccgccgc gattaatttc ttcaccagcg
    29941 cacgaatcgc cgtggaggaa gcttcgccgc cgccctcggt attgatgtga ctggaaaaga
    30001 aatacttcag ttcaaaaatg cctcgtggac tatgcaggta tttttgcgtg gtcacgcgag
    30061 atatcgtcga ttcatgcatt tcgacagcct gggcgatatc ggccagtacc atcggtttca
    30121 tatattcttc accttgctca aagaaggctt gctgctgttc aacgatacag cgactcacgc
    30181 gcagtagcgt atcgttacgg ctttccagac tcttgatcaa ccatttggca tcctgcagat
    30241 tgctgcggat aaactggctg tcaccatcgt tgcgcgcgtt attgcacatc gaggcgtagt
    30301 gctggttgat ttgcagacgc ggaatgctgt cactgttgag ttctaccgtc cagtgaccgt
    30361 tatgcttacg caccagcaca tctggaatga catactcagg ttcgccagtc tggatcgact
    30421 gcccggggcg cggatcgagc gactggatca gattgacggc ttctttcagc acatcttctt
    30481 tcagacgcgt gacgcgcatt aaagtgcgga agtcgtgatt ggctaacaga tcgagatgat
    30541 cgctaatgat cagtctggcc tcttccagcc acggcgtggt tttatcgaat tgggagagtt
    30601 ggattagcag gcagtcacgc agatcttttg ccgctacacc gaccggatca aaccgttgga
    30661 tccgcttaag gacggcttca acctcgtcga tgtcgatctc ttcatcgccc atactttcga
    30721 gaatatcttc cagcgggaca gtcagataac cgatgtcatc aacggcatcg acgatagagg
    30781 tagcaatagc gcggtcagtg tcggaaaacg gtgtcagctc gacctgccac atcaggtaat
    30841 cctgcaaggt ctgcgtcgtt tcgccctgat agaccggcag ctcatcgtca atgtagtcac
    30901 cgctggtgcc ggatggtgta ccagcggtgt aaatggtgtc ccaactggca tcgagcggca
    30961 gctcttccgg catctctttt tgttcgagcg cgtcggcggt gtccagcgtt tcactgtctt
    31021 gcgtttcgcg ggtgtcgatt tcttcatgag tgtcgatttg ctcaagcagc ggattactct
    31081 ccagcgcctg ctgtagctcc tgctgaagtt ccagcgtcga caactgcaac agacgaattg
    31141 cctgttggag ctgtggcgtc atcgccagtt gttggctaag cctgagttgc aaaccttgct
    31201 tcatgttcag aatcgtactc tcctgctaaa acgtcgcaaa cttctaccct atcagagtct
    31261 gaagtcttcc ccaaggtata cacgcttaac gtgttcgtct tgtaagattt ctgtaggcgt
    31321 gccgtgggcg atcaaatgcc cctgactgac gatataagcg cgttcacaaa ccgccagtgt
    31381 ttcacgcacg ttgtggtcag tgatcagcac gcccaggccg ctgtcgcgca ggtgctcaat
    31441 gatgcgttta atgtcgataa ccgagatcgg gtcaaccccg gcaaacggtt cgtcgagcag
    31501 aataaatttc ggattcgcag ccagtgcgcg ggcaatttct acacggcgac gttcaccccc
    31561 ggagagtgac tgccccatgc tgtcacgcag gtgctcaatg tgaaactctt ccatcagctc
    31621 gttcgcgcgg tcttcacgtt gttcagcaga caagtcgtca cgaatttgca gtaccgccat
    31681 caggttatcg taaacgctga ggcgacggaa aatggaggct tcctgtggca gatagccgat
    31741 accgcggcgc gcgcgtgcat gcagaggcag cagactgata tcgtcatcat caataatgat
    31801 gttgcccgca tcgcgcggca caatgcctac aaccatgtag aaagtggtgg tcttaccggc
    31861 accgtttggc cccagcagac cgacaatttc cccggagttg acggtcaggc tgacgtcttc
    31921 taccacgcga cggcctttat aggcttttgc aaggttcttt gcagttaatg ttgccataac
    31981 gaattaatta cccttcttct gtgccggggt ctggcctttg ttgtttttgt cctgcagctg
    32041 cgacggcacc agaacggtgg ttacgcgctt gcctttgtcg ctgaaagcct gcattttctg
    32101 ctcttttacc aggtaagtga tcttatcgcc cttaatgttg ctatcgacct gctgcagata
    32161 agcattaccc gtcagaacga caaaatcttt tgccagttcg tagtgcatct gggaagcgtg
    32221 accttcaacg ggtttaccgt tgtcctgcat ctggtagaac gttgccggtt taccgtagcc
    32281 gtcaatcact tctttacctt gttcgccgcc cggacgggta acgaccactt tgtcggcatt
    32341 aattttgatg gtgccctggg tgacgatgac attaccggta aaggtaacca cgttgccttg
    32401 catatcaaga gattgctggt ccgattcaat gtggatcggc tgatcagtgt ctccggttac
    32461 ggcaaatgcc ggaatgctgg cggccagaag tgagctggca agcacaagat taaggctgag
    32521 tttgtttgtt ttgaatttca taggatgttc taaccttttc aatcagctcg gcgttcttgc
    32581 tgcgtaagtt gccgcgcatt ttcagaccgc tggagttaaa tgttgttccg tataacgtga
    32641 cgaggtcttc agaggtaaca tcctgcgtca ccagattgat ctgcgcgtta tccgtcgtga
    32701 ttctgcgaag ttgagagttt ggcacaagtg cgttgacttc aacgtgtcca tataaataga
    32761 gcatccggtc attggtcagc ttggctttat ctgcttttac ggaccatgtc gggattttat
    32821 ccttatcaaa cgtggtaagt accggctgcg taaaccacga aacggcctga tcggaataat
    32881 attcaacgtg ttgagcaatc aatcgatagc ttagtgcccc ttctgggtta tagacgagcg
    32941 tgtccgtatg ctcgctttta taggtgggat cattgttgtt gacgaccacc tgggcggtat
    33001 cgtctttttc ggccatatca atgccgatca tcaccagaac cgccagtgat agcacaatga
    33061 taacccaacg tctggcttta ctcatatcga ttgccctttg gcttcatcca gtttgccctg
    33121 cgccaggagt aataagtcgc aaacttcgcg cactgcgcca cgaccgccag caatgcgcgt
    33181 cacgtaatcg gcgcgcggga tcaacagtgg atgcgcatcg gccacggcga cgcttaaacc
    33241 cactttttcc attaccggcc agtcgatgag atcatcgccg acataagcca cattttccgg
    33301 ggcaatcgcc agtttttcca gcagatcgct aaaggcgatc agtttgtttg actgcccctg
    33361 atacaagtga gtgatcccca atgtggcaca acgatcttct accagtttag cctttcgccc
    33421 ggtaatgata gcgacttcaa tatcagaggt gagcgcacaa cgaatgccat aaccgtcacg
    33481 aacattgaac gctttcagct cttcgccatt attgcccata taaatcaggc catctgacag
    33541 tacgccatcg acatcgagga tcagcagacg aatgttctct gcttttgcta taacgtcggc
    33601 gctgacaggg ccgtaacagg tcgcaagcga cgcacctgct ttgctcattg ttgtttatcc
    33661 ttgaatcttt acactacgcc tgcacgcagt aaatcatgca tatgtaacac tccgagtaaa
    33721 tggtcgccat cggcaaccat cacggaggtg atatggcggg actgcattaa gttcagtgcc
    33781 tcaacggcca gaatgccagg gcgcacacgt attccccccg gcgtcatcac atcggcaata
    33841 cttaactgac gaacatccac gcccatatcg aagacacggc gtaaatcacc gtcggtaaag
    33901 atgccttcaa tcatcatatt gtcatcgcaa atgacagtca taccaagatt tttgcgggta
    33961 acttccagca acgcgtcacg cagactggcc gttttcttaa catgcgggat ctcatcgccc
    34021 gtatgcataa tatcgtttac gcgcagcaga agtttacgac ccagtgcgcc gcctgggtgt
    34081 gagagcgcaa aatcttcagc agtaaagccg cgtgctttta acagcgcgac agcgagggca
    34141 tcgcccataa ccagcgtggc ggtggtgctg ctggtcggtg ccagccctaa cggacaggct
    34201 tctttcgcta ctttaacaca cagatgcaca tctgcggcgc gcgccatgct gctctccggg
    34261 cgaccggtga tgcagattaa cggtacgtga agacgcttaa gcactggaat taaggccgtg
    34321 atttcgctgg attcacctga gttagagata gcaatcacca catcctgtgg ggtgaccatg
    34381 cctaaatcac catgcgcggc ttcaccagga tggacgaaaa atgaaggtgt accggtgctg
    34441 gcaaacgttg cggccatttt gcgcccgatg tgccccgatt tccccatccc catgacgaca
    34501 actttccctt tacaccagaa catcttttca caggcaagcg tgaaattctg attgatgtat
    34561 tgatcaagct ccgccaggca ttcacgttca atcgccagga cttctttacc tgcttgctga
    34621 aagtcaaaac ccggttgtaa ctctacgtgc gacataatgc gtttccagtt attcaacgag
    34681 tattggcgat aaccagtaca acatcgccag ccatacgata aatccgccag ttaataatac
    34741 ccctacacca cggcccggtt gcggggagcg ccgccagcac agcaacgcaa aaataatgct
    34801 caccagcaac atcacgctgt agtcacgact gtacgccaat ggctcaatct ctcctggcgt
    34861 tatcagcgcg ggtaaaccca acacgatgac aatattaaaa atgtttgcgc caatgatatt
    34921 tccgacagca atgtcgtttt cacctttgcg aacccccgct attgcggttg ccagttccgg
    34981 caggctggtt ccgatagcaa ttgccgtcag acccatcgtc aactcgctga tggcaaagta
    35041 attcgccagc accgtggcgt tatcaaccac catccgcgtg gccactggca tgatgataag
    35101 cgcaatgccg agccataaaa acgcgacggg caatccgccg tcacgcggca gctctgcaag
    35161 ctgctctctg gtcaggctgt cagtcccctg acgttcagcc tgacgtgcaa gtttaacaat
    35221 gaacagcagc catagcacag ccagaaagag gagaaagata ccatcgctgc gactaagttg
    35281 tccgtcatag agtacggaac cggccactac gctgaccaac aacattaagg gtaattcacg
    35341 acgcagaaca tcggaatgga cggtaaaagg acgaaccagc gcagccagac cgaggatcag
    35401 caaaatattg ataatgtttg agccgagggc tgtaccaacg gctaaatcgc gttgttcgtg
    35461 cagagacgcg gcaagcgaga cgatgatttc tggtaacgat gtaccaatac tgaccaccgt
    35521 catgccgatg atcagcggcg ggatgccaaa ggttcggcaa agaatagacg cggcaaaaac
    35581 caggcggtcg gcactgtaaa cgaccaaaag taaaccaaca attaacagtg ccgtagctaa
    35641 aagcatctaa cgtcctttct tcaggtatac tcgtcggtcc gctgaagatt ttcagaaagc
    35701 cgtaacggat tcttaatttt gactttatgc ggctaaaaag taaaacaaat gccagctttc
    35761 gctaaccacg gcgggtaata ttctgtaaat atgttgggtt caaggttaaa ttgagcgcca
    35821 tgcttagaaa atcaacgcaa gacgaagggt gaattatgga gcagtctgtg gcgaatttag
    35881 tcgatatgcg cgatgtcagt tttacgcgtg gcaatcgctg catcttcgat aatatttccc
    35941 tgaccgtgcc gcgagggaag atcacggcga tcatggggcc atcgggcatc ggtaaaacga
    36001 cgctactccg tctgattggc gggcaaatcg caccagatca tggtgagatc cttttcgatg
    36061 gtgagaatat tccggcgatg tctcgttcgc gcctgtatac agtgcgcaaa cggatgagca
    36121 tgttatttca gtccggggcg ttgttcactg atatgaacgt atttgacaac gtcgcctatc
    36181 cactgcgcga acatacccaa ctacccgcgc cattgttgca tagtacggtg atgatgaagc
    36241 tggaggccgt ggggctgcgt ggagcggcta aactaatgcc ttctgaactt tccggtggga
    36301 tggcgcggcg tgcagcgctg gcgcgtgcga ttgcgctgga gccggatctc atcatgtttg
    36361 atgaaccttt tgttgggcaa gatcccatca ccatgggcgt actggtgaag ctgatttctg
    36421 agctgaacag cgcgctgggc gtgacttgtg tggtggtttc tcacgatgtg ccggaagtgt
    36481 taagtattgc ggatcacgcc tggatcctgg cggacaaaaa aattgtcgct catggcagtg
    36541 cccaggcgtt gcaggcgaat cctgatccgc gcgtacgtca gtttctggac gggatagctg
    36601 acgggcctgt tccgttccgc tatcctgccg gcgattatca cgctgatctt ttaccaggga
    36661 gttaagccac tcatgctgtt aaatgcgctg gcgtcgctcg gacataaagg gattaaaacc
    36721 ctgagaacgt tcgggcgggc cgggttaatg ttattcaatg cgctggtcgg caaaccggaa
    36781 tttcgcaaac atgcgccgct gctggtgcgc cagctctata atgtcggcgt cctgtcgatg
    36841 ctgattattg tggtttctgg cgtgttcatc ggaatggtgt tggggctgca aggttatctg
    36901 gttctgacca cttatagtgc ggaaaccagt ctgggtatgc tggtggcgtt atcgctactg
    36961 cgtgaactgg ggccggtggt tgccgcgttg ttgtttgccg ggcgtgctgg ttcggcgcta
    37021 accgcagaaa tcggcctgat gcgcgctaca gagcaactct ccagtatgga gatgatggcg
    37081 gtggatccgc tgcgtcgggt tatttctccc cgtttctggg ctggggttat ttcattacca
    37141 ctgttgacgg ttattttcgt cgccgtgggg atctggggcg gatctctggt cggcgtcagt
    37201 tggaaaggca ttgatagcgg gttcttctgg tcggcaatgc aaaatgccgt cgactggcgt
    37261 atggatctgg ccaactgtct gattaagagc gtggtgttcg ccatcacggt gacgtggatt
    37321 tcgttgttta acggctacga caccatcccg acgtctgccg ggattagccg ggcaaccact
    37381 cgcaccgttg tccactcgtc tctggctgtt ctggggctgg attttgtgct gaccgcattg
    37441 atgtttggga attgagttca tgcaaacgaa aaaaaatgaa atttgggtgg gtatcttttt
    37501 attagcagca ctgctggcgg cgctgtttgt ttgcctgaag gcggcgaacg tgacgtccat
    37561 acgtactgaa ccgacctaca cgctttatgc gacgttcgat aacattggcg gcctgaaagc
    37621 gcgctctccg gtcagtattg gtggcgttgt tgtgggtcgg gtggcggata ttacgctgga
    37681 cccgaaaacc tatctgccgc gcgtaacgct ggaaattgaa caacgttata accacattcc
    37741 agataccagt tcgctgagca ttcgtacttc cggcctgctg ggggaacaat atctggcatt
    37801 aaacgtcggt tttgaagacc cggaactggg gactgctatc ctgaaggatg gcgatacaat
    37861 tcaggacact aagtctgcga tggtgctgga agatctcatt ggtcagttcc tttacggtag
    37921 taaaggcgat gacaataaga atagtggcga tgcgccagct gctgcgccag gtaataatga
    37981 aaccactgaa cctgtgggta caacgaaata atttcaggag aaccgacgca tgtttaaacg
    38041 tttaatgatg gtcgctttgc tggtgattgc acctctgagt gcggcaaccg cggcagacca
    38101 gaccaatcct tataagctga tggacgaggc ggcgcagaaa acgttcgatc gcctgaagaa
    38161 tgagcaaccg caaattcggg ccaacccgga ttatctgcgt accattgttg atcaggaact
    38221 gctgccatac gtacaggtga aatacgccgg tgcgctggtg ctgggccagt attacaagag
    38281 tgcgacccct gctcaacgtg aagcctactt tgccgctttc cgtgagtacc tgaagcaggc
    38341 ttacggtcag gcgctggcga tgtatcacgg tcaaacctat cagattgcgc cagaacagcc
    38401 gctgggcgat aaaaccattg tgcctattcg cgttaccatt attgacccga atggccgtcc
    38461 gccggtgcgt ctggacttcc agtggcgtaa aaactcccag acgggaaact ggcaggctta
    38521 cgacatgatt gctgaaggcg tcagtatgat caccaccaaa caaaacgagt ggggaacgct
    38581 gctgcgtacc aaaggtatcg acggcctgac tgcgcaactg aaatcgattt ctcaacagaa
    38641 aatcactctg gaagagaaaa aataatgagc gagtcactga gctggatgca gacgggtgac
    38701 acgctggcgt tatccggaga gctggatcag gacgttctgc taccgctttg ggaaatgcgt
    38761 gaggaagcgg tgaaggggat tacctgcatc gatcttagcc gtgtgtcccg cgtggatacg
    38821 ggtggactgg cactgctgct ccatcttatc gatctggcga aaaagcaggg caacaacgtg
    38881 acgcttcagg gggtaaacga caaagtgtat accctcgcga aattgtataa tttgcctgct
    38941 gatgttctgc ctcgttaaat tttttcaggc cggatatggc gttaaccgcc tgtccggcaa
    39001 acctgcctgc aagtcgagcc ttgtctgatt tatcagcgag gctttttgct tatttatgcc
    39061 agcgcaactt tgctctaaga tgtttcgctg gtttattaac tgatgattga agatcccatg
    39121 gaaaataatg aaattcagag cgtgttgatg aacgctctct ccctccagga agtccacgtt
    39181 tccggcgatg gcagccactt tcaggttatt gccgtgggtg agttgtttga cggcatgagt
    39241 cgggttaaaa aacagcagac ggtctatggc ccgctgatgg aatatattgc ggataaccgc
    39301 attcatgctg tgtcgatcaa agcgtatacc cctgcggagt gggcgcgcga tcgcaaactg
    39361 aacggctttt gagctatggg cgattcgccg gtagcggata tgaattgtta actgagaaca
    39421 aactaaatgg ataaatttcg tgttcagggg ccaacgaagc tccagggcga agtcacaatt
    39481 tccggcgcta aaaatgctgc tctgcctatc ctttttgccg ctctactggc ggaagaaccg
    39541 gtagagatcc agaacgtccc aaaactgaaa gacgtcgata catcaatgaa gctgctaagc
    39601 cagctgggtg cgaaagtaga acgtaatggt tctgtgcata ttgatgcccg cgacgttaat
    39661 gtattctgcg caccttacga tctggttaaa accatgcgtg cttctatctg ggcactgggg
    39721 ccgctggtag cgcgctttgg tcaggggcaa gtttcactgc ctggcggttg tacgatcggt
    39781 gcgcgtccgg ttgatctaca catttctggc ctcgaacaat taggcgcgac catcaaactg
    39841 gaagaaggtt acgttaaagc ttccgtcgat ggtcgtctga aaggcgcaca tatcgtgatg
    39901 gataaagtca gcgttggcgc aacggtgacc atcatgtgtg ctgcaaccct ggcggaaggt
    39961 accacgatta ttgaaaatgc agcgcgtgaa ccggaaatcg tcgataccgc gaacttcctg
    40021 attacgctgg gtgcgaaaat tagcggtcag ggcaccgatc gtatcgtcat cgaaggtgtg
    40081 gaacgtttag gcggcggtgt ctatcgcgtg ctaccggatc gtatcgaaac cggtactttc
    40141 ctggtggcgg cggcgatttc tcgcggcaaa attatctgcc gtaacgcgca gccagatact
    40201 ctcgacgccg tgctggcgaa actgcgtgac gctggagcgg acatcgaagt cggcgaagac
    40261 tggattagcc tggatatgca tggcaaacgt ccgaaggctg ttaacgtacg taccgcgccg
    40321 catccggcat tcccgaccga tatgcaggcc cagttcacgc tgttgaacct ggtggcagaa
    40381 gggaccggat tcatcaccga aacggtcttt gaaaaccgct ttatgcatgt gccggagctt
    40441 agccgtatgg gcgcgcatgc cgaaatcgaa agcaataccg ttatttgtca cggtgttgaa
    40501 aaactttctg gcgcacaggt tatggcaacc gatctgcgtg catcagcaag cctggtgctg
    40561 gctggctgta ttgcggaagg gacgacggtg gttgatcgta tttatcacat cgatcgtggc
    40621 tacgaacgca ttgaagacaa actgcgcgct ttaggtgcaa atattgagcg tgtgaaaggc
    40681 gagtaatcgt ctgagagctg gcagccccgt tcgcggggct accgccagac cactcatttt
    40741 ttcggtttag tgtagctacg catcaactgc gttctgtcga taaactcatg ggtctgcgga
    40801 tcatggtagc gtgatggcca gataacccag gggtcagttc ccagggcttt cgcaataatc
    40861 atctctcctt tcggccatgg gcgcgataat gcgtttgcca gcgttgagga actcaaacca
    40921 tttctgcgag actccgccgc cattgacgtt cccttcttgc gcaaacccgc aatgatgtca
    40981 gcgggatgcc agtcaatgaa attactttcc atctttcctc actccttaag ttagtcgctt
    41041 tcattcagaa cacttcttct gtcgggcatc actatactcc tgatggccta ttgctctaaa
    41101 aagttcctgt aaagagtgtg aatattcaga atatataaag attcatcgcc cttttttttc
    41161 aggtgttata gcgctgaatt agtgtttt
//
LOCUS       DAZPAC010000013        39258 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZPAC010000013 DAZPAC010000000
VERSION     DAZPAC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39258
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.013"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHUPM_000566"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHUPM_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR0868447.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHUPM_000567"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHUPM_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR0868448.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHUPM_000568"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHUPM_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR0868449.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="ACHUPM_000569"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="ACHUPM_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR0868450.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="ACHUPM_000570"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="ACHUPM_000570"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR0868451.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHUPM_000571"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHUPM_000571"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR0868452.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="ACHUPM_000572"
     CDS             complement(6574..7338)
                     /locus_tag="ACHUPM_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0868453.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHUPM_000573"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHUPM_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR0868454.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="ACHUPM_000574"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="ACHUPM_000574"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR0868455.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="ACHUPM_000575"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="ACHUPM_000575"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR0868456.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="ACHUPM_000576"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="ACHUPM_000576"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR0868457.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="ACHUPM_000577"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="ACHUPM_000577"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR0868458.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="ACHUPM_000578"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="ACHUPM_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR0868459.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="ACHUPM_000579"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="ACHUPM_000579"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR0868460.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHUPM_000580"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHUPM_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR0868461.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="ACHUPM_000581"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="ACHUPM_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR0868462.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="ACHUPM_000582"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="ACHUPM_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR0868463.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHUPM_000583"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHUPM_000583"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR0868464.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALREYVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHUPM_000584"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHUPM_000584"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR0868465.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="ACHUPM_000585"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="ACHUPM_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR0868466.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="ACHUPM_000586"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="ACHUPM_000586"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR0868467.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHUPM_000587"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHUPM_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR0868468.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHUPM_000588"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHUPM_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR0868469.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHUPM_000589"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHUPM_000589"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR0868470.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="ACHUPM_000590"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="ACHUPM_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHUPM_000591"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHUPM_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR0868471.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="ACHUPM_000592"
     CDS             complement(31717..33003)
                     /locus_tag="ACHUPM_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0868472.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="ACHUPM_000593"
     CDS             complement(33054..33995)
                     /locus_tag="ACHUPM_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR0868473.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="ACHUPM_000594"
     CDS             complement(34010..34780)
                     /locus_tag="ACHUPM_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR0868474.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35253..36767
                     /gene="caiT"
                     /locus_tag="ACHUPM_000595"
                     /pseudo
     CDS             35253..36767
                     /gene="caiT"
                     /locus_tag="ACHUPM_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
     gene            36798..37940
                     /gene="caiA"
                     /locus_tag="ACHUPM_000596"
     CDS             36798..37940
                     /gene="caiA"
                     /locus_tag="ACHUPM_000596"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR0868475.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            38069..39258
                     /gene="caiB"
                     /locus_tag="ACHUPM_000597"
                     /pseudo
     CDS             38069..39258
                     /gene="caiB"
                     /locus_tag="ACHUPM_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT         9397 a        10789 c        10279 g         8793 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tatgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcctg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaatgt
    35101 gataacaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    35161 cacgaggtta ataataatta tatcaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    35221 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    35281 tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg catactttgt tggcttacag
    35341 tcagagatct ggatgcagcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    35401 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    35461 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc acctgaattt agtaccgcca
    35521 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    35581 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacagggg
    35641 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    35701 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    35761 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggca
    35821 ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    35881 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    35941 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    36001 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    36061 tgggttgggt gttcattgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    36121 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    36181 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tatgctatcc
    36241 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    36301 gcatggtgct ggggctgaca gcgtcaacct ggatcctgtg gactgtactc ggtagtaaca
    36361 ctctgctgtt gatagataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    36421 tggcgcgcgc catcattgaa acctaggccg ctctgccact cagcaccgcc accatgtggg
    36481 gcttcttcat cctctgcttt attgccaccg ttacactggt taacgcctgc tcttataccc
    36541 tggcgatgtc cacttgccgc gaagtacgcg atggtgaaga accacctctg ctggtgcgta
    36601 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    36661 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    36721 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    36781 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    36841 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    36901 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    36961 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    37021 tggagctggg acgtctgggg gcaccaacct atgtgctgta ccagttgccg ggcgggttca
    37081 acaccttcct gcgcgaaggc acacaagagc agatcgacaa aattatggct ttccgcggca
    37141 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    37201 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    37261 ttattaccag cagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    37321 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    37381 ccaaacttga aaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgacg
    37441 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    37501 aagagttcga ccatgaacgt ttcctggtag ccctcaccaa ctacggtacg gcgatgtgcg
    37561 cttttgaaga tgcggcgcgc tacgccaatc agcgcgtgca gtttggcgag gctattggtc
    37621 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    37681 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    37741 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt tgtggatagc gcaatgcagg
    37801 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgatctgc
    37861 gtgtagaccg cgtctccggg ggatctgacg aaatgcagat cctgacgctg ggtcgcgcgg
    37921 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctaatg cgtcttatca
    37981 ggcctacggg agcacgaatg taggccggat aaagcgttta cgccgcatcc ggcagtcatg
    38041 cactacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcgggcc
    38101 gttggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccgggccgt tcgccgggca
    38161 aatgttcgca gaatggggtg cggaagttat ctggatcgag aacgtcgcct aggccgacac
    38221 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    38281 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    38341 cttcatcgaa gccagcaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    38401 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    38461 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    38521 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    38581 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    38641 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    38701 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    38761 ctactacgcc ggttgcggtc tgtataaatg cgccgacggc tacatcgtga tggaactggt
    38821 gggcattacc caaattgaag agtgctttaa agatattgac ctcgcacatc tactcggtac
    38881 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    38941 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    39001 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    39061 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    39121 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    39181 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    39241 cattcaggag ttggtcag
//
LOCUS       DAZPAC010000014        38337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZPAC010000014 DAZPAC010000000
VERSION     DAZPAC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.014"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            59..1423
                     /locus_tag="ACHUPM_000598"
     CDS             59..1423
                     /locus_tag="ACHUPM_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0868476.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHUPM_000599"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHUPM_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR0868477.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="ACHUPM_000600"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="ACHUPM_000600"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR0868478.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="ACHUPM_000601"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="ACHUPM_000601"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR0868479.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHUPM_000602"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHUPM_000602"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR0868480.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="ACHUPM_000603"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="ACHUPM_000603"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR0868481.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="ACHUPM_000604"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="ACHUPM_000604"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR0868482.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="ACHUPM_000605"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="ACHUPM_000605"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR0868483.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="ACHUPM_000606"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="ACHUPM_000606"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR0868484.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHUPM_000607"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHUPM_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR0868485.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHUPM_000608"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHUPM_000608"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR0868486.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHUPM_000609"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHUPM_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR0868487.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHUPM_000610"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHUPM_000610"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR0868488.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHUPM_000611"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHUPM_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR0868489.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHUPM_000612"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHUPM_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR0868490.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="ACHUPM_000613"
     CDS             13749..14288
                     /locus_tag="ACHUPM_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR0868491.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="ACHUPM_000614"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="ACHUPM_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR0868492.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHUPM_000615"
     CDS             complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHUPM_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR0868493.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHUPM_000616"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHUPM_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR0868494.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHUPM_000617"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHUPM_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR0868495.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHUPM_000618"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHUPM_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR0868496.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHUPM_000619"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHUPM_000619"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR0868497.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHUPM_000620"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHUPM_000620"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR0868498.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="ACHUPM_000621"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="ACHUPM_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFR0868499.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHUPM_000622"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHUPM_000622"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR0868500.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHUPM_000623"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHUPM_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR0868501.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHUPM_000624"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHUPM_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR0868502.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHUPM_000625"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHUPM_000625"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR0868503.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHUPM_000626"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHUPM_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR0868504.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHUPM_000627"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHUPM_000627"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR0868505.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHUPM_000628"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHUPM_000628"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR0868506.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="ACHUPM_000629"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="ACHUPM_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR0868507.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHUPM_000630"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHUPM_000630"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR0868508.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHUPM_000631"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHUPM_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR0868509.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="ACHUPM_000632"
     CDS             complement(37465..37920)
                     /locus_tag="ACHUPM_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868510.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="ACHUPM_000633"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="ACHUPM_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9471 a        10314 c         9728 g         8824 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaaacccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacac ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactac cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat caacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cactcccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcca
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc ccccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctatcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctagcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcacgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgcctggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacaa cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ctgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccaccag tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaggcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggat gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAZPAC010000015        36720 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZPAC010000015 DAZPAC010000000
VERSION     DAZPAC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36720
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.015"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHUPM_000634"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHUPM_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR0868511.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="ACHUPM_000635"
     CDS             complement(280..873)
                     /locus_tag="ACHUPM_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0868512.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="ACHUPM_000636"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="ACHUPM_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0868513.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="ACHUPM_000637"
     CDS             1788..2183
                     /locus_tag="ACHUPM_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0868514.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHUPM_000638"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHUPM_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR0868515.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHUPM_000639"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHUPM_000639"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR0868516.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHUPM_000640"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHUPM_000640"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR0868517.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="ACHUPM_000641"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="ACHUPM_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR0868518.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHUPM_000642"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHUPM_000642"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR0868519.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHUPM_000643"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHUPM_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR0868520.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="ACHUPM_000644"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="ACHUPM_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR0868521.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="ACHUPM_000645"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="ACHUPM_000645"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR0868522.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="ACHUPM_000646"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="ACHUPM_000646"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR0868523.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHUPM_000647"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHUPM_000647"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR0868524.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHUPM_000648"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHUPM_000648"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR0868525.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHUPM_000649"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHUPM_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFR0868526.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHUPM_000650"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHUPM_000650"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR0868527.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHUPM_000651"
     CDS             17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHUPM_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR0868528.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHUPM_000652"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHUPM_000652"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR0868529.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="ACHUPM_000653"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="ACHUPM_000653"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR0868530.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHUPM_000654"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHUPM_000654"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFR0868531.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHUPM_000655"
     CDS             complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHUPM_000655"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR0868532.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHUPM_000656"
     CDS             complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHUPM_000656"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR0868533.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHUPM_000657"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHUPM_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFR0868534.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="ACHUPM_000658"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="ACHUPM_000658"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR0868535.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="ACHUPM_000659"
     CDS             complement(26207..26548)
                     /locus_tag="ACHUPM_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR0868536.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHUPM_000660"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHUPM_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR0868537.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHUPM_000661"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHUPM_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR0868538.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="ACHUPM_000662"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="ACHUPM_000662"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR0868539.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="ACHUPM_000663"
     CDS             33227..34570
                     /locus_tag="ACHUPM_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR0868540.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34650..34856)
                     /locus_tag="ACHUPM_000664"
     CDS             complement(34650..34856)
                     /locus_tag="ACHUPM_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR0868541.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35181..35735
                     /locus_tag="ACHUPM_000665"
     CDS             35181..35735
                     /locus_tag="ACHUPM_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR0868542.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35825..>36720)
                     /gene="ytfI"
                     /locus_tag="ACHUPM_000666"
     CDS             complement(35825..>36720)
                     /gene="ytfI"
                     /locus_tag="ACHUPM_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR0868543.1"
                     /translation="HNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWISNYDSL
                     LTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLRGSGFEY
                     EEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQAIGDVS
                     LGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLGFALKIY
                     GGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEK
                     ICKKYKSTIEAFKKEQLALPV"
BASE COUNT         8893 a         9841 c         9130 g         8856 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcaa catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gacccctgaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcactttggt taagccatct ctgtttgcag acgcataacc
    34681 tgacggttaa cttcggacat cactgacaaa tgcagcttat ccttcacttt tgggataaga
    34741 atcttaccct tatcaaattc aaaagcgcca acgtccttga tgtataaccg tccacggaac
    34801 aggatcttta cgtacttcgc cacctgaagt gggttgtacc gttggaaaat tttcattgtt
    34861 atctcctgct gagtattacg cctgtgcggt gccacaatcg gcccaactat tatgaggcgc
    34921 aaattttaat gcttagtgac tatagactat ccgggcaatg tttccaccgc gtataacttt
    34981 ttttacctaa aggttacaat tattcagaat tatcttttta ccgaagcgcg gtcttcagta
    35041 taagcattca tttttcatat gatttgtgcg cttgaccgca aactggcatc acacttgcgg
    35101 gaaattcgat aaatagcaca tatgattaaa actcagaccc aagtggtcgg atcacctgca
    35161 tatcataaga aggaaacacc atgaccctac gcaagattct ggcactcacc tgcctgctgt
    35221 tgccgatgat ggcttccgca catcagttcg aaaccggtca gcgagtgccg ccgattggca
    35281 ttaccgatcg gggcgagttg gtgcttgata aagatcagtt tagctacaaa acctggaaca
    35341 gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa
    35401 aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc gaagttaccg cacgatcgtt
    35461 accagaccac cactattgtt aacaccgacg acgcgattcc gggttcaggg atgtttgtgc
    35521 gcagcagtct ggagagtaat aaaaagcttt atccctggtc gcagtttatt gtcgatagca
    35581 atggcgtcgc gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta gtggtgctgg
    35641 ataaggatgg tcgcgtgcaa tgggccaaag acggggcgct cactcaggaa gaggtgcagc
    35701 aagtgatgga cctgctgcaa aagttgctca aataatggca acaacataga ttctgcaaaa
    35761 ataattttcc cctatcgtta tctatgggat aggggaatta agtctctgag ttcttacgcc
    35821 gaactcatac tggtaacgct agttgctctt ttttaaacgc ctcaatggtc gacttatatt
    35881 tcttgcaaat cttttctttg gtaatattac tatctgcggc cagattcgca cttactatcg
    35941 catccacttg ttcggctaat tccacttgtt ggcaacctgc attaaccaga aaattataaa
    36001 taagggagta atcttcatcc tccaaaacaa cagaggaacc attacgaaaa cgttcatccc
    36061 ctccatagat tttaagcgca aagcccaaag attctatgac tatggcaaaa ttatcatcaa
    36121 ttattttttc gtgaatatac catccatcgc attctttaat tccacctcct ggtatttgga
    36181 gttcaaattt accccgtttt aatttgctta ctatatcctc caataaggca taacctaaag
    36241 acacgtcacc aatcgcctgg tgaatataac ctttaatcct tttcggtatg tcagactgtt
    36301 ttttctgcag aatatcacag gcatacaatc ttctgttttg ttctaacgga aactcgcggt
    36361 ttttgaaatc catgaatgag ttatactcat ttattgttat gggaatttct tcgtattcaa
    36421 atccactacc tcgcaatgtc attgtatatt cggcaccatt atctttaagg ctgaaactga
    36481 taaggaactt atccatggcg ttaacagttc cattttttga tgaaagtacc tgataactat
    36541 ttttatcaat ctgagttgcg caacgaaaga actccctcaa agttaacagg ctatcataat
    36601 tcgaaatcca atcttttttc ttaaatgcat gctggatatt gttaaaaaaa ttgtttgaat
    36661 gtgtttttgt atacgatgac tttacaaata actccaccgc accaataaaa ttattgtgtc
//
LOCUS       DAZPAC010000016        35251 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZPAC010000016 DAZPAC010000000
VERSION     DAZPAC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35251
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.016"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(282..1202)
                     /gene="malM"
                     /locus_tag="ACHUPM_000667"
     CDS             complement(282..1202)
                     /gene="malM"
                     /locus_tag="ACHUPM_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR0868544.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVSYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1354..2694)
                     /gene="lamB"
                     /locus_tag="ACHUPM_000668"
     CDS             complement(1354..2694)
                     /gene="lamB"
                     /locus_tag="ACHUPM_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR0868545.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(2766..3881)
                     /gene="malK"
                     /locus_tag="ACHUPM_000669"
     CDS             complement(2766..3881)
                     /gene="malK"
                     /locus_tag="ACHUPM_000669"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR0868546.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4246..5436
                     /gene="malE"
                     /locus_tag="ACHUPM_000670"
     CDS             4246..5436
                     /gene="malE"
                     /locus_tag="ACHUPM_000670"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR0868547.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            5590..7134
                     /gene="malF"
                     /locus_tag="ACHUPM_000671"
     CDS             5590..7134
                     /gene="malF"
                     /locus_tag="ACHUPM_000671"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR0868548.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7149..8039
                     /gene="malG"
                     /locus_tag="ACHUPM_000672"
     CDS             7149..8039
                     /gene="malG"
                     /locus_tag="ACHUPM_000672"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR0868549.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8411..9886
                     /gene="xylE"
                     /locus_tag="ACHUPM_000673"
     CDS             8411..9886
                     /gene="xylE"
                     /locus_tag="ACHUPM_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR0868550.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(9930..10340)
                     /gene="psiE"
                     /locus_tag="ACHUPM_000674"
     CDS             complement(9930..10340)
                     /gene="psiE"
                     /locus_tag="ACHUPM_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR0868551.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10466..10609)
                     /locus_tag="ACHUPM_000675"
     CDS             complement(10466..10609)
                     /locus_tag="ACHUPM_000675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868552.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            10610..10888
                     /locus_tag="ACHUPM_000676"
     CDS             10610..10888
                     /locus_tag="ACHUPM_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868553.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQSDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(10935..13031)
                     /locus_tag="ACHUPM_000677"
     CDS             complement(10935..13031)
                     /locus_tag="ACHUPM_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0868554.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13031..13768)
                     /locus_tag="ACHUPM_000678"
     CDS             complement(13031..13768)
                     /locus_tag="ACHUPM_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0868555.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(13765..14403)
                     /locus_tag="ACHUPM_000679"
     CDS             complement(13765..14403)
                     /locus_tag="ACHUPM_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR0868556.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(14517..14759)
                     /gene="yjbE"
                     /locus_tag="ACHUPM_000680"
     CDS             complement(14517..14759)
                     /gene="yjbE"
                     /locus_tag="ACHUPM_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR0868557.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15221..16870)
                     /gene="pgi"
                     /locus_tag="ACHUPM_000681"
     CDS             complement(15221..16870)
                     /gene="pgi"
                     /locus_tag="ACHUPM_000681"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR0868558.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      17107..17303
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17395..18744
                     /gene="lysC"
                     /locus_tag="ACHUPM_000682"
     CDS             17395..18744
                     /gene="lysC"
                     /locus_tag="ACHUPM_000682"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR0868559.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            18996..19268
                     /locus_tag="ACHUPM_000683"
     CDS             18996..19268
                     /locus_tag="ACHUPM_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR0868560.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19401..20273)
                     /gene="rluF"
                     /locus_tag="ACHUPM_000684"
     CDS             complement(19401..20273)
                     /gene="rluF"
                     /locus_tag="ACHUPM_000684"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR0868561.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            20621..21458
                     /locus_tag="ACHUPM_000685"
                     /pseudo
     CDS             20621..21458
                     /locus_tag="ACHUPM_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            21528..22334
                     /locus_tag="ACHUPM_000686"
     CDS             21528..22334
                     /locus_tag="ACHUPM_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0868562.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22344..22751
                     /locus_tag="ACHUPM_000687"
     CDS             22344..22751
                     /locus_tag="ACHUPM_000687"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR0868563.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            22751..23245
                     /locus_tag="ACHUPM_000688"
                     /pseudo
     CDS             22751..23245
                     /locus_tag="ACHUPM_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23311..24108
                     /locus_tag="ACHUPM_000689"
     CDS             23311..24108
                     /locus_tag="ACHUPM_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR0868564.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24119..24943
                     /locus_tag="ACHUPM_000690"
     CDS             24119..24943
                     /locus_tag="ACHUPM_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR0868565.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            24995..26223
                     /gene="sorE"
                     /locus_tag="ACHUPM_000691"
                     /pseudo
     CDS             24995..26223
                     /gene="sorE"
                     /locus_tag="ACHUPM_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            26606..27296
                     /gene="pepE"
                     /locus_tag="ACHUPM_000692"
                     /pseudo
     CDS             26606..27296
                     /gene="pepE"
                     /locus_tag="ACHUPM_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
     gene            complement(27387..29018)
                     /locus_tag="ACHUPM_000693"
     CDS             complement(27387..29018)
                     /locus_tag="ACHUPM_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR0868566.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29238..32921)
                     /gene="metH"
                     /locus_tag="ACHUPM_000694"
     CDS             complement(29238..32921)
                     /gene="metH"
                     /locus_tag="ACHUPM_000694"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR0868567.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33121..33945
                     /gene="iclR"
                     /locus_tag="ACHUPM_000695"
     CDS             33121..33945
                     /gene="iclR"
                     /locus_tag="ACHUPM_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR0868568.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34262..35251
                     /gene="espL4"
                     /locus_tag="ACHUPM_000696"
                     /pseudo
     CDS             34262..35251
                     /gene="espL4"
                     /locus_tag="ACHUPM_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT         8415 a         9135 c         9038 g         8663 t
ORIGIN      
        1 tcatgtttca tcaaagagtt attgccgcca gaatttgtga tatgacctat cataaaacct
       61 ctgtaataca gaaataaaat aacctggaaa agtagcctga gcgtccagac actaccggcg
      121 aaaggaaaag aatctctgat aaggcattga gataatggac attcttagtg aagaagaagt
      181 gaagtactat aaaaaatcgc tcatatccca ggaaggaagc ataaaaaaag gtgcgccagg
      241 agacgcacca gttgttgcaa aatcagcact gtggggcgta attacccctt gccttttaca
      301 ctgctgataa aggtggaacg ggcagatgtc gatcccaggc gttcagcttc atcaagcagt
      361 tttaacgcct tatcaacatc acctttcgcg acagcgtttt tgatcgcggt attaaaataa
      421 ctttccgtgt cgttgagcat cggctcgctt ttcttcaccg gcaccggagc aggtgcagcc
      481 acagctggtg ccgccgtgtt gcctaccgta accggagctg gagcggaaga accaaatagt
      541 ggtcctacca acacgctgga gctggagttc gttttgactt tcagtttcag taagccatcg
      601 ctggtatgac gagcaaccgg atcgggaata tccgggatcg agttaccgac gcctttggca
      661 taggctttag ccgggtcgag cagttgggtc gtctgctgga gatctttttc cgtggtaaag
      721 accagaacat aaagtttttg ctgccccaac gccggtgtca ggcgcataac gccttccagc
      781 cgatctgcac tcatcacgcc tggttcctgg taggtgaaat aactgctggg gaagaaggct
      841 gatggggtca tgttctgatc aagaatcagc acgttcggcg caaaaacgct ggtttgtttg
      901 ttcacttcgc tggtcagcgt cagggtcagt tcgccaatgt ttgccgggac gctgtacgca
      961 gcaaccggac cactgatgcc gggaacgttc agttgttggc cgccggtcgc cagttgggtg
     1021 gtctgggttt tagattgatc gaccggtgtc caggtgagtt gttgcagcgc ggcagatgga
     1081 atggctggcg cgtcgctggt gttttgcggt acgtagctaa catcggcaag gctaattcca
     1141 ggcgcgcttg ccagtaaccc tgctgataaa cagagggcga tgagactttt attcattttc
     1201 attgttttca cctcaaaatc tggagctcag cggtagccag gcaatagcgc gctaaacccg
     1261 ataatcagag gggctttcgc ccctttcaga taatgacaac ctgtttttat gccggatgcg
     1321 gcgtaaacgc cttatccggc ccaggttttg ctattaccac cagatttcca tctgggcacc
     1381 gaaggtccac tcgtcgctgt cgccacgacc gaagctgccg ccgttgaaat cagcaggaac
     1441 ggctttgccg tagttcgggt taaccttgct atcgccgttg tagtcgtaac cccatttctc
     1501 atcccacttg gcgtaggttg cgaagacacg aatagccggg cgtgaccaga tgctgtcgcc
     1561 agcctgccat tgttgtgcga gggtaatttt gtactgattg ttcttgtcgc cggtgcgctg
     1621 ggattcgacg ttgtcgtagc cgatttccat cacggtgctc atgattggcg tccacttgta
     1681 catcgggcga ataccgacgg tccaccactt ggtgccgttg tcgttatccc agttgatatc
     1741 ctggtacata cccacgtaca tcatgtccca gttgtcgccc atggagatcg caccgtggtc
     1801 gaggatacgc agcatgtgac cgttgttgtt gatattgtag gcaaattttt cgttatcaaa
     1861 cgcgacgcca gaaccctgcg acagaccttt accctgcgag gtcatcgagt cagtagcgta
     1921 ctgaacaaca aacttgttaa agcccttcag gacactctga gtatgttcag cagtgaataa
     1981 ccagccgtct ttcgatgcgc catcaaccag acgatagtta tcacgcaggt tggcacgacc
     2041 gtagtcgaca cccagttcta atgtgccgcc cgggttgatt tccatctgcg ctaaacgcac
     2101 atcgaaaacg tcgttcgcgg tttcgttggt atagtcataa atattgttgc tggcaaaaga
     2161 ggaagaacca ccagcttcag aggagcgggt tgctgccaga gagagtttac cgaagccaac
     2221 atcgatgttt tccagaccgg caccaggacc agaaatatcc cagtagtaga agtcgatcat
     2281 atgaacgtca tgacgttggt agaagcgctt acctgcccag atggtggaac ctggcagcca
     2341 ttcgatcagg tttttaccct gcacgtttgc ttcacggaag gccggatcgg tagcttccca
     2401 gtcattctgt tgcgcgacgg aataggccac gttagtgtcg aaatagaagc tcttatcgcc
     2461 ctctttccac acttcctgac ccaattttaa ttcagcataa gtttcacatt cgttgccaag
     2521 acggtattta ctttgagcac cggtagtctg gaaacactgt tgttcaccgc cgctacctgt
     2581 ccagccaata ccggaacgtg catagccgtg gaaatcaaca gccattgcct gagcagacat
     2641 tacgcccgct gcgacggcaa ccgccagagg aagtttgcgc agagtaatca tcattctatc
     2701 tcctgagtca ttgcttttct tttttcacat cacctgtgac aggctttgtg tgttttgtgg
     2761 ggtgcttaaa cgcccggctc cttatgcagt cgacgacatg cagtgccatc ctcacggaac
     2821 agatggcaac gctctggcgg caggccgata gcgaatgtgg caccttcttc taccaacacc
     2881 acgtcgttct ggcggtacac caggttttga cgaatggaag ggatctggat atggatttgg
     2941 gtttcgttgc cgagttgctc gacgacctga acttcaccct caaggatgac gtcagcgata
     3001 tcactcggca gtagatgttc cgggcgaata cccagcgaca tattggctcc aacctggaca
     3061 tcacggcttt caactggcag ccagacttgc tgacgatttg gcatcggcag ctccacctgc
     3121 acttgatcga ttgcggtggc ggtcactttt accggcagga agttcatctt tggcgaaccg
     3181 ataaatccgg cgacaaaacg gtctgccgga tagtggtaca gctccagcgg tttcccaacc
     3241 tgcgccacgc gaccggcgtc cagcaccacg attttgtcgg ccagcgtcat cgcttcgacc
     3301 tgatcgtggg tgacgtaaat cattgtgcgg cccaggcgtt tatgcagacg ggagatttcg
     3361 atacgcattt gcacacgcag tgcagcatcg aggttggaga gcggttcatc gagcaaaaat
     3421 acgcttggct cggccaccag cgtacggcca atcgccacac gctgacgctg accaccggag
     3481 agcgctttcg gtttgcgatc cagcaaatgc gccagttgca gcacttccgc cacctggttg
     3541 acgcgttggt taatcacctc tttttttgcg ccagccagtt tcaggccaaa tgacatgttt
     3601 tctgctactg acaggtgggg atagagcgcg taagactgaa acaccatacc aacgccgcgt
     3661 tctgctggcg gagtgtcatt catccgtttc tcaccgatga acaggtcgcc gctggtgatc
     3721 gtctcaagcc cggcaatcat gcgcagtaaa gtcgatttac cgcagccaga cggtccgaca
     3781 aacaccacga attcaccttc atggatatcg agattgatat ctttcgatac cacgacctcg
     3841 ccccaggctt tcgttacatt ttgcagctgt acgctcgcca tgcccttctc cctttgtaac
     3901 aacctgtcat cgacagcaac attcatgatg ggctgactat gcgtcatcag gagatggctt
     3961 aaatcctcca ccccctggct tttttatggg ggaggaggcg ggaggatgag aacacggctt
     4021 ctgtgaacta aaccgaggtc atgtaaggaa tttcgtgatg ttgcttgcaa aaatcgtggc
     4081 gattttatgt gcgcatctcc acattaccgc caattctgta acagagatca cacaaagcaa
     4141 cggtggggcg taggggcaag gaggatggaa agaggttgcc gtataaagaa actagagtcc
     4201 gtttaggtgt tttcacgagc acttcaccaa caaggaccat agattatgaa aataaaaaca
     4261 ggtgcacgca tcctcgcatt atccgcatta acgacgatga tgttttccgc ctcggctctc
     4321 gccaaaatcg aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt
     4381 ctcgctgaag tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat
     4441 ccggataaac tggaagagaa attccctcag gttgcggcaa ctggcgatgg ccctgacatt
     4501 atcttctggg cgcacgaccg ttttggtggc tacgctcaat ctggcctgtt ggctgaaatc
     4561 accccggaca aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac
     4621 aacggcaagc tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa
     4681 gatctgctgc cgaacccacc aaaaacctgg gaagagatcc cggcgctgga taaagaactg
     4741 aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg
     4801 ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgatattaaa
     4861 gacgtggccg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgaccttatt
     4921 aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa
     4981 ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa
     5041 gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatctaa accgttcgtt
     5101 ggcgtgctga gcgccggtat taacgccgcc agtccgaaca aagagctggc gaaagagttc
     5161 ctcgaaaact atctgctgac tgacgaaggt ctggaagcgg tcaataaaga caaaccgctg
     5221 ggtgcagtgg cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc
     5281 accatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc
     5341 tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa
     5401 gccctgaaag acgcgcagac tcgtatcacc aagtaatgct gtgaaatgcc ggatgcggcg
     5461 tgaacgcctt gtccggccta caaaactgaa acgcatgtag gcctgataag acgcatcagt
     5521 gtcgcatcag gcagttgttg tcggataagg cgtgaaagcc ttatccgtcc tggaatgagg
     5581 aagaacccca tggatgtcat taaaaagaaa cattggtggc aaagcgacgc gctgaaatgg
     5641 tcagtgctag gtctgctcgg cctgctggtg ggttaccttg ttgttttaat gtacgcacaa
     5701 ggggaatacc tgttcgccat taccacgctg atattgagtt cagcggggct gtatattttc
     5761 gccaatcgta aagcctacgc ctggcgctat gtttacccgg gaatggctgg aatgggatta
     5821 ttcgtcctct tccctctggt ctgcaccatc gccattgcct tcaccaacta cagcagcact
     5881 aaccagctga cttttgaatg tgcgcaggaa gtgttgttag atcgctcctg gcaagcaggc
     5941 aaaacctata actttggtct ttacccggcg ggcgatgagt ggcaactggc gctcagtgac
     6001 ggcgaaatcg gcaaaaatta cctctccgac gcgtttaaat ttggcggcga acaaaaactg
     6061 caactgaaag aaacgaccgc ccagcccgaa ggcgaacgcg cgaatctacg cgtgattacc
     6121 cagaatcgtc aggcgctgag tgacattacc gccattctgc cggatggcaa caaagtgatg
     6181 atgagctccc tgcgccagtt ttctggcacg cagccgctct acacactcga cggtgacggc
     6241 acgttgacga ataatcagag cggcgtgaaa tatcgtccga ataaccaaat tggcttttac
     6301 cagtccatta ccgccgacgg caactggggt gatgaaaagc taagccccgg ttacaccgtg
     6361 actactggct ggaaaaactt tacccgcgtc ttcaccgacg aaggcattca gaaaccgttc
     6421 ctcgccattt tcgtctggac cgtggtgttc tcgctgatca ctgtcttttt aacggtggcg
     6481 gtcggcatgg ttctggcatg tctggtgcag tgggaagcat tgcgcggcaa agcggtctat
     6541 cgcgttctgc tgattctgcc ctacgcggtg ccatcattca tttcaatctt gattttcaaa
     6601 gggttgttta accagagctt cggtgaaatc aacatgatgt tgagcgcgct gtttggcgtg
     6661 aagcccgcct ggttcagcga tccgaccacc gcccgcacga tgctaattat cgtcaatacc
     6721 tggctgggtt atccgtacat gatgatcctc tgcatgggct tgctgaaagc gattccggac
     6781 gatttgtatg aagcctcagc aatggatggc gcagatccgt tccagaactt ctttaagatt
     6841 acgctgccgc tgctgattaa accgctgacg ccgctgatga tcgccagctt cgcctttaac
     6901 tttaacaact tcgtgctgat tcaactgtta accaacggcg gcccggatcg tcttggcacg
     6961 accacgccag ccggttatac cgacctgctt gttaactaca cctaccgtat cgcttttgaa
     7021 ggcggcgggg gtcaggactt cggtctggct gcagcaattg ccacgctgct cttcctgctg
     7081 gtgggtgcgc tggcgatagt gaacctgaaa gccacgcgaa tgaagtttga ttaagggaga
     7141 taacaaaaat ggcaatggtc caaccgaaat cgcaaaaagc acgtttgttt attactcacc
     7201 tgctactgct actttttatc gcggcgatta tgttcccgct gctgatggtc gtcgctatct
     7261 cactgcgcca ggggaacttt gcgatcggca gcctgatccc ggagcaaatc tcctgggatc
     7321 actggaaact ggctttaggt tttagcgtgg aacaggctga tggtcgcatt acgccaccgc
     7381 cattcccggt actgctgtgg ctatggaact cggtaaaggt cgccgggatt tccgcgattg
     7441 gcattgtcgc gctctctacc acctgcgcct acgctttcgc ccgtatgcgc tttccaggca
     7501 aagcgacgct gctgaaagga atgctgattt tccagatgtt cccggcagta ctttcactgg
     7561 tcgcgttgta tgcgttgttt gatcgtctgg gtgagtacat tccattcatt ggcctgaata
     7621 ctcacggcgg cgtaattttc gcgtatctgg gtgggattgc gctgcatgtc tggaccatca
     7681 aaggctattt cgaaaccatc gatagttcgc tggaagaagc ggctgcgctg gatggtgcga
     7741 caccgtggca ggccttccgc cttgtcctgt tgccgctgtc agtaccgatt ctggcggtgg
     7801 tattcatcct gtcgtttatc gctgccatta ctgaagttcc ggtcgcgtcg ctgttactgc
     7861 gtgacgtaaa cagctacacc ctggccgtgg ggatgcagca atacctcaac ccgcaaaact
     7921 acctgtgggg tgactttgcc gccgctgccg tgatgtctgc attaccgatc accatcgtct
     7981 tcttgctggc tcaacgctgg ctggtcaacg gcctgacggc aggtggtgtg aaaggttaaa
     8041 gatgttgttc tgccaatgtt atgccgctgc accctcaact tacgttatcc caacttgtga
     8101 ctgttattcg gcgctccacg gagcgccttt ttttctttcg tctgcaatct gaatcgttcg
     8161 ccggttaata tttccatcat agagcttatt atttttacgt tatttgtttt cccacttacg
     8221 ataattctct ttcgtgctct gagtcacggc aatagtattg ttttcatcaa tttttgataa
     8281 ttatcacaat taagatcaca gaaaagacat tacgtaaacg cattgtaaaa aatgataatt
     8341 gccttaactg cctgacaatt ccaacatcaa tgcactgata aaagatcaga atggtctaag
     8401 gcaggtctga atgaataccc agtataattc cagttatata ttttcgatta ccttagtcgc
     8461 tacattaggt ggtttattat ttggctacga caccgccgtt atttccggta ctgttgagtc
     8521 actcaatacc gtctttgttg ctccacaaaa cttaagtgaa tccgctgcca actccctgtt
     8581 aggattttgc gtagccagcg ctctgattgg ttgcatcatc ggcggtgccc tcggtggtta
     8641 ttgcagtaac cgcttcggcc gtcgtgattc acttaagatt gctgctgtcc tgttttttat
     8701 ttctggtgta ggttctgcct ggccagaact tggttttacc tctataaacc cggacaacac
     8761 agtacctatt tatctggcag gttatgtccc ggaatttgtt atttatcgta ttattggcgg
     8821 tattggcgtt ggtttagcct caatgctttc accaatgtat attgcggaac tggctccagc
     8881 tcatattcgc ggaaaactgg tttcatttaa ccagtttgcg attattttcg ggcaactttt
     8941 agtttactgc gtaaactatt ttattgcccg ttccggtgat gccagctggc tgaatactga
     9001 cggctggcgt tatatgtttg cctcggaatg tatccctgca ctgctgttct taatgctgct
     9061 gtataccgtg ccagaaagtc ctcgctggct gatgtcacgc ggcaagcaag aacaggcgga
     9121 aagtatcctg cgcaaaatta tgggcaacac gcttgcaact caggcagtac aggaaattaa
     9181 acactccctg gatcatggcc gcaaaaccgg tggtcgtctg ctgatgtttg gcgtgggcgt
     9241 gattgtcatc ggcgttatgc tctccatctt ccagcaattt gtcggcatca atgtggtgct
     9301 gtactacgcg ccggaagtgt tcaaaacgct gggggccagc acggatatcg cgctgttgca
     9361 gaccattatt gtcggagtta tcaacctcac cttcaccgtt ctggcaatta tgacggtgga
     9421 taaatttggt cgtaagccac tgcaaattat cggcgcactc ggaatggcaa tcggtatgtt
     9481 tagcctcggt accgcgtttt acactcaggc accgggtatt gtggcgctac tgtcgatgct
     9541 gttctatgtt gctgcctttg ccatgtcctg gggtccggta tgctgggtac cgctgtcgga
     9601 aatcttcccg aatgctattc gtggtaaagc gctggcaatc gcggtggcgg cccagtggct
     9661 ggcgaactac ttcgtctcct ggaccttccc gatgatggac aaaaactcct ggctggtggc
     9721 ccatttccac aacggtttct cctactggat ttacgggtgt atgggcgttc tggcagcact
     9781 atttatgtgg aaatttgtcc cggaaaccaa aggtaaaacc cttgaggagc tggaagccct
     9841 ctgggaaccg gaaacgaaga aaacacaaca aactgctacg ctgtaatctt cctgtccagc
     9901 acgccgcgcc agttcggcgt gctttctttt tactcccgct tcagccgttt cgaattacac
     9961 agccacagag tgatcaccag taacaggatc gcagccgagt agatcaacac atccagtggc
    10021 gatttatgat cgacgatgat caagcgcaca atggcggtga tcccaatata gacaaagtaa
    10081 cgtagcggga agtgaaaacc ggactgaaag tacttcacaa tcagcgcgat aaattcgaaa
    10141 tagagaaagt aaaccaccag tccttctacc agctcatatt tgctggtttg ttctggcgcg
    10201 aacagcacat ctgccagatg caccgtttct ttgccgagga agacaaccaa aatcaggccg
    10261 aggcacagca ggccaagatt gagtacggtc tgcaaaatgg tggagataaa ctccacgcgc
    10321 ggacgagaga gtgacgtcat agcagtacct ccttcacagt ggcgagcttc ctgtataacg
    10381 caaaaatgtg acggagatct atattttttg tgtatgtttt gttcaacctg gatttggagc
    10441 gcgcctccac caaagccgga tatacctacc cgtaggccgg ataagccgcg cctggcggcg
    10501 catccggcaa gacaaaccgc acccaagccg gtaattcaaa aatcacccat tcaggtgatt
    10561 cccagccacc agcaatgcgc gaaaaatcga caatgttcct ctggaacaaa tgaaacgtaa
    10621 cctgataaag gtggtaaaaa tgaagcctta ttttgctgct ttgatgttat cagtctctgt
    10681 tcttcctgct tatgctggtc cgctcggtac tgctgataaa gccgacctac cgcaaagcaa
    10741 tgtctccagc ccaatgatgg cccagtcgct caggcaatca gacctgcaac cgatatcgac
    10801 ggacaggaag acagaatgtt ttcgccttta cacaccagat cgcaaaccgg gggtgaactg
    10861 cgttcctgat ggctcaacag gccattaaga atgagggtgc cagtgattgc gaaaactggc
    10921 acctttccca tgacttagcg gaaattgacg ctacggtcgc tggtcatatc atacaactgg
    10981 aacttacgcc caagttgctg accaccgtca cgcgtcagcg gcgtccagcc aatcgccgca
    11041 cggctgcgtg tcgggccaga cgagaagaga tccaacggta ccgagacata cacgcctttg
    11101 gtgaagtccc cttcgccgta ctcctctttt gaaacattcg tgatcgtggc atagccaccc
    11161 accaccacgc cgctatcaaa gcgtttggcg atctccagcg tgccgccttt atcccctgcc
    11221 agatactgcc cgacgctggc tttcactaac acgtcctggg cgaaagatgg cgtccagtag
    11281 gcggtcagat gtccggtttt cacgctgtag tcggtgaatt tcatcatatc ttttgcacta
    11341 cgccagtcgc gctgtttaac gtagttggca tccagaccaa acgcccagtt gctgtccagc
    11401 ggacgataca acacttcagc ccccgcaccg ccaaacatgg tttcgagata acctccatag
    11461 acctgaccgt agaagccgtt gcccagatgc tggaagtagt tggcttgcag gttattcaca
    11521 taggcatcgt tctgcacata ctcgcgcaca tgggtacgaa cgcgcggtaa gtgcgagtcc
    11581 tgtggcggat tggtgtagtt aaacttgtcg tagttgttgg caagatttgc aaacaggctg
    11641 ccgatggtca gcagatggtc cgtcagccac aaatctgccg ttcccatcac gcccagctga
    11701 tacatgtaaa agttttccgg gccaccgacc gactggttca gcaccggatc gatatggaaa
    11761 tcaaagcgtg atttgtcgat ataccagccc tgctcggtgg actgcggaac cactggctcg
    11821 acgcgttttt gcgccagcgt cgtttcgtgg cccaacggct ctccggcgag atgatttttc
    11881 aggctggcga catcggtttc cgtcgtcacc tgcggcatgt taaggcgatt ttccgtaatg
    11941 cggatcgttt tgatcccatc cggcagatcg ttcatcacga tccgattagc acggatgatc
    12001 ccttcgcgcg aatcacgata tttcacctgc tcgccggtaa catacagcgt atcgcctttc
    12061 gcctggatct gcggatcggc aagtccggca ttgtatttca gcaacgttaa ctgattcgcc
    12121 accaccgagt gctgcaaaat ggcatcctgc ggctgcggtt gatattgcgg gcgggcgtta
    12181 tcgatgtagg acgggcgcag atcgttaaag ttggtgcgca gcgtgacgcc aaacataaag
    12241 gtgttgccac gttcatagct aaggttaacg tcggcccaat cggtaacgcg ataaatcgcc
    12301 ccgacgttaa acttactttt ttgctccagc ttcccggcaa aatcctgctg ataattatta
    12361 ccttcatact ccagtttcag gcgcagcggt tgccagggcg tctggtattc cacgccgcca
    12421 aacagtgagg ccgggccgtg gaacatctga ctgccgtcga tagatcccgc ttgtttgtag
    12481 ctgttatcgc gatagcaata tttatcactg gctgaacaga gcggattttt cacattaccg
    12541 ctggtgccca gatatcccca gcccaggccg agcgtaaaat cgaacggccc ccaggctttg
    12601 ctggcaacaa gatattccgc atcaaacagc cccgtaccgc cgatatcccg cgcaccaacc
    12661 gccacttgcg gcagccagta actctcttcc cacaaacgca gtttgagatc gaaggcttta
    12721 tctttatacg tttgatcgcc agagaacgct tcgacgctgc tgtactgccg ggtgcgcacg
    12781 tcggtgtaac gcagcgttgt ttccagccac gggaagagtt gcactgaagc tgagtaataa
    12841 cggtactgat cgttatcgcg atagttcaga ctcaactccc cttcccgtgc catacgcgcg
    12901 gtgggagttt gtaataatcc tacgccaccg aaatccgact gcgacggacc aatgggcgca
    12961 ggatatgttt cgccgtagca agctgtgcta atgcccagcg ccagtaagct aagcagatgt
    13021 ctttttttca ttattggggt atccgctgcg tcagagtctg aagaatgtcg gcgttaaggg
    13081 catcaggcgt ctcactccag acggagtctg cgaggccaac ataaataatg ctgccgggca
    13141 tcggctctac gtgacgcttg ttccagtaag ccaccggcgc tttttgcgtg cgtccgtccg
    13201 ggtaaacgac ccacgcgtag ctgcgatccg caccgctgag caggctctgg tcagagagat
    13261 agctcgccac gtcgcgacct ggagtgaatg gctgattgcc aggatggctg ataagcccga
    13321 acaacgtgac cgtggacggc ggtggtccga cccacagcgt gtagttgccc tgcaacggcg
    13381 ggttaccgcg ttcggcaacg cggacgatat cgggatcaag attgattttt tgcctgcctg
    13441 tcacttccaa tgcctggatt tgctggcgta aggcgttaat ggcggcggca tcgtcggtgc
    13501 tggaatctgc gccctgttct gccagccgtg tcagcaacgc ctgctgctga cgcaatgctg
    13561 ccgccgttgc cagctcttcg ctaatcaccg caccaggcca ccagctgttt gccagtcgag
    13621 gttgccctac cagatcgatt aaatgctctg cgcccgttag cgttttggcc tcactgctgc
    13681 cattgctgaa caccttgacg gttcctgccg cgaagaccga tgacgctccc acactcaaaa
    13741 gcaacgcgac aatagtttgt ttaatcattg ttttgccgcc ttgatgagag tggttttcac
    13801 cgggaaataa tccgcgccga gatactgttc cgactggcga atttgccctt cgctgtcgat
    13861 ccagtagcgg ttatgccagc gcgcctggtc ggtggagact tcttcgtcca gcacgcgaac
    13921 cagggtttca tcgctgccga ccttgacggt atcggtgcca tcccatttga agactgagcg
    13981 tgcggtggcg tagcgtacct gctggtactc ggtccagccc atcgtgcgcg tccatgttgc
    14041 accatcaaca atttgtgcgg gtttaatcag cgggtcggcg gcaaggttat tcacttcaat
    14101 caggttgtcg ccgccaagca aagtcttcac cagacgacca tgttgtgtga caagagtggc
    14161 ttgatcctga gtgacccatt tttgttgtcc gtcttcagcg aaggccagca ccacaaacaa
    14221 ttgcggcccg ccattaagct gcatgtactg gctggcgtag ggcatatttt gaatatcatc
    14281 gtccgtcagc tgtacgcctg gcgtgccgaa cagactgtcc cacagtgaat tgcccagctc
    14341 tttagtcgtg gctgaacagg cctgtaataa caggcagata agaatgagtg caggtcgctt
    14401 cacgactctt ctccagaggg gcagaataac cacaccggag tgtggttatg gttaatccct
    14461 tcatatttca agccacagat gcaatgactc gaattatttt ggggatacat actttattac
    14521 tgggtactgg tggttgtggt tgttgtggtc ccggtattag aaccatcacc gccaccggtt
    14581 gccgcgagcg cgacacccac cgccgagctt acggtgctga cgctggtcgc ggaggaactc
    14641 cccgccgaaa ccgacgttgc tgccgcccct gccgcttcgc ccacctgtac aggtgcagcc
    14701 cacacagaag tcgccgcaag cgcagatatg gcaaaaatgc catacagaac ttttttcata
    14761 acaatttccc ttcattgaat gaatggagat ttacccaaaa acatttcggg cgggatcgag
    14821 tatacacaac taaagcatgc gaattacggc gcggggaatt agcgatggtg ttttaggata
    14881 attggaatga atatcaaata taagtttcaa cttatattta aataacaata aaaatcataa
    14941 acttaaaata aaaacaaaag taattaatct gcgtattctc cgaaataaac aacgattaaa
    15001 aatctatttt aggattaacc tcacggtatg atttccgtta aatcacagac aaggggattt
    15061 atctgataaa acaatgccga cttattgtcg gcattgtttt taaggttatt taactcctca
    15121 tactatgcat taatgtcgat attggcatca ggctccggtt gccggatgcg gcgtaaacgc
    15181 cttatccggc ctacatatcg gcatcaggca cgcatgatga ttaaccgcgc cacgctttat
    15241 agcggttaat cagaccattg gtcgagctat cgtggctgct gatttctttg tcatctttca
    15301 gctctggcag aatacggttc gccagttgtt tacccaactc cacgccccac tggtcgaagg
    15361 taaagatgtt caggatcacg ccctgagtaa agattttgtg ctcatacagc gcaatcaacg
    15421 cacccaggct gaacggagtg atttcgcgca gcaggatgga gttggtcggg cggttacctt
    15481 cgaacacttt gaacggcacc acgtagtcaa gcgttgccgg atctttaccc tgatcgcgat
    15541 attcctgctc aaccacttcg cgggatttac caaacgccag cgcttcggtc tgggcgaaga
    15601 agttagacag cagtttctgg tggtgatcag agagcgggtt atgggtgata gccggggcga
    15661 tgaaatcgca cggtaccatt ttggttccct ggtggatcag ctggtagaac gcgtgctgac
    15721 cgttagtgcc tggttcaccc cagataatcg ggccagtctg gtaatccaca acgttaccgt
    15781 tacggtcaac atacttaccg ttggactcca tattgccctg ctggaagtac gccgcgaaac
    15841 ggtgcatata ctggtcatac ggcagaatcg cttcagtttc cgcaccaaag aaattgttgt
    15901 accagatgcc aatcagcgcc agcaggacag gcaggttttt ctcggcaggc gtggtggaga
    15961 aatgcttgtc catcgcgtgt gcgccagaaa gcagttcaac gaagttatca aagccgatgg
    16021 agagaacaat cgacaggcca atcgctgacc acaaagagta acggccgccg acccagtccc
    16081 agaactcgaa catgttggca gtatcaatac caaactcgcc aacggctttg gcattggtgg
    16141 aaagcgccgc aaagtgtttt gcaacgtgtt tttcatcacc tgccgctttc aggaaccagt
    16201 cacgcgcgct atgggcgttg gtcatagttt cctgagtggt gaaggtttta gatgctacca
    16261 ggaacagcgt ggtttccggg tttacttttt tcagcacttc agcgatgtga gtcccatcga
    16321 cgttagaaac aaagtgcatg ttcaggtgat ttttgtacgg acgcagtgct tcggtcacca
    16381 tgtacgggcc gaggtcagaa ccaccgatcc cgatgttcac tacgtcagtg attgctttgc
    16441 cggtataacc cttccactca ccggagataa tcgcttccga gaaggttttc atcttctcca
    16501 gcaccgcgtt gacttccggc attacgtctt tgccatcaac cagaatcggg gtattgctac
    16561 ggttacgcag cgctacgtgc agcacggcgc ggttttcagt gcggttgatc ttctcgccag
    16621 agaacatcga cttaatcgcg cccgccagat cgcactcttt cgccagatcc tgtaatttcg
    16681 ccagcgtctc ttcagtgatg cggtttttgg agtaatccac cagcatctga tcgtcgaatg
    16741 ttgcggagaa cttagaaaaa cgatcgccgt ctttagcaaa aagatcggcg atcgtaacgt
    16801 ctttcatttc atcgaagtgt ttctgtagtg cctgccaggc agcggtctgc gttggattga
    16861 tgtttttcat tagcaatact cttctgattt tgagaattgt gactttggaa gattgtagcg
    16921 ccagtcacag aaaaatgtga tggttttagt gccgttagcg taatgttgag tgtaaaccct
    16981 tagcgcagtg aagcatttat tagctgaact actgaccgcc aggagtggat gaaaaatccg
    17041 catgaccccg tcgttgacaa ccgccccgct caccctttat ttataaatgt actacctgcg
    17101 ctagcgcagg ccagaagagg cgcgttgccc aagtaacggt gttggaggag ccagtcctgt
    17161 gataacacct gagggggtgc atcgccgagg tgattgaacg gctggccacg ttcatcatcg
    17221 gctacagggg ctgaatcccc tgggttgtca ccagaagcgt tcgcagtcgg gcgtttcgca
    17281 agtggtggag cacttctggg tgaaaatagt agcgaagtat cgctctgcgc ccacccgtct
    17341 tccgctcttc ccttgtgcca aggctgaaaa tggatcccct gacacgaggt agttatgtct
    17401 gaaattgttg tctccaaatt tggcggtacc agcgtagctg attttgacgc catgaaccgc
    17461 agcgctgata ttgtgctttc tgatgccaac gtgcgtttag tagtcctctc ggcttctgct
    17521 ggtatcacta atctgctggt cgctttagcg gaagggctgg aacctggcga gcgattcgaa
    17581 aaactcgacg ctattcgcaa catccagttt gccattctgg aacgtctgcg ttacccgaac
    17641 gttatccgtg aagagattga acgtctgctg gagaacatta ctgttctggc agaagcggcg
    17701 gcgctggcaa cgtctccggc gctgacagat gagctggtca gccacggcga gctgatgtcg
    17761 accctgctgt ttgtcgaaat cctgcgcgaa cgcgatgttc aggcacagtg gtttgatgta
    17821 cgtaaagtga tgcgtaccaa cgaccgattt ggtcgtgcag agccagatgt agccgcgctg
    17881 gcggaactgg ccgcgctgca gctgctccca cgcctcaatg aaggcttagt gatcacccag
    17941 ggatttatcg gtagcgaaaa taaaggtcgt acaacgacgc ttggccgtgg aggcagcgat
    18001 tatacggcag ccttgctggc ggaggcttta cacgcatctc gtgttgatat ctggaccgac
    18061 gtcccgggca tctacaccac cgatccacgc gtagtttccg cagcaaaacg cattgatgaa
    18121 atcgcgtttg ccgaagcggc agagatggca acttttggtg caaaagtact gcatccggca
    18181 acgttgctac ccgcagtacg cagcgatatc ccggtctttg tcggctccag caaagaccca
    18241 cgcgcaggtg gtacgctggt gtgcaataaa actgaaaatc cgccgctgtt ccgcgcgctg
    18301 gcgcttcgtc gcaatcagac tctgctcact ttgcacagcc tgaatatgct gcattctcgc
    18361 ggtttcctcg cggaagtttt cggcatcctc gcgcggcata atatttcggt agacttaatc
    18421 accacgtcag aagtgagcgt ggcattaacc cttgatacca ccggttcaac ctccactggc
    18481 gatacgttgc tgacgcaatc tctgctgatg gagctttccg cactgtgccg ggtggaagta
    18541 gaagaaggtc tggcgctggt cgcgttgatt ggcaatgacc tgtcaaaagc ctgcggcgtt
    18601 ggcaaagagg tattcggcgt actggaaccg ttcaacattc gcatgatttg ttacggcgca
    18661 tccagccata acctgtgctt cctggtgccc ggcgaagatg ccgagcaggt ggtgcaaaaa
    18721 ctgcatagta atttgtttga gtaaatactg tatggcctgg aagctatatt tcgggccgta
    18781 ttgattttct tgtcactatg cttatcaata aacgagcctg tactctgtta accaacgtct
    18841 ttatcgagaa taattgcctt taattttttt atctgcatct ctaattaatt atcgaaagag
    18901 ataaatagtt aagagaaggc aaaatgaata ttatcagttc tgctcgcaaa cgaattcccg
    18961 tacactgaac tctctggctc actgagggca ccatcatggc actcccccgt attacccaaa
    19021 aagagatgac tgaacgcgag cagcgtgaac ttaaaacact gctggatcgg gcgcggattg
    19081 cgcatggtcg cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata
    19141 aattaatggt cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg
    19201 cttataaacc ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca
    19261 ggcgctaatg aacaatcaac gcctgagatt ttcactcgtc agtgtgaaca caattttccc
    19321 ctcaccctaa cccttttccc gaaggggcga ggggactgtc cgagcacttt tgggccgttt
    19381 tcatcagtct gaggcgttaa tcagcgcccc ttctttttac gccccggcga ggtaaaacgc
    19441 ttaccgttgg acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga
    19501 cgtttgatgc ccgctgtttt cggtttggcc ttcgctttgg gcttcgcctc ggaagaggaa
    19561 ttttcaatga gcttaaagag atcgataagc tcgtcgtcgg ttaaatcgcg ccattctccc
    19621 agcggaatgc cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca
    19681 tagccgaaat gctcgcacat gcgccggatc tgacggttca acccctgcac cagggtaatg
    19741 cggaagacaa acggtgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc
    19801 ggcactcccg cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc
    19861 agatactctt tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga
    19921 ttggtgagga aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt
    19981 ttgctgtggt taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca
    20041 ataccgacgg gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc
    20101 aactgaccgt ttactttcac aatgtcgccg ggtttcacct gatcaccaat ggtggctcgc
    20161 ttgccattaa ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa
    20221 attccgcttt cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct
    20281 cctgtaaaac gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac
    20341 gtcgaaatta aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat
    20401 gcaaatttgc ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc
    20461 tgatgactac tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa
    20521 agagtgaaaa atcgctttga aaggccctca taatggggtt atatcagatc aagcggtgag
    20581 aaagcgtatt tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat
    20641 ccgtttgatt gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat
    20701 cgcgcgcgaa ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga
    20761 tcagggaatt gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca
    20821 gcaactgaag caaaagtttg gcttgaaaga cgttgtggtg gtgtcgggaa atgatgagga
    20881 tgaagagact caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct
    20941 ggagcctggc gatattgtcg gtttttccta gggtcgcgcg gtgagcgcac tggttgaaaa
    21001 cttgccgcaa gcggggcaat cgcggcagtt aatctgcgtg ccaattattg gcggaccgtc
    21061 cggtaaactc gaaagccgct atcacgtaaa cacattaacc tacagcgtgg cagcgaagct
    21121 gaaaggggaa tcgcatctcg cggattttcc ggctctgctg gataacccct taattcgtaa
    21181 tgggatcatg cagtctcagc actttaaaac catctctgcc tactgggata atctggatgt
    21241 cgccctggtg ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta
    21301 tggtggtgaa gagagtgacg acctgaaagc aggcgcggta ttccattggc attgccatga
    21361 gtgaagaaaa atacagcgga attgttggtg cactgcgtgg aaaatatatt aattgtctgg
    21421 taacgaatag cagcacagct gaactattac tgaaataaaa atcttcccga tgtttaccgt
    21481 aataaaaatt acggcgggtc cctattatct aaaatcagga gtctgttatg caaacgtggt
    21541 taaatttgca ggataaaatc attatcgtca ccggcggcgc atccggtatt ggtctggcga
    21601 ttgtagagga attattagca caaggcgcaa acgtacagat ggtagatatt cacggtggcg
    21661 atggtcaata tgaagttcat aaaggctatc agttttggcc gaccgatatt tccagcgcca
    21721 aagaggtaaa tcatacggta gcggaaatta tccagcgttt tggtcgcatc gacggtctgg
    21781 tcaataacgc cggggtcaat ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc
    21841 agtatgaact caacgaagct gcattcgaaa aaatggtcaa tatcaaccaa aaaggcgttt
    21901 ttctgatgtc gcaggcggtg gcgcgccaga tggtcaaaca acatgatggc gtgattgtga
    21961 atgtttcctc agaaagtgga ctggaaggct cagaaggcca aagctgttac gccgctacca
    22021 aagccgcgct caatagcttc acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc
    22081 gtgtggtcgg tatcgcgccg gggattctgg aaaaaaccgg gctacgcacg ccggaatatg
    22141 aagaggcgct ggcgtggacg cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca
    22201 aaaacgccat tcctattggg cgcgccggaa gattagcaga agtcgctgat tttgtttgtt
    22261 atctgctgtc tgaacgcgcc agctacatca ccggagtaac cactaacatt gcgggcggca
    22321 aaacgcgcgg ataaggaggc tttatggtca acgccatttt ttgtgcccac ggcaaattag
    22381 cttgcgccat gctggaatcg gttcagatgg tgtacggcga cgccaatgtc gaggcggttg
    22441 aatttgtacc aggcgagaac gccggagaca tcgtagcaaa gctggaaaag ttagtaagca
    22501 ttcacaatca agatgagtgg ctgattgccg tggatttaca gtgcggcagc ccgtggaatg
    22561 cggcagcgat gcttgcgatg cgcaatcctc gtctacgggt aatcagcggc ctttccctgc
    22621 cgctggcgct cgaactggtt gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac
    22681 acctgacgca aatcgcaaag caaacctgcg ttgtctggaa acaactggca acggcagagg
    22741 aagatttctg atgaacatta cgctcgcccg cattgatgac cgtctgatcc acggccaggt
    22801 cactaccgtc tggtcgaaag tggctaacgc ccagcgcatt attatctgca atgacgaagt
    22861 ttataacgat gaagtgcgcc ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt
    22921 taacgtggtc aatattgaaa aagccgtcgc ggtttatcac aacccgcaat attaggacga
    22981 aaccgttttt tatcttttca ctcgcccaca ggatgcctta gcgatggttc gccagggggt
    23041 aaaaataggc accctgaata ttggcggcat ggcctggcgg cccggtaaaa aacagttaac
    23101 caaagcggtt tcattagatg atgatgatat taacgcattt catgagttaa ataatcttgg
    23161 cgtcataatg gatttacgcg tcgtagcgtc agatccctca atcaatatta tcgacaaaat
    23221 aaacgaacag ttaatcgcaa attaaataat agcgcctgtg tacatatgct ttaacaggca
    23281 gggatgacga cacttaaaag gtgacaaatc atggaaataa gtaccctaca aattattgct
    23341 atatttcttt tttcctgtat tgccggaatg ggcagcgtgc tggatgaatt tcagactcat
    23401 cgcccgttaa ttgcctgtac ggtgattggt ttaattctcg gtgatttaaa aaccggaata
    23461 atactcggtg gtacgctgga attgatagct ctcggctgga tgaacgtcgg cgcggcgcaa
    23521 tctccggatt ctgcactcgc cagcataatc tccgccattc tggttatcgt tggtcagcaa
    23581 agcatcgcca ccggaatcgc catcgcgttg cctgtggctg cggcaggcca agtgctgacg
    23641 gtgtttgccc gtaccatcac cgtagtgttc cagcacgcgg cggataaagc agcggaagag
    23701 gcgcggtttc gcactctcga tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc
    23761 gttgctattc cggcactgat tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg
    23821 ttaaacgcca ttcccgaatt tgtgacccgt ggactgcaaa ttgctggcgg ttttatcgtg
    23881 gtggtcggtt acgccatggt gctacgtatg atgggcgtga aatatttgat gcctttcttt
    23941 ttcctcggtt tcctcgcagg tggctacctc gatctcagcc tgctggcgtt cggtggcgtc
    24001 ggcgtgatca tggccctggt ctacatccag ctaaatccac agtggcgtaa agctgaacct
    24061 caactccagg ccaccgcctc caccgccctt gaccaacttg acgattaacg gagccatcat
    24121 ggaacagaga aaaattacac gcagcgatct ggtgagcatg tttctgcgct ccaacctgca
    24181 acaggcgtcc tttaactttg aacgtattca cggactgggc ttttgctacg acatgatccc
    24241 cgccatcaag cgactttacc cattaaaaga ggatcaggtt gcggcgctca ggcgacacct
    24301 ggtgttcttc aataccacgc cagccgtatg tggcccggtc atcggcgtca ctgccgccat
    24361 ggaagaggcg cgggccaacg gcgcggaaat tgatgacggt accattaacg gcatcaaagt
    24421 cggtctgatg ggaccattgg caggagttgg cgatccactg gtctggggaa cgctgcgccc
    24481 gattaccgct gcgctcggcg catctctggc actttctggc aacatcctcg gcccgctgct
    24541 gtttttcttt attttcaatg cggtgcgtct ggcgatgaag tggtatggcc tacagctcgg
    24601 ctttcgcaaa ggggtgaata tcgtcagcga tatgggcggg aatgtgctgc aaaaactcac
    24661 cgaaggcgcg tcgattctcg gactgtttgt gatgggcgtg ctggtcacca aatggacgtc
    24721 aatcaacgta ccgttggtgg tttcacaaac gcatgccgcc gatggctcca ccgtcatcat
    24781 gaccgtgcag aacattctcg accaactttg ccctggtttg ctggcgctcg gtctgacgct
    24841 actgatggtt cgtctgctca acaaaaaaat taacccggta tggctgattt tcgccctgtt
    24901 tggcttaggg attatcggca atgcgctggg cttcctgtcc tgattcttcg ccccggcacg
    24961 actgccgggg ccatcgctca acatgaggtg gtttatgaaa acaacagctc tgcgtcttta
    25021 tggtaaacgt gatttacgcc tggaaacctt tgaccttcct gaaatgcagg aggatgaaat
    25081 cctcgcgacg gtggtcactg acagcctgtg cctctcttcc tggaaagagg caacctgggt
    25141 gaaaaccata aaaaagtacc cgacgatgtg gcgaccaacc caatcatcat cggccacgag
    25201 ttttgcggcg atattctggc cgtgggtaaa aagtggcagc acaaattcca gccgggtcag
    25261 cgttatgtga ttcaggccaa cctgaaactc cctgaccgcc cggactgccc cggctactcc
    25321 ttcccgtggg tgggcggcga agccacgcat gtggttattc ccaacgaggt catggaacaa
    25381 gattgcctgc tggcatacga cggcgaaacc tattttgaag gctcgctggt tgaaccgctt
    25441 tcctgcgtga ttggcgcgtt caacgccaac tatcatcttc aggaaggtag ttataaccac
    25501 acgatgggga ttcgcccgca agggcgcacg ctgatcctcg gcggcaccgg accaatggga
    25561 ttgttggcga ttgattatgc gctgcatgga ccagttaacc cgtcgctgct cgtcattacc
    25621 gataccgaca acgacaaatt gagttatgcg cgcaagcact atccatcaga accgcaaaca
    25681 ctgattcatt atctcaatgc caccgatgca gcatttgata cgttaatggc gctgagtggc
    25741 ggtcacggct tcgatgatat tttcgtcttt gtgcctaatg aaggactggt gactctcgcc
    25801 tcttccttgc tggcgacaga tggttgcctg aatttcttcg ccggaccgca ggataaacat
    25861 ttcagcgcgc caattaattt ctacgatgtg cattatgcat ttacccacta cgtgggcacg
    25921 tcaggcggca ataccgacga catgcgcgcg gcggtcaaac tgattgaaga gaaaaaagtg
    25981 caggccgcaa aagtggtaac acatattctt gggctgaatg ccgcgggcga aaccacgctt
    26041 gaattgcctg ccgtcggcgg cggcaaaaag ctggtgtata ccgggaaata cctgccgctg
    26101 acgtcactca cgcagattca ggatcaagca ctggcggcga ttctggcgcg tcatcagggg
    26161 atctggtccg gtgaggcgga gcaatatctg ctcactcatg cagaggcaat ttcccatgat
    26221 taatcgcgct acttaactct gcatgtcgct ggctggtcgc cgcggcaact tcgcgacctg
    26281 atgtgcggct gattgctgaa gcggcccaat gtgcacgaga aggataaaaa aacatcagcg
    26341 ccttaagggg cgctcaaatt ggtggcaaag tacacattgt ttatgccgga tgcggaataa
    26401 agccttattc ggcctacata atctcgaaaa ttcaatatat tgcagttatc tggtaggcct
    26461 ggtaagcgta gcgcatcagg caatgctgtg tttgtcatca gtctcagcgc ccggtgcaat
    26521 acgtgacatc gtgagccatg tcagtaagtg gttaaattcg cagtggtagc atgccttccg
    26581 tgatttctta ctaaccggag aagtaatgga actgctttta ttgagtaact cgacgctgcc
    26641 gggtaaagcc tggctggaac atgcactgcc gctaattgct gaacagttgc agggtcgccg
    26701 ctcagcggtg tttatccctt tcgctggcgt aacgcagacc tgtgatgatt acacagcgaa
    26761 aacggctgcg gttctcgctc cgctgggtgt ttctgtcacc ggtattcata gcgttgtcga
    26821 tcccgttgcc gcgattgaaa atgctgagat cgtgattgtc ggggggcggg aatactttcc
    26881 agttgctgaa acagtgccgc gagcgcgggc tgctggcacc aattactgac gtggttaaac
    26941 gtggtgcgct gtatattggc tggagcgcag gcgctaacct tgcttgccca accattcgta
    27001 ccaccaacga tatgccgatt gtcgatccgc aaggtttcga tgcgctaaat ctgttcccgc
    27061 tgcaaatcaa cccgcacttc accaacgcgc tgccggaagg ccataaaggt gaaacccgtg
    27121 agcagcgtat tcgcgaactg ctggtcgtcg cgccagaact gacgattatt ggtctaccgg
    27181 aaggtaactg gatcacggtg agtaaaggtc acgctacgct gggtggcccg aacaccactt
    27241 atgtgtttaa ggccggtgaa gaagcggtgc cgctggaagc gggtcaccgt ttttaagtga
    27301 attgccggat gcatgatccg gcatcttgtt catagtgcct gatgcgacgc ttgacgcgtc
    27361 ttatcaggcc tacaggattc acaatattaa tactcgtccc gtccttcatc ttcatccggc
    27421 tgttccagca cactgtaagc caccgaacaa aacagcgagt tcagacgctg catatctccc
    27481 agtaagccta aatgtagcga actggtttca atgctctgca cgttttgctg atgcaggcga
    27541 tcgacgtggg cgtgggaata gcggcgatta agaatgcgaa agcgatgctt gctgcggcgc
    27601 aaacgacgag cgctggtgac atcgccagag aagaacaccg acattgccag ctttaaatta
    27661 ctgagcaatt gctcatagag cgcatccagt tccttcaacc catcaagcga aaatgcccgc
    27721 cgtgctgcca gcgatttatc agcaatttcg ctgcccatgc gctcgacgat atcggaagcc
    27781 tgttcaaggt tgagcgacat ttcaataatc tccgcccagc gacgcgactc ttcttctgcc
    27841 agttcctctt ttggcattcg cgccagatac agcttaatgg cggtgtagag aacgttgata
    27901 tcatctgcca gcttacgcag ctctttctcc tgccgtggct cgccgtgcat cactttattc
    27961 agcccttcca tcatctgttc catggcgtcg ccaatgcgca gggtttcgcg cgcggcgttc
    28021 gccagagcaa gcgtgggcgt atccagcgcg ctgacatcca gatgtttagg ccgtagctgg
    28081 gtatccagtt ccggttcatc gcgaataatc gttttgcaaa accgtgccat cgggtcaaca
    28141 aatggcagca tgaccaggca acgtacaagg ttgtagaaga cgtggaaata gatcaccagt
    28201 tccgcttttg gcaatgacaa cttccccatt gtctctgcca gcaaatggac aaacggcagg
    28261 ataatcaggc tacccaccag cttaaacagc aaactaccca gcgcgacacg gcgggctgcg
    28321 gcattggcgg cactgttgtt gagcatcgcc agcaggccgg aaccgaggtt agcaccaatc
    28381 accagacaga gcgccacggg gaaggagata atgcctgcgg cggtcagagt cgcggtcagc
    28441 agtacagcag caaggctgga gtagctgata atggcgaaca tcgcgccaat cagcgcatcc
    28501 agcagaatat cgccggtcag cgaggcaaag atcacctgaa cgccgtttgc ctgggtgatc
    28561 ggcgttacgg cctgcacaat caactccagc gccagcaaaa tcagcccaag accaataccg
    28621 acgcggccca gttgcccggc gcgtgactgt ttgcgtccga ggaaaaaaat cacgccgata
    28681 aaaataagta acggtgacag ccaggataag tcgaaggtga gaatacgcgc cattagcgcc
    28741 gtcccgacat ctgcacccag cacaatcacc agagccggtg cgagggctac cagatcctga
    28801 gcgacgaacg aggtcaccag catggtggtg gcattactgc tctgtaccag tgcggtaacg
    28861 ccgatccccg cgcaaaaggc gagcggcttt ttttcgacgc tccggctaag gacagtacgc
    28921 aaacgcgcgc cgaagacgcg cattacgccg gttcgaacaa tatgagtccc ccagaccagc
    28981 agggcgacgg cagaaagcag gtgaagcagc gttaacacga gttcaggttc tccttatcgt
    29041 tatatgttcc tgaagcaatt gaatgaccaa aggaccggaa acacgctgac tggcatcttt
    29101 actgtgcctt ttgacattcg tttttccggc taagattatt ccagcctgtt tccagtataa
    29161 gtgtttcata ttaataaaga gacagggcac tcattgagcg ccctgtttgt tgtaaggaaa
    29221 agtgacagat ttgtgaatca gtccgcgtca taccccagat tcggtgccag ccagcgctca
    29281 acatcggaga cgctcatacc tttacggcgg gcatagtctt caacctgatc gcgctgaatt
    29341 tgcgccaccg cgtagtactt gctgtccggg tggctgaaat accaacccga aaccgatgca
    29401 ccaggccaca tggcgaaaga ttctgtgagt ttcatgccag tatgtttttc cacttccagc
    29461 agctcccaga tggtggcttt ttccgtatgt tccgggcagg ccgggtagcc cggtgccgga
    29521 cggatgccct ggtagttttc gcggatcagt tcttcgttgc tgaggttctc gttcggcgca
    29581 tagccccagt agactttacg cacacgctca tggagatact ccgcaaaggc ttcggctaaa
    29641 cggtcggcaa gcgctttcac catgattttg ttgtaatcat cgtgctgcgc ttcaaaggca
    29701 tcagccagtg cgtcctcttc cagcccgcca gtcacggcaa atgcgccgat gtaatctgct
    29761 ttaccagaaa gcttcggcgc aacgaagtca gcgagacagt agttagcgaa tcccgttttt
    29821 tcggtctgtt gacgcagatg gtggctgacg ttgatcacat gggtgcgcgt ttcgtcacgg
    29881 tagatttcaa tgtcatcgcc cacacggttt gccgggaaca agcccaccac gccacgcgga
    29941 ttcagcgttt tctcggcgct taatttatcc agcatgtcgt tggcgtcttt aaacagccgc
    30001 tgcgcctcaa cgcccaccac ttcatcttcc agaatgcgcg gatacttccc ggccagcgac
    30061 caggtcataa agaacggtgt ccagtcgatg taattacgca gagtttcgat gctggcttca
    30121 acttcctgca cgccgagacg gtgcgccacc ggcggcgtgt aagcctgcca gtcaaaagcg
    30181 aagtcgttat cgcgcgccgc ttccagcgtg accggtggtg tgcgcggttt cttgcgcccg
    30241 tgctgaatac gtacggtttc atactccttg cgggtacgag cgacaaaatc atcgcgctga
    30301 gtatcggaaa gcagcgccgc caccacacca acggtgcgcg aggcattctg cacatacacc
    30361 gtcgggccgc tgtagttctg ctcaattttc accgccgtgt gcgcttttga ggtcgtcgcg
    30421 ccaccgatca gtaacggaat ggtgaagccc tgacgctcca tctctttcgc cacgttaacc
    30481 atctcgtcca gcgacggcgt gataagcccc gaaagtccaa tcagatcagc attcacttct
    30541 ttagcggtac ggagaatttt ttccgcaggc accataacgc cgagatcgac aatttcgtag
    30601 ttgttacatt gcagcaccac accaacgata tttttaccga tgtcgtggac gtcgcccttc
    30661 acggtggcga tcaccatctt gccgttggtt ttgccctgct ctttgctggc ttcaataaac
    30721 ggttcgaggt aggccactgc ctgtttcatg acgcgcgccg atttgaccac ctgtggcagg
    30781 aacattttcc cctcgccaaa cagatcgccg accacgttca tgccgtccat caacgggcct
    30841 tcaatcactt caatcgggcg cgtagcctgc tggcgggctt cttcggtatc ctgctcgata
    30901 aactcagtaa tgcctttgac cagcgagtat tccagacgtt tattcacttc ccacgagcgc
    30961 cactccgcct gctgggcgtt agcggtgtcg tcggttttgc tgccgcgata tttctcggca
    31021 agctccagta aacgttcagt gccatcatcg cgacgattaa gaatcacatc ttccaccgcg
    31081 tcgcgcagtt cagcgggcag gtcgtcgtaa atcgccagtt gcccggcgtt gacgatcccc
    31141 atatccatgc cattgcgaat agcgtagtag aggaacaccg cgtgaatggc ttcgcgcacc
    31201 ggttcgttgc cacggaatga gaaagaaacg ttagatacgc cgccggaaat cagcgcgtgc
    31261 ggcagttcgc gtttgatgtc ttcgcaggcg ccgataaagt cctgcgcata gttgttgtgc
    31321 tcttcaatgc cggtggcgac cgcgaagatg ttcgggtcga agatgatatc ttctggcggg
    31381 aagccaacct cttcggtgag gattttgtac gcccgacggc aaatctcgat tttccgtgcg
    31441 cgggtatcgg cctgtccctg ttcgtcaaag gccattacca ccaccgctgc accgtaacga
    31501 cgcaacagtt tcgcatgatg gataaaggcg tcgacgccct ctttcattga gatagagtta
    31561 acaatgcctt tgccctgaat acacttcagc cccttttcga tgacctccca ttttgaggag
    31621 tcgatcataa tcggcacgcg agcgatatcc ggttcaccgg caatcagatt gagaaaacgc
    31681 accatcgccg cttcggcgtc gagcatccct tcatccatat tgatatcgat aatctgcgcg
    31741 ccgttttcca cctgctggcg ggccacatcc agtgcctcgc tgtatttctc ttctttgatc
    31801 aggcgcttga acttagcgga accggtgacg ttggtgcgtt cacccacgtt cacaaacagg
    31861 ctatcttcgc caatgttcag cggctccagg ccggacaaac ggcaggctac ggggatttcc
    31921 ggcagtttgc gcggcgctaa tccttctact gcacgactca tcgctgcaat atgttgtggc
    31981 gtggtgccac agcagccgcc gacgatattg agaaagcccg cttccgccca ttcacgtatt
    32041 tgttttgcca tcgtgtcggc gtcgagatcg tactcaccaa aggcgttggg tagcccggcg
    32101 ttcgggtgcg cggtgacgta gcattccgca atccgtgaca gctcctgcac gtactggcgc
    32161 agttcatcgg gccccagcgc acagttcagg ccaaaggtca gcgcttcggc gtggcgcaat
    32221 gagttgtaaa atgcttcggt ggtctgcccg gagagcgtgc gcccggaggc gtcggtgatg
    32281 gtgccagaga tcataatcgg cagctcaacg cccagcgttt caaactccgt ttttaccgca
    32341 aataccgccg ctttggcgtt aagggtgtcg aaaacggttt caatcaggat cagatccgca
    32401 ccaccttcca ccagcgcttt ggtggactct cgataagcca ccaccagccc gtcaaaagtg
    32461 atattacgaa atgccggatc gttgacgtcc ggagaaatag acgccgtgcg gttggttggg
    32521 ccgagaacac cggcaacgta gcgcggtttc tccggcgtgc gcgcggtcca ctcgtcagca
    32581 caggcgcgcg ccagtttcgc cgccgcaaag ttgatttccg ccgacaggga ttccatctgg
    32641 taatccgcca tcgcaatggt cgtggagttg aaggtgttgg tttcgatgat atccgcgccc
    32701 gcttcaaagt aggcgttgtg gatagcggcg atcacttccg gtttactgag taccagcagg
    32761 tcgttgttgc ctttgaggtc gcatggccag tcggcaaagc gttcaccacg aaaatcggct
    32821 tcgttcagtc gatagctctg gatcatggtg cccataccgc cgtccagcac cagaatacgt
    32881 tcatttaact gcgcacgcag ttgttccact ttgctgctca cacttgctcc cgacacgctc
    32941 aacccagatt taataaaaat tcaacaaacc atactggcat aaacgcatct gtggtaaaag
    33001 cgaccaccac gcaacatgag atttgttcaa cattaactca tcggatcagt tcagcaacta
    33061 ttgcattaac taacaataaa aatgaaaatg atttccacga tacagaaaaa ggagactgtc
    33121 atggtcgcac ccattcccgc gaaacgcggc agaaaacccg ccgttgccac cgcaccagcg
    33181 actggacagg ttcagtcttt aacgcgtggc ctgaaattac tggagtggat tgccgaatcc
    33241 aatggcagtg tggcactcac ggaactggcg caacaagccg ggttacccaa ttccacgacc
    33301 caccgcctgc taaccacgat gcaacagcag ggtttcgtgc gtcaggttgg cgaactggga
    33361 cactgggcaa tcggcgcaca tgcctttatg gtcggcagca gctttctcca gagccgtaat
    33421 ttgttagcga ttgttcaccc tatcctgcgc aatctaatgg aagagtctgg cgaaacggtc
    33481 aatatggcgg tgcttgatca aagcgatcac gaagcgatta ttatcgatca ggtacagtgt
    33541 actcacctga tgcgtatgtc cgcacctatc ggcggtaaat tgccgatgca cgcttccggt
    33601 gcaggtaaag cctttttagc ccaactgagc gaagaacagg tgacgaagct gctgcaccgc
    33661 aaagggttac atgcctatac ccacgcaacg ctggtgtctc ctgtgcattt aaaagaagat
    33721 ctcgcccaaa cgcgcaaacg gggctattca tttgacgatg aggaacatgc actggggcta
    33781 cgttgccttg cagcgtgtat tttcgatgag caccgtgaac cgtttgccgc aatttctatt
    33841 tccggaccga tttcacgtat taccgatgac cgcgtgaccg agtttggcgc gatggtgatt
    33901 aaagcggcga aggaagtgac gctggcgtac ggtggaatgc gctgactttt tctggcgggc
    33961 agaggcaata ttctgcccat catacctgag tggcaataga ataagggtgt ctgttaatcg
    34021 cattgacgcc aaaataactt aatgtcatac acttcacaca cttttattaa ctcagcatta
    34081 tttttaaaca tcaaaccact taattattaa caaaatcgca agatctcatt ccaccgaaac
    34141 ttttacggct gcattatgag attgagcgat ttatgatatt tctttagttg taatcatatt
    34201 aaattttttc actgttctga tagttaaaat ttaagacatc aataaacaat gagatattta
    34261 aatgattact cgtattcctc gtagttcttt ctctgcaaat attaataata cagcccagac
    34321 aaatgaacac caaaccctga gtgaattgtt ttataaagaa ctcgaggata aattttctgg
    34381 caaggagctg gcgacgcctc tattaaaaag cttctcagag aactgtagac aaaatggtcg
    34441 tcatatcttt agcaacaagg attttgtcat taaattttcc acgtccgtct tacaagctga
    34501 taagaaagaa attacgataa ttaataaaac gaaaacacga cactcactca aaccattgct
    34561 ccaatatttg aagaatacct aatggaaatt ttacctcaac gctcagacac tcttgataaa
    34621 caagaattaa acctaaaatc agatagaaaa gaaaaagaat tcccaagaat taaacttaat
    34681 ggtcaatgct attttccggg gcgaccccaa aaccgtatag tatgccgaca cattgctgca
    34741 caatatatta atgatattta tcagaatgtt gattacaaac cccatcaaga tgattattct
    34801 tcagctgaaa aatttctcac tcacttcaac aaaaaatgca aaaaccagac tttggcgttg
    34861 atttccagcc gtcctgaggg gcgttgcgtt gctgcctgcg gtgatttcgg gctagttatg
    34921 aaagcatatt ttgacaagat ggaatcaaat ggcatcagtg ttatggcagc catattactg
    34981 gtggataacc atgctttgac ggtccggcta agaataaaga acacaactga aggatgtacc
    35041 cattacgtgg tttcggttta tgatcctaat gtaactaacg acaaaataag aatcatgagc
    35101 gaaagcaaag aggatattaa acactattct ctgatggatt ttatgaatgt agattatagc
    35161 ctcctgaaat ggtcaaatga tcatgttatt aaccaatctg ttgcaataat tccagcactt
    35221 ccgaaagaac agctattgat attaaaagga t
//
LOCUS       DAZPAC010000017        34982 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZPAC010000017 DAZPAC010000000
VERSION     DAZPAC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34982
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.017"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHUPM_000697"
     CDS             complement(<1..815)
                     /locus_tag="ACHUPM_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0868569.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHUPM_000698"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHUPM_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHUPM_000699"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHUPM_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1397..1873)
                     /locus_tag="ACHUPM_000700"
     CDS             complement(1397..1873)
                     /locus_tag="ACHUPM_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR0868570.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHUPM_000701"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHUPM_000701"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR0868571.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="ACHUPM_000702"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="ACHUPM_000702"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR0868572.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="ACHUPM_000703"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="ACHUPM_000703"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR0868573.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="ACHUPM_000704"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="ACHUPM_000704"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR0868574.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6911
                     /gene="bioD"
                     /locus_tag="ACHUPM_000705"
     CDS             6234..6911
                     /gene="bioD"
                     /locus_tag="ACHUPM_000705"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0868575.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7490..9511
                     /gene="uvrB"
                     /locus_tag="ACHUPM_000706"
     CDS             7490..9511
                     /gene="uvrB"
                     /locus_tag="ACHUPM_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR0868576.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="ACHUPM_000707"
     CDS             complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="ACHUPM_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR0868577.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10876..11020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11008..11997
                     /gene="moaA"
                     /locus_tag="ACHUPM_000708"
     CDS             11008..11997
                     /gene="moaA"
                     /locus_tag="ACHUPM_000708"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR0868578.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12019..12531
                     /gene="moaB"
                     /locus_tag="ACHUPM_000709"
     CDS             12019..12531
                     /gene="moaB"
                     /locus_tag="ACHUPM_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR0868579.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12534..13019
                     /gene="moaC"
                     /locus_tag="ACHUPM_000710"
     CDS             12534..13019
                     /gene="moaC"
                     /locus_tag="ACHUPM_000710"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR0868580.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13012..13257
                     /gene="moaD"
                     /locus_tag="ACHUPM_000711"
     CDS             13012..13257
                     /gene="moaD"
                     /locus_tag="ACHUPM_000711"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR0868581.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13259..13711
                     /gene="moaE"
                     /locus_tag="ACHUPM_000712"
     CDS             13259..13711
                     /gene="moaE"
                     /locus_tag="ACHUPM_000712"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR0868582.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13848..14552
                     /locus_tag="ACHUPM_000713"
     CDS             13848..14552
                     /locus_tag="ACHUPM_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR0868583.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14823..15470
                     /locus_tag="ACHUPM_000714"
     CDS             14823..15470
                     /locus_tag="ACHUPM_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR0868584.1"
                     /translation="MWLITALGLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLC
                     MYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISMLAG
                     LLFNVEPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRNK
                     LLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(15535..16246)
                     /locus_tag="ACHUPM_000715"
                     /pseudo
     CDS             complement(15535..16246)
                     /locus_tag="ACHUPM_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
     gene            complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="ACHUPM_000716"
     CDS             complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="ACHUPM_000716"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR0868585.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17484..18245)
                     /locus_tag="ACHUPM_000717"
     CDS             complement(17484..18245)
                     /locus_tag="ACHUPM_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0868586.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18378..18788
                     /locus_tag="ACHUPM_000718"
     CDS             18378..18788
                     /locus_tag="ACHUPM_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR0868587.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18750..19856)
                     /locus_tag="ACHUPM_000719"
     CDS             complement(18750..19856)
                     /locus_tag="ACHUPM_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0868588.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19867..21000)
                     /locus_tag="ACHUPM_000720"
     CDS             complement(19867..21000)
                     /locus_tag="ACHUPM_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0868589.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="ACHUPM_000721"
     CDS             complement(20993..22729)
                     /locus_tag="ACHUPM_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0868590.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHUPM_000722"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHUPM_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR0868591.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHUPM_000723"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHUPM_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR0868592.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHUPM_000724"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHUPM_000724"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR0868593.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHUPM_000725"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHUPM_000725"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR0868594.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="ACHUPM_000726"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="ACHUPM_000726"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR0868595.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHUPM_000727"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHUPM_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR0868596.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHUPM_000728"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHUPM_000728"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR0868597.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHUPM_000729"
     CDS             31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHUPM_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR0868598.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHUPM_000730"
     CDS             complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHUPM_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR0868599.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHUPM_000731"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHUPM_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR0868600.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHUPM_000732"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHUPM_000732"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR0868601.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34982)
                     /locus_tag="ACHUPM_000733"
                     /pseudo
     CDS             complement(32997..34982)
                     /locus_tag="ACHUPM_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8273 a         8977 c         9488 g         8244 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggatct gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatccagaaa atgcggcaac cggaaagtac ataaaccttg
     6901 ccttgttgta gccattctgt atttggttaa attgcgagcg agatcgcgtc ttcgattgac
     6961 tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa aattttactt ctgcctcatg
     7021 cggcgaatgt gggaattgcc caggcggcgg gggatagggg ctggagacag ttatccacta
     7081 ttcctgtgga taaccatgtg tattagagtt agaaaacacg aggcaagcga gagaatacgc
     7141 ggcttgcacg cgaattggcg ttaaagacgg ctcaaagaaa tatcttttat tttttaactg
     7201 gttagataaa tgcaatggca gtcactgaac aggcatctct tgccataaaa ctgtcatcac
     7261 tcatcttgac aaatgttaaa aaagccgttg ctttggggat aacccggtaa ggccggagtt
     7321 ttatctcgcc acagagtaaa ttttgctcat gattgacagc ggagtttacg ctgtatcaga
     7381 aatattatgg tgatgaactg tttttttatc cagtataatt tgttggcata attaagtacg
     7441 acgagtaaaa ttacatacct gcccgcccaa ctccttcagg tagcgactca tgagtaaacc
     7501 gttcaaactg aattccgctt ttaaaccttc tggcgatcag ccagaggcga ttcgacgtct
     7561 cgaagagggg ctggaagatg gcctggcgca ccagacgtta cttggcgtga ctggctcagg
     7621 gaaaaccttc accattgcca atgtcattgc tgaccttcag cgcccaacca tggtacttgc
     7681 gcccaacaaa acgctggcgg cccagctgta tggcgaaatg aaagagttct tcccggaaaa
     7741 cgcggtggag tatttcgtct cctactacga ctactatcag ccggaagcct atgtaccgag
     7801 ttccgacact ttcattgaga aagatgcctc ggttaacgaa catattgagc agatgcgttt
     7861 atccgccacc aaagcgatgc tggagcggcg tgatgtggtt gtagtggcct ctgtttccgc
     7921 gatttatggt ctgggcgatc ctgatttata tctcaagatg atgctccatc tcacggtcgg
     7981 tatgattatc gatcagcgcg cgattctgcg ccgactggcg gagctgcaat acgctcgtaa
     8041 tgatcaagca ttccagcgtg gtactttccg cgttcgtggc gaggtgatag atatcttccc
     8101 ggcagaatcg gatgacattg cacttcgcgt ggaactgttt gacgaggaag tggaacgatt
     8161 gtcgttattt gacccgctga ccgggcagat tgtttccact attccacgtt ttaccatcta
     8221 cccgaaaacg cactacgtca caccgcgcga gcgcatcgta caggcgatgg aggagatcaa
     8281 agaagaactg gccgccagac gcaaagtgct gttggaaaac aacaaactgc tggaagagca
     8341 gcggctgacc cagcgtaccc agtttgatct ggagatgatg aacgagctgg gctactgttc
     8401 ggggattgaa aactactcgc gcttcctctc cggtcgtgga ccgggtgagc caccgccgac
     8461 gctgtttgat tacctgcctg ccgatgggct gctggtggtc gatgaatctc acgtcaccat
     8521 tccacaaatt ggcggcatgt atcgcggtga ccgggcgcgt aaagagacac tggtggagta
     8581 cggcttccgc ctgccatcag cgctggataa ccgtccgctt aagtttgaag agttcgaagc
     8641 attagcgccg caaaccatct atgtttcggc gacgccgggt aattacgagc tggaaaaatc
     8701 cggcggcgat gtggtggatc aggtggtgcg tccaaccgga ttgcttgacc cgattatcga
     8761 agtgcggccg gtggcgacac aggttgatga tcttctttcg gagattcgtc agcgagcggc
     8821 aattaacgaa cgcgtactgg tcaccacact gaccaagcgg atggcggaag atcttaccga
     8881 atatctcgaa gaacatggcg agcgcgtgcg ttatcttcat tcagatatcg acaccgtcga
     8941 acgtatggag attatccgcg acttgcgtct gggtgagttc gacgtgctgg tagggatcaa
     9001 cttactgcgc gaaggtctgg atatgccgga agtgtcgctg gtggcgatcc tcgacgctga
     9061 caaagaaggc ttcctgcgtt ccgaacgttc gttgatccag actattggtc gtgcggcacg
     9121 taacgttaac ggtaaagcga ttctctacgg cgataagatc accccatcaa tggcgaaagc
     9181 gattggcgaa accgaacgtc gccgtgagaa acagcagaag tacaacgagg aacacggaat
     9241 tacgccgcaa ggcttgaaca agaaagtggt cgatatcctg gcgctggggc agaacattgc
     9301 caaaaccaaa gcgaagggca gaggaaaatc gcgcccgatt gttgagccgg ataatgtgcc
     9361 gatggatatg tcgcctaaag cgttgcaaca gaaaatccat gagctggaag ggttaatgat
     9421 gcaacacgcg cagaatctgg agttcgaaga agcggcacaa attcgtgacc agttgcatca
     9481 gctgcgtgag ctgtttatcg cggcttcgtg acaggataac gaagaagaca gatgacaaaa
     9541 gcaaaattgc ctgatgtgct acgcttatca ggcctacatg atctctgcaa tacattgaat
     9601 tagcgcggtt tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgcgcac
     9661 tttgtcttca ataggaagcc ggaatttttc ttcggatttc cgttaaccta aagcctgtaa
     9721 cgccttttcc agcgcgttat gtaacaactg gcggtcatga cgatacggaa tatcgctggc
     9781 ctccagtacc tcctgaatca caatccgctc tttcaccgcc gagacatcca cttttggccc
     9841 gacgatgacc gcatcaatga cctttttacc aacatactgc tccataattg ccagcttgct
     9901 ttccagcttc aaattagccg caggtaaact caactcacgc cccagattgc cgatatagac
     9961 catcggcgct ggcgtgcggc gtaatgcctg ggcgatttcc ttcagcagca gaattggcat
    10021 caggctggta taaaaactgc caggcccaat aatgatgaga tccgcttcat tgatagcgtg
    10081 aaccgcctca cgcgttgcgg gtacattcgg cgttaataat aactcttgaa tcggcgtagt
    10141 taactggtcg atattgacct cgccgtaaac ttcatgcccc tgatcgtcaa tcgccatcag
    10201 atcgacggga tgctctgaca ttggaatcaa atgcgtatcc actttcagca gattacgaat
    10261 taaattgatg gcttccagag gccgcacgct aaggtgatcc agcgccttta acatcaagtt
    10321 tccgagatta tgaccggaaa gttcgccatt gccgccaaaa cggtattcaa acatcgcgga
    10381 ggcgacgctc ggttccgtta tcagttggtt gaggcagttg cgcatatcgc cccaggcaat
    10441 gccgccttct gaacggcgaa tacgccccgt cgagccacca ttatccgtgg tggtgacgat
    10501 acccgttaaa cgagaaccca aagacgaaag tgatgagaga acgcgtccca gtccatgccc
    10561 tccgccgaga gcaacgacac gatcaagatc agccagcgta cgattgcgca tatagtttcc
    10621 tggagtcaga ttatccgcgc tacagtagcg caaattgcgg ggaaacagca attaacctgc
    10681 caatattgag gatacaaact attttcttct acctctaaag gacgatgcac gctatgcctc
    10741 cctgatgatg tatatcaaag taaaactgcc attttccctt attctgtagc gaaatagcac
    10801 gatcatgacg ctatatacat gattacatag cgaaagtgtg gatggtaaaa atctcattta
    10861 cacgctagta tcggcttaac cactaaacac tctagcctct gcacctgggt caactgatac
    10921 ggtgctttgg ccgtgacaat gctcgtaaag attgccacca gggcgaagga agaaatgact
    10981 tcgcctcccg tatctggaaa ggtgtacatg gcttcacaac tgactgatgc atttgcgcgt
    11041 aagttttact acttgcgcct gtcgattacc gatgtgtgta actttcgttg cacctactgc
    11101 ctgccggatg gctacaaacc gagcggcgtc accaataaag gctttcttac cgtcgatgaa
    11161 attcgccggg ttacgcgcgc cttcgccagt ctgggcaccg aaaaagtgcg cctgacagga
    11221 ggagagccgt ctttacgccg cgactttacc gatatcatcg ccgctgtgcg ggaaaacgac
    11281 gctatccgcc agattgcggt caccaccaat ggttaccgtc tggaacgcga tgtggcgaac
    11341 tggcgcgatg cgggacttac tggcattaac gtcagtgtcg acagtctgga cgcccgccag
    11401 tttcacgcta ttaccgggca ggataaattc aaccaggtca tggcagggat cgatgctgca
    11461 tttgaggtcg gttttgagaa ggtcaaagtc aataccgtgc tgatgcgtga tgttaatcat
    11521 caccagctcg acacctttct gaactggatc cagcatcgcc ctatccagct gcgtttcatc
    11581 gaactgatgg aaacgggcga gggcagtgag ctcttccgta agcatcacat ctctggtcag
    11641 gttctgcgtg acgagctact gcgtcgcggc tggatccacc aattacgtca acgcagcgac
    11701 ggtcctgcgc aagtcttttg ccatccagat tacgccggag agattggcct tatcatgccg
    11761 tatgaaaaag acttctgcgc cacttgcaac cgcctgcgcg tttcctccat tggtaaactc
    11821 catctctgcc tgtttggtga aggcggcgtt aacctgcgcg atctgctgga agacgatacc
    11881 cagcaacagg cgctggaagc gcgtatttca gcggcgctgc gggagaagaa acagacccat
    11941 ttcctgcatc aaaacaacac cggtattacg caaaacttat cgtacattgg cggctaaaac
    12001 gtcaaaagga gagatcagat gagtcaggta agcactgaat ttatcccgac ccgtattgct
    12061 attcttacgg tttctaatcg tcgcggtgaa gaagacgata cctccggtca ctatttgcgc
    12121 gattcggcgc aagaagcggg ccatcacgtt gtcgataaag ccattgtgaa agaaaaccgc
    12181 tacgctattc gcgctcaggt atctgcgtgg atcgccagcg acgatgtaca agtggtgttg
    12241 attacggggg ggactggcct gacggaaggt gatcaggctc ccgaagcatt gctgccgttg
    12301 ttcgaccgtg aagttgaagg ttttggtgaa gtgttccgga tgttgtcgtt tgaagagatt
    12361 ggcacttcca cgttgcaatc tcgtgcggta gcgggcgtcg ccaacaaaac gctgattttc
    12421 gccatgccgg gttcgaccaa agcgtgccgt accgcatggg aaaatatcat cgcgccgcag
    12481 ctggatgccc gtacgcgtcc gtgtaatttc catccacatt tgaagaaata agtatgtcgc
    12541 aactgaccca tatcaacgcc gctggcgaag cgcacatggt ggatgtctcc gccaaagcgg
    12601 aaaccgtgcg tgaagcgcgg gcggaagcct ttgtcaccat gcgcagcgag acgctggcga
    12661 tgattattga tggtcgccac cacaaaggcg acgtatttgc cactgcgcgt attgtcggta
    12721 ttcaggcggc aaaacgcacc tgggatctga tcccgctgtg tcacccgctg atgctcagca
    12781 aagttgaagt caatttacag gccgagccgg agcacaatcg ggtgcgtata gaaaccttat
    12841 gccgcctgac cgggaaaacc ggtgtcgaaa tggaagcgtt aaccgcggcc tccgtggcgg
    12901 cgctgaccat ttatgacatg tgcaaagcgg tgcaaaaaga tatggtgatt ggtccggtac
    12961 gtttgctggc gaagagcggc ggcaagtcgg gtgactttaa ggtggaagcg gatgattaaa
    13021 gttctttttt tcgcccaggt gcgcgagttg gtgggaacag atgcaaccga agtggctgcg
    13081 gattacccaa ccgttgaagc gttacgccag cacctggctg cgcagagcga tcgctgggcg
    13141 ctggcgctgg aagatggcaa attgctggct gccgtcaacc agacgctggt gagttttgac
    13201 catccgctga ctgacggcga cgaagtagct ttcttcccgc cggtaaccgg aggttaagat
    13261 ggcagaaacc aaaattgttg tcggcccgca gccgttcagc gtaggagaag agtacccgtg
    13321 gctggctgag cgtgacgaag acggtgcagt ggtcacgttt accggtaagg tgcgcaacca
    13381 taacctgggt gacagcgtca aagcattaac cctcgaacac tatccgggga tgactgaaaa
    13441 agcactggca gaaatcgttg atgaagcgcg taaccgctgg ccgctggggc gcgtcacggt
    13501 gattcaccgc atcggggaat tatggccggg cgatgaaatc gtttttgtcg gtgtcaccag
    13561 tgcgcatcgc agcagtgcgt ttgaagccgg gcagtttatt atggattatc tcaaaacccg
    13621 cgcaccgttc tggaagcgcg aagccacgcc ggaaggcgac cgctgggttg aagcccggga
    13681 gagcgatcag caggcggcaa aacgctggta gttttttgtt agccggataa ggcaccgctg
    13741 cgtccggcaa tttcaccgct cagtacatgc gtttacgctg cgtcgatgca cagcctcatc
    13801 attttgcagt atccttaaga tattccttat atcttcagga gatcgtcatg gacagattcc
    13861 cacgttctga ttcaatcgta caaccccggg ctggcttgca aacttatatg gcgcaagtct
    13921 atggctggat gaccgttggc ttgttgctga ccgcatttgt tgcctggtat gcggctaatt
    13981 ccgcggccgt gatggagctg ttgttcacta accgtgtctt tttaatcggt ctgatcatcg
    14041 cgcaattagc attggttatt gtgttatcag cgatgattca aaagctgagc gcaggtgtaa
    14101 cgacgatgct ctttatgctt tattcggcgc tgacgggtct tacgctttcc agtatattca
    14161 ttgtctatac cgctgcttct atcgccagta ctttcgtcgt tactgccggg atgttcggcg
    14221 caatgagcct gtacggttac accacgaagc gcgatttaag tggctttggc aatatgctgt
    14281 ttatggcgtt aatcggcatt gtgctggcat cgctggtcaa cttctggctg aaaagcgaag
    14341 cattgatgtg ggcagttacc tacatcggcg tgattgtctt tgtcggattg acggcgtatg
    14401 acactcagaa attgaaaaat atgggtgagc agattgatac ccgcgacacg tcgaacctgc
    14461 gcaaatattc cattcttggc gcgttaacct tgtatctgga cttcatcaac ctgttcctga
    14521 tgttgttacg gatcttcggc aaccgccgtt aattcttttt caccatcact atcccggcca
    14581 tgtgtcggga tttttttctt actaaaaacg acacttaaca agtaccaggt ataaaatatg
    14641 ccttatgagc gagcggaatc gttttccgta tccataaata caaaagtatt ttgtatgcgt
    14701 ggttttaaat ttattgatgg tgaatattaa tattttttct aaggacaact aaaagaatgg
    14761 aatcatactc gcaaaacagc aataaattag attttcagca cgaggccagg atataaaacg
    14821 gtatatggct cattaccgct ttaggtttgg tggcaaccgc aggactagcc tggggagcta
    14881 agtatctcga aattacggca accaaatatg attcaccacc aatgtatgtc gctatagggt
    14941 tattattact ttgtatgtat ggcttaagta aggatatcaa caagataaat gccgccatcg
    15001 cgggcgtaat atatctgttt ttactctctt tggtggcgat tgtcgttgca agtttagttc
    15061 ctgtatatgc cattatcatc gtgttcagca ctgcgggcgc gatgttttta atcagtatgc
    15121 tggccggttt attatttaat gttgagcctg gttctcaccg ttttatcatt atgatgacgt
    15181 tgacagggtt ggccctggta atcatcgtga atgcggcatt aatgagtgaa cggcccattt
    15241 gggtaataag ttgcttaatg attgtgttat ggccaggcat tatctcgcat ggacgaaata
    15301 agctccttga actggcagga aaatgccata gtgaagagtt gtggagtccg gttcgttgcg
    15361 cttttacagg tgcattaaca ctctattact attttatcgg cttctttggg atacttgccg
    15421 cgatagctat aacgcttgtc tggcaaaggc atacgcgttt ttttcattag ccgtaaatgc
    15481 acatactaaa gaacttaact aaacttcaca tcgccgcttc atttttcgcc cgtagcccag
    15541 tacaaagaaa tagttcacgc tttgaccaag taacagccag ataatcgccc ccattaccat
    15601 ccagttaacg ctggaaatca gcatctgagc gagggcgaat ttccatgaag gcagcaccag
    15661 cttttgccct ttgatggtca tatggcggtg cttcgcgaaa gcgcaaaacc acaaataaac
    15721 cgcgataatc atcagtaagc caatgccgag aatacgcagc gtagtttgat cgacatacca
    15781 gtgatccggc aactccacca cgcctgcggt aaagataatc cctgccagta aaatatagcc
    15841 cagccagttg gtggtaatac tgagcgagaa aatccgcgta atagtgctac cgggcaaccc
    15901 cagacgagag tacaaacgat agcgcatacc aatgccgccg acccaggtac tgagcgtcag
    15961 gttgaaggcg tagcagataa acgacaccag catcacctgg cgcttcgcca gtttgtgacc
    16021 gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc agatagctga cgaccaccag
    16081 cgaccgcact aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc
    16141 agtccacttt tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg
    16201 tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc
    16261 cgtgttttca gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac
    16321 ctgcgccaga cgtggcgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa
    16381 aaagtggaac gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt
    16441 ttcatccacc tgctgacaat ctgcggcgat aatgccgttc agattatcgc gcagcgtctg
    16501 gttaaaatta cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg
    16561 atcgagatta ctggacccta ctgtcgccca gtgatcgtcc atcaatgcta ctttgccgtg
    16621 gagcgggcgg cggcggtact caaacacctg aacgccgcct ttaaccagat agttatacag
    16681 caagcgcgcg ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat
    16741 ccgcaccccg cggcgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa
    16801 gtaggcgttg gcgataatga cttcccgccg cgcctgagtg agcattttca gatagtggcg
    16861 ttctatatca tcgcggtgtt cttcgttatc gcgccagacc agcaatacct gcgcgtctcc
    16921 cggctggcgg ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg
    16981 tccaggcagg ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg
    17041 taccgcgtaa tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt
    17101 cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg
    17161 aaacacattg gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac
    17221 gccagctgcc gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc
    17281 cagcaagact tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg
    17341 tttgccgacg ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc
    17401 gccaatcgcc ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt
    17461 attgccttcg cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca
    17521 gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg
    17581 acgtagatcc tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt
    17641 ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct
    17701 ttttgccgcc agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca
    17761 ttcacccatt cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga
    17821 tgtacgcaca tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc
    17881 acgccgcgct tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga
    17941 taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag
    18001 gcaaaatcgc tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt
    18061 tccacatgca gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg
    18121 gcgctgacgg tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg
    18181 gtaaagcctt tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg
    18241 ggcataattt tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa
    18301 aaggtgcggt cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat
    18361 tcttcaggag agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt
    18421 gctggcagat tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta
    18481 agactctggt tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga
    18541 tgctggtgtt tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag
    18601 agctgaccac gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg
    18661 tactggaaaa caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg
    18721 tagtgtcttt gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac
    18781 gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata
    18841 tccagactcg catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg
    18901 ttaatccacg tcaggttttg cagccatact ggcatgtttt ccaccggaga aacgtaaccg
    18961 gaaaggagaa tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc
    19021 gagcagagtg atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc
    19081 gtaaagtaga acagcgccag cgatccggcg aaggggattt gatacgccca gataccaatc
    19141 gccagcacaa tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg
    19201 aagatctgcc aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt
    19261 tcgcgggcga cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc
    19321 agcgacggca ccacaaacca tttgtagtca agattcgggt tataccagtt gcgtaccacc
    19381 agctcgctgt tgttaggttt cggttttcct tccagcagct cctgctgata atttttgacg
    19441 atctgttgca ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc
    19501 aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc
    19561 accagtagta acgccttttg tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc
    19621 agcagcacat gggtaaaggc gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc
    19681 tcgccgttat cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg
    19741 aacgggaaca ggatcacctg aataagcaca ggtaaaatca gaatcgcgcg ggtttgtggt
    19801 tcgcgcagca acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc
    19861 ttctccctaa tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc
    19921 cgaagcgatc aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa
    19981 caggctttgc agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat
    20041 caccgcgggc atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcaggaa
    20101 cgcggcgttc agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag
    20161 cagccccatc cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaataagcag
    20221 cgaaccgcga tacggcacgc cgagaataaa cactgacacc agcatacaca gcagcatcgc
    20281 cagcatcccg agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat
    20341 ctccgtagag agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga
    20401 ggtgagaatc gcgccgatga ccgtcatgat gatggtcact gcaccgggga taatgaagtg
    20461 ctggctaatc gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa
    20521 agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgtg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 ccggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaaggccg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgt tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 cc
//
LOCUS       DAZPAC010000018        34504 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZPAC010000018 DAZPAC010000000
VERSION     DAZPAC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.018"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(4..234)
                     /locus_tag="ACHUPM_000734"
     CDS             complement(4..234)
                     /locus_tag="ACHUPM_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868602.1"
                     /translation="MFVLIAGVNVHNEYYVNRIAGIAGYAGRAVELIDETTRKIDLLS
                     DQERKKADVNDADIFLMLKAFVEMGFKISLHK"
     gene            complement(256..459)
                     /locus_tag="ACHUPM_000735"
     CDS             complement(256..459)
                     /locus_tag="ACHUPM_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868603.1"
                     /translation="MVSYDKIRAEYRAKYRAYKLELIDDLIAQRDQLNFTFSDLLNSK
                     RDCKRKREYLRLSALIGKLQNSI"
     gene            complement(508..1158)
                     /locus_tag="ACHUPM_000736"
     CDS             complement(508..1158)
                     /locus_tag="ACHUPM_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868604.1"
                     /translation="MANIIISKKSIIEAASIVSDELREKADLATQTYNEHYKNGTHTK
                     ADKANMQAATTKLAYFINNVVNAVEDEKLCSVFYYAIKASKQAPEVFFRDAMTNSYSL
                     EKLVYLVKSIKSGKCVYSIADMSGSRVFALIDMINDEIDTFTNGAVFDLMNEAKKACE
                     IKLDAGYTQANQLINLCERLGLVEKVKGAGIAKAGTQQYRFIKNDFYNYLADAFKA"
     gene            complement(1447..1971)
                     /locus_tag="ACHUPM_000737"
     CDS             complement(1447..1971)
                     /locus_tag="ACHUPM_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0868605.1"
                     /translation="MRTLDITYGIPAEVWPRDYEHAERTLLFWRKAKFPVRVTLEDGQ
                     VFCMYVQGTMSSRNKLDLCPAPLDNDNRIRLPLERISTIESGVIDGIAYDFKGRLTIH
                     PDYVDNQPSRRDFFKICRQAYQNGKSIRVYMADGREIEGTSDGVDACLVTLKLEDGRK
                     ILIFFDWVERILPF"
     gene            complement(1968..2561)
                     /locus_tag="ACHUPM_000738"
     CDS             complement(1968..2561)
                     /locus_tag="ACHUPM_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868606.1"
                     /translation="MKLSLLLACIYLPAFSANALSDFVSDMQKTYSMFTSEDVVEYKS
                     RGLNQPVWFRFSDNAEETILNGGKERLRLQINGTAVPENVNVSSDEAMKPSGIVLEVQ
                     DGTLYCDEMATTGCQVNIRVGNEDPIVTYAKAEGNTSGKVLFIDENTSKRIVRDIQKT
                     ANSKSVDKKIYVEVPFFVVGNKEFKFGLLKTPLRVSE"
     gene            complement(2583..2903)
                     /locus_tag="ACHUPM_000739"
     CDS             complement(2583..2903)
                     /locus_tag="ACHUPM_000739"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868607.1"
                     /translation="MKKISSEEYFISHIYIYPFKLEPLNIILPTIKGVELEGYTLSSN
                     FNDMGVQKPGTRSYYAYKVKCNNYNALNMLISKLETEIVEGNLNDDHEKLEQIERLRK
                     CDVK"
     gene            complement(3217..3783)
                     /locus_tag="ACHUPM_000740"
     CDS             complement(3217..3783)
                     /locus_tag="ACHUPM_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868608.1"
                     /translation="MSWLKELSFVDTTGAKKNHSFWKVSDPSDFLVGANAAFELLNFY
                     QRYPEMKHSPLLYRITNDMNRSGLLSSESAKGFFSKITPHISFDAANEIDYLSTKPQE
                     QLSVPPEKKERRIASIENISKELRTTTLRLLNFLTLLNWIDDQTLIPSTEALDNRVLR
                     LNKKSQFGFVFTKKGEDLIKRKYRLLNN"
     gene            complement(3849..4127)
                     /locus_tag="ACHUPM_000741"
     CDS             complement(3849..4127)
                     /locus_tag="ACHUPM_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868609.1"
                     /translation="MSEKKTTYCQVALSDKANEKLGKFQIKLKEKKIKMSKAEVINAI
                     LETMTMAEFDKVTSAVGVSAKTREKIMRIYENSNMTKKDLEEILSRLP"
     gene            complement(4130..5689)
                     /locus_tag="ACHUPM_000742"
     CDS             complement(4130..5689)
                     /locus_tag="ACHUPM_000742"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR0868610.1"
                     /translation="MTVRIVSNAVNALISGADDKVKQLVQQMLSYEVEAGDWKGTSTM
                     FNWSKNSFPAGFAKPVAANLNKAGIKCVHIRKDKAPALGKPNPAVNPFPYNPDYAYQD
                     QTVETLVREGMMIAQIATGGGKSNVACKAAARIGRMTLFLTTRSVLMFQMAENFQRSI
                     DYRAENGEPWLKGQKVGVIGSGEFQVSRHINVATVQTLASFLEEPPRDATPDKKSYHL
                     KRRELVKRFLSSVSLLILEEAHESSGSNFYDIARLCVNADYRLALTATPFMKDSTEAN
                     MRLMAMAGRIEIKVTEKYLIDRGILAKPYFLYHKVAYKPDEARIKAELANKHLNFRVG
                     MSTAYQKAYQLGIVYNLGRNEAIVREALLYKQHSLNCMTLVRLKRHGQILMEMMKESG
                     LIVDFIYGESNQATRQAKLNSLASGEIDVLIGSTILDVGVDVPSVGAVILAGGGKAEV
                     EMRQRVGRGLRAKKNQANVCFITDFIDISNKYLLSHSYERKHIIDTTPGFAEGVLPVD
                     GAFDFGVLKRD"
     gene            5772..6452
                     /locus_tag="ACHUPM_000743"
     CDS             5772..6452
                     /locus_tag="ACHUPM_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR0868611.1"
                     /translation="MDVKITILQVDVANLRPNTWNTNSVGAQNFEKLKGSIEKLGFFK
                     PILARELEDGFFEILGGEHRWRAAIEQGISTVPVLSVGKISDVVAKQMSLVDNERYGE
                     DDQIALQRFIEEIQSELDYQLSEIAPYDDEISMVLAKEAAIDLEALEALSRGSDEPVD
                     TDKREKTERVGAEHQTMRFKVTFDASDRVAETIKNIIKDQAINTGNEMENAGEALVWL
                     VDYYKERM"
     gene            6452..7120
                     /locus_tag="ACHUPM_000744"
     CDS             6452..7120
                     /locus_tag="ACHUPM_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HFR0868612.1"
                     /translation="MTKKFEIVYRDPADLIPYEMNAKKHDEQQIRDLAAAIKKRGFDQ
                     PITVDKNDVIITGHGRREAAIFAGLERVPVIVRDDLSDDEVRAKRLEDNRLASIDYDA
                     IKLQKELESLVLDDIEVFGFEERELNVLVGSMTEEMDTDSLVIDLSEETKRQKDEHTE
                     ISREVAAEEVRVVDVLGFKTLPAGSAIVVGDLLAHMEEMTGESGVDAFVAYAEKISSG
                     EMAA"
     gene            7117..7782
                     /locus_tag="ACHUPM_000745"
     CDS             7117..7782
                     /locus_tag="ACHUPM_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0868613.1"
                     /translation="MSKYNINVSFQTRVNKTTRTLEIAESFGLGLDEKEWTLYDNLEL
                     EVKQGDVVYITGQSGSGKSVVLRELQRQMKDEGLSVASIDDFTFDNEVNVIDQLGKTT
                     SDALGLLSMAGLNDAYLFVRKPSEMSDGQKYRLKIAKLIESGAKVWAADEFGAVLDRV
                     TAQVVASNLQRAARKVGATVMVATTHEDLKNALRPDMQITKHYKERVKVEYHNGSHDE
                     VHL"
     gene            7779..8669
                     /locus_tag="ACHUPM_000746"
     CDS             7779..8669
                     /locus_tag="ACHUPM_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868614.1"
                     /translation="MTDIIIKRYRPEEFPRHLDFLERMTVTKGTVEDWHALKSLHYKT
                     DGKPFAPTYYRCELDDRLVGVVVMAYPKLLLAPRHRMFPKLKPTTNTTVANQYWGRYV
                     NNNFAVISRSVVDTQYRGVGVSYRMINLVSRMHDRPIIEIQSSMSKYNPFAMKAGFKF
                     IRPERPKSYESALRVFQRHFRSDTGDNEAIVKELFAMSESRRRRALRDLVADYHKNSS
                     LAKAGRNRGTTIQDIADSLVDEASIVKLLKDIHNLSFTSPLYGVYRNPDFGRRLPDTL
                     PLLAFDKQPLDKPLEIALPA"
     gene            8679..8945
                     /locus_tag="ACHUPM_000747"
     CDS             8679..8945
                     /locus_tag="ACHUPM_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868615.1"
                     /translation="MTLTDKQKDIIKTLNLGYERGHLLDLDELLEVLPYKTTKQSIQF
                     SIRALIKKGLVEKGHTRQRSDNRYHRRTLGLTTLGRAKAKLLVM"
     gene            9141..9773
                     /locus_tag="ACHUPM_000748"
     CDS             9141..9773
                     /locus_tag="ACHUPM_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0868616.1"
                     /translation="MTVEKDEVKTRLTPAEWAEAEAKWTSGEYTLSKLEEEYGIRRET
                     LSRHFKKRGLEKGADSVGKMVRESLKSDAELRAKARAEKIEERRTRYDGWAYALGQMV
                     MVEVTTAKREGKPLGAIEDSLKSLQRASNTLAKCFEVSSKALGMDHAENDEEEIPNLV
                     FGELTPSQVAKLRQEDDEPEIIDDELLETLEEEALSEFDATDDGSEGEDK"
     gene            9773..11029
                     /locus_tag="ACHUPM_000749"
     CDS             9773..11029
                     /locus_tag="ACHUPM_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFR0868617.1"
                     /translation="MAIPSSLSLVQLHSGQMKVFQSPHRFKVVCAGRRWGKSRLSIST
                     IIRAAAKEKKQRVWYVAPTYQMARQILWDDLQEVLPRKWVRKKNDTTMTIVLKNGSEI
                     ALKGADKPDTLRGVALHFVVLDEFQDMKADTWYKVLRPTLSSTRGGALIIGTPKGFSE
                     FHKLWTIGQNVELQRKGQWKSWQFVTADSPFVPTAEIEAAKNDMDPKSFAQEYLASFE
                     NMSGRVYYPFDRNVHVKPLQFNPRLPIWVGQDFNIDPMSSVILQPQPNGELWAIDELV
                     LFSSNTAEVCDELERRFWRWKSQVTVFPDPAGAYRQHARGESDVDIFKEKGFLRVDYS
                     KKHPPIADRVNAVNRMLMSASGDIRLYIDPKCKHLIDSLEKVIYKPGTRDMDKTGGIE
                     HSADALGYPVHRRYPVKNRVILGGSR"
     gene            11056..12630
                     /locus_tag="ACHUPM_000750"
     CDS             11056..12630
                     /locus_tag="ACHUPM_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR0868618.1"
                     /translation="MDLNDKQIKDLVARRHPEYEKKKEHWDFLASTYAGGRGWFTDNI
                     FRYFKEGDQEFKERVERAYRFNHTREVVNLINKYLFKEDIHRNIEEAPEQIRNFWKRA
                     TRQNASIDSFMAAIDLQSSIYGRIWVVVDSTMSGDVESVADEKKKDARAYAYWISPQQ
                     MLDVAWDDDGNMLWALIVEVARDDADPFTSTGQEYQRYRLWTQNEWYLFREEVKKGAG
                     GAGRRQAKVILEDSGEHNLGVVPVFPVDCIGESESPYFSPSLIDDIAYLDRAVANYLS
                     NLDAIIQDQTFSQLAIPVQSLLPGDENHTKVLEMGTKRVFTYDSEGGNQPFYLSPDPK
                     QAQMIITTIKTVINEIYHSVGVAGERTKQDNAQGIDNSSGAAKMYDFQRVNSLLVTKA
                     ERLERAERQIMLLVAKWMGVDLDEDHSLIAYPESFDIRGLTDEFSVAEKLSLLQAPDS
                     VRRHQMEMLIEKIFPNITEAMKKEFDKDLLNFPPKNDLNTLENKSVLTYDRGAAQESG
                     QDQPRGNGDSSTQENE"
     gene            12652..13539
                     /locus_tag="ACHUPM_000751"
     CDS             12652..13539
                     /locus_tag="ACHUPM_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6651 domain-containing protein"
                     /protein_id="HFR0868619.1"
                     /translation="MNLWQMLLARRGLMDVAEAHERGGAGGVAADNEQSTQDPDKQGE
                     QKEQPKGDDEYAGMTQEELLAELRKTKKAGAELLKENMKRKEKERTLADQLAQYGDID
                     PARARQLLEAEQAAENARREAEQAELERRGEFDAVKKQMIEAHQAELAQRDERYTALE
                     SENASLKSQLVEMTVGASFSNSLFLRDKVLMTPAKARVIYGSHFEVGEDGSVVGYDKP
                     AGHKERAVLVDGEGKPLPFESAIERIVRADPEADALMRSEAKQGVGSHSKSTYTISQP
                     KNKSTMDKLTSGLGKIGLK"
     gene            13565..14485
                     /locus_tag="ACHUPM_000752"
     CDS             13565..14485
                     /locus_tag="ACHUPM_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR0868620.1"
                     /translation="MPLLREEAEKLSNNELEQGVIETIIDRDDLFAILPFMKINSKAY
                     LYNREATLSEATFIDVNDTIAEGAATFSEHVAKLRILAGDVDVDKFLATTMSDTNNQL
                     AVQVRQKVKGLARAFRRNLILGNSETNTKAFDGIPRLMHKDQKIDIAGASMTFSMFDE
                     LVDAVKDLGADCIMMRSEHLRAYRALLRTVNVGPSEIMMENFGRPMLCHNGIPFIVND
                     FIPKNGVKVTEGSEQKTEGQEGTANIYCLHLSEENGLTGLYGGDNAGIVVENIGTVQN
                     KDAVRTRVKWYCSLANKHDKAIAALNNVKI"
     gene            14583..15017
                     /locus_tag="ACHUPM_000753"
     CDS             14583..15017
                     /locus_tag="ACHUPM_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868621.1"
                     /translation="MKPAKIRLLEPQFSDYSGMLCGVKFENGVSVSELPFIDQQRICA
                     SMRASTVEGKNVSPSAAYGERNDLNVDQIVEPSAPDIVPMKRGTADEPAKPIQTFTRE
                     ELESIADSEGIAGLRSIGNKIGVKAKGIVEMIDGILKAQGGE"
     gene            15017..15850
                     /locus_tag="ACHUPM_000754"
     CDS             15017..15850
                     /locus_tag="ACHUPM_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868622.1"
                     /translation="MAQIDSYRSGEAVSLSFAFNVLDIESATYTVKDSTGAILVDGEP
                     LEITSGQMSIPVVVSAEYNQLSEKERDLRYVIVKAVASGLTHEERQMYVLLNTFELSI
                     PEQSFATVADAQMQAIDMLNGDTLLSDGEGLMRKRLIEATRRIKTLPFSIRKILRIDF
                     DRYDRPQNMLNVYDIPWGADGAYRHDLVDWEKITQEKFEEFPDYFKEALMLAVVNEAC
                     EIANGNDIVAAREDGILSESIGETTNMYRTGKSANVHVARSTWRLLIRYIDNRMIVRR
                     S"
     gene            15922..16266
                     /locus_tag="ACHUPM_000755"
     CDS             15922..16266
                     /locus_tag="ACHUPM_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868623.1"
                     /translation="MNISWQTEIAIYRFGTKNVYGEAQLQFVRKTNVGVVKFEQSNEK
                     SSVRADSSGSRGKASLELFDAVLVVPLESAVELDDVLILEGQKLKVSSVHRRWGLRGR
                     PGHLEVGANIWV"
     gene            16257..16730
                     /locus_tag="ACHUPM_000756"
     CDS             16257..16730
                     /locus_tag="ACHUPM_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868624.1"
                     /translation="MGLKYDAHQFKRAGNRLNNSQKAFKRYLIRDMEKLARLVERLSR
                     AMAPLETGSLETAIFARVIKEGYSGLRIELSVSGAKPRQGHPGVEVGDYAKYMELGKY
                     RLGYLSRMKNVTNPPIEGVKPRVGPHFLERAVEISEKQFSEAILEAAKKAGFTRG"
     gene            16732..17115
                     /locus_tag="ACHUPM_000757"
     CDS             16732..17115
                     /locus_tag="ACHUPM_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFR0868625.1"
                     /translation="MFIEAFANLMQKAKIGTVGTDIFCHYLPAYVKSGVLLINPNTGI
                     SIDHELQGFYHESFTIIVRGSSITTTIEKARKIIDMFPVEETESGEVYFRLVRPMAMP
                     VVYPKNDGSLIEAGIPVEFAGYLLN"
     gene            17189..17926
                     /locus_tag="ACHUPM_000758"
     CDS             17189..17926
                     /locus_tag="ACHUPM_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFR0868626.1"
                     /translation="MSNTHVKNIKLGACKVSFGGVDLGYTKGGVQVEVATETLKVTVD
                     QQGQTVISELVQGRNITITAPLAESVLKNMVDLMPGSTLSEDDNSVTITSAQGVNLIN
                     VAKELVLTPQDTTDYVLTLPKAATAGNFTMSYQSDDVRVFSVQFNAYPDDEGVLGKMS
                     GPKPVKNVTITPESPSVKVGATVQLTANFTPDDVVDKTGVWSSDNKKATVDQTGLVRG
                     VEQGSANISFTSNSGSKKVTKLVTVNQ"
     gene            17994..18311
                     /locus_tag="ACHUPM_000759"
     CDS             17994..18311
                     /locus_tag="ACHUPM_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868627.1"
                     /translation="MTKLLDLDSILPPKKSVKFGGQEYPIVEMTVGLFVSIKQMEGKD
                     LTNMSPVEQVTAYADLVRKVIPSVPDEVLEKLTVPQLQQIFTFAMEVIDEENEKAAGE
                     GAK"
     gene            18437..18661
                     /locus_tag="ACHUPM_000760"
     CDS             18437..18661
                     /locus_tag="ACHUPM_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868628.1"
                     /translation="MLSRNIDRLRAEEDVRNLQVALAAQADGESVKAFMEGLQLRIGR
                     PVVTDKVYDPSQDRADPDAKEQLMQIFGRG"
     gene            18669..23231
                     /locus_tag="ACHUPM_000761"
     CDS             18669..23231
                     /locus_tag="ACHUPM_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR0868629.1"
                     /translation="MSQNVEFILSLEDKQFTASIDRAGKLLTRFGEQVTKPAQKIQTF
                     ERSLGSVARIIGVLESKLDSTADKLQDVAAGFELASDATRKMRGNITSLNSGLKALIE
                     RVDTTTSSVDKLTASLRKVQSELNDFSDWATYASKSASRFGTEVKEASASVSGMNTRL
                     NTTTKRLSNWGVTTSQAAEGLKKVREQMDEVIGRQQLINKPVRVRTSGSGNGGGSGGR
                     GGYSWHIGKNNEGSMFSGLRGNIFLLGEIGDAARTVTSIMFGWQKPIIEAAAEMERMR
                     VMLRGLNKEKSNPGQAAADDMKYIVDMAQNAPFAMQALTDSFVKFRSAGLDPTDGSLK
                     ALVDSVARFGGNSELLKRAAVAVQQMSGKGVVSMEELRQQLGEAVPNAMQAMADAAGI
                     TMGELTKAVASGTVEAKQALSLMFVGLRAENENAAKDMMQTYTGALAQLQTSFTLFAD
                     RVGQAGYLDSLSKGMKELASIMNSAEGISFANSLGSGLTTAIDGLRQLAQWLAKNQEL
                     VINLGKVVAAMVAFKLMRAGIMGVVGAGSQMVSTFATMATAIQTPFNLGATAVTRFNR
                     AARMGLAPIPSLIFAIRGAITGLKGAFAGLTAFIAANPIGAAFTVATVAVAGLITYMT
                     MLRSETSKVVDEIRKIPEAMTAAKRAQMAEYKARLEQQITQKEQELKSGEKVVYGPGM
                     AGTTVKINQDERKRIENELNDLRKKRDKTTGAIERGDIAVAKRLAKDAAESQIEKIRE
                     ENRDFAATFVKARQEALDKIQKINDDGSLSDDEKNKLLAPLRETVNKSYLEPAQKLVD
                     SLSSRKNAVEGQIATLNDQLEKAKKEGNTEQIKKLQGSIRGYQENLEVVAQELTQAEF
                     ERDNAAKTGKGVMSNQGTVLGLGTTDKAAQKALAQYMRNQMDSATYQRTLPDGTPMMD
                     FEGKPIIGPKQLKTQLNLQKASSASSLEKMSDEERAAAIAALTKAREQDAAAAEKAAQ
                     RSANASQRAAKKEQAAQQKLAAGYQKALDKADQLMGQMGESSKATVSFDQSLRDTTKS
                     LTDLANAVPNEFITQEMIDKAKKRLEDLKNATPEYREMFNRRNVEQMISTWAPESDSI
                     ISAGYTPSREEKVADFEDTYNRNLKALIELRDKASDPKVVALYTKKINQLIAAGNTAL
                     IKETGTATQKLALEYENLAEQIESTWTDLFSGLTDVLTDFVINGKMSFSSLSQSILKD
                     ITNMVVKSQITLPLMNMLGMGTTAAGSSQSGNLLTGVASAVANQGVRMGNTVNGDKSV
                     GEATKETSSSVTGLGQTTQQTTSAIGTATNAIGSWVSGLFDSTEAKDAETKAVKDSIF
                     SMQNLSSVTGALSAAFAMLGANASGSGNKWLSFGATVASGLVSAWAGGGFDSVTSSSA
                     KTATSSVADGTKGIPAIPKFANGGIFGKDGVIPLRAYQKGGIANSPQLALFGEGDMNE
                     AYVPLPDGRTIPVTLSTDGMSGGGNVLSPVSIEINVHSDGSTTESGDTESIWNNAAQR
                     MKAIALETIAQEKRPGGSLNPNTQRN"
     gene            23274..23609
                     /locus_tag="ACHUPM_000762"
     CDS             23274..23609
                     /locus_tag="ACHUPM_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0868630.1"
                     /translation="MERQTFNWYPDYESEKSVKPNVTVLNFGDDYEQRQAQGLNRIKE
                     EWSLTFTRSYNEINAIDDFLTERSGVESFYWVNPRGKQIVVVCDSHTVKRYQGYCVLT
                     ATFRQVFEV"
     gene            23692..24390
                     /locus_tag="ACHUPM_000763"
     CDS             23692..24390
                     /locus_tag="ACHUPM_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR0868631.1"
                     /translation="MGIKADIQSLSPSALIELFVLDMSNTTSGGKLFFHAGTNELMQP
                     VVWQGVTYEPWPIKASGFDKTGQGTLPRPKIQVSNFAGTVSAEVQANDDLVGCRIIRK
                     MTLARFLDAVNFKDGNPTADPNQHFPDEMWFIEQKTLETHQVVEFELSSVFDLMGVQL
                     PYRQIIKNTCPWKYRGPECGYTGPYFDKNNQQTSMSGADYCTKRYDACNARRNYFADG
                     VIHFGGFIGATRYG"
     gene            24383..25180
                     /locus_tag="ACHUPM_000764"
     CDS             24383..25180
                     /locus_tag="ACHUPM_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0868632.1"
                     /translation="MGNKAIPELGSDVMQQIYLCAINRYPNEACGFLVRTNGDKYRFM
                     EARNVSENPQNTFVMHVDDIMAAEDAGDVIAIWHSHTDESAEASDADRAGCEATEVPW
                     MILAIRKNVEGDAPFHFSEMNVITPDGFEMPYLGRPYVFGVFDCWMLCRDYLKREFNV
                     ELNPNAHLHIPSWYTGDNDILDQNYRNEGLVRLAPGTEPQRGDVFFIQYGKMPDHCAV
                     YIGDGMILHHQIDRLSCRAYYGGMYQKHTTHHLRHRDLLKGDEACLS"
     gene            25168..25758
                     /locus_tag="ACHUPM_000765"
     CDS             25168..25758
                     /locus_tag="ACHUPM_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR0868633.1"
                     /translation="MSELVHVQLGGPMAKHFGRHWHLKVRNTKQALDLIEANKPGFKA
                     WMKRNIKTYDRYHIQITNKQGHKWSVDESEYQMMGQSDNIAKIRITPVPRGSGGSAFG
                     WFQTVVGAVLMVASIWVPALAPLGLSLMMGGIAQIISPQATNESVRQADNSNSFYFDG
                     PQNTTNQGNPVQLIYGEEILVGSQVVSSSITIDQLM"
     gene            25775..30481
                     /locus_tag="ACHUPM_000766"
     CDS             25775..30481
                     /locus_tag="ACHUPM_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0868634.1"
                     /translation="MEQFKKKKLPLLIAGSGGKKSSKSSSRTPVEADDTVNSRAMASI
                     LDLLGEGVIGGLVNGARSIFVDDLPIVNEDGSSNFSGISWDFRDGSQDQTPMSGFDFV
                     ETPKSINIQLKKSHYVTVSIDNDEADRVRVIMKFPSLRRIDQKTGDTNGTTVEYKFQI
                     SNGDSTFVDVVAEGEKNVGIKLTAKKTGAYYRSYELKLPKPGRAYSVRVVRITDDNSG
                     QYLYNDTWVDSIGEIVDTPMNYPNSVLVGLKVNSEQFGGSMPSRSYLVRGLKIRVPSN
                     YNEASNTYDGVWDGSFKLLSSSNPAWILFDLLTNSRYGLGQYVSESMIDLGQLYQIGR
                     YCDEEVDDGFGGKEKRFAINTQITSRQDAYRLIQDIAGAFRGMVFWAGGMVNIMQDSP
                     SDPVMMFTNSNVKDGLFTYKGSARKDRPSVALVTYNNKEDGYKQNIEYVEDQDAMRRY
                     GERKTEVVAFGCTSRGQAHRVGLWLLYTARMESDVITFTAGLDASFLMPGETVLIQNK
                     YRAGKRNSGRIVEFTKNSITLDAPVSLAKSGSFIRILNQEGKIVERDVLETGENITKV
                     TFSKALSSAETPVLNGVWTITEPDLEPMRVRIVNIAQGETSGSFDITAVENNPSKYEA
                     IDNGATLIPQNTTVLDPTYSKPSNLQITEGTYLSSPGNLSVKLTATWEGKSPEYWISW
                     RRSDENNVSNWQSARVTEEQYEIVNVAENGRYDFQLYAVSFNGKKTEIISTVYQVLGT
                     MTPPDAPTSLTAVGDYRNVILNWVNPDSVDLDHINVYASQTNNLDTAKLIAESASTTF
                     THAGLGDSETWYYWVRSANKRGMLSRPNSNLGTEATTRDVLSFLANKITSSELGQALN
                     EEIDSKASQQAVDEINNHIDQSVESLEGEISALNKELTSSIDEFKTSFTERSETLENA
                     QSELRTEVSSTREKVDDALRQVENSNAALIELRNTVSKQGDSVAGVVEAANAALENAS
                     SLIAEERKVRAQADLANAKKIETMRSSVDESVAAVEEMRRTVAEVGRVSSEITTNLEA
                     ISKTNIDLALRQDEDQHRQLVNNAKIATTQKILVDDVSAMASKVEEIRAEIGDNIRAS
                     ILEEATARTDGDNALANRVTQLQSKFEGDISAAIRTEQSARASANETLSKQITQLESK
                     VNTDISTAIREEQQTRATQDEALSRQITQLQTKVNNDISAAIKSEQQARVSGDSALST
                     QINQLQAKVDDDISAAINEEREARASGDSALSREVSSLKTKTGKDIAAAVAVETKART
                     DANDALSRQISSLTTRANNLQSSIERESTSRTSQDSAIAKDLASFKTKTAKDISAAVA
                     VETQARAAADTALTSQINSLKTQTGKDIKAAVAEETKARTDADAALSTRVTNLQSQTT
                     NYINAAITSESKARTSADNALGKRIDTLKASVDGNVAVIQQQASAIADANRKVSTSYT
                     LKMETSTSGGQKYVAGIALGIDTTGLSQFLVRADRFGLVNNVNGKITTPFVIENSVAY
                     MNGAYIKDGTITNAKVGDLQSTNFVRGRAGWRFGKNGTLEINGNSGGNGRLVINGQRI
                     DVYDDNNVLRVRIGRL"
     gene            30576..33947
                     /locus_tag="ACHUPM_000767"
     CDS             30576..33947
                     /locus_tag="ACHUPM_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR0868635.1"
                     /translation="MWYREGTITFTQGSNTLVGAGTAWNVTANGVLPGMIVVAPDNKL
                     YEIKSVTNDTTLTLVENYTGETQSDIPCRIITTYEGDLTQFSARFTALLSRMSGDAKT
                     VRNWLTAVDEVTLEREDGTNVNVKPLSKIIAEHNKHVEWYENNTNAINSAGDKAKQAA
                     ASASAAAKSANLASSKATQASNSALDATASKNAANKSASSAKASETSALEAKNAAAIS
                     ASTASTKATEATSSANAALVSKEAAKLSEMNAASGAGSASTSANQARESARSAKTSAN
                     QASTYASAARASKDEASRKSAEASTSASNASSKSAESAAKAEEAKQSQTSALASAERA
                     EIAATRAERALSAISLHDASLTMKGIVKLSSETSSSSEEFAATPKAVKAAFDLAKSKY
                     TANDATTSQKGLVQLSSDTNNASESLAATTKAVKSVKDLADRKAAINSPAFTGTPTAP
                     TATQGTNNTQIATTAYVREAISGLVGSSPEALDTLNELARALGDDPDFATTMTNKLAT
                     KQPLDATLTALAGLTTAANKLPYFTGNDTAGLATITSVGRDILSKASVQAIIQYLGLK
                     ENGTSGDKIPLLSTANTWSAKQTFSNGLAGSLNGNSDTATKLKAARKIGGVPFDGSSD
                     INLPGVNSTGTQSTSGNAASATKLATPRSIGGMLFDGTANIDLPGVNKVGNQSTTGNA
                     GSATRLQTARTIGGVSFNGTANIDLPGVNKAGNQNTSGNAATATKLATARKINGVSFD
                     GSKNISITPKQLGAAKYVANLATGNTQEWTTAQFVAWLNSQGAFDATQWSTRCSWSYG
                     SNCYIGNASTGCGVIPLAGSVIEVFSTGTSNYTIRITTPSTSGGASGAVLNSEFIYTY
                     NGDNYSPTWRRNYSSQNKPTASEIGALASNGNAVSATKLKTARNIGGVSFDGTANIDL
                     PGVNKAGNQNTSGNAATATKLKTSRTIGGVSFDGSANINLPGVNTSGNQNTSGNAASA
                     TKLQTARTINGVSFDGTKNISISAAQIGALPIGGGTITGNLTVNGNGSFNDVQIRSDK
                     RNKRNLVKLDNALDRLEALTGYLYEIQLSDDSWKTSVGLIAQDAQKALSELVTEDTDV
                     ISGEKRLCLNYNGIIALLVEGFKTLRHEIKELREK"
     gene            34033..>34504
                     /locus_tag="ACHUPM_000768"
     CDS             34033..>34504
                     /locus_tag="ACHUPM_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868636.1"
                     /translation="MGVTSGWVGSSAKNETGEQWMSAAGSKLGLGKPFMMSQMVGRSM
                     GCKIATAYYKGNPPDKVENWGAVGSDWPLAEKNQGTIAGASSCGLGRLVGIVLTQAHY
                     VPGLPPTGGLYIAGGRASNITVTVGGVSQVFTYNSVINGFHYYWQGTPSSAFITA"
BASE COUNT         9923 a         7154 c         8476 g         8951 t
ORIGIN      
        1 attttatttg tgtaaactga ttttaaatcc catttcgaca aacgctttta acattaaaaa
       61 tatatcagcg tcgttcacgt ctgctttttt tcgctcctgg tcgctcaata agtcaatttt
      121 gcgcgtcgtt tcatctataa gctcgactgc gcgcccagcg taaccagcga tcccagcgat
      181 gcgattaaca taatattcat tatggacgtt aacgccagcg ataagaacaa acatgattaa
      241 gctccttaaa aaaaattaaa tagaattttg aagttttccg attaatgcgc ttaaacgtaa
      301 gtattcacgt ttgcgcttgc aatctcgctt gctgttaagc aaatcagaaa acgtaaaatt
      361 taattggtcg cgttgcgcga tcaaatcatc gattaattct agtttataag cgcgatactt
      421 tgcgcgatat tccgcgcgga tcttgtcata gctgaccatc tttaaagctc ctttaaagcg
      481 cccactatgg gcgcttaatt ccattaatta cgctttgaaa gcgtcagcta aataattgta
      541 gaaatcattt ttaatgaagc gatattgctg agtaccagct tttgcgatcc cagctccttt
      601 gactttttca acaagtccaa gacgttcgca aagattgatc aactggttgg cttgagtata
      661 gccagcgtcc aatttaatct cacacgcttt tttagcttca ttcattaaat cgaaaacagc
      721 gccattggtg aacgtgtcga tctcgtcgtt aatcatatcg attaaagcaa atacacgaga
      781 tccagacata tcagcgatgg aataaacgca tttaccagat ttaattgatt taaccagata
      841 aaccagtttt tccagtgaat aactattagt cattgcatca cggaaaaata cttctggcgc
      901 ttgtttgctt gctttaatcg catagtagaa aacagagcat aatttttcgt cttctactgc
      961 gtttacgacg ttgttgatga agtaagcaag tttagtggtc gcagcttgca tatttgcttt
     1021 gtctgcttta gtgtgcgtgc catttttata atgttcgtta tatgtttgag ttgccagatc
     1081 tgctttttcg cgcagctcgt cggatacaat agatgcagct tcaatgatag actttttaga
     1141 aatgataatg ttagccatga ttttattcct taatgtaaat atttaaattt atttgtcgtt
     1201 agcttgtttg ctttcgacga gttcaattat cgatatgcga aaaaagattg caagcgattt
     1261 ttttaaaatt tttgcaggga tgaaagtcct agaaataaaa gcgagatcgt cgaaggtgtt
     1321 ccctaaataa ataattgatt ccggctttcg accttatata tttatacggg gtagaattga
     1381 aaaggtatta aatgaatgac atgtaaaata ataaccggac ttagccggtt attgccctta
     1441 tagaaattag aacggaagga ttcgttcaac ccaatcgaag aaaataagta tttttctacc
     1501 gtcctccaac ttaagtgtga ctagacaggc gtcaacacca tctgacgttc cctcaatttc
     1561 gcgaccgtct gccatgtaga cccttataga tttgccgttt tggtaagcct gacgacatat
     1621 cttaaaaaag tcacgacgtg atggctggtt atctacatag tcaggatgta ttgttagtct
     1681 ccctttgaaa tcgtaagcaa tgccatcaat gacacctgat tcaattgtac taatgcgctc
     1741 aagtgggagt cttatacgat tgtcattatc aagcggtgct ggacataaat ctagcttgtt
     1801 gcgggaagac attgttccct gcacatacat gcaaaacacc tgaccatctt caagtgtgac
     1861 tctgacagga aatttagctt tccgccagaa gagcaaggta cgttcagcat gttcgtagtc
     1921 tcgcggccat acttcagccg gtatgccgta ggtgatgtcc aatgttctca ttctgatact
     1981 cttagaggtg ttttcagcaa cccaaattta aattctttgt taccaacgac aaagaaggga
     2041 acctcaacat agattttttt atcgacggat ttagagttcg ctgttttctg tatgtcacga
     2101 acgatacgtt ttgaggtatt ttcatcgata aacaatacct ttccacttgt attcccttca
     2161 gccttggcgt aagtaacgat agggtcttca ttgccaaccc taatgtttac ttggcaacct
     2221 gttgtagcca tttcatcaca gtaaagtgtg ccgtcttgaa cttctaaaac aattccagat
     2281 ggcttcatgg cttcgtcaga gcttacatta acgttttctg gtacagcggt cccgttaatt
     2341 tgcaaccgta atctttcttt tcctccatta agtattgttt cttctgcgtt gtctgagaat
     2401 ctgaaccaaa ctggctgatt cagacctcgt gatttgtatt ctactacgtc ttcggaggta
     2461 aacatgctgt acgttttttg catgtcagag acgaaatctg ataatgcgtt ggcggaaaac
     2521 gccgggaggt atatgcaagc aaggagtaac gaaagtttca ttttcatatc atgataatcc
     2581 aattatttaa catcacactt tctaagtctt tctatttgtt ctaatttttc atgatcgtca
     2641 tttagatttc cttcgactat ctccgtttca agtttgctga ttagcatgtt aagtgcgttg
     2701 tagttattgc attttacttt gtaggcgtag tatgaccttg tccctggttt ttgtacaccc
     2761 atatcattaa agttagagct taaagtataa ccttcaagtt caaccccttt tattgttgga
     2821 agaattatat ttaatggttc taatttaaat gggtagatat aaatatgtga tatgaagtac
     2881 tcttctgaag agattttctt catgtctttt gtgattgaaa ctgaatattt acggttgcga
     2941 gtgatataaa attttgtaca tttgtacggc tctaagttga atagataata tttgtcttta
     3001 aaaagaacat cttttccatg tgatatttta tgtcgtaaag agttaattat tttgtttata
     3061 gaaatactca tctacagtat ccgaccttag ttatcaattg aaaagcctac aaatatcgtt
     3121 cagccaatca agaaattttt tcagccggag gtgtgtactt caaaaaatgt gatgaatgtc
     3181 acatacaggt gctctcatac acctgcatgt agactattaa ttattcaata atctatattt
     3241 tcttttgatt aaatcttcac cttttttggt gaacacaaaa ccaaattgcg attttttgtt
     3301 tagacgaagg actctgttat ccaacgcttc ggttgatgga atcaatgttt gatcatctat
     3361 ccaattcagt agcgtaagaa agttcagcag cctcaacgtt gttgtgcgaa gctctttcga
     3421 tatattttca atgctggcaa ttcgtctttc ttttttctca ggtggtacag ataattgttc
     3481 ttgaggtttc gtggatagat aatcaatttc gtttgcagca tcgaatgata tgtgtggcgt
     3541 tattttgcta aaaaagcctt tcgctgactc acttgataat aaaccagacc tattcatatc
     3601 gtttgttatt ctgtacagga gtggactgtg tttcatttca ggataacgtt gataaaagtt
     3661 aagcaattca aaagcagcat tagcaccaac aagaaaatca gaaggatcgc tgactttcca
     3721 gaaagaatga tttttcttag cgccagtggt gtcaacaaat gataattctt ttaaccaact
     3781 catgatgtgc ttctttctaa caagtgagca catcatagaa ttagtagata gggggtaaat
     3841 atgaaatgtt acggcaacct gctcagtatt tcctctaaat ctttttttgt catattggag
     3901 ttttcataga tgcgcatgat tttctcacgc gtcttagcgg aaaccccaac ggcagatgtg
     3961 actttgtcaa attcagccat agtcatcgtc tccagaatgg cattgataac ttcagcctta
     4021 gacatcttga tttttttctc tttgagtttt atttgaaatt ttccaagttt ttcattggcc
     4081 ttatcagaca atgccacttg gcaataagtt gttttctttt cactcataac taatctcgtt
     4141 tcagaactcc aaaatcgaat gcgccatcaa caggcaatac accttctgca aagccaggtg
     4201 tggtgtcgat gatgtgtttt cgctcataag agtgagacaa caggtatttg ttgctaatgt
     4261 caatgaaatc agtgataaaa cacacgtttg cctgattctt cttggctcgt aagccacgac
     4321 cgacacgctg tctcatttca acttctgctt tcccaccacc agccagaatg accgcaccaa
     4381 cgcttggcac atcaacaccg acatccagaa tagtcgagcc tattaaaaca tctatttcgc
     4441 cagacgctaa actgttcagc tttgcttgcc ttgtcgcctg gttcgattcc ccatagatga
     4501 agtcaactat aaggccggac tccttcatca tctccatcag gatttgcccg tggcgtttaa
     4561 gacgaaccag agtcatgcaa ttgagagaat gttgcttata gagcaatgct tcgcgcacaa
     4621 tggcctcgtt acgtcccaaa ttatacacga tccccaactg ataagccttt tggtaggcgg
     4681 tgctcatacc aactctaaaa ttgaggtgtt tgttggcaag ttcggccttg attctggcct
     4741 cgtctggctt gtaggcaact ttatgataaa ggaagtacgg ctttgccaga atgcctcgat
     4801 caatcagata cttttctgtg actttaattt caattcgccc ggccatcgcc atcaggcgca
     4861 tgttggcttc cgtcgaatcc ttcatgaacg gcgtggctgt aagcgccaga cgatagtctg
     4921 cgttcacaca taatctagca atgtcataga aattagagcc tgaagactca tgcgcttctt
     4981 ccagaataag cagagacaca cttgaaagga agcgtttcac caactcccga cgtttgaggt
     5041 ggtagctctt tttatctggt gttgcatcgc gtggtggttc ttcaaggaaa cttgccagag
     5101 tttgaactgt agcgacgttg atatgtcgtg atacctggaa ctcacccgag ccaatgactc
     5161 caaccttttg gccctttaac cacggttcgc cattttcggc gcggtagtcg atggatctct
     5221 ggaagttttc ggccatttga aacatcagaa cagagcgggt tgttaaaaat aatgtcattc
     5281 gaccgatacg tgcagctgct ttgcaggcaa cgttagattt cccgccacca gtagcgatct
     5341 gcgcaatcat cattccctct cgaaccagtg tttccacagt ctgatcctga tacgcataat
     5401 caggattgta tgggaatggg ttaaccgccg gatttggttt accaagcgcc ggggctttgt
     5461 ctttgcggat atgaacacat ttgatgcccg ccttgttcaa attagccgcc acgggcttgg
     5521 caaagccagc agggaacgag tttttgctcc agttgaacat tgtgcttgtg cccttccagt
     5581 cgccagcctc gacttcgtag ctcaacattt gctgcaccag ttgctttacc ttgtcatctg
     5641 cgccagaaat aagcgcattt actgcgttag atacaatccg aacagtcata aacctctttc
     5701 cttagtgcct tttgtatgtt atttggctat tataataagt aagtgattac ttagtggatt
     5761 gtagcaataa aatggacgta aaaatcacga ttttgcaggt tgatgttgcc aaccttcgcc
     5821 cgaatacctg gaacaccaat tcggttggtg cgcagaattt cgaaaaactg aaaggttcta
     5881 tcgaaaaatt gggctttttt aagccaattt tggctcgtga gcttgaagat ggattttttg
     5941 aaatcctcgg cggcgaacat cgctggcgtg ctgctattga gcaaggaatt tcaacggttc
     6001 ctgtgctttc tgtgggcaaa attagcgatg tcgtggcaaa acagatgtca ctggtggaca
     6061 acgagcgata cggtgaagac gaccaaatcg cattgcaacg cttcattgaa gaaattcagt
     6121 cagaacttga ctatcaactg tctgaaatcg ccccgtatga cgacgaaatc tcgatggttt
     6181 tagcaaaaga ggcggcaatc gaccttgaag cactggaggc gttgtctcgt ggtagtgatg
     6241 agcctgtcga taccgacaaa cgagagaaaa ccgaacgtgt cggtgcggaa catcagacca
     6301 tgcgcttcaa agtaaccttc gatgcgtcag atcgcgttgc agaaaccata aaaaacatca
     6361 tcaaagacca ggcaattaat actggcaatg aaatggaaaa cgctggtgag gctctagtgt
     6421 ggctggtcga ctactacaag gagcgtatgt aatgaccaaa aagtttgaaa tcgtatatcg
     6481 cgacccggca gatcttattc cctatgagat gaatgccaaa aagcatgatg aacagcagat
     6541 cagagatctg gccgcagcca ttaaaaagcg cggatttgac cagccaatta cggtcgataa
     6601 gaacgacgta attattactg gccacggccg ccgtgaggct gcaatttttg ctggacttga
     6661 gcgtgtaccg gttattgttc gcgatgatct cagtgatgac gaggttcgtg cgaagcgcct
     6721 tgaagataac cgacttgcca gcattgatta cgatgcaatt aagctacaga aagagcttga
     6781 gtcgcttgtt ctggacgata tcgaggtttt cggctttgaa gagcgtgagt tgaatgttct
     6841 cgttggcagt atgacagaag aaatggacac cgactcgcta gttatcgatc ttagcgaaga
     6901 aactaaacga cagaaggatg aacacaccga gatcagtcgt gaagttgcag cggaagaagt
     6961 acgtgttgtc gacgtattgg gctttaaaac tctccctgct ggctctgcca ttgttgttgg
     7021 tgatttgctt gcccatatgg aagaaatgac gggagaaagc ggggtagacg catttgtggc
     7081 atatgcggag aagatctctt ccggggagat ggctgcatga gcaaatacaa catcaacgta
     7141 tcgtttcaga cacgcgtaaa taaaaccacg cgcacgttgg aaatcgctga gtcgttcggg
     7201 cttggcctgg acgaaaaaga gtggacgctt tacgacaatc tggagctgga agtgaagcag
     7261 ggcgatgtgg tgtacatcac cggccaatcc ggttccggca aatccgttgt gctgcgcgag
     7321 ttgcaacgac agatgaagga tgaagggctg tctgtagcct ccatcgatga ttttaccttc
     7381 gataatgagg ttaacgtcat cgatcagttg ggcaaaacga ccagcgatgc gcttgggttg
     7441 ttatctatgg ctggtctgaa cgatgcatat ctgtttgttc gcaagccttc tgaaatgtca
     7501 gacggccaga aatatcgtct caagattgcc aaactgattg agtcaggcgc taaagtgtgg
     7561 gctgctgacg agttcggtgc tgttctagac cgtgtaaccg ctcaggttgt ggcatctaac
     7621 ctccagcgtg ccgctcgaaa ggttggtgcg acggtaatgg tggcgacgac tcacgaagac
     7681 ctgaagaacg cgctgcgccc ggatatgcag atcaccaagc actacaaaga acgcgtgaag
     7741 gtggaatatc acaatggtag tcatgatgag gtccatttat gacggacatc atcattaaac
     7801 gctaccgccc tgaagagttt ccgcgtcatc tggactttct ggagcgaatg actgttacaa
     7861 agggaactgt agaggactgg cacgctctta agtcgcttca ctacaaaaca gacggcaaac
     7921 ctttcgcgcc aacttactat cgctgcgaac ttgatgatcg tctggtgggc gtcgtggtta
     7981 tggcttaccc gaaactactg ttggcacctc gccaccgcat gtttcctaag ttgaaaccaa
     8041 ccactaatac caccgtggct aaccagtact ggggtcggta cgtgaataac aactttgcgg
     8101 tgatcagtcg ctcagttgtg gatactcagt atcgtggcgt aggcgtctct tatcgaatga
     8161 ttaacctggt tagcaggatg catgaccggc caatcattga gatccagtcc tcgatgagca
     8221 aatacaatcc cttcgccatg aaagcagggt ttaagttcat ccgccctgag cgaccgaaga
     8281 gctatgaaag tgcactgcgt gtattccagc gccatttccg ttccgacacg ggtgataacg
     8341 aggcgatcgt caaagagttg ttcgcaatga gcgagtctcg tcgtcgccgt gcactgcgtg
     8401 atctggtggc ggactaccac aagaacagtt ccctggcaaa agctgggcgg aatcgtggca
     8461 cgacgattca ggacattgcc gacagtctgg tggacgaggc cagcattgtg aagctgctca
     8521 aggacattca caacctgagc tttacgtctc cgttgtatgg tgtgtatcga aaccctgact
     8581 ttggtcgtcg actgcctgac acgctgccat tgctggcatt cgacaaacaa cctttggata
     8641 aaccgttaga aattgcttta ccggcataag gatttgccat gacgttaacc gataaacaaa
     8701 aggacatcat caaaacgctc aatctcggtt atgagcgagg tcatctactt gacctggacg
     8761 aattgcttga agttttgccg tacaagacaa ccaagcaaag tatccagttc tcaattcgcg
     8821 ctctgataaa aaaggggctg gtggagaaag ggcatacgcg ccaacgcagt gacaatcgct
     8881 atcaccgccg aactcttggg ttaaccactt taggtcgagc caaagcgaag ttactggtga
     8941 tgtaatcggt ctgggagctt atttaaagac ctgcatctgt atatataaat aataagtaac
     9001 ttattaaata tatacggaag caggcttcgt aagacatgcc agacctaatt aaacacccca
     9061 gaaaacaagt tggttagcag atgcagtaaa caagttgttt tagagcgcat ggacgcgctc
     9121 tgtgtgtttt agagggatct atgacggtcg aaaaagacga ggtaaaaact cgcctgacac
     9181 cagcggagtg ggccgaagct gaagccaaat ggacgtcagg cgaatataca ctctcaaagc
     9241 tggaggaaga gtacggcatt cgtcgtgaaa cactctccag acatttcaaa aagcgaggat
     9301 tagagaaagg cgcggactct gttgggaaga tggttcgtga gtctcttaaa tctgacgcag
     9361 agcttcgcgc taaagcccgt gcggaaaaga tagaagaacg tcgtacacgt tatgacggct
     9421 gggcgtatgc gttggggcag atggtgatgg tcgaagtcac tacggccaaa cgtgagggta
     9481 agcctttagg ggcgattgag gattctctca agagcttaca gagagccagt aatacccttg
     9541 caaaatgctt tgaagtttcg tccaaagcat tgggcatgga tcatgcggaa aatgacgagg
     9601 aagaaattcc gaacctggta tttggtgagc ttacgccttc ccaggtggcg aaattacgtc
     9661 aggaagacga cgagcctgaa atcatcgatg acgaattgct tgagacgttg gaagaagaag
     9721 ctctaagcga atttgatgcg acagatgatg gaagtgaagg ggaggacaaa taatggcaat
     9781 cccgtcctcg ctcagtcttg tgcaactgca ttctggacag atgaaagtct tccagtctcc
     9841 gcatcgattt aaagttgttt gtgctggtcg acgctgggga aaatcccggt tgtcgatctc
     9901 cactattatt cgtgcggcgg caaaggaaaa aaagcaaagg gtctggtatg tcgctcctac
     9961 ttaccagatg gctcgccaga ttttgtggga cgatctacag gaagttctgc ctcgtaagtg
    10021 ggttaggaaa aagaacgaca ccacgatgac aatcgtgttg aagaacggtt cggagatcgc
    10081 cctcaaaggt gctgataagc ctgacactct gcgcggcgta gcgttgcatt ttgtagtgct
    10141 tgatgaattt caggatatga aggctgacac ctggtacaag gtgttacgac ctactctttc
    10201 atcgacacgc ggcggtgcac tgatcattgg tacgccaaaa ggcttctcgg aatttcacaa
    10261 actgtggact ataggccaga acgtagagct gcaaagaaag ggacagtgga agagctggca
    10321 gtttgtaact gccgattctc cgtttgtacc tacggcggaa attgaagctg ctaagaacga
    10381 tatggacccc aaatcgttcg ctcaggagta cctggccagc tttgagaaca tgtccgggcg
    10441 cgtttactac ccgttcgatc gtaacgtgca tgtaaaaccg cttcagttca accctcggtt
    10501 gcctatatgg gtagggcagg acttcaacat tgacccgatg tcttcagtaa ttttgcaacc
    10561 tcagccaaat ggtgagctat gggcaattga tgaattggtg ctcttttcct ctaacacggc
    10621 agaagtttgt gatgagcttg agagacgctt ctggcgctgg aaatcacagg taacggtatt
    10681 tccagatccg gcaggtgctt atcgccaaca tgctcgtggg gagtctgacg tagacatatt
    10741 caaagagaag ggattcttac gtgtcgatta ttcgaaaaag cacccgccaa ttgcggatcg
    10801 tgttaatgct gttaaccgaa tgctgatgtc cgcatctgga gatatccggc tgtatatcga
    10861 tccgaagtgc aagcatttga ttgattcact ggaaaaagtc atctacaagc ctggaacacg
    10921 agatatggat aagacaggtg gcattgagca tagtgcagac gcattgggct atccagtaca
    10981 tcgtaggtat ccagtcaaaa accgtgttat tcttggtggt tctcgatagg taagtaatta
    11041 tctaaggtta ttcaaatgga tttgaacgac aaacaaatta aggatctggt ggcgcgacgc
    11101 cacccggaat acgaaaagaa aaaagaacat tgggacttcc tcgccagcac ttacgctggc
    11161 gggcgtggtt ggtttacaga caatatcttt cgttacttta aagagggaga tcaggagttt
    11221 aaggagcgtg ttgagcgtgc ctatcgcttc aaccacactc gtgaggtggt aaacctcatc
    11281 aacaaatatc tctttaaaga agacattcat agaaatatcg aagaggcacc agagcagatc
    11341 cgcaatttct ggaaacgtgc gactcgccag aatgcctcta ttgactcatt tatggccgcc
    11401 attgatttgc agtcgtctat ttacggtcgc atatgggttg ttgtcgatag cacgatgagt
    11461 ggtgatgttg agtcagtagc tgacgagaaa aagaaagatg ctcgcgccta cgcctactgg
    11521 atttcacctc agcaaatgct ggatgtggca tgggacgacg acgggaatat gttgtgggcg
    11581 ttaattgtgg aagtcgctcg tgatgacgca gatcctttta cttctacagg tcaggaatac
    11641 caacgttatc gtctgtggac acaaaacgag tggtatctgt tccgtgagga agtgaagaag
    11701 ggcgctggtg gagcaggtcg ccgtcaggca aaagttattt tagaggatag cggtgagcat
    11761 aatctcggcg tagttcctgt gtttcctgtt gattgtattg gagaaagtga atcaccgtat
    11821 ttcagcccat cgttgatcga tgatatcgct tatcttgatc gtgcggttgc aaactatctg
    11881 tcaaaccttg atgccattat tcaggatcag acatttagcc agttggctat accggtacag
    11941 tcgcttttac ctggtgatga aaaccacact aaagtgcttg aaatgggcac aaagcgagtc
    12001 ttcacctacg attctgaagg tggaaaccag ccgttttatc tgtcaccaga cccgaaacaa
    12061 gctcagatga tcatcactac gattaagacg gtgattaacg aaatctacca ttcagttggt
    12121 gtagctggtg agcgaacgaa gcaggacaac gcacagggaa tcgataattc atctggtgcc
    12181 gcaaaaatgt atgacttcca gcgtgtaaat agcttgcttg tcacaaaagc agagcgtctg
    12241 gaaagggctg aacgccaaat catgctactg gttgcgaaat ggatgggggt agatctggac
    12301 gaagaccact ctttaattgc gtatccagaa agtttcgata ttcgtggcct tactgatgaa
    12361 ttttctgttg ccgagaaact gtcattactt caggcaccgg actctgtacg tcgtcaccag
    12421 atggaaatgc ttattgagaa gattttcccg aacattactg aggcgatgaa aaaggaattt
    12481 gataaagatc tcttgaattt tcctccaaaa aatgatctaa atacccttga aaataagtca
    12541 gtacttactt atgatcgtgg tgcagcccaa gaaagcgggc aagatcaacc ccgagggaat
    12601 ggggactcat ctactcaaga gaacgagtga taagtaacaa aaggaatttt tatgaatctg
    12661 tggcaaatgc ttttggcccg tcgtggtctg atggatgtcg ctgaagcgca tgagcgtgga
    12721 ggcgctggcg gtgtagctgc tgataatgag cagagcacac aagatcctga caaacagggt
    12781 gaacaaaaag agcagccgaa gggcgatgac gaatacgctg gcatgactca ggaagagtta
    12841 ctggccgaac ttcgtaaaac caagaaagct ggtgctgaac tgctgaagga gaacatgaag
    12901 cgcaaagaga aagagcgcac attggccgat cagcttgctc agtacggtga tatcgacccg
    12961 gcgcgtgctc gccagctttt agaagctgaa caggccgcag aaaatgcacg tcgtgaggcg
    13021 gagcaagctg aactggagcg tcgtggtgag ttcgatgctg ttaaaaaaca gatgatcgaa
    13081 gcacaccagg cagagctggc acagcgtgac gaacgttata cagcactgga aagtgaaaac
    13141 gcatcactga aatctcaatt agtcgagatg accgtgggcg cttccttcag taactctctc
    13201 ttcctacgtg acaaagttct gatgaccccg gcaaaagccc gcgtgatcta cggttctcat
    13261 tttgaagtgg gtgaagacgg tagcgtagtg ggttatgaca aaccggcagg tcataaagaa
    13321 cgagctgttc tggttgacgg tgaaggtaag ccgttgccgt ttgaatccgc gattgaacgc
    13381 attgtgcggg cagatccgga agctgacgca ttgatgcgta gcgaagccaa gcagggtgta
    13441 ggctcacatt ccaaatcgac ctacacaata tcccaaccga agaacaagtc gactatggat
    13501 aagttgacct ctggtcttgg gaaaattgga cttaagtaac atctaaatca aagggaattg
    13561 atagatgcca ttactgcgtg aagaagctga aaagctatcc aataatgaac ttgaacaggg
    13621 tgtgatcgaa actatcatcg atcgcgatga cctgtttgcg attctgcctt ttatgaagat
    13681 taactcgaag gcatatcttt ataatcgcga agcaacgctt agtgaagcga cctttattga
    13741 tgtaaacgat actattgcgg aaggcgcagc aacattcagc gaacatgttg caaaactgcg
    13801 tattctggct ggtgacgttg acgttgataa attcttggca acaactatgt ccgatactaa
    13861 caatcagttg gctgttcagg ttcgccagaa agtgaaaggt ctggctcgtg cattccgtcg
    13921 aaatctgatc ttgggtaatt ctgaaactaa taccaaagcg tttgatggta ttccgcgtct
    13981 gatgcacaaa gatcagaaga tcgacattgc tggtgcatct atgactttct ctatgttcga
    14041 cgagctggtc gatgcggtga aagatttggg cgctgactgc atcatgatgc gttcagagca
    14101 cctgcgtgct taccgtgcgc tgctgcgtac agtaaacgta ggcccgtctg aaatcatgat
    14161 ggagaacttc ggtcgtccta tgctgtgcca taatggtatt ccatttattg ttaacgactt
    14221 cattcctaaa aatggggtta aagttactga aggttcagag cagaaaacgg aaggccagga
    14281 aggtactgct aacatttatt gtctgcattt gtcagaagaa aatggtctta ccggtctgta
    14341 tggcggtgac aatgctggta tcgttgtgga gaacattggc acggtacaga ataaagatgc
    14401 agtacgtact cgtgtgaaat ggtactgctc tctggcgaat aagcacgata aggctatcgc
    14461 tgcactgaac aatgtaaaaa tttaactaaa acaataggta tgtaattacc tatgcttaag
    14521 ggtgggctat acgcccaccc tttttgtagg ttttaaatat gcctgtatag cgagaaagcg
    14581 taatgaaacc agcaaaaatt cgtttattgg aaccgcaatt ttctgattat tcgggaatgt
    14641 tgtgtggagt taagtttgag aatggggtat ccgtttccga gttacctttt atcgaccagc
    14701 agaggatttg tgcatcaatg cgcgcatcaa ctgttgaggg taaaaacgtt tctccatctg
    14761 ctgcatatgg cgaacgtaat gatctgaacg tagatcagat tgttgagcca tcagcacctg
    14821 acattgttcc aatgaagcgc ggaacggcag atgaaccagc taaaccaatt caaactttca
    14881 cacgggaaga actggagtca attgctgata gtgagggtat tgccggttta cgttcaattg
    14941 ggaataagat tggtgttaag gcgaaaggaa ttgtcgaaat gattgacggc atcctgaaag
    15001 cacaaggcgg tgagtaatgg cgcagatcga ctcgtatcgt agcggtgaag ctgtttctct
    15061 ttcatttgcc ttcaacgtgc tggatatcga atctgccacc tacacagtaa aggacagcac
    15121 cggggctatt ctcgttgatg gcgagccgct agaaattacc agcggtcaaa tgtcgattcc
    15181 ggttgtcgtg tcggctgaat ataaccagct ctctgagaaa gagcgagatc tgcggtacgt
    15241 cattgtgaag gctgttgcat cgggcctgac gcatgaagag cgtcaaatgt atgttctgct
    15301 gaatactttt gaactgtcga taccagaaca gtcgtttgca actgtcgctg atgctcaaat
    15361 gcaggcgatc gatatgctga atggggacac tttgctgtcg gatggtgaag gcttaatgcg
    15421 caagcgtctc attgaggcta caagacgtat taaaacttta ccgttctcaa tccgcaaaat
    15481 tctacgtatc gactttgacc gatacgatcg ccctcaaaat atgctgaatg tgtatgacat
    15541 tccgtgggga gcagatggag cgtaccggca tgatttagtc gattgggaaa agattactca
    15601 ggagaagttt gaagagttcc ctgactactt caaagaagcg ttaatgctag ccgttgttaa
    15661 tgaagcgtgt gagatagcaa atggcaatga tatcgtcgca gcacgagagg atggcatttt
    15721 gtcagaatct atcggtgaaa ctaccaacat gtatcgtacc ggtaaatcag caaacgtgca
    15781 tgttgctcgc agcacctggc gtttactgat ccgttatata gacaatcgta tgattgtccg
    15841 acgttcgtaa tgtctttctc attatttggt tttggaagca atagttttgg ctggcaatga
    15901 gatcgacaac agggagagag cgtgaatatt tcatggcaaa cagaaattgc gatctaccgc
    15961 tttggcacaa agaacgtcta cggtgaagcg caattgcagt tcgtcaggaa gacgaatgta
    16021 ggtgtagtta aattcgaaca gagtaatgag aaatcgtcag ttcgtgctga tagctctggt
    16081 agtcgtggca aggcgagtct ggagctgttt gatgctgtgt tagttgttcc tttggaatcg
    16141 gctgtagaac ttgatgatgt actgatcctt gaaggtcaaa aattaaaggt gtccagtgtt
    16201 catcgccgct gggggctacg aggaagacct gggcatcttg aagtaggggc gaatatatgg
    16261 gtctgaaata cgatgcgcat cagttcaaac gtgcggggaa tagactcaac aacagccaga
    16321 aagcatttaa gcgatatctt attcgggaca tggaaaagct ggcgcgtttg gttgagcgcc
    16381 tcagccgtgc aatggccccg ctggaaaccg gatctctgga gacagccatc tttgctaggg
    16441 tgattaaaga aggttattca gggctgcgca ttgagctttc tgtgtctggg gcaaagccac
    16501 gtcaggggca tcctggcgta gaagtaggtg actatgctaa gtacatggag ctgggcaaat
    16561 accgtctcgg ttatctttct cgaatgaaga acgtgacaaa cccaccaatt gaaggtgtaa
    16621 aacctcgtgt tggtccacac tttctggaaa gagcggtgga aattagcgag aagcagttct
    16681 ccgaagcgat tcttgaggct gccaaaaaag ccgggtttac gagaggttaa tgtgtttatt
    16741 gaagcatttg caaatttgat gcagaaggca aagattggca cggtagggac agacattttc
    16801 tgtcattact tacctgccta tgtcaaatcc ggtgtcctgc ttattaaccc aaataccggt
    16861 atcagtatcg atcatgagtt acagggcttt tatcatgagt cgttcacaat cattgtgcgt
    16921 ggctcgtcaa ttacaacgac tattgagaaa gcgcgtaaga tcattgatat gtttcccgta
    16981 gaggaaacgg aatctggaga ggtctatttt cgacttgtac ggcctatggc tatgcctgtc
    17041 gtttatccga aaaatgatgg atcgttaata gaagccggta ttccagttga atttgctggc
    17101 tatttattga attaaataaa taggtaagta tatacttaaa tatagcgcaa tgaatgcgtg
    17161 aaattaacgg aaaaaggagt ttccatcaat gtctaatacc catgtaaaaa acatcaagct
    17221 tggtgcctgc aaggtatcat ttggtggtgt ggatctgggt tacaccaaag gtggtgttca
    17281 ggttgaagta gccactgaaa cgcttaaagt tacagtcgat caacaaggtc agactgtaat
    17341 ctctgaattg gttcaggggc gtaacattac cattaccgca cctctggccg agtctgtgct
    17401 caaaaacatg gtcgatctta tgcctggttc tactttgagc gaagatgaca actccgtcac
    17461 catcacatct gcacagggcg taaatctgat caacgtggcg aaggaactcg tcctgacacc
    17521 gcaggacacc accgactatg tgttgacgct cccgaaagcg gcaaccgctg gcaacttcac
    17581 tatgtcctac cagtctgatg atgtacgtgt gttctctgtt cagttcaacg cgtaccctga
    17641 tgatgagggg gttcttggca aaatgagtgg cccaaaaccg gtgaaaaacg tgacgattac
    17701 acctgaatct ccgagtgtaa aggtcggggc gacagttcaa ctgactgcta atttcacccc
    17761 agatgatgta gttgataaaa caggcgtatg gtcatctgat aataaaaaag ccactgttga
    17821 tcagactggt cttgtgcgtg gtgtggaaca aggttctgca aacatatcat tcaccagcaa
    17881 tagcggtagc aagaaagtaa ccaaattagt gacggttaat caataatcaa gcaacttatt
    17941 aatctataaa ttgaggctca tgatgggcct ctcttttaaa aggattttaa caaatgacaa
    18001 aattactcga tctcgactcc atcctacctc ctaaaaaaag tgtcaaattt ggcggccagg
    18061 aatatcccat cgttgaaatg acggtaggtc tgttcgtttc cattaagcag atggaaggta
    18121 aagatctgac caatatgtct cctgttgagc aagtgacagc ttatgcagat ctggttcgca
    18181 aggttatccc ttcagtgccc gacgaagttc ttgaaaaact gactgttccg caacttcagc
    18241 agatcttcac cttcgctatg gaagtgattg atgaagaaaa cgaaaaagcg gctggcgaag
    18301 gggcaaagta atttcccgcg atgaatccgg gacaaggacc gtctcaatag atctcggatt
    18361 ctatttcagt cgtgtagttg ctcactacgc cgtgtcgccg ttagaactac tgaacatccc
    18421 tctcacgatg ttctggatgc tcagtcgcaa catcgaccgt ctacgtgcgg aagaggatgt
    18481 ccgcaacctg caagtcgctc tcgctgctca ggcagatggc gagagcgtga aggcgttcat
    18541 ggagggtttg caactcagga ttggaagacc agtcgtaaca gataaagtct acgatccaag
    18601 ccaggatagg gcagaccctg acgccaaaga gcaactgatg caaatatttg gcagaggatg
    18661 acaagggaat gtcacaaaac gtagagttta tcctgtcgct ggaagacaaa cagtttacag
    18721 cgtcaatcga tcgtgcggga aaactgctta ctcgatttgg tgagcaggta acaaagcctg
    18781 ctcaaaaaat ccaaaccttt gaacgctctt tgggttcggt cgcccgtatc attggcgttc
    18841 tggaaagcaa gctcgattct acggcagata aactacagga tgtagctgcc ggttttgagc
    18901 ttgcttcaga tgctacgcgt aaaatgcgtg gcaacatcac cagcctcaat tctggtctta
    18961 aagccctgat tgagcgcgtc gatacgacaa cttcttcagt agataaactc accgcgtcat
    19021 tacgtaaagt gcaatcagag ctaaatgatt tctctgattg ggcgacctat gcaagcaaaa
    19081 gcgcaagccg ttttggtacg gaggtcaaag aagcctctgc gtccgtgagt ggcatgaata
    19141 cgcgccttaa taccacgacg aagcgactca gtaattgggg tgtgacaaca agccaggctg
    19201 ccgagggact aaaaaaagtt cgtgagcaga tggacgaagt tatcggacgt cagcaactga
    19261 ttaacaagcc tgtacgcgtt cgcacatcag gaagcggaaa tggtgggggt agcggtggac
    19321 gtggtggtta ctcttggcat atcggtaaaa ataacgaagg cagtatgttc tctggccttc
    19381 gtggcaacat tttcctgctt ggtgagatcg gagatgctgc aagaaccgtc accagtatca
    19441 tgtttggttg gcagaagcct attattgaag ctgcggccga aatggaacgt atgcgggtga
    19501 tgcttcgagg gttgaataag gagaagtcca acccgggcca ggctgccgct gatgatatga
    19561 agtacatcgt agacatggct caaaatgcgc cgttcgcgat gcaggcgtta acagactcct
    19621 tcgtgaagtt tcgttctgct ggcttagatc caaccgatgg ttctctgaag gcgctggtgg
    19681 actccgttgc tcgttttggt ggtaatagtg agctgttgaa acgtgcggct gtggccgttc
    19741 agcagatgtc cggtaagggc gtcgtgtcaa tggaagagct acgtcagcaa ttaggtgaag
    19801 ccgttcctaa cgcgatgcag gcaatggcgg acgcagcagg catcactatg ggggaactga
    19861 ctaaagccgt tgccagcggt acggttgagg caaaacaggc gttgtctctg atgtttgttg
    19921 gtctgcgtgc ggagaatgag aacgcagcga aagacatgat gcaaacctac acaggtgcgc
    19981 tggcgcaact tcagacgtca ttcacgttat ttgctgatcg ggttggtcag gccggatatc
    20041 tggattctct atcgaagggg atgaaagaac tggcttcaat catgaatagc gccgaaggga
    20101 tttcgtttgc caattcttta ggttcggggt taacaacggc aatcgatggg ttgcgtcagc
    20161 ttgctcaatg gttagcaaag aaccaagagc tggtaattaa tctcggtaag gtcgtggccg
    20221 cgatggttgc gttcaaactg atgcgagcag ggatcatggg ggtagttggc gcaggcagcc
    20281 agatggttag cacctttgcc acgatggcga ccgccataca gactccattt aacctcggcg
    20341 ctacagcagt aactcgattc aatcgtgcgg cacgtatggg gctggctccg atcccctctc
    20401 ttattttcgc catccgtggg gcgattacgg ggcttaaagg cgcttttgct ggattaacgg
    20461 cgttcattgc agcaaatcct ataggggcag catttacagt tgccactgta gctgttgctg
    20521 gcctaatcac gtacatgacc atgctccgca gcgaaacttc aaaggtcgtt gacgagatta
    20581 ggaaaatacc agaggcgatg acggcggcca aacgtgcaca gatggcggag tataaagcgc
    20641 gtcttgagca acaaatcacg caaaaggaac aagagttaaa atctggcgaa aaggtggttt
    20701 atgggccggg tatggccgga actacagtaa agattaatca ggatgaacgt aagcgtatag
    20761 aaaatgagct aaatgacctt cgtaagaaac gcgataaaac tactggggca attgaacgcg
    20821 gagacattgc tgtagccaaa cgtcttgcaa aagatgcggc agaatcacag atagagaaaa
    20881 tacgggaaga gaatagagac tttgcggcaa cattcgtgaa agcccggcag gaggctctgg
    20941 ataagatcca gaaaatcaat gatgacggtt cactttcaga tgacgaaaag aacaagctat
    21001 tggcaccgtt acgtgaaacg gtaaacaaaa gctatctgga gcctgcgcaa aaactggttg
    21061 attcactttc ttctcgtaag aatgctgtcg aggggcaaat agccactctt aatgatcagc
    21121 ttgaaaaagc gaaaaaggaa ggaaacaccg agcagatcaa gaaactgcaa ggaagtattc
    21181 gtggttatca ggagaatttg gaagtcgtcg ctcaggaact gactcaggca gagttcgaga
    21241 gagataacgc tgccaaaact ggtaagggcg taatgtcaaa ccaggggact gttcttgggt
    21301 taggtacaac tgataaagct gcccagaagg cgttggcgca atatatgcga aaccagatgg
    21361 attctgcgac ttatcagcgt actttgcctg acggcactcc gatgatggac ttcgaaggta
    21421 agccgattat tgggcctaaa caactcaaga cgcagcttaa tttgcagaaa gcatccagtg
    21481 ccagctctct ggagaaaatg agcgatgaag agcgtgccgc agccattgct gcactgacta
    21541 aagctcgtga acaggatgca gcagccgccg aaaaagcagc ccaacgatca gctaatgcct
    21601 cgcagcgtgc ggccaagaaa gaacaggcag cgcaacagaa actggcagcc ggataccaga
    21661 aggctctgga caaagccgat cagcttatgg ggcaaatggg tgaaagctct aaggctacgg
    21721 tatcgtttga tcagtctctt cgcgatacaa cgaaatcgct gacagatttg gctaatgcgg
    21781 ttcctaacga gttcatcact caagagatga tcgacaaggc gaaaaaacgt cttgaagacc
    21841 tcaaaaatgc gacacctgaa tatcgcgaga tgtttaatcg ccgcaatgtt gagcagatga
    21901 tctccacctg ggcaccggag tcggattcca ttattagtgc tggctatacg ccgtctcgtg
    21961 aagagaaagt tgctgatttc gaagacacct acaaccgcaa tctcaaagcg ttgatagaac
    22021 ttcgtgataa ggcgtctgat cctaaagttg tggcgcttta tacaaagaaa atcaatcaac
    22081 tgattgctgc tggcaatacc gcgcttatta aagagacggg gactgcgacg cagaagttgg
    22141 cactggaata cgaaaacctg gcagagcaga tcgaaagcac ctggactgat ttgtttagtg
    22201 gcttaactga tgtcctgact gatttcgtta ttaacgggaa gatgagtttc tccagcctat
    22261 ctcagtctat tttgaaagat atcaccaata tggtcgtgaa gtcgcagatc acgctgcctc
    22321 taatgaacat gttggggatg ggaaccaccg cagctggtag ttcacagagt ggtaatttgc
    22381 tgaccggggt tgcttccgcc gttgccaatc aaggggtacg aatgggcaac actgttaacg
    22441 gcgacaagtc tgtaggagaa gccacgaagg agacgtccag ttcggtaact ggattggggc
    22501 aaacaacaca gcagaccacc agcgcaattg gcactgcaac aaatgcgatt ggtagctggg
    22561 tatcagggct atttgatagc actgaagcca aagatgccga gacaaaagcc gtgaaggact
    22621 ccatcttctc gatgcagaac ctcagctctg ttaccggcgc gctgtctgcc gcgtttgcaa
    22681 tgcttggagc taatgcttcc ggctctggta ataagtggtt gagttttgga gcaacggttg
    22741 catctggtct tgtttcagct tgggctggcg gtggtttcga cagcgtgaca tcaagctctg
    22801 ctaaaaccgc aaccagcagt gtggctgacg gaactaaagg cattcctgca atcccgaagt
    22861 ttgcaaatgg aggaatattc ggaaaagacg gcgtgatccc gctccgggca taccaaaaag
    22921 gcggtatcgc caactcacct caattggcgt tgtttgggga aggcgatatg aatgaggcgt
    22981 atgttccatt gcctgatggt cgaacaatcc ctgtaacgct cagtaccgat ggtatgagtg
    23041 gaggaggaaa tgttctttct ccggtatcaa ttgagatcaa cgtccatagt gacggtagca
    23101 caactgaatc cggcgataca gaaagcatat ggaacaatgc cgctcaacgg atgaaagcaa
    23161 tcgcgcttga gactatcgct caagagaaac gccctggcgg atcactcaac ccaaacactc
    23221 aacgtaacta actatcgact gccccgaccg gggtagtctc acaaggatgt gagatggaaa
    23281 gacaaacgtt taattggtat ccagattacg aatctgaaaa aagcgtaaaa ccgaatgtaa
    23341 cggtacttaa ttttggtgat gactacgagc agcgacaggc tcaaggtctt aatcgtatta
    23401 aagaagaatg gtcgttaacc tttaccagat catacaacga aattaatgca atcgatgact
    23461 tcctgactga gcgatcaggc gttgaatcgt tctattgggt taatccaaga ggcaagcaga
    23521 ttgtagttgt atgtgacagt catacggtca agcgatatca ggggtactgt gtcttaactg
    23581 ctacattcag acaagtattt gaggtttaag catctggata agtaagtgct aatttactat
    23641 tattgtggcg ctgacaggat gtcagcgcct acttatttca aggatgaaac aatgggaatt
    23701 aaagctgata ttcagagctt atctccctct gcactcattg agttgttcgt actggatatg
    23761 tcgaatacaa cttcaggggg gaagctattc ttccacgccg gaacaaacga actgatgcaa
    23821 ccggtcgtct ggcaaggagt gacatacgag ccgtggccaa tcaaagcatc aggttttgac
    23881 aaaacgggcc agggaacgtt gccacgtcca aaaattcagg tatcgaactt tgccggaacc
    23941 gtctctgcgg aagttcaggc aaacgacgat cttgttggct gccgcattat tcgcaagatg
    24001 acgctggctc gcttcctcga tgccgttaat tttaaagacg gcaacccaac agcagatcca
    24061 aaccaacatt tccccgatga aatgtggttt atcgaacaga aaactctcga aactcatcag
    24121 gttgtcgagt ttgaattgtc cagtgtgttc gatttgatgg gggtgcaact gccgtatcgt
    24181 cagatcatta aaaacacctg cccgtggaaa tatcgagggc cagaatgcgg ctataccggt
    24241 ccatatttcg acaaaaataa ccagcagacg tctatgtctg gtgcggacta ctgcacaaaa
    24301 cgttatgacg cctgtaatgc gcgtcggaat tattttgccg acggtgtgat ccattttggc
    24361 ggatttattg gagctacgcg gtatgggtaa taaagcaatc cctgagcttg gctctgacgt
    24421 tatgcagcaa atctatctct gcgccataaa tcgctaccct aatgaagcgt gtggctttct
    24481 ggttagaact aatggcgaca aatatcgctt tatggaagcg cggaatgttt cggagaaccc
    24541 gcagaacact tttgtaatgc acgttgacga cattatggcg gctgaggatg cgggtgatgt
    24601 tatcgcaatc tggcattcac atactgatga atcagcagaa gcatctgatg ccgatcgtgc
    24661 aggctgcgaa gcgacggaag ttccgtggat gattctggct attcgcaaga atgttgaggg
    24721 agatgcccct ttccatttta gcgagatgaa tgtgatcaca cctgatggtt tcgaaatgcc
    24781 atacctgggt agaccgtatg tatttggcgt attcgattgc tggatgttgt gtcgggacta
    24841 tttgaagcgt gagtttaacg tcgaactaaa cccgaacgca cacctgcata ttccatcgtg
    24901 gtacaccggc gataacgaca ttctcgacca gaactaccga aacgaaggat tggtacggct
    24961 tgcgcccgga acagaacctc aacgtgggga cgtcttcttt atccaatacg gaaaaatgcc
    25021 tgaccactgc gcggtttata tcggcgatgg aatgattctt catcaccaga tcgaccgcct
    25081 tagttgtcgt gcttattacg gcgggatgta tcagaaacat acgacgcatc acttgcgtca
    25141 cagagacttg ctcaagggag atgaggcgtg tctgagttag ttcatgtgca gcttggcggc
    25201 cctatggcca aacattttgg ccgccactgg catctaaagg tgcgcaatac aaaacaggct
    25261 ctggatttaa ttgaggccaa taagcctggt tttaaagcat ggatgaagcg caatatcaaa
    25321 acctatgaca gataccacat ccagatcacc aataaacagg gccacaagtg gtctgtggac
    25381 gagagtgaat atcagatgat ggggcagtct gacaacattg ccaaaatccg cattacccct
    25441 gttccgcgag gaagcggtgg atctgctttt gggtggtttc agacggttgt tggagcagtg
    25501 ttaatggttg cctcgatatg ggtgcccgct cttgcgcctc tcggtttgtc actgatgatg
    25561 ggcggtatag cgcaaatcat atctccacaa gccactaacg aaagtgtgag acaggctgat
    25621 aattcgaact ctttttactt tgatgggcct caaaatacaa caaaccaggg caaccctgta
    25681 cagcttatct atggtgagga aattttggtt ggctcacagg tcgtgagttc ttctatcacc
    25741 atcgaccagc ttatgtaatc aaggattttt gggcatggaa cagttcaaga agaaaaagtt
    25801 acctctatta attgcaggtt ctggtggcaa aaaaagtagc aaaagctcta gtcgcacacc
    25861 agttgaagca gacgataccg taaattctcg tgctatggcc tctattctcg atcttcttgg
    25921 agaaggcgta attggcggct tggtaaatgg cgcaagatct atttttgtcg acgatttgcc
    25981 catagtaaat gaagacggct cttcaaactt tagcggaatt tcatgggatt ttcgagacgg
    26041 ctcgcaagac cagacaccaa tgtctggctt tgatttcgtc gaaacaccta aatcgatcaa
    26101 tatccaacta aagaaatcgc attatgtgac ggtttcaata gataacgatg aggccgatcg
    26161 tgttcgagtc atcatgaagt tcccttctct gcgaagaata gaccaaaaaa cgggtgatac
    26221 aaacggcact accgttgagt ataagttcca gatctcaaat ggagattcaa catttgtaga
    26281 tgtggtcgca gaaggtgaaa aaaacgttgg tattaagtta acagcgaaga aaaccggtgc
    26341 ttattatcgt agttatgagc tgaagttacc caagccaggt cgggcataca gtgttcgagt
    26401 agttcgtatt actgatgata acagcggtca gtatctttat aacgatacat gggtggactc
    26461 aatcggtgag atcgttgata caccgatgaa ctacccaaac tctgtgttgg tgggactgaa
    26521 ggttaattct gagcagtttg gcggctctat gccttctcgt tcctatctgg tgcggggatt
    26581 aaaaattcgc gtaccatcaa actacaacga ggctagtaac acttatgatg gcgtatggga
    26641 tggctctttt aagctgttgt catcttcaaa ccctgcgtgg attctcttcg acttgcttac
    26701 caattctcga tatgggcttg gtcagtatgt gtcggaatct atgattgatc tcggccaact
    26761 gtaccagatc ggtcgatatt gtgacgaaga ggttgatgat ggctttggtg gtaaagagaa
    26821 acgctttgca atcaatacgc agatcaccag tagacaggat gcataccgac taattcagga
    26881 tattgccggt gcattccgcg gcatggtgtt ttgggctggt ggcatggtta acatcatgca
    26941 ggatagccca tcagatccag taatgatgtt taccaactcg aacgtcaaag atggattgtt
    27001 tacctataaa ggttctgcgc gtaaagatcg cccatccgtt gcgctcgtga cctacaacaa
    27061 caaggaggac ggttataagc aaaacattga gtacgttgaa gatcaggacg caatgcgccg
    27121 ttatggtgag cgtaaaacag aagtcgtagc atttggatgt acaagtcgag gccaagctca
    27181 ccgagttggt ttgtggcttt tgtataccgc cagaatggag tcggatgtaa ttacatttac
    27241 tgccggttta gacgcctcat ttctgatgcc cggtgaaacc gttctgattc agaacaaata
    27301 tcgtgcaggt aaacgtaact ccggtcgaat tgtggagttt accaaaaaca gcatcactct
    27361 cgatgcgcct gtgtcgttag ctaaaagcgg cagctttatt cggatattga atcaggaagg
    27421 caaaatcgtt gaacgcgatg ttcttgaaac tggcgaaaac ataacaaagg ttacgttttc
    27481 aaaagctctg tcgtcagcgg aaacgcctgt tttgaacggt gtatggacaa tcacagaacc
    27541 agatctcgaa cctatgcgcg ttcgcatcgt taacatcgct cagggggaaa cgtcgggtag
    27601 ctttgacatc accgctgttg agaacaatcc gtctaaatat gaggcaatcg acaatggtgc
    27661 aacgcttatc ccgcagaata cgacggtatt ggacccgact tactccaagc catctaattt
    27721 gcaaatcact gaagggactt atctctcaag ccctggcaac ctgtcagtaa aactgactgc
    27781 aacatgggaa gggaaatctc cagagtattg gatcagttgg cgacgttctg atgaaaacaa
    27841 tgtatcgaac tggcaatcgg cacgtgtaac cgaagagcaa tacgaaatcg ttaatgtcgc
    27901 ggagaatgga cgctacgact tccaactgta tgcggtttca ttcaacggta aaaaaacaga
    27961 gattatcagt accgtttatc aggtgctggg cacaatgaca ccgccggatg caccaacgtc
    28021 attaacggca gttggggact atcgtaatgt gatactgaat tgggtcaatc cagattcggt
    28081 agaccttgat cacattaatg tttacgcatc ccagaccaac aatctggata cggcgaaact
    28141 gatcgcagag tctgcaagca ccacctttac acatgctggt cttggggata gtgaaacttg
    28201 gtattactgg gttagatcag caaataagcg tggcatgttg agtcgcccaa actcaaatct
    28261 tggtacagag gccacaacgc gagatgttct ttcattttta gccaataaaa ttacctcgtc
    28321 agaacttggc caggctttaa atgaggaaat tgacagtaag gcttctcaac aagctgttga
    28381 tgaaatcaat aatcatatcg atcagagtgt cgaatctctg gaaggtgaaa tttccgcatt
    28441 aaataaggag ctaacgagta gtatcgatga gttcaaaaca tcgtttactg aacgcagcga
    28501 gacactggag aatgcgcaaa gcgagcttag aacggaagtt tctagcactc gggaaaaagt
    28561 agatgatgca ttacgacaag ttgaaaattc aaatgcagca cttattgaac tacgtaatac
    28621 agtatcaaaa caaggtgatt ctgttgctgg tgtcgtagaa gcagcaaatg cggcacttga
    28681 aaatgcatca tctttaattg ccgaagagcg taaagttcgt gcacaagcag atctagcgaa
    28741 tgctaaaaag atagaaacga tgagatcatc ggttgatgaa agtgttgcgg ctgtagaaga
    28801 aatgcggaga acggttgctg aggtaggaag agtaagttca gaaatcacaa ctaatcttga
    28861 agccatttct aagacaaata tcgatcttgc gttgcggcag gacgaagatc agcacaggca
    28921 gctggtaaat aacgcgaaaa ttgccacgac tcaaaagatt cttgtcgatg atgtgtcagc
    28981 aatggcgtct aaggttgaag agatacgagc cgagattggt gacaacattc gagcatctat
    29041 tctcgaagaa gcgacagcca gaactgatgg tgataatgcg ttagccaatc gtgtgacaca
    29101 attacagagt aaatttgaag gtgatatcag tgccgcaata aggaccgaac aatctgcaag
    29161 agcatcggcc aatgagactc tttcaaagca gatcacacaa ctggagtcta aagtaaatac
    29221 tgatatttca accgctatta gagaagagca acaaacaaga gcaacgcaag atgaagcatt
    29281 atcaaggcaa ataactcaac ttcaaacaaa agttaataac gatatatctg ctgctattaa
    29341 aagtgagcaa caagcgcgag ttagcggcga ttcagcgtta tccacacaaa ttaatcaatt
    29401 gcaggcaaaa gtagatgatg acatatcagc tgctattaat gaggaaagag aagccagagc
    29461 atcaggcgat agtgcactat caagagaagt tagtagttta aaaacaaaaa caggtaaaga
    29521 cattgcagcg gcggtagcag ttgaaaccaa agcaagaact gacgctaacg atgcattatc
    29581 cagacaaatt tcttctctta ctacgagggc taataatctt cagtcctcga tagagcgaga
    29641 atcgacatct cgtacaagtc aggattctgc cattgcaaaa gacttggcaa gttttaaaac
    29701 aaaaactgct aaggatataa gtgcagctgt tgcggttgaa actcaagcaa gagctgccgc
    29761 tgataccgca ttgacgtcgc agattaactc actaaagaca caaacaggta aagatataaa
    29821 ggctgctgta gcagaagaga cgaaagccag aactgatgct gatgcagcgc tttcgacaag
    29881 ggtaactaat ctccagtcgc aaacaacaaa ttatatcaat gcagcgatta cgtcagagtc
    29941 aaaggccaga acaagtgcag acaacgctct aggaaaacga attgatacac tgaaagcgtc
    30001 tgtagatgga aatgttgccg ttattcagca gcaggcttct gctatagctg acgctaatag
    30061 aaaagtatca acctcgtaca cattgaagat ggaaacttca acgagtggtg ggcagaagta
    30121 tgtagcgggc attgcgctgg gtattgatac aacggggctt tctcagtttt tggtgcgggc
    30181 agatcgattt ggtttggtca acaatgttaa tgggaagatc acaacgccat ttgttatcga
    30241 aaacagcgtt gcgtatatga acggtgctta tatcaaagat ggcactatca caaacgcaaa
    30301 ggtaggtgac ttacaatcaa caaacttcgt aagaggacga gccggatgga gatttggcaa
    30361 aaacggaacg cttgagatta acggtaacag tggaggaaat gggagattag ttatcaatgg
    30421 gcagagaatc gacgtttacg acgacaataa tgtactcagg gtaagaattg gtcgactttg
    30481 attatggaaa aaaatatttt tatcgataat aataggtagt tacatactta atggtgggca
    30541 aggatagccc acctgattaa aggagcagat tcaacatgtg gtacagggaa ggtactatca
    30601 catttacaca aggcagcaat actctggttg gtgctgggac agcatggaac gttacggcca
    30661 atggtgtgtt gcctggcatg attgtcgtcg cacctgataa taaattgtat gaaattaaaa
    30721 gcgtaacaaa tgacacaacc ttaacgttgg ttgaaaacta tactggtgaa acgcaaagtg
    30781 atataccatg taggatcatc actacctacg aaggagattt gacacagttt agtgcgagat
    30841 ttacggctct tttatcacga atgtctggtg atgcaaaaac agttcgaaat tggttaactg
    30901 ccgtagatga agttactctt gagcgagaag atggcactaa tgttaatgta aaacctcttt
    30961 ctaagattat cgcggagcat aataagcacg tagaatggta tgaaaataat actaacgcaa
    31021 ttaattccgc aggagataaa gcaaaacagg ctgcggccag tgcgtcggct gcggcgaaga
    31081 gtgcgaatct agcaagcagt aaggctactc aggcatctaa tagtgcgttg gatgctacag
    31141 cgtctaaaaa tgcagcaaat aaaagtgcat caagtgctaa agcatcagaa acaagtgctt
    31201 tagaggcgaa gaacgcagct gcaatttctg catcaacagc ttccactaag gcaacagaag
    31261 ccacaagttc ggctaatgct gcgttggtct ctaaagaggc tgcaaaatta tctgaaatga
    31321 atgctgcttc tggtgctggc agtgcatcaa cttctgcaaa tcaggctaga gagtcggcaa
    31381 ggtctgcgaa gacatccgct aatcaggctt caacttatgc gtctgctgct agagcttcga
    31441 aggatgaagc tagccgaaag tctgctgaag cctcaactag tgctagtaac gcatcatcga
    31501 aatcagctga atctgcggct aaagcagaag aagcaaagca aagccaaaca tcagcacttg
    31561 catcggcaga gcgtgcagag atagctgcta ctagagcaga aagagctttg tcagcgatat
    31621 cattacatga tgccagtttg actatgaagg gtattgtcaa attaagcagt gagacgagca
    31681 gttcgtcaga agaatttgca gcaacaccaa aagcagtaaa ggctgcgttt gatcttgcta
    31741 aatcaaaata tactgcaaat gacgcgacaa cttcacaaaa agggctggtt caattaagta
    31801 gcgatacaaa taacgcctct gaatcattgg ccgcaacaac aaaagcggtt aaaagtgtaa
    31861 aggatcttgc tgatagaaaa gcagcaatca acagcccagc atttactggc acaccaacgg
    31921 caccaacggc gactcaaggc actaataata cgcaaattgc cacaactgct tatgtaagag
    31981 aagcgatctc aggtcttgta ggatcatcac ctgaagcgct tgatactctt aatgagttgg
    32041 caagggcttt gggtgatgat cccgattttg ccacaacaat gacgaacaag ttggccacaa
    32101 aacaacctct tgatgctacg ctgactgcac tggctggact aacaacagcc gctaataaac
    32161 ttccttattt tacagggaat gatacagctg gattagcaac aataacttct gttggtcgag
    32221 atattctttc taaagcaagt gtacaggcga ttattcagta tcttggattg aaggaaaatg
    32281 gaacaagtgg cgataaaata cctttattga gtacagccaa tacatggagt gcaaagcaaa
    32341 catttagtaa tggtcttgca ggctcactta acggaaattc cgatactgca acaaaattaa
    32401 aagcagcacg aaaaattggc ggagtaccgt ttgatggttc atcagatata aatctaccag
    32461 gtgtaaactc aacaggaact cagagtacta gtggaaacgc tgcctctgcc actaagttag
    32521 caacacctcg ttcaattggt ggaatgttat ttgacggcac tgcaaatatt gacttaccag
    32581 gcgttaacaa ggttggaaat cagagtacaa caggtaatgc agggagcgca acgagactgc
    32641 aaacggcaag aacaataggt ggggtatcgt ttaatggcac tgcaaatatt gacttacctg
    32701 gtgtcaataa agctggaaat cagaacacgt caggtaatgc tgcaacggcg acaaaacttg
    32761 caacggcacg aaaaataaat ggggtcagtt ttgatggctc aaagaatata agcatcacgc
    32821 caaaacaact aggtgcagca aaatatgttg ctaatcttgc tacggggaat actcaagagt
    32881 ggacaactgc acaatttgtc gcatggttaa attcacaagg ggcatttgat gcaactcagt
    32941 ggagcacacg ttgtagctgg tcttatggta gtaactgtta tataggaaat gcatcaacgg
    33001 gatgtggagt tatacctctt gctggttctg ttattgaggt gttttcaacc ggtacaagta
    33061 attacactat acgcattaca acaccttcaa catctggagg tgcatctggt gctgttctta
    33121 atagtgagtt tatttataca tacaacggcg ataactatag cccaacatgg cgtcgtaatt
    33181 acagttctca aaataaacca acggcttcag agataggcgc ccttgcctca aatggaaatg
    33241 cagtttcggc tacgaaactg aaaacagcga gaaatatagg gggggtatcg tttgatggta
    33301 cagcgaatat agatttacca ggcgttaaca aagctggaaa tcagaacaca tcaggtaatg
    33361 ctgcaacagc tactaaatta aaaacatcaa gaacaatagg tggagtatca tttgatggta
    33421 gcgcaaacat taatcttcca ggtgtgaata cgtcaggaaa tcagaataca tctggtaacg
    33481 cagcatcagc aactaagcta caaacagcac gtactataaa tggagtaagt ttcgacggaa
    33541 caaaaaatat tagtataagt gccgctcaaa ttggtgcttt gcctatcggt gggggtacga
    33601 taacaggtaa tttgactgta aatggaaatg gctcatttaa tgatgttcag atccgctcag
    33661 ataagcgtaa caagcgtaat ctggtaaagc tagataatgc gttagatcgt ttggaggcac
    33721 ttactggtta tctttatgaa atccaactct ctgatgatag ttggaaaacg tcagttggtt
    33781 taattgctca ggatgcacaa aaagcattgt ctgaactggt aactgaagac acagacgtta
    33841 tatctggtga aaaacgtctg tgtcttaact acaacggcat aattgcattg ttagtcgagg
    33901 gctttaaaac acttcgtcat gagattaaag aacttcggga gaagtaaacg acagctgttg
    33961 gagtttctgg tttctactga atttaaattg tgggggtgat actcacccca cgcattcaga
    34021 aggggagatg atatgggggt aacatcggga tgggtcggtt cttcggccaa gaacgaaacg
    34081 ggcgaacaat ggatgagcgc cgcaggtagt aaattaggac tgggtaaacc ttttatgatg
    34141 agtcaaatgg tgggaagatc tatgggctgt aaaattgcaa ccgcttacta taaagggaat
    34201 cctcctgata aagtcgaaaa ctggggggct gtaggttcag attggccatt ggctgaaaag
    34261 aaccaaggta caattgctgg tgcttcgagc tgtggtttag gtcgtttagt ggggatcgtg
    34321 cttacgcaag ctcactacgt tcctggttta ccgcccactg ggggtttata catcgcaggg
    34381 ggaagggcaa gcaatattac agtaacagtt ggtggagtct cacaagtttt tacttacaat
    34441 agtgttatta acggttttca ctattactgg caaggaacac ctagttcggc ctttataacg
    34501 gcaa
//
LOCUS       DAZPAC010000019        33296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZPAC010000019 DAZPAC010000000
VERSION     DAZPAC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.019"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(134..274)
                     /gene="yjjY"
                     /locus_tag="ACHUPM_000769"
     CDS             complement(134..274)
                     /gene="yjjY"
                     /locus_tag="ACHUPM_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR0868637.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            370..1086
                     /gene="arcA"
                     /locus_tag="ACHUPM_000770"
     CDS             370..1086
                     /gene="arcA"
                     /locus_tag="ACHUPM_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR0868638.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1146..2498)
                     /gene="creD"
                     /locus_tag="ACHUPM_000771"
     CDS             complement(1146..2498)
                     /gene="creD"
                     /locus_tag="ACHUPM_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR0868639.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2556..3980)
                     /gene="creC"
                     /locus_tag="ACHUPM_000772"
     CDS             complement(2556..3980)
                     /gene="creC"
                     /locus_tag="ACHUPM_000772"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR0868640.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3980..4669)
                     /gene="creB"
                     /locus_tag="ACHUPM_000773"
     CDS             complement(3980..4669)
                     /gene="creB"
                     /locus_tag="ACHUPM_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR0868641.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4682..5155)
                     /gene="creA"
                     /locus_tag="ACHUPM_000774"
     CDS             complement(4682..5155)
                     /gene="creA"
                     /locus_tag="ACHUPM_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR0868642.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5366..6235
                     /gene="robA"
                     /locus_tag="ACHUPM_000775"
     CDS             5366..6235
                     /gene="robA"
                     /locus_tag="ACHUPM_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR0868643.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6232..6879)
                     /gene="gpmB"
                     /locus_tag="ACHUPM_000776"
     CDS             complement(6232..6879)
                     /gene="gpmB"
                     /locus_tag="ACHUPM_000776"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR0868644.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6931..7452
                     /gene="yjjX"
                     /locus_tag="ACHUPM_000777"
     CDS             6931..7452
                     /gene="yjjX"
                     /locus_tag="ACHUPM_000777"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFR0868645.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(7537..7863)
                     /gene="trpR"
                     /locus_tag="ACHUPM_000778"
     CDS             complement(7537..7863)
                     /gene="trpR"
                     /locus_tag="ACHUPM_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR0868646.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7953..9890)
                     /gene="sltY"
                     /locus_tag="ACHUPM_000779"
     CDS             complement(7953..9890)
                     /gene="sltY"
                     /locus_tag="ACHUPM_000779"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR0868647.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10101..11768
                     /gene="ettA"
                     /locus_tag="ACHUPM_000780"
     CDS             10101..11768
                     /gene="ettA"
                     /locus_tag="ACHUPM_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR0868648.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11929..12036)
                     /gene="nadS"
                     /locus_tag="ACHUPM_000781"
                     /pseudo
     CDS             complement(11929..12036)
                     /gene="nadS"
                     /locus_tag="ACHUPM_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(12075..13307)
                     /gene="nadR"
                     /locus_tag="ACHUPM_000782"
     CDS             complement(12075..13307)
                     /gene="nadR"
                     /locus_tag="ACHUPM_000782"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR0868649.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13328..14710)
                     /gene="radA"
                     /locus_tag="ACHUPM_000783"
     CDS             complement(13328..14710)
                     /gene="radA"
                     /locus_tag="ACHUPM_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR0868650.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14759..15727)
                     /gene="serB"
                     /locus_tag="ACHUPM_000784"
     CDS             complement(14759..15727)
                     /gene="serB"
                     /locus_tag="ACHUPM_000784"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR0868651.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15833..16477
                     /locus_tag="ACHUPM_000785"
     CDS             15833..16477
                     /locus_tag="ACHUPM_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR0868652.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16505..17521
                     /gene="lplA"
                     /locus_tag="ACHUPM_000786"
     CDS             16505..17521
                     /gene="lplA"
                     /locus_tag="ACHUPM_000786"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFR0868653.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            17553..17816
                     /locus_tag="ACHUPM_000787"
                     /pseudo
     CDS             17553..17816
                     /locus_tag="ACHUPM_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(17977..18696)
                     /gene="deoD"
                     /locus_tag="ACHUPM_000788"
     CDS             complement(17977..18696)
                     /gene="deoD"
                     /locus_tag="ACHUPM_000788"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR0868654.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18776..19999)
                     /gene="deoB"
                     /locus_tag="ACHUPM_000789"
     CDS             complement(18776..19999)
                     /gene="deoB"
                     /locus_tag="ACHUPM_000789"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR0868655.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20051..21373)
                     /gene="deoA"
                     /locus_tag="ACHUPM_000790"
     CDS             complement(20051..21373)
                     /gene="deoA"
                     /locus_tag="ACHUPM_000790"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR0868656.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21500..22279)
                     /gene="deoC"
                     /locus_tag="ACHUPM_000791"
     CDS             complement(21500..22279)
                     /gene="deoC"
                     /locus_tag="ACHUPM_000791"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR0868657.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22537..24087
                     /locus_tag="ACHUPM_000792"
     CDS             22537..24087
                     /locus_tag="ACHUPM_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR0868658.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            24059..24922
                     /locus_tag="ACHUPM_000793"
     CDS             24059..24922
                     /locus_tag="ACHUPM_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR0868659.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(25438..26220)
                     /locus_tag="ACHUPM_000794"
     CDS             complement(25438..26220)
                     /locus_tag="ACHUPM_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0868660.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(26217..27290)
                     /locus_tag="ACHUPM_000795"
     CDS             complement(26217..27290)
                     /locus_tag="ACHUPM_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR0868661.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(27412..27573)
                     /locus_tag="ACHUPM_000796"
     CDS             complement(27412..27573)
                     /locus_tag="ACHUPM_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR0868662.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27700..28305)
                     /gene="osmY"
                     /locus_tag="ACHUPM_000797"
     CDS             complement(27700..28305)
                     /gene="osmY"
                     /locus_tag="ACHUPM_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR0868663.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28698..30287)
                     /gene="prfC"
                     /locus_tag="ACHUPM_000798"
     CDS             complement(28698..30287)
                     /gene="prfC"
                     /locus_tag="ACHUPM_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR0868664.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(30378..31055)
                     /gene="yjjG"
                     /locus_tag="ACHUPM_000799"
     CDS             complement(30378..31055)
                     /gene="yjjG"
                     /locus_tag="ACHUPM_000799"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR0868665.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(31070..31516)
                     /gene="rimI"
                     /locus_tag="ACHUPM_000800"
     CDS             complement(31070..31516)
                     /gene="rimI"
                     /locus_tag="ACHUPM_000800"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0868666.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31485..31898)
                     /gene="holD"
                     /locus_tag="ACHUPM_000801"
     CDS             complement(31485..31898)
                     /gene="holD"
                     /locus_tag="ACHUPM_000801"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR0868667.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            32001..33032
                     /gene="rsmC"
                     /locus_tag="ACHUPM_000802"
     CDS             32001..33032
                     /gene="rsmC"
                     /locus_tag="ACHUPM_000802"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR0868668.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT         7848 a         9159 c         8513 g         7776 t
ORIGIN      
        1 gattattaaa ataatgtgac gaaagctagc atttagatac gatgatttca tcaaactgtt
       61 aacgtgctac aattgaactt gatatatgtc aacgaagcgt agttttattg ggtgtccggc
      121 ccctcttagc ctgttatgtt gctgttaaaa tggttaggat gacagccgtt tttgacactg
      181 tcgggtcctg agggaaagta cccacgacca agctaatgat gttgttgacg ttgatggaaa
      241 gtgcatcaag aacgcaatta cgtactttag tcatgttacg ccgatcatgt taatttgcag
      301 catgcatcag gcaggtcagg gacttttgta cttcctgttt cgatttagtt ggcaatttag
      361 gtagcaaaca tgcagacccc gcacattctt atcgttgaag acgagttggt aacacgcaac
      421 acgttgaaaa gtattttcga agcggaaggc tatgatgttt tcgaagcgac agatggcgcg
      481 gaaatgcatc agatcctctc tgaatatgac atcaacctgg tgatcatgga tatcaatctg
      541 ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg agcaggcgaa tgttgcgttg
      601 atgttcctga ctggccgtga caacgaagtc gataaaattc tcggcctcga aatcggtgca
      661 gatgactaca tcaccaaacc gttcaacccg cgtgaactga cgattcgtgc acgcaacctg
      721 ctgtcccgta ccatgaatct gggtactgtc agcgaagaac gtcgtagcgt tgaaagctac
      781 aagttcaatg gttgggaact ggacatcaac agccgttcgt tgatcggccc tgatggcgag
      841 cagtacaagc tgccgcgcag cgagttccgc gccatgcttc acttctgtga aaacccaggc
      901 aaaattcagt cccgtgctga actgctgaag aaaatgaccg gccgtgagct gaaaccacac
      961 gaccgtactg tagacgtgac gatccgccgt attcgtaaac atttcgaatc tacgccggat
     1021 acgccggaaa tcatcgccac catccacggt gaaggttatc gcttctgtgg tgatctggaa
     1081 gattaatcgg ctttaccacc gtcaaaaaaa acggcgcttt ttagcgccgt ttttattttt
     1141 caaccttatt tccagatacg taattcatcg tccgttgtaa cttctttact ggctttcatt
     1201 ttcggcagtg aaaacgcata ccagtcgata ttacgagtca caaacatcat gccggccagc
     1261 gccaccacca gcacactggt tcccaacaac agcgcgctat cgacagagtt gagcagtccc
     1321 cacatcactc catccagcaa caacagcgcg agggtaaaca acatgctatt gcgccagcct
     1381 ttcaataccg cctgcaaata aataccgttc attaacgccc caatcagact ggcgattatc
     1441 catgccacgg taaaaccgat atgttcagaa agcgccagca agagcaaata aaacatcacc
     1501 aatgaaagcc ccaccagcaa atattgcatt gggtgtaaac gttgcgcggt gagcgtttca
     1561 aaaacaaaga acgccataaa agtcagtgca atcagcagaa tggcgtactt agtcgcccgg
     1621 tccgttaatt ggtattgatc ggctggcgtc gtcactgcga cgctaaacgc gaggaagttt
     1681 tcccagccgg tatcattgcc tgaagcaaaa cgctcaccga gattattagc aaaccagctg
     1741 ctttgccatt gcgcctgaaa acctgactcg ctaacttccc gtttggctgg caaaaaatca
     1801 cctaaaaaac tgggatgcgg ccagttgctg gttaaggtca tttcgctatt acgcccggca
     1861 ggcaccacag aaagatcgcc ggtaccgctt aaattcaggg ccatattcag cttcaggttc
     1921 tgcttccgcc agtccccttc gggtaaaggg atatgcacgc cctgcccgcc ttgctctaac
     1981 ccggtgccgg gttcaatggt cagcgccgtt ccgttaactt caggcgcttt caccacacca
     2041 ataccacgcg catccccgac gctaatcaca ataaatggct tgccaaaggt gatatttggc
     2101 gcgtcgagtt cgctaagacg cgaaacatcg aaatcggctt ttagcgttaa atcactgtgc
     2161 cagacctgac cggtataaat ccctatcttg cgttcttcca cgttctgatt gccatcaacc
     2221 atcaatgact caggtaacca aaaatggata aaacttcgtt tccgctccac ggttttatcc
     2281 tcttcctgta ccgtataaag ctcggtcaca ggaatagcga taagcggccc aacaagtttt
     2341 tgcggcccgc tggtactttg gcgaatcgca tcttccacat cgctacggta atcggcacgt
     2401 tcgacaatca cctgccgaat cagcattatc ggaatcaaca acagcaatac tgcaccaaac
     2461 agggtggtca ttttccagaa cagggaggat ttcaacatag tcttctcctt tgcaatggca
     2521 gcaggatacg aaggctatgt gggaagaatt tgaagctatg tgaagtgacg gtgaagtcga
     2581 agcgaggcca gcacgccacc ttcctgcacg ttgcgcagcg tgacttcgcc gttaaacaaa
     2641 cgggcgacct cactgacgaa cgccaacccc agaccgctgc ttttttgccc atttgcacgc
     2701 ggcaaagagt aaaagcgttc aaaaatacgt gacagcgcgt agtcaggaat accactaccg
     2761 gtatccagca ccttaagcgt gacgtgttcc tgatccactt cggcgcttag cgttatacga
     2821 ccgctctcgg gggtaaaatc gatggcgtta tccagtaaat tccccagcgc ctgatccagt
     2881 aacgccggtt cagcagcaac attaacctcg gtgggcgtaa catgtagagt gatgtttttt
     2941 tctgccaact gcacggtgcg cgcttcgctg acgcggcgga ataatgccgc cacatcaaca
     3001 gcagtcaaaa cgacttcctg acgattctcc agtcttgcct ggcgtagtaa cgtttccacc
     3061 agtgcctgca ttcgcgcatt ttgcgtcaga atgttgtcag taaaacgagc cactacttca
     3121 ggcggcggac cttcgcgtaa aatttccgcc gcgccacgaa tcgccgccag tgggcttttt
     3181 agctcatggg ttaacgcata aacatactgc tcaatatagt ttttcccttc cagcttcacg
     3241 cgcatacttt ccagcgcctg cgcgagttta cgcagctcgc tactaccgag atcgggaaga
     3301 ggaacgggct tattgtcagt gacggaatca gcatagcgag tgagccgggc aatggagcgg
     3361 ttgatccacc aaaccatgcc tgcgccaatc accagtgcga tccccaacaa aatggcgctg
     3421 gcccataaaa ttcgccgctc gctgcgttta atgaccggag ccatcgccgc gttcggtttg
     3481 cctacgctca aaacgccaat aagccgcgag ccgtccataa tcggcgcggc aacatacatc
     3541 accgaacttt cgggatcggc aggattttgc aacgtgctac gcgcaccata ctgaccacgc
     3601 aacgttagcc agacgtcatt ccagcgcgaa taatcctgcc caacggcttt atttgccgaa
     3661 tcgaacaata ctttgccctg cgcatcggtc atatagacat gatattcatt gcgcactttg
     3721 ttaatgccac cgatattggc gcgaaacggg cgatgttgta gctgattaaa cgcctgagcc
     3781 agttgcccat gcgttgggtc cccagagagc aaatcgggac gcgccagctc cgccagcaac
     3841 gttgcggtat cgattaacgt cccctccgtt gctcttcgca cgcccggttt aacttctttg
     3901 acaaaaatag ccagtacgaa ccaggccgcc accgccacca gtaaaaaata gcccagcagc
     3961 aaccgcatgc cgatacgcat tacaggcccc tcaggctata tcccatgccg cgatgagtat
     4021 taatcggaga aagatcgggg ttgatggcgc gcagcttggc acgcaacgtt ttaatgtggg
     4081 tatcgacggt gcgatcgtag gtgtcctgcg catcttccca cacgctatcc attagttgct
     4141 ggcgggacca gacgcggcct ggcgacttga gtaacgtctt cagcaataaa aactcatacc
     4201 gcgtcagcgt taatggcgtg tcaaaccagc tgatctgcgc cgcgggttca ttcaattcaa
     4261 aatgaccaat acggatgacg ggagacggcg acgagaactt cttcacccga cgcagtaagg
     4321 tgcgcaccct ggcgcacact tcgcggggtg aaaacggttt agccacatag tcgtcagcgc
     4381 caatttccag cccaagcagg cgatcgacct cttcacttcg ggccgtcagg aacagtacag
     4441 gtaacgccgg atggagcgcc agtaactggc ggcacaattc aaagccgctg atatcaggca
     4501 aaccaacatc gagaatcatg acgtcgggaa cctgctggcg agctttatcc agcaccggca
     4561 agcctcgctc aaagacctcg acggcaaaac cttcctgctg caacatgtag accagcgtgt
     4621 cggctatccc ttgctcatct tccactaacc agaccgtttc ccgttgcata aatcgcctct
     4681 gttattgccg ccacggcatg acaggaaccg cgctaatcgc gtttttcggc gaaccttcta
     4741 caactttgtc ggagtaagcc agataagcga gcgcgttgcg tttggcgtca taaaagcgca
     4801 cgacctgtaa cgacttaaag accagggacg tgcgtttttt gaatactacc tcgccctgag
     4861 ctttgccgtt tttaatacga tccgacagtt caatcggccc gacttgctga caagaaatag
     4921 ccgcatcgga ggtatcttcc gccagaccca atcccccttt aataccacct gttttcgccc
     4981 ggctcacata acaggtgaca tttttcacat cgggatcgtc aaaggcttcc acaacaattt
     5041 tgtgatccgg gccgatcatt ttaaatacgg tgtcgaccga accaatctct tctgcatgag
     5101 ccaatggccc cagcattatc aggcttaaag acaggatcaa atgcttgtat ttcatattgt
     5161 taccattttt atctattaca cgagcaatta gttcgtcacg gtaaataaca tattgattaa
     5221 gaacaaaatc tcaatacttt tatttccgtc aagccctaaa acatactcta ctaagaaaaa
     5281 aacactgaat gctaaaacag caaaaaatgc tattatccaa ttacctgatg tcaggtgctc
     5341 gttgttgaaa ggatgaggat attttatgga tcaggccggc attattcgcg accttttaat
     5401 ctggctggaa ggtcatctgg atcagcccct gtcgctcgac aatgtagcgg cgaaagcagg
     5461 ttattccaag tggcacttac agagaatgtt taaagatgtc actggccatg ctattggcgc
     5521 gtatattcgt gctcgtcgtt tgtcgaaatc ggcggtcgca ctacgcctga ctgcgcgtcc
     5581 gattctggac atcgcgctgc aataccgctt cgactctcaa cagacattta cccgcgcatt
     5641 caagaagcag tttgcccaga ctcctgcact ttaccgccgt tctcctgaat ggagcgcctt
     5701 tggtattcgc ccgccgctac gtctgggtga attcacaatg ccagagcaca aatttgtcac
     5761 cctggaagat acgccgctga ttggtgttac ccagagctac tcctgttcgc tggagcaaat
     5821 ctctgatttc cgccatgaaa tgcgttatca gttctggcac gattttctcg gcaacgcgcc
     5881 gaccattccg ccagtgctct acggcctgaa tgaaacgcgt ccgagtcagg ataaagacga
     5941 cgaacaagag gtattctata ccaccgcgtt agcccaggat caggcagatg gctatgtact
     6001 gacggggcat ccggtgatgc tgcagggcgg cgaatatgtg atgtttacct atgaaggtct
     6061 gggaaccggc gtgcaggagt ttatcctgac ggtatacgga acgtgcatgc caatgctcaa
     6121 cctgacgcgc cgtaaaggtc aggatattga gcgatactac ccggcagaag acgccaaagc
     6181 gggagatcgc ccaattaatc tgcgctgtga actgctgatt cctatccgcc gttaacgctg
     6241 cagctcatct aatgcagggg cgtccagatg cgagatgtcc cctgcggttt caacgaccca
     6301 gccagacgcc agccacaggc tttcctgata atccacgcgc gaaatagaac agttacgcag
     6361 acgtaagcgg cgctctgccc atgctggtaa tccgagaatc gtactcacca gacatcccag
     6421 tgcaatacca tggctcacca gcagcggtcg gcttccctgc ggtaagtccc ggcaagattc
     6481 cagtgccgca ttgacgcgat cgctgagctc ctgcattgac tcgccttcag gaatacgccc
     6541 gtcaacggtg ccattgacca gctgccgacg ccagttctct tcttcttcgg tcagagaatc
     6601 gatatgtctt ttctccagca cacccatgtt taattcacgc aggcgagaat caaagatgat
     6661 gtcacagccg caggcctggg cgatgatttc cgccgtacgc cgggtgcgtc ctaaatcgct
     6721 actgataata tgcgtaatgc caagctcttt ggcacgggtt gccacctgca tcgcctgttg
     6781 ctcacctttg gcggtcagcg ggctgtcaga ctggccctga atacgtcgct cggcgttcca
     6841 ctgcgtttca ccgtggcgga ctaggtatac ctgtaacatg ctttttttcc gtatactgct
     6901 gtaacgttaa ttttctgagt aatgctgatt atgcaccaag ttatctgtgc gaccaccaat
     6961 cccgctaaaa ttcaggccat tctgcaggca tttcacgaga tcttcggcga aggatcctgc
     7021 catattgcat ccgttgccgt cgagagcggt gtaccggaac agccctttgg cagtgaggaa
     7081 acgcgcgctg gcgcacgaaa tcgggtagca aatgcccgcc gtttacttcc agaggctgat
     7141 ttttgggtgg cgattgaagc tggcatcgat ggtgacagca ctttcagttg ggtggtgatt
     7201 gaaaacacca gccagcgcgg cgaggcgcgt tcggctactt tgccgttacc tgcggtgatt
     7261 ctgcagaaag tgcgtgaagg tgaggcgctt ggtcccgtga tgtcgcgtta taccggtatt
     7321 gatgagattg gtcgtaagga aggggcgatc ggcgtgttta ccgccgggaa actcactcgc
     7381 gccagcgtct atcaccaggc ggtgatcctg gcgctgagtc cgtttcacaa tgccgtgtat
     7441 caggcattgt aggacggata aggcgttcac gccgcatccg gcattcggtg cacgatgcct
     7501 gatgcgacgc tgccgcgtct tatcaggcct acaaaatcaa tcgcttttca gcaacacctc
     7561 ttccagccac tggcgcagct ccacgggcgc ggctttcagg ctgttagatc cacgcgtaat
     7621 cgtcgcgatg cccgcgccga gttcattttt taactcacgc tgactcattt cgccacgcaa
     7681 cagctcttcg acaatacgca cgcgagtccc caacgcttcg cgctcatctg gcgtcagcat
     7741 caggtttaac aacggtaaat ggagatcgtt ttggtaggca ttcttaagca ggtcgacaaa
     7801 acgtaaccac tcctggtgac gctgttctgc catcgctgct gaatagggtg attgttgggc
     7861 cataatatgt cgccattgtt agcgggggaa gcaaaatgcc tcccccggtt gtactcgcta
     7921 aagagtacga tagcatatca taaacgtgcg gatcagtaac gacgtcccca ttccgtggcg
     7981 ctcatcaacg tcggtttatc ccccatgaaa taacggtagt aagcgtcata agccagcacg
     8041 ttcttcacat aaccgcgcgt ctcggagaac ggaatactct cgacaaatgc cacagcgtcg
     8101 atacgcccgg cgctgttgcc aagccaggtt cgcacccgtc caggtccggc gttataagct
     8161 gctgaggaga aaatacggtt attgccaaac tgctgataaa catattgcag gtaactggtg
     8221 ccaatgttga tattcgtttc cggatccagc aattgcccag ggctgctata accaggaata
     8281 gagaacatct tcaccgtatg ggtcgctgta ccaggcataa tctgcatcag gccgctggcc
     8341 cctaccggtg atttcacttt cggattccag gcgctctcct gacgggcaat cgccatcgca
     8401 tagctttgcg ggatctcttt cccactggtg taacgtttga aaagatcgtt gtaagccagc
     8461 gggaatcgct cttccagatg atcccaaagc ttcccggcga tcgttgcctg aacgctaaga
     8521 tcccaccatt ggttattgaa agcataccga gccagttgag cctgctctgt ttttgacttg
     8581 ctcttcacca gattggccca ctcgctacgc gcggtgttat cgagattcca gtacatcaac
     8641 tcgcgcacgc gcgccatctc cggcccctga gtcagggcgc tgtcaacatt ctgcggcgct
     8701 ttatcaatct tcagctcata ctcttctccg atgcgttgtg ctgcaaccat cgggtagaaa
     8761 ccacgctgtt gcatgagttg atgcaaaatc tctttcgctt cagcttcacg tccgcgttcc
     8821 agcaataaat ccacctgcca gtaacgccat tcatctttct cttttgcttc catcggcaga
     8881 cgcgccagcc aggtattcag gccgcgacga tcgccggtgc caagtgccat tcgcacacgg
     8941 cgttcaatca acgacgtgga ttgcgagcgc ataatgacgt catcgcgcca tttcgcctgc
     9001 tcgtcggtga catcgttgcc catcaaacgc caggcgacga tatcgcgcag ctcctgaatc
     9061 tgatcttcat taagctgctg cacctgggca agcgacggga tcatcagccg tgcattttca
     9121 gcatcctgcc gcgccacact ggcaaacgcc accgctgcca tttgacgggt aaaatcggtc
     9181 gctccggttg tacgcgcgaa ggtcagtacc gtattagggt tgttcgccag tgaaatgatt
     9241 gccgaggcga tagtctggta atcggcaggc atctgccctg ccagcactgt taccaggcca
     9301 gtgttacccg ctttcatcgc cagacggata cgctctaaat acgccagcgg atcttgttta
     9361 cctgacgcac gccagacgct aaataacttg tcacaggcgt taggctggct cttgccggtt
     9421 agccacagct ctttcgcccc ttgccaggct tcttcactct gcccggtgtt ccatttcgca
     9481 tagtagtaat tacattgcgc ttcggtagtt ccgggctttt ccgggctaaa ggctaacaag
     9541 ccacgccagt cttcacgccg cgccagttca ttgacgaaac gagattgcag cgtgcgagcg
     9601 ggaggaagcg tggggttagc gcgaacaaag ttagtgaccg tcaccgccgg ttgattcatc
     9661 agatcatcgg tgatctggcg gtattccagg tagggataaa gcggataatc cttcagtcca
     9721 ggcatcattt gttcgaccac atccatttgt cgattatccc aggcctgctt gatttgcgcg
     9781 taacggctac gctgctcatc cagtgagtcg gctcgcgcca cgctgctgac cgtcagcaga
     9841 cagacaccgg cagccaacag ccgccaggta acttgtttgg ctttttccac aagcgcatcc
     9901 tctaagtgta aatacatcaa tgcagcgtca tgccgcgtaa tagatttatg ctaaccagtc
     9961 attgccgttt acgccacgtt acggacactt ttttactttt actgcgaagg tgatcgacca
    10021 actgggatta ggcggggaaa aaggctacac ttcgcctttg aaaactcatc atcacgataa
    10081 acatagaggc gaagtccaac gtggctcaat tcgtttatac catgcatcgt gtcggcaaag
    10141 ttgttccgcc gaaacgtcat attttgaaaa acatctctct gagtttcttc cctggggcaa
    10201 aaattggtgt cctgggtctg aacggcgcgg gtaagtctac cctgctgcgc attatggcgg
    10261 gcattgataa agacatcgaa ggtgaagcgc gtccgcagcc agacatcaag attggttacc
    10321 tgccgcagga accgcagctg aacccggaac acaccgtgcg tgagtccatt gaagaagcgg
    10381 tttctgaagt ggttaacgcc ctgaaacgcc tggatgaagt gtatgcgctg tacgccgatc
    10441 cggatgccga ttttgacaag ctggccgctg aacaaggccg tctggaagag atcattcagg
    10501 ctcacgacgg tcataacctg aacgtacagc tggagcgtgc ggcggatgcg ctacgtctgc
    10561 cggactggga cgcgaaaatc gctaacctct ccggtggtga gcgtcgtcgc gtagcgttgt
    10621 gccgcctgct gctggaaaaa ccagacatgc tgctgctcga cgaaccgacc aaccacctgg
    10681 atgccgaatc cgtggcctgg ctggaacgct tcctgcacga cttcgagggc accgtggtgg
    10741 cgattaccca cgaccgttac ttcctcgata acgttgcagg ctggatcctc gaacttgacc
    10801 gcggtgaagg tattccgtgg gaaggcaact actcctcctg gctggagcag aaagatcagc
    10861 gcctggcgca ggaagcttca caagaagcgg cgcgtcgtaa gtcgatcgag aaagagctgg
    10921 agtgggtacg tcagggaact aaaggccgcc agtcgaaagg taaagcacgt ctggcacgct
    10981 ttgaagagct gaacagcacc gaatatcaga aacgtaacga aaccaacgaa ctgtttattc
    11041 cacctggacc gcgtctgggc gataaagtgc tggaagtcag caacctgcgt aaatcctacg
    11101 gtgatcgcct gctgattgat gacctgagct tctcgatccc gaaaggggca atcgtcggga
    11161 tcatcggtcc gaacggcgcg ggtaaatcga ccctgttccg tatgatctct ggtcaggaac
    11221 agccggacag cggcaccatc actttaggtg aaacggtgaa actggcgtcg gttgatcagt
    11281 tccgtgactc aatggataac agcaaaaccg tttgggaaga agtttccggc gggctggata
    11341 ttatgaagat cggcaacacc gagatgccaa gccgcgccta cgttggccgc tttaacttta
    11401 aaggggttga tcagggtaaa cgcgttggtg aactttccgg tggtgagcgc ggtcgtctgc
    11461 atctggcgaa gctgctgcag gttggcggca acatgctgct gctcgacgaa ccaaccaacg
    11521 acctggatat cgaaaccctg cgcgcgctgg aaaacgccct gctggagttc ccgggctgtg
    11581 cgatggttat ctcgcacgac cgttggttcc tcgaccgtat cgccacgcac atcctggact
    11641 accaggatga aggtaaagtt gagttcttcg aaggtaactt tactgagtac gaagagtaca
    11701 agaaacgcac gctgggcgca gacgcactgg agccgaagcg tatcaagtac aagcgtattg
    11761 cgaagtaatg cgtaaaatgc cggatgcggc gtgaacgcct tatccggcct acaaaccaag
    11821 aaatttcaat acgttgtgaa tctccaggcc tgataagacg cggtaagcgt cgcatcaggc
    11881 attgagcacc gaatgccgga tacggcgtga acgccttttc cggcctacaa cagccttagt
    11941 atccgcaatt tcgtagcggg tcacccgtgc aggttttttc aaaccttgtt taatctcgac
    12001 agcctcttcc agagaggttt tcaactcatc aaaaaactta ttttatacct cgcctttgag
    12061 ccgtttcatc gcggttatct ctgctccccc atcatctccc gcaccagctc cacgcagcgc
    12121 aggaaacggc tgtcataatc gtcctcttca acccgcacga attcgatatt gttctcttcc
    12181 agcatctcca ccagcaagtt ctggaactct ttgcgatcca ccgaactgcc gaggctgcgt
    12241 aaaccatccg ccacccacgg cgtgttgttc tccagcagga tcaccagatc gaaacggtat
    12301 tcatcaatca acgcctgtac gaacggatgc tcacgccctt cgtacttttt gcagaacgcc
    12361 tgggtagtga caaaatcagt gtcaataaac gccactttat tggcatattt cactgcaaaa
    12421 tcaatgtatt gtgcgtggcc cagcgcgatt ttatcgtaat cggaatactg caatgcgatc
    12481 tcatcaccgc cgaggtgtga aaagacataa tcgcgaccat attcccacgc actggtggta
    12541 ttgaagatat tggcaagttt gtttaccagg gtggatttac cgctcgactc gccaccaagg
    12601 atcgccacgg tacgtacaaa gaacggcttc acttccgtag gaatatattc ccagtagcgg
    12661 aacgggtttt cgcggatctg cgcaccgctg atactcataa aggtacgttt cggatcgacc
    12721 agcaccgtat cgatccccag atgttccata tactgtggcg catcggcttc ttccgaggtg
    12781 tagatcaggt ccggctgaat ccccttttca gccataaact ttttgatgcc gttgctccac
    12841 acatcccagc cgtgcggata cggctccatg ccctcttcgt tgaaagcatg aatgcgaata
    12901 tttttctgat atttaaaagt ttgtaataac cagcgcagac gatccggcac cgtgggctgc
    12961 tgcgacatgg cgctgtcttc aaacaacgcg cggtcgcggg tatcgtcaaa acccataatg
    13021 atatgcagct cgtcaacctg gctacaggcg cgctggataa ggtagatatg tccggtatgc
    13081 agcgggtaga acttaccgaa tacgacacca atggttttct tctgtcgggg aaattcaagc
    13141 cccaaaaaac ggtgcaacgc ctccagcttt tgcgcgctgg ggcttttaat tttggcattc
    13201 agtaactggc ttaaataccc tttggtcata ccgctggcat cagctacctg ctgtagcgtg
    13261 cagccctgtt gcttgatggc agttttcagg taatcaaatg acgacatatc tccctccgta
    13321 tctctcatta taagtcgtcg aacacgctaa gcgcgtcgga gagtttttta acgccaaaaa
    13381 tctgcatccc ttccggcgct tttttcggta cgttagccgc cggaacaatc gcccggcgaa
    13441 aaccgtgttt cgccgcttct gaaattcgtt cctgaccgct gggcaccggg cggatctccc
    13501 ctgccagccc gacttcacca aacaccacca gatcctgtgg cagcggtctg tcacgcaggc
    13561 tggaaaccat cgccagcagt aacgctaaat cggcactggt ttcggttacc ttcacgccgc
    13621 cgaccacgtt cacaaataca tcctgatcgg ccatttgcag accaccgtga cggtgcaaga
    13681 cagccagcag gattgccaga cggttttgtt ccagccccac tgccacgcgg cgcggattcg
    13741 ccatcatcga gtgatcgacc agcgcctgaa tctccaccag caacggacgc gttccttccc
    13801 acaccaccat cacggagctg ccggaggtca cttcatctcc gcgacttaag aaaattgccg
    13861 aagggttgct gacttcacgc agcccctgtt cggtcatcgc gaagacgccc agctcattca
    13921 ccgcgccgaa gcggttttta tggctgcgca aggtgcgaaa acgggagtcg gcatcaccat
    13981 ccagaagcac cgaacagtcg atacagtgtt ccagcacttt agggccagcc agcgagccat
    14041 ctttggttac gtgcccaacc atgacaatcg ccacaccgcg cgttttggcg aagcgcgtca
    14101 gataagccgc cgtttcacgc acctgcgcca cgctaccagg cgatgattgt acatccgcca
    14161 tatgcatcac ctggatcgag tcaattacca tcagtttcgg ttgctcttct tcggcaatca
    14221 ggcagatctg ttcgatgctg gtttccgaca acatattgag attgtcagtc ggcaggccaa
    14281 ggcgatgagc gcgcattgcc acctgttgca gcgactcttc gccagtgaca tacagcgttt
    14341 tcatctgctg ggccagtttg cacagcgttt gcagtagcag cgtggatttc cccgcacctg
    14401 ggttaccgcc aatcagaatg gcgcttcccg gcaccacgcc gccgcctagt acgcggtcga
    14461 actctttaaa tccggtggaa aaacgcggca gctcttcaag gctgatatcg gagagtttct
    14521 ggacttttgc caccccggcg ctaccggcat agccgctgag acgctcgtta cgcgccaccg
    14581 ttggcgacgc agcaagacgc acctcggtga tggtgttcca ggcatgacag gcactgcact
    14641 gcccctgcca gcgcggataa tcggccccgc attcattaca aacaaaggcg cgttttggag
    14701 cttttgccac ggtgtacctc gttaatgctg tgccgcccgc aggatggcgg gcgagcaatt
    14761 acttctgatt caggctgcca gagaggatgc agaatacccc catcaggtca gcgtgacgga
    14821 tggtgacttc cgccttttca ttcacttttg gcttggcatg gtaggcaatc cccagccctg
    14881 ctgctttgat catcggcagg tcattagctc catcgccaat cgccacggtc tgcgccagcg
    14941 ggatttcata ctcctgcgcg aggcgagtca aagttttcgc tttgtactgc gcgtctacga
    15001 tgtcgccaat cacattgccg gtaaatttac cgtccatgat ctccagttca ttggctacca
    15061 cggcggtcag gcgcagcttg tcgcgcagat attcagcaaa gaaagtaaag ccgccggagg
    15121 caatcgccac tttccagccc agcgtttcca gcttgagcac cagttgcgtt aagcctggca
    15181 tcagcggcag attttcacgc acctgttgca gaatattggc gtcagcgcct ttcagcgtcg
    15241 ccacacggct gcgcaggctg gcggtaaaat cgagttcgcc gcgcatcgcc cgttcggtta
    15301 cttccgccac catctcgccc gttccggcca gtttggcaat ttcatcaata cattcaatct
    15361 ggatggcggt ggaatccatg tccatcacca gcaaacccgg cgtgcgcagg tgcgggattt
    15421 tccccagcgg ggcgacatcc agttgcgctt catgggccag gcgcgtagcc cgtgcggtga
    15481 gtgaacctgc cagacgaatc acctgataat cttccacgca ccaggcggca acaatcacca
    15541 tcgccgcacc cagtttgctc tggtattggg tcagacgctg tttatccagc ccacgaccat
    15601 acagcagcca gccgctacga cctgcgtggt aatccagtgg catcacttca tcaccactta
    15661 atgaaagagg cagacccggc cataaagaga catcttcagg caggtcgcac caggtaatgt
    15721 taggcattaa ggctcctgta aaatcgttcg aagcagggaa aataacgcat gaggctacct
    15781 tgtatccatt gcttctggca acattaagtc tcaaattttc aaagggtgga agatggctcg
    15841 cacaaaactg aaattccggc tgcatcgggc agtgattgtc ctgttctgtc ttgccttgtt
    15901 agtggcactg atgcaggggg cgtcatggtt tagtcaaaac caccagcgac agcgtaatcc
    15961 acagctggaa gaactggccc gcaccctggc gcgtcaggtg acgctgaacg ttgcaccgct
    16021 gatgcgtacc gactcaccag atgaaaaacg cattcaggcg atcctcgatc agttaacgga
    16081 tgaaagccgc atcctcgacg cgggtgtgta tgacgaacaa ggcgatctta tcgcccgttc
    16141 aggcgaaagc gtcgaagtgc gcgaccggct agcgctcgac ggtaaaaaag caggcggcta
    16201 ttttaaccag cagattgtcg agccaattgc gggtaaaaac ggaccgctcg gctatctgcg
    16261 cctgacactc gacacccata cgctcgccac cgaagcccaa caggtggata acaccactaa
    16321 cattttacgc ctgatgttgc tgctctcact ggcaatcggc gtagtgctga cccgcacgct
    16381 gctacagggt aaacgcaccc gctggcagca atcgcccttc ctgttaaccg ccagcaaacc
    16441 ggtgccggaa gaggaagaaa gcgagaaaaa agagtgaccc attactacaa gaaaggaaat
    16501 cgttatgtcc acattacgcc tgctcatctc tgactcttac gacccgtggt ttaacctggc
    16561 ggtggaagag tgtatttttc gccaaatgcc cgccacgcag cgcgttctgt ttctctggcg
    16621 caatgccgac acggtagtga ttggtcgcgc gcagaacccg tggaaagagt gtaatacccg
    16681 gcggatggaa gaagataacg tccgcctggc gcggcgcagt agcggtggcg gcgcggtgtt
    16741 ccacgatctc ggcaatacct gctttacctt tatggctggc aagccggagt acgataaaac
    16801 catctctacg tcgattgtgc tcaatgcgct gaacgcgctc ggcgtcagcg ccgaagcgtc
    16861 cggacgtaac gatctggtgg tgaaaacagt cgaaggcgac cgcaaagtct caggctcggc
    16921 ctatcgcgaa accaaagatc gtggcttcca ccacggcact ttgctgctca atgccgacct
    16981 tagccgcctg gcaaactatc tcaatccgga taaaaagaaa ctggcggcga aaggcattac
    17041 ctcagtgcgt tcccgcgtga ccaacctcac cgagctgctg ccggggatca cccatgagca
    17101 ggtttgcgag gccataacca aggccttttt cgcccattat ggcgagcgtg tagaagcgga
    17161 aatcatctcc ccggacaaaa cgccagactt gccaaacttc gccgaaacct ttgcccgtca
    17221 gagtagctgg gaatggaact tcggtcaggc tccggcattc tcgcatctgc tggatgaacg
    17281 ctttagctgg ggcggcgtgg aactgcattt cgacgttgaa aaaggccata tcacccgcgc
    17341 ccaggtgttt accgacagcc tcaaccccgc gccgctggaa gccctcgccg ggcgactgca
    17401 aggctgcctg taccgcgcgg atatgctgca acaagagtgc gaagcgctgt tggttgactt
    17461 cccggaccag gaaaaagagc tacgggagtt gtcgacgtgg atagcggggg cggtaaggta
    17521 actcgctgat taaaatacca aaggaaggga atatgatcga tctggaaaac caggagcggg
    17581 agattatcaa tttaatgtta agccagcgta tttcctggct agccgccgtg cgtatcaggc
    17641 ataaattatc acttgctgaa gtaagtaaaa tgttaggtat ctcgattaat tctctaaaat
    17701 agatcgaaaa gacggaacgg cttagctcaa acatcaaaag caaaatggga gaaatttatg
    17761 gttgtccacc agagttgctg atatgccctt cctggatgac ggctgaacat aaatgaatag
    17821 attcgtgaat agatacttga atgtatttta ttttttatcc atatgaaaaa taatagtaaa
    17881 tctataaatc agttgctatt gatgaataga tactaaaaag ccggagcagt cccccactcc
    17941 ggcttaacac gacaatcgcc tttcagcgaa acacaattac tctttatcgc ccagcagaac
    18001 ggattccagt gcgattttga tcatgtcgtt gaaggtggtc tgacgctcag cggcagtggt
    18061 ctgctcgtga gtgcggatgt ggtcagacac agtgcagatg gtcagggctt tcgcgccaaa
    18121 ttctgcagcg acgccgtaga taccagccgc ttccatttcc acgccgagga tgccgtattt
    18181 ttccatcacg tcgaacattt cgccgtccgg agagtagaac aggtcagcgg agaacaggtt
    18241 accaacgcga gcatcaatgc ccagtgcttt agccgcgtct accgcgttac gcaccatgtc
    18301 aaagtcagcg atagcggcaa agtcatgatc tttaaaacgg atgcggttaa ctttggaatc
    18361 ggtgcaggca cccataccga taacgacgtc gcgcagtttt acgtgcggca gaactgcgcc
    18421 acaggaaccc acgcggataa ttttcttcac gccgaaatcg gtgatcagtt ctttggtgta
    18481 gatggagcag gacgggatac ccataccgtg acccattacg gaaattttgc ggcctttgta
    18541 agtaccggtg aagcccagca taccgcgaac gttgttcact tcacgggcat cttcaaggaa
    18601 agtttcagca atatacttcg cacgcagcgg gtcgcctggc atcaaaacta cgtcagcgaa
    18661 atcgcccatt tctgcattaa tgtgtggggt agccattgtt ttatccttat caacatgttt
    18721 tcaccctctc cctgagggag agggccgggg tgagggcatc aagccgcacc cattatcaga
    18781 acatggcttt gccatattcc atatcagaag taccaaaata ttttgccaga gtctggccga
    18841 tatccgcgaa ggtttcacgg tgacccagtg agcccggttt tactttcggg ccgtaaacca
    18901 gtaccggaat gtgttcacgc gtgtggtcag taccggtcca ggtcggatca caaccgtggt
    18961 cagcagtgag gatcaggatg tcgtcatcgc gcagcagaga catcagctcc ggcagacggc
    19021 ggtcgaacag ctccagaccc acagcataac cggcgacgtc gcgacggtgg ccccaggaag
    19081 agtcgaagtc aacgaagttg gtgaagacga tggtgttatc acccgcttct ttcatctctt
    19141 tgatggtggc gtcaaacagc gcgtccaggc cagttgcttt cactttcttg gtgataccgc
    19201 agttggcgta gatgtctgca attttaccga cagaaaccac ctgaccgtgt ttttcatcaa
    19261 ccagtttctg cagcacggtc ggtgctggcg gctcaacagc caggtcgtga cggttaccgg
    19321 tacgctggaa gttaccggct ttgtcaccga taaacggacg agcgataaca cgaccgatgt
    19381 tgtagccgcc gttggtcagc tcttcacggg cgatttcgca cagttcgtag agtttatcca
    19441 ggccaaaggt ttcttcgtgg caggcaatct ggaacacaga gtcagcggag gtatagaaaa
    19501 tcggcttgcc ggttttcatg tgctcttcgc ccagctgatc cagaatgacc gtaccggaag
    19561 agtggcagtt accgaggtaa cccggcagat tagcgcgttc aaccagttta tccagcagct
    19621 cttgcgggaa gctgttttcg tgatcggaga aatatcccca ctcaaataga accgggacac
    19681 cggctatttc ccagtgacca gacggggtat ctttaccgga tgacatttcg tgcgcccatg
    19741 cgtacgcgcc gataacttca gcgttgccgt ccattcccgc cggaatgaaa ccggtagaac
    19801 cttcgtgtgc tttcgccagc cccagacggg tcagatttgg cagattgagc ggacctttac
    19861 gaccgttatc agcttcgcct ttggcacaag cttctgcgat atgacccagg gtgtcagccc
    19921 cgacgtcacc aaagcgttct gcatcttctg tagcgccgat gccgaatgag tccagcacca
    19981 taataaatgc acgtttcata tgttctccgt actcagtgct tcaaatttaa atgatcagat
    20041 cagtataccg ttattcgctg atacggcgat agacagttgg tgtgctttcc ggtgctttat
    20101 cggcaagttt aattgccgct ttcaccgctt tcgccgcttc ctgccagctg ttttcgtctt
    20161 tcgcgtggat aaccgccagc ggacgctgac cgtctacctg gtcgcccaaa cgcgccatat
    20221 cagtaaagcc gacgctgtaa tcaatggtgt cagatgcctg acgacgaccg ccgcccattg
    20281 caaccactgc catccccagc gcgcgggtat ccatttcact gacaaaacct tcggtatcag
    20341 catagactgc tttcgtcagc atcgctgtcg gcagatactt cgcgtagttc tcaacgaagt
    20401 cggttgggcc tttttgtgcc gctaccatac gaccaaagac ttctgccgct ttaccgttgt
    20461 ccagcactgc ttgcaatttc gcgcgcgctt cggcgtcatc tttcgccagt ttgccggaga
    20521 taagcatctc cacgcacagc gccatcgtga catcaaacag acgcgggtta cgatactcac
    20581 ccgtcaggaa ctgcaccgct tcacgaactt caaccgcgtt acctgcactg gaggccagta
    20641 cctgattcat gtcggtgagc agcgcggtgg tgcgcacgcc agcgccgtta gccacgccaa
    20701 caatcgcttc ggcaagggct tcagagagtt cgtaggtcgg cataaacgcg ccgctaccca
    20761 ctttcacgtc catcaccagc gcgtccagac cttccgcaag tttcttcgcc agaatagagg
    20821 cggtgatcag cgggatggag tccacggttg cggtaatatc acgggtcgcg tagaaacgtt
    20881 tatccgccgg agccagtgag ctggtctgac cgataatcgc cacgccgacg tctttaataa
    20941 tttcgcggaa acggttgtca tccgggaaaa tgtcgaagcc agggatggat tccagtttgt
    21001 cgagcgtacc gccagtatga ccgaggccgc gaccagagat catcggaata tagccgccgc
    21061 aggctgcgac catcggcccc aacatcagcg aagtcacatc gccgacgcca ccagtggaat
    21121 gtttatcaac aatcgggcca ttcagatgca ggcttttcca gtcgagaacg gttcctgaat
    21181 ctcgcatcgc catggtcagc gagacacgct caggcattgt catatcgtgg aagaaaatgg
    21241 tcatcgcgag ggcggcaatc tgcccttcgg agatagtgtt gtcgcgaatg ccgttgataa
    21301 agaaacgaat ttcttcatcg ctcagtgcat gaccatcacg ttttttacga ataatttctt
    21361 gtgcgagaaa caaggtaacc tcccgaggaa aagagtatga ggcggctttt gccggatgcg
    21421 tctacgcctt atccggccta cgggtagata cgtctcgtag gcctgataag cgcagcgcat
    21481 caggcgtaaa gcatcttact tagtagctgc tggcgctctt accgtcaccg tggcccagcg
    21541 ctttcaacag gcttgccagc aggctggaag caccaaagcg gtagtgacgc gcatctgccc
    21601 agtcagcacc gaacagttca tctgcgatgg cgagatattt ctgcgcatct tccgcagtac
    21661 gcacgccgcc cgctggtttg aaaccaacgg ttttttctac gcccatatca cggatcactt
    21721 ccatcatgat gcgcgcgctt tccggcgtcg cgttcacagc cactttaccg gtagaggttt
    21781 tgatgaagtc cgcacccgct ttgatggaga tttcagacgc tttacggatc agcgcttcgt
    21841 ctttcagttc gccggtttcg atgatcactt tcagcagtac attcgctgcc gcgcaagcct
    21901 ctttacaggc tttcaccagg tcaaaaccaa cctgctcgtt acccgccatc agcgcgcggt
    21961 acgggaacac aacgtcaact tcatcagcac cgtaggcgat tgccgcacgg gtttctgcca
    22021 gcgcgatttc gatgtcatcg ttaccgtgtg ggaagttggt taccgtagcg attcggattt
    22081 ccggggtgcc ctgctctttc agcgttttgc gagcaatcgg gataaagcga ggatagatac
    22141 agatagcggc ggtattgccg accggggttt tggcctgatg acacagagca attactttct
    22201 cgtcggtgtc gtcgtcattc agggtggtca ggtccatcaa tttcagtgca cgcaggctgc
    22261 ttgctttcag atcagtcatt tcattctcct ggcttgtcgc caataaaatt caccttgcga
    22321 gtttgttagt attctaacat ctactccgca acacacttcg atatacatca caattaagga
    22381 aatctactta caagtttgca tcactgtaat gcgatctggt tcaaataatt cactttcaaa
    22441 tgaatgcgtc agtggtggca aacgcatcag gatcaaagtg aacatcacga aacttcttac
    22501 aatggcgcat caccttcggc ataaaaggaa aagaagatgc ccacttctca tgaaaatgca
    22561 ctgcaacaac gttgccagca aattgtcacc agcccagtgc ttagcccgga gcagaagcgc
    22621 cattttctgg cactggaagc agaaaacaat ctgccttatc cgcagcttcc tgccgaagcc
    22681 cgccgcgcgc tggatgaggg tgtaatctgc gatatgtttg aaggtcatgc gccgtacaaa
    22741 ccgcgctatg tcttacccga ttacgcccgt tttctggcga acggttccga atggctggag
    22801 ctggaaggcg cgaaagatct tgatgacgca ctctctctgc tgaccattct ttaccaccac
    22861 gtaccgtcgg tcacatcgat gccggtctac ctggggcaac tggatgcgtt gttgcaacca
    22921 tatgttagaa ttctaacaca agacgagatc gatgttcgaa taaaacgttt ctggcgttac
    22981 ctcgacagaa ccctgccaga cgcctttatg cacgccaata tcggcccgtc tgattcgccc
    23041 attacccgcg cgatcttacg tgcagacgca gagttgaagc aggtttcacc gaacctgacc
    23101 tttatctacg atcctgaaat cacccctgat gacctgctgc tggaagtggc gaagaacatc
    23161 tgtgaatgta gcaaaccgca catcgccaac ggtccggtgc atgataaaat tttcacaaaa
    23221 gggggctacg ggattgtgag ctgttacaac tcactgccgc tggcgggtgg tggcagcacg
    23281 ctggtacgcc ttaacctgaa agccattgcc gagcgcagcg aatcgctgga tgacttcttt
    23341 acgcgcactc taccgcacta ctgccagcag cagatcgcca tcatcgatgc gcggtgtgaa
    23401 ttcctctatc aacaatcaca cttctttgag aatagcttcc tggtgaaaga agggctgatt
    23461 aaccctgaac gttttgtgcc aatgtttggc atgtatgggc tggcggaagc ggttaacttg
    23521 ctgtgtaaga aagaagggat tgccgcgcgc tacggtaaag aagccgccgc aaatgaagtg
    23581 ggttatcgca tcagcgcgca actggcggag tttgtcgcca atacccccgt gaaatatggc
    23641 tggcaaaaac gcgccatgtt acacgcacag tcggggagca gttccgatat cggcaccacg
    23701 ccgggcgcgc gtttgccgta tggtgatgag ccagatccga tcacccatct gcaaactgtc
    23761 gccccgcatc atgcttatta ttattccggc atcagcgaca ttctgacgct cgacgaaacc
    23821 atcaaacgta atccgcaggc gctggtacag ctttgcctcg gtgcctttaa agccggaatg
    23881 cgtgaattta ctgccaatgt cagcggtaac gatctggttc gcgttaccgg ttatatggtg
    23941 cgtttgtcgg atttagaaaa atatcgcgcc gaaggttcac gcaccaacac cacctggctg
    24001 ggcgaagaag ccgcacgcaa cactcgtatt ctggaacgcc agccgcgcgt gataagccat
    24061 gaacagcaga tgcgctttag tcagtaagat tatccccttc tcctgcgttg acgggccagg
    24121 cagtcgtctg gctctgtttt tgcagggctg caatctgcgc tgcaaaaact gtcacaatcc
    24181 gtggacgatg ggacgttgca atgactgtgg ggagtgcgtg ccacagtgtc cgcatcaggc
    24241 gttgcagatt gttgacggca aagtggtgtg gaacgctgtg gtttgcgagc agtgtgatac
    24301 ctgcctgaag aggtgtccgc aacatgccac gccaatggcg caatccatga gcgtggacga
    24361 agtgcttagc catgtccgca aagcggtgct gtttattgaa gggataacgg taagcggcgg
    24421 cgaagccacg acccaactgc cgtttgtagt ggcgctgttt actgctatca aaaacgatcc
    24481 gcaactgcgc cacctcacct gcctggtgga cagtaacggc atgttgagcg aaaccggctg
    24541 ggaaaaatta ctcccggtgt gtgacggcgc aatgctcgat ctcaaagcgt gggggagcga
    24601 atgtcatcaa cacttaaccg gacgcgataa tcagcagatt aagcgcagca tctgtttgct
    24661 tgcagagcgc ggcaagctgg cggaactgcg tttgctggta attccagacc aggtggatta
    24721 tttgcatcac atcgatgagc tggcgacgtt tatcaagaga cttggcgatg ttccggttcg
    24781 cctgaatgcg tttcatgccc acggcgtgta tggcgaggcg caaagctggg cgagcgccac
    24841 gccggaagac gttgagccgt tggctgatgc gttaaaggtg cgcggggtga gccggttgat
    24901 atttccggcg ctctatttgt gacatgccgg atacggccta cggttcggca cagacttgta
    24961 ggcatgataa gacgcgacaa gcgtcgcatc aggcattatg cgccgactgc cggatgcggc
    25021 gtgaacgcct tatccggcct acggttcggc acagacttgt aggcatgata agacgcgtca
    25081 gcgtcgcatc aggcattgtg cgccaactgc cggatgcggc gtgaacgcct tatccggcct
    25141 acggttcggc acagacttgt aggcatgata agacgcgtca agcgtcgcat caggcattgt
    25201 gcgccaactg ccggatgcgg cgtgaacgcc ttatccggcc tacggttcgg cacaaaccag
    25261 taggcatgat aagacgcgtc aagcgtcgca tcaggcattg tgcgccaact gccggatgcg
    25321 gcgtgaacgc cttatccggc ctacggttcg gcacaaacct gtaggcatga taagacgcgt
    25381 taagcgtcgc atcaggcatt atgcgccgac tgccggatgc tgcctacgaa gggtaggcta
    25441 cccactaacg ctaaacaacg catacgtatt attaagcaac acttcggcaa tctcatcttc
    25501 tggttccggg cgcaactcgc aaagcacgtc aaacacccgc acagcctgtt ccggtcggtt
    25561 aggctgcccc tgaaagccgt tgagcggcat atccggcgcg tcggtttcca gcaacaacga
    25621 tgccagcggt aattttgcga tgacatcgcg ggttttactg gcgcgtggat aggtgatagt
    25681 accgcctacg ccgattttgt agcccagctg tacaaaccgc tcggcctgtt gcaggctgcc
    25741 ggaaaaaccg tgaaccacgc cagtgcacgg taaatcgtga cgtttaagat gcatcgccag
    25801 tttgtcgtgt atacgccggg aatgaaggat caccggcaga tcgtagcgtt tcgccagttt
    25861 cagctgttcg tcgagtaacc actgctgcct ctcaaattgc ggatcgtcgc cgaagagatc
    25921 cagaccgatc tcccccaccg ccaccacctt cgccggacgc ctttccagcg cctgctgtag
    25981 ctgatcaaga gacacatcgc tatgtttttc caacatcccc ggatgcaacc ccaatgcggc
    26041 atacagcggt tgatattttt ccgctaacgc ctgcacgcgg gcaaaatttt ccgcctctgt
    26101 cgccggaaca atgatcttgc ctacgcccgc ttgtgccgcg cgttgcaggc tggcctcttc
    26161 atcgccacta aacggcggga aatcaaaatg gcagtgggta tcgataaaac ggcaaatcaa
    26221 gccagatcct cattgttaaa tgtcgggtcg ttcgcctgcg gagcatcgct cacttcggca
    26281 accagcgtat cgttggcgac tggcgcaggc ggaataacga tcgattccgg cgtcaccact
    26341 ggcaccagat ggcgggtaag cggcgctttt tcagttaata gcttgccaac cgtggcgagg
    26401 aaataacgac cgcataaacg cccaagttta tagtcttcac gcagcgccgg aatccgactg
    26461 ccaagcgcga tactatgtaa tggcttcggc ggataaattt cgaatatccg cagcttgccc
    26521 ggtggtttct caataaattg ctgaatgtca cgatagctgg tttcatgatg ctgcaccaga
    26581 ttgaccagcg gctgcaggct actgtcaccc agccagcgtt ccatgcgttt gaaccactgc
    26641 ggcgtgtagt acatttgtga cggcacagtg cgaatgacga ccaacgtttt agcgccctgc
    26701 cttgccgcct ctttaaccgg aatcgcatca ctgatcccgc catccaggta gttaatgcct
    26761 tccagcgaca ctccgctacg ataaaagcca ggtatcgcac tggaggcgcg aatcacatcc
    26821 agccagtttt gtttggttgg taaaaagtaa ttaggcgcgt agtcatcctg acgacaggcg
    26881 cacatataaa acgatttgcc gctgtcaaac aaccgcgcgg cggtatccat ttgcaacggc
    26941 atctggcttg cggtggcctc caccagccag tcgagatcga taagatttcc tccacggaca
    27001 aagcgcaatg gatcgaaaaa ttcgcgtttt gtggtatagc gcatgatgac tttgcgcgcg
    27061 taaccgggct gattgcagat aaacgccgag aggttctgcg ccccggcaga tgtgccgaga
    27121 taaagatcga aaggattaaa ctgcgcgcgc ataaactcat ccagcacgcc agccgtgaaa
    27181 attccacgct gtccgccgcc ttcgcacacc agagctattc gtccaggctg gaacggcctt
    27241 agcgacaacg gcgcaatatt accaagcgtt acaggtattc gctgccccac ctctgccttc
    27301 ctgtttttgt tgtgaaagaa tgacaaagta acgcaattta ttattcgcta aaaccgcaaa
    27361 agccagtccg ctggactggc tttggataaa attttcttaa tggtctgaaa tctagggtcg
    27421 ttttcggccc atgaacaaac tcaccaggaa cagaataatc ccgacgacaa agacaatttt
    27481 agctgcgcct gcagcggtac cggccagacc accaaaccca agtgcggcgg cgattaacgc
    27541 gataaccaga aatatgatgc cccaacgaaa cataagcgtc tcctttacca tagttaatgt
    27601 caccgcttga tatgagcgtg aaaatcactc aatgcgatat ttttagtgtg gtgcacatcg
    27661 cgcctcccga caaagttcgg gaggacgaat tacgacgaat tacttagttt tcagatcatt
    27721 tttaacgctt ttcacaccat ctaccgcttt ggcgatactt tcagcacggt cgctctgtgc
    27781 ctgggaatcg acggtaccgg agagctgaac cacgccgtcg gtggtttcaa ctttcacatg
    27841 acgggaaggg acgatatcgt ccgccagcag tttggctttg atttcactgg tggtggcggt
    27901 gtcacccgcg tagcccttca cagagccttc tttagcgtcg cgaacgtgca gtttgtcgct
    27961 gacagaggtc accccttcaa cgcctttcgc cactttcact gcctcttcgg cctgagcctg
    28021 gctttcaacg aaaccgctca gggtcacgac tttttgatcg gttttgacag agatatcggt
    28081 gctcttgatg ttgtcatgat ccaccagcgc cgccttcact ttcgcggtga tggcgctgtc
    28141 atccatgaaa ttaccgactt tattcataga gctatcgact ttttgccctg cgctttcatt
    28201 ggtagtctgt gcgttgtttt ccgcgtaggc agagccggtc gcgacggcag aggtcaacat
    28261 tacagccagc agagttttcg aaatcttcag tcttgtcata gtcatcgatt tattcctgta
    28321 tgtttgctcg taatttgagc ctggcaacac gaggttgcat tgctgaatag ggagagactt
    28381 caccctctac agaagtcaat ggtcgccatc acaaaagcga tgggtgatga ataacgacca
    28441 ttacagcctc tgaatcagtt attaatatcg gtagaatgac aatcgacggc tttagatact
    28501 gatatctacg cattgaacgg tatttaacgc cgtcagaaat gtcattactt tgttaaatat
    28561 agatcacaat tttgaaaccg ctcgggatat cagcgaaaac ataagcaaaa gtgaatgttt
    28621 taagaacatt ccgtaagcgg ctaataagga agggaaattg acagggcgca gcggctaccg
    28681 cgccctggag gcaagaatta atgctcgcgg gtctggtgga actgaacgtc gggataacgt
    28741 tcctgcgcca ggcgcaggtt gaccatgctg gtagcgatgt aagcgaggtt atcgccgcca
    28801 tcaagcgcca gttggctttc gttcttacgc ttgaactctt cgaatttctt cgcgtccgca
    28861 cattctaccc agcgggcagt ggcaacgttg actgattcat atactgcttc aacgctgtat
    28921 tcgctcttca ggcgcgatac caccacatca aactgcagca caccaacagc accaacgatc
    28981 aaatcgttgt tggagatcgg acggaacacc tgcaccgcgc cctcttcgga aagctgtacc
    29041 agccctttga gcagctgttt ttgcttcagc ggatctttca ggcggatacg acggaacagt
    29101 tctggtgcga agttcggaat accggtgaac ttcatcatct caccctgggt aaaggtgtcg
    29161 ccgatctgaa tagtgccgtg gttgtgcagg ccgaggatat cgcccggata cgcttcttca
    29221 acgtgcgaac ggtcgcccgc cataaaggtc agcgcgtcgg agataaccac atctttcgca
    29281 gtacgcacct ggcgcagctt catgcctttt tcatatttac cggatactac gcgcataaac
    29341 gccacgcggt cgcggtgttt cgggtccatg ttggcctgaa ttttaaatac gaagccggta
    29401 aatttgtctt cgctcgcctc tacggtacgg gtatcagtct gacgcggcat cggcgcagga
    29461 gcccactcca ccaggccatc cagcatatga tcaacgccga agttacccag cgcagtaccg
    29521 aagaataccg gagtgatttc gcccgcaagg aacaattctt tgtcgaactc gttagacgcg
    29581 cctttcacca gttccagttc gtcacgtagc tgctgtgcca gatcttcacc aaccgcagcg
    29641 tcgagatccg ggttattcag ccctttaaca atacggactt cctgaatcgt gtggccttta
    29701 ccgctctgat agagataggt ttcgtcttta taaaggtggt aaacgccttt aaacagcttg
    29761 ccgcagccaa tcggccaggt gatcggtgcg cagccgattt tcagctcgtt ctcaacttca
    29821 tcgagcagtt ccatcgggtc gcggatatca cggtcaagtt tgttcataaa ggtgaggatc
    29881 ggcgtgtcgc gcagacgggt aacttccatc agcttacggg tacgatcttc aacacctttt
    29941 gcagcgtcga taaccatcag gcagcagtcc accgccgtca gggtacgata ggtatcttcc
    30001 gagaagtctt cgtgccccgg ggtgtcgagc aggttaacca ggcaatcgtg atacggaaac
    30061 tgcatcacag acgtagtaat ggagatccca cgctgctttt ccatctccat ccagtccgac
    30121 ttagcgtgct ggttggaacc acggcctttt actgtaccgg cggtctgaat ggcctgtccg
    30181 aacagcagca ccttctcggt gatggtagtc ttaccggcat ccgggtgaga aataatggca
    30241 aaagtgcggc gcttcgccac ctcttgcaaa taaggagaca acgtcataat tctttcttct
    30301 tgagtaagcg gcatcgcgcc gcgcttgttg aaaacgaaaa attgcggcta ttttacccat
    30361 caacgggggg gaggcaatca gtgtttacac aggagctgct ccagttcgtg caacgaagaa
    30421 acggtccagg tgggcgcgat gccttctggt tgctcgcgat tgtgcgcatt cagccagcag
    30481 gtcgcaagcc cggcgttgat gccaccgaga atatcggact cggcagtgtc gccaaccatc
    30541 agcacgcgtg aacggtcagg attgcccgcc tgttccagcg cataatcgaa aattttctta
    30601 ttcggtttgg caacgccaac ttcttcggaa atcaccagca aatcgaagta atcacgcagg
    30661 cccgtgcgtt ccagacgcac ttgctgcaag gcgctaaagc cgttggtgat gatgccgatt
    30721 ttggcgttgc cacgaatggc gttaagcaga gaaaccgcgc ccggcagcgg cgtgcagatt
    30781 tccgccatcg cattaataaa ggcatcgtta agcaagcctg cttcaacttt cagccgttcg
    30841 gcccagctct caaaacgccc gtgctgaagc tgtaatgaag tgatcgcgcc gttttgataa
    30901 tccacccaca gtggcttgtt aacggcctga tagtcctgaa aatcttcagc ggtaaaggtg
    30961 acgctgtaat caagaaacat ccgctgcagg ccggtgaatg agtcaaaggt aaacagcgtt
    31021 tcatcggcat caaagaaaat ccagtcccac ttcattattc caccttgtct tacatactga
    31081 ttggcaacgc catgatgatg gcgtcttcgc gaccgtccgt ggtggggtag taattgcggc
    31141 gaatcgtcgc ctcgttaaag cctaaacttt cgtacagggc aatggcggca gcatttgagg
    31201 cacggacttc cagccatagt gtcgccacgc cgcgtttttc cagttcgtcg atcagatgtt
    31261 ccagcagcac ccttcccaat ccctgacgct gatagtcagg atcgaccgca atattgaaca
    31321 atgtagcttc atccagcacc acttgcgtaa tcgcaaacgc cgccattttg ccgttttgcg
    31381 ttaactgaaa gttgagataa cgctcgccct ggttgctggc aaaggttttt tcactccacg
    31441 gaaaggcgtg ggcgcgttgt tcaatgtggt aagccgctgg taaatcagtc gtttcgaggg
    31501 aagaaatcgt gttcatatgt gcaaatctgt tgccataacg cggcgcgtgc cgttgggttt
    31561 gcccgtaatt cggtgagcgc cggtgatgcc acctgagcgc cttccagtga tagcggttcg
    31621 tcagtaccca accgccagct gttgcagcga ctgccttgcg gcagcatcgc gattttttct
    31681 ggcgtcagtt gcagcacttg atcggggctg acggttaatg cgcgcagaac atcgctcact
    31741 aaaggatcgg tcagggcggg aagatcgttt gccaccatca ccagacggac gtgtgccggg
    31801 atcgcaatgg caatctcccc ctgcaacgcg ccagggcgac gcagcgacca ctgggtaatg
    31861 cccagttgct gtaactgcca gtctcgtcgg gatgtcatag cgaatcgctc ctgttgtcag
    31921 gggcgcaaat atagcaaatt cgtcgatacc gcgccaacat atggctataa tcgccgccag
    31981 tatcaattga ggagcattcc atgtctgcat ttaccccggc aagtgaagtc ttgctgcgtc
    32041 acagtgatga tttcgaacaa agccgtattc tgtttgccgg agacttacag gatgacctgc
    32101 ccgcgcgttt agataccgcg gccagccgtg ctcataccca gcaattccac cactggcagg
    32161 tattaagccg ccagatgggg gataacgccc gtttcagtct ggtcgccacg gtggatgacg
    32221 tcgcagattg cgatatgctg atttactact ggccgaagaa caaaccggaa gcccagttcc
    32281 agttgatgaa tttactttct ctgctgccag tggggacaga tatttttgtc gttggcgaga
    32341 accgcagcgg cgtgcgcagc gccgagcaga tgctggcaga ttatgcgccg ttgaataaag
    32401 tcgacagcgc tcgtcgctgt ggcctctatt ttggtcgtct ggaaaaacag ccggtatttg
    32461 atgccgataa attctggggc gaatacagcg tcgatggcct gacggtcaaa acgctgcctg
    32521 gcgtgtttag ccgcgacggt ctggatgtcg gtagccagtt gctgctctcg acgttaactc
    32581 cgcacacgaa aggtaaagtg ctggatgtcg gctgtggcgc gggggtgctt tcagttgcct
    32641 ttgcgcgcca ttcgccgaaa attcgtctca ccttgtgcga tgtctctgcg ccagcggtag
    32701 aagccagccg cgcaacactt gcggccaact gtgttgaagg tgaagtcttt gccagcaacg
    32761 tcttttccga ggtgaaaggt cgttttgata tgatcatctc caacccgccg ttccacgatg
    32821 ggatgcaaac cagcctggat gcggcgcaaa cgctgattcg cggcgcggtg cgtcatctta
    32881 atagcggcgg cgagctgcga attgtagcga acgccttcct gccttacccg gacgtgctgg
    32941 atgagacatt tggcttccat gaagtgattg cgcaaaccgg gcgtttcaag gtgtatcgcg
    33001 ccattatgac ccgccaggcg aagaaaggtt aattatctcg ccgaataccg tgtcggatgc
    33061 ggcgtgaacg ccttatccga cctacaaaac ctgcacgtta gcccttcgta ggccagataa
    33121 gacgcgccag cgtcgcatct ggcatctgcc cccgcatgcc taccaaacct gcacgctagt
    33181 ttcctgatgg acatttttcc agcaattaca cctccggcga taattaacta ttgacgaaaa
    33241 gctgaaaacc actagaatgc gcctccgtgg tagcaattct ttttaagaat tgatgg
//
LOCUS       DAZPAC010000020        32837 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZPAC010000020 DAZPAC010000000
VERSION     DAZPAC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.020"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            267..1925
                     /locus_tag="ACHUPM_000803"
     CDS             267..1925
                     /locus_tag="ACHUPM_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR0868669.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="ACHUPM_000804"
     CDS             complement(1922..2878)
                     /locus_tag="ACHUPM_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR0868670.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="ACHUPM_000805"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="ACHUPM_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR0868671.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="ACHUPM_000806"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="ACHUPM_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR0868672.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHUPM_000807"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHUPM_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR0868673.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6534..6608
                     /gene="yljB"
                     /locus_tag="ACHUPM_000808"
     CDS             6534..6608
                     /gene="yljB"
                     /locus_tag="ACHUPM_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR0868674.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="ACHUPM_000809"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="ACHUPM_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR0868675.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="ACHUPM_000810"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="ACHUPM_000810"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR0868676.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="ACHUPM_000811"
     tRNA            complement(9740..9827)
                     /locus_tag="ACHUPM_000811"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHUPM_000812"
     CDS             complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHUPM_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR0868677.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHUPM_000813"
     CDS             complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHUPM_000813"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR0868678.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHUPM_000814"
     CDS             complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHUPM_000814"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR0868679.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHUPM_000815"
     CDS             complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHUPM_000815"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR0868680.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHUPM_000816"
     CDS             complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHUPM_000816"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR0868681.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16451..16945
                     /gene="lrp"
                     /locus_tag="ACHUPM_000817"
     CDS             16451..16945
                     /gene="lrp"
                     /locus_tag="ACHUPM_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR0868682.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHUPM_000818"
     CDS             17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHUPM_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR0868683.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21302..21913
                     /gene="lolA"
                     /locus_tag="ACHUPM_000819"
     CDS             21302..21913
                     /gene="lolA"
                     /locus_tag="ACHUPM_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR0868684.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21924..23267
                     /gene="rarA"
                     /locus_tag="ACHUPM_000820"
                     /gene_synonym="mgsA"
     CDS             21924..23267
                     /gene="rarA"
                     /locus_tag="ACHUPM_000820"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR0868685.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23358..24650
                     /gene="serS"
                     /locus_tag="ACHUPM_000821"
     CDS             23358..24650
                     /gene="serS"
                     /locus_tag="ACHUPM_000821"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR0868686.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHUPM_000822"
     CDS             24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHUPM_000822"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFR0868687.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHUPM_000823"
     CDS             27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHUPM_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR0868688.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHUPM_000824"
     CDS             27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHUPM_000824"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFR0868689.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHUPM_000825"
     CDS             complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHUPM_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR0868690.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29803..30951
                     /locus_tag="ACHUPM_000826"
     CDS             29803..30951
                     /locus_tag="ACHUPM_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0868691.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31161..32591
                     /locus_tag="ACHUPM_000827"
                     /pseudo
     CDS             31161..32591
                     /locus_tag="ACHUPM_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
     gene            complement(32592..>32837)
                     /locus_tag="ACHUPM_000828"
     CDS             complement(32592..>32837)
                     /locus_tag="ACHUPM_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0868692.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         8057 a         8030 c         8813 g         7937 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct
     9841 accaaacctc atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag
     9901 aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc agtatatcta
     9961 catcgagaca agttacggac ttatacttcc aaagtacttc atacatatca caaaataaaa
    10021 aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa
    10081 tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt
    10141 cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc
    10201 agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag
    10261 aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact
    10321 accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg
    10381 ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca
    10441 aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat
    10501 tccttatcgg cagcggggta atgagcataa ccaactctgc gaccgcaatt ataacactct
    10561 ggggagaaat gtgccgaaaa cattcatttt tgtggtgaaa acaagcatcg tggcacccag
    10621 aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattcagata atcccgacgg
    10681 ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca gtcgataagc
    10741 ttaccgccat gactgataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt
    10801 tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac gccgtacata
    10861 ccgccgacaa gctcatcttc atgccagact tcaatggagt gggcatgacc gagttcgtga
    10921 aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg
    10981 gcacaacctt caatgacctg accaaaagcg taattcatcg tgacacgata gggcgagcgt
    11041 ttatgaaatc gcttcatact acggctgata tgcagtgatt caggccaaag caccgcgcga
    11101 ggatcgggcg accaccagag gatgggggcg cctggagaaa accacggaaa aataccacgc
    11161 tggtaagcca ttaacaggcg cgcagggcta agatcgcccc ccagagccag caggccgtta
    11221 ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc
    11281 aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct
    11341 gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa
    11401 taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat
    11461 gggtgaccat taacaccgtt ttctcacgca tcatttctgc aagcaattca aggatctggc
    11521 tttcggttgt ggcatctaag ccttcggtag gttcatccag caacaccagt ggcgcatcat
    11581 gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc tggcgtccgc
    11641 cttcacctaa ccaactgttg agacctgcgt cctcgagcag cttctccaga ccaacgcgac
    11701 gcaagatctc cgacagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac
    11761 gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag
    11821 ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc tgttgcgggt
    11881 cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa
    11941 tcgcaatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt
    12001 gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg gcaacacgag
    12061 tttgggtatc agggaaggtg acttccggtt tttgatccgt taaatcagtg atccgaacgg
    12121 cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa
    12181 acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc
    12241 caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca
    12301 ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc agccattgaa
    12361 tttctgtatt ctctagttgc gtgcgataac gatcgctggc accaaaaatg gtcagctcag
    12421 cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga tgagtcagat
    12481 tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa agcgtcagta
    12541 acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgactgtca
    12601 ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat
    12661 cgagcgtatc aacatccgcc accacgcgat tgagcaattc gccctgacga tagcgcgcca
    12721 gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga tgctgcaaca
    12781 cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca gtacgggtga
    12841 ttgcggcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcac
    12901 cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacaga ccgatactgg
    12961 cgagcagcgt cacaattgcc agcacaatac caagacttaa catccattta tgacgtttat
    13021 acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagta
    13081 atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc tcaataatct
    13141 gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga
    13201 ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg cgctgttcac
    13261 tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta
    13321 acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca gcctggtcgc
    13381 caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc caggcgttat
    13441 ccagcgctgc ttgtaattca tgttcgctgg catcaggtcg cgccagtagt acgttatccc
    13501 gtaatgttgc tgccggtaat tgtggatttt gcccaaccca ggagagatgt ttacgccatg
    13561 attctggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc tgatatgaga
    13621 gaaaaccaga aagcgcgttc aacagtgaac ttttacctga accgctgcga ccaaccaaca
    13681 cggcacgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt ttaccttccg
    13741 gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg atcggtcgat gctaattcca
    13801 cctcgccacg ctgcggatgg gcgagcgggg tttccataaa cgttttcaga ctgtcagctg
    13861 caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa
    13921 actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcgtagt
    13981 gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg
    14041 acagcgaggt aaaaaattcg agaatgccgg aggataaaaa tgccagccgt agcacttcca
    14101 ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac
    14161 gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc
    14221 gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca
    14281 taaacaacgg aattaacggt gcagtgccca gcagaatgag cgccgcagcc cagtttgagg
    14341 ggaagattgc caccacaatc agcaacggca ccgacactgc cagcgccatt tgcggcaggt
    14401 agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc
    14461 ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac
    14521 ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga
    14581 ccgcccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt
    14641 cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg
    14701 caatgatcaa tatgccgctt acaaagccca gcagacgaga aatattcagc caacgttggg
    14761 agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgagat ttattcattg
    14821 cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac ggggaaaaaa
    14881 taaaggcgac ccatagtcgc aaggtgtcgc cttctttact tttgttactg atttgtaaaa
    14941 ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc
    15001 agtaccggcc gaagtaatgg cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa
    15061 gacgccagga atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta
    15121 gccgttttcc agttccagct gcccttcgaa aatcgcagta ttcgggctgt gaccgatagc
    15181 aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag
    15241 acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtac ggttggtgtg
    15301 cagaatgatg ttgccgttct ccactttatc catcagacgc ttaataagga ttttttccgc
    15361 gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag acagatacag
    15421 cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa
    15481 gaaaccgtcg caggttgcac aagcagaaac cccacggcct ttaaaggctt cttcagaggg
    15541 caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc
    15601 gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt tgatatgatc
    15661 aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc atgcgctcca ttaataacgg
    15721 accggtcaga tcgtttggat caccaggcca gttttccact tccgtggtgg tggtcagttg
    15781 gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac
    15841 agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt
    15901 gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga
    15961 cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc
    16021 aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga
    16081 ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat
    16141 gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaaacagt
    16201 attcttatat gcgcataacc atgcatgtaa ataccatgtt taccgtgcta gtgaaatcta
    16261 cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc aacggtcttc
    16321 tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg
    16381 gtagcgactc tgaacagtga tgtttcaggg gcagacagga gtagggaagg aatacagaga
    16441 gacaataata atggtagata gcaagaagcg ccttggcaaa gatctcgacc gtatcgatcg
    16501 taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg
    16561 tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt
    16621 tattcagggc tatacggcgc tgcttaaccc ccattatctg gatgcatcac ttctggtatt
    16681 cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca ataccgctgt
    16741 acaaaaactt gaagaaattc aggagtgtca tttagtatct ggtgatttcg actacctgtt
    16801 gaaaacacgc gtgccggata tgtcagctta ccgtaagttg ctgggggaaa ccctgctgcg
    16861 tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca agcagagtaa
    16921 tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt attttgatta
    16981 cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt gtccgtttta
    17041 gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga atacactgaa
    17101 gacaaagaag tcacattgac aaagttaagt agcggccgcc gccttctgga ggcgttgctg
    17161 atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt taacccttcg
    17221 gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg tgggatgccc
    17281 ggtgcgtggt tggcagatac gctgttcttt atttttggcg tgatggctta caccattccc
    17341 gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagcga cgaatacatt
    17401 gattattttg ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct tacctcctgt
    17461 ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg cgtcattggc
    17521 agcttactaa gcactacgct acaaccactg ctacacagta gcgggggaac tattgcgctg
    17581 ctctgcgttt gggcagcggg cctgacgttg ttcaccggtt ggtcatgggt gaccattgct
    17641 gaaaaactcg gcggctggat tttaaacatt ctcaccttcg ccagtaatcg tacccgtcgc
    17701 gatgatacct gggtcgatga agatgagtat gaagacgacg aagagtatga agatgaaaat
    17761 cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt
    17821 aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagacgc tgcgttgttc
    17881 tccggtaagc ggatggatga tgacgaagag attacctaca ctgcacgcgg tgtggctgcc
    17941 gaccctgacg acgtcctatt ttcgggcaat cgtgcaacgc agccagaata tgacgaatac
    18001 gatccattat taaacggtgc gccaattacc gaacctgtcg ctgtagcagc tgctgctacc
    18061 acggcggcac aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc
    18121 tctgttgatg ttccaccttc gcaacctaca gtagcctggc agcctgtacc gggtccacaa
    18181 acgggagagc cggttattgc tcctgcaccg gaaggttacc cacagcagcc acaatatgcg
    18241 cagcctgcag tgcaatataa tgagccgctg caacaaccag tacagccaca gcagccgtat
    18301 tatgcacctg cagctgaaca acctgtgcaa caaccttact acgctcctgc ggcggaacaa
    18361 cctgttcagc aaccgtatta cgcccctgcg ccagaacaac cggtggcagg taacgcctgg
    18421 caagccgaag agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact
    18481 tatcagcaac cagccgctca ggagccgttg taccaacagc cgcaacccgt tgaacagcag
    18541 cctgttgtgg agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc gccactttac
    18601 tattttgaag aagtggaaga gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat
    18661 caaccgattc cagaaccggt taaagaacca gaaccgatca aatcttcgct gaaagcacct
    18721 tctgttgcag cagtacctcc agtagaaacc gctgccgctg tttctccgct ggcatctggc
    18781 gtgaaaaaag cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt
    18841 ctggcaaata gcggtggacc gcgtccgcag gtcaaagagg ggattggccc gcagttgcca
    18901 cgaccgaaac gtatccgcgt gccaactcgc cgtgaattgg cgtcttacgg tattaagctg
    18961 ccctcacagc gtgcggcgga agaaaaagcc cgtgaagccc agcgcaatca gtacgattct
    19021 ggcgatcagt acaacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag
    19081 ttcgcccaga cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgtaaac
    19141 gcagaagatg cagatgctgc ggcagaggct gaactggctc gtcagtttgc gcaaactcaa
    19201 caacaacgtt attccggcga acaaccggct ggggcgaatc cgttctcgct ggatgatttt
    19261 gaattttcgc caatgaaagc gttgctggat gatggtccac acgaaccgtt gtttacgcca
    19321 attgttgaac ctgtacagca gccgcaacaa ccggttgcac cgcagcagca gtatcagcag
    19381 ccacaacagc cggttgcgcc acagcagcaa tatcagcagc cgcaacagcc ggttgcgccg
    19441 cagccacaat atcagcagcc gcaacaaccg gttgcgccac agcagcaata tcagcagccg
    19501 caacaaccag ttgcgccgca gccgcagtat caacagccgc aacaaccggt tgcgccgcag
    19561 cagcagtatc agcagccaca acagccagtt gcgccacaac cgcaggatac cctgcttcat
    19621 ccgctgttga tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac gccgctgcct
    19681 tctctggatt tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt
    19741 gaacaaatgg ctcgcctggt ggaagcgcgt ctggctgatt tccgtattaa agccgatgtt
    19801 gtcaattact ctccggggcc ggttatcact cgctttgaat tgaacctggc accgggcgta
    19861 aaagcggcgc gcatttctaa cttgtcacgg gaccttgccc gttcactttc gacggtggcg
    19921 gtgcgtgtcg ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accgaataaa
    19981 aaacgacaaa ccgtttatct gcgcgaagtt ttggataacg ccaaattccg cgataatccg
    20041 tcgccattaa ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg
    20101 gcgaaaatgc cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg
    20161 aacgcgatga tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg
    20221 atcgacccga aaatgctgga gctttcggtt tatgaaggca ttccgcatct gttaacggaa
    20281 gtcgttactg atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag
    20341 cgtcgttata aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa
    20401 attgctgaag ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgac
    20461 agtatggatg cccagcatcc ggtgctgaaa aaagaaccat acattgtggt gttggttgac
    20521 gaatttgccg acctgatgat gacggtaggt aaaaaagtgg aagagctgat agcacgtctg
    20581 gcgcaaaaag cccgtgccgc gggtatccac ctcgtactgg caactcagcg tccatcggtt
    20641 gatgttatta ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc
    20701 agtaagattg actcacgtac cattcttgat caggctggcg cggaatcact gctggggatg
    20761 ggggatatgc tctactctgg gccgaactcc acgttgccgg tacgtgtcca tggtgctttt
    20821 gttcgcgatc aggaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag
    20881 tatgttgatg gcatcacttc cgacagtgaa agcgaaggtg gcgcgggcgg tttcgatggc
    20941 gctgaagaac tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa
    21001 gcgtcaattt ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt
    21061 atcgaacaga tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa
    21121 gtgctggctc caccgccgtt tgactaatta atgcattgcc ggataaggcg cggtagcgtc
    21181 gcatccggca ctctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt
    21241 agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt gaggaataat
    21301 gatgaaaaaa attgccatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg
    21361 ggctgatgcc gcaagcgatc tgaaaagccg cctggataaa gtcagcagct tccacgccag
    21421 cttcacacaa aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct
    21481 gtgggtgaaa cgtccaaact tattcaactg gcatatgaca caacctgatg aaagcattct
    21541 ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac
    21601 ttggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag
    21661 cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc
    21721 cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca
    21781 gtttagcgcg gtggagcagg acgatcagcg cagcagttat caactgaaat cccagcaaaa
    21841 tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga
    21901 tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt
    21961 ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggccagcaac
    22021 atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat ctgcattcta
    22081 tgatcctctg ggggccaccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct
    22141 atgcgaacgc tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg
    22201 aggcgatcga gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg
    22261 acgaagtgca ccgattcaac aaaagccagc aggatgcatt tctgccacat attgaagacg
    22321 gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac
    22381 tgctttcccg tgcccgtgtc tatctgttga aatccctggg tacagaggat attgagcaag
    22441 tactaactca ggcgatggaa gacaagaccc gtggttatgg tggtcaggat attgttctgc
    22501 cagatgaaac acgacgcgcc attgctgaac tggtgaatgg cgacgcgcgc cgggcgttaa
    22561 atacgctgga aatgatggcg gatatggccg aagtcaatga tagcggtaag cgggtcctga
    22621 agcctgaatt attgaccgaa atcgccggtg agcgaagcgc ccgctttgat aacaaaggcg
    22681 atcgctttta cgatctcatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg
    22741 cggcgctcta ctggtatgcg cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc
    22801 gtcgttgcct ggcaattgct tccgaagatg tcggcaatgc cgacccacgg gcgatgcagg
    22861 tggcaattgc ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca
    22921 ttgctcaggc gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt
    22981 ttaaagctgc gctggcggat gctcgcgaac gcccggatta tgacgtgccg gttcatttgc
    23041 gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc
    23101 atgatgaagc aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa
    23161 cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct
    23221 ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg
    23281 gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg
    23341 ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg
    23401 ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgaag
    23461 agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat
    23521 cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag
    23581 tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg
    23641 aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg ccggtaggta
    23701 aagacgaaaa tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag tttgactttg
    23761 aagttcgtga ccacgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagctgcag
    23821 ttaagctgac tggttcccgc tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg
    23881 cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg
    23941 ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg
    24001 gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga
    24061 tcccaacggc agaagttccg ctgactaacc tggtacgcgg tgaaatcatc gatgaagatg
    24121 atctgccaat taagatgacc gcccacaccc catgcttccg ttctgaagcc ggttcatatg
    24181 gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga
    24241 tcgtgcgccc ggaagactca atggcggcgc tggaagagat gactggtcat gcagaaaaag
    24301 tcctgcagtt gctgggcctg ccgtaccgta aaatcatcct ttgcactggc gacatgggct
    24361 ttggcgcttg caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgtg
    24421 agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc
    24481 gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtctggctg
    24541 ttggtcgtac gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag
    24601 taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt
    24661 ttctgaatct aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata ccgaacaata
    24721 attactcttc acttacacgt aatactactt tcgagtgaaa atctacctat ctctttgatt
    24781 ttcaaattat tcgatgtata caagcctata tagcgaactg ctatagaaat aattacacaa
    24841 tacggtttgt tactggaatc aatcgtgagc aagcttgagt gagccattat gaaaacgaaa
    24901 atccctgatg cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg
    24961 atcggcggct tggcaatggc cagcagcgca ttaacattac cttttagtcg gattgcgcac
    25021 gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag cgcctgtaca
    25081 gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga aatcaaatat
    25141 gtcgaaacgg acaataccgg cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgc
    25201 ctgcgtgggc gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa atatccgatg
    25261 aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga agaagcctac
    25321 gacatcatcg cgaccaatat gcagcgcctg atcaaagagt acggcaacga gtctatctat
    25381 ctgaactatg gcaccggtac gctgggcggc accatgaccc gctcctggcc gccgggaaat
    25441 accctggtcg cgcggctgat gaactgctgc ggcggctatc tgaaccatta cggcgactac
    25501 tcctccgcgc aaattgcgga aggtttgaac tatacctacg gcggctgggc agatggcaac
    25561 agcccgtcgg atatcgaaaa cagtaagctg gtagtgctgt ttggtaataa ccctggcgaa
    25621 acgcgaatga gtggcggtgg ggtgacttac tatcttgaac aggcacgcca gaaatctaat
    25681 gcccgcatga tcatcatcga tccgcgctat accgacaccg gtgccgggcg cgaagatgag
    25741 tggatcccta ttcgtccggg aacagatgcc gcactggtta acggtctggc gtacgtcatg
    25801 atcactgaaa acctggtgga tcaggcattc ctcgataaat attgcgttgg ctacgatgag
    25861 aaaaccctgc cagccagtgc gccgaaaaat ggccactata aagcttatat tctgggtgaa
    25921 gggccagatg gcgtggctaa aacaccggaa tgggcctcac aaatcaccgg tgttccggca
    25981 gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag
    26041 ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg
    26101 gcgattctga ccggtaacgt tggtattaat ggagggaaca gcggcgcgcg tgaaggttca
    26161 tacagcttac cgtttgtccg tatgccgacc ttggaaaacc cgatccagac cagcatttcg
    26221 atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt
    26281 gtacgcggga aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac
    26341 tgcctggtta accagcattc tgaaatcaac cgtacccatg aaatccttca ggatgataag
    26401 aagtgcgagc tgattgtggt tatcgactgc cacatgacct catcggcgaa atatgctgac
    26461 atcctgctgc ctgactgcac cgcttccgaa cagatggact ttgcgctgga tgcatcctgc
    26521 gggaatatgt cttacgtgat tttcaacgat caggtgatta aaccgcgctt tgaatgtaag
    26581 accatctatg aaatgaccag cgaactggca aaacgtcttg gcgttgagca acagtttact
    26641 gaaggccgta cccaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt
    26701 cctgaactgc caacgtttga agagttccgc aagcagggga tctttaaaaa gcgcgaccca
    26761 caagggcatc acgttgctta taaagccttc cgtgaagatc cgcaggcaaa cccactgact
    26821 acgccatcgg gcaaaattga gatttattcg caggcgctgg ctgacattgc cgctacctgg
    26881 gaattgccag aaggcgatgt gatcgatcca ctgccgatct acacgccggg ctttgaaagt
    26941 tatcaggatc cgctgaacaa acagtatccg ctgcagctta cgggtttcca ctataaatct
    27001 cgcgttcact caacttacgg caacgttgat gtgctgaaag cagcttgccg tcaggaaatg
    27061 tggatcaacc cgcttgatgc gcaaaaacgc ggtatcaaca acggcgataa agtcaggatc
    27121 tttaacgatc gtggtgaagt tcatattgag gcgaaagtga cgccacgaat gatgccaggt
    27181 gtggtcgcac tgggtgaagg tgcctggtat gacccggatg caaaacgtgt cgataagggt
    27241 ggttgtatta acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca
    27301 catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg
    27361 attttttatt gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga
    27421 ctacaaagat ttgacgccag aagtcagctt ccgccgcatt tatgaatatg ctggcggcga
    27481 ctggcaggaa gataacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc
    27541 gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa
    27601 acgtgaagat ggttttgttg tggtcgatga agatgtgtgc attggctgcc gctactgcca
    27661 tatggcttgc ccgtatggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg
    27721 cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg atctgtgttg aatcttgtcc
    27781 gctgcgggcg ctggatttcg gccctatcga cgagctgcgt aaaaaacatg gcgatctggc
    27841 ggccgttgcg ccgttgccgc gagctcactt taccaaaccg aatattgtga tcaaacccaa
    27901 tgccaatagc cgcccgaccg gggataccac tggctatctg gcaaacccga aggaggtgtg
    27961 agatgggaag tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg
    28021 tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgagcagaag
    28081 cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt gctgatgggc attggcttta
    28141 tcgcttctat gctccatctt ggttcaccga tgcgcgcttt taactcgctc aaccgggtag
    28201 gggcttcagc actcagtaac gaaatcgcca gcggttcgat cttttttgcc gtaggcggca
    28261 tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc attgcgtacg ctttggctgg
    28321 tggtgacgat ggttcttggc gtcgtctttg tctggatgat ggtgcgtgtg tataacagca
    28381 ttgataccgt tccgacctgg tacagcatct ggacgccgat gggcttcttc ctgacgatgt
    28441 tcatgggcgg cccgttactg gggtacctgt tgctgagtct ggctggcgtc gatagctggg
    28501 cgatgcgtct gctgccagcc atttccgtac tggcactggt ggtgagtggc gtggtgtcag
    28561 tgatgcaggg cgcagagctg gcgactattc atagttctgt gcagcaggcc gcagcgctgg
    28621 tgccggacta tggtgcgctg atgtcctggc ggatcgtgct tttggccgtt gccctgtgct
    28681 tgtggattgc accacagcta aaaggttatc agcctgcggt tccgctacta tccgtctcat
    28741 tcattctgct tctggcaggg gaattaatcg gtcgcggcgt attctatggt ttgcatatga
    28801 ccgtggggat ggccgtcgca agctaatcag taacaaccgg ggtttcggcc ccggttctct
    28861 tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa tcaggaatat
    28921 ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcacgg tgcagctcgc
    28981 aagccacgcc gaaccatgtc atcaattgcg cacctgcttg cgacatgcga tcccatgccg
    29041 aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacaacaaac acatcaaacc
    29101 cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa
    29161 taattaactg ttttttacct gtcgctttga ctgcttttac aaaatcttcg ttatcccagg
    29221 cgttaatatt gcccgggcga gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg
    29281 gaactaatgg gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga
    29341 aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat
    29401 cccgtacaag ggaaagtaaa ccagcctggt gatcaacaag caaaacagca gcatcatttt
    29461 tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgcc
    29521 tgaaatcggt cagacgtgat gcattgctct gaaagcatag acgggaaata tgagtttgct
    29581 gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttaga tcaataaaaa
    29641 caatcagatg agattaatca tctcttcagt attagcgtta tgaatgagta atataaatcg
    29701 ataataccgc cgttgcgata tgcgcgtatt ttcacccatt gacaatgttt ttggcggtgg
    29761 catgatgcgc atgaaatttg aacttcctca cggttttaat tcatgtccac gtatacccgg
    29821 cctgtcatgc ttttgctgtc tggcctgctt ttgttgactc tggcgatcgc ggtgttaaat
    29881 acactcgtgc cgctttggct cgcccaggaa cacatgtcca catggcaggt aggcgttgtc
    29941 agctcatcct attttaccgg caaccttgtc ggtacattgc tgacagggta tgtcattaaa
    30001 cgcattggtt ttaaccgcag ctattatctg gcctccttca tttttgccgc tggctgtgcc
    30061 ggccttggcc tgatgattgg attctggagt tggttggcgt ggcgttttgt cgcgggcgtc
    30121 ggctgtgcca tgatttgggt agtcgttgag agcgcgctga tgtgcagtgg gacgtcacgt
    30181 aaccgtgggc gtttgcttgc tgcatacatg atggtttatt acgtgggaac gtttttaggc
    30241 cagttactgg tcagcaaggt ctcaaccgag ctgatgagcg tattgccgtg ggttacaggt
    30301 ttgacattgg cagggatctt accgctgttg tttacgcgtg tcctgaatca gcaggctgaa
    30361 aaccatgatt cgacgtcaat tacggcaatg ctaaaactcc gtcaggcgcg gcttggcgtg
    30421 aatggctgca ttatctcagg aatcgttctg ggatctctat atggcctgat gcctctgtac
    30481 ctcaatcaca aaggggtgag caatgccagt attggtttct ggatggcggt actggtcagt
    30541 gcgggtatcc tcggacaatg gccgattgga cgtctggcgg ataagtttgg tcgactgctg
    30601 gttttgcgtg ttcaggtctt tgtcgtcatt ctcggcagta ttgcgatgct tagccaggcg
    30661 gcgatggccc cagcgttatt catcctcggt gccgctggct ttacgctata tccggtggcg
    30721 atggcatggg cttgcgagaa agttgaacat catcaactgg tggcgatgaa ccaagcctta
    30781 ctgttgagct atactgtggg aagtctgctt ggcccgtcat ttaccgctat gctaatgcag
    30841 aatttctccg ataatttatt gtttatcatg atcgccagcg tatcgtttat ctatttgctg
    30901 atgctgctgc gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta aatgaattca
    30961 agcagagtgt gaacttactg tttcacactc tgcttttttg tttcttctat ctgacttgct
    31021 ttattccaaa ttttattcgt ttaaaaataa aatgtgtagc aggttataat tttgcatttc
    31081 gctatttccg cacttcttat ttgccgcgca taatccctcg ttttacagat gcccctataa
    31141 ttttggcgaa ggatttgtct atggctggga atgttcagga aaaacagctg cgatggtaca
    31201 acattgcgct gatgtctttt atcactgtct ggggttttgg caacgttgtt aataactacg
    31261 ccaaccaggg gctggtggtt gttttttcat gggtgtttat ctttgcgctc tatttcacac
    31321 cttatgcgct aattgttggt cagttaggct cgaccttcaa agatgggaag ggcggggtca
    31381 gtacctggat taaacacacg atgggacccg gactggctta tctcgccgcg tggacctact
    31441 gggtggtgca tattccctat ctggcacaaa aaccctaggc aattctgatt gcgctcggtt
    31501 gggcgatgaa aggcgacggt tcgctaatca aagaatattc agtcgtagcg ttacaggggt
    31561 taacgctggt gctgtttatc ttctttatgt gggttgcttc acgcggtatg aaatcgctga
    31621 aaatcgtcgg ttctgtggca gggattgcta tgtttgttat gtcgctcctg tatgtggcga
    31681 tggcggtaac cgcgcctgca attactgaag tgcatattgc gaccacaaac attacctggg
    31741 aaacgttcat tcctcatatc gactttacct acattaccac tatttcaatg ctggttttcg
    31801 cggttggcgg agcagagaag atttctcctt acgttaatca aacgcgcaac ccaggaaaag
    31861 aatttccaaa agggatgtta tgcctggcgg tgatggttgc ggtttgtgcc attctgggct
    31921 cgctggcgat ggggatgatg tttgattcgc gtaatatccc ggatgactta atgaccaacg
    31981 gtcagtatta cgcctttcag aagctgggcg agtattacaa catgggtaat actttaatgg
    32041 tgatttacgc cattgcgaat accctgggac aagtagcggc gctggtattc tcgattgatg
    32101 ccccgcttaa agtgctatta ggtgatgctg acagcaaata tattccagcc agtttatgtc
    32161 gtaccaacgc ttctggtacg cccgttaatg gctattttct gaccctggta ctggtggcga
    32221 ttctgattat gctgccgact ctcggcattg gtgatatgaa caatctctat aaatggctgt
    32281 tgaaccttaa ttcggtagtg atgccgctgc gttatttatg ggtatttgtt gcatttattg
    32341 cagtcgttcg cctggcgcag aaatataaac cagaatatgt ctttattcgt aataagcctc
    32401 tggcaatgac cgtcgggatt tggtgttttg cctttaccgc ctttgcctgt ttgacgggga
    32461 tcttcccgaa aatggaagcc ttcactgcag agtggacctt ccagttggcg ctgaatgttg
    32521 caacgccgtt tgtgctggta ggattaggac tgatattccc gctgctggcg cgtaaagcga
    32581 atagtaaata attattgtgg tcggtgacgc tgactctggc agatctgacc cgtttttaca
    32641 taatcgagaa atgcgcgcaa gccgcaggac atatactggc ggtttgggta atagatctgg
    32701 aagcctggac gctctgtgat ccagtcttcc agtacacatt ctaaacgacc ggtatcaagg
    32761 tactccttga tctgttcata gagcagatac ccgataccag cacccatcaa aacggcatcc
    32821 agttcagcat caacatc
//
LOCUS       DAZPAC010000021        32701 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZPAC010000021 DAZPAC010000000
VERSION     DAZPAC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.021"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            334..1707
                     /gene="aroP"
                     /locus_tag="ACHUPM_000829"
     CDS             334..1707
                     /gene="aroP"
                     /locus_tag="ACHUPM_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR0868693.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1750..2604)
                     /gene="ampE"
                     /locus_tag="ACHUPM_000830"
     CDS             complement(1750..2604)
                     /gene="ampE"
                     /locus_tag="ACHUPM_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR0868694.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2601..3152)
                     /gene="ampD"
                     /locus_tag="ACHUPM_000831"
     CDS             complement(2601..3152)
                     /gene="ampD"
                     /locus_tag="ACHUPM_000831"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR0868695.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3240..4133
                     /gene="nadC"
                     /locus_tag="ACHUPM_000832"
     CDS             3240..4133
                     /gene="nadC"
                     /locus_tag="ACHUPM_000832"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR0868696.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4336..4776
                     /gene="ppdD"
                     /locus_tag="ACHUPM_000833"
     CDS             4336..4776
                     /gene="ppdD"
                     /locus_tag="ACHUPM_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR0868697.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4786..6172
                     /gene="gspE"
                     /locus_tag="ACHUPM_000834"
                     /pseudo
     CDS             4786..6172
                     /gene="gspE"
                     /locus_tag="ACHUPM_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            6162..7364
                     /gene="hofC"
                     /locus_tag="ACHUPM_000835"
                     /pseudo
     CDS             6162..7364
                     /gene="hofC"
                     /locus_tag="ACHUPM_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(7399..8442)
                     /gene="guaC"
                     /locus_tag="ACHUPM_000836"
     CDS             complement(7399..8442)
                     /gene="guaC"
                     /locus_tag="ACHUPM_000836"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR0868698.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8598..8642)
                     /locus_tag="ACHUPM_000837"
     CDS             complement(8598..8642)
                     /locus_tag="ACHUPM_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868699.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8667..9287
                     /gene="coaE"
                     /locus_tag="ACHUPM_000838"
     CDS             8667..9287
                     /gene="coaE"
                     /locus_tag="ACHUPM_000838"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR0868700.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9287..10030
                     /gene="zapD"
                     /locus_tag="ACHUPM_000839"
     CDS             9287..10030
                     /gene="zapD"
                     /locus_tag="ACHUPM_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR0868701.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            10040..10237
                     /gene="yacG"
                     /locus_tag="ACHUPM_000840"
     CDS             10040..10237
                     /gene="yacG"
                     /locus_tag="ACHUPM_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR0868702.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10337..10726)
                     /gene="mutT"
                     /locus_tag="ACHUPM_000841"
     CDS             complement(10337..10726)
                     /gene="mutT"
                     /locus_tag="ACHUPM_000841"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR0868703.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10786..13491)
                     /gene="secA"
                     /locus_tag="ACHUPM_000842"
     CDS             complement(10786..13491)
                     /gene="secA"
                     /locus_tag="ACHUPM_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR0868704.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13553..14065)
                     /gene="secM"
                     /locus_tag="ACHUPM_000843"
     CDS             complement(13553..14065)
                     /gene="secM"
                     /locus_tag="ACHUPM_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR0868705.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14296..15213)
                     /gene="lpxC"
                     /locus_tag="ACHUPM_000844"
     CDS             complement(14296..15213)
                     /gene="lpxC"
                     /locus_tag="ACHUPM_000844"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR0868706.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15314..16465)
                     /gene="ftsZ"
                     /locus_tag="ACHUPM_000845"
     CDS             complement(15314..16465)
                     /gene="ftsZ"
                     /locus_tag="ACHUPM_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR0868707.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16526..17788)
                     /gene="ftsA"
                     /locus_tag="ACHUPM_000846"
     CDS             complement(16526..17788)
                     /gene="ftsA"
                     /locus_tag="ACHUPM_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR0868708.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17785..18615)
                     /gene="ftsQ"
                     /locus_tag="ACHUPM_000847"
     CDS             complement(17785..18615)
                     /gene="ftsQ"
                     /locus_tag="ACHUPM_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR0868709.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18617..19537)
                     /gene="ddlB"
                     /locus_tag="ACHUPM_000848"
     CDS             complement(18617..19537)
                     /gene="ddlB"
                     /locus_tag="ACHUPM_000848"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0868710.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19530..21005)
                     /gene="murC"
                     /locus_tag="ACHUPM_000849"
     CDS             complement(19530..21005)
                     /gene="murC"
                     /locus_tag="ACHUPM_000849"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR0868711.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21059..22126)
                     /gene="murG"
                     /locus_tag="ACHUPM_000850"
     CDS             complement(21059..22126)
                     /gene="murG"
                     /locus_tag="ACHUPM_000850"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR0868712.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22123..23367)
                     /gene="ftsW"
                     /locus_tag="ACHUPM_000851"
     CDS             complement(22123..23367)
                     /gene="ftsW"
                     /locus_tag="ACHUPM_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR0868713.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23367..24683)
                     /gene="murD"
                     /locus_tag="ACHUPM_000852"
     CDS             complement(23367..24683)
                     /gene="murD"
                     /locus_tag="ACHUPM_000852"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR0868714.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24686..25768)
                     /gene="mraY"
                     /locus_tag="ACHUPM_000853"
     CDS             complement(24686..25768)
                     /gene="mraY"
                     /locus_tag="ACHUPM_000853"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR0868715.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25762..27120)
                     /gene="murF"
                     /locus_tag="ACHUPM_000854"
     CDS             complement(25762..27120)
                     /gene="murF"
                     /locus_tag="ACHUPM_000854"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR0868716.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27117..28610)
                     /gene="murE"
                     /locus_tag="ACHUPM_000855"
     CDS             complement(27117..28610)
                     /gene="murE"
                     /locus_tag="ACHUPM_000855"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR0868717.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28597..30363)
                     /gene="ftsI"
                     /locus_tag="ACHUPM_000856"
     CDS             complement(28597..30363)
                     /gene="ftsI"
                     /locus_tag="ACHUPM_000856"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR0868718.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30379..30744)
                     /gene="ftsL"
                     /locus_tag="ACHUPM_000857"
     CDS             complement(30379..30744)
                     /gene="ftsL"
                     /locus_tag="ACHUPM_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR0868719.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30741..31682)
                     /gene="rsmH"
                     /locus_tag="ACHUPM_000858"
     CDS             complement(30741..31682)
                     /gene="rsmH"
                     /locus_tag="ACHUPM_000858"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR0868720.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31684..32142)
                     /gene="mraZ"
                     /locus_tag="ACHUPM_000859"
     CDS             complement(31684..32142)
                     /gene="mraZ"
                     /locus_tag="ACHUPM_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR0868721.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         7781 a         9172 c         8052 g         7696 t
ORIGIN      
        1 taacgattat taaactataa aaatccgaat tgaaccgatt cacttaccaa ttttttgatt
       61 tttaagtcaa taaaaacgaa tttaaattca ttctgcatat tgagaggggt tgaggctgag
      121 ctttacaaac ggtttctttt taagcaactc atcttcaacc atgcataaag cgggtgcatt
      181 cgctgccgca taccattatt cttgatctga cggaagtctt tttgtaacaa ttcaaacttc
      241 tttgatgtaa aaaaattaat acaacaaacg gaattgcaaa cttacacacg catcactgcg
      301 tagatcaaaa aaacaatcac cgcacgaggt ttcatgatgg aaggtcaaca gcacggcgag
      361 cagctaaagc gcggccttaa aaaccgccat attcagctta tcgcgctggg tggcgcgata
      421 gggacagggc tattcctggg tagcgcctcc gtaatacagt ccgcagggcc agggattatc
      481 ctgggttacg ccattgctgg ttttatcgcc tttctgatca tgcgtcagct gggtgaaatg
      541 gtggtcgaag aacctgtcgc aggctccttt agccactttg cttataaata ctggggcagt
      601 tttgccggtt tcgcctctgg ctggaactac tgggtactgt acgttttagt tgccatggca
      661 gagctgactg ccgtgggtaa atacattcag ttctggtatc cggaaatccc aacctgggtt
      721 tctgccgccg tattctttgt ggtgattaac gccatcaacc tgaccaacgt taaagtgttt
      781 ggcgagatgg agttctggtt tgccattatc aaagttattg cgatagtagc gatgatcatc
      841 ttcggcggct ggctgctgtt cagtggcaac ggcggcccgc aggcgagcgt tagcaacctg
      901 tgggatcaag gtggtttcct gccgcacggc ttcaccggac tggtgatgat gatggcgatt
      961 atcatgttct cgttcggtgg tctggaactg gtggggatca ccgcagcaga agctgataac
     1021 ccggagcaaa gtatcccgaa agcgactaac caggttatct accgcatcct gattttctat
     1081 attggttcgt tagccgttct gctctcactg atgccgtgga cccgcgttac cgccgatacc
     1141 agtccgtttg tgctgatctt ccacgagtta ggcgatacct ttgtggcgaa tgcgctgaac
     1201 atcgtggtac tgactgcggc gctctccgtg tacaacagct gcgtatattg caacagccgt
     1261 atgctgtttg gtctggcaca acagggtaat gcgccaaaag cgctggcgtc tgtcgataaa
     1321 cgcggcgtac cggttaacac cattctggtg tctgcgctgg ttacagcatt gtgcgtattg
     1381 attaactatc ttgctccgga atccgcattt ggcctgttaa tggcactggt ggtatccgca
     1441 ctggtgatca actgggcgat gatcagtctg gcgcatatga agttccgtcg cgccaagcag
     1501 gaacaaggcg tggtaactcg cttccctgct ctgctttatc cgctgggtaa ctggatctgc
     1561 ctgctgttta tggcggcggt actggtgatt atgctgatga ccccaggaat ggcgatttcg
     1621 gtatacctga tcccggtatg gctgatcgtg ttaggtatcg gctatctgtt taaagagaaa
     1681 accgccaaag ccgtaaaagc gcattaatct ctctacgccc tcacccatgc agggtgaggg
     1741 caataatctt tacaccaacg ctccgtaaat cgtcagtagc gcaatcacca ccacgaccac
     1801 gaacgaggtt ttcttcgcca ttgaaaccgc tgccttcggc gtctccacct tatcgacatg
     1861 cggttcacgc gccagagaga actgcgccag acgcgttaac acctgatact gcgaagtatg
     1921 gaagtcaccc agcgaagcaa accaggccgg taacgctttc tccccatgac cgatcaaggc
     1981 atataccaca cccgcaagac gaaccggcac ccaatccagt acatgaagca cggcatcaat
     2041 gccggactgt aaacgatgat gcggcgtctg gtatcgcgcc agccagtatt gccatgcacg
     2101 caaaaacgca taccccatca gcgtaacggg tccccaggtt ccccccacaa tcaaccagaa
     2161 cagcggtgca agataaaaac gaaagttaat ccacagcaat gcattttgca gctcacgcaa
     2221 atactcacgt tcgtcgcagc ctgccgggac gccgtgaatc atggtgagtt cgccagccat
     2281 cgtggcacgg gcatggctat cattacgtga agcagctgtc agataagcat gataatgaag
     2341 acgaacttta cctgcgccaa tacacagcaa accaatcagc agccacacca gcagcgtggg
     2401 aacgttgaac aatactccct gcaatgcgcg taacagtaaa aaagtcacgc ccatcgcaat
     2461 aatggtcatg cctaacgtgc gcccgagaga aaaatgtttc acccgccgaa agaacgcttc
     2521 aagacgatga tcaagctgcc agtgctcgcc caacttaaac aggcgctcga aaattaacac
     2581 cagtaaggtt gtaaatagcg tcatgttgtc tccttgctga ccagcgcacg aaaccgtgcc
     2641 caatcaaatg caggaccggg atcggttttc cgatccggcg caatatcaca atggcccgtc
     2701 atgttattag cgatatcggg atagcgatct atcagtgccc gcgtaaccgc cgcaagctgt
     2761 tgatactgcg catcggtata cgccagagta tcggtgcctt caagctcaat cccaatagaa
     2821 aaatcattgc agcgttcacg cccctgatac tgagagactc ccgcatgcca ggcacgttta
     2881 tcgaaaggaa catactggac tatttcacca tcacggcgaa tcaaacagtg agcggagacg
     2941 cgcaaatggg cgatctcagc aaagaaagga tgtgcctgcg gatcaatagt tccagtgaat
     3001 aatgcgtcga tccacggacc gccaaactcg cctggcggca ggctaatatt gtgcaccacc
     3061 agcagggtgg gtgtttcgtc atccgggcgg caatcgtaat gtggtgaggg aacgcggcgc
     3121 gcgccaacca gccacccctg ttctaacaac atgcaggatc tccttatatg tggtgctaat
     3181 acccggttca gagtagcatg tttctacctt atgattcgtt agctatctgg agttttaaca
     3241 tgccgcctcg ccgctataac cctgacaccc gacgtgacga gctgctggaa cgcattaatc
     3301 tcgatatccc cggcgcggtg gcccaggcgc tgcgggaaga tttaggcgga acagtcgatg
     3361 ccaacaatga cattacggca aaacttttac cggaaaactc tcgctctcat gccacggtga
     3421 gcacccgcga gaatggcgta ttttgcggca aacgctgggt tgaagaggtg tttattcaac
     3481 tggcaggcga cgatgtcacc ataatctggc atgtggatga cggcgatgtc atcaacgcca
     3541 atcaaccctt gttcgaactt gaaggcccat cccgcgtgct gttaacgggc gaacgcactg
     3601 cgcttaattt tgtgcaaacc ctttcaggag ttgccagtaa ggtacgccac tatgtcgaat
     3661 tactggaagg caccaacacg cagttgttgg atacgcgcaa aaccttacct ggcctgcgtt
     3721 cagctctgaa atacgcggta ctttgcggcg gcggagcgaa tcaccgtctg gggctttctg
     3781 atgccttcct gatcaaagaa aatcatatta ttgcctccgg ctcagtgcgc caggccgtcg
     3841 aaaaagcgtc ctggctgcac ccggatgcgc cagtagaagt cgaagtagag aatctggaag
     3901 aacttgatga agccctgaaa gcaggagccg atatcatcat gctggataac ttcgaaacag
     3961 aacagatgcg cgaagccgtc aaacgcacca acggcaaggc gctactggaa gtgtctggca
     4021 acgtcactga caaaacactg cgcgaatttg ccgaaacggg cgtggacttt atctccgtcg
     4081 gtgcgctaac taaacacgta caagcactcg acctttcaat gcgttttcgc taattgctga
     4141 cagatttatc cgctccatcg cgggcggata atctcccctt ccccaacttt cttcgtaacg
     4201 cctcgcaaat ttcttgtctt ttgctgcaac gaaataaaaa acctcggaag ctgctttccc
     4261 gtttcgtttt ttgcccctcg cttgtgctga tggcgtttgc caaagtagcg ccaaccaaat
     4321 caaggagcga aacagatgga caagcaacgc ggttttacac ttatcgaact gatggtggtt
     4381 attggcatca ttgccatttt aagcgccatt ggtattcccg cttatcaaaa ctacctgcgc
     4441 aaagctgcac tcaccgacat gctacaaacc tttgtgcctt accgtacagc cgtagagttg
     4501 tgcgcgctgg aacatggtgg attagatacc tgcgacggtg gcagcaatgg cattccctcg
     4561 cctaccacca cccgctatgt ttcagccatg agtgtggcaa aaggcgtggt gtcgctgacc
     4621 gggcaagaaa gtctcaatgg actaagcgtc gtcatgacac cgggttggga taacgcaaac
     4681 ggcgtcaccg gctgggcgcg caactgcaat attcaaagtg acagcgcatt gcagcaagcc
     4741 tgcgaagatg tcttccgctt tgatgacgcc aactaaggag cggcaatgaa tattccacag
     4801 ctcaccgccc tgtgtctgcg ttatcaggga gtcttgctgg atgccagcga agaggtggtt
     4861 catgttgcgg tagtcgatgc accttcgcat gagttgctgg acgcattgca tttcgctacc
     4921 accaaacgta ttgagatcac ctgctggacg cgccaacaaa tggaaggcca cgccagtcgc
     4981 acacaacaga cattgcccgt agctgttcag gagaagcatc agcccaaagc agagttgctg
     5041 actcgaacgt tacaatctgc gctggaacaa cgtgcgtctg atattcatat cgaaccagcg
     5101 gacaatgcct accgcatccg cttgcgtatc gacggcgtat tgcatccttt accggatgtt
     5161 tcaccggatg ccggagtcgc attaaccgcc agattaaaag tgctgggaaa cctggatatt
     5221 gcggaacatc gcctgccgca ggacgggcaa ttcactgtcg aactggcagg aaacgccgtc
     5281 tcatttcgta ttgcgacctt accatgtcgg ggtggtgaaa aggtggtatt aaggttgtta
     5341 cagcaggtga accaggcact ggatgttaac acgctgggaa tgcagccgtt acaactggcg
     5401 gactttgctc atgccttgca acaaccacag ggactggtgc tggtaactgg ccctacaggc
     5461 agcggcaaaa cggtcacgct ttatagtgcc ctgcaaaagc tgaataccgc tgacattaat
     5521 atttgtagcg tcgaagatcc ggttgagatc cccatagccg gactaaacca gacgcaaatc
     5581 catccgcgtg ccgggctcac ctttcagggc gttttgcgtg cgttattgcg ccaggatcct
     5641 gacgtcatca tgatcggaga gatccgctat ggctttaacg gcagaaattg ccattaaagc
     5701 cgcgcaaacc ggtcacctgg tgttgtctac cctacacact aattccacct gcgaaacgct
     5761 ggtacgttta cagcaaatgg gagtcgcccg ctggatgctc tcatcagcgc ttacgctggt
     5821 aatagcccag cgtctggtac gtaaactttg cccacattgt cgccagcagc aaggggagcc
     5881 catccatatt ccagtcaatg tatggccgtc gccgctgccc cactggcagg cacccggttg
     5941 tgtacattgc taccacggtt tttatggtcg tacggcctta tttgaagttc tgcccataac
     6001 gccggtcatt cgtcagctta tttccgctaa taccgacgtt gaatcgctgg aaacgcacgc
     6061 acgacaggcg ggtatgcgta cgctttttga aaacggctgc ctggccgtgg agcaaggctt
     6121 aaccaccttt gaagagttaa tccgcgtact ggggatgccg catggcgagt aagcaactct
     6181 ggcgctggca tggcataacc ggcgacggca atgcgcaaga tgggatgcta tgagcagaga
     6241 gccgtgcttt gctgctcatg gcactacagc aacagatggt taccccactt agcctgaagc
     6301 gaatcgccat caattctgcg cagtggcgag gagataaaag cgcggaagtc attcatcaac
     6361 tggcgacgct actcaaagcc gggttaacgc tttctgaagg gctggcactg ctggcggaac
     6421 agcatcccag taagcaatgg caagcgttgc tgcaatcgct ggcgcacgat ctcgaacagg
     6481 gcattgcttt ttccaatgcc ttattaccct ggtcagaggt atttccgcca ctctatcagg
     6541 cgatgatccg cacgggtgaa ctgaccggta agctggatga atgctgcttt gaactggcgc
     6601 gtcagcaaaa agcccagcgt cagttgaccg acaaagtgaa atcagcgtta cgttatccca
     6661 tcatcatttt agcgatggca atcatggtgg ttgtggcaat gctgcatttt gttctgccgg
     6721 agtttgccgc tatctataag accttcaaca ccccactacc ggcactaacg caggggatca
     6781 tgacgctggc agactttagt ggcgaatgga gctggctgct ggtgttgttc ggctttctgc
     6841 tggcgatagc caataagttg ctgatgcgcc gaccgacctg gcttatagtg cggcagaaat
     6901 tgctgttacg catcccgatt atgggttcac tgatgcgggg acaaaaactc acgcagatct
     6961 ttacgattct ggcgctgaca caaagtgcag gcattacttt tttgcagggc gtagagagcg
     7021 tcagagaaac aatgcgctgc ccgtactggg tgcaacttct gacacaaatc cagcacgata
     7081 tcagtaacgg tcatcccatc tggctggcgc taaaaaatac cggggagttt agcccgctct
     7141 gtttgcaatt agtgagaaca ggagaggcat ccggctcgct ggacctcatg ttagacaacc
     7201 tcgcccatca tcatcgggat aacacaatgg cgctggcgga taacctcgca gccttactgg
     7261 aaccgacgtt gctgatcata acgggaggaa ttatcggtac gctggtggtg gcgatgtatc
     7321 tgccaatttt ccatttaggc gatgcgatga gtgggatggg ataacgctgg cgtgttgctc
     7381 cacaccagcg ttggaagatt acaggttgtt gaagatgcgg ttttcttgtt cctgcacacg
     7441 gataaatgtg gtgcgcttgg tcagttcttt caggcgtgaa gccccaacgt atgtacaagc
     7501 tgaacgcagg ccgcccaaaa tatcacgcgc ggtattttca accgggcctc gcagcggcag
     7561 cttaacggtt ttaccttctg ctgcgcgata ttccgcaacg ccgccaacgt gacgtttcat
     7621 cgcagactcg gagctcatgc cgtagaacag cataaatttc tcgccgttct cctcaacgat
     7681 gcgaccgccg ctctcttcgt ggcccgccag catgccgcca agcatgacga aatcggcacc
     7741 gccgccaaag gcttttgcga catcgcccgg cgtggtgcag ccaccgtcgc tgacgatcat
     7801 tccgcccaga ccgtgcgccg catcggcaca ttcgattacc gcagaaagct gcggataacc
     7861 gacgcctgtt ttgacgcgag ttgtacaaac agaacctggg ccaatgccaa ctttaacgat
     7921 atcagcaccg gagaggataa gctcctcaca catttcacca gtcactacgt tgccagcaca
     7981 aatggttttg gtcggccacg cttcacgcgc tttcgcaacg aactgcacga agtgttcgga
     8041 ataaccattc gccacatcaa tacaaacgaa gtttaatgcc gggttcaggt cgagaatctg
     8101 tttagttttt tcgaaatccg catcagacgt accggtagaa accatcacat gtttcagcac
     8161 atcagcggaa gaattgttga taaacgcttg ccactcttcg acagaatagt gtttatgcac
     8221 agcagtcaaa atatcaaaag aagccagcgc agaggccata gaaaatgtac ctacggtgtc
     8281 catatttgcg gcgataatcg gcacgccgga ccagctctga cctgaatgtt tgaaggtgaa
     8341 ttgacgttcc agttcaacat cggaacggct tttaagagtg gagcgtttag ggcggatgag
     8401 aacgtcttta aaacctaact tcagatcttc ttcaatacgc atgtgcggat tcctggggtt
     8461 aatggctata agggtaagag cgcaactcca gtgacgctat catacgcact aatcaatgcg
     8521 gcgcaagact gcgaaattgt ctttttttac gctacaatcc cataaattta cctggtaaat
     8581 agtgagcgaa cgtttcatca atttcgcttt cgtgcactca tttttaacgt tttgataatc
     8641 atgatgaatt cccgggaata taaattatga ggtatatagt tgccttaacg ggaggcattg
     8701 gcagtggcaa gagtaccgtt gccaatgcgt ttgctgatct cggaattaac gtcattgatg
     8761 ccgatattat tgcgcgtcag gtggttgaac caggtgcacc tgcgctacat gccattgctg
     8821 atcactttgg cgctaacatg attgctgctg atggaacatt gcagcgccgg gccttgcgcg
     8881 agcggatctt cgccaacccg gaagagaaaa actggcttaa cgccctgctg catccgctga
     8941 ttcagcaaga gacgcaacac cagatccagc aagctacttc cccctatgta ctgtgggttg
     9001 tgccattgct ggtagaaaac tcactgtata aaaaagcgaa tcgagtgctg gtggtggatg
     9061 tcagcccaga aacgcaactt aagcgcacca tgcagcgcga tgatgtaact cgcgagcatg
     9121 tcgaacaaat ccttgctgct caggcaacgc gcgaagcccg ccttgccgtg gcagatgacg
     9181 tcattgataa taacggcgca ccggatgcta tcgcatcgga tgttgcccgc ctgcacgcac
     9241 actatttgca gcttgcgtcg cagtttgtct cacaggaaaa accgtaatgc agacccaggt
     9301 cctttttgaa catccactaa atgaaaaaat gcgtacatgg ctgcgcattg agtttttgat
     9361 tcagcaactc accgttaatt tacccatcgt tgaccacgct ggcgcgctgc atttcttccg
     9421 taatgtcagt gaattactgg atgttttcga gcgcggcgaa gtccgcactg agctgttgaa
     9481 agaacttgac cggcagcaac gtaaactcca gacctggatt ggcgtgcctg gcgtggacca
     9541 gagccgtatt gaagcattaa ttcagcagtt aaaagcggcg gggagcgtat taatttccgc
     9601 gccgcgtatc gggcaatttc tgcgtgaaga tcgtttgatt gctctggtgc gtcagcgact
     9661 gagcatccca ggcggctgtt gcagctttga tttacctaca ttgcacattt ggctgcatct
     9721 accccaggcg cagcgcgaca gccaggtaga aacctggatt gccagcctga acccgctcac
     9781 ccaggcactt accatggtgc tggatttaat tcgccagtcg gcccccttcc gtaaacaaac
     9841 cagtctgaat ggtttttatc aggataacgg tggcgatgcc gacttgctgc gcctgaatct
     9901 gtcgctcgat tcacagcttt atccgcaaat ttccggacat aagagccgtt ttgccattcg
     9961 ttttatgccg ctggacactg aaaacggaca ggtaccggaa cgtctggatt tcgaactggc
    10021 ctgttgctaa ggagtaaaaa tgtcagaaac tattacggtg aattgcccaa cctgcgggaa
    10081 aacggtggtg tggggtgaaa tcagcccgtt tcggccattt tgctccaaac gttgtcagct
    10141 gatcgacctc ggagaatggg ctgctgaaga aaaacgaatc cccagcagtg gcgatctttc
    10201 cgaaagcgat gactggagcg aagagccaaa gcagtgacat ttgtctgatg ccgcacgtag
    10261 gcctgataag acgcggacag cgtcgcatca ggcatcttgt gcaaatgtcg gatgcggcgt
    10321 gaacgcctta tccgacctac agacgcttaa gcttcgcaat taccggttca ttggctggcg
    10381 gaaaatcatc ggcattaaga ccgactagcg acatccactc accgggttgc ccttctttac
    10441 cccacggctc cccttcccag ctttcgacca gccagaacca cagagttata tgcctgtccg
    10501 ggaattcata ttccagtttt tcaaatagcg aaaaatgttg tggggtaatc ccgacttctt
    10561 cctgaagttc acgcactacc gcctgttccg gcgtttcgcc catttcaatt ttaccgccgg
    10621 gaaactccag tttattcgcc atgtgcgcat ctgctgcgcg acgcgttata aagatttcat
    10681 tgttctcgtt gcgaataata cctaccgcaa tttgcagctt tttcattgtc ttaaacctat
    10741 aaaaaaggcg cagaatcctg cgccttttac ttcaactgtt agcttttatt gcaggcgacc
    10801 atggcactgc ttgtattttt taccagaacc gcacgggcaa ggatcgttac gtcctacttt
    10861 gcgctcaccg gtttgcgccg ccagtgcagc tgcggctgca gagtcgtcat cctgatggct
    10921 aagctgctgc atttgcgcta aacgctcggc ttccatacga cgctgttgtt ccagctcctc
    10981 aacctcttca ggcatacgta cctggacttt gctcagcgtg ctgataactt catattttaa
    11041 cgactccagc atcgctgcaa acatggagaa cgattcacgt ttgtattcct gcttcggatc
    11101 tttctgtgca tagccacgca ggtggatacc ctgacgcaga tagtccatcg ccgctaggtg
    11161 ctctttccac agagagtcga gagtttgcag catgacgcct ttttcgaagt gacgcatcat
    11221 ctcagcacca accacttctt ctttacgctg atacacttcg atggactgcg ccagaattcg
    11281 ctcacgcagc gtctcttcat gcagttctgg ttctttatcc agccactcgg caattggcaa
    11341 atcgaggtcg aaatcgttct tcagacgttc ctgcagcccc ggaatatccc acatttcttc
    11401 cagtgactgt ggcggaatgt aggcatcaat ggtcgctttg aacacatctt cacgaatgct
    11461 gttgatggtt tcgctcacat cgctgacatc cagcagttcg ttacgctggg agtaaatggc
    11521 gcgacgctga tcgttagcca cgtcatcata ttccagcagt tgcttacgaa tgtcgaagtt
    11581 acggctttca actttacgct gggcgttggc aatcgcttta gtcacccacg ggtgttcaat
    11641 ggcttcgcct ggcttcatac ccagtttacg catcatgccg gatactcggt cggaagcaaa
    11701 aatacgcatc agcgcatctt ccatcgacag gtagaaacgg gaagaaccag catccccctg
    11761 acgaccagaa cgaccgcgca actggttatc gatacgacgg gattcgtgac gctcggtacc
    11821 gatgatatgc aggccacctg cttccagtac cgcatcgtga cgtacctgcc agtcggcttt
    11881 aattttttca atttgctctg cggtcggatt ttccagcgcg gcaacttctg cctgccagct
    11941 accaccgagc acaatatctg taccacgacc cgccatattg gtcgcgatag tcaccgcagc
    12001 cggataacct gcctgagcaa caatcgccgc ttcgttggcg tggaatttgg cgttcaggac
    12061 gttgtgctta ataccggctt tggtcagttc gtttgacacc agctccgatt tttcgatgga
    12121 gatagtaccc accagcaccg gctggccttt cgcagtacgt tctttgatat cttcaatgat
    12181 cgcctgaatt ttttccgctt cagtcatgta gaccaggtcc ggcagatctt tacgaatcat
    12241 tggacggttg gtcggaacaa cgacggtatc cagcttgtag atggagctaa attcgaaagc
    12301 ttcggtatca gcagtaccgg tcatccccgc cagtttttca tacagacgga agtagttctg
    12361 gaaggtgatc gaagccagcg tttggttttc gttctggatc tgcacacctt ctttcgcttc
    12421 cacagcctgg tgcagaccat cggaccagcg acggccctgc atggtacgac cggtgtgttc
    12481 gtcaacgatg ataacttcac catctttaac gatgtagtcg acgtcgcggg taaacagcgc
    12541 atgagcgcgc agcgccgccg ttacgtggtg catcagcatg atgttggccg gagagtacag
    12601 agactcccct tcatccatga tgccctcttt caccagcagt tcttcaatca gcaccagacc
    12661 acgttcggtc aggttcacct ggcgagattt ttcgtccacc gagaagtggc cttcgccctg
    12721 gaaggtttcg gagtcttctt tttcctgacg gatcaggtgc ggaataattt tattcacgcg
    12781 tttatacatt tccgagctgt cttctgccgg gccggaaatg atcagcggtg tacgcgcttc
    12841 atcgatcagg atggagtcca cttcgtccac cagcgcatag tgcagtttac gctgtacacg
    12901 ttcttcaggg ctgaacgcca tattgtcgcg caggtagtca aagccgtatt cgttgttcgt
    12961 accgtaagtg atgtcagctg cgtaagcttc gcgctttgcc ggtgctggca tgcccggcag
    13021 gttgataccg acagtcaggc caaggaattc aaacagcgga cggttgtttt cggcgtcacg
    13081 ttgcgccagg tagtcgttga cggtaactac gtgcacgcct ttaccggtta gtgcgttcag
    13141 gtaagcaggc agcgttgcgg tcagggtttt accttcaccg gtacgcattt cggcgatgca
    13201 gcgttcgtta agaaccatac cgccgagtaa ctgaacgtcg aagtgacgca taccaaagac
    13261 gcgcttactt gcctcacgta ccacggcgaa agcttccggg atcagatttt ccagcacttc
    13321 gcctttttcc agacgtgcac gaaactctgc ggttttccct ttcagttctt cgtcggagag
    13381 tttttccatc tccggttcca tggcattgat gatgttgacc actttgcgca tccggcgcag
    13441 ggtgcgatcg ttacgactac cgaaaacttt agttaacaat ttgattagca taataaaatc
    13501 tcaaacgccc cgcgttgcgg agttaataaa atgaagtaaa ggtttattgt tgttaggtga
    13561 ggcgttgagg gccagcacgg atgccttgcg cctggcttat ccagacgggc gtgctgaatt
    13621 ttgcttgtgg ggtaaaatgc gcataatcaa tgcgataacc cttttcagac ggcgtgcctt
    13681 cctgggtcag cagcgcgctg agcgtatcca gtaatgcaag atgttgcgcc tgaagaggca
    13741 aagactcttc agcaacgggc agtgtttgcg gtgccattgc gaaagaaaga tgacggatta
    13801 ccgtgcgaat ggcatgttga tgccagtaat caacggaata gttcgaattc gggcggcgtg
    13861 tgttcgcttc cagcaaggcc aattgaccaa agttaacttt ggctgaaggc tcgtggttgc
    13921 gggttgtcgc ttttgcgggc gcgtttggtt cggcggcgtt gctgagcgca ggcaaaccta
    13981 aactcgccgc aaccatccct aataagagat gcggccagaa gtagcgttta ccaaactgtc
    14041 gccagcgcgt cagtattcca ctcacgttat tgccatcccg atcgtattca aacgtattta
    14101 gcgcacaaat cttatcatta aagcctgcag tccacagcac gaatgaagat aagttgtgcg
    14161 gaaaagtgct taagaaaaca gcgttcgcac cgttttttat agctcttata aaattcaaaa
    14221 aaaaatgact ggttcacctg gccggagaga gtgccagatt tgccagtcga attttatacg
    14281 acagtataaa tgtcgttatg ccagtacagc tgaaggcgct ttgaaggcca acggcagttc
    14341 tgcgtcgtcc tggaaggtca catattccca ggcttcctgt ttcgccagga cagcctgcag
    14401 cagtttgtta ttcagtgcat gaccggattt ataagcggta aatgcaccaa taatattgtg
    14461 accacacatg aacaagtcac cgatcgcatc gagcattttg tgacgcacaa attcgtcttc
    14521 aaaacgcagg ccgtcttcgt tcagtacgcg ataatcgtca acaacgatgg cacaatcgaa
    14581 gctgccgccc aggcacaaac cacgggactg cagatattcg atatcacgca tgaaaccgaa
    14641 cgtacgcgca cggctgatct ggcgcataaa cgcatcagcg gagaagttca tcgcatagcg
    14701 ctggttgctg gaatcaatag ccggatggtt aaaatcgatg gtgaaatcca gcgaaaaacc
    14761 attgtacggc ttaaattcag cccacttatc gccatcttcg acacgaacag tctctttgat
    14821 gcgaacaaat tttttggcgc agttcaactc gtcgataccg gcgtcaagca gcaggtatac
    14881 aaacggagcg gcgctgccgt ccatgatcgg gatttccggc gcgttaactt cgataacaat
    14941 gttatcgatg cccaggcccg cgagagcagc attgaggtgc tctacggttg aaatccgtac
    15001 atcatgctcg ttgaccagac acgtacagag catggtatca cgcacagatt tggcatcggc
    15061 cgggaaatct accggtggat tcaagtcggt gcgacgatag atgaccccgg tgttggccgg
    15121 cgcagggcgt aacgtcagag tgactttctt gccggtatgt aaaccgacac ccgtcgcctg
    15181 aacgatacgt ttaagtgtcc tttgtttgat catcgtatta tctcgccaaa ttacctatcc
    15241 aaccgaagtg tactatacat tcggcgggcc agtttagcac aaagagcctc gaaacccaaa
    15301 ttccagtcaa ttcttaatca gcttgcttac gcaggaatgc tgggatatcc agataatccg
    15361 gctctttcgc agtttgcggc gcattgtcat tcacgacttt agcaaccggc ttctgctcct
    15421 gggtcagcgg agccatccca tgctgctggt agcgatccat cactggctgc tgaacctgct
    15481 tattggtcac cagagtgatt tcaggacgtt tgtccatgcc gatacctgtc gcaacaacgg
    15541 ttacgcgcag ctcgtcattc atatccgggt caagagaagt accgataacc acagtcgcgt
    15601 tgtcggaagc aaatgcacgg atggtgttac ctaccgtttc gaactcatcc agacgcaggt
    15661 cgaagcccgc cgtgatgtta accagcacgc cgcgcgcgcc agacaggtcg atatcttcca
    15721 gcagcggaga agagatagcc atttcagcag cttcttccgc acggtcttca ccgctcgcca
    15781 cgccagaacc catcattgcg tagcccatct cagacattac ggtgcgtacg tctgcaaagt
    15841 ccacgttcat caaacccgga cgagtaatca gttcagcgat accttgcaca gcgcctttca
    15901 gtacatcgtt cgctgcgcca aacgcatcca gcagggagat accgcggccc agaactttca
    15961 gcagtttgtc gttcgggata gtgatcagag agtccacatg cttggacagt tcagtgatcc
    16021 cctgctccgc gaatgccata cgcttcttgc cttcaaagtt gaaaggctta gtgacgacag
    16081 caacggtcag gatacccaaa tcttttgcca cttcagcgac gactggtgct gcacctgtac
    16141 cggtaccacc acccataccc gcagcaataa agaccatgtc tgcaccttcc agcgccgcac
    16201 gcaatgcatc gcgatcctca tcagccgcat tgcgaccaac ttctggatta gcgccagcgc
    16261 ccagtccttt ggtgataccg ctaccgattt gaatcgtctg tccaaccgct gttttacgca
    16321 gcgcttgtgc atcggtattt accgcgaaga attcaacacc ttcaatacgc tcgcgcacca
    16381 tgtgttcaac agcattaccg ccgccgccgc cgacgccgat gactttaatc accgcgtcat
    16441 tggtaagttc cattggttca aacatagttt ctctccgatt tgtgcctgtc gcctgaggcc
    16501 gtaatcatcg tcggcctcat aaaaattaaa actcttttcg cagccaacta ttgagtcgct
    16561 tgatccacga gccaactgat gctgtaacac gtttttctac ttcagcttca ccgttaagat
    16621 gtgactcttt cccatagtga agcaatccca ccgccgtcga ataatacggc tcctgagcat
    16681 aatccgttaa accggtaatg ttcagcggcg cgccgatacg cacttgcgta tgaaacacgc
    16741 gctgagcaca ggctgcaaga ccttcgatct gcgctgcgcc accggttaat acaatgcctg
    16801 ccgccaggtg atgtttaacc ccttgttggc gaagcttttc ctgcaactgc aatatctctt
    16861 cgttgaccag gttgagcagc tcggtatagc gcggctcgat cacctctgcc agggtctgac
    16921 gttgcagact ccgtggcgga cgaccgccta cgctcggcac ttccacgctc tcatcttttc
    16981 caacgatgga acccagcgca caaccgtggc gaactttaat cgcttcggcg tcgcttggcg
    17041 gcgtgccaaa ggcgtaagcg atatcactgg tcacgacatt gccagcataa ggaattacct
    17101 tagtgtggcg caatgcccca ccggtataaa cggcgatatc cattgtacca ccaccgatat
    17161 cgacgacgca gacacccagt tcacgttcat cttccgtcaa taccgaataa cttgatgcca
    17221 gtccggcaaa tatcagttgg tcaactttca gcccacaacg ttcaaccgct ttgacgatgt
    17281 ttttcgccat atcgttgtga catgtgatca ggtgcacttt tgcctgcatc cgcacgcccg
    17341 aaagtcctac cggattcttg atcccttcct gatagtcaat cgcatactct tgcgggatca
    17401 catgcagcac acgatgttca tcgcgcacac gcaccgattt cgcggtatgg acgacgtttt
    17461 ccacatcttc ttgcgtcact tcttcttcag aaataggcac cataccaatt tcattctggc
    17521 agctgatgtg cttaccagaa agcgccagat ataccgaaga gatctgacaa tctgccatca
    17581 attctgcctg gtcaatggcg cgttgtacgc acttgaccac ggattcgagg tcgttcaccc
    17641 cgcctttatc cataccacgt gacgggcagc tgcccacgcc aatgatattg accataccgt
    17701 cgggcagaac ttcccctact aaagcggcaa ccttcgcggt accaatctcc agtcctacta
    17761 ccagttttct gtccgtcgcc ttgatcattg ttgttctgcc tgtgcctgat tttgttgctg
    17821 agtagattcc tctggcggca agggcgccca gcctactgcc gctccagagt cataacgcaa
    17881 atcaacgtag ctaatccgtt tgccatcggt ttgcgcctgc tgctgtaaaa ccggataaag
    17941 ttctacaaag cgagccaaac gtttcatcgt atcgccccgg ccaagattga gcttaatatc
    18001 gttattcagc gtcaactgcc aggaacgccg cgcggtcatc gccgcttcct tcagagtaaa
    18061 tctgtccttt gccagcatct gccccatttc gcgatagccc tgcaacactt cattggcgct
    18121 gccttccggg ccatacagca ttggaagcac ctgcttgctg gtgcgatctg gcggcacgct
    18181 gaaggtgttt ccttccgcgt ctaccatatg ttgatcattc caccgcgcaa tcggcacata
    18241 ttcaaccaga tgaatcttca attcatcagg ccactgcttt ctgacgctca cctgcttaat
    18301 ccacggcagg cgttgttcta tttgcgtctg gatgatgttg acatcctggg tcataaaggt
    18361 acccggctca cccaatgcca ggatcgactg ccggatatcg tcattacgcg tgtaatggcg
    18421 ttcaccggtc aacaccagct ttgagagcgg caggcgttgc gcatcttcca tccagcccaa
    18481 cacgacccag ccgctcacca acactgtcgt taaaacggtc agcaggaaaa ggatccccgc
    18541 cagacgcgtt ccattattgc ggcgagaaga aacctcttct tcgctatttc gcgtgttcag
    18601 agcagcctgc gacatattag tccgccagtt ccagaattcg tactaccaac tgcgagaagc
    18661 tcatacctgc ctgacgtgcc gccatcggca ccaggctgtg gctggtcata cccggtgagg
    18721 tattggcttc cagcagataa aactgtccat cgctgtccag cataacgtca atacgtcccc
    18781 atcctttgca acctaacgtc gtccatgctt tcagcactaa tgcctgcaaa ttggcctctt
    18841 gtgacgcttc cagacctgcg gggcagaaat actgtgtctc atcagagaga tacttcgcct
    18901 cataatcata gaaggttccg gacggttgaa tacgtattga cggtaaaatt tcttcaccga
    18961 gtatcgcaac cgtgaactcc ggcccactta gccatttttc aatcaatact tcttcatcgt
    19021 gctgaaatgc caatcttaat gcatcttgta gagcattttc tgctactact tttgacattc
    19081 ccacactgga accttcgcgg ctcggcttaa cgataaccgg caaacccaga gcagaaattt
    19141 ctgctaactg cttatcgctc aggccttttt caaactctgc gcgggttaac gctacccacg
    19201 gcgcgaccgg taaaccggca ccttgccaca gcagtttgct gcgtagttta tccattgaaa
    19261 gcgcagatgc catcactccg ctgccggtat aaggcaagcc catcagctca agcatgccct
    19321 gcagcgtacc atcttcaccg ccgcgaccgt gtagcgcgat aaacactttc tgaaaaccca
    19381 tcgacttcag ttgcgtcacg tcgacttctt tcgggtcgac aggatacgca tcaataccgc
    19441 cttcacgcag cccggctaac accgctgcgc cagaattcag agaaacttcc cgctcagcgg
    19501 aagtcccgcc caacaggacc gcaattttat cagtcatgtt gttcttcctc cggagtttgc
    19561 ggcttcagtt tgatttcagc taaagaacgg gcaatttttc cgatattacc agccccctga
    19621 acgagaatca ggtcgttacc gattaatacc ggtgccagca tctcggctac ctgcgcaggg
    19681 tccggcacca gaatgggatc aattttccca cgtccacgaa ttgtgcgaca cagcgaacgg
    19741 ctgtccgctc ccggaattgg cgcttcgcca gccggataca cttccagcat caacagggta
    19801 tcaacctgcg tcagcacatt ggcgaaatca tcatacaggt cgcgcgtacg ggtaaaacgg
    19861 tgcggctgaa acagcattac taggttttta tccggccagc ctgcgcgcgc cgctttaatg
    19921 gtggcgtcaa cttccgtcgg gtggtggccg tagtcatcga ccagcattgc cgtaccgctt
    19981 ttaccattca ctggctccag cgggaattca ccgaggaaat caaaacggcg accagtcccc
    20041 tggaagctct ccagcgcacg caaaatagcc tcgtcgtcaa ttccctcttc cgtagcaacc
    20101 gcaaccgcag ctgcggcgtt cagcgcgtta tgacggcctg gcgcattcag ggtgacgcgc
    20161 atcggctctt tgtcctggcg cagcagcgta aagtgcccct gcgggccaat ctgctgataa
    20221 tcttctacac gcacgtcggc atcttcgctg aagccgtaag tcgtggtctg acgtcccaca
    20281 cgcggtaaca attcgcggat caccggatca tcaacacaca tcaccgcacg accgtaaaac
    20341 ggcaggttgt gcagaaaatt aataaaagtc tgttttaaat tctcaaagtc gccctggtag
    20401 gtatccatgt ggtcggcttc gatattggtg acaatcgcca ccatcggttg cagatgcagg
    20461 aacgatgcat cactctcatc tgcttcggca atcaggtacc gaccatgccc caaacgcgca
    20521 tgaacccccg ccgcttttac cagcccgccg ttaacgaagg ttgggtcgag ccccgcttct
    20581 gcgtagatgc tggaaaccat cgcggtggtt gtcgttttgc cgtgcgttcc tgcaatggcg
    20641 atgccatgac gaaaacgcat taactcagcc agcatttcgg cacgacggat caccggaata
    20701 cgcgcttcat gagctgcgac aatttccggg ttatcggcag aaatggcgct ggaaacaacg
    20761 accacgctgg catcacgtac gttttccggg cgatggttga aataaatcgt cgctcccaga
    20821 ttcattaact gctgcgtgac cggatttggc gctaaatcgg aaccactgat ctgataacct
    20881 tcattggcca gaacttcggc aataccgccc ataccggcac caccgatgcc gacaaaatgt
    20941 atgtgccgaa cgcgacgcat ttcgggcacg atggaacgca gttttgccaa ttgttgtgta
    21001 ttcattcttt acgccattaa cttcttaaat tcatacgatg caaaaggcat cgctacaatt
    21061 acgcccgggc agcccggctc acttcatttg ccactcgctc ggtggcatcc ggaatggatg
    21121 cagcgcgggc gcgttctgcc atggttaata aggtttctcg cgaccacccg gccagggtgt
    21181 tggcgacagc atccacgcta agctgtggct gctcgataat tttggctgcg cccgcttttt
    21241 ccagcggtag cgcattccag tattgctggc ggtctttatg ttgaaacggc acaaacaacg
    21301 ccggtagtcc tgccgcggcg atttcactca ccgttaaagc accggagcgg caaacgacga
    21361 catccgccca cgcatacgcc gccgccatat catcaataaa ttccgtcact ttatgctgcg
    21421 gttgccccgc ttcggcatac gcctgttcaa cggattgttg cgaaccttta ccgctctgat
    21481 gccagatagt gactgaatca cccagtttcg cagcaacctg cggcattgtc tggttaagaa
    21541 tgcgtgcgcc ctgggaacca cccactacca gcacacgaac cggaccttca cgtccagcca
    21601 aacgttgctg cggcaacggc agcgccagca catcggtacg caccgggtta cccaccactt
    21661 ccgcattagg gaaagcgcct ggaaacgcct gcatcacttt ggtggcaatc ttcgccagcc
    21721 atttattggt taagcccgca ataccgtttt gttcatgaag tacaaccgga atgcctaacg
    21781 accacgcggc cagaccacct ggacctgata catagccgcc cataccgagt accacgtcag
    21841 gtttgtacgc tttcataatc gcccgcgcct gacgccaggc gttgaagata cgcagcggcg
    21901 cagctatcag tgcttttata ccttttccac gcagaccaga gatacgaatg aaatcaattt
    21961 cgatgccatg ttttggcact aagtccgctt ccatacggtc ggcagtcccc agccagcgaa
    22021 cttgccaacc ctgagccatt agatggtgcg caaccgccag tcccgggaat acatgtccac
    22081 cggttccgcc tgccatcacc attaatcgct ttccttgacc actcatcgtg aacctcgtac
    22141 aaacgcctgc gctttctcca gacgcgtttc ataatcaata cgcaacagca tcatgatggc
    22201 tgtcgacata atcagtaagc tcgaaccacc gtaactgatc agcggcaatg tcagaccttt
    22261 ggtcggtaac atccccgccg ccgcgcctac gttaaccagc gcctggaagc taaaccagat
    22321 gccaatagaa caggcgagaa aaccggaaaa acggtggtca atttctaacg ctttgcggcc
    22381 aatcgacatc gcgcgaaaag cgacgaagaa taccattaaa agtgccagca ccacaccgac
    22441 ataccccagt tcttcgccga taatggcgaa aataaagtca gtgtgcgctt ccggcagata
    22501 ctccagtttt tgtaccgagt tacctaaacc ttgtccccaa agttcgccgc gaccaaacgc
    22561 catcagcgat tgcgttaact gatagccgct gccaaaggga tcttcccacg ggttccagaa
    22621 tgcggtaaca cggcggatac ggtacggttc ggcgagtatc agcaacacaa ccgctgaaat
    22681 gcccataccg ataatggcaa tgaactgcca caatttcgct cccgccagga acaacatcgc
    22741 cagcgtagtc acaaacaaca ccaccaccgt accaaggtct ggctgtgcca gcagtaacac
    22801 tgccaacacc agaatcacgc ccatcggttt caggaagccg cgcaggttat tacgtacttc
    22861 gtcgccttta cgcaccagat agttggcgat ataacaaaac agcgacagtt ttgtcagctc
    22921 cgcaggctgg atacgcagca aaccgagatc gatccaacgc gatgcccctt taaccgagct
    22981 acccactacc agcacgatca tcagcaggat gatagatccg agcagcatcg tggcactgta
    23041 gcgttgccag aactccatcg gcagacgcag cgtaatgatc gccagaatga atgccaggat
    23101 cagatagacg ccatcacgct tcgcgaagaa gaacggatcg ttggttaagc gttgccctat
    23161 gggcattgac gccgaggtca ccatgataaa gccaatcgcc gcaaggccga aggtcagcca
    23221 cagtaaggtg cgatcgtaca tgatcaggct gtcggtatct ttttcccgcg agcccatcac
    23281 ccagcctttt agcgccgtgg agatccagac caggatactg aatcctggca ggcgcggcat
    23341 tttcaggcga gggagagata aacgcatcaa cctaactcct tcgccagacg ggcaaactca
    23401 ttgcctcgtt gttcaaagtt cttgaactga tcaaggctgg cacaggccgg ggagagcaga
    23461 accatatcgc ccagctgaac acgcggagcc agcaagcgca tcgcttgttc catggtttcg
    23521 gtttgttctg ccacttccgg gcgtagcgcc gccagctgcg caccgtcacg accaaaacag
    23581 tacaaacgca cgttgtcgcc attcagatag cgcgccagtg gactgaagtc cgccgattta
    23641 ccatcgccgc ccagcaacaa atgcagcgtg ccgtctacct gcaagccatt caacgccgct
    23701 tccgtactgc cgacgttagt ggctttcgaa tcgttaatcc agcgtacgcc attgtgctcc
    23761 agcaccactt caaagcgatg cggcagaccg gtgaaggtgg ttaacgcttt caggctgctg
    23821 gcgcgcggca aaccagctgc gtctgccagt gccagtgctg ccagcgcatt ggtgtagtta
    23881 tgctgcccgg aaagtttcat ctctttcaca ttcagcactt tctcgccttt aacccgcagc
    23941 caggtttcgc cctgctgatg attcaggtga tagtcaccca tgttgacgcc aaagctgacg
    24001 cagcgttcat cagcaccgcg aatcggcatt gttaaggcat catcagcatt gacgacacaa
    24061 actttcgcat tttcgtaaat acgcaatttt gccgcacggt attgctgcaa accaaacgga
    24121 tagcggtcca tatgatcttc agtcacgttc agaatggtcg ccgccaccgc ctgtaagctg
    24181 gaagtggttt ccagctggaa gctcgacagt tccagcacgt acagttcgca ctcagcatca
    24241 agcagcatca acgctggcag gccaatattg ccacccacac caacgttaac ccccgccgct
    24301 ttcgccattt cacccactag cgtggtgacc gtgcttttgc cgttagaacc ggtaatcgcc
    24361 acaatcggtg cttgtgcttc gcgacagaac agctcgatat cgccaacgat ttcgattccg
    24421 gcatcagcgg cagcgcttaa ggatggatgc gcctgtgcaa taccgggact ggcgacaatc
    24481 agatctgccg ccatcagcca ttcatcattc agactgcccg tgtggcgttc tacggcttcg
    24541 ggtaatttat ccaggccagg cggtgtcata cgcgtatcca taacgcgcgg cgtcacaccg
    24601 cgagcgagga aaaagtccac gcaggaaagc ccggtgaggc ccaggccgat aatgacgaca
    24661 tttttaccct gataatcagc catgattaac gtaccttcag cgttgccaga ccaatcagaa
    24721 ccagcatcag cgaaataatc cagaaacgca caatgacgcg cggttccggc cagcctttca
    24781 gttcatagtg gtgatgaatc ggtgccatgc ggaaaatacg ttgtccgcgc agtttaaagg
    24841 agccgacctg caggatgaca gaaagcgttt ctaccacgaa cacgcccccc ataatcacca
    24901 gcaggaattc ctgacgtagc agtacggcga taatgcctaa cgcaccacct aacgccagcg
    24961 aacctacatc gcccataaag acctgcgccg gataggtgtt aaaccacagg aagcccagtc
    25021 ctgccccgac tatcgccgtg cagacaatga ccagttcccc tgcgtgtcgc agatacggta
    25081 tatgcaagta gctggcaaag ttcatattgc cggtcgccca cgccaccagc gcaaaaccac
    25141 cggcgacaaa tacggtcggc ataattgcca ggccgtcgag accatcggtc aggtttaccg
    25201 cgttgccagt acccacaatg acgaagtaag ccagcagaat gtagaacagc cccagctgcg
    25261 gcatcacatc tttaaagaat gggaccacca gctgcgttgc gggcgtgtct ttgccggcaa
    25321 ggtacagggc gaaggcgaca cccagcgcaa tgaccgacat ccagaaatac ttccaacgag
    25381 cgatcaaccc tttggtgtct ttacgcacca ctttgcgata atcatcaaca aagccaataa
    25441 caccgtaacc taccagcacc accaacacgc accagacgta cggattggac gggtaagccc
    25501 acagcagtac ggagatcaca atcgccgtca ggatcataat cccgcccatg gtcggcgtac
    25561 cgcgcttgct gaagtgtgat tcaggaccgt cgttacgcac cacctgacca aaggaaagtt
    25621 tttgcaaatg agctatcata cgcgggccca tccacaatga gatgaacagc gcggtcagca
    25681 ggctgacgat ggcgcgaaac gtcagatagg aaaagacgtt aaagccggaa taatatttga
    25741 ccaaatgttc ggccagccaa actaacatgt cccattctcc tgtaaagcgc gtactacctc
    25801 ttccatggcg gcactacgtg aacccttaac taaaatcgta attacctgtt gctcagcaat
    25861 cagtgattta agacgcgtaa ttaacgcagt tttatcagca aaatgttcgc caacgccgct
    25921 ggcggtgctg atagcatggc tttgtttacc cacgcttaac acgcggtcaa taccagcagc
    25981 ttttgccgcc tcgcccacct gtacatggca ggcttcgctt tcagcgccca gttccgccat
    26041 atcgcccacc accagcacgc ggtagcccgg catttcagcc agtacctgaa cagcggcggt
    26101 cattgaaccg acattggcgt tgtaggagtc gtcgagcagc aactggtttt ctgccagttg
    26161 gatggggaac agacggcctg gaacagcttt cagatttgcc agccccgctt tgatagcatc
    26221 aagcgttgcg cccacggaca tggagagcgc agcggctgcc agcgcattcg caatattgtg
    26281 acgccccggc aacggcagca gaacatcgac gctaccggtt ggggtttgta gggtaaattc
    26341 cgtaccgtgc gaggtcacat ggatattggt ggcggtgaaa tcgctgttgg cggcattggg
    26401 tgagaaacgc cacactttgc gtgagccaat tacgctctgc cagttcagcc agtcgttgtt
    26461 gtcggcgttc ataatggcga taccgttttc cggcaggccg ctaaagattt cacctttcgc
    26521 tttcgcgaca cccgcaagcg agccaaaacc ttccagatgc gccgctgcca ggttgttgac
    26581 cagcgcagct tccgggcgag tcagactcac agtccaggct atttcgccct gatggttcgc
    26641 gccaagttca ataactgcgt aatcgtattc cggcgtcaag cgcaacaacg tcatcggtac
    26701 accgatgtcg ttgttgagat tgcctgccgt ataaagcgtg ttgccgcact ggcttaaaat
    26761 cgccgccgtc atctctttaa cggaggtttt gccggaggaa cctgtcagag caaccacgcg
    26821 cgccggaact tgctggcgaa cccatgcagc cagttcacca aacgccagac gcgtatcctt
    26881 gacgattaac tgcggcaggt cgatatccag cggacggctg accagtagtg cgcccgcgcc
    26941 gccagctttc gcctggtcgg caaaatcatg agcatcgaaa cgttcgcctt tcagggcaac
    27001 aaacaggcag cccggcgtca gttttcgggt atcagtggtt acagcatcaa gggtgatatc
    27061 tgcaccttgc agttcaccgt tgagaatgtc ggtaagttgg ctaagggtta cgctaatcat
    27121 gcaatcaccc ccagcagacg cgccaccgtg acgcgatcgg agtagtccag acgctgattg
    27181 ccaacaatct ggtaatcttc atggcctttg cccgcgacca gtaccacatc attctcttta
    27241 gcctgcataa cggcgcaagt caccgcttca gcacggcctt ccatcacttt ggcatgtccg
    27301 gcatctaaca ttcccgccag aatatcgttg atgatggcac gcggttcttc ggtacgcggg
    27361 ttatcgtccg tcaccaccgc cacgtcagca aactcttcgg caattgcgcc catcagtgga
    27421 cgcttacctt tatcgcgatc gccaccacag ccaaagacgc accacagctt gcccgcacag
    27481 tgcaggcgcg ccgcctgtaa ggctttttcc agtgcatccg gcgtatgcgc gtaatccacc
    27541 accaccgtcg gtttgcctgg cgcagtgaac acttccatac gtccgcaaac cggttgcaga
    27601 cgcgcggcgg ttttcagcag atcagccagt ggatagccga gtgccagcag tgtcgccagc
    27661 gcgagcagca ggttgctgac gttaaaagcg cccatcagat ggctttcaat ttcgccatcg
    27721 ccccaacttg agctaaagcg aatcgtcgca ccgctgtcgt gatagttcac gtcgatcgct
    27781 tttaaccagc gtccgtgaca gttcggatta atatgatctt ccattgatac cgcaaccgcg
    27841 tccggcagtt ttgccagcca gcggcggccc acttcatcgt cggcgttaat aatcgcctga
    27901 ccgcaatgat gttcagaata aagcagccat ttcgcagctt cgtagtgttc catatcacca
    27961 tgataatcaa ggtgatcgcg gcttaagttg gtaaagaccg acgccgcaaa tttcaatgcc
    28021 gccacacggt gctgtaccag cccgtgggag gaaacttcca ttgcgcaaaa cgtcgcgccc
    28081 tgctccacca gccccgccag ctcatgctga acatcgactg cggaaccggt agtattttct
    28141 gtcgggatca ctttcccaag cagaccgtta ccaacggtgc ccattaccgc gctggtttcg
    28201 ccaagcagtt ggctccactg cgccagcagc tgggtagtcg tggttttgcc gttggtgccc
    28261 gttacgccta cgagacgtaa attgtcagag ggttcatggt aaaagcggcc cgccagtgca
    28321 gataaacgct cattgagctg gctgagatag atgaccggta cgccgtgcat ttcacggatt
    28381 tcaccgtcgg tcgcctcatc tttcgctttc gcctctgcaa taatggcagc cacaccttgc
    28441 gctatcgcct gcgggatata tcgacgcccg tccgcctgat gacctactac agctacaaag
    28501 agatcgcccg ccgcagccac acggctgtcg agtgtcatct ctcgcagtgc tcgcgaaggt
    28561 gcgtctggca cccacggagc aagaaggtcg cgcaaattac gatctgccac ctgtcccctc
    28621 gccttgatta atcacaaatt catttttatc gcccgttgtc agcgcatccg gctcgatgtt
    28681 catggtacgc aatacgccgc ccatgatggc accaaagacc ggcgcggaaa cggcgccgcc
    28741 gtagtattta cccgcctgcg gatcgttgat aacaacaacc agcgcgaagc gcggctgact
    28801 cgcaggcgca acgcctgcgg tataagcaat atatttattg atgtagcgac cgtccggccc
    28861 gacctttttc gcggtaccgg ttttaatggc gatacgatag cctttaatcg ccgccttcac
    28921 gccgccgccg cctggtagcg ccacgctttc catcatatgc accacagtgc ggacaatgga
    28981 tcccgggaag acacgttcac cgggaaccgg ggggtcaact ttggtaatcg acagtgggcg
    29041 ataaatgccg tagctgccga tagttgcgta gactcgcgct aactgtaacg gtgttaccat
    29101 tagcccgtag ccgaaagaga aggtggccct ctctatgtca gaccaccgtt gtttttgagg
    29161 atataagcca ctgcgttctc cgaccaaccc caaattggtc gcttttccca gtccaaaacg
    29221 tgagtaagta tctactaacg ctgaggacgg catcgctaac gccagcttgg aaacaccgac
    29281 gttactcgac ttctgtaata ccccggtcag ggttaattcg ctgtagcgtg ccacgtcttt
    29341 gatttcgtgg ccgttaattc gataaggaat ggtattgagt accgagtttt cccgcaccac
    29401 gccacgttgc aacgcggtca ttaccaccat cggtttaacc gttgagcccg gttcaaacac
    29461 gtcggtgatg gtacggttac gcatcgcctc tttcggcgtg ccgctcagat tgttagggtt
    29521 gtatgacggg ctgttagcca tcgccagcac ttcaccggtg ttgacatcca ccagcacggc
    29581 gctaccagat tcagccttgt taaaggccac cgcgttgttc agttcgcgat aaaccagcgc
    29641 ctgcaggcgt tcatcaatac tcagcgccag gttgtgcgct gcctggctgt cagtagaaga
    29701 aatatcttca attacgcgac catagcggtc tttacgcaca atgcgctcac ccggctgccc
    29761 ggtaagccat ttatcgaaac tcttctcaac gccctcaatc ccttgactat cgacgttagt
    29821 aaagccgatg aggtgagcag tcacttcgcc ggacggatag taacggcgag actcttcacg
    29881 cagatgaatc cccggcagtt tcagtttttt gatgtagtcc gccatgtcag ggttcacctg
    29941 acgcgccaga taaataaaac gccctttcgg gttggcgtta atgcgggctg aaagctgatc
    30001 cagcggaata ttgagcgcgt tagccagcgc cttccagcgg tcaccgacgc tgataccgcc
    30061 agcgtcatgc acttctttcg ggtcagccca aatcgctttt accggcacgc tcaccgctaa
    30121 cgggcgacca gaacggtcag taatcatgcc gcgggaggtg gaaacttgct gaacgcgaag
    30181 agaacgcatg tcgccctctt tcaccagcat atccggggag ataacttgta accacgctac
    30241 gcgtccgagc agaaaagcca gcgccaggag aatacagccg cataacaacg caaaacgcca
    30301 actgataaag ttggcatgtt cttcctgacg ttttggtttc tgcgttttcg ccgctgcttt
    30361 catgcgtcgc gtttatcctt atttttgcac tacgatattt tcttgtgacg gatcaacatg
    30421 ctgcatttgc agcttttccg tggcgatcct ttccacccgg ctatggtcgc cgagcgcatt
    30481 ctcttcaagg atcaggttgc gccattcaat gtctaaagca tctcgctcca gcaccagttg
    30541 ttcgcgctga gcggtcagta aacgggtatg gtgcgccgtg gttaccacag tcaccgccgt
    30601 caaaataatg caaatgaaca ggcagagtgg cagcttccca aatcgcaaaa gatcgtcacc
    30661 gataacacca ggcaatgcat ggcgctcgtg gcttcccatc gatcctttaa ctttgcttag
    30721 agcttctgtc actctgctga tcatgcattc gtcctctctg caatacgcag aactgaacta
    30781 cgggcacgag ggttctcagc cacctcttct tcgcccggca ttaacttgcc tagtgctcgc
    30841 agctgacggc cacccagttt tttgagctgc tcttcagtca tcggtaaccc tgccggaact
    30901 tgcggaccgc ggctgttttc acgcataaaa cgtttcacaa tacggtcttc cagcgagtgg
    30961 aagctgatga tcgaaagccg cccacccggg gccagcacgt tgagcgagct ttttagcgcc
    31021 tgctctatct cctccagttc actgtttacc caaatgcgca ccgcctggaa ggtacgggtc
    31081 gcgggatgtt taaacttatc tttcaccggc gttgcaacag ccacgacttc cgccagttct
    31141 ttggtgcggg tcatcggctg ttcgcggtta cgctcgacaa tggcgcgggc aatgcgtttg
    31201 gcaaaacgct cttcaccata ggttttcaat acccaagcga tatcggcttc ttctgcggtt
    31261 tgtagccatt cagcggctga ctgcccacgg gttgggtcca tacgcatgtc cagcggacca
    31321 tcgcgcataa aggaaaagcc gcgctcagca tcatcaagtt gcggtgaaga gacgccaaga
    31381 tcgaggagaa tgccgtcgat cttgccgata agatcgcgct cggcaacgta ttcgcccagc
    31441 gcggagaaag gtccgtggat gatggagaag cgcggatcat caatagtctt cgcaacggcg
    31501 atagcctgcg ggtcgcgatc gatcgccagc aaacgcccct cttcgccaag ctgcgagagg
    31561 atcagacgtg agtgaccacc gcgaccaaaa gtcccatcaa tgtagatgcc atcaggacgg
    31621 atattgaggc cattaacggc ttcatccagc agcaccgtag tatgtttata gttttccatc
    31681 attttataga gacaagtcct gcagtcgctc cgataagtct ccggtagcca actgctctgc
    31741 gtcgatatct tccttgacct gttgatgcca ggttgtttca tcccacagct caaacttgtt
    31801 gaactgtcca accagcatca cttcttttgt cagcccggca tgttgccgca gtactggcgc
    31861 gattaacaat cgacctgcgc catccatctg acattcgctg gcatgaccta acagtaggcg
    31921 ctgcacacgg cgctcaaccg ggttcatgct cgacagacgc gataattttt gctcgataat
    31981 ttcccattca ggcagggggt aaagcagcag gcacgggtga tgaatgtcaa tggtgcaaac
    32041 catttgaccg acagcgttct caagcagctg ttcccggtaa cgggtaggca ctgataagcg
    32101 ccctttgctg tcgagattga ctaacgttgc tccccggaac atgccagcct caccccttat
    32161 tcccactttg ccccacaatt tcccactgaa aggagtttac ggagcggagg aaaagcttgt
    32221 caagccagtc acaaggcata ccgaggtaaa aaacccgatg tttacggcta atatcagcgt
    32281 tgtttatttt acgtccaacg tgcgcagcaa attaacgtta tgaatagctg taagaaaaaa
    32341 tccctcatcg tcatcaccgt tttaaaatga ctcactattt tccacagcaa atataaagtg
    32401 tcagtttgcg acgcgagcgg cattttagga catatcttcc ccggttaaca gtccctgttg
    32461 cgtctgtgtg gcgcttgatg ccagcgggtt tgcgcaaaag caaagaaaag tgcttgttaa
    32521 atcggcaatt catctctgca tgtaacaaaa taatgcaaaa acgcgctgtc gatatcactt
    32581 aattattgtg aatttcagct ggtgagttaa ttaacgggcg ggtttaaaag aacttatcaa
    32641 taatagtcag gaatagtctt atttacttta aggatattga tgtccattcc cgtactctac
    32701 g
//
LOCUS       DAZPAC010000022        32532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZPAC010000022 DAZPAC010000000
VERSION     DAZPAC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.022"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..93
                     /locus_tag="ACHUPM_000860"
     CDS             <1..93
                     /locus_tag="ACHUPM_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0868722.1"
                     /translation="SLLAKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(135..1211)
                     /gene="glpQ"
                     /locus_tag="ACHUPM_000861"
     CDS             complement(135..1211)
                     /gene="glpQ"
                     /locus_tag="ACHUPM_000861"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0868723.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1216..2574)
                     /gene="glpT"
                     /locus_tag="ACHUPM_000862"
     CDS             complement(1216..2574)
                     /gene="glpT"
                     /locus_tag="ACHUPM_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR0868724.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2847..4475
                     /gene="glpA"
                     /locus_tag="ACHUPM_000863"
     CDS             2847..4475
                     /gene="glpA"
                     /locus_tag="ACHUPM_000863"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR0868725.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4465..5724
                     /gene="glpB"
                     /locus_tag="ACHUPM_000864"
     CDS             4465..5724
                     /gene="glpB"
                     /locus_tag="ACHUPM_000864"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR0868726.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5721..6911
                     /gene="glpC"
                     /locus_tag="ACHUPM_000865"
     CDS             5721..6911
                     /gene="glpC"
                     /locus_tag="ACHUPM_000865"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR0868727.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7104..8006
                     /locus_tag="ACHUPM_000866"
     CDS             7104..8006
                     /locus_tag="ACHUPM_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR0868728.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8047..8850)
                     /gene="yfaU"
                     /locus_tag="ACHUPM_000867"
     CDS             complement(8047..8850)
                     /gene="yfaU"
                     /locus_tag="ACHUPM_000867"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFR0868729.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8868..10157)
                     /locus_tag="ACHUPM_000868"
     CDS             complement(8868..10157)
                     /locus_tag="ACHUPM_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0868730.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10214..11418)
                     /gene="rhmD"
                     /locus_tag="ACHUPM_000869"
                     /pseudo
     CDS             complement(10214..11418)
                     /gene="rhmD"
                     /locus_tag="ACHUPM_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(11433..12215)
                     /locus_tag="ACHUPM_000870"
     CDS             complement(11433..12215)
                     /locus_tag="ACHUPM_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFR0868731.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12435..13637)
                     /locus_tag="ACHUPM_000871"
     CDS             complement(12435..13637)
                     /locus_tag="ACHUPM_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR0868732.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(13737..14279)
                     /locus_tag="ACHUPM_000872"
     CDS             complement(13737..14279)
                     /locus_tag="ACHUPM_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR0868733.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14558..14983
                     /gene="nudI"
                     /locus_tag="ACHUPM_000873"
     CDS             14558..14983
                     /gene="nudI"
                     /locus_tag="ACHUPM_000873"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR0868734.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15022..15624)
                     /gene="ais"
                     /locus_tag="ACHUPM_000874"
     CDS             complement(15022..15624)
                     /gene="ais"
                     /locus_tag="ACHUPM_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR0868735.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            15932..17071
                     /gene="arnB"
                     /locus_tag="ACHUPM_000875"
     CDS             15932..17071
                     /gene="arnB"
                     /locus_tag="ACHUPM_000875"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR0868736.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17075..18043
                     /gene="arnC"
                     /locus_tag="ACHUPM_000876"
     CDS             17075..18043
                     /gene="arnC"
                     /locus_tag="ACHUPM_000876"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR0868737.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18043..20025
                     /gene="arnA"
                     /locus_tag="ACHUPM_000877"
     CDS             18043..20025
                     /gene="arnA"
                     /locus_tag="ACHUPM_000877"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFR0868738.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20022..20912
                     /gene="arnD"
                     /locus_tag="ACHUPM_000878"
     CDS             20022..20912
                     /gene="arnD"
                     /locus_tag="ACHUPM_000878"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR0868739.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            20912..22564
                     /gene="arnT"
                     /locus_tag="ACHUPM_000879"
     CDS             20912..22564
                     /gene="arnT"
                     /locus_tag="ACHUPM_000879"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR0868740.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22561..22896
                     /gene="arnE"
                     /locus_tag="ACHUPM_000880"
     CDS             22561..22896
                     /gene="arnE"
                     /locus_tag="ACHUPM_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR0868741.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            22896..23282
                     /gene="arnF"
                     /locus_tag="ACHUPM_000881"
     CDS             22896..23282
                     /gene="arnF"
                     /locus_tag="ACHUPM_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR0868742.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23276..23542)
                     /gene="pmrD"
                     /locus_tag="ACHUPM_000882"
     CDS             complement(23276..23542)
                     /gene="pmrD"
                     /locus_tag="ACHUPM_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR0868743.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(23586..25007)
                     /gene="menE"
                     /locus_tag="ACHUPM_000883"
     CDS             complement(23586..25007)
                     /gene="menE"
                     /locus_tag="ACHUPM_000883"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR0868744.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(25004..25966)
                     /gene="menC"
                     /locus_tag="ACHUPM_000884"
     CDS             complement(25004..25966)
                     /gene="menC"
                     /locus_tag="ACHUPM_000884"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR0868745.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAEMADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(25966..26823)
                     /gene="menB"
                     /locus_tag="ACHUPM_000885"
     CDS             complement(25966..26823)
                     /gene="menB"
                     /locus_tag="ACHUPM_000885"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR0868746.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(26838..27596)
                     /gene="menH"
                     /locus_tag="ACHUPM_000886"
     CDS             complement(26838..27596)
                     /gene="menH"
                     /locus_tag="ACHUPM_000886"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR0868747.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27593..29263)
                     /gene="menD"
                     /locus_tag="ACHUPM_000887"
     CDS             complement(27593..29263)
                     /gene="menD"
                     /locus_tag="ACHUPM_000887"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR0868748.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29352..30647)
                     /gene="menF"
                     /locus_tag="ACHUPM_000888"
     CDS             complement(29352..30647)
                     /gene="menF"
                     /locus_tag="ACHUPM_000888"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR0868749.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30726..31031)
                     /gene="elaB"
                     /locus_tag="ACHUPM_000889"
     CDS             complement(30726..31031)
                     /gene="elaB"
                     /locus_tag="ACHUPM_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR0868750.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31086..31529)
                     /locus_tag="ACHUPM_000890"
     CDS             complement(31086..31529)
                     /locus_tag="ACHUPM_000890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0868751.1"
                     /translation="MHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDLTGDNRH
                     ILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDTCTHHWP
                     DKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            31612..32529
                     /gene="rbn"
                     /locus_tag="ACHUPM_000891"
     CDS             31612..32529
                     /gene="rbn"
                     /locus_tag="ACHUPM_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR0868752.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         7896 a         8806 c         8138 g         7692 t
ORIGIN      
        1 tcgttgttgg ctaaagaaaa gccggtagaa ggcagtccac tgtttttact acaaatgtgc
       61 atggaacaat tagtggcgat tggaaaaatt tgatatgaga caggatggcg catgaacgcc
      121 atcctgcagt agcattactc tttattaagg aattttactg ccttatcagg gaaatcagta
      181 aacagcccat ttacacccgc tttgttatac agagcatcat ataactgatt cacatcagta
      241 gtgtattcag gcagtttatc cgaccgcacg gtataaggat gcactaccag tttattctgc
      301 tgagcatctt gcaccatgcc agtgagtttg atattacccg gctgcgatgt ttcctcaatc
      361 aacatatggt aatccggccc gataccgtcg gcatattccg cgacttgctt catggcacct
      421 ggcttaaaca tccagtcata gctgtaatta acccagcttc cgtccggctg tttctgctgc
      481 gtttcattcc agtcggtata ggcaatcagc tgtaccagat tgagatccat gcccatttta
      541 ggttccagct cattcttaat acgcttcagc tcatcagcat caaaacattg caaataaact
      601 ttatcgtcct taccggtgta accatatttc ttcagcactt ccagcgtttt tgccgcaata
      661 tccttccctt cctgatgatg gaaccacggc gctttgattt ctggatagat accgatattt
      721 ttcccggtag agtgatttaa cccctgaaca aattcaatct cttcttcaaa ggtgtgcccc
      781 cggaagtcgg acttacccat tgggaaacgc cccggataag tctgtacttt tttaccgttt
      841 tcaatatcga aaccttcggt aaatttcagc gacttaattt catccagcgt gaaatctatc
      901 gcgtagtaac gaccgtcttt gcgcgcccga tccgggaaac gatcggcaac atcagtaaca
      961 cgatcgagat aatggtcatg cagaacaacc agatggtcgt ctttggtcat caccaaatcc
     1021 tgttccagat aatccgctcc ctgcgcatac gccatcgctt ttgctggcag cgtatgctcc
     1081 ggcaaatatc cactggcacc gcgatgggcg atgactattt tttcgttgct gtccgccgcc
     1141 attgcactgc ttcccatgac tatagtgctc atcatgatcg ccatgctaag gtttttcagc
     1201 ttcaatttca tgccattagc ctccgttgcg tttttgcagt aattgttcat ggcgacgttt
     1261 ttcgccaatc atcacaacaa tcaacaagat aaccgccaga atgctgccgc caatcattac
     1321 cataaagccg ccatcccagc cgaagaagtc cacggtgtag ccaacaatcg cgctcgccgc
     1381 caccgaaccg cccaggtaac caaacagccc ggtaaagccc gctgccgtac ctgccgcttt
     1441 tttcggtgcc agttccagcg catgcagacc gatcagcatc acaggaccgt agatcaggaa
     1501 gccgataaca atcatacaaa tcatatcgac ggttgggtta cctgccgggt tcatccagta
     1561 aacgatagtc gcgatggtca ccagtgtcat aaagaaaacg ccggttgccc cacggttgcc
     1621 acggaagact ttatccgaca tccagccgca cagcagagtg cccggaatac ctgcatattc
     1681 ataaaggaag taggcccagg aggatttatc cagcgcgaaa tgcttaacct ctttcagata
     1741 agtcggtgac cagtcgagga tgccgtaacg cagcagataa acgaacacgt tggcgatggc
     1801 gatataccac agcagtttgt tcggcagtac gtactgcatg aagatttgct tcgccgtcag
     1861 ctcctgttcc gctttttcgt tatagtcgtc cggataatca tttttgtact cttcgatcgg
     1921 cggcaagcca caggattgcg gggtatcgcg catcatcgca aaggcgaata atgccaccag
     1981 aatggcgcag aaagcaggca tatagagcgc cgcatgccag tcattgaacc aggccatccc
     2041 cagcaggaac agcagcggcg gaataccacc accgacgttg tgcgcacagt tccacactga
     2101 cacaatgccg ccacgttctt tctgcgacca ccagtgcacc atagtacgac cacacggcgg
     2161 ccaccccatc ccctggaacc aaccgcagag gaacaacagt acaaacatca ccgcaatgct
     2221 cgacgtcgcc catggcacaa agcccataaa caacatcact gccgccgcca gaatcaaacc
     2281 tgcgggcagg aaaacgcgcg gattcgagcg atccgatacc gaacccatga tgaatttcga
     2341 aaatccataa gcaatcgaga tccccgaaag ggcaaaacct aaatcaccgc gtgagaatcc
     2401 ctgctcaacc agataaggca tagcaagcgc aaagttctta cgaaccaaat agtaagccgc
     2461 atagccaaag aatatcccca ggaaaatttg ccagcgcaat cgacgataag tcggatcgat
     2521 ctccgcggca ggtaagcgcg ctttgtgtgg cgctggttta aaaatactca acattgatag
     2581 cctccgtggc ccgtggtctt atttatgatt aacagcctga ttcagtgaga gaacctgccg
     2641 tttcttgagt tgccgcgatg ttaagaaaac attcataaat taaatgtgaa ttgccgcaca
     2701 cattattaaa taagatttac aaaatgttca aaatgacgca tgaaatcacg cttcactttc
     2761 gaatcatgag cgaatatgcg cgaaatcaaa caattcatgt ttttactatg gctaaatggt
     2821 aaaaaacgaa cttcagaggg ataacaatga aaactcgcga ctcgcaatca agtgacgtga
     2881 ttatcattgg cggcggcgca acgggagccg ggattgcccg cgactgtgcc ctgcgcgggc
     2941 tgcgcgtgat tttggttgag cgccacgaca tcgcaaccgg tgccaccggg cgtaaccacg
     3001 gcctgctaca cagcggtgcg cgctatgcgg taaccgatgc ggaatcggcc cgcgaatgca
     3061 ttagtgaaaa ccagatcctg aaacgcattg cacgtcactg cgttgaacca accaacggcc
     3121 tgtttattac cctgccggaa gatgacctct ccttccaggc cacttttatt cgcgcctgcg
     3181 aagaagcagg gatcagcgca gaagctatag acccgcagca agcgcgcatt atcgaacctg
     3241 ccgttaaccc ggcattgatt ggcgcggtga aagttccgga tggcaccgtt gatccatttc
     3301 gtctgaccgc agcaaacatg ctggatgcca aagaacacgg tgccgttatc cttaccgctc
     3361 atgaagtcac gggactgatt cgtgaaggcg cgacggtgtg cggtgttcgt gtacgtaacc
     3421 atctcaccgg cgaaactcag gcccttcatg cgcctgtcgt ggttaatgcc gccgggatct
     3481 gggggcaaca cattgccgaa tatgccgatc tgcgcattcg catgttcccg gcgaaaggat
     3541 cgctgctgat catggatcac cgcattaacc agcatgtgat caaccgctgc cgtaaacctt
     3601 ccgacgccga tattctggtg cctggcgata ccatttcgct gattggcacc acctctttac
     3661 gtattgatta caacgagatt gacgataatc gagtgacggc agaagaggtt gatattctgc
     3721 tgcgtgaagg ggaaaaactg gcccccgtga tggcgaaaac gcgcattttg cgggcctatt
     3781 ctggcgtgcg cccgctggtt gccagcgatg acgacccgag cggacgtaac gtcagccgtg
     3841 gcatcgtgct gctcgaccat gctgaacgcg atggtctgga cggatttatc accatcaccg
     3901 gtggcaaact gatgacctat cggctgatgg ctgaatgggc taccgacgcc gtgtgccgca
     3961 aactgggcaa cacgcgcccc tgtacgaccg ccgatctggc acttcctggt tcacaggaac
     4021 ccgctgaagt taccttgcgt aaagtcatct ccctgcctgc cccgctgcgc ggttctgcgg
     4081 tttatcgtca tggcgatcgc acgcctgcct ggctgagcga aggtcgtctg caccgtagcc
     4141 tggtatgtga gtgcgaagcg gtaacagcgg gtgaagtgca gtacgcggta gaaaatttaa
     4201 acgttaatag cctgctggat ttacgccgtc gtacccgtgt ggggatgggt acctgccagg
     4261 gcgaactctg cgcctgccgc gctgccggac tgctgcaacg ttttaacgtc acgacgtccg
     4321 cgcaatctat cgagcaactt tccaccttcc ttaacgaacg ctggaaaggc gtgcaaccca
     4381 tcgcctgggg agatgcactg cgcgaaagcg aatttacccg ctgggtttat cagggactgt
     4441 gtggtctgga gaaggagcag aaagatgcgc tttgatactg tcattatggg cggcggcctc
     4501 gccggattac tctgtggcct gcaactgcaa aaacacggcc tgcgctgtgc cattgtcacg
     4561 cgtggtcaaa gcgcgctgca tttctcatcc ggttcgctgg atttgctgag ccatctgcct
     4621 gatggtcaac cggtgacaga cattcacagt ggactggaat ctttgcgtca gcaggcacca
     4681 gcccatcctt acagtcttct cgaaccacaa cgcgtgctcg atctcgcttg ccaggcgcag
     4741 gcattgatcg ctgaaagcgg tgcgcaattg cagggcagcg tggaactggc ccatcagcgc
     4801 gttaccccgc tcggcacttt gcgtgcaacc tggttaagtt cgccagaagt tcccgtctgg
     4861 ccgctacccg cgaagaagat atgtgtagtg gggataagcg ggctgatgga tttccaggcg
     4921 catcttgcgg cggcttcgtt gcgcgaactg gatctttcgg ttgaaaccgc agaaatagag
     4981 ctgccggaac tggatgtcct gcgcaataac gccaccgaat ttcgcgcggt gaatatcgcc
     5041 cgtttccttg ataatgaaga aaactggccg ctgttacttg atgcgcttat tcctgtcgcc
     5101 aatacctgcg aaatgatcct gatgcccgcc tgcttcggcc tggccgatga caaactgtgg
     5161 cgttggttga atgaaaaact accttgttca ctgatgcttt tgccaacgct gccgccttcc
     5221 gtgctgggca ttcgtctgca aaaccagtta cagcgccagt ttgtgcgcca gggtggcgtg
     5281 tggatgccgg gcgatgaagt gaaaaaagtg acctgtaaaa atggcgtagt gaatgaaatc
     5341 tggacccgca atcacgccga tattccgcta cgtccacgtt tcgcggttct cgccagcggc
     5401 agtttcttta gtggcggact ggtagcggaa cgtaacggca ttcgcgagcc gattctcggc
     5461 cttgatgtgc tacaaaccgc cacgcggggt gaatggtata agggcgattt ttttgcgccg
     5521 caaccgtggc agcagttcgg tgtaaccact gatgagacgc tacgcccgtc gcaggcaggg
     5581 caaaccattg agaacctgtt tgccatcggt tcggtgctgg gcggatttga tcccatcgcc
     5641 caaggatgcg gcggcggtgt ttgtgccgtc agtgctttac atgccgctca acagattgcc
     5701 caacgcgcag gaggccaaca atgaacgaca ccagcttcga aaactgcatt aagtgcaccg
     5761 tctgcaccac cgcctgcccg gtgagccggg tgaatcccgg ttatccaggg ccaaaacaag
     5821 ccgggccgga tggcgagcgt ctgcgtttga aagatggcgc actgtatgac gaggcgctga
     5881 aatattgcat caactgcaaa cgttgtgaag tcgcctgccc gtccgatgtg aagattggcg
     5941 atattatcca gcgcgcgcgg gcaaaatatg acaccacgcg cccgtcgctg cgtaattttg
     6001 tgttgagtca taccgacctg atgggtagcg tttccacgcc gttcgcacca atcgtcaaca
     6061 ccgctacctc gctgaaaccg gtgcggcagc tgcttgatgc ggcgttaaaa atcgatcatc
     6121 gtcgcacgct accgaaatac tccttcggca cgttccgtcg ctggtatcgc agcatagcgg
     6181 ctcagcaagc acaatataaa gaccaggtcg ctttctttca cggctgcttc gttaactaca
     6241 accatccgca gttaggtaaa gatttaatta aagtgctcaa cgcaatgggt atcggtgtac
     6301 aactgctcag caaagaaaaa tgctgcggcg taccgctaat cgccaacggc tttaccgata
     6361 aagcacgcaa acaggcaatt acgaatgtag agtcgatccg cgaagctgtg ggagtaaaag
     6421 gcattccggt gattgccacc tcctcaacct gtacatttgc cctgcgcgac gaatacccgg
     6481 aagtgctgaa tgtcgacaac aaaggcttgc gcgatcatat cgaactggca acccgctggc
     6541 tgtggcgcaa gctggacgaa ggcaaaacgt taccgctgaa accgctgccg ctgaaagtgg
     6601 tttatcacac tccgtgccat atggaaaaaa tgggctggac gctctacacc ctggagctgt
     6661 tgcgtaaaat cccggggctt gagttaacgg tgctggattc ccagtgctgc ggtattgcgg
     6721 gtacttacgg tttcaaaaaa gagaactacc ccacctcaca agccatcggc gcaccactgt
     6781 tccgccagat agaagaaagc ggcgcagatc tggtggtcac cgactgcgaa acctgtaaat
     6841 ggcagattga gatgtccaca agtcttcgct gcgaacatcc gattacgcta ctggcccagg
     6901 cgctggctta aactcctttc tgatgcccgg taagcatgtg gttaccgggc atttttgcgt
     6961 acacgattcc gtgcccaatg tatgcgttgc aacgcagtga aaattcctct gaaaacgtct
     7021 cgcaaaggct gaaactggca gatgtcaaag gcctgggata accgtaatgt cgcgtcatca
     7081 taaatatcag gtgacggaca accatgaccg aatcaacaac ctcctccccg catgatgcgg
     7141 tatttaaaac ctttatgttc acacccgaaa ccgcacggga ttttctcgaa atacatttgc
     7201 cagaaccact gcgcaagctt tgcaacctgc aaaccttacg cctggaaccc actagtttta
     7261 ttgaaaaaag tttacgcgct tactactcgg atgttttgtg gtccgtggaa accagcgacg
     7321 gtgacggcta tatctactgc gtgattgaac atcaaagctc tgcagaaaag aatatggctt
     7381 ttcggctaat gcgctatgcc actgccgcca tgcagcgtca cctggataaa ggctatgaca
     7441 gagttccgct ggtggtgcca ttgctgtttt atcatggcga aacatcgccc tacccgtact
     7501 cacttaactg gctggatgag tttgacgatc cgcaacttgc ccggcagttg tacaccgaag
     7561 cttttccgtt ggtggatatc accatcgtac ctgacgatga gatcatgcaa catcggcgta
     7621 tagctctgct tgaactgatt caaaagcata ttcgcgaccg cgatttaatc ggtatggtcg
     7681 acaggatcac cacgcttttg gttaaaggct tcactaatga cagccagcta caaacactgt
     7741 ttaattatct gctgcaatgc ggcgatacct cccgtttcac ccgttttatt gaggagattt
     7801 ccgaacgttc accactacaa aaggagagat tgatgactat tgctgagcgg ctacggcagg
     7861 aaggacacca aattggctgg cagaaaggta tgcatgaaca agccattaaa attgctttgc
     7921 gcatgctgga acagggcatt gatcgtgacc aggtgctcgc ggccacccag ctaagcgaag
     7981 ccgatctggc agcgaataac cactaattaa cacaggccca cagccgatcc ccatgggcct
     8041 ttgatatcaa taactacctt ttatgcgtgg cccatttttg cctgatttaa acatcgccag
     8101 tcgttgatcc agggcatcgc tgtagagcat cgtgtcaacg ccaacagcga caaagttcgc
     8161 tccccacgcc aggcattgct gcgccatatc aggagccaca gccagaaaac cagccgcttt
     8221 acccgcagca cggatccgcc gaatgctggt ttcaataatt cgctgcactt ccgggtgccc
     8281 ggcgttatcc gggtagccca acgacgcaga aagatccgct ggtccaataa acacgccatc
     8341 aatcccttcg acgtcgagga tctcgtccag gttatccagt gccgttttac tttccacctg
     8401 cactaacaga caaagcgaat cgttgacttg cgccatgtaa ttctcaatgc gtccccagcg
     8461 cgcagcccgt gccacactgg ccccaacacc acgctcaccg tagggaggat agcgcgtggc
     8521 agacgccacc tgacgagcct gatcggcagt atcgaccatc gggatcaata gagtttgcgc
     8581 gccaatatcc agaacttgtt taatcagcga tttactgcct tccaccggac ggatcacggg
     8641 ttggctggca tagggcgcta ccgcctgtag ctggtgataa agatcctgaa tggtgtttgg
     8701 cgcgtgctcc ccgtcaatca gcaaccagtc ataaccggaa gtagcggcaa tttctgccat
     8761 ataagaagta gtcgagctta accacagacc aatttgcact tcgcccttgc gtaaacgttc
     8821 tttaaaggga ttggttaata atgcgttcat cgtgttcctt aacctattta atgatgtgcc
     8881 acgtcggtct gcgcaacagt acgatttaca cgcagagtga aaataatcag cgaaccgatg
     8941 accgccaccg ctgccagtgt cagtaatcct gccgcatcgc tggcaaataa cgtttctgct
     9001 ttcacgcgca ggatcggggc aataaacccg cccactgcac caaacaggtt tacaaaacca
     9061 atccccgcag ccagcgccgt accggaaagc agttgtgtcg gcatcgtcca gaacaccggc
     9121 tgcacggcaa taaacccgat agctgcaaca cacagcgcta cgatcgccat tactggagaa
     9181 agcagcccgg acagaccaat accaatgccc gccgccagta atgtcagcgc tgcgacatta
     9241 cgccgttcgc cggttttatc ggaatagcgc ggaataagcc aggtcccaaa caaggccgca
     9301 acccacggaa tggcggtgac caccgacgct gtaaagccca cttttgttcc cagcaatgcc
     9361 gcaacctggg tcggcaggaa gaaaattaat ccgtaaaccg ccacctgaat ggtcaggtag
     9421 ataatcgcta gttgccagac tcggccatta cgcagcgcat cgctcagccg agaagtcact
     9481 ttctgttgtt cttcacttgc cagttgattg ataagcaacg ttttttcttg tttactcaga
     9541 aaacgtgcct gctccggtgt gtcatcaagc caaaagaatg tgaatacccc agcaccgact
     9601 gccaacaatc cttcaatcac aaacatccag aaccagccgg gatgccccat aaatccatgc
     9661 atctccaaca gcgcgccgga aagcggtgat cccagtgtta acgccagcgg tgcgcccata
     9721 tagaacagcc ccataatgct ggcgcgatta cgctgcggaa accattgcga ggtgagataa
     9781 atcataccag ggaaaaatcc ggcctccgca gcaccaagca gagtgcgaac tatcagaaat
     9841 ttcgcttcag tatcggccca tgccatggct gccgaaagaa atccccacag cagtgttgtc
     9901 gtaccaatcc aggttctggc ccccagtttg cgcatcaaaa gattcgccgg aacacccaga
     9961 aacgcatata ccacaaagaa aatgcctgct cccagcgcat aagcttcatt actcaacccg
    10021 gtatcaatct ggtaggtctg tttggcaaaa ccgatattcg aacggtcgag aaacgccagc
    10081 acatacagcg ccaacataaa cggaattaaa cgcacacggt ttttcttcac cacggcgtca
    10141 agcaaagcgg tgctcataaa aagctcctta gaatatgtgg gcgttaccgt tgtcacagca
    10201 acgcccggag tgattagtgg ctgtaggggc gtttcaaatt gcagtcacga ttgagttcga
    10261 cgccaaaacc gggtttatca agcactgatt tatgaatacg accattcacc ggaaccggct
    10321 cattgagcag aatcgggtca aactgcggac gcatcgttga acaatccggg ctggtcatca
    10381 ggaattcgct gaatggcgta ttggtgaagg tgatcaccgc atggtgagag taaacagacg
    10441 aaccgtgcgg caccaccagt tgccccctgg atttggcgat tgcggcaatt tccaccagcg
    10501 tggttaaacc accgcaccag ccaacatccg gctgcataat gtcgataccg gtttctgaaa
    10561 gcgtacgaaa agattgcagt gtgccgtggt gctcaccgct ggtgaccatc attccgactg
    10621 gcgcgttgcg tttcagttcg cgataacctt catactgctg tggcggcagg cactcttcga
    10681 tccatttcag gttatagggc gcgcaagcgt gggccagttt ggtggcatag ttcacgtcct
    10741 gactcatcca gcagtcgagc attaaccaga aatcctcacc gcatttttca cgcatatccg
    10801 cgaccataac ggcatctttg cggatccccg catcgccatc atgtggcccc cagtgcgtcg
    10861 gcattttgcc accgataaag cccatctctt ttgccagatc cggacgcgcg cctgtggcgt
    10921 agaactgaat ctcatcacga actgcgccgc ctaaaagttt ataaaccgga agcccgacca
    10981 ctttgccgaa cagatcccac agagccagat cgacacagga aatcgtgttc atcaccaggc
    11041 caccagagcc ggagtaatac agggtggcat tgagcatttg atcatggatc agtttgatat
    11101 cactgacaca tttaccctca atgaaacggt taagatgttt ttcgacaata aaacagccca
    11161 tttcaccggc tgtcgaaaca gcgaatccgg tctggccgtt ttctgcttcg acttcaacaa
    11221 tcaacgtgcc aagaacgtta atgccaaatg actggcgtga ctgctcgtaa tcgcggtatt
    11281 tactcatcgg ggtggcaata tgatcgtcaa tccagtgatt cgccccctgg tcgtgataat
    11341 caccgcgcca gcaccttttt ctgctgtcgc accgccagta aaccaggcgc gaacctgttt
    11401 gatttttggt agggtcatga tgttctccat tgttatgagg cctgtaagtc aaagggactt
    11461 ttccatccca acagacgtga aatatccctg gcgcaggcaa tggccttgcc cgccagataa
    11521 tcacggtatt cttcattgat ttgtaagcgg gtaccgacca ccgagatcgc agcggtaagc
    11581 tcgttattgg cgttaaacac cggcgcagcg acacaacgga catcggggta atcttcgccg
    11641 ttgtcatagc tccagccctg acggcgaata cgcgccagtt cttcgtgaag ttgctgtgga
    11701 tgagtaatcg ttgtgggtgt cgcctgctcc cagaccagcc cttcgataat actttgctgt
    11761 accgctgcag gttgccaggc aagcaggcat ttacctattc cggagcgata aagcgaaagg
    11821 ctttttcctt catgggaacg cacgctgata gtggctgatg actccacttt taaaatgtaa
    11881 taagcgctgc cgttatcaat aatccccagg tgacataaca gcccggtggt atccatcagt
    11941 tgcgtcaaac gcggtcgagc cagttcccgg agatccatct tgcttaacgc atgaccagaa
    12001 agctccacca gcctggtcca caaacaaaaa ttttcctggt gatcaaggct aagaaaacgc
    12061 tgacgcctga gttcattaag cagcaaatag gccgtgcttt tagggattcc caatgtgtca
    12121 atgatcgtcg cagcactaca ggggccaatg cgggcaatca aattgagaat atcaatagcc
    12181 cgtgtcaggg cgggaacttt gcttgattcc aacatactgg actccagtct tagatactgg
    12241 aatcagtctc gttgctcagg gggagtaaaa ttgtgaagcg catcaaatcc cgctcctcgt
    12301 ccagcggact ggagaaatta cgcacaaatt tgtgaatcag tgcacggatt ttttcttatt
    12361 gagtacaaca ggttggacaa gttttgccgg agggggaatg tgccggtgca gtaaaccggc
    12421 acagagtggc ggaatcagac agataaggtc atggactcaa caacctcaat ccagccatgc
    12481 tcactggcga tatcctggcc gtttaaccaa cggcgcagca tattcagtgc catcattgcg
    12541 cacacttcct gacggatagc caggctgtag cgtgtagtgc tgaaacgcac acgcagagcg
    12601 aaagtgccgt ctggcgtggc tagcgcaaag ttgagatgct cgttctcgaa acccgaaaca
    12661 gccagtgcca gcccggcaaa atggttggcc cgccgttctg taatccagtg cgcagtttgc
    12721 gccagggttt cttcctgtga aggaaccact tcacacgcca gcaatggagc acctgcgcga
    12781 gaaagttgca aagccaataa accaccggtg aattgctcgc tcaacgtcag gctgaactgg
    12841 cggttttgca attcgcgact gatctgcgcg ggcagtcctt cggtgccttc gaaaatcacg
    12901 ctctgtccgg caacacgttt aacgtccagc cacagttttt ccatcgcctg ttgctcgctt
    12961 gccggtccgg tgagtttcag ttcgatgatc ggcattgagg agcgatagcc cattgttacg
    13021 cccggcggca gttgtagagt gtccaggctt tgtgccagat cgctttccga acgaccaaag
    13081 gtagttaaac gcagacaaac cggcgactgt ggtaaagaaa aacgctcgcg caggcgcggc
    13141 aggatttcgt gctcgaccat caccttaaat tctgacggta cgccgggagt aaagaacatc
    13201 aggcaacgat taagccgcac ggcaaaacca caggcggtgc ctaccgggtt attgataaat
    13261 tcagcactgg caggcagctc cgcttgttta cggttgctcg gtgccattac tcgtccacgt
    13321 tcgtggaaat agcgttccat ctctttgagc caggcttcat gcagcaccag gccttcacct
    13381 tttgctgtcg cagcggcgag tgcacttaaa tcatcactgg tcggtcccag cccgccgtta
    13441 acgatcagca catcggcgtg ctgactacgt tcgcgaagaa tggtgactaa gtcatcaagg
    13501 ttatccccca ccgtattgcg gcgagataat ggcaacccct gatgaaagaa aaaatcggcc
    13561 agccaggcag cgttagtgtc aacgatttgc ccgtgtaaca cttcatcccc ggtggataac
    13621 atttccactt ttaacatggt tatctcctgc ttttgttatc gaatcactat aacgcaaagg
    13681 gagataaggc gtagtaagaa actgaatggc gcgacagaat gccgcgccgg aagggatcag
    13741 aaactggcgt taaccccaac atacgggccg tcagccacgg cgttgtcgcg gttaccgtct
    13801 ttacccgaca gattcaggta gcgataaccc gcctcaatac tgactggatg cataatggtg
    13861 taacgcgcgc cagcattcgc ttcctcataa cttttaatac cgctggagag tgaatccgga
    13921 gagtagtaat actcgccaaa caaacggaag ctgttgccaa ttttccactg caaaccacct
    13981 cctaccgctg cggcataacc ttcatcgccg taattcgggt tggtgtaaac gccttttccg
    14041 ccaacggtcg ccattaacgg cccgagaggc agattcaacc ccagccccac gccagcgacg
    14101 tcaccgtcgt cgtcgttatg tgtccagtta ccgcttaacg ccaggcccgt cgattcagtc
    14161 ccaaaaccga caccaatatt ggtgtattct ttacccgcct gaccgctgat gctcattgca
    14221 ttgaccgatg cagaaaccaa cagcattccg gccagacctg ctagcgcaat ttttttcatt
    14281 atcccctttc ccacaagcaa ttaatcaaaa ttcccaaagc tgcgggattg tactgcctgg
    14341 cacagaggat tcaatgagcg aaaaaaggga tctagtcact tattgtaact aaatacttct
    14401 taaataatta aacgccgaaa aaataaacac actttaccga tacacacaaa aataataata
    14461 agtgcagtta tcatcgcaaa agagattact gtcactttca catatttctg agttaccgta
    14521 ttctcatggt ttctttttat ttataaggaa tacccctgtg cgacaacgga ctattgtatg
    14581 ccctttgatt caaaatgatg gtgcttattt gctgtgcaaa atggccgacg atcgcggcgt
    14641 tttccccggt caatgggcgc tttcgggtgg cggcgtggag tctggcgaac gaattgaaga
    14701 ggcactacgc cgcgaaattc gcgaagaact gggagaacag ctgcttttga cagaaatcac
    14761 gccgtggacc ttcagcgatg atattcgcac caagacgtat gcagatggtc gcaaggaaga
    14821 gatttatatg atttacctga tttttgactg cgtttctgcc aaccgagaag tgaaaataaa
    14881 cgaagagttt caggactacg cgtgggtaaa acctgaagat ctggtgcatt atgatttaaa
    14941 tgttgccacc cgaaaaacgt tacgtttgaa aggtcttctt taacaatgat gacagccatc
    15001 actggctgtc agattgaaaa tttagtggtt aacgaattcc ccatccagat aaactttgcc
    15061 tttttccaca tgcatgacta aaccatccag ataatcaggt ttaaatgtcg catcacgctt
    15121 atttttagca atatatgtca ggcaatgatt atgggtgaaa ataacgatat ttttatcagg
    15181 cgctttgctt tgtaagtcct taattgcact ataaatctca ttaccgcact gaagaagtcg
    15241 tttatctacc gtcagttttt tacccgcgga aaaccaggtc gccgactgaa tggtccggac
    15301 ggtattactg gaataaagat cgaaatcagg gatgtcagcg ctaaaagcgt tgcccagttc
    15361 acgggcatcc tgggtacctt taaccgtaat acctgtttta tctgacaagc attgattggt
    15421 tgaacgatcg caacgttcag catggcgaaa caaaacgaca accgggtgct gctgtgccag
    15481 tctggccaat gttttattgt cgatacgtgg caaaccattg ctactccagg cggcatgagt
    15541 acccagtccg gcaattgcag cgagcgccag taaaatgatg aaatattttt ttgacttcaa
    15601 tgatgagcgg caaaaagcta acacgtaaac tccacctata gacaagcgca accagacaat
    15661 taccgtgaaa ttgagcgaca tttctggcga taattagcag ttggtgtaat attaaaaatc
    15721 ctacgatgtc ggcaaaatgc ctcaaaattt tgccaaatgc aaagcctaaa taagaaaaaa
    15781 tataaaaatt tcaatattta cgtctaatat tagtttctta aggttaagtt aatattctat
    15841 ccttaaaatt tcgctccaaa tggcaaaata tacacaacac tctttatagc aaatataagt
    15901 ggacaggtat tcaatggcgg aaggaaaagc aatgtcagaa tttttgcctt tttcgcgacc
    15961 agcaatgggc gtggaggaac tcgctgcagt taaagaggtt ctcgaatccg gttggatcac
    16021 aaccggtccg aagaatcagg cgcttgagca agccttttgc cagttgacgg gaaatcagca
    16081 tgccatcgcg gtcagttcag ccaccgccgg aatgcatatc acgctaatgg cgttggaaat
    16141 tggcaagggc gatgaagtga ttacgccttc cctgacctgg gtttcaaccc tcaatatgat
    16201 ttccttgttg ggtgcaacgc cggtaatggt ggatgtcgac cgcgatacgc tgatggtcac
    16261 gcctgaagcg atcgagtcag ccattacgcc acgcactaaa gccatcattc cggtgcatta
    16321 tgccggtgcg ccagcagata ttgacgccat tcgcgccatt ggcgaacgtt acggcatcgc
    16381 agttatcgaa gatgctgccc atgccgtcgg tacgtattac aaagggcgac atattggcgc
    16441 aaaaggtacc gctatttttt catttcatgc cattaaaaat attacctgtg cggaaggtgg
    16501 cctgattgta actgataatg aaaaccttgc ccgccagcta cggatgctga aatttcacgg
    16561 tctgggtgtc gatgcctatg acagacaaac ctggggccgt gcaccgcagg ctgaagtctt
    16621 aacaccgggc tataagtaca atctgaccga tattaacgcc gcgattgccc tgacacagtt
    16681 agcaaaatta gagcacctca acacccatcg gcgcgaaatt gcccagcaat atcagcaagc
    16741 actggcagct ctcccctttc agccattaag ccttcccgcc tggccgcacg ttcacgcctg
    16801 gcatctgttt attattcgtg tcgatgaaca acgttgtggt atcagtcgcg atgcgttgat
    16861 ggaagcgtta aaagaaagag gcattggtac cgggttacat ttccgcgccg ctcacacaca
    16921 aaaatattat cgcgagcgtt ttcccacgct gtcgttaccg aataccgaat ggaatagcga
    16981 acgcatctgt tctttgccgc tgttcccgga tatgactacc gccgatgccg accgtgtcat
    17041 cacagccctt cagcaactcg caggacaata agccatgttt gaaatccacc ctgttaagaa
    17101 agtctcggtg gttattcccg tttataacga gcaggaaagc ttaccggaat taatcaggcg
    17161 caccaccaca gcctgtgaat cgttggggaa agagtatgag atcctgctga ttgatgacgg
    17221 cagtagcgat aattccgcgc atatgctggt cgaagcctca caagcggaga acagccatat
    17281 tgtgtctatt ttgcttaacc gcaattacgg gcaacattca gcgattatgg cgggattcag
    17341 tcacgttact ggcgacttaa ttattaccct tgatgccgat ctccagaatc cgccagaaga
    17401 aatcccccgc ctggtggcaa aagccgatga aggttacgac gtggtaggga ctgtacgcca
    17461 gaaccgccag gacagctggt ttcgtaaaac agcttcgaag atgattaaca ggcttattca
    17521 gcgcaccacc ggcaaagcga tgggtgacta cggttgtatg ctgcgcgcct atcgccgtca
    17581 tattgtcgat gcgatgttgc actgccatga acgtagcacc tttatcccga ttctggcgaa
    17641 tatcttcgcc cgccgtgcca ttgaaattcc agtacatcat gccgagcgtg agtttggtga
    17701 atccaaatac agttttatgc gcctgattaa tttgatgtac gacctggtga cctgccttac
    17761 cacaacgccg ctacgtatgc tgagtctgct cggcagcatt attgcgattg gaggttttag
    17821 cattgcggtg ctgctggtga ttttacgcct gaccttcgga ccacaatggg cggcagaagg
    17881 cgtctttatg ctatttgccg tgctgtttac ttttattggc gctcagttta tcggcatggg
    17941 attgctcggt gaatatatcg gcaggatcta caccgatgtc cgcgcccgcc cccgctattt
    18001 tgttcagcaa gttatccgtc catccagcaa ggaaaatgaa taatgaaaac cgtcgttttt
    18061 gcctaccacg atatgggatg cctcggtatt gaagctctgc tggctgccgg ttacgaaatt
    18121 agcgccattt ttacccatac cgataatccc gctgaaaaag ccttttatgg ttcggtggct
    18181 cgtctggcgg cggaaagagg cattccagtt tatgcgccgg ataacgttaa tcatccgctg
    18241 tgggtggaac gcattgccca actctcacca gaggtgattt tctcttttta ttatcgccat
    18301 cttatttgcg acgagatttt gcagctcgct cccgcaggtg catttaatct gcacggttcg
    18361 ctgttaccaa aatatcgtgg tcgcgcgccg ctgaactggg tgctggtgaa cggtgaaacg
    18421 gaaacgggcg ttacattgca ccgaatggta aaacgtgccg atgctggggc cattgtggcg
    18481 caactgcgcg ttgccattgc gccagacgat atcgctatta cgctgcatca taaattgtgc
    18541 catgccgcgc gccagctact ggaacagaca ttacccgcca ttaaacacgg taatattctg
    18601 gaaattgccc agcgcgaaaa cgaagccacc tgttttggtc acagaacgcc agatgacagt
    18661 ttccttgaat ggcataaacc ggcatccgta ctgcacaaca tggtacgtgc cgttgccgat
    18721 ccgtggccgg gtgccttcag ctatgttggc aatcagaaat tcaccgtctg gtcgtcgcgt
    18781 gttcatcctc atgccagcaa agcacagccg gggagcgtga tttctgttgc gccactgctg
    18841 attgcctgtg gcgatggcgc gctggaaatc gtcaccggac aggcgggcga cggcattact
    18901 atgcagggct cgcaattagc gcagatgctg ggcctggtgc aaggttcacg cttgaatagc
    18961 cagcctgcct gcaccgcccg acgccgtacc cgggtactca tcctcggggt gaatggcttt
    19021 attggcaacc atctgacaga acgcctgctg cgcgaagatc attatgaagt ttacggtctg
    19081 gatattggca gcgatgcgat aagccgtttt ctgaatcatc cgcattttca ctttgtcgaa
    19141 ggcgatatca gtattcattc cgaatggatt gagtatcacg tcaaaaaatg tgatgtcgtc
    19201 ttgccgctgg tggcgatagc cacgccgatt gaatataccc gcaacccgct gcgcgtattt
    19261 gaactcgatt ttgaagagaa tctgcgcatt atccgctact gcgttaagta ccgtaagcga
    19321 atcatcttcc cgtcaacttc agaagtttat gggatgtgta gcgataaata cttcgatgag
    19381 gaccattcta atttaatcgt cggcccggtg aataaaccac gctggattta ttcggtgtca
    19441 aaacaattac ttgatcgggt gatctgggcc tatggcgaaa aagagggttt acagttcacc
    19501 ctcttccgcc cgtttaactg gatgggacca cgactggata accttaatgc agcgcgaatt
    19561 ggcagctccc gcgctattac gcaactcatt ctcaatctgg tagaaggttc accaattaag
    19621 ctaattgatg gcggaaaaca aaaacgctgc tttactgata tccgggacgg tatcgaggcg
    19681 ttgtaccgca ttatcgaaaa tgcgggaaat cgctgcgacg gcgaaattat caacattggc
    19741 aatcctgaga acgaagcgag cattgaggaa ctgggcgaga tgctgctggc gagcttcgaa
    19801 aaacatccgc tgcgccatca cttcccgccg tttgcgggct ttcgtgttgt cgaaagtagc
    19861 agctactacg gcaaaggata tcaggacgta gagcatcgta aaccgagcat ccgcaatgcc
    19921 cgccgctgcc tggactggga accgaaaatt gatatgcagg aaaccatcga cgaaacgctg
    19981 gatttcttcc tgcgcaccgt tgatcttacg gataaaccat catgaccaaa gtcggcttac
    20041 gcattgatgt cgataccttt cgtggcaccc gtgaaggcgt gccacgtctg ctggaaatct
    20101 tgagtaagca taatattcag gccagcattt ttttcagcgt cggcccggac aatatgggcc
    20161 gccatctctg gcgactggtg aagccacagt ttttgtggaa gatgctgcgc tcaaacgcgg
    20221 catcgcttta tggctgggat attttactgg caggtacggc ctggccaggt aaagagattg
    20281 gtcatgccaa tgccgatatc attcgtgaag cggctaaaca tcacgaagtc ggcctgcacg
    20341 cctgggatca ccatgcctgg caagcccgta gcggtaactg ggatcggcaa acaatgatcg
    20401 acgatattgc acgcggtctt cgcactctgg aagagattat cggtcaaccg gtaacctgtt
    20461 ctgccgctgc gggctggcgt gccgaccaga aggtgatcga agcaaaagaa gcgttccatt
    20521 tgcgctacaa cagcgattgt cgtggggcca tgccgttccg tccactgctc gaatcaggaa
    20581 accctggcac tgcgcaaatt ccggtgacct tacccacctg ggatgaagtg attggtcggg
    20641 atgtgaaagc agaagatttt aacggttggt tactcaaccg catcctgcga gataaaggca
    20701 cgccggttta taccattcat gcagaagttg aaggctgcgc ttatcagcat aattttgtgg
    20761 atctcctcaa acgcgcagct caggaaggcg tgacattttg ccctttaagc gaactgttat
    20821 cagagacgtt gccgctcgga caagttgttc gcggaaatat tgccggacgt gaaggctggc
    20881 tgggttgcca acaaattgcg ggtagtcgct gatgaaatcg gtacgttacc ttatcggcct
    20941 cttcgcgttt attgcctgct attacctgtt accgatcagc acgcgtctgc tctggcaacc
    21001 cgatgaaacg cgttatgcgg aaatcagtcg agaaatgctg gcatccggcg actggattgt
    21061 gccccatctg ttagggctac gttatttcga aaaacccatt gccggatact ggattaacag
    21121 cattgggcaa tggctatttg gcgcgaataa ctttggtgtg cgggcaggcg ttatctttgc
    21181 gaccctgtta actgccgcgc tggtgacctg gtttactctg cgcttatggc gcgataaacg
    21241 tctggctcta ctcgccacag taatttatct ctcattgttt attgtctatg ccatcggcac
    21301 ttatgccgtg ctcgatccgt ttattgcatt ctggctggtg gcgggaatgt gcagcttctg
    21361 gctggcaatg caggcacaga cgtggaaagg caaaagcgca ggatttttac tgctgggaat
    21421 cacctgcggc atgggggtga tgaccaaagg ttttctcgcc cttgccgtgc cggtattaag
    21481 cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat ctctttattt acggctggct
    21541 ggcggttatc agttgcgtac tgacggttct cccctgggga ctggcgatag cgcagcggga
    21601 gcctgacttc tggcactatt ttttctgggt tgagcatatt caacgctttg cactggatga
    21661 tgcccaacat agagctccgt tctggtacta cgtgccggtc atcattgccg gtagcctgcc
    21721 gtggctggga ttactccccg gtgcactgta cacaggctgg aaaaaccgca agcattccgc
    21781 aaccgtctat ttgttgagct ggacgataat gccgctgctg tttttctccg tcgctaaagg
    21841 taaattgccc acctatattc tttcctgctt tgcatctctg gcaatgctga tggcgcatta
    21901 cgctttgctg gcagcaaaaa ataatcctct ggcgctgcgg attaatggct ggattaacat
    21961 cgcttttggc ttcactggca ttattgccac atttgtggtc tccccgtggg gaccaatgaa
    22021 cacgccggtg tggcaaacct tcgagagcta taaagtcttt tgtgcctggt cgattttttc
    22081 gctatgggca tttttcggct ggtacacctt aacaaacgtc gaaaagacct ggtcttttgc
    22141 cgcgctttgc ccgctggggc tggcgttgct ggtaggattt tcaattcctg acagagtcat
    22201 ggaaggaaaa catccgcaat tttttgtcga gatgacacaa gaatcacttc agccaagccg
    22261 ctatattctt actgatagcg tcggtgttgc cgcaggtctg gcatggagcc tgcaacgcga
    22321 tgacatcatc atgtatcgcc agacaggtga gttgaaatac ggccttaatt atccggatgc
    22381 gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg cttaatcaac atcgtcagga
    22441 ggggattatt acactcgtgc tttcggttga ccgcgatgaa gatatcaaca gtctcgccat
    22501 tccgcccgca gatgccatcg atcgtcagga gcgtctggtg ctgattcagt atcgtcccaa
    22561 atgatctggc taacattagt ctttgccagc ttgcttagcg ttgccgggca gttgtgtcag
    22621 aaacaggcaa cctgctttgt ggcgataaac aaacggcgca aacatatcgt gctgtggctg
    22681 ggactggcgc tggcttgtct tggtcttgcc atggtgctct ggctgctggt cttgcagaac
    22741 gtaccggtag gcattgccta cccgatgtta agtctgaatt ttgtctgggt gacgctggct
    22801 gcagtaaaac tgtggcacga accggtatcg ccgcgtcact ggtgtggggt ggcgttcatt
    22861 attggcggca ttgtgatcct cgggagtacg gtgtaatggg cctgatgtgg ggattattca
    22921 gcgtcattat tgcctcagtt gcgcaattaa gcctgggttt tgcggcgagt catctgccgc
    22981 cgatgacgca cttatgggat tttatcgcgg ctctgttagc ttttggcctg gatgcccgga
    23041 tactgttact cgggctgctg ggttatctgc tttccgtttt ttgttggtat aaaacgctac
    23101 ataaacttgc gctaagcaaa gcctatgcct tgctcagtat gagttatgtg ctggtatgga
    23161 ttgcttcaat ggtacttccc ggctgggaag ggactttttc gctgaaagca ctactgggag
    23221 tagcctgtat tatgagcggg ttgatgctga ttttcctgcc cacgacaaaa caacgttact
    23281 gagttttccc tgccacttta cactgccgtt cccactcatc ggggctataa ctgcttgccg
    23341 agaggacttt caccgtatgc agcttttcac gattaataca ataaagcgca ttctgcaaag
    23401 gcgagagtaa atcccccact ttcacggcga agtcactctt cacttcggcg atcattttta
    23461 tcgcgccgcc agcatcgcag agcataagta catgtctgtt atcttgttta ttgcagcacg
    23521 attttttgac cagccattcc attgcattat cctgtttgct aagagttttc acatcaattt
    23581 acagattaac agataaacct attatcaacg gcaatggaat ggcaaaattg cgaagtgaaa
    23641 ctaatgattt cgtaatcctg gcgttgcacc cactctttta acgcctgacg tgaaatttta
    23701 ataccgccgt ttttcagctc cggcggtaaa gttagccagc gcaccggttg ttgaaaacga
    23761 gccagcttat ctttcaccca ttcgccaaga tcaacggttt gctggtcata ctccaccacc
    23821 gctaccggtc gatgaccaaa ctccttgtcg gcaacgggga cgataaacac ctgcaaaacc
    23881 gcaggatgtg cagcaattac gcgctcgact tcttccggct gaataccctc tccgccactg
    23941 aagaacagat tgtctaaacg tccgacaatg gtcagcttgc cattatgcat ctcaccgcga
    24001 tcgcgcgtag cgtaccagcc ttcgtcatta accagtgaaa ccagttgccc gttacgccag
    24061 taaccttctg ccatactggc agcccgcagc cacacttcat tattaacgat tttcacttcc
    24121 cgacccggca gcggcgaacc aacgtctgcc aggccgtcgg cttctttcgc acacaccgtg
    24181 gaggcaaact cggtcagacc atagccgcaa aagcaacgaa tcccctgctc gcgcgcctgt
    24241 tccgtcaact cgaccgggat agccgcgccg ccaagtaaca ccgctttcag ggaaacggaa
    24301 ctgcggttaa ccagcaaacg ccagagttgt gttggcacca gtgaagcgtg agtacagcct
    24361 gccagcattt gctccaatgg ctgtttatca cgcaccgtca tccgcgcacc agcgtataac
    24421 cagcgccaca taattccctg accggagacg tgaaacagcg gtaaagagag caaccagtca
    24481 tcgtgatcgc caaacggaat cagcgataac acaccttgcg cactggcaag atgggcctga
    24541 taagtatgga cagcggcttt cggcaaaccg gtagaacccg aggtcaacgt cattgagcac
    24601 agacgcgtcg gctgccacgt agcggcatgt gcgccttcaa cccgctgaat gtgcagcgac
    24661 gttaatgccg gaaacgtgtt ttccccatcc ggcaccagcg caaattgcag cgtcagattg
    24721 ggcagcaatt cttcaagcaa cggttgcggc agctgagggt tcacgggcaa cacccgcgcc
    24781 ccgcattgca gtaacgccag ccaggcgagc agcgtttgcg gcgtattcca cgcccgcaac
    24841 atcacgccgc tgccctcaac cacaccctgc gccgcaaagc ccgtagctaa ctcatcgacg
    24901 cgagcacaaa gctcgcgcca gttgagttgc tcgtcattaa gacgtaaggc gatggcttct
    24961 ccccgcactt gccgccagtg acgccacggc cagtcagaga agatcataac aactgctcca
    25021 gtacatcagc gtccactaac ggcagtgggc tccccggcca gcgacgaacc tgctgcgcct
    25081 gcatcagatc cagcgtgtcc agtcccggaa tggtgtctgg cgttaaccag gcagcaatcc
    25141 gcgccagttg cgttagacct aagctcgact caatggaaga actgatcacc gccgtcagcc
    25201 ccagcgcatg cgccgcctgt acctgctcgc gtactttttc cagactgccc gtgagcgtgg
    25261 gtttgataac taccgcgcgc acgccctctt cagccacaaa ggcaaaatcc ggctcgcgca
    25321 ggctttcatc ccaggcaatg gcaatgccgg tttcacgggc aaacgctcgc gaatcatcgc
    25381 gggttttgca cggctcttcg agaaacgcga tgcggtggcg ataatccggg ttaacatatt
    25441 tggcaaactg ctgacctttc agcggtgtcc aggcgcgatt tgcgtcaaga cgcaaatgca
    25501 gatccggaat tgcctccagc aacagattaa ccaccatgcc gtcgcgcacg gcttcgtaca
    25561 atcccacttt gaccttcgcc actttctcgc ctggcatatc agcaagtttg aggatcagat
    25621 cgtcaggatc gccattacac agtggtgccg cacggtaatt ggccgcctgc gggagcgtat
    25681 ctgccatctc cgccaatgca cagcttacgc caaaggcaac ggaaggcatc tgcggtagct
    25741 cgccatcgcc tgccagccag ttatttaccc aagcaagcag cacaatttgc gcctcttccc
    25801 aggtttcctg actgaagccc ggcagtgggg agatctcccc ccacccttcg cgctcgcctt
    25861 cacgcaggca aacatacagc ccgtcgcggg tttttaaccg cctgtcgcgc agaaccatcc
    25921 ccgcgtccat ggggatctgc cagcggtata cctgcgcgct acgcattacg gattccgttt
    25981 aaatttgctg aaatcaggct gacgtttctg gttgaaagcg ttgcgacctt cctgaccttc
    26041 ttccgtcatg tagaacagca tggtggcgtt gcccgccagc tcctgcagac ccgcctgacc
    26101 gtcacagtcg gcgttcagcg cagctttcag gcagcgcagc gccatcgggc tgttttgcag
    26161 catttcgcgg caccaacgga cggtttcttt ttccagatcc gccagcggta ccacggtgtt
    26221 gacaaggccc atatccagcg cctgttttgc atcgtactga cggcacagga accagatttc
    26281 acgcgctttt ttctgtccga cgatgcgagc catgtaggaa gcgccccagc cgccgtcgaa
    26341 ggaaccgact ttcgggccag tctgaccgaa gatggcatta tctgccgcga tagtcaggtc
    26401 gcacatcatg tgcagaacgt gaccaccgcc gatggagtag ccagccacca tcgcgacaac
    26461 cggtttcgga caggtacgga tctgacgctg gaagtccagc acgttcaggt gatgtacgcc
    26521 ggaatcatct ttatagccgc cgtaatcacc acgcactttc tggtcaccac cggagcagaa
    26581 cgctttatcg cctgcgccag tcagaatgat cacgccgacg ttgtcgtcat aacgcgcatc
    26641 tgccagtgcc tggatcatct cttttaccgt cagaggacgg aaggcattgc gcacctgcgg
    26701 acgattaatg gtgatttttg cgataccgtc ggtggatttt tcataacgaa tgtcctcaaa
    26761 accttcggag cagtcgtgcc attcaaccgg tgcgtaaagc attgcttcat caggataaat
    26821 catattgtgt cctttagtca gaaacgcaag atctgcgcca gacttgcgat aacgccagcg
    26881 ggattttccc gatgcgcgtt atgtccggcg cgaggaatga catggcagtc ggcagccagt
    26941 tccgccgcca aggcgcggaa tttgctgtca cgttcaccac ataaataata aaacgcaaat
    27001 gtgcgggcgc taaggttagc acgtaaatca ggctggacgg cgagagaagt cgcctccagc
    27061 atggcggcaa gggttgcgcc attattgtta ctgcgcagcg ccaccagctc ccggcgttga
    27121 tcgtcattga gcgaggcaaa aacaggctgt tgataccagt cggcaaatac cgccgttaac
    27181 ggttctgtgc gaaaacgctg cgcccattgg cgatcggaac gctgacgttc cgcgcgttgt
    27241 tcagcatttt gcaacccagg atgcccgcct tcgacaacaa ccccacaaag ccccggcatc
    27301 ccctggcaag ccgccatcat cgccacccgt cccccaagcg agtaccccac caaccagaag
    27361 ttaaggatgt tgtaactaac cagggtttta cgcagtaagt cggtgacatc atcaaatcca
    27421 tcgacgctaa tcgccgccga accaccgtga cctgggagat aaacatacaa ccgtgagtag
    27481 tcggcaaacg cctcgcccac ttcttgccat tcgtggcaat cgccggaaaa accgtgcaaa
    27541 aacaccagcc agggtaaacc tggtttgccg tgttttgcct gcgcgtgcag gatcataaat
    27601 ggcttacctg cgccagaagt tgctggagcg tttgcgcacc atcggtgtcg ttaaccacca
    27661 tttcaattac cgtggtggtt ggcgtgcgcc aggcatcggc aagtgccgtt tcaagttcct
    27721 gccagttttg cggacgatga tatttcagct cgaacatcgc ggcggcgtgc tcaaaatgga
    27781 cgttttgcgg catcagatag aaacgctcgc gctcgctttt cggcgttggc agcagcgaga
    27841 aaatttgccc gccgttgttg ttcaccacaa ttaatatcag cggcgcggaa acctgacgca
    27901 ataacgccag cgcgttgaga tcgtaaagtg cggagagatc gcccacaatc gccagcgtcg
    27961 gtttgccgct tgcccgctga acgccggcgg cagtcgagag cagcccgtcg ataccgctgg
    28021 ccccacggtt gctgtacacc gggtaacctg ccggaagttg cgaaagcgca tcaatcagac
    28081 gtaccaccag gctgttaccg acaaacaatt gcccctgttc aggcagatag tcgctgatgc
    28141 gatgcgccag ttgcgcttcg ccaaacgcat cgcgacgggc aataaccgcc tgcattgcct
    28201 gttccgccag gcgcgggatt tcaacgcacc agggctggcg tttttctgcc ggatgcagct
    28261 ccagccagtc ggcaatattg gcaattaagc ggcgtccacg atggtgcgcc ggatcaagcc
    28321 gcccttcgat gtcatccaca atccagtact cttcgggttc acagcttgcc tgccattgca
    28381 ggagccgttt gcccgtcagg ctgcttccca gttgcaccac aatttgcgcc tgttgcagtt
    28441 cgctggtcgc tttggcattg cctaaccaga gatcggcaca cggcagcggc tgtccggttt
    28501 gcgacagcac gtcgccaatc agcggccagc caagagtttg cgcccacagt gcaacttttt
    28561 tgccctcttc cgcactcatg cgcccggcaa ccaccacgcc gcgtttttgt cgccagaaga
    28621 accagtcgcg ctgtttttca ctttccagac gaggcgcttc acgcagccac ggtttgtcgt
    28681 cctgccacca gtcgcccagc cgctgttgcc agctaagccc ggtgtcgtcc atttcgccat
    28741 acagcggttc ggcaaacggg cagttgatat ggactccacc agaatgaagc gtaccgagag
    28801 catggtcgat ggtagaaacc agccaacgtg cggggatatc ctgggtcggg cgcggcaatg
    28861 aaatactgtg cgtggggtga gaggcgaaca ttcccggctg acgaatcgcc tgattcgcgc
    28921 cgcagtcaat cagctccggc gggcgatcgg cggttaacag gatcagtttt tctccggtta
    28981 acccagcttc aatcagtgcc ggatagagat ttgctaccgc cgtaccggag gtcacaatca
    29041 ccgccaccgg ctgcttgctg actttcgcca gccccagcgc cagatgcccc aacccacgct
    29101 catcgaaatg ggtgtggtga atgaatgcgg aattctccgc cgccgctaac gttaacggtg
    29161 tagaacgcga gccaggggcg atacagatgt gtctgacgcc gtgacgcgtt aatgcttcca
    29221 gaatgaccgc cgcccagcgt cggttaaatg cgcttactga catgagtttg tccggtatca
    29281 atattgcggc taagtataag gagctacaaa aatagattat tgatatgaat cggtaatgat
    29341 gcgactcatt actattccat ctgtaataaa gtacgcagcc ctgccgcttt gttgtcgatt
    29401 tcctgccact cttgctcggg gtcggaacca cggacaatgc ccgcgccagc atataatcgc
    29461 acgacattgc cgctaatttt tgctgagcgc agggaaacgc agaattcgct ttgctgtaat
    29521 gagagatagc ccgcagaacc ggcgtaccat tctcgggtga acggttcgtg acgggcgata
    29581 aactgtcgcg ccagatcgcg cggtaagcca gcaactgctg ccgttggctg caactgatgt
    29641 aaacagatca cattatccgc tttgttgagt gaagtccaga tacagcggcg aagatgctgc
    29701 actttacgca gacgcagtac ctgcggcggt aaaacatcca gcgtctgggt atcggcctgt
    29761 aaccgttggc agatatcttc caccaccagc atattctcgc gctggttttt atcatccgcc
    29821 atcagccact cacctaactg ctgcgcctgc ttatcatcag gatgatttgc taccgttccc
    29881 gccagcgctt cagtacgcag cgctttgtca cgccgccgcc ataaccgttc cggtgaagag
    29941 ccaagaaaag cattttcgcc atcaaaggcc atgtaaaaat ggtagcaatt cagattcagt
    30001 cgacgactgg cagccatcat cgccgccgcg ttgaccggac ttgcgaaatg caggtcagtt
    30061 gcccgagcga gcaccacttt gtcgagctca ccttcggcga tggttttcgt tgccagttcg
    30121 attaattgcg tccagcccgt tttgtccggc cagtgttgtt ctcgcgtggt ggttaaatgt
    30181 aacccaggca agggcttgat actcaccagt gtggcgataa attctcttgc cttaatcgca
    30241 tcgtgctgaa gggagctttc gctgaatagc gtcagccgca gcgtggcttt accgccacag
    30301 cgtcgccatt ccaggcgtgg taaaagtaaa ttgccctgcg acgggtcaaa cgcattcagc
    30361 ccccaaatgc gtaagtcggc gtgttccggg tgctggcgaa gaaaacgttg tgcctggtcc
    30421 aacgacgtaa aacgggtaat cgcgcccagg acggcagctt cttcatcacc attacgttgt
    30481 tgccagtaga attgcgggta tgtttgctga ctggccagcc agctcaaggc atcaaaagcg
    30541 tctttgagag ggaaaggaat atcgataacc cgaatgccgg gtgtcgccgg aatctcttgc
    30601 gacaaatggc gcagtagatt ttccagcgcc gtagtaagtg attgcacgcg gacctctccc
    30661 tgttaaaagc ctcacattat acggggtact acaaaaaaaa tgcagtaccc cggtgtaggg
    30721 aggttttaac ggcgtgccag caacagtcct agtaccagcc caacggccgc gcccacacca
    30781 attccttgcc agggtttttc gtggacgtag tcatcagcac gataaactgc ctgcttcgcc
    30841 cgatagtaat aactgtctga agcctggcta acccgttttt tcacatcatc cagcgctttt
    30901 tctgcacgcg ctttcagctc aacatatttc tgatcggcgg gatcgccaga ggagcggagc
    30961 acctcttcca gtgtttcact aagcagcgtc aggtcgtcat cgatacgtgt atcaccaaac
    31021 tgattagaca ttctcgttct ccatttgcgt aaaacctgtc actaactata gacaagggtt
    31081 gttgattacg cctgaattac ctcgcgtgcc atcccaatgt gtgggatacc atcttcttca
    31141 tagacctccg tcacagggat aaaaccgaaa ctctggtaga agttttgcag atgcgcctgc
    31201 gccccgaggt aaacaggctt atcaggccag tgatgcgtgc aggtgtcgag ggttttactc
    31261 atcagttgct gccccacttt ttcgccacgc aacgcttcac tgacaatcac ccgaccaata
    31321 acgaccggct caagatcatc atcacttttc agaatcctcg catatgccac cagttcatca
    31381 tttttccaac cgagaatatg gcgattatcc cccgtcaaat catcaccatc gatatcctga
    31441 taagggcaat tctgctcgac aacaaacacc gcgcaacgca gttgtaataa ggcataaagc
    31501 tgggaaaccg ataattcaga gtgatgcaga tcttaccatt caatcataat gctctccttt
    31561 tagatgtatg ccttatacta gccggaatat tttttgaaac gtgatgaact catggaatta
    31621 atttttttag gtacttcagc cggtgtgcca acccgcacgc gcaatgtcac ggcaatattg
    31681 cttaacctgc aacatccgac ccagagcgga ctttggttgt ttgactgcgg tgaaggcacc
    31741 cagcatcagc tactgcatac cgcctttaac cctgggaaac tggacaagat ttttatcagt
    31801 caccttcatg gcgatcatct ctttggttta cccggcttgc tgtgcagtcg ttctatgtca
    31861 ggcattatcc aacccttaac gatttatggt ccgcaaggta tccgtgaatt tgtggaaacc
    31921 gcgctgcgga ttagcggctc atggaccgat tatccgctgg aaattgtcga aattggcgct
    31981 ggcgaaattc tcgatgatgg cctgcgcaaa gtgaccgctt atccgatgga acacccactg
    32041 gaatgttatg gctatcgtat tgaagaacat gataaaccgg gtgcattaaa tgcccaggca
    32101 ttaaaagctg ctggcgtgcc gcctggccca ctgtttcagg aattaaaagc gggcaaaaca
    32161 atcacgctgg aagatggaag gcagattaac ggcgcagatt acttagctgc tccagtgcca
    32221 ggtaaagcgc tcgctatttt cggcgatacc ggcccctgcg atgccgcact tgacctggct
    32281 aaaggtgtcg atgtcatggt gcacgaagcg acgctggata taaccatgga agccaaagcc
    32341 aatagtcgcg gccatagctc tacacgccag gctgcgacac tagcccgtga ggctggagtc
    32401 ggcaagctaa tcattaccca cgtcagctcg cgctatgatg acaaaggttg tcaacacctg
    32461 ttacgtgaat gcaggtcaat tttcccggcg actgaactgg cgaatgattt caccgtgttt
    32521 aacgtttaac tc
//
LOCUS       DAZPAC010000023        32398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZPAC010000023 DAZPAC010000000
VERSION     DAZPAC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.023"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            157..720
                     /locus_tag="ACHUPM_000892"
     CDS             157..720
                     /locus_tag="ACHUPM_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0868753.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            802..3444
                     /gene="yfcU"
                     /locus_tag="ACHUPM_000893"
     CDS             802..3444
                     /gene="yfcU"
                     /locus_tag="ACHUPM_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFR0868754.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            3464..4215
                     /locus_tag="ACHUPM_000894"
                     /pseudo
     CDS             3464..4215
                     /locus_tag="ACHUPM_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            4190..4738
                     /locus_tag="ACHUPM_000895"
     CDS             4190..4738
                     /locus_tag="ACHUPM_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0868755.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4735..5205
                     /locus_tag="ACHUPM_000896"
     CDS             4735..5205
                     /locus_tag="ACHUPM_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0868756.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5202..5726
                     /locus_tag="ACHUPM_000897"
     CDS             5202..5726
                     /locus_tag="ACHUPM_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0868757.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            5713..6585
                     /locus_tag="ACHUPM_000898"
     CDS             5713..6585
                     /locus_tag="ACHUPM_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR0868758.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(6707..7258)
                     /gene="smrB"
                     /locus_tag="ACHUPM_000899"
     CDS             complement(6707..7258)
                     /gene="smrB"
                     /locus_tag="ACHUPM_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR0868759.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7424..8356
                     /gene="prmB"
                     /locus_tag="ACHUPM_000900"
     CDS             7424..8356
                     /gene="prmB"
                     /locus_tag="ACHUPM_000900"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0868760.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            8391..9476
                     /gene="aroC"
                     /locus_tag="ACHUPM_000901"
     CDS             8391..9476
                     /gene="aroC"
                     /locus_tag="ACHUPM_000901"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR0868761.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9480..10304
                     /gene="mepA"
                     /locus_tag="ACHUPM_000902"
     CDS             9480..10304
                     /gene="mepA"
                     /locus_tag="ACHUPM_000902"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR0868762.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10304..11113
                     /locus_tag="ACHUPM_000903"
     CDS             10304..11113
                     /locus_tag="ACHUPM_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFR0868763.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            11113..11661
                     /gene="epmC"
                     /locus_tag="ACHUPM_000904"
     CDS             11113..11661
                     /gene="epmC"
                     /locus_tag="ACHUPM_000904"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR0868764.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11695..11973
                     /locus_tag="ACHUPM_000905"
     CDS             11695..11973
                     /locus_tag="ACHUPM_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR0868765.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(12094..14100)
                     /gene="mnmC"
                     /locus_tag="ACHUPM_000906"
     CDS             complement(12094..14100)
                     /gene="mnmC"
                     /locus_tag="ACHUPM_000906"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR0868766.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            14259..15479
                     /gene="fabB"
                     /locus_tag="ACHUPM_000907"
     CDS             14259..15479
                     /gene="fabB"
                     /locus_tag="ACHUPM_000907"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR0868767.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15754..16932
                     /locus_tag="ACHUPM_000908"
     CDS             15754..16932
                     /locus_tag="ACHUPM_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0868768.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(16929..17924)
                     /gene="flk"
                     /locus_tag="ACHUPM_000909"
     CDS             complement(16929..17924)
                     /gene="flk"
                     /locus_tag="ACHUPM_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR0868769.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            18023..19159
                     /gene="pdxB"
                     /locus_tag="ACHUPM_000910"
     CDS             18023..19159
                     /gene="pdxB"
                     /locus_tag="ACHUPM_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR0868770.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            19225..20238
                     /locus_tag="ACHUPM_000911"
     CDS             19225..20238
                     /locus_tag="ACHUPM_000911"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0868771.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            20238..21050
                     /gene="truA"
                     /locus_tag="ACHUPM_000912"
     CDS             20238..21050
                     /gene="truA"
                     /locus_tag="ACHUPM_000912"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR0868772.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            21133..21792
                     /locus_tag="ACHUPM_000913"
     CDS             21133..21792
                     /locus_tag="ACHUPM_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0868773.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            21948..22862
                     /gene="accD"
                     /locus_tag="ACHUPM_000914"
     CDS             21948..22862
                     /gene="accD"
                     /locus_tag="ACHUPM_000914"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR0868774.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            22932..24200
                     /gene="folC"
                     /locus_tag="ACHUPM_000915"
     CDS             22932..24200
                     /gene="folC"
                     /locus_tag="ACHUPM_000915"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR0868775.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            24190..24852
                     /gene="dedD"
                     /locus_tag="ACHUPM_000916"
     CDS             24190..24852
                     /gene="dedD"
                     /locus_tag="ACHUPM_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR0868776.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            25111..25599
                     /gene="cvpA"
                     /locus_tag="ACHUPM_000917"
     CDS             25111..25599
                     /gene="cvpA"
                     /locus_tag="ACHUPM_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR0868777.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            25636..27153
                     /gene="purF"
                     /locus_tag="ACHUPM_000918"
     CDS             25636..27153
                     /gene="purF"
                     /locus_tag="ACHUPM_000918"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR0868778.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            27248..27817
                     /gene="ubiX"
                     /locus_tag="ACHUPM_000919"
     CDS             27248..27817
                     /gene="ubiX"
                     /locus_tag="ACHUPM_000919"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR0868779.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            28083..28865
                     /gene="argT"
                     /locus_tag="ACHUPM_000920"
     CDS             28083..28865
                     /gene="argT"
                     /locus_tag="ACHUPM_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR0868780.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            29086..29868
                     /gene="hisJ"
                     /locus_tag="ACHUPM_000921"
     CDS             29086..29868
                     /gene="hisJ"
                     /locus_tag="ACHUPM_000921"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR0868781.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            29958..30644
                     /gene="hisQ"
                     /locus_tag="ACHUPM_000922"
     CDS             29958..30644
                     /gene="hisQ"
                     /locus_tag="ACHUPM_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR0868782.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            30641..31357
                     /locus_tag="ACHUPM_000923"
     CDS             30641..31357
                     /locus_tag="ACHUPM_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0868783.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            31365..32138
                     /gene="hisP"
                     /locus_tag="ACHUPM_000924"
     CDS             31365..32138
                     /gene="hisP"
                     /locus_tag="ACHUPM_000924"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR0868784.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            32335..>32398
                     /locus_tag="ACHUPM_000925"
     CDS             32335..>32398
                     /locus_tag="ACHUPM_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868785.1"
                     /translation="MTISTTSTPHDAVFKSFLRHP"
BASE COUNT         7597 a         8017 c         8752 g         8032 t
ORIGIN      
        1 actatgcggc cccacaccaa cgaatacaat tttctacgac ctgttctttt tggatcgcat
       61 gccgtattca atggctgggt ataaacattg gttattgact gaatcaatgt attgcaatgt
      121 ttatattttt aattatttat agtttaagga caaataatga gtaagtttgt taaaacagct
      181 attgctgcgg caatggtgat gggcgtgttc acctctacgg caacaatcgc tgcgggtaac
      241 aacggtactg cacgttttta cggcaccatt gaagactcag tgtgttctat cgttccggac
      301 gatcacaaac tggaagttga tatgggtgat atcggtgccg aaaaactgaa aaataacggc
      361 accaccacgc cgaaaagttt ccagattcgt ctgcaagatt gcgtatttga tactcaggaa
      421 acaatgacca ctacctttac tggtaccgtt tcttctgcaa atagcggcaa ttattacacc
      481 attttcaata ccgatacctg tgcggcattt aacaatgtta gcctggcgat cggtgactct
      541 ctgggtacct cttacaaaag cggcatgggt attgaccaga aaatagtgaa agacacttct
      601 accaataaag gcaaagcgaa gcagacactg aactttaaag cctggctggt cggcgcagct
      661 gatgcgccag atctgggtaa tttcgaagca aataccacct tccagattac ttacctgtaa
      721 ggcatacccc catagcgaca ctgctatggg ggatttaaat ggattttgac cgaacaagaa
      781 taaaaacgga tagaaacgtg tatgcctgac cattctcttt ttcgattacg gattcttccg
      841 tggtgcattg cgctggcaat gtcagggagt tatagcagtg tctgggctga agacgacatt
      901 cagtttgatt cccgttttct ggaattaaaa ggcgacacaa aaattgatct gaagcgtttt
      961 tccagccagg gatatgttga gcccggaaaa tacaatttac aggttcaact aaataaacag
     1021 ccattggcgg aagagtacga tatttactgg tatgctggtg aagatgacgc gagcaaaagc
     1081 tatgcttgtc tgacaccgga actggtagcg cagtttggtt taaaagaaga cgtggcgaaa
     1141 aatctgcaat ggagccacga tgctaaatgc ctgaaatccg gtcaactgga aggcatggaa
     1201 attaaggctg atttaagcca gtccgcatta gtcatttcac tgccacaggc ttacctcgaa
     1261 tatacttatc ccgactggga tccgccttca cgttgggatg acggcatctc cgggatcgtc
     1321 gcggactaca gcatcaacgc acaaacccgg cacgaagaaa atggcggtga tgatagtaac
     1381 gagatcagcg gcaacgggac ggtcggggtt aacctggggc cgtggcgtat gcgtgctgac
     1441 tggcagacta actatcaaca tactcgcagt aatgatgacg atgaattcag cggcgatgaa
     1501 actcaaaaaa aatgggagtg gagtcgctac tatgcctggc gggcgttacc atcattaaaa
     1561 gccaaactgg cgctgggcga ggattacctc agatccgata tttttgatgg ttttaactat
     1621 gttggtggca gtgtcagtac tgacgatcaa atgttgcctc ccaatctgcg cggctacgcg
     1681 ccagacattt ccggcgtggc acacaccaca gcaaaagtga ccgtcagcca gatggggcgt
     1741 gtgatttacg aaacgcaggt tccggctgga ccgtttcgta ttcaggatct tggtgattct
     1801 gtctccggta cgttgcatat tcgcattgaa gaacagaacg gccaggtgca ggaatatgac
     1861 atcagcaccg cctcgatgcc atacctcact cgtccaggtc aggttcgcta taagatcatg
     1921 atgggccgtc cgcaagagtg gggacaccat gtcgagggtg aatttttttc tggtgctgaa
     1981 gcttcctggg ggatcgctaa cggctggtcg ttatatggcg gcgcactggg agatgaaaac
     2041 tatcagtctg cggcgcttgg cgtcggtcgc gatttgtcta cattcggcgc ggtcgcgttt
     2101 gatgttactc actcgcacac caaactggat aaagacaccg cttatggcaa aggttcgctg
     2161 gacggtaact ccttccgtgt gagttattcc aaagactttg accagctcaa cagccgcgtt
     2221 acttttgctg gatatcgctt ctcggaagag aactttatga ccatgagcga gtatctggat
     2281 gccagtgaca gcggaatggt acgcacgggc aacgacaaag agatgtacac tgccacttat
     2341 aaccagaact tccgcgatgc gggtgtttcg gtttatctca actatacccg ccatacctac
     2401 tgggatcgcg aggagcagac aaactacaac atcatgctct cgcactattt caatataggc
     2461 agtattcgta atgtcagcat ctcgatgact ggctaccgtt acgagtatga caaccaggcc
     2521 gacaaaggca tgtacatttc gctcagtatg ccgtggggtg acaacagtac cgttagctat
     2581 aacggtaact atggcagtgg gacggacagc agtcaggtcg gttatttcag ccgtgtcgat
     2641 gacgcgactc actatcagtt gaacgtcggc accagtgaca aacacaccag cgttgacggc
     2701 tattacagcc atgatggttc gctggcgcag gttgacctca gtgcgaacta ccatgaaggg
     2761 caatacacct ctgcgggctt gtcgttacag ggcggcgcaa cgcttactgc ccacggtggc
     2821 gcacttcacc gtacccagaa tatgggcggg acacgcttgt tgattgatgc cgatggcgtt
     2881 gccgatgttc cggtagaagg taacggtgct gctgtttata ccaatatgtt tggtaaagcc
     2941 gtcgtttctg acgtcaataa ctattaccgc aatcaggcgt atatcgacct caacaaactg
     3001 ccggaaaacg ccgaagcaac ccagtcggtg gtacaagcca cgctaactga aggggcgatt
     3061 ggctaccgca aatttgccgt catcagtggt caaaaagcga tggcggtgct gcgtttacaa
     3121 gacggcagcc atcctccgtt tggcgcagaa gtaaaaaatg ataacgagca gacagtgggc
     3181 cttgtcgatg atgacggcaa tgtttatctg gctggggtga aacctggcga acacatgagt
     3241 gtgttctgga gtggtgttgc gcattgcgat atcaacctgc cggacccgct gcctgccgat
     3301 ctgtttaacg gcttgttact gccatgccag cataaaggca acgtcgcgcc ggttgttcct
     3361 gatgacataa agcctgtcat tcaggagcag acgcaacagg tgacacccac caatccccct
     3421 gtttctgttt cagctaacca ataacgtgat taaggaatga tccatgtctg atttactttg
     3481 ctctgccaaa ttgggtgcta cgacgctagc gttactgctt agcgctgcca gtttgtcgcc
     3541 caggcttctg taacgccaga tcgtacccgt ttgatattta atgaaagcga caaatcaatc
     3601 agcgtgacgt tgcgcaataa cgatccgaaa ctgccttatc tggcccaaag ctggatcgaa
     3661 gatgaaaagg gtaacaagat cagttcaccg ttaactgttc tgccaccggt gcaacgcatc
     3721 gattcaatga tgaacgggca ggtaaaagtg cagggaatgc cggatatcaa caaattgcct
     3781 gccgatcgtg aaagcctgtt ctacttcaac gtgcgtgaga tcccgccaaa atcaaacaaa
     3841 gccaacacgc tgcaaatagc actacaaacg cgtattaagt tgttctggcg accgaaggcg
     3901 ctggagaacg tcagcatgaa aaacccctgg caatataagg ttactttaac ccgcaacggt
     3961 caggaattta ccgtcaataa cccgacgccg tattacgtca ttttcagtaa tgccagcaca
     4021 cagaaaaatg gcaatcctgc agccggtttc tcgccaatag tgatgtcgcc gaaaacctct
     4081 gcaccgctga atgtgaagat gggtagcgtg ccggtactga cctatgtcaa cgactatggc
     4141 gctcggatgc cgctcttttt cacttgcaac ggtaacagtt gccaggtcga tgaagagcag
     4201 agccgcaaag gctgaggccg ggagagccag tatgaaagga tcttgttctg tctgggcggt
     4261 aactgggtta ctgatatgtt gtgttggtag cggctttgca gttgcagcga gtaagtcagt
     4321 aagtatgaat ttacgggtgc ttgtcgatgc ccctcctcct tgtacggtaa atggtgcggc
     4381 agttgagttt ggtaatgtat tcattaataa gatcaatggg gttgattata aacgtcccat
     4441 tgactactcg cttgtttgta acaatctggc aatggatgac ctgcgtctac agatgcaggc
     4501 gacaacggtc gtgattaacg gtgaaacagt tatcagcacg ggtattcctg ggtttggtat
     4561 tcgggttcag aaaagtagtg atcacacaat actcgactta acctctggct catggttgcc
     4621 atttaatttt tcctcgggag ttcccgttct ggaagctgtg ccggtgaaac agagtggtac
     4681 aacgttagcg gcagctgaat ttaacgccag tgccacaatt gtggtggatt accaatgaaa
     4741 cggatctcgc ttattttgct gtggggcttt tgctcgatgg cactctcaaa cgtctctttt
     4801 cacggttatt tagtgcagcc gcctaactgc acgattagca acgctcaaac cattgaaata
     4861 acttttcagg atgtgcttat tgatgacatt aatggcagca attatgagca aaccgtgccg
     4921 tattccatta cctgcgatac ggcggttcgc gacccattaa tggaaatgac cttgagctgg
     4981 agtggcacac taagcgattt tgataatgct gcagtaagca gcaatatcac tggtttaggc
     5041 attcagctca aacaggcagg gcagagtttt acgatcaata cgccacttat ggtgaatgaa
     5101 accgatttac cggtgttaac ggctgtgcca gtaaaaaaga gtggtgtcgt tctgccagag
     5161 gcggactttg aggcctgggc cacgttacag gtagattacc aatgaagaaa aagcgtacgt
     5221 tgttttttat tagtagcctg atgttgctgg gcagtggaac cacaatagca ggagataatt
     5281 tgcactttac cggcaattta atcagtaaat cgtgtacgcc agtgattaat ggtagccagt
     5341 tggcagaagt acattttcct gccattgccg ccagcgattt aatgaacctt gggcagtcag
     5401 agcgagtgcc attggtgttt caattgaaag attgtcatag ttctacattg tttaatgtca
     5461 aagtaacgtt gacaggaaca gaagatagtg ctctacccgg ctttctggcc tttgactcct
     5521 cgtcatcagc atccggcgct gggattggta ttgaaactgc ggcaggcaca tcggtgccaa
     5581 tcaacaatac aacaggcgtc acgctcccgc tgaatcaggg aaataacagc ctaaatttta
     5641 atacctggtt acaggcaaag agcgggcgag atgtaacgtc cggagacttt agtgccacag
     5701 tgacggcaac ctttgagtat ttctgaaatg aaaaaatgga ccataatttt cgcgagccta
     5761 atgctgctcg ttctgagtgt tgtcggcgca tctaagagtt atgctggaga taaacttatg
     5821 agtgcgagtt ttgacagtgt gagagtttat tatgccatgg acaaggtaac cggtgctgta
     5881 gcgagcagtg ttttcaatgt tacggtgata acgccaaagg aggttacata tgggaaatat
     5941 gattcttttg cgtataaagg cgatacgttg cgggttattt cctggtctgg gtcgggttca
     6001 gctccaactt tggtactgac tgactttgat acaattaata actcaaattg ccctggcata
     6061 gatacgaaaa tttttcgttg tgcatatatg acgtttaaaa ttaccgttgc atctgatgat
     6121 tatggttgcc cttggatcgc atccttttat tcctatacag atttacccgg atttggaagc
     6181 tatacggccc ctacagtgca taacacaata tgcccaacga taccggtcac ttcatacgat
     6241 atctcctgga gtgaaaatta tgtcagccat aataaggcgt tacgaattca gtccacgggg
     6301 tcaacagtga ccaccacgct ttcgacctac ctgatggaag gcggcagact ctgtgatggc
     6361 agtaattttt ctgataatga tggcagagga gcatactgcc gcgcggtatc agaattgtta
     6421 acctttacct cttacggttg tgataaatca acggtaacgg ttacgccaac tcgtcatccg
     6481 gtaacggata aagtactaca cgatattgtg gtgaatgtga ataccagtag tgggcaaccg
     6541 attgattcga cgtgtcgttt tcagtatgta ttgaatgaac tgtagttcgt agtattgctc
     6601 tgatgcgtct tatcaggcct acgaagtgaa ttgcaattta ttgaattatc acatatttgt
     6661 aggccggata aggcgtttac gctgctccgg catttgctgc cagaattcag ggcaactccg
     6721 gcggcaacca ctcttccact tcaatcaaca ccaacaaagc cgcatcaccg ccgtactctt
     6781 tcggtgcctg gtgaaatgcc atcacatgcg gatgttgtgc cagccacagc ggtgtttgtt
     6841 gcttcaaaat atgcttcccg tgcccatgca tcacgcaggc acaaaacaca tgttcgcggc
     6901 gacaggcggc aatcaacgcg cccagttcct gcttggcctg cagctgcgtc agaccgtgta
     6961 aatccaaaaa caattccggt gaataatcgc cgcggcgcag tttcttcgcc tcaaaatggc
     7021 tgacatccgg gcgaacatat ttcaccggac cttcggtatt taataacggc tgaaactcat
     7081 cggagaaata atggctggca tcagcctgct ccgggatcaa gcgtttcacc ggcacttcgc
     7141 tgattttttt acgctgcggt cggtggacaa tcgtgtcctg cttaatcttg cgagtccccg
     7201 ccatcaactg gcgaaacaga gcctggtcct cctcgctaag tgttgttttc tttttcattt
     7261 atctgtctct atttctaatt ttcctacagt ttacccgact cgtagcacaa tcgatcgttt
     7321 taaacgtatt tttcctcact caccgcgcgt gaatgctgat ttatcgtcat cttcatggca
     7381 aactagccgc cgaaattatt gcgatcatgc cctggaggaa tacgtggata aaattttcgt
     7441 tgatgaagca gtaaatgagc tgcaaaccat tcaggacatg ttgcgctggt cggtaagccg
     7501 cttcagcgcg gcaaatatct ggtacggtca cggcaccgat aacccgtggg atgaagccgt
     7561 acagctggtg ttaccatcgc tctacctgcc gctggatatc ccggaagata tgcgcaccgc
     7621 gcgtctgacc tccagcgaaa aacaccgtat tgttgaacgc gtgatccgcc gcgtcaacga
     7681 gcgcattccg gtggcttacc tgaccaacaa agcctggttc tgcggtcatg aattttacgt
     7741 cgatgaacgc gtgctggtgc cgcgctcgcc gattggtgaa ctgatcaaca ataaatttgc
     7801 cggactcatt agcaagcaac cgcagcatat tttagatatg tgtaccggta gcggctgcat
     7861 cgccattgcc tgtgcttatg ccttcccgga agcggaagtc gacgcggtag atatctctcc
     7921 cgacgcgctg gcggttgccg aacagaacat cgaagaacac ggtctgatcc acaacgtcat
     7981 tccgattcgt tccgatctgt tccgtgattt gccgaaagtg cagtacgatc tgattgtcac
     8041 taacccgccg tatgtcgacg cggaagatat gtccgacctg ccaaacgaat accgtcacga
     8101 gccggaactg ggcctggcat ctggcaccga cggccttaaa ctgacgcgtc gcattcttgg
     8161 aaacgcggca gattaccttg ctgatgatgg cgtgttgatt tgtgaagtcg gcaacagcat
     8221 ggtacatctt atggaacaat atccggatgt tccgttcacc tggctggagt ttgataacgg
     8281 cggcgatggt gtgtttatgc tcaccaaaga gcagctgctg gcggcccgtg aatatttcgc
     8341 gatttataaa gattaagtaa acacgcaaac acaacaataa cggagccgtg atggctggaa
     8401 acacaattgg acaactcttt cgcgtaacca ccttcggcga atcgcatggg ctggcgctcg
     8461 gctgcatcgt cgatggtgtt ccgccaggca ttccgctgac ggaagcggac ctgcaacatg
     8521 acctcgaccg tcgtcgccct gggacatcgc gctataccac ccagcgccgc gagccggatc
     8581 aggtcaaaat tctctccggt gtttttgaag gcgttactac tggcaccagc attggcttgt
     8641 tgatcgaaaa cactgaccag cgctctcagg attacagcgc aattaaagac gttttccgcc
     8701 caggccatgc tgattacacc tacgaacaaa aatacggtct gcgcgattat cgcggcggtg
     8761 gacgttcttc cgcccgcgaa accgccatgc gcgtggcggc aggggcgatt gccaaaaaat
     8821 atctcgccga gaaatttggt attgaaatcc gcggctgctt gacccagatg ggcgacattc
     8881 cgctggaaat caaagactgg tcgctggttg aacaaaatcc gttcttctgc ccggacccgg
     8941 acaaaatcga cgcgttagat gaactgatgc gcgcgctgaa aaaagagggc gactccatcg
     9001 gcgcgaaagt caccgttgtt gccagtggtg tccccgccgg acttggcgag ccggtctttg
     9061 accgcctgga tgccgacatc gcccatgcgc tgatgagcat caacgcggtg aaaggcgtgg
     9121 aaattggcga cggttttgac gtagtggcgc tgcgcggcag ccagaaccgc gacgaaatca
     9181 ccaaagacgg tttccagagc aaccatgcgg gcggcattct cggcggtatc agcagcgggc
     9241 agcaaatcat tgctcatatg gcgctgaaac cgacctccag cattaccgtg ccggggcgca
     9301 ctattaaccg ctttggcgaa gaagttgaga tgatcaccaa aggccgtcac gatccctgtg
     9361 tcgggatccg tgcagtgccg atcgctgaag cgatgctggc gatcgtttta atggatcacc
     9421 tgttacggca gcgggcgcaa aatgccgatg tgaagactga tattccacgc tggtaaaaaa
     9481 tgaacaaaac cgcgattgcg ctgctggctc tgcttgccag tagcgctagc ctggcagcga
     9541 cgccgtggca aaaaataacc caacctgtgc cgggtagcgc acaatcgata ggcagttttt
     9601 ctaatggctg tattgtcggc gctgacacgt tgccgataca gtccgaacat tatcaggtca
     9661 tgcgtaccga tcagcgtcgc tatttcggtc acccggatct ggtgatgttt atccagcgtc
     9721 tgagtagcca ggtgagcaat ctgggcatgg gtacggtgct gattggcgat atggggatgc
     9781 ccgctggtgg gcgtttcaac ggcggtcatg ccagccacca gaccggactg gatgtcgata
     9841 tctttctgca actgccgaaa actcgctgga cctccgcgca gctcttgcgc ccgcaagcac
     9901 tggacttagt ttcccgcgac ggtaaacacg ttgtctccac gctgtggaag ccagaaattt
     9961 tcagcttgat caaactcgct gcccaggaca aagacgtcac gcgcattttt gttaatccgg
    10021 cgattaaaca acaactttgc cttgatgcgg gcaccgatcg cgactggttg cgcaaagtgc
    10081 gaccctggtt ccagcatcgc gcgcatatgc atgtacgatt acgttgccct gccgatagtc
    10141 tggagtgtga agatcaacct ttaccgccat caggcgatgg ttgcggggca gaactgcaaa
    10201 gctggtttgc acctctaaaa ccgggaacaa caaagcctga gaagaagaca ccgcctccgt
    10261 tgccgccttc ctgccaggcg ctactggatg agcacgtgat ctaatggaaa cgtttaatag
    10321 cctgtttatg gtttccccgc tgttgctggg agttctcttt tttgtcgcca tgctggcggg
    10381 atttatcgac tcgattgccg gtggcggtgg gttactcacc attccggcat tgatggctgc
    10441 gggaatgtct cccgctaacg cgctggcaac caataaactg caagcctgcg gcggctctat
    10501 ttccgccact atctacttta ttcgccgcaa agtggttagt ttaagcgatc agaaactcaa
    10561 tattgccatg acctttgtcg gctcaatgag cggcgcactg ttggtacagt acgttcaggc
    10621 tgatgtctta cggcagattt tgcccattct ggtgatttgt atcggcctct atttcttgct
    10681 gatgccaaag ctgggtgaag aagatcgcca gcgccggatg tacggtttac ccttcgcgtt
    10741 aattgccggt ggctgcgtcg gtttttatga cggattcttt ggcccggctg ccggatcgtt
    10801 ttacgcgctg gccttcgtta cgctgtgcgg attcaacctc gccaaagcca cggctcacgc
    10861 caaattactc aacgcaacgt caaacattgg cggtctgctg ctgtttattc tcggcggcaa
    10921 agtgatttgg gcgacgggct ttgtcatgct ggtggggcag ttcctcggcg cgcgcatggg
    10981 ttcacggctg gtgttgagca aaggacaaaa gctgatccgc ccgatgattg ttatcgtctc
    11041 ggcggtgatg agtgccaaat tactttacga tagccacgga caggagatcc tccactggtt
    11101 ggggatgaac taatgaacag tacacaccac tatgagcagt tgattgaaat ttttaatagc
    11161 tgctttgccg atgaatttaa tacccgtctg attaaaggcg acgacgaacc gatctatctt
    11221 cctgctgatg cggaagtacc gtataaccga attgtctttg cccacggctt ttatgccagc
    11281 gcaattcatg agatttcgca ctggtgtatt gccggaaaag cgcgccgtga gctggttgat
    11341 ttcggctact ggtattgccc ggatgggcgt gatgcccaaa cgcaaagcca gtttgaagat
    11401 gttgaagtga agccgcaggc actcgactgg ttgttctgtg tggcggcggg atatccgttt
    11461 aacgtcagtt gcgacaatct ggaaggcgac ttcgaaccgg atcgtgttgt gtttcaacgg
    11521 cgggtacatg cgcaggtgat ggattatctg acaaatggca ttcccgagcg tccggcacgc
    11581 ttcattaaag cactacaaaa ttattatcac acgccggaac ttacggcgga acagttcccg
    11641 tggccggaag cgctcaactg aagccggtgc cacgttttac aatgaggaaa gaagatgatc
    11701 gcggagtttg aatcacgcat tctggcatta atcgacggca tggttgacca tgccagtgat
    11761 gatgagttgt ttgccagtgg gtatttgcgt ggccacctga cattagccat cgcagaactg
    11821 gaaagtggtg atgaccactc cgctcaggcg gtgcatacga ccgttagcca gagtctggaa
    11881 aaagccattg gtgccggtga attgtcgccg cgtgaccagg cgctggtgac cgatatgtgg
    11941 gaaaacctgt ttcagcaggc gtcacagcag taagttgcgg cgtctggagc accatgcctg
    12001 atgcgacgct tatcgcgtct tatcaggcct acacgtcaca cgtaggtcgg ataaggcgtt
    12061 cacgccgcat ccggcaaaca ctgcgagagc agattacccc gccttaaccg ctttaccctt
    12121 caacaatttc cgcacccata accgattcgg gtttaacgcc gtcagcgtac tggcatccat
    12181 cggaatcggt tcgtcgctca tctgcgccgc cagaatctcg gcacacagcg gggcagaaca
    12241 caaaccgcga gaacctaaag ccgcaaacat aaagagatcg tcaaaaaccg gcgcgcttac
    12301 cgcctcatct ttctgctccg ccaacgacgc atattccacg agtgttgcct cataatcggg
    12361 aacattgcct accattggca gatgatcgcg ggtggcacaa cgcacaccgc agcgcgcctc
    12421 tttatcactg acatcaacct cttttgccca ctgtgcctgc gggaaacaat caatcaaccg
    12481 ctggcgattc tgctgctgat cgtcctcact gtacgccgta tcttcgctgc cgcgatgata
    12541 actggcacca atacaatgat gttgattcgc cggattttgt ggcgtgagat aaccgtcata
    12601 gcacagcacc tgcttcagct ctgccagttc cggcgttgtc ggaatatggc tgacctgccc
    12661 ggcaaccgaa tacaccggga gagtcgacgt ttggctgaaa cggctgattt gatgcccgtt
    12721 cgccagtacc actacgctgt gtgttgcttg ctgatctcct gcaaaattca acaaccaaca
    12781 gtcatcctta cgggataaat tctgtaactg atattgataa taaatctgca aaccctgctg
    12841 ttgcgccagt tccagcacat tacgggtcag ttctgctggg cacagccaac caccttgcgg
    12901 ataagtaatg ccgctgcaat ttgtcgcaac gcccgtaatt tgttcaaccg catttgcctc
    12961 aacggctaca gccagttctg cgggtaaatc cattgacaac atctgtgcga ttttatgctg
    13021 gcttttctca tcccagccta actgcgtgac gccgcaccag tcatgatcaa atttaacggg
    13081 taattggtcg taaaaccgac gagcaaaagt aaacgcatta gagaaaaagc ggtttagcgc
    13141 ctcatcgtgt ttgcttaata acggatacag cgccccctgg cgattgccgg aagcacccag
    13201 cgcgggggct tcatccgcgc aataaagcgc tacctgccag ccgcgccgta ataacgccag
    13261 cgacaacaac gcgctggcaa taccaccgcc gataatcgct acttcccgtt tgctgctgcc
    13321 cgtacgatta aaccacggcg tggagcaggg gagtggtaat gtctgttcca tcaccccaca
    13381 aagcatttcc cgtttgcgcc caaagccctt acgtttttgc atcgtgaatc cggcttcctg
    13441 caaaccgcgg cggacaaaac cggcagacgt aaatgtcgcc agcgtgccgc ctggacgcgc
    13501 caaccttgcc atggcgttaa acagattttg cgtccacata tccgggtttt tagctggcgc
    13561 aaagccgtcc agaaaccagg catctacttt ttgatttagc gaatcgtcca gttggctggt
    13621 cagttcgtta atatcgccaa accataaatc cagcgtcacg cggccttcat tgagcaataa
    13681 acgatggcaa ccgggcaagg gcattggcca ctgcgcctga agttgttctg cccacggagc
    13741 cagttccggc cagtgttgat gcgctaaggc taaatccgca cgggtgaggg gaaatttctc
    13801 aaaactaatg aaatgtaagc gttgtaattg cgcttgcgga tgcgcttcgc gaaactgatc
    13861 aaatgcctgc catagcgtca ggaagtttaa tccggtgccg aagccgctct ctgctaccac
    13921 aaacagagga tgtggatgct caggaaagcg tacctctaat tggttgcctc ccagaaaaac
    13981 ataacgcgtc tcttccagcc cgttatcgtt ggaaaaatag acatcgtcaa aatctcggga
    14041 aacaggtgta ccctcagcat taaattcgag gttggcaggt tgtatggagt agtgtttcac
    14101 gtaagttact cgtcttacag gcggtggctc gatcttagcg atgtgtgtaa ggctgcgcaa
    14161 atttctctat taaatggctg atcggacttg ttcggcgtac aagtgtacgc tattgtgcat
    14221 tcgaaactta ctctatgtgc gacttacaga ggtattgaat gaaacgtgca gtgattactg
    14281 gcctgggcat tgtttccagc atcggtaata accagcagga agtcctggca tctctgcgtg
    14341 aaggacgttc agggatcact ttctctcagg agctgaagga ttccggcatg cgtagccacg
    14401 tctggggcaa cgtaaaactg gataccactg gcctcattga ccgcaaagtt gtgcgcttta
    14461 tgagcgacgc atccatttat gcattccttt ctatggagca ggcaatcgca gatgcgggcc
    14521 tttctccgga agcttaccag aataacccgc gcgttggcct gattgcaggt tctggcggcg
    14581 gttccccgcg tttccaggta ttcggcgctg acgcaatgcg cggcccgcgc ggcctgaaag
    14641 cggttggccc gtatgtggtc accaaagcga tggcatccgg cgtttctgcc tgcctcgcca
    14701 cgccgtttaa aattcatggc gttaactact ccatcagctc cgcgtgtgcg acttccgcac
    14761 actgtatcgg taacgcagta gagcagatcc aactgggcaa acaggacatc gtgtttgctg
    14821 gcggcggcga agagctgtgc tgggaaatgg cctgcgaatt cgacgcgatg ggtgcgctgt
    14881 ctactaaata caacgacacc ccggaaaaag cctcccgtac ttacgacgct caccgtgacg
    14941 gtttcgttat cgctggcggc ggcggtatgg tagtggttga agagctggaa cacgcgctgg
    15001 cgcgtggtgc tcacatctat gctgaaatcg ttggctacgg cgcaacctct gatggtgcag
    15061 acatggttgc tccgtctggc gaaggcgcag tacgttgtat gcagatggcg atgcatggcg
    15121 ttgatacccc aatcgattac ctgaactctc acggtacttc gactccggtt ggcgacgtga
    15181 aagagctggc agctatccgt gaagtgttcg gcgataagag cccggcgatt tctgcaacca
    15241 aagccatgac cggtcactca ctgggcgctg cgggtgtgca ggaagctatc tactctctgc
    15301 tgatgctgga acacggcttt atcgccccga gcatcaacat tgaagagctg gacgagcagg
    15361 ctgcgggtct gaacatcgtg accgaaacga ccgatcgcga actgaccact gttatgtcta
    15421 acagcttcgg cttcggcggc accaacgcca cgctggtaat gcgcaagctg aaagattaat
    15481 tcgcagtagg ccggagtagg tcggataaga cgcgccagcg tcgcatccga cgttacgcgc
    15541 caatgcggcc tccggcacta acgcaaaagg gaacctgatg gttccctttt tcacatcatt
    15601 gacaatcgcc accagttcca ggcaaacttc ccgctttgtc gatttccttc tgaaaagacg
    15661 tacgcgttaa atcctgccaa cgcaccgtaa ccctgaaacc agagagatga gacggggata
    15721 ctcctcgcct tgcgctgcat tctggagtaa tgcatgactg ctgtaagcca aaccgaaaca
    15781 cgatcttctg ccaatttttc gctcttccgc atcgcttttg cggtttttct cacctacatg
    15841 accgtagggc tgccgttgcc ggttatcccg ctgtttgttc atcatgaact gggctatggc
    15901 aataccatgg tcggcattgc cgtcgggatt cagtttctgg ctacggtgct gacgcgtggc
    15961 tatgccgggc gactggccga tcaatatggc gcaaaacgtt cggcgcttca ggggatgtta
    16021 gcttgtggtc tggctggcgg cgcgttgctg ctggcggcga ttttgcctgt ctccgcaccg
    16081 ttcaaatttg ccctgttggt catcgggcgt ttgattcttg ggtttggtga aagccagtta
    16141 ctgacaggcg ctctgacctg ggggttaggc atcgtagggc caaaacactc tggcaaagtg
    16201 atgtcatgga atggaatggc gatttacggt gccctcgctg ttggtgctcc gcttggcctg
    16261 ttgattcata gccattacgg ttttgccgca ctggcgatca ccacaatggt attaccctta
    16321 ctggcgtggg cctgtaacgg cacagtgcgc aaagtaccgg ccctggcggg agaacgtcca
    16381 tcgctgtgga gcgttgtcgg gtttatctgg aaaccagggt tagggctggc actacaaggc
    16441 gttggttttg cggttatcgg gactttcgtt tcgctctact ttgccagcaa aggatgggcg
    16501 atggcgggct ttactcttac cgagtttggc ggcgcatttg tcgtgatgcg cgtcatgttt
    16561 ggctggatgc cggaccgttt tggcggcgtg aaagtggcga ttgtctctct gcttgtagaa
    16621 acggtgggct tgttgctgct ctggcaagcc ccaggtgcgt gggtcgcatt agcgggcgcg
    16681 gcgttaaccg gagccggatg ttcgcttatc tttcctgcgc tgggcgtgga ggtggttaaa
    16741 cgcgtcccct cacaagttcg cggcaccgca ctgggcggtt acgccgcgtt tcaggatatc
    16801 gccctcggcg tctccgggcc gctggcggga atgctggcga ccacgtttgg ttactcttcg
    16861 gtatttcttg ccggggcgat ctctgcggtg ctgggaatta ttgtcacgat actgtcattt
    16921 cgtcggggct aacgaaccag ccagaccagc atcaggataa tcgcgactaa cagtatccac
    16981 agcgcgggac gcgttgccat attttgcaac gtgtccatca tcggtggaaa aggattaagc
    17041 agcggttgca tatcacgcgg atcaatccct ttaacccgcc gttgataaag ctgattaaga
    17101 atatcctcgg cctgggcaga agaaagcgat gattgcgcag aaagaccata tttttgctgg
    17161 atatacgccg ataacgctgc cagttcactg gcatctaaag gttgttttag cgccatctgt
    17221 agtgattcca gcgtcggcgt attttgctgg cttaacgtct ggcgcgcctg caaccaggtc
    17281 accagatggt taaacagttt cgctggaatt aactcgccat cgttcacccc ggaaagttcc
    17341 agcatcgatt gccagatctg tttgctgggt tcccccgttg ccgccgcaag tttggtcacc
    17401 agctgtttta gcgcattgtg ctccgccggt aataaaggac ggtcggtcgc ctcgcgctgc
    17461 tgtggttgcg gaataaccat cttcccttcc tgcaacaggg tgagaatggt ttttaattgc
    17521 tccggtgaga gctgattcag cggcgtctga ccaaagttat gacggatata atccgtgacc
    17581 gcctgacgat tattccccag acgtaaatac tcccctaatt gcgctaaaag ctggcgggca
    17641 gaatggcttt tttgcgcggc cagcagacgt tgcgccagat tatgctcagc ggcagggaag
    17701 tgacgcgaaa gcagcggtga atctcccgac agaccaatat cgtgcctgat acccgcccac
    17761 agttctgctc tttgttgttg cgtcagtgag gtcactttcg tcattaagct ttccagcgaa
    17821 gtacgttgct gactggataa aggctgattg cccgcgccag acggcaggtt atctccctga
    17881 cctggtggtt gcccaggagg agggccggaa ataggttgta tcattacgta tccttatacc
    17941 tgaaatcttc gcaagtatgc ctggccgcga gattatggca cacttgtccg gttaactctc
    18001 gtctcataca ggtaacacaa acgtgaaaat ccttgttgat gaaaatatgc cttatgcccg
    18061 cgacttattt agccgtttgg gtgaggtgac cgcggttccc gggcgtccaa tccccgtcgc
    18121 tcaactggca gacgcggatg cgctgatggt gcgttcggtc acgaaagtga atgaatcttt
    18181 gctggcagga aaacccatta aatttgttgg cactgccact gcggggaccg accatgtcga
    18241 tgaagcatgg ttgaagcagg cgggaattgg tttttccgct gcacctggct gtaatgcgat
    18301 tgcggtggtg gaatatgttt tctcctcact actgatgctt gccgaacgcg atggattttc
    18361 actgcacgac cgtacggtgg ggatcgtggg cgttggtaac gttggacgtc gattgcaggc
    18421 gcgactggaa gcgttaggga ttaaaacatt gctttgcgat ccgcctcgcg ctgaccgtgg
    18481 ggatgagggt gatttccgct cgctggatga attagttcag catgcggata ttctgacttt
    18541 ccatacgcca ctcttcaaag atgggccgta caaaacgctg catctggcgg atgaaaaact
    18601 gatccgtagc ctgaagccgg gagcgattct gattaacgcc tgccgtggcg cagtcgtcga
    18661 taatactgcg ttgctgacct gcctgaatga aggccagaag ttaagcgtag tgctggatgt
    18721 ctgggaaggc gaaccggaac ttaacgtgga gttgctgaca aaagtggata tcggcacgcc
    18781 gcatatcgca ggttataccc tggaaggtaa agcacgcggt actacgcaag tgtttgaagc
    18841 ctatagcaag tttattgggc atgaacagca cgttgcgctg gatacattac tgcccgcgcc
    18901 agagtttggt cgcattacgc tgcatggccc gctcgatcaa ccgacgctga aaaggctggt
    18961 gcatttggtg tatgatgtgc gccgcgatga cgcaccgctg cgtaaagtcg ccgggatacc
    19021 gggtgagttc gataaactgc gcaaaaacta tcttgagcgc cgtgaatggt catctctgta
    19081 tgtaatttgt gatgacgcca gtgcggcatc attgctgtgt aaactgggtt ttaacgccgt
    19141 tcatcatccg gcacgttaat ctcttcttca tgctctctgc tgtaacattg gcagggagct
    19201 ttgctatttc tggagtaaac caccatgtct gaaggctgga acattgccgt cctgggcgca
    19261 actggcgctg tgggcgaagc cctgcttgaa acgctggctg aacgtcagtt cccggttggg
    19321 gaaatttatg cactggcacg taacgaaagc gcaggcgaac aactgcgctt tggtggtaaa
    19381 acaatcactg tgcaggatgc cgctgaattc gactggacgc aggcgcagct ggcatttttt
    19441 gtcgcaggca aagaagctac cgctgcctgg gttgaagaag cgaccaactc aggttgcctg
    19501 gtgatcgaca gcagcggact gtttgctctc gaacccgacg taccgctggt ggtgccggaa
    19561 gtaaacccgt ttgtactgac cgattaccgc aaccggaatg tcatcgccgt accagacagt
    19621 ctgaccagcc agctgctggc ggcactgaaa ccgttaatcg atcagggcgg tttgtcgcgt
    19681 atcagtgtta ccagcctgat ttcagcctcc gcccagggca aaaaagcggt cgatgcgtta
    19741 gcggggcaga gtgcgaaatt gctcaacggc attccgattg acgaagaaga tttcttcggt
    19801 cgtcaactgg cgttcaatat gctgccgttg ttgccggata gcgaaggtag cgtgcgtgaa
    19861 gaacgtcgta tcgttgacga agtacgcaaa atcctgcagg acgaagggct gatgatttcg
    19921 gcaagcgtcg tccaggcacc ggtattctac ggtcatgccc agatggtcaa ctttgaagca
    19981 ctgcgtccgc tggcggcaga agaagcgcgt gatgcgtttg ctcagggcga agatattgtg
    20041 ctctctgaag agaacgaatt cccgactcag gtaggggatg cttcgggtac gccgcatctt
    20101 tctgttggct gcgtgcgtaa tgactacggt atgccggagc aagtccagtt ctggtcggtg
    20161 gccgataacg ttcgctttgg cggcgcgctg atggcagtaa aaatcgccga gaaactggtg
    20221 caggagtatc tgtactaatg tccgaccagc aacaactgcc agtttataaa attgcgctgg
    20281 gcattgagta cgacggcagt aagtattacg gctggcaacg gcagaatgaa gtccgcagtg
    20341 tgcaggagaa gctggaaaag gcgctctccc aggtggcgaa cgaacccatc accgtcttct
    20401 gcgccgggcg tactgacgca ggggtacacg gtaccgggca ggtggtgcat ttcgaaacaa
    20461 ccgcgcagcg caaagacgcg gcgtggacct tgggcgtaaa tgcgaattta cctggtgaca
    20521 tcgccgtgcg ttgggttaaa gctgtacctg atgattttca tgcccgattt agcgccacgg
    20581 ctcgccgtta tcgctacatc atctacaatc atcggctgcg cccggcggta ttgagtaaag
    20641 gggtaaccca tttttacgaa ccgctggacg ctgaacggat gcatcgggct gcgcaatgct
    20701 tgctgggcga gaatgatttc acctcgttcc gtgcggtgca gtgccagtcc cgaaccccgt
    20761 ggcgcaacgt tatgcatatt aatgtcacgc gtcacggtcc ttatgtggtg gtagatatca
    20821 aagcgaatgc ctttgtacat catatggtca ggaatattgt cggcagcctg atggaagtcg
    20881 gtgcccacaa ccagccggag agatggatag cagagttgct ggcggcaaag gacagaacgc
    20941 tggcggcagc aacggcaaaa gcggaagggc tgtatctggt cgcggtggat taccctgacc
    21001 ggtatgatct tccaaaaccg ccaatgggcc cgctatttct ggcggactaa cgagaataat
    21061 gctcggaaca tttcaggctg tagccaacgt agttacagcc tgaaagatga cgagtacaag
    21121 gcataggcaa atatggacct gatttatttc ctcatcgatt ttatcctgca cattgacgtg
    21181 catctggcag agctggtcgc ggagtacggc gtctgggttt atgccattct gtttttgatt
    21241 ctgttctgcg aaacggggct ggtggtaacg ccattcctgc ctggtgattc gctgctgttt
    21301 gttgctggtg cattggcgtc gctggaaact aacgatctca atgtccatat gatggtggtg
    21361 cttatgttga ttgccgctat tgtcggtgat gcagtgaact ataccattgg acggttattt
    21421 ggtgacaaac tgttcagtaa cccggattcg aaaatctttc gtcgcagtta tcttgataag
    21481 actcatcagt tttacgagag acatggcggc aaaacgatta ttctcgcccg ttttgtaccg
    21541 atcgtcagaa cgttcgcgcc gtttgttgcg ggaatgggcc acatgtcgta ccgtcatttc
    21601 gccgcttata acgtgatcgg cgcactgttg tgggtactga tttttaccta cgcgggttat
    21661 ttcttcggca cgctcccctt cattcagagc aaccttaagc tgatgattgt tggcattatt
    21721 tttgtttcaa tattgcctgg ggttttcgaa tttattcgtc acaaacgcgc tgcggcacgc
    21781 gccgcaaaat aaaaagtaac tccgcggttc gaccactttt ttatccaaag tttcgggctg
    21841 ttatgtttta atgtgcaaca ttcatggtct gttgggggca aaaatggcat tatgcgtccc
    21901 caaagataaa actggcatcg aaccaggttc agacagaaag gtccctaatg agctggattg
    21961 aacgaattaa aagcaacatt actcccaccc gcaaggcgag cattcctgaa ggggtgtgga
    22021 ctaagtgtga tagctgcggt caggttttat accgcgctga gctggaacgt aatcttgagg
    22081 tctgtccgaa gtgtgaccat cacatgcgta tgacagcgcg taatcgcctg catagcctgt
    22141 tagatgaagg aagccttgtg gagctgggta gcgagcttga gccgaaagat gtgctgaagt
    22201 ttcgtgactc caagaagtat aaagaccgtc tggcttctgc gcagaaagaa accggcgaaa
    22261 aagatgcgct ggtggtgatg aaaggcactc tgtatggaat gccggttgtc gctgcggcat
    22321 tcgagttcgc ctttatgggc ggttcaatgg ggtctgttgt gggtgcacgt ttcgtgcgtg
    22381 ccgttgagca ggcgctggaa gataactgcc cgctgatctg cttctccgcc tctggtggcg
    22441 cacgtatgca ggaagcactg atgtcgctga tgcagatggc gaaaacctct gcggcactgg
    22501 caaaaatgca ggagcgcggc ttgccgtaca tctccgtgct gaccgacccg acgatgggcg
    22561 gtgtttctgc aagtttcgcc atgctgggcg atctcaacat cgctgaaccg aaagcgttaa
    22621 ttggctttgc cggtccgcgt gttatcgaac agaccgttcg cgaaaaactg ccgccaggat
    22681 tccagcgcag tgaattcctg atcgagaaag gcgccatcga catgatcgtc catcgtccgg
    22741 aaatgcgcct gaaactggcg agcattctgg cgaagttgat gaatctgcca gcgccgaatc
    22801 ctgaagcgcc gcgtgaaggc gtagtggtac ccccggtacc ggatcaggaa cctgaggcct
    22861 gataactgat aagggcaggg ccactggctc tgcccttttg ctattctcac cgtaacgaat
    22921 cagcggatac catgattatc aaacgcactc ctcaagccgc gtcgcctctg gcttcgtggc
    22981 tttcttatct ggaaaacctg cacagtaaaa ctatcgatct cggccttgag cgcgtgagcc
    23041 aggtcgcggc gcgtcttggc gtcctgaaac cagcgccatt tgtgtttacc gttgcgggta
    23101 cgaatggcaa aggcaccacc tgccgtacgc tggagtcgat tctgatggcg gcagggtaca
    23161 aagtgggcgt ctacagttca ccgcatctgg tgcgttatac cgagcgcgta cgtgtgcagg
    23221 ggcaggaatt gccggaatcg gcccacaccg cctcttttgc ggagattgaa tcggcacgcg
    23281 gtgatatttc cctgacctat ttcgagtacg gtacgctgtc ggcgttgtgg ctgttcaaac
    23341 aggcacaact tgacgtagtg attctggaag tagggctggg cggtcgtctg gacgcaacca
    23401 atattgtcga tgccgatgtg gctgtagtaa ccagcattgc gctggatcat accgactggc
    23461 tgggaccaga tcgcgaaagt attggtcgcg agaaagcagg catctttcgc agcgcaaaac
    23521 cggcaattgt cggtgagccg gaaatgcctt ctaccattgc tgatgtggcg caggaaaaag
    23581 gtgcactgtt acaacgtcgg ggcgttgagt ggaactattc cgtcaccgat catgactggg
    23641 cgtttagcga tgctcacggc acgctggaaa atctgccgtt gccgcttgtc ccgcaaccga
    23701 atgccgcaac agcgctggcg gcactgcgtg ccagcgggct ggaagtcagt gaaaatgcca
    23761 ttcgcgacgg gattgccagc gcaattttgc cgggacgttt ccagattgtg agcgagtcgc
    23821 cacgcgttat ttttgatgtc gcgcataatc cacatgcggc ggaatatctc accgggcgta
    23881 tgaaagcgct accgaaaaac gggcgcgtgc tggcggttat cggtatgcta catgataaag
    23941 atattgccgg aactctggcc tggttgaaaa gcgtggttga tgactggtat tgtgcgccac
    24001 tggaagggcc gcgcggtgcc acggcagaac aactgcttga gcatttgggt aacggcaaat
    24061 catttgatag cgttgcgcag gcatgggatg ccgcaatggc ggacgctaaa gcggaagaca
    24121 ccgtgctggt gtgtggttct ttccacacgg tcgcacatgt catggaagtg attgacgcga
    24181 ggagaagcgg tggcaagtaa gtttcagaat cggttagtgg gcacgatcgt gctggtggcg
    24241 ctgggggtga ttgtacttcc agggctgctg gacgggcaga aaaaacatta tcaggatgag
    24301 tttgcggcta tcccgctggt tccaaaagcg ggcgatcgcg atgagcctga tatgatgcca
    24361 gccgccaccc aggcgttgcc gacgcagccg ccggaaggcg cagcggaaga ggtgcgggca
    24421 ggtgatgccg cagcaccgtc gctcgatccg gccactattg cagccaataa caccgagttt
    24481 gaaccggaac ctgcaccggt cgccccaccg aagccgaaac cggtggagcc gcctaaaccg
    24541 aaggttgaag caccacctgc gccgaagcca gaaccgaagc cggtcgtgga agaaaaagca
    24601 gcgccaacgg gtaaagccta tgttgtgcaa ctgggtgcgc tgaaaaatgc cgataaagtg
    24661 aatgagattg tcggtaagct gcgcggtgcc ggttatcggg tttatacgtc gccatccacg
    24721 ccagtgcagg gtaaaattac ccgtattctg gttgggccgg atgcctcgaa agataagctg
    24781 aaaggttcgc tgggtgagtt gaagcaactt tctggcttaa gtggcgtggt aatgggctat
    24841 acgccgaatt aatacagtct tgcctgatgc gacgctggcg cgtcttatca ggcctgggca
    24901 gtgttagaac cgtaggtcgg ataaggcgtt tacgccgcat ccgacacgca ttgcacgatg
    24961 ccgcaaaggc ataaaaagtc gatggcgttg aatatttttt cagcgccatt tttattgatg
    25021 cgtgggaagg aaatccctac gcaaacgttt tctttttctg ttagaatgcg ccccgaacag
    25081 gatgacaggg cgtaaaatcg tgggacacat atggtctgga ttgattacgc cataatcgcg
    25141 gtgattgctt tttcctctct ggttagcctg atccgcggct ttgttcgtga agcgttatcg
    25201 ctggtgacat ggggttgtgc tttctttgtt gccagtcatt actacactta cctgtcagtc
    25261 tggtttacgg gctttgaaga cgaactggtt cgaaatggaa ttgccatcgc ggtactgttt
    25321 atcgctaccc tgattgttgg tgctatcgtg aacttcgtga taggccagtt ggtggagaaa
    25381 acggggttgt caggcaccga tcgggtgctg ggcgtctgtt tcggtgcgtt gcgcggtgtg
    25441 ttgattgttg ccgccattct cttctttctc gactccttta ccggggtgtc gaaaagcgaa
    25501 gactggagca aatcacagct gatcccgcag ttcagtttta tcatcagatg gttttttgat
    25561 tatctgcaaa gctcgtcaag tttcttgccc agagcgtaag tgctctgaga tgtggcttaa
    25621 cgaggaaaaa gacgtatgtg cggtattgtc ggtatcgccg gtgttatgcc ggttaaccag
    25681 tcgatttatg atgccttaac ggtgcttcag catcgcggtc aggatgccgc cggcatcatc
    25741 accatagatg ccaataactg cttccgtttg cgtaaagcga acgggctggt gagcgatgta
    25801 tttgaagctc gccatatgca gcgtttgcag ggcaatatgg gcattggtca tgtgcgttac
    25861 cctacggctg gcagctccag cgcctctgaa gcgcagccgt tttacgttaa ctccccgtat
    25921 ggcattacgc ttgcccacaa cggcaatctg accaacgctc acgagttgcg taaaaaactg
    25981 tttgaagaaa aacgccgcca catcaacacc acttccgact cggaaattct gcttaatatc
    26041 ttcgctagcg agctggacaa cttccgccac tacccgctgg aagccgacaa tattttcgct
    26101 gccattgctg ccacaaaccg cttaatccgc ggcgcatatg cctgtgtggc gatgattatc
    26161 ggccacggta tggttgcttt ccgcgatcca aacgggattc gtccgctggt actgggaaaa
    26221 cgtgatattg acgagaaccg tacagaatat atggtcgctt ccgaaagcgt agcgctcgat
    26281 acgctgggct ttgatttcct gcgtgacgtc gcgccgggcg aagcgattta catcactgaa
    26341 gaagggcagt tgtttacccg tcaatgtgct gacaatccgg tcagcaatcc gtgcctgttt
    26401 gagtatgtat actttgctcg cccggactcg ttcatcgaca aaatttccgt ttacagcgcg
    26461 cgtgtgaata tgggcacgaa actgggcgag aaaattgccc gcgaatggga agatctggat
    26521 atcgacgtgg tgatcccgat cccagaaacc tcgtgtgata tcgcgctgga aattgctcgt
    26581 attctgggca aaccgtaccg ccagggcttc gttaaaaacc gctatgttgg ccgcaccttt
    26641 atcatgccgg gccagcagct gcgtcgtaag tccgtgcgcc gtaaactgaa tgccaaccgc
    26701 gccgagttcc gcgataaaaa cgtcctgctg gtcgacgact ccatcgtccg tggcaccact
    26761 tctgagcaga ttatcgagat ggcacgcgaa gccggagcga agaaagtgta cctcgcttct
    26821 gcggcaccgg aaattcgctt cccgaacgtt tatggtattg atatgccgag cgccacggaa
    26881 ctgatcgctc acggtcgcga agttgatgaa attcgccaga tcatcggtgc tgacgggttg
    26941 attttccagg atctgaacga tctgatcgac gccgttcgcg ctgaaaatcc ggatatccag
    27001 cagtttgaat gctcggtgtt caacggcgtc tacgtcacca aagatgttga tcagggctac
    27061 ctcgatttcc tcgatacgtt acgtaatgat gacgccaaag cagtgcaacg tcagaacgaa
    27121 gtggaaaatc tcgaaatgca taacgaagga tgatgccttc gctgagggtg ccggtctggc
    27181 accctgactt gcaactcccg ccgaaatact gcaaagtctg cctgcaagtc tgacagggca
    27241 actatttatg aaacgactca ttgtaggcat cagcggtgcc agcggcgcga tttatggcgt
    27301 gcgcttatta caggttctgc gcgatgtcac agatatcgaa acgcatctgg tgatgagcca
    27361 ggcggcgcgc cagaccttat ccctcgaaac ggatttttcc ctgcgcgaag tgcaggcatt
    27421 agctgatgtc acgcacgatg cgcgcgatat ttccgccagc atctcttccg gttctttcca
    27481 gacgctgggg atggtgattt taccctgttc aatcaaaacc ctttccggca ttgtccatag
    27541 ctatactgat ggcttactga cccgtgcggc agatgtggtg ctgaaagagc gtcgcccgtt
    27601 ggtgctctgc gtgcgtgaaa caccattgca cttaggccat ctgcgtttaa tgactcaggc
    27661 ggcagaaatc ggtgcggtga ttatgcctcc cgttccggcg ttttatcatc gcccgcaatc
    27721 ccttgatgat gtgataaatc agacggttaa tcgtgttctt gaccagtttg caataaccct
    27781 tcctgaagat ctctttgccc gctggcaggg cgcataactc cgctgttgcc ctgtttcagg
    27841 gcaattttgc aaccgcgatc aaatcctcga cattttgttt ctgccattca atcgaaacgc
    27901 tgcgattcaa ccgctatacc tgctatcttc aacttcagga caataatgca acgtcttatt
    27961 aacatattta acgttgaatg ttactgttgt cgtcaagatg gcataagacc tgcatgaaag
    28021 agcctgcaaa cacacaacac aatacacaac ataaaaaagc cattttcact tgagggttat
    28081 gtatgaagaa gtcgattctc gctctgtctt tgttagtcgg tctctccgca gcggcttcca
    28141 gctatgcggc gctaccggag acggtacgta tcggaaccga taccacctat gcgccgttct
    28201 cgtcgaaaga tgctaaaggt gattttgttg gctttgatat cgatctcggt aacgagatgt
    28261 gcaaacggat gcaggtgaaa tgtacctggg ttgccagtga ctttgacgcg ctgatcccct
    28321 cactgaaagc gaaaaaaatc gacgccatta tttcgtcgct ttccattacc gataaacgtc
    28381 agcaggagat tgccttctcc gacaagctgt acgccgcaga ttctcgtttg attgctgcca
    28441 aaggttcacc gattcagcca acgctggatt cactgaaagg taaacatgtt ggtgtgctgc
    28501 agggatcaac ccaggaagct tacgctaacg agacctggcg tagcaaaggc gtggatgtgg
    28561 tggcctatgc caaccaggat ttggtctatt ccgatctggc tgcaggacgt ctggatgctg
    28621 cgttacaaga tgaagttgct gccagcgaag gattcctcaa gcaacctgct ggtaaagctt
    28681 tcgcctttgc tggctcatca gtaaaagaca aaaaatactt cggtgatggc accggtgtag
    28741 ggctacgtaa agatgatgct gaactgacgg ctgccttcaa taaggcgctt ggcgagctgc
    28801 gtcaggacgg cacctacgac aagatggcga aaaagtattt cgactttaat gtctacggtg
    28861 actgatacgt cgctgggaag ctgtacctga tggaatgatc atcatggtgc acgccaggtt
    28921 tgttgcacta tcgtggtgca ttgaaatgca tacttaagca tttttaatga aaaataatac
    28981 gtctaacggg gcggaatatt ttgccttgat ggtcaatttt atggcacgat aagtgtaaca
    29041 aacctgtaaa tattccctat aaaaagactg tcagttgagg acattatgaa aaaactggtg
    29101 ctatcgctct ctctggttct ggccttctcc agcgcaactg cggcatttgc tgcgattccg
    29161 caaaacatcc gcatcggtac cgacccgacc tatgcgccat ttgaatcaaa aaattcacaa
    29221 ggcgaactgg ttggcttcga tatcgatctg gcaaaggaat tatgcaaacg catcaatacg
    29281 caatgtacgt ttgtcgaaaa tccgctggat gcgttaatcc cgtccttaaa agcgaagaag
    29341 attgacgcca tcatgtcatc gctttccatt acggaaaaac gtcagcaaga aatagccttc
    29401 accgacaaac tgtacgctgc cgattctcgt ttggtggtgg cgaaaaattc tgacattcag
    29461 ccgacagtcg agtcgctgaa aggcaaacgg gtaggcgtat tgcagggcac cacccaggag
    29521 acgttcggta atgaacattg ggcaccaaaa ggcattgaaa tcgtctcgta tcaggggcag
    29581 gacaacattt attctgacct gactgccgga cgtattgatg ccgcgttcca ggatgaggtc
    29641 gctgccagcg aaggtttcct caaacaacct gtcggtaaag attacaaatt cggtggcccg
    29701 tctgttaaag atgaaaaact gtttggcgta gggaccggca tgggcctgcg taaagaagat
    29761 aacgaactgc gcgaagcact gaacaaagcc tttgccgaaa tgcgcgctga cggtacttac
    29821 gagaaattag cgaaaaagta cttcgatttt gatgtttatg gtggctaatc gccatcggtg
    29881 aaagaaagcc cgctcccttc gggtaaccgg agggagacaa gaggcggtaa ttcaccacac
    29941 acgacaggac aggcagcatg ttgtatgggt tttcaggtgt tattttacag ggtgcgctcg
    30001 tcacgctgga gctggctatc agctctgtag tgctcgctgt aatcatcggt ttaattggcg
    30061 ctggcggtaa gctctcgcaa aatcggcttt cggggctgat tttcgaaggg tacaccacgc
    30121 tgattcgtgg cgtgccggac ttagtgttga tgctgctgat tttctacggt ttgcagattg
    30181 cgctaaacac ggtgacggag gcgatgggcg tcgggcagat tgatatcgat ccgatggtcg
    30241 ctggtattat cactctcggt tttatctacg gtgcttattt taccgaaacg ttccgtggtg
    30301 cttttatggc agtgccgaaa ggacatatag aggcggcgac ggcgttcggt tttactcgtg
    30361 ggcaagtgtt tcggcggatc atgtttccgg cgatgatgcg ttacgccctg ccaggcattg
    30421 gcaacaactg gcaggtgatc ctcaaatcta ccgcactggt ttcgttactc ggcctggaag
    30481 atgtggtcaa agccacccaa ctggcaggca aaagtacctg ggaaccgttc tatttcgcca
    30541 tcgtctgtgg cgtgatttac ctggttttca ccactgtttc caatggtgtg ctgctgttcc
    30601 ttgagcgccg ctactccgtg ggtgtgaaga gggctgacct gtgatcgaaa tcttacatga
    30661 atactggaaa ccgctgctgt ggaccgacgg ttatcgcttt actggtgtgg cgatcactct
    30721 gtggctgctt attttgtcgg tagtgatagg cggagtcctg gcgctgtttc tggcgattgg
    30781 tcgtgtctcc agtaataaat acatccagtt tccaatctgg ttatttacct atatttttcg
    30841 cggtacgccg ctgtatgttc agttgctggt gttctattcc ggcatgtaca cgcttgagat
    30901 tgttaaggga accgaattcc ttaacgcttt cttccgcagt ggcctgaact gtaccgtgct
    30961 ggcgctgacg cttaacacct gcgcttacac taccgagatt tttgctgggg caatccgttc
    31021 ggttccgcat ggggaaattg aagccgccag agcctatggc ttctcgactt ttaaaatgta
    31081 tcgttgcatt attttgcctt ctgcgctgcg tattgcgtta ccggcataca gcaacgaagt
    31141 gatcctgatg ctgcactcta ctgcgctggc gtttacggcc actgtgccgg atctgctgaa
    31201 aatagcccgc gatattaacg ccgccacgta tcaacctttt accgccttcg gcattgccgc
    31261 ggtgctctat ttaatcatct cttatgtcct gatcagcctc tttcgcagag cggaaaaacg
    31321 ctggttgcag catgtgaaac cttcttcaac gcactgagaa tacgatgtcc gagaataaat
    31381 taaacgttat cgatttgcac aaacgctacg gcgaacatga agtgctgaaa ggggtctcac
    31441 tgcaagcgaa tgccggagat gtaataagca tcatcggatc gtcgggatcg gggaaaagta
    31501 cctttctgcg ctgcattaac ttcctcgaaa aaccgagtga agggtcgatc gtggtcaatg
    31561 gccagacgat caatctggtg cgcgacaaag acggtcaact caaagtcgcc gataaaaatc
    31621 aactgcgctt actgcgcaca cgcctgacga tggtattcca gcacttcaat ctctggagcc
    31681 atatgacggt gctggaaaac gtcatggaag cgccgattca ggtgttgggc ctgagcaagc
    31741 aggaagcgcg cgagcgggcg gtgaagtatc tggcgaaagt cgggatagac gaacgtgcgc
    31801 aggggaaata tccggtgcat ctttccggcg gtcagcaaca gcgtgtttct atcgcgcggg
    31861 cgctggcgat ggaaccggaa gttttactgt ttgatgaacc tacctcggcg ctcgatcctg
    31921 aactggtagg cgaagtgtta cgtattatgc agcaactggc agaagagggg aaaaccatgg
    31981 tggtagtgac tcacgaaatg ggctttgctc gccatgtttc tactcacgtc attttcctcc
    32041 atcaggggaa aatagaagaa gagggcgcgc cggagcagtt atttggcaac ccgcaaagcc
    32101 ctcgtctgca acagttcctt aagggatcgc tgaaataatt tcctctccgg tggctaacgc
    32161 tgccggagta ttttcatggg gaatttgcga gtcagcttcc gctgaaatgt aaaagttgca
    32221 gtccgctaaa aatacttctg aattcgctgt ccaaagataa aaactgcctg ctgcattggg
    32281 tgttaatcgt ggttattgtc gcgtcatctt aaatttgagg ggtgacggaa cgccatgaca
    32341 atatcgacaa cttccacgcc gcatgatgcg gtatttaaat cttttttacg ccatccag
//
LOCUS       DAZPAC010000024        32115 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZPAC010000024 DAZPAC010000000
VERSION     DAZPAC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32115
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.024"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..692)
                     /gene="queC"
                     /locus_tag="ACHUPM_000926"
     CDS             complement(<1..692)
                     /gene="queC"
                     /locus_tag="ACHUPM_000926"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR0868786.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGL"
     gene            complement(757..2457)
                     /locus_tag="ACHUPM_000927"
     CDS             complement(757..2457)
                     /locus_tag="ACHUPM_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFR0868787.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQEQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            2557..3375
                     /gene="cof"
                     /locus_tag="ACHUPM_000928"
     CDS             2557..3375
                     /gene="cof"
                     /locus_tag="ACHUPM_000928"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR0868788.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            3527..3985
                     /gene="decR"
                     /locus_tag="ACHUPM_000929"
     CDS             3527..3985
                     /gene="decR"
                     /locus_tag="ACHUPM_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR0868789.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4015..5787
                     /locus_tag="ACHUPM_000930"
     CDS             4015..5787
                     /locus_tag="ACHUPM_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0868790.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            5780..7561
                     /locus_tag="ACHUPM_000931"
     CDS             5780..7561
                     /locus_tag="ACHUPM_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0868791.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            7742..8080
                     /gene="glnK"
                     /locus_tag="ACHUPM_000932"
     CDS             7742..8080
                     /gene="glnK"
                     /locus_tag="ACHUPM_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR0868792.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8110..9396
                     /gene="amtB"
                     /locus_tag="ACHUPM_000933"
     CDS             8110..9396
                     /gene="amtB"
                     /locus_tag="ACHUPM_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR0868793.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(9445..10305)
                     /gene="tesB"
                     /locus_tag="ACHUPM_000934"
     CDS             complement(9445..10305)
                     /gene="tesB"
                     /locus_tag="ACHUPM_000934"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR0868794.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            10523..11095
                     /locus_tag="ACHUPM_000935"
     CDS             10523..11095
                     /locus_tag="ACHUPM_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR0868795.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11126..11437)
                     /locus_tag="ACHUPM_000936"
     CDS             complement(11126..11437)
                     /locus_tag="ACHUPM_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR0868796.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            11600..11696
                     /gene="ffs"
                     /locus_tag="ACHUPM_000937"
     ncRNA           11600..11696
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHUPM_000937"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            11816..12169
                     /locus_tag="ACHUPM_000938"
     CDS             11816..12169
                     /locus_tag="ACHUPM_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR0868797.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(12211..13759)
                     /gene="pdeB"
                     /locus_tag="ACHUPM_000939"
                     /pseudo
     CDS             complement(12211..13759)
                     /gene="pdeB"
                     /locus_tag="ACHUPM_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(13923..14393)
                     /locus_tag="ACHUPM_000940"
     CDS             complement(13923..14393)
                     /locus_tag="ACHUPM_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR0868798.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(14509..15060)
                     /gene="maa"
                     /locus_tag="ACHUPM_000941"
     CDS             complement(14509..15060)
                     /gene="maa"
                     /locus_tag="ACHUPM_000941"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFR0868799.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(15231..15449)
                     /locus_tag="ACHUPM_000942"
     CDS             complement(15231..15449)
                     /locus_tag="ACHUPM_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR0868800.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(15475..15849)
                     /gene="tomB"
                     /locus_tag="ACHUPM_000943"
     CDS             complement(15475..15849)
                     /gene="tomB"
                     /locus_tag="ACHUPM_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR0868801.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(16395..19544)
                     /gene="acrB"
                     /locus_tag="ACHUPM_000944"
     CDS             complement(16395..19544)
                     /gene="acrB"
                     /locus_tag="ACHUPM_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR0868802.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(19567..20760)
                     /gene="acrA"
                     /locus_tag="ACHUPM_000945"
     CDS             complement(19567..20760)
                     /gene="acrA"
                     /locus_tag="ACHUPM_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR0868803.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            20902..21549
                     /gene="acrR"
                     /locus_tag="ACHUPM_000946"
     CDS             20902..21549
                     /gene="acrR"
                     /locus_tag="ACHUPM_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR0868804.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            21677..25039
                     /gene="mscK"
                     /locus_tag="ACHUPM_000947"
     CDS             21677..25039
                     /gene="mscK"
                     /locus_tag="ACHUPM_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR0868805.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            25100..25198
                     /locus_tag="ACHUPM_000948"
                     /pseudo
     CDS             25100..25198
                     /locus_tag="ACHUPM_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25251..25412)
                     /gene="rsmS"
                     /locus_tag="ACHUPM_000949"
     CDS             complement(25251..25412)
                     /gene="rsmS"
                     /locus_tag="ACHUPM_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR0868806.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(25426..25953)
                     /gene="priC"
                     /locus_tag="ACHUPM_000950"
     CDS             complement(25426..25953)
                     /gene="priC"
                     /locus_tag="ACHUPM_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR0868807.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26023..26400
                     /locus_tag="ACHUPM_000951"
     CDS             26023..26400
                     /locus_tag="ACHUPM_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR0868808.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            26553..27104
                     /gene="apt"
                     /locus_tag="ACHUPM_000952"
     CDS             26553..27104
                     /gene="apt"
                     /locus_tag="ACHUPM_000952"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR0868809.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            27233..29158
                     /gene="dnaX"
                     /locus_tag="ACHUPM_000953"
     CDS             27233..29158
                     /gene="dnaX"
                     /locus_tag="ACHUPM_000953"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR0868810.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    28491..28555
                     /gene="dnaX"
                     /locus_tag="ACHUPM_000953"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            29211..29540
                     /locus_tag="ACHUPM_000954"
     CDS             29211..29540
                     /locus_tag="ACHUPM_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR0868811.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            29540..30145
                     /gene="recR"
                     /locus_tag="ACHUPM_000955"
     CDS             29540..30145
                     /gene="recR"
                     /locus_tag="ACHUPM_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR0868812.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            30255..>32115
                     /gene="htpG"
                     /locus_tag="ACHUPM_000956"
     CDS             30255..>32115
                     /gene="htpG"
                     /locus_tag="ACHUPM_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR0868813.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQ"
BASE COUNT         7650 a         8142 c         8554 g         7769 t
ORIGIN      
        1 ctcaacccgg ttttctgctt catcgctgcc atcaccgtcg gtttatcggc cagataatga
       61 ttcaaaccgt tggcgcgtaa attacatgcc gcacaatgac cgcaaccgtc gcctttaatg
      121 ccgttatagc aggtcaacgt ttcgttacgg actaaatcca gtttgccgta ataatctgcc
      181 agcgcccagg tttccgcttt atcaatccac atcagcggcg tttcaaaacg aatatctttc
      241 gccatgccca aactgacggc atggtttagt gctttcacaa actcatcgcg gcaatccggg
      301 tagccggaga aatccgtttc acagacgccg gtaattacgg cttctgcttt tacctgatac
      361 gcatatattg ccgccagcgt caggaacaaa atattacgcc cagggacaaa cgtattcggg
      421 atgccatcgg cttcaggttc ataatcaggc accggaatgc tgtcacgtgt caggctgctg
      481 accgccagct cgttgagcag cgttacatca agaaccttat gcgcgcgtgc tcccagtttc
      541 agcgccagtt cgcgtgccac gtcgatttct gcgcgatgtc gctgaccgta atcgaacgtc
      601 acgcaatgga cttcatcata ttgttgtaat gcctgcacca gacaggtggt ggaatcctgg
      661 cctccactga acacaacgac agcacgtttc atagataatc ctgcttgaac aataaaagcg
      721 ttatggtaac gcctgcgatt aacccggacc agctattcat tgcgacggcg cgggaagcca
      781 ggcttcggta aattcaaacc agccgcgcgg tgtcagtcgc acaccgttca cgccgggagg
      841 ggcactaatg cgatagtgat agttgaacag cggcgtcagc gtcgcgtctg ccattaactg
      901 gctaaaaacc gctttcaggg cattaaatcg gttttcctca tcaggcatta cttgcaccgc
      961 gtccagcgtc gattgcagat gtgcatatgc tggagcgtcg aaaacatgtg gccatagtgg
     1021 atcgcaacgt agccattgct ccagagtata ttccggtgct tcgccaatta atctgtcgcc
     1081 catcatgagg tctgcgtgtg ccagtagggt cgtgtcgtcc cagttttttg cgttatgaaa
     1141 aataattgtg agttcacagc cttccgctgc cagtgtcgcc tgtaggcgtt ctgccatggt
     1201 atgaagttct atcggtaggt gataaaccag cgtcaaggtt ttcggtagtt tgacttcatc
     1261 cggtacttgc cagtgcggaa tagtccagcc tggcagtaat gcatgactgg cggtgatcag
     1321 gttttctccg acttctaacg tttgtaataa accggattga tgaataatgg agatcacttt
     1381 tcgcgcctgc cagagggaga gtcggggact tttgcgcaac gttaaatagc aaaaacctaa
     1441 actgatgcca ctactgacct ggctgacccg ttgcagctcc tccggtttgc cgatggtgat
     1501 ttgcacggga tgccgacaac tggttcccaa atctttttcg aaaagcggcg gagttatcca
     1561 gtactcaacc gctttaagca gcggatgacg caaatggtaa taatcatggc tctccaggcg
     1621 caccagctcc gctgtgaatt gtgttaatcg aaaaggacca gtgccgatca gtgggaattg
     1681 cggatgcgcc agatggctgc aatagctcgc cagccggtgc gcaagccagt aatcggggcg
     1741 atgtaaaaag aaggtcagac actgcggatg ggtgacttca atacgcttca cgctaataaa
     1801 taattgatcc agtgctggca gttgtaacag catcaataat cgctggtgta agtgtgaggc
     1861 ttttactgca tcgccgttat gccagtgtag ggttgaacga agataaaagt cccagcgtaa
     1921 cccgtcagta gaggtttccc tgtgatgcgc taaatcgcca atcgggcgct gagtattatt
     1981 atcgaagcgg gtcaggccgg aaaatatctg cccggcgaga tgctgctcgg cacggccggg
     2041 caaaaagcct ggttgtagcg gttcgagcgg gcgatagtag ggaatacgca acgtgggtgt
     2101 atcgttttgc cattgtccgc ccataaacgg ctgtaacaga gtgcgcagct cacctggggc
     2161 cagttgcgcc agctccagca catcttgctg ctttccggtt tccagtgcct gttccatcat
     2221 cgcattgcgt agcgattccg gcgtgaccag aaagcgtaat tgtccgcgct ttccgcgtcc
     2281 tgactgctcc tgccactcca gccatcccgc ctcctgtgcc tgacgcaaca gcgtacgaac
     2341 atggcgttcg ctgcaaaaac agcgttcggc cagttcgctg acggtgacgg tttgcggctt
     2401 tccggctgaa ggttgccaca gacgttgata ctggttaaga cggttgagca atcgcatata
     2461 aacccggaac aatattattt aactattcac tattacttcc gtatatatca ggtgatactc
     2521 aatctccatt aaccgtgtca cagagtggag aaagaaatgg ctcgtctggc agcatttgat
     2581 atggatggca ctttattgat gcctgaccat catttaggtg agaaaaccct ctctactttg
     2641 gcgcgactgc gtgaacgcga cattaccctc acttttgcca cggggcgtca tgcgctggag
     2701 atgcagcata ttctcggggc gctatcgctg gatgcatatt tgattaccgg caacggaacg
     2761 cgcgtgcatt ctctggaagg tgaactttta catcgtgatg atttacctgc ggatgtcgcg
     2821 gagctggtgc tgtatcagca atgggatacc cgagccagca tgcatatctt caatgacgac
     2881 ggttggttta ccgggaaaga gatccctgcg ttgttgcagg catttgtcta tagcggtttt
     2941 cgttatcaga taatcgatgt caaaaaaatg ccactcggca gcgtcaccaa gatctgcttc
     3001 tgtggcgatc acgacgatct tacacgcttg cagatccagc tatacgaagc attaggcgag
     3061 cgtgcacatt tgtgtttttc cgccacagat tgcctcgaag tgctgccggt gggctgcaat
     3121 aaaggcgctg cattgacggt gctgacccaa catttaggtt tatcgttgcg cgattgcatg
     3181 gcctttggtg atgcgatgaa cgatcgcgaa atgttaggca gcgtcggtag cgggtttatt
     3241 atgggcaatg cgatgccgca actgcgcgcg gagctcccgc atttaccggt gattggacat
     3301 tgccgaaatc aggctgtctc tcactatttg acgcactggc tggactatcc acatctacct
     3361 tattcccccg aataacgaga tcccttccag caccgggcaa ttgcccggtt tttttgcgtt
     3421 gaatttgtca ttttgtgccg tggtgtttaa accgcacaga ataaattgtc gtgatttcac
     3481 ctttaaaata gaattaaaag agaaaaaaat tctccgtgga agggctatgt tagataaaat
     3541 tgaccgtaag ctgctggcct tactgcagca ggattgcacc ctctctttgc aggcactggc
     3601 tgaagccgtt aatctgacaa ccaccccttg ctggaagcgc ctgaaacggc tggaggacga
     3661 cggtatcctt atcggcaaag tcgccctgct ggatccggaa aaaataggcc tcggcctgac
     3721 cgcctttgtg ctgataaaaa cgcaacatca cagcagcgaa tggtattgcc gctttgtcac
     3781 ggtggttacc gaaatgccag aagtgctggg gttctggcgc atggctggtg aatacgatta
     3841 tctgatgcgc gtccaggttg ccgacatgaa acgctacgac gagttttata agcgtctggt
     3901 aaacagcgtg ccggggctgt cggacgtcac ttccagcttc gcgatggaac agattaaata
     3961 caccacttct ttacccatcg aataaatatc cagaatcagg tcaggacaca acgcgtgcga
     4021 ttatttgctc aattaagctg gtatttccgt cgggaatggc gtcgctatct cggggctgtc
     4081 gccttgcttg tcattatcgc gatgctgcaa ctggttccgc caaaagtggt tggtattgtt
     4141 gtcgatggcg tgacagaaca acactttact accgggctga tcctgatgtg gatcgccacc
     4201 atggtgctga ttgccgttgt ggtttatctc ctgcgttatg tctggcgcgt attgctgttt
     4261 ggcgcgtctt accaactggc tgttgaactg cgtgaagatt attaccgtca gctaagccgg
     4321 cagcacccgg agttttacct gcgtcatcgc acgggtgatc tcatggctcg tgcgaccaat
     4381 gacgtcgatt gcgtcgtgtt tgccgccgga gaaggggtgc tgacgctggt ggattcactg
     4441 gtaatgggct gcgctgtgtt gattatgatg tctacgcaaa ttagctggca gttgacctta
     4501 tttgccctgt tgccgatgcc agtgatggcg atcatgatta agcgcaacgg cgatgctttg
     4561 catgaacgct ttaagctggc acaggcggcg ttttccagtc ttaatgaccg cacccaggaa
     4621 agcctcacca gtatccgcat gatcaaagcc tttggtctgg aagatcgcca gtcggcgtta
     4681 tttgccgcgg atgccgaaga taccggcaaa aaaaacatgc gggtggcgcg tattgatgct
     4741 cgtttcgacc cgaccatcta tatcgcgatt ggtatggcga acttgctggc gattggcggc
     4801 ggtagttgga tggtggtgca gggcagttta acgctgggcc agctcaccag ttttatgatg
     4861 tatttaggtc tgatgatttg gccaatgctg gcgctggcat ggatgtttaa cattgtggaa
     4921 cgtggtagtg ctgcgtacag ccgtattcgt gcgatgctgg cggaagcgcc ggtggtgaac
     4981 gatggtagcg aacccgtgcc ggaagggcgt ggcgaacttg atgtaaatat tcaccagttc
     5041 acgtatccgc agactgacca tcctgcgctg gaaaacgtca atttcgccct gaaacccggt
     5101 cagatgctgg gtatctgcgg gccgactggt tccggcaaaa gtaccctgtt gtcgctcatt
     5161 cagcgtcatt tcgacgttag cgagggggat attcgctttc atgatattcc tctgacgaag
     5221 ttacaactcg atagctggcg tagccgcctg gcggtggtca gccagacgcc attccttttt
     5281 tctgacaccg tggcaaataa catcgcgctg ggttgcccga atgccaccca gcaagagatt
     5341 gagcatgtcg cgcggttagc cagcgtacat gacgatattt tgcgtttacc gcaaggttac
     5401 gatacagagg tgggcgagcg cggcgtgatg ctttccggcg ggcaaaaaca gcgtatctct
     5461 attgctcgtg cgttattagt caacgcggaa atcctcatcc ttgatgatgc gctttcggcg
     5521 gtggacggac gcacagagca ccagatcctg cataacctgc gtcagtgggg acagggaaga
     5581 acggtaatca tcagtgccca tcgcctttcg gcactgacgg aagccagtga aattattgtg
     5641 atgcagcacg gacatatcgc ccagcgtggc aatcatgatg tgctggcgca acaaagcggc
     5701 tggtatcgcg atatgtatcg ctatcaacaa ctggaggcgg cgctcgacga cgctccggaa
     5761 aatcacgagg aggccgtcga tgcgtagttt tagccaactg tggccgactc tcaagcgcct
     5821 gttagcgtac ggttcaccgt ggcgtaaacc gctggggatt gcggtcctga tgatgtgggt
     5881 tgcggcggcg gcagaagtca gtgggccgct gcttatcagc tattttatcg acaatatggt
     5941 agcgaaaaat aacctgccgt tgaaagtggt tgcagggctg gctgcggcgt atgttgggct
     6001 acaactgttt gccgccgggc tacattacgc gcagtcgctg ctgtttaatc gggcggcagt
     6061 aggcgtagtg caacagttgc gtaccgacgt gatggatgct gcgttacgcc agccattaag
     6121 cgagtttgat acccaacccg tcgggcaggt gatttcccgc gtcactaatg acactgaagt
     6181 gatccgcgat ctctacgtta ccgtagtggc aactgtcctg cgcagtgccg cactggtggg
     6241 cgcgatgctg gtggcgatgt tcagcctcga ctggcgaatg gcgctggtgg cgataatgat
     6301 tttcccggta gtgctggtgg taatggtgat atatcaacgt tacagcacgc cgattgtccg
     6361 tcgtgtgcgc gcctatctgg cggatatcaa cgacggcttt aacgaaatca tcaatggcat
     6421 gagcgtcatc cagcagttcc gtcagcaggc gcgatttggc gaacgtatgg gtgaggccag
     6481 tcgttcacac tatatggcga ggatgcaaac cctgcgcctc gacggttttc tgctgcgtcc
     6541 gctgctgagt ctgttttcat cgctcattct ttgtggattg ttgatgctgt ttggcttctc
     6601 cgccagcggc accattgaag tgggcgtgct gtatgccttt atcagctatc ttgggcgact
     6661 taacgaaccc ttaatcgaac tgaccacgca acaggcgatg ctgcaacagg ctgttgttgc
     6721 tggtgagcgc gtgtttgaac tgatggacgg accgcgccag caatatggca atgatgatta
     6781 cccgttacag agtggcacca tcgaagtcga taacgtgtca tttgcttatc gcgatgacaa
     6841 tctggtgcta aagaacatta atctctctgt gccttcgagt aactttgtgg cgctggtcgg
     6901 gcataccggc agtggtaaaa gtaccctcgc cagtttattg atgggctatt acccgctaac
     6961 ggaaggtgag attcgccttg atggtcgtcc attaagttcg ctaagtcaca gcgcgctgcg
     7021 ccagggcgtg gcaatggtgc agcaagatcc ggtggtgctg gcggatacct tcctcgccaa
     7081 cgtgacgctg gggcgggata tctccgaaga acgcgtctgg caggcgctgg aaaccgtgca
     7141 actggcggag ctggcgcgta gcatgagcga cggtatttac acgccgctgg gcgagcaggg
     7201 gaataatctc tcagttgggc aaaagcaact gctggcactg gcgcgcgtgc tggtcgagac
     7261 gccgcaaatc ctgatccttg atgaggcaac cgccagcatt gactccggga ctgaacaggc
     7321 gattcaacat gctctggcgg cggtgcgtga acataccacg ctggtggtga ttgctcaccg
     7381 cttatcgacc attgttgatg ccgacaccat tctggtgctt catcgtgggc aagccgtgga
     7441 gcagggcact caccagcaac tgctggctgc ccagggacgc tactggcaga tgtatcaact
     7501 gcaacttgcg ggcgaagagc tggcagccag cgtgcgtgaa gaggaatcat tgagcgcctg
     7561 aatagcgcaa tatttcatcg ttggtgcaaa aatgtaacgc actgtgcact gtcatagtgc
     7621 gttttcattt tcaaacttct taacttcctg ctctctttct cgtttttcat ttctggcaca
     7681 ccgcttgcaa taccttcttc gtgtagcaga accattaccg aattctgacc ggaggggatc
     7741 tatgaagctg gtgaccgtga taatcaaacc attcaagctg gaagacgttc gtgaagcgtt
     7801 atcttccatt ggtattcagg gcctgaccgt caccgaagtg aaaggtttcg ggcgtcagaa
     7861 agggcatgcc gagctgtacc ggggggcgga atacagcgtc aacttcctgc caaaagtaaa
     7921 aattgatgtg gcgattgctg atgaccaact cgatgaagtg atcgatatcg tcagtaaggc
     7981 ggcttacacc ggaaaaattg gcgacggcaa aatcttcgtc gctgaattgc aacgcgtcat
     8041 tcgtattcgt accggcgaag ccgacgaagc ggcgctgtaa tctctggcac acagcaacag
     8101 gaacgaaaaa tgaagatagc gacgataaaa actgggcttg cttcactggc gatgctgccg
     8161 ggactggtaa tggctgcacc tgcggtggcc gataaagccg acaatgcgtt tatgatgatt
     8221 tgtactgcgc tggtgctgtt tatgactatt ccggggattg ccctgtttta cggtgggttg
     8281 attcgcggca aaaacgtgct gtcgatgctg acgcaggtga cggtgacgtt tgcactggtc
     8341 tgtattctct gggtggttta cggttactcg ctggcgtttg gtgagggcaa caacttcttc
     8401 ggcaacatta actggttgat gctaaaaaac atcgaactga cggcggtgat gggcagcatt
     8461 tatcagtata tccacgtggc gtttcagggc tcgtttgcct gcattaccgt cggcttgatc
     8521 gttggggcgc tggcggaacg aatccgcttc tcagctgtgt tgattttcgt ggtggtatgg
     8581 ctgacgctct cttacattcc gattgcgcat atggtgtggg gcggtggttt gctggcttct
     8641 cacggtgcgc tggatttcgc gggtggcacg gtggtgcata ttaacgccgc aatcgccggt
     8701 ctggtgggcg cgtatctgat aggcaaacgc gtgggcttcg gcaaagaggc gtttaaaccg
     8761 cacaacctgc caatggtctt caccggtact gccattctct atattggttg gtttggcttt
     8821 aacgccgggt cagcgggcac ggcgaatgaa atcgcggcac tggcatttgt gaatactgtg
     8881 gtcgcaacgg cggcggcaat tcttggctgg atcttcggtg aatgggcgct gcgtggtaag
     8941 ccttctctgc tgggagcgtg ttctggcgcg attgccggtc tggtcggcgt gacgccagcg
     9001 tgtggctaca ttggggttgg cggcgcgttg attatcggcg tggtagctgg tctggcgggc
     9061 ttgtggggcg ttactatgct caaacgcttg ctgcgggtgg atgatccctg cgatgtcttc
     9121 ggtgtgcacg gcgtttgtgg cattgtcggc tgtatcatga ctgggatttt tgccgccagt
     9181 tcgctgggtg gcgtgggctt cgctgaaggt gtgacgatgg gccatcagtt gctggtacag
     9241 ctggaaagca tcgccattac gatcgtctgg tccggtgtgg tggcatttat tggctacaaa
     9301 ctggcggatc tgacggttgg tctgcgtgtg ccggaagaac aggagcgaga agggctggac
     9361 gtcaacagcc acggcgagaa tgcctataac gcgtaacaag cactgcaaaa aacagccgga
     9421 cggttttcac ctccggctat ttttttaatt gtgattacgc atcacccctt cctgaacggt
     9481 cgaggcaacc agcacgccgt cctgagtata aaactcaccg cgcacaaagc cgcgtgcgct
     9541 ggacgccgag gtgctctcca cgctatacag cagccattca ttcaaattaa acgggcgatg
     9601 aaaccacatg gaatggtcaa tggtggcaat ctgaatcccc ggttcgagaa aaccgatgcc
     9661 gtgcggctgt agagctaccg gcaggaagtt aagatcagaa gcgtaaccga gcagatactg
     9721 atgaacgcgc aggtcatccg gcacgctacc atttgcgcgg atccacacct gacgatgtgg
     9781 ttctgcgacg tgacctttca gtgggttatg aaactccacc ggacggactt ccagcggacg
     9841 atcgcagatg aatttatctt tcagcactgg cggcagcagg tgcgccagcg attgggcgat
     9901 ttgtgtttcc gaaggcaggc catcaggcgc tggcgcggac ggcattgttt tttgatgttc
     9961 gaaacccgct tcaggtgcct ggaaagaggc cgtcatataa aaaatcggtt tgccgttttg
    10021 aatagcagca acccggcggg cgctgaagct gttaccgtca cgcagcgttt cgacatcata
    10081 aataatcggc ttcttactat cgccagggcg aagaaagtag ctgtgaaacg aatgtaccag
    10141 ccgctcttca gggacggtct cttttgcagc atacaaggcc tgacccacga cctggccgcc
    10201 aaacacctgg cgtaaaccta aatcttcact ctggccgcga aagagtcctt cctcaatttt
    10261 ttccagattt aacaatgtca gtaaattttt tagcgcctga ctcatataac tctccagtaa
    10321 ctaagctgcc gcagcaagcc aaagtgagtt gagtataacg caaatttgcc actggtccga
    10381 tgggtgcaat agtctgaatt acgggctaat tacaggcaga aatgcgtgat gtgtgccaca
    10441 cttgttgacg ttactatttt gttaaccact cttccggcga ggaaagttag cccgctggtg
    10501 cattgataat aaggagaagt gaatgaaact cgtgcacatg gccagttgtt tagcggttgc
    10561 gattgcgttg gcggcttgcg cagataaaag cgcggatatt cagacgccag caccggcggc
    10621 aaatacgtct atttcagcaa cacaacaacc tgctatccag caaccgaatg tctccggtac
    10681 cgtttggatc cgtcagaaag tcgcactgcc gcctgatgct gtgctgaccg tgacactttc
    10741 tgacgcgtcg ttagccgatg cgccgtcaaa agtgctggcg cagaaagcgg tgcgtaccga
    10801 aggtaaacag tcaccattca gctttgttct gccttttaac ccggcagatg ttcagccgaa
    10861 cgcgcgtatt ctgttgagtg cggcgattac cgtgaatgac aaactggtat ttattaccga
    10921 taccgttcag ccggtgatca acaagggcgg aactaaagcc gacctgacat tggtgccgat
    10981 gcagcaaacc gccgtgccgg ttcaggccag cggtggtgca acgactaccg taccttcgac
    11041 ttcaccaact caggtgaatc cgtcttcggc agttccagct cctacgcaat attaagcgac
    11101 gttaaccctc tccgcccgga gagggttagt agttccagcg ataacgctgc aagtcgattt
    11161 gcccgcttcc cgataccatc acaccttctg ccagtaatgc ctgtcgctga cgctgtaaat
    11221 ccggtccggt tagcgaaatt gtgccgtggc gattaaccac ccggtgccag ggtaaggtgc
    11281 tgccttcagg gagacgcttt aacacaccgc ccacctggcg cgcggcgcgg ggcgatcccg
    11341 ccagtttcgc cacatcaccg taagtggtga catagccttc gggaatagcg gcgacgattt
    11401 gccagacgcg ttggggaaat gaatcttctt tttccatctt ttcttcctga ggtaattttt
    11461 cagcataatc tggaaaaacg cccgagtgaa gtcgcattgc gcaagaaacc agcatctggc
    11521 acgcgatggg ttgcaattag ccggggcagc agtgataatg cgcctgcgcg ttggttctca
    11581 acgctctcaa tgggggctct gttggttctc ccgcaacgct actctgttta ccaggtcagg
    11641 tccggaagga agcagccaag gcagatgacg cgtgtgccgg gatgtagctg gcagggcccc
    11701 cacccatttc tgcctcccac cgtttcgtca aaaaatccca acatagctaa actttaacca
    11761 caactgacgt cgcaagaatt gtctggctgc gcagtacgct tcggaggtat gtctgatgaa
    11821 gtatgttgat ggttttgtgg ttgccgttcc tgccgataaa aaggatgcct atcgggaaat
    11881 ggccgctaag gctgcgccat tgtttaaaga gtttggcgcg cttcgtattg tcgaatgctg
    11941 ggccagcgat gtaccggatg gcaaagtgac cgattttcgt atggcggtga aagcggaaga
    12001 gaatgaagag gttgtcttta gctggattga atacccttca aaagaggtcc gcgacgctgc
    12061 taatcaaaag atgatgtcgg acccacggat gaaagagttc ggcgagtcca tgccgtttga
    12121 cggcaagcga atgatctatg gcggattcga gtcaatcatc gacgaatagg tagcgtgacg
    12181 ggctgtgcgg cgtgccgtca gcccaaaaat tcacaaatgt tgctcggccc agcgtaagaa
    12241 atcttctttc ggtaatgcct tgctgtagag ccagccctga ccgtagtgca cgccatgctg
    12301 gcgtagccac tcttcttgtt tactggtttc gattccctcc gctaccattt tcagtttcag
    12361 tgtttttgcc atttcgatga tatgcggcgt gacattttta tattccagcg catcaacgaa
    12421 agatttatcg atcttcagaa tgtcgacatc cagatcctgt aaataactta aacttgaata
    12481 tcccgtacca aaatcatcaa gataaatttc atggcccgcc tcccggtagc gagaaattat
    12541 cggggcgctg gttttcggat cggcaaactc gcgttcagtg agttcaagcg cgatctgtct
    12601 gggattaacc tgattgattg atcatttcac gcagcaattg cgggattttt tccgaggtga
    12661 gcacggtgga ttcaagattg atcgaaatat gctgctgtgg atgctgacgc agccagtcgc
    12721 ccatatcttc aaaggcgctt cttataatca gtagcgtcaa tggctcagaa aggcccgttt
    12781 gctgtgccag cggaataaaa ctatctggtg acaaccaact accgtctgtc tgcggccagc
    12841 gcgccagtgc ctcagcaccg acaattttgc cattggctaa ggagacaatc ggctgatagt
    12901 gcacgcaaat atcacgattt tcgatagcat cctgcagccg atgatgcggt gactggatac
    12961 ggcgcaaaat acgcagcaca aacatcgctg ccagcaggcc aatcaccaac ccgagcggta
    13021 accaaataaa gacttgccga tgccagcctt tctgcaacat tttcgttgaa gcccaggtga
    13081 tgatcgaaat attcatctcc gggaagggca ggatatcgta aatgattcca ttattttcga
    13141 tatgctcacc gggtgttttt tgtagcctgg taataattcc ctgagcaatt tcatcgctgc
    13201 tggttatgac aacgttatgg gcattgccaa taatggcggc atcaatttgc catgagctat
    13261 agggaatgac atcaataaag gaagcggggt cgatcatgac gacataatgt gccgttccca
    13321 tggcgaccat gtaacggata atgcctaaat cgttatgcga tgttaaccag acacgataac
    13381 catctttcga aattttacct ggctcgggga aggtatcggg cggactttca tgctccagag
    13441 acgaacactg gggaacgttg ttatcgatat aaaccacttc ctgaatatag cgataactgt
    13501 aagatacccg acgcatttcc atgagatggg cttcgctaca ggcagcgcct tgccatcttt
    13561 ccagctcttg cagcgcatct ttcccttgtg tcgccacctt attggcccga atagcgacgc
    13621 gggaggaata ggtatccagc tcttcaataa acgatgtttc tacctgctga tgggccagcc
    13681 agatgcttaa gccgacaggc agcaatactg aaagaatcag tactcccgaa ataaggccga
    13741 ccagatgtcg tgttctcacc agcatatcct tattaacaca gtgtagggaa agtatatctg
    13801 attgtggaaa aaggctggca ggagataagg aggtagagct ggtatttcat gaagtgatgg
    13861 gtggaaacga tcattgtcat ttgaccaagc gcagagcttt acgcctggta tgtaacccta
    13921 acttaagatg ttgattcggc gcgagcgagg gtaaaaatat cgtaaaaaga cagttcacct
    13981 ttacggacga tcattttttg cagttgttcc ttatgaacat ccgcgacacg cggagagttc
    14041 aagatggctt caaccagcgc ggcaggtaca aagcgcgtgt tgtaccattc gccgttatag
    14101 caaacgcgga aatccagcac gtcgatatct tcatagcggt agcgtgggta gacatcgaaa
    14161 aagaagaaac cattaagcag gataaataat acaaccagta gccagacaga gcctgacagc
    14221 gtttcggact gcaacatcac cgccagggtg gcaaaaaaag caacgtacat accgataaac
    14281 agccccggat gtttacggat aaaactgata ctaaagcggg gtttgttgtc gcgtttttcg
    14341 cgggtattca gagactcaat cgtttcggtc agcaggcgtt gtatttcggt catcgtttgc
    14401 cttcgtggta tctgcaactt tccagggaac accctatttt aacgtgggaa tagtagaggg
    14461 gaagtaacag atttaccaca attttgcata acagttgcga aaaaccgatt acaatttttt
    14521 aattattctg gctggattac cgcccacgac aacgttgtcc gggacatctt ttgtgacaac
    14581 tgcacctgag gctaccacga cgttatcacc aatggtcaca ccagggttaa tgaccgcgcg
    14641 tccgccaatc cagacgttat taccgatggt gacgggtttc cccagttcag caccgctatt
    14701 acgtgctaca gggtcgatgg gatgtgttgc cgtgtagata tgaacgcctg gtgccaacat
    14761 acagttatca ccgatgcgaa tagggcagac atcaagcatc acgcaatcga agttggcgaa
    14821 aaaattatta ccgagaaaaa tgttatagcc atagtcacag cgaaacgttg gctcaatata
    14881 agcctctgtc acctgaccga atagatcagc gagaatttgc tggcgtaatg tgtgctcttc
    14941 cgccagggaa tgattgtatc ggtgaataag ctgacgagcg cgcaggcgat cgcgagataa
    15001 cgtctcatct gccgagcgat acaactcacc agcaatcatc ttttcttttt ctgtgctcat
    15061 tactgacctc cggttgctga aaatgcggca acacagtaag acgaaaagtc agtgaagaga
    15121 acgttcccat tacatctgtg tgataaagat cacatagggt atgtcttcat ggtgaaaagt
    15181 ataaaattct taataaacag ccggttatag ctccgaaagc gaattattta ttagcgaata
    15241 aatttccata ctgaggaagg gatcttgtcg tatagtttat tcatggtcaa ttcggcgagg
    15301 cggtgatctg cggctgagta aaataccgcc agttcattat ctgataattc gtatttattt
    15361 ttctcgataa cacgctccag cgtgtcaatt gtctggcaac gacgtaaacg cattaaataa
    15421 tcggttttcg ttaaaggttt ttcggacata cttctaccca tggttgtaat aattctaaca
    15481 agataaactc gcaggattct ctttcgtcgc attgacaaaa caacggaata gtcgattacc
    15541 tgacttccgc catttctgaa gatcctgcat attaatacca taactactga acaacataaa
    15601 ggtgtcatcc agatattcgt cgatctgctc aatgagctta ttgtcttcat tatacttaat
    15661 tttgtaatta agtgcgaagg tctcaatatg ctcaatcagt tcattcaact ggaggttgat
    15721 cgccgaggtt gggtcgttaa cccagccatg attgctttct tcaaggtttg caaggcagtc
    15781 atgatacagg gtttcacaga gaaacttaag ctgtgcgata tcatgtcttt tgggtgagta
    15841 ttcatccata acgcgtcccc ttcttagcgg ttgaactaac ggacaccttt cgggatggaa
    15901 aaaacttact gacctggact tgccctctcg tttgttagct taactataag ccactctttg
    15961 caggttttca tcgcattatt acgaaaaatt acaattagga aggaatcggg acaggactca
    16021 ctttttcata ctagcactaa cccgcagcag gtaaaagcag tctgctcgaa ctgttcataa
    16081 catttcagga ttatcgccag atgaaattgg tgacactaat accaggattg ctctgaatat
    16141 gacgtaataa ccgaggaatg aataaagaat taccgcaaat aattaagaat agcctctaaa
    16201 tgattatgga ttattaaatc tattagcgct acttaatata aattaactaa caatcagtat
    16261 tttttatgaa ttttatccgt ggttaatact ggttttcgta tgagatcctg agttggtggt
    16321 tcaattactc cttaatgttc gtaggttatg cataaaaaag gccgcttgcg cggccttagt
    16381 gattacacgt tgtatcaatg atgatcgaca gtatggctgt gctcgatatc ttcattcttg
    16441 cggctaaagc ggcggcgaac caccacaaag aataccggaa cgaagaagat tgccagtacc
    16501 gttgcggtca ccatcccgcc cattacaccg gtacctactg cgttctgcgc gccggaacca
    16561 gcaccagtac tgataaccag cggcataacg ccgaggataa acgccagcga ggtcatcagg
    16621 atcggacgta aacgcatccg cactgcatca agcgtcgctt caatcagacc tttaccttct
    16681 ttatccatca agtctttggc gaattcgacg ataagtatcg cgttcttcgc cgacaaccca
    16741 atggttgtga gcaggcctac ctggaagtaa acgtcattgg tcaggccacg gaaggtggca
    16801 gccagcaacg caccgataac ccccagcgga acgaccagca taacggagaa cggaatcgac
    16861 cagctctcgt acagcgccgc cagacacagg aatacgacaa tcaacgaaat cgcgtacagt
    16921 gaaggtgcct ggttgccgga gagacgttcc tgataggaca tccccgtcca gtcatagcca
    16981 acaccggtag gcagtttgct cgccagttgt tccatcagct ccattgcttc accggtactt
    17041 ttacccggtg ccgcctggcc taagatttcc atggatggca ggccgttgta acgttccaga
    17101 cgcggcgaac cgtactccca acgagaagag gagaacgccg agaatggcac catctgacca
    17161 tcagcagcac gaacatacca gtcgccgata tcatccggca gcatacggta tttcgcttct
    17221 gacatgacgt aaactttctt cacacgaccg cggtcgataa agtcgttcac atagctaccg
    17281 ccccatgcag cgcccagaga ggtgttaatg tcgttgatag aaacacccag cgcctgcgct
    17341 ttttcctggt cgatatcaat cttaaactgc ggggtatctt ccagaccgtt tggacgtacg
    17401 ctggtcagca tatcagggtg cttcgctgct tctgcaagca gctggttacg cgcctgagtc
    17461 agtttttcgt gaccaaggcc agcctggtca atcagctcaa agtcaaagcc ggttgcagta
    17521 cccagttcca cgattgcggg caggttaaag gcgaaaacca tcgcatcttt gatttgcgag
    17581 aaagcgcgtg ttgcacgcat ggtaatcgct tcaactttgt tttcttcgcc cggacgatcg
    17641 gcccagtcct tcaaggaaac gaacgcaata ccggtattct gaccacgtcc cgcaaagccg
    17701 aagccgttaa cggcgaacac cgactcaacg ttgttctttt ctttggtcag atagtaatgc
    17761 gttacctcat tgagcacttt ctgcgtacgt tcctgcgttg cacctgctgg cagctgaacc
    17821 atggtcataa atacgccctg gtcctcatct ggcaagaagg agcttggcag acgcacgaac
    17881 agataggcca tgccgaccac gatgatcaga tacagcacca ggtaacgccc cgtactgcgc
    17941 agaataccgc ctacgctgtc ggtgtagtgg tgcgtgctct tctcgaacat gcggttaaac
    18001 cagccgaaga agcctttttt accttccccg tgatcgcctt tggcaatcgg tttcagcatg
    18061 gtggcacaaa gagccggagt caggatcaac gctaccagta ccgacagcgc cattgctgaa
    18121 acaatggtaa tagagaactg acgatagata gcaccagtag aaccgccaaa gaaggccatc
    18181 ggtacgaata ccgccgacag taccatcgcg ataccgacca gagcgccctg aatctgcccc
    18241 atcgacttac gggtagcttc ttttggcggc aaaccttctt ccgccataac acgctccacg
    18301 ttttctacca caacgatagc gtcatccacc aacaagccga tggcgagcac catcccgaac
    18361 attgttagcg tgtttatcga gaagccaaag gcggcaagga cggcaaaggt cccgagcaat
    18421 accaccggta cggcaatggt cggaatcaac gtcgcgcgga agttctgcag gaacagatac
    18481 ataaccagga acacgaggat gatcgcttcg accagcgttt taaccacttc gtgaatagag
    18541 attttcacga acggcgtggt gtcgtacggg taaacaattt tcagacccga cgggaagaac
    18601 ggttccatct tcgccagttc agcacggatt gccgcagcgg tatccagcgc gtttgcaccg
    18661 gtcgccagct tgatccccag accggaagcc ggttggccgt taaactctgc gatgatgtcg
    18721 tagttctcac cacccagctc aatcttcgcc acatcacgca gcagtacgcg ggaaccatcc
    18781 tgattcactt tcagcaggat tttgccgaac tcttcagtag aggtcagacg cgtctgagca
    18841 ataatagagg cgttaagctg ttggcctttc accggcggcg taccaccgag ctgacccgcc
    18901 gcaacctggg cgttctgcgc tttgatggcg gtaatgacat caaccggcgt tagctggaat
    18961 ttgttcagct cattcgggtt catccagata cgcatcgcgt actgtgaacc gaacaactga
    19021 acatcaccca cgcccgacgt acggctgatg gcatctttca tattcgccgc tacgtagtca
    19081 gagatatcct cctgcgtcat ggtgccatcg gtgttgataa cgccgacaac catcaggaag
    19141 ctgctggatg atttctcaac gctcacccct tgctgctgaa cttcttgcgg cagcaacggc
    19201 atcgccagct gcagtttgtt ctgcacctga acctgcgcga tatccgcatc agtaccagac
    19261 tcaaaggtca gggtgatctg cacggtaccc gtggagtcac tgttagagga catgtacatc
    19321 aggttatcga taccgttcat attctgttcg ataacctgtg tcaccgtgtc ctgcactgtt
    19381 ttcgcatcag cgccggggta ggaggcggag atcgttactg ccggcggtgc aatcgtagga
    19441 tattgcgcca ccggcagttt gaggatcgcc agcccccctg ccaacatgat gataatggcg
    19501 atcacccacg caaaaatcgg gcgatcgata aagaaattag gcatgtctta acggctcctg
    19561 tttaagttaa gacttggact gttcaggctg agcaccgctt gcggcttgct ggttattatc
    19621 agcggtaact tcttgtgctt ttacctggac accaggacgc actttctgca gcccacttat
    19681 tactacgcga tcgcctgctt tcagaccttc tgtcaccagc cacttatcgc caatagcctg
    19741 gcttgcaacg atcggacggg tttccacttt gtcatccgcg ccaactacca gtacggtggc
    19801 atcaccacgc ggcgtacggg ttacgccctg ttgcgggact aaaatagcgt ttggattaag
    19861 cccttcttcc agacgtgcgc gcacgaacat acccggcagc agagtgtgat ccgggttcgg
    19921 gaagatagcg cgtagggtga tagacccagt ggtctgatca acggtaacgt cagagaattc
    19981 cagcgtaccg tcctgcggga acttaatgcc gtcactggtg atcagcgaca ctttggcttt
    20041 gccgttctct tgtttcagcg tgccattcgc cagttcctgt ttcaggcgca ggaagtcgtt
    20101 gctggactgg gtcacatcaa ggtagatcgg atcaagttgc tgcacggttg ccagcgcagt
    20161 cgcctgaccg ttctgtacca atgcgccttc cgtcacgttc gatttaccaa tgcgaccgct
    20221 aatcggagag gtgactttgg tgtaagccag attgatccgc gcagtttcaa cggcagcttt
    20281 cgccgcagtt accgcagcat tcgcctgttg cgcatcagcc agagcctgat cgtactcttg
    20341 cttactgatg tactgagtac cgagcagttt ctgataacga ttcaccgtca attgcgcgat
    20401 attggctgca gcctgggctt tcgccagatc acctttcgca ctgtcgtatg tcgcctgata
    20461 ggtcgcagga tcaatctgat agagagagac accagcttcg atgtcgctac cttctttgaa
    20521 attacgcttc aggataatcc cgctaacttg aggacgaact tctgcgatcc ggtaggcact
    20581 ggtgcgaccc ggaagctcgg ttgtgatctg cagaggttca gttttgactg ttactacgcc
    20641 aacggcgggc atctgctggc caccttgttg ggcctgtttg tcgtcacatc ctgttagggc
    20701 taagctgcct gagagcatca gaacgaccgc cagaggcgta aaccctctgt ttttgttcat
    20761 atgtaaacct cgagtgtccg atttcaaatt ggtcaatggt caaaagttaa taaacccatt
    20821 gctgcgttta tattatcgtc gtgctatggt acatacattc acaaatgtat gtaaatctaa
    20881 cgcctgtaaa ttcacgaaca tatggcacga aaaaccaaac aagaagcgca agaaacgcgc
    20941 caacacatcc tcgatgtggc tctacgtctt ttctcacagc agggggtatc atccacctcg
    21001 ctgggcgaga ttgcaaaagc agctggcgtt acgcgcggtg caatctactg gcattttaaa
    21061 gacaagtcgg atttgttcag tgagatctgg gaactgtcag aatccaatat tggtgaacta
    21121 gagcttgagt atcaggcaaa attccctggc gatccactct cagtattaag agagatatta
    21181 attcatgttc ttgaatccac ggtgacagaa gaacgacgtc gattattgat ggagattata
    21241 ttccacaaat gcgaatttgt cggagaaatg gctgttgtgc aacaggcaca acgtaatctc
    21301 tgtctggaaa gttatgaccg tatagaacaa acgttaaaac attgtattga agcgaaaatg
    21361 ttgcctgcgg atttaatgac gcgtcgggca gcaattatta tgcgcggcta tatttccggc
    21421 ctgatggaaa actggctctt tgccccgcaa tcttttgatc ttaaaaaaga agcccgcgat
    21481 tacgtcgcca tcttactgga gatgtatctc ctgtgcccca cgcttcgtaa tcctgccact
    21541 aacgaataac cctgaatctg actccaggat ttttcctgga cattttcgtc gttgctattc
    21601 tggttcactg cgtcgtgata ttcttgcggt ttgacttttt caggtcgttc ttcaggttca
    21661 gaaaccttca ttcatcatga ctatgttcca gtattacaaa cgatcacggc attttgtttt
    21721 ttcagcattt attgcttttg tttttgtctt gttatgccag aacacggcgt ttgcgcgggc
    21781 gtcatcgaat ggtgatctgc cgacaaaagc ggacctgcag gcgcaacttg actcactaaa
    21841 taaacaaaaa gatctttctg ctcaggacaa actggtgcag caggatctga cagatacatt
    21901 agccaccctc gataaaatcg atcgcataaa agaagagaca gttcagctac ggcaaaaagt
    21961 cgctgaagcg ccggaaaaaa tgcgccaggc gaccgcggcg ttaacagcac ttagcgatgt
    22021 cgataacgac gaagaaacgc gcaaaattct gagtacgctt tcgttgcgcc agctggaaac
    22081 tcgcgttgcc caggcgctgg acgatttgca aaacgcacaa aacgatctgg cgtcttataa
    22141 cagccagctg gtttcgttac agacgcagcc cgaacgcgtg caaaatgcga tgtataacgc
    22201 ttcgcagcag ctgcaacaaa ttcgcagtcg tctggatggg actgatgtcg gcgagactgc
    22261 cttacgtccc agccagaaag tgttaatgca ggctcagcag gcgttgctga atgcggagat
    22321 tgaccagcag cgtaaaagcc tggaagggaa caccgtcttg caggatacct tgcaaaagca
    22381 acgtgattac gtgacggcga acagcgcccg tctggagcac cagttgcagc tgttgcaaga
    22441 agcggtaaac agcaagcgcc tgactttaac tgaaaaaacg gcgcaggaag ccgtctcccc
    22501 ggatgaagcc gcgcgtattc aggctaatcc gctggtgaaa caggaactgg aaattaacca
    22561 gcagttaagt cagcgtctga ttaccgcgac tgaaaacggt aatcagttga tgcagcaaaa
    22621 cattaaagtc aaaaactggc tggagcgggc gctgcaatcg gaacgcaata ttaaagagca
    22681 gattgccgtc ctgaagggca gcctgctgtt gtctcgtatc ctttaccagc aacaacaaac
    22741 gctgccctcg gcggatgaac tggaaaacat gaccaaccgc atcgcggatt tgcgtctcga
    22801 acagtttgaa gttaaccagc agcgtgatgc actcttccag agcgatgcgt tcgtcaacaa
    22861 actggaagaa ggtcacacca acgaagtcaa cagcgaagtt cacgatgcgt tattgcaagt
    22921 ggttgatatg cgtcgcgaat tgctggatca actcaacaaa cagttgggta accagctgat
    22981 gatggccatt aacctgcaaa tcaaccagca gcagttaatg agcgtatcga aaaacctgaa
    23041 atccatcctg actcagcaaa tcttttgggt gaacagtaac cgtccaatgg actgggactg
    23101 gatcaaagcg ttcccgcaaa gcctgaaaga tgaatttaag tcgatgaaaa tcacggtgaa
    23161 ctgggaaaaa gcctggcctg ccgtttttat cgctttcctc gccggtttgc cgctgctgtt
    23221 gattgccggg ctgatccact ggcgtctggg ctggctgaaa gcgtatcaac agaaactggc
    23281 ttccgctgtg ggttccctgc gtaacgatag ccagctcaac acaccaaaag cgatccttat
    23341 cgacctgatc cgcgcgctgc cggtgtgcct gattattctc gcggttggcc tgattctgtt
    23401 gaccatgcag ctcaacatca gcgaactgct atggtcgttc agtaaaaaac tggcgatttt
    23461 ctggctggtg tttggcctgt gctggaaggt actggagaaa aatggcgttg ccgtgcgtca
    23521 cttcggcatg ccggagcagc agaccagcca ctggcgtcgg caaattgtcc gcatcagtct
    23581 cgcgttgctg cctatccatt tctggtctgt ggtggcagaa ctttccccgc tgcatctgat
    23641 ggatgatgtg ctggggcagg cgatgatttt cttcaacctg ctgctgattg ccttcctggt
    23701 atggccaatg tgccgcgaaa gctggcgtga taaagagtcg cacaccatgc gactggtcac
    23761 cattaccgtg ctgtcgatta tcccgattgc attgatggtg ctgactgcca cgggttattt
    23821 ctacaccacg ctgcgcctct ccgggcgttg gattgaaacc gtttatctgg tgatcatctg
    23881 gaacttgctc taccagacgg tattgcgcgg cttaagcgta gcggcgcggc gtatcgcctg
    23941 gcgtcgtgcg ctggcgcgtc ggcagaatct ggtgaaagag ggcgcagaag gtgccgaacc
    24001 gccggaagaa cccaccattg cactggaaca ggttaaccag cagacgctgc gtattaccat
    24061 gttgctgatg tttgcgctgt tcggtgtcat gttctgggca atttggtccg atttgatcac
    24121 cgtgttcagc tatctcgaca gcatcacgct ctggcattac aacggcactg aagctggcgc
    24181 tgcggtggtg aaaaacgtca ccatgggcag tctgttgttt gcgattatcg cctcaatggt
    24241 ggcctgggcg ttgattcgca acctgcctgg tttactggaa gtgctggtgc tctcgcgact
    24301 gaatatgcgt cagggcgcgt cgtatgccat tactaccatc cttaactaca tcattattgc
    24361 cgttggtgcg atgacggtgt tcggatcgct gggcgtctct tgggataaac tccagtggct
    24421 ggccgcagca ttatccgtag gtcttggttt tggtttacaa gaaattttcg gtaacttcgt
    24481 ctccggtttg atcattctgt tcgaacgtcc ggtgcgtatt ggcgatacgg taaccattgg
    24541 tagcttctcg gggacggtaa gtaagatccg tattcgtgcg acaacgatta ccgatttcga
    24601 tcgcaaagaa gtgatcatcc cgaacaaagc gtttgttacc gagcgtctga tcaactggtc
    24661 gttgactgac actactacgc gtctggtgat ccgtctcggc gtggcctatg gctccgatct
    24721 ggaaaaagtg cgtaaagtgt tactgaaggc ggcgactgag cacccaaggg tgatgcacga
    24781 accaatgccg gaagtcttct ttacggcgtt tggtgccagc acgttggatc atgagctgcg
    24841 tctgtatgtg cgtgaactgc gtgaccgtag tcgtactgtc gatgagctga accgtactat
    24901 cgatcagctg tgccgtgaaa acgacatcaa cattgccttt aaccagcttg aagtgcatct
    24961 gcacaacgag aagggcgatg aggtgacgga agtcaaacgc gactacaaag gcgatgaccc
    25021 gacgccagcg gtagggtaat aaccaaaggg ggcgacattt agttgccccg agattactga
    25081 caaagtgcgc gttgttcatg ccggatgcgg cgtgaccgcc ttatccggcc tacgaaaccg
    25141 caagaattca atgtattgca ggagcggtgt aggcctgata agcgtagcgc atcaggcagt
    25201 tttgcgtttg cccacaatct taggggacat ttagcggccc catttatttc tcacttttcc
    25261 gccttatcat cgcgcgttaa tttcttttca taatcgcgtt ttacaatatc cagcgcgcgc
    25321 agcacggtgc gggctgggat ctgattttct tccagcagca caatcaaatc gacagccagt
    25381 ttgacatcgt caggggcatt ttcaagtgac atactctctc cattgctagc gggttaagcg
    25441 cgctagcctg ttttcgattt tttccagcgc atggcggcag cgtgccaggc gcgcttcata
    25501 ggcttccact tcacgatgca gcgtttgctg ttccacgaga tcggtcaccc gcgccaggcg
    25561 ggctctgcgt tcggcaacca tctcacgtag ccgccgctca aaatcctgat gctgaatacg
    25621 tttacgctgc cagcgggcaa ttttcggtgg cgcactgtcc cactcgcgca atgaccaggc
    25681 ggaggcttca cgcgcgatgg cttccagttg tgccgccaga tgttccgcca gccaggccac
    25741 ttgcggcagc tgttgctgct caaccgcatg acaaagcgca gccagattat cgcccgcctc
    25801 gtcgagacaa gcctgtagtg ttgtcgcacg agtctgaaaa agatgcctgt cgaaacgagc
    25861 acttagcgtg gcgaactgtg acaccggggc acaacgctga cgcagcgtag cgagctgtcc
    25921 ttccagtttt tccagcagca gggcggtttt cacgataaga cctcaatgaa aatgataatt
    25981 gttatgctaa agtagccact ctgtaagctg acaacactca atatgcaacg aatcatttta
    26041 atcatcattg gctggctggc ggtagtgctg ggtacgctgg gcgtggtatt accggtatta
    26101 ccgacgacgc cgtttatcct gctggcggcc tggtgctttg cccgttcttc cccgcgcttt
    26161 cacgcctggt tgctgtaccg ctcatggttt ggcagctatc tacgtttctg gcagaaacat
    26221 catgcgatgc cgcgcggcgt caaaccgcgg gcgattttgc ttattttgct cacgtttgcc
    26281 atttctctgt ggttcgtcca gatgccatgg gtgcgcatca tgttgctggt aattctcgcc
    26341 tgtttgcttt tctatatgtg gcgaattccg gtgattgatg aaaagcaaga aaagcactga
    26401 agcacaacaa tcgcagttgc aattattgcg tacagccagt acattctggc gttttcgagc
    26461 acaggcgcag gcggtcaaag gttaaacaac tgttactttt gatacgttta aaacgcgccg
    26521 tgagtaccac cgtaacaagc aggcatacac ttatgaccgc gactgcacag cagcttgagt
    26581 atctcaaaaa tagcatcaaa agcattcagg actacccaaa acccggcatt cttttccgcg
    26641 atgtcaccag cttactggaa gacccgaaag cttacgctct cagcatcgac ttgctggttg
    26701 agcgttacaa aaatgcgggc attaccaaag ttgtcggcac cgaagcgcgt ggcttcttgt
    26761 ttggcgctcc ggtagctctg ggtctgggcg ttggctttgt accggtccgt aaaccgggca
    26821 aactgccgcg tgaaaccatc agtgaaactt acgacctgga atacggcacc gatcagctgg
    26881 agatccacgt tgatgccatc aaaccgggcg acaaagttct ggtggtggac gatctgctgg
    26941 caaccggcgg cactatcgaa gcgaccgtta aactgatccg tcgtctgggt ggtgaagtgg
    27001 ctgacgctgc gttcattatc aacctgttcg atctcggcgg cgaacagcgt ctcgaaaaac
    27061 agggcattac cagctacagc cttgtcccgt tcccgggcca ttaattatcg ccagtcttgt
    27121 gctgcccacg ctacggacag cacaagatgt gcattcagcc tcgccgttct gacggggctg
    27181 tgttagcatt accccttcgt gaatccacct tccagcgttt cagagcctgc caatgagtta
    27241 tcaggtctta gcccgaaaat ggcgcccaca aacctttgct gacgtcgtcg gccaggaaca
    27301 tgtgctgacc gcactggcga acggcttgtc gttagggcgt attcatcatg cttatctttt
    27361 ttccggcacc cgtggcgtcg gaaaaacctc tatcgcccga ctgctggcga aggggctaaa
    27421 ctgcgaaacc ggcattaccg cgacgccgtg cggcgtgtgc gataactgtc gtgaaatcga
    27481 gcaggggcgc tttgtcgatc tgattgaaat cgacgccgcc tcgcgcacca aagttgaaga
    27541 tacccgcgac ctgctggata acgtccagta cgctccggcg cgtggtcgtt tcaaagttta
    27601 tctgatcgac gaagtgcata tgctgtcgcg ccacagcttt aacgcactgt taaaaaccct
    27661 tgaagagccg ccggagcacg ttaagtttct gctggcgacg accgatccac agaaattgcc
    27721 ggtgacgatt ttgtcgcgct gtttgcaatt tcatctcaag gcgctggatg tcgagcaaat
    27781 tcgccatcaa cttgagcaca tcctcaacga agaacatatc gctcacgagc cgcgggcgtt
    27841 gcaattgctg gcgcgcgccg ctgaaggcag cctgcgagat gccttaagtc tgaccgacca
    27901 ggcgattgcc agcggtgacg gccaggtttc aacccaggca gtcagtgcga tgctgggtac
    27961 gcttgatgac gatcaggcgc tgtcgctggt tgaagcgatg gtcgaggcca acggcgagcg
    28021 cgtaatggcg ctgattaatg aagccgctgc ccgtggtatc gagtgggaag cgttgctggt
    28081 ggaaatgctc ggcctgttgc atcgtattgc gatggtacaa ctttcgcctg ctgcacttgg
    28141 caacgacatg gccgccattg agctgcggat gcgtgaactg gcgcgcacca taccgccgac
    28201 ggatattcag ctttactatc agacgctgtt gattggtcgc aaagaattac cgtatgcgcc
    28261 ggaccgccgc atgggcgttg agatgacgct gctgcgcgcg ctggcgttcc atccgcgtat
    28321 gccgctgcct gagccagaag tgccacgcca gtcctttgca cccgtcgtgc caacggcagt
    28381 aatgacgcca acccaggtgc cgccgcaatc agcgccgcag caggcaccga ctgtaccgct
    28441 cccggaaacc accagccagg tgctggcggc gcgccagcag ttgcagtgcg tgcagggagc
    28501 aaccaaagca aaaaagagtg aatcggcagc cgctacccgc gcgcggccgg tgaataacgc
    28561 tgcgctggaa agactggctt cggtcaccga tcgcgttcag gcgcgtccgg tgccatcggc
    28621 gctggaaaaa gcgtcagcca aaaaagaagc gtatcgctgg aaggcgacca ctccggtgat
    28681 gcagcaaaaa gaagtggtcg ccacgccgaa ggcgctgaaa aaagcgctgg aacatgaaaa
    28741 aacgccggaa ctggcggcga agctagcggc agaagccatt gagcgcgacc cgtgggcggc
    28801 acaggtgagc caactttcgc taccaaaact ggtcgaacag gtggcgttaa atgcctggaa
    28861 agaggagagc gacaacgcag tatgtctgca tttgcgctcc tctcagcggc atttgaacaa
    28921 ccgcggtgca cagcaaaaac tggctgaagc gttgagcatg ttaaaaggtt caacggttga
    28981 actgactatc gttgaagatg ataatcccgc ggtgcgtacg ccgctggagt ggcgtcaggc
    29041 gatatacgaa gaaaaacttg cgcaggcgcg cgagtccatt attgcggata ataatattca
    29101 gaccctgcgt cggtttttcg atgcggagct ggatgaagaa agtatccgcc ccatttgatc
    29161 gtaagcacag cttacgttcg tcatccttaa cgtgattgag agagaaacct atgtttggta
    29221 aaggcggtct gggtaacctg atgaagcaag cccagcagat gcaagaaaaa atgcagaaaa
    29281 tgcaggaaga gatcgcgcag ctggaagtca ccggcgaatc tggcgcaggt ctggtaaaag
    29341 tgaccatcaa cggtgcacac aactgccgtc gcgtagagat cgacccgagc ctgctggaag
    29401 acgacaaaga gatgctggaa gacctggtgg ctgcagcatt caacgacgca gcacgtcgta
    29461 ttgaagaaac gcagaaagaa aaaatggcct ctgtatcctc cggaatgcag ctgccgcctg
    29521 gctttaagat gccgttctga tgcaaaccag cccgctgtta acacagctta tggaagcact
    29581 gcgctgtctg ccgggcgttg gcccgaagtc ggcgcagcgt atggcgttca cgctgcttca
    29641 gcgcgatcgt agcggcggga tgcgtttggc gcaggcgctc acccgggcga tgtcggaaat
    29701 cggccactgc gccgattgcc gcactttcac cgaacaggaa gtctgtaaca tctgttcgaa
    29761 tccgcgtcgt caggaaaacg gtcaaatctg tgtggtggag agtccggcgg acatctacgc
    29821 cattgagcag acggggcagt tttctggtcg ttattttgtg ttgatggggc atctgtcacc
    29881 gctggacggc atcggtccgg atgatatcgg gcttgatcgt ctggaacagc gtctggcaga
    29941 ggaaaaaatc actgaagtga tcctcgccac caatcccacg gttgaaggtg aagctaccgc
    30001 taactacatt gccgagcttt gcgcgcaata tgacgtggaa gccagccgaa tcgctcatgg
    30061 cgtaccggtt ggcggcgaac tggaaatggt cgatggcacc acgctgtcac actcccttgc
    30121 cgggcgtcat aagattcgtt tttaagcaaa cgagagcagg atcacctgct ctcgcttgaa
    30181 attattctcc cttgtcccca tctctccctc atcctgtttt taaccttaaa atggcattat
    30241 tgaggtagac ctacatgaaa ggacaagaaa ctcgtggttt tcagtcagaa gtgaaacagc
    30301 ttctgcacct gatgatccat tctctctatt ccaataaaga aatcttcctg cgtgagctta
    30361 tctctaacgc ctccgatgcg gcggacaagc tgcgtttccg tgcgctatct aacccggacc
    30421 tgtacgaagg tgatggcgaa ctgcgcgttc gtgtctcttt cgataaagac aagcgcacgc
    30481 tgaccatctc tgataacggc gtggggatga cccgcgacga agtgattgac catctgggga
    30541 ctatcgctaa atccggtacc aaatcattcc tcgaatccct gggttctgac caggcgaaag
    30601 acagccagct gatcggtcag tttggtgttg gtttctactc tgcgtttatc gtggccgaca
    30661 aagtgaccgt gcgtactcgc gcggcaggcg aaaaaccaga aaacggcgtc ttctgggaat
    30721 cggctggcga aggtgaatat accgttgccg acatcaccaa agaagatcgt ggtactgaaa
    30781 tcaccctgca tctgcgtgaa ggcgaagacg agttcctcga tgactggcgc gtgcgttcca
    30841 tcatcagcaa atactccgac catatcgcgc tgccggtaga gatcgaaaaa cgcgaagaga
    30901 aagacggcga aaccgttatc tcctgggaga aaatcaacaa agcgcaggcg ctgtggactc
    30961 gtaacaagtc ggaaatcacc gatgaagagt acaaagagtt ctacaaacac atcgcccacg
    31021 actttaatga tccgctgacc tggagccaca accgcgttga aggtaagcag gagtacacca
    31081 gcctgctgta cattccgtcc caggctccgt gggatatgtg gaaccgtgat cataaacacg
    31141 gcctgaaact gtacgtccag cgtgtgttca tcatggacga cgcagaacag ttcatgccga
    31201 actatctgcg cttcgtgcgt ggtctgattg actccagcga tctgccgctg aacgtttccc
    31261 gtgaaatcct ccaggacagc acggtaacgc gtaacctgcg caatgcgctg accaagcgtg
    31321 tgctgcaaat gctggaaaaa ctggcgaaag acgacgcgga aaaataccag accttctggc
    31381 aacagtttgg cctggtactg aaagaaggtc cggcggaaga tttcgctaac caggaagcga
    31441 tcgccaaact gctgcgtttt gcttctaccc ataccgattc ttctgcgcag accgtatctc
    31501 tggaagacta cgtttcccgc atgaaagaag ggcaggagaa aatctactac atcaccgcag
    31561 acagctatgc ggcagcgaag agcagcccgc acctggaact gctgcgtaag aaaggcatcg
    31621 aagttctgct gctttccgac cgcatcgatg agtggatgat gaactatctg actgagttcg
    31681 acggtaaacc gttccagtcg gtgtctaaag ttgacgagtc gctggaaaaa ctggctgacg
    31741 aagttgatga gagcgcgaaa gaagcggaga aagcactgac tccgttcatc gaccgtgtga
    31801 aagccctgct cggcgagcgc gtgaaagatg tccgtctgac tcaccgtctg accgatacgc
    31861 cagcgatcgt ttcgaccgac gcggacgaaa tgagcactca gatggcgaaa ctgttcgctg
    31921 cggcgggcca gaaagtgcca gaagtgaaat acatcttcga actgaacccg gatcacgtac
    31981 tggtgaaacg tgcggcagat actgaagatg aagcgaagtt cagcgagtgg gtagaactgc
    32041 tgctggatca ggcgctgctg gcagaacgcg gcacgctgga agatccgaac ctgtttattc
    32101 gtcgtatgaa ccagc
//
LOCUS       DAZPAC010000025        31869 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZPAC010000025 DAZPAC010000000
VERSION     DAZPAC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31869
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.025"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(3..437)
                     /gene="slyA"
                     /locus_tag="ACHUPM_000957"
     CDS             complement(3..437)
                     /gene="slyA"
                     /locus_tag="ACHUPM_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR0868814.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            638..874
                     /locus_tag="ACHUPM_000958"
     CDS             638..874
                     /locus_tag="ACHUPM_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR0868815.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            877..1734
                     /locus_tag="ACHUPM_000959"
     CDS             877..1734
                     /locus_tag="ACHUPM_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR0868816.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1755..3746
                     /locus_tag="ACHUPM_000960"
     CDS             1755..3746
                     /locus_tag="ACHUPM_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR0868817.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3747..4268)
                     /gene="sodC"
                     /locus_tag="ACHUPM_000961"
     CDS             complement(3747..4268)
                     /gene="sodC"
                     /locus_tag="ACHUPM_000961"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR0868818.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(4349..5245)
                     /locus_tag="ACHUPM_000962"
     CDS             complement(4349..5245)
                     /locus_tag="ACHUPM_000962"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR0868819.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(5294..5533)
                     /locus_tag="ACHUPM_000963"
     CDS             complement(5294..5533)
                     /locus_tag="ACHUPM_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR0868820.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            5636..6235
                     /gene="nemR"
                     /locus_tag="ACHUPM_000964"
     CDS             5636..6235
                     /gene="nemR"
                     /locus_tag="ACHUPM_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR0868821.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            6272..7369
                     /gene="nemA"
                     /locus_tag="ACHUPM_000965"
     CDS             6272..7369
                     /gene="nemA"
                     /locus_tag="ACHUPM_000965"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR0868822.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            7450..7857
                     /gene="gloA"
                     /locus_tag="ACHUPM_000966"
     CDS             7450..7857
                     /gene="gloA"
                     /locus_tag="ACHUPM_000966"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR0868823.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7960..8607
                     /gene="rnt"
                     /locus_tag="ACHUPM_000967"
     CDS             7960..8607
                     /gene="rnt"
                     /locus_tag="ACHUPM_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR0868824.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            8700..13269
                     /locus_tag="ACHUPM_000968"
                     /pseudo
     CDS             8700..13269
                     /locus_tag="ACHUPM_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(13320..13667)
                     /gene="grxD"
                     /locus_tag="ACHUPM_000969"
     CDS             complement(13320..13667)
                     /gene="grxD"
                     /locus_tag="ACHUPM_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR0868825.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            14001..14816
                     /gene="mepH"
                     /locus_tag="ACHUPM_000970"
     CDS             14001..14816
                     /gene="mepH"
                     /locus_tag="ACHUPM_000970"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR0868826.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            14944..15525
                     /gene="sodB"
                     /locus_tag="ACHUPM_000971"
     CDS             14944..15525
                     /gene="sodB"
                     /locus_tag="ACHUPM_000971"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR0868827.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(15671..16840)
                     /locus_tag="ACHUPM_000972"
     CDS             complement(15671..16840)
                     /locus_tag="ACHUPM_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0868828.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(17006..17095)
                     /gene="cydH"
                     /locus_tag="ACHUPM_000973"
                     /gene_synonym="ynhF"
     CDS             complement(17006..17095)
                     /gene="cydH"
                     /locus_tag="ACHUPM_000973"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR0868829.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17394..18419
                     /gene="purR"
                     /locus_tag="ACHUPM_000974"
     CDS             17394..18419
                     /gene="purR"
                     /locus_tag="ACHUPM_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR0868830.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(18416..19348)
                     /gene="punR"
                     /locus_tag="ACHUPM_000975"
     CDS             complement(18416..19348)
                     /gene="punR"
                     /locus_tag="ACHUPM_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR0868831.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            19461..20672
                     /gene="punC"
                     /locus_tag="ACHUPM_000976"
     CDS             19461..20672
                     /gene="punC"
                     /locus_tag="ACHUPM_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR0868832.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            20963..22111
                     /gene="cfa"
                     /locus_tag="ACHUPM_000977"
     CDS             20963..22111
                     /gene="cfa"
                     /locus_tag="ACHUPM_000977"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR0868833.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(22151..22792)
                     /gene="ribE"
                     /locus_tag="ACHUPM_000978"
     CDS             complement(22151..22792)
                     /gene="ribE"
                     /locus_tag="ACHUPM_000978"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR0868834.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            23007..24380
                     /gene="mdtK"
                     /locus_tag="ACHUPM_000979"
     CDS             23007..24380
                     /gene="mdtK"
                     /locus_tag="ACHUPM_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR0868835.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(24421..25677)
                     /locus_tag="ACHUPM_000980"
     CDS             complement(24421..25677)
                     /locus_tag="ACHUPM_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR0868836.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            25985..26061
                     /locus_tag="ACHUPM_000981"
     tRNA            25985..26061
                     /locus_tag="ACHUPM_000981"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26019..26021,aa:Val,seq:gac)
     gene            26066..26142
                     /locus_tag="ACHUPM_000982"
     tRNA            26066..26142
                     /locus_tag="ACHUPM_000982"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26100..26102,aa:Val,seq:gac)
     gene            26250..26555
                     /locus_tag="ACHUPM_000983"
     CDS             26250..26555
                     /locus_tag="ACHUPM_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR0868837.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            26681..28285
                     /locus_tag="ACHUPM_000984"
     CDS             26681..28285
                     /locus_tag="ACHUPM_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFR0868838.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(28297..29109)
                     /gene="ydhT"
                     /locus_tag="ACHUPM_000985"
     CDS             complement(28297..29109)
                     /gene="ydhT"
                     /locus_tag="ACHUPM_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR0868839.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(29113..29898)
                     /gene="phsC"
                     /locus_tag="ACHUPM_000986"
     CDS             complement(29113..29898)
                     /gene="phsC"
                     /locus_tag="ACHUPM_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR0868840.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     VVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(29895..30563)
                     /locus_tag="ACHUPM_000987"
     CDS             complement(29895..30563)
                     /locus_tag="ACHUPM_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0868841.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(30627..31265)
                     /locus_tag="ACHUPM_000988"
     CDS             complement(30627..31265)
                     /locus_tag="ACHUPM_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR0868842.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(31278..>31869)
                     /locus_tag="ACHUPM_000989"
     CDS             complement(31278..>31869)
                     /locus_tag="ACHUPM_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HFR0868843.1"
                     /translation="GLPLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLR
                     VCLHNAVTLCNWVWPMTVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIF
                     TLHRAYTVKLMQTKDMRNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYK
                     EMGWDPQLGCPTRETLQRLGLEDIAADLAAHNLLPA"
BASE COUNT         7593 a         8386 c         7909 g         7981 t
ORIGIN      
        1 tttcaccctt tggcctgtaa ctcaatgata ttatgctcaa gttttgcgat gagcgtaatc
       61 agttgctcca gttcctctgc ggagatgcca tgtaatattt ccgcgcgggt tttgttaata
      121 acagcttcca tttcgctgat cagtggctct gccttttccg tcagtttaat acgtttagcc
      181 cgacgatcgc tggcacaagt ttgacgcgaa attaaccctt tttcttccag ttggtccaga
      241 gtacggacca gtgatggctg ctcgatgcca atcgcttttg ccagttgaat ttgcgactgg
      301 tctggaggta actgatggat attgtgtaac gtaacccaat gggtttgtgt taactccagc
      361 ggtttcaggc gatggtctat cagagcacgc catatgcgca ccaaccgtgc cagatcagaa
      421 cctagtggcg attccaattt catctcctta taattagctt gctaagatat tatgcggctt
      481 ttagaatagt gtgcagcaat tgtattgcta aaacaaatgt attgctgcat ttggttaccg
      541 tcagacatat ttttcagaaa ttgcgcgtaa atttttcgca cttaaagaat atttattaat
      601 ctaacgcaat atattcggtc gtaaaggaat ctactttgtg aagtttatgc tcaatgcaac
      661 aggattgccc ttacaagatc tggtgttcgg tgcgtccgtc tattttcctc cgtttttcaa
      721 agcattcgcg tttggattcg tcatctggct tgtcgtacac cgcctgcttc gtggctggat
      781 ctacgccggt gacatctggc atcccttgtt aatggattta tcgctgtttg cgatttgcgt
      841 ttgccttgct ctggcaatac tgattgcgtg gtaactatgt caattaaaac aattaagtat
      901 ttctcaacaa tcattgtagc ggtagttgcg gttcttgccg gatggtggct gtggaattat
      961 tacatgcaat ctccgtggac acgcgacggg aaaatacgcg cagaacaggt ttctatcacc
     1021 ccacaggtgt cgggacgtat tgttgagctg aatataaaag acaaccagct ggttaacgca
     1081 ggggatcttc tgctcacgat cgataaaacg ccctttcaga tcgccgagct gaacgcacag
     1141 gctcagctgg cgaaagcgca atctgacctc gcaaaagcca ataacgaagc gaatcgtcgt
     1201 cgtcatctct cacaaaattt tatttctgcc gaagaactcg ataccgctaa cctcaatgtt
     1261 aaagcgatgc aggccagtgt taatgccgca caggccacgc tgaaacagac acaatggcaa
     1321 ctggcgcaaa cggaaattcg cgccccggtg agtggacggg tgactaacct cactacccgc
     1381 atcggcgatt acgctgacac cgggaaaccg ctttttgccc ttgtcgacag ccactcgttt
     1441 tacgtcattg gttattttga agaaaccaag ttgcgccata tccgcgaagg tgcaccagca
     1501 caaattaccc tctatagtga taacaaaacg ttacagggtc acgtttccag tatcggtcgg
     1561 gcgatttatg atcagagcgt tgaaagtgac tcaagcctta ttccggatgt gaaacctaac
     1621 gtcccctggg ttcgtctcgc ccaacgcgtt cccgtccgct ttgcgctgga taaagttcca
     1681 ggcgatgtca cgctggtgtc gggcaccacc tgtagcattg ccgtaggtca ataatgaacg
     1741 catcgtaatg gtccttgcgc aatttgccct ggttcagggc cacgctggcg caatggcgtt
     1801 atgcgttacg caataccatt gccatgtgtc tggcgctgac ggttgcctat tatttgaatc
     1861 tggatgaacc ctattgggcg atgacctcgg ctgcagtggt tagctttccc accgttggcg
     1921 gtgtcatcag caaaagcctc ggacgcatcg ctggcagttt actgggagcc attgcggcac
     1981 tgcttcttgc cggacatacg ctcaatgagc cgtggttttt tctattgagc atgtcggcgt
     2041 ggcttggctt ttgtacctgg gcctgtgcgc acttcacgaa taacgtcgcg tatgcatttc
     2101 aactggcggg ctacacggct gccatcatcg cctttccgat ggttaatatt actgaggcca
     2161 gccagctgtg ggatatcgct caggcgcgcg tttgcgaggt gatagtcggt attttgtgcg
     2221 gcggcatgat gatgatgatc ctgcctagca gttccgatgc tactgccctt ttaactgcat
     2281 tgaaaaacat gcacgcccga ttactggaac atgccagttt actctggcag cctgaaacaa
     2341 acgatgccat tcgtgcagca catgaggggg tgattgggca gatactgacc atgaatttgc
     2401 tgcgtatcca ggctttctgg agccactatc gttttcgcca gcaaaacgcg cgccttaatg
     2461 cgctactcca ccagcaatta cgtatgacca gtgtcatctc cagcctgcga cgtatgttgc
     2521 tcaactggcc ctcaccgcca ggtgccacgc gagaaattct cgaacaattg ctgactgcgc
     2581 tcgccagttc gcaaacagat gcttacaccg tcgcacgtat tatcgccccg ctacgcccga
     2641 ccaacgtcgc cgactaccgg cacgtcgcct tctggcagcg actacgttat ttttgccgcc
     2701 tttatctgca aagtagtcag gaattacatc gtctgcaaag cggtgtagat gatcatgcca
     2761 gactcgcacg gacatccgct ctggctcgtc ataccgacaa cgccgaagct atgtggagcg
     2821 ggctgcgtac attttgtacg ttgatgatga ttggcgcatg gagtattgca tcgcaatggg
     2881 atgccggtgc caatgcatta acgctggcag caattagctg cgtactctac tccgccgtcg
     2941 cagcaccgtt taagtcgttg tcactgctga tgcgcacgct ggtgttactt tcgctattca
     3001 gctttgtggt caaatttggc ctgatggtcc agattagcga tctgtggcaa tttttactgt
     3061 ttctctttcc actgctggcg acaatgcagc ttcttaaatt gcagatgccc aaatttgccg
     3121 cactgtgggg gcaactgatt gtttttatgg gttcgtttat cgctgtcact aatcccccgg
     3181 tgtatgattt tgctgatttt cttaacgaga atctggcaaa aatcgttggc gttgcgctgg
     3241 cttggctagc gttcgccatt ctgcgtccag gatcggatgc tcgtaaaagc cgccgccata
     3301 ttcgcgcgct gcgccgggat tttgtcgatc agctaagccg ccatccaaca ctgagtggaa
     3361 gcgaatttga atcgctcact tatcatcacg tcagtcagtt gagtaacagc caggatgcgc
     3421 tggctcgccg ttggttatta cgctggggtg tagtgctgct gaactgttct catgttgtct
     3481 ggcaattgcg cgactgggaa tcgcgttccg atccgttatc gcgagtacgg gataactgta
     3541 tttcactgtt gcggggagtg atgagtgagc gtggcgttca gcaaaaatca ctggcggcca
     3601 cacttgaaga attacagcgg atttgcgaca gccttgcccg tcatcatcaa cctgccgccc
     3661 gtgagctggc ggcaattgtc tggcggctgt actgctcgct ttcgcaactt gagcaagcac
     3721 caccgcaagg tacgctggcc tcgtaattac ttaattacac cacaggcata gcgttcaccg
     3781 ccaccgccca gcggtttagg ttgatcggac atattatcgc cgccaacgtg gaccatcagc
     3841 gccttgtctt tgatttcatc cagtgatttc agacgaggcg cgatgacggc atcggtagct
     3901 ttgccgtcat tattgacgac cagtgcaggc agatcgccta aatgcccggt accttctggc
     3961 ccttcatgtt taccggtatt ttgtggatca agatgcccgc ctgcggattc cgcggcgctg
     4021 gctttgccat ctttggtggc tggctggcag cttcctttgg catgaatatg gaagccatgt
     4081 tcaccggggg gtaatgcttt cagatcgggt gaaaactcca gacctttatc ggtttcagta
     4141 atggtgacgc taccaattga ctgcccaacc ccttgcgacg tgacgaggtt catctcgact
     4201 ttttcactgg cagcttgtgc gccggtcgca acaaccagcg ccagaatggc cagactaaaa
     4261 cgtttcatag gacctccgtt cattgtgcat tcctgaataa gtgtaaacca gtgacacatt
     4321 tttgaacggc ggctattcct aaaagtgctt acggtacgtc gtaccccagt gccgctttac
     4381 ggatacgaaa ccattgttga cgggtcattt tcagtgtttc tgcttcgaca gctgcccgta
     4441 cgcgctcaat tttacctgag ccgataattg gcagcggctg cgacggtaaa cgtaataccc
     4501 aggcgtaaac cacctgttca atcgagcccg cgtttaactc ctccgccacc acagccagtt
     4561 catcacgcag cggctggaaa taatcatcat taaacagacg accaccacca aggcaggacc
     4621 acgccatcgg acgaacacgc agttgttgta gttggtcgag cgtgccatcc agcagtaacg
     4681 gctgatgcac cggggatatt tccacctgat tagtggcaag ggtaaacggc agacgtgatt
     4741 tcaacagggc aaattgcgca ggcgtaaagt tcgatacgcc aaaatgacgc actttgccgc
     4801 tctgatgcag atgtttgaac gcgtccgcca cttcatcggc atccattaac gggtctggtc
     4861 ggtggattaa cagcagatcc agatgatcgg tcgcgagatt aattagcgac tgttcggcgc
     4921 tcttaatgat gtgatcgcgg tcagtgatgt aatgaccaat gacgttttct tcacgcgcgg
     4981 tcgtcgcgat accgcattta ctgacgattt ccatccgttc acgcaggtga ggtgccagtt
     5041 tcagtgcctc gccaaacgcc gcttcgcact gatagccacc ataaatatca gcatggtcca
     5101 cggtggtcac gccgagatcc agatgctctt caataaaact gaccagctgg cgggcggaca
     5161 tattccagtc catcaatcgc cagtagccca tcacaaaacg ggaaaactcc gggccttgcg
     5221 gcgcaatagt aatacgctga accataatcg cttcctctta tcagatatga gaggagtata
     5281 cgcaagatta ggttcaaaag agtgatggtt gctccggttc gtctgatgac gctggcttat
     5341 ttgcgcgtaa tttgcgcatt aatcgctgcc gacaaaggcg cagcacctct tgtttttcgc
     5401 catcgctcat tttattccag ttaaaacgct catcccgact acgaaaacag ccgcgacaaa
     5461 acccgcgttc gtcagactgg caaattcccc ggcacgggct ctggacggga aagaactcta
     5521 attgctccgc cacttcgccc tcctcaaata agattattac cattattgaa gctgttaatg
     5581 tccaaagtag caactttgct tgcactagac cgactggtct actacactcc aacgcatgaa
     5641 caaacacacc gaacatgata ctcgcgaaca tctcctggcg acgggcgagc aactttgcct
     5701 gcaacgtggg ttcaccggga tggggctaag cgaattacta aaaatcgctg aagtgccgaa
     5761 agggtccttc tatcactact ttcgctctaa agaagcgttt ggcgttgcta tgcttgagcg
     5821 ccattacgcc acatatcacc agcgactgac tgagttgctg caatccggcg aaggtaacta
     5881 ccgcgaccgc atactggctt attaccagca aacactgaac cagttttgcc aacatggaac
     5941 catcagtggt tgcctgacag taaaactctc tgccgaagtg tgcgatctgt cagaagatat
     6001 gcgtagcgcg atggataaag gcgctcgcgg cgtgattgcc ctgctctctc aggcgctgga
     6061 aaatggccat gagaaccact gtttaacctt ttgtggcgaa ccgctgcaac aggcacaagt
     6121 gctttacgca ctatggctgg gtgcgaatct gcaggccaaa atttcgcgca attccgagcc
     6181 actggaaaac gcgctggcac atgtaaaaac cattattgcg acgcctgccg tttagcaggc
     6241 attttttatc accagacgac cgggagcctt tatgtcatct gaaaaactgt attccccact
     6301 gaaagtgggc gcaataactg cggcaaaccg tatttttatg gcaccgctga cgcgtctgcg
     6361 cagtattgaa ccgggtgaca tccctacccc gttgatggcg gaatactatc gccaacgtgc
     6421 cagtgccggt ttgattatta gcgaagccac gcaaatttct gcccaggcaa aagggtatgc
     6481 tggtgcacct ggcatccata gtccggaaca aattgccgca tggaaaaaaa ttaccgctgg
     6541 cgttcatgct gaaaatggtc atatggccgt acagctgtgg cacactggac gcatttctca
     6601 cgccagcctg caacctggcg gtcaggcacc ggtagcgcct tcagcactta gcgcgggaac
     6661 acgtacttct ctgcgcgatg aaaatggtca ggcgatccgt gttgaaacat ccatgccgcg
     6721 tgcgcttgaa ctggaagaga ttccaggtat cgtcaatgat ttccgtcagg ccattgctaa
     6781 cgcgcgtgaa gccggttttg atctggtaga gctccactct gctcacggtt atttgctgca
     6841 tcagttcctt tccccttctt caaaccatcg taccgatcag tacggcggca gcgtggaaaa
     6901 tcgcgcacgt ttggtactgg aagtggtcga tgccgggatt gaagaatggg gtgccgatcg
     6961 cattggcatt cgcgtttcgc caatcggtac tttccagaac acggataacg gcccgaatga
     7021 agaagccgat gcactgtatc tgattgaaca actgggtaaa cgcggcattg cttatctgca
     7081 tatgtcagaa ccagattggg cgggaggtga accgtatact gatgcgttcc gcgaaaaagt
     7141 acgcgcccgt ttccacggtc cgattatcgg cgcaggtgca tacacggtag aaaaagccga
     7201 aacgctgatt ggcaaagggt taattgatgc ggtggcattt ggtcgtgact ggattgcgaa
     7261 cccggatctg gtcgcccgct tgcagcgcag agctgagctt aacccacagc gtgccgaaag
     7321 tttctacggt ggcggcgcgg aaggctatac cgattacccg acgttgtaat ccaacattgc
     7381 gagcggcgta aagccgccgc tatactaaaa caacattttg aatctgttag ccattttgag
     7441 gataaaaaga tgcgtcttct tcataccatg ctgcgcgttg gcgatctgca acgctccatc
     7501 gatttttata ccaaagtgct gggcatgaaa ctgctgcgta ccagcgaaaa cccggaatac
     7561 aaatactcac tggcgtttgt tggctacggc ccggaaaccg aagaagcggt gattgaactg
     7621 acctacaact ggggcgtgga taaatacgaa ctcggcactg cttatggtca catcgcgctt
     7681 agcgtagata acgccgctga agcgtgcgaa aaaatccgtc aaaacggggg taacgtgacc
     7741 cgtgaagcgg gtccggtaaa aggcggtact acggttatcg cgtttgtgga agatccggac
     7801 ggttacaaaa ttgagttaat cgaagagaaa gacgccggtc gcggtctggg caactaatct
     7861 cctgccgggc gtgaactcat cgcgcccgca tctttactgc atcgacaagt aatatttgtc
     7921 ataatgcgcg ctgcaattta tccgtattaa gagaatcaga tgtccgataa cgctcaactt
     7981 accggtctgt gcgaccgttt tcgtggtttt tatcctgttg tgatcgatgt tgaaacagcc
     8041 ggatttaacg ccaaaaccga tgcgctgctt gagattgccg ccatcaccct gaaaatggat
     8101 gaacaaggct ggctgatgcc ggacaccaca ttacatttcc acgtcgaacc atttgtcggc
     8161 gcaaatttgc agccagaggc cctcgccttc aacggcattg acccgaacga tcccgatcgc
     8221 ggcgcggtca gcgaatacga ggcgctgcac gaaattttta aagttgtacg taaaggtatt
     8281 aaagcgagcg gctgtaaccg cgccattatg gtggcgcaca atgccaattt tgatcacagc
     8341 tttatgatgg ccgccgcaga acgcgcctca ctgaaacgta acccgttcca ccctttcgcc
     8401 acttttgaca ctgctgcact ggccgggctg gcactcggac aaaccgtatt gtcaaaggct
     8461 tgccagaccg ctggcatgga cttcgacagc acccaggcgc actccgcgct gtacgacacc
     8521 gaacgcactg ctgtgctgtt ttgtgaaatc gtcaaccgct ggaaacgtct gggaggctgg
     8581 ccgctacctg ccgccgaaga ggtgtaatcg agtcgatgcc tgatgacatg caatgattca
     8641 ggcatctata gtgaggctat tccacgcatc aagcatgata ttcacgggga atagcgttaa
     8701 tggcagataa tccagaccct tcatcgctcc tgccggacgt gttttcactg gcgacccgcg
     8761 actggtttct tcgcgccttt aaacagccga ccgctgtcca gtcgcaaacc tggcatgtgg
     8821 cggcgcgagg cgaacatgcg ctggtgattg caccgactgg ctccgggaaa acgctggcag
     8881 catttctcta cgccctcgat cggctcttcc gcgaaggcgg cgaagatacc cgcgaggcgc
     8941 ataagcgtaa aacctcacgc atcctctata tttcaccgat aaaagccctg ggcaccgacg
     9001 ttcagcgcaa cttgcagatc ccgttgaaag gtattgccga tgaacggcgg cggcgcggcg
     9061 aaacggaagt caatcttcgc gtagggatcc gtactggcga tacgcctgca caggaacgca
     9121 gcaaactcac ccgtaatccg ccggatattc tgatcaccac acccgaatca ctctatctga
     9181 tgctgacctc ccgcgcgcgc gaaacgctac gcggcgtcga aacggtaatt attgatgaag
     9241 tccacgcggt agcgggcagt aaacgtggtg cgcatctggc gttaagtctg gagcggctcg
     9301 atgcgctgct ccacacctca gcacagcgaa ttggcctttc tgccactgtg cgctcagcca
     9361 gcgatgtggc agcatttctt ggtggcaatc gcccggttac ggtagtcaac ccgcccgcaa
     9421 tgcgccatcc gcagatacga attgtcgtac cggtcgccaa tatggatgat gtctcatcgg
     9481 tcgccagcag caccggcgaa gacagccatg ccggccggga aggctccatc tggccatata
     9541 ttgaaacggg tatccttgat gaagtgttgc gccatcgctc gaccattgtc tttactaatt
     9601 cacgtgggct ggcggaaaaa ctgacggcac gattaaatga gctttacgcc gcacgcttac
     9661 agcgttcccc gtctatcgcc gttgatgcgg cccatttcga gtcgacctcc ggcgcaacct
     9721 ctaaccgtgt acaaagtagc gacgttttta ttgcccgctc acaccacggc tccgtctcta
     9781 aagaacaacg agcaatcacc gagcaggcgc tgaaatcggg tgagttacgc tgcgtggtcg
     9841 caacctccag tctcgaactg gggattgata tgggcgcagt ggatctgatg attcaggtgg
     9901 caacgccgct ttctgttgcc agtgggttac aacgcattgg tcgcgccgga catcaggttg
     9961 gcggtgtatc taaagggctg tttttccctc gtacccggcg tgacttagtc gattccgcag
    10021 tcattgtaga gtgtatgttc gcaggcaggc tggaaaacct gacaccgccg cataatccac
    10081 tcgacgtcct tgcgcagcaa accgttgccg ccgcggcgat ggatgcatta caggtagacg
    10141 aatggtactc ccgcgtacgc cgtgccgcac cgtggaaaga tctgccaaga cgtgtttttg
    10201 acgctacgct ggatatgctt tccgggcgct atccctctgg cgatttttct gcttttcgcc
    10261 ccaaactggt ctggaaccgg gagaccggga tattgaccgc ccgacctggc gctcaattgt
    10321 tggcggttac cagcggtagc accattccgg atcgtggcat gtatagcgtg ttattacccg
    10381 aaggtgaaga acaggccggt tcgcggcggg tgggtgaact ggatgaggag atggtatatg
    10441 agtcgcgggt gaacgacatt atcactctcg gcgctacctc atggcggatc cagcaaatca
    10501 cccgcgatca ggtgattgtg actcctgctc cgggtcgttc tgcccggctc cccttctggc
    10561 gtggtgaagg taacggacgt ccggctgaat taggcgagat gatcggcgat tttcttcatt
    10621 tgctggcgga tggcgcgttc ttttccggga ctattccccc gtggctggca gaagaaaata
    10681 cgatcgccaa tattcagggg ttgattgacg agcaacgcaa cgcgacgggc atcgttccgg
    10741 ggagtcgcca tctggtcctc gaacggtgcc gtgatgaaat tggcgactgg cgtattattt
    10801 tgcactctcc ctatggaaga cgggtgcatg aaccctgggc gctggcgatt gccgggcgaa
    10861 tacatgccct atggggcgct gacgcgtctg tggtcgccag tgatgacggc attgttgcgc
    10921 gcattcctga cactgatggt aaattgcccg acgccgcgat ttttttgttt gaaccagaaa
    10981 agttgctgca aattgtccgc gaggcggtag gcagctcggc acttttcgcc gcccgttttc
    11041 gcgaatgcgc cgcgcgggca ttattaatgc cggggcgcac tccgggccat cgcaccccgc
    11101 tttggcaaca acggctgcgc gccagtcagt tgctggaaat cgctcaggga tatccggatt
    11161 ttccggtcat tctcgaaacc ctacgcgaat gtctgcaaga tgtttatgat cttcccgcac
    11221 tggaacgttt gatgcgtcgc ctgaacggtg gcgaaattca aatatccgat gtaacgacca
    11281 ctacgccctc gcctttcgcc acaagtttat tgttcggcta tgtcgcggaa tttatgtacc
    11341 agagcgacgc cccgctggca gagcgccggg catccgtact gtcgctggac agcgagttac
    11401 tgcgcaatct actcggacag gtcgatccgg gggaattact cgacccgcag gtcattcgcc
    11461 aggtggaaga agagttgcaa cgactggctc ctggcagaag agcgaaaggt gaagaaggat
    11521 tgttcgacct gctgcgcgaa ctggggccaa tgaccgttga agacctggcg caacggcata
    11581 caggcagcag tgaagaggtt gcgtcgtatc tggaaaatct tcttgcagta aaacgaatct
    11641 tcccagcgat gattagcgga caggagcgtc ttgcctgtat ggatgatgcc gccaggctgc
    11701 gtgatgccct cggcgtacga ctaccagagt cattgccaga gatttattta catagagtca
    11761 gttacccgct tcgcgacctc tttctgcgct atctccgggc tcatgctctg gtcacggctg
    11821 aacaactggc tcatgagttt agtctcggta ttgccattgt cgaagagcag cttcagcaac
    11881 tgcgtgaaca gggtctggtg atgaatctgc aacaagacat ctgggtgagc gatgaagtat
    11941 ttcgtcgtct gcgtttgcgc tcgctgcaag ccgccagaga agcgacgcgt cccgttgcag
    12001 ccacgaccta tgcgcgattg ctgctggaac gtcagggcgt attacccgcc accgatggta
    12061 gcccggcgct ctttgcctca acatcgccag gcgtttatga gggcgtagat ggcgtgatgc
    12121 gggtgatcga acagcttgcc ggagtcggtt tacccgcctc actctgggaa agccagatcc
    12181 tgcctgcccg cgtacgcgac tattcgtcag aaatgctcga tgaattactg gcaaccggtg
    12241 cggttatctg gtcggggcaa aaaaactggg tgaagatgac ggcctggtgg cactgcatct
    12301 acaggaatat gctgcagaat cgttcactcc cgccgaagcg gatcaggcga atcgttcggc
    12361 gctgcaacaa gcgatagtcg ctgttctggc tgacggagga gcctggtttg cacaacaaat
    12421 cagccaacgg atacgcgaca aaatcggcga atcggttgat ctctctgccc tgcaagaggc
    12481 gttatgggcg ctggtctggc aaggcgtcat caccagcgac atttgggcac cgttacgcgc
    12541 cctcacccgc agcagttcca acgcacgcac ctcaactcgc cgcagtcacc gggctcgtcg
    12601 tggacgtcct gtctatgcgc aacccgtctc gccgctggta tcttacaaca caccaaatct
    12661 ggctggatgc tggcgctggc ggaaaatatg ctcgaccgct acggcatcat cagtcgtcag
    12721 gcggtgatag ccgaaaatat ccctggcggg tttccatcga tgcaaacgct ttgtcgaagt
    12781 atggaagact ccgggcgaat tatgcaaggt cgttttgtag aaggtctggg tggcgcgcaa
    12841 ttcgctgaac gtctgactat tgaccgattg cgcgatctgg cgacacaagc cacgcaaacg
    12901 cgccactata caccagtggc gctctctgcc aacgatccgg ctaatgtgtg gggaaatctt
    12961 ctgccctggc ctgcacatcc ggcaacgctg gttccaacgc gtcgggcggg tgcgctggtg
    13021 gtcgtttctg gcggcaaatt gttactctat ctggcgcaag gtggcaaaaa aatgctggtc
    13081 tggcaggaaa aagaggaatt actcgcccca gaggttttcc acgcgctgac taccgcactg
    13141 cgtcgcgaac cacggctgcg ctttacgcta acagaagtga atgatctacc ggtccggcaa
    13201 acgccgatgt ttacgctgct gcgtgaggcg ggattttcaa gttcgccaca agggctggat
    13261 tggggataga gaaaggactg acggatgccc gttcgcatcc gtcagtattg caggacggat
    13321 tattccgcgt ccggctcttc agacttgtat ttagcggcag tttctttgat cagctgctgc
    13381 agttcgccac gctgatacat ttcgatcacg atatcacaac cgccgaccag cttgccgtca
    13441 acccacagtt gcgggaaggt cggccagtta gcatatttcg gcagttccgc acgaatgtcc
    13501 ggattctgca gaatatcaac ataggcaaaa cgttcgccac atgcggcaag cgcctggact
    13561 gcctgggcag agaaaccgca gctcggcagt ttcggtgaac ctttcatgta cagcaggatc
    13621 gggttttcag caatctggcg ttggattttt tcgatagtgg tgctcattgt cttgcttcct
    13681 caaacttctt tacggcagta atctgacatt gtagcgggtc agtgcggcat cggaaaataa
    13741 cattttcatc acgcttttgc tattttatcc ctttgctcta tttgttgcat ttcaaatatt
    13801 cgtttcttct ctgatgccgg caaactggcg tatttataac catttttagc tttcactgct
    13861 gcattttttt gcgctcgcca acgaaacgta ttttttaaca ataaaagcta ttaactttct
    13921 cttcttctat gcattagaat catcaagttt tgtaaatcag acgcaggcat gatagacctg
    13981 ccttaacaga gggacgctca gtggcgcgga taaaccgtat ttcgatcacg ctctgtgctt
    14041 tactttttac caccctgcct ttaacgccta tggcccatgc ttcaaagcaa gccagggaga
    14101 gttctgctac cactcatatc accaaaaaag cagataaaaa gaaaagcacg gcaaccacca
    14161 aaaagaccca gaaaacagcg aaaaaagccg ccagtaaaag tacgaccaaa agcaaaaccg
    14221 cttcttccgt taaaaaatct tccattaccg cttctaaaaa cgccaaaact cgcagcaaac
    14281 acgccgtcaa taaaacggcc tcagccagct ttaccgaaaa gtgtaccaaa cgtaagggct
    14341 ataaatcgca ttgtgtgaaa gtcaaaaatg ccgcgtcagg aactcttgcc gacgcgcaca
    14401 aagcgaaggt gcaaaaagct acgaaagtgg caatgaataa actgatgcag caaattggta
    14461 agccatatcg ttggggtggc agctcaccgc gtaccggttt tgattgcagc ggcctggttt
    14521 attacgctta taaagatttg gtgaaaattc gtattccgcg tacggcgaat gaaatgtatc
    14581 acctgcgtga tgcagcgcca atcgaacgta gtgaactgaa aaacggcgac ctggtctttt
    14641 tccgtactca gggacgcggc acagccgatc atgtcggcgt gtatgtcggc aacggcaaat
    14701 ttattcagtc accgcgcaca ggtcaggaaa ttcaaatcac ttctctcagt gaagactact
    14761 ggcagcgcca ctatgttggc gctcgtcggg taatgacccc aaaaacactt cgctaaaact
    14821 ttaccctgtt gttacggcaa cagggtaagt tcatcttttg tctcaccttt taatttgcta
    14881 ccctatccat acgcacaata aggctattgt acgtatgcaa attaataata aaggagagta
    14941 gcaatgtcat tcgaattacc tgcactacca tatgctaaag atgctctggc accgcacatt
    15001 tctgcggaaa ctatcgagta tcactacggc aagcatcatc agacttatgt cactaacctg
    15061 aacaacctga ttaaaggtac cgcgtttgaa ggtaaatcac tggaagagat tattcgcagc
    15121 tctgaaggtg gcgtattcaa caacgcagct caggtctgga accatacttt ctactggaac
    15181 tgcctggcac cgaacgccgg tggcgaaccg actggaaaag tcgctgaagc tatcgccgca
    15241 tcttttggca gctttgccga tttcaaagcg cagtttactg atgcagcgat caaaaacttt
    15301 ggttctggct ggacctggct ggtgaaaaac agcgatggca aactggctat cgtttcaacc
    15361 tctaacgcgg gtactccgct gaccaccgat gcgactccgc tgctgaccgt tgatgtctgg
    15421 gaacacgctt attacatcga ctatcgcaat gcacgtcctg gctatctgga gcacttctgg
    15481 gcgctggtga actgggaatt cgtagcgaaa aatctcgctg cataataact gatggcaaat
    15541 gcagcattgc ctgatgcgct tcgcttacca tgccaacgcg gatgaggcgt taacagcgca
    15601 ttcggcaaga taaatcgcac gttgtcagca actgtaacgc agaaggttat ccttctgcgt
    15661 ttttgtttaa ttagctgttg gcaacgcaaa ctgtttcagg ttgttttctg gctgacataa
    15721 acaccagcaa taatgccagt cccgcgacaa tcgcccccat caccggcaca aagctgtatc
    15781 ccagcccagc ggaaattacc gcaccaccag ccgctgctcc cagcgcattt ccaagattaa
    15841 aggcaccaat attgactgat gaagacagac ctggcgcttc actggcgaca cgcatcacgc
    15901 gcatctgtaa cggcggtacg accgcaaagg ttgctgcgcc ccacaccacc atgctaatag
    15961 ctgcgccgaa ctcattgcgg gccaggaacg gggttgccag cataatcacc atcagcagca
    16021 acaaaaagcc tttcaacgtg ccgttaactg aacgatctgc cagtttgccg ccgagatagt
    16081 taccgataga gaatccgaca ccaatcagca ccagcattgc cgtgacgaac accggtgttg
    16141 cgtgggtaat actttgcagt accggagaga tataggtgta gagagtaaac attgcgccag
    16201 ctcccagtac cgtcgtcagc aatgcagaca gcacctgcgg acgcattaat accgccagct
    16261 cttttttcac ttcaggtcgt gcccctgtac cacctttagg taatgagaag aacagactta
    16321 ccattgaaat cactcccagc cccgccgttg ccagaaatga catccgccag ccgatggttt
    16381 cacccaacca ggtcgccgcc ggcacgccac cgatatttgc cagggttaac cccataaaca
    16441 tagtggcaac tgcgctggcc tgtttatgtt ttggcaccac gcttgcggcc acgactgaac
    16501 ccaaaccaaa aaatgctccg tgattcaggc tggtcaaaat gcgtgaaagc atcagggtca
    16561 tataatccgg cgcgatggcg gaaagtacgt tgccgagcgt gaaaattgcc atcaggaaaa
    16621 tcaacgcact gcggcgggca cgatgagaaa gtagaagcgt catcagcggc gcgcccacca
    16681 ttacgccaac tgcataggca ctgattaaca ttccggcagc gggaatcgag acatccacac
    16741 cgcgcgcaat gacgggcaac aagcccattg gcgagaactc cgttgtcccg ataccaaacg
    16801 cgccaatcgc cagcgccagc aacggatagt taattttcat gccttatctc cacctcttcg
    16861 cgtcattacg cgatattcat taaagtggcg aaagcatgac agcaatcaca aaaaaatgaa
    16921 aataacaaaa agagaaaaca cttttgccat tttgctaaca aacaggaagg agatgcgagg
    16981 gagaacgcgc tccctcgaga ggaaatcagt gcagcgcggc agtcaaaccc acggctacga
    17041 tcaaaccgag gacgataatc gttgttacca gtgaaaattt aaggtcggtg ctcatcaagt
    17101 tttctccttt tttattacca cacaaaaagt gatattacgc atttttacac actgtgatga
    17161 aaaaatctcc cgtcatttat aatgataagt gtttttacca cttccccttt tcgtcaagat
    17221 cggccaaaat tccacgctta cactatttgc gtactggcca ttgacccctt cctgacgctc
    17281 cgtgtcgttt ttccggcgta ccgcaacact tttgttgtgc gtaaggtgtg taaaggcaaa
    17341 cgtttacctt gcgattttgc aggagctgaa gttagggtct ggagtgaaat ggaatggcaa
    17401 caataaaaga tgtagcgaaa cgagcaaacg tttccactac aactgtgtca cacgtgatca
    17461 acaaaacacg tttcgtcgct gaagaaacgc gcaacgccgt gtgggcagcg attaaagaat
    17521 tacactactc ccctagcgcg gtggcgcgta gcctgaaggt taaccacacc aagtctatcg
    17581 gtttgctggc gaccagcagc gaagcggcct attttgccga gatcattgaa gcagttgaaa
    17641 aaaattgctt ccagaaaggt tataccctga ttctgggcaa tgcgtggaac aatcttgaga
    17701 aacagcgggc ttatctgtcg atgatggcgc aaaaacgcgt cgatggtctg ctggtgatgt
    17761 gttctgagta cccagagccg ttgctggcga tgctggaaga gtatcgccat atcccaatgg
    17821 tggtgatgga ctggggtgaa gcaaaagctg acttcaccga tgcggtcatt gataacgcgt
    17881 tcgaaggcgg ctacatggcc gggcgttatc tgattgaacg cggtcaccgc gaaatcggcg
    17941 ttatccccgg cccgctggaa cgtaacactg gcgcaggccg ccttgtcggt tttatgaagg
    18001 cgatggaaga agcaatgatc aaggtgccgg aaagctggat tgtgcagggt gactttgaac
    18061 ctgaatccgg ttatcgcgcc atgcagcaaa tcctgtcgca gccgcatcgc cctactgccg
    18121 tcttctgtgg tggcgatatc atggcaatgg gcgcactttg tgctgctgat gaaatggggc
    18181 tgcgcgtccc gcaggatgtt tcgctaattg gttatgataa cgtgcgcaac gcccgctatt
    18241 ttacgccggc gctgaccaca atccatcagc caaaagattc gctgggtgaa acagcgttca
    18301 acatgctgtt ggatcgtatc gtcaacaaac gtgaagaacc gcagtccatt gaagtgcatc
    18361 cgcgcttgat tgaacgccgc tccgtggctg acggcccgtt ccgcgactat cgtcgttaat
    18421 cacccgttgc gggagtctct tccggctccc gcagccactc cttattcagc gtctcactat
    18481 cgccgagata ctcaagcaac caggttaacg caggcgacat atcattttgc tgccatgtca
    18541 gacaacatgc cgaatccgga aaggggtttt ccagttcaag tgctacccac ttcccctcat
    18601 taagccacgg tttggcgaaa tgtgttggca ccatccctat gcataatcct gccgagatac
    18661 aggttgccga tgattcccag tcaggcacga caactctttt ttggttatcc agcaaccagg
    18721 taatacgttt aggtagcgtt cgcgaggtgt cttcgcgcac caacgacggc cagttgcgca
    18781 acgtatcatc gctgaacggg ccatccatca acgccagcgg gtgatggctg gcaacaacgc
    18841 aactccagct tagcatcccc atatcccgga aggcataacg accgcctacc ggaatcgcgc
    18901 gtgttgcgcc aatcgccagt tccacgcgcc cgtcggaaag cgcatcccag acaccgttga
    18961 acacttcctg aaagacaaga agttcgacat catcaaaatg gcgataaaaa tcaacgatca
    19021 tctgccgtgt acgttctggc ctgacaatat tatccactgc gatagctaac tgaccgcgcc
    19081 agccgttcgc tatctgctga cattgctggc gggtgatctg catttttttg acaacagagc
    19141 gcccttcttt gagaaaccac gctccagcag cggtcagctc cacatcacgg tgccgtcgtt
    19201 caaagagcgg caccgccagc cactcttcca gctgacgcac ggtatagctg accgcagaag
    19261 gaacgcgatg cagctcctgt gccgcagcgc taaaactacc attacgcgct accgcatcaa
    19321 caacttcgag tgaatattct gaccacatag tctgcctgca aaatttttga aaccaatcat
    19381 caaatattac cgtttcacaa cactaatttc actccctaca ctttgcggcg gtgtttaatt
    19441 gagagattta gagaatatac atgcaacctg ggaaaagatt tttagtctgg ctggcgggtt
    19501 tgagcgtact cggttttctg gcaaccgata tgtatctgcc tgctttcgcc gccatacagg
    19561 ccgacctgca aacgcctgcg tctgctgtca gtgccagcct tagtctgttc cttgccggtt
    19621 ttgccgcagc ccagcttctg tgggggccgc tctccgaccg ttatggtcgt aaaccggtat
    19681 tattaatcgg tatgacaatt tttgcgttag gtagtctggg gatgctgtgg gtagaaaacg
    19741 ccgctacgct gctggtattg cgttttgtac aggctgtggg tgtctgcgcc gcggcggtta
    19801 tctggcaagc gttagtgacg gattattatc cttcacagaa agttaaccga atttttgcga
    19861 ccatcatacc gctggtgggt ctatctccgg ccctggctcc tctgttagga agctggctgc
    19921 tggtccattt ttcctggcag gcgattttcg ccaccctgtt tgccattacc gtggtgctga
    19981 ttctgcctat tttctggctc aaacccacga cgaaggcccg taacaatagt caggatggtc
    20041 tgacctttac cgacctgcta cgttctaaaa cctatcgcgg caacgtgctg atatacgcgg
    20101 cctgttcagc cagttttttt gcatggctga ccggttcacc gttcatcctt agtgaaatgg
    20161 gctacagccc ggcagttatt ggtttaagtt atgtcccgca aactatcgcg tttctgattg
    20221 gtggttatgg ctgtcgcgcc gcgctgcaga aatggcaagg caagcagtta ttaccgtggt
    20281 tgctggtgct gtttgctgtc agcgtcattg cgacctgggc tgcgggcttc attagccatg
    20341 tgtcgctggt cgaaatcctg atcccattct gtgtgatggc gattgccaat ggcgcgatct
    20401 accctattgt tgtcgcccag gcgctgcgtc ccttcccaca cgcaactggt cgcgccgcag
    20461 cgttgcagaa cactctacaa ctgggtctgt gcttcctcgc aagtctggta gtttcctggc
    20521 ttattagtat cagcacgcca ttgctcacca ccaccagcgt gatgttatca acagtagtgc
    20581 tggtcgcgct gggttacatg atgcaacgtt gtgaagaagc tggctgccag aatcatggca
    20641 atgccgaagt cgctcatagc gaatcacact gatctatatc gatatactta tacttaggct
    20701 gctaacaaaa ttttgttgta tgattgaaat tagcggccta tactaatttc gagttgttaa
    20761 agctacgata aatattatgt ttttacgggg acaggatcgt tcccgactca ctatggatgg
    20821 tcatttcggc aagggttcct cctttccctc tgttctacgt cggattatag actcgcggtt
    20881 ttttctgcga gatttctcac aaagcccaaa aagcgtctac gctgttttaa ggttctgatc
    20941 accgaccagt gatggagaaa ctatgagttc atcgtgtata gaagaagtca gtgtaccgga
    21001 tgacaactgg taccgtatcg ccaacgaatt acttagccgt gccggtatag ccattaacgg
    21061 ttctgccccg gcggatattc gtgtgaaaaa ccccgatttt tttaaacgcg ttctgcaaga
    21121 aggctctttg gggttaggcg aaagttatat ggatggctgg tgggaatgtg accgactgga
    21181 tatgtttttt agcaaagtct tacgcgcagg tctcgagaac caactccccc atcatttcaa
    21241 agacacgctg cgtattgccg gcgctcgtct cttcaatctg cagagtaaaa aacgtgcctg
    21301 gatagtcggc aaggagcatt acgatttggg taatgacttg ttcagccgca tgcttgatcc
    21361 cttcatgcaa tattcctgcg cttactggaa agatgccgat aatctggaat ctgcccagca
    21421 ggcgaagctc aaaatgattt gtgaaaaatt gcagttaaaa cctgggatgc gcgtactgga
    21481 tattggctgc ggctggggcg gactggcaca ctacatggca tctaattatg acgtaagcgt
    21541 ggtgggcgtc accatttctg ccgaacagca aaaaatggct caggaacgct gtgaaggcct
    21601 ggatgtcacc attttgctgc aagattatcg tgacctgaac gaccagtttg atcgtattgt
    21661 ttctgtgggg atgttcgagc acgtcggacc gaaaaattac gatacctatt ttgcggtggt
    21721 ggatcgtaat ttgaaaccgg aaggcatatt cctgctccat actatcggtt cgaaaaaaac
    21781 cgatctgaat gttgatccct ggattaataa atatattttt ccgaacggtt gcctgccctc
    21841 tgtacgccag attgctcagt ccagcgaacc ccactttgtg atggaagact ggcataactt
    21901 cggtgctgat tacgatacta cgttgatggc gtggtatgaa cgattcctcg ccgcatggcc
    21961 agaaattgcg gataactaca gtgaacgctt taaacggatg tttacctatt atctgaatgc
    22021 ctgcgcaggt gctttccgcg cccgtgatat tcagctctgg caggtcgtgt tctcacgcgg
    22081 tgttgaaaac ggccttcgag tcgctcgcta aaggctattc tatcgccccc tctccggggg
    22141 cgatttcaga tcaggcttct gtacctggtt gattcatggc attttctcgt gccgccagca
    22201 cacgttctac cgtatctacc actgcctgag tttgtggatc aatttcaatg ttgacgcgtg
    22261 cgccgagttt tttcttccca agagtcgtgc gttccagagt ttccggaatt aaatggacgc
    22321 aaaaacgcgt tggcgtgact tcgccgacgg tcaggctaat accgtcgatg ccaataaatc
    22381 ctttgtacag gatatatttc atcaactgac tatcctggac tttaaaccag atctggcgat
    22441 tattttccga ggttaatatt ttcgccactt cagcggtggt cataatatga cctgacatta
    22501 agtgtccgcc aatttcatcg ctgaattttg ccgcacgctc aacgtttacc caatccccca
    22561 cttttaaatc gccaagattg gtaatgcgta acgtttcttt catcaggtca aaactgacat
    22621 ggttgccgtt aatttccgtc actgtcaggc agcaaccgtt atgcgccacg gaagcacctg
    22681 tttccaggcc gtccagcatg tggtcgggta actccaccac atgcgtacga aaatttggtt
    22741 tctcgtcaat cgacaccagt tttgcggtgc cctgtacaat ccccgtaaac atacttacaa
    22801 ctcctgaaat cagttaagac attctgttca gcacaatagc aggtggaaaa cgcccttacc
    22861 agtgaagggg taagaatgac tattttttca ctggagaatt aataaatcct cgctacaata
    22921 gactgaattt cccctgcttc ttctttttgc tgcccattca ggcggctttt tagtctctca
    22981 tataactaca aataaaaggt gttcacgtgc agaagtatat cagtgaagcg cgtctgttat
    23041 tagcattagc aatcccggtg attctcgcgc aaatcgccca aactgcgatg ggttttgtcg
    23101 ataccgtgat ggcgggcggc tatagtgcca ccgacatggc ggcggtcgct atcggtactt
    23161 ctatctggct tccggcgatc ctctttggtc acggactgct gctggcatta acgccggtta
    23221 tcgcgcaatt aaatggttcc ggtcgacgtg agcgcattgc gcatcaggtg cgacaaggtt
    23281 tctggctggc aggttttgtt tccgttctca ttatgctggt gctgtggaat gcaggttaca
    23341 ttatccgctc catggaaaac atcgatccgg ctctggcgga caaagccgtg ggttatctgc
    23401 gtgcgttgtt gtggggcgcg ccaggctatc ttttcttcca ggttgcccgt aaccagtgtg
    23461 aaggtctggc aaaaaccaag ccgggtatgg taatgggctt tatcggcctg ctggtgaaca
    23521 ttccggtgaa ctatatcttt atttatggtc atttcggtat gcctgagctc ggtggcgttg
    23581 gttgtggcgt ggctactgcg gcggtgtatt gggttatgtt ccttgccatg gtttcttaca
    23641 ttaaacgcgc ccgctccatg cgcgatatcc gtaacgaaaa aggcaccgca aaacccgatc
    23701 ctgcggttat gaaacgactg attcaactcg gtttgccgat tgcgctggca ctattctttg
    23761 aagtgacact gtttgccgtc gtggctctgt tagtgtctcc gctcggtatt gttgatgtcg
    23821 caggacacca gattgccctg aactttagtt cactaatgtt cgtgcttcca atgtcgctgg
    23881 cggcagcggt aactatccgc gtaggttatc gtctgggtca gggctcaacg ctggatgcgc
    23941 aaaccgctgc tcggaccggg cttatggtgg gtgtctgtat ggcaaccctg acggccattt
    24001 tcacggtttc actgcgggag caaatcgccc tgttgtacaa cgacaatccc gaggttgtaa
    24061 cgctggctgc gcatttgatg ttgctggcgg cggtatatca gatttctgac tcaatccagg
    24121 tgattggcag tgggattttg cgtggttata aagatacgcg ttccattttc tatatcacct
    24181 ttacagctta ctgggtgctg ggcttgccaa gcggctatat tctggcactg accgatctgg
    24241 tcgttgaacc tatggggcca gcaggcttct ggataggctt tattatcggc ctgacatcgg
    24301 cagccattat gatgatgctg cgtatgcggt tcctgcaacg tatgccgtca gccatcattc
    24361 tgcaacgagc gtcccgctaa taaagacaag gcgcaacctt cacgggttgc gcctgtgttt
    24421 ttacgcaggc tggagcgttg cgccaatccc gtcttcgtct ggctgtaatt tcagagcgtt
    24481 acacagagtt tgccgactgg catctgttat ctcaacgcgt atatccccgt taacttcccc
    24541 tttcacattg atgaagtcag gtgaaatttc tttcgcactg gtttgcaggc aaatcgctcc
    24601 ctctccgcgc aggcgtttca ctttcagttg cacatgcgtt gccgcaccag tgatcagcca
    24661 cagttttccg ccattcagat tgatgttacc aacaatgaaa gtgacgctgt caccttttac
    24721 cgtactgccg tcgagaatca acacaccgcc ctgctgtaca gtaatcgttg attgttgcag
    24781 ctctgcgcca taaccaataa tcattgaagc gccgtccagt aatgcaacac tcccggtcag
    24841 ttcaaccggt gcgcgatctt cctctacgac aaaattttcg tccgcgctgg tgggtgacaa
    24901 caggatcact gttccacctt gtattaccgc attttccagc gtaccggcat gcacttcagc
    24961 ccgccccccc acattcaccg acaggttttg cgtcttaccg gaactgtaga gctgaaggcc
    25021 atccgtcccc agacgataaa tggatccatt ctcaacaata gtgtcagagt cttcaccacc
    25081 gagattattc atggtgccac cagcaagcaa cgtatcactg gctttcccgg ccagcataaa
    25141 aacgccgccc tgattaatct cacaattgct ggcttctcca cttggataca ccaacaattc
    25201 accaccttca tctaccacga ccgctgaggt tgtcccatta accaacaggc cgccctcttg
    25261 atcgagaatg attttttccg cgcgatgtcc ttccagtaca cgcaggttac cgccattttc
    25321 cagcaacaaa ccgcaggcat aaccctgatc aacgctgaat tcaccttcgg gatggcggcc
    25381 attaaccgta gcgagcgtag agagactaat tgcgcctccc tcggactgaa caatatctgt
    25441 ggcaatggca ccgtcgctga ccgatagcgt gccattgtta ccgtactgtg tgcatttggc
    25501 gatcccaccg gttgccactt tcaaccagcc agcccccgtg accacggtat tttcggctct
    25561 tccgccagct tcaacaatca actcgccttc agtaagttgc gtcgcgccaa tgacctcgcc
    25621 ggtcttaaca atttgcacat tcccgtcgct catcaagttt ttcgcatcag atcccatgat
    25681 ttattccttt gctgcatctg tgtgccttta ttgctaccta agtgtaaagg ctacggagga
    25741 tttatccacg acagatttga gatggtggca aacaattctg tttaaactct gatacacgaa
    25801 ttattgggtt gtatcagatg taaatgcgat cctgaataaa aatcgccctt gcaaatcaac
    25861 aaaatatagc caatttgctt aaaagctctg caaacggtcg aaatagcgca gaaaattacg
    25921 ttttgcctct tgccaccttt ccactctgcc gctaatattt gtccccgttg tcacctacaa
    25981 cgttgcgttc atagctcagt tggttagagc accaccttga catggtgggg gtcgttggtt
    26041 cgagtccaat tgaacgcacc atcctgcgtc cgtagctcag ttggttagag caccaccttg
    26101 acatggtggg ggtcggtggt tcgagtccac tcggacgcac cagattttct taatctggtc
    26161 ttttcctttt tccctctgtt tattctctgt atccaatacg ttaaaagatt tacactgtct
    26221 tcgtatgcgt tatcagaagg agaatcgcta tggcaacttt gttacaactt cattttgctt
    26281 ttaatggccc atttggcgac gcaatggctg agcagcttaa accacttgct gagtcgatta
    26341 atcaggaacc tggttttctg tggaaggtat ggacagaaag tgaaaagaac cacgaagccg
    26401 gtgggatcta ccttttcact gatgaaaaaa gcgcccttgc ctatctggaa aaacatactg
    26461 cccgactgaa aaatctcggc gttgaggaag ttgtcgccaa agttttcgat gtcaatgagc
    26521 cacttagtca aatcaatcag gcaaaactcg cctgacagaa tttaatcaag ggcggttagc
    26581 gcccttttca tccctgtctg aaatttctca aattctaaaa atctcaacca aacttatctg
    26641 ataacactaa attcgaaaga atgcgtacag gtaagtaaca atgaaaaaaa ttgctattgt
    26701 gggtgccggg cctacgggga tctacacctt attctcgctt ctacagcaac aaactccact
    26761 ttctatttct atcttcgagc aggctgacga ggccggtgtc gggatgccat acagtgatga
    26821 ggaaaactca aaaatgatgc tggcaaatat tgccagtatt gaaataccgc cgattaattg
    26881 tacgtatctc gaatggctac aaaagcaaga agccagccat ctccagcgtt atggcgttaa
    26941 aaaagaaacc ttgcacgatc gtcagttttt accgcgaatt ctgctgggcg aatatttccg
    27001 cgatcaattt ttacgattag tagaccaggc acgacagcaa aaatttgcag tggctgttta
    27061 tgaatcatgc caggttaccg atctgcaaat tacaaatgct ggcgtcatgc tcgctacaaa
    27121 tcaggattta cccagcgaga cgtttgattt agcggtgatt gccacgggtc acgtctggcc
    27181 tgatgaagaa gaagcaacca gaacgtattt tcccagcccg tggtcaggcc tgatggaagc
    27241 aaaggtcgat gcgtgtaacg tgggtattat gggaacatcc ttgagcggac tggatgcggc
    27301 aatggcagtg gctattcagc atggttcgtt cattgaagat gataaacaac acgtcgtttt
    27361 tcaccgcgat aacgcaagtg aaaagctaaa tatcacgttg atgtcgcgca cgggtatttt
    27421 acccgaagcc gatttctatt gccctattcc ctacgagccc ttacacatcg tcaccgatca
    27481 ggcattaaat gctgagattc aaaaaggcga atatggcctt ttggatcggg tatttagatt
    27541 gatagtagag gaaatcaagt ttgctgatcc agactggagc caacgcatag ccttagagag
    27601 cctgaatgtc gattcctttg ctcaagcctg gtttgccgag cgcaaacaac gcgacccatt
    27661 tgactgggca gaaaaaaatc tccaggaagt cgaacgcaat aaacgagaaa aacatactgt
    27721 tccctggcgt tatgtcattc tgcgcctgca tgaagccgta caggaaattg ttccacatct
    27781 gaatgaacac gaccataaac ggttcagtaa aggccttgcc cgggttttca tcgataatta
    27841 tgcggcaatc ccttcagagt ctattcgtcg gctgctggcc ttacgtgaag cggggatcat
    27901 tcatattctc gccctcggtg aagactacaa aatggaaatt aatgagtcgc gcaccgtcct
    27961 gaaaacggaa gacaacagct actcgtttga cgtttttatt gatgcccgcg gacagcgtcc
    28021 gcttaaagtg aaagatattc ctttccctgg gctacgcgag caattacaga aaacagggga
    28081 tgaaatccct gatgttggcg aagattatac gttacagcaa cccgaagata ttcgtggacg
    28141 cgtagcgttc ggcgcgttgc cctggttgat gcacgaccag cctttcgttc agggacttac
    28201 ggcatgtgca gaaattggtg aggcgatggc tcgggcggtc gtaaagcctg catcccgtgc
    28261 tcgtcggcgt ctttcgtttg attaaaggta aagctatcag cactcaacaa actgatgctg
    28321 ttcattaaga cgatagcgga ctccggttcg aatattgttt tcaccttcaa tggcgacggc
    28381 gaagcgaact ctttcgccgt catgatatgc cagtgcggcg cttccccctg gcccgagaat
    28441 aattgagtcg accacgcccg tgctcactac cgttgaattt tcaccgctgg caatgatgtg
    28501 tacattgtcg ccgctgttgg caattctggc gtttgcgcca aagctggcga tttgcaccag
    28561 gtcaccatta ctggcgacat gacaccgttc acccaatgta caaacgcgta ctcgcattcc
    28621 ggtattcgca atccgactac tatctccagc gctgcttatc cgcgaagaat taccggttac
    28681 agcaacccgt gcgcgttcac cggagctgcc aatgtgactg ttaaagccga cacttccaat
    28741 ccgcacgcta taacccatac tggcaatttg cgcggcatac ccagcggtta cgattcgggc
    28801 gttatcctca caacagccga tgcgagcatg atcgtcgctg cgggcaaact gactgcaaaa
    28861 tggtatctct ccggggtctg tggccagttt ttgcatagag gaaacttcct gatgcgcgaa
    28921 gttttcatcc gccagccatt ttttccacgc atactcaacc agactctccg cccagtccgt
    28981 atatccttcc tcaatcagtg catgatgaat atcggcataa ctaccgccgc gtgggaaatg
    29041 gcgcaggaac cagcgataca tcaccgctcc gatacgccat tctcgcaggt ctgctcgtgt
    29101 aatgatcatt cgttagtgcc ggtgatagcc atcgaccatg cttttaaagg tttcgtgtgg
    29161 cgtacgcccc gtggtgcaaa gataaagatg tccgaagata aaaaagaggc ttataaatgc
    29221 cagagcaaaa tgcgcctgca ataaccagta tcttacgcca ggaaacacat ctcccacggc
    29281 ttgcggatag agacacaaca gccccgtcaa tagtaacaac ggcagcaatc cacacatgac
    29341 accaacatag gcgacctgct gtaagggatt aaatttagac tgtgttgttg ccgggaaagg
    29401 atgttcttcc ccctgcataa tgccaaacaa ataaaatcgt gtttgttttg ccgctcgttc
    29461 cagccacccc tgacgacgaa tgcgatagtg gtgaccatta tccccaacgg cattgatcag
    29521 cacaaagccg agccagcatg ccagtaacaa aaatccgcaa acttcatgca ccgcaaccag
    29581 acttttaacc acagttgcgc ccaccatcgc aaagtgattt atcagcccac ttgccagcaa
    29641 caatacaaag agtaacgcat tcgaccagtg ccataatctg accgctttgc tgtaaagata
    29701 aaccttctca ccatgatcgg tggctgattt cttcacgcca cgagcacgaa gcaaggcgtg
    29761 cagccccaac accaaccaca tcccaaccag caatactccg gcaatgatca accacaccgg
    29821 ccaaaattcg ggagtgaact gtggcacata gttcgctaac tggctctgaa actgttcagc
    29881 atgttgcgac gggttcatac attttccttt ttaatcaaat gttgaccgaa ccggcgatac
    29941 agatgcggtt tcccggcacc aagtagctga tattgatagt atttattttc ctgcaaccac
    30001 gcctgaattt ctgggctatc ttcgcgacca aagatcaatg cgtgttccgg acaggcgctg
    30061 acgcaaatgg gcggaaagcc ttttgccagt cgggactcag cgcagaaatc acatttatct
    30121 gccactttag tgacagggtt aaggtagcga acctggtacg gacaagcgcc aatgcaataa
    30181 ctgcagccga tgcactgcga tttttctacc cgcacgatcc cctgctcatc acgccacgac
    30241 gcccctgtcg ggcatacgtc gatgcacgga gcatcttcgc agtgctgaca tgactggcga
    30301 aaaaagtgat actgcgtttc gttgtcgtta tcggtgactg gaatgtgtgc tatcgataaa
    30361 cggcttccct gagcggggac gtggttagtt ttacggcatg cgcgggcgca aatattacag
    30421 ccattgcacc gtgactcgtc atgaatcatg gcataccgaa cttccttttc ttgcctcgtg
    30481 ttcgctaaca gagaagacgc agaaccagta aaaaagatta ctgttcccat ccccagaaca
    30541 aatttgcgtc gagtgaagga catctgatta tcccctaatg cagtggctgc aactgtccac
    30601 cctatccatg acatctccct gaaatttcag gggttctgaa actgctctac ctcgaaccat
    30661 tcgcataagc cataagcctt gccataggtc ataaatcgct cgctaaacaa gtacaacacc
    30721 gccgtcgatg actccacctg ccgtatatcg gcatattcag gtgcaacatc catcgcggcg
    30781 atcgcggctt caatctgcgc ttcctggaag taatatggcg cttgcatcag catggcgact
    30841 ttgtaaggac gaggatacgt ctggcactca aaacgaacgg cgtgtgcaat ggcgcgacaa
    30901 atgtcctgct ccacaacttg caaagacatc gccgcgtagt tttcactcat ggcctgcgtt
    30961 gagtaatagt aatcatcctg acttcccgtc atacaagcga tatccgcgta gtctccgttc
    31021 tgcttaatgc tttccagcaa aactgacagt tcctcttctg caaccgaata gggtggcgag
    31081 agaaacacgg tgcgcgccac cagctgccca gccgcagaat gctggcgaat gaactcagcc
    31141 aggatttcag caggttccgg ttcggttacc gtatcaacgg ggtgacgcat cccctgcaac
    31201 cattgccgct tcgcctcgtc aacttcgctg actttcgcta acggcaattc atcctgatgg
    31261 ttcatcttac ccattcctta cgcaggcagt agattgtgtg ctgccagatc ggcggcaata
    31321 tcttccagcc ccagccgctg caatgtttcg cgggttggac aaccaagctg tgggtcccag
    31381 cccatttctt tgtagaacat cgtgagcgaa gcatgcatat cgtcacgatc cattttgtca
    31441 gtaccttcag taaagaccgg gatctgcgga tccttgtcga aaacccagga acagataaga
    31501 tcgtgttcat tacgcatatc tttggtttgc atcagtttta ccgtgtaggc acgatgcaac
    31561 gtaaagatac gctctgcagc taaatctaat ttttcctgag tcatctcttc gccggtgatc
    31621 gctttgaaga atttggcttc aagcgccaga tcgccccggt aattacggct tttcagcggc
    31681 gaaacggtca ttggccagac ccagttgcac agggtgacgg cgttatgcaa acagacccgc
    31741 aacagcgacc actttgcata tttaattttt gcgtcattga ttggcgtgta gtttttggtt
    31801 tcatcgtaag catcttcaga accaaaaagt tctttcgcca cttcacgttg cagtttcaat
    31861 ggcaagccg
//
LOCUS       DAZPAC010000026        31243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZPAC010000026 DAZPAC010000000
VERSION     DAZPAC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.026"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..152
                     /locus_tag="ACHUPM_000990"
     CDS             <1..152
                     /locus_tag="ACHUPM_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868844.1"
                     /translation="LREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKR
                     LGMKD"
     gene            complement(149..1114)
                     /gene="gutQ"
                     /locus_tag="ACHUPM_000991"
     CDS             complement(149..1114)
                     /gene="gutQ"
                     /locus_tag="ACHUPM_000991"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR0868845.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(1107..1880)
                     /gene="srlR"
                     /locus_tag="ACHUPM_000992"
                     /gene_synonym="gutR"
     CDS             complement(1107..1880)
                     /gene="srlR"
                     /locus_tag="ACHUPM_000992"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR0868846.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(1947..2306)
                     /gene="gutM"
                     /locus_tag="ACHUPM_000993"
     CDS             complement(1947..2306)
                     /gene="gutM"
                     /locus_tag="ACHUPM_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR0868847.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            complement(2412..3180)
                     /gene="srlD"
                     /locus_tag="ACHUPM_000994"
                     /pseudo
     CDS             complement(2412..3180)
                     /gene="srlD"
                     /locus_tag="ACHUPM_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            complement(3184..3555)
                     /gene="srlB"
                     /locus_tag="ACHUPM_000995"
                     /gene_synonym="gutB"
     CDS             complement(3184..3555)
                     /gene="srlB"
                     /locus_tag="ACHUPM_000995"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR0868848.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(3566..4525)
                     /gene="srlE"
                     /locus_tag="ACHUPM_000996"
                     /gene_synonym="gutE"
     CDS             complement(3566..4525)
                     /gene="srlE"
                     /locus_tag="ACHUPM_000996"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR0868849.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(4522..5085)
                     /gene="srlA"
                     /locus_tag="ACHUPM_000997"
                     /gene_synonym="gutA"
     CDS             complement(4522..5085)
                     /gene="srlA"
                     /locus_tag="ACHUPM_000997"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFR0868850.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFLHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            5341..6426
                     /gene="mltB"
                     /locus_tag="ACHUPM_000998"
     CDS             5341..6426
                     /gene="mltB"
                     /locus_tag="ACHUPM_000998"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR0868851.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            6571..7068
                     /gene="pncC"
                     /locus_tag="ACHUPM_000999"
     CDS             6571..7068
                     /gene="pncC"
                     /locus_tag="ACHUPM_000999"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR0868852.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            7148..8209
                     /gene="recA"
                     /locus_tag="ACHUPM_001000"
     CDS             7148..8209
                     /gene="recA"
                     /locus_tag="ACHUPM_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR0868853.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            8437..8937
                     /gene="recX"
                     /locus_tag="ACHUPM_001001"
     CDS             8437..8937
                     /gene="recX"
                     /locus_tag="ACHUPM_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR0868854.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            9065..11695
                     /gene="alaS"
                     /locus_tag="ACHUPM_001002"
     CDS             9065..11695
                     /gene="alaS"
                     /locus_tag="ACHUPM_001002"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR0868855.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            11930..12115
                     /gene="csrA"
                     /locus_tag="ACHUPM_001003"
     CDS             11930..12115
                     /gene="csrA"
                     /locus_tag="ACHUPM_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR0868856.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            12431..12523
                     /locus_tag="ACHUPM_001004"
     tRNA            12431..12523
                     /locus_tag="ACHUPM_001004"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12465..12467,aa:Ser,seq:gct)
     gene            12527..12603
                     /locus_tag="ACHUPM_001005"
     tRNA            12527..12603
                     /locus_tag="ACHUPM_001005"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12561..12563,aa:Arg,seq:acg)
     gene            12667..12743
                     /locus_tag="ACHUPM_001006"
     tRNA            12667..12743
                     /locus_tag="ACHUPM_001006"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12701..12703,aa:Arg,seq:acg)
     gene            12942..13018
                     /locus_tag="ACHUPM_001007"
     tRNA            12942..13018
                     /locus_tag="ACHUPM_001007"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12976..12978,aa:Arg,seq:acg)
     gene            13299..13865
                     /gene="yqaB"
                     /locus_tag="ACHUPM_001008"
     CDS             13299..13865
                     /gene="yqaB"
                     /locus_tag="ACHUPM_001008"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR0868857.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            13862..14290
                     /locus_tag="ACHUPM_001009"
     CDS             13862..14290
                     /locus_tag="ACHUPM_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR0868858.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            14363..15919
                     /gene="gshA"
                     /locus_tag="ACHUPM_001010"
     CDS             14363..15919
                     /gene="gshA"
                     /locus_tag="ACHUPM_001010"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR0868859.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            16069..16584
                     /gene="luxS"
                     /locus_tag="ACHUPM_001011"
     CDS             16069..16584
                     /gene="luxS"
                     /locus_tag="ACHUPM_001011"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR0868860.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(16648..18186)
                     /gene="emrB"
                     /locus_tag="ACHUPM_001012"
     CDS             complement(16648..18186)
                     /gene="emrB"
                     /locus_tag="ACHUPM_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR0868861.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(18203..19375)
                     /gene="emrA"
                     /locus_tag="ACHUPM_001013"
     CDS             complement(18203..19375)
                     /gene="emrA"
                     /locus_tag="ACHUPM_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFR0868862.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(19502..20032)
                     /gene="emrR"
                     /locus_tag="ACHUPM_001014"
     CDS             complement(19502..20032)
                     /gene="emrR"
                     /locus_tag="ACHUPM_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR0868863.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(20123..20458)
                     /gene="ygaH"
                     /locus_tag="ACHUPM_001015"
     CDS             complement(20123..20458)
                     /gene="ygaH"
                     /locus_tag="ACHUPM_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR0868864.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(20448..21185)
                     /gene="ygaZ"
                     /locus_tag="ACHUPM_001016"
     CDS             complement(20448..21185)
                     /gene="ygaZ"
                     /locus_tag="ACHUPM_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR0868865.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(21309..22493)
                     /locus_tag="ACHUPM_001017"
     CDS             complement(21309..22493)
                     /locus_tag="ACHUPM_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0868866.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(22784..23776)
                     /gene="proX"
                     /locus_tag="ACHUPM_001018"
     CDS             complement(22784..23776)
                     /gene="proX"
                     /locus_tag="ACHUPM_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR0868867.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(23834..24898)
                     /gene="proW"
                     /locus_tag="ACHUPM_001019"
     CDS             complement(23834..24898)
                     /gene="proW"
                     /locus_tag="ACHUPM_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR0868868.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(24891..26093)
                     /gene="proV"
                     /locus_tag="ACHUPM_001020"
     CDS             complement(24891..26093)
                     /gene="proV"
                     /locus_tag="ACHUPM_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR0868869.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(26449..27408)
                     /gene="nrdF"
                     /locus_tag="ACHUPM_001021"
     CDS             complement(26449..27408)
                     /gene="nrdF"
                     /locus_tag="ACHUPM_001021"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0868870.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(27418..29562)
                     /gene="nrdE"
                     /locus_tag="ACHUPM_001022"
     CDS             complement(27418..29562)
                     /gene="nrdE"
                     /locus_tag="ACHUPM_001022"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0868871.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTNEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(29535..29945)
                     /gene="nrdI"
                     /locus_tag="ACHUPM_001023"
                     /pseudo
     CDS             complement(29535..29945)
                     /gene="nrdI"
                     /locus_tag="ACHUPM_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(29942..30187)
                     /gene="nrdH"
                     /locus_tag="ACHUPM_001024"
     CDS             complement(29942..30187)
                     /gene="nrdH"
                     /locus_tag="ACHUPM_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR0868872.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(30435..30764)
                     /locus_tag="ACHUPM_001025"
     CDS             complement(30435..30764)
                     /locus_tag="ACHUPM_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFR0868873.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            30916..>31243
                     /locus_tag="ACHUPM_001026"
     CDS             30916..>31243
                     /locus_tag="ACHUPM_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR0868874.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGL"
BASE COUNT         7713 a         7906 c         8268 g         7356 t
ORIGIN      
        1 acctgcgtga agcgacagaa gcgttccagc gtgaaactat tcgccaggca ctggcacaaa
       61 atcatcataa ctgggctgcc tgcgcgcgga tgctggaaac cgacgtcgcc aacctgcatc
      121 ggctggcgaa acgtctggga atgaaggatt aaataatccc ggcctgatag aaatcctgca
      181 ggttaattgc gccggtgagt ttgccgtttt catccaccac cggtgcggca gtgattttgc
      241 gcttcatcag gatctctttg gcgtcgatgg cgcgactttg cgcctgtaac gtggtgccgc
      301 ctgtcgtcat cgcttcatta actggcgtgg tgagtgcgcc gccgcctacc agccagcgac
      361 gtaaatcgcc gtcggtaaag acgccttgta cctgttgttg agcgtcacat accgccacca
      421 gccccagacc ggtgcggctg agttccagca tcgcatccat cacgctggcg gttaacgcca
      481 cctgtgggat ggcatcgtca cggcgcatca gatgatgcac tttattcagc aagcgagcgc
      541 ccagtgcccc ggctgggtgg gagcgggcaa aatcttcttc attaaatccg cgcgcctgca
      601 tgaccgccat cgccagcgcg tcacccatca tcagggtatt gacggtgctg gaggtcggcg
      661 caaggtgcat cgggcaggct tcgcgttcta cggagatatc cagcaccgct tttgccgcca
      721 ggcccagcgg tgacgtcggt ttgccggtca tcgccagcag cgcgatagat ttatcttcca
      781 gacgcggaat aatcagatcc agttccttcg cgccaccgga gtaagagata aacagcatca
      841 catcgcggct ttcaatcatc cccagatcgc cgtgcagtgc ttctgccgga tggacaaaaa
      901 aagccgatgt gccggtactg gcaagtgttg cggcgatttt cttaccaatg tggcccgatt
      961 tgccaattcc cgaaaccacc actttgcctt cgcagtgcag gatgatattg gcggcgcgaa
     1021 caaaatcatc gcccagacgt tccggtaaac ggcttgcttc ctgcaactcc agcattaacg
     1081 tctgacgtcc cgcgttcagt agtgcttcac tcattgctct ctccggttat gatcacatcg
     1141 atccctttct cttccagcgc ctgacgaaac gccggatcga tacctgcgtc ggtaatcagc
     1201 ttatcgacgc tttcaagact gcaaactacg ttggggcttt tacggccaaa ctttgatgag
     1261 tccgccatca aaatcacttc gcgcgcggca ttgcacattg ccttactgac ggtataaacc
     1321 tcgttaaagg tggttacgcc cgcattgaga tcgatgccgt cggtgcccat aaacaattta
     1381 tcgaaactga aatgttcgaa ggcattctct gccagctgtc cgtgaaatga ggccgatttt
     1441 ttacgaaacg ttccgcctgg catcaggata gtttgttcgt tatccagttc agatagcgca
     1501 ttgacgatat gcagactgtt ggtcatcacc gtgatgttat taaagcgcga gagcagggga
     1561 accatctgca aaacggtact gccagcatca agaatgatcg aatcgccatc atgaataaaa
     1621 ctaacggcag cttctgctat cagctctttc ttgtgggtgt tgatgagtgt tttatgatcg
     1681 ataggcggat cggattcctc tttattcaac accactccgc cataagtacg aatgacggtt
     1741 cctgcatgtt ccagaatgac cagatctttg cgaatggttg tgcctgtggt gtcaaagtat
     1801 tgcgccaatt cttcaaccga gcatttaccc tgcttttgca gatactccag aatggcggcc
     1861 tgacgctgac gaggtttcat gggcgtgatt acctgattta ggtttcaaaa tgataacttc
     1921 gcaagcaagt aacattcaca acgaaattat ccacgtttca gtttaagcgc caatgataga
     1981 gcattctgtg atagtggatc atggggaaag atcacatcgg gctgtaaatc acccgcatgc
     2041 ataccggtaa ttgcgggaat tttttgcggt cgtgcgaaga cggtcagtcc tttcataaac
     2101 aaggtgtcga cgatgcgctg ctgatcgtcc agggcgatgg cgaccacgac ccgtggttta
     2161 aagcgcccgc tggaacggcc cacgccaacc cgcccttgct ggcatagtgt gtcgaaggca
     2221 cggttaaaac gagaaatttg ccagccgcct aaggccagtt gcgcacacca ggcgataacg
     2281 gcgacggtga tgagtgcgga taccataact tttccttaat gtagtggcgg aagtcctgta
     2341 tgcctgatga gcgtagcgca tcaggcattc tattagtctc acggagatcc ttaacggatc
     2401 tccgctgttg attacaacat cacctgaccg ccggtgacat tgatcgactg tccggtgcag
     2461 tacgacgcct taggactggc atagagcagc agcatattca gcacatcctg ataatcgcag
     2521 ccgcgtttga gcggtacttt gtcgatgtaa tactgctcga cttgatccgg tttgataccc
     2581 agcttggtcg cgtattgggg taacagtgac tggaacatag gcgatttcag caggttaccg
     2641 agcatcagtg aatgcaccgt aatgccgtac tccgccagat ccagcgccag tgattgagtc
     2701 agcccgacgc caccaaattt cgctgcgctg tagccagagt tgtgtttgct gcccactttg
     2761 ccggatttcg agttgatctg aataatgcga ccctgaatcc cgtcgcggat catcaaacga
     2821 gaaaattcac gggcacacag gaaataaccc accagattca cggtcaaaat cgccgagctg
     2881 gaagtcgctg ataaaggctg cttttgctat tccggcgctg tagaccagca aatccacgcg
     2941 cccaaagatt tcatctaccc cacgagagag cgccaggacg ctttgctcgc tggtggcgtc
     3001 agcaccaaaa ctatacgctg ttccttcacc atattcggcg ttaatttctt gtgccacatt
     3061 tgcggctttg tcgctctgaa tatcgacaac cgcgacgcga tacccctcgg cagccagacc
     3121 gtggcacagg aacgcgccta aggtttgccc accaccgatg acaacggcaa cctgattcat
     3181 tttttactcc ttaacagatt caaacttcaa aaccgatccc ggcgcgatat cgtcgggtac
     3241 agggcctgcc acatggacag tgcccggaaa ttccgcttcg tttaaaccat cgaagcgcag
     3301 ggtgacatga cccagttcgc gaaggttgtc ttccgccacg ctgccaacag cggtcaccgg
     3361 atagcgatgc tgcccgagcg taaattgcaa accgggatgg agtgcacctt tcaactcgcc
     3421 gtggcaatga atgaagcaat actcttcgag gtccgctggc gcgccttcac gaaaggtgat
     3481 gagcatctgg tcgctgaggg cgtcagtggc actcgcgccg atacgggtga tggtggtctg
     3541 ataaataacg gtcatgtttc agcctctatt gatagataaa accggagaca aaccaggcga
     3601 tcagtacagt cggtgcgccg gttaaaaagt ggctcaccag tacagaaggg acaccgacgc
     3661 gaaccgtgtc ctgacgggct tccgccagcg acaaaccgac cgggatgaag tcgcaggccg
     3721 cctgcgcgtt gatggcaaac agtgccggta aagccagatg cggcggaata ttgccgagac
     3781 caatctgcac gccaatcaat acgccgataa cctgtgcgat aactgcgcct gggccgagga
     3841 aaggtgaaag cagcgggaag gagcagatga gcgccagcat gaccagacct agtggatggc
     3901 tcgccagcgg agcaagaccg tgggcaatcc agtcaccaag gccagaagcc ataatgatgc
     3961 caatgagcgc cgagacgaac gccataaacg gcagaatggt ttttaataca gtgtcgatgg
     4021 tgtcacgacc agattgaaac aacacggcaa cggcagaacc catacccatt cccaccttcg
     4081 ccagtaaacc atcgctttgt tcggtgattt tcttgctggt gtcatagtca cggcccacgg
     4141 aagagggttg tggtgtcgca tcacccacta cagtgatgtt ttcttctttt acgccagaga
     4201 cataaatatc ttccacaatg tactgcgcca gcggactgga cttgcccgtc gagtggatat
     4261 taatggtggg aatacgtcgt ttcggataga tgccgcagcg taatgtgccg ccacagtcga
     4321 ttaccgcgac accaatttcc gcctccgcgg gttcaccttc tttaaatccg tcaatagcct
     4381 gccagccagt aagttgtgcc agtttgtcaa caatcgcagg ccgggtaccg gcggtgatat
     4441 agacgatttt tttgcccggc gtggcttcca gctcaagcgg gccgccccag ccacccgttc
     4501 ctttttcgat ccgaatacgc gtcatgatgt tgctcctgcc aggtgaactt tttgttcaag
     4561 ttgaatgccc atctttttct caaaaatggc ggtggtcaga tcggtcaccc agccgcgaaa
     4621 gaagttggtg accagaccaa ccagcagata gctcaccgcc agtgggccaa gtggcaggtt
     4681 cagcgttgtc agaccgctgg caatgccaag ataaacaaac agttcgccag ggttgatatg
     4741 gagaaagagg ccattcattg agtggcagct ataagaggct gccgcgtagt agctgggttt
     4801 gtacttttcc ggcataaagc gacccaggct taaggtcatc ggattgcaaa agacaaacgt
     4861 gccaatgcac ggtagcagca ggtaacggga aacagggtta ccggcgcaac gttgagcaaa
     4921 acgttcaata cgatgctgac cgataaaatt aatcagtgcg ttcatgataa ccagcaggct
     4981 aatcaacagc ggaagaatgc cggtcaccat cccggtaaac acctctccgc ccttttggaa
     5041 cagcccgata aaccactctg caccatgagt aatggtttct atcattgttc tctccttcag
     5101 gatttattgt tttattacca aacggcaacc taatctaatc agattgaaag atttgaaagt
     5161 gttattttga tcgcaaaatg aaagataaat attttaattt gaaagtttga ataaaaggat
     5221 agcgagggga atgagttgag ttatgtaaag tccgtatcgg gcagtgacta ccgcttcctt
     5281 gtgcggggcg tgatgcttta ccatacttgc ccctggttga atctgttaaa tggacccctc
     5341 atgttcaagc gtcgttatgt aacattgctt cccctttttg tgttgcttgc cgcctgtagc
     5401 agcaagccaa aacctactga gactgaaacg accaccggaa cgccgtctgg cggcttcctg
     5461 cttgagcctc agcacaatgt gatgcagatg ggcggcgatt tcgctaataa cccgaatgcc
     5521 cagcagttca tcgacagaat ggtgaacaaa cacggtttcg atcgtcagca gttgcaggaa
     5581 attctctccc aggcgaagcg tctggattcg gtactgcggc tgatggataa ccaggcacca
     5641 accacatcgg tgaaaccgcc atcaggtccg aacggcgcat ggctgcgtta tcgcaaaaaa
     5701 tttattacgc cggacaacgt gcagaacggt gtggttttct ggaatcagta tgaagatgcg
     5761 ttgaatcgcg cgtggcaggt gtatggcgta ccgccggaaa ttatcgtcgg gattatcggc
     5821 gttgaaaccc gctgggggcg cgtgatgggg aaaactcgca tcctcgatgc gctggcaacg
     5881 ctgtcattta actacccacg ccgcgcggag tatttctctg gcgagctgga aaccttcctg
     5941 ttgatggcgc gcgacgagca ggacgatccg ctcaatctga aaggttcctt tgccggggcg
     6001 atgggctacg gacagtttat gccgtcgtct tacaaacaat atgcggtaga tttcagcggc
     6061 gacgggcata tcaacctgtg ggatccggtt gatgcgatcg gtagtgtggc gaactatttc
     6121 aaagcgcacg gctgggtgaa aggcgatcag gtcgcggtaa tggcaaacgg tcaggctcca
     6181 ggcttgccaa atggcttcaa aaccaggtac agcatttcgc agcttgccac ggcaggttta
     6241 acgccacagc agccgctggg caaccatcaa caagccagcc tgctgcgtct ggatgtcggt
     6301 accggttacc agtactggta cggtctgccg aacttctaca ccattacccg ttataaccac
     6361 agcacccatt acgcaatggc ggtctggcag ttaggacaag ccgtggcgct ggcgcgcgtg
     6421 cagtaactat ttgcaagagg ggggcgtgag cttccctctg gctaatcagc ttttttctga
     6481 atcctcctcg taaaattgca acgccaacac catcttcctg acgaaagtgc tatcttgtcc
     6541 ggcataaatt tttgactgac tgaggttgtg atgactgaca gtgaactgat gcagttaagc
     6601 gaacaggttg ggcaggcgct gaaagcccgt ggcgcaaccg taacaactgc cgagtcttgt
     6661 accggtggtt gggtagcgaa agtgattacc gatattgccg gtagctccgc ctggtttgaa
     6721 cgcggatttg tcacctacag taacgaagcc aaagcgcaga tgatcggcgt acgcgaagag
     6781 acgctggcgc agcatggcgc ggtgagtgaa cccgtcgtgg tggaaatggc gataggtgca
     6841 ctgaaagcgg ctcgtgctga ttatgccgtg tctattagtg gtatcgccgg gccggatggc
     6901 ggcagtgaag agaagcctgt cggcaccgtc tggtttgctt ttgccactgc ccgcggtgaa
     6961 ggcattaccc ggcgggaatg cttcagcggc gaccgtgatg cggtgcgtcg tcaggctact
     7021 gcgtatgcat tgcagacctt gtggcaacaa tttctacaaa acacttgata ctgtatgagt
     7081 atacagtata attgcttcaa cagaacatat tgactatccg gtattacccg gcatgacagg
     7141 agtaaaaatg gctatcgacg aaaacaaaca gaaagcgttg gcggcagcac tgggccagat
     7201 tgagaaacaa tttggtaaag gctccatcat gcgcctgggt gaagaccgtt ccatggatgt
     7261 ggaaaccatc tctaccggtt cgctttcact ggatatcgcg cttggggcag gtggtctgcc
     7321 gatgggccgt atcgtcgaaa tctacggacc ggaatcttcc ggtaaaacca cgctgacgct
     7381 gcaggtgatc gccgcagcgc agcgtgaagg taaaacctgt gcgtttatcg atgctgaaca
     7441 cgcgctggac ccaatctacg cacgtaaact gggcgtcgat atcgataacc tgctgtgctc
     7501 ccagccggac accggcgagc aggcactgga aatctgtgac gccctggcgc gttctggcgc
     7561 agtagacgtt atcgtcgttg actccgtggc ggcactgacg ccgaaagcgg aaatcgaagg
     7621 cgaaatcggc gactctcaca tgggccttgc ggcacgtatg atgagccagg cgatgcgtaa
     7681 gctggcgggt aacctgaagc agtccaacac gctgctgatc ttcatcaacc agatccgtat
     7741 gaaaattggt gtgatgttcg gtaacccgga aaccactacc ggtggtaacg cgctgaaatt
     7801 ctacgcctct gttcgtctcg acatccgtcg tatcggcgcg gtgaaagagg gcgaaaacgt
     7861 ggtgggtagc gaaacccgcg tgaaagtggt gaagaacaaa atcgctgcac cgtttaaaca
     7921 ggctgaattt cagatcctct acggcgaagg tatcaacttc tacggcgaac tggttgacct
     7981 gggcgtaaaa gagaagctga tcgagaaagc aggcgcgtgg tacagctaca aaggtgagaa
     8041 gatcggtcag ggtaaagcga atgcaactgc ctggctgaaa gataacccgg aaaccgcgaa
     8101 agagatcgag aagaaagtac gtgagttgct gctgagcaac ccgaactcaa cgccggattt
     8161 ctctgtagat gacagcgaag gcgtagcaga aactaacgaa gatttttaat cgttcgttat
     8221 tgatgataat agataatgtg tgcacggacg gtcccctcgc ccctttgggg agagggttag
     8281 ggtgagggga acaggccaac actggcgtga aaaattactc tcacctcgat cctctaacga
     8341 attggctgca cactcattga ctattaaatc tgtttatcag caaaacctgg gtcgcatctg
     8401 cggccctttt gcttttttaa gttgtaagga tatgccatga cagaatcaac atcccgtcgc
     8461 ccggcatatg ctcgcctgtt ggatcgtgcg gtacgcattc tggcggtgcg cgatcacagt
     8521 gagcaagaac tgcgacgtaa actcgcggca ccgattatgg gcaaaaatgg cccagaagag
     8581 attgatgcta cggcagaaga ttacgagcgc gttattgcct ggtgccatga acatggctat
     8641 ctcgatgaca gccgatttgt tgcgcgcttt atcgccagcc gtagccgcaa aggttatgga
     8701 cctgcacgta ttcgtcagga actgaatcag aaaggtattt cccgcgaagc gacagaaaaa
     8761 gcgatgcgtg aatgtgacat cgactggtgc gcactggtgc gcgatcaggc gacgcgaaaa
     8821 tatggtgaac ctttgccaac tgtcttttca gaaaaagtta agatccagcg ttttctgctc
     8881 tatcgtggct atctgatgga agatatccag gatatttggc taaattttgc cgactgaacg
     8941 catacggtat tttacttccc actcaagaaa acttatctta ttcccacttt tcagttacca
     9001 gcccggcggt taagacacgc tggagctggt ggcgatattt cgttagcttg atttcaggat
     9061 aattatgagc aagagcaccg ctgagatccg tcaggcgttt ctcgactttt tccatagtaa
     9121 gggacatcag gtagttgcca gcagctccct ggtaccccat aacgacccaa ctttgttgtt
     9181 taccaacgcc gggatgaacc agttcaagga tgtgttcctt ggactcgaca agcgtaatta
     9241 ttcccgcgct accacttccc aacgctgcgt gcgtgcgggt ggtaaacaca acgacctgga
     9301 aaatgtcggt tacaccgcgc gtcaccatac cttcttcgaa atgctgggca acttcagctt
     9361 cggcgactat ttcaaacacg atgccattca gtttgcatgg gaactgctga ccagcgaaaa
     9421 atggtttgcc ctgccgaaag agcgtctgtg ggttaccgtc tatgaaagcg acgacgaagc
     9481 ctacgaaatc tgggaaaaag aagtagggat cccgcgcgaa cgtattattc gcatcggcga
     9541 taacaaaggt gcgccatacg catctgacaa cttctggcag atgggtgaca ctggtccgtg
     9601 cggcccgtgc accgaaatct tctacgatca cggcgaccac atttggggtg gccctccggg
     9661 aagtccggaa gaagacggcg accgctacat tgagatctgg aacatcgtct tcatgcagtt
     9721 caaccgccag gcagatggca cgatggaacc gctgccgaag ccgtctgtag ataccggtat
     9781 gggtctggag cgtattgctg cggtgctgca acacgttaac tctaactatg acatcgacct
     9841 gttccgcacg ttgatccagg cggtagcgaa agtcactggc gcgaccgatc tgagcaataa
     9901 atcgctgcgc gtaatcgctg accacattcg ttcttgtgcg ttcctgatcg cggatggcgt
     9961 aatgccgtcc aatgaaaacc gtggttatgt actgcgtcgt atcattcgtc gcgcagtgcg
    10021 tcacggcaat atgctcggcg cgaaagaaac cttcttctac aaactggttg gtccgctgat
    10081 cgacgttatg ggctctgcgg gtgaagacct gaaacgccag caggcgcagg ttgagcaggt
    10141 gctgaagact gaagaagagc agtttgcccg tactctggag cgcggtctgg cgttgctgga
    10201 tgaagagctg gcaaaacttt ctggtgatac gctggatggt gaaacggctt tccgtctgta
    10261 cgacacctat ggcttcccgg ttgacctgac ggctgatgtt tgtcgtgagc gcaacatcaa
    10321 agttgacgaa gctggttttg aagcagcaat ggaagagcag cgtcgtcgcg cgcgcgaagc
    10381 cagcggcttt ggtgccgatt acaacgcaat gatccgtgtt gatagtgcat ctgaatttaa
    10441 aggctatgac catctggaac tgaacggcaa agtgaccgcg ctgtttgttg atggtaaagc
    10501 ggttgatgcc atcaatgcag gccaggaagc tgtggtcgtg ctggatcaaa cgccattcta
    10561 tgcggaatcc ggcggtcagg ttggtgataa aggcgaactg aaaggcgcta acttctcctt
    10621 cgcggtggaa gatacgcaga aatacggcca ggcgattggt cacatcggta aacttgctgc
    10681 gggttctctg aaagtgggcg acgctgtgca ggctgatgtt gatgaggctc gtcgcgcccg
    10741 tattcgtctg aatcactccg caacgcacct gatgcacgct gcgctgcgcc aggttctggg
    10801 gactcatgta tcgcagaaag gttcactggt taacgacaag gtgctgcgct tcgacttctc
    10861 acacaacgaa gcgatgaaac cggaagagat tcgtgcggtc gaagacctgg tgaacgcaca
    10921 gattcgtcgc aatttgccga tcgaaaccaa catcatggat ctcgaagcgg cgaaagcgaa
    10981 aggtgcgatg gcgctgttcg gcgagaagta tgatgagcgt gtacgcgtgc tgagcatggg
    11041 cgatttctcc accgagctgt gtggcggtac tcacgccagc cgcactggtg atattggtct
    11101 gttccgcatc atctctgaat cgggtactgc tgcaggcgtt cgtcgtatcg aagcggtaac
    11161 cggagaaggc gctatcacca ccgttcatgc agacagcgat cgcttaagcg aagtcgcgca
    11221 tctgctgaaa ggcgatagca ataatctggc ggataaagtg cgctcagtac tggaacgtac
    11281 gcgtcagtta gaaaaagaac tacaacagct taaagaacaa gctgccgcac aggagagcgc
    11341 aaatctttcc agtaaggcaa ttgatgttaa tggtgttaag ctgttggtta gcgagcttag
    11401 cggtgttgag ccgaaaatgt tgcgtaccat ggttgacgat ttaaaaaatc agctggggtc
    11461 gacaattatc gtgctggcaa cggtagccga aggtaaggtt tctctgattg caggcgtatc
    11521 taaggacgtc acagatcgtg tgaaagcagg ggaactgatt ggtatggtcg ctcagcaggt
    11581 gggcggcaag ggtggtggac gtcctgacat ggcgcaagcc ggtggtacgg atgctgcggc
    11641 cttacctgca gcgttagcca gtgtgaaagg ctgggtcagc gcgaaattgc aataatataa
    11701 gcgtcaggca atgccgtgga ctcgcttcac ggcattcgca ttaacgctat cgacaacgat
    11761 aaagtcaggt tgaagttgtg tatatcggct aaacttaggt ttaacagaat gtaatgccat
    11821 gactgcttac atgtaatgtg tttgtcattg cttacttttt ggcgttatat gatggataat
    11881 gccgggatac agagagaccc gactctttta atctttcaag gagcaaagaa tgctgattct
    11941 gactcgtcga gttggtgaga ccctcatgat tggggatgag gtcaccgtga cagttttagg
    12001 ggtaaagggc aaccaggtac gtattggcgt aaatgccccg aaggaagttt ctgttcaccg
    12061 tgaagagatc taccagcgta tccaggctga aaaatcccag cagtccagtt actaatcttt
    12121 ccgcgtctca tctttatcgg tgagacgcac cctcaaaatt tctccttcac tctatagtct
    12181 tttcgcttta ctcccgttca ttcaacttaa gtctccattt ttttgcatta ctgctatctg
    12241 ccagacctcc gtttttctgt agataaaaca ctctttttga cgtttttaca gactaattga
    12301 gcgtgaagtg tgcaaacgat aaaagtgtgg gaaaaattgt ttgacttata agtctcagaa
    12361 agtaatatgt gcgccacgca gcgacgatga gcgataaaca agttcttcga agcactcgta
    12421 agaggcgtgt ggtgaggtgg ccgagaggct gaaggcgctc ccctgctaag ggagtatgcg
    12481 gtcaaaagct gcatccgggg ttcgaatccc cgcctcaccg ccatttgcat ccgtagctca
    12541 gctggataga gtactcggct acgaaccgag cggtcggagg ttcgaatcct cccggatgca
    12601 ccatctctta cttgatatgg ctttagtagc ggtatcaata tcagcagtaa aataaatttt
    12661 cccgatgcat ccgtagctca gctggataga gtactcggct acgaaccgag cggtcggagg
    12721 ttcgaatcct cccggatgca ccatattctc cgaaatcttc agcaatgaag gtatcgaaga
    12781 ggtagcggaa ttaaccgcag gcaccaggga tgataacgtt gctttagcaa cggcccgaag
    12841 ggcgagccgc aaggcgagta atcctcccgg atgcaccatc tcttaattga tatggcttta
    12901 gtagcggtat caatatcagc agtagaataa gttttcccga tgcatccgta gctcagctgg
    12961 atagagtact cggctacgaa ccgagcggtc ggaggttcga atcctcccgg atgcaccata
    13021 ttctccgtaa ccttcagcaa tgaaggtatc gaagagatag cggtattaac cgcaggcacc
    13081 aggaaggata acgttgcgcc agcaacggcc cgaagggcga acgtcagtga gtcatcctcc
    13141 cggatgcacc atcttctcca aaaaatttcc ttcaaaaata tctcatttct aaataactcc
    13201 tttatccatt tcctgaattt tcttctcctt cgccatcagc gacaaaatca agcaattacg
    13261 ctaaagtagc gtcatatttt tcgacttgcg agaagaccat gtacgagcgt tatgcaggtt
    13321 taatttttga tatggatggc acaatcctgg atacggagcc tacgcaccgt aaagcgtggc
    13381 gcgaagtatt agggcactac ggtcttcagt acgatattca ggcgatgatt gcgcttaatg
    13441 gatcgcccac ctggcgtatt gctcaggcaa ttattgagct gaatcaggcc gatctcgacc
    13501 cgcatgcgtt agcgcgtgaa aaaacagaag cagtaagaag tatgctgctg gatagcgtcg
    13561 aaccgcttcc tcttgttgat gtggtgaaaa gttggcatgg tcgtcgccca atggctgtag
    13621 gaacggggag tgaaagcgcc atcgctgagg cattgctggc gcacctggga ttacgccatt
    13681 attttgacgc cgtcgtcgct gccgatcacg tcaaacacca taaacccgcg ccagacacat
    13741 ttttgttgtg cgcgcagcgt atgggcgtgc aaccgacgca gtgtgtggtc tttgaagatg
    13801 ccgatttcgg tattcaggcg gcccgtgccg caggtatgga cgctgtggac gttcgcttgc
    13861 tgtgagtgaa gcgttatcgc ttttctcgtt gtttgccagt agttttctca gcgctacact
    13921 attacccggc aactccgaag tcgtgctggt ggcaatgttg ctttccggga tcagtcatcc
    13981 ctgggtttta gttttaacag caacaatggg taatagcctt ggagggttaa ctaacgttat
    14041 ccttgggcgt ttctttccat tgcgtaaaac atcgcgctgg caagagaaag ctatcggctg
    14101 gctgaaacgc tatggtgcag tcacgctatt attaagctgg atgcccgtgg ttggcgattt
    14161 actgtgtctg ttagcgggat ggatgcgcat ctcgtgggga ccggtaatct tttttttgtg
    14221 ccttggtaaa gcgttacgct atgttgcagt tgcagcagcg accgttcagg gcatgatgtg
    14281 gtggcactaa ttgtaggcct gcacatatgg tcaccattac agttatgcta attaaaacga
    14341 ttttgacagg cgggaggtca atttgatccc ggacgtatca caggcgctgg cctggctgga
    14401 aaaacatcct caggcgttaa aggggataca gcgtgggctg gagcgcgaaa ctttgcgtgt
    14461 taatgctgat ggcacactgg caacaacagg tcatcctgaa gcattaggtt ccgcactgac
    14521 gcacaaatgg attactaccg attttgcgga agcattgctg gaattcatta caccagtgga
    14581 tggtgatatt gaacatatgc tgacctttat gcgcgatctg catcgttata cggcgcgcaa
    14641 tatgggcgat gagcggatgt ggccgttaag tatgccatgc tacatcgcag aaggtcagga
    14701 catcgaactg gcacagtacg gcacttctaa caccggacgc tttaaaacgc tgtatcgtga
    14761 agggctgaaa aatcgctacg gcgcgctgat gcaaaccatt tccggcgtgc actacaattt
    14821 ctctttgcca atggcattct ggcaagcgaa gtgcggtgat atctcgggcg ctgatgccaa
    14881 agagaaaatt tctgcgggct atttccgcgt tatccgcaat tactatcgtt tcggttgggt
    14941 cattccttat ctgtttggtg catctccggc gatttgttct tctttcctgc aaggaaaacc
    15001 aacgtcgctg ccgtttgaga aaaccgagtg cggtatgtat tacctgccgt atgcgacctc
    15061 tcttcgtttg agcgatctcg gctataccaa taaatcgcaa agcaatcttg gtattacctt
    15121 caacgatctt tacgagtacg tagcgggcct taaacaggca atcaaaacgc catcggaaga
    15181 gtacgcgaag attggtattg agaaagacgg taagaggctg caaatcaata gcaacgtgct
    15241 gcagattgaa aacgaactgt atgcgccgat tcgtccaaaa cgcgttaccc gcagcggcga
    15301 gtcaccttct gatgcgctgt tacgtggcgg cattgaatat attgaagtgc gttcgctgga
    15361 catcaacccg ttctcgccga ttggtgtaga tgaacagcag gtgcgattcc tcgacctgtt
    15421 tatggtctgg tgtgcgctgg ctgatgcacc ggaaatgagc agtagcgaac ttgcctgtac
    15481 gcgcgttaac tggaaccggg tgatccttga aggtcgcaaa ccgggcctga cgctgggtat
    15541 cggctgcgaa accgcgcagt tcccgttact gcaggtgggt aaagatctgt tccgcgatct
    15601 gaaacgcgtc gcgcaaacgc tggatagcat taacggcggc gacgcgtatc agaaagtgtg
    15661 tgatgaactg gttgcctgct tcgataatcc cgatctgact ttctctgccc gtatcttaag
    15721 gtctatgatt gatactggta ttggcggaac aggcaaagcg tttgcagaag cctaccgtaa
    15781 tctgctgcgt gaagagccgc tggaaattct gcgcgaagag gattttgtag ccgagcgcga
    15841 ggcatctgaa cgccgtcagc aggaaatgga aaccgctgat accgaaccgt ttgcggtgtg
    15901 gctggaaaaa cacacctgac agaaaagaaa aaggccactc gtgagtggcc aaaatttcat
    15961 ctctgaattc agggatgatg ataacaaatg cgcgtctttc atatactcag actcgcctgg
    16021 gaagaaagag ttcagaaaat ttttaaaaaa attaccggag gtggctaaat gccgttgtta
    16081 gatagcttca cagtcgatca tacccggatg gaagcgcctg cagttcgggt ggcgaaaaca
    16141 atgaacaccc cgcatggcga cgcaatcacc gtgttcgatc tgcgcttctg cgtgccgaac
    16201 aaagaagtga tgccagaaag agggatccat accctggagc acctgtttgc tggttttatg
    16261 cgtaaccatc ttaacggtaa tggtgtagag attatcgata tctcgccaat gggctgccgc
    16321 accggttttt atatgagtct gattggtacg ccagatgagc agcgtgttgc tgatgcctgg
    16381 aaagcggcaa tggaagacgt gctgaaagtg caggatcaga atcagatccc ggaactgaac
    16441 gtctaccagt gtggcactta ccagatgcac tcgttgcagg aagcgcagga tattgcgcgt
    16501 agcattctgg aacgtgacgt acgcatcaac agcaacgaag aactggcact gccgaaagag
    16561 aagttgcagg aactgcacat ctagtcagta aactatcttc acaattaatt gaaaaaagcc
    16621 agttcaaatg aactggctta gttgtactta gtgcgcaccg ccgccaccgc cacctgcgcc
    16681 aaatggcggt ttagcaaacc acaccagccc cagcagtacg aggaatatcc cggctgacat
    16741 ccagaagatc tcattggcgg aaataatcag cccctgattg gtgatctgct gggcaatcca
    16801 gcctgatgcc tgctgttgcg tcatcccaag cccttccagt tgactgtaca tcgcctgggc
    16861 attcgggttg aacgggttta ccgactcagt caactgcgca tggtgcatcg actcgcggtt
    16921 ggtccacatg gtcgtggtta tcgacgtgcc gatagacccc gccagcgttc gcgtaaagtt
    16981 agagaggctc gatgccgccg ccagtcgttc cggtggcaaa ccagacagcg taatggtggt
    17041 cagcggcata aagaagcagg ccaccgcaaa cccctggata aactgcggcc aggccgacgc
    17101 gccaaaatcc atacctggtt caaaggtata ggcacgccag tagaagcaga cggcatacat
    17161 aataaagctg aaggttacca gccgccgcat atccagttta tgcgcgaagc ggccgataat
    17221 cggcgacagg atcaccggaa taatccctac cggcgcagag gccaaacctg cccaggtcgc
    17281 cgtgtaaccg tagacctcct gcaacaactg cggcagcaga acaatagcgc cgaagtagag
    17341 catatacgcg aggctgatac acaagcagcc gatggtgaag ttgcgcgact taaacaacga
    17401 cagatcgact atcgggttat cgtcggtcag ctcccagaca atcaggaagc agatagccac
    17461 caccgccacc acggtaagga tgataatttc ctgtgatgaa aaccagtcca gctctttacc
    17521 gcggtcgagc ataatctgca ggctgccgat accaataacc agcagtgcca gccccacggc
    17581 atcaatccgc cgccgtttgg tgcgggtttc acgtccgcgc agagtttgca gtgtcatcaa
    17641 caccaccgcc acgccaatcg gcacgttgat gaagaatatc cagccccagt ggtaattatc
    17701 gctgatataa ccgccgagga tcgggccgca aattggcgcg acaatcaccg tcatcgacca
    17761 caacgccagc gcgatcgagc gtttggctgg cgggtagtta ttcagcaata gactttgcga
    17821 aagcgggatc aacggcccgg cgacaatccc ctgaatcacg cggaagaaga tcagcatatt
    17881 caggctgctg gagacaccac acgcccacga cgcaatagca aaggcgatgg tggaccaaag
    17941 gaacagtttc acttccccga cgcgctttgc cagccagccg gtaagcggga tcgagatggc
    18001 attcgccacc ccgaaagaag tgattaccca cgttccctgg ctgagcgatg agcccagatt
    18061 cccggcgata gtggggatcg ccacgttagc aatggtggag tccagcacct gcatgaatgt
    18121 cgccagtgac agcgcaatcg tcataatgac cagttgcgcg ccttccagcg gtttttgctg
    18181 ttgcatcaca cgcacctctg gattagccag cgttagcttt tacgatatcg tcgatcagtt
    18241 tattgacagg tgccaggctg atttcacgcg cggtacttac cgcgaccggt gttgaacgta
    18301 ctttatttgc cagtacctga ccgtcacggt tagtggtatt gacgctcacc agtgtggaca
    18361 aaccaatgcg cagcgggtat tgctccagct gtttctggtc cagttcgata cgcacaggta
    18421 gacgctgaac gactttgatc cagttaccgg tcgcattttg cgccggaagc agtgagaatg
    18481 cgctacctgt gcccatatcc agaccaacca ctttaccggt gtatttcaca tcatcgccgt
    18541 aaatatccgt ggtgatagtg accggctgac cgatacgcat attggcaatc tgcgtctctt
    18601 taaagttggc atccacccac atattggtgg ctggaacgac cgccatcagc ggcgtcgttg
    18661 ggctaatttg cgccccaggc tgtaccgcac ggcgggagac ataaccggtc atcggactga
    18721 caatacgggt acgctccagc gccagccagg cgttacgtac ttcggtggca gcctgttgca
    18781 cggcaggctg atcttccagt ttagtcccca gaatcatcgc ctgattggca ttgtattgtt
    18841 gaatagcgac gtccagttgc gcctgggcac tggtgacggc gtcgcgggcg tgttgcagct
    18901 cttcgcgacc aatcaggttg gcattgccca gcggcacacg gcggttgtag tcgctttgtg
    18961 cttttgcgag ggcgattttc tgcacctcaa tattcgcctg caactgtttg ctgttaatca
    19021 tcagctggtg ggtttggcga acgctggaag ccagtgcagt tttggctttt tcaaacgcct
    19081 ggcgagcatc tgtcgggtcg agagtgacca gcacgtcgcc ttcttttaca aaatcggtgt
    19141 tatcggccca gactttcgtc acgctgccag acacctgaga cataatttga atctgattcc
    19201 ctgccacgta tgcgtcatcg gtttcttcga aatgacgcag taccaaaaac caataaatcc
    19261 ctatcgctac ggcaataatt ataaagagca aggtgagaag gaggagcaga cgcttacgtt
    19321 tgccgctctt ctttaccggt tgctgcgggg tttgagtctc cgcatttgcg ctcatattgt
    19381 tctccacgat cttcttaact catcggctga gccgacctgc ttgccaaaaa ggccagcatg
    19441 ttgcgatgct ggccggtcat tttttctttt ataaatctgg atttttgagc gagatgacgc
    19501 gttagctcat cgcttcgaga accacaccgt cttgttccat ctggtcgaga cgggagagca
    19561 atttgcgggt gatttgctcg agctgatctt tttctgttgt gctgagcgcg gaccagagtt
    19621 gatgcaggca gttatgctgc ggtggtaaaa cctcgcgcaa aaactcgtga cctttttccg
    19681 ttaattgcag atgcaggcag cggcgatcgt tatcgctttc acgacgttcg atccaaccgc
    19741 gtttttccag ttcatcggca atacgcgtcg cgttggtacg ggatgatcca agagcacaac
    19801 ttaattcaga aggctgaata ctgtggtttt cctgagactc cagcgtaatc aacgccataa
    19861 acaacgtctc gttaatcccc tgagccttca gcattttatt gcggttctcc agcagcttgc
    19921 tttgcatgtg catgcaaaga cgagtcagaa ggatctcctg ataaggaaaa tcttcgtggc
    19981 ggctggcgcg aaattttagc atttgttcaa tgggcgtaaa cgaactatcc atttgggtat
    20041 gacctcatta atttcagccg atatagtaac gacagtgaca aataaagtaa atgtattgtt
    20101 ttagaaaaat gattcttgtg ggttatataa tcgccatcac tttccaggcg agcccatagg
    20161 ccagcgcact aagcagtgtt gggatgataa tgctgcgtgt tttatagaaa ctggcaccca
    20221 gtaccgcgaa gccgaccagc gtgggcacga aacggcgtgt atcgtgcatc acttctggtg
    20281 cggtagagac aaccagcaga gcgcatatcg aggcgatgcc aatggtgtcg agcaaaatac
    20341 ctaccgcgcc acgtttggtt gggcgggcat tacccacacg caggcgcagc ggcaaatagc
    20401 ggaagcaata attcgccgcg ccaaccagta acccaggcag cagaacctca tagctcatcg
    20461 ggcgctcctt gccagaatgc ttggattaac gcagtgaggc agccacagac aatgcctgcc
    20521 agaatggcga cgggaataga aaatagcgtt acgcctgcaa gggcaccaac taacgctgcg
    20581 gtaacgcaaa gagattgttt gcgctggaaa gaggcgagca ggaaactcat aaagagtgcc
    20641 ggaagcataa aacctaatgc tgcttcaacg gcgggataac cttgcagcaa gccgctgcca
    20701 gagaatgccc ctattaccgt accaaatacc cacgatgacc atgaactgaa ggcaatgccg
    20761 atcatccagt tctcgctcca gcggcgatta ttgcgtacca gttttgctgt tgcggcggca
    20821 aaaacctcat ccgtcaggcc aaacgcccac agggcggttt tcgatttttg cagacgctga
    20881 ataatacggc tacgcagtga cgggccatac aacacatggc gaacatccat tgccatgacg
    20941 gtcagtgcag caacccacaa actactcccg gctgccagca tcgcggtaat gacgaactgg
    21001 ctcgcgcctg cataaatgat gcaggagaaa aaaacgcttt cgagaggaga gaatcccaga
    21061 cgggtcgcat tcagaccgaa cgcaaaggcc accggaatat aactaataac aatcggtaaa
    21121 ctgtctttgc atccttccat gaaggtcgcc gaaccaggag caggctgtgg agtagggctt
    21181 tccatagagt gtacgcttaa cgattgttaa tattataaat agactgaatg aatatcttaa
    21241 ccttatcaga ctgatgggct tcttaacacc cttataagtg taaagccacg aaaacggttg
    21301 ctgattgctt aatttgcggc ttcctgacga tgaaaacctc gccaccagac cagtaagtta
    21361 actagggcaa tcgtcgcgcc agccagacaa acgccagccc aaccgccatg ttgccaggct
    21421 gaggctgaaa ttagcgaacc ggcggcaccg ccaataaagt agctggtcat gtaacctgcg
    21481 gtcaggcgat tacgcgcatc agggtgtatt cgataaatta ccgtctggtt agtgatatgc
    21541 acgccctgta cggtgagatc cagcaccagg atgccgataa tcagcgccag tacggaagtg
    21601 tgaccaaacc agatcgccag ccatgaaagt aatagcagca gcagaccgaa agttgtggtg
    21661 tggtgcgatt tacccttatc ggcaaaaccg cccgccggac gagcgcccaa cgctccggca
    21721 gctcccgcaa gtccaaacag accaatgaca ccatcgctgt agttaaaagg tggagcggca
    21781 agcaaaaagg ccattgaggt ccagagaatg ctgaagttgg caaaggtcag gcagcccagc
    21841 aatgcgcggg tgcggaggat cttatcgctg ataaacatgc tgaaaacgga acctaacaac
    21901 tgtgggtagt tgaggtgggt ttctgatttc atttgtggca gaccacgcca taatgctagc
    21961 gccatcagtg ccattaacac cgaggcaacc caaaagacgg tgcgccagcc gccgaggttt
    22021 gccagtaacc cggcaacggt tcgtgccagc aagatcccca acagcagacc gctcataatg
    22081 gtgccaacca ctttgccgcg tttatccggt gaagccagcg tcgctgccag cggaaccaga
    22141 atttgtgcca cgactgagaa taaaccggtt aatgccgtac cgaggatcat catcgccagc
    22201 gactgactgc tggcagtgat taacatgccg cctgccgcca gtaaggtcat cgagacaatc
    22261 aggcggcggc gttcaaacat atcaccgagg ggaacaagaa acagcagacc tgctgcatag
    22321 cccaactgcg cggcggtaac aataaagcct gccgaactgg cggaaaggga aaagttacgc
    22381 gcgatggtgt cgagcaatgg ctgggcgtaa tagttactgg ctaccgcctg accggtggcg
    22441 atagacatca gcacgatcag cgccgggcta agctcatgat taggtttagt cattattttt
    22501 gttcatcagg tgatgattaa gctggaaatc aataatagcg gatgttgatg aatgggggcg
    22561 aaagatagct tgttgaaatg tgcgatgtgg tttgtaggca tgataagacg cgccagcgtc
    22621 gcatcaggca ttgtgcacag aacggcggat gcggcgaaaa cgccttatcc gccatacgga
    22681 atgagtgtat tttgtaggca tgataagacg cgccagcgtc gcatcaggca ttgtgcacag
    22741 aatggcggat gaggcaaaaa cgacttatcc gcccgaataa aaattacttc tgcgctgcca
    22801 gcgcctcatt cacccagcca tcgaactgct gctggtgggc tttgatccaa ccatcaacat
    22861 ggccctgaat atcgccttct gaggctttgc cgtcatgcat aatggcgttc tgggcgttaa
    22921 tatctgccac tggcaactgc ataatggcaa aaagtttcgc tgctgccggg tttttctcgg
    22981 cccaggcttt gttggcaacg atatgcatgg tgctgaccgg gaagccataa ttcgcaccat
    23041 tcggcagttt ggtatcggcg tttttatcgc ccggcagtgc ggagaacggc acctgcaacc
    23101 agaccacatc tttccctggc ttcagctcat tactcaccca gtacggcgtc caggtgtaat
    23161 aaaacaccgg tttgccctct ttgtagcgac tgatggtgtc ggccatcatc gctgcgtagt
    23221 tcccctgatt atgcgtcacg gtgttggtca gttcatacgc ggcaagctgg tggttgatcg
    23281 caccttcgca gccccagcct gggttacagc cggttaaatc cgctttaccg tcgccgttgg
    23341 tatcgaacag tttggcgatc ttcggatctt tcagttgtgc gatgttggtg attttgtact
    23401 ggtcggcggt tttcttatcg atcaggtaac cctgtgccgc gccgttaacg aatatccctt
    23461 cacaataaaa tttcttatcg ccaccggcag cttcgtacat gttgtcatgc agcggcgtcc
    23521 agttcacggc ggtgaaggtt gcatcgccgg aagcaagtga ggtgtagcca acgttgtaat
    23581 ccacttcgct gggtttattg acggtataac ctaatttctc cagtgcacga ctgaccagca
    23641 gcgtctggaa ggtttcttca gtgatggtgc tctgaactgg attaacagta atgcctttgc
    23701 ccggcagatc ggctgcaaaa gtttgtgtag agataagcgt ggcaaacgct gtcgcaaaaa
    23761 gtacgctatg tcgcattgtt attccttttt ttggcagggt gataatgccc ggcgttaccg
    23821 ggcaagtgca gagttactta atgaatgggc gggtcagcag accgacaggg ccagtggtgt
    23881 accagcgacg gttgccgcga ctgcgtgagt cgcgcccaac ggcctgcgtc aggcggtcga
    23941 ggataatggc gaggatcaca atcccgacgc cgccaacggt ggcaaggccc atatccagac
    24001 gaccgatacc gcgaagtacc atctgaccca gcccgccgac ggcaatcatc gaggcgatga
    24061 ccaccataga aagagccagc atcagcgtct ggttaacgcc cgccataatg gtcggcatcg
    24121 ccagcggtaa ctgaactttg aacagcatct ggcgcgggct ggcaccgaat gagcgcgagg
    24181 cttcaatcag atccgccgga acctggttaa tccccagaat cgtcagacgg ataatcggcg
    24241 gcagagcaaa gatgatcgtc accactacgc ccggcacgtt accgatacca aacagcatga
    24301 cgattggtac cagataaaca aacgctggcg tggtctgcat ggcatcaagc agtggacgaa
    24361 taattttcgc cgctcgcgga cttctcgcca gccatatccc caacggcaaa ccgatgacga
    24421 tacagaacag cagggcggtt aacaccagcg ccagagtcac cattgcctgc gaccaggcac
    24481 cgattgcgcc gatggcaatc agcgaaacca gcgtcgccac gcccattccg accccggaaa
    24541 tctgccaggc gatgagagcg aaaacgataa tcgccactgg cgctggcata cccagcagca
    24601 attgctggaa actgttgagg atataatcaa ccggaacgcg cacgccctgg aagacgggac
    24661 ggaaatgggt aacaacccag tcgatccctt cagtgaccca actgtcgagc gggatcagcg
    24721 ttttatggaa cggatcgaga atattaaaat gctcgacgtt tggcgcaggc gtactggtca
    24781 gccagtcagc accaccgccg tcagtcggtg cagtcgtcgg tgtaccccag gcgtctgcgg
    24841 attgcgcggc actgtccgcc gctggcgtgg tatcccacgg attattttga tcagccatta
    24901 tttaccccct cacgatctaa agcgcgcagc agcattcctt tcgaaatgat gccgacatac
    24961 tgttggtcct cgtcgaccac gggcaccgca cagggtgcct gtccgacatg agagagcaac
    25021 tcgctaagag gcgtttgtgc atcgactgct aacggcgcat caatcagcgc cgcatcaaga
    25081 ccttgctgct gcgttaacgc ggttttaagc gaatcgatgg agactgcgcc gacaaactta
    25141 ttaccgcgtt cgataacgta gccatattcg cgatcttcat cctgcaataa tttcagtgcc
    25201 gaacgtgggc cgaagccagg ggttttacga attaagccat tcggtgtccg gcgggcaata
    25261 tctttcgcac tgaatacctg actaatatca acgccacgga agaaggtacg gacataatca
    25321 ttcgccggat tattgagaat ttcatccggt gtgccgacct gtaccacttc accattttgc
    25381 ataatggcaa ttcggtcgcc aatacgcatg gcttcatcga gatcgtggga aataaagaca
    25441 atggtgcgct gatgtttcgc ctgtaatttt accagctcat cctgcatctc ggtgcgaatt
    25501 aatggatcga gcgccgagaa ggcttcgtcc attaataaaa tatccggatt aatcgctaac
    25561 gcgcgggcta atcccacacg ttgacgcatc ccgccagaga gttcatccgg gtagctgtgg
    25621 gcataatttt ccagcccgac ctgacgcagt gcatcaaggg ctttttcccg gcgttcttcg
    25681 gcattaattc cggccaattc cataccgaac gcagtattgt ccagcacggt catatgcggc
    25741 attaaggcaa aggactggaa gaccatcgca atcttttttc tgcgcacctc acggagtttg
    25801 gcatcagata ttttggcaat atccacacca tcaatcagca cttgcccgcg ggtgggttca
    25861 atcaggcgat tgagaaggcg taccattgtg gatttacccg agccggataa tcccatgatg
    25921 acaaatatct cgccttcttc aatggccaga ctggcgtctt ttacgccaag cgatagccca
    25981 gttttttcca gaatttgttc ttttgaaagt ccttgttcga tatatttgaa tgctcgctgt
    26041 ggatgctcac caaatatttt ataaaggttt ttaatttcta atttaattgc catgcaatag
    26101 aaagattcct gttatttgtt tatgtcgata tattacccaa tggaaatagt tacttttttc
    26161 taccctaaca tactgagaat ctgaggcaac ccctgatggc aaatgtggca tgaatattga
    26221 tgcgagcaaa tcgccggaat ttcccgtgat ataagggctg agagcaaatc gagaaaagtt
    26281 gatatttttt gtcaaagata gcgaaaaaac gcctgaatat tgtggtttca ggcgaataat
    26341 gcaacagagt atcagtgtag atcaccacaa aatatttgcg tgataaaaag ctatttggcg
    26401 ggaattattt ccctgtcgcg ggtagtgata tttttgaaaa taacaccctc agaaattcca
    26461 gtcttcatct tctgtttcaa ccgctttccc catcacataa gaggagcctg aaccggaaaa
    26521 aaaatcgtga ttttcatcgg cattcggcga aagcgcggcg aggattgccg gattcacttc
    26581 cgccatttct gcgggaaata aaggttcgta gcccagattc atcaaagcct tattggcgtt
    26641 gtaacagaga aacgctttca catcgtcagc ccacggggtt tcggcgtaca gctcatcggt
    26701 gtattgcagc tcgttgtcgt agagttccag caacaaatcg aaggcgaaac tcttcaactc
    26761 ttcacgttgt cccagagata tcttttccat gtttttctga tatttatagc ctatgtagta
    26821 accgtggact gcttcatcac ggataatcag acggatcagg tctgcggtat tggtcagctt
    26881 tccgcggctg gaaaaataca tcggcagcca gaaaccggaa tagaacaaaa aagattcaag
    26941 aaacacactg gcgattttct ttttcagcgg atcatcaccg cgataatgtt gctgaataat
    27001 ctgagctttt cgctgcaacg gtgcgttttc ttcactccag gcgtaggcgg catcgacatc
    27061 tttggtctgg cacagcgtcg agaaaatcga actgtaagag cgggcatgaa ccgcttccat
    27121 aaagctgata ttcgataata ccgcttcttc atgaggcgtg agtgcatcgg gcatcagcga
    27181 aggcgcgccg ataacatttt gcagcgtgtc gagcagcgtc aggccagtaa aaacgcgcat
    27241 cgtcagttgt tgttctacga cagttaatgt ctgccaggca ggaatatcgt tcgacagcgg
    27301 caccttttct ggtagccaga aattgctggt caggcgattc cacacctcca gatctttatc
    27361 gtcagatatc ttgttccagt tgatggcgct gatacgtgag agtttcatag atattcctta
    27421 aagtgcacag gagacgcagc cttcaatttc agtgccttcc agcgccatct gacgcaggcg
    27481 aatgtaatag agcgttttga tacctttgcg ccaggcgtaa atctgcgctt tgttgatatc
    27541 gcgagtggtg gcggtatcgg ggaaaaacag cgtcagcgac aaaccctgat cgacatggcg
    27601 agtcgcttcc gcgtaggtgt cgatgatctt ttctgcgccg atttcgtaag catcctgata
    27661 cagcgccaga ttttcgttag tcataaacgg ggcagggtag taaacgcgtc ctgttttgcc
    27721 ctctttgcgt atctctactt tcgccacaat cggatgaata ctcgacgtag catggttgat
    27781 ataagagata gaaccggttg gcggcaccgc ctgaagattc tggttgtaaa tgccgtagcg
    27841 catcacgtcg tcgcgcagtt gcgcccacat ctcacgggta ggtaacgtaa taccgctgcg
    27901 ggcaaacagt tcgccaactt tcgccgtttt cggctgccag ttaccttgca gatattggct
    27961 aaaatattca ccactggcat aacgtgactg tttgaacccg gcgaaggttt caccgcgttc
    28021 gcgcgccagc aacatcgagg tacgcagtgc gtgccaggtg atggcataga aatagagatt
    28081 ggtgaaatcc agtgcttccg gcgaaccata agcgatgcct tctcgcgcca gatagccgtg
    28141 taaattcatc tgccccagtc cgatggcgtg tgaggcggca tttccggctt cgatggacgg
    28201 cacgctgcgg atatgactca tatctgacac cgccgttaaa ccgcgcacgg cagtctctac
    28261 cgtgcgggca aaatcggggg aatccatggt gtgcgcaata ttcaacgaac ctaaattgca
    28321 ggagatatca tggcctgtgc gggcatagtc gagattctcg tcatactctg aggcacagtt
    28381 gacctgcaaa atctctgagc agagattact catatttatg cgcccggcga cagggttagc
    28441 acggtttacc gtgtcttcat acatgatgta gggatagccg gactcaaact ggatttctgc
    28501 cagccgctgg aagaaatcac gggcgttgag gtattttttg cggatgcgtt catcggcaac
    28561 cagttcgtca tagtgttcgc tgatggcgat atcggcaaac ggcttgccat aaactcgctc
    28621 aacgtcatag ggcgaaaaca gcgccatctg cgcattctct tttgccagat ggaaagtgat
    28681 atccgggatc accacgccca gcgacagtgt tttaatgcgg attttttcgt cggcattttc
    28741 ccgttttgta tcgagaaaac gcagaatatc gggatgatga gcatgtaaat agactgcacc
    28801 tgccccctga cgcgcgccga gttggttggc ataggaaaat gcgtcttcca gcattttcat
    28861 caccggaatt acgccagaag attgattttc aatacgttta attggcgcgc ccgcttctcg
    28921 cagattcgac agcaaaaatg ccacgccgcc gccgcgtttc gacagttgca gtgcggaatt
    28981 taccgcccgg ccaatcgact ccatattgtc ttcaatacgc agcagaaaac aggaaaccag
    29041 ttcgccgcgc tgctgcttac cgcagttgag gaatgtcggc gtggctggct gaaagcgtcc
    29101 tgacagcatt tcattggtca gttgcaacgc cagcgtcgca tcgccttgtg ccagcgtcag
    29161 cgccaccatc gtgactcgat cggcaaaatc ttccagataa cgtttaccgt cgaatgtctt
    29221 caacgtatag ctggtgtaaa acttccatgc cccgaggaat gtctggaaac gaaaaccgct
    29281 ggtgtgtgcg tgggcaaaca gcgtaatgac aaagtcgcga gagtagcgat taagaacgct
    29341 ttcatcatag taaccttcgt tgaccagcca gttcaggcgc tgctgctggc tactgaaggt
    29401 cacactgttc ggacgcacat gcgtcgccat aaaggcgtca acggcctggc ggtctttatc
    29461 gaactgaatg cgacctgcgc tatcgtagag gttaagcatc gcattcagcg cgtggtaatc
    29521 catcgtttcc tgcgtcaggc attctgcggt tgtcgttgcc aaaattcggt tactccttta
    29581 cgaacgtttt cgatatcgct ttgcgtaccc atgagttcaa aacggtacag ccacggcacg
    29641 ccgcatttcc gggcaatcac atctccggcg cggccatacg cctcaccaaa gttgcgatta
    29701 ccagaagcaa taacgccgcg aagcaacgcc cggttgtgct cgtcgtttaa aaagcgaatt
    29761 acctatcgtg gcaccgcgcc agccgtaccg ccgccgccgt aagagggcac gatcaggatg
    29821 taaggctcgt ctacctgaat ccgttcccgc tcattgagcg ggatgcgcac cgcgggcaga
    29881 cctaaacgtt cgataaaacg ctgcgtgttt tcggagctgc tggagaagta gacgagctgg
    29941 ctcatgcact ggccgcgtgt ggcgctggat gcagacggtt aatcatgtcc ggacggaaac
    30001 cagaccagct aagatcgcca gcaatcacta ccggcaactg acgaaagccc tgagcacgca
    30061 acgcttccgc tgcttcagga acgcgatcga cattaatcat ttcaaaatca aagccccggt
    30121 tttccatcgc ccgtttggtg gcgtggcact gaacgcaatc gttacgagtg taaatagtta
    30181 tgcgcatgat tcgtatttcc gtttaaaatg aagatacggc gcgatgatac gcgtcgggtt
    30241 gtctctctgt tgatacagag atactagatg tagttgaaaa aagattcaac cacacaatat
    30301 atagcaaata gtgatcgaaa ttaccctaga tatgagcgtg acgggggagg gggatttttg
    30361 tgattcacca ggcaaaaaga aaccccgaag acaggcttcg gggttaaaga cgcgtattta
    30421 ttatcatttt tgcactacga tttgcgcatg cttaacagtg cgccgataaa aatacctacc
    30481 gcagctgctg tacccacgct acaccagggt ctttcacgaa caaaagaatc cgcgcagcca
    30541 acggcatcgc gtgcggcttg ctggacgcga gtacgaccat gcattcgcgc cctggtttct
    30601 ttcagcaatg cctgagcttt actgcgtgcg gcttcagctt cccctttggc gtcgctgccc
    30661 caggacttca atacagattc gaggctgtca gctaattgat tgacatcatt ctggatatcc
    30721 tgcacgccat catcaacgtc gttgcggttc ggtcggttaa acatatgatc ccccattaga
    30781 attcgatgta attttcagtg tagaccataa ttttcttaac tgaggttcat cgcggcttta
    30841 tggacttttc tgaaagcgtt gtgaatgcgc aatcaggtaa ggtaggcccg cggttgataa
    30901 taacgaggat caaatatgta tttacgacca gacgaggtgg cgcgcgtact tgaaaaagtc
    30961 ggttttactg tcgatgtggt aacccagaaa gcttatggtt accgccgtgg ggaaaattat
    31021 gtctatgtta atcgcgaagc gcgaatggga cgtaccgcac tggtaattca tccaacatta
    31081 aaagaacgta gttcgacact ggctgaaccc gcttccgata ttaaaacttg cgatcattat
    31141 caacaatttc cgctctattt agcaggcgag cgacacgagc attacggtat cccgcatggc
    31201 ttcagttcgc gtgttgcgct tgaacgttat ttgaatggtt tat
//
LOCUS       DAZPAC010000027        30143 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZPAC010000027 DAZPAC010000000
VERSION     DAZPAC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30143
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.027"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..221
                     /gene="tuf"
                     /locus_tag="ACHUPM_001027"
     CDS             <1..221
                     /gene="tuf"
                     /locus_tag="ACHUPM_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR0868875.1"
                     /translation="FFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHP
                     IAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            451..834
                     /gene="secE"
                     /locus_tag="ACHUPM_001028"
     CDS             451..834
                     /gene="secE"
                     /locus_tag="ACHUPM_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR0868876.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            836..1381
                     /gene="nusG"
                     /locus_tag="ACHUPM_001029"
     CDS             836..1381
                     /gene="nusG"
                     /locus_tag="ACHUPM_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR0868877.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1540..1968
                     /gene="rplK"
                     /locus_tag="ACHUPM_001030"
     CDS             1540..1968
                     /gene="rplK"
                     /locus_tag="ACHUPM_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR0868878.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1972..2676
                     /gene="rplA"
                     /locus_tag="ACHUPM_001031"
     CDS             1972..2676
                     /gene="rplA"
                     /locus_tag="ACHUPM_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR0868879.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3089..3586
                     /gene="rplJ"
                     /locus_tag="ACHUPM_001032"
     CDS             3089..3586
                     /gene="rplJ"
                     /locus_tag="ACHUPM_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR0868880.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3653..4018
                     /gene="rplL"
                     /locus_tag="ACHUPM_001033"
     CDS             3653..4018
                     /gene="rplL"
                     /locus_tag="ACHUPM_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR0868881.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4338..8366
                     /gene="rpoB"
                     /locus_tag="ACHUPM_001034"
     CDS             4338..8366
                     /gene="rpoB"
                     /locus_tag="ACHUPM_001034"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR0868882.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8443..12666
                     /gene="rpoC"
                     /locus_tag="ACHUPM_001035"
     CDS             8443..12666
                     /gene="rpoC"
                     /locus_tag="ACHUPM_001035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR0868883.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12908..14041)
                     /gene="thiH"
                     /locus_tag="ACHUPM_001036"
     CDS             complement(12908..14041)
                     /gene="thiH"
                     /locus_tag="ACHUPM_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR0868884.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14038..14808)
                     /gene="thiG"
                     /locus_tag="ACHUPM_001037"
     CDS             complement(14038..14808)
                     /gene="thiG"
                     /locus_tag="ACHUPM_001037"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR0868885.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14810..15010)
                     /gene="thiS"
                     /locus_tag="ACHUPM_001038"
     CDS             complement(14810..15010)
                     /gene="thiS"
                     /locus_tag="ACHUPM_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR0868886.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14994..15749)
                     /gene="thiF"
                     /locus_tag="ACHUPM_001039"
     CDS             complement(14994..15749)
                     /gene="thiF"
                     /locus_tag="ACHUPM_001039"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR0868887.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15742..16377)
                     /gene="thiE"
                     /locus_tag="ACHUPM_001040"
     CDS             complement(15742..16377)
                     /gene="thiE"
                     /locus_tag="ACHUPM_001040"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR0868888.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16377..18272)
                     /gene="thiC"
                     /locus_tag="ACHUPM_001041"
     CDS             complement(16377..18272)
                     /gene="thiC"
                     /locus_tag="ACHUPM_001041"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR0868889.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18353..18453)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18506..18982)
                     /gene="rsd"
                     /locus_tag="ACHUPM_001042"
     CDS             complement(18506..18982)
                     /gene="rsd"
                     /locus_tag="ACHUPM_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR0868890.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19077..19850
                     /gene="nudC"
                     /locus_tag="ACHUPM_001043"
     CDS             19077..19850
                     /gene="nudC"
                     /locus_tag="ACHUPM_001043"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR0868891.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19890..20954
                     /gene="hemE"
                     /locus_tag="ACHUPM_001044"
     CDS             19890..20954
                     /gene="hemE"
                     /locus_tag="ACHUPM_001044"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR0868892.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20964..21635
                     /gene="nfi"
                     /locus_tag="ACHUPM_001045"
     CDS             20964..21635
                     /gene="nfi"
                     /locus_tag="ACHUPM_001045"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR0868893.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21678..22268
                     /locus_tag="ACHUPM_001046"
     CDS             21678..22268
                     /locus_tag="ACHUPM_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR0868894.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22455..22727
                     /gene="hupA"
                     /locus_tag="ACHUPM_001047"
     CDS             22455..22727
                     /gene="hupA"
                     /locus_tag="ACHUPM_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR0868895.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22740..23435
                     /locus_tag="ACHUPM_001048"
     CDS             22740..23435
                     /locus_tag="ACHUPM_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR0868896.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23437..23862)
                     /gene="zraP"
                     /locus_tag="ACHUPM_001049"
     CDS             complement(23437..23862)
                     /gene="zraP"
                     /locus_tag="ACHUPM_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR0868897.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24100..25476
                     /gene="zraS"
                     /locus_tag="ACHUPM_001050"
     CDS             24100..25476
                     /gene="zraS"
                     /locus_tag="ACHUPM_001050"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR0868898.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25473..26798
                     /gene="zraR"
                     /locus_tag="ACHUPM_001051"
     CDS             25473..26798
                     /gene="zraR"
                     /locus_tag="ACHUPM_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR0868899.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26795..28084)
                     /gene="purD"
                     /locus_tag="ACHUPM_001052"
     CDS             complement(26795..28084)
                     /gene="purD"
                     /locus_tag="ACHUPM_001052"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR0868900.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28096..29685)
                     /gene="purH"
                     /locus_tag="ACHUPM_001053"
     CDS             complement(28096..29685)
                     /gene="purH"
                     /locus_tag="ACHUPM_001053"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR0868901.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7056 a         7860 c         8305 g         6922 t
ORIGIN      
        1 cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg actggtacca
       61 tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa atggttgtta
      121 ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt gaaggcggcc
      181 gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata acatttgacg
      241 caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt accagaacct
      301 ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt tgagggcgta
      361 taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg gggtgaaaat
      421 gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc tcaaggaagc
      481 gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct ggtggcgatt
      541 gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc cgtagtaatt
      601 ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc taccgttgct
      661 tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg ccaggaaaca
      721 ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat cctgtgggga
      781 ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt ctgagatgtc
      841 tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg aaggccgcgt
      901 agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt ttggtgaagt
      961 catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca aaagcgaacg
     1021 taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga gctggcacct
     1081 ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc gtcctgcgcc
     1141 aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg gtgataagcc
     1201 gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg gtccgttcgc
     1261 tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga aagtgtctgt
     1321 ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg aaaaagccta
     1381 acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat tggaatacaa
     1441 tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata tcacggggag
     1501 cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa agtacaagcc
     1561 tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt aggtccggct
     1621 ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc aaaaactgat
     1681 tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga ccgttctttc
     1741 actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc tggtatcaag
     1801 tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc tcagctgcag
     1861 gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc gatgactcgc
     1921 tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga aatggctaaa
     1981 ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca gtacgacatc
     2041 aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga aagcgtggac
     2101 gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg tggtgcaact
     2161 gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca aggtgcaaac
     2221 gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct ggctgaccag
     2281 atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc aatgcgcgtt
     2341 gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc gaaagtgggt
     2401 actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca ggttcgttac
     2461 cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt tgacgctgac
     2521 aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa accgactcag
     2581 gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc aggtgttgca
     2641 gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg cggtgatttt
     2701 gtctacaatc ttacccccac atataatgct taatgcagac gtatatccga gatattcggg
     2761 ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg tgactggtgc
     2821 gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa gatttgttcg
     2881 ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca agaaacttaa
     2941 tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg gcttgtttct
     3001 gctcaccgta attaagacgc tctctccgct tggaggagtg aagtgagttc cagagatttt
     3061 ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca aacaagcgat
     3121 tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg cggattcccg
     3181 tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag ctggcgtata
     3241 catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc cgttcgagtg
     3301 cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac acccgggcgc
     3361 tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg tcaaagccgc
     3421 tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa ctctgccgac
     3481 ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg ctggtaaact
     3541 ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc agttatcttt
     3601 ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt aaatgtctat
     3661 cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg ttgtagaact
     3721 gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag ctgtagctgc
     3781 tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga aagctgctgg
     3841 cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg gtctgaaaga
     3901 agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga gcaaagacga
     3961 cgcagaagca ctgaaaaaat ccctggaaga agctggcgct gaagttgaag ttaaataagc
     4021 caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt ttggtcacca
     4081 gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact gctgtgcctt
     4141 tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt tctgtgtaaa
     4201 tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt tccattttcc
     4261 ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt cgacttgtca
     4321 gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt cgtaaggatt
     4381 ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag cttgactcgt
     4441 ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct gctttccgtt
     4501 ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc agctaccgcc
     4561 ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc tattccgcac
     4621 cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa ggcaccgtaa
     4681 aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca gacaacggta
     4741 cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt agtccgggcg
     4801 tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg tataacgcgc
     4861 gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag gacaacctgt
     4921 tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc gccctgaact
     4981 acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa atccgtgata
     5041 acaagctgca gatggagctg gtgccggaac gcctgcgtgg tgaaactgca tctttcgaca
     5101 tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg cgccacattc
     5161 gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac atcgcaggta
     5221 aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc gcagcgaaca
     5281 tggagctgag cctggatctg ttggctaagc tgagccagtc tggtcacaag cgtattgaaa
     5341 cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta cgtgtcgacc
     5401 caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc cctggcgagc
     5461 cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc gaagaccgtt
     5521 atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc gaagaaatcg
     5581 aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag ctcatcgata
     5641 tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt cgtatccgtt
     5701 ctgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta gagcgtgcgg
     5761 tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggac atgatcaacg
     5821 ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg tctcagttta
     5881 tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc gcactcggcc
     5941 cgggtggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac ccgactcatt
     6001 acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg atcaactctt
     6061 tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat cgtaaggtga
     6121 ccgacggtgt tgtaactgac gaaattcact acctttctgc tatcgaagaa ggcaactacg
     6181 ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa gacctggtaa
     6241 cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac tacatggacg
     6301 tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg gaacacgatg
     6361 acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg actctgcgcg
     6421 ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac tccggtgtaa
     6481 ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt atcgttatca
     6541 aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac aacctgacca
     6601 aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg tctctgggtg
     6661 aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc ggtgaactgg
     6721 cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac ttcgaagact
     6781 ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc cacattcagg
     6841 aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc gctgacatcc
     6901 cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt tacattggtg
     6961 cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt gaaactcagc
     7021 tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct gacgttaaag
     7081 actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt caggtcttta
     7141 ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg cagctcaaac
     7201 aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtttg ttcagccgta
     7261 tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa ctgccgcgcg
     7321 atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg gaacagctgg
     7381 ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg aaacgccgca
     7441 aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag gtgtatctgg
     7501 cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt aacaagggtg
     7561 taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt acgccggtag
     7621 acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag atcctcgaaa
     7681 cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg ctgaaacagc
     7741 agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg ggcgctgacg
     7801 ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt ctggctgaaa
     7861 acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa gaagcagaaa
     7921 ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc ctgtacgatg
     7981 gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac atgctgaaac
     8041 tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac agcctggtta
     8101 ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg gagatggaag
     8161 tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc accgttaagt
     8221 ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc aaccatcaga
     8281 tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt tcgctgggta
     8341 tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct gtcggggtaa
     8401 aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga tttattaaag
     8461 tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc tctggcttcg
     8521 ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac catcaactac
     8581 cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg gccggtaaaa
     8641 gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt catctgtgag
     8701 aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg ccacatcgaa
     8761 ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg tatcggtctg
     8821 ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc ctatgtggtt
     8881 atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga gcagtatctg
     8941 gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga agcaatccag
     9001 gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga agagctgaac
     9061 gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact gctggaagcg
     9121 ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc ggtactgccg
     9181 ccagatctgc gtccactggt tccgctggat ggtggtcgtt tcgcgacttc tgacctgaac
     9241 gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct ggatctggct
     9301 gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt agacgccctg
     9361 ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc tctgaaatct
     9421 ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct cggtaagcgt
     9481 gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct gcatcagtgc
     9541 ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg caagctggaa
     9601 ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg cgaagaagct
     9661 gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct gaaccgtgca
     9721 ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga aggtaaagct
     9781 atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg tgaccagatg
     9841 gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct gatgatgtct
     9901 accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc tcaggacgtt
     9961 gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga aggcatggtg
    10021 ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc tctgcatgcg
    10081 cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt agtagcgaaa
    10141 accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt accgaaaggt
    10201 ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa aatgctgaac
    10261 acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca gatcatgtac
    10321 accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga catggtcatc
    10381 ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga aattcaggag
    10441 cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat cgatatctgg
    10501 gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac tgaaaccgtg
    10561 attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta catgatggcc
    10621 gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat gcgtggtctg
    10681 atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt ccgtgaaggt
    10741 ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct ggcggatacc
    10801 gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt ggcgcaggac
    10861 ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac tccggttatc
    10921 gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt aactgctgaa
    10981 gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct gctgcacgaa
    11041 cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg ttctgttgta
    11101 tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct ggcgcgtggc
    11161 cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat cggtgaaccg
    11221 ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg tgcggctgct
    11281 gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt gaagtcggtt
    11341 gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa actgatcgac
    11401 gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact ggcgaaaggc
    11461 gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca caccatgccg
    11521 gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg ccagaccatt
    11581 actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga ttccgcagaa
    11641 cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc tcagggtaac
    11701 gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg taaagcgatt
    11761 gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg tattccgcag
    11821 gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga cctgttcgaa
    11881 gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt ttccttcggt
    11941 aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag cgatccgtac
    12001 gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg tgtagaacgt
    12061 ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct gcgtggtgtt
    12121 catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct gcagggcgtt
    12181 aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa agctaccatc
    12241 gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc tcgcgtcaag
    12301 atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc ccgcgatctg
    12361 ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc atcgttccag
    12421 gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga actgcgcggc
    12481 ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta cgcgtaccac
    12541 caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca ggtgactgca
    12601 gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg ttctgataac
    12661 gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt tttatgggtg
    12721 ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga aacgaggaag
    12781 aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa acacaacgct
    12841 ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta cgttcttaca
    12901 ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct ttccataccg
    12961 gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga tcgtcgtgcg
    13021 gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc gttttcgaga
    13081 aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac ggtgattccc
    13141 gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag atggtttgca
    13201 ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac gggcgcaggc
    13261 gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat agcaaatgtt
    13321 cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc gcgccgaggc
    13381 ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc cagaagaagt
    13441 cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat gtctcctgat
    13501 aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc tccgccagcg
    13561 gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga tgacgacgaa
    13621 agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga tgttcaaagc
    13681 ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc gttttgcgct
    13741 tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa agattggaaa
    13801 gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc agacgctgcg
    13861 cccgttgggc cagtggttcc agatagccac tggcggctgg cgataacagc gccatcatat
    13921 cgtcgcgggt gagttgcgag gcatttagcg cccgttctac gtcagcagcc gttttgccgt
    13981 tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg aaggttttca
    14041 tgccgatgcc tccagaaatc cagtcagcgg gctggtggca tgagcaaaat gactgcggct
    14101 gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa atgccttcgc
    14161 catgttgacg ggatcgtccg ctacggcaat cgccgtattc actaacaccg cgtcggcccc
    14221 catttccagg gcctgcgcgg catggctggg aacgccgatg ccagcatcga caaccaccgg
    14281 aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc cctgattcga
    14341 gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca gacgtttaca
    14401 caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta ccagcatttc
    14461 ggcggctttc agagtttcga tgggatcggg caacagccag cgggcgtcag ggtgaatctc
    14521 taattttaac cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga aaatggcttc
    14581 ttccgctgtt ttcgccccgg aagtatttgg cagcagggtc acacccgccg cgataagcgg
    14641 ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca gtgtcaccag
    14701 ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga atttccctgt
    14761 gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct caaccccctg
    14821 caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc gtccactgct
    14881 cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt tggtcgagtt
    14941 gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga tcgttaaaca
    15001 ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa cgccaggctg
    15061 cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt ctctataccg
    15121 cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac caccgggcca
    15181 accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca caggcagcgg
    15241 taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa tccgaccgcg
    15301 ctggcggtga taagcggcgt gttgagtacc acgcaggcgg cattaatctc ctggcgagtc
    15361 gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc ttttaacgcc
    15421 tcacccgtta accgttgttg taatgctgtc agttgaatat cgggattcaa ctgtgtcagt
    15481 cgctgatggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa gaggatttgt
    15541 cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt cccgacgcca
    15601 gcccccgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat aatcagcacc
    15661 tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag aatttggcgg
    15721 ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca gcaactgtgc
    15781 cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga tactgccgac
    15841 acccgttgct atcaccgcag gcgcgcgcgc cagactgata ccgccaatcg ccacggtggg
    15901 ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg gtgcagaagg
    15961 catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg ggcgtgctgc
    16021 cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca ggcctgccgc
    16081 gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga cgccatacgc
    16141 ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt tatagcggcg
    16201 gcccagcgca attgccgcca cgacatcggc ttccacctct tcattgcgcc gatctttgat
    16261 gcgtagctgg agggtacgta cgcctgcatc caacagacgt tcgatccact gtacgctgtc
    16321 caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct gatacatcac
    16381 gcttcctcct tacgcaggta gatttctccg cctctggcgc ggaagttctc cgacatatcc
    16441 gccattccca cttcaatggt ttgcgcggcg gcgtaatcac gtacttcctg gctgattttc
    16501 atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc tgactcttgc
    16561 ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag attaaactgg
    16621 tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat ttgcgcgcct
    16681 ggatgccctt tcgccaggtc agcggcgtgg acggcaatct tataggtgat aagcccctgc
    16741 ttaacatctt ctttattagg cagacccaga tgctcttttg gcgttacgta acagagcatc
    16801 gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg gtcatagccc
    16861 ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg gcagtgctct
    16921 aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg gccttcaatc
    16981 atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt atgcagctcg
    17041 gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc gtcgcccagc
    17101 gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg ttgatagagg
    17161 aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc gcgagagacg
    17221 ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc cgcatggata
    17281 gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa cgcttcccag
    17341 gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg cactgtaccg
    17401 atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata gcgaccggtg
    17461 gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt ttccacttct
    17521 tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt taccaggaaa
    17581 ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg gataatcgca
    17641 cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg cagatgtgcg
    17701 ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg ctcgcggccc
    17761 atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg gcgggcgtag
    17821 tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa gccgctaaaa
    17881 cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga actgcgcacg
    17941 gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt tgccagccct
    18001 tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc gtagaccgga
    18061 atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag cgtcgggcta
    18121 agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat ataaatgcgt
    18181 tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc ccgggcgcgt
    18241 tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtc aaggacgtgg
    18301 cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg atagcagatt
    18361 aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc gaataaacgg
    18421 tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg cgttgaatgt
    18481 aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg gcgtcaagca
    18541 ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg ccgatgtcag
    18601 ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc aggctggaat
    18661 cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc ttcgcggcgc
    18721 gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata ctgaaatgtc
    18781 cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt tcgtttagcc
    18841 tcatgtacga ttctttgcca ggcttaatgc caaccagatt gtagtaagcc acgagcagat
    18901 gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg cgctccgtca
    18961 ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa actgctcagt
    19021 gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca agagctatgg
    19081 atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa caaaaattat
    19141 ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt gtgggtcagc
    19201 gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttagtacaa cagcagcggc
    19261 gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg tttcaactgg
    19321 ccggacgagg cgtacaactg gcggagtttt accgatcgca taaatactgt ggttactgcg
    19381 ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat tgccgtgagc
    19441 gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat gattcgatcc
    19501 tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc ggattcgtcg
    19561 aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag agcggaatta
    19621 aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctcag tctttaatga
    19681 ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa gaattgctcg
    19741 aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc accgtagcgc
    19801 gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga tgatacactg
    19861 accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat cgttatctgc
    19921 gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc caggcgggtc
    19981 gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg tcgctgtgca
    20041 aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac ccgctggatg
    20101 cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta gggctctatt
    20161 ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc gacgtcgata
    20221 aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg gtgcgtacca
    20281 ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt ttccggcagc ccgtggacgc
    20341 tggcgaccta catggtggaa ggcggcagca gcaaagcgtt caccgtgatc aaaaaaatga
    20401 tgtatgccga tccgcaggcg ctgcacgctc tactcgataa actggcgaaa agcgtcactt
    20461 tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc gacacctggg
    20521 gcggtgtgct taccgggcgc gattatcaac agttctcgct ctattacatg cataaaattg
    20581 ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg tttaccaaag
    20641 gcggcggaca gtggctggaa gcgatggcag aaaccggttg cgatgcgttg ggcctcgact
    20701 ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg ttgcagggta
    20761 atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa gtagcgacta
    20821 tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac ggcattcatc
    20881 aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga ctgtctgaac
    20941 aatatcaccg ctaaggagtg attatggatc tcgcgtcatt acgcgctcaa caaatcgaac
    21001 tggcttcttc tgtgatccgc gaggatcgac tcgataaaga tccaccggat ctgatcgccg
    21061 gagtcgatgt cgggtttgag cagggcggag aagtgacgcg agcggcgatg gtgctgctga
    21121 aatatccctc gcttgagctg gtggagtata aagttgcccg catcgccacc accatgcctt
    21181 acattccagg ttttctttcc ttccgcgaat atcctgcgct gctggcagcg tgggagatgc
    21241 tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg gatctcgcat cctcgccgtc
    21301 ttggcgtcgc tagccatttt ggtttgatgg tggatgtgcc aacgatcggc gtggcgaaaa
    21361 aacggctctg cggtaaattc gaaccgctct ccagcgaacc gggcgcgctg gccccactga
    21421 tggataaagg cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt aacccgttgt
    21481 ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta caacgctgca
    21541 tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc tcggaacgtc
    21601 cggcgttcgt gcgctataca gcaaatcagc cctaattcag gtaaactgcg gataatttcc
    21661 gtatttgaga actcaacatg ttacaaaacc caattcatct gcgtctggag cgcctggaaa
    21721 gctggcagca tgtcactttc atggcttgct tatgcgaacg catgtacccc aattacgcca
    21781 tgttctgcca gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt ctcgatctca
    21841 tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa ctggagaaat
    21901 ttgaagaagc gattccttca gccgacgatt tcgatctgta cggcgtttat ccggcaatcg
    21961 atgcctgcgt ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa acgctcgaac
    22021 acgcggtgga agtgagtaag acctccatca cgaccgttgc gatgctggaa atgactcagg
    22081 ctggtcgcga aatgagcgat gaagagctta aagaaaaccc agctgtagag caagaatggg
    22141 acattcagtg ggaaatattc cgacttttag ctgagtgcga agaacgcgat atcgagctga
    22201 taaaaggcct tagggcagac ctgcgtgagg cgggtgagag caatattggt ataatttttc
    22261 agcaataaga ccagaaaacg tgatttaacg cctgatttgt cgtacctgga gtcttccctt
    22321 tcgccccccg tctggtctac atttgggggg cgaaaaaaag tggctatcgg tgcgtgtatg
    22381 caggagagtg ctattctggc atttccgtcg cactcgatgc ttagcaagcg ataaacacat
    22441 tgtaaggata acttatgaac aagactcaac tgattgatgt aattgcagag aaagcagaac
    22501 tgtccaaaac tcaggctaaa gctgctctgg agtccactct ggctgcaatt actgagtctc
    22561 tgaaagaagg cgatgctgta caactggttg gtttcggtac cttcaaagtg aaccaccgcg
    22621 ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc gcagctaacg
    22681 taccggcatt tgtttctggc aaggcactga aagacgcagt taagtaagat tgcgtggcag
    22741 tgaacagttt taacgaaggg gtggtttcac cccttttgtc tttctggcgt cgatcattga
    22801 tgctggctgg cgctctgctt ctcactgcct gtagtcataa ctcttcactt cctcccttta
    22861 ccgccagtgg atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa gacagcggcg
    22921 ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc acgacgcgag
    22981 agtatcgctg gcagggcgat aacctcacgc tcatcaatat caatgtttac agcaaaccgc
    23041 cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct gagctttatg caacgtgaat
    23101 ccgatgggga aaagcagcag ctttctaacg accaaatcga tttataccgt tatcgtgctg
    23161 atcagatccg ccagattagc gatgccttac gtcaggggag agtcgtgctg cgccaggggc
    23221 gctggcatgc gatggaacag accgtgacca cctgcgaagg gcaaaccatt aaacctgatt
    23281 tagattcgca ggcgatagcg catatcgagc gccgccagag ccgctcttct gttgatgtca
    23341 gcgtggcatg gctggaagcg cccgaaggtt cgcaattact gttagtggca aactctgatt
    23401 tctgtcgctg gcaacccaac gagaaaacgt tctgatttac cagtggccca tacccatatg
    23461 accgccacca ccgcagccgc cgtagcccat gcccattccg gctccgcgcg gaatacccgc
    23521 ttcagccatc gcgatatctc gtttcacccg taactcatct aacgactgac gcaaattctc
    23581 catctctttg gcgaccgcgt taattttgct gctatccggt gggttcgcgg ctaacagggc
    23641 attgtattca taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt gagcgtaaaa
    23701 gtcattatgg attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg cggcattttg
    23761 ctgccacata ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg ccattgctga
    23821 aagcgccatc attaccaggg caattttcgt gttccgtttc atggttaatc ctccagtggt
    23881 tgtcttactt cgggtattgc atcttccgtg ccaacgatga aacgctgata tgacgggtaa
    23941 tctggcatga taaacgagta aaaatgactc gcctgctgcg ggtagcgagt catttttact
    24001 cattgaaact taagcctttg tgttacagcg cagggtaagc gctgataaaa gatggcatga
    24061 tttctgctgt cagaaaggga tgagcaggca aagaagaaga tgcgttttat gcaacgttct
    24121 aaagactcct tagctaaatg gttaagcgcg atcctccccg tggtcattgt tgggctggta
    24181 gggttgtttg cggtgacggt gattcgcgat tatgggcgcg agactgccgc cgccagacaa
    24241 acgctgctgg aaaaaggcag tgtacttatt cgcgctcttg aatccggctc gcgcgtcggc
    24301 atggggatgc gcatgcatca tgcgcagcag caggccttac tggaagaaat ggccgggcag
    24361 cctggagtac gttggtttgc ggtcacggat gaacaaggaa caatcgtgat gcatagcaac
    24421 tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt acatccggga
    24481 gatgaagaag cgtggcggcg gatcgatagc gcagacggcg agcctgttct ggaaatttat
    24541 cgccagtttc aaccgatgtt tgctgctgga atgcaccgga tgcgccatat gcagcagtat
    24601 gccgcgacac cacaagcaat tttcatcgct ttcgacgcca gtaatattgt gagtgccgaa
    24661 gatcgtgagc agagaaacac cctgattatc ctcttcgccc tggcgacggt cttgctggca
    24721 agcgtattgt cattcttctg gtatcgccgc tatttgcgct cgcgccagct tctacaagat
    24781 gaaatgaagc gcaaagagaa gctggtggcg ctggggcatc ttgcggcagg cgttgcccac
    24841 gaaatccgta acccactttc ctcgattaaa ggactggcga aatactttgc cgagcgcgcg
    24901 cctgcagggg gagaagcgca tcaactggcg caggtgatgg cgaaagaggc cgaccgttta
    24961 aaccgcgtgg taagcgagct gctggaactg gttaagccaa cgcatctggc tttgcaggcg
    25021 gtggatctca acacgctgat taaccactca ttacagctgg taagccagga tgcgaacagc
    25081 cgggagatcc agttacgctt taccgccaac gacacattac cggaaattca ggccgatccg
    25141 gacaggctga ctcaggtcct gttgaatctc tatctcaatg ctattcaggc gattggtcag
    25201 catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat cagcgttacc
    25261 gacagcggta agggaattgc ggcagatcag cttgatgcca tcttcactcc gtacttcacc
    25321 actaaagccg aaggcaccgg actggggctg gcggtcgtgc ataatattgt tgaacaacac
    25381 ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac cctctggctt
    25441 ccggtcacta ttacgcgtaa gggcccacaa ggatgacgca cgataatatc gatattctgg
    25501 tggtggatga tgacattagc cactgcacta ttttgcaggc tttactgcgc ggctggggct
    25561 ataacgtcgc gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg gaacaggttt
    25621 ttgatcttgt gctttgcgat gtgcgaatgg cggagatgga cggcattgcc acgctaaaag
    25681 agatcaaagc gttaaatccg gcgattccgg tgctgattat gaccgcatac tccagcgtcg
    25741 agacggcggt agaagcgttg aaaaccgggg cgctggatta tctcatcaag ccgctggatt
    25801 tcgataacct gcaggcgacg ctggaaaagg cgctcgcgca tacgcacagt attgacgctg
    25861 aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg gcgatgcaac
    25921 acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg atccacggcg
    25981 attccggcac cggtaaagag ctggtcgcca gggcgattca cgccagtagc gcacgtagcg
    26041 aaaaaccgct ggtaacgctc aactgtgcgg cactcaacga atccttgctg gaatctgaat
    26101 tgttcggtca cgaaaaaggg gcgttcactg gagccgatag acgccgggaa gggcgctttg
    26161 ttgaggcgga cggcggcacg ctgtttctcg atgaaattgg cgatatctcg ccgatgatgc
    26221 aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt agcaaccaga
    26281 ctatctcggt tgatgtccgg ttgattgcgg cgacccatcg cgatcttgcc gcagaggtga
    26341 atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg attgaagtac
    26401 catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tgtccatttt cttcagcgct
    26461 ttgccgagcg taatcgcaag gcggtaaaag gctttacgcc acaggcgatg gatttgctga
    26521 tccactacga ctggccggga aatattcgtg aactggaaaa cgcggtggaa cgggcggtgg
    26581 tgctgctgac cggggaatat atttccgaac gcgagctgcc gctggcgatt gccggtacgc
    26641 cgatcccact ggggcaaagt caggatattc agccgctggt ggaagtcgaa aaagaagtga
    26701 ttctggcggc actggagaaa acgggcggca acaaaaccga agccgcccgt cagttaggga
    26761 tcacgcgcaa aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt tcgatagcgc
    26821 gccagccgat atctttccgg cagaagcagt cgtcccagtg aatatcggtc attaaggcat
    26881 aagcgcgttt ctgcgcttct gccacggtat gacccagcgc ggtgacgcac agtacgcgcc
    26941 cgccgctggt cactacctgc tcgttatccg ccagtttcgt gcccgcgtgg aacactttgc
    27001 cgcctgccac ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg taatcgcccg
    27061 gatatccacc cgcagccatc accacgccga gagaagcacg ctcatcccac tctgacgttt
    27121 tctcgtccag tttgccttca caggccgcca ggcagagctc aaccagatcg gacttcatgc
    27181 gcagcataat cggctgggtt tctggatcgc caaagcggca gttaaattcg ataaccttcg
    27241 gattgccctg tttgtcgatc atcaggcccg cgtagagaaa accggtgtag gtgttgcctt
    27301 ccgccgccat gcctttcacg gttggccaga tgatacgttc catggtgcgc tgatgaactt
    27361 catcggttac taccggcgcg ggggagtaag cgcccatccc gcccgtgttt ggtccggtat
    27421 ctttatcgcc tacgcgtttg tgatcctggc tggtggccat cggcagcaca tgctcgccgt
    27481 ctaccatcac gataaagctc gcttcttcgc cgtcgaggaa ctcttcgata acgatgcgat
    27541 gacccgcgtc gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct tcggcttctt
    27601 ccagcgtcat cgccacgata acgcctttcc cggctgccag accgtccgct ttaatgacga
    27661 tcggcgcacc tttctcacgc agatacgcca gcgcaggttc tacctcggtg aagttctggt
    27721 attccgctgt agggatctta tggcgggcca ggaaatcttt ggtaaacgct ttcgaacctt
    27781 ccagttgcgc ggcacctgcg gttgggccga agattttcat cccggcggcg cggaaggtat
    27841 cgaccacgcc tttcaccagc ggcgcttccg ggccgacgat ggtcagatca atcttttcgt
    27901 tttgtgcgaa atccaacagc gccgggatat cggtcacgcc gatagcaacg ttttgcagcg
    27961 cgggttccag cgcagtgcct gcattacccg gagcaacaaa aacagtttca accagcggcg
    28021 actgcgccgc tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc actaatactt
    28081 tcatctattg ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca tcgcaatacc
    28141 gtgctcgtcg gcggcggcaa tcacttcgtc atcacggata gaaccgccag gctggattac
    28201 gcaggtcacg cctgcagcgg cggcggcatc aataccgtcg cggaacggga agaacgcgtc
    28261 agaagccatc gaggaacctt tcacttccaa gccttcatcg gcagctttaa taccggcgat
    28321 tttcgcggag tacacgcggc tcatctggcc cgcgccaatc ccgatggtca tattgttttt
    28381 ggcatagacg atagcgttgg atttcacgaa cttcgccact ttccagcaga acagcgcatc
    28441 acgcagttcc tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt ccgcaccgac
    28501 catgcccagg tcacgatcct gaaccagcag accgccgttc acgcgtttga aatcaagacc
    28561 cggaacacgc tcgccccact gaccgcaggt cagaacgcgt acgttctgct tggcggcggt
    28621 gattttcagg gcttcttcgc tggcggaagg cgcaataatc acttcgacaa actgacgaga
    28681 aatgatggcc tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga tgccgccgaa
    28741 tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg aattgccgat
    28801 agccacgccg caagggttgg cgtgcttcac aatcacacat gccggctcgg cgaactcttt
    28861 cacgcactcc agcgccgcat cggtatcggc gatgttgtta taagagaggg ctttaccctg
    28921 aacctgggtt gcggtagcaa cggaggcttc tttcacattc tcttctatat agaaggcagc
    28981 ctgctggtgg ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt tcaggttcag
    29041 cgtgcgtggg aagcgaccgg cggcttcttt gctttcaccg tggtaagccg gaaccatgct
    29101 gccgaagtag ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg ctttgatggc
    29161 gaggtcgaaa cgggttgcaa gcgtcagcga tccttcgttg tcatccatct ctttaataat
    29221 ggcgtcgtag tcgctgctct tcaccacgat tgcgacatct ttatggttct tagcggcgga
    29281 gcgcaccatg gtcgggccgc cgatatcgat gttctcaacc gcatcttcca gcgagcagcc
    29341 ttcacgagca acagtctggg cgaacggata caggttaaca accaccatat cgataggctg
    29401 gatctgatgt tcttccataa tggcatcgtc ctggccgcga cggcccagaa tgccaccatg
    29461 tactttcgga tgcagggtct tcacgcgtcc atccatcatc tccgggaaac cggtgtaatc
    29521 ggaaacttcg gttaccggca gacctttttc tgctaacaga cgggcagtgc cccctgtaga
    29581 cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac cggctttgtc
    29641 agaaacactg agcagagcgc ggcggactgg acgacgttgt tgcatggtaa atcccctgga
    29701 tttgactatt acagagagcg ttagctgaat ttttcgcgaa aaactcagct aacgccccta
    29761 acggggcatc cttatttttc gcccgcattg taacgaaaac gtttgcgcaa cgctcgcgaa
    29821 tttttctctt tcaatggtga tcacaatttt gacagtggtt accgtgggaa aaatacagaa
    29881 attacattga tgattgtgga taactctgtg cgtaaaaagg tataaagcgg ggttttgctg
    29941 gggaatgcag cagtcagtca tttttctgca atttttctat tgcggcctgc ggagaaatcc
    30001 ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac agccggttcg
    30061 gttgaagaga aaaatcctga aattcagggt tgactctgaa agaggaaagc gtaatatacg
    30121 ccacctcgcg acagtgcgct aaa
//
LOCUS       DAZPAC010000028        30120 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZPAC010000028 DAZPAC010000000
VERSION     DAZPAC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30120
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.028"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            37..942
                     /gene="yijE"
                     /locus_tag="ACHUPM_001054"
     CDS             37..942
                     /gene="yijE"
                     /locus_tag="ACHUPM_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR0868902.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(969..1586)
                     /locus_tag="ACHUPM_001055"
     CDS             complement(969..1586)
                     /locus_tag="ACHUPM_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFR0868903.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(1860..2963)
                     /gene="gldA"
                     /locus_tag="ACHUPM_001056"
     CDS             complement(1860..2963)
                     /gene="gldA"
                     /locus_tag="ACHUPM_001056"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFR0868904.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(2974..3636)
                     /gene="fsa"
                     /locus_tag="ACHUPM_001057"
     CDS             complement(2974..3636)
                     /gene="fsa"
                     /locus_tag="ACHUPM_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR0868905.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(3648..6149)
                     /gene="ptsP"
                     /locus_tag="ACHUPM_001058"
     CDS             complement(3648..6149)
                     /gene="ptsP"
                     /locus_tag="ACHUPM_001058"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR0868906.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            6458..7537
                     /locus_tag="ACHUPM_001059"
     CDS             6458..7537
                     /locus_tag="ACHUPM_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR0868907.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTGGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            7552..7872
                     /locus_tag="ACHUPM_001060"
                     /pseudo
     CDS             7552..7872
                     /locus_tag="ACHUPM_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            7923..10219
                     /locus_tag="ACHUPM_001061"
                     /pseudo
     CDS             7923..10219
                     /locus_tag="ACHUPM_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            10185..11063
                     /locus_tag="ACHUPM_001062"
     CDS             10185..11063
                     /locus_tag="ACHUPM_001062"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR0868908.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            11065..11406
                     /locus_tag="ACHUPM_001063"
     CDS             11065..11406
                     /locus_tag="ACHUPM_001063"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR0868909.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(11393..12244)
                     /locus_tag="ACHUPM_001064"
     CDS             complement(11393..12244)
                     /locus_tag="ACHUPM_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0868910.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(12459..14192)
                     /locus_tag="ACHUPM_001065"
     CDS             complement(12459..14192)
                     /locus_tag="ACHUPM_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR0868911.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(14375..17026)
                     /gene="ppc"
                     /locus_tag="ACHUPM_001066"
     CDS             complement(14375..17026)
                     /gene="ppc"
                     /locus_tag="ACHUPM_001066"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR0868912.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(17378..18529)
                     /gene="argE"
                     /locus_tag="ACHUPM_001067"
     CDS             complement(17378..18529)
                     /gene="argE"
                     /locus_tag="ACHUPM_001067"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR0868913.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            18683..19687
                     /gene="argC"
                     /locus_tag="ACHUPM_001068"
     CDS             18683..19687
                     /gene="argC"
                     /locus_tag="ACHUPM_001068"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR0868914.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            19695..20471
                     /gene="argB"
                     /locus_tag="ACHUPM_001069"
     CDS             19695..20471
                     /gene="argB"
                     /locus_tag="ACHUPM_001069"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR0868915.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            20532..21905
                     /gene="argH"
                     /locus_tag="ACHUPM_001070"
     CDS             20532..21905
                     /gene="argH"
                     /locus_tag="ACHUPM_001070"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR0868916.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            22172..23089
                     /gene="oxyR"
                     /locus_tag="ACHUPM_001071"
     CDS             22172..23089
                     /gene="oxyR"
                     /locus_tag="ACHUPM_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR0868917.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPIADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(23072..24472)
                     /gene="sthA"
                     /locus_tag="ACHUPM_001072"
     CDS             complement(23072..24472)
                     /gene="sthA"
                     /locus_tag="ACHUPM_001072"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR0868918.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            24793..25443
                     /gene="fabR"
                     /locus_tag="ACHUPM_001073"
     CDS             24793..25443
                     /gene="fabR"
                     /locus_tag="ACHUPM_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR0868919.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            25443..25802
                     /locus_tag="ACHUPM_001074"
     CDS             25443..25802
                     /locus_tag="ACHUPM_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR0868920.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(25842..26942)
                     /gene="trmA"
                     /locus_tag="ACHUPM_001075"
     CDS             complement(25842..26942)
                     /gene="trmA"
                     /locus_tag="ACHUPM_001075"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR0868921.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      27056..27246
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            27311..29155
                     /gene="btuB"
                     /locus_tag="ACHUPM_001076"
     CDS             27311..29155
                     /gene="btuB"
                     /locus_tag="ACHUPM_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR0868922.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            29100..29957
                     /gene="murI"
                     /locus_tag="ACHUPM_001077"
     CDS             29100..29957
                     /gene="murI"
                     /locus_tag="ACHUPM_001077"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR0868923.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         6863 a         7568 c         8295 g         7394 t
ORIGIN      
        1 atagtgtcca caggaaaact acacactgga tctctcatgt ctgccgcagg aaagagcaac
       61 ccactggcaa tcagtggcct ggttgtgctc acacttatct ggagttatag ctggattttc
      121 atgaagcaag tcaccagcta catcggtgcc ttcgacttta ccgccttacg ctgcattttc
      181 ggcgctctcg ttttattcat cgtcctttta ttacgtggtc gcggaatgcg cccgacaccg
      241 tttaaataca ccttagccat tgccctgtta caaacctgcg ggatggttgg cctggcgcag
      301 tgggcgctgg tcagcggcgg agcggggaag gtggcgatcc taagctatac catgccgttc
      361 tgggtggtga ttttcgccgc gttgtttctc ggtgaacgcc tgcgacgtgg gcaatatttc
      421 gcgatactga ttgccgcttt cggcttattt ttggtgttgc agccgtggca actcgatttc
      481 tcttcgatga aaagtgccat gctggcaatc ctctccggcg tcagttgggg ggcgagcgcg
      541 attgttgcta aacgtctgta tgcccgtcat ccgcgcgtgg atttattgtc gttaacatcc
      601 tggcagatgc tctatgcggc gctggtgatg agtgtggtcg ctttactggt gccgcaacgt
      661 gaaattgact ggcagcccac cgtgttctgg gcgctggcct acagtgcgat tctggcgacg
      721 gcactggcgt ggagcttatg gttgtttgta ttgaaaaact tgcctgccag tattgccagc
      781 ttaagcacac tggccgttcc cgtttgcggc gtactctttt cctggtggct gctcggcgag
      841 aatccggggg ccgttgaagg tagcggtatt gtgctgattg tgatggcact ggcgctggtg
      901 agccgtaaga aaaaagaagc cgttagtgta aaaaggatct gaattttttc ttcatgtggg
      961 gcgatcgctt atttaacaaa ataacgataa tgccccacca tgcgccagct aaacagcaca
     1021 tcttcttcct gcgcacctgc gccaatgtta tgtatcacca gcggcgtacc gtcgcgggcg
     1081 aagccatctg aaaccacccc aatatgtgcc agcccgttat ctaatcgcca ggagacaata
     1141 tcgcccgctt gatagtcact ggggttcttg ctgatggggc gtgttttatc gtggtgggta
     1201 aaccaggttt ccagattagg cacccgacgg tgatcgatgt tgctgtccgg gcgctttaac
     1261 tgccattttt gcgggtactc ggcaaaattc ttcgccatat cttcgtgcac cagtttctgc
     1321 aaatcgacct tctggctgcg caatgcacgg atcaccacat cggaacatac cccgcgtttt
     1381 tgcgggacat caccgccagg ataggtaagc tgcatatacg ccggatcgta aaatagcgtg
     1441 ctgccgattt gctgtctggc accgtctgcg atggcaaggt tggtattggc ctggatttgt
     1501 accacggttg gtggaacggc gggagatttt aaggagtggc tggtaaatgc cgttagcagg
     1561 ctgagcagcg ccagtgaagc tttcatctgg ttcgtccgtg aaaataagag tcacagattc
     1621 gaccttccgg gataacgatt gttctgtaga gaaaatgtcc agaagcggat aaaaaactcc
     1681 tgaacaacgt gggagtttca gattactggc acagagtgcc ggatgcggcg tgaacgcctt
     1741 atctgtccta aaaaaaaaga aaattcaata tattgcagag attgcgtagg cctgataagc
     1801 gtagcgcatc aggcaatttt gcgttcaaac tcccggacaa gccgggagtt tggagtaggt
     1861 tattcccact cttgcaggaa acgctgaccg tactggtcgg ctaccagcag agcggcgtaa
     1921 acctgatctg gcgtcgcgcc gccaggcatg ttgtggatgg tttcacattc tgcacatgcc
     1981 gcttcagcca caattcgcat tttcgccggg acatcttctt taatatccag ttgagcgaga
     2041 gttattggca aacctaccgc atggctaagt gcagctacgg tttcgatttc ctcaaccggt
     2101 gcgttttcca gaaccagctg cgtcagcgta ccaaatgcca ctttttcacc gtgataatag
     2161 tgatgcgcgt ccgggatagc ggtcaggccg ttatgcactg cgtgcgccgc agccagacca
     2221 ccactttcaa aaccaacacc gctcaaatag gtgttcgctt caatcacgcg ctccagcgcc
     2281 ggagtcacta catgctgttc ggcagcaagc atcgcttttt cgccttcttc cagcagggtg
     2341 ttgtagcaca gttcagccag tgccagcgca gcctgggtgc acttgccgcc cgccatggtg
     2401 gtcgcgccgc tacgagagca ggcacgcgct tcaaaccagg ttgccagcgc atcgccgata
     2461 cccgccgcta acagacgtgc aggtgcgcca gcgacgattt tggtgtcgac aatgaccata
     2521 ttcgggttat ttggcaacag cagatagcgg tcaaactcac cctcatcggt gtagataaca
     2581 gacaatgcgc tgcacggtgc atcggtagag gcgatagtcg gtgcgatcgc taccggaaca
     2641 cccatgaaat gtgccagtgc tttggctgta tcgagggttt ttccgccacc gataccgaga
     2701 attgcgccac actgcgcagt ctccgcgatg ccacgcagac ggtcgatctc attttgcgaa
     2761 catccaccgc caaacggcgc aatttctact accagtccag catctttaaa gcttttctcg
     2821 acagtggatt gagcaaaacc taaaacaaat ttgtcaccca ccactaacca gcgttctgcc
     2881 agcggcttca ggtattcgcc cagacgatta atcacatcag cgccctggat gtatttaccc
     2941 ggtgattgaa taatgcggtc cataattgct cctttagaga tgagtagtgc caaatgcggc
     3001 attccagtcg tgttcgaact tctctatagc tgactctacc gcaggggtgt tgagcatttg
     3061 ttgcgctaca tctaagggca gggtgatgga ttcacatcct gccagtaaac agtccagcgc
     3121 ctgacgcggc gttttaaagc tggctgccag caccatgctt tctggcgcgt gcatttctaa
     3181 cagcgcttgc agctcctgaa ccgtacgaat gccgtctccg ccctgggcat ctacgcggtt
     3241 aacatacgga gcaacgtatt ttgcccctgc cagcgcggcc agtaaccctt gtgcagcact
     3301 gtacactgcg gtgccaagcg tagtaatacc ctcttttttc agtattttaa ttgctgccag
     3361 accttcggaa gtcaccggga ttttcaccac aatacccgga atagcgtcgc gcaggcgctt
     3421 cgcttcttcc accatcccct gcgcgtcgcg gctcatggtc tgagcaaaca gaatgccctc
     3481 atcaccaatc gctttttgca gacgcggcag cacttcccat atggactcct tgctggcagc
     3541 gataatgctc gggttagttg tcaccccggc aatggggaat atgcgtgcca gacgttcgac
     3601 ttctgcgacg ttagcggtgt ccagatacag ttccatgatg tttcctttta cagttccagt
     3661 tcatgttgca gcaggctggc gatagcgtcg gcagaggcgg cgttaaccag cgcgttacgg
     3721 aattcttcgt gcatgatgcg gcgagcgagg cgcgagaaaa tgcgcatgtg ctgatcgccc
     3781 gcagcgtgtt tgttcagggt taacatgatg atgaattgcg cttcatcatc accccagcgc
     3841 accggcgctt gcagacgcgc aacgctgatg gtggattgct caatgtgttc tgatttgctg
     3901 tgtggaatgg caaaactaaa gcccagaccg gtagagaaaa cggcctcgcg cgcccacaag
     3961 tcggcttcca gtttacgcgg atagcggcag cgccctgcca gcagcaggtt atcggtcatg
     4021 cctttgagca cttcttcttt gctgcgccag tcgctttcca gtgtgatgca ctcggcggtg
     4081 accagcggtg cgtcttgttg ggtcatgcgg aattgcgcca gcaggtgctc cacttccagc
     4141 gaagtacggc aggccattgc ctggttgagc aacttgcggc actcacggct atcaagttgc
     4201 gccatccgcg ctttcgccgc cggaattgat ggtgcgctca tgctgagttc atccagccct
     4261 aagccgacca gcaacggcag cacggaacct ttcgctccca gctcaccgca cagaccaatc
     4321 catttaccct ggcgatgcac cgcctgcacg gcgtaatcga gcgcccgcaa gaatgccgga
     4381 ttcaggctgt tgtagtgacg agtaacctta gcgttatcgc gatccactgc cagcagatat
     4441 tgcgtcaggt cattactacc aatactaaag aaatcaatct cttcgcagca ttgatcgatg
     4501 atgaacatca ccgacggcac ttccagcata atgccgagct ggatcttctc atcaaacgga
     4561 atgtgttcgt tacgtagttg ctgtttggct tctgccagtt tttctttcac ccataagatc
     4621 tcttccattg aggagatcat cgggatcatg attttcaggc tgccgtgagc ggaggcgcgc
     4681 aggatcgagc gtagttgtgt ggtaaataac gacgcgtatt cttcataaat acgcacggcg
     4741 cgataaccga ggaacgggtt tgcctctgcg ggaatgttta gataatcaac gggtttgtcg
     4801 ccgccaatgt ccatagtgcg cacaataatg ctgcgtccgt tggcagactc cagcgcctga
     4861 caaaaaatgt tgtacagctc gctttcgccc ggtgcactgg tgcgatccat atagagcatt
     4921 tcagtgcgga acaaaccaac gccttccgca ccattgccga atgcggcctg cgcttccacg
     4981 gagtgagcga tgttagcggc aatttcaatg cggataccgt cagcggtacg ggcttgttgg
     5041 gtcagccaga cacgctgttg ctcacgcagg gcgtcctgta cgcgggcttc ttgctgataa
     5101 taacgagcta cggcttctcc tggctcaacc acaatcgccc cggcgttgcc gtcgatataa
     5161 atcgtttgat gctgccacgg agtgagggca tcaatatcca caccaaccag cgtcggaatg
     5221 ttgaacgaac gggcaaggat caccgtatgt gaggtggtgc cgccactttt cagcaacaga
     5281 cctttgaggt gatttttatc cagttcgagg aactggctgg gggtcagttc atcagccata
     5341 caaatggcgg actgcgtcag tttgcccggt gccgggaagc gttgctcacc gtagatttgc
     5401 tggagtaact ggaagcagac gtcgcgtacg tccagggcac gttcttgcag gtagctgctg
     5461 ctggaacggg cgaactcttc gcaaaagtga ttcgcactgg cgacaatcgc ttcggcgcag
     5521 cttaatccct cgctgacgcc tgccagtaag tgctgacgta aagatgtatc gccagccagg
     5581 gatcggtgag cttccagtat cgcgctggta gcaccgtcgc tatccagcag acgaaactca
     5641 atgtttttca gtaccagcgt caggccgttt tccagtgtgg attgctcggc gtcaacgtct
     5701 ttggctgcgg gaagattact cagcgcattg agatctaaag aagagatcgg cgtcagaatg
     5761 ccgcctgcac taccgctgca cacggtgcgg gcgcggataa tttgcggatt cagattggtc
     5821 agtgaaaccg gcagtggttc cagttcgtca gatttaactt ccgccagcgg cgcatcgcag
     5881 tgggggaatt catcgcgcag ccattggctt aaacgctggt gcgcttcctg ttcgtcggcc
     5941 ccggaaatca gtaactggca gttatcgccc gccagcgtat cggtgccaat cagcgcaagg
     6001 gcgcttttgg cgttgccctt gcggtcagtg tgcaggttat gccactcaat ttgtgatgaa
     6061 aaagtattac acagcgtttc aacgtggctt gccggacgcg catgtacgcc gttaggtagc
     6121 tcacaaataa attccacaat cagggccatt gcctctctcc cggtacggtt ttctgtttac
     6181 aggataagag cgcacggcaa cggcctgcca tgtgacaaat ctgccaaaag ctggacaaat
     6241 gtaatgtaac cgtcaatttg cgacgcgtct cacaagacgc cgttttgcgg catgcttccg
     6301 gtttatcgca agttatgagg cggatcgcat ttttgtactg atattacaaa agtccagtaa
     6361 atggcctttt tatccactgt ttgctccgcc tgcgattgcc tattgtttcc tccatcaaaa
     6421 atatggacat gggctttcgc aagcccggga gcagcttatg aatgagttgg tgcagatcct
     6481 gaaaaatacc cgtcagcatt taatgacggg cgtttcacac atgattccct tcgtggtatc
     6541 gggcggtatt ttgctggcgg tttccgtcat gttgtatggc aaaggcgcag tgccggatgc
     6601 cgtagccgat ccaaatctga aaaaactgtt tgatatcggc gttgcgggct tgacgctgat
     6661 ggtgcctttc ctcgccgctt acattggtta ttccattgca gaacgttctg cgctggctcc
     6721 atgcgctatc ggtacctggg ttggtaacag ctttggtgcg ggcttctttg gtgcactgat
     6781 cgccgggatt atcggcggca tcgtggtgca ttacctgaag aaaattccgg tgcataaagt
     6841 tctgcgctcg gtgatgccta tcttcattat tcctatcgtc ggcacactga ttaccgcagg
     6901 catcatgatg tggggcttgg gcgagcctgt aggggcgttg accaacagcc tgactcagtg
     6961 gcttcagggg atgcagcagg gcagcattgt tatgctggcg gtgatcatgg gtctgatgct
     7021 ggcgttcgat atgggcggtc cggttaacaa agtggcctat gccttcatgc tgatttgcgt
     7081 tgctcagggt gtttataccg tggtggccat cgctgccgtg ggtatttgta tcccgccgct
     7141 ggggatgggg ctggcgacgc tgattggtcg taaaaatttc tccgcagaag agcgcgaaac
     7201 cggcaaagcg gcactggtga tgggctgtgt tggcgttact ggaggggcga ttcctttcgc
     7261 cgctgccgat ccgctgcgtg tcattccttc catcatggtc ggttcagttt gtggtgcagt
     7321 aactgcggcg ctggtcggtg cgcagtgcta tgcaggctgg ggtggtctga ttgtgctgcc
     7381 ggtggttgaa ggcaagctgg gttatatcgc agcagtggct gtcggagcag tggtgacggc
     7441 tgtttgtgtg aacgtgctga aaagtctggc gcgtaaaaat gggtcttcga ctgatgaaaa
     7501 agaagacgac ctggatttgg attttgaaat taattaattg aggaacgagc catgacgaaa
     7561 attattgcag taaccgcatg tccttcaggt gttgcccata cctatatggc agcagaggcg
     7621 ctggaaagtg ccgcgaaagc gaaaggctgg gaagtgaaag tagaaaccta ggggtcaatt
     7681 ggtctggaaa atgaactgac tgcggaagat gtggcgagcg ctgatatggt gattctgacc
     7741 aaagatatcg gcatcaagtt tgaagagcgt tttgctggca agaccatcgt gcgcgtcaac
     7801 atcagcgatg cagtaaaacg tgctgacgcc attatgagca aaattgaagc tcacctggcg
     7861 caaaccgctt aagttcttct cccgctcgca agggcgggtt cgctttccca caggagttcc
     7921 tcatgacgaa tcgtatctct cgcctcaaaa ctgcactgtt tgccaatacc cgtgaaatct
     7981 cgctggagcg ggcgctgctt tataccgcca gccatcggca aaccgaaggc gaaccggtga
     8041 tattgcgccg ggcgaaagca acagcgtata tccttgaaca tgttgaaatt tcgattcgtg
     8101 atgaagaact gattgccggt aaccgcaccg taaaaccgcg cgccgggatt atgtcgccgg
     8161 aaatggaccc ttactggctg ctgaaagagc tggatcaatt cccgacgcgt ccgcaggacc
     8221 gctttgctat cagcgaagaa gataaacgta tctaccgtga agagttgttc ccgtactggg
     8281 aaaaacgttc gatgaaagat ttcatcaacg ggcagatgac agatgaagta aaagccgcga
     8341 ccaacacgca gattttcagc atcaaccaga cggataaagg ccaggggcac attattattg
     8401 attacccacg cctgttgaat cacggtctgg gtgagctggt ggcacagatg cagcaacatt
     8461 gtcagcaaca gccggagaat cacttttatc aggccgcgtt gttactgctg gaagcctcgc
     8521 agaaacacat tttgcgttac gccgaactgg cggaaacgat ggcggcaaac tgcacagatg
     8581 cacagcgtcg cgaagaactg ctgactattg cggagatctc tcgccataac gccgaacata
     8641 agccgcagac gttctggcag gcgtgccagt tattctggta catgaacatc attctgcaat
     8701 acgaatccaa cgccagttcg ctgtctttag ggcgcttcga ccagtatatg ttgccgttct
     8761 atcaggcatc tttaacccag ggcgaagatc cggcgttcct gaaagaactg ctcgaatctt
     8821 tatgggtgaa atgcaacgac atcgtgctgt tgcgctccac cagcagcgcg cgttatttcg
     8881 caggtttccc gaccggctat accgcactgc tcggcgggtt aaccgagaac ggacgtagcg
     8941 cggtgaacgt gctttcgttc ctttgccttg acgcctatca aagcgtgcaa ttaccgcaac
     9001 cgaacctcgg cgtgcgcact aacgccttga tcgacacgcc gttcctgatg aaaaccgccg
     9061 aaaccattcg cctcggcacc ggtattccgc aaatctttaa cgatgaagtg gtggtaccag
     9121 cgttcctcaa ccgtggcgtt tcgctggaag atgcgcgcga ctattccgta gtgggctgtg
     9181 tggaattatc tattcccggc agaacctacg gcttgcatga catcgcgatg tttaatctgc
     9241 tgaaagtgat ggaaatctgc ctgcatgaaa atgaaggcaa tgctgcgctg acttatgaag
     9301 gtttactgga gcagatccgc gccaagatca gccactacat caccctgatg gttgagggca
     9361 gcaatatttg tgatatcggc catcgcgact gggcacctgt accgctgctc tcgtctttta
     9421 tcagcgattg tctggaaaaa ggtcgcgata ttaccgatgg cggcgcgcgt tataacttct
     9481 ccggcgtaca ggggatcggt atcgccaacc tgagcgattc tctccatgcg ttgaaaggga
     9541 tggtttttga gcaacagcgt ttaagttttg acgaattgct gtcggtatta aaagccaact
     9601 ttgcaacgcc agaaggcgaa aaagtccgtg ctcgcttaat taaccgcttc gagaaatacg
     9661 gtaacgatat cgacgaggtg gataacatca gcgccgaact gttacgccac tactgcaaag
     9721 aagtggaaaa ataccagaac ccgcgcgcgg ttacttcacg ccgggatcgt ataccgtttc
     9781 tgctcacgtc ccgttgggat cggtggttgg cgcgacgcca gacggtcgtt ttgccgggga
     9841 acaactggca gacggcggct tgtcacctat gctgggccag gacgcacaag ggccaacagc
     9901 ggtactgaag tcagtcagta agctcgataa tacgctgctg tctaacggta cgttgctgaa
     9961 cgtgaaattc actccggcga ccctggaagg tgaagcggga ttacgcaaac tggccgactt
    10021 cttacgggcg tttacccagc ttaagttgca gcatattcag tttaacgtgg tgaacgccga
    10081 cacgttgcgg gaagcgcaac agcgcccaca agattatgcc gggctggtgg tgcgcgttgc
    10141 cggatacagc gccttctttg tcgaactgtc gaaggagatc caggatgaca tcatccgccg
    10201 gacagcgcat cagctgtaac gttgtggaaa cgcgccgcga tgatgtggcg cgcattttca
    10261 acattcagcg ttattcactg aatgacggcg agggcattcg tacggtggtc ttttttaaag
    10321 gctgtccgca tctttgcccg tggtgtgcta atccggagtc gatctccggc aaaatccaga
    10381 cggtacgcag agaggcgaaa tgtctgcact gtgcgaaatg tttgcgtgat gcggatgaat
    10441 gcccctccgg ggcgtttgaa cggattggtc gcgatatcag ccttgacgct ctggaacggg
    10501 aagtgatgaa agatgacatt ttctttcgca cgtccggcgg cggcgtcacg ctttctggcg
    10561 gcgaagtgtt aatgcaggcg gagtttgcta cccgtttttt acagcgactg cggctgtggg
    10621 gtgtgtcatg cgccattgaa actgccggag acgcgccagc aagcaagctg ttaccgctgg
    10681 cgaaattgtg cgatgaagtg ctgttcgatt taaaaattat ggacgcgact caggcgcggg
    10741 atgtggtgaa gatgaacctg ccacgcgtgc tggagaatct gcgtttgctg gtgagtgagg
    10801 gtgtcaacgt gatcccgcgt ttaccgttga tccctggttt cacgctcagc agggagaata
    10861 tgcagcaggc gctggatgta ctgatcccgc tgaaaatcag gcagatccat ctgttaccgt
    10921 ttcatcagta cggcgaaccg aaataccgcc tgctggggaa aacatggtcg atgaaagagg
    10981 tgcctgcgcc gtcgtcagcc gatgtgacaa cgatgcgcga aatggcagaa cgggccggat
    11041 ttcaggttac cgtgggaggt taaaatggca tacctggtgg cagtaaccgc ctgcgtaagc
    11101 ggcgtggcgc atacttatat ggcggcggaa cggctggaaa agttgtgcca gttagagaag
    11161 tggggcgtca gcattgaaac tcagggcgcg ctgggaacgg agaatcgttt agcggacgag
    11221 gatatccgtc gggcggatgt tgctctgttg attacggata tcgagcttgc cggtgccgag
    11281 cgatttgaac attgccgcta tgtgcaatgc agcatctacg cattcctgcg tgagccgcag
    11341 cgggtaatga gcgcggtgcg caaagtgctt tctgcgccgc agcaaaccca tcttattctg
    11401 gagtagtcgg tttttctgtc agttggctgt ggtactgccg gcgatattcc gacggcgagc
    11461 gttcggtgtt tttacgaaac agacggcaga agtagttgct gtcgacaaag ccgcaggcgt
    11521 gcgccacttc ttttaccttc aggtcatagc ctttcaataa cgtcttagcg tgctccagtc
    11581 gcgtgtgatt caggtattcg ttaaaaccaa tggcccccgt tttttgaaac aggtgcgaga
    11641 gatagtttgg tgaaatatag aatgcctgtg cgacagattc gcgggtaagc gcggaggcat
    11701 agcgttcgtc gatataatcg cgaatagctt caaatagtgc ctggctgcgt gaggcggtct
    11761 ggatttggct gccgagcaaa tcgcggcagt ggctaagcag gctggctacg ataagccttg
    11821 ccgtttgctg ctcctgcggc tgcatttgca tttcattgag cgtttgtagc agaaaagaac
    11881 ctatacgtgg gccgcgtcgg gcgacgtgtt gcttcgccag attttgatat tgtttgccat
    11941 cccattgcac gacgctgaag ccgagttgct gtttgccaaa cagcacgcta aaggtggtag
    12001 cgggtgtttt ccattgtggg aaattccagc caccagcggc aacgtacagc acatcgccgg
    12061 gaactaacgt tgctccagcg ccggtatcaa caaactcacc ttccagcaca atttccagcc
    12121 gtggaaaatc gacctgatac gcaagatcgg gcaccgggcc gttactgctg gcaaagtaaa
    12181 tctggcgcag ggacagcggg ccgttgatca ggcgggagag cagatagctg acgccgtgat
    12241 acatgttatt cccttaggta tgtagacggg aaaacagtaa ctggcattat taaaaaaggc
    12301 cacctgaaaa gtgtggcctg aggggagttc gatgtaaaca ttgcggcagt tgtattgccg
    12361 gatgtggcgt aaacgcctta tccagcctac cgccgcgatc tgtaggccgg ataagacgcg
    12421 tcaagcgtcg catccggcaa atagcgcctg gctgataatt actgattacc tacctggtcg
    12481 ccatacggca gagtgtcgta atcgatcagc gcgtttttct tgtacgggtt accaatccag
    12541 cgggtagttt ctttgaactg cggattcacc actgaacggg ttggatcgta accgtcgtaa
    12601 gataagcccg ccaaatctga ccaggtgtgg atcagttccg ccaggctgta tttacgatcg
    12661 acatcctgtg agaaatcacg gggatgagtc gcttgccatt tttccgacgt ccacagcagg
    12721 aacggaatgg tgtacatgtg gcgcgtcggg ttgtcttcgt tacgcccctg agttttgtgc
    12781 ggcggcgtgt cgtaaacctc ttcaccgtgg tcagagaagt aaaccaggaa tccgttcggg
    12841 tctgccgctt taaagtcttt aatcaggctg gcaaccacat gatcgttata caggttagcg
    12901 ttgtcataat cgttatatga ttccagctct tctgcgctta atcctggcgg aacatgatcg
    12961 gtattgccat caaacttgcc ctgattttcc gggtagcggt atttgtattt gatatgcgta
    13021 cccagcagat gaacgatgat cagtttcttc ggcgcagggt cattcagcac atcctggaac
    13081 ggcttcagca cgttggtgtc gtattcacgc gcactctgcg tacgttgctg gttcatgtag
    13141 tactgcttgt cggtctggcg cgaaaatacc gtcagcatgg tattgcgggc ggtcatcgtc
    13201 tgctggttgg tgatccagaa ggttttataa cccgcctgtt tcatcatgtt catcagcgac
    13261 ggctgcgtca gatacagatc cgggttcttt tcattggcaa aggtcagcgc ctgttgcagg
    13321 atttcaatgg tgtacggacg agacgtaact acgttattaa acacggtcag attcggatcg
    13381 gttttatgca gcgcatccag ctccggcgtg gtttcacgcg gataaccgta cagactcatg
    13441 cgtccgcgct gggtcgactc gccaatcacc agcactaaag tgcgcggttc gttacccgat
    13501 tcatctttga aattagccag tggcggcaag gcattatttt cattcagtaa ctttgttagc
    13561 gagtttagtt gctgacgata ctgataatag ccggtcagga attgccacgg tgcggcaggc
    13621 tccatacgcg aggccaggtt atccaacgtt ttctcaaacg gcttgttttt gataaacgta
    13681 ttcatggcga tcggatgcag aatcaagcca taaagcaggg caaaagagac aacataacgc
    13741 cacggctttg gaatatagac cgggcgcagg cgtgtccaca gcagaactgc caccgccgta
    13801 taggccagcg cgataagcac aattttcagg ctgaaatact ggcttaaata ctcgctggct
    13861 tcgttggtgt tggtttcgaa catcacaaac agaacgctct gcgagaactc ctgcccgtag
    13921 atgacgtagt agcatagcgc cgccagagag gccgcccata gcaccacgcc gattactgcg
    13981 gcaataattt taatccgctt cggaaagagg aataccggga tcaaccacag cgagctgaat
    14041 aacagcgagt cgcgaatgcc gttggtgcca ctataaccac tgatgtaaat aatggcctgt
    14101 agcagagtag agaaaaacca aaagtagagc agtgcccaac ccagggcttt ccagctaaaa
    14161 aggggtttag cctggacttc tgtggaatgc atagtaagaa cctgtcttga aaaaaatatc
    14221 gccgaatgta acgacaattc cttaaggata tctgaaggta tattcagaat ttgaataaaa
    14281 tgcagacaga aatatattga aaacgagggt gttagaacag aagtatttca gaaaaccctc
    14341 gcgcaaaagc acgagggttt gcagaagagg aagattagcc ggtattacgc atacctgccg
    14401 caatcccggc aatagtgacc attaacgctt gttcgacgcg aggatccggt tcctggcctt
    14461 ctttttctgc ctggcgggag cggtgcagca actcggcctg caatacgttc agcgggtcgg
    14521 tgtaaatatt ccgtagctga atagactctg caatccacgg cagatcggcc atcagatggg
    14581 aatcgttggc aatcgccagc accactttga tgtcttcttc ttgcaggttg cgtaactctt
    14641 tacctaacgg ccacagtgct ttgtctacca gacgttgatc atagtattcc gccaaccaca
    14701 ggtctgcttt ggcgaagacc atctccagca tgccgagacg cgtcgagaag aatggccaat
    14761 cgcggcacat ggcttccagc tcgctctgtt tgccatcttc gaccactttt tgcagcgccg
    14821 tacctgcacc cagccaggcg gggagcatca ggcggttttg cgtccaggca aaaatccacg
    14881 gtatggcgcg cagcgactcg acgccgccgg ttgggcgacg tttcgccgga cgtgaaccca
    14941 acggcagttt gcccaattct tgttccggcg tagcggagcg gaagtaaggc acaaaatctt
    15001 tgttttcacg tacgtagcca cggtagagat cgcaggagat gactgacagt tcatccataa
    15061 tgcgacgcca gctctctttc ggctcaggtg gtggcagcag gttggcttcc agaatcgccc
    15121 cggtgtaaag cgacaggctg ctgacggtga tttctggcag accatattta aagcggatca
    15181 tctcgccctg ttcagtcacg cgcaggccac ctttcaggct tcctggcggt tgtgacagca
    15241 gcgccgcatg ggcaggtgcg ccgccgcgac caatggaacc gccgcgaccg tggaacaacg
    15301 tcagctcaat acccgctttt tcgcaggttt tgattaatgc atcctgtgcc tgatattgcg
    15361 cccaggaagc tgccatcacg cccgcatctt ttgctgagtc ggaatagcca atcatcacca
    15421 tctgtttgcc ctgaatcagg ccgcgatacc agtcgatatt gagcagctgg gtcatgacat
    15481 cgttggcgtt gttcaggtca tcgagggttt caaacagcgg agcaaccggc atcgcaaacc
    15541 cgatacccgc ttctttcagc agcaggtgga cagccagtac gtcggacggc gttttcgcca
    15601 tcgagatcac gtaggcggca atggagcctt gcggcgcttc ggcaatcacc tggcaggtat
    15661 cgagcacttc gcgcgtttcg gcgcttggtt gccagttgcg cggcagaagc ggacgtttgg
    15721 agttcagttc gcggatcagg aacgcctgtt tgtcggcctc tgaccagctt tcgtagtcgc
    15781 cgataccgag gtagcgggtc agctcgccca gcgcttcggt atggcgggtg ctctcctgac
    15841 ggatatcaat acggaccagc ggtacgccga aacatttcac gcggcgcagg gtgtcgagca
    15901 gatcgccgtt ggcgataata cccatgccac acgcctgaag tgactggtag caagcgtaga
    15961 gcggttccca cagttcttcg ttttgtgtca gcagcccttc tggttttggc agttcttcgc
    16021 ctttcaggcg cgcttccagc catgcctgtg tcgccatcag gcgagaacgc aggtttttca
    16081 tcagatagcg atacggttct gcggcacctt cttcgccaac cagcgccagc agttcagggg
    16141 tcgcttcaac catcgacagt tcagaaacca gaacctgaat atctttcagg aacagatcgg
    16201 tggctttcca gcgactgagt agcaggacgt ggcgggtgat atcggcagtg acgttcgggt
    16261 tgccgtcgcg gtcaccgccc atccacgaag taaaacggac cggaacaaat tcgacgggca
    16321 gtttgtagcc gaggttctct tccagttgtt cgttcagttc gcgcaagtaa ttcggtacgc
    16381 cttgccacag gctgttttct actacggcaa agccccattt ggcttcatct accgggcttg
    16441 gacgcagctt acggatttca tcagtatgcc atgactgggc gattaactgg cgcaggcgac
    16501 gcatcagctg gttgtgttcg tagtcagcga tatctttgtt atcgagctgt tttaagcagg
    16561 cgttcacttc caccattttg tggatcagtg tacgacgggt aatttcggtt gggtgagccg
    16621 tgaggaccag ttccagcgac agcgattcca ctgctttttt gatggtgtct tcgctcagtt
    16681 ccggctggtt tttcagtttg cgcagggtgc gggcgatcac ttccgggttg ctggcagctt
    16741 cgcctttcgg cgaaatgctg tggtattgct cggcggtgtt ggccaggttc aggaactgac
    16801 taaacgcacg cgcaacgggc agcagctcgt cgttcgacaa attttgtaag gtggtgagca
    16861 actcctggcg gttagcatca ttgccagcgc gtgaagattt cgacaactta cggatagttt
    16921 ctacgcgttc aagaatgtgt tctcccaacg catccttgat ggtttctccc agcactttgc
    16981 cgagcatact gacattacta cgcaatgcgg aatattgttc gttcatatta ccccagacac
    17041 cccatcttat cgtttgatag ccctgtatcc ttcacgtcgc attggcgcga atatgctcgg
    17101 gctttgcttt tcgtcgtctt ttataaagcc acgtaaaagc ggtgacgtca aatgctgcga
    17161 aatcgcttca gcaaacgaat aaatagcagg aatttacgcc attaaattca cgacgcttta
    17221 aataagcgta acttatggaa atgttaaaaa atcgccatac ataacaccaa aggtgtaggt
    17281 cggataagat gcgcaagcat cgcatccgac attattgcgg caccggagtt tggcaacggt
    17341 gccggatgcg gcgcgagcgc cttatccggc ctacgtttta atgccagcaa aaatggtgaa
    17401 ttacctggat aatcaactca cgggtgggtt taataaaccg cgtctccaga tattcatcag
    17461 gctgatgagc ttgattgatg gaacccggac cgagcaccag cgtcgggcat aacgtttgaa
    17521 taaacggcgc ttcggtacag taattcacca cttcggtttt tgctccgagc aatttctcaa
    17581 ccacttcaac cagttgatga ttcggtgggc attcatagcc agggatcggc ggatgcagct
    17641 cgtcgaccgt cagacgaccc ggccagcgtt cgctcaccgg agccaatgca tcgttgagca
    17701 aaccattaag ttcattgagt gtcatgctag gcagcggacg aatatccata tgcaactcac
    17761 agcaagcgca aatacggtta gaagcgtcgc caccgtgaat atgcccgagg ttgagcgtag
    17821 ggtatggcac ggtaaatgct tcgtagtgat aacgttcttt caggttatcg cgcaattgca
    17881 aaatatgccc gatggcgtcg tgcattagtt cgatagcgtt aactccgcgt gctggatcgc
    17941 tggagtgccc cgactggccc tgaatacgga tggcgttaga gatatgacct ttatgtgcgc
    18001 gtaccggttg tagtgacgtc ggttcgccaa tgatggcgca atccgggcgc agggcggtag
    18061 tttcggcaaa ataacgcgct ccggccatac tggtttcttc atcagcagtc gccagaatgt
    18121 agagcggttt tttcagtttc gtgacgtcga catcgcgtag cgtatcaagg ataaacgcaa
    18181 aaaagccttt catgtcggcg gtgcctaagc cgtaaagctt gccgtcatgc tccgtcagtg
    18241 taaacggatc gcgcgtccag cgaccgtcat caaatggcac cgtatcggta tgccccgcca
    18301 gcaacaagcc gccagccccc tgtccgatac ttgccagcat attgaatttg ttgcgagttc
    18361 ctggcacagg ctgcacttcc acattgaagc ctaaatcttt aaaccagtcc gccagcagag
    18421 tgattaaatc tgcattgctt tgatcgagtg cctcttccgt ggcgcttatt gaaggtgtgg
    18481 caatcagagc acggtaaatc tcgataaatg gcggtaattt gtttttcatt gttgacacac
    18541 ctctggtcat gatagtatca atattcatgc agtatttatg aataaaaata cactaacgtt
    18601 gagcgtaata aaacccacca gccgtaaggt gaatgtttta cgtttaacct ggcaaccaga
    18661 cataagaagg tgaatagccc cgatgttgaa tacgctgatt gtgggtgcca gcggctacgc
    18721 tggcgcagag ctagtgacct atgtaaatcg ccatccgcat atgaacataa ccgctttgac
    18781 tgtttcagcg caaagcaatg atgcgggaaa gttaatctcc gatttgcatc cgcagctaaa
    18841 aggcatcgtt gagctgccgt tgcagccgat gtcggatatc agcgagttta gcccaggggt
    18901 ggacgtagtg tttctcgcca ccgcccatga agttagccac gatttagcgc cgcagtttct
    18961 tgaagcgggc tgcgtggtgt tcgacctttc cggtgcgttt cgtgttaacg acgccacctt
    19021 ctatgaaaaa tattacggct ttacccatca atacccggaa ctgctggaac aggcagccta
    19081 cggtctggcg gagtggtgcg gtaataaatt aaaagaagcg aatttgattg cggtgccagg
    19141 ctgttatccg acggcggcac agctggcgct gaaaccgttg attgatgccg atcttcttga
    19201 cctcaatcag tggccggtga tcaacgccac cagcggcgtg agcggtgcag gacgtaaggc
    19261 agcgatttca aatagttttt gtgaagttag cctgcaaccg tatggcgtct ttactcatcg
    19321 ccatcaacca gagatcgcca cacacctcgg tgctgacgtt atcttcaccc cacatctggg
    19381 caatttcccg cgcggcattc tcgaaaccat tacctgccgc ctgaaatcgg gtgtgaccca
    19441 ggcgcaagtc gcgcaagtgt tacagcaggc gtatgcccat aaaccgctgg tgcggctgta
    19501 tgacaaaggc gttccggcgc tgaaaaatgt cgttgggctg ccattttgcg atatcgggtt
    19561 tgccgttcag ggcgagcatc tgattattgt ggcgaccgaa gacaacttac tgaaaggcgc
    19621 ggcggcacaa gcggtacagt gcgccaatat tcgtttcggc tatgcggaaa cgcagtctct
    19681 tatttaaggg tgcaatgatg aatccattaa ttatcaaact gggcggcgta ctgctggata
    19741 gtgaagaggc gctggaacgt ctgtttagca cactggtgaa ttatcgtgag tcacatcagc
    19801 gtccgctggt gattgtgcac ggcggcggtt gcgtggtgga tgagctgatg aaagggctga
    19861 atctgccggt gaaaaagaaa aacggcctgc gggtgacgcc tgctgatcag atagacatta
    19921 tcaccggagc actggcggga acggcaaata aaaccctgtt ggcatgggcg aagaaacatc
    19981 agattgcggc cgtaggtttg tttctcggtg acggcgacag cgtcaaagtg acccagcttg
    20041 atgaagagtt aggtcatgtt ggactggcgc agccaggttc gcctaagctt atcaactcct
    20101 tgctggagaa cggttatctg ccggtggtca gctccattgg cgtaacagac gaagggcaac
    20161 tgatgaacgt caatgccgac caggcggcaa cggcgctggc ggcaacgctg ggcgcggatc
    20221 tgattttgct ctccgacgtc agcggcattc tcgacggcaa agggcaacgc attgccgaaa
    20281 tgaccgccgc gaaagcagaa caactgattg agcagggcat tattactgac ggcatgatag
    20341 tgaaagtgaa cgcggcgctg gatgcggccc gcacgctggg ccgtccggta gatatcgcct
    20401 cctggcgtca tgcggagcag cttccggcac tgtttaacgg tatgccgatg ggtacgcgga
    20461 ttttagctta agttttgttg gccggaggcg cagctttccg gcattgaatt tcaaaataag
    20521 gaaacagagt tatggcactt tggggcgggc gttttaccca ggcagcagat caacggttca
    20581 aacaattcaa cgactcactg cgctttgatt accgtctggc ggagcaggat attgttggct
    20641 ctgtggcctg gtccaaagcc ctggtcacgg taggcgtgtt aaccgcagaa gagcaggcgc
    20701 aactggaaga ggcgctgagc gtgctgctgg aagatgttcg cgccaggcca caacaaatcc
    20761 ttgaaagcga cgccgaagat atccatagct gggtggaagg caaactgatc gacaaagtgg
    20821 gccagttagg caaaaagctg cataccgggc gtagtcgtaa cgatcaggta gcgactgacc
    20881 tgaaactgtg gtgcaaagat accgttagcg agttactgac ggctaaccga cagctgcaat
    20941 cggcgctggt ggaaaccgca caaaacaatc aggacgcggt catgccaggt tacactcacc
    21001 tgcaacgcgc ccagccggtg acgttcgcgc actggtgcct ggcctatgtt gagatgctgg
    21061 cgcgtgatga aagccgtttg caggatgcgc ttaagcgtct ggatgtcagc ccattaggct
    21121 gtggcgcgct ggcgggaacg gcctatgaaa tcgaccgtga acagttagca ggctggctgg
    21181 gctttgcttc ggcgacccgt aacagtctgg acagcgtttc tgaccgtgac catgtgttgg
    21241 aactgctgtc ggctgccgct atcggcatgg tgcatctgtc gcgttttgct gaagatctga
    21301 ttttctttaa caccggcgaa gcggggtttg tggagctttc tgaccgcgtg acttccggtt
    21361 catcattaat gccgcagaag aaaaacccgg atgcgctgga gctgattcgc ggtaaatgcg
    21421 gtcgggtgca gggtgcgtta accggcatga tgatgacgct gaaaggtttg ccgctggctt
    21481 acaacaaaga tatgcaggaa gacaaagaag gtctgttcga cgcgctcgat acctggctgg
    21541 actgcctgca tatggcggcg ctggtgctgg acggcattca ggtgaaacgt ccacgttgcc
    21601 aggaagcggc tcagcagggt tacgccaacg ccaccgaact ggcggattac ctggtggcga
    21661 aaggcgtacc tttccgcgag gcgcaccata tcgtgggtga agcggtggtg gaagccattc
    21721 gtcagggcaa accgctggaa gatctgccgc tcgacgagtt gcagaaattc agcccggtga
    21781 ttggcgaaga tgtctatccg attttgtcgc tgcaatcgtg cctcgacaag cgtgcggcaa
    21841 aaggcggcgt ctcaccgcag caggtggccc aagcgattgc ttttgcgcag gcgcggttgg
    21901 ggtaagaaca tttatatgta caaatttgag cctggcttat cgccgggctt ttttatggca
    21961 aaaaaaagcg gatcctggag atccgcaaaa gttcacgttg gctttagtta ttcgagttga
    22021 gaaactctcg aaacgggcag tgacttcaag ggttaaaaga ggtgccgctc cgtttctgtg
    22081 agcaattatc agtcagaatg cttgataggg ataatcgttc attgctattc tacctatcgc
    22141 catgaactat cgtggcgatg gaggatggat aatgaatatt cgtgatcttg agtacctggt
    22201 ggcattggct gaacaccgcc attttcggcg tgcggcagat tcctgccacg ttagccagcc
    22261 gacgcttagc gggcaaattc gtaagctgga agatgagctg ggcgtgatgt tgctggagcg
    22321 gaccagccgt aaagtgttgt tcacccaggc gggaatgctg ctggtggatc aggcgcgtac
    22381 cgtgctgcgt gaggtgaaag tccttaaaga gatggcaagc cagcagggcg agacgatgtc
    22441 cggaccgctg catattggtt tgattcccac ggttggaccg tacctgctac cgcatattat
    22501 cccgatgctg caccagacct ttccaaagct ggaaatgtat ctgcatgaag cacagaccca
    22561 ccagttactg gcgcaactgg acagtggcaa actcgattgc gtgatcctcg cgctggtgaa
    22621 agagagcgaa gcattcattg aagtgccgtt gtttgatgag ccaatgttgc tggctatcta
    22681 tgaagatcac ccgtgggcga accgcgaatg cgtaccgata gccgatctgg caggggaaaa
    22741 actgctgatg ctggaagatg gtcactgttt gcgcgatcag gcaatgggtt tctgttttga
    22801 agccggggcg gatgaagata cacacttccg cgcgaccagc ctggagacgc tgcgcaacat
    22861 ggtggcggca ggtagcggga tcactttact gccagcgctg gctgtgccgc cggagcgcaa
    22921 acgcgatggg gttgtttatc tgccgtgcat taagccggaa ccacgccgca ctattggcct
    22981 ggtttatcgt cctggctcac cgctgcgcag ccgctatgag cagctggcag aggccatccg
    23041 agcaagaatg gatggctatt tcgataaagt gttaaaacag gcggtttaaa ccgtttaacg
    23101 cagctacccg ataggcttcc gccatggtcg ggtagttaaa ggtggtgttg acgaagtact
    23161 caatagtgtt gccgccacct ttctgttcca taatcgcctg accgatatga ataatttcgg
    23221 cagcgcgctc gccaaagcag tgaataccca gaatctcttt tgtttcccga tggaacaaaa
    23281 ttttcagcgt gcccacgttc atgccgacga tttgtgcgcg tgccagatgt ttaaactggg
    23341 cgcggcccac ttcatatggc actttcatcg cggtcagctg ctgttcggtt ttgcccacag
    23401 agctgatttc cgggatggtg taaatgccgg tagggatatc ttcaatcaga tgcgcggtgg
    23461 cttcgccttt taccagcgcc tgcgcggcaa tgcgcccctg gtcataggcc gctgatgcca
    23521 ggctcggata accaatcacg tcgcccaccg cgtaaacgtg cggctgtgcg gtctgataca
    23581 tgctgttgac cttcagctgt ccgcgactgt cagtttccag cccaatgttc tgtaacgcca
    23641 gcgaatcggt attaccggta cgaccgttgg catagagcag gcagtcagct ttcagttttt
    23701 tacccgactt cagatgcatg atcacaccgt cgtcacagcc ttcgatcttc tcgtactctt
    23761 cgttgtgacg aatcactacg ccactgttcc agaagtgata ggagagagaa tctgacatct
    23821 cttgatcgag aaatgccagc aggcgatcgc gggtgttgat cagatccact tttacatcca
    23881 taccgcggaa gatcgacgca tattcacagc cgatcactcc agcaccatag ataagtacat
    23941 ggcgcggttc gtggtgcatg ctaagaattg agtcgctgtc gtaaatgcgt ggatgggtga
    24001 aatcaacatc tgttggatga tatggacgag agccgcaggc aataacaaat ttttcagcgg
    24061 ttagtgtttc gacgctgccg tccgggcaat ccagcgccaa cgtatgctcg tcaacaaagc
    24121 gagcgttccc ctgcaatatt tcacagtgat tacgttcgta aaatccctga cgcatgcgcg
    24181 tttgttgatt aatcacgtta tcggcatggt taaggatatc ggcaaaagaa gagcggagca
    24241 gtcgcgaatg gtcgctgtat agtgggtttt gattgaattc tataatgcgg ctgacggcgt
    24301 gacggagagc tttcgacggg atggtgcccc agtgggtgca accgccgcca acattttgat
    24361 aacgctcgat aactgcgacg cgcacacctt gcttaaccag gcccattgca gcgccttcgc
    24421 cgccggggcc ggaacctatt actatggcat cgtaatcgta ggaatgtggc atggtagggc
    24481 ttacctgttc ttatacataa aagcaacaga atggtaacat tctatcgcgg gtaagccaat
    24541 tgatccccgt catttatctg gctatatcct gagcggcctt tgctttgtct gtttcttact
    24601 tttgccctga cgttttattg gatttttatc gacgatactc tccgtttaag cggcaggttt
    24661 ccgctgtacg taaaagaacc ggccagtaaa tatgttttat tgcgttaccg ttcattcaca
    24721 atactggagc aatccagtat gttcattctc tggtatagtg ccagcagtac ttttggcaag
    24781 gattcagaca tcgtgatggg cataagagcg caacaaaaag aaaaaacccg ccgttcgctg
    24841 gtggaagccg catttagcca attaagtgct gaacgcagct tcgccagcct gagtttgcgt
    24901 gaagtggcgc gtgaagcggg cattgctccc acctcttttt atcggcattt ccgcgacgta
    24961 gacgaactgg gtctgaccat ggttgatgag agcggtttaa tgctacgcca actcatgcgc
    25021 caggcgcgtc agcgtatcgc caaaggcggg agtgtgatcc gcacctcggt ctccacattt
    25081 atggagttca tcggtaataa tcctaacgcc ttccggttat tattgcggga acgctccggc
    25141 acctccgctg cgtttcgtgc cgccgttgcg cgtgaaattc agcacttcat tgcggaactt
    25201 gcggactatc tggaactcga aaaccatatg ccgcgtgcgt ttactgaagc gcaagccgaa
    25261 gcaatggtga caattgtctt cagtgcgggt gccgaggcgt tggacgtcgg cgtcgaacaa
    25321 cgtcggcaat tagaagagcg actggtactg caactgcgaa tgatttcgaa aggggcttat
    25381 tactggtatc gccgtgaaca agagaaaacc gcaattattc cgggaaatgt gaaggacgag
    25441 taatgaaaca agcaaatcaa gatagaggta cgctgctgct ggcgttagtt gctggcttat
    25501 cgattaatgg tactttcgca gcgctgttta gctccattgt gccattttct gtattcccga
    25561 ttatttccct ggtgctgacg gtttactgcc tgcatcaacg ttatcttaat cgcactatgc
    25621 cggtaggctt gccgggtctg gcagctgcct gttttattct cggcgtactg ctgtacagca
    25681 cggtagttcg tgcggaatat ccggatatcg gctctaactt cttcccggca gtactctccg
    25741 tcattatggt gttctggatt ggcgcgaaga tgcgtaaccg taagcaggaa gttgctgagt
    25801 aatcggtatt atgccggatg ctggcgcatc cggcatgggt tttacttcgc ggtcagtaat
    25861 acgccgcact ccatatggtg cgtgtagggg aactgatcaa acagagccag acgttcgacc
    25921 ttgtgcgtct ggcttaatgt ttccagattc ttgcataacg tttccgggtt acaggagatg
    25981 tacaaaatac acggatacgc ctgcaccatt ttctcggttt cactgtccag accgctgcgc
    26041 ggtgggtcga caaaaatggt ttcgcactga taactcttta agtcgatccc ttgcaggcgg
    26101 ttaaactcgc gcacaccatt catcgcctga gtaaattctt ctgccgccat acgaataatt
    26161 tgtacgttat caatatggtt agctgcgatg ttgtattgcg cagcagcaac cgacggctta
    26221 gcgatttcgg tggctaatac ccgatcaaaa ttgcgcgcca gcgctaatga aaagttaccg
    26281 ttgccgcagt acagctccag taaatcgcct tttgagcctt tggttacgtc cagcgcccat
    26341 tccagcatct gaatattcat cgccgcgttc ggctgggtaa agctgttttc tacctgacgg
    26401 tagatcatct ctttccctgc gaccggcaga cgctcgtcga tgtaatcttg atccagctcg
    26461 attttggttt tcgttgcccg accaatcaga tgcacattca gattctgcgc gcgcagtgca
    26521 tcgcgcaggg cctccgcttc ctgacgccac tcatcatcca gcttcttatg gtatagcagg
    26581 gaaaccaccg cctgattact cagcgtggtg aggtaatcaa tctggaacaa cttgtggcgc
    26641 agaacgggat tattacgcac acccgcaatc atcgccgtca tcaactggtt gataagttca
    26701 ctggcggcgg ggaagctatc cacgcggata cggcttttgg tttgttgatc gaaaatgatg
    26761 tgatacaggt catcgccatc gtgccagatg cggaactccg cacgcatccg gtaatgactg
    26821 accggcgagc gaaacacttc cggaaccagg tcagaaaacg gtgccatcat actttgcaaa
    26881 cgtaccactt tttcggctaa ctgcgcttca tactgttctg ttggaaggtg ttcgggggtc
    26941 atgatgtatc ctgaaaaatt aaagtacgcg gcgattgtag ggattgctca tcagatgtcc
    27001 agatcttgat gaattcctat ttgtgagcta cgtctggaca gtaacttgtt acaacctgta
    27061 gcatccactt gtcggtcctg tgagttaata gggaatccag tgcgaatctg gagctgacgc
    27121 gcagcggtaa ggaaaggtgc gatgattgcg ttatgcggac actgccatcc ggtgggaagt
    27181 catcatctct tagtatctta gatacccctc caagcccgaa gacctgccgg ccaacgtcgc
    27241 atctggttct catcatcgcg taatattgat gaaacctgcg gcatccttct tctattgtgg
    27301 atgctttaca atgattaaaa aagcttcgct gctgacggcg tgttctgtca cagccttttc
    27361 cgcttgggca caggatacca gcccggatac tctcgtcgtt actgctaacc gttttgaaca
    27421 gccgcgcagc actgtgcttg caccaaccac cgttgtgacc cgtcaggata tcgaccgctg
    27481 gcagtcgacc tcggtcaatg atgtgctgcg ccgtcttccg ggcgtcgata tcacccaaaa
    27541 cggcggttca ggtcagctct catctatttt tattcgcggt acaaatgcca gtcatgtgtt
    27601 ggtgttaatt gatggcgtac gcctgaatct ggcgggggtg agtggttctg ccgaccttag
    27661 ccagttccct attgcgcttg tccagcgtgt tgaatatatc cgtgggccgc gctccgctgt
    27721 ttatggttcc gatgcaatag gcggggtggt gaatatcatc acgacgcgcg atgaacccgg
    27781 aacggaaatt tcagcagggt ggggaagcaa tagttatcag aactatgatg tctctacgca
    27841 gcaacaactg ggggataaga cacggggaac gctgttgggc gattatgccc atactcatgg
    27901 ttatgatgtt gttgcctatg gtaatacagg aacgcaagcg cagacagata acgatggttt
    27961 tttaagtaaa acgctttatg gcgcgctgga gcataacttt actgatgcct ggagcggctt
    28021 tgtgcgcggc tatggctatg ataaccgtac caattatgac gcgtattatt ctcccggttc
    28081 accgttgctc gatacccgta aactctatag ccaaagttgg gacgccgggc tgcgctataa
    28141 cggcgaactg attaaatcac aactcattac cagctatagc catagcaaag attacaacta
    28201 cgatccccat tatggtcgtt atgattcgtc ggcgacgctc gatgagatga agcaatacac
    28261 cgtccagtgg gcaaacaatg tcatcgttgg tcacggtagt attggtgcgg gtgtcgactg
    28321 gcagaaacag actacgacgc cgggtacagg ttatgttgag gatggatatg atcaacgtaa
    28381 taccggcatc tatctgaccg ggctgcaaca agtcggcgat tttacctttg aaggcgcagc
    28441 acgcagtgac gataactcac agtttggtcg tcatggaacc tggcaaacca gcgccggttg
    28501 ggaattcatc gaaggttatc gcttcattgc ttcctacggg acatcttata aggcaccaaa
    28561 tctggggcaa ctgtatggct tctacggaaa tccgaatctg gacccggaga aaagcaaaca
    28621 gtgggaaggc gcgtttgaag gcttaaccgc tggggtgaac tggcgtattt ccggatatcg
    28681 taacgatgtc agtgacttga tcgattatga tgatcacacc ctgaaatatt acaacgaagg
    28741 gaaagcgcgg attaagggcg tcgaggcgac cgccaatttt gataccggac cactgacgca
    28801 tactgtgagt tatgattatg tcgatgcgcg caatgcgatt accgacacgc cgttgttacg
    28861 ccgtgctaaa cagcaggtga aataccagct cgactggcag ttgtatgact tcgactgggg
    28921 tattacttat cagtatttag gcactcgcta tgataaggat tactcatctt atccttatca
    28981 aaccgttaaa atgggcggtg tgagcttgtg ggatcttgcg gttgcgtatc cggtcacctc
    29041 tcacctgaca gttcgtggta aaatagccaa cctgttcgac aaagattatg agacagtcta
    29101 tggctaccaa actgcaggac gggaatacac cttgtctggc agctacacct tctgaaccac
    29161 gtcccaccgt gctggtgttt gactccggcg tcggtgggtt gtcggtctat gacgagatcc
    29221 ggcatctctt accggatctc cattacattt atgctttcga taacgtcgct ttcccgtatg
    29281 gcgaaaaaag cgaagcgttt attgttgagc gagtggtggc aattgtcacc gcggtgcaag
    29341 aacgttatcc ccttgcgctg gctgtggtcg cttgcaacac tgccagtacc gtttcacttc
    29401 ctgcattacg cgaaaagttc gacttcccgg ttgttggtgt cgtgccggcg attaaacctg
    29461 ctgcacgtct gacggcaaat ggcattgtcg gattactggc aacccgcgga acagttaaac
    29521 gttcttatac tcatgagctg atcgcgcgtt tcgctaatga atgccagata gaaatgctgg
    29581 gctcggcaga gatggttgag ttggctgaag cgaagctaca tggcgaagat gtttctctgg
    29641 atgcactaaa acgtatccta cgcccgtggt taagaatgaa agagccgcca gataccgttg
    29701 tattgggttg cacccatttc cctctactac aagaagaact gttacaagtg ctgccagagg
    29761 gaacccggct ggtggattct ggcgcagcga ttgctcgccg aacggcctgg ttgttagaac
    29821 atgaagcccc ggatgcaaaa tctgccgatg cgaatattgc cttttgtatg gcaatgacgc
    29881 cagaagctga acaattattg cccgttttac agcgttacgg cttcgaaacg ctcgaaaaac
    29941 tggcagtttt aggctgattt ggttgaatgt tgcgcggtca gaaaattatt ttaaatttcc
    30001 tcttgtcagg ccggaataac tccctataat gcgccaccac tgacacggaa caacggcaaa
    30061 cacgccgccg ggtcagcggg gttctcctga gaatctcaac agagaaaagc aaaaaaatgc
//
LOCUS       DAZPAC010000029        30002 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZPAC010000029 DAZPAC010000000
VERSION     DAZPAC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30002
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.029"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            39..581
                     /locus_tag="ACHUPM_001078"
     CDS             39..581
                     /locus_tag="ACHUPM_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0868924.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            664..1365
                     /locus_tag="ACHUPM_001079"
     CDS             664..1365
                     /locus_tag="ACHUPM_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0868925.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1390..3989
                     /locus_tag="ACHUPM_001080"
                     /pseudo
     CDS             1390..3989
                     /locus_tag="ACHUPM_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3980..5050
                     /locus_tag="ACHUPM_001081"
     CDS             3980..5050
                     /locus_tag="ACHUPM_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0868926.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5062..5604
                     /locus_tag="ACHUPM_001082"
     CDS             5062..5604
                     /locus_tag="ACHUPM_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0868927.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5612..6127
                     /locus_tag="ACHUPM_001083"
     CDS             5612..6127
                     /locus_tag="ACHUPM_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0868928.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6093..6829
                     /locus_tag="ACHUPM_001084"
                     /pseudo
     CDS             6093..6829
                     /locus_tag="ACHUPM_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHUPM_001085"
     CDS             6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHUPM_001085"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR0868929.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8124..8666
                     /gene="zapC"
                     /locus_tag="ACHUPM_001086"
     CDS             8124..8666
                     /gene="zapC"
                     /locus_tag="ACHUPM_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR0868930.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHUPM_001087"
     CDS             complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHUPM_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR0868931.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHUPM_001088"
     CDS             10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHUPM_001088"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR0868932.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12136..14043
                     /locus_tag="ACHUPM_001089"
     CDS             12136..14043
                     /locus_tag="ACHUPM_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0868933.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISHLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQMADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHUPM_001090"
     CDS             14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHUPM_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR0868934.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHUPM_001091"
     CDS             15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHUPM_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR0868935.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHUPM_001092"
     CDS             17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHUPM_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR0868936.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17887..18054
                     /gene="rmf"
                     /locus_tag="ACHUPM_001093"
     CDS             17887..18054
                     /gene="rmf"
                     /locus_tag="ACHUPM_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR0868937.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHUPM_001094"
     CDS             complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHUPM_001094"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR0868938.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18711..20471)
                     /locus_tag="ACHUPM_001095"
     CDS             complement(18711..20471)
                     /locus_tag="ACHUPM_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0868939.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20657..21109
                     /gene="matP"
                     /locus_tag="ACHUPM_001096"
     CDS             20657..21109
                     /gene="matP"
                     /locus_tag="ACHUPM_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR0868940.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHUPM_001097"
     CDS             complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHUPM_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR0868941.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="ACHUPM_001098"
     CDS             complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="ACHUPM_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR0868942.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23308..23937
                     /gene="sxy"
                     /locus_tag="ACHUPM_001099"
     CDS             23308..23937
                     /gene="sxy"
                     /locus_tag="ACHUPM_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR0868943.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="ACHUPM_001100"
     CDS             complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="ACHUPM_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR0868944.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26072..26518)
                     /locus_tag="ACHUPM_001101"
     CDS             complement(26072..26518)
                     /locus_tag="ACHUPM_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR0868945.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26641..28695
                     /gene="helD"
                     /locus_tag="ACHUPM_001102"
     CDS             26641..28695
                     /gene="helD"
                     /locus_tag="ACHUPM_001102"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR0868946.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISSQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="ACHUPM_001103"
     CDS             complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="ACHUPM_001103"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR0868947.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29281..29943)
                     /locus_tag="ACHUPM_001104"
     CDS             complement(29281..29943)
                     /locus_tag="ACHUPM_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR0868948.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         7734 a         7084 c         7878 g         7306 t
ORIGIN      
        1 ggcattatca tatttacgaa gagatcagga tgatgacgat gaaaaaaagt gtattgacgg
       61 cgtttataac tgtggtatgt gcaacgtcca gcgttatggc agctgatgat aatgctatca
      121 cggatggcaa agtaacattt aatggtaaag ttattgctcc agcttgtacc ctggtagctg
      181 cgacgaaaga ttcagtagtc acattgccaa atgtcagcgc caccaagttg caaactaatg
      241 gagcagtctc tggcgttaaa actgatgtac caattgcctt agaaggctgt gatgttactg
      301 taactaaaaa tgcgacgttt acctttagtg gtacggctga cggcgtgcaa cctacagcat
      361 ttgccaacca ggctaccacc gatgcagcga cgaatgttgc tctacaaatg tatctgccag
      421 atggttctac atcggtaact cccggtaccg aaacgtcgaa tattcagtta gcggattctg
      481 ctgagcaaac agtgaccttt aaagttgatt atatcgctac ggggaaagcg acttctggta
      541 atgtgaatgc ggtaacaaat ttccatatta actattatta atagaactca ttaattgttt
      601 tattaattag tacccctcta gtgttctgga ggggatattc atatttttta agagtgacta
      661 tttatgaaaa cttgcataac aaagggaatt gtgaccgtaa gtttaacggc aatattactc
      721 agctgctcat ccgcatgggc ggcaggtaaa ggcgggattg gacttgcagc aacacgtctt
      781 gtttattcag aaggtgaaga gcaaatttca ctgggtgtac gtaacaccag tccggatgtt
      841 ccttatctta ttcagtcatg ggtgatgacc ccagataata aaaaatcagc agattttatt
      901 attacaccac cgctatttgt gctgaatccg gctaatgaga atctgttacg cattatgtac
      961 attggtgcgc cgttggcgaa agacagagaa acccttttct tcactagcgt acgggcagtc
     1021 ccttcaacaa cgaagcggga agagggaaat accctgaaga ttgccacaca aagcgtcatc
     1081 aaacttttct ggcgaccaaa aggtttagcg tatcccttag gcgaggctcc ggcgaaactg
     1141 cgttgcactt cgtcagctga catggttacg gtcagtaacc caacacctta tttcattacc
     1201 ctgacagacc tgaaaatagg gggaaaggta gttaaaaatc aaatgatttc cccctttgat
     1261 aaataccaat tctctctgcc aaagggggcc aaaaatagca gcgtaacgta tcgaaccatc
     1321 aatgattacg gggcggaaac gccacaactc aactgtaaat cgtaagccgt cttcagttaa
     1381 gagagcgaga tgtatagaac tcatcgacaa cacagcctgt taagctctgg tggagtgcca
     1441 tcgtttattg gtgggctggt ggtgtttgtg tcggcagcgt tcaatgcaca agctgaaacc
     1501 tggttcgatc ctgctttttt caaagatgat ccctcaatgg tggccgattt gtctcgtttc
     1561 gaaaaaggac aaaaaataac gccaggggtt tatcgtgtcg atattgttct gaatcagaca
     1621 attgtagata cgcgcaacgt caattttgtt gagataacgc cagagaaggg gattgccgcc
     1681 tgtttgacga ctgaaagcct ggatgcaatg ggtgttaata ctgatgcgtt tccggctttt
     1741 aaacaactgg acaaacaagc gtgtgtgcca ttggcggaga ttattccgga tgccagcgta
     1801 acgtttaatg tgaataaact ccgtctggaa atttcagtac cgcaaatcgc catcaaaagt
     1861 aatgcccgtg gttatgtccc ccctgaacgt tgggatgaag ggatcaacgc gctattactg
     1921 ggatattcgt ttagcggggc taacagtatt catagcagcg cagacagtga ttctggcgac
     1981 agctattttc tgaatttaaa cagtggcgtt aatttaggcc catggagatt gcgcaacaat
     2041 tcaacatgga gtcgcagtag tggccaaacc gcagaatgga agaatctcag cagctatttg
     2101 cagcgggcgg ttattccact aaaaggcgaa ctgaccgtag gtgatgatta tactgcaggc
     2161 gattttttcg atagtgtcag ctttcgtggt gtgcagctgg cgtcagatga caacatgctg
     2221 ccagacagcc tgaaagggtt tgcgcctgtg gtgcgtggta tcgccaaaag caatgcccag
     2281 ataacgatta agcaaaatgg ttacaccatt taccaaactt atgtatcgcc tggtgctttt
     2341 gaaattagtg atctctattc cacgtcgtcg agcggtgatt tgttagttga aatcaaagaa
     2401 gcggacggca gcgtcaatag ctacagcgta ccgttttcca gcgtgccatt actccagcgt
     2461 caggggcgaa tcaaatacgc ggtgacactg gcgaaataca gaaccaatag taatgaacag
     2521 caggagagca aatttgccta ggccacgttg cagtggggcg gaccgtgggg aacgacatgg
     2581 tatggtggtg gacaatatgc tgaatattac cgtgccgcca tgtttggtct gggatttaac
     2641 cttggcgatt tcggagcaat ttcgttcgat gcgacccagg cgaagagtac gctggcagac
     2701 caaagcgaac ataaaggtca gtcatatcgt tttctgtatg ccaaaacgct caaccaattg
     2761 ggcactaatt ttcaattgat gggctatcgc tattcgacgt cgggtttcta caccctttcc
     2821 gacaccatgt ataaacatat ggatggctac gaatttaatg acggtgatga tgaagatacg
     2881 ccgatgtggt cgcgttatta caatttgttt tacaccaaac gtggcaaact gcaggtcaat
     2941 atctcccagc aattaggcga gtacggttcg ttttatttaa gtggtagcca gcaaacttac
     3001 tggcataccg atcaacagga tcggctatta cagtttggct acaacacgca aattaaagat
     3061 ctctcgctgg ggggttcctg gaactacagt aagtcccgtg gtcaacctga tgctgatcag
     3121 gtgtttgcac taaatttttc cctgccgctc aatctgttgc tccccagaag taatgatagc
     3181 tataccagga aaaaaattac gcctggatga cctctaacac cagcatcgat aacgaagggc
     3241 acattacaca aaacctgggt ttaacggaga cactactcga tgacggtaac ctgagctaca
     3301 gcgtgcaaca gggatataac agcgagggga aaacggctaa tggtagcgcc agcatggact
     3361 acaaaggggc gtttgcagat gcccgagtgg gctacaacta cagcgataac ggcagtcaac
     3421 aacaactgaa ctacgctctt tcaggcagtt tagttgccca ttcacagggc attaccctgg
     3481 ggcaatcgct gggggaaact aacgtgctga ttgcagcacc aggcgcagag aatactcgtg
     3541 tggcgaacag caccgggctg aaaactgact ggcgcggata taccgttgtc ccctatgcca
     3601 cttcttatcg ggaaaatcga atcgcacttg atgcggcatc gttaaaacgt aacgtggatc
     3661 ttgaaaatgc agtagtcaac gtggttccca ccaaaggggc gttggttctg gcggagttca
     3721 atgcccatgc gggagcaagg gtattaatga aaacatcaaa gcagggtata ccgctgcgtt
     3781 ttggcgcgat agcgacgctg gacggcgtac agactaatag cggcattatt gatgatgatg
     3841 gctcgctcta tatgtccggt ttgcccgctc agggagcgat aactgtacgc tggggcgaag
     3901 ctcccgatca aatttgtcat atcagttacc agcttaccga acaacaaatt aactctgcga
     3961 ttacgcgaat ggatgccata tgcagataat ctttggagaa aaatgcgtgt tattactacg
     4021 actatttttt gccgccgtct taatgctatg gtgcgctcaa actgctgctt atagcgggca
     4081 gtgtcatacc actcagggga atccgtatat tggcgtcaat tttggcgtta aaaccctgga
     4141 ggaagaagaa aatacggctg gagtagttaa agacaaattt tatcagtgga acgaatcgaa
     4201 tgattattat gtttcctgtg attgcgataa agacaatgtc agaagtggcc gatgggcatt
     4261 cgccgcggat tcaccgttag tctatttagg cgacaactgg tacaaaatta atgactatct
     4321 tgccgccaaa gttttattgc aggttaaagg cagttctcct actgcggttc ctttcgaaaa
     4381 cgtgggcaca ggggcagata cacgatggca tatttgcgat cccggcggtc aacgtttagg
     4441 tggccagggg gctagcggta atagcggtag cttttccctg aaaatattgc agccgttcgt
     4501 tggttcggtc gtcattcctc ctatggcgct ggcgcgatta tttgaatgct acaacatacc
     4561 cgcaggtgat tcctgcacga ctacaggtac accggtttta gtgtattacc tgtctggtac
     4621 gatcaattca cttggctcat gttccgtcaa tgccggagaa acaatcgagg tcgatctggg
     4681 cgacgcattt gcggctaact ttcgtgttgt agggcataag cctcttgggg ccagaacggc
     4741 agaacttgca attccagtca ggtgtaacac gggaaacgcg gggttagtta acgtcaacct
     4801 gagtctgacg gcaaccacag accccagcta tccccaggcg attaagacgt cacgtcctgg
     4861 cgtgggcgtg gtggtgaccg atagccagaa caacattatt tcccctgctg gtggaacatt
     4921 accgctctct attcctgatg atgcagacag tatcgcgcga atgaatgtct atccagtcag
     4981 cacgacaggt gtaccaccag aaaccgggcg atttgaagcc acggcaacgg tgagaataaa
     5041 ttttgattaa cgcgtgaacg tatgaagaaa gaaacgatat ttcagtgcgt tatcttgttc
     5101 tttagcattc ttaacatcca tgtcgggatg gctgggcctg aacaagttag tatgcatatt
     5161 tatgggaatg tggtcgatca gggctgtgat gtcgccacca aaagtgcatt acaaaatatt
     5221 catattggtg attttaatat cagtgatttt caggccgcga ataccgtaag cactgctgct
     5281 gatttgaata tcgatatcac cggttgtgcc gctagtatta ctggcgcgga cgtccttttt
     5341 agcggcgagg ctgacaccct tacgccgaca ctgctcaaac taactgacac aggcggaagc
     5401 ggtggtatgg caacggggat tgccgtgcaa attcttgatg cgcaaagtca gcaagaaatc
     5461 ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac actcaaatat
     5521 caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac ggcggttctc
     5581 tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct ggattctgtt
     5641 cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg tcaacattag
     5701 cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa tcaatgtgca
     5761 gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg agaaatcttt
     5821 catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgacgtca ccttttcagg
     5881 aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg atgcagccag
     5941 cgggctggca attgcgattc tggatgatgc gaagatattg attccgctta accaggcgag
     6001 taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc aattgcgacc
     6061 tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg tacttcatta
     6121 tgactaatac gtggaacaga ttggcgctct tgattttcgc cgttttatcg ctgctggtgg
     6181 cgggtgaatt gcgggcagga gtggtggtcg gcggaacgcg atttatcttt ccggcagaca
     6241 gagaatcgat atctatttta ctgactaata ccagtcagga atcctggctt attaacagta
     6301 aaatcaaccg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca gcaccattac
     6361 tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg cgcttgctga
     6421 gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta agcattgcca
     6481 gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc tcggtattta
     6541 aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat caacaattac
     6601 gctggacacg gaattccagg gtgtacaact cactaaccca acgccttact acattaacct
     6661 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagttgtgc cgcctaaaag
     6721 ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct ggcgggcgat
     6781 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6841 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6901 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     6961 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7021 cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     7081 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     7141 ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     7201 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7261 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7321 aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7381 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7441 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     7501 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     7561 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7621 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7681 acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7741 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7801 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7861 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7921 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     7981 atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct tatttagaca
     8041 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     8101 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     8161 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     8221 gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg
     8281 acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac
     8341 aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc
     8401 cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     8461 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     8521 aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     8581 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca
     8641 gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc
     8701 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc atgacttcgc
     8761 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     8821 gaattccctg attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct
     8881 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     8941 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     9001 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     9061 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     9121 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     9181 acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg
     9241 aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     9301 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     9361 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     9421 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     9481 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     9541 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     9601 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     9661 tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt gtaagaccaa
     9721 tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt
     9781 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     9841 aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt
     9901 gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag
     9961 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
    10021 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
    10081 cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
    10141 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
    10201 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
    10261 agagatgttt aatcctggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
    10321 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
    10381 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
    10441 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
    10501 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
    10561 gatgcgaacc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac
    10621 gttgctgatt gaagcggcga tgctagcgac cgatcgcgca ccaggcttgc accgtgggcg
    10681 ttggggcttt agcggctggg tgcagcatga tgaagctatc tggcaggaag tgaaagcgga
    10741 agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
    10801 cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg ggattggtga
    10861 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
    10921 gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct
    10981 gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct
    11041 ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca
    11101 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
    11161 cactccagac agcaaaccgg cgatggtagc ggaagactac gccaaccgcc tgcgtaagaa
    11221 cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt gaatgttacc gcctgtatga
    11281 cgccgatcta ccggaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    11341 ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat
    11401 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    11461 tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttcttga
    11521 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    11581 gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag gcaaagattt
    11641 cctcaacctg ttctcttata ccggtagcgc caccgtgcat gcgggattag gcggcgcgcg
    11701 cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac gcaacctgcg
    11761 tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    11821 gcgtgaggca aatgaacagt ttgatctgat ctttatcgat ccgccaacct tctctaactc
    11881 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat atggcgctga tgaaagattt
    11941 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    12001 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    12061 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    12121 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    12181 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    12241 ggtcgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    12301 gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca acaggagccg
    12361 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    12421 gaatatctga aacgctatca cgatatttcg cacctggtga tgaacgaccc gagcgagaaa
    12481 aatctcaacg aactggcgaa ggttcaggaa cagttggatc accacaacct gtggcagctg
    12541 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt tgcgctgtcg
    12601 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    12661 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    12721 gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt
    12781 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    12841 gggaattacg accagtacct gcttgaaaaa gaagaagccc tgcgcgtgga agaactacaa
    12901 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    12961 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg tcgcgagcgt
    13021 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    13081 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa gcaactggtg
    13141 aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    13201 tgcggcaaaa ccacgctgct gaaactgatg ctcgatcagc ttcaagcgga cagcgggcgt
    13261 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    13321 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    13381 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    13441 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    13501 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    13561 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    13621 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    13681 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg
    13741 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    13801 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    13861 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcagatggc ggatgcttcc
    13921 ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag
    13981 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    14041 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    14101 ctgggtttat caggcatgaa aaccaacact ttgttagcca tctgtaaaat agcgcatcat
    14161 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    14221 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    14281 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtccgaccgc ctatgcgttg
    14341 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    14401 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    14461 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    14521 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    14581 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg
    14641 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    14701 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    14761 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    14821 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    14881 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcca gcagtggaca
    14941 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    15001 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    15061 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    15121 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    15181 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    15241 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    15301 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    15361 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    15421 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    15481 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    15541 ttacagccat cagggactgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    15601 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    15661 ggctgatgat ttgacgcacg ttgaaatcaa agtgcggctg aattctggta tggaaaaatt
    15721 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    15781 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    15841 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    15901 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    15961 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa
    16021 tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg
    16081 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc gtgtggagat
    16141 gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg aaggtctgga
    16201 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    16261 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    16321 cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa
    16381 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    16441 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    16501 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    16561 ggtcactggt gcactgtatg ttgatcttga tttctatcca aatacgcctg caataaccgg
    16621 tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc tggcgcaaat
    16681 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    16741 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    16801 gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat
    16861 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    16921 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    16981 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    17041 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    17101 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    17161 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    17221 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    17281 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacaattgcg caacaccctg
    17341 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    17401 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    17461 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    17521 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtact ggccagtgtc
    17581 tggagtcagg aagccgcttc tattgcgcaa gagataaagc gtctacctta attataaaga
    17641 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc
    17701 ggaaaaagtt gttcctcttt acattttttg tacaaccgac atacccgtgt agctcacaaa
    17761 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    17821 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    17881 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    17941 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    18001 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    18061 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    18121 taatcagaag gcagaagtat cctggaacag accgactttc aggtcgctgg cggtatagat
    18181 caaacgacca tcaaccagca cttctccatc cgccaggccc ataatcagac gacggttaac
    18241 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    18301 gaatttcact tcgccaacgc ccagtgcgcg gcctttacct tcgccgccca gccagccgag
    18361 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    18421 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    18481 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    18541 catattcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    18601 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    18661 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    18721 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    18781 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    18841 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    18901 ttgccttctt ctaccgcttt cactagttca ctgtgaagac ttaaatggcg aacgttagct
    18961 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    19021 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    19081 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    19141 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    19201 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    19261 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    19321 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    19381 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    19441 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    19501 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    19561 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    19621 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca ggcatccgct
    19681 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    19741 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    19801 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    19861 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    19921 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    19981 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    20041 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    20101 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    20161 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    20221 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    20281 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    20341 aattgcagcc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    20401 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    20461 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    20521 gcactacagc acacgtagaa agcaccgaca atactccagg catgggcgtt aaagctcaca
    20581 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    20641 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    20701 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    20761 tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa
    20821 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    20881 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    20941 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    21001 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    21061 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    21121 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    21181 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21241 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    21301 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21361 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21421 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    21481 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21541 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21601 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    21661 aggaaacccc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    21721 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21781 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    21841 atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag
    21901 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    21961 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    22021 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    22081 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    22141 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    22201 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    22261 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caattaactc
    22321 taccgatatc ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg
    22381 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt
    22441 ttaatctttt ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag
    22501 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    22561 ggattaatcc taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt
    22621 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
    22681 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
    22741 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
    22801 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
    22861 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
    22921 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
    22981 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
    23041 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
    23101 cccctatgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
    23161 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
    23221 cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt
    23281 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
    23341 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
    23401 agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg
    23461 gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa
    23521 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
    23581 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
    23641 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
    23701 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
    23761 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
    23821 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
    23881 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
    23941 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
    24001 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
    24061 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
    24121 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
    24181 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
    24241 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
    24301 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
    24361 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
    24421 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
    24481 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
    24541 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
    24601 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
    24661 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
    24721 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
    24781 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga atgatggcgt
    24841 agccgaagca caataccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
    24901 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
    24961 gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
    25021 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
    25081 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
    25141 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
    25201 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
    25261 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
    25321 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
    25381 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
    25441 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
    25501 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
    25561 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
    25621 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
    25681 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
    25741 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
    25801 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
    25861 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
    25921 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
    25981 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
    26041 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
    26101 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
    26161 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
    26221 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
    26281 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
    26341 cgacatgaat agcttcattg ccataaggca ccagagatag tttagtgatc tcccagcagg
    26401 atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc aacagccagc
    26461 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
    26521 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
    26581 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
    26641 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
    26701 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
    26761 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
    26821 ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt
    26881 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
    26941 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
    27001 acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg
    27061 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
    27121 gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc
    27181 atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg
    27241 caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc
    27301 ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc
    27361 cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgg
    27421 attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
    27481 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc
    27541 gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg
    27601 caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt
    27661 aacttctggg atgatgaaaa attacagcgt cgccttgcct ctcgcctcga tcgctgggta
    27721 agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag
    27781 attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa
    27841 ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
    27901 attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag
    27961 gatatctcgt cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg
    28021 acgttgttcg ctgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg
    28081 tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg aacgttgtga tttagacacg
    28141 acttaccgtt ttaacagtcg tatcggtaag gtggcaaacc ggtttattca gcagaaccca
    28201 ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc cgtcacgtta
    28261 ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
    28321 gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg
    28381 gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa
    28441 caggcggatt acgtcatcat cgttggcttg caggagggaa gtgatggttt tccggctgcg
    28501 gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct
    28561 gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt atgggcactg
    28621 tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggtg
    28681 gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt
    28741 ccgcgagata acgctgataa tcggggatta gaatatcgac cgcgtcgttg aaatgcggcg
    28801 actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg
    28861 ccagacgcag caaggctttc acgtcaggat cgtgcggcac ggcatttagt ggatcccaga
    28921 agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc
    28981 ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac
    29041 cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc
    29101 agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag
    29161 gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac
    29221 tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc
    29281 tcaggaagat ggttgttttt ccgcccactg caggaaagta tttcgcgttt gtgggtcagc
    29341 cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc
    29401 atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg
    29461 cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat
    29521 cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc
    29581 gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc
    29641 caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag
    29701 cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg
    29761 aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga
    29821 gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt
    29881 ggtggcaaaa acggataccg gcaaacataa tgcaattaag gtggtcacga tgccggtttt
    29941 cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat
    30001 ca
//
LOCUS       DAZPAC010000030        29757 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZPAC010000030 DAZPAC010000000
VERSION     DAZPAC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29757
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.030"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(99..326)
                     /locus_tag="ACHUPM_001105"
     CDS             complement(99..326)
                     /locus_tag="ACHUPM_001105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868949.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            complement(351..2984)
                     /gene="mngB"
                     /locus_tag="ACHUPM_001106"
     CDS             complement(351..2984)
                     /gene="mngB"
                     /locus_tag="ACHUPM_001106"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR0868950.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(3002..4918)
                     /gene="mngA"
                     /locus_tag="ACHUPM_001107"
     CDS             complement(3002..4918)
                     /gene="mngA"
                     /locus_tag="ACHUPM_001107"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR0868951.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            5087..5809
                     /gene="mngR"
                     /locus_tag="ACHUPM_001108"
     CDS             5087..5809
                     /gene="mngR"
                     /locus_tag="ACHUPM_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR0868952.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(5913..6782)
                     /gene="sucD"
                     /locus_tag="ACHUPM_001109"
     CDS             complement(5913..6782)
                     /gene="sucD"
                     /locus_tag="ACHUPM_001109"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR0868953.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(6782..7948)
                     /gene="sucC"
                     /locus_tag="ACHUPM_001110"
     CDS             complement(6782..7948)
                     /gene="sucC"
                     /locus_tag="ACHUPM_001110"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR0868954.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(8042..9259)
                     /gene="odhB"
                     /locus_tag="ACHUPM_001111"
     CDS             complement(8042..9259)
                     /gene="odhB"
                     /locus_tag="ACHUPM_001111"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR0868955.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(9274..12075)
                     /gene="sucA"
                     /locus_tag="ACHUPM_001112"
     CDS             complement(9274..12075)
                     /gene="sucA"
                     /locus_tag="ACHUPM_001112"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR0868956.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(12376..13092)
                     /gene="sdhB"
                     /locus_tag="ACHUPM_001113"
     CDS             complement(12376..13092)
                     /gene="sdhB"
                     /locus_tag="ACHUPM_001113"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR0868957.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(13108..14874)
                     /gene="sdhA"
                     /locus_tag="ACHUPM_001114"
     CDS             complement(13108..14874)
                     /gene="sdhA"
                     /locus_tag="ACHUPM_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR0868958.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(14874..15221)
                     /gene="sdhD"
                     /locus_tag="ACHUPM_001115"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(14874..15221)
                     /gene="sdhD"
                     /locus_tag="ACHUPM_001115"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR0868959.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(15215..15619)
                     /gene="sdhC"
                     /locus_tag="ACHUPM_001116"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(15215..15619)
                     /gene="sdhC"
                     /locus_tag="ACHUPM_001116"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0868960.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            16000..16107
                     /locus_tag="ACHUPM_001117"
     CDS             16000..16107
                     /locus_tag="ACHUPM_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0868961.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16313..17596
                     /gene="gltA"
                     /locus_tag="ACHUPM_001118"
     CDS             16313..17596
                     /gene="gltA"
                     /locus_tag="ACHUPM_001118"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR0868962.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            17986..18552
                     /locus_tag="ACHUPM_001119"
     CDS             17986..18552
                     /locus_tag="ACHUPM_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0868963.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            18708..20740
                     /locus_tag="ACHUPM_001120"
                     /pseudo
     CDS             18708..20740
                     /locus_tag="ACHUPM_001120"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            20717..21061
                     /locus_tag="ACHUPM_001121"
     CDS             20717..21061
                     /locus_tag="ACHUPM_001121"
                     /inference="COORDINATES: protein motif:HMM:NF025319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFR0868964.1"
                     /translation="MNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGKDVNVIIRNA
                     SGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQHCSLHLPEHM
                     EDTANRLILPCH"
     gene            21076..21807
                     /locus_tag="ACHUPM_001122"
     CDS             21076..21807
                     /locus_tag="ACHUPM_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0868965.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            21804..22865
                     /locus_tag="ACHUPM_001123"
     CDS             21804..22865
                     /locus_tag="ACHUPM_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0868966.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            23017..24063
                     /locus_tag="ACHUPM_001124"
     CDS             23017..24063
                     /locus_tag="ACHUPM_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR0868967.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(24060..24851)
                     /gene="nei"
                     /locus_tag="ACHUPM_001125"
     CDS             complement(24060..24851)
                     /gene="nei"
                     /locus_tag="ACHUPM_001125"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR0868968.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(24887..25621)
                     /gene="pxpA"
                     /locus_tag="ACHUPM_001126"
     CDS             complement(24887..25621)
                     /gene="pxpA"
                     /locus_tag="ACHUPM_001126"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR0868969.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(25611..26543)
                     /gene="pxpC"
                     /locus_tag="ACHUPM_001127"
     CDS             complement(25611..26543)
                     /gene="pxpC"
                     /locus_tag="ACHUPM_001127"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFR0868970.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(26537..27193)
                     /gene="pxpB"
                     /locus_tag="ACHUPM_001128"
     CDS             complement(26537..27193)
                     /gene="pxpB"
                     /locus_tag="ACHUPM_001128"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR0868971.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(27216..27959)
                     /gene="ybgI"
                     /locus_tag="ACHUPM_001129"
     CDS             complement(27216..27959)
                     /gene="ybgI"
                     /locus_tag="ACHUPM_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR0868972.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            28230..29711
                     /gene="dtpD"
                     /locus_tag="ACHUPM_001130"
     CDS             28230..29711
                     /gene="dtpD"
                     /locus_tag="ACHUPM_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR0868973.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         7147 a         8005 c         7298 g         7307 t
ORIGIN      
        1 tctttttttg gtcactggct aatacgacgc agagtttcag gattactaat aaaggcataa
       61 atccaccccg ccgatattaa catttaatta acttgtaatt atattataat taactcaccc
      121 agggttatta ttttgttaaa aataaagcca catatacgtg gcttgagagt gaagataaac
      181 gcaaaattac ctgatgcgct acgcttgcca gtgtctacat catctctgca atatattgaa
      241 tttgcctgct tttgtagacc gaataaagcg ttcatgccgt atccggcatg aacaaagcgc
      301 cctttgtcaa caatctggcc gcgcatatgt ggcttcggtt tgattgctat tcaggcaagc
      361 cggtaactga acgtccgtga ctggccgggt aaaaaaggcc ccgatagatt tgaaccttga
      421 ttttcctcgg tggtaatgtg ttcatccatc atcgtttctg agcaagaaat cacctcgcga
      481 ctgaaagcaa cagtcgcatc acaggttgct gattcagccg gattaaacag ccgtaaaatc
      541 acttcttgtc ggtcttcagc tttcttaagt gcgcttatca ggcatcccac tgggggcatt
      601 ttcaacaaac tataactttc cggcacgttg aatccggctt tgttgagctt catcgcatcc
      661 catgggattt tgttgtaaca ctgtactgga gtcagccatg ctcgcgcctg ctgagctaca
      721 ccagcggcgg ttggcgtacc ggtataactc aataaactta ggcgacaaga aagcagacca
      781 cgtagttgtg agtccgggac tggcatttta attcccgaag gccgcccagg ccttaaaagc
      841 agatcttctt tgcccagtaa gcccacgcca cgcagcaacg taatggcaaa ggttttcttc
      901 tcttcaccga tgacttcaaa ttcacgtaac ccttcgctaa agacagccat gccgttacgc
      961 ccttcctgta aggcaacata gttgagcata ttccataccg gaaccggcgc ttctttccag
     1021 ccttcttgct gccagttgtt cattgcactg tcgttcacgg ggcgcgttag cgaaccaaac
     1081 tgcgtatctg ccagaacact gtcagtgtta aaaggtgtag ggaccaggac acgaaggcga
     1141 tgatcgtcag cctggttatc aaggttgata tccacatcaa tacgcctgct gttatgacta
     1201 agagtgacaa ccaacacccc ccctactctg ccagtggatt gccgggcgct gcgttctgac
     1261 aaattgagcg gcactgccat gtcatagcgg ataacagccc tgctctgcca ggcttcatga
     1321 ataatatcgc attgcggttt cgcgttcgct gcggtaatta cccactcttc ttttgcgggt
     1381 gaatagtcat attcatcacc atcatcagag ctttcttcaa tttgcaatac ccggtcatag
     1441 cgcacaccgc tgtctttatc taccagttgc agagaaccat cctcattgag cgcaatctgc
     1501 cagaaagcat tttccagaat cccttcagcg tcactttttg ccgcaatcac gttgccaggc
     1561 tgattcgctt caatataaag cgtgcgatag cccatagaag ggacaatctg gttgatctga
     1621 atatcaaact ccataaaggg atcataattg ccataatgaa caatttgccg atcgattagg
     1681 ccaggatcga tctcacgggc atggcgaata aaatacggta caggctgacc gcgatcgtct
     1741 tgtagattaa actggctggc gcgcagccgc acagtggtgt taataacttc ttcccgcggc
     1801 cagggcatca ggttaaacag cacgagtttg tcggcatcac tctgtggcat gttatcggca
     1861 attttgcgca tgtagaaacg cagcagatta tccgccatgt cttcagccag ttcgaagcgg
     1921 gcgacaattt cgcgatgaac tttgtcactg caacaacaac cgatactgtc gtgggcatga
     1981 ttttttaaga tctctttcca cattttctcc agcaagccgt ggtggtattc aaaacccaac
     2041 gtccaggcca gcgttgccag cggttccagc agattaacaa tcttattttc aatacgtgcg
     2101 tgggcaattt tgatatccat gcgcgtggaa ccgatggtgc gatgtacgcg catatatttg
     2161 ccatcgataa attcaccttt cagggtggcc agactttcac gctgcgcttc gatcttctca
     2221 aatacctctt caaagcggct catcacaaac ttacgttgag ggtagatctc acgtagctta
     2281 tccatcactt cgaagatatt ttgctgcaat ggcatctggt catgaccatt cggcagcaaa
     2341 atctctttgg ttacagacgc tttttccagc acgtcaaaat aactgtcgag gcgtttacgt
     2401 aatccgtttt cgtcggcagg taagtactta ccgatggcgt agcccagcgg cagcacctgc
     2461 gccgtcactt cgctaccgtc actgctttgc cacaaaaact cggttttatc agtaccgtgg
     2521 cgctccgaac atccgcgcca gaacatagtg cgggtaatgc caaatccatt gtaaatatgc
     2581 ggcagttgcc cggacatgcc aaaggaatcc ggtaaataac ctattttcat cggctcgcca
     2641 aacgcgaggc agtcacgcat tccgtacatc agattacgga cgatggattc cgcagaaaca
     2701 atcgtggtat cggtctgggt ataccagggg ccgataatca acttaccggc ttctacctgt
     2761 ttcttcacgc ggtctttgtt ttccggtttc accgcgaaat aatcttcgag gatcgccgtt
     2821 tgcccgtcga gtacgtaata tttgtattcg ttgtcctgtt ccagtcggca caggatctct
     2881 tccatattat tgaccagcag aatgcgtgac tcttcggtgg tgaaatacca ctcccgatcc
     2941 caatgcatat gcggggtgat gtgaacgcga gataccgctt tcatcgtcgt ttcctttttg
     3001 tttatggcat tacgccatca gtcagatagt tgccatgctt aaccgcgtga cgccgccaga
     3061 tcaggagaat tgcagtcgag attgcagccc ccaccaatgc cgcaccaaac cagccaattg
     3121 ccgccataac accgcccgcg ccattatcat gaagtaaaaa gagcgagaaa atgccggcac
     3181 cgggtgtcga aaggccgata ttcatcgcac cgacaatagc gcccgttacc atagagccca
     3241 gcacaaacga accaataacc cgcagcggat cttcaatcgc catcgggatc gccccttcgg
     3301 taatacctgc cagccctaac agccaggtgg atttcccggt ctcaatttca aactctttaa
     3361 acaggcgcgg tgcgagcatc gtggaagcgg ttacggtaaa tgccgaaacc attttgacgg
     3421 aggcgaaaat ggcatacggg ccgtaaacgc cgttcgccat tgcgcccagg cagaatgcat
     3481 aagcggcttt gttcactggc cctccaaggt caaaggaaca cataaaaccg agaatggcac
     3541 ccagcaacag cgcgttactt cctgacagac cgttcagcca ggcggtaagc gagttattga
     3601 tccatgcgac aggttctccc accacaaaca gcatcagact gcccgctccc aacgtaccga
     3661 gcaccgggta gagataaaaa gtcaggaatc cattgaattt actgctaaga cgcaagtgat
     3721 ttttcaccca gcgcatcaag taaccggcta tcaatccgcc aacgaccgcg ccgagaaacc
     3781 cggagccgat catgttggcg gcaagtccag ccgcaaagcc tggcgctaac gccggtttat
     3841 ctgccagaga ataggtggta taggccgcga gcaccggtac catcaaaatt ccgagcagcc
     3901 cgccgcccag cttgcggtac atccacagcc aggagttttc ttcattaaac agatcttgta
     3961 gcccgaagat ttgcgacagt aataccgcga ccgccagcac cgtgcccccc gcgacaatca
     4021 acggtacggc aaaagagatt ccgctcaaca gtgcctgttt cagctccgtt ttgacacttt
     4081 tcaccggttg cgtatcttgc tgtacggtac gcgtctcatc gctacgcttg agggtaagcg
     4141 cttgttggat caacgcttct gcatggcgaa tcggctcggc aacaggcact gaaagcgcgg
     4201 gaatgccatt aaaacgctca ctctccttga tggcgacttc agccgcaaaa atacaggcgg
     4261 ttgcactatt gagttgatcc gccgttaaac gcccttcaat gccgttagcg ccttgttttt
     4321 caacgtaaac atttacgccg agtttgcgtc cggctttttc cagatattcc gcagccatat
     4381 aggtgtgagc aatacccgcc ggacaggccg ttacgcagac gatagttggc gcgttggaaa
     4441 aagaggcaga aggttgcgtg cctcccttgt catccagcgc cgagagcaac tcatcaggcg
     4501 tcgtcgccga ctgtatacgc gcccgaatct catcatccgc aaggcgcgtg gtcagcgctg
     4561 tcagcagttg catatgcgta gtacccgctt cattgggggg aatcgccagc agcaccacta
     4621 aatcaactgc ttccgggcca tcaacgcctt cccactgaag cggctcgctg agtgtggcga
     4681 ccgcaaacgc cgcttctttt accgcagcag ttttgccatg cggcacagcc aacccttcac
     4741 ctaaggcggt cgggccaagg ctttcacggc gatacacttc ttccagaaat tgctcagtac
     4801 tggaaatttt ccccagagca gcaagacgtt gagtcaacgc gtggatggcc tcttcacggc
     4861 tggtaaagcg cgcattcaga cacaacgcat cgcggtgggt cagagtcgtc agattcatca
     4921 tgatcctcac ttgatcgttt ttatagtcgc gccagtgtgc ccgataaaac aataccatca
     4981 tgtgatcaat ttcacttgat tgaaattatt tgtattgtat ttgtattatt tacaggatga
     5041 atttatcggg cataatggct aaattcatat taatgagata ctaaaaatgg gacacaagcc
     5101 cttataccgg cagattgccg atcgcattcg tgaacaaatt gcgcgtggcg agcttaaacc
     5161 tggcgatgcg ttacccactg aatcagcgct acaaacagag tttggcgtca gccgggttac
     5221 ggtgcgtcag gctttgcgcc agctagtcga gcagcagatc ctcgaaagca ttcagggcag
     5281 cgggacttac gtcaaagaag agcgggtcaa ttacgatatt tttcagttaa ccagttttga
     5341 tgaaaaactg tcggaccgtc acgtcgatac acacagtgaa atcctgatat ttgaagtgat
     5401 tccggctgac gattttcttc agcaacagct acaaatcacc gcgcaggatc gtgtgtggca
     5461 tgtgaagcgc gtgcgctatc gcaagcaaaa gccaatggcg ctggaagaaa cctggatgcc
     5521 gcttgcgttg ttcccggatc tcacctggca ggtcatggaa aattcgaaat atcactttat
     5581 tgaagaagtg aagaagatgg ttatcgatcg tagcgaacag gaaatcattc ctctgatgcc
     5641 aaccgaagag atgagccgtc tgcttaatat cagccagaca aagccgattc ttgaaaaagt
     5701 atcgcgtgga tatttagttg atggtcgggt gtttgaatat agccgcaacg cttttaatac
     5761 cgatgactat aaatttaccc tgatagcgca gcgaaaatca tcgcgataag cacaaaaaag
     5821 gccatcatac gatggccaac catgtcgaaa ccgacagccc ttcgccattc ctgtagaggg
     5881 aagcggcgag ggctatttct tattacagat atttatttca gaacagtttt cagtgcttca
     5941 ccgatatccg ccaggctgcg aacggttttc acgcctgcgg cttccagagc agcgaatttc
     6001 tcatccgcag tccctttccc accagcaatg atcgcacccg cgtggcccat acgtttgcct
     6061 ttcggcgcag tcacaccagc gatgtaacct acaactggct tggtaacgtg ctctttgatg
     6121 tacgctgccg cttcttcttc agcgctaccg ccgatctcac cgatcatcac gatcgcttcg
     6181 gtctgcggat ctttttcgaa catttcgagg atgtcgataa agttagagcc tgggatcggg
     6241 tcaccgccga taccaacaca ggtcgactga ccgaaaccgt aatccgtggt ctgtttaacc
     6301 gcttcatagg tcagtgtacc ggaacgggaa acgataccca ctttacccgg tttgtgaatg
     6361 tgacccggct ggataccgat tttgcattca cccggagtga taacgcccgg gcagttcggg
     6421 ccgatcatac gaacgccggc ttcgtccagt ttcactttca cagtcagcat atccagcgtc
     6481 gggatgcctt cagtgatggt gataatcagt ttgatgcctg catcaatagc ttccagaatg
     6541 gagtctttgc agaacggtgc tggtacgtag ataacagaag cggtagcgcc agtagcagca
     6601 acagcttcac gcacggtgtt gaacaccggc aggccgaggt gggtagtacc gcctttacct
     6661 ggggttacgc cgccaaccat tttagtgccg tatgcaatgg cctgttctga gtggaaagtc
     6721 ccctggctac cagtaaagcc ctggcagata accttggtgt ttttatcaat taaaatggac
     6781 attatttccc ctccactgcg gcaacaacct gctgagctgc atccgtcaga ccttttgctg
     6841 caataatatt caggccgctg tcagccagtt tcttcgcgcc gagttcggcg ttgttacctt
     6901 ccagacgtac cacgaccggt acgttaacac ccacttctgc taccgcgccg atgataccgt
     6961 cagcgatcag gtcgcaacga acgataccgc cgaagatgtt aaccagaacg gctttcactt
     7021 tgtcgtcaga gaggatgatt ttgaacgctt cggttacacg ttctttggtt gcgccgccgc
     7081 caacgtcaag gaagttagcc ggttcgccgc cgtgcagttt aacgatgtcc atcgtaccca
     7141 tcgccagacc tgcgccgtta accatacaac cgatgttacc gtccagcgca acgtagttca
     7201 gttcccactg tgcagcctgt gcttcacgcg gatcttcctg cgactggtca cgcatttcgc
     7261 gcagatcagg ctggcggaac agtgcgttgc cgtcagcgcc cagtttgccg tcgaggcaaa
     7321 tcagatcgcc ctgtttggtg atgaccagcg ggttgatttc gatcaacgcc aggtcgcgct
     7381 ccaggaaaat ggtcgccagg cccatgaaga ttttggtgaa ctgctgaacc agtttacctt
     7441 ccagacccag tttgaacgcc agctcgcgtc cctgatacgg catcgggcca gtcagcggat
     7501 caagcgcaac tttgtggatc aggtgcggag tttcttccgc cactttttcg atttccacgc
     7561 cgccttcggt ggaggccata aagaccacac gacgggaact acggtctaca acggcaccga
     7621 gatacagctc tttagcgata tcggtcgctg cttcaaccag aatctggtta accggttggc
     7681 cattggcatc tgtttgatac gttaccagac gcttgccgag ccagttttct gcaaaagcac
     7741 ggatgtcttc tttgctgttt acaactttca caccgcccgc tttaccgcgg ccaccagcgt
     7801 gaacctgaca tttcactacc cacgggccgg caccgatttt tgaagcggct tcttctgctt
     7861 cgcgcggagt agtacaggca taacccaccg gtgctggtaa gccatagcgg gcaaaaagtt
     7921 gttttgcctg atattcatgt aagttcatgt gttctgtcca tccttcagta atcgttatct
     7981 tttaaaccgt agaccgacac cctatccacg tcggtctaca gtgcaggtga agcttaaact
     8041 actacacgtc cagcagcaga cgcgtcggat cttccagcaa ctctttgatc gttaccagga
     8101 agcccacgga ttcgcgacca tcgatcagac ggtgatcgta ggacagcgcc aggtacatca
     8161 tcggcaggat ctcaacctga ccattcaccg ccatcggacg atctttgata gcgtgcatac
     8221 ccagaattgc gctctgcggc gggttgatga tcggcgtaga catcagggaa ccgaacacac
     8281 caccgttggt gatggtgaag ttaccaccgg tcagatcttc aaccgtcagc ttgccgtcac
     8341 ggcctttaac tgccagctct ttgattttct tctcgatgtc tgccatgccg agggtatcga
     8401 catcacgcag taccggcgtc accaggccgc gcggcgtgga aaccgccatg ctgacgtcga
     8461 aatagttgtg gtaaaccacg tcatcaccgt cgatagaagc attcacttcc gggtaacgtt
     8521 tcagggcttc aaccaccgct ttcacgtaga aggacataaa gcccagacgg atgccgtggc
     8581 gtttttcaaa cgcttcaccg tactgcttac gcagatccat aatcggcttc atgttgactt
     8641 cgttgaacgt ggtcagcatg gcggtggagt ttttcgcttc cagcagacgc tctgccacac
     8701 gcttacgcag gcgagtcatc gggacacgtt tttcactacg tgcagccaga gccggttgcg
     8761 ccgccggagc agccgctgcc ggagcagact ctttcgccgg ggctttcgcc agatgttttt
     8821 ccacatcttc acgagtcaga cgaccaccca caccggtgcc tttaatggcg ctggcgtcga
     8881 gattgtgttc agccagcagg cgacggatcg ccgggcttaa cgcatcgttg ttttgctctt
     8941 ccagagacgc ctgctggcgt tgcgccggag tggacgcttt ctcttcagat ttggcgctgg
     9001 tttctttacc ggcgctgttg ccttcacgca ggcgaccaag gatctgacga gacgttaccg
     9061 ttgtaccttc atcttccaga accgcatcca gaatgccgtc tgctgatgcc ggtacttcca
     9121 gtaccacttt gtcagtttcg atttctacca gcacttcatc acgtacgact gcgtcgccgg
     9181 gttttttatg ccaggttgcg acggtggcat cggctacgga ttcaggcagg tcagggacca
     9241 gaatatctac gctactcatt gtgtatcctt tatttattcg acgttcagcg cgtcattaac
     9301 cagatcttgt tgctgtttct ggtgaacgga catataccct accgccggag aggcggaggc
     9361 cgtgcggcct gcataacgca gagaagcccc aaacggaatc acttcacgga aatgatgctg
     9421 gctgcagtac catgcgccct ggttgagcgg ctcttcctgg caccagacaa aatccttgac
     9481 gtgagcaaac tgctgcaaca cttcctgcat cgctttatgc gggaacgggt agagttgctc
     9541 gatacgcaca atggcgacat cgtgttgatt gttcttacga cgctgttcca gcaggtcgta
     9601 ataaacctta ccagaacaca tcactacgcg cttcacgccc ttcggatcaa gctcgtcgat
     9661 ttcaccgatg gctggcagga aggtgccgtt cgccagttct tcgaggctgg aaaccgccag
     9721 cggatgacgc agcagggatt tcggcgacat cactaccagc ggacgacgca tcccgcgcag
     9781 cgcctgacga cgcagcatgt ggtaaacctg tgccggggta gacggaacgc aaacctgcat
     9841 gttttgctca gcacaaagtt gcagataacg ttccagacgc gcggaggagt gctccggccc
     9901 ctgcccttcg taaccgtgcg gcagcaacat caccagacca cacatccggc cccatttctg
     9961 ttcgccagaa gagatgaact ggtcgataac cacctgcgca ccgttggcga agtcaccgaa
    10021 ctgcgcttcc cagatggtca gagtgcgtgg ttctgcggtg gcataaccgt attcaaacgc
    10081 cagcactgct tcttcagaca gtacggagtc ccagacgcgg aacgcgccct gcccgttatg
    10141 gatgtgttgc aacggcgtgt aagtggaacc gttagactgg ttgtggatca ccgcgtggcg
    10201 gtggaagaag gtaccgcgac cggagtcttc acccgacagg cgaaccggaa tgccttcgtc
    10261 aaccagcgtg gcgtaagcga ggttttccgc accgccccag tcgaacagtt tctcaccggc
    10321 agccatcgcc tggcgatcgc cataaatctt ggcaacgcga gactgcattt caactgcttc
    10381 cggcaccgtg ctgatgcgtt tcgccagctc ctgcaggcgc ttcatctcaa ctttgttcgg
    10441 gtactcttcg tcccattcgt ggttgaggta cggcgaccag gtgaaagagt gcatgttcat
    10501 cggacgccac tctgccacta cgcaatcgcc agcatccagc gcatcgcggt acaggttaac
    10561 catctcggtg gcatcttcca gcgtcgccac tttttcctgc tccagcttgt cagcgtagat
    10621 tttgcgcggt gtcggatgtt ttttgatttt ctgatacatc agcggctggg ttgcgctcgg
    10681 ctcgtcggct tcgttgtggc cgtgacggcg gtagcacacc aggtcgatga agacatcacg
    10741 tttaaaggtg ttacggaaat cgagcgccag acgggtcaca aaggcaacgg cttccggatc
    10801 atccgcgtta acgtggaaaa tcggtgcctg aaccatctta ccgatatcag tacagtacgg
    10861 tgtagaacga gcatccagtg gattagaggt ggtgaaacca acctggttgt tgataacgat
    10921 acgtaccgta ccgccaactt cataaccacg cgctttcgac atgttcagag tttcctgaac
    10981 cacgccctgc ccggtcactg cggcgtcacc gtggatggtg attggcagca ctttgttgct
    11041 gctcggctca tcaagtctgt ccagacgggc acgaacagaa ccgataacta ccgggcttac
    11101 aatctcaagg tgagacgggt taaacgccag cgccaggtgc accaggccgc catcggtctg
    11161 gaagtcagac gagaagccca tgtggtattt cacgtcaccc gtgccgaggt gttctttatg
    11221 tttaccggca aactcgtcga acaagtcttg cggtttttta cccagcacgt tcaccagcac
    11281 gttcagacga ccacggtgcg ccatcccaag aaccacttcg cgggtgccgc tgttgccagc
    11341 gtggcggatc atctctttaa gcatcgggat taacgcgtca ccgccttcca gcgagaagcg
    11401 ttttgcgcca gggaattttg cgccgaggta acgttcaagg ccttcagcgg cggtcagttc
    11461 gcttaagaag cgttttttct cttcgctatt gaaagtcgcg cgaccagact cgatacgctg
    11521 ttggatccag cgtttttctt cggtgctggt aatgtgcata tactcggcac caatcgggcc
    11581 acagtaggtt tgcttgaggg cttccagcag ctcgccgagt ttcatgtttt ctttgccgct
    11641 ggcaaatgaa ccgacgttga aggtctcctg gaagtctgct tcggtcagat cgtggaaaga
    11701 cggatccaga tcggccactt tatcttgctg ccacagtccc agcggatcga gattcgcatg
    11761 ctggtgacca cggaagcggt atgcgttaat gagctgcagg actttaacct gcttcacatt
    11821 ggtgtcaggg tcggagatcg ttgaagagta acgtgaagcg tctttcgcca ggcggcggaa
    11881 atattcacgc gtttgagagt ggaattgatc cggtttgact cccgtaccag gtaactgctg
    11941 gaacgtcgaa cgccagttag cgtcaaccga gtcaggatcg gttaagaagt cttcatagag
    12001 ctgttctatc cagctctggt ttgcgccaga gaggtaagaa gagtccaacc aggctttcaa
    12061 agcgctgttc tgcatcgtga tcccttaagc atctttttta tgcttacttc gccgtggata
    12121 ctaccacgca cagtgatgtg cgtgccgggg ttcacttgcg agctcttgtc tatgtagtgg
    12181 ctcgcgaagg aacctttaaa aactgtctta gtgtcagggg cagtttttaa agatttcctg
    12241 catttctttg ttacgcctga tgcgctgcgc ttatcaggcc tacgggtaat gacttgtagg
    12301 ccggataagg cgttcacgcc gcatccggca ctggttgcct gatgcgacgc ttgcgcgtct
    12361 tatcaggcct acggtttacg cattacgttg caacaacatc gacttaatat ggccgatggc
    12421 gcgcgtcggg ttcagcccct tcggacatac actgacgcag ttcatgatgc tgtgacagcg
    12481 gaatacgctg aatgcatcgc tcaaaccgtc gaggcggctg tcagtctcgg tatcacggct
    12541 atcgatcagg aaacgatatg ccgctaacaa gcctgccggg ccgataaact tatcgggatt
    12601 ccaccagaaa gacggacaag aggttgaaca acatgcgcag agaatacatt catacagccc
    12661 gtcgagtttt tcgcgctgct ctggcatctg taaatgctcg cgagctggcg gattttgtcc
    12721 attattcaac aggtaaggct taattttctc atattgcgca tagaattgtc ccatgtctac
    12781 caccaaatcg cggatcaccg gtaaacctgg cagcgggcga atcacaatct tcttgcccgg
    12841 ctggttgagt gccgaaatcg gggtaataca ggccagacca ttcttgccgt tcatgttcag
    12901 accgtcggaa ccgcacacac cttcacggca ggagcggcgg aacgacaggc tgggatcttt
    12961 ctctttcagc tggataagcg catccagcag catcatgtcg cgaccttctt ccgcttccag
    13021 ggggtaatcc tgcatacgcg gagcatcatc aacatccggg ttatagcgat aaattgaaaa
    13081 ctcgagtctc attttcctgt ctccgcatta gtaagtacga atcttcggcg ggaatgccgg
    13141 gcgcagtttc ggttccatgt tgacgcttcg gcgcgtcatg gattccgact ctggcagata
    13201 cagggagtgg cacagccagt tttcatcatc acgatccggg aagtcgaagc ggctatgtgc
    13261 gccacggctt tcggtacgga agttggcaga aacagccgtt gcatacgccg tttccatcag
    13321 gttatccagt tccaagcact caacgcgctg ggtgttgaac tcgctggaag tgtcatccag
    13381 acgggcattt ttcagacgct cgcggatcac tttcaactgc tcaagccctt tcgccatcgc
    13441 atcaccttca cggaagaccg agaagttatg ctgcatacat tcttgcagcg ctttacgaat
    13501 cgccaccgga tcttcaccgt tacgattatt gttccagcgg tgcagacgat ccagagatgc
    13561 ttcaacatca gactcgctgg catcgcgcaa tgcgccctgt tcggcgatag actcttgcag
    13621 atgcagacct gccgcacgac caaagaccac caggtccagc agcgagttgc cgcccagacg
    13681 gttagcgccg tgtaccgata cacaagcgat ttcaccaacg gcaaacagcc ccggaacaac
    13741 cacatcttcg cctttctcgt tcaccgtcag cgcctgaccg gtcactttgg tcggaatacc
    13801 gcccatcatg tagtgacagg ttgggataac cggaatcggc tctttcaccg gatcgacgtg
    13861 agcaaaggta cgggaaagct ccaggatacc cggcagacgg gattcgagaa cttctttacc
    13921 caggtgatcg agtttcagtt tcgcgtgtgg cccccacgga ccatcacagc cgcgaccttc
    13981 acggatttca atcatgatgg aacgcgccac cacatcacgg cccgccaggt ctttggcgtt
    14041 cggcgcataa cgctccataa agcgttcgcc atgtttgttc agcagataac cgccttcacc
    14101 acggcaacct tcggtgacca gtacgcccgc accggcaatg ccggtcgggt ggaattgcca
    14161 catttccata tcctgcaccg gtacgcctgc gcggatagcc atgccgacac cgtcgccagt
    14221 gttaatgtgg gcgttagtgg tggactgata aatacgccct gccccgccag tcgccagcac
    14281 ggtagcgcgg gctttgaaat aaaccacttc accggtttcg atgcacagtg cggtacaacc
    14341 caccaccgcg ccatcctggt ttttcaccag atccagcgca taccactcgg agaaaatggt
    14401 ggtgtggttt ttcaggttct gctgataaag cgtgtgcaac agtgcgtgac cggtacggtc
    14461 agccgccgcc gcagtgcgtg ccgcctgctc gccgccgaag tttttcgact gaccgccaaa
    14521 cggacgttga tagatacgac catcatcaag acgcgagaac ggcaggccca tgtgttcgag
    14581 ttccagaatc gcttccggcc cggttttaca catatattca atcgcgtcct ggtcaccgat
    14641 atagtccgac ccttttacgg tgtcgtacat atgccattcc cagttatctt catgggtatt
    14701 acccagcgca acggtaatgc cgccttgcgc agaaacggta tgggaacggg tcgggaagac
    14761 tttagagagc agcgcacagg tctggccgct ctgggaaatt tgcagcgccg cgcgcatacc
    14821 tgcgccaccg gcaccaatca caactgcatc aaattctctg actggcaatt tcatcacaca
    14881 ccccacacca caacgaatcc ataaatcacg taaaccacca gtgcaacgac aatcaccagt
    14941 tgcagcatca ggcgcaatgc cagcggttta acgtagtcgg tcaacacctg ccacatgccg
    15001 atccaggcat gaatcaagat agaaaacagc gccagcaggg tgaacacttt ggtgaacgca
    15061 gaggcgaaga aaccgatcca gacttcatat gtcagttcgc cactggtggc gaaaaaaccg
    15121 accatataaa tgatgtagag cgtcaggacg atagcggtag cgcgaacgag gatgaaatca
    15181 tgtacgccat tgcgtcctaa tgcggaggcg ttgcttacca tacgaggact cctgcgagaa
    15241 gtgaaagcac gacagtaata acaaaggaga ttttggcgga gcgtttaccc gcttcgaatg
    15301 tttcttccag atagccaaaa tccatcatca tgtggcgaat acctacgacg acgtgatacg
    15361 ccagagcggt aaggatgccc cacatgataa atttgacgaa gaagctgccc ataatcgcgg
    15421 aagcttgctc gaaaccttca ggggaagaga ggctggtacc cagaagccac agcaggatgc
    15481 ccactgcaac aaaggtgatc acaccggaaa cgcgatggag aatggacgct atcgccgtga
    15541 tggggaaccg gatggtctgt aggtccagat taacaggtct ttgttttttc acatttctta
    15601 tcatgaataa cgcccacatg ctgttcttat tattccctgg ggactacggg cacagaggtt
    15661 aactttctgt tacctggaga cgtcgggatt tccttcctcc ggtctgcttg cgggtcagac
    15721 agcgtccttt ctataactgc gcgtcatgca aaacactgct tccagatgcg aaaacgacac
    15781 gttacaacgc tgggtggctc gggattgcag ggtgttccgg agacctggcg gcagtatagg
    15841 ctgttcacaa aatcattaca attaacctac atatagtttg tcgggtttta tcctgaacag
    15901 tgatccaggt cacgataaca acatttattt aatttttaat catctaattt gacaatcatt
    15961 caacaaagtt gttacaaaca ttgccaggaa aagcatataa tgcgtaaaag ttatgaagtc
    16021 ggtatttcac ctaagattaa cttatgtaac agtgtggaag tattgaccaa ttcattcggg
    16081 acagttatta gtggtagaca agtttaataa ttcggattgc taagtacttg attcgccatt
    16141 tattcgtcat caatggatcc tttacctgca agcgcccaga gctctgtacc caggttttcc
    16201 cctctttcac agagcggcga gccaaataac aaacgggtaa agccaggttg atgtgcgaag
    16261 gcaaatttaa gttccggcag tcttacgcaa taaggcgcta aggagacctt aaatggctga
    16321 tacaaaagca aaactcaccc tcaacgggga cacagctgtt gaactggatg tgctgaaagg
    16381 cacgctgggt caagatgtta ttgatatccg tactctcggt tcaaaaggtg tgttcacctt
    16441 tgacccaggc ttcacttcaa ccgcatcctg cgaatctaaa attactttta ttgatggtga
    16501 tgaaggtatt ttgctgcacc gcggtttccc gatcgatcag ctggcgaccg attctaacta
    16561 cctggaagtt tgttacatcc tgctgaatgg tgaaaaaccg actcaggaac agtatgacga
    16621 atttaaaact acggtgaccc gtcataccat gatccacgag cagattaccc gtctgttcca
    16681 tgctttccgt cgcgactcgc atccaatggc agtcatgtgt ggtattaccg gcgcgctggc
    16741 ggcgttctat cacgactcgc tggatgttaa caatcctcgt caccgtgaaa ttgccgcgtt
    16801 ccgcctgctg tcgaaaatgc cgaccatggc cgcgatgtgt tacaagtatt ccattggtca
    16861 gccatttgtt tatccgcgca acgatctctc ctatgccggt aacttcctga atatgatgtt
    16921 ctccacgccg tgcgaaccgt atgaagttaa tccgattctg gaacgtgcta tggaccgtat
    16981 tctgatcctg cacgctgacc atgaacagaa cgcctctacc tccaccgtgc gtaccgctgg
    17041 ctcttcgggt gcgaacccgt ttgcctgtat cgcagcaggt attgcttcac tgtggggacc
    17101 tgcgcacggt ggtgctaacg aagcggcgct gaaaatgctg gaagaaatta gctccgttaa
    17161 acacattccg gaatttgttc gtcgtgcgaa agataaaaat gattctttcc gcctgatggg
    17221 cttcggtcac cgcgtgtaca aaaattacga cccgcgcgcc accgtaatgc gtgaaacctg
    17281 ccatgaagtt ctgaaagagc tgggcaccaa agatgacctg ctggaagtgg ctatggagct
    17341 ggaaaacatc gcgctgaacg acccgtactt tatcgagaag aaactgtacc cgaacgtcga
    17401 tttctactct ggtatcatcc tgaaagcgat gggtattccg tcttccatgt tcaccgtcat
    17461 tttcgcaatg gcacgtaccg ttggctggat cgcccactgg agcgaaatgc acagtgacgg
    17521 tatgaagatt gcccgtccgc gtcagctgta tacaggatat gaaaaacgcg actttaaaag
    17581 cgatatcaag cgttaatggt tgattgctaa gttgtaaata ttttaacccg ccgttcaaat
    17641 ggcgggttaa tttttatatg cccaaacaca aaaaattgta aaaataaaat ccactatcgg
    17701 acattcatat attctaaaaa aaatatatcg gtataaatta taaacaaatc gatgattttt
    17761 aatcatctat cttcctctca gttacatgaa taaaccacca aatatagcgc acccctataa
    17821 tttccaatac tttattcatc aaacatttaa ttcataccat caattaatta cttttacatt
    17881 taataaaatt aataatattt ttaagaaaaa ctaaaaacat tcgacaatca aataagatat
    17941 tcttgcaccg atatactttt ttcttttctg ttaaaggact cagttatgtt taaaggacaa
    18001 aaaacattgg ccgcactggc cgtatctctg ctgttcacag cacctgttta tgcggctgat
    18061 gaaggatccg gtgaaattca ctttaaaggt gaagttattg aagcaccgtg tgaaatacat
    18121 caggatgata ttgataaaga ggttgaactc ggtcaggtga ccaccagcca cattaatcag
    18181 tcacatcaca gcgatgccgt tgctgtcgac ctgcgcttag tcaactgtga tctggaaaac
    18241 tccagcaacg gttccggtgg caagatttct aaagttgcag tcacctttga tagctcagcg
    18301 aaaaccaccg gcgcagatcc gattctcaac aacaccagca cgggtgaagc caccggcgtc
    18361 ggcgtacgtt taatgaataa agatcaaagc aatatcgttt taggcaccgc tactccagat
    18421 atcgacctgg ctccgacctc cagcgaacaa acgctgaatt tctttgcctg gatggaacaa
    18481 attgatcagg ctacacctgt aacgccaggc gcagttacgg cgaatgcaac ctacgtgctg
    18541 gattataaat aagtattact ttggcggcgc tatcgccgcc tttcataaaa tatttatcag
    18601 gtatggacac cgtgaatatt tatcgactct cttttgtatc ctgcctggtc gtggcgatgc
    18661 cttgcgcatt ggcggtcgaa ttcaacctta atgttctcga taaatcgatg cgcgaccgca
    18721 ttgatatttc attattaaag gaaaaaggag tcattgctcc cggtgagtat tttgttagcg
    18781 ttgcggtaaa taataaccaa atcagtaacg ggcaaaagat taactggcac aaaaatgacg
    18841 ataaaaccat tccgtgcatc aatgatttac tggtcgataa atttggctta aaacctgaag
    18901 tccgtcagtc gttaccattg ataaatcagt gcgtcgattt tagctcccga cctgaaatgc
    18961 tcttcaattt cgatcaagcc aatcagcaac tgaatattag tattccgcaa gcctggctgg
    19021 cgtggcactc agaaaactgg accccaccct ccacatggaa agaaggtgtc gccggtatcc
    19081 tgatggatta caacttgttt gccagcagct accgcccaca ggacggcagc agcagcacta
    19141 acctgaacgc ctacggtacc accggaatta acgccggggc atggcgctta cgtagtgatt
    19201 atcagttgaa tcagactgat agcgatgata accatgaaca gtcaggcgga atatcgcgca
    19261 cctatctttt tcgtccatta ccgcaattag gctctaaatt aaccctcggc gaaacggatt
    19321 ttagttccaa tattttcgac ggtttttctt ataccggcgc ggcactggca agtgacgagc
    19381 gaatgttgcc atgggaacta cgcggctacg ccccacaaat tagcggtatt gcacagacca
    19441 atgccacggt gacgatcagt caatcaggcc gcgtcattta ccagaaaaaa gtcccaccag
    19501 gcccatttat cattgacgac cttaatcagt ctgttcaggg cacactggat gtcaaagtga
    19561 cggaagaaga tggtcgggtg aacaatttcc aggtttcggc agcatcgacg cccttcctga
    19621 ctcgtcaggg acaggttcgc tataaactgg ccgcgggtca gccacggccc tccatgtcac
    19681 atcaaactga aaatgaaacc ttttttagca atgaagtttc ctgggggatg ctgtcaaaca
    19741 cctcgctgta cggcggcctg ctgctttctg gtgatgacta ccattctgcc gcaatgggta
    19801 ttgggcaaaa tatgctgtgg cttggtgcgc tgtcgtttga tgtcacgtgg gccagtagcc
    19861 attttgatac tcagcaggac gagcggggct taagctaccg ttttaattac agcaaacaag
    19921 tggatgccac taacagcacg atttccctcg ccgcttatcg tttctccgat cgtcattttc
    19981 acagctacgc caactatctg gatcacaaat acaacgacag cgatgcgcag gacgaaaaac
    20041 agacgatcag cttattgtgg gccaaccgat taccccacta aacctcaatc tttacgccaa
    20101 cctgctacat caaacctggt ggaatgcaga cgcctccacg accgccaaca tcacggcagg
    20161 ttttaatgtt gatattggtg actggagaga tatctctatt tcgacgtcat tcaatacaac
    20221 ccattacgaa gataaagatc acgacaacca gatttacctg tcgatttcgc tccccttcgg
    20281 taacggtggt cgggttggct atgacatgca aaacagtagc cacagcacca cacaccgcat
    20341 gtcgtggaac gatacgctgg atgaacgtaa tagctggggc atgtctgccg gactgcaatc
    20401 cgaccgtcct gacaatggag cccaggtgag cggtaactat cagcacctga gttcagcggg
    20461 tgagtgggat atttctggta cctatgccgc caatgattac agttccgtca gcagcagctg
    20521 gagcggttct ttcaccgcaa cccaatatgg tgcagcgttt catcgccgca gctccaccaa
    20581 tgaaccacgc ctgatggtca gcaccgatgg cgtggcagat attccggttc agggcaatct
    20641 cgactacacc aaccattttg gcattgcggt ggtgccgttg atttccagtt accagccttc
    20701 caccgtggcg gtgaacatga atgacttacc cgacggcgta acagttgcag aaaacgttat
    20761 caaagaaacg tggattgaag gcgcgatagg ttacaaatca ctggcttccc gttccggtaa
    20821 agacgttaac gtcatcattc gcaacgccag cggtcagttt cctcccctcg gagcggatat
    20881 ccgccaggat gacagcggca ttagcgtggg gatggttggc gaggaaggac atgcctggtt
    20941 aagcggagtc gctgaaaatc aaaagtttac cgtggtctgg ggtgatagcc agcattgctc
    21001 gctccatctt cctgaacata tggaagacac cgcaaatcgc ctgattttac cttgtcatta
    21061 attaaggaaa tgaaaatgac atttatgaaa ggactacccc ttttgttgtt ggttgccagt
    21121 ctgtgcagcc acgctgcact acaacccgat cgcactcgta ttgtgtttaa cgctaatgat
    21181 aaagctacca gtctgcgggt ggataatcgt agcgataaac tcccctacct cgcttattcc
    21241 tggctcgaaa atgaaaaagg ggagaaaagc gatgatcttc tggtcgccct gccgccgata
    21301 caacgtctgg aaccgaaagc aacaacgcag gtacggatag taaagcaagc ctcgaccaca
    21361 aaattaccgg gcgatcgcga aacgctattt ttctacaata tgcgtgaaat tcctcccgcc
    21421 ccggaaaaaa acagcgacca tgccgttctg caagtcgcca ttcagagtcg tatcaaggtg
    21481 ttctggcgac cggctgcatt acgcaagaaa gcaggagaaa aggtcgaact gcagttacag
    21541 gtcagccagc agggcaacca actgacactg aaaaatccta ccgcgtatta tctgaccatt
    21601 gcctacctgg gacgaaatga aaaaggcgtt ctccccggct tcaaaaccgt gatggttgcg
    21661 ccttttagta cggtcaacac aaacaccgga aattatagcg gcagccagtt ttatctcggt
    21721 tatatggatg attacggcgc gttacgcatg acgacgctca actgcagcgg acaatgccat
    21781 ttacaagccg tggaggcgaa gaaatgagtg ctggcaaggg agtgttgctc gtcatctgtc
    21841 tgttatttct gccgttgaag tctgcgcttg ccctgaactg ctactttggt tcatccggtg
    21901 gctcggtaga aaaatcagaa gcgattcaac cttttgctgt accaggcaat gccaaactcg
    21961 gcgataagat ttgggaatct gacgatatta aaattcccgt ctattgtgac aacaatacca
    22021 acggcaattt tgaaagtgag catgtttatg cctgggtaaa cccctacccg ggggtgcagg
    22081 atcgctatta tcaactcggc gtaacttaca atggcgttga ttatgacgcc aaccagggaa
    22141 aaagccgcat cgacactaac cagtgtattg acagtaagaa cattgacatt tatacccctg
    22201 agcagattat cgcgatggga tggcagaaca aaatttgctc gggtgatccc gctaatattc
    22261 acatgtcgcg tacctttctc gctcgcatgc ggttatacgt caaaatacga gaaatgccgc
    22321 cgcatgatta tcaaagtacg cttagcgact atatcgtcgt gcaatttgac ggtgccggta
    22381 gcgttaacga agaccctact gcccaaaacc tgaaatatca tattactggt ctggaaaaca
    22441 ttcgcgtgct ggattgcagc gtcaattttt ccatttcacc ggaaacacag gtgattgatt
    22501 ttggtaaatt taatttgctg gatattcgtc gccacacaat gtcgaaaaca ttcagtatta
    22561 agacgacaaa atcacaaaat gaccaatgca ccgacggatt taaagtcagt tcctcctttt
    22621 ataccgaaga aacgttggtt gaagaagata aagcattgtt gattggtaat ggtctgaagc
    22681 tacgtttgct ggatgaaaac gcctcgcctt ataccttcaa taaatatgct gaatacgccg
    22741 atttcaccag cgacatgttg gtctacgaaa aaacctatac agctgaactt tcgtccattg
    22801 caggtacccc catcgaggct gggcccttcg atacagtggt gctttttaaa attaactata
    22861 actgatttgc catgatgccc tttgcagccg gaaagggcat ctctttcaga aaattgctga
    22921 ttttcccctt ttccttgatt gctgttattt tttcctggcc cggctttctt tatgctgtat
    22981 cgatacacca aagagaaaat aatgagggag cgaaggatgc cagttttgca gtggggaatg
    23041 ttatgtgtgc tatcacttct cctttctatt ggtttcctcg cggtacatct cccggcggcg
    23101 ctattacttg ggccgatgat cgccgggatc atcttcagta tgcgcggaat tactctgcaa
    23161 cttccccgct ccgcttttct cgccgcgcag gccattcttg gctgcatgat tgcgcaaaac
    23221 ctcactggtt cgattctcac cacgctggcc gccaactggc cgatcgtgct tgctatttta
    23281 ctggtgacgc tgctttccag cgccatcgtg ggttggttat tggtgcgcta tagctcactg
    23341 cctggaaata ccggtgcctg gggttcttct ccaggcggcg cagcggcaat ggtcgctatg
    23401 gcgcaagatt acggcgcaga tattcgcctg gttgcattta tgcaatatct gcgagtgctg
    23461 ttcgtcgcgg gggccgctgt tctggtcacc cgcatgatgc tgggtgataa cgctgaagcg
    23521 gttaaccagc aaatcgtctg gttcccgcca gtgagcatta atctcctgct tacgattttg
    23581 ctcgcagtcg ttgccggtac ggcgggatgc atgctgcgtc ttccttccgg cacgatgctc
    23641 atcccgatgc tggcgggcgc agtgctccag tctggtcagc tcatcaccat cgaactaccg
    23701 gaatggctgc tggcgatggc gtatatggca attggctggc ggattggtct tggtttcgat
    23761 aaacaaatct tactgcgggc actacgcccg ctaccgcaaa tcctgctgtc gatttttgct
    23821 ctgctggcta tttgtgcggg tatggcgtgg gggctgaccc ggtttatgca cattgatttt
    23881 atgactgcct acctcgccac cagtcccggc gggcttgata ccgtggcggt catcgccgca
    23941 gggagcaatg ccgatatggc gcttattatg gcgatgcaaa ccctgcgcct gttcagtatt
    24001 ttgctgacgg ggccagccat tgcgcggttt atttcaacct atgcgccgaa gcggtcggcc
    24061 tagtgctggc agccagggca ccagtaaaac gggcgagatg acagcgtggt tttctcaatg
    24121 atgctgccac agcgttcgca cggttcgcca tcacgatgaa aaaccttaaa gcgaaacagc
    24181 gccccatgat gcttattctc atccaccagc cccctcgtag cgtaggaaaa tcgaggaata
    24241 tccagtaacg cgtgtgcgag tgcatccagt tgcgccgcat tgagatcttt cgctttatga
    24301 tttccagtca acccaacctg ccagaggatc tccacccgca aataattgcc aagcccagcc
    24361 agaaacgcct gatcgagcag taatcctgca aactgacggt tacgaaagcg cggtgacaat
    24421 aaccgttcct ttaccacctc cggcgtcaga ttcggatcca gcacatcggg accaacgcgt
    24481 tgtaaaaacg gatgcgtggt cagttgttcc ggcctcaaca tctcaatatc cgaggcgcta
    24541 taaagcagaa tggttttgtc agccgtttgc agttttaccc gcaatactcg cgtggtctgc
    24601 ggctcttcgc cggtatcaac cacgcgccag acgccgtaaa gctgattatg gctgtagagc
    24661 gttaagtcgt tggaaaaatg agttaacaac gccttaccac gcgtttccac atgggtaacg
    24721 tgttgaccga taagttgtga ttgataagtt tttaactgcg ggaaggcaaa ccagacatca
    24781 gttagtggtt tgcctttgat cgccgcctcc aggttatccg ctgcacggcg gatctccggg
    24841 ccttcaggca tggtgatatc cttttttcat tctttattaa tcggggttat gctgcgacaa
    24901 caatctcctt ttcggcaaat gtagagcgta gtcggcgggc gaacgccagt gcgtgctcgc
    24961 cgtcgccatg caggcagact gtttgcgccg tgaccgttgc ccattcgccg gtgatacttt
    25021 tgactctgcc gtgttgcacc atctctagcg tttgcgccaa cgcctgttct tcgttttcaa
    25081 tcaacgcgcc tcgctgactt cgcggcacca gcgagccgtc agcctgataa ccgcgatcgg
    25141 caaacacttc ctcgcgcgtt gtcaggccat attgcttgcc tgcacgaatc agctcgcttc
    25201 ctgccagccc aacgagaatc aatgccggat cgcaagcgta taccgctctg gcgatggcgt
    25261 ctgccagttg tgcttctttc gccgcctggt tgtacaacat gccgtgcggt ttgacatgac
    25321 gcattacgcc gccttgcgca cgggcaatcg ttgccagcgc gccaatttga tacagcgtct
    25381 gggcgtaaac ggtttctggc ggcagctgca tggcgctgcg accaaaattt tccctgtcgg
    25441 gaaaactcgg gtgagcgcca atcgcgacac cattttttat tgcttcacgc acgcaagcct
    25501 gcatgatttg cgcatcgcct gcatgaaatc cacaggcaat attggcagag gaaaccagcg
    25561 ttaatagctc tgcgtcgctg gcgcagcctt cgcccagatc ggcgttcagg tcaattttca
    25621 ttgtgcagcc gccacactaa ttgttcgaaa taacgttgct gatcttgccg cgctttcagt
    25681 gcctcttcca gtgaacactg gacaaaatga atcggctgac cgagcggaat ttgcgccaga
    25741 tggtacatat cagcctcaat gatacaggca atacgcgggt aaccatcggt ggtctgtgcg
    25801 tcgttcatca acacaatcgg ctgcccgtta tgtggcacct gcaccacgcc cggtaacaaa
    25861 ccgtgagata acagttcgcg atcggtggtg cgttttaaaa tttgcccctg taagcgatag
    25921 cccatgcggt tactttgcga gctaagctgc cagggcgaac gccagaatgc atcctgcgag
    25981 gcgcgatcga actcatgata ttccggcccc ggcaaggcgc gaatgcggtt gccccacagc
    26041 agctgtttaa cgccctgcgc ttccataaaa tcacgcttcg atttgccaat cgggagtcgg
    26101 tcaccatcct tcagtaaacg gccttccagc ccgccaatcc ccactttgag atcggtgctg
    26161 catgacccca ttaccggcgg aacatcaata ccacccgcga ccgccagata actgcgcatc
    26221 ccgtgctgcg ggcgtttaag cgttaaacgc tggcctgctt tcatcggcaa tcgccagccg
    26281 gtccagacgg cattatcatc cagccgcgct tcgcaaccgg cacccgtcag agcaaaccac
    26341 ccatcagttt cgaactcaac cgttaactga ccgagcgtga tctccagcgc gggggcattg
    26401 gcgtcattac ccaccagcag gttagcaatg cgtaacgcag gcatatccag tgcgccgcag
    26461 tggctgattc ccgactggcg aaaaccgtga cgaccgccat cctgcacagt ggtatacatg
    26521 cccgcacgaa taatcttcag catactccct ccttctgcgg tacaaagcgc acgctgtctc
    26581 ccggacgtaa taagatgggt tcgtcacgcg ccggatcaaa caggctgagt gaggtatgac
    26641 caatcaactg ccagccaccc ggcgttgcca gcggataaac accagtctgc ggcccgccga
    26701 tcccgacaga acctgccgga acgagcaagc gcggttcagc gcgccgtggc gtgtgtagtt
    26761 gttccggcaa actcccgaga tacgggaagc ccggttgaaa acctaaaaac cagaccacgt
    26821 attccacgga ggagtgcaat tcaacaacct gtttttcgct aaacccgcaa tgcgccgcga
    26881 ccaccgccaa atccggtccg cctgcaccac cgtaaaccac cggaatttca ataaagcgag
    26941 actcaggttc cagggcctcg ctctcctccc accagcgttg caaacgctct atggcatcca
    27001 gcgccagcga ctcaggatta cgcaaaatca ccgtgatatt gttcatgccg ggaatggctt
    27061 caaccacatt cggcatatcc accagacgct gcgccagtcg ccagatccgt ttctggctag
    27121 ccagcgtcac cggcggttcc agttccagca ctaccgccgt ttcacctatc agataacaac
    27181 gcgctcgttg cactttcgat ccctctgatt attcgttatg caggattagg aatatcaata
    27241 aaggtcacat caagatcggt attttcattc agccactcgc tcaatgcgcg aataccacca
    27301 cgttcagtgg catggtgacc tgcagcataa aaatgcaacc cctgctcgcg ggctgaatga
    27361 atggtctgtt cagaaacttc gccagtaata aaagcatcca cgccaaaacg cgcggcgcta
    27421 tcgataaaac tttgcccgcc gcccgtgcac caggcgacgc gctggaccac ctcaggtccg
    27481 gtatcgccac accataacgg cttacgtccc agacgcgctt caatccagga ggctagttcc
    27541 agtcccggca caggcatggt cagctctccc cacggcacca acggctcaat ttcgcccatg
    27601 accgtgatcc ccagtaacgc cgccagctgt gcgttattgc ccagctcagg atgtacgtca
    27661 agcggcaaat gccagccata caggttgata tcattcgcca gcaacgtttt taaacggtta
    27721 cgcttcattc cgcgaatgac cggagactct cctttccaga agtagccatg atgcacaatg
    27781 accgcatccg cgcccagacg cactgcctca tcgagcaaag cctggctggc ggtgacaccg
    27841 gtaacaattt tttgcaccgt ctctttgcct tctacctgca aaccattcgg cgcgtaatca
    27901 ctaatcgccg cgctgttcag tttttcgtta atcagttgtt ccagttcggt gtttttcatc
    27961 ttcgcgcctt ccgtattctg atagctacac cgttaacata gcgttacctc gcctgttcgt
    28021 cgatagccaa tcttttgaca tattaagaaa caaacccgac gccaaaagac gcaaattgtt
    28081 tatatcatca cgcttcagct aatgtatatt tatgcaatca gacagcatat tgcataaata
    28141 atcagatctg tatcaaagtt ttaactttaa ggaaacacaa gcacgccgcg caactattac
    28201 gtgacgttgc agtcaattgt ggaattatta tgaataaaca cgcatcacaa ccgcgcacta
    28261 tttactatgt cgttgcgctg caaatctggg aatattttag cttttacggc atgcgtgccc
    28321 tgctgattct ctatctcacc aatcaactaa aatacaacga tactcacgcc tacgagttat
    28381 ttagcgccta ctgttcgctg gtgtatgtca cgccaatcct cggtggcttt ttggcggata
    28441 aagttctcgg caatcgcatg gcggtgatgc tgggggcgtt gttgatggcg atcggtcatg
    28501 tggtgctggg tgccagtgag atccatccgt cattcctcta tctgtccctg gcgattatcg
    28561 tttgtggcta tggtctgttt aaatccaatg tcagttgcct gctcggcgag ctgtatgagc
    28621 caaccgatcc gcgtcgtgat ggcggtttct cgctgatgta tgcagcgggt aacgtggggt
    28681 ctattatcgc acctatcgcc tgtggttacg cccaggaaga gtacagctgg gcgatgggct
    28741 ttggcctggc ggcggtgggt atgatcgcgg gtctggtcat tttcttatgt ggcaatcgtc
    28801 atttcacaca tacccgcggc gttaataaaa aagtactgcg tgcaacaaac tttctcctgc
    28861 ctaactgggg atggctgctg gttctgctgg tggcaacgcc tgcgctgatt accgtactgt
    28921 tctggaaaga gtggtcggta tacgccttaa ttgtcgcgac tattattggc ctgggtgtac
    28981 tggcaaaaat ttatcgcaaa gcagaaaacc agaaacagcg taaggagctg gggctgattg
    29041 tgacgctcac cttcttcagt atgttgttct gggccttcgc acaacagggc ggcagctcaa
    29101 ttagccttta tatcgaccgc ttcgttaacc gcgatatgtt tggttatacc gttccgactg
    29161 ctatgttcca gtcgattaat gccttcgcag ttatgctatg cggtgtgttc ctggcgtggg
    29221 tggttaaaga aagcgtcgca ggtaatcgta ccgtgcgcat ctggggaaaa tttgctcttg
    29281 gccttggcct gatgagcgcc ggattctgca ttctgacctt aagcgcccgc tggtccgcaa
    29341 tgtatggtca ctcttctctg ccactgatgg tgttaggcct cgcggtgatg ggctttgcgg
    29401 aactgtttat cgacccggtt gccatgtcgc aaattacgcg cattgaaatt ccgggcgtga
    29461 ccggcgtatt aaccggcatc tacatgctgc tttctggcgc gatcgcgaac tatctggcgg
    29521 gcgtgattgc cgatcagaca tcgcaggctt cgtttgatgc ttccggggcg atcaactact
    29581 ccatcaatgc atatattgaa gtatttgatc aaattacctg gggcgcactg gcgtgtgtag
    29641 gactggtact gatgatttgg ctgtatcagg cgctgaaatt cagaaaccgc gcgctggcgc
    29701 tggagtcata ataggccagc accgatgcct gatgcgacgc ttgacgcgtc ttatcag
//
LOCUS       DAZPAC010000031        29548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZPAC010000031 DAZPAC010000000
VERSION     DAZPAC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.031"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="ACHUPM_001131"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="ACHUPM_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HFR0868974.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="ACHUPM_001132"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="ACHUPM_001132"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR0868975.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHUPM_001133"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHUPM_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFR0868976.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="ACHUPM_001134"
     CDS             complement(3644..4030)
                     /locus_tag="ACHUPM_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR0868977.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHUPM_001135"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHUPM_001135"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFR0868978.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHUPM_001136"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHUPM_001136"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR0868979.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHUPM_001137"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHUPM_001137"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR0868980.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHUPM_001138"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHUPM_001138"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR0868981.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHUPM_001139"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHUPM_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR0868982.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="ACHUPM_001140"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="ACHUPM_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR0868983.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHUPM_001141"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHUPM_001141"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR0868984.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="ACHUPM_001142"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="ACHUPM_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR0868985.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="ACHUPM_001143"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="ACHUPM_001143"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR0868986.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="ACHUPM_001144"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="ACHUPM_001144"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR0868987.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHUPM_001145"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHUPM_001145"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR0868988.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="ACHUPM_001146"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="ACHUPM_001146"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR0868989.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="ACHUPM_001147"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="ACHUPM_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR0868990.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="ACHUPM_001148"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="ACHUPM_001148"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR0868991.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHUPM_001149"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHUPM_001149"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR0868992.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHUPM_001150"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHUPM_001150"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR0868993.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHUPM_001151"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHUPM_001151"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR0868994.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHUPM_001152"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHUPM_001152"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR0868995.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHUPM_001153"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHUPM_001153"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR0868996.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHUPM_001154"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHUPM_001154"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR0868997.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="ACHUPM_001155"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="ACHUPM_001155"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR0868998.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7120 c         8160 g         7109 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgataaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcctgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttctcgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAZPAC010000032        29419 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZPAC010000032 DAZPAC010000000
VERSION     DAZPAC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29419
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.032"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..642)
                     /locus_tag="ACHUPM_001156"
     CDS             complement(<1..642)
                     /locus_tag="ACHUPM_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR0868999.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
     gene            complement(742..1653)
                     /gene="gsiD"
                     /locus_tag="ACHUPM_001157"
     CDS             complement(742..1653)
                     /gene="gsiD"
                     /locus_tag="ACHUPM_001157"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR0869000.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1656..2576)
                     /gene="gsiC"
                     /locus_tag="ACHUPM_001158"
     CDS             complement(1656..2576)
                     /gene="gsiC"
                     /locus_tag="ACHUPM_001158"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR0869001.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2594..4132)
                     /gene="gsiB"
                     /locus_tag="ACHUPM_001159"
     CDS             complement(2594..4132)
                     /gene="gsiB"
                     /locus_tag="ACHUPM_001159"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR0869002.1"
                     /translation="MAKAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4152..6023)
                     /gene="gsiA"
                     /locus_tag="ACHUPM_001160"
     CDS             complement(4152..6023)
                     /gene="gsiA"
                     /locus_tag="ACHUPM_001160"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR0869003.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6010..6975)
                     /gene="iaaA"
                     /locus_tag="ACHUPM_001161"
     CDS             complement(6010..6975)
                     /gene="iaaA"
                     /locus_tag="ACHUPM_001161"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR0869004.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7179..8414
                     /gene="moeA"
                     /locus_tag="ACHUPM_001162"
     CDS             7179..8414
                     /gene="moeA"
                     /locus_tag="ACHUPM_001162"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR0869005.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8414..9163
                     /gene="moeB"
                     /locus_tag="ACHUPM_001163"
     CDS             8414..9163
                     /gene="moeB"
                     /locus_tag="ACHUPM_001163"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR0869006.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9342..10004)
                     /gene="fsa"
                     /locus_tag="ACHUPM_001164"
     CDS             complement(9342..10004)
                     /gene="fsa"
                     /locus_tag="ACHUPM_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR0869007.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10135..11034
                     /locus_tag="ACHUPM_001165"
     CDS             10135..11034
                     /locus_tag="ACHUPM_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR0869008.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11040..13472
                     /locus_tag="ACHUPM_001166"
     CDS             11040..13472
                     /locus_tag="ACHUPM_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR0869009.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13618..14433
                     /gene="ybiV"
                     /locus_tag="ACHUPM_001167"
     CDS             13618..14433
                     /gene="ybiV"
                     /locus_tag="ACHUPM_001167"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR0869010.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14585..15850
                     /locus_tag="ACHUPM_001168"
     CDS             14585..15850
                     /locus_tag="ACHUPM_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR0869011.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15817..16977)
                     /locus_tag="ACHUPM_001169"
     CDS             complement(15817..16977)
                     /locus_tag="ACHUPM_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0869012.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17215..18516
                     /locus_tag="ACHUPM_001170"
     CDS             17215..18516
                     /locus_tag="ACHUPM_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0869013.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18531..20894
                     /locus_tag="ACHUPM_001171"
     CDS             18531..20894
                     /locus_tag="ACHUPM_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HFR0869014.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21074..22669)
                     /locus_tag="ACHUPM_001172"
     CDS             complement(21074..22669)
                     /locus_tag="ACHUPM_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR0869015.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22888..23808
                     /gene="ldtB"
                     /locus_tag="ACHUPM_001173"
     CDS             22888..23808
                     /gene="ldtB"
                     /locus_tag="ACHUPM_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0869016.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23867..24985)
                     /locus_tag="ACHUPM_001174"
     CDS             complement(23867..24985)
                     /locus_tag="ACHUPM_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR0869017.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24982..25449)
                     /gene="mntR"
                     /locus_tag="ACHUPM_001175"
     CDS             complement(24982..25449)
                     /gene="mntR"
                     /locus_tag="ACHUPM_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR0869018.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25635..25763
                     /gene="mntS"
                     /locus_tag="ACHUPM_001176"
     CDS             25635..25763
                     /gene="mntS"
                     /locus_tag="ACHUPM_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR0869019.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26035..27618
                     /gene="opgE"
                     /locus_tag="ACHUPM_001177"
     CDS             26035..27618
                     /gene="opgE"
                     /locus_tag="ACHUPM_001177"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR0869020.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27667..28182)
                     /gene="ompX"
                     /locus_tag="ACHUPM_001178"
     CDS             complement(27667..28182)
                     /gene="ompX"
                     /locus_tag="ACHUPM_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR0869021.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28235..28300)
                     /locus_tag="ACHUPM_001179"
     CDS             complement(28235..28300)
                     /locus_tag="ACHUPM_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869022.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28535..>29419
                     /gene="rhtA"
                     /locus_tag="ACHUPM_001180"
     CDS             28535..>29419
                     /gene="rhtA"
                     /locus_tag="ACHUPM_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR0869023.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         7125 a         8063 c         7384 g         6847 t
ORIGIN      
        1 caaatcgtga tctttgccgt tgatacgtag cgtaaaagag atacattttt tacccggacg
       61 atcaaagcaa ttgtatgccg gattccagct gttgttagag tcatctgaag ccaccgacca
      121 cggtcgtttc gatgggttaa aatcggcaaa aataacccgc cctgactcgt cgacaagcgg
      181 tccatttact tgttcctgat gataaacaac gattttatca ttcgccctcc gcgaaaagag
      241 ataatattta ttatcatcaa gtaccagtcc caccgagtct aaaaatggca tcatttccat
      301 tttcagaatc acggcgcgtt gaatctcctc tgtatttttt aataaataca gtgagtcctt
      361 taatgtcagg gccgaacgtt caatatcttt aaagaaatag ccaacgtttt tctgaataag
      421 ttcaaagcgg ctatcggcac gttttaccgc gcgttccgat accagttgat agttaatgat
      481 gaaaaaaaga ccaataaatg acagtgctgc caataaaaat aaaattatca gccttatctt
      541 tatcttttcg tataaactca gcatgttaat acatttaaca aaataactat ctgaacgctg
      601 tgccgctgca aaaatagtcc cttgctgcgg ctttttaacc aaattacccg attctacaaa
      661 atcagcagaa agttacaaca cttgcttagg gcaataattt cctgtgaggt gattaccctt
      721 tcaagcaata ttcaaacgta actatccttt aattttcgga tccagcgcat cgcgtaaacc
      781 atcgcccaac aaattgaacg ccagtacggt cagaaaaata gccagggccg gaaaaacagc
      841 gacatgcggc gcgataacca tatccgctcg tgcctcattg agcattgctc cccactctgg
      901 tgtcggcggc tgcgcaccga ggccgagaaa tgacaggctg gcggcagaga taatcgaggt
      961 gccaatgcgc atggtgaaaa acaccacgat agacgagacg gtcccaggca agatatgacg
     1021 caataaaatg gtcatatcgc tggcaccaat actgcgtgct gactcaataa aggtttgctg
     1081 tttcaacacc agcgtgttgc cgcgcaccag tcgggcaaac gcggggatgg aaaaaatggc
     1141 gactgcgata atcacgttag caatgccgct tcccaacacc gcaacaacag cgatcgccag
     1201 taaaataccc gggaaggcaa acagcacatc gcaaatgcgc atgatcagcc gatcccacca
     1261 gccttcataa tatccagcca acaagcccag caacgtcccg atcgccgcac cgataaacac
     1321 ggcaaacacg cccgccgcca gcgagatttg cgcaccaacc aggacacggc tgaaaatgtc
     1381 gcgccccagt gaatcgacgc caaaccagtg ctgcaaagaa ggtccgttat tcagattgtc
     1441 ataatcaaaa taattttcgg cgtcataggg agcgatccag cgtgcaaaaa tggccaccac
     1501 aatcaataaa ataacgaata atgcggcggt catcgccata tgctgacggc gaaatcgtcg
     1561 ccagaattca tgccacggtg tacgcacctg gtcaggtttg accagtggca tggcgtttaa
     1621 caccgcctga cgtcgccagt taaatagtcg catccttact tgtacctgat agccgggtta
     1681 atggcggcgt aaagcacatc caccactaag ttgataagaa taaattccag cgagaaaagc
     1741 agaatttccg cctgaatcac cgggtaatcg cgcatttcta cggagtcaac gagtaagcgt
     1801 ccaagtccgg gccagttgaa aactttctcc accacgatag aaccaccgag caaaaagcca
     1861 aactgtaagc ccatcatggt cactaccggg atcatcgcgt tacgtagccc gtgtttgagg
     1921 acaacccagg tttcgctcac ccctttcgcc ctcgcggtac gcatataatc ttcgcttaaa
     1981 acatcgacaa acgacgcgcg ggtaaagcgc gccatcacgg ctgccactgc cgcgccgagc
     2041 gtcagggagg gtaaaatgta gtgctgccaa ctgtctgctc ccacggtagg cagccagccc
     2101 agttcaacgg agaatacctg aattaaaagc atccccagcg caaatgccgg aaacgagatc
     2161 cccgacaccg caatggtcat actcaatcga tccggccaac gattacgcca gacggcggcg
     2221 ataattcccg ccgccatacc aaatatcacc gcccagacca tactggttat ggtcagccac
     2281 agcgttggca taaagcggct ggcaatctca tcggcaaccg gacgacgcga caccatcgac
     2341 aggccaaaat ccccctgcac agcattgctg atatagtgcc agaactggtg atacagcggc
     2401 tgatccaacc ccagctgctg acgcaccagt tctataacct gcgcatcagc ttcgagcccg
     2461 gcaatcaatc gcgccggatc gccgggcagc atatgcacaa ataaaaacac cagcaccgag
     2521 acgataaaca gcgtcggaat caaccccagt aagcgtttga taacgtaatt aagcattcca
     2581 ctccctgcgt tgcttattgc aaatccgcgt cttcaaagct gaagccggtg tctggcatga
     2641 tccaaaaacc ggtcaggttt ttactgtgtg ccgacaccag tttttctacc accagcggga
     2701 tccacggcga ctcttgccag atgatatcct gcgccgcctt atacaagcgg gtcttttccg
     2761 ccggatcatt agttttcagt gcctgagcca ggaagtcgtc cacctgttta ttgctgtaaa
     2821 atgcggtatt aaacagcgtc ggtggccagt tttgcgaggc gaacagtggc gatagcgccc
     2881 agtccgcttc gccggttgaa gccgaccagc cagtgtagaa catccgcacg ccgctctctt
     2941 tttgcccttt accttcaact tctgccgccc gctgtccggc atccatcgcg gtcacctggg
     3001 ctttaatccc gacctgcgct aactgctgct gggtaaattg cagcactttc tgcgcggtgc
     3061 tgtggttatg tgacgaccac agcgtggtac tgaaaccgtt gggatatccc gcctctttca
     3121 gtaattcgcg cgctttcact ggatcgtaag gccacggttt ataactttgc gcgtaggcga
     3181 tacttggcgg taccacacca gtagctggcg ttgcatagcc cgcaaaggca actttcacca
     3241 gcgccggacg gttaatggcg taattcagcg cctcacggac cttcgggtta tcgaacggct
     3301 tttgcgtcac gttcatactg atataacgct gcataattga cggactggcc atcaactcga
     3361 tatttttgtt tttctccagc agtgtggcct gctcgtaggg aatggggaaa gcaaactgcg
     3421 cttcaccggt ttgcagcatt gccgcgcggg tgttgttatc cgccaccgga cgccaggtta
     3481 tgctgtccag tttgggcaat cctggctgcc agtaacccgc gaattttttc accttcacaa
     3541 aatcggtctg attccaggta tccagttcat acggtccggt tcccaccgga tgaaaaccaa
     3601 tctccttgcc atatttttcc agcgctgccg gtgaaatcat cgcggtcgcc ggatgggcaa
     3661 gaatattaat aaacgctgag aacggctgtt tgagggtaat ctttaccgtt gtcggatcga
     3721 tcgcctccgt tttggcaata ttcttataca ggttatagcg tttaagatga ttcgccggat
     3781 cgctggcccg gtccagattc gcttttaccg ccactgcgtt gaaatcggtg ccatcctgga
     3841 atttaatgcc ttcccgcaat ttcacggtgt aagtaatgcc gtcatcggaa acggtataac
     3901 tctccgccag cacgtttttc agtttcatct ctttatccag accgaacagc ccctggtaaa
     3961 acgatttcgc tacggcctga gataacgtat cattcgcgtc atacggatcg agcgtggtga
     4021 aattcgatcc taccgccacc accacatctt tggcagcgaa tgcacaagat gccatcaacg
     4081 ctgtcgcaat gcccagcgcc actaacccac tacggtgtac agcttttgcc attttattct
     4141 ccgcctgaat gttatctacg catgaatgcg tattctgatt gtggttgtgc gacgtaatgc
     4201 cccggcccga cgcattgcaa cgagacggct gccacctctt cgccacgcag atgaatattg
     4261 ctgggaagat cgtccgacag cagtacacgc tgcggtcgtt gtcgggacgg ttcagcgacc
     4321 ggaactgccg ccagtaattt acgcgtataa ggatgctgcg ggttttcgaa gaccgcgcgc
     4381 cgtggaccaa tttcaacaat ttgcccgaga tacatcaccg ccacacgatg actaatccgc
     4441 tctaccacgg ccatatcgtg ggagataaac agatacgcaa tgccgaaatc acgctggaga
     4501 tcgagcaaca agttgataat ctgcccgcga atagaaacat ccagcgccga aacggcttcg
     4561 tcggcaatga tcacttttgg attcaatgcc aacgcgcgag caatgcagat gcgctggcgc
     4621 tgaccgccgg aaaactcatg cgggtaacgc caggcatgtt caggtaacag gcccacgcgc
     4681 tccagcaacc acgcaacgcg tgcagccgcg tctttacctg gcaataaacc gtgtacacgc
     4741 agcggttcga taatcgaatc accgatggtc tgacgtgggt ccagcgaagc gtaagggtcc
     4801 tgaaaaataa actgaatatc ccggcgcaat gcctgaagtt tgccgggtga caaggtatcg
     4861 attctctgac cgttaaagat aatttcgccg ccctgcgatt cgaccaggcg cagcaacgcc
     4921 cgcccggtag tggatttacc gctgccagac tcgcccacca gcgatagcgt ttcgccaggc
     4981 cagagatcaa aactgacttt ctcaacggca tgcacttccc gcgttacgcg attcaacaaa
     5041 ccgctgcgca aagggaaacg ggtgacaaga ttacgcactc gtaaaacagg ttcgccatcc
     5101 accaccgttt tctgctcgat ggggggggcc tgtttcgctg gatgttcaag cgatatcaac
     5161 gggaaacgtc ggggataatc caaccctttc atcgcaccaa gttgcggaac agcagctaac
     5221 agcgcacggg tgtaaggatg ttgcggtgca tgaaaaatct gttcgacggt acccgtttcc
     5281 accgcctcgc cctgatacat caccagtacc cgatcggcaa tctctgccac cacgcccata
     5341 tcgtgagtga taaagataac gcccatcgac atctcttttt gcaatacttt gattaattgc
     5401 aggatctgcg cctgaatagt gacatccagc gcggtggttg gctcatcggc aatcagcacc
     5461 gccgggcggc atgacagcgc catcgcaatc atcactcgct ggcgcatccc gccagagagt
     5521 tgatgcggat aacgtgaaag aatggtttgt gcctcaggaa tgcgtacctg atccagcatc
     5581 cgcttcgcct cgaccatcgc ttcttcacga ctggcgttct gatgcagacg aattgattcg
     5641 gcaatctgtt cacccacagt aaataccggg ttcagcgatg tcatcggctc ctgaaatatc
     5701 atcgccatat ccgcaccgcg aacatggcgc atttgtgcag cgctctgctc gctaagttca
     5761 atcacttcgc gactgcgccg ctgcaacagc attttatcgc actgtactaa accgcccgcc
     5821 tgttccaaca ggcgcatcaa tgccaacgca gtcactgact taccggagcc ggattcgcca
     5881 acaattgcca gcgtctcacc gcgttgcaga ctaaaagaga gattgcggac cgcagctatt
     5941 ttctgctggt cctgcataaa ggcaatattc agattttcaa ccgccagcac attaccggca
     6001 tcaagttcat cactgtgtgg caacggtgtc ccctttttca cggtagatac cggtggttgg
     6061 cgtatcgcct gcgtagcccc aggcgcgata cattccttcg gtgttaaacg gtagcgcgac
     6121 attcccttca tggtcgatag cgattaagcc accgctaccg ccaagcgcag ggagtttttc
     6181 cattactacc cgctcgcagg cttccgcgag acttaatccg ccgtaatcca ttaacgcggc
     6241 gatgtcatat gccgccagcg cgcggatgaa gacttcgccc gtgccgatac aagaaaccgc
     6301 cacactggcg ttattggcgt agcatccggc acccactaag ggactatcgc caactcgtcc
     6361 gggtaattta ttggtcattc cgcctgtgga cgtggctgcc gccaaattgc cgtctaaatc
     6421 caacgccacg gcccccacgg tgcccatttt ctgtttttca tccagtggcg caccgctatg
     6481 gtcgaggacg gttgcccctt ccttgcgcgc tgccagtagt tgttcataac gcaaaggcgt
     6541 ggagaaaatc tccggcgaga cgcgctccat gccacgagca aacgcaaaat tttctgcccc
     6601 ttcgccaatc atcatcacat gcgggctttg ctccatcacc agccgggcgg caagaaccgg
     6661 attacgcaga tgactaacgc ccgccaccgc accggctttc agggtgttac catccatcac
     6721 acaggcgtcc agttcatggg tttcatcacg cgtaaagaca gcgccaattc cggcgttaaa
     6781 cagtggacac tcttccagca gacgcaccgc ttccgtcacc acatccagcg cactttcgcc
     6841 cgcttccagc attttctgcc cggtttcaac aatggcagac aacgcctcaa tgtagcgtaa
     6901 ttcctgttgc agactcatct gcgcgcggct aattgcacct gcgccaccat gaattgcaat
     6961 gactgctttg cccatagtat tcgtccatta agggtggctc gccacttttg ctataaatat
     7021 cagaattttt atgaattact cattcaattt ttgaatatag aaagatggat atggcatgta
     7081 aaggcaggag ccaacgctac gcccgaggtg gcatacagtc tgtggcgttt ttctgacata
     7141 ataggcaaat tcgattttgc ctccgcagga gtgttttcat ggaatttacc accggattga
     7201 tgtcgctcga caccgcgctt aatgagatgc tttctcgcgt caccccactg accgcccagg
     7261 aaacgctgcc actggtacag tgttttggtc gtattctggc gagcgatgtc gtttcgccgc
     7321 ttgatgttcc ggggtttgat aactccgcaa tggacggcta cgcggtgcgt ttagccgata
     7381 ttgcctccgg gcaaccgctg cccgttgccg gtaaatcctt tgccggtcag ccgtatcatg
     7441 gtgaatggcc tgcgggcacc tgcattcgta ttatgaccgg tgcgccggtg ccggaaggct
     7501 gcgaagcggt ggtgatgcag gagcagactg aacaaacgga caatggcgtg cgttttactg
     7561 ctgaagtgcg tagcgggcaa aatattcgcc gtcgcggtga agatatctct gcaggtgcgg
     7621 ttgttttccc ggcgggaact cgcctgacta ccgcagagct gccagtaatt gcttcactgg
     7681 ggattgccga agttccggtg attcgtaaag tgcgtgtagc gcttttttct accggtgatg
     7741 aactccagtt gcccggtcag ccgctgggcg acggccaaat ctacgacacc aaccgtctcg
     7801 ccgtacacct gatgttagaa cagctgggtt gcgaggtaat caacttaggg attatccgcg
     7861 acgatcccca tgccctgcgc gccgcattta ttgaagccga cagccaggcg gatgtagtga
     7921 tcagttccgg cggtgtttca gtgggtgaag cggattacac caaaacgatt cttgaagagc
     7981 tgggggagat cgccttctgg aagctggcga ttaaaccagg taaaccgttc gcgttcggta
     8041 aactcagcaa tagctggttc tgcggcctgc cgggcaaccc ggtttcagcg acgctgacct
     8101 tctatcaact ggtacagcct ttgctggcaa aactaagcgg caacaccgcc agcggcctgc
     8161 ccgcgcgcca gcgcgtacgc acagcgtccc gcctgaaaaa aacgccagga cgtcttgatt
     8221 tccagcgcgg cgtgctgcaa cgcaacgccg acggtgaact tgaagtgacg accaccggac
     8281 atcagggttc acatatattt agctccttta gtctcggcaa ctgctttatc gtgctggaac
     8341 gcgatcgcgg caatgtggac gtgggcgaat gggtggaagt agaaccgttt aacgcgttgt
     8401 tcggaggcct gtaatggcgg aactcagcga tcaggagatg ctgcgctaca accggcaaat
     8461 cattctgcgc ggttttgatt ttgacggtca ggaggcgcta aaagattctc gtgtgctgat
     8521 cgtcggcctc ggtggactcg gctgcgcagc ttcgctgtat ctggcaagcg ccggtgtcgg
     8581 caacctgacg ctgctcgact tcgacacggt ttcgctctcg aatctgcaac gccagacact
     8641 gcacagtgat gccacggtcg ggcaaccgaa ggtggaatcc gcccgtgacg ccctgacgcg
     8701 gatcaaccca catatcgcga ttacgccagt caatgcactg ctagatgacg cagaacttgc
     8761 agcattgatt gctgaacacg atctggtgct cgactgtacg gataacgttg cggtacgtaa
     8821 tcaactgaac gcaggctgtt ttgccgcgaa ggtgccgctg gtttccggcg cggcaattcg
     8881 tatggaaggg caaatcacgg tcttcactta tcaggacggc gaaccgtgct atcgctgcct
     8941 tagccgtttg tttggcgaga acgcattaac ctgcgtagaa gcaggcgtga tggcaccgtt
     9001 gatcggcgta attggttcgt tgcaggcaat ggaagcgatc aaactgctgg caggttatgg
     9061 caaaccgacc agcgggaaaa tcgtcatgta cgatgcgatg acctgtcagt ttcgcgaaat
     9121 gaaactgatg cgcaatccgg ggtgtgaggt gtgcgggcag taattgccac ctccaggcct
     9181 gataagacgc gacaagcgtc gcatcaggca tttgctccag acgccagatg cggcgtgaac
     9241 gccttatctg gcctacggtt cgggcacaga tttccaagcg cggcaggcat cggtgcactc
     9301 cgccacgtca tcagcaattt atgaggatgc agaacgtccg gttaaatcga cgttctgcca
     9361 aacgctccct gccagtcctg ctcaaacttc gccacagcgg cttccaccgc cggatagcta
     9421 atcatctgtt gtgccacatc cagtggcaga gtaattgatt cacatcctgc cagtaagcag
     9481 tccagcgcct gacgcggggt tttgaaactc gctgccagca ctttcgcctg cggcgcatgc
     9541 attttcaata actggtgtaa gtcggtcaca gtctgaatgc cgctaccgcc ctgagcatca
     9601 atacgattaa cgtaaggcgc aacatattcc gcacctgcca gcgccgacag caacccttgt
     9661 gctgcgccat ataccgcggt tcccagcgtt ggaatccctt ccgcttttaa catcttaata
     9721 gctgccagcc cctcggcggt caccggaact ttcaccacga tatccgcaat aatagaacgc
     9781 aatttacgtg cgtcattcac catcccttca gcagtggtag ccattacctg ggcaaacaga
     9841 cgcccctgac cgcccatcgc ttcatgaagt tgcggaagca caacatccag cggtttttta
     9901 cccgcggcga taatgcttgg gttagtggtc acacccgcca gcggaaaaat acgtgacagc
     9961 gccttcaccg caacaacgtc tgaagtatcc agatacagtt ccataaccat cctcaaaatg
    10021 tactttttgt ctgaatgtag actatcacgg gaaaactctc tgaacagttg acgcacatca
    10081 atctaacttt cattcgaaag tattttaatg tttgaacgaa acagagaggc gactatgatt
    10141 ttcaatattc agcgctactc gacccatgat ggccccggta tccgcacggt cgtatttctt
    10201 aaaggctgtt cgctgggctg ccgctggtgt cagaacccgg aaagccgcgc ccgcacgcag
    10261 gatctgctgt atgacgcacg actgtgtctg gaaggctgcg agctgtgcgc taaggccgcg
    10321 ccggaagtga ttgagcgcgc gctgaatggt ttgcttattc atcgggaaaa gttaaccccg
    10381 gagcatctga cggcgttaac cgactgctgt ccgacacagg cattaaccgt gtgtggtgaa
    10441 gtgaaaagcg ttgaggagat catgacgacc gttctgcgcg ataaaccgtt ttacgatcgc
    10501 agcggcggcg gtttaacgct ttcgggtggt gagcccttta tgcagccgga aatggcgatg
    10561 gcgctactgc aagccagcca cgaggcaggc attcatactg cggtagaaac ctgtctgcat
    10621 gtgccgtgga aatatatcgc cccttctctg ccctatatcg atctgtttct tgccgattta
    10681 aaacacgttg ccgacgcgcc gtttaaacag tggaccgacg gtaacgccgc cagagtgctg
    10741 gataacctga aaaaactcgc cgcagcgggc aaaaaaatca ttatccgcgt gccgctgatt
    10801 cagggcttta atgccgacga aacctctgta aaagctatta ccgattttgc cgccgacgag
    10861 ctgcacgttg gcgaaattca tttcctgccc taccacacgc taggcatcaa caaatatcac
    10921 ttacttaatc tgccctatga cgccccggaa aaaccgcttg atgcgccaga actgctcgac
    10981 tttgcccagc agtatgcctg ccagaaaggg ttaaccgcga ccttacgagg ataacaacca
    11041 tgaccacact gaaactggac acgctcagcg accgcattaa agcgcacaaa aatgcgctgg
    11101 tgcatattgt gaaaccgcca gtctgtaccg agcgcgcgca gcactatacc gagatgtatc
    11161 aacaacatct cgataagccg atcccggtac gtcgcgcgct ggcactggcg catcacctgg
    11221 cgaatcgcac catctggatc aaacacgatg agttgatcat tggcaaccag gcaagcgaag
    11281 ttcgcgccgc gccgatcttc ccggaatata ctgtctcgtg gatcgaaaaa gagattgatg
    11341 atctggcaga tcgtcccggt gctggctttg cggtgagcga agagaacaaa cgcgttctgc
    11401 atgaagtgtg cccgtggtgg cgcggtcaga ccgtacagga tcgctgctac ggcatgttta
    11461 ccgatgagca aaaaggtctg ctggcgaccg gaatcattaa agcggaaggc aatatgacct
    11521 ccggcgatgc gcacctggcg gtgaatttcc cgctgctgct ggaaaaaggt cttgatggtc
    11581 tgcgcgagaa agtagcggaa cgtcgctcgc gcatcaatct gacggtactg gaagatttgc
    11641 acggcgaaca gttcctgaaa gcgattgata tcgtgctggt ggcagtcagc gaacacattg
    11701 aacgttttgc agccctggcg cgtgaaatgg ccgcgaccga aacccgcgaa agccgtcgcg
    11761 atgaactgct ggcgatagca gaaaactgcg atcttatcgc ccaccagccg ccgcagactt
    11821 tctggcaggc gctgcaactg tgttacttca tccaattgat attgcagatc gaatctaacg
    11881 gtcactcggt atcgtttggt cgtatggacc agtatctcta cccgtactat cgccgcgacg
    11941 ttgaactcaa ccagacactg gatcgcgaac acgccatcga attgctgcat agctgctggc
    12001 tgaaactgct ggaagtgaac aagatccgct ccggctcaca ctcaaaagcc tctgcgggaa
    12061 gtcctctgta tcagaacgtc accattggcg gacaaactct ggttgatggt caaccaatgg
    12121 acgcggtgaa tccactctct tacgctatcc tcgaatcctg cggtcgcctg cgttccactc
    12181 agcctaacct cagcgtgcgt taccatgcag gaatgagtaa tgatttcctc gacgcctgcg
    12241 tacaggtgat ccgttgcggc ttcgggatgc cggcgttcaa caacgacgaa atcgtgatcc
    12301 cggaatttat taaactcggt attgaaccgc aggacgctta cgactacgca gcgattggtt
    12361 gtatcgaaac cgccgtcggt ggcaaatggg gctatcgctg taccggcatg agctttatca
    12421 acttcgcccg cgtgatgctg gcggcgctgg aaggcgggcg tgatgccacc agcggcaaag
    12481 tgttcctgcc acaagaaaaa gcgttgtcgg caggcaactt taacaatttc gatgaagtaa
    12541 tggacgcgtg ggatacgcaa atccgttatt acacgcgtaa atcaatcgaa atcgaatatg
    12601 tcgtcgacac catgctggaa gagaacgtgc acgatattct ctgctcggcg ctggtggatg
    12661 actgtattga gcgagcgaaa agtatcaagc aaggcggcgc gaaatatgac tgggtttctg
    12721 gcctgcaggt tggcattgcc aacctcggca acagcctggc ggcagtgaag aaactggtat
    12781 ttgaacaggg cgcgattggt cagcaacagc tggctgccgc actggcggat gacttcgatg
    12841 gcctgactca tgagcaactg cgtcagcggc tgattaacgg tgcgccgaag tacggcaacg
    12901 acgatgatac cgtcgatacg ctgctggctc gcgcttatca gacctatatc gacgaactga
    12961 aacagtacca taacccgcgc tacggtcgtg gtccggttgg cggcaactat tacgcgggta
    13021 cctcgtctat ctccgctaac gtaccgtttg gcgcgcaaac tatggcaaca ccggacgggc
    13081 gtaaagccca caccccgctg gcagaaggcg caagcccggc ctccggtact gaccatcttg
    13141 gccctactgc ggtcattggc tcagtgggta aactgcctac ggcagcgatt ctcggcggcg
    13201 tgttgctcaa ccagaaactg aatccggcaa cgctggagaa cgaatctgac aagcagaaac
    13261 tgatgatcct gctgcgtacc ttctttgaag tgcataaagg ctggcatatt cagtacaaca
    13321 tcgtttcccg cgaaacgctg ctggaggcga aaaaacatcc ggatcagtac cgcgatctgg
    13381 tagtgcgtgt cgcgggctat tccgccttct tcaccgcgct ctctccagac gcacaggacg
    13441 atatcatcgc ccgtactgaa catatgctgt aatctcctcg gcccggcggc gtcgtcgccg
    13501 ggctaaatca ttcacatcat caatttcatc cttactttca ttcgaaatat aatttgtgct
    13561 ctgcgtcaca ttgttattac actgttacgg tttcattttc gagccaggag cacacctatg
    13621 agcgtaaaag ttatcgtcac agacatggac ggtacttttc ttaacgacgc caaaacgtac
    13681 aaccaaccac gttttatggc gcaatatcag gaactgaaaa agcgcggcat taagttcgtt
    13741 gtcgccagcg gtaatcagta ttaccagctt atttcgttct ttcctgagct aaaggatgag
    13801 atctcttttg tcgcggaaaa cggcgcactg gtttacgaac atggcaagca actgttccac
    13861 ggcgaactga cccgacatga atcgcggatt gttattggcg agttgctaaa agataagcaa
    13921 ctcaattttg tcgcctgcgg tctgcaaagt gcatatgtca gcgaaaatgc ccccgaagca
    13981 tttgtcgcac tgatggcaaa acactaccat cgcctgaaag ctgtaaaaga ttatcaggag
    14041 attgacgacg tactgttcaa gttttcgctc aacctgccag atgaacaaat cccgttagtg
    14101 atcgacaaac tgcacatagc gctcgatggc attatgaaac ccgtcaccag tggttttggc
    14161 tttatcgacc tgattattcc cggtctacat aaagcaaacg gtatttcgcg gttactgaaa
    14221 cgctgggatc tgtcaccgca aaatgtggta gcgattggcg acagcggtaa cgatgcggag
    14281 atgctgaaaa tggcgcgtta ttcctttgcg atgggcaatg ctgcggaaaa cattaaacaa
    14341 atcgcccgtt acgctaccga tgataataat catgaaggcg cgctgaatgt gattcaggcg
    14401 gtgctggata acacatcccc ttttaacagc tgacctctct accggagccg tgcgctccgg
    14461 ttttcccttc tttttacatc ctgtacatca catcaaatca ttaaacttgc attaacttat
    14521 ttataactta tattgtcgca tgcaaatcag aatggtttcg aatgaaagtt aatgagaggt
    14581 ggttatggct tctactttta ccagcgacac attgcctgcc gatcacaaag cagctatccg
    14641 tcagatgaag cacgcgctgc gggcgcagct tggcgacgtc cagcagatct ttaatcagct
    14701 aagcgatgac attgccacgc gagtggctga aatcaacgca ctcaaagcac agggcgatgc
    14761 cgtctggccg gtgctgtctt atgccgatat caaagcaggt catgttactg cagagcagcg
    14821 cgaacagatt aaacgtcgcg gttgtgcggt gataaaaggc catttccccc gcgaacaagc
    14881 gctaggctgg gatcagtcga tgctggacta tctggaccgc aaccgctttg acgaggtcta
    14941 caaaggcccc ggcgataatt tcttcgggac gctcagcgct tcacgtcccg agatttaccc
    15001 catctactgg tcgcaggcgc aaatgcaggc ccgccagagt gaagaaatgg cgaatgcgca
    15061 gtcgtttctc aatcgtctgt ggacatttga aagtgatgga aagcaatggt ttaacccgga
    15121 tgtgagcgtc atctaccctg accgtatccg ccgccgtccg cccggaacga cctccaaagg
    15181 tcttggagcg cataccgact ccggggcgct ggaacgctgg ctgcttccag cgtatcagcg
    15241 cgttttcgcc aacgtcttta atggcaatct ggcgcaatat gatccctggc atgcggcaca
    15301 tcgtacggaa gttgaagagt acacggtgga caacaccacc aaatgttccg tgtttcggac
    15361 attccaaggc tggacagcgc tctctaatat gctgcctggt caggggctgc tgcacgttgt
    15421 gcccattcct gaagccatgg cgtacgtact gttacgtccg ctgcttgatg atgtgccgga
    15481 ggatgaactg tgcggcgtag cgcccggaag agtgttgccg gtatcagagc aatggcatcc
    15541 actgttaatt gaggcgttaa ccagcattcc aaaactcgag gccggagact ccgtctggtg
    15601 gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat caacagggtt ggggcaacgt
    15661 gatgtacatt cctgcggcac caatgtgcga gaaaaatctt gcctacgcgc acaaggtgaa
    15721 ggccgcactg gaaaaaggcg catcgccggg cgacttcccg cgcgaggact atgaaacaaa
    15781 ctgggaagga cgctttacgc ttgccgacct caacattcac ggtaagcgag cgttgggaat
    15841 cgacagctga gtgtcgtaca gcccttcccg ttccacggcg gtgttccctt ttcctgggcg
    15901 aacccgccgc actgactccc gcaccgccaa tcgtccgtgc agcagcagcg agcccaccgg
    15961 agcatccggg cgaatcagcc gctgttgcaa catttgcacc gcttcattgc ctagctcatc
    16021 gcgtggcaca tgcacagccg tcaggggaac atcctcgatt gccgcaaggt taaagccgtc
    16081 gatactcatc accgagacat cctgtggtac gcgcagacca cgacgatgta gcgcatttac
    16141 cgttcccgcc gccataaaat ccccgccgac cagaaacgcc gtcggcaacg tcttgccatt
    16201 ggcggcatcc agccacgctc cgaccttttc ttccgcttct ttagcgctaa aactgctgat
    16261 attcagcaaa tgacggtcat ggtgaaaggc cagattatgc tgacgccatg attctttaat
    16321 ccctgcaaac cgctgctcca tagtgtagcg gcgcaggcac atcacattca tcacctcccg
    16381 gtgacccatt tcaaacagat agcgggcggc gaacgcgcca attaaccgat gatccggcgc
    16441 aatcgccggg aggcgcattc gttcatcacg gcagttgatc agcacacaag gcttagcgag
    16501 atcggcggcc agatcatgga tgtgcggatc gtcaatcccc agcaggatcg ctgcctgcgt
    16561 atctgcttca ttcatccgcg agagaaagag cgcgggatcg ctgtcaaact cctccagcgc
    16621 gcagtaacgc agccgcactt catgcggcga taacgctttg ctgatgctct ggatcacacg
    16681 gtaataaaag atatcagatc gttggtcaaa agcgcgttgc ggcgcgaaaa taatcaggtt
    16741 gttcagcaac atacgacctg ctgccatgcc atccatcacg cccaactcac gcgcgcaggc
    16801 cagcacctgt tcgcgagcgc gtgcactggt attcgctttg ccagccagca cccgcgaaac
    16861 cgtactcggc gatagcccgg tacgtgcggc gatatcggcg attttcagct tcttgtccat
    16921 tttgtgatct gcctctaatt cggaaatgac agaattttca tgagcaaaaa agcccattct
    16981 gtctggcatt aaatccattt cagtaatgta tatcagtcac tcatggaaaa acgtgcacaa
    17041 aacccgcttt ctttcgcttt tagggtctct tactgtgaat tggtagaaca acttccatac
    17101 aaaaataatg gaaaaacgaa caataaagac tttatttatc aaaaagttaa aagtggtgta
    17161 acaactccat tctgacgttg ccgcacaaac cctataccat tctgtggagt gcatatgagt
    17221 cagggtatca ataacgatat gactgccagt aaaccgcgcc atatcgtcaa aaatctgcgt
    17281 tggtggatac tggtcctttt tttgctcggt gttactgtca actacatcac gcgtaactcg
    17341 ctgggtattc tcgcccccga actgaaaagc agcctaggga ttaccaccga acaatattcc
    17401 tggattgtag gtgctttcca gctcgcctat accctattcc aaccgttgtg cggatggctg
    17461 attgacgtca ttggcctcaa actgggcttt atgatctgcg cgggactgtg ggcactggcc
    17521 tgtctgatgc atgcgggggc gggaaactgg ggacatctgg cgctgttgcg cttctttatg
    17581 ggcggtgcgg aagccgcggc aaccccagca aacgccaaaa ccatcggtga atggtttcca
    17641 aaatcagaac gcccgatcgc cgcaggctgg gcaggcgtcg gtttttccat cggtgccatg
    17701 ctggccccac cgattatcta ttttgcccac gcttcattcg gctggcaggg tgcatttatg
    17761 ttcactggca tcctggcgat gctgtgggtg gtgctgtggt gggcgttcta tcacagcccg
    17821 gataagcatc ctaacctcag caaagccgag ctggacttta ttcgccagga taacgaagcg
    17881 cccccggtca agttaccctt tctgaccgca ctgaaaaccg tgggcaaaaa taaacgcttt
    17941 tacggtatcg ctatcccggc ctttatggca gaaccggcgt gggcggtgat gagtttctgg
    18001 gtgcctctat acctgtccaa aacctatggc atggacctga agcagatcgc attattcgcc
    18061 tggttgccgt tcctcgccgc tgatcttggc agtattgcca gcggctatct gaccaaactt
    18121 tacacccgcc tgtttgggct gagtcgtgtg aactccgttg tcgcatcatc ggtcagtggc
    18181 gcattcttga tggtttcgct ggcggtgatg gcctttacca ataacccgta tatcgccatc
    18241 gtccttatct ccatcggcgg cttcggtcac cagatcatct cctgcatgct gagcgcactg
    18301 gtggtggaat ccttcgacaa aggtcagatg gccactgtta acggcatgcg cggttctgcc
    18361 gcgtggatcg caagtttcct gttctccctg attatcgggg ttaccgctga caaaatcggc
    18421 tttaaccccc tgtttatcgc catgggcttc tttgacctga ttggcgcttt cttcctggtc
    18481 acctttattg ctgaacgtcg cgcaaaacgc gcctgagagt ggatgtaaac atgaaaacgc
    18541 tgaaaaactg gaccctgcac aaacagtctg cccaccacgt tgaactgctg gtcgatggcc
    18601 agcatatcct gtgcctgtac gtgctggaag aaaatctgtt ccgcgtgctg ttaaaacgca
    18661 aaggcgagct ggcgctagat cgcacctgga gcattgctcc ggcgcaggat gtgccatggg
    18721 aagggcgcag ccgcgaagat ctcagcggct ttagcctgcc cgcgtggaca gtgatccagc
    18781 acgacgacac cgtggttatc gccaccgagt cactgcgtgc aaccgttcac cagccgctgt
    18841 ggctggagtg gcactatcgt aacaacgaag gcgaatggct gccgttggtg aatgaccgcc
    18901 cgaccagcgc ttatctgctg aatgcccacg gcgatggcgt ggcacactat cagagccgtc
    18961 gtaaagatga acgcttttat ggcctcggag ataaagccgg cgatctgcaa cgcaacggta
    19021 aacgttacga gatgcgtaac ctcgacgcga tgggctacaa cgccgttagt actgacccgc
    19081 tgtacaaaca cattccattc accatcaccc gccgcgatga cattagcttc ggcctgtttt
    19141 atgacaacct cagcagctgc tggctggatc tgggcaacga gatcgacaac taccacaccg
    19201 cctatcgccg ctggcaggcg gaagcgggtg atatcgacta ctacctgttt accggcgaac
    19261 gcgtacttga tatcaccaaa gccttcgtac gcctgaccgg aaaaaccctg ttcggaccga
    19321 aatggagcct gggctacagc ggttcaacta tgcactacac cgatgcgccg gacgcacaaa
    19381 atcagttgat gaactttatc cgcctgtgtg aagaacatgc aattccctgc gattcgttcc
    19441 agctctcttc cggttatacc tcaattaacg gcaaacgcta tgtgttcaac tggaactacg
    19501 acaaagtgcc gcagccgaaa gtgatgagcc aggcatttca caatgctggt ttgcacctgg
    19561 ccgccaatat caagccgtgc ctgttgcagg atcacccacg ctataacgaa gtggcagaaa
    19621 gaggactgtt catccgtgat tcagaaacag atgaaccaga acgttccagc ttctgggatg
    19681 acgaaggttc taacctcgag tttactaatc cgcagaccat ccagtggtgg caggaaggcg
    19741 tgaccacaca gcttctggag atgggtatcg actccacctg gaacgacaac aacgaattcg
    19801 aagtatggga cggtgaagca cgctgtcacg gctttggtca gacgatcgcg atcaaacata
    19861 ttcgcccagt gatgccactg ctgatgatgc gagcctcgat ggaagcccag cagcgtttcg
    19921 ctccggaaaa acgtccgtac ttgatctccc gctccggctg cgcgggaatg cagcgctacg
    19981 tgcagacctg gagtggcgat aaccgcacta gctgggacac tcttcgctac aacacccgca
    20041 tgggtctggg gatgagcctc tccgggctgt ataacctcgg tcacgacgtc ggcggtttct
    20101 ccggtgataa gccggacccg gaactgtttg tacgctgggt acaaaacggc gtgatgcatc
    20161 cgcgctttac gattcactca tggaacgatg accataccgt aaacgaaccg tggatgtatc
    20221 cgggcgtcac tccggcgatc cgcagcgcta ttgagttgcg ctatcgcctg ctgccctact
    20281 tttataccct gatgtggcag gcatacgccg atgatgaacc aatgctacgt ccgactttcc
    20341 ttgaccacga gcacgacgtt cagacttttg aagagtgtga tgacttcctg cttggtcgcg
    20401 atattttggt tgccagcgtc gtagagcaag gcgagcgcca gcgccgtgta tggctgccag
    20461 ataacgaaac tggctggtac gatttctaca acggcgaatg gttcagtggc ggtcagtgga
    20521 tcaccctcga cgcaccgctg gagaaactgc cgctgatggt tcgtgccggg gctggtctac
    20581 cgctcagcaa acgcatcacc tatgtgagcg cagaacaaga cgatacccgc gaactgaaac
    20641 tgttcccgct gaaaggtgtg ggcacaacgt ccggcctgct gtttgaagac gatggcgaaa
    20701 gctggggcta ccaaactggt aatgcgcttt gggtggaatg ggaaatggtt tgcgatgggg
    20761 cgaccatcaa cctgaaagtc aacgcgcgcg gggattatcg tcctgcatgg agtgcactga
    20821 aagtgtcatt accattggaa gaaaaacgca cgctgctggt caatggtgtt gaagggagtg
    20881 aatggatgcg ttaaatcatt accggggccg ggaggcgact gcaccttacc cggtccagcc
    20941 atcgaatcct ttacctcagt atttcacgct gcaggaaggc agcaaacctg taaaccccgg
    21001 gagcttaaat aagtaagtga ccggggtgaa caggtgaagc caatgcatct gccgcatgaa
    21061 agacggcggg gaattaagcg tcgatccctt tactacgcag gtaatcttcg taattaccgc
    21121 taaagtcgat cgcgcgttcc ggggtgattt ccagaatgcg ggtcgccagg gagcttacga
    21181 actcacggtc gtgtgaaaca aagatcagcg tgccctgata cagttccagc gccatgttca
    21241 gtgactcgat ggattccata tccaggtggt tggtcggttc gtccatgatc agaatgttcg
    21301 gcttctgcat cattaactta ccaaacagca tccgcccttt ttccccaccg gaaagcactt
    21361 tagctggctt tttgatgtcg tcctggctga acagcaaacg accgagaatg ctgcgtaccg
    21421 cctgctcgtc atcgccttcc tgcttccact gactcatcca ttcgaacacg gtcagatcat
    21481 tttcaaactc atattcgtgg tcctgggcgt agtaaccaat gcgcgcgttt tcagaccatt
    21541 ttacggtgcc gctgtccggt tgcagatcgc ccaccagcgt tttcagcagc gttgatttac
    21601 cgacgccgtt ggtacccagt accgcaagtt tttcaccgac ctccagcagc aggttgagat
    21661 ttttaaacag tggaccgtta tcaaaccctt tggtcagacc ttccacttcc agcgcgttac
    21721 ggaacagttt cttatcctgt tcaaaacgga tgaacgggtt ctgacggctg gaggctttca
    21781 cctcttccag tttgatttta tcaatctggc gcgcgcgcga agttgcctgg cgagatttcg
    21841 aggcgttggc gctaaagcgg ctaacgaaag attgcaactc agcaatctgc gctttcttct
    21901 tggcgttatc ggccagcaga cgttcacgcg cctgggtcgc cgccgtcatg tactcatcgt
    21961 agttacccgg ataaacgcgc agctcgccgt aatccagatc cgccatgtgg gtacagacca
    22021 tgttaaggaa gtgacggtcg tgcgagatga tgatcatggt gctgtcacgc tcgttcagca
    22081 cctgttccag ccagcgaatg gtgtcgatgt cgaggttgtt ggtcggttcg tcgagcagga
    22141 gaatctccgg atcagcaaac agcgcctgcg ccagaagcac acgcagtttc cagccaggag
    22201 caacttcact catcgggccg tagtgctgtt ccactggaat tcccacgcca agcaacagtt
    22261 caccggcgcg agcttccgca gagtaaccgt ccatttcgcc gtatttaact tccagatcgg
    22321 ccactttata gccgtcttcc tcactcattt ccggcaaagc atagatgcgg tcacgctcct
    22381 gcttcacttc ccacaactct ttatgcccca tgatcaccgt atccagcaca gtgaactctt
    22441 caaaggcaaa ctgatcctga cgcagcttac caatgcgctc gttgggatcg agggaaacgt
    22501 tacccaaagt cggctctaag tcgccgccga ggatcttcat aaaggtggat ttaccactac
    22561 cgttcgcgcc aatcaggccg taacggttgc cgccgccgaa tttgacggaa atgttttcaa
    22621 acaacggctt actgccgaac tgcatggtga cgttactgga aactaacaca ggcgtatcct
    22681 gaaaagagat atgacaaacc gcgcattatg ccataagtct gaaagaagat cctgctcctc
    22741 gcttaaggtc tacactcaag ccaaaggcaa aaaatgtgat ttcgtacaca tctgatttca
    22801 ctgtgagctg gaatgaactt ataatgcgct tccaatactc taatattctc aacccaatgg
    22861 cctgccaggc acaaaatctc gcttaacatg aatatgaaat tgaaaacatt attcgcagcg
    22921 gccttcgctg ttgtcggctt ttgcagtacc gcctctgcgg taacttatcc tctgccaacc
    22981 gacgggagtc gcctggttgg tcagaatcag gtgatcacca ttcctgaagg taacactcag
    23041 ccgctggagt attttgccgc ggagtaccag atggggcttt ccaatatgat ggaagcgaac
    23101 ccgggtgtgg ataccttcct gccgaaaggc ggtactgtac tgaacattcc gcagcagctg
    23161 atcctgccgg ataccgttca tgaaggcatc gtcattaaca gtgcagagat gcgtctgtat
    23221 tactatccga aagggaccaa caccgttatc gtgctgccga tcggcattgg tcagttaggc
    23281 aaagatacgc ctatcaactg gaccaccaaa gttgagcgta agaaagcagg cccgacctgg
    23341 acgccgaccg ccaaaatgca cgcagagtac cgcgctgcgg gcgaaccgct tccggctgtc
    23401 gttccggcag gtccggataa cccgatgggg ctgtatgcac tctacatcgg tcgcctgtac
    23461 gctatccatg gcaccaacgc caacttcggt atcggcctgc gtgtaagtca tggttgtgtg
    23521 cgtctgcgta acgaagacat caaattcctg ttcgagaaag taccggtcgg tacccgcgta
    23581 cagtttattg atgagccggt aaaagcgacc accgagccag acggcagccg ttatattgaa
    23641 gtccataacc cgctgtctac caccgaagcc cagtttgaag gtcaggaaat tgtgccaatt
    23701 accctgacga agagcgtgca aacagtgacc ggtcagccag atgttgacca ggttgttctt
    23761 gatgaagcga ttaaaaaccg ctccgggatg ccggttcgtc tgaattaatc ttcaaaactt
    23821 aaagcaaaag gcggactcat aatccgcctt ttttatttgc cagaccctag ttggccggga
    23881 gtataactaa caaaacatat cccaccaacg ccgcccacaa cagcatcggt attgaataga
    23941 gatggaaacg ccaccagatg cggcgatcgt tcgccatacg tagcgcaatc aaatttgcca
    24001 gcgatccggg taataaccca aagccaccta cgtttaccgc ccataccagt aataaagacg
    24061 gcggcacata gttcagcaac aatatggtac tcggcacgtt actgatcacc tgcgataaac
    24121 cgattgccgt taaccataac ccgggttcag atagatgact cacgttaccc aacacgcctt
    24181 gcaacgctgg aagctgggtc agtaaatgga cgtcgataaa catcgccata aacaccagca
    24241 gcagcgtcca gtccacactg agcaccacgc gacgtgccag cagcgcaaag cctgccgcca
    24301 caatcaccag tccccacagc tcttgtttga actccagcgc cgtcagaaag acgatataca
    24361 gccccagaca actccacacc agccgcggtt tccactccgg tgtttgcacc cccgtatggt
    24421 attgcagcgc ctttccaggg aaacaacacc agcacaggag caggagcgtc agcatcattg
    24481 cgccagccag cggtgccatt tgggcaataa atccggcaaa cgaaagacca gaacgtcccc
    24541 agataagaat attttgcggg ttgccaattg gcgtcagtag cgaaccagcg ttgactgcca
    24601 gcgcctcaaa aataatcagc cgattaaccg ggatctcaca cagtcttttt agcgtgatag
    24661 tcagcggaac aacaataaac agcgcgacat cgttggtcag aaaggtagaa agcagcgccg
    24721 ccgccagcac cataaacatc gccagccgac gctccgtagc aaagcggcgc accattttgc
    24781 gccccagcac atcaaaataa ccgcttaact ccacaccttt ggtcagcagc atcaggccgc
    24841 ttaaggtgat gatggtgtgc cagtcgatag cagcaggcca ggatttcggt gcaaagggca
    24901 cgaaaaagct taatccgata ccaacaagaa ttaataactg aaaaaaacga tcgccttgca
    24961 gcgtgcgtaa aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca aacgaaaagc
    25021 gtcgagcgtc tcttcactaa catggtgctc catgccttcc gcgtcgcgac gggcgatttc
    25081 cggactgacg cccaacacca acaagaaatt ttcgactatc tgatgtcgct cgcggctttc
    25141 ctgcgccagc ttctctcctt ctgccgttaa aaacacgcca cgccagggga tcatttcaat
    25201 cagccccatg gttgccagcc gcttaagcat tttagccacc gtcggttgcg aaactcccag
    25261 acgagcagcc atgtccacct gacgagcttc ccccacttcc ctgatcaagt cagaaatcag
    25321 ctcaacgtaa tcatcaataa gctcgcgccg atgcgcctct ctgacctggc ggaacccttc
    25381 aacgtgctct tccacgttca ctaactgcgt cacttttttt gctgttggcg tacctgcgcg
    25441 acgactcatt gtgcttcctc attatggtga cgcatacata gcgttctcat ttcgtgcgca
    25501 cattgtaaac cagagttgcg aaggtacaaa aaattaacgt tttagcaata gctacataat
    25561 atagcctgtg ctatatctgt atgtaatgca atcatccctc aaggatcgac gggattagca
    25621 agtcaggagg tcttatgaat gagttcaaga agtgtatgcg cgtgtttagt cattctccct
    25681 ttaaagtacg gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac aaaccgcagc
    25741 aagataaacc ttccgataaa tagcggcgtc gcggtacgcc gcttcactcc tgcttttatg
    25801 caggcataac gcgttttggt ctgaaaaacc ccactttttg tcggatttgc aatccccttc
    25861 gcaaaagatt tgttcgtcag tagttgacct gaacggcggc tcgctctatc ttcttgcagc
    25921 cctacgtata ttgcggctcg cggatgcgga cccctttccc ctcttcacgc actcttgcag
    25981 gtattgaccc ttgacgccag ggtaagcaca tggcgtttgt tacgatagtg gcatatgaat
    26041 ttaaccctca aagaatcgct tgttacccgt agccgggtat ttagcccgtg gactgcgttc
    26101 tactttttac agtcgctatt aattaacctc ggcttaggtt accccttcag tttgctctac
    26161 accgctgcgt ttacggctat tttgcttttg ctatggcgaa cattgcctcg cgtacaaaaa
    26221 gttctggtcg gtgtcagttc gctggtggcg gcttgttatt tcccttttgc tcaggcctac
    26281 ggcgcgccta atttcaatac attgctggca ttgcactcca ccaatatgga agagtcgacc
    26341 gaaatcctga cgatttttcc gtggtacagc tacctggtcg gcttatttat ttttgcgctc
    26401 ggcgtaatag caatcaggcg aaaaaaagag aatgaaaaag cgcgctggaa taccttcgac
    26461 agcctgtgtc tggtattcag tgtggcgaca ttttttgttg ctcccgtgca aaacctggcc
    26521 tggggtggcg tatttaaact gaaagatact ggctatccgg tatttcgttt tgctaaggat
    26581 gtcatcgtca ataataacga ggtgattgaa gagcaagaac ggatggcaaa actttccgga
    26641 atgaaagata cctggacggt cactgccgtt aagccgaagt atcagaccta tgtggtggtg
    26701 atcggcgaaa gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg ggacaatacc
    26761 ccgtttgcca gcagcgttaa cggtttgata tttgctgact acattgccgc cagtggctcc
    26821 acgcagaaat cacttggctt aacgctcaat cgcgtagtcg atggcaaacc acagtttcag
    26881 gataactttg tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt ttccaaccag
    26941 ggccaaatcg gcgaatacga tactgctatc gccagcatcg ccaaacgtgc agatgaagtg
    27001 tacttcctga aagaaggtaa tttcgaagca gataaaaaca cgaaagacga agcgttactg
    27061 gatatgaccg ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat tgttctgcat
    27121 ctgatgggct cgcatccgca ggcctgcgac aggacacaag gcaaatacga aacctttgtg
    27181 caatcgaaag aaacgtcgtg ctatctctat accatgacgc aaacggacga tttactgcgc
    27241 aagctgtacg atcagttacg caacagcggc agcagcttct ctctggttta cttttctgac
    27301 cacggtctgg cctttaaaga gcgcggcaaa gacgtgcaat accttgccca tgatgataaa
    27361 tatcagcaaa atttccaggt gccttttatg gtcatttcca gcgacgataa agcgcatcgg
    27421 gtgattaaag cccgccgctc agccaatgac ttcttaggct ttttctcgca gtggacggga
    27481 attaaagcga aggaaataaa tatcaaatac ccgtttatat ctgagaagaa agccgggccg
    27541 atatacatca ccaacttcca gttacagaag gtggattaca accatctcgg aaccgatatt
    27601 ttcgacccga aaccttaaaa caaaaaatcc gccccgagag gcggattttt tatatcacca
    27661 aagtgattag aagcggtaac caacaccggc aatccaggtg cctacgtcaa cgctacgaat
    27721 acggctctgc tcgtaagaga agtccagagc aacgttttcc atcgggttga actgcagacc
    27781 agcaccgtag gagaaaccgt agtcgctggt gtcgtgtttg taggtcgggt attcagtggt
    27841 ctggaattta ccataaccca cccccactac accgtagatg cttgcccagt cgttaatgcg
    27901 gtaagccgga ccagcagtga tgccgtagta ctggtttttg ttgtagtcac cagagcttgc
    27961 agtacggctt ttctcggtgt aagtgaaaga accgatcaca cccagcgggc tgttgtcttc
    28021 ttcatagcgg tatttcaggt tgaaaccgcc cattttgttc atttggccct gagcgtcgct
    28081 ctgtgcgtaa ccgccagtta cggtagaagt cgcagctacg gaagtacctg cggtgaaagc
    28141 cagaactgcg gccagtgctg aaagacatgc aatttttttc ataaccacct caaatgtgat
    28201 tcaaataagt cctaagtttt aaatatatca aaaattaatg ggaaactctt cgcgatttgt
    28261 gatgtctaac gggccatttc atgtaacaga acgtttccat acaccgctat ccatctaaat
    28321 ttaaatcact ttttcagaga actgcgtaag tattacgcat gttttccctg tcattcatcc
    28381 agattattcc taatcaccag actaatgatt ccatcaatcc tggcgcattt tagtcaaaac
    28441 gggggaaaat tttttcaaca aatgctcaac cagcattggg tatatccagt acactccacg
    28501 ctttacttaa gtctagatat ttgtgggaga aaggatgcct ggttcattac gtaaaatgcc
    28561 ggtctggtta ccaatagtca tattgctcgt tgccatggcg tctattcagg gtggagcctc
    28621 gttagctaag tcactttttc ctctggtggg cgcaccgggt gtcactgcgc tgcgtctggc
    28681 attaggaacg ctgatcctca tcgcgttctt taagccatgg cgactgcgct ttgccaaaga
    28741 gcaacggtta ccgctgttgt tttacggcgt ttcgctgggt gggatgaatt atctttttta
    28801 tctttctatt cagacagtac cgctgggtat tgcggtggcg ctggagttca ccggaccact
    28861 ggcggtggcg ctgttctctt ctcgtcgccc ggtagatttc gtctgggttg tgctggcggt
    28921 tcttggtctg tggttcctgc taccgctggg gcaagacgtt tcccatgtcg atttaaccgg
    28981 ctgtgcgctg gcactggggg ccggggcttg ttgggctatt tacattttaa gtgggcaacg
    29041 cgcaggagcg gaacatggcc ctgcgacggt ggcaattggt tcgttgattg cagcgttaat
    29101 tttcgtgcca attggagcgc ttcaggctgg tgaagcactc tggcactggt cggttattcc
    29161 attgggtctg gctgtcgcta ttctctcgac cgctctgcct tattcgctgg aaatgattgc
    29221 cctcacccgt ttgccaacac ggacatttgg tacgctgatg agcatggaac cggcgctggc
    29281 tgccgtttcc gggatgattt tcctcggaga aacactgaca cccatacagc tgctggcgct
    29341 cggcgctatc atcgccgctt caatggggtc tacgctgaca gtacgcaaag agagcaaaat
    29401 aaaagaatta gacattaat
//
LOCUS       DAZPAC010000033        29165 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZPAC010000033 DAZPAC010000000
VERSION     DAZPAC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29165
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.033"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHUPM_001181"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHUPM_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR0869024.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="ACHUPM_001182"
     tRNA            1230..1305
                     /locus_tag="ACHUPM_001182"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="ACHUPM_001183"
                     /pseudo
     CDS             complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="ACHUPM_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="ACHUPM_001184"
     CDS             complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="ACHUPM_001184"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR0869025.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="ACHUPM_001185"
     CDS             complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="ACHUPM_001185"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR0869026.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="ACHUPM_001186"
     CDS             complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="ACHUPM_001186"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR0869027.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4869..5306)
                     /locus_tag="ACHUPM_001187"
     CDS             complement(4869..5306)
                     /locus_tag="ACHUPM_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFR0869028.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5524..5699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5788..6464)
                     /locus_tag="ACHUPM_001188"
                     /pseudo
     CDS             complement(5788..6464)
                     /locus_tag="ACHUPM_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="ACHUPM_001189"
     CDS             complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="ACHUPM_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFR0869029.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7988..8272
                     /gene="pduA"
                     /locus_tag="ACHUPM_001190"
     CDS             7988..8272
                     /gene="pduA"
                     /locus_tag="ACHUPM_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFR0869030.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8269..9081
                     /gene="pduB"
                     /locus_tag="ACHUPM_001191"
     CDS             8269..9081
                     /gene="pduB"
                     /locus_tag="ACHUPM_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFR0869031.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            9100..10764
                     /gene="pduC"
                     /locus_tag="ACHUPM_001192"
     CDS             9100..10764
                     /gene="pduC"
                     /locus_tag="ACHUPM_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFR0869032.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10774..11442
                     /locus_tag="ACHUPM_001193"
     CDS             10774..11442
                     /locus_tag="ACHUPM_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFR0869033.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11457..11975
                     /gene="pduE"
                     /locus_tag="ACHUPM_001194"
     CDS             11457..11975
                     /gene="pduE"
                     /locus_tag="ACHUPM_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFR0869034.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11985..13817
                     /locus_tag="ACHUPM_001195"
     CDS             11985..13817
                     /locus_tag="ACHUPM_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFR0869035.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13807..14157
                     /locus_tag="ACHUPM_001196"
     CDS             13807..14157
                     /locus_tag="ACHUPM_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFR0869036.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14177..14452
                     /gene="pduJ"
                     /locus_tag="ACHUPM_001197"
     CDS             14177..14452
                     /gene="pduJ"
                     /locus_tag="ACHUPM_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFR0869037.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14476..14898
                     /locus_tag="ACHUPM_001198"
     CDS             14476..14898
                     /locus_tag="ACHUPM_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFR0869038.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14901..15533
                     /locus_tag="ACHUPM_001199"
     CDS             14901..15533
                     /locus_tag="ACHUPM_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFR0869039.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15530..16021
                     /gene="pduM"
                     /locus_tag="ACHUPM_001200"
     CDS             15530..16021
                     /gene="pduM"
                     /locus_tag="ACHUPM_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFR0869040.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16025..16300
                     /gene="pduN"
                     /locus_tag="ACHUPM_001201"
     CDS             16025..16300
                     /gene="pduN"
                     /locus_tag="ACHUPM_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFR0869041.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16312..17319
                     /gene="pduO"
                     /locus_tag="ACHUPM_001202"
     CDS             16312..17319
                     /gene="pduO"
                     /locus_tag="ACHUPM_001202"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFR0869042.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            17316..18701
                     /gene="pduP"
                     /locus_tag="ACHUPM_001203"
                     /pseudo
     CDS             17316..18701
                     /gene="pduP"
                     /locus_tag="ACHUPM_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18712..19824
                     /locus_tag="ACHUPM_001204"
     CDS             18712..19824
                     /locus_tag="ACHUPM_001204"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFR0869043.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            19821..21166
                     /locus_tag="ACHUPM_001205"
                     /pseudo
     CDS             19821..21166
                     /locus_tag="ACHUPM_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            21169..21723
                     /gene="pduT"
                     /locus_tag="ACHUPM_001206"
     CDS             21169..21723
                     /gene="pduT"
                     /locus_tag="ACHUPM_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFR0869044.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21723..22073
                     /gene="pduU"
                     /locus_tag="ACHUPM_001207"
     CDS             21723..22073
                     /gene="pduU"
                     /locus_tag="ACHUPM_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFR0869045.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22078..22521
                     /locus_tag="ACHUPM_001208"
     CDS             22078..22521
                     /locus_tag="ACHUPM_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR0869046.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22622..22951)
                     /locus_tag="ACHUPM_001209"
     CDS             complement(22622..22951)
                     /locus_tag="ACHUPM_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR0869047.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23123..24181)
                     /locus_tag="ACHUPM_001210"
     CDS             complement(23123..24181)
                     /locus_tag="ACHUPM_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR0869048.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24379..24852)
                     /gene="sbmC"
                     /locus_tag="ACHUPM_001211"
     CDS             complement(24379..24852)
                     /gene="sbmC"
                     /locus_tag="ACHUPM_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFR0869049.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(24971..26137)
                     /gene="dacD"
                     /locus_tag="ACHUPM_001212"
     CDS             complement(24971..26137)
                     /gene="dacD"
                     /locus_tag="ACHUPM_001212"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFR0869050.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26346..27773
                     /gene="sbcB"
                     /locus_tag="ACHUPM_001213"
     CDS             26346..27773
                     /gene="sbcB"
                     /locus_tag="ACHUPM_001213"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR0869051.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27816..28043)
                     /gene="tsuB"
                     /locus_tag="ACHUPM_001214"
     CDS             complement(27816..28043)
                     /gene="tsuB"
                     /locus_tag="ACHUPM_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR0869052.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28057..29115)
                     /gene="tsuA"
                     /locus_tag="ACHUPM_001215"
     CDS             complement(28057..29115)
                     /gene="tsuA"
                     /locus_tag="ACHUPM_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR0869053.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
BASE COUNT         7707 a         7640 c         6944 g         6874 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaaacat cagcgcccgg gttcgcttcc
     2101 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     2161 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     2221 ggataagtta ccgccaggct attatggctg gcaaaaagca gagcaaatga gcaaaaaata
     2281 tttacacgac gcatcatgtc cctttcctaa gtcgcgaaag ctatccgtta agtatagctt
     2341 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     2401 attactggca gcaagttcgc ccatgttgtt atatatcgca caggcagctt cgatgatggg
     2461 catcgccaga gctgcgccac taccctcacc taaacgcatc tccatattga gataaggctc
     2521 cagccccaaa tgcgacagcg caatacgcgc ccctttttct gccgacaggt gagaaggaat
     2581 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcggcag cataagaaag
     2641 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     2701 tccgaccaaa tcaaatccac ccacttttgc caggacatca acgccatcct gcggatttgg
     2761 ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     2821 caggtttgcg ccaatcccaa ccacttcttc aggagcccgg ccagtgattg tgctgactat
     2881 tgccgctgcc ggtgtcgtgt ttgccatccc caggtcacct acaccaaaca gcgtaacacc
     2941 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt ctgccagatg
     3001 gcgactcatt gccggagctg aagcaatatt gccactacct cgagcaacac gcatgttgat
     3061 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtaga cgttagcgcc
     3121 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgaat
     3181 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     3241 catgaccagt accgcttttt tgcccacatg cggaacgcca ttcaaccccg gcattcctgc
     3301 cagttgtatg gcaagcgcct ccagccttcc caggctacca acaggtttga gtaacccgtc
     3361 gatatgccgt tgtgcacgcg acatagcggc aggatcaata gcagggatcg tattcagtaa
     3421 atcggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa gaccagttca
     3481 ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     3541 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     3601 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     3661 ccatcaattt tgccaatgaa cacattgcca agtccttctt cccgggcata acgatgacga
     3721 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     3781 atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag tacaacaaaa
     3841 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     3901 tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc caggtggaat
     3961 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     4021 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaattaaa
     4081 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     4141 ggtacaggca gacgcgtaat aaaagagagc atcgcccaaa ataatttact catttgattt
     4201 ttactccaat acccgaaacc accaaccata cttcatttgc cgcagcggcc agctgctgat
     4261 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     4321 ttcccacttc gttagtcact aatacaacct ttgcgggaca acgttggcag gcagcaatca
     4381 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     4441 ctttatcgcc gccataatca aacaacagat tagtcaccat ggtggtaacg cattccagca
     4501 atacagcctc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct
     4561 ccactgtgtg ccagtgcgcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     4621 catcatcaag gatttgtgag gtggcgatat acagaacctg cgaagagtcc ccaataagcg
     4681 cctctgcatg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     4741 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     4801 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     4861 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     4921 cgacaaaatg cgtcattgac agcggtttct ctggaatgac agatttcatt cagataatgg
     4981 attaatgcta cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     5041 aaaattaccg tcgttccctc agaatgagcg agccattctg gtacggatat ctcgctttca
     5101 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataacga ttgcacaggc
     5161 ccaccatcaa gaggctggaa atcttgctgc cagtcctcac caacaaaaca ggttattggc
     5221 aatatttcag ccagatgttt cgccagatcc acatctccag ataagaggca aaaaagagct
     5281 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttaatttc ctgtaatggc
     5341 ggtctggttg ataagccatg aaacaatatt gccacacctt gaatgcagcc ctggtgcctg
     5401 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     5461 ggggtataaa gacgcgccag tactctaccg gacgcttccg ggcaacccac ttcccaccga
     5521 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc acccgacctt
     5581 cccaatctcg cgatcagtgg cattttcggg tttgtcagca ttacagcagc gggggctgcg
     5641 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     5701 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgtttattt
     5761 aaaccttccc tttttttagc atttattcta taccgtttcc atcgccgcat tttcactcac
     5821 ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt tgcaaaaata
     5881 actgtattgt cgtggggatt aggcgcacga gtctggcgca ccgaatctga ccgagatttg
     5941 ttctctttaa taaccacaaa attgagttgt tttttgaggc aattttccac aatcaacttc
     6001 aacaaatcag cagaggccat aactcttgag taatccatct caggaacttt gcggaattca
     6061 ttaagtaatt cgggatctgc ctgccagcaa tcatcgacat tcagaatatc gaccaaatcg
     6121 acatcatcat tacgcagacg aacttgcccg cacaatacaa aaccgactaa atgtccggcg
     6181 attaccagtg gaatagagaa atcggttaat ccggcatgac agcgataaat acaaggctga
     6241 tcggatttag aggcttccag gcccccacaa cgatcgctca tgcgacatcg agtgcttagc
     6301 tgagggtgtt gccgcatcat ctgacaaaat ggagtgaagt taaacagctc ggaaatttca
     6361 tcgccgtgaa tattgaccac gacaaccgca aggctggtcg cctgcgcaaa atcctgcgcg
     6421 attttattaa tgagttctga attaagtgtc gccgcagaaa tcatgataaa acctcccagt
     6481 taatttattg ttatagcaaa acaattgttt tacatttaat cttcacatca tccctgatga
     6541 tttactgacg agaagcacaa aggacaataa caaaagcctc cgtgaagcgg gagacttttt
     6601 aataagttcg gaaagcagat cacaaacagt aaaaaaataa atgtttataa ctgccagagt
     6661 atttcgttat tcgctaattc ctttaggtgt aacactattg acaggtaaat tctttgcaat
     6721 gagatagcga taaatcgccg caccaacaca tgccccaatg atcggtgcaa taatgggtac
     6781 aatgaaataa ggaatattgc gtccaccagt catcgcaatt tctccccacc ccgcaagcca
     6841 ggtgaaaatc ttaggtccaa aatcacgcgc tggattcatg gcaaaaccgg ttaatggtcc
     6901 ggtagaagca ccaataacag caaccagaat accaataagt aaaggtgcaa gcggtccttt
     6961 gggtacgcca ttaccgtcat cggtcaacgc cataatcaac cccattaaca tggacgttat
     7021 gaccacttcg accaacgcag cttgccatac attaatcgcc gctgccggat aagtactgaa
     7081 gatactggcg agttgtaaac tttctgcgct accacgcacc atattgtgag cagattcaaa
     7141 atcggtaaac agactactgt atagagaata tgccagaact gcaccaccaa aggcacccgc
     7201 taattgcgcc agacaaaatg gcagcacttt acgggctggg aaagaggcaa acagccacag
     7261 agcaatggta actgccgggt tgagatgagc gccggaaata cctgccgtaa ggtaaacagc
     7321 aagcgaaatc cctaaccccc aaatgataca aatttcccac agtcccagac ttgccccggc
     7381 aactttcagg gcgcacaaac aaccaatgcc aaaaaagagg aacagtccgg tgcccaaaaa
     7441 ctcggcaacg cattgcgcct tgagtgaatc attcatagtg acaccttttt tacggagttt
     7501 ttctttatca gccaactatg cgctgactgt tgtatatacc gagcacaact ataaaaaatt
     7561 gctctgcaga aaatattgcc aacatgcatg acagactcgt gtaaaaatcg tgctaacata
     7621 acaaattatt gtcattaccg aatcatgcaa cacagcgtta gtcagtgttg aaagtaataa
     7681 ttcccaccaa acttttacaa taaaaaattg ccatcatgac ataataaaat tgaaacaaat
     7741 atcacagttc cttcttcgaa ctaaactcaa ccaaatatta ctcgcgatta aactcactag
     7801 ccaacgcaat taattcacat tgaaatatcc acaaaacaac caataaatta acaacatata
     7861 gttcataaaa gcaattttat aatatttgtt ctctattaat ttctctcatt agccgattaa
     7921 gtcagccatt ctttttcctc gctttttttt atagtcatat cgtcaaaaaa atccaggcga
     7981 ggtctttatg caacaagaag cgttaggaat ggtagaaacc aaaggcttaa ctgccgccat
     8041 agaggccgca gacgccatgg tgaagtcggc aaatgttttg ctggtcggct acgaaaaaat
     8101 aggttccggg ctggtgaccg tcatcgttcg tggtgatgtc ggggcggtaa aagcggcaac
     8161 agacgcgggt gccgcagctg cgcataacgt cggcgaagta aaagccgtac acgttattcc
     8221 ccgcccacat accgatgtcg aaaaaatctt accaaaggga attaacccat gagcagcaat
     8281 gaactggtcg aacagatcat ggcgcaggtc atcgctcgcg tagcgacgcc agagcatgag
     8341 gttctcccga aaaccactca tcaaatacga gagacggcta tggcagaaaa aagctgcacg
     8401 ctaacagaat ttgttggcac cgctattggc gacactgtag gtctggtcat tgcgaacgtt
     8461 gatagcgccc tgttggacgc gatgaaactt gaaaagcgct atcgttccat tggcattctg
     8521 ggtgcgcgta ttggcgcagg gccacacatt atggccgctg atgaagcggt aaaagccacc
     8581 aacactgaag ttattagcat tgaacttcct cgcgatacta aaggcggtgc cggacacggc
     8641 tcactgatcc tcttaggtgg caacgatgtt tccgacgtga aacgcggtat tgaagtcgcc
     8701 ctgaaagaac tcgatcggac ctttggcgat gtctatgcca acgaagccgg acatcttgaa
     8761 ctgcaatata ccgcccgcgc cagctatgca ctggaaaaag cgtttggtgc accactggga
     8821 cgggcttgcg gcgttattgt cggcgctccg gctgctgtgg gtgttctgat ggccgacacc
     8881 gcgctgaagt ccgccaatgt ggatgttgtg gcatacagct ccccagctca agacaccagc
     8941 tacagcaatg aagtcattct gattatctcg ggtgactccg gcgcagtccg ccaggcggtc
     9001 atctccgcac gcgaaatcgg taaaaccgtg ctttcgactc tcggcgatac accgaaaaac
     9061 gatcaccctt cctacatctg aattctctga ggcagattta tgagatcgaa aagatttgaa
     9121 gcactggcaa aacgcccggt caaccaggac ggtttcgtta aagagtggat tgaagaaggc
     9181 tttattgcga tggaaagccc gaacgatcct aaaccgtcaa tcaaaattgt taacggcgtc
     9241 gttaccgaac tggatggaaa acccgccagc cagtttgacc tgatcgacca ctttatcgcc
     9301 cgctacggca tcaatctcgc gcgcgccgaa gaagtcatcg cgatggattc ggtaaaactt
     9361 gccaatatgt tgtgcgatcc aaatgtcaaa cgtagcgata tcgttcccct taccacagca
     9421 atgacgcctg caaaaatcgt cgaagtggtt tcgcagatga acgtggtaga gatgatgatg
     9481 gcgatgcaga aaatgcgcgc ccgtcgaaca ccatcacaac aggctcacgt caccaacgtc
     9541 aaagataacc cagtacaaat tgccgccgac gcagctgaag gcgcatggcg cgggtttgat
     9601 gaacaggaaa ccaccgttgc cgttgctcgc tatgcaccgt tcaacgccat cgccctgctg
     9661 gtcggctcac aggtgggacg tccgggcgtc ttgacccagt gttcactgga agaagccacc
     9721 gaactgaaac tcggtatgtt aggccacacc tgttacgccg aaacaatttc tgtctatggt
     9781 acagaacctg ttttcaccaa tggtgatgat accccgtggt caaaagggtt tctcgcgtca
     9841 tcctatgctt ctcgtggctt aaaaatgcgc tttacttccg gctccggctc tgaagtacag
     9901 atgggttacg cggaaggcaa atcaatgctt tacctcgagg ctcgttgcat ttacatcacc
     9961 aaagccgcag gcgttcaggg tctgcagaat ggttcggtaa gctgcatcgg tgttccatca
    10021 gctgtgccat caggcatccg cgcagtatta gcggaaaact taatttgctc atcgctggat
    10081 ctggaatgcg cctccagtaa tgaccagacc tttacccact cggatatgcg ccgtaccgca
    10141 cgtttcctga tgcaatttct gccaggcacc gactttatct cttccggttt ttccgctgtg
    10201 ccgaactacg acaacatgtt tgctggttca aacgaagatg ctgaagactt cgatgattac
    10261 aacgtcattc agcgtgacct gaaggttgat ggtgggctgc gaccagtacg cgaagaagat
    10321 gttatcgcta tccgcaataa agccgctcgc gctttacagg ctgtctttgc cggtatggga
    10381 ctaccgccta ttaccgatga agaagtcgaa gcggcaacct atgcccacgg ttcaaaagat
    10441 atgcctgagc gcaatattgt ggaagacatc aagttcgccc aggagattat caataaaaac
    10501 cggaacagcc tggaagtggt gaaagccttg gcgcaaggca gttttaccga tgtcgcccag
    10561 gacatgctca acatgcaaaa agcaaaactg accggcgatt atctccatac ctcagccatc
    10621 atcgtcgatg acggacaagt gctctctgcg gtcaatgacg tcaatgatta cgccggaccg
    10681 gctacaggtt accgcctgca aggggaacgc tgggaagaga tcaaaaatat tcccggtgca
    10741 cttgatccca acgaaattga ctaagggtga gaaatggaaa ttaacgaaaa attactgcgt
    10801 cagattatcg aagacgtgct ggcagaaatg caacccagcg acaagtccgt ttcatttcgc
    10861 gcacctgtct ctgcaactgt cccttccgcg ccagataccg gaaattttct gaccgaaatt
    10921 ggcgaagccc aacaaggcac tcagcaagat gaagtcatta ttgctgtagg cccggcgttc
    10981 ggcctggcgc aaaccgtcaa tatcatcggt attccgcata aaaacatctt gcgcgaagtg
    11041 attgccggta tcgaagaaga aggcattaaa gcccgtgtaa ttcgctgctt taaatcgtcc
    11101 gatgtcgcat tcgtagcagt cgaaggcgat cgtcttagtg gttcaggtat tgctatcggt
    11161 attcagtcga aaggcacaac ggttatccac cagcagggac ttcctccctt gtctaacctg
    11221 gaacttttcc cacaagcgcc tttgttgacg ctggatactt atcgtcagat tggcaaaaac
    11281 gccgcgcgtt atgcgaagcg cgagtctcca caaccggtgc cgaccctcaa cgatcaaatg
    11341 gcgcggccga aataccaggc caaatctgcc attttgcata ttaaagagac gaagtacgtg
    11401 gtcactggca aaaaaccgca agaactgcgc gtgacgtttt aacaaaggaa accttcatga
    11461 ataccgatgc aattgagtcg atggtcaggg acgtgttgaa ccgcatgaac agtttacagg
    11521 atgcagctcc tgtttctgcg gtccccaacg catcaatact gagcgccaaa gtgacggatt
    11581 acccgctggc aaataaacat ccggaatggg ttaaaacagc gaccaataaa acgctcgacg
    11641 atttcactct ggaaaatgtg ctaagcgata acgtcaccgc cctggatatg cgcattaccc
    11701 ctgaaacatt gcgtattcag gctgcgattg ccagagatgc tggtcgcgat cgtctggcga
    11761 tgaattttga acgcgccgct gagcttacct cagtaccaga tgatcgcatc ctcgaaatct
    11821 acaatgcgtt acgcccttat cgttcaacta agcaagaact tattgccatt gctgacgatc
    11881 ttgaacagcg ttatcaggcc aaaatttgtg ctgcatttgt ccgtgaagct gcggaacttt
    11941 atgtcgagcg caaaaaactg aaaggcgacg attaaggacg aactatgcga tatatagcag
    12001 gcattgatat cggcaactca tcaacagagg ttgcgctggc aacgctcagt gcaactggag
    12061 agttgtcgtt cgtgagtagc gccctggagg aaaccaccgg aatcaaaggt acgttgcgta
    12121 acgtccatgg tattcaggag gcgctcgcac aggcaacgaa aaaagtcggc atcaatgtta
    12181 gcgatatttc gcttattcgt atcaacgaag caactccggt cattggtgat gtggcgatgg
    12241 aaaccatcac tgaaactatc atcaccgaat caaccatgat cggtcacaac ccgaaaactc
    12301 cgggcggagt ggggctgggc gtagggttca ccatcactcc gcaagagtta ttaacacgcc
    12361 cggcggacac accttacatc ctggtggtgt catcggcatt tgattttgcc gatatcgcga
    12421 cgatgataaa cgcctccgta cgcgccggat atcagctgac tggcgttatt ctgcaacgcg
    12481 acgatggcgt actggtcaat aaccgcctgg aaattccttt acctattgtg gatgaagtcc
    12541 tctacatcga tcgtattccg ctcggcatgc tggcagcaat tgaagttgcc gttcccggga
    12601 aagttatcga aaccctgtct aacccgtatg ggattgccac cgtattcgcc ctcaatgctg
    12661 aagagactaa aaatattgtt cctgtcgcgc gggcgctaat cggcaaccgt tcagcagtgg
    12721 tagtgaaaac gccctctggc gacgttaaag cccgttcgat acccgccgga aatatcgaac
    12781 tgctttctgc cgggcgcaca acgcgcgttg atgtagctgc tggtgccgat gccattatga
    12841 aagcggtcgg tgagtgccca aaactggaaa acgtgacggg agaaccaggc accaacatcg
    12901 gcggaatgct ggaacatgtt cgccagacca tggcggaact gaccaacaaa cccagcaacg
    12961 aaatttttat tcaggatttg ctggcgatag atacctctgt ccccgtcagc gtaaccggtg
    13021 gtctggcagg tgaattttca ctggaacagg ctgtgggtat tgcctctatg gtgaaatccg
    13081 accgtctgca aatggcgatg atcgccagtg aaattaagca aaaactgcat gttgatgttc
    13141 aggtcggagg cgcagaagca gaagccgcca ttcagggggc cttgactacc cctggaacca
    13201 cacgtccact ggcaattctg gacttaggtg caggctctac cgatgcctcc atcatcaatc
    13261 agagtgggga aatcgttgcc actcaccttg cgggagcggg cgacatggta acgatgatta
    13321 tcgctcgtga gctgggctta aatgatcgtt atctggcaga agaaatcaaa aagtacccgt
    13381 tagcaaaagt agaaagcctg ttccacttac gccatgaaga cggtagcgtt cagttcttcc
    13441 ctactcccct atccccccat gtatttgccc gcgtctgtgt agtgaaaccc gacgaactgg
    13501 tgcctattcc cggtgatttg acgctggaaa aagtacgcgc tgttcgtcgc agtgcgaaag
    13561 agcgtgtctt cgtaaccaat gccctgcgcg cgctacgtca ggtaagccct gccggaaata
    13621 ttcgcgacat tccgttcgtc gttctggtgg gtggttcttc gctggatttc gaagtaccgc
    13681 aattggtcac cgacgcgctg gcacattatc gactggtcgc cgggcggggg aatatccgtg
    13741 gtagcgaagg tccgcgcaac gcggtagcta ctgggctact catcgcctgg cacaaggagt
    13801 cgattcatgg aaagtaaaga gcgtgcacca gccatcgttg tcatggagat cggtgactgt
    13861 atcactacat gggatgaggt gcttcttggc attgaagagg aaggtattcc ctttcgcatt
    13921 caacacattc cgtcaggtga agttatcgat agcgcctggc tggctgctcg tcaatctccc
    13981 ctgttggttg gcattgcctg cgaccgggaa gaactgattg tgcattacaa aaacttaccg
    14041 gcatcagcgc cactgtttac gctgacgtat caacaagata accatacccg gcgcagcatc
    14101 ggcactaacg ctgcccgcct ggtaaaagga attcccttcc gggaattcca ctcataatcc
    14161 acaggagaaa cgctgtatga acaacgcact aggactggtt gaaacaaaag ggttagttgg
    14221 cgcaattgaa gctgcagatg ccatggttaa atccgccaac gtgcagctgg ttggctatga
    14281 aaagattggt tcgggtctga ttacggtgat ggtccgcggt gatgttggcg cagtaaaggc
    14341 cgccgttgat gcggggagcg ctgcggcaag cgccgttggc gaagtgaaat cctgccatgt
    14401 catcccgcgc ccacacagtg atgttgaggc tattttacct aaatcggcat aaccccattt
    14461 gctaaggagc acagcgtgaa gcaatcactg ggattacttg aagccagtgg tctggctctc
    14521 gccatcagtt gcgcagacgc tatggcaaaa gccgcgtcca tcacgctggt tggccttgag
    14581 aagaccaatg gctcaggctg gacgctcatc aaaatcaccg gtgacgtcgc ctcggttaat
    14641 gcggcgatcg cgacgggagc cagtttcgcc ggacagcgaa atggccttgt cgcacacaaa
    14701 gtgattgccc gcccgggaga cggcattctg ccccacacaa ctgaagcatt gaccgtgacg
    14761 gttgaaacaa gcaaaacacc agcaacaacc gtcacctcag agattatcag ttgtaatttg
    14821 tgtcttgacc cggcatgtca gcggcaaaaa ggtgagcccc gctcactttg cctgtacgcc
    14881 aataagcgag gggagtagca gtggataaac aaaccttgca ggaaacagtg agccagattc
    14941 tggatgagat gcgcgagcgc cctatcccac tgggcgtttc cagtcgccat atacatcttt
    15001 catcagcgga ttatttgcgt ctttttcctg aacaaccgat tagcgagaaa aagccgttaa
    15061 tgcaacctgg ccaatacgct gctgaacagg ttgttacgct ggagggacca aaaggcaaat
    15121 tgcacaacgt tcgcttgttg gggccgctac gcaatgcaag ccaggtggaa atttcccgga
    15181 cggacgccag aacgttaggg atcaccgccc cgctacgcat gtcaggcgat ctcagcggca
    15241 ctccggggat tcgtttgata agcccatttg cggaactgga gatcacatct ggcgtcattg
    15301 tcgcgcaacg gcatattcat atgtctccgc tggatgccct gatttatggc gttacgcatg
    15361 gcgataaagt ggcggttgcc attgaaggtg atgagcgacg actgatattc gaaaacgtcg
    15421 ctgttcgcgt ctctccggat atgcgtcttg aaatgcatat tgataccgat gaggccaacg
    15481 ccgcaggcgc tgatagccct gacgtttttg ccacactggt gtcagtgcca tgaataacat
    15541 gctgatgcaa cgtattgttg acgaagtggt ttttcgcctt aaacaacgtg cgggaaaaac
    15601 actcgtgctg actgtttttc aattgcgaga cgccagcgtt caagagagcg ttcatcagta
    15661 tgcgtcatta caaatccgat atgtcgatct gccgctgctg cgacaacttg cggagaacga
    15721 aacgtcagat cgagcatcta tccagataca cgaagcgtta gcctggggaa tgcacattca
    15781 attgtcgttg cagcgtcatt tcctgaacgc catcgaattg aagactctcg cccgtctacc
    15841 gctaagctgg tgcgacgaac aagggcagcc gatctacctt catcataatc gacttttaag
    15901 ttatgccgat atcgcacagt taagctcggg aatactggtg ttacagcgga aatgttgtgt
    15961 gaccgcactg gctcgtgaag cggcaattac ccgaaatatt cagttaatca ggcaggagta
    16021 acctatgcat ctggctcgag tcactggcgc ggtagtatcc acgcaaaaat caccttctct
    16081 gatcgggaaa aaactgctgc tggttcgccg ggtcagtgct gacggcgaac tgcctctcac
    16141 gccattaacc ggcgacgaag ttgccgttga ttcagtcggc gcaggcgttg gtgagttggt
    16201 actcctcagc agtggttcca gcgccagaca tgtgttttca ggccctaacg aggccatcga
    16261 cctggcagtg gtcggcattg tcgatacgct ttcgcgctaa ggagctaaaa gatggcaatt
    16321 tatacccgaa caggtgattc aggaagtacg tctttattta ccggacagcg cgtcagcaaa
    16381 actcatctac gtgtcgaaac ctatggcacg ctggatgaac tgaacgcgac actcagtctg
    16441 tgttattgcg ccaccgccat cgaaagccat cgcatcttac tggaagccat tcaacaacag
    16501 atattttggt ttagcgccga actcgccagt gaaagcgaac agccgtcagc gcaacagcgt
    16561 tacatcggta cggaagaaat tgccgcgttg gaaaacgcga tcgatagtgc aatgaacgcc
    16621 gtccccccag ttcacagctt cattctgcct ggtcgatgtg aagccgcaag ccgcatgcat
    16681 ttcgcccgca cggtggctcg acgggctgaa agacggctgg ttgaactgac aacggaaacc
    16741 accgtccgga atgttttgct gcactatatc aaccgacttt ccgattgcct gtatgccctc
    16801 gcccgcgtgg aagacaacgt tgctcatcaa aacctgatga ttcaggaaat cacaaaacgt
    16861 tatcacgaag ccaaccacac accagcattg aaggaacgca caatgccgct cacttttcag
    16921 gatcttcacc agctcattcg tagtgcggca atgcgcgcgg atgaactgca tattcccgtt
    16981 gtcatcagca ttgttgatgc caacggaaca gaaagtgtta cctggcggat gcctgatgcc
    17041 ctgttggtca gcagtgaact ggcaccgaaa aaagcctgga ccgccgtggc gatgaaaacg
    17101 gcaacccaca agcttgctga taccgtgcaa cccgacgcgc cgctttacgg gctcgaaagc
    17161 catatgcaag gaaaagtcgt cacttttggt ggcggcttcc ccctctggcg tgacggaaaa
    17221 ttgcttggcg ggcttggcat cagcggcggt agcgttgaac aagacatgga tattgcccaa
    17281 agcgcaatgg cggcaattaa cgtgggagta aaccaatgaa taactctgaa ttagaaacac
    17341 tgatccgcaa tattctgcgt gagcaacttg tccccgctac accacgaacg caaagaaacg
    17401 ctattttcca gactgtcgat gaagcggtct gcgcagcaca ctaggccttt ttacgctttc
    17461 aacagtgccc gcttaaaacc cgtagcgcca ttatcagcgc cattcgcgaa gaattaatgc
    17521 cccgccttac cgaactggca gaagaaagcg ccaaagaaac ggggatggga aataaagaag
    17581 ataagtacct aaaaaacaaa gcggcactcg ataacacgcc aggaattgaa gatttaacca
    17641 ccacagcact aaccggtgat ggtggcatgg ttttgtttga gtactctccg tttggggtga
    17701 ttggctcggt cacgccaagc accaacccca cggaaaccat cattaacaac agcatcagca
    17761 tgctggcagc aggtaatagc gtctacttca gtccacaccc gggtgccaaa aacgtttcgt
    17821 taaaactgat taccacaatc gaagacatcg cgtttcgctg tagtggcatt cataacttga
    17881 tcgttaccgt atctgaaccg acctttgctg ccaccgaaca gatgatggcg caccctaaaa
    17941 tcagcgttct ggcgattacc ggcggtccgg ggatcgtcgc catgggaatg aaaagtggca
    18001 agaaagtgat tggtgccgga gcgggtaacc ctccgtgcat cgtcgatgaa acggcggata
    18061 ttgtgaaagc cgccgaggac atcatcaacg gcgcttcatt tgattacaac ctgccgtgta
    18121 ttgctgaaaa aagcctgatt gtcgtggcat cagtggcaga ttatttaatt cagcagatgc
    18181 aaagttttgg cgcacttctt cttaactatg agcaaactga gaaattgcgt gcaatttgcc
    18241 tgccagacgg tagcgccaac aaaaaactgg ttgggaaaag cccatcagcc ttgctggaag
    18301 cggcaggcct ccctttgccc gcaaaagcgc cgcgcctact gattgctgtt gtcgacgccg
    18361 atgatagttg ggttacttgt gagcaactaa tgccaatgct gcccattgtg aaggtcaagg
    18421 attttgacag cgctctaacc ctggcgctga aagtggaaga cggcttgcac cacacggcaa
    18481 ttatgcactc tcagaacgtc tcacgactca accttgccgc gcgcgttatg caaacctcaa
    18541 tatttgtcaa aaacggcccc tcctgggcgg gtattggcgt cggcggcgaa ggctttacga
    18601 cctttaccat tgcaacaccg actggagagg gtacaacgtc agcacggact tttgcccgct
    18661 cacgacgctg cgtgttaacc agcggctttt caattcgcta gagaaacgga aatgaatact
    18721 ttttcactgc agacgcgttt atacagcgga ccaggaagtc tcaaggtact tagccgtttc
    18781 tctcatagac acatctggat tatttgcgat gcctttctgg cacgctcacc acttattgac
    18841 acgttgcatc aggcgctgcc cgacagtaat cgtctcagta tttttagcga aataacgccc
    18901 gccccaacta tccagaccgt ggtgaaaggc attgcgcaaa tgcagacttt acgcccggat
    18961 gtagttatcg gttttggcgg cggttctgcc ctggacgccg caaaagcaat tgtctggttc
    19021 ggtcgccagt gtggcattga aattgaaacc tgcgtagcca ttcctaccac cagcggtatc
    19081 ggttccgaag tgaccagtgc ttgtgtcatc agcgatcctg agaaagggat taaatatccc
    19141 cttttcgata atgcacttta tccggatatc gcgatccttg atccatcttt ggtcgtcagc
    19201 gtgccaccgg cgataacggc caataccggg atggatgttt taacccatgc gcttgaagct
    19261 tatgtttctc ctcgcgccag cgacttcacg gatgcactgg tcgaaaaagc cgtgcaaatt
    19321 gtttttcaat acctgcccac tgcagtgaaa aaaggcgatt gtcttgccac ccgtggcaaa
    19381 atgcataatg catcgacgct tgcaggaatg gcctttagcc aggccggact cggtttaaac
    19441 catgcaattg ctcatcaact cggcggacag tttcatctgc cgcatggact ggcgaatgcg
    19501 ctcctgctca cggcagttat ccgctttaat gctggcgatc cgcgcacagc caaacgctat
    19561 gcccggctgg cgaaaacgtg ccatttatgc ccggacaacg ccaatgacac cgccagcctg
    19621 aatgcactta ttcaacatat tgagcagctt aatacaacct gtacgctgcc gactcttgcc
    19681 aatgcgctga aagaaaaaaa agcagaatgg tcaatacgca taccagatat ggttcaggcg
    19741 gcgctggcgg atgcaacgtt gcgtaccaac ccgcgtgcag ctgatgcctc cgcgattgca
    19801 gaactgctca aggggttgtt atgaacgtcg gattaatctc ctgcgacgac cagaccatcc
    19861 gcgagcgagt acaagcagca ggtgtcgtgg gtgctggcgg cgcaggtttt ccaacccata
    19921 ttaagctaca agcgcgtgct gacactttcc tggtgaatgc ggcggaatgc gaacccatgt
    19981 tgaaagtaga tcaacagtta atggcgcagc aagccagccg cctgcttcgc ggtgttcact
    20041 atgcgatgaa agccaccggg gccagtagcg gaatcatcgc gctgaaagaa aaatatcaac
    20101 gggcaataaa cgcactcaca ccactgttac caccggatat ccgcctgcat attttgcccg
    20161 acgtttaccc cgcaggagac gaagtgctga ccatctggat ggcaactggt cgccgcgttc
    20221 cacccgctgc attacccgtt agcgtcggtg tggtggtcaa taatgttcaa accgtactta
    20281 atattgccag agccgtcgag caacagtatc cagtgacgcg ccgcacactc accgttaatg
    20341 gtgcagtggc gaagccaata acggtaaccg tccccatcgg catgtcttta cgtgaagtcc
    20401 tggcgctggc gggcggcgca acagtggatg ctcctggttt tatcaatggc gggccgatga
    20461 tgggaagttt gatcacatcg ctcgacacac cagtcagcaa aaccactggc ggcttgctgg
    20521 ttttacccaa ctctcattca ctgatccaac gtcgaatgca gaatgaccgc agcgtacttg
    20581 ccgtcgcgaa aactgtttgt gagcaatgcc gcttgtgtac cgatctctgc ccacgtcatt
    20641 taatcggtca cgaactggca ccccatttat tagtacgtgc gcggttaact accaacagct
    20701 cgccacgccg caactgttac tgacagcgct cacctgttca gagtgcaacg tctgcgccag
    20761 tgtcgcctgt ccggtaggaa tttcaccaat gcgcataaac cgcctgctta agcaggaatt
    20821 acgcgcacaa aatctccgct acgaaggtgc cttaaatcct gcagatccca tggcaaatta
    20881 tcgactgatt cccgtaaaac ggctggtaac cagacttggc ttaaccccgt ggtatcagga
    20941 tgcgccattg tctgaacagg tgcctcagcc cgaaaaagtg acgttattgt tacgccagca
    21001 catcggtgcc agcgcaattg cgtgtgtaca aaaaggcgat cgcgtcgttc atgggcaatg
    21061 tgtaggtcaa attccacacg gcacattagg tgcccccatt catgccagta ttgatggcat
    21121 ggtcagcgat gtgacagaaa acgcgattac gcttgtgaga ggttaaccat gtcgaaagcg
    21181 gtaggtattt tagaacttag cagtatcgcg aaagggatgg aaaccggcga cgccatgctg
    21241 aaaagcgcca acgttgagtt actggtcagc aaaacgctat gccccggaaa atttctgctg
    21301 atgctcggtg gcgatgtggg cgcggtgcaa caggccatca acagcggagc gtcccttgct
    21361 ggtgaaatgc tggtcgacag tcttgtcctg ccgaatattc atcccagcct cctttctgca
    21421 atcagcggac ttaaccatgt tgaacagcac cgggctgtcg gcgtagtcga aacctggagt
    21481 gtcgccgcct gcattgacgc tgccgattgt gctgtcaaag ctgcaaatgt cattcttgtt
    21541 cgcgtacata tggcttttgg cattggcggt aaatgctaca tggtggttgc tggcgatatt
    21601 tcagatgtta ataacgcggt ctctgccgcc agcgtgcgcg cgggggaaaa aggtttgttg
    21661 gtctatcggt cggtgattgc ccgtccacat gaagccatgt ggcgtcagat ggtggaggga
    21721 taatgacaac tcagcacaca actgaacgga tgattcagga atatgtccca ggtaagcagg
    21781 tcacgctggc acatgtcatc gccaatccag ggaaggatct gtttaagaaa ttgggtttac
    21841 cagacaccat ttccgctatt ggtattctga caattacccc cagcgaagca tctatcatcg
    21901 catgcgatat cgccacaaaa tcaggcgctg tagagatcgg ttttcttgat cgttttaccg
    21961 gtgccgtagt gctgactggt gatgtttctg cgatcgaata tgcacttagc caggttaccc
    22021 gtacgctggg tgaaatgatg cgctttaccg cctgccccat tacgcggacc tgacgttatg
    22081 caacgtatga tgctcatcgg ccccagccag tgcggaaaaa cgtcactcac gcaatgtctg
    22141 cgtggcgaaa atattcatta tcagaaaacg caggcaatcg tctggtcccc gaccaccatt
    22201 gatacaccag gcgaatacct cgaaaaccgc agcctctaca gtgcactgtt ggtcagtgcc
    22261 tgcgaagcgg acattatcgc gctggtgctt aatgcggacg caccgtggtc acctttttcg
    22321 ccaggcttta cctcgtcaat gaatcgcccg gtcattggag tagtaaccaa agcagattta
    22381 gccaacatgg aacaaatatc tttggtgaaa tgttggttac gggaagctgg agcgcacaac
    22441 gtgttagtaa ccagtgcggt taataaccat ggagttgcag aacttttagc cttgctgcat
    22501 acagaagaag gctgtcgcta atttcgacag gttatcagat gggaatgatg acggtgatac
    22561 acagaaaaga atcaatgagc aatgagggtt gccgggcaac cctcattgaa taaaacagga
    22621 attacttcgc ttcgccgttt ttcatttcgc ccatagcttt cagctttttg gagatatcgc
    22681 ggcgttcttt ggagagctcg gcatttttga tgatgtaatc gtcaacgcga tcttcatagt
    22741 cacctttcat gctggcgatg atgccctgga ttgcttcaac gctcatccca ggcttgatgt
    22801 aatcgctcag gttgtccagc agcaggacgc gcttctggtt gtcacggatc tttttctcaa
    22861 cgtcctgaat ttcacgttgc agtttgttct tacgacggaa cagacgaaca aattccagta
    22921 cgtcctgaaa tgaaggcttg gtagtttcca tttttatacc cctgataatg tgagagtcgg
    22981 attcgtttaa gcaaccgcta ttcgttaggg ccaacattac tcatagctgc cgcagatgac
    23041 aatgctttta tcccttttca ctatcatacc ctttatcctt gctgaatcga agcagcagca
    23101 agatgattct gaagttcagg aattatttgc gcaaggcccg gcaaatcaga ttcgatagca
    23161 actcaagctg atgcgccgtt tccatgttca gccacacata accatagatt ggtgtttcca
    23221 ctaccttcag gtcatggtgg ttattgagca actgacgcag ctcttccaca gcatcgttca
    23281 atttttccgt attggcaaaa accggctgcg gattaccttc gtacagcgca tgaacaaagc
    23341 tcagcagtat ttgctgcatc atgtgctggg tatcacgaag tttttgcgcg ttcaataaca
    23401 cgaaatggct ggggcgcgtg gcccagtacg cattgatttg caactccagc atacaaacca
    23461 gattgcggtt aatggtctgg ataccttcat atatcgattt tggaatacgg gtttctttgc
    23521 tggcgggcgc aatcagccca cgcattttca cggcatcggt caggagtttt tgtagatggc
    23581 tttccagacg tgggcgttcg agtaagttcg gtgagaatgc agattgatag acccgattat
    23641 actcggtcag acttttcgcc agttgaatgc gccagtggat gaacgcccgt tgtggccaga
    23701 taccggtaaa caacattgcc agtaaagagc cgaggatcac atcgccgctt cgccataacg
    23761 ccgtatcaat ttcacctgtc ggggaaccaa caacaattgc cagcgtcacc ccaatcaata
    23821 atccttgata cggtttcttg cccagcgcca gccaaccgca aagaaacatc gccgccgcgc
    23881 accagactaa catcagcggt aacgaaatta actccagttg cagagcgata agacctaaaa
    23941 tcgaacccaa caccgtaccg ccaatacgtt cgaaggcacg agggacaacg ttaccccaga
    24001 acgagattgg ccccataatc accaccatgg tgaccagcgg ccaggtgctt tccgggatag
    24061 taaacaggcg gataatgaga aaagtgagca ggaatgccag cgcgacccga gtaccatgca
    24121 ctatgcggta atggcggtat acccggattt caaacgggct taatgactta tcggcacgca
    24181 cgcagaaact ccgtaatgaa gattaaaagc gctcataact catctgtcgg ccagaaggcg
    24241 cgcctctgaa ttatccgacg gacaaaccgg gaacagtgta atttcccggc aggagcgaaa
    24301 aaattgaaag gcgcatctta ctcttttcgc tttcaaaaaa agagtggtca tcgcgttaac
    24361 acaccgccct gagatgaatt agtgatgttt tggctgcacc gcaacataca tttcgatatc
    24421 ccagtaccca tcttccgcgc cattgttcaa ataaacctcg aagcagggct ttggtaacat
    24481 ttcataagca ctgtcctgca agaggctatt aaagaactga taccagggtt tagcaaaatc
    24541 atcaccgact acacgagcca ccgccaccgc atactgacca cctgaaattt ctgtcagaat
    24601 gacgccctca ctgttttcgg gaagcgtaaa gttattcggt accgtcacaa cggtgtcgca
    24661 gcgtaatttt tcggcgggtg tttcatctgg attgtcgtaa tagacagcaa cccactcctt
    24721 cggcacaata tttttgttat ctacccacat catcaactgc tcaaagcctt tctttaccgt
    24781 ctgttcccac gggccaacga gatggaaacc tgcaacggta cgtttctctt cctgcgtaat
    24841 ctcgtagttc atgacgcctc cattaacact gtttttatat acagcatagt tgcaatatta
    24901 aaaccacaag gaatgagtgt tgattatgcg agcagactcg cactcctgcc agtctgctgc
    24961 aaaagaaagg tcaggcctta tggtggaaat aatcactcag gcgggaaaac atgccgcctt
    25021 ccccgacaga ttccagggta accagcggcc agtgcgccac ctgtttatca cggtcgtaaa
    25081 gttcaatttc ccctacccgc tgatgggcgc taattggcgc ggtgagctct ttaccatcaa
    25141 gggtatattt ggctttgata tgtggaattt cggctttcgg tagcaccatc cagaactctt
    25201 gttccgttcc taggtcgata ttttctttat cgccatacca gatgcgttcc gttccgacct
    25261 ttttcccacg gtgcaaaatt tgcaccgtag taaagttttg ttgcccccaa cgcagtaatt
    25321 ttcttgcctc ttcctcacga ccttttgcac tgtcagcccc cattaccact gcaatgagac
    25381 gacgctgccc atctacagcc gaagcaatga gattaaaccc ggcaccagaa gtatgacccg
    25441 ttttcaggcc gtcaacattc atggttttat cccacaacaa cccgttacgg ttttgctggg
    25501 tgataccgtt ccaggtgaga cttttctcac tgtacatatg ataaaactcg ggctcgccgt
    25561 ggatgatagc gcgagaaaac acagctaaat cataagccga gctatgctgg ccaggtgcat
    25621 ccagaccatg cactgtttca aaatgcgtat ccttgagatg cagcttctcg gcatagttgt
    25681 tcatcatttc aacaaactgc cgttgcccac cggcaatata gtcagccaga gcaacacaag
    25741 cgtcatttcc ggaatccaca attaaaccac ggcttaaatc acgtaccgat acgcgatcgc
    25801 cctctttcaa aaacatcagt gaagaaccga caaacaccgg attatctttc gcccacgcat
    25861 cgcgccccac ggtgacaata tcgtctggcg taatgcgatg actatcgata gcgcgatcca
    25921 cgacataacc cgtcatcagc tttgtcaggc tggcgggatt gcgctgttga tgctcattac
    25981 ccgcggtgag gatctgaccg gtggtgtaat ccatcaatac ccaggacccg gcatgaatct
    26041 ctggaggctg aggtgaaaaa ggaatgtttt ccgccgcaaa accagacgat aagttaaaaa
    26101 cgaacaaaga agcagcaata ataagacggc gtttcaacag caaaccctca ggagtttcaa
    26161 atagctgttc tttttacgga aatacttatg aactggctgg aataaagtgc aagaaaatgt
    26221 gactaccctc tcatttttat ctgacatgat ctgttgccac tcgctgccaa attgtggcgc
    26281 taaagctgat tagcacggtg atatttgata ctctggcaga cagcagaaat aacggattta
    26341 acctaatgat gaatgacggt aagcaacaat ctaccttttt gtttcacgat tacgaaacct
    26401 ttggcacgca ccccgcgtta gatcgccctg cacagttcgc agccattcgc accgataacg
    26461 aattcaatgt catcggcgaa cccgaagtct tttactgcaa gcccgctgat gactatttac
    26521 cccagccagg agccgtatta attaccggta ttaccccgca ggaagcacgg gcgaaaggag
    26581 aaaacgaagc cgcgtttgcc gcccgtattc actcgctttt taccgtaccg aagacctgta
    26641 ttctgggcta caacaatgtg cgtttcgacg acgaagtcac acgcaacgtt ttttatcgta
    26701 atttctacga tccttacgcc tggagctggc agcatgataa ctcgcgctgg gatttgctgg
    26761 atgttatgcg tgcctgttat gccctgcgcc cggaaggaat aaactggcct gaaaatgatg
    26821 acggtctacc gagctttcgc cttgagcatt taaccaaagc gaatggaatt gaacatagca
    26881 acgcccacga tgcgatggct gatgtgtacg ccactattgc gatggcgaaa ctggtaaaaa
    26941 cgcgtcagcc acgtctgttt gattatctct ttacccatcg taataaacac aaactgatgg
    27001 cgttgattga tgttccgcag atgaaacccc tggttcacgt ttccggaatg tttggggcat
    27061 ggcgcggcaa taccagctgg gtggcaccgc tggcgtggca tccagaaaat cgcaatgccg
    27121 taattatggt ggatttggca ggagacattt cgccattact ggaactggat agcgacacat
    27181 tgcgcgagcg tttatatact gcaaaagccg atcttggcga taacgccgcc gttccggtta
    27241 agctggtgca tatcaataaa tgtccggtgc tggcccaggc gaatacgcta cgcccggaag
    27301 atgccgaccg actgggaatt aatcgtcagc attgcctcga taacctgaaa attctgcgtg
    27361 aaaatccgca agtgcgcgaa aaagtggtgg cgatattcgc ggaagccgaa ccgtttacgc
    27421 cttcagataa cgtggatgca cagctttata acggcttttt cagtgacgca gatcgtgcag
    27481 caatgaaaat tgtgctggaa accgagccgc gtaatttacc ggcactggat atcacttttg
    27541 tcgataaacg gattgaaaag ctgttgttca attatcgggc acgcaacttc ccggggacgc
    27601 tggattatgc cgagcagcaa cgctggctgg agcaccgccg ccaggtcttc acgcccgagt
    27661 ttttacaagg ttatgcagat gaattgcaga tgctggcaca acaatatgcc gataacaaag
    27721 agaaagtggc gctgttaaaa gcgctttggc agtacgcgga agagatcgtc taatttgaag
    27781 cctccgccgc tggtacggcg gaggtcaatg ttgagtcagg ctttttgaac ggtgatactc
    27841 cacgctgcat cgccaatctg ctgataatcg gtgatggcat gcccttcttc tgccgcccac
    27901 tgtgggattg cttcagttgc ctgggtgcaa tcaaactcaa ttaccaactc atcgccgctg
    27961 gccatttctg ccagtgctgc ttttgcttct atgagtggaa acggacagac ctgcgtcacc
    28021 acatccagct ttttaatcgc catctttaca ccttacttaa tttgccgcag cagttgccag
    28081 tcgcgcctta cgctgcggtc gaacatacac aagccaggac gcagtccaga ctccgagaat
    28141 cataaataca aggccaatcc agccctgcca ggtcatcatt gcagtcataa ccagtccatt
    28201 accgataaag caaccacctg caatactggc accgaagccc atcagtacac cacctaaccc
    28261 gctacgtaat gttgtttgtg catcagctgc gcgaacgcgg aactcacggc tcgctttggc
    28321 tgcaataaat gaccccacga agatccctaa caccaggaaa acgccccagt taatgtattt
    28381 cacgtcaccc gcgaccagaa attgcagaat attggccgtt ggggaagtga ttcctaaccc
    28441 aaacatgcgt ccggttgctt cactcagggg ccaggctaaa agcgcaatca aaccgatgag
    28501 tacagcagtg acaaagggat gccagcgttt ttcaaacaga atatgagcga tcccagttcg
    28561 gcgcggcggt aaggtcgcga ctttgagttt tggcttcttc aactctttca tcaccaccca
    28621 gagcgttatc accagcaaaa cggcaaccaa cggccaaaca gacaaattaa aagtctcagc
    28681 aatagagtta tgttcagtac tgtagtgctg caaggtttga tttaaaccac tggcatgtgg
    28741 agaacgcatc accgcactca tcaccatata agtgaaaagc gcgatccaac tgccgatcaa
    28801 tccctcaccc gcgcgatacc aggtcccggt ggcacatcca cccgccagaa caattcccag
    28861 cccgaagata tacccaccta taacagtacc aagccacggg aacgcctcgg cttcgtaagt
    28921 cagtaggccc gcctgaatta acgcaaaaac ccccacgctt tgtaccgaaa ttgcaatcag
    28981 caaggcgtaa aacatgcgat tatttttcgc gatatacata tcgcgaaaac cacctgtcag
    29041 gcaaaaacgc ccacgctgca tgacaaatcc cagcagagca ccacaaatta gcccgcttaa
    29101 tatcattgaa aacataaatt aattaaccag atgaatgtta atgaggaaat tattcatgac
    29161 tggtg
//
LOCUS       DAZPAC010000034        28678 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZPAC010000034 DAZPAC010000000
VERSION     DAZPAC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.034"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..1046)
                     /locus_tag="ACHUPM_001216"
     CDS             complement(<1..1046)
                     /locus_tag="ACHUPM_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0869054.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSILPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..2094
                     /gene="pinE"
                     /locus_tag="ACHUPM_001217"
                     /pseudo
     CDS             1537..2094
                     /gene="pinE"
                     /locus_tag="ACHUPM_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2433..3281)
                     /locus_tag="ACHUPM_001218"
     CDS             complement(2433..3281)
                     /locus_tag="ACHUPM_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR0869055.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3490..4125
                     /gene="yfhb"
                     /locus_tag="ACHUPM_001219"
     CDS             3490..4125
                     /gene="yfhb"
                     /locus_tag="ACHUPM_001219"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR0869056.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4183..4686
                     /gene="tadA"
                     /locus_tag="ACHUPM_001220"
     CDS             4183..4686
                     /gene="tadA"
                     /locus_tag="ACHUPM_001220"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR0869057.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQPSTD"
     gene            complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="ACHUPM_001221"
     CDS             complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="ACHUPM_001221"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR0869058.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6497..10384
                     /gene="purL"
                     /locus_tag="ACHUPM_001222"
                     /gene_synonym="purI"
     CDS             6497..10384
                     /gene="purL"
                     /locus_tag="ACHUPM_001222"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR0869059.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10942..12369
                     /gene="qseE"
                     /locus_tag="ACHUPM_001223"
     CDS             10942..12369
                     /gene="qseE"
                     /locus_tag="ACHUPM_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR0869060.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12534..13247
                     /gene="qseG"
                     /locus_tag="ACHUPM_001224"
     CDS             12534..13247
                     /gene="qseG"
                     /locus_tag="ACHUPM_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR0869061.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13237..14571
                     /gene="glrR"
                     /locus_tag="ACHUPM_001225"
     CDS             13237..14571
                     /gene="glrR"
                     /locus_tag="ACHUPM_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR0869062.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14632..14970
                     /gene="glnB"
                     /locus_tag="ACHUPM_001226"
     CDS             14632..14970
                     /gene="glnB"
                     /locus_tag="ACHUPM_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR0869063.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="ACHUPM_001227"
     CDS             complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="ACHUPM_001227"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR0869064.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16533..17786
                     /gene="glyA"
                     /locus_tag="ACHUPM_001228"
     CDS             16533..17786
                     /gene="glyA"
                     /locus_tag="ACHUPM_001228"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR0869065.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(17984..19177)
                     /locus_tag="ACHUPM_001229"
                     /pseudo
     CDS             complement(17984..19177)
                     /locus_tag="ACHUPM_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19295..22572
                     /locus_tag="ACHUPM_001230"
                     /pseudo
     CDS             19295..22572
                     /locus_tag="ACHUPM_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22669..23652
                     /locus_tag="ACHUPM_001231"
     CDS             22669..23652
                     /locus_tag="ACHUPM_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFR0869066.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23675..25186
                     /locus_tag="ACHUPM_001232"
     CDS             23675..25186
                     /locus_tag="ACHUPM_001232"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR0869067.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25211..26209
                     /locus_tag="ACHUPM_001233"
     CDS             25211..26209
                     /locus_tag="ACHUPM_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0869068.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26242..27336
                     /locus_tag="ACHUPM_001234"
     CDS             26242..27336
                     /locus_tag="ACHUPM_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR0869069.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27348..28220
                     /locus_tag="ACHUPM_001235"
     CDS             27348..28220
                     /locus_tag="ACHUPM_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR0869070.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28268..>28678)
                     /locus_tag="ACHUPM_001236"
     CDS             complement(28268..>28678)
                     /locus_tag="ACHUPM_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0869071.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6818 a         7093 c         7790 g         6977 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg caagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtatggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtga ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctactttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt tgaactgtgc aggatgcgag
     1621 ctgatttttg aagacaagat aagtggcaca aagtccgaaa ggccggggct gaaaaaactg
     1681 ctcaggacat tatcggcagg cgacacactg gttgtctgga agctggatcg gctggggcgc
     1741 agtatgcggc atcttgtcgt gctggtggag gagttgcgcg aacgaggcat caactttcgt
     1801 agtctgactg attcaattga taccagcaca ccaatgggac gctttttctt ccatgtgatg
     1861 ggtgccctgg ctgaaatgga acgtgaactg attgttgaac gaacaaaagc aggactggaa
     1921 gctgctcgcg catagggacg aattggcggt cgccgtccca aactttcacc agaataatgg
     1981 gcgcaggccg ggcgactaat tgcatcagga gtttctcgcc agaaggtggc gatcatctat
     2041 gatgttggcg aatcgacttt tttgtataag aggtttccag ccagggttat ataaaaccgc
     2101 agacacggcg tatacaagaa cgtgctacgg ttgactgaaa agttaatgtg gtatgtcaca
     2161 ggccttttgc gccgtaaagc cccgtccttt agggcgcgga ggacgttaaa gaaagtggtg
     2221 ttctttgtgt tgctgaactt atgcaaaatt tttttgcacc ataaatccaa caacacattc
     2281 aataacacat tgctgtatca ctatgaaaat aaatgtgttt ttatttgatt tcacacatat
     2341 cacaattgat gcatttttta gtgattaaca acgccataag gaaactctca aaacaacaca
     2401 aaccgggcgg gcattgtacg cgcattcttt actcagacca gttttttcac cagcgcttca
     2461 ctatgacgaa tacgttctgg tgccagttcc agatcctgct gaatcagcgc aatgaacagc
     2521 aaatccgtta acattccctg agcgtgacaa gcagagattg aagcactgtt tgtcgcttgt
     2581 tcttcggcaa tggtatacag gcaatgagaa gcacgctgtt gcagggcatt cggagtaaag
     2641 ccggtaatcg ccagcacttt tccgcccact cgcagcatct catctgccgc caggtttaac
     2701 tcgcgtcgta caccggtgta ggaaatggct aataacagat cgtcagggga cgacgcctgt
     2761 actgttgcga gtagcgcatg catatcgcgc actgcggcag cattgaagcc aatcttcatc
     2821 agcttccagg caaagttttg cgccaccaga cccgaagcgc caataccggt cagaattatc
     2881 cgccgcgcag agcgcaacat tgttacgcat tcatgcagtt tctcttcact attaacgttt
     2941 agcgttgcgt acatagcggc agtattttct ttaatcagtt tttcgccgac cagccgtaac
     3001 ggatcatcac cgcggatttg gttatgaatg ggcacggagg gcgattccgg ctggcttgcc
     3061 agcgcttcac tcaacgccaa cttaagcgcc ggaaaacctt tatagccgag tttttgcgcg
     3121 aacttcacga cgctggactg actgactccg gcttcgttgg ccagttgctg agagcttaaa
     3181 tggcgcgccg tatcaggttg tagcagcaga taatccgcca gttttttatc actctgggca
     3241 agcccctggt aacgctgacg gatacgaagt aagccgttca tatctttcct cgcgatgaaa
     3301 tagtggcgac aacaggcaat atgagacaca cgcgagtgaa ttttatattc cattgccgct
     3361 aattgccatg aattaaaaat tccagttagc catgatgcgg tcgattgttg ccggaaaagt
     3421 gatgatctat ctgttaggct atgtagtagc gaaaattaat caggatattt cagtccagag
     3481 gagtatggtt tggcaactca cgagcgtcgt gtggtgtttt ttgacttaga tggaacattg
     3541 catcagcagg atatgttcgg cagttttctg cgctatttac tgcgtcgcca accgctgaat
     3601 gcgttacttg tcctgccgtt gttaccgatt atagccattg cgttattgat aaaaggtcgt
     3661 gcggcacgct ggccgatgag tctgcttctg tgggggtgca cttttggtca cagcgaagca
     3721 cgtttacaga cgttgcaggc cgatttcgtg cgctggtttc gcgacaatgt taccgccttt
     3781 ccgctggttc aggagcgatt aaccacctac ctgttaagtt ccgatgctga tatctggttg
     3841 attaccggct ctccgcagcc gctggttgaa gcggtttatt tcgatacgcc ctggctgccg
     3901 cgggttaatc ttatcgccag ccaaattcag cgtggctatg gtggttgggt attgacgatg
     3961 cgttgtctgg gacatgaaaa ggtcgcacaa ctggagcgca aaatcggcac tccgctgcgg
     4021 ctgtacagtg gctatagcga cagtaatcag gacaatccgc tgctttattt ctgtcagcat
     4081 cgttggcgag taaccccgcg cggtgaactc cagcaactgg aatagagtaa agcatagcgt
     4141 ccgtgtataa tgcgccgcgc ttttataacc ggagttttct ttttgtctga agtcgaattt
     4201 agccacgaat actggatgcg tcacgcgctg acgctggcga aacgtgcctg ggatgagcgg
     4261 gaagtgccgg tcggcgcggt attagtgcat aacaatcggg taatcggcga aggctggaac
     4321 cgcccgattg gtcgccatga tcccaccgca catgcagaaa tcatggccct gcggcagggt
     4381 ggtctggtga tgcaaaatta tcgtctgatc gacgccacgt tgtatgtcac gcttgaacca
     4441 tgtgtaatgt gtgccggagc gatgatccac agtcgcattg gtcgcgtggt ctttggtgcg
     4501 cgtgacgcga aaactggcgc tgcgggatct ttaatggatg tgctgcatca tccgggtatg
     4561 aatcaccgag tggaaattac ggaaggaata ctggcggatg agtgcgcggc gttgctcagt
     4621 gacttctttc gcatgcgccg ccaggaaatt aaagcgcaga aaaaagcgca accctcgacg
     4681 gattaatttt gtttctcttc actccctttc ctggaaaaca gcagagaggg agaatgggtc
     4741 aaatagtttg atgacgactg ggaaagaaac gagagaaaag gaaatttctc tttgcccaac
     4801 tccgtaggcg ataccgccgg ataatcctgc gccagttgca tcgccgcttc tgtagcctgc
     4861 ttctctttct cttgcagata acccaccagg ctaatctgat acttacgaat attttcgaca
     4921 taagcgtagg cttcatgtcc acgagcgtag ccgtaagtca gcttgctgta atagggtttc
     4981 tggctaagta aaggcagacg ctgttttacg tcagcccaac tgtcaggatt ccctttggtt
     5041 tttgccgtca gggcgcgggc atccagcata tgcgcatagc ccatattgta cgcagcgagg
     5101 gcaaaccaga tccgctcgtt ctccggcaca ctttccggca ctttactcat catatcctgc
     5161 aaataacgca cgccaccgct gatgctctgt tcggcatcgg tacgatccgt aatgccgagg
     5221 ctttgcgcgg tatttttggt taacatcatc atgccgcgca cacccgtcgg tgaagtggcc
     5281 tgtgcatccc agtgcgattc ctgataagca atagcggcca gcaaacgcca gtcaatttct
     5341 tcggcgtatt tctcaaacag gggctttaac tgcggcagta ccgcatcgac ggcgcgtaaa
     5401 aatgtgcgcg tatcgacgta atcaaaatca tcgccatgcc ccaggtattt ctcttcaatg
     5461 cgtgccagcg taccgtcttc attcatttcg ttgaagaagt cgagcagggc ggcggaaagg
     5521 gtattatcgc catctaacgg gctaaaccaa gtcaccggtt gttcatcggt gatatcgagc
     5581 gctacggcga gctccgggtg aacgcgctga aacaggctga tggcgacaga atcagcaatg
     5641 gtgtaatcga gttttccttc gatgacatct tccattaatt ccgcagagcc ttttttgtcg
     5701 tctaccttcc agcttaattc cgggaatttt gtgtctttca gggtttggag atcgttaacc
     5761 accacgtgac ccggtgcaac ggtgagttgc tccgccgtca ggttgcccag tgtgcgcggg
     5821 cgatactgac tcactttata aaccagttgt tgtgacacgg aataataggt agggccaggc
     5881 tgataatttt ttacccgctc actgttatag accagtcctg ccgccagcag gtcggcgtta
     5941 ccattatcga ggtcgtcaaa cagctggctg atattctgcc gcacggtcac tttcagtttt
     6001 acaccgaggt aatcggcaaa ctgtttcgcc agttcgtaat ccaggccaaa aggtttcccg
     6061 ttgatttcgt tataagtcag gggagtatga atggtgctca cacgcaactc tccccgcgct
     6121 tgaatggcgg cgatacggtt gtcggcttta ccaaaccagg gaatggatgg ccagagagcg
     6181 accgcgagca gcagtgccag aatgccgatg aacagataat taatctttaa ttttttcaat
     6241 tagttaattc tctgtgtcgt gcgcgtccca gcttgaaaaa acgtaataat agtgaaaggt
     6301 ttactcataa atgagcggca ttttgcgtaa acctgcgcca gatggcaact tattacagcc
     6361 attggcggca cgcgttgcta attcacgatg gtgattttat ttccacgcaa acggtttcgt
     6421 cagcgcatca gattctttat aatgacgccc gtttcccccc cttgggtaca ccgaaagctt
     6481 agaagacgag agacttatga tggaaattct gcgtggttcg cctgcactgt cggcattccg
     6541 aatcaacaaa ctgctggcac gttttcaggc tgccaggctc ccggttcaca atatttacgc
     6601 cgagtatgtc cattttgctg acctcaatgc gccgttaaac gatgatgagc acgcacaact
     6661 tgaacgcctg ctgaaatatg gcccggcact cgccagccac gccccgcaag gcaaactcct
     6721 gctggtgacc ccgcgtcctg gcaccatctc tccctggtct tcgaaagcga ccgatattgc
     6781 ccataactgc gggctacaac aggtaaaccg ccttgagcgc ggcgttgctt actatataga
     6841 agccggtacg ctgaccaatg aacaatggca gcaggttacc gctgaactgc acgaccgcat
     6901 gatggaaacg gtcttttttg ctttagatga tgcagagcag ttgtttgccc accatcaacc
     6961 gactccggtt accagcgttg atttgctggg gcagggccgt caggcgctga tcgacgctaa
     7021 cctgcgtctt ggcctggctc tggcggaaga tgaaattgac tatctgcagg atgctttcac
     7081 aaagcttggt cgtaacccga acgacatcga gctgtatatg ttcgctcagg cgaactctga
     7141 gcactgccgc cacaaaattt ttaatgccga ctggattatc gacggtgaac agcagccgaa
     7201 atcgctgttc aagatgatca aaaatacttt cgaaaccacg ccagatcacg ttctctctgc
     7261 ttataaagat aacgccgccg taatggaagg ttctgaagtg ggccgctact ttgctgacca
     7321 cgaaacgggc cgctacgatt tccatcagga accggcgcat attctgatga aagtcgaaac
     7381 tcacaaccac ccgacggcga tttctccgtg gccgggcgcg gcgacgggtt ccggcggcga
     7441 aattcgtgat gaaggcgcca ccggacgcgg tgcgaagccg aaggctggtc tggttggttt
     7501 ctccgtttcc aacctgcgaa ttcctggctt cgaacagccg tgggaagaag atttcggtaa
     7561 gcctgagcgc attgtcaccg cgctggacat catgaccgaa ggcccgctgg gcggcgcggc
     7621 gtttaacaac gaatttggtc gtccggcact gaatggctac ttccgtactt atgaagaaaa
     7681 agtgaacagc cacaacggcg aagagctgcg cggttatcac aaaccgatca tgctggcggg
     7741 cgggatcggc aacattcgcg ccgatcacgt acaaaaaggc gagatcaacg tcggtgcgaa
     7801 gctggtcgtt ctcggcggcc cggcaatgaa catcggtctt ggcggcggag cggcgtcttc
     7861 catggcgtca ggtcagtctg atgccgacct cgactttgct tccgtacagc gcgacaaccc
     7921 ggagatggag cgtcgctgcc aggaagtgat cgaccgttgc tggcagcttg gtgatgccaa
     7981 cccaatcctg tttatccacg acgtcggcgc tggcggtctt tctaacgcga tgccggaact
     8041 ggtgagcgac ggcgggcgcg gcggtaaatt tgaactgcgc gatattctaa gcgacgaacc
     8101 gggcatgagc ccgctggaaa tctggtgtaa cgaatcccag gaacgctacg tgctggcggt
     8161 tgctgccgat caattaccgc tgtttgacga actgtgtaag cgtgagcgcg caccctacgc
     8221 ggtgattggt gaagcgaccg aagaactgca tctttctctg cacgatcgtc attttgataa
     8281 tcagccgatc gatctgccgc tggacgtcct gcttggtaaa acgccgaaga tgacccgcga
     8341 tgtacaaacg ctgaaagcga agggcgatgc gctggtccgt gaagggatca ccattgcaga
     8401 cgcggtgaaa cgcgtactgc atctgccgac cgtggcagag aaaaccttcc tggtgaccat
     8461 cggcgaccgc agcgtaaccg gcatggtagc gcgcgatcag atggtggggc cgtggcaggt
     8521 gccggtcgct aactgcgcgg tcactaccgc cagcctcgac agctactacg gtgaagcgat
     8581 ggcgattggc gagcgtgcgc cggttgcact gctggatttc gccgcctctg cccgtctggc
     8641 ggtcggtgaa gcgttaacca atattgccgc aacacagatt ggcgatatca aacgcattaa
     8701 actttccgcc aactggatgg cggcggcagg ccaccctggt gaagatgcgg gcctgtatga
     8761 agccgttaaa gccgtgggcg aagagctttg tccggcgctg ggcctgacga tcccggtggg
     8821 taaagactct atgtcgatga aaacccgctg gcaggaaggt aacgaagagc gcgaaatgac
     8881 gtcgccgctg tcgctggtga tttctgcatt tgcccgcgtg gaagatgtgc gtcacaccat
     8941 cacgccacag ctttctaccg aagataacac actgctgctg attgatttgg gcaaaggcaa
     9001 taacgcgctg ggcgcaacgg cgctggcgca ggtttatcgt cagcttggcg acaaaccggc
     9061 agatgtacgc gacgtcgcgc aactgaaagg cttctatgac gcgattcagg cgctggttgc
     9121 acagcgtaag ctgctcgcat accacgaccg ctctgatggc ggcctgctgg taacgctggc
     9181 ggaaatggcc tttgctggtc attgcggcat taacgcggat attgcctctc tgggcgacga
     9241 tcgcctggcg gcgttgttta acgaagaact gggtgcggtg attcaggttc gtgccgctga
     9301 ccgtgaagcg gtcgagtccg tactggcaca gcatgggctt gctgattgtg tccattatgt
     9361 agggcaggcg gtttccggtg accgttttgt gattaccgcc aacgggcaga ctgtattcag
     9421 cgaaagccgc accacgttgc gtgtctggtg ggcagaaact acctggcaga tgcagcgcct
     9481 gcgtgacaac ccggagtgtg ctgaccagga gcaccaggcg aaatcaaacg acgccgatcc
     9541 gggcctgaat gtgaaactgt cgttcgatat caacgaagat gtggcagcac cgtatattgc
     9601 cactggcgca cgtccgaaag ttgctgtgct gcgtgagcag ggcgtgaact cgcatgttga
     9661 aatggcggca gctttccacc gtgcaggctt tgatgctatc gacgtgcata tgagtgacct
     9721 gctgaccgga cgcacgggcc tggaagattt ccacgccctg gtcgcgtgcg gtggtttctc
     9781 ctacggtgat gtgctgggtg ccggtgaagg ttgggcgaag tcaatcctgt tcaatgaccg
     9841 tgtacgcgat gagtttgcaa ccttcttcca ccgtccgcaa acgctggcgc tgggggtatg
     9901 taacggttgc cagatgatgt ctaacctgcg tgaactgatc ccggggagcg agttgtggcc
     9961 acgttttgtg cgtaacacct ccgatcgctt tgaagcgcgt ttcagcctgg ttgaagtaac
    10021 ccaaagcccg tctctgctgt tgcaggggat ggtgggctcg caaatgccga ttgctgtctc
    10081 tcatggtgaa gggcgcgtgg aagtgcgtga tgcggcgcat ctggcggcac tggaaagcaa
    10141 agggctggtg gcactgcgct atgtcgataa cttcggcaaa gtcactgaaa cctacccggc
    10201 taacccgaac ggttcaccga acggtattac ggcggttacg actgaaagtg gtcgagtcac
    10261 cattatgatg ccgcacccgg aacgtgtttt ccgtactgtc agcaactcct ggcatccgga
    10321 aaactggggc gaggatggcc catggatgcg cattttccgc aatgcgcgta agcagttggg
    10381 gtaagtcatc agttcattgg ttttgcagcc cggatgcggc attaagcacc aaccactatc
    10441 ataagctccc aactccggga gcttttttgt gtctgtaaat cacgacaatg ggtggtttac
    10501 cgtgtcgctt tctggcgaca cttaaatgat tgattttatt gcaatctaat aagtttatct
    10561 taagttgtct ctttttagcg acacagtggc tgatttacat tcaaatagcg aagaagccat
    10621 aaatccctca aagcaacgta taatcagtac gttaccaaac tattttcttt attggcacag
    10681 ttactgcata atagtaagca gtggctcatt caccgactta tgtcagcccc ttcgggacgt
    10741 gctacataaa attcgaatga cgcacaacaa ggtgcctgcc gtccaacttc tgatatcagc
    10801 gtagctatat caaccatcgg gcgaaacgtc gagttaggca ccgccttatt ccataacaaa
    10861 gccgggtaat tcccggcttt gttgtatctg aacttcccct cggttagcat caggctattc
    10921 gcgtctgacg agagtaacac cttgaaacgc tggcccgttt ttccccgctc attacgacaa
    10981 ctggtaatgc tggcattttt gctaattcta ctacccctgt tggtgctggc atggcaagcc
    11041 tggcaaagcc tgaatgcgct tagcgatcag gcggcgctgg ttaaccgcac tacgcttatt
    11101 gatgcccggc gtagtgaagc gatgaccaac gcggcgctgg agatggagcg tagctaccgt
    11161 cagtattgcg tgctggacga ccctacgctt gcgaaggttt atcaaagcca gcgcaagcgt
    11221 tacagcgaaa tgctcgatgc ccacgcaggc gtactgccag acgataaact ttaccaggca
    11281 ttgcgtcagg atttgaacaa tctggcccaa cttcagtgta acaacagtgg tcccgatgct
    11341 gccgccgccg cgcgtcttga agcctttgcc agtgccaata ccgaaatggt gcaggccacg
    11401 cgcacagtgg tgttctctcg tgggcagcaa cttcagcgtg aaatcgccga acgtgggcaa
    11461 tattttggtt ggcaatcgct ggtgctattt ctggtgagtc tggtgatggt gctacttttc
    11521 acgcggatga ttatcgggcc ggtgaaaaat atcgagcgca tgatcaaccg tctgggggaa
    11581 gggcgttctc tgggcaatag cgtttcgttc agtggaccga gcgagttacg ctcggttggg
    11641 caacgtattc tttggttaag tgagcgcctg tcatggctgg aatcccaacg ccatcaattt
    11701 ttaagacatt tatctcatga attaaaaacg ccactggcga gtatgcgcga gggcactgaa
    11761 ttactggctg accaggttgt cgggccgctt acgccagagc aaaaagaggt ggtgagcatt
    11821 cttgatagca gcagccgcaa tttgcaaaaa ctgatcgaac aactgcttga ttacaaccgt
    11881 aaacaggcgg acagtgcggt ggaactggag aatgttgaat tagcaccgct ggtggagaca
    11941 gtggtttctg ctcatagcct gcccgcacgg gctaaaatga tgcataccga cgtcgatctc
    12001 aaagcaacag cttgcctggc ggagccaatg ctgctgatga gcgtactgga taatctttac
    12061 tccaatgcgg tgcactacgg ggctgaatcc ggtaacattt gccttcgcag cagttcacat
    12121 ggtgcgcggg tttatattga tgtcatcaat acaggcacgc ccattccgca agaggaacgc
    12181 gccatgatct tcgaaccctt ttttcaggga agccaccagc gaaaaggggc ggtgaagggc
    12241 agcggtctgg gattaagcat tgccagggat tgtattcgcc gtatgcaagg ggaactgtat
    12301 ctggtcgacg agagcgggca agacgtttgt ttccgcattg aattaccgtc gtcgaaaaac
    12361 acgaaataat gctaacagcc tatcctatca ggctatttta tttgtcagtt cggccccggg
    12421 cagtgctcga aatcttcacg tacgatatgt acgctccggt ttctccgcgc tgtccatgtc
    12481 cagactgtct gcaacaatta cgcccactgg gataggcttt aagtctggtg aatatgcgac
    12541 acatttttca acgattattg ccccgacggc tgtggctggc agggctgcca tgtctggcat
    12601 tgctgggttg cgtgcagaat cacaataagc cagccattga tacgccagca gaagaaaaaa
    12661 ttccggttta tcaactggct gactatcttt ctactgaatg tagcgacatc tgggcgctgc
    12721 aaggtaaatc aaccgaaacc aatccgcttt actggctgcg ggcgatggat tgtgctgatc
    12781 gtttaatgcc tgcgcagtcg cgtcagcaag ctcgccaata tgacgacggc agctggcaaa
    12841 atacctttaa acaagggatt ttgctcgccg acgccaaaat tacgccgtat gagcgccgcc
    12901 agctggtcgc gcgtattgaa gcgttaagta ccgagatccc ggcacaggtt cgcccactgt
    12961 atcaactctg gcgcgacggt caggcgctgc aactgcaact ggcggaagag cgccaacgtt
    13021 acagtaaact ccagcagtcc agcgacagcg agctggatac attgcgccag caacatcacg
    13081 ttctacagca gcaactggaa ctcaccaccc gcaagctgga aaacctgacc gatattgaac
    13141 gccagctctc gacccgcaaa ccgactggaa atttctcgcc ggatacgccg cacgagagtg
    13201 aaaagcccgc gccatccacc catgaggtca ctcctgatga gccataaacc tgcgcattta
    13261 ttattggtcg atgacgatcc gggattgctg aaactgcttg gcctgcgcct gaccagcgaa
    13321 ggctacagtg tggtcacggc ggaaagtggc gctgaaggat tacgggtact gaatcgcgaa
    13381 aaagtagatt tagttatcag cgacctgcgg atggatgaaa tggacggtat gcagctattt
    13441 gctgaaatcc agaaagtgca gccgggaatg ccagtaatta ttcttactgc gcatggttct
    13501 atccccgatg ccgttgctgc aacacagcag ggcgttttta gtttcctcac caagcctgtc
    13561 gataaagatg cgctatatca ggcaattgac gatgcgctgg agcaatccgc gccagccacc
    13621 gatgaacgct ggcgcgaggc aattgtcacc cgcagcccgc tgatgctgcg tttgctggaa
    13681 caggcgcggc tggtggcgca atcagacgtc agcgttttga ttaacggtca gagcggcacc
    13741 gggaaagaga ttttcgccca ggctatccac aacgccagcc cgcgcaacag caaaccattt
    13801 attgctatta actgtggcgc attgcccgag caattgctgg agtcggagct gtttggtcat
    13861 gcgcgtggcg cgtttactgg cgctgtcagc aatcgcgaag gtttattcca ggcggcggaa
    13921 ggcggaacgc tatttctcga tgaaattggc gatatgcctg cgccgttaca ggtcaaactg
    13981 ctgcgcgtgt tgcaggagcg taaagtgcgc ccgctgggca gtaaccgcga tattgatatc
    14041 aatgtgcgga ttatttctgc cactcaccgt gatctgccaa aagcgatggc gcgcggggaa
    14101 ttccgcgaag atctctatta ccgcctcaac gttgtcagcc tgaaaattcc ggcactggcg
    14161 gagcgcacag aagacattcc gctactggca aatcacctgt tgcgccaggc ggcagagcga
    14221 cataaaccgt ttgtccgcgc gttctctacc gatgcgatga aacgcctgat gaccgcgagc
    14281 tggccgggta atgtgcgcca gttggtcaac gtgattgaac agtgcgtggc gctgacctca
    14341 tctccggtga ttagtgatgc gctggtggag caggcgctgg agggtgaaaa tacggcgctg
    14401 ccaacctttg ttgaggcacg taatcagttt gaactcaact atttgcgtaa gctgctgcaa
    14461 atcaccaaag gcaacgtcac ccacgcggcg agaatggcgg ggcgcaaccg gacagaattt
    14521 tataaactgc tttcccgaca cgagctggat gcaaacgatt tcaaggaatg aattggcgtt
    14581 atgtgttacg tttagcagat caaaagacag gcgacctttt caaggaatag catgaaaaag
    14641 attgatgcga ttataaaacc cttcaagctg gacgatgtcc gcgaagcact ggccgaagtc
    14701 ggtattaccg gcatgacggt gaccgaagtg aaaggctttg gtcgccagaa aggccatacc
    14761 gagctgtacc gcggcgcgga gtatatggtg gattttctgc cgaaagtgaa aattgagatt
    14821 gtcgtaccgg acgacattgt cgatacctgt gtcgatacca ttattcgcac ggcgcaaacc
    14881 ggcaaaatcg gtgacggtaa aatcttcgtc tttgacgtgg cacgggtcat tcgcatccgt
    14941 accggtgaag aggacgacgc ggcaatttaa accgcgtcaa gggttgccgg atgtttccat
    15001 ccggcaacat caaattacag caccttatgc gggccaaagc attcgtaatg aatgttttcc
    15061 tgcttcacgc ccagatccac taactgtttc gcggtaaact gcatgaagcc aaccgggccg
    15121 catagataga actgcattgt cggatcgctg aacgcacctt ccagtttgct caaatccatc
    15181 agaccttcgc tatcaaactg acctttagcg cgatcggctt cgctcggctg acgatgccag
    15241 gtgtgcgcgg taaagcgcgg cagtgactgc cccagttcct taacttcatc ggcaaaggcg
    15301 tgaacatcgc cattttctgc cgcatggaac cagttcactt gtgctgtgtg gcctgctttt
    15361 gccagcgtgt cgagcattgc cagcattggc gtttgaccaa caccggcaga gattaacgtc
    15421 actggtgtgt catctgcgac agccataaag aaatcacctg ccggagcgac cagtttcacg
    15481 acatcgccaa cattggcgtg attgtgcaac cagttggata cctgcccacc ctcttcgcgt
    15541 ttcaccgcaa tacgatagcc tttgccatcc ggtttgcgag tcaaagagta ctgacgaatt
    15601 tcctgatgcg ggaaaccttc cggcttcagc cagacgccga gatattgccc cggacggtat
    15661 tctgccactg cgccaccgtc gaccggctcc agttcgaagc tggtgataag cgcgctgcgc
    15721 ggtgttttag ccacaatgcg gaaatcgcga gtaccttccc aaccaccggc tttgctggcg
    15781 ttttcgttat agatttccgc ctcgcgattg ataaatacat tagccagtac accataggct
    15841 ttaccccacg cgtccagcac ttcctgcccc gggctgaaca tttcgtccag cgttgccaac
    15901 aggtgttcac cgacgatgtt gtactgttcc ggtttgatct ggaagctggt gtgcttctgc
    15961 gcgatttttt ctaccgctgg cagcagcgca ggcaggtttt caatattact ggcgtaggcg
    16021 gcaatagcgt taaacagggc ttcacgttga tcgccattac gctggttact catgttaaaa
    16081 atttctttga gttctgggtt atgagtaaac atacggtcgt agaaatgggc ggttaacttt
    16141 ggccccgttt ccaccagtaa agggatggtg gcttttactg tagcgatggt ttgagcgtca
    16201 agcatatggt cttccttttt ttgcatctta attgatgtat ctcaaatgca tcttataaaa
    16261 aatagccctg caatgtaaat ggttctttgg tgtttttcag aaagaatgtg atgaagtgaa
    16321 aaatttgcat cacaaacctg aaaagaaatc cgtttccggt tgcaaactct ttattctcca
    16381 aagccttgcg tagcctgaag gtaatcgttt gcgtaaattc ctttgtcaag acctgttatc
    16441 gcacaatgat tcggttatac tgttcgccgt tgtccaacag gaccgcctat aaaggccaaa
    16501 aattttattg ttagctgagt caggagatgc ggatgttaaa gcgtgaaatg aacattgccg
    16561 attatgatgc cgaactgtgg caggctatgg agcaggaaaa agtacgtcag gaagagcaca
    16621 tcgaactgat cgcctccgaa aactacacca gcccgcgcgt aatgcaggcg cagggttctc
    16681 agctgaccaa caaatatgct gaaggttatc cgggcaaacg ctactacggc ggttgcgagt
    16741 atgttgatat cgttgaacaa ctggcgatcg atcgtgcgaa agaactgttc ggcgctgact
    16801 acgctaacgt ccagccgcac tccggctccc aggctaactt tgcggtctac accgcgctgc
    16861 tggaaccagg tgataccgtt ctgggtatga acctggcgca tggcggtcac ctgactcacg
    16921 gttctccggt taacttctcc ggtaaactgt acaacatcgt tccttacggt atcgatgcta
    16981 ccggtcatat cgactacgcc gatctggaaa aacaagccaa agaacacaag ccgaaaatga
    17041 ttatcggtgg tttctctgca tattccggcg tggttgactg ggcgaaaatg cgtgaaatcg
    17101 ctgacagcat cggtgcttac ctgttcgttg atatggcgca cgttgcgggc ctggttgctg
    17161 ctggcgtcta cccgaacccg gttcctcatg ctcacgttgt tactaccacc actcacaaaa
    17221 ccctggcggg tccgcgcggc ggcctgatcc tggcgaaagg tggtagcgaa gagctgtaca
    17281 aaaaactgaa ctctgccgtt ttccctggtg gtcagggcgg tccgttgatg cacgtaatcg
    17341 ccggtaaagc ggttgctctg aaagaagcga tggagcctga gttcaaaact taccagcagc
    17401 aggtcgcgaa aaacgctaaa gcgatggtag aagtgttcct cgagcgcggc tacaaagtgg
    17461 tttccggcgg cactgataac cacctgttcc tggttgatct ggttgataaa aacctgaccg
    17521 gtaaagaagc agacgccgct ctgggccgtg ctaacatcac cgtcaacaaa aacagcgtac
    17581 cgaacgatcc gaagagcccg tttgtgacct ccggtattcg cgtgggtact ccggcgatta
    17641 cgcgtcgcgg cttcaaagaa gcagaagcga aagaactggc tggctggatg tgtgacgtgc
    17701 tggacagcat caatgatgaa gccgttatcg agcgcatcaa aggtaaagtt ctcgacatct
    17761 gcgcacgttt cccggtttac gcataagcga aacggtgatt tgctgtcaat gtgctcgttg
    17821 ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aaactttgca aattcaatat
    17881 attgcaatat ccgtgtaggc ctgataagcg tagcgcatca ggcaatctta ggtatgtgat
    17941 catcaaggct tccttcggga agcctttcta cgttattgcg ccatcaaatc tgtcgtaact
    18001 gcgcctcaac atacaaatag ccaatcccca acacctgttg tgcgcggctt aattgcccaa
    18061 agccaatttg cgttgctttc acgctcggcc cttcgtcgcg gtcgaacggg ttaaatcccg
    18121 tctggcgaat aatggtatta agccagtgct caccaaaggc acaactgcga ccgtacaacc
    18181 aaatctgatt aatattgagg atgttgagga agttatacag actcaaacca atggcattgg
    18241 cactgcgatc aacccaactg gtgatccacg gttctccact ctaccaggca gcgattaact
    18301 gcgctgtagt cagtttttcc ggatcaagct gagtattaac cggttgtgat tttagccata
    18361 cccgcgcctg tttttttaat gcacttaacg aggcgacggt ttccaggcag ccgtaacgtc
    18421 cacagtcgca gacgacgcca tccggattaa cgatggtgtg actaatctgt ccgctaccat
    18481 acaaactgcc gcgataaatt tgctcgttaa tcacgaacga cgagccaatg ccgtaatcaa
    18541 cgttgatcac gcagaaatcc cgtttctgcg aattattttg ccatttctcc gccagcgcca
    18601 gcatcacgca gtcattatcg accatcaccc gaatgccgag cttctcttcc agcagatact
    18661 tcacctcaac cggcgttgtc cacggcgctt gcggcatggt ttgcgacacg ccggtcaccg
    18721 gatcaacctg accgtggatt gccagcgcca gattgatggt gcggtccggc cacaatttac
    18781 ggtggcgatg ccagcatttt tcgatttcgg acagcagcgc ctgcggcgtc ggtgcatcaa
    18841 tctgtaaata ctcaaattca cctttcggac ttaaacaagc attagcaacc tgacactcaa
    18901 tactggtggg cgtcacgttc aggcacagcg tccagtcacc ttccggcgca atcagccatg
    18961 taccgctact atgcccgcgc gtctggcttt catcgtcgat attcaccacc cgtttttcgc
    19021 tttccagttc ctgcaaaata ttactgactg ccggaatcga aatttgcgcc agccgggcca
    19081 gcgttgattt attggcgcgc ttttgccggt acagcagttc cagaatcacg catttgttat
    19141 ggtggcgaat ctgttgattg ttaatgcagg ctctcatctt cactaaacct tgtttagtta
    19201 attgcgtgat tattccgcga aaacattgcc agattgttca atacactgcc acaaatcttt
    19261 taattcagta tgtcttgtta atattgaggg caccatgact ccagtaaaag tgtggcaaga
    19321 gcgcgttgag atcccgacct atgaaaccgg gccgcaggat atacatccca tgttcctgga
    19381 aaatcgcgtt tatcagggat cgtccggcgc ggtttatccc tacggcgtga ccgatacgct
    19441 gagcgagcag aaaaccctga aatcctggca ggcggtgtgg ctggaaaacg actacatcaa
    19501 agtgatgatc ctgccggaac tgggcggtcg ggtgcatcgc gcgtgggata aagtgaaaca
    19561 gcgcgatttt gtttatcaca atgaagtcat taaacctgcg ctggtggggc tgctggggcc
    19621 gtggatttcc ggtgggattg agtttaactg gccgcaacat catcgcccga ccacctttat
    19681 gcccgttgat ttcaccctcg aagcccatga agacggtgca cagacggtgt gggtaggcga
    19741 aacggagccg atgcatggtt tacaggtgat gacaggtttc accctgcgcc ctgggcggcg
    19801 ctggaaatcg ccagccgcgt ctataacggc aacgccacgc cgcgtcattt cttgtggtgg
    19861 gccaacccgg cagtgaaagg gggtgaaggg catcagagcg ttttcccacc ggatttgact
    19921 gcggtgtttg atcacggcaa acgggccgtc tccgctttcc ccatcgctac cggcacttac
    19981 tacaaagtgg actactccgc tggagtggac atttctcgct ataaaaatgt gcccgttcca
    20041 acctcatata tggccgaaaa atcgcagtac gattttgtcg gcgcgtggtg tcacgatgaa
    20101 gatggcggtt tgctgcacgt tgccaaccac catattgcgc caggtaaaaa acagtggagt
    20161 tggggacaca gtgaatttgg ccaggcgtgg gataagagtc tgaccgacaa taacggcccg
    20221 tatatcgaac tgatgaccgg tatttttgcc gataaccagc ccgattttac ctggcttgat
    20281 gcttacgaag agaagcgttt tgagcagtat ttcctgcctt atcattctct gggcatggtg
    20341 caaaatgcct cccgcgatgc ggtgataaaa ctccagcgta gtgagcaggg gattgagtgg
    20401 gggctgtatg ccatctctcc gttgaacgga taccgcctgg cgatccacga aatcggcaaa
    20461 tgcaacgcgt tacttgatga tgccgtggca ctgatgcctg cgaccgccat ccagggcgtg
    20521 ttgcacggta tcaatcctga taggctgacc attgagcttt ccgatgccga cggcaatatt
    20581 gtactgagtt atcaggaaca tcagccgcaa gcgttgccgt taccggacgt cgccaaagcg
    20641 ccactggcag cacaagacat taccagtaca gatgaagcct ggtttatcgg tcagcatctg
    20701 gagcaatatc atcacgccag ccgttcaccg ttcgattact acctgcgcgg cgtggcgctg
    20761 gacccgctgg attatcgctg taacctggcg ctggcgatgc tggaatataa ccgcgcagat
    20821 ttcccgcaag cggtggcgta tgccagtcag gcgctgaaac gcgcacatgc gctgaacaaa
    20881 aacccgcagt gtggacaagc aagtttgatt cgcgccagtg cttacgaacg tcagggacaa
    20941 tatcaacaag ccgaagagga tttctggcgt gcggtctgga gcggcaacag caaagccggt
    21001 ggctattatg gcctggcacg actggcggcg cgtaatggta acttcgacgc tggtctggat
    21061 ttttgccaac aaagtcttcg cgcctgccca accaatcagg aagtgctttg cctgcataat
    21121 ctgctgctgg tgttaagtgg tcgtcaggac aacgcgcgtt tgcagcgcgc gaaactgctg
    21181 cgtgattatc cgctgaacgc cattctgtgg tggctgaact ggttcgatgg tcgtagcgaa
    21241 tcagccctcg cgcagtggcg cggtctgtgt cagggacgcg acgttaacgc cctgatgacc
    21301 gccgggcaac tgattaactg gggaatgccc actctggcga cagagatgct gaacgcactg
    21361 gactgtcagc gcacgttatc gctttatcta caagccagct tgctgccgaa agccgaacgc
    21421 ggcgaactgg tcgcaaaagc cattgatgtc ttcccgcagt ttgtccgttt cccgaatacg
    21481 ctggaagaag tggcggcgct ggagagtatt gaagagtgct ggtttgcccg tcatttactg
    21541 gcctgtttct actacaacaa gcgcagctac aacaaagcca ttactttatg gcaacgttgc
    21601 gtagagatgt cgccggagtt tgccgatggc tggcgcgggt tggcgatcca tgcgtggaat
    21661 aagcaacacg attatgaact ggccgcgcgt tatcttgata atgcttatca gcttgcgccg
    21721 caggatgcac gtctgctttt cgaacgggat ttgctggata agttaagtgg agccacaccg
    21781 gagaaacgac tggcgcgtct ggaaaataat ctggaaattg cgctgaaacg cgacgacatg
    21841 accgcagaac tgctcaattt gtggcatctc acgggtcagg cagacaaagc ggcggacatt
    21901 ctcgccacgc gcaaattcca cccgtgggaa ggcggggaag ggaaggtcac cagtcagttt
    21961 atcctcaacc agttattacg cgcctggcag catcttgatg ccagacagcc gcagcaggcc
    22021 tgcgaactgc ttcatgccgc gctgcattat ccggagaatt taagcgaagg ccgtttaccg
    22081 gggcaaactg ataacgacat ctggttctgg caggcgatat gcgccaacgc gcagggcgat
    22141 gaaactgaag cgacgcgttg tttacgtctg gcggcgaccg gcgatcgcac cattaacatc
    22201 cacagttatt acaacgatca gccggttgat tatctcttct ggcaaggaat ggcgctgcga
    22261 ctgctgggcg aacaacaaat cgcacagcaa ctgtttagtg aaatgaaaca gtgggcgcaa
    22321 gagatggcga aaaccagtat tgaggcggat ttctttgctg tttcacaacc tgacctgttg
    22381 tcgctgtatg gcgatttaca acagcagcat aaagaaaaat gcctgatggt ggcgatgctg
    22441 gcgtccgcgg gactcgggga ggttgcgcaa tatgaatctg ctcgcgctga attgacggcg
    22501 attaatccgg cctggccgaa agcggcatta ttcaccaccg tgatgccttt tatttttaac
    22561 tacgttcact aaaccctatt aactgtaata tttgagcggc acgcgctgcc gctcatcaca
    22621 aaataattag aataagccgt tgttaataaa aactctacag gaggcactat gcctaaaaaa
    22681 atgagaacaa cccgtaatct attattaatg gctacgctat taggtagcgc actatttgcc
    22741 agggctgcgg ataaagaaat gaccattggt gcaatatacc ttgataccct gggatattac
    22801 gctggagtgc gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca ggtacagtta
    22861 attgaaacta acgcccaggg tgatatttcg aaagaaagta cctttgttga taccctcgtg
    22921 gcgcgtaatg tcgatgccat tattttatcg gcagtgtctg aaaatggcag tagccgtacc
    22981 gttcgtcgcg ccagtgaagc gggtattccg gtgatttgct acaacacctg tattaatcaa
    23041 aagggtgtcg ataaatatgt ctcggcgtat ctggtcggcg atccactgga atttggtaaa
    23101 aaactgggta acgctgccgc cgattatttt attgccaata aaattgacca gccgaaaatt
    23161 gccgtcatca attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa
    23221 gtattaaaat cccgcgttcc cggcgcgcaa attgtcgcta atcaggaagg gactgtttta
    23281 aataaagcga tttccgttgg tgaaaaactg attatctcca cgccggatct caacgccatt
    23341 atgggggagt caggcggtgc gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag
    23401 gccggaaaaa ttgctgtttt cggttcggat atgacaaccg aaattgctca ggagctggaa
    23461 aacaatcagg tgctgaaagc ggtagtggat atttccggta agaaaatggg caatgctgtt
    23521 ttcgcgcaaa cattgaaggt tatcaataaa caagccgacg gtgaaaaagt gattcaggtg
    23581 ccaatcgatc tctataccaa aacggaagat ggtaaacagt ggctggcaac gcacgttgat
    23641 ggtctgccct aatcgctgat tcgcagaggt ccctatgttc acggcaacag aggcagtccc
    23701 ggtagcaaaa gtggtggcag gaaataagcg ttaccccggc gtcgttgcgc tggataacgt
    23761 taacttcacg ctcaataaag gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa
    23821 atcgactctc attcgaatgc ttaccggcag cgaacgcccg gatagcggtg atatctggat
    23881 tggcgagacg cgactggaag gtgacgaagc tacgttgact cgccgtgccg ctgaactggg
    23941 ggttcgtgcg gtttatcagg aattaagtct ggtggaaggg ctgacggtgg cagaaaacct
    24001 ctgccttggt caatggcctc gccgcaacgg catgattgat tacctgcaaa tggcgcagga
    24061 tgcccaacgt tgcttacagg cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac
    24121 gctaagcccg gcgcaaaagc aactggtgga aattgcacgg gtgatgaagg gcgagccgcg
    24181 cgtggtcatt cttgatgaac ccaccagctc gcttgccagt gcagaagttg aactggtgat
    24241 cagtgcggtg aaaaagatgt cggcactggg cgtggcggtg atttatgtca gccaccggat
    24301 ggaagaaatc cgccgcattg cctcctgtgc caccgttatg cgcgatggtc aggtggcggg
    24361 cgatgtgatg ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc tagggcgtga
    24421 tcacgttgat attgcgccgg ttgcccctca ggaaattgtg gatcaggccg tactggaagt
    24481 ccgtgcgtta cgccataagc ccaagctgga ggatatcagc tttacgttac gtcgcggcga
    24541 agtgcttggc attgctggcc tgctgggggc agggcgcagt gaattgctga aggcgattgt
    24601 tgggctggag acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa tcacgcgccc
    24661 cgattacggc gacatgctga aacgcggcat tggctatacg ccagaaaacc gcaaagaagc
    24721 ggggatcatt ccctggttgg gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat
    24781 cagcgccaac ggagtgctgc aatggtccac catccgccgc ctgaccgaag aggtgatgca
    24841 gcggatgacg gtcaaggccg ctagtagcga aacgcccatc ggcacgcttt ctggtggcaa
    24901 tcagcaaaaa gtggtgatcg gtcgttgggt ctatgccgcc agccagattt tgttgctcga
    24961 cgagccaacg cgcggcgtcg atatcgaagc caaacagcag atttaccgta ttgtccgtga
    25021 gctggctgcc gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg agttgccgct
    25081 ggtgtgtgac cgcatcctgt tattacagca cggcacgttc tcgcaggagt ttcactcgcc
    25141 ggtcaatgtg gatgagctga tgtccgccat tctgtctgtg cactgatgaa ttttaccgag
    25201 gaacctgaag atgtctgctt cgtcattacc attgccgcag ggcaagagcg tctcgctcaa
    25261 acaatttgtc agtcgccata ttaatgagat cggtttgctg gtggtgattg ccattcttta
    25321 tctggtcttc tccctgaacg cgcctggctt tatctcattg aataaccaga tgaacgtgct
    25381 gcgcgatgcc gccaccattg ggattgccgc ctgggcgatg acgctgatta ttatctccgg
    25441 tgaaattgat gtcagcgttg ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt
    25501 gctgcaattt gaagttccgt tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct
    25561 gatgggaacg ctcgccgggg tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac
    25621 actggggctg tggagcgccc tgcgcggaat ggggctgttt atgaccaacg cactgccagt
    25681 gccgattgac gaaaacgagg tgctggactg gctgggcgga caatttctcg gtgtgccagt
    25741 atccgcgctg atcatgatgg tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc
    25801 cttcgggcgc tcggtttttg ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat
    25861 taacgttcgt cgggtgcgca ttcttatctt taccctttcg ggattattag cggcggtgac
    25921 cggcattttg ctggcggcac gcctcggttc cggtaacgca ggtgccgcaa acggtctgga
    25981 gtttgacgtc atcgccgcgg tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc
    26041 cctgttcggt acattgcttg gtgtgctggt gattacgcta atcggtaacg gtctggtgct
    26101 gctcggtatt aactcctttt tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt
    26161 gctggcgaat atcttgctga cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc
    26221 cctggcgcgg gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa
    26281 acgatgctgg cagcttattt accaggaaat tcaaccgtcg atctgcggga agttgcggtg
    26341 ccgacgccgg ggattaacca ggtactgatc aaaatgaaat cctccgggat ttgcggaagc
    26401 gatgtccact atatctatca tcaacaccgt gccacggcgg cggcacccga taaaccgtta
    26461 taccagggat ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat ggggcaaggc
    26521 tgccgccatt ttaaagaggg cgaccgcgtg ctggtgtatc acatttctgg ctgtggtttt
    26581 tgcccgaact gccgccgcgg ctttcctatc tcttgtactg gcaaaggaaa agcggcttac
    26641 ggctggcagc gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa agatctgatc
    26701 ctcctgccgg atgcgctgag ctacgaagat ggtgcgttta tcagttgcgg cgttggcacg
    26761 gcctatgaag ggattttgcg cggcgaagtt tccggcagcg ataacgtgct ggtggtcggt
    26821 ctggggccgg tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc
    26881 atcggcgttg atatgctgcc ggaacgtctg gcgatggcaa aacagttagg ggtgatggat
    26941 cacggctatt tagcaaccac cgaaggtctg ccgcagatta tcgccgaact cacccacggt
    27001 ggcgcggatg ttgcgctcga ttgttctggt aatgccgcag gtcgcttgct ggcactgcaa
    27061 tccaccgctg actggggacg ggtggtttac attggtgaaa ccggaaaagt ggaattcgag
    27121 gtcagcgccg atctgatgca ccatcaacgg cggattatcg gctcctgggt gaccagtctg
    27181 ttccatatgg aaaaatgcgc ccacgatttg actgactgga agctgtggcc gcgtaacgcc
    27241 attacccatc gcttctcgct ggaacaggca ggtgatgcct atgcgctgat ggcgagcggc
    27301 aaatgcggga aagttgtgat taacttcccg gattaaggag ttagcggatg accatctata
    27361 ccttatccca cgggtcgcta aagctggacg tttctgacca gggcggcgta atcgaaggct
    27421 tctggcgcga tacgacgccg ctcttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg
    27481 cctcatgttt tccgctggtg ccatttgcaa accgggtgag cggcaaccgt tttgtctggc
    27541 aggggcgtga gtatcaactg caaccgaatg ttgagtggga tgcgcactat ctacacggcg
    27601 atggctggct gggcgagtgg caatgtgtct cgcacagcga tgatagtttg tgtctggtgt
    27661 atgaacatcg cagcggtgtc tatcactatc gggtaagtca ggcgtttcat ttaacggcgg
    27721 atacgctgac ggtgacgctc tctgtcacca atcaaggggc agagacgctg ccatttggta
    27781 ccggctggca tccttatttt ccgttgtcac cacaaacgcg gattcaggcg caggcgagcg
    27841 gttactggct ggaacgggag cagtggctgg cgggtgagtt ttgcgagcag ctaccgcagg
    27901 aactggattt taaccagctc gcgccgttgc cgcgccagtg ggtgaacaat ggttttgccg
    27961 gatggaatgg tcaggcccgc atcgagcagc cgcaggaagg ttatgccatc atcatggaaa
    28021 cgacgccacc tgcaccgtgt tatttcatct ttgtttccga ccctgcgttt gataaaggat
    28081 atgcgtttga tttcttctgt ctggaaccga tgagccatgc gccggatgat catcatcgcc
    28141 cggaaggggg tgatctcatt gcgctagcac caggggaatc aacaacttca gagatgtcgt
    28201 tgcgggtaga gtggctgtaa tttattaaat gccggataag gcgttcacgc cgcatccggc
    28261 atctttccta acgccgatcg agagaaattg cccccggccc ggtaattgcc aatagcaaga
    28321 acgcgccagc gatactgaca ttcttccaga aattaatcat atttggccca accgcatcgc
    28381 cggtcatatc ccagtaatgg tgaccaatca ccgccgtacc cagcgtgtag aaaataaaca
    28441 gcaccgccag cggacgggtg aaaaagccaa gcacgattaa tatcgcggcg ggcacttcca
    28501 taactaccgc aataatcgct gccagcatcg gcattggcgc gcccaacgag gccatatatt
    28561 ggaccgtacc gtcaaagccc atcattttgg gaaaaccaaa aataatgaaa attaagacca
    28621 cggcgatacg ggcaattaat aacaaaacgg ggcgggcagc tccaaaatca aaataacg
//
LOCUS       DAZPAC010000035        28517 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZPAC010000035 DAZPAC010000000
VERSION     DAZPAC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28517
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.035"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(285..512)
                     /locus_tag="ACHUPM_001237"
     CDS             complement(285..512)
                     /locus_tag="ACHUPM_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR0869072.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            704..1909
                     /gene="csdA"
                     /locus_tag="ACHUPM_001238"
     CDS             704..1909
                     /gene="csdA"
                     /locus_tag="ACHUPM_001238"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR0869073.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            1909..2352
                     /gene="csdE"
                     /locus_tag="ACHUPM_001239"
     CDS             1909..2352
                     /gene="csdE"
                     /locus_tag="ACHUPM_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR0869074.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2403..3209)
                     /gene="tcdA"
                     /locus_tag="ACHUPM_001240"
     CDS             complement(2403..3209)
                     /gene="tcdA"
                     /locus_tag="ACHUPM_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR0869075.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(3286..4383)
                     /gene="mltA"
                     /locus_tag="ACHUPM_001241"
     CDS             complement(3286..4383)
                     /gene="mltA"
                     /locus_tag="ACHUPM_001241"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR0869076.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            4592..4668
                     /locus_tag="ACHUPM_001242"
     tRNA            4592..4668
                     /locus_tag="ACHUPM_001242"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4626..4628,aa:Met,seq:cat)
     gene            complement(4743..5996)
                     /gene="amiC"
                     /locus_tag="ACHUPM_001243"
     CDS             complement(4743..5996)
                     /gene="amiC"
                     /locus_tag="ACHUPM_001243"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR0869077.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            6228..7559
                     /gene="argA"
                     /locus_tag="ACHUPM_001244"
     CDS             6228..7559
                     /gene="argA"
                     /locus_tag="ACHUPM_001244"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR0869078.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(7621..9447)
                     /gene="recD"
                     /locus_tag="ACHUPM_001245"
     CDS             complement(7621..9447)
                     /gene="recD"
                     /locus_tag="ACHUPM_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR0869079.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKRRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(9447..12989)
                     /gene="recB"
                     /locus_tag="ACHUPM_001246"
     CDS             complement(9447..12989)
                     /gene="recB"
                     /locus_tag="ACHUPM_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR0869080.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(12982..15870)
                     /gene="ptrA"
                     /locus_tag="ACHUPM_001247"
     CDS             complement(12982..15870)
                     /gene="ptrA"
                     /locus_tag="ACHUPM_001247"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR0869081.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(16046..19414)
                     /gene="recC"
                     /locus_tag="ACHUPM_001248"
     CDS             complement(16046..19414)
                     /gene="recC"
                     /locus_tag="ACHUPM_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR0869082.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(19427..19750)
                     /locus_tag="ACHUPM_001249"
     CDS             complement(19427..19750)
                     /locus_tag="ACHUPM_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR0869083.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(19735..20142)
                     /locus_tag="ACHUPM_001250"
     CDS             complement(19735..20142)
                     /locus_tag="ACHUPM_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR0869084.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(20139..20702)
                     /locus_tag="ACHUPM_001251"
     CDS             complement(20139..20702)
                     /locus_tag="ACHUPM_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR0869085.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(20693..21163)
                     /locus_tag="ACHUPM_001252"
     CDS             complement(20693..21163)
                     /locus_tag="ACHUPM_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR0869086.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(21348..22142)
                     /gene="thyA"
                     /locus_tag="ACHUPM_001253"
     CDS             complement(21348..22142)
                     /gene="thyA"
                     /locus_tag="ACHUPM_001253"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR0869087.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(22149..23024)
                     /gene="lgt"
                     /locus_tag="ACHUPM_001254"
     CDS             complement(22149..23024)
                     /gene="lgt"
                     /locus_tag="ACHUPM_001254"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR0869088.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(23175..25421)
                     /gene="ptsP"
                     /locus_tag="ACHUPM_001255"
     CDS             complement(23175..25421)
                     /gene="ptsP"
                     /locus_tag="ACHUPM_001255"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR0869089.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(25434..25964)
                     /gene="rppH"
                     /locus_tag="ACHUPM_001256"
     CDS             complement(25434..25964)
                     /gene="rppH"
                     /locus_tag="ACHUPM_001256"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR0869090.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            complement(26317..26463)
                     /gene="ygdT"
                     /locus_tag="ACHUPM_001257"
     CDS             complement(26317..26463)
                     /gene="ygdT"
                     /locus_tag="ACHUPM_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR0869091.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            26649..27338
                     /gene="mutH"
                     /locus_tag="ACHUPM_001258"
     CDS             26649..27338
                     /gene="mutH"
                     /locus_tag="ACHUPM_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR0869092.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            27407..28120
                     /locus_tag="ACHUPM_001259"
     CDS             27407..28120
                     /locus_tag="ACHUPM_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0869093.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            28258..28476
                     /gene="ygdR"
                     /locus_tag="ACHUPM_001260"
     CDS             28258..28476
                     /gene="ygdR"
                     /locus_tag="ACHUPM_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR0869094.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT         6811 a         7789 c         7143 g         6774 t
ORIGIN      
        1 ttaatctgag ccattataaa ttgtccgttg agcttctacc agcaaatacc tatagtggcg
       61 gcacttcctg agccggaacg aaaagtttta tcggaatgcg tgttctggtg aacttttggc
      121 ttacggttgt gatgttgtgt tgttgtgttt gcaattggtc tgcgattcag accatggtag
      181 caaagctacc ttttttcact tcctgtacat ttaccctgtc tgtccatagt gattaatgta
      241 gcaccgccta attgcggtgc ttttttttac cttgcgatcg cgaattactg atccagttcg
      301 accatctctt tcacgtcagt acggttgatc tgctgtttgt tgccattagc gtctttatac
      361 gaaatcatac cggtatcgtt atcagtctgt ggtttgccgt cagagacgat ggtacgtccg
      421 tcattggtgt gcatcacata gttcgaaccg gaacaggcgc tcagggcaaa agtcagcata
      481 caggcagaaa taattgcggc agtctttttc atcatcttct ccttaaagcg attaataaat
      541 cttaatagta ttgattaaca ggctaaaatt aacgcctaac actattcagc atatgttact
      601 tacgcggctt cgccaggata tccagataat tctgatggtt agcactctcc ttgtatcaaa
      661 gtgaattttg cgtcacgatc ggtgcatcaa gccgaggagt accatgaacg tttttaatcc
      721 cgcgcagttt cgcgcccagt ttcccgcact acaggatgcg ggcgtctatc tcgatagcgc
      781 cgcgaccgcg cttaaacctg aagccgtggt tgaagccact cgacagtttt acagcctgag
      841 cgccggaaac gtccatcgca gccagtttgc cgaagcccaa cgcctgaccg cgcgttatga
      901 agctgcacga gagaaagtgg cgcaattact gaatgcaccg gatgataaaa ctatcgtctg
      961 gacgcgcggc accactgaat ccatcaacat ggtggcacaa tgctatgcgc gtccgcgtct
     1021 gcaaccgggc gatgagatta ttgtcagcgt ggcagaacac cacgctaacc tcgtcccctg
     1081 gctgatggtc gcccaacaaa ctggagccaa agtggtgaaa ttgccgctta atgcgcagcg
     1141 actgccggat gtcgatttgt tgccagaact gattactccc cgtagtcgga ttctggcgtt
     1201 gggtcagatg tcgaacgtta ctggcggttg cccggatctg gcgcgagcga ttacctttgc
     1261 tcattcagcc gggatggtgg tgatggttga tggtgctcag ggggcggtgc atttccccgc
     1321 agatgttcag caactagata tcgatttcta tgctttctct ggtcacaaac tgtatggccc
     1381 gacgggtatc ggcgtgctgt atggcaaatc agaactgctg gaagcgatgt cgccctggct
     1441 gggtggcggc aaaatgattc acgaagtgag ttttgacggc ttcacgactc aatctgcgcc
     1501 gtggaaactg gaagcaggaa cgccaaatgt ggctggtgtc ataggattaa gcgcggcgct
     1561 ggaatggctg gcagattacg atatcaacca ggcagaaagt tggagtcgca gcctggcgac
     1621 gctggcggaa gatgcgctgg caaaacgtcc tggcttccgc tcattccgct gccaggattc
     1681 cagcctgctg gcctttgatt ttgctggcgt tcatcacagc gatatggtga cgctgctggc
     1741 ggagtacggt attgccctgc gggccgggca acattgcgct cagccgctac tggcagaatt
     1801 aggcgtaacc ggcacactgc gcgcctcttt tgcgccatat aatacaaaga gtgatgtgga
     1861 tgcgctggtg aatgccgttg accgcgcgct ggaattattg gtggattaat gacgaacccg
     1921 caattcgccg gacatccgtt cggcacaacc gtaaccgcag aaacgttacg caataccttc
     1981 gcaccgttgt cgcaatggga agataaatat cgccagttga tcatgctggg gaaacagctt
     2041 ccggctttgc cagacgagtt aaaagcgcag gctaaagaga ttgccggatg cgaaaaccgc
     2101 gtctggctgg gatataccgt gactgaaaac ggtaaaatgc acttctttgg cgatagcgaa
     2161 gggcgcattg tgcgcggcct gctggcggtg ctgttgactg ccgttgaggg gaaaaccgcc
     2221 gccgaattgc aggcacagtc gccgctggca ttgtttgatg agctgggatt gcgtgcgcaa
     2281 cttagcgcct cacgcagcca ggggttaaat gcgttaagcg aggcgattat cgctgcgacg
     2341 aagcaggttt aagcatagaa ccgggtcgga taagacgttc gcgtctcatc cgacctgatt
     2401 gtttaaccct gacgcgccgc tttcgccatc atcttcttca gcgcatgaga aaccgcaaca
     2461 aacccaaagg tggcggtcac catcgttgcc gcgccaaatc cagatgcaca atccatccgc
     2521 ttcggccctt ctgccgtggc tttcatcgca cacacagtac cgtctgactg cgggtacacc
     2581 agcgcttcag tagaaaacac gcaatcgacg ccgagcttac ctttactgtt tttcactacg
     2641 ccaaaatcgc ttttcaggcg ctcgcgcaac ttcgccgcca gcggatcctg aatcgttttc
     2701 gccagatcgg taacctgaat ctgcgtcggg tcaatctgcc cacccgcgcc accggtcgtg
     2761 accagcggga ttttattgcg ccgacaataa gcaatcagcg ccgctttggg ccgtacacta
     2821 tcaatggcat caatcacgta cgaataaccc acgctcatat actgcgctac gttatccggc
     2881 gtcacgaaat catccaccac cgttacacgg cactccgggt taatctggcg aatacgctcc
     2941 gccatgactt ccgcttttgc cagcccgacg ttatcgcgca gagcgtgaat ttgccgattg
     3001 gtattggtga cgcacacatc atccatatcg ataagcgtga ttgcgccaat ccccgtgcgc
     3061 gccagcgctt ccgccgccca ggaaccgaca ccaccgatac caaccacaca aatatgcgcg
     3121 tcagcaaaca gttgcaacgc tttttcacca tacagacgcg ctgtgccacc aaaacgctga
     3181 cgccatgcat cactaattac cacagacata gcacctcaga attaaaaagg gtgaggttat
     3241 ccctcaccct gtcgtatccg taaaaacggt atacagaata acacatcagc cgctaaagac
     3301 gttacctgcg cccggggcgg ttttcagcac ccagacacgt ccatagtggt tgtaccaacc
     3361 tgcgcggtgt ccggcttccg gcccgatccc ttgatagata tcgaagtgtt ggcctttgat
     3421 tgcaccaccg acatccagcg ccaccatcag acgcagttcg tactgaccat taaatttgcc
     3481 gttattatcc agcaacggca cttctgccag caaggtagta cctggcggaa taatggaacg
     3541 atcagaggca actgacgcgc gaccaaccag cggcaccgca cttgcccctt tcaccggtgc
     3601 aaaagattgc ggtttaaaga agacgaaaga cgggttctgt tccagcagct cgcgaacctc
     3661 ggcttcactg tgtgtttcgc cccagtgacg aatcgcctgc atcgacatat cttctttttt
     3721 cacttcgcca cggtcgatca gcaccttacc aatgctgcga taggcatgac cgtttttccc
     3781 tgcatagctg aaaaagttaa gcggactgcc atcaccaaag tcgatatacc cactaccctg
     3841 cacatccata atgaagttat ccatcaggga gttactgtaa gcgagaatat atttatcact
     3901 caatgccccc gcgtagatct ccgcacgaga cggcagacga ccacgttttg gcggcatacg
     3961 gtaaatagga tactggaact cgccctggcg ggtatggcgc gcctgaatta ccggcgtgta
     4021 atagcctgta aactgcacgt taccatagtt gtcggcacct tccatctgcc aggcatcaat
     4081 gccgaactgg cgcatattgc gggtatcacc gcctgcgcgc agccactctt gcaccgcgtt
     4141 ataaacatta ctctggttgc catacagacg cggtgacgaa ttacggatat ggttaatttg
     4201 ctcggcaaaa tcaccggcgt taatcggcgc gccaacggca tctggctggt tcaccagaga
     4261 gaaaggctgg gtaaatttcc cgtctttata ttgctgtccg cgatcggttg gtttggaaga
     4321 gcaggcggca agcattgcca caaccgtgcc cataagaagg tactttaccc aacgtccttt
     4381 cattgttctc ttcttcaggt taaaaaataa ggcgcaacga agataacaaa ccgccgcgtt
     4441 taatgaaatg agcagcctct ccctgacgca aattttgcac aaaaaatagg ctttagtgat
     4501 ttgtttttgt tcaaaatcat gccagatccg tgatcggggt aaaaaaaagg ttgcatgaaa
     4561 acgcgagcgg agtatagtgc gcatccacgg aggcggggtg gagcagcctg gtagctcgtc
     4621 gggctcataa cccgaaggtc gtcggttcaa atccggcccc cgcaaccaat tattgaacac
     4681 cctaacgggt gtttttttgt ttctactctc ccataaaaaa agcgccattc agcgcctttt
     4741 tatcatcccc ttctcgccag cgttgcccca tcggcaaaat acgctttaat ccccgcaaga
     4801 atagactccg caacttcctg ctggaaagtc gccgttttca gtttacgctc ttcctcaacg
     4861 ttactgataa acgccgtttc gaccagaatg gagggaatat ctggtgcctt tagtacggca
     4921 aacccggcct gttcaacttg atttttatgc agcttgttga ttttaccgag cttattcagc
     4981 actgctttac caaacttaag gctgtcagca atggtcagcg actgcaccat atcgaacatg
     5041 gtgtggtcga catagcggtc accgcttttg ctcacgccac caatcaagtc cgaggcgttc
     5101 tgggtttgtg ccagatattt tgccgcagta ctggttgcac ctttggttga gagcgcaaac
     5161 accgaggaac cgctcggctg gcgactggta aaggcgtcgg catggataga gacaaacagg
     5221 tcagcacgct gtttctgggc ttttgctacg cgcacttgca acggaatgaa gatgtcttca
     5281 ttgcgcgtca tgtacacctt catattgccc tctttctcga tcagagagcg caaacggcga
     5341 gctatttgca ataccacatc tttttcgcgc gttttgtatt tccccaccgc accggagtct
     5401 tcgccaccgt ggccagggtc aagcataatg acaatcggac gatcgcgccc tgctttacct
     5461 ggttgtggac cactttgcgc tggcggcacc tgcttttcga ggtcgccttt gttgtaatcc
     5521 tccagcagcg ccagcagcgg gtcctgcata tcctgtgcat tggccggata gaggtccatc
     5581 accagacgct ctttaaaccc ggcgaccggc gcaagggcaa acagctgcgg ttttacgttt
     5641 tgctttaatt caaaaaccat ccgtacggtt tgcgggtcaa attgcccgac gcgcgccgac
     5701 ttgatgaacg ggtcgtctgc acgaatttgt gccgccatcc ccttgagtac cgagttcagg
     5761 tttacatctt cgatatccac caccacgcgt tcagggttac tcaacgcgaa ctgcttatat
     5821 ttcagctgac gatttgattc taccgtcacg cgggtgtagc tggacgcagg ccagacgcgc
     5881 accgccacga cctggctaac cgcagccagg ctgacctgac ttacgctcaa tagccacata
     5941 gcgcccgcgc cttgcagtaa acgacgacgg ctgattgcag tgttggatcc tgacatgcct
     6001 ctcccgagca aaagaaatct aagctgtgta acaagtaaac gactaatttg accggaaact
     6061 ttagcgaaag acgcataatc tgtcatctaa taaacggtaa acattctttt tatattcacg
     6121 gcattactga taaaaaagtc gctctcgcat aaaatttaca cttgcaccct gcgaaaaaac
     6181 agaataaaaa tacaataatt tcgaataatc atgcaaaggg gtgtgccgtg gtaaaggaac
     6241 gtaaaaccga gctggtcgag ggattccgcc attcggttcc ctatatcaat acccaccggg
     6301 gaaaaacgtt tgtcatcatg ctcggcggtg aagccattga gcatgagaat ttctccagta
     6361 tcgttaatga tatcgggttg ttgcacagcc tcggcatccg tctggtggtg gtctatggcg
     6421 cacgtccgca gatcgacgca aatctggctg cgcatcacca cgaaccgctg tatcacaaga
     6481 atatacgtgt gaccgacgcc aaaacactgg aactggtgaa gcaggctgcg ggaacattgc
     6541 aactggatat tactgctcgc ctgtcgatga gtctcaataa cacgccgctg cagggcgcgc
     6601 atatcaacgt cgtcagtggc aattttatta ttgcccagcc gctgggcgtc gatgacggcg
     6661 tggattactg ccatagcggg cgtatccggc ggattgatga agacgcgatc cgtcgtcaac
     6721 tggacagcgg tgcaatagtg ctaatggggc cggtcgctgt ttcagtcact ggcgagagct
     6781 ttaatctgac ctcggaagag attgccactc aactggccat caaactgaaa gctgagaaga
     6841 tgattggttt ttgctcttca cagggcgtca ctaatgacga cggtgatatt gtctcagaac
     6901 ttttccctaa cgaagcgcaa gcgcgggtag aagcccagga agagaaaggc gattacaact
     6961 ccggtacggt gcgctttttg cgtggcgcag tgaaagcctg ccgcagcggc gtgcgtcgct
     7021 gtcatttaat cagttatcag gaagatggcg cgctgttgca agagttgttc tcacgcgacg
     7081 gtatcggtac gcagattgtg atggaaagcg ccgagcagat tcgtcgcgca acaatcaacg
     7141 atattggcgg tattctggag ttgattcgcc cgctggagca gcaaggtatt ctggtgcgcc
     7201 gttctcgcga gcagctggag atggaaatcg acaaattcac cattattcag ctcgataaca
     7261 cgactattgc ctgcgccgcg ctctatccgt tcccggaaga gaagattggg gaaatggcct
     7321 gtgtggcagt tcacccggat taccgcagtt catcacgggg cgaggttctg ctggaacgca
     7381 ttgccgctca ggcgaagcag agcggcttaa gcaaattgtt tgtgctgacc acgcgcagta
     7441 ttcactggtt ccaggaacgt ggatttaccc cggtggatat tgatttactg cccgagagca
     7501 aaaagcagtt gtacaactac cagcgtaaat ccaaagtgtt gatggcggat ttagggtaag
     7561 cgatgaaaat cgccggatgc ggcaggcgta acactcgtgc gtcgcatccg gcaattacgt
     7621 ttatccccgc gaactaaaca acgccgccag accactgcgc cgctcagtac gagtggcgat
     7681 tgccgcactt aatatacgct catcggcata cagcgacaga cggcgacgcg cgcgggtcac
     7741 cgcggtgtat accagctctc gcgttactat cggcgtgcgt tggctcggta aaatcaacgc
     7801 cgcatggtcg aactccgatc cctgcgattt atgtaccgtc atcgcccacg tagtttcgtg
     7861 ctctggcaag cgactcggtt gcacagactt aatattgccg tccggcatcg caaaccagac
     7921 gcgcgtcccc tgcccgcgat ccagcgcaat accgatatcg ccattaaaca acccaagcgc
     7981 gctgtcatta cgggcaatca tcaccggtcg accttcgtac caacgagagt gcgaatgacg
     8041 atgaattttg cgcttctgtt gcataaactg ctcaattcgc tcattcagtc cagccacgcc
     8101 aaatggccct tcccgcaggg cgcacaaaag ttggtactca ttgaacgcct gaatgattaa
     8161 atccggctcg gcacgcgctt gcagcagatc cagataacgt ccataacccg caagagcttc
     8221 ctcaagcatc gcaatataat cttcgccgct ctgtaaaagc cgtttttcaa tatcagtaaa
     8281 atcctgctga aaaacggttt tcactgccgt tttatcacca cggttgatcg ccgcagctaa
     8341 ctgaccaatg ccagaatcgc tgccgaaacg atagcttttt tgcagcaggc agagactgtc
     8401 gcgcaaagat gccgcttctg tgccggttcc tgccggaacg tgagttcccg tcaggcggct
     8461 tagctgcccg gcacgctcgg cggtaaagcc ggcattggca taagcgcaga tatcgcccag
     8521 cacagcccca gcctcaaccg aggccagttg atcacgatcg ccgagaaaga tcactcgcgc
     8581 atgatcgggc aaggcgtcga tcagtctcga catcataggc agatcgatca ttgacgcttc
     8641 atctaccacc agcacatcaa gatgcagcgg gttaccggca tgatgacgta aacgctggct
     8701 acccggctgc gcgcccagca atcggtgcaa agtgctggca tcttccggaa tgcgtctctt
     8761 ttgttcatcg gtcagcggta actgtcgcaa agccttgccg agagattcgg ttaagcgcgc
     8821 ggcagcttta cccgttggtg cagccagacg gatacggcag cgttcgccgt cggccatttg
     8881 aattaacgct gccagcaact tcgccacggt ggtcgtttta ccggtgcctg ggccgccgga
     8941 aatcaccgag atccgccgcg tcagcgccac tgctgccgcc actttttgcc agttaatttc
     9001 atcgcttact ggaaaaagtt tgtccagggt ttgcgccagt agagcttcat caacctcaat
     9061 ggcatgattc acctcattga agaagcgcgc taccgtccgc tcgttacacc acatgcgatt
     9121 caaataaaga cgatcgccac agaggatcat cggcgtgggt tcatctcccc tgctgaccgc
     9181 ttgagaagcc agcaaggatt cttcccaatt ttgtagctca ccgatttcac tgacacaggt
     9241 cgccaacagc ggatgcgacg cctcgttatt ttccagtcgt gaaagcggca aacagacgtg
     9301 tccctctccg gcatcatgac ttaacagtgc cgccgcgagg gtgacggcag gatgttcatc
     9361 tcccgccacg gtcagggcaa attgcacatc cagcgggcgt agctgtttgt gctccacagc
     9421 ttccagtaat tgcttttgca atttcattac gcctcctcca gggtcatacc ggcaaacatc
     9481 tcatccatca gggcaatcaa cccggcgttg ggtcgggttg tgtaaatccc ttgttgcgga
     9541 tgttctttat caacgccacg caggaacaga tagataacgc cgccaaagtg acgatcatag
     9601 tcgtaatcag caatgcgatg gcgcagatag cgatgcagcg ccagggtata aagctgatat
     9661 tgcagatcat agcggtgtgc ctgcatcgct gctgccatag cctgttgggt gtaagccgaa
     9721 ctgtcttcac ccagccagtt ggatttatag tcgagcaggt aataacgccc ttcgtggcgg
     9781 aataccaggt cgataaagcc ttttaacatg ccgcgtacct gcatgaactc cagcggcggg
     9841 cagccagcgg atagcgggtc aaactggcga attagtgcat caagctggct ggcgatgagc
     9901 ggttcgctaa tcggcaggta aaactccatc tccacctgtt tatcgcggtc ggaaagctga
     9961 ctcaggctaa cacccgtttc attgagaggt gcctggagga cagccgtgat ccactcggtc
    10021 aacaccggtt cccactgcga ttcaaagccg ccgagttcca gtttttcctg tacccagttc
    10081 gggtcaaccg gctgggtaaa atcgaggtct tcaaacaaac tgtgcaagaa tgtccccggt
    10141 gacgcaccgc gcgggaactg atgcggtgtt aacgtcggtt cttcaacgac gctgaccacg
    10201 cctgcggcat caacatccag ccgtggcatc agatcctgag cgataccgtg accacgctgc
    10261 tgcaagccgg agtagctggt gacacgccag ttatcgccgg gcaatcgttg tagtgtcctc
    10321 gcattcagtt ctgcagttaa agcatcatta acctgccagg gctggttatc accagtttgt
    10381 gccgtttgcc aggcaatatc atcatcgcat aacgcttcaa tacaggtgcg aagccctgcc
    10441 gcatcttgcg gttccccttt ttgcagcaaa cgcccgagcg cactttggtg gacgtcggtg
    10501 tcaccttttt tatcgccacg acggcgcacc agcggtgcaa cgccgagact gcaatgccaa
    10561 accgaacgcg tcagcgccac gtaaagcaaa cgcagatctt ccgccagacg ttcgacctcc
    10621 gcgaggtcga cgctttctgg cgcagcatta agatccaaaa ctgcctcaaa cgagtggcga
    10681 tcgtgataaa acgcctgatc ctggacgcgg aaattggtaa taaatggcaa ccagaccagc
    10741 ggatattcca gccctttcga tttatggatc gtgacaatct gcaccagatg tttatcactt
    10801 tcgaggcgca tttgttggct ggaggcatta ctgtctggct cgaggatatg ttgcgataac
    10861 cagcgtacca gcgcatgttc actttccagc tgcgttccgg cttcttgaag cagttcgctg
    10921 atatgcaaga tatcggtaag acgccgctca ccgcctgccg ttgccagcaa gttttctgca
    10981 atgttacgcg ccgacatcag cgcccgcagc atcggcataa cgccacgttt gcgccagatt
    11041 tgccgataac catcgaactc ttcgactacc gcatcccacg catgttcgtc attattcagc
    11101 gtttcaatat ccagcgcatt cagccccatc attgacgttg ccagcgcact gcgcagggtg
    11161 ttctcacgtt cgggcgtcat caccgcctgc aacagccaaa gcatttcctg cgcttccaga
    11221 gtttcaaaaa cactatcgcg gttcgaaagg taaacggaag ggatttccag caacgttaag
    11281 gcatcgcgca cctgggcggc ctcctggcgg ctgcgcacca gcacgctgat gtccgaagca
    11341 cgcaccggac gcgcgtcgtc gccgttcatc agcaacgctt cgccccgctg tccggcttgt
    11401 agccagtcgc ggatttgcgc agcacatacc tgcgccatgg tactttgata gtcgccaacg
    11461 ccgcagcttt cgccttccat cagccacatt ttcatcgcag gctgcgtttc acctttaaag
    11521 acaaaacgta acgcctgatt tttcccggct gatttcactg gaataaacgg tatttcgcga
    11581 aacatgaacg cgtcatcagt ctggctgaaa agcttgttca cactgttcac cattccaggt
    11641 gcggagcgcc agttggtgtc taaagtgtag tgggcgtgaa cttcactacg cgccttcata
    11701 taagtgaaga tatccgcacc ccggaatgca tatatggcct gcttcgggtc gccaatcagc
    11761 aacaatgcgg tttctggctg atggtgccag atacggcgaa aaattcggta ctgctggggg
    11821 tcggtatcct gaaattcatc gatcattgcc accgggaatc gcgtacggat cgccgctgcc
    11881 aacacctcgc cgctttcgct acgcagcgcg gaatcgagcc gacttaacat gtcatcaaaa
    11941 cccaattcgc cacggcggcg tttttcacgc gctactgttt cgcggatctc agccaatgcg
    12001 cgggtgatca ccagatcacg gatcgacaat ggttctgcaa gcagttgatc gatcgcctca
    12061 aacagtggat gtcgcggggt ttcccccccg gctttcgtgc gatcttctaa gaaacgctgg
    12121 gagaattttt ccagcgactc cggcaactga taactgtttg tctcttcttc tgcccaggcg
    12181 ctgatcttgt cgatccattt agcctgattg ctacggttaa acttgcgtcg atcaatacca
    12241 gaagattcga tcagcgcatc cagttcaccc actgcgtcgc gccactgctg ttttaccgta
    12301 tcaatacgcg ccacaatttg cgcgtgacgg gaagctagcg tttcatcatc gggcggcggt
    12361 gctttgataa ccggcgcttc gccttgcaga taacgattaa tatcgcgcag caacgcctgc
    12421 ggccctttcc aggtttcaaa gacgacctgg gctatttcac gcggcagcgg gtagcagtgg
    12481 cgacgccaga aatcggcgca ggcctggtag cgtagcagag actcatcttc aatcagctgc
    12541 tgctcaaaca gcatgccgga ttcaaaggca ttcaggttga gcatgcgctg gcaaaagccg
    12601 tgaatagtaa agactgccgc ttcatccatc tgccgttcgg ctaacaacaa ccactgcgcg
    12661 gcttgcgctt tatcgtcgac ctcttccagc aggtgttcgt acaatggatt gtcggtggtt
    12721 tcacgcagac aggcgatgcg caactcgtgg atattgctac ggatacgacc gcgcaattct
    12781 gccgtggcag cctcggtaaa ggtgaccacc agcagttctt caacggtcag cgggcgggga
    12841 aaggcggcgg aaccgcctag tccaagtaac aggcgcaaat aaagcgccgc aatcgtaaag
    12901 gttttgcctg tgccggcaga ggcttcaatc aggcgctcgc cctgtaaggg caagcgcaaa
    12961 ggatctagtg tctcggcgac atcactcatt cttttcactc atcaggggca ttgtttgctg
    13021 caacgcgctg acgttctccc acactttcca gccttcagga tgcacatatt cggctttccc
    13081 gttttggctg ccggaaatct gcgacaggat agccatgcct tgcggctcga ctaccgcctg
    13141 atggaagaaa tcggccagtt tttgcggcgt cagcagttta atctgggcca cgattttatc
    13201 acgcgaatcg aagcgcatat tgccgcgatc gaaatcttta cttaacttcg atgcttcttc
    13261 gccgagcgtt tgcggtgcct gcagcatctg ggtaattacc gcctgctgga tttgcgcaaa
    13321 ctcatctggc ttcatcgctc gcaattttgc ctctgcggtt gggaaaaacg ccttgtaacg
    13381 ctcccacaag aatgaaggct gtttatcatt gctttgcaaa aggaagccca tgccccactg
    13441 acgccccacg ctcattggaa acgcaaacac ggcatagccc aattgttctt cggtacgcaa
    13501 ctgattgtag aaccacggct gtacgatctg ccccaacaga gagctatagg ctgagctggt
    13561 gtattcatcg tagccagtcg gtacaaatac cgctgccagt gcggagtcgg tgctgttacc
    13621 ggctttttca aagatgacgg attgtttttt atcgaccact acatctttgt ttcgacacca
    13681 ctctgaacca tcagcgccca actgtttttg cacatcgcgt gccagcgttg ttgcctgggc
    13741 ctcggtcatg ttgccgataa ccataaactc tggtcgagcc cctgatttta aggcgtcgcg
    13801 ataagccagc acctctttca acgtaatgga gggcaaaatt ttacgccgtt catctcgcga
    13861 gaagtacggc acttgcgaga gcatctgcgc gggcataatc gcctgctcaa acgctttacc
    13921 cttttctgcg gaatccatca tctggttata ccaggacttc gcctgctcaa gctgatcttc
    13981 cgtagcggta tagctaaagt acccctcgag caatgcctgg aacagctgcg gcagacgctg
    14041 ggtgtaacca ttagcattaa ccataaggcc gttgttagcg ttggtggaaa aacttatgcc
    14101 accaaccgac gcctggttgc ttaactgatc aagcgccagc cctgcgagat aatcattgag
    14161 cgcaaacatc acctgattgc gggcgctgtc catggctttc ggattacgca aaatcaggct
    14221 gacatcagct ttgggttcgc tggaaaaata acggcttggc gcatacacca cgcgcagatt
    14281 cgactcatca acaatcagct ctggatggtc atatttcttc tctgacttaa tcagcgagaa
    14341 atcgtcagga atataggggt taagctccgg taaggagagc gcaatattgg cagctttttg
    14401 ctgccagtca gcgaaagttt gttcgctgat tttatcgacc tgatacggcg catcgacaaa
    14461 ataagccgtt ttattgtgcg gctctttcgg gctgatatac cagatacgcg cattctgcgg
    14521 cgtcatcatc gccagacgtt cttttactgc tttagcatcg taccgatcgg caatattgac
    14581 tgcatccagc gtatgctcaa ccggaacgcg aatcatggta tctgccagcc attcgacgta
    14641 atccatatca cgggtgatcg acggataacg gaagtcgata tccagcacat tcgccagttc
    14701 atcgaagtat tgtttatcaa tgcctttttc acgtaacaga ttgagatagc taaaaattgc
    14761 cgccacaacc tgatcgcgat tagccaggcc tttatcggtt aaagacgcag agatcgctaa
    14821 tacgccgctg ttgccgttga cgataggatc ggagttggcg ctaatgccct caactaatcc
    14881 ctgcttttgc agccagtcgg aaagtgtacc tgggctgcga ttaccaatca gataggtaat
    14941 caattcatcc gttttactac ggaacttcgc tgagttgtta tcgatgcgaa actcaacgcg
    15001 cagaacttta cgcggcattg ccgggacgta atgaatgata atgccctttt gcgcgtcggt
    15061 gactaccggc acggtgattt ccggtttttt gctctctttg ttcggcacgc gaccaaaggt
    15121 gtccgccgcc atttttgcca actccggcag cggtttattg ctgtaaataa ccgccttcat
    15181 cagattggcg gaatagtact tctcgtggaa atctttcagc gcctgctgca ccggattacc
    15241 aggtttatcg cttaaagttt cgaggttacc accagaaaac tttgaaccgg ggtgtgccgg
    15301 gttaatggtt tctgcgctga cctgtgccat gcgcatcccg tcacgcgtac gcgccatggt
    15361 taattcagcg ttcaccgcat tacgctcacg ttcggcatat ttcttgtcga gcaaaggttc
    15421 agcaatagca tcggccaggc ggtctaccgc accaggcaag gcgtcgttct caacttccag
    15481 atagaaagcc gtgcgatacg gcgcagtgct ggcattgtga ctaccgccgt gcattttgag
    15541 atattcggcc agactgtcag cctgcgggta ctttttcgac cccatcagac tcatatgttc
    15601 aaggtaatgt gccagcccct ggtacgcctc gggatcttcc agcgacccaa cgggcaccac
    15661 cagcgccgag agcgatttaa ctgcctgcgg atcagaaacc agcaagacca ccataccgtt
    15721 atccagacgt atagcctgat actggcggtt atctttatca cttttacgga tggtttcctg
    15781 aatcggctgc catcccgttt ctgcctgact taagggtgcc caaagggcaa ctaacaacaa
    15841 taatgctttg aaccaggtgc tgcggggcat tctcggacct cataatcaac ttaattttct
    15901 gtccagattc aacacgttaa cgcaaatcat ctgccagaat ttaatcttgt gctgcacgag
    15961 tcagcctatg tttatataac catcagtccg tgactggtgc gcatcataaa gtaagcggat
    16021 agattgcgca atttttatac agcactcatg actgattaaa gcgaaacagc ggtaacagga
    16081 aacgttgcga ctgttcaacg atggcctcca ttgtctctgg tgttaattgc cgccagagcc
    16141 tttgatacca gatatcatca ccttcgccac gcaccatcat gttaccttcg taagcctgaa
    16201 ggaatttcgt acgggctttt tgcaacgtgg aatcgtcatc cagcatggca tcgttttgcg
    16261 cgtcataaca ggtttttagc cacgcgccgc cactttcagg taacaccagc aatggcgcgg
    16321 acatcccttc acgatacccc tcaatcagtt gtgagaggta atgcaaagcc tgttcggctg
    16381 caagcggcgg aaaacgccac tcgccgtctt tgcgtagaaa aaggcgactt tcaccattac
    16441 caccgctggc acagtagaca aggtgttcca gccaaagttg cattccctgc gccacactta
    16501 ataaagaggg acgccagcgc aacaggccat ccggctgcac ctgcggcaac cagccagtta
    16561 tctgcacacc gttgcaggcg agatcaattt ccatactctg ccccggctgg cgacaggcaa
    16621 tgactctgtc ggcaagctgc tgcatctcct ggcactgagt ttcccagaaa atttcaccaa
    16681 aagcgccata cggtaaatcc cctgccgccc ggaagcgacg gaacaagcgt tcggcatcat
    16741 cctgctcaac cagtgcattc aataactgct gattgatttg ataacggcta agcccttcca
    16801 gaataaatgg ctcggtgtcg gggatttcgc tgtcttcagt acggaagttc acctgcaaac
    16861 gcatctggaa gaacgcccgc accggatgtg cccagaatcg ttgtagcgtt tccagcggca
    16921 cggtttccgg taaggtaaac ggcagcggct gaacaaattc agaatgtgct ttaccagcct
    16981 ggctggccgc aggtagccat tcacgagcat agctttgtcg ttcgcctggc tgatagtttt
    17041 gtggatcaaa cggcattcgg gtatggaggc aagtaagatg cgcttttacc cttgcctcgc
    17101 tttcatcaca gttgagcgct tcatcgcccg gtagataatg actttgcccg atgtagtcga
    17161 tcagttcctg caccagtacc gacgggaaac gttcactgtt atcctgaatg gaacgcccga
    17221 tatagctgat atagagtttt tgctgcgcgg aaattaacgc ttccaggaac agatagcggt
    17281 cgtcatcgcg acggctacgg tcgccacgct tcggtttctg gctcatcagg tcaaagccca
    17341 atggcgcaag ctgacgtgga taaacgccgt cgttcattcc cagcaggcaa accactttga
    17401 acggaattga acgcattggc atcagagtac aaatgttaac cggtccggcg agaaaacgct
    17461 ggctgatacg ttcttgatcc agacgctgtg ccagttcatc acgcaatagt gacagcggca
    17521 ccgcgtcgcc atactgcgca cctaaacctt cggcgataat cgcctgccat tgttgttcga
    17581 tcagcgtcat cgccgcttcg gtttccgcat ccggcaggaa gaaggcgctg agcatatcgc
    17641 gacaaaccgg caaccactct tctagcggac gctcctgtgc cagcccgcga cgccagatgt
    17701 ttagctgcat tagcagtgaa gccagatgcc ccaccagttc tgcaattaag ccgctcgatt
    17761 catcataagg tagaaccgat tgccactcgc cctgcgcgct ctccatcgcg tagcccagca
    17821 acatacgcgt caggccaaat cgccaggtgt gttgtccggt ggcggggagt tccagctcgc
    17881 gaacgttgtc gtcatctatc ccccaacgaa tgccggattc gttgacccac tggcgtaaat
    17941 aacgcagccc ttcttcggtg atgtcaaacc gcgccgccag caccggcaca tccagtaatg
    18001 ccagcacgtc ttccgacaca aagcggctgt ctggcaacga taacaggctg ataaacgctt
    18061 ccagtaccgg atgcgactgc cgcgcccgac ggtcggaaat ggcgtaaggc aggtaacgat
    18121 ccgcaggtgc gctaccaaac acagcccgaa taaacggact gtagctgtcg atatcagcca
    18181 ccatcacgat gatgtcgcgc ggagtaagtg tcgggtcttc ttccagcatc gccagcaggc
    18241 gatcgtgcaa aacttcaact tcacgctgcg ggctatggca aacgtggaag gtgatactgc
    18301 tatccagtgg atcaagcggg cgtttgttat cgctacggga aaactcttcg atgttcacac
    18361 cagcaacggc acggttttcc agttccagaa tgtcagactg aatgttatgc agcaggttat
    18421 ctggcgtgac atcgacaaag gcgtccagct cctggctgct ctccaggtca gaaaggagat
    18481 aaatgtagtc gcgcccaagc ttaccccatg aagccagcag cgggttgccg acatcctgtt
    18541 caccatcgct gttaaagagc tgcccggcat tttcgctatc acgaaatagc ggcaattcgc
    18601 gatcttcaaa actgtgtcga cgctgacggg tcagcagttt cgccagataa gcaggatctt
    18661 taatatcgcc ccagtaataa cggcaggggt tggtaaacag gagatggatt tcaatatgtt
    18721 tacccagcgc ctgtagcgcc tggagataaa caggcggtaa cgcggaaata ccgcatataa
    18781 agacgcgcga aggtaacccc ggcgggcagg tcgtcgcgga ctccagcgtt tcgataaagc
    18841 gctgatagag attggcgcgg tgccagcgcg gttgcccgag ttgatgggta tattccacca
    18901 gcgccttcca caacggggct tgccaggcct gtgcttctcc cagcccttca accaaatgtc
    18961 ctgtttccca ctgtgccagc cagtccggac gatagaccag atactggtca aacaggtccg
    19021 ccgcttttga ggaaagctgg aacagttttc gcttgtcgct atcgtcagtc agataatgcc
    19081 gcaacagggt aaagtcttcg cgctccagca attgcggcag cagagtcatc agtttccagc
    19141 tcatgctctg tttgttaaag gcgctctctt tggggatttc cggtaacacc cggacgaaca
    19201 tatcccagat aaagctcgct ggcagcggaa aatcaatgtt tgccgcaata ctaaactttt
    19261 gcgacagggt catttgcagc cactgcgcca taccggtact ttgcaccaga atcatctctg
    19321 gttcgaaagg atcgtccagc cgttcgcgtt cgacaataaa ctccatcaac gcttccagca
    19381 cgtccagacg attggaatgg tagaccctta acatagcggc tcctgactac tgacgattcg
    19441 ggcaatgcag gcgggtcatc tcgccttctc tgcccccggg tgaaactagc gtaacgctga
    19501 tgctgacaca tcccgcctgc gatgtctgca ttcggttgac ctgccagttg gcaggtggcg
    19561 aaatcgcgcg cagttgcgtt tgctgccaac catgccgcca gagctgttgg tactggtttc
    19621 tgctggcgag actgttcatt aatgttcgct ggatacccga taacgcagtg acaatcatca
    19681 ccatcagcac catcgcaacc attacctccg gcaggctaaa gccttgttga ttcttcaggg
    19741 aagctgacat aacgccctct ctttcagcgg acaaaaatcg ctccagccgc gtggcgaaaa
    19801 aacaatgttt ccatcaatga cttcgcctgt tcgccacaac gaaacgcctt cataaccggc
    19861 aaccaataag gcttcattat ctgccagtaa acgcaaacaa acccgggcat cggttccagc
    19921 gtactgcgag cactgaactg cgggctgcgt ctgccaggag tgcattttcc cccacgccag
    19981 cgccgactga acgatggcct ggcggcgcaa tgactgactt tctatgctca cgcgagaggc
    20041 aaaactgcga tcctgctgac tcattccttg caatagcaag ctacccaaaa ccagcagcat
    20101 caggaccaga gccagtgacg aaacaccttt ttcgcggttc acaggttgaa tcctgtcacg
    20161 ctatagctgg catccaccac ggtttgcggt tcagacttac tggcagcacg catattaacc
    20221 gtcaacaccg gcgagaagcc gctgacatcc tgacgtacga cctgaaaagt gtcgataatg
    20281 atggcatccg gattagtgac tttatcccag cccttacctt cacaggatgt cgcaccgcgt
    20341 agcgtttcca gcacatgctc cttcagacga aatccaatct ggtcagactc ttttaccggt
    20401 tcgcgatccc agataccgtt actgttcgca tcccactgca caatgataca gtcaccctgt
    20461 ccgacaattt ccagcccttc gccggtacag ctgccatgac aataacccgc cctctggaga
    20521 tgcttcgcga cggtaaatac ccgcagccag atttcatctt ccagcgccag cttacgggtg
    20581 ctcgttaaac tttcacgctg taacgcaggc agaaagcgtg cagcccccag caacaatacg
    20641 ctactgatcg ccatagcaat caacacttcc agcagagaaa aaccttgctc ttttacaggc
    20701 atccttctgt ttctccttgc tgacaaagcc ggagtcttcc ccacggcgaa accaccagcc
    20761 accactcgcc cgttgagttt ttgaagcgaa tatgcccggc ccatgcggta ttgcgcaggc
    20821 caaagaaagc aagcgaaggt gtcaggtcgc tcatttcgac ttcgggccag cgtggcacaa
    20881 agaccaatgg tgaactgcca tgacaggtat tggccccagc agcggaactc acaaggcacc
    20941 ataacgtccc ctccctgata acgctgatac tgtggtcgcg gttatgccag ttggcatctt
    21001 cacgtaaata gagcaaatag tcccgcgcct ggctggcggt ttgccatagc cgttgcgact
    21061 gctgccagta ttgccagcca tagagtccac ttgcgcttag catgaccaaa atcagcatcg
    21121 cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt catgccggca gtatggagcg
    21181 aggagaaaaa aagacgaggg ccagtttcta tttcttcggc gcatcttccg gactatttac
    21241 gccgttgcag gacgttgcaa aatttcggga aggcgtctcg aagaatttaa cggagggtaa
    21301 aaaaaaccga cgcacactgg cgtcggctct ggcaggatgt ttcgtaatta gatagccacc
    21361 ggcgctttaa tgcccggatg tggatcgtag ccttcaatct caaagtcttc gaaacggtag
    21421 tcgaagatgg attcgggttt acgtttgata atcaacttcg gcagcggacg cggttcgcgg
    21481 cttaattgca gatgagtttg atccatatga ttgctgtaca ggtgcgtatc gccaccggtc
    21541 cagacaaaat cacccacttc cagatcgcac tgctgcgcca tcatatgcac cagtaacgcg
    21601 tagctggcaa tgttgaacgg caggccgagg aagacgtcac aggagcgctg ataaagctgg
    21661 caagagagtt tgccgtctgc cacatagaac tggaagaatg catggcacgg tgccagcgcc
    21721 attttatcca gttcgcctac gttccacgct gaaacaataa tgcggcggga atccgggtcg
    21781 tttttcagct ggttcagtac cgtagtgatc tggtcaatat gacgaccatc tggcgttggc
    21841 caggcgcgcc actgtttacc atacactggt ccgaggtcgc cgttttcatc ggcccattcg
    21901 tcccagatgg tgacattgtt ttcgtgtaga taagcaatgt tagtgtcgcc ctgcagaaac
    21961 cacagcagtt cgtggatgat ggaacgcagg tggcaacgtt tagttgtcac cagcgggaat
    22021 ccatcctgca ggttaaaacg catctgatga ccaaaaatgg aaagcgttcc ggttccggta
    22081 cggtcgtttt tctgtgtgcc ttcgtcgagc actttttgca tcagttctaa atactgtttc
    22141 atggttcctc aggaaacgtg ttgctgtggg ctgcgacgat atgcccagac catcatgatc
    22201 acacccgcga caatcatcgg gatggaaaga atttgcccca tgctgatgta ctgcacccag
    22261 gcaccggtaa actgcgcgtc gggctggcgg aaaaactcaa caatgatgcg aaacgcgccg
    22321 tagccaatca ggaacaagcc agagacagct cccattgggc gcggtttacg aatatacagg
    22381 ttgaggataa taaacagcac cacaccttcc agcagcagct cgtaaagctg tgatgggtgg
    22441 cgcggcagca caccgtaagt gtcgaaaatg gattgccact gcgggttggt ttgcagcagc
    22501 aaaatatctt ctgtacggga gcctgggaac agcatggcaa acgggaagtt cgggtcaacg
    22561 cggccccaca attcaccgtt aataaagttg cccagacgcc cggcaccaag accaaacgga
    22621 atgagtggtg cgataaaatc agaaacctgg aagaaggaac gtttagtacg gcgggcaaag
    22681 ataatcatca ccacgataac gccaatcagg ccgccgtgga aagacatgcc gccgtcccag
    22741 acacggaaca gatacagcgg atcggccata aactgcggga aattgtagaa cagaacataa
    22801 ccaatacgtc ccccgaggaa gacgccgagg aagcccgcat agagtaagtt ttcaacttca
    22861 tttttggtcc agccgctgcc cggacgattc gcccgtcgtg ttgccagcca cattgcaaaa
    22921 atgaaaccca ccagatacat caggccgtac cagtgaagcg ccacgggtcc tattgagaaa
    22981 atgaccggat caaactccgg aaaatgcaga tagctactgg tcatctgtca ccacaagttc
    23041 ttgttatttc gctgaaagag aacagcgatt gaaatgcgcg ccgcaggttt caggcgctcc
    23101 aaaggtgcga ataatagcac aaggggacct ggctggttgc cggataccgt taaaagatat
    23161 gtatatgatc cgcgctataa ccctccgcga atcagcccgc ccatgccgcg acgctccata
    23221 aaggctgcaa cctgatggcg aacttcggtc gccagttgcg cttccagact acgctgcgca
    23281 agattttctg cttcggcata atcaatgcgc cgcagcaggt attttacccg cgctacagaa
    23341 cgtccgttca tagacagatg gcgataccca agcccaatga ggattgccac gcacatggga
    23401 tcgcccgcca tttcaccgca caaacggaga tcgattccat gtatttccgc ttcccgggcg
    23461 atcatcgcca gagctcgtaa cattgcagga tgaagactgt cataaatgtt cgccacccgg
    23521 gtattgttgc gatcaacggc cagaatgtat tgagtcagat cgttggtgcc aacagagatg
    23581 aaatcgaccc gctttgccag atgcggcagc ataaatacca ttgacggcac ttccagcatg
    23641 atgccgatac gtggtttggg aatttcgtaa ccgatcatct cctcgacttc acgtccggca
    23701 cgttcaatca ggcggcgtgc ttcatcaact tcatcgaggc ttgtgaccat cggcaacaga
    23761 atattcaggt tgcccgtagc ggcattagca cgcagcatcg cccgcacctg gatcaagaag
    23821 atctccggct gatcgagcgt aatgcgaatc ccacgccaac ccaggcatgg attctcttcg
    23881 ctgatcggca tgtaaggcag ctgcttatct gctccgacat ccagcgtacg caaggtgacg
    23941 ggtttatcat tgaacatttg cagcatcccc tgatactgcg ccacctgttc ttcttccgac
    24001 gggaaaccac tttgcagcat gaatgggatt tcagtgcgat aaagaccgat gccatcaata
    24061 cggctgccca gtttttcttc atgttccggg cttaaaccag cattgagcat gacttttata
    24121 cgctcaccgc tttttaactg cgcgggtaaa ttgacgtcat cttccgccag acggctaagc
    24181 tcaatctctt cactaattag ccgctgatat tcttgcagca gtaccggctc cggatcgacc
    24241 agcaattcac cgcgatagcc atcaacgatc agcgtcctac gatgcagcac cgaaggctga
    24301 atatccgcgc ccatcacggt agggatcccc agcgcacgta ccatgatcgc agcatgggag
    24361 ttggctgcgc catctcgcac gacaacaccg actaagcgat cctggggcag ctcagcaaga
    24421 gttgtcgctg acagttcatc tgccaccaga atgaaacgtt ccggccaggc gttcggccct
    24481 tgattagcgt catcaagatg aaacagcaat cgctgaccca gcgcacgtaa atcgccagcc
    24541 cgctctttga gatagttatc gcttagcgcg gcaaactgtt cggcaaattt ttcaatgacc
    24601 gtttttaccg cccactctgc caccgagcct ttatcaacct cggcaaacaa ttcgcgacgc
    24661 agccgggtgt ccgaaagtag gtgcgagtaa agatcgaaaa tagccgccgt ttctttttgt
    24721 gctccggcgg caaagcgttt gctgtagcgg cgaaactcgt ttgccgcttc ttccagcgcc
    24781 ccggtcagtc gttcgcgttc cagagccgga tccagcgttg atgcctgata cacctgttcc
    24841 attaaaggta acgtggcatc ctgccagcct tcggcaatcg ccacaccagg tgctgccggt
    24901 aatgcgcgga ttcgcgtctg gcgatattgc ccaaacaagg cagtcaactg cgactgagaa
    24961 agaatagctg ccatctgggt ggcaagcgtc accaggaagg attcttcact ttcgtcatac
    25021 tggcgcaact ctcgttgctg taccaccagt acaccaagca actggcgacg ttgaataatt
    25081 ggtacgccta aaaacgcgcg gaaacgttct tcttttacgg aggggatgta tttgaagctg
    25141 gggtgctttt gcgcatctgc aaggtttatc ggttccgcca gcctgccaac caggccgacg
    25201 atcccttcat caaacgcgag cgttacagtg cgaccgcgtg gttttttcag cccccgggtc
    25261 gccatcaggt agtaacaacg tcgatcatga tcggccaggt agaccgaaca gacctcggta
    25321 tccatcgcaa gacagatgtc ggtaaccaga atatttaacg cctcattcag gcgtggtgcg
    25381 ctggctacct tttcgactat ttcgcgcagg cgagtgagca taattggcgt gacttaacct
    25441 cttttacgtc gataagcaga tgtgttttgt ggttttggcg tattttcctg cagtgacatc
    25501 accacactcg cgaactcttt cattacccta cggtagacat cacgtttaaa tgacaccacc
    25561 tgtctgaccg gataccagta acttacccat cgccagccgt caaactctgg tgtactgctg
    25621 gtttgcatat tgatttctgc atcgccgctc accagctgca agagaaacca tttttgtttt
    25681 tggccgatac aaaccggctt cgtgtcccaa cgcaccaaac gtttcggtaa tttgtagcgc
    25741 aaccagttac gcgttgaagc aaggattcga acgtctttgc ggcttaatcc tacttcttca
    25801 aacaattcac ggtacatcgc ctgctctgcg gattctccgg ggttgattcc gccttgcgga
    25861 aattgccagg agtgctgacc aaatcgccgg gcccacatta cctgcccctg gcgattacaa
    25921 atcacgatac ctacgtttgg gcggtagcca tcgtcatcaa tcaccggact acctcaaaat
    25981 aaagctttat atacgaatga ttgtttcata ctccaagaag acggtaaacc actctctgca
    26041 gggcattaca cactaataac aattgaataa ctcacagtta tgtgcagagt tataaacaga
    26101 ggaaggggtg gatagccgtt tttattcacc ttttctgtgg atagagttgt gaagaactac
    26161 ggaattacta cgggaaaacc cggagcattc tgaataagcc cgatatagac attttaaaat
    26221 attcattatt agtcaatgca ttacatgtaa tttgagtaat gaaaaagagt aattcgtgac
    26281 ccaggtcaca cctctcattt acgggttggc gaaagatcaa ccaatgccgt atttatccac
    26341 agaatgtgcc actaagttaa gcactgaacc actaaaaact ggagtttcgt cgcacgtcaa
    26401 ggctgtaaat ggaaacagta gtggaggttt ttcacagtta tcccagcttt ctgtggataa
    26461 catggtgtaa gatcctgttt attttcagtg accagatttg gaaaacctat tgcagtgttg
    26521 cgcaactcga ttaccggcag cctaaaaagc aatataaatc agagaattga acaacacatg
    26581 tggaaaaagt tacactgcga atattcggca cataattgct gtttgttttt taaccaaggt
    26641 atcatgacat gtcccaacct cgcccactcc tctctcctcc cgaaactgaa gaacagttgt
    26701 tagcgcaagc acagcaactt tctggttata cattgggaga actggcggca cttgccgggc
    26761 tggttacgcc agagaattta aaacgcgata agggctggat tggcgtgtta ctggagatct
    26821 ggctaggtgc cagcgcaggg agtaaacctg agcaagattt tgctgctctg ggcgtggaac
    26881 ttaaaactat ccctgtggat agtcttggtc gtccgctgga aacaacattc gtttgtgttg
    26941 ccccgttaac gggtaatagc ggggtgacct gggaaaccag ccatgtgcgc cacaagctca
    27001 aacgcgtact gtggataccg gttgaaggtg agcgcagcat cccgctggcg cagcgtcgcg
    27061 taggatcacc gttgctgtgg agcccgaatg aagaggaaga ccgacagcta cgcgaagact
    27121 gggaagaatt aatggatatg attgttctcg gtcaggttga gcggatcacc gctcgtcacg
    27181 gggagtattt acagatacga ccgaaagcag cgaatgcgaa agcgcttacc gaagccattg
    27241 gtgcccgggg cgaacggatt ctgacgctgc cacgcggctt ttatttgaag aagaatttca
    27301 ccagtgcact actggcccgt cattttctga tccagtagcc atcgctttga cctgccgctt
    27361 tccgggcata taattaccgc ttcatttttt tggcagggct ttttagatgt tatttgcatg
    27421 gataaccgat cctaacgcct ggcttgcgct cggtacgctg acgctgctgg agatcgttct
    27481 tgggatcgac aatattattt tcctttctct ggtggtggca aagctcccca cagcacaacg
    27541 tgctcatgcg cgccgtctgg ggttggcggg agccatggtt atgcgtctgg cgctgctggc
    27601 atcaatcgcc tgggttacgc gcctgacaaa tccgcttttt acaatattca gtcaggaaat
    27661 ttccgcccgt gatttgattc tgcttctggg tggcttgttc cttatctgga aagccagcaa
    27721 ggaaatccac gaatccattg aaggtgaaga agaagggctg aaaacacgcg tttcatcatt
    27781 cctcggcgct atcgtgcaga ttatgctgct ggatattatc ttcagcctcg actcggtgat
    27841 taccgctgtg ggtctgtcag atcacctgtt tattatgatg gccgccgtgg tgattgccgt
    27901 aggcgtgatg atgttcgccg cgcgctcgat tggtgatttt gtcgaacgcc atccttcggt
    27961 aaaaatgctg gcgctctctt tcctgattct ggtcggcttt accctgattc tggaaagttt
    28021 cgacatccac gtaccgaaag gttacatcta cttcgcgatg ttcttctcta ttgcggttga
    28081 aagcctcaac ctgattcgca acaaaaagaa cccgctctga tactccgtac gctctcctgc
    28141 gggagggcgt actcactcct gttaccctca cagtttaaga ttttcctgct ttcaagatat
    28201 ataacgtcgg tttataaaca gactattatc ataggtgggc caaatgagga taaccggatg
    28261 aaaaaatggg cagtaataat ttctgcagtc ggactggcgt ttgctgtttc cgggtgttcc
    28321 agtgattacg tcatggcgac caaagatggc cgtatgattt tgaccgatgg aaaacctgaa
    28381 attgatgatg ataccgggct ggtgagttat cacgatcagc aaggtaacgc gatgcaaatt
    28441 aaccgtgatg atgtttcgca aattattgaa cgttaacaaa taaggtcagc atccggctgt
    28501 aatgccgatc agttaag
//
LOCUS       DAZPAC010000036        28102 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZPAC010000036 DAZPAC010000000
VERSION     DAZPAC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28102
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.036"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUPM_001261"
     CDS             complement(<1..215)
                     /locus_tag="ACHUPM_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0869095.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHUPM_001262"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHUPM_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR0869096.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHUPM_001263"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHUPM_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR0869097.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHUPM_001264"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHUPM_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR0869098.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHUPM_001265"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHUPM_001265"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR0869099.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHUPM_001266"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHUPM_001266"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR0869100.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHUPM_001267"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHUPM_001267"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR0869101.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="ACHUPM_001268"
     CDS             complement(4604..5326)
                     /locus_tag="ACHUPM_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0869102.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHUPM_001269"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHUPM_001269"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0869103.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="ACHUPM_001270"
     CDS             6526..6744
                     /locus_tag="ACHUPM_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR0869104.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHUPM_001271"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHUPM_001271"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0869105.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="ACHUPM_001272"
     CDS             complement(7478..7678)
                     /locus_tag="ACHUPM_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR0869106.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHUPM_001273"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHUPM_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFR0869107.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHUPM_001274"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHUPM_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR0869108.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="ACHUPM_001275"
     CDS             10175..12088
                     /locus_tag="ACHUPM_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0869109.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="ACHUPM_001276"
     CDS             12088..13110
                     /locus_tag="ACHUPM_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR0869110.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="ACHUPM_001277"
     CDS             13104..13322
                     /locus_tag="ACHUPM_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR0869111.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="ACHUPM_001278"
     CDS             13376..14245
                     /locus_tag="ACHUPM_001278"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR0869112.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="ACHUPM_001279"
     CDS             complement(14300..14704)
                     /locus_tag="ACHUPM_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR0869113.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="ACHUPM_001280"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="ACHUPM_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR0869114.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15689..17779
                     /locus_tag="ACHUPM_001281"
     CDS             15689..17779
                     /locus_tag="ACHUPM_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR0869115.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHUPM_001282"
                     /gene_synonym="dapC"
     CDS             complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHUPM_001282"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR0869116.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHUPM_001283"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHUPM_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR0869117.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="ACHUPM_001284"
     CDS             complement(19747..20349)
                     /locus_tag="ACHUPM_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR0869118.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="ACHUPM_001285"
     CDS             complement(20339..20506)
                     /locus_tag="ACHUPM_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR0869119.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHUPM_001286"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHUPM_001286"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR0869120.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHUPM_001287"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHUPM_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR0869121.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="ACHUPM_001288"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="ACHUPM_001288"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR0869122.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="ACHUPM_001289"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="ACHUPM_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR0869123.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="ACHUPM_001290"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="ACHUPM_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR0869124.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cysG"
                     /locus_tag="ACHUPM_001291"
     CDS             26714..28087
                     /gene="cysG"
                     /locus_tag="ACHUPM_001291"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR0869125.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6788 a         7558 c         7111 g         6645 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt caccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgtccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc ag
//
LOCUS       DAZPAC010000037        27789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZPAC010000037 DAZPAC010000000
VERSION     DAZPAC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.037"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(1..2601)
                     /gene="acrF"
                     /locus_tag="ACHUPM_001292"
                     /pseudo
     CDS             complement(1..2601)
                     /gene="acrF"
                     /locus_tag="ACHUPM_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="ACHUPM_001293"
     CDS             complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="ACHUPM_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFR0869126.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4169..4831
                     /gene="acrS"
                     /locus_tag="ACHUPM_001294"
     CDS             4169..4831
                     /gene="acrS"
                     /locus_tag="ACHUPM_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFR0869127.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(4834..5013)
                     /locus_tag="ACHUPM_001295"
     CDS             complement(4834..5013)
                     /locus_tag="ACHUPM_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR0869128.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(5097..5981)
                     /gene="yhdJ"
                     /locus_tag="ACHUPM_001296"
     CDS             complement(5097..5981)
                     /gene="yhdJ"
                     /locus_tag="ACHUPM_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0869129.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6067..6363)
                     /gene="fis"
                     /locus_tag="ACHUPM_001297"
     CDS             complement(6067..6363)
                     /gene="fis"
                     /locus_tag="ACHUPM_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR0869130.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(6389..7354)
                     /gene="dusB"
                     /locus_tag="ACHUPM_001298"
     CDS             complement(6389..7354)
                     /gene="dusB"
                     /locus_tag="ACHUPM_001298"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR0869131.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(7683..8564)
                     /gene="prmA"
                     /locus_tag="ACHUPM_001299"
     CDS             complement(7683..8564)
                     /gene="prmA"
                     /locus_tag="ACHUPM_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR0869132.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(8576..10027)
                     /gene="panF"
                     /locus_tag="ACHUPM_001300"
     CDS             complement(8576..10027)
                     /gene="panF"
                     /locus_tag="ACHUPM_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR0869133.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10017..10259)
                     /locus_tag="ACHUPM_001301"
     CDS             complement(10017..10259)
                     /locus_tag="ACHUPM_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR0869134.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(10368..11717)
                     /gene="accC"
                     /locus_tag="ACHUPM_001302"
     CDS             complement(10368..11717)
                     /gene="accC"
                     /locus_tag="ACHUPM_001302"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR0869135.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(11728..12198)
                     /gene="accB"
                     /locus_tag="ACHUPM_001303"
     CDS             complement(11728..12198)
                     /gene="accB"
                     /locus_tag="ACHUPM_001303"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR0869136.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13176..14150)
                     /locus_tag="ACHUPM_001304"
     CDS             complement(13176..14150)
                     /locus_tag="ACHUPM_001304"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR0869137.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            14302..16242
                     /gene="csrD"
                     /locus_tag="ACHUPM_001305"
     CDS             14302..16242
                     /gene="csrD"
                     /locus_tag="ACHUPM_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR0869138.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAAQLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            16547..17590
                     /gene="mreB"
                     /locus_tag="ACHUPM_001306"
     CDS             16547..17590
                     /gene="mreB"
                     /locus_tag="ACHUPM_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR0869139.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            17656..18759
                     /gene="mreC"
                     /locus_tag="ACHUPM_001307"
     CDS             17656..18759
                     /gene="mreC"
                     /locus_tag="ACHUPM_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR0869140.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            18759..19247
                     /gene="mreD"
                     /locus_tag="ACHUPM_001308"
     CDS             18759..19247
                     /gene="mreD"
                     /locus_tag="ACHUPM_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR0869141.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19256..19849
                     /gene="yhdE"
                     /locus_tag="ACHUPM_001309"
     CDS             19256..19849
                     /gene="yhdE"
                     /locus_tag="ACHUPM_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR0869142.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            19839..21308
                     /gene="rng"
                     /locus_tag="ACHUPM_001310"
     CDS             19839..21308
                     /gene="rng"
                     /locus_tag="ACHUPM_001310"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR0869143.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            21376..25176
                     /gene="yhdP"
                     /locus_tag="ACHUPM_001311"
     CDS             21376..25176
                     /gene="yhdP"
                     /locus_tag="ACHUPM_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR0869144.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            25332..26777
                     /gene="tldD"
                     /locus_tag="ACHUPM_001312"
     CDS             25332..26777
                     /gene="tldD"
                     /locus_tag="ACHUPM_001312"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR0869145.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(26905..27770)
                     /locus_tag="ACHUPM_001313"
                     /pseudo
     CDS             complement(26905..27770)
                     /locus_tag="ACHUPM_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
BASE COUNT         6730 a         7016 c         7298 g         6745 t
ORIGIN      
        1 tcctgatacg acatacctgt ccagtcataa ccaatgcccg caggtaattt tgacgcaagg
       61 ttttccatca atgccatggc atcgccggaa ctggttcctg gcgcggcttc cccctgaatc
      121 tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac ccaatgtgaa
      181 gtggtaaagg cagagaatgg caccatttcg ccgttggcgc tgcggacata aagtttatcg
      241 acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt tttcacgcga
      301 ccacggtcga tgaagtcgtt aacgtaagtc ccacccagcg ccgttgaaat ggtctgattg
      361 atgtcagaaa gtgaaacacc taatgcctgc gccttttcct ggtcaacttc cagtttaaac
      421 tgcgcggtgt cttccagacc attaggacgc acgctgacta agctggcagg atgttgcgcc
      481 gccatgccaa gcaactggtt acgggcctgg gtcagggcat cgtgacccag cccagcctga
      541 tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc tggcatattg
      601 aatggaatga caaaaccgtc gcggatcttg cccaattcca ttttggcacg atggattacc
      661 gcttccgcac tgttttcgtc accactacgc tcttcccacg gtttcagact gacgaaagcc
      721 ataccggcgt tttgtgcctg gccgctgaag ctaaagccgt taaccgtaaa gacactttct
      781 acgttcgctt tctcgttatt cagatagtaa tccgtcactt gatccaacac tttttgcgtc
      841 cgctcttgcg tcgcgccagc gggtaactga atcatggtca gaaagacacc ctgatcctct
      901 tcaggtaaga aggaagaagg aagacggaag acgtaaaaac aacaccacca ttcctgcaac
      961 aatcagcgca tagatcagta aatatcgtcc tgtggaaccg aggattttgc cgacgctgtt
     1021 ggtgtagtgg ttaacgctat gatcgaaggt ggtattaaac cagccgaaga aaccgccctt
     1081 attttcgtga tgctcagcag agacgggttt aagcagcgtt gcacataacg caggggtaag
     1141 aatcaatgcc accagaacag aaagcgccat tgccgaaacg atggtgatag agaactggcg
     1201 ataaattgcc ccagtagaac cgccgaagaa tgccatcgga ataaataccg ctgacagcac
     1261 catcgcgata cccaccagtg cgccctgaat ttgcgacatc gatttttccg tcgcttcttt
     1321 tggcgggagc ttatcctcca tcatcacgcg ctcgacgttc tccaccacca ctatcgcatc
     1381 atcgacgagc agcccgatgg caagcaccat cccgaacatc gttagtgtgt tgatggagta
     1441 accaaaagcg gcgaggatgg caaacgtccc taacaacacc acgggtaccg caatggtggg
     1501 gatcagcgtt gctcgcatat tctgcaagaa cagatacatc accaggaaca ccagcataat
     1561 ggcttcgaac agcgttttta ccacttcgtg aatagaaagc tggacgaatg gcgtggtgtc
     1621 ataagggtag agaaccttca ttccctgcgg gaagaatggc tgtaattccg ccagttttgc
     1681 cttaatggct ttcgcggtat tgagagcgtt cgcgccggtt gctagcttaa tccccaggcc
     1741 cgccgccggt tttccgttga tacgagcgat aacgttatag ttttcaccgc caagttcaac
     1801 tcgtgcgaca tctttcaggc gtaccaccga gccgtcactg tttacgcgca gggttacttt
     1861 gccgaattct tccggatttt ttaaccgcgt ctgagcaata atcgaggcgt tcaactgttg
     1921 ccctggtaac gctggcgttc cgcccaactg cccggcagcg atctgatcgt tctgtacctt
     1981 caactggtta atcacatcaa ccggtgtcag tttatatttg tttagcagat cggcgtccag
     2041 ccagatacgc atcgcatact gtgcgccgaa aagctgtacg tcaccgacgc cattcagacg
     2101 gctaagcgta tctttaacgt tagaggccac atagtccgag atatcgtcct gtgtggtgtc
     2161 tgggttatca gagacaaagc ccgccaccat caaatagctg ctactggact tttcaacact
     2221 gatcccctgc tgctgaacct cctgcggcag caacggcgtg gcgagctgga gtttgttctg
     2281 cacctgcact tgcgcgatat caggatcggt cccggactgg aaggtaaggg taattgtcac
     2341 gctaccggcg gaatcgctgg tggaggacat atacatcagg ttatcgatac cattcatatt
     2401 ctgttcgata acctgcgtca ccgtatcctg cacggtctgc gcatcagcac ctggatagtt
     2461 tgcctaaaca gaaaccgcag gcggtgcaat ggttggatac tgagcgacgg gcaattgtag
     2521 gatcgccagt gcgcccgcca tcatcagaat aatggccagc acccatgcaa atatcggtcg
     2581 tcgaataaaa aagtttgcca tgtcagatta ccttacttcg atgcagtatc tgcgggggta
     2641 tcggtagtgg ctttaacctg ctctcccgga cgcgcttttt gcaggccgct gacaatgact
     2701 tgatcgccag atttcagacc ttcgctaatc aaccatttat cgccaatcgc ctgactggca
     2761 acgacagggc gcgcttccac ctgactttta tcgttaacaa tcagcacggt tgcatcacca
     2821 cgcggtgtgc ggctaacgcc ttgttgcggg ataagaatgg cgtcaggttg gacgccttca
     2881 tcaatccgtg cacgcacaaa catacccggc aaaagcgtat gttgcgggtt agggaagaca
     2941 gcacgtaggg ttatggagcc ggtgcattca tcaacggtca catcagagaa ttgcagcgta
     3001 cctttcaggg gataggtttg accgttttcc atgaccaact ctacgttgct ggtggcgttt
     3061 tccttatgca aatttccttg ctctacggat tgcttcagcc tcataaaatc gttgctggat
     3121 tgggtcacat caacgtagat aggatcgagc tgctggacag tcgccagttc agtcgtttgc
     3181 ccattagtga caagagcgcc ttcggtcacg gtcgatttgc cgatacgtcc gctaattggc
     3241 gcagtcactt tggtataagc gagattgatg cgagcgcttt cgactgtggc ttttgcggca
     3301 atcacggcgg catcggcctg gcgagcatca gcaatggcct ggtcgtactc ctgctggctg
     3361 atgtatttcg tacccacgag cggaacgtaa cgttttaccg tcaaatgcgc gatggcggcg
     3421 gcggcttcac ttttcgccag ttcgcctttc gcgctgtcat aatttgcctg ataggtcgcg
     3481 ggatcgatct ggtacaggga ctggcctgct tgtacatcgc tgccttcagt gaaattacga
     3541 ttcagtacga tcccgctaac ctgtgggcga acttcggcta tacggtaagc attggtgcgg
     3601 cctggtaatt cagtcttaac ttctaacggg gccgttttca caatatgaac ggtaacctgc
     3661 ggttcaccga cgtgagcttt ctcttctccc ttatcgttac aaccgacgat taaagccgcg
     3721 gagatcagaa taaaggaggg caggaggaaa aacctggcat gtttcgtcat tactattcct
     3781 caaaaaaaca aaagcgcgtt atttacccag aaaggcaacg cgttaactcg cagaaagaaa
     3841 aatacagttt gctatcctac aaattatcat tcgtcgatgt aaggaatagt tatgaatact
     3901 ggcgtctcaa ggcacataaa cacaaaaaaa gattaatatt ctactgtttt attttgacgc
     3961 gggttgaaag aggcagaatt aaaacctcgt aaattgaaat atatattcat gtggcgaatg
     4021 tattttaggt aaataatata tattatttat ttgacgattt atagcaactc aatgttaccc
     4081 tctcgtataa atacacatta gatgatagat taaccttcgc tattttctca ccctgtgtcg
     4141 aatatattta tttcctgaat aattaatcat ggcaaaaaga accaaagccg aagctctgaa
     4201 gacccggcaa gaactgattg aaactgccat cgcccagttt gcgcaacatg gcgtaagcaa
     4261 gacgacgttc aacgacattg ccgatgccgc taacgttacg cgtggcgcta tctactggca
     4321 cttcgagaac aagacccaac tgtttaatga gatgtggttg caacagcctt cattgcggga
     4381 gttaatccag gaacacttga cggctggatt agagcatgac ccgtttcaac aattgcgtga
     4441 aaaattgatt gtcggcttgc aatatattgc caaaattccc cgccagcagg cgttgctgaa
     4501 aatcttatat cacaaatgtg aatttaatga tgggatgctg gccgagggag tgatacgcga
     4561 aaagatgggc tttaatccgc agactctccg cgaagtattg caggcgtgtc agcaacaagg
     4621 ttgtgtagca aataacctcg atttagatgt tgtgatgatt attattgatg gtgccttcag
     4681 cggaattgtt caaaactggt taatgaatat ggcgggttat gatctttata aacaagcacc
     4741 cgcgctggtc gataacgtat taagaatgtt catgccagat gaaaacataa cgaaattaat
     4801 tcatcaaaca aatgaattaa gtgtcatgta aattcacatt ctgtcctgaa aattcagttc
     4861 gtcacagtta accagtttta gtggataaac tgagttcata ttacggcatt tgtctgtttc
     4921 tgttgtcagc agttcaatcc actgcatcag taaagtatca aacagaaaaa cgaagacagc
     4981 gaaaacgacg agccaccaat acttgcgaat cattacctga gcctgtgcat gtttaaccaa
     5041 gtctggcagg aaatatacac gaaatgaata atctgaaaac caggcttaca cttcctttac
     5101 tttgcaatga gatcggggtc aacttcgctt aaccggcttc gttttgacag gttgccggtc
     5161 tttctttttt tcactttcgc cagttcttcc gcggagtaat gcgacgcgac atccagccgt
     5221 cgaagcccca ttttgatgta ctcgctgttg atctcaatac caatgaattt tcgtccgctg
     5281 gcgatggcta cggcaccggt agtaaagcta ccggcaaacg ggtcgagaac gatatcgcct
     5341 gggttggaag aggcgagaat aatgcgtttc agtaaggctt ccggtttttg cgtcgggtgg
     5401 ttctcatatt catccatcaa ataacgcacg cgcggaaaat cccagacgtt acccggtact
     5461 ttttgatgat tgtatggctg tggaggattt ttgcgataat cgatcaacgc gcgctgcgat
     5521 ccggttttgg cttctaccag aatagcatca ccgttgaatg tgtagttctt tgcgtctttc
     5581 accatcatca ggatgggttc gtacatggag ccgtagtgtt ttttcgcctg tactccagaa
     5641 ctgtcatatg accagacgat gcgacttttg atggtaaaaa gcttgcggca ctggagatcg
     5701 ataaagggca tgttttccgt actgttcata atgtacatgc tgccctgctt tttcagaacg
     5761 cggtggcact ctacaatcac ttcaaacagc cagtcgataa acagatcttc tttccaggct
     5821 tcgatcagac catcaaaatt tttaccgatg ttatacggtg ggtcggcaaa gatcagatcg
     5881 acgctttcag taggtagctt tttaagttcg gcaagggcat caccgtgaat aatggtctta
     5941 gcttcattac caaaccgggt cggttcacat cctgttctca tggtcactcc ctttgtgaca
     6001 cctataaaaa aggcgcttcc ccatgccgag tagcgccttt ttaatcaagc atttagctaa
     6061 cctgaattag ttcatgccgt attttttcaa ttttttacgc agcgtaccac ggttgatgcc
     6121 catcatcagc gcagcacggg tctggttacc acgggtgtat tgcatcacca tgtccaacag
     6181 gggctgttct acttcagcca gtaccagctc atagaggtca ttcacatcct gaccattcag
     6241 ttgagcaaaa tagttcttca gtgcctgttt aaccgagtca cgcaggggtt tttgggttac
     6301 ctgatcctga gagttaacgg tagaaacggt cagtacgtca gaatttacgc gttgttcgaa
     6361 catagttctg tcagctcttt atttctgttt acgcaaaatt ttcgaagtat gcctccaacg
     6421 cctccagctg ttcgctggca tcctcaatgg cgttgaatgt gcgccgaaac tggtcatttg
     6481 gagcgtgttc ctggagatac caggaaacgt gtttacgtgc aattcggtac ccttttgccg
     6541 gaccataaaa gtcatgcagt tcccgaacgt gcgcgcaaag caagcgctta acctctgcca
     6601 aaggcagcgg gggcagcaac tccccagtgt ccagataatg ctggatttcc cgaaagatcc
     6661 agggtcttcc ctgagctgcg cggcctatca tcagggcatc cgcccctgta tagtcgagca
     6721 cagctctggc tttaagcggg tcagtaatgt cgccattcgc gataaccgga atggaaactt
     6781 tctgcttaac tgcccgaata ctgtcgtact cagcttctcc attgaacaaa caggcgcgtg
     6841 tacggccatg aatggtcaga gcctgaatgc cacagtcttc agccagttgg gcaatctctt
     6901 cgcagttacg gtgttccggt gcccagccgg tgcgaatctt cagggtaaca ggaacgtcca
     6961 ctgcattgac gacctcggta aggatcgatt taacgacatc cgggtactgc aagagggctg
     7021 aacctgcgag cttgcgattc acttttttag ccgggcaacc catattgata tcaataatct
     7081 gggcaccgct ttccacgtta atacgtgctg catctgccat ttctttcgga tcgctaccag
     7141 caatttgcac ggtgcgaata ccgggttcat caatgtgcac catccgtaaa cgagatttgt
     7201 cgctttccca aacctgtggg ttagaagaca tcatctcgga tactgtcaat ccggctccca
     7261 tctcgtagca caacgtccga aaaggtctgt ctgtaatgcc agccatgggc gctgcgatca
     7321 ggcgatttct gagctgatat tgtccgatgc gcatgagtta agaaatgacc atactgtgac
     7381 tgcaaggcgg cgtatattac gcattttttg cacgagatga aaggccaaac tttgaccaat
     7441 cctctgagat ggatcaaaga attgcattta aaatgagcgt ggtgagataa ttactcataa
     7501 aaatcatcat attagaaaat agtgactaaa atttacactc aaagaaattt gagtaagttc
     7561 tcaatttttc tttatgaatg aaaattttgg cacgcaaatt tgcgtaaata accggcaaat
     7621 ttacgtgcct tttgtgagct tgctcgcact tcgccccgcg tcaccctacg gcgatgcgaa
     7681 ggttaattct tacgaccggt aatgcggcac cactcttctt tttccacgac cgggtccagt
     7741 gcgaagctgt cgacataagc ttcacaaacg ctctctgcct ggcttgccag aataccggaa
     7801 aggcccagca aaccgcctga aaccggcagg acgctgatta acggtgccag ttcacgtaat
     7861 gggcctgcaa ggatgttagc gaccaccacg tcggctttca tttcttctgg ctgatctttc
     7921 ggtaagtaga gttccagacg gtcagaaacg ccattacgtt cggcgttgtc gcggctagcc
     7981 tgaatcgcct gcggatcgat atcaatacca atggcttttg ctgcacccag tttcagcgcc
     8041 gcgatagcca gaatgccgga accacagcca aagtcgatga ctattttacc ggttaaatcg
     8101 aggctgtcga gccactgcag gcacagagag gtagttggat gggtacccgt accaaacgcc
     8161 agccctggat ctaacatcac gttgacggcg ttttcgtccg gcacatcacg ccagctaggg
     8221 cagatccaca gtcgttcacc aaagcgcatc gggtggaaat tatccatcca ttcgcgctcc
     8281 cagtctttat cttccagttg ttcgatttta tgcgcgaagc ctgcgccgag cagcggatgg
     8341 ttttccagaa tcgccaccac gtcgttcata tcggtttcag cgtcgaacag accaatcaca
     8401 tcggtgtcgc cccacaggcg cgtttcgccc ggcagcggct caaatactgg cgtatcgtgg
     8461 gtatcctgaa aagtgataga aacggcaccc gcttccatca gcgcatcgct aagatcttca
     8521 gcgttcgcgc cggtggtgtt cagtttcagt tggatccaag gcatggcaaa actctttatt
     8581 tatcagtagt caaaacggta gcttgcggga cggatgtacc gaaacggttt ccgaccagga
     8641 aagccagcaa acttagtagt aacgagggca cgatagggtg gaagcccagg tactgaatat
     8701 tcagcgtcgc gagtacggca tacagcacgc cgccaacgat catcgcactt agcgcgcctt
     8761 tggcgttggc gcgttcccag taaagaccca gcaccagcgg ccacaggaaa acggcttcca
     8821 gcccaccgaa ggccagcaaa ttcagccaga tgatcatttc tggcggcttc caggcggcaa
     8881 gcagcagcaa cgcgccgaga actaacgtaa ttaccgccga catccgcttc agacgcgtct
     8941 cgttttgcat ttgatccgga cggatattca gatagagatc tttaatgatc gtagcggaac
     9001 tttgtagcaa ttgggcgtta attgtcgaca tgatcgcagc cataggtgca gccagaaaga
     9061 tcccggcagc aaacggtggc agcactttta ccattaacgt cgggatcacc agatccggta
     9121 cggtgagatc ggggatcact gcacgaccta acgctccggc taggtgcata ccgaacatca
     9181 ggattgcgac tacaatcgta ccgatgatga tcccccggtg taccgctttg ctgtctttat
     9241 aagagataca gcgcaccgca gtatgcggca ggccaatcac gccaaagcac accagtaccc
     9301 agaacgacgt cataaaggca ggcgacagaa tatcgtcagc gccttgtggc gtaaccagtt
     9361 gcggatcgat ggtttgcaag gtctgtactg cgttgcttaa gccgccagcg gcatgtacca
     9421 cgccaataag cagcacaacg gtgccaatca gcatcacaag cccttgcatg gtgtcgttca
     9481 gcacgctggc gcgaaagccg ccaaaggcgg tatacaacgc aatgctgata ccgaaaatca
     9541 gcagcccggt ttcataagga atacccgccg cggtttccag caggcgcgca ccgccgataa
     9601 actgcacggt cattgcccca acgaacgcaa ccagcaaact caaactcgcc agccacacca
     9661 gaagacgact ctggtagcgg gcaaacagca tgtcgttcag cgtcactgca ttgtagcggc
     9721 gcgcaagaat cgcaaacttc ttgccgagaa taccgagtga aagccagact gcaggaagct
     9781 gaatcatcgc cagcaatacc cagcccagcc cgtatttata agcagctcct ggcccgccga
     9841 taaacgaact ggcactgata taggtcgcgg tgagcgtcat cgccagcaca ataccgccca
     9901 tagagcggct gccgaggaaa tactcattaa ggaaggtgcc ggtgctccgt ttacgcatcg
     9961 cataaaccga gataccgaac accaccacca gataggcgac cagcggtaga attacttcaa
    10021 gctgcatcgt catcctccag tgggatatcg cgatagataa atttcaccat cgcccagcac
    10081 agtccaataa acagtagcgg cgtcaggatg caggccatct caaaccagcg cggaaagccg
    10141 gtaaaaccgg gggcaacgcc aggtaagtaa gcggctacta accaaactgc cagatacaaa
    10201 agggtcagcc ccagcgccca gcgcgcctct ttatgggcct gaacaaaacg agtgtccatt
    10261 ttttgtccct gatgggtgaa gaaagcgagg attgtacctt atgggggttg tcgatcccca
    10321 gtaataaaaa aggccggaaa atccggcctt ttggcgcttt tgcagtctta tttttcctga
    10381 agaccgagtt ttttctccag atagtggatg ttagtgccac catgctggaa gttctcgtca
    10441 ttcatgatgc ggatctgcag atcaacgttg gttttgatac cgtcgatgat cagctcctgc
    10501 agcgcattct tcatgcgggc aatcgccacg tcacggtttt caccgtagca aatcagctta
    10561 ccgatcattg agtcatagta cggcggtacg gtgtagcccg cgtagatatg agactcccaa
    10621 cgtacgccaa aaccgccagg tgcgtggaaa cgggtgattt tgcctgggct tggcaggaag
    10681 gtgttcggat cttcggcgtt gatacgacat tccaccgcat ggccgcgaac gtgaacttct
    10741 tcttgcttga tcgacagcgg ttgaccggca gcgatacgga gctgttcttt gatcaggtca
    10801 acgccagtga tcatttcggt aaccgggtgt tctacctgaa tacgggtgtt catttcgatg
    10861 aaatagaact cgccgttttc gaacagaaac tcgaaagtac ctgcaccgcg atagccgata
    10921 tcaacacacg ctttagcgca acgttcgccg atgtagcgac gcagttccgg ggtaatgccc
    10981 ggtgctggcg cttcttcgac cactttctgg tggcggcgct gcatggagca gtcacgttcc
    11041 gccagataga tagcgttgcc ccgaccgtca gccagtacct gaatctcgac gtggcgagga
    11101 ttttccaggt atttctccat gtaaaccata tcgttgctga aagcagcttt cgcttccgca
    11161 cgggtcatgg agatggattg tgccagttca gcgtcgccgc gcactacgcg cataccgcga
    11221 ccaccgccgc cgccggaggc tttgataatc accggataac caatgcgttt agcaatggca
    11281 cggtttttat ccatatcgtc gcccaacggg ccgtcagaac ccggtacgca agggacgccc
    11341 gcttttttca tcgccgcgat tgcggatact ttgtcgccca tcaggcgaat ggtttctgct
    11401 ttcgggccga tgaagataaa gccggagcgt tcaacctgct cggcaaagtt ggcgttctcg
    11461 gagaggaagc cgtaacccgg atggattgct actgcgccgg tgatttcagc ggcgctgatg
    11521 attgccggga tgttcagata actttttact gacggagcag ggccaataca gaccgtttca
    11581 tctgccagta atacgtgttt tagatcgcga tccgcgctgg agtgcacagc gacagtcttg
    11641 atgcccagtt ctttacaggc acgaagaata cgcaatgcaa tctcgccgcg gttggcaata
    11701 acaattttat ccagcatgtt cgcctcgtta ctcgatgacg accagcggct cgtcaaattc
    11761 taccggttgt ccactttcga ccagaattgc tttcacggta ccggatttgt ccgcttcgat
    11821 ctggttcatc attttcatgg cttcaacgat gcacagggta tcgcccacgt tgactttctg
    11881 acccacttcg atgaacgctt ttgcgtccgg gcttggggtg cggtagaaag taccaaccat
    11941 cggggaacgt acgatgtgac cactgatttc cgctgctgct ggcgcttcca tggaaggaac
    12001 ggtcgccgga gcggctgcgt tagattgagc tggctgctgc atcattggtg cagcgtaagc
    12061 ttgttgcatc acagggaaac ttgcggcagg agctgcacgg ctaatgcgta ctgactcttc
    12121 gccttcagaa atttccagtt cggagatgcc tgattcttca accagctcga tcagtttttt
    12181 aatcttacga atatccatga gtgggttccg tactctttgt ttagtgtgat tgtgacaggc
    12241 gtttcaccgc cgtctgtaaa gcgtatgccc cgacagctgt atgcatagcg ataaattcca
    12301 gcaggccggg acgccggtct attttgcccc agagtatcaa tgttagactt gacggacatt
    12361 gtgcagcgat ctgctgctat cctccggcaa aaaacaaaat ataccttcgt tgcgccaatg
    12421 tcaccttttc cgcagcgcaa aaactgcgtc agggaggacg aggtcgcaca ttataacgat
    12481 ttcgtagcaa ttggcagcta aatactggtc tcatcaggga agataatcaa cagctaacat
    12541 gtaaataacc ttcaacaccg tgtaatttgc aacaagccgc acaattcacg aaattagcgc
    12601 catcatcgac ggaacttctt ataacgtaag gtgaaaagct gtaaagccag ccctgcgaag
    12661 atgaggggct gcaatgagat aatcttcaca gaccacaaat aatgtaggga tgccaggatc
    12721 gcgacaagat agacgacatt acgcagcggt tgctggggat tacccaattt tcactgcatc
    12781 gtctggatag atgtgaatgc taaagcacgc aaaatcaacc gactgacaat tctcagcatt
    12841 aaataggttc gagtggttaa tttgtagtca aataaaatca gattattcac cccatggtac
    12901 cagaaatcag taacaggtta agcgtagtgt cgcccaggca aagcaccata aacctaacag
    12961 gcgacggagg cgtatcaata atggctgttt agaggagtat caatgcgcat caatttgtgg
    13021 tcagtcgaaa tcatgacttt ccagtttgga tgatgttgta caacttcctg accaaggtgt
    13081 tcgacgaaag ctgtccggct aaccgctttt cccgttaacc gattgaataa tgatgtcaaa
    13141 atgagttccc tctcttatta ttccttgcta aatggttagt taaccttcac cagcgtgcga
    13201 ccctggatct ggttattaat gatggcctcg gcaaagttcg gtgcctctga cagagatatc
    13261 tcttttgccg cctgggtata gaatgattcc ggtaaatcgg cgaccagtcg ctgccaggct
    13321 tgtgcgcggc gttctggtgg cgtcattact gaatccaccc cttgcaaacg gacattacgc
    13381 agaataaatg gcatgaccgt ggttggcaga gtaaaaccac ccgccagacc acaggccgcc
    13441 acgcagccgc cgtaattcat ttgcgccagc acttttgcca gcactttgtc gccaacagtg
    13501 tcaattgccc cagcccagac ttgtttttcc agaggacggg attcggcaaa ctcatcacga
    13561 gggagaatac ggctggcacc taaacttttc agatattcat gggtactttc gcgaccggaa
    13621 acggcaacga cctgataacc caacttatgc agcagcgcca cggcggtact gccgacgcca
    13681 ccactggcac ccgtcacgac aatctccccg tcctgcgggc gaacaccggc atcttccagc
    13741 gccatcacac acagcatggc ggtaaaaccg gcagtaccga taatcattgc tttacgcgcg
    13801 tccagccctt gcggcatggc aaccagccag tcacctttca ctcgcgcctg ctccgccagc
    13861 ccaccccagt ggttttcacc aacgccccag ccagtgagta acacctcctg accggcatga
    13921 aaacgcggat cttcgctggt gcgtacagtt ccggcaaaat cgatcccagg aatcatcgga
    13981 aaattacgga tgatttttcc cttaccggta atcgccagcg catctttata gttcaggctc
    14041 gaccagtgaa catcgaccgt gacatcgccc tccggcaggc gactttcgtc cagagtctgt
    14101 actgatgcga gagttttgcc gtcctgctgt tctaaaagta acgcctgcat aagtggtcct
    14161 catgtgcatg atggattgga aaataatttc tgaagattat actcgctaat ggaaaagcaa
    14221 agccgatgaa gcgcaataaa ttgcgagata aatctgattt gctagtatgc ccgcttcctc
    14281 actatcggag ttaacacaag gatgagatta acgacgaaat tttcggcctt tgttacgctg
    14341 ctcaccgggt taacaatttt tgtgactttg ctgggctgtt cgctaagttt ctacaacgca
    14401 attcagtata agtttagtca tcgcgttcag gcggtggcga cggcgattga tacccacctt
    14461 gtgtcgaatg acttcagcac attaaggcca caaattaccg aattaatgat gtcggcagat
    14521 atcgttcgtg tagacctgct ccatggtgat aaacaggttt ataccctggc cagaaatggt
    14581 agttatcgtc cggttggctc cagcgatctg tttcgcgaac tgagtgttcc gttgataaag
    14641 catccgggga tgtcgctgcg tctggtttat caggatccga tgggcaacta tttccattct
    14701 ttaatgacta ccgcgccgct cacgggggcg attggcttta tcattgttat gctcttcctg
    14761 gcggtacgct ggttacaacg gcaacttgcc gggcaagaat tgctggaaac ccgggctact
    14821 cgtatcttaa acggtgagcg tggctctaat gtgttgggaa ccatctatga atggccgccc
    14881 agaaccagca gtgcgctaga tacgctgctt cgtgaaattc agaacgcacg cgaacaacac
    14941 agccgtcttg atacgctgat ccgctcttat gccgcccagg acgtgaaaac cggcctcaat
    15001 aaccgactct ttttcgataa tcagttagca acgttactgg aagatcagga gaaagtaggt
    15061 acccacggga tcgtgatgat gattcgtctg ccggatttca atatgttgag cgacacttgg
    15121 gggcacagcc aggttgaaga acagttcttc actctgacta atctgctgtc gacatttatg
    15181 atgcgctacc ctggcgcact gctggcgcgt taccaccgca gtgattttgc tgcgcagtta
    15241 ccgcaccgga cgttaaaaga ggcagagagc atcgccggtc agttaatcaa agccgtcgat
    15301 accttgccga acaataaaat gctcgatcgc gacgatatga tccacattgg tatctgcgcc
    15361 tggcgtagtg gtcaggatac cgagcaggta atggaacatg cagagtctgc cacgcgtaat
    15421 gcgggattgc agggcggcaa tagctgggct atttacgatg actcgttgcc tgaaaaagga
    15481 cgcggtaatg ttcgctggcg tacgcttatc gagcaaatgc tcagtcgcgg cggcccgcgc
    15541 ctttatcaaa aaccggcggt tactcgcgaa ggtcaggttc atcatcgcga actcatgtgc
    15601 cgcatcttcg atggtaatga agaggttagc tcggcggagt atatgccgat ggtcttgcag
    15661 tttggcttat cggaagagta tgaccgtctg caaatcagcc gtcttattcc actattgcgt
    15721 tactggccag aggaaaatct ggcgattcag gttaccgttg agtcgctgat tcgcccgcgt
    15781 tttcagcgtt ggctgcgcga tacgttaatg caatgtgaaa aatcacaacg aaaacgcata
    15841 attattgaac ttgcagaggc cgatgtaggt caacatatca gtcgtttaca acctgttatt
    15901 cgtttagtga atgctttagg ggtacgggta gccgtcaacc aggctggttt gacgctggta
    15961 agcaccagtt ggatcaaaga acttaatgtt gagttactca agctccatcc ggggctggtc
    16021 agaaacattg agaagcgaac ggagaaccag ctgctggttc aaagcctggt ggaagcctgc
    16081 tccgggacca gcacccacgt ttacgccacc ggcgtgcgtt cgcgaagcga gtggcagacc
    16141 ctgattcagc gcggtgttac aggcgggcaa ggggattttt tcgcgtcctc acagccactt
    16201 gatactaacg tgaaaaaata ttcacaaaga tactcggttt aacctgccgt ttaatccgtt
    16261 ttcacgtaga ataacgcgcg ctgcgtctca tgggagtgtg cttgtctgct cgccagattg
    16321 ttgcagcaca tatgcagatg aatgacctta cgcggttgca aacaggcgag gaatgctgct
    16381 gatgcattaa gcctttctgg actcaggcag agatttgtaa caaaggaaac gaactgcact
    16441 aattttcacc gtagcagatg atttttgcgc cttgtcgctg ctgcgtgtgg ttggtaaagt
    16501 aagcggattt tcttttccgc cccagctttc aggattatcc cttagtatgt tgaaaaaatt
    16561 tcgtggcatg ttttccaatg acttgtccat tgacctgggt actgcgaata ccctcattta
    16621 tgtaaaagga caaggcatcg tattgaatga gccttccgtg gtggccattc gtcaggatcg
    16681 tgccggttca ccgaaaagcg tagctgcagt aggtcatgac gcgaagcaga tgctgggccg
    16741 tacgccgggc aatattgctg ccattcgccc aatgaaagac ggcgttatcg ccgacttctt
    16801 cgtgactgaa aaaatgctcc agcacttcat caaacaagtg cacagcaaca gctttatgcg
    16861 tccaagcccg cgcgttctgg tttgtgtacc ggttggcgcg acccaggttg aacgccgcgc
    16921 aattcgtgaa tccgcgcagg gcgctggtgc ccgtgaagtc ttcctgattg aagaaccgat
    16981 ggctgccgca attggtgctg gcctgccggt ttctgaagcg accggttcta tggtggttga
    17041 tatcggtggt ggtaccactg aagttgctgt tatctccttg aacggcgtgg tttactcctc
    17101 ttccgtgcgc attggtggtg atcgtttcga cgaagctatc atcaactatg tgcgtcgtaa
    17161 ttacggttct ctgatcggtg aagccaccgc agaacgtatc aagcacgaaa tcggttcggc
    17221 ttacccgggc gatgaagtcc gtgaaatcga agttcgtggc cgtaacctgg cagaaggtgt
    17281 tccacgcggt tttaccctga actccaacga aatcctcgaa gcactgcagg aaccgctgac
    17341 cggtattgtg agcgcggtaa tggttgcact ggaacagtgc ccgccggaac tggcttccga
    17401 catctccgag cgcggcatgg tgctcaccgg tggtggcgca ctgctgcgta accttgaccg
    17461 tttgttaatg gaagaaaccg gcattccagt agttgttgct gaagacccgc tgacctgtgt
    17521 ggcgcgcggt ggcggcaaag cgctggaaat gatcgacatg cacggcggcg acctgttcag
    17581 cgaagagtaa tcggatgcag gcaggggaaa tgtctgttta ccctgcctgg tctgatacga
    17641 gaatacgcat aacttatgaa gccaattttt agccgtggcc cgtcgctaca gattcgcctt
    17701 attctggcgg tgctggtggc gctcggcatt attattgccg acagccgcct ggggacgttc
    17761 agtcaaatcc gtacttatat ggataccgcc gtcagtcctt tctactttgt ttccaatgct
    17821 cctcgtgaat tgctggatgg cgtatcgcag acgctggcct cgcgtgacca actagaactt
    17881 gaaaaccggg cgttacgtca ggaactgttg ctgaaaaaca gtgaactgct gatgcttgga
    17941 caatacaaac aggagaacgc gcgtctgcgc gagctgctgg gttccccgct gcgtcaggat
    18001 gagcagaaaa tggtgactca ggttatctcc acggttaacg atccttatag cgatcaagtt
    18061 gttatcgata aaggtagcgt taatggcgtt tatgaaggcc agccggtcat cagcgacaaa
    18121 ggtgttgttg gtcaggtggt ggccgtcgct aaactgacca gtcgcgtgct gctgatttgt
    18181 gatgcgaccc acgcgctgcc aatccaggtg ctgcgcaacg atatccgcgt aattgcagcc
    18241 ggtaacggtt gtacggatga tttgcagctt gagcatctgc cagcgaatac ggatattcgt
    18301 gttggtgatg tgctggtgac ctccggtctg ggcggtcgtt tcccggaagg ctatccggtc
    18361 gcggttgtct cttccgtaaa actcgatacc cagcgcgctt atactgtgat tcaggcgcgt
    18421 ccgactgcag gactgcaacg tttgcgttat ctgctgctgc tgtggggcgc ggatcgtaac
    18481 ggcgctaacc cgatgacgcc ggaagaggtg catcgtgttg ctaatgaacg tctgatgcag
    18541 atgatgccgc aggtattgcc ttcgccagac gcgatggggc caaagttacc tgaaccggca
    18601 acggggatcg ctcagccgac tccgcagcaa ccgacgacag gaaatgcagc tactgcgcct
    18661 gctgcgccca cacagcctgc tgctaatcgc tctccacaaa gggctacgcc gccgcaaagt
    18721 ggtgctcaac cgcctgcgcg tgcgccggga gggcaatagt ggcgagctat cgtagccagg
    18781 gacgttgggt aatctggctc tctttcctca ttgcgctgtt gctgcaaatc atgccctggc
    18841 cggataacct gattgttttc cggccaaact gggtgttact catcttgttg tattggatcc
    18901 tggccttgcc tcatcgcgta aatgtgggca caggttttgt gatgggtgcc atactggatc
    18961 tgatcagcgg ctcgacgctt ggcgtacgcg tattggcgat gagcatcatt gcttacctgg
    19021 tggcgctgaa ataccagctt ttccgcaacc tcgcattatg gcagcaggcg ctggtcgtca
    19081 tgttgctttc gctggtggtg gatattattg ttttctgggc agagttttta gtgattaacg
    19141 tctctttcag accggaagtg ttctggagta gtgtagtcaa tggggtgctc tggccgtgga
    19201 ttttcttgct gatgcgtaaa gtccgtcagc agtttgcagt gcaataaagg tttctatgac
    19261 ttctctgtat ttagcttccg gttctccgcg tcgtcaggag ttacttgcgc aacttggtgt
    19321 gacctttgaa cgtattgtta cgggcattga ggagcagcgt cagccgcagg agagcgcgca
    19381 gcagtatgtt gtgcgtctgg cgcgcgagaa agcacaggcg ggtgtcgcgc aaacggcgca
    19441 ggatctcccg gtgctgggtg cggatactat cgttatcctg aatggagaag tgctggagaa
    19501 accgcgcgac gcagagcatg cggcgcagat gttgcgcaaa ttatcgggtc agacccatca
    19561 ggtgatgacg gcagtggcgt tggccgacag ccagcacatt ctcgattgcc tggtggtcac
    19621 cgatgtgact ttcagaacgt taacagacga agacatcgcg ggctatgtcg ccagcggtga
    19681 accgttagat aaagcaggtg catacggtat tcaggggctg ggtggctgtt ttgtcaggaa
    19741 gataaatggc agctatcacg ccgtagtcgg cttacctctg gttgaaacgt atgaattatt
    19801 aagtaatttt aacgcactgc gtgagaaaag ggataaacat gacggctgaa ttgttagtaa
    19861 acgtaacgcc ttcggaaacg cgagtggcgt atattgatgg cggtattctg caggaaattc
    19921 atattgaacg tgaggcgcga cgcggaatag taggcaatat ctacaagggt cgtgtaagtc
    19981 gtgtacttcc gggtatgcag gcggcttttg tagatattgg gctggataaa gccgcgtttc
    20041 ttcatgcatc cgatatcatg ccgcacaccg aatgtgtggc gggtgaagaa caaaagcaat
    20101 tcacggtgcg cgacatctcg gaactggttc gtcaggggca agatctgatg gtgcaggtgg
    20161 tgaaagatcc gcttggcact aaaggtgcgc gcctgaccac cgatatcacg ctgccttctc
    20221 gctatctggt ctttatgcca ggggcttctc acgttggggt ttcccaacgt attgaaagcg
    20281 aatcagaacg tgaacgcctg aaaaaagtgg tcgcagagta ttgcgacgag cagggcgggt
    20341 ttatcatccg taccgcagcg gaaggggttg gcgaggctga actggcctcc gatgccgctt
    20401 atctgaaacg cgtctggacc aaagttatgg agcgtaaaaa gcgcccgcag acccgttatc
    20461 agctgtacgg cgaactggcg ctggctcagc gtgttctgcg tgatttcgcc gacgccgaac
    20521 tggaccgcat tcgcgttgac tcacgcctga cttacgaagc attacttgag ttcacctcgg
    20581 agtacattcc cgagatgacc agcaagctgg agcattacac cggacgccag ccgattttcg
    20641 atctctttga tgtcgaaaac gaaatccagc gagcgctgga acgcaaagta gaactgaaat
    20701 ccggtggtta tctcattatc gaccagaccg aagcgatgac caccgtggac atcaataccg
    20761 gagcgtttgt cggtcatcgc aatctggacg acaccatttt caataccaat attgaagcga
    20821 cgcaggctat cgctcgccag ttacggttgc gtaatctggg cgggattatc attattgatt
    20881 tcatcgatat gaataatgaa gatcaccgcc gccgcgtgct gcactcgctg gagcaggcgt
    20941 tgagcaaaga ccgggtgaaa accagcgtta atggtttttc ggcgctgggg ctggtggaga
    21001 tgacgcgtaa acgcacccgc gaaagcattg agcacgtact gtgtaacgaa tgcccaacct
    21061 gccacggtcg cggaacggtg aaaaccgtgg aaacggtatg ctatgaaatc atgcgcgaga
    21121 ttgttcgtgt ccaccatgct tacgactccg accgtttcct ggtctatgct tctccggcag
    21181 tagccgaagc cttgaaaggc gaagagtcac actcgctggc ggaagtggaa attttcgttg
    21241 gcaaacaggt taaagtacaa attgaaccgc tctataacca ggagcagttt gacgtcgtaa
    21301 tgatgtaaac agatgctggg ccgccatccg gcaaagggtt tttgagtcac atttttagca
    21361 gacaaggagt gacgggtgag gcgattgccg gggattttac tgcttactgg agccgcgctc
    21421 gttgtgatcg ctgccctgct ggttagcggc ctgcgtattg ctttaccgca tcttgacgcc
    21481 tggcgtccgg aaatcctcaa caaaatagaa tccgcgactg gcatgccggt agaagccagt
    21541 cagctctcag ccagctggca gaattttggc ccgacgcttg aagcacacga catccgtgca
    21601 gaactaaaag atggcggcga attttcggtt aaacgcgtta ctctggcgct ggatgtctgg
    21661 cagagcctgt tacatatgcg ctggcagttt cgcgacctca ctttctggca gctgcgcttt
    21721 cgcaccaaca ctcctatcac cagcggtggt agcgatgaca gtctggaagc cagtcacatc
    21781 agcgatctgt ttcttcgtca atttgaccat ttcgatctcc gcgacagtga agtcagtttc
    21841 ctgacgccat ccggtcagcg tgccgagctg gcgatcccac aactcacctg gctgaacgat
    21901 ccacgtcgac accgtgcgga aggcctggta agcctctcca gccttaccgg acagcacggc
    21961 gtgatgcagg tgcgcatgga tttgcgcgat gatgaggggt tgttaagcaa tggtcgcgtc
    22021 tggctacagg cggatgacat cgacctgaag ccgtggctcg gtaaatggat gcaggacaat
    22081 attgctctgg aaacggcaca gttctccctt gaaggctgga tgacgatcga caaaggcgat
    22141 gtaaccggcg gtgacgtctg gctgaaacag ggcggtgcca gctggttggg cgagaagcaa
    22201 acgcatacgc tgtcggtgga taatctgacc gcgcatatta cgcgtgaaaa tccgggctgg
    22261 cagttctcta ttcccgatac acggatcacg atggacggca aaccctggcc gagcggagca
    22321 ttgacgctgg cctggatacc ggaacaggac gttggcggca aagacaataa acgcagtgac
    22381 gaactccgga ttcgcgccag taatctggag ctggcaggcc tggagggcat acgcccgctg
    22441 gccgcgaaac tttcacctgc actgggtgat gtttggcgct ccacacaacc gagcggcaag
    22501 attaacactc tggcgctgga tatcccgctt caggcggcag acaagacccg ttttcaggca
    22561 tcgtggagcg atctggcctg gaagcaatgg aaattattac cgggtgcgga acacttctcc
    22621 gggacgcttt ccggcagcgt tgaaaatggt ttgcttaccg cgtcgatgaa gcaggcaaag
    22681 atgccttacg aaacggtatt ccgtgcgcca ctggaaatcg ccgacggcca ggcaactata
    22741 agctggctga acaatgacaa aggtttccag ctggatgggc gtaatattga cgttaaagcc
    22801 aaagccgtcc atgcgcgcgg cggttttcgt tacctgcaac ctgctaacga tgaaccctgg
    22861 ctgggtattc tggctggcat cagtaccgat gatggttcac aagcctggca ctatttcccg
    22921 gaaaacttga tgggtaaaga cctggttgat tacttaagtg gcgcgattca gggcggtgaa
    22981 gcggataacg cgacgctggt ttatggtggc aatccgcaac tcttccccta taaacacaac
    23041 gaaggtcagt ttgaagtgct ggtgccgctg cgcaacgcga agtttgcctt ccagccggac
    23101 tggcctgcat taactaacct tgatattgaa ctggacttta ttaacgacgg tttatggatg
    23161 aaaaccgatg gcgttaatct gggcggcgtg cgcgcgagta atctcaccgc agtgatccct
    23221 gactactcga aagaaaaact gctgattgac gctgacatta aaggtccggg taaagccgtt
    23281 ggcccttact ttgatgagac accgctgaaa gattctctgg gtgcgaccct gcaagaactc
    23341 cagctcgacg gcgatgtgaa tgctcgctta catcttgata tcccgctgaa cggcgagctg
    23401 gtaaccgcga aaggtgaagt gacgctgcgt aataacagtc tgtttatcaa accactcgac
    23461 agcaccctga aaaatttgag cggtaaattc agctttatca atggcgatct gcaaagtgaa
    23521 ccactgacag caagctggtt taatcagccg ttgaacgtgg atttttccac caaagaaggg
    23581 gcaaaagcct accaggttgc ggtgaatctc aacggtaact ggcaaccggc gaaaaccggc
    23641 gttctgcctg aagcggtgaa cgaagcattg agtggcagcg tggcgtggga tggtaaagtg
    23701 ggcattgatc tgccttatca tgctggcgcg acctataacg tagagctgaa cggcgatctg
    23761 aataatgtga gtagtcactt accttcaccg ttagccaaac ctgcgggtga accactagcg
    23821 gtaaacgtta aggttgatgg caatctcaac agctttgaat taaccggaca ggctggtgcg
    23881 gataaccatt tcaatagccg ctggttgctt ggtcaaaagc tgacgctcga tcgtgctatt
    23941 tgggcggcag acagtaaaac gctcccgccg ttgccggaac aaagtggcgt tgaactcaat
    24001 atgccgccga tgaatggtgc cgagtggctg gccctgttcc agaaaggcgc tgcggagagt
    24061 gtcggtggtg cagcgagttt cccacaatac ataacgttac gtacgcctat gttgtcactg
    24121 ggaaatcagc aatggaataa cctgagtatt gtttcgcaac cgacggcaaa tggcaccctg
    24181 gttgaggcgc aagggcgtga aatcaacgcc acgctagcga tgcgtaataa cgcgccgtgg
    24241 ctggcgaata tcaaatatct ttattacaac ccgagcgtgg cgaaaactcg tggtgattca
    24301 acgccgtcat cacctttccc gacaacggag cgcattaact tccgtggctg gccggacgca
    24361 caaatacgat gcacagagtg ctggttctgg gggcaaaaat tcggtcgcat tgacagtgat
    24421 atcaccattt ctggcgatac gttaacgctg accaatggac tgattgatac tggtttctcg
    24481 cggcttactg ccgatggtga atgggttaat aatccgggga atgaacgtac ctcgctgaaa
    24541 ggaaaactgc gcgggcagaa aattgatgcc gccgcagaat tttttggtgt cacgacgccc
    24601 atacgccagt cgtcatttaa tgtggattac gatttacact ggcgcaaagc accctggcag
    24661 ccagatgaag cgacgttgaa tggcatcatt catactcaac tgggtaaagg cgaaattacc
    24721 gaaatcaata ccggacatgc cgggcaattg ctgcgcttat tgagcgtaga tgccctgatg
    24781 cgtaagctgc gttttgattt cagagacact tttggcgaag ggttctattt tgactccatt
    24841 cgcagcaccg cgtggattaa agacggggtt atgcacaccg acgacacgct ggtggatggc
    24901 ctggaggcgg atatcgccat gaaagggtcg gtaaatctgg tacgtcgcga cctgaatatg
    24961 gaagcggttg tcgcaccaga gatttctgcg acggtgggcg tggctgcggc ttttgcggtt
    25021 aaccccattg ttggcgcggc agtttttgcc gctagtaaag tgctggggcc gctgtggagc
    25081 aaagtctcca ttttgcgcta tcacatttcg ggtccgctgg acgatccgca aatcaacgaa
    25141 gtgttgcgcc aaccgcgtaa agaaaaagcg caatgatttg acgagggcgc gtaattgccc
    25201 caatctcata tgataatcgt tgccaaaggc caacgagcca gaacataacc gtaggccgga
    25261 tagggcgttc atgccgcatc cggcagccgt aaaaaatcct ctactgcagt aactaacgag
    25321 tagcaaaaac gatgagtctg aacctggtaa gtgaacaatt gctagcggcg aacagcctga
    25381 aacatcagga cttgttcgcg atcctcggtc aactggccga acgtcgcctt gattatggcg
    25441 atctctattt tcagtcgagc tatcacgaat cctgggtttt agaagaccgc attattaaag
    25501 atggttctta caacatcgat cagggcgttg gcgtgcgtgc aatcagcggt gaaaaaaccg
    25561 gatttgctta cgctgaccaa atcagcctgc tggcgctgga acagagtgcg caagcggcgc
    25621 gcaccatcgt ccgtgatagt ggtgatggca aagtacagac gctgggcgcg gtagagcata
    25681 gcccgttgta tacctcggta gatccgctgc aaagcatgag ccgtgaagag aagctggata
    25741 tcctgcgtcg cgtcgataag gttgctcgcg aagcggacaa gcgcgtacag gaagtgactg
    25801 ccagcctcag tggtgtctat gaattaattc tggttgcggc caccgacggc acgctagcgg
    25861 cggatgtccg tccgctggtg cgtctttccg tgagcgttct ggtcgaagaa gatggcaaac
    25921 gcgaacgcgg tgccagtggc ggcggcggtc gttttggtta tgagttcttc cttgccgatc
    25981 ttgacggcga agtccgtgcg gatgcatggg caaaagaagc agtgcgtatg gcgctggtca
    26041 atctttctgc cgttgctgca ccagcgggca ccatgccggt agtacttggc gcaggttggc
    26101 cgggcgtgct gttgcatgaa gcggtaggtc acggtctgga aggcgacttc aaccgccgcg
    26161 gcacttcagt atttagtggg caggtcgggg agctggtggc ttcagaactg tgtaccgtgg
    26221 ttgatgacgg cacgatggtc gatcgccgtg gttcggtggc gattgatgac gaaggtacgc
    26281 caggccagta caacgtgctg attgagaacg gcattctgaa aggctacatg caggataaac
    26341 tcaacgcgcg tttgatgggg atgacgccga ctggcaacgg tcgccgtgaa tcctacgccc
    26401 atctgcccat gccgcgtatg actaacacct atatgctgcc gggtaaatcg accccgcagg
    26461 aaattattga atccgttgag tacggtatct atgcaccgaa ctttggtggc ggtcaggtgg
    26521 atatcacctc cggcaaattc gttttctcca cttcagaagc atatctgatt gaaaacggta
    26581 aagtaacgaa gccggtgaaa ggcgcaacgt tgattggttc cggtatcgaa accatgcagc
    26641 agatttcgat ggttggcaac gacctgaaac tggataacgg cgtgggtgtc tgcggtaaag
    26701 aagggcaaag tttgccggtt ggcgtgggcc agccaacgct gaaagttgat aacctgactg
    26761 ttggcggtac tgcgtaataa tttcattatt ttcagaagga taattaatct ttctacgtgc
    26821 acgaacggtc ccctcgctcc tctggggtta gggtgagggg aacccgttgg cacaggtttg
    26881 tagaacgtaa cagtacaata tgaattactt ctctttcccg cgcccgtgca tctcctgaaa
    26941 caatttaccg acctcaacaa aataatccgt cagcgagttg atcacgacct gtaccttcag
    27001 cggcagctta tctttttcgg tatataacgc ataaaccggg cgtggatctg actgataacg
    27061 cggcagcagg atctccagct caccgcgatt gatctcgttg atcacccaca tcagcggcac
    27121 gtaggcgatc ccggcacccg ccgtcagcca gcgcaccagc gtcatcggat cattagtcac
    27181 aaatcttcct tgtgggatca ggcgagtcga gatcccttcc ggtgcgatca gttcaaattc
    27241 attgtcgggc cgcacgctgt attcaagcca tgaatgacta ctcaaatcgg cgggtttttc
    27301 cggtatgccg tattgtgtga gatagctttt cgcggcgcac accaccatcg gcatcgcgcc
    27361 cagacggcgg gaaaacaggc tggaattccg gtaaccagat tgacgctcaa acctgggtat
    27421 tctttcagca tttttgctgt cagcccggcg agaacatttt gtgccatagt tgaagaacag
    27481 ccaatgcgca gcgtcccgat gggggtgtta ttgaaggcat acagttgctc atgaacatcc
    27541 tgcacttcat gaagcatccg acggcagccc tggtagtaaa ttctgccggc ttcggtcagg
    27601 ccaatgctgc gtgtgctacg gtttaacagc tttacctgca actcatcttc cagttttgac
    27661 accgtctgac tgatggacga aacgctcatc tgtagctgtc tggcggcggc ggtaaaagag
    27721 ccaaattcaa ctactttggc aaacaccgac atgcgtttta gtcgttccat tattcactct
    27781 gacttaaaa
//
LOCUS       DAZPAC010000038        27786 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZPAC010000038 DAZPAC010000000
VERSION     DAZPAC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27786
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.038"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..317
                     /gene="tnpA"
                     /locus_tag="ACHUPM_001314"
                     /pseudo
     CDS             1..317
                     /gene="tnpA"
                     /locus_tag="ACHUPM_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            377..451
                     /locus_tag="ACHUPM_001315"
                     /pseudo
     CDS             377..451
                     /locus_tag="ACHUPM_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(448..1771)
                     /locus_tag="ACHUPM_001316"
                     /pseudo
     CDS             complement(448..1771)
                     /locus_tag="ACHUPM_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1771..3387)
                     /locus_tag="ACHUPM_001317"
     CDS             complement(1771..3387)
                     /locus_tag="ACHUPM_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR0869146.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3683..4399
                     /locus_tag="ACHUPM_001318"
     CDS             3683..4399
                     /locus_tag="ACHUPM_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0869147.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4396..6057)
                     /locus_tag="ACHUPM_001319"
     CDS             complement(4396..6057)
                     /locus_tag="ACHUPM_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR0869148.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6253..6681)
                     /gene="ibpB"
                     /locus_tag="ACHUPM_001320"
     CDS             complement(6253..6681)
                     /gene="ibpB"
                     /locus_tag="ACHUPM_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR0869149.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6793..7206)
                     /gene="ibpA"
                     /locus_tag="ACHUPM_001321"
     CDS             complement(6793..7206)
                     /gene="ibpA"
                     /locus_tag="ACHUPM_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR0869150.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7200..7272)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7512..7844
                     /locus_tag="ACHUPM_001322"
     CDS             7512..7844
                     /locus_tag="ACHUPM_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR0869151.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7846..9060)
                     /locus_tag="ACHUPM_001323"
     CDS             complement(7846..9060)
                     /locus_tag="ACHUPM_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR0869152.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9161..10225
                     /gene="cbrA"
                     /locus_tag="ACHUPM_001324"
     CDS             9161..10225
                     /gene="cbrA"
                     /locus_tag="ACHUPM_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR0869153.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10222..11559)
                     /locus_tag="ACHUPM_001325"
     CDS             complement(10222..11559)
                     /locus_tag="ACHUPM_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0869154.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11634..12782)
                     /gene="dgoD"
                     /locus_tag="ACHUPM_001326"
                     /pseudo
     CDS             complement(11634..12782)
                     /gene="dgoD"
                     /locus_tag="ACHUPM_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12779..13396)
                     /gene="dgoA"
                     /locus_tag="ACHUPM_001327"
     CDS             complement(12779..13396)
                     /gene="dgoA"
                     /locus_tag="ACHUPM_001327"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR0869155.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13380..14258)
                     /gene="dgoK"
                     /locus_tag="ACHUPM_001328"
     CDS             complement(13380..14258)
                     /gene="dgoK"
                     /locus_tag="ACHUPM_001328"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR0869156.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14255..14944)
                     /gene="dgoR"
                     /locus_tag="ACHUPM_001329"
     CDS             complement(14255..14944)
                     /gene="dgoR"
                     /locus_tag="ACHUPM_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR0869157.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            14913..15545
                     /locus_tag="ACHUPM_001330"
                     /pseudo
     CDS             14913..15545
                     /locus_tag="ACHUPM_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341111.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15591..16403)
                     /gene="yidA"
                     /locus_tag="ACHUPM_001331"
     CDS             complement(15591..16403)
                     /gene="yidA"
                     /locus_tag="ACHUPM_001331"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR0869158.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16518..16916)
                     /locus_tag="ACHUPM_001332"
     CDS             complement(16518..16916)
                     /locus_tag="ACHUPM_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR0869159.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17156..19570)
                     /gene="gyrB"
                     /locus_tag="ACHUPM_001333"
     CDS             complement(17156..19570)
                     /gene="gyrB"
                     /locus_tag="ACHUPM_001333"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR0869160.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19599..20672)
                     /gene="recF"
                     /locus_tag="ACHUPM_001334"
     CDS             complement(19599..20672)
                     /gene="recF"
                     /locus_tag="ACHUPM_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR0869161.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20672..21772)
                     /gene="dnaN"
                     /locus_tag="ACHUPM_001335"
     CDS             complement(20672..21772)
                     /gene="dnaN"
                     /locus_tag="ACHUPM_001335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR0869162.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(21777..23180)
                     /gene="dnaA"
                     /locus_tag="ACHUPM_001336"
     CDS             complement(21777..23180)
                     /gene="dnaA"
                     /locus_tag="ACHUPM_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR0869163.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23477..23557
                     /locus_tag="ACHUPM_001337"
     CDS             23477..23557
                     /locus_tag="ACHUPM_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869164.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            23787..23927
                     /gene="rpmH"
                     /locus_tag="ACHUPM_001338"
     CDS             23787..23927
                     /gene="rpmH"
                     /locus_tag="ACHUPM_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR0869165.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            23944..24303
                     /gene="rnpA"
                     /locus_tag="ACHUPM_001339"
     CDS             23944..24303
                     /gene="rnpA"
                     /locus_tag="ACHUPM_001339"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR0869166.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24267..24524
                     /gene="yidD"
                     /locus_tag="ACHUPM_001340"
     CDS             24267..24524
                     /gene="yidD"
                     /locus_tag="ACHUPM_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR0869167.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24527..26173
                     /gene="yidC"
                     /locus_tag="ACHUPM_001341"
     CDS             24527..26173
                     /gene="yidC"
                     /locus_tag="ACHUPM_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR0869168.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26279..27643
                     /gene="mnmE"
                     /locus_tag="ACHUPM_001342"
     CDS             26279..27643
                     /gene="mnmE"
                     /locus_tag="ACHUPM_001342"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR0869169.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6685 a         7520 c         7090 g         6491 t
ORIGIN      
        1 atgacgcgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat tttgaagctg
       61 aactggttta aatggatggc gaaccagatc acgtccattt gttaattaac tatcctccca
      121 aactggcgat atccagtctg gtaaacagcc tcaaaggcgt atcgggtagg ttactgcgac
      181 gagatcgacc agatattgca gtcaggtatt actacaaagg cgttttgtgg agtcctggct
      241 attttgccaa tagctgcgga ggtgcgccaa tatccgtcat ccgccaatac attgaacaac
      301 agcaaacacc tggttaggtg gaaaaccgcg ccttatatcc ccgccctgaa ggacggggtt
      361 ttacggcgca ccggataaat ttggttatgc gccgtcagcc gtgctaaaga atacggacca
      421 gcacccaacg gatgccagtc cacacaatta acgtaactct ggccagtacg ctttgttggc
      481 ctccagcaac tcatccagaa tagctttggc gaccgaggcg ctcggtaccg ttttcgataa
      541 cgtaatcgcc tgccacaggt gctgatatga acgctgttcc caggcgtcca cgaccagttt
      601 ttccaccgcc acttgctgac tcattaaccc tttctgaaac tgcgggatat cgccgaccac
      661 cagtggttct ggtccgttgt gcccgaccag acacgggatc tcgaccatcg cttcatcatc
      721 aaagttatgg attgccccgt tgttaggcac aatcagcaac atccgctcct gagtgttgaa
      781 ggcaattgcc gccgccagat cgacgatgta tgacgcatgt tcgtcaatct ccagcttggc
      841 cgccgctgaa tttcctgccg ctgtaatggc gcggcaagca tcgaaaacct gtttctcgcg
      901 atgttccatt cacctcattc gcgcgggtat gttcagggtt ggaatgctga acaacataat
      961 ccgggaagag ataatatttc agataggtgt tcggcaatgt atccgggtcc agtgcctgga
     1021 catcccgcgc ttttgcatag gtgtcgttcc agctcgcttc agtatgtgta tcttgctgcg
     1081 gtggaacata accgtgcttc gagacatatt gccgcagctg gggcatcagg tcgttgccct
     1141 gcaaatcttc aattgatgtc caccagccaa agtggttcag gccgtagtag cgcacgcgca
     1201 tctgtttgcg atcttgcagc ccaacaattt gcgccatccg gctttcaata ccgattggca
     1261 tgtcacagat gttgaggatt ttcgcattcg ggcgcagacg acgcgtggct tctgcgacaa
     1321 tggctgccgg gttggagtag ttgagcatcc aggcatttgg tgaatatttt tccatataat
     1381 ccaccagttc caggacgccg ccaatggaac gcatgccgta cgctattccg ccgggtccgc
     1441 aagtttcctg accaacaaca ccgtggcgca gtgggatttt ttcatccagt tcgcgcatcg
     1501 ggtacttgcc gacgcggatg tgcgccatga caaaatcaac gtcgctgaac gccacttcag
     1561 gatcggtggt gtaactaaac gcaatgtccg gcgctttctc tttaaggatg actttacagg
     1621 cttcggcaat cacttcctgc cgcgcgccat cgttatcata aaatttcagt gcacgaagcg
     1681 ggaaacgctc ctgattcgcc aggagcatca acacgatgcc tggcgtaaag gtgcttccac
     1741 cgcctgcgac aaccactgag aatttggtca ttatactgcc tccgtaatgg caacattttc
     1801 tgctgattga tgagaattaa ttaagctatc gagctgttca cgcagctggg atacatgcag
     1861 accaatgatc acctgaatgg catcgccact acggaagacg ccgtgcgctc ccagcttttt
     1921 aaagacttcg tcatccagcg tttgtgacat gtcatgcagt gcaatgcgta gacgcgtcgc
     1981 acaattgtta atgctggaga tattgccgac cccgcccagg gcttgcagga taccggcagc
     2041 ctgatccagc tctttttttg gctctgccgc ggtggtttgg cctcgcgagg ctttgtattc
     2101 ggcttttgag tagagtttca cttccgcatc ttcacgtccc ggcgtgcaca tgttgaactg
     2161 cagaatcagg gtgcggaaaa ccacgaagta cagcagggta aagcacaacc caatggcgat
     2221 ttgggtcagc atcatatccg cgtggttgct gaacatcggg atccagtttt gcggtaaaac
     2281 ctggtcaatc agacctccgc ccatgttgcc caccacacca aagagataca tcacggtcga
     2341 cattgaggcc gcaagcacag cgtgtaccgc aaacagcaac ggtgaaatga acaggaaggt
     2401 aaattccagc ggttcggtaa ttcccaccag catggcggtt aaggttgcgg gaatcagcaa
     2461 gcccgccact tttacccgat tttccggtgc ggcagtgaag tacatcgcta aagaaatgcc
     2521 cacggcacca aagatttttg agttaccgtg cagggcaaaa ccgccttccg ggaacaacga
     2581 tttcagcggc tcggcgctca aactgaactc ttgcagatgc tgcgcccagt acatctgaat
     2641 gccgccttca acagctgccg gacccaagat aaacggtccg tagatgaagt ggtgtaaacc
     2701 ggttgggatc agaatacgtt cgaggaaggt gtaaacccag accccaagcg cacccgccga
     2761 acgcaggaac gcttgcagag attcaatccc cgtttgtact tttggccagc cgagcaatgt
     2821 cagccaggca caggggatca tcaccaggaa ggcgataatc accacataag acgtcccctg
     2881 gaaaatgccg agaaaaaccg gcagtttttt atcgaacaga cggttatgca gcgccgtcac
     2941 aatgccggaa atgataattg cgccgataat gctggtatcg agggttttaa tcccggccat
     3001 cattgtcaga ccgctacctg ccaccgcgtc ctgagtgaaa tcgacgccga agtagcttcc
     3061 ccaggtcatt cccatcgcgt tgatgaaata gttccaggtc aggaaactca ccatcaccgc
     3121 cagacaagca cgtccctgcg cttgcttagc aaggccaatg ggtaaaccga cagcaaaaat
     3181 cagcggcata ttacggaata ccgtccaacc gccctcttca ataatgtgta cgatttgcgc
     3241 gaataaactg ttcggatcgg tcagtgattc cccgacaaac atcgggtttt gcagcaagat
     3301 ggcaagaccc accacaatcc cggcgaaggg aaacagcagc actggcgtaa acatcgcgcc
     3361 gccaaagcgt tgaatttgac tgagcatttc cacatcctct tttctcaatt ctgtaagtag
     3421 gaagcctgat tggttatgcg gcaggcctgg agcaaggata ggaagatgcg atcggcagaa
     3481 catgacgacc caatgggatt cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct
     3541 acgttttcgc gaaaaagtag acatctgtga gcacaggtac acagcatcga acacatcttt
     3601 aaaaaaaaga tgttttttca atcgattaag cagaacttgt gtgcgcatta cccgggcttg
     3661 caggcaaaaa agagatctag agatgatcta caaaagcatt gcggagcggt taagaattcg
     3721 acttaactcc gcagatttca cgctaaacag ccttcttccg ggtgaaaaaa agctggcgga
     3781 agagtttgcg gtatcgcgga tgaccatccg taaagccatt gacctgctgg tagcgtgggg
     3841 gctggtggtc cgccgccacg gtagtggcac ttacctggtg cgcaaagatg tactgcatca
     3901 aaccgccagt ctgaccgggc tggtggaggt gttaaaacgg cagggaaaaa ctgtcaccag
     3961 ccaggtgctg atttttgaaa tcatgcctgc gcctccggcc attgccagcc agttacggat
     4021 tcaaatcaac gagcagatct acttctcccg tcgcgttcgt tttgtggaag ggaaaccgct
     4081 gatgctggaa gacagctata tgccggtaaa actgttccgt aatctttcgc tgcaacatct
     4141 ggaagggtcg aagtttgaat atattgaaca agagtgcggg attttgattg gcggtaatta
     4201 tgaaagcctg acgccggtgc tcgccgatag actgctggcg cggcaaatga aggtagcgga
     4261 acacacgcca ctgctgcgga tcacctcgtt gtcatatagc gagagcgggg agtttttgaa
     4321 ttattcagtg atgttcagaa atgccagcga ataccaggtg gagtaccatt tacggcgact
     4381 ccacccggaa aagagttaac cgatactcca gaagagcacc gccagtaatt ggggggtgat
     4441 aatgcgcagg aacatcacta acggatagac agtggcgtaa gagagcgccg ccgcaccgct
     4501 agttggatga agattattag caaacgccag cgccggagga tcggtcatgg aacctgccag
     4561 catcccgcac atggtcaggt aattcatttt ggctaacatc cgcgccagaa tgccaacagt
     4621 aatcagcgga acggcggtga tcagggcacc ataaccaatc cagcttagcc cttcgccatt
     4681 gaccagggta ttcacaaaat ccccaccaga tttcagacca acgaccgaga ggaacagcac
     4741 gatccccagc tcccgcagcg cgaggttggc acttggcggc ataaaccagt acagcttgcc
     4801 gatactgccg atacgcccga ggatcaacgc cataatcagc agaccgcccg ccagccccag
     4861 tttcaacgcg gccgggaatc ccggcacaaa gacgggaata gaacctaaca atacgccaag
     4921 cccgatgcca ataaacactg gcagcatctg aacctgttgc agtttttgct gcgcattccc
     4981 cagcacattg gcaacggcat caattgcgga cggacgcccc accaggttga ggatatcgcc
     5041 gaactgcagg ctgatatcgc cactggcgac cagttcgacc ccggcacggt tcaggcgcga
     5101 gataacaacg tcatagcgtt ctttaaagtg caggtcgcga atacgttttc cgagcacgtt
     5161 ttcattggtg accaccacac gctcgacgcg caaatcagtg tctttcgtgg acagcgaagt
     5221 atcgacctcc tgaccaatca ccagttgcgc attatgtaaa tccgctggct gacccaccag
     5281 atgcagcaaa tcgcccagtt ggataatggt atctggcgaa ggaactttta gggtttcttc
     5341 gcgtttcaga cgcgagcaga taattttgtc gccgttgaga atcggtacat ctttaatggc
     5401 taaatcatgc aggttagggt tctcaacgcg aatattgata gtcttgatca gcgcgccgcc
     5461 attggtgcgt gaagactcgt gctgctgagc ttctgtctcg acattgacgc ggaaaataac
     5521 ccgcaacatc cacatggtga acaaaatccc gcaaatgccg aatggatacg ccatcgcgta
     5581 actcatcccc atctgatcga ccatttccat tggtgtaccc aggtcgcgca aaatctgctg
     5641 ccctgccccc agcgctggcg tattggtaac cgcaccggag aaaatcccca gcactaccgg
     5701 cagtggaata tcaaacagtt tatgcaggat ggcggtaacc agaccaccga tgatgacgat
     5761 cagaacagca aacaggttga ggcgtaatcc ggagacgcgc aatgaggcaa agaagcccgg
     5821 ccctacctga atcccgatgg tataaacgaa caggatcagg ccaaattcct gaataacatg
     5881 cagcatatcg ctacttaatg tcatccccgc ctgagaaaca aaatggccga cgatgatccc
     5941 accaaacagc acgccgccaa tacctaatcc tatgccgcga aatttgacgt tgccgataaa
     6001 caaaccgacg actgccacca aagccagaat actgaccgtt aatgctatat cactcatcgt
     6061 ccatttctct tgagataaca ttttaattgc tacggattat ggctgaatcg gtgaagcaga
     6121 tttgccttat agcgcacatt attacgggaa agaacgtgcc gaaatatctt aaacagtcga
     6181 aatttaacgc attgttgaag aagaaagaca aaaaaagccc caccagaatg gcggggcaaa
     6241 gagaatagct agttagctat ttaacgcggg acgttcgctg atagcgatac gctgcgctgc
     6301 gatgggttca ggctcattac gaattaaatc aatatgcagt aaaccgttta cgaaagttgc
     6361 gccagagact tccatatttt cagccagcgt aaagctcagg ctaaatggct gattcataag
     6421 cccttgatgc agccattttt tctcttcttt tggctgctcc ggcgtgcctt ttacgctcag
     6481 gcgcgtacct tccagttgaa tctctaaatc ttcctgacgg aaacctgcca gcgcaagggt
     6541 aatgcggtag tggttatcgt cgcttttctc aatgttgtac ggcgggaagc tctgactttc
     6601 accggcgttt tgcagtgcgt tggccagttt gtcaaaaccg atccattgac gcatcagtgg
     6661 ggataaatcg aagttacgca tagtcatttc tccttctaag aagcgagtaa gtacctgcaa
     6721 atccgaagat tcgcatatgc tccctgacgg cgagcatgga gatgtcaggc cgcgccaggc
     6781 ggccttaagg aattagttga tttcgatacg gcgcggtttt ttcgcttccg gaatcacgcg
     6841 ttcgagatcg atatacagca aaccatttac caggttagcg ccacgaacat gaatgttctc
     6901 agctaactgg aatttgcgtt caaagttgcg ttcagcgatg ccctggtaca gataggtgcg
     6961 ctctttttgt tcgtcggcgt gagcaccttt caccaccagc agattatcct gggcggtaat
     7021 ttccagttcg ctctcagcaa aaccagccac agcgatagca atgcggtaat ggttttcgtc
     7081 taccagttca acgttatacg gagggtagcc gccattactc tggctctggt tgttttctaa
     7141 gtggttaaac aaacggtcaa atccaatagc agaacggtaa agcggggata aatcaaagtt
     7201 acgcataatc aatagctcct gaaatcagcg agaatgtaag accttccaca atggacaggt
     7261 caggtagcca gaacacccat caggcgcgtc ctcatcgact acgatgtaaa aatgggtctg
     7321 gaaatgaact tttcaagcct tatcagcgga cttttttttg cagtttatgg tctattgcat
     7381 agactgaggg ggcagcacga aacgttaaag tgtgatagcc gccacagagc gacgtaatga
     7441 tgatgttatt ttcagcaagg gatcgctata actcatcagg ccaacaccca cgatcaacag
     7501 atgaatttat tatgataaga aatgtgttgt tagcgttcat gatatgcagc ggaatggcat
     7561 tactcggagg atgctccagc gtgatgtccc ataccggagg gaaagaagga acgtatcccg
     7621 gcacccgcgc cagcgccacc atgattggcg atgatgaaac caactggggc actaagtcgc
     7681 tggcgattct cgatatgccg tttaccgctg tgatggacac gcttttatta ccgtgggatg
     7741 tgttccgcaa agacagctcg gtgcgctcac gcgttgaaaa aagcgaagcc aacgcgcaag
     7801 ccaccaatgc ggttattcca ccggccagaa tgcctgataa ctgatttagc gatcagtttc
     7861 ggtgatccac agctggccac cttccatcca cgccagccat tgaccatccg gcgagaagac
     7921 cacggcgtcc gcagaaggtg gattggcgtg gttttcggtt aaatactcaa cctcgccgct
     7981 ttgcgcatgg gcacaagcaa ttcgattatc cagcacaaag cccaaccatt ctcctgacgg
     8041 atgccagtta aatgcagact gaatatccgt tttgttatgg gttaactggc gcggctcgcc
     8101 gccctgtggc gagataagcc acagttgcac aatgccgtta tcatcacgca ttaaaaacgc
     8161 gatttgcgta ccctgcggat tacagcgcac ccagtggcgc gggacgttga ctaaccccgg
     8221 ataagcccga tggtgggtaa aggttaaacg tcgctgcacg acgccacgcg gtggcgcggg
     8281 cagggtggtt tccgttccac ttaacggcgc atcacctgcc gctttccagc cagcttcatc
     8341 ttgcggcaac tcaacgataa acagctccgg cactttctca ccctttggcg aaagtgtgtc
     8401 gccaataaac gccagcgcgt gatttcctac ccatccttct tcataagcac gattgatttc
     8461 atcactgcca ggctgcggcg tgggcgtggt tttactcacc agcacacacc agtggctgcc
     8521 gctgtattca cgcggatgct gcttttgtac gttgaccgga ccaaacggcg cagcaacacc
     8581 gacgtttcgc aaatccagcg ccggatcgag ttcatgcatt acatggtcgt tataggtaaa
     8641 gctcaccctt tcaccgttcg ggctaaagac atgcacatgg ctgccgccgc gcagcgctcc
     8701 tggcgtatac ggcgcggtaa tatccattgc atcgagattg ctcaccttgc cgccttcagc
     8761 aatgactccg cgccgatgat ggaagtcgta atgccatgtt tcatcaggat tttccgggcc
     8821 gtgaataaag acatatttct ctgacttcgg atgaacggtc accacgccga cgtgtgcgcc
     8881 ctgtgacgca cagtagataa cctcgacctc accggtatgg atattcacac gctcaatggt
     8941 ctcgccggta aacgacgccc cagaaggacg cacgtcaaat accagccact ggctgtcggg
     9001 cgtccaggta ttggtattgg tgagcaggtg attacgggga gcaaaggtta tctgtttcat
     9061 ggcgcggtcc tgatacaaaa cgggtccagc catcatactt cacaaggact tcactttcag
     9121 acaatagtct tttgccatcg tgatgaatgt gagtgaaaag atggaacatt tcgacgtggc
     9181 gattattggc ctcggcccgg cagggtcggc gttggcacga aagttagccg gcaaaatgca
     9241 ggtgatcgcg ctggataaaa agcaccagca tggtactgaa ggtttcagca aaccttgtgg
     9301 cggtctgctg gcaccggacg cgcagcgatc ttttattcgc gatgggctga cgcttcctgt
     9361 cgatgtgatc gctaatccgc agattttcag cgtcaaaacc gtcgacgtcg ccgcatcgct
     9421 cacgcgtaac taccagcgaa gctatatcaa tattaatcgc catgctttcg acttgtggat
     9481 gaaatcgctg atccccgcca gcgttgaggt ttatcacgac agcctgtgtc gaaaaatctg
     9541 gcgtgaggat gataaatggc atgtcatttt tcgtgcagac ggctgggagc agcatattac
     9601 cgcccgctat ctggtcggtg ccgatggtgc caactcgatg gtgcggcgac atctctaccc
     9661 ggatcatcaa atccgtaaat atgtcgcaat ccagcagtgg ttcgcagaga aacatccggt
     9721 gccgttctac tcctgcatct ttgataatgc gataactgac tgttattcat ggagtatcag
     9781 caaagacggt tattttatct ttggcggtgc ctatccaatg aaagacggtc agacgcgttt
     9841 cacgactctg aaagagaaaa tgagcgcctt tcagttccag tttggcaaag cggtgaaaag
     9901 cgaaaaatgc acggtgctgt ttccctcacg ctggcaggat tttgtctgcg gtaaggacaa
     9961 cgcctttctg attggtgaag cggcgggatt tatcagcgcc agctcgctgg aggggattag
    10021 ctatgcgctg gatagcgcag acattctgcg ttcggtgtta ctgaagcagc cagagaagct
    10081 caataccgct tactggcgcg ccacccgcaa actgcgttta aaactcttcg gcaagatagt
    10141 aaaaagccga tgcctgaccg caccggcttt aagaaagtgg attatgcgca gtggtgtggc
    10201 acatattcca cagttgaagg attagccaac gcgcttcaca tcgcccacca gcaggatata
    10261 agagagcgcg ccaatcaacg cgacggcgga gatataaacc agtgcaggtg cgaaaccgta
    10321 accctgcgcc aggtacccca ccaccagcgg aacggtgatg ccgcccagac caccggcgaa
    10381 gttaaacacg ccgccggtta aaccaatcag gcgcatcggt gccagagaag aaaccagcga
    10441 ccaggtaatt gaagcaaaac cgttaccgaa gaatgccagc gccatcaggc acataatcat
    10501 catcggatcg ttagtgtagt tagcgcccat aatgcaggtg gagatcagca agccgcagat
    10561 aatcggcgtt ttacgcgcaa agcccagtga aaagccctta cgtaccagca gatccgctac
    10621 ccagccagag agcaggacgc cgacaaacgc cgcgaggaat ggcaccgtgg tcataaagcc
    10681 tgctttcagc gccgtgattc ctttttcctg ggttaaatag ttcgggaacc aggttaagaa
    10741 aaaccacagt gtagaagcca ccgcaaattg cccaagatag acgccgatca gtttacgatg
    10801 gaacaccagt ttccagtctt tggctgttaa cggctgacgt gcctctttct tcactggcgc
    10861 atcaccatcc accagaccgc cgccatcacg aatgtaatcc agttcagctt tgctgatgcc
    10921 tttggtcagg cgcggcggct gataaacctt aaaccaaatc agcgaccaga taatgccgat
    10981 accaccagtc acaatgaaca cccagtgcca gctcaacatc tcctgaatcc agatcagcag
    11041 cggagtcaaa aacgccagac cgacaaactg accagacgta taaaaaccaa cggcggaggc
    11101 gcgttcatgt tccgggaacc agctggtcac catccggtta ttggtcggga aagcgggcgc
    11161 ttcgaaaata ccggttatcg cacgcagacc aattaatgac attaatcccg tggcaaagcc
    11221 ctggaataaa gtcgccactg accagccaaa tatcgcaata aaataagtca cgcgagaacc
    11281 tacgcgatct aaaaaccaac cgccggggat ctggcatagc gtataaagcc aggcgaaggc
    11341 cgaaaataca tagcccattt ccgctttcgt aatgccgaac tcttcctgaa tatgggcgga
    11401 agccacggcc aggttagcgc ggtcaacata acaaatgacc accgtaataa agatcatcac
    11461 cagcgtcaga taacgccgac gccccggctt tgctgcatta acgggaatat ccatagcgag
    11521 ctttctccag attttgggca tagcgaagcc gctcaccatg ccctgtaatt tacagagggt
    11581 tatatttttg tattgctgtt ttagtgcccg atgaggggct tacgtggcag gaattaccac
    11641 tctgctacgc tgttatcttc atgacgccag agtggattac gccagtccgg ggcattttta
    11701 ctgaactcaa taactttagc ttcgtcgatt tccacgccta agcccggttt cgttagcggt
    11761 ttaaagaagc cgccaaccat gctgaagtct tctttgtttt tcacaaagtc gagtaactcc
    11821 gcgcctttgt tgtaatgaat tcccatactt tgttcctgaa gtaccgcgtt ataggaaaca
    11881 aagtcgatat gcagacaagc cgccagtgca atcggtccga gcggacagtg cggcgcaagg
    11941 gtcacgtcat aggcttctgc cattccggca attttgtagc attcggtaat accgcctgca
    12001 tgggagagat ccggttgtag aatcgaaata ccgcctgcct ccagcacgcg tttaaaatcg
    12061 aaacgtgaga acatgcgctc acccgccgcc agtggaatat gcgtttgtgc cgccaatttc
    12121 gggtagtatt cggcttgttc cgccagcacc ggctcttcaa taaacagcgg gcgatacggc
    12181 tccagttctt taatcagcac tttcgccatt ggcgcgctga cgcgaccgtg gaaatcaaga
    12241 ccaaactcaa tctgattgcc aaaagcttca cgaatttgtg ccacggtgtt gactgccgca
    12301 tctaccgcgc gggagttatc aattagcccc agttcttcac aaccgttcag tttgaaggta
    12361 tcgaagccga tttcgcgcag ggttttaata ccgtcgatca catccgccgg acgatcgccg
    12421 ccgacccaac tgtaggcttt aattttgtcg cgaaccaggc cgcccatcag ttgccagacc
    12481 gacgcattca gcactttacc tttgatatcc cataacgcct ggtcgatccc ggcaatggcg
    12541 ctcatcagga ttggaccgcc acgataaaat ccggcgcgat acatcacttg ccataagtca
    12601 ttgatgcgct atggatcctg accaatcaaa tagtcaccca gctcgtgaac tgccgcttcc
    12661 accgtgcggg cgcggccttc aatcactggc tcgccccagc cgacgacgcc ttcatcggtt
    12721 tcaattttca ggaacatcca gcggggaggt aaacgatacg tggtaatttt ggtgattttc
    12781 attgcactgc ctctcgatac gccttaacaa atgctgctgc ctgctgcgcg gtgcgttcta
    12841 cggactgccc ggcgcgatag agatcgctgc ctaagcccgc cccagcacaa cctgcgtcta
    12901 tccactgcgc caggttttct ggcgtcacgc cgccaacggc aaagactgcg atgtccgatg
    12961 gcaataccgc ttttaacgct ttgatgtatt gcggaccaaa agccgatgac ggaaatattt
    13021 tcagcgcctg cgcgcccgct tcgagcgcgg taaaggcttc ggtcgccgtc gcgcagccgg
    13081 ggcagacggt catgccgtag cccaccgcac ggcggatcac ttcactatgg atattgggcg
    13141 taacgatgag ctgacagccc atcctggcga gcgcatcgac ctgttcaggt ttcagtaccg
    13201 tacctgcgcc aatcaacgcc ttgtcgccat acgcatcaac gatggcggga atgctttgct
    13261 cccattgtgg ggaattcagc gggatttcaa ccgcgtcgaa cccggcgtca atcaccgcgc
    13321 caacatgcgc cagcgcctcg tcgggcttaa tgccgcgcaa aatggcgatc agcggaagtt
    13381 tagtttgcca ctgcatgagc gatgctcctt ataccagcct gaaatgccgt gtcgcccgcc
    13441 accgccgtca cgtcgcaacc catcgcctga aaggcttgct ggtagcgcgc ggtcaacgat
    13501 gttccggcga caagggtgat ggcgtgttga tgggccacat agtcgcgcat actggcgacc
    13561 tctgcgccaa tcaacaaacc agagagaaat tcactgacct gttcacgggg aagtgttccc
    13621 agcacatgcg aggcacgaac ttcaaaaagc tgcggcaata tggcgggcgt attaagacca
    13681 cgctcaaggc cagccgtgaa ggcatcggca gagttttcct gcggcggcaa acctgcacca
    13741 atcaatgagt gatttaacag taaatgatgt aattcaccgg tcatcacggt gcgaaaatcg
    13801 ttgatttgct ggctatcggc ctgcacccat ttgcaatgcg taccgggcat gacataaaga
    13861 gaggaaggag ccagagctcg cgcgccgatc aattgagttt cttcgccgcg catcacattg
    13921 tggttatcat catgagagac gcataatccg ggaataatcc agatattgtc gccaacagac
    13981 gttaattgtt cgccaataga cgaaaaatgg gcaggaacag ataaatacgg agcaactttc
    14041 cagccgacat tgctgccgac cattcctgcc attaccactg gcgtattctc ttcacgccag
    14101 tcggtcgtga cttctgctaa caccgcagcc ggagattttc cgttcaggcg cgtgacgcct
    14161 gcttctgatt gcctgctctc caggcagtgg tcgccctgat aaagccaggc gcgcagattg
    14221 gtcgatcccc agtcaattgc gatgtagcga gctgtcatgt gatttccttt aaccttcgtg
    14281 tcgagctggc gatcatggta agcgccgcct gctctgccgc atcgccgtcc tgatgccgta
    14341 tcgcatcgaa cagcgcctta tgttcctgga gcgtttgcgg catgttggcc tcatcgccca
    14401 tccaggttcg ttcaaaaacc gcccgctgca gcgaactgat cgcaatgcta agttgctgta
    14461 acaccgggtt atgcaccgac tgcagcaccg cctcgtggta gcgaatatcc gcttcgttaa
    14521 acgcttcgcg gtcctgattg ttggcaatca tctcgttcag cgccgattca atctgcgcca
    14581 gatcgctgga ggtcgcgcgc tctgctgccc aacgggcaat cgccggttcc accagatttc
    14641 gcacttcgct catggcactg ataagccgtg ggtcgtagtc gttttccagc acccattgca
    14701 gtacgtcagt gtcgaggtaa ttccactggt tacgcggtgc cacaaacgcg ccgcgataac
    14761 gtttcatttc aatcagccgt ttcgccatca acgaacggaa cacctcacgg atgatgttgc
    14821 gcgaggttgc aaactcctca cagagttccg cctcagccgg aagcggcgag cctggtacgt
    14881 atttgccgtg aacgatctgt ttacccagcg taatgacgat gcgatcggtt ttattgagag
    14941 tcatggagag tccttgtgct ctgtatgttc ttctctactt taccccgatc gatgcataat
    15001 caggaaagta tcaatcgcag tcagacacca tcacggggct atcgcaggca aaggatagta
    15061 atggaacaaa aggctatgtt tttgtagggg aatcgctgga ttaccttatc acagatggtg
    15121 ccgatgacat cgttaagatg ctcaaggatc cagctcttaa ccggcacaat attcaggttg
    15181 ctgatgacgc aagatttgtt ttaaatgcgg ggaaaaagaa atttaccggc acaatatcac
    15241 tctattacaa ctggagcaca gaagaagaaa aagcacgagt gacacaatat cgttttgctt
    15301 gtggtgttca gcactgtacc cggacagtag aaaacctgga aggtactatc catgagaaaa
    15361 ataaaaacat ggattactca aacgtaatgg ctttctacca tccatttaaa gtgcgatttt
    15421 atgaatacca ttcacccaga ggcattccgg atggtgtttc cgcagcatta ctgccagtga
    15481 ctgttacgct ggacatcatt actgcaccgc tgcaatttct ggttgtatat gcagtaaacc
    15541 aataatcagt aagcgggcaa acgcgtttat gctgtttgcc cgcccacaga ttaattcagc
    15601 acatacttct caatagcaaa cgccacgcca tcttcaaggt tagatttggt gacaaagttc
    15661 gccacttctt tcaccgaagg aatagcgtta tccatcgcca caccgacgcc tgcatattca
    15721 atcattgcga tgtcgttttc ctgatcgcca atcgccatga tttcttccgg tttaatacct
    15781 aacacgtcgg ccagtgattt cacacccgta cctttgttaa cgcgtttatc gaggatttcg
    15841 aggaagtacg gcgcactttt cagcacggta tatttctctt tcacttcctg cggaatacgc
    15901 gcgatagcct ggtcgaggat ggcgggttca tcaatcatca tcactttcag gaactgggta
    15961 ttggggtcca ttttctccgc ttcgcagaac accagcggaa tagtggcaac gaaagattca
    16021 tgcactgtgt agtagctgat atcgcggttg gcggtgtaca gcgtggtgcg gtccagggcg
    16081 tggaaatgag aaccgacttc gcgagagagt ttttccagga aacgatagtc gtcatagctg
    16141 agagcagttt gcgccacggt gctaccatca gctgccttct gtactagcgc gccgttataa
    16201 gtaatgcagt agtcgcccgg ctgttccata tgcagctctt tcaggtagtt gtgcacacct
    16261 gcatacgggc gacccgtcgt tagcacgaca ttcacgccac gggcgcgagc tgcggcaatc
    16321 gcatttttaa cggcgggtga aatggtgtga tcgggcagca gaagggtgcc atccatatcg
    16381 atagcaatga gtttgatagc catgagttcc ccaggtagat tggttcctga cccatgctaa
    16441 cgcgattccg ctcaaaaatc agtacaacac ccgagggaaa agggggatgc aacgcgggtt
    16501 gcatgctccc catttgctta gcggaagagt ttccctttca gcagttccat acctgcggaa
    16561 agcagatcgt tattggcttg tggtgacact tcaccttgcg gtgagagcgc atcaataatc
    16621 ttcggcaatt gctctgccag taaactggaa gctgtactgg tatccacgcc aagtttttgc
    16681 ccgagatcgg acaccgcatt tgtgccgagc gccgattcca gttgctcgcc actaaccgat
    16741 tgattgccct gttgattact cagccaggtt gagagaatgg cccctaagcc gccactttgc
    16801 agtttttcca gcagcacctg aatgccgccc tgctcctcaa cccaacttaa aatagcctga
    16861 tatttccccg catcgccttt cagaaaggca ccgacaactt catcaaaaag ccccatgata
    16921 atcacctgta aagcgttacg tgttgaccca aaaagtatag atttgcggga tgataattgc
    16981 ggattgcaga aataaaaagg gcggagatga tctccgccct tttcttatag cttcttgccg
    17041 gatgcggcgt gaacgcctta tccggcctac aaaatcatga aaattcaata cattgcaaga
    17101 ttttcgtagg cctgataagc gtagcgcatc aggcacgctc gcatggttag cgccattaaa
    17161 tatcgatatt cgccgctttc agggcgttct cttcaataaa cgcacggcgc ggttcaacgg
    17221 cgtcgcccat cagcgtggtg aacaactggt cggcagcaat cgcatcttta acggtaacgc
    17281 gcagcatacg acggctttcc gggtccatgg tggtttccca cagctgttcc gggttcatct
    17341 cgcccagacc tttataacgc tggatggaga ggccgcgacg ggactctttc accagccagt
    17401 ccagcgcctg ctcgaagctg gctaccggct gacgacgctc gccacgttcg ataaacgcat
    17461 cttcttccag caagccacgc agtttctcac ccagcgtgca gatacgacga tattcgccac
    17521 cggtgataaa ctcgtgatcc agcggatagt cagtatccac accgtgggta cgcacgcgaa
    17581 caatcggttc aaacaggttt tgttcggcat tggtgtggac atcaaacttc cactggctgc
    17641 cgtgctgttc tttgtcgttc agttcgctga ccagcgcgtt cacccagcgg gtaacggtct
    17701 gctcatcaga gaggtcggct tccgtcagcg tcggctgata gataagctct ttcagcattg
    17761 ctttcggata acgacgctcc atgcggttga tcattttctg cgtcgcgttg tactcagaca
    17821 ccaatttctc taacgcttcg ccagccaatg ccggtgcgct ggcgttggtg tgcagcgttg
    17881 cgccatccag cgcgatagag atctggtact gatccatcgc ttcgtcgtct ttaatgtact
    17941 gttcttgctt gcctttcttc actttgtaca gcggcggctg agcgatgtag acgtgaccgc
    18001 gttcaacgat ttccggcatc tgacgataga aaaaggtcaa cagcagcgta cgaatgtgcg
    18061 agccgtcgac gtccgcatcg gtcatgatga tgatgctgtg ataacgcagt ttgtccgggt
    18121 tgtactcgtc acgaccgata ccacagccaa gcgcggtgat aagcgtcgcc acttcctgag
    18181 aagagagcat cttatcgaag cgcgctttct caacgttgag gattttaccc ttcagcggca
    18241 gaatcgcctg gttcttgcgg ttacgcccct gcttcgcaga gccgcccgcg gagtcccctt
    18301 ccaccaggta cagttcggaa agcgccggat cgcgttcctg gcagtctgcc agtttgcccg
    18361 gcaggcccgc taagtcgagc gcacctttac ggcgggtcat ttcacgcgcg cgacgcgccg
    18421 cttcacgggc acgggcagca tcgataattt tgccaaccac gattttcgcg tcggttgggt
    18481 tttccagcag gtattctgcc agcagttcgt tcatctgctg ttcaaccgct gatttcacct
    18541 cagaagaaac cagtttgtct ttggtctggg aggagaattt cgggtccggc actttcacgg
    18601 aaacgaccgc aatcaggcct tcacgcgcat cgtcaccggt ggcgctaact ttggcttttt
    18661 tgctgtagcc ttctttgtcc atgtaggcgt tcagggtacg ggtcatcgcc gcacggaagc
    18721 ctgccaggtg agtaccgccg tcacgctgcg gaatgttgtt ggtaaagcag tagatgtttt
    18781 cctggaagcc atcgttccac tgcaacgcca cttcgacgcc aataccgtct ttttcagtgg
    18841 agaagtagaa gatattcggg tggatcggcg ttttgttctt gttcagatat tcaacgaacg
    18901 ccttgatgcc gccttcatag tggaagtggt cttctttgcc gtcgcgcttg tcgcgcagac
    18961 gaatggaaac gccggagttg aggaacgaca actcacgcag acgtttcgcc agaatttcat
    19021 attcgaactc ggtcacattg gtgaaggttt cgaggctggg ccagaaacgc accatggtgc
    19081 cggttttttc agtctcgccg gtaaccgcca acggtgcctg cggtacaccg tgttcgtaga
    19141 tctgacggtg aattttaccc tcgcgctgga taaccagctc cagtttttgc gacagggcgt
    19201 ttactaccga aacaccaacg ccgtgcagac cgccggacac tttataggag ttatcgtcaa
    19261 atttaccgcc tgcgtgcaga acggtcatga tcacttccgc cgccgatacg ccctcttccg
    19321 ggtgaatacc ggtcggaatg ccgcgcccgt catcctgtac agagacagag ttatcggcgt
    19381 gaatggtgac gataatttct ttacagtgac ccgcgagcgc ttcgtcgata gcgttatcta
    19441 ccacctcgaa taccatgtgg tgcagaccgg tgccgtcatc cgtgtcgccg atatacatac
    19501 ccgggcgctt acgcaccgca tccagccctt tcaggacttt gatactggag gagtcataag
    19561 aattcgacat caacgtttct cgctcattta tacttgggtt aatccgttat tttacccttt
    19621 tccacggtaa acatcttcga attttcgtcc gacatgtcta taacgtgttc agcactgatc
    19681 gcgctgacaa agacctgtga ttgcgtcgct tttaagcggc tggcaagcag cccgcgacgc
    19741 tcatcatcaa gctcagaggc aaaatcatct atcaggtaga gacaccgccg cccgctttca
    19801 cgggtgagga actctccttg cgccagacgt aaggcgcaca tcaacagctt aagctgccca
    19861 cgcgataagg tatcttccac cggcgcaccg tcggcgcgaa tgcgtaagtc cgctttatgc
    19921 gggccatgcg cggtgtaggt tagctggcga tcgcgttcaa aattacgttc cagcacctca
    19981 gcatattctg tctctttctc ccagccgcgc tggaaagaga aagtcagaga aaactcaggg
    20041 agaaattgct tacaagtatc ggccatatca gccgcgatac cggcgctata ctccgcgcgc
    20101 caggtgctga tttgctccgc cagcgggatc agctctttat cccacgggcg tagctgttcg
    20161 taacgtgtca cctggcgcag cgccgcattg cgctgcttga gcaatcgctt gagattgctc
    20221 caggcggtga aaaatccggg ttcgttgtga aagcatcccc agtcgaggaa tgctcttctg
    20281 tatttggggc cgccgttgag taaagtaaac ccttctggcg ttatcaactg cattggcatc
    20341 aggtgcgcca gttccgcgac cttatgcccg tctgtaccgt cgatgcggac tttgctgtcg
    20401 ccctgtttgt ctttggttaa gccaatcgct gtctcgcgct cttcgccctg taatcgcccg
    20461 tggagaacaa acgcctcctg ctcatggcga atgacgcgac caatctgcaa actgcgaaac
    20521 gcccgaccat ggccgagcgt atagatggct tccagcacgc tggttttgcc actgccgttg
    20581 gcacctacca gaaagttaaa gccgggagat aaagcgagat ccgcggtttc aatgttgcgg
    20641 aaatcgcgga tcaacaagcg ggtgagggac attacagtct cattggcatg acaacatagg
    20701 cagccgactg tgatgcggca tcttcaatct gcacgctgga aaccgaatcg gtcagcatca
    20761 tgcggacgtt ttcacatttc agcgcgttca gaacatccag cacgtagctg acgttgaagc
    20821 cgatttccat ctccgcaccg ctataggtaa cgtcgaggat ctcttccgct tcttcctgtt
    20881 ccgggttgtt ggcggtgatt ttcagctggt tttcgctgac ataaagacgt acgccgcgga
    20941 atttctcgtt agagagaatc gccgcgcgag caaacgcctg cttgagcaga tcgcagccag
    21001 cttccagatg tttgtccggg ttcttcggca gaacgcggcg ataatccggg aagcgaccat
    21061 ccaccagttt ggaggtgaag ataaagtcgc caacgtgggc gcgaatgttg ttgctgccaa
    21121 tctgtacgcg cagcggattg tcgccgccgt cgagcatacg catcagttca atcacgcctt
    21181 tacgcggcac gatcaccgaa tggcttggca aagattgacc aattggcatt gaacagactg
    21241 ccagacggtg gccgtcggtc gccacggtgc gcagttcttc gccttcggtt tcaaacagca
    21301 taccgtttaa gtaatagcga acgtcctgat gcgccataga aaactgggtc gcttcaatca
    21361 gacgcttcat cgtcgcctgc ggcagggtaa attcgacttc gctctgccag tcatcgaggt
    21421 tcgggaaatc cgccgctggc agggtagaca gcgaaaaacg gctacgcccg gagcgcacca
    21481 gcatccgttc accttccagc tgtacggcaa tttccgcgcc ttcaggcaga ccacggcaga
    21541 tatcaaagaa tttgcgcgcc ggaacggtcg tcgctcctgg ctcgtgtggc tgaaccagcg
    21601 caacgcgtgc caccatttcc atctcgagat cagtaccggt cagcgacaac gtaccgtcag
    21661 caacctgtaa cagcagatta ccgagaatcg gtagcgtagg acgaccacct aacggcccgc
    21721 tcacctgttg tagcggtttt aataaatgct cacgttctac ggtaaatttc ataggtttac
    21781 gatgacaatg ttctgattaa atttgaaaaa tcttctttga tatcgtggct ctcttcacgc
    21841 aactgctcga tcttacggca ggcatgaagc accgtcgtgt ggtcacgacc accaaacgca
    21901 tcgccaatct ccggcagact gtggttagtc agctctttcg ccagcgccat cgccatctgg
    21961 cgcggacgcg ccaccgagcg ggatcgacgc ttggaaagga gatccgcgac tttgatcttg
    22021 tagtactccg ccaccgtctt ctgaatattg tcgatggtga ccagtttttc ctgcaatgcc
    22081 agcaagtcgc gcagtgcctc acgcacgaag tcgatggtga tcgcccgtcc ggtaaagttg
    22141 gcgttagcaa taacgcggtt cagcgcccct tccagctcac gtacgttaga tcgtagacgc
    22201 ttggcgataa agaacgccac ttcgcctggc aaacgaatgt cattttcgtc ggcctttttc
    22261 atcaggatcg ccacgcgggt ttccagctct ggcggttcga tcgccacagt cagtccccaa
    22321 ccgaagcggg atttcaaacg atcctcaacg ccgttgatct ctttcggata gcgatccgag
    22381 gtgagaatga tctgttgatt accttccagc agggcgttga aggtgtggaa aaactcttcc
    22441 tgagatcgtt ctttattagc aaaaaactga atatcgtcga tcagcagtgc atctacggaa
    22501 cggtagtagc gtttaaactc ttcgatcgcg ttgttttgca gggctttaac catgtcctga
    22561 acaaagcgct cggagtgcat ataaaccact ttggcattcg gcttgcgcgc cataatgccg
    22621 ttacccaccg catgcagcag gtgagtttta cccagacccg tgccgccata aaggaacaat
    22681 gggttatagg caccgccagg gttatccgcc acctggcgag ccgccgcgcg cgccagttgg
    22741 ttagatttac cttcaacgaa gttatcaaac gtgtgtttga cgtttacgtt agaacgatag
    22801 gtcggttctg ccggggccgg gacgttatcc caacctgagc gcgtagaagg cgcagcacgt
    22861 tgcggctgcg tttgcgccac ctgtgcaggg gccgcgacgt tgctcgtcac tgccgcttgt
    22921 ggcgtttgcg tcaccggttt ggtgccgact tcaaaacgca gctgtggggc atccgctccg
    22981 cagaaactgg ttagcagtcc attgatatta ttaaggtact tgtcccgtac ccaatcgagg
    23041 acaaaacggt ttggcgcgta cagggccagc gtgttatcgc tcagttccgc ctgcaatggg
    23101 cgtatccaca tactgaattc tgtggctggt aactcatcct gcaatcgggc aagacactgc
    23161 tgccaaagcg aaagtgacac ggcggactcc actcgaacaa aagtcgataa tgactaaggc
    23221 tgaaacattc atgattgttg acgtacgtcg aaaagaccct gcttgagggt gacgcacgaa
    23281 ccgctgtctg cggttatatg cccgatcaag atcctgcaaa acgatcggga ccgcggatca
    23341 tagcctaaac tgcgcaagag atcttctgtt tctcacagat tttcccgatt tatccacagg
    23401 actttccaga cctcgctaag tgtaaacgat cctgccgcga ggcgggcacg atttacgccg
    23461 catattggaa aatttaatga ccatagacaa aaattggctt aatcgatcta ataaagatct
    23521 aggacgatcc ttgcgcttta cccatcagcc cgtataatcc tccacccggc gcgccatgct
    23581 ggttttcact ggtgtgaggt cgtacatttt ccctgcgaaa aggtgcggaa aagcgcggta
    23641 aataaggaaa gagaattgac tccggagtgt acaattatta caatccggcc tctttaatca
    23701 cccatggctt cggtgtccat cgtttcattt ttcggcggat atccaataaa gccattgaat
    23761 ttattcaagt ttaggtagaa atcgccatga aacgcacttt tcaaccgtct gtactgaagc
    23821 gcaaccgttc tcacggcttc cgtgctcgta tggctactaa aaatggtcgt caggttctgg
    23881 cacgtcgtcg tgctaaaggc cgcgctcgtc tgaccgtttc taagtaataa agctaacccc
    23941 tgagtggtta agctcgcatt tcccagggag ttacgcttgt taactcccag tcaattcaca
    24001 ttcgtcttcc agcagccaca acgggctggc acgccgcaaa ttaccattct cggccgcctg
    24061 aattcgctgg ggcatccccg tatcggtctt acagtcgcca agaaaaacgt tcgacgcgcc
    24121 catgaacgca atcggattaa acgtctgacg cgtgaaagct tccgtctgcg ccaacatgaa
    24181 ctcccggcta tggatttcgt ggtggtggcg aaaaaagggg ttgccgacct cgataaccgt
    24241 gctctctcgg aagcgttgga aaaattatgg cgccgccact gtcgcctggc tcgcgggtcc
    24301 tgatagccct cattcgggtc tatcaacgcc tgattagtcc gctactcggg ccgcattgtc
    24361 gtttcactcc aacctgttca agctacggaa ttgaggcatt gcgcaggttt ggagtgataa
    24421 aaggcagttg gttgacggtg aaacgcgtat taaaatgcca ccctttacac cctggtggtg
    24481 acgatcccgt cccgcccgga ccatttgata ccagagaaca ctaacgatgg attcgcaacg
    24541 caatctttta gtcatcgctt tgctgttcgt gtctttcatg atctggcaag cctgggagca
    24601 ggataaaaac ccgcaacctc aggcccaaca gaccacgcag acaacgacca ccgcagcggg
    24661 tagcgccgcc gaccagggcg taccggccag tggccagggg aaactgatct cggttaagac
    24721 cgacgtgctt gatctgacca tcaacacccg tggtggtgat gttgagcaag ctctgctgcc
    24781 tgcttacccg aaagagctga actctaccca gccgttccag ctgctggaaa cttcaccgca
    24841 gtttatttat caggcacaga gcggtctgac cggtcgtgat ggcccggata acccggctaa
    24901 cggcccgcgt ccgctgtata acgttgaaaa agacgcttat gtgctggctg aaggtcaaaa
    24961 cgaactgcag gtgccgatga cgtataccga cgcggcaggc aacacgttta ccaaaacgtt
    25021 tgtcctgaaa cgtggtgatt acgctgtcaa cgtcaactac aacgtgcaga acgctggcga
    25081 gaaaccgctg gaaatctcca cctttggtca gttgaagcaa tccatcactc tgccaccgca
    25141 tctcgatacc ggaagcagca acttcgcact gcacaccttc cgcggcgcgg cgtactccac
    25201 gcctgacgag aagtacgaga aatacaagtt cgataccatt gccgataacg aaaacctgaa
    25261 catctcttcg aaaggtggtt gggtggcaat gctgcaacag tatttcgcga cggcgtggat
    25321 cccgcataac gacggtacca acaacttcta taccgctaat ctgggtaacg gcatcgccgc
    25381 tatcggctat aaatctcagc cggtactggt tcagcctggt cagactggcg cgatgaacag
    25441 caccctgtgg gttggcccgg aaatccagga caaaatggca gctgttgctc cgcacctgga
    25501 tctgaccgtt gattacggtt ggttgtggtt catctctcag ccgctgttca aactgctgaa
    25561 atggatccat agctttgtgg gtaactgggg cttctccatt atcatcatca cctttatcgt
    25621 tcgtggcatc atgtacccgc tgaccaaagc gcagtacacc tccatggcga agatgcgtat
    25681 gctgcagccg aagattcagg caatgcgtga gcgtctgggc gatgacaaac agcgtatcag
    25741 ccaggaaatg atggcgctgt acaaagctga gaaggttaac ccgctgggcg gctgcttccc
    25801 gctgctgatc cagatgccaa tcttcctggc gttgtactac atgctgatgg gttccgttga
    25861 actgcgtcag gcaccgtttg cactgtggat ccacgacctg tcggcacagg acccgtacta
    25921 catcctgccg atcctgatgg gcgtaacgat gttcttcatt cagaagatgt cgccgaccac
    25981 agtgaccgac ccgatgcagc agaagatcat gacctttatg ccggtcatct tcaccgtgtt
    26041 cttcctgtgg ttcccgtcag gtctggtgct gtactatatc gtcagcaacc tggtaaccat
    26101 tattcagcag cagctgattt accgtggtct ggaaaaacgt ggcctgcata gccgcgagaa
    26161 gaaaaaatcc tgattcggtg agttttcgct aaaataaggg cggtcagttg accgcctttt
    26221 ttcttttcgt agggtggata agcaccgcgc atccgccaca caaagcaaca ggaacatcat
    26281 gagcgataat gacactatcg tagcccaggc cacgcctccg ggacgtggcg gcgttggcat
    26341 cctgcgcatc tccggcctaa aagcccgtga agttgccgaa accgtgctgg gtaaactgcc
    26401 taagccgcgc tacgccgatt atcttccgtt taaagacgcc gacggcagcg tgctcgatca
    26461 ggggattgcg ctatggttcc ctggcccgaa ctcgttcacc ggcgaagatg tgctggaact
    26521 gcagggtcat ggcggtccgg tgatcctcga cctgctgtta aaacgcattc tgaccattcc
    26581 cggcctgcgg attgctcgcc ctggtgagtt ttccgaacgc gcgtttctta acgataaact
    26641 cgacttagcc caggccgagg cgattgccga tcttatcgac gccagttcgg aacaggcggc
    26701 ccgttcggca cttaactcgc tgcaaggcgc attctccgca cgggtcaacc atctggtgga
    26761 agccctcacc cacttgcgca tttacgtcga agcggcaatt gatttccctg atgaagagat
    26821 cgatttcctc tccgacggca aaattgaagc ccagcttaat gacgtgattg ccgatctcga
    26881 tgcagtgcgt gctgaagcac gtcagggtag tttgttgcgc gaagggatga aggtggtgat
    26941 tgccggacgt cctaacgccg gtaaatcgag cctgttaaac gcgctggcgg gccgtgaagc
    27001 agcaatcgta actgatatcg ccggaaccac acgtgacgtg ctgcgtgagc atatccacat
    27061 tgacggaatg ccgctgcata tcatcgatac cgctgggcta cgtgaagcca gtgacgaagt
    27121 ggaacgtatt ggtatcgagc gcgcgtggca ggaaattgaa caggccgacc gcgtgctgtt
    27181 tatggtcgat ggcaccacaa cagacgccgt ggatccggca gagatctggc cggaatttat
    27241 cgcccgtctg ccagcgaaac taccgatcac cgtggtgcgc aataaagccg atatcaccgg
    27301 cgaaacgctg ggaatgagtg aagtgaacgg tcacgcgtta attcgtctct cggcaaggac
    27361 aggtgaaggc gtggaggtgc tgcgtaacca tctcaaacag agcatgggct ttgacaccaa
    27421 catggaaggc ggcttcctgg cgcgtcgtcg ccacctacag gcgctggaac aggcagcgga
    27481 gcatttgcag caaggtaaag cgcaactgtt aggtgcctgg gcgggtgaac tgctggcgga
    27541 agagttgcgc ctggcacagc agaacttaag cgaaatcacc ggggaattta cctctgatga
    27601 tttgctgggg cggattttct ccagcttctg tattggtaag taaccgcgct tacgaagccg
    27661 tattctgact gtcagatgcg gcttcgcttc attgttagca ctcctgttat tcctcaaccc
    27721 tttttttaaa cattaaaatt cttacgtaat ttataatctt taaaaaaagc atttaatatt
    27781 gctccc
//
LOCUS       DAZPAC010000039        27102 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZPAC010000039 DAZPAC010000000
VERSION     DAZPAC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27102
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.039"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..921
                     /locus_tag="ACHUPM_001343"
     CDS             <1..921
                     /locus_tag="ACHUPM_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0869170.1"
                     /translation="SATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLN
                     DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVN
                     IGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLT
                     APQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQM
                     RIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTV
                     NMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="ACHUPM_001344"
     CDS             complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="ACHUPM_001344"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR0869171.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1888..3924
                     /gene="lpoA"
                     /locus_tag="ACHUPM_001345"
     CDS             1888..3924
                     /gene="lpoA"
                     /locus_tag="ACHUPM_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR0869172.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3882..4277
                     /locus_tag="ACHUPM_001346"
     CDS             3882..4277
                     /locus_tag="ACHUPM_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR0869173.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4297..4887
                     /gene="diaA"
                     /locus_tag="ACHUPM_001347"
     CDS             4297..4887
                     /gene="diaA"
                     /locus_tag="ACHUPM_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR0869174.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4897..5472
                     /gene="dolP"
                     /locus_tag="ACHUPM_001348"
     CDS             4897..5472
                     /gene="dolP"
                     /locus_tag="ACHUPM_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR0869175.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5586..6626)
                     /locus_tag="ACHUPM_001349"
     CDS             complement(5586..6626)
                     /locus_tag="ACHUPM_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0869176.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6699..7334)
                     /locus_tag="ACHUPM_001350"
     CDS             complement(6699..7334)
                     /locus_tag="ACHUPM_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR0869177.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7462..7980
                     /gene="yhbO"
                     /locus_tag="ACHUPM_001351"
     CDS             7462..7980
                     /gene="yhbO"
                     /locus_tag="ACHUPM_001351"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR0869178.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7960..8403)
                     /locus_tag="ACHUPM_001352"
     CDS             complement(7960..8403)
                     /locus_tag="ACHUPM_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR0869179.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8454..8756
                     /gene="yhbQ"
                     /locus_tag="ACHUPM_001353"
     CDS             8454..8756
                     /gene="yhbQ"
                     /locus_tag="ACHUPM_001353"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR0869180.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8743..9246)
                     /locus_tag="ACHUPM_001354"
     CDS             complement(8743..9246)
                     /locus_tag="ACHUPM_001354"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0869181.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9240..9764)
                     /locus_tag="ACHUPM_001355"
     CDS             complement(9240..9764)
                     /locus_tag="ACHUPM_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR0869182.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9973..10968
                     /locus_tag="ACHUPM_001356"
     CDS             9973..10968
                     /locus_tag="ACHUPM_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR0869183.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10977..11855
                     /locus_tag="ACHUPM_001357"
     CDS             10977..11855
                     /locus_tag="ACHUPM_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR0869184.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12061..13068
                     /locus_tag="ACHUPM_001358"
     CDS             12061..13068
                     /locus_tag="ACHUPM_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFR0869185.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="ACHUPM_001359"
     CDS             complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="ACHUPM_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR0869186.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="ACHUPM_001360"
     CDS             complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="ACHUPM_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR0869187.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="ACHUPM_001361"
     CDS             complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="ACHUPM_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR0869188.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="ACHUPM_001362"
     CDS             complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="ACHUPM_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR0869189.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="ACHUPM_001363"
     CDS             complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="ACHUPM_001363"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR0869190.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="ACHUPM_001364"
     CDS             complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="ACHUPM_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR0869191.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20446..21390)
                     /gene="truB"
                     /locus_tag="ACHUPM_001365"
     CDS             complement(20446..21390)
                     /gene="truB"
                     /locus_tag="ACHUPM_001365"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR0869192.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="ACHUPM_001366"
     CDS             complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="ACHUPM_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR0869193.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21955..24627)
                     /gene="infB"
                     /locus_tag="ACHUPM_001367"
     CDS             complement(21955..24627)
                     /gene="infB"
                     /locus_tag="ACHUPM_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR0869194.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="ACHUPM_001368"
     CDS             complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="ACHUPM_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR0869195.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="ACHUPM_001369"
     CDS             complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="ACHUPM_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR0869196.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26826..26902)
                     /locus_tag="ACHUPM_001370"
     tRNA            complement(26826..26902)
                     /locus_tag="ACHUPM_001370"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26866..26868),aa:Met,seq:cat)
BASE COUNT         6419 a         7516 c         6811 g         6356 t
ORIGIN      
        1 tctgcaacgc taagtagcac taactggcaa tacgcctgtt cctgctctgc gggtaaggca
       61 gttaaacttg tctatatggt cagccccgta cttaccacca ctggacatca gacaggatat
      121 tacaaactca atgacagcct ggatattaaa accacattac aggcaaacga cattccagga
      181 ctcacaaccg accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc
      241 gtatattctg ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc
      301 gttaatattg gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg
      361 atgaccattc cgaaaacgga tattgccgtc attaaaggcg cgtgggtcga tggaatggga
      421 agcccgtcta caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc
      481 gccccacagt cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc
      541 aatggtcaga agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt
      601 gatatcactt atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc
      661 gacggtacaa ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac
      721 aatgtgcccg atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct
      781 tttcagaacg gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc
      841 tggccagtta atctggtcgg tggtgagctg gaaacaggaa aatttcaggg caccgccacc
      901 attaccgtca tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca
      961 ctgttacccc tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat
     1021 ttctgcggcc agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt
     1081 gcgcagggca tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac
     1141 catttcgcct ttgcgacggt tttcatcttc ctttacccac gccagcagct cgccaacggg
     1201 cgcgccgtga atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga
     1261 ttcgcctaat accgcaacga tatcttccag gctatctaac agacggtggg tagattcata
     1321 aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga
     1381 tttggcaggt aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa
     1441 cgcagtgata gcagcacacg gtccgggtag cggcaccaca cgaatccccg cttcacggca
     1501 ggtacgcacc agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag
     1561 cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt
     1621 atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt
     1681 gtgacgagta tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg
     1741 ggtgatatcc gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt
     1801 atccgccgat tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt
     1861 gcgtaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc
     1921 gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact
     1981 cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt
     2041 cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt
     2101 gcactggtga aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa
     2161 gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg
     2221 cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa
     2281 caaaaccagc aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc
     2341 attgatttac tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag
     2401 cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg
     2461 ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg
     2521 tttgataacc gtaacgatcc cgacatgatg aaagccggga tcgccgactg gcaaaaacgt
     2581 tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt
     2641 aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta
     2701 tttggtcgca ccattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg
     2761 gcggctcagg tagctgccgc acctgccgca gacgtagctg aacaacctca gccgcaaacc
     2821 gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgatcagcct
     2881 gcggcccagc cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc
     2941 gcaaatccat ccgcagagct gaaaatctac gatacctcat cacaaccact tagccagatc
     3001 ttaagccagg ttcagcagga tggcgcgagt attgtggttg gtccgttgct gaaaaataac
     3061 gttgaagagt tgctgaagag caacaccccg ctgaacgtgc tggcactgaa ccagccagag
     3121 aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt cacctgaaga cgaagcgcgc
     3181 gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc
     3241 agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa actgggcggc
     3301 ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc
     3361 ggttctggta tcgctttaac gggtaccccg attactccca gagcgacaac cgactccggc
     3421 atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat
     3481 ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa
     3541 ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa tgctgtacgc cagctcccgc
     3601 agcgcacaag gcaccgcagg cccggatttc cgtctggaga tggaaggttt gcagtacagc
     3661 gaaatcccga tgctggcggg cggtaatctg ccgttaatgc agcaggcact cagcgcggtg
     3721 aataacgatt attcactggc tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca
     3781 aatcatttct cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc
     3841 ctgacggcaa acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa
     3901 ggtcaggtag tccccgtcag ttaaccacca aacagaccgg cgatgcgtgg gaagcacaag
     3961 cgcgtcgctg gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg aacgagcgtg
     4021 gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct
     4081 atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca
     4141 aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg
     4201 attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct
     4261 ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc
     4321 ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc
     4381 cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt
     4441 aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa
     4501 acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg
     4561 attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat
     4621 gcgggagatg tattgttagc catttccacc cgtggcaaca gccgcgatat tgttaaagca
     4681 gttgaagccg ccgttacgcg tgatatgacc attgtggcat tgaccggcta tgacggcggc
     4741 gagcttgcag gtttgttagg gccacaggat gtggagatcc gcattccttc gcatcgtagt
     4801 gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac
     4861 acgcttttcc ctcaccagga tgattaagga gaatacatga aggcattatc gccaatcgca
     4921 gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct
     4981 gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt
     5041 accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaaacc
     5101 cgtattaatg tcacggcgta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct
     5161 gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat
     5221 aacgagattc gtcagggcca gccgattggt ctgggcgaag catctaacga tacgtggatc
     5281 accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa
     5341 gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa
     5401 gcggcggcag atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg
     5461 tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     5521 tacaacgatg caaaccgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc
     5581 tcaaatcaga ataacagcgc cagcccgccg acaatcacgc ctgacactgc caccatcgcc
     5641 cccgtcagcc ataaggcttt cgccgggaac gctttgcgca gcataatcag tgacggcaaa
     5701 ctcactgccg ggagcgtcat caacaatgcc agcgccggag cggttcccat acctgccagc
     5761 atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct
     5821 accgccatcg ccaccaccca catcaggctg ttatcgacag caccatcggc atgggggaat
     5881 aaccagacgc gtgcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc
     5941 cagaaaagtg tccatagcgc cctgccccag cggctaaaaa aaccgctctg tgcttccggt
     6001 atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc
     6061 gtcgcaatca gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag
     6121 ccgaggacaa accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag
     6181 aatgccagcg caccgcccat cgatacctgt tggcgacgca ttcccgccgc gaccggagcc
     6241 gcacagcagg tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga
     6301 aagcgcgatt gtccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac
     6361 gaaccgagga tcacccccag caccgccgct ttccataccg cgaggaaata gatcatcgcg
     6421 taatccaacg ccgcctgcca cggattagca tccgcctgcg caaggataga tttaccgata
     6481 ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag gttgccattt cacataccag
     6541 agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc
     6601 gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc
     6661 gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat
     6721 tctgaagagc ttaaaatagt cacgccttca tgctcaggcc gcatcgcctc tgccagcatg
     6781 acccgcgcca cgtctctcgc atcaatggat ttccagttac cgggcagtaa acgaaacagc
     6841 ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cgcccagcaa catcgacggg
     6901 cgagcaatgg tcaatttcgg ccagttctgg gcaattaatg cttcttccat ctcccctttg
     6961 acgcggttat agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata
     7021 tgctgcgcgc ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca
     7081 tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca
     7141 atgtcgatgg gttcggtgac ctgcgccaac gcgtcactca gttgcggatc atggggatta
     7201 aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cggctatggc gttaactttc
     7261 ggttcgttaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc
     7321 agtacctgac tcatcttcgc tcctttacag aattgtctgc cttgcgctcc acggctcatg
     7381 caccaggctt aatagaccat gaggtaatta tccccgattg tgtgaaattc gcctcatcca
     7441 atgcaacaac gcggaggaag catgagtaag aaaattgccg ttttaatcag tgatgaattt
     7501 gaggattcag aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt
     7561 accattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc
     7621 atcgataaat ccatcgatga agtgacgcca gcggagtttg atgccctgct gctaccgggt
     7681 ggtcattcac cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt
     7741 gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc
     7801 gatgtgattc gggggcgcaa actgaccgca gttaaaccga tcattattga tgttaaaaat
     7861 gcgggcgcgg aattttacga tcaggaagtc gtggttgata aagatcagct ggttaccagc
     7921 cggacaccgg acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga
     7981 gtcgcgtagc caaatcattt ttttaccaaa gcccagcgtg ttgtcggtga atttgatttc
     8041 atcgagccgg atttcccaca ccggggccga cagcattctg gcaaccggaa agcgacgatt
     8101 gtacgcctgg cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa
     8161 ctgcacgccg cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc
     8221 agcctgcggc ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc
     8281 gactttctgc gcatcaaaca gataaaaagc attagcgcac cacagttccc cttcctgctg
     8341 cacacaccag gtgacaacat gttgttttgc caaccaacgg ctgatggtga tgagtgtttc
     8401 cattgctgct ctcttttata ctgtgggcct gaccttaaca tgcgacgcag acgatgacac
     8461 cctggtttct ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg
     8521 atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga
     8581 aaggagaact gacgctggcg ttttctgcgc cagtgggcga tcgttcgctg gcgttacggg
     8641 cggaatatcg cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg
     8701 cagggtttgc agagctgtta agcagtctgc aaaccccgga gattaaaagc gattgaaatg
     8761 ctcgtgatac tcaaccagac cggttacgcc attcagcgcg tcatccgcta aacgatgtac
     8821 ctggaaggcg ctttcagtgc ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc
     8881 aaagccgaaa cgactgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa
     8941 ctcattaagc gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta
     9001 tttttcatca accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc
     9061 aaccggacta aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc
     9121 cagcgtcaga aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc
     9181 gaatgagcga cgcagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat
     9241 tagcatggtt cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca
     9301 acaaaatccg ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca
     9361 atggcgtcca tcaggttttt cacatacagc cccagctccg tatcgccttc aatcaccagc
     9421 cgacgctgga agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg
     9481 ctggcgtcgg cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca
     9541 ttcaccaccg aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg
     9601 ccttcaagaa actccagctc accatcatcc agcgcctgac ggaattgcca gcttaagacc
     9661 tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga
     9721 gatggcccca aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt
     9781 ttcaacaatc atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat
     9841 tccatatgaa attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc
     9901 gcagcaaggt taactaaaat cccagttcgt taacattttt gcgttttgaa agcgcaacct
     9961 tcaggaaaaa ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc
    10021 atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag atgatactaa tgcccgtcac
    10081 ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa
    10141 catcgccgca aacttcacat cgcgattaac acttttgcgc atccggacgg ttacgcccgt
    10201 tggcagcgcg ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac
    10261 ctcgccatgc tggagtacgc cgccgagcgt tacccgcata ttgagcgtca tgtgtcagtg
    10321 caggcttcgg cgaccaatga agaggcgatt aacttttatc atcgccattt tgatgttgcc
    10381 cgcgtggtgc tgccgcgcgt gttgtcgatt catcaggtga aacagctggc acgggtcaca
    10441 cctgtcccgc tggaagtctt tgcttttggc agcctgtgca ttatgtcgga aggtcgttgc
    10501 tatctgtcgt cgtatctgac gggtgagtcg cccaacaccg tgggcgcgtg ttctccggcc
    10561 cgtttcgtgc gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg
    10621 atcgaccgtt atcaggatgg cgaaaacgca ggttatccga cgctgtgtaa agggcgttat
    10681 ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa
    10741 ctgctgccgg agttaatggc ggcgaatatt gcttcggtga aaattgaagg ccgccagcgt
    10801 agcccggcgt atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag
    10861 gccgatccgc aaaacttcgt accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc
    10921 gaaggcacgc agaccaccct tggcgcgtat caccgtaaat ggcagtgaga agagcaatga
    10981 aatattcctt agggccagtg ctgtggtact ggccaaaaga ggcgctggaa gaattttatc
    11041 agcaggccgc caccagcagc gccgacgtga tttatcttgg tgaagcggta tgcagcaagc
    11101 gccgggcaac caaagttggc gactggctgg agatggcaaa atcgctcgcc ggaagcggga
    11161 agcagatagt gctctccacg ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga
    11221 aacgctatgt tgagaacggt gagtttctga ttgaagccag cgatctcggc gtggtgaata
    11281 tgtgcgctga acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg
    11341 tgacgctgaa aatattgctc aaacagggca tgatgcgctg gtgtatgccg gtggagcttt
    11401 cccgcgactg gctggtgaat ctgcttaatc agtgcgatga gttggggatt cgcaaccagt
    11461 ttgaagtgga agtcctgagc tacggtcatc taccgctggc ctactccgcc cgctgcttta
    11521 ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga
    11581 acgggcgcaa cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat ggcattcaga
    11641 ccatgagcgg ctacgtttac aacctcggta acgagctgac atccatgcag ggattggttg
    11701 atgtggtccg cctgtcaccg cagggtactg acactttcgc gatgctcgac gccttccgcg
    11761 ctaatgaaaa tggcgctgcg ccactgccgt tgacggcaaa cagcgactgt aacggctact
    11821 ggcggcggct ggctgggctg gagctgcaag cctaagtaaa tatgctcgtc atacttcaag
    11881 ttgcatgtgc tgcatctgca ttcgttcacc ccagtcactt acttatgtaa gctcctgggg
    11941 attcactctc ttgtcgcctt cctgcaactc gaattattta gagcacagct cactttgtta
    12001 acaactttaa ctactcttta atgcagtatt aaagattaat cggtaacaaa gtgagctgtt
    12061 atgactgata aaaccattgc gttttcgcta ctcgatctgg cccccattcc cgaaggttct
    12121 tcagcgcgag aagcattctc ccactctctc gatctcgccc gtctggctga aaagcgcggc
    12181 tatcatcgct actggctggc agaacaccac aatatgactg gcattgccag tgctgccacg
    12241 tcggtattga ttggctatct ggcggcgaat accaccacgc tgcatctggg gtctggcggc
    12301 gtgatgttgc ctaaccactc accgttggtc attgccgaac agttcggcac gctcaataca
    12361 ctctatccgg ggcgaatcga tttggggctg ggtcgtgcgc caggtagtga ccaacggaca
    12421 atgatggcgc tgcgtcgcca tatgagcggc gatattgata atttcccccg cgatgtcgcg
    12481 gagctggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc
    12541 tatggcgaga aaatccccgt gtggttgtta ggctccagcc tttacagcgc gcaactggcg
    12601 gcgcagcttg gtctgccgtt cgcgtttgcc tcacacttcg cgccggatat gttgttccag
    12661 gcgctgcatc tttatcgcag caacttcaaa ccgtcggcac gactggaaaa accatacgcg
    12721 atggtgtgca tcaatattat cgccgccgac agcaaccgcg acgctgaatt tctatttacc
    12781 tcaatgcagc aagcctttgt gaagctgcgc cgtggcgaaa ccggacaact gccgccgccg
    12841 attcaaaata tggatcagtt ctggtcaccg tctgagcagt atggcgtgca gcaggcgctg
    12901 agtatgtcgt tggtgggtga taaagcgaaa gtgcgtcacg gcttgcagtc gatcctgcgc
    12961 gaaaccgacg ccgatgaaat tatggttaac gggcagattt tcgaccacca ggcgcgcctg
    13021 cattcgtttg aactggcgat ggatgtgaag gaagagttgt tgggatagtg tgtcttaacg
    13081 cgggaagcct tatccgagct ggcaacgctg tcctacgtag acctgataag cgaagcgcat
    13141 caggcattgt gtaggcagca gaaatgtcgg ataaggcacc gctgattact gatacaccgg
    13201 cagcaaatta aagctcgata agatatggac cagtgcgttg ccaacgccaa acaccagaat
    13261 cagcgtaatc atcggcttgc cgccccagac acggaatttc gggctgccaa agcgtttacg
    13321 cgatgcgcgg gctaacagcg ccggaacaat tgccgcccag atggtagctg ctaaaccagc
    13381 ataaccaatg gcgtacagga atccgttcgg gaacagtaga ccacccacaa caggcggggc
    13441 aaaggtcagc aatgccgttt tcaagcgccc cagggctgag tcatcgaaac caaacagatc
    13501 tgccagatag tcaaacaaac ccagcgttac gccaaggaac gaactcgcta ccgcaaagtt
    13561 tgagaacacg accagcagaa ggtccaggct acggctgttc agcacgccgc ttaatgcctg
    13621 taccagcaca tcaatattac cgcccttctc tgcaataccg ataaactccg gacgcgggat
    13681 gttacccatc gtcgccagca accagatggt atacagcgcc agtgccatca gcgtaccgta
    13741 caccaggcat ttcacgatgg ttttcggatc tttgccgtaa tacttcatca ggctcggcac
    13801 gttaccgtga taaccaaacg atgccagaca gaacggcagg gtcatcagca aatacggcgc
    13861 ataagatgca ttgctttcgg cgacgttgaa caatgtcgca ggctgcacat gccccagtag
    13921 gctaccgaag gtgaggaaga aggtaatgac tttcgccccc agtacaatcg ccgtcatgcg
    13981 gctgacggcc ttagtactca accacaccac aaacgctacc agcagtgcaa aaccaaaacc
    14041 cgccgcccgt gccgggacgt ttagcgacat ctctgcgaag gtgtgatgca gaattgaacc
    14101 actggcagaa atataggcat aggtcaggat atagagcaca aaggcaatgg aaataccgtt
    14161 gaccacgttc cagcctttgc ccagcaaatc tttggtgatg gtgtcaaaac tcgaaccgat
    14221 tcggtaattc aggttagctt ccagaatcat caagccggaa tgcagcatac agaaccaggt
    14281 aaagatcagc gccgccattg accagaaaaa ccacgccccg gacatgacca ctggcagaga
    14341 aaacatccct gcgccaataa tggtgccgcc gataatcacc acgccgccaa gcagcgacgg
    14401 tgacgtttgg gtggtggtta gtgttgccat gagagcttct ctccagtgaa aaatagtgcg
    14461 actgcgttgt tatgcattgc actgtaccag tacacgagta caaaagacag aaaaaaagcc
    14521 ccgatggtaa aaatcggggc tgtatatatt attttacaga ttgtgttcgc tgttcagcga
    14581 tgattacgca tcaccaccga aacgacgacg accggtagag tcatcacgac gcggagcgcg
    14641 gccttcacga cgttcgccgc taaaacgacg accatcacca cggccacctt cacggcgttc
    14701 accgctgaag ttacgaccgc cttcacgacg ttcgccaccg aaaccacgac caccgccacg
    14761 acgctcaccg ccagtatgcg gctgtgcatc gcccagtaac tgcatgttca tcggcttgtt
    14821 gagaatgcga gtgcgcgtaa agtgttgcag cacttcaccc ggcatacctt tcggcagttc
    14881 gatggtggag tgagaagcaa acagcttgat gttaccaatg taacggctac tgatgtcgcc
    14941 ttcgttagca atcgcaccaa cgatatgacg aacttcaaca ccatcatcgc ggcccacttc
    15001 aatgcggtac agctgcatat cgccaacatc acgacgttca cgacgcggac gatcttcacg
    15061 gtcaccacgc gggccacggt cgttacgatc gcgcggacca cggtcatcac ggtcacggaa
    15121 ttcacgtttc ggacgcatcg gcgcatctgg cggtacgatc agagtacgtt caccctgtgc
    15181 cattttcagc agtgccgcag ccagagtttc gagatccagc tcttcacctt ctgcagtcgg
    15241 ctgaattttg ctcagcagtg cgcggtattg atccagatcg ctgctttcca gctgctgctg
    15301 tactttagcg gcgaattttt ccagacggcg tttgcccagc agttctgcgt tcggcagttc
    15361 tacttccgga atagtcagct tcatagtacg ttcaatgttg cgcagcagac gacgctcgcg
    15421 gttctcaacg aacagcagcg cgcggccagc acgacccgca cgaccggtac gaccgatacg
    15481 gtgaacgtaa gactcagaat ccatcgggat atcgtagtta actaccaggc tgatacgctc
    15541 aacgtccagg ccacgggctg caacgtcggt cgcaatcagg atgtccagac gaccatcttt
    15601 caggcgttcc agtgtctgtt cacgcagcgc ctggttcatg tcaccgttca gcgcggcgct
    15661 gttgtagccg ttacgctcaa gagcttcagc cacttccaga gtcgcgtttt tggtacgaac
    15721 gaagataatc gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg cttcgttttt
    15781 gcgcataccc cagacagtcc agtagctctg gctgatgtca ggacgggtag tcacgctgga
    15841 ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg gtaatgcgac gaatcgcttc
    15901 cggcatggtt gcagagaaca gagcggtctg atgaccttcc gggatctgcg ccataatggt
    15961 ttcaacgtct tcgatgaagc ccatgcgcag catttcgtca gcttcatcca gaaccagacc
    16021 gctcagttta gagaggtcca gagtgccacg tttcaggtgg tccagcagac ggcccggagt
    16081 accgacaacg atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat aacgctggcc
    16141 gccgtacaga gcaaccacat ttacgccgcg catgtgttta gagaaatccg tcattgcttc
    16201 agcaacctgt accgccagtt cgcgggtcgg tgccagcacc agaatctgtg gtgctttcag
    16261 ctcaggatca agattctgca acagaggtaa agagaatgct gcagtttttc cgctccccgt
    16321 ctgggccata cccagaacgt cgcggccatt cagcagatgt ggaatacact ctgcctgaat
    16381 tggagatggt ttttcgtaac ccagatcgtt aagggcttca aggataggag ccttcaggcc
    16441 cagatctgca aaagtggttt cgaattcagc catgtagtac gtgtgcctca aaattaatgg
    16501 cggccagtct acataactca tcatgaaatt gatcagcaat tttcattgaa aagtgtgaac
    16561 cggctcaaag taggtgtatt aacgaacaac aacgccctca cccgttaagg tgatggcaat
    16621 caaaaaagat tacgggctga tgtgtacgtc agctattgct ggtccgattc tgccaggtca
    16681 tcttggtcct ggcccaggag cgataattcc aacaatgcgt atcggtgctc aacaaagtta
    16741 tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa atcccccaga
    16801 cttaggtagt acttacctaa atagaagttg gtttcactga gatgctcagc gagcgaggtg
    16861 ttatccgttg cgtccgcctt gagcctttcc attaacgttt gttcgctaat gttgcccaag
    16921 tagaactcga caatgttcca tccccactgt tccttatccg atttttcgaa gtgctgtttc
    16981 aacacttctt tagcctgctt ctcatcgagc ttctgctcgg cgagataaag ccacagacta
    17041 cggaaaggat cattgggatc gtcttgataa aacgccagca gatcatcttg cgctaacttg
    17101 tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt gtaagttgga
    17161 tcaagctcaa gtacagaatc aaacgcttca taggcagcat caaaattgcc tgcctgcgtt
    17221 aaatatatgc ctaagtaatt gaatacttca ggcatatccg gtcggattgc cagcgcttgc
    17281 gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat catacaacac tccgcgctca
    17341 tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat ttgttccata
    17401 cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag gacttcactt
    17461 ttacgccagg aagtattact gcatcctgca agcgtaagtg ctgtcgcaac gaaacaccag
    17521 cgcaaaaaag gcttcatttc ccactcccga agaccacggt tgaatgaacg tcctgttccc
    17581 ggttgctaac aaggcgtcct gcccggttaa aagccccccg ccgcagcgga gggcaaatgg
    17641 caaccttact cgccctgttc agcagccgga gcttccggtg ctgcagcagg ttgagactgc
    17701 tcagtcgctt ctttaatgct cagacggata cggccctggc gatcaacttc tagaactttc
    17761 accggtactt cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg tttgtcagcg
    17821 atttgagaga tgtggaccag accttcttta ccgccgccga tggcaacaaa tgcgccaaag
    17881 tcaacgatac gggtcacttt accagtgtag acgcggccca cttcgatttc tgcagtgatc
    17941 tcttcgatac gacgaatagc atgtttcgct ttctcgccgt cggtcgctgc gatcttcaca
    18001 gtaccgtcat cttcgatttc gatggtagtg ccagtttctt cggtcagggc acggataaca
    18061 gagccgcctt taccgataac gtctttgatc ttatccgggt tgatcttgat ggtatggata
    18121 cgcggtgcga actcagagat atcgccacgc ggcgcgttga tcgcctgttc cattacgccc
    18181 aggatatgca gacgcgcacc tttagcctgg ttcagcgcaa cctgcatgat ctctttggtg
    18241 ataccttcaa ttttgatatc catctgcagt gcagagatac cgtcgcggga acctgcaact
    18301 ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag tacaacgtag
    18361 ttgtcgcctt ctttcaccag acccattgcg atacccgcaa cggcagcttt gattggcaca
    18421 cctgcgtcca tcagcgccag agacgcgccg cacacggaag ccatagaaga agaaccgttg
    18481 gattcggtga tttcagacac tacacgtacg gtgtacggga atttgtccat atccggcatg
    18541 actgccagca cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg cttcggagaa
    18601 ccgaccatgc cggtttcgcc tacggagtac ggagggaagt tgtagtggaa caggaaggta
    18661 tcggtacgtt cgcccatcag ttcatcaaga acctgcgcgt cacgagcggt acccagcgtt
    18721 gcagtaacca gtgcctgcgt ttcaccgcgg gtgaacagcg cagaaccgtg agtacgcggc
    18781 agcacgccag tacgcacatc cagaccacgg atcatatctt tttcacgacc gtcgatacgc
    18841 ggttcgcctg ccagtacgcg gctacgaaca acgtttttct cgatagcgtg cagaatttca
    18901 cccagttcgt tttcgtccag ggtttcgtct tcagcaagca gcgtcgcgat ggtttcagat
    18961 ttgatgacat caacctgcgc ataacgctct tgtttgtcgg tgatgcggta agcatcactc
    19021 agacgagctt cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac cggctccggc
    19081 tgccagtccc aacgcggttt accggcttct ttcactagtt cattgatgtt ctgaataaca
    19141 acctgctgtt gttcatgacc gaacactact gcgcccagca tctgatcttc gctcagcagt
    19201 tcagcttcag attcaaccat cagtacagcg gcttcagtac cggcaacaac cagatccagt
    19261 ttgctctctt tcagctcgtc ctgagtcggg ttcagtacgt actggtcatt gatgtaacct
    19321 acgcgggcag caccaatcgg gccattgaac ggaataccag acagagacag tgctgcggaa
    19381 gcaccaatca tcgcgacgat atccgggtta acctgcgggt taacagaaac cacggtggcg
    19441 ataacctgaa cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg gcggtcaatc
    19501 agacgcgcga tcagggtttc gccttcgctt ggacgacctt cacgacggaa gaagctaccc
    19561 gggatacgac cagcagcgta ggtacgctcc tgatagttaa cggtcagtgg gaagaagtcc
    19621 tgacctggtt tggctttttt ctgtccaaca acggtaacga ataccgcggt gtcatccatg
    19681 ctaaccataa cagcggcagt agcctgacga gccatcatgc cggtttccag agtcacggtg
    19741 tgttggccgt actggaattt acgaacgatc ggattaagca atgtaatatc ctttctcttt
    19801 cttagacagt accttacggc actggtgtta atacccgatc ttctgcgcat cctcgcgact
    19861 aatgacaacc ctaacccagc tctatgtggg taaagcctct cattagccgc gcgaacctct
    19921 gcaacggaag atcattcata gcaacaatac attagtttcc agtgaattgc tgccgtcagc
    19981 ttgaaaaaag gggccactca ggcccccttt tctgaaactc gcaagaatta gcgacgcaga
    20041 cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc
    20101 agtttacgac gctgagaaac catgcgcagc agaccacgac ggctgtggtg atcttttttg
    20161 tgctctgcaa agtggccctg caggtggttg atctgtgcag tcagcagtgc tacctgaact
    20221 tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt agctgttgct
    20281 tcagtactta gagacatttt aaaactccaa agtatataga atgaaaggac gccgatctct
    20341 aattcagcga tcccagtgta cgttacgcaa agtgttaaac aatttacgcg acgttaagcg
    20401 gcagtattct actcgtagcg acctgttatc gcaagacggt taacattacg ccgggtattc
    20461 aaccaccagg cgacgaggcg cgacgcggcc ttcatcgtca atttcgccca taccgataaa
    20521 cttgccgttc tcaccttctg taacgcgaac cagtccttcc agcggcgcac cggatgtacg
    20581 aactgggtta ccatttttga agtaaacaga agacgttaac ggaagattca ccaccgggta
    20641 gtccgaagct ggactgtcca ttggcatcag taatggatca agtaactccg cagccggaat
    20701 atcctgctgt tcggcttgtt cgaccagttc acgcagatgt tccagggtca ccatccgttc
    20761 aaccggatat ttacttaccg ccagacgacg caggtaaata acatgcgcgc cacaaccgag
    20821 tttttcaccc aggtcatcaa tgatggtgcg gatataagtg ccttttgagc agtgaatttc
    20881 cagctccagc tcattgcctt cgtggcgaat aaacagcaat tcataaacgg taatcgggcg
    20941 cgcttcacgc ggtacttcaa tgccctgacg cgcatattcg tacagttttt taccctgata
    21001 tttcagtgct gaatacatcg aagggatctg ttcgatatcg ccacggaaag tatccagcgc
    21061 cgccgccagt tgctctgcgc taaaggttac cggacgttct tcaacgattt gtccgtcggc
    21121 atcagaagta tcggtacgct gtccaagacg cgcaatgacc cgatagcgtt tgtcggagtc
    21181 cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa attggcaaca tgcctgttgc
    21241 cagcgggtcc agcgcaccgg tatgcccggc acggttggcg ttataaatcc gtttcacttt
    21301 ttgcagcgca tcattgctgg acataccctg cggtttatcc agcaacaaaa cgccgttaat
    21361 gtcgcgaccg cgacgacgag gacgactcat tagtcctcct tgctgtcgtc cgggttaaca
    21421 cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg catcccttca
    21481 accagagagt tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg catcgctttc
    21541 cccagcaggc tgcggatgaa accagaagct tcttgcaacg ctttgatgcc cgctttaacc
    21601 gcgtcttcat ctttgtcgtt gaggaacgtc acatatactt tggcatacgc caggtcgcga
    21661 gacatttcga caccggaaac ggtggtcatc atgcccaggc gaggatcttt aatttcacgc
    21721 tgcaggatga gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat
    21781 tctttcgcca taataaattc tcctgacaaa aaaggggctg ttagcccctt tttaaaatta
    21841 atttcaggtg gaagggctgt tcacgttggc ctgataagac gcgccagcgt cacatcaggc
    21901 aatccatgcc ggatgcggcg taaacgcctt atcccgcatg gaaccctaaa aaccttaagc
    21961 gatggtacgt tggatctcga tgatttcgaa tacttcgatc acatcgccag tgcggacgtc
    22021 gttgtagttc ttaacgccga taccacattc cataccgtta cggacttcgt taacgtcatc
    22081 tttgaagcgg cgcagggact ccagctcgcc ttcgtagata accacgttgt cacgcagaac
    22141 gcggatcggg ttgtgacgtt taaccacacc ttcggtaacc atacagcctg cgatagcacc
    22201 aaatttcggc gatttgaaca cgtcacgaac ttccgccaga ccgataatct gctgtttcag
    22261 ttccggagac agcataccgc tcatcgccgc tttcacttcg tcaatcaggt tatagatgac
    22321 ggagtagtaa cgcagatcca ggctttccgc ttcaatcact ttacgtgcag aggcatcagc
    22381 acgtacgttg aagccaacca ggatggcgtt ggacgccgca gccagggtgg cgtcggtttc
    22441 ggtgatacca cctacgccag aaccgatgat cttcacttta acttcgtcag tagacagttt
    22501 cagcaaggag tcggagatcg cttccacaga accctgtacg tctgccttca ggacgatgtt
    22561 cacttcgtga acttcgcctt cggtcatgtt ggcgaacatg ttctcgagtt tagatttctg
    22621 ctgacgcgcc agtttaactt cgcggaattt accctgacga tacagtgcaa cttcacgcgc
    22681 tttcttctcg tcacgtacaa cggtaacttc atcacccgca gccggtacgc cggacaggcc
    22741 gaggatttcc accggaatgg acggacccgc ttccagcact tcctgaccca gttcgttacg
    22801 catcgcacga acacgaccgt attcgaagcc acacagaacg atatcgccct tgtgcagagt
    22861 accttcacgt accagaacgg tagcaaccgg accacgacct ttatcgagga aggattcgat
    22921 aaccgcaccg ctcgccatac ctttacgtac cgctttcagc tccagaactt ccgcctgcag
    22981 caggatagcg tccagcagtt catcgatgcc ggtacccgct ttcgcagata cgtgtacgaa
    23041 ctggctttca ccgccccact cttccggcag gatgccatac tgggagagtt cgttcttaac
    23101 acgatccgga tcggcttctg gtttatcaat cttgttcact gcaaccacca ccggtacctg
    23161 cgccgctttc gcgtgctgga ttgcttcgat ggtctgcggc atcacgccgt cgtcggcagc
    23221 aacaaccagg actacgatgt ccgttgcctg cgcaccacgt gcacgcattg aagtaaacgc
    23281 ggcgtgcccc ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc
    23341 accaatgtgc tgggtaatgc cgcccgcttc gccagaggca actttcgttg aacgaatgta
    23401 gtccagcaga gaggttttac cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg
    23461 cggttcagcc gcagcacccg tgtcacggtc gctcattacc gcctcttcca gctcgttttc
    23521 acgacgcagg ataactttgt ggcccatctc ttcagcaacc agctgtgcgg tttcctgatc
    23581 gataacctgg ttgatggttg ccattgcgcc cagtttcatc atcgctttga tgacctgaga
    23641 gcctttaacc gccatcttgt tcgccagttc gccaacggtg atggtttcgc cgatcacaac
    23701 gtcacggtta acggcctgag ctggcttctg gaagccttgc tgcagggaag aacctttacg
    23761 ttttccgcct ttaccgccac gtactgctgc gcgtgcttct tcacgatcag cttttgattc
    23821 agcgtgtttg ttgcctttct tcggacgcgc tgctttcgcg ttacgaccac ggccacgacc
    23881 gccttcgact tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag aagtagtgac
    23941 gtgataatcg ctggaatctt cagtcggttc cgcgttatca gtccatttgt tttcttccgc
    24001 catacgacgt gcttcttcag caacgcgacg tgcttcttct tcgagtttac gatgcgcttc
    24061 ttcttcggct ttacgcttga gctctgcagc ttcctgctca cggcgggctt tttcagcctg
    24121 ggcgttttta gtcatatcgt cttgttgatt gctcactttg tctttttccg cagcttcacg
    24181 tttcgcttgt tcagcagctt cacgcttagc ttgttctgca gcctcacgtt cagctttttg
    24241 ttgcgcctcg cgtttagccg attcttctgc ctcacgacgg gcttgctctt ccgcttcacg
    24301 ctgcgcttgc tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt
    24361 gcgtttcttg cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt
    24421 aagggtgctg cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt
    24481 caggtggtca atcaaagtct gtttctcttg tgcagacaca gagtcgtcag cagacttccg
    24541 gatacctgca tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc
    24601 cagcgtttta atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc
    24661 gtcaccgaac cagcaaatat tacgggcagc cataatcagt gctccggcct tttcgtcggt
    24721 caacccttcg atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca
    24781 aacgccacgg gcggccagtt tgaatgccaa atcacgatct accccttcaa ggttcagcag
    24841 atcgtcagcc ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa tggtggccag
    24901 tgcattttta gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc
    24961 caacagctct ttcatcggca cataggccag ttcttccagc gtcgagaagc cttcttctac
    25021 cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc
    25081 gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg gtcatcacgt tgagttccca
    25141 accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag
    25201 gttaccggct tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc
    25261 aacgtctgcc ggtgccattg cgttaatcac gaactgcgcc gggttatcat cccacaggac
    25321 gatatcgata cgctcgccac ccagttcagt agacaccgcc tgaacacgcg cgccacgcat
    25381 acctacgcaa gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc
    25441 acgagaaccc ggatcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttctgg
    25501 cacttcaata cggaacagtt cgatcagcat ttccggcttg gaacgagtga cgaacagttg
    25561 cgcgccacgc gcttccgggc gaacggaata aagcacgcca cgaacgcggt cgccagggcg
    25621 gaagttttca cgcggcagca tatcttcacg caggatcacg gcttcagcgt tgttgcccag
    25681 atccagagag atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc
    25741 gtgttcacgg aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat
    25801 aacctgtttt gccgtctggg tagtgatacg gtcaaaggta acagactcaa tctgatcttc
    25861 aacgtaatcg cccaggttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc
    25921 cttggtcggc tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc
    25981 gcttttgcga tcgatctgta cgcggacgtc gatctcttgt tcatattttt tctttgttgc
    26041 tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt
    26101 ggatacggct tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc
    26161 agactattaa aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc
    26221 atctttacct tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg
    26281 ccatttacga cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa
    26341 acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag
    26401 gttataagca acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac
    26461 atcagcacaa tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga
    26521 tgtgcgaccg cgaataaatt cgatgccaac cagttcaaaa cccagggcct caactggcgc
    26581 agtaatcatc tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa
    26641 aaagggccta aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg
    26701 ctttatataa ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt
    26761 tttcaaatcc cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg
    26821 gaaagtggtt gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc
    26881 taccaggctg ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa
    26941 atgcaaatac tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata
    27001 gtgatccacg ccacattttg tcaacgttta ttgctaatca tgtgaatgaa tatccagttc
    27061 actttcattt gttgaatact tttgccttct cctgctcccc ct
//
LOCUS       DAZPAC010000040        26864 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZPAC010000040 DAZPAC010000000
VERSION     DAZPAC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.040"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            202..474
                     /gene="asr"
                     /locus_tag="ACHUPM_001371"
     CDS             202..474
                     /gene="asr"
                     /locus_tag="ACHUPM_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR0869197.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="ACHUPM_001372"
     CDS             567..650
                     /locus_tag="ACHUPM_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869198.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="ACHUPM_001373"
     CDS             750..1571
                     /locus_tag="ACHUPM_001373"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR0869199.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHUPM_001374"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHUPM_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR0869200.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHUPM_001375"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHUPM_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR0869201.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHUPM_001376"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHUPM_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR0869202.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHUPM_001377"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHUPM_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR0869203.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHUPM_001378"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHUPM_001378"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR0869204.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHUPM_001379"
     CDS             7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHUPM_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR0869205.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="ACHUPM_001380"
     CDS             8347..9729
                     /locus_tag="ACHUPM_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0869206.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="ACHUPM_001381"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="ACHUPM_001381"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR0869207.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="ACHUPM_001382"
     CDS             complement(10485..10820)
                     /locus_tag="ACHUPM_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR0869208.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="ACHUPM_001383"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="ACHUPM_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR0869209.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="ACHUPM_001384"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="ACHUPM_001384"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR0869210.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="ACHUPM_001385"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="ACHUPM_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR0869211.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHUPM_001386"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHUPM_001386"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR0869212.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15521..17167)
                     /gene="fumB"
                     /locus_tag="ACHUPM_001387"
     CDS             complement(15521..17167)
                     /gene="fumB"
                     /locus_tag="ACHUPM_001387"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0869213.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17366..18541
                     /gene="manA"
                     /locus_tag="ACHUPM_001388"
     CDS             17366..18541
                     /gene="manA"
                     /locus_tag="ACHUPM_001388"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR0869214.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18642..20150
                     /locus_tag="ACHUPM_001389"
     CDS             18642..20150
                     /locus_tag="ACHUPM_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR0869215.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20331..21641)
                     /gene="uidC"
                     /locus_tag="ACHUPM_001390"
     CDS             complement(20331..21641)
                     /gene="uidC"
                     /locus_tag="ACHUPM_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR0869216.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21680..23053)
                     /gene="uidB"
                     /locus_tag="ACHUPM_001391"
     CDS             complement(21680..23053)
                     /gene="uidB"
                     /locus_tag="ACHUPM_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFR0869217.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGFCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(23050..24861)
                     /gene="uidA"
                     /locus_tag="ACHUPM_001392"
     CDS             complement(23050..24861)
                     /gene="uidA"
                     /locus_tag="ACHUPM_001392"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR0869218.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25250..25840)
                     /gene="uidR"
                     /locus_tag="ACHUPM_001393"
     CDS             complement(25250..25840)
                     /gene="uidR"
                     /locus_tag="ACHUPM_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR0869219.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(26061..26828)
                     /gene="hdhA"
                     /locus_tag="ACHUPM_001394"
     CDS             complement(26061..26828)
                     /gene="hdhA"
                     /locus_tag="ACHUPM_001394"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR0869220.1"
                     /translation="MRHAPCAMRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6740 a         6956 c         6464 g         6704 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgat ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tagctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggccgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatga aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc ggctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg gcggctctgt ttatttcaca cagcgagtgc
    15541 actgtgtgag ttgtatctgc tggaagaagt catttccttt atcatccaca aggataaacg
    15601 ccgggaaatc ttccacttca attttccaga tggcttccat tcccagttcc ggatattcaa
    15661 cacattccag gctcttaata cttccctgcg ccaatacagc ggccggacca ccgatactgc
    15721 caaggtagaa gccgccgtgt tttttacagg catccgtcac ctgctggctg cggttgcctt
    15781 tcgccagcat gatcatactt ccgccctgcg cttgcagttg atcgacataa gaatccatcc
    15841 gtccggcggt cgttgggcca agagaaccgg aggcataacc ttccggcgtt ttggccggac
    15901 ccgcgtagta aatcggatga tctttaatgt actgcggcag cccttcaccg ttatccatcc
    15961 gctctttcag tttggcgtga gcaatatcac gaccgacgat aatcgtgccg ttaagcgata
    16021 agcgtgtaga aacgggatac tgcgacaact gtgcgaggat ctctttcatc ggacggttaa
    16081 ggtcaacgcg caccgcttcg ccttctcccg ctttacgcag ctcttcaggg atatatttac
    16141 ctggattatg ttccagtttt tcgatccaga tcccctgacg gttgatcttc gctttgatat
    16201 tacggtcagc agagcaggaa acacccatac caaccggaca ggatgcgccg tgacgtggca
    16261 ggcgaatcac gcgtatgtcg tgagcgaagt atttaccacc aaactgcgca cccagaccga
    16321 gattttgcgc ttcgatcagc aattcttttt ccagttccac atcgcggaat gcctgaccgt
    16381 gctcattccc ttccgttggc agttcgtcat agtatttagc ggaagccagt ttcactgttt
    16441 taaggttagt ttctgcagaa gttccaccaa ttacgaacgc aatatgatac ggaggacagg
    16501 ccgccgtacc cagcgtgcgc atcttctcaa ccaggtaatt tttcagtttc cccggcgtta
    16561 gtaacgcttt ggtttcctga tagagatacg tcttgtttgc cgaaccacca cctttggcga
    16621 tacagaggaa tttgtactcg tcgccatcaa cggcataaag atcgatctgc gctggcagat
    16681 tggtgccggt attcacctct ttatacatat ccagcggcgc gttttgcgag tagcgcaaat
    16741 tatcttcgat ataagtgtta tagacaccgc gcgccagcgc cgcttcatca cagccaccgg
    16801 tccatacacg ctgccctttt ttaccaacaa taatcgcagt gccggtatcc tgacaggttg
    16861 gcagaacacc tttcgccgcg atgtcggagt tgcgcaggaa ttgcagcgcc acatatttat
    16921 cattttcgct ggcttccggg tcacgcagaa tgtcggccac ctgttgttgg tgagccggac
    16981 gcagcataaa tgacgcatca tgaaacgcct gacgcgccaa cagagttaac gcttcgggtg
    17041 cgactttcaa aatctcctgc ccttcaaatt cagatacgct aacgtgttcg ctggttagca
    17101 ggtaatactc agtatcatct tttttgagtg gaaaaggagc ctgataatga aagggtttgt
    17161 ttgacattgt tctctcactt actgcctggt ttggttatgc tctgggcggg tgttccgttg
    17221 ccctgttaaa agcgagtaac aatatcctac acactttttt aacaaaaact gagactagta
    17281 cgactttttg cggctccagg ttacttcccg taggattctt gctttaatag tgggattaat
    17341 ttccacatta aaacagggat tgatcatgca aaaactcatt aactcagtgc aaaactatgc
    17401 ctggggcagc aaaacggcgt tgactgaact ttatggtatg gaaaatccgt ccagccagcc
    17461 gatggccgag ctgtggatgg gcgcacatcc gaaaagcagt tcacgagtgc agaatgccgc
    17521 cggagatatc gtttcactgc gtgatgtgat tgagagtgat aaatcgactc tgctcggaga
    17581 ggccgttgcc aaacgctttg gcgaactgcc tttcctgttc aaagtattat gcgcagcaca
    17641 gccgctctcc attcaggttc atccaaacaa acgcaattct gaaatcggtt ttgccaaaga
    17701 aaatgccgca ggtatcccga tggatgccgc cgagcgtaac tataaagatc ctaaccacaa
    17761 gccggagctg gtttttgcgc tgacgccttt ccttgcgatg aacgcgtttc gtgagttttc
    17821 cgagattgtc tccctactcc agccagtcgc aggtgcacat ccggcaattg ctcacttttt
    17881 acaacagcct aatgccgaac gtttaagcga actgttcgcc agcctgttga atatgcaggg
    17941 tgaagaaaaa tcccgcgcgc tggcgatttt aaaatcggcg ctcgacagtc agcagggtga
    18001 accgtggcaa acgattcgtt taatttctga attttacccg gaagacagtg gcctgttctc
    18061 cccgctgtta ctgaatgtgg tgaaattgaa tcctggcgaa gcgatgttcc tgttcgctga
    18121 aacaccgcac gcttacctgc aaggcgtggc gctggaagtg atggcgaact ccgataacgt
    18181 gctgcgtgcg ggtctgacgc ctaagtacat tgatattccg gaactggttg ccaatgtgaa
    18241 attcgaagcc aaaccggcta accagttgtt gacccagccg gtgaaacaag gtgcagaact
    18301 ggacttcccg attccagtgg atgattttgc cttctcgctg catgacctta gtgataaaga
    18361 aaccaccatt agccagcaga gtgccgccat tttgttctgc gtcgaaggcg atgcaacgtt
    18421 gtggaaaggt tctcagcagt tacagcttaa accgggtgaa tcagcgttta ttgccgccaa
    18481 cgaatcaccg gtgactgtca aaggccacgg ccgtttagcg cgtgtttaca acaagctgta
    18541 agagcttact gaaaaaatta acatctcttg ctaagcttat taaaggctta taacaccttc
    18601 aggcggccag tccgcctgat ttcattttat ggataatcat tatgaataaa tcgctggtag
    18661 cggtaggcgt cattgttgcg ctaggcgtag tctggacagg cggcgcatgg tatacaggca
    18721 agaagattga aacccatctc gaagacatgg tcgcgcaggc gaacgcgcaa ctcaaactga
    18781 cagctcctga atccaacctg gaagtgagtt atcaaaacta tcatcgcggc gtattcagca
    18841 gccagttgca actgttggtg aaacccattg ccgggaaaga aaatccgtgg attaaaagcg
    18901 gtcagagcgt catcttcaac gaatcggttg atcatggtcc cttcccgctt gcccagctta
    18961 aaaaactgaa cctgatcccg tcgatggcat caattcaaac cacgctggtt aataacgaag
    19021 taagcaaacc actgtttgat atggcaaaag gtgaaacgcc ttttgagatt aactcgcgca
    19081 ttggttacag cggtgattcc agttccgata tttcgctcaa gccactgaat tacgagcaaa
    19141 aggatgaaaa agtcgccttt agcggcggcg agttccagtt aaatgctgac agagacggca
    19201 aagccatctc cctttccggg gaggcgcaaa gtggtcggat agacgcagtt aacgaataca
    19261 accagaaagt gcagttgacc tttaataatc tgaaaaccga cggttccagc acgctggcaa
    19321 gttttggtga gcgcgtagga aaccaaaaac tgtcactgga aaaaatgacc atttcagtgg
    19381 aaggcaaaga actggcactg ctggaaggca tggagatcag cggtaaatcg gatctggtca
    19441 atgacggtaa aacgatcaat agccaactgg attactcgct aaacagcctg aaggtacaga
    19501 atcaggatct gggcagcggc aagctgactt taaaagtcgg ccagattgat ggtgaagcct
    19561 ggcatcagtt tagccagcaa tataacgcgc aaactcaggc gctgctggca cagccagaaa
    19621 ttgccaacaa tcccgaactt tatcaggaga aagtgacgga agccttcttt agcgccctgc
    19681 cgctgatgtt gaaaggcgat ccggtgatta ctatcgcgcc gctaagctgg aaaaacagtc
    19741 agggtgaaag tgcgctgaat ctgtcgctgt tcctgaaaga tccggcaacg actaaagaag
    19801 cgccgcaaac gctggcgcag gaagtagatc gttcggttaa atctctggat gcgaaactga
    19861 ccattccggt ggatatggca actgagttta tgactcaggt agcgaagctg gaaggttatc
    19921 aggaagatca agcgaaaaaa ctggcgaaac agcaagttga aggtgcatca gcaatggggc
    19981 agatgttccg tctgaccacc ttgcaggaca ataccatcac caccagcctg caatatacta
    20041 acggtcagat aacgttaaac gggcagaaaa tgccactgga agatttcgtt ggtatgtttg
    20101 cgatgccagc tcttaacgtt ccggctgtac cggcaattcc gcagcagtaa ttcacggcca
    20161 aaacgccgga gaattttcac ttcggcgttt tcatctgaaa acaaaccaga gattgcctga
    20221 tgcgctacgc ttatcaggcc taccttttcc tgcaacactt tgaatttatg agtttttgta
    20281 ggctggataa ggcgtttaca ccgcatccgg cataaaaaac gcgcactttg tcaacaatct
    20341 gaaacgccgg aggtttttct ctccggcgtt atttctgact tcagcataaa gtcatactga
    20401 atactcattt tcacatccca gtaattcagg cgatcgccca gcgtactgcc atcgcgcttt
    20461 tcccgccctt tatcggcagc aagaaagaaa ttcatccctt gtaacggccc ttcgctgacc
    20521 cgatagttca taccacctaa gatacccgcg ccttcaatcc gcacatctcg tttactggat
    20581 tcatagccgc gagtcacaat ctgtgcaaat gtcagtgtaa attgcggcgt taaacgatag
    20641 ttgacgccca attgccagga ctgcattcct tcacggttac gatcaagact taatgaaaag
    20701 ggatagccaa tgtcggtatc caccacggca ccattcattg catggcgcaa ataaccacca
    20761 ctgccaaata cgcttcccca cggcgcattc caggtcaatt gtccactaac caacgccgtt
    20821 tcattgggct ggctggtatt acgacttaaa ccttcgagct gcgcgtaaaa gccgagcaac
    20881 tcccctttca acgtataatt cgggtcgagc ggtatgcttt tctcgagaat actttgtatc
    20941 tgattggaaa catctttctg taccgatgcc cccagagaat agcgactggt atcatcatgc
    21001 caactggcgg ctaaaaaaga acgtgcgcga tgtttatcgt attttcccgt ctggctgtta
    21061 tacacccctt catcacgaat accggttgtt gaatcagtcc ataaaaatac tcgtgaacgc
    21121 cacattcccc acagatcaat atttggtgtt aaagcccaat aacctaatgc tccttcagaa
    21181 actgtcggta atgtacgttg cgaactgcca gccattaacg gaatacgata aggtaagcta
    21241 ccataactat aatccgtccc gaagcgacct gatttaagca ggctattttc actgggtttt
    21301 atttttactg cccccagggc aaagccaaaa cttttatcac catcaagata ccaccgggta
    21361 ctcatatcag cacgagcacc taatttataa acataatatg ccccgccttc aacgccaata
    21421 atattggaat aataaccact attgaaatcg agcaatcctc cctgtaccca tgcgtaaata
    21481 tctcttcccg cgccagcact gggcttatcc gccctgcgca attcgttttt cagtctgatg
    21541 cgtagtcccg cctcatcgtc agccagttgc gccgcataag cagagataag gccagaggca
    21601 gccgtcaggc aaataacggc catggccact atttttctca tagttgcacc tttgatgttc
    21661 tgattatttt tattgattat taattagtga tatcgctgat taattgctgc tgcacttttt
    21721 tacgattatc aatttcaacc acgatttctt tgaatttttt atccgtaagc ggataaaacc
    21781 agataataac gaatgccagt agcataaatc cgcaaggtac taaggcaatt gatgtgcgga
    21841 tgcccataat aacttccggc gtttgcacct gattggcaat atatccgctt aaccccaaaa
    21901 taaaggcagg aattgaacct ccgattgcct gaccgcattt acgggtaaat gagaatagtg
    21961 aataggtgag cccttcaatt cgcacgccgg tcaggtattc accgtattct acggtatcag
    22021 cttccagtgc ccacatcact gtcatagtaa cgccctgacc aattgaagcg atggccaacg
    22081 caaccaacgc caccggcagt gaccagacgg aaacccagaa gaatagcaga taaccgcagg
    22141 ttcccagcaa agcgccaatc aggaaggtat tctttttacc gatcctcgcg accatccccg
    22201 gcacaagcgg tgccgatgcc acagtaccaa ccaggttttg caccagtacc agcacagtga
    22261 acagcccggt atcatttaac acatagcgca cgtagaacaa cgacgaggcg ctgaccgcaa
    22321 aggtcgaaat cagcacacac agcgcaccga tgcacaacat aaacagcggg cgattccgtt
    22381 tcagggtttg caaactgatc ttcaatgacg gctgcgcaac gatacgtacc acattctcac
    22441 gcgtcgattt gaagcagatg aagtaaagca ccattccggc aatcgccagc acaatcgtcc
    22501 agaaatggta taccgacacc atctcttccg ggctggagtt cttaatgctc ggccctatca
    22561 gaaatgccag gcagacaaag gtcaatgaag cggcaatccc acgagccgcg cccagacggg
    22621 cgcgggattg tggttgttgg gtcatcgcgg tagcaagtga accataagga atattcacca
    22681 ggctgtagca aaacccgagg cccatgtagg tcaaatatgc atacactact ttgcggctat
    22741 ggctccagtc ggtcggtacc cagaatacca gcacgctgaa gatcattaac ggcgcagtac
    22801 cgaagagtaa aaacgggcgg aattttcccc agcgggtatt cacactgtcc accactcgtc
    22861 cggcaaagac gtcggcgaag gcatcgaata cccgcaccag taacagcatg gtgcccgccg
    22921 cagcggcacc gacgccagcg acgtcggtgt agtaactcaa caggaagagc acccccattg
    22981 cgaaggcgaa gttattggcg acgtcaccga ggctgtagcc gacgatggtg cgccaggaga
    23041 gttgttgatt cattgtttgc ctccctgctg cggtttttca ccgaagttca tgccagtcca
    23101 gcgtttttgc agcagaaaag ccgccgactt cggtttgcgg tcgcgggtga agatcccctt
    23161 cttgttaccg ccaacgcgca atatgccttg cgaggtcgca aaatcggcga aattccatac
    23221 ctgttcaccg acgacggcgc tgacgcgatc aaagacgcgg tgatacatat ccagccatgc
    23281 acactgatac tcttcactcc acatgtcggt gtacattgag tgcagcccgg ctaacgtatc
    23341 cacgccgtat tcggtgatga taatcggctg atgcagtttc tcctgccagg ccagaagttc
    23401 tttttccagt accttctctg ccgtttccaa atcgccgctt tggacatacc atccgtaata
    23461 acggttcagg cacagcacat caaagagatc gctgatggta tcggtgtgag cgtcgcagaa
    23521 cattacattg acgcaggtga tcggacgcgt cgggtcgagt ttacgcgttg cttccgccag
    23581 tggcgcgaaa tattcccgtg caccttgcgg acgggtatcc ggttcgttgg caatactcca
    23641 catcaccacg cttgggtggt ttttgtcacg cgctatcagc tctttaatcg cctgtaagtg
    23701 cgcttgctga gtttccccgt tgactgcctc ttcgctgtac agttctttcg gcttgttgcc
    23761 cgcttcgaaa ccaatgccta aagagaggtt aaagccgaca gcagcagttt catcaatcac
    23821 cacgatgcca tgttcatctg cccagtcgag catctcttca gcgtaagggt aatgcgaggt
    23881 acggtaggag ttggccccaa tccagtccat taatgcgtgg tcgtgcacca tcagcacgtt
    23941 atcgaatcct ttgccacgca agtccgcatc ttcatgacga ccaaagccag taaagtagaa
    24001 cggtttgtgg ttaatcagga actgttcgcc cttcactgcc actgaccgga tgccgacgcg
    24061 aagcgggtag atatcacact ctgtctggct tttggctgtg acgcacagtt catagagata
    24121 accttcaccc ggttgccaga ggtgcggatt caccacttgc aaagtcccgc tagtgccttg
    24181 tccagttgca accacctgtt gatccgcatc acgcagttca acgctgacat caccattggc
    24241 caccacctgc cagtcaacag acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt
    24301 gatatcgtcc acccaggtgt tcggcgtggt gtagagcatt acgctgcgat ggatcccggc
    24361 atagttaaag aaatcatgga agtaagactg ctttttcttg ccgttttcgt cggtaatcac
    24421 cattcccggc gggatagtct gccagttcag ttcgttgttc acacaaacgg tgatacgtac
    24481 acttttcccg gcaataacat acggcgtgac atcggcttca aatggcgtat agccgccctg
    24541 atgctccatc acttcctgat tattgaccca cactttgccg taatgagtga ccgcatcgaa
    24601 acgcagcacg atacgctggc ctgcccaacc tttcggtata aagacttcgc gctgatacca
    24661 gacgttgccc gcataattac gaatatctgc atcggcgaac tgatcgttaa aactgcctgg
    24721 cacagcaatt gcccggcttt cttgtaacgc gctttcccac caacgctgat caattccaca
    24781 gttttcgcga tccagactga atgcccacag gccgtcgagt tttttgattt cacgggttgg
    24841 ggtttctaca ggacgtaaca taagggactc ctcattaaga taaaaatact ggtcaacctt
    24901 taatctgatt agttaaccaa ttctgtgttg cacacagtta tctgtgagaa acccatggaa
    24961 gagaaatgga aataaaaaga actgacaccg cgatcaaaaa accaaaaact gcaataccca
    25021 cagcgtgata ttgatcgcat taatctttaa aacggcgttg acagaagcca taaaataagc
    25081 aaccgaacgt tcgttcgagc atattgggaa cgataaactc tcatgacata tggctacggt
    25141 gaatattttt gggggggaat tctccgatcc cggctagatt gtattttgta cagggaaatt
    25201 aaagtatgaa acgcgggcac agttatcacc agcccgcgaa atgagaggat caggatgcgg
    25261 ttaagatacc gccaatcatc gcgcgtaatc cctgagcaaa cgacgcttca cgcgccagat
    25321 cttccgcttc gatatcggcc agtacaccgt aggtcagatc cagcagtaaa cgtgaagtca
    25381 ttgacgtgtt gatgtctggg cttatctcgc ctttttgctg ggcatcacgc atccgctggg
    25441 caacaaattc cgtgatcgtc atatgcttat ttttaagcat ggcggcgacc tgtgggttac
    25501 gcatcccttc cgccataatt tcgaccacca gcgcccgttg cccaaaggct tcatgggtga
    25561 gagagacaat agactcgacc atatagtcaa caaaatgaat ccctttaatc ggttcccgga
    25621 aacgagccag cgccctctcc tggtcctgta agataatcgc ctgaatcaag gcttctttgg
    25681 agatgaaatg gtgatagagc gtcccgggac taatggcgca agatttacag atggctttca
    25741 tcgaggcact gtgaaatcca ttttctgaaa aaatctctct ggcagcattg aggatccggg
    25801 tccgtgtcgg ttgtgcttca gtctgcatgt tatccatcat taaaccatta tagttagcgt
    25861 gaataactag tttaccgcgt tgtcaggtga gcgcctatca ttgctgttga atagtgcgaa
    25921 ggcacactct attcgtatac ttttcactct gaaattggtt aaccaatagc cgtgatttgg
    25981 ctgccagtta tttagcagag acgttgatga gtttatcccg gtaaacaaca cgcacggttg
    26041 tttaccggtc cgttagtgta ttaattgagc tcctgtaccc caccaccgga gacggtgaga
    26101 atttgtccgc ttacccagct cgcagcaggc gagcaaagga acagcgctgc gttagcaata
    26161 tcttgcggtt ggcccagacg tctgatcggc gtgtgctgta acattttttg ttcaatttct
    26221 ggtgtaataa cggatttcag ggcatcggtt aatattgccc ccggcgcaat gccatttacc
    26281 cgaatatttt tttcacccag gtcaaacgcc atatttctga ccagatgact ggctgcagct
    26341 ttagatgatg cataggaagt catgtttata tttttatttt ctgccgccat agaagtgatg
    26401 gtcagaataa cgccaccgcc atttttttcc atttctggcg caacaagttg tgacagatgg
    26461 aaaaaagaaa acacattcag ttcataagca cggcgaaaat ccgccattgg catatcaaac
    26521 ggtttaggtc caccgccacc ggcgttgtta accagaatat caactttacc cagcttactg
    26581 atagcaaagt ctgccagtgc agagagttcc tgttcggaag taatatcaca acggcaggca
    26641 aatgcctgac cacccagttg ttgaatttcg tctacaacat ggttagctgc gtcggcgtta
    26701 atatcactga ccaccacaga tgcgccagct gtcgcgaatg taatggcgat ttctttacca
    26761 atacctgcac ccgcacctgt gatgatggcg catggcgcat ggcgcatggc gcatggcgca
    26821 tggcgcatgg cgcatggcgc atggcgcatg gcgcattttc cgtc
//
LOCUS       DAZPAC010000041        26527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZPAC010000041 DAZPAC010000000
VERSION     DAZPAC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.041"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            9..332
                     /locus_tag="ACHUPM_001395"
     CDS             9..332
                     /locus_tag="ACHUPM_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR0869221.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(435..1685)
                     /gene="icd"
                     /locus_tag="ACHUPM_001396"
     CDS             complement(435..1685)
                     /gene="icd"
                     /locus_tag="ACHUPM_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR0869222.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1857..2510
                     /gene="rluE"
                     /locus_tag="ACHUPM_001397"
     CDS             1857..2510
                     /gene="rluE"
                     /locus_tag="ACHUPM_001397"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR0869223.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2520..2981
                     /gene="nudJ"
                     /locus_tag="ACHUPM_001398"
     CDS             2520..2981
                     /gene="nudJ"
                     /locus_tag="ACHUPM_001398"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR0869224.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3035..4141
                     /gene="mnmA"
                     /locus_tag="ACHUPM_001399"
                     /gene_synonym="trmU"
     CDS             3035..4141
                     /gene="mnmA"
                     /locus_tag="ACHUPM_001399"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR0869225.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4177..4818
                     /gene="hflD"
                     /locus_tag="ACHUPM_001400"
     CDS             4177..4818
                     /gene="hflD"
                     /locus_tag="ACHUPM_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR0869226.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4822..6192
                     /gene="purB"
                     /locus_tag="ACHUPM_001401"
     CDS             4822..6192
                     /gene="purB"
                     /locus_tag="ACHUPM_001401"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR0869227.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6360..7031
                     /gene="phoP"
                     /locus_tag="ACHUPM_001402"
     CDS             6360..7031
                     /gene="phoP"
                     /locus_tag="ACHUPM_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR0869228.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7031..8491
                     /gene="phoQ"
                     /locus_tag="ACHUPM_001403"
     CDS             7031..8491
                     /gene="phoQ"
                     /locus_tag="ACHUPM_001403"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR0869229.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8567..9688
                     /gene="roxA"
                     /locus_tag="ACHUPM_001404"
                     /gene_synonym="ycfD"
     CDS             8567..9688
                     /gene="roxA"
                     /locus_tag="ACHUPM_001404"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR0869230.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9737..10963)
                     /gene="pepT"
                     /locus_tag="ACHUPM_001405"
     CDS             complement(9737..10963)
                     /gene="pepT"
                     /locus_tag="ACHUPM_001405"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR0869231.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            11213..12349
                     /gene="potA"
                     /locus_tag="ACHUPM_001406"
     CDS             11213..12349
                     /gene="potA"
                     /locus_tag="ACHUPM_001406"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR0869232.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12333..13190
                     /gene="potB"
                     /locus_tag="ACHUPM_001407"
     CDS             12333..13190
                     /gene="potB"
                     /locus_tag="ACHUPM_001407"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR0869233.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13187..13981
                     /gene="potC"
                     /locus_tag="ACHUPM_001408"
     CDS             13187..13981
                     /gene="potC"
                     /locus_tag="ACHUPM_001408"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR0869234.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13978..15024
                     /gene="potD"
                     /locus_tag="ACHUPM_001409"
     CDS             13978..15024
                     /gene="potD"
                     /locus_tag="ACHUPM_001409"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR0869235.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(15180..16001)
                     /gene="cobB"
                     /locus_tag="ACHUPM_001410"
     CDS             complement(15180..16001)
                     /gene="cobB"
                     /locus_tag="ACHUPM_001410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR0869236.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16017..16928)
                     /gene="nagK"
                     /locus_tag="ACHUPM_001411"
     CDS             complement(16017..16928)
                     /gene="nagK"
                     /locus_tag="ACHUPM_001411"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR0869237.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(16957..18201)
                     /gene="lolE"
                     /locus_tag="ACHUPM_001412"
     CDS             complement(16957..18201)
                     /gene="lolE"
                     /locus_tag="ACHUPM_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR0869238.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(18201..18902)
                     /gene="lolD"
                     /locus_tag="ACHUPM_001413"
     CDS             complement(18201..18902)
                     /gene="lolD"
                     /locus_tag="ACHUPM_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR0869239.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18895..20094)
                     /gene="lolC"
                     /locus_tag="ACHUPM_001414"
     CDS             complement(18895..20094)
                     /gene="lolC"
                     /locus_tag="ACHUPM_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR0869240.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20356..21429
                     /locus_tag="ACHUPM_001415"
     CDS             20356..21429
                     /locus_tag="ACHUPM_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR0869241.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21557..25003
                     /gene="mfd"
                     /locus_tag="ACHUPM_001416"
     CDS             21557..25003
                     /gene="mfd"
                     /locus_tag="ACHUPM_001416"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR0869242.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            25147..26103
                     /gene="ldtC"
                     /locus_tag="ACHUPM_001417"
                     /pseudo
     CDS             25147..26103
                     /gene="ldtC"
                     /locus_tag="ACHUPM_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(26186..26443)
                     /gene="bhsA"
                     /locus_tag="ACHUPM_001418"
                     /gene_synonym="ycfR"
     CDS             complement(26186..26443)
                     /gene="bhsA"
                     /locus_tag="ACHUPM_001418"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFR0869243.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6480 a         6879 c         6716 g         6452 t
ORIGIN      
        1 cataacaaat gaagtgggta gtaattgaca cggtaattca acctacatgc ggcatatctt
       61 tttcagccat atggggtgat atgaaaaaga tcatctggta tcaatctact atatttcttc
      121 ctcctggaag tatatttaca ccggttaagc ttggtattat ccttaaggaa aaagaatatc
      181 ctattactat ttataacatt gcaccattca acaagaattt atggagttta ctcaaaagca
      241 gtcaagagtg tcctccagga gaaagcaaaa taacaaataa atgtttacat aatagttgca
      301 ttataaaaat atgcccatat gggctcaagt aatgggcttc atataaatat gctcgatatg
      361 ctattaataa tatccacata aataaaacaa cgggcgtgtt atacgcccgt ttcaatattt
      421 aactcatgga gagattacat gttttcgatg atcgcttcac caaactctga acatttcagc
      481 agcttagcgc cttccatcag acgttcaaag tcataagtta cggttttcgc gttgatagca
      541 ccttccatac ctttaacaat taaatcagcc gcttcagtcc accccatatg gcgcagcatc
      601 atctcagcgg agaggatgat agagcctgga tttactttgt cctgaccggc atatttcggc
      661 gcagtaccgt gggtggcttc aaacagggcg cattcgtcac cgatgtttgc gccaggggcg
      721 ataccgatac cgccaacctg cgctgccagg gcgtcagaaa tgtagtcacc gttcaggttc
      781 atacaggcga taacatcata ttcagccgga cgcagcagga tctgttgcag gaatgcatca
      841 gcaatcacgt ctttaatgac gatctctttg ccggtgttcg ggtttttaac tttcagccac
      901 gggccgccgt cgatcagttc accgccaaac tcttcacgcg ccagctggta gccccagtct
      961 ttaaacgcgc cttcggtgaa cttcatgatg ttgcctttgt gcaccagagt cacagagtca
     1021 cgatcgttag caattgcgta ttcgatcgct gcacgaacca gacgtttggt gccttcttcc
     1081 gaacacggct taataccgat accacaatgt tccgggaagc gaattttctt cacccccatc
     1141 tcttcacgca ggaatttaat cactttctcg gcgtcggcag agtctgcttt ccattcgata
     1201 cccgcataaa tgtcttccga gttttcacgg aagataacca tatcggtcag ttcagggtgt
     1261 ttaaccgggc ttggagtgcc ctgatagtaa cgtaccggac gcaggcagat gtagagatcc
     1321 agttcctggc gcagggcaac gttcagagag cgaataccgc caccaacagg agtggtcagc
     1381 ggacctttaa tggcaacgcg atattcacga atcagatcaa gggtttcagc aggcagccag
     1441 acatcctgac cataaacctg tgtggatttt tcaccggtgt aaatttccat ccaggagatt
     1501 ttacgctcgc ctttataggc tttctcgact gcagcgtcga ccactttcag catggctggg
     1561 gttacatcta caccgattcc atcaccttca atgtaaggga taatcggatt ttcaggaacg
     1621 ttgagtttgc cgttttgcag ggtgatcttc ttgccttgtg ccggaacaac tactttactt
     1681 tccattcacc tctccttcga gcgctactgg tttgctcgtc tgccaccacg ttgcatatgc
     1741 gtttgcgtcc tgcgatacgg atgctttaga gcaatttttt gttaatgatt tgtaattggc
     1801 ttgtcaatgc taccctattg tttgccgtca tgaaagatgc gcgttattag gctataatgc
     1861 ggcaattcat aatctctgaa aataccatgc aaaaaacttc ttttagaaat caccaggtta
     1921 agcgattcag ctcgcaacgt tctactaggc gtaaacctga aaaccagccc acgcgtgtga
     1981 tcctgttcaa taaaccctac gatgttcttc cgcagttcac cgatgaagcc ggacgcaaaa
     2041 cattaaaaga attcatcccg gttcagggcg tttatgcagc aggtcgtctt gaccgcgata
     2101 ccgaagggtt actggtgctg accaataacg gcgcgttgca ggcgcgttta acccagccgg
     2161 gcaaacgcac cgggaaaatc tattatgtgc aggtggaagg tattcccaca caagacgcac
     2221 ttgaagcctt gcgcaatggc gtaaccttaa atgatggccc taccctgccc gccggcgcgg
     2281 agctggttga cgaaccagcg tggttatggc cgcggaatcc accaattcgt gaacgcaaaa
     2341 gtattcccac cagctggctg aagatcacct tatatgaagg acgtaatcgc caggtgcgcc
     2401 gcatgaccgc ccatgttggt ttccccacgc tgcgactgat tcgttatgcg atgggtgatt
     2461 acactttgga taaccttgcc aatggcgaat ggcgagaagc aacagaataa ggatgcacaa
     2521 tgtttaaacc gcacgttacc gttgcttgcg tagtgcacgc agagggtaaa tttttagtcg
     2581 ttgaagagac gattaatggt aaagcgttat ggaaccaacc tgccgggcat ctggaagccg
     2641 atgaaacctt agtggaagct gccgcccgtg agctatggga agaaaccggc atcagcgcgc
     2701 aaccgcaaca ctttattcgt atgcatcagt ggattgcgcc agataaaacg ccgtttttgc
     2761 gtttcctctt tgccattgag cttgagcaaa tatgcccgac ccagcctcat gacagcgata
     2821 tcgactgctg tcgttgggtc agcgccgaag aaattttaaa ggcgtcaaat cttcgttcgc
     2881 cgctggtggc ggaaagtatc cgttgttatc aaagtgggca acgctatccg ctggagatga
     2941 ttggcgattt taactggcct tttacaaagg gtgtcatcta aaaggctgcg tgatagaata
     3001 cgccgccttg aagttcaatg tcgtgagtga tccaatgtct gaaaccgcaa aaaaagtaat
     3061 cgtcggcatg tccggcggtg tcgattcctc cgtttctgcc tggctgttgc aacaacaggg
     3121 atatcaggtt gaaggcctgt ttatgaagaa ctgggaagaa gacgacggtg aggaatattg
     3181 cacagcggca gcggatctgg ctgatgccca ggctgtctgc gacaagctcg gcattgaact
     3241 gcacaccgtt aactttgctg ccgagtactg ggacaatgtc ttcgaactgt tccttgccga
     3301 atataaagcc ggtcgcacgc cgaatccgga tattctgtgc aacaaagaga tcaaatttaa
     3361 agctttcctc gaatttgccg ccgaagattt aggtgccgat tatatcgcta ccggtcatta
     3421 cgtacgtcgg gccgatgtcg atggcaagag ccgcctgctg cgtggtctgg acagcaataa
     3481 agaccagagc tacttccttt atacgctcag ccatgagcag attgcgcaaa gcctgttccc
     3541 ggtcggcgaa ctggaaaaac cgcaggtgcg taagattgct gaagatcttg gtctggtcac
     3601 cgcgaagaaa aaagactcta ccggcatttg cttcattggc gaacgtaaat tccgcgagtt
     3661 cctgggccgt tatctcccgg ggcaaccggg caaaatcatt accgtcgatg gcgatgaaat
     3721 tggtgagcac caggggctga tgtatcacac tctcggtcag cgtaaaggtc tgggtatcgg
     3781 tggcaccaaa gaaggcaccg aagaaccttg gtatgtggtg gacaaagacg tcgaaaacaa
     3841 cattctggtt gtcgctcagg gccatgaaca cccgcggctg atgtctgtcg ggttgattgc
     3901 ccagcagttg cactgggtcg atcgcgaacc attcaccggc actatgcgtt gcacggtaaa
     3961 aacccgctat cgccagaccg atatcccttg caccgtcaag gcgctggacg atgatcgcat
     4021 tgaagtgatt ttcgatgaac cggttgccgc cgtgacgccg ggccagtctg ccgtcttcta
     4081 taacggtgaa gtgtgcctcg gtggcggtat tattgagcag cgtctgccgc tgccggtctg
     4141 attattatct ttacttaaaa caaggaagca gtgaacgtgg caaagaatta ctatgacatc
     4201 accctcgccc tggccggtat ttgtcagtcg gcacgcctgg tgcaacaact cgctcaccag
     4261 gggcattgtg atgccgatgc gctacacgtc tcactcaaca gtattattga tatgaacccc
     4321 agctcgacgc tggcggtttt tggcggtagc gaagccaacc tgcgcgtcgg gctggaaacc
     4381 ctgctcggcg tgctcaatgc cagcagtcgc cagggcttaa acgccgaatt aacccgctac
     4441 acactcagct tgatggtgct tgagcgcaaa ctctcctcag cgaaaggcgc gctcgacact
     4501 ctgggcaacc ggatcaacgg cctgcaacgc cagctcgaac acttcgattt acagtccgaa
     4561 acgctgatga gcgcgatggc tgctatctat gttgatgtga tcagcccgct tggcccacgc
     4621 attcaggtca ccggttcccc tgctgtactg caaagcccac aagtgcaggc gaaagttcgc
     4681 gcaaccctgc tggcaggcat tcgcgccgcc gtgctctggc accaggtcgg cggcggacgt
     4741 ctgcaactga tgttttctcg taatcgcctg accactcagg caaaacaaat tcttgctcat
     4801 ttaaccccgg agttgtgatc tatggaatta tcctcactga ccgccgtttc ccctgtcgat
     4861 ggacgctacg gcgataaagt cagcgcgctg cgcgggattt tcagcgaata tggtttgctg
     4921 aaattccgtg tacaagttga agtacgttgg ctgcaaaaac tggccgcgca cgcagcgatc
     4981 aaggaagttc ctgcttttgc tgccgacgca atcggttacc ttgatgcaat tgtcgccaat
     5041 ttcagcgaag aagatgccgc acgcatcaaa accatcgagc gtaccactaa ccacgacgtt
     5101 aaagcggttg agtatttcct gaaagaaaaa gtggcggaga tcccggaact gcacgcggtt
     5161 tctgaattca tccactttgc ctgtacttcg gaagatatca ataacctctc ccacgcatta
     5221 atgctgaaaa ccgcgcgtga tgaagtgatc ctgccgtact ggcgtcaact gattgatggc
     5281 attaaagatc tcgccgttca gtaccgcgat atcccgctgc tgtctcgtac ccacggtcag
     5341 ccagccacgc cgtcaaccat cggtaaagag atggcaaacg tcgcctaccg tatggagcgc
     5401 cagtaccgcc agcttaacca ggtggagatc ctcggcaaaa tcaacggcgc ggtcggtaac
     5461 tataacgccc acatcgccgc ttacccggaa gttgactggc atcagttcag cgaagagttc
     5521 gtcacctcgc tgggtattca atggaacccg tacactaccc agattgaacc gcacgactac
     5581 atcgccgagc tgtttgattg cgttgcacgc ttcaacacca ttctgatcga ctttgatcgt
     5641 gacgtctggg gttatatcgc ccttaaccac ttcaaacaga aaaccattgc tggtgagatt
     5701 ggttcttcca ccatgccgca taaagttaac ccgatcgact tcgagaactc cgaagggaat
     5761 ctgggccttt ccaacgcggt attacagcat ctggcaagca aactgccggt ttcccgctgg
     5821 cagcgtgacc tgaccgactc caccgtgctg cgtaacctcg gcgtgggtat cggttatgcg
     5881 ctgattgcgt atcaatccac cctgaaaggc gtgagcaaac tggaagtgaa ccgtgaccat
     5941 ctgctggatg agctggatca caactgggaa gtgctggctg agccaatcca gacagttatg
     6001 cgtcgctatg gcatcgaaaa accgtacgag aagctgaaag agctgactcg tggtaagcgc
     6061 gttgacgccg aaggcatgaa gcagtttatc gacggtctgg cgttgccaga agaagagaaa
     6121 gcccgcctga aagcgatgac gccggcaaac tacattggtc gcgccatcac catggttgat
     6181 gagctgaaat aaactcgtat cagtgccgga tggcgatgct gtccggcctg cttattaaga
     6241 ttatccgctt tttatttttt cactttacct cccctccccg ctggtttatt taatgtttac
     6301 ccccataacc acataatcgc gttacactat tttaataatt aagacaggga gaaataaaaa
     6361 tgcgcgtact ggttgttgaa gacaatgcgt tgttacgtca ccaccttaaa gttcagattc
     6421 aggatgctgg tcatcaggtc gatgatgcag aagatgccaa agaagccgat tattatctca
     6481 atgaacattt accggatatt gcgattgtcg atctcggatt gccagacgag gacggtctgt
     6541 cactgattcg ccgctggcgt agcaatgatg tttcactgcc gattctggta ttaaccgccc
     6601 gtgaaagctg gcaggacaaa gtcgaagtat taagtgctgg tgctgatgat tacgtgacta
     6661 aaccatttca tattgaagag gtgatggcgc gaatgcaggc attaatgcgg cgtaatagcg
     6721 gtctggcttc acaggtcatt tcgctaccac cgtttcaggt tgatctctct cgccgtgaat
     6781 tatctattaa tgacgaagtg atcaaactga ccgcgttcga atacaccatt atggaaacgt
     6841 tgatacgcaa taatggcaaa gtggtgagca aagattcgtt aatgctccaa ctctatccgg
     6901 atgcggagct gcgggaaagc cataccattg atgtactgat gggacgtctg cgcaaaaaaa
     6961 ttcaggcaca atatccgcaa gaagtgatta ctaccgttcg cggccagggc tatttgttcg
     7021 aattgcgctg atgaaaaaat tactgcgtct ttttttcccg ctctcgctgc gggtacgttt
     7081 tctgttggca acggctgcgg tagtactggt gctttcgctt gcctacggaa tggtcgcgct
     7141 gatcggttat agcgtcagtt tcgataaaac tacgtttcgg ctgttacgtg gcgagagcaa
     7201 tctgttctat acccttgcga agtgggaaaa caataagttg catgtcgagt tacccgaaaa
     7261 tatcgacaag caaagcccca ccatgacgct aatttatgat gagaacgggc agcttttatg
     7321 ggcgcaacgt gacgtgccct ggctgatgaa gatgatccag cctgactggc tgaaatcgaa
     7381 tggttttcat gaaattgaag cggatgttaa cgataccagc ctcttgctga gtggagatca
     7441 ttcgatacag caacagttgc aggaagtgcg ggaagatgat gacgacgcgg agatgaccca
     7501 ctcggtggca gtaaacgtct acccggcaac atcgcggatg ccaaaattaa ccattgtggt
     7561 ggtggatacc attccggtgg agctaaaaag ttcctatatg gtctggagct ggtttatcta
     7621 tgtgctctca gccaatctgc tgttagtgat cccgctgctg tgggtcgccg cctggtggag
     7681 tttacgcccc atcgaagccc tggcaaaaga agtccgcgaa ctggaagaac ataaccgcga
     7741 attgctcaat ccagccacaa cgcgagaact gaccagtctg gtacgaaacc tgaaccgatt
     7801 gttaaaaagt gaacgcgaac gttacgacaa ataccgtacg acgctcaccg acctgaccca
     7861 tagtctgaaa acgccactgg cggtgctgca aagtacgctg cgttctctgc gtagtgaaaa
     7921 gatgagcgtc agtgatgctg agccggtaat gctggagcaa atcagccgca tttcacagca
     7981 aattggctac tacctgcatc gtgccagtat gcgcggcggg acattgctca gccgcgagct
     8041 gcatccggtc gccccactgc tggacaatct cacctcagcg ctgaacaaag tgtatcaacg
     8101 caaaggggtc aatatctctc tcgatatttc gccagagatc agctttgtcg gtgagcagaa
     8161 cgattttgtc gaggtgatgg gcaacgtgct ggataatgcc tgtaaatatt gcctcgagtt
     8221 tgtcgaaatt tctgcaaggc aaaccgacga gcatctctat attgtggtcg aggatgatgg
     8281 ccccggtatt ccattaagca agcgagaggt cattttcgac cgtggtcaac gggttgatac
     8341 tttacgccct gggcaaggtg tagggctggc ggtagcccgc gaaatcaccg agcaatatga
     8401 gggtaaaatc gtcgccggag agagcatgct gggcggtgcg cggatggagg tgatttttgg
     8461 tcgccagcat tctgcgccga aagatgaata aatatgtcca tacttcacgc attacgttaa
     8521 gcatccgtta taatcggttg cagataccag cctgtggatg cttaacatgg aataccaact
     8581 cactcttaac tggcccgatt ttcttgaacg tcactggcag aaacgcccgg tggtgttaaa
     8641 acgcggcttt aataatttta ttgacccgat ctctccagac gagttggcgg gtctggcgat
     8701 ggaaagcgaa gttgacagtc gactggtcag tcaccaggat ggcaaatggc aggtcagcca
     8761 cggcccgttc gaaagctacg atcatctcgg tgaaaccaac tggtcattac tggtacaggc
     8821 agtgaaccac tggcatgagc cgaccgccgc gctgatgcga ccgttccgtg aactaccgga
     8881 ctggcgtatt gatgatctga tgatttcttt ttctgtacct ggcggcggcg tcggcccgca
     8941 tctcgatcag tacgacgtgt ttatcattca gggtaccgga cgtcgtcgct ggcgagtggg
     9001 cgaaaagctg caaatgaaac agcactgccc acatccggat ctgttacagg tcgatccgtt
     9061 cgaagccatc atcgatgaag agctggagcc tggtgatatt ctttatattc cgccaggatt
     9121 cccgcatgaa ggctacgcgc tggaaaatgc gatgaactat tccgtgggct ttcgcgcgcc
     9181 aaatacgcgg gaactgatta gtggatttgc cgattatgtg ctgcaacgtg aactgggcgg
     9241 caactactac agcgatcctg atgttccacc tcgcgctcat cctgcggatg ttctgcctca
     9301 agagatggat aaactgcgtg agatgatgct cgaattgatc aaccagccgg aacactttaa
     9361 gcaatggttt ggcgagttta tatcccagtc acgtcatgaa ctggatatcg cgccgccaga
     9421 accgccttat cagccagatg aaatctacga tgcgctgaaa caaggtgatg tgctggtgcg
     9481 cctgggtggt ctgcgcgtat tgcgcattgg cgacgacgtg tatgccaatg gtgagaagat
     9541 cgattccccg caccgtccgg cactggatgc actcgccagc aacattgcgc tgactgcgga
     9601 gaattttggc gatgcgctgg aagatccgtc attcctcgcg atgctcgcgg cgctggtcaa
     9661 tagcgggtat tggttcttcg aagggtaagt ttggatttaa gccggatgcg gcatcacatg
     9721 ccgcatccct tttcgcttac ttccgttgcg ccgttaactc ggcaatacgg acgatcacct
     9781 gcaccgcttt ttccatacct tccagagtca caaactcatg cttaccatga tagttgtaac
     9841 cgccagtgaa caagttcggg cacggtaatc ccataaacga caactgcgcg ccgtcggtac
     9901 caccgcggat cggtttcagt tccggttcaa tatcgcagtc acgcatcgcc tgctgggcga
     9961 tatcgagaat atgcggatgc tcaaccactt tctctcgcat attgtagtaa ctgtcttcaa
    10021 tcaccagttc aatgtagcaa tcaggatgta accctttgcc cacttttttg gcgatctcca
    10081 tcattttacg tttacgcgct tcaaactgtt tacggtcgaa atcacggatg atgtagtgca
    10141 tatcggcccg ttcaacggtg cctttcatgc tcgccaggtg atagaaacct tcatagcctt
    10201 ctgtcatttc cgggctttca tccgccggaa cttccgcatg aatacgtgcc gccagcgaca
    10261 gcgcatttac catcactcct ttcgccgtgc ccggatgaac attgttaccg acaattttga
    10321 tattgaccga tgcggcgttg aagttttcaa actccagttc gcctacgcca ccaccgtcaa
    10381 cagtgtaagc ccagcgggca tcgaaggcat caacatcaaa atgtttcgcc cctttgccca
    10441 cttcttcatc cggggtaaag gcgacgcgaa tatcaccatg cggaatgttt ttctgttgca
    10501 ataccgccag cgcggtcatg atttctgcaa tacctgcttt gtcatcggca cctaacaagg
    10561 ttttaccatc ggtggtaatc aacgtctgac ccagtagctg atgcagcacc gggaacataa
    10621 ccggtgataa aacttcatcg ccgataccca gcgcaatatc gccaccgcga tagttttcaa
    10681 caatttgcgg attaacattt ttgccgctgc aatccggtga ggtatccaca tgagaaataa
    10741 agccaatcgc cgggatatcg ccagggacgt tagccggtaa cgtcgccatc aaagtgccct
    10801 tctcacttaa ggtcacattg ataagcccca tttcttcgag ctgctctttc agcagatgca
    10861 ataacttcca ttggccttcc gtgctgggaa cctgtctcac ccctgctttt gattgggtat
    10921 ccagagatac gtagttcaaa aatcgctcaa gtagtttatc catgtagtca ccctcacttt
    10981 ttgtgacaac attattaaga agcaagaaaa gacaaatatt gcgtcaggtc acttttaccc
    11041 ctgcaagcgg gaatatttat cagcattacc ttaatgaata taaagctaag ccagtaattc
    11101 acaacaaaga ttggcgattc aagcggtttg ccgtagaatt accgccctca ttaagagtca
    11161 ccaaggtggt taaccacaaa ccccgcatcg gtaagccatc cgttgcgttt acatgggaca
    11221 gagtaaaaaa ttgaataaac aaccgagttc gctttcaccg ctggtgcaat tggcgggaat
    11281 tcgcaaatgc tttgatggta aagaggtcat tccccagctg gatctgacta tcaacaatgg
    11341 cgagttcctc acgctgcttg gcccttctgg ctgcggtaaa acaaccgttc ttcgcctgat
    11401 tgcaggtctg gaaactgttg attccggacg catcatgctg gataacgagg acatcaccca
    11461 cgttccggcg gaaaaccgct atgtgaacac tgttttccaa agctacgcac ttttccccca
    11521 catgaccgtg ttcgaaaatg tggcctttgg gttgcgcatg caaaaaaccc ccgctgctga
    11581 aattacgccc cgcgtgatgg aagccctgcg gatggtgcaa ctggaaacct tcgctcaacg
    11641 taaaccgcat cagctctctg gtggtcaaca gcaacgcgtc gccattgctc gcgcggtggt
    11701 taacaagcct cgtctgttgt tgctggatga gtcgctctca gcgctggatt acaaactgcg
    11761 taagcaaatg cagaacgagc tgaaagcgtt acagcgtaag cttggtatta ccttcgtctt
    11821 tgtcactcac gatcaggaag aagcgctcac catgtcagac aggattgtgg tgatgcgcga
    11881 gggtcgtatt gagcaagatg gtacaccgcg cgaaatttac gaagagccga aaaacctgtt
    11941 tgttgccggc ttcattggcg aaatcaatat gtttaacgcc actgtcatcg aacgtctcga
    12001 cgagcagcgc gtacgcgcca acgttgaagg ccgcgaatgt aatatctacg ttaacttcgc
    12061 cgttgaaccg gggcaaaaac tgcatgttct gctgcgcccg gaagacttac gtgttgaaga
    12121 gattaacgac gacaaccacg ctgaagggct gattggttac gttcgtgagc gtaactacaa
    12181 aggcatgacg ctggagtcgg ttgttgaact ggaaaatggc aagatggtga tggtcagcga
    12241 attcttcaat gaagacgacc ctgactttga ccactctctc gaccaaaaaa tggccattaa
    12301 ttgggtagaa agctgggagg tcgtactggc tgatgaagaa cacaagtaag ttccagaatg
    12361 tagtgattgt cactattgtc ggttggcttg tgttgtttgt ctttctgccc aacctgatga
    12421 tcattggcac cagctttttg acccgcgacg acgccagttt cgtcaaaatg gtctttacgc
    12481 tggataacta cacgcgtctg ctcgatccgc tctattttga agtgctattg cactcgctga
    12541 atatggcgct gatcgccacc ctcgcctgcc tggtgctggg ctacccgttt gcctggtttc
    12601 tggcgaagct gccacacaag gtgcgtccgc tgctgctgtt tctgctgatt gttcccttct
    12661 ggaccaactc actgattcgc atctacgggc tgaaaatttt cctcagcacc aaaggctatc
    12721 tcaacgagtt tttgctctgg ctgggcgtta tcgacacacc aattcgtatc atgttcacgc
    12781 ccagtgcggt gattatcggt ctggtttaca ttctgctacc gtttatggtg atgccgctgt
    12841 actccagtat cgaaaaactg gataagccgt tgcttgaggc ggcgcgcgat ctcggtgcca
    12901 gcaagttaca gacttttatc cgtatcatta ttccactgac gatgccggga attattgccg
    12961 gatgtctgct ggtgatgctg ccagcgatgg gcctgttcta tgtatccgac ctgatgggcg
    13021 gtgcgaaaaa cctgctgatc ggtaacgtca tcaaggtcca gttcctcaat attcgtgact
    13081 ggccgtttgg tgcagccacc agcattacgc tgactatcgt aatgggcctg atgttgctgg
    13141 tttactggcg cgcttctcgt ctgctgaata aaaaggtgga actcgaatga tcggtcgact
    13201 gcttcgcggc ggttttatga ccgctatcta cgcgtacctg tatatcccaa tcattatttt
    13261 gattgtgaac tcctttaaca gctcgcgctt tggcatcaac tggcagggtt ttaccaccaa
    13321 atggtatagc ctgctgatga acaacgacag cctgttacag gcagcgcagc attcactaac
    13381 aatggcggtg ttttcggcga catttgctac gcttatcggt tcactgacgg cagttgcgct
    13441 gtaccgttat cgctttcgtg gtaagccgtt cgttagcgga atgctgtttg tggtgatgat
    13501 gtcaccagat atcgtgatgg cgatttctct gctggtgctg tttatgctgc tgggtattca
    13561 gcttggcttc tggtcgctgc tgttctcgca tatcaccttc tgcctgccgt ttgtggtggt
    13621 gacggtgtat tcgcgcctga aaggttttga cgtgcggatg ctggaagcgg cgaaagatct
    13681 cggtgccagt gaatttacca ttctgcgaaa aatcattctg ccactggcaa tgccagcggt
    13741 ggcggcgggc tgggtgttaa gctttaccct gtcgatggac gatgtggtag tttcttcgtt
    13801 cgtcaccgga ccaagttatg aaattctgcc gttaaaaatt tattcgatgg tcaaagtcgg
    13861 cgtatcgccg gaagttaacg cgctggcaac catattactg gtgctgtcgc tggtgatggt
    13921 tattgccagc cagcttattg ctcgtgataa aacgaaaggt aacacagggg acgttaaatg
    13981 aaaaaatggt cacgccacct gctcgcggcg ggtgctctgg cactgggcat gagcgccgct
    14041 cacgccgatg acaacaacac gctgtatttc tacaactgga ccgagtacgt gccgccagga
    14101 ctgcttgaac agttcaccaa agaaaccggt attaaggcta tctattcgac ttacgagtcg
    14161 aacgaaacca tgtacgccaa gctgaaaacg tacaaagacg gtgcctatga cctggtggtt
    14221 ccttcaacct attacgtcga taaaatgcgt aaagaaggga tgatccagaa gatcgacaag
    14281 tcgaagttaa cgaacttcag caatctcgat ccagacatgc tcaacaagcc gtttgacccg
    14341 aataacgact actccattcc gtatatctgg ggtgcgacgg cgattggcgt taacggtgat
    14401 gcggtggatc ctaaatctgt caccagttgg gcagatctgt ggaagccaga gtacaaaggt
    14461 agcctgctgc tgaccgacga tgcccgtgaa gtgttccaga tggcgctgcg caaactgggc
    14521 tactccggta acaccaccga tccgaaagag attgaagctg catataacga gctgaaaaaa
    14581 ctgatgccaa acgtcgcagc gtttaactcc gataacccgg ctaacccgta catggaaggc
    14641 gaagttaacc tcggcatgat ctggaacggc tctgctttcg ttgcacgcca ggcgggtacg
    14701 ccaattgacg tggtgtggcc gaaagaaggc ggcattttct ggatggacag cctggcgatc
    14761 ccggcaaatg ccaaaaacaa agaaggcgcg ctgaaattga tcaacttcct gctgcgcccg
    14821 gatgtggcaa aacaggttgc cgaaactatc ggttatccaa cgccaaacct tgcggcgcgt
    14881 aagctgttaa gcccggaagt ggcgaacgat aaaacgctct acccggatgc tgaaaccatt
    14941 aaaaatggtg aatggcagaa tgacgttggc gcagccagca gcatttatga agagtattat
    15001 cagaagctga aagcaggacg ttaattcagc aaacgggcgg tggtattacc gcccgttttt
    15061 tagttagcaa ggatgtggct aatgagtcag gacagtaaag ttttatcccg ctctttcgtc
    15121 ggtattgtag tccccttgta ggcctgataa gcgtagcgca tcaggcaatg cttcctgctt
    15181 tataatccct tcagcaactt ttcaacaaac tcaggcacca cctggcttgc cgggccgtaa
    15241 tatttctcgg caaattcatt accgacctga cttggttcga gattcagttc cacggtgtgc
    15301 gcgccatgca gtttcgcttc gtgaacaaac ccagccgccg gataaacatg cccggaagta
    15361 ccaatggcaa tgaaaatatc agccatcgac aacgccatat aaatttcatc catgccgagt
    15421 ggcatttcgc caaaccaaac gacgtgcggg cgcaagggtg ccgggaactg gcaacaatgg
    15481 catttatctt ctggcgtaac gtctccggtc cagtcgagaa cctgaccact ttgcgaacaa
    15541 cgaactttca gcaattcccc atgcatatga atgacattgg tattacctgc gcgttcatgc
    15601 aggttgtcta tattctgcgt taccagcaaa aagcgatcgc cgagggcgtc ttgcagttta
    15661 gccagcgcaa gatgcgcggc gttaggctga atttctggct gctgcagctg tcgacgacgg
    15721 gcgttataaa acgcttgcac cagttcagga tcgcgatcga aaccttccgg cgttgccaca
    15781 tcttcaaccc gatgttcttc ccacaggcca tctgcggcgc gaaaggtacg aatacctgat
    15841 tccgcagaaa ttcctgcccc tgtcagtacg agtactcttg gtttttccat tgcttccggc
    15901 accactttat ctctgaaaaa aatacgctga cgcaaacgct cgcgcaggcg gcgtttattt
    15961 ttacgaaaac gacttaaccg atgaccccga cgcgacagca tagcaacctc tgttgtttaa
    16021 tcggttagat gtaggaaggc cgcaccacgc attcctcccg catcaccgtg gcgcgcgcgt
    16081 tcaatgcgcg gaacacgagc tacaggtaag agatgacgag gcagcctgtc cgccagttgc
    16141 gttgtgattg ccgggaaatt cgataagcca ccaccaatga cgaccaggtc agagtcaacg
    16201 atggtcagga tatttcccag acaaaccgct aataaatcca gataacgctc aacgtgcgcc
    16261 cttgcctgct catcgccttg atcataaagc gcaataattt cgggagcctg caacggttga
    16321 tgataatagt gttgccagag ccacgcaaag ccccgacctg atagataatt ttcgatacaa
    16381 ccaatttgac cacagccgca gcggcgtaac gggaaatcca gccccatcat ggttaacgca
    16441 tcaaccggca gacgcatatg gccaaactcg ccggtaatgt agcttttacc ggtaatcggt
    16501 ttaccgttga aaatcagccc gccgccaacg ccggtgccga gaattaaccc catcaccagt
    16561 ggatattgcg taaattcatc atcccaggct tctgaaaggg caaaacagtt ggcatcgtta
    16621 tcaaggcgta catcgcgatc aagacgtgcg ctcaggtcgg cacgcagcgg tttaccgctg
    16681 gcagcgggga cattggcggc atacagcgta ccatcttctg tttccggcat tcccggaata
    16741 ccgatgccaa cagaaccttt acagccaaaa cgttggtcag cttcggctac cagctcgcat
    16801 actgcatcta aaaatgcgtc atagctgtca cgcggtgtcg gcacccgctt ttcccactgc
    16861 aactgccgac cgctatcaaa cacgccaagc gcaatttttg ttccaccaat atcaaacccg
    16921 taatacattg ccgctccttg ttttaatgta ctgcctttac tggccgctaa ggactcgcgc
    16981 agggtcaata ttactggcgc gccgcgccgg ataccaactt gccaaaagac tcagcaacaa
    17041 tgctgtgacc agtacgtaga agacgtccag ccagtgcaat tccgatggca ggaagtcaat
    17101 aaaatagata tcgctggaga ggaactgatg accgatcagc ttttcaatcc gctcaataat
    17161 cggggtaagt tgcagtgaaa caactacgcc gataatcaca ccacacaggc taccgaacag
    17221 ccctgccagc aatccatacc agacaaagat ggcgcgaatt aaaccatctt tcgcccccag
    17281 cgttcttaat actgcgatat cgccactctt gtctttcacc gccatcacta aggtggagac
    17341 gatgttgaaa caggccacgc caatcaccag taccatcgcc agatacataa tggcgcggat
    17401 catctggata tcgcgataca tatagccgta agtaccaatc caacttttaa tataaacata
    17461 gctgttggtc acttcacccg catcgcgtac cagcttattg gcgttgaaaa catccgtcat
    17521 tttaagggca atacctgaca cgctggaacc catatcaaga tattgttggg catccgccag
    17581 cgggatcatg gcaaaactgt gatcgagttg accactcaac tgcaaaatac cggcaatgtg
    17641 caaacgcaca cgttttggct gcatcagttt atgctcggga ttcgagttgg gaatcatgat
    17701 cgacacccaa tcgccctgct tcactttcag cgcatccgcc acgcctttgc cgatgataat
    17761 ttgctgttcg cccgctttaa aattgcgcca ggcatccccc tgaacaaacg agggtaatgc
    17821 gctcagacgc tgttcctgtt gcgggttaac gcccttcacc tgaattgcgc gcagattggt
    17881 accactttct accagtccgg tgaaattgat atacggcgca gcggcggcaa tacccggcac
    17941 tttttgcacg ttatccagtg cttcctgcca gttagtccat ggctgattta ccgcctcaat
    18001 ttcaccgtgc ggtaccacag caagaatgcg gttattcagt tcgcgttcaa agccgttcat
    18061 cgcgcttaag ccgacgatca ataccgccac gccgagggca atgccaatgg tagaaatgac
    18121 agagattagc gacaccatgc cgccgcgtcg ccgtccgcga ctaaaacgca ggccaatcaa
    18181 taacgataaa ggcatcgcca ttactccgcc cccatcaggc tcagttccgc cgtcagacgt
    18241 ccatcacgca tttccagttg gcggctcata cgtttcgcca gttgcaggtc gtgagtaacc
    18301 accaggaagg cggtgccctg caagcgattc aattccccaa gcaactggaa gatgctgtcg
    18361 gcgttacgcg catcgaggtt accggtaggc tcatccgcaa gtaccagacg cggattattg
    18421 accagcgcac gggcaatcgc cacacgctgg cgttcgccgc cagaaagttc agatgggcgg
    18481 tgattcgcac gatgctccag ccccaccgct tttaacatct ccagcgcacg gctgttgatt
    18541 tcagcgggct ttttcttgcc aatcagcagt ggcatagcca cgttttccag ggcagtaaaa
    18601 tccggcagca ggtggtgaaa ctgataaata aagcccagct tctggttgcg cagttctgct
    18661 ttcgccgccg aagacagttt gctcatcggt tgaccgttaa agatcacatc gccggaggtt
    18721 ggtgtatcca gcccgcccag caggtgcagc aaggtacttt taccggaacc agagctaccg
    18781 acgatcgcca tcatttcacc ttcgccgacg ctgaaactga cattgtgcag tacatcggtt
    18841 tgcacactgc cttcctgata gcgtttgcac aggttgtcgc attgcaacag gatcttattc
    18901 ataacgtaaa gcctcagcgg gttgagtggc ggcagcgcgc catgaagggt aaagcgtaga
    18961 cagcagcgcg atagccatcg ccaccagcgc aataacaatg acctgtaaag gttcgatagc
    19021 gaccggcagc gccgcgccat caagcaggac gccgattatc ggcatcagat tatttaactg
    19081 gctggcaagc agtgcgccaa gcgccgctcc gaggatcgca ccgataatcc cggcgctggc
    19141 cccttgcacc ataaagacca tcatgatttg tcgcggagtt aagccttgcg tttgcaggat
    19201 cgcgacttcg ccctgcttct ccattaccat cagccctagt gaggtaataa tgttaaacgc
    19261 cgcaacggcg acaatcaggc tcagcagcaa gcccatcata tttttttcca tgcgtacggc
    19321 ctggaacagc tcgcctttac ggtcacgcca gtcctgccat ttgctgcctt caggcagttt
    19381 ttgctgactt aaagagtcaa ctttcagcgg ctcatccagc cacaaacgcc agccggtaat
    19441 attgcctgcc ggataacgca tcagacgcga ggcatcctca atattcacca gcatttcata
    19501 gccatcgact tcactgttgg cggcgaaatt gccaatcaca ttgaacaggc gctggcttgg
    19561 aatacgcccc atcggcgtga actggctggc agatggtacc atcacgcgga tttgatcacc
    19621 gcgattaacg cctagctgtg aggcaagttg ttcgccgagg atgacattat atttccccgg
    19681 ctcgaggtca gtttgtttca cattgaccag atacggcgta agtggatctt tttgcgccgg
    19741 gtcgataccg agcatcaccc cgaccgccac gctgcgcgcg ctttgcagta ccacatcacc
    19801 ggtagtaata ggtgcgacgc gattaacgcc gtccagtttg actgccgttt ccgggagttg
    19861 ctgcgggtta agagagccat gctcagaaga gagaattgcc tgtggcatca ggccaaggat
    19921 gttgttttgc agctcgcgct caaagccgtt catcactgac aatactgtga ccagcgccat
    19981 caccccgagg gtaatgccga tggtagaaag ccaggagacg aaacgaccga agcgatccgc
    20041 tgcacgccca cgcatgtaac gcaggccaat aaatagagcg acaggttggt acatgaaatc
    20101 cgtctggttg ctgtagcaaa gacacgagta tataagcgaa agcaagatgg gtaaacgtct
    20161 gaactgtaag tgatcgtcag ggctaatctg aaaagccccc gtaaatcaat cggctaaatt
    20221 gccatgtttt atcatcaatc ttgccgcctg atcttaatct gaaaaatcat ccaatacaga
    20281 cagataccca ttacacctcg ggtcataacc cgcaggatca accgcatgta aattaaaaaa
    20341 gagcgtgttg tcctgatgaa acaaaaagag ctatggatta accagatcaa agggttatgt
    20401 atttgtctgg tggtgattta tcactcggtc attacctttt atccgcatat gaccactttt
    20461 cagcatccgt tatcggaagt cctgagtaaa tgctggatct atttcaatct ttaccttgca
    20521 ccctttcgta tgccggtttt tttctttata tccggctatt tgattcgccg ctatatcgac
    20581 agcgtaccgt ggggaaattg tctcgataaa cggatctgga acatcttctg ggtgctggcg
    20641 ctttggggcg tggtgcagtg gctggcgtta agtgcgctga accagtggct ggcacctgag
    20701 cgcgatttaa gcaatgcctc caatgccgct tatgccgatt ccaccggtga gttcctgcac
    20761 gggatgatca ccgccagcac cagcttgtgg tatctgtatg cgttaattgt ctatttcgtg
    20821 gtatgtaaaa tttttagccg tctggcgctg ccactattcg ccttgtttgt actgctgagt
    20881 gtggcggtta atttcgttcc cacgccgtgg tggggaatga acagtgtgat ccgcaatttg
    20941 ccttattaca gccttggcgc atggtttggc gcaacaataa tgacctgtgt taaagaggtg
    21001 ccgttgcgcc gccatctgct gatggcttct ttgcttaccg ttctggcggt cggtgcctgg
    21061 ttgtttacta tctcgctgct gttgtcgctg gtatcgattg tggtgatcat gaagctgttt
    21121 tatcagtacg agcaacgatt cggtatgcgc tccaccagcc tgctgaatgt gattggctcc
    21181 aacaccattg ctatctacac cacccatcgc attctggttg aaatattcag cttaactctg
    21241 cttgcgcaaa tgaacgcagc acgctggtcg ccgcaagtcg aactgacact cctgctggtt
    21301 tatccctttg tcagtttgtt catctgtact gttgcgggct tgctggtaag aaaactttca
    21361 cagcgtgcat tcagcgatct gttgttctcc ccgccttcac tgcccgctgc cgtcagttac
    21421 tctcgttaaa ccatcgcccc agccggggcg attcaatttg ctaaaccatg tcgattacgg
    21481 ataatgaaca gcattgctta tcagagatat gcccccatat gttgaggcat atcctaacga
    21541 gaatctgaca accgttatgc ctgaacaata tcgttataca ctgcccgtca aagcgggtga
    21601 gcagcgtctg ctgggcgagt taaccggcgc agcctgtgca acgctggtag cggaaattgc
    21661 cgaacgtcac gccggtccgg tggtactcat tgcaccagat atgcaaaatg ctctgcgttt
    21721 gcatgatgaa atcagccagt tcaccgatca gatggtgatg aatctggcgg actgggaaac
    21781 tcttccctac gacagttttt cgcctcatca ggacattatc tcctcgcgcc tttccaccct
    21841 ttaccagcta ccgacgatgc agcgtggcgt actgattgtt ccggtgaata cgcttatgca
    21901 gcgcgtttgc ccacacagtt ttctccacgg tcatgcgctg gtgatgaaaa aaggtcagcg
    21961 cctgtcacga gatgcattac gaacccaact ggacagcgcc ggttatcgcc atgttgacca
    22021 ggtgatggag cacggcgaat acgccacgcg cggcgcgttg ctggatctct tcccgatggg
    22081 gagtgagctg ccttatcgtc ttgatttctt tgatgatgaa atcgacagcc tgcgggtgtt
    22141 tgacgtcgac agccagcgca cgctggagga agtagaagcg atcaatctgc tgcccgcgca
    22201 cgaatttccg accgataaag cggcaattga actgttccgc agccagtggc gcgatacctt
    22261 cgaagtgaag cgcgatccgg aacatattta ccagcaagtg agtaaaggca cattacctgc
    22321 cgggatcgag tactggcagc cattgttctt cagcgaacca ctgccgccgc tgttcagtta
    22381 tttccctgcc aataccttgc tcgtgaatac tggcgatctg gaaaccagtg ccgaacgttt
    22441 ccaggctgac acgctggcgc gttttgagaa tcgcggcgtc gatccgatgc gcccgctgtt
    22501 gccaccacaa tcgctctggc tgcgggtgga cgagctcttc tcagagctga aaaactggcc
    22561 ccgggtgcag ctaaaaactg aacatttacc gacaaaagcc gcgaatgcca atttaggttt
    22621 ccagaaactg ccagacctgg ccgttcaggc acaacaaaaa gcgccgctgg atgcgctgcg
    22681 taagttcctc gagactttcg acggtccggt ggtgttctcg gtagaaagtg aaggtcgccg
    22741 tgaagcgctg ggtgaactgc tcgcacgaat taaaattgct ccgcaacgca ttatgcgtct
    22801 tgatgaagcc agcgaccgtg ggcgttatct gatgattggc gctgccgaac atggttttgt
    22861 cgataaggtg cgtaatctgg cgctgatctg cgaaagcgat ctgctcggtg aacgtgttgc
    22921 ccgccgtcgt caggattctc gccgcaccat caaccccgat acactgatcc gtaaccttgc
    22981 ggagctgcat attggtcagc cggtggtcca tctggagcac ggtgtcgggc gctatgccgg
    23041 aatgaccacg ctcgaagcgg gcggcattac tggcgagtat ttgatgctca cctatgccaa
    23101 cgacgccaaa ctgtatgttc cggtgtcgtc actgcatctg attagccgtt acgcaggtgg
    23161 cgcggaagaa aacgccccgc tgcataaact tggcggcgat gcgtggtcac gcgcgcggca
    23221 gaaagcggcg gaaaaagtgc gtgatgtggc ggcggaattg ctggatatct acgcgcaacg
    23281 cgccgccaaa gagggcttcg cgtttaaaca cgatcgtgag cagtatcagt tgttctgcga
    23341 cagcttcccg tttgaaacca cgccggatca ggcgcaggcc attaatgcgg tacttagcga
    23401 catgtgtcag ccgctggcaa tggatcgtct ggtgtgcggc gatgttggct ttggtaaaac
    23461 agaagtggcg atgcgcgccg ctttcctggc agtagataac cacaagcagg tggcggtgtt
    23521 ggtgcctacc acccttctcg cgcagcagca ttacgacaac ttccgcgacc gtttcgccaa
    23581 ctggccggta cgtatcgaaa tgatctcccg tttccgcagc gccaaagagc agacgcaaat
    23641 ccttgcggaa gtggcggaag ggaaaatcga tattctgatc ggtacgcaca aactgctgca
    23701 aagtgacgtc aagtttaaag atttaggcct gctgattgtc gatgaagaac accgcttcgg
    23761 ggtgcgtcat aaagagcgca ttaaagcgat gcgcgcgaac gtggatattc tgacgcttac
    23821 tgcaacgccg atcccacgca cgctgaatat ggcaatgagc ggaatgcgtg acctgtcgat
    23881 tatcgccacg ccgcccgccc gtcgtctggc agttaaaacc tttgtccgtg agtatgacag
    23941 cctagtggtc cgggaggcga tcctgcgtga aattttgcgc ggggggcagg tttattatct
    24001 ctacaatgat gtggaaaaca tccagaaagc tgccgaacgg ctggcagaac tggtgccaga
    24061 agcgcggatc gccatcggtc acgggcagat gcgcgagcgc gaactggaac gggtgatgaa
    24121 tgatttccat catcaacgtt tcaacgtgct ggtttgtaca accattatcg aaaccgggat
    24181 cgacatcccg acagccaaca ccattatcat tgaacgcgcg gatcacttcg gtctggcgca
    24241 gctgcaccag ttacgcggtc gcgtcggacg ttcgcatcat caggcatatg catggttgct
    24301 gacaccgcat ccaaaagcga tgactaccga tgcacaaaaa cgtcttgaag cgattgcctc
    24361 gctggaagat ctcggggcag gttttgcgct ggcaacgcac gatctggaga ttcgcggcgc
    24421 gggtgaactg cttggcgaag aacaaagcgg ctcaatggaa accatcggtt tctcgctgta
    24481 tatggagttg ctggaaaacg ccgtcgatgc actgaaagcc ggacgcgagc cgtcgctgga
    24541 agatctcacc agccagcaaa cagaagtcga gctgcggatg ccgtcgctat tgccagatga
    24601 tttcattcct gacgtgaata cgcgtttgtc gttctataaa cgtattgcca gcgccaaaac
    24661 ggaaaacgaa ctggaagaga tcaaagtcga gcttatcgat cgcttcggcc tgctgccaga
    24721 tccggcgcgt accctgctgg atattgcccg tctgcgccag caagcgcaga aactggggat
    24781 caggaagctg gaaggtaatg agaaaggcgg ggtgatcgaa tttgccgaga agaatcacgt
    24841 taatccggcc tggttgattg gtttgctgca aaaacagccg cagcattacc gccttgatgg
    24901 tccgacgcgc ctgaaattta ttcaggattt gagtgagcgg aaaacgcgta tcgaatgggt
    24961 acgccagttt atgcgtgaac tggaagagaa cgcgatcgct taatggtaaa tcgggggcgt
    25021 ttctgcgccc ccatctgcaa catttacaaa ttctttgcac ttccctgcac tatccgacac
    25081 tgtcaccatc cataattcag gcttatctgt ttattacaat aaccttatat ttattattga
    25141 tttttggtga tgatcaaaac gcgtttttct cgctggctaa cgttttttac gttcgccgct
    25201 gccgtggcgc taccggcaaa agccaacacc tagccgctgc cgccagcgag cagtcgtctg
    25261 gttggcgaaa acaaatttca tgtggtggaa aatgacggtg gttctctgga agccatcgcc
    25321 aaaaaatata acgtcggctt tctcgctctg ttacaggcta accccggcgt tgatccttac
    25381 gtaccgcgcg cgggcagcgt gttaacgatc ccgttgcaaa ccctacttcc agacgcgccg
    25441 cgcgaaggca ttgtgatcaa cattgcggag ctgcgtctct attactaccc gccgggtaaa
    25501 aattcggtaa ccgtgtatcc aataggtatt ggtcagttag gtggtgacac gctgacaccg
    25561 acaatggtga ccaccgtttc agacaaacgt gcaaacccaa cctggacgcc aacggcaaac
    25621 atccgcgccc gttataaagc acagggaatt gagttgccta cggtagtgcc ggctggaccg
    25681 gataacccaa tgggccatca tgcgattcgt ctggcggcct atggcggcgt ttatttgctt
    25741 cacggtacga acgccgattt cggcattggc atgcgggtaa gttctggctg tattcgtctg
    25801 cgggatgacg atatcaaaac actctttagc caggtcaccc caggcaccaa agtgaatatc
    25861 atcaacactc cgataaaagt ctctgccgaa ccaaacggtg cgcgtctggt tgaagtacat
    25921 cagccgctgt cagagaagat tgatgacgat ccgcagctgc tgccaattac gctgaatagc
    25981 gcaatgcaat catttaaaga tgcagcacaa actgacgctg aagtgatgca acatgtgatg
    26041 gatgtccgtt ccgggatgcc ggtggatgtc cgccgtcatc aagtgagccc acaaactctg
    26101 taaatcctgg ataaaaaaaa tccccgcgac acggcatgtc gcggggaaaa aatcagcagt
    26161 agcaggcatt aatgagggtt aatgcttatt tataaattac tgctgttcca tggagggtat
    26221 tcggaccggt tacagaagta atacggaaag attttgcgcc catctcatcc gctttttgcg
    26281 ccagctgctc ttccagcgat cccagatttg tccccgcgtt agcactgata gtaccgactt
    26341 tttgttggcc ttctggcgtt gactgaactt cgacagccgc aaagttggca aatgacatgg
    26401 agcttaaaat cgccgcagcg atgagggttt ttacgttttt cataatagtg gccttatgca
    26461 gatgaatgac gtcgcaagaa attacttaac gatcgataac taaatgatag atgtgatctg
    26521 gatcaca
//
LOCUS       DAZPAC010000042        25774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZPAC010000042 DAZPAC010000000
VERSION     DAZPAC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.042"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(159..371)
                     /gene="rpmE"
                     /locus_tag="ACHUPM_001419"
     CDS             complement(159..371)
                     /gene="rpmE"
                     /locus_tag="ACHUPM_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR0869244.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            574..2772
                     /gene="priA"
                     /locus_tag="ACHUPM_001420"
     CDS             574..2772
                     /gene="priA"
                     /locus_tag="ACHUPM_001420"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR0869245.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2928..3953
                     /gene="cytR"
                     /locus_tag="ACHUPM_001421"
     CDS             2928..3953
                     /gene="cytR"
                     /locus_tag="ACHUPM_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR0869246.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4045..5004
                     /gene="ftsN"
                     /locus_tag="ACHUPM_001422"
     CDS             4045..5004
                     /gene="ftsN"
                     /locus_tag="ACHUPM_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR0869247.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5097..5627
                     /gene="hslV"
                     /locus_tag="ACHUPM_001423"
     CDS             5097..5627
                     /gene="hslV"
                     /locus_tag="ACHUPM_001423"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR0869248.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5637..6968
                     /gene="hslU"
                     /locus_tag="ACHUPM_001424"
     CDS             5637..6968
                     /gene="hslU"
                     /locus_tag="ACHUPM_001424"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR0869249.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7035..7961
                     /gene="menA"
                     /locus_tag="ACHUPM_001425"
     CDS             7035..7961
                     /gene="menA"
                     /locus_tag="ACHUPM_001425"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR0869250.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8054..8539
                     /gene="rraA"
                     /locus_tag="ACHUPM_001426"
     CDS             8054..8539
                     /gene="rraA"
                     /locus_tag="ACHUPM_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR0869251.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8624..8869)
                     /gene="zapB"
                     /locus_tag="ACHUPM_001427"
     CDS             complement(8624..8869)
                     /gene="zapB"
                     /locus_tag="ACHUPM_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR0869252.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9295..10140
                     /gene="glpF"
                     /locus_tag="ACHUPM_001428"
     CDS             9295..10140
                     /gene="glpF"
                     /locus_tag="ACHUPM_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFR0869253.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10163..11674
                     /gene="glpK"
                     /locus_tag="ACHUPM_001429"
     CDS             10163..11674
                     /gene="glpK"
                     /locus_tag="ACHUPM_001429"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR0869254.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11804..12814
                     /gene="glpX"
                     /locus_tag="ACHUPM_001430"
     CDS             11804..12814
                     /gene="glpX"
                     /locus_tag="ACHUPM_001430"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR0869255.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12911..13657
                     /gene="fpr"
                     /locus_tag="ACHUPM_001431"
     CDS             12911..13657
                     /gene="fpr"
                     /locus_tag="ACHUPM_001431"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR0869256.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13662..14090)
                     /gene="uspD"
                     /locus_tag="ACHUPM_001432"
     CDS             complement(13662..14090)
                     /gene="uspD"
                     /locus_tag="ACHUPM_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR0869257.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14117..14416)
                     /locus_tag="ACHUPM_001433"
     CDS             complement(14117..14416)
                     /locus_tag="ACHUPM_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR0869258.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14628..15068)
                     /locus_tag="ACHUPM_001434"
     CDS             complement(14628..15068)
                     /locus_tag="ACHUPM_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0869259.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15169..15768
                     /locus_tag="ACHUPM_001435"
     CDS             15169..15768
                     /locus_tag="ACHUPM_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR0869260.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            15876..16643
                     /gene="tpiA"
                     /locus_tag="ACHUPM_001436"
     CDS             15876..16643
                     /gene="tpiA"
                     /locus_tag="ACHUPM_001436"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR0869261.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16698..17453)
                     /gene="cdh"
                     /locus_tag="ACHUPM_001437"
     CDS             complement(16698..17453)
                     /gene="cdh"
                     /locus_tag="ACHUPM_001437"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR0869262.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17560..18549)
                     /gene="sbp"
                     /locus_tag="ACHUPM_001438"
     CDS             complement(17560..18549)
                     /gene="sbp"
                     /locus_tag="ACHUPM_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR0869263.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18869..19831)
                     /gene="pfkA"
                     /locus_tag="ACHUPM_001439"
     CDS             complement(18869..19831)
                     /gene="pfkA"
                     /locus_tag="ACHUPM_001439"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR0869264.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20012..20914)
                     /gene="fieF"
                     /locus_tag="ACHUPM_001440"
     CDS             complement(20012..20914)
                     /gene="fieF"
                     /locus_tag="ACHUPM_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR0869265.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21062..21562)
                     /gene="cpxP"
                     /locus_tag="ACHUPM_001441"
     CDS             complement(21062..21562)
                     /gene="cpxP"
                     /locus_tag="ACHUPM_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR0869266.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21712..22410
                     /gene="cpxR"
                     /locus_tag="ACHUPM_001442"
     CDS             21712..22410
                     /gene="cpxR"
                     /locus_tag="ACHUPM_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR0869267.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22407..23780
                     /gene="cpxA"
                     /locus_tag="ACHUPM_001443"
     CDS             22407..23780
                     /gene="cpxA"
                     /locus_tag="ACHUPM_001443"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR0869268.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23886..24560)
                     /gene="yiiM"
                     /locus_tag="ACHUPM_001444"
     CDS             complement(23886..24560)
                     /gene="yiiM"
                     /locus_tag="ACHUPM_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR0869269.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24709..25692)
                     /gene="kdgT"
                     /locus_tag="ACHUPM_001445"
     CDS             complement(24709..25692)
                     /gene="kdgT"
                     /locus_tag="ACHUPM_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFR0869270.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
BASE COUNT         6364 a         6712 c         6638 g         6060 t
ORIGIN      
        1 gtaaaatcct tttgaaataa ataagaacca gaagcctagc gccagtcaac aacaggaaat
       61 gataatgagt taacaccccg gaatttttca gaatttataa aaggcaaatt acaggcacaa
      121 aaaaagcgcc gtgcggcgct tttttcggaa atccggtctt atttgctgcc cgggatgttg
      181 aaacgcttgt tgaagcggtc aacacggcca ccggtagcaa catcacgctg tttgccagtg
      241 aagaacgggt ggcacttgct gcacacgtcg aggttcaggt catgaccaac ggtggagcgg
      301 attttcatta cgttaccgca agagcagcta gcagtaattt cttcgtattt cgggtgaata
      361 tcttttttca tgggaaaacc tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag
      421 acaccacgcg atgttaaaag tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat
      481 catacagaaa ttaaccagcg tatgcaaact gatccgcact cttctacggc aatgtgtata
      541 ctaacccacc gaatttcaag tcaggatgat gctatgcccg ttgcccacgt tgccttgccc
      601 gttccgcttc ctcgtacctt tgactatctg ctgccagaag gcatggcggt taaagccggg
      661 tgtcgcgtgc gcgtgccgtt tggcaaacag caggagcgca tcggggttgt ggtatcagtt
      721 agcgatgtca gtgagctgcc gcttaacgag ctgaaagcgg tagttgaagt gctggacgtt
      781 gagccggtat ttactcactc cgtctggcga ttactgctct gggcggcaga ttactatcat
      841 catccgattg gcgatgtgct gtttcatgcc ttgccgattt tactgcgcca ggggcggcct
      901 gcggcgaacg cgccgatgtg gtactggttt gccactgaac aaggccaggc ggtggatctg
      961 aacagcctga aacgctcacc caagcaacaa caggcgctgg cggcgttacg gcaagggaaa
     1021 atctggcgcg accaggttgc cacgctcgaa tttaatgatg ccgcgttgca gacgctgcgc
     1081 aaaaaaggtc tgtgtgattt agcaagtgaa acaccagagt ttagcgactg gcgaacgaac
     1141 tatgccgttt ctggtgagcg gttgcgattg aataccgaac aggccaccgc cgttggcgca
     1201 attcatagcg cggcagatac tttttctgcc tggctgctgg cgggcgttac cggttccggt
     1261 aaaacggagg tttatctcag tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg
     1321 gtgatggtgc cggaaatcgg cctgacaccg caaactatcg cccgttttcg tgaacggttt
     1381 aatgcgcccg tggaagttct gcattccggt ctgaacgaca gcgagcgtct ttcggcgtgg
     1441 ctgaaagcga aaaatggcga ggcggcgatt gtgatcggca cccgctccgc gctgtttacg
     1501 ccgtttaaaa atctcggcgt gattgtcatt gacgaagagc acgacagttc ctacaagcag
     1561 caggaaggct ggcgctatca tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa
     1621 atcccgatta ttcttggctc cgcaacgccc gcgctggaaa cgttgtgcaa cgtccagcag
     1681 aaaaaatacc gcctgctgcg cctgacgcgc cgggcgggga atgcgcgtcc ggcaattcaa
     1741 cacgtgctgg atttaaaagg tcagaaggtg caggcaggtc tggctccggc gttaatcacc
     1801 cgtatgcgcc agcatttaca ggccaacaat caggttatcc tctttcttaa ccgccgtggc
     1861 tttgcgcctg cgctactgtg ccacgactgc ggctggattg ccgaatgccc acgttgcgat
     1921 cactactaca cgctgcatca ggcgcaacag catttgcgct gtcaccactg tgacagtcag
     1981 cgtccggtgc cgcgccagtg cccttcctgc ggttccacgc acctggtacc cgtggggctg
     2041 ggcaccgaac agcttgaaca gacgcttgcg ccgctgttcc ctgatgtgcc catttctcgt
     2101 atcgaccgcg ataccaccag ccgcaaaggg gcgctggaac agcaactggc ggaagtacat
     2161 cgcggcggtg cgaggatttt gatcggcaca caaatgctgg cgaaaggtca ccactttccg
     2221 gatgtgacgc tggtggcgtt actggatgtg gacggcgcgc tgttttctgc cgattttcgc
     2281 tcggcagagc gtttcgctca gctttacacc caggtcgccg gtcgtgccgg gcgtgcgggt
     2341 aaacagggcg aagtggtgct gcaaacgcac catccggaac atcctctgtt gcaaacgttg
     2401 ctctataaag gctacgacgc ctttgccgaa caggcgctgg ctgagcgacg catgatgcag
     2461 ctgccgccgt ggaccagcca tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg
     2521 ccgctgttcc tgcaacaact gcgtaatctg atcctctcca gcccgctggc agacgacaaa
     2581 ttgtgggttc tcggtccggt tccggcactg gcacctaaac gtggcggtcg ctggcgctgg
     2641 cagatattgt tgcagcaccc ttcccgcgtg cgcttgcaac acatcattaa cggtacgctg
     2701 gcactcatca atacaatacc ggattcccgt aaggtgaaat gggtgctgga tgttgatccg
     2761 attgagggtt aaaccgctca cgatgcgagg cggatcgaaa aattcaatat tcatcacact
     2821 tttcatgaaa attctgtaac cgttttcacg cgctatctgc taaaaatgtt gccgatgtga
     2881 agtaaacatg gatgtagtac gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga
     2941 agcaggaaac tgccgcgacc atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga
     3001 ctgtctcccg agcattaatg aatcccgata aagtctccca ggccacccgt aatcgggttg
     3061 aaaaagcggc ccgggaagtg ggttatttac cgcagcctat ggggcgcaac gtcaagcgta
     3121 atgaatcccg caccattctg gtgattgtcc cggatatctg cgatcccttc tttagcgaaa
     3181 ttattcgcgg tatcgaagtt acggcggcaa atcacggata tctggtgctg attggcgact
     3241 gtgcgcatca aaatcagcag gaaaaaacct ttatcgattt gatcatcacc aagcaaattg
     3301 atggcatgtt gctgctgggt tcaaggctgc cgtttgatgc cagcattgag gaacagcgta
     3361 atctgccgcc gatggtgatg gcgaacgaat tcgcaccgga actggagcta cctacagttc
     3421 atatcgacaa tctgaccgcc gcatttgatg cagtaaatta tctatatgag caagggcata
     3481 aacggattgg ctgtatagcc ggtcccgaag agatgccgct gtgtcactac cgcctgcaag
     3541 gttacgttca ggcgctgcgt cgctgcggca ttatggttga tccgcaatac atcgcccgtg
     3601 gcgacttcac cttcgaagcc ggaagcaaag cgatgcagca gctgcttgat cttccacaac
     3661 cgcctactgc tgtcttctgc catagcgatg tgatggcgct cggcgcactt tctcaggcaa
     3721 aacgccaggg gctgaaagtc ccggaagacc tttccataat cggttttgat aacatcgacc
     3781 tgacgcaatt ttgtgatccg ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc
     3841 gggaagctat gctgttattg cttgatcaaa tgcaggggca acacgttggc agtggctctc
     3901 gtttaatgga ctgcgaactt atcatccggg gatcaacacg cgcgttacct taaagtaaac
     3961 ctttaagact gccgtgactg gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga
     4021 acgaataaat acagcgaaac gatagtggca caacgagatt atgtacgccg cagccaaccg
     4081 gcaccttcgc ggcgaaaaaa gagcacctca cggaaaaagc aacgaaatct gcctgcggtt
     4141 tctcccgcta tggtcgctat tgctgccgcc gtgcttgtga cctttatcgg tggtctgtac
     4201 ttcattacgc atcacaagaa agaagagtcc gagacgctgc aaagccagaa agtgaccggt
     4261 aacggactac caccaaaacc agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc
     4321 cagccgggtg tgcgtgcgcc cacagaacct tctgccggtg gtgaagtgaa aacgccggag
     4381 caactgacac cagaacaacg tcagcttctt gaacaaatgc aggctgatat gcgccagcag
     4441 ccaacgcagc tggttgaagt tccgtggaat gaacagacgc cggaacagcg tcagcaaacg
     4501 ctacaacgcc agcgtcaggc gcagcaatta gcggagcagc aacgcctggc tcagcagtcg
     4561 cgtacgactg aacaaagctg gcagcagcag acgcgtacgt cgcaagccgc gccggtacag
     4621 gcgcaaccgc gccagtctaa accggcttct actcagcagc cgtaccagga tctgctgcaa
     4681 acccctgcgc acacgaccgc gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc
     4741 gctgacgcgc caaaaccgac ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc
     4801 ggttcgttca gaggcgcgga acaggcagag acggtacgtg ctcagctggc gttcgaagga
     4861 tttgactcga aaatcaccac caacaatggc tggaatcgtg tggtcattgg cccggtgaaa
     4921 ggcaaagaga acgcagacag caccctcaat cggttgaaga tggcgggtca tacaaactgc
     4981 attcggctcg ccgccggggg ttgaaaccct caaaatcccc cccatctata attgcattat
     5041 gccccgtact tttgtacggg gtttgtactc tgtattcgta accaaggggt cagctcgtga
     5101 caactatagt aagcgtacgc cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca
     5161 cgttgggcaa taccgtaatg aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca
     5221 aagtcatcgc gggctttgcg ggcggtactg cggatgcttt tacgctgttc gaactgtttg
     5281 aacgtaaact ggaaatgcat cagggccatc tggtcaaagc cgccgttgag ctggcaaaag
     5341 actggcgtac cgatcgcatg ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa
     5401 ctgcatcgct tatcatcacc ggtaacggtg acgtggtgca gccagaaaac gatcttattg
     5461 ctatcggctc cggcggccct tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg
     5521 aacttagcgc ccgtgaaatt gctgaaaagg cgttggatat tgcaggcgac atttgcatct
     5581 ataccaacca tttccacacc atcgaagaat taagctacaa agcgtaagga tctcccatgt
     5641 ctgaaatgac cccacgcgaa atcgtcagcg aactggataa gcacatcatc ggccaggaca
     5701 acgccaagcg ttctgtggcg attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg
     5761 aagagctgcg ccatgaagtg accccgaaaa atatcctgat gatcggcccg accggtgtcg
     5821 gtaaaactga aatcgcccgt cgtctggcta agctggcgaa tgcgccgttc atcaaagttg
     5881 aagcgaccaa attcaccgaa gtgggctatg tcggtaagga agtggattct attattcgcg
     5941 atctgaccga tgccgccgtg aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc
     6001 gcgctgaaga actggcagaa gaacgtattc tcgacgtgct gatcccacct gctaaaaaca
     6061 actggggaca gaccgaacag cagcaggaac cgtccgctgc tcgtcaggca ttccgcaaaa
     6121 aactgcgtga aggccagctt gatgacaaag aaatcgagat cgatcttgcc gcagcaccga
     6181 tgggcgttga aattatggct cctccaggca tggaagagat gaccagccag ctgcagtcca
     6241 tgttccagaa cctgggcggc cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca
     6301 tgaagctgct gattgaagaa gaagcggcga aactggtgaa cccggaagag ctgaagcaag
     6361 acgctatcga cgctgttgag cagcacggga tcgtgtttat cgacgaaatc gacaaaatct
     6421 gtaagcgcgg cgagtcttcc ggtccggatg tttctcgtga aggcgttcag cgtgacctgc
     6481 tgccgctggt agaaggttgc accgtttcca ccaaacacgg gatggtcaaa actgaccaca
     6541 ttctgtttat cgcttctggc gcgttccaga ttgcgaaacc gtctgacctg atcccggaac
     6601 tgcaaggtcg gctgccaatc cgcgttgaac tgcaggcact gaccaccagc gacttcgagc
     6661 gtattctgac cgagccgaat gcctctatca ccgtgcagta caaagcactg atggcgactg
     6721 aaggcgtaaa tatcgagttt accgactccg gtattaagcg catcgcggaa gcggcatggc
     6781 aggtgaacga atctaccgaa aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt
     6841 taatggaaga gatttcctac gacgccagcg atttaagcgg tcaaaatatc actattgacg
     6901 cagattatgt gagcaaacat ctggatgcgt tggtggcaga tgaagatctg agccgtttta
     6961 tcctataatc gcgttcaatc attttcatca ttgtttgatg gggctgaaag gccccatttt
     7021 tattggcgcg tattatgact gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt
     7081 tacgacctaa aaccctcccc ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat
     7141 ggtggcaagg tcacttcgat ccgctggtcg ccctgctggt actaattacc gccgggctat
     7201 tacagatcct ttctaacctc gccaatgatt acggcgatgc ggtaaaaggc agcgataaac
     7261 ctgaccgcat tgggccgcta cgcggcatgc aaaaaggggt cattacccag caagagatga
     7321 aacgggcgct cattattacc gtcgtgctca tctgtctctc cgggctggca ctggttgcag
     7381 tggcatgcca tacgctggcc gattttgtcg gtttcctgat tcttggcggg ttgtcgatca
     7441 ttgccgctat cacctacacc gtgggcaatc gtccttatgg ttatatcggt ctgggtgata
     7501 tttccgtact ggttttcttt ggctggttga gcgtcatggg gagctggtat ttacaggctc
     7561 atacattgat tccggcactg atccttccgg cgaccgcgtg tggcctgctg gcaacggcag
     7621 tactgaacat taataacctg cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc
     7681 tggtggtgcg cttaggtgaa gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct
     7741 ctctggtatg tctggcgctg tttaatctct tttcgctgca tagcctgtgg ggctggctgt
     7801 tcctgctggc ggcaccatta ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc
     7861 cggtggcgat gcgaccaatg ctggaacgta ctgtcaaggg agcgttactg actaacctgc
     7921 tgtttgtttt agggatattc ctaagccagt gggcagcata actgacaaat atcaattaac
     7981 aattgatgat tttgccaaca gcctacatag cgcgatatac tgaaaattct cgcagcaact
     8041 gaatgttaag cctatgaaat acgatacttc cgagctttgt gacatctatc aagaagatgt
     8101 taacgtcgtg gaaccgctgt tctccaactt tggcggacgg gcgtcgtttg gcggacaaat
     8161 aatcacggta aaatgtttcg aggacaacgg gttgctgtac gatctgctcg aacagaatgg
     8221 ccgtggtcgt gttcttgtcg tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc
     8281 tgaactggcg cgtctggcag tacaaaatga atgggaaggt ctggttattt acggcgcggt
     8341 gcgtcaggta gatgacctag aagagttgga tatcggcatc caggcgatgg cggcaattcc
     8401 ggttggtgcc gctggcgaag gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt
     8461 aaccttcttc tccggcgacc atctttatgc cgacaatacc gggattattc tttcagaaga
     8521 tccgctggat attgaatgat aagaaaggca ccgcaaggtg ccttttttct gcgttacctg
     8581 ttggcctaca cagtaaagaa attacgcgaa agatgaagcg taatcagacc tcttccatgc
     8641 gacccagcag ggcctgcaga cgttcctgcc agccgttctg ctgttctttc agatggttgt
     8701 tctcacgctc cagctcttcg cgctgatgct gggcattttg aacttcctgc gacagtgagt
     8761 tgtttttttc tttcagctct tcgatttcca tctgcaacag agtgatggta tcaatcgcct
     8821 gctgtacttt tgcttccagt ttctcaaaca cttctaatga cattgtcata cctctcctga
     8881 attgcaaggc gttgatggat aaaaaatcct cgtcccgatt accggtgacg ccttaataaa
     8941 tacgagcgca ctttagttag ctccgattgt atgaagccgc gccatcgctg tccagcggca
     9001 cgccttgcag attacggttt gccacacttt tcatccttct cctggtgaca taatccacat
     9061 caatcgaaaa tgttaataaa tttgttgcgc gaatgatcta acaaacatgc atcatgtaca
     9121 atcagatgga ataaattgcg cgataacgct cattttatga cgaagcacac acattttaag
     9181 ttcgatattt ctcgtttttg ctcgttaacg ataagtttac agcatgccta caagcatcgt
     9241 ggaggtccgt gactttcacg catacaacaa acattaactc ttcaggatcc gattatgagt
     9301 caaacatcaa ccttgaaagg ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt
     9361 tttttcggtg tgggttgcgt tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg
     9421 gaaatcagtg tcatttgggg actgggggtg gcaatggcca tctacctgac cgcaggggtt
     9481 tccggcgcgc atcttaatcc cgctgttacc attgcattgt ggctgtttgc ctgtttcgac
     9541 aagcgcaaag ttattccttt tatcgtttca caagttgccg gcgctttctg tgctgcggct
     9601 ttagtttacg ggctttacta caatttattt ttcgacttcg agcagactca tcacattgtt
     9661 cgcggcagtg ttgaaagtgt tgatctggca ggcactttct ctacttaccc taatcctcat
     9721 atcaattttg tgcaggcttt cgcagttgag atggtgatta ccgctattct gatggggctg
     9781 atcctggcgt taacggacga tggcaacggt gtaccacgcg gccctttggc tcccttgctg
     9841 attggtctac tgattgcggt cattggcgca tctatgggcc cattgacggg ttttgccatg
     9901 aacccagcgc gtgacttcgg tccgaaagtc tttgcctggc tggcgggctg gggcaatgtc
     9961 gcctttaccg gcggcagaga cattccttac ttcctggtgc cgctttttgg ccctatcgtt
    10021 ggcgcgattg taggtgcatt tgcctaccgc aaactgattg gtcgccattt gccttgcgat
    10081 atctgtgttg tggaagaaaa ggagaccaca actccttcag aacaaaaagc ttcgctgtaa
    10141 tatgactacg ggacaattaa acatgactga aaaaaaatat atcgttgcgc tcgaccaggg
    10201 caccaccagc tcccgcgcgg tcgtaatgga tcacgatgcc aatatcatta gcgtgtcgca
    10261 gcgcgaattt gagcaaatct acccaaaacc aggctgggta gaacacgacc caatggaaat
    10321 ctgggccacc caaagctcca cgctggtaga agtgctggcg aaagccgata tcagttccga
    10381 tcaaattgca gctatcggta ttacgaacca gcgtgaaacc actattgtct gggaaaaaga
    10441 aaccggcaag cctatctata acgccattgt ctggcagtgc cgtcgtaccg cagaaatctg
    10501 cgagcattta aaacgtgacg gtttagaaga ttatatccgc agcaataccg gtctggtgat
    10561 tgacccgtac ttttctggca ccaaagtgaa gtggatcctc gaccatgtgg aaggctctcg
    10621 cgagcgtgcg cgtcgtggtg aattgctgtt tggtacggtt gatacgtggc ttatctggaa
    10681 aatgactcag ggccgtgtcc atgtgaccga ttacaccaac gcctctcgta ccatgttgtt
    10741 caacatccat accctggact gggacgacaa aatgctggaa gtgctggata ttccgcgcga
    10801 gatgctgcca gaagtgcgtc gttcttccga agtgtacggt cagactaaca ttggcggcaa
    10861 aggcggcacg cgtattccaa tctccgggat cgccggtgac cagcaggccg cgctgtttgg
    10921 tcagttgtgc gtgaaagaag ggatggcgaa gaacacctat ggcactggct gctttatgct
    10981 gatgaacacc ggcgagaaag cggtgaaatc agaaaacggc ctgctgacca ccatcgcctg
    11041 cggcccgact ggcgaagtga actatgcgct ggaaggtgcg gtgtttatgg caggcgcatc
    11101 cattcagtgg ctgcgcgatg agatgaagtt gattaacgac gcctacgatt ccgaatattt
    11161 cgccaccaaa gtgcaaaaca ccaatggtgt gtatgtggtt ccggccttta ccgggctggg
    11221 tgcgccgtac tgggacccgt atgcgcgcgg ggcgattttc ggtctgactc gtggggtgaa
    11281 cgctaaccac attatacgcg cgacgctgga gtctattgct tatcagacgc gtgacgtgct
    11341 ggaagcgatg caggccgact ctggtatccg tctgcacgcc ctgcgcgtgg atggtggcgc
    11401 agtagcaaac aatttcctga tgcagttcca gtccgatatt ctcggcaccc gcgttgagcg
    11461 cccggaagtt cgcgaagtga ctgcattggg cgcggcctat cttgctggtc tggcggtcgg
    11521 tttctggcag aacctcgacg agctgcaaga gaaagcggtg attgagcgcg agttccgtcc
    11581 aggcatcgaa accactgagc gtaattaccg ttacgcaggc tggaaaaaag cggttaaacg
    11641 cgcgatggcg tgggaagaac acgacgtaat gtaaatgccg gatgaagcgt ttatgccgca
    11701 tccggtagtc ccgaaacgtg cgggggcaac cccgcacaca tcaataatcc ctcccttccc
    11761 ctgtgctaca cttcgcgcca ttccttactg cttagagttt gctatgagac gagaacttgc
    11821 catcgaattt tcccgcgtca ccgaatctgc ggcgctggct ggctacaaat ggttaggacg
    11881 cggcgataaa aacaccgcgg acggcgcggc ggtaaacgcc atgcgtatta tgctcaacca
    11941 ggtcaatatt gacggcacca tcgtcattgg tgaaggtgaa atcgacgaag caccgatgct
    12001 ctacattggt gaaaaagtcg gtactggtcg cggcgacgcg gtagatattg ctgttgatcc
    12061 gattgaaggc acgcgcatga cggcgatggg ccaggctaac gcgctggcgg tgctggcagt
    12121 aggcgataaa ggctgcttcc tcaatgcgcc ggatatgtat atggagaagc tgatcgtcgg
    12181 accgggagcc aaaggcacca ttgatttgaa cctgccgctg gcggataacc tgcgcaatgt
    12241 tgcggcggcg ctcggcaaac cgttgagcga actgacggta acaattctgg ctaaaccacg
    12301 ccacgatgcc gttatcgctg aaatgcaaca actcggcgta cgcgtatttg ctattccgga
    12361 tggcgacgtt gcggcctcaa ttctcacctg tatgccagac agcgaagttg acgtgctgta
    12421 cggtattggt ggcgcgccgg aaggcgtagt ttctgcggcg gtgatccgcg cattagatgg
    12481 cgacatgaac ggtcgtctgc tggcgcgtca tgacgtcaaa ggcgacaacg aagagaatcg
    12541 tcgcattggc gagcaggagc tggcacgctg caaagcgatg ggcatcgaag ccggtaaagt
    12601 attgcgcctg ggcgatatgg cgcgcagcga taacgtcatc ttctctgcca ccggtattac
    12661 caaaggcgat ctgctggaag gcattagccg caaaggcaat atcgcgacta ccgaaacgct
    12721 gctgatccgc ggcaagtcac gcaccattcg ccgcattcag tctatccact atctggatcg
    12781 caaggacccg gaaatgcagg tgcacatcct ctgattgatt tgatcgattg agccttccag
    12841 tccttcggga ctggaatttt tttgttcgga gaacgaagat aaggcaagtc aatcaaaaca
    12901 ggagaaaaac atggctgatt gggtaacagg caaagtcact aaagtgcaga actggaccga
    12961 cgccctgttt agtctcaccg ttcacgcccc cgtgcttccg tttaccgccg ggcaattcac
    13021 caagcttggc cttgaaatcg acggcgaacg cgtccagcgc gcctactcct atgttaactc
    13081 gcccgataat cccgatctgg agttttacct ggtcaccgtc cccgatggca aattaagccc
    13141 acgactggcg gcactgaaac caggcgatga agtgcaggtg gttagcgaag cagctggctt
    13201 ctttgttctg gaggaagtac cagattgcga aacgctatgg atgctggcaa ccggtacagc
    13261 gattggccct tatttatcga ttctgcaact aggcaaagat ttagatcgct tcaaaaatct
    13321 ggtcctggtt cacgccgcac gttatgccgc cgacttaagc tatttgccac tgatgcaaga
    13381 actggaaaaa cgctacgaag gcaaactgcg cattcagacg gtggtcagtc gggaaacggc
    13441 agcggggtcg ctcaccggac ggataccggc attaattgaa agtggggaac tggaaagcgc
    13501 gattggcctg ccgatgaata aagaaaccag ccatgtgatg ctgtgcggca atccacagat
    13561 ggtgcgcgat acacaacagt tgctgaaaga gacccggcag atgacgaaac atttacgtcg
    13621 ccgaccgggc catatgacag cggagcatta ctggtaagcg gttacttatc gataaacggc
    13681 acgatgagca aatccgcact catcttattg atcatcccgc gatatgccgg catcaaacgg
    13741 ttgataaatg agtggtgatg accacagaca aggaggtcgc actgctcttt ttgcataatt
    13801 tccagcagtg tttccggcat ttctccgcgt tcaatacgca gttttgtctt cggccattga
    13861 atatttttcg tcagtttata cagcttgtta tccgacttat tcttcaacaa ttgaagaata
    13921 tcttctgttg cagggaagta gatacccgga tacaactcgc ttaagccatc atcaatatga
    13981 attaacgtca ggtgagcgtc attatgtctg gcgagctcca gggctttatt caccagtaag
    14041 gcatcttctt cattcccgga aattgccacg ccaatgtgtt tataagccat gtttaactcc
    14101 ttctaaagca actccatcaa gctaacgaac gcagtgataa ctcaaaaata atcatctcgg
    14161 cctggcatga gaaagtgaag gccgcatcaa gctcaacttg cccctccaga tccttaatct
    14221 tataggccac gctggcccct tcatctgcag aggatgcagc ggcggcaagc tctttcagtt
    14281 tggtcataaa agactctgct tcttcgcggg tggcaaaaaa gcgcgaaaac ttactggtac
    14341 agttgtcgtt atcaatcacc gtaccgatat caatcgcaca tcctttagta ctgcatttat
    14401 ctacgacatc tttcataggg cacctctgta tattcgcctc tctgtcgatt acgacttaag
    14461 aataatgcgc cgttaaggga agaacaaaca agtaatgatc ttatggatta ccattttttt
    14521 tgatattgct taaggtgaag agttcagaag gattttccgg gtattgtcat ttgcatcgtg
    14581 gaaataacaa tcccggaaaa aaatagcagt aagagatata ttaaaggtta attacttgat
    14641 ttattgtcgg ctttatactt cacatcctga gtatctttac catatttatt ttcgccttgt
    14701 gtgccaacaa acgcaccaag atcgataagc atcatcacca gaatcaacgt cgggacaaaa
    14761 cgccccaccg cccattgcca gacacccggt aaaatcgccc agttacccgc cagcagcatc
    14821 cacgccacaa tcatcagaaa tgcccatgcg ccggaacgcc cgcgatcatg caagcgctta
    14881 acagttactg ccgctgttgg ccaaagcaag cacacaaggc aaaacgccgc ggtctgaata
    14941 tcgagtaaat ttttacccgc cagtgagaaa agcaccagca tgcctgcgaa ccacaggcct
    15001 atccaaatcc agaaatcacg gcgtccaata cgccctttaa atgagaataa ccattgctgt
    15061 atggtcatgt aagttccttg atggttgtct tttccaggat tctacccttt tgacaagggc
    15121 gacagatatc gttttaatcg gagccagtca taacaaaagg tactgtcaat gaagccaggg
    15181 tgtacgctgt tttttctctt atgttctgca ttaaccgtta caacaacggc gcatgcacaa
    15241 acaccagata cggcaacgac cgcgccttat ctgctggctg gagcccctac tttcgatctc
    15301 tccatcagcc agtttcgaga agactttaac agccagaatc ctagcctgcc actgaacgaa
    15361 tttcgtgcca tcgacagcag tcccgacaaa gccaatctca ctcgtgctgc aagtaaaatt
    15421 aacgagaact tgtatgcttc tacagcgctg gagcgcggta ccttaaaaat caaaagcatt
    15481 caaatgacct ggctacccat ccaggggcca gagcaaaaag cagcgaaagc gaaagctcag
    15541 gaatacatgg cagcagtgat ccgcacactc accccattaa tgaccaaaac acaaagccag
    15601 aaaaaactgc agtcgctact aacggcgggg aaaaacaaac gttattacac cgagacagaa
    15661 ggtgcactgc gttatgttgt cgcggacaac agcgaaaagg ggctgacctt cgctgttgaa
    15721 ccgattaagc tggcgctatc tgaatcgctt gaaggtttga ataaatgaca aaaagcaaag
    15781 cctttgtgcc gatgaatctc tatactgttt cacagacctg ctgccctgcg gggcggccat
    15841 cttcctttat tcgcttataa gcgtggagaa ttaaaatgcg acatccttta gtgatgggta
    15901 actggaaact gaacggcagc cgccacatgg ttcacgagct ggtttctaac ctgcgtaaag
    15961 agctggcagg tgttgctggc tgtgcggttg caatcgcacc accggaaatg tacatcgata
    16021 tggcgaagcg cgaagctgaa ggcagccaca tcatgctggg tgcgcaaaac gtggacctga
    16081 acctgtccgg cgcattcacc ggtgaaactt ctgccgctat gctgaaagac atcggcgctc
    16141 agtacatcat catcggccac tctgaacgtc gtacttacca caaagagtct gacgaactga
    16201 tcgcgaaaaa attcgcggtg ctgaaagagc agggcctgac tccggttctg tgcatcggtg
    16261 aaaccgaagc tgaaaacgaa gcgggcaaaa ctgaagaagt ttgcgcacgt cagatcgacg
    16321 cggtactgaa aactcagggt gctgcggcat tcgaaggtgc ggttatcgct tacgaacctg
    16381 tatgggcaat cggtactggc aaatctgcaa ctccggctca ggcacaggct gttcacaaat
    16441 tcatccgtga ccacatcgct aaagttgacg ctaacatcgc tgaacaagtg atcattcagt
    16501 acggcggctc tgtaaacgcg tctaacgctg cagaactgtt tgctcagcca gatatcgacg
    16561 gcgcgctggt tggcggtgct tctctgaaag ctgacgcttt cgcagtgatc gttaaagctg
    16621 cagaagcggc taaacaggct taagtctgac aggtgccgga tttcatatcc ggcacttact
    16681 ttccttaact cttcgcctta acgcaaaatc tcacactgat gatcctgaat ttcctcggct
    16741 gaagcacggt taagcgtcag tagatttcgt tgtgtcgcca gcaatacaaa tgagttatca
    16801 ctctgccgta ccatcgccag cccgtagctt cccatatgtt cccgcgcctc aggtacttct
    16861 tctgccagca tcataaatgg gctgcgttgt accagttcgc tttccgttac ccgacgcgcc
    16921 aggtattcat gcccacgcaa accacctggc agtggcaacc agcggctgct gatgttcgcc
    16981 agattgttat ccagttgttc gcgcacatca ggacgaatac aagagatatg aatatgaaaa
    17041 tggttttgcg tacgcccggt gcgggagttg atcgccaaag aaaccgcgcg atcgggaacc
    17101 ggctggccgt attttttgct cataaaatca cgcgcctgcc aggccaacca aaagaagttc
    17161 ggcgttgaag gatcggtcaa caaaggactt tcagtaccgt taatacgata cgttggcatc
    17221 aacagatatt gcagtggacc attaagatct tttaaaacca cgtatccggc attgggtttg
    17281 acttccgcac atggcgaagg attttgattt tgctgctgat tgggcaaaca ttcctcaagg
    17341 acaatcttac gtaatgtatc cgactcttca ccggttaatt tccagtaacc aataccggca
    17401 gccacaacgg cgataactat catcaccaaa aaaagaagac ccgctttttt catctttttt
    17461 tccctgtacc tcaaagagat gcaagggtaa cgcaaaatcg tgacaaataa aaaacccggt
    17521 cgcgagaaac caccgggttt gataattatc ctggggaaat cagcgtttgc tgatctgatc
    17581 gaacgtaccg ccgttagcaa aatgctcttt ttgcgctttc gtccagccgc cgaactcttc
    17641 atcaatggtg aataacttca gctttggaaa cgcattttcg tactttttcg ctacttcagc
    17701 gtcgcgcgga cggtagtagt ttttcgcggc aatttcctga ccttctggcg agtagagata
    17761 tttcaggtag gcttccgcca cctctttggt gccttttttc tcgaccactt tatcgaccac
    17821 cgacacggtt ggctctgcga ggatagactc actcggcgtg acgatttcga atttatcttt
    17881 ccccagttca ttcgctgcca gcagagcttc gttttcccag gcgatcagta catcgccaat
    17941 cccgcgctcg acaaaggtgt tagtggagcc gcgcgcgcca gaatccagaa cttcgacgtt
    18001 tttatacagt gcccgaacaa aatcctgtgc ttttgcctga tcgttgttgt tgtgatgcag
    18061 cgcgtagccc caggcagcta ggtagttcca gcgtgcgcca ccagagcttt tcggattagg
    18121 cgtgatcacc gaaacacccg gtttaatcag atcgttccag tcatggatct gcttcggatt
    18181 gcccttacgc accaggaaaa caatggtgga agtgtacggt gcggagttat ccggcagacg
    18241 tttgatccac tctttatcaa tccgcccacg ttccgcaatc gcgtccacgt cataggccag
    18301 agccagcgtg acaacatcag cttcaatacc gttgattacc gacgtcgctt gtttacctga
    18361 accaccgtgt gactgacgaa tcaccacgtt atcaccagtt tgctgtttcc agtgggcgct
    18421 gaatgccttg ttgtactgtt cgtacaattc gcgcgttgga tcatatgaaa cgttaagaag
    18481 ctgaatatcc tttgccataa cgctggttgc cgccagcaaa aatgttaacc ctacgcccca
    18541 cttgttcatc gcccgactct cttatgttgt gttgtgatga gcaaagcgtg ccagaaggtt
    18601 aaccaaacat taaagaataa aaaaagattg gctataactt gcgggtatat gttgagggat
    18661 taaaaaggcg gaagaaatcc gcctcatatt gctgacaaag tgcgctttgt ccatgccgga
    18721 tgcggcgcga acgccttatc cggcctacaa aagtttgcaa attcaataaa ttgcagaatt
    18781 catgtaggcc tgataagcga agcgcatcag gcatttttgc ttctgtcatc ggtttcaggc
    18841 taaaggaatc tgcctttttc cgaaatcatt aatacagttt tttcgcgcag tccagccagt
    18901 cacctttgaa cggacgcttc atgttttcga tagcgtcgat gatgtcgtgg tgaaccagct
    18961 gttcgttctg gatacctaca caacgaccgc cgtaacctgc cagcagcaga tcgatagcgt
    19021 aagcgcccat acgggaagcc agaatacggt cgtaaggcac cggagaacca ccgcgctgga
    19081 tgtggcccag cacagttgcg cgggtttcac gaccggtttc tttctcgatg aaatgcgcca
    19141 gttcgtcaac atcacacata tgttcggtaa tcgccacgat cgcgtgtttt ttacctttcg
    19201 cgatacccgc tttgatttcg tttaccaggt cttcacggct gaattcaact tccggaacca
    19261 caacgaattc acagccaccg gcaatggccg cagccaacgt cagatcgcca caataacggc
    19321 ccatcacttc caccacggaa atacgctggt gagaagaaga ggtgtcacgc agacggtcga
    19381 tcgcttctac aacggtgctc agcgcagtga agaaaccgat agtgtagtca gtgcctttga
    19441 tgtcgttgtc gatagtgccc ggcagaccga tgcacgggaa gcccatttcg gtcagacgca
    19501 ttgcacccat gtaggaaccg tcaccgccga taaccaccag cgcgtcgata ccacgttttt
    19561 tcaggttttc gatagccacg gcgcggatgt tctcgtcgcg gaattccggg aagcgcgcag
    19621 aaccgaggaa cgtaccgcca cggttgatca tgtcagaaac gctgtaacgg tctagctgta
    19681 ccatacggtc ttcatacaga cccagatagc cgtcataaat gcccattact tccagacctt
    19741 ctgtcagcgc agaacgaaca accccgcgaa ttgcggcgtt catgcctggc gcatcaccgc
    19801 cgcttgtcaa cacaccgatt ttcttaatca tgactacctc tgaactttgg aatgcaaaat
    19861 aaaatctgtt gccggaagtc ttcttgcaca tcgaagtgat ccaacgaatg tgcaaatagt
    19921 ataacaatca cttcctgctg aattgattca ggtcaggcca aatggcggta ttttatacac
    19981 aaaatgcggg tctggctctc ttttatactg attatgaaag catagaccgt ttaccctccc
    20041 tgggtacgac ggaacagggg tcctgatgga taattacatc cgatcccgga aaacgccgta
    20101 aaatagcctg ctctacctga tccgccacca tatgtgcctg aaccaaaggc agagagtctt
    20161 ccatttccaa atgaatctga ataaagcggg tcggccctga ctgccgcgtg cgaagatcgt
    20221 gagcgccgct aacacccggc caggaagtca cgatatcaat aatttcttgc cgttcctcat
    20281 caggcaatgc gcgatccagt aatgactgta ccgcctcata tcccatgcgt aacgcgctat
    20341 ataaaatata gatgccgatt cccaatgcaa acagagcatc ggcgcgatgc cagccgtacc
    20401 aggacaaccc cagcgccagc agaattgcgc cgttcatcat aacatcagac tggtaatgta
    20461 gcatatcagc ccgcaccgcc tggctttgcg tccggcgcac cacccaccgc tgaaacgaga
    20521 caaggataat cgtacaaatt agcgccacaa ttgtcacgat aaccccgacg cctggatctg
    20581 tcatcggtgt tggagatacc agatgttgaa tacccgtcaa aaacaggaat agtgccgaac
    20641 cggagataaa catactttgc gccagcgccg cgagggactc agctttaccg tgaccaaacg
    20701 agtgattatc gtcggcaggt tgcagggaat atcgcaccac cagtaaattc gtcaacgacg
    20761 cgccgatatc caccagcgaa tccaccagcg cggcgagaat actcaccgac ccggtatacc
    20821 accatgcaaa aattttaatc agcaatagca gcgaagccat cgccgtcgca gcaatcgccg
    20881 cccgactgac cagccgtcca taagattgat tcataaatac tcccactatc aactgacgct
    20941 agtataacgg aagcaaatca tctgcaatgc attaagcagc aggcaaattg aggataaaaa
    21001 aaaccccaca tcatgtgggg gaagacaggg atggtgtcta tggcaaggaa aacagggttt
    21061 actactggga acgtgagttg ctactactca atagcttcaa cgatgaactt ttttgccatt
    21121 gcgtcacgtc acgcaactgc tccattcgtt gttgatgttt ctcgtttaaa accgcttgct
    21181 gctccggcgt taacaggcga tacatttggt tgcggacttt tgccatctca acctgacgag
    21241 caatttgctc attcgccatt ttttctgcct gtgcgcgcac agcgttttca tcaaaatttt
    21301 ctgcggtgac aaggcgatgc attgtctcca gttcgctaac attaacagga ggctgttcgt
    21361 gccgggcctg ttgcataaga tctcgcatct gctgacgctg atgttcggtt aaacttatgc
    21421 cgtcgaacat atggctctgc gtactgcgct gcgtaagttc ttcacccgga tgccagttat
    21481 cgcctgaacc gacttcagca gcgtggctta atgaactgac tgccagcgtt gaggccatga
    21541 cggcagcggt aactatgcgc atcatttgct cccaaaatct ttctgtcgcg attcaacgat
    21601 agagagttta cgattcaggc tgcaaacatg cgtcaggggg tgtaaaacaa cgtaaagtca
    21661 tggattagcg acgtctgatg acgtaatttc tgcctcggag gtatttaaac aatgaataaa
    21721 atcctgttag ttgatgatga ccgagagctg acttccctat taaaggagct gctcgagatg
    21781 gaaggcttca acgtgattgt tgcccacgat ggggaacagg cgcttgatct tctggacgac
    21841 agcattgatt tacttttgct tgacgtaatg atgccgaaga aaaatggtat cgacacatta
    21901 aaagcacttc gccagacaca ccagacgcct gtcattatgt tgacggcgcg cggcagtgaa
    21961 cttgatcgcg ttctcggcct tgagctgggc gcagatgact atctcccgaa accgtttaat
    22021 gatcgtgagc tggtggcacg tattcgcgcg atcctgcgcc gttcgcactg gagcgagcaa
    22081 cagcaaaaca acgacaacgg ttcaccgaca ctggaagttg atgccttagt gctgaatcca
    22141 ggccgtcagg aagccagctt cgacgggcaa acgctggagt taaccggtac tgagtttacc
    22201 ctgctctatt tgctggcaca gcatctgggt caggtggttt cccgtgaaca tttaagccag
    22261 gaagtgttgg gcaaacgcct gacgcctttc gaccgcgcta ttgatatgca catttccaac
    22321 ctgcgtcgta aactgccgga tcgtaaagat ggtcacccgt ggtttaaaac cttgcgtggt
    22381 cgcggctatc tgatggtttc tgcttcatga taggcagctt aaccgcgcgc atcttcgcca
    22441 tcttctggct gacgctggcg ctggtgttga tgttggtttt gatgttaccc aagctcgatt
    22501 cacgccagat gaccgagctt ctggatagcg aacagcgtca ggggctgatg attgagcagc
    22561 atgtcgaagc ggagctggcg aacgatccgc ccaacgattt aatgtggtgg cggcgtctgt
    22621 tccgggcgat tgataagtgg gcaccgccag gacagcgttt gttattggtg accaccgaag
    22681 gccgcgtgat cggcgctgaa cgcagcgaaa tgcagatcat tcgtaacttt attggtcagg
    22741 ccgataacgc cgatcatccg cagaagaaaa agtatggccg cgtggaactg gtcggtccgt
    22801 tctccgtgcg tgatggcgaa gataattacc aactttatct gattcgtccg gccagcagtt
    22861 ctcaatccga tttcattaac ttactgtttg accgcccgct attactgctg attgtcacca
    22921 tgttggtcag tacgccgctg ctgttgtggt tggcctggag tctggcaaaa ccggcgcgta
    22981 agctgaaaaa cgctgccgat gaagttgccc agggaaactt acgccagcac ccggaactgg
    23041 aagcggggcc acaggaattc cttgccgcag gtgccagttt taaccagatg gtcaccgcgc
    23101 tggagcgcat gatgacctct cagcagcgtc tgctttctga tatctctcac gagctgcgca
    23161 ccccgctgac gcgtctgcaa ctgggtacgg cgttactgcg ccgtcgtagc ggtgaaagca
    23221 aggaactcga gcgtattgaa accgaagcgc aacgtctgga cagcatgatc aacgatctgt
    23281 tggtgatgtc acgtaatcag caaaaaaacg cgctggttag cgaaaccatc aaagccaacc
    23341 agttgtggag tgaagtgctg gataacgcgg cgttcgaagc cgagcaaatg ggcaagtcgt
    23401 tgacagttaa cttcccgcct gggccgtggc cgctgtacgg caatccgaac gccctggaaa
    23461 gtgcgctgga aaacattgtt cgtaatgctc tgcgttattc ccatacgaag attgaagtgg
    23521 gctttgcggt agataaagac ggtatcacca ttacggtgga cgacgatggt cctggcgtta
    23581 gcccggaaga tcgcgaacag attttccgtc cgttctatcg gaccgatgaa gcacgcgatc
    23641 gtgaatctgg cggtacagga ttggggctgg cgattgttga aaccgccatt cagcagcatc
    23701 gtggctgggt gaaggcagaa gacagcccgc tgggcggttt acggctggtg atttggttgc
    23761 cgctgtataa gcggagttaa actccgcatt tgtaggcagg ataaggcgtt tacgccgcat
    23821 ccggcatttg agcaggatgc ctgatgcgac gctgatagcg tcttatcagg cctacactcc
    23881 gggggttatt ttccccacaa ccggcgggaa aaatcttcga tcttgccgct cagtcggcgc
    23941 ttttgcatcg tccgcgtcca gcttttcgat aacccggcgg cggagagtaa acggtgatac
    24001 tgatcgtcat caaacggcat atgccatgcg atggcggcag cctcctgcac ggtcacatcg
    24061 ctgacacggg aaaccaactc cagcggcgcg tccgcagaca ctttccctgg cgctatcacg
    24121 ctatacaacc accccacctt acctgtgttt tgcatcaact gcgctatatc gctgatatca
    24181 aaatggtaat tgagtttata gcaaggcgag cgcggctggc tgacctgaat taatgcctct
    24241 ccccagcgga aaatatcgcc catatgaaca ttactttccg tgagaccgtc ggttgagaga
    24301 ttttcgccaa acgcaggcgc aacaaacaac tccgcctgtt ccggaaattc ccgcacccag
    24361 tagagatagt gctcacgagg ataatgacac agcgctctgt ctggcccgcc gtgaaccttt
    24421 ttctccgcct gctcgtcacc ttccagcccc agctccgtca gcatcaactc accatcaacc
    24481 tggattttgg caattgcgct gggtttgcct tcgggataag cctgaatctt gcctgtgtat
    24541 acatcaaccg gatatcgcat ctgttccctc tccacgagaa atttccccat tagaccatat
    24601 ttattttatg tcaggcgtgc tgttttcgcg gtaagtatcc aggcaggaac tcaatagcaa
    24661 accggacgaa cgttagttca tccggctgtg gggattaagg aagcaggttt atttcaccgt
    24721 acctaaaatt tcgattttcg ctgctctggc tttgacttta cgtgaccaga tagaggtaag
    24781 aattggcacc agaatcgaag tcacaatgac cgccgtcgct accagcgaag ttgctgccgg
    24841 agccatcggt ttaaacgcag gcaccatttc tgcaatcagc acaggtgtcg ctaccgcggc
    24901 ccctgcggaa ctggaagcgg caattccggc agtgccatcg ccaccgccaa tcaatttatc
    24961 ggcgataatc aacggaatac cggtcacgat aattactgcc acacccaaca ggatccccag
    25021 caaaccagtc tgggcaatca cagtcaaatc aatggtattg cccagcgcga aggcaaagaa
    25081 tggaatcagc gtttgcactg ctttgctgaa aaattctcgt aattcagggt caagattccc
    25141 cagggcaaag cccaccagga acggtaatac tgcgccgacg aaaacatgcg gttcaaacga
    25201 ggcaatcccg gcagtgccca gaataatcat cgtcatgagc ggcccggact ccaacgacat
    25261 caacacaaat gccccagctt cttctttcgt accgtactgc tgcatgatgg aagcgtaaag
    25321 tccgccgttg gtcatatcca tcgccgccac cagcgccagc gttgaaagtc cggcaaagaa
    25381 tccaacttca acaccatgtt ccggaataat gcgcgaggca attgccgcaa ccacccacgc
    25441 gacggcaatt ttagttacca ccagcgtacc ggatttacgc agtaccgttc ccgtcgcgct
    25501 taattttatt gacgccccca tgcaaaaaaa ccacaccgcc agaatgggca ccgtaccggt
    25561 aatcatcccg ttggtgaatg atccaaaata tttccccgcc cccggcgaga aggtgtggca
    25621 cagtgcgcca aggaatagcg ggacgagcat catccccccc gggattttct caatcgagcg
    25681 ttttatctgc atttccatca cctgtcggca tatttttaag ggctgatgcg taattcctca
    25741 atggagcatc agtgttgatg gaaacgaaga taga
//
LOCUS       DAZPAC010000043        25494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZPAC010000043 DAZPAC010000000
VERSION     DAZPAC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.043"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..485
                     /gene="gspA"
                     /locus_tag="ACHUPM_001446"
     CDS             <1..485
                     /gene="gspA"
                     /locus_tag="ACHUPM_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR0869271.1"
                     /translation="SLQTLVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTW
                     TLTHKWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAE
                     WRPLLVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK
                     "
     gene            487..906
                     /gene="gspB"
                     /locus_tag="ACHUPM_001447"
     CDS             487..906
                     /gene="gspB"
                     /locus_tag="ACHUPM_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFR0869272.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1144..1455
                     /gene="rpsJ"
                     /locus_tag="ACHUPM_001448"
     CDS             1144..1455
                     /gene="rpsJ"
                     /locus_tag="ACHUPM_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR0869273.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1488..2117
                     /gene="rplC"
                     /locus_tag="ACHUPM_001449"
     CDS             1488..2117
                     /gene="rplC"
                     /locus_tag="ACHUPM_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR0869274.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2128..2733
                     /gene="rplD"
                     /locus_tag="ACHUPM_001450"
     CDS             2128..2733
                     /gene="rplD"
                     /locus_tag="ACHUPM_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR0869275.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2730..3032
                     /gene="rplW"
                     /locus_tag="ACHUPM_001451"
     CDS             2730..3032
                     /gene="rplW"
                     /locus_tag="ACHUPM_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR0869276.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3050..3871
                     /gene="rplB"
                     /locus_tag="ACHUPM_001452"
     CDS             3050..3871
                     /gene="rplB"
                     /locus_tag="ACHUPM_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR0869277.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3888..4166
                     /gene="rpsS"
                     /locus_tag="ACHUPM_001453"
     CDS             3888..4166
                     /gene="rpsS"
                     /locus_tag="ACHUPM_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR0869278.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4181..4513
                     /gene="rplV"
                     /locus_tag="ACHUPM_001454"
     CDS             4181..4513
                     /gene="rplV"
                     /locus_tag="ACHUPM_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR0869279.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4531..5232
                     /gene="rpsC"
                     /locus_tag="ACHUPM_001455"
     CDS             4531..5232
                     /gene="rpsC"
                     /locus_tag="ACHUPM_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR0869280.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5245..5655
                     /gene="rplP"
                     /locus_tag="ACHUPM_001456"
     CDS             5245..5655
                     /gene="rplP"
                     /locus_tag="ACHUPM_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR0869281.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5655..5846
                     /gene="rpmC"
                     /locus_tag="ACHUPM_001457"
     CDS             5655..5846
                     /gene="rpmC"
                     /locus_tag="ACHUPM_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR0869282.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5846..6100
                     /gene="rpsQ"
                     /locus_tag="ACHUPM_001458"
     CDS             5846..6100
                     /gene="rpsQ"
                     /locus_tag="ACHUPM_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR0869283.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6265..6636
                     /gene="rplN"
                     /locus_tag="ACHUPM_001459"
     CDS             6265..6636
                     /gene="rplN"
                     /locus_tag="ACHUPM_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR0869284.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6647..6961
                     /gene="rplX"
                     /locus_tag="ACHUPM_001460"
     CDS             6647..6961
                     /gene="rplX"
                     /locus_tag="ACHUPM_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR0869285.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6976..7515
                     /gene="rplE"
                     /locus_tag="ACHUPM_001461"
     CDS             6976..7515
                     /gene="rplE"
                     /locus_tag="ACHUPM_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR0869286.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7530..7835
                     /gene="rpsN"
                     /locus_tag="ACHUPM_001462"
     CDS             7530..7835
                     /gene="rpsN"
                     /locus_tag="ACHUPM_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR0869287.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7869..8261
                     /gene="rpsH"
                     /locus_tag="ACHUPM_001463"
     CDS             7869..8261
                     /gene="rpsH"
                     /locus_tag="ACHUPM_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR0869288.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8274..8807
                     /gene="rplF"
                     /locus_tag="ACHUPM_001464"
     CDS             8274..8807
                     /gene="rplF"
                     /locus_tag="ACHUPM_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR0869289.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8817..9170
                     /gene="rplR"
                     /locus_tag="ACHUPM_001465"
     CDS             8817..9170
                     /gene="rplR"
                     /locus_tag="ACHUPM_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR0869290.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9185..9688
                     /gene="rpsE"
                     /locus_tag="ACHUPM_001466"
     CDS             9185..9688
                     /gene="rpsE"
                     /locus_tag="ACHUPM_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR0869291.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9692..9871
                     /gene="rpmD"
                     /locus_tag="ACHUPM_001467"
     CDS             9692..9871
                     /gene="rpmD"
                     /locus_tag="ACHUPM_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR0869292.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9875..10309
                     /gene="rplO"
                     /locus_tag="ACHUPM_001468"
     CDS             9875..10309
                     /gene="rplO"
                     /locus_tag="ACHUPM_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR0869293.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10317..11648
                     /gene="secY"
                     /locus_tag="ACHUPM_001469"
     CDS             10317..11648
                     /gene="secY"
                     /locus_tag="ACHUPM_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR0869294.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11680..11796
                     /gene="rpmJ"
                     /locus_tag="ACHUPM_001470"
     CDS             11680..11796
                     /gene="rpmJ"
                     /locus_tag="ACHUPM_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR0869295.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11943..12299
                     /gene="rpsM"
                     /locus_tag="ACHUPM_001471"
     CDS             11943..12299
                     /gene="rpsM"
                     /locus_tag="ACHUPM_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR0869296.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12316..12705
                     /gene="rpsK"
                     /locus_tag="ACHUPM_001472"
     CDS             12316..12705
                     /gene="rpsK"
                     /locus_tag="ACHUPM_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR0869297.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12739..13359
                     /gene="rpsD"
                     /locus_tag="ACHUPM_001473"
     CDS             12739..13359
                     /gene="rpsD"
                     /locus_tag="ACHUPM_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR0869298.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13385..14374
                     /gene="rpoA"
                     /locus_tag="ACHUPM_001474"
     CDS             13385..14374
                     /gene="rpoA"
                     /locus_tag="ACHUPM_001474"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR0869299.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14415..14798
                     /gene="rplQ"
                     /locus_tag="ACHUPM_001475"
     CDS             14415..14798
                     /gene="rplQ"
                     /locus_tag="ACHUPM_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR0869300.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14905..15273
                     /locus_tag="ACHUPM_001476"
     CDS             14905..15273
                     /locus_tag="ACHUPM_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFR0869301.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15284..15709
                     /gene="zntR"
                     /locus_tag="ACHUPM_001477"
     CDS             15284..15709
                     /gene="zntR"
                     /locus_tag="ACHUPM_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFR0869302.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15765..15983
                     /gene="arfA"
                     /locus_tag="ACHUPM_001478"
     CDS             15765..15983
                     /gene="arfA"
                     /locus_tag="ACHUPM_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFR0869303.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="ACHUPM_001479"
     CDS             complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="ACHUPM_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR0869304.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="ACHUPM_001480"
     CDS             complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="ACHUPM_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR0869305.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="ACHUPM_001481"
     CDS             complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="ACHUPM_001481"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR0869306.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="ACHUPM_001482"
     CDS             complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="ACHUPM_001482"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR0869307.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20215..20724)
                     /gene="def"
                     /locus_tag="ACHUPM_001483"
     CDS             complement(20215..20724)
                     /gene="def"
                     /locus_tag="ACHUPM_001483"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR0869308.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20854..21978
                     /gene="dprA"
                     /locus_tag="ACHUPM_001484"
     CDS             20854..21978
                     /gene="dprA"
                     /locus_tag="ACHUPM_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR0869309.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21950..22423
                     /gene="smg"
                     /locus_tag="ACHUPM_001485"
     CDS             21950..22423
                     /gene="smg"
                     /locus_tag="ACHUPM_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR0869310.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22452..22994
                     /locus_tag="ACHUPM_001486"
     CDS             22452..22994
                     /locus_tag="ACHUPM_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR0869311.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22999..23571
                     /gene="tsaC"
                     /locus_tag="ACHUPM_001487"
     CDS             22999..23571
                     /gene="tsaC"
                     /locus_tag="ACHUPM_001487"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR0869312.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23576..24394
                     /gene="aroE"
                     /locus_tag="ACHUPM_001488"
     CDS             23576..24394
                     /gene="aroE"
                     /locus_tag="ACHUPM_001488"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR0869313.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24391..24648
                     /locus_tag="ACHUPM_001489"
     CDS             24391..24648
                     /locus_tag="ACHUPM_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR0869314.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24624..25178)
                     /locus_tag="ACHUPM_001490"
     CDS             complement(24624..25178)
                     /locus_tag="ACHUPM_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR0869315.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6492 a         6023 c         6679 g         6300 t
ORIGIN      
        1 cttctttaca aacattggta gatcagaatt tgccctggag cgcatcgctt aaagtcgggg
       61 acaaaaaatt gcctgttgtg gtcgttcgcg tcggtgaggc tagcgttgat gtcctcgtag
      121 gtcagcaaac gtggacgctg acacataaat ggtttgagtc ggtatggacg ggtgattatc
      181 ttctgttgtg gaaaatgtcg ccggaagggg agagtaccat tacgcgtgat agcagcgagg
      241 aagagattct ctggctggaa acgatgttaa accgtgcatt gcatatttcg acagaacctt
      301 cggcggagtg gcgtcccttg ttggtagaaa aaataaagca atttcaaaaa agccaccact
      361 tgaaaactga cggtgttgtt ggcttttcaa cgttagtaca tctctggcag gtggcagggg
      421 agagtgccta cttatatcgg gatgaagcaa atatttcccc ggaaacaaca gtgaagggga
      481 aatgacatgt ttgaattcta tatcgccgcg cgtgagcaga aagaaacagg tcaccctggt
      541 atcttctccc gacaaaaaca cagcaccata atatatgtca tttgtttatt gctgatttgc
      601 ctgtggtttg caggaatggt gttggttggt gggtatgcca ggcagctatg ggcgctttgg
      661 atagtaaaag cagaagtcac tgtagaggct gaaacgcccg cttttaaaca atcaacacaa
      721 cactatttct tcaaaaaaca gccactcccc gtcgtcgaat ccgtcgagga ggaagatgac
      781 ccagacgtcg cggttgagaa tgctccctct tcttctgaag acgaagaaaa tactgtaaaa
      841 gaaagcgacg agaaagccgg tttaagagag cgtgtcaaaa atgcgctgaa cgaacttgag
      901 agataacccg aaggctgttt actttactag caatacgctt gcgttcggtg gttaagtatg
      961 tataatgcgc gggcttgtcg tagttgacag caggttcaat ctgaacctca gttagccgaa
     1021 tttggctacc taacaatgct cccaatcggg gagctacgta agaacggtta cactctccca
     1081 tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat aaaataattg gagctctggt
     1141 ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat
     1201 caagcaaccg cggaaatcgt cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg
     1261 atcccgctgc cgacacgcaa agagcgcttc actgttctga tctccccgca cgtcaacaaa
     1321 gacgcgcgcg atcagtacga aatccgtact cacttgcgtc tggttgacat cgttgagcca
     1381 accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag
     1441 atcagcctgg gttaatcagg tcattgagcg attgagaggt tgaaacaatg attggtttag
     1501 tcggtaaaaa agtgggtatg acccgtatct tcacagaaga cggcgtttct atcccagtaa
     1561 ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa agacctggct aacgatggct
     1621 accgtgctat tcaggtgacc accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag
     1681 ctggccactt cgctaaagct ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg
     1741 ctgaaggcga agagttcact gtaggtcaga gcattagcgt tgaactgttt gctgaggtta
     1801 aaaaagttga cgtaactggc acctctaaag gtaaaggttt cgcaggtacc gttaagcgct
     1861 ggaacttccg tacccaggac gctactcacg gtaactcctt gtctcaccgc gttccgggtt
     1921 ctatcggtca gaaccagact ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga
     1981 tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt acgcgttgac gctgagcgca
     2041 acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac
     2101 cagctgtgaa ggcgtaagga gatagcaatg gaattagtat tgaaagacgc gcagagcgcg
     2161 ctgactgttt ccgaaactac cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt
     2221 gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa
     2281 gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca ccggccgtgc gcgttctggt
     2341 tctatcaaga gcccgatctg gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac
     2401 cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa
     2461 ctggtacgtc aggatcgtct gatcgttgtc gagaagttct ctgtagaagc gccgaaaact
     2521 aagctgctgg cacagaaact gaaagacatg gctctggaag atgtgctgat catcaccggt
     2581 gagctggacg aaaacctgtt cctggctgcg cgcaacctgc acaaggttga cgtacgcgat
     2641 gcaactggta tcgacccggt tagcctgatc gccttcgaca aagtcgtaat gactgctgat
     2701 gctgttaagc aagttgagga gatgctggca tgattcgtga agaacgtctg ctgaaggtgc
     2761 tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg
     2821 tactcaaagt tgctaaagac gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt
     2881 ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa agggaaagtt aaacgtcacg
     2941 gacagcgtat cggtcgtcgt agcgactgga aaaaagctta cgtcaccctg aaagaaggcc
     3001 agaatctgga cttcgttggc ggcgctgagt aagtcggagg agtaatacaa tggcagttgt
     3061 taaatgtaaa ccgacatctc cgggtcgtcg ccacgtagtt aaagtggtta accctgagct
     3121 gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa
     3181 caacaatggc cgtatcacca ctcgtcatat cggtggtggc cacaagcagg cttaccgtat
     3241 tgttgacttc aaacgcaaca aagacggtat cccggcagtt gttgaacgtc ttgagtacga
     3301 tccgaaccgt tccgcgaaca tcgcgctggt tctgtacaaa gacggtgaac gccgttacat
     3361 cctggcccct aaaggcctga aagctggcga ccagattcag tctggcgttg atgctgcaat
     3421 caaaccaggt aacaccctgc cgatgcgcaa catcccggtt ggttctactg ttcataacgt
     3481 agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc gctggtactt acgttcagat
     3541 cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt
     3601 agaagcagac tgccgtgcaa ctctgggcga agttggcaat gctgagcata tgctgcgcgt
     3661 tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc
     3721 gatgaacccg gtagaccacc cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc
     3781 ggtaactccg tggggcgttc agaccaaagg taagaagacc cgcagcaaca agcgtactga
     3841 taaattcatc gtacgtcgcc gtagcaaata attttagagg ataagccatg ccacgttctc
     3901 tcaagaaagg tccttttatt gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa
     3961 gcggagacaa gaagcccctg cgcacttggt cccgtcgttc aacgatcttt cctaacatga
     4021 tcggtttgac catcgctgtc cataatggtc gtcagcacgt tccggtattt gtgaccgacg
     4081 aaatggtcgg tcacaaactg ggtgaattcg caccgactcg tacttatcgc ggccacgctg
     4141 ctgataaaaa agcgaagaag aaataaggta ggaggaagag atggaaacta tcgctaaaca
     4201 tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa
     4261 gaaagtgtcg caggctctgg atattttgac ctacaccaac aagaaagcgg ctgtactggt
     4321 caagaaagtt ctggaatctg ccattgctaa cgctgaacac aacgatggcg ctgacattga
     4381 cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc
     4441 gcgtgcaaaa ggtcgtgcag atcgcatcct gaagcgcacc agccacatca ctgtggttgt
     4501 gtccgatcgc tgagactctg gagactagca atgggtcaga aagtacatcc taatggtatt
     4561 cgcttgggta ttgtaaaacc atggaactct acctggtttg cgaacaccaa agaattcgct
     4621 gacaacctgg acagcgattt taaagtacgt cagtacctga ctaaggaact ggctaaagcg
     4681 tctgtatctc gtatcgttat cgagcgtccg gctaagagca tccgtgtaac cattcacact
     4741 gctcgcccgg gtatcgttat cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc
     4801 gtagcggaca tcgctggcgt tcctgcacag atcaacatcg ccgaagttcg taagcctgaa
     4861 ctggacgcaa aactggttgc tgacagcatc acttctcagc tggaacgtcg cgttatgttc
     4921 cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa
     4981 gttgaagtta gcggccgtct gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa
     5041 ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact acaacacctc tgaagcgcac
     5101 accacttacg gtgtaatcgg cgttaaagtg tggatcttca aaggcgagat cctgggtggt
     5161 atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa
     5221 ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg
     5281 cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg ttagcttcgg cagcttcggt
     5341 ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct
     5401 atgacccgtg cagttaagcg tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg
     5461 atcactgaaa agccgctggc agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg
     5521 gttgccttga ttcagccggg taaagtcctg tatgaaatgg acggtgttcc ggaagagctg
     5581 gcccgtgaag cattcaagct ggcagcagcg aaactgccga ttaaaaccac ctttgtaact
     5641 aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca
     5701 ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc
     5761 agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt
     5821 tactgaacga gaaggcgggt gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt
     5881 tgttagcgac aaaatggaga aatccattgt tgttgctatc gaacgttttg tgaaacaccc
     5941 gatctacggt aaattcatca agcgtacgac caaactgcac gtacatgacg agaacaacga
     6001 atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc
     6061 ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa tacagtacac tctctcgata
     6121 cgaataaacg gctcagaaat gagccgttta ttttttctac ccatatcctt gaagcggtgt
     6181 tataatgccg cgccctcgat atggggattt ttaacgacct gattttcggg tctcagtagt
     6241 agttgacatt agcggagcac taaaatgatc caagaacaga ctatgctgaa cgtcgccgac
     6301 aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac
     6361 gcaggcgtag gcgacatcat caagatcacc atcaaagaag caattccgcg tggtaaggtc
     6421 aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg
     6481 gacggttctg tcattcgctt cgatggtaat gcttgtgttc ttctgaacaa caacagcgag
     6541 cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg agcttcgtag tgagaagttc
     6601 atgaaaatta tctctctggc accagaagta ctctaaggag cgaatcatgg cagcgaaaat
     6661 ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt
     6721 taagaatgtc ctgtcttccg gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca
     6781 tcagaagccg gttccggccc tgaaccaacc gggtggcatc gttgaaaaag aagccgctat
     6841 tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt
     6901 tagattcgaa gacggcaaaa aagtccgttt cttcaagtct aacagcgaaa ctatcaagta
     6961 atttggagta gtacgatggc gaaactgcat gattactaca aagacgaagt agttaaaaaa
     7021 ctcatgactg agtttaacta caattctgtc atgcaagtcc ctcgggtcga gaagatcacc
     7081 ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac tgctggataa cgcagcagca
     7141 gacctggcag caatctccgg tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca
     7201 ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag taactctgcg tggcgaacgc
     7261 atgtgggagt tctttgagcg cctgatcact attgctgtac ctcgtattcg tgacttccgt
     7321 ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag
     7381 atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc
     7441 attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc
     7501 ccgttccgca agtaaggtag ggttactaaa tggctaagca atcaatgaaa gcacgcgaag
     7561 taaaacgcgt agctttagct gataaatact tcgcgaaacg cgctgaactg aaagcgatca
     7621 tctctgatgt gaacgcttcc gacgaagatc gttggaacgc tgttctcaag ctgcagactc
     7681 tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgcccgc
     7741 atggtttcct gcggaagttc gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg
     7801 gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc accaattgaa tcacgggagt
     7861 aaagacagat gagcatgcaa gatccgatcg cggatatgct gacccgtatc cgtaacggtc
     7921 aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca
     7981 acgtgctgaa ggaagaaggt tttattgaag attttaaagt tgaaggcgac accaagcctg
     8041 aactggaact tactctgaag tatttccagg gcaaagctgt tgtagaaagc attcagcgtg
     8101 tcagccgccc aggtctgcgc atctataaac gtaaagatga gctgccgaaa gttatggcgg
     8161 gtctgggtat cgcagttgtt tctacctcta aaggtgttat gactgatcgt gcagcgcgcc
     8221 aggctggtct tggtggcgaa attatctgct acgtagccta atcggaggaa aaaatgtctc
     8281 gtgttgctaa agcaccggtc gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg
     8341 ttattacgat caaaggtaaa aacggcgagc tgactcgtac tctcaacgat gctgttgaag
     8401 ttaaacatgc agataatacc ctgaccttcg gtccgcgtga tggttacgca gacggttggg
     8461 cacaggctgg taccgcgcgt gccctgctga actcaatggt tatcggtgtt accgaaggct
     8521 tcactaagaa gctgcagctg gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga
     8581 ttaacctgtc tctgggtttc tctcatcctg ttgaccatca gctgcctgcg ggtatcactg
     8641 ctgaatgtcc gactcagact gaaatcgtgc tgaaaggcgt tgataagcag gtgatcggcc
     8701 aggttgcagc ggatctgcgc gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc
     8761 gttacgccga cgaagtcgtg cgtaccaaag aggctaagaa gaagtaaggt aacactatgg
     8821 ataagaaatt tgctcgtatc cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg
     8881 gcgcaactcg cctggtggta catcgtaccc cgcgtcacat ttacgcacag gtaattgcac
     8941 cgaacggttc tgaagttctg gtagctgctt ctactgtaga aaaagctatc gctgaacaac
     9001 tgaagtacac cggtaacaaa gacgcggctg cagctgtggg taaagctgtc gctgaacgcg
     9061 ctctggaaaa aggcatcaaa gatgtatcct ttgaccgttc cgggttccaa tatcatggtc
     9121 gtgtccaggc actggcagat gctgcccgtg aagctggcct tcagttctaa ggtagaggtg
     9181 taagatggct cacatcgaaa aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa
     9241 ccgcgtatct aaaaccgtta aaggtggtcg tattttctcc ttcacagctc tgactgtagt
     9301 tggcgatggt aacggtcgcg ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc
     9361 gatccagaaa gcgatggaaa aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg
     9421 cactctgcaa caccctgtta aaggtgttca cacgggttct cgcgtattca tgcagccggc
     9481 ttccgaaggt accggtatca tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg
     9541 ggttcataac gttctggcta aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc
     9601 aactattgat ggcctggaaa atatgaattc tccagaaatg gtcgctgcca agcgtggtaa
     9661 atccgttgaa gaaattctgg ggaaataaac catggcaaag actattaaaa ttactcaaac
     9721 ccgcagtgca atcggtcgtc tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg
     9781 tcgtattggt cacaccgtag agcgcgagga tactcctgct attcgcggta tgatcaacgc
     9841 ggtttccttc atggttaaag ttgaggagta agagatgcgt ttaaatactc tgtctccggc
     9901 cgaaggctcc aaaaaggcgg gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa
     9961 aaccggtggt cgtggtcaca aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg
    10021 tttcgagggt ggtcagatgc ctctgtaccg tcgtctgccg aaattcggct tcacttctcg
    10081 taaagcagcg attacagccg aagttcgtct gtctgacctg gctaaagtag aaggcggtgt
    10141 tgtagacctg aacacgctga aagcggctaa cattatcggt atccagatcg agttcgcgaa
    10201 agtgatcctg gctggcgaag taacgactcc ggtaactgtt cgtggcctgc gtgttactaa
    10261 aggcgctcgt gctgctatcg aagctgctgg cggtaaaatc gaggaataag tagcagatgg
    10321 ctaaacaacc gggattagat tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca
    10381 gactgctgtt tgttatcggt gcgctgattg tgttccgtat tggctctttt attccgatcc
    10441 ctggtattga tgccgctgta cttgccaaac tgcttgagca acagcgaggc accatcattg
    10501 agatgtttaa catgttctct ggtggtgctc tcagccgtgc ttctatcttt gctctgggga
    10561 tcatgccgta tatttcggcg tcgatcatta tccagctgct gacggtggtt cacccaacgt
    10621 tggcggaaat taagaaagaa ggggagtctg gtcgtcgtaa gatcagccag tacacccgct
    10681 acggtactct ggtgctggca atattccagt cgatcggtat tgctactggt ctgccgaata
    10741 tgcctggtat gcaaggcctg gtgattaacc cgggctttgc attctacttc accgctgttg
    10801 taagtctggt cacaggaacg atgttcctga tgtggttggg cgaacagatt actgaacgag
    10861 gtatcggcaa cggtatttca atcattatct tcgtcggtat tgtcgcggga ctcccgccag
    10921 ccattgccca tactatcgag caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt
    10981 tggttgcagt attagtattt gcagtgacgt tctttgttgt atttgttgag cgtggtcaac
    11041 gccgcattgt ggtaaactac gcgaaacgtc agcaaggtcg tcgtgtttat gctgcacaga
    11101 gcacacattt accgctgaaa gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca
    11161 gtattattct gttcccggcg accatcgcgt catggttcgg gggcggtact ggttggaact
    11221 ggctgacaac aatttcgctg tatttgcagc ctgggcaacc gctttatgtg ttactctatg
    11281 cgtctgcaat catcttcttc tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa
    11341 cagcagataa cctgaagaag tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa
    11401 cggcgaagta tatcgataaa gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta
    11461 cctttatctg cctgatcccg gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg
    11521 gtgggacctc actgcttatc gttgttgtcg tgattatgga ctttatggct caagtgcaaa
    11581 ctctgatgat gtccagtcag tatgagtctg cattgaagaa ggcgaacctg aaaggctacg
    11641 gccgataatt ggtcgcccga gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc
    11701 aagaaattat gccgtaactg caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc
    11761 agtgccgagc cgaagcataa acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa
    11821 agttgggttg agctggctag attagtcagc caatcttttg tatgtctgtg cgtttccatt
    11881 tgagtatcct gaaaacgggc ttttcagcat ggaacgtaca tattaaatag taggagtgca
    11941 tagtggcccg tatagcaggc attaacattc ctgatcataa gcatgccgta atcgcattaa
    12001 cttcgattta tggcgtcggc aagacccgtt ctaaagccat cctggctgca gcgggtatcg
    12061 ctgaagatgt taagatcagt gagctgtctg aaggacaaat cgacacgctg cgtgacgaag
    12121 ttgccaaatt tgtcgttgaa ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc
    12181 tgatggatct tggttgctat cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc
    12241 agcgtaccaa gaccaacgca cgtacccgta agggtccgcg caaaccgatc aagaaataat
    12301 cggggtgatt gaataatggc aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa
    12361 gtctctgacg gcgtggctca tatccatgct tctttcaaca acaccatcgt gactatcact
    12421 gatcgtcagg gtaacgcgtt gggttgggca acagccggtg gttccggttt ccgtggttct
    12481 cgcaaatcca ctccgtttgc agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa
    12541 gaatacggca tcaagaatct ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct
    12601 actattcgtg ctctgaacgc cgcaggtttc cgcatcacta acattactga tgtgactccg
    12661 atccctcata acggttgtcg tccgccgaaa aaacgtcgcg tataacgcct cgttttccag
    12721 gtttgttgga gaaagaaaat ggcaagatat ttgggtccta agctcaagct gagccgtcgt
    12781 gagggcaccg acttattcct taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt
    12841 gaacaagctc ctggccagca cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag
    12901 ttgcgtgaaa agcaaaaagt tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac
    12961 tactacaaag aagcagcacg tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg
    13021 gaaggtcgtc tggacaacgt tgtataccgt atgggcttcg gtgccactcg tgcagaagca
    13081 cgtcagctgg ttagccataa agcaattatg gtaaacggtc gtgttgttaa catcgcttct
    13141 tatcaggtta gtccgaatga cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc
    13201 gtgaaagccg ctctggagct ggctgagcag cgtgaaaagc caacctggct ggaagttgat
    13261 gctggcaaga tggaaggtac gtttaagcgt aagccggagc gttctgatct gtctgcggac
    13321 attaacgaac acctgatcgt cgagctttac tccaagtaaa gcttagtacc aaagagagga
    13381 cacaatgcag ggttctgtga cagagtttct aaaaccgcgc ctggttgata tcgagcaagt
    13441 gagttcgacg cacgccaagg tgacccttga gcctttagag cgtggctttg gccatactct
    13501 gggtaacgca ctgcgccgta ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt
    13561 tgagattgat ggtgtactac atgagtacag caccaaagaa ggcgttcagg aagatatcct
    13621 ggaaatcctg ctcaacctga aagggctggc ggtgagagtt cagggcaaag atgaagttat
    13681 tcttaccttg aataaatctg gcattggccc tgtgactgca gccgatatca cccacgacgg
    13741 tgatgtcgaa atcgtcaagc cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc
    13801 tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat
    13861 tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc
    13921 tgtggagcgt attgcctaca atgttgaagc agcgcgtgta gaacagcgta ccgacctgga
    13981 caagctggtc atcgaaatgg aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg
    14041 tgcggcaacc attctggctg aacaactgga agctttcgtt gacttacgtg atgtacgtca
    14101 gcctgaagtg aaagaagaga aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga
    14161 tctggaattg actgtccgct ctgctaactg ccttaaagca gaagctatcc actatatcgg
    14221 tgatctggta cagcgtaccg aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc
    14281 tcttactgag attaaagacg tgctggcttc ccgtggactg tctctgggca tgcgcctgga
    14341 aaactggcca ccggcaagca tcgctgacga gtaaccggat cacaggttaa ggttttactg
    14401 agaaggataa ggtcatgcgc catcgtaaga gtggtcgtca actgaaccgc aacagcagcc
    14461 atcgccaggc tatgttccgc aatatggcag gttcactggt tcgtcatgaa atcatcaaga
    14521 cgactctgcc taaagcgaaa gagctgcgcc gcgtagttga gccgctgatt actcttgcca
    14581 agactgatag cgttgctaat cgtcgtctgg catttgcccg tactcgtgat aacgagatcg
    14641 tggcaaaact gtttaacgaa ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc
    14701 gtattctgaa gtgtggcttc cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg
    14761 ttgatcgttc agagaaagca gaagctgctg cagagtaatc tgaagcaatg taaaaaaacc
    14821 cgccccggcg ggttttttta tacccgtagt atccccactt atctacaata gctgtactct
    14881 ttttgttcat cccctggagt atttatgtgg ttacttgacc agtgggcaga gcgccatata
    14941 gcagaagcgc aagcgaaagg tgagtttgat aacctggcag gtagcggcga accattgata
    15001 ctggatgatg attctcacgt gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat
    15061 gccggttgct taccgccaga acttgagcaa cggagagaag caattcagct tctggatatt
    15121 ctcaaaggta tccgtcacga tgatccgcaa tatcaagagg ttagccgtcg attgtcatta
    15181 ctggaattga agctgcgaca agctggattg agtaccgatt ttttacgcgg cgattatgct
    15241 gacaagttgt tggacaaaat caacgataac tagtggagta tgtatgtatc gcattggtga
    15301 gctggcaaaa atggcggaag taacacctga cacgattcgt tattacgaaa aacagcagat
    15361 gatggagcat gaagtgcgta ctgaaggcgg gtttcgcctg tataccgaaa gcgatctcca
    15421 gcgattgaaa tttatccgcc atgccagaca actaggtttc agtctggagt cgatccgcga
    15481 gttgctgtcg atccgcatcg atcctgaaca ccatacctgt caggagtcaa aaggcattgt
    15541 gcaggaaaga ttgcaggaag tcgaagcacg gatagccgag ttgcagagta tgcagcgttc
    15601 cttgcaacgc cttaacgatg cctgttgtgg gaccgcccat agcagtgttt attgctcgat
    15661 tcttgaagct cttgaacaag gggcgagtgg cgttaagagt ggctgttgat tttttgcact
    15721 gacaggatta cactcgcgcc gttaaataac caactggagt ttttatgagc cgatatcagc
    15781 atactaaagg gcagataaag gataatgcga tagaagcatt actacatgat cccttattcc
    15841 gacagcgagt agagaaaaat aagaaaggga aaggcagtta catgcgaaaa ggaaaacatg
    15901 gcaatcgggg gaactgggag gccagtggca agaaagtaaa tcactttttt accactggtc
    15961 ttctgctttc aggcgcttgt taagagcggt tattctgctc tttcagcaaa tcacgaattt
    16021 ctgccagtaa tacttcttct ttagttggtg caggtgcggc agctggttct tcttttttcc
    16081 gattcagttt gttgattagc ttaatcgcca taaagatggc aaaggccaca atcagaaaat
    16141 caaagacgtt ttgaatgaag acaccgtaat gcatcacaac agcagggata tccccctgcg
    16201 catcgcgtag cgtgacagca aactgtttaa agtcgatccc gccaattaat aagcccagag
    16261 gtggcatgat gatatcggca accagtgaag atacaatctt cccgaatgcc gcaccgataa
    16321 tgacacccac cgccaaatcc accacgttcc cgcgcatcgc aaattcgcga aattctttaa
    16381 taatgctcat gttattctcc ctatgaagcc gacaaccata agtctaacaa atgttaagcc
    16441 attttcctgc caggaatgaa tttaattaat ccttgataat caaagggaaa taataaggcg
    16501 tcattagacg ccttattaat tacaagaaga aagggcttgg ctggaagagt ctttcgacgt
    16561 cggtaataaa ctttttatct gtgaggaaca taattacgtg atcgccttgc tcaatgcgca
    16621 gattgtcatt ggcaatcatc acgtcgtttc cacgtaccac cgctccaata atcgttcctg
    16681 gcggtagctt gatttcgtca atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt
    16741 gagcaacggc ttcaatagct tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat
    16801 ctgcttttcg cacatggcta agcaacgcag aaatagttgc ttgttgtggt gaaattgcaa
    16861 tatcgatcac gctcccctga accagatcca cataagcgcg acgctggatc aataccatca
    16921 cctttttcgc acccatacgt ttggcaagca tggcggacat gatattggcc tcgtcatcgt
    16981 tggtgacggc aataaacaga tcaacttgat cgatatgttc ttcggccagc agttcttgat
    17041 ccgacgcatc accaaaaaag acgatcgtat tctgcaactt ttccgccagt tcggcagcgc
    17101 gctgctgatt acgttcgatg agtttaacgc tgtaatcttt ttccagacga cgcgccagcc
    17161 ctgcaccgat attaccgcca ccaaccagca tgatccgctt atacggtttt tccagtcgct
    17221 gtaattcact catcaccgcg cggatatgct gtgaagcggc aataaagaac acttcatcac
    17281 cagcttcaac aatggtcgaa ccttgtggac gaatggggcg atcgtggcgg aaaattgctg
    17341 ccacacgagt atcgatatgt ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca
    17401 gcgggccgcc ataataggct ttaactacag cgaggctgac tttaccctca gcgaagttca
    17461 ccacctgcaa tgcgcctgga tactcaatca gtcggtaaat attatcgata accaactgct
    17521 ctggtgcgat cagatgatca atcggtacag catctgaatg aaatagctta tcggcatcgc
    17581 gtacgtagtc tggtgagcgg atgcgagcga tgcgattagg ggtgttgaaa agtgagtagg
    17641 ctacctggca ggcaaccata ttggtttcat ctgaactggt tacagcaacc agcatatcgg
    17701 cgtcgtcggc acctgcctcc cgcaatacgc gtggatgaga gccatgcccc tgcacgactc
    17761 gcaggtcaaa tttatcctgc aaggtccgca gacgctcacc gttggtatcg acaacagtaa
    17821 tatcgttgtt ctcgccaacc aggttttccg ccagtgtgcc gccaacctgg ccggcaccca
    17881 gaatgataat tttcatcagt ctcgacccgt tatctcatca ctttttgatt agcttagcgt
    17941 aaaagaagcc gtcgccctct tcggcaccag gcagattttg tttacccggt tgctctggtg
    18001 ttcctgtttc gcaaagttcg gcatcagcgg tacgttgcaa aaaagcttta atctgcaggc
    18061 tattctcttc cggtaacacc gaacaggtgg catagaccag ggttccaccg gattttaaat
    18121 gcggccaaat ggcgtcgaga atttcagact gcaattgcgc gagttccggg atatcgcgat
    18181 cgcgacgtaa ccatttaata tccggatggc gacgaatcac ccccgttgct gaacaaggcg
    18241 catctaataa aatgcgatca aactgttgct cgccacacca ttgggaaggg taacggccat
    18301 caccttgttt cacggtcgcc ttcataccaa ggcgttttaa attgtcgtga acgcgagaga
    18361 ggcgctgttc gtcaatatca accgcaacaa cctgcgcttc tggtgccacc tcaaggatat
    18421 gcgttgtttt accgccgggg gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg
    18481 caagccaggt catgcaacct tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa
    18541 aaccaggtag cgcatgaaca ggtgcaggtg tttccagccg tacagcatca gggtaatccg
    18601 catgcgggaa acctttcatt cctgcttcat ccaacaatgc aagccagctg tcgcgggaat
    18661 gatgcgtacg attaacgcgc agccacattg gcggacgctg gttattggct tcgacgatgg
    18721 attgccactg ctctggatac gctttttgca gacgcttcag caaccaggaa ggatgcagat
    18781 aacgtgcatc actggtatta aactcggcta ataactcttc ttgctgacgc tggaactggc
    18841 gtaatacacc gttaatcaac cctttaagtt gcggacgctt aattgcgata gcgccttcaa
    18901 ccgtttcagc cagcgcagca tgaggtggaa tgcgggtata aagcagttga tacaaaccaa
    18961 ccataatcag gtaatgcaca gtgcgctgtt tgccggtcat cggacgggcc attaacttat
    19021 taatcagcca gtctaactgc gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa
    19081 gtgctttgtc tttatcagaa actttttgct ggagcggtgg cagaatgttg cttaatgatt
    19141 gcccttgctc gacgacttgt tcaacggcct gggccgccat gctacgtaaa ttacgttgtt
    19201 ttttcataag tataaaaatg cccggtaaga ccgggcttag aagagtggac tatcaggcca
    19261 gacggttgcc cggaacaaac cattcccgac gagaattcag gaggtcttgc gcgctcatcg
    19321 ctttcttacc cgcaggttgt aacgagagca ggttcaggat gccatcacca gtcgcaacct
    19381 gaatgccttg tttgttggct tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa
    19441 tgaccgacgc tttccagact ttaaccggtt gtccttcaat ttccagccag ctcattggcc
    19501 atgggttgaa agcgcgaatg cagcgttcaa gctgcgctgc cgaaagcgac cagtcaatac
    19561 gcgcttcttc tttactcaac ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg
    19621 gtttcgccgt gccgtctgct agttgtttca acgtggtgat tagcccttgt gggccaagct
    19681 ctgccagctt gtcgtacagc gtaccactgg tatcttctgc agtaatcggg caggagagct
    19741 tatagagcat atcaccggtg tctaaaccga catccatttg cataatggtc acaccagttt
    19801 ctgcatcacc cgcccatagt gagcgttgga ttggtgcagc accgcgccag cgtggcagca
    19861 gtgaaccatg aacgttgata cagccaagac gcggcatctc cagcactgct ttaggcagaa
    19921 ttaagccata ggcgacgacg accataacat cagcctgcag atcggcgacc agttgctggc
    19981 tttcttgtgg acgcagggaa acaggttgaa aaacgggcag acctttttcc tcagccagaa
    20041 ctttaaccgg gctgggcatc agttttttac cgcgtcctgc cggtcggtct ggctgggtga
    20101 acacgccaac gacgttatga ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt
    20161 caggtgtacc cgcaaaaata atacgtagtg attctgacac gttagttctt atccttaagc
    20221 ccgggctttc agacgatcta gtttttcaac tttctgacga atacgttgtt gtttcagcgg
    20281 tgacagataa tccataaaca gtttgccgac caggtgatcc atctcatgct gaatacagat
    20341 ggctaacagc ccctctgctt ccagttcaaa tggtttaccg tcgcggtcaa gggcgcgaat
    20401 tttaactttc tctgcgcgcg gcactaaagc acgttgttca gggatcgaca ggcaaccttc
    20461 ttcaatgcct gtttcgccgc ttttttctaa aagctccgga ttgattaaca ccagccgttc
    20521 gtcacggttt tccgaaacat caataacaat gatacgttga tggatatcaa cctgggttgc
    20581 cgccaggcca ataccttctt ctgcgtacat cgtctcgaac atatcatcga cgatacgctg
    20641 tatttctgca ttcacttctt ctaccggttt agcaactttg cgaagccgct cgtccggaat
    20701 atgtaacact tgcaaaactg acataaatct ccagagatgt gttcaggggt tagaaagatt
    20761 atttcttcta ttctagacaa atccccctct gattgacagc atcactgacc aatcgcaaag
    20821 attgctaagg ctgcttatgg cagggagata aggatggtcg atacagaaat ttggctgcgt
    20881 ttaatgagta tcagcagctt gtacggcgat gatatggtcc gtatagctca ctggctggca
    20941 aaacagtcgc aaattgatgc ggttggattg cagcaaacag ggcttacatt gcggcaggca
    21001 caacgctttc tttcatttcc gcgaaagagt atcgaaagct cactttgttg gttggagcaa
    21061 cccaaccatc atttaatccc tgcggacagc gaattttatc ctcctcaact tctggtgacg
    21121 acagattacc ccggcgcact gtttgttgaa ggagaactgc acgcgctgca ttcatttcag
    21181 cttgccgtag tggggagtcg ggcgcattca tggtatggcg agcgatgggg acggttattt
    21241 tgcgaaactc tggcgacgcg tggagtgaca attacgagtg gactggcgcg tggaatcgat
    21301 ggtgtggcgc ataaagctgc cttacaggta aatggcgtca gcattgctgt attggggaat
    21361 ggacttaata ccattcatcc ccgccgccat gcccgactgg ctgccagtct gcttgaacat
    21421 ggcggagctc tcgtctcgga atttcccctc gatgttccac cccttgctta caatttccca
    21481 cgaagaaatc gcattatcag tggtctaagt aaaggtgtac tggtggtgga agcggctttg
    21541 cgcagtggtt cgctggtgac agcacgttgt gcgcttgagc agaggcgtga agtttttgcc
    21601 ttgccaggtc caatagggaa tccgggaagc gaagggcctc actggttaat aaaacaaggt
    21661 gcgattcttg tgacggaacc ggaagaaatt ctggaaaact tgcaatttgg attgcactgg
    21721 ttgccagacg cccctgaaaa ttcattttat tcaccagatc agcaagacgt ggcattgcca
    21781 tttcctgagc tcctggctaa cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa
    21841 cgtgccggcc aacctgtgcc agaggtagtt actcaactac tcgaactgga gttagcagga
    21901 tggatcgcag ctgtacccgg cggctatgtc cgattgagga gggcatgcca tgttcgacgt
    21961 actaatgtat ttgtttgaaa cctatattca cactgaagct gagttgcgtg tggatcaaga
    22021 caaacttgaa caggatctta ccgacgcagg ttttgagcga gaagatatct acaatgccct
    22081 gctatggctg gaaaaacttg ctgattatca ggaagggttg gcagaaccga tgcaactggc
    22141 ctcggatcct ctctccatgc gtatttatac accggaagag tgtgaacgac tggatgccag
    22201 ctgccgtggg tttctgcttt tccttgagca gattcaggtg ctcaaccttg aaactcgtga
    22261 aatggtgata gagcgagtgc tcgcgctgga taacgcagag ttcgaactgg atgatctgaa
    22321 atgggtgatc ctgatggtgt tgttcaacat cccggggtgc gaaaatgcgt accagcaaat
    22381 ggaagaatta ctctttgaag tgaatgaagg tatgctgcat taatcatttc tttaattcag
    22441 cataagttgt tatggcaaaa tcagcactgt tcacggtgcg taataatgag tcctgcccaa
    22501 agtgcggggc tgaactggtt attcgatccg ggaaacacgg tccgtttctt ggatgctcac
    22561 agtatccggc gtgtgactac gtccgtcttc tgaaatcttc agcggatgga catattgtca
    22621 aagttctgga ggggcaggtt tgccctgcat gtggcgcaaa tctggtatta cgccagggac
    22681 gctttggtat gtttattggt tgcagtaact accctgaatg cgaacatacc gaacttatcg
    22741 ataaaccgga cgaaacagca attacatgcc cccaatgtcg gacgggccat ctggtccagc
    22801 gccgctcccg ttatggcaaa acatttcact cctgtgatcg ctacccggag tgtcaatttg
    22861 ccattaactt caaaccaata gctggagaat gccctgagtg tcattatccg ctactcatcg
    22921 aaaagaaaac cgcgcagggt gtaaaacact tttgtgccag taaacaatgt ggaaagccgg
    22981 tttcggcgga ataataacgt gaataataac ctgcaaggag acgctatcgc agctgcgata
    23041 gatgtcctca atgaagaacg tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg
    23101 tgcgatcctg atagcgaaac agcagtgatg agactgttgg agttaaaaca gcgtccggtt
    23161 gataaggggc tgattttaat cgcggcaaat tacgagcagc ttaaacccta tattgatgac
    23221 accatgctga ctgatgcgca gcgtgaaacc attttttccc gctggccagg tcctgtcacc
    23281 tttgtctttc ccgcgcctgc gacaacaccg cgctggttga cgggccgctt tgattcgctt
    23341 gctgtacgag tcaccgacca tccgttggtg gttgctttgt gccaggctta tggtaaaccg
    23401 ctggtttcta ccagtgccaa cttgagtgga ttgccacctt gtcgaacagt agacgaagtt
    23461 cgcgcacaat ttggcgcggc gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat
    23521 ccttcagaaa tccgcgatgc cctgacgggt gaactgtttc gacaggggta acataatgga
    23581 aacctatgct gtttttggta atccgatagc ccacagcaaa tcgccattca ttcatcagca
    23641 atttgctcag caactgaata ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga
    23701 tttcatcaac acactgaacg ctttctttcg tgctggtggt aaaggtgcga atgtgacggt
    23761 gccttttaaa gaagaggctt ttgccagagc ggatgagctt actgaacggg cagcgttggc
    23821 tggtgcagtt aataccctca agcggttaga agatggacgc ctgctgggtg acaataccga
    23881 tggtgtaggc ttgttaagcg atctggaacg tctgtctttt atccgccctg gtttacgtat
    23941 tctgcttatc ggcgctggtg gagcatctcg cggcgtacta ctgccacttc tttccctaga
    24001 ctgtgcggtg acaatcacta atcggacggt atcccgcgcg gaagagttgg ctaaattgtt
    24061 tgcgcacact ggcagtattc aggcgttggg tatggacgaa ctggaaggtc atgagtttga
    24121 tctcattatt aatgcaacat ccagtggcat cagtggtgat attccggcga tcccgtcatc
    24181 gctcattcat ccaggcattt attgctatga catgttctat cagaaaggaa aaactccttt
    24241 tctggcatgg tgtgagcagc gaggctcaaa gcgtaatgct gatggtttag gaatgctggt
    24301 ggcacaggcg gctcatgcct tccttctttg gcatggcgtt ctgcctgatg tagaaccggt
    24361 catcaaacta ttgcaacagg aattgtcagc gtgaatcagg ccatccagtt tccggatagg
    24421 gaagagtggg acgagaataa aagatgtgta tgttttcctg ctctcgtgaa tggtatgcaa
    24481 ctgacatgtg cgatctctgg cgagagtctg gcgtatcgct ttactggagg tacgccagaa
    24541 cagtggttag cgagttttcg tcagcatcgc tgggacctgg aagaagaagc ggaaaactta
    24601 attcaggaac aaagtgaaga tgatcaaggt tgggtctggt taccttgatc cagatattcg
    24661 tccttccatt tcacgtagtt atttgcggaa tagcgtaacc cagccttctc ttcatcactt
    24721 aacgggcgga tctgtttgac gggactaccg agatacagat atccgctctc cagccgttta
    24781 ttttgtggga ccaggctacc cgcaccaatc atcacatcat cttctactat tgcgccatca
    24841 agtaaaattg agcccatccc aaccaaaact cgattgccaa tggtgcagcc gtggagcatc
    24901 accttgtgac caacagtgac atcttcgcca atggttaatg ggttgccatc tgggttgtac
    24961 gaggatttat gagtgacatg caacatactg ccatcctgga tattggtgcg tgctccgatc
    25021 tgtacataat gtacatctcc acgaatcaca acgagcggcc agatccccac atcatcagcc
    25081 agacgaacgt caccaatcac gacgctgcta tcgtcgatca ttacgcgctg gccgatttgt
    25141 ggaaaaagat cgcggtatgg gcgtaaaaca tcagacatac tcacctcaac aataaatgat
    25201 ttcctaatga ctttgggtgc attattagcc ttgtgcaagt cttttagtat gcaaaaaagc
    25261 accgttttgt gtgcgaatgc agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc
    25321 aaaaaagtac ttgtgcaaaa aattgggatc cctataatgc gcctccgttg agacgacaac
    25381 gtgaaacact tcacaggatg gtcggaacaa cgaagagaaa aaattctgaa attcagggtt
    25441 gactctgaaa gaggaaagcg taatatacgc cacctcgcga cagtgagctg aaag
//
LOCUS       DAZPAC010000044        25105 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZPAC010000044 DAZPAC010000000
VERSION     DAZPAC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.044"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            194..382
                     /gene="bcsR"
                     /locus_tag="ACHUPM_001491"
     CDS             194..382
                     /gene="bcsR"
                     /locus_tag="ACHUPM_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR0869316.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            394..1146
                     /gene="bcsQ"
                     /locus_tag="ACHUPM_001492"
     CDS             394..1146
                     /gene="bcsQ"
                     /locus_tag="ACHUPM_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR0869317.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1143..1307
                     /locus_tag="ACHUPM_001493"
     CDS             1143..1307
                     /locus_tag="ACHUPM_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HFR0869318.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1314..3761
                     /gene="bcsA"
                     /locus_tag="ACHUPM_001494"
                     /pseudo
     CDS             1314..3761
                     /gene="bcsA"
                     /locus_tag="ACHUPM_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3772..6111
                     /gene="bcsB"
                     /locus_tag="ACHUPM_001495"
     CDS             3772..6111
                     /gene="bcsB"
                     /locus_tag="ACHUPM_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR0869319.1"
                     /translation="MKRKLFWICAVAMRMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6118..7224
                     /gene="bcsZ"
                     /locus_tag="ACHUPM_001496"
     CDS             6118..7224
                     /gene="bcsZ"
                     /locus_tag="ACHUPM_001496"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR0869320.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7206..10698
                     /gene="bcsC"
                     /locus_tag="ACHUPM_001497"
                     /pseudo
     CDS             7206..10698
                     /gene="bcsC"
                     /locus_tag="ACHUPM_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            10780..12768
                     /gene="hmsP"
                     /locus_tag="ACHUPM_001498"
     CDS             10780..12768
                     /gene="hmsP"
                     /locus_tag="ACHUPM_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR0869321.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12951..14237
                     /gene="dctA"
                     /locus_tag="ACHUPM_001499"
     CDS             12951..14237
                     /gene="dctA"
                     /locus_tag="ACHUPM_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR0869322.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14457..15953
                     /locus_tag="ACHUPM_001500"
     CDS             14457..15953
                     /locus_tag="ACHUPM_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0869323.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="ACHUPM_001501"
     CDS             complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="ACHUPM_001501"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR0869324.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17210..17977
                     /gene="pdeH"
                     /locus_tag="ACHUPM_001502"
     CDS             17210..17977
                     /gene="pdeH"
                     /locus_tag="ACHUPM_001502"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR0869325.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18047..20107
                     /locus_tag="ACHUPM_001503"
     CDS             18047..20107
                     /locus_tag="ACHUPM_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0869326.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20289..21611)
                     /locus_tag="ACHUPM_001504"
     CDS             complement(20289..21611)
                     /locus_tag="ACHUPM_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0869327.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="ACHUPM_001505"
     CDS             complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="ACHUPM_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR0869328.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23084..23983)
                     /locus_tag="ACHUPM_001506"
     CDS             complement(23084..23983)
                     /locus_tag="ACHUPM_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0869329.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24503..25105
                     /locus_tag="ACHUPM_001507"
     CDS             24503..25105
                     /locus_tag="ACHUPM_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR0869330.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5895 a         6365 c         6991 g         5854 t
ORIGIN      
        1 ttagcttgtg aatcaattta gaaggtcaca agacgtgtga ttttagtctt ttcgttttta
       61 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
      121 aataaaccgg cgaaatgctc acggttagaa ttgtttcatg aacggtacgg ttatttcata
      181 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
      241 tatcttccag aatgatattg tggcgttaaa gcaggcattt tcactgcctg atattgatta
      301 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
      361 agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg
      421 cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg
      481 ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct gtcatttaat
      541 gttgatttta cccaccgtca gggctgggcc agagcgatgc tggatggcca ggactggcgt
      601 gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt
      661 gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta
      721 cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc
      781 tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat cgtcaatgtg
      841 gatgccaact gccatatccg actgcatcag caagcgctgc cggatggcgc acatattttg
      901 attaatgact tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa
      961 agccagcgcc gattactgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg
     1021 gcggctaagc aaccagtagg tgaatatcgc agtgatgcgc tggcggctga agagatactg
     1081 acgctggcga actggtgcct gttgaactac tccgggctga aaacgccagt cgggagtgca
     1141 tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc
     1201 gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc ggctgtttct
     1261 ggatgatcct ggcctggatt tttattccgc tggagcaccc gcgctagcag cgtattcgcg
     1321 cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg ctggacccgg
     1381 tccgttatct cattcaaaca tgctggttac tgatcggtac atcgcgcaaa gaaacgccga
     1441 aaccgcgcag gcgggcattt tcaggtctgc agaatattcg tggacgttac catcaataga
     1501 tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag aaaaaagagc
     1561 tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc
     1621 tgattctggc gttaatctgc gttactcaac cgtttaaccc gctggcgcag tttatcttcc
     1681 tgatgctgct gtggggtgta gcgctgatcg tacggcggat gccggggcgc ttctcggcgc
     1741 taatgttgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg cgttacacct
     1801 ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg
     1861 aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc
     1921 gtcagccggt gccattgccg aaggatatgt cgctgtggcc gtcagtggat atctttgtcc
     1981 cgacttacaa cgaaaatctc aatgtggtga aaaataccat ttacgcctcg ctgggtatcg
     2041 actggccgaa agacaaactg aacatctgga tcctcgatga cggcggcagg gaagagtttc
     2101 gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg caccactcat gaacatgcga
     2161 aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg
     2221 actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag
     2281 agaaacagct ggcgatgatg cagactccac accacttctt ctcgccggac ccgtttgaac
     2341 gcaacctggg acgttttcgt aaaacgccga acgaaggcac gctgttctat ggtctggtgc
     2401 aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctatgcg gtgattcgtc
     2461 gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata
     2521 cttctctgcg tttgcaccgt cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg
     2581 cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg
     2641 ggatggtaca aatcttccgt ctcgataacc cgctcaccgg taaaggactg aagtttgctc
     2701 agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca cggctgatct
     2761 tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat gcgccagcgt
     2821 tgatgatcgc cctattcgtg ctgccgcata tgatccatgc cagcctgacc aactcgaaga
     2881 tccagggcaa atatcgccac tctttttgga gtgaaatcta cgaaacggtg ctggcgtggt
     2941 atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa tttaacgtca
     3001 ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg cggccctaca
     3061 tcttccttgt tctactcaac ctggtgggcg ttgcggtagg catctggcgc tacttctatg
     3121 gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtgttc tacaacctga
     3181 ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc
     3241 gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta
     3301 ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag gcgcagattc
     3361 tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac gtcttcccga
     3421 cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc
     3481 agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc
     3541 aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg aagctcggct
     3601 tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc atattccgtg
     3661 tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccacccg gagcggagcg
     3721 aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc gatgaaaaga
     3781 aaactattct ggatttgtgc agtggctatg aggatgagtg cgttcccctc tttcatgacg
     3841 caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc cgcccagacg
     3901 gaacaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgctga tgcgccagtc
     3961 gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat tgcgccgccg
     4021 ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt tggtatgcgc
     4081 agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc gccatcgtta
     4141 ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg cgtgctgcca
     4201 gtgaccaaag aacagttggg taaaaaaacg ctggcgcaaa tgcccattaa cccactgttt
     4261 attaccgact tcaaccgtgt gcggctggag tttgtcggcc attatcagga cgtgtgcgaa
     4321 aacccggcca gcaccacgct ttggctggat gttgggcgga gcagtggact ggatctgacc
     4381 tatcagaccc tgaatgtgaa gagtgacctg tcacacttcc cggtgccatt ctttgacccg
     4441 cgcgataacc gtactaacac cttgccgatg gtctttgcgg gtgcgccgga tgttgggctg
     4501 caacaagcat ctgccattgt cgcctcgtgg tttggttcgc gttctggctg gcgtgggcag
     4561 aacttcccgg tgctctataa ccaattgccg gatcgcaatg ccattgtctt tgcaaccaac
     4621 gacaaacggc cggacttcct gcgcgatcat ccggcggtaa aagccccggt gattgagatg
     4681 attaaccatc cgcagaatcc ttacgtcaaa ctgctggtgg tgtttggtcg tgacgacaaa
     4741 gacctgttgc aggcagcgaa aggtatcgct cagggtaaca ttctgttccg tggtgaaagc
     4801 gtggtagtga atgaagtgaa accgctgcta ccgcgtaagc cgtacgatgc gccgaactgg
     4861 gtacgtaccg atcgtccggt cacatttggc gaactgaaaa cctatgaaga acagttacaa
     4921 tccagcggtc ttgagccagc agcgattaac gtttcgctaa acctgccgcc ggatctctac
     4981 ctgatgcgca gtaccggcat tgatatggat attaattacc gctacaccat gccgccggtg
     5041 aaagacagtt cgcggatgga tatcagcctg aataaccagt tcctgcaatc cttcaacctg
     5101 agcagtaaac aggaggcgaa ccgcctgctg ctgcggattc cggtattaca aggtttgctg
     5161 gatggcaaaa cggatgtctc tattccggcg ctgaaactgg gcgcgaccaa ccagctgcgc
     5221 ttcgactttg agtatatgaa cccgatgccg ggcggttcgg tggataactg tattaccttc
     5281 cagccggtgc agaatcatgt ggtgattggt gacgattcca ccatcgactt ctcgaagtat
     5341 taccacttca tcccgatgcc ggatctacgc gcctttgcta acgcgggctt cccgttcagc
     5401 cggatggcgg atctgtcgca aaccatcacc gtgatgccga aaacgcctaa cgaagcacag
     5461 atggaaacgt tgctgaatac tgttggtttt atcggcgcac agacgggctt cccggcgatt
     5521 aatctgacgg tgaccgatga tggcagcacc attcagggca aagatgccga catcatgatc
     5581 atcggtggta tcccggacaa attaaaagac gataagcaga tcgacctgtt ggtgcaggcg
     5641 accgaaagct gggtgaaaac accgatgcgc cagaccccgt tccccggcat tgtgccggac
     5701 gagagcgatc gtgcggcaga aacccagtca acgctgacct cttccggtgc gatggcggcg
     5761 gtgattggct tccagtcgcc gtataacgac cagcgcagcg tgattgcgct gctggcagat
     5821 agcccacgcg gttatgaaat gcttaacgat gcggtgaacg atagcggcaa acgcgccacc
     5881 atgttcggtt cggtcgcggt gatccgcgag tccggtatca acagcctacg tgttggcgac
     5941 gtttattacg taggtcatct gccgtggttc gagcgcttgt ggtatgcgct ggcaaaccat
     6001 ccgattctgc tggcggtgct ggcggcaatc agtgtgatat tgctggcatg ggtactgtgg
     6061 cgtctgctgc gaattattag tcgtcgtcgt cttaacccgg ataacgagta attgaagatg
     6121 aatgtgttgc gtagtggaat cgtgacgatg ctgctgttgg ctgcctttag tgttcaggca
     6181 gcctgtacct ggcctgcctg ggagcagttt aaaaaggatt acatcagtca ggaagggcgc
     6241 gtcatcgccc ccagcgacgc gcgcaaaatc accacctccg aagggcaaag ttacggtatg
     6301 ttctttgccc tggcggctaa cgaccgtgta gctttcgata atattctcga ctggacgcag
     6361 aacaatctcg ctcagggctc tttaaaagaa cgtttgcccg cctggctgtg gggcaagaaa
     6421 gagaacagta agtgggaagt gctggacagc aattcggcct ccgatggtga tgtctggatg
     6481 gcctggtcgt tgctggaggc ggggcgtttg tggaaagagc agcgttatac cgacatcggc
     6541 agcgcgttgc taaaacgtat cgcgcgggag gaagtggtga cggtgcctgg gctgggttcc
     6601 atgttgttac cgggcaaagt gggttttgct gaggataaca gctggcgttt taaccccagc
     6661 tacctgccgc cgacgctggc gcagtatttc acccgctttg gcgcgccgtg gaccacgctg
     6721 cgcgaaacca atcaacgttt attgctggaa accgccccga aaggtttttc gccagactgg
     6781 gtgcgctatg agaaagacaa aggctggcag ctaaaagccg aaaaaacatt gatcagcagc
     6841 tacgacgcta tccgcgttta catgtgggta ggcatgatgc ctgacagcga tccgcaaaaa
     6901 gcgcggatgc tcaaccggtt taaaccgatg gcgacattca ctgagaaaaa cggttatccg
     6961 ccggaaaaag tggatgtggc tacggggaaa gcgcagggta aaggaccgat cggtttttct
     7021 gccgccatgc tgcccttttt acaaaaccgc gatgcgcagg ccgttcagcg ccagcgcgtg
     7081 gccgataact ttcccggcag cgatgcctat tacaactatg tgctgaccct gtttggacaa
     7141 ggctgggatc aacaccgttt ccgcttctcg acaaaaggtg agttattacc tgactggggc
     7201 caggaatgcg caaattcaca ctaaacatat tcacgctttc cctcggtctg gccgtcatgc
     7261 cgatggtcga ggcagcacca accgctcagc aacagttgct ggagcaagtt cggttaggcg
     7321 aagcgaccca tcgtgaagat ctggtgcaac agtcgttata tcggctggaa cttattgatc
     7381 cgaataaccc ggacgtcgtt gccgcccgtt tccgttcttt gttacgtcag ggcgatattg
     7441 atggcgcgca aaaacagctc gatcggctgt cgcagttagc gccgagttca aatgcgtata
     7501 aatcgtcgcg gactacgatg ctactttcca cgccggatgg tcgtcaggca ctgcaacagg
     7561 cacgattgca ggcgacgacc ggtcatgcag aagaagctgt ggcgagttac aacaaactat
     7621 tcaacggtgc gccgccggaa ggtgacattg ctgtcgagta ctggagtacg gtggcgaaaa
     7681 ttccggctcg ccgtggcgaa gcgattaatc agttaaaacg catcaatgcg gatgcaccgg
     7741 gcaatacggg cctgcaaaac aatctggcgc tattgctgtt tagtagcgat cgccgtgacg
     7801 aaggttttgc cgtcctggaa cagatggcaa aatcgaacgc cgggcgcgaa ggggcctcta
     7861 aaatctggta cgggcagatt aaagacatgc ccgtcagtga tgccagtgtg tcggcgctga
     7921 aaaaatatct ctcgatcttt agtgatggcg atagcgtggc ggctgcgcaa tcgcaactgg
     7981 cagaacagca aaaacagctg gccgatcctg ctttccgcgc tcgtgcgcaa ggtttagcgg
     8041 cggtggactc tggtatggcg ggtaaagcca ttcccgaact acaacaggcg gtgcgggcga
     8101 acccgaaaga cagtgaagct ctgggggcgc tgggccaggc gtattctcag aaaggcgatc
     8161 gcgccaatgc agtggcgaat ctggaaaaag ccctcgcact ggacccgcac agcagcaaca
     8221 acgacaaatg gaacagtctg ctgaaagtaa accgctactg gctggcgatc cagcagggcg
     8281 atgctgcgct gaaagccaat aatcctgacc gggcagaacg cctgttccag caggcgcgta
     8341 atgtcgataa caccgacagt tatgcagtgc tggggctggg cgatgtggcg atggcgcgaa
     8401 aagattatcc cgccgccgaa cgttattatc agcagacctt gcgtatggac agcggcaaca
     8461 ctaacgccgt gcgcgggctg gcaaatattt accgccagca atcgccagaa aaagctgaag
     8521 cgtttatcgc ctcgctctct gccagtcagc ggcgtagcat tgatgatatc gaacgcagcc
     8581 tgcaaaacga ccgtctggca cagcaggcag aggcactgga aaaccagggc aaatgggcgc
     8641 aggcggcagc acttcagcgg caacgactgg cgctggaccc cggcagcgta tggattactt
     8701 accgactttc gcaggatctc tggcaggccg gacaacgcag ccaggccgat acgttaatgc
     8761 gcaatctggc gcagcagaag tcgaacgacc cagagcaggt ttacgcttac gggctgtacc
     8821 tacgcttacg ggctgtacct ctctggtcat gaccaggaca gagcggcgct ggcgcatatc
     8881 aacagcctgc cgcgtgcgca gtggaacagc aatattcagg agctggttaa tcgactgcaa
     8941 agcgatcagg tgctggaaac cgctaaccgc ctgcgagaaa gcggcaaaga ggcagaagcg
     9001 gaagcgatgc tgcgccagca accaccttcc acgcgtattg acctcacgct ggctgactgg
     9061 gcgcaacaac gacgtgatta caccgccgcc cgcgctgcat atcagaatgt cctgacgcgg
     9121 gagccagcta acgccgacgc cattcttggt ctgacggaag tggatattgc tgccggtgac
     9181 aaagcggcgg cacgtagcca gctggcgaaa ctgcccgcta ccgataacgc ctcgctgaac
     9241 acacagcggc gcgtggcgct ggcacaggcg cagcttggcg ataccgcagc agcgcagcgg
     9301 acgtttaata agttgatccc gcaggcaaaa tctcagccac cgtcgatgga aagcgcgatg
     9361 gtgctgcgtg atggtgcgaa gtttgaagcg caggcgggcg atccaacgca ggcgctggaa
     9421 acctacaaag acgccatggt cgcatccggt gtgactacga cgcgtccgca ggataacgac
     9481 acctttaccc gactgacccg taacgacgag aaagatgact ggctgaaacg tggcgtgcgc
     9541 agcgatgcgg cggacctcta tcgccagcag gatcttaacg tcacccttga gcacgattac
     9601 tggggttcga gcggcaccgg tggttactcc gatctgaaag cgcacactac catgttgcag
     9661 gtggatgcgc cgtattctga cgggcggatg ttctttcgca gtgatttcgt caatatgaac
     9721 gtcggcagtt tctccactaa tgccgatggc aaatgggatg acaactgggg cacctgtaca
     9781 ttacaggact gtagcggcaa ccgcagccag tcggattccg gtgccagcgt ggcggtcggc
     9841 tggcgaaatg acgtctggag ctgggatatc ggtaccacgc cgatgggctt caacgtggtg
     9901 gatgtggtcg gcggcatcag ttacagcgat gatatcgggc cgctgggtta caccgttaac
     9961 gcccaccgtc ggcccatctc cagttctttg ctggcctttg gtgggcaaaa agactccccg
    10021 agcaataccg ggaaaaaatg gggtggcgta cgtgccgacg gtgtggggct aagtctgagc
    10081 tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta gtggcgacca gttaaccggt
    10141 aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg gctattacta taaggtcatt
    10201 aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca tgatctggca ttacgacaaa
    10261 gatctgagtg gctactcact cggtcagggc ggttactaca gtccgcagga atacctgtcg
    10321 tttgccatac cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga gctgggggcg
    10381 tctggctcgt ggtcgcattc acgcaccaaa accatgccgc gttatccgct gatgaatctg
    10441 atcccgaccg actggcagga agaagctgcg cggcaatcca acgatggcgg cagcagtcag
    10501 ggcttcggct acacggcgcg ggcattactt gaacgacgtg ttacttccaa ctggtttgtt
    10561 ggcacggcaa ttgatatcca gcaggcgaaa gattacgcac ccagccattt cctgctctac
    10621 gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca gccgctgata
    10681 ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg cgatcgggta
    10741 tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag tcgctcgtta
    10801 acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt tattttttgc
    10861 accgttttgc tgttccatct ggtccagcag aatcgctata acacggctac gcaactggaa
    10921 agcattgctc gctctgtccg cgaaccctta tcttcagcta ttttgaaagg cgatattccc
    10981 gaagcggaag ctattcttgc cagcattaaa ccggcaggcg tggtcagccg tgccgatgta
    11041 gtgctgccta accagttcca ggcgctgcgt aaaagtttta ttccagagcg cccggtgccg
    11101 ataatggtta ctcgcctgtt tgagctaccg gttcaaatct cgctgggcgt ttactcgctc
    11161 gaacgtccgg caaacccgca gccaattgcc tatctggtac tacaggcgga ttccttccgt
    11221 atgtataagt tcgtgatgag caccctctca acgttagtga ccatttactt acttttgtcg
    11281 cttatcctga ccgtcgccat cagctggtgc attaaccgcc tgattttgca tccgttacgc
    11341 aatattgctc gcgaacttaa cgccatccca gcccaggagc ttgttggtca ccaactggca
    11401 ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta caacctcaac
    11461 cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg cttcccggtg
    11521 tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggttgt cgcgcgtaaa
    11581 caaaccaccg cgctgatgat catcacctgt gaaaccctgc gtgatactgc gggcgtgctg
    11641 aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa atcggtactg
    11701 tcgccacgta tgatcctcgc gcagattagc ggttatgact ttgctgtcat tgccaacggt
    11761 gtacaggaac cgtggcacgc aatcacctta ggtcagcaag tgctcaccat catgagcgag
    11821 cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattggcgt ggcgatgttc
    11881 tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc atttaccgct
    11941 cgccataaag gcaagaatca gattcagttc tttgatccgc agcagatgga agccgcccag
    12001 aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca gtttgctatt
    12061 tggttacagc cacaggtcga gatgaccagc ggtaaactgg tcagtgcgga agtgttactg
    12121 cgtatccagc aaccggatgg cagttgggac ctgccggatg gcttaatcga tcgcattgag
    12181 tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg tcgattgctt
    12241 gcagcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc tgcgctgcaa
    12301 ctgatgcacc caaatatggt ggcggatatg ctggaactgt taacccgcta tcgcattcag
    12361 ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgaccc tcatgctgcg
    12421 gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga tgatttcggc
    12481 atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat cgacgtactg
    12541 aaaatcgaca aaatgtttgt tgaaggcttg ccggaagata gcagcatgat tgctgcaatt
    12601 atcatgctgg cgcagagcct gaacttacaa atgattgccg aaggcgtgga gactgaagca
    12661 caacgcgact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct ttttgctcac
    12721 ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct accccaaact
    12781 gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt ttaacctttt
    12841 tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat tttaaggccg
    12901 caggtacccc ataaccttac aagacctgtg gttttactaa aggacaccct atgaaaacct
    12961 ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt attctccttg
    13021 gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc ttcgttaagc
    13081 tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc attgcgggca
    13141 tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac tttgaaattg
    13201 tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag cctggtgccg
    13261 gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac gccgatcagg
    13321 cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc gtcattggcg
    13381 catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt ggttttgcgc
    13441 tccaccgtct gggcagcaaa ggccaactga tttttaacgt catcgaaagt ttctcgcagg
    13501 tcatcttcgg catcatcaat atgatcatgc gtctggcacc tattggtgcg ttcggggcaa
    13561 tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg cagctgatta
    13621 tctgtttcta tattacctgt atcctgtttg tggtgctggt attgggttca atcgctaaag
    13681 cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg ctgattgtac
    13741 tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg gagaaactcg
    13801 gctgccgcaa atcggtggtg gggctggtca tcccgacagg ctactcgttt aaccttgatg
    13861 gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact aacagtcaga
    13921 tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct aaaggggcgg
    13981 caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg gtgggccatt
    14041 tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca gaagctcgtg
    14101 cgctgactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag tgggtgaaag
    14161 aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat ggcaaaacgc
    14221 acgaattatc ctcttaatct caccacttat gcccgcactc ccttccccga gtgcgggtgt
    14281 ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag gtttatttca
    14341 cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt aacgtcattt
    14401 agactgactg acaacgggca gttttttatt accaggatag ttgatcaggg gttcacatgc
    14461 agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    14521 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    14581 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    14641 tggttaatat cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    14701 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    14761 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    14821 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc
    14881 tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa
    14941 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    15001 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    15061 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg
    15121 tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg tttggcgaac
    15181 tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag
    15241 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    15301 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    15361 gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagc aaagacatcg
    15421 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    15481 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    15541 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    15601 tgcagaaact gtttgccgcc tatgcgcggg ctgataccga tattctgatg ggtcagcgga
    15661 tgcgttcgct gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    15721 aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt cagcagttgt
    15781 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    15841 cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgctgctgcc gccacctctg
    15901 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    15961 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac
    16021 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    16081 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg
    16141 cactgccgcc cgtcaaacat accgccagat aaccggcgct gaaagagtcg ccagctgcgg
    16201 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatccact aacccttcgc
    16261 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    16321 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcat
    16381 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac acctgctgtg
    16441 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    16501 cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    16561 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    16621 ccgcagactg ctcactctcc agccagaatt tggcggcggc ttcgttccgc cagtagtaga
    16681 acgtacgctc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga cggttttcca
    16741 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atttgctggc
    16801 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    16861 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    16921 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    16981 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    17041 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    17101 ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgcccttg cgcgccgata
    17161 atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca
    17221 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    17281 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcggacggtt
    17341 aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc
    17401 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    17461 gattgatttg ctggcgcaaa aagccgactt ctttgtagag cacgacctgc tggcatcggt
    17521 caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccaaat
    17581 tgagcgtctt ccctggctgc gtttcgaact ggtggaacac atccgcctgc cgaaagattc
    17641 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gcaccgggat
    17701 ggcaaatttc tctgcgctaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagagct
    17761 gttcgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc ttttacatct
    17821 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg aagagtggcg
    17881 tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    17941 gatggaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgctgggct
    18001 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    18061 caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt
    18121 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    18181 gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaaaca
    18241 agaaactggc tggcgcagct gggtgccgtg gcctcatgta catgcagaag acatcattct
    18301 tggtaatcca ccggatattc ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac
    18361 gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggttca agctcgaaaa
    18421 gcccgacgcg cgcctgattc gcctctccga aaagaacaat aactggacgt ttaatcttgc
    18481 caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    18541 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    18601 aatttttgtt gatccgttag gcaagccgct gccgttcagc gaagttactg gatcgaaagg
    18661 taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc agggacgtta
    18721 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    18781 agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga
    18841 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    18901 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    18961 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    19021 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    19081 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    19141 cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga
    19201 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    19261 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    19321 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    19381 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcga cgaatattca
    19441 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    19501 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    19561 tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca acagtaacgg
    19621 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    19681 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    19741 ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga tttttgcttt
    19801 cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt cagaacagct
    19861 ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct
    19921 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac cgctgattgc
    19981 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccgacggcgg cgttactggc
    20041 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    20101 gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt agatttttgg
    20161 caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct
    20221 tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca tcaggctttt
    20281 cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc
    20341 aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt agtctgcaac
    20401 caggctgcga tatacggtgc aacggaagcc ccgagaatcg acgctacgtt gtaagagaat
    20461 gatgctccgg tgtaacgcac ttctgttggg aacagctctg gtaacagcgc gcccatcggc
    20521 ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg
    20581 ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg
    20641 ataattacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc gactggcacc
    20701 atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt acgcggcagg
    20761 ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt catgatgtaa
    20821 aacagcgtat aggttgccag catgatgaac gtacccagta cggttacgcg aacatgtttg
    20881 gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac
    20941 accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag taccgccgag
    21001 aagataaatg gcacacgcca gccccagctc ataaactgct cgtcggtcag tagccaggaa
    21061 agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcccag ctgtgggaag
    21121 gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg
    21181 ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagcggg
    21241 gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt tgaaatcccc
    21301 atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca
    21361 aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt
    21421 agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac aataacggcc
    21481 gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca
    21541 aggactttat tacgcgagtt gatcggcgtg tattcttgct cgtggtcgag tgttgtggct
    21601 gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa
    21661 aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga
    21721 taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg
    21781 gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg
    21841 agttggaatg aaattgtttg ctcccaaata gatggctgaa gaggctctgg ggaagagttt
    21901 caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat ccggcctacg
    21961 gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca tccggcctat
    22021 tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc
    22081 acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc agccccagca
    22141 cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg taggtcatca
    22201 cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc agggcttttt
    22261 tgcgcggacg gtggcgcggt aaccgccaga agatccagaa gaaaagcaga tagttggcga
    22321 agatggaaat cgccaggcca atcaaccgcc acgtcggttt cagccactca atgctgttca
    22381 ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg
    22441 tcacaatcag cgcaatcaac aaaccaatca gcgaaataaa atcacgtaga tattttaccc
    22501 agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg gcgcgaatcg
    22561 cttcacgcag gttacccatc cagttgatgc cggaataaag cgccacagcc aggccgacaa
    22621 gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac gtggcggcta
    22681 gcgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc aacatcggat
    22741 gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg atcatcgaca
    22801 aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg ttaaagcgtt
    22861 ctgtcgcgcg aatcaggtgc gcaataaccg gttggcgttg gactttttcg gcagtggtgg
    22921 tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc aactgcttaa
    22981 tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc gtcatccgct
    23041 cttttgtcct tctgttgtgg tcattcttaa aaagtatagt cggtcagtcc aggtactctt
    23101 tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc tcccgacgct
    23161 gcggataaac cagcgaaagg gagagcggct cggcacggta gcctggcaac acttcaataa
    23221 gctgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata atccccagac
    23281 cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg ccgccagact
    23341 taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt acacccaggt
    23401 gcggtgtata acgcaccatt gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa
    23461 agcgcgtcag atagtgcgga ctggcacagt tgaccatggt cagtttgccg aggggacggg
    23521 cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa
    23581 gaatatctac cggacggtta tggctactca gttccagctc aattcccgga tgctgataga
    23641 gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc
    23701 gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt tcgctgagat
    23761 tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg agtttgaccc
    23821 gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc tgtatctggc
    23881 gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct
    23941 ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg accgattgtt
    24001 gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct aacatctaat
    24061 gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga aaagggcgga
    24121 atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt gattcccttt
    24181 aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg ccatttgtta
    24241 ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc accaatataa
    24301 ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg cgtttagctg
    24361 taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg ccaccgccaa
    24421 atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag tctttttaac
    24481 gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt caatatttat
    24541 tcatggaatg aaaatcagtt tacagcagcg tattccagga gtgagtattc agggggccag
    24601 tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag ttatgctcga
    24661 tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc
    24721 tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac aggaagttat
    24781 tcattttaat gtgctggcca ttgtgcctcg tgattcaacc gtcgagacat ttgcgttggc
    24841 ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga cgacgccgga
    24901 aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt taaccatgtt
    24961 agcggcgggc gaatcaaata aggagatcgg cagagcgctg aatatcagta ccggaacggt
    25021 aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc
    25081 aatgatgtta aatatctcct cctga
//
LOCUS       DAZPAC010000045        24537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZPAC010000045 DAZPAC010000000
VERSION     DAZPAC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.045"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(166..576)
                     /locus_tag="ACHUPM_001508"
     CDS             complement(166..576)
                     /locus_tag="ACHUPM_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR0869331.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(634..867)
                     /locus_tag="ACHUPM_001509"
     CDS             complement(634..867)
                     /locus_tag="ACHUPM_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFR0869332.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1256..1801
                     /locus_tag="ACHUPM_001510"
     CDS             1256..1801
                     /locus_tag="ACHUPM_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR0869333.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1776..3701
                     /locus_tag="ACHUPM_001511"
     CDS             1776..3701
                     /locus_tag="ACHUPM_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR0869334.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3698..3904
                     /locus_tag="ACHUPM_001512"
     CDS             3698..3904
                     /locus_tag="ACHUPM_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR0869335.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3901..5502
                     /locus_tag="ACHUPM_001513"
     CDS             3901..5502
                     /locus_tag="ACHUPM_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR0869336.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5483..6802
                     /locus_tag="ACHUPM_001514"
     CDS             5483..6802
                     /locus_tag="ACHUPM_001514"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR0869337.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6812..7144
                     /locus_tag="ACHUPM_001515"
     CDS             6812..7144
                     /locus_tag="ACHUPM_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR0869338.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            7200..8225
                     /locus_tag="ACHUPM_001516"
     CDS             7200..8225
                     /locus_tag="ACHUPM_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR0869339.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8267..8665
                     /locus_tag="ACHUPM_001517"
     CDS             8267..8665
                     /locus_tag="ACHUPM_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR0869340.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8677..9030
                     /locus_tag="ACHUPM_001518"
     CDS             8677..9030
                     /locus_tag="ACHUPM_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR0869341.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9042..9620
                     /locus_tag="ACHUPM_001519"
     CDS             9042..9620
                     /locus_tag="ACHUPM_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0869342.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9617..10012
                     /locus_tag="ACHUPM_001520"
     CDS             9617..10012
                     /locus_tag="ACHUPM_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR0869343.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            10020..10760
                     /locus_tag="ACHUPM_001521"
     CDS             10020..10760
                     /locus_tag="ACHUPM_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0869344.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10776..11198
                     /locus_tag="ACHUPM_001522"
     CDS             10776..11198
                     /locus_tag="ACHUPM_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR0869345.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            11180..11614
                     /locus_tag="ACHUPM_001523"
     CDS             11180..11614
                     /locus_tag="ACHUPM_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR0869346.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11607..14168
                     /locus_tag="ACHUPM_001524"
     CDS             11607..14168
                     /locus_tag="ACHUPM_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0869347.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14165..14494
                     /locus_tag="ACHUPM_001525"
     CDS             14165..14494
                     /locus_tag="ACHUPM_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0869348.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14494..15192
                     /locus_tag="ACHUPM_001526"
     CDS             14494..15192
                     /locus_tag="ACHUPM_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR0869349.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15198..15941
                     /locus_tag="ACHUPM_001527"
     CDS             15198..15941
                     /locus_tag="ACHUPM_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0869350.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15938..16510
                     /locus_tag="ACHUPM_001528"
     CDS             15938..16510
                     /locus_tag="ACHUPM_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR0869351.1"
                     /translation="MKTGAEAIRAMATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVMPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16571..20068
                     /locus_tag="ACHUPM_001529"
     CDS             16571..20068
                     /locus_tag="ACHUPM_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR0869352.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            20139..20655
                     /locus_tag="ACHUPM_001530"
                     /pseudo
     CDS             20139..20655
                     /locus_tag="ACHUPM_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20720..23803
                     /locus_tag="ACHUPM_001531"
     CDS             20720..23803
                     /locus_tag="ACHUPM_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0869353.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTLETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTAYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHQH
                     SQTGPRTNSGSQPTGMFPAGSTQVSGTNQVGISGSLTSGTSQWVGKSSSEGNHTHSLS
                     GTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            23815..24384
                     /locus_tag="ACHUPM_001532"
     CDS             23815..24384
                     /locus_tag="ACHUPM_001532"
                     /inference="COORDINATES: protein motif:HMM:NF014467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0869354.1"
                     /translation="MSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTDIAPP
                     DIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSPEGEF
                     QKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKETLLL
                     EAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT         5820 a         6115 c         7453 g         5149 t
ORIGIN      
        1 gtcgccgtat tcccgtatta acagagaccg tagcccgacg gggaactcct tctgcgcgag
       61 tgtgcgggaa taatcaaaaa cgatgcacac cggggttacc gggtacacat atttcatcat
      121 gccagcgagt ccggttctgg cacggaagaa accggacgtt atgatttagt gcggaaatat
      181 ttgtgtagtg ttctgtatgt tctcagtaaa gagtaatgaa ttatcaaagg tatagtaata
      241 ccttttgttt tcgtggatat ttgtaatcca tctgaaaacc cctgctgtag caagattttt
      301 cctgtattcg taaaatgata actctcctga tttgaatcct tttaaggtgg cttctataag
      361 gcatttattt tttgaaaatc ttacatttac aaccttaccc tgtcctttta ttaaaaccgt
      421 attatcgttt tcaagaacaa gatgaatatt ctctgtggct aaatagtaaa tgtaatgtga
      481 gacattgtga cgttttagtt cagaataaaa ccagtgatag tttaaattat ttcgcacttt
      541 atcgaatatt tgtttaaaaa tggcaacctg agccattgta gtaccttcca tgtgatatga
      601 gggggcgtag tctgcacgat tatctaaatt gcttcaatct ggtctaacct gttttctgag
      661 caattcagta atgtcactct tttctttgtt tgcttcaggc gaaactcttt tttctgagca
      721 cagtctccgg cggcaggctt caatgaccca ggctgagaaa ttcccggacc ctttttgaac
      781 aagagcgatg ttaatttgtt caatcatttg gttaggaaag cggatgttgc gggttgttgt
      841 tctgcgggtt ctgttcttcg ttgacatgag gttgccctgt attcagtgtc gctgatttgt
      901 attgtctgaa gttgttttta cgttaagttg atgcagatca attaatacga tacctgcgtc
      961 ataattgatt atttgacgtg gtttgatggc gtagatgcac gttgtgacat gtagatgata
     1021 attattatca ttttgcgggt cctttccggc gatccgacag gttacggggc ggaaggcgcg
     1081 cgggttttcg ctatttatga caattttccg gtttaaggcg tttccgttct tcttcgccgt
     1141 aacctaatgt ttttatttaa aacaccccct gaaaagaaag gaaacgacag gtgctgaaaa
     1201 cgggcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat gaacaatgga
     1261 agtcaacaaa aagcagctgg ctgacatttt cggtgcgagt atccgtacca ttcagaactg
     1321 gcaggaacag ggaatgcccg ttctgcgagg cggtggcaag ggtaatgagg tgctttatga
     1381 ctctgccgcc gttataagat ggtatgccga aagggatgct gaaattgaga acgaaaagct
     1441 gcgccgggaa gttgaagaac tgcggcaggc cagcgagaca gatctccagc cagggactat
     1501 tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac gcacaggagc tgaaaaatgc
     1561 cagagactcc gctgaagtgg tggaaaccgc attctgtact ttcgtgctgt cgcggatcgc
     1621 aggtgaaatt gccagtattc tcgacgggat ccccctgtcg gtgcagcggc gttttccgga
     1681 actggaaaac cgacatgttg atttcctgaa acgggatatc atcaaagcca tgaacaaagc
     1741 agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac agtcaggtta
     1801 acaggctgcg gcattttgtc cgcgccgggc ttcgctcact gttcaggccg gagccacaga
     1861 ccgccgttga atgggcggat gctaattact atctcccgaa agaatccgca taccaggaag
     1921 ggcgctggga aacactgccc tttcagcggg ccatcatgaa tgcgatgggc agcgactaca
     1981 tccgtgaggt gaatgtggtg aagtctgccc gtgtcggtta ttccaaaatg ctgctgggtg
     2041 tttatgccta ctttatagag cataagcagc gcaacacact tatctggttg ccgacggatg
     2101 gtgatgccga gaactttatg aaaacccacg ttgagccgac catccgcgat attccgtcgc
     2161 tgctggcgct ggctccgtgg tatggcaaaa agcaccggga taacacgctc actatgaagc
     2221 gtttttccaa tggtcgtggc ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg
     2281 aaaagtcggt ggatgtggcg ggttatgatg aacttgctgc ctttgatgag gatattgaac
     2341 aggaaggctc tccgacgttc cttggcgaca aacgtattga aggctcggtc tggccaaagt
     2401 ccatccgtgg ctccacgccc aaagtgagag gcacctgcca gattgagcgt gcagccagtg
     2461 aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtatc
     2521 ttaaatttgg cgacaaagag acgccgtttg gcctcaaatg gacgccggat gacccctcca
     2581 gcgtgtttta tctctgcgag cataatgcct gcgtcatccg ccagcaggag ctggacttta
     2641 ctgatgcccg ttatatctgc gaaaagaccg ggatctggac ccgtgatggc attctctggt
     2701 tttcgtcatc cggtgaagag attgagccac ctgacagtgt gacctttcac atctggacag
     2761 cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaaa acgaaagggg
     2821 atacgggaaa acgtaaaacc ttcgtaaaca ccacgctcgg tgagacgtgg gaggcgaaaa
     2881 ttggcgaacg tccggatgct gaagtgatgg cagagcggaa agagcattat tcagcgcccg
     2941 ttcctgaccg tgtggcttac ctgaccgccg gtatcgactc ccagcgggat cgctacgaaa
     3001 tgcgcgtatg gggatggggg ccgggtgagg aaagctggct gattgatcgg cagattatta
     3061 tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc aataaaacct
     3121 atacccgccg gaatggtgca gaaatgtcga tatcccgtat ctgctgggat attggcggga
     3181 ttgacccgac cattgtgtat gaacgctcga aaaagcatgg gctgttccgg gtgatcccca
     3241 ttaaaggggc atccgtctac ggtaagccgg tggccagcat gcctcgtaag cgaaacaaaa
     3301 acggggttta ccttaccgaa atcggtacgg ataccgcgaa agagcagatt tataaccgct
     3361 tcacactgac gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc ccgaataacc
     3421 cggatatttt tgatctgacc gaagcgcagc agctgactgc tgaagagcag gtcgaaaaat
     3481 gggtggatgg caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat gaggcgctcg
     3541 actgcttcgt ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg cagctggatc
     3601 tcagtgcact gctggcgagc ctgcaggaag aggatggtgc agcaaccaac aagaaaacac
     3661 tggcagatta cgcccgtgcc ttatccggag aggatgaatg acgcgacagg aagaacttgc
     3721 cgctgcccgt gcggcactgc atgacctgat gacaggtaaa cgggtggcaa cggtacagaa
     3781 agacggacgg cgagtggagt ttacggccac ttccgtgtct gacctgaaaa aatacattgc
     3841 ggagctggag gtgcagaccg gcatgacaca gcgacgcagg ggacctgcag gattttatgt
     3901 atgaaaacgc ccaccattcc cacccttctg ggaccggacg gcatgacatc gctgcgtgaa
     3961 tatgccggtt atcacggcgg tggcagcgga tttggtgggc agttgcgggc gtggaaccca
     4021 ccgggtgaaa gtgtggatgc agccctgctg cccaacttta cccgtggcaa tgcccgcgca
     4081 gacgatctgg tacgcaataa cggctatgct gccaacgcca tccagctgca tcaggatcat
     4141 atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg
     4201 gaggaagacg cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag
     4261 gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa
     4321 ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt
     4381 tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg
     4441 aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg
     4501 gcgctgggat attacgtcag cgaggacggc tatcctggct ggatgccgca gaaatggaca
     4561 tggatacccc gtgagttacc cggcgggcgc gcctcgttca ttcacgtttt tgaacccgtg
     4621 gaggacgggc agacccgcgg tgcaaatgtg ttttacagcg taatggagca gatgaagatg
     4681 ctcgacacgc tgcagaacac gcagctgcag agcgccattg tgaaggcgat gtatgccgcc
     4741 accattgaaa gtgagctgga tacgcagtca gcgatggatt ttattctggg cgcgaacagt
     4801 aaggagcagc gggaaaggct gacgggctgg attggtgaaa ttgccgcgta ttacgccgca
     4861 gcaccggtcc gtctgggagg cgcaaaagtg ccgcacctga tgccggggga ctcactgaac
     4921 ctgcagacgg ctcaggacac ggataacggc tactccgtgt ttgagcagtc actgttgcgg
     4981 tatatcgctg ccgggctggg tgtctcgtat gagcaacttt cccggaatta cgcccagatg
     5041 agctactcca cggcacgggc cagtgcgaac gagtcgtggg cgtactttat ggggcggcga
     5101 aaattcgtcg catcccgtca ggcgagccag atgtttctgt gctggctgga agaggccatc
     5161 gttcgccgcg tggtgacgtt accttcaaaa gcgcgcttca gctttcagga agcccgcagt
     5221 gcctggggga actgcgactg gataggctcc ggtcgtatgg ccatcgatgg tctgaaagaa
     5281 gttcaggaag cggtgatgct gatagaagcc ggactgagca cctacgagaa agagtgcgcg
     5341 aaacgcggtg acgactatca ggaaattttt gcccagcagg tccgtgaaac gatggagcgc
     5401 cgcgcagctg gtcttaaacc gcccgcctgg gcggctgcgg catttgaatc cgggctgcga
     5461 caatcaacag aggaggagaa gagtgacagc agagctgcgt aatctcccgc atattgccag
     5521 catggctttt aatgagccgc tgatgcttga acccgcctat gcgcgggttt tcttttgtgc
     5581 gcttgcaggc cagcttggga tcagctgcct gacggatgca gtatccggcg acagcctgac
     5641 tgccggagag gcacccgcgg cgctggcgtt atccggtgat gatgacggac cacgacaggc
     5701 ccgcagttat caggtaatga acggcatcgc cgtgctgccg gtgtccggca cgctggtcag
     5761 ccggacgcgg gcgctgcagc cgtactcggg gatgaccggt tacaacggca ttatcgcccg
     5821 tctgcaacag gctgccagcg acccgatggt ggacggcatt ctgctcgata tggacacacc
     5881 gggcggaatg gtggcgggag catttgactg cgctgacatc atcgcccgtg tgcgtgacat
     5941 aaagccggta tgggcgctgg ccaatgacat gaactgcagc gcaggtcagc tgcttgccag
     6001 tgccgcctcc cggcgtctgg tcacgcagac cgcccggaca ggctccatcg gcgtcatgat
     6061 ggctcacagt aattacggcg ctgcactgga gaaacagggc gtggaaatca cgctgattta
     6121 cagcggcagc cataaggtgg atggcaaccc ctacagccat ctaccggatg atgtccggga
     6181 aacattgcag tcccggatgg atgcaacccg ccggatgttt gcacagaagg tgtcggcata
     6241 taccggcctg tccgtgcagg ctgtgctgga taccgaggct gcagtgtaca gcggtcagga
     6301 ggccattgat gccggactgg ctgatgaact tgttaacagt accgatgcga tcaccgtcat
     6361 gcgtgatgca ctggatgcac gtaaatcccg tctctcagga gggcgaatga ccaaagagac
     6421 tcaatcaaca actgtttcag ccactgcttc gcaggctgac gttactggcg tggtgcaagc
     6481 gacggagggc gagaacgcca gcgcggcgca gccggacgtg aacgcgcaga tcaccgcagc
     6541 ggttgcggca gaaaacagtc gcattatggg gatcctcaac tgtgaggagg ctcacggacg
     6601 cgaagaacag gcacgcgtgc tggccgaaac ccccggtatg accgtggaaa cggcccgccg
     6661 tattctggcc gcagcaccac agagtgcaca gacgcgcagt gacactgcgc tggatcgtct
     6721 gatgcagggg gcaccggcac cgctggctgc aggtaacccg gcatctgatg ccgttaacga
     6781 tttgctgaac acaccagtgt aagggatgtt tatgacgagc aaagaaacct ttacccatta
     6841 ccagccgctg ggcaatagtg acccggctca taccgcaacc gcgcccggcg gattgagtgc
     6901 gaaagcgcct gcaatgaccc cgctgatgct ggacacctcc acccgtaagc tggttgcgtg
     6961 ggatggcacc accgacggtg ctgccgttgg cattcttgcg gttgctgctg accagaccag
     7021 caccacactg acgttctaca agtccggcac gttccgttat gaggatgtgc tctggccgga
     7081 ggctgccagc gacgagacga aaaaacggac cgtgtttgcc ggaacggcaa tcagcatcgt
     7141 ttaactttac ccttcatcac taaaggccgc ctgtgtggct ttttttacgg gattttttta
     7201 tgtcgatgta cacaaccgcc cagctgctgg cggcaaatga gcagaaattt aagtttgatc
     7261 cgctgtttct gcgtctcttt ttccgtgaga gctatccctt cactacggag aaagtctatc
     7321 tctcacaaat tccgggactg gtaaacatgg cgctgtacgt ttcgccgatt gtttccggtg
     7381 aggttatccg ttcccgtggc ggctccacct ctgaatttac gccgggatat gtcaaaccca
     7441 agcatgaggt gaatccgcag atgaccctgc gtcgcctgcc ggatgaagat ccgcagaatc
     7501 tggcggaccc ggcttaccgc cgccgtcgca tcatcatgca gaacatgcgt gacgaagagc
     7561 tggccattgc tcaggtcgaa gagatgcagg cagtttctgc cgtgctcaag ggcaaataca
     7621 ccatgaccgg tgaagccttc gatccggttg aggtggatat gggccgcagt gcggcgaaca
     7681 acatcacaca gtccggcgtc acggagtgga gcaagcgtga caagtccacg tatgacccga
     7741 ccgacgatat cgaagcctac gcgctgaacg ccagcggtgt ggtgaatatc atcgtgtttg
     7801 acccgaaagg ctgggcgctg ttccgttcct tcaaagccgt caaggagaag ctggataccc
     7861 gtcgcggctc tcattccgag ctggagacag cggtaaaaga cctgggcaaa gcggtgtcct
     7921 ataagggaat gtatggcgat gtggccatcg tcgtgtattc cggacagtac gtggaaaacg
     7981 gcgtcaaaaa gaacttcctg ccggacaaca cgatggtgct ggggaacact caggctcgcg
     8041 gtctgcgcac ctatggctgc attcaggatg cggacgcaca gcgcgaaggc attaacgctt
     8101 ctgcccgtta cccgaaaaac tgggtgacca ccggcgatcc ggcgcgtgag ttcaccatga
     8161 ttcagtcagc accgctgatg ctgctggctg atcctgatga gttcgtgtcc gttcaactgg
     8221 cgtaatcatg gcccttcggg gccattttct ctctgtggag gagttcatga cgaaagatga
     8281 actgattgcc cgtcttcagg agctgggtga gcaattgaac cgcgatgtca gtttgacggg
     8341 aacgaaagaa gaactggcgc ttcgtgtggc agagctggaa gaagagcttg atgacacgga
     8401 tgaaactgcc ggtcaggaca cccctctcag ccgggaaaat gtgctgaccg ggcatgaaaa
     8461 tgaggtggta tcagcgcagc cggataccgt gattcaggat acggctgaac tggtcacggt
     8521 cgtggcactg gtgacgctgc atactgatgc acttcacgcc acgcgggata aacctgtggc
     8581 atttgtgctg ccgggaacgg cgtttcgtgt ctctgccggt gtggcagccg aaatgacaga
     8641 gcgcggcctg gccagaatgc aataacggga ggcgctgtgg ctgatttcga taacctgttc
     8701 gatgctgcca ttgcccgcgc cgatgaaacg atacgcgggt acatgggaac gtcagccacc
     8761 atgacatccg gtgagcagtc aggtgcggtg atacgtggtg tttttgatga ccctgaaaat
     8821 atcagctatg ccggacaggg cgtgcgcgtt gaaggctcca gcccgtccct gtttgtccgg
     8881 actgatgagg tgcggcagct gcggcgtgga gacacgctga ccatcggtga ggaaaatttc
     8941 tgggtagatc gggtttcgcc ggatgatggc ggaagttgtc atctctggct tggacggggc
     9001 gtaccgcctg ccgttaaccg tcgccgctga aagggggatg tatggccata aaaggtcttg
     9061 agcaggccgt tgaaaacctc agccgtatca gcaaaacggc ggtgcccggt gccgccgcaa
     9121 tggccattaa ccgcgttgct tcatccgcga tatcgcagtc tgcgtcacag gttgcccgtg
     9181 agacaaaggt acgccggaaa ctggtaaagg aaagggccag gctgaaaagg gccacggtca
     9241 aaaatccgca ggccagaatc aaagttaacc ggggggattt gcccgtaatc aagctgggta
     9301 atgcgcgggt tgtcctgtcc cgccgcaggc gtcataaaaa ggggcagcgt tcatccctga
     9361 aaggtggcgg cagcgtgctt gtggtgggta accgtcgtat tcccggcgcg tttattcagc
     9421 aactgaaaaa cggccggtgg catgttatgc agcgtgtggc cgggaaaaac cgttacccca
     9481 ttgatgtggt gaaaatcccg atggcggtgc cgctgaccac ggcgtttaaa cagaatattg
     9541 aacggatacg gcgtgaacgt cttccgaaag agctgggcta tgcgctgcag catcaactga
     9601 gaatggtaat aaagcgatga aacatactga actccgtgca gccgtactgg atgcactgga
     9661 gaagcatgac accggggcga cgctttttga tggtcgcccc gctgtttttg atgaggaaga
     9721 ttttccggca attgccgttt atctcaccgg cgctgaatac acgggcgaag agctggacag
     9781 cgatacctgg caggcggagc tgcatattga agttttcctg cctgctcagg tgccggattc
     9841 agagctggat tcgtggatgg agtcccggat ttatccggtg atgagcgata tcccggcact
     9901 gtcagatttg atcaccagta tggtggccag cggctatgac taccggcgcg acgatgatgc
     9961 gggcctgtgg agttcagccg atctgactta tgtcattatc tatgaaatgt gaggacgcta
    10021 tgcctgtacc aaatcctaca atgccggtga aaggtgccgg gaccaccctg tgggtttata
    10081 aggggagcgg tgacccttat gcgaacccgc tttcagacgt tgactggtcg cgtctggcaa
    10141 aagttaaaga cctgacgccc ggcgaactga ccgctgagtc ctatgacgac agctatctcg
    10201 atgatgaaga tgcagactgg actgcgaccg ggcaggggca gaaatctgcc ggagatacca
    10261 gcttcacgct ggcgtggatg cccggagagc aggggcagca ggcgctgctg gcgtggttta
    10321 atgaaggtga tacccgtgcc tataaaatcc gcttcccgaa cggcacggtc gatgtgttcc
    10381 gtggctgggt cagcagtatc ggtaaggcgg tgacggcgaa ggaggtgatt acccgcacgg
    10441 tgaaggtcac caatgtggga cgtccgtcga tggcagaaga tcgcagcacg gtgacggcga
    10501 cgaccggcat gacggtgaca cccgccagtg cttccgtagt gaaagggcag agcaccacgc
    10561 tgaccgtggc attccagccg gatggcgcaa ccgacaagag cttccgtgcg gtgtctgcgg
    10621 ataaaacaaa agccaccgtg tcggtcagtg gtatgaccat caccgtgaaa ggtgttgctg
    10681 caggcaaggt caacattccg gtcgtatctg gtaatggtga gtttgctgcg gttgcagaaa
    10741 tcaacgtcac cgccagttaa tccggagagt cagcgatgtt cctgaaaacc gaatcatttg
    10801 aacataacgg tgtgaccgtc acgctttctg aactgtcagc cctgcagcgt attgagcatc
    10861 tcgacctgat gaaacggcag gcagaacagg cggagtcaga cagcaaccgg aagtttactg
    10921 tggaagacgc catcagaacc ggcgcttttg tggttgcgat gtccctgtgg cataaccatc
    10981 cgaagaagac gcagatgccg tccatgaatg aagccgttaa acagattgag caggaagtgc
    11041 ttaccacctg gcccacagag gcaatttctc atgctgaaaa cgtggtgtac cggctgtccg
    11101 gtatgtatga gtttgtggtg aatgatgctc ctgaacaggc agaggacgcc gggcctgcag
    11161 agcctgtttc tgcgggaaag tgttcgacgg tgagctgagt tttgccctga aactggcgcg
    11221 tgagatgggg cgacccgact ggcgcgccat gcttgccggg atgtcatcca cggagtatgc
    11281 cgactggcac cgcttttaca gtacccatta ttttcatgat gttctgctgg atatgcactt
    11341 ttccgggctg acgtacaccg tgctcagcct gtttttcagc gatccggaga tgcatccgct
    11401 ggatttcagt ctgctgaacc ggcgcgaggc tgacgaagag cctgaagatg atgtgctgat
    11461 gcagaaagcg gcagggcttg ccggaggtgt ccgctttggc ccggacggga atgaagttat
    11521 ccccgcttcc ccggatgtgg cggacatgac ggaggatgac gtaatgctga tgacagtatc
    11581 agaagggatc gcaggaggag tccggtatgg ctgaaccggt aggcgatctg gtcgttgatt
    11641 tgagtctgga tgcggccaga tttgacgagc agatggccag agtcaggcgt catttttccg
    11701 gtacggaaag tgatgcgaaa aaaacagcgg cagtcgttga acagtcgctg agccgacagg
    11761 cgctggctgc acagaaagcg gggatttccg tcgggcagta taaagccgcc atgcgtatgc
    11821 tgcctgcaca gttcaccgac gtggccacgc agcttgcagg ggggcagaat ccctggctga
    11881 tcctgctgca acagggtggt caggttaagg actccttcgg cgggatgatc cccatgttca
    11941 gggggcttgc cggcgcgatc accctgccga tggtgggggc cacctcgctg gcggtggcga
    12001 ccggtgcgct ggcgtatgcc tggtatcagg gcaactcaac cctgtccgat ttcaacaaaa
    12061 cgctggtcct ttccggtaat caggcgggac tgacggcaga tcgtatgctg gtcctgtcca
    12121 gagccgggca ggcggcaggg ctgacgttta accagaccag cgagtcactc agcgcactgg
    12181 ttaaggcggg ggtaagcggt gaggctcaga ttgcgtccat cagccagagt gtggcgcgtt
    12241 tctcctatgc atccggcgtg gaggtggaca aggtcgctga agccttcggg aagctgacca
    12301 cagacccgac gtcggggctg acggcgatgg cacgccagtt ccataacgtg acggcggagc
    12361 agattgcgta tgttgctcag ttgcagcgtt ccggcgatga agccggggca ttgcaggcgg
    12421 cgaacgaggc cgcaacgaaa gggtttgatg accagacccg ccgcctgaaa gagaacatgg
    12481 gcacgctgga gacctgggca gacaggactg cacgtgcatt caaatccatg tgggatgcgg
    12541 tgctggatat tggtcgtcct gataccgcgc aggagatgct gattaaggca gaggctgcgt
    12601 ttaagaaagc agacgacatc tggaatctgc gcaaggatga ttatttcgtt aacgatgaag
    12661 cacgggcgcg ttactgggat gatcgtgaaa aaaaacgcct ggagcgggat gcagcccaaa
    12721 agagggtaga tcaacagcgt caacaggaca aaaatgcgca gcagcagagc gataccgaag
    12781 cgtcacggct gaaatatacc gaagaggcgc agaaggctta cgaacggctg cagacgccgc
    12841 tggagaaata taccgcccgt caggaagaac tgaacaaggc actgaaagac ggaaaaatcc
    12901 tgcaggcaga ttacaacacg ctgatggcgg cggcgaaaaa ggattatgaa gcgacgctga
    12961 aaaagccgaa acagtccggc gtgaaggtgt atgcgggcga tcgtcaggaa gacagtgctc
    13021 atgcggccct gctgacgctt caggcagaac tccggacgct ggagaagcat gccggagcga
    13081 atgagaaaat cagccagcag cgccgggatt tgtggaaggc agaaagtcag ttcgcggtac
    13141 tggaggaggc ggcacaacgt cgccagctgt ccgcacagga gaaatccctg ctggcgcata
    13201 aagacgagac gctggagtac aaacgccagc tggctgcact tggtgacaag gttacgtatc
    13261 aggagcacct gaatgcgctg gcgcagcagg cggataaatt cgcacagcag caacgggcaa
    13321 aacgggcagc cattgatgcg aaaaaccggg ggctgactga ccggcaggca gcgcgggaag
    13381 ccacggaaca gcgcctgaag gaacagtatg gcgataatcc tctggcgctg aataacgtca
    13441 tgtcagagca gaaaaagacc tgggcggctg aagaccagct tcgcgggagc tggatggcag
    13501 gcctgaagtc cggctggagt gagtgggaag agagcgccac ggacagtatg tcacaggtta
    13561 aaagtgcagc cacgcagacc tttgatggta ttgcacagaa tatggcggcg atgctgaccg
    13621 gcagtgaaca gaactggcgc agcttcaccc gctccgtgct gtccatgatg acagaaattc
    13681 tgcttaagca ggcaatggtg gggattgtcg ggagtatcgg cagcgccatt ggcggggctg
    13741 ttggtggcgg cgcatccgcg tcaggtggta cagccattca ggccgctgcg gtgaaattcc
    13801 attttgcaac cgggggattt acgggaaccg gcggcaaata tgagccagcg gggattgttc
    13861 accgtggtga atttgtcttc acgaaggagg caaccagccg gattggcgtg gggaatctct
    13921 accggctgat gcgcggctat gcggaaggtg gttatgtggg cggtgccgga agtccggcgc
    13981 agatgcggcg ggctgaaggc attaatttta atcagaacaa tcacgtggtg attcagaacg
    14041 acggtacgaa tggtctgcca ggtccacaga tgatgaaggc cgtgtatgac atggcccgca
    14101 agggtgcccg tgatgaaatt cagacacaga tgcgtgatgg tggcctgttc tccggaggtg
    14161 gacgatgaaa accttccgct ggaaagtgaa acccggtatg gatgtggctt cggccccttc
    14221 tgtaagaaag gtgcgctttg gtgatggcta ttcccagcga gcgcctgccg ggctgaatgc
    14281 caacctgaaa acgtacagcg tgacgctttc tgtcccccgt gaggaggcca tggtactgga
    14341 gtcgtttctg gaagagcacg ggggctggaa agcctttctg tggacgccgc cttatgagtg
    14401 gcggcagata aaggtgacct gcgcaaaatg gtcgtcgcgg gtcagtatgc tgcgtgttga
    14461 gttcagcgca gagtttgaac aggtggtgaa ctgatgcagg atatccggca ggaaacactg
    14521 aatgaatgca cccgtgcgga gcagtcggcc agcgtggtgc tctgggaaat cgatctgaca
    14581 gaggtcggtg gagaacgtta ttttttctgt aatgagcaga acgaaaaagg tgagccggtc
    14641 acctggcagg ggcgacagta tcagccgtat cccattcagg ggagtggttt tgaactgaat
    14701 ggcaaaggca ccagtacgcg ccccacgctg atggtttcta acctgtacgg tatggtcacc
    14761 gggatggcgg aagatctaca gagtctggtc ggcggaacgg tggtccggcg taaggtttac
    14821 gcccgttttc tggatgcggt gaacttcgtc aacggaaaca gtgacgccga tccggagcag
    14881 gaggtgatca gccgctggcg cattgagcag tgcagcgaac tgagcgcggt gagtgcctct
    14941 tttgtactgt ccacgccgac ggaaacagat ggcgctgttt ttccgggacg tatcatgctg
    15001 gccaacacct gcacctggac ctatcgcggc gatgagtgcg gttatcacgg tccggcggtc
    15061 gcggatgaat atgaccagcc aacgtccgac atcacgaagg ataaatgcag caaatgcctg
    15121 agtggctgta agtttcgcaa taacgtcggc aactttggcg gcttcctttc cattaacaaa
    15181 ctttcgcagt aaatcccatg acagagacag aatcagcgat tctggcgcac gcccggcgat
    15241 gtgctccagc ggagtcgtgc ggcttcgtgg taagaacgcc ggagggggaa agatatttcc
    15301 cctgcgtgaa tatctccggt gagccggagg cgtatttccg tatgtcgccg gaagactggc
    15361 tgcaggcaga gatgcagggt gagattgtgg cactggtcca cagccacccc ggtggtctgc
    15421 cctggctgag tgaggccgac cggcggctgc aggtgcagag tgatttgccg tggtggctgg
    15481 tctgccgggg ggcgattcac aagttccgct gtgtgccaca tcttaccggg cggcgctttg
    15541 agcacggggt gacggactgt tacacgctgt tccgggatgc ttaccatctg gcggggattg
    15601 agatgccgga ttttcatcgt gaggatgact ggtggcgtca cggtcagaat ctctatctgg
    15661 ataatctgga ggccacaggg ctgtatcagg tgccgttgtc agcggcgcag ccgggcgatg
    15721 tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac tgtggtgacg
    15781 gcgagctgct gcaccatatt cctgaacaac tgagcaaacg agagaggtat accgacaaat
    15841 ggcagcgacg cacacactcc ctctggcgtc accgggcatg gcacgcatct gcctttacgg
    15901 ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg aaaacgggtg ccgaagccat
    15961 ccgggcgatg gccacacagc tccctgcgtt tcgtcagaag ctgagcgacg gctggtatca
    16021 ggtgcgtatt gccgggcagg atgttagcac gtccggatta acggcacagt tacatgaggc
    16081 tctgcctgac ggcgctgtga ttcatattgt tcccagagtc gccggggcca agtcaggtgg
    16141 tgtattccag attgtcctgg gagcagccgc cattgccgga tcattcttta ccgccggagc
    16201 cacccttgca gcatgggggg ctgccattgg tgccggtggt atgaccggca ttctgttttc
    16261 tctcggtgcc agtatggtgc tcggcggtgt ggctcagatg ctggcaccga aagccagaac
    16321 tccccgcaca cagacaacgg ataacggtaa gcagaacacc tatttctcct cactggataa
    16381 catggttgcc cagggcaatg ttatgcctgt tctgtatggt gaaatgcgcg tggggtcacg
    16441 tgtggtatct caggagatca gcacggcaga cgaaggggac ggtggtcagg ttgtggtgat
    16501 tggtcgctga tgcaaaatgt tttatgtgaa atcgcctccg ggcggtttta tcgtttatgg
    16561 agcatgacga atgggtaaag gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa
    16621 cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc agcgaagggc cggttgaagg
    16681 tccggtggat ggattaaaaa gcgtgctgct gaacagtacg ccggtgctgg acagtgaggg
    16741 gaataccaac atctccggcg tcacggtggt gttccgggca ggtgagcagg agcagtcacc
    16801 gccggaggga tttgaatcct ccggctccga gacggtgctg ggtacggaag tgaaatatga
    16861 cacgccgatc acccggacca tcacgtcggc aaacattgac cgactgcgtt ttaccttcgg
    16921 tgtacaggca ctggtggaaa ccacctcaaa gggagacagg aatccgtctg aagttcgcct
    16981 gctggttcag atacagcgta acggtggctg ggtgacggaa aaagacatca ccattaaggg
    17041 caaaaccacc tcgcagtatc tggcctcggt ggtggtggat aacctgccgc cgcgcccgtt
    17101 taatatccgg atgcgcagaa tgacgccgga cagcaccaca gaccagctgc agaacaaaac
    17161 gctctggtcg tcatacaccg aaatcatcga tgtgaaacag tgctacccga acacggcact
    17221 ggtcggcgta caggtggatt cggagcagtt cggcagccag caggtgagcc gtaattatca
    17281 tcttcgcggg cgcattctgc aggtgccgtc gaactataat ccgcagacgc ggcaatacag
    17341 cggtatctgg gacggaacgt taaaaccggc atacagcaac aacatggcct ggtgtctgtg
    17401 ggatatgctg acccatccgc gctatggcat ggggaaacgt cttggtgcgg cagatgtgga
    17461 taaatgggcg ctgtatgtca tcggccagta ctgtgaccag tcggtgccgg acggctttgg
    17521 cagcacggag ccgcgcatca cctgtaatgc ctggctgacc acacagcgca aggcgtggga
    17581 tgtgctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct
    17641 gacgttcgtg caggaccgac agtcggataa ggtgtgggcc tataaccgca gtaatgtggt
    17701 gatgccggat gatggcgcgc cgttccgcta cagtttcagc gccctgaaag accgccataa
    17761 tgccgttgag gtgaactgga ttgacccgga taacggctgg gagacggcga cagagcttgt
    17821 ggaggatacg caggccattg tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg
    17881 ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa cggaactgct
    17941 ggagacgcag accgtggatt tcagcgtggg tgctgaaggg cttcgtcatg taccgggcga
    18001 tgtcattgaa atctgcgatg atgactatgc cggtatccgc accggcgggc gcgtgctggc
    18061 ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg
    18121 caccacgctg ataagcctgg ttgacgggca ggggaatccg gtcagcgtgg aggtccagtc
    18181 cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg ctgaatacag
    18241 cgtgtggggg ctgaagctgc cgacgctgca ccagcgattg ttccgctgcg tgagtatccg
    18301 tgagaacgac gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaagc
    18361 catcgtggat aacggggcgc actttgacgg cgaccagagc ggcacggtga atggtgtcac
    18421 gccgccagcg gtgcagcacc tgactgccga agtcaccgca gacagcgggg aatatcaggt
    18481 gctggcgcgc tgggacacgc cgaaggtggt gaagggcgtg agcttcctgc tccgtctgac
    18541 cgtaacagcg gatgacggca gtgagcggct ggtcagcacg gcccggacga cggaaaccac
    18601 ttaccgcttc acacaactgg cgctggggaa ctacaggctg acagtccggg cagtaaatgc
    18661 gtgggggcag cagggcgatc cggcgtcggt atcgttccgg attgccgcac cggcagcgct
    18721 gtcgcggatt gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgcggt
    18781 ttatgatccg acggtacagt ttgagttctg gttctcggaa aagcggattg cggatatcag
    18841 gcaggttgaa accacagcac gctatcttgg cacggcgctg tactggatag ccgccagtat
    18901 caatatcaaa ccgggccatg attattactt ttatatccgc agtgtgaaca ccgttggcaa
    18961 atcggcattc gtggaggctg ttggtcagcc gagtgatgat gcatccggtt atctggattt
    19021 tttcaaaggc gagataggga aaacccatct ggctcaggag ctgtggacgc agattgataa
    19081 cggtcagctt gcgcctgacc tggctgaaat caggacgtcc attacggatg tcagcaatga
    19141 aatcacgcag accgtcaata agaaactgga agaccagagt gcggcaattc agcagataca
    19201 gaaggttcag gttgatacaa ataataacct gaacagcatg tgggctgtga agctgcagca
    19261 gatgcaggac ggacgccttt atatcgcggg tattggtgcc ggtattgaga acacccctga
    19321 cggcatgcag agtcaggtgc tgctggcggc ggacaggatt gcgatggtta atcctgcgaa
    19381 tggcaacaca aaaccgatgt ttgttggtca gggcgatcag atattcatga acgacgtgtt
    19441 cctgaaacgc ctgacggccc ccaccattac cagcggtgga aatccatcgg cattttccct
    19501 gactccggac ggaaagctga ccgctaaaaa tgcggatatc agtggcagtg tgaatgcgaa
    19561 cgccgggacg ctcaacaatg tcacggtaaa tgaaaactgt acgattaagg gcatgctgga
    19621 ggcgactcag gtcagaggtg acttcgttaa agctgtatcc aaatcatttc cgaaacaggc
    19681 tggtacgtgg ggtaacacgg aaacaccaaa cgggacggtt acagtcacca taagcgatga
    19741 tcataacttt gaccgccaga ttattattcc gcccattatt tttaacggta tagcgtatga
    19801 cgatccgggg agcggaaata acccaggagg cacgcgatac acgggttatg gttttgaagt
    19861 tcgcaaaaac ggcgtattaa tcgcatccag agaaactaaa ggtgccattc ccggtagtta
    19921 cagtgcagtt attgatatgc ctagtggtgg tggtagcgtc actctggagt ttaagatttt
    19981 ccagaaaggc aatcaggggg caggcaatat caccgactgt acggtgattg tgaccaaaaa
    20041 agcggcttcc ggcatcagta ttcgttgaaa tatttataac cccaataaat ggcgtcagga
    20101 atgacgcctt ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca
    20161 ttttgtccgc agccatttgc ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc
    20221 agtccacgct gagcgcgggg tatcttcatg cccggaccaa cgttcccggc agtgatgatc
    20281 tgaacgggat taacgtgaaa taccgttatg agtttacgga tacgctgggg ctggtgacgt
    20341 cattcagcta tgcaggagac aagaatcgcc agctgacccg ttacagcgat acccgctggc
    20401 atgaagattc cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga
    20461 atgaatggtt cagcgcgtat gcgatggcgg gtgtggctta cagccgtgtg tcgacttttt
    20521 ccggggatta tatccgcgta actgacaata aggggaaaac gcacgatgtg ctgaccggaa
    20581 gtgatgacgg tcgccacagc aacacgtctc tggcgcactg acggtttcat cgtgggtgtc
    20641 ggttataaat tctgattagc caggtaacac agtgttatga cagcccgccg gttcaggcgg
    20701 gcttttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag acggcacagg
    20761 aaaaccggta cagaactgca caatccagct gaaagcaaaa cgtaacagca ccacggtggt
    20821 ggtgaacacg ctggcctcag aaaatccgga tgaagccggg cgttacagca tggacgttga
    20881 gtacggtcag tacagcgtta ttctgttggt ggaaggattc ccgccgtcac atgccgggac
    20941 catcaccgtg tatgaagatt cccgacccgg tacgctgaat gattttctcg gtgccatgac
    21001 ggaggatgat gcccgtccgg aggcactgcg ccgttttgag ctgatggtgg aagaggtggc
    21061 gcgtaacgcg tccgcggtgg cacagaacac ggcagccgcg aagaaatcag ccagcgatgc
    21121 cagcacatca gcccgtgagg cggcaaccca tgcgactgat gctgcagatt cagcacgtgc
    21181 agccagcacg tcagccggac aggccgcttc gtcggctcag tcagcgtctt ccagcgcagg
    21241 aacggcatca acaaaggcca ctgaagcctc aaaaagtgct gccgctgcag agtcctcaaa
    21301 aagcgcggcg gctaccagtg ccggtgcggc gaaaacgtta gaaacgaatg cggcagcgtc
    21361 acaacaatca gcagccactt ctgcatccac cgcgaccacg aaagcgtcag aagctgccac
    21421 ctcagcccgg gatgcgtcgg cttcaaaaga ggcggcaaaa tcatcagaaa cgaacgcatc
    21481 atcaagcgcc agtagcgcag cttcctcggc gacggcggct gcaaattctg cgaaggcggc
    21541 caaaacgtcc gagacgaacg ccaggtcttc tgaaacggca gcgggacaga gtgcctcagc
    21601 tgcggcaggc tcaaaaacag cggctgcgtc gtctgccagc gccgcgtcaa caagtgccgg
    21661 gcaggcctca gccagtgcca ccgccgccgg aaaatcggca gaaagcgccg catcatccgc
    21721 ttcaacagcc acaacgaagg ctggcgaagc cactgagcag gccacggcag cagcgaggtc
    21781 agcttccgca gcgaagacat ccgaaacgaa cgcgaaagcg tcggaaaccc gtgcagaatc
    21841 ctcaaaaacg gctgccgcat cgtccgccag ttcggcggcg tcatcggcat catcggcgtc
    21901 tgcttcaaaa gatgaggcga ccagacaggc gtcagcagcg aagggcagcg ccacgacggc
    21961 atccacgaag gcgacagagg ctgctggtag tgcgacggca gcagctcaga gcaaaagtac
    22021 ggcggaatct gcagcaacgc gcgctgagac agcggcaaaa cgggcagagg atattgcatc
    22081 cgccgtggcg cttgaggatg cgagcacgac gaaaaagggg atagtacagc tcagtagtgc
    22141 gactaacagc acttccgagt cactggcggc aacaccaaaa gccgttaagg ccgcgtatga
    22201 cctggctaac gggaaataca ccgcacagga tgcaacgaca gcacagaaag ggataatcca
    22261 gctaagcagc gcgaccaaca gcacgtctga aacgctggcg gcaacgccaa aggcagtaaa
    22321 agcagccaat gacaatgctg agaaacgtct gcagaaagat cagaacggtg cggatatccc
    22381 tggcaaagac acctttacga aaaatattgg tgcctgccgt gccttcggtg ggtcagtaag
    22441 cacaacaaca ggaaactgga cgactgcaca gtttatcgag tggctggatt ctcagggagc
    22501 atttaaccat ccatactgga tgtgcaaggg ttcctggtct tatggcaata ataaaatcat
    22561 tactgatact ggctgcggta atattcatct cgccggagct gtcattgaag taatggggat
    22621 aaagtcagcg atgacgatcc gcattaccac tccgaccaca tccagcggtg gcggaacaac
    22681 caacgcccag tttacctata ttaatcacgg aacagattat tcacctggct ggcgaaggga
    22741 ctataactcc agaaataagc caacggcatc agagatcggg gcgctaccgt caggtggaac
    22801 agcagtatca tcagttaatc tggcttcaaa aggtcgggta gccgcgctga cagacaatac
    22861 gcagggggca gcaggtcttg agttatacga ggtgtataac aacggatatc caacagcgta
    22921 tggaaatatc attcacctga aagggatgac agccgttggc gaaggtgagt tactcatcgg
    22981 ctggagtggt acaagcggtg ctcatgctcc ggcatttatt cgttcacgac gggatacgac
    23041 cgacgcaaac tggtcgccgt gggcgcagct ttacacctcg gctcatcctc ctgcagagtt
    23101 ttatccagtc ggtgcaccaa tcccgtggcc atcagatacc gttccgtctg gttatgccct
    23161 gatgcagggg cagacttttg acaaatctgc ttacccgaaa cttgcagccg cttatccgtc
    23221 aggcgtgatc cctgatatgc gtggctggac gattaagggc aagcccgcca gtggtcgtgc
    23281 cgtattgtct caggaacagg atggcattaa atcgcacacc cacagcgcca gcgcatccag
    23341 tacggatttg gggacgaaaa ccacatcgtc gtttgattac ggcactaaat ccacgaataa
    23401 caccggggcg catacgcaca gtctgagcgg ctctacaagt tcagctgggg cccaccaaca
    23461 tagtcagact gggcccagaa cgaatagcgg gagtcaacct actggaatgt ttccggcagg
    23521 gagtactcaa gttagcggga ctaatcaggt tggtatcagt ggctcattaa ccagtggcac
    23581 ctcccaatgg gtaggtaaat catcttcaga aggaaaccat acacactcac tgtccggcac
    23641 agcagcatct gcgggtgcac acgcgcatac tgtcggtatt ggtgctcata cgcactctgt
    23701 tgcgattggt tcacatggac acaccatcac cgttaacgct gctggtaacg cggaaaacac
    23761 cgtcaaaaac atcgcattta actatattgt gaggctcgca taatggcatt cagaatgagt
    23821 gaacaagcac ggaccataaa aatttataac ctgctggccg gaactaatga atttattggt
    23881 gaaggtgacg catatattcc gcctcataca ggtctgcctg caaacagtac cgatattgca
    23941 ccgccagata ttccggctgg tttcgtggct gttttcaaca gtgatgagtc atcgtggcat
    24001 ctcgttgaag atcatcgggg taaaacggtt tatgacgtgg cttccggcga cgcgttattt
    24061 atttctgaac tcggctcatt accggaaaat gtcacctggt tatccccgga aggggagttt
    24121 cagaagtgga acggcacagc ctgggtgaag gatacggaag cagaaaaact gttccggatc
    24181 cgggaggcgg aagaaacaaa aaacagcctg atgcaggtag ccagtgagca tatagcgccg
    24241 cttcaggatg ctgtggatct ggagatcgca acggagaaag aaaccttgtt gctggaagct
    24301 tggaaaaagt atcgggtgtt gctgaaccgt gttgatacat caactgcacc ggatattgaa
    24361 tggccagtag cacctatagg gtaaattcgt aatgattacc taaatacgtt attcttttgt
    24421 taaaaaagtg attttgctat aggtagggag tatgttcttg gattaagaat aaaaaatcgc
    24481 catgaaagag cttcatggcg agtacataaa cgtacaaaat gtgatgaaag tagagtg
//
LOCUS       DAZPAC010000046        24309 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZPAC010000046 DAZPAC010000000
VERSION     DAZPAC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.046"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(1..413)
                     /locus_tag="ACHUPM_001533"
                     /pseudo
     CDS             complement(1..413)
                     /locus_tag="ACHUPM_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(519..3080)
                     /gene="mutS"
                     /locus_tag="ACHUPM_001534"
     CDS             complement(519..3080)
                     /gene="mutS"
                     /locus_tag="ACHUPM_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR0869355.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            3156..3290
                     /locus_tag="ACHUPM_001535"
     CDS             3156..3290
                     /locus_tag="ACHUPM_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869356.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            3367..3720
                     /locus_tag="ACHUPM_001536"
     CDS             3367..3720
                     /locus_tag="ACHUPM_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR0869357.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(3757..5834)
                     /gene="flhA"
                     /locus_tag="ACHUPM_001537"
                     /pseudo
     CDS             complement(3757..5834)
                     /gene="flhA"
                     /locus_tag="ACHUPM_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(5908..6918)
                     /gene="hypE"
                     /locus_tag="ACHUPM_001538"
     CDS             complement(5908..6918)
                     /gene="hypE"
                     /locus_tag="ACHUPM_001538"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR0869358.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(6915..8036)
                     /gene="hypD"
                     /locus_tag="ACHUPM_001539"
     CDS             complement(6915..8036)
                     /gene="hypD"
                     /locus_tag="ACHUPM_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR0869359.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(8036..8308)
                     /gene="hypC"
                     /locus_tag="ACHUPM_001540"
     CDS             complement(8036..8308)
                     /gene="hypC"
                     /locus_tag="ACHUPM_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR0869360.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(8299..9171)
                     /gene="hypB"
                     /locus_tag="ACHUPM_001541"
     CDS             complement(8299..9171)
                     /gene="hypB"
                     /locus_tag="ACHUPM_001541"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR0869361.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(9175..9525)
                     /gene="hypA"
                     /locus_tag="ACHUPM_001542"
     CDS             complement(9175..9525)
                     /gene="hypA"
                     /locus_tag="ACHUPM_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR0869362.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            9737..10198
                     /gene="hycA"
                     /locus_tag="ACHUPM_001543"
     CDS             9737..10198
                     /gene="hycA"
                     /locus_tag="ACHUPM_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR0869363.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            10323..10934
                     /gene="hycB"
                     /locus_tag="ACHUPM_001544"
     CDS             10323..10934
                     /gene="hycB"
                     /locus_tag="ACHUPM_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR0869364.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            10931..12757
                     /gene="hycC"
                     /locus_tag="ACHUPM_001545"
     CDS             10931..12757
                     /gene="hycC"
                     /locus_tag="ACHUPM_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFR0869365.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCDYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            12760..13683
                     /gene="hycD"
                     /locus_tag="ACHUPM_001546"
     CDS             12760..13683
                     /gene="hycD"
                     /locus_tag="ACHUPM_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR0869366.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            13701..15398
                     /gene="hycE"
                     /locus_tag="ACHUPM_001547"
     CDS             13701..15398
                     /gene="hycE"
                     /locus_tag="ACHUPM_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR0869367.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            15408..15950
                     /gene="hycF"
                     /locus_tag="ACHUPM_001548"
     CDS             15408..15950
                     /gene="hycF"
                     /locus_tag="ACHUPM_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFR0869368.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            15950..16717
                     /gene="hycG"
                     /locus_tag="ACHUPM_001549"
     CDS             15950..16717
                     /gene="hycG"
                     /locus_tag="ACHUPM_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFR0869369.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            16714..17124
                     /gene="hycH"
                     /locus_tag="ACHUPM_001550"
     CDS             16714..17124
                     /gene="hycH"
                     /locus_tag="ACHUPM_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFR0869370.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            17117..17587
                     /gene="hycI"
                     /locus_tag="ACHUPM_001551"
     CDS             17117..17587
                     /gene="hycI"
                     /locus_tag="ACHUPM_001551"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFR0869371.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            17612..18385
                     /locus_tag="ACHUPM_001552"
     CDS             17612..18385
                     /locus_tag="ACHUPM_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869372.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            18547..18858
                     /locus_tag="ACHUPM_001553"
     CDS             18547..18858
                     /locus_tag="ACHUPM_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFR0869373.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            18855..19274
                     /locus_tag="ACHUPM_001554"
     CDS             18855..19274
                     /locus_tag="ACHUPM_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0869374.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(19388..20812)
                     /gene="ascB"
                     /locus_tag="ACHUPM_001555"
     CDS             complement(19388..20812)
                     /gene="ascB"
                     /locus_tag="ACHUPM_001555"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFR0869375.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(20821..22278)
                     /gene="ascF"
                     /locus_tag="ACHUPM_001556"
     CDS             complement(20821..22278)
                     /gene="ascF"
                     /locus_tag="ACHUPM_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFR0869376.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22538..23548
                     /gene="ascG"
                     /locus_tag="ACHUPM_001557"
     CDS             22538..23548
                     /gene="ascG"
                     /locus_tag="ACHUPM_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR0869377.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            23696..24223
                     /gene="hydN"
                     /locus_tag="ACHUPM_001558"
     CDS             23696..24223
                     /gene="hydN"
                     /locus_tag="ACHUPM_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR0869378.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
BASE COUNT         5493 a         6425 c         6606 g         5785 t
ORIGIN      
        1 atggcatatt ttatgttgtc attaatgatt tcaataatat gtggaaggtg atggaatttc
       61 agcaacagat ctattgcctc ttgttgctct gaatcattta aatcgaaaaa ccagtcacca
      121 ccgctggcaa gccacatatt tccatcgcca gtttcgaatg catccaacgc catcgcttcg
      181 tggttgcctt taaccgacgt aaaccagggt tggtttagca ggcgcagcac gttaagactc
      241 ttcggcccac gatcaatatt atcgcctgtg gaaataagta agtcggtttc agggtaaaaa
      301 gagagttgat gttaacggga ttgtaataac tgatattcac catgaatatc accaacgacc
      361 catatatggc gatagtgatg ggcattgatt ttttgatagc gtgtagatgg catggtttta
      421 ccctgtaaaa taagctttcc tattatacag ggtattttta tttgattcgt cagttgtcgt
      481 taatattccc gatagcaaaa gactatcggg aattgttatt acaccagact cttcaagcga
      541 taaatccact ccagcgcctg acgcggagtc agtgaatccg ggtcgagatt ttccagtgct
      601 tcgaccgcag gcgaagtttc ttctggcact gacagcaaag acatctgcgt accatccact
      661 tgcgtcgcgg cggcgttcgg cgaaatgctt tccagctcac gcagtttttg ccgtgcgcgc
      721 ttaataacct ctttcggtac gcccgccaga gcagcaaccg ccagaccgta gcttttgctc
      781 gccgcgccgt cctgcacgct gtgcataaag gcgatagtgt cgccgtgctc cagcgcatcg
      841 agatgtacgt tggcgacgcc ttccattttc tccggtagct gggtcagctc gaaatagtgg
      901 gtggcaaaca gcgtcaatgc cttaatctta ttcgccagat tttctgcgca cgcccacgcc
      961 agcgacagac catcgtaggt ggacgttcca cgcccgatct catccattaa caccagactg
     1021 tattcggtgg cgttatgtaa aatattggcg gtttcagtca tctccaccat aaaggttgag
     1081 cgcccggaag ccaggtcatc tgccgcgcct acgcgggtaa agatgcgatc gataggtcca
     1141 atctcgactt tttgtgccgg tacatagctg ccgatgtagg ccatcagcgc aatcagtgcg
     1201 gtctggcgca tataggtact tttaccgccc atgttcggac cggtgatgat caacatacgg
     1261 cgctgcggcg acagattcag cgggttagcg ataaatggct cattcagtac ttgttcaact
     1321 accggatggc gaccttcggt aatgcgaatg cccggtttat caatgaaggt cgggcaggtg
     1381 tagttcaggg tataggcccg ttccgccagg ttcaccagca cgtcgagttc cgccagcgcg
     1441 ctcgcgctct gttgcaacgc ttccagatgc ggcaacagca ggtcgaacaa ctcttcataa
     1501 agctgttttt ccagtgccag tgctttgcct tttgaggtga gaactttatc ttcgtactct
     1561 tttagctctg gaatgatgta gcgctcggcg tttttcagcg tctggcgacg catgtagttg
     1621 atgggtgcca gatggctttg cccacggctg atttgaatgt agtagccgtg caccgcatta
     1681 aagccaactt tcagcgtgtc caggccggta cgttcacgct cgcggacttc cagacgctcc
     1741 agataatcgg tcgcgccgtc agccagcgcg cgccactcat ccagctcttc gttatagccc
     1801 gatgcgataa caccaccgtc gcgtaccagc accggcggtg tgtcgatgat tgctcgctcc
     1861 agcagatcgc gcagctcggc aaactcgccc atcttctcac gtagcgcctg taccggtgca
     1921 ctatcgacag tttctaactg cgcacgcagc tccggcagtt gctggaaagc gtggcgcata
     1981 cgggccagat cgcgtgggcg agcagttcgt aaagccagac gtgccagaat acgttccagg
     2041 tcgccgacct gacgcagtac cggctgcaac tcggcggtga aatcctgcaa tgcgccaata
     2101 gtttgctggc gctcaagcaa cacgcgggta tcgcgcactg gcatatgcag ccagcgtttc
     2161 agcatacgac tacccatcgg cgtgacggtg cagtcgagca cagaagccag cgtattttcc
     2221 gcaccgcccg ccaggttctg agtaatttcc aggttacgac gtgtcgcggc atccataatg
     2281 atgctgtcct gctcacgttc catggtgata gaacgaatat gcggcagggt cgtgcgttgg
     2341 gtatctttcg catactgcaa cagacaaccg gcagcacaaa gtccgcgcgg cgcgttctcg
     2401 acgccaaaac cgaccagatc gcgggtgcca aattgcagat tcaactgctg gcgcgcggtg
     2461 tcgatttcaa actcccacag cgggcgacgg cgcaggccgc gacggccttc aatcagcgac
     2521 atctcggcga aatcttctgc atacagcagt tccgccggat tagtgcgttg cagctctgcc
     2581 gccatcgttt cgcggtcggc cggttcgctc aggcgaaaac gcccggagct gatatccagc
     2641 gtcgcgtagc cgaaaccttt gctgtcctgc cagatagccg ccagcaggtt gtcctgacgc
     2701 tcctgtaaca gggcttcatc gctgatggtg cctggcgtaa cgatacgcac aactttgcgc
     2761 tcaaccggac ctttgctggt cgccggatcg ccaatttgtt cgcagatggc aacggactct
     2821 ccctgattca ccagtttggc gagatagttt tccaccgcat ggtagggaat ccccgccatc
     2881 gggatcggct ctcccgccga agcaccgcgt ttggtcagtg aaatatccag cagttgcgac
     2941 gcgcgttttg cgtcgtcata aaacagttca taaaaatcac ccatccggta aaacagcagg
     3001 atctcgggat gctgggcttt cagcctgaga tactgctgca tcatgggcgt atgggcgtcg
     3061 aaattttcta ttgcactcat ggggttatgt ccggttccct gatattaaat ggggtgtgat
     3121 ggtttttatt ttcgttgtaa tcacataagg cgcacatgat accggagtta atcatttcag
     3181 gggaagaagc gaacggcgag ttcttgatgc tggttccggg aaagatgctg ttttgcagct
     3241 tttgcgtgat gcttatgaag caagtacgca aaattgcgtc ggttgaataa acctcctcct
     3301 tcagagccat agtccccttt caaattaagt tgtaatataa atacacctta agagtatgga
     3361 ggcagcatgt ccggcaagcg tatctctcgt gaaaaactga cgattaaaaa aatgatcgat
     3421 ctttatcaag cgaaatgccc gcaggcgtca gcggagccgg agcattacga ggcgttgttt
     3481 gtttacgcgc aaaagcggct ggataaatgt gtgttcggcg aagagaaacc agcctgtaag
     3541 cagtgtccgg tgcactgtta ccagcctgca aagcgtgaag agatgaaaca gattatgcgt
     3601 tgggccgggc cgagaatgct gtggcgtcat ccgatcttaa ccgtgcgtca tctcatcgat
     3661 gacaagcgtc cggtaccaga actgccggaa aaatatcgtc cgaaaaaacc tcgtgaatag
     3721 tatgccggac aggcagatct gtccggcaat ttgcagttaa atcaatgccg atttatcaat
     3781 tcccagccgc ttcatccgtg acagcagggt cgtgcgtttc agccccagac gttgcgcagc
     3841 gcctttaggc ccggcaacca cgccgttggt ttctttcagc acgcgcacaa tcaactgata
     3901 ttcatcttcg ccctcctggg cgacaaccgt tgcggcaggc ggcgtttcag gttccggtaa
     3961 aacaatatct ggcaatgaca gctgcagcac gttaccgcgt gttagcaata ccgcgcgctc
     4021 aatgacgttt tccagttcgc gtacgttacc cggccactcc atgttgctca aggtgcgcag
     4081 cgtctcggca ggaatgctgt cgatattgcg ccccagacga cgggcaattt tgaaggtaaa
     4141 ggctttcgcc agcagcggaa tatcttccgg acgctcgcgt agtggcggca ggtgaatcgg
     4201 gaatacgttc aggcggtaat agagatcgct acggaactct cggtcggcga ccattttttt
     4261 cagatcgcgg ttagtcgcgg caattaaacg tacgtccgtc tgaatgattt tgttgctgcc
     4321 gagacgttca aactcctgtt cctgcaatac acgcagcaac ttcggctgta actccagtgg
     4381 catatcgccc acttcgtcga ggaacagaga gcttttatcc gccagttcaa aacgaccaat
     4441 gcgctgggcg ctggcaccgg taaaagcccc acgctcatga ccaaacagat cgctttccag
     4501 caatccggca ggcatcgccg cgcagttcat tttgaccatg cggcgattat tacgcccact
     4561 gagattatgg atcgcgcggg caatcagctc tttacccgtg ccagtttcac cgaggatcag
     4621 cacggtactg tcactttgcg ccaccatttc aacttgttta agcacgctgt acatggcttc
     4681 gctgcggcca ataatctcgc caaattcact atcaacattg ttgagctgct cggtcagggc
     4741 gaggttttca tcaaccagcc gttctttcag acgatggatt tcctgatagg cgagggcgtt
     4801 atcgacagcg attgccacac gttcggcaat ctggcgcagc aaattcagat tggtagtggt
     4861 aaacactttc tcttcgcatt gcgccagttt cagcacgccc agcatggtgt cgccagacat
     4921 cagcggtaac aggcacaagg tttgaatctg gttgccctag gtgtcgaaca acatgcgttc
     4981 atagggggct aaatcgtccc gttcgtggag attgatcagc agcatctctt tacttttgaa
     5041 cacgcgttcg gtgagggttc cggcttcatc gacttcgctc tgttcgtggg cgggatgttg
     5101 tttatcaaga tagtgagtgg agtagatgtt gagtttgttt ttacggtggc tgcgtaagac
     5161 gatactgata tcgtcaatgt cgaaatagta atggatttct ttggcgactt cgctgaccag
     5221 ttcgtccata tccaggcggg aaagcaccgc gttggtaatg gcaaccagga tgcggaagtt
     5281 atcgcgctcc cggcataaca actcatagtc gacattgttg acgacgcggc tctggatttg
     5341 ttcggtgacg acagaaacga tctgcgtaaa tgtttgcaga cgattgaact ctttttcgct
     5401 ccagggacga tcgtcataac gaataaattc acagccacca aaaatatgcc cttccgccgc
     5461 cagtggcatc aggcaatagt gaccaaattt ggggtatagc ccacctgcga ccagctgcgg
     5521 ccaggtttca caaaattctt cgtaactgca atgcagcgta tcagggcgcg acaaaatgcg
     5581 gcgtaccgga ccgtgtgcca gaacagtttc gtcttcatat ttaatggggt gtctttttca
     5641 cgcgatgcgt aataagacgc acgttgagtc tgcgcttgcc acaacacaat agccgcgttg
     5701 tcggcgagcg cagaacgctt taccagttgc gaaagagcct cacacagcga ggccagatcg
     5761 ggctgctgca atagtgtccg agtgatgtcg aacaaccctt gctgtccgag atcactcatc
     5821 ggtgtatatg acattttgtt cttccaggaa agcaccgcaa agcggtgcaa aaggtcattg
     5881 actaactata ggagatttag aattttatta gcatatacgc ggaagaggtt cggcgtgtgg
     5941 taaatcgagg gttcgtttca caccatacag accggcaaga cgaacacctt tacgttccac
     6001 cacttcacca attagcgccg cgtctttccc cagtggatgg gaatgtaacg ctgccagcac
     6061 ttgctctgcc gcgttgcgtt caacggcgat caccagtttg ccttcgttgg caaagttaag
     6121 ggcgtccagt cccagcagtt cgcaaacgcc gcgcacggca ggtttaaccg gcagtgccac
     6181 ttctgaaatt tcaataccac aaccgcaggc tgccgcgaac tcatgaacta ccgcgttcac
     6241 accaccacgg gtggcatcac gcagcgcttt cacgccggga atgtcacgca gcgtctgaat
     6301 aagcggcgtc agtaccgcgc agtcgctgac cagttcgcca tccagcccca gctgctcacg
     6361 caggttaagg atagtcgccc cgtggtcccc gagcgtgccg ctaaccagta atacatcgcc
     6421 tgcggttagc gtctgtgcgc cccagtgaat attcgccgga attgcgccca tgccagcggt
     6481 gttgataaac agtttatctg ccgcgccgcg ctgcaccact ttagtatcgc cagtaacgat
     6541 ggcaatgcct gccgcgcgcg cggtttctgc catgctggtc actacggctt tcagtgtctc
     6601 catcggcaat ccttcttcga ggataaagcc acaggagaga tagcgcggaa tagcgccact
     6661 gaccgcaacg tcattggctg tgccgcaaat cgccagcttg ccgatattac cgccaggaaa
     6721 gaacagcggg tcaatgacgt aactgtcggt ggagaacgcc agacggtcgc cttctgctac
     6781 cagctgcgcc agatcaagac gtgcctggtc ttcctgctct gccagccacg ggttggcaaa
     6841 ggcttccata aacaggctgt tgattaattg ctgcatcgcc tggccgccgc tgccgtgggc
     6901 gagttggata ttattcacgc ttcattctcc tgctgacgat actgatacca cgcggcgcac
     6961 gctccttcgg aggagaccat cagtgcacca aatgcggttt gaggattaca ggtgttacca
     7021 aacagcgggc attgatgcgg cttacatttg cccgttaata cctcaccaca acgcgcgcgc
     7081 gggtcatcgc agacctgctg cggtgctggg cggaaatgtg cttcggcatc gaatcgttga
     7141 taatccggcg tcaggtgcac gccagaagat tcaatcacgc ctaagccgcg ccattcgctg
     7201 tcgccgttga cacagaacac atcagcaatt gcctgttgcg ccagcaggtt accggcatcc
     7261 ggtaccaccc gacgatactg attctctacc ttgctgtggg ccgctatttt ctgctccacc
     7321 agcatgacca cgccttgcag tagatcaagg ggttcgaaac cagcgaccac cagcggacga
     7381 tgaaaatcgc tggcgataaa attataggca tcagtgccga taaccatact aacgtggccc
     7441 ggcgcgagga acgcgtcgat accgttatcc ggctgttcca gcaaactgcg cagcgtcggg
     7501 ataagcgtaa tatgctggca gaagaagtaa aaattctgca catcgcgggc tttcgcctgt
     7561 tgcagggtga tggcggtggt cggcatggtg gtttcaaaac ctaagccgaa gaatacgact
     7621 ttacgggttg gattctcctg cgccagtttc aacgcatcca tcggcgagta aacgatgcgc
     7681 acatcggcac cgcgtgcttt tgcctgcaac agcgatccct gtttccccgg cacgcgcatg
     7741 gcgtcgccaa aggtacagaa gatgacttcc ggatggctgg caatctccac gcaggtgtcg
     7801 attctaccca tcggcagtac gcacaccggg caacccggac cgtggataaa ctcaacgttt
     7861 tcaggcagta actggtcgag gccgaattta aaaatggcgt gggtatgacc accgcacact
     7921 tccataatcc gcagagggcg ttcggcggtg taagagagat gtgaagcacg ttcgcgcaga
     7981 tgctcaatta actgcatcac ctgttccggc gcgcgatatt catcaacaaa acgcattatt
     8041 tttcctcgcc atacaacagc gcgccgacat ccggctcaac gtcaaacatg ttttgtaagg
     8101 cgtcgagagt gtcgcgtgct tcggcttcat taattacgct catggcaaag ccaacgtgta
     8161 ccagtaccca ctggcccacg cgcggctgac cgttttcatc gcagctgccg actaacgtta
     8221 aatcgacatc gcgctgaatg ccgcagacgt cgacttttgc ctggttgccg tcaatggtgc
     8281 ggatctggcc gggaacgcct atgcacatcg ctgtgtctcc agccagttca gccactggtc
     8341 catcccttcg ccgctggtgg cggaaataag gatgatttca atttctggat tgacttcgcg
     8401 ggcgcaggcg atgcacttct caacgtcaaa gttgagatac ggcaacaggt caactttgtt
     8461 gagcagcatc agcgaggcgg cggcaaacat atgcggatat ttcagcggtt tgtcttcacc
     8521 ttcggtaacg gaaagcaccg ccactttgtg tttttcaccg agatcgaagc tggccgggca
     8581 tacgaggttg ccaacgtttt cgataaacag aataccgtta tcgtccagtg gcagacgcgg
     8641 tgcggcgtcg gcaatcatct gtgcgtcaag atggcagcct ttaccggtgt tcacctgaat
     8701 cgctggtgtg ccggtagcgc gaatgcgtgc ggcatcgttc acggtttgct ggtcaccttc
     8761 aataaccgcg cacggaacgc tgtctttcag gcgcattagg gtttccgtca gcagggtggt
     8821 tttaccggaa ccagggctgg aaaccaggtt gagcaccagt tgcttgcggg cagcaaagcg
     8881 cgcgcggttg cgttcagcca gacggttatt tttgtccagc acgtcaattt cgacttccag
     8941 catccgacgc tggctcatgc ccggcgcgtg agtgcccgct tcgccatgac cgtaatgcag
     9001 gtcgccttct tcagtctggc tgggggtaaa ttcaggcgct ttaatgccgg tgattttcat
     9061 cttcgggcgt gccgccgggg caaatggcgc gctacgaaac gcggaatgag ggttatgttc
     9121 atcaccctcg atatacaggt tgccttcacc gcaaccgcat gttgtacaca tcgctcactc
     9181 ctggtctatt tctatccgcc gaatctgtaa accgtcgtct gccacaatct gtagcatgtc
     9241 accatgacac tgtggacagc ggcggacgcg ctgggtcagt agcgtcacat actgttggca
     9301 tgtttcacac cagcattcgg cctcttgttc ttcgaggtgc agtttacaac cttccgccac
     9361 gctgccgcgg caaaccagat caaaacaaaa ggcaagagag ctggtttcga cacaagaaaa
     9421 tgcgccaatt ttgagccaga ccccagttac gcgttttgcg ccgtgttttg cggcctgctg
     9481 ttcgatcaat tccagtgccc gttggcagag ggttatttcg tgcatatcgc ctcccattaa
     9541 ctattgccag ctacaagcaa taattgtgcc agtgttgatt atccctgcgg tgaataatgt
     9601 cgatgatgtc gaaatgacac gtcgacacgg cgacgaaatt catctttagc ttaaaaatct
     9661 atttaataac aataaattaa aagttggcat agaaaatgct taaagctggc atctctgtta
     9721 aacgggtaac ctgacaatga ctatttggga aataagcgag aaagccgatt acatcgcaca
     9781 gcggcattgt cgcctacagg accagtggca catctactgc aattcgctgg ttcaggggat
     9841 cacgttatcg aaagcgcgcc tgcatcacgc catgagctgc gcgccggaca aagaactctg
     9901 tttcgtcctt tttgaacatt ttcgtattta cgtcaccctg gcggatggct ttaacagcca
     9961 caccatcgag tattacgtcg aaacaaaaga tggcgaagac aaacagcgga ttgcgcaggc
    10021 gcaactgagc attgacggca tgattgatgg caaggtcaac atccgcgatc gcgaacaggt
    10081 tctggaacac tatctcgaaa aaatcgctgg cgtttacgac agcttataca ccgctattga
    10141 aaacaatgtg ccggtgaatt taagccaact ggtaaaggga caaagcccgg cagcatgagc
    10201 tgaggctttg cccgttttgc aggcgttacg cctgtttggg gatgggcgtg tcgatgagtg
    10261 tcgaaaatga catttcatcg gcatgttttc gtcaaaaatg acaatcacct gaggaatgcc
    10321 tggtgaatcg ttttgtaatt gttgactcca cgctctgtat cggctgccac acttgtgagg
    10381 ccgcctgttc agagacgcat cgccagcacg gcctgcaatc aatgccgcgc ctgagagtga
    10441 tgctgaatga aaaagaatct gcgccgcagc tctgtcacca ctgtgaagat gcaccctgtg
    10501 cggtggtttg cccggttaac gccatcaccc gcattgatgg ggccgtgcag ttgaatgaaa
    10561 gtctgtgcgt aagctgcaaa ctgtgcggca tcgcctgccc gtttggcgca attgaatttt
    10621 ccggcagccg tccgctggat attccggcaa acgccaatac cccgaaagcg ccaccggcac
    10681 cgcctgctcc ggcgcgtatc agtacgctgc ttgactgggt gccgggtatt cgcgcgatcg
    10741 ccgtcaaatg tgacctttgc agttttgatg aacaaggtcc ggcctgcgtg cggatgtgcc
    10801 cgactaaagc cctgcatctg gtggataaca ccgatatcgc ccgcgtcagc aaacgtaagc
    10861 gtgagctgac ctttaacacg gactttggcg atctcacctt gtttcagcag gctcaaagtg
    10921 gagaggctaa atgagcgcaa tttccctgat caatagcggc gtggcatggt ttgtcgccgc
    10981 cgctgttctg gcatttctct tttcttttca aaaagcgtta agtggctgga tagctggaat
    11041 tggcggcgcg gttggtagtc tgtatacggc agccgcgggc ttcactgtac tgactggcgc
    11101 ggttggcgtg agcggtgcgc tgtcgctggt aagctacgat gtgcaaatct ctccgcttaa
    11161 cgcgatttgg ctgattacgc tcggtctgtg cggtctgttt gtcagcctct acaacattga
    11221 ctggcatcgc cacgcgcagg tgaagtgcaa cggcttgcag atcaatatgt tgatggctgc
    11281 cgccgtctgc gccgtcatcg ccagcaacct cggcatgttc gtggtaatgg ccgaagtcat
    11341 ggccttgtgt gcggtgttcc tcaccagcaa cagcaaagag ggcaaactgt ggtttgcgct
    11401 ggggcgtctt ggcactctgc tgctggtgat tgcttgctgg ctgctgtggc agcgttatgg
    11461 cacgctggat ctgtgcctac tggatatgcg tatgcaacag ctgccgctcg gttccgatat
    11521 ctggctgctc ggtgtgattg gctttggcct gctggccggg attattccgc tgcacggctg
    11581 ggtgccgcag gcacatgcta acgcctctgc gccagctgcc gcgttgtttt ctacggtagt
    11641 catgaaaatt ggcctgctgg gcatgttaac cctgtcactg ctgggcggta atgcaccgct
    11701 gtggtggggg atcgcgctgc tggtgctcgg catgatcacc gcgttcgtcg gtggtctgta
    11761 tgcgttgatg gaacacaaca tccagcgcct gctggcttac cacaccctgg aaaatatcgg
    11821 cattatcctg ctggggctgg gcgctggcgt aacgggtatc gcgctcgaac aaccggcgct
    11881 gattgctctt ggcctggtcg gtggtctgta ccatctgctt aaccatagcc tgttcaaaag
    11941 cgtactgttc ctcggggcgg ggagcgtctg gttccgtacc ggtcatcgcg atatcgaaaa
    12001 actcggtggt attggcaaga aaatgccggt tatctccatc gccatgttag tcgggttgat
    12061 ggcaatggct gcgctgccgc cactgaacgg gtttgccggg gaatgggtta tctatcaatc
    12121 cttcttcaaa ctgagcaata gcggcgcgtt tgttgcccgt ctgctggggc cgctgctcgc
    12181 tgtgggtctg gcaattaccg gtgcgctggc ggtgatgtgt atggcgaaag tctatggcgt
    12241 cacgttcctc ggcgcgccgc gtaccaaaga agccgaaaac gccacctgtg cgccgctcct
    12301 gatgagcgta agcgtagtgg cactggcgat ttgctgcgta attggcggtg ttgctgcgcc
    12361 gtggctgctg ccgatgctct ctgctgctgt acctctgcct ctggagcctg ctaacaccac
    12421 cgtttctcaa ccaatgatca cattgctgct gattgcctgc ccgctgctgc cattcatcat
    12481 tatggcgatt tgcaaaggcg accgcctgcc gtcacgttcc cgcggtgcgg cctgggtgtg
    12541 cgactacgac catgaaaaat caatggtgat taccgctcac ggttttgcca tgccggttaa
    12601 acaggcgttt gcgccagtgc tgaaactgcg caaatggctg aatccggtgt cgctggtgcc
    12661 gggctggcag tgcgaaggta gtgcgttgct gttccgccgg atggcgctgg ttgaactggc
    12721 ggtactggtg gtgattattg tttcacgagg agcctgagaa tgagtgtttt atatccgtta
    12781 attcaggcgc tggtgttatt tgccgttgcg ccgctgctct ccggtattac ccgcgtggcg
    12841 cgcgcccgtc tgcataaccg tcgtgggccg ggtgtgttgc aggagtatcg tgacattttc
    12901 aaactgctgg gccgccagag cgttggcccg gatgcctccg gctgggtgtt ccgcctgacg
    12961 ccgtatgtga tggtgggcgt catgctgact atcgctactg cgctgccggt ggtgaccgtc
    13021 ggttctccgc tgccgcaact gggtgattta atcaccttac tgtatctctt tgccatcgcg
    13081 cgtttcttct ttgccatttc tggtctggat accggtagcc cgtttaccgc tatcggcgcg
    13141 agccgtgaag cgatgcttgg cgtgctggtc gaaccgatgc tgctgcttgg tctgtgggtt
    13201 gccgcacagg ttgccggttc caccaacatc agcaacatca ccgacaccgt ttatcactgg
    13261 ccgctgagcc agagcatccc gctggtactg gcgctttgtg cctgtgcgtt cgccaccttt
    13321 atcgaaatgg gcaaactgcc gttcgacctg gcggaagccg agcaggagct gcaggaaggc
    13381 ccgctctctg aatacagcgg cagcggcttt ggcgtcatga aatggggtat cagcctgaaa
    13441 cagctggtgg tgctgcagat gttcgtcggc gtatttatcc cgtggggaca gatggaaacc
    13501 ttcaccgccg gtgggctgct gctggcgctg gtgattgcca tcgtcaaact ggtcgtcggc
    13561 gtcctggtta tcgcgctgtt cgaaaacagc atggcccgtc tgcgtcttga tattactccg
    13621 cgcattacct ggactgggtt tggctttgca tttttagcgt tcgtctcctt gctggcggcg
    13681 tgattaaaga gagtttgagc atgtctgaag aaaaattagg tcaacattat ctcgccgcgc
    13741 tgaatgaggc atttccgggc gtcgtgctgg accacgcctg gcagaccaaa gatcagctga
    13801 ctgtcaccgt aaaggtgaac tatctgccgg aagtggtgga gtttctctac tacaagcagg
    13861 ggggctggct gtcggtgctt tttggtaacg acgaacgcaa actgaatggt cattacgccg
    13921 tttattacgt gctgtcgatg gagaagggga ctaagtgctg ggtaacggtt cgcgtcgaag
    13981 ttgatgccaa caaaccggag tatccgtccg tgacgccgcg cgttccggcg gcggtgtggg
    14041 gcgagcgcga agtgcgcgat atgtacggtt tgattccggt tggtctgccg gatgaacgcc
    14101 gtctggtgct gccggatgaa ctttatccgc tgcgtaaaga cagcatggat tatcgtcagc
    14161 gtccggcacc gaccaccgat gctgaaacct acgagttcat caacgaactg ggcgacaaga
    14221 aaaacaacgt cgtgccgatt ggtccgctgc acgtcacttc tgacgaaccg ggccacttcc
    14281 gtctgttcgt cgatggcgaa aacattatcg acgccgacta ccgtctgttc tacgttcatc
    14341 gcggcatgga aaaactggcg gaaacccgta tgggttacaa cgaagtgacc ttcctctctg
    14401 accgtgtgtg cgggatctgc ggctttgccc acagcaccgc ctacaccacg tcggtagaaa
    14461 acgcgatggg tattcaggtg ccagaacgtg cgcagatgat ccgcgccatt ctgctggagg
    14521 tggaacgcct gcactcgcat ctgctcaacc tcggtctcgc ctgccacttt accggctttg
    14581 actccggctt tatgcagttc ttccgcgtgc gtgaaacctc catgaaaatg gcagagatcc
    14641 tcaccggtgc gcgtaaaacc tacggcctga acctgatcgg cgggattcgt cgcgatctgt
    14701 tgaaagatga catgatccag acccgccagc tggcgcaaca gatgcgtcgt gaagtgcagg
    14761 agctggtgga tgtgctgctg agtacgccga acatggaaca gcgcaccgtc ggcattggtc
    14821 gtctggaccc ggaaatggcc cgcgatttca gtaacgttgg cccgatggtt cgcgccagcg
    14881 gtcacgcccg cgatactcgc gccgatcacc cgtttgtcgg ttatggcctg ctgccaatgg
    14941 aagtccacag cgagcagggc tgcgacgtta tttcccgtct gaaagtgcgt atcaacgaag
    15001 tttataccgc gctgaacatg atcgactacg gtctggataa cctgccgggc ggtccgctga
    15061 tggtggaagg ctttacctac attccgcacc gtttcgcgct gggctttgcc gaagcgccgc
    15121 gcggcgacga tatccactgg agcatgaccg gcgacaacca gaagctgtac cgctggcgct
    15181 gccgtgccgc gacctacgcg aactggccga ccctgcgcta catgctgcgc ggtaacaccg
    15241 tttccgatgc gccgctgatt atcggtagct tggacccttg ctactcctgt accgaccgca
    15301 tgaccgtggt cgatgtgcgt aagaaaaaga gcaaagtggt gccgtacaaa gaactcgagc
    15361 gttacagcat tgagcgtaaa aactcgccgc tgaaataagg aatcgccatg tttaccttta
    15421 tcaaaaaagt catcaaaacc ggcacggcga cctcgtctta tccgctggag ccgattgcgg
    15481 ttgataaaaa cttccgtggt aagccggagc agaacccgca gcagtgcatc ggctgtgcgg
    15541 cctgcgtcaa tgcctgcccg tcaaacgcct taacggttga aactgacctc gccaccggtg
    15601 agctggcgtg ggagtttaat ctcgggcgct gcatcttctg tggacgctgc gaagaagttt
    15661 gcccgacggc ggcgatcaaa ctgtcgcaag agtacgaact ggcagtgtgg aagaaagaag
    15721 acttcctgca acagtcccgc ttcgcgctgt gcaactgccg cgtctgcaat cgtcctttcg
    15781 ccgtccagaa agagatcgac tacgccattg cgctgcttaa gcacaacggc gacagccgcg
    15841 cggaaaacca ccgcgaaagc tttgagactt gcccggaatg taagcgccag aaatgcctgg
    15901 tgccgtccga ccgtattgaa ctgactcgcc atatgaaaga ggccatctga tgagcaattt
    15961 attaggcccc cgtgacgcca acggcattcc ggtcccgatg acggtggatg aatccatcgc
    16021 cagcatgaag gcgtcgttac tgaaaaaaat caaacgttct gcctacgttt atcgcgtgga
    16081 ctgcggcggc tgcaacggct gcgaaatcga aattttcgcc acgctttcgc cgctgtttga
    16141 tgcagaacgc ttcggcatta aagtcgttcc ttcaccgcgt catgcggata ttttactgtt
    16201 taccggcgcg gtcacccgtg caatgcgatc ccctgcgctg cgtgcgtggc agtccgcgcc
    16261 ggacccgaaa atttgtatct cctacggtgc ctgcggtaac agcggtggta ttttccacga
    16321 tctctactgc gtgtggggcg gtacggataa aatcgtcccg gtagatgtct acattcctgg
    16381 ttgcccgcca acgcctgccg ccacgctgta cggctttgca atggcgctcg gcctgctgga
    16441 gcagaaaatt cacgcccgtg gcccgggtga gcaggatgaa caacaggcgg aaatcctgca
    16501 tggcgatatg gtgcagccgc tgcgtgtgaa agtggatcgc gaagcacgcc gcctggcggg
    16561 ttatcgttac ggtcgtcaga ttgccgatga cttcctgaca cagttagggc agggcgaaga
    16621 acaggttgca cgctggctgg aagcggaaaa cgatccgcgt ctgaacgaga ttgtcagcca
    16681 tctgaatcat gttgttgaag aggcgcgtat ccgatgagtg aaaaggtggt gttcagtcaa
    16741 ctgagccgta aatttattga tgagaacgat gccacgcccg ccgaggcgca gcaggtggtc
    16801 tattacagcc tggcgattgg tcaccacctt ggggttatcg attgcctgga agcggcgctc
    16861 acctgcccgt gggatgaata tctggcatgg attgccactc tggaggcggg cagtgaagcc
    16921 cgccgcaaaa tggaaggcgt gccgaaatat ggtgagatcg tcatcgacat taaccatgtg
    16981 ccgatgctgg ccaacgcatt cgataaagcc cgggcagcgc aaacttcgca gcagaaggaa
    17041 tggagtacaa tgctgttaag tatgctgcat gatattcatc aggaaaacgc catctatttg
    17101 atggtgagga gactgcgtga ctgacgtttt actctgtgtt ggcaatagca tgatgggcga
    17161 tgatggcgct ggcccgctgc tggcggaaaa gtgcgccgcc gcgccgaaag gtaactgggt
    17221 ggtgattgat ggcggtagtg caccggaaaa cgacatcgtc gccatccgtg aactgcgccc
    17281 tacgcggtta ctgattgttg acgctactga tatggggcta aaccccggcg aaatccgcat
    17341 catcgacccg gatgatatcg ccgagatgtt tatgatgact acccataaca tgccgctgaa
    17401 ctatcttatc gaccagttga aagaagacat tggcgaagtg attttcctcg gcatccagcc
    17461 ggatatcgtc ggcttttact acccgatgac tcagccgatt aaagatgcgg tagaaaccgt
    17521 ttatcaacga ctggaaggct gggaagggaa tggcggcttc gcgcagttag cggtggaaga
    17581 agaataattc tccatcaggg aattcagcga aatgaatatc agaacaggga tgtgcgcttt
    17641 tttattgagc ttagtgctgc ctgcacaggc caccagcttt accgaatatc tgccaatgag
    17701 tgacagcgaa tacgcgcaaa agcgggcgct gaaacctctc ctgaccatgc cttatgatgc
    17761 cgagcaaacc tggcatttta gaaaagtggg cgttgctggc gtgacgcttg aaaagatgcc
    17821 aaatgatgat agtgaatggc aattgaacgg taaagatcgc gccggtaaat cctggtctgt
    17881 gcctgtgggt atgctggcaa acatagcggg caaagggcaa ctctatcgcg ccgatcttga
    17941 tcgtaacggc attcaggatc tggttatctg gttaccaagt accggtaatg gtctggctcc
    18001 ttatgcacat ttaattctta tgacgttcac gcgcgaaggc cgtccctgtg tctttgaacc
    18061 caggggattc tacaccgcaa gcaaaactgg cgtagatgat ttgctggatt tgcaaggcaa
    18121 tggtcacacg caattgctgg atatgcagtt tgattccggt tactggataa ccagtctgta
    18181 tcaggtaaaa gacgccaaat ggcagcgtgt tcacggctgg tttggcaagt taagctatcc
    18241 cgcgctcacg cgttttactt atacccccaa ccgtaaactc gtcctcaaac ccatcgccgg
    18301 gcgcgacccg caaacggaag atctggcgca gacgcagcgt tgcttgataa agggggatgt
    18361 tcttgatggg gtgaaccaga actgactttt tgcagagtgg ctatgaggat tttgggggta
    18421 attatatcgc gctcaagtgt ttacatattg tgaacttgga tttgatgagc tatagtttcc
    18481 gaacacataa ggatagaact cgcagatgtg gctgtttact tactataagg aagttaaaac
    18541 ttaaaaatgc acatcatttc taaagcgcca tttgaagagt ctgcgcgtaa atatcctaac
    18601 gacgctctgg ctcttcaggc attgtatcgg gttattaagg aaacggattt ttctacgcca
    18661 gaagagatgc gaacggcgtt tcccaacctc gacaatttta aatatcgcaa caaatggtac
    18721 gttctggatg taggtgggaa caacctaagg gtgattgcct acataaactt tgttaataag
    18781 cggttctttg tgaagcacat aactaatcac gccgagtatg acaaactgac acgttactac
    18841 agagagaaca aagaatgact gctaatgctg cacgcgctgt taaagcaaca agagaactgg
    18901 tcaatgcagt cccctttctg ggaggtagtg actcagaaga tgattatcgt gaagcactgg
    18961 aactggtcga atatttgatt gaagaagatg acaccaatcc acttatcgac tttctggcga
    19021 gccgcattgc agaatatgaa aacaacaacc aagagtttgc agagttcgac aaagccgttg
    19081 cggcaatgcc tgttggcgtg gcgttacttc gcacgctgat tgatcaacat aatctgacct
    19141 atgcggattt gaagaacgaa atcggctcta aatccctggt cagtcagatt ttgtctggac
    19201 aacgatccct gacgatatcc cacattaagg cactttcagc ccgatttggc gttaagccgg
    19261 aatggtttct ttgattttga ctgatgccaa accccagatt gcccgatgcg ctgcgcttat
    19321 cgggccttcc tggttcgtgc gacatgtagg ccggataagg cgttcacgcc gcatccggca
    19381 ctgttaccta ctctaaatct tccccattac tggcaatcac ttttttatac caccagaacg
    19441 atttcttacg tgtgcgcgtc agcgtgccgt tgcctgcgtc gtcacggtct acgtagacaa
    19501 aaccgtagcg tttgctcatt tcacccgtag aggcggagac taaatcaata cagccccagg
    19561 tggtgtagcc catcagcgga atgccatctg caatcgcttc gcccattgcg cggatatgtt
    19621 cgcgtaaata gctgatgcga tagtcatcgt taatctcgcc attggcagca agttcatctt
    19681 ttgcgccgag gccgttttcc accagaaaca gcggcttctg ataacggtcg tacatcatgt
    19741 tcatggtgat acgcagaccg agtggatcaa taccccagcc ccagtcgctc acttgcagat
    19801 acgggttacg cagcgatttc actacgttcg ccgcactgct attgttggcg ttcatctccg
    19861 ccgaggcgca gcgcgaggcg taatagctga aagagacaaa atcgacggtg tttttcagga
    19921 tttcatcatc gcccggtgct ttgtcgatgg ttaccccttt ttcgcggaat acgcgggcag
    19981 agtaagccgg atacgtgccc cgcgcctgca catcgataaa aaacaggttt tcccggtctt
    20041 tctccagcgc cgcccagaca tcttccggct tgcaactgta agggtagaag ttaccgcccg
    20101 ccagcataca gcccacctga ttttgcgggt taacctcatg ggcgattttg gtggctagcg
    20161 cactggcaac cagctggtga tgcgcggcct gatatttcac ctgatcctga ttttcacctt
    20221 cttcaaacac cagacccgcg ccggagaacg ggctatgcaa cataatgttg atttcattga
    20281 aggtaagcca gtatttcacc agaccatcaa atgcttcaaa gcaggtccgg gcgtagcggc
    20341 tgaaaaactc caccagcttg cggttacgcc aggagccata ttcggtgacc agatgcatcg
    20401 gcacatcgaa gtggcacaac gtgaccagcg gttcgatacc gtacttttta cactcttcaa
    20461 agacagaacg ataaaaagca atgccctgct gattgggcgt gatttcatcg ccctgcggaa
    20521 agagacggct ccaggcaatt gaggtacgga aaaccttgaa tcccatctct gccatcaggg
    20581 cgatatcttc tttataacga tgataaaaat ccgtcgcctc atggctggga taaaactcgt
    20641 catcgcgcaa ctgaaaacgt ttttccagcc ccagtttcac cgccattcga tgctcgccgt
    20701 gtgggatcat atcgacagtg gtcagccctt tgccaccttc acggaacgca ccttcagact
    20761 ggttggcggc aagcgcgccg ccccataaaa aaccttctgg aaatactgac attttcatcc
    20821 tcaattaaga cttacttctt tggcgacggt cggttgtacg ctctgatgct ttcgcgcctc
    20881 ggcagccgct tcctcaacag gaatatcctc aaagccgagc aacagtgtga ggataaacga
    20941 cagcaccacc gccagcgcca tgaccgcgaa cacccagacg atgctcattg gattcgccgg
    21001 atcgaagaat tgcacgctgg taaataaccc tggcgctgcc attgagtggc tggcaagccc
    21061 cgccataccg gcaaccgcgc cgcaaataaa accgctgata agactggcga taagcggacg
    21121 tttcaggcgg atcgccacgc cgtataacgc cggttcggaa atccccgcca taatggctga
    21181 tgccgccgca gccagcgccg tctggcgcag ttccgggttt ttcgttttcc acgccaccgc
    21241 cagtgatgaa ccgcccagcg acaggttagc gccgatctct gacggcatga ccatcccttc
    21301 tttgccggtt tcggcaatgg tctgaatgat ggttggcgta aagacgcggt gcatcccggt
    21361 cattaccagc agaggccaca gcgcgcccat aatggcgact gaaagccagc ccagataacc
    21421 atgaatggtg taaaccagcg ccgaaatggc gctaccgatc cagataccaa tcgggccaat
    21481 cagcaggatt gccagcggtg cggcaatcaa cacaatcagc attggcttga ggaagttttt
    21541 ggtcaccgcc ggtgtaatgc gatccaccca gcgttcgata tatgacaggc accaggtcat
    21601 caccagcgcc gggatcactg tgtaggtgta tttcaccgcg gtgaccggaa tcagggcaaa
    21661 ttcgacatgt tcaccctggg ccgctttcgc catcagttca ataaaactcg gatgtaccag
    21721 cacacccgcg atggcaatcg ccagcgacat attggtttta aatttgatgg cggcagatgc
    21781 cgcgaccatc agcggcagga agaagaaagc accgtcacca atcacattca gaatggttaa
    21841 ggtcggcgat ccttttgtca gcacgccgct catctcgagg atcatcgcca gcagtttgac
    21901 catcgatccg ccgataatcg ccgggatcag cggtgacatg gtgccgatca gcgcatcgag
    21961 gatccccgca ccaatgcgac gcagcgtgag tttgggttta cccacgggct gtgcgggctg
    22021 catatctccc ggcagcaggc tgacgacttc ctgaaaggct tgtgaaacgg tattgccgat
    22081 aatcacctga cactggttgt cactacgtac cacgccgagc acgccgggga tggtttttaa
    22141 cgtcgggctg tcgataagct ggtcatcttt gataacaacg cgcaaacgcg tcatacagtg
    22201 cgtgaccgcc gagatgttat caacgccgcc cagtgccgct atcaccgagc gtgccagcgc
    22261 cgcataattt ttggccatcg gattttatcc tgttatcagt agggtagatg tagttgttat
    22321 cagtcgacgt aacggaaaaa tgccgatgct gaaacggaat taggaaaccg gtttcacaaa
    22381 atcatgaata gttgtcacta aataagcaag agagttaatt gttcattttt tatatttgtg
    22441 aaaccggtca cctgaatatt tttcccgccg attgcgcaag gacaatcggc aaacggtgca
    22501 gtacactgcg ttgattgcgt gtaaagggat gaaaatgatg acgacgatgc tggaagtggc
    22561 gaagcgcgcc ggggtttcaa aagcgaccgt ttcccgcgtg ctttcaggta atggctacgt
    22621 cagccaggag actaaagatc gcgtgtttca ggcggtagaa gagagcggtt accgcccaaa
    22681 cttgctggcg cgcaatctgt cggcgaagag tactcagacg ctggggttgg tagtgaccaa
    22741 cacgctttac cacggcattt attttagtga attactcttt catgccgcgc gaatggcgga
    22801 agagaaaggg cggcagttgc tattggcaga tggtaaacac agcgccgaag aagagcgcca
    22861 ggcgattcag tatctgctgg atttgcgctg cgacgccatt atgatttacc cgcgtttttt
    22921 aagcgtggat gagatcgatg acatcattga cgcgcacagt cagccgataa tggtgcttaa
    22981 tcgccgcctg cgcaaaaaca gcagccatag cgtctggtgc gatcataaac agaccagctt
    23041 taatgccgtg gcagagttga taaacgccgg acatcaggag attgctttcc ttaccggctc
    23101 gatggattcc cccaccagca ttgaacgtct tgctgggtat aaagacgcgc tggcgcagca
    23161 tggtattgcg ctcaatgaaa aacttatcgc taacggtaaa tggacgcccg ccagcggggc
    23221 cgaaggggta gaaacgttgc tcgaacgtgg ggctaaattt agcgcgttag ttgccagtaa
    23281 cgacgatatg gcgataggtg cgatgaaagc gttgcacgag cgcggcgtag cggtgccaga
    23341 gcaggtgtca gttatcggat tcgatgatat cgcaattgcc ccctatatcg ttccggcgct
    23401 ctccagcgta aaaattccgg tgactgagat gattcaggaa attattggac ggctgatttt
    23461 tatgctcgat ggcggggatt tctcaccgcc gaaaaccttc agcggaaaac tgatccgccg
    23521 cgactcactc attgctcttt cgcgataaac attgtcatca catgtcatcg tcactagtgt
    23581 cgatgacatc ttttttcctg cattacagaa attaatttcc ttaaataaca gtgaattaaa
    23641 actggcatga tttgtgaatg tatcggcgca ttaactgtca ttgctggaga atttgatgaa
    23701 ccgtttcatc attgctgacg cgagtaaatg tattggttgc tgtacctgtg aagtggcgtg
    23761 cgtggtttct catcaggaaa atcaggactg tgcatcgctg accccggaaa catttttacc
    23821 gcgtatccat gtcattaaag gtgtgaacat ttccacggcg acagtttgcc gtcagtgtga
    23881 agatgcaccg tgcgctaacg tctgcccgaa tggtgctatc agccgtgata aagggtttgt
    23941 tcatgtgatg caggaacgtt gcattggttg caaaacctgc gttgtggctt gcccgtatgg
    24001 cgcgatggaa gtggtggtac gtccggtgat tcgcaacagc ggcgcggggc tgaatgtacg
    24061 ggctgacaaa gccgaagcca ataaatgtga cctgtgcaac catcgtgaag acggcccggc
    24121 gtgtatggcg gcttgcccga ctcatgcgct gatttgtgtc gatcgtaata aacttgaaca
    24181 actgagtgca gaaaaacgcc gccgtacggc gctgatgttc taaaaatctt acagaactgt
    24241 tctggcatca aaggaattcc cggaaatatt cgtagaatac ggagctggcg gtatcgccga
    24301 cctgagact
//
LOCUS       DAZPAC010000047        24271 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZPAC010000047 DAZPAC010000000
VERSION     DAZPAC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.047"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            158..295
                     /locus_tag="ACHUPM_001559"
                     /pseudo
     CDS             158..295
                     /locus_tag="ACHUPM_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(403..1638)
                     /locus_tag="ACHUPM_001560"
     CDS             complement(403..1638)
                     /locus_tag="ACHUPM_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0869379.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1785..1861)
                     /locus_tag="ACHUPM_001561"
     tRNA            complement(1785..1861)
                     /locus_tag="ACHUPM_001561"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1825..1827),aa:Pro,seq:tgg)
     gene            complement(1904..1990)
                     /locus_tag="ACHUPM_001562"
     tRNA            complement(1904..1990)
                     /locus_tag="ACHUPM_001562"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1954..1956),aa:Leu,seq:cag)
     gene            complement(2011..2086)
                     /locus_tag="ACHUPM_001563"
     tRNA            complement(2011..2086)
                     /locus_tag="ACHUPM_001563"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2051..2053),aa:His,seq:gtg)
     gene            complement(2145..2221)
                     /locus_tag="ACHUPM_001564"
     tRNA            complement(2145..2221)
                     /locus_tag="ACHUPM_001564"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2185..2187),aa:Arg,seq:ccg)
     gene            complement(2322..3707)
                     /locus_tag="ACHUPM_001565"
     CDS             complement(2322..3707)
                     /locus_tag="ACHUPM_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0869380.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3898..4638)
                     /gene="wecG"
                     /locus_tag="ACHUPM_001566"
     CDS             complement(3898..4638)
                     /gene="wecG"
                     /locus_tag="ACHUPM_001566"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR0869381.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4641..5993)
                     /gene="wzyE"
                     /locus_tag="ACHUPM_001567"
     CDS             complement(4641..5993)
                     /gene="wzyE"
                     /locus_tag="ACHUPM_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR0869382.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5990..7069)
                     /gene="rffT"
                     /locus_tag="ACHUPM_001568"
                     /gene_synonym="wecF"
     CDS             complement(5990..7069)
                     /gene="rffT"
                     /locus_tag="ACHUPM_001568"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR0869383.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7066..8316)
                     /gene="wzxE"
                     /locus_tag="ACHUPM_001569"
     CDS             complement(7066..8316)
                     /gene="wzxE"
                     /locus_tag="ACHUPM_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR0869384.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8318..9448)
                     /gene="rffA"
                     /locus_tag="ACHUPM_001570"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8318..9448)
                     /gene="rffA"
                     /locus_tag="ACHUPM_001570"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR0869385.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9453..10127)
                     /gene="rffC"
                     /locus_tag="ACHUPM_001571"
                     /gene_synonym="wecD"
     CDS             complement(9453..10127)
                     /gene="rffC"
                     /locus_tag="ACHUPM_001571"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR0869386.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10105..10986)
                     /gene="rfbA"
                     /locus_tag="ACHUPM_001572"
     CDS             complement(10105..10986)
                     /gene="rfbA"
                     /locus_tag="ACHUPM_001572"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR0869387.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11005..12072)
                     /gene="rffG"
                     /locus_tag="ACHUPM_001573"
     CDS             complement(11005..12072)
                     /gene="rffG"
                     /locus_tag="ACHUPM_001573"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR0869388.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12072..13334)
                     /gene="wecC"
                     /locus_tag="ACHUPM_001574"
     CDS             complement(12072..13334)
                     /gene="wecC"
                     /locus_tag="ACHUPM_001574"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR0869389.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13331..14461)
                     /gene="wecB"
                     /locus_tag="ACHUPM_001575"
     CDS             complement(13331..14461)
                     /gene="wecB"
                     /locus_tag="ACHUPM_001575"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR0869390.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14517..15563)
                     /gene="wzzE"
                     /locus_tag="ACHUPM_001576"
     CDS             complement(14517..15563)
                     /gene="wzzE"
                     /locus_tag="ACHUPM_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR0869391.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15575..16678)
                     /gene="wecA"
                     /locus_tag="ACHUPM_001577"
     CDS             complement(15575..16678)
                     /gene="wecA"
                     /locus_tag="ACHUPM_001577"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR0869392.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16918..18177)
                     /gene="rho"
                     /locus_tag="ACHUPM_001578"
     CDS             complement(16918..18177)
                     /gene="rho"
                     /locus_tag="ACHUPM_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR0869393.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18262..18363)
                     /gene="rhoL"
                     /locus_tag="ACHUPM_001579"
     CDS             complement(18262..18363)
                     /gene="rhoL"
                     /locus_tag="ACHUPM_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR0869394.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18504..18833)
                     /gene="trxA"
                     /locus_tag="ACHUPM_001580"
     CDS             complement(18504..18833)
                     /gene="trxA"
                     /locus_tag="ACHUPM_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR0869395.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18964..20229
                     /gene="rhlB"
                     /locus_tag="ACHUPM_001581"
     CDS             18964..20229
                     /gene="rhlB"
                     /locus_tag="ACHUPM_001581"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR0869396.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20363..21847
                     /gene="gppA"
                     /locus_tag="ACHUPM_001582"
     CDS             20363..21847
                     /gene="gppA"
                     /locus_tag="ACHUPM_001582"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR0869397.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21894..23915)
                     /gene="rep"
                     /locus_tag="ACHUPM_001583"
     CDS             complement(21894..23915)
                     /gene="rep"
                     /locus_tag="ACHUPM_001583"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR0869398.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24132..>24271
                     /locus_tag="ACHUPM_001584"
     CDS             24132..>24271
                     /locus_tag="ACHUPM_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869399.1"
                     /translation="MAINFSPKVGEILDCNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLV"
BASE COUNT         6098 a         6650 c         5996 g         5527 t
ORIGIN      
        1 aaaacgctct cctgattttc caattcattt tctggataaa taaataattt atttttgtca
       61 ctattattta tgtaatcatc ctgtcaggga gagggatctc aattatcaat gcttaattac
      121 gtcatcattt tgatgacatg aaaccggagc attttgcatg gaattttcgt tttcacccaa
      181 acttcttgtt gttgctgtcg ccgccgctct tcctctcatg gccagcgcag cagatacccc
      241 gtcaactgcc accgcacgca aaggctttgc cggatacgat cacccaaacc agtatccaca
      301 gaacgctgca atttattgaa tttgcacgat catgtaggcc ggataaggcg tttacgccgc
      361 atccggcaat caaccgcagg cggccacaaa atggccgctt ttttacttat tcaccaccag
      421 caaatgtgcc tgcataatgt cactggccgg gcgaccgtgc gccagcaaat cagccattgc
      481 tttaagatat ggcggtagat ggcggaaata acgctgatac ccggcacaca aataattcag
      541 ccccggtttg ccgctggcat cgagcatgaa gcggtgtttc gggcagcctc cccagcacgc
      601 ttttaacacg ttacaactgc gacactgcgc cggtaattgc ttaaatttat cttcaccaaa
      661 cacctgctgt tgcggggaat cgatcatttc tgcaattgtt tgctgatgca tattccccag
      721 ccgatattgc ggataaacat agtgatcgca ggcgtaaacg tcgccgttgt gctcaacaat
      781 caccgagcgc ccacaggttg gctgatgatg gcaaaccgca cccggcgcac cgacaaaatt
      841 ggcaaacgcc cattcgatat tcatcacgaa aatcttgccg acgtcgcgtt tgatccagtg
      901 gtcgaatatc gccaccagaa attcaccaaa ttcatcgggg cgcaccgacc attccgtcag
      961 ttccccctga atatcaccag gcgcatgcaa tttcagtcca tcgcgggcag ctgtttcatc
     1021 agccagacgc tcgaccaccg gaataaactg gatgaattcg actcccgcat cgcacaaaaa
     1081 atcataaacc tgcaacggtt gctgcgcgct ggtgcggttg acgcagacca gcacgttata
     1141 gtcgacatga tgtttttgca gcagcgtcag ggcacgcatc accagcttat gcgttggtct
     1201 gccacctttg gtcacgcgat attgattgtg gatctcagcc ggaccgtcca gcgataaccc
     1261 aacaagaaaa tgattttctg ccagaaatgc acaccattta tcatcgagca gcacgccgtt
     1321 agtctggaag ctgttactta tcttcctgcc agcaccgtat ttcgcctgta actttacggc
     1381 acggcggtaa aaatccagcc cgagtaacgt tggttcgccg ccctgccagg taaaagccac
     1441 ttcgttttgc ggttcgctgg cagcgatata gtgacggaca tacgcttcca gcgtgtcatc
     1501 gtccatatgc gtgactggct tttcgcggta aagggattgt ttttcgagat aaaaacagta
     1561 gtcacagttc agattacaat cggaaccact cggtttcgcc atcacatgaa aagcacgcgt
     1621 tggaacctgt tgcagcatgg tcactccttg cctgaagtca tccttcaata cgttaagggc
     1681 gaaatcatac cgtggggcaa tgagttatta cgggcgttta ttgcgaaggg agggtgacga
     1741 aatgcacaga aaacaaaaaa ccccgccgaa gcggggttca aaattggtcg gcgagagagg
     1801 attcgaacct ccgacccact ggtcccaaac cagttgcgct accaagctgc gctactcgcc
     1861 gaaatactgc tttttgaatt tttagttcaa ttctttaaag tcgtggtgcg agggggggga
     1921 cttgaacccc cacgtccgta aggacactaa cacctgaagc tagcgcgtct accaattccg
     1981 ccaccttcgc attgtcacaa cttctaataa tggggtggct aatgggattc gaacccacga
     2041 caactggaat cacaatccag ggctctacca actgagctat agccaccact acaaatcttg
     2101 ttacgcggta ttactaccac cgcagctcaa gcgccgggac taaatggcgc gcccgacagg
     2161 attcgaacct gagacctctg cctccggagg gcagcgctct atccagctga gctacgggcg
     2221 cttagcgccg ttgcgggggg ataatacgga cttcccaccc ctctgtctag tcccttttta
     2281 aataaattgc gcgtttggtt acggtttgtg cgttttgctg cttattcctc cagtttatgc
     2341 gctttcccgt ggcgattaag gccaaaaact ttataaatcg ccgtcaccgc cagcataaag
     2401 atgatgccaa caaacagcga catacgcgta tcttcattaa agtacatgcc gatcaaaacg
     2461 caaatcagga atgccattgt tacgtagttg gcccacggga acaggattga gcggaacgga
     2521 tggctggcaa tcgccgcttt atgtgcacgc cgaaaacgca gctggcttat caatatcaca
     2581 aaccatggca ccatccccgg aagcacgctg gcactgtaga cgtagacaaa cacacgctgc
     2641 ggattgggaa tgatgtagtt caggcatgag ccaattagca gaatagcaat agatactgcc
     2701 acacccgcaa ctggtacgcc gtgacgggaa actttcgcca tcgccgccgg taactgacgg
     2761 tttttcgcca gtgcgtagag catacgtccg caactgtaca tgccgctgtt acagccagag
     2821 agcgcagccg tcagcaccac aaagttgata atgcccgccg ctgcggtaat accgattttg
     2881 gcaaaagtca gtacgaacgg gctgccgttg ctgccaattt cattccacgg gaagatggtg
     2941 acgataacga aaatcgcgcc tacgtagaaa atcaggatcc gccacagcac tttgcctaca
     3001 gcactgcgca gcgtcacctg cggattcttc gcttcaccgg cagtaatgcc aatcagctcc
     3061 acgccctggt aggacgccac cacaatacac agcgcggtca ggaacccttt ccagccaccc
     3121 gcaaagaaac cgccatgctc tgtgagattg ctaaaaccaa tcgactgccc gccattgcca
     3181 aagccaaaga aaatcacgcc caggccaatg acaatcatca cgataatcgt ggtgactttg
     3241 atcatcgcga accagaactc gatttcgccg tacaaccgca ccgccgccag attcgccaac
     3301 gccaccagcg ccaccgcgat caatgcaggt atccactgcg ccatctccgg gaaccagaac
     3361 tggacataaa cgccaatggc ggtgatttct gagatcccca ccgccatcca cataaaccag
     3421 taagaccagg cggtgagata accaaaaaac gggctcatat aacgatgcgc ataaacggcg
     3481 aacgaaccgg taacaggttc aaggaacaac atttcgccca ttgaacgcat gatgaaaaag
     3541 acgaacagcc cggcgatgat ataggccaac aatacggatg gcccggccca tttcagggta
     3601 ctggcggccc ccataaacag gccgacgcca atagtgcccc ccagggcgat gagttcgata
     3661 tgtcgagctt ccagcccacg ctgtagctct ggtttgttat ctgccataaa tcctcgtgtt
     3721 gtgtttgcat gctttccggt gttaccggtt atcgttatgg gtacatcgag tgttgcgaat
     3781 gttttcgtaa ttcaggagaa atggcaaata aagcattaaa aatttgaatg ctttgtgtaa
     3841 taaaaaagca gacaggcgac ggagtgacca ctccgtcgct ttacaaagag aggaaaatca
     3901 gaggttgccg gtgtagtgcc agcgtaaata acgcagcaaa cgaagctgac gcttaatgcg
     3961 gctcggctgc gaaagcaggc ggtagagcca ctccagtccc agcgtttgcc agattttcgg
     4021 tgcgcgtttt acgtgaccgg tgaaaacatc gtaagtcccg ccaacgccca tatacagcgc
     4081 atctggatgc accagacggc agtcgcgcat gaagatctcc tgctttggcg atcccatcgc
     4141 aacggtgacg atttgcgctc cgctggcatg aatgcgttca aacagcgcct gacgctgctc
     4201 gggtttaaaa taaccatcct gactgccaac gatattcaca ttccattggt tgcgcagttt
     4261 agcttcagtt tgcgccagca cttcaggttt accgcccaca agaaataccg gcgtcccttc
     4321 tttgcctgcg cgcgccatca gctcttccca gagatcggca ccggcaacgc gggaaacctg
     4381 cgcctgcggg tactttttac gtactgaacg tacaacgctg atgccatccg catatttaaa
     4441 ttcggcagcg ttaattaact ccctgacctc ggcgttatct tcaatggtca gcattttttc
     4501 agcattaatg gcaaccagcg ttccctgctt aagctgcccg tcagcaaaca gataatcgag
     4561 ggcgtgctgc atatcacgcc aaccaatcaa ctgtaagcca cgcagcgtat aggtcggtgc
     4621 cgtggtgttg ttattcattg ttatccttca acctgcgtcc ggagcgatga ttttgtacgt
     4681 ttatgaatga gtccggcgct ttcaaaaagc cagtacagca gttttgcgat catcagacat
     4741 gcgccgaaga ccacgataaa aaagactacg cgtgagacaa acgaatccag cccttcacgc
     4801 gccagcacga tcatattgaa gatcgcccca aagcagaaac tgtgcaatat cgcagcctta
     4861 tagcgattag gctcgcggtt gcccagctca tacagccagt cgaaccattt gatgatcaga
     4921 ccaaccacga tcgccccgag cgggatgaac aacgcgccgc ccatcaccac cagtgagcct
     4981 ataagcgtag gcgagatcgc cagtccggag tggttattca gcacttccca ggtaaagtag
     5041 ttggctgagt tcagcaccat actcgggcga cccggccaca gccaggaagg gataaagaca
     5101 tagaaatcgc ggacaattgg agccaggccc tggaagtcga tgttgtcgta gttctgcaac
     5161 agcaacgcca gattctccca cggggagaag gtgtcgcgag tgagatagag aaacgtatag
     5221 aacgcttcat cgccgctcac attcattcca tagcgtttta gcgccagcca gaacatgcca
     5281 acaatcccca gcacgcccgc cgccgccagc atccacaacg aaatccagcc gcgaataatg
     5341 ccaataaaca ggaagatagc gaatgcgatg atgatattgg cgcgagtgcc gccgacaatc
     5401 atataagtca gcaagccaaa ggcgaccgtg ctgacgagga aaaacagcca cgctttgctg
     5461 tcctggcgca gaaagtagac caccagcatc gccgggatga aaaagtaaaa gaagcgtttt
     5521 aacgccacgc cggagacttc actggagaag atctgactgt aggagttaag ccggaacagc
     5581 aaaaagccgt tgtgcatgaa gaagatgccg acgcttacca gcgcgatacc catcaggatc
     5641 acccacgtaa gattggtctc cacgcggttc atggtaaaca gcggacggcg cggtacatca
     5701 gcaacgcgtt tgcgtaggcg ggttttgtag gtgacatagt aaaccgcgta gaagcagccc
     5761 gcagaaagca acgcctgcaa caagatttct ggcggcgcga caccaacatc aaagcgaaat
     5821 accagcacgc tggtcagcgg gaagccgaag aaaaaggtga gcaaaaacag caatgaaaag
     5881 aagacattga agttaaagcg cacacggcga aactcaaacc aggtcagcgt ggcaataaac
     5941 agcgtgcaga gcagccagac cacaaacagg ccactgaatt gcagcagact catgcgacct
     6001 ccccggcggc aatagccaac gcccgctgcc agccttgtag atagttaggg ctaaagaagg
     6061 caatggtgtt tttatccacc gacgccaact ggcgctgcgc ttcacgcaca atatcctcgt
     6121 tgagatcgtc ggtagtaaac agcaccggca aatgctgttc cgtcatatcc tgccagaacg
     6181 gattttcccg gttaagcaca caaggaatgc ccgcctgaat cagtaagcaa agcgtaccaa
     6241 tgccctgctg gcgggcaaaa ataaagtaac caagatcgca ctgacgaagt agcgccagat
     6301 aggcgtcaaa ttccagtttt tcgctcaaaa tttgcagatt ttcttcgctg aataactcca
     6361 gccccgcctg acgaacttcc tcaatgtacg cttcgttatt aggcggatat cccatcggca
     6421 ccaccacttt taccgtatcg ccaaattgct gatgaacggc gcgcaaggca gcaatatggt
     6481 cattgctgcg gtcaccggag ttccccacca gaatggtcat tttcccttca cgttgccgat
     6541 cattcgccat cgtattgaga gaagggtcca tccgcgtcgg gaagtacagc agttcgcccc
     6601 gcacctttgg atgcgttttg gcaaaaaagc tcaaatcacc gcgggtggca aatacacagc
     6661 cgactcgctt ttgcgccagg cgacgtagcg ggtaaaaaag cttatatctc aagccactgg
     6721 aaagctcata caggtctgcc ccccagatat gccagaaaaa ctggctgggc ttaatgccac
     6781 cactcagcag agccagccac agtgtgggat tgaactgacc gtggaaaaaa aatcgctgct
     6841 gacggttagc ttttgctttc gcgatgaccg cttccgccag cgattttttc ccagggaaaa
     6901 attgcacaga aagcgccgga cagctatcac ttaagccgtc gtccttgcca acaaccataa
     6961 actcgcgcgc gtgctcgctc gtcgcggcca gcgcgtcatt gaaaaaccgc aaaacggttc
     7021 ggttatggtg agggatatcc gatcccagta cgtgaatcag tacagtcatg cccgcctacg
     7081 ccagagtaaa aacacgccac aacaaagaga aaaatagacg atataagttg ccatatatgc
     7141 ctgcgccgcg cccagtgcac catgcgcagg gattagccag tgggcaaata ccatcaataa
     7201 agtgaactgg ctgacttccg ccagaatata aaaccgcagt gacgctttgg cgatcaccag
     7261 ataaccaaag acataagcgc ccacttttaa cacatcaccc actaactgcc aggcaaagag
     7321 atcgcgcata gcggtaaatt tattcgacaa cagcaaccag atagcaaaat cacgcagcaa
     7381 ccagacggta aaactcgccg ccgccactgc cggtaagacg aatttcagcg atttaaccac
     7441 ttcccgggtg atatcgcgct tttccgttag ccgcgacaac gtgggcagca aatatacgct
     7501 gaacgatgcc gtaataaatt gcaggtaggc atcggaaata ctgctcaccc cttgccagat
     7561 ccccacttca tcccagctat actgcgccgc cagcagttta cgcatcatga tgtaagcaac
     7621 aggcaaggtc accgacgtaa tcaacgccat gagcgtaaat ttgctcaact gccctgccag
     7681 accgttatcc cagctgggtt tcagatagct taacgggatg acaccacgtt tgatcaacat
     7741 gatggcggca ggaatcacca ccagcgcggg aatcagcgcc agacccagca acgccccttc
     7801 ataaccgccc aaacggtaac tgacgtagta cgcgagaacg ccaatcaagc tgccgacaat
     7861 cagcgataac gcattacctg cggcatcgcg aaagcctttc atcagcgcca gtaacaggtt
     7921 gccccaggcg atccccattt gcaccagcgc cactaaacgc accagcccct gatagtcggt
     7981 attaccaaac agtccctggc tgattggcgc agctgccagc acaaaaacca gcgccatcag
     8041 cgtagagaaa ccaagtacca tcgctgatga agtgccgacc acgcggcgca gctgttgcgg
     8101 attatcatgg tactgggcaa cgtatttggt tacaccgtta aagatgccag ctccggcaag
     8161 cacgccgagc acggtaatca actggcggaa atttgccgcc agcccaagcc ccgccggacc
     8221 aaatgacaca gccagcaact taccgaccag taacccggca ccaatcttga ccagtgtact
     8281 ggccgccgtc cacaaggacg cttttgccaa cgacatatca ggagaagtag ttcaacaaag
     8341 tcgcaattac cgtacgctga ttgacgggcg acaggttgta gaacagcggc aggcgcagca
     8401 ggcgctcgct ctctttggtg gtgtagcgat cttcaccgtg gaactcacca aagcgttccc
     8461 ccgcagggca accgtgcagc ggaatgtaat ggaacaccgc catgatttcc gcttctttca
     8521 gaaagttaat caacgcgctc cggtcatcaa tatcccgcag tttaatgtag aacatatgcg
     8581 cgttctgcac gcagccatcg ggaatcgacg gcagctcgat acgcccggct ttcgccagag
     8641 gcgctaacgc atcgtagtag ttttgccaca gcgccagacg ttgctggttg atacgatccg
     8701 ctgcttccag ttgcgcccac aggtacgcag cttgcagatc ggacatcaaa tagctggagc
     8761 caatatcgcg ccaggtatat ttatcgacct gaccacggaa gaactggctg cggttagtgc
     8821 ccttttcacg gatgatctcg gctcgttcga ttaacgcttt atcgttaatc agcgtcgcgc
     8881 cgccttcacc acccgccgta tagtttttgg tttcatggaa gctaaagcag ccaatatgac
     8941 caatggttcc cagtgcacgc cctttgtaag tggacatcac gccctgagcg gcatcttcta
     9001 ccacaaacag attatgcttt ttcgccaacg ccataatggt gtccatttcg caggccacac
     9061 ccgcgtaatg gaccggcacg ataacgcgcg ttttgtcggt gatcgccgct tcaatcagcg
     9121 tttcgtcgat gttcatggtg tccgggcgaa catccacaaa aacgattttt gcgccacgca
     9181 gcacaaaggc attggcggtg gagacaaagg tgtagctcgg catgatcact tcatcgccag
     9241 gctggatatc gagcagcagc gccgccatct ccagcgaagc ggtgcaggac ggcgtcagta
     9301 acactttggc gctgccaaaa cgttgctcca gccactgctg gcagcgacgg gtaaaaccgc
     9361 catcgccaca cagtttgccg ctacccattg ccgactgcat atagtcgagt tcggttccca
     9421 ccaccggcgg tgcgttaaat ggaatcatgt gatcacctgt ataaccagta cgcggtgctt
     9481 tctacattcg caccactttg tatgtatcgt ttaagcgcgg cggtgttgcc catttgggtc
     9541 gccacccgca aagttgtttt accgcgagca tacgcccagt ttagcgccgt ttgcatcagc
     9601 tcagcacctg caccgcgtcc agccagcagg ccaattcgcg catctgtcgc attgagttcc
     9661 cgtaaagaga cataaccgcg aataccgccg gacgccgcac gtaaaatcag acattgatga
     9721 tcaaaggtgc cgcgcacggc attttcaatc cactgtgcat aaaagcgtcc gctggcgtca
     9781 ggcgcatacc acggcgcacg aaaacggctt tgcgcaaatg cggcgctggc taactgacgt
     9841 aatgcgggaa tatcggtctc ttgtgccact acagcaccac tatcactgac attgttcacg
     9901 ggtagtgcca aatcaacttc accttctacc agggagaatc ccagctgttg cagggcatcc
     9961 agttcacccg tatttgatgc cgcaattttg gcctgcaccc gtgaccacgg cgctaacacg
    10021 tctggcgtca ggagctgtgc ttcagacgta atgcgcacga tggcgctgtt aacaccaaag
    10081 aaggcgtttt cccaggttag tggctcaata ctggcgcgga cgggcacgaa gtaactccag
    10141 cagatattgg ccgtagccag ttttcgctaa tgaactggca gcacgcttca caccctcgtc
    10201 atcgagccag ccgttacgcc aggcgatctc ttccaggcag gcaatcttaa agccctggcg
    10261 tttttctact gtctgcacaa aggtactggc ttcaatcagg ctgtcgtgag tgccagtgtc
    10321 cagccaggca aatccgcgcc cgagcagttc aacggtcagg ttgcccgcct cgaggtacat
    10381 ctggttgatg gaggtaatct ccagttcacc acgctccgac ggcttcacct gctttgcgta
    10441 ctccacgact ttactgtcgt agaaataaag cccggtcacc gcccagtttg acttcggctg
    10501 ttttggcttt tcttccagcg agatagcgcg gaaattgtcg tcaaactcca ccacgccaaa
    10561 gcgttccggg tccatcacct gatagccaaa taccgtcgcc ccttccgtgc gcgccgcaac
    10621 atgacgcagc ttcggactga agccctgacc gaagaagatg ttatcgccca gcaccagaca
    10681 agaaggttca ccgttgagga aggtttcacc gatgataaag gcctgcgcca gaccgtccgg
    10741 gctgggctgt tcggcgtatt ccagctggat accgaactca ctaccatcgc ccagcaggcg
    10801 ctggaaataa cctttatctt ccggcgtagt gatgatgaga atttcgcgga tatcggccaa
    10861 catcagcacc gacagcggat agtaaatcat tggcttatcg taaatcggca acagttgctt
    10921 cgatacgccg cgcgtaatcg gatgcaatcg ggtgccggaa ccgcccgcca ggataatacc
    10981 tttcatgttt gcctccgctg aaaattagcc tttcagacct aaacgctcgc cctgatagct
    11041 gccgtcctgc acctgcttcc accagctttc attagccaga taccactgca ccgttttacg
    11101 cattccactt tcaaaggttt cctgtggcag ccagccaagt tcacgggcaa ttttcgaagc
    11161 atcaatggca taacgcagat catgccccgg acggtcagcg acaaaggtga tcaagtcacg
    11221 atagtgcacc acgccgtgcg gcttgttcgg agccagttct tccagcagct cgcaaatggt
    11281 ttccacaaca tcgagattct tacgctcgtt gtgaccacca atattatagg tttcaccgac
    11341 tttcccggtg gtcgccacgc aatacagcgc gcgggcgtga tcttccacat acagccagtc
    11401 acggatttgc tgcccgttgc catataccgg cagcggttta cccgccagcg cgttgaggat
    11461 catcagcggg atcagttttt ccggaaagtg gtaaggaccg tagttattcg agcagttggt
    11521 gataagcgtc ggcagaccgt aggtccgcag ccaggcgcgc accagatggt cgctgctggc
    11581 ttttgacgcg gaataagggc tgctcggcgc atacggcgtg gtttcggtga agaaatcatc
    11641 cgtcgagtgc aggtcaccat atacttcgtc ggtggagata tgatgaaaac ggaacgctga
    11701 ttttttatct tccgtcagcg tattccagta agcccgcgcc gcttcaagca atgtataagt
    11761 cccgacaatg ttggtttcaa taaacgctgc cgggccgtca atagaacggt caacatggct
    11821 ttcggctgcc agatgcatga cacagtctgg ctgatgctca gtgaatacgc gtgccagttc
    11881 tgcccgatcg cagatatcaa ctttctcaaa ggcaaagcgc tcgctttgcg cgaccggtgc
    11941 cagcgacatc aggtttccgg cgtaggtcag cttatcgacc actaccaccg cgtcgctcgt
    12001 ttcgttgatg atataacgca ccagcgccga gccaataaac ccggcaccac ctgttatcag
    12061 aatttttctc atcagcgcca gactcctttg gcatcgacga catactgctg atggacattg
    12121 tcgccattga taactttaaa ctgactatga tcgaccagca tcaccagcac gtctgccgtt
    12181 gccagcgcct cgtcaagctg cgccagagta caaagcccgg tcagtttttt cggcaactgg
    12241 tggatgttag gctcaacaac cagagtttcg ccgctatgcc actgggcgat cagttcagcg
    12301 atttccatcg ccgggctttc gcgcaggtca tcaatattcg gtttaaacgc cagaccaaag
    12361 caggcgattt tcagttcact ggcgcgttta tcggtagccg ccaggcaatc agccaccgcc
    12421 gctttcacct gatcgataac ccagaacggt ttgtgatcgt tcacttcgcg cgcggtacgg
    12481 ataagccgcg cctgctgggg gttctgtgcc acgataaacc acggatcaac agcaatgcag
    12541 tgaccgccca cgccagggcc aggctgaaga atattaacgc gagggtgacg attcgccagg
    12601 cgaatcagtt cccagacgtt aatcccctga tcggcacaaa tcagcgacaa ttcattagca
    12661 aaagcgatat tcacatcgcg gaagctgttt tcggtgagct tacacatttc cgccgtccgc
    12721 gagttagtga cgacacactc accttcgagg aaaattttgt acagttcgct ggcgcgggtc
    12781 gaacaaaccg gcgtcatacc accaatcacg cgatcgtttt taatcagctc gaccataacc
    12841 tggcccggta acacgcgttc cgggcagtaa gcaatgttga cgtccgcctg ctcgcccacc
    12901 tgctgtggga aagtgagatc cggacgcatc tctgctaacc attctgccat cttctcggtt
    12961 gaccccaccg gcgaggtgga ttcaaggatc accagcgcgc cttttttcag cactggcgca
    13021 atggagcgag cagccgattc aacgtaggtc atatctggct catgatcgcc tttaaacggc
    13081 gtgggtacag caatcagcca ggcatccgct tcaactggcg tcgtgctcgc tcgtaaaaaa
    13141 ccgccttcta cggcagtttt tactacgctc gccaaatcag gctcgacgat atggatttcg
    13201 ccacgattga tggtatcaac cgcgtgttgg ttgatatcga caccaatcac ctgtttttgc
    13261 cgtgaggcaa acgctgctgc tgtaggcagc ccgatgtaac ccagtccgat aacagaaatg
    13321 gtcgcaaaac tcatagtgat atccgattat tttttaacgc ttccagaatg cgagagcatg
    13381 cctgaccatc accatacggg ttatgggcgc ggctcatagt ttgatattcg ttttcgtctt
    13441 ttaaaagacg cgtcacttcc tcgacaattc gctgcttatc cgtgcctacc agacgcaccg
    13501 tacccgccgt caccgcttcc ggacgctcag tggtatcgcg catcaccagc accggtttcc
    13561 ccagcgaagg cgcttcttcc tgaatgccgc ctgagtcggt caaaatcagc caggcgtggt
    13621 tcatcagcca gacaaacggt aaatactcct ggggatcgat cagaatgaca tttttcacat
    13681 gccccagaat gcgattgacc ggctctctga cgttcgggtt gagatgcacc ggatagacaa
    13741 tctggatgtc ctggtgcgtg gtggcaatgt ctgccaacgc gtggcagatt tcttcaaagc
    13801 cacgaccgaa actttcacgc ctgtgaccgg tcaccagaat catcttttta tcggggtcga
    13861 taaacgggta atttgccgcc agttctgaac gcagcgtatc gctgctcatc acctggtcac
    13921 gcacccataa cagtgcatca ataactgtat taccagtaat gaagattcgg ctatccgcaa
    13981 cgttttcacg cagcaagttt tgccgggaag tttcggttgg agagaagtga tacatcgcca
    14041 gatgcccggt caatgtacgg ttagcctctt ccggccacgg cgaataaaga tcgcccgtgc
    14101 gcagaccagc ctcaacgtga ccaacaggaa tacgctgata aaacgccgcc aggctggttg
    14161 ccagcgtcgt cgttgtgtcg ccgtgaacca gcacgacgtc tggtttgaac tcggcaagaa
    14221 taggttttag cccttccaga atccgacagg ttatctctgt caggccctgt cctggctgca
    14281 ttatgttgag atcgtagtca ggtacaatgg aaaagagttt cagcacctga tcgagcatct
    14341 cccgatgctg cgcagtgacg caaactttag cctcaaaaaa aggatctttt gccaacgcat
    14401 gcaccaacgg cgccatcttg atggcttccg ggcgcgtacc aaatacagtc agtactttca
    14461 catcgattct cttcgaataa gcggcgagcg cctttgcgct caccgcagca gtgttgctat
    14521 ttcgagcaac ggcgggttaa tgcgacacca gccccgatca gccccccgac aatgccccac
    14581 ataatcatca ggaaggcacg acgtgggcta tcgcgtttta ccggttcttc cggcgtacgc
    14641 aaatagcgat aggtctgaaa acgcggatcc agggttggac caacattcag ggtgtttaac
    14701 atggcccgat tctgatcata gtcgagatca aaggccggac cgacggcctg taaattttcc
    14761 agtcgagcct ggagcattgg acgcccaagc aggaacattt ctgaatcagg taattcctcg
    14821 gcaggcacat ctgtcgcact gcgcgaaata ttatgctgct cagcaatttt cagcgcctgt
    14881 tcaatactgt tcatccggcg gtcgtagatg gctttcgcca cctcttcctg acgcttcacc
    14941 tgtgctttca tctggatggt acgcgccgcc catgcgcctt tcagctcatc attcagatgg
    15001 ctggctgcac gctggctggc aaaagcaaca tactgacgta acaggttatt agcgtcaggc
    15061 gcggtttcag caataagctt cacgctgtca ttgaccgcgc gggtaaagtc tccggggata
    15121 aactggatgt tgttaatcat ttcatccagc aacgccgcat cggctttgct gttgcccacc
    15181 atccgctgtt tgtaatagtc ggtttgcagc cagaactctc tgcgggtatc ccacgaggcc
    15241 agttgcataa caaactcttt ataggcttcg tccatgaccg atggttggtc ggcagaagcc
    15301 atgtttgaac ggacatccag gttacgcaaa aattgctgct gcgagtaata tccccccagc
    15361 atattcaccg ttggacgatc ggtaatcgcc gtcgagctcc actcctgacg agcaaaaaaa
    15421 gtatacgcca gcgcgattaa cgcaaatgcc agccccatgc caataatcca tagcttccca
    15481 gcccacaagg tacgaaacaa cccacgaata tccagttcat tttcagcgtc ttcggccggt
    15541 ttcccaggca ttggttgtgt catcacatcc tcatttattt ggttaaattg gggctgccac
    15601 cacgatttct acgcagtctg cgttttacgc gcttaataaa gcgagcaact ttccaggcac
    15661 gcttaatgca atatccatag aggaagaatg ctagcaaaaa gagcaccagc atgacccact
    15721 ccgggacaaa atgagaatat tctgccagca cgccaatgga agcgagcagt gccgcagcaa
    15781 gggtaatcag cacaaacgcc tgacgggaag taaacccggc acgcatgatc aaatggtgaa
    15841 tatgctgacg gtcaggagag aatgggctca tgcctttacg caggcgacgg tacataatcg
    15901 ccaccatatc cattagcgga atggcgatta tccacaaagc ggtaaccggg ctgatgggat
    15961 gggttttgcc ctgggtcgtt tcgagcagga tccagataac ggtaaaacca atcagcgtac
    16021 tgcccgcatc acccataaag actttgtagc ggcgacccag gataccaagg ttaagcatga
    16081 tgtatggcag gatggcggcg atcatcgcaa agcaccagat tgcgaggctg gtttgcccgt
    16141 cgaaccacaa aatcataccg attgctgcaa acgagacgca ggacaacccg cccagcaagc
    16201 catcaatgcc atcaaccatg ttgaacgcat taatggccgc ccagacggca aatagcgtca
    16261 ggaagtaacc aaacggtccg agcaccatct cccaggagcc aaagatataa cccaggctac
    16321 tgagataaag tttgccgaac accatcataa caatgccaac agcggcctgt atggtggcac
    16381 ggatttttac gctgatatca aaacggtcat ccagcgcgcc aatgaaaaca agcacaccgg
    16441 cacaagcgag atagagagat gcatgcggaa tatagtaatc gacaattccg aacgtgaagc
    16501 aaatccctgc gtaaaccgaa atccccccaa cgagaggtat caatccctgg tgacgtttgc
    16561 ggaagtttgg tttatccact aaaccgactt tttttgccac cttacgggca aaaaacagaa
    16621 acagtgtcgt gaataaaaaa atactgatga gatcagtact cactgtcagt aaattcacaa
    16681 tgcatgctct cagagaaaat tattagcaga agtataacca cgaagacctt tattcagaag
    16741 ggaaagtccg ttccgaaccc accagtcctg tgcaaaaatt aaccatttga tatgcaaatt
    16801 gcataacacg ccagcaaatt ccaggaattt tcctaacatc gccagcgcgg cattaagatt
    16861 acagatataa aagcaaaacg ccacgtaaac acgtggcgtt tttggcataa gacaaattta
    16921 tgagcgtttc atcatttcga agaaatcgtc attggtcttg gtcatggcca gtttattaat
    16981 gaggaattcc attgcatcga tttcgcccat cgggtgaatg attttgcgca ggatccacat
    17041 tttctgcagt tcttcctgag tagtgagcag ttcttcttta cgggtaccgg aacggttgta
    17101 gtcgatagcc gggaagacgc gtttttcagc gatcttacga gagaggtgca gttccatgtt
    17161 gcctgtacct ttaaactctt cgtagataac ttcgtccatt ttagaaccgg tatcgataag
    17221 cgccgtcgcg ataatagtca ggctgccgcc ctcttccacg ttacgcgccg caccaaagaa
    17281 gcgtttcgga cgatgcaggg cgttggcatc cacaccaccg gtcaacactt tacctgacgc
    17341 cggaacaacg gtgttgtaag cgcgcgccag acgagtgatg gagtcgagca gaatgataac
    17401 gtctttcttg tgctcaacca ggcgtttggc cttctcgatc accatttccg caacctgaac
    17461 gtggcgagat gcgggttcgt caaaggtaga agcaacaact tcacctttta ccagacgctg
    17521 catctcggtt acttcttccg gacgttcgtc gatcagcaga accatcagca cacaatccgg
    17581 gtggttgtaa gcaatgctct gggcaatgtt ctgcagcagc atggttttac cggctttcgg
    17641 cggtgccaca atcagaccac gctgaccacg accgataggt gatgccagat ccagtacgcg
    17701 agcagttaaa tcttcagtag aaccgttacc acgttccata cgcagacgag aatttgcgtg
    17761 cagcggggtt aagttctcaa agaggatttt gttgcgggcg ttttcaggtt tgtcgaagtt
    17821 aacttcgtta actttcagca gcgcaaaata gcgttcacct tctttcggcg ggcgaatctt
    17881 accagagatg gtatcaccag tgcggaggtt gaaacggcgg atttggctag gggaaacgta
    17941 gatgtcatca ggaccggcga ggtaggagct gtctgcggaa cggaggaaac caaatccatc
    18001 ctgcaatatc tccagtacgc catcaccaaa gatatcttcg ccactctttg cgtgctgctt
    18061 caggatggca aaaataatgt cctgcttacg catgcgagcc aggttttcca gccccatatt
    18121 ttcgccgaga gtgatcagct cagaaaccgg cgtattcttt aattcggtaa gattcatagt
    18181 ggtgtgagtt cttaaacttg gggtaaatct cgaacttaat gttgtgaatg gtatggcagg
    18241 atcatccatg cctgtttacg gtcatcgact catgtctttt cgctgcctgg tcacaggaga
    18301 gtacgcagaa ctgaaacgac aagacggatt gagtgacgag ccagaaatct gttcacttcg
    18361 catttaagat aaaacgggaa gcgttggaaa ttacaagatt caaacttaat aaggtatgtt
    18421 taatacgaag tcaacactaa gttagcatga ctcacgccgg gcgtccagtt tttagcgacg
    18481 gggcacccga acatgaaatt cccttacgcc aggttagcgt cgaggaactc tttcaactga
    18541 cctttagaca gtgcgcccac tttggttgcc gccacttcac cgtttttgaa cagcagcaga
    18601 gtcgggatac cacggatgcc atatttcggc gcagtgccag ggttttgatc gatgttcagt
    18661 tttgcaacgg tcagtttgcc ctgatattcg tcagcgattt catccagaat cggggcgatc
    18721 attttgcacg gaccgcacca ctctgcccag aaatcgacga ggatcgcccc gtccgctttg
    18781 agtacatccg tgtcaaaact gtcgtcagtc aggtgaataa ttttatcgct catatataac
    18841 tccacaggaa taagcctggc gtgttggttt cgttgttggt gtaacattaa ccaactaaag
    18901 gttgacttta tttcaccgga tacgctttcg taaagcaata gtaagctgat attctaccac
    18961 actatgagca aaacacattt aacagaacag aagttttccg acttcgccct gcatccgaag
    19021 gttgtagaag cccttgaaaa aaaagggttt cataactgta cgcccattca ggcactggcc
    19081 cttccgctga cgctggcggg tcgtgacgta gccgggcagg cgcaaaccgg taccgggaaa
    19141 acgatggcgt ttcttacgtc aacgtttcat tatcttctct ctcatcctgc gattgccgat
    19201 cgcaaggtga atcaaccgcg tgccttaatt atggcaccga cgcgtgaact tgccgtgcag
    19261 atccatgccg acgcagaacc gctggcggaa gctactggcc tgaagctggg tctggcttac
    19321 ggtggtgatg gctacgacaa acagctgaaa gtgctggaaa gcggcgttga cattctgatt
    19381 ggcaccacgg gtcgtttaat tgactacgcc aagcagaacc acattaacct cggtgccatt
    19441 caggtagtgg ttctggatga agccgatcgc atgtacgatc tgggctttat taaagatatc
    19501 cgctggctgt tccgccgtat gccgcctgca aaccagcgcc tcaacatgct gttctccgcc
    19561 acgctttcgt accgggtacg tgaactggcg ttcgagcaga tgaacaatgc cgaatatatt
    19621 gaagtggaac cggaacagaa aacgggccac cgtattaaag aagagctttt ctacccttct
    19681 aacgaagaaa aaatgcgttt gctgcaaacg ctgatcgaag aagagtggcc agacagagcg
    19741 attattttcg ccaataccaa acaccgttgt gaagagatct ggggccacct ggcggcagat
    19801 ggtcatcgtg tcggtttgtt gacaggcgat gtcgcgcaga aaaaacgtct gcgtattctt
    19861 gatgaattta cccgtggcga tctggatatt ctggttgcca ccgacgttgc cgcgcgtggt
    19921 ttgcatattc cggcagtgac gcacgtcttt aactacgatt tacccgatga ctgtgaagat
    19981 tacgttcacc gtattggtcg tacaggtcgc gcaggcgcaa gcggtcactc tatcagcctg
    20041 gcgtgtgaag agtatgcatt gaatttgcct gctattgaaa cctatattgg tcactcaatt
    20101 ccggtaagca aatacaatcc ggacgcattg atgaccgatc tgccaaaacc gctgcgcctc
    20161 acgcgcccac gtacaggcaa tggtccgcgt cgtactggcg ctccgcgtaa tcgtcgtcgt
    20221 tcaggttaaa taaaatgtca tactcaaaat cattcaaact gtatcaagat ggcaagagaa
    20281 tgaatcccga tgagcatact gtagtaagtg attcaggtga aagaatgcag ccaacacaga
    20341 gacagattga aggatgaaga gtatgggttc cacctcgtcg ctgtatgcag ccattgatct
    20401 cggttcgaat agttttcata tgctggttgt gcgcgaggtg gctggaagca tccagacgct
    20461 gacgcgaata aaacgcaaag tgcgtctggc tgctggcctg aatagcgaaa atgccctgtc
    20521 taatgaagca atggagcgcg gttggcaatg tctgcgcctg tttgctgaac gtctgcaaga
    20581 tatccctccc tcgcaaattc gcgttgtcgc tacggcgaca ttacgtcttg ccgtcaatgc
    20641 gggtgatttt attgccaatg cgcaggaaat cctcggttgt ccggtacagg tgatcagcgg
    20701 tgaagaggaa gcacgtctga tttatcaggg cgttgctcac acaactggcg gtgccgatca
    20761 gcgcctggtg gtggatatag gcggtgccag tactgaactg gtaaccggca cgggtgcaca
    20821 aaccacctcg ttgttcagcc tgtcgatggg ctgcgtcacc tggctggaac gctattttgc
    20881 cgatcgtaat ctggggcagg aaaattttga tgctgcagaa aaagcggcac gcgaagtgtt
    20941 acgtccggtt gccgatgaat tacggtatca cggctggaaa gtgtgcgttg gtgcttccgg
    21001 caccgtgcag gcgttacagg aaatcatgat ggcacagggg atggatgaac gcattaccct
    21061 ggaaaagttg cagcaattga aacagcgagc cattcattgc ggtcggctgg aggaactgga
    21121 gattgacggg ctgacgctgg aacgtgcgtt agtgttcccg agtggtctgg cgatcctgat
    21181 cgccattttt accgaactga atattcagtg tatgaccctg gcgggcggtg cgctgcgtga
    21241 aggcctggtc tacggcatgt tgcatctggc cgtcgagcag gatattcgca gccgtacgct
    21301 gcgtaatatt cagcgccgct ttatgatcga tattgatcag gcgcagcgcg tagccaaagt
    21361 tgcggctaac ttcttcgatc aggtggaaaa ggaatggcat cttgaagcaa taagccgcga
    21421 tttgctcatc agcgcctgtc agcttcatga aatcggcctg agcgttgact ttaaacaagc
    21481 gccgcaacac gctgcttatc tggtgcgtaa tctggatctt cccggtttta cccccgcaca
    21541 gaaaaaattg ctggcgacgc tactgctcaa ccagactaat ccggtcgatc tctcatcgct
    21601 gcatcagcaa aatgccgtac cgccgcgcgt cgcagaacaa ctctgccgtt tactccgcct
    21661 ggccatcatt tttgccagcc gtcgccgtga cgatctcgtg ccagagatga cattacaggc
    21721 taaccatgaa ctgttgacct tgacgcttcc gcaaggttgg ctaacccaac atccgctggg
    21781 taaagagatt attgctcagg aaagccagtg gcagagctat gtccactggc cgctggaagt
    21841 gcattaatga gtaagtgccg gatgcgatgc tgacgcatct tttccggcct tgattatttc
    21901 cctcgttttg ccgccatcat cgctttcaga ttcgccagat ggctttgccc tttctgcatc
    21961 cgttcttcgg cgctgaccac tttgcgctcc tgttcccaaa tcagatcatc ctgcggcagc
    22021 tccagcaaaa agcggctcgg ctccgggcgc accagctcgc cgtactgacg gcgttcttta
    22081 cacagcgtaa aggtcaattc cttctgggcg cgggtaatgc cgacataggc cagccgccgc
    22141 tcctcatcga tattatcttc atcgatgctg ctctggtgcg gcaaaaaccc ttcttccata
    22201 ccgaccatgt agacataagg aaactccagc ccttttgacg cgtgcagagt catcagttgc
    22261 acctgatcca gctcttcttc actctcacca cgctccatca tgtcgcgcaa agtaaagcgc
    22321 gtcaccacct gggtgagcgt catcggctca tccagttcgc tgccttccag catctccgtc
    22381 atccagctaa acagttggtt gacgttcttc atgcgcattt cggcggcttt cgggctgggc
    22441 gatgtttcgt acagccagga ttcataatcc atgccgtgga tcagatcgcg caccgcggca
    22501 atcggctccc gctccgccag acgctggatt tctgccaacc agtgagtaaa gcgggtcaat
    22561 gcttcataac cacgtccgct aagcgtctga ctcaggccca tatcaaagct ggcggtaaac
    22621 atgcttttat tgcgcgtcat cgcccactca cccagctttt tcagcgtcgc cgggccaatc
    22681 tctctcttcg gcgtgttaac gatacgcaga aatgcgctgt catcgtccgg gttagtcagc
    22741 acgcgcagat aagccagcaa gtccttgatt tcaggacgag agaaaaacga cgtaccacca
    22801 gaaattttgt acgggatgcg gttttgcatc aggaattttt caaacactcg cgactgatgg
    22861 ttaccgcgat aaagaatagc gtaatctttg tactgcgttt tattgacgaa gtgatgcgca
    22921 atcagttcac cagtgacgcg ctcggcttca tgttcttcgt tattcgcgct taaaacttta
    22981 agctctgtgc cgtaacccag ttcggagaac agacgcttct caaagacgtg cgggttattg
    23041 gcgatcagga tgttcgccgc tttcaaaata cgtccggaag agcggtagtt ctgctcaagc
    23101 ttaatcacct tcagcgccgg aaaatcctga ctcagcagca ccaggttttg tggacgtgcg
    23161 ccgcgccagg agtagatcga ctggtcgtca tcgcccacca cggtaaagcg cgcgcggctg
    23221 cccaccagca gtttcaccag ttcatactgg ctggtgttgg tgtcctgata ctcatccacc
    23281 agcagatagc gaattttgtt ctgccagcgc tcgcggactt cttcattgcg ttgcagcagc
    23341 aacgtcggca ataaaatcag atcatcgaag tcgagaacat tgcacgcttt caggtgtgca
    23401 tcatacagcc cataacaatg ggcaaaaata cggtcccgct cgccaatcgc actggctgcc
    23461 gcctgggacg gtgttttgag atcgttcttc cagttagaga tggtcgaaat caactgttgc
    23521 aggagaactt tgtcatcttc aatcagccct tcggtcaact ctttgagcaa cgccagctga
    23581 tcggtatcgt caaacagcga aaagttcgcc ttcatcccca gcgccgcata ctcacgcttg
    23641 atgatatcca gccccagcgt gtggaaggtt gagatcatca gcccacgcgc ctctttgcga
    23701 cccagcgtct gtccgacacg ctctttcatc tcgcgcgccg ccttattggt aaaggtcacc
    23761 gccgcgatat gtcgcgcctg ataaccgcaa ccgcggatca gatgggcgat tttattggtg
    23821 atcacacgag ttttaccgga acccgcgccc gccagcacca ggcaggggcc ggtaacgaat
    23881 tcgacagctt gttgttggcc ggggtttaga cgcataggtg tattgctcaa tcttcgaacg
    23941 gggggaggat tgtagcagaa tcgcatttga atgtgaccgc gtgtaagccg ctaaccgatt
    24001 ggctgcggat gtttaaatca gagagggaag accgaaaccg gagttcatcg tgaatccatt
    24061 agacttagaa aatatcgggt cgtaacgttg tgttattact ttttcagagt ttactaagga
    24121 gaggtgtact cgtggcgatt aatttcagtc ctaaggttgg tgaaatactg gattgcaact
    24181 ttggtaacta tcctgttagc cagaatggac agttcagcac cacgtattac gatggccgca
    24241 ttcctcctga aatgataaaa aacaggttag t
//
LOCUS       DAZPAC010000048        24154 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZPAC010000048 DAZPAC010000000
VERSION     DAZPAC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24154
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.048"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            520..759
                     /locus_tag="ACHUPM_001585"
     CDS             520..759
                     /locus_tag="ACHUPM_001585"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR0869400.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(947..1924)
                     /gene="intI2"
                     /locus_tag="ACHUPM_001586"
                     /pseudo
     CDS             complement(947..1924)
                     /gene="intI2"
                     /locus_tag="ACHUPM_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2256..2729
                     /gene="dfrA1"
                     /locus_tag="ACHUPM_001587"
     CDS             2256..2729
                     /gene="dfrA1"
                     /locus_tag="ACHUPM_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR0869401.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2824..3348
                     /gene="sat2"
                     /locus_tag="ACHUPM_001588"
     CDS             2824..3348
                     /gene="sat2"
                     /locus_tag="ACHUPM_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0869402.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3417..3914
                     /locus_tag="ACHUPM_001589"
     CDS             3417..3914
                     /locus_tag="ACHUPM_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869403.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            3975..4346
                     /locus_tag="ACHUPM_001590"
     CDS             3975..4346
                     /locus_tag="ACHUPM_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869404.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            4756..5133
                     /locus_tag="ACHUPM_001591"
     CDS             4756..5133
                     /locus_tag="ACHUPM_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869405.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            5156..5308
                     /locus_tag="ACHUPM_001592"
                     /pseudo
     CDS             5156..5308
                     /locus_tag="ACHUPM_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5364..6980)
                     /locus_tag="ACHUPM_001593"
     CDS             complement(5364..6980)
                     /locus_tag="ACHUPM_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR0869406.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(6981..8507)
                     /locus_tag="ACHUPM_001594"
     CDS             complement(6981..8507)
                     /locus_tag="ACHUPM_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR0869407.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(8510..10177)
                     /locus_tag="ACHUPM_001595"
     CDS             complement(8510..10177)
                     /locus_tag="ACHUPM_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0869408.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(10174..12282)
                     /locus_tag="ACHUPM_001596"
     CDS             complement(10174..12282)
                     /locus_tag="ACHUPM_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0869409.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(12269..13090)
                     /locus_tag="ACHUPM_001597"
     CDS             complement(12269..13090)
                     /locus_tag="ACHUPM_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR0869410.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(13250..15079)
                     /gene="glmS"
                     /locus_tag="ACHUPM_001598"
     CDS             complement(13250..15079)
                     /gene="glmS"
                     /locus_tag="ACHUPM_001598"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR0869411.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(15241..16611)
                     /gene="glmU"
                     /locus_tag="ACHUPM_001599"
     CDS             complement(15241..16611)
                     /gene="glmU"
                     /locus_tag="ACHUPM_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR0869412.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(16962..17381)
                     /locus_tag="ACHUPM_001600"
     CDS             complement(16962..17381)
                     /locus_tag="ACHUPM_001600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR0869413.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(17402..18784)
                     /gene="atpD"
                     /locus_tag="ACHUPM_001601"
     CDS             complement(17402..18784)
                     /gene="atpD"
                     /locus_tag="ACHUPM_001601"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR0869414.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(18811..19674)
                     /gene="atpG"
                     /locus_tag="ACHUPM_001602"
     CDS             complement(18811..19674)
                     /gene="atpG"
                     /locus_tag="ACHUPM_001602"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR0869415.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(19725..21266)
                     /gene="atpA"
                     /locus_tag="ACHUPM_001603"
     CDS             complement(19725..21266)
                     /gene="atpA"
                     /locus_tag="ACHUPM_001603"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR0869416.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(21279..21812)
                     /gene="atpH"
                     /locus_tag="ACHUPM_001604"
     CDS             complement(21279..21812)
                     /gene="atpH"
                     /locus_tag="ACHUPM_001604"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR0869417.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(21827..22297)
                     /gene="atpF"
                     /locus_tag="ACHUPM_001605"
     CDS             complement(21827..22297)
                     /gene="atpF"
                     /locus_tag="ACHUPM_001605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR0869418.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(22359..22598)
                     /gene="atpE"
                     /locus_tag="ACHUPM_001606"
     CDS             complement(22359..22598)
                     /gene="atpE"
                     /locus_tag="ACHUPM_001606"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR0869419.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(22645..23460)
                     /gene="atpB"
                     /locus_tag="ACHUPM_001607"
     CDS             complement(22645..23460)
                     /gene="atpB"
                     /locus_tag="ACHUPM_001607"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR0869420.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(23469..23849)
                     /gene="atpI"
                     /locus_tag="ACHUPM_001608"
     CDS             complement(23469..23849)
                     /gene="atpI"
                     /locus_tag="ACHUPM_001608"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR0869421.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         6281 a         6105 c         5430 g         6338 t
ORIGIN      
        1 tcggcagccc tgtgggcgga caaaatagtt gggaactggg aggggtggaa atggagtttt
       61 taaggattat ttagggaaga gtgacaaaat agatgggaac tgggtgtagc gtcgtaagct
      121 aatacgaaaa ttaaaaatga caaaatagtt tggaactaga tttcacttat ctggttaact
      181 aatctagcta acctgccgct cagcttagta cgatcctata tccaactgat cataatcctt
      241 tcatcagtat cccaaattaa atccccctcc tagactgcct caccaacact ttacagtgat
      301 cccgtatttc cggacaggtg attgattttt ttcgcttgta caggtgttaa gccacagtta
      361 tcttgatgtg gacattatgc attgtaatag cccattaaca tgcgctaaga gcataaatta
      421 atatacagta acaagcatta cgaggtgcta atgtttggaa agcagcctct tccaactaca
      481 ataataagtt aactatcctc cacccacaaa taattacaaa tgaccccttt ccgctataac
      541 tccgatctca ccagcggctc gctacaaacc cgagagtgtc gcattattac tggcttgtta
      601 ctccaagagc ttgatgaagc tgcctgggat aaagccatgt ataaggaaaa tgtgcttcag
      661 aaacgcacgc aatctacggt aagacgtatt tcttcagcgc ttagaaaacg cctagaacac
      721 ctaagctctg acttttgggc ttttgcgttt ttatgttagg cattgtctgg caactggagg
      781 aagtgtaatg accatgaatg gtcactgtga ttgacagtat cttgcttaaa cttatctgaa
      841 caatggtaga actggctcat atttatcccc tctatgattg tttatctttt tcaccataaa
      901 aagtatcagg ggctactcag ggctacataa agatatttat ctacttttac tgattgataa
      961 gtagcatcag tccatccgca ggactggtgg tgccggcaaa atgctgaccc aacacatgcg
     1021 tatagatttg cgtggtctta acatcgttat gccctaagag ttcttgcaca gtgcgaatat
     1081 cacgccccgc ttgtaataga tgcgtagcaa acgagtgacg aaatgtatga caagtgacac
     1141 gcttgctaac gatgcctgct ttttgtacgg ctgccttcaa tgcctttcgc gcaacggagt
     1201 catgcagatg atggcggcat aatttgccgt tatacgggtg gttgcagagc gtgctggagg
     1261 gaaagacaaa catccacgcc gcttgtcgat aagcagaagg gtatttgtga tctaaagcaa
     1321 aaggcagcga tggccctacg ccttgtaagt tgtcgtcttg ctgaataagc cgcgcttgct
     1381 caatgagtta ttttattgct gggattaggc gcgtgggcag taggctgttt ctgcttttcc
     1441 cacccttacc gtcatgcaca gtgatgcagc cattatcaaa atcaaaatct ttaacccgca
     1501 aacgcaagca ttcattaatg cgcaaacctg caccatacag cagcgtaaaa ataacttggt
     1561 tgcgagtatc cataacctgc aaaatgcgtt gcacttcatt tgcagagata acagagggta
     1621 gccgtctagg cttgcttgca gggatataat caatatcgcc caacggctgt tgtaaaaacc
     1681 tgttgtacaa aaaagctagg gcatttaaag cgattttctg cgtgtttatg gctacatgtc
     1741 tgctgtttgc taagctggat aaaaacagcc tgacctcttc actgcccatg gtctgaggat
     1801 gacgtttttt gtgaaacaga ataaaacgct taatccagtg caggtaagtt ttttcagttt
     1861 tcagcgcata acctttttgt cgcatatccg tgcgtataga atttaaaaat ggactgttag
     1921 acataaaacg ctccttgtct tgcaactgtc tgcctataca gcctattcta gctgggattt
     1981 aaaaaagtgc ctgtttttta cgcctagaga tgcttgttta ccggtagagt tttaatttaa
     2041 tgctaaataa attaaaatgt tatgagttct ttgggtgaga taatgtgcat cgtgcaagca
     2101 ggatagacgg catgcacgat ttgtaataac agagtgtctt gtatttttaa agaaagtcta
     2161 tttaatacaa gtgattatat taattaacgg taagcatcag cgggtgacaa aacgagcatg
     2221 cttactaata aaatgttaac ctctgaggaa gaattgtgaa actatcacta atggtagcta
     2281 tatcgaagaa tggagttatc gggaatggcc ctgatattcc atggagtgcc aaaggtgaac
     2341 agctcctgtt taaagctatt acctataacc aatggctgtt ggttggacgc aagacttttg
     2401 aatcaatggg agcattaccc aaccgaaagt atgcggtcgt aacacgttca agttttacat
     2461 ctgacaatga gaacgtattg atctttccat caattaaaga tgctttaacc aacctaaaga
     2521 aaataacgga tcatgtcatt gtttcaggtg gtggggagat atacaaaagc ctgatcgatc
     2581 aagtagatac actacatata tctagaatag acatcgagcc ggaaggtgat gtttactttc
     2641 ctgaaatccc cagcaatttt aggccagttt ttacccaaga cttcgcctct aacataaatt
     2701 atagttacca aatctggcaa aagggttaac aagtggcagc aacggattcg caaacctgtc
     2761 acgccttttg taccaaaagc cgcgccaggt ttgcgatccg ctgtgccagg cgttaggcgt
     2821 catatgaaga tttcggtgat ccctgagcag gtggcggaaa cattggatgc tgagaaccat
     2881 ttcattgttc gtgaagtgtt cgatgtgcac ctatccgacc aaggctttga actatctacc
     2941 agaagtgtga gcccctaccg gaaggattac atctcggatg atgactctga tgaagactct
     3001 gcttgctatg gcgcattcat cgaccaagag cttgtcggga agattgaact caactcaaca
     3061 tggaacgatc tagcctctat cgaacacatt gttgtgtcgc acacgcaccg aggcaaagga
     3121 gtcgcgcaca gtctcatcga atttgcgaaa aagtgggcac taagcagaca gctccttggc
     3181 atacgattag agacacaaac gaacaatgta cctgcctgca atttgtacgc aaaatgtggc
     3241 tttactctcg gcggcattga cctgttcacg tataaaacta gacctcaagt ctcgaacgaa
     3301 acagcgatgt actggtactg gttctcggga gcacaggatg acgcctaaca attcattcaa
     3361 gccgacaccg cttcgcggcg cggcttaatt caggagttag agagctgggg aagactatgc
     3421 gcgatctgtt gaaggtggtt ctaagcctcg tacttgcgat ggcatcgggg caggcacttg
     3481 ctgacctgcc aattgtttta gtggatgaag ctcgtcttcc ctatgactac tccccatcca
     3541 actacgacat ttctccaagc aactacgaca actccataag caattacgac aatagtccat
     3601 caaattacga caactctgag agcaactacg ataatagttc atccaattac gacaatagtc
     3661 gcaacggaaa tcgtaggctt atatatagcg caaatgggtc tcgcactttc gccggctact
     3721 acgtcattgc caacaatggg acaacgaact tcttttccac atctggcaaa aggatgttct
     3781 acaccccaaa aggggggcgc ggcgtctatg gcggcaaaga tgggagcttc tgcggggcat
     3841 tggtcgtcat aaatggccaa ttttcgcttg ccctgacaga taacggcctg aagatcatgt
     3901 atctaagcaa ctagcctgct ctctaataaa atgttaggcc tcaacatcta gtcgcaagct
     3961 gaggggaacc actagtgtca tacgaacctc caagagacgg ttacacaaac gggtacattg
     4021 ttgatgtcat gtatgacaat cgcccaagta agtatccagc tgtgttcaga acgtacgtcc
     4081 gaattccaga ctatgtcgaa ctagataacg cggaggagct ggccgacgca atcattcgag
     4141 aggttgagtt aaaggaggca tggctcacaa gggtcttggg gaactcagcc gatcctgacg
     4201 cttctgtggg caagtgggac cggcgcctag cgcagtgcgg tggtattgat gagatgtcaa
     4261 gtggggtgca ggcaacatac ttcagtccaa acggtcagct ggtcggctta gtggcataca
     4321 ttgatgccga atggatggat gaatagctct gacgttacga ttcaggtgag acaaccagat
     4381 gggcattgcg tacaagctca gttgccaccg atgtggctac gagtctgatc ttctttacct
     4441 aggccaaggt atggcgatgc ttcctgagca aatagtcgga acgtgcacat gctgcgacaa
     4501 tctaacgacg atttctataa atgaaagcta cggagtatgc tcactttgtg gggctaagga
     4561 tcgtgtgata tttacaagct atcagcatga ggcaaggcca agaaatccct gctatctaga
     4621 gagaagtttc aaatatcaat gcccaagatg ccatgccttc agtatggagc ctccggatct
     4681 accggagatg ctttgggact aatgctccgc tttgcctcgg gtatccgttc gacctatgtt
     4741 taagaggaaa cgcacgtgtc tgccgccaaa gctcttgcct atgcactttg tgacaagttg
     4801 gaaagcaaaa aagttgatga agtcgtacga aatcttgttt ctagcggctg gaatattagg
     4861 gaagtggcct gggaagcttc gtcctttgat catgaaatgc cctactttga cacggggcgg
     4921 gacttcgata ttggatttcc tgacacgctg tcatccaaga ttgagttttt gaccgcgaat
     4981 ggatcaatcg tccaagcacg tgctcgcctg cacttcaagc aggctctaat ttttgccaag
     5041 gcgtccaaac atgtcgccgc gctcaagaac tcattggatt actactatgg atcgtctgta
     5101 atcccccctc aaaaccacgg aggctatttg tagaaaattc cattaatatg aactcaaggt
     5161 gttccctacg gacgaatcag cgatgaaggt ggtgtacctg gctatccagg cggcgaccaa
     5221 gaaatggacg atgccgatcc gcaactggaa gccggccatg aaccgtttta tgatcgaatt
     5281 tggtgaccgt ttaaatggcc acctttaact gaccggcaaa agcacttaca caaaagggtt
     5341 tacaggctct ttcagtcatt cctttaatgc gtaaattgct ctctaaccac tctctcagcc
     5401 cacccttcca tatcctctct cgatacttgc agctccccca acccctttgg atgattcacc
     5461 cctctatgcc cgtcttgtcc atataattga tcaaacaaac tatttggcca attcaatgag
     5521 ctcttcacca ctccacgccg tatcttttca aaatcattcc gccatgtttc gctatcaacg
     5581 ccactcaaca ctttggtaga aagcattttc accccatccg atgcatccac ctctagcaat
     5641 acaaattcac tgccattacg ttgcacccca acggctttaa tcactcttgg tgagccatct
     5701 ttacacagat gtaatcggct acgcccaact tttggcaaaa ccaaggtttc ttcaaaaagc
     5761 acccgacatg caaatttagt ttttagaatt gaaagtagct catcaaaagc agcaaatcga
     5821 ttagcaaaaa tagagttgta attggttgca tcctgcttcc cacccacatc tgccgctgct
     5881 aggacaccac ctaagtgtgg ctcatcagta ctaaccaaat tactattttc acttgttgtt
     5941 gcttcttctt tttctaaaat agtttggcta ctttttcttg ccctgcgaga tttttcaatt
     6001 cgactaggtc tactaaaact tatccatgtc ggctcagcct ctatgactcg tgtgtctgtg
     6061 tctatattgg ctgtttcctc atcgtccaac tgtagatgct catcatcaac aactgactct
     6121 gttgaaaccg ctatgtgctg cgtactacca tcacctgcct ccttttcctg aaaagaggca
     6181 tggctaatag ctgttgtgct aggaagcata gcgttgattt ctaaacctac tatttcctca
     6241 actaaataat ccttatcctc gttagaagaa cgtcctttta catgtaattt ccaaccctgc
     6301 atgggcggag ggtcaaagct aaagcaccag ctttcaacgc cgttcttaat ttctctattt
     6361 tgttgatagt gcctaaaaat actttcatac gaatccataa catcttgatc cgaaaacaac
     6421 caaaccaaaa gctgcactac ggccgactgc tctaacgccc ctttaggaaa cgatgaactg
     6481 ggtaagatcc ttatctctaa atgatctcgc tcaacctcat actgaacgtc gaactcttgc
     6541 tgtaaagcgg tactgctcaa acagcttcga cagaaatagg agttaattaa aaataatgac
     6601 cgagctagct ctaactgtgg gatgtgataa cgagtcttgc tatatggtgt ttcagcttcg
     6661 taaataaatg aactctgcgc tccatccttg ttgcgtacgg aagcaaagga cgagagatta
     6721 ggaaaatcga ttattttggc tcgttgaaga tccgatgctt caaactcaat cacacgatcc
     6781 gccttattta tttgctttgt tgtactgtta accaccttag ctcgacttaa taaaggcaaa
     6841 tgagtaaatc gagtccattt ccgttcttgc attggattaa accaaacaaa aatacgccac
     6901 tccttatgac ccgagttacg gaataaatga ccaatatgta caacctgcac attgtcatta
     6961 aatgtagcta gcctaaccac tcactcttcc ccataaacca tttccaagaa tctttgtgct
     7021 tcctcagtta tccgctcttt agataacgtt gcacttctta ataatcgcca gcgcctaagc
     7081 tcaacatctt cctgtttaag cttaataaaa gcttggctaa tccgtctaat ttgataatct
     7141 tccacactct ctgagtaacg ctttaagcaa agcgctacca aaggcagttt ctgtagattt
     7201 tttgcaagag aggttccgtt aggagtttgc tttaacagcc agctcgatgt cgctcttggg
     7261 tgatcaaggc tactatctag acgctttatg attcttaata gttgtcgtac agcaattcga
     7321 tctctttggt tccaatcaac tctaggggcg ggtgccagac gctcttgttg atgctgttga
     7381 ttccagtgaa ctagccaatc cctgtcatgt cggtaaagcc aagcgtatag caccccaccc
     7441 tctaaagact gtcttgccgc cttaattcct tggtatttat gcactagttg ctgccagtct
     7501 ttatgcttaa cagataaatc ttcagagtta ggttgcacag actggctaac aggtcttgtc
     7561 gttatagagt gctcagtaag agcacttgcc tgctgtagcg cttctataac cgttagtttt
     7621 ggcaataagg cttgccacac aatactatgc tgtaaataac taaaggcttt tctatgctta
     7681 cggaatatac ttttcagcca acacgtgtcc ttgttctctg ccaactttaa atccagtttc
     7741 tctagtgcct catcactaaa agtctgcctc actctctccg ccaccaagtc atgacgaatg
     7801 tgcttgcttt tggttagccc tagatcctgc gctaagcgct gataaaacag cgtccactgc
     7861 tcaaggcttg gggaaagctc ttgcgctcgt ggagcatcta acagaggggc tatataagca
     7921 gctagtgctg ttaattgaga tagggagtct ttggggtagt ctgaaagcag ctcagtatga
     7981 cccaaagccc aaaattgatg tcggtgatca tctacagctc tatcaaagaa gactaaagca
     8041 ccgtgttttg gacaatatgg caaagcgggc aaataccaat ctcgttgcca aaaggcttcc
     8101 ccatacctat ttagctgaag agcaacgcaa tcagggcagt atctaaagcg gttatcgctc
     8161 ttaactctag aagcagcgac tcctagcatt aaatgcaccg caccttgcgc ttggtactcc
     8221 attaaccgaa tagcttcgtc tcggcgctcc ttgcctacaa acggagcata taaagggaat
     8281 aaggtatgct cataaataag ctgctgaaca gcgtaacgtc ctgtttgatg tagatgtctt
     8341 gctatcacac ctaaatgcga gggcagacct aaggtagcga ccaccttgcg gttgccatac
     8401 acctcatcca acagctgctt aggactaaca atcccttgat aaacgcctgc ccgtgcaata
     8461 gtgctataaa tcagctcatt cgagtacgga acaggaaagt ttctcatggc taacctgctt
     8521 gtttaaataa gctcgccata tccactatta cccctttccc ttttaaccgt tcatgcatgg
     8581 ttttttcagg ttggcgttgt gaatagatat aacgtaaatc cgtatcaggc aagctatccc
     8641 agtcgctggg cttgactacc tttatagccg aaaccttttt actcttggag ggcttttcta
     8701 gttctgacac taccgtttcg ccttccatca accactgcaa aacaagaggc agtaactttt
     8761 gtcttgtcat cgttggattc tggctaaacg cttttttaat agtgggaatt aacaggcttg
     8821 aatcgtaatc ctctttcagc atcagatata aatgacgctg atcttcggta tctaactctt
     8881 gaagggcttt ttcttctggt gtttgttctt gtatcgctgc gatatctagc tgaagttgga
     8941 ttaaccgttt atcaatctcg ggaacgacta gatcagaata acgagcaatg cgttctggga
     9001 tacccgagcg taatgcctct agcatggggt gcacaggctt taactcatct tgatacactt
     9061 gccgcaataa accagcggta atacgctcat tgcctaaagc tagcgcacgg agctgagcga
     9121 gtacaaaaag ttttactaca atgtccatca ctccttggct tagctcatac cacacatcac
     9181 ggacctcatc cgataacagc gcatctttgc gttgtaaaag ctgtaattgc caaagattat
     9241 ccgtaaaagc gatccactct tgattgggct ttccacgttg cgtttgttgt ataggatccc
     9301 agaatatagc tccaaaccct gcccctctac gtgcagaccg caaatcagcc tcaaaaatct
     9361 ctcgtgcttt aggggtacca atcaacatca ctggtacgcc aataatattc accatcgtca
     9421 caaaaaagtt cagcatctct tgagatccac ccgaacgaga gcggcttaaa tgctgaattt
     9481 catcaataac caacaaccct aaagcatgtg cattggctat ttgcgacatc aaagccaaca
     9541 tggtttctat accatgacgt tttaagccat aacgacgctc atagttcgag cccaaggctc
     9601 gatccaacgc tctgaaaaaa ttcaagcaga tttcttttag cgaaccatta tgcgagcagt
     9661 ctattttcaa atacaccacc tgctctacat tgagttcacg atggtaaatc acctgaggat
     9721 acgtggctag aatacgatga agagaggtcg tcttcccact accagaacaa ccaattaaca
     9781 ataagctttg tgccgtagat cgtgcctcct caaagcgaaa tgtctccaac tctcccgttt
     9841 gaacacgctc ataaccattt tgtaaatgct tttgtaaatc tcctgtttta ggatttctgc
     9901 ctacgtagcc acctcgaatc atgaccgaaa tacgctcact tagtagcaaa tgcgtaccta
     9961 atggctgaaa atagtcatct ggaatacgac aaatggtatg agctctgata acacgggact
    10021 tttgcaagtc agaggaagta agctgtaaag aggatttcag tgatgcagca ctattcactg
    10081 actcttgtaa tggtggtaag gcctcgataa aaggattatc acgataagcc tctacccccg
    10141 tatcacgata aactgcttga atccgggtag cactcatgac tcatcctttt ctggtggatc
    10201 ctgaaataat tcaggcacgt atgtgggtag gctgtaatct tcttgatcat ccgcttccac
    10261 tgcgttgaaa ggaataactt tagcctcatc acctgaagag cttggcttca aatgctccgc
    10321 acgtttttta cgctccgagg tcacggcttc ttttttatta gttttaatct gcttaatgcg
    10381 tgttgatttg ggctcagtag tactgggcgt taacttattc gctttctgaa tggtttgctg
    10441 aataaacgcc tcaagctccc tgcgtttagt taactcatcc tgcttcgcat tggctttatt
    10501 gtgtttttct tgtgcttgta tatcccaaac ctcccaaaat gagagacctt taaactgccg
    10561 actacgttcc gtcagattac agcgccaaaa tacacggctg ccaacttgcg gaaacaaata
    10621 aatcgtatca accagcactg ggtcataagc cgcttctaaa tgttgaggtc tagctatatc
    10681 agtgctccgc tgcaaccaac cctcacgcag aatctctgac cccgagtaat acaaacccca
    10741 caaattaacg ccaaatgaag aaatagagac ctttcggcga ggcagtaacg ctactcgcaa
    10801 ctgctcttgc tccacagccc ttaaactacc tgtacgatgc tgcatacccc attgccatag
    10861 ctggacagga atagacggta aatctgtagg aaaatcagca tctcgatcgt atttatccat
    10921 caccagatgg ttatttctga ataagatcgt acgcaaaata atttgtgtga actcaaatac
    10981 cgacagagat gcatctaacc tatagtctgt ttcaccatgg cttttgatcc gactgccctc
    11041 tacaatgcca ggtgcaaagg acttaaactc ggcttgtagt gttctaaaag tgctttccac
    11101 tatgccttta gcatcgccac gtctaggtgg agcactttcc actcgcacat taaaactaga
    11161 aactaaggct tcgacctgat gactcattaa ttcgccacgg tccgctagca acacatctgg
    11221 caaacctaca cacggccagt ctgagctact aatctcaata tcatgctggg cacaaatggc
    11281 cgttttgtca gagcaagcat ttacaaaagc ctgcatcgcc accacataag acggattttc
    11341 aaagccgata taaaagcccg tgatcatccg actaaacaca tcaatcacaa tgtaaagcgt
    11401 tggtcttcct atgatttttt ggcgatcatg atgatccact aaataaatat cagcaatcgt
    11461 ggcatcaatc tcataacgac tcccagggcc taacgcctga gaagtggctg tactacttaa
    11521 gggtcgtacg tcttttttat atacccctgc tttaactcta gactttaagc gctgagcttt
    11581 agggtattct cgatcataaa aataacgaaa ctgacgtagt gttgggtaat cctcttgggg
    11641 aatgcgagga aaatactgag caaacaagtc cacaaatcgt ctataggcaa cggtggtctt
    11701 tgtacctttt tgatttaaca ggtgcttttc tatggtcaac ctaaaaaggc gttcaatctc
    11761 gggcgttacc ttggttcctt cacccttacc atattctctg gctcggccaa tctttgctgt
    11821 tcctgtcgct gaacgtcttt cccctggtgc accgctgttt ttgtagtcag gaattaatgc
    11881 attaggcgtt tgaccacgct gccagtaacg gcgtaacaac ttataaactg tagccttagt
    11941 aaccttatgt tcttggacca catgctcaac gagttcgctt ctgactttag ggtcgaaacg
    12001 atccttacta ttaataatag gaagaatttt tcgataatcc tcctcgcgct tctggaagct
    12061 gacagaatct acagacggct cttctaaatc aagatgtaca taagggtcat cagttctaac
    12121 taagcggcct tcatctaaat actgcattag caacaactca gccctagctt gtggaactcc
    12181 tttatcagcg cttatttgca tccagacaac ttggccatcc tctatagcca aaatgcgata
    12241 cggatcatta tcaaatagca caacctcatt aatttgccac atagcgcaac tcctccatat
    12301 tcactacttg gctaatacag agatctgcac acttatttgc cctgaaagac ttataaatat
    12361 tgaacttaat aaaaccattt gcggttaagg ctcgtatctc actcaatgtt ttgcctaact
    12421 ccaaatcata agcaatatca acctgcttac agacattgat aatgttttca tctccttttt
    12481 cttgcaggat atgggccaat ggggatagtt gtgctaaaag ctccgcagaa acttcttctg
    12541 ttttcactga ataaagccat tcaatatttt cttttactac gggatttatt tctttatcag
    12601 taaaaatgaa ccaaggaatt tgcttttgct gccaatagcg acgctctagt tctagttttt
    12661 ctaaggtacg ctcgtcttgt aaggctgctg caggtttgac ttgaatagca aactgctcaa
    12721 aaggaccatc tttgcagtcc actaaaaaat cagtagacat aacctgatct acaccacgaa
    12781 taacaggatg cttaatacca ctatctattg caatctgcct ggtatcacta ggtaataagg
    12841 ggaactgctc gcgtatatct agcacgctgc tctcccactc aagactgaga aaaacagcaa
    12901 gctctaagtc agatagcaaa tgatggactc gtcccgtctt atgagaataa atacggtggg
    12961 aacgacctga agaaggaact tcttgtactg ttagccatgg aatatagtct ttaccatgcc
    13021 cttggccacg cccctcttta atacgacggg caatttgcac ttcagaaaat gaagagtttg
    13081 ctttagccat aacaaaagtc cagtatgctt tttcacagca taactggact gatttcagtt
    13141 tacaactatt ctgtctagtt taagacttta ttgtcatagt ttagatctat tttgttcagt
    13201 ttaagacttt attgtccgcc cacaagccct tttttatcta tgatagtgat tactcaaccg
    13261 taaccgattt tgccaggtta cgcggctggt caacgtcggt gcctttgatc agcgcgacat
    13321 ggtaagccag cagctgcagc ggaacggtgt agaagatcgg tgcaatcacc tcttccacat
    13381 gcggcatctc gatgatgtgc atgttatcgc tacttacaaa acccgcatcc tgatcggcga
    13441 agacatacaa ttggccgcca cgcgcgcgaa cttcttcaat gttggatttc agtttttcca
    13501 gcaattcgtt gttcggtgca acgacgataa ccggcatatc ggcatcaata agcgccagcg
    13561 gaccgtgttt cagttcacca gcagcgtagg cttcagcgtg aatgtaggag atctctttca
    13621 gcttcaatgc gccttccagt gcgattgggt actgatcgcc acggcccagg aacagtgcgt
    13681 gatgtttgtc agagaaatct tctgccagag cttcaatgcg tttgtcctga gatagcatct
    13741 gctcaatacg gctcggcagc gcctgcagac catgcacaat gtcatgttca atggaggcat
    13801 ccagaccttt caggcgagac agcttcgcca ccagcatcaa cagcacagtt aactgagtgg
    13861 tgaatgcttt agtggacgcc acgccgattt ccgtacccgc gttggtcatc agcgccagat
    13921 cggattcgcg caccagagaa gaacccggaa cgttacagat tgccagcgaa ccaaggtaac
    13981 ccagctcttt cgacagacgc aggcccgcca gggtatccgc ggtttcgcca gactgtgaca
    14041 aggtgatcat caggctgtta cgacgcacgg cagatttacg atagcggaat tcagaggcga
    14101 tttcgacgtc gcacggaata cctgccagcg attcaaacca gtagcgggaa accataccgg
    14161 agttataaga agtaccacag gcgaggatct gaatatgctc aaccttcgac agcagttcgt
    14221 cggcgttcgg tcccagctcg cttaaatcaa cctgaccgtg gctgatgcgc ccggtaaggg
    14281 tgttttttat cgcgttcggc tgttcgtaga tctctttctg catgtagtga cggtaaatac
    14341 ctttatcgcc cgcgtcatat tgcagattgg attcgatatc ctgacgtttt acttccgcgc
    14401 cagttttatc gaagatgttt accgaacggc gagtgatttc cgcaatatcg ccctcttcaa
    14461 ggaagataaa gcgacgggtc accggcaaca gcgccagctg gtcagaagcg ataaagtttt
    14521 cgcccatacc caggccaatc accagcggac taccagaacg tgccgccagc agggtatccg
    14581 ggtgacggga gtccatgatc actgtaccgt acgcaccgcg cagctgcggg atagcacgca
    14641 gaacagcctc acgcagagtc ccgccttgtt tcagctccca gttcaccaga tgggcaatca
    14701 cttcggtgtc ggtttcagaa acgaaggtat agccacgcgc ttttaactct tcacgcagcg
    14761 gttcatggtt ttcgatgatg ccgttatgca ccaccacaat gtgttcagaa acatgcggat
    14821 gcgcgttagc ttctgaaggt tcaccgtggg tcgcccagcg agtgtgagca ataccggtgc
    14881 cgccatccag aggatgttct tccgctgcct gagccagcat ctggacttta ccgaggcgac
    14941 gcaggcgggt catatgacct tccgcatcaa caacggccag accggcagag tcatatccgc
    15001 ggtattccag acgacgtaaa ccttcaagaa ggatttctgc tacatcacgt tgcgcgatcg
    15061 cgccaacaat tccacacata gtttttgatt ccgatttata tcgttgtcgg tcaacctgta
    15121 tgcccgtttt ttcgggcgcc ccgagccttg tagagagtgg ggttattttt atagttactg
    15181 cttgtgggag ggagattatg ttatctcctc atcccagatg accggattac ccggccagaa
    15241 ttactttttc tttaccggac gacgccagcc ttctttctga gtctgcggca cacggctgat
    15301 agctaatgca ttttcgccga cattacgcgt cacagttgta cccgcagcaa tggtcgcgcc
    15361 tttgcctact gttaccgggg ccaccagctg agtgtcggaa ccgacaaaca catcgtcgcc
    15421 gataatggtc ttaaatttat tcgcaccatc gtagttgcag gtaatggttc ccgcgccgat
    15481 gttaacgtta tcgccaattt ccgcatcgcc caggtaagtc agatgcccga ctttcgagcc
    15541 tttacccaga cgcgcttttt tcatctcaac gaagttacca acgtgtgcac cttccagcaa
    15601 ctcagcacca ggacgcaaac gagcaaacgg gccaatagta caggccgctg ccagattcgc
    15661 gtcttccaca acggtatacg gactgatttc gcaatcatcg ccaatcacgc tgtttttaat
    15721 cacgcaaccg gtgccgattt tcacgcgatg accgagagtc acgttgccct cgatgataac
    15781 gttagtatca atttcaacat cgcgcccgtg agtaagcgta ccgcgcagat caaaacgcgc
    15841 cggatcgcgc agcataacgc ctgctaacag cagtttttca gcctgttcgg actggtaaac
    15901 gcgctccaga cgggagagtt gcaggcggtt attcacacct tctacttcgc ttaaacgttg
    15961 cggatgaacg gcgacgattt cacgcccttc ctgatacgcc agcgcaataa tgtcggtgat
    16021 gtagtattcg ccctgagcat tattgttggt cagcttcgcc agccagcgtt tcatatctgc
    16081 gccgttagcg atcagaatgc cggtgttgat ctcctgaatc tgacgctgct cgtcggtggc
    16141 atctttgtgc tcaacaatgc cggtaacttt gccattttca cgggtgatac gtccataacc
    16201 ggtcggatca tccagtttca ccgtcagcag accaatgcca ccctgcggtt tagcatcacg
    16261 caggcgctgg agtgtttcga cagagatcag cggcacgtcg ccgtagagca ttaaaatgtc
    16321 ttcatcatcg gcaaagaaag gtgcggcctg ctgcattgca tgacccgtac ccagctgctc
    16381 tgcctgaagc acccagttaa ggttgtcgtc tttcagcgcc tgtttaagca gatcgccgcc
    16441 gtgaccgtac accaagtgaa cgtgcgctgc gcctaattca ttcgcagcat caatgacatg
    16501 ctgaaccatc gctttcccgg caagggtatg cagcactttc ggaagatcgg aatacatgcg
    16561 cgtgcctttg cctgcggcaa ggatcactac gctcatagca ttattcaaca tacgcgtcct
    16621 gactgtaatt tgagaacgaa tttaaaccgc ttcaccttga aaaaactaca tttttttcat
    16681 cgtgaaatgg acagaggata aattgttcaa taacggatta tccccgctga cgaaagtgcc
    16741 attttcgacc atcgtcacgc cttttgtctg cagaaaatta aggcagtacc ttcatttaag
    16801 cgtcaggtgg atgtttttgc tcttatttcg atcaatgaat aaacgggaaa acaggggtgt
    16861 tttatatcag caggatctat gtgaacgctc ttcaggacgg atcacgcgta aaaaaaagcc
    16921 agcctgtttc cagactggct tttgtgcttt tcaagccggt gttacatcgc ttttttggtc
    16981 aactcgataa cgcgcagctg cgcgatcgct ttggccagtt ccgcagacgc ctgagcgtaa
    17041 tctacgtcgc cgtgagagct gctaatgtgc tcttcagcct tacgtttcgc ttccatggct
    17101 cgcgcttcgt cgagatcctg gccgcgaatt gcggtgtcgg ccagaacggt cacgttgcca
    17161 ggctgcactt caagaatgcc gccagacaga tagataaact cttcgtgacc gtgctgtttc
    17221 acgatgcgaa tcataccagg cttaatggcg gtgagcagcg gtgcgtggcc agggtagatc
    17281 cccagttcac cttcgctacc cgttacctgg attttctcga ccagaccaga gaacatttgt
    17341 tgctctgcgc tgacgacgtc caggtggtaa gtcattgcca tatcaccctc cgattaaggc
    17401 gttaaagttt tttggctttt tccacagctt cttcgatgga accgaccatg tagaacgcct
    17461 gctccggcag gtgatcgtat tcgccttcca tgatgccttt aaagccacgg atggtgtctt
    17521 tcagggagac gtatttaccc ggagaaccgg tgaatacttc tgccacgaag aacggctggg
    17581 acaggaagcg ctggatctta cgagcacgcg ctaccaccag tttgtcttct tcagacagtt
    17641 catccatacc caagatggcg atgatgtctt tcagttcctg ataacgttgc aggatggact
    17701 gaacgccacg cgcggtgtcg tagtgttcct gaccaaccac cagcgggtcc agctgacggc
    17761 tggtggagtc cagcgggtca acggccgggt agatacccag agacgcgatc tgacggctca
    17821 gtaccacggt tgcgtcaagg tgcgcaaagg tggttgccgg agacgggtca gtcaagtcat
    17881 ccgcaggtac gtataccgcc tgtacggagg tgatagaacc agttttggtg gaggtgatac
    17941 gttcctgcag aacgcccatc tcttccgcca gggtcggctg ataacctacc gctgaaggca
    18001 tacggcccag cagtgcggat acttccgtac cggccagggt gtaacgatag atgttgtcaa
    18061 cgaacagcag aacgtcacga ccttcgtcac ggaatttctc agccatggtc agaccggtca
    18121 gagcaacgcg cagacggttt cccggcggct cgttcatctg gccatacacc agggatactt
    18181 tgtcgataac gttggagtcg gtcatttcgt ggtagaagtc gttaccctca cgagtacgtt
    18241 cacctacgcc cgcaaacaca gagtaaccgg agtgctcgat cgcgatgtta cgaatgagct
    18301 ccatcatgtt tacggtttta cctacacccg caccaccgaa cagaccaact ttaccgccct
    18361 tagcgaacgg acacatcagg tcgataactt tgataccggt ttccagcagt tcctgagagt
    18421 ttgacagctc ttcgtaggaa ggtgctgcgc ggtgaatcgc ccaacgctct tcttcaccga
    18481 tctcgccttt catgtcgacc ggttcaccca gtacgttcat gatacggccc agagtcgctt
    18541 tacctaccgg gacttcgatc gggtgttcga ggtcttttac atccagaccg cgacgcagac
    18601 cgtcggagga acccattgcg atggtacgca cgataccgcc gccgagctgc tgctgaactt
    18661 ccagcaccag acgctcatta ccattttgca cctcaagagc atcgtacacg cgcggtacgg
    18721 catcctgagg gaattcgacg tcaactacgg cgccgattac ctggacaatc tttccagtag
    18781 ccatcttaaa tcctctacga aataacctgt ttaaaccgcg gcggcccccg agacgatctc
    18841 ggtgagttcc tgagtaatgc tggcctgacg agctttgttg tataccaact gcagctcttt
    18901 aatcaggctg ccgccattgt cggtcgcggc tttcatcgcc accatacggg cggcctgctc
    18961 gctggccagg ttttcaacca cgccctgata aacctgagat tcgacataac gacgcagcag
    19021 ggtatccagc aacgccttcg gatcgggttc gtacaggtaa tcccaggatt tatgtttcag
    19081 atcatcatca tctgatgccg gtaacggcag cagctggctg atggtcggaa cctgagacat
    19141 ggtgttaata aatttgttgc tgacaatgta aagcttgtcc agacggcctt cgtcgtaagc
    19201 ctgcaacatc acttttaccg gaccgatcag ttcggacagg gaagggttat cccccatgcc
    19261 ggtgacctgg gcaacaacat tgccgcccac ggagttgaag aacgacacgc ctttcgagcc
    19321 gatcattgcg aggtcgcatt gaacgctttt gtcggtccag gtcttcattt ccgccagtag
    19381 ttttttgaac aggttaatgt tcaaaccacc gcacaaacca cggtcggtcg acaccaccag
    19441 gtagcccacg cgtttaacgt cgcggtcttc caggtaaggg tgcttatatt ccagattacc
    19501 gtgtgcaagg tgaccaatca ctttgcgcat ggtttctgca taaggacggc tggccgccat
    19561 gcgatcctgc gatttacgca ttttggaagc ggcgaccatc tccatcgctt tagtgatctt
    19621 ttgcgtgttc tggacgcttg cgatcttact acgtatctct tttgcgccgg ccatgagctt
    19681 ctcctcaatg ccttgcggcc tgccctaagg caagccgcca gacgttacca ggattgggtt
    19741 gctttgaagg aatcgaggat gcctttcagc ttgccttcga tttcgtcgtt gtagccaccg
    19801 gtctggttga tctcttgcat caacggagcg tgatcacggt cgacgtaagc cagcagagcg
    19861 gcttcgaagc tgccaatttt cgacagttca acatccgcca ggtaaccacg ttctgctgcg
    19921 aacagaacca gagactgctg cgcaacggac atcggcgcat actgtttctg tttcagcagt
    19981 tcggtcactt tctgaccgtg gtcaagctgc ttacgtgttg catcgtcaag gtcggatgca
    20041 aactgagaga acgctgccag ttcacgatac tgtgccagag cggtacggat accaccggac
    20101 agttttttca tgatcttggt ctgtgctgca ccaccaacac gggatacgga aatacccggg
    20161 ttaaccgcag gacgaatacc ggcgttgaac aggttggttt ccaggaagat ctgaccatcg
    20221 gtaatggaga ttacgttggt cggaacgaac gcagaaacgt cacccgcctg agtttcgata
    20281 atcggcagtg cggtcagaga accggttttc cctttcactt cacctttggt gaaggcttca
    20341 acgtattcgg cgttaacacg tgcagcacgc tccagcagac gagagtggag gtagaaaacg
    20401 tcgcccggga atgcttcacg tcctggcgga cgacggagca gcagggagat ctgacggtaa
    20461 gcaacagcct gtttagacag gtcatcgtaa atgatcagcg catcttcacc gcggtcacgg
    20521 aagtattcgc ccattgcgca accggcatac ggtgccaggt attgcagtgc agcggattca
    20581 gacgcggttg ctaccacaac gatggtgtta gccagtgcgc cgtgctcttc cagtttacgt
    20641 accacgttag aaatggtgga cgctttctgg ccgatagcga catagataca tttgataccg
    20701 gaatcgcgct ggttgatgat ggcatcgata gccagtgcgg ttttacctgt ctgacggtca
    20761 ccgatgatca attcacgctg accacgaccg attgggatca tggagtcaac ggctttataa
    20821 ccggtctgta ccggctgatc tacggactga cgttcgataa cgcccggagc gattgcttct
    20881 acagcagaga agccgtcgtg atccagcgga cctttaccgt cgattggtgc acccagagtg
    20941 ttaaccacac ggcccagcag gccacggcca accggaactt ccaggatacg gccagtacac
    21001 ttaactttca tgccttcggc aaggtcagcg tacggaccca taacaaccgc acctacagag
    21061 tcgcgctcaa ggttcagtgc gatagcgtaa cggttacccg gcagggagat catttcaccc
    21121 tgcatacaat cggccaggcc gtgaatgcgg ataacaccgt cacttacaga aacaatagta
    21181 ccttcgttgt gagcttcact cacaacattg aactgagcaa tgcgctgctt gatcagttcg
    21241 ctgatttcgg tggaattcag ttgcatgctc cagtcccctt aagactgcaa gacgtctgca
    21301 aggcgctcaa gacgaccgcg tacgctgcca tcaatgacca tatcacccgc tcggatgata
    21361 acgcctgcca ttacagactt atcgattttg caattcagct taactttgcg tgacagacgt
    21421 ttttccatcg cagcagaaat tttcgcgagc tgttgttcac tcagtgcggc agcggaaatg
    21481 acgtctacct cagcggtagc ctcactcacg gcacgaaggt gaataaactg ctccagaaca
    21541 tccgggagcg cgttaagacg accattttca gccattaccc gaatcaggtt ctgaccgttt
    21601 tcgtccagtt gctcaccaca aactgcgata aacgactcgg cgagcgtttc tggcgcaagc
    21661 gcgccagaga gaagctctgc catttgttcg tttttggtta cctcggcggc aaacgccagc
    21721 atgtcctgcc agcgttctac actttggtgt tcgacggcaa agtcaaaagc tgctttggcg
    21781 taggggcgag ctaccgtaat aaattcagac atcagcccct ccctccttac agttcagcga
    21841 caagtttatc cacgatgtcg ctgttagcag cttcatccac ggaacgttcg atgatcttct
    21901 cggcgccagc aacagccagg atagcaactt gcttacgcag ctcttcacgg gcacgtttac
    21961 gctcggcttc aatttctgcc tgcgcctggg ccacgatttt agtacgttcc tgttctgcct
    22021 cagctttcgc ttcgtccaga atctgcgagc ggcgtttgtt cgcctgctcg atgattacct
    22081 gggcttccgc tttcgctttt ttcagctggt cggtcgcgct ggcctttgca aggtcaaggt
    22141 ccttatgtgc tcgttctgcg gaagcaaggc cgtcagcaat ttctttttga cgtttttcga
    22201 tggctgccat taatggcggc catacgtact tcatgcagaa cagaacgaac aggacaaacg
    22261 cgatggcctg gccgaggatt gttgcgttaa gattcacagc acaatgcctc tatttagtta
    22321 acgttctgat attgctcttt aaataaaagc aacgcttact acgcgacagc gaacatcacg
    22381 tacagaccca gacctacagc gatcatcggg atagcatcca ccagacccat aacgataaag
    22441 aactgagtac gcagcagagg aatcagatca ggttgacgcg ctgcgccttc caggaattta
    22501 cccccgagga tgccgatacc gatcgcagca ccgattgccg ccagacccat catcacagcg
    22561 gcagccatgt acagcagatc catattcagg ttttccatga cagtctccag tttgtttcag
    22621 ttaaaacgta gtagtgttgg taaattaatg ttcttcagac gccatcgaca gatagacgat
    22681 cgtcagaacc atgaagatga aggcttgcag cgtaatgatc aggatgtgga aaatggccca
    22741 cggcacattc aggatccact gtgaccacca cggcaacaga ccagcaatca gaatgaaaat
    22801 cagctcaccg gcatacatgt taccgaacag tcgcaaaccg agtgaaactg gtttggacag
    22861 caggcttacc ccttcaagga ttaagttgac aggaatgaac gcccagtgat tgaacggctg
    22921 cagcgtcaac tctttcgtga agccgccgat gcctttcatt ttgatgctgt agaacagaat
    22981 caggataaat acgcccagtg ccatagacag cgttacgttc acgtccgcag acggaaccac
    23041 acgcagtgca ggcagaccca gtacatgttc agcaatgtac ggcagcaggt cgataggcag
    23101 taaatccatc aggttcatca ggaataccca gacgaagatc gtcagggcca gcggagcaat
    23161 cagcttgctt ttgccatggt acatgtcttt cacgctacca ttaacaaagc cgatcaccag
    23221 ctcaatcgcg gtctgaaact tacctggcac accgctggtc gcctttttgg ctacgctacg
    23281 gaataaaacc aggaacaaca gacccagcac caccgagaag aacatggagt caatattgat
    23341 tgtccagaag gtggctgggg ggttttgtgg atccaccagc gagaatgtac gcaggtccag
    23401 ctgaaggtta ttcaggtggt gtcctatgta atcctgcggc gtcatatttt ctgaagccat
    23461 gatgcctttt accctttgtt gttaattaca gccggtgcca gtatctgaac caccagcacc
    23521 aaaacccacg taacgatcag cggcaagaat accgccttta aaaccgccaa cgccaccacc
    23581 agtaacacca acatcgccag aactttgaaa gcttcgccaa atgcgaatgt ccaggccacc
    23641 cggcctttcg ctggtgtata cgcctggtga cgccaggcaa atatcataaa caaaacgtta
    23701 ggcagaaaga ctgccaggcc cccgcttatt gcagagacgc cccagaaggg gtctttgagg
    23761 ctgaacagca atccacttgc tatcaccacc agtaactgaa cgagcagaag cttccgagca
    23821 acgtttcgac tcacgagcga cacagacatc acgtttttca ctcctgctcc cttcgaggta
    23881 tgccgcgtgt cgtataaaac tttctttaag gcttagagtc aagcatcaaa aagcggtcaa
    23941 attatacggt gcgcccccgt gatttcaaac aataagtagc caaaaggtga ataaatgttt
    24001 aaatattttt ccagtgcata caattgcgac ttttccgcta accctgttcg atcatgaaaa
    24061 actgtaaata acgcgtaaac actagtgata aagcgtgctt cagatcacat attgcgcatg
    24121 ttcgcgcaca gtatatttat ttacttggca aatg
//
LOCUS       DAZPAC010000049        23771 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZPAC010000049 DAZPAC010000000
VERSION     DAZPAC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23771
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.049"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(506..1303)
                     /locus_tag="ACHUPM_001609"
     CDS             complement(506..1303)
                     /locus_tag="ACHUPM_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0869422.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1313..1864)
                     /gene="ybcL"
                     /locus_tag="ACHUPM_001610"
     CDS             complement(1313..1864)
                     /gene="ybcL"
                     /locus_tag="ACHUPM_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR0869423.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(2033..2365)
                     /gene="emrE"
                     /locus_tag="ACHUPM_001611"
     CDS             complement(2033..2365)
                     /gene="emrE"
                     /locus_tag="ACHUPM_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR0869424.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            2546..2674
                     /locus_tag="ACHUPM_001612"
                     /pseudo
     CDS             2546..2674
                     /locus_tag="ACHUPM_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2699..3013)
                     /gene="fliE"
                     /locus_tag="ACHUPM_001613"
     CDS             complement(2699..3013)
                     /gene="fliE"
                     /locus_tag="ACHUPM_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR0869425.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3229..4887
                     /gene="fliF"
                     /locus_tag="ACHUPM_001614"
     CDS             3229..4887
                     /gene="fliF"
                     /locus_tag="ACHUPM_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFR0869426.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4880..5875
                     /gene="fliG"
                     /locus_tag="ACHUPM_001615"
     CDS             4880..5875
                     /gene="fliG"
                     /locus_tag="ACHUPM_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR0869427.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5868..6554
                     /gene="fliH"
                     /locus_tag="ACHUPM_001616"
     CDS             5868..6554
                     /gene="fliH"
                     /locus_tag="ACHUPM_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR0869428.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6554..7927
                     /gene="fliI"
                     /locus_tag="ACHUPM_001617"
     CDS             6554..7927
                     /gene="fliI"
                     /locus_tag="ACHUPM_001617"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR0869429.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7946..8389
                     /gene="fliJ"
                     /locus_tag="ACHUPM_001618"
     CDS             7946..8389
                     /gene="fliJ"
                     /locus_tag="ACHUPM_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFR0869430.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8386..9513
                     /locus_tag="ACHUPM_001619"
     CDS             8386..9513
                     /locus_tag="ACHUPM_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR0869431.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            9618..10082
                     /gene="fliL"
                     /locus_tag="ACHUPM_001620"
     CDS             9618..10082
                     /gene="fliL"
                     /locus_tag="ACHUPM_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR0869432.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            10087..11091
                     /gene="fliM"
                     /locus_tag="ACHUPM_001621"
     CDS             10087..11091
                     /gene="fliM"
                     /locus_tag="ACHUPM_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR0869433.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            11088..11501
                     /gene="fliN"
                     /locus_tag="ACHUPM_001622"
     CDS             11088..11501
                     /gene="fliN"
                     /locus_tag="ACHUPM_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR0869434.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11504..11869
                     /gene="fliO"
                     /locus_tag="ACHUPM_001623"
     CDS             11504..11869
                     /gene="fliO"
                     /locus_tag="ACHUPM_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR0869435.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            11869..12606
                     /gene="fliP"
                     /locus_tag="ACHUPM_001624"
     CDS             11869..12606
                     /gene="fliP"
                     /locus_tag="ACHUPM_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR0869436.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12616..12885
                     /gene="fliQ"
                     /locus_tag="ACHUPM_001625"
     CDS             12616..12885
                     /gene="fliQ"
                     /locus_tag="ACHUPM_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR0869437.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12894..13679
                     /gene="fliR"
                     /locus_tag="ACHUPM_001626"
     CDS             12894..13679
                     /gene="fliR"
                     /locus_tag="ACHUPM_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR0869438.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            13964..14587
                     /gene="rcsA"
                     /locus_tag="ACHUPM_001627"
     CDS             13964..14587
                     /gene="rcsA"
                     /locus_tag="ACHUPM_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR0869439.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14631..14819)
                     /gene="dsrB"
                     /locus_tag="ACHUPM_001628"
     CDS             complement(14631..14819)
                     /gene="dsrB"
                     /locus_tag="ACHUPM_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR0869440.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14982..15209
                     /gene="yodD"
                     /locus_tag="ACHUPM_001629"
     CDS             14982..15209
                     /gene="yodD"
                     /locus_tag="ACHUPM_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR0869441.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15507..16322
                     /locus_tag="ACHUPM_001630"
     CDS             15507..16322
                     /locus_tag="ACHUPM_001630"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFR0869442.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(16319..18013)
                     /gene="dgcQ"
                     /locus_tag="ACHUPM_001631"
     CDS             complement(16319..18013)
                     /gene="dgcQ"
                     /locus_tag="ACHUPM_001631"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFR0869443.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(18184..18366)
                     /locus_tag="ACHUPM_001632"
     CDS             complement(18184..18366)
                     /locus_tag="ACHUPM_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR0869444.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(18445..19362)
                     /locus_tag="ACHUPM_001633"
     CDS             complement(18445..19362)
                     /locus_tag="ACHUPM_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR0869445.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            19535..20456
                     /gene="yedA"
                     /locus_tag="ACHUPM_001634"
                     /pseudo
     CDS             19535..20456
                     /gene="yedA"
                     /locus_tag="ACHUPM_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            complement(20445..20915)
                     /gene="vsr"
                     /locus_tag="ACHUPM_001635"
     CDS             complement(20445..20915)
                     /gene="vsr"
                     /locus_tag="ACHUPM_001635"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR0869446.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20896..22314)
                     /gene="dcm"
                     /locus_tag="ACHUPM_001636"
     CDS             complement(20896..22314)
                     /gene="dcm"
                     /locus_tag="ACHUPM_001636"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR0869447.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(22381..23037)
                     /locus_tag="ACHUPM_001637"
     CDS             complement(22381..23037)
                     /locus_tag="ACHUPM_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFR0869448.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            complement(23077..23358)
                     /gene="drpB"
                     /locus_tag="ACHUPM_001638"
     CDS             complement(23077..23358)
                     /gene="drpB"
                     /locus_tag="ACHUPM_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR0869449.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5913 a         6159 c         5906 g         5793 t
ORIGIN      
        1 atctacttca caatttaaat gagaaactaa aacttacatc ttgaaataat ctcattgatt
       61 ggatgaatat ttgtagtgca gtgacatcat ttttaaataa tagttcaaat aaaagggctc
      121 acgatgaaaa aattaacagt ggcaatttct gctgtagctg catcttaacg tataaactag
      181 atttaataat aacgaactag atttaataac aacgatatcc gtacctatcc ggatatctaa
      241 tgtcataagc atccctttaa ttatcccctc aacctcttgt aactttccca ctcttgaact
      301 ccacatagtc caaacgccgg atgcaaaaaa atcacacgtg catttctgaa attgtgatgg
      361 aggaagatag tatcaattat atgtgcttat aaacgtcccc gattagcgca gaagtcaccg
      421 gattgttcag gctccaatag gttgagtatg ccgtatttat tttccaaaat aaaaatcgat
      481 gctgttgctc cttcataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg
      541 ttatcttaaa acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt
      601 ccctggcaat ctcgctgatg ggttttactg tcgtttgtat agcagacaac gcattattta
      661 acctcacgtc gtccaatata ctttgaaaac ttactccctc gcttgccaga cggcgatgca
      721 atgtggaaac agaaatgtag agatatctcg ctaccttgtt tgctgtccat tttgttccag
      781 gttcggatag cattaggtta taacaacgac ttattaatga ttgtttacta tctgataaaa
      841 gtaaacaatt aacatgatgt gctcctaatg aaagtagaac gcccattgct aagtgttcct
      901 gaatttgcgt tgagaagcct cgggaaatag atgtttttag ttgctcccaa caatatatta
      961 actcggaatt ctgaggtaag cagaaacctg tcgtgttacg aatttgatcg gttatcgcat
     1021 aaagattttg gaacttttca attagatcaa tgggataata aagcatttct gcaagataaa
     1081 gacctgcttc tgggtaattc gcaatataaa attcatatcc acagggaaat aatattatct
     1141 gattattatc aacagttaga ctatgcgtct cccaattgat aacttttttt ccctgacgga
     1201 tacgacatga agctggcata agaggcttaa ccctgtgaat ctcatgatgt ttatgcatcc
     1261 gtatttcttc aatctttagg ttagtcctat cttttgccag catactctta tcctacttta
     1321 cctcataaac gggtattatc tcagcggttg caattttatt ggcattaagc atataaccaa
     1381 ctaacgctcc gctggaatta gaatccacag gaatcttatc agtttttaga gcccatattt
     1441 taaactggta atgatggggt ttatctcctt tgggaggaca tgctccacca aatcccgcat
     1501 agccaaaatc atttcggcct tggacagcac cagtcggaag ttttgtacca tcacgtcttc
     1561 ctgcatcagc gggcaaatat gttaccgttg ctggaatatt agcaacagtc cagtgccacc
     1621 atccgctgcc tgtaggcgca tctggatcat acacagttac ggcaaagctt ttggtgcctt
     1681 ctggagcacc agaccaggtt aatgagggcg atgtattacc cccctcacac ccaactccag
     1741 aaaatacatg agacgttgta agttgctctc ctgtttttat ttcattacta gtgacctgaa
     1801 atgctgcggc ctgcgcagaa aatgttatga atgccaatac acttgaaacg ataagttttt
     1861 tcataaaaac ctctttgttg tgacctatcg ttattttatt tgatgtgcat tgatctcatt
     1921 atgcaaaaag gcgcaatgat cgcgcaaatg caatcataat tataaagttg ccccgaacat
     1981 tagccataat atgcacaaat cagattcctg ttatctcaag gatgattatt ttttaatgcg
     2041 gtgcgcttcg tgacaacaag ttaatgacta acacaccggc acaaatcaac atcatgccta
     2101 tgatggctgg caggtccagt cgttggccaa aaaatcccca ggaaagcaaa ctaatcagaa
     2161 cgataccgac tcctgaccag atagcataag caatccctgt aggtatataa gccagtgtct
     2221 gagctaataa ccaaaatgat gcacaatagc aaataattgt gccaacagag ggccataacc
     2281 gtgtaaaccc ctctgaaaac ttcattaagg ttgtaccaat gacctctgca agtattgcgc
     2341 caccaagata aatataaggg ttcatagcat attctttcct gttcatactg gagagaattg
     2401 tactacagtt tgaacacaac tcaccgtttt catcctggtg tatgtgtatc cagggggctc
     2461 tggcgtggca agagataaag gcaggaacaa taatgatcag aaggaacccc aggctaacaa
     2521 aagagttcac tcttcctatg agcaaagcga agacaaaaca tgttatcgcg tttacggatc
     2581 gtggatggca gaaaactttc aggatcaggt atccatttta aaccagaaat tgagtgagtt
     2641 tgccccatcc atgccccacg cggtgagatc ggatgtaatt aacaacaggt tacaaaacct
     2701 acacctgcat ggacatcact tcctgataag ccgccaccag cttattacgc acctgaatcc
     2761 ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt aacgccacgc
     2821 cgggttcacc gagagtgaat ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta
     2881 tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgt ggcagtgatt
     2941 cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct
     3001 gtatcgctga cattttcatc tcccggataa tttctggtag caaagcttac cagtaagtca
     3061 ataagacaaa ggcgctaaat agcaacaaaa aaaacgggtt tattggcgga tagaaaaaaa
     3121 cgaaagcaca aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt
     3181 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
     3241 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
     3301 ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
     3361 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
     3421 agccaactga cgcaaatgaa tattccttac tgcttcagcg aagccagcgg cgctattgaa
     3481 gttccggcag ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa
     3541 ggcggcgcgg tcggtttcga actgcttgat caggaaaagt ttggtatcag ccagttcagc
     3601 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
     3661 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
     3721 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
     3781 gaagggcaaa ttagcgccat tgtgcacctg gtttccagcg ccgttgctgg tctgccgccg
     3841 ggaaacgtca cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg
     3901 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg
     3961 cgtattgaag caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg
     4021 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
     4081 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
     4141 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
     4201 atcagcacgc ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
     4261 agcgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
     4321 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
     4381 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
     4441 gaagatctga cccgcgaagc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc
     4501 gttaactcgc cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag
     4561 caagcgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg
     4621 tggctgctgt ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa
     4681 gctgtacagc aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg
     4741 agcaaagacg aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg
     4801 agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc
     4861 cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct
     4921 gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt
     4981 gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccta
     5041 tgtgctggcg gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa
     5101 cgattatctg cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct
     5161 ggaagatatt ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga
     5221 gccgcagagt gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct
     5281 ggtgcatctg aagcgcgcgc aggccgccga tattctggcg ttgttcgatg aacgtctgcg
     5341 ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga
     5401 actgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg
     5461 cggcgtgaga acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt
     5521 tattaccgcc gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt
     5581 cctgttcgaa aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt
     5641 ggattccgaa tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt
     5701 cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg
     5761 tccggtgcgt ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg
     5821 ccttgccgaa actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc
     5881 tgccgtggaa aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca
     5941 tggtcgagcc ggaagaaacc atcattgaag aggccgaacc tagccttgag cagcaactgg
     6001 cgcaactgca aatgcaggcc catgagcaag gttatcaggc ggggattgcc gaaggtcgcc
     6061 agcaaggtca tgagcagggc tatcaggaag gactggccca ggggctggag caaggtctgg
     6121 cagaggcgaa gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat
     6181 ttcaaactac ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc
     6241 tggaggcggc acgtcaggtg atcggtcaga cgccaacagt ggataactcg gcactgatca
     6301 aacagatcca acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg
     6361 tgcacccgga tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta agtttgcatg
     6421 gctggcgctt gcggggcgat cccacactcc atcctggcgg ctgtaaagtc tccgccgatg
     6481 aaggcgatct cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac
     6541 caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga
     6601 agccaaaatg gcgcagttgc ctgcggtacg tcgctatggg cgattaaccc gcgctaccgg
     6661 gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgaacg
     6721 ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg
     6781 gctgttttta atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gggtttatgc
     6841 caaaaacatt tcggctgaag gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt
     6901 attaggtcgc gttctggacg gcagcggtaa gccgctcgat ggcctgccct cccccgatac
     6961 gacggaaacc ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga
     7021 acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgtgggca
     7081 gcgtatgggg ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc
     7141 acgttacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt
     7201 aaaagatttt attgagaata tcctcggtgc tgaagggcgt gcacgctccg tggtgattgc
     7261 cgctccggcg gatgtctctc cgcttctgcg aatgcaaggt gccgcctatg ccacgcgcat
     7321 tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg
     7381 ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccaccaaagg
     7441 ttatccaccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat
     7501 tagcggcggc ggctcgatta ccgcgtttta taccgtgctt actgaaggcg atgaccagca
     7561 ggaccccatt gcagactccg cacgggcgat cctcgacggt cacattgtgc tgtctcgccg
     7621 actggcggaa gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat
     7681 gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc
     7741 gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc
     7801 gatgctcgat aaagccatcg ccctgtggcc gcagctggag ggctatttac aacaaggcat
     7861 ttttgaacgt gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt
     7921 gtcataaccc aggagataac ggtagatggc agaacatggt gcgctggcga ccctgaaaga
     7981 tctggcagaa aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg
     8041 tcagcaggcg gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa
     8101 cctcaacagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt
     8161 tatccagacg ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca
     8221 gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca
     8281 gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa
     8341 aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg
     8401 cccttgatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct
     8461 gcacaagatt ttctcgcgtt gttgagcgaa gcattagcaa gcgagacaac taccgacaaa
     8521 gcggcccccc aggtgctggt ggcaacagat aagcccacga caaaaggcga gctgctgatc
     8581 agcgatattg tttccgacgc gcaacaagcg gatttactga tccctgtgga tgaaacactg
     8641 cctgtcatca acgtcgaaca atccacatcg acaccgttaa ccaccgctca tacgatgacg
     8701 ttggctgcgg tggctgacaa aaatacgaca aaagacgaaa aagcggatga tctgaatgaa
     8761 gacctcaccg caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc
     8821 aaagtgactg atgcgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa
     8881 ctgacttctg cgcaactcac aacagcacag cctgatgatg cccccggcac gccagctcag
     8941 ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca
     9001 ccggtgaccg ctgccgccag cccgctaatc actccacacc agacacagcc actgctcacc
     9061 gtcgccgcgc ctgtcttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc
     9121 cagcatattt cgctgttcac ccgccaggga caacaaagtg cagagttgcg tctgcacccg
     9181 caggatttag gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag
     9241 atggtttcgc cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt
     9301 acgcagctgg ccgagagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt
     9361 agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa
     9421 cctctggcgg gggaagaaga cgatacgctt ccggttcccg tctctttaca agggcgcgta
     9481 acaggcaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt
     9541 cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca
     9601 caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt
     9661 ggctcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact
     9721 ggcattcgca tcaggttgcc gctgaagaca aagcgcagca tcgcgtcgtg ccctcaccgg
     9781 tcttctatgc gctggatacc tttacggtca atttgggcga tgcggatcgc gtgctttata
     9841 tcggtataac cctgcgactc aaagatgaag ctacccgctc gcggctgagt gagtatttgc
     9901 cggaagtccg tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga
     9961 cagaagaagg caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg
    10021 ttgccgggca accgaaacag gatgtcaccg acgtgatgta taccgctttt attctgcgat
    10081 aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac
    10141 agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac
    10201 gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat
    10261 gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat
    10321 ataaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg
    10381 ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt
    10441 tcaccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcaatgg acgcttcccg
    10501 accaaagtgg aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta
    10561 aagctggcgc ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag
    10621 tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt
    10681 gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt
    10741 ctgccattca gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac
    10801 tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag
    10861 ctggagctgg tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg
    10921 aaacccggcg acgtcctgcc gatagaaaaa cccgatggca tcatcgccca tgttgacggc
    10981 gtcccggtgc tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa
    11041 catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga
    11101 ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg
    11161 aacaaaaatc aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg
    11221 gtgatgtcag cggaacgttg caggatatcg acctgataat ggatattccg gtcaagctga
    11281 ccgtcgagct gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt
    11341 ccgtcgtggc gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt
    11401 taatctccca gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata
    11461 tcattactcc gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata accacgctac
    11521 tgtgcaatct tccacgccgg tttctgctgc gccactgctg caggtcagcg gcgcactcat
    11581 cgccattatt gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgcccc
    11641 gaaacgcact ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga
    11701 acgggttgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca
    11761 aatcaatctg ctgcataaac ttcccccttc agcaccaaca gaagagatac cgcagaccga
    11821 ttttcagtcg gtcatgaaaa atttgcttaa gcgtagtggg agatcctgat gcgtcgtttg
    11881 ttgtctgtcg cacctgtcct tctctggctg gttacgcccc tcgccttcgc gcaactgccg
    11941 ggtatcacca gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg
    12001 ctggtgttca tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc
    12061 acccgcatca tcattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca
    12121 cctaaccagg tattgctggg gctggcacta tttttgacct tttttattat gtcatcggtg
    12181 atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag
    12241 gaggcgctgg aaaaaggggc gcagccgcta cgtgagttta tgctgcgtca gacccgtgag
    12301 gcagatttag ggttgtttgc cagactagcg aataccggcc cgttgcaggg acctgaagcc
    12361 gtgccgatgc gcattttgct cccggcctac gtgaccagcg aattgaaaac cgcatttcag
    12421 ataggcttca cgattttcat tccttttttg attatcgacc tggtgattgc cagcgtgttg
    12481 atggcattgg ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg
    12541 ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac
    12601 agctagagag gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa
    12661 gtcgcgctgg cactggctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc
    12721 agtattttgc aggccgccac gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc
    12781 atcgccgtat ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat
    12841 tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtaggccgt acaatgatgc
    12901 aggtgacaag cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg
    12961 tgctggcgct catttctacc gcaccaattc tgagcgaacg cagcgtgccg aaacgggtaa
    13021 aactggggct ggcaatgatg atcacgttcg tcattgcccc atcattacct gccaacgatg
    13081 ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg
    13141 cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg
    13201 gtctgcaaat ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc
    13261 ccgttttagc gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc
    13321 atttatggtt gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac
    13381 cgttgaacag caatgcgttt ctggcactca ccaaagcggg gagtttgatt ttccttaacg
    13441 ggctgatgct ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt
    13501 tactgaatcg tatggccccg caattatcca tttttgttat tggatttcca ttaactctga
    13561 ctgtcggtat ctctttaatg gcggcattaa tgccgttaat tgcacctttt tgcgaacatt
    13621 tattcagtga gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat
    13681 tccgtaacgt ttatcatgtt atcctaagga ttatccgaaa aataatacct acgaacatct
    13741 tccaggatac tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta
    13801 ctttaagaca attccaggca aattatacaa cactttacgg gatagtaagt ccgcctgaaa
    13861 aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg tttagtcatg atgaaatatt
    13921 caggtaaggg gaattatcgt tacgcattga gtgagggtat gccatgtcaa cgattattat
    13981 ggatttatgt agttacaccc gactaggttt aaccgggtat ctgttgagta gaggggttaa
    14041 aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt gccatagctt gtgattcaca
    14101 gcgcccttca gtggtgttta ttaatgagga ctgtttcatc catgatgctt ctaacagtca
    14161 gcacatcaag cacatcatta atcaacatcc caatacgtta tttatcgttt ttatggcaat
    14221 tgccaatgtt cattttgatg aatatctatt ggtcagaaaa aaattattga taagttctaa
    14281 atcgattaaa ccggaatctc tcgacgatat ccttggcgat attctgaaaa aagagacaac
    14341 gataacctct tttttaaata tgccgacgtt atcattgagc cgaaccgaat cgagtatgtt
    14401 gcgaatgtgg atggcaggtc agggaaccat tcaaatctct gaccaaatga atatcaaagc
    14461 caagaccgtt tcatcgcata aaggtaatat taaacgtaag atcaaaacac ataataaaca
    14521 ggttatctac catgtcgtcc gactgacgga taatgtgact aatggtattt ttgtcaacat
    14581 gcgctaacac attctgactg gtggtttccc accagtcagg ctgaataaga ttactctgct
    14641 ttctccacaa agataccgtc ctgatgccct gcttcattaa agaaccagat gccgagcggg
    14701 tagtcttcca gcgaaaccag gtacattgtg ccttcactaa actcctcaac tgccagtacc
    14761 acgccaggac gacgcggacc gccatccgtt ttgactgtta cccgatcatt caccttcatc
    14821 gttttcctcc tgtggctttg tgccagtgta gaacaatttc gtttttctgg cagctccagg
    14881 cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag agatgccagg cggacttaac
    14941 gactggcggc aacaacagag taacggttgc gaggaaagat gatgaaaacc gcaaaagagt
    15001 acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga tgccctgctg gcggcgatca
    15061 atgaaattag cgaaagcgaa gttcatcgca gccagaacga ttctgaacac gttagcgtcg
    15121 atggacgtga atatcataca tggcgtgaat tggcggatgc cttcgaactg gatattcatg
    15181 acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa aaaaatcccg accctgaggg
    15241 ggtcgggatg aaacttgctt aagcaagaag cacttaaaaa attcgttaca ccaggaaatc
    15301 tgatgtgttc atcaccttat ccgcaatttt tttcgctgac aagaaaatat tcgcgatatg
    15361 aataattaac gctacttatg aatagaaatg tgacgtgcgt caccccttgg tggctctggc
    15421 gtcaacggga tcgctcccga aaaaatcatc tttgattatt tgtgctgttt tgagaatcat
    15481 cctggtaagg ggagaactgc acgaccatgt tttcaattca acaaccacta ctggttttta
    15541 gcgatcttga tggcaccctg ctggacagtc atagttatga ctggcaaccg actgccctct
    15601 ggctcagccg tttacgcgaa gcgaatgttc ccgtcattct ctgtagcagt aaaacttcag
    15661 cggaaatgct gtacttgcaa aaaacgttgg ggctacaagg gttaccactg attgccgaaa
    15721 atggcgcagt gatccagctt gctgaacaat ggcaggatat agacggtttt ccacgcatca
    15781 tctcaggtat tagccatggc gaaatcagcc aggttttaaa tacgctacgc gagaaagaac
    15841 attttaaatt cacgactttc gatgatgtcg acgatgcaac catcgccgaa tggacgggat
    15901 taagccgtag ccaggcggcg ctgacgcagc tgcatgaggc gtcggtaacg ctaatctggc
    15961 gcgacagtga cgagcgtatg gcacaattta ccgctcgtct gaacgaactg ggcttacagt
    16021 ttatgcaggg tgcgcgcttc tggcacgtcc tggatgcctc tgccggaaaa gatcaggccg
    16081 ccaactggat tatcgcgacc tatcaacaat tatcaggcaa acgcccaacc acacttggcc
    16141 tgggcgatgg gccaaacgat gcgcccttac tggaggtaat ggattacgcg gtgattgtga
    16201 aagggctaaa ccgtgaaggg gtgcatctgc atgatgagga tccgacccgc gtctggcgaa
    16261 cgcagcgtga aggaccggaa ggctggcgtg aagggctgga ccattttttc tccgctcgtt
    16321 aagcgttatc gctcgcgcat acccgattac gcccggcctg tttagcgaga taaagccgac
    16381 ggtcagccag tgactggagt tgttcaaaat cataatcacc ggtttcctcg ctgctactta
    16441 cccccaacga ggcactgatg cgtatcgtcg tactcttagc aatcaacatc tctttttcat
    16501 ttaacttcag gcgaatacgt tctgcgactt ccgcagcctc cgtcagactc gcgcctggca
    16561 gaatcacaca aaactcctca ccaccgaccc gcccggcaac gtcctgcgca cgcaaggaac
    16621 tgctaattaa tccggcagca tgagaaagaa cacggtcgcc cgcctgatga ccaaagcggt
    16681 cattaatcgc tttaaaatgg tcaaggtcga cctgaatgac agaaaaagga tgttggtgcg
    16741 tctgacacaa tttcgcgagc ggacgggctt tttcaaacag tgcgccacga ttatataaac
    16801 gggttaaggt gtcgtgccac gcctgccact gcaacgagct ttgcagaaca tacatgttgc
    16861 taaccatccg gcgaatcaca taccaggaga tgagtaacat ggtggtaaag agcgcccaca
    16921 gcagggttaa tgcaatgctg atactgccga aatcgccgcg cacgccttcg cttagcgtat
    16981 ggacgcgcac cagcacaccg tcgaaatggt ccagacgttc ccagctaaca tagcgactgt
    17041 ccatacgaat gccgccccgc gtgtcatgtt ccatcgcctg cgccagcaag gccagttcac
    17101 gaggatcaaa aatattccct gttggatggt caggattgga agaggtcaaa aatctcagct
    17161 tactgtcata gagctgatac tcaccatcga ggtttttatc gatggcgttt cttaaaaatt
    17221 gctgcatggt acgcacggga atactcatcc ccagcacgcc ataccagtag ttattactgt
    17281 ctaccggaac actgacggta acctgcggtt cagtattgct ggcgtgttcc ggttgcgaag
    17341 taaaccagcg taccgcacgg tgacgatttt ctcgttgcga atggccgata aaccagggtt
    17401 gggtgacata gccgtaataa cgcgttggta cattgcgcgt aaacaaggtc ggctgcgtcg
    17461 aaacgtaaaa tccggcacgc gagacataca tcgcctgttc aaccatcgac gaggtgttgt
    17521 gcgccagtcg cagcaagtaa ccaacttcca gtgcagcggt aatttcattg tcgaggcttt
    17581 cattttcgcg agacaggaga ttcccctcgc tgactaatgc atccgatacg ccattgactg
    17641 acagggtgcg tcgtcggttg agttcaattt gccaggcgtg ctcgtcgcga tgctgttcga
    17701 actcggttac agcattacgc agtgaagtga aatccagtgg cgctacgaga gcttcgcgca
    17761 tgccattacg caaaaagatc agtttgtcga cgttatactg caaatgctta tcgagcgcgt
    17821 tggctacgtt ttccagatga ttacgctggc tggagatata ggcatcttcc agcactacca
    17881 cttcacgcca ggtcagcaag gtggaaaaaa gcaataccac gataaagcag agattaacga
    17941 catgaccagg ccccaggcgg cgtgcgagtt ttttcaacca gctctggttt tccatttttg
    18001 tctcgtgctg caccctgacg actcccaacc tgctttttta tgattctggc ataacctggc
    18061 gatagcgtac cgcaaaacag cgataatttt ataatggtgg catgataaga cgcgactatc
    18121 attcattgca ggcattggca caccaggccg gaagtgatcc ggcctggtgt taatgatacc
    18181 ctttcaaact tcttccgcag aacgactccc ctcccccgga acaagctcgg tttcatgaaa
    18241 agcttcccgc ttgaccccgt aaccgtcata ccaacgacac tcaaccatac cgctggagta
    18301 tccagtgaca atcatccgcg ggccgccctc tttaaccgta acttcctcac taaccataaa
    18361 gctcatactc gcctcctttt ttcgagtgaa acttgttcac cttagttgaa gatggcgaat
    18421 tttgcataat agcctgtacg agtattaatg tgcctgaccg cgcattttcg ctacggcttt
    18481 cactcccagc accacgatgc cgccgatgat aaatccaaga atcagattta aaacagtcgg
    18541 taatatcatc gccaccactg cactttgctg cccggcgaaa tgttcaatgg cgtgatgcag
    18601 cggcgcaata ccatgtacca caataccgcc gccgacgagg aacatcgcca gcgtgccgac
    18661 aatcgataac gccttcatca accagggcgc aatgatcaat aaccctttac ctaatgcctg
    18721 catcagcgcg ctggattttt ccgccagcca ataccccagg tcatcaatct taacgataac
    18781 ccctaccaga ccgtagacgc ccacggtcac taccagcgcg atgcctgaaa gcaccagcac
    18841 ctgattaagc aacggcgctt cggccacaat ccccagcgtg atggcgacga tttcggcaga
    18901 caatataaaa tcggtacgaa tcgccccttt tattttgtcc ttttcaaact tcagcggatc
    18961 ctgcgccgcc agcttctcca gacgctgctg gctctgcgcc ggatcttctt tatgtttacg
    19021 cgcctccagc atatgcagca ctttctctac tccttcaaag cagagaaacg cgccaccaat
    19081 catcaacaga ggcgtaatcg cccacgggat aaacgcactg atgatcagcg ccagcggcac
    19141 cagaatcact ttattaatca gcgatccttt cgccacgccc cagaccacgg gaagttcccg
    19201 gttggcccgc acgcctgaaa cttgttgcgc attgagcgat aagtcatccc ctaatacacc
    19261 ggcggttttc ttcgccgcca gtttgcccat cacggagata tcgtccagta gtgtggcgat
    19321 atcatcgagc aacgtcagta aactactccc ggccaaaagc attccctctc atttttattg
    19381 ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat acttgcttca caggtcaaca
    19441 ttttttttac aatcaagtgc aattatatct ctcgccagca gaatgatttt cacgtttact
    19501 atgaaccacc tttttatttc caccgtgagg gattatgcgt ttccgccagt tgttaccgct
    19561 ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca acctattttg tcattcggat
    19621 tggcgtggaa agctggcctc cgttaatgat ggcaggcgtt cgattcctgg cagccggtat
    19681 tttattgatg gcatttttgc tactgcgcgg acacaagctc cccccgctac gtccgctgct
    19741 caatgccgcg ctgattggcc tgttattgct ggctgtcggt aatggcatgg tgacggttgc
    19801 cgagcatcaa aatgttcctt ccggcatcgc cgccgttgtg gttgcaaccg tgcccctctt
    19861 taccctggtg cttcagccgc ctgtttggca ttaaaacgcg caaactggaa tgggtgggta
    19921 tcgccattgg gcttgccgga atcatcatgc tcaatagcgg tggaaattta agcggcaatc
    19981 cgtggggcgc gattctgatt ttaatcggct cgattagctg ggcgtttggc tcggtttatg
    20041 gctcgcgcat taccttacct gtagggatga tggcgggtgc gattgagatg ctggcggcag
    20101 gcgtggtgtt aatgatcgcg tcgatgattg caggtgaaaa actgacggcg ctcccttccc
    20161 tttcaggctt ccttgcggtc ggctatctgg cgctgtttgg ttcgattatc gccatcaacg
    20221 cttatatgta tttaatccgt aatgtcagtc cggctctcgc caccagctac gcctacgtta
    20281 acccggtggt cgcggtcttg ctgggtacgg gactgggcgg agagacgctg tcgaagattg
    20341 aatggctggc gctcggcgta attgtcttcg cggtggtact ggtcacgttg ggaaaatatc
    20401 tcttcccggc aaaacccgta gttgcgccag ttattcagga cgcatcaagc gagtaaatga
    20461 atcccctgcg tgtcgatctg cgcgctggca ccttcgccgc agatccactc ttccagacgc
    20521 tcggtaagcg cttcatccgt cagcttctcg cgcccacgta atgcacactc ccagacaatc
    20581 agtacgcgcc agccgagttc ctgcaaacga ctgatatcgc ggcgatcgcg ttcaacattt
    20641 ttacctatct tctccagcca gaactcggtt cgagtcgcag gcactttaaa cagatagcag
    20701 tgatgatgat gccagaagca gccatgggta aatatcacgc agcgatattc atcaacgaca
    20761 aaatccgggc ttccgggcag actggcgtcc tgaacgcgaa acgccaggcc ctgcccggtt
    20821 aacagactgg cgaggcgctt ctcaatcgcc gtatcacgcg tggcaatcgc gcgcatattt
    20881 ttgctgcgag tggccttatc gtgaacgtcg gccatgttgt gcctcttgct gacgcaacgc
    20941 caccgcctgt ttgatttttg gctcaagcag ttttgccacc gcggcaaaga ccggcacgac
    21001 caccgagtta ccgaactggc gataggcctg agtgtccgaa accggaatgc ggaatttcgc
    21061 ttctcccggc gcttcaaaac ccattaagcg cgcgcattcc cgaggcgtta accgacgtgg
    21121 acgatgttgc tgattcagcg gatcgtcaaa atctttctca cccgtggcca tatcccagcc
    21181 gcgatcgatt aaaatttccg cgccatcttt gtaataacgc gcagacagcg tacgcgtgac
    21241 gctttgcgga ttgttcggat aaaccattcc ataaccgaag ccgttaccgc gcgcctgatg
    21301 ttttttcgca tatcgataga ggtacttcca cagcaccggc gtcaggatat atttcgcctc
    21361 gaccatcggg tccaacagct gcgccagcgt cactcgctgt gcagggaaac attcgctgat
    21421 atcacgcaga gtaaaatcgg ctttcagatt aagatcgcga cgaaaaccca ccagcacgat
    21481 gcgttcacgg tgctgcggca gaaaatgttt accatcgatg attttcggat cgtccggccc
    21541 gttatcttct gcatcagcca cgtcatagcc cagttcgtcc agcgtctgca tgatgatgcg
    21601 gaacgtttta ccctggtcgt gacttttcag gtttttgacg ttttcgagca caaacatcgc
    21661 cggacgacgc gcgtcgataa tgcgtaccac atcaaaaaac agcgtgccct gggtatcgca
    21721 ggcaaaaccg tgcgcccgcc cgagcgagtt ctttttcgat acgccagcca gcgaaaatgg
    21781 ctgacaaggg aaaccggcca gtaaaacatc gtgttcagga atgtgttgac gaatatgttc
    21841 cgccgccgcc tcatcactca cgccttcttt atggctgagg gtgatgtcgc ggatatcttc
    21901 attaaaatga tgcgtcgccg gatcgcaata atggttggct ttataagtgc gtaccgcatg
    21961 tttgttccat tcgctggtaa acacgcattg tccgccaatc gattcaaaac cgcgacggat
    22021 gccgccaatt ccggcaaata gatcgataaa gcgaaacgca taatgcggat gatgtgccga
    22081 tggtttgggt aacaacgttt gcagatgggc gaactctttc tcacttaaac ggtgccatgc
    22141 cgagtcattc gccagcgcac gttttaaaat tgccgcgctc cagtgattct cacccacacc
    22201 attaagctgc gccaccagcg ttttaacatc ataaatttgc agcagtttct ccagcattgc
    22261 ctgtgcggca ttcccggtgc tgtatgaatc ggttactgat atattttcct gcatagattt
    22321 aaccggccat ctgagatggc cgacaggtta acataattac agccatttag ggccacgcgc
    22381 ttatcccgcg ggtgaaaacg catctatcac cttgtgatcg acaccttcat tctcccccgc
    22441 cagttcggca ctgagcttcg ccataaactc gactaaaaaa tgcgcgttgt gctgcgccag
    22501 ctgcttgcct cttgcggttt gcatggtttg cggtagcttc agcaacttag tctggaaatg
    22561 atccagcgcg tagcgtttat catcaagcgg gcgatgctgt gcgaacggat cttcaccatc
    22621 aaacagcgcc acgcccaatg cccctgaaac ggcaaacacg cgcgccaggc caatcgcccc
    22681 cagggcttcc agccgatccg catcctggac gattttagct tccgttgtta agggggcgat
    22741 ttgcgcgctg aaactgtgag cggcaatggc atgacaaacg gcctcgattt tctccgccgg
    22801 aaattgctcg aactcttcac ggagcaggcg acgcgtctct tctgccgcca ggattgaaga
    22861 acgctgcctt tgcggatgat ttttcgccag gctcacaata tcgtgaaaat aacaggcggt
    22921 taaaatcacc agcatatcga catcgtcatc tgccgccagc ttctgtgccg cgtcctggtg
    22981 ctgatgatgg tttttcaacc agttttcgaa ctgtgcctgc cagtgttgta agtccatacg
    23041 cgctccgttg ccaaagagtc gccaccttag cagtttttat tcatagcgtc tgctacgtgc
    23101 gggacgaggt cgggtgtacc aggcaatgct gcctaacaat gccccgacca gagccataaa
    23161 taaaaatccg accagtgccc ctaaccactt tcccgcggta gagcctaaat cgctttcaaa
    23221 tcccgacacc ggggcatcag ggatccggct catcacccag atgtaacgcg ccattgccca
    23281 gacgaaatag ccacaccatg tatagaaagc ccacaaagcc aatttgccgc cagggctgcg
    23341 agagagtctc tcttccatct ttgtcgaacc gtattccatt ttgagataac ccggaaaatt
    23401 cagacttagt actattatgt gataaaagtc acatttttcc acattggatg agccagtcaa
    23461 gactcaatca ttttttgaac aaatagataa attttgcgca acggcaataa ttactggaaa
    23521 aacagcaata aagatttatt tatatccttg gtaatagttt tattaattca cccaaacaat
    23581 atattagcac tgaatacaat tatacctttt ttgatttctg tgtcatgata accccgttat
    23641 tagcctttta tcgtcttgtt tatatttttt gggccggcat gatgccggct ttttttatac
    23701 cttcattaat gtgcgcgtga tcacgcctgc cgtttggcgc aacaatcatt gatactccct
    23761 atctttccat t
//
LOCUS       DAZPAC010000050        23491 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZPAC010000050 DAZPAC010000000
VERSION     DAZPAC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.050"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..693
                     /locus_tag="ACHUPM_001639"
     CDS             <1..693
                     /locus_tag="ACHUPM_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0869450.1"
                     /translation="QKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSSWGIDD
                     RDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFVASTAE
                     CCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQYFAGY
                     TFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQPLADYP
                     TCPETLKDMSDL"
     gene            690..2117
                     /locus_tag="ACHUPM_001640"
     CDS             690..2117
                     /locus_tag="ACHUPM_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR0869451.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2127..2681)
                     /locus_tag="ACHUPM_001641"
     CDS             complement(2127..2681)
                     /locus_tag="ACHUPM_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0869452.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            2783..3490
                     /locus_tag="ACHUPM_001642"
     CDS             2783..3490
                     /locus_tag="ACHUPM_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0869453.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            3487..4941
                     /gene="djlC"
                     /locus_tag="ACHUPM_001643"
     CDS             3487..4941
                     /gene="djlC"
                     /locus_tag="ACHUPM_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR0869454.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDDEEEREAFLEA
                     IQAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAK
                     LHRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLC
                     AYWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTR
                     LLWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVV
                     LEAFPKQEMLARLNTRLEGGDTHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKN
                     DEDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(5001..6671)
                     /gene="hscC"
                     /locus_tag="ACHUPM_001644"
     CDS             complement(5001..6671)
                     /gene="hscC"
                     /locus_tag="ACHUPM_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFR0869455.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(6763..7698)
                     /gene="rihA"
                     /locus_tag="ACHUPM_001645"
     CDS             complement(6763..7698)
                     /gene="rihA"
                     /locus_tag="ACHUPM_001645"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR0869456.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(7816..8541)
                     /gene="gltL"
                     /locus_tag="ACHUPM_001646"
     CDS             complement(7816..8541)
                     /gene="gltL"
                     /locus_tag="ACHUPM_001646"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR0869457.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(8541..9215)
                     /gene="gltK"
                     /locus_tag="ACHUPM_001647"
     CDS             complement(8541..9215)
                     /gene="gltK"
                     /locus_tag="ACHUPM_001647"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR0869458.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(9215..9955)
                     /gene="gltJ"
                     /locus_tag="ACHUPM_001648"
     CDS             complement(9215..9955)
                     /gene="gltJ"
                     /locus_tag="ACHUPM_001648"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR0869459.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(10125..11033)
                     /gene="gltI"
                     /locus_tag="ACHUPM_001649"
     CDS             complement(10125..11033)
                     /gene="gltI"
                     /locus_tag="ACHUPM_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR0869460.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            11488..13365
                     /locus_tag="ACHUPM_001650"
     CDS             11488..13365
                     /locus_tag="ACHUPM_001650"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR0869461.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(13443..14981)
                     /gene="lnt"
                     /locus_tag="ACHUPM_001651"
     CDS             complement(13443..14981)
                     /gene="lnt"
                     /locus_tag="ACHUPM_001651"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR0869462.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(15006..15884)
                     /gene="corC"
                     /locus_tag="ACHUPM_001652"
     CDS             complement(15006..15884)
                     /gene="corC"
                     /locus_tag="ACHUPM_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR0869463.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(15974..16441)
                     /gene="ybeY"
                     /locus_tag="ACHUPM_001653"
     CDS             complement(15974..16441)
                     /gene="ybeY"
                     /locus_tag="ACHUPM_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR0869464.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(16438..17478)
                     /locus_tag="ACHUPM_001654"
     CDS             complement(16438..17478)
                     /locus_tag="ACHUPM_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR0869465.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(17631..19055)
                     /gene="miaB"
                     /locus_tag="ACHUPM_001655"
     CDS             complement(17631..19055)
                     /gene="miaB"
                     /locus_tag="ACHUPM_001655"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR0869466.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            19201..20376
                     /gene="ubiF"
                     /locus_tag="ACHUPM_001656"
     CDS             19201..20376
                     /gene="ubiF"
                     /locus_tag="ACHUPM_001656"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR0869467.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(20530..20604)
                     /locus_tag="ACHUPM_001657"
     tRNA            complement(20530..20604)
                     /locus_tag="ACHUPM_001657"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20570..20572),aa:Gln,seq:ctg)
     gene            complement(20641..20715)
                     /locus_tag="ACHUPM_001658"
     tRNA            complement(20641..20715)
                     /locus_tag="ACHUPM_001658"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20681..20683),aa:Gln,seq:ctg)
     gene            complement(20764..20840)
                     /locus_tag="ACHUPM_001659"
     tRNA            complement(20764..20840)
                     /locus_tag="ACHUPM_001659"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20804..20806),aa:Met,seq:cat)
     gene            complement(20856..20930)
                     /locus_tag="ACHUPM_001660"
     tRNA            complement(20856..20930)
                     /locus_tag="ACHUPM_001660"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20896..20898),aa:Gln,seq:ttg)
     gene            complement(20965..21039)
                     /locus_tag="ACHUPM_001661"
     tRNA            complement(20965..21039)
                     /locus_tag="ACHUPM_001661"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21005..21007),aa:Gln,seq:ttg)
     gene            complement(21063..21147)
                     /locus_tag="ACHUPM_001662"
     tRNA            complement(21063..21147)
                     /locus_tag="ACHUPM_001662"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21111..21113),aa:Leu,seq:tag)
     gene            complement(21156..21232)
                     /locus_tag="ACHUPM_001663"
     tRNA            complement(21156..21232)
                     /locus_tag="ACHUPM_001663"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21196..21198),aa:Met,seq:cat)
     gene            complement(21612..23276)
                     /gene="asnB"
                     /locus_tag="ACHUPM_001664"
     CDS             complement(21612..23276)
                     /gene="asnB"
                     /locus_tag="ACHUPM_001664"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR0869468.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(23230..>23491)
                     /locus_tag="ACHUPM_001665"
     CDS             complement(23230..>23491)
                     /locus_tag="ACHUPM_001665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869469.1"
                     /translation="PVKYNHSIKPAKNYRFINKYILTPDPFSPIDNHHYVFYFFTAKS
                     LAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
BASE COUNT         5738 a         5818 c         6031 g         5904 t
ORIGIN      
        1 caaaaaatat tgtttgcact ttccactccg atggaaatac gcaatgaatg ctgtttacct
       61 tcgcattcat cccccaaaat gtatttaggt acatgctttt ttgacctttc ttcctcatgg
      121 ggaattgatg accgtgatga cctgctgcgc acaattcacc gaatgatcga taacggtcat
      181 gccgctcggt tggcagggtt ttatcaccgc tggtttcgtt attcgccatg tgaatggcgt
      241 gactatcttg ctgaactaaa tgagcaaggt caggcttatg cgcaatttgt cgccagcaca
      301 gccgaatgct gtggtgaggg aggaattaaa gcatgggatt atgtccggat gggtttttta
      361 agtcgaatgg gcgtacttaa taactggttg agtgaagaag aaagtctatg gatccagtcg
      421 cgtattcatc tacgggctct acgttattat agtaactggc ggcaatactt tgccggttat
      481 acctttggtc ggcaatattg gcagtctccc gaagatgatc atctgccttt attacgcgaa
      541 ttcttagctc gtaaagaata cgacgattcc ggcaatgata tgttttatca attatttgcc
      601 agtgatgatg cgtattaccc tgccttgtcc tggcaaccac tggctgacta ccccacatgc
      661 ccggaaacgc ttaaggatat gagcgaccta tgaaaaattg ctggaagatc ctcgaaatag
      721 aggaaacgac tgacgtcgat attatccgcc gcgcttatct ggcgctgtta ctgtcctttc
      781 atccagaaac cgatccgcag ggttttaaac aacttcgtca ggcgtatgag gaggcgctcc
      841 ggattgcgca gtcgcctgct aaatctgttt ggcaaccaga agaatatgag gtagcagaac
      901 atgaaattct gctcgccttt cgcgcgttac ttgcctctga tagcgaacgt tttctgccct
      961 ccgcctggca gcgattcatt cagcaattaa attattgctc gatggaagag attgatgaat
     1021 tacgctggtc gctgtgcaca atcgccatga acactgccca tttatccttc gagtgcgtgg
     1081 tgttattagc agaaagattg cggtggttgc aggaggaaaa cgtcggggaa atagacgaag
     1141 aagaactgga atccttttta tatgccattg cgaaggggaa tgtttttaac ttccagacca
     1201 ttctgcatct gcccgttgcc gtgcaaaatg acaccatcga tttttaccaa atgttcgctc
     1261 ggatttggtc atcgcatcca gaatggctga cattgtattt agcgcaacat cgcgcagtga
     1321 tcatccccga tgatgcaaaa ctgcacagaa atttactccg ctggtatagc gcaggtcgcc
     1381 tggatatccc cgaacttctg gattacgccc ggtcgtggcg ggaagctgaa cctgataatg
     1441 aagatgcgcg ttattatgaa tacgcgcaac gcgtctattg tggagaaggc gaaagcttgc
     1501 tggcagaact ttgtgactac tggcgcgagt atccctccac ccaggcggat gctttaatat
     1561 tgcaatggtg ccgtcagcat cgggtcgatt attacccgtt agtggtgatg atgattgaag
     1621 ctcgtgaact ggttaacgat cagggtaaac agctacttta tgtccccggc gacagcgccc
     1681 gtacgcgttt tcatttatac gaaatactca gcgatgaaaa actctctgcg ctggggcgtt
     1741 cactggtcga gatggttttg cacaaaggac gtaagccgcg gatctcactc acgcgtgata
     1801 cagaacatcc cttatggcca ttatatttag ttgccaaaca attagtgcag gccagccaac
     1861 cgacagaaga atcattaatg ccgattgtga gccgccttga tgcagaagat cgttgtccac
     1921 tggaagcatt aattattcgt cgattattaa ttcaggcggc gaattttacc ggtcaggaaa
     1981 ccgtcgagcc ggagccgcaa ccgcagccaa tgcccgttga cgatggcggg ctgggctgtc
     2041 tgggcgtcat caaaattatt ttctacattt ttatctttgc cggtttgata ggaaaaatac
     2101 tccatctctt cgggtgacag tcgacgttaa tgtgcgggaa gtccggcaag taatctttcc
     2161 gcatttttgt aatgttgttt ggcggccagt tttaaccatt tttgagattc ataatggtta
     2221 acattaatac cagtgccatt tctgtagatc aaacccaaag tatattgagc atgagcatac
     2281 ccttgttcag ctgcttcctt ataccagaga attgctttac gggtatcggg tttgactccc
     2341 aggcctttca ggtagatcca ccccagtttg ctttgtgcgc gcggattttc ctgttctgct
     2401 gattgctcat accagtatct cgcctgtgta taatccagtg gtttaccgtc gctaccatga
     2461 agataaaatt cgccaaggcg atattgcgat gttgagtgac catgaatcgc ggaaagctcc
     2521 gtggctgccg tggttaacgg tagactgcct ggtgatctta aataatcaaa ggcataattc
     2581 accatggctt tatcacgcaa cgtcgttttc ttgggttggc taatacaggt aattgctgca
     2641 actataaaaa ttgcgaatat atacattgat tttttattca tgtgtgaatg cttcgatttt
     2701 ctttttattt tgaaagcgtc aagtttatac tcatcaataa taaaaaagta gtcagttaat
     2761 gcacttaagg gattattaaa aaatgaataa ggaagaacaa tatcttcttt ttgcgctttc
     2821 tgcgcccatg gaaatactta atcagagttg caaacccgcg catgactcgc caaaaatgta
     2881 tactgggata aaagaattcg atctctcatc atcctgggga ataaataatc gtgatgattt
     2941 aattcagact atttatcaga tgactgatga tggtcatgcc aatgatttag ctggcttata
     3001 tctgacatgg caacgctctt cccctgaaga gaggaaagca ttaattgctg gtggctcgga
     3061 gagagggttg atttataccc aattcgttgc ccaaaccgct atgtgctgtg gagaaggggg
     3121 aatcaaagcc tgggattatg ttcgaatggg gtttctgtca cgggtcggtg tgctcaacaa
     3181 ctggctgacg gaagaagaga gtttatggct gcaatcgcgc gtctatgcac gagctcacca
     3241 ttactatcac agctggatgc actatttttc tgcctattca ctagggcgtc tctactggca
     3301 atcttctcag tgcgaagaca acgcttcgct acgcgaggcg ctaaccctgt acaaatacga
     3361 tagcgccggg agtcggatgt tcgaagagct ggcagcggga agcgaccgtt tctatgccac
     3421 gcttccctgg caaccattaa ctgtccagcc tgaatgcccg gtaacgctta aggatgtgag
     3481 cgacctatga aaacatgttg gcaaatcctc gaaattgaaa gcacgacgca aatagacatt
     3541 atccgccagg cttatcttgc tcgcttaccg ttgtgtcatc ccgaaaccga tccgcagggg
     3601 tttaaagcat tacgccaggc ctatgaagag gcgctgcgac tggcggtaaa tcctgtcgag
     3661 gaagccgatg atgaagaaaa agatgccacc gctgaacatg aaatactacg tgcattcagg
     3721 acattactgg attcagaaag tgatcgtttt cagccctccg cctggcagaa atttattcag
     3781 caattaaata cctggaacat ggaggatgtc gatcaattac gctggccgct gtgtgcaatc
     3841 gctatagaag cgcgatatct ttcattaaat tgtgcttctt tgttggcaga gcgtttgaac
     3901 tggcattcgt ttaatgacag cgaaggaatg gatgatgagg aagaaaggga ggcttttctt
     3961 gaggccattc aggctggtga ttgtttcgat ttccttagcc ttctggaata tcccgtcgcg
     4021 ttacagaacc agactgttga atattacttc gcgctggaac gttgctgccg ttaccatcct
     4081 gactatgtca ctgcgttttt ggcgatggaa ggaccgtggt tcattcctga tgatgcaaag
     4141 ttacatcgca aactgttgcg ctggtacagc tcggtgcaaa caggtatggc ggaactcatt
     4201 cctgtagcca aacagtggca agcggaagaa ccagaaagcg aagatgcccg gtattaccag
     4261 tgtgcacaac gtttgtactg cggcgagggg gaaagcctgc ttgccgatct ctgcgcgtac
     4321 tgggaaagtt acccatctac acaagctgat aatttgttgt tgcagtggag caagcgtcat
     4381 tgcccggatt atttcgcgtt attagtgatg gttatcgaag cgcgaagcat ggtagatgcg
     4441 caaggtaaac cgttgaaata tgttcctggt gagagcgccc ggacgcggct gttatgggcg
     4501 gagattttac atagcggaaa attatcgccg ttaggtcaat cgtttattga gtcgttattc
     4561 ttcaagcgca aagcatgggc gtggtggaaa tcgagagtcg gtagcgagac agagcaagat
     4621 tcaccgttac tggatttgta tcgggtagcg gaacaggttg tgctggaagc gttcccgaaa
     4681 caagagatgc tggcccgtct taatacaagg ctggaaggtg gagatactca tccattagag
     4741 gccattatca cccagatgct gttgacgaaa gtgaaactcg agccggagga tgaagatgtc
     4801 gatgagccaa cacctgaaaa ccatgaagaa aaaaatgatg aggatgaaaa accacagagc
     4861 attaccagta ttatcaaaat cagcttaacg gtgctgatga taggttatgc gctcggaaaa
     4921 atcgcgatgt tgtttagctg aggatgtgtt ttttgcgccg atgccggatg cagcgtttat
     4981 gccgcatccg gcagccgtgt ttaagggatc tcaatagcca aataatcgct cgcccgtcga
     5041 cgaacttcat cgacgcgggc catatcgttt gactgcaacg ccgcatcaaa atcggtgatc
     5101 acccggccaa tctcttctcg ctcgtcaccc agcgcccgcg cccacaactc ttccagtttg
     5161 gccttaaagg tgcggttgat tagcatatcg cgcggataaa ttttcaatgc ggataaccgc
     5221 gtccgactct cttcaatctg ctgcgcgctc aatgttaccg ggctatgatt aatgactctg
     5281 gacttaacgc tgccatcttc cagaagcacg tcaacttcaa gcaagccgtt gatatcataa
     5341 ctaaagcgaa tatcaatcga ctgataagcc ccggttttct tcaacggcac atcgaaggat
     5401 tcaaccagaa tgttattttt aactttgtgg ttttctccct gatagacgtt aaccgtaata
     5461 gaatcctgtt ccgggtgcat ggtcgagtaa gtttctacac gcgacacggg tactgtggtg
     5521 tttcgttcaa taattggcga gaaaatgccg gaaacgccct ggcggttaac ttcaacgccc
     5581 aacgagtaag ggcaaatatc agtgaggatc acctcttcaa tatcttcact gcgtaagcgg
     5641 caggcggcct ggattgctgc gcccagcgcg acaatggtgc tcgggtcgta actttgatac
     5701 ggtaatttgc caaacagacg cacggcgatt cgctgcacca gcggcatttg tgacgcgccg
     5761 ccaaccagca ccagactgtc gatttgactc ggcttcagac gtgcatcgcg caacgcctgt
     5821 tcaatcggca ctcgcaagcg attgagcagc ggcaaccaca aatcttccag ttcgttctcg
     5881 taaaattcgc actcccgcgt ttcatcctgg tactgccagc gaatgtgcaa tggcgattga
     5941 ttgctacatt ttgccgcttc cacacaggcg tacaaggctg ccagttcact ctcgttaagc
     6001 gtggtcctgg cgacatccgc gcgctttaaa acgtcatcga ccaacatatg ggtaaaatct
     6061 tcgccaccga ggaagttatc gccagcggag gcgtgaactt caatcaccgg cgtggcgtat
     6121 tcgagcaccg tgacgtcaaa cgtgccgcca ccgagatcaa aaaccagcga gcgggtattt
     6181 tgttgcgtat gcaggccata tgccatcgca gctgctgtgg gttcattaat taagcgtacc
     6241 gcatttaacc cggctaactc cgctgctaaa cgggtatgct tgcgttgttc atcgctgaaa
     6301 taagccggaa cggagatcac cacatcttta atcggacgtt gcagaaattc ttcggcatct
     6361 tcttttaatg agcgtaatac caaagaggac agttctggcg cgttaaatgt gtcgctgcct
     6421 aaccgccagt tggtattact gcccattgca cgtttaaata acgctgccgt tttatccgga
     6481 tgcgaagtac gccgtgatac agccggtttt ccgactaaaa tatgattatt ttcatccatg
     6541 ctaattatgg atggtgttaa atattcaccg aacttatttg gaattaattg cgcggcaccg
     6601 tctttccaga cggcaattaa actattggta gtgccgagat cgataccaat ggcgagttct
     6661 gcattatcca ttgcatttat atcttgaaag aaaaagagat aaacagataa acagattaag
     6721 accacccagg taacgcgtgg tgatcttaat caatgacgtg tgttaagcgt aaaatttcag
     6781 acgatcagcc agtaaatcaa caaagccctg acgatcaaca tcgaccatta cagtggcatt
     6841 cggtttattg ccagtcagat aataataatc aacaaccgtc ataccctggc tatatttccc
     6901 ctgtgtttcc acgccaaccc agcgctcaac agtggtaaac aactcgggtt tcaacagcca
     6961 ggcgatggtg catgggtcat gcagcggtgc accgacaaag ccccattttt cgtctttatg
     7021 atattcgagg aagaaatcca gcagttcggc aacaatggtt gaaacagggt tgccaatcgc
     7081 gcggaaacgc tcggtgtctt caacgtggat ttgtgctttg tgagtaacat ccagaccggc
     7141 catcaccact gggatccctg actggaagac aatttctgcc gcttccgggt caacgtaaat
     7201 attaaattca gccgcaggcg tccagttacc aagccccatt gcgccgccca taatcacgat
     7261 acgggcaatt ttgctatgca gttccggatg gctattgagc agcaaggcaa cgttagtttg
     7321 cggtccggta gacacaatcg tgacaggttc cgcactttcg cgcagcgttt tcgccatcag
     7381 ctctaccgcc gtacagtttt gcggtgcgaa cgtcggttcc ggtaatgccg ggccatccag
     7441 accgctttcg ccgtgcacat tgtccgcgat aatcaactca cgcattaacg gttttaccgc
     7501 gccgcctgct accggaatat cggtgcgatt aagcaaggtc agcatacgca gaacattgcg
     7561 taaggttttt tctggtgtct ggtttccggc ggaagacgta attgctttga catcaagctc
     7621 tggtgaggcg agggcgagaa ctattgcgat agcgtcgtca tgacctgggt cgcaatctaa
     7681 cagaattggc agtgccattg ttgctccttg ttgtgtgctt ctttgcgaca agggtaacgc
     7741 caggatgtaa cagatacgag gggcgaaacg ataaagcgtg agatggcgcg cacttgggta
     7801 tgcgcgccag agtgattaat gcaggatttt cgcgaggaag tctttcgcgc ggtccgattt
     7861 cggatcatcg aagaaagcgt ctttcggcga gtcttcgaca attttaccct cgtccataaa
     7921 gatcacccga ttcgccactt tgcgggcaaa gcccatttcg tgggtcacca ccatcatggt
     7981 cattccttcg ttcgccagtt ccaccatcac gtccagcact tcgttgatca tctccggatc
     8041 cagcgccgat gtcggttcgt cgaacagcat cgcaatagga tccatacaca atgcgcgagc
     8101 gattgccaca cgctgctgct gaccgccgga aagctgcgcc ggaaatttat tggcatgtgc
     8161 ggaaagtccc acgcgctcca gcaatttcaa tgctttttca cgagcgggcg ctttatcgcg
     8221 tttaagcact ttcacctgcg ccagggtcag gttttcgata atcgacagat gagggaacag
     8281 ctcgaaatgc tggaatacca tcccgacgcg ggaacgcagc tttgccagat cggttttctt
     8341 gtcattaacc acgataccat cgacggtgat ttcaccttgc tgcactggtt cgaggccgtt
     8401 gacggtttta atcagcgttg atttgccgga accagacggg ccgcaaacca ccaccacttc
     8461 gccttttttc acttcggttg agcagtcggt cagcacctga aagtgaccat accattttga
     8521 aacatttttc agggtaatca ttatgctgtc cttcttttca agtagctgac caacaacgac
     8581 gcgctaagac taataacgaa ataaacaaat ccggcaaaca ggatcatctc aacctgcgta
     8641 ccatcacgct caccaatggt tgaggcggta cggaagaaat cggccagaga taacacatac
     8701 accagtgagg tatcctggaa cagtacgatg ccctgagtga gcagaagcgg caccatcgcg
     8761 cggaacgcct gcggcagaat aatcagtttc atcgactgcc agtgagtcat tcccaacgcc
     8821 agcgcggcgc tcgactgacc acgagaaata ctttgaatac cagcacggat aatctctgaa
     8881 tagtaggccg cttcaaacat cgaaaacgcc accatcgccg aaattaaacg gatatcattt
     8941 tttggtgata atcccagcac gttttgcaga aaacctggca cgatcaggta aaaccacagc
     9001 aaaaccataa ctaaaggaat cgagcggaat acgttaacgt aggctttggc aaaccacgct
     9061 acgggcgcaa agctggacaa gcgcatcacc gccagcatgg tgccccacaa aataccaatc
     9121 acgactgccg tgacggtgat tttcagcgtg atcaccagcc cgtcgagcag atatggcagg
     9181 gaagggacaa tggaactcca gtcaaactcg tacattattt gccccccatg ttgccaggca
     9241 ggcgaacttt acgttcaacc agcgtcatta ccagcatgat aaccgcgtta atcaacacat
     9301 acgccagcgt aatagcggta aacgactccc aggcgtgggc tgagtaatca agcaatttac
     9361 ccgcctgcgc cgccatatcc accagaccaa tagtcgaggc gatggcggag tttttcacca
     9421 ggttcatcat ctcggaagtc atcggcggga cgataacgcg ataagcatta ggcagcagta
     9481 cgtatcgata agcctgcggt agcgtcaggc ccatcgccag cgcggcattt ttttgcccac
     9541 gcggcaacga ctgaatggcg gcgcgtacct gttcgcaaac acgggcggcg gtaaacagcc
     9601 ccaggcagag catggatgaa aggaaaaact gaatattggg atccagctcg gccttaaacc
     9661 acatgccgat tttctccggc aacagctccg ggatcaccag ataccaggta aagaattgca
     9721 caatcaacgg cacgttgcgg aacagttcga catacaacgt acccagaccg gagagaaaac
     9781 ggtttggaac ggtacgtaaa atgccgaaaa atgaaccgac gaggaaagcg ataatccagg
     9841 cgcagatcga caaagcgatc gtcacctgaa aaccactcca gatccaacca agataggtgg
     9901 tgttgccgaa cggggcttgt tgtaaaaaaa taccccagtt ccagtctata gacataaatc
     9961 tactccagaa aaaagagggt agcagcgtta actgctaccc tcgaagattg ttacccagcg
    10021 tattgcggtt ttcaggcccg atggggaacg accatcaggc gtatagtctg tccgtgctac
    10081 gtaacaatcg agagggctgg aatttccgcc cctggttctt gtaattagtt cagtgccttg
    10141 tcattcggtt ctttgaacag tgctttcatt tcgtctgaca gttcgaaatt catgttcagg
    10201 tttttcggcg gaatgggatt tttgaaccat ttatcaaacc atttttccgc ttcaccggag
    10261 gtctgcacct gcgcggtggt gtcatccatc agctttttga actgcggatc gtctttacgc
    10321 aacatgcaac cgtaggcttc ctgagactgc ggcttgccga cgatttccca gttgtctggt
    10381 ttcttcgctt tcgcacgttc accggccagc agggcatcat ccatcataaa ggcaacggca
    10441 cgaccgcttt ccagggtgcg gaaagagtca ctgtgatctt tggcgctgat gatgcgcata
    10501 ttcatttttt gctcttcatt cagtttgttg agcaaaactt cagaggtagt gccggaagtg
    10561 acgactacgg ctttgtcttt caggtcggca aaatctttga tatcgccgcc ctttttggtc
    10621 agcaggcgcg taccgaccac gaaaatagta tcagagaaag ccgcctgttt ttggcgttcg
    10681 acgttgttgg tggtagaacc acattcaaaa tcgaaagtgc cgttttgcag cagtggaata
    10741 cggttttgtg aggtaatcgg aatcagtttt acctgcaagt ccggtttgtt gagtttcttt
    10801 ttcactgctt caacaatggc gttggagtaa tcctgcgagt aacccaccac tttttgctga
    10861 ttgtcgtaat aagagaaagg tactgaagat tcacggtgac cgacgacaat cacaccgttt
    10921 ttggcgattt tgtccagcgt actgcctgct gccggggcgg cgtcatctgc ctgtgccagt
    10981 cctgcggaaa gcgccagggc gaggattgct gtggcaggtt tacgtaattg catatccaac
    11041 tcctttatct tctgcgttaa gaacgcatgg atacccgttg tgagtgtttg tgttgttatc
    11101 gtctgcaact ttattgtgca gtgttgtgcc tgttaggtaa catttagttt ggctaaatgt
    11161 aaagatattg ctgttttatt gtttgttttt gcgagatgcg ccgcaccatt ccgaagcaaa
    11221 attcttaaac cgcacctttt tggtgctacc gctgatttgt ggtggtgcaa cctggttgcg
    11281 tcgcggctga tttcatcgct tacacagtaa ataaagcaac ttacatgcca aaaaatactt
    11341 aggggcgaat taattacgat tgtaatttta ggctgaaaaa gtcatatttc ggacagaatt
    11401 atcttttcgg catgggacgt ttgccgggat gataactgca cacgggaaaa atgcttttta
    11461 cctgtctttt caaggaagaa acaccttatg tctgcatctt cggttaagcc gttaaatgtt
    11521 caattacccg caataaccct tatccttttt gcactctgta ttgggatatt ttgttatctc
    11581 gcacaatgga tgagttatga agaggtcgat caatccgcac ttatccatct cggcgcaaac
    11641 gttgcgcctc ttactttgtc gggtgaaccc tggcgcttat tgagcagtat ctttctgcac
    11701 agtagtgttt ctcatctgct gatgaatatg tttgcattcc tggtcgtggg aggcgtggcg
    11761 gaacagattc tggggaaatg gcgactcctg attacctggt tattctccgg cgtctttggt
    11821 gggctcatca gcgcctgtta tgcgttacgc gaaagtgagc agatagtcat cagcgttggg
    11881 gcatccgggg cgattctggg aatagctggt gcagcgatag caacacagtt tgcttcaggt
    11941 acgggcacat accataaaaa ccagcggcga gtatttcctc tgttgggtat ggtggcgctg
    12001 acactgttgt acggtgcccg gcaaacagga atagataacg cttgccacat tggcggcctg
    12061 attgcgggtg gcgcgttggg ttggctgagc gcgcgtttat ctgggcaaaa ccgactcgtt
    12121 acggaaggcg ggattattgt tgcgggcagt cttcttctga ccggggctat ctggcttgcg
    12181 cagcagcaga tggatgagtc agttttacag gtcaggcaaa gcctgcgtga agcgttttat
    12241 ccacaggaga ttgaacaaga gcgacggcaa aaaaagcagc agttagcgga ggaacgcaac
    12301 gccctcaggg aaacattatc cgctccggta agtcgtgaac aggccagtgg tgatttgctc
    12361 gctgagattg ccgatatcca tgatatggcg atcagtcggg atggtaatat gttgtatgcc
    12421 gcaattgaaa acacgaacag cattgttgtt ttcgacctcg gacaaaagaa aatcctgcat
    12481 acctttacag cgcccatagc gaaagaaaag tcagtcaaac attgtggtgg ctgtaaagat
    12541 cagggcgtca gatcgctggc attaagcccg gatgaaaagt tgatttatgc gacttcattt
    12601 gaagcgaatg cgttatcggt cattaacgtg gcgacagggg agattattca gtcgattacc
    12661 accggtgcac atcctgacag ccttatcctc tcgcgtgatg gcacaaaagc ctgggtgatg
    12721 aatcgcacca gtaatagtgt gccagcgatt gatctggtga cttatcagca tgtggcggat
    12781 atcccgctgg agaaatacga cgggacgggg acgagcggta aaccaggcgc ctgggttatg
    12841 gcactttccc cggatgaaag aacattactg gttccgggag caggcagagg taacatcgtg
    12901 cggatcaata ccatcacgca tcagaaagaa gactttcccg caggtgatgc gcgtggaacg
    12961 atatcggcga tgcgttttcg acctgaaaac ggcgaggtta tttttgccga tagtcagggg
    13021 atttcacgta taagcgtagg ggatcaacaa gccagcatta tgacgcaatg gtgtagcagg
    13081 agcgtttatt ccgttgaggg tattagcccg gacggtcaat atttagcgtt ggtgtcatat
    13141 ggcctgcaag gttatgtcat cctgctcaat attaatgccg ggcagattat tggcgtttat
    13201 cctgccagct acgttaatca ccttcgtttt tcagcggatg gtagaaaaat atttgtcatg
    13261 gcgaagaacg ggttgatcca aatggacagg acgcgctcgc ttgatccgca ggcaattatt
    13321 cgtcatcccc aatatggcaa tgtggcttgt atccctgaac cgtaagaaaa aggggcatga
    13381 taaccatgcc ccaaagctca atgagaaagt acgttttgct catgcaggat acatcaggca
    13441 catcaacgtt tacgtgctcg cagactcatc aacacagcgg caaaaccaaa cagcgccgtc
    13501 agcacccaca gtggccagtt gccggtacgt gcatacggtg tgagtccggt ggtcggtgtc
    13561 acattagtgg tcaacacctc gcgggtgaac tgcgggatca tcgcctgaac ctccccctgc
    13621 gggccaatca ccgccgtaat gccgttgttg gtgctgcgca acagtgggcg cgccagctcc
    13681 agcgcacgca ttcgcgccat ctggaagtgc tgccatggac caatagactt accaaaccac
    13741 gcatcgttgg agatagtcag cagatagtcg gtatccgggc ggaagttatc gcgcacttgc
    13801 tcgccgagaa tgatctcgta gcaaatagcc gcagtaagct caataccatt taccgacagc
    13861 ggcggctgga tatatggccc acggctgaac gacgacatcg gcagatcaaa gaacggtgct
    13921 aacggacgca gaatcgactc cagcgggaca aactcgccaa acggcactag atggtttttg
    13981 ttatagcgat cggctgattc gtagctgtat ggtgcacctt tacccagtgt gatgatggtg
    14041 ttgtaggtat cgtagcggtt ctgcttattg agacgcgcgt cgacaatccc ggttaccagc
    14101 gagctacctt tatcgcgcaa ctcaccgtcc agtgctttga ggaacggttg ctgattaatt
    14161 tccagatcgg ttatcgccga ctccggccag ataatcaacg atgatttgcc catcagcgga
    14221 gccgttgcgt tgtagtaaat cttcagcgta ttaagaagct gatctccatc ccatttcagt
    14281 gattgcggaa tatcgccctg aaccatcgaa acctgaatgg ttttctccgg ttgcggggta
    14341 aaccattgga tgtaacgcag cgggaagggt agggcaaaca gcacgacggc caccaccagc
    14401 ggacgccagt tgcgtttgac caacgccagt gccagcaggc cactaaccat catcagcagg
    14461 aagttaatgg cttccacgcc cattatcggt gccagccctt ttaacgggcc atcaatctgg
    14521 ctatagccga actgtaacca cgggaagcct gtcagtaccc aaccgcgcag aaactcggtc
    14581 acttgccaga gagcaggggc ggcaatcgcc acgcgcagcc aggtggtttt cggccacaga
    14641 cgcgacagca cgccagcaaa cagtccggta tacagcgaca aatacgccgc cagcagcacc
    14701 accaggaaga tgttaaccgg gccaggcatt ccgccaaagg tcgcgatgct gacatagacc
    14761 cagttaatac cgctgccaaa gaggccaaat ccccagcaaa agccaatagc ggcagactgg
    14821 agtggacggc ggttaaaggt caacgcctga agccccatca acgaaataat cgccgcaggc
    14881 cagacgtcgt aaggagagaa ggccagcgtt ccgcaggcac cgaataataa cgccagcagc
    14941 aggcgaatgc gctggcgttc aattaatgag gcaaaagcca tgtagttatc tatccagttt
    15001 cggttttatt catccagctt cggctggggt gagtcatccg ggattttgac atgaacctga
    15061 ataatacgcc gactgtcggc catcgccact ttgaactggt aaccgtcgat gtcgatagtt
    15121 tcgccacgcg ccggaagatg cccaaatgcc tgcatcacca gaccaccgat agtgtcaacc
    15181 tcttcatcgc taaagtgggt gccgaacgct tcgttgaagt cttcaatgga agccagtgca
    15241 cgcacggtcc aggtatgacg actcagctga cggaagtcga tatcatcttc ttcgtcatac
    15301 tcgtcttcaa tctcaccaac aatcagttcc aggatgtctt caatggtcac cagaccggaa
    15361 accccaccga attcgtcaat gacgatcgcc atgtggtaac gctgagagcg aaactctttc
    15421 agcatccggt ctacgcgctt actttcagga acgacaaccg cctgacgtaa cactttgtcc
    15481 atgctgaagg cttcagcatc gctgcgcata aacggcagca agtctttcgc catcagaatc
    15541 ccttcaatgt gatctttgtc ttcgctaatc accgggaaac gtgagtgggc ggactcgatg
    15601 atgacatcaa ggcattcgtc cagcgtctgg ttgcgtttca gggtaatcat ctgggagcgg
    15661 gggatcatga tgtcgcgaac gcgttggtct gcgatgtcca tcaccccttc gagcatatcg
    15721 cgcgtatctt cgtcgataag gtcgttctgc ccggaatcac ggatcagcgc cagcagttcg
    15781 tcacggtttt tcggttcacc gtggaaaagt tggctgagta acagggagaa aaatcccttc
    15841 ttgttgctta tcgtgtcact actgtgtgaa ttgtcgtcgc tcatggcgtc gtatgggttc
    15901 tcatgttagt taatcaaaac gtcgtcgtta atcaccaacg gcggggacgt ctgccagtca
    15961 aatgcctggc aaattattct ttctcggcaa tgtacggatc ctcatagccc agagcaagca
    16021 taatctctgt ttcgagggct tccatttctt ctgcttcgtc atcttcgatg tgatcgtaac
    16081 ctaacaaatg cagactgccg tgcaccacca tatgcgccca gtgcgcctcc agtggtttgc
    16141 cttgctcctg agcttccttc tcaaccacct gacggcagat aaccagatcg cccagtagcg
    16201 acatttccat gccaggcggc acttcaaacg ggaaggagag cacgttggtc ggcttatcct
    16261 taccgcgata ggtcagattc agactgtggc tttcggcggt atcgaccacg cgaatagtca
    16321 cttccgattc ttcctgaaac tgcgggatca ccgcattcag ccatgtttga aactggctct
    16381 cttccggtaa cccggaatta tcttcacatg ccagttgtaa atcgaggatc acctgactca
    16441 tttttgttcc tgttcttcgc gcttgcgttc tgccgccagc gccgcttttc gtttttgttc
    16501 ggcttcttcc caggcttcat aggcgttaac gatacgcgcc accacagggt gacgaaccac
    16561 gtcttcgctg tggaagaagt taaagctgat ctcttcgaca tcggccagca cttcgatggc
    16621 gtgacgtaag cctgatttag tattacgcgg caggtcgatc tgtgtgacgt cgccggtgat
    16681 aaccgctttt gagttaaaac cgatacgggt caggaacatc ttcatctgtt cgatggtggt
    16741 gttctggctc tcatcgagaa tgataaacgc gtcgttcagc gtacgaccac gcatataggc
    16801 cagcggcgcg acttcaataa cgttgcgctc aatcagtttc tcaactttct caaagcccag
    16861 catttcaaac agcgcgtcgt acagtgggcg cagatacggg tctactttct ggcttaaatc
    16921 gccaggcagg aagcccagtt tctcaccggc ttcgaccgcc ggacgagtca gcagaatacg
    16981 gcgaatctcc tgacgctcca gggcatcaac tgccgcagcc actgccaggt aggttttacc
    17041 cgtacccgcc gggccaacgc cgaaggtaat gtcatggtcg agaatattgg cgatgtactg
    17101 cgcctggttt ggcgtgcgcg gcttaattac gccgcgtttg gttttgatat tgaccgcttt
    17161 gccgtactcc ggcacgctct ccgcactttg ctccagtact cgcgcttctt taatcgcaag
    17221 gtggatctgt tccggttcga tatcctgaat ctgaccgcgc atcggggctg tatcgacata
    17281 caggctacgc agaatgtctg ccgcagcggt gacgcaaatc ggacggccgg tcagtttaaa
    17341 gtggttgtcg cggcgattga tctcgatgcc gagacggcgt tcgagctgct tgatgttgtc
    17401 atcaaacggg ccgcacaggc tcaacagacg cgcgttgtct gctggctcca gggtgatttc
    17461 gcgagtgtct atgttcaaac cgttcctctt atctgtatgc cgccggaagc tgaacattca
    17521 ccggcctata aggaaattat tcacgccaca ggaaaaaggc gcaagcattg cgataaagat
    17581 ggggataaag agagaaaaaa caaggcccac cggaacggcg ggcctgagaa ttacggctga
    17641 taataaccca cgccaaggtc gttttctttg cgggtacggg caatcaccga ttccggtgtt
    17701 tctgccacgc gcagacccat ttcatcttca gtacgcacca ctttaccgcg cagagagttc
    17761 gggtagacgt cggtaatttc tacatcgacg aatttaccga tcatatccgg tgtgccctcg
    17821 aagttgacca cgcggttatt ttcggtacgc ccggaaagct ccatgatgct cttacgcgaa
    17881 gtcccttcta ccagaatacg ctgggtggtg ccgagcatcc ggcggctcca tgccatcgct
    17941 tgctgattga tacgttcttg cagaatatac agacgttgct tcttctcttc ttccggaaca
    18001 tcatcaacca tatcggcggc tggtgtaccc ggacgtgcag agaagataaa gctgtagctc
    18061 atgtcgaaat tgacgtcggc aatcagcttc atcgttttct cgaagtcttc ggtggtttcg
    18121 ccagggaagc caacaatgaa gtcagaactg atctgaatat ccggacgcgc cgcacgcagt
    18181 ttacggatga tcgctttgta ctccagcgcc gtgtgagtac ggcccatcag gttcagaatg
    18241 cgatcggaac cgctttgtac tggcagatgc aggaagctca ccagctccgg cgtgtcgcga
    18301 tacacttcga tgatatcgtc ggtgaattcg atcggatggc tggtggtaaa gcgaatacga
    18361 tcgatcccgt cgatcgcagc aaccagacgc agcagatcgg caaacgatcc ggtggagccg
    18421 tcgtagtttt caccacgcca ggcgttcacg ttctgaccga gcaggttgac ttcacgcacg
    18481 ccctgagccg caagctgggc aatctcaaac agaatatcgt cagacggacg gcttacctct
    18541 tcaccacggg tgtaaggcac cacgcagtag gtgcaatatt tattgcagcc ttccatgatg
    18601 gagacaaacg cggtcggccc ttcggcgcgc ggttccggta gacggtcaaa cttctcgatt
    18661 tccgggaagc tgatatctac aaccgggctg cggtcgccgc gcacggagtt gatcatctcc
    18721 ggcagacggt gcagcgtttg cggcccaaaa ataatatcga catagtgggc gcgctggcga
    18781 atgtgctcgc cttcttgcga tgccacgcag ccaccgacgc cgataatcag gtctggattc
    18841 ttctctttta acagtttcca gcgacccaac tgatggaaga ctttttcctg agccttctcg
    18901 cggattgagc aggtgttcag cagcagcaca tccgcttctt ccgccacgtc ggtcagttga
    18961 tagccgtggg tggcatccag cagatcggcc atcttcgatg aatcgtactc gttcatctga
    19021 cagccccagg ttttaatatg gagttttttg gtcatcgact tgctcttgcg aaatagtagc
    19081 caggaatgca gggcgtatag tgtaatgctt tgctgccgtt gtgaccagta tgagcgttat
    19141 cagcccttgg gggtaaaaat cctgtaaact taaagcatat tgctaacagg atgattgacc
    19201 atgacaaatc aaccaacgga aattgccatt gtcggcggag gaatggtcgg cggcgcgctg
    19261 gcgctggggc tggcacagca cggattttcg gtaacggtga tcgagcacgc agaacctgca
    19321 ccgtttgtcg ctgatagcca accggacgtg cggatctcgg cgatcagcgc cgcatcggta
    19381 tcattgctta aagggttagg ggtctgggat gcagtacagg ctatgcgttg ccatccttac
    19441 cgcagactgg aaacgtggga gtgggaaacg gcgcatgtgg tgtttgacgc cgctgaactt
    19501 aagctaccgt tgcttggcta tatggtggaa aacaccgtcc tgcaacaggc gctgtggcag
    19561 gcgctggaag cgcatccgaa agtaacgtta cgtgtgccag gctcgctgat tgcattacat
    19621 cgagataatg atcttcagga gctggaactg aagggcggtg aaacgatccg cgcgaaactg
    19681 gtgattggtg ccgacggcgc aaattctcag gtgcggcaga tggcgggaat tggcgttcat
    19741 gcctggcagt atgcgcagtc gtgcatgttg attagcgtac agtgtgagaa cgatcccggc
    19801 gacagcacct ggcagcaatt taccccggac ggaccgcgtg cgtttctgcc gttgtttgat
    19861 aactgggcat cgctggtgtg gtatgactct ccggcgcgta ttcgccagtt gcagaatatg
    19921 aatatggcgc aattccaggc ggaaatcgcg aagcatttcc cgtcgcgtct gggttacgtt
    19981 acaccgcttg ccgctggtgc gtttccgctg acgcgccgac atgcgttgca gtatgtgcag
    20041 ccggggcttg cgctggtggg cgatgccgcg cataccatcc atccgctggc ggggcagggg
    20101 gtgaatcttg gttatcgtga tgtcgatgcc ctgattgatg ttctggtgaa tgcccgcagc
    20161 tacggtgaag cgtgggccag ttatcctgtc ctcaagcgtt accagatgcg gcgcatggcg
    20221 gataacttca ttatgcaaag tggtatggat ctgttttatg ccggattcag taataatctg
    20281 ccgccgctgc gttttgtgcg taatctcggg ttaatggcgg cagagcgtgc tggcgtgttg
    20341 aaacgtcagg cgctgaaata tgcgttaggg ttgtagcctt acaacattgc cggatgcgtg
    20401 ccaaccgtag gtcggataag acgcggcagc gtcgcatccg acattgaagg ataagacgtg
    20461 tcaacatcgc attcgacatt gaatgaacgc agaaaagcaa aaagctcgcc gaagcgagct
    20521 tttttaatgt ggctggggta cgaggattcg aacctcggaa tgccggaatc agaatccggt
    20581 gccttaccgc ttggcgatac cccaactggt gcacttacga tgtaagcgtc ttgaataaat
    20641 tggctggggt acgaggattc gaacctcgga atgccggaat cagaatccgg tgccttaccg
    20701 cttggcgata ccccaacaaa ttggtttttg aatttgccga acgtattcga cacattcaga
    20761 atttggtggc tacgacggga ttcgaacctg tgaccccatc attatgagtg atgtgctcta
    20821 accaactgag ctacgtagcc agattgtttc ttcgatggct ggggtacctg gattcgaacc
    20881 agggaatgcc ggtatcaaaa accggtgcct taccgcttgg cgatacccca ataaccggtc
    20941 ggtgaaccgc ttactcgaag aagatggctg gggtacctgg attcgaacca gggaatgccg
    21001 gtatcaaaaa ccggtgcctt accgcttggc gataccccat ccgtacaacg ctttctggtg
    21061 aatggtgcgg gaggcgagac ttgaactcgc acaccttgcg gcgccagaac ctaaatctgg
    21121 tgcgtctacc aatttcgcca ctcccgcaaa aaagatggtg gctacgacgg gattcgaacc
    21181 tgtgacccca tcattatgag tgatgtgctc taaccaactg agctacgtag ccatcttttt
    21241 ttcgcgatac cttatcggcg ttgcggggcg cattatgcgt atagagcctt gcagcgtcaa
    21301 cctctttttc aaggaaaatt gctcgaaagt gactgtttgg ttaggttgcg aacagcgtgg
    21361 cgctatattc gtcaattatt gtttactttg tgttttttcc ccaccctaca gccattcttt
    21421 tgtcatacag gatgaaattc ggaattcaac aatggtggtg ggaaattaat ctatgaaata
    21481 ctggcctaca gtgatgagtt gtcaaacagt gatgtggcaa acccggaaca tttccttact
    21541 gcatatcaga atcaacaagc acctcaataa ctgaaacagc cccggatttc accggggctg
    21601 tttcgcattt cttacttata cgccgactgg tgaacgccaa ccgcacgacc agacggatcg
    21661 tccattttct tgaacgcctc atcccactca atcgctttag cggaagaaca cgcgacggac
    21721 gggccgcccg gcacgcactc agcggcgctc ggaagcggga acagttcttc aaagatctcc
    21781 cggtacagat acgcttcttt tgaggttggc gtgttgtacg ggaagcggaa gcgggcagtt
    21841 tccagttgct gatcagaaac ctgctgcgcc gccacttctt tcagggtgtc gatccaactg
    21901 taaccgacgc catcggagaa ctgctctttc tgccgccagg ccacgcttgc gggcaggtat
    21961 gactcaaaac attcacgcag gatgtgtttt tccattttgc cgttaccgca cattttatcc
    22021 tgcgggttaa tgcgcatcgc cacatcaagg aattttttgt cgaggaacgg aacgcgtgct
    22081 tccacgcccc aggctgacat cgctttgttg gcgcgcgcgc agtcatacat atgcagggcc
    22141 agcagtttac gcaccgtctc ttcatgcagt tctttagcgt tgggcgcttt atggaagtaa
    22201 agataaccgc caaacacttc atcagaacct tcaccggaca gcaccatttt aatgcccatc
    22261 gccttgatct tacgcgacat taaatacatc ggtgttgaag cgcgaatagt ggtcacatca
    22321 taagtttcga tgtggtaaat cacgtcgcgg atggcatcca gaccttcctg tacagtgaag
    22381 tgaatttcgt gatgcaccgt gcccagatgg tttgccactt cctgggctgc tttaagatcc
    22441 ggtgaacccg gcagacctac agcaaaggag tgtaactggg gccaccaggc ttcagagcgt
    22501 tcctgatctt ccacgcgacg ggctgcgtat ttcttggtga tagcggaaat aattgaggaa
    22561 tccagaccac cagaaagcag cacaccgtaa ggcacatcag acatcagatg gcttttaacg
    22621 gaatcttcca gagcctgacg cagctcgttt ttgtcggtca cgttatcttt cactgcatca
    22681 aagtcgaacc agtcgcgatg ataataagaa cggatttcgc cgtcctggct ccacaaatag
    22741 ctccccgccg ggaactcttt aatcgtgcgg caaactggca ccagcgattt catttctgag
    22801 gccacataca gctgaccgtg ttcgtcatac cccatataca gtgggatgat ccccagatgg
    22861 tcgcgaccaa tcaggtaggc atctttttcg ctgtcgtaca gggcaaaggc aaacatgccc
    22921 tgcaagtcgt cgagaaattc cggccctttt tcctgataca gcgcgaggat cacttcacag
    22981 tcagaccctg tctggaactg ataacgatcg ccatattcag cgcgcaatgc ctggtggttg
    23041 tagatttcac cgtttaccgc cagtacgtgg gttttttgtt ggttgtagag aggttgcgcc
    23101 cccgcgttaa cgtcaacaat tgacagacgt tcgtgagcga gaatggcgtt atcgctggca
    23161 taaataccgg accagtccgg gccacgatga cgcatcaggc gtgacagctc gagtgctttc
    23221 ttacgctgct caactgcgtc tgttttgata tcgaatacgc caaaaattga acacataacc
    23281 ttctccgtta acctggtatt tgttgcttgt tgtgtttgct tgtttaaaaa aatgccgcaa
    23341 agcagcactg tgcgcaagcg atttggcggt gaaaaaataa aaaacgtaat ggtgattgtc
    23401 gattggtgaa aaagggtctg gtgtgaggat gtatttattg atgaatcgat aatttttagc
    23461 gggttttatt gaatggttat attttacggg g
//
LOCUS       DAZPAC010000051        22511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZPAC010000051 DAZPAC010000000
VERSION     DAZPAC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.051"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..469
                     /locus_tag="ACHUPM_001666"
     CDS             <1..469
                     /locus_tag="ACHUPM_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0869470.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            582..1376
                     /gene="panB"
                     /locus_tag="ACHUPM_001667"
     CDS             582..1376
                     /gene="panB"
                     /locus_tag="ACHUPM_001667"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR0869471.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1388..2239
                     /gene="panC"
                     /locus_tag="ACHUPM_001668"
     CDS             1388..2239
                     /gene="panC"
                     /locus_tag="ACHUPM_001668"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR0869472.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2531..3427)
                     /gene="rpnC"
                     /locus_tag="ACHUPM_001669"
     CDS             complement(2531..3427)
                     /gene="rpnC"
                     /locus_tag="ACHUPM_001669"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR0869473.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3701..4081
                     /gene="panD"
                     /locus_tag="ACHUPM_001670"
     CDS             3701..4081
                     /gene="panD"
                     /locus_tag="ACHUPM_001670"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR0869474.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4085..5314)
                     /locus_tag="ACHUPM_001671"
     CDS             complement(4085..5314)
                     /locus_tag="ACHUPM_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR0869475.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5378..5818)
                     /locus_tag="ACHUPM_001672"
     CDS             complement(5378..5818)
                     /locus_tag="ACHUPM_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR0869476.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5923..6693)
                     /locus_tag="ACHUPM_001673"
     CDS             complement(5923..6693)
                     /locus_tag="ACHUPM_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0869477.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6690..7616)
                     /locus_tag="ACHUPM_001674"
     CDS             complement(6690..7616)
                     /locus_tag="ACHUPM_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0869478.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7725..8387
                     /gene="can"
                     /locus_tag="ACHUPM_001675"
     CDS             7725..8387
                     /gene="can"
                     /locus_tag="ACHUPM_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR0869479.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8428..8964)
                     /gene="hpt"
                     /locus_tag="ACHUPM_001676"
     CDS             complement(8428..8964)
                     /gene="hpt"
                     /locus_tag="ACHUPM_001676"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR0869480.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9170..11560
                     /gene="gcd"
                     /locus_tag="ACHUPM_001677"
     CDS             9170..11560
                     /gene="gcd"
                     /locus_tag="ACHUPM_001677"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR0869481.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11607..13157)
                     /gene="cueO"
                     /locus_tag="ACHUPM_001678"
     CDS             complement(11607..13157)
                     /gene="cueO"
                     /locus_tag="ACHUPM_001678"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR0869482.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13323..13670
                     /locus_tag="ACHUPM_001679"
     CDS             13323..13670
                     /locus_tag="ACHUPM_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR0869483.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13776..14642
                     /gene="speE"
                     /locus_tag="ACHUPM_001680"
     CDS             13776..14642
                     /gene="speE"
                     /locus_tag="ACHUPM_001680"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR0869484.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14658..15452
                     /gene="speD"
                     /locus_tag="ACHUPM_001681"
     CDS             14658..15452
                     /gene="speD"
                     /locus_tag="ACHUPM_001681"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR0869485.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15490..15852)
                     /gene="yacL"
                     /locus_tag="ACHUPM_001682"
     CDS             complement(15490..15852)
                     /gene="yacL"
                     /locus_tag="ACHUPM_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR0869486.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16027..18624)
                     /gene="acnB"
                     /locus_tag="ACHUPM_001683"
     CDS             complement(16027..18624)
                     /gene="acnB"
                     /locus_tag="ACHUPM_001683"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR0869487.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKIEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18979..20736
                     /locus_tag="ACHUPM_001684"
     CDS             18979..20736
                     /locus_tag="ACHUPM_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFR0869488.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20807..22231)
                     /gene="lpdA"
                     /locus_tag="ACHUPM_001685"
     CDS             complement(20807..22231)
                     /gene="lpdA"
                     /locus_tag="ACHUPM_001685"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR0869489.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
BASE COUNT         5566 a         6105 c         5482 g         5358 t
ORIGIN      
        1 tagtggaact tctgtgactg gttctacggt aaaaatgggt gaatatagtg cagaacaaat
       61 cagaaacggt gccacaccgg ttccttttga tatttcactt caaaactgcg ttcgtgtgac
      121 taatattgag acaaaattag tttcaacaaa ggttggtact gaaaacgggc aactccttgg
      181 taatactctc acaggtaatg acgcagcaaa aggagtcggt gtactcatag aaggtttagc
      241 aactagtaaa aatcctctaa tgacattgaa acctaatgat tcaaattctg tttataaaga
      301 ttacgacccg agaggcaaag acgatacaac aggaggagtt tacccggatc aagatacagg
      361 tataacatac cctctccatt tccaggccac gctacaacag gatggaacta taccaataga
      421 agctggtgag tttaaagcca ccagtacttt ccaggtaacc tacccttaat aagtccaccg
      481 caccgccatc atctggcggt gcggtaattg ataaatcatt gcccccacca atgtgactga
      541 taaaatgcca ggcaacacga catcatttat caggacacgt tatgaaaccg accaccattg
      601 cttcacttca gaaatgtaag caggataaaa agcgtttcgc gaccatcacc gcttacgact
      661 acagcttcgc gaaactattt gctgatgaag gacttaacgt catgctggta ggcgactcgc
      721 tgggcatgac ggttcagggt cacgactcca ccctgccggt gaccgttgcc gatatcgcct
      781 accacactgc ctccgtacgt cgtggtgcgc caaactgcct gctgttggct gacctgccgt
      841 ttatggcgta tgccacgccg gaacaagcct ttgaaaacgc cgcaacggtt atgcgtgccg
      901 gtgccaatat ggtcaaaatt gagggcggtg agtggctggt cgaaaccgta aaaatgctga
      961 ccgaacgtgc cgttcctgta tgtggtcact taggtttaac cccacagtca gtgaatattt
     1021 tcggtggcta caaagttcag gggcgcggcg atgaagcggg cgatcaactg ctcagcgatg
     1081 cattagcctt agaagccgct ggggcacagc tgctggtgct ggaatgcgtg ccgattgaac
     1141 tggcaaaacg tattaccgaa gcactggcga tcccggttat tggcattggc gcaggcaacg
     1201 tcactgacgg gcagatcctc gtgatgcacg acgccttcgg cattaccggc ggtcacattc
     1261 ctaaattcgc taaaaatttc ctcgccgaaa cgggcgacat ccgcgcggct gtgcggcagt
     1321 atatggctga agtggagtcc ggcgtttatc cgggcgaaga acacagtttc cattaaggag
     1381 tcacgttgtg ttaattatcg aaaccctgcc gctgctgcgt cagcaaattc gccgcctgcg
     1441 tatggaaggc aagcgcgtgg cgctggtgcc tactatgggt aatctgcacg atggccatat
     1501 gaagctggtc gacgaagcca aagcccgcgc cgatgtggtc gtcgtcagta ttttcgttaa
     1561 cccgatgcag ttcgaccgcc cggaagatct ggcacgttat ccacggacct tgcaggagga
     1621 ctgcgagaag ctaaacaaac gtaaagtgga tttagttttc gccccttcgg taaaagagat
     1681 ctacccgaac ggtactgaaa cccatactta cgttgacgtt cccgggcttt cgaccatgct
     1741 ggaaggcgcg agccgtccgg ggcattttcg cggcgtttcg actatcgtca gcaagctgtt
     1801 taacctggtc cagccggaca tcgcctgctt cggtgaaaag gactttcagc aactggcgct
     1861 aatccgcaaa atggttgccg atatgggctt cgatattgaa attgtcggcg tcccgattat
     1921 gcgcgccaaa gatggccttg cgctcagttc ccgtaatggt tatctgacgg cggaacaacg
     1981 caaaattgcg cccggtctgt acaaagtttt aagttcaatt gccgacaaat tgcaggctgg
     2041 cgagcgggat cttgatgaaa ttattgccat tgcagggcaa gaactgaatg aaaaaggctt
     2101 ccgcgccgat gatattcaga ttcgcgatgc cgacacgttg ctggaagtct ctgaaaccag
     2161 caaacgagct gtaattctgg tagccgcctg gcttggcgat gcccgcctga tcgacaacaa
     2221 aatcgtcgaa ctggcgtaat gcttttactc taaataattc aagttgcagg aaggcggcaa
     2281 gcgagtgaca aattcgtcag gaacgaattt gcccagccgc aggctggcct tcggtgagag
     2341 acatggatgt ctctcattaa tccccaggag cttacataag taagtgactg gggttagcga
     2401 acgcagacgc agcacatgca atttaaagta tgacgagtat aactggcgct acggctgatg
     2461 gcgccaatta ttaatttgcc ccacgtcatg gcgatggcgt ctgtaagcaa ttattcataa
     2521 cgcagacaac ttagttaatc agcctatcaa tctcagcaag agataaattt gtcatctctg
     2581 caacgtcttc ccgagacatc cctttactca gaaaacgctg ggcgaattct tgcctttcct
     2641 cctgtcttcc ctgctgaatc cccttctcaa tccctttctc ttcaaaccac tgcgccagcg
     2701 tcatcataga ctcccctccc gtttccctgt ctctcagcac accataaaac aaatccgctt
     2761 gttcagtatg accgcgttgc agcatatagt tttgcatggc aactaactga cttccgctag
     2821 tgtacccttc gtcaatcagc gtgaccagtt gctccagcaa taacattaag tcgcgctggc
     2881 gaatatgttt ttgcagtagt tcgagaatcg caatccgccg atgttgcatg atttcgtcat
     2941 ccggtgtgat ggtgatatcc accagcggga aaggactgtt atagacgcgt cgtgccagct
     3001 ccggcgagta aaatatatca aaccagcaca ttgagagcgg ataaggtgtg gcctcgccct
     3061 gataaaacag tatcggcacc accagcggca gcttatcgtg atccgcctcc agatgccggt
     3121 gcatggcagc aatagaataa cgcatcatgc gaaagaccat tttcttatcc ggcttgcttt
     3181 ggtgttcaat cacaacatgc agataaccgg gattgccctg catttgcacg gaatagagca
     3241 cgtccgtgct gtgtcctttc aggctctctt caatgaaact ccccgactct aaatgaagcg
     3301 tgttgaggtc acaaagttct cgtaattcca ctggcaaatg tatctccaga aagtcgcgag
     3361 ccgtctccgc gtgcattaaa aattgtttaa acactgcatc atgcggtgtg gtactcggtg
     3421 catccatctt ccctgccttc cgtgttgtct tgcgtgaagg cagtatccag tcaaggcgtg
     3481 ccatcgtcag ccacctcttt agtgtttacc gagcagcgtt cagagaaatt tcaattggtt
     3541 acagccgtta catggcacgc ttcggcacgg tacgcaaact gctgatgtaa atgccgtcag
     3601 atgagtagac actaaacaaa aatcgggcaa tactgcgtga gaatttcctc aaagcaggca
     3661 tcgcctgctt cgttaacgac agggtagaaa ggtagaagtt atgattcgca cgatgctgca
     3721 gggcaaactc caccgcgtga aagtgactca tgcggacctg cactatgaag gttcttgcgc
     3781 cattgaccag gattttcttg acgcagccgg tattctcgaa aacgaagcca ttgatatctg
     3841 gaatgtcacc aacggcaagc gtttctccac ttatgccatc gcggcagaac gcggttcgag
     3901 aattatttct gttaacggtg cggcggccca ctgcgccagt gtcggcgata ttgtcatcat
     3961 cgccagcttc gttaccatgc cagatgaaga agctcgcacc tggcgaccca acgtcgccta
     4021 ttttgaaggc gacaatgaaa tgaaacgtac cgcgaaagcg attccggtac aggttgcttg
     4081 attgttatcc ctgcggctgg ttactcacca gccgcgacat cgtctccagc gaatccgttc
     4141 ttaagatata aagtcgtttt agtaacaacg gattatcccc cggttttact ttgcctttca
     4201 tggttgtcac cgccaggtga aatccggcat cgtttgctgc cttcacggct ttgtcattaa
     4261 atccgccaaa cggatacgaa agataccaga catgcggatt aaattgcgcc agagcgcggc
     4321 gtgaacgtgc aaaatcaaac agaatattgt gctcactacg gctcagtaat atgggtcggc
     4381 gatagccatc cacccgatgc aaaaaatggg tatgtgactg aaaatcaaaa acatcgcgaa
     4441 tttcgttaag ctcagaaacg ctcataaatt gcagcgattt tgggttccac ttctgcgggt
     4501 gacgtttgat gcgcgaggta acaacaaacg ccgtcgcctt catgccatat tgtttcaata
     4561 caggatacgc atagcggctc accgacttga ggccatcatc aaaggtaatc accaccgctc
     4621 gcgcagggag attgatctta ttcttcacgt aaccttccag ctgcgccatg ctcagtgtcg
     4681 cgtatcccct gtcgcgcagc caggccattt ggttattgaa agcgcgtacc gaggtggtcg
     4741 tcgaagtatg gcgaaaacgg gtgttttctt cgtcgcgcag aatatggtga taagttagca
     4801 ccggaaggcc attatcgggt tgggcatcca gcgcgctgat ataggccagt cgatcgccaa
     4861 tacggacctg ataccaggtt tgatttaacc tgtctttcag tttatgcaaa atcgggtagc
     4921 gcaaattgtc cgccaatacc ccaaatggcg cactgcccac actcggcgcg ttatagaccg
     4981 gcgtatcttt ccaggtaact aagttctgat tactcagcgg cttgttgagg tcacccaaac
     5041 cgtcttcaac tttttgtcgc ccctgaaccg gctcgagatg ccctttatcg ataaaccctt
     5101 taccaaagcc aaaattaaat gcgtaataac ttgcggcagt gggctccacg gcaatgattt
     5161 gtccggcccg aatattcccc acggtcacca tcttgtcgcc aatttgcgcc cagaccgcag
     5221 cattttcgat ggtttgcata taacgggcag gtaacgcggc actgacactt gcggtaaaca
     5281 gcatcagcag gagaataaca gcttgtttgt acatgaaaaa ataaccgagg gtgaaacaac
     5341 gcttttattg tagcaaaagc gctataagaa tcgtgggtta atactcatac aaattatgtt
     5401 tgaggacgaa aggcggattt ttttgttgtt ggtgccaaag actactcacc tccggcccac
     5461 ccagttcgac aatacgctcg cgaaactctg aactggtcat ggtttcacgg caagccatca
     5521 tctgttcaat taacggtaac gtgacaccag aaatcacttc gcaacgggag tgtttgtggc
     5581 ttaataatga agccacgcgg tacggtggcg ctccggctat atcggttaag aagatgacac
     5641 cctcaccaga gtccgcttca tgcagagcat cgcacatcat gcggctgagc atattagagc
     5701 ttaacccacg ccagaaattc acggcccggc actgaagaag cgccccatgt tttttctcca
     5761 gcgcatccag tatctcttgc gcccggtcat cgtgacaggt aattacccaa cctaacatcg
     5821 ccttcctcct tagccgaaaa tcagtctacc ctgaccattg ttgataaagg gtgatagccg
     5881 tcaaaactcc tctcccccaa atccagagga gaggaaatag ccttagctac gcaaaccacg
     5941 tccacgttgg atcagcgacc aacagatcaa ataaaacgcc acaataaaga ccaccagtac
     6001 gccaaaggta gtgaccagcg gaacatcatt gataccgagg aagccgtagc ggaaaccact
     6061 gatcatataa acgattgggt tcaggtgcga cagcccttgc cagaacggcg gcaacaaagt
     6121 cagtgagtaa aagaccccac ccaaatacgt gagtggcgtt aacacaaagg ttggcaccag
     6181 gctgatgtca tcgaacgttt tggcaaacac accgttcagc aaacccgcaa gggagaacaa
     6241 caccgccgtg agcaccaggg ttaaggcaac gaatacccac gaatgcacct gaaacggcac
     6301 aaaaaacagt gaaattgccg tcaccagaat gccaacaaac agaccacgcg ccacgccacc
     6361 gccgacatat ccggcaataa tgacgtgagt cggaaccggc gctaccagca gctcttcaat
     6421 attacgctgg aacttggcac caaaaaatga tgacgcaacg ttggcgtagg cattggtgat
     6481 caccgacatc atgatcagcc ccggtacgat gaactgcata tagctgaagc catgcatatc
     6541 gccaatacgc gaaccaatca ggttaccgaa gataataaag taaagggtca tggtgatgac
     6601 tggcggcacc agcgtctgca cccagatacg cataaagcga tggatctctt tcgcccagat
     6661 gctttttagc gccacccagt aaagatgcat catgcgcgat ctccttgttt ttcattaacc
     6721 agtgaaacaa acagctcttc cagacggtta gctttgttac gcatacttaa tacctgaatg
     6781 ccctgctcac ttaactgcgt aaatacgctg ttgatcccct gctcacgcag cacttcaact
     6841 tccagcgtcg cggtatcgac cagtcgatac tgatagccat cgagcttcgg taacggactt
     6901 ttcggtgcga gatcgagaat aaaggtttcc gatttcagct tcgccagcag cgccttcatc
     6961 gaggtatttt ccaccagctc accgtgttga ataatgccga tattgcggca cagcatttct
     7021 gcttcttcca ggtagtgtgt ggtgagaatg atggtggtgc ctttgtcgtt taaatccttc
     7081 aaaaagcccc acattgagcg gcgaagttca atatccacgc ctgcggtcgg ttcgtcgaga
     7141 atcagtagtt taggttcatg cattaacgca cgggcaatca ttaaacggcg cttcatcccg
     7201 ccagataaca tacgcgcacg ttcgttgcgt tttccccata gatcgagttg tttaagatat
     7261 ttttcgctgc ggatgtacgc ctctttgcgc tccacaccgt agtaccctgc ctgattcacc
     7321 acaatttgct gcacggtttc aaacgggttg aagttaaatt cctgcggcac cagtcccaac
     7381 tgacgtttag cgttcacgac atctttctcg agatcgtaac caaatacgct gacccgcccg
     7441 gaggttttat ttaccagaga gctgataata ccgatagtgg tcgatttccc ggccccgttc
     7501 ggcccgagaa gcgcataaaa atcacccgct tcgacctgca aatctattcc acgaagcgcc
     7561 tgaacgccgc ctggataggt ttttttaagc tgttgaagtt ccagtgcaat ggtcataaat
     7621 ttttacttac cttacgttct tacactttat ctgtggttta aatcgtcccg gagttgccct
     7681 atattagcca aacgtaatta tttggttaca ggtcgttaac ctccatgaaa gacatagata
     7741 cactcatcag caacaatgca ctatggtcaa aaatgctggt ggaagaggat cccgggtttt
     7801 ttgagaaact ggcacaagcg caaaaaccgc gctttctatg gattggatgt tccgacagtc
     7861 gcgttcctgc agaacgttta accggtcttg agccgggcga actctttgtt caccgtaatg
     7921 ttgctaacct ggtcattcac accgacctga actgcctttc cgtggttcag tatgcagtgg
     7981 atgtactcga agttgaacac attattatct gtggccacta cggttgcggc ggcgtacaag
     8041 ccgccgttga aaacccggaa ctggggctta tcaacaactg gctgctgcat atccgcgata
     8101 tctggttcaa acatagctca ttgctcggcg aaatgccgca agagcgccgt ctggatacct
     8161 tgtgtgaact gaacgtcatg gaacaggttt ataacctagg ccactccacc attatgcaat
     8221 cagcgtggaa acgcgggcag aaagttacca ttcacggctg ggcctacggc attcacgacg
     8281 gcttgctgcg tgatctggat gtcacagcga ccaaccgcga aacccttgag caacgttacc
     8341 gtcacgggat ttccaacctc aagctgaaac acgccaacca caaataaaaa tgccatgccg
     8401 gatgcaacac atccggcaac ttcacactta ctcgtcccgc agaatcactt tgccgatata
     8461 cggcagatga cggtaacgct gtgcgtaatc aatgccgtaa cccaccacaa actcatccgg
     8521 gatcgagaaa ccgataaatt ctaccgggac gttcacttca cgacgggacg gtttatccag
     8581 cagcgtacaa atcgccagcg acttcggttc gcgcaggctt aagatctcac gcactttcga
     8641 cagtgtattc cccgagtcga tgatatcttc aacaatcagc acgtccttgc cacggatatc
     8701 ttcatccaga tctttgagga ttttcacatc acgggtggtg gacatgccgc taccgtagct
     8761 ggaggcggtc ataaagtcga cttcatgaga tacctgaact tcacggcaca ggtccgccat
     8821 aaacataaat gagccacgca gcagacccac cagcaccata tcgctgccgc tgtctttgta
     8881 acgctcagta atctgacgac ccagttcggc gatacgcgct ttaatctccg cttcggggat
     8941 cattacttct acagtatgtt tcatatctct aaccatatga tttaaaaaca aatcacttcg
     9001 cacgggcagg ttttggcacc gcaacaaagc gcaagacacg cagtatacca gtaaaactat
     9061 tcattcgcgg aatctgttaa taaagctcga attgtgatga cggtcacaca tgttaaaacc
     9121 tataattaat tgctactaaa atattaatga attgaaatgg tgtctcttta tggcaattaa
     9181 caatacaggc tcgcgacgat tactcgtcac gctaacagcc ctttttgcag cgctttgcgg
     9241 gctgtatcta ctcattggcg gaggctggct ggtcgcgatt ggcggctcct ggtactaccc
     9301 tatcgctggc cttgtgatgc tcggcgtcgc ctggatgctg tggtgcagta aacgcgccgc
     9361 gctttggcta tacgcagccc tgctgctcgg caccatgatt tggggcgtct gggaagttgg
     9421 tttcgacttc tgggcgctga ctccgcgcag cgatattctg gtcttcttcg gcatctggct
     9481 gatcctgccg tttgtctggc gtcgcctggt cattcctgcc agcggcgcag ttgccgcact
     9541 ggtggtggca ctgctgatta gcggtagtat cctgacctgg gccggattta acgatccgca
     9601 ggaaatcaac ggcaccttaa gcgccgatgc cacacctgcg gaagctatct cacccgttgc
     9661 cgatcaggac tggcctgcct atggtcgcaa tcaggaaggt caacgctttt cacctctgaa
     9721 acaaattaac gccgataacg tccataatct gaaagaagcc tgggtgttcc gtactggcga
     9781 tgtgaagcag ccgaacgatc cgggcgaaat caccaatgaa gtgacgccga ttaaagtggg
     9841 cgacaccctt tacctgtgta ccgctcacca gcgcctgttt gcgctcgatg ccgccagcgg
     9901 caaagagaaa tggcattacg atcctgagct gaaaactaac gagtctttcc agcacgtaac
     9961 ctgccgtggc gtctcttatc atgaagccaa agcagaaacg gcttcgccgg aagtgatggc
    10021 ggattgcccg cgtcgtatca ttcttccggt caatgatggt cgactgattg cgattaacgc
    10081 tgaaaacggc aaactgtgcg aaaccttcgc caataaaggc gtgctcaatc tgcaaagcaa
    10141 tatgccagac accaaaccgg gtctgtatga accgacttcg ccaccgatta tcaccgataa
    10201 aaccatcgtg atggccggtt cagttaccga taacttctca acccgcgaaa cgtctggcgt
    10261 gatccgtggt tttgatgtca acaccgggga gctgctgtgg gcttttgatc ccggcgcgaa
    10321 agatccgaac gcaatcccgt ctgacgaaca cacctttacc tttaactcgc caaactcctg
    10381 ggcaccagcg gcctatgacg cgaagctgga tctggtctat ctgccgatgg gcgtgaccac
    10441 gccggatatc tggggcggta accgcacacc ggaacaggaa cgttatgcca gctcgattct
    10501 ggcgctgaat gccactaccg ggaaactggc gtggagctac cagaccgttc accacgacct
    10561 gtgggacatg gatcttccgg cacagccgac gctggcggac atcaccgtta atggtcagaa
    10621 agtgccagtt atttacgctc cggcgaaaac cggcaacatt tttgtgctcg atcgtcgcaa
    10681 tggcgaactg gtggttccgg ccccggaaaa accggtgccg caaggtgctg cgaaaggcga
    10741 ttacgttact ccgacccagc cgttctctga actgagcttc cgtccgaaga aagatttgag
    10801 cggtgcggat atgtggggag ccaccatgtt tgaccaactg gtgtgccgcg tgatgttcca
    10861 ccagatgcgc tatgaaggca ttttcacgcc gccatctgaa cagggcacgc tggtcttccc
    10921 gggtaacctg ggaatgtttg aatggggcgg gatttccgtt gatccaaatc gtgaagtggc
    10981 aattgccaac ccaatggcac tgccgtttgt ttcgaaactg atcccacgcg gtccgggtaa
    11041 cccgatggag cagccgaaag atgccaaagg cacgggtacg gaatccggca ttcagccaca
    11101 gtacggtgta ccgtatggtg tcacgctcaa cccgttcctc tcaccgtttg gtctgccgtg
    11161 taaacagcca gcatggggtt atatctcggc gctggatctg aaaatcaatg aagtggtgtg
    11221 gaagaaacgt attggtacgc cgcaagacag tatgccgttc ccgatgcctg taccggtgcc
    11281 gttcaatatg ggtatgccga tgctgggcgg gccaatctcc acggcgggta acgtgctgtt
    11341 tatcgctgct acggcagata actacctgcg cgcttacaac atgagcaacg gtgaaaaact
    11401 gtggcagggc cgtttaccag cgggtggtca ggcgacgcca atgacctatg aagtgaatgg
    11461 caagcagtat gtggtgatct ccgcaggcgg tcacggttca tttggtacga agatgggcga
    11521 ctatattgtg gcttatgccc tgccggatga tgtgaaataa ccataatgcc cggagagatc
    11581 cgggcatatt tccgaatacg gtctttttat accgtaaacc ctaacatcat ccccgtatct
    11641 tcatgctcca gcagatggca gtgcgccata taagcatgtt ctttcggtgc gttgtgatta
    11701 aacttcacca gcacttcact gacattgcct tctaccttaa cggtatcttt ccagcccgcg
    11761 cgatgagccg ctggcggttt gccattttct gacaagatac ggaactgcat gccgtgaata
    11821 tggaacggat gcagcatcat gtcgcccacg ccagagataa cccaacgttc gtactgccct
    11881 ttcgccgccg caaacatcgg cttattcata tcaaacgcct gaccgttgat tttattggca
    11941 tggtggaaat cgaacttccc gccgtggttc atatgattca tattgccgtg ccccatatgg
    12001 cccatcatct ggctgtgatc cattccggcc atcgcctgat cgccatattt ctccattagc
    12061 atctgcatcc ccatcatatc gagcatcggg tccatagaaa gttgcagctt gcgtaccgtc
    12121 agcccttcca gcgaaggtaa cgcaggcagg ctacttaatg tgtcaggcaa agcaccggag
    12181 gcactaatag caatcggctg aatccgcatt accggatgcg gcttgtcaaa cggcgcaatc
    12241 gccatcccca tctggctgac cggtagcgtc accagatcaa agggtttgtt gtcgttaacc
    12301 tccaccagca cttcaaaacg ctcgcccatc agcaccggca gctcgctcac cttcaccggt
    12361 tcaggtagca gaccaccgtc gctggcaatc acatacagcg ggcgattgtc gctggtggcg
    12421 aaattgagcg agcgggcgtt acagccattg agcaaacgca ggcgcagcca gccacgcggg
    12481 gcagcgtgtt gtgggtagat tgcaccgttg gtcagcagcg tatcgccaaa ccagcccacg
    12541 gcggcggtca tcacatccag ttgataatca atctgcccat cagcgttaaa ttttttatcc
    12601 tgaacaatca ccggaacatc atcgataccc cactgttttg gcagcattaa tttcaggatc
    12661 tcgtcatctt caattaccac cagcccagcc aaccccatcg ccacctgtcg cccggtcttg
    12721 ccatgttgat gtggatggaa ccagcaggta gcggcaggtt gatcaacgtt caacgtcacc
    12781 gtacgtttgc cgcctggcgg aataattccc tgcgggccgc cgtcgacgtc acccggtact
    12841 tccagcccgt gccagtgcaa cgtcgtctct tccgccagtt ggttatagat atcaaccgtt
    12901 accgctttgc cgcgctgtaa tttcaccgcc ggccccagca gattgccgtt atagccccag
    12961 gtagttgcag ttttctcgcc aaaggtagac tgacctgcgc caatagttaa ctgaatgcga
    13021 ttacgggcat cggtcgtgag caaatcaggg atcggtaacg ttgggcgttc cgccgcaaag
    13081 actgcgcggc tccacagcgg caaggctgaa gccacaccca gcgcgacgga atattttaag
    13141 aaatcacgac gttgcatagt tatttcctta ttcttaagca ggcgaaacaa aatcaaacgt
    13201 tgagcatagt ccttcccctt acgggaaggt caagccgcag acacaattta atcgacaata
    13261 atggtcgtcg cctcgctcgc agtgtgctaa cgtttatctt ctttaagccc ctggtagaag
    13321 caatgaagac gtttttcaga acagtgttat tcggcagcct gatggccgtc tgcgcaaaca
    13381 gttacgcgct cagcgagtct gaagccgaag atatggccga tttaacggca gtttttgtct
    13441 ttctgaagaa cgattgtggt taccagaact tacctaacgg gcaaattcgt cgcgcactgg
    13501 tctttttcgc ccagcaaaac cagtgggatc tcagtaatta cgacaccttc gacatgaaag
    13561 ccctcggtga agacagctac cgcgatctca gcggcattgg cattcccgtc gctaaaaagt
    13621 gcaaagccct ggcccgcgat tccttaagcc tgcttgccta cgtcaaataa tccctgatac
    13681 ctttttgtag aaatattgac cgtgcatccg cggtcaatgt tagctattat gttgcgccct
    13741 ttttttacgg gtgttaacaa aggaggtatc aacccatggc cgaaaaaaaa cagtggcatg
    13801 aaacgctaca cgaccagttt gggcagtact ttgcggtaga taacgttctg tatcatgaaa
    13861 agaccgatca ccaagatctg atcatttttg agaacgctgc atttggtcgc gtaatggcgc
    13921 tggatggcgt agtacaaacc accgagcgcg acgagtttat ctatcatgag atgatgaccc
    13981 atgttccgct actggcccac ggtcacgcga aacatgtgct gattatcggc ggcggcgacg
    14041 gtgccatgct gcgtgaagta acccgacata aaaacgttga gtcaatcacg atggtggaaa
    14101 tcgatgcggg tatcgtgtcg ttctgccgtc agtatctgcc caaccataac gccggtagct
    14161 acgacgatcc gcgctttaag ctggtgatcg acgatggcgt caatttcgtt aatcaaacca
    14221 gccagacctt tgatgtcatt atctccgact gcaccgatcc tatcggtccc ggcgaaagtc
    14281 tgttcacctc agcattttat gaaggctgca aacgttgcct gaatcctggc ggtatcttcg
    14341 tcgcacaaaa cggcgtctgc tttttacagc aggaagaagc catcgacagc tatcgcaaac
    14401 tcagccatta cttcagcgac gttggctttt atcaggcggc gatcccgacc tattacggcg
    14461 gtatcatgac ttttgcatgg gcgacagata acgacgcctt acgccatctc tcaaccgaaa
    14521 ttattcaggc gcgttttctc gcttctggcc tgaaatgccg ttattacaat ccggcaatcc
    14581 atacggcagc ttttgcctta cctcagtatc tgcaagacgc actggcttca cagccgtcct
    14641 aaggagaaga taagaaattg aaaaaactga aactgcatgg ctttaataat ctgaccaaaa
    14701 gtctgagttt ttgtatttac gatatctgct acgccaaaac tgccgaagag cgcgacggtt
    14761 atattgctta tatcgatgaa ctctataatg ccaaccgtct gaccgaaatc ctgtcagaaa
    14821 cctgttccat tatcggggcc aatattctta acatcgcccg ccaggattac gaaccacagg
    14881 gtgccagcgt cactattctg gtgagtgaag aaccggttga cccgaaactc atcgacaaaa
    14941 cagaacaccc cggcccactg ccagaaacgg tcgttgccca tctcgataaa agtcatattt
    15001 gcgtacatac ctacccggaa agtcatcctg aaggcggttt atgtaccttc cgcgccgata
    15061 ttgaagtctc tacctgcggc gtgatttctc cgctgaaggc gctgaattac ctgatccacc
    15121 agcttgagtc cgatatcgta accattgatt atcgcgtgcg cggttttacc cgcgacatta
    15181 acggtatgaa gcactttatc gaccatgaga ttaattcgat tcagaacttt atgtctgacg
    15241 atatgaaggc gctgtatgac atggtggatg tgaacgtcta tcaggaaaat atcttccata
    15301 ccaagatgtt gcttaaagag ttcgacctta agcactacat gttccacacc aaaccggaag
    15361 atttaaccga cagcgagcgc caggaaatta ccgctgcgct gtggaaagaa atgcgcgaga
    15421 tttattacgg gcgcaatatg ccagctgttt aacggctctg gcggagctcc caggctccgc
    15481 cagatttatt tacttctgct gcacgaaatt gcggtaagcc gccacgacct gcagaaaatc
    15541 ctcaacgccg catagcgaca ggctttcttc gtcgtagtag ttcatccctt cttccatttc
    15601 atcgccagcg aattccagtt gattggcgcg aaccatcacc tcttcaccgt ccatccacag
    15661 ggtgtattca tgccctgccc gttgccagga ccgttcgcta cctttcagtg cgtgcgctgc
    15721 ttgttccact tcatcaagca aagccaggtt ttctttcacc tcttcattaa accagtgccc
    15781 gaccacttca tgccccatgg acatacgcac ctttaccact ccggtaatat cgcgcagaaa
    15841 ttcgtaatcc atagtgtgtt cctctgctcc cggcaatgcg ccgaaatcgc attgcgctat
    15901 tgatgtaatt attgcagcct taccggagaa aaacagcgga acggcgatgg tttagaaata
    15961 aaaaatgccc gggaatctca ttcaccgggc attgtgtcgt ttatgcgcag cgcgtgcgct
    16021 tacttattac accgcagtct ggaaaatcac cccatcggct ttttcggtgt actgagaaag
    16081 ctggttgaag ttcagataac gataagtatc aacggcagtt ttatctactt gcgcaacgta
    16141 ggtctggtac tcttccggcg tcggcagttt gccaatcagc gccgcaacag ccgccagttc
    16201 tgcagaagcc aggaagacat tcgcgccagt ccccagacgg ttcgggaagt tacgggtaga
    16261 ggtggaaacc accgtcgcac cgtctgctac acgcgcctgg ttacccatac acagagaaca
    16321 gcccgggatc tcgatacgag ccccgctctt accgaagacg ctgtagtagc cttcttcagt
    16381 caactgtgcg gcgtccatgc gggttggcgg tgccacccac aggcgggtcg gcaactggcc
    16441 tttgtgcgca tccagcagtt tacccgcagc acggaagtga ccgatgttag tcatgcaaga
    16501 accgataaac acttcgtcga tcttctcgcc ctgtaccgca gacagcggac gtgcgtcgtc
    16561 cgggtcgttc ggtgcacaga ggattggctc tttaatatcc gccagatcga tgtcgatcac
    16621 tgccgcgtat tcagcatctg catcggcttc cagcagctca ggattcgcca gccatttttc
    16681 catgccctga atacgacgtt cgagggtacg acgatcgcca taaccttccg cgatcatcca
    16741 cttcagcagg acgatgttag agttcaggta ttcgatgatc ggttctttgt tcagcttgat
    16801 agtacaacca gcggcagaac gctcagcgga cgcatcggtc agttcaaatg cctgctcaat
    16861 tttcagatcc ggcagacctt caatttccag aatgcgacca gagaagatgt ttttcttgcc
    16921 tttcttctca acggtcagca gaccttgttt gatcgcatac agcgggatcg cgtgtaccag
    16981 atcgcgcagg gtgatgcccg gctgcatttt gcctttgaag cgcaccagaa cggattccgg
    17041 catatccagc ggcattacgc ctgttgcggc tgcaaatgcc accagaccag aacccgccgg
    17101 gaaagagata ccgatcggga aacgggtatg ggagtcacca ccggtaccga cggtatccgg
    17161 cagcagcata cggttcagcc aggagtggat tacgccgtca cccggacgca gcgacacacc
    17221 accacggttc ataatgaagt ccggcagcgt gtggtgcgtg ttcacgtcaa ctggcttcgg
    17281 atacgccgcg gtatggcaga aagactgcat caccaggtca gccgagaagc ccaggcacgc
    17341 caggtctttc agttcatcac gggtcatcgg gccggtggtg tcctgtgaac ctacagaagt
    17401 cattttcggt tcgcagtacg cgcccggacg aatacctttc acaccgcagg cacgacctac
    17461 cattttctga gccagcgaga agccgcgatc gctctcagcg acatctttcg cctgacggaa
    17521 cacatcactg tgcggcagac caagtgcttc acgcgctttg gtggtcaggc cacgcccgat
    17581 aatcagcgga atacggccac cagcacgcac ttcatcaatc agcacgtcgg ttttcagttc
    17641 gaaggtcgcc agcagttcgc cggtttcgtg gttacgcact tcacctttgt acgggtaaac
    17701 gtcaatcacg tcgcccatgt tcaggttaga gacgtcgact tcgattggca gtgcacccgc
    17761 gtcttccatc gtgttaaaga agatgggtgc aattttaccg ccgaggcaca aaccaccgcc
    17821 gcgtttgttc ggcacatgtg gaatatcatc gcccataaac cacagaacgg agttcgtggc
    17881 ggatttacgc gatgaacccg tacccacaac gtcaccgacg tacgccagcg ggaaaccttt
    17941 ctgttgcaga gcttcgattt gcttgatcgg gccaacaaca cctggctggt ctggctcaat
    18001 accttcacgg gcgtttttca gcatcgccag cgcgtgcagt gggatatccg ggcgcgacca
    18061 cgcatccggt gccggagaaa ggtcatcagt gttggtttcg ccagtgactt tgaagacggt
    18121 aacggtcagt ttttcagcca gcgccgggcg attcaggaac cattcggcat ccgcccagga
    18181 ctgcattacc tgcttcgcat attcgttgcc tgctttggct ttctcttcta cgtcatagaa
    18241 gttatcgaac atcagcagtg tgtgagaaag tgctttggca gcgatcggtg ccagtttggc
    18301 atcatccagc gcgtcgatca gcggatgaat gttgtaacca ccctgcatgg tacccagcag
    18361 ttcgatggct ttttccggag tcagcagagg ggatttggct tcgcctttag cgatagcagc
    18421 caggaagcct gctttgacat aggcggcttc atcgacgcca gggggaacac ggttggttaa
    18481 tagatctaac aggaattctt cttcgcccgc gggcgggttt ttcagcagct ctacaagtgc
    18541 ggccatttgg tttgcatcca ggggtttggg cgcaatcccc tcagcggcac gctcagctac
    18601 gtgcttacgg tattcttcta gcacgacggt tctcctcgct ctcattgtca tagtgcggca
    18661 ggcgattctc ttcacgctcc tgtgagacag cagtttgtag ggtaaatgcc cagtaccgca
    18721 acgggcagaa tagcaggatt ttgacgacgt gttaatctgt ttacaaaaaa gcaacataaa
    18781 aaagtggctg aatcgttaag gatggtctag agattgtttc cctccgtaaa aacttcgtta
    18841 attcgcatgg tgataatcac ctttctcaac gaatcccaag attagtcaaa atttaaacta
    18901 ccgcctcttt atactcggat taacagcacc tgcgggtggc agttcgccga ccattgcgat
    18961 ttccttgaga tccgaattat gaaaatgact ttgccgttta aaccccatgt gctggcacta
    19021 atttgcagtg ccgggctttg tgccgcctct gccgggctat atataaaaag ccgcacagtg
    19081 gaagcgcctg tggaaacgca atcgacacaa cagactgcgc ctgacatcac cgcagttacg
    19141 cttcctgcaa cggtttccgc accgcccgta acgcccgccg tcgtcaaatc cgcattcagc
    19201 actgcacaaa tcgatcaatg ggttgcgcct gtcgcgctgt accccgattc tctgctttca
    19261 caagtgttaa tggcatcaac ctatccggca aacgttgctc aagcagtgca atggtcgcac
    19321 gataatccac ttaaacaagg cgatgctgct attcaggcgg tatctgacca gccgtgggac
    19381 gccagcgtta aatcactggt ggcctttcca caattgatgg cattgatggg cgaaaacccg
    19441 caatgggtgc aaaacctggg cgatgctttt ctggcgcagc cgcaggacgt gatggactcg
    19501 gtacagcgat tgcggcaact ggcgcaacaa accggctcgc tgaagtcatc aaccgaacag
    19561 aaagttatta ccacaacgaa gaaagctgta ccggtaaaac agacagtcac ggcacccgtc
    19621 ataccatcca ataccgtttt aactgccaac cccgtcatta cagagccggc aacaaccgtc
    19681 atttccattg agcccgccaa tcctgatgtg gtctatattc ccaactacaa cccaaccgtg
    19741 gtttacggga actgggccaa tactgcgtat ccgccggttt atctgccacc accagccgga
    19801 gaaccgtttg ttgacagctt tgtgcgcgga ttcggctata gcatgggcgt tgctaccacg
    19861 tacgcactat ttagcagcat cgactgggat gacgacgatc atgaccatca tcatcatgac
    19921 gatgatgatt atcatcacca cgatggcggt catcgtgacg gtaatggctg gcaacacaac
    19981 ggcgacaaca tcaatatcga cgtcaacaat ttcaaccgta tcaccggtga gcatcttact
    20041 gataagaata tggcatggcg gcacaatcca aactaccgta atggtgtgcc ctatcatgat
    20101 caggatatgg caaagcggtt tcatcaaacc gatgtcaacg gcggaatgag tgccacgcag
    20161 ctacctgctc caacacgcga cagccagcgt caggcggcag caagtcagtt tcagcaacga
    20221 acacacgccg cccccgtcat tacacgagat acccaacgtc aggcagcggc acagcggttt
    20281 aatgaagctg aacactatgg gagctatgac gacttccgcg acttcagccg tcgccaacca
    20341 ctgacccagc aacaaaagga cgccgctcgt cagcgttatc aatcggcctc tcctgagcag
    20401 cgccaggcgg ttcgcgagaa aatgcagact aacccacaga accagcagcg aagagaggca
    20461 gcgcgtgagc gtattcagtc tgcaactccc gaacagcgcc aggtgtttaa ggaaaaagta
    20521 cagcagcgcc cactgaacca acagcaacgt gataacgccc gccagcgtat acaatcggca
    20581 tcacctgaac aacgtcaggt ttttcgggaa aaagttcagg agagccgccc acaacgtcta
    20641 aacgacagta accatactgt caggctgaat aacgagcaac ggtcagcagt acgcgaacgt
    20701 ctctctgagc gcggagcaag gcgacaggaa aggtaaattg cagacgtaaa aaaagcggcg
    20761 tggttagccg cttttttaat tgccggatgt tccggcaaac gaaaaattac ttcttcttcg
    20821 ctttcgggtt cggcaggtcg gtaatgctac cttcgaacac ttctgccgcc aggcccacag
    20881 actcgtgcag agtcgggtgc gcgtggatgg tcagtgcgat gtcttcagca tcacaaccca
    20941 tttcgattgc cagaccgatt tcacccagca gctcaccgcc gttagtaccg acaatcgcac
    21001 caccgatcac acggtgagat tctttgtcga aaatcagctt ggtcataccg tctgcgcagt
    21061 cggaagcgat agcacgacca gaagcagccc acgggaaggt ggcggtttca tagctgatgc
    21121 ctttctcttt cgcttctttc tcagtcagac ctacccatgc aacttctggt tcggtatagg
    21181 cgatagacgg gataactttc ggatcgaagt agtgtttttt accggcgata acttcagcgg
    21241 caacgtgacc ttcgtgaaca cctttgtgtg ccagcatcgg ctgaccaacg atatcgccga
    21301 tagcaaagat gtgcggtacg ttggtacgca gctgtttgtc aacgcggatg aaaccacggt
    21361 cgtccacttc cacgccagct ttgcctgcgt cgaggttttt accgttcggc acacgaccaa
    21421 tcgctaccag cacggcgtcg taacgctgcg gttcagcggg tgcttttttg ccttccatcg
    21481 tcacataaat accgtcttct ttcgcttcaa cggcggtaac tttggtttcc agcatcaggt
    21541 tgaatttctt gctgatacgc ttggtgaaga ctttaacgat gtctttgtca gctgccggga
    21601 taacctggtc gaacatttca accacgtcaa tctgtgaacc cagcgcgtgg tatacggtgc
    21661 ccatttccag accgatgata ccgccaccca ttaccagcag gcgttctggt acttctttca
    21721 gttccagcgc gtcagtggag tcccagatac gcggatcttc atgcggaata aacggcagtt
    21781 ggatcgggcg agaacccgct gcaatgatcg cattgtcgaa gttgatcacg gttttgccgt
    21841 tctcaccttc aacttccagg gtgttagccc cggtgaattt acccagaccg ttgaccactt
    21901 tgactttgcg gcctttcgcc ataccagcca gaccaccggt cagctgattg atcactttct
    21961 ctttccaagt acgaatcttg tcaatatcgg ttttcggttc gccgaagacg ataccgtgtt
    22021 cagccagcgc tttggcttct tcgataactt ttgctacgtg cagcagtgct ttagaaggga
    22081 tacagccgac gttcaggcaa acaccgccaa gggtgttgta acgttctacg attacggttt
    22141 ccagacctaa atcagcgcaa cggaaggcag cggagtaacc tgcggggcct gccccaagta
    22201 ccacgacctg agttttgatt tcagtactca tcatgacctc tatatattta tctccggcgg
    22261 tcatacccgt cgtctttcag gccgcagcgg cgttagcttc acgccctcac cccagtcact
    22321 tacttatgta agctcctggg gattctggcg cttgctacct tgctgcaccc agaaatccat
    22381 agggtatgtg aagtaatacc ctaaccacca ccgggtcgtt ctatccgtcg gtattttaca
    22441 aaattgttaa caatttttaa acaacaaacg gcaaccgatt tgtctattcg ctaataacct
    22501 caatacattc a
//
LOCUS       DAZPAC010000052        21642 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZPAC010000052 DAZPAC010000000
VERSION     DAZPAC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.052"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="ACHUPM_001686"
     CDS             complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="ACHUPM_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR0869490.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPRSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1344..2996
                     /gene="ushA"
                     /locus_tag="ACHUPM_001687"
     CDS             1344..2996
                     /gene="ushA"
                     /locus_tag="ACHUPM_001687"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR0869491.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="ACHUPM_001688"
     CDS             complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="ACHUPM_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR0869492.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3716..4510)
                     /locus_tag="ACHUPM_001689"
     CDS             complement(3716..4510)
                     /locus_tag="ACHUPM_001689"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR0869493.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4648..4989
                     /locus_tag="ACHUPM_001690"
     CDS             4648..4989
                     /locus_tag="ACHUPM_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0869494.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5204..7708)
                     /gene="copA"
                     /locus_tag="ACHUPM_001691"
     CDS             complement(5204..7708)
                     /gene="copA"
                     /locus_tag="ACHUPM_001691"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR0869495.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7970..8902
                     /gene="glsA"
                     /locus_tag="ACHUPM_001692"
     CDS             7970..8902
                     /gene="glsA"
                     /locus_tag="ACHUPM_001692"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR0869496.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8905..10197
                     /locus_tag="ACHUPM_001693"
     CDS             8905..10197
                     /locus_tag="ACHUPM_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR0869497.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10228..10325
                     /locus_tag="ACHUPM_001694"
                     /pseudo
     CDS             10228..10325
                     /locus_tag="ACHUPM_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10322..10729
                     /gene="cueR"
                     /locus_tag="ACHUPM_001695"
     CDS             10322..10729
                     /gene="cueR"
                     /locus_tag="ACHUPM_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR0869498.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10730..11188)
                     /locus_tag="ACHUPM_001696"
     CDS             complement(10730..11188)
                     /locus_tag="ACHUPM_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR0869499.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11185..12102)
                     /locus_tag="ACHUPM_001697"
     CDS             complement(11185..12102)
                     /locus_tag="ACHUPM_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR0869500.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12248..12925
                     /gene="fetA"
                     /locus_tag="ACHUPM_001698"
     CDS             12248..12925
                     /gene="fetA"
                     /locus_tag="ACHUPM_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR0869501.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12912..13691
                     /gene="fetB"
                     /locus_tag="ACHUPM_001699"
     CDS             12912..13691
                     /gene="fetB"
                     /locus_tag="ACHUPM_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR0869502.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="ACHUPM_001700"
     CDS             complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="ACHUPM_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR0869503.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="ACHUPM_001701"
     CDS             complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="ACHUPM_001701"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0869504.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="ACHUPM_001702"
     CDS             complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="ACHUPM_001702"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFR0869505.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16062..16748
                     /gene="ybbA"
                     /locus_tag="ACHUPM_001703"
     CDS             16062..16748
                     /gene="ybbA"
                     /locus_tag="ACHUPM_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR0869506.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16745..19159
                     /gene="ybbP"
                     /locus_tag="ACHUPM_001704"
     CDS             16745..19159
                     /gene="ybbP"
                     /locus_tag="ACHUPM_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFR0869507.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="ACHUPM_001705"
     CDS             complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="ACHUPM_001705"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR0869508.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20556..21482)
                     /gene="allS"
                     /locus_tag="ACHUPM_001706"
                     /pseudo
     CDS             complement(20556..21482)
                     /gene="allS"
                     /locus_tag="ACHUPM_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5015 a         5684 c         5799 g         5144 t
ORIGIN      
        1 gatcggcgat aagccccaga actgccgctc ccagacctcc catgccaaaa gcaaaaccga
       61 aaaagagtcc agaaaccata ccgatacgtc ccggaagtag ctcctgagcg tagaccagaa
      121 tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc cccgtccagt
      181 gcaggctggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata gagccccaaa
      241 tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta cctgccgcaa
      301 cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat ccgaattttt
      361 gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat ttcgagaaaa
      421 tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt ggattgatta
      481 tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa cggctaattt
      541 gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg ttgcctttgc
      601 cataaggcgc gataatcacc gccgccagca agggtcccag ggaactgcca aagttgccgc
      661 cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata cgggctacgc
      721 gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc gccagcagaa
      781 ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta aagcacatgc
      841 caattggcaa cgaccgcggc atcggatatt tatcggtcca gtagccgacc actggttgca
      901 gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata aatgtcagag
      961 aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc atgtcgttca
     1021 gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc ttggtcgttg
     1081 acgcagccgc gcccgccaca ggctggggtt gttcactcat tgccatagga aagtcacttt
     1141 ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact aacatacctg
     1201 tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct ggatgcagat
     1261 ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt tcaacacgtt
     1321 gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg ttagcgctgt
     1381 taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat aaaacctaca
     1441 aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat gaatatggcg
     1501 aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag gttgcggctg
     1561 aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg cccgagtctg
     1621 acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat gacgcgatgg
     1681 cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag gaaaagtggg
     1741 ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag cgcctgttta
     1801 aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg ctgacaaccg
     1861 atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt cgtaagcccg
     1921 ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca gacattatta
     1981 tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac gcaccgggcg
     2041 atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc ggtggtcact
     2101 cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac gtgccgggta
     2161 cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat gagtggggca
     2221 aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg gttaactacc
     2281 agctgattcc ggtgaacctg aagaagaaag tgaccaggga agacgggaaa agcgagcgcg
     2341 tgctgtacac ccctgaaatc gctgaaaacc agcaaatgat ctcgctgtta tcgccgttcc
     2401 agaacaaagg caaagcgcag ttggaagtga aaataggcga aaccaatggt cgtctggaag
     2461 gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt ctggcagccc
     2521 aaatggatcg cactggtgcc gactttgcgg tgatgagtgg aggaggaatt cgtgattcta
     2581 tcgaagcagg cgatatcagc tataaaaacg tactgaaagt gcagccattc ggcaatgtgg
     2641 tggtgtatgc cgacatgatc ggtaaagagg tgattgatta cctgaccgcc gtcgcacaaa
     2701 tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg gcgaaagacg
     2761 gcaaactcaa cgacctgaaa atcaaaggcg aaccggtcga tccggcgaaa acttaccgta
     2821 tggcgacatt aaacttcaat gccaccggcg gtgatggcta tccgcgcctt gataacaaac
     2881 cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat atccagaaaa
     2941 gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg cagtaatccg
     3001 aaagtgccgg atatttgcat ccggcacaat gcttaatcgc ggcgggcgat atcagcaaat
     3061 ttggcatcga ggatcttcgc cagatcgcct gccgccagtt cgatatccag tccgcgcttg
     3121 ccgccagaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat cgtcggcaga
     3181 cgttttttct gccccaatgg gctaatcccc ccaaccaggt atcccgtcga acgctgcgcg
     3241 gccatcggat cggccatctc aactttcttg gcacccagcg cttttgctac ttttttaaga
     3301 tccagttgac ctgcgaccgg cgtaacggcc actgccagat gtttcatatc accgttcact
     3361 gccaccagta gcgttttgta gacttgatcc ggattcaaac ctaatttttt gacgacttca
     3421 tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa cgaaatcttg
     3481 tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta aacaaaaaaa
     3541 cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc taagagtatt
     3601 ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt gacggcataa
     3661 taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca tttttttagc
     3721 gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc gcgaccacat
     3781 atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc tgatgcatca
     3841 gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta ttttgcggag
     3901 gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta tgccagtggg
     3961 tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac atggtaatct
     4021 ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc gcctgcaata
     4081 actggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc gtcgcctgaa
     4141 gaaccatcgc gatttgccac agcggttgag tagaaaagag cgacggagaa atgcccatct
     4201 cctggctaat gtgctgcaga ttttggagtt gctcctcgtt aatgcgctct tccagcgcct
     4261 cgcaagtagg caaattagca aaaggcgtat cgctggtgga aacatccgcc tcgacgatca
     4321 gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc atatcgtggc
     4381 tacccatatg aatactgcca atcagatgaa aatggcgatt gccgggaagg gtgatatcga
     4441 tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt acccggtaca
     4501 acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga gggatggcgc
     4561 aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct tgcatcgcta
     4621 ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc gacgacaccc
     4681 ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa tgagttagca
     4741 gagttgctgc atgttcatcg taatagcgtc agtgcactga tcaataacaa tcgtaaactc
     4801 accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga ttttaggcta
     4861 aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac ccaggaagaa
     4921 ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg tgcaaaaaag
     4981 gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt cacgccgcat
     5041 ccggcatccg agcaccattg cctgatgcga cgcttacccg tcttatcagg cctacaaacc
     5101 tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc atgcgtcgat
     5161 gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc ttcggtttaa
     5221 accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc attgccgctc
     5281 cggcaactac cgggttaagc agtgttcccg taaacggcca caaaataccg gcggcgaccg
     5341 gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg ttgcgcagcg
     5401 ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc atcagggtaa
     5461 tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg acatccgcct
     5521 gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga cgtccttcac
     5581 tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca atcacttcat
     5641 caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg gtcaacatca
     5701 ccagacgata tcccgtttta tgcaggcgtt gcagcgccgc cacgctatca ctacgcaacg
     5761 gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc acaggcgttg
     5821 ctccttgcga tgcctgagca gtaatctccg cttcgatagc tttggtacca acctgttgct
     5881 catttaacag cgcctgattg cccagcaata acgcatgacc ttcagcttca ccgctcacgc
     5941 ccagcccgcg caatgtgcgg aaaccgttga cctgcggcag ctgcatatca cctgctttat
     6001 cgaggatcgc ccgtgccagc ggatggctgg aaccttgctc cagtgccgcc gccagacgca
     6061 atgcctgcgc ttcatcaaca tcagcaaacg ttttcactgc gacaacctgc ggcttccctt
     6121 cggtcagcgt cccagtttta tcgaacacga tggtatcgag tgtactggcg cgttgcagcg
     6181 cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg gaaataatcg
     6241 acatcggcgt cgccagcccc agcgcacacg gacaggcgat aatcagtacc gtggtggcaa
     6301 tcaccagggt ataaacaatt tgcggtgccg gaccaaagaa gtaccagatt gccgcactga
     6361 ccagcgcaat caccaccacc acaggtacaa atacggctga gattttatcc gccagctgac
     6421 cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatccgt gacagcgtgg
     6481 tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc actgtcccgg
     6541 catggacgct ttcgccttcg cctttttgct gtgggattgg ttcgcccgtc agcatcgctt
     6601 catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga tcgccggtcg
     6661 tcaggcgcag taacatccct ggctgaactt ccgccagcgg cacgcttttt tcaccatctg
     6721 cggtgaccac tcgcgccgtc ggtggggtta aatcgagtaa tttctccagc gccttagaag
     6781 aacgctgacg tgcgcgcgct tccagcatat ggccgagatt aatcagaccg ataatcatcg
     6841 cactggcttc atagtaaaga tgccgcgctt ccatcgggaa ccactgcggc cacaggttga
     6901 cgctcatcga atagagccac gccacacccg ttcccagcgc caccagcgta tccatcgtcg
     6961 ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg gcgaaaacca
     7021 tcactgccag ggttatcagg ccgataacta accacaagct gcggttgtca gcggtgacca
     7081 tcatgttatc gccgatcatc ccccagacca ttaccgggat acccacagcc agtgcaacaa
     7141 ttgcctgcca gcggaagcgc ttcatcgtag ctacggcggt ttcttgctgg cgctcgcggc
     7201 gtttagcgtc atcttcaatc gcttccgcac cgtagcccgc tttttccacc gcctgcacta
     7261 aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg tttacccgtg
     7321 cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca cagctggcgc
     7381 agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc gctgccggaa
     7441 gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc agcggtttag
     7501 cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg atcagctgtt
     7561 ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgcc tgctcaacat
     7621 ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag gacaggccgt
     7681 ccagggtcag gtcgatagtt tgtgacataa aacactcctt taagacagtt ttgactggct
     7741 gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa ccaggcgggt
     7801 aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac atttatttct
     7861 ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg aaaatcggca
     7921 ccggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga tgttagatgc
     7981 aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac ttaacggcgg
     8041 acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg cggcagtggc
     8101 tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc gctttgcact
     8161 ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg gcccgcaggc
     8221 ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag ttatcgcctt
     8281 agagttgcat ggcggcaaac cgctgtcgcc actggtaaat gctggcgcta ttgccaccac
     8341 cagcctgatt aacgctgaaa atgttgaaca acgctggcag cgaattttac atatccaaca
     8401 gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg aacaaacaac
     8461 caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc tctattgtga
     8521 tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca atactgttga
     8581 actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga cgcataaacg
     8641 cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg aagggctgta
     8701 tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg gtgtaggtgg
     8761 cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac caccgctgga
     8821 cgaagaaggc aacagtgttc gcggtcaaaa aatggtggca tcggtcgcta agcaactcgg
     8881 ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg taacaaaccc
     8941 ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc ggggatcttc
     9001 gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctgggtcgc ctttgctttt
     9061 ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc gagctatccc
     9121 agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt cttttcgcta
     9181 gcgctctcgt tgctgtacct gttgacgctg gcggtgagca tcgccatggt tgcccgtgct
     9241 tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca ccttattttg
     9301 ctctacgcgt tggggatcat tgcggtgatg acgcttttta actccttaag caaccatgcg
     9361 gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt attgctgatt
     9421 attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc ccccagctcc
     9481 ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt tggcatgatg
     9541 gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc gtttctggtg
     9601 gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct tagcgatgta
     9661 tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc ttctccgctg
     9721 ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac ggcttcagcc
     9781 attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag cgaacgcgaa
     9841 ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa cattattgtc
     9901 gtggtgttga ttatgctgat gacggcggcg ctaaatttag gctcactcgc cagtgttgcc
     9961 agcgccacct ttttgatttg ctacctggcg gtgtttgtgg tggcgatccg cctgcgtcat
    10021 gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt gttggtgatc
    10081 gttggcttta tctacagtct gtggtcccag ggtagccgtg cgttgatatg gattattggc
    10141 tcactcttac tcagccttat tgtggcaatg gtcatgaagc gcaataaaac cgtataacat
    10201 ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg cactggcaaa
    10261 aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg ttgcgcgaac
    10321 gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca ttcgcttcta
    10381 tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc gcacctacac
    10441 gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg gctttaacct
    10501 ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca gcgccgacgt
    10561 caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg agctgcaatc
    10621 catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca gcgccgactg
    10681 cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat taagacgaga
    10741 cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatgc gtacctgcac
    10801 cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata tgaccgcgcc
    10861 cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc ccgcgctggt
    10921 ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat ttccaccaca
    10981 gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc caacccagcg
    11041 gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac aacaaataac
    11101 catttccgcc cagcatctcg gctgccagca gcaaaccgcc gagactgagc cagaaaatat
    11161 gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc tgtctttcac
    11221 cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg gcatcatcac
    11281 tactttgctg ttactggagg taccgatctg ctgtaacgct tcggtgtatt tctgcgctac
    11341 gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca ttttggtggc
    11401 gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg ccgactgacg
    11461 ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga ggatttccgc
    11521 ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg ctttcatctg
    11581 cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt caatacgggt
    11641 gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc gtgagttgat
    11701 gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg tacggatgtt
    11761 agtcatggtc aggttgatga tcgccagctc cagattgctg acttcataag ccgcgcgcgg
    11821 cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt tatctttcga
    11881 gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct tgcgaccaat
    11941 gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg tatagcgacc
    12001 aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg cgccgacaat
    12061 gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa cctcctgttg
    12121 taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg attactgaaa
    12181 gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc agaaaaaggg
    12241 aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat atctggcggg
    12301 tgatgcgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat ttaagttaat
    12361 taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt cattgatcag
    12421 cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa agccagaaat
    12481 ctaccgccaa caagtctctt actgcgccca gacaccgacg ctgtttggcg atacggtata
    12541 cgataatctg atctttccct ggcagatccg taaccggcag cctgacccag ccatttttct
    12601 cgattttctc gaacgcttcg ccttgccgga cagcattttg acgaaaaata tcgccgagct
    12661 atctggtggt gaaaaacaac gcatctcact gattcgtaac ctgcaattta tgccgaaggt
    12721 tttattgctg gatgaaatca ccagtgcgct ggatgaaagt aataaacata acgttaatga
    12781 gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga cacacgataa
    12841 agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg gagaaatgca
    12901 ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg gcattaatgc
    12961 tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag aaagatattc
    13021 tctggagcgt cggacgagcg ataattcagc tgattattgt cggctatgtg ctgaagtata
    13081 ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc tgctttaatg
    13141 cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc tcatcgttta
    13201 ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca gggtcgattg
    13261 aatttatccc gatgcaggtg atccctatcg ccgggatgat tgccggtaac gccatggtag
    13321 cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag caacagattc
    13381 aggagaaact gagtcttggt gcgacgccga agcaggcttc agcgatattg attcgcgaca
    13441 gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc ttagtgagtt
    13501 taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag gcgattaaat
    13561 atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc attattgcct
    13621 gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg acgcaattga
    13681 agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg ttcatgtgcc
    13741 gatcaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc gacgccagtg
    13801 catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc tgaccgtctg
    13861 cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct tcattgcgcc
    13921 caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct ggattctccg
    13981 ccacctgctg ttgcaactgt tgaatttccg gcgtatcagc cgcctgcttc agcagttcga
    14041 tttgcgccac catcccctgg tagcgggtgt cctgatcctg taacggaatg gttttcagca
    14101 ccgcttctgc atcttcagaa cggttcagcg caatcagcgt ttctgccaac agcaggccga
    14161 tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg gcatcggtga
    14221 aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct tcgcgcggca
    14281 gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg aagccatcta
    14341 ccggttgccc gttctggaac agatacaccg tcggaatcgc gcgcagacca aactgggcgg
    14401 caatcatctg ctccgcatcg cagtccagct tcgccagaat aaattgcccg ttgtactgcg
    14461 ccgcaaggct ttccagaatt ggggttaact gcaaacagtg ctggctacgt tcagaccaaa
    14521 aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg ttagattcgt
    14581 taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat attttctttg
    14641 acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc atcacgcgcc
    14701 ccggcagcag gcgcttaagc accattactg cccaagtcac cagcgttacc ggatagcgca
    14761 tcttcggctt ctcgctaata aaagcatggc gtactttgtc caccaccgct tccggtccca
    14821 acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc gtctggttga
    14881 cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact ttaattccgc
    14941 tgtggcgtag ctccatgcgc agcgcgtctg accatgcctc cagcgcatat ttactggccg
    15001 cgtaagcgcc acgacccggt gtggagatta atcccatcac cgatgatgtc atcacaatac
    15061 gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg tgtgcgccga
    15121 aaaagttggc ggaaaactgc tgttccatct gcgcgcggct gatggtggaa agggggccat
    15181 acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc agggcgatca
    15241 cttcgtcggc tgcgcgatca acactttctg gcgaatccag atcgatcaac acgccggtaa
    15301 atcccatgct gttcatacgc tcaacatcat ccggtttccg gcaacctgcc agcacatgaa
    15361 aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa catccggtaa
    15421 ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta tgagtcatga
    15481 tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg ggcgtcgcgg
    15541 ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc ttccataaaa
    15601 aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc gctaaaggct
    15661 tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa tggttcagcg
    15721 ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg ctgtggctga
    15781 aaaccacgca aaccgtcatt gccgcccagt tcaaccagca cccaacgcgg ctgatgctgt
    15841 ttcagcagag ccggaaggcg cgccagtcct tgttgcgagg tgtcgccgct gatgctggca
    15901 ttaactaccg acgttttact ctgccactta tcattcaaca aggcaggcca ggccgcgctg
    15961 gcagacattc gatacccggc gctcaggcta tcacccagaa tcaataacgt gtccgctgcg
    16021 gcggcacgga aggttaacag ggccaggaac aggaagggca aatgccagcg gaaaacattg
    16081 ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc tccatcctca
    16141 ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc gagtcgggat
    16201 cgggtaagtc aaccttgctg gcgatcctcg ccgggcttga tgacggcagc agtggcgaag
    16261 tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca aagttgcgcg
    16321 cgaagcacgt cggctttgtt tttcagtcat ttatgttaat tcctaccctt aacgcgctgg
    16381 aaaacgtcga gcttccggct ctgctgcgcg gtgagagtag cgcggaaagt cgtaacgggg
    16441 cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct ggatcatctt ccggcacagc
    16501 tttccggcgg tgaacagcaa cgagtggcgc tggcacgagc ctttaatggt cgacctgatg
    16561 tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca gacgggcgat aaaattgccg
    16621 acctgctgtt ttccctcaac cgtgaacatg gcaccacgtt gattatggtg acccacgacc
    16681 tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag ttgcaggagg
    16741 aagcatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat taattgtctg
    16801 gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca gcgatcgcat
    16861 ggagaagggc ttaagccagc aaagccgtga gtttatggcg ggcgatcggg cgttgcgcag
    16921 ttcacgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc tgaaagtcgg
    16981 caagcagctg actttcgcca caatgacctt tgcaggcgac acaccgcagc tggcgaacgt
    17041 caaagcggtg gatgatatct acccgatgta tggcgatctg caaactaatc cccctggcct
    17101 gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc ttaacctgaa
    17161 aacgggcgac accattgacg tgggcgatgc caccttgcgg attgccggag aagtgattca
    17221 ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga tgaatctggc
    17281 ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc gttataaatt
    17341 cggcggcaac gagaaccagc tcgacggcta tgataaatgg ttgttacctc agcttaaacc
    17401 cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat cgatggaacg
    17461 ctcgcaacag ttcctgctgc tttcggcgct tctgaccttg ctgctggcag tggcagcggt
    17521 ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtggcga tcctcaaaac
    17581 gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt tgatggtgct
    17641 gacgctttca gccgttaccg gtggggccat aggcctgttg ttcgaaaacg tgttgatggt
    17701 gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc cgtggctgtg
    17761 ggcgcttggc accatgacgg tcatctcgct gctggtgggg ctacgaccat accgcttgtt
    17821 gctggcaacg cagcctttac gcgtattacg taatgatgtg gtagcgaacg tctggccgct
    17881 gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg gattaatggg
    17941 tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt tgctgtgcgg
    18001 tgtgctgggc tggatgcagc tgaatgtact tcgccgcatg acgctgaaat cgctacctct
    18061 gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc agctttcggc
    18121 attttcgctc tcctttatgc tgctggcgct gttgctggta ttgcgtggcg atctgctcga
    18181 ccgctggcag cagcagctac cgccagaaag cccgaactac tttttaatta acatcgccac
    18241 agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc cggaatcatt
    18301 ttatccggta gtgcgggcgc ggctgacggc gattaacgat aagccgacag aaggtaatga
    18361 agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc ccgatcataa
    18421 tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg aagaggggct
    18481 ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg atacccagga
    18541 gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc ggcctaattt
    18601 ctattttatt ttcccggaag gggcattaga cgggcaaccg cagagctggc ttaccagttt
    18661 ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc cgaccattag
    18721 cctgttagat attggcgcga ttttaaaaca ggtcggtcag gtgctggagc aggtaagtcg
    18781 ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt tgctggcaca
    18841 ggtgcaggtg ggaatgcgtc agcgtcatca ggagctggtg gtgtggcgca cactcggtgc
    18901 ggggaaaaaa ctgctgcgta ccacgttgtg gtgtgagttc gccatgcttg ggtttgtttc
    18961 tggcctggta gccgcaattg gtgcggaaac ggcgctggca gtgttgcagg cgaaagtgtt
    19021 tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca gcggtgcact
    19081 gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta aggcgctgtt
    19141 caggcagttt gcggggtgat gacaggtgtt agattgcctg atgcgctacg cttatcaggc
    19201 ctacgaagag cgtgcaatat attgattttg caagattgtg taggccggat aaggcgttta
    19261 cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca actgtcatgt
    19321 cggtttttta tatctgtatt ctggtgcaac cattctgttc tcacgacaat cgccgtttca
    19381 gaataccgcc catcactgcg tcttaaccca ttccgccact tccgcccact caccgcgaaa
    19441 gacaactttt tccgcttttt tctcaagctg atagcgatac atcgggtcgt aatattcttt
    19501 aagtaacggc accagccagg ccagatgacc gtcggtgctg tcggtggtga gttgcgttgt
    19561 cagcgccgta tccagctggg cagcgagttc gttatagcgc tgtaacccca gccgacgctt
    19621 aatagccgaa agtccgtgat gcaggtattc gcaatattcc tgccagccct gttcgtcgcc
    19681 gtacgcgtgg gtaaaatcat gatgcatacg caagaaatac tcttcgttca ggcgctcaag
    19741 acggatctca aacggatctt ctaccaccgc aatcgccgcc tgagtcattc gctcgcgcag
    19801 gcattccggc aggtgattcg aaccgatcat ccggctttcg tcttccagca cccacaggcg
    19861 caaatcctga cgggcgtcgg tttttagcat ttcggcagcc agcaggtttt caaaactcgc
    19921 ctggctaagt tgtggttgta aggtgcgacc aaacgccgaa ccgcgatgac gcgctaaccc
    19981 ttccagatca acaccgttcg gctgttgctg cactaacagc gttttaccgc tgccggtaca
    20041 accgccaatc agcactatcg gtttttgtgc cagttcaata gtcgcctgaa tcgcggcttg
    20101 gcgcagtgcc ttataaccgc cttccaccag cggataatca atccccgctg catacaacca
    20161 gctttgcaca atatgtgagc gctgaccgcc acgggcgcag cagagaatac cttgcggatt
    20221 ttgcaggcac gctgcccgcc aggcgtccat gcgctgctga cgaatttcac ccgccaccag
    20281 tttatgcccc agcgccagcg ccgcgtctga gccttgctgt ttataacaag tgccaacggc
    20341 ggcgcgttca tcgttattca ttaacggcag gttgatagcg gcgggcattg cgccttgctc
    20401 aaactcgata ggggcgcgaa catcaataat gggcgtatca gcaatcagca gggcacgata
    20461 gtcctgttcc gtgtgtctct cttgcatagt taaaagtgaa cctcaaatca gcttgcgcgc
    20521 tattttacgc gccaacgcgc aaggaaactt gatttttaac tgcgtgggtt gccgaaaatt
    20581 tctaaaaatc cgctgatttc cggcctgcgc tgggtaaaca aggtcacaat atcttctacc
    20641 gctttgccgc tgccaaattt gcgccatgcc agactcaatg gcgaaggagg gcgcatcgtt
    20701 gggattaccc ggctgaccag ttgttgatta tcgatcattg actggcaaag tgattttggc
    20761 aaaaaaccaa tgccaacacc cgccagatgg gcggcgattt tcgtttccat atcgggaaca
    20821 ataatctctt tttgccccgg caatcgccag gcgacgcgtt tggttaaggt tcgggcgctg
    20881 tcttcaatat tgaccgccgg aaagcgccgc aactgcgctt ctgttagcgg ctcttcaacg
    20941 ttcgccagcg gatgatccgc cgccatgaca aagcgccatt gcaccgatcc taagggatca
    21001 agactaaagg tatttgccag cgcctcagtt cccgtgaccc cgatagccag cgaaaaacct
    21061 tcgtacaata gcgagtccca gacgcccata tagatttgtc gggagatgtg aaactgggta
    21121 aaggggtaac gctcattcag ccacgccagc aactgggcga cggcctgggg gttatagagc
    21181 aggttgttga tgacaatatt cacctagcgt tccacgccat cattcacctg ttgcagctcg
    21241 ctgggcatac tttccagcca gctcagccag tctctggcct gggaaagtag atgctcgcca
    21301 gccgctgtca acgtcacgct gcgagtcgtg cggaaaaaca gcgccactcc ggtattctct
    21361 tccagaagtt taatgcgata actgatcgtc gccgtggttt tacataatcg ttctgccgct
    21421 tttgaaaaac ttcctgtttc agcaaccgca atgaaagtcc gcaaggtttc tggatcgaac
    21481 atcttcaggt atcccctttt aaatccgcaa gttgcgtgat tttcttatcc tctgatttat
    21541 cagtattttt acatgataac cctgttcaat ttgtggacta aatctagttt tggaaaaata
    21601 ttccaacttt tgtattgatg ttgttctctt aaggttttag at
//
LOCUS       DAZPAC010000053        21367 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZPAC010000053 DAZPAC010000000
VERSION     DAZPAC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.053"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(37..945)
                     /gene="galU"
                     /locus_tag="ACHUPM_001707"
     CDS             complement(37..945)
                     /gene="galU"
                     /locus_tag="ACHUPM_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR0869509.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(1147..2160)
                     /gene="rssB"
                     /locus_tag="ACHUPM_001708"
     CDS             complement(1147..2160)
                     /gene="rssB"
                     /locus_tag="ACHUPM_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR0869510.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2252..3157)
                     /gene="rssA"
                     /locus_tag="ACHUPM_001709"
     CDS             complement(2252..3157)
                     /gene="rssA"
                     /locus_tag="ACHUPM_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR0869511.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            3270..3728
                     /locus_tag="ACHUPM_001710"
     CDS             3270..3728
                     /locus_tag="ACHUPM_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR0869512.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3778..4620
                     /gene="purU"
                     /locus_tag="ACHUPM_001711"
     CDS             3778..4620
                     /gene="purU"
                     /locus_tag="ACHUPM_001711"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR0869513.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            4780..4864
                     /locus_tag="ACHUPM_001712"
     tRNA            4780..4864
                     /locus_tag="ACHUPM_001712"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4814..4816,aa:Tyr,seq:gta)
     gene            4912..5043
                     /locus_tag="ACHUPM_001713"
     ncRNA           4912..5043
                     /ncRNA_class="other"
                     /locus_tag="ACHUPM_001713"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5227..5904)
                     /gene="narI"
                     /locus_tag="ACHUPM_001714"
     CDS             complement(5227..5904)
                     /gene="narI"
                     /locus_tag="ACHUPM_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR0869514.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(5904..6614)
                     /gene="narJ"
                     /locus_tag="ACHUPM_001715"
     CDS             complement(5904..6614)
                     /gene="narJ"
                     /locus_tag="ACHUPM_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR0869515.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(6611..8149)
                     /gene="narH"
                     /locus_tag="ACHUPM_001716"
     CDS             complement(6611..8149)
                     /gene="narH"
                     /locus_tag="ACHUPM_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0869516.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(8146..11889)
                     /locus_tag="ACHUPM_001717"
     CDS             complement(8146..11889)
                     /locus_tag="ACHUPM_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR0869517.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(12282..13673)
                     /gene="narK"
                     /locus_tag="ACHUPM_001718"
     CDS             complement(12282..13673)
                     /gene="narK"
                     /locus_tag="ACHUPM_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR0869518.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            14012..15808
                     /gene="narX"
                     /locus_tag="ACHUPM_001719"
     CDS             14012..15808
                     /gene="narX"
                     /locus_tag="ACHUPM_001719"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR0869519.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            15801..16451
                     /gene="narL"
                     /locus_tag="ACHUPM_001720"
     CDS             15801..16451
                     /gene="narL"
                     /locus_tag="ACHUPM_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR0869520.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(16452..17846)
                     /gene="ychO"
                     /locus_tag="ACHUPM_001721"
                     /gene_synonym="iatA"
     CDS             complement(16452..17846)
                     /gene="ychO"
                     /locus_tag="ACHUPM_001721"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR0869521.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            18031..18384
                     /gene="ychN"
                     /locus_tag="ACHUPM_001722"
     CDS             18031..18384
                     /gene="ychN"
                     /locus_tag="ACHUPM_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR0869522.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(18428..19123)
                     /gene="chaC"
                     /locus_tag="ACHUPM_001723"
     CDS             complement(18428..19123)
                     /gene="chaC"
                     /locus_tag="ACHUPM_001723"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR0869523.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(19281..19511)
                     /gene="chaB"
                     /locus_tag="ACHUPM_001724"
     CDS             complement(19281..19511)
                     /gene="chaB"
                     /locus_tag="ACHUPM_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR0869524.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            19781..20881
                     /gene="chaA"
                     /locus_tag="ACHUPM_001725"
     CDS             19781..20881
                     /gene="chaA"
                     /locus_tag="ACHUPM_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR0869525.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            21284..>21367
                     /locus_tag="ACHUPM_001726"
     CDS             21284..>21367
                     /locus_tag="ACHUPM_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0869526.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIV"
BASE COUNT         5248 a         5670 c         5219 g         5230 t
ORIGIN      
        1 ctcaacgccg tttcgtggat aacaccgata cgaatgttac ttcttaatgc ccatctcttc
       61 ttcaagccag gctttaaatt ccgtgccaag ggtgttatga cgaataccgt attcaacgaa
      121 ggcctgcatg taacctaatt tattaccgca gtcatggctc ttccctttca tatgataggc
      181 ttccaccgtt tctttttcga tcagcatatc aattgcgtcg gtgagctgaa tttcatcacc
      241 agctcccgga ggggtttttg ccagcaacgg ccaaatatcc gcgctaagta cgtaacgacc
      301 cacaatagcg agattagacg gcgcaacatc cgctttcggt ttttctacca caccaaccat
      361 cggtacgctt tcacccggcg ctaattcaac gcctttgcaa tccacaacgc catatgcggt
      421 cacatcagca accggttcaa ccatgatctg gctatgaccc gtttcatcaa agcggcggat
      481 catctctgcc aggttatcct gtgacaaatc ggattcatat tcatccagaa taacatcagg
      541 caaaataaca gctaccggtt catcacccac taccgggtga gcacacaata ccgcgtgtcc
      601 caggcctttc gccagaccct gacgaacttg cataatagtc acgtgcggtg gacaaataga
      661 ctgcacttca tcaagcagtt gacgttttac acgtttttcc agcattgctt ccagttcaaa
      721 actggtatca aagtggtttt caatagagtt tttagatgag tgtgtaacca gcacaatttc
      781 agtaatgcca gccgcaatac attcattcac gacgtattga attaatggct tatcgacaag
      841 tggcagcatc tctttcggga tggctttcgt cgccggcaac atcctggttc ctaatcccgc
      901 aacggggata acggcttttt tgactttcgt attaatggca gccatttaaa ttctcctgga
      961 ctgttcgtgt tttgaacgtg ttcatatttc tgtatcgcat cccagtatat cagcaccccg
     1021 aaggggctaa gttctgaaaa ggaggcgagt tagcataatt aagacaatta cttatacgaa
     1081 tggcgacgat agtaccacca gccgacatta gcaggtaatg caaatttagc ccgcgttatc
     1141 gtttgctcat tctgcagaca acatcaagcg cagtcgacca ccggttcccc atatttggca
     1201 ttgccaggca tcgcatcgct ggctcaattg attcaaataa gcgttaccta aagtgcctaa
     1261 cggaacacca ttactgattt gcacctggtg ttcgccggta tttaacgtcg cattcagacc
     1321 cgcagaaacc agaatgagat ttttcagttc gcgatgataa tagccaacta atagcggaaa
     1381 ctgccccggc agattggcct gacggagtaa atggtttacc tgcttcaata acgcgcccaa
     1441 ctctggcaac cgttgatttt ggtgtgcaag ctgttcctgt aataatccgt taaacaatgc
     1501 gcgtaataac aaggcagcaa gtacgccatt atgtccagct cgggtgacat caaggcaata
     1561 aaatgccaga tcgttttccg aaagtgcggc aatatcaagc accaggccgg gtttgtccgc
     1621 ggcaaccaat tgacgataat taacccggca atgggaaatc acctgctgaa ccggcggttg
     1681 tagttcctgt aataatttcg ccgctgcggc agggttatca accattgcat cccagtcgcg
     1741 aaaaagcctt tcctcttcct caacgcgcga attaaacatg ctgggataga gacaggcaaa
     1801 aaccatctcg cgcaagcgat tcagatcttt aactggtttc agcaaaacat cttcaacgcc
     1861 cagacgtaac gctttggcaa tatctgccat attttcagtg gcagatatca ccagaactgg
     1921 ggtctggtcg cctctgttac gtatatgctc cagcagttta agcccgttca ttcgtggcat
     1981 cgcgatatca catatcatca ggtctggagt gaaacctccc agcaactcaa gggcatccac
     2041 cccatcagcc gccagtaccg ttgtcgctcc caatgaggaa aaccatgaat ccagaagcga
     2101 gcgaaatacc tgctcatctt caacaatgag aatctgtttt ccgaccaatg gctgcgtcat
     2161 gttctctccc ctgactggct ttactcaata gtggcatgct attgccacct gcgcctgtca
     2221 gaattaactt aagtgtagat aaaaattctg gtcaaatgtt ggtgcgtacc aacggcaaaa
     2281 gttcgtccat tttcctttcc actgccagct gtccggccgc aatggcagcg tgcgcgcgat
     2341 ggaaatcaag cgtagatatt tgcgggcaaa caggttgaat cagaatatcg ggcggatcac
     2401 ctgccatgcg gttcctttta aggcggttct ccaacacctg gatagacgtg gtcataatct
     2461 ctgttgccgt tggcgctgtc acagcccgac gtgtcgttat gctgcctaac ctttctttca
     2521 gacgcgcatg ccacggtaga gaatcaccat tctcgctatt ttcttcactg acattaaagg
     2581 agagcagatc ttgttgcatc aaatgagcat cgtgctgcag gtcgaccgct atcacaatat
     2641 cagcccccaa tgcacgcgta agagaaatag gaattgggtt aactactgcc ccatcaacca
     2701 gccagtagcc gttatgtgca acgggtgcca tgagtcctgg aatactgcat gatgcgcgaa
     2761 tagcaagatg gagatcgcct tcagtaaacc ataattcacg ccccgtactt aaattggtgg
     2821 caacagccgc aaagcgacgg gaacaatttt cgatctctgt ttccggcatt atttcgcgat
     2881 attgattgaa gacacgctcg ccgcgcagta acccaccgcg ctgccaggag aggtccatca
     2941 ggcgtaaaac gtcccaataa ctgaaagagg ttacccaatc ttccagcgca gataatcgat
     3001 cgcatgcata ggccgcgccc accagcgaac caattgaaca tcctgcaacg atatcaattt
     3061 caatacccac tttttttagc gcattaataa cgccaatatg cgaccaacct ctcgccgcgc
     3121 cagatcccag cgccagccct atttttatct ttctcattat tcccgcaaga ttcccctgga
     3181 atgcaatcgt agccacatcg ctactgctcg gttaacatag tgctactcag gcggttaata
     3241 cgccgttatt gtttccaggg agattatttg tgtctcagct ttgtccctgt ggtagtgctg
     3301 tcgagtatag cctatgttgc cacccttatg tgtctggtga aaaggttgca cctgatccag
     3361 aacatctcat gcgttcgcgt tactgcgctt ttgtgatgca agacgcagat tatttaataa
     3421 agacctggca tccctcttgt ggagcagcgg cgttacgtgc cgaattgatg gccggatttg
     3481 cgcatactga atggttggga ttgaccgtat ttgaacattg ttggcaggac gccgacaata
     3541 tcggatttgt cagttttgtt gctcgattta ccgaaggtgg caaaacaggt gcaataattg
     3601 aacgttcgcg atttttgaaa gaaaacggtc aatggtacta tattgacggt acacgtccgc
     3661 agtttggtcg caacgatccc tgcccttgtg gttcaggtaa aaaatttaaa aagtgctgcg
     3721 gccaataatg gttgacggta cggtttagca aacactctca acaaggtttt tccagcaatg
     3781 cattcactcc aacgtaaagt tctgcgtact atttgtccgg accaaaaagg tctgatcgca
     3841 cgtattacca atatttgcta caagcacgag ttaaatatcg tacagaacaa tgaatttgtt
     3901 gatcaccgta ccgggcgctt ttttatgcgc acggaactgg aagggatttt taatgattcc
     3961 accctgctgg cggatctcga tagcgcattg ccagaaggct ccgtgcgtga gctgaatcct
     4021 gccggtcgtc gccggatagt gattctggtc actaaagaag cgcattgcct tggcgatttg
     4081 ttgatgaaag ccaattacgg cggcctggat gtcgaaatcg cggcagttat tggtaaccac
     4141 gatactttac gttctctggt tgagcgtttt gatattccgt ttgagctggt aagccatgaa
     4201 gggttaagcc gcaacgagca cgatcaaaag atggcggaag ccattgatgc ttatcaacct
     4261 gactatgtgg tgctggcgaa gtatatgcgg gtattaacgc cggaatttgt ggcacgcttc
     4321 ccgaataaaa tcatcaatat tcaccattca ttcctgcctg cgtttatcgg cgcacgtcct
     4381 tatcaccagg cctatgaacg tggtgtgaag attattggcg caaccgctca ctatgtgaat
     4441 gacaatctgg acgaaggccc aatcatcatg caggacgtta ttcatgtcga tcatacctac
     4501 acagctgaag atatgatgcg cgcaggtcgt gacgtcgaga aaaacgtctt aagtcgtgcg
     4561 ctatacaaag tactggcaca gcgcgtcttt gtttacggta atcggacgat tattctttaa
     4621 tcgccagcaa aaataactgg ttacctttaa tccgttacgg atgaaaatta cgcaaccagt
     4681 tcatttttct caacgtaaca ctttacagcg gcgcgtcatt tgatatgatg cgccccgctt
     4741 cccgataagg gagcaggcca gtaaaaagca ttaccccgtg gtggggttcc cgagcggcca
     4801 aagggagcag actgtaaatc tgccgtcatc gacttcgaag gttcgaatcc ttcccccacc
     4861 accatcactt ttcaaaagtc cgtaaactct caaacgaatc cgcaattaaa tattctgccc
     4921 aagcggggaa ggatgagaag cttcgaccaa ggttcgactt gagcgccagc gagagagcgt
     4981 tgccgcaggc aacgacccga agggcgaagc gcgcagcgct gagtaatcct tcccccacca
     5041 ccatcgcttc cttaaataat caaaacctgt tactccgagc taattttgcc cgatggcacc
     5101 acgtttaccg ggcctacaat atcctcccct gtaggtcgga taagcgaagc gcatccgaca
     5161 actcaccccg caccgtgccc ataaaaaaac cccgccgaag cagggtctat gtgaactaaa
     5221 attcgcttag tgacgagcgc gcaccagctg gtacttacgt gtcagatact ccaccggtac
     5281 gctccagatg tgtaccagac gcgagaacgg gaacagcagg aataacgtca tccccagcac
     5341 caggtgcaga cggaagataa acgccacacc atcgaggtgt tgagaagcgc caccgtggaa
     5401 ggtcaccacc gactgcgccc agccaaccag tttcatcatc tcgctaccgt ccatatgctg
     5461 agcggagaac ggaatggtca acaggcccag cgcgcactgg ataacgagca gcgacaggat
     5521 caggatatcc gctccggtag tggttgcacg cacgcgtggg ctgaacagac gacgcttcag
     5581 caacagcacg ccgccaatca gacacagcac gccgctggca ccaccagcaa acatcgccat
     5641 tttctgtttc acttcaatcg gcagccaggc ttcatacatc cagtgcggcg tcagcatacc
     5701 gaagaagtga ccaacaaaaa tccccagaat accgatatgg aacaggttcg acgccaggtt
     5761 catccctttg cgatccagca tctggctgga cgccgcgcgc caggtgtact gcccgtagtc
     5821 ataacgcagc cagctaccaa tcaggaagac cgccccggct atgtacgggt agatatcaaa
     5881 gaagaacata ttcaggaatt gcattagtgc tgtcctccgg tggtgatatt cagatattgc
     5941 ggcgcgacgg caccggcaaa gcgacgctga tgagcagtga ttgctgaatc accgcagcct
     6001 ttgtcagcaa agaatttaac ctgctcttct tcccagacag catccagcgc ctgcggcgta
     6061 tcatcgcgcg cttcgtcggc aattttttcc gccactttgt cgctgtcgat agcggtattg
     6121 gccaatttca gcagcagatc aaacatcacg gcataacggc tttcacgctg ttgcagacgc
     6181 gcgctcagca atgccagaat cggcgcgata tctttcaaac cttccacggc ttcgctttgc
     6241 ggcagctgcg acaggtactc cagatacagc ggcagatggt ccggtaattc gcggctgttt
     6301 aactgcaagc cgtgctgctc gtactgcgcc agcaggtcca ccatcgcctg gccgcggtca
     6361 cgggattcac cgtgaacatg ttcaaatagc aacagtgacg tggcgcgacc acggtcgaac
     6421 agttcgctgt actgggcctg ggcatcgagc ggatccatcg ccgttaaatc gcgcaggaaa
     6481 atgcccagcg catgggcatc ttctttcgac agatttttcg acgcagcaat cgcctcaaac
     6541 atctcttgtt gatgctgcca taaggcagca tccggatatt caaggagacg cgatacaatc
     6601 acgagttcga tcatggatgc ggctccgttt tgctggtcac atcgatggca tcgatacgac
     6661 ggctgttgaa cagattgaat ttggtatctg aaccgtggca gccatcacca aaggtaaagc
     6721 cgcagccatt tttctccggg aaggcttccc gtgccagttc acgatgacta ctcggcacca
     6781 caaagcgatc ttcgtagtta gcaatcgcca gataacggta catctcctgt gcctgggctt
     6841 cggtcagacc gacctcttcc agcgcacggg tatcaacttt accgtcaacg gtttcagcac
     6901 gtttgtaatg acgcatcgcc agcatacgtt tcagtgcgcg cagtaccggt ttggtatcac
     6961 cggcggtcag cagattcgcc agatactgta ccggaatacg caagctttcg acgtctggca
     7021 gaatgccgtt gctacccagc tcacccgcgt ctgctgcaga ctgaatcgga gacagaggcg
     7081 gcacgtacca gaccatcggc agtgtgcgat attctggatg cagcggcagc gccagtttcc
     7141 attccattgc cattttataa accggcgact gctgtgcggc ttcaataacg ctcagcggaa
     7201 taccgtcttt aatcgcctgc tcgatgactt tcggatcgtt cggatcgagg aacacctcca
     7261 gctgacgctg gtaaagatct ttctcgttct cggtgctggc tgcacgttca atcgcgtcgg
     7321 catcgtacag cagcacgcca agataacgga tacgaccgac acaggtttct gagcacacgg
     7381 tcggctgacc cgcttcaata cgcggatagc agaagatgca cttctcagac ttaccgctct
     7441 tccagttgaa gtagattttt ttgtacgggc atccggtgat gcacatacgc cagccacggc
     7501 atttatcctg gtcgatcagg acgatgccat cttcttcacg cttgtaaatc gcaccgctcg
     7561 ggcaggtcgc cacacatgcc gggttcaggc agtgttcgca caggcgcggc aaatacatca
     7621 taaaggtgtt ttcgaactgg ctatacatcg ccttctggat gttgtcgaag ttcttgtctt
     7681 tcgccagttt gtcaaactca ccacccaggt catcttccca gttcggccct ttttcgattt
     7741 tcgccatccg ttccccggta atcagcgaac gcggacgggc aatcggctgc gatttgctgc
     7801 cttccggcgc ggtatgcagg ttctgatagt caaaatcgaa cggctcgtaa taatcgtcga
     7861 tccccggcag atgcgggtta gcgaagattt tacccagcaa catggcacgg ttacccatgc
     7921 gcggctgcag tttgccgttg attttacgga tccagccgcc tttgtatttt tcctggtttt
     7981 cccagtcagt cgggaagccc tgacccggct tggtttccac gttgttgaac cacgcgtatt
     8041 ccacgccttc acggctggtc cagacgtttt tacaggtaac tgaacaggtg tggcagccga
     8101 tgcacttatc gagattcagc accatgccga cttgtgaacg aattttcatt ttacgctctc
     8161 ctgtacctgg tcattgcctt cgccatctaa ccagtcaatg ttcttcatct tacgcactac
     8221 aacaaactca tcgcggttag aacctacggt gccgtagtag ttaaagccgt atgccagatg
     8281 ggcatagccg ccgatcatat gcgtcggttt cggcgtgata cgggtgaccg agttatggat
     8341 accaccacgc tgttgggtaa tttccgaacc aggcaggtta acgatacgtt cctgcgcgtg
     8401 gtacatcatg gtcatccctg ccggaacacg ctggctgaca accgcacggg cagtcagagc
     8461 accgttgctg ttgaatactt caatccagtc gttatcggcg atacccagat ctttggcatc
     8521 ggcttcactc aaccagacca ccggaccacc gcgacctaaa gtcagcatca gcaggttgtc
     8581 gctgtaggtg gagtggatac cccacttctg gtgcggcgtc aggaagttga gcgctttttc
     8641 cgggttgccg ttggatttct ggcctatcac ttctttcacc gaacgggtgt cgatcggcgg
     8701 acgataaacc agcaggcttt caccgaaatc acgcatccac tggtgatcct gatacagttg
     8761 ctgacgacca gagagcgtac gccatgggat cagctcgtga acgttggtgt aaccggcgtt
     8821 gtaagaaacg tgttcatctt ccagaccaga ccaggtcggg ctggagataa ttttgcgcgg
     8881 ctgtgcctga atatcgagga agcggatctt ctcgtcttct ttattcagcg ccagatgcgt
     8941 atggtcacga ccggtaaatt cgctcagggc agcccaggct ttcacggcta cctgaccgtt
     9001 ggtttccggt gccagtgtca ggatcatctc tgccgcatca attgcggtgt tcagcatcgg
     9061 ctggcctttc gccggacctt ccgctttggt gtagttgagc ttacgcagca gatccatctc
     9121 gctctgggtg ttccaggcaa tccctttacc gccattaccg attttctcca tcaacgggcc
     9181 aatagaggta aagcgttcgt aagtcgccgg ataatcgcgc tctacgacca taatgtgtgg
     9241 cgcggtttta cctgggatca ggtcgcactc gccttttttc cagtctttca catccagcgg
     9301 ctgcgccagt tcagcggcag agtcatgctg gataggcagc gtgacgatgt cggtttcttt
     9361 acccagatgg ccgacgcaca cttcggagaa tttcttcgcg atggctttgt agatttccca
     9421 gtcgcttttc gcttcccagg ccggatcgac cgccgcagac agcgggtgaa taaacggatg
     9481 catatccgaa gtattcatgt cgtctttttc gtaccaagtc gccgtcggca aaatgatgtc
     9541 ggaatagaga caggtgctcg acagacggaa gtccagcgta accaccagat ccagcttgcc
     9601 ttccagacca ttgtcctgcc agtccacttc ttccggcttc acgccgccct gttgccccag
     9661 atctttaccc tggataccgt gctccgtccc cagcaggtac ttgagcataa actcatgacc
     9721 tttaccggaa gaaccgagca ggttagaacg ccagatgaac aggttgcgcg ggtggttttt
     9781 accgttttct ggttgttctg ccgcaaaacg gatggaaccc tctttcaggg atttcaccgt
     9841 atagtccacc ggattcatcc cggctttttc cgcttcgcca gcgatagtca gcgggttagt
     9901 gcctaactgc ggtgcagacg gcagccagcc catgcgttcc gcacggacgt taaagtcgat
     9961 caagtgtccg gtatagcggg atttgtccgc catcggtgac agcaactctt ccgccgtgac
    10021 ggtttcataa cgccactggc tggagtggtt atagaaataa gaagtgctgt tcatgtgacg
    10081 cgccggacgc tgccagtcaa gggcaaacgc cagcggctgc cagccggttt gcggacgcag
    10141 tttttcctga cctacatagt gcgcccagcc gcccccgctc tgaccgacac agccgcagaa
    10201 aatcagcatg ttgatcagac cacgatagtt catatcgagg tgataccagt ggttcagccc
    10261 cgcaccgacg ataatcatcg aacgaccgtg cgttttatca gcgttatcgg caaattcacg
    10321 ggcgatgcga ataatctggc tgcgagaaac gccggtaatc tgctcggccc aggccggggt
    10381 ataagctttc acatcgtcat agctggttgc acagttaacg tcgttcaggc cacgttccag
    10441 accgtagttt gccagcgtca gatcataaac ggtgctcacc agggcggtgc tgccatcagc
    10501 cagttgcagg cgtttcaccg gcagtttgtg cagcagcacg ttttccagtt ccactttgtt
    10561 gaagtgttcc gtgccgtcgc caccaaagta cgggaagcct acctcagcga tctcatcctg
    10621 gctacccagc aggctcagtt gcagctcggt ttcttcgcca gttttgccgt cgcgctgctc
    10681 aagattccat ttgcccttct cgccccagcg gaagccaata gaaccgttcg gcgcaaccat
    10741 ttcgccattg gtattaaagg cgacagtttt ccattccgga ttgttttcct ggcccagcgc
    10801 atcaaccaga tcagcagcgc gcagcatacg acctgcagcg tagtaaccgt cgcgttcttc
    10861 cagcatcacc agcatcggca tgtcggtgta gcgacgcaca tagtcggtga aatactggct
    10921 tgggttgtcg aggtggaatt cacgcagcat tacgtggccc atcgccagcg ccattgccgc
    10981 atcggtgccc tgtttcggtg ccagccacag atcgcacagt ttggcgattt cagcgtagtc
    11041 tggtgtgacg gcaacagttt tggtgccttt gtaacgcact tcagtaaaga agtgagcatc
    11101 cggggtacgc gtctgcggca cgtttgaacc ccaggcgatg atgtagctgg agttatacca
    11161 gtcagcagat tccggtacgt cagtttgctc gccccaggtt tgcggagacg caggaggcag
    11221 gtcgcagtac cagtcgtaga agcttaagca agtaccgcca atcagcgaga gatagcgtgc
    11281 acccgatgcg taagaaacca tcgacattgc cggaattggc gagaaaccag caacacggtc
    11341 cgggccgtag tttttgatgg tgtaaacgtt agatgcggcg atcagttcgt tcacctcctg
    11401 ccaggaagaa cgaacaaatc caccgcgtcc acgcgcctgc ttaaagcttt tcgctttatc
    11461 ggcgtcttca atgatagaag cccatgcctc aaccggatcg ctatgcagcg ccttcgcttc
    11521 acgccacatt ttcatcaggc gtttgcgcat catcgggtat ttcaggcggt tggcactgta
    11581 aagataccag gagtagctgg caccgcgcgg gcagccgcga ggttcatggt ttggcagatc
    11641 cggacgggta cgcggatagt cagtctgctg ggtttcccag gtgaccagac cgtttttgac
    11701 gtagattttc cagctgcagg agccggtgca gtttaccccg tgggtagagc ggacgatttt
    11761 gtcatgctgc caacgctggc gatatccatc ctcccagtca cggttggtat tgagaagctg
    11821 gccatgccca tcggcaaagg tttcaccctt ctgtttgaag tagcgaaacc ggtccaggaa
    11881 tttactcatc ggttttctcc tgtgggagcc tgtcggccct ctgataaatc gacattgcta
    11941 atgtttaagg gcaaggtaac gctctgaaac agcgtgggaa ttgataacga tcaagagtga
    12001 tggggatgaa aaataaagta aattcctaag ataccccttt aggataatta acagccacca
    12061 cataacagac tgtaaattaa gacgttttat acctgtagtt atttaaacta ttaagatttt
    12121 tatgcagatg ggtattaagg agtattcccc atgggtaaca tattaatggc tcttaatgaa
    12181 atccggcgaa ataagccata taatatgagt atgttgtaac taaatgacgc ataaaaaaag
    12241 cgcgacatca tgccgcgcaa aggataatca aatattaaga tttatttttt agaatgccga
    12301 ccatataccg cccaggtaat cactacgcag gcgatataga aaatcaaaaa taccttcatt
    12361 gcgccgactg gcgaacccgt taatgccagc gagctaccaa acgctttcgg gataaagaag
    12421 ccaccaatag cgccaatcgc agagatgaaa cccagcgccg ctgccgtgtc ggttgccgct
    12481 tcacgcatcg cacgttcgtc agaaccccct tctgctttca cgcgatccat tgtcagttta
    12541 cggaagatca ctgaaatcat ctggaaagtg gaaccactac ccagcccagc tgtcaggaac
    12601 agcgccagga agaccgcgaa gaacgccatg aagcttccgc cctgcccgtc agtcggtaag
    12661 gtcaggaaca gcaggccgct gaaaatcgcc atcagaataa agttcaccag cgtgacacga
    12721 gttccgccca gacggtcaga taatgcacca cctgcagaac gcgccagcgc accaataaac
    12781 ggcccgaaga aagcgtattg cagaatctga acatccggga actgcgtttt tgacagcatc
    12841 gcaaagcccg cggagaagcc gataaaggag ccgaaggttg ccagatacag caggctcata
    12901 atccacagat gaccccgttt gagtaccggc aactgctcct tgatggaggc tttcgaggta
    12961 gcaagatcgt tcatgccaaa ccacgccgca atggtgaaga tggcaaggaa cggcacccat
    13021 atccaggacg cattcgccag atacagctca gtcccatccg gctgtttgac gccctggcta
    13081 ccaaatactg cgaaaatcga cagtgatacc accagcggag caaccaactg catgacgctg
    13141 acgcccatgt ttcccagacc accattcaga cccagcgcgc caccctgctt ctgtttcgga
    13201 aagaagaagc tgatgtttgc catactggat gcgaagttcg cgccagcaaa gccgcatagc
    13261 agagagatga tgatgaagac gctataaggc gtggaggtat cctgcacggc aaaacccagc
    13321 cagacgcaag gaataatcag aataccggtg ctgaacgccg tccagcgacg accaccgaag
    13381 ataggaacca taaaggagta tggaacacgt aataacgcgc cagaaaccga aggcagcgca
    13441 gtcaacataa atagctgatc ggtcgtaaaa ttaaaaccga ctttcggtaa gttcaccgca
    13501 acagcgctga acaacatcca tacgcaaaac gccagcagca gacagggaac ggaaatccac
    13561 aggttacggc tggcaatacg ttgaccgcgt tgttgccaga acgcaggatc ttccggtcgc
    13621 caatctgtaa tgacagctcc agtagccctt tcgggggcgg atgagtgact catagacacc
    13681 tctgatactc gtttcgatgt ctgccacctt agtgtctgta gctaaaggca atttgatgta
    13741 aatcaaacga taagataact ttatctatca ttgatattta tcattaccca tagtgagtac
    13801 agtgatttca taaaaattat gagatttttc acggtgctgt aaaatcccta cccttaccga
    13861 tgtaaagcga ctaaccacac ggcaaataag gagtaactct ttccgggtat gggtatactt
    13921 cagccaatag ccgagaatac tgccattcca gaatgtatcg tcacattcat taaggttatt
    13981 gctcatttaa agcctgaagg aagaggtttg catgcttaaa cgttgtctct ctccgctcac
    14041 cctggttaat caggttgcgc ttattgtgtt gctttctact gctattggac tggcagggat
    14101 ggcggtttct ggctggctgg tgcaaggcgt tcagggcagc gcccatgcga tcaacaaagc
    14161 gggatcgctg cgcatgcaaa gttaccgtct gttggcggca gtgccattaa gtgagaaaga
    14221 caagccctta attaaagaga tggaacaaac ggcatttagc gccgagttaa ctcgagcagc
    14281 agaacgagac gaacaactgg cgcaattaca gggtttacaa gattactggc gtaatgaact
    14341 gatccctgca ctgatgcgtg cacaaaaccg cgaaacggtg tcagcggatg tcagccagtt
    14401 tgttgccggg cttgatcagc tggtatctgg ttttgaccgc accacggaaa tgcgcattga
    14461 gacagtggta ctggtccatc gggtaatggc ggtatttatg gcacttttac tggtgttcac
    14521 tattatctgg ttgcgggcgc gactgctaca accgtggcgg caactgctgg caatggcgag
    14581 tgccgtcagt catcgcgatt ttacccaacg cgcgaacatc agcgggcgca acgaaatggc
    14641 gatgcttgga actgcattga acaatatgtc tgcagaactg gccgaaagtt atgccgtact
    14701 tgagcagcgg gttcaggaga aaaccgccgg gctggagcat aaaaatcaga tcctctcttt
    14761 tttatggcag gctaaccgcc gtttgcattc ccgcgccccg ctgtgtgaac gcctgtcacc
    14821 tgtactcaac ggcttacaga atttaaccct gctacgtgat atcgaattgc gggtgtatga
    14881 cactgatgat gaagagaatc atcaggagtt tacctgccag ccagatatga cttgtgatga
    14941 taaaggctgc cagctctgcc cgcgcggcgt attacccgtt ggtgatcgcg gcacgaccct
    15001 gaagtggcgg ctggctgact ctcatacgca gtacggtatt ttgctggcga ccctgccgca
    15061 ggggcgtcat cttagccatg atcaacaaca actgctggat accctggttg aacaactcac
    15121 cgccacgctg gcgctggatc gccatcagga acgtcagcaa cagttgatcg tgatggaaga
    15181 gcgtgccacc attgcgcgcg aactgcatga ttctattgcc caatctctct cttgcatgaa
    15241 gatgcaggtg agttgtttac agatgcaggg cgatgcgctg ccagaaagca gccgcgaact
    15301 gttaagtcag atccgtaacg aactgaatgc atcctgggcg cagttgcgtg aattgctcac
    15361 cacattccgc ttgcagctca ccgagcctgg attacgtccg gcgctggagg cgagttgcga
    15421 agagtacagc gccaaatttg gcttcccggt gaagctggat tatcaattgc cgcctcgcct
    15481 ggtgccttcg catcaggcaa tccacttgtt gcaaattgcc cgtgaggcat taagtaacgc
    15541 cctcaaacat tcgcaagcga gtgaagtcgt ggtgacggtg gcgcaaaacg ataatcaggt
    15601 caaactgacc gtccaggata acggctgcgg cgtgcctgaa aatgccatcc gcagcaatca
    15661 ctacggcatg ataataatgc gcgaccgtgc gcaaagttta cgaggcgatt gccgcgtccg
    15721 ccgtcgtgaa tcaggtggca ccgaagtggt ggtcaccttt attcccgaaa aaactttcac
    15781 agacgtccaa ggagataccc atgagtaatc aggaaccggc tactatcctg ctgattgacg
    15841 atcacccgat gctgcgaact ggcgtaaaac agcttatcag tatggcacca gatatcaccg
    15901 tggttggcga agcgagtaat ggcgaacagg gtattgaact ggcggaatct cttgatcccg
    15961 atctgatcct gttagatctc aatatgcccg gcatgaacgg tctggaaacg ctggataaac
    16021 tgcgcgaaaa gtccctctca gggcgcattg tggtattcag cgtctctaac catgaagaag
    16081 atgtggtcac cgcactgaaa cgcggcgcgg atggttatct gttaaaagat atggaaccgg
    16141 aagatctgct gaaagcattg catcaggcag ctgctggcga aatggtatta agcgaagcat
    16201 taacgcctgt tctggccgcc agcttgcgcg ctaaccgtgc cactactgag cgcgatgtta
    16261 accagttaac cccacgcgag cgcgatattc tcaagctgat tgcccagggt ttgccgaaca
    16321 agatgattgc ccgccgcctg gatatcaccg aaagcacagt aaaagtgcac gtcaagcaca
    16381 tgctgaagaa aatgaagctc aagtctcgcg tggaagcagc ggtatgggtg catcaggagc
    16441 gcattttctg attacggttc ccagcgcagt tcgtcgtttg acaatgcatc gaacggttcg
    16501 acaagcgtta gcgtgatctc attggaggag acacgctgcc cctggttatc ttccaccacc
    16561 accgacaatc gccagtgatt gctcgcccct tccccgttct gccagtcagg catgatcagc
    16621 gtccagccct ccgcgctgtt ggcttgtgcg cccggcgtca aacttaatat ctgcgtatcg
    16681 ccctgccaaa tcagttgccg aataccgtaa cgactgcgga tttgtaattt cagcggcact
    16741 gtttcgccag gttttagatc ccacggcggt gtcgccagaa acaccgttaa cgtttttcgc
    16801 tgtcggtact caagagtcgg tagattattt cgctgcggat tatcatagcg actaccacgt
    16861 aacgactgac tttcggcaac ctcgcccgca gaaagttgtt ttttgagcgg cacaccaaag
    16921 cggtaattaa gattcagtcc gaggttattt tgattttcgc cactttcacc ctgtttatgc
    16981 tgggccgtca cagtgactaa tggcacaggg gtgtaattta atcccagact caacgcgacg
    17041 ggattgtgat aacccgtacc agagttaaac aaatcaacac gctcaccaaa atactgttct
    17101 accctgacac tggtattgag gtgttgatag aacggcatgc gcatccgggc tgtcaggtca
    17161 tacccgcgcg ccatccgttg ttcctgcgtg gctgtctgtt catgccatgc agcaaacggc
    17221 tgataaaagt ttgccgatag tcgcaaatat tcgccccacg cttcggcacc aaagcccgct
    17281 cgctgaagat tttcgtccag caagttgtca taaaaagtgt tataacccac cagccagttg
    17341 ccgcgcgccc agcgttgccc aacgcccaca ttgctcacca acccatcatc ctgctgagta
    17401 agaccaagct ggctccaggt gagataacga tcattatctt gtaacggcac aaaccagctt
    17461 ccacgactgc cggtgaaatg tccttcgtta tccactttga cgtcaacact ggcatttccc
    17521 cacggtgata gccaggactc tacgtgctga ttaacctgtt gactaagcgc gtcgcggact
    17581 tttcccaatg cgaaagcttt tgcctgctcg ccagtatcca gcccgttatc attcatactg
    17641 gtttcgccga aatctttcac aatttcagca aagtgttttt ccccatcatg gttttcggga
    17701 gccatgccta aatccggcag accatcattg ttattatcaa agggatttgc cgctttttgc
    17761 tcgaaggtag attgtgcgtt agctgtaccg cctgccgcca gcaagagtaa ataaaacggg
    17821 ataatgcgag gaacgaaacg gctcaaaacg tcggtaactt ccggtaataa tgaaaaacca
    17881 tagccttaaa gaataacgat tactggcaac ttttgtcgta ttttctcgcg ctttcaggaa
    17941 aatccttaaa aaggtgaccg attggtcaga cgctgcagaa gcagtaagat agaagatgag
    18001 tgtctaattc cctgaataca ggagtaaata atgcaaaaaa tcgtgatcgt tgccaatggc
    18061 gcaccttacg ggagcgaatc cttgtttaac agcttgcggc tggccattgc gttacgagag
    18121 caggagagca atctggatct gcgtctgttc ctgatgtctg atgcggtcac agccgggttg
    18181 cgcgggcaaa aaccagggga aggctacaac attcagcaaa tgctggagat ccttaccgct
    18241 cagaatgtac cggtgaaatt gtgcaaaacc tgtaccgacg ggcgcgggat tagcacactt
    18301 cctcttattg atggggtgga aatcggtact cttgtggaac tggcgcaatg gacgctgtca
    18361 gccgataaag tgctcacatt ttaatgtttg agggggcgtc tgacgcccca ctatgaggga
    18421 agtttactca ggcgaatccc ggccgtaaca caccatccgg ataattctcc gccagcagtt
    18481 tttttaccga taccagcaaa tcattcagcc catcgtcctg cattcccagt ttgataagct
    18541 cctgttccag tgaaaacagg tattgtgcgt tggttcccag cggaccgctc gccgccgcaa
    18601 tcaacggcgc gatgacctga gcgcgagtgt cagattcata ttctggatgt cgcgggtcca
    18661 taataaacac aatggcgttt actgtgcgtc catcatcaag atcaagctga caccaggttg
    18721 gcagataaca gccggtaatc atctcgcgct tccacaacag ggttagttcc tgctccagcg
    18781 tctcttctgg tagtcgatag gcgacgcctg tggtgcgtcc gccctctttc agtgcaagca
    18841 tccgtcccgg ctggtgcgca gtcccgcgcc cggcggtcag gcgcaggcaa aacgcgcgat
    18901 gccatccaac cagtgtaccg gtgcataatt cggtaaactc cagtgccgga ttccacatca
    18961 acgatccata accgaagatc cacaccggtc cctcattagg tcgacaagcc agcgtcgccg
    19021 ccagcgaagc cgcccgttgt tctgctgacc ataagagtga ttcttcaatg gcaccaaatg
    19081 ccgttttaca atcggcattc atcaagaaat cacgcgttat caccttacac ctccgccact
    19141 gcatacttcc ctgcgacaac tttttggtgt cttccggaca tttttaccgc tcattttgaa
    19201 accactaacg ggacaatatg caatccacgg accacttgca aggcaacacc gcacgaatag
    19261 ctttaactaa gctaccggtt ttacgatttt ttatgccatt tatcatcatc ccctttggca
    19321 tattcatgct tcacagcagc ccaggccact ttatgcgcgg tttcttcgcg actggcgtca
    19381 tcacgccgat cttctttatc tttatattga tcccatgcgc tgttgaacgc ttctttatag
    19441 atatcctggg catgagacgg tagaacgtgc tttacgcttt ccggcagatc gcttttcgtt
    19501 ttatacggca tcgttgactc cttttacaaa gttgaacatt aagtgtggtc aacaatatct
    19561 actcttgcca aaacataaga attttcatgt cagactgttc ttaagaaacg ctaaaacaaa
    19621 cattgtctaa acgtaatact tatttttatg ccccaatgag taaaaaatcg acagcggggt
    19681 aaaaattcgt aaaatgttac cgatatccag acactttctg ttatttttcc tacctatttt
    19741 ttacattttc gataaattca tctgcaaagg gataaccata atgtcaaatg ctcaagaggc
    19801 ggtaaaaacc cgccacaagg agacttcgct tattttcccg gttctggcgc tggtagtgct
    19861 gttcctgtgg ggaagcagcc agacactacc agtggtcatt gccatcaatc ttcttgcgct
    19921 tattggtatt ttaagtagcg cctttagtgt tgtccgtcat gcggacgtat tagcccatcg
    19981 cctgggagaa ccttacggtt cgcttattct tagcctttca gtggttattc ttgaagtcag
    20041 tttgatttca gctttaatgg caaccggcga cgccgcgcca acgctaatgc gtgatacgct
    20101 ctattcaatc attatgattg ttaccggtgg gctggttggc ttttcattat tgttgggcgg
    20161 tcgtaagttt gccacccaat atatgaatct gtttggtatc aagcagtatt taattgcgct
    20221 gttccccctg gcgataatcg tactggtatt tccaatggct ctgcctgcgg cgaatttttc
    20281 aaccggtcag gcgttactgg tagcattaat ttctgcggca atgtatggcg tatttttgct
    20341 gatccagacc aaaacgcatc aaagtttgtt tgtctacgag cacgaagatg acagtgatga
    20401 cgacgacccg caccacggta aaccgtctgc ccatagcagc gtgtggcatg ctatctggtt
    20461 gattatccat ctgattgccg ttattgcagt caccaaaatg aatgccagcc cgctggagac
    20521 attgctcgac agcatgaatg cccctgtcgc ctttactggc ttcctggtgg ctctgttgat
    20581 tctgtcgccg gaaggtttag gtgcgttaaa agcagtgttg aacaaccagg tccagcgcgc
    20641 aatgaatctg ttctttggtt cagtgttagc aaccatttcg cttaccgtac ctgtcgtcac
    20701 gctaattgcc tttatgacgg gtaacgaatt acagtttgca cttggtgcgc cagaaatggt
    20761 ggtgatggtg gcctctttag tgctgtgcca tatctccttc tccaccggac gtactaacgt
    20821 gctcaatggc gcagcgcatc tggcactgtt tgccgcctat ttgatgacga tatttgcctg
    20881 acagttatat ctcctccgat gacggaaatc gggggagata agataaattc ctgcttattg
    20941 tatttcttca tactacctgg acagctcgtt aatcagaatt gcgagcctgt accacaacct
    21001 gcacgaaacc actgcaacag ctgttgaaaa gccggaatgt actctttatt gcaaacattt
    21061 cagggatgaa gtgaagatga attgagacgt agtatccaca ccaagccgga attatccggt
    21121 gaggcgcaat gttgcggggg ctttatccct ggtggcattg gttgctggaa agagaaaacc
    21181 cccgcacgtt gagaggtaaa acctgacaac aaaacggggg ctaatcttga aaccagacac
    21241 ttcaagaata gccgccttgt tgtcattaca acaaggcggc tatatgacgc tcgcgcagtt
    21301 tgccatgact ttctggcacg acctggcagc accgatcctg gcggggatta ttaccgcagc
    21361 gattgtc
//
LOCUS       DAZPAC010000054        21197 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZPAC010000054 DAZPAC010000000
VERSION     DAZPAC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21197
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.054"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(82..2955)
                     /gene="gcvP"
                     /locus_tag="ACHUPM_001727"
     CDS             complement(82..2955)
                     /gene="gcvP"
                     /locus_tag="ACHUPM_001727"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR0869527.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(3074..3463)
                     /gene="gcvH"
                     /locus_tag="ACHUPM_001728"
     CDS             complement(3074..3463)
                     /gene="gcvH"
                     /locus_tag="ACHUPM_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR0869528.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(3487..4581)
                     /gene="gcvT"
                     /locus_tag="ACHUPM_001729"
     CDS             complement(3487..4581)
                     /gene="gcvT"
                     /locus_tag="ACHUPM_001729"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR0869529.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            4871..4954
                     /locus_tag="ACHUPM_001730"
     CDS             4871..4954
                     /locus_tag="ACHUPM_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869530.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(5028..6230)
                     /gene="ubiI"
                     /locus_tag="ACHUPM_001731"
                     /gene_synonym="visC"
     CDS             complement(5028..6230)
                     /gene="ubiI"
                     /locus_tag="ACHUPM_001731"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR0869531.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(6253..7431)
                     /gene="ubiH"
                     /locus_tag="ACHUPM_001732"
                     /gene_synonym="visB"
     CDS             complement(6253..7431)
                     /gene="ubiH"
                     /locus_tag="ACHUPM_001732"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR0869532.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(7428..8753)
                     /gene="pepP"
                     /locus_tag="ACHUPM_001733"
     CDS             complement(7428..8753)
                     /gene="pepP"
                     /locus_tag="ACHUPM_001733"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR0869533.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(8779..9357)
                     /gene="ygfB"
                     /locus_tag="ACHUPM_001734"
     CDS             complement(8779..9357)
                     /gene="ygfB"
                     /locus_tag="ACHUPM_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR0869534.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            9525..9854
                     /gene="zapA"
                     /locus_tag="ACHUPM_001735"
     CDS             9525..9854
                     /gene="zapA"
                     /locus_tag="ACHUPM_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR0869535.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            9896..10079
                     /gene="ssrS"
                     /locus_tag="ACHUPM_001736"
     ncRNA           9896..10079
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHUPM_001736"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            10100..10702
                     /locus_tag="ACHUPM_001737"
     CDS             10100..10702
                     /locus_tag="ACHUPM_001737"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR0869536.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(10803..10862)
                     /locus_tag="ACHUPM_001738"
     CDS             complement(10803..10862)
                     /locus_tag="ACHUPM_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0869537.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(11194..11253)
                     /gene="ibsC"
                     /locus_tag="ACHUPM_001739"
     CDS             complement(11194..11253)
                     /gene="ibsC"
                     /locus_tag="ACHUPM_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR0869538.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(11482..12714)
                     /gene="serA"
                     /locus_tag="ACHUPM_001740"
     CDS             complement(11482..12714)
                     /gene="serA"
                     /locus_tag="ACHUPM_001740"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR0869539.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(12970..13629)
                     /gene="rpiA"
                     /locus_tag="ACHUPM_001741"
     CDS             complement(12970..13629)
                     /gene="rpiA"
                     /locus_tag="ACHUPM_001741"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR0869540.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            14059..14952
                     /gene="argP"
                     /locus_tag="ACHUPM_001742"
     CDS             14059..14952
                     /gene="argP"
                     /locus_tag="ACHUPM_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR0869541.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            15155..17299
                     /gene="scpA"
                     /locus_tag="ACHUPM_001743"
     CDS             15155..17299
                     /gene="scpA"
                     /locus_tag="ACHUPM_001743"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR0869542.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            17292..18287
                     /gene="meaB"
                     /locus_tag="ACHUPM_001744"
     CDS             17292..18287
                     /gene="meaB"
                     /locus_tag="ACHUPM_001744"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR0869543.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            18298..19083
                     /gene="scpB"
                     /locus_tag="ACHUPM_001745"
     CDS             18298..19083
                     /gene="scpB"
                     /locus_tag="ACHUPM_001745"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR0869544.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            19107..20585
                     /gene="scpC"
                     /locus_tag="ACHUPM_001746"
     CDS             19107..20585
                     /gene="scpC"
                     /locus_tag="ACHUPM_001746"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFR0869545.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(20582..>21197)
                     /locus_tag="ACHUPM_001747"
     CDS             complement(20582..>21197)
                     /locus_tag="ACHUPM_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0869546.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         5093 a         5755 c         5407 g         4942 t
ORIGIN      
        1 ggactgtctg agcacttttg tactttgtca tctgactaaa aaggcgccga agcgccttta
       61 gaaaatagtc gaatcagtga attactggta ttcgctaatc ggtacgcagg agcagaacag
      121 gttacggtcg ccgtaaacat catccagacg tttcaccgtc ggccagtatt tgtctgccac
      181 gcctgccggg aataccgcaa cttcacggct gtacggatgc gcccactcgg cgaccagttc
      241 gctctgaatg tgcggcgcgt tcaccagcgg gttatcttcc agcggccaga cagcggcttt
      301 cacctggtca atttctgcgc ggatagccag catcgcgtcg ataaagcgat ccagttccac
      361 tttgctttca gattcagtcg gttcaaccat cagcgtaccc gccaccggga acgacatcgt
      421 cggcgcgtgg aaaccgtagt cgatcaggcg cttggcaata tccagctcgc tgatgccggt
      481 ttcttctttc agcgggcgaa tatcgagaat acattcgtgc gccacgcgac cgtcgcgacc
      541 ggtatacagc accgggaagg catcctgcag gcggctggca atatagttgg cgttgaggat
      601 tgccacctgg cttgcttttt tcagcccttc tgcgcccatc atgcggatgt acatccagct
      661 gattggcagg atagaggcgc taccgaacgg tgccgcagaa actgcgccct gacgggttaa
      721 catgccttcg atttgcacca cgctatgacc cggtacaaac ggtgccagat gcgctttcac
      781 accgatcggt cccatacccg gaccaccacc gccgtgcgga atgcagaaag ttttatgcag
      841 gttgaggtgc gaaacgtccg cgccaataaa gcccggcgag gtgatgccaa cctgggcgtt
      901 catgttcgcg ccatcaaggt aaacctgacc gccgaactga tgcacgactt cacacacttc
      961 acggatcgtt tcttcataca cgccgtgggt agaaggataa gtcaccatga tacaggagag
     1021 gttatcgccc gcctgttccg ctttcgcgcg cagatcagtc agatcgatgt tgccgttttt
     1081 atcacacgcc acaaccacca cctgcattcc tgccatatgt gcagaagcgg ggttagttcc
     1141 gtgcgcagaa gccgggatca ggcagatatc gcgatgccct tcgttgcggc tttcatgata
     1201 atgacgaatc gccagcaggc ccgcgtattc gccctgtgcg ccggagttcg gctgcataca
     1261 aacggcgtcg taaccggtca gtttcaccag ccagtcagcc agctgcgcaa tcatctgctg
     1321 ataaccttca gcctgttccg gcgggcaaaa cgggtgcagt tcggcaaatt ccggccaggt
     1381 gattgggatc atctcggcgg cggcgttcag tttcatggtg caggaaccca gcgggatcat
     1441 cgcctgattc agcgccagat ctttacgctc cagcgagtgc atatagcgca tcatttcggt
     1501 ttcgctgtgg tagcgattaa acaccggatg ggtgaggatt tcgtcgtcgc gcagcatcgc
     1561 aggctggata gagcggctgt cgtgagccac gtctttgtcc agcgtgtcga tctccagccc
     1621 gtggttatcg cccagcagca cgctgaaaag ctgcattacg ttttcacgcg tggtggtttc
     1681 atcaagggtg atccccacgg cgttcaggat atcgctacgc aggttgattt cagccgcttc
     1741 ggcacgcgtc agtacgcccg ctttgtcggc cacttccaca cacaaggtgt cgaaatagtg
     1801 cgcatggcgc agtttcagac ctttttgttg caggcccgcc gccagaatat cggtcagacg
     1861 gtgaatgcgg ttagcgatac gtttcaggcc aaccgggccg tgataaacgg catacaggct
     1921 ggcgatgttt gccagcagta cctgggaagt acaaatgttg gagttcgctt tctcacgacg
     1981 aatatgttgc tcgcgagtct gcatcgccat gcgcagcgcg gtattgccag ctgcatcttt
     2041 cgatacaccg ataatacggc ccggcattga gcgtttgtat tcatctttcg ccgcaaagaa
     2101 tgccgcgtgt gggccaccgt agcccatcgg cacgccgaag cgttgcgccg aaccaaaaac
     2161 aatatccgcg ccctgtttac ccggcgcagt taacagcacc agcgccataa tatcggcggc
     2221 aacgctgacc acaattttgc gtgatttcag ttcgctaata agcgcagtgt agtcgtgaat
     2281 ttcaccggta gtgcctacct gctgtaacag cacgccgaag acgtcctgat ggtcgagcac
     2341 tttttgcgcg tcatcgacaa tcacttcaaa accaaaggtt tcggcacgag tacggaccac
     2401 atccagcgtt tgcggatgca catcggaagc cacgaagaag cggttggcat ttttcagttt
     2461 gctgacgcgt ttcgccatcg ccattgcttc ggcggcagcg gtggcctcgt ccagaagaga
     2521 agcagaggcc atatccagtc cagtcaaatc cagcgttacc tgctggaagt tgagcagtgc
     2581 ttcaaggcgg ccctgggaga cttcaggttg atacggagtg tacgcggtat accagcccgg
     2641 attttccagc atgttacgca ggataaccgg cggtagctgc acggcggtgt aacccatgcc
     2701 gatgtaagac gtgaagcgtt tattgcgact ggcaatagcc ttgagttctg ccagtgcggc
     2761 gtattcggtc gccggtgcgc caacctgcgg cggggtcgca agctgaatat ctttcggtac
     2821 aatctggccg gtcagcgcgt ttaacgattg tgcgccaacg gcattcagca tttcttgctg
     2881 ttgcgcggcg tccggtccga tatggcgttc aataaaagcg ccgctgtttt caagctggct
     2941 taacgtctgt gtcatgagcg atggttcctg aaacgtgcag tgaattgtga acctctctcc
     3001 ttacgaagag agtgagggtg aggcataaat tttcctcacc ctgatcctct cccgcagaag
     3061 aggaataaag ccgttactcg tcttctaaca atgcttcgta tgcggtcgca tccagcagtg
     3121 attccagttc gctttcatcg ctggctttga ttttaaagat ccagccacct gcatacggtt
     3181 cgctgttcac cagttccggg gaatcgctca gtgcgtcgtt taccgccacg atttcaccgc
     3241 ttactggcgc ataaatgtct gacgccgctt ttactgattc ggcaaccgcg cagtcatcgc
     3301 ccgcgctaac cgttgcgccc acttccggca ggtcaacaaa caccatatcg cctaacagct
     3361 cctgagcatg ttcggtaata ccaacggtgt aagtgccgtc ggcttcttta cgcagccatt
     3421 cgtgttcttt gctgtatttc agttctgctg gtacgttgct catcaatcaa tctccaaaaa
     3481 agtaaatcac gcgacggctt tgccgttacg cacaaaaaca ggttttgtca ctttaaccga
     3541 catttcacgg ttgcgaattt gcacaatcgc cgtttcgcca ataccttcca gcacgcgcgc
     3601 cagcgcaatg ctgtaaccca gcgtcgggga gaaagtaccg ctggtgataa tgccttcatg
     3661 ctggttgccc tgcgcatcgg taaagcgtac cggcagttca ttacgcagca cgcctttttc
     3721 ggtcatcacc agaccaacca gtttttctgt accatgctca cgctgcactt ccagggcttc
     3781 acgaccgata aagtcacgat ctgccggttc ccaggcgata gtccagccca tgttggcggc
     3841 taaaggagaa atagtttcgt ccatctcctg accataaaga ttcatgcccg cttccagacg
     3901 cagcgtgtca cgcgcgccca agccacatgg cttaacaccc gcttccacca gcgcacgcca
     3961 gaaatcggcc gctttttcat tgggcagcgc aatttcatag cccgcttcac cggtataacc
     4021 agtggtggca ataaacagat cgcccgcctg cacgccaaag aacggtttca tcccttccac
     4081 cgcctgacgc tgggcgtcat taaacagtgt ggcagctttt gcctgcgcat tcggcccttg
     4141 cacggcaatc atggaaaggt catcacgaac ggtaatttcg atgccgaaag gttcagcgtg
     4201 ttgggtaatc caggagaggt ctttttcgcg ggtggcggag ttaacaacga ggcggaagaa
     4261 atcttcagta aagtagtaga cgatgaggtc atctatcaca ccgccagagg cattcaacat
     4321 ccccgagtaa agggctttgc cgcttttggt gagcttcgcc acatcgttcg ccagcagata
     4381 acgcagaaac tcccgggtgc ggctgccgcg gagatcgacg atggtcatat gtgacacatc
     4441 aaacattccg gcatcggtac gtaccgcatg atgttcgtcg atttgcgaac cgtaatgcag
     4501 cggcatcatc cagccgtgga aatccaccat gcgagcgccg caaagcgtgt gttgttcgta
     4561 caaaggagtc tgttgtgcca tcttgtcctc attgaataag cggggctgac aacttttcat
     4621 ggtgaaatta tcaccacgaa acccagcatc ggagccactc ccggtcccca acgcaatcgt
     4681 tctcttttgc ctgaacttac caccgaaaca gactgttaac cataaggtaa aattgatcat
     4741 cacattagct tatggttaaa aaacgccaaa atcgcgacag aataaaaaac caaaaaacac
     4801 accagtttct atataaagat gatgccatta gaaagctaat ttgaataagg caatattaag
     4861 agctaaaaaa atgtcaaaaa acactaaatc aaaaaataat gacattagaa aatataatgc
     4921 gaaaacggag gtgaaattag tttatttcaa atgaggaaaa tctcccggcg aaaaaaccgg
     4981 gagatgaaag tgtgatgggt atcaaataaa cagcagaaga gaaattttta acgcagccat
     5041 tcaggcaaat cgtttaatcc cattgcctgg cggataagtt gtggtttaac gccaggaagc
     5101 gtgtcggcca gtttcaaacc aatatcacgc agcagttttt tcgccggatt ggtaccggaa
     5161 aacagatcgc ggaatccctg cataccagcc agcatcagcg ccgcactgtg cttgcggcta
     5221 cgctcatacc ggcgcaggta aatgtactgc ccgatgtctt tcccctgacg atgcaaccgt
     5281 ttcagttcag caatcagctc tgcagcatcc ataaagccaa gatttactcc ctgccccgcc
     5341 agcgggtgaa tggtatgcgc ggcgtcgccc accagcacca gacggtgcgc ggcaaactgg
     5401 cgcgcataac gccccgtcag tgggaacacc tgacgcgcgc tctcaacctt gcataagccc
     5461 aggcgattat caaaagcgat atttaacgcg cgattaaatt catcttcact tgcctgctgc
     5521 atccgctgcg cttcctctgg cgacagtgac cagacaattg agcaaagatg cggatcgcta
     5581 agcggtaaaa aggccagaat gccttcgcca tggaaaacct gccgcgccac cgcatcatgc
     5641 ggttcttccg tgcgaatggt cgctaccagc gcgtgatgct gataatccca gaaagtcagc
     5701 ggaatatctg ctttgttgcg caaccaggaa ttagcaccgt ccgcgccaat caccagacgc
     5761 gccgttaaca tgctgccatc tttcagcgtc aggaaagttt cattttctcc ccaggcgacc
     5821 tgctgtaatt ctgcgggggc caacagagtg atatctgacg actgctgcgc tttgttccac
     5881 agcgcgtagt gaatcactga gttttcaacg atatgcccaa gatggctata gcccatgctt
     5941 tgatcgtcaa acgaaatgtg accaaaactg tctttgtccc acacttccat accgtgataa
     6001 caactggccc tacgagagag aatgtcctgc cagacgccaa gacgggtgag taatttttcg
     6061 ctggcggcat tgatagccga aacgcgcagt tgtggtggtg catccgccgc cagaggttcc
     6121 ggtacgcgct gctccagtac ggcaacgcgt aagccgctcc cctgtaagcc gcaggcaacc
     6181 gccagcccca ccatgccgcc accaacaatg gctacatcaa cactttgcat tgtttattcc
     6241 ttaaaaccgc cttcaacgcg ccacccaacc gagggtgcgc tgcgccagca catcgcgtgc
     6301 cggggtgaat aattccatcg tcatcagtcc gatgttgcgc ccgacaacca gcggtgccca
     6361 acggttggca aaaagatgta ccaggctgtc cgtgacgccg atggttgctt cgcgatcgct
     6421 ctgtcgacgc tgctgatagc ggcacaatat gccgtaatca ccgatgtctt ctccgcgctc
     6481 atgcgcctga gtcagggttt ccgcaaggct catgacatct cgcataccga ggttaaaccc
     6541 ttgcccggca atcgggtgca gagtttgcgc cgcattgccc accagcacgg tacgatgggt
     6601 aatagctctg gcggcgcggg ttaacgccag cggataagca ctgcgtttac cggcgtgggt
     6661 aatttttcca agtcgccagc caaaggccga ctggagttca cggcaaaact tctcgtcgct
     6721 ccacgacagc acctcttcgc gccgttccag tggatgacac cagaccagcg aacagcgtcc
     6781 gtcagacatc ggcaacatcg ccagcgggcc atgttgcgtg aagcgttcaa aagcgcggcc
     6841 ttcgtgcgca acggaagtcg caacgttggc aatcacggcc agttgttcgt aaggctcctg
     6901 ctgccagtca acgccgcacg cggtggctaa cgctgaatgg gtgccatcag ctgctaccag
     6961 cacgcggccc gtcagcgtct cgccactctc cagcgtcact tcaacgtgac tctgagtacg
     7021 ggcaacgtta gccacgcgat cagggcaatg cagcgttacg ccaggtgctt tacgcagcaa
     7081 tgcaaacagc cgttgcccga cattgtgcaa ttcgacaacc tgtcccagcg ccgccagttg
     7141 gtaatcttct gcggcgaggg tgacaaatcc agcgtgacca cgatcgctga catgcacggt
     7201 agtgatggcg gttgcgtaat ccgccagaga ttgccagacg ccgatgcgcg ccagttgctg
     7261 acaggtcccc gcagccagcg caatagctcg tccatcaaag cccggatgag catgtgactc
     7321 tggcgcggtc gcttcaatca aatgtaccgg cagcgccccg tgacttaacc gggaaatagc
     7381 cagcgccagc gtcgcgcccg ccatgccgcc accgacgatg attacgctca ttgctttctc
     7441 gcagctgcca tcaacgcttc gatttcttcc ggctttttca ccacgctggc ggtgaggttt
     7501 tcgttgccgg tttcggtaat cacaatgtcg tcttcaatac gaatgccgat accgcgatat
     7561 tgttctggca catccgcatc cggcgcgata tacagaccag gctctacggt cagtaccatc
     7621 cccggttcca gaatgcgcga gcgatcctga ccataaacgc caacgtcatg gacatccagt
     7681 cctaaccagt ggctaaggcc atgcataaag aaaggacgat gggcgttctg agcgatcagt
     7741 tcatcaacat cacctttcag gatgccgagt tttaccaggc cgctaaccat gatgcgcacc
     7801 acttcaccag tgacttccag aatggaagtt cccggacgat acaggcgcag gctggtttcg
     7861 agagactcca gcacaatgtc gtagatttca cgctgggcct gggtgaattt gccgttgacc
     7921 gggaaggtgc gggtaatatc gcccgcgtaa cctttgtatt cacaacccgc gtcaatcaac
     7981 accaggtcac cgtcgcgcag ttcactctcg ttttcggtgt agtgcagaat gcagccgttt
     8041 tcaccgctgc cgacaatggt gttataggac ggatagcgcg caccgtggcg gttaaattcg
     8101 tggtgaattt cgccttccag atggtactcg aacattcccg gacggcattt ttccatcgcc
     8161 cgtgtatgtg ccatggcggt gatttctccc gcgcggcgga gtacggcaat ctcttctggc
     8221 gatttgaaca ggcgcatttc atgaacaaca ggacgccagt cgatcatcgt tgccggtgcg
     8281 gtgagatttt gccgcgaacc tttacgcagt ttttccagcg cactgttcat gatttcatca
     8341 gcatatgcat attcgccctg ggcatggtaa accacatcca ggccgttaag tagttgataa
     8401 agttgctgat tgatttcgct gaatgccagt gcgcggtcaa cgcccagttt ctctggcgcg
     8461 gcatcctggc ctaagcgacg gccaaaccag atctccgccg tcaggtcgcg aacgcggtta
     8521 aacagaacgc tgtggttatg agtgtcatcg cttttaatca gcaccagcac cgcttccaat
     8581 tcgttaaagc cggtgaagta ccagaagtca ctgttctgac gataggggta ttcgctgtcg
     8641 gcgctacgtg ttacttctgg tgcagcaaaa atcagcgcgg cgctgccggg ttgcatttgc
     8701 tccaccaggg cctgacggcg acgctgaaac tcttgccggg atatctcact cataacactc
     8761 tccttacgtt ttttgttttt agtgtagagt cggtttttgt acttctggcg cggtcggttg
     8821 cggatgagta aaggtgtcgt ggcataacag cgcggcaaca cgaacgtatt cgatgatctc
     8881 ttcaagcgac atttcaagct cttcctgatc ttcgtcttcg tcgtaaccca gttgcgcaat
     8941 gttacgcaga tcgtcgatag cttcaccggt ttcgccggtc actttatcca gcttcggttg
     9001 cgtaacgcca agaccaagca ggaagtgatt gacccaacct gccaatgcat cagcccgatc
     9061 gaaaacgctg acatcatcgc catcaggcag ataaagctga aaaaggaagc cgtcatcctg
     9121 cagggcatcg ctggtggcag agtgcatttt acgcagtgcc tgtgccagct catgaccgaa
     9181 agccatgcct tcgttcgtca ggtcgtgaag tagcggcagc catgagctgt catcgttacc
     9241 gccacatatc atcccactga ttaaaccatg catctcggca ggggtcagcc ccgtcccttg
     9301 ttggttcaga tactggttca tttcgttgta accaggcatt tcgttctgta tagacataag
     9361 cattcgtcgt caaagggagg aatattcatg atatgctacc actttgggcc ctggtggacc
     9421 agaaaagggc ttgtctcttc tcatcagggt agctatagtg tcgccccttc gcagaccatg
     9481 ggtctaaaga cgaaggcagc gcagtcaatc agcaggaagg tggcatgtct gcacaacccg
     9541 tcgatatcca aatttttggc cgttcactgc gtgtgaactg cccgcctgac caaagggatg
     9601 cgttgaatca ggcagcggac gatctgaacc aacggttgca agatctgaaa gaacgcacta
     9661 gagtcacaaa tactgaacag ttggtcttca ttgccgcatt gaatatcagc tatgagttag
     9721 cgcaagaaaa agcgaagact cgtgactacg cggcaagtat ggaacagcgt attcggatgc
     9781 tgcagcagac catagaacaa gcgttacttg aacaaggtcg catcaccgaa aaaactaacc
     9841 aaaactttga atgacacttt tcggtttact gtggtagagt aaccgtgaag acaaaatttc
     9901 tctgagatgt tcgcaagcgg gccagtcccc tgagccgata tttcatacca caagaatgtg
     9961 gcgctccgcg gttggtgagc atgctcggtc cgtccgagaa gccttaaaac tgcgacgaca
    10021 cattcacctt gaaccaaggg ttcaagggtt acagcctgcg gcggcatctc ggagattccc
    10081 ttcttatctg gcaccagcca tgacgcaact accagaactc ccactgacat tatcccgaca
    10141 agaaatccgc aaaatgattc ggcaacgtcg tcgtgcgtta acgccggaac aacagcagga
    10201 aatgggtcaa caagccgcta cccggatgat gacttatccc ccggtggtga tggcgcatac
    10261 ggtcgctgta ttcctctctt ttgatggtga actcgacacc cagccgctaa tagaacaact
    10321 ctggcgcgcc ggtaagcgcg tatatcttcc agttttgcat ccctttagtg ccggtaattt
    10381 gctgttcctg aattaccatc cgcaaagcga actggtgatg aacaggttga agatccatga
    10441 gccaaaactg gatgtgcgtg acgtgctacc cctttcccga ttagacgtgc tgatcacacc
    10501 gctggtcgcc tttgatgagt acggtcagcg cctgggaatg ggcggtggtt tttatgatcg
    10561 gaccttacaa aactggcagc actataaaac gcaaccggtg ggttatgcgc atgattgtca
    10621 gttggtggaa aaactccccg ttgaagagtg ggatatccct cttcctgcgg tggttacacc
    10681 gtcgaaagtc tgggagtggt aagggcgata cacctgcatc gccctgattg acatcgttga
    10741 ttctttaacc taatttagtg agtaagggta agggaggatt gctcctcccc tgtgactgac
    10801 tgttaataag ctgggaaact tatgagtaac agtacaatca gtatgatggc aagtcgcatc
    10861 ataacccttc tccttcaagc cctcgcttcg gtgagggctt taccgttaca gccccatgct
    10921 gcccagctat cgtaagctct ctttaaataa ccacaacgca ttgatctacc tttaaatctt
    10981 ttcttcgagc gtgctcgaaa aacccatgca aaacagagat aatttttggg aaaaaggatc
    11041 tattacggca gtaaacgtgg gcgatgaagt ggctatcaaa aagttcggtt cagggcgatg
    11101 caccccgcat cgccctgatt gacatcgttg attctttgac ctaatttagt gagtaagggt
    11161 aagagaggat gactcctccc ctgagactga ctgttaataa gctccgaaac ttatcagtaa
    11221 cagtacaatc agtatgatga caagtcgcat cataaccctt ctcctttaag ccctcgcttc
    11281 ggtgagggct ttaccgttac agccccatgc tgtcccgcca tcgtaaatcc ccattaaata
    11341 aacacaacgc attgatctga gtttgattta ttttctggag cagactcgca aagtagaatg
    11401 cgcaacgcgg caacggtgtg gagaagggat aaaaaaacgg gcaagtcagt gacctgcccg
    11461 ttaattttca gagaagggga attagtacag cagacgggcg cgaatggtac ccggaatagc
    11521 tttcattgcc tgcagcgctt tttcggcaac gtcttcgtca gcttcaatat caataactac
    11581 ataacccatc tgggcggaag tttgtagata ttgcgcggcg atgttgacgc cctgctcggc
    11641 gaagattttg ttcagcgcag ttagcacgcc cggacggttt tcgtggatgt gcatcagacg
    11701 acgaccacca tgcagtggca gcgagacttc cgggaagttc accgcagaga gcgttgagcc
    11761 attgtcagaa tacttgatca atttacccgc aacttccagg ccgatattct cctgcgcttc
    11821 ctgagtcgaa ccgccaatgt gtggcgtcag aaggacgttg tcgaattcac acagtggaga
    11881 ggtaaatgga tcgctattgg tcgccggttc cgtcgggaat acgtcgattg ccgcccccgc
    11941 cagatgtttg ctcgccagcg catcacacag cgccggaata tccaccacag taccgcgcga
    12001 ggcattaatc agcagcgagc cgggcttcat tagcgaaatc tctttcgcgc ccatcatatt
    12061 tttggtggac ggattctctg gtacatgcag actcaccaca tcgctcatat tcagcaggtc
    12121 agaaagatgc tgtacctgag tggcgttgcc cagcggcagt ttgttttcaa tatcataaaa
    12181 gtaaacatac attcccagcg attcagccag aatgcccaat tgcgtaccaa tatgaccgta
    12241 gccgatgata cccagctttt tgccgcgcgc ttcaaaagaa cccgccgcca gtttgttcca
    12301 cacgccacgg tgcgctttag cattggcttc cggcacgccg cgcaatagca gcagcagttc
    12361 gccaatcacc agctccgcaa cagagcgcgt atttgagaac ggtgcgttaa ataccgggat
    12421 cccgcgcttt gccgccgcat ccagatcaac ctggtttgtt ccgatacaga aacagccaat
    12481 agcgaccagt ttttctgcgg cgttgatcac gtcttcagtc agatgggtac gggatcgcag
    12541 gccgatgaag tgggcatcgc ggatggattc ttttaattgt tcatcatcca gcgcgccttt
    12601 gtgaaattcg atgttggtgt aaccagctgc acgaaggctt tccagcgcct tttggtgcac
    12661 gccttctacc agcagaaact taatcttgtc tttctccagc gatacctttg ccatttaccc
    12721 aatcctgtct tttgaaatgt tgtgtgcgga tttgcatccg cctttcaaca tatcaaaaaa
    12781 taatattgcg gcaatatgaa cgtttgcgtc gcgatgttga ggaaatatca cgcaaaggta
    12841 tttcttggtt gaatatgctg gtttagagga tttatttaga gcaatcgaca atagccaagt
    12901 aaaaacgtga cacatgtcac caaatttaat gaagagaatt tttttaacgg gggaggttcc
    12961 cccgtcagat catttcacaa tggttttgac accgtcaggt gtgccaatca gcgcaacgtc
    13021 cgcgccacgg ttagcaaaca agccaacagt caccacgcca ggaatcgcat ttatggcgtt
    13081 ttccatcgct atcgggtcaa ggatttccat gccgtggacg tcgaggatca cgttgccgtt
    13141 atcggtcacc acgccctgac ggtattccgg acgaccgccc agtttcacca gctggcgcgc
    13201 cactgcacta cgtgccatcg ggataacttc tactggcagc gggaatttgc ccagaatatc
    13261 aacctgcttg gaagcgtcag caatacagat aaatttttcc gcaaccgaag caatgatttt
    13321 ttcacgggtc agcgccgcgc cgccgccttt gatcatttgc atgtggccgt tgatttcatc
    13381 tgcgccatca acgtagatgc caaggctgtc gacttcgttg agatcaaaaa cgtgaatgcc
    13441 gaggcttttc agtttttcag tggaagcatc tgaactggaa acggcccctt caatctggcc
    13501 tttcattgta ccgagcgcgt caataaagtg tgcggcggtg gaacctgtac ctacaccaac
    13561 aatggtgccg ggctgaacat actgaagtgc cgcccatcct actgcttttt tcaattcatc
    13621 ctgcgtcatg atcgtttcgc ctgtggtatg aaatttcaca cgcattatat acaaaaaaag
    13681 cgattcagac cccgttggca agccgcgtgg ttaactcatc cataaaatat cgcgcaatgg
    13741 cgggcatccc ctttcgcccc gcaaataaag catacaacgg tctgggtatg ccgctccacg
    13801 gtgcgaacag gcgcaccagt tccccccgtt cgcaagcccc tgtttacagg caaattgagg
    13861 caataacgcc accccattca acacaaccag ggcacgaact cgctgagcga aatgcattag
    13921 cgcaaatccc tgatctttta agctgcgcgt ttaaccacgg atacaaatgt tacccgccga
    13981 cggcttcggt atatgcaacc tgacacaaaa ttgtgtcata gtgcaggaaa aagcatttac
    14041 caggagcaga caacagcaat gaaacgcccg gactacagaa cattacaggc actggatgcg
    14101 gtgatacgtg aacgaggatt tgagcgcgcg gcacaaaagc tgtgcattac acaatcagcc
    14161 gtctcacagc gcattaagca actggaaaat atgttcgggc agccgctgtt ggtgcgtacc
    14221 gtaccgccgc gcccgacgga acaagggcaa aaactgctgg cactgctgcg ccaggtggag
    14281 ttgctggaag aagagtggct gggcgatgaa caaaccggtt cgactccgct gctgctttca
    14341 ctggcggtca acgccgacag tctggcgacg tggttgcttc cggcactggc tcctgtgttg
    14401 gctgattcgc ctatccgcct caacttgcag gtagaagatg aaacccgtac tcaggaacgt
    14461 ctgcgccgcg gcgaagtggt cggcgcggtg agtattcaac atcaggcgct gccgagttgt
    14521 cttgtcgata aacttggtgc gctcgactat ctgttcgtca gctcaaaacc ctttgccgaa
    14581 aaatatttcc ctaacggcgt aacgcgttcg gcattactga aagcacctgt ggtcgccttt
    14641 gaccatcttg acgatatgca ccaggccttt ttgcagcaaa acttcgatct gcctccaggc
    14701 agcgtgccct gccatatcgt taattcttca gaagcgttcg tgcaacttgc tcgccagggc
    14761 accacctgct gtatgatccc gcacctgcaa atcgagaaag aactagccag cggtgaactg
    14821 attgacttaa cgccagggct atttcaacgc cggatgctct actggcaccg ctttgctcct
    14881 gaaagccgca tgatgcgtaa agtcactgat gcgttgctcg attatggtca caaagtcctt
    14941 cgtcaggatt aatccatcaa ataatgcctg atagcacata tcaggcgtta tcctcacttc
    15001 tttttgtatt ccttgaatca catcacaaaa tagacaaatc tcaggcggca aaaaacgacg
    15061 tctgaatgca tttttttgct ggcgacaaac ccacgtaaaa agctcaccgt aagcgcaaat
    15121 accctcattt tgattgcgtt ttacggagca aataatgtct aacgagcagg agtggcaaca
    15181 gcttgccaac aaggaattga gccgtcggga gaaaactgtc gactcgctgg ttcagcaaac
    15241 cgcggaaggg atcgccatca agcagctgta taccgaagcc gatctcgata atctggaggt
    15301 gacaggtacc cttcctggtt tgccgcccta cgttcgtggc ccgcgtgcca ctatgtatac
    15361 cgcccaaccg tggaccatcc gtcagtatgc tggtttttca acagcaaaag agtccaacgc
    15421 tttttatcgc cgtaacctgg ccgccgggca aaaaggtctt tccgttgcgt ttgaccttgc
    15481 cacccaccgt ggctacgact ccgataaccc acgcgtggcg ggtgacgtcg gcaaagcggg
    15541 cgtcgctatc gacaccgtgg aagatatgaa agtcctgttc gaccagatcc cgctggataa
    15601 aatgtcggtt tcgatgacca tgaatggcgc agtgctacca gtactggcgt tttatatcgt
    15661 cgccgcagaa gagcaaggtg ttacacctga taaactgacc ggcaccattc aaaacgatat
    15721 tctcaaagag tacctctgcc gcaacaccta tatttaccca ccaaaaccgt caatgcgcat
    15781 tatcgccgac atcatcgcct ggtgttccgg caacatgccg cgatttaata ccatcagtat
    15841 cagcggttac cacatggggg aagcgggtgc caactgcgtg cagcaggtag catttacgct
    15901 cgccgatggg attgagtaca tcaaagcagc aatctctgcc ggactgaaaa ttgatgactt
    15961 cgctcctcgc ctgtcgttct tcttcggtat cggcatggat ctgtttatga acgtcgccat
    16021 gttgcgtgcg gcacgttatt tatggagcga agcggtcagt ggatttggcg cacaggatcc
    16081 gaaatcactg gcgctgcgta cccactgcca gacctcaggc tggagcctga ctgaacagga
    16141 tccgtataac aacgttatcc gcaccaccat tgaagcactg gctgcgacgc tgggcggtac
    16201 tcagtcactg cataccaacg cctttgacga agcgcttggt ttgcctaccg atttctcagc
    16261 acgcattgcc cgcaacaccc agatcatcat ccaggaagaa tcagaactct gccgcaccgt
    16321 cgatccactg gccggatcct attacattga gtcgctgacc gatcaaatcg tcaaacaagc
    16381 cagagctatt atccaacaga tcgacgaagc cggtggcatg gcgaaagcga tcgaagcagg
    16441 tctgccaaaa cgaatgatcg aagaggcctc agcgcgcgaa cagtcgctga tcgaccaagg
    16501 caagcgtgtc atcgttggtg tcaacaagta caaactggat cacgaagacg aaaccgatgt
    16561 acttgagatc gacaacgtga tggtgcgtaa cgagcaaatt gcttcgctgg aacgcattcg
    16621 cgccacccgt gatgatgccg ccgtaaccgc cgcgttgaac gccctgactc acgccgcaca
    16681 gcataacgaa aacctgctgg ctgccgctgt taatgccgct cgcgttcgcg ccaccctggg
    16741 tgaaatttcc gatgcgctgg aagtcgcttt cgaccgttat ctggtgccaa gccagtgtgt
    16801 taccggcgtg attgcgcaaa gctatcatca gtctgagaaa tcggcctccg agttcgatgc
    16861 cattgttgcg caaacggagc agttccttgc cgacaatggt cgtcgcccgc gcattctgat
    16921 cgctaagatg ggccaggatg gacacgatcg cggcgcgaaa gtgatcgcca gcgcctattc
    16981 cgatctcggt ttcgacgtag atttaagccc gatgttctct acacctgaag agatcgcccg
    17041 cctggccgta gaaaacgacg ttcacgtagt gggcgcatcc tcactggctg ccgatcataa
    17101 aacgctgatc ccggaactgg tcgaagcgct gaaaaaatgg ggacgcgaag atatctgcgt
    17161 ggtcgcgggt ggcgtcattc cgccgcagga ttacgccttc ctgcaagagc gcggcgtggc
    17221 ggcgatttat ggtccaggta cacctatgct cgacagtgtg cgcgacgtac tgaatctgat
    17281 aagccagcat catgattaat gaagccacgc tggcagaaag tattcgccgc ttacgtcagg
    17341 gtgagcgtgc cacgctcgcc caggccatga cgctggtgga aagccgtcac ccacgtcatc
    17401 aggcgctaag tacacagctg cttgacgcca ttatgccgtt ctgtggtaac gccctgcgac
    17461 tgggcgttac cggcacaccc ggcgcgggga aaagtacctt tcttgaagcc tttggcatgt
    17521 tgttgattcg tgagggatta aaggttgctg ttattgcggt cgatcccagc agcccggtca
    17581 ctggcggtag cattctcggg gataaaaccc gcatgaatga cctggcgcgt gccgaggcgg
    17641 cgtttattcg cccggtaccc tcctccggtc atctgggcgg tgccagtcag cgagcacggg
    17701 aattaatgct gttatgcgaa gcggcgggtt atgacgtagt gattgtcgaa acggttggcg
    17761 tcgggcagtc ggaaacggaa gtcgcccgca tggtggactg ttttatctcg ttgcaaattg
    17821 ccggtggcgg cgatgatctg cagggcatta aaaaagggct gatggaagtg gctgatctgc
    17881 tcgttatcaa caaagacgat ggcgataacc ataccaatgt cgccattgcc cggcatatgt
    17941 acgagagtgc cctgcatatt ctgcgacgta aatacgacga atggcagcca cgggttctga
    18001 cttgtagcgc actggaaaaa cgtggaatcg atgagatctg gcacgccatc atcgacttca
    18061 aaaccgcgct aactgccagt ggtcgtttac aacaagtgcg gcaacaacaa tcggtggaat
    18121 ggctgcgtaa gcagaccgaa gaagaagtac tgaatcacct gttcgcgaat gaagatttcg
    18181 atcgctatta ccgccagacg cttttagcgg tcaaaaacaa tacgctctca ccgcgcaccg
    18241 gcctgcggca gctcagtgaa tttatccaga cgcaatattt tgattaaagg aatttttatg
    18301 tcttatcagt atgtgaacgt tgccactatc aacaaagtgg cggtcattga gtttaactat
    18361 ggacgaaaac ttaatgcctt aagtaaagtc tttattgatg atcttatgca ggcgttaagc
    18421 gatctcaacc ggccggaaat tcgctgtatc attttgcggg cgccaagtgg atccaaagtc
    18481 ttctccgcag gtcacgatat tcacgaactg ccgtctggcg gtcgcgatcc gctctcctat
    18541 gatgatccat tgcgtcaaat cacccgcatg atccaaaaat tcccgaaacc gatcatttcg
    18601 atggtggaag gtagtgtttg gggtggcgca tttgaaatga tcatgagttc cgatctgatc
    18661 atcgccgcca gtacctcaac cttctcaatg atgcctgtaa acctcggcgt cccgtataac
    18721 ctggtcggca ttcacaacct gacccgcgac gcgggcttcc acattgtcaa agagctgatt
    18781 tttaccgctt cgccaatcac cgcccagcgc gcgttggctg tcggcatcct caaccatgtt
    18841 gtggaagtgg aagaactgga agatttcacc ttacaaatgg cgcaccacat ctctgagaaa
    18901 gcgccgttag ccattgctgt tatcaaagaa gagctgcgtg tactgggcga agcgcacacc
    18961 atgaactccg atgaatttga acgtattcag gggatgcgcc gcgcggttta tgacagcgaa
    19021 gattaccagg aagggatgaa cgctttcctc gaaaaacgta aacctaattt cgttggtcat
    19081 taatccctgc gaacgaagga gtaaaaatgg aaactcagtg gacaaggatg accgccgatg
    19141 aagcggcaga aattatccag cataacgaca tggtggcatt tagcggcttt accccagcgg
    19201 gttcgccgaa agccttaccg accgcgattg cccgcagagc taacgaacag catgaggcca
    19261 aaaagccgta tcaaattcgc ctgctgacgg gggcgtcaat cagcgccgcc gctgacgatg
    19321 tactttctga cgccgatgct gtttcctggc gtgcgccata tcaaacatcg tccggtttac
    19381 gtaaaaagat caatcagggc gcggtgagtt tcgttgacct gcatttgagc gaagtggcgc
    19441 aaatggtcaa ttacggtttc ttcggcgaca ttgatgttgc cgtcatcgaa gcatcggcac
    19501 tggcaccgga tggtcgagtc tggttaacca gcgggatcgg taatgcgccg acatggctgc
    19561 tgcgggcgaa gaaagtgatc attgaactta atcactatca cgatccgcgc gttgcagaac
    19621 tggcggatat tgtgattcct ggcgcaccac cgcggcgcaa tagcgtgtcg atcttccatg
    19681 caatggatcg cgtcggtacc cgctatgtgc aaatcgatcc gaaaaagatt gtcgccgtcg
    19741 tggaaaccaa cttgcccgac gccggtaata tgctggataa gcaaaatccc atgtgccagc
    19801 agattgccga taacgtggtc acgttcttat tgcaggaaat ggcgcatggg cgtattccgc
    19861 cggaatttct gccgctgcaa agtggcgtgg gcaatatcaa taatgcggta atggcgcgtc
    19921 tgggggaaaa cccggaaatt cctccgttca tgatgtattc ggaagtgcta caggaatcgg
    19981 tggtgcattt actggaaacc ggcaaaatca gcggggccag cgcctccagc ctgacaatct
    20041 cggccgattc cctgcgcaag atttacgaca atatggatta ctttgccagc cgcattgtgt
    20101 tgcgtccgca ggagatttcc aataacccgg aaatcatccg tcgtctgggc gtcatcgctc
    20161 tgaacgtcgg cctggagttt gatatttacg ggcatgcaaa ttcaacgcac gtagccgggg
    20221 tcgatctgat gaacggcatc ggcggcagcg gtgattttga acgcaacgcg tatctgtcga
    20281 tctttatggc tccgtcgatt gctaaagaag gcaagatctc aaccgtcgtg ccaatgtgca
    20341 gccatgttga tcacagcgaa cacagcgtca aagtgatcat caccgaacaa gggatcgccg
    20401 atctgcgcgg tctttccccg cttcaacgcg cccgcactat cattgataat tgtgcacatc
    20461 ctatgtatcg ggattatctg catcgctatc tggaaaatgc gcccggcgga catattcacc
    20521 acgatcttag ccacgtcttc gacttacacc gtaatttaat tgcaaccggc tcgatgctgg
    20581 gttaatggtt tgtcccgtca cgatcttcag caatatgctg aagcatcgtg gcggtataac
    20641 gatgatttct gattgaataa agatattcct gcatatacat cggcctgtgc ggcgcatcaa
    20701 aatatttcag cgttgccgga ttcaccagtc gccaggcaaa atgcgggatc accgtcagaa
    20761 aatgtccgcg ctccacggcg ctgattttgg ccataaaact gtagggacga taaataatgg
    20821 tgggattgat cccacaaggt cgcatattcg catcaatcat tgcttcgaag ttagcccggt
    20881 tttgaaagcg catttgtagc cagggcagct cctgaagcag ctcttgctct tgacgctctt
    20941 cataacggcg agaaacgaga aagcccaatc gcagcggtgg taattcactg atagtcaggt
    21001 tttccagttc ctgaactcgt gcagatacat gctgtgggga gataataaaa tctaactgac
    21061 ggtcaaaaag attatcgata acgccgtttt cgctgaactc aactggctgg gcggtcacgc
    21121 cttcatactt atctccgaga ctaatcaact gatcgaaaat aattgtcgga taagtattat
    21181 ctatgccaat cacaatt
//
LOCUS       DAZPAC010000055        20711 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZPAC010000055 DAZPAC010000000
VERSION     DAZPAC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.055"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHUPM_001748"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHUPM_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR0869547.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHUPM_001749"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHUPM_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR0869548.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHUPM_001750"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHUPM_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR0869549.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHUPM_001751"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHUPM_001751"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR0869550.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="ACHUPM_001752"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="ACHUPM_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR0869551.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="ACHUPM_001753"
     CDS             complement(8214..9107)
                     /locus_tag="ACHUPM_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR0869552.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHUPM_001754"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHUPM_001754"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR0869553.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHUPM_001755"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHUPM_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR0869554.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHUPM_001756"
     CDS             complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHUPM_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR0869555.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHUPM_001757"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHUPM_001757"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR0869556.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHUPM_001758"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHUPM_001758"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR0869557.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16539)
                     /locus_tag="ACHUPM_001759"
     CDS             complement(14275..16539)
                     /locus_tag="ACHUPM_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFR0869558.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="ACHUPM_001760"
     CDS             complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="ACHUPM_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR0869559.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="ACHUPM_001761"
     CDS             complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="ACHUPM_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR0869560.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="ACHUPM_001762"
     CDS             complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="ACHUPM_001762"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR0869561.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="ACHUPM_001763"
     CDS             complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="ACHUPM_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR0869562.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4971 a         5732 c         5044 g         4964 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttcctg
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaagcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacacc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgct atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    15181 ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc agaaaagtaa
    15241 agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg gcccgcttaa
    15301 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15361 taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcacggg
    15421 cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15481 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15541 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15601 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15661 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15721 ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc agtggatcca
    15781 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acaccgcaaa
    15841 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15901 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15961 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    16021 attcggttca ataagacttg cctgcagaaa gcggccggtg agcggctgat gctgggcaat
    16081 ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg cacgaacttt
    16141 gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt cgccatacag
    16201 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16261 agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16321 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16381 cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc aggcgaagag
    16441 agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca acggaaaaat
    16501 tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc tgtttatgga
    16561 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16621 cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16681 ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16741 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16801 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16861 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16921 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16981 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    17041 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    17101 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    17161 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    17221 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17281 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17341 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt agcgtcttca
    17401 acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17461 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17521 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17581 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17641 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17701 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17761 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17821 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17881 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17941 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    18001 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    18061 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    18121 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    18181 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18241 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18301 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18361 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18421 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18481 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18541 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18601 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18661 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18721 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18781 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18841 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18901 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18961 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    19021 tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggat gccggaacca
    19081 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    19141 gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    19201 cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc accgttccca
    19261 tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19321 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc acttcctgag
    19381 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggttg
    19441 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct gacgcaacat
    19501 caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc gaatccagca
    19561 gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19621 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19681 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19741 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19801 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat g
//
LOCUS       DAZPAC010000056        20474 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZPAC010000056 DAZPAC010000000
VERSION     DAZPAC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20474
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.056"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1133
                     /gene="ypdA"
                     /locus_tag="ACHUPM_001764"
     CDS             <1..1133
                     /gene="ypdA"
                     /locus_tag="ACHUPM_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR0869563.1"
                     /translation="LVQSVEGEKEASAARQTKLALDIANKTLPLFRHVNSESLRKVCE
                     IIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEA
                     HRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRA
                     EQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE
                     LKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAI
                     VHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNV
                     HHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1148..1882
                     /gene="ypdB"
                     /locus_tag="ACHUPM_001765"
     CDS             1148..1882
                     /gene="ypdB"
                     /locus_tag="ACHUPM_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR0869564.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1895..2752
                     /locus_tag="ACHUPM_001766"
     CDS             1895..2752
                     /locus_tag="ACHUPM_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0869565.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2755..5250)
                     /gene="ptsP"
                     /locus_tag="ACHUPM_001767"
                     /pseudo
     CDS             complement(2755..5250)
                     /gene="ptsP"
                     /locus_tag="ACHUPM_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5275..6312)
                     /gene="ypdE"
                     /locus_tag="ACHUPM_001768"
     CDS             complement(5275..6312)
                     /gene="ypdE"
                     /locus_tag="ACHUPM_001768"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR0869566.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6312..7397)
                     /gene="ypdF"
                     /locus_tag="ACHUPM_001769"
                     /pseudo
     CDS             complement(6312..7397)
                     /gene="ypdF"
                     /locus_tag="ACHUPM_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7412..8659)
                     /locus_tag="ACHUPM_001770"
     CDS             complement(7412..8659)
                     /locus_tag="ACHUPM_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR0869567.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8681..9007)
                     /locus_tag="ACHUPM_001771"
     CDS             complement(8681..9007)
                     /locus_tag="ACHUPM_001771"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR0869568.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9227..10192)
                     /gene="glk"
                     /locus_tag="ACHUPM_001772"
     CDS             complement(9227..10192)
                     /gene="glk"
                     /locus_tag="ACHUPM_001772"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR0869569.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10396..11652
                     /locus_tag="ACHUPM_001773"
     CDS             10396..11652
                     /locus_tag="ACHUPM_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR0869570.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11767..12093
                     /gene="ypeC"
                     /locus_tag="ACHUPM_001774"
     CDS             11767..12093
                     /gene="ypeC"
                     /locus_tag="ACHUPM_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR0869571.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12140..13378)
                     /locus_tag="ACHUPM_001775"
     CDS             complement(12140..13378)
                     /locus_tag="ACHUPM_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR0869572.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13714..14916
                     /gene="nupC"
                     /locus_tag="ACHUPM_001776"
     CDS             13714..14916
                     /gene="nupC"
                     /locus_tag="ACHUPM_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR0869573.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(14966..17154)
                     /locus_tag="ACHUPM_001777"
                     /pseudo
     CDS             complement(14966..17154)
                     /locus_tag="ACHUPM_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(17363..17438)
                     /locus_tag="ACHUPM_001778"
     tRNA            complement(17363..17438)
                     /locus_tag="ACHUPM_001778"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17403..17405),aa:Ala,seq:ggc)
     gene            complement(17478..17553)
                     /locus_tag="ACHUPM_001779"
     tRNA            complement(17478..17553)
                     /locus_tag="ACHUPM_001779"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17518..17520),aa:Ala,seq:ggc)
     gene            17774..18133
                     /locus_tag="ACHUPM_001780"
     CDS             17774..18133
                     /locus_tag="ACHUPM_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0869574.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18135..18527
                     /locus_tag="ACHUPM_001781"
     CDS             18135..18527
                     /locus_tag="ACHUPM_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0869575.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18579..19994)
                     /gene="gltX"
                     /locus_tag="ACHUPM_001782"
     CDS             complement(18579..19994)
                     /gene="gltX"
                     /locus_tag="ACHUPM_001782"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR0869576.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20253..20328
                     /locus_tag="ACHUPM_001783"
     tRNA            20253..20328
                     /locus_tag="ACHUPM_001783"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20286..20288,aa:Val,seq:tac)
     gene            20373..20448
                     /locus_tag="ACHUPM_001784"
     tRNA            20373..20448
                     /locus_tag="ACHUPM_001784"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20406..20408,aa:Val,seq:tac)
BASE COUNT         5087 a         5460 c         5086 g         4841 t
ORIGIN      
        1 ttctggtgca aagcgttgag ggcgaaaaag aggccagcgc cgcgcggcag accaagctgg
       61 cgctggatat cgccaacaaa acgctaccgc tgtttcgcca tgtcaatagc gagtcattac
      121 gcaaggtctg cgaaattatc cgcgatgaca ttcacgccga tgcggtggcg attactaata
      181 ccgatcatgt gctggcctat gttggcgtgg gtgaacataa ctatcagaat ggcgatgact
      241 tcattagccc gactacccgt caggcgatga attacggaaa aatcatcatt aaaaacaatg
      301 atgaagccca ccgcacacca gagattcatt ccatgctggt gatcccattg tgggagaaag
      361 gggtcgtgac cggaacgctg aaaatttact actgccacgc gcatcagatc acctcgtcat
      421 tacaggaaat ggcggtcggt ctgtcgcaaa tcatctccac gcaactggag gtttcacgcg
      481 ccgagcagct acgtgaaatg gcaaataagg cagagcttcg cgccctgcaa agcaaaatta
      541 atccccattt tctgtttaac gctctgaacg ctatttcatc gtcaatccgt ctgaatccgg
      601 ataccgctcg ccagttgatc tttaatctgt cgcgttatct gcgctataac attgaattaa
      661 aagacgatga gcaaatcgat atcaaaaaag agctgtatca aattaaagac tatattgcca
      721 ttgagcaggc ccgctttggt gacaagctga cggttatcta tgatattgat gaagaggtga
      781 attgctgcat tcccagcctg ctgatccagc cgttggtgga gaacgccatt gtccacggta
      841 ttcagccttg caaaggtaaa ggcgttgtca ccatcagcgt tgcagagtgc ggaaatcggg
      901 tacgcattgc ggtgcgagat accgggcacg gcatcgatcc aaaggtgatt gagcgggtcg
      961 aagcgaatga aatgccgggc aataaaattg gcctgctgaa tgtccatcat cgcgtgaagt
     1021 tattgtatgg cgaggggctg catatccgcc gcctggagcc ggggacggaa attgcgtttt
     1081 acattcctaa ccaacgcacc ccagtcgcct cacaggctac gttattgctt tgagccggag
     1141 tgatattgtg aaagtcatca ttgttgaaga cgaattcctg gcacaacagg aactgagctg
     1201 gctaattaaa gagcacagcc agatggagat tgtcggcacc tttgacgacg gtctggacgt
     1261 gttgaagttt ttgcagcata accgcgtcga cgccattttt ctggatatca atattccgtc
     1321 gctggatggc gtgttgctgg cgcaaaacat cagccagttc gcccataaac cgtttattgt
     1381 gttcatcacc gcgtggaaag aacatgcggt agaagcgttt gaactggagg cgtttgacta
     1441 cattctcaaa ccgtatcagg agtcacgtat taccgggatg ctgcaaaaac tggaagcggc
     1501 ctggcaacaa cagcagacca gcagtacgcc tgccgcgacg gtaacgcgtg agaatgacac
     1561 cattaatctg gtgaaagatg agcgaataat cgtcacgcca attaacgata tctattacgc
     1621 cgaagcgcac gagaaaatga cctttgtcta tacgcggcgt gaatcctacg taatgccgat
     1681 gaacattacc gaattttgca gcaaactgcc gccgtcgcat tttttccgct gccatcgctc
     1741 attttgtgtc aatctgaaca aaatacgcga aatcgaaccg tggtttaata acacctacat
     1801 tctgcgactg aaagatctgg attttgaagt gccggtcagc cgcagcaaag tgaaagaatt
     1861 tcgccagtta atgcatcttt aatcgggaac tttcatgaaa gcaccaggct tgcccgccga
     1921 tcaacaattt ttcgccgatc tgttcagcgg cctggtgctt aacccgcaac tactggggcg
     1981 cgtctggttt gccagccagc ctgcctcgtt accggtgggc agtttatgta ttgattttcc
     2041 ccgtctggat atcgtgctgc gcggcgaata cggcaacctg ctggaagcaa agcagcagcg
     2101 aatggtggaa ggagaaatgc tgtttattcc ggcacgcgcg gctaatttac cgatcaacaa
     2161 caaaccggtg atgctgttaa gcctggtgtt cgcaccgacc tggcttgggt tatcgtttta
     2221 cgatagccgc accacgtcgt tgttgcatcc tgctcgccag atccagcttc ccagcctgca
     2281 acgcggtgaa ggtgaagcga tgcttaccgc cctcacccat cttagccgtt cgccgctgga
     2341 gcagaatatc attcagccac tggtgttaag tttgctgcat ctttgccgca acgtggtgaa
     2401 tatgccgccg ggtaattcgc aaccgcgcgg cgattttctc tatcacagca tttgtaactg
     2461 ggttcaggat aattatgccc agccgctcac ccgcgagagc gtggcgcagt tttttaatat
     2521 cacgcccaat catctgtcaa aactgtttgc tcagcatgga acgatgcgtt ttatcgagta
     2581 tgtgcgttgg gtgcgaatgg cgaaggcgag gatgattttg cagaaatatc atctgtcaat
     2641 tcatgaagtg gcacagcgtt gcggttttcc ggatagcgac tatttttgtc gcgttttccg
     2701 gcgtcagttt ggtctgacgc cgggagagta cagcgcccgt tttcagggct aacgtcagaa
     2761 ggttaattct gtttccagca gcgtcaggat actttgtgca tcttgcgcgg caaacaacga
     2821 ctggcggaag tttttattca ccagtttacg cgccagctgc gagaagactt tcacatgatt
     2881 catcccttcg ttagcaccca gcgtcagcat gatcaccagt tcaacttcgc ccatttctga
     2941 ctgccagtca accggtttcg ccagccgggc aatgctgata ctggaatgac ggatccactg
     3001 agatttggtg tgcgggatcg ctacgccaaa accaacgccg gtggtaacaa tctcttcccg
     3061 ctgccagaca tcttcttcca gctcaaacgg atgttcagtg cgcccgttaa cgccgaggtt
     3121 gccgcacagg aactggatcg cctgctcttt attgctaaaa tcctgatcaa caaagatatt
     3181 ctccagcgcc agcagtgggc gaacgtcttc ttccggcgta aaggcggtga gtaacgcttc
     3241 aatttcctgc gcactgcggc attcacatgc ctgacgcgcc agttcccgac acgcctcgct
     3301 atccagttga cgaagctggc ttttcaccgc cggaatacgc gggctactca tactcagctc
     3361 atccaggccc agcccaagca gtagcggcag ataacggctt tcaccgccca gttcaccgca
     3421 aatgcctacc catttgcccc gctgatgcgc agtggtaact atttgctgca acatgcgcag
     3481 gaacgatggc gtaatcgggt tatatagcgg cgatacgcgc gggttattac gatcgaccgc
     3541 atacagatac tgggtcatat cgttggagcc gatactgaag aaatcgacct catcgcagaa
     3601 gtggtcgatg atgtagcaca ccgacggcac ttccaccatg atcccaagcg taatcgtctc
     3661 tgcatgacgc aggccatcgc gcttaagctc aacgatcgct ttttggatct cgcctttcac
     3721 ccataagatc tgatcgaggc tgttaaccat cgggatcatc aactgggcgt tgccgaaact
     3781 ggcggcgcgc aaaatggccc gcagttgagt gcggaacagg ccagcaaatt ccgggtaaat
     3841 acgtaccgcg cgatagccga ggaacgggtt ctcttcctgg ggaatattca gataaggaat
     3901 gcttttatcg ccgccgatgt ccatcgtgcg gaagataatc ggcttgtcgc ccgccgccag
     3961 tagcacctgc tggtaggctt caaattgctc ctgctcgtcc ggcgcgctgt cgcgatccat
     4021 atagagcatt tcggtacgga acagcccgac accttccgcg ccgttggcaa acgcgcctgg
     4081 cgcttccaga gcggtgccga tattcgccgc aatatcaata cgcttgttat cacgggaata
     4141 ggccagctgc gcggcagcct gcgcctgttg tttttggcgt ttatccgcca gcgtctgcgc
     4201 gacctgataa taaccgctca ccgcgtcatt cgggttaatc gccagcacgc cgcactgggc
     4261 gtcaagcact gcaggttgcc cggcataacg ggcaatcgca tccagcggca agccactcag
     4321 taccgggatc gccgaggcac gggccagaat cagtgtatgc gaggtgcgcc cggttttctc
     4381 cagaatcatg cccgcaagat ttttcaaatc aaggctcaaa aactggcttg gggttaaatc
     4441 ttcagccacc agaatggtcg gtttttccag caccagattg ttgcgcggct tcagttccgg
     4501 ctaggtgata tgcagcaact gttcgctgat atcgcgaatg tcgctgaccc gctcgcgcag
     4561 ataatcgctg gcagaagcag atagtttggc gcaaacctgc tccatattgc tgatgatcgc
     4621 cgcccccagt ccctgatgct gttctgtcat caggcgacgg atattgcctg caaattcatc
     4681 atcctgaatc agcgacaaat gagcgctgag gatagttttg ctttcgccgt cacgctcacg
     4741 caattgctgg ttcagttgct cggcaagcgt tgccaggctg tgctccagcc gggtggaatc
     4801 ttgcgcactg gcggggattg cccgataact gtcgaggctg tcgctttgta acagggtcag
     4861 cgtacccacg ccgacgccgc ttgccagcac attgccgtac agtaaatccg ggttaaggcg
     4921 gcttaatgaa cgcggcagcg gatgcgccgt cagttctgcc tgcgtcggct gaacgctgtc
     4981 gctgtcgata aagcgcacca ggatgtactc ttccagcacg cgccgcgcct gctcttcatc
     5041 gctgccgcta atgttcaggc tgcaactgtc attaaataag gtgccagttc caatcaacgc
     5101 cagcgagctt ttagcatcgg cctttgcgtt ctggcgatgg ttgataaagg tgatttcgct
     5161 ttgccactgg ctgcactgtt ctttaagttc ccacgccgga cgagcatgta gaccgttagg
     5221 caaaggacag agaaattgaa tcgttaacat aaccgctccg ggttaatcag gagatcatct
     5281 gaaatccgtc agttgaacaa ccgtctcacg cgtaagacgt tgaataaggg cagataaaag
     5341 ttgctccatc tgcaaaatgt cgcggcaatc ggcaatcgat gccgcgcaat gtccgtggcg
     5401 ggtggcaggc cccattacca ctgtgggtac gccagtatcg gttaagtgca ccgctccacc
     5461 gtccgtgccg ccgttactga acatatccgc ctgcaacgga acgccaattt ctgccgacac
     5521 ggtttcaatc cacgcggtaa gttttggcgg cgcaatcagc gatttatcgc ttaacaccag
     5581 catcggcccg ttaccaatct gacgatggtt agccgcgcca taatcaaagt ttttcgccca
     5641 gcaggcggta tcaagcacaa tggcgacgtc cggcgacacc gcgcgggtag cagtttgccc
     5701 gccgcgtaat cccacctctt cgctggaact tgccaccagc cacacttccg caggcagttc
     5761 ggcgtcgtgc agctcacgta gtaacgtcac cagcagatag caaccgaggc ggtcatcaaa
     5821 ggctttcccc atcacccgct ggtgagggag aacctgaaaa gctgtatcaa aggtgacgcg
     5881 atcgcctgga cgaattcccg cctgcatcac ttcgtcatac gagcgcgcac caatgtccac
     5941 gcgcatggcg ctgacgtcat tcccctgccg gtcgccgtca agcaggcctg gaattttgca
     6001 ctcttcacgg gtggtgatgc gcaccggctg cagctggcgg gcagccatgc gcacgttgcc
     6061 aaccggcagc acatcaattg ccccttcgcg ggagatgctg cgcaccataa atcccacttc
     6121 gtccatatgc gcacagatca tcaccttcgg acctgtcgat tcattgaggc ggatcagcac
     6181 cgatcccaga ccatcaaatc gtacttcttt ttgcaggcga tccgcttctt ccagtaggat
     6241 ctgccgcact tcctgttccg aggaggcgat cgcatctgcc tcgctcaacg cttttaatag
     6301 cgataaatcc attatgcctc tcccgtgagc aacactgttt tcggcatggc gtagagcact
     6361 tctgcgcctt gcggggtaac cagcacaaca tcttcgatgc gcacgccccc ttgccctggc
     6421 aaataaatcc ccggctccac ggtcagtaac atgcctggct gtagcgtcgt ggtatcccgc
     6481 ggtgaaaagc gtggatcttc gtgtacttca ataccgatag catgaccggt gttgtgacca
     6541 aaatagtcgc caaatcctgc ctcggtaatc acccggcgcg cggcgtcgtc aacctgttag
     6601 cagcgtacgc cagggcgaat cgcggagatt gccgccagct gtgcttgcag gacaatctga
     6661 tagacgttaa acagcgggtg agattcggca attacccctt cgccattcac cagcaaggtg
     6721 cgcgtcatat cagagcagta cccctgatag agcgcaccaa aatcaagagt gacaaactcg
     6781 cctgctgcaa caatcttgtc gctggctttg ccgtgcggca gcgccccacg ccagccgctg
     6841 gcgacaatgg tgtcaaaaga ggctttttct gcgccctgct ggcgcataaa ccactccagt
     6901 tcagcggcta tctcgcgctc gctcatcccc gcctgaataa agcggcgaat atgctccgct
     6961 ccgcgatcgg caatcccaca ggcgaggcgg attatctcca cctcctctgg cgttttgatt
     7021 tgccgcagca catccggcgt ggcgctaacc agtttcgcat tgagttcaga cttccagcgg
     7081 tgcgcggttt cccaactcac ctgctggcct tcaaaaccga gcgtttgcaa ctgctcatcg
     7141 gcaatgattt gattgacgat agtggtaagc gtgttcgtcg cgtcaagcaa atgcagctgg
     7201 tagccttgcg cacgggcttc cacctcaacg aaatagcgcg aatctaccag aatgtgcgca
     7261 ctttcacggc taataaccac atagcctgat ccggtggaga tccccagatg cggctgtttg
     7321 ttctgccgtg aggagagaag cactgcatcc agttgttgcg ccttaagcca gtcgcgcagc
     7381 gaagcgagta atgtcatctg gattttcctt cttacaggct atcgattaac aatttacctt
     7441 tacggaacat catcagacgc aggaacacca ccatcagcgc agttatgact gcccccagcg
     7501 cgatccccgc catatagacg cccaggttag tgaccagcgg ccatgcccag atagcggatt
     7561 ccgggaacca ttgcactgcg cccagccaga cagcggcggt tgagccgaca atcgcgccga
     7621 ccatatacga cggaatggcg gtgatggggc tttccagcgc aaacggaatc gcaccctcac
     7681 taatccccat aaaggcaagg aacatcgcgg tcttaccctg cggataaagt tgagcgttga
     7741 acaggcgttt gccggttaaa cgacggtcga taatggtcgc cagacccaga cctatcggcg
     7801 gaataacgat agcgatagaa cgtgcagtga ccggtaatac gtggtcagtg gtaaagctga
     7861 aggcaacaaa acctgcggct ttgttgatcg ggccaccaag gtcgattgcg gtcgccgcgg
     7921 cgatccccat tgcgtacatc agcgcgccct tctcacctgc ggcagtcagc acggtacgga
     7981 taccgccgtt gatccagccg ccaaaggggg taatgacgta gtacatcgcc agcatgacaa
     8041 aaatggcgga aagaatcggt agcaaaaatg tggttttgaa cgctaacagg aaatccggta
     8101 actggatttt ctggttcatc cacttcacaa ggtaacccgc gacgatagaa ataatcagcg
     8161 caccaatgaa ggtggacggc accggcgaag aggtcgccca ttgcatagtg ctgggatcga
     8221 agttcagcag ttgcgtcggc tgggtagaca tcaagccgcc gataaaacca gccgggaacg
     8281 ccagtttgcc accgatagag ttcgccacaa aagcagcaaa catcggaatg gcaaaaccaa
     8341 acaacacgcc gccgaaggac tgcgacagcc aggcaaattt cagtaaggag aggtcgaaac
     8401 cggagaattt cccgctatta agagcatcca tgatgccgat ctcagcagga attttaagcc
     8461 agctataagc aatcagctgg ctgaaagcga ggatcacacc gcccataatt aaggtcggca
     8521 ccatgcgtga aatccctgac atcacatgct gcgggagttc accccagaag ctgcttttag
     8581 aggcctgcgg attcttaact gccgctgccg caccggatgc gccaggcaca acggttgcac
     8641 tgcgtttttt aatggccata acaattatcc ctgtcgaata ttactgttgt tcagaggcaa
     8701 tcatctcttc gatttctttg atgatgcccg cagcgttttt aattgcgtcc tgcaaagtga
     8761 tttcataaac gtcgcgtgat tcgaaacgct cattatcttc cggggtaact gccacggagt
     8821 ggatgatgat ggttgcttcg gcgatatcct gcgccgtcag gcgattctgg ataccgtccg
     8881 cgccctgggt ttcaattttc acttcataac ctgcttctac cgccgcttct tccagcgcct
     8941 gagcggccat aaaggtgtga gccaggccca ttgggcaggc acataaggca attagtttct
     9001 tactcatcgt taattcctcg ttcagtgata gttgacgagt gaaattatga acgtgcaaaa
     9061 caaatcgccg ttaccagaca aataatgcat ttactggaga attaatcccg ctgtacggaa
     9121 gtgtgacgca ggtcggcctt gtgaggggga cacaaagaaa aattgaatta actgtatgat
     9181 ttaaaagatt atcgggagag ttacctcccg atataacaga aaggatttac agaatgtgcc
     9241 ctaaggtctg gcgtaaatgt gcaccggaac cgagaagacc cggattgtca tggacgatga
     9301 gatacaccgg aatatcatgg acatattctt taaagcgccc tttatcttca aatgcggcac
     9361 ggaaaccaga ggctttgaag aactcaagga agcgcggcac gataccgcca gcaatataaa
     9421 ccccaccaaa ggttcccagc gttaatgcca gattgccgcc aaaacggccc ataatgacgc
     9481 aaaacagcga caacgcacgg cggcaatcgg tgcagctgtc agccagcgcg cgttcggtaa
     9541 tatcttttgg cttgagattt tctggcaggc ggttgtcggc tttaacaatt gcgcgataca
     9601 aattcaccag cccaggacca gaaagcacgc gttccgccga aacatgacca atttccgcac
     9661 gcaatatttc gaggataatg gcctcttctt cactattcgg cgcaaaatca acgtgaccgc
     9721 cttcgcccgg caagcttacc caacgcttat cgacatggac cagatgcgca accccaagcc
     9781 ctgttccggc accgtaaacc gcaataggtt taccttcgac cggttctgcg ccaccaaact
     9841 gaatcagatg ctcttttttc agcatcggga tcgccatcga tacagcggta aaatcgttaa
     9901 taatttccag atggctaaaa ccgagattct ttttcatttc ggcaattgag aacgcccagg
     9961 tatggttggt cattgccacc cagtcaccgg taattgggca agcgatggca atacagccgt
    10021 ctttcacctc gaccttatgt tcttcaagat aaacgcgaat gaccgcttcg aggctggggt
    10081 aatcaagccc tgaataggtc ttagcctgcg agatttcacc actggcaata tcacacagag
    10141 caagacgtgc gttggtgccg cccacatcac cgactaatgc atactttgtc attcttcaac
    10201 tgctccgcta aagtcaaaat aattctttct cacactgtaa atacctgggg gcataacaac
    10261 aacgccggaa aggcaggctc cctgtaaata tcgatctggg tcacacattt actttatcgt
    10321 ttcagcacca attgcagcga tgcctttttg caagctgggc aaactaagta tctgaccccg
    10381 cataaggaat agaacatgct ccatccgcga gccagaacca tgttgttatt atcgctcccc
    10441 gccgtggcaa ttgggattgc gtccagtctt attctgattg tggtgatgaa aatcgcctcg
    10501 gtattacaga atttgctctg gcaacgactg ccgggaactc tggggatagc ccaggattca
    10561 cctatctgga tcatcggtgt attaacgcta acgggtattg cggtggggtt ggttatccgt
    10621 ttcagccagg gtcatgccgg accagacccc gcctgtgaac cgctgatcgg cgcaccagtt
    10681 ccgccctctg cgctaccagg acttatcgta gcattaattc tcggtcttgc tggcggcgtc
    10741 agcctggggc cggaacatcc gatcatgacc gtcaatatcg cccttgccgt cgccattggc
    10801 gctcgtctgt taccgcgcgt caaccgaatg gagtggacta ttttagcctc tgccggaacc
    10861 atcggcgcgc tgtttggtac acctgttgcg gcggcgttga tattttcgca aaccttaaat
    10921 ggcagtagtg aagttccgct atgggatcgt ctttttgcgc cgttaatggc ggcagcagct
    10981 ggtgcactta ctaccggatt atttttccat ccccattttt cactgcccat tgcgcattac
    11041 ggacagatgg aaatgaccga tattctcagc ggtgcaattg tcgcggcgat tgccatcgca
    11101 gcagggatgg tcgccgtatg gtgcttacca cggttgcacg cgatgatgaa tcaaatgaaa
    11161 aatccggtgc tcgtgctggg tattggcgga tttattctcg gaattctggg ggttattggt
    11221 ggaccagttt cgctgtttaa agggctggat gagatgcagc agatggtggc aaatcaggct
    11281 ttcagcacca gcgattactt tttgctggcg gtaattaaac ttgccgccct ggtcgttgct
    11341 gccgccagtg gctttcgcgg tgggcgaatc ttcccggcag tgtttgtcgg cgtggcatta
    11401 gggttgatgc tgcatgagca cgttcccgcc gttccagcgg caataaccgt ttcctgcgct
    11461 attctcggca tcgtgctggt ggtaacacgc gatggctggt taagtctttt tatggcggca
    11521 gtcgttgtac ccaataccac attgctaccg ctgctctgta tcgtcatgct tccggcatgg
    11581 ctgttattag caggtaagcc gatgatgatg gtcaatcgtc cgaagcaaca gccaccccac
    11641 gataatgttt agcaaaaaat gctctccttt atgatttaag agttatggcg atgatacggg
    11701 gctttacctc cccgtaatat tgcgttaaca ggccgctgac ggcttcgatc gtgaaggaga
    11761 ataacaatgt tcaggtcact gtttctggcg gccgccctga tggcatttac cccgcttgca
    11821 gcaaatgcag gtgaaatcac cctactgcca tcgatcaaat tacaaattgg cgatcgcgat
    11881 cattacggta attactggga cggtggtcac tggcgagatc gtgattactg gcatcgcaat
    11941 tatgaatggc gcaaaaaccg ctggtggcgt catgataatg gctatcatcg tggctgggat
    12001 aagcgtaaag catatgagcg tggctatcgt gaaggctggc gcgatcgtga cgatcatcgc
    12061 ggaaaaggcc gcggacatgg gcaccgccat taaacgcgtc ctccaatgga gcacaatgcc
    12121 tgatgcgacg ctcattcaac tacaatccca gcgccgtccc caccaacaac cagatattca
    12181 gcgccacgac cagcactaca atcacccagc ctgtctgttt tacgcgtttg ctgttcacca
    12241 gatcgcccat caacttgctg tcactggtga aaatcagcag tggaaccagc gccagggcga
    12301 taccaaaact taacagcacc tgactcataa ccagaatccg tgtcggatct aatcccatca
    12361 gaatgacaat aaatgacggc aacatggtga ctgtacgacg cacccacagc gggatatgga
    12421 agcgaatgaa tccctgcatc accacctgcc ccgccagtgt ccccaccacc gttgaggaca
    12481 gtccggcagc aaccagactt aacccaaaga ccgttgccgc agcatggctt aacagcggtt
    12541 gcagcgtcag ataagcctca tcaagatcgg caacaccagt atgaccagaa aagtggaacg
    12601 ccgccgcagc tgtagccatc atcgccagat tgacaaaacc ggcaatcgtc atggcgatag
    12661 ccacatccca tttggtggcg gaataacgtt gttgacgcga accgccatgt aaatgctgag
    12721 tgagcgaaga gtgcaaataa atcacatgcg gcataatcgt cgcccctaac acgcctgctg
    12781 ccaggaagac cgcctccgaa gtaggtaaac tcgggatcac cattccttta cccagctgcg
    12841 ccaggttagg ctgggagaaa atcaactcga caatgtaagc cgcggcaaca aacaacagta
    12901 acccgccaat cactttctcc agtggttttt gcccgcgacg ttgcagcatt aaaatcagga
    12961 aagtcgcgat ccccgtcagc accgcgccct gcaacaacga gacaccaaga atgagtttaa
    13021 aaccgatcgc cgcaccaata aattccgcca ggtcggttgc catcgcaata atttctgcct
    13081 gaacccaata gaaccacact acgggacgcg gatagtgatc acgaatctgc tccgccagat
    13141 tttttccggt ggcaatccct agtttggcag agaggatctg aatcagcatc gccatcaggt
    13201 tggcccaaac gacaacccac agtagctgat agccgaagct ggcacccgcc tgaatattgg
    13261 tcgcaaagtt accgggatcg atataaccaa tcgccgcaat gaacgcaggt cccattaatg
    13321 cgagcctcat cttgcgcgcc gcccgtccgc tgctactctc aacgcgatag ttcgtcatct
    13381 tgtgcctcta aaacatagcc tttgctatgt ttcatgctat gataaacgag aatgattatc
    13441 aaattcattt aaatggattg tggtgatttc tctgatagac caggattatg actacgaaaa
    13501 gattgatgac ttgaatgtta tggttatgtt taatgttagc acatttacat aactttcagc
    13561 ttccatacac aacatagcag aaatgtatga cagatcacta tttttgaagc ctgtcacagg
    13621 acgtcattat agtgtgtgtc agatctcgtt ttccttaacc atgttacata gaatgtgcac
    13681 ggaaatttaa cctgcctcat atttggagca aatatggacc gcgtccttca ttttgtactg
    13741 gcacttgccg ttgttgcgat tctcgcactg ctggtaagca gcgaccgcaa aaaaattcgt
    13801 atccgttatg ttattcaact gcttgttatc gaagtgttac tggcgtggtt cttcctgaac
    13861 tccgacgttg gtttaggctt cgtgaaaggc ttctccgaaa tgttcgaaaa actgctcaga
    13921 tttgccaacg aagggactaa cttcgtcttt ggtagcatga atgatcaagg cctggcattc
    13981 ttcttcctga aagtgctgtg cccaatcgtc tttatctctg cactgatcgg tattctccag
    14041 cacattcgcg tgttgccggt gattatccgc gcaattggtt tcctgctctc caaagtcaac
    14101 ggtatgggca aactggaatc ctttaacgcc gtcagctccc tgattctggg tcagtctgaa
    14161 aactttattg cctataaaga tatcctcggc aaaatctccc gtaatcgtat gtacaccatg
    14221 gctgccacgg caatgtccac cgtgtcgatg tccatcgttg gtgcatacat gaccatgctg
    14281 gaaccgaaat acgtcgttgc tgcgctggta ctgaacatgt tcagcacctt tatcgtgctg
    14341 tcgctgatca atccttaccg tgttgatgcc agtgaagaaa acatccagat gtccaacctg
    14401 cacgaaggtc agagcttctt cgaaatgctg ggtgaataca ttctggcagg tttcaaagtt
    14461 gccattatcg ttgccgcgat gctgatcggc tttatcgccc tgatcgccgc gctgaacgca
    14521 ctgtttgcca ccgttactgg ctggtttggc tacagcatct ccttccaggg catcctgggc
    14581 tacatcttct atccgattgc atgggtgatg ggtgttcctt ccagtgaagc actgcaagtg
    14641 ggcagtatca tggcgaccaa actggtttcc aacgagttcg ttgcgatgat ggatctgcag
    14701 aaaattgctt ccacgctctc tccgcgtgct gaaggcatca tctctgtgtt cctggtttcc
    14761 ttcgctaact tctcttcaat cgggattatc gcaggtgcag ttaaaggcct gaatgaagag
    14821 caaggtaacg tggtttctcg cttcggtctg aaactggttt acggctctac cctggtgagt
    14881 gtgctgtctg cgtcaatcgc agcactggtg ctgtaaaacc atacataaaa aagccgggga
    14941 tcattccccg gcttttttac gcctgttaat cagctaatgg ctgcgggcga ccaatcaaat
    15001 acccttgcag atattgcacc ccgagcttat gcaatagcgc ctgctgctgt tgcgtctcga
    15061 caaactccgc gaccacactc aatgacttcg ctttcgccag atcggtaatt gatctcacaa
    15121 tcatcgcatc cagcgtattc gtgacaatat ctttcacaaa gacgccatca attttgatga
    15181 tatcagcctg caaacgcttt aaccgttcgt agttggcata tccggtgcca aagtcatcaa
    15241 tcgcaatccg gaaaccaaac ttatgcagct gctcgatgtt gtacatgctg ctttctgcgt
    15301 tagaaaacgc ctgctcctcg gtgatctcaa gaatgaccgc ctgcggggag atgtgataac
    15361 gcttaaacag acgaataatc cgcccggcaa tattcttttg cagcagcgtg agcggcatta
    15421 aattgactga aaagcgcggt ccttttttgt cgcaagggtg tgttgccagc cacttcaaca
    15481 aggattccag cacttgcaaa tcaaaacgcg cgctaaggtt aaactgagca ataaggggca
    15541 gaaacttatc cggggtcata atgccgccgt catatttcag tcgcgcgagg atctcatcat
    15601 aaccttcacc ctctttgttg cgaattggct gggcgtagag cagcaaatca ccctgatcta
    15661 acgccgtgcg aatggtattc agcaatagca cctgtttagt ggtctgcccg gaaaccatct
    15721 cctctctgct atccagcgcc agcacatgat gatgtgcgca ggattgctcc gccagccagc
    15781 ttaactgccc caacaagggt tgcagggttt cctgatttcc atcaaaacgc ccccaggcag
    15841 caccatagcc catatccagc ccggtattgt tccagtgaat ttgccgacta ttcaggatgt
    15901 taaccatatg ctggagtcgc ccttccgttt ccggcccgct cagcactaac agcagttcac
    15961 tacccggcaa ttgatataac ttttcgtttt cctgcatcaa cggcagcagc gtacggtaaa
    16021 ttgatcggat acagtgaacg cgcatcatta agccgtaatg acgactcata aactcaagat
    16081 tatcaatgcg caggcagcaa aactcttgcc cgcctcttgc tccggcgctt gttccaacgc
    16141 acggaagttg ggcaggagcg ttaacggatc ggtcagcgcc tgcaaatgcc agcggcgatt
    16201 aagccattca ctgcgatgat aaatgcgcac catatagagc aggcaaacgc taaaggaaat
    16261 cagcaccgca agaataaatg ccagcgaata ttcggtttcc accccttgca aaaagttctg
    16321 gttgtaattc agaaggcaaa gcgttgaaac agccctggtg agatttaaaa acggatagcg
    16381 aagcttaccg accccgaggg taaagatgat gaagaaaacg ggtaccaggt aaccggcaat
    16441 aaagtcgttt tcataaggtg tgcacaacag aagtagaagc acgcttaacg ctgccagcca
    16501 gcttaaggta aatgcgcgtt tctctttgcc caacgacggc gcgatatccc tgcgccacaa
    16561 tatctgcgca aagtggggac ttacaatcat gcgagtgaga tagtagaaaa gcatgttgta
    16621 aataagcaca gcggtgaaaa ggcttagcaa atcaacgacc gtgaaaatgg catccgcatc
    16681 gccgaaaaag gtggatatct ttagcgggaa atcaaagaag ctccccacaa gatacatgct
    16741 gcatttgatg ccgatcggcg tcaccaaacc aagccagaac agacgttgcc agacatatcg
    16801 gctggtcaat ccataacgcc aacgcgtccc cagctgccag cgtaatattg cgcaggcaca
    16861 caacacgaca aacgtctggc taccgagcat gaccgcagtc tgtaaaaaag agagatgaaa
    16921 gttccacaga ttggtgcaaa acatcccgca taaaacggga acgacaccgc gccagccaaa
    16981 gataaacaag attgacagca ttacgcacag tggcatccac gccagaaaga tataactgga
    17041 atggataatg gcaagtggcg aaataaaacg ggatagctga ataagtacca cggtgagcgt
    17101 aaacgctagt gtgaatatct tgatattttt tatcaggtta tgctccacaa acatgagtac
    17161 actactctca ctgatcgggg ttttcaggca cacaaataat aagtttattc gcatcgcgaa
    17221 gcaaacaggg ggccagcctg ttgccgattt ttcatccaat gatgctttca ccaggcggca
    17281 ctaacccgga cgtagactgt attacaaaag cggcaaaaag cagagacaaa aaacccccgc
    17341 tttgcagcga gggttggaaa tttggtggag ctaagcggga tcgaaccgct gacctcttgc
    17401 atgccatgca agcgctctcc cagctgagct atagccccac gatgcgttta cgtaccaaat
    17461 ttgctgggtg caaaatttgg tggagctaag cgggatcgaa ccgctgacct cttgcatgcc
    17521 atgcaagcgc tctcccagct gagctatagc cccgtcacgt aaagcttgtc gagttgacgg
    17581 gcggcatcat atgaattccg cccgaatgtg tcaacggcaa attgcaacgt gtagtttcaa
    17641 tcgctgaaaa atcaggcaaa tgaacaattt tggaaaaccg ctcgcattca gtagttattc
    17701 atgtcacggt ttcctgtaaa gtggtgttat aaaatgaact actaatagac ccacatacat
    17761 tcagggaatt gttatgttca aggagaggat gacaccagat gaacttgcca gactgaccgg
    17821 ctatagccgc cagaccatta ataaatgggt acgcaaggag ggctggacaa cgtcaccaaa
    17881 acctggcgtc cagggtggca aagcccgact ggttcacgtc aatgaacagg ttcgtgaata
    17941 tattcgcaat gctgaacgtc cagaaggtca gggagaagcg cctgcccttt ctggtgatgc
    18001 gccgcttgaa gtattgctgg tgacactggc gaaagagatg acgccagttg aacaaaaaca
    18061 gtttacatcc ttgcttctgc gggaagggat tatcggattg ttacaacgct tagggattcg
    18121 cgatagcaaa taatatgaaa agattacgca ataaaatgac caccgaagaa ctggctgaat
    18181 gcctcggtgt ggctaaacaa actgttaacc gttggatcag agaaaaaggc tggaaaacgg
    18241 aaaaatttcc cggcgtgaaa ggcggtcgtg ccagacttat tctggtcgat acgcaagttt
    18301 gcgagtttat tcagaacacg ccagccttcc ataacacacc aatgttgatg gaagctgaag
    18361 agagaattgc tgaatacgct cctggagcac gcgccccggc gtatcgacaa atcatcaacg
    18421 cgatagataa tatgacagac attgagcagg gaaaagtcgc gcaatttttg tcacgtgaag
    18481 gaattcgcaa tttcctcgcc cgtctcgata ttgacgaatc agcataaata aaaaacggca
    18541 ggatattatc tcctgccgtt tatcttttta cacgctaatt actgctgatt ttcgcgttca
    18601 gcaataaaat ccagcgcttt gttgatacgc tcgatactgc gggtcttacc aattgcgtga
    18661 acggtaacat ccagtgctgg agactgcccc gccccggtta cggcgacacg cagcggcata
    18721 ccaactttac ccatacccac ttccagctca tccgccgtcg cctgaatagc gtgatgaacg
    18781 ttttcagcgg tccagtcagt aatcgcggcc agtttgtcac gaaccacttc cagcggctga
    18841 cgcgctaccg gacgcagatg ttttttcgcc gcatcggcat cgaactcagc aaaatcttcg
    18901 tagaaataac ggcagctctg tgccatctct ttcagcgtct tgcagcgttc gcccagcagt
    18961 ttcaccagat cagccagctg cgggccgtta cgggtatcga tattttcctg ctcaatgtgc
    19021 cactgtaagt gagtagcaac atactccggc ggcagcgcgt taatgtagtg atggttcagc
    19081 cacagcagct tgtcggtgtt gaacgcactg gcagatttgc tgacggcatt caaagtgaag
    19141 tatttgatca tctcttcacg agtgaagatt tcctgatcgc cgtgggacca gcccagacgc
    19201 accagatagt tcagcagtgc ttctggcaaa taaccgtcat cacgatactg cattacgctg
    19261 actgccccgt gacgtttgga cagtttttta ccgtcatcgc cattgatcat agaaacgtgc
    19321 gcgtaaaccg gcaccggcgc tttcagggcc ttaagaatgt tgatctggcg cggcgtgttg
    19381 ttgatatggt cttcgccacg gataacgtgg gtgatttcca tatcccagtc atcgacaacc
    19441 acacagaagt tataggttgg ggaaccatcg gtacggcgga taataagatc gtccagttcc
    19501 tggttgctga actcgatcgg accacggatc tgatcgtcaa aaacaacaga accttcctgc
    19561 gggttagcaa aacgtacaac acacggttca tcatcagcat gatgctcatg gctgtggcgg
    19621 cagcgaccgt cataacgcgg cttctcacct ttcgccattt gctcttcgcg cagcgcttcc
    19681 aggcgctctt tagagcaata gcatttataa gcagtgccct cttccagcat ctgatcgatc
    19741 accgcgttgt agcgatcaaa acgtttggtc tggtagtacg gaccttcatc ccactccagg
    19801 ctcagccagt tcatgccatc cataatggct tcgatagctt ccggcgtgga acgctcaaga
    19861 tcggtgtctt caatacgcag cacgaactca ccgccgtggt tacgtgcaaa aagccaggag
    19921 taaagagcag tacgcgcgcc gccaacgtgc agatagcctg ttgggcttgg cgcgaagcga
    19981 gttttgattt tcatgaaatg gccttacgtt tagaaagatg ccgacaaccg gcaaatcctg
    20041 gaaaaattga gtgggcgata ttctatcact cccgcctgat tcctcaatgt agttcgggtt
    20101 tctaccttac gcattattgt tttcgttgac aaattgcgca acacggtctg ttttgcgatc
    20161 atcttgctta aatttacgac gaacgaacaa tttctttaga aaatgcgttg actcattttg
    20221 aactctccct ataatgcggc tccacacagc gggggtgatt agctcagctg ggagagcacc
    20281 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccaac tactttatgt
    20341 agtctccgcc gtgtagcaag aaattgagaa gtgggtgatt agctcagctg ggagagcacc
    20401 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccaat ttctcgccag
    20461 ctaaatttct tgta
//
LOCUS       DAZPAC010000057        20271 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZPAC010000057 DAZPAC010000000
VERSION     DAZPAC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20271
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.057"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(2..1651)
                     /gene="actP"
                     /locus_tag="ACHUPM_001785"
     CDS             complement(2..1651)
                     /gene="actP"
                     /locus_tag="ACHUPM_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR0869577.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1648..1961)
                     /locus_tag="ACHUPM_001786"
                     /pseudo
     CDS             complement(1648..1961)
                     /locus_tag="ACHUPM_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2161..4119)
                     /gene="acs"
                     /locus_tag="ACHUPM_001787"
     CDS             complement(2161..4119)
                     /gene="acs"
                     /locus_tag="ACHUPM_001787"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFR0869578.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4513..5949
                     /gene="nrfA"
                     /locus_tag="ACHUPM_001788"
     CDS             4513..5949
                     /gene="nrfA"
                     /locus_tag="ACHUPM_001788"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR0869579.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5994..6560
                     /gene="nrfB"
                     /locus_tag="ACHUPM_001789"
     CDS             5994..6560
                     /gene="nrfB"
                     /locus_tag="ACHUPM_001789"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR0869580.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6557..7228
                     /gene="nrfC"
                     /locus_tag="ACHUPM_001790"
     CDS             6557..7228
                     /gene="nrfC"
                     /locus_tag="ACHUPM_001790"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR0869581.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7225..8181
                     /gene="nrfD"
                     /locus_tag="ACHUPM_001791"
     CDS             7225..8181
                     /gene="nrfD"
                     /locus_tag="ACHUPM_001791"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR0869582.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8201..9919
                     /locus_tag="ACHUPM_001792"
     CDS             8201..9919
                     /locus_tag="ACHUPM_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR0869583.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSHSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9912..10295
                     /gene="nrfF"
                     /locus_tag="ACHUPM_001793"
     CDS             9912..10295
                     /gene="nrfF"
                     /locus_tag="ACHUPM_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR0869584.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10292..10888
                     /gene="nrfG"
                     /locus_tag="ACHUPM_001794"
     CDS             10292..10888
                     /gene="nrfG"
                     /locus_tag="ACHUPM_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR0869585.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11230..12543
                     /gene="gltP"
                     /locus_tag="ACHUPM_001795"
     CDS             11230..12543
                     /gene="gltP"
                     /locus_tag="ACHUPM_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR0869586.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12622..13311)
                     /gene="yjcO"
                     /locus_tag="ACHUPM_001796"
     CDS             complement(12622..13311)
                     /gene="yjcO"
                     /locus_tag="ACHUPM_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR0869587.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13405..15552)
                     /gene="fdhF"
                     /locus_tag="ACHUPM_001797"
     CDS             complement(13405..15552)
                     /gene="fdhF"
                     /locus_tag="ACHUPM_001797"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15133..15135),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFR0869588.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15750..17216)
                     /gene="mdtP"
                     /locus_tag="ACHUPM_001798"
     CDS             complement(15750..17216)
                     /gene="mdtP"
                     /locus_tag="ACHUPM_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR0869589.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17213..19264)
                     /gene="mdtO"
                     /locus_tag="ACHUPM_001799"
     CDS             complement(17213..19264)
                     /gene="mdtO"
                     /locus_tag="ACHUPM_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR0869590.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19264..>20271)
                     /gene="mdtN"
                     /locus_tag="ACHUPM_001800"
     CDS             complement(19264..>20271)
                     /gene="mdtN"
                     /locus_tag="ACHUPM_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR0869591.1"
                     /translation="PRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVV
                     PEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSVD
                     AQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADL
                     NAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSV
                     GQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSI
                     GYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEP
                     Q"
BASE COUNT         4520 a         5417 c         5565 g         4769 t
ORIGIN      
        1 attaatgtgc gcggccttgc tcaacgccaa agccggtctg ggagcggata aactgcgcgc
       61 ggaacagttc acgctcgcgc gccccttccg ctgagttatc ggttgccgag aagaaccaga
      121 tgccgaggaa tgccacggtg atagagaaca gcgccgggta ttcatacggg aagatggctt
      181 tttcgtgacc aaggatctgt acccaaatcg tcgggccgag gatcatcagt actactgcgg
      241 taatcagccc cagccagcca cccatcatcg cgccacgcgt ggtcagtttc gaccagtaca
      301 tagaaagcag aatgatcggg aagttacagc tcgccgcgat ggcaaacgcc agccccacca
      361 taaaggcgat gttctgattc tcaaacagca cgccgaggat aatcgcaatc acgccgagga
      421 tcagtacggt gatttttgat acccgcagct cttcacgttc ggtcgcgcct tttttgaaga
      481 cgttagcgta caagtcatgc gaaaccgcgg atgcgcccgc cagcgtcaga cccgcaacca
      541 ccgcgaggat agtggcgaaa gcaaccgctg aaataaaacc gaggaacagg ttgccgccca
      601 ctgcattcgc caggtgaacg gccgccatgt tgttaccacc aatcagatgg cccgccgcgt
      661 ctttatattc cggattcgca ccaaccagca tgatcgcgcc gaagccgata ataaaggtca
      721 gaatatagaa gtagcccata aacccggtgg cgtagaacac gctcttacgt gcttcgcggg
      781 catcgctgac tgtaaagaag cgcatcagaa tgtgcggcaa gcccgccgta ccaaacatca
      841 gtcccagacc cagagagagt gcggagatcg gatctttcac cagcccgccc ggcttcatga
      901 tgtcgacacc tttcgggtgt accgccatcg cttcactgaa cagattgttg aagctaaagc
      961 cgacgtgttt catcaccata aaggccataa agctggcacc gaacagcaac agcacggctt
     1021 tgataatctg tacccaagtg gtcgccagca tgccgccgaa caggacgtac atcatcatca
     1081 gcacgccgac cagcaccacc gcaatgtgat agttaaggcc aaacagcagc tcgatcagtt
     1141 tacctgcgcc caccatctgg gcgataaggt aaagcgccac caccaccaga gaaccacagg
     1201 ccgaaagaat acgaatcggc ccttgtttca gacggtaaga ggccacatcg gcaaaggtgt
     1261 agcgccccag gttacgcaga cgttcggcaa tcaggaacaa aatgatcggc cagcccacca
     1321 ggaagcccag cgagtaaatt aagccgtcat agccggaggt aaacaccagc gcggagatcc
     1381 ccaagaatga ggcggcggac atatagtccc cggcaatcgc cagcccgttc tggaagccag
     1441 tgatattgcc gcctgcggtg tagtagtcgc tacgagaacg tacgagtttt gatgcccagt
     1501 aggtaatgcc gagcgtaaac acgacgaaaa tcaggaacat aataatcgcc tgccagttcg
     1561 ttggctggcg ctctacggcc ccgctaatag catccgcggc gttagctgcg aaagggagtg
     1621 tggcggcaag cgccgtcaga actcttttca tgatgcttgt acctcatgca ggacttcatt
     1681 attaagacgg tcgaattcgc cgttcgcccg ccagatgtag ataccggtga gaacaaagga
     1741 gatcacaatc actccaacac caattggaat accgcgtgtg acgctggtgt tcggattcag
     1801 cggcgtgcca gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc
     1861 agcataataa tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa
     1921 tgcgcattgt cttctatccg ctgataaata gtgccattca tcacagattc tccagaggta
     1981 atgtagggat tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc
     2041 ttgccgaatg taggccggat aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg
     2101 acgctgtcgc gtcttatcag gcctacaaac cgttaccgac tcgcatccgg caattgtggg
     2161 ttacgatggc atcgcgatag cctgcttctc ttcaagcagc ttctcgacta cgccaggatc
     2221 ggcaagcgtc gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat
     2281 acggcgcata attttgccgg agcgggtttt aggcagggag tcggtccagt gcagcacgtc
     2341 tggcgtcgcc agcgggccaa tctctttacg cacccagttg cggacttctg cgtacagttc
     2401 tggtgacggt tcctccccgt gattaagcgt gacgtaggcg tagatcgcct gacctttaat
     2461 attgtgcgga atacctacta cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga
     2521 ctcaatctct gccgtcccca gacggtgacc ggagacgttc agcacgtcgt ccacacgccc
     2581 ggtgatccag taatagccat cttcatcgcg acgcgcgccg tcgccgctga aatacatatt
     2641 tttgaaggtg gagaagtagg tctgttcaaa acgttcgtga tcgccaaaca gcgtacgcgc
     2701 ctgacccggc caggagtcgg tgatcaccag gctgccttcg gtagcgcctt ccagcgggtt
     2761 accttcgtta tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc
     2821 tttcagctcg gtagcgccag gcagcggcgt gatcatgaaa ccgccagttt cggtctgcca
     2881 ccaggtatcg accaccggac atttctcgtt gccgattttt ttccagtacc actcccacgc
     2941 ttccgggtta attggctcgc ccacggaacc gagaatgcgc agcgacgaac ggtcggtacc
     3001 ctcgatcgct ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg cggtatagag
     3061 aatattgacc tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg gccagttggg
     3121 tacgccttca aacatcagcg tggtcgcacc gcaggccagc gggccgtaca gcaagtaact
     3181 gtgtccggtc acccagccca catcggcggt gcaccagtag atatcacccg gatgataatc
     3241 aaagacatat ttaaaggtca gcgccgcgta caccagataa ccgccggtag tatgcagcac
     3301 accttttggc ttaccggtag aaccggaggt gtagagaata aacagcggat cttcggcgtt
     3361 catcttttcc gcctggtgct gatcgctcgc ttgctcaacc aggtcgtgcc accacaggtc
     3421 gcgcccttcc tgccagtcaa ttttcccgcc agtacgcttc agtaccacca catgctctac
     3481 gctggtgacg ttcgggtttt tcagcgcgtc atcaacgttt ttcttcagcg gaatactgcg
     3541 cccggcacgc acaccttcgt cggaagtgat caccagtcgt gagttggaat caataatgcg
     3601 cccggcaacg gcttccggcg agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg
     3661 ggcgcaggcc agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa tcgccaccac
     3721 atcacctttt ttaatgccca gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg
     3781 cagctcttta tagctgatat gtttgctctg gctggcgtcg tcgccttccc agatgatggc
     3841 ggtacgatcg ccgttttctt gcagatggcg gtcaaggcag tttgccgcca gattcagcgt
     3901 gccgtcctcg taccatttaa tggacacatt accgggggca aaggaggtgt ttttcacctt
     3961 ctggtaaggt ttgatccagt caagaatttt tccctgttcg ccccagaagg tatcaggtac
     4021 gttaatagat tgttgataca tcgcctcgta ctgctgaggg tttatcaggc aacggtctgc
     4081 gatgttggca ggaatggtgt gtttgtgaat ttggctcatg cttttgttct ccttgtaggg
     4141 tgttaataat atgtggcata agcgttaaat gtaggggtat tggcagtttt gtttagtatt
     4201 tgggcgacag atcacgcaaa agtagaattg tgcaaataaa cggcagggta atttttgaag
     4261 gtcagcaaca aaagttgatt aattctttga ggaacatgca gttatgcatt gctgttgaaa
     4321 aagaggaaga tactaactaa ctctaaagtg gtattttaca tgcacttaca attgattaaa
     4381 gacaacattc acagtgtggt tatttgttac acataggggc gagcaatgtc atgacagtgt
     4441 aggtgcggtt actgtcgtga aaaacaataa aaaccgccat tgtaacaatg gcgcaattcg
     4501 gatgaagccc ctatgacaag gataaaaata aacgcacgcc gtatcttcag cttattgatt
     4561 ccttttttct ttttcacttc tgttcacgct gaacaaacgg cagctcccgc aaaacctgta
     4621 actgtggaag cgaagaatga aacctttgcc ccgcagcatc ccgatcaata tctctcctgg
     4681 aaagccacct cggaacagtc agagcgtgtt gacgccctgg cggaagatcc acggctggtg
     4741 atcctgtggg cggggtatcc cttctcgcgc gattacaaca agccgcgtgg acatgccttt
     4801 gctgtgaccg atgtgcgtga aaccctgcgt accggtgcgc cgaaaaacgc tgaagatggt
     4861 ccgctaccga tggcgtgctg gagttgtaaa agcccggatg tggcgcgtct gatccagaaa
     4921 gacggcgaag atggctactt ccacggtaag tgggcgcgcg gcggcccgga aatcgtcaac
     4981 aacttaggtt gtgccgactg ccataacacc gcctcaccag agttcgccaa aggcaaaccg
     5041 gagttaaccc tttcccgtcc gtatgcggct cgcgcgatgg aagccattgg taaacctttt
     5101 gagaaagccg gacgtttcga ccagcaatcg atggtttgcg gtcagtgcca tgtggagtat
     5161 tacttcgacg gcaaaaacaa agcggttaaa ttcccgtggg atgacggcat gaaagtcgaa
     5221 aatatggagc agtattacga caaaattgcc ttctctgact ggactaactc cctgtcgaaa
     5281 acgccaatgc tgaaagcgca gcacccggaa tatgaaacct ggacagcggg cattcacggt
     5341 aaaaacaacg tgacctgtat cgactgccat atgccaaaag tgcagaacgc cgaaggcaaa
     5401 ctctataccg accataaaat tggtaatccg tttgataact tcgcccagac ttgtgcgaac
     5461 tgccataccc aggacaaagc tgccttgcaa aaagtggtcg cggaacgtaa gcagtcgatt
     5521 aacgacctga aaatcaaggt tgaagatcaa ctggttcacg ctcacttcga agcgaaagca
     5581 gcgctggatg caggcgcgac ggaagctgaa atgaagccaa ttcaggacga tatccgtcat
     5641 gcccagtggc gctgggatct ggcgatcgct tcccacggca ttcatatgca cgcaccggaa
     5701 gaaggtttac ggatgctcgg tacggcgatg gataaagcgg cggatgcacg caccaaactg
     5761 gcgcgcctgc tggcgaccaa aggcatcacc catgaaatcc agatcccgga tatctcaacc
     5821 aaagagaaag cccagcaggc cattggcctg aacatggaac aaatcaaggc cgagaagcag
     5881 gacttcatca aaacggtgat cccgcagtgg gaagaacagg cacgtaaaaa cggtctgtta
     5941 agccaataac cccgttccgc ctcgcaaggg gcggaaaaca caatggagtg aatatgagcg
     6001 tattacgttc gttattaact gccggggtgc tggcgtcggg cctgttgtgg agcctgaacg
     6061 gaattaccgc tacccctgcg gcgcaggcat ctgatgatcg ctacgaagtt acccagcagc
     6121 gtaacccgga tgccgcctgt ctggactgtc ataaaccaga taccgaaggt atgcatggca
     6181 aacatgcttc cgttatcaac ccgaataaca aactgccggt cacctgcacc aactgtcacg
     6241 gccagccatc accgcaacac cgcgaagggg tgaaagatgt gatgcgcttt aacgagccga
     6301 tgtacaaggt tggagagcag aacagcgtct gtatgtcctg tcatctgccg gaacagttgc
     6361 aaaaagcgtt ctggccgcac gatgtccacg taaccaaagt ggcgtgcgcc agctgccatt
     6421 ccctgcatcc acagcaagat acgatgcaga cgttaagtga caaaggacgg attaagattt
     6481 gcgtcgattg ccacagcgat cagcgcacca atccgaactt taacccggcg tctgttccgt
     6541 tgcttaagga gcagccatga cctggtctcg tcgccagttt ctcaccggcg tcggcgtgct
     6601 ggcagccgtc agcggcaccg cagggcgcgt ggtggcgaaa acgttgaata tcaatggggt
     6661 gcgttacggc atggtgcatg acgagtcgtt atgcatcggc tgtaccgcct gtatggatgc
     6721 ttgtcgggaa gtgaacaagg tgccggaagg cgtctcgcgc ctgacgatta ttcgtagcga
     6781 gccgcagggc gaatttcctg atgtgaaata tcgcttcttc cgtaagtctt gccagcactg
     6841 cgatcatgcg ccgtgcgttg acgtctgccc gaccggtgcg tcttttcgtg atgccgctag
     6901 cgggattgtc gatgttaatc ctgatctctg cgtcggttgt cagtactgca tcgccgcctg
     6961 tccgtaccgc gtgcgcttta tccatccggt cacgaagacg gcggacaaat gcgatttctg
     7021 ccgtaaaacc aatttgcagg ccggtaagct gcccgcgtgt gttgaggctt gcccgacgaa
     7081 ggcgctgacg tttggcaatc tggacgatcc taacagtgag atttcgcaac tgctgcgcca
     7141 gaagcctact taccgctaca agctggcgct gggaaccaaa ccgaagctgt accgcgtacc
     7201 gtttaaatac ggggaggtga gccaatgacg cagacttccg catttcattt tgaatcgctg
     7261 gtgtgggact ggccgattgc catctacctg tttttgattg gtatttctgc cggtctggtg
     7321 acgctggccg tgctgttacg tcgcttctac ccgcaggcgg gcggtgcaga cagtacgttg
     7381 ctgcgcacca cgctgattgt cgggccgggc gcggtgatcc tcggtctgtt gatcctcgtc
     7441 ttccacctga caagaccgtg gaccttctgg aagctgatgt tccactacag ttttacctcg
     7501 gtgatgtcga tgggggtgat gctgtttcag ctctacatgg tggtgctggt gctgtggctg
     7561 gcgaaaatct ttgaacatga tttgcttgcc ctgcaacaac gctggttgcc gaagctgggg
     7621 atcgtgcaaa aggttctgag cctgctgacg cccgttcatc gcggactgga aacgctgatg
     7681 ctggtgttgg cggtgctgct gggggcttat accggctttc tgctgtcggc gctgaaatcg
     7741 tatccgttcc tcaataaccc gatcctgccg gtgctgttcc tcttctccgg catctcgtcc
     7801 ggtgcggcgg tggcgctgat cgccatggcg atacgccaac gcagtaaccc gcattccacg
     7861 gaagcgcact ttgttcaccg gatggaaatc cccgtggtat ggggtgaaat cttcctgctg
     7921 gtggcgtttt ttgtcggtct ggcgctgggc gatgacggta aagttcgtgc gctggtggcg
     7981 gcattaggtg gcggtttctg gacgtggtgg ttctggcttg gtgtcgccgg gctggggctg
     8041 attgtgccaa tgttgctcaa accgtgggtc aatcgcagtt ccggcattcc tgccgtgctg
     8101 gcggcgtgtg gggccagtct ggtcggcgtg ttgatgctgc gctttttcat tctctacgcc
     8161 ggacagttga cggtggcgta agccagaaaa gaggtggttt ctggacgtat tccttcctga
     8221 agtcggtttt ctggcgttgt tgttaagtct cggggtcaac gtgttgaccc cgttgacggc
     8281 ctttgcggga gtgcggttgc gctggcctgc catgatgcga ctcacttgca tcggcattct
     8341 ggcgcagttc gcgctcctgc tgctcgcctt tggcgtactg acgtattgtt ttctcatcag
     8401 cgatttctcg gtcatttatg tcgcgcaaca tagccatagc ctgctgtcgt gggaattcaa
     8461 gctggcagcg gtgtggggcg gccatgaagg ttcgctgctg ctttgggtgc tgctgctttc
     8521 cgcctggagc gcgctgtttg cctggcatta tcggcagcaa accgatccgc tatttccgct
     8581 gacgttagcc gttttatctc tcatgctcgc cgcactgcta ctgtttgtgg tgctgtggtc
     8641 cgatcccttc gtgcggatat ttccaccagc aatcgaaggc cgcgatctca atccgatgct
     8701 gcaacatccc ggtcttatct ttcatccacc gctgctttac cttggctatg gcggtttgat
     8761 ggtagcggcg agcgtggcgc tggcgagtct actgcgcggc gagtttgatg cggccagcgc
     8821 ccgaatttgc tggcgctggg cgttacctgg ctggagtgca ttaacggcgg ggatcatcct
     8881 cggttcctgg tgggcctact gcgaactcgg ctggggcggc tggtggttct gggatccggt
     8941 ggaaaatgcc tctttattac cctggctttc tgccactgcg ctgctgcaca gtttatccct
     9001 gacacgccag cgggggattt tttgccactg gtcgctgttg ctggcgatag ttactctgat
     9061 gctgtcgctg ctgggcacct taattgtccg ttctggcatt ctggtttcgg ttcatgcgtt
     9121 cgcactggat aacgtccgcg ccgtgccgtt gttcagcctg tttgcactga ttagccttgc
     9181 gtctttggct ctgtatggct ggcgagcgcg ggacggtggc ccggcggtgc gtttttcggg
     9241 gttatcgcgg gaaatgttaa tcctcgctac gctgttgctg ttttgcgcag tgctactgat
     9301 cgtgctggtg ggaacgcttt atccgatgat ttacggtctg ctgggctggg gacgcctctc
     9361 cgttggcgcg ccgtatttta accgcgcgac gttaccgttt ggtctgttga tgctggtggt
     9421 gattgtgctg gcgacgtttg tctctggcaa acgcgcgcag cttccggcgc tggtcgctca
     9481 tgcgggcgtg ctgttatttg ccgcagggat cgtggtttcc agcgtcagcc gtcaggagat
     9541 cagcctgaat ttacagccgg gtcagcaggt gacgctggca ggatacacct tccgttttga
     9601 gcgcctcgat ctgcaagcca aaggcaatta caccagcgaa aaagcgatag tggcgctgtt
     9661 tgaccatcag caacgcattg gtgaattaac gccggagcgg cgtttttatg aagcacgccg
     9721 tcagcaaatg atggaaccgt caattcgctg gaacggcatc catgactggt atgcggtcat
     9781 gggtgaaaaa accggagcgg atcgttacgc ttttcgtttg tatgtacaaa gcggtgtgcg
     9841 ctggatctgg ggtggaggat tgttgatgat tgcgggcgca ttgttaagcg gatggcgggg
     9901 gaagaagcgc gatgaataaa gggcttctca cgctactgct gttatttacc tgttttgcgc
     9961 gcgctcaggt cgtagacacc tggcaattcg ccaatccgca acaacagcaa caggcgttaa
    10021 atattgccag ccagttacgt tgtccgcagt gccagaatca aaacttactc gaatccaatg
    10081 cgccggtggc ggtcagtatg cgccatcagg tttatagcat ggtgtcggag gggaagaacg
    10141 aagtcgaaat cattggctgg atgaccgaac gctacggaga ttttgttcgc tataacccac
    10201 cgttaacggg tcagacgctg gtgttgtggg cgctgccagt ggtgttgtta ttgctgatgg
    10261 cactgatcct ctggcgagtg agggctaagc gatgaaacag cctcaaatac cggtgaaaat
    10321 gctgacaaca ctcacgatat tgatggtatt cctctgtgtc ggcagttatc tgttaagccc
    10381 aaaatggcag gcggtacgtg cggagtatca gcgtcagcgc gatccgctac atcagtttgc
    10441 cagccagcaa accccggaag cgcagcttca ggcattgcag gataaaatcc gcgctaatcc
    10501 acaaaacagc gaacaatggg cgttactggg cgagtactat ctgtggcaaa acgattacag
    10561 caattcgctg ctggcgtatc gtcaggcgtt gcaactacgt ggtgagaacg ctgaactgta
    10621 tgcggcgctg gcgacggtgc tttattacca ggccagccag catatgaccg cccagacttg
    10681 cgcaatgatc gacaaagccc tcgcgctgga ctctaacgaa atcaccgctc tgatgctgct
    10741 ggcttccgat gcgtttatgc aggcgaatta cgcgcaagcc atcgaactat ggcaaaaagt
    10801 gatggatctc aactcacccc ggatcaaccg gacacagctg gtcgagtcga ttaatatggc
    10861 aaaactgttg cagcggagat cggattaatc atctggtgat gaagattatg tcattgagct
    10921 gcataaaaaa taatcgaatg aacatatgcc aaaaataatc actaatcagt attattgcag
    10981 attaaacaaa taaaaatctt tccataacaa atggttattc attaatcctg ccataaactc
    11041 gcgttattat gcattaatgc agcgaaaagc tctgttgtta aagggttgcg caacataccg
    11101 cgcaaatgat actgatcata agcgttaaaa aaatctacaa accaacgcaa cacaattcat
    11161 gccctggcag tatgtcacgt tctcgcgttt ctgaacgggg aacggcgctc cattgaggaa
    11221 gttattctga tgaaaaatat aaaattcagc ctggcctggc agattctgtt tgctatggtg
    11281 ctgggcattc tcctgggaag ctacctgcac taccatagcg acagccgcga ctggctggtc
    11341 gtcaatttgc tctctccggc gggtgatatc ttcatccatc tgatcaaaat gattgttgtg
    11401 ccgattgtga tctccacgct ggtggtgggt atcgcgggtg ttggtgatgc caaacagctc
    11461 gggcgtattg gcgcgaaaac cattatttac ttcgaggtga tcaccaccgt cgccatcatt
    11521 ttggggatca ctctggcgaa cgtcttccag cccggtgccg gggtggatat gtcgcagctg
    11581 gcgaccgtcg atatctcgaa atatcagagc actacggaag cggtacaaag cagttcccac
    11641 ggcattatgg gcacgatttt gtcgctggtg ccgacgaaca ttgtggcgtc gatggcgaaa
    11701 ggcgaaatgc tgccgatcat ctttttctcg gtgctgtttg gtctggggct ttcttccctg
    11761 cccgcgacgc atcgtgaacc gctggtgacc gtgttccgct ccatctctga aaccatgttt
    11821 aaagtgactc acatggtgat gcgttatgca ccggtgggtg tgtttgcgct gattgcggtg
    11881 acggtggcta actttggttt ctcttctctg tggccactgg cgaaactggt gctgctggtg
    11941 catttcgcca ttctgttctt cgcgctggta gtgctgggaa ttgtggcgcg cctgtgcggg
    12001 ttaagcgtct ggatcctgat tcgtattctg aaagatgagc tgattctggc gtactccact
    12061 gccagctctg aaagcgtact gccgcgaatt attgagaaga tggaagccta cggagcaccg
    12121 gcgtcgatca ccagcttcgt ggtgccgacc ggttactctt ttaaccttga tggttcgacg
    12181 ctgtatcaaa gtattgccgc tattttcatc gctcagctgt atggcattga cctgtccatc
    12241 tggcaggaaa tcattctggt gctgacgctg atggtgacct cgaaagggat tgctggcgtg
    12301 ccgggcgtgt cgtttgtggt gctgctggca acgctgggta gcgtaggtat tccgctggaa
    12361 ggtctggcgt ttattgctgg tgttgaccgt atcctcgaca tggcgcgtac tgcgctgaac
    12421 gtggtgggta atgcgctggc ggtgctggtg attgccaagt gggaacacaa atttgaccgc
    12481 aaaaaggcgc tggcttatga gcgtgaagtg ctgggcaaat ttgataaaac tgcggatcaa
    12541 taattgaaga ttgccgggga tatccacccg gcaatgtgtg aatgcctgat gcgacgcttg
    12601 ccgcgtctta tcaggcctac cttaaccgtt ggttaatttt tcaaactctt cacacccggt
    12661 atcaaaccct tccatacagc tcaggttcaa ccagtgcagc gccttttgct tgttcttctc
    12721 gataaaacct tcttcaccgt ttaagaacat cattcccgcc cagtactcgg aataaccggt
    12781 tcgggaaatt gcagagctgc gtttgaaata ccaggttgcc ttgtcatcgt cggccttaat
    12841 gcccacgccg ttggcgtaaa tcaaaccaag cagcatttgg gcatcgacgg cagagtcgtt
    12901 ctccagatct tccgaggcgt tttctaacag cgaaatagcc tttggataat ccggtttacc
    12961 cgcttgagta tttaccagaa tatgcgccag cgttacttca ccttctttac tgcccgcttg
    13021 cgccgctttc tctgcaagaa cttttgcttg tggatagtcc agactgaccg gattggtgat
    13081 tttgatctgc gccagcagcg cgcaggcatc agcatcaccg ctgtcggcgg ctttctgtgc
    13141 ccaatattcg gctttgctca aatcgccgga gctaaaccag ctgtcggcaa gaaaatattg
    13201 tgcgcgtcgg tccccggcct ctgctgcctt taaatactgg ctgccaggct cggagtcgtt
    13261 ggcgtgggca aagaatgtca aaaacaacat caatgcgata atttttttca ttttgataat
    13321 acgattgggt agggagtaac cagtataaag agttcgaatg gataaaaaaa cagcctccga
    13381 aaggaggctg tagaaaggac tgtattacgc cagtgccgct tcgcgcaggc gagttttcaa
    13441 cttgttgtac tcgtcaatca cgtactgctc ggcggcgcgc tgatcggcga tcggctcgac
    13501 gcgcacggcg cagtatttgt attccggcgt tttcgtaatc gggcttaagt tttcggtaac
    13561 cagctcgtta caggcaccaa tccaccactg gtaggtcatg taaatcgccc ctttgttcgg
    13621 acgatcgctg acctgcgcac gggtgataat tttgccttta cgcgagtgca cccaaaccaa
    13681 tgcctcatct tcaataccca gacgtttggc gtcttcggta ttgatttgtg cgtagccagg
    13741 ttcatcagcc agcgccgcca gtgccgcaca gttaccggtc atcgaacggc aagagtagtg
    13801 accaacttca cgcaccgttg acagtaccat cgggtactcg tcggtgagtt tgtcgattgg
    13861 cgctacccag tcgcaggtga agaactgcgc cagaccgttc ggggtatcaa acttctcttt
    13921 aaacagataa gaagtcccct gatcggcatc tgaagtatcg cggcaaggcc actgaatgaa
    13981 gcccagttcg cccattttct cgtaagtcgc accgtagaaa tccgggcaca gatgacgcaa
    14041 ctcatcccag atctcctggg tgttgttgta gtgcatcgga taacccatac gggtggcgat
    14101 ttcactgatg atttgccagt ccgttttcag atcccatttc ggttcaactg ctttgaagaa
    14161 acgctggaag ccacggtcag ccgcagtaaa cacgccttca tgctcgcccc acgacgttga
    14221 cggcaaaatg acatccgccg ccgacgcggt tttggtcata aagatgtcct gaacgataac
    14281 cagttccaga tcttcaaagg ctttacgtac tgccgacagc tccgcgtcag tttgcagcgg
    14341 atcttcgccc ataatgtacg cggcacgcac ttcgccatgc gctgcgcggt gcggcagctc
    14401 gctgatgcga taaccggtat gagcaggcag gctttccacg ccccaggctt tggcgaattt
    14461 ctcgcggtta gccggatctt tcacgtactg gtatcccgga tacgtatccg gcagcgcgcc
    14521 catatcgcag gcaccctgca cgttgttctg accacgaacc gggtttacac ccgcatgcgg
    14581 cttaccgagg ttaccagtca gcatcgcgag gctggtcaga gaacgcacgg tttccacgcc
    14641 ctggtagaac tgggttacac ccatgcccca caggatggcg gcgcttttcg cctgggcata
    14701 catccgtgcc gcctgacgaa tctcactggc gctgacgccg gtgatatctt caaccgactc
    14761 cggcgtgtag ccttcaacga ttttacgata ctcttcaaag ccttctgtac gggaagcgac
    14821 gaacgctttg tcgtacagat tttcttcaat aatgacatgg cccatcgcat tcaacagcgc
    14881 gatgttcgag ccgtttttca gtgcaatgtg catgtcagca atgcgcgcgg tttcaatttt
    14941 gcgcggatcg cagacgataa ttttcgcccc gttacgttta gcgttaatta cgtgattcgc
    15001 cacgattggg tgggaatcag ccgggttgta cccgaaaacg aacactaaat cggtattatc
    15061 aatttcgtta atagcattgc tcattgcgcc attaccgacc gattggtgca gacctgcaac
    15121 cgatgggccg tgtcagacac gagcgcagca gtcaacgtta ttggtaccaa taacggcgcg
    15181 cgcaaatttt tgcattacat agttggtttc gttacccgta ccacgcgagg agccggtcgt
    15241 ctggatggca tccggaccgt acttctcttt gatggcgctc aggcgctcgg taacgtaatt
    15301 cagtgcctca tcccaggaaa caggttcgag tttgccgcca cgctggcgac ggatcatggg
    15361 ggttttcagg cgcggggtca ggatctgggt atcgttaatg aaatcccagc cataataacc
    15421 cttcagacac agggtaccct ggttggtttt cccctgcgct gcctccgccc ggacgatttt
    15481 gccgttatcg acgaccaggt tgattttgca acctgatgcg caataggggc aaaccgtgac
    15541 gacttttttc atcggtctcg ctccagttaa tcaaatcacg catacgcgct ctcgactaca
    15601 gtatgcatct tttatgccac attttatttg gggtcattcc ctgatattac gggcactatt
    15661 tattcaaaac tctgacgaaa aacaggctgt cgtcagtttt gacgtgacga aacgaaatac
    15721 cgcgtgacag ccatcacgcg gcagacattt tattttttct cgacgacggg agccgcctga
    15781 tacccgccgc ccagcgattt catcaactga atgctttgga tcacccggcg gctgtccagc
    15841 atcagtaatg acatctcttc ggcaagtact ggcaaccggg cttcagtggc ctgtaagcgg
    15901 ctggttaagc cgcgctgata ggcggcctcg gcagcgcgct gggtgaagcg cgttgcttcc
    15961 acgcgttcag cctgcatttc tcgctcgtcg ttgagcgttt gcagacgcgt gccgttgacg
    16021 gcaacgtcac gcaccgcgtt cagtactgac tggttgtaac gttcaatcat catgttgctg
    16081 gcggcgcgcg tgccttcgag attggcattc aaccgtccac cgtcaaacag cggcaatttc
    16141 agacccggga tgaagttgaa ctggcgactg gtttttttga ataaggtatc caggtggatg
    16201 gcatcaaggc cgaaaaacgc tttgatatca aagctcgggt agaacagcgc ccgcgcggaa
    16261 tccacctgat ctaatgacgc ctgaacatac cagcgcatgg cttgcagatc cgggcgtctg
    16321 gcgagcaact cataagagag tgtcgccgga atgccggtct ggactcgtgg taatgccacc
    16381 ggtttgatct ccggcatatc gctggccccc gcgccaatca atgcacgcag agattctcgc
    16441 gtttcggtga tttgcccttt gacggcagca atttgtttat cgaccgccag aatctgtgcc
    16501 cgcgcgccgt ggaaaggcac ttgtgcttcc agaccgtgcg ccactttact ctggtgcgct
    16561 ttcaccgcgt aatcaatcac atcgcgagtt tgttctaaca gatcgagcat ctgatagctg
    16621 gcctgcatac tgtaataaag ctgcgctacg cccgtggtca gcgatagctc tactgctgcg
    16681 gtttctgcca gcgcggcatt atgcgcgcca atggcggcgg caaccgctga gcgatgcaca
    16741 ccccacaaat caagatccag tccggcaaac aaacctactg tggcttccgt atagtacggc
    16801 ccgtccatac ccagtgcggg cgcatccatc gcataagggc ttaaaaagcc gttcgccgag
    16861 acgcgttggc ggttgagcat ccctaacgct gcgacctgta attgtgaacc ggcatctaac
    16921 aaatcggcct gggactgcgc tttttcttcc cgcagtttcg cttcggcgag ggtgtgtgaa
    16981 ccacttagcg tccgttggat cagcgcatcc agctgcgggt cattgagttg tttccaccac
    17041 tgcgcctgcg gccagccgga gctggcaaga tgaatatcat cggccagttt gatttgttcc
    17101 ggtttgagct gttgatgagg cgcagaatcc ttgcgtacca aggcacagcc ggaaatcagc
    17161 gtcgtgctgc cgagaatgct gcacaacagc agacgtgaaa gttgacgatt gatcattgcg
    17221 tggctccttg tgcctgttcc gtggcgggcg ttaatgccgg ggctgtccag tctggcaagc
    17281 gggcgaaaag ctggcagacg tgttgctcct gttttaataa ggtgggcagg atggcctgcg
    17341 aggcgggtgt ctcttcaagc gttatttgag gagaacggct gagtgcggtt gccagaccgg
    17401 cagcgtattt ttccagggcg tcggcaaact gacctgctct gtccggctgt agtgcgttat
    17461 ccagcgcctg cgccgaattc caggtggcat cccaggcgtg aaggatatcg cggccctgac
    17521 gaataaccgt ttgcgaacgt tcaatcagta aggcgcgttc ttcgctgtcc agttgacgct
    17581 ccagcgccac gcgttggcac atctcttcac aggcattaaa cgccgcatgc agaccgatac
    17641 gaatttgcag ataagtgcgc agagccgtga cttcctgctg gcgtgggatc cgcattactt
    17701 tacttaacat acccagcgcg ccagccagtt tttgcggcag cgtgcgcgct tcactttcag
    17761 gccagacaaa ggtgtaaatc accgcggaca ccacggtacc aatgatgata cccagggcgc
    17821 gatcgcgaat ttccaccagg tcgtacactg ggccaaaaac gttttcgagc gtggcgagcg
    17881 cgaaggtgac caccatctgt gtgccgatat aagaagagcg ttcagagctg gtggcaatcc
    17941 atgcgcccaa caggaaaatc ggtgccagca caaacagcaa ttcgacaata ttgtccagcc
    18001 agggcatgac cagtagcgtg aataacagcg ccagaatcgc gccgcaaaag gccccgccaa
    18061 aacgcagcac catcttctgg tacgacgaac cgacatttgg attagagacg atcacgcagg
    18121 tcagcataca ggtgtgaatg ccttcccagt ccacgccgct gtaaaaggtg taacagatca
    18181 aacaggcgag caatgttttt accgcgtagc gtatatagtc tggattggta aaagcatcgg
    18241 cgaccattga tggcggtttg gcggcgggcg ttggcggcgt attcgggttc atctggccca
    18301 gttgtaacaa cgtctggcag atattctcca ggttacattc ccgtgccgcc accgcttcac
    18361 tttcactgat ccgccagtcg ctttgccagc actgaccttc agcaacggca tgctgcagct
    18421 tgttgatttc tgaggctaat ttttgccgaa attcaataat tgcctgagaa tcagcaaaag
    18481 aggtgggatc gtagcgattc agcgtcgagt aaatgtaggt taccgttgcc acgcagcttt
    18541 gccaccatgc gctttgagtt cgccagttgg catcgtccgc gagacaaaag acattcagtt
    18601 tttgcagcgc cagcgcctct ctttcaatcc gcgtttcggg tagcggtgcg aggctgtctg
    18661 tcaggtgact aatggcatca tcaagccgat cattaagcgc ctgatgcatt tgcgaaatgg
    18721 cacgactggg aaaccacagc acgccgatta acgtcatcag caaggttgga tagaggccaa
    18781 caacgataca ccacagcgtt aagcgcacga ccacttccgg atagtcgagc atggcgggga
    18841 aggtttgccc gtaaatggcg acaatggcga cagcgaaaaa gaccagtccc agccgatggg
    18901 tgcgcatcaa aaacatgcgg cccatcagga tcggcccggc gatgatcaat cggatcaacg
    18961 gttcgccgta tgaccatttg tagatcaaaa acaggctgcc gatctccagc accgtggcaa
    19021 ccacaaacaa gatcgccaca aatttggtgt aaaacgcgtt cgactgaata ccgtaaaaca
    19081 gcactgccag cgataacgcc acaaaaggga tctcaaaagt catcgagatc agaatcacca
    19141 gcaggcagcc tacccagagt tgcacgatct gcggcacgcg gccgggtcgc cgctcgctta
    19201 actcttcatg aaagaacgcc agcagcctga ccatcggtaa tggcagggag ttgagcgcgc
    19261 tcattattgc ggctcaagat tagcgactgc cgaagcgccg atgcggaaca tttccgggtc
    19321 aggtttatcg accatgattt tgaccggaaa acgctgggca acgcggaccc agttaataga
    19381 gcgagaaact ttcggcaggc cgcccagcac caggccgccg tcatccggta gcacgccgta
    19441 gccaatcgaa tccactttac cctcgaaggt tttgccgctg tcactcatca ggcgaatcgt
    19501 tgcgggtgta cctgagcgaa tatttttcag atcggtttcg cggaagttgg cgatcacgta
    19561 ccagtgacga gtgtcgatta gggtaaaaat agggcgcatg gcagaagcaa attgcccgac
    19621 ggaggttttg agggaaatga cccggccatc aaacggcgcg cgaacggtcg ccatttccag
    19681 atgcagtttg gtcagggcaa tatccgcttc gaccgccgca cgttgcgcca ccagcgcatc
    19741 gacgccgctg acggcgctgg cggctgactg cgcctgtaac aatacggcat taagatcggc
    19801 ttctgcggcg cgctgcgcag ttcttgcacg gtcaacgtct tccgctgaga caaaaccctc
    19861 tttcagtaat ggctcggtac ggcgtaatgt atctgtggcc tgtttcgcgg cggcacgggc
    19921 tttttctacc gtggcattaa tcgagtcggc accaaactgt tgcgcatcaa cgctacgctg
    19981 ggtgagcata atttgcttat ccagcgccgc gagggaggct tcagctttcg ccagattggc
    20041 ttcgtacggg cgcgggtcaa tgcggaacag caaatcgccc tgtttgactg cctggttgtc
    20101 ggtgaccgcc agttctacaa tgcggccgct gacttccggc accacatcaa tggtatctgc
    20161 tgacgcgtaa gcgtcattag ttgatggcgc actgtctacg cgccagataa cgaaaacaag
    20221 ggcaaccagc gccaatgcaa ccactaacag agcagggaat ttactgcgag g
//
LOCUS       DAZPAC010000058        20147 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZPAC010000058 DAZPAC010000000
VERSION     DAZPAC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.058"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="ACHUPM_001801"
     CDS             complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="ACHUPM_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFR0869592.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQ"
     gene            complement(2109..2687)
                     /gene="mocA"
                     /locus_tag="ACHUPM_001802"
     CDS             complement(2109..2687)
                     /gene="mocA"
                     /locus_tag="ACHUPM_001802"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFR0869593.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            2790..3560
                     /gene="yqeC"
                     /locus_tag="ACHUPM_001803"
     CDS             2790..3560
                     /gene="yqeC"
                     /locus_tag="ACHUPM_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFR0869594.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            3608..5233
                     /gene="yqeB"
                     /locus_tag="ACHUPM_001804"
     CDS             3608..5233
                     /gene="yqeB"
                     /locus_tag="ACHUPM_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFR0869595.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(5274..6206)
                     /gene="arcC"
                     /locus_tag="ACHUPM_001805"
     CDS             complement(5274..6206)
                     /gene="arcC"
                     /locus_tag="ACHUPM_001805"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR0869596.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFVRSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(6254..7638)
                     /gene="hyuA"
                     /locus_tag="ACHUPM_001806"
                     /pseudo
     CDS             complement(6254..7638)
                     /gene="hyuA"
                     /locus_tag="ACHUPM_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            complement(7691..8902)
                     /locus_tag="ACHUPM_001807"
     CDS             complement(7691..8902)
                     /locus_tag="ACHUPM_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFR0869597.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(8960..10156)
                     /gene="dpaL"
                     /locus_tag="ACHUPM_001808"
     CDS             complement(8960..10156)
                     /gene="dpaL"
                     /locus_tag="ACHUPM_001808"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFR0869598.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(10214..11401)
                     /gene="ygeW"
                     /locus_tag="ACHUPM_001809"
     CDS             complement(10214..11401)
                     /gene="ygeW"
                     /locus_tag="ACHUPM_001809"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFR0869599.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            11880..13658
                     /locus_tag="ACHUPM_001810"
     CDS             11880..13658
                     /locus_tag="ACHUPM_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFR0869600.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(13698..14177)
                     /gene="xdhC"
                     /locus_tag="ACHUPM_001811"
     CDS             complement(13698..14177)
                     /gene="xdhC"
                     /locus_tag="ACHUPM_001811"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR0869601.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(14174..15052)
                     /gene="xdhB"
                     /locus_tag="ACHUPM_001812"
     CDS             complement(14174..15052)
                     /gene="xdhB"
                     /locus_tag="ACHUPM_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR0869602.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(15063..17360)
                     /gene="xdhA"
                     /locus_tag="ACHUPM_001813"
     CDS             complement(15063..17360)
                     /gene="xdhA"
                     /locus_tag="ACHUPM_001813"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR0869603.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            17775..18530
                     /gene="actS"
                     /locus_tag="ACHUPM_001814"
     CDS             17775..18530
                     /gene="actS"
                     /locus_tag="ACHUPM_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR0869604.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            18609..18678
                     /locus_tag="ACHUPM_001815"
     tRNA            18609..18678
                     /locus_tag="ACHUPM_001815"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18641..18643,aa:Gly,seq:ccc)
     gene            18883..>20147
                     /locus_tag="ACHUPM_001816"
     CDS             18883..>20147
                     /locus_tag="ACHUPM_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFR0869605.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTLD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT         4980 a         4765 c         5179 g         5223 t
ORIGIN      
        1 aactgaggaa tgatattttt caccacgccg ccagcattgg cttcgcgttc aaacagcgta
       61 accggatggc ccgctctggc aaggaagtaa cctgctgcca gacccgccgg acctgcacca
      121 atcacggcaa ccggatggcg tgaaccagaa ccggcgggtt tgtgccagcg ttgcttatat
      181 tcatcccaac ctttttccag cgcgactttt ttcagttcgc ggatattcag cgcactgtcg
      241 taatccaggc gggtacagtt gtattggcac tggtgatcgc aaatatgacc ggtaatggca
      301 ggtagggcgt tgcgttggta gatgagttcc agcgcgtcgg catagcggtg ttcgccaagc
      361 agacggatgt attccggaat atcttgcttg atagcgcagg cagtaacaca gggggcaacg
      421 tagcaatcgg tcagcggcag gtcttctgcc acttcaatac gctcttctgg cttccagtgt
      481 ttctgggtgt attccatggt taacgcgtct gctgccagtc tgttcagtcg ttcgacgtca
      541 acatggtcaa gtccccaggc gtcggagcct tccagctcgc gcatgcaggc acttaagcgc
      601 agatagccgc caggtttcag caggtcggtt gccatagtaa tagggcgaat acctgtatca
      661 aaaatatcgc ggatagtcag ctgactggca ccaccggaat aagaaatggg cagtttgccg
      721 tcaaaggcgc gagagagaac tgctgcaaca ttgatggaga gcgggaacag cgcacggcct
      781 gacatataca tctcttcacc aggcagtgcg cctttattgt tgatggtgcc gagagtgtta
      841 gtcagtttta cgccaaagcc gagtgatttt tcttttgcca gtaccatcag gcgctccagc
      901 atttccagtg cttgcgtcag cttgaggtcg tgatcaaatg actcttcttt taagcctatg
      961 taaccgaaac cgcagacatc gaggatctca cgaacacgcg cgtaccccag taaggtcggg
     1021 ttaagtttga caaaggtgtt gagccctttt tcttccagca tgtagcggca aatggcttcg
     1081 atttcatgcg gaggacagcc gtgcatggtg gagaggggga cgccctgcac catactggtg
     1141 gggatgcgag cgggtaaggc ttgcaagttt tcgcgttttt cctgcaatcc gtggcgggct
     1201 aaaaatgcgt catcctggag taatttattc agcgtatcgc gatattgagc gaatttcgga
     1261 tggtcagatg cgtccatcat attgtcgatg aactgttgca tcggcggttg cttaatacct
     1321 tcgaggttgt aaccgatgct catattaaag atgaacgatt taccggaatc agaaggctgt
     1381 aacatcgctt cgagaaggtg cagggcaaac caggctttga ggtattcatc ccaggcttta
     1441 agcaaggtaa attcggtaga ccattcggtg ttaaagcatt cgtcttcggc atcaatacag
     1501 ggcttttcca gctccaggcg gtcaagaatt tggacggttt ttagttcgat gaagcgtccg
     1561 ccagtcagcc aggaagtgac aatattttgc gcgagctgcg tgtgcgggcc agcggcaggg
     1621 ccgacgggag tggcacaggt ttcaccgaag acgctaacag ttttaccttt tacaggtgag
     1681 taaaactgtt gctcgggaat accaaagatt gagcgttgtt gttggtattc atcaaatatg
     1741 cgcgtcaaaa gttcctcaaa cggaatggga cgcataatat cccccataac cctctcctta
     1801 gcttatacag atatcattga gtgaccggtg ttgagaaatc cggtggttta gggggataga
     1861 gcaacaatcg caccactctt gccaggtttg gcgggaaata taaaaaaatg tgaggtggct
     1921 cgaaacttaa aggctttttg tgaatgaact cacaggtgcc aaatgagaat taaaatcact
     1981 cctgaaaatt attattgtca gcccatttat cactttttct cttttctcaa aatcagtgat
     2041 tccttatcat tatgataaag agtgctggag agattaaata tttgaacgca atcgtttaac
     2101 tacattttct aaattttagt ataccttttt tgcgctgtaa taaagtcgtc cggagtatca
     2161 atatctaaaa ttatttctgc attttctatt tcgacggaat aatgatctcc cataaggagt
     2221 gcctgacgca tattggtgac attgggtcgc tggattgcct gcatcaggca tggttttgac
     2281 actaaaatcg gatggccggg gatgccatta tggagtggca gtattgcgcc atcatttcgt
     2341 aacgaccaga tttttctaaa aatatcgatg gtgagggttg gcatgtcacc gtgggtgaga
     2401 aaacaatgtt ctgtttgcac tgcgggtact gcggccttga cggatgttag taaaccctgc
     2461 gcataatctg ggttgtgaat aatagtgata ttgctctggt tcgcataacg ttcgtgcagt
     2521 tcattaccac gatagccggt gactaaaata attcggctac aaaactgcaa cgcatttttg
     2581 atacttgtat caagaattgt tccctgttcc caggggaaca tcattttcca ttgccccatt
     2641 cttgatgata atccggcagc ggtaattata cagtcgatgg ctgacatagt ggttccatta
     2701 tttatagggg ctaataacgc ctgataattg cgttacttcc tgatggatta agttggggat
     2761 aatttatccc agagaggtca taaagactcg tgaaaagtat agttgaccca tcggcattag
     2821 tcattgattt aggtgcgcag aaacgtccca ctgtgatttc tgttgttggt gctgggggga
     2881 aaaccagctt gcttttttgg ctggcagaac tgctccaggc tagcggcagg cgtgtgttaa
     2941 tcacgacgac gacacatatg tttatgccaa cgtctcactg gcccgtggtt ttctgtcgtg
     3001 atcccgccat gcttcctcat gcgtctctta catcccccat ttcattttgt tttcacagct
     3061 ggaaagcgaa ccagggaaaa gtgcagggat ttacgccaga agcgattgat gcactggtgc
     3121 aacgaccaga gtgtgacgta attctcattg aagcagatgg ctcgcgtgga atgccgttaa
     3181 aagcgcctga tgagcacgaa ccttgcatac ccaaaagcag ttgctgcgtg attgctgtga
     3241 tgggaggaca tactttgggc gcgaaagtga gcacagaaaa tgtccatcgc tggtcgcagt
     3301 ttgctgatat taccgggtta acacctgatg caaccttgca actgagcgat ctcgttgcgc
     3361 tggttcgcca ccctcagggg gcgtttaaaa acgtaccgca aggttgtcgg cgagtctggt
     3421 tcattaaccg tttttctcaa tgtgagaatg cgattgcaca aagcgagctc cttcaaccgc
     3481 tgcaacaaca cgacgtagag gcaatctggc tgggcgatat acaagaacat cctgcaatcg
     3541 cgcgcagatt tgtgaattag cggcccgggt attccgggtt actgaaagga tatccattga
     3601 ggttggtatg aatattttca cagaggctgc aaaactcgaa gagcaaaatt gtccgtttgc
     3661 gatggcgcaa attgttgata gccgaggctc gactccccgc cattctgcac aaatgttagt
     3721 gcgcgccgat ggttctatcg tcggtacaat tggtggcgga atggttgagc ggaaggtgat
     3781 tgaagagtca cttcaggcat tgcaggaacg taagccgcga ttattccatg gacgtatggc
     3841 tcgtaacggt gcggatgctg tcgggtcaga ttgtggaggt gcaatgtcag tatttatcag
     3901 cgtccatggt atgcgtccac gtctggtgtt gattggcgcg gggcatgtca accgggcgat
     3961 agcccagagt gcggcgctat taggatttga tattgccgtt gccgatattt atcgcgaaag
     4021 cctcaatcct gaactattcc caccatcaac cacgcttctc catgctgagt cgtttggtgc
     4081 ggcagtggaa gaactggata ttcgccctga taattttgtc ctgattgcca cgaataatca
     4141 ggatcgtgaa gccctcgaca aactcattga acagcccatt gcatggttgg ggttgctggc
     4201 aagtcgccgc aaggttcagc ttttcctgcg tcaattgcgt gagaaaggcg tggctgaaga
     4261 acatattgcc cgtttacatg cgcccgttgg ttacaacata ggtgcggaaa cgccgcagga
     4321 gatcgccatc agcgtgctgg cagaaatatt atgggtgaaa aataacgcgc cgggtgggct
     4381 gatgatgaaa ccttctcatc cttccggaca ccagctggtg gtgattcgcg gtgcgggaga
     4441 tatcgccagt ggtgtggcgc tacgtctgta tcatgcgggt tttaaagtga tcatgctgga
     4501 agtggaaaaa ccgacagtga ttcgttgtac cgtggcgttt gcccaggccg tgttcgatgg
     4561 cgaaatgacg gtcgaaggcg tcactgctcg cctggcaacc agctctgcgg aagcgatgaa
     4621 acttaccgaa cgcggattca tccctgtgat ggtagatccc gcctgttcat tgcttgatga
     4681 actgaaaccg ctttgcgtgg tggacgctat tctggcgaaa cagaatttgg gaacgcgggc
     4741 agatatggca ccagtaacaa tcgcgcttgg gccgggcttt actgcaggga aggattgtca
     4801 tgcggtaatt gaaacaaatc gcgggcactg gctcggtcag gtgatttact ctggttgtgc
     4861 gcaggagaat accgatgttc ctggcaatat tatggggcat accacccgac gggtgatccg
     4921 tgctcctgct gcaggcatta tgcgatccaa cgtgaaatta ggcgatctgg tgaaagaggg
     4981 cgatgtgatt gcctggattg gtgagcatga aattaaagca ccgttgacgg ggatggtgcg
     5041 tggcttgttg aacgacggcc tggcagtggt tggtggtttt aaaattggtg atatcgatcc
     5101 tcgtggtgaa accgctgatt tcaccagcgt ttctgataaa gcccgggcga ttggcggcgg
     5161 cgtacttgag gcgttaatga tgttgatgca tcagggcgtg aaagcgacaa aagaagtgct
     5221 ggaagtggct taaatgaaaa tggccggatg gtattaccca cccggcctta aaattactga
     5281 caaatcaccg ttccggtttt gccttcaatc ccttctttcg ctttgctcaa tacggtaatc
     5341 agtgcttcac gacctgcgcg agagcgaaca aacgaggcgg cagcttccac tttaggcagc
     5401 atagaacctt tcgcaaaatg accttcttcg ataaagcgtt cagcatcact taatgacagg
     5461 cgatcgagcc actgttcatt ctctttaccg aagttaatgg ccactttttc taccgcagtc
     5521 agaatgatca gcatatcggc atcaatcatt tctgctaaac gggcgctggc ccagtcttta
     5581 tcgataaccg cgctggcacc acgcagatgg ttaccttcac gaataacagg gataccacca
     5641 ccgccaacgg cgatcaccac ctggcctgca tctaccagag ctttaaccgt ttctttttca
     5701 atgatatcaa ccggttttgg cgaggcaaca acacggcgat agccgcgacc agcatcttct
     5761 ttcagagtgt aaccctgttt tgtcagttgc tctgcttctt gctcggtaaa gaacgaaccg
     5821 atcggcttgg tcgggttgag gaatgccgga tcgttagcgt cgacttcgac ctgagtaacc
     5881 agcgttgcta caggtttatt aatgccccga gaaagcagtt cttctcttaa cgcgttttgt
     5941 agatcgtaac caatataacc ctggcttaag gcaacacaga cagacatcgg cagcattggc
     6001 gagtgtgctt cagtttttgc ggcagcttca aaggcttggt taatcatgcc cacctgcggc
     6061 ccgttaccat gagtgacgat aacttcatgt ccttgggcaa ttaaatcaac aattgcctga
     6121 gaagtaattt ttaccgcttt catttgtccg gccaggtcat cgcccagcgc attcccgccc
     6181 agggcgagaa caattttctt actcattttt cctcactcat cattcttgat tgtgtataga
     6241 tccctgacag tggttagagc acgggaggga caaacggttt gcgtcgcagg aaacgacctc
     6301 gcccggcttt gcctgtaaag gtgccgtcac agaaaatcgt ttcaccacgg gataaggttc
     6361 tgacaatcgc gccctgacag gtaaaaccct cccatggcga gtagtcggcg ttgtcgtgga
     6421 gatggcgatg ctgaatttgt tggctctgac gtgggtcgat aatcaccacg tcgccatcgg
     6481 aaccgggcgc taataatcct ttttgcggcc acaggccaaa cagcctggcg ggcattgcgc
     6541 tggttaattc aacaaagcgt tccggtgtta tacgtcccgt catcacgcca ctggaaaaca
     6601 gtaactgcat gcggttttcc acaccgggta agccatttgg gcagcgactg aaatcgcctt
     6661 tagaaatttg caggcgttga gccatcgaga aggtgcagtg atcggtcgcc accacgtcaa
     6721 tcgcaccatc gctgatgcca caccacagtt tgtcctgctc gcgtacgtta cgcagcggtg
     6781 ggctaagaat gaacttcatg ccatcttctg tatcgtaact gcgttcgtcc aacaggagat
     6841 attgtgggca ggtttcaacc cagactggct ggtgattcgc acgggcaaga cgcagataat
     6901 ccagacctaa gccgttagac aggtgcacga tatagagcgg ggcgttaccg gcaatttgtg
     6961 ccaggttaat catgcgggcg atggcttccg cttcgcattc cagagggcga ctcaaggcat
     7021 gatagcgcgg cgcggttaac cctgcggcga taaattccgc ccgcttgctg gcgatagccg
     7081 catcattttc cgggtgcacg gtggtcagcg cgccggattc atgcaagcgg cgtaatgcct
     7141 gcaaaacctc gtcatcgttg agtttgtatt gataggttaa atagagttta aaactgctca
     7201 gtccttcctc gaccatcatc gggatttcgt cgaggattgc gtgattaatg tgctggatca
     7261 caccgtgaaa gctgtaatcg atgaccgctt tatgggcggc ataaccacga taaacctcca
     7321 gttgatggcg taaccgacag ccgtttgggc caaatcccat atggtcaata atggttgttg
     7381 taccgccaca cgcagctgcg cgggtaccgg taaaaaatca tcacaactgc gcgcgatgcc
     7441 gacatcaata ttgaaatgcg tatggacatc cacgccgcca gggaaaacgt aacagccagt
     7501 ggcatcaatt tcttcatacg ggagctgcgg cgaaatattg ttgcccaact ggcgaacaat
     7561 cccgctttca atcagcaaat cctgtttggc ttgtccatct gcgttaacga cagtgccgtt
     7621 tttgatcaat acgcgcatag caaactccaa aaaccctccg gcgaaccgga ggggaataag
     7681 tgaagagtaa ttactctgtt gccagccagc ttaacgggat tgcagcgtac atcgcggcac
     7741 aggtcaccag gtgagatttc caggtttttt cgttaggtgc atgcgcttca ggttctttac
     7801 ccgggccaaa gccgatcacc ggaatgccgt gacggcccat gatagatacg ccgttagttg
     7861 agaaggtcca cttatcaaca accggcgctt tgccaaacag cccttcgtag gcattcacca
     7921 gtgctttaac ggtgaagtga tcttcttcca ctttccaggt cgggaagtag cattcggttg
     7981 ggtaaaccag gccagtccag gacggacggt cgtagttgta catagaaaca accgcgttag
     8041 ctttctgtac tgcaggcagg gcgcggattt cgtccagcgc gccttcccag gtttcgcccc
     8101 aggtcagacg gcggtcaata gagacggcgc agctatctgc tacagcgcaa cggcttgggg
     8161 atgtgaagaa gatttcagaa acggtgaggg tgcctttgcc gaggaattcg tcataaccca
     8221 gacgttggga gagttcttgt aattcgccaa gaatcggacc cattttgaaa atggcgttgt
     8281 caccgcgttc tggcgcagaa ccgtggcagc taacaccctg aacatcaata cgaatttcca
     8341 tacgaccgcg ctgaccacgg tatatctggc agtcggttgg ttcggtactg accacaaatt
     8401 ccgggcgaat gccggattgt tcaataatgt actgccagca cagaccgtcg cagtcttctt
     8461 cctgcacagt accggtaacc agcagggtat attcatcttc cagaccgagg tctttaatga
     8521 ttttaccggc ataaaccata gatgccatgc cgccttcctg gtcggaagta ccgcgaccac
     8581 cgattagctc atcagtttcc atgccttcgt acggatcgaa gtcccagttt ttgatgttgc
     8641 caatgccgac ggtatcgata tgagcgtcca ttgccaccag acgcgggccg tggccgatat
     8701 aaccgagaac gttgcccatc gggtcgattt caactttatc gaagccgact ttttccatct
     8761 cttctttaat acgatgtact acgcgttttt cgtcgcagct ttcactggga atagcaacca
     8821 tgtcgcgcag gaagcgagtc atgtccgcct ggtaatcttt tgctttttca agaatcagtt
     8881 tgaatggaat attcttagcc attatttttc tccagatttt ttactccccg ttttgcaggg
     8941 agtagttcca atagggtgat taaggtgcta cagcgtgttt gccttcccag acaacttcgc
     9001 ggtagtgctt cacgtcggtg tcgccttcag tgctgataac cagcactacg gcatctttgt
     9061 ccagcgccag tttttccatc aggctttgat gttgcgggtg ataatgaacc gctgcgagaa
     9121 cgcccaaacc gacagcgccg gattcaccgg agatgatgcg cgggtcgttg ccgtacggat
     9181 tacccagcac gcgcatacct aatgcggcaa cgctgtcctg gcaggagatg aattgggtgg
     9241 cgcagttacg taggatttcc cagcccagcg ggttaggttc gccacaggcc aggcccgcca
     9301 tgatggtggc catatcaccg ccaacgttga cgatgtcgcc tttgacgccg gagcgataaa
     9361 tacagtcagc tttgtcaggt tcaacaataa tgctgtgcag attttgcggg ctgtagacgt
     9421 cgaccagata acccagcaca ccaccggcca ttgctccgac accggcttgc agcagaacgt
     9481 gcgtcggggt tacgcccatt tcacgcattt gctcgacggc ttcatctgcc agggttgcgt
     9541 agccttgcat gatccaggtt gggattttgg tgtaaccttc ccatgccgtg tcctgtacca
     9601 cttcccagcc gtgctgctgc gcgtgttgca tggtcaggcg aacggtatcg tcatagttca
     9661 tatccgtgac gatgcactcg gcaccgaggt tcagaatggc gtcaacgcgt tcctgagcag
     9721 aacctttcgg catgtaaatc accgcattct gtccgagttg ctgtgctgcc cacgccacac
     9781 cgcgcccgtg gttgccgtcg gtggtggtcg cgaaagtcat tttttcgccg atggcatttt
     9841 tcaggtgctc aaatgacagc gtttcgatat caagatgata tttttcacac aataattgag
     9901 cgatggcgta cgcaccgcca agcattttga aggcgttcag accgaatcgt tttgattcgt
     9961 ctttgacgag aattttcttc acaccaaaaa ggtttgcgag atcgtccagc gcacaaagcg
    10021 gtgttgggcg ataaccagct attttctggt ggaactggcg cgccagtttg gcctggcttt
    10081 gcgaaaagag cggtgatgtt tcgccgttga aaaatttgtt atcggcgata tcaatcttca
    10141 atgagaaaac ggacatatcc tatccttttg gcctctggaa atagagggcc gaccagcaga
    10201 tcggcccgag gggttatttc acgcgttctt gcgcttcttt cagcagttgc tcgagcagtg
    10261 cacctggttt ggcgtattta cgggacagga tcatcgcggc gatgatataa ggcttccagc
    10321 tggcttcttt gtaggtagcg atacggtatt tttcgaatac gccttcagtc acttcacctt
    10381 ctttgcagga tacgccgctg atatcagctg gcaggcagtg catgtacagg gcttcgccat
    10441 cacgggtcag ttccatcatc tcttcagtac aatgccagtc tttgtgttgc gcgttctgtg
    10501 ccagacactg tttttccagt gcttttaagc cttcgtggtc gttcgcacgc agcaattcag
    10561 tacgctcttc catcactttg taaggtgccc atgacttcgg ataaacgatg tctgcgtctt
    10621 tgaaggcttc ttccatgctg gtgacctgac ggaagctacc accggaggct ttagcattgt
    10681 ttttcgccac ttcaaccaca tccgggatca ggtcgtagcc ttccggatgg gccaaggtga
    10741 catccatacc gaagcgagtc atcagaccga tgatgccttg tggtacagag agcggtttgc
    10801 catagcttgg agagtaggcc caggtcatgg cgattttttt acctttcagg ttttccagtg
    10861 aaccaaagtg ttcacgtaac cacgcgaggt cagccattga ctgagtcggg tggtcaatat
    10921 cgcattgcag gttcactaaa gccggacgct gtggcagtac accctgcttg taaccgtcat
    10981 caagtgcagc gccaacttca cgcatatagg cgttgcctgc gcccagatac atatcgtcgc
    11041 gaataccaat agcgtcggcg cagaaggaga tcatattggc ggtttcacgc acggtttcgc
    11101 cgtgagcgat ttgtgatttg ccttcgtcga gatcttgttg tgcaaggccg agcaggttaa
    11161 gcgcggaagc ataagagaag cgggtacggg tggagttgtc gcggaatacg gaaataccta
    11221 atccactatt aaagactttg gttgagatgt tttcagcacg cagtgctttt aatgctgcgg
    11281 caacgtccag tacttgtttc agttcatctg gcgtctgttc ccacgttaac aaaaaatctt
    11341 tctcgtgaag gtgagaggtc agcgaattga tatccttaat cagctcatta acagttttca
    11401 tcatacagtc ccggtagtgg aatgacaggc aaagtggttt atttcactgt gacttgccaa
    11461 agtgattggt gttttcattg ctatacctgg gactaacaat aagtgtgcca gaattatccg
    11521 cgcggctgca aaagactaat ttgacggata atgtgtgggg ttgttcacaa attgatgcca
    11581 gcttctggtt gtttgtaaaa tacgaaaacg tttggctttt tttatcgcgt tatttatttt
    11641 ctgctaagta tcagaaataa gataaagggg caggctatct ctcttcagtt tgataaactc
    11701 accgccgtaa ttctcatatt gatatagaaa tgtgatatct cttccataat tgcattagaa
    11761 aatgataaaa atcttatgtt tttatcaaag ccaatttcgc ctgagttaat atcatcatga
    11821 tctgtgaacc atcaacgtct tcaatgatcg acttttaagt aagtctggaa ggtaaagcta
    11881 tggagcttgc tactacgcag tcagtattga tgcaaattca accgacaatt cagcgttttg
    11941 ccagaatgct tgccagcgtt ttgcagtttg aggttgagat cgttgatgaa aacttgtgtc
    12001 gcgtcgccgg aacgggcgcg tatgggaagt ttcttggtcg ccagttgagc ggcaactcac
    12061 gcctgctccg ccacgtcctg gaaacgaaaa ctgaaaaagt tgtgacacag tctcgcttcg
    12121 atcccctttg cgaaggttgc gatagtaaag aaaattgccg cgaaaaagca tttctgggta
    12181 cgcctgtcat tttacaggat cgttgtgttg gggtgataag tttgattgcc gttacccacg
    12241 agcaacaaga gcatatcagt gataatttac gcgaattttc tgattatgtt cgccatatat
    12301 ccaccatttt tgtttcgaaa cttctggagg atcaggggcc aggagataac atcagtaaaa
    12361 tattcgcgac catgatcgat aatatggatc agggcgtatt agttgttgat gatgaaagtc
    12421 gggttcagtt tgttaatcag actgccttaa aaacacttgg tgttgtacaa aataatatta
    12481 ttgggaaacc tatccgtttc agaccattaa catttgagag taattttact catggacata
    12541 tgcagcatat tgtttcgtgg gacgataaaa gtgaattaat cattggtcaa ttgcataaca
    12601 ttcagggccg acaattattt ttaatggcat ttcaccaatc gcataccagt ttttctgtag
    12661 caaatgcacc tgatgaacca catattgaac aattggttgg cgagtgccgt gttatgcggc
    12721 aattaaaacg actcattagc cgtattgcac ccagcccatc cagcgttatg gtggttggtg
    12781 aaagcggcac gggtaaagaa gtcgtcgccc gagcaatcca taagttgagc ggaagacgga
    12841 ataaaccctt tattgctatc aactgtgccg cgattccgga gcagcttctg gaaagcgaac
    12901 tgttcggtta tgttaaaggc gcatttactg gtgcttctgc caacggtaaa acagggttga
    12961 ttcaggcggc gaatacgggc acgctgtttc tcgatgaaat aggtgatatg ccattaatgt
    13021 tgcaggctaa attactgcgc gctattgagg cgcgtgaaat tctgccgatt ggtgccagta
    13081 gcccaataca agtcgacatt cgcatcattt ctgcaactaa tcagaatttg gcccagttca
    13141 ttgccgaagg taaattccgc gaagatctct tctaccgact taatgttatc ccgataactc
    13201 tgccaccgct gcgtgaacgt caggaagata ttgaactatt ggtgcattac tttttacatc
    13261 tgcatacccg tcgtctggga tcggtttatc ctggcattgc tcccgatgtc gtcgaaatat
    13321 tgcgtaagca tcgttggccc ggaaacctgc gcgagttaag caatttgatg gaatatctgg
    13381 ttaacgtggt tccttcaggt gaagttatcg acagcacgct attgccgcca aatctgctga
    13441 ataatggcac aacggagcaa agtgatgtaa cagaggtcag tgaggcgcac ctgtcactcg
    13501 atgatgcggg cggcacggcg ctggaggaga tggaaaagca aatgatccgc gaggcgcttt
    13561 cacgtcataa cagcaagaag caagttgctg atgaactggg catcggcatt gctacgctct
    13621 atcgcaagat taagaaatat gagttgttaa acacataacc tcagtgaatc cggcctgaat
    13681 tcaggccgga tatcctttta cttcgttttc tcgcaatcca gaactgtatt tacaatcatc
    13741 tgataccccg tgcagcgaca aagatttccc gccagtccgc gacgaatttc cgtaatggtt
    13801 aatggcttct cgcgtggttt cgccagcatt gccgtggtag ccataatcag gccaggcgta
    13861 caaaacccgc actgcactgc gccggatttc gcataagcct gctgaacatg agaaagtttt
    13921 ccgcctttcg cttcaccttc cagcgtgcgg atctcctttc cttcagccca ggcggcaagg
    13981 tataagcaac tgtctattgc tgtgccgtcg accaacaccg tacaggcacc acattcaccc
    14041 acgcagcacc cttgtttgac acttagcagt ccttgttcgc ggagtaattc cgagagcggc
    14101 gtgcctggtg cggcgtgaag ctgaaaaggc atcccgttaa tggtgcattc gatggtaatt
    14161 gtttcgctgt gattcattgc aattttcccc ccgccgcggc gacggcttcg ctaatcactt
    14221 ttttggtcat cgtctggatg agatgcagac gaaactcttt actggcccgc catgaagaac
    14281 gcggggcgac atcttgcagg acagattcgc tgatagcttc cagcgtttgc aggtttaatg
    14341 gcgcattttg tgcagtctgt tcggcatgtt ggcagcgaat cggcgttggc gcggcaacac
    14401 caaatgccag gcgtaattcg ctgaaattgc cgttatccag tcggcaatgt gcggcgcagc
    14461 caatcgttga aatatccatt gcgtcgcgca tggcatattt aaaatgcgcg ctgcccgcgt
    14521 gttctttcgg ctgtggcgga aaatgaaagg cgacgaggat ttcgtcatgc tcaagagaca
    14581 ctttgcccgg cccggtgtga aagccattaa tcgggacgaa acgaacaccg cgcggggagt
    14641 ggatctccag tttcgcgtca taaattagcg ttggcgtggc agaatctgcg ctggtggcac
    14701 cgttgcaaat atttccaccg taggtagcga cgttacggat ctgcggtcca gcaatggacg
    14761 tggccgcagc acataacgcc gggagatgac gttgagttat aggagcttct attagctggg
    14821 taaatgtcgt tgcagagccg atacgtagcg agccatcttc cgccagcgta attccccgca
    14881 gctccgccag attatgaata tcaacaatat ggcgataacg gtcattgtga tggtggagct
    14941 gaatcagtac gtcagtgcca tcggcgagca gtttggcctg cgggttgtca gccagcaggt
    15001 tgatggcatc ggcaagggtt gctgcgcgat ggtaagaagc aaaatcaaac atgatgttat
    15061 cctcaaatca atcctgccag atggaactct tcatataacc gttttggcgt cagcggcagt
    15121 gtattgattg caacaccggt agccatcttc accgcgttac gaatagcagc ggcaacagga
    15181 attattggtg gctcacccag tgacttatgt ccgtatgcgg attgcggctc attgatttcg
    15241 acgaacgcgc tttccagttg tggcagatcc ggcatggtcg gcattttgta atccagcaga
    15301 ttggggttac ggaccacgcc gcttttagca tcgatgatca tctcttcaaa tagcgcccag
    15361 ccaatgccca ttcccattcc gccgtgtacc tgaccttctg ccagcagtgg attaagaata
    15421 tgccctgaat catgaacgtt gaggatgcgg ttgatggtga ctttgcacag cgcaatatcg
    15481 accgtcagat caacaaaggt acagccaaac gccgatgggt tagtggtggt tttgatggag
    15541 ctttcagcag agagctgccc gccgcgttca gggtggtaga aagcgtccat cgccaaatct
    15601 tttaacgaca ttaacggctc ttccggtcgt tcaaccagca cgatatggcc ttttatcagg
    15661 gtcagattca tcgctgactg atgtagcatg actgcggcgt gagcgatgat tttctctttt
    15721 aataatagtg ccgcactgcg cagcgcaggc gcggcaacat agctctggcg tgaggcaaat
    15781 gcgccgggag cgaacggcgt aacgtcggta tcttgagttg aaataacgcg aacgtcgctg
    15841 accggaaccc ccacggtttc tgccaccatt tgcgagaaga cggtgtcggc accctgaccg
    15901 atttccgtcg cgccgctttg cacgttgatg gttccatcct gattcatcag aaggcgcgcg
    15961 cctgctattt ctacgccgac aggccaggtg ttagaggtat agctaaaaca ggcgacgcca
    16021 acgccgcggc gcaaattgcc ttgctggttc tggcattctg cacggcgttt ttcccattca
    16081 aagattttcc ggcctttttc aagacactcc ggcaaccctg cgctgtaaat acgtttgccc
    16141 gtgagcggat tagcatctcc ttcgcgggcg gcgttgcgta aacgaatttc aacaggatca
    16201 atacctaacg ctgtcgcggc gtcatcaagc atagactcaa cggcaaatac gacttgtggc
    16261 gcgccataac cacgcatcgc accagccgag gggaggttgg tatagcaggt ctttgaactg
    16321 taagcgtagg cacaacgagg ataaaggtaa gcgactttat tcctccccgc agaagcgatg
    16381 gagtgcccgt gagatgcata agcgccggtg ttagacagaa catccagact ataacctttc
    16441 aatgttccgt cgcggttcac gcccatttgc ccgtcaatgg taaaagcgtg gcgggtacgg
    16501 gttgcgagga aacactcttc acggctaagg gaaactttca ccggaatgcc gccaagcttg
    16561 ctggtcagga atgctgccat tggctcttcc agtacatcct gtttattacc aaaaccgcca
    16621 ccgacaaacg gtttgatgac tcgtacgcat gaccagggaa tatccagcgc ctgaccaacc
    16681 acgcggcgaa caatgtgcgg gatctgggtg ctggaaacga tggtaattcg cgagtcatcc
    16741 tccatccacg ccagcgatgt tacgctttcc atgtgacaat gttgaataac gggggtctga
    16801 tagtgcccct gtacctggta gtcggcggca tcgattgttt gttggacatt acccgtcgac
    16861 atcgtgcttt gtttcagtaa attgccaccg ttatggattg gtgcagcgtc ttctgccagc
    16921 gccgcttctg gcgtggtgat aacgggtaat tcttgccact caatgctgac caattgcgcc
    16981 gctttttctg ccgtgagttc atcgcgggcc acgacgatgg caacggcgtc accatgatga
    17041 cgaacatggc gagttagcag tgcgcgatcg gcggtatcgc gcttgttttc gtcaagtgtc
    17101 caggcatgcc cagctgtagc gaatggaata tcaggcacat cttcccaggt aaaaatcgcc
    17161 agtacgcccg gcaaacttct ggcttgttca tcattaatac ttacggcata accatgtgcg
    17221 atagggctac gtacatattt cgcataacac atgcccgcca taacataatc gtcagtatat
    17281 cgtgcccgcc cggtgacctt agcaatggca tcgacgcgca tgcatgattc acccgtagcg
    17341 gttgcttccc gcgcttccat agaaatcccc tcgatttgaa catttggttc ttatggcaaa
    17401 gatttagtct aagcaggcaa gatttacgcc agaaacgacg ataagcgtta ggggaggtag
    17461 gaagtgtgat gacgttaaaa aaacacagat atgatacgaa acaggactct gaataggggc
    17521 agtgatacgt atcaatatga gtggcgataa aatcaatgtg ataaattggg attattacgc
    17581 atcaggatgg gaaatagggt aaaaagtcag gaagttcgat acctctacag cgaatatttt
    17641 gctgatgata cttccctttg ccaaatgaaa ttaacctctt tctctattac cacgtttttc
    17701 cagaagcaag agattgggta tcaagaggct ggctgctatg ataaggcgtc ttgtttttta
    17761 agcgaggaaa gattttgagt gcgggacgcc tgaataaaaa atctctgggt atcgtgatgt
    17821 tgttatcggt tggactgctt ttggcgggct gttcgggtag caaatcatcc gatacaggaa
    17881 cgtattccgg ctccgtttac accgtgaaac ggggggatac gctatatcgt atttcgcgca
    17941 ccacgggaac cagcgtaaaa gaactggcgc gactgaacgg catttccccc ccttacacca
    18001 ttgaagttgg tcagaaacta aaactgggtg gggcgaaaag tagcagtagt acacgtaaat
    18061 caaccgccaa atcaacgacc aaaaccgcat cggttacacc gtcatcagcg gtaccgaaat
    18121 catcctggcc gccagtaggg caacgttgtt ggttatggcc aacgacaggg aaagttatca
    18181 tgccgtattc gacagcagat ggcggcaata aagggattga tatctcagct ccacggggta
    18241 cacctattta cgccgcgggt gcaggaaagg tggtgtatgt gggcaaccag ctgcgtggct
    18301 acggtaatct catcatgatt aaacacagtg aagattacat tacggcttac gcccataatg
    18361 acacgatgct ggtaaataat gggcaaagcg tgaaggctgg gcaaaaaatc gccactatgg
    18421 ggagcacgga tgcggcatct gttcgcctgc atttccagat tcgttaccgt gcaacggcaa
    18481 ttgatccgct acgttacttg ccgccgcagg gcagcaagcc aaaatgctga tggcgaatta
    18541 atcagcagtc agcagcgcgg cacttgctaa ggagagcgta aggtttataa tgccttacgc
    18601 atctcgaagc gggcgtagtt caatggtaga acgagagctt cccaagctct atacgagggt
    18661 tcgattccct tcgccgcttt tgctgtatct gattttctat attatcttaa ttcaatcgct
    18721 tcagactttt cgcaatcgcc gtaatatttc cccattttgt tgtaaataaa aagcgtttac
    18781 gcaatgcaat ccttttgtaa caacccaggt aaaacacctt attttcaagt tgttaaataa
    18841 gtggttattt acgcttttaa caaacgattg atggaatatt ttatgaaagg gaaatctgca
    18901 ttaactcttc tgctcgctgg tatattttcg tgcggcacat gccaggcgac aggggcagaa
    18961 gtgacgagtg aatctgtatt taacattctt aattccacgg gggcagcaac agataaaagt
    19021 tatttatcgt taaatccaga taagtaccct aattatcgtc ttctgattca ctcagccaaa
    19081 ttaaaaaatg aaataaagag ccactatacc aaagacgaaa ttcaagggtt gttaacatta
    19141 acagaaaaca cacgaaagtt aacgttgacg gagaaaccct ggggaacatt tattcttgct
    19201 tctactttcg aagatgacaa aacagcggca gaaacccact atgatgctgt ctggctgagg
    19261 gatagtttat ggggctatat ggcattagta tcagatcagg gaaatagtgt agccgcaaaa
    19321 aaggttctgc tcaccctgtg ggattatatg tctacgctcg accagattaa gcggatgcag
    19381 gacgtcatta gcaaccccaa acgactggat ggagttcccg ggcaaatgaa tgctgtgcat
    19441 atccgatttg acagcaactc ccccgtgatg gcagatgtgc aggaagaagg caagccgcag
    19501 ctgtggaacc ataaacaaaa tgatgcgtta ggtctttatc ttgatctcct tatacaagca
    19561 atcgacactg gtacaattaa cgctgaagat tggcaaaaag gtgaccgatt aaaatcggtt
    19621 gcgctgctga ttgcttatct ggataaagcc aatttctatg ttatggaaga ttccggagca
    19681 tgggaagaag atgcccgatt aaacacatct tccgttgcac tggtgacctc tggactggaa
    19741 cgtttgtcga acttactctc gaagaaagac tctgtttttg tctctgattt actacgcgaa
    19801 gctaaagcca atgaactgga tgaacctctt tcaacaacga ggttgaacca cctcatagat
    19861 aaaggttatg aaagaataac gttacagctt gatttagggg gagaatcccc aggttatctg
    19921 gaaaaagata aacattacag agaagccgat gcggctttac ttaatgttat ttatcctgca
    19981 aatcttgcaa agataaacac cagaagaaaa gagcaggttc taaaaatagt taaaaaacta
    20041 gcaggtccgt atggtataaa aagatacgag aaggataatt atcagtcggc aaatttttgg
    20101 ttcaatgata ttaaaaccga taccgatcaa aattcacatg cgaaaag
//
LOCUS       DAZPAC010000059        20140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZPAC010000059 DAZPAC010000000
VERSION     DAZPAC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20140
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.059"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(204..1016)
                     /locus_tag="ACHUPM_001817"
     CDS             complement(204..1016)
                     /locus_tag="ACHUPM_001817"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR0869606.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            1278..2039
                     /gene="udp"
                     /locus_tag="ACHUPM_001818"
     CDS             1278..2039
                     /gene="udp"
                     /locus_tag="ACHUPM_001818"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR0869607.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2180..3607
                     /gene="rmuC"
                     /locus_tag="ACHUPM_001819"
     CDS             2180..3607
                     /gene="rmuC"
                     /locus_tag="ACHUPM_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR0869608.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3702..4457
                     /gene="ubiE"
                     /locus_tag="ACHUPM_001820"
     CDS             3702..4457
                     /gene="ubiE"
                     /locus_tag="ACHUPM_001820"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR0869609.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4471..5076
                     /gene="ubiJ"
                     /locus_tag="ACHUPM_001821"
     CDS             4471..5076
                     /gene="ubiJ"
                     /locus_tag="ACHUPM_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR0869610.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            5073..6713
                     /gene="ubiB"
                     /locus_tag="ACHUPM_001822"
     CDS             5073..6713
                     /gene="ubiB"
                     /locus_tag="ACHUPM_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR0869611.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6792..7061
                     /gene="tatA"
                     /locus_tag="ACHUPM_001823"
     CDS             6792..7061
                     /gene="tatA"
                     /locus_tag="ACHUPM_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR0869612.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7065..7580
                     /gene="tatB"
                     /locus_tag="ACHUPM_001824"
     CDS             7065..7580
                     /gene="tatB"
                     /locus_tag="ACHUPM_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR0869613.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7583..8359
                     /gene="tatC"
                     /locus_tag="ACHUPM_001825"
     CDS             7583..8359
                     /gene="tatC"
                     /locus_tag="ACHUPM_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR0869614.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8401..9183
                     /gene="tatD"
                     /locus_tag="ACHUPM_001826"
     CDS             8401..9183
                     /gene="tatD"
                     /locus_tag="ACHUPM_001826"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR0869615.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(9180..9668)
                     /gene="rfaH"
                     /locus_tag="ACHUPM_001827"
     CDS             complement(9180..9668)
                     /gene="rfaH"
                     /locus_tag="ACHUPM_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR0869616.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9835..11328
                     /gene="ubiD"
                     /locus_tag="ACHUPM_001828"
     CDS             9835..11328
                     /gene="ubiD"
                     /locus_tag="ACHUPM_001828"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR0869617.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11375..12076
                     /gene="fre"
                     /locus_tag="ACHUPM_001829"
     CDS             11375..12076
                     /gene="fre"
                     /locus_tag="ACHUPM_001829"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR0869618.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12266..13429)
                     /gene="fadA"
                     /locus_tag="ACHUPM_001830"
     CDS             complement(12266..13429)
                     /gene="fadA"
                     /locus_tag="ACHUPM_001830"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR0869619.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(13439..15628)
                     /gene="fadB"
                     /locus_tag="ACHUPM_001831"
     CDS             complement(13439..15628)
                     /gene="fadB"
                     /locus_tag="ACHUPM_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR0869620.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15818..17149
                     /gene="pepQ"
                     /locus_tag="ACHUPM_001832"
     CDS             15818..17149
                     /gene="pepQ"
                     /locus_tag="ACHUPM_001832"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR0869621.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17149..17763
                     /locus_tag="ACHUPM_001833"
     CDS             17149..17763
                     /locus_tag="ACHUPM_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR0869622.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17802..19253
                     /gene="trkH"
                     /locus_tag="ACHUPM_001834"
     CDS             17802..19253
                     /gene="trkH"
                     /locus_tag="ACHUPM_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR0869623.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19265..19810
                     /gene="hemG"
                     /locus_tag="ACHUPM_001835"
     CDS             19265..19810
                     /gene="hemG"
                     /locus_tag="ACHUPM_001835"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR0869624.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
BASE COUNT         4704 a         5109 c         5483 g         4844 t
ORIGIN      
        1 tttgtgaatg ggagttgatg gtaaatctct aaggtgtctt ccattgaaga gactcattta
       61 tgactatgac ttgccgactt tcttaaggtc aaatagcgga gtcgtaatct ccaatcaatt
      121 catactgatt attttttggg ggacagagat ggcagcaaaa cgcactcgtt tactcaccat
      181 ctcaaatata gactaagagg aaactaactc cgcttctctc catactgctt aaaccattcc
      241 agcatacgct gccagccatc tttcgcagat tcggcatgat agctcgggcg ataatcagcg
      301 ttgaatgcat gcccggcatc cgggtacacg ataatctctg ctttcgcatt agcagcccgc
      361 agcgcctggc gcatggtttc aacgctctcc tgcggaatgc tgttatcctg accgccatat
      421 aagccgagaa tcggcgcgtt aagatcggtt gcgatatcaa caggttgttt cggtgaattc
      481 agcgacttgt cgcccgtcag tttgccgtac cacgccactg cagcttttag ctgtggatta
      541 tgcgcggcat acagccaggt gatacgtcca ccccagcaga atccggtgat cattaaacga
      601 tgaacatcgc cgccgttgcg ggaagcccaa ctggcggcat gatcgagatc ggccagcacc
      661 tgcgagtcag gcacttttgc taccagaccg ctaagcaacg tggggatatc ggcaaaatca
      721 ttcggatcgc cttcgcggaa gtaaagttca ggtgcgatag ccagataccc ctccagcgcc
      781 agacggcgac aaatgtcgcg gatatgttca tgcacgccaa aaatttcctg cactacaatg
      841 accactggca gtgggccatc gctttgcttt ggtctggcat ggtaagcagg catgttatcc
      901 ccttgtgaag ggatagaggt gaagcccgcc acaatcgcgt cgtccagggt ctgaacgatg
      961 gtcgaagcga gaggcgatgc agcaggtgca aatccagatt gttgtgttgt tgccatggta
     1021 ttctccgtac ccttataaaa atgttgcgca atgttaacta tagtcagcat gcaacaaatc
     1081 acattgcctg aatcggctca tcttttatgc agtcctgcag aatgaagagt gatttatgtg
     1141 atttgaatca cttttggtgg gtaaatttat gcaacgcatt tgcgtcatgg tgatgagtat
     1201 cacgaaaaaa tgttaaaccc ttcggtaaag tgtctttttg cttcttctga ctaaaccgat
     1261 tcacagagga gttgtatatg tccaagtctg atgtttttca tctcggcctc actaaaaacg
     1321 atttacaagg ggctacgctt gccatcgtcc ctggcgaccc ggatcgtgtg gaaaagatcg
     1381 ccgcgctgat ggataagccg gttaagctgg catctcaccg cgaattcact acctggcgtg
     1441 cagagctgga tggtaaacct gttatcgtct gctctaccgg tatcggcggc ccgtctacct
     1501 ctattgctgt tgaagagctg gcacagctgg gcattcgcac cttcctgcgt atcggtacaa
     1561 cgggcgctat tcagccgcat attaatgtgg gtgatgtcct ggttaccacg gcgtctgtcc
     1621 gtctggatgg cgcgagcctg cacttcgcac cgctggaatt cccggctgtc gctgatttcg
     1681 aatgtacgac tgcgctggtt gaagctgcga aatccattgg cgcgacaact cacgttggcg
     1741 tgacagcttc ttctgatacc ttctacccag gtcaggaacg ttacgatact tactctggtc
     1801 gcgtagttcg tcactttaaa ggttctatgg aagagtggca ggcgatgggc ataatgaact
     1861 atgaaatgga atctgcaacc ctgctgacca tgtgtgcaag tcagggcctg cgtgccggta
     1921 tggtagcggg tgttatcgtt aaccgcaccc agcaagagat cccgaatgct gagacgatga
     1981 aacaaactga aagccaagcg gtgaaaatcg tggtggaagc ggcgcgtcgt ctgctgtaat
     2041 tctcttctcc tgtctgaagg ccgacgcgtt cggccttttg tatttttgcg tagcgcctcg
     2101 caggaaatgc ctttccaact ggacgtttgt acagcacaat tctattttgt gcgggtaagt
     2161 tgttgcgtca ggaggcgttg tggatttctc aatcatggtt tacgcagtta ttgcgttggt
     2221 gggtgtggca attggctggc tgtttgccag ttatcaacat gcgcagcaaa aagccgagca
     2281 attagctgaa cgtgaagaga tggtcgcgga gttaagcgcg gcaaaacaac aaattaccca
     2341 aagcgagcac tggcgtgcag agtgcgagtt actcaataac gaagtgcgca gcctgcaaag
     2401 tattaacacc tctctggagg ccgatctgcg tgaagtaacc acgcggatgg aagccgcaca
     2461 gcaacatgct gacgataaaa ttcgccagat gattaacagc gagcagcgcc tcagtgagca
     2521 gtttgaaaac ctcgccaacc gtatttttga gcacagcaat cgccgggttg atgagcaaaa
     2581 ccgtcagagt ctgaacagcc tgttgtcgcc gctacgtgaa caactggacg gtttccgccg
     2641 tcaggttcag gacagcttcg gtaaagaagc acaagaacgc cataccctga cccacgaaat
     2701 tcgcaatctc cagcaactca acgcgcaaat ggcccaggaa gcgatcaacc tgacgcgcgc
     2761 gctgaaaggc gacaataaaa cccagggcaa ctggggcgag gtagtattga cgcgggtgct
     2821 ggaggcttcc ggtctgcgtg aagggtatga atatgaaacc caggtcagca tcgaaaatga
     2881 cgcccgctcg cggatgcagc cggatgtcat cgtgcggctg ccgcagggaa aagatgtggt
     2941 gatcgacgcc aaaatgacgc tggtcgccta tgaacgctat tttaacgccg aagacgacta
     3001 cacccgcgaa agcgcgctac aggaacatat cgcgtcggtg cgtaaccata tccgtttgct
     3061 gggacgcaaa gattatcaac agctgccggg gctgcgaact ctggattacg tgctgatgtt
     3121 tattcccgtt gaacccgctt ttttactggc gcttgaccgc cagccggagc tgatcaccga
     3181 agcgttgaaa aacaatatca tgctggttag cccgactacg ctgctggtgg cgttgcgtac
     3241 tattgccaac ctgtggcgtt atgagcatca aagccgcaac gcccagcaaa tcgccgatcg
     3301 tgccagcaag ctgtacgaca agatgcgttt gttcatcgat gacatgtccg cgattggtca
     3361 aagtctcgac aaagcgcagg ataactaccg ccaggcaatg aaaaaactct cttcaggtcg
     3421 cggaaatgtg ctggcgcagg cagaagcgtt tcgcggttta ggagtagaaa ttaaacgcga
     3481 gattaatccg gatttggctg aacaggcggt gagccagaat gaagagtatc gacttcggtc
     3541 ggttccggag cagccgaatg atgaagctta tcaacgcgat gatgaatata atcagcagtc
     3601 gcgctagccc attaggggta gttaagccgg gtagaaatct agggcatcga cgcccaatct
     3661 gttacacttc tggaacaatt ttttgatgag caggcattga gatggtggat aagtcacaag
     3721 aaacgacgca ctttggtttt cagaccgtcg cgaaggaaca aaaagcggat atggtcgccc
     3781 acgttttcca ttccgtggca tcaaaatacg atgtcatgaa tgatttgatg tcatttggta
     3841 ttcatcgttt gtggaagcga ttcacgattg attgcagcgg cgtacgccgt gggcagaccg
     3901 tgctggatct ggctggtggc accggcgacc tgacagcgaa attctcccgc ctggtcggag
     3961 aaactggcaa agtggtcctt gctgatatca atgaatccat gctcaaaatg ggacgcgaga
     4021 agctgcgtaa tatcggtgtg attggcaacg ttgagtatgt tcaggcgaac gctgaggcgc
     4081 tgccgttccc ggataacacc tttgattgca tcaccatttc gtttggtctg cgtaacgtca
     4141 ccgacaaaga taaagcactg cgttcaatgt atcgcgtgct gaaacccggc ggccgcctgc
     4201 tggtgcttga gttctcgaag ccaattatcg agccgctgag caaagcctat gatgcatact
     4261 ccttccatgt gctgccgcgt attggctcac tggtcgcgaa cgacgccgac agctaccgtt
     4321 atctggcaga atccatccgt atgcatcccg atcaggatac cctgaaagcc atgatgcagg
     4381 atgccggatt cgaaagtgtc gactactaca atctgacggc aggggttgtg gcgctgcatc
     4441 gtggttataa gttctgacag gagaccggaa atgcctttta aacctttagt gacggcagga
     4501 attgaaagtc tgctcaacac cttcctgtat cgctcacccg cgctgaaaac agcccgctcg
     4561 cgtctgctgg gtaaagtatt gcgcgtggag gtaaaaggct tttcgacgtc attgattctg
     4621 gtgttcagcg aacgccaggt tgatgtactg ggcgaatggg caggcgatgc tgactgcacc
     4681 gttatcgcct acgccagtgt gttgccgaaa cttcgcgatc gccagcagct taccgcactg
     4741 attcgcagtg gtgagctgga agtgcagggc gatattcagg tggtgcaaaa cttcgttgcg
     4801 ctggcagatc tggcagagtt cgaccctgcg gaactgctgg ccccttatac cggtgatatc
     4861 gccgctgaag gaatcagcaa agccatgcgc ggaggcgcaa agttcctgca tcacggcatt
     4921 aagcgccagc aacgttatgt ggcggaagcc attactgaag agtggcgtat ggcacccggt
     4981 ccgcttgaag tggcctggtt tgcggaagag acggctgccg tcgagcgtgc tgttgatgcc
     5041 ctgaccaaac ggctggaaaa actggaggct aaatgacgcc aggtgaagta cggcgcctat
     5101 atttcatcat tcgcactttt ttaagctacg gacttgatga actgatcccc aaaatgcgta
     5161 tcaccctgcc gctacggcta tggcgatact cattattctg gatgccaaat cggcataaag
     5221 acaaactttt aggtgagcga ctacgactgg ccctgcaaga actggggccg gtttggatca
     5281 agttcgggca aatgttatca acccgccgcg atctttttcc accgcatatt gccgatcagc
     5341 tggcgttatt gcaggacaaa gttgctccgt ttgatggcaa gctggcgaag cagcagattg
     5401 aagctgcaat gggcggcttg ccggtagaag cgtggtttga cgattttgaa atcaagccac
     5461 tggcttctgc ttctatcgcc caggttcata ccgcgcgatt gaaatcgaat ggtaaagagg
     5521 tggtgattaa agtcatccgc ccggatattt tgccggttat taaagcggat ctgaaactta
     5581 tctaccgtct ggctcgctgg gtgccgcgtt tgctgccgga tggtcgccgt ctgcgcccaa
     5641 ccgaagtggt gcgcgagtac gaaaagacct tgattgatga actgaatttg ctgcgggaat
     5701 ctgccaacgc cattcagctt cggcgaaatt ttgaagacag cccgatgctc tacatcccgg
     5761 aagtttaccc tgactattgt agtgaaggga tgatggtgat ggagcgcatt tacggcattc
     5821 cggtgtctga tgttgcgacg ctggagaaaa atggcaccaa catgaaattg ctggcggaac
     5881 gtggcgtgca ggtgttcttc actcaggtct ttcgcgacag ctttttccat gccgatatgc
     5941 accctggcaa cattttcgta agctatgaac acccggaaaa cccgaaatat atcggcattg
     6001 attgcgggat tgttggctcg ctaaacaaag aagataaacg ctatctggca gaaaacttta
     6061 tcgccttctt taatcgcgac tatcgcaaag tggcagagct acacgtcgat tctggctggg
     6121 tgccaccaga taccaacgtt gaagagttcg aatttgccat tcgtacggtc tgtgaaccta
     6181 tctttgagaa accgctggcc gaaatttcgt ttggacatgt actgttaaat ctgtttaata
     6241 cggcgcgtcg cttcaatatg gaagtgcagc cgcaactggt gttactccag aaaaccctgc
     6301 tctatgtcga aggggtagga cgccagcttt atccgcagct cgatttatgg aaaacagcga
     6361 agcctttcct ggagtcgtgg attaaagatc aggtcggtat tcctgccctg gtgagagcat
     6421 ttaaagaaaa agcgccgttc tgggtcgaaa aaatgccaga actgcctgaa ttggtttacg
     6481 acagtttgcg ccagggcaag tatttacagc acagtgttga taagattgcc cgcgagcttc
     6541 agtcaaatca tgtacgtcag ggacaatcgc gttattttct cggaattggc gctacgttag
     6601 tattaagtgg cacattcttg ttggtcagcc gacctgaatg ggggctgatg cccggctggt
     6661 taatggcagg tggtctgatc gcctggtttg tcggttggcg caaaacacgc tgattttttc
     6721 atcgctcaag gcgggtcgtg taacgtataa tgcggccttg tttaatcatc atctaccaca
     6781 gaggaacatg tatgggtggt atcagtattt ggcagttatt gattattgcc gtcatcgttg
     6841 tactgctttt tggcaccaaa aagctcggct ccatcggttc cgatcttggt gcgtcgatca
     6901 aaggctttaa aaaagcaatg agcgatgatg aaccaaagca ggataaaacc agtcaggatg
     6961 ctgattttac tgcgaaaact atcgccgata agcaggcgga tacgaatcag gaacaggcta
     7021 aaacagaaga cgcgaagcgc cacgataaag agcaggtgta atccgtgttt gatatcggtt
     7081 ttagcgaact gctattggtg ttcatcatcg gcctcgtcgt tctggggccg caacgactgc
     7141 ctgtggcggt aaaaacggta gcgggctgga ttcgcgcgtt gcgttcactg gcgacaacgg
     7201 tgcagaacga actgacccag gagttaaaac tccaggagtt tcaggacagt ctgaaaaagg
     7261 ttgaaaaggc gagcctcact aacctgacgc ccgaactgaa agcgtcgatg gatgaactac
     7321 gccaggccgc ggagtcgatg aagcgttcct acgttgcaaa cgatcctgaa aaggcgagcg
     7381 atgaagcgca caccatccat aacccggtgg tgaaagataa tgaagctgcg catgagggcg
     7441 taacgcctgc cgctgcacaa acgcaggcca gttcgccgga acagaagcca gaaaccacgc
     7501 cagagccggt ggtaaaacct gctgcggacg ctgaaccgaa aaccgctgca ccttcccctt
     7561 cgtcgagtga taaaccgtaa acatgtctgt agaagatact caaccgctta tcacgcatct
     7621 aattgagctg cgtaagcgtc tgctgaactg cattatcgcg gtgatcgtga tattcctgtg
     7681 tctggtctat ttcgccaatg acatctatca cctggtatcc gcgccattga tcaagcagtt
     7741 gccgcaaggt tcaacgatga tcgccaccga cgtggcctcg ccgttcttta cgccgatcaa
     7801 gctgaccttt atggtgtcgc tgattctgtc agcgccggtg attctctatc aggtgtgggc
     7861 atttatcgcc ccagcgctgt ataagcatga acgtcgcctg gtggtgccgc tgctggtttc
     7921 cagctctctg ctgttttata tcggcatggc attcgcctac tttgtggtct ttccgctggc
     7981 atttggcttc cttgccaata ccgcgccgga aggggtgcag gtatccaccg acatcgccag
     8041 ctatttaagc ttcgttatgg cgctgtttat ggcgtttggt gtctcctttg aagtgccggt
     8101 ggcaattgtg ctgctgtgct ggatggggat tacctcgcca gaagacttac gcaaaaaacg
     8161 cccgtatgtg ctggttggtg cattcgttgt cgggatgttg ctgacgccgc cggatgtctt
     8221 ctcgcaaacg ctattggcga tcccgatgta ctgtctgttt gaaatcggtg tcttcttctc
     8281 acgcttttac gttggtaaag ggcgaaatcg ggaagaggaa aacgacgctg aagcagaaag
     8341 cgaaaaaact gaagaataaa ttcaaccgcc cgtcagggcg gttgtcatat ggagtacagg
     8401 atgtttgata tcggcgttaa tttgaccagt tcgcaatttg cgaaagaccg tgatgatgtt
     8461 gtagcgcgcg cttttgacgc gggagttaat gggctactca tcaccggcac taacctgcgt
     8521 gaaagccagc aggcgcaaaa gctggcgcgt cagtattcgt cctgttggtc aacggcgggc
     8581 gtacatcctc acgacagcag ccagtggcaa gctgcgactg aagaagcgat tattgagctg
     8641 gccgcgcagc cagaagtggt ggcgattggt gaatgtggtc tcgactttaa ccgcaacttt
     8701 tcgacgccgg aagagcagga acgcgctttt gttgcccagc tacgcattgc cgcagattta
     8761 aacatgccgg tatttatgca ctgtcgcgat gcccacgagc ggtttatgac attgctggag
     8821 ccgtggctgg ataaactgcc tggtgcggtt cttcattgct ttaccggcac acgcgaagag
     8881 atgcaggcgt gcgtggcgca tggaatttat atcggcatta ccggttgggt ttacgatgaa
     8941 cgacgcggac tggagctgcg ggaacttttg ccgttgattc cggcggaaaa attactgatc
     9001 gaaactgatg cgccgtatct gctccctcgc gatctcacgc caaagccatc atcccggcgc
     9061 aacgagccag cccatctgcc ccatattttg caacgtattg cgcactggcg tggagaagat
     9121 gccgcatggc tggctgccac cacggatgct aatgtcaaaa cacggtttgg gattgcgttt
     9181 tagagtttgc ggaactcggt gttcttcaca ctgtgcttaa tctctttatt aataagatta
     9241 agcaatagca tggagcgagc ctcaccatcg ggttcggtga aaatggcctg aaagccttcg
     9301 aacgcgcctt cggtaataat caccttatct cccggataag gggttgccgg atcgacaatg
     9361 tctttcggtt tatataccga tagctgatga atcaccgccg atgggactat cgctagcgac
     9421 gcgccaaagc gcacgaagtg gctgacaccg cgggtcgcgt tgatagtcgt ggtatgaatc
     9481 acttctggat caaattcgac aaacaggtag ttgggaaaca atggctcact gactgcagta
     9541 cgttttccac gcacgatttt ttccagggtg atcatcggtg ccaggcaatt cacagcctgt
     9601 ctctcaaggt gttcctgggc acgttgaagt tgcccgcgct tgcagtacag taaataccaa
     9661 gattgcataa tgactcttat ccgtttaatc ggggcgcaag gatagcaaaa gctttacgct
     9721 aagttaatta tattccccgg tttgcgttat accgtcagag ttcacgctaa tttaacaaat
     9781 ttacagcatc gcaaagatga acgccgtata atgggcgtag attaagaggc tacaatggac
     9841 gccatgaaat ataacgattt acgcgacttc ttgacgctgc ttgaacagca gggtgagcta
     9901 aaacgtatca cgctcccggt ggatccgcat ctggaaatca ctgaaattgc tgaccgcact
     9961 ttgcgtgccg gtgggcctgc gctgttgttc gaaaacccta aaggctactc aatgccggtg
    10021 ctgtgcaacc tgttcggtac gccaaagcgc gtggcgatgg gcatggggca ggaagatgtt
    10081 tcggcgctgc gtgaagttgg taaattattg gcgtttctga aagagcccga gccgccaaaa
    10141 ggtttccgcg acctgtttga taaactgccg cagtttaagc aagtattgaa catgccgaca
    10201 aagcggctgc gtggtgcgcc ctgccaacaa aaaatcgtct ctggcgatga cgtcgatctc
    10261 aatcgcattc ccattatgac ctgctggccg gaagatgccg cgccgctgat tacctggggg
    10321 ctgaccgtaa cgcgcggccc gcataaagag cggcagaatc tgggcattta tcgccagcag
    10381 ctgattggta aaaacaaact gattatgcgc tggctgtcgc atcgcggcgg cgcgctggat
    10441 tatcaggagt ggtgtgcggc gcatccgggc gaacgtttcc cggtttctgt ggcgctgggt
    10501 gccgatcccg ccacgattct cggtgcagtc acccccgttc cggatacact ttcagagtat
    10561 gcgtttgccg gattgctgcg cggtaccaag accgaagtgg tgaagtgtat ctccaatgac
    10621 cttgaagtgc ccgccagtgc ggagattgtg ctggaagggt atatcgaaca aggcgaaact
    10681 gcgccggaag ggccgtatgg cgaccacacc ggttactata acgaagtcga tagtttcccg
    10741 gtatttaccg tgacgcatat tacccagcgt gaagatgcga tttaccactc cacctatact
    10801 ggtcgtccgc cggatgaacc cgcggtgctg ggtgtcgcac tgaacgaagt gtttgtgccg
    10861 attctgcaaa aacagttccc ggaaattgtc gatttttacc tgccaccgga aggctgttct
    10921 tatcgtctgg cggtagtgac aatcaaaaaa cagtacgccg gacacgcgaa gcgcgtcatg
    10981 atgggcgtct ggtcgttctt acgccagttt atgtacacta aatttgtgat cgtttgcgat
    11041 gatgacgtca acgcacgcga ctggaacgat gtgatttggg cgattaccac ccgtatggac
    11101 ccggcgcggg atactgttct ggtagaaaat acgcctattg attatctgga ttttgcctcg
    11161 cctgtctccg ggctgggttc aaaaatgggg ctggatgcca cgaataaatg gccgggggaa
    11221 acccagcgtg aatggggacg tcccatcaaa aaagatcctg atgtcgtcgc gcatattgac
    11281 gccatctggg atgaactggc tatttttaac aacggtaaaa gcgcctgatg cgcgttagtt
    11341 ttgccctatt tatttgatcc gacagagaaa gcgcatgaca accttaagct gtaaagtgac
    11401 ctcggtagaa gctatcacgg atactgtata ccgtgtccga atcgtaccag acgcggcctt
    11461 ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca aacgtccgtt
    11521 ttcaatggct tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg gcgcttctga
    11581 aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagatcatc aaatcgtggt
    11641 cgatattccc cacggagaag cgtggctgcg cgatgatgaa gagcgtccga tgattttgat
    11701 tgcgggcggc accgggttct cctatgcgcg ctcgatttta ttgacggcgt tggcgcgtaa
    11761 tcccaatcgc gatatcacca tttactgggg tgggcgtgaa gagcaacatc tgtatgatct
    11821 ctgcgagctt gaggcgcttt cgctgaagca tcctggtctg caagtggtgc cggtggttga
    11881 acaaccggaa gcgggctggc gtgggcgtac tggcaccgtg ttaacggcgg tcttgcagga
    11941 tcatggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga tggcgaaaat
    12001 tgctcgtgac ctgttttgca gtgagcgtaa tgcgcgggaa gatcgtctgt ttggcgatgc
    12061 gtttgcattt atctgagata taaaaacccg cccctgacag gcgggaagaa cggcaactaa
    12121 aactttattc agtggcattt agatctatgt cgcatccggc aatcggtgca cgatgcctga
    12181 tgcgacgctg gcgcgtctta tcaggcctac attggtgcag gatcggtaga ccggataagg
    12241 cgttcacgcc gcatccggca agtggttaaa cccgctcaaa caccgtcgca ataccctgac
    12301 ccagaccgat acacatcgtc gccagaccaa actgaacgtc tttgtgttcc atcagattca
    12361 gcaacgtggt gctgatacgc gcaccggaac aacccagcgg atgacccagc gcgatcgcac
    12421 caccattgag gttgatcttc tcgtcaatct gctccattag cccgagatct ttaatgcacg
    12481 gcaggatctg cgcggcaaag gcttcgttca tctcaaacac gccgatatcg ctggcagaaa
    12541 gccccgcttt tttcagcgcc agcttcgagg caggaaccgg gccgtaaccc ataatcgacg
    12601 gatcacaacc aacgaccgcc atcgaacgca cacgggcgcg cggcttaaga cctaattcac
    12661 gggcgcggct ttcactcatc accagcatgg ccgcggcacc atcagaaagt gcagaagatg
    12721 tacccgccgt taccgtaccg cttactggat caaacgccgg acgcagcgtg gcgagggctt
    12781 ccacggtggt ttccgggcga atcacttcgt cgtaattaaa ctgcttcagg acgccgtcgg
    12841 catcgtgacc accggtcggg atgatttcat ttttaaatgc gcccgactgc gtggcggccc
    12901 aggcgcgggc gtgtgaccgc gcggcaaagg catcctgcat ttcacggctg ataccgtgca
    12961 tacgcgccag catttctgcc gttaagccca tcatgcccgc cgctttggcg acattgcggc
    13021 tcaggccggg gtgaaaatcg acgccgtgac tcatcggcac atggcccata tgctccacgc
    13081 cgccaaccag acctgcctgc gcatcgccag tcatgatcat tcgtgctgcg tcatgcagtg
    13141 cctgcatgga tgaaccacac aagcgattga cggtaaccgc cgggacagag tgcggcactt
    13201 ctgccagcag cgccgcgtta cgggcgatat taaaaccctg ctccagcgtc tgctgcacac
    13261 aaccccagta aatatcgtcg agggccgccg cttccagcgc cgggttacgc gccagcaggc
    13321 tacgcattaa atgagcggag agatcttctg cacgcacgtt acgaaaagca ccgcccttcg
    13381 aacggcccat cggggtgcga attgcatcga caatgacaac ctgttccatt gtgactcctt
    13441 aagccgtttt caggtcgcca accggacggg ctggctcaac cggaggatag tacggttcgt
    13501 tatgacgcgc tttattacgc agaccttccg gcacttcata cagcgggccg aggtgctgat
    13561 attgctgtgc catatcgagg tattttgcgc taccgagggt gtccagccag cggaacgcgc
    13621 cgccgtggaa cggagggaag cccaggccgt agaccagcgc catatccgct tccgccggag
    13681 tggcgataat gccctcttcc agacagcgca ccacttcgtt gaccatcggg atcatcatgc
    13741 gggcgataat ctcttcttcg ctgaaatcgc gcttcggctg gctcacttct gccagcaggt
    13801 cttcaacggc ggcgtcttct tctttcttcg gcttaccttt gctgtcttct ttataacgcc
    13861 agaaaccgag gccgttcttc tgaccaaagc ggttggcatc aaacagcgcg tcgatggcat
    13921 cgcggtaatc tttttgcatc cgctgcggga agcctgctgc catgacagcc tgagcgtgat
    13981 gcgcggtatc aatgcccaca acgtccagca gatatgccgg gcccatcggc cagccaaact
    14041 gtttttccat cactttgtcg atcttgcgga aatccgcgcc gtcgcgcagc agctggctga
    14101 aaccggcgaa atacgggaac agcacgcggt taacaaagaa gccggggcag tcgttaacca
    14161 caatcggcgt cttgcccatc ttgctcgccc aggcgacaac tttcgcgatg gtttcgtcgg
    14221 agcttttctc gccgcgaata atttctacca acggcattcg gtggaccggg ttaaagaagt
    14281 gcatcccgca gaagttttcc gggcgttcca gcgcgttggc cagttcgctg ataggaatgg
    14341 ttgaagtgtt agacgccagc acggtatcct ggcgtacttt ttgttcggtt tctgccagta
    14401 cggctttttt cactttcggg ttttcaacaa ccgcttctac cacaatatcc acgcggtcaa
    14461 atccggcgta gtcgagcgtt gggtggatcg tggagatcac gccagccagt ttcagaccat
    14521 cgatcttgcc gcgctcaagc tgcttgttca gcagtttcgc ggcttcggtc atgccgaggg
    14581 ttaacgactt gtcgttgata tctttcatga caaccggcac gcctttccac gcggactggt
    14641 aagcgatacc gccacccatg atgcctgcgc ccagcacagc agcctgtttc ggggtttcaa
    14701 cgtctttggt gagtttcttc gctttgcctt ttacatattg atcgttaagg aaaatgccga
    14761 ccagtgcgcg agcttcgttg gtatgcgcca gcgggacaaa acttttgttt tccagattta
    14821 aggcttcttc acgaccaaaa cgggccgcag cttcaatggt ttttactgcg gtgatggggg
    14881 ccggataatg tttccccgcc gtttgcgcga ccattccttt agcgatggtg aagctcatgg
    14941 tggcttcaat cttgctcagt tttagcggtt ccagcttcgg ctggcgtttc gctttccagt
    15001 cgaggtcgcc gttaatggcc tggcgtaaaa ccgcctttgc gccttcaacc agtttttctg
    15061 ctttgactac cccatccacc agaccgattt tcagcgcctg atccgcgccg acatctttac
    15121 cggcggcaat gatttccagc gcactgtcag cgcccagcat acgtggcata cgtacagaac
    15181 cgccaaagcc cggcatgatg cccagtttgg tttccggcag accgatgcgc agatccggcg
    15241 tcgccagacg ataatcggtc gccagcacgc attcgcagcc accacccagc gcatagccgt
    15301 tgacggcagc aatggtcggc accggcagat cttccaggcg attaaacacg ctattggcaa
    15361 agtgcagcca ctgacttaac tgttcttcag gaacgaggaa cagggacaaa aattcggtga
    15421 tatcagcacc gacgataaag gctgctttgt tcgaacgcag cagcagccct tttagatctg
    15481 attgctgttc cagcacgccg atggcctcgc cgaggctggc gacggtcgca gtgtcgagtt
    15541 tattaactga acctggggca tcaaatacca gttcggcaat gccatcttcc agccagtcaa
    15601 ggtacagggt gtcgcctttg taaagcatgt cagtctcctg aatccgcaag gtgatctggt
    15661 cgtaccagat gcatcgaagt gtgtattttg tgttaaaaat atgcaaataa aagattaaag
    15721 aaatgccgat ctgatcacgc tcggcagaaa tcacgctctg gatgaacgat gtgctaagat
    15781 gcggagactt aaggtcaaaa aaacagaagg gtaaaaaatg gaatcactgg cctcgctcta
    15841 taaaaatcat atagctacct tacaagaacg gactcgcgat gcgctggcgc gcttcaagct
    15901 ggatgcatta cttattcact ccggcgagct gttcaacgtt tttctcgacg atcatcccta
    15961 tccgtttaaa gtgaacccgc aattcaaagc gtgggtgccg gtaactcagg tgccaaactg
    16021 ctggttgctg gtggatggcg tgaacaagcc gaaactgtgg ttctatctgc cggttgatta
    16081 ctggcacaac gtcgaaccgc tgccgacctc cttctggact gaagatgtgg aagtgatcgc
    16141 actgccgaaa gccgatggca ttggtagcct gctgcccgct gcgcgcggca atatcggtta
    16201 tatcggtccg gtgccggaac gtgcgctgca actgggtatt gaggccagca acatcaaccc
    16261 gaaaggggtg atcgactacc tgcattacta ccgctccttc aaaaccgagt acgaactggc
    16321 ctgtatgcgt gaagcgcaga aaatggcggt caacggtcat cgtgcggcag aagaagcgtt
    16381 ccgttctggc atgagcgagt tcgatatcaa catcgcctat ctgaccgcga ccggtcatcg
    16441 tgataccgac gtaccttaca gcaacattgt ggcgcttaac gaacacgctt cggtgctgca
    16501 ttacaccaaa ctggatcatc aggcaccgga agagatgcgc agcttcctgc tggatgccgg
    16561 ggccgaatat aacggctatg cggctgacct gactcgtacc tggtcggcaa aaagcgacaa
    16621 cgactacgca cagctggtga aagacgtaaa tgatgaacaa ctggcgctga tcgccaccat
    16681 gaaagcaggc gttagctatg tggattacca catccagttc catcagcgca tcgccaaact
    16741 gctgcgtaaa catcaaatca tcaccgatat gagtgaagaa gcgatggtcg aaaacgatct
    16801 caccggaccg tttatgccgc atggtatcgg tcatccgctg ggcctgcagg tgcatgacgt
    16861 agccggtttt atgcaggatg atagcggtac acacctcgcg gcaccggcaa aatatccgta
    16921 cctgcgctgc acccgtattc tccagccggg tatggtgtta accatcgaac cgggtatcta
    16981 cttcattgaa tcgctactgg caccgtggcg tgaagggcag ttcagcaagc acttcaactg
    17041 gcagaaaatt gaagcactga aaccgttcgg cggcattcgt atcgaagaca acgtggtgat
    17101 ccacgaaaac aacgtggaaa acatgacccg ggatctgaaa ctggcgtgat ggaaagctgg
    17161 ttaattcctg cggcaccggt cacggtcgtt gaagagatca aaaaaagccg cttcataacg
    17221 atgttggcgc ataccgatgg cgttgaggcg gcgaaagcgt ttgttgaatc ggtgcgggca
    17281 gaacaccccg atgcccgcca ccattgcgtg gcgtgggtgg cgggtgcgcc ggatgattct
    17341 caacagctgg gtttctctga cgacggggag ccggcaggaa cggcaggtaa accgatgctc
    17401 gcccagctaa tgggcagcgg cgtcggggaa attacctctg tggtagtgcg ctactacggc
    17461 ggcatattgc ttggcaccgg tgggttagtg aaagcgtatg gcggcggcgt gaatcaggcg
    17521 ctgcgccagc taacgaccca acgcaagacg ccattaaccg aatatacttt gcaatgtgaa
    17581 tatcatcagt taaccggcat tgaagcgttg ctggggcagt gtgacggcaa aattatcaac
    17641 agtgattatc aggcattcgt tctgctgcgg gtggcgcttc cggcggcgaa agtggctgaa
    17701 ttttccgcaa agctggcgga ttttagccgt ggttcattgc aattgttagc gattgaagaa
    17761 taatccccac ttcgttttgc agaactaagg aagcggcaga gatgcatttt cgcgccatta
    17821 cccgaatcgt tggactactg gtcatcttat tttcagggac catgattatc cctgggctgg
    17881 tagcactcat ctaccgggat ggagcgggcc gcgcttttac ccagaccttt tttgtcgccc
    17941 tcgccattgg ttctatgctg tggtggccga accgtaaaga gaagggcgaa ctgaaatccc
    18001 gtgaggggtt tctgatagtg gtgctgttct ggaccgtgct gggtagtgtc ggtgcgctcc
    18061 cttttatctt ctcggaaagc ccgaacctca cgattaccga tgcgtttttt gaatctttct
    18121 ctggcctgac caccaccggg gccactacgc tggtggggct ggattcgctc cctcatgcca
    18181 tcctctttta tcgccagatg ctgcaatggt ttggcgggat ggggatcatc gtgttagcgg
    18241 ttgcgatact gcctatcctc ggcgtgggtg ggatgcagct ctatcgcgca gaaatgcccg
    18301 gcccgctgaa agataacaaa atgcgcccgc gaattgcgga aacggcgaaa accctgtggt
    18361 tgatttatgt cttgctgacc gtcgcctgtg cgctggcgtt gtggtttgcc ggaatggatg
    18421 cctttgatgc catcggccat agctttgcga ctatcgctat tggcggcttc tcgacacatg
    18481 atgccagtat cggttatttc gacagcccga ctattaacac tatcattgct atcttcctgc
    18541 tgatctccgg ctgtaactac ggtctgcact tttcactgtt aagtgggcgt agtctgaagg
    18601 tttattggcg cgatccggaa tttcgcatgt ttatcggcgt acagtttacg ctggtggtta
    18661 tttgtacact cgtactgtgg tttcataatg tctacagttc ggcgctgatg acaattaacc
    18721 aggcgttttt ccaggtggta tcgatggcga caaccgccgg gtttacgact gacagcattg
    18781 cccgctggcc gctctttttg ccggtactgc ttttatgttc agcttttatc ggcggttgcg
    18841 ccgggtcaac gggcggtggc ctgaaagtga tccgcatcct gctgctgttt aagcagggga
    18901 accgtgagct gaaacgactg gtgcatccga acgccgtcta tagcattaag ctggggaatc
    18961 gcgcactgcc ggaacgtatc ctcgaagccg tttggggatt tttctccgcc tatgcattgg
    19021 tgtttattgt cagtatgctg gcgattatcg ccacgggcgt ggatgacttt tctgcctttg
    19081 cttcagttgt tgcgacattg aataacctgg ggccagggct tggcgtggtt gctgataact
    19141 ttaccagtat gaacccggtg gctaaatgga tcctgattgc caacatgctg tttggtcgtc
    19201 tcgaggtctt tacattgctg gtgctcttta ccccgacttt ctggcgtgaa tgatggagta
    19261 atacgtgaaa acattaattc ttttctcaac aagggacgga caaacgcgcg agattgcctc
    19321 ctacctggct tcggaactga aagaactggg gatccagacg gatgtcgcca atgtgcaccg
    19381 cattgaagaa ccacaatggg aaaattatga ccgtgtggtc attggtgctt ctattcgcta
    19441 tggtcactac cactctgcgt tccaggaatt tgtcaaaaaa catgcgacgc gactgaattc
    19501 gatgccgagc gccttttatt ccgtaaacct ggtggcgcgc aaaccggaga agcgtactcc
    19561 acagaccaac agctacgcgc ggaagtttct gatgaactcg caatggcgtc ccgatcgctg
    19621 cgcggtcatt gccggggcgc tgcgttaccc acgttatcgc tggtacgatc gttttatgat
    19681 caagctaatt atgaagatgt caggcggtga aacggatacg cgcaaagaag ttgtctatac
    19741 cgattgggag caggtggcga atttcgcccg agaaatcgcc cagttaacca acaaaccgac
    19801 gctgaaataa gcataaagaa taaaaaatgc gcggtcagaa aattatttta aatttcctct
    19861 tgtcaggccg gaataactcc ctataatgcg ccaccactga cacggaacaa cggcaaacac
    19921 gccgccgggt cagcggggtt ctcctgagaa ttccgacaga gaaagcaaaa ataaatgctt
    19981 gactctgtag cgggaaagcg tattatgcac accccgcgcc gctgagaaaa agcgaagcgg
    20041 cactgctctt taacaattta tcagacaatc tgtgtgggca ctcgaagata cggattctta
    20101 acgtcgcaag acgaaaaatg aataccaagt ctcaagagtg
//
LOCUS       DAZPAC010000060        19989 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZPAC010000060 DAZPAC010000000
VERSION     DAZPAC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19989
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.060"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..69
                     /locus_tag="ACHUPM_001836"
     CDS             <1..69
                     /locus_tag="ACHUPM_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFR0869625.1"
                     /translation="ILKTTDYLRIAQPELLNFRRTA"
     gene            complement(70..174)
                     /gene="yoaI"
                     /locus_tag="ACHUPM_001837"
     CDS             complement(70..174)
                     /gene="yoaI"
                     /locus_tag="ACHUPM_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR0869626.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            344..790
                     /locus_tag="ACHUPM_001838"
     CDS             344..790
                     /locus_tag="ACHUPM_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR0869627.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(747..1568)
                     /gene="nimR"
                     /locus_tag="ACHUPM_001839"
     CDS             complement(747..1568)
                     /gene="nimR"
                     /locus_tag="ACHUPM_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFR0869628.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            1665..2846
                     /gene="nimT"
                     /locus_tag="ACHUPM_001840"
     CDS             1665..2846
                     /gene="nimT"
                     /locus_tag="ACHUPM_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFR0869629.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            2901..3248
                     /locus_tag="ACHUPM_001841"
     CDS             2901..3248
                     /locus_tag="ACHUPM_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR0869630.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3270..3524)
                     /gene="yoaF"
                     /locus_tag="ACHUPM_001842"
     CDS             complement(3270..3524)
                     /gene="yoaF"
                     /locus_tag="ACHUPM_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR0869631.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            3707..4732
                     /gene="dgcP"
                     /locus_tag="ACHUPM_001843"
     CDS             3707..4732
                     /gene="dgcP"
                     /locus_tag="ACHUPM_001843"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR0869632.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            4765..4863
                     /locus_tag="ACHUPM_001844"
     CDS             4765..4863
                     /locus_tag="ACHUPM_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR0869633.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            4866..4940
                     /gene="yoaJ"
                     /locus_tag="ACHUPM_001845"
     CDS             4866..4940
                     /gene="yoaJ"
                     /locus_tag="ACHUPM_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR0869634.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(4999..5247)
                     /locus_tag="ACHUPM_001846"
     CDS             complement(4999..5247)
                     /locus_tag="ACHUPM_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR0869635.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(5395..5577)
                     /locus_tag="ACHUPM_001847"
     CDS             complement(5395..5577)
                     /locus_tag="ACHUPM_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFR0869636.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(5581..5940)
                     /gene="yeaR"
                     /locus_tag="ACHUPM_001848"
     CDS             complement(5581..5940)
                     /gene="yeaR"
                     /locus_tag="ACHUPM_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFR0869637.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(6113..6751)
                     /gene="leuE"
                     /locus_tag="ACHUPM_001849"
     CDS             complement(6113..6751)
                     /gene="leuE"
                     /locus_tag="ACHUPM_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFR0869638.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(6878..7801)
                     /gene="dmlR"
                     /locus_tag="ACHUPM_001850"
     CDS             complement(6878..7801)
                     /gene="dmlR"
                     /locus_tag="ACHUPM_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFR0869639.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            7904..8989
                     /gene="dmlA"
                     /locus_tag="ACHUPM_001851"
     CDS             7904..8989
                     /gene="dmlA"
                     /locus_tag="ACHUPM_001851"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFR0869640.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVAIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            9240..10850
                     /gene="yeaV"
                     /locus_tag="ACHUPM_001852"
     CDS             9240..10850
                     /gene="yeaV"
                     /locus_tag="ACHUPM_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFR0869641.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGGAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            10882..12006
                     /gene="yeaW"
                     /locus_tag="ACHUPM_001853"
     CDS             10882..12006
                     /gene="yeaW"
                     /locus_tag="ACHUPM_001853"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFR0869642.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            12062..13027
                     /gene="yeaX"
                     /locus_tag="ACHUPM_001854"
     CDS             12062..13027
                     /gene="yeaX"
                     /locus_tag="ACHUPM_001854"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR0869643.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(13081..14196)
                     /gene="rnd"
                     /locus_tag="ACHUPM_001855"
     CDS             complement(13081..14196)
                     /gene="rnd"
                     /locus_tag="ACHUPM_001855"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR0869644.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(14278..15963)
                     /gene="fadD"
                     /locus_tag="ACHUPM_001856"
     CDS             complement(14278..15963)
                     /gene="fadD"
                     /locus_tag="ACHUPM_001856"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR0869645.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(16168..16749)
                     /gene="yeaY"
                     /locus_tag="ACHUPM_001857"
     CDS             complement(16168..16749)
                     /gene="yeaY"
                     /locus_tag="ACHUPM_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR0869646.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(16789..17484)
                     /gene="tsaB"
                     /locus_tag="ACHUPM_001858"
     CDS             complement(16789..17484)
                     /gene="tsaB"
                     /locus_tag="ACHUPM_001858"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR0869647.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(17542..19452)
                     /locus_tag="ACHUPM_001859"
     CDS             complement(17542..19452)
                     /locus_tag="ACHUPM_001859"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR0869648.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            19584..19928
                     /locus_tag="ACHUPM_001860"
     CDS             19584..19928
                     /locus_tag="ACHUPM_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0869649.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         4841 a         5187 c         4906 g         5055 t
ORIGIN      
        1 attttgaaaa ccacggatta tttgcgcatt gcgcaaccag aactgctcaa tttccgccgc
       61 actgcgtaac tagcctgtcc gttcaataag cagaacggac aaaaccagca caacagcaat
      121 ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac atttgatcgt tcatagcatg
      181 cccccgggag atagctgttt tcacgttatt gttagcgtgc acaaatggca gtttgatgac
      241 aattcgcctg attttttatg caaaaaacgt aaagattttc tacttccttc ctgcagcaaa
      301 cgtaaagtaa gcaggcttat tattttttgg caaggaaacc acgatgtttg atgtcacttt
      361 gctgatcctg ctcgggttag ctgcgctggg ctttatcagt cataacacca ctgtcgccgt
      421 ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta agcacctttt tcccgtggat
      481 tgaaaaacag gggctaagta tcgggataat cattctgacg attggcgtta tggcccccat
      541 cgccagcggg acgctaccgc cctcaacctt gattcactcg tttctgaact ggaaatcgct
      601 ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt gggcgcggcg tgaccttaat
      661 gggcagccag ccgcaactgg tcgccgggct gctggtaggc actgttttag gcgtggcgct
      721 gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt ctggtttcgc tgattgtggg
      781 gaaacagtag ttaatcccgc aatatatcgg cctggcgttt gccccaggcc tttcttaaac
      841 atagtaataa aggccgttgt tgaatcatac cccagcgtat gagcaacttt ctgtacagta
      901 tcgcctttca ctaatccctg aagtgccata atcagctgta actgctgacg ccactggcga
      961 aaactcaacc ccgtctcttt tactatcagt cgcgcaagat tgcgttcact catcgcaaaa
     1021 aagccagccc attgccctaa tgacccccac tcgacgggcc ctttcgccat catctccacc
     1081 atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca gctgttgttg tggctgctga
     1141 gggagttcat caaacaggac ctgaattaat ctgcgggtca tcggctcagc acgctgagtc
     1201 gtcgtcctgt ttgccagggt taaaatcaat tcccggcata agggcgatat tttcaatgtg
     1261 caacatgttg tcggcattgt cactgcgctg ggttcgataa acagaaaaca gagttcagcg
     1321 ttagctgtca cttgattgct atgttccacc ccgcccggta tccaaaccgc atattgcggc
     1381 ggcaccatcc ataaagcatt ttccaccgta caggtaattg caccatgtaa tgctagtatc
     1441 agctgtcctt tgcggtgctg atgtacagga ctggttaact catccgttcc ggcgtgaata
     1501 caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat agccattgag attcaacctg
     1561 tgcatcattt tgtccgaatt tagcgataat ttgtcatttt agcttgattc aacataacaa
     1621 taaaaacggt aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt
     1681 cattaagcgg caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat
     1741 tacgcgtcac ctttatcggc gcagcaccgt tactggatac gattcgttcc gcttactcgc
     1801 tgacgacagc gcaaaccggc ttattgacca ccctgccatt attggccttt gcgctaatct
     1861 cacctttggc caccccggta gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt
     1921 tacttttgat ctgtgctggt atcgcaattc gctctctccc ttcgccttac ttattatttg
     1981 gcggtacagc ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa
     2041 ttaaacgcga tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg
     2101 gagctgcagc ggcactggga tcggctatga tagtgccgct ggctttgaac ggttttggct
     2161 ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc
     2221 cacagtggcg aagccaacaa catgcaaatt tgagtacctc gcgcgcctta catactcggg
     2281 gtatctggcg ttcgccgctt gcctggcagg taacattgtt tcttgggatc aactcactga
     2341 tctattacgt gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag
     2401 cacaggcggg ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga
     2461 tccctctttt cttacatcat gtgaaagatc agcgtggtat tgcagcgttc gttgccttga
     2521 tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc gcacgcgatc acctggactc
     2581 tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc
     2641 gggcaagttc tgcgcatcag gcggcggcac tctcggggat ggcacaatcc gtcgggtatt
     2701 tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca cgatgctaac ggtgactggt
     2761 ccgtaccact gatgggtgtt gcaatacttt cactactgat ggcgattttc ggactttgcg
     2821 ccgggagaga caaagaaatt cgctaatatc cggtgctata gtgacgtgac aaatcatgcg
     2881 tgaaagggag aacaaacacg atgaatattc agtgcaaacg cgtttatgat ccggctgaac
     2941 agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc gcgcggtatc aaaaaaaccg
     3001 atttagccct tgatgagtgg gataaagaaa tcacgccgtc aacggaactg cgcaaagcct
     3061 ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca atatcttgca gaactggcgc
     3121 aacacgagca agaaggaaag cggctggcgg acatcgccaa aaaacagccg ctgaccctgc
     3181 tctacgcagc aaaaaacacc acgcagaacc atgcgctggt gctggccgac tggctacgta
     3241 gcttgtgatt ttagtacagc atccggcggt tatttttcac cagccggatg gtcccgtcgc
     3301 cacaatgccc attcatcaat cgtttcaccg cccggtaatt tgcaattgtt gctgacccct
     3361 tgcgctgtcg acactggaat gagcgtcccg cccttctgct ggcaatagac cgacgccgga
     3421 tttgccatac caatctgcgg cggtttaggt acttctggct gagaagaggt tgaacaacca
     3481 gccaggacca gcaagcaagg caaagcaaaa ctgataattt tcatttattg atctcactca
     3541 tttatctacg cttagagtat cgcggtatcg ttttgttttg cagcactatt tttattacat
     3601 tcactcaaaa catattacgt cttgtttcat ctttgttggt gatgttttat catgcctgca
     3661 aagattaaat aatcagcatt tacccgccgt atcctggagt tgtttcgtgt cagatcagat
     3721 tatcgcccgc gtctcgcaat cccttgccaa agaacagtca ctggaaagtc tggtccgaca
     3781 gcttctggag atgctggaaa tggtcactga tatggaatca acctacctga ccaaagtgga
     3841 tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac agccagaaaa tgcacatccc
     3901 ggagaatttt accgtctcgt gggattactc gttatgcaaa cgcgccattg atgaaaactg
     3961 ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt attgcggcac gcaatcttgg
     4021 catctccaca tttctgagca caccaattca cttaccggat ggatcattct atggcacgct
     4081 ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc gcggaacagg ttttacagtt
     4141 attcgccgga ctgattgcac aatatattca aaaagaggca ctggttgaac agttgcgcga
     4201 agccaatgct gcgctgattg cgcaatcgta taccgactcg ttaaccgggt tgccgaatcc
     4261 gcgggcgatt tttgaaaatc tgacgacgct gttttctctc gcccggcatc ttaaccataa
     4321 gataatgatc gcgtttatcg atctggataa cttcaaatta atcaatgatc gttttggtca
     4381 taatagtggc gatctgtttc tcattcaggt tggcgagcgc cttaatacgc tccagcaaaa
     4441 tggcgaagtt attggtcgtc tcggcggtga tgagtttttg gttgtttcac taaacaacga
     4501 gaatgcggat atttcgtcgc tgcgagaacg cattcagcag caaatacgtg gagaatatca
     4561 cttaggtgat gttgatttgt attatcccgg tgccagtctt ggcatagtag aagtcgatcc
     4621 tgaaacaacc gatgcagaca gtgccctgca tgctgccgat attgcgatgt atcaggagaa
     4681 aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg ccacattgct gaggcgtatt
     4741 cacatccttt tgattggtga taacatgcga atcggtatta tttttccggt tgtaatcttt
     4801 attacagcgg tcgtattttt agcatggttt tttattggcg gctatgctgc cccgggggca
     4861 taaagatgaa aaaaacaacg attattatga tgggtgtggc gattattgtc gtactcggca
     4921 ctgagctggg atggtggtaa cgtcacctct aaaaaatagc aaaggctgcc tgtgtgcagc
     4981 ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag ataaatagca cgacaatcgc
     5041 accaataacg gcaaccacga agctgccaaa attgaagcca tcgactttac caaagccaaa
     5101 cagcgtgctg atccatccgc cgactacggc accgactatc cccagcagga tagtcataaa
     5161 gaatccacca ccatctttac ctggcatgat ccacttcgcc agaataccgg caataagccc
     5221 aaaaataatc catgacagaa tgcccattgt ttcctcactt atctgttttg cattagcggg
     5281 ttagtcgctg ataaaaagca tagcacaaca tcgggagggc aagatttgtg acgagcatca
     5341 cggaggattt ttttgcgatg gcgcagaaat tgcgccatca acgatcagtg ataattacca
     5401 accacaaaca tcatgttcgt tttccgtgtc ataagaacgc acggtattca ccagatcttt
     5461 tatcacttca gccgccactt ctggcaccag caaagtcatc ggcgtctctg tttcataatc
     5521 gacagaaacg ccattactgt tattggtgac ggtcacggta tacgttgctt tgcccatgat
     5581 tcatttcccg ttatgaatga ctttccgttg ttgcgcacct tccatcagga cttcaggagc
     5641 cacgaagaag tcaatgttga aataagtatc gtcagtcatg gcttcaatgt tgtgccactt
     5701 ttctggaggg aacacctcaa actgccccgc ttcgataagg atcacctgat caggctctgc
     5761 actgtgttca tcagcgtagc cgagatattt gaccgcccca tgcataacgg aaaggcgtgg
     5821 gtaaaccccc gggcgcgttc ctttatcaag atgacgttcg aatattccgg caggcgcagt
     5881 ttgtttattc cagaaaggcg ttgagcgcgt atgaatataa ttctgtggga tttgcagcat
     5941 ccttttccct ccttcggtga atgcgctgaa aacggtttat tccagccgtt tcagggtacg
     6001 cctgataatt tgcattttaa ataccattta ttggttactt tttagcacca tatcagagaa
     6061 aaatcaggga ggattacaag tgggaagccc gcgacaacgc gggctgaatg tatcaggatt
     6121 gcagcgtcgc cagtcgggcg gcgaaaccca cgaacatcaa accaatcagt gagttgccaa
     6181 ctttagccag tttctttttg gtacgtatgt actgcgtgac aaaagcacca gaaataatca
     6241 ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt cgccagaata aagaatgaaa
     6301 ttcccgtatg tggggcatta acatcgataa actgtacgaa aaacgacaca tagaacaaaa
     6361 tggctttcgg attagtcagg ctcaaaatta acgcgcgttt aaaaatggcc ccgtattggg
     6421 gctcatcgga tttggcctcg ttatttttac ccttcagggt cgcgtagaga attttactcc
     6481 ccagatagag caaataaaac gcaccaagat aacgtacaat gttgaataat atcggggtgg
     6541 tcttaattaa tgtcgccact ccagcccatg ccagaaacat caatactgca tcgccaataa
     6601 atacaccgca ggccgcaaga taaccgcctt tcataccgct actgacgcta tttttgagta
     6661 caaacagggt atttggccct ggcaccaaca caataaaaat ggccccaacc agataggtcc
     6721 agtaattcag aaccccgtat tcagcgaaca cattaacctc tttaattatc tttcgatcat
     6781 gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc ttagaaagga gaaaatcagg
     6841 ttttaacccg atatcaaccc gataattgaa taattaatca ggcatgctcc agtgaaaaat
     6901 tcgggtagtg ctcggcaaaa tactggcgta aaaattctat cgttatccgc actttcgctg
     6961 acgtcgccag ccttgaaaca taaacggccc agacgttcgc tggctggtaa tattccggta
     7021 gcacttgcac taaatgacca ctggcaatgt tttcgctaac atcccaccag gagcgcagag
     7081 caatcccctg cccatcgaga caccactgat ggacaatttc tccatgatta gacgacagtg
     7141 ggccggtgac tttaatcgca tggtggcctt ctttattgcg taattgccag acaccaaaag
     7201 gatgatcgcg ctctttaatg accaggcacg gcaatgcgga aagatcggtt aaatgtttcg
     7261 gcgcgccatg ttgcgcaata aattccggcg acgcgcagag aatgcggtaa ttagtcgcca
     7321 gtttgcgggc gattaaatta ggggcgatgt catcgccaat gcgaatatcg agatcaacac
     7381 cttcattgac caaatcgacc aaccggtctt ccacatcaaa acgtaattcc agttgcggat
     7441 acgccttagc cagcgctgat aacgccggag ccaccacctg tcgtccaaaa ccaaagctgc
     7501 tgataatacg cagcatcccc tgcggcacct gacgcacgtc agaaagttcg tccatcatct
     7561 gaccgacatc ctgcaaaatc cgctgcgccc attcataaat ccgctctcct tcttcggtaa
     7621 tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt tagcgtttgc tccagcaagg
     7681 cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc ttccgccacg gcggcaaaac
     7741 cagcccggcg agccaccagc ataaagacgc gcaaatcgtt cagcagcggt aaattattca
     7801 tgattcgtgt tttatgtttc accagttacg gggattaatt ccttttcagt caattatagg
     7861 atggtgatgt tgtcaatttt gatggtcagg aagtgagaac ccaatgatga aaacgatgcg
     7921 tattgctgcg atcccgggag atgggattgg caaagaagtc cttcctgaag ggattcgcgt
     7981 attacaggct gccgctgagc gctggggctt cgacttgagt tttgagcaaa tggagtgggc
     8041 gagctgcgag tattacagcc atcacggtaa aatgatgccg gacgactggc atgagcaact
     8101 tagccgtttc gacgccatct attttggtgc cgtcggctgg ccggataccg ttccggacca
     8161 tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa ttcgaccagt acgtcaacct
     8221 gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg gcgggaaaac agcctggcga
     8281 catcgatttt tacgtggtca gggaaaacac cgaaggcgaa tattcctcgc tcggcggtag
     8341 agtgaatgaa ggtacagagc atgaagtcgc cattcaggaa tcggtattta cgcgtcgtgg
     8401 tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa agccgcccac gtaagacgct
     8461 aacttctgcc actaaatcaa acggtttagc catcagcatg ccgtactggg atgagcgagt
     8521 ggaagcaatg gccgagaatt acccggagat ccgctgggac aagcagcata ttgatattct
     8581 ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg gtggtggcgt ccaatttgtt
     8641 tggcgatatc ctttccgatc ttggcccggc ctgcaccggc accattggca ttgccccatc
     8701 cgccaacctg aatccggaac gcactttccc gtcgctcttc gagcctgtcc acggttccgc
     8761 gccggatatc tacgggaaaa atattgctaa ccctatcgcc acaatttggg ccggtgcgat
     8821 gatgctcgat tttctcggca atggcgatga gcgtttccag caagcgcata acggtattct
     8881 ggcagcgatt gaagaagtga ttgctcacgg gccgaaaaca ccggatatga aaggcagtgc
     8941 caccacgcca caggttgccg acgcgatttg caaaattatt ttgcgttaag gtcaaaccag
     9001 tttatttgaa ccgcgtcact gacgcggttt ttttattcgt tctttgcagt taataacctg
     9061 cgtcatttca ccttttattg tttccgtttc gtgttttatg gctttccgta ttcttaattg
     9121 tttaatttat gtaacatgca aataatcaat ttgttaaaca caacatacaa ctcattaccc
     9181 tgcaaataat tacaactggc aaaagccaga gacgggcttt tatgcccagg agatgactca
     9241 tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag catcagcctg gtggccattc
     9301 ttttcatcgc agctgcatta agccttttcc cacaacaatc ggccgacgcg gccaacacaa
     9361 tatacacttt tgttacgcgt acgttaggtt ccgccgtaca ggtattggtt ttgctggcaa
     9421 tgggactggt gatttattta gccaccagta aatacggcaa tattcgtctt ggcgaaggaa
     9481 aaccggaata cagcacgctc tcctggctgt ttatgtttat ttgtgccggt ttaggttctt
     9541 ctacgcttta ttgggggggt gctgaatggg cctattatta tcaaacgcct ggattaaata
     9601 tcgcaccacg ttcacaacag gcactcgaat ttagcgtccc ttactccttc ttccactggg
     9661 gcatcagcac ctgggcaact tatacgctgg cctcattaat catggcttat cactttcatg
     9721 tgcgtaaaaa caaaggtctg agtctttccg gcattattgc cgcgattacc ggcgttcgcc
     9781 cgcaaggccc atggggaaaa ctggttgatt tgatgtttct gatcgccact gtcggcgcac
     9841 tgactatttc ccttgttgtc accgcagcaa cctttactcg tggactttcc gcgctgaccg
     9901 gtttacccga taacttcacc gtgcaggcat ttgtgatcct gctttccggc ggcatttttt
     9961 gcctaagctc gtggattggt atcaacaacg gtttgcaacg tctgagcaaa atggttggct
    10021 ggggcgcgtt cctgctgcca ttactggtgc tgattgtcgg tccaaccgaa tttattacca
    10081 acagcatcat caatgccatc ggcctgacca cgcaaaactt cctgcaaatg agcttattca
    10141 ccgatccgct tggcgatggt tcatttaccc gcaactggac cgttttctac tggctgtggt
    10201 ggatctcata cccccctggc gtagcaatgt ttgtcacccg cgtttcccgc ggtcgtaaga
    10261 ttaaagaagt tatctgggga ctgatcctcg gcagcaccgt cggttgctgg ttcttcttcg
    10321 gcgtaatgga aagctatgcc attcatcagt ttatcaatgg cgtaatcaac gtcccacagg
    10381 tgctggaaac actgggcggc gaaacagctg tacagcaagt tctgatgtcg ttgccagccg
    10441 gtaaattgtt cctcgccgca tacctgggcg tgatgattat tttccttgcc tcgcatatgg
    10501 atgcagtggc ctacaccatg gcggcgacca gtacgcgtaa tctccaggaa ggtgacgatc
    10561 ctgaccgtgg gctgcgtctt ttctggtgcg tggtgatcac tctgatcccg ctttccatct
    10621 tgtttaccgg tgcttcgctg gaaacgatga aaaccaccgt cgtgctcaca gcccttccct
    10681 tcctcgtcat tttactggtg aaagtcggcg gatttattcg ctggctgaaa caggattatg
    10741 ccgacattcc ggctcatcaa gttgaacatt atctcccgca gacaccggtt gaagccctgg
    10801 aaaaaacacc agtgctccct gcgggaaccg tattcaaagg cgacaactga gcgccatcat
    10861 cctaacgata aaggtatccc tatgagcaat ctgagccctg actttgtact acccgaaaat
    10921 ttttgcgcta acccgcaaga ggcgtggacc attcctgccc gtttttatac cgatcagaac
    10981 gcgtttgaac acgaaaaaga gaacgtcttc gccaaaagct ggatttgcgt cgctcacagc
    11041 agcgaactgg cgaatgccaa tgattatgtg acgcgtgaga tcattggcga aagcatcgtg
    11101 ctggtacgcg gtcgtgataa ggttttgcgc gcgttctata acgtgtgtcc gcaccgtggt
    11161 catcagttgt tgagcggtga aggaaaagca aaaaatgtga ttacctgccc atatcacgca
    11221 tgggcattca aactcgatgg caatctggcc catgcacgta actgcgaaaa cgtcgccaat
    11281 ttcgatagcg acaaagcgca actggttccg gtgcgtctgg aggaatatgc cggattcgtc
    11341 ttcatcaaca tggaccccaa cgccaccagc gtagaagatc agttacccgg tctgggcgcg
    11401 aaagtgctgg aagcctgccc ggaagtccac gatctgaaac tggcggcccg ctttaccacc
    11461 cgcacgcctg ccaactggaa gaacattgtc gataactatc tcgagtgcta tcactgtggt
    11521 ccggcgcatc caggtttctc cgactccgta caggttgatc gctactggca caccatgcac
    11581 ggtaactgga cgctgcaata cggtttcgcc aaaccgtccg aacagtcgtt taaatttgaa
    11641 gagggtacgg atgcggcatt ccacggtttc tggctgtggc cgtgcacgat gctgaacgtc
    11701 accccgatca aagggatgat gacggtcatt tatgaattcc cggtggattc tgaaactacc
    11761 ctgcaaaact acgatattta cttcaccaat gaagagttaa ccgacgagca aaaatcgctg
    11821 attgagtggt atcgcgatgt gttccgtccg gaagatttac gtctggttga aagcgtacag
    11881 aaagggctga aatcgcgtgg ctatcgtggt caggggcgca tcatggccga cagtagcggt
    11941 agtggcattt ccgaacatgg tatcgcccat ttccataatc tgctggcgca ggtgtttaag
    12001 gactaatgac atcggcggcg gtattttccg ccgctgggct gatttttgat ggagtacagc
    12061 aatgtcagac tatcaaatgt ttgaagtaca ggtgagtcag gtggaaccgc ttaccgaaca
    12121 ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg ctgcctgcgt tcaccggagg
    12181 aagtcacatc attgtgcaga tgagcgatgg tgataaccag tacagcaatg cgtattcact
    12241 actgagttcg ccgcatgaca cctcttgtta tcagattgcc gttcggctgg aggaaaactc
    12301 gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg ggcgatcggt taacgatttc
    12361 aacgcctaat aacctgtttg cgctaattcc ctcagccaga aagcatctgt ttatcgcggg
    12421 cggtattggt atcacccctt tcctgtcgca catggcagag ttgcaacaca gcgacgtcga
    12481 ctggcagcta cattactgct cgcgaaatcc agaaagttgc gcatttcgtg atgagctagt
    12541 ccagcatccg caggctgaga aagtccattt gcatcattcg tcaaccggaa cacgactgga
    12601 attagcgcga ttattggcgg atatcgaacc tggcacacac gtttatacct gtggccccga
    12661 ggcgctaaat gaagcggtaa gaagtgaagc tgcgcgtctg gacatcgccg ccgatacgct
    12721 gcactttgag caatttgcta tcgaagacaa aaccggcgat gcctttaccc tggtgcttgc
    12781 ccgttccgga aaagagtttg tggtgccgga agagatgact attttgcagg ttattgaaaa
    12841 taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg gtatgcggaa cctgcgaaac
    12901 agcaatactg gaaggtgaag ctgaccatcg ggatcagtat tttagcgatg aagagcgtgc
    12961 cagccagcaa agtatgttga tctgttgttc gcgtgcgaag ggtaaacgcc tggtgttgga
    13021 tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc cagtccggct tcggaagatt
    13081 ttactgcgga tattcctgca ataaattgtg taatgcttcc gccatcagct caccacgcca
    13141 gccggaaatc agctccggca aattgttctg cggtttcagt ttccagtgcc agttcagcag
    13201 ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg atcttatgcg tttcgctcac
    13261 gtcagtaatc agcgacttaa tcgctttaaa cgctttacga taacccggca tgtccatcag
    13321 gttaagcatc ggctgcggta aggcctcttc cggcaatgtc tgcgcttttt ccaccagcgc
    13381 cagcagcgtt ttaccgtgaa agcggatttc gctaccggat aaacccaggc tgtccagttc
    13441 gcctaaactt cccggcatat aacgcgcgac cgaccacaaa tgctcttcac gcacgacaaa
    13501 gttcaccgcc agatcacgct cacgagcctt gcgcagtcgc cagtcggcta acagttgcag
    13561 acaggccagt tggcgtgtgc gtaattgcca ggcattggtg atatcacgcc aggcatcttc
    13621 cggcgcaacg acttcctgac gacgcatttg catcaggcgg cattcatcca gcgccgcagg
    13681 tagccagccg gaggcctccg tttctaccat aagcttggcg gtgatcggta acagatacca
    13741 gacatccgcc gctgcgtatt cacactgacg ttcggtcagc ggtctggcca gccagtcggt
    13801 gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct tccaccatgg aagcgaaacc
    13861 ccatgacatc gggcgtccgc agaaggcagc aaggatttgc gtgtcaatca ggggttgtgg
    13921 taattcgcca aagacattga ggaacacttc cagatcttca ctgcctgcat ggagaaattt
    13981 tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac cagtcggtga tcccgagagg
    14041 atcaattagc gccagatgct caccatcgaa aagttgaatc aaccccagct gcgggtaata
    14101 agtgcgcgta cgaacaaatt cagtatcctg ggctatcgcc ggaaagacac ggacggcttc
    14161 acacaaagaa gccagcgcat cgtccgtggt aatcatttgg taattcaaat tgtattctct
    14221 ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct gacgactgac ttaacgctca
    14281 ggctttattg tccactttgc cgcgcgcttc gtcacgtaat tctcgtcgca aaatttttcc
    14341 gacgttagat ttcggtaact catcacgaaa ctccaccagc ttcggtactt tgtagcccgt
    14401 gagctgacgg cagcaaaagg tcaccagtga ctcttcggta agcgatggat cttttttcac
    14461 tacgaagatt ttcaccgctt caccactgga gccggaaggt acgccaacag ccgcgacttc
    14521 ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg ttgggataga cgttaaaacc
    14581 ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc aggaaccctt cttcatccat
    14641 caccgcgatg tcgccggtgt gtaaccagcc atttttgatg atctcatcgg tagcatcggg
    14701 acgctgccag taacccagca tcacctgcgg tcctttgaca caaagctcac ccggttgacc
    14761 cggtggtact tcattatcgt cgtcatccac cagtttggct tccgtcgacg gcaccggcaa
    14821 accgatgcta ccactatgat aatcaatatc atatgggtta acgctgacca gcggcgcaca
    14881 ctcggtaaga ccatagcctt ccagcagata ctgtcctgtc agtttcaccc aacgctctgc
    14941 caccacttgc tgcactggca tcccaccgcc tgcggaaaga tgcagactgg agaaatccag
    15001 ctgctggaac tctttattgt tcagcaacgc attgaacaag gtgttaacgc cagtgatagc
    15061 ggtaaacgga tatttcgcta actcttttac caaccctgga atatcgcgcg ggttagtgat
    15121 aagcaggttc tgcccaccca gttcgataaa cagcaggcag ttaatggtca gggcaaaaat
    15181 gtgatacagc ggcagtgccg ttactaccag ttctttacct ggatgcagca acggcccata
    15241 ggtggcgttg acctgttcca ggttcgccag catattgcgg tgagtcagca tcgcgccttt
    15301 cgccacacct gtggtgccgc cggtgtattg caggaaggct aaatcttccg gcaccagttc
    15361 gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg ctacgaaatg aaatggcatc
    15421 tggcagatgg tatttcggca ccaaacgctt gatgtattta acaacgaaat tgactaccgt
    15481 gccttttgcc gtagatagct gatcgcccat acgggtcaga attacgtgct gaacggcggt
    15541 tttatcaacc actttttcca gtgtgtgagc aaagttagac acgataacaa tcgccgatgc
    15601 gccgctatcg ttaagctgat gctcaagctc acgcggggta tacaacgggt taacgtttac
    15661 gacgatcatc ccggcacgca aaatgccaaa cagcgccacc ggatattgca ataaattagg
    15721 catcatcaac gcaacgcgat cgcctttctt cagccccaac ccttgttgca aataagcggc
    15781 aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatt acctccccca tattcacaaa
    15841 cgcaggctga tcggcgtagc ggacgaccga ctgctcaaac atatctatca gagattgata
    15901 acggtcaggg ttgatctccg tcggaacatc cgcgggataa cggttaagcc aaaccttctt
    15961 caattcttca cctctaaaat gcgtgttcgt cgtcatcgca accccaagtg ataaacatgt
    16021 tgttaacata acattaactc atcttaccag cttgataatt agacaacgaa aaggttgcga
    16081 agcgcgtcac tatttatttt tatagttgcc gtatgaacgc agaaacagcg gaccagccgc
    16141 tgtttctttt tctttaaaaa caacttgtta ctcggttaca attgtttcaa ctcttgcagg
    16201 gccaggatta taccaacccc atccgccgta accatagggc cagccgcgtc caccataaaa
    16261 ccatggatca attggctgcg gcggcataat cacctgttga gtgatatgcc agcgtttgta
    16321 acctgttacc tgcataacca taaatttata tggtgcatta tttactttgc catccactgt
    16381 gccagtgatt ggcccgacaa ccgtcaccag ttgtccacga aaatcgaccg gatcaagaaa
    16441 accgttcaca tcggcataaa tgcgcccgcg tgaaggttct cccaaggttg gtcttgctcc
    16501 gctgtccagc ggtacggtag caatttccag gcgggttttc ccttgctggt tttgtaccac
    16561 aaccactttg ccgccaaagc gcgcctcctg accaacatat aaatctggcg cattcatcac
    16621 gcgtaccaga tcttgctgtg gtgttggact gctgccttta atggcgtccg gcacagtgac
    16681 acaaccgctc agcattagcg caaacgtacc tgccagtatg cctttgataa cattcttttg
    16741 aaccgccatg atgcgactcc tttttttctc agggcatact cttaagattc attctttgcc
    16801 cggaagtttc ttccatgcga cgttgttacg taaataaacc ggttcggcat gttccaccgc
    16861 caccgtttta ccctcggcaa acatctgaca cgcaatcggc agcatatctt ccgccgctgg
    16921 cagtaaaact tctccgtcgc gcaaaaccag cccgctctct ttacggagat ccggccaggc
    16981 ttgccagccc gtccctaccg ttacccattc gccggaaagc tgttgcattc gttcatgaac
    17041 gagttcgggt ttgagtacgg cttcggtttc ttcaccgcgc cagataccgt tttcatcacg
    17101 ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca atggctgcca gcacgcgggt
    17161 tgcgccgttt ttgcgccacg ccccctgcgc catcgtcatt agcgtagaga caccaatcat
    17221 cggtaactcc gcgccaagcg ccagcccttg cgcaatacca ataccaatgc gcacgccagt
    17281 aaagctaccg gggccgcgcc cgtaagccag agcgtttatg tcagtcaggg aagttccgct
    17341 ggtggtcagg atatcctgca ccatcggtaa gattcgttga gtatgttcac gagggcaaag
    17401 ctcaaaatga gcgttgacat taccgtcgtt ccacagagcg acagagcaga cctctgtcgc
    17461 ggtatcgata gccagaattc gcatgggtct tcgtgcttag atcaataaaa aggcgcgcat
    17521 cataccatac tccgtaacaa attacctgga ggatggtatc gcaaggaaac gaaccgcacg
    17581 ggcaatgtca cgggtgcgtg gcgcgggcgg cagactggcg agaaacgtcg cgccgtaagg
    17641 acgcatcacc agccgattgt cacaaattac cagcacgccg cgatcgtcgg cgtcgcgaat
    17701 cagtcgccct actccctgct tgagagtaat gaccgcatcc ggcagctgca cttcatcgaa
    17761 cgggtcgcca ccgcgcaaac gacaatcttc catgcgcgct tttaacagta gatcatccgg
    17821 cgaggtaaac ggcaatttgt cgataattac caatgacaat gtatcgccac gcacgtccac
    17881 cccttcccag aaactactgg ttgccacaag aagcgcgtta ccggcgctga caaattgctg
    17941 caacagttgc cctttgctgg tttccccctg caacaaaacg ggaagcgtca tggtagcgcg
    18001 gaactgctcg gccagatcgc gcatcatggc atgcgaggta caaagcataa aacaacgacc
    18061 gttgttagct tcgatgatcg gtcgcagcat tgccgccagt tgccgtgcac aacccggttg
    18121 attggtttgc ggcaggttgc gcggcacaca gagtaacgcc tggcggctgt aatcaaacgg
    18181 gcttggcaat agcaacgact ccgcctgttc tatcccaagc cgcgaggtga aatgatgcag
    18241 atcgtcgttc accgacagcg ttgctgaggt gaagatccag ctaccgggct tttgcgccat
    18301 taactctttg aatttatccg ccacgctgag cggcgtgaga gccagagtaa aatggcgcga
    18361 agtgcattcg taccagtagc tgtagcccgg ctgattgatc tcttttagcc gcttcagccg
    18421 tgtgcgatac aacgtggcgc gctcaaatgc cgcatccagc aaggcggaac gccccagtga
    18481 cagtttcgcc acgtcataac aaagttccag ggtgtcatcg agcagtaaaa atgcccgctg
    18541 aatttgcgga ttagctaaca gctcacgcag gttaccgcga taacctggct caccgagttg
    18601 cagacgaaaa tcctgtgcac tctgggcaag acgatcagcg cacttttgta actgctgggt
    18661 gtcttttaat tcggtgcggt aggcgatggt gatgtctttt gccaggtcga gcagttgtcg
    18721 actggagagt gactgaccaa aatactggct ggcaatgtcc ggtagctggt gggcttcgtc
    18781 gaagatcatg acgtccgctt cagggatcaa ctcgccaaat ccactctctt taaccaccat
    18841 atccgccaga aagagatgat ggtttaccac caccacatcg gcgtccatcg cttttttacg
    18901 tgctttgacc acaaagcaat ctttatacat cgggcagtcg ctgccaagac agttgtcgtt
    18961 ggtgctggtg accagcggcc acgcctgtga atcttccgcc acgctgacgc aggtgctgat
    19021 atcaccatcg actgtttgat tagaccagga gcgcagcagg atcacatcgc ttaagatttg
    19081 taccggcaga tcgccccccg ccagcgcctg ctgttcgaga cgttcgaggc agaggtagtt
    19141 tgagcgccct ttcagcagcg ccacgttgcc cgtatatttc aatgcctttg agactgttgg
    19201 caaatcgcgg ctgtagagct gatcctgcaa cgcttttgag ccggtcgaga taatgacttt
    19261 ctttttcgcc cgcagcgcag gagccaggta agcgtaggtt ttgcccgtac cggttcctgc
    19321 ttccaccacc agcggctggc ctttttctat cgcctgggtg acggctaccg ccatctgtcg
    19381 ctgtggttct cgcggcttaa agcctggtat cgctttcgcc agctgaccgt ctggtgcaaa
    19441 atcgtccgtc acactacccc ctgttgattt gaacagggat tatgtcagga tgagggcgct
    19501 ttcgccagtt gaagtggtga cggcaacctc acattgtggc agtctttgca gcacgaaaat
    19561 ggaagtataa tgaggacaaa atgatgacta tcgttcgtat cgatgctgaa gcccgctggt
    19621 ctgatgtagt aatccacaac aacacgctct actacactgg tgtaccggaa aacctcgacg
    19681 ccgatgcctt tgagcaaacc gccaacacgc tggcacagat tgacgccgtg ctggaaaaac
    19741 agggcagcaa taaatcgagc attctggatg ccaccatttt cctggccgat aaaaacgact
    19801 tcgcggcgat gaataaagcg tgggatgcct gggttgtcgc gggtcatgcg ccagtgcgct
    19861 gcacggtaca agcgggtttg atgaacccga agtataaagt tgaaattaag attgtggctg
    19921 cggtgtaagc tttatcgaag caaaataagt cagacgataa tttatcgata atactggtcg
    19981 gttttacat
//
LOCUS       DAZPAC010000061        19408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZPAC010000061 DAZPAC010000000
VERSION     DAZPAC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.061"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..245
                     /locus_tag="ACHUPM_001861"
     CDS             <1..245
                     /locus_tag="ACHUPM_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR0869650.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHUPM_001862"
     CDS             complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHUPM_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR0869651.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHUPM_001863"
     CDS             1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHUPM_001863"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFR0869652.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /gene="otnK"
                     /locus_tag="ACHUPM_001864"
     CDS             2164..3426
                     /gene="otnK"
                     /locus_tag="ACHUPM_001864"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFR0869653.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="ACHUPM_001865"
     CDS             3423..4061
                     /locus_tag="ACHUPM_001865"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR0869654.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="ACHUPM_001866"
     CDS             4066..4842
                     /locus_tag="ACHUPM_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR0869655.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="ACHUPM_001867"
     CDS             4931..6295
                     /locus_tag="ACHUPM_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFR0869656.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALCASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHUPM_001868"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHUPM_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR0869657.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHUPM_001869"
     CDS             complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHUPM_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR0869658.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHUPM_001870"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHUPM_001870"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR0869659.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHUPM_001871"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHUPM_001871"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR0869660.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHUPM_001872"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHUPM_001872"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR0869661.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHUPM_001873"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHUPM_001873"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR0869662.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHUPM_001874"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHUPM_001874"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR0869663.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHUPM_001875"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHUPM_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR0869664.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="ACHUPM_001876"
     CDS             complement(12844..13167)
                     /locus_tag="ACHUPM_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR0869665.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHUPM_001877"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHUPM_001877"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR0869666.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHUPM_001878"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHUPM_001878"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR0869667.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHUPM_001879"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHUPM_001879"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR0869668.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="ACHUPM_001880"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="ACHUPM_001880"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR0869669.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(17716..18009)
                     /gene="cas2e"
                     /locus_tag="ACHUPM_001881"
     CDS             complement(17716..18009)
                     /gene="cas2e"
                     /locus_tag="ACHUPM_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFR0869670.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="ACHUPM_001882"
                     /pseudo
     CDS             complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="ACHUPM_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
     gene            complement(18926..>19408)
                     /gene="cas6e"
                     /locus_tag="ACHUPM_001883"
     CDS             complement(18926..>19408)
                     /gene="cas6e"
                     /locus_tag="ACHUPM_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR0869671.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         4592 a         5230 c         4848 g         4738 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggca cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgctct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt atgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgctcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgtgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgagca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca agttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgctcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggaa gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacaca ctaagcatac ataactgttt
    17641 ttaaacaaat ttattccgca tcaacaatct accaactaaa ttaaaacatt tccttatttt
    17701 taaagaacgg ataacttatt gattatcaac aggaagaaaa gaaaccaaac gtaacccatc
    17761 caaatccacc ggaatacgtc tgttttctcc ccaggtctga aattcaaaac ccgactcgat
    17821 attggtcgcc caggccatca ccacatttcc gcaaccagcc agttgggtaa tttgctgcca
    17881 gatcatctcc cgaatacgtt ttgatgtatc accaacatac acaccggcac gcacttccag
    17941 tagccagatt gcgagccgtc cacgtaagcg cggagggaca ttttctgtaa caaccacgac
    18001 catgctcatc cgccgcgccc ccggtgacca ctatcaccca gcgtttcagg ttcagggatg
    18061 gcaggcggta acatatccgg cgcgggttgt ggtggttcaa tttcacctgc agcaaggact
    18121 tcctcaatta acggtattaa tttgcccgtt aacttagtgc tacggaaaat atcgcgacag
    18181 gctaatctga cttctttatc aggttctgcg ggttgcctcg ctgctatttc aaatgccttt
    18241 ggcacaaccg aatcaaattt aatgatatcg gctatgtcat aaacaaatga aagcggtttg
    18301 ccactatgga taaacccaat agcgggcgca tatcccgcgg ctaatactgc cgcttcagat
    18361 ataccgtaca gacatgatgt agcagcactg atgcagcgat tcacaacatc gcctttttcc
    18421 cagtctttag gatcgtattt gcgaccattc catttcacac catattgttt cgccagtaat
    18481 gcatacgtct ggcgaacgcg agatccctca attccacgta actgctccac tgaacggcga
    18541 gctggcggtg gttcacgaaa acgtaattca tacattttgc gcaccacctt caaccgtaga
    18601 tcttccgtta aagccagctt tgcctagtag agtaatttat ctgcccgcgc ccctccgggt
    18661 tgtccggagg agtaaacgcg aacgcccgct tcaccgaccc agaccagcag tgttcccacc
    18721 gtggcggcca gatggactgc cgcgtgggaa actcttgttc ccggttcgag cataatgcag
    18781 gcgaccgatc ccactggaat atgcgtgcgg atcccggttt tgtcgatcag cacgaaagcg
    18841 ccgtccagta cgtcgatttg accgtactgg aggaagatca tggaagtgcg atcttttaat
    18901 gggatcgggc tcagcggtac aaacgtcacg cctctgctcc gggtttgatc agcatcagac
    18961 cacaaccgaa cgcccggctt ttgccatacc cctgactgag acgttgcaga aataacccag
    19021 gagcggtgac cgtaagcatc cccgtataat ccacgctact gaactggatc agttgccggg
    19081 agttttcccg ccgcagttgc tgttgtcggt aggcatcaac cgaagtatcc agcagtgtaa
    19141 aaccgcttct ctccccctgc gctgccagcc agtccagcgc agcctgttgt tgatgcaacc
    19201 agacatcact tccttccacc tgccccctca cctgccgttt cgcctccatc agcaagtcgt
    19261 ggcgtttgcc cgctttacaa attgttggat tcgcccgcag gttgaaacaa agggattgtc
    19321 cggtacgcaa ctctggcgca aatggtcggc attcgatggt gaaaatatca ctttccgccg
    19381 ggcgttcctg cgacaggaca aaaaagcg
//
LOCUS       DAZPAC010000062        18919 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZPAC010000062 DAZPAC010000000
VERSION     DAZPAC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.062"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(150..2141)
                     /gene="tkt"
                     /locus_tag="ACHUPM_001884"
     CDS             complement(150..2141)
                     /gene="tkt"
                     /locus_tag="ACHUPM_001884"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR0869672.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2419..3177
                     /gene="loiP"
                     /locus_tag="ACHUPM_001885"
                     /gene_synonym="yggG"
     CDS             2419..3177
                     /gene="loiP"
                     /locus_tag="ACHUPM_001885"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR0869673.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3320..4240)
                     /gene="speB"
                     /locus_tag="ACHUPM_001886"
     CDS             complement(3320..4240)
                     /gene="speB"
                     /locus_tag="ACHUPM_001886"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR0869674.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4378..6354)
                     /gene="speA"
                     /locus_tag="ACHUPM_001887"
     CDS             complement(4378..6354)
                     /gene="speA"
                     /locus_tag="ACHUPM_001887"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR0869675.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(6363..6494)
                     /gene="yqgB"
                     /locus_tag="ACHUPM_001888"
     CDS             complement(6363..6494)
                     /gene="yqgB"
                     /locus_tag="ACHUPM_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR0869676.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(6588..6731)
                     /gene="yqgG"
                     /locus_tag="ACHUPM_001889"
     CDS             complement(6588..6731)
                     /gene="yqgG"
                     /locus_tag="ACHUPM_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR0869677.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(6842..7093)
                     /gene="yqgD"
                     /locus_tag="ACHUPM_001890"
     CDS             complement(6842..7093)
                     /gene="yqgD"
                     /locus_tag="ACHUPM_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR0869678.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            7149..8303
                     /gene="metK"
                     /locus_tag="ACHUPM_001891"
     CDS             7149..8303
                     /gene="metK"
                     /locus_tag="ACHUPM_001891"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR0869679.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            8739..10133
                     /gene="galP"
                     /locus_tag="ACHUPM_001892"
     CDS             8739..10133
                     /gene="galP"
                     /locus_tag="ACHUPM_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR0869680.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            10210..10707
                     /locus_tag="ACHUPM_001893"
     CDS             10210..10707
                     /locus_tag="ACHUPM_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR0869681.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            10802..11509
                     /gene="endA"
                     /locus_tag="ACHUPM_001894"
     CDS             10802..11509
                     /gene="endA"
                     /locus_tag="ACHUPM_001894"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR0869682.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            11589..12320
                     /gene="rsmE"
                     /locus_tag="ACHUPM_001895"
     CDS             11589..12320
                     /gene="rsmE"
                     /locus_tag="ACHUPM_001895"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR0869683.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            12333..13283
                     /gene="gshB"
                     /locus_tag="ACHUPM_001896"
     CDS             12333..13283
                     /gene="gshB"
                     /locus_tag="ACHUPM_001896"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR0869684.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            13392..13955
                     /locus_tag="ACHUPM_001897"
     CDS             13392..13955
                     /locus_tag="ACHUPM_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR0869685.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            13955..14371
                     /gene="ruvX"
                     /locus_tag="ACHUPM_001898"
     CDS             13955..14371
                     /gene="ruvX"
                     /locus_tag="ACHUPM_001898"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR0869686.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(14546..15526)
                     /locus_tag="ACHUPM_001899"
     CDS             complement(14546..15526)
                     /locus_tag="ACHUPM_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR0869687.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            15544..16248
                     /gene="yggS"
                     /locus_tag="ACHUPM_001900"
     CDS             15544..16248
                     /gene="yggS"
                     /locus_tag="ACHUPM_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR0869688.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16266..16832
                     /gene="yggT"
                     /locus_tag="ACHUPM_001901"
     CDS             16266..16832
                     /gene="yggT"
                     /locus_tag="ACHUPM_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR0869689.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            16829..17119
                     /gene="yggU"
                     /locus_tag="ACHUPM_001902"
     CDS             16829..17119
                     /gene="yggU"
                     /locus_tag="ACHUPM_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR0869690.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIVALIN"
     gene            17127..17720
                     /locus_tag="ACHUPM_001903"
     CDS             17127..17720
                     /locus_tag="ACHUPM_001903"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR0869691.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            17713..18849
                     /gene="hemW"
                     /locus_tag="ACHUPM_001904"
     CDS             17713..18849
                     /gene="hemW"
                     /locus_tag="ACHUPM_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR0869692.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         4513 a         5029 c         4933 g         4444 t
ORIGIN      
        1 gcatatcgtg atgcatatta cataattgat atttattgca tataaatatt tgtgatctac
       61 aacacgcttt atctattgct tgtccgcaaa cggacatatc aaggtaataa aaaaggtcgc
      121 cgaagcgacc tttttacccg aaatgctaat tacagcagtt cttttgcttt cgcaacgacg
      181 ttgtcgacgg tgaagccgaa ctcttcgaac agcaactctg ccggagctga ctcaccgaag
      241 gtggtcatac cgatgatagc gccattcagg ccaacatact tgtaccagta gtcagcaata
      301 cccgcttcta ccgcaacgcg tgcagtaacc gctttcggca gtacggattc acggtaagcc
      361 gcatcctgct tgtcgaatgc atcggtagac ggcatggaaa ccacgcgcgc cttcacgcct
      421 tcggcagtca gtttttcgta ggcggcaaca gccagttcaa cttccgaacc ggtcgcgatg
      481 aagatcagtt ccggctgacc agcacagtct ttcagcacat aaccaccgcg cgcgatgttt
      541 gccagttgct cttcagtacg ttcctgctgc gccagattct gacgggagag gataagcgcg
      601 gtcggaccgt cctgacgctc aacaccgtat ttccacgcga ccgcggattc aacctggtca
      661 cacggacgcc atgtagacat gttcggggtc acgcgcagag aagcgacctg ctcaaccggc
      721 tggtgagtcg ggccgtcttc gcccagaccg atggagtcgt gggtgtaaac catcacctga
      781 cgctgtttca tcagcgcagc catacgtaca gcgttacgtg cgtattcgac aaacatcagg
      841 aaggtggagg tgtacggcag gaagccaccg tgcagggaga taccgttagc aatcgcggtc
      901 ataccgaact cgcgaacacc gtagtggatg tagttacccg cagcatcttc gttgattgct
      961 ttagaaccag accacagggt caggttagac ggtgccaggt cagcggagcc gccgaggaat
     1021 tccggcaaca gcgggccgaa cgcttcaata gcattctgag acgctttacg gctggcgatt
     1081 ttcgccggat tagcctgcag tttagcgata aactctttcg ctttggcgtc gaagtcagac
     1141 ggcatttcgc ctttcatacg gcgggtaaat tcagccgctt cctgcggata agctttcgcg
     1201 taagccgcaa acttctcatt ccatgcagat tctttcgcct ggcctgcttc tttcgcatcc
     1261 cactgcgcat agatttcaga cgggatttcg aacggtgcgt atttccagcc cagctgttcg
     1321 cgggtcagag cgatttctgc atcacccagc ggcgcaccgt gggagtcgtg agtaccggct
     1381 ttgttcgggg aaccgaaacc gatgatggtt ttgcacatca gcagggacgg tttgtcagtc
     1441 actgcgcgcg cttcttctac tgcgcgtttg atggatgctg cgtcatgacc gtcgatgtcg
     1501 cgaataacgt gccagccgta agcttcgaaa cgcattgcgg tgtcgtcggt gaaccagcct
     1561 tcaacgtgac catcaataga gataccgttg tcatcgtaga acgcaatcag tttacccagc
     1621 ttcagcgtac ccgccagaga gcaaacttcg tgggagatgc cttccatcat gcagccgtcg
     1681 cccatgaagg cgtaggtgta gtggtcgaca atgtcgtggc ccggacggtt aaactgcgcc
     1741 gccagcgttt tttctgcaat cgccataccg actgcgttgg caataccctg acccagcgga
     1801 ccggtggtgg tttccacacc agcggtataa cctacttccg ggtggcccgg agttttagag
     1861 tgcagctgac ggaagttttt cagttcttcc atcggcagat cgtaaccggt gaggtgcagc
     1921 aggctgtaga tcagcatgga gccgtggccg ttggacagca cgaagcggtc acggtcagcc
     1981 caggacggat tctgcgggtt gtgtttcagg aaatcacgcc acaggacttc ggcaatgtca
     2041 gccataccca taggggcacc cgggtgaccg gatttggctt tctgtactgc gtccatgctc
     2101 agcgcacgaa tagcattggc aagctcttta cgtgaggaca ttttgactcc agatcggatg
     2161 atgaagggca cgcccttaac gacttgacga cagcgcgttt tgggctacgc cggaaaattt
     2221 gccaacaatt taccgcaagc cgcgcgtcat gtacatggaa catccttttg ccgcttcaga
     2281 aatctctgga tcatgctcgc atgttgcgca atctactcgc ccgtccgctg cgcttttcct
     2341 tatactgaga ctgagcgtcg attcacctgc aaacggcgca tttttagaat aatcctgacc
     2401 ttgtgcggaa gagaaaacat gaaaattcgc gccttattgg tagcaatgag cgtggcaacg
     2461 gtactgactg gttgccagaa tatggactcc aacggactgc tctcatcagg agcggaagct
     2521 tttcaggctt acagtttgag tgatgcgcag gtgaaaaccc tgagcgatca ggcatgtcag
     2581 gagatggaca gcaaggcgac gattgcgcca gccaatagcg aatacgctaa acgtctgaca
     2641 actattgcca atgcgctagg caacaatatc aacggtcagc cggtgaatta caaagtgtat
     2701 atggcgaagg atgtgaacgc ctttgcaatg gctaacggct gtatccgcgt ctatagcggg
     2761 ctgatggata tgatgacaga taacgaagtc gaagcggtga tcggtcacga gatggggcac
     2821 gtggcgttag gccatgtgaa gaaagggatg caggtggcgc ttggtacaaa tgccgtgcga
     2881 gtagctgcgg cctctgcggg cgggattgtc ggcagtttat ctcaatcaca acttggtgat
     2941 ctgggcgaga aattagtcaa ttcgcaattc tcccagcgcc aggaagcaga agccgatgat
     3001 tattcttacg atcttctgcg ccaacgcggc atcagcccgg caggtcttgc caccagcttt
     3061 gaaaaactgg cgaaactgga agaaggccgc caaagctcaa tgtttgacga ccatccggca
     3121 tccgccgaac gcgcccagca tattcgcgat cgcatgagcg cggatgggat taagtaaagc
     3181 ctggtggtgt cgaacgtagg tcagataagg cgttcacgcc gcatctgaca tcaatggcac
     3241 atttgttacc ctgtgcgcat tgccggatgc gatgctgact gcatcttatc cggcctacgg
     3301 gtggcatcgc atctggtgct tactcgccct ttttcgccgc ttggatatac agcatctcca
     3361 gcgccagcgt cgccgcagcc agagcggtga tttccgactg atcgtatgcc ggagccactt
     3421 ccactacgtc catcccaaca atgttgagat ctttcaggcc gcgtaccagt ttaatagcgc
     3481 gatcggaggt caggccgcca atcactggcg taccggtgcc tggtgcaaaa gcaggatcca
     3541 ggcagtcgat atcaaaagtc aggtaaaccg gcatatcacc cacaatctgt ttcacttggg
     3601 cgataacgtc atccacgctg cgatcgttca cctggcaggc gtccagcacg gtaaagccgt
     3661 tgtctttatc aaactcggta cgaataccaa tctgcacgga atgattcggg tcgatcagac
     3721 cttctttcgg cgcggtatag aacatagtgc cgtggtcaaa ttcacaaccg ttcgcatagg
     3781 tatcggtgtg ggcgtcaaag tgtaccagcg ccattttgcc gaaatgcttc gcatgagcac
     3841 gcagcagcgg cagcgtaaca aagtggtcac caccgaaggt gagcatacgc ttaccggcag
     3901 ccagcagctt ctcggcgtgc gcctgcagct tttcgctcat ctcacgggca tcgccaaagg
     3961 catataccag atcgccgcag tccacgacgt tcagacgctc acgcatgtcg aaattccacg
     4021 ggaagcggtt gtgttcccag gccagattcg tcgaaacctg acggatcgct gccggaccgt
     4081 ggcgaccacc cgcacgacca gaagtggcca tatcgaacgg cacgccagta atcacccagt
     4141 ctgcatcgct gtcatacggc tggaagttca tcggcaggcg taaaaaacca aaggcattgg
     4201 aaaccagtga gttatcgtat tgatgaccta aggtgctcat ggatggactc ctattacaaa
     4261 gtcgatataa aaaaaccctt ccgcgtcgtt aggcccgacg aggaagggtt ggatttgtca
     4321 caataaattg tggcggatta tcaccgctaa attaagcgga ttcaagtaac acaggactta
     4381 ctcatcttca agataagtat aaccgtacaa acctgcctcg aactcttcaa ggaactgttg
     4441 ttgcagttca gcatcaagat cggttttctt cacttgatcg cggaactggg ttaacagcgt
     4501 tttcggatcg agctgtacat attgcagcat gtccgccacg gtatcgcctt cgtcagacag
     4561 ttctacttct acgctaccgt cagggaacac gaacacgtca accgcttcgg tatcaccgaa
     4621 caggttgtgc atattgccaa ggatctcctg atatgcgccg accataaaga aaccgagcat
     4681 cggcggattc tctggatcgt actccggcat tggcattgtc gtggcaatac catcaccatc
     4741 aatatagtgg tcgatagcac cgtcagagtc acaggtaata tccagcagca cagcgcgacg
     4801 ttccggcact tgatccagcc cttccagcgg cagaaccggg aacaactggt cgatccccca
     4861 tgcgtccggc attgactgga acagcgagaa attgacgtac attttgtccg ccatacgttc
     4921 ctgcagttcg tcgattatcg gacgatgcgc acggttttgc ggatccagtt gcttctgcac
     4981 ttcatggcac atgctcagat agagttgttc tgcccatgca cgttcttgca ggctaaaggt
     5041 gccggaagag tagccgatat gaatgtcgtg cagatccatc tggctgtcgt gtaaccattc
     5101 acgcaaagaa cggcgagttc ccggttcgtg catttcctgc caggtttccc acatgctttg
     5161 cagcgcgcgc ggcgcatctt ctgcgggcgc ggtcggcacc gtatattcgt tacgttccac
     5221 gccgatgata ttcgacacca gcacggtatg gtgcgcagtc accgcacggc ccgattcggt
     5281 gattaccgtc ggatgcggca gaccattttc ttcacacgca tcgccaatcg cccagatgat
     5341 gttgttggcg tattcattga ggccatagtt tacggagcag tcagactgcg aacgggtgcc
     5401 ttcatagtcc acgcccagac cgccgccgac gtcgaagcac tgaatattga cgcccagctt
     5461 gtgcagttcc acatagaaac gcgcggattc acgaacgccc gtggcgatat cgcgaatatt
     5521 cgccatctgc gaaccgaggt ggaagtgcag tagttgcagg ctgtcgagac gcccggcttc
     5581 acgcagggtt tcaaccagtt gcagtacctg agtcgcagcc aggccgaact tcgatttttc
     5641 cccgccggag gactgccatt tacccgagcc ctgcgaagcc agacgtgcac gcacgcccag
     5701 acgaggaacg acattcagac gttctgcttc atccagcaca atggcgattt ctgacatctt
     5761 ctcaatgacc agatagacct tgtgccccat cttctcgcca attaatgcca ggcggatata
     5821 ttcgcggtct ttataaccgt tgcagacgat gacgctacgg gtcatgccag catgtgccag
     5881 tactgccatc aactcggctt tggaaccggc ttccagaccc agcggttcgc ccgaatgaat
     5941 cagggactca atcacgcggc ggtgctggtt aactttgatc ggataaacaa ggaagtaatc
     6001 gccgttatag ccgtaggatt ccctcgcacg tttgaacgcg gcgttaatgg aacgcaaacg
     6061 gtgctgcagg atctgtggga aacagaacag tgcaggcaga cgctgaccct gtgcttcacg
     6121 agttttcact aactgcgcga gatcgacgcg agcttccggg acgtccgggt ccgggcacac
     6181 gctgatgtgg cccagctcgt taacgtcata gtagttattg ccccaccagg caatattgta
     6241 agtacgcagc atcttgctgg cttcctggga gctcattgca acctcctgca tggagcgtag
     6301 tacaccgtgt tcgcccgctg acgaaggcaa acccatagac atgtcgtcag acatagcgaa
     6361 cctcaaatta ttttattaag tgtaaaacag ttaacgacta tcgcagcctg gaactgcgat
     6421 aacaacccat aagcacatgg attttccagc agtgaatgct gacgctccaa ctgcgcaacc
     6481 ggtttctttt tcataacatt attaagcaca taaccgaacg taagtgtgac agttcggcga
     6541 aaccacgaga aaactcttgt ttttacaaga gcgcccttgt tcagtcctca gtaactgtaa
     6601 ccagctcttg aatcctgaga agcgccgaga tgggtataac atcggcaggt atgcaaagca
     6661 gagatgcaga gtgcggggaa cgaatcttca ccagaacggt gagacaggtt aagcagcaga
     6721 caacggttca ttatttcgta tcacctccac ggccgcctgt taagacgaac ccacaagcca
     6781 aaactctgat ttcaacccgg ctggaagtgg caacacgaaa gaaacgtcgt gtgcttttta
     6841 tttaagccgc gcgccgcgtt ttatacccca caatggcaga aaattgcaaa agataaatac
     6901 gcagaatgcc ggcattgtca ggaaaaattt ccagccacgt ttttaacaga atgagacacg
     6961 attcaaaaaa aagtggaaat cgggtgaaga attgacctaa aatagccatc cagatgttaa
     7021 tccatccata ccgattaaca ctcagactgc cagtgttttt aacctgcaga gtcgtggtag
     7081 gatccgctac cacagaaaat ccacacaaca gtttgagcta accaaattct ctttaggtga
     7141 tattaaatat ggcaaaacac ctttttacgt ccgagtccgt ctctgaaggg catcctgaca
     7201 aaattgctga ccaaatctct gatgccgttt tagacgcgat cctcgaacag gatccgaaag
     7261 cacgcgttgc ttgcgaaacc tacgtaaaaa ccggcatggt tttagttggc ggcgaaatca
     7321 ccaccagcgc ctgggtagac atcgaagaga tcacccgtaa caccgttcgc gaaattggct
     7381 atgtgcattc cgacatgggc tttgacgcta actcctgtgc ggttctgagc gctatcggca
     7441 aacagtctcc tgacatcaac cagggcgttg accgtgccga tccgctggaa cagggcgcgg
     7501 gtgaccaggg tctgatgttt ggctacgcaa ctaatgaaac cgacgtgctg atgccagcac
     7561 ctatcaccta tgcacaccgt ctggtacagc gtcaggctga agtgcgtaaa aacggcactc
     7621 tgccgtggct gcgcccggac gcgaaaagcc aggtgacttt ccagtatgac gacggcaaaa
     7681 tcgttggtat cgatgctgtc gtgctttcca ctcagcactc tgaagagatc gaccagaaat
     7741 cgctgcaaga agcggtaatg gaagagatca tcaagccgat tctgcccgct gaatggctga
     7801 cttctgccac caaattcttc atcaacccga ccggtcgttt tgttatcggt ggcccgatgg
     7861 gtgactgcgg tctgactggt cgtaaaatta tcgttgatac ctacggcggc atggcacgtc
     7921 acggtggcgg agcattctct ggtaaagatc catcaaaagt ggaccgttcc gcagcctacg
     7981 cagcacgtta tgtcgcgaaa aacatcgttg ctgctggcct ggccgatcgt tgtgaaattc
     8041 aggtttccta cgcaatcggc gtggctgaac cgacctccat tatggtagaa actttcggta
     8101 ctgagaaagt gccttctgaa caactgactc tgctggtacg tgagttcttc gacctgcgcc
     8161 catacggtct gattcagatg ctggatctgc tgcacccgat ctacaaagaa accgcagcat
     8221 acggtcactt tggtcgtgaa catttcccgt gggaaaaaac cgacaaagcg cagctgctgc
     8281 gcgatgctgc cggtctgaag taatctttct tcacctgctt tcaaaggcca gcctcgcgct
     8341 ggcctttttc ttttacctca ccgcggtgga taggcattca cgccgcatcc ggcaaaaagc
     8401 tgcccgcaca ataacatcat tcttcctcaa cacgttttat cgcaaattat catcactact
     8461 gaaaccgatt acaccaataa caccagccaa atatttgtaa tattttcata ttattattca
     8521 gttttcacag ttgttacatt tcttttcagt aaagtcttaa ttgcagataa cagcgtttaa
     8581 tctatgatga tataactcaa ttattttcat gcacttaaac cataactaag ataaatgtta
     8641 gtgtaagcga ttacactgat gtgatttgct tcacatcttt ttacgtcgta ctcacctatc
     8701 ttaattcaca ataaaaaata accatattgg agggcatcat gcctgacgct aaaaaacagg
     8761 ggcggtcaaa caaggcaatg acgtttttcg tctgcttcct tgccgctctg gcgggattac
     8821 tctttggcct ggatatcggt gtaattgctg gcgcactgcc gtttattgca gatgaattcc
     8881 agattacttc gcacacgcaa gaatgggtcg taagctccat gatgttcggt gcggcagtcg
     8941 gtgcggtggg cagcggctgg ctctccttta aactcgggcg caaaaagagc ctgatgatcg
     9001 gcgcaatttt gtttgttgcc ggttcgctgt tctctgcggc tgcgccaaac gttgaagtac
     9061 tgattctttc ccgcgttcta ctggggctgg cggtgggtgt ggcctcttat accgcaccgc
     9121 tgtacctctc tgaaattgcg ccggaaaaaa ttcgtggcag tatgatctcg atgtatcagt
     9181 tgatgatcac tatcgggatc ctcggtgctt atctttctga taccgccttc agctacaccg
     9241 gtgcatggcg ctggatgctg ggtgtgatta tcatcccggc aattttgctg ctgattggtg
     9301 tcttcttcct gccagacagc ccacgttggt ttgccgccaa acgccgtttt gttgatgccg
     9361 aacgcgtgct gctacgcctg cgtgacacca gcgcggaagc gaaacgcgaa ctggatgaaa
     9421 tccgtgaaag tttgcaggtt aaacagagtg gctgggcgct gtttaaagag aacagcaact
     9481 tccgccgcgc ggtgttcctt ggcgtactgt tgcaggtaat gcagcaattc accgggatga
     9541 acgtcatcat gtattacgcg ccgaaaatct tcgaactggc gggttatacc aacaccacag
     9601 agcaaatgtg ggggacagtg attgtcggcc tgaccaacgt acttgccacc tttatcgcaa
     9661 tcggccttgt tgaccgctgg ggacgaaaac caacgctaac gctgggcttc ctggtgatgg
     9721 ctgctggcat gggcgtactc ggtacaatga tgcatatcgg tatccactct ccgtcggcgc
     9781 agtatttcgc catcgccatg ctgctgatgt ttattgtcgg ttttgccatg agtgccggtc
     9841 cgctgatttg ggtactgtgc tccgaaattc agccgctgaa aggccgcgat tttggcatca
     9901 cctgctccac tgccaccaac tggattgcca acatgatcgt tggcgcaacg ttcctgacca
     9961 tgctcaacac gctgggtaac gccaacacct tctgggtgta tgcggctctg aacgtactgt
    10021 ttatcctgct gacattgtgg ctggtaccgg aaaccaaaca cgtttcgctg gaacatattg
    10081 aacgtaacct gatgaaaggt cgtaaactgc gcgaaatcgg cgctcacgat taatctcctc
    10141 aggcttcctc ccgtcgcgga ggaagccgcc tcttgcagtc atcttttctt cgctctatcc
    10201 tctgccgcta tgaaaacatc ccgtctccct atcgccatcc aacaggccgt tatgcgcggc
    10261 ctgcgggaaa aactcgccca ggccaacctg aagctagggc gtaactaccc ggagccaaaa
    10321 ctctcttaca cccagcgcgg aacctccgcc ggaacagcct ggctggaaag ctatgaaatc
    10381 cgcctcaatc ccgttttgct gttggaaaac agtgaagctt ttattgaaga agtggtaccg
    10441 cacgaactgg cacatttgct ggtatggaaa catttcggcc gcgtagcgcc acatggcaaa
    10501 gagtggaagt ggatgatgga aagcgtgctg ggtgttcccg cccgtcgtac gcatcagttc
    10561 gaactgcaat ccgtgcgtcg caacaccttc ccctaccgct gcaagtgcca ggagcatcag
    10621 cttaccgtac gccgccataa tcgcgtagtt cgtggcgagg ccgtctatcg ctgtgttcac
    10681 tgcggtgaac agctggttgc gaaataacca tctgaactat caggaacttt cctgatctgg
    10741 ctgattgcat accaaaacaa ctttcgctac gttgctggct cgttttaaca cggagtaagt
    10801 gatgtaccgt tatttgtcta ttgctgcggt ggtactgagc gcagcatttt ccggcccggc
    10861 gttggccgaa ggtatcaata gtttttctca ggcgaaagcc gcggcggtaa aagtccacgc
    10921 tgacgcgccc ggtacgtttt attgcggatg taaaattaac tggcagggca aaaaaggcgt
    10981 tgttgatctg caatcgtgcg gctatcaggt gcgcaaaaat gaaaaccgag ccagccgcgt
    11041 agagtgggaa cacgtcgttc ccgcctggca gttcggtcac cagcgccagt gctggcagga
    11101 cggtggacgt aaaaactgcg ctaaagatcc ggtctatcgc aagatggaaa gcgatatgta
    11161 taacctgcag ccgtcagtcg gtgaggtgaa tggcgatcgc ggcaacttta tgtacagcca
    11221 gtggaatggc ggcgaaggcc agtacggtca atgcgccatg aaggtcgatt tcaaagaaaa
    11281 agctgccgaa ccaccagcgc gtgcacgcgg tgccattgcg cgcacctact tctatatgcg
    11341 cgaccaatac aacctgacac tctctcgcca gcaaacgcag ctgttcaacg catggaacaa
    11401 gatgtatccg gttaccgact gggagtgcga gcgcgatgaa cgcatcgcga aggtgcaagg
    11461 caatcataac ccgtatgtgc aacgcgcttg ccaggcgcga aagagctaac ctacactagc
    11521 gggattcttt ttgttaaccc ctaccccacg cgtacaaccg cgtggggaga cgacgcggat
    11581 ttttaactat gcgtatcccc cgcatttatc atcctgaacc actgaccagc cattctcaca
    11641 tcgcgctttg cgaagatgcc gccaaccata tcgggcgcgt actgcgcatg gggccggggc
    11701 aggcgttgca attgtttgac ggtagcaacc aggtctttga cgccgaaatt accagcgcca
    11761 gcaaaaaaag cgtggaagtg aaggtgctgg aaggcaagat cgacgatcgc gaatctccgc
    11821 tgcatattca cctcggtcag gtgatgtcgc gtggtgaaaa aatggaattt acgatccaga
    11881 aatcgatcga actcggtgta agcctcatta cgccactttt ttctgagcgc tgcggcgtta
    11941 aactggatag tgaacgtctg aacaagaagc tccagcagtg gcagaagatt gcaattgctg
    12001 cctgtgagca gtgtggtcgt aaccgggtgc cggaaatccg tccagcgatg gatctggaag
    12061 cctggtgtgc agagcaggat gaaggactga aactgaatct tcacccgcgc gccagtaaca
    12121 gcatcaatac gttgccgtta ccggttgaac gcgtccgcct gctgattggc ccggaaggcg
    12181 gtttatcggc agatgaaatt gccatgactg cccgctatca atttactgat atcctgttgg
    12241 gacctcgcgt tttgcgtaca gagacaactg cgctcaccgc cattaccgcg ctacaagtac
    12301 gatttggcga tttgggctaa cggagaagaa taatgatcaa gctcggcatc gtgatggacc
    12361 ccatcgcaaa catcaacatc aagaaagatt ccagtttcgc tatgttgctg gaagcacagc
    12421 gtcgtggtta cgaacttcac tatatggaga tgggcgatct gtatctgatc aatggcgaag
    12481 cccgcgccca tacccgcacg ctgaacgtga agcagaacta cgaagagtgg ttttcgttcg
    12541 tcggtgaaca ggatctgccg ctggccgatc tcgatgtgat cctgatgcgt aaagacccgc
    12601 cgtttgatac cgagtttatc tacgcgacct atattctgga acgtgccgaa gagaaaggga
    12661 cgctgatcgt taacaagccg cagagcctgc gcgactgtaa cgagaaactg tttaccgcct
    12721 ggttctctga cttaacgcca gaaacgctgg ttacgcgcaa taaagcgcag ctaaaagcgt
    12781 tctgggagaa acacagcgac atcattctta agccgctgga cggtatgggc ggcgcgtcga
    12841 ttttccgcgt gaaagaaggc gatccaaacc tcggcgtgat tgccgaaacc ctgactgaga
    12901 atggcactcg ctactgcatg gcacaaaatt acctgccagc cattaaagat ggcgacaaac
    12961 gcgtgctggt ggtggatggc gagccggtac cgtactgcct ggcgcgtatt ccgcaggggg
    13021 gcgaaacccg tggcaatctg gctgccggtg gtcgcggtga acctcgtccg ctgacggaaa
    13081 gtgactggaa aatcgcccgt cagatcgggc cgacgctgaa agaaaaaggg ctgatttttg
    13141 ttggtctgga tatcatcggc gaccgtctga ctgaaattaa cgtcaccagc ccaacctgta
    13201 ttcgtgagat tgaagcagag tttccggtgt cgatcaccgg aatgttaatg gatgccatcg
    13261 aagcacgttt acagcagcag taacccacct tagcgagaag gatctcgttg agactctgag
    13321 tgacagcgcc cttctttcca cgcatactgg gcgctgttgc ttttttgaac caggaaacag
    13381 aacctctgac aatgaattta cagcatcact ttcttattgc catgcctgct ctccaggatc
    13441 cgattttccg tcgttccgtg gtctacattt gcgaacataa taccaatggt gcaatgggga
    13501 tcatcgtcaa caagccgctg gaaaatctca aaattgaagg gattctggaa aagctgaaga
    13561 tcacgccgga gccgcgtgat gaatcaatcc gtctggataa accggttatg ctcggcggtc
    13621 cgctggctga agatcgcggg tttattttgc atactccgcc ctccaatttt gcttccagca
    13681 ttcgcatttc agacaacaca gtgatgacca cttcccgcga tgtgctggaa acgctcggca
    13741 ccgataaaca accgtctgac gtattggtgg ctctaggtta tgcctcctgg gaaaaaggtc
    13801 aactggaaca agaaattctc gataacgcgt ggctaacggc cccggcggat ctgaatattc
    13861 tgttcaaaac gccgattgcc gaccgctggc gcgaggcggc aaaactgatt ggtgtggata
    13921 ttctcaccat gcctggtgtg gcagggcacg cctgatgagt ggaaccttac tcgccttcga
    13981 cttcggcacc aaaagcattg gcgtagcggt cggccaacgc attaccggca ctgctcgccc
    14041 tttgcctgca attaaagcac aggacggtac gccggactgg aacctcatcg aacgtttact
    14101 gaaagagtgg cagccggacg aaatcatcgt cggtttgccg ctgaatatgg acggcaccga
    14161 gcaaccattg actgccagag cgcgtaaatt tgccaaccgt attcatggtc gtttcggtgt
    14221 tgaagtaaag ctccatgacg aacgtcttag caccgtggaa gcccgttccg gtctgtttga
    14281 acagggcggc tatcgggcgc tcaacaaagg caaagtcgac tctgcctctg cggttattat
    14341 tctcgaaagc tatttcgagc agggatatta aggcgattta aaagcctggc ggatagtcgt
    14401 taccgccatc ttgccggatg cggcgtgaac gcctatccgg cctacatgtg gcagcgattg
    14461 taggtctgat aagacgcgca aacgtctcat cagacgttta ttgcctttgt aggcccgata
    14521 agcttgcaca tcgggcatgg caacgtcaca aacgcccttc ccccactcgc tgctgaaaac
    14581 tctgctgaaa cgttatcatc cccacctgct gcccggtttg aataacatgc ggtaactggt
    14641 gggttttccc ttcgcgaatc aaattcccca ccgcgggagt gttaatcaat aattcaaaca
    14701 gcgccacgcg tccttcctgt ttatccactt ccagcttttg tgacaacacg gcccgtaaac
    14761 tacctgccag ttgattacgt acgggatctt tttcctgcgc cggaaatgaa tccaccagtc
    14821 gctcaactgc ctgcgcggca ccacgcgtat gtaatgttgc cagcaccaaa tgcccggttt
    14881 ctgccgccgt cagtgccaga cggattgtct cgctgtcacg cagctctccg agcaaaatca
    14941 catcaggatc ttcccgcaat gcggcccgca atcccgatgc gaacgtcata cagtgcaaac
    15001 caatttcccg cttctggatc aaacatcgct ggctggcata gatatattca acaggatctt
    15061 ccagcgtcag aatatgcgca tcggcatgtt gattgagata gccaaccatc gccgccagcg
    15121 tggtagattt gctgctcccc gtcgcccccg tcaccagaat caggccattc tcactcttga
    15181 gtaattccgg caataccgtt ggtgcgccaa gctgttcgag ctgcgggcag tgcgaaggta
    15241 acagccgtaa cgccagcgaa atgccatgcc gttgcgcaaa tgcgctgcca cgcaatcgct
    15301 ggttttccgc cagcgacacg gcaaaatcca gctggccatt ttccagcaat attgcccgct
    15361 gatcgtcatc cagccactcc cgcagtagct cttcgacgtc cggcgcctca aacggcgcag
    15421 cttccattag cccgcgaatg cgccatcgtg cgggccaggc gctgcacagg tgtagatccg
    15481 agacgttatg ctttacacta agggccacaa tttcttccat attcatacta agatcctcgg
    15541 aaaatgaacg atattgcgca taacctggca caggtccggg acaaaatctc agcggctgca
    15601 acgcgttgcg gccgttctcc agaagaaatt acgctgcttg cagtcagtaa aacaaaacct
    15661 gcgagcgcca tcgcagaagc cattgatgcc gggcagcgtc aatttggtga aaactacgtt
    15721 caggaagggg tagataaaat tcgccacttt caggaactgg gcgtaacagg attagaatgg
    15781 cattttattg gcccgttgca gtctaataaa agccgcctgg tggcagagca tttcgactgg
    15841 tgtcatacca tcgaccgttt gcgcattgct acccgcctca acgatcagcg cccggcagaa
    15901 cttccccctc ttaacgtact gattcaaatt aatattagtg atgaaaacag taagtccggg
    15961 attcaactgg cagaactgga cgagctggca gcggcggtcg ctgaactacc gcgtttacgt
    16021 ctgcgcgggc tgatggcaat ccctgcgcct gagtcagaat atgtaaggca gtttgaagtt
    16081 gcacgccaaa tggctgtagc atttgccgga ctgaaaacgc gctacccgca tatcgacacg
    16141 ctctctctgg gaatgtcgga cgatatggaa gccgccattg cggcaggtag cacgatggtt
    16201 cgtatcggca ctgcaatttt tggtgcgcgt gattactcta aaaaataagg aattaaagga
    16261 acgccatgaa tacgttgact ttcctgcttt caacggtcat tgagctgtat accatggtgc
    16321 tgttattacg catctggatg cagtgggctc attgtgattt ttacaacccc ttctcacagt
    16381 ttgtagtgaa ggtaacgcag ccaattatcg ggccactgcg ccgcgttatt ccggcaatgg
    16441 gccccattga cagcgcctcg ctgctggttg cctatattct cagttttatc aaagccatcg
    16501 tgctgtttaa agtggtgacc ttcctgccaa tcatctggat tgctggttta ctgattctgc
    16561 tgaaaaccat cggcctgctg attttctggg tcctgctggt gatggcgatt atgagctggg
    16621 taagccaggg tcgtagtccg attgaatacg tgctgattca gctggccgat ccgctgctgc
    16681 gcccgattcg ccgcctgcta ccggcaatgg gtgggattga tttctcgccg atgatcctcg
    16741 ttctgctgct gtatgtcatc aatatgggtg tcgcagaagt attacaggca accggaaaca
    16801 tgctgctgcc ggggctgtgg atggcgttat gagtgccgta acagttaatg atgacggtct
    16861 ggttttacgg ctctatattc agccgaaagc cagccgtgat tctattgtcg gtttacatgg
    16921 cgacgaagtt aaagtcgcca ttaccgcgcc gccggttgac ggccagggca acagtcatct
    16981 ggtgaagttt ctcggtaagc aattccgggt tgccaaaagc caggtggtga ttgaaaaagg
    17041 cgaacttggc cgccacaaac aaattaaaat cattaatccg caacaaatcc cgccagaaat
    17101 cgtggcgtta attaattagg tatcctatgc aaaaagttgt cctcgcaacc ggcaatgccg
    17161 gtaaagtgcg tgagctggcg tcgctgctta gcgacttcgg tcttgatatc gtggcccaaa
    17221 cagacctcgg cgtagattcc gctgaagaaa ccggcctgac ctttatcgaa aacgcgattc
    17281 tgaaagcgcg ctatgcggca aaagtgaccg gtttaccggc aattgccgac gactctggtc
    17341 tggcggtaga tgcgctgggt ggcgcgccgg gtatttactc cgcgcgttat tccggtgaag
    17401 acgcgaccga tcaaaagaat ctgcaaaaac tgttggaaac actgaaagac gtaccggacg
    17461 accaacgtca ggcgcgtttc cactgcgtgc tggtatatct gcgtcacgcg gaagatccga
    17521 ctccgctggt atgccacggc agctggccgg gcgtgattac tcgcgaacca gcgggcactg
    17581 gtggctttgg ttatgatcca atcttcttcg taccttccga agggaaaacc gccgccgaac
    17641 tgacccgcga agaaaagagc gccatttccc accgtggtca ggcgttgaaa ctgctgctgg
    17701 acgctttacg taatggttaa attaccgccg ctgagtctct acattcacat cccgtggtgc
    17761 gtgcagaaat gcccgtactg cgatttcaac tctcacgcgt tgaaaggaga agtgccgcac
    17821 gacgattatg ttcagcatct gctttgcgat ctggacaatg atgtggctta cgctcagggc
    17881 cgtgaagtaa agacaatctt tattggcggt ggtacgccga gcctgctttc cggcccggcg
    17941 atgcaaacgc tgctggacgg cgtgcgtgcg cgtttgccgc tggcagcgga tgcagaaatt
    18001 actatggaag cgaaccctgg aacggtagaa gccgatcgct ttgtcgatta tcagcgtgct
    18061 ggtgtgaacc gcatctctat tggtgtgcag agttttagcg aagaaaagct gaaacgactt
    18121 gggcgtattc atggcccgca agaagcgaaa cgcgcggcaa agctggcgag cgggctgggg
    18181 ctgcgtagct ttaacctcga tttgatgcat ggactaccgg atcaatcact ggaagaggcg
    18241 cttggcgatc tacgccaggc cattgaactg aatccgccgc atctttcctg gtatcaactg
    18301 accatcgaac ccaatacgct gtttggttcg cgaccaccgg tgctgccgga cgatgacgcg
    18361 ctgtgggata ttttcgaaca ggggcatcag ttattaaccg cagcgggtta tcagcaatat
    18421 gaaacttccg cttacgccaa acccggttat cagtgccagc acaatctcaa ctactggcga
    18481 tttggtgact acatcggtat tggctgcggc gcgcacggca aagtcacctt cccggatggg
    18541 cgcattctgc gtaccactaa aacgcgtcat cctcgtggtt ttatgcaagg aaggtatctg
    18601 gaaagccagc gtgatgtcga agccgcagat aagccgtttg agttctttat gaatcgcttc
    18661 cgtctgctgg aggccgcgcc gcgcgtggag tttattgcgt ataccgggct ttgcgaagat
    18721 gtgattcgcc cacagttaga cgaggcgatt gcccagggtt atctcaccga atgtgcggat
    18781 tactggcaga taaccgagca cggtaaatta ttcttaaact cacttcttga attattcctc
    18841 gctgagtaat cattaatgcg cctgcgggcg cattaatcac ttctttcccc cccgttaatt
    18901 aacttcggca aaatatatt
//
LOCUS       DAZPAC010000063        18523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZPAC010000063 DAZPAC010000000
VERSION     DAZPAC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.063"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1710
                     /locus_tag="ACHUPM_001905"
     CDS             <1..1710
                     /locus_tag="ACHUPM_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFR0869693.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="ACHUPM_001906"
     CDS             1711..3360
                     /locus_tag="ACHUPM_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR0869694.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="ACHUPM_001907"
     CDS             3365..4141
                     /locus_tag="ACHUPM_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR0869695.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHUPM_001908"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHUPM_001908"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFR0869696.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="ACHUPM_001909"
     CDS             complement(5430..6752)
                     /locus_tag="ACHUPM_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFR0869697.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHUPM_001910"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHUPM_001910"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFR0869698.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHUPM_001911"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHUPM_001911"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFR0869699.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHUPM_001912"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHUPM_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFR0869700.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="ACHUPM_001913"
     CDS             complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="ACHUPM_001913"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFR0869701.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11632..14481
                     /gene="rcsC"
                     /locus_tag="ACHUPM_001914"
     CDS             11632..14481
                     /gene="rcsC"
                     /locus_tag="ACHUPM_001914"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR0869702.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="ACHUPM_001915"
     CDS             complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="ACHUPM_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR0869703.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="ACHUPM_001916"
     CDS             complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="ACHUPM_001916"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR0869704.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4526 a         4535 c         4867 g         4595 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga
     9721 atatcaccct gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct
     9781 gaggctttgg tggtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca
     9841 cagccgttgt cctctatcga aatggcctgt tgtgagtcgc tgtattgcca ggtttgaatg
     9901 cgaattttcc ctcgtgcgct gatagcctgg acggcattga tcaggatatt cagtagtacc
     9961 tgtttgagca gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg
    10021 aagtcgaccc gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat
    10081 gcattgaggc tgacttgttg ccattgactg tggcgtggac gtgaaaattc gagcaattgc
    10141 tgaataactt tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt
    10201 attgggtcac tggtttgttg gcgcaagatc tgtacataac cacgaatagc cgttaacgga
    10261 ttacgtactt catgcgcgac gccagccatc agctcaccca gtgtggcgag gcgttctgct
    10321 tgcgccatgc ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca
    10381 cctatcattt caccgtgcgt gttatgaata cgactggtag tgacactgag ttcaatggtg
    10441 cggtcacgac ctggaaaact gatctccagc gccacatgtt cggtgccatg ttccagcgta
    10501 tccagtactg gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc
    10561 agttcatggc gttgatagcc agtgataact tctgctgctg ggttcatggt ggttacatca
    10621 ccctggcggt caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt
    10681 gtccgcgttt cacgcagtgc ctgggcgagg ttattaacac tctgactgat ttgccccatt
    10741 tcaccgggca attgtggtaa tcgagtggga atattttgtg ccagagtcga gaggccatcg
    10801 gtgatgatat caatattggc gctcaggcga cgggagaaaa ggacaatcaa tagcaggctt
    10861 atcagcaaat cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg
    10921 cgaatatctt cggttaattc attggcccag atatagccga ggatttcacc attacgctca
    10981 atggggagca ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata aaccaaaggg
    11041 gtatttgtac gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gccgacatta
    11101 ttctgatata gcgctgaagg cgcgtaggtg attatcgcat ccagcatttt gttgtaataa
    11161 ccagcaccta tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca
    11221 tttaatgcgc ggatacgctc ctcacgtggt aagtcgatgt agagatcata gcgatcgcct
    11281 agtgcctgat taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact
    11341 gctgaacgtc cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc
    11401 aggattgcca tcaggatcat ttgattgcgt aagcggcgtg gataaatcca cttcatataa
    11461 tgcatgctga aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat
    11521 gatttctcgg cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta
    11581 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
    11641 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
    11701 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
    11761 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
    11821 caacgcacct ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca
    11881 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
    11941 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag
    12001 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctaaac
    12061 cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg
    12121 ccagtggaac gcgataccgc gttgaaagct ttgcatgaac gcatcaataa atatcgaaat
    12181 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
    12241 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
    12301 ggtgtcgagc agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg
    12361 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
    12421 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
    12481 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
    12541 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
    12601 gtgcttgccg gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt
    12661 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
    12721 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
    12781 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
    12841 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
    12901 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
    12961 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
    13021 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
    13081 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
    13141 gtcgatcggc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
    13201 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
    13261 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
    13321 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
    13381 atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc
    13441 ggctgtatag ttttgcatgt ccgcgctgat ggcgattatc tctctatccg tgttcgcgat
    13501 accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
    13561 ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
    13621 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
    13681 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
    13741 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
    13801 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
    13861 gaagatgtgt taattactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg
    13921 accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac
    13981 agtgtggctg ccccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
    14041 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac
    14101 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
    14161 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gtgtcgatgc gcttaatgta
    14221 cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
    14281 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
    14341 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg
    14401 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
    14461 aggaaatcgc gggaatcgta ggacggataa ggtgtttatg ccgcatctgg cattcagtgc
    14521 aaatgccaga tgcgacgctg acgcgtctta tctggcctac tttgatgtaa tctgttgaaa
    14581 taatgggaat cgtaggacgc ataaggcgtt tacgccgcat tcggcaatcg gtgcaaatgc
    14641 cagataagac actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac
    14701 ttaacgtcac tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca
    14761 tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga
    14821 tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga
    14881 gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg
    14941 gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac
    15001 cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt
    15061 tgttgttcat agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga
    15121 ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat
    15181 gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa
    15241 cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt
    15301 catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc
    15361 ttgacataac tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga
    15421 atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat
    15481 acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca
    15541 gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg
    15601 gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc
    15661 agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg
    15721 acgcacaatt gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag
    15781 ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt
    15841 aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata
    15901 ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct
    15961 tcttcctctt caacttccgg gccagcggcg agcattttga tatgcacaga gtagcgtgta
    16021 ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg
    16081 ctcagccaga atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata
    16141 aacgggaagt gcaaattatc catttcatga atacttacgc cttctccggt gtccagaatg
    16201 cggaatgtca ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc
    16261 aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg
    16321 cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct
    16381 ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag
    16441 aacagcaccg tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca
    16501 tcgaccagcc gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg
    16561 agtttagccg cgctctccgc cagggactgt gcaggttctt tcagcgcatc gccaatgttt
    16621 ttcataaagg tcatccggcc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt
    16681 ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc
    16741 gcgacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata
    16801 atcccctgat gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa
    16861 tgatcggcaa ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg
    16921 ccgagcggca gcagtgagac tatcttttca ttgattgccc gtaaaatgcg caattcgtta
    16981 ttgaccgccg tgttgggtag actttctgta ctgcgactgg agaaatggcg gaatgtggta
    17041 tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt
    17101 tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta
    17161 gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc
    17221 gttccttctt tctcattatc attgtctccc gttgccgtcg tgtctggctc aaggcggaaa
    17281 ctgtccagcg gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc
    17341 gttgccagat gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc
    17401 caggccaggc ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc
    17461 aacatttctg cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag
    17521 gtgagatctt tgaggggcag ggttgagatc agtaccagac tgttatcctg accattcagg
    17581 taatacatcg accacggtac attttctgca ccccacaatg tatccagata agtggacatc
    17641 cgctgagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg
    17701 cggcgtggtt tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg
    17761 ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc
    17821 caggtatcga cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct
    17881 ttcagccagg cgttaacggc gctttgtacc attaccccca tcgtcaccag taacacaatg
    17941 atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc
    18001 gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg
    18061 cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct
    18121 cccgtgatat ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta
    18181 caaataacct acaaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat
    18241 tatgctgatg ttaattattt gtgaaatagt tgacaagagt tatagttttt ctgtggtagc
    18301 acagaataat gaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg
    18361 aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat
    18421 agcgggagat attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata
    18481 cttaagaata agttattgat tttaaccttg aattattatt gct
//
LOCUS       DAZPAC010000064        18514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZPAC010000064 DAZPAC010000000
VERSION     DAZPAC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.064"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            76..288
                     /gene="cspH"
                     /locus_tag="ACHUPM_001917"
     CDS             76..288
                     /gene="cspH"
                     /locus_tag="ACHUPM_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR0869705.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            735..1040
                     /locus_tag="ACHUPM_001918"
     CDS             735..1040
                     /locus_tag="ACHUPM_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869706.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1147..1791
                     /gene="gfcB"
                     /locus_tag="ACHUPM_001919"
     CDS             1147..1791
                     /gene="gfcB"
                     /locus_tag="ACHUPM_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR0869707.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1788..2534
                     /locus_tag="ACHUPM_001920"
     CDS             1788..2534
                     /locus_tag="ACHUPM_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0869708.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2534..4630
                     /locus_tag="ACHUPM_001921"
     CDS             2534..4630
                     /locus_tag="ACHUPM_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0869709.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4676..5815
                     /locus_tag="ACHUPM_001922"
     CDS             4676..5815
                     /locus_tag="ACHUPM_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR0869710.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5803..6249
                     /gene="etp"
                     /locus_tag="ACHUPM_001923"
                     /gene_synonym="yccY"
     CDS             5803..6249
                     /gene="etp"
                     /locus_tag="ACHUPM_001923"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR0869711.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6269..8449
                     /gene="etk"
                     /locus_tag="ACHUPM_001924"
     CDS             6269..8449
                     /gene="etk"
                     /locus_tag="ACHUPM_001924"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR0869712.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8564..9862)
                     /gene="appA"
                     /locus_tag="ACHUPM_001925"
     CDS             complement(8564..9862)
                     /gene="appA"
                     /locus_tag="ACHUPM_001925"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR0869713.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="ACHUPM_001926"
     CDS             complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="ACHUPM_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR0869714.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10047..11183)
                     /gene="appB"
                     /locus_tag="ACHUPM_001927"
     CDS             complement(10047..11183)
                     /gene="appB"
                     /locus_tag="ACHUPM_001927"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0869715.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11195..12739)
                     /gene="appC"
                     /locus_tag="ACHUPM_001928"
     CDS             complement(11195..12739)
                     /gene="appC"
                     /locus_tag="ACHUPM_001928"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR0869716.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12873..13730)
                     /locus_tag="ACHUPM_001929"
     CDS             complement(12873..13730)
                     /locus_tag="ACHUPM_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR0869717.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="ACHUPM_001930"
     CDS             complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="ACHUPM_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR0869718.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14122..14709)
                     /gene="hyaD"
                     /locus_tag="ACHUPM_001931"
     CDS             complement(14122..14709)
                     /gene="hyaD"
                     /locus_tag="ACHUPM_001931"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR0869719.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="ACHUPM_001932"
                     /pseudo
     CDS             complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="ACHUPM_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="ACHUPM_001933"
     CDS             complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="ACHUPM_001933"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR0869720.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="ACHUPM_001934"
     CDS             complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="ACHUPM_001934"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR0869721.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4544 a         4913 c         4782 g         4275 t
ORIGIN      
        1 gcggtcttaa atatcgctgg ctttatttta ttaaccccgt atagtgcagc gcagtatttt
       61 cagtaaggaa tttgtttgtc ccgtaaaatg acaggaattg tcaaaacctt tgatcgcaaa
      121 agcggcaaag gattcattat cccctccgac ggtcgtaaag aagttcaggt ccatatttcc
      181 gcattcactc cccgcgacgc agaagtgctt ataccaggat tacgtgtgga attttgccgc
      241 gtaaatggcc tgcgaggacc aacagcggca aatgtttatc tctcttaata tgacgctgct
      301 tgtttaaagc tgactggttt tattggaata gtgcaatagt caacatcata cattataata
      361 tcagttactt ttccacaaag aaactaatcc atttaagcga atatctctgc acctaatagt
      421 tataaatgcc atttaacagc cagttatagt accacttgaa actatattac caacaataat
      481 taatagcttt cgtaacaatt tccttatctt taatttgtat tttgctaaga tttatcttat
      541 gctcacaagt cgtatttcca gaggggatcg ttaataatct tatatttccc tctgcgcacc
      601 tttcctaccc gcagtatcag tatatagtgc aagcggtatg aggaaaggga gatttcacaa
      661 ggatgttata ctcaaagtag acaatcctga tctcaagcgt acgtattgtc catgcgatga
      721 ataaaggaaa agttatgaaa cacaagcttt ctgcaatcct tatggccttc atgttgacga
      781 cgccagctgc gtttgctgcc tctgaagcaa caaacgggac cgaggcaacg acgggtacta
      841 ctggcaccac aacgaccact accggcgcaa ccacgactgc tactaccact ggtggcgtgg
      901 ctgctggcgc tgtcggcacg gcgacagttg tcggtgtggc aacggctgtt ggtgtggcga
      961 cgttggcagt tgttgcagca aatgacagtg gcgatggcgg ttctcataac acctcaacca
     1021 ccacgtccac tacacgctaa tactgcttta acgcgtcgaa attaaagtta aatgataccc
     1081 ggttgcgata ttcgtaccgg gctttagaga tttgcatttt tttaattaac ctttcaagga
     1141 ttaaaggtgc gccctcttat tttatcgatt ttcgcactat ttcttgcggg atgtacgcac
     1201 agccagcaaa gtatggtcga tacatttcgc gccagccttt tcgataatca ggatatcacc
     1261 gtagcggatc agcagatcca ggcgttgcct tattccacga tgtatttacg ccttaatgaa
     1321 gggcaacgaa tctttgtggt actgggatat atagaacaag aacaaagcaa atggttatcc
     1381 caggataacg ccatgctggt tacccacaat ggacgtcttt taaaaaccgt caaacttaat
     1441 aataatctgc tggaagtgac aaattccggg caggaccctc tgcgtaacgc gctggcaata
     1501 aaagatggca gccgctggac gcgcgatatt ctctggagtg aagacaacca ttttcgctct
     1561 gcgaccctga gttctacttt ttcctttgct ggattagaga cgctgaatat tgcgggtcgc
     1621 aatgtgctgt gtaatgtctg gcaggaagag gtgacttcca cgcggccaga aaaacagtgg
     1681 caaaacacat tctgggtcga ttcggctact ggccaggttc gtcaaagtcg gcaaatgtta
     1741 ggcgcagggg ttattcccgt agaaatgacg tttcttaaac ccgcaccatg aataaattac
     1801 agtcgtattt cattgccagc gtactttacg taatgacacc ccatgccttt gcgcaaggaa
     1861 cggtgactat ttatctgcct ggcgaacaac agacactttc cgttggcccc atggaaaatg
     1921 ttgtccagtt ggtgacacaa ccgcaactgc gcgatcgcct ctggtggcct ggtgccttgc
     1981 tgaccgactc tgcggcgaaa gctaaagcgc ttaaagacta ccaacatgtc atggcacagc
     2041 ttgcttcctg ggaagcagaa gctgacgacg atgtggcggc aacgattaaa tccgtgcgcc
     2101 agcagttact caatctcaat atcactggtc gcttaccggt caaactggac cctgattttg
     2161 tacgagtgga tgagaacagc aatcccccgt tggtcgggga ctatacgctc tataccgtac
     2221 agcgtccggt gactattact ctgctgggtg cggtttctgg ggccgggcaa ttgccctggc
     2281 aggcaggccg tagcgttacc gattatctgc aagatcaccc ccgccttgca ggcgcggaca
     2341 agaataacgt tatggtgatt acgcctgagg gtgagactgt cgtcgcccct gttgccctgt
     2401 ggaataaaag acatgtggaa ccaccgcctg gcagccagct ttggttgggc ttttctgcgc
     2461 atgtactgcc cgaaaaatat gctgatttaa atgaccagat tgtgtctgtt ttaacgcaac
     2521 gtgttcctga ctgatgaaga agaattctta tcttttaagc tgcctggcca ttgccgtctc
     2581 cagtgcctgt catgctgaag tattaaccta cccggatccg ctgggttcgt cgcaatcaga
     2641 ctttggcggc acaggattgt tgcagatgcc aaatgcgcgc atcgcaccgg aaggtgaatt
     2701 cagcgtcaac taccgggata acgatcaata ccggttctac tccacctccg tggcgctgtt
     2761 cccatggctg gaaggcacca ttcgttatac ggatgtgcgc acacgcaaat atagccagtg
     2821 ggaagatttc agcggcgatc agtcatacaa agacaaatca ttcgatttta aacttcgcct
     2881 gtgggaagaa ggttactggc taccgcaagt ggcgtttggt aaacgtgata ttgctggtac
     2941 gggtctgttt gacggtgagt atctggtggc cagcaagcaa gcggggccat ttgatttcac
     3001 cctcgggatg gcatggggct acgccggtaa tgcgggcaat attaccaacc cgttttgccg
     3061 ggtgagcgat aaatattgtc atcgcgcaga gtctcacgat gcgggcgata tcagctttag
     3121 cgatatcttt cgtggcccgg cttccatctt tggcggcatt gagtatcaaa cgccgtggaa
     3181 tcccctgcgt ctgaaactcg aatacgatgg caacaattac cagaatgatt tcgctggcaa
     3241 actgcctcag gcaagccatt tcaacgtcgg cgcagtttat cgcgctgcca gctgggcaga
     3301 tctcaacctg agttatgaac gcggtaacac gttgatgttt ggcttcacgt tacggaccaa
     3361 tttcaacgat ctgcgccctg ccctgcgcga tacgccaaaa ccggcatatc aacctgcgcc
     3421 tgaatctgaa ggattgcagt acaccacagt agcgaaccaa cttaccgcgc tgaagtacaa
     3481 cgcaggtttt gaagcaccgg aaattcagct gcgcgataag acgctgtata tgtctggtca
     3541 acaatacaaa taccgtgatt ctcgcgaagc ggtcgatcgt gccaaccgga ttctggtgaa
     3601 taacctgccg caaggcgttg agaagattag cgtgacgcaa aagcgcgagc atatggcgat
     3661 ggtgactacc gaaaccgacg tagccagcct gcgcaaacag ctggctggta cagcgcctgg
     3721 tcaatcagag caacttcaac aacaacgtgt tgaagcagaa gatctttctg cctttggtcg
     3781 gggctaccgt attcgtgaag atcgctttag ctactctttc aacccaacac tttcacagtc
     3841 gctgggcggc ccggaagatt tctatatgtt ccagctgggg ctgatgtcca gtgcccgcta
     3901 ctggtttacc gaccacctgc tgcttgatgg cggtattttc accaatattt acaacaacta
     3961 cgacaagttt aagtcttcgc tgttgcccgc ggactctacc ctgccccgcg tgcgcacgca
     4021 tatccgtgat tacgttcgca atgacgttta tctcaacaac ttgcaggcga actactttgc
     4081 cgacttaggc aatggtttct atggccaggt gtatggcggt tatctggaaa cgatgtacgc
     4141 cggtgtcggt tccgagctgc tttatcgccc gctagatgcc agctgggcgc tgggtgtgga
     4201 cgttaactac gtgaagcaac gtgactggga caacatgatg cgcttcaccg attattccac
     4261 gccaactggt ttcgtgacgg cttactggaa cccgccgacg ctcaatggcg tactgatgaa
     4321 acttagcgtt gggcaatatc tggcaaaaga taaaggggca acgatcgacg tcgccaaacg
     4381 ctttgacagc ggcgtggcgg taggggtatg ggcggcaatc agtaacgtat ctaaagatga
     4441 ctacggcgaa ggcggcttta gtaaaggttt ttatatctcg attccgttcg acttgatgac
     4501 cattggacct aaccgcaacc gcgcggtggt ttcgtggaca ccattgacgc gtgatggtgg
     4561 acaaatgctg tcacgcaaat accagctcta tccaatgacg gcagagcgag aagtaccggt
     4621 tggacaataa cctgacgtaa ttcataacat tacattgact gataaatata aaccaatgaa
     4681 aaagaacatt tttaagtttt cggtactgac attggctgtt ctgtctttga cagcatgtac
     4741 ccttgttcct ggtcagaatt tatcgaccag taacaaggat gtcattgaac tacctgacaa
     4801 tcagtatgac ctggacaaaa tggtcaatat ctatcccgtc acgccaggat tgattgatca
     4861 gttgcgcgcc aaaccgatta tgtcgcaggc taacccggag ctggagcagc aaatcgcaaa
     4921 ctatgaatat cgtatcggga ttggtgatgt gttgatggtt acggtctggg atcacccgga
     4981 actaaccacc cccgcagggc aataccgtag cgccagtgat accggcaact gggttaacgc
     5041 cgatggtgcg attttctacc cctatattgg tcgcctgaaa gtggccggga aaacacttac
     5101 ccaggtacgc aacgagatta cagcgcgtct cgattcagtc attgaaagcc cgcaagtcga
     5161 tgtcagcgtc gcggcgttcc gatcgcaaaa agcgtacgtc accggcgaag tcagcaaatc
     5221 gggtcagcag ccgattacca atattcctct gaccatcatg gatgcgatta acgccgcggg
     5281 cggtctgacc gcagatgcag actggcgcaa cgtggtgctc acccagaatg gcgtaaagac
     5341 caaagtgaac ctctatgcgc tgatgcagcg tggcgacttg cgccagaaca aactgctgca
     5401 cccgggcgat attttattta ttccgcgcaa cgatgatctg aaagtcttcg tgatgggtga
     5461 agtgggtaaa cagagcacgc tgaaaatgga ccgcagcggt atgaccctgg cggaagcgct
     5521 gggtaacgct gagggtatga atcaggatgt cgctgatgcg acggggattt ttgttattcg
     5581 tgcaacgcaa aacaagcaga atggcaaaat cgccaatatt tatcagctga atgcgaagga
     5641 cgcatcggca atgatactcg gaacggaatt ccagcttgaa ccttacgaca ttgtttatgt
     5701 cactaccgcg ccgctggctc gttggaaccg tgtgatttct ctgctggtac cgacgatttc
     5761 cggggtgcat gacctgacag agacttcacg gtggattcaa acatggccca actaaaattt
     5821 aactcaatcc tggtggtttg taccggcaac atttgccgtt cgcccattgg cgagcgcttg
     5881 ctgcgtaaac gtctgccggg cgtaaaagtc aaatcggcag gtgttcatgg tctgataaaa
     5941 caccctgcag atgcgacagc ggcagatgtt gccgctaatc atggcgtatc tcttgaagga
     6001 catgccggac gtaagctcac tgctgagatg gcacgaaatt acgatctgat tctggcaatg
     6061 gagtcggaac atattgctca ggttaccgca attgctcccg aggtccgcgg gaagacaatg
     6121 ctgtttgggc aatggctgga acagaaagag atcccggatc cctatcgtaa aagtcaggac
     6181 gcatttgaac atgtctacgg tatgttggag cgcgccagtc aggaatgggc gaagcgcctc
     6241 agccgataat ttgagtttat aaaatacgat gacaactaaa aatatgaata cgccaccagg
     6301 cagcactcag gaaaatgaga tcgatctgct tcgtctggtc ggcgagttat gggatcaccg
     6361 taagtttatt atcagcgtga ccgcgttatt cacgctgatc gctgtcgctt actcgctgtt
     6421 aagcacacca atttatcagg cagatactct ggtccaggtt gagcaaaaac agggcaacgc
     6481 cattctcagc ggcctgagcg atatgatccc taactcatcg cccgagtctg caccggagat
     6541 ccaactgctg caatcgcgca tgattctcgg taaaaccatt gctgaactga atctgcgcga
     6601 catagttgag cagaagtatt ttccgattgt gggtcgcggc tgggcgagat taaccaaaga
     6661 aaaaccaggt gagctggcga tcagctggat gcatattcca caactgaatg gtcaggatca
     6721 gcaactgaca ctcacggttg gggaaaacgg ccactataca ctggaaggtg aagagttcac
     6781 cgtcaatggt atggtcggcc agcgtctgga aaaagatggc gttgcgctga ctatcgcgga
     6841 cattaaggcc aaaccaggaa cacagtttgt cctgagccag cgtaccgaac tggaagcgat
     6901 taacgcattg caggaaacct ttaccgttag cgaacgcagt aaagaaagcg ggatgctgga
     6961 acttaccatg actggtgatg atccccagtt gattactcgt attctgaaca gcatcgctaa
     7021 caactatttg caacagaata tcgctcgcca ggcggcgcag gattcacaaa gccttgaatt
     7081 cttacagcgc cagttacctg aagtgcgcag cgagctggac caggcggaag aaaaactcaa
     7141 cgtttatcgc cagcagcgcg attcggttga ccttaacctg gaagccaaag ccgttcttga
     7201 gcagattgtg aacgttgata atcaactcaa tgagctgacc ttccgcgagg cagagatctc
     7261 ccagctgtat aagaaagatc acccaactta tcgtgcgctg ctggaaaaac gccagacgct
     7321 ggagcaagaa cgcaaacgcc tgaataagcg ggtatcggca atgccttcca cccaacagga
     7381 agtgttgcgt ttaagtcgtg acgtagaagc gggccgtgcg gtatatctgc aattacttaa
     7441 ccgccagcag gagttgagta tttcgaaatc cagtgccatt ggtaacgtgc ggattatcga
     7501 cccggcagtc actcagccgc agccagtgaa accgaaaaaa gcgttgaatg tggtgcttgg
     7561 ttttattctt ggcctgttta tttctgtggg tgccgtgctg gcgcgtgcga tgttgcgtcg
     7621 tggtgtagaa gccccggaac aactggaaga gcacggcatc agcgtttatg ccactatccc
     7681 aatgtccgag tggctggata aacgcacccg tctgcgtaag aaaaatttat tttctaatca
     7741 gcagcgccat cgtactaaaa atatcccctt cctggcggtg gataacccgg cggattctgc
     7801 tgtggaagcc gtacgtgcgc tacgaaccag tctgcatttc gctatgatgg agacggagaa
     7861 taacattctg atgatcaccg gtgcgacgcc agacagtggt aaaacgtttg tcagttcaac
     7921 tctggcagcg gtgatcgccc agtccgatca aaaagtgtta tttattgatg ccgacttacg
     7981 ccgtggttat tcgcataacc tgtttaccgt gagtaatgaa catggcttgt cggaatatct
     8041 ggcaggtaaa gatgagctca acaaagtgat ccagcatttt ggcaaaggag gctttgatgt
     8101 gattactcgc ggtcaggtgc cacctaaccc gtctgaactg ctgatgcgcg atcggatgcg
     8161 tcaattactg gaatgggcga acgaccatta cgatctggtg attgtcgata cgccgccgat
     8221 gctggcggtg agtgatgccg cggtcgtggg gcgttctgtt ggcaccagcc tgctggttgc
     8281 gcgttttggc ttgaacaccg ccaaagaggt gagtttgtca atgcagcgtc tggaacaggc
     8341 aggcgtcaat attaaaggcg ctatcctcaa tggtgtgatt aaacgcgcca gcaccgctta
     8401 cagttacggc tataactatt acggttatag ttactccgag aaagagtaaa actgaaagtg
     8461 gccggatgcg gcgtgaacgc cttttccggt ctacgcatta gacagttctt cgtttgtcat
     8521 cagcctcaga gcattcaggt aactgaatgc tcttttttat gcattacaaa ctgcacgccg
     8581 gtatgcgtgc ttcattcacg atttgcgtaa aacctgccaa cgaacacatg ccctgcgcat
     8641 ttcgctcttc acatcctgcc agggtcagtt tcacctctcc gggcggcgta tttaatgaca
     8701 gcggcgtttt atcacgcatc tgctgtaaag tctggaagac cagcgaaacc tgaatccact
     8761 ggctgttatc gcttagccga cgccagcgtt caaacaccag ttcaccacct ggcggcgtgt
     8821 tatccggctg accgggaagc gtccagttga gctccagtgc gccgccgaga tttgccagat
     8881 tagtatcgtg tccggcgata aacagcactg aagtgggtaa tgtcacacca tacgcctgtt
     8941 tttgcggtgg atggggcgtc aacgctgtct tgatcaaatc taataacggg gtggcgcggc
     9001 tgcgggcaac ctctggcgtg cgttgtagca aataaaattg cgcgttatgc aaacttagca
     9061 aggtgttcca ctggtgtgaa tcggtgatcc ttccccaccc cggctccggc attccctgtg
     9121 cttgttgcag gagaaatatc tccgtcagca ttgatgcgag gcttaccgca ccggttaatg
     9181 agacattgtc ggcgctcacc ttgagttccg atggtaatgc ctgcgttaat gaacagcttt
     9241 cgtcctgttt ctcacgttta aggcacaagt ttgattgcgg aaaattaagc acccgttcca
     9301 gttcgcgaaa cgccgtttgc cgatgcccgg taaagtcagc aattgaccct cctgccctgc
     9361 tgaggatcgc gtcagtcacg ttcgcgttat ccagttggca aacgccagtt tttagaggat
     9421 taaataacgg atcgggactg gacgtatcag cctgggtatg tacggttatt gcacagtcag
     9481 gtgccagccc ggcggcgaag gcttcgcctg ttttacgggt acgctcgtcg acatcagcaa
     9541 taatcgcgac ctgaccagac tgcgggcagc cctttttcgc cagcaatccg tcggctacca
     9601 gacgctggcg ttggtaatgt ccgagatagg cgattagctc accaccgcgc ggtgtcagcc
     9661 aacccagttt taccggccag gttggccatg cgtctggggt gacatcctgc atcagttgcg
     9721 tggccttggt tggagcacgc acaccatgac gactgacaat caccacactt tccagcttca
     9781 gctccggctc actctgagcg aatgcagatt gcggggttaa cggaatcaga agagataaaa
     9841 atgggattaa gatcgctttc atcgatatgt ttccgctttt catatctgac attattgatt
     9901 gcctgatgcg atgctaatgc gccgcatcag gcctacgttc gttaactttt cagacgcggg
     9961 tccagccata ccaacacaag ggtggagagc gaacacatca acaaaatgcc gacgaaccaa
    10021 agtaaatacc acattgttcc tgctccttag tacaactcgt tttcgttacg gcggagagtt
    10081 tctgttgtca tgcgtcccca cattttgtag tagctccaga gagtgtagag caacacaatg
    10141 ggcaaaaata tcagcacgat taccaacata atgctcagcg tcagctgact ggaagtgctg
    10201 tcccacaacg tcaggctgga gattggactc acgcttgacg gcatgacaaa ggggaacagc
    10261 gtgatgcctg ccgtgaaaat cacgccaaat tgcatcaatg acgccattaa aaatccccaa
    10321 cccgggcgac cacgataaat cgccatcacc gtcagcaatg ggcagaagaa ccccagcagc
    10381 gggaagatcc acaaaacggg cgattcgacg aaattattca tccaggcacc aggtagcact
    10441 gccaccagtt tcattaacgg attggaagga ccgttagcat cctgggcgag cagtacaaag
    10501 ccatcaatac cgacccacag ccagtaaccc gccagcaaaa agcacagcat caccaacagt
    10561 gcagcgcgtt tggtggccag ctgtgaacgc agatgaatca caccaacagt tttcagttgt
    10621 aaccagacgc caccttgcaa aatcaccatc ccaaggctga gcaatccgca caataaaggg
    10681 aatggcgtca gcagttgcca gaagctgccg agatactcca cgcgtaattg cggtgtgaag
    10741 gcaaacggca cgccgagcaa caagttgccg aaggcgatac cgaagactac cggcggcacc
    10801 agactgccga tgaccagacc ggcgtcccac attttacgcc agcgtgcatc ggcgattttt
    10861 ccgcgataat caaaggccag cgggcggaag aacagtgagc acagcaccag gatcatcgcc
    10921 acataaaagc cggaaaacgc cgctgcatac actctgggcc aggcggcaaa taatgcccca
    10981 ccagcgagga tcaaccagac ctggttgcct tcccagtgtg caccaacgct gtttatcact
    11041 atccggcgtt catcatcatt acgcgccacc agcggcagca gacagccgat ccccatgtca
    11101 aatccgtcgg agatcataaa gaccaccagg atcacgccaa tcagcagcca ccagatgaag
    11161 cgcaatgttt cataatcaaa catgattttc ccctttaccc ctgttgctgc gtcggttgtt
    11221 cactctgcat cgcgctcggc cccagacggg catatttctg catcaggtag acttcggcga
    11281 ttaagaacag ggtgtaaagc cctacgatca tgatcagtga gaaagccagt tgtcctgtgg
    11341 ttaaagcgga gtgcgcggag tatgtcggta agatgtcctg tatcgcccac ggctgacgac
    11401 caaactcggt cataaaccac ccggcttcaa tcgcaatcca cggcaacggc aaactccaga
    11461 gcgccatttt cagcacccag cgatgctggt cgattttgcc acgcagcgtc tggacaagcg
    11521 caatcagcat caccagtagc ggcagggaac cacagcccac catgatgcgg aaactccaga
    11581 ataccggcgc aacctgaggt atcgcgccac gcatcgccgc ctggtactgt gcggctgtga
    11641 catgattcat atccggcgca tagcgggaga gcaacatgcc gtagcccagg tcaccttcca
    11701 gttcccggaa tgcctgcaac acatgcggct cacgattgcc ttgcgatatt tcctgcatta
    11761 acagccaggc catacgtccg cgttgcaagc gtgggtaggt ttcagccatc aaatttttca
    11821 gacccggcac ggttttatct aatgagtgag tggcgaggat ccctaacagc gcgggaattt
    11881 tgatggcaaa ggcgttacgc tcttgatcct gttccggcca ggcaaccaca tggaacggtg
    11941 caggagcagg ttccgtttgc cactcccctt ccatcgccgc cagttttacc ggttgtactt
    12001 gcgcgacttc atacgcagaa ctgtctccga gttgtagcgt gccgataatc gccagagtac
    12061 cgaagacgga accgatggca aacgagcgta atgcaacatc gcgctcccgt ccgcgcagta
    12121 aataccaggc gctgatcgcc ataataaaca tggccccggt cacgtagccc gccattacgg
    12181 tgtgcacaaa tttcacctgg ctgaccgggt taaagaccag ctcgctgaag ctggtcatct
    12241 ccatacgtag ggtgtcgata tcaaaatgcg caccggtcgg gtattgcatc caaccgttgg
    12301 cattcaatat ccacaatgca gagagatttg aaccgaacgc caccagccac gtcaccagca
    12361 ggtgctggta tttattcagc cgctgccagc cgaagaagaa aagcccgaca aaggtggatt
    12421 cgaggaagaa ggccattaat gcttccatcg ccagcggtgc gccaaaaata tcgcccacat
    12481 agttggaata gaatgaccag ttagtaccaa actgaaactc catggtcagg ccggtagcca
    12541 cgccaagagc aaagttgata ccgaagagct taccccagaa gcgcgtcata tcgcggtaga
    12601 ttgttttgcc ggtgaccacg taaatggttt ccatgatagc cagcaaaaaa atcagcccca
    12661 gggtaagggg tacaaataaa aagtgataca gcgcggtcag agcaaactgc cagcgcgata
    12721 aatcaatgac atcccacatg cgcactcctg taggcgaaga gagaaaaata acccgataat
    12781 gttagatctc cctctttatc ctgaaactct acgaccagag gtattcgccg attgcgatat
    12841 tcataagact gaaagcgata cttaccgtct ttttacgtcg gtgcagcttc cgccagccac
    12901 tgacatacct cgctaagccg ctgcgcagag tcgaccaaat cttctggcgc tgccagcacc
    12961 acttccggta ttgggcagat ttcataactt tccagtaaag gaccttttaa ggtgtccgta
    13021 cagcgtaaat gccagacatg gcgtaacccc gtggcgttga tatagctctc gccatagcca
    13081 atggtacgaa tctgaatatt tcccggccca cagagacgtg agagaaaaag ccgatcagcc
    13141 tcgctgatgg gtaattgcgt cagattaatg ctgtgcggct gcgcgtcggg gttacgtaca
    13201 tgcgccagta actcatgcgc caacggtagg ccattcatca ggccatcaat gggcggcggt
    13261 aacagcgaat ctgtcggcaa gagattttgc gtggccgcct gccacaacgc cagcggcgcg
    13321 cagccggcct ccagtttgtc ctccagcaac ttttcgccgc gacgtttgcg cacccgccat
    13381 aatccgcaga agatcgcctc ctgtatttca ctttcgctgt cgtcagcctg ctgaatgcgc
    13441 actgacactt ccccttcccc gagaagcgta ttgataaagt gacgatcgtc ggcgttgaga
    13501 tttaacagat cgactgccag cacctcgccc aacggcggct ggcgttgact tagttgatgt
    13561 tgtaactcgt ttaacagcgc aatcacctgc gggctgtgag cacattgctc cagggccgcc
    13621 atactcggtt catcattcac ctgacagctg atcggcagtg gattcatgct gaaactgtca
    13681 tcgttcggtt gcgttcctgg ccccagcaga tggaaaaaag tttcgctcat gaggcacgct
    13741 cctgttgtgg ttcgacaagc ccacgcatca ggtttatcag ttccgctcac gggtgaatac
    13801 cattcagcac accgcgatag tttccgccgg taaacactaa tgtggcggga aagcgaaaga
    13861 cgccaaagcg atctccgatg gcttcgctct gttcaaggtc agcaatcgcc acctgccatg
    13921 tgtagccggg aaactcgcgc agtaattcgc caatcattac cggattatcg ctgacctctg
    13981 gcgtgcgttt cgggtcactg cttaataaca ccacgccgtc tggcgcttgc gtaagccagt
    14041 cgtcaagacg gaattcacta accggcgtcc agccgcgcgc cagcattcgt tgccacaacg
    14101 catcaaatgg cgtgtcgttg ctcatccctg ctcctccagt gtcatcctgt actggcgcaa
    14161 gcgaacgcct tcgtaatttt ccatcgacag acagtcataa ttgagacagc gcgattcatt
    14221 agccggttgc ggcacaattc cccatgcagc cagctgcgcc agcgccgcct gttccgctgc
    14281 gggcagttgc tcccgtgcca gttcgctcag gctgccgccg tagtcgtcga gcattgcggg
    14341 ttgcagaccg acgagggcaa tatgtgctgg cagatgtccg cggatatctg ccagcgccag
    14401 cacttcggag aaactgttct ggtgcaggct cattttcttc gcgctgagat aagccggaat
    14461 acgttctccg gcataggttc gcagcgttcc aggttccagc ccgtagtcaa tggcatcgag
    14521 aatcaacaga tggctggcgc tttcgacata ccccagcaag ttcagtccct gagtaccgcc
    14581 atcgacaatc tccacatact cgggccagtg gtaatgggca tacaaccgtt ccgccacccg
    14641 cacgccgaaa ccttcatcgg cccacagcag gttgcccagc cccattacca caacgcgttg
    14701 ctcgctcatg aacgctcctt gttacttatt ttgccaaatt tgtggctacg gtagccgttg
    14761 accatggtgg agatcaccgt gtcgtcggac atgatgtctt cacgcagcgc catatagaca
    14821 tgaccgatca caaacgcgcc aatcagccac atccccagcc gatgccagct gtgaatgtcc
    14881 attgagttgc ctcccgtcca gtagaaaaat tccaccacat aacggaacgg cgcaaaaata
    14941 gcgtactggc tgtgttcgct gtacagcgca aagccggtga tgatcataaa gaccgacatc
    15001 aggaaatagc cgaacatcgc cgcctgggcg atggggttat ggcctatatc ggcactcgga
    15061 cgttttgcca gaacagatac cagcggattt cataccacac gccctgccac cagcttttac
    15121 gccataccgg cacgataaac agctcgcggg agtatcgatt gccaacaaaa gcccagtaga
    15181 tccgcatcag caagaccacg gtaaaaacca tcccggcgct gaagtgaatt aacctgatgt
    15241 agcccatata gaacagatac gtcgcctcgc cgctgacgga aggtagcggc ttgccgataa
    15301 agtatccggt gaccatcaac accgccatgc ataacaccgt cagccagtgc cagatgcgga
    15361 ctggcgcttc aaagacatag tggctgacaa cgttgtcgct tttctgttgc atgatgattc
    15421 tccttcgctg ttaacgcacc tgcacggaga tcagctcgct accgtcgtcg cccagcacgt
    15481 gtgttgaaca ggcgaggcac gggtcaaagc tgtgcagagt acgcaggatc tccagcggtt
    15541 gctcggggat cgccattttg gtgttcatca gcgccgcttc ataagcgcca atctgccctt
    15601 tgggatcgcg cgggctggtg ttccaggtgg tcggcaccac gcactggtag agatcaatct
    15661 tgccatcgcg aatggcggcc cagtggccta acgccccgcg cggcgcttcg gtaaagccga
    15721 cgccacggca ctctgtcggc caggttgcag gttcccattt ttccgtggag gcggtggcaa
    15781 gattgccgtt tttcaggtta gtcatcagct tgttgaagaa atattgcaac ttacctgcgg
    15841 cccactgtgc ttcgtgcgcg cggcacaaaa tgcggcccaa cgttgactgg ataccggaaa
    15901 gcggcaggtt cagcgccgac atcatgcgat cgaccgactc aacggtcgca gcatcgcctt
    15961 tgtgataagc gattaacgtg cgtgccagcg gccccacttc catcgcgtta ccgcgccagc
    16021 gcggcgcttt gatccacgag tagcgttcct gttcattcag ctgctgaatg ttggtatcgc
    16081 tgcctttgac atcgccgggg ttgtaccacg ggtcggtaat gccatcgaat ggatgacgcc
    16141 cgacctgatc gttgggataa cgataccagg cgtggtcgac aaactcctgc acctgctgcg
    16201 gatcaaccaa atccactggc agcacattgt tgaagtcgcc gttaatcacc gcgccgccag
    16261 gcatcagcag acttttctca ccaaagtcgt tggcaatatc cgggaacgca ccgtagctga
    16321 ggacacattt atcagaaaga cccgtgccga tttcgctcca cggtttgttg aactgaccga
    16381 tggctaaggc gtcggggatc atcacgttgt taatgaagtc cgccgtgcgg gtgataatcg
    16441 actgcaccag gttcaggcgt tccatattga ccgccccgac cgcgccgctt tcgtcaatgt
    16501 taatggcgca aggcatcccg ccgacaatcc agtttggatg cgggttttta ccgccaaaga
    16561 ccgcgtggat tttgacaatt tcacgctgga aatcgagagc ttcgagatag tgggcaaagc
    16621 ccatcaggtt agcttctggc ggcagtttgt actgcgggtg cccccagtag ccattgcgga
    16681 agatccccaa ctgcccgcct tcaacaaatt ttttcaggcg gttttgtacg tcgaagaaat
    16741 agccagggga tgatttcggc caagaggaga gactttgcgc cagttcggag gttttccgcg
    16801 ggtcggcttt cagcgcatct aacacatcga tccagtccat cccggcaagc tgatagaagt
    16861 gcaccagatg atcgtggcac cagagcgttg ccagcataat gttgcggatg atattggcgt
    16921 tgtccggcac tttaataccg atagcatctt cgatggcgta aaccgaagcc agggcgtgta
    16981 cgccagtaca gacgccgcag atacgttcaa cgaacgccca cgcatcgcgc gggtcgcgcc
    17041 cttgtaggat gatctccagc ccgcgaaaca tggtgccgca ggagacggca ttggtgatca
    17101 cattctgatc gttaatattc acttcgcagc gcatgtggcc ttcgatgcgc gtaatcgggt
    17161 cgaccaccag gcggcgtccg gcattattga tggtgtatcc ctgagtttcg tactgagtgc
    17221 tcatgcctgt ttatcctcat tgcctggctg atgttcggtt tctgtaggtt gctggttatg
    17281 acgtctgcgc tggtcaacgg cgctggcgac tgcgtgcaca ccaacagccg ctgccaccac
    17341 accaagcgcg gttaaaccga cggtatcggc ggtggaatga gtacccattt gcggaatatc
    17401 gaccacgcgg ctgtagaacg aaccgcaatc ccagaaacca ttttccgcac agcccaggca
    17461 gccgtgacca gactggattg ggaaagaaac gccatcattc cagcgtgtgg aggaacaggc
    17521 gttataggtg gtaggccctt tgcagcccat tttgtacagg cagtaacctt tgcgggcagc
    17581 gtcatcatcc caactctgga cgaactctcc ggcgtcgaag tgggcgcggc gatagcattt
    17641 atcgtggatt cgctgaccat agaacatcag cggacggccc attctgtcga catctggcaa
    17701 gcgatcaaag gtcaccatgt aagtaatgat ggcgctcatc acatccggga tcggcgggca
    17761 gccaggtact ttgataatgg gtttgtcggt gatgactttg tcgataggcg ttgcctgcgt
    17821 cggattgggc cacgcggcct gcacgcagcc ccaggacgcg caggttcccc aggcgataat
    17881 cgcgctggct ccggcagcgg cacgtttgag tttctcaata aacggtcgac cgctgctgat
    17941 acagaacatc ccctgctcgc ccagcggcgg attaccttct actgcgagga tatatttgcc
    18001 attgtattgc gtgatgatgt cttcgaagac ttcttccgcc tgggttccgg cggcagccat
    18061 caaagtatcg tcataatcga gggaaatcag ggaaaggatg acgtccttcg ccagtgggtg
    18121 agcggagcgg ataaaagatt cggtacagca ggtgcattcc agaccgtgga tccataccac
    18181 cggaatgcgg ggtttgttct ccagcgccca ggcaatcttt ggtgccattc ccgcgcctaa
    18241 tcccagcgac gtggcagcca gactacaata tttgagaaag ctgcgccggg taacgccctg
    18301 acgccgcatg gcctggtaaa atgtttcctc gttattcata tcgcacgtct cttctccttg
    18361 cgacaccggc aggacaccgg gcgcttatgg tcggcaagcg acgtccctgt agcaaggcaa
    18421 accatcgctt cacgtgagat gctgaaaacg aaagctcatc cttctgcact ggcgcacgtc
    18481 gccagaaagt attgttaata aagcgtagtg aaac
//
LOCUS       DAZPAC010000065        18238 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZPAC010000065 DAZPAC010000000
VERSION     DAZPAC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18238
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.065"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            22..3789
                     /locus_tag="ACHUPM_001935"
     CDS             22..3789
                     /locus_tag="ACHUPM_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFR0869722.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADNSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYINKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILIDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGSVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEMR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAAAPASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVNHVTEDIPLQPEPF
                     LPESDGPEEYEDYSAYQELMNNENPEHLQENSDMPSQPDTGHVQADESELQATTATIE
                     PAVRDEPTPQQPVQSTSPSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSAETTTTP
                     PIPDSIVYAPERSDAPISLNMDEILKELDWEKRADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATKFYGGVIELTGSDAFKQKAMQLIIEHNIDVRMKLPDQRAE
                     LEKLRKEMAVSKDAVVTHQPTPELNRNIPEQPAVPDPVQEKEATQSPASPAAPEASTV
                     STAPASSPAEPGKTADAAPGEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEIKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            3807..4154
                     /locus_tag="ACHUPM_001936"
     CDS             3807..4154
                     /locus_tag="ACHUPM_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR0869723.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            4151..4843
                     /locus_tag="ACHUPM_001937"
     CDS             4151..4843
                     /locus_tag="ACHUPM_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFR0869724.1"
                     /translation="MTENTELSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTSQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFVGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            4854..5837
                     /gene="traN"
                     /locus_tag="ACHUPM_001938"
     CDS             4854..5837
                     /gene="traN"
                     /locus_tag="ACHUPM_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HFR0869725.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAMLRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            5840..7129
                     /gene="traO"
                     /locus_tag="ACHUPM_001939"
     CDS             5840..7129
                     /gene="traO"
                     /locus_tag="ACHUPM_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFR0869726.1"
                     /translation="MSAEQDAGKSGKKLAALLGLGSIILFGGGYIAFSKLSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDSQ
                     EKQQQPAAKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATA
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNQSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQTEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            7129..7833
                     /gene="traP"
                     /locus_tag="ACHUPM_001940"
     CDS             7129..7833
                     /gene="traP"
                     /locus_tag="ACHUPM_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HFR0869727.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSHLNGMMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMVWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            7833..8360
                     /gene="traQ"
                     /locus_tag="ACHUPM_001941"
     CDS             7833..8360
                     /gene="traQ"
                     /locus_tag="ACHUPM_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFR0869728.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            8411..8815
                     /gene="traR"
                     /locus_tag="ACHUPM_001942"
     CDS             8411..8815
                     /gene="traR"
                     /locus_tag="ACHUPM_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HFR0869729.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKYLLLIPMMLLPKA
                     VLADGDLADMVRNVGEGAKTAQSSSITIAQFIGVILFLGGLIGLKKVGKQGGMGLAGC
                     IVSIVIGAVLVAGPEMMSRSQKQLGISSISIG"
     gene            8879..9067
                     /gene="traS"
                     /locus_tag="ACHUPM_001943"
     CDS             8879..9067
                     /gene="traS"
                     /locus_tag="ACHUPM_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HFR0869730.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            9051..9851
                     /gene="traT"
                     /locus_tag="ACHUPM_001944"
     CDS             9051..9851
                     /gene="traT"
                     /locus_tag="ACHUPM_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HFR0869731.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            9941..12985
                     /gene="traU"
                     /locus_tag="ACHUPM_001945"
     CDS             9941..12985
                     /gene="traU"
                     /locus_tag="ACHUPM_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HFR0869732.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGLGSLESLITSMSDSLNTAY
                     KNSGHKISCVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMTLAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAFEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEDLVDSALQDSGLYEKHDAAWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEDLASELIRKQGYNL"
     gene            12985..13599
                     /gene="traV"
                     /locus_tag="ACHUPM_001946"
     CDS             12985..13599
                     /gene="traV"
                     /locus_tag="ACHUPM_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HFR0869733.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRLFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRYQCFPLAPLPEDRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            13566..14768
                     /gene="traW"
                     /locus_tag="ACHUPM_001947"
     CDS             13566..14768
                     /gene="traW"
                     /locus_tag="ACHUPM_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HFR0869734.1"
                     /translation="MQRKTLLAALIATLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            14797..15381
                     /gene="traX"
                     /locus_tag="ACHUPM_001948"
     CDS             14797..15381
                     /gene="traX"
                     /locus_tag="ACHUPM_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HFR0869735.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            15478..17646
                     /locus_tag="ACHUPM_001949"
     CDS             15478..17646
                     /locus_tag="ACHUPM_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFR0869736.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTVKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQRAADEYERAVQKSLPIDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMGAYK
                     TQLQNSTYTPTIGTITTQDESNAANSTDPQSVVMKSIAPTILKWTNQAATEWSGTGTT
                     SNQVNPLIKMKNIGDYTLGTTEILWTGYTTVRVLATMGDNSIFGKAVNLFSGLPKGFV
                     ALLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWA
                     ATHLGTSEDRGSRAAYGYIYLIDSMIRPPLMVFGFFFASVAIIAVGTILNSLFGAALV
                     NVQVKSLTGIFSLAGFLLIYARMCTTSVAAIFALQAYLPDHVINFLGGRDGVNTLGNL
                     TSSVKDIFAGSNRNIRHSPGVREDRLKNVTKDDNKDGIKG"
     gene            17720..>18238
                     /gene="excA"
                     /locus_tag="ACHUPM_001950"
     CDS             17720..>18238
                     /gene="excA"
                     /locus_tag="ACHUPM_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFR0869737.1"
                     /translation="MQMVKTINRYPAWWTLYYVLRFIYFISIPVLSIFILFGVLSITS
                     SRYVTQEDYIYTCVCLFLLIAPAILMYSRASSRKDKIKKIVAEIKNTGFYSPDKEYEG
                     LSFTQGVYFGVDTKKGTMLYARAYPGNIMDIIGFDIDNFTRTVTDAKTLEIYTKYINI
                     PMVSIPSGCIHPK"
BASE COUNT         4953 a         4724 c         4512 g         4049 t
ORIGIN      
        1 cttttttcag gagaagcact catgccgtct gtatcccgct accgcacctg gcttgccgtg
       61 cctgcagatg aaatagaaga tctcaaaaaa gcacatcccc cgatgaacgg gcacacgccc
      121 gtcatctggg acaaggagca caaactctgg ttcgcccgcc cgggggcgga cctgtccagg
      181 ctggaccgct ggctgccccg gccacaggac gtctccatga acggcagtga tccggtcacc
      241 gagtttgccc aggttctgga gaatgccgga ctggttctga aagaactgcc ggtcatggac
      301 gggaaaatcc accgtgtccc gaccgccgat gacaaaaaag gacaaaaaag tggcgcatat
      361 cgcggattcc tggacggcag acctgccggc tggtacaggg attatcgcag cgcggataat
      421 tcgccgatca cctggacctt ctccggcgga gaacagacag atccccgcgc aagactccac
      481 ctgaaagcgc attcaatgca acgcagggag gacgcggagc gggaactgaa agcacagtac
      541 aaccgacagg ccgcgtatgc ccgccgctat atcaacaaat ggccgcaggc cacagcacat
      601 gaatacctga cccggaaagg cattcaggct gcaccggggg tgcgggtaaa caacaaaaat
      661 gaactggtta ttcccttcag taaccgtaac ggcgcgatac gctcttacca gcgtattccg
      721 gtcaccgggg ggaaagatgc ccgcatcctg atagattcgg agaaaaccgg taactggttt
      781 gcgctgggca caccgcgcaa cggtcagccg gtactgtttg ccgagggcta tgccactgcg
      841 gcctccctgc acgaagccac gggccttccg gtactgatga cggtggatgg aggcaacatg
      901 attgccgttg ccgaaaatgc ccggcaaaag tggacgcaaa gcccctttat cttctgtgcc
      961 gacaacgatc atgcaatccg ggttaacaaa ggtatcgtca gtgcaacaaa agccgcagaa
     1021 ctgacaggag gatcggtcat ctttcctgcg ttcaccgatg ccgaaaaagc gcaggggctg
     1081 accgatttca atgacctgga cgcaagccgg ggacgggccg cttttcagca tgtcatcaac
     1141 gcccagttag aacacatcgg ggtttccacc cccaacagta acaccccgga aatgcgcgag
     1201 gcactggtga taggcaacct cgtttttaca ccggtccaca ctgaggaaaa aaccatgact
     1261 ccgacagaat atcctgaaac atcacccgat acagggcaca gccatgacca gggaccttct
     1321 ctgccgtccg ccacgcagca ggaacaacca gctgcttcca gcagtaacat tgctgatgaa
     1381 acacagtcat ttaccagtca cgccactgaa aataacggca aagatgagcg gcacgctgat
     1441 aacgttcagg cgcaaacgga aagctctgta gttcaggact cccctgcaga atcaccggcg
     1501 tccccggaaa gtacaacggc gtctgcccct gatgaacccg ctcatccggc ggaacctccg
     1561 gaaaaagttg tctcggtggc cacagataaa gactggcgtg aatttgaagc cgaactgtca
     1621 caaccggaaa aaaacgagtc acaacaggaa tccggcactt cacctgaaat accggcccct
     1681 gctgccgccc ccgcatcccc tgaagacagt ccctcatctg aaaccatgaa tgaatcagcc
     1741 gagccatcac cttcccctga tgcagtcaaa gaggaaatga ccgtgatgac ggagcctgat
     1801 gacaaacaaa gcaccggaat atccccggat actccccggg aagaggcggt caaccatgta
     1861 actgaagaca ttccccttca gccagagccg ttccttcctg agtcagatgg ccccgaagaa
     1921 tacgaagact acagcgcata tcaggagctg atgaacaatg aaaatcctga gcatctgcag
     1981 gaaaacagtg atatgccgtc gcaaccggac acaggccacg ttcaggcaga tgagtcagaa
     2041 cttcaggcca caacagccac tatagaaccg gctgtccggg atgaacccac tccgcagcaa
     2101 cctgttcaga gcacatcacc ttctgataat acttcatcgt ttctcgacaa agcgcgtggc
     2161 ttttttacac gtaaaaaaac ggattcacag gcgcatgaga acagtgatcc cacgccctca
     2221 gcagaaacca cgacgactcc accgatacct gacagtattg tctacgcccc ggaacgttct
     2281 gatgcaccga tatcgctcaa catggacgaa attcttaaag agcttgactg ggaaaaacga
     2341 gcagacagaa ccgtcctgta caaactggat ggcaaacctg ccttcattga ccgggtaaac
     2401 cggctggaaa tggtcaacgg tgccagcaat gacgatcgca gcgttctggc cgcacttgct
     2461 gtggccacca aattttatgg gggcgtcata gagctgacgg gcagtgatgc ctttaagcaa
     2521 aaagcaatgc agttgattat cgagcacaac atcgatgtcc ggatgaaatt gccagatcaa
     2581 cgggctgaac tagaaaaatt acgcaaggaa atggctgtca gtaaggatgc tgttgtcacc
     2641 catcagccaa caccggaact taaccggaat atccctgaac aaccggctgt ccctgacccc
     2701 gttcaggaaa aagaagccac acaatccccg gcgtcccctg ctgcccctga agcatcaacc
     2761 gtcagcaccg ctcctgcatc atcaccggct gaaccaggca aaacggcaga tgcagcacca
     2821 ggtgaagaac caccaggaaa acttcgcccg ggagagagcg tgactgccgt actgcacaat
     2881 tttggtcgtg ctgaatatgc acccggcaaa ggagaaagtt tctttgttga gcttaaaaac
     2941 cgcagtggca gcaaactcta ctggggggaa caactggaaa gtctggtcaa aaaccatcag
     3001 aaaggtgacg tggtgacact gacacttcag aaccgcgaac aattcatcct cccgggagag
     3061 caaaaagcac gtttccggaa taagtggtcc atggagtcag ttaccaacgg gatctcggta
     3121 tcacatgata atccggataa aggtcagcgt attcaggcga ttccggtcga aacttttatg
     3181 aaagtggctg cgcaaatcag ccaggggtgg cccgaagaga taaaagcgtt acgtatgcca
     3241 gaaaacgtcg gctcacatct gtttatcggt gaagaccgac atccggtctc cgcgccacag
     3301 aacgcaaatc aggtaacaga aataaccagc gcagcccctg acaagctgac acctgttctg
     3361 ggatcagtgg ataaggatac gcgtgaactc aacctgctac ttgtccagag tgccgatgaa
     3421 catctgcagg gtgtggtccg gctgaacggc acattgtatc cggcactggc caccccatcg
     3481 gccgacaaca gtcagctggt tatcaatgcg ctgaccgaca aaggtttacg ctttgcaggt
     3541 tatggtgaag cggtaaacca cgatgctgac agcacgaatc gccccgcacc ggagcttatg
     3601 cagtttcatc tgaaaacccg ggaagaaccg ctttttgctg ctgtctacac gccggaaaaa
     3661 caacctgatg cgttgtaccg gaacctgggc ttcgagcagt catggcagca gtggtccaac
     3721 agccaaaagc cggaggacag gcaggaaaaa actctgcatc aggatctctc ccacagcccc
     3781 ggccgatgag ccggtaagga agccatatga acatgcataa gtctctggtt gccgtgctcg
     3841 gccttatcct gatttcatcc acggcaagcg ctgcaacctg tcgtgcgggt agcgcggcac
     3901 aggccggtag taatacagga tatgagcgag cacgtgcagc tgcggatgca tggtcacaac
     3961 gtgaaaacga tgtttcctca tccctgcagt cctgtctgtc acgaatcaaa aaaatcagca
     4021 taaacctccc ccagttcccc agccttgacg acattctgtc gcagctggaa aaccaggtct
     4081 gcgacgcggt cgtggacaag gtgaatgaaa agctgccggg gaatattgat ccctggaagg
     4141 actacaacct atgaccgaaa ataccgagtt atcctcgcca tcacatccgg cacctgaccc
     4201 ggaaatactg aagagcacaa tcagggctat gaagcagtca gaacagcggg caaataatgt
     4261 ccctgcgctg ataaaagcgc ttttatgtac gggcacatgt cttcttatca gtattactgg
     4321 taacgccatt cagtactggc actcaacgaa tgtggagcgc gaatatttcg ctacagacaa
     4381 tggtcgtctg gtcaggctgg caccaacaag tcagccagca tggagtcaga atgatgcaat
     4441 ggcctttggc agccaggcac tggcgaccgc attcaacctg gattttgtgc actatcgttc
     4501 acagatctcc tccctgtctc cgcgtttttc tgacgaaggt tttgtcggat atgtgaatgc
     4561 attacaggca tctaacattc tggagacgat caagaaagag aagatgaatc tgaccgccac
     4621 gacaggtgct ggcgttctgg tacgacaggg acaaatgtcc gacggcgtat ggttctggac
     4681 attccagtat cctgtccgta tgcgtctggt cggccagaca accagcaagc cagaacagtc
     4741 ttttgttttc gaaatcacca tccagcgtgt tgaccccaga ctgaaaccat ccggtatgga
     4801 aatccggcag atgatctccc gtaacgccgg accgaactcc tgagataagt aatatgcaat
     4861 cacgttattt actgagcaca ctgctcctgg tgtgcagcgc cgccacgtct gcagataacg
     4921 caggatggca gaacgcacgc acaccacaaa cgacaaattc tgccagcgat cacgttcaga
     4981 acgcctccca aaacaccgga ggtgttccgg ctacactggt aaaaggtgag ctgcctgccc
     5041 ccgggcaggc cagcccgctg gtacaggatg cggcaaggct tgattctgag ctctccgccg
     5101 atgagattcg ctcactacgc agtctcatgg cggataacga gcgggccatc aatgcgccca
     5161 tcaccagtgt tgtaccccgt atcagctctc tgacagttaa tctgtccccc ggtgccagcc
     5221 ttcctcttgt tcgcacagcc atgaacaacc tgagcgtggt gacatttacc gatatcaacg
     5281 gttcaccatg gccacaatcc gatcctcctt ataacgcggc accaaaactc tttgatgtgc
     5341 agtacaacga gaacatggtg acaatcacac cattgcggcc atgggcatcc ggaaacattt
     5401 ctgtctacct gaaaggtctg tcggtaccgg tcatcctgaa cgtaaccagt ggcgaaacag
     5461 atacgccgtc atccagtcag gaaatggaca gtcgtctgga tctgcgaata ccacggcagg
     5521 gaccaaccag cccggttgtc tccatcccga cggataaaat cgccctgcat gatgcaacat
     5581 tacaggcatt tctggatggt attcctcccc gggacccttc agttaagagg ctgaaattca
     5641 caggtaatgt cccggataca acgatctggc aacacggcga tgacctactg gttcgttccc
     5701 gggcgatgtt gcgcgatgaa tttgaacaga ccttgtcctc tgccgacgga actcatctct
     5761 ggaaacttcc cgtcacccca ctactgactt tctctgtgaa tggccagtcc gttcacgtga
     5821 caccagaact ggagtaatca tgagtgctga acaggatgcc ggtaaatccg gaaagaaact
     5881 ggctgccctg cttggactgg gcagcatcat cttatttggc ggcggctata tcgcgttttc
     5941 caaactatca gggagtaaca gcgatatgca gtcagccgtg aacattaaca gtgcagcaag
     6001 cggcggaacg cgcagcgtta ccgaaacgcc tcactatcgt gaactgttaa gagcagataa
     6061 tgaacgaggg gccgcagctg cagcccgcaa taatcagacg ttcattgcca gtcttccaca
     6121 ggggcttgat attccggact ctcaggaaaa gcaacaacag cctgcagcaa aaccggaaaa
     6181 ttatgctcat cgccaggctt ccggcacacc acaggaggat cgagcagcca gtgaaaaacg
     6241 tatggagcgg ctgcaaaagc tgattgtaag aataaaagat cagcatcctg ccggtagtac
     6301 accaaccata gcaacggcta tgtggaataa atctccggca gaaacaacag ggcagaatgg
     6361 tacgcagcaa ttcgcattac agaatgccag cctgtccacg ccggtagcag aaaaagggat
     6421 acaactgata cctgctctga cccgtatacc ggcatatatt gatactgcag tggattctga
     6481 taaccagtca tccaaggtta tcgcaaccat tccggctggc ccctgggcag gggcaacgct
     6541 gttcagtccg ggagtcaaac tggtgggtaa cggcgtggag atccatttcg accggatgag
     6601 ctggaacggc atggacctta aggtcaatgc atatgctcaa cgggaagaca acctgatgtc
     6661 atccgtggcc agcaatgtga atacacgctg gtttaaacat atcattctgc cgtcagtgtt
     6721 aggaggtgtg ggcagtattg gcacacttta taaagatgcc aacacgcagg ttattcaggg
     6781 taactatggc acggttaccg gacgcgtggg aatgcccagt ggtgaagctg ttgcgggcgt
     6841 tattgccggt ggtatggcgg aaagaggaag tcagatatta acccgccagg cagaaagtga
     6901 gccctataaa caggtagaag tgtatcagca tgaagtcgta tcaattctgt ttgttgaccc
     6961 ggtaatgaca aacgacgcca ggtcatcttc cctttccagt ggcatttccc cttcagttaa
     7021 tcgtacatca cagacggaac aacgctcgca ggcacgaatg cagacagcca tggagcaaag
     7081 aaaagctgtg atgcaacgtc ggtatgatga acaaccggag acaccataat gaaacctgaa
     7141 acagaaatag atgaaaccgg cagcttcggt gaacccgaag agaaacccgc ccctttctgg
     7201 aaacgttccg tctgggggat atctgtagca acatgggggc tatgtgcagt cgtattgctc
     7261 gcagccatat ggtatctgtt cctgagagca ccatcggaaa caggcatgcc tccgtttaat
     7321 gatgcggatg ctggcgtaca gacctggcaa accacacaag aatcatcacc ttccgttcag
     7381 tccactgaga ccatgactgt ccggacagga gaagatatga gccagcttgc ccgggatgta
     7441 aaaacagagc tggataaccg cgatgaaaaa attcaggcaa cactgaatat gcttcacgac
     7501 agcattaaca agctgggcga agctataaaa aaggatgaag aatacgcaca ggaaacgcgc
     7561 cgtcagctgg atgatatccg gagccacctg aacggaatga tgacacagaa aagcgtcacc
     7621 gaatcctctt ccacaccgca tccagcaaag aaaaaaacgt cgtctgtact taacggaatg
     7681 aaaatcatgt cgatggaaac tggcatggtc tggattcgct ggcagggtag tacctgggct
     7741 gtcagagaag gtcagactct gggcaatgtg gtgatccagc ggattgatcc gacgacccgg
     7801 accatcatca ccagtgcagg gacgttacgc tgatgaacat ggatgccctg accgccattg
     7861 agaactttgc cagcagtatt ttcagtgccg gaatggattt cctgtttacc tggggagaat
     7921 tcatcggggt gatcagcatg attaccctgt ttgcccgcgc ccgttcagcc ggtccggtaa
     7981 aaatgtctcc cggaaaattt attgcgggga tgttaaccag ctgcatgctc gtatcactcc
     8041 cggcaatgat taacgccgga ggcgtacaaa tgggattcag ggctgactca tttggcccga
     8101 ttgcatatgt acaacctcag acgtttggcg ctgctgctgg cgcagcaaat gcagttctgt
     8161 cgctggccaa gctggccggt gtgggatttg tcatgaacgg gatatccatc tggcgcaagg
     8221 ccggactcga tggacatacc gcactgagtg ccagtgaatc cgtcagtaaa ggaaacgtca
     8281 aatttattgc cggcgtcctg ctggtcttta tcgatcgtgt cctcaatgcc ctgctggcaa
     8341 gcattggaat cgtcttctga tttttttcac cttcatataa accactgata tatccattta
     8401 aggagaaact atgtcacgta ttcgtgtcgt ggcggcccgt ctgtacgccg catttatcca
     8461 tgcacaactg tttgtaatgt catgccggag caaactgact aagtacctgc tgctcattcc
     8521 catgatgttg ctgcccaaag ccgttcttgc cgatggagat cttgccgata tggttcgcaa
     8581 tgttggtgaa ggtgctaaaa cagcacagtc atcatcaatc actattgcgc agttcatcgg
     8641 cgtaattctg tttctcgggg gcctgattgg cctgaaaaaa gttgggaaac aaggggggat
     8701 gggactggct ggttgcattg tatctatcgt cattggggcg gtacttgtcg ccggacctga
     8761 aatgatgagt cgcagccaga aacagttggg gatttcctca atttccattg gctaatcttg
     8821 tccagggcac tggcatttat ccggtgccct tcctgttcgg caatacaagg aggcacatat
     8881 gtctgcctgg agcccggagc acataacgtt acgacgaaca gaaaacggca atgtgattgc
     8941 ccacgacagt cgggacgatc aggaattcat ttttgcagaa tgctgttccg atgaaatcgc
     9001 aaacatcatt ctggaagccg tgaaacgtta caccagtacg gagcctgctc atgcaacaca
     9061 gcattaaaga cttgtggttg tatccctttc cagaaattga tgtggttcat acacaggagc
     9121 cactgttacc ggaaccggaa ttaacaacgc ccggtcgatg tatctgctgc cggcaaaacg
     9181 tcaggcaccg tttcagactg gatgactcat ggccactcag acagcttacc gacacaatca
     9241 gtgacacacg agttcgtctt aataaggcca cagaacatct ggataagtta aaaaagcgtg
     9301 gtgaaccagt cgcaaccggc gaaaaggaaa agtacaacac ggcagtaaaa gccgctgaac
     9361 gggcgcttga gcaggcccgt ctgtctgcca gacgtctttc actccgccat gtacagaaag
     9421 ctgaaatcac cagtacagag tcgttgtcag agaaagagca ggaactgttt catgaagacg
     9481 gcccgcctta cagcttatgt gctttctgcc acgcctggca ttcactgaat ggttatgccg
     9541 cagcacaggg cgttatggtg tggctacccg accttcatcc atccacggtc gtggccctta
     9601 accgtcgttc attacaggaa gttttcagta atgataagtt ccgggtccgg cggggacggg
     9661 aagcgctctc tgcactgatg caaaacagac tggcggttga ggataaattc cgcagtttcc
     9721 gcccggccga tttcgctgac gtttttcgtc gctatccacc ttccggtcgt tcgcccctgc
     9781 gtgaaaaaat gaacgggatt gccctgattc tgacaccaga ttcattcatc aaaaaagaat
     9841 acgttgattg atttttcttc atgccactcc agcgcggggt ggcattaaaa aaatcaaact
     9901 gcacaaacct ccctcctgta agtccggagt tccctcacct atgaacataa accgtttaat
     9961 cttcactatc gaagactgtc tgaacacgct atcccgtttc tctgtcgcaa gcagttttgt
    10021 tgagtattgc gatttacgta ctgtaattgg tctggacaga caggatcgtg aacgtcgccc
    10081 atggctgaat tcgccctata ttgcagccac aaagcgtgga gaatatctct ctgtctttga
    10141 agtcagcggt gctttcaggg aaatggatga agcatccgac cagactggcc taggttcact
    10201 ggaatcgctg atcaccagca tgagtgacag cctgaatacg gcatataaaa acagcggtca
    10261 taaaatcagc tgcgtttttg agcgtgatcc tgacatgggg aaagaagaaa tcgaagacat
    10321 ggtcgcccca caaaagcgct cactggctaa tacaggtatc cagttacagg atgtggtcga
    10381 tgagaaagtc acaacactgt ccccatggct ggtccgggag cgttgctggc tggcgatctg
    10441 gagtggaccc gaccttatca gcaacagcga tcgcaccgcc catgatgagc ttgtcagaag
    10501 actggctgaa cgggtaccga aagcacgctt tgcccagtcc ccctggcagt ggaccttatc
    10561 tgcactgaaa atccgccacg aagcttttct tgataatgtg gaacaggcac tgagacattc
    10621 atctgatggc ctgattctcc ggctactgga tatacacgag gtcgggcgtg aaatacgtcg
    10681 tcagacagaa cggcacagca caccgcgtaa ctggcaaccc cacctgcctg aagatgccca
    10741 gccagcggga tatcgctgga ctgacgatga atccgtacta catgctcctt cattgcattt
    10801 gcagttattc aacacgcagg tgaccacaca gggcaacctg gttcaggcgg gcggactctg
    10861 gcacggaatg gtatcgatta ctttacctcc gcaaaatctg cagacgttta acgaactggt
    10921 ccgtgccgtc ccccgcgcag tcccctggcg catcagaatg gatctgatgc ccggcgggat
    10981 gaaggctctt aatctgaaga aaacgcttct gacttacagc agcttcattt ctgccgttcg
    11041 tccaatgtat gaatccgtta tgaccctggc ggcaacggat gaaaaagaac ctgtctgcat
    11101 aatgaccatc atggcctcca cgtggggaaa aacacgtgaa atatgcacac gaaaccaggc
    11161 cattctgaaa tcggccattg aaggctgggg cgtatgtggc accacgacaa catttggtga
    11221 tccccgacgc gcctgggtga ataccattct cgcggcatcc ggaggctccg gcccggttcc
    11281 cctgtatccc ccgttgtcac atgccatctc gcttttccca cttaacaggg caggctctgt
    11341 ctggcgaggc aaagggaatc tgatgttaca tacagaagac ggttccgcat ttgaggttgg
    11401 tctggcgtca tcccaacaaa ataaacatac agaactggca ccaggcgatc cgggactggg
    11461 taaatcagtt ctgattaata cgttgtcgga aatacagata tcatctgctc agaaaaacct
    11521 gcccttcatt gcctatatcg ataaaggata cagtgctcag ggactggttc agcttattcg
    11581 cgacagtctt cctccggaaa gaaaagacga agctgtcggt attattctga gcaatgatcc
    11641 ggaatacacc cgtaaccttt ttgacgtgat gtacggcgct aaaaagccta ttacgccgga
    11701 aaagaatttc atgtcatcgg tgctgtgtgc tctctgtgtc gataccggta ccggtcaacc
    11761 ctgtaacccc ggagacaccc gacaaattat caaccagctt atcgagctgg cttttaaaga
    11821 gtacggtgaa aataatccgc gactttatcg cgcctcaacg gaagacctgg tggattcagc
    11881 actgcaggac tcgggactgt atgaaaaaca tgatgcagcc tggtgggcca gatcaacctg
    11941 gttcgaagtc cgggatatgc tccacaatgc cgggtatatc atggcggctc agcgggcaca
    12001 ttatcaggca atgccccagc ttcccgaagt atcctctatg ctgggccata ccagtctcag
    12061 ggatgttttc ggtactgttc agcgtgatgg ttcaaacgaa cttctgctgg actacatccg
    12121 ccgcgccctg gagcagggac ataacgacta ccccatgata tccgggtata cccggtttat
    12181 gatcaacccg gaaacccggg tcatcgctgt cgatctaaat aatgttgccg gtgacaaaac
    12241 gcctgcaggc aggcttaaaa ccggcatcat gtacctgctt gcaggtcaga tcgccggtgg
    12301 tgacttcacc ctgccacaat acagggatga agtgttaaag cagcttccac gggaatacca
    12361 tgaaatcgct cttaaacgta ttaaccaact tgatcaggag gttaagacaa aagtttatga
    12421 tgaactgcat aacgcccggg gcattgactt catctgggaa aatctggata cccaggagcg
    12481 tgagcaacgc aagtttgcta tccgcacggt tctgagtact cagtatctga gggactaccc
    12541 ggaaagtgtt cttaagtcag caaatactct ctggttactg cgctacaaac ccgaagatat
    12601 ccccgtactg agggataact tcaacgtgcc tgaatttatg ctgaaacgct tcctgaaaat
    12661 gccggaaggc ccggcaccgg atggtagtgg tgttcctgtt ctgggagtat tcagggtaaa
    12721 aagtggcacg cttgcgagaa tactcaaatt taccgtcggt ccactggagc tatgggcgct
    12781 gaactcatca ccaaaagaca gtgccctgcg taaaacgctg acaaataagt taggttcagt
    12841 tcgggcacgt aagattctgg ccgaaaactt ccccagaggt tctgcaacgt cattaattga
    12901 gcaccgggcg ggccaacata acagtgacaa tgttattgaa gatctggcct cggagctcat
    12961 ccgaaaacag ggatacaatc tctgatgtgt acaattcata tcaccccacg ctgccggaga
    13021 cctgccagag ggcaggtatt tcttaaaaag cttgaggtaa aaggtgacag tttctcattc
    13081 actctgccgt cacgtctctt caggttacat ccggccccca atcccagagt gatatacgct
    13141 tcggatgcca gaacacaggg aaaggttaca ggcatgcggc tgttgttgcc gtccagcaca
    13201 gatgagctga taacaacggc aatatggcag gtttctgtcc cggatgaagc accattcgac
    13261 gttcagcatg tcatccagtg gatactccct tcaccgctgg cttcgctgat gtccgatgat
    13321 acctggttat ggccatccgt tcccggaacg ccggcactga gtgccggtga ctggcctgtt
    13381 atggatactg acccttccac cttacggcgc gtcagacgtc gttctttcag catcatcgat
    13441 aaccggaaca ggaaaggtta tatcaccagg cgatatcagt gtttcccact ggccccacta
    13501 cctgaagaca gaaaatacga cagccttcct gaccttgtgc tccatcccga gcaggagaca
    13561 ataaaatgca aagaaaaaca ttactcgcag cccttatagc caccctgagt ggaacggcat
    13621 gccaggctca tgcttattca gtcaccgtcg tcgccagccg gcctgttgaa gagcaggtca
    13681 tccccagaat ggaagccatc aaagatgtcc ttggcaacat actgagtacc cagacagcca
    13741 cgggtaccgc tatcaatcag aactctgaaa agctggcaag tgtcattgcc cagaacggac
    13801 aggcgacacg tcagcagatg attttcagta atgaaactca gcgcctcgag gaggcacgga
    13861 aaagcttcac cgtgccagac tccatctgca gtgaatccgc atcagggatt gcaacagaaa
    13921 gcaaaagtgc ctcagcatcc gcagcctcaa aactcagtaa aggtggcggt gtgagcaacc
    13981 gtagcatccg cgaccgtctg gccagcgcag caaactctcc cgtgcgggaa gcttatgatg
    14041 gcgcggccat acacgccagt tactgtacag aagcagaata tgcacgtttt ggaggaaccg
    14101 ccgtttgtcc ttctgtgggc gaaataccag gtggagacag tcaggtacgc tcgatttacc
    14161 acggcgcagg tacagcggac acacctgcag cactgacatg ggatcagaag caaatagatg
    14221 cagcgacggc ttacatgaaa aacacttccc gcccctctgc cggtcgtgct ctgggtaaag
    14281 gagaagtgaa tacccagagt gggcgaacct acgtcggact ccagaacgaa tacaacggca
    14341 tcattgattc tgcgtcgaat ccccaactga cgctgattgc tgacagcacg cctaatgaaa
    14401 gcacacgtaa agcactggct gaaacactgc agtcagactc agcggccgct tacttcgacc
    14461 aggtggcttc accagaagca aaagcacggg gatatatgag tacccgtgag tttgaagctt
    14521 ttgaggctgg ccggcgctac gccaacacag catacctggt ggatttacag gaaatgcagg
    14581 gagataacct gctccgtgaa ctggtaagaa tcactgcgca aatgaactgg caactgaacg
    14641 acctgaaaga acagatccgc cagggtaatg tcatctctgg acagcaactg gctctgaccg
    14701 cccgccagta ttatgaaaaa caactcggta gccttgagaa aacaatcaat caggcaaacg
    14761 cacgttgaaa gctgacgtca gaacaggact gaaaaaatga ataagttacc ggaaaattca
    14821 gaccagacga aaaacgtcaa aaaatcacca tccataaccc gcatagccac ccgggggatt
    14881 attaacattt ttgttcctct gatggagact cgcctgataa cgaattctgt acagcatatg
    14941 ctggaacaac aaaaacgcag gctggacaga tttcgtcagg taaataataa aaaggcgcaa
    15001 ctccacctca cgtgggaaga agctctgcag gccagccata tgtcagtaga cgatctggat
    15061 cgtcgtttcc gccggcgcag aacagtatgg cgattctgtt gctggagtct gctggccatc
    15121 gctttgtttc tcagtggtat gctctttgcc gcctcttcat tacctcttac gactctggta
    15181 cgcgccatca gcactcttgt gttgatactg tcaggcgtcg ctctttgtgc ttccagagca
    15241 ctgattgtta cataccgcct ctggcagttg catgaacgaa aagtcagcga acccgagcag
    15301 gggactttcc gtgattttct gaacgaccgc aatggctggc gcaatgccac attaattgcc
    15361 gtaaccagta aacaatactg atcacgtcca gatgccagta ccactggcat gtcgccatcc
    15421 cccttaatta aatcctgtgt ggcctttgcc catacgcaat tctattccgg agttactgtg
    15481 aaaattttac ttcgcgcact ctgtgcaggc cttgctatat cctcactgcc ggcaatggct
    15541 tcagtgacct atcaggatat tgtgtctgcc gcaaccaatc ctgacgatct gtctcgccag
    15601 gcgctggtca cgatattcgg tgatgtagtc accaatccac tgtcaaccag cgcccccact
    15661 ctgatcggaa gcatgttcgg ggcgttcaac agtatcatcg ccgtacttgc cgttgtctgg
    15721 tttatgttca ttggtatccg tcatgttgtg cgttcggggc atcagggaca ggtattcagc
    15781 accggacgcg atatcgtcgg cacactgagt gtcgttgcag gttttctgat gatcgttcca
    15841 accggaaatg gctggagtat cgctcagctc attatgttat ggggcgcatc gataatgggg
    15901 gttgggtccg ccaatgtgat ggtgcaactt gccgctgata atatcgcaaa tggttattca
    15961 atgactgtac agccagttca ggcatccaca cgaacagcag cgcgtggcat ttttgaaatg
    16021 gaactgtgta agtatgcggt taacgcagga ctaaatgatt ttaatcagac ggtcaaatcc
    16081 tcgacttcac tgatgacaga atcagctaaa accgccagtg gtaattacac tgtcacggta
    16141 tccaatggca gcggtatctg tggttcagcc agcctgtcag tagaaggaaa cggaacaaca
    16201 gatcaatcga cgattggaaa attctttaat cccttcagta aaaacgaata cagcggtgtt
    16261 atctcagcac aacgtgcagc catggataat atgattagcg acatggacaa cgctgccagt
    16321 gagtttgtga caacatttct ggaaaaaaga aattctggca acggaacatt gccggatatc
    16381 gagacacgga ttcagagagc agccgacgag tatgaacgcg ctgtacaaaa atcgctacct
    16441 attgataacg gagaacaatc caggaaagaa gctctcaagt cttatctcac aacctatgga
    16501 tgggtgacgc ttggcgcgtg gtatcagacc tttgccactg ctaaccagcg tctggcagag
    16561 ctggctgaca gagctccagc tgtaacttca atgtcatcac ttggggaagt tggcgatact
    16621 gatttattta gtgctgttat gggggcatac aagactcaat tacagaactc cacatatact
    16681 ccaacgatag gaacgataac aactcaagat gaaagtaatg cagctaatag cacagaccca
    16741 caaagtgtgg tcatgaaaag tatagcgcca accatattaa aatggacaaa ccaagctgct
    16801 actgaatggt caggaacagg aacaaccagc aaccaagtta acccattaat taagatgaaa
    16861 aacatcggcg attatacatt gggtacaaca gaaattctct ggacaggata tacaactgtc
    16921 agagttctcg cgacaatggg tgataactct attttcggaa aggctgttaa tttgttctct
    16981 ggattaccta aaggatttgt tgctctgctg gatgctgcag ctcccccaat atatttcttg
    17041 ttgttcctgt tgttttgtgc gggtttttcc ttatcaattt acctaccatt tataccattc
    17101 attttctgga tgacaggcat tgggaactgg atagtaagcg tattaatagg atgcaccgcc
    17161 gggcctctgt gggcagcaac acatctagga acatcagagg acagaggaag tcgagcagct
    17221 tacggatata tatacttaat tgacagcatg ataagaccac ctctcatggt attcggtttc
    17281 ttttttgcct ctgtcgccat cattgccgtc ggcacaatat taaactcact gtttggcgca
    17341 gcactagtta acgtacaggt aaaatcatta acagggatat tcagccttgc cgggttcctg
    17401 ctaatttacg cccgtatgtg tacaacgtct gtagctgcaa tatttgcttt acaagcatat
    17461 cttcctgacc atgttatcaa tttcttgggt ggtagagatg gtgtaaatac tcttggcaac
    17521 ctcacctcat cagtaaaaga tatttttgca ggttctaata gaaatattag acacagccct
    17581 ggagttagag aggacagact aaaaaatgtc acaaaggatg ataataaaga tggaataaaa
    17641 ggataatttt aataaaacaa ccttgccata aacactcaat cgcattaaaa tatcgatgag
    17701 tgtttatgga gggtttatta tgcaaatggt aaaaacaatc aatagatatc ctgcttggtg
    17761 gacattatat tacgtcctta gatttatata tttcataagc ataccagtgt tatccatttt
    17821 tattcttttc ggagtgctct ccattacatc ttcaagatat gtgacgcaag aagattatat
    17881 ttatacttgc gtctgtctgt ttttactaat tgcccctgca atattgatgt attcccgagc
    17941 aagctcaaga aaagacaaaa taaaaaaaat tgtcgcggag ataaaaaaca ccggttttta
    18001 ttcacctgac aaagaatatg agggactgtc ttttactcag ggcgtctatt ttggcgtgga
    18061 taccaaaaaa ggcactatgc tgtatgctcg tgcttaccct ggaaatataa tggatattat
    18121 cggctttgac attgacaact tcacacgcac tgtgaccgac gccaagacac tagagattta
    18181 tacaaaatac ataaatatac caatggtctc aattccgtca ggatgcatcc atcccaaa
//
LOCUS       DAZPAC010000066        18214 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZPAC010000066 DAZPAC010000000
VERSION     DAZPAC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.066"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            15..1091
                     /locus_tag="ACHUPM_001951"
     CDS             15..1091
                     /locus_tag="ACHUPM_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0869738.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="ACHUPM_001952"
     CDS             complement(1484..1825)
                     /locus_tag="ACHUPM_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR0869739.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHUPM_001953"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHUPM_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFR0869740.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHUPM_001954"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHUPM_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR0869741.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="ACHUPM_001955"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="ACHUPM_001955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR0869742.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /gene="yobB"
                     /locus_tag="ACHUPM_001956"
     CDS             3559..4215
                     /gene="yobB"
                     /locus_tag="ACHUPM_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFR0869743.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="ACHUPM_001957"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="ACHUPM_001957"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR0869744.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHUPM_001958"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHUPM_001958"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR0869745.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="ACHUPM_001959"
     CDS             complement(7167..7826)
                     /locus_tag="ACHUPM_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR0869746.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHUPM_001960"
     CDS             complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHUPM_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR0869747.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHUPM_001961"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHUPM_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFR0869748.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="ACHUPM_001962"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="ACHUPM_001962"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR0869749.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHUPM_001963"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHUPM_001963"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR0869750.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHUPM_001964"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHUPM_001964"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR0869751.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHUPM_001965"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHUPM_001965"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR0869752.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="ACHUPM_001966"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="ACHUPM_001966"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR0869753.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="ACHUPM_001967"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="ACHUPM_001967"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR0869754.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="ACHUPM_001968"
     CDS             complement(17270..18112)
                     /locus_tag="ACHUPM_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFR0869755.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4671 a         4575 c         4392 g         4576 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcaggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggctgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat aaat
//
LOCUS       DAZPAC010000067        18043 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZPAC010000067 DAZPAC010000000
VERSION     DAZPAC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18043
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.067"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(137..682)
                     /gene="syd"
                     /locus_tag="ACHUPM_001969"
     CDS             complement(137..682)
                     /gene="syd"
                     /locus_tag="ACHUPM_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR0869756.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            750..1598
                     /gene="queF"
                     /locus_tag="ACHUPM_001970"
     CDS             750..1598
                     /gene="queF"
                     /locus_tag="ACHUPM_001970"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR0869757.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            1710..3074
                     /gene="ppnN"
                     /locus_tag="ACHUPM_001971"
     CDS             1710..3074
                     /gene="ppnN"
                     /locus_tag="ACHUPM_001971"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR0869758.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            3630..4919
                     /gene="sdaC"
                     /locus_tag="ACHUPM_001972"
     CDS             3630..4919
                     /gene="sdaC"
                     /locus_tag="ACHUPM_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR0869759.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            4977..6344
                     /gene="sdaB"
                     /locus_tag="ACHUPM_001973"
     CDS             4977..6344
                     /gene="sdaB"
                     /locus_tag="ACHUPM_001973"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR0869760.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNASRMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            6456..7211
                     /gene="xni"
                     /locus_tag="ACHUPM_001974"
     CDS             6456..7211
                     /gene="xni"
                     /locus_tag="ACHUPM_001974"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR0869761.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(7316..8464)
                     /gene="fucO"
                     /locus_tag="ACHUPM_001975"
     CDS             complement(7316..8464)
                     /gene="fucO"
                     /locus_tag="ACHUPM_001975"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR0869762.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(8492..9139)
                     /gene="fucA"
                     /locus_tag="ACHUPM_001976"
     CDS             complement(8492..9139)
                     /gene="fucA"
                     /locus_tag="ACHUPM_001976"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR0869763.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            9686..11002
                     /gene="fucP"
                     /locus_tag="ACHUPM_001977"
     CDS             9686..11002
                     /gene="fucP"
                     /locus_tag="ACHUPM_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR0869764.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            11035..12810
                     /gene="fucI"
                     /locus_tag="ACHUPM_001978"
                     /pseudo
     CDS             11035..12810
                     /gene="fucI"
                     /locus_tag="ACHUPM_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            12919..14337
                     /gene="fucK"
                     /locus_tag="ACHUPM_001979"
     CDS             12919..14337
                     /gene="fucK"
                     /locus_tag="ACHUPM_001979"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFR0869765.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            14339..14761
                     /gene="fucU"
                     /locus_tag="ACHUPM_001980"
     CDS             14339..14761
                     /gene="fucU"
                     /locus_tag="ACHUPM_001980"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR0869766.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            14819..15550
                     /gene="fucR"
                     /locus_tag="ACHUPM_001981"
     CDS             14819..15550
                     /gene="fucR"
                     /locus_tag="ACHUPM_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR0869767.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(15594..16694)
                     /gene="rlmM"
                     /locus_tag="ACHUPM_001982"
     CDS             complement(15594..16694)
                     /gene="rlmM"
                     /locus_tag="ACHUPM_001982"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR0869768.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(16687..17082)
                     /locus_tag="ACHUPM_001983"
     CDS             complement(16687..17082)
                     /locus_tag="ACHUPM_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR0869769.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(17101..18018)
                     /gene="gcvA"
                     /locus_tag="ACHUPM_001984"
     CDS             complement(17101..18018)
                     /gene="gcvA"
                     /locus_tag="ACHUPM_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR0869770.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
BASE COUNT         4448 a         4661 c         4387 g         4547 t
ORIGIN      
        1 aagttaagaa ttaacaatca acattttgat tttaaatgaa aataacgatt cttatctttt
       61 taagtattcg cttatttcct ggcgatctcc tacagggtct gtaagagatc tctcacaacc
      121 agatggggta tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg
      181 aggctaaatg cgtccgttta cgtgttccca gcgtctcttt gcacacttca ccggagagat
      241 tacacaccga gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca
      301 gttttagcct tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt
      361 cgtcttcact ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat
      421 ccccggcaaa ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca atatcaaaag
      481 cgcgttcaac cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg
      541 catcttcggt agttgaaata atgcacggtg aaggaacacc gtacagttcc tcacttaacg
      601 gccagctttt atgctcttca tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg
      661 cttgtgcggt caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt
      721 ttagtttatc tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg
      781 cgggcctgac tcttggaaaa tcaaccgatt accgggatac ctatgacgcc agcctactgc
      841 aaggcgttcc acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt
      901 ttcacggtac ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc
      961 cgcaggtcgc tgtcggtcat gttgaacttg attacaccag cgtaaatctg attgagtcga
     1021 agagttttaa gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg
     1081 tgcgccagac gctggagcgc gacttaagca cttgcgctca gggtaagatt agcgtggcgt
     1141 tatatcgtct tgatgaactg gaaggccagc cgataggtca ttttaatggc acttgcattg
     1201 atgaccagga tatcactatc gataactatg aattcactac tgactatctg gagaatgcca
     1261 cctgtggtga aaaagtagtg gaagagacgc ttgtcagcca cctgctgaaa tcaaactgcc
     1321 tgatcaccca tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg
     1381 acagagaaaa actgctgcgt tacctggtct cattccgtca tcacaacgag ttccacgaac
     1441 agtgcgtgga acgcatcttt aatgacctgt tacgcttctg ccagccagaa aaattgagcg
     1501 tttacgcacg ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg
     1561 attttgtccc atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc
     1621 tggattcacg cagaaggttg tgaaaggaca tcaggcaggg ctattgtaat caaagggaat
     1681 gacgatattc gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca
     1741 tggatatgtt gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct
     1801 atcaactgtt tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca
     1861 gcaaagaatt gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg
     1921 gcgtaaagct ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc
     1981 gcgcgttgca ggccaacttg ttcgcggttc tgcgtgacat tctcttcgtt tacgggcaaa
     2041 tccataacac cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc
     2101 tggtcttttc catcttgcgt aacgctcgcg cactgcatgt cggtgaagcg ccaaatatgg
     2161 tggtctgctg gggcggacac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg
     2221 ggaatcagct gggcctgcgt gagctgaata tctgcaccgg ctgtggtccg ggagcgatgg
     2281 aagcgccgat gaaaggtgct gcggtcggac acgcacagca gcgttacaaa gacagtcgtt
     2341 ttattggtat gacagagccg tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg
     2401 aattgatcat catgccagat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg
     2461 gcatcattat cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa
     2521 ttttaatgaa cccggccaac aaagatcagg ttttaccatt gttcctcacc ggcccgaaag
     2581 agagcgccga ctacttccgc gtactggacg agtttgtcgt gcatacgttg ggtgaaaacg
     2641 cgcgccgcca ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa
     2701 aatcgatgcc gctggtgaaa gaaaatcgcc gcgacacagg cgatgcctac agctttaact
     2761 ggtcaatgcg cattgcgcca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg
     2821 ctaatctgaa gctttacccg gatcaacctg ttgaagtgct ggctgccgat ctgcgccgtg
     2881 cgttctccgg tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt
     2941 ttggtcctta caaaatcaac ggcgataaag agattatgcg tcgtatggac gacctgctac
     3001 agggttttgt tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg
     3061 aaatctgcac gtaatctccc tcccccggtg acgtttgttg ccgggttttc tcgtttttgg
     3121 tcacataccc atcaatttat ttcatttgtt atatgaatgt ttcttaccac cctcacgcga
     3181 taaatatcat tgcagttaat atgtcataca atttatgttg taacgcaaac gtttccctat
     3241 tttcataaac cacattttat cgctatgaaa aagaaattat cgccatgatt aactaaaagt
     3301 attgattttt tcagttcaac ctacatatat tgcgcgcccc ggaagaagtc agatgtcgtt
     3361 taatgggcaa atattgccct taaattctct tttacttttg atttacagag taaagcgttg
     3421 ggataatcta tcttccaagt agattattgt atttgggatc aagatcactg atagatacat
     3481 aacttgtgtg tatctttccg ccctcaaatt attacggcgg taaatgatta agccatcgcc
     3541 gatagacaga tttcattttt acggtcaggc accttcccga gctgaactgg ctaaaagctg
     3601 aattatttgc attcctccag gagaaataga tggaaacgac tcaaaccagc acgattgcgt
     3661 cgaaagactc tcgtagtgcc tggcgcaaga cagacaccat gtggatgctg ggcctttacg
     3721 gcacggcaat cggcgcgggc gtgctgttcc tgccaatcaa cgccggtgtt ggcggtatga
     3781 tcccgctgat catcatggct atccttgcgt tcccgatgac attttttgct caccgcggcc
     3841 tgactcgctt cgtactgtct ggtaaaaacc caggcgaaga catcaccgag gttgtagaag
     3901 aacactttgg tattggcgca ggtaaactga ttaccctgct ctacttcttc gctatctacc
     3961 cgatcctgct ggtttatagc gtggcaatca ccaatacagt tgaaagcttc atgtctcacc
     4021 agctgggtat gacaccaccg ccgcgtgcga ttctgtcgct gatcctgatc gtgggtatga
     4081 tgaccatcgt tcgcttcggt gagcagatga tcgttaaagc aatgagtatt ctggtattcc
     4141 cgtttgttgg cgtactgatg ctgctggctc tgtacctgat cccgcagtgg aacggcgcag
     4201 cactggaaac gctgtctctg gacactgcat ctgcaaccgg aaacggtctg tggatgaccc
     4261 tgtggctggc aattccggtt atggtgttct ctttcaacca ctctccgatc atctcttctt
     4321 tcgccgttgc gaagcgtgaa gagtacggcg atatggcaga acagaaatgc tcgaagatcc
     4381 tggcattcgc acacatcatg atggtgctga ccgtaatgtt cttcgtcttc agctgtgtac
     4441 tgagcctgac tccggcagac ctggctgcgg ctaaagagca gaacatctcg attctgtctt
     4501 acctggctaa ccactttaac gcaccggtta tcgcgtggat ggctccgatt atcgcgatta
     4561 tcgctatcac caaatccttc ctcggccact acctgggcgc acgtgaaggc ttcaacggta
     4621 tggtgattaa atctctgcgt ggtaaaggta agtctatcga aatcaacaag ctgaaccgta
     4681 tcactgcgct gttcatgctg gtaacgacct ggattgttgc caccctgaac ccgagcatcc
     4741 tgggtatgat tgaaaccctg ggcggcccaa tcatcgcgat gatcctgttc ctgatgccga
     4801 tgtacgcaat tcagaaagta ccggcaatgc gtaagtacag cggtcacatc agcaacgtat
     4861 tcgttgtcgt gatgggtctg attgcaatct ccgcaatctt ctactctctg ttcagctaag
     4921 tcctttcgcg ccgctttcgg gcggcgcttc ctccgtttta acacgatgta tttcctatga
     4981 ttagcgtatt cgatattttc aaaatcggca ttggcccttc cagttctcat accgttggac
     5041 caatgaaagc gggtaaacaa tttaccgacg atctgattgc ccgtaatctg cttaaagacg
     5101 tgacccgcgt ggtggttgac gtgtacggct cgctctctct gaccggtaaa ggccaccaca
     5161 ctgatatcgc cattattatg ggcctggcgg gtaacctgcc ggataccgtg gatatcgatt
     5221 ccatccctgg ttttattcag gatgtgaata ctcatggtcg cctgatgctg gcaaacggtc
     5281 agcatgaagt ggagttcccg gttgatcagt gcatgaactt ccatgcagac aacctttctc
     5341 tgcatgaaaa cggtatgcgc attaccgcgc tggcgggcga taaagtcgtt tacagccaga
     5401 cttactactc tattggcggt ggctttatcg ttgatgaaga gcattttggc cagcagaata
     5461 gcgcaccggt tgaagttcct tatccgtaca gttcagcagc cgatctgcaa aaacattgtc
     5521 aggaaaccgg gctgtcactc tctggcctga tgatgaaaaa tgagctggcg ctgcacagca
     5581 aagaagagct ggaacagcac ctggcgaacg tatgggaagt catgcgtggc ggtattgagc
     5641 gcggtatttc caccgaaggc gtgttgcctg gcaaactgcg cgttccacgc cgtgctgcgg
     5701 cactacgccg gatgctggtc agccaggata aaaccaccac tgacccgatg gcggttgttg
     5761 actggatcaa catgtttgca ctggcagtga acgaagagaa cgctgctggc ggacgcgtgg
     5821 tgactgcgcc gactaacggt gcgtgcggga ttatcccggc agtgctggcg tactacgaca
     5881 agttcatccg cgaagtgaac gctaactcac tggctcgtta cctgctggta gccagtgcca
     5941 ttggttctct ttataagatg aacgcgtcaa tttctggtgc tgaagtcggc tgccagggtg
     6001 aagttggtgt ggcgtgctca atggcggcgg ctggtctggc agaactgtta ggtgcaagcc
     6061 cggcgcaggt gtgcatcgcg gcggaaatcg ccatggagca caacctcggt ctgacgtgtg
     6121 acccggtcgc cggacaggtc caggtgccat gcatcgagcg taacgccatt gcggcagtaa
     6181 aagcggtgaa cgcctcacgt atggcgctac gccgtaccag cgagccgcgc gtctgcctcg
     6241 ataaagttat cgaaaccatg tacgaaacag gtaaagatat gaacgccaag taccgcgaaa
     6301 cctctcgcgg cggcctggca atgaagatcg ttgcctgcga ttaatcgctc tccagaggcc
     6361 tcgttttgcg aggcctcttc ccgatttctc atccagccgt agcctgttcc ggcatcgaat
     6421 gttaccctta tcgcctgatc tttaaggggg ttatcgtggc tgttcatttg cttattgtcg
     6481 atgcactgaa tcttattcgt cgcattcatg ccgttcaggg atcgccctgt gtcgaaacct
     6541 gccagcatgc gctcgatcag ctcattatgc acagccagcc aacccacgcg gtcgccgttt
     6601 ttgatgatga aaaccgcagt agcggctggc gtcatcagcg tttaccagat tacaaagcgg
     6661 gtcgaccgcc aatgccggaa gagttgcacg acgagatgcc tgcattacgc gccgcctttg
     6721 agcaacgcgg cgtcccgtgc tggtcaacca gcggcaacga agccgatgac ttagccgcca
     6781 cgctggcggt caaagtgaca caggccgggc atcaggcaac gattgtttcg acagataaag
     6841 gctactgtca gttactttca ccgacattac gtattcgtga ttacttccag aaacgttggc
     6901 tggatgcgcc atttatcgat aaagaatttg gcgttcaacc gcagcagttg cccgattact
     6961 ggggacttgc ggggatcagc agttcaaagg taccgggtgt tgcgggaatc ggaccaaaaa
     7021 gcgccacgca gctgctggtc gagtttcaga gtctggaagg gatatatgag aatctggatg
     7081 cggttgccga aaagtggcgc aaaaaattag aaacccataa agagatggcg tttctgtgcc
     7141 gcgatattgc ccgcttacaa accgatttgc atatcgacgg caatttacag caattgcggt
     7201 tggtacggta acggcgagcc ggatgcaaac gattatcaac acattgaatt taatgcactt
     7261 atataccgga taagacgcgg ctagcgtcgt atccggcatt atcacatcag cgcatttacc
     7321 aggcggtatg gtaaagctct acaatatcct caagcgttgc ttcacgcggg ttgccaccgg
     7381 tacaaacatc atccagtgcc gcctgcgcca gtgccggaat gtcttccttg cgtacaccaa
     7441 catcacgcaa atgtggcgga ataccgacat cacggttgag agcaaacacc gcttcaacag
     7501 cggcattacg cgcctcttcc aggctcatac cttccacttt cacgcccata acgcgcgcga
     7561 tatcgcggta cttctcaccg gtaaagtcag cgttatagtg catgacatgc ggtaacagga
     7621 tggcgttcgc aacaccgtgt ggagtgttgt aaaacgcgcc cagtggatgc gccataccat
     7681 gcaccaaccc taacccaaca ttcgagaagc ccatacccgc aacatactgc ccgagcgcca
     7741 tttcttctcc ggcatcctta tcaccagcaa ccgatcctcg cagcgcccca gcaatgattt
     7801 caatcgcttt aatgtgcagt gcatcggtta gcgcccacgc gccacgggta atatacccct
     7861 caatagcatg agtgagcgca tcgacacccg tcgcagcttt cagcgctgga ggcataccat
     7921 ccatcatgtc agcgtcaata aacgccacct gcgggatatc atgcggatca acgcaaacaa
     7981 acttgcgccg tttctcttcg tcagtgatca cgtagttaat ggtcacttct gccgcagtac
     8041 ctgctgtggt aggaattgcc agaatcggta cactgggttt attggtcggg gaaagccctt
     8101 ccaggctacg cacatcggca aactccggat tgttgctgat aatgccaatc gctttacaag
     8161 tatcctgtgg agaaccacca ccaatagcga tcaggtaatc cgcgccgcta ttctggaata
     8221 caccgagccc ttctttgacg acagtaattg ttgggttggg cactacgccg tcgtaaatcg
     8281 cccatgccag ccctgcagca tccatcttat cggtcacttt cgccaccacg ccgcattgca
     8341 ccagcgtttt atcggtgacg atcagcgcct tctgataacc acggcgtttc acctcatcgg
     8401 ttaaagcccc aacagcaccc cgaccaaacc atgccgtttc gttcagaatc attctgttag
     8461 ccatcatcct tctccttgtt gctttacgaa attactcttc aatccgtaac ccataggttt
     8521 tgaatttctc cagcactacg gcaatctctt catcgctcag cactggcacc gggtccgtaa
     8581 tcgccagggt cgccacgtaa agttgcgcca gcacttcaac ttcatgcgcc agccataacg
     8641 ctttttccag attcacctca caagcgataa gcccatgatg ttgtaacaaa gttgccttac
     8701 gatttttgag agccagcgca acatgttcag aaagttcgcg tgttccaaag gtcgcataag
     8761 gcgcgcaagg aatagaatta ccacctgccg ccgcaatcat gtagtgaata gcggggatcg
     8821 gtcggttaag aatggaaact gctgtgcaat gaacggcatg attgtgaaca accgcgttgg
     8881 catccggtct gctttgatag gccgccatat ggaaacgcca ttcgcttgag gggagctttc
     8941 cttcctcatg tttaccgttg ccatcaataa agacaatatg cgactccgtc agtttttcat
     9001 atggaatgcc tgtaggcgta atcagcatcc catcctgata acgtacactg acgttccccg
     9061 ctgtcccctg gttcagtccc aggcgggtca tttccaggca agtgtcaata atctgacgag
     9121 caagtttatt tcgttccatt agctacctct ctctgattca aaacagggca ataatgttgt
     9181 tcctttcaca ctattgaatt agccgtttaa ttacccacca tcttcttcct gattaacaag
     9241 aaagaaattc acaagcttat attttgtgac ctagctcaac taatcacagt aaataactgc
     9301 aagttctctt tttataaccc cattaaaaat gaccgctctt aaaaatattt atcaaaacgg
     9361 tcatttttct attcacccaa gccaggaatg cccgttttcg gcataaacaa ttaatacagt
     9421 catctggttt gtgtttttta tgatttattt tctgaaacgg gcatgaaatt tcgattatta
     9481 aagtgatggt agtcacataa agtcaccttc cagctaataa gtgtgaccgc cgtcatatta
     9541 cagagcgttt tttatttgaa aatgaatcca tgagttcatt tcagacaggc aaatattcac
     9601 tgatatgaag cccgaactcg ctggttttgc acttttgaaa acataaccga ttacgtgctt
     9661 aagcttctga acctaagagg atgctatggg aaacacatca atacaaacgc agagttaccg
     9721 tgcggtagat aaagatgcag ggcaaagcag aagttacatt attccattcg cgctgctgtg
     9781 ctcactgttt tttctttggg cggtagccaa taaccttaac gacattttat tacctcaatt
     9841 ccagcaggct tttacgctga caaatttcca ggctggcctg atccaatcgg ccttttactt
     9901 tggttatttc attatcccaa ttcctgctgg gatattgatg aaaaaactca gttataaagc
     9961 aggaattatt actgggttat ttttatatgc cctgggcgct gcattattct ggcccgctgc
    10021 agaaataatg aactacacct tatttttagt tggcctattt attattgcag ccggattagg
    10081 ttgtctggaa actgccgcaa acccttttgt tacggtatta gggccggaaa gcagtggtca
    10141 cttccgctta aatcttgcgc aaacatttaa ctcgtttggc gcaattatcg cggttgtctt
    10201 tgggcaaaga cttattttgt ctaacgtgcc acatcaatcg caagacgttc tcgataaaat
    10261 gtctccagag caattgagtg cgtataaaca cagcctggta ttatcggtac agacacctta
    10321 tatgatcatc gtggctatcg tgttactggt cgccctgctg atcatgctga cgaaattccc
    10381 ggcattgcag agtgataatc acagtgacgc caaacaagga tcgttctccg catcgctttc
    10441 tcgcctagcg cgtattcgcc actggcgctg ggcagtatta gcgcaattct gctacgttgg
    10501 cgcacaaacg gcctgctgga gctatttgat tcgctacgct gtagaagaaa ttccaggtat
    10561 gactgcaggc tttgccgcta actatttaac cggaaccatg gtgtgcttct ttattggtcg
    10621 tttcaccggt acctggctca tcagtcgctt cgcaccacac aaagtcctgg ccgcctacgc
    10681 attaatcgct atggcactgt gcctgatctc agccttcgct ggcggtcatg tgggcttaat
    10741 agccctgact ttatgcagcg cctttatgtc gattcagtac ccaacaatct tctcgctggg
    10801 cattaagaat ctcggccagg acaccaaata cggttcgtcc ttcatcgtta tgaccattat
    10861 tggcggcggt attgtcactc cggtcatggg ttttgtcagt gacgcggcgg gcaacatccc
    10921 cactgctgaa ctgatcccca cactctgctt cgcggtcatc tttatctttg cccgtttccg
    10981 ctctcaaacg gcaactaact gaacatattt tccgaataaa gtgaggaatc tgtaatgaaa
    11041 aaaatcagct taccgaaaat tggtatccgc ccggttattg acggtcgtcg catgggtgtt
    11101 cgtgagtcgc ttgaagaaca aacaatgaat atggcgaaag ctacggccgc actgctgacc
    11161 gagaaactgc gccatgcctg cggagctgcc gtcgagtgtg tcatttccga tacctgtatc
    11221 gcgggtatgg ctgaagccgc tgcttgcgaa gaaaaattca gcagctagaa tgtaggcctc
    11281 accattacgg taacgccttg ctggtgctat ggcagtgaaa ccatcgacat ggatccaacc
    11341 cgcccgaaag ccatttgggg ctttaacggc actgaacgtc ccggcgctgt ttacctggca
    11401 gcggctctgg cagctcacag ccagaaaggc atcccggcat tctccattta cggtcatgac
    11461 gttcaggatg ccgatgacac atcgattcct gccgatgttg aagaaaaact gctgcgcttt
    11521 gcccgcgccg gtttggccgt cgccagcatg aaaggtaaaa gctatctgtc gctgggcggc
    11581 gtttcgatgg gtatcgccgg ttctattgtt gatcacaact tctttgaatc ctggctggga
    11641 atgaaagtcc aggcggtgga tatgaccgaa ctgcgtcgcc gtatcgatca gaagatttac
    11701 gacgaagccg aactggaaat ggcactgacc tgggctgata aaaacttccg ctatggcgaa
    11761 gatgaaaata acaaacagta tcaacgcaat gccgagcaaa gccgcgcagt tctgcgcgaa
    11821 agtttactga tggcgatgtg tatccgcgac atgatgcaag gcaacagcaa actggccgat
    11881 attggtcgcg tggaagaatc acttggctac aacgccatcg ctgcgggctt ccaggggcaa
    11941 cgtcactgga ccgatcaata tcccaatggt gacaccgccg aagcgatcct caacagttca
    12001 tttgactgga atggcgtgcg cgaacccttt gtcgtggcga ccgaaaacga cagtcttaac
    12061 ggcgtggcaa tgctaatggg tcaccagctc accggcaccg ctcaggtatt tgccgatgtg
    12121 cgtacctact ggtcaccaga agcaattgag cgtgtaacgg ggcataaact cgatggactg
    12181 gcagaacacg gcatcatcca tttgatcaac tcaggttctg ctgcgctgga cggttcctgt
    12241 aaacaacgcg acagcgaagg taacccgacg atgaagccac actgggaaat ctctcagcaa
    12301 gaggctgacg cttgcctcgc cgctaccgaa tggtgcccgg cgatccacga atacttccgt
    12361 ggcggcggtt actcttcccg cttccttacc gaaggcggcg tcccgttcac catgactcgt
    12421 gtcaacatca tcaaaggcct gggatcggta ctgcaaatcg cggaaggctg gagcgtggaa
    12481 ttgccgaagg atgtgcatga catcctcaac aaacgcacca actcaacctg gccaaccacc
    12541 tggtttgcac cgcgcctcac cggtaaaggg ccgtttacgg atgtgtactc ggtaatggcg
    12601 aactggggcg ctaaccatgg ggttctgacc atcggccacg ttggcgcaga ctttatcact
    12661 ctcgcctcca tgctgcgtat cccggtatgt atgcacaacg ttgaagagac caaagtgtat
    12721 cgtccttctg cctgggctgc gcacggcatg gatattgaag gccaggatta ccgcgcttgc
    12781 cagaactacg gtccgttgta caagcgttaa tacattactc cctgatgtga tgcccggtcg
    12841 ctccggctac cgtgcctgaa caagcaagag tggttagccg gataagcaat gttatccggc
    12901 tatattgcag gagcgattat gaaacaagaa gttatcctgg tactcgactg tggcgcgacc
    12961 aatgtcaggg ccatcgcggt taatcggcag ggaaaaattg ttgcccgcgc ctcaacgcct
    13021 aatgccagcg atatcgcgat ggaaaacaac acctggcacc agtggtcttt agacgccatt
    13081 ttgcaacgct ttgctgattg ctgtcggcaa atcaatagtg aactgactga atgccacatc
    13141 cgcggtatcg ccgtcaccac ctttggtgtg gatggcgctc tggtagataa gcaaggcaat
    13201 ctgctctatc cgatcattag ctggaaatgt ccgcgaacag cagcggttat ggacaatatt
    13261 gaacagttga tctccgcaca gcggttgcag gctatttctg gcgtcggagc ctttagtttc
    13321 aatacgttat ataagttggt gtggttgaaa gaaaatcatc cacaactgct ggaacgcgcg
    13381 cacgcctggc tctttatttc gtcgctgatt aaccaccgtt taaccggcga attcactact
    13441 gatatcacga tggccggaac cagccagatg ctggatatcc agcaacgcga tttcagtccg
    13501 caaattttac aagccaccgg tattccacgc cgactcttcc ctcgtctggt ggaagcgggt
    13561 gaacagattg gtacgctaca gaacagcgcc gcagcaatgc tcggcttacc cgttggcata
    13621 ccggtgattt ccgcaggtca cgatacccag ttcgcccttt ttggcgctgg tgccgaccaa
    13681 aatgaacccg tgctctcttc cggtacatgg gaaattttaa tggtccgtag cgcccaggtt
    13741 gatacttcgc tgttaagtca gtacgccgat tctacctgcg aactggatag ccaggcaggg
    13801 ttgtataacc caggtatgca atggctggca tccggcgtgc tggaatgggt gagaaaactg
    13861 ttctggacgg ctgaaacacc ctggcaaatg ttgattgaag aagctcgtct gatcgcacct
    13921 ggcgcggatg gtgtgaaaat gcagtgtgat ttattgtcgt gtcagaacgc tggctggcaa
    13981 ggagtgacgc ttaataccac gcgggggcat ttctatcgcg cggcgctgga agggttaact
    14041 gcgcaattac agcgcaatct acagatgctg gaaaaaatcg ggcactttaa agcctctgaa
    14101 ttattgttag tcggtggagg aagtcgcaac acattgtgga atcagattaa agccaatatg
    14161 cttgatattc cggtaaaagt tctcgacgat gccgaaacga ccgtcgcagg agctgcgctg
    14221 ttcggttggt atggcgtagg ggaatttaac agcccggaag aagcccgtgc acagattcat
    14281 tatcagtacc gttatttcta cccgcaaact gaacctgaat ttatagagga agtgtgaaat
    14341 gctgaaaaca atttcgccgt taatttctcc cgaactattg aaagtgctgg cagagatggg
    14401 acatggagat gaaattattt tttccgatgc tcactttccc gcccattcga tgggaccgca
    14461 ggtgatccgc gctgatggcc tgttggtgag cgacttgctc caagcgatta tcccattatt
    14521 tgagctggac agttatgccc cgccgctggt gatgatggca gcggtagaag gtgacactct
    14581 cgatcctgaa gtagaacgac gttaccgtaa tgcgctttca ctacaagccc cgtgtcctga
    14641 catcatccgc atcaatcgtt ttgcgtttta tgaacgggcg caaaaagcct ttgcgatcgt
    14701 tatcacaggc gaacgagcga agtacgggaa tattctttta aaaaaagggg taacaccgta
    14761 atctcatatc ggtacgcccg catgacgcgg gcggttatcg aatgatgggg tgaaaaatat
    14821 gaaagcggca cgccagcaag cgatagtcga cctgctgctg aaccatacca gcctgaccac
    14881 ggaagctctc tctgaacagc taaaggtcag taaagaaacc attcgtcgcg atctcaatga
    14941 attacagacg cagggtaaaa ttctgcgcaa tcatggacgc gctaaatata tccaccgtca
    15001 aaatcaagac agtggcgatc cctttcacat caggctgaaa agccattatg cgcataaagc
    15061 agatatcgcg cgcgaggcgc tcgcgtggat tgaagaaggg atggtgatag ccttagacgc
    15121 cagttcaact tgctggtatc tggcacgcca gttgcctgac atcaacattc aggtcttcac
    15181 caatagccat ccgatttgcc atgaactcgg taaacgcgaa cgcattcaac tgatcagttc
    15241 cggcggcaca cttgagcgca aatatggctg ttacgtcaat ccctcgctga tttcccaact
    15301 taaatcgctg gaaatcgatc tgtttatttt ttcttgtgaa gggatcgata gcagcggcgc
    15361 actgtgggac tccaatgcga tcaacgctga ttacaaatcg atgctattaa aacgtgccgc
    15421 gcaatcgttg ttattgattg ataaaagtaa atttaatcgt tcaggggaag cccgcatcgg
    15481 gcatctggat gaggtaacgc acattatttc tgatgagcgc caggttgcaa cttctttggt
    15541 aacagcctga cggaaagggt agcaggccga agacgacgcc ccggccttgc ctgttatcgc
    15601 tcgtcgcgac gaccacccac cgcagcccag atgcggcgga cgtgcaccgt cacttcttcg
    15661 cgatcgtgat acaactgccg cgcctgaatc tgagcattta tgccatgttc atcaagctgt
    15721 gcctgaatat acgccagatt gtgtgatact tcttcgtagc gttttttcat cggcagtttg
    15781 agattgaaaa tggtttcacg gcaccagcca ttaaccagcc actgcgccat caatgccgca
    15841 actttagccg gtttttcaac catatcgcat accatccagg agatattgct gcgtgtcgga
    15901 cggaatttga aaccgtcttc ccgcagccac gtcacctgcc cggtatccat cagactttgc
    15961 gccatcgggc cgttgtcgac ggaataaacc cacatgttgc gcttcaccag ttggtaggtc
    16021 cagccgccag ggcaagcgcc taaatccacc gcccacatcc cgttcgccag gcgttcatcc
    16081 cactcatccg caggaataaa cacatgaaat gcctcttcca gtttgagcgt ggaacgactc
    16141 ggcgcctctg ccggaaattt caggcgcgga atgcccatat agaacggcga attattgttg
    16201 ctgtatgagt aaccggtata gcagcagcct ggtgcaataa agaatacatg cacaaccgga
    16261 cgcttcggcg tttcatagtt cgccagcacc ccggcatcgc gcaaggcagc gcgtagcggc
    16321 acggtgaatt tacggcagaa tttcagcaac tctttgcttt cgttggtatc ggcaacttca
    16381 acacgcagtt caccgccctt ctctactacg ccctgcaaca tgtcgacaat gggggtaata
    16441 cgatcttctg gcggcaaatg ctgcaagagt tcccccacca caaaccactg gcgggcaaaa
    16501 attaatgaac tgaacggcag ctcacggatt aacttatcgc catcatcagg ttgataacat
    16561 tcataaatga cataacccgc attctctttc acgcgggcaa aaccgaaaat ttcccgctgg
    16621 ccggctttat cggtaatttc tgcggcgcac tctttttcaa agcccggacg gcacagcaat
    16681 acaaccttat tcatgactta cgcccttgcg ctttaaacgg atagcaccaa ctaacattaa
    16741 cgcccagccc gcgaggaagc tcacgccgcc aaccggtgtg acaaatgccc acaaacgcag
    16801 atgggacagc gccaggcaat aaaggctgcc gctgaacaac accgtgccta acgcgaggaa
    16861 aacgctactc cagtaaaacc agatactgat gcgacgctgc attgccaccg ccagacctaa
    16921 gatcgccagc gtatgaaacg cctggtattc gaggccggtc tggatccagc ccatctcaac
    16981 ggcccccatg gttttactta acacatgcgc gccaaaagcg cccagagcca caaaaatgaa
    17041 gccgctaatg gcggcgaaaa tcagcataaa acggctggtc atggtcgtac cctacgtaaa
    17101 ttattgttca taacgaaagc ggaatttttc ttgttcagca gcggctttcg ccaggatcca
    17161 ttggcgaaag gcggctattt tacccagttc tgcctggctg tcatgacaaa ccagataaaa
    17221 agcgttttta ctgaccagaa catcattaaa cgggcaaaca agacgtccgg cctcgatttc
    17281 agattgcgcc atcacgttat ttgccagcgc cactccctgc ccgtgaatag ccgcttgcag
    17341 caccatggcg ctatgactaa aaattggccc ttgctgaacg ttgatatgat ttaaccccaa
    17401 ctgtcgggta tatgtctgcc agtcacggcg tgacgcatca tgtaataacg tatgtttagc
    17461 cagatcttcc ggggtcttca agggtttttc gcctgtcagc agtagcggcg aacacaccgg
    17521 caataaatat tcggcgtaca gtttttccac ccgtagcccc ggccagttgc cccgaccata
    17581 aaatatcgcc acatcaacat catccgccag cttatcttcc tgacgatcaa ccgcctggat
    17641 tcgaacgtca attcccggat aagctgaatt aaagctggaa agtcgcggaa ccaaccaatg
    17701 aatggcgaaa ctggggagta aactgaccgt caacgccccc ttggcgctac gggcctggag
    17761 tttacgcgtc gcttcggtta attgcgaaaa tatctctttg atatcgagga aatagctttg
    17821 cccttcctcg gtcagcagga gtgaacgatt acggcggcgg aacagtttta gccccaaaaa
    17881 atcctcaaga gacttgattt gatgacttac tgcggcctgg gtcacgaaaa gctcttctgc
    17941 tgcgcgagtg aaacttaaat ggcgtgctgc ggcatcaaaa actcgtaagg catttagcgg
    18001 tggtaatcgt ttagacatgg cttttaaact ttgatgttaa att
//
LOCUS       DAZPAC010000068        17946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZPAC010000068 DAZPAC010000000
VERSION     DAZPAC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.068"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHUPM_001985"
     CDS             complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHUPM_001985"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR0869771.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHUPM_001986"
     CDS             complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHUPM_001986"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR0869772.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHUPM_001987"
     CDS             complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHUPM_001987"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR0869773.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHUPM_001988"
     CDS             complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHUPM_001988"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFR0869774.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHUPM_001989"
     CDS             complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHUPM_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFR0869775.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHUPM_001990"
     CDS             complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHUPM_001990"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR0869776.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHUPM_001991"
     CDS             complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHUPM_001991"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR0869777.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLCLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8450..11206
                     /gene="barA"
                     /locus_tag="ACHUPM_001992"
     CDS             8450..11206
                     /gene="barA"
                     /locus_tag="ACHUPM_001992"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR0869778.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHUPM_001993"
     CDS             complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHUPM_001993"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR0869779.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12799..14139)
                     /locus_tag="ACHUPM_001994"
     CDS             complement(12799..14139)
                     /locus_tag="ACHUPM_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR0869780.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHUPM_001995"
     CDS             complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHUPM_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR0869781.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15928..16377)
                     /locus_tag="ACHUPM_001996"
     CDS             complement(15928..16377)
                     /locus_tag="ACHUPM_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR0869782.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHUPM_001997"
     CDS             complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHUPM_001997"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR0869783.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17177..17506)
                     /locus_tag="ACHUPM_001998"
     CDS             complement(17177..17506)
                     /locus_tag="ACHUPM_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR0869784.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(17753..>17946)
                     /locus_tag="ACHUPM_001999"
     CDS             complement(17753..>17946)
                     /locus_tag="ACHUPM_001999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869785.1"
                     /translation="LREKDDSIFVDRESDNEVNIRMMTLLQDTPGWCFRERLLDEAKR
                     MTQDALKDTSRMENENRSG"
BASE COUNT         4362 a         4904 c         4282 g         4398 t
ORIGIN      
        1 ggaggctggc atttttaaat cagataaagt cagtcttatg cttggccttt gatctcttta
       61 cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga tacgaatcag ctggttgtat
      121 ttagcaacac ggtcagaacg gctcatagaa ccagttttga tctggcctgc agcagtacca
      181 acagccaggt cagcgatggt agcgtcttca gtttcgccag aacggtgaga gataactgca
      241 gtgtagccag catctttcgc catcttgatt gcagccagag tttcggtcag agaaccgatc
      301 tggttgaatt tgatcaggat ggagttagcg ataccttttt cgataccttc tttcaggatc
      361 ttggtgttgg ttacgaacag gtcgtcacca accagctgga ttttgtcgcc cagaactttg
      421 gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat agatacgatc
      481 ggatactgtt tggtcagttc ttccaggaag tgagtgaatt cttcagaggt gaaagctttg
      541 ttgccttcgc cagccagaac gtatttaccg tctttgtaga attcagaagc tgcgcagtcc
      601 atcgccagag tgatgtcttt gcccagttca taaccagcag ctttaacagc ttcagcgata
      661 acagccagag cttcagcgtt ggaacccagg ttcggcgcat agccaccttc gtcaccaact
      721 gcagtgttca tgcctttcgc tttcagaact tttgccaggt gatggaaaac ttcagaaccc
      781 atgcggatgg cttctttcac agttttcgcg ccaaccggct gaatcatgaa ttcctggata
      841 tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg aaccggcatg
      901 gagtatttgc ccggagtgcc gttcagttca gcgatgtgct cgtacagcgg catcccttta
      961 tcggcagcag cagctttggc gttagccaga gatacagcca ggattgcgtt cgcgccgaat
     1021 ttggatttgt tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc agcctgatct
     1081 ttagcgtctt tgccaatcag tgcctgagcg atcgggccgt ttaccgcagc aacagctttg
     1141 gttacgcctt tacccaggaa acgggatttg tcgccatcgc gcagttccag agcttcacgg
     1201 gaaccagtag aagcacctga cggagcagct gccataccga cgaaaccacc ctccagatgt
     1261 acttcggctt caacagtcgg gttaccacgg gagtcgatga tttcacgacc gatgattttt
     1321 acgattttgg acattaggtt ttcctcaagt cactagttaa actgaaactc cagacaaaca
     1381 acgcgtaccc aaggtacgcg ttgccgttct aactttttta cttacttcgc ctgacgtttc
     1441 tggaactcgc tggcagcttt cacaaagcct gcaaacagcg ggtgaccatc acgtggagta
     1501 gaagtaaact ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg aacttcgatg
     1561 atctcgacca actgatcatc cccggaacgg cccgcaacgc gcagacctgc atcttcaatc
     1621 tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac aattgtcggc
     1681 gcattgtaca gctggcgaac caggctatcg tcaaccaact ggcactgctg tgcgccgaga
     1741 cgcatggtac cgccgagatc gctcttctcg ctacgaactt caacgttgcc gttttcatcg
     1801 cgccactcgg taatcagcgc cacaaccggg tacttacagt ctggcacaaa ttccgtagag
     1861 ttggcgttct ccatgttggc aacatggcga gcgtaatcaa ttaacgccac ctgcataccc
     1921 aggcaaatgc ccagataagg aatattgttc tcacgcgcaa aacgcgcggt cgtaatcatg
     1981 ccttctacgc cacgatagcc gaaaccgcca ggtacgagga ttgcgtccag acctttaagg
     2041 atttcaacgc cgcgcgtttc aacatcttgt gaatcgatca gtttgatgtt gacgctgaca
     2101 cgattcttca gcccaccgtg tttcagtgct tcgatcactg atttataagc atccggcagt
     2161 tcaatgtact tgccgaccat accgatggtg acttcactta ccggattcgc ttcttcgaag
     2221 ataacctgtt cccattcgga cagatttgct tccgggcagt ttaagctgaa tcgtttacaa
     2281 atataatcgt ccagcccctg agatttcaac aggcccggaa ttttatagat ggaatcgacg
     2341 tctttcagag aaataaccgc tttttccgga acattacaga acaatgcaat cttcgcacgt
     2401 tcgttcgccg gaacagcgcg atctgaacga caaatcagga tgtcaggctg gataccgatg
     2461 gagagcagct cttttacaga gtgctgagtc ggtttggttt tgacttcacc agacgctgcc
     2521 atgtacggca ccagcgtcag gtgcataaac agagtgtgct cacggccaat ttcaacagcc
     2581 atctggcgaa tcgcttcgag gaacggcaag gattcgatat cacctactgt accgccgatt
     2641 tctaccagta ctacgtcatg accttcgcca ccttccagca cgcgctcttt gattgcgtta
     2701 gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt caccgcggcg ttctttacgc
     2761 agaacgtcag agtagatacg acccgtggtg aagttgttgc ggcggctcat tttggtacga
     2821 atgaaacgct cgtagtgccc caggtccagg tcggtttcag cgccgtcttc agtaacgaac
     2881 acttccccgt gttggattgg gctcatagta cctggatcga cgttgatgta cggatccagt
     2941 ttcatgatgg tcacattgag gccacgggct tcaagaatgg ctgcgaggga ggctgcggca
     3001 atgcctttac ccagagagga tacgaccccg ccggtcacaa aaatatagtt cgttgtcatg
     3061 ctgaacctga gaagttaggt tgaaaagacg atggaataac caggacggga aagcagtata
     3121 cccgaacatg acctgtgcca caaactttca ttatccctcc tcttcgccag cgcactattg
     3181 aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa atcaagcgct
     3241 tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta attcccttag agatcaattt
     3301 cctgccgttt tacctgttgc cagacttctt ccattgtttc gaggtcaaca cctgtcattt
     3361 ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa acgacgctcg aatttttcgt
     3421 tcgctttttg caatgcgatt tctgctttcg tccctaaatg gcgagccaga ttaaccgtgg
     3481 caaacagcag gtcccccatt tcctcctcca gtttagcctg gtcgacaaca gcctgccgcg
     3541 cctcgtacat cacctcgtcg atctcttcgt agactttatc aaccaccggg ccaagcgtcg
     3601 tccagtcgaa gccaacgttg gcgcaacgtt tctggatttt ttgcgcccgc attaaagccg
     3661 gtaaactacg aggaatatcg tccagcgccg aatgctgcgc tttctgcgcg cgctcttcgg
     3721 ttttgatttg ctcccaacgg gcaagcactt cactactgtt ttcggcagaa ctatcagcaa
     3781 aaacatgcgg atggcgacgc tctaatttat cgctaatagc agcgcaaata tcattaaagt
     3841 caaagcgccc ttcttcctga gccatttgcg cgtaaaacac cacctggaat agcagatcgc
     3901 ccagttcacc gcgaagatcg tcaaaatctt cacgggcgat ggcgtccagc acttcgtagg
     3961 tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc cacgggcagc
     4021 cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa acggtcgatt tgattcattg
     4081 aattgtcctg aaaattgcgg gtctgtcagg tggaaacctg tgaccagaat agaagtgagt
     4141 tagtaacact acccaatcag tacgttaatt ttggctttaa tgagttgtaa ttcctctggg
     4201 gcaactgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt tacctgatca
     4261 gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg atatcctttt
     4321 gattgcgttg tacaaggaac acacagacac atacctgttt tgttgttgta catgaaagga
     4381 ctcaggacaa cagctggacg atgtccagct tgctcgctac cttttgtcgg gtcaaaatca
     4441 acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag acttccttat
     4501 ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg tcgttgacca
     4561 gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat ttgccatcca
     4621 ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt aacgtagccg
     4681 ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc ataacccttt
     4741 cctcaaaccg ctatcatatg tagatacagt atatatcaat ctacattgta gatacgagca
     4801 aatttctgcc taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     4861 tttacccagc acgcggccca gcacctgcag gttgtaaatc tcaatggtca tgtcgatggt
     4921 tgccagttgc tgttttgtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     4981 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     5041 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     5101 cgcatgagag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     5161 cccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     5221 gtggtgcatc aggttgccaa caccttcgac taccacgcga ccgttatctt tactgcggtt
     5281 ttgcggcgtg tagctttttt gctgaagttg cttcagcgcg gcggcgtcct gctcttcggc
     5341 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     5401 gccaccaatc gccgccagca actcgtcgac atcattaaag ttgtaacgcg gcagcagatg
     5461 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5521 cccagccaga atgtttttgt cacggtcctg tttacggaac caagcgtgaa ttttcgaacg
     5581 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5641 ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg tgaacggcac
     5701 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5761 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     5821 gacatacacc cggtcgtcaa agacctggct gcgtacttcg tcgagcattt cgccggaatc
     5881 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     5941 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     6001 ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt tgccacctgg
     6061 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacataatc
     6121 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcatagca
     6181 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcaaaggc
     6241 gaggttcttt ttctgcattt tacgccagat gctgtagatg tgtttcggac gaccatacac
     6301 ttccgcttta acgccttcag ctttcatctc agcgcgcaga tgaccaacga actcttcgat
     6361 gtagtgttcg cggtcgagac gccgttcatg cagcagtttg gcaattcgtt tgtattcggt
     6421 tggatggagg taacggaagc agtaatcttc cagttcccat ttcagttgtc cgattccgag
     6481 acggttagcc agcggtgcgt agatgttggt acactctttt gccgccagta cacgttcatc
     6541 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgactac
     6601 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6661 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6721 gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6781 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     6841 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     6901 cccctgcgtc tgttgcagac aatacgccca agtttcggct aagcactcac acgacttctg
     6961 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     7021 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     7081 gcaaatcgca actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     7141 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     7201 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     7261 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     7321 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     7381 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgcc
     7441 ggaacgcctt ctacacccac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7501 cccataccgc agaacaaatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7561 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7621 aaagttaagc acaacccatt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7681 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7741 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7801 gttgcctgta ccagttcaac atgaccaaga tggcgcataa cttgtaagct gcccaagcat
     7861 gccatgactt tgggcagcaa tgcttcaagt tggggcacta aaatggggca ttgtttaacg
     7921 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     7981 aagtagttca gacttaaacg cgcgcgacgg cgatagcccc agggaacatc ggcgatcact
     8041 tcagagactt cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     8101 tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg acagcgtggc
     8161 gtttcgcgtc ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     8221 ttatcttcag taacagtaac ttccgcgttt tcctgcggca ataatccggg gataaatagc
     8281 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     8341 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     8401 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     8461 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8521 gctgagtatc tttttcgttg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8581 cggagccagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8641 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8701 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8761 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     8821 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     8881 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgctggagct
     8941 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcca gcgtgatgat
     9001 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     9061 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     9121 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     9181 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     9241 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttagatc tggcgaaaaa
     9301 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     9361 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     9421 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9481 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc tggaaagtat
     9541 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9601 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9661 cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9721 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9781 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
     9841 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccggtct
     9901 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
     9961 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
    10021 tattatcgaa gggccatcca cccagtgcct cgccggtaag cgcctggcct atgtcgaacc
    10081 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
    10141 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
    10201 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
    10261 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagtcaatg cagaaaaact
    10321 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc gcctgttgcc
    10381 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
    10441 caagctggca atgacagtca tggcagttga tgacaacccc gctaacctga aacttatcgg
    10501 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcggt
    10561 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgcctgacat
    10621 ggatggcatt cgggcctgcg agctcatcca ccagctcccc catcagcaac aaacgccggt
    10681 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10741 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10801 caagcctggc agcggtattt cctctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    10861 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    10921 tctagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    10981 tgaggagcag ctggttggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    11041 tggcagttgc ggctatagcg gtgtgccgcg tatgaagaat ctctgccaac tgatcgaaca
    11101 acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg aactgttgga
    11161 cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga cgtcttgtcg
    11221 agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc ctgtgtttat
    11281 gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc aatgaattaa
    11341 aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc atcagtctca
    11401 agccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg cacgggcgct
    11461 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    11521 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    11581 cacccagccc cggtttttcc ggcacctgaa ccagaccgcc tttgatctca aacggttctt
    11641 tggtcaagcg ctgattgcct tcctgccaaa tccagtgcgt atcaatcgcg gtgattttac
    11701 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgtttg
    11761 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    11821 gcatagtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    11881 ggcccatctg ccgccagtcg gtggcgatca tattggttgc agtcggcagg ccagtcgcgc
    11941 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca tacggatctt
    12001 ctgcataagc cagcgaacct ttcaggtatt taccaatttt aattgcttcg ttcagcgacc
    12061 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa
    12121 tagactcggc ttcttcttcc ccggccagta caccgccttt cagtttgaaa tcgttgaagc
    12181 catatttttc atacgccgct tccgctaggc gcaccaccgc atccggcgtc atcgcttctt
    12241 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagaggtg
    12301 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    12361 gaccatcgcc cagcagcgat gccacgttta cccccagatg ctgccccagc agatccagca
    12421 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    12481 cgcgcccacc agcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    12541 attcacccag cgttttacct accaccagcg gaatcgcatc ttccagcgtt ttacggattt
    12601 tctcgccgcc gggaatttcc cctacgccag tgtgaccgga attatctttg ataatcacaa
    12661 tattacgcgt aaagaacggt gcgtgtgccc cactcagatt catcagcata ctgtcatgac
    12721 ccgccaccgg gatgacctgc atttcagtaa caacagacgt cgtaaattga gaactcatat
    12781 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    12841 gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgccag gcagacgttt
    12901 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    12961 atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca ggcgacaatc
    13021 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    13081 cacatgggta aacatcgcca gagagatatc gaaatggtta ttagaatggc agccccaggt
    13141 cagcccccag tcgtcgcaaa gctgcgccac acggactgca ccggaaagcg tccagaagtg
    13201 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    13261 gttggtggcg atcatgttag tcgcgacggg caagccggtc gcccgccgaa actccgccat
    13321 aacttcacga ccggagaaac cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    13381 atcattcagc cctttgcata gagaaatggc ttcatcaagt agccaggcac cgttgggatc
    13441 aacggtaatc cgcgcatcag ggaagcgttt cttcaatgca cgaacagtgt cgatttcttg
    13501 ctcgccaggt aacaccccgc ccttaagttt gaaatcttta aagccatagc gatcctgtga
    13561 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    13621 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    13681 gatataaaac agataaccga ggacggtaat agtctcgcgt tgcttgcctg gccccaacag
    13741 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    13801 cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg tatcaaaatc
    13861 ggctgcctga ttacctttat gcacctgctg aaccacttta ttcaggcgcg caacttcctg
    13921 gcccagaacc atcggaatag catcgaccag tgtttgataa atcacctctc cgcccggcgc
    13981 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acaatattgc gggtgaaata
    14041 tgcgttatgt gccccaccaa tattaagcaa catgctatcg tgcccggcga ccggaatgac
    14101 tttcatatca gtaataacag gactggattg tgtcgtcatc ttattgcccc gcgacaggtt
    14161 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaagg
    14221 catgaacacc aacataaatc agcgccccat taaaggagcc agtcgtgcca acgatataac
    14281 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    14341 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    14401 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    14461 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    14521 caatgttcag cgatcccgtg cgacgcatca gccagtcgga aataatccca cccagcacac
    14581 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    14641 tcccacgcgc ctgcaccaga taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt
    14701 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agttgtttga
    14761 tctgccccca cttcacgctg aacggcactt taactttggt gttttgctga tccatattga
    14821 tcagcgcgcc acccgcggcg atgtactcca gctctttctg atttaccccc ggatgttgat
    14881 ttggctcgtg gatgactttc aaccagataa agctgatgac aatccccaga ccgcccataa
    14941 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    15001 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgcg
    15061 ccggaaacca ggccgcaaca atgtgactgt tgccggggaa agatggcgct tcagcaagcc
    15121 cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta aagatatcga
    15181 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    15241 ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca taagcccatg
    15301 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    15361 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    15421 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    15481 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    15541 gacatagcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    15601 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    15661 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    15721 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggttaaaacg
    15781 aagaaataaa gaagagtatg aaatggatcg cttgactcca ggcaaacgcc agtaaaaatc
    15841 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    15901 aacgacattt accgctcgct gaacatatca ggacaacagc gtgccccact gttcgaccca
    15961 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16021 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcagaa
    16081 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16141 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16201 ggtcgtggac gtaaccacca gaacatactt atcctgataa ggcagccagt cgcttaattc
    16261 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16321 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16381 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16441 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16501 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16561 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16621 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16681 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16741 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16801 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    16861 tccaccagtg gataatccag caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    16921 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    16981 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17041 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17101 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17161 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17221 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17281 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17341 tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17401 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17461 cttcaagcgt ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacttcaat
    17521 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17581 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17641 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17701 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17761 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    17821 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    17881 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    17941 gaagtc
//
LOCUS       DAZPAC010000069        17938 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZPAC010000069 DAZPAC010000000
VERSION     DAZPAC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17938
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.069"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            211..889
                     /locus_tag="ACHUPM_002000"
                     /pseudo
     CDS             211..889
                     /locus_tag="ACHUPM_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            933..5783
                     /locus_tag="ACHUPM_002001"
     CDS             933..5783
                     /locus_tag="ACHUPM_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFR0869786.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6151..7806
                     /gene="lldP"
                     /locus_tag="ACHUPM_002002"
     CDS             6151..7806
                     /gene="lldP"
                     /locus_tag="ACHUPM_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR0869787.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7806..8582
                     /gene="lldR"
                     /locus_tag="ACHUPM_002003"
     CDS             7806..8582
                     /gene="lldR"
                     /locus_tag="ACHUPM_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR0869788.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8579..9769
                     /gene="lldD"
                     /locus_tag="ACHUPM_002004"
     CDS             8579..9769
                     /gene="lldD"
                     /locus_tag="ACHUPM_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR0869789.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9965..10438
                     /gene="trmL"
                     /locus_tag="ACHUPM_002005"
     CDS             9965..10438
                     /gene="trmL"
                     /locus_tag="ACHUPM_002005"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR0869790.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10491..11312)
                     /gene="cysE"
                     /locus_tag="ACHUPM_002006"
     CDS             complement(10491..11312)
                     /gene="cysE"
                     /locus_tag="ACHUPM_002006"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR0869791.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11392..12411)
                     /gene="gpsA"
                     /locus_tag="ACHUPM_002007"
     CDS             complement(11392..12411)
                     /gene="gpsA"
                     /locus_tag="ACHUPM_002007"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR0869792.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12411..12878)
                     /gene="secB"
                     /locus_tag="ACHUPM_002008"
     CDS             complement(12411..12878)
                     /gene="secB"
                     /locus_tag="ACHUPM_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR0869793.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12941..13192)
                     /gene="grxC"
                     /locus_tag="ACHUPM_002009"
     CDS             complement(12941..13192)
                     /gene="grxC"
                     /locus_tag="ACHUPM_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR0869794.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13334..13765)
                     /locus_tag="ACHUPM_002010"
     CDS             complement(13334..13765)
                     /locus_tag="ACHUPM_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR0869795.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14010..15554
                     /gene="gpmM"
                     /locus_tag="ACHUPM_002011"
                     /gene_synonym="pgmI"
     CDS             14010..15554
                     /gene="gpmM"
                     /locus_tag="ACHUPM_002011"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR0869796.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15588..16847
                     /gene="envC"
                     /locus_tag="ACHUPM_002012"
     CDS             15588..16847
                     /gene="envC"
                     /locus_tag="ACHUPM_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR0869797.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16851..17810
                     /locus_tag="ACHUPM_002013"
     CDS             16851..17810
                     /locus_tag="ACHUPM_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR0869798.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(17797..17938)
                     /locus_tag="ACHUPM_002014"
                     /pseudo
     CDS             complement(17797..17938)
                     /locus_tag="ACHUPM_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4513 a         4638 c         4679 g         4108 t
ORIGIN      
        1 ataatcatca ttagaatcca cgaaaataat atattcatac aaacacagac atcttttgaa
       61 acgaatgata catttttatg atgaatgctt aaaacaatat ttaatcgaca cacatcactg
      121 tcattaatgt aaagcaacat agatcaccaa taatacaatg gacaactcta ccggcattga
      181 tcgctggtcg gaggaaataa ggatttttac atgaacttga agaagatttt tttcagtgct
      241 gtcactgttt cagttctctg cgcattaacg ggatgtgact acattgaaga aggcaagcca
      301 gaaagtagtt tattgaaaca acaagaagaa cattaaaatt gatctacttg aaaaacaaca
      361 agcacagcta aagagtcaac tggaaactat tcaaaaacaa caaactggca taataagcag
      421 tacaaagact ttaactcacg taattaaatc cgttaaagat caacaaaaca cttttatttt
      481 taccgagttt aacccagcaa aaacgaagta tttcatcctg aacaatggtt cggtggcttt
      541 agcgggtcga gtgttatcca ttgacgccac agagaatggc agtgttattc atatttcact
      601 ggtcaactta ttaagtacac ctatctcaaa tattggtttt aatgcgacat ggggtggtga
      661 aaaacctgtc gatgccaaag agtttgcccg gtggcaacaa ttacttttca acacatcaat
      721 gaaatccaca ttgaaattat taccaggtca atggcaagac attaatttga ccctgaaggg
      781 tgtatcgccc aataacctgg gatatctgaa gttagccatc aacatggaaa atattcagtt
      841 cgacaatctt ccctctgctg aaaatcggca gaaaagaagc aaaaaataaa tgcagttagt
      901 acgcttataa caaggaatag cgagcatcat taatgaacaa aatatttaaa gttatctgga
      961 accctgcgac agggaattat actgttacca gcgaaacggc aaaaagccgt ggcaagaaat
     1021 ctgggcgcag taagctgtta atttctgcgc tggttgcggg tggaatgttg tcgtcgtttg
     1081 gggcattggc gaatgccggg aatgacaacg gccagggtgt tgattacggt agtggatcag
     1141 ctggcgacag ctgggttgct ataggcaaag gggcgaaagc aaatactttt atgaacacca
     1201 gtggttccag tactgctgtg ggttatgacg ctatagctga aggccaatat agctctgcca
     1261 tcgggtcaaa aacccatgcg attggtggtg catcaatggc ctttggggtt agtgcaatat
     1321 cagaaggcga tagaagtata gcactgggtg cctcttcgta ttcattgggc caatactcaa
     1381 tggccctcgg ccgttattca aaagcattgg gtaaattgtc tattgctatg ggggactctt
     1441 ccaaagcgga aggagcaaac gccattgccc tgggaaatgc cactaaagct actgagatta
     1501 tgagtattgc tcttggcgac accgccaatg cgtcaaaagc gtattcaatg gcgctgggag
     1561 caagtagcgt cgcatctgaa gaaaacgcaa ttgccctggg gcgtagcagt gtagctagcg
     1621 gtactgacag cctcgcattt ggcagacaat cacttgccag cgcagcgaac gctattgcga
     1681 taggtgctga gaccgaagcc gctgaaaatg caactgctat tggcaataat gcgaaggcaa
     1741 aagggactaa tagcatggca atggggttcg gaagccttgc cgataaagtc aatactatcg
     1801 cattaggaaa tggcagccag gctctggcag ataatgcaat cgccataggc cagggcaaca
     1861 aagctgatgg cgtggatgcc atcgctctgg gtaatggtag ccagtcgaga ggcttaaaca
     1921 ccattgcctt aggcacagcc agtaatgcaa ctggtgataa gagtcttgcg cttggtagta
     1981 atagcagtgc caacggtatt aactctgtcg cgctgggcgc agattccatt gcggatttag
     2041 acaataccgt ctctgtcggc aatagttcat taaaacgcaa gatcgttaat gtgaaaaatg
     2101 gcgcgatcaa gtctgacagt tacgatgcca ttaatggttc acagctttat gccattagcg
     2161 actcggtagc aaaaaggctt ggaggagggg ctgcagtaga tgttgatgac ggtactgtta
     2221 cagcaccaac ctacaattta aaaaatggta gcaaaaataa cgtaggggct gcgctcgctg
     2281 tacttgatga aaacaccctg caatgggacc aaaccaaagg caaatacagc gctgctcatg
     2341 gtactagtag cccaactgcc agcgtaatca ccgatgttgc ggatggcacg atttcagcct
     2401 ccagtaagga tgcggttaac ggttcccaac tgaaagctac caatgacgat gtcgaagcca
     2461 acaccgccaa tatcgctact aataccagca acattgccac gaatacagca agtattgcca
     2521 ccaataccac caatatcacc aacctgacgg attccgttgg tgaccttcag gctgatgccc
     2581 tgctctggaa cgaaactaaa aaggcattca gtgcagctca cggccaggat accaccagca
     2641 aaatcaccaa cgttaaagat gccgacctga cggctgacag cactgatgct gttaacggct
     2701 ctcagctgaa aaccaccaac gatgctgtgg cgacgaatac caccaatatc gccaataaca
     2761 cttccaatat tgccactaac accaccaaca tctctaatct gactgagacg gtgactaatc
     2821 ttggtgagga tgcgctgaaa tgggataagg acaatggtgt attcacggca gctcatggca
     2881 ccgagaccac cagcaaaatc accaacgtta aagatggcga cctgacgact ggcagcaccg
     2941 atgccgttaa cggctctcag ctgaaaacca ccaacgatgc cgtggcgacg aataccacca
     3001 atatcgccac taacaccacc aacatctcta atctgactga gacggtgact aatcttggtg
     3061 aggatgcgct gaaatgggat aaggacaatg gtgtcttcac tgcagctcat ggcaacaata
     3121 ccgccagcaa aatcaccaat atcctggacg gcacagtcac tgcaaccagt tccgatgcca
     3181 ttaacggtag ccagctttat gacttaagca gcaatatcgc cacctacttc ggcggcaatg
     3241 cttctgtgaa tactgacggt gtgtttaccg gtccaaccta caaaatcggt gaaacaaatt
     3301 attataacgt cggcgatgca ctggctgcga ttaactcctc atttagcacg tctctcggcg
     3361 atgctctgct ttgggatgcc accgcaggta aattcagtgc caaacacggt actaatggtg
     3421 acgcaagcgt gatcactgat gtcgcagatg gtgaaatttc agactccagt tctgacgcag
     3481 taaacggctc acaactccac ggcgtgagca gttatgttgt tgatgcgctg gggggtggtg
     3541 ccgaagtcaa tgcagacggc accatcactg cgccgacgta caccattgct aatgctgatt
     3601 acgataatgt cggtgatgcc ctgaatgcta tcgataccac tcttgacgac gctctgctct
     3661 gggatgcgga cgccggtgaa aatggtgcat ttagcgccgc tcacggaaaa gataaaactg
     3721 ccagtgtaat cactaacgtc gctaacggtg caatctctgc tgccagcagc gacgcgatta
     3781 acggctcaca actctatacc accaataagt acatcgctga tgcgctgggt ggtgacgcag
     3841 aagtcaacgc tgacggcacc atcaccgcac cgacttacac cattgcgaac gccgagtaca
     3901 acaacgtcgg tgacgccctg gatgcgcttg atgataacgc cctgctgtgg gatgagactg
     3961 ccaatggcgg tgctggagcc tacaatgcca gccatgacgg taaagccagc atcatcacta
     4021 atgtcgctaa tggcagtatt agtgaggaca gtaccgatgc agtgaacggt tctcagttga
     4081 atgcgacgaa tatgatgatt gagcagaaca cccaaattat caatcagctc gctggtaaca
     4141 ccgacgcaac ctatatcgaa gaaaatggcg ctggtatcaa ctacgttcgt actaacgaca
     4201 acgatttagc ctttaacgat gcaagcgcct ctggtgtcgg cgctacagct gtaggttata
     4261 acgctgtcgc gtctggtgcc agcagcgtag ccattggtca gaacagcagc agcaccgttg
     4321 ataccggtat tgcgctgggt agcagctccg tttccagccg tgtgattgcc aaaggttctc
     4381 gtgacactag cgtaacggaa aatggcgtgg tgattggtta tgacaccact gacggcgaac
     4441 tgctaggtgc attgtcaatt ggtgatgacg gtaaataccg ccaaatcatc aacgtagctg
     4501 atggttcaga agcccatgac gccgttacgg ttcgccagtt gcagaacgct attggagcgg
     4561 tcgccactac gccaaccaag tactttcacg ccaactcaac ggcagaagac tcactggccg
     4621 ttggtgaaga ctcgctggca atgggtgcga aaactgtcgt taatggtaat gcaggtattg
     4681 gtatcggttt gaacactctg gttctagctg atgcgatcaa cggcattgct atcggcagta
     4741 acgcacgcgc aaaccatgca aacagtatcg caatgggtaa cggttctcag accacccgtg
     4801 gtgcacagac tggctacacc gcctacaaca tggacgcacc gcagaactct gtaggtgagt
     4861 tttctgtcgg tagtgaagac ggtcaacgtc agatcacaaa cgtcgcagct ggttcagcgg
     4921 ataccgatgc ggttaacgtg ggtcagttga aagtcactga tgagcgcgta gcgcaaaata
     4981 cccagagcat tactaacctg aacaatcagg tcactaatct ggatactcgc gttactaata
     5041 tcgaaaacgg tattggcgac attgtcacca ccggtagcac caagtacttc aagaccaaca
     5101 ccgatggcgt agatgccaac gcccagggta aagatagcgt tgctattggt tctggttcca
     5161 ttgctgccgc tgacaacagc gtcgcactgg gtaccggttc cgttgcagag gaagaaaata
     5221 caatctctgt aggttcttct actaaccaac gccgtatcac caacgtagct gccggtgtta
     5281 atgccaccga tgcggttaac gtgtcgcagc tgaaatcttc tgaagcaggc ggcgtgcgct
     5341 acgacaccaa agctgatggt tctatcgact atagcaatat caccctcggt ggtggcaacg
     5401 gcagtacgac tcgtatcagc aacgtctccg ctggcgtcaa caacaacgac gcggtgaact
     5461 acgcgcagtt gaagcaaagc gtgcaggaaa cgaagcaata caccgatcag cggatggttg
     5521 agatggataa caaactgtct aaaactgaaa gcaagttgag cggtggtatc gcttctgcaa
     5581 tggcaatgac cggtctgccg caggcttata caccgggtgc cagcatggct tctattggtg
     5641 gcggtactta caacggtgaa tcggcagttg ctttaggtgt atcgatggtg agcgccaatg
     5701 gtcgttgggt ctacaaatta caaggtagta ccaatagcca gggtgaatac tccgccgcac
     5761 tcggtgccgg tattcagtgg taatgatcca ttaagttagt gtgactaagg gcaaacgcca
     5821 aattgcctga tgcgttacgc gcatcaggca agataaggcg ccccgggtca acaatctaag
     5881 ggcaattctc tgatgaggat tgcccttttc tttaccagac atctcccccc acaagaattg
     5941 gccctaccaa ttcttcgctt atctgacctc tggttcacaa ttccccaatt aaaactcaca
     6001 tcaatattgc caatacataa catttagtta accattcatt gtcattatcc ctacacaaca
     6061 caattggcag tgccactttt acacaacgtg tgacaaggag atgagcaaca gactcattac
     6121 acgatgtgcg tggactccag gagacctgca atgaatctct ggcaacaaaa ctacgatccc
     6181 gccgggaata tctggctttc cagtctgata gcatcgcttc ccatcctgtt tttcttcttt
     6241 gcgctgatta agctcaaact gaaaggatac gtcgccgcct catggacggt agcaatcgcc
     6301 cttgccgtgg ctttgctgtt ctataaaatg ccggtcgcta acgcgctggc ctcggtggtt
     6361 tatggcttct tctatggttt gtggcccatc gcgtggatca ttattgcagc agtgttcgtc
     6421 tataagatct cggtgaaaac cgggcagttt gacatcattc gctcgtctat tctttcgata
     6481 acccctgacc agcgtctgca aatgctgatc gtcggtttct gtttcggcgc tttccttgaa
     6541 ggagccgcag gctttggcgc accggtagca attaccgccg cattgctggt cggcctgggt
     6601 tttaaaccgc tgtacgccgc cggactgtgc ctgattgtta acaccgcgcc agtggcattt
     6661 ggcgcgatgg gcattccaat cctggttgcc ggacaagtaa caggcatcga cagctttgag
     6721 attggccaga tggtggggcg gcagctaccg tttatgacca ttatcgtgct gttctggatc
     6781 atggcgatta tggacggctg gcgcggtatc aaggagacgt ggcctgcggt cgtggttgcg
     6841 ggcggctcgt ttgccatcgc tcagtacctt agctctaact tcattgggcc ggagctgccg
     6901 gacattatct cttcgctggt atcactgctc tgcctgacgc tgttcctcaa acgctggcag
     6961 ccagtgcgcg tattccgttt tggtgatttg ggggcgtcaa agattgatat gacgctggcc
     7021 cacaccggtt acactgcggg tcaggtgtta cgtgcctgga caccgttcct gttcctgaca
     7081 gctaccgtaa cactgtggag catcccgccg tttaaagccc tgttcgcatc gggtggtgcg
     7141 ctgtatgagt gggtgatcaa cattccggtg ccgtacctcg ataaactggt tgcccgtatg
     7201 ccaccagtgg tcagcgaggc tacagcctat gccgccgtgt ttaagtttga ctggttctct
     7261 gccaccggca ccgccattct gtttgctgca ctgctctcga ttgtctggct gaagatgaaa
     7321 ccgtctgacg ctatcagcac cttcggcagc acgctgaaag aactggctct acccatctac
     7381 tccatcggca tggtgctggc gttcgccttt atctcgaact attccggact gtcatcaaca
     7441 ctggcactgg cactggcgca caccggtcat gcattcacct tcttctcgcc gttcctcggc
     7501 tggctggggg tattcctgac cgggtcggat acctcatcta acgccctgtt cgccgcgctg
     7561 caagccaccg cagcacaaca aattggcgtc tctgatctgt tgctggttgc cgccaatacc
     7621 accggtggcg tcaccggtaa gatgatctcc ccgcaatcta tcgctatcgc ctgtgcggcg
     7681 gtaggcctgg tgggcaaaga gtctgatttg ttccgcttta ctgtcaaaca cagcctgatc
     7741 ttcacctgta tggtgggcgt gatcaccacg cttcaggctt atgtcttaac gtggatgatt
     7801 ccttaatgat tgttttaccc agacgcctgt cagacgaggt tgccgatcgt gtgcgggcgc
     7861 tgattgatga aaaaaacctg gaagcgggca tgaagttgcc cgctgagcgc caactggcga
     7921 tgcaactcgg cgtatcacgt aattcactgc gcgaggcgct ggcaaaactg gtgagcgaag
     7981 gcgtgctgct cagtcgacgc ggcggcggga cgtttattcg ctggcgtcat gacacatggt
     8041 cggagcaaaa catcgtccag ccgctaaaaa cgctgatggc cgatgatccg gattacagtt
     8101 tcgatattct ggaagcccgc tacgccattg aagccagcac cgcatggcat gcggcaatgc
     8161 gcgccacacc tggcgacaaa gaaaagattc agctttgctt tgaagcaacg ctaagtgaag
     8221 acccggatat cgcctcacaa gcggacgttc gttttcatct ggcgattgcc gaagcctcac
     8281 ataacatcgt gctgctgcaa accatgcgcg gtttcttcga tgtcctgcaa tcctcagtga
     8341 agcatagccg tcagcggatg tatctggtgc caccggtttt ttcacaactg accgaacaac
     8401 atcaggctgt cattgacgcc atttttgctg gtgatgccga cggggcgcgt aaagcaatga
     8461 tggcgcacct tagttttgtt cacaccacca tgaaacgatt cgatgaagat caggctcgcc
     8521 acgcacggat tacccgcctg cccggtgagc ataatgagca ttcgagggag aaaaacgcat
     8581 gattatttcc gcagccagcg attatcgcgc cgcagcgcaa cgcattctgc cgccgttcct
     8641 gttccactat atggatggtg gtgcatattc tgaatacacg ctgcgccgca acgtggaaga
     8701 tttgtcagaa gtggcactgc gccagcgtat tctgaaaaac atgtccgact taagcctgga
     8761 aacgacgctg tttaatgaga aattgtcgat gccggtagca ctggcaccgg tgggtttgtg
     8821 tggcatgtat gcgcgtcgtg gcgaagttca ggcagccaaa gcggcggacg cgcatggcat
     8881 tccgtttact ctctcgacgg tttccgtttg cccgattgaa gaagtcgcgc cagccatcaa
     8941 gcgcccaatg tggttccagc tttatgtact gcgcgatcgc ggctttatgc gtaacgcgct
     9001 ggagcgagca aaagcagcgg gttgttcgac gctggttttc accgtggata tgccgacacc
     9061 gggcgcacgc taccgtgatg cgcattcagg tatgagcggc ccaaatgcgg caatgcgccg
     9121 ctacttgcag gcggtgacgc atccgcaatg ggcgtgggat gtgggcctga acggtcgtcc
     9181 acatgattta ggtaatatct cagcttatct cggcaaaccg accggactgg aagattacat
     9241 cggctggctg gggaataact tcgatccgtc catctcatgg aaagaccttg agtggatccg
     9301 cgatttctgg gatggcccga tggtgatcaa agggatcctc gatccggaag atgcgcgcga
     9361 tgcagtacgt tttggtgctg atggaattgt ggtttctaac cacggtggcc gccagctgga
     9421 cggtgtactc tcttccgctc gtgcgttgcc cgctattgca gatgcggtga aaggtgatat
     9481 cgccattctg gcggatagcg gaattcgtaa cgggcttgat gtcgtgcgta tgattgcgct
     9541 cggtgccgac accgtactgc tgggtcgtgc tttcctgtat gcgctggcaa cagcgggcca
     9601 ggcgggtgta gctaacctgc taaatctgat cgaaaaagag atgaaagtgg cgatgacgct
     9661 gactggcgcg aaatcgatca gtgaaattac gcaagattcg ctggtgcagg ggctgggtaa
     9721 agagttgcct acggcactgg ctccgatggc gaaagggaat gcggcatagt cgtttgcgcc
     9781 ccctcaccct aaccctctcc ctcagggaga ggggaccgtt cggcgctgta tgtactccct
     9841 cactctgaaa cgacaccgca ctcttttttt ctccctcgcc cctccgggga gggccggggt
     9901 gaggggaaaa ggccgcacgg ttcaccaatt ttctgctatc ctgcccccgc actaaggggg
     9961 cagtatgcta aacatcgtac tttacgaacc agaaattccg ccaaatactg gcaacatcat
    10021 ccgtctttgc gctaataccg gctttcgtct gcatatcatc gaaccgatgg gatttgcctg
    10081 ggacgataag cgcctgcgcc gcgcggggct ggactatcac gagtttaccg ccgttacgcg
    10141 tcatcatgac tatcgcgcgt tcctcgaagc agaaaatccc cagcgcctgt ttgccctcac
    10201 cacgaaaggt acgcctgctc acagcgccgt aagctatcag gatggcgatt atctgatgtt
    10261 cggcccggaa acacgcggcc tgccagcgag cattcttgat gccctgcccg ctgaacaaaa
    10321 aattcgcatt ccgatggtgc cggacagccg cagcatgaat ctgtccaatg cggtgtcggt
    10381 agtggtgtat gaagcctggc ggcagctggg atatccggga gcgttgttga gagattagtt
    10441 actgtatgcc ggatagatga ttacatcgca tccggcacga tcacaggaca ttagatccca
    10501 tccccatact caaatgtatg gttaataccg ttgaaatgct ggtccatatc cattgatggc
    10561 ttatcgctgt ctggtttacc gacaatacga gccggaacgc cagcggcggt tgtatgcggc
    10621 ggcaccggtt gcagcaccac ggaacctgcg ccaatcttcg cgccgcgacc aacttcaata
    10681 ttgccgagga ttttcgcgcc cgcgccaatc atcacacctt cacgaatttt cgggtgacgg
    10741 tcaccaccag atttacccgt accgccaagc gtcacagatt gcagaatcga tacgtcgttt
    10801 tcaattaccg ccgtttcacc cacaacgatg cccgtggcgt ggtcgagcat aatgccacgg
    10861 ccaatttttg ctgccgggtg aatatcgacc tggaacgtca cagaaacctg gttttgcaga
    10921 aagattgcca atgcgcgacg cccctgattc cacaaccagt gaccgatgcg ataggcctgc
    10981 aaggcatgaa aacccttcag gtataacaac ggggttgagt atttatcgac tgccgggtcg
    11041 cgggtacgca ccgcctgaat atcacaggcc gcagaggcga tcatttccgg gtcagcggcg
    11101 taggcttctt ccaccacttc acggatagca atagcaggca taattggcga tgacagcttg
    11161 ttcgccagca tgtagctcag tgcactacca aggttttcgt gcttgagtag cgtcgcgtgg
    11221 taaaaactgg ccagcattgg ctcacagtcc gccagcgttc tggcttcggc tttaatattg
    11281 ttccagacaa tttccagttc ttcacacgac attgcttact ccacacgatg agataatgac
    11341 cggcccgttc tgcgcgggcc gggtcatagc ggtaacaaag gttccctggg gttagtggct
    11401 gctgcgctcg tccttgcgtg cacgacctaa taacgtcaat gctgcctcgc gcgcgttttt
    11461 tccgcaatat aatacttgat aaatttcctc ggttattggc atttctacgc cgaagcgatg
    11521 cgccagttcg cggacttctt tcgtattgcg gtagccttcc accacctgac caatcttctc
    11581 ctgcgcgctt tgtacatcca tgccctgacc gagcatcatg ccaaaacggc ggttacgcga
    11641 ctggttgtcg gtacaggtaa gcaccagatc gcccagcccc gccatgccca taaaggtggc
    11701 aggatcggca cccagcgccg cgccaagacg cgacatttca gccagcccac gggtgatcag
    11761 tgccgtacgc gcattcgcac caaaaccgat accgtcggac atccccgcgc caatggcaat
    11821 gacgtttttc accgcaccgc caagctgcac accgatgaaa tccgggttgc tgtaaacgcg
    11881 gaaacttttg ccacagtgca gcaattgttg gagatcatcg gcaaaggtct ggtcggtaga
    11941 ggccaacgaa atagccgtcg gtaaacctgc cgccagttct ttcgcaaacg ttgggccaga
    12001 gataaccgcc agcggaattt gatcgcctaa ggcctcacgc gccacgtcct gtaacagacg
    12061 tccggtttcc gcttccagcc ctttggtcgc ccacaccaga cgcgcatcag gacgcatcag
    12121 cggtttaatc tggcgcagca cttcaccaaa gacatggctg ggtacgacga cgagaatatt
    12181 acggctggct gccagcgcag tggcgagatc gctttcaaga tggagcgtat cgggaaaagg
    12241 cacatcgggg agaaacgcgg cgttacagcg gtcgcgttca agcgttgcga tatgttcagg
    12301 gtcatggccc cagaggacaa cctcgtggcc atttcttgcc agggtaatgg caagagcggt
    12361 gccgtacgag ccggcaccga tcacagtcat tgaagcatta cgttggttca tcaggcatcc
    12421 tgatgttctt cagtaccttc gccagcctgc tgctgcaaat agttcatgaa cagcgcatcg
    12481 aagttaatcg gcgcaaggtt cagttgcggg aatgtaccgc gggataccac gctggtgatg
    12541 cactcacgag catacgggaa cagaatgttc gggcagtatg cgcccaggca atgtgccatc
    12601 tgggtgcctt cgatacccgc gatggagaaa ataccgccct gctgaacttc acacaggaac
    12661 gcggtttctt cgcccaggga ggccgttacg gtaacacgca gtaccacttc gtatacgtca
    12721 tctgccagtt gggaagatgc cgtatccaga tcaagtttaa cttctggttg ccaatctttc
    12781 tggaaaacgt gcggcgcgtt cggcgcttcg aaagagatat ccttggtata aatacgttgg
    12841 atctggaaag tcatttcagt gttgttttgt tctgacatgt gtagaaaacc cttaagtgtt
    12901 gtccttaaat actgcgcaat gtaatgccaa cgttcacacg ttatttcagc aggggatcca
    12961 gtccaccacg tgcatccaat gcatacaagt catcacagcc gccaatgtgc tgtgcgtcaa
    13021 taaaaatctg gggaaccgtg gtgcgaccgc tgcgtttgat catctcttca cgcttggcgg
    13081 cattgccatc gatcggcaac tcctgaaaac tcacgccctt gctgctcagc agtgcttttg
    13141 cacgatggca atacgggcag gtttctttgg tatagatttc aacattggcc ataatttagc
    13201 tcctgttttt ttaccctgaa atttcatgtt gcagggcggc tgcaaaatgg agtgtcccca
    13261 gaagcttata acaataagtc gctgggggcc taattgcagc taacggcctg catcatgaaa
    13321 gacgacaggt aaattatttg ccgcgcacca gaggcaagtt ttcgccagcc cagccagcaa
    13381 cgccctcttt cagcacgaat acttgcgcaa aaccagcttt cgtcagcgcg tttgcaggct
    13441 cctggcactg catgccagaa ccgtctacca cgataaccgg tttgtctttg tgcttctcaa
    13501 gctcaccaac attgttggct ttgatttcgc tcggcaacag attgatagaa cctgcgatat
    13561 ggcctttacg gaagtcatca cgctgacgta aatccacaac cacagcgtct tctttgttga
    13621 tcagacgcgt agcttcacca cgagtaatca ccttcacttt cgaggtcagg ctcttaaacg
    13681 tagtcacaag aaccgccacc agtaacgcga tccaggcgat actcagtatg ggatgacggc
    13741 caacaaattg cataatttct tgcatggggg gtaacaactc ccgacgtagt gattaaaaaa
    13801 accaggaaag gagtatacct gcgcggtgcg gcaaatacag ccagcgcgtt aactggaatg
    13861 caattttgcg gggcacgacg aaaaaaaagc gcaaatctgg catcggcttg ccgcgagcgg
    13921 tcgtatattt tgatcttcag aggctatttt atcgattcag ctgtagtaaa attacgcaaa
    13981 ttttgactct tgagtatgag gttgtcgcaa tgtcggtttc taaaaaacct atggtactgg
    14041 tgattctgga tggatatggc tatcgtgaag aacagcagga taacgccatt tttagtgcta
    14101 aaaccccggt aatggatgca ctgtgggcca atcgtccgca taccctgatc gacgcttccg
    14161 gtctggaagt cggtctgcct gaccgtcaga tgggtaactc cgaagtaggc cacgttaacc
    14221 tgggtgccgg ccgcatcgtg tatcaggacc tgactcgtct ggacgttgaa atcaaagatc
    14281 gcgctttctt tgctaatcca gtgctgactg gcgcggtaga taaagcgaaa aacgcaggta
    14341 aagcagtaca cattatgggt ctgctctccg caggcggcgt acacagccac gaagatcaca
    14401 tcatggcgat ggtagaactg gcagctgaac gcggcgcaga aaaaatctac ctgcacgcat
    14461 tccttgacgg tcgcgacacc ccgccgcgca gtgctgaatc ctcgctgaaa aaatttgaag
    14521 agaaatttgc tgcgctgggt aaaggccgcg tagcgtccat cattggtcgt tactacgcga
    14581 tggaccgcga taaccgttgg gatcgcgttg aaaaagccta tgacctgctg actctggcgc
    14641 agggtgagtt tcaggccgat actgccgttg ctggtttgca ggcagcttat gctcgcgacg
    14701 aaaacgacga gttcgtgaaa gcgaccgtta ttcgtgcaga aggtcagcca gatgcggcaa
    14761 tggaagacgg cgatgcgctg attttcatga acttccgtgc tgaccgcgcg cgtgaaatca
    14821 ctcgtgcatt cgtgaacgct gatttcgatg gcttcgcgcg taagaaagtg gttaacgtcg
    14881 atttcgtgat gctgaccgaa tacgccgctg acatcaaaac tgcggttgct tacccacccg
    14941 cttccctggt taacaccttc ggcgagtgga tggcgaaaaa cgacaaaact cagttgcgta
    15001 tttccgaaac cgaaaaatat gcccacgtta ctttcttctt caacggtggc gtagaagagt
    15061 cgttcaaagg cgaagatcgc attctgatca actcaccgaa agtggctacc tacgatctgc
    15121 aaccggaaat gagctccgca gagctgaccg aaaaactggt tgcggccatc aagagcggca
    15181 aatacgacac cattatctgt aactatccga acggcgacat ggtaggtcac accggggtga
    15241 tggaagcggc ggttaaagcg gttgaagcac tggatcactg cgtggaagaa gtcgcgaaag
    15301 cggttgagtc cgtgggtgga caactgctga tcaccgctga ccacggtaac gctgaacaga
    15361 tgcgcgatcc ggcaacgggt caggcacaca cggcacacac caacctgcca gttccgctga
    15421 tttacgttgg tgataagaac gtgaaagcgg ttgcaggcgg caaactttct gacatcgcgc
    15481 cgaccatgtt gtcgctgatg ggtatggaaa tcccgcaaga gatgactggt aagccgctgt
    15541 tcatcgtgga ataatccctc cccatgaggg gaaaggcgat taataccatg acacgggccg
    15601 tgaaaccgcg caggtttgca atcaggccca tcatctacgc cagcgttctt agcgctggcg
    15661 tattgttgtg cgccttttcc gcccacgcgg atgagcgtga ccaactcaaa tctattcagg
    15721 ccgatatcgc cacaaaagag cgcgcggtac gccaaaagca acaacaacgc gcaagcctgc
    15781 tcgcacaatt gaaaaagcag gaagaagcga tctctgaagc cacccgtaag ctgcgcgaaa
    15841 cgcaaaacac gctcaatcaa ctcaataaac agattgatga gatgaacgcg tcgattgcca
    15901 aactggagca gcaaaaagcc gcccaggagc gcagcctcgc cgcacaactg gatgccgcat
    15961 tccgtcaggg cgagcatacc ggtattcagc taattctcag cggtgaagaa agccagcgtg
    16021 gacagcgttt acaggcttat ttcggctatc tcaaccaggc gcgacaagaa accattgccc
    16081 agttgaagca aacgcgtgaa gaagtcgcca tgcagcgtgc tgaactggaa gagaaacaaa
    16141 gcgagcaaca aacgctgtta tatgaacagc gcgcccaaca ggcgaaactg actcaggctc
    16201 tgaacgagcg taaaaagacg ctggcggggc tggaatcttc catccagcaa ggtcagcagc
    16261 agctaagtga actgcgtgcc aacgaatccc gtctgcgtaa cagtattgcc cgtgcggaag
    16321 ccgcggcgaa agcgcgtgct gaacgagaag cgcgtgaggc tcaggcagtt cgcgacaggc
    16381 agaaagaagc gacgcgcaaa ggcaccacct acaaaccgac cgaaagcgaa aaatcgctga
    16441 tgtcccgaac tggtggcctg ggtgcgccgc gtggtcaggc attctggcca gttcgtggcc
    16501 ctacgctgca tcgctatggc gagcagctac agggtgaatt acgctggaaa ggaatggtta
    16561 tcggtgcctc tgaaggtact gaagttaaag caattgccga cggtcgggtg attctggctg
    16621 actggctgca aggctacggt ctggtggtgg tggttgagca cggtaaaggc gatatgagtc
    16681 tttacggcta taatcaaagc gcgctggtga gcgttggttc gcaggttcgt gcgggccagc
    16741 caattgcact ggtgggcagc agtggcggtc agggcagacc ttcactctat ttcgaaattc
    16801 gccgccaggg tcaggccgtc aatccacagc cgtggttggg aagataagtt ttgtttccat
    16861 ttcgtcgtaa cgttcttgca tttgccgctc tgttggcgct ctcctcccct gtaattgctg
    16921 gcaaacttgc catcgtcatt gatgattttg ggtatcgccc gaacaacgaa aaccaggtgc
    16981 tggcgatgcc ttccgctatc tccgtcgctg tattacccga ttcaccgcac gccagagaga
    17041 tggcgaccaa agcgcataac agcggtcacg aagtgttgat tcatctcccg atggcaccgt
    17101 tgagtaaaca gccgctggag aaaaatacgc tgcgcccgga gatgagcagc gacgaaattg
    17161 agcgcattat tcgtagtgcg gtcaataacg tgccctatgc cgtggggatc aataaccaca
    17221 tgggcagcaa gatgacctct aacctgtttg gtatgcagaa agtgatgcag gcgctggagc
    17281 gttataatct ttacttcctc gacagcgtaa ccattggtaa tacccaggcg atgcgcgccg
    17341 cgcaaggcac tggcgtgaag gtgatcaaac ggaaggtgtt cctcgacgat tcacagaacg
    17401 aagcggacat ccgtgtgcaa ttcaatcgcg caattgacct agcgcgtcgc aacggatcaa
    17461 ccattgccat tgggcatcct cacccttcaa cggtacgcgt gttgcaacag atggtttata
    17521 acctgccgcc agacattacg ctggtgaaag ccagcagctt gctgaatgaa ccgcaggttg
    17581 atacgtctac accgccgaaa aacactgtgc ccgacacacc gcgtaatccg ttccgtggcg
    17641 tgaagctgtg caaaccgaag aaaccgatag aacctgttta tgctaatcgc ttctttgaag
    17701 tattaagcga aagcatcagc cagagcacgc tgatcgttta cttccagcat cagtggcaag
    17761 gctggggcaa acagcctgaa gcggcgaagc ttaacgctag cgtaaattaa aggcgctacg
    17821 cgtaatgcga tggtgcgacc gcgttttgtc gcgccattgc cataagcgga aagactacag
    17881 taacgcctga taacccacct ttacgctgcg cacattggta atcaggcgct tatacata
//
LOCUS       DAZPAC010000070        17852 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZPAC010000070 DAZPAC010000000
VERSION     DAZPAC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.070"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHUPM_002015"
     CDS             complement(<1..304)
                     /locus_tag="ACHUPM_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR0869799.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(514..1032)
                     /locus_tag="ACHUPM_002016"
     CDS             complement(514..1032)
                     /locus_tag="ACHUPM_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR0869800.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1729..2229
                     /gene="tssB"
                     /locus_tag="ACHUPM_002017"
     CDS             1729..2229
                     /gene="tssB"
                     /locus_tag="ACHUPM_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFR0869801.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            2264..2488
                     /locus_tag="ACHUPM_002018"
     CDS             2264..2488
                     /locus_tag="ACHUPM_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869802.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            2539..4014
                     /gene="tssC"
                     /locus_tag="ACHUPM_002019"
                     /pseudo
     CDS             2539..4014
                     /gene="tssC"
                     /locus_tag="ACHUPM_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            4021..4434
                     /gene="tssE"
                     /locus_tag="ACHUPM_002020"
     CDS             4021..4434
                     /gene="tssE"
                     /locus_tag="ACHUPM_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR0869803.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            4438..6288
                     /gene="tssF"
                     /locus_tag="ACHUPM_002021"
     CDS             4438..6288
                     /gene="tssF"
                     /locus_tag="ACHUPM_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR0869804.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            6252..7334
                     /gene="tssG"
                     /locus_tag="ACHUPM_002022"
     CDS             6252..7334
                     /gene="tssG"
                     /locus_tag="ACHUPM_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFR0869805.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            7359..8639
                     /gene="tagH"
                     /locus_tag="ACHUPM_002023"
     CDS             7359..8639
                     /gene="tagH"
                     /locus_tag="ACHUPM_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFR0869806.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            8636..9160
                     /gene="tssJ"
                     /locus_tag="ACHUPM_002024"
     CDS             8636..9160
                     /gene="tssJ"
                     /locus_tag="ACHUPM_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFR0869807.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            9163..10492
                     /gene="tssK"
                     /locus_tag="ACHUPM_002025"
                     /pseudo
     CDS             9163..10492
                     /gene="tssK"
                     /locus_tag="ACHUPM_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            10497..11258
                     /gene="icmH"
                     /locus_tag="ACHUPM_002026"
     CDS             10497..11258
                     /gene="icmH"
                     /locus_tag="ACHUPM_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFR0869808.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            11267..14050
                     /gene="tssH"
                     /locus_tag="ACHUPM_002027"
                     /gene_synonym="clpV"
                     /pseudo
     CDS             11267..14050
                     /gene="tssH"
                     /locus_tag="ACHUPM_002027"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            14047..14790
                     /gene="vasI"
                     /locus_tag="ACHUPM_002028"
     CDS             14047..14790
                     /gene="vasI"
                     /locus_tag="ACHUPM_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFR0869809.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            14795..16207
                     /gene="tssA"
                     /locus_tag="ACHUPM_002029"
     CDS             14795..16207
                     /gene="tssA"
                     /locus_tag="ACHUPM_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFR0869810.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            16226..>17852
                     /gene="tssM"
                     /locus_tag="ACHUPM_002030"
     CDS             16226..>17852
                     /gene="tssM"
                     /locus_tag="ACHUPM_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFR0869811.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPY"
BASE COUNT         4155 a         4894 c         4877 g         3926 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 tcgtcaggta ggccattttg tccagtacct gcggaaattc aagggagaga aactgctggc
      181 tgaccagaga gagctcgagg gaaaagagcg aagagagtga ctggttcaga tgaaaggaga
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag cgtgaaacgt aatccggttg
      301 acatagcaac ctccggggtt aaggaaaaac agacagttcg ctgttaaagc ggctgtgaca
      361 gtacagtgtt acgcttgcgt tcaggcacgc taccgccgaa gtttccggca tccgccatct
      421 cccgtgaacg taacccctta ctgacaacct ttctttctac ggcgtaatac aaaaacagca
      481 tccggcctga tagccggatg ctgttggctt aacttatgct tccagtggtg cgcgccagtc
      541 gtcggcaccg gaggtgccag atttgacgtg ttcccattca attttgcggt aggccagtga
      601 aacctcgagc aactgggtga actcgcgttt cgccgggtcc tggcaatgcg gcatatgcag
      661 tttcatatcg acaatcgtcg aatcagtcag gcgggtagtg aaataatgct cctgtttgcc
      721 ctccacagag gtacgccacc agtgaagttc agtggttggc agcatttcgc cggaggccag
      781 tgcgttgtac atcagcggaa ccgctttgtt cagcgccacg gtaaaaatga aaggcttgtg
      841 agcacgctga cccgcaggct gaccggactg aggagtggtc gggacagtaa cgttatgcag
      901 gaattcctgc accagcatct cgtcttcgtg cccctgcaca tagatgttgc cgacagaatc
      961 agcggtaaat gcacctgcag taatatttcc ctgggtctga ccagtgatgg agatataaca
     1021 cggtgttggc atttgtaaac tccttgttaa cgtggttaat tactacttac aatgacctgc
     1081 cgttaatccc agacaggcag atgcagctca cttcacggaa ccgcatcatt tttcagtatt
     1141 ttttacatac aaaccgagct agaatttacg gttgtcgtaa aaactatttt taaaatcatt
     1201 aaatctctta ttcagcaata acttaataat atatatctgg ataaccatac ccaactgttc
     1261 aaagtccgta ttgcctccat aaaatggcca aagctgaatc atgaataaat attgtattta
     1321 tcctttctga attatcattt tgataatttt tgagatattc cttaaaaata cccacttaat
     1381 caatcacaaa acaaatatat aaacaagcaa tcattagatt agcttatttt taatgaaatt
     1441 attatatact tcaataagtt acaaaaaaac tctatattta aattattgcc ataatacacc
     1501 tgaagcgaat tgcacaggtt cacacaatct atttaacgct tgcagaatac ccctctttac
     1561 gatcgataaa aagtcctttt cttatttatt ttgaagcgta aaatgcgggg tgatgaagga
     1621 gtggtgattt tattttcacc gccattaggg cggcattgcc gatatttttt tgcggtacaa
     1681 atatgccgtg ttcatggcct gtacgagata acatgacgga tcacttttat gagcaaaaaa
     1741 tttgaaggca gcgtcgcgcc ccgcgagcgt attaatatat cgtatgtccc aaaaacagat
     1801 ggccagacgg ctgaagtgga acttccgctt aacatgctcg ttgtcggcga taccggaaat
     1861 acgcaggaaa catccccact ggatgaacgt caggcagttt ccgttaacaa gcacaatttc
     1921 ggtgcagtga tggctgaagc agccattggc ctcaatttta ctgtccccgc cacgctgaag
     1981 ggaagcacaa cagacgatga actgaatgtg gcgctgaata tcaaatcact ggatgatttt
     2041 tcaccagata gcgtggcccg gcaggttccg gaagtgaata aattgctgga actgcgtgaa
     2101 gcactgactg cactgaaggg gccgatgggt aaccttccgg cattccgtac ccagctccag
     2161 gcattgctgg aaaatgaaga gtcccgcgag cagttgctca aagaaatcgg tctggtgagc
     2221 aataaataac caccactatt tcattaaaca gggttatcca ggcatgacgg cacacaaaaa
     2281 tatctccggg acataccacc tctctgttgc agacattctg caggtggttt accaggtatg
     2341 tttcagcccg tccgttgaaa tcaaccagga tggtgtggct gcgctgatta caacactgga
     2401 tcgccgtatc agtgatctat tggatgagat tatccatttt tgtgagtttc agcagagcgc
     2461 gtcgcactgg cagcgcgtac ttcactgatg gcgaaaagcc ggtttcagaa acacagctaa
     2521 aacagaagga ttttcagtat gtctttacag gaagaggaac tcgtttccag tcatgccggg
     2581 cagcctgagc aggcatcgtc gctgcttgat caaatcatgg cacagacccg tattcagccc
     2641 ggttcggaag gttatgacgt tgccctccag ggtgtgacag cctttattgc cagcatcctg
     2701 caatccactg catctgccga accggtcaat aaactggccg tggacagcat gattgcagat
     2761 attgatgaac gcatcagccg ccagatggat gtcatcatcc atgcgcctgc ctttcagcag
     2821 gtcgagtcgt tctggcgctc actgaaaacc atggtggatc gcgttgattt ccgtgaaaac
     2881 atcaaggtca acgtgcttca tgtcaccaag caggaacttc tggaagactt tgagtttgcg
     2941 ccagaaatta ttcagtccgg attttataag cacgtttatt cgtccggttt cggacagttt
     3001 ggcggtaaac ccatcgctgc cgtgctgggc gcttatgagt tcaaaaatac cgcgcctgac
     3061 atgaaactgc tgcaatacgt cagtgccgtg ggcgcaatgg cgcacgcacc gttcctgtcc
     3121 tccgtttccc cggagtttat ggggctgaac tcgtggaccg aactgcctaa tatcaaagat
     3181 ctttatgcca tctttgaagg tccggcctac accaaatggc gcgccctgcg tgactcggaa
     3241 gattcgcgct atttagggct tacggcaccc cgcttcctgc ttcgccagcc ctattcacca
     3301 acagataacc ctgttaagaa ctttaactat tacgaagatg tcagccagaa ccacgaagat
     3361 tatctgtggg gtaatacggc ctggatgctg gcatgcaata tcgcagacag ttttgccaaa
     3421 taccgctgat gtccaaacat tatcggtccg caaagcggcg gcgcagtgaa agatctgccg
     3481 atgcatctgt tcgaaacgat ggggcagatt caggccaaaa tcccaaccga ggtgctggtc
     3541 accgatcgcc gcgaatttga actggctgaa gagggtttta tcacccttac catgcgtaaa
     3601 gactctgata atgcagcctt tttctctgca aactcggtac aaaaaccgaa gcactttccg
     3661 ggaaaagatg cagaaaccaa ctataagctg ggtacgcagc ttccttatct cttcatcatc
     3721 aatcggttag cgcactacat caaggtgttg cagcgtgaac aactggggtc atggaaagaa
     3781 aggagcgact tagaaagaga gcttaatacc tggatccgtc agtatgttgc cgaccaggaa
     3841 aacccgcctg ctgacgtgcg cagccgcaaa cctctgcgcg ctgccaaagt tgaggttatg
     3901 gatgtagaag gcgaacccgg ctggtaccag gttgcgctaa gcgtgagacc tcattttaaa
     3961 tttatggggg caaattttga gctttccctg gttggccggt tagacaggga gtaacacacc
     4021 atgataccgg atcgggaaag aaccacaggt tctctttttg agcgtatgga agcctcttcg
     4081 gccagaaacc ggcagggagg cagtattcat tcgctcaggc aatccattcg ccagaaccta
     4141 cgaaacattc ttaatacccg gtccggtagt tgccggggtg cgcctgagct gggcattgat
     4201 gaaccggaag gggctgaaaa cttccgcgaa tccatgagtc gggccatcga gcaatgtata
     4261 gaacgctatg aaccccggat ctcacacgct gaagtgcagg cagtggtttc atccgcctcc
     4321 agcccgctgg atatgacgtt tcatattaca gcctgggtga catttaacga aacgcatgag
     4381 gtgcttgaat ttgatatggc accgaatggc agccagcatt accgggtgga ctgacaaatg
     4441 gaatttgaag aacgctattt ccgggaagaa ctcgattacc tgcgccagct tagcaagctg
     4501 ctggcaacgg aaaaacccca tctggcccgc ttcctggccg aaaaagatgc agatccggat
     4561 attgaacgcc tgctggaagg ggtggctttt cttaccggca atctccgcca gaaaattgag
     4621 gatgaattcc cagaactgac gcacgggctt attaagatgc tatggcctaa ttacctgcgt
     4681 ccggttccgg caatgaccct tattgaatat acgccggata tggataagtc ttctgtaccg
     4741 gtgttaatcc cccgtaatga gcagtttaca accaacgccg gggaaatcag agttgatgaa
     4801 gtgctgccct ctgatgctaa aaaggaggag ccgcctccct gtaccttcac cctctgccgg
     4861 gatatctggc tgctgcccgt tcgcctggag cagattgaaa accgcagtac gacccgtaat
     4921 ggtgttatca acatcacctt ttcggtcgca ccgggaacgg acttccgcac gctggatctg
     4981 aacaaacttc gcttctggct cggcaatgac gacaactata cccgtgacca gctttattta
     5041 tggttctgcg aatacttgca gggtgccgac ctgactgtgg gtgaacagca tattcgcctg
     5101 cctgagttta tgctaaaagc tgtcggtttt gagccgcagg atgccatgct gccctggccg
     5161 aaaaacgtcc acagcggcta ccggatcctt caggagtatt tctgttaccc cgatgcgttt
     5221 ctcttttttg atctttgtgg ttgtccggct ttgcctgacg gattgcaggc ggaattcttt
     5281 accctgcaac tgcgtttttc gcgccctttg cccgtggaca tccggctgcg ccgcgattcc
     5341 ctgcgcctgt attgcgcacc tgccattaat ttatttatcc accatgcaga agccatcacg
     5401 ctggacaacc ggcgggcaga ctatccgctg gttcccagcc gccattaccc acaacattac
     5461 gatgtatttt ccgttaacag tgtggtgagc caggtccagg atatgttcag gaaaaaagat
     5521 ctggggcgtc ctgtttcgac gcaggccgcg cgccagtggc cagcctttga aagtttcagc
     5581 catcagatag aatacagccg gaagcgggaa gtggtgtact ggcatcaccg gaccaaaaca
     5641 tccctgttcc atcgcggctt tgatcatacc cttgccttta tacatgctga tggcagttat
     5701 ccgtcagacg aatctctgct cagtaatgaa gtggtttcgg tatcgctgac ctgtaccaac
     5761 cgtgagcttc cgtcacaaat tcgttccggc gatatcaccg gcacaaccgg aaaaaatgcg
     5821 gctgttgctt catttcgcaa cattacccgc ccgacgcaac cactctggcc ggtcattgat
     5881 ggcagcctgc actggcccct actctccgcc atgaacctga attatctgtc attactggat
     5941 acggacgcgc tgaagcaggt catcgccaac tttgatcgcc acgcaatcca tcatccgcag
     6001 acggcacggc tgtcacaaca aaagctggat gccattgagc gtctggagac ccgccccgtt
     6061 gatcgcctgt ttacgggtat tcccgtccgg gggctggctt ccacgctgta tctgcacccg
     6121 gagccgtttg tctgtgaagg ggaaatgtat ctgctcggta cggtgctttc gcattttctg
     6181 tcgctgtacg ccagcgttaa ctcattccac atgctgaccg ttgtgaacac agaaagccag
     6241 gagacatgga aatggacgga aagaatcggg cagcatcctc ttatctgagt ccgggaaatc
     6301 ccccggcaga taaagagcag aatgatccgc tggcacaggt tttccataat gcctgctctt
     6361 acaatttttt tgcgatggcg gagctgttgc accgcctggc aaagggtgaa aaggggacgc
     6421 cagaattatc cctgcgcgac gatccggcac aggaaaccct gcgtttcagc gccgatgcca
     6481 gccttgcctt cccgtgcagt gatatcagtg cgctgaaacg ggatacgtca ggggcattca
     6541 ggatgaccac cacctttatg gggttacagg ggagccagtc gcctctcccc ggttattacc
     6601 tcgctcacct ggcctggaaa gcggtacatg aacaaagtcc ggtaggtgat tttctggata
     6661 tgttcagcca ccgcctgacc cagtttgtct ggcatatctg gcgtaagtac cgctatcaca
     6721 tcagttttcg taacggcggt gtggatgcgt tctcgcagcg catgtactcc cttgtcgggc
     6781 tgggccaccg ccagcttcgc gataagctgg cgattaatca cagcaaaatg ctggcgtatt
     6841 ccgggatcct ggcaaatccg ggacgatccc cggaaattat ctgtggcctg gtgtcccact
     6901 gctttgattt gtcagaagtg acactgcaaa actggcaacg tcgcaaggtg gatattgaac
     6961 cggatcagca aaatagtctg gggagctatt ctcttaaaaa tggcgaaaaa ctggcgggaa
     7021 gatcggtact gggcaacttt gttctgggta cacgagtgcc cgatctcagc ggaaaattcc
     7081 agctcagtat taccagcctg acccgaaaac agttcctctc ttttctgccg tccggcgaaa
     7141 acttcctgcc actgacgatg ttcgtgtcct tcatcctgcg cgatcagctt gcctgggact
     7201 tacatctggg gctggcgccg gagcaggtgg gcgcaatgcg cctgggcgat aacaaaagtg
     7261 cgctgctggg ctggaccagc ttcctcggca caccggaaga acgaccctca gtcaccatca
     7321 gggtacggtc gtaaattatg ataatcacgg agcagaatat gccggaagag aaactacaga
     7381 cgctttcatt gcaggtcatt aacggcagtg agctggaaag cgggcgggcg gcgcgctgtc
     7441 tgttcacaca gcagggaaat gtcggccatg gccccgaatg ccactggtcg gtacaggatc
     7501 gtcagcagag cattccggcc caggctttta ccgttatcct gcacgatggc acattttgtc
     7561 tgcgcccgca gacggcacaa ctgtggctga atcaggcaaa agtcacagca acatcagacc
     7621 taatacagtt gcgccagggc gatgagatcc agatcggacg gctgatggtg agggttcatc
     7681 tgaaccgggg agatattccc cattacgatg aggaaatggc cactcccgaa accatcgtta
     7741 ccaatcgcga tatgctcacg gataccctgc tatcaacgga gggtgcgcca cactatccgg
     7801 gaatgactca ccggcaccag cttgcagaca ccgtggtaaa cggtttttct gccgatccac
     7861 tccaggctct tcagtccgaa agcctgatta ccacgggcga tccgctttca ggcattgcgg
     7921 ctgtccggcc atcggcaccg ctgtccgatc cggcaagtaa tggggggatc aatactccgt
     7981 ttatggatct gccgcccatt tatgccagcc ccggcgatcg taatgacgac gtttctgcgg
     8041 cagaaatggc acaacgccac gttgcggtca cccccttact gcgcggtctt ggcggctcgc
     8101 ttaccatgag caattccgac gatgcggatg attttctgga ggaggccgga cgaacgttac
     8161 aggccgcaat aaaaggtctg ctcgatttgc agcagcagcg taacagcctc tcagacaaac
     8221 atttgcgccc gctggaagat aacccgctgc gcctgaacat ggattacgcc accgcgctcg
     8281 acgtaatgtt tgccgaaggt aaaagcccgg tacatctggc ggctcccgcc gccgtcagtg
     8341 aaagcctgcg caatgtccgc caccacgaag aagctaaccg ggcagcgatt gtggagtcgc
     8401 ttcgtgtcct gctggatgct ttctcaccac aaaatctgct gcgccgcttt gtgcagtacc
     8461 gccgcagcca tgaactgcgc cagccgctgg atgatgccgg agcatggcaa atgtacagcc
     8521 attattacga agaactggcc tccgatcgcc agcaggggtt tgagatgctg tttaacgagg
     8581 tctacgccca ggtctatgac cgggtgcttc gtgaaaaaca gcgggagccg gaagcatgaa
     8641 caggaaggcg tttctggcct gcgtgctgat gtgcgtcata ttaaccggct gcgaaacggc
     8701 gaaaaaaatc agtgaggtga tcaaaaaccc ggatatccag gtcgggagcc tgaaggagca
     8761 accttcggaa ataaccgtca ccctgctgac cgaaccggac accaatacca atgcggaagg
     8821 tgaatcagcc gccgtggatg tgcaactggt gtacctgact gacgactcaa aattgctggc
     8881 agccgactat gaccagatag ccagcacgcc gctaccggat gtgctgggta aaaactatat
     8941 cgaccaccag gatttcaacc tgttgccgga caccatcaaa accctgccgc cggtaaagct
     9001 ggatgagaaa acgcagttta ttggtgtcgt ggcctatttc tccgatgacc aggccactga
     9061 atggaaacaa atcgagacgg tggaaggaat cggccaccat taccgcctgc tggtgcatgt
     9121 acgtcaaagc agcatcgaaa tgaaaaaaga ggatgaataa ttatggcaac cacccgcaac
     9181 aaggtgatgt ggcaggaagg gatgctgatg cgtccacacc atttccagca gcagcagcgt
     9241 tacaacgact acctggataa ccagcgtttc cgggccatga atgatttatc ctggggattt
     9301 accgaactca ccctcaacaa tgaactgctg gcgcagggta agatcatgat tgacagcgcg
     9361 tcaggcacac tgcccgacgg caccgtcttt tctatccccg accaggacgc actgcccgat
     9421 ccgctgcacc cgcaaaattt tccggacgaa agaagccgca atatctacct cgctctgccg
     9481 gtcgccagtg atgtgagaaa tgaaatcagc gacggacggc gaatcgggcg ttaccggctg
     9541 aattatgccg atgtccggga tttgcattca gaagaaggtg acgcgcgaac attgacgctg
     9601 ggtcaactga cgccgcgcat tatgagcggt gcagaagata tgagcgccta tattacgctc
     9661 ccgctttgcc gtatcagcga tcgccgccga tggctccctg acgttggatg acgattttat
     9721 cccctcctgc cagaatattc aggtcagtaa gaaactgcgt gtttatctca aagaggtaca
     9781 gggggccatt ggcggacggg caagcgatct ggcaaaccgt attggctctc cggcgcagag
     9841 cggcatcgcg gatgtggcgg aatttatgat gttgcagtta cttaaccgta accagacccg
     9901 gtttacccat cacgcccgtc gatcccagct ccacccggaa gatttctacc ttgatcttgc
     9961 cgggttgctg ggtgaactga tgacctttac cgagccgtcg cgcctgccct gcccgcttga
    10021 tgtgtatgat catcatgacc tgaccaaaat atttaaaacg ctgttaccgg aagtcaaacg
    10081 ggcgctgcat accgtactgt cgccaagagc cgtcaatctg ccgctgcatc tgcgtgacgg
    10141 tatctggcag gccgatatcc atgacacaga actgctgcaa tctgccacct ttgtgctggc
    10201 cgtggcggca aacatgccgg tcgatcagat ccagcgccag tttatccagc agtcgaaaat
    10261 ttcctcgccg gaaaaaatcc gcaatatggt cagtgtgcag atccccggta ttccattgcg
    10321 tgcccttatg gtggctcccc gccagcttcc ttaccattcc gggttcagct atttcgaact
    10381 cgacaagagt ggccaggcct ggacagaaat ggctgccgcc ggggccgttg cactgcatgt
    10441 ttccggcagt ttcccggatc tgaacatgca actgtgggcg ataagagggt aagacgatgg
    10501 atgaggggtc actgtctctg ccaccattta ccggacacga cgaaaaaagc cagcgcaact
    10561 accatctcgc gcttcgtggc aacagcctga atccgatgat cgacgcggcc acgccgctac
    10621 tcggtatggt aatgcgcctt tcaaccatga acagccagac gatgccggag catctttttg
    10681 ctcaggtggt gacagatgtt caggccgtgg agcaactgct tcaggagcag ggctacgaac
    10741 cgggcgtgat tatctcgttt cgctacatcc tctgcacgtt tattgatgaa gcggcgctcg
    10801 gtaacggctg gtcaaacaag aacgagtgga tcaagcagtc attgctggtc catttccaca
    10861 acgaagcctg gggtggcgag aaagttttca tcctgctgga gcgcctgatc cgtgaaccca
    10921 aacgttacca ggatctgctg gaatttctct ggatctgttt ttctctggga tttcgcgggc
    10981 gctacaaagt ggcagcacag gatcaggggg aatttgagca gatctaccgc cgtctgtacc
    11041 acgttctgca taaactgcgc ggcgatgccc ctttcccgct gttacaccag gacaaaaaaa
    11101 cccagggcgg gcgttatcag ctcatcagtc gcctgactgt taaacatatt ttttgtggtg
    11161 gcgttgtcgt gctggcgttg ttttacctgt tctatctgtt gcggctggac agccagacgc
    11221 aggacatcct gcaccagcta aacaaattac ttcgataagg acgtttatga tccagattga
    11281 tcttcccacg ctggtaaaac ggctgaacct gttctcccgc caggcgctgg agatggccgc
    11341 ctctgaatgt atgagtcagc aggcagcgga aattaccgtc agccatgtgc tcattcagat
    11401 gctcgccatg ccacgcagtg acctgcgggt tattacccgc cagggcgata ttggcatgga
    11461 agagttgcgc caggcgctga cggtagagaa ctacacaacc gcccgttctg cggacagcta
    11521 cccggcgttt tccccgatgc tggttgagtg gcttaaagag ggctagctgc tggcgtcggc
    11581 tgagatgcag cacagcgaac tgcgcggcgg cgtgttgctg ctggccctgc tgcattcgcc
    11641 gctgcgttat ataccgcctg ctgccgcccg gctgttgacc ggcattaacc gtgaccgtct
    11701 gcaacaggac tttgtgcagt ggacacagga gtcggcggaa tcagtcgtgc cggatgcaga
    11761 cggtaaaggc gcaggcacaa tgacggacgc ctctgacacc ctgcttgccc gctatgccaa
    11821 aaacatgacc gcagacgccc gtaacggcag gcttgacccg gtactgtgcc gcgaccacga
    11881 aatcgacctg atgatcgaca ttctctgccg ccgccgtaaa aacaacccag tggtggtggg
    11941 cgaagcgggc gtgggcaaaa gcgcactgat tgaagggctg gcgctgcgca tcgtggcagg
    12001 ccaggtgccg gacaagctga aaaacaccga tatcatgacc cttgacttgg gcgcattgca
    12061 ggccggggcg tcggtgaagg gtgaattcga aaaacgtttc aaagggctga tggcggaggt
    12121 catttcctcc ccggtgccgg tcattctgtt tatcgacgaa gcacataccc tgattggcgc
    12181 gggcaaccag cagggcgggc tggatatctc caacctgctc aaaccggcgc tggcgcgcgg
    12241 cgagctgaaa accatcgccg ccaccacctg gagcgagtac aaaaaatact tcgaaaaaga
    12301 tgccgccctg tcgcgccgct tccagttggt gaaggtcagc gaacccaacg ctgccgaagc
    12361 caccattatt ctgcgcggtc tgtcggcggt ctatgaacag tctcacggcg tgctgattga
    12421 tgatgacgcc ttgcaggccg ctgcgacatt aagcgagcgt tatctctccg ggcgtcagtt
    12481 accggacaaa gcgattgatg tgctggatac cgcctgcgcc cgtgtggcca tcaacctgtc
    12541 gtcgccgccg aagcaaatct cggcgctgac cactctgagc caccagcagg aggcggaaat
    12601 tcgccagctt gagcgcgagc ttcgcatcgg actgcgtacc gacacatcac ggatgaccga
    12661 ggtgctggtg cagtatgatg aaacgctgac ggcgctggat gaactggaag cggcctggca
    12721 ccagcagcag acgctggtcc gggagattat cgcgctgcgc cagcagttac tgggcgtggc
    12781 agaggacgat gcggcgccgt tgccggacgc agataccgtg gaggatacgc agccagagtc
    12841 agagtcagag tcagagtcag agtcagagca ggataatacc ggtgccgaac cggctgatga
    12901 ggccgacaga gaacaaccgg aagagaccgc tgaaacagtt tccccggtac agcggctggc
    12961 ccagctcact gccgaactgg acgccctgca taacgaccgg ttgctggtct ccccgcacgt
    13021 cgataaaaaa cagattgcgg cggtgattgc cgaatggacc ggcgtgccgc tcaaccgcct
    13081 gtcgcagaat gaaatgtcgg tcatcaccga cctgacgaaa tggctgggtg acaccatcaa
    13141 aggccaggac ctggcgattg ccagcctgca taagcatcta ctgaccgcac gcgccgacct
    13201 gcgtcgtccg ggacgcccgc tcggtgcgtt cctgctggct ggccccagcg gtgtgggtaa
    13261 aaccgaaacc gtcctgcaac tggcagagct gctctacggc ggtcgccagt acctgaccac
    13321 catcaatatg tccgaattcc aggagaaaca caccgtctcg cggctgattg gttcgcctcc
    13381 gggctacgtt ggctacggtg aaggcggcgt actgaccgaa gcgattcgcc agaaacccta
    13441 ctcggtagta ctgctcgatg aagtggaaaa agcgcacccg gatgtgctca acctgttcta
    13501 ccaggcgttc gataagggcg aaatggcaga cggtgaaggc cgcctgattg actgcaaaaa
    13561 tatcgtcttc ttcctgacgt ccaacctcgg ctaccaggta atagtcgagc atgccgatga
    13621 cccggaaacc atgcaggaag tacagtatcc ggtgctggcc gacttcttca aacctgccct
    13681 gctggcgcgt atggaagtgg tgccgtatct gccgctgtcg aaagagacgc tcgccaccat
    13741 tatcgccgga aaactggccc gtctggataa cgtgctgcgc agtcgctttg gtgtagaagt
    13801 ggtcattgaa ccggaagtga cggacgaaat catgagccgc gtcacccgcg cggaaaacgg
    13861 cgcgaggatg ctggaatcgg tcatcgatgg cgacatgcta ccgccgctct cgctgctgct
    13921 gttgcagaaa atggcggcta acacggcgat tgcccggatt cggttgtcgg cagtggacgg
    13981 cgcatttacg gcagacgtgg aagatgctca gaacgacgag tccgtcacaa aggatgaaac
    14041 ggttttatga gcaggttgcg cgttttccgg cagacaggat ggtttatcgc gggcctgatg
    14101 acgggcttac ccgccactgc cgcgcccgca gaagcatcgt caatggccgg tgtggcggtc
    14161 gccgtcgcca ccacgacccc gccggatgcg acagccacgt tacaggccat gcagtcctgt
    14221 cggcgggagt ccgcggcgct ggagcggctt gactgctatg accgcctgct ggcaccgctg
    14281 tccccgtcag ggtttgacgg ggcgctggtc aaagccggtt ttgtgggcga ggcctggacc
    14341 cgcgccacag agcaggaaaa acgccgcgag ggcaacacca cagaactgct ggtgacgcag
    14401 gtgccggggg agcgcccgac ggtggtgata accaccccgg ctatcggtca tgttccgcca
    14461 cgcccggtgc tgatgttcag ttgcgtggac aatatcaccc ggatgcaggt ggcgctgatg
    14521 caccctctgg atgtccatga tatcgccgtc actctgaatg ccgacaaccg ggcattgcgc
    14581 agccactggt ttgtgcgcga gaacggcacc ctgctggagt ccagccgggg gttatccggg
    14641 attgatgaaa ttaagcaact gttcggcgca aaaacgctga cggtggatac cggcgcagac
    14701 aatgccgccg gaaagctgac ctttaacatt gacgggctgg caagggcgat tgctcccctg
    14761 cgcgatgcct gccactgggc gggagaataa aaatatggca atggacttac gcgatccgaa
    14821 tgtctggata tcgcacctgc tggaaaacct gccggaagag aaactggcat cggcgctgaa
    14881 agatgacaac ccgaactggg agtatatcga cggcgaaatc gtcaagctgg gatcgctggc
    14941 ccacagtcaa ctggatatcc cggaactcca gcgccggggg ctggtcattc tggcttcgga
    15001 gagtaaggat tttcgtctgc tggcgcatct gctaagaacc ctgcaacacg ccggtgatcc
    15061 tcatctcgca ttgcgcctgc tggcgctgta tgtggagcat tactgggcgg tggctgcgcc
    15121 gcagaatatg gcgcataaaa aacgctttgc cagccaggtc attaaacgct ttgaaacagg
    15181 tattgaagtt ttttcacaga acgccgccac agcgcagcgc gatgcgctgt caggggaact
    15241 ggcaaaactg gcgcaatgct ggcagtcaca taacgccccg gaactggcac aggctactga
    15301 tgatcttttt gccctgtacc agcgtgcgtt taatcgtgcg gcaccggctc ctgtccccac
    15361 tccggcggcc tccggtagtt caccacagac caccgccacg tctgaaagcg gcgtgacgca
    15421 acccagtgct ccggctcccc aaatcgtcat cgacagtcac gacgacaaag cctggcgcga
    15481 cacgctgtta aaagtggcgg ccattttatg tgaacgccag ccggattcac cgcagggcta
    15541 ccgtctgcgc cgccatgcgc tttggcagaa catcaccagt acgccccagg cggaaagcga
    15601 tagccgtact ccactggctg cggtctccgc cgatatggtg gcagattacc atgctcagct
    15661 cggtagtgcc gatatggcgc tgtggcagca ggtggagaaa agcgtactgc tggctcctta
    15721 ctggctggat ggtcactgcc tttctgcaca gacggcgctg cgtctgggtt acaaacaggt
    15781 ggcagacgcc atccacgatg aggtcatccg cttccttgag cgtctgcccc agcttaccgg
    15841 gctgctgttt aatgaccaca caccgtttat cagtgagcag acgaaacaat ggctggcagc
    15901 ttcgcccgac gccaaagttg caccggttgc gcaaatcggt gaggaatcga aggcagccag
    15961 agcctgtttt gctgagcagg gtctggaggc ggcgctgcga tatctggaca tgctacccga
    16021 aggcgatccc cgcgatcagt ttcaccgcca gtaccttgcc gcacagttga cggaagaggc
    16081 ggggctggta cagcttgcgc agcagcagta ccggatgttg ttcaggatgg gattgcagat
    16141 gatggtggca gactgggagc cgtccctgct tgagcagcta gaacaaaaat tcacggcaga
    16201 acaataaaag agaacaggag tttttgtgtt cagattacca acaccccgac tactcagcgg
    16261 actcaaatca gccctgcgac cggcgatgcc ccggtttaaa gtctctgctt tctggctgct
    16321 gatactggcg tggatttttc tgctggtgtg gatctggtgg aaaggcccga tgtggacgct
    16381 gtatgaagaa cagtggctca aaccactggc gaaccgctgg ctggcaacgg cggcgtgggg
    16441 gattattgcc ctggtgtggc ttaccgtccg ggtgatgaag cgcctgcaac agcttgaaaa
    16501 aatgcaaaag caacagcgcg aggaagccgt tgatccgctc agcgtggaac tgaacgccca
    16561 gcagcgttat cttgaccgct ggctgctgcg cctgcaacgc catctcgaca accgccgttt
    16621 cctgtggcag ttgccgtggt atatggtcat cggcccggcg ggcagtggca aaacgacact
    16681 gctgcgcgag gggtttccgt ccgacattat ttatgccccg gagggcgcac gcggcgcaga
    16741 acaacgcctg tacctcacgc cccatgtcgg taaacaggcg gtgatctttg atatcgacgg
    16801 cacactctgc gctcccgctg atgcggatat cctgcatcgc cgtctgtggg aacatgccct
    16861 cggctggctg aaagaaaagc gcgcgcgcca gccgctgaac gggattattc tgacactcga
    16921 tttacccgat ctgctgaccg cagacaaacg ccgccgcgag catctgttac agacgctgcg
    16981 cagccgctta caggatatac gccagcatct tcactgccag ttaccggttt acgtggtact
    17041 gacccggctt gatttattac agggttttgc cgccctgttc cagtcactca acagacagga
    17101 tcgcgatgcg attctgggcg tcacgttcac ccgccgtgcc catgaaaatg atgactggcg
    17161 aacagagttg aatgctttct ggcagacatg ggtggatcga atgaatctgg cgttgccgga
    17221 tctgatggtc gctcagactc acacccgcgc gtctttattc agtttttccc gccagatgca
    17281 gggaagccgt gaaccgctgg tgtcactgct tgagggtctg cttgatggcg aaaatatgaa
    17341 cgtgatgctg cgtggtgtct atctcacctc ttcgcttcag cgtggacaga tggatgatat
    17401 attcacccag tctgccgccc gccagtaccg gctgggcaat aacccactgg cgtcctggcc
    17461 cctggtggac accgcgcctt atttcacccg cagcctgttc ccgcaggcat tactcgcaga
    17521 gcctaatctg gcaacagaga gccgcgcctg gctgatacgt tcccgtcgcc gcctgacggt
    17581 tttctctgcc acaggcggcg tggcagcact gctgctcatc accggctggc atcactatta
    17641 caacggtaac tatcagtccg gcatcaccgt gcttaagcag gccaaagcct ttatggacgt
    17701 gccgcctccg cagggggaag atgactttgg caacctgcaa ctgccgctcc tgaacccggt
    17761 acgcgatgcc acactggcct atggcgactg gggcgaccgc agccgtctgg ccgatatggg
    17821 actgtaccag ggacgacgta tcgggcctta tg
//
LOCUS       DAZPAC010000071        17764 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZPAC010000071 DAZPAC010000000
VERSION     DAZPAC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17764
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.071"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            140..1825
                     /locus_tag="ACHUPM_002031"
     CDS             140..1825
                     /locus_tag="ACHUPM_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFR0869812.1"
                     /translation="MNTALSIMGDASSNTAIDYRQEMNVIHEIVAECEKEISFMYQVH
                     DFVYGDERHNMINRLLRLNHRPDEDRSRLNRVWLDNVDLEWVKQNIWAEYWRKVTDMT
                     NVLLIMPASRRDEWRELFIEGKQEVIKTDRTGYQMKVKEFVGVPEFKAETVIPTMLNL
                     LNDRHKYLSERVYGLFKALSPAHKTNKTNGFSERLIIADCISDFWRDSVSVNYRKEDY
                     IDDLRVLLHFFAHKEFITINRTAEVLSAAYRANDCQTGDWMNVDGNLMRVKMFKNGNV
                     HFEIHPDVAWKLNEVLAYSMPAAIPAPCRTAPKTRAPKQFGLIQKTISVPVRTALRDG
                     RLSKDKGVWYFSDSALQKSQVEELERTLSFIGGVQEKKHWQFPYEIGHTLNTIVATGL
                     IPDTKSHQFYPTPRLIAEYVARATELKPGEKLLEPQAGRGDLLAYINADLEDVTCIEI
                     APLFADILRGKGYTNTICCDFIKWSEDNAGYQFDKIVMNPPYSLGRHREHTLAALGHL
                     KVGGRLVAVLPGTAPILDWMTMDNYVYARGKSFTNEFEDTGITVSVYVFKRVK"
     gene            1887..2591
                     /locus_tag="ACHUPM_002032"
     CDS             1887..2591
                     /locus_tag="ACHUPM_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869813.1"
                     /translation="MNNLQLEHFNVTGHSDFPFKFTLKGYAEDAVGQIIIDKGIVKFE
                     GDFDESAKTFIDFIAKRWSEQWKDLEKRANEFDRFMDTMDTAKEALAAGTPLDLESLF
                     NSTMASEMFATMFAGEFVRSGAKNYLELDYNVPAIGDFVVTIQREEGKTPGERVAELE
                     AVVDQRNGECDRLINELHALREERICAGSNTRNAADIYFQLVEECQIPPGGSLVDYVR
                     HLMAEVNSSHKDGEVR"
     gene            2591..2971
                     /locus_tag="ACHUPM_002033"
     CDS             2591..2971
                     /locus_tag="ACHUPM_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869814.1"
                     /translation="MFGIDAQRIAAFAKSPLDNPLSRSEQMELARLFLHIQKQADIFN
                     NMSNQPILDGHIQMVINSHEKGWAAIVPCTITYRLAKEVQEFRKASVESESTKAAINT
                     LIRMGFTWTGGAYWQAPHPILYGN"
     gene            3110..3652
                     /locus_tag="ACHUPM_002034"
     CDS             3110..3652
                     /locus_tag="ACHUPM_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869815.1"
                     /translation="MSNKIENPVVLIHKRENHDSYEVAITNGSHDYYDGLLMASVSPD
                     EADNSFAVFAMVGYYMAAEIEKLRAQRDALAAENAALKESERAFDAMCAEEHGDNWVS
                     ELTETPATDAFLAEVRAQGVDAAIEAAKNLVAQEYEYKDFKAAQSDCCMHPGSDLVGK
                     VEMTEWLVDFAAQLRKGGNQ"
     gene            3649..3903
                     /locus_tag="ACHUPM_002035"
     CDS             3649..3903
                     /locus_tag="ACHUPM_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869816.1"
                     /translation="MSNSARLQLGFSPLSKTIMLAKMRDVEGGRMRVGNDPGRDVTNE
                     AAQLVWQLVMAEGGEIVWELDDGSRMVLKAEKQEAASERN"
     gene            3890..4420
                     /locus_tag="ACHUPM_002036"
     CDS             3890..4420
                     /locus_tag="ACHUPM_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFR0869817.1"
                     /translation="MSEINYQALREVAERAIPAMERLLMLPADDDLLSEQELKDSGVD
                     IDALNAFKFLAGPETVLALLDEINALEETRINDVCRIAELTKQLELAKSKLNEQREYY
                     EGVISDGSKRIAELESDSQAQKLVEAIIVAIENEQERLFDEDYLMDSKECIDVIREEV
                     KRWNDSRAAGIRIKGE"
     gene            4422..4745
                     /locus_tag="ACHUPM_002037"
     CDS             4422..4745
                     /locus_tag="ACHUPM_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HFR0869818.1"
                     /translation="MTTSHSAITQKKAFHILERLEALATEEEISPEKLVEFSRVILRR
                     KNDMERLTSGAPSLSVRRTLCCSFCNKSQYAVKKLIAGDAVFICDECVDVCNRIIRGE
                     KEGSA"
     gene            4742..5263
                     /locus_tag="ACHUPM_002038"
     CDS             4742..5263
                     /locus_tag="ACHUPM_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869819.1"
                     /translation="MKFSKFSELVNRILSNNHSHRRDMDVTIVVHSPGRIGSTPSVEV
                     QSIQAGFDWDAGKVMIFPAQPLTTLTPEQVADITDSVRKGQSWHAYQEYKKHKEQLEK
                     LSMELEAAKQREKDLFMENVRLKSGIAGLIHLGIRYADVEVMKIAGDAQLSTPCTDSI
                     INSIAAGIRIKGE"
     gene            5265..5528
                     /locus_tag="ACHUPM_002039"
     CDS             5265..5528
                     /locus_tag="ACHUPM_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869820.1"
                     /translation="MATLTKKERAWLNELQDVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDERILFPSSVESTAG"
     gene            5539..5925
                     /locus_tag="ACHUPM_002040"
     CDS             5539..5925
                     /locus_tag="ACHUPM_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869821.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AKPIGAFHIAEQQVDGTSDYLKDGEWPIDNGIIEVYAAPPVPVVPEEKPMPNPLSMYA
                     VDAVAAIAEVRGWNACRAAMLQGKGE"
     gene            5927..6502
                     /locus_tag="ACHUPM_002041"
     CDS             5927..6502
                     /locus_tag="ACHUPM_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0869822.1"
                     /translation="MTTITDKKQYPGEQYLNELITNIEFAARAPVEVVRAMAAELQKR
                     READSAEPASNHEELPLDYLQGQKDGLEWAAQLAEANHPQTGDWLYDDPLDLAKAIRK
                     GPDIPEFAGPHPVTPDGWISCSERMPEKGQNVLISVNFDSSLVEPLICSARYTGITFR
                     RGDVTIKPGNGIEQATHWMPLPEPPQESKSE"
     gene            6495..6716
                     /locus_tag="ACHUPM_002042"
     CDS             6495..6716
                     /locus_tag="ACHUPM_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869823.1"
                     /translation="MNKAELFQKISALATECHAIASELDVGDERTEMFEIYSVLRNLC
                     RRGYATQVGRMTNPLLSSCDEDDSDEDDE"
     gene            6713..7567
                     /locus_tag="ACHUPM_002043"
     CDS             6713..7567
                     /locus_tag="ACHUPM_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869824.1"
                     /translation="MMHKSVAGEFQKEVDNTTVLLDDILSILALLETGDWSEHCTKTE
                     LGGRLEREITRLIGDAQEATVTSYELIAEAWRLMDGQDPKTSDWHSKASKYLNSNRVE
                     KVDDVKPKPVDHGFRDNCECSSCQTTARICSELTDKSSLIYEVNVGGNTWGECTRAAY
                     VRAKDNGELTRVVTNHPNNELKDHQIRELVNELRDIAVTYHGTQQLREKIARAVNNSL
                     KGGQSQPKRIRITHIGENGTEIPQFSVGDIFDVHGEEFGEPHITMPSGHVYWLRAMEK
                     YGDKWEVL"
     gene            7581..8105
                     /locus_tag="ACHUPM_002044"
     CDS             7581..8105
                     /locus_tag="ACHUPM_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFR0869825.1"
                     /translation="MTKFGKLEAHLLTRNYRYDVHPYRQWIDESEGVVTFPLYRFDGM
                     LAGYQTYRPGAPKQHSNPKMARYFTRAHGRQLLWGTYLPLKDGPIWITESIFKSAAVH
                     NAGGNSWALLGSTFSAGLRRQLAMLPYDFRVIGDNDAAGESLVKSFGKGFVAPDLDEL
                     QPNEVSHLIFSHSQ"
     gene            8278..8667
                     /locus_tag="ACHUPM_002045"
     CDS             8278..8667
                     /locus_tag="ACHUPM_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFR0869826.1"
                     /translation="MGFPSPAADYAESRISLDQQIIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCALDGEYRVKRYRKSPRQHLEDLRTGKKEALPKDDDGCT
                     GSNAVFGVITHIINDARSGEFDDCPVM"
     gene            complement(8705..9805)
                     /gene="apbC"
                     /locus_tag="ACHUPM_002046"
     CDS             complement(8705..9805)
                     /gene="apbC"
                     /locus_tag="ACHUPM_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR0869827.1"
                     /translation="MSESKSPDALRAMVAGTLANFQHPTLKHNLTTLKALHHVALMDD
                     TLHVELIMPFVWNKPFEDLKEQCSGDLLRITGAKAIDWKLSYNIATLKRVKNQPGING
                     VKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQRPT
                     SPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDYLVL
                     DMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVENMS
                     VHICSNCGHHEPIFGTGGAQKLAEKYNTQLLGQMPLHISLREDLDNGTPTVISRPDSE
                     FTAIYRDLADRVAAQMYWQGEVIPGEIAFRAV"
     gene            9963..11996
                     /gene="metG"
                     /locus_tag="ACHUPM_002047"
     CDS             9963..11996
                     /gene="metG"
                     /locus_tag="ACHUPM_002047"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR0869828.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKCMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLTSELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATAAPVTGPLADEP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            12158..12457
                     /locus_tag="ACHUPM_002048"
     CDS             12158..12457
                     /locus_tag="ACHUPM_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869829.1"
                     /translation="MLNITQAYQVALISSSHTSEEDARRFMIASNELDWVSQIEYGCI
                     VHAGLQDDDWKDELRRYGISEGAIANIQKVLDVGFNSVHFDCGAPVVEGLECWQW"
     gene            12494..12679
                     /locus_tag="ACHUPM_002049"
     CDS             12494..12679
                     /locus_tag="ACHUPM_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869830.1"
                     /translation="MLKEDSYLLKALEMSGVEPTVKNAHDIYLQFSTELKQLINKYGV
                     NDSSVKAMIDDELDHLI"
     gene            14224..14439
                     /locus_tag="ACHUPM_002050"
     CDS             14224..14439
                     /locus_tag="ACHUPM_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869831.1"
                     /translation="MKMPVTVEVWGVDSLAECLDAVGPELYRKLWSFVPAEGESPKGK
                     DIWHLLSEDEKRELVGAVHSEFPDDQD"
     gene            14578..14907
                     /locus_tag="ACHUPM_002051"
     CDS             14578..14907
                     /locus_tag="ACHUPM_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869832.1"
                     /translation="MFANIDINQIKKLTQKEFDQFYELEGWSSTLINSRWVLELMTRD
                     DAPALMICDMGEDADFMDMSEFCVDTYNRSQKYYFTCDSENDVISKVYLHLVQHWDVQ
                     DFLAIFA"
     gene            15062..15373
                     /locus_tag="ACHUPM_002052"
     CDS             15062..15373
                     /locus_tag="ACHUPM_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869833.1"
                     /translation="MKKSLVLGLDKDQKRKEKPALVAQLTLLDIVANGTSIRLFRETA
                     VSFDKNTFTRYVMNVRRQRGKGWMAFQRMWPEHQLELALMEVNRVAQQEIQRASVMAI
                     A"
     gene            15499..15894
                     /locus_tag="ACHUPM_002053"
     CDS             15499..15894
                     /locus_tag="ACHUPM_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869834.1"
                     /translation="MSVKRENLTVDVYYASESAEGKNVAKITVVTYNTETGTEVQAST
                     IVRKGDTSGGEYEAQYQSIFDATDPLLLKIESYFRKVNKEVFETMMNMVNTVFASSLN
                     TNTTWIGQYGLRITSGIPADTLIPESVFA"
     gene            16013..16387
                     /locus_tag="ACHUPM_002054"
     CDS             16013..16387
                     /locus_tag="ACHUPM_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869835.1"
                     /translation="MGLDIYIETQPKNDLNNDASRKKVAYFRKFNALFGWMERNVGEV
                     KNCELLELTMNDICALKAHLMHMNESNCEEYLPTCEGFFFGSQEYDEGYWHDVGELKK
                     LVEELIKNHDFHNNRLTFCAWW"
     gene            16738..17220
                     /locus_tag="ACHUPM_002055"
     CDS             16738..17220
                     /locus_tag="ACHUPM_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869836.1"
                     /translation="MNFIATVNAPAHGNIAVTFSDIEKRVLGAWRDNETVELSAQEKC
                     IIARDIIGNRRYSRVFEKAYVVNSGFGTFVFPVRSGRFCQSKLIEFATQISVWIKTQS
                     SFKFSDDEAVSQGMRIANNAIKCKNITYTAGVDTWKLFCANFMLNVYASNRIHILDGV
                     "
     gene            complement(17303..17455)
                     /locus_tag="ACHUPM_002056"
     CDS             complement(17303..17455)
                     /locus_tag="ACHUPM_002056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869837.1"
                     /translation="MSQATKVIIIFPAHLSDNRWLDSTSFPMFFLHKGECSRFPKKSW
                     PFPVGS"
BASE COUNT         5028 a         3984 c         4366 g         4386 t
ORIGIN      
        1 aaagtcctgg ttataaatac atccaagtat gattagtcaa tgcctttatt ttaagtattg
       61 cctgttaatt tctatttagc acctttttat ttttgaaata attgcatcat taaacaaatc
      121 atttcttaat ggtgcaatta tgaatacagc cctttccatc atgggcgatg ccagctcaaa
      181 cactgctatc gactatcgac aggaaatgaa cgtcatccac gaaattgtgg ccgagtgcga
      241 gaaagagatc tccttcatgt atcaggtaca cgacttcgtt tatggcgatg aacgccacaa
      301 catgattaat cgcctgcttc gactaaacca ccgaccagat gaagatcgct cacgtttgaa
      361 tcgagtctgg ctggataacg tcgatctgga atgggtgaaa cagaatattt gggccgagta
      421 ctggaggaag gttacggata tgactaacgt tctgctgatc atgccagctt cccgtcgcga
      481 cgagtggcgt gagctattta tcgagggcaa gcaggaagtc atcaaaactg acagaaccgg
      541 ctatcagatg aaggttaaag agttcgttgg tgtaccggag ttcaaagcag aaacggtcat
      601 accaacgatg cttaatttgc tgaatgacag gcacaaatat ctctctgagc gcgtgtatgg
      661 cttgtttaag gcgctgagtc ctgcgcacaa gacaaataag acaaacggtt tcagcgagcg
      721 tctgataatc gctgactgca tttctgattt ctggcgtgac agcgttagcg tgaactatcg
      781 caaagaggac tacatcgacg atctgcgtgt cttgcttcat ttcttcgcgc acaaagaatt
      841 tattaccatc aaccgcactg ctgaggtgct atcagctgcg tatcgggcaa acgactgcca
      901 gaccggtgac tggatgaacg tcgatggaaa tctgatgcgc gtgaagatgt tcaagaacgg
      961 caacgttcac tttgaaatac atcctgacgt ggcctggaag ttgaatgagg tgctggctta
     1021 cagtatgcct gctgcaatcc ccgcgccatg ccgaactgcg ccaaaaacac gggcaccaaa
     1081 gcagttcggg ttaatccaga agacgatctc cgtgccggtt cgcactgcgc ttcgtgacgg
     1141 gcgattgagc aaagacaaag gcgtatggta cttctctgat tcagctctcc agaagtcgca
     1201 ggtggaagag cttgagcgca cactgagctt cattggcggc gtgcaggaga aaaagcactg
     1261 gcagttcccg tatgagatcg gccatacgct aaatacgatt gtggctaccg gtttaatacc
     1321 ggatacaaaa tcacaccagt tctaccctac cccacgcttg attgctgagt acgttgccag
     1381 agccactgaa ttgaagcctg gtgagaagct gttggagcct caagccggac gtggggatct
     1441 tctggcctat attaacgccg atctggaaga tgttacctgc atagaaatcg cacctctctt
     1501 cgctgatatc ctgcgtggaa aagggtatac gaacacgatt tgctgcgact tcataaagtg
     1561 gtctgaggac aacgcaggtt atcagttcga caaaatcgtt atgaacccgc cgtactcgct
     1621 tggtcgtcat agagagcaca cgctggctgc gctggggcat ctgaaagtcg gcgggcgtct
     1681 tgtagcagta ttgccgggca ctgcgccaat actggactgg atgacgatgg ataattacgt
     1741 ttatgccaga gggaagtcgt ttaccaacga gtttgaagac acagggatca cagtcagcgt
     1801 atacgttttc aaacgcgtta aatgataggt aaatacttac ttaatttgtg taagaatgta
     1861 ataactaaac gataagagaa aaacatatga acaacctcca gttagagcat tttaacgtca
     1921 caggccattc tgattttcct ttcaagttta cattgaaagg ttatgcagag gatgcggtag
     1981 gccagatcat tattgataaa ggcatcgtta agtttgaggg ggattttgat gaatccgcga
     2041 aaacattcat agacttcatt gccaaacgtt ggagcgagca atggaaagac ctggaaaagc
     2101 gcgctaatga gtttgatcgg ttcatggata caatggatac agcaaaagag gctctggctg
     2161 ccgggactcc gttagatctg gagtcacttt tcaacagcac aatggcctct gagatgttcg
     2221 ccaccatgtt cgcgggtgag ttcgtccgca gcggtgccaa aaactacctt gagctggatt
     2281 acaacgtccc tgcaattggc gatttcgtcg ttaccatcca acgcgaagaa ggtaagacgc
     2341 caggtgaacg cgtcgcagaa cttgaggctg ttgtggatca gcgtaacgga gagtgtgacc
     2401 gtttgatcaa cgagcttcat gcacttcggg aagaaagaat atgcgcgggt agcaatacac
     2461 gtaatgctgc ggatatctac ttccagttag ttgaggaatg ccagattcca ccaggtggct
     2521 ctcttgtcga ttacgtcaga catttaatgg cggaagttaa ctccagccat aaagatggtg
     2581 aggtgcgctg atgtttggca ttgacgcaca gcgtatagcc gcttttgcaa aaagccctct
     2641 tgataatccc ttgtctcgta gtgagcaaat ggagctggca aggctttttc ttcacattca
     2701 aaaacaggca gacattttca ataacatgtc taatcaacct attctggatg gtcacatcca
     2761 gatggtcatt aacagtcatg agaaaggctg ggctgcaatc gtcccctgca caattacata
     2821 caggttggcg aaagaggttc aagagtttcg taaagccagt gtcgaatcgg agtctaccaa
     2881 agccgcaata aacactctta ttcgcatggg gttcacatgg accggtggag cctactggca
     2941 agcacctcac cccattttat acggcaatta gacgctacaa atccttcggc atgaataaat
     3001 aaaggccata agttgtggcc ttaaataaat tgttttctac attttcttat ttgtgaaaat
     3061 aaaccagcac ttggtagtgc ttatgtaaca gcaaagaaga gaaaaacaca tgagtaacaa
     3121 aatcgaaaac cccgtagttc tcattcacaa gcgtgagaac cacgactcct atgaggtggc
     3181 gatcaccaat gggagtcacg attattacga tggtctgcta atggcctcag tgtcgcctga
     3241 tgaggcagac aactcttttg ccgtcttcgc tatggttggt tactacatgg ctgccgaaat
     3301 tgagaagttg cgggcgcaga gagacgcatt agcggcggag aatgcagccc tgaaagaatc
     3361 tgagcgagca ttcgatgcga tgtgtgccga ggaacacgga gataattggg ttagcgaatt
     3421 aacggagact ccagccaccg atgcttttct ggctgaagta cgtgcgcagg gggtggatgc
     3481 tgctatagaa gctgcaaaaa atctggtggc ccaagaatat gagtataagg atttcaaagc
     3541 ggcgcagagt gattgctgta tgcaccctgg ttcagacctg gtagggaagg ttgaaatgac
     3601 tgagtggtta gttgactttg ctgcccagct tcgcaaagga ggcaaccagt gagtaattca
     3661 gcacgactac agcttggttt ttcaccgcta tcaaaaacta tcatgctggc aaaaatgcgc
     3721 gatgttgaag gtggacgtat gcgcgttggc aatgatccag gtcgtgatgt taccaatgag
     3781 gctgctcaat tggtgtggca actggtcatg gctgaaggtg gtgagatagt gtgggagctg
     3841 gatgatggtt ctcgcatggt gttgaaggca gaaaagcagg aggcagccag tgagcgaaat
     3901 taattaccag gcactgcgtg aggtggcgga acgtgcaatt ccagcaatgg aacgcctgtt
     3961 aatgttgcca gctgatgatg acttgttaag tgaacaggaa cttaaagata gcggtgttga
     4021 tattgatgcc ctcaacgcct tcaaatttct ggccggacca gaaaccgtgc tggcactact
     4081 ggatgagatc aacgcattag aggaaacgcg tatcaacgat gtttgccgta ttgcggaact
     4141 aacaaaacaa ctggagttgg caaaatcaaa actcaacgag cagcgtgagt attacgaggg
     4201 cgttatctcg gatggaagta agcgcatagc agagttagaa agtgattctc aggcacaaaa
     4261 gttagttgaa gcaatcattg ttgcgataga aaacgaacag gaacgtcttt ttgatgaaga
     4321 ttacctaatg gattcgaaag aatgcattga cgtaattcgt gaagaagtaa agcgatggaa
     4381 tgattcccgc gccgctggca ttcgcatcaa aggagagtga gatgaccaca tcgcattctg
     4441 caattaccca gaaaaaagcc ttccacatac tcgaacgatt agaggcgctt gctacggagg
     4501 aggagatatc cccggagaaa ctggttgagt tcagccgtgt gatattgcgt cgcaagaacg
     4561 atatggagcg gctgacatct ggcgctccat ccttatcagt caggcgaaca ctttgttgca
     4621 gcttctgcaa caaatcccag tacgccgtca aaaagttaat tgctggggac gccgttttca
     4681 tctgcgacga gtgtgtggat gtgtgcaaca gaattatccg gggagagaaa gagggatcag
     4741 catgaaattt tccaaatttt ctgagttggt gaatcgtatt ttgtccaaca accacagcca
     4801 tcgtcgcgat atggatgtaa cgatcgttgt tcattcgcct ggtcgcatcg gttcaacacc
     4861 atcagttgag gttcagtcaa ttcaggcggg ttttgattgg gatgccggga aagtgatgat
     4921 ttttccagca cagccactga ccacgctaac accggagcag gttgctgata tcactgatag
     4981 tgtgcgcaaa ggtcagtctt ggcacgcgta tcaggaatac aagaagcata aagagcagtt
     5041 ggaaaaattg tcgatggagt tggaagccgc taaacagcgg gaaaaagatc tgtttatgga
     5101 aaatgttcga cttaagtcag gtatagccgg tctgatacac ctcggtattc gatatgcgga
     5161 tgttgaggtc atgaaaattg ctggagatgc ccagctttct actccatgca ctgacagcat
     5221 cataaacagc attgcagcag gcattcgcat caaaggagag tgatatggca actttaacaa
     5281 aaaaagaacg ggcatggttg aacgaattac aggacgttct tgatcgctgc ccatcaccga
     5341 aaaaaattgg tttttacacc attggcgata aaagcattta cctgtatgac ctgcgccgca
     5401 tggatgaaat catggaggct cttgataatc gttcgtcgat ggattggtgt gttgctgtcc
     5461 atgatatgaa tgccggattt gatgaaagga ttttgttccc ctcatcagtt gaaagcactg
     5521 cgggttaagg agtaacacat gaccactatt accaaagagc gactactgac aatcaaacag
     5581 tggcgcgaaa catacggacc tggtagcaac gttgtactgc cagcagaaga agcggaagaa
     5641 ctggcacgaa ttgctctggc atcgctggaa gcaaaaccaa taggtgcatt ccacattgca
     5701 gaacagcaag ttgacggcac aagtgactac ctcaaggatg gagaatggcc tattgataat
     5761 ggaattattg aggtctacgc cgctccaccc gttccagtag taccggaaga aaaaccaatg
     5821 cctaatcctc ttagcatgta cgcggttgat gctgttgccg ctattgcaga ggtgagaggc
     5881 tggaatgcct gccgcgctgc tatgcttcag ggaaaaggag agtgatatga ccactataac
     5941 cgataagaaa cagtatccag gcgagcaata tcttaatgag ctgatcacca acatagagtt
     6001 tgctgcaagg gcaccagttg aagtcgtgag agcgatggca gcagagctac agaagcggcg
     6061 cgaagctgat agtgcagaac ctgcaagtaa tcatgaagag ttgccgcttg attatctcca
     6121 aggtcaaaaa gatggtcttg aatgggctgc gcagcttgca gaagcaaatc acccacaaac
     6181 tggcgactgg ctttacgatg acccgctgga tctggctaaa gctatcagaa aaggtcctga
     6241 cattcccgag ttcgctggac cccatccggt aactccggat ggttggataa gctgtagtga
     6301 gcgaatgcct gaaaagggcc agaacgtgct tatttcggtg aatttcgata gctctctggt
     6361 tgaaccgcta atatgctccg cacgctatac cggaatcacc tttcggcgcg gagatgtaac
     6421 gattaagccg ggtaatggta ttgagcaagc aactcactgg atgccgctac cagaacctcc
     6481 gcaggagtcg aagagtgaat aaagcagagt tatttcagaa aatatcggct ctcgcgactg
     6541 aatgccacgc tatagcatct gagcttgatg ttggcgatga acgaaccgag atgttcgaaa
     6601 tatacagtgt gctgcgcaat ctctgtcggc gtggctacgc cactcaagta gggcgaatga
     6661 ctaacccact actctcatcc tgtgatgagg atgactcgga tgaggatgac gaatgatgca
     6721 taaatcagta gccggtgagt ttcagaagga agtcgataat actaccgttc tattggacga
     6781 tattttaagc attctcgcgc ttcttgagac tggcgattgg tcagaacatt gcactaaaac
     6841 ggagttaggc ggtcggcttg aaagagagat tacacgactt attggtgatg cgcaggaagc
     6901 tacagtcact agttatgagt taatagctga agcctggcgt ttgatggatg gacaagatcc
     6961 taaaaccagc gattggcata gcaaggcttc gaagtattta aattccaata gggtagaaaa
     7021 agttgatgat gttaaaccga agccagtaga ccacggtttc cgcgataact gcgaatgctc
     7081 tagttgccag accacggccc gtatttgttc cgaattgaca gataagtcca gcctaatcta
     7141 cgaagttaat gtaggcggta atacatgggg cgaatgcaca agagctgcat acgtaagagc
     7201 aaaagacaat ggggaattaa ccagagttgt taccaatcac ccaaataatg agcttaaaga
     7261 tcaccaaatt cgagaactag tgaatgagtt gcgggatatt gcggttacgt atcacggaac
     7321 gcagcaattg cgggagaaaa ttgccagagc agtgaacaac tcgctcaaag gtggacaatc
     7381 ccaaccgaaa cgtatacgaa ttacacatat aggtgaaaac ggaacagaaa tcccccaatt
     7441 ttctgttggg gacatctttg atgtacatgg agaagagttt ggcgagcctc acatcactat
     7501 gcctagtggc catgtttatt ggctaagagc tatggagaaa tatggtgata aatgggaagt
     7561 gttatgaggg atgcgaagtt atgactaaat ttggaaagct ggaagctcat ttgctaactc
     7621 gtaactaccg ctatgatgtg catccatata gacagtggat cgatgaaagc gaaggagttg
     7681 ttacattccc tctatacagg tttgatggga tgttggccgg gtatcagaca tacagaccag
     7741 gcgctcctaa gcagcatagc aatccgaaga tggcccggta tttcacacga gcgcacggca
     7801 gacagctact atggggaacc tatcttcctt taaaagatgg accgatatgg ataactgagt
     7861 cgatttttaa aagtgcagca gtgcacaacg cgggcggaaa ttcgtgggcg ctgttaggtt
     7921 cgactttctc agcaggatta cgtcgtcaac tggcaatgct gccgtatgac tttcgcgtaa
     7981 taggtgacaa tgatgcggct ggtgaatcac ttgtgaagtc tttcggaaaa ggttttgtgg
     8041 ccccggatct tgacgagcta caaccaaatg aagtgtctca tttgattttt agccatagcc
     8101 aataagcatc ccttctccat taaggccact agttgtggcc ttaaacaatt tgttttctac
     8161 cttttcttgt tcgagaaaat aacgttcctt tgtctggtgc tatgtaccga tcagttgcac
     8221 tgagtgagct tctttcatgt atactgtgta aatgaacagt attgagggca aaacgctatg
     8281 ggcttccctt ctcctgcggc ggattatgct gagagccgta tttctcttga tcagcagata
     8341 attagacatc cttcagcgac ctacttcatg agagcagctg atagtcatca tcgtgaggga
     8401 atattacagg gtgcattgct ggtggttgat tcctcactta ccccggttga tggttctctg
     8461 cttgtatgcg ctctggatgg ggaatatcgc gtaaaaagat accggaagtc tccacgtcag
     8521 catctggagg atttaagaac cggtaagaag gaagcattgc caaaggatga cgatggatgc
     8581 acgggcagca atgccgtgtt tggtgtgatc actcacatta tcaacgacgc aagaagtggc
     8641 gagtttgatg attgtcctgt gatgtaggag aactgattag gcggtgcaat gcaccgcctt
     8701 tttatcacac tgcgcggaat gcgatttcgc caggtattac ttcaccttgc caatacattt
     8761 gggcagcaac gcgatctgcg aggtcacgat aaatagccgt aaattcgcta tctggacgac
     8821 taataacggt tggtgttccg ttatccagat cttcacgaag agagatatga agtggcattt
     8881 ggcctaacaa ctgcgtgttg tatttctcgg ccagtttctg tgctccaccg gtgccaaaaa
     8941 ttggctcgtg atgaccgcag ttactgcaaa tatgcacact catgttttca acgataccca
     9001 gtaccggcac ttcgactttt tcgaacatca caatgccttt cttcgcatcg atcagcgcga
     9061 tgtcttgcgg cgtagttacc acaaccgcac cagttacagg aatgttctgc gccagcgtca
     9121 attgaatatc accagtgccc ggtggcatat cgagaacgag atagtccaga tcaggccaca
     9181 gtgtttcctg caacatctgc atcagcgcct tgctcgccat cggtccacgc cacaccattg
     9241 cattgtcatc ggtgaccaga taaccaatag agttggttgc caggccatga gacatgatag
     9301 gtgccatgtg agtaccgtcc ggtgaggttg gacgttggtt ttccgcgccc agcatggttg
     9361 gaattgatgg accatagata tcggcatcca aaataccaac tttcgcacct tcagcagcca
     9421 aagccagtgc caagtttact gctgtggagg atttacccac gccgcccttg cctgagctga
     9481 cggcgataat gttcttaacg ccattaatgc ctggttggtt tttgacgcgc ttaagcgtgg
     9541 caatgttgta cgacagcttc cagtcaatag cctttgcgcc agtgatacgg agcagatcac
     9601 cactacattg ctctttcagg tcttcaaaag gcttattcca cacgaaaggc atgattagtt
     9661 cgacatgcag tgtgtcatcc atcaacgcaa catggtgtaa cgctttaagc gtagtcaagt
     9721 tgtgtttcag ggttgggtgc tgaaaattag ccagcgtacc ggctaccatt gctctcaggg
     9781 catccggcga tttggactcg ctcatcccgt ctcctttatt ttaatttgcg caattgtcgc
     9841 cttgtagtgt actccagcta cgacatttaa tcatttatga gaaatgctgt tatcacatgg
     9901 cagacataag gccattttgt tactatcaag ccccttttca ctacaaagaa gtaatgccta
     9961 ctatgaccca agtcgcgaag aaaattctgg tgacgtgcgc gctgccgtac gctaacggct
    10021 caatccacct cggccatatg ctggagcaca tccaggctga tgtctgggtt cgttaccagc
    10081 gaatgcgcgg ccacgaggtt aatttcatct gtgccgacga tgcccacggt acgccaatca
    10141 tgctgaaagc acagcagctt ggtatcacac cggaacagat gattggcgaa atgagtcagg
    10201 aacatcagac tgatttcgca ggctttaaca tcagctatga caactatcac tcgacgcaca
    10261 gcgaagagaa ccgtcagttg tccgagctta tctactctcg cctgaaagaa aacggtttta
    10321 ttaaaaaccg caccatctca cagctgtacg atccggaaaa atgcatgttc ctgccggacc
    10381 gttttgtaaa aggcacctgc ccgaaatgta aatccccgga tcaatacggc gataactgcg
    10441 aagtctgcgg cgcgacctac agcccgactg aactgatcga gccgaaatcg gtggtttctg
    10501 gcgctacgcc ggtaatgcgt gattctgaac acttcttctt tgatctgccc tctttcagcg
    10561 aaatgttgca ggcatggacc cgcagcggtg cgttgcagga gcaggtggca aataaaatgc
    10621 aggagtggtt tgaatctggc ctgcaacagt gggatatctc ccgcgacgcc ccttacttcg
    10681 gttttgaaat tccgaacgcg ccgggcaaat atttctacgt ctggctggac gcgccgattg
    10741 gctacatggg ttctttcaag aatctgtgcg acaagcgcgg cgacagcgta agcttcgacg
    10801 aatactggaa gaaagactcc accgccgagc tgtaccactt catcggcaaa gatatcgttt
    10861 acttccacag tctgttctgg cctgccatgc tggaaggcag caacttccgc aagccgacca
    10921 acctgtttgt tcacggctat gtgacggtga acggcgcgaa gatgtccaag tctcgcggca
    10981 cctttattaa agccagcacc tggctgaatc attttgacgc tgacagcctg cgttactact
    11041 acactgcgaa actctcttcg cgcattgatg atatcgatct caacctggaa gatttcgttc
    11101 agcgcgtgaa tgccgatatc gtgaacaaag tggttaacct ggcctcccgt aatgcaggct
    11161 ttatcaacaa gcgttttgac ggcgtgctga caagcgaact ggctgacccg caactgtaca
    11221 aaacctttac tgatgccgct gaagtgattg gcgaagcatg ggaaagccgc gaatttggta
    11281 aagctatccg tgaaatcatg gcattggctg acctggctaa tcgctatgtc gatgaacagg
    11341 ctccgtgggt ggtggcgaag caggaaggtc gcgatgcaga tctgcaggcg atttgctcta
    11401 tgggcattaa cctgttccgc gtgctgatga cgtacctgaa accggtactg ccgaaactga
    11461 ctgaacgtgc agaagcattc ctgaatacag aactgacctg ggatggtatc cagcaaccgc
    11521 ttctgggcca taaagtgaat ccgttcaagg cactgtataa ccgtatcgat atgaaacagg
    11581 tggaagcact ggtggaagca tccaaagaag aagtgaaagc cacagctgca ccggtaactg
    11641 gcccactggc tgacgaaccg attcaggaaa ccatcacctt tgacgacttc gccaaagttg
    11701 acctgcgtgt ggcgctgatt gaaaacgcgg agtttgtcga aggttctgac aaactgcttc
    11761 gcctgacgct ggatctcggt ggtgaaaagc gtaatgtctt ctcaggcatc cgttccgctt
    11821 atccagatcc acaggcactg attggtcgtc acaccattat ggtggctaac ctggcaccac
    11881 gtaaaatgcg cttcggtatc tctgaaggca tggtgatggc tgccggtcct ggcgggaaag
    11941 atatcttcct gttaagcccg gatgctggtg ctaaacctgg ccaccaggtt aagtaattaa
    12001 acaacctctc ttcgcaatgc ccggcaacac gccgggcatt ttcatataga cacaatccct
    12061 cctgatttac acaatcccta aaacaatttg ttttctacct tttgttgatt gagataataa
    12121 gccacataag aaaacaactt gttttggagt agttgaaatg ttgaacatta cccaagccta
    12181 ccaggtggca ttgatcagtt catctcatac atcagaggaa gatgctcgcc gattcatgat
    12241 cgcaagcaat gagctggatt gggtttcaca aattgagtac ggttgcattg tccacgccgg
    12301 gttacaggac gatgactgga aagatgagtt gcgtcgatat ggtatatccg agggtgcgat
    12361 tgcgaacatt cagaaagtac ttgatgtagg ctttaactct gtacattttg attgtggcgc
    12421 accggtcgtt gaagggcttg agtgctggca atggtaatcc ggcaatcgct tcatactaga
    12481 taacggagaa tttatgctta aagaagactc atatctacta aaggcgcttg aaatgagtgg
    12541 cgttgaacct acagtgaaaa acgctcacga tatctacctt cagttttcga cagagctgaa
    12601 acagctcata aataaatatg gagtgaacga ctccagcgtc aaagccatga tagatgatga
    12661 attagaccat ctcatctaat caaaccgttg ataatggcgg tcacaatttg attcttttct
    12721 ataagaaccc gttgatcgcc actactaccc tcccatacca gcaatcacat cattaaatat
    12781 caataggtta tggttgttta taccaataag aaaacaacta aataacagat caacgaaaca
    12841 tcaacgcttt ctcccgattt ggtctaaaaa attgacgtta tcacacatca cttcttatac
    12901 ccgtaattct acgcagcaga tagcgccaca gcgtccaaat ttcaccacag acgactcaac
    12961 acactaccaa caaaaacaca cgctcaaaat cgctcccgtt gcgttacaca accatataaa
    13021 caacttattt tcaggcataa caaaacaacc aaataacaca tatgccatta cccaaaacaa
    13081 ccaacctctt tatggcaggc taccgaaaag acccacctct tctccccagg ctaccggaca
    13141 accaacctaa ctttcacagg caatccgaaa acattcatac gcgcgatacc cacgacacgc
    13201 cattacccaa acagcgaaga aacacctgaa ccgctgaaac cctaacgaag cctgtaaccg
    13261 caaggtttct ctcaatccca aaagacacga aacataacaa tcacgggaaa cgccattacc
    13321 caatacacga ataactacaa aagccttcac acactccaga caaacaactc aaacacaata
    13381 acgaaagcaa tcgcccacct gccattactc catacacgga gaagaacaat caccaaaacg
    13441 acgagaacac ccccatagaa gaaccaccgc aaaactaacg aaaccacata caccgacaga
    13501 gaaaacaaca cacccaattc accaacagaa atagtcgccg tatagaacat tctggaagga
    13561 gtgagcatta acgcctatag agaggttgag acagataaaa cacaacatgt atagcgaaga
    13621 agccaggtat gtataagtgg agagggaagg aggtgtgtcg cctccttttt cgtatttatt
    13681 caacatctta ccatcacccc ccgtaacacc cctacggtca gccttcggtc catacagaga
    13741 aaaggttgca tacgctacag aaacggctgg ggattttcag ggaaacggtc gagttgcctg
    13801 tgtggatttc gggaaaaggc tcgatgccgg ttcaggaacg gcggcgtacc ggtatagcga
    13861 gagagttgca gctacccacc tgtaatgtgc gggaagaaga gaggcgctca cgtgcaggct
    13921 gacttgctta ccgctggatt ccctctgaat gccagataca gcgtaattgt gccagacagc
    13981 cagcgggtca caatcgattt cagtaggttt tcggggagag gattattcta cccaccagcc
    14041 ccgcggcgcg cgtagagcag aatatcgtaa atactggctc tcgtgtcgtt cccggatatt
    14101 cactgcccag cctggctttc ccgacataaa atcctttttc gcctttatct tattgcgata
    14161 ataacaccaa caagtaaaca gcatatttag acgttataga aacgaagtga ggatgaagag
    14221 atgattaaga tgcctgtgac ggttgaggtg tggggcgtgg attccctggc tgagtgtctg
    14281 gatgcagtgg ggccggagtt gtaccgcaag ttgtggtcgt tcgtcccggc agaaggggaa
    14341 tcgcccaaag ggaaggatat ctggcacctg ctgagtgaag atgaaaagcg ggagctggtg
    14401 ggcgcggtac acagcgagtt cccggacgac caagattaag agtgaggcca ccagcacggt
    14461 ggcttttgtc gctattaacc tcggggcagc gtagcaccgt aacgatatac tttgtggccg
    14521 ttttcttact ggtattgttt acgtaattca gaaaacaaca tgtttaagga ttgctttatg
    14581 tttgcaaata tcgacatcaa ccaaattaag aaattgactc aaaaagagtt tgaccagttt
    14641 tatgagctgg aaggttggtc ttccactctg attaattcaa gatgggtgct ggagctgatg
    14701 actcgtgatg acgcacctgc tttgatgatt tgcgacatgg gtgaagatgc tgactttatg
    14761 gatatgagcg aattttgtgt ggacacatac aaccgcagcc agaagtacta cttcacatgc
    14821 gatagcgaaa atgacgtgat ttctaaggtc tatcttcacc tcgtccagca ttgggacgtt
    14881 caggacttcc ttgcgatatt cgcgtaagcc caaccaaagc cagcattgct ggcttaactt
    14941 catctgtagc cgcagggaag aacctgcata tcgctcatgc gcctgatcaa tatgctttgt
    15001 agccgttttc ttactggtat tatttacgcc attgagaaaa caagttgttt acggagttgt
    15061 gatgaaaaaa tcattggttc ttggtctgga caaagaccag aagagaaaag agaagcctgc
    15121 actggttgct caattaactt tgctggacat cgttgccaat ggaacctcta ttcgtctgtt
    15181 ccgtgaaaca gcggtgtctt tcgataaaaa cacctttact cgttatgtaa tgaatgttcg
    15241 tcgccagcgt ggaaaaggct ggatggcatt tcaaagaatg tggccggaac atcagctcga
    15301 actggctttg atggaagtta accgcgtggc ccagcaagag attcagagag catcagtgat
    15361 ggcaatagcc tgataatgtg caagtggtaa ttagtcgaca gtacgacagc cccgccatcc
    15421 ttacggggtt ttttgtattg taagtacata cctatgtcta taaaatagaa aaataaccaa
    15481 gacactatgg agagtgatat gagcgttaaa cgagaaaacc tgacagttga tgtctactat
    15541 gcttcagaaa gcgccgaagg taagaatgta gctaaaatca ccgtcgttac gtacaacacc
    15601 gaaactggaa ctgaagttca ggcaagtact attgtgcgta aaggagatac atcaggcggg
    15661 gaatatgagg cgcaatatca gtccattttt gatgcaacgg acccgttgtt gttgaaaatt
    15721 gaaagttact tccgtaaggt gaataaagag gtgtttgaaa ccatgatgaa tatggttaac
    15781 accgtattcg cttccagtct gaacactaac actacctgga ttggtcagta tggtctgcgc
    15841 attacctccg gcattcctgc tgacacctta atccctgaaa gcgtattcgc ttaatcctct
    15901 ttgaatggcg cgtaaaccgc gccattttct tatgcccgat aacaatttgt tttctgcctt
    15961 atctgatttg tgaaaatgct ttcactgaaa gcaacttgat aaggaaacca tcatgggact
    16021 tgatatctac attgagacgc aaccaaaaaa cgatctgaat aacgatgcat ccagaaagaa
    16081 ggttgcttac ttccgtaagt tcaatgctct ctttgggtgg atggaacgca acgtaggtga
    16141 agtcaaaaat tgtgagcttt tagaattaac gatgaatgac atttgtgctc tgaaagctca
    16201 tttgatgcac atgaacgaaa gtaactgcga agagtactta cctacctgtg aaggtttttt
    16261 cttcggcagt caggagtacg atgaagggta ctggcatgac gtgggggagt tgaaaaaact
    16321 tgtggaagag ctgattaaga accacgactt tcacaataac agactgacct tctgcgcctg
    16381 gtggtaaata tgggcgattt caagaaacgc ctgaaggaaa gagccgagat agtcagaaag
    16441 caaaacgcct cttctgtcat cagatacgca aggcagttta gtcgcaacaa caaatcagtt
    16501 gaggaaaaga tccttagcgt aatcggtcga taattattaa ggccaccact gctggtggcc
    16561 ttaaatgacc atcctgtttc ccgcaggcta aaaacaccaa cctcttacct ccaggcaacc
    16621 gacaaaccca cctgttcccg tccggctacc gcaactttcc actttgacgc cttattcgta
    16681 caacgataat taacgccaac aagaaaacaa tttgttattt acgataagga attaatcatg
    16741 aattttatcg ctactgtaaa cgcacccgca catggcaata tcgctgtaac gttctctgac
    16801 attgaaaaac gagtacttgg tgcatggcgc gacaatgaga cggtagaact gtcagcacaa
    16861 gaaaaatgca ttattgcacg cgacatcatt ggcaatcgtc gttactcgcg ggtatttgag
    16921 aaagcgtatg tggtaaattc tggattcgga acgttcgtct ttccggtgcg ctccgggcga
    16981 ttctgccagt ccaagctgat tgagttcgct acgcagattt ctgtctggat taaaactcaa
    17041 tcgtcgttca aattttccga cgatgaagca gtatcgcagg ggatgcggat cgccaacaat
    17101 gcaattaaat gcaaaaacat tacgtatacc gctggcgttg atacatggaa actgttttgc
    17161 gctaacttta tgctgaatgt atacgcaagc aaccgcatcc acatccttga tggcgtgtaa
    17221 ctgagaagag ggccagtaac ggccctttct ctatagccac cagatgccgc agggaaattt
    17281 tcagaaacgg cgaggaacgt attcatgaac cgacggggaa cggccaggat tttttcggga
    17341 aacggctgca ttcgccttta tgtagaaaaa acatcgggaa gctggtggaa tccaaccagc
    17401 ggttgtcgga caggtgagcg gggaaaatta tgatgacttt cgtcgcctga gacatccaga
    17461 tttctttcgt agcgtaatcg catacgtgat taagtggtgt gattatgtga aaaatcacgc
    17521 gcacataata cgcgagcgga tacggaacaa aacaaaatgc cgatccgcgc cgacaaataa
    17581 acgcggatca catagcaaga ctaaaagcca atgattaacc atactctata gcgcaatata
    17641 acgtgtttta agcgcgttaa tgtgttaagt aatgggtatg tactgacaag gatataaaag
    17701 cgcgtctatg gcgttatttt ggcgcttatt tttatgttgt tggagtgagt taaagacaat
    17761 aaaa
//
LOCUS       DAZPAC010000072        17596 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZPAC010000072 DAZPAC010000000
VERSION     DAZPAC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17596
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.072"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            12..514
                     /locus_tag="ACHUPM_002057"
                     /pseudo
     CDS             12..514
                     /locus_tag="ACHUPM_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="ACHUPM_002058"
     CDS             complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="ACHUPM_002058"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR0869838.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="ACHUPM_002059"
     CDS             complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="ACHUPM_002059"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR0869839.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="ACHUPM_002060"
     CDS             complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="ACHUPM_002060"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR0869840.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="ACHUPM_002061"
     CDS             complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="ACHUPM_002061"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR0869841.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="ACHUPM_002062"
     CDS             complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="ACHUPM_002062"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR0869842.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="ACHUPM_002063"
     CDS             complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="ACHUPM_002063"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR0869843.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="ACHUPM_002064"
     CDS             complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="ACHUPM_002064"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR0869844.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="ACHUPM_002065"
     CDS             complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="ACHUPM_002065"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR0869845.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="ACHUPM_002066"
     CDS             complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="ACHUPM_002066"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR0869846.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="ACHUPM_002067"
     CDS             complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="ACHUPM_002067"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR0869847.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="ACHUPM_002068"
     CDS             complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="ACHUPM_002068"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR0869848.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="ACHUPM_002069"
     CDS             complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="ACHUPM_002069"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR0869849.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="ACHUPM_002070"
     CDS             complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="ACHUPM_002070"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR0869850.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="ACHUPM_002071"
     CDS             complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="ACHUPM_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR0869851.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4371 a         4928 c         4572 g         3725 t
ORIGIN      
        1 aggaaaaata aatgggaatg attggttatt ttgcagaaat tgactcagaa aagattaacc
       61 aactgcttga gtctactgaa aaacctttga tggataatat acatgacacg ctttcaggat
      121 tacgccgact ggatattgat aaacgctggg attttttaca ctttgggctg acaggcaccc
      181 ctgcttttga tcccgcgaag aatgatccgc tgagtcgtgc cgtgcttggt gaacacagcc
      241 tcgaagatgg catagatggc ttcctgggat tgacctggaa tcaggagtta gccgcgacaa
      301 tcgaccgact ggaatcactg gatcgaagtg agctgcgtaa acagttttcc attaagcgac
      361 tcaatgaaat ggagatttat ccgggagtga ccttttcaga agagttagag gggcaactct
      421 ttgcttcgat catgctgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca
      481 agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg
      541 ccgaacatgt cggcggcttt ctgacttaca aactgatagc ttacatcagc ggcattgcta
      601 accgcacaat gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag
      661 agatcagcac cacgataccg cctgcgctgt actgccagtt tgacggtgca tcgcgacccg
      721 gttgttccgg ggcgtgcagg tacaggctca ccgccacgcg caggtagtag taaaggccgc
      781 ttgccgaacc gacaaccacg gcgcccacca gccaccacaa gtgtgcctgg acaccgaccg
      841 ccagcacgta gaacttacca ataaagccca gcgtcatcgg aataccggcc agagacagca
      901 tcatcaccgt catcaccgcc gcgaggatcg ggcgatgcca gaacagtccg cggtaagagt
      961 acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg
     1021 cgccgaggct gctaaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct
     1081 cgccagtttg cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt
     1141 aaccgagcag acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg
     1201 aggcaaaggc gataatcgcc agcaccacgc gaatcgcttc gctgtcaccc atcggtgcgt
     1261 agaggaacag acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaagaaag
     1321 tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca
     1381 gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct
     1441 cgttgagcat accgtcgcca aggtttttac ccaacgcgac aaacgacagg tcgccagact
     1501 gcgcatacac cagcgccata ccaaacagca ggaaagaaga cgctgcggca gaaaggatgg
     1561 tgtatttgat actggcttcc agtgaacgtt tctggcggaa agcgtaaccg accaggccaa
     1621 acagcggcaa agagatcagt tcgataccga ggaacagaga cgccagatgg ttggcattcg
     1681 ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactcg tccttgttgt
     1741 cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata
     1801 ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag
     1861 cgcctgcctg gccgacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga
     1921 gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg
     1981 tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg
     2041 cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg
     2101 cggagtgcga ggtatccaga atcggctgcg gatagaagcc ctgcagtacc agcagcacca
     2161 ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa
     2221 tctcgctttt cgctttaccg aagtaagcgc gatgcaacat cgccagcgaa taaacagatg
     2281 caaagaccag cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga
     2341 acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg
     2401 caaagaacag cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc
     2461 gcatgtcgcg ggtatggata cgttcataaa gctgaccaca aagaataaac agacccgccg
     2521 ccgacaagcc gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg
     2581 tgtagatagc aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt
     2641 tgatatcggt ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc
     2701 acatagcaat tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca
     2761 gcaaaccgta agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg
     2821 gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg
     2881 cgaaggcgat gaagaagccc agcattaaca ggtattccgc accactggac attggcgtat
     2941 tcagcagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca
     3001 gcgccaggat ggcaatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg
     3061 ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga
     3121 agtacatcgg caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa
     3181 ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat
     3241 atttttcgat ctctttccac gaacatagta ccgccagcac accgagcaga ccggtcagca
     3301 cgaccatcag cagcgacagc ccgtcaatgg cgagatgaat agagataccc aaacgcggga
     3361 tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat
     3421 aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg
     3481 cgatccagcg tggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccaa
     3541 taaagggaat taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc
     3601 ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt
     3661 agtgtcggat gcaccgcgaa ggcggcttat ccgacctacg ttcttatatg cccaggggca
     3721 atcccacaat ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg
     3781 atgctcatgg atgccacata ccagcgcaga tagccgttct cacttaacag cagaccttta
     3841 cctgcaaagc gggaaaggac agccggaatg ttcatcattg agttcagcgg atcgcgtttc
     3901 agcaaccagg caatacccag gaacggcctg acgaacactt tgtcgtacag ccagtcaaag
     3961 ccccaggcgt tgtaccacca ggtactcagc aggcggcccg gcgcactgtt ggcgatggag
     4021 gtcaccagag tacgtttacc cagccacagc caagctgcca gcagaatgcc gacaatcgcg
     4081 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc
     4141 ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc
     4201 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
     4261 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
     4321 accagacctg ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca
     4381 tccttactga agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc
     4441 aggaagcaga gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc
     4501 tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
     4561 aacgcgtgag tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
     4621 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
     4681 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatgcc caccagatgc
     4741 agaatttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
     4801 gttaccatgg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
     4861 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
     4921 agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc
     4981 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg
     5041 aaaacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
     5101 tcggtgtaat agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
     5161 tacatcagca gcaggttgtc ggcaagcacc agaactacca tgctggcgat gaacaggttg
     5221 gtgtaggcga agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg
     5281 tgaataagga aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc
     5341 accaggttaa aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg
     5401 tatgcctgct cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc
     5461 aggcccacag agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag
     5521 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
     5581 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
     5641 gcagcgcaag gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca
     5701 cctgaccgtc ggtctgaccc cagtagcttc cggcgacaac aaaggccagc gcggaggcgt
     5761 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
     5821 ttaagccaag aatgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
     5881 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
     5941 acgctcttca cgaccgacgt ggaaggccac aaccagacct gcgagcagca gcatagaagc
     6001 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
     6061 tggcgtaccg tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag
     6121 catgatggcc gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc
     6181 ctgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
     6241 aatggcaccc gcgtagacga taatttccag cgcaccggcg aagtaagcgc ccagtgagaa
     6301 gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt
     6361 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
     6421 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
     6481 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
     6541 tattccgggt atttgcccgg accggagatc agcagatcct ctttttcgta aaccagatcc
     6601 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
     6661 gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
     6721 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
     6781 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg
     6841 atacgaccac gataacgggg cggcagatag atcggctctt ccgggtacat tcgcgtttcg
     6901 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
     6961 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
     7021 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
     7081 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
     7141 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
     7201 caccgaagaa cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca
     7261 cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct
     7321 gctcggcttc cggctggtca aacgggtgac ggtgacatac cgccacgccc gcgatggcaa
     7381 aggtaataaa accaaagaat tgcgggataa cgttccacac atgcgcctgg ctgttgacga
     7441 tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga
     7501 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcaac gagtatttgt
     7561 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga
     7621 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg
     7681 caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
     7741 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct
     7801 ggagcgaacc gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca
     7861 ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca
     7921 ccgctttgag gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
     7981 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatac cggacatacc
     8041 catcggcaag cccacctgcc ctgccgtcag cccttcagcg atttcaaccg gcagcgtgac
     8101 cgtattgcca tcgtaactaa aggagacgtg tgtacctgcg ttcacaccta acttcgcggc
     8161 atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg
     8221 ctgtgacaat tcatcgctgc caaacaggtg gtaatacggc gcgatacgcc atttcccgtc
     8281 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc
     8341 aaacagacgc acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt
     8401 gttccacgcc tgcggggagt tccagcccgg cgcccaggca aacggcactt gcgaacggtg
     8461 cgcagtcggc tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg
     8521 ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg
     8581 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt
     8641 gatacctgcc agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt
     8701 ccagtccact tcacggctca gcagggtgct gtgcagcgag tgtaaccagc gccagctttc
     8761 cagcatgaca gttttgctgt cgtaataagc aggatcgtaa acctggaaga aacgttgggc
     8821 gcggccttcg ttgttgatca ccgtaccgtc gctttcagca aaactggcag cagaaagtac
     8881 cagatgggcg ttttccataa tcgctgtgcg ttgatgatca accaccatca ccagcggcgc
     8941 tttagccagc gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag
     9001 caccaccacc gcgtcggcgc gtccggtttc cagttcggtc aacgcttctt caagcgaacc
     9061 gccacccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac
     9121 gtcagcgccg cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctat
     9181 gctaccggcg ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac
     9241 gatgacgtcg attttgcttt gcagctcagg ttcgataccg tcaaccgctg gcgcagagtt
     9301 atccagcgca tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
     9361 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
     9421 cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
     9481 tgccgccatt tcgcgcgctt tacctttcac agcctgacgc actgccagcg cgacgcgcgc
     9541 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
     9601 cagagccgga gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc
     9661 ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc
     9721 aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg
     9781 cagaatatct gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
     9841 acgacgctgt actggctgac gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg
     9901 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
     9961 cagttcgccg taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat
    10021 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    10081 aaatacgccg gtcgggcaaa tttcgaccag gttaccagaa aattcgcttt ccagcgtgcc
    10141 gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca gatctgtacc
    10201 gtcagcgtaa tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc
    10261 gtgagagatg aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg
    10321 acggaagctg tgtccggtca tcacagtcat atcctgaaga tggcagttac cgccttcttc
    10381 acataccgga cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa
    10441 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    10501 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    10561 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    10621 cagagacaga caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc
    10681 tacatgaatt gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat
    10741 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    10801 tattgctgaa cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc
    10861 tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga
    10921 atcgacacag ttgctcaagt gtttcgatat cgcccggctg gccttcgcca cgctccagcg
    10981 cacgcagaat tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg
    11041 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt
    11101 caaccgccat cgccagcgcc gtacccagac ggctgcccgc tttaccgata ctttcgaatt
    11161 ccatcggcag atcaaggtgc gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc
    11221 aagctttaaa tttcagacca tcacgcatac caccggcgta atcttcgagg atctcgcgtg
    11281 cggtggtgcc gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca
    11341 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    11401 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc
    11461 atacgccgga ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca
    11521 gggagttgat taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga
    11581 acagttcgaa gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg
    11641 cttcggcaat ggcgcggcgc agatttactg ccgcttcgat atattcgcca cgcaggaaga
    11701 tgtagccacg gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt
    11761 gcggcagttg ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt
    11821 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc
    11881 cagtcgaaaa gcccgctccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga
    11941 tttcgtccgg agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc
    12001 ggtattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    12061 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    12121 cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    12181 ccccaggcag caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt
    12241 gatgttcagc tttttctcga gcgccgcctg aataccctga tacccgttga tatgacagac
    12301 cacgctgtcg caataacgga tcacatggcg accaaccggc tggcggaaga tctgactgta
    12361 gaacgttgcc acaccttcga cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg
    12421 gatcgcacca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    12481 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc
    12541 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    12601 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    12661 cggatcgccg ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga
    12721 taactcatgg tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca
    12781 atcatctgga aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg
    12841 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg
    12901 tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    12961 ctctggcgca gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacct
    13021 ccaaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    13081 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    13141 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc
    13201 gctttctcgt aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga
    13261 tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata
    13321 cggaaacccg tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    13381 aacacgggcg tcattgcgcc gacgtcctga ataaaggtcg agatgtacag caggtggctg
    13441 ttgatacgga acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg
    13501 atcccggcca gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg
    13561 tattcaatac ggtcggtata cggaatgtag ctgtgccagg actggcgctc gcccattttc
    13621 tccgcaccac ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg
    13681 agttgtaaaa cgatacggaa agccccgtgc gccgacgggt ggttcggacc gaggttgagg
    13741 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    13801 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    13861 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    13921 cggcgcaggt tcgggtgacc gtcgaaagta atgccaaaca gatcccaggt ttcacgctca
    13981 taccagttag cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc
    14041 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    14101 gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt
    14161 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt
    14221 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    14281 gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag atgatcacgg
    14341 gtctgccaag cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    14401 ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    14461 atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    14521 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    14581 tcgccaacca cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc
    14641 agtgcctgca tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg
    14701 atgaatttat cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca
    14761 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata
    14821 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    14881 gaagcacgca atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    14941 accatctcaa cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga
    15001 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    15061 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    15121 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    15181 atactgttcg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    15241 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    15301 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    15361 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    15421 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg
    15481 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca
    15541 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    15601 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    15661 cacatcgatt catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat
    15721 acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag
    15781 tttcttcaca ttagtttaca taatatcaac accattagca tttaatgccc tttcacgcca
    15841 gatccttgac gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg
    15901 gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt
    15961 attttcttga tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta
    16021 cgcattttta acatcattgt agcaggtgat ttttttcagg cgattatttg tgcgttcggg
    16081 acgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcact atgcgaggaa
    16141 ggatttttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct cgtttttaca
    16201 ctattgtctc aggaattatc tatcgtccgt cgattactcg atatcccttt caatcaacaa
    16261 tgaatcatcc ccttccggag cagacattgg gctgtattgc cacggattat ggtagctctc
    16321 catcgcctga tagatcacct gggccagctc attattactg gacgggtcat agcacagcaa
    16381 atattcggta tcaggtagcg gcggtaagcc atctactcca ctcagcacac gcaggtacgg
    16441 gctcatcatt tcaatcggcc ttgccgtcac gccaagaccg gctttcactg ccgcacgaac
    16501 ggccggaagc gtcgaggcga cataagccag tcgccatgga atatctgctt tattaagcgt
    16561 cgccagcacc atatcgcgaa acggacttgg atcatccagc aatacaagag ggatcggctc
    16621 gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg tacgcaggtt
    16681 caatgcctta aacgctgaag gacgatgggt ggttaccatc aaatccactt cctgggattc
    16741 cagcatttct gccatatagg cattacgttt aacgcggaca tccagcgcta atttcggata
    16801 aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt catctgaagc
    16861 accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg cctcatcatt
    16921 aaaacgcagt attttcctgg cgtaaccaag aagttgaatg ccatgttcag ttaacagttt
    16981 gttgcgaccg tgacgagcga acagttcttt cccaacgagt tgttccagac gctgcatttg
    17041 ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa aagtgttcag
    17101 atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta tcggacgatt
    17161 tgcacttatc atatattatc acttactggc ggctcatact gagctggtta atgctgtgca
    17221 cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc cggaacccgg
    17281 acgaaagtaa aaatgcatat gagttgcact aaaaaagcga ctcacattgt tccgttataa
    17341 tgcctgaagt agatcacaga atatatcttc agggatcgca tatctattaa gttactcact
    17401 cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc ttgttccact
    17461 ctcgtgcagc atcgctagtc atacgcgaac acgtacaaac agcaatggtg aggcggcatc
    17521 aagagcaggg atccgtcatt tatgcgagca ttttacccca aaaacctttt atttataagg
    17581 gtcattgcga attatc
//
LOCUS       DAZPAC010000073        17524 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZPAC010000073 DAZPAC010000000
VERSION     DAZPAC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17524
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.073"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(76..807)
                     /gene="rlmB"
                     /locus_tag="ACHUPM_002072"
     CDS             complement(76..807)
                     /gene="rlmB"
                     /locus_tag="ACHUPM_002072"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR0869852.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(987..3428)
                     /gene="rnr"
                     /locus_tag="ACHUPM_002073"
     CDS             complement(987..3428)
                     /gene="rnr"
                     /locus_tag="ACHUPM_002073"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR0869853.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="ACHUPM_002074"
     CDS             complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="ACHUPM_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR0869854.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4097..5395)
                     /gene="purA"
                     /locus_tag="ACHUPM_002075"
     CDS             complement(4097..5395)
                     /gene="purA"
                     /locus_tag="ACHUPM_002075"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR0869855.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5499..5696)
                     /locus_tag="ACHUPM_002076"
     CDS             complement(5499..5696)
                     /locus_tag="ACHUPM_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR0869856.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="ACHUPM_002077"
     CDS             complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="ACHUPM_002077"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR0869857.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="ACHUPM_002078"
     CDS             complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="ACHUPM_002078"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR0869858.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="ACHUPM_002079"
     CDS             complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="ACHUPM_002079"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR0869859.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="ACHUPM_002080"
     CDS             complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="ACHUPM_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR0869860.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="ACHUPM_002081"
     CDS             complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="ACHUPM_002081"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR0869861.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="ACHUPM_002082"
     CDS             complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="ACHUPM_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR0869862.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="ACHUPM_002083"
     CDS             complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="ACHUPM_002083"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR0869863.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="ACHUPM_002084"
     CDS             complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="ACHUPM_002084"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR0869864.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="ACHUPM_002085"
     CDS             complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="ACHUPM_002085"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR0869865.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16016..17155
                     /gene="queG"
                     /locus_tag="ACHUPM_002086"
     CDS             16016..17155
                     /gene="queG"
                     /locus_tag="ACHUPM_002086"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR0869866.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17138..17191)
                     /locus_tag="ACHUPM_002087"
     CDS             complement(17138..17191)
                     /locus_tag="ACHUPM_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869867.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17425..17500)
                     /locus_tag="ACHUPM_002088"
     tRNA            complement(17425..17500)
                     /locus_tag="ACHUPM_002088"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17465..17467),aa:Gly,seq:gcc)
BASE COUNT         3972 a         5005 c         4398 g         4149 t
ORIGIN      
        1 tcaccgtaat tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt
       61 cataattcag acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc
      121 aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca
      181 atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaagcg
      241 tccggtcatt ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca
      301 gatattctct tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg
      361 aacgctttct gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg
      421 atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc
      481 gaggttgtgc ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc
      541 gatcagatcc ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat
      601 gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat
      661 aaccacgccc tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc
      721 ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc
      781 gtggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc
      841 actacgttta tccggcctac atgatctctg caatatattg aatttgcgtg attatgtagg
      901 ccggataagg cgttcacgcc gcatccggca tgaacaaagc gcattttgtc agcaatctaa
      961 ccctcttctt ttaaagaggg tattgatcac tctgccactt ttttcttcgc cgcacgcttc
     1021 gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg
     1081 tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct gtctggctca
     1141 aagtttacct ttttaccgac ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt
     1201 ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga
     1261 ctaaagtcga ttttgcgctc gtccatatta accgcttcga cgcgaacttc cacgcgatcg
     1321 cccaggcgat aagtctggcc gctggattcc cccatcaggc gttgccctac ctggtcaaag
     1381 cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc
     1441 agacggacga agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct
     1501 acctggtcga gcatgaagtc acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg
     1561 gcacgacgtt ccgccatcga acagtgctga cccagttgca acatctcttc catcgaataa
     1621 tggtagccgc cggtttcagt ggtgttgccc tgatgcccct gctctttcgc cagcagatac
     1681 ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca
     1741 taggactgca atgccaggcc aaagtgacca cggttttctg gatcgtaaat cgcctgtttc
     1801 atcgagcgca gcagcatggt ttgcagcatt tctgcatcag gacggtcggc aaccgactcc
     1861 agcagttccg cgtagtcacg cggttccggc ttgttaccac ccggcagctc cagccccagc
     1921 tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg
     1981 aacagtgccg gttctttcgc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc
     2041 atgcactctt caattaattt gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata
     2101 cggcgttcag cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt
     2161 tcttcacggg ctttatccag cactttatag aggttatgca actcttcgag atgcttaacc
     2221 agcgggacgt actgctcgcg cagatcctga tcgccctgca gaatatgcca gactttggtg
     2281 taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg
     2341 cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc
     2401 gaacacaggc cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac
     2461 accgacgtgc cacggttacg cgcttctctg tccagcggcg ttggcggacg cacatagtag
     2521 ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa
     2581 actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc
     2641 aaatcgacac ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc cgcaacctgt
     2701 tgctcaacag cctgcggcca gatatacgga atttcatggg tacgcagagc gatatcaacc
     2761 gccatgccgg tgcccatatt gtcgcccagc acttcgacga ttttacccac cgctttggtg
     2821 cggcgagtcg gacgctgagt cagttcgact acgaccacaa agcccatccg cgcgcccatg
     2881 atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg aaccacaaag
     2941 ccgacgcccg cttcggtaaa gtagcgacca acaatctggc tggtttttgg caccagtacg
     3001 cggacaatac gcgcttcacg acgaccttta cggtcagcac ccagcggctg cgccagcacc
     3061 tgatcgccat gaatacaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc
     3121 ccttcaaccc gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg
     3181 tcgaggcgtt ccggcagcgc atagcactga cgacgagtga agaccagttg accatcgcgc
     3241 tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt
     3301 tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg ttcgaggata
     3361 aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct
     3421 tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca
     3481 ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt
     3541 ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa
     3601 tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat
     3661 caccaatacg tatcgcactc gccggtttac ccaggcgaat gccgccattt tttccacgaa
     3721 cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg
     3781 agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg
     3841 ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct
     3901 caaaagggaa atcgggaaaa aatacattta tattgatgat aaaccagcca caagctgtgt
     3961 cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag
     4021 cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct
     4081 gccaggcgta ccagaattac gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat
     4141 ccggaccggt agagatgata tcgatcggca caccggtcag ctcttcaata cgcttgatgt
     4201 agttcagcgc cgcctgcggc aggccgctac gatctttcac gccgaaagtg gattcagacc
     4261 agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag
     4321 tggtagccac ttcgcgacca tccggcatac ggtaagccac gcagagttta acctctttca
     4381 ggccatccag aacgtccagc ttggtcaggc aaaagccaga cagggagttc agctgtaccg
     4441 cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc gtagttgcgc
     4501 cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga
     4561 acggacctgc acctacacga gttgagtaag ctttgaggat acccagaaca taatcaacat
     4621 aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta
     4681 cgtacggata agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga
     4741 cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc accatagaag
     4801 tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag
     4861 ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag
     4921 cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgct actttatctt
     4981 cataagcagg cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct
     5041 cacgcgcgtt atccagcgca acgtgataat caaggatcag cggacatgct tcagacagca
     5101 gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg
     5161 cggccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac
     5221 ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt
     5281 tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga tcgacgatct
     5341 tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc
     5401 aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt
     5461 ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac
     5521 atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta
     5581 tcgggcaaat tggtcatcgc agagatcatc ttcttccatg ccttcgggta aagcatcggc
     5641 cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga attcattcat
     5701 tatccttata gaaaaagaaa accaccgaca tccctgagga tgcggtggct ttattgacct
     5761 gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa
     5821 tcgctatccg ggctcatgac catcacgtcc tgattgccag agaagctgtt ctcataagca
     5881 cgcaggctac ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc agcaaacagt
     5941 ttggctgctt cggcatcgcc ttcaccacgc atgatgcggc cctgacgctc agcttctgcc
     6001 agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct
     6061 tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt gtagatcgct
     6121 tcagacactt cggtcggcag gttgatctgc ttgatacgca catcgacaac ttcaataccc
     6181 agcgccgcca tactgttcgg gttgatgacc ggaactttgc ccttcgtctc agccgttacg
     6241 cgctctgccg cttcggcaat ggcgttatct gccgccgggg tagtaacttc atcttctgta
     6301 cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc acgggaatcg
     6361 gtgacgatgt ctttcacgtc caggcgacca atttcagaac gcagacggtc agagaactta
     6421 cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag gtagtaacgg
     6481 ctgaaatcgc tgatgcgcca tttgatgtaa gaatcgacga tcaggtcttt cttctctttg
     6541 gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag cattttcacc
     6601 gtttcaatga acggtatctt gaaatgcaga cccggctcat aaaccagagg tttgttgtca
     6661 tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc tttgacgaca
     6721 aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac tgacttacgc
     6781 atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca
     6841 taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg gaggaagagg
     6901 ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca ggggcgttac
     6961 cgcctttcag catctggtct aacggcagaa ccatcaggtt gccaccttta tcgttaacca
     7021 gcactttgcg ggtgttaccc aacacttttt ccatcgtctc gatatacaga cgctcgcgag
     7081 taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc acttcaccct
     7141 gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct
     7201 gaccgttcgc acgcggctga acttcgttgg tatacgcttc tgcttcacga atgtattgct
     7261 gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct tccggcggac
     7321 gagctgcctg gaagttgacg tccagcagag tgatacccat gtcatacgga cggatagtct
     7381 cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc agaatgcggt
     7441 ccatggtgta tttaccgata actccacgca gggcgctgtc ggtagcctga cgcaggctgt
     7501 catccgggct ggtcacgcta tacagatatt tttccggatt ggtgacgcgg tactgcacgt
     7561 tcatctcaac gcgcactacg ttctcgtccg acgtcagcat cacaccagaa gcggccagtt
     7621 cacgcacggc ttccacgttc accggtttga cttcgtcgat aaacgtcggt ttccagttca
     7681 gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgccg cgttcggctt
     7741 ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc gctgcgatgg
     7801 taacgacacg accgccaagc tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc
     7861 cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg aagatatcat
     7921 ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca gagttgccgc
     7981 caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc tgattccacg
     8041 ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga cgccagagga
     8101 tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa cgccggttct
     8161 tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact tacgctgccg
     8221 tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact tctcagacgt
     8281 ccttcctgcg gcggcaaacg caatgtatgc tgcgccacct cgccggaaag ccgctccgtc
     8341 aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg cggaaagcca gacacggatc
     8401 ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat cttccagcat atcgatcttg
     8461 ttcatcacca gcagggttgg gatctcgtga gcgtcgatct cttcaagaac cgtattcacc
     8521 gcttcgatgt tttcttgtac acgtacatcc gccgcatcaa tgacgtgcag cagtaatgtg
     8581 gcttgccgcg tctcttgtaa cgtggcttta aatgctgcca ccagatcgtg cggcaggtgg
     8641 cgaataaacc ctacggtatc tgcaagtacg gtttcaccga catccgcgac gtcaatacgc
     8701 cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg
     8761 atgcgattga aaagggtaga tttaccggcg ttggtatatc ccaccagcga aacagtagga
     8821 acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac tctttctagg
     8881 cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc gagctgggtt
     8941 tcacccggac cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt ccagccacgc
     9001 accaggcgcg tagccagatg gcgcagctgc gctagctcaa cctgcaactt accctcatgg
     9061 gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat aacacgacac
     9121 tcgcacaaac gctccaggtt acgctcttgc gccgggctca gggcatggtc aaaaagaacg
     9181 accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa ctgctttacc ttcacctaca
     9241 aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc ttcgacaccg
     9301 gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa
     9361 tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa gcgtataacc
     9421 ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc
     9481 gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc
     9541 atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg
     9601 agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa
     9661 caggatcacg aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa
     9721 ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga
     9781 ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat
     9841 tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa
     9901 caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc
     9961 cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac
    10021 ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat
    10081 aacccacgca acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc
    10141 ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat
    10201 ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt
    10261 ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg
    10321 cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct
    10381 cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg
    10441 cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat
    10501 acaacatcgt accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt
    10561 ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat
    10621 ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat
    10681 ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac
    10741 gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct
    10801 taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa
    10861 tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc
    10921 acgtccgcca gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga
    10981 tttcgtgcaa tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag
    11041 cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc
    11101 ctgatggtca catgctgtgc atctgactgg aaatcaatac ccaattccgc cagggcagac
    11161 tgcgcttttt ctaatgccga tttttcttcg ccagaaactt ttagccgcaa cggaataagc
    11221 agcggctgag cgcaaactgg cgcttcaccc ggcgtcagtt gtacctgacg cagccaacgt
    11281 tctgccactg gcaaggctaa aagtgaaatg ttgccgtcgc gctccagcaa cgcacagtcg
    11341 gaatggacga tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc
    11401 tgcggttccg gcgcttttaa tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac
    11461 acttcacctt gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga
    11521 ctgcctgatg ctggcgcagg agtgtagcgc ggagctaccg gctcacgagc tgccggttct
    11581 gcaaagtgat tgcgccccgc cgccacgcgg ttttccggaa tggaacgcgg tgcaggttgg
    11641 ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc
    11701 tgatagataa aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg
    11761 tgcacgttga cgtccacctg atgcggatcg atctccagat acagcacaaa tgccggttgc
    11821 tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg
    11881 cgcatcatgc gaccgttcac gtagcaatac tgaatttctg ccagtgcggg cgtggtgtga
    11941 tttggatcgg ccacccagcc gcgtagcgtg agatcgccgt gttgccattc aatcgccagc
    12001 gcttgttcaa gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct
    12061 tccggcactg cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg
    12121 tcgaaacgcg ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa ttcggttttc
    12181 tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc
    12241 gtcgtcccca caggatgcgc cgccggtttt accgtcacgt tcatatcgcg cccttcggca
    12301 taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc
    12361 gaactgatac tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg
    12421 tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt
    12481 ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca
    12541 atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac
    12601 gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc
    12661 tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg
    12721 gcagtttgtg ccgttgcccc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg
    12781 cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa
    12841 cgttcttcgc tgttgttgct gataaaaccg gtttcgacca gtactgacgg gatatccggc
    12901 gaacgcagaa cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt
    12961 tgcaactgac tgatcatact ggtcgctaca tcatacccta cccgctggga atgaccgaac
    13021 tgtaaatcca gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatcg
    13081 cccgccccac ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttcg
    13141 ctgttggcgc gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt
    13201 gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcgctg
    13261 cgccccatca ccgagataaa gtaatccccg tcacgggtta aaacgccttt aaacatcgga
    13321 tcgtcattga gcaaagtgcg caatttacgc gcgatggcga tggtgacatt tttctcccgc
    13381 gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg
    13441 ataattttat cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg
    13501 ctgataacac ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg
    13561 acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggtggagga
    13621 ggcggtggcg gcacatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc
    13681 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
    13741 gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac
    13801 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
    13861 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta
    13921 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc
    13981 agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
    14041 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
    14101 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
    14161 ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc
    14221 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa
    14281 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
    14341 cccagagctt gtaaaaaacc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
    14401 agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
    14461 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
    14521 gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa
    14581 acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac aggcggcatc
    14641 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
    14701 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
    14761 ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg
    14821 tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
    14881 ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
    14941 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
    15001 tgcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
    15061 ttccaggctt tcggtaagag agtccatcgt cagttcatgc accatcaatt ccggtcgtgc
    15121 agtcagcagc ggcgcaatgt tttcactgcg ggtcagtact cggactaaac cagcaccagc
    15181 acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat
    15241 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg
    15301 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
    15361 cagccccagt gagtcaaaat gcagttgtcc tgtaacatcc cgcgcttttc cagtaagcaa
    15421 gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt
    15481 agcgccggtt tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg
    15541 ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
    15601 accgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
    15661 gatctcgcca cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
    15721 tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc
    15781 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
    15841 gtcaggatac gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc
    15901 atagagtgtg agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
    15961 gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
    16021 agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg
    16081 ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc
    16141 atggctggac aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg
    16201 cgctcgcccc catgagttat taccaggtac gctgcgtgta atcagcgtgc ggatgaatta
    16261 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
    16321 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg
    16381 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
    16441 tattctcgag cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact
    16501 tatcctcaat cgcgaggccg gttctttctt ctttttaggc gaattgctgg tcgatattcc
    16561 gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcatagcct gtatgacgat
    16621 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
    16681 tctcaccatc gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg
    16741 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
    16801 tacagaagac gatttcagcc cgcgtaagcc gctacacgca caggaactca ttgagttatt
    16861 tgcctggagc gaagagaagt ttttaaaagt cacggaagga tcggcgattc gccgtatagg
    16921 tcacctgcgc tggctgcgta atatcgccgt ggcattaggc aatgcgccct gggatgaaac
    16981 gattctggcg gcgctggaga gtcgcaaagg tgagcaccca cttcttgatg agcacatagc
    17041 gtgggcgatt gcgcagcaaa tcgagagacg aaatgcgtgc atagtcgaag tgcaattgcc
    17101 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
    17161 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgcataa aatcgtcaag
    17221 tgatcgactc gacaaaacgg aatcttgatt tttaacttat ttatcaatag gttaaaaaaa
    17281 taccattcac cgtgcgaagt ttctttgaaa ttaagattac agcctgagga tgtggataac
    17341 tctgtttaca agagtttgtc agagacaaca aaaaccagca ataacgcatc gcgtcgctgt
    17401 ggatatttta ttgagagaag aatttggagc gggaaacgag actcgaactc gcgaccccga
    17461 ccttggcaag gtcgtgctct accaactgag ctattcccgc ttgggtggtc tgtgccttac
    17521 agca
//
LOCUS       DAZPAC010000074        17420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZPAC010000074 DAZPAC010000000
VERSION     DAZPAC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.074"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            77..1053
                     /gene="ybeQ"
                     /locus_tag="ACHUPM_002089"
                     /pseudo
     CDS             77..1053
                     /gene="ybeQ"
                     /locus_tag="ACHUPM_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(1123..1605)
                     /locus_tag="ACHUPM_002090"
     CDS             complement(1123..1605)
                     /locus_tag="ACHUPM_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR0869868.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1840..4422
                     /gene="leuS"
                     /locus_tag="ACHUPM_002091"
     CDS             1840..4422
                     /gene="leuS"
                     /locus_tag="ACHUPM_002091"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR0869869.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4437..5018
                     /gene="lptE"
                     /locus_tag="ACHUPM_002092"
     CDS             4437..5018
                     /gene="lptE"
                     /locus_tag="ACHUPM_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR0869870.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            5018..6049
                     /gene="holA"
                     /locus_tag="ACHUPM_002093"
     CDS             5018..6049
                     /gene="holA"
                     /locus_tag="ACHUPM_002093"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR0869871.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            6051..6692
                     /gene="nadD"
                     /locus_tag="ACHUPM_002094"
     CDS             6051..6692
                     /gene="nadD"
                     /locus_tag="ACHUPM_002094"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR0869872.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6716..7327
                     /locus_tag="ACHUPM_002095"
                     /pseudo
     CDS             6716..7327
                     /locus_tag="ACHUPM_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            7587..7904
                     /gene="rsfS"
                     /locus_tag="ACHUPM_002096"
     CDS             7587..7904
                     /gene="rsfS"
                     /locus_tag="ACHUPM_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR0869873.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7908..8375
                     /gene="rlmH"
                     /locus_tag="ACHUPM_002097"
     CDS             7908..8375
                     /gene="rlmH"
                     /locus_tag="ACHUPM_002097"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR0869874.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8406..10307
                     /gene="mrdA"
                     /locus_tag="ACHUPM_002098"
     CDS             8406..10307
                     /gene="mrdA"
                     /locus_tag="ACHUPM_002098"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR0869875.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10310..11422
                     /gene="mrdB"
                     /locus_tag="ACHUPM_002099"
                     /gene_synonym="rodA"
     CDS             10310..11422
                     /gene="mrdB"
                     /locus_tag="ACHUPM_002099"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR0869876.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11433..12521
                     /gene="rlpA"
                     /locus_tag="ACHUPM_002100"
     CDS             11433..12521
                     /gene="rlpA"
                     /locus_tag="ACHUPM_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR0869877.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12660..13871
                     /gene="dacA"
                     /locus_tag="ACHUPM_002101"
     CDS             12660..13871
                     /gene="dacA"
                     /locus_tag="ACHUPM_002101"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR0869878.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13981..14244
                     /gene="ybeD"
                     /locus_tag="ACHUPM_002102"
     CDS             13981..14244
                     /gene="ybeD"
                     /locus_tag="ACHUPM_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR0869879.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14345..14986
                     /gene="lipB"
                     /locus_tag="ACHUPM_002103"
     CDS             14345..14986
                     /gene="lipB"
                     /locus_tag="ACHUPM_002103"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR0869880.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15246..16199
                     /locus_tag="ACHUPM_002104"
     CDS             15246..16199
                     /locus_tag="ACHUPM_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR0869881.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16408..17373
                     /gene="lipA"
                     /locus_tag="ACHUPM_002105"
     CDS             16408..17373
                     /gene="lipA"
                     /locus_tag="ACHUPM_002105"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR0869882.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT         4361 a         4396 c         4463 g         4200 t
ORIGIN      
        1 cataaaataa ggcttacgca aaaatggaac acataaaaat ataatatgtt ttatctacaa
       61 ccataaggaa atgataatgg tttttacgtc aagttgctgc gataatttat caatagatga
      121 gattatcgaa cgtgctgaaa aaggcgattg cgaggctcaa tatattgttg ggttttatta
      181 taatcgcgat agcgcaattg actctccgga cgacgaaaag gccttttact ggctgaagct
      241 ggccgctgag caaggtcatt gtgaagcaca gtattcctta gggcaaaagt ataccgagga
      301 taaaagccgt cataaagata atgagcaagc catcttctgg ctgaaaaaag ctgccctaca
      361 aggccatact ttcgcttcca acgcccttgg ctggacactg gatcgtggag aagcccccaa
      421 ttataaagaa gcggttgtct ggtatcagat agccgcggag agcggaatgt cttatgcgca
      481 aaataatctt gggtggatgt acagaaatgg caacggagtc gcaaaagact atgcgctggc
      541 atttttctgg tacaaacaag ctgcattaca aggccatagt gacgcgcaaa acaatctggc
      601 cgatctttat gaagacggaa aaggcgttgc tcaaaacaag acactcgccg cattctggta
      661 tttgaaaagc gcacagcagg gtaatcggca cgcccagttt caaattgcat gggattataa
      721 cgctggcgaa ggggtggacc aggactataa gcaagcgatg tactggtatc tgaaggctgc
      781 cgctcaggaa agcgtcggcg cttacgtcaa catcggttat atgtataaac acggacaagg
      841 cgttgagaaa gattatcagg ctgcctttga atggtttacg aaagccgctg aatgcaatga
      901 cgccactgcc tggtataacc tggccattat tatcactacg gagaaggaag acctgtcgat
      961 ctccgacagg ctctcgacct gtatcgtaaa gttcagtcat ccggaaccag agatgtcagt
     1021 caggaaattc gtgagactga agatttactg tagagataaa aacaagggcc ggggaatttc
     1081 attcggccca tattctcttt attgctgtgc gaaatcagac tattacggct caaacgggcg
     1141 tctctggaac tggtcatgac cacatttcgg gcatagcgtc agcacttccg gtgtgtagat
     1201 cgggagatgg aagtgacatt tctcgcagac cagatttccc agcccgacca cttctccgct
     1261 gtgataaacc ccatgatgat tgaggtcctg gaaaacttcg cgccattcaa gctgcgtttt
     1321 atcggtgata tctgccagct cctgccataa gctttcttta atcacccgca taaagacgct
     1381 gtcagattct tctttcaggc tctcttcata gctcatggcg aactcttcca ggtcacgtct
     1441 tacagctcgc gtcagctcat cgacctcggt tcgcgttaac tcccctgttt ttattacgcg
     1501 ctcgcgcgcc tgttccacca gtgcgtcgat atcacgttcg ccattgcgca ggcgttcgct
     1561 cagtgacgcg accagttcac ggtaatattg agcaaccttg ttcatcgttt catctcccgg
     1621 gtcgttaact gactataata atagacgttc tttgttcctt gctttgctaa tacgcccaca
     1681 aactctgtaa attaatgtat gaataatccg ggcatcattt caacgtcgag tgcgcgaaag
     1741 gctgttttga cgcggacgtt tgggctatgc tatgcggatc tgaaaaacca cctcaacgct
     1801 acatttgtag ccgtattgaa aacaggacca ctggctgcca tgcaagagca ataccgcccg
     1861 gaagagatag aatccaaagt acagcttcat tgggatgaga agcgcacatt tgaagtaacc
     1921 gaagacgaga gcaaagagaa gtattactgc ctgtctatgc ttccctatcc ttctggtcga
     1981 ctacacatgg gccacgtacg taactacacc atcggtgacg tgatcgcccg ctaccagcgt
     2041 atgctgggca aaaacgtcct gcagccgatc ggctgggacg cgtttggtct gcctgcggaa
     2101 ggcgcggcgg tgaaaaacaa caccgctccg gcaccgtgga cgtacgacaa catcgcgtat
     2161 atgaaaaacc agctcaaaat gctgggcttt ggttatgact ggagccgcga gctggcaacc
     2221 tgtacgccgg aatactaccg ttgggaacag aaattcttca ccgagctgta taaaaaaggc
     2281 ctggtatata agaagacttc tgcggtcaac tggtgcccga acgaccagac cgtactggcg
     2341 aacgaacaag ttatcgacgg ctgctgctgg cgctgcgata ccaaagttga acgtaaagag
     2401 atcccgcagt ggtttatcaa aatcactgct tacgctgacg agctgctcaa cgatctggat
     2461 aaactggatc actggccaga caccgttaaa accatgcagc gtaactggat cggtcgttcc
     2521 gaaggcgtgg agatcacctt caacgttaac gactatgaca acatgctgac cgtttacact
     2581 acccgcccgg acacctttat gggttgtacc tacctggcgg tagctgcggg tcatccgctg
     2641 gcgcagaaag cggcggaaaa taatcctgaa ctggcggcct ttattgacga atgccgtaac
     2701 accaaagttg ccgaagctga aatggcgacg atggagaaaa aaggcgtcga tactggcttt
     2761 aaagctgttc acccattaac gggcgaagaa attcccgttt gggcagcaaa cttcgtattg
     2821 atggagtacg gcacgggcgc agttatggcg gttccgggtc acgaccagcg cgactacgag
     2881 tttgcctcta aatacggcct gaacatcaaa ccagttatcc tggctgctga cggctctgag
     2941 ccagatcttt ctcagcaagc cctgactgaa aaaggcgtgc tgttcaactc tggcgaattc
     3001 aacggtcttg accatgaagc ggccttcaac gccatcgccg ataaactgac tgcgatgggc
     3061 gttggcgagc gtaaagtgaa ctaccgcctg cgcgactggg gtgtttctcg tcagcgttac
     3121 tggggcgcgc cgattccgat ggtgacgctg gaagacggta ccgtaatgcc gaccccggac
     3181 gaccagctgc cggtgatcct gccggaagat gtggtgatgg acggcattac cagcccgatt
     3241 aaagcagatc cggagtgggc aaaaactacc gttaacggta tgccagcgct gcgtgaaacc
     3301 gacactttcg acacctttat ggagtcctcc tggtactatg cgcgctacac ttgcccagag
     3361 tacaaagaag gtatgctgga ttccgaagcg gctaactact ggctgccggt ggatatctac
     3421 attggtggta tcgaacacgc cattatgcac ctgctctact tccgcttctt ccacaaactg
     3481 atgcgtgatg caggcatggt gaactctgac gaaccagcga aacagttgct gtgtcagggt
     3541 atggtgctgg cagatgcctt ctactatgtt ggcgaaaacg gcgaacgtaa ctgggtttcc
     3601 ccggttgatg ctatcgttga acgtgacgag aaaggccgta tcgtgaaagc gaaagatgcg
     3661 gcaggccatg aactggttta taccggcatg agcaaaatgt ccaagtcgaa gaacaacggt
     3721 atcgacccgc aggtgatggt tgaacgttac ggcgcggaca ccgttcgtct gtttatgatg
     3781 tttgcttctc cggctgatat gactctcgaa tggcaggaat ccggtgtgga aggggctaac
     3841 cgcttcctga aacgtgtctg gaaactggtt tacgagcaca cagcaaaagg tgatgttgcg
     3901 gcactgaacg ttgatgcgct gactgaagat cagaaagcgc tgcgtcgcga tgtgcataaa
     3961 acgatcgcta aagtgaccga tgatatcggc cgtcgtcaga ccttcaacac cgcaattgcg
     4021 gcgattatgg agctgatgaa caaactggcg aaagcaccaa ccgatggcga gcaggatcgc
     4081 gctctgatgc aggaagcgct gctggccgtt gtccgtatgc ttaacccgtt caccccgcac
     4141 atctgcttca cgctgtggca ggaactgaaa ggcgaaggcg atatcgacaa cgcgccgtgg
     4201 ccggttgctg acgaaaaagc gatggtggaa gactccacgc tggtcgtggt gcaggttaac
     4261 ggtaaagtcc gtgccaaaat caccgttctg gtggacgcaa cggaagaaca ggttcgcgaa
     4321 cgtgctggcc aggaacatct ggtagcaaaa tatcttgatg gcgttaccgt gcgtaaagtg
     4381 atttacgtac caggtaaact cctcaatctg gtcgttggct aagcgcggga ggaagcgtgc
     4441 gatatctggc aacattgttg ttatctctgg cggtgttaat caccgccggg tgtggctggc
     4501 atctgcgtga taccacgcag gttccttcca ctatgaaggt catgatcctg gactcaggcg
     4561 atccgaacgg gccattaagc cgtgcagtgc gtaaccagtt acgtctgaat ggtgtcgagt
     4621 tgcttgataa agaaaccacg cgtaaggacg ttccatccct gcgtttgggt aaagtgagca
     4681 tcgcgaaaga taccgcatcg gtattccgta acggtcaaac agcagagtat cagatgatca
     4741 tgacggtgaa tgcgaccgtg ttgatccccg gccgtgatat ctacccgatt agcgccaaag
     4801 tcttccgttc gttctttgat aacccgcaaa tggcgttagc gaaagataac gaacaagaca
     4861 tgatcgtaaa agagatgtac gaccgtgctg ccgaacagct gattcgtaag ctgccaagca
     4921 tccgtgctgc ggatattcgt tccgacgaag aacagacgtc gaccacaacg gatactccgg
     4981 caacgcctgc acgcgtctcc accacgctgg gtaactgatg attcggttgt acccggaaca
     5041 actccgcgcg cagctcaatg aagggctgcg cgcggcgtat cttttacttg gtaacgatcc
     5101 tctgttattg caggaaagcc aggacgctgt tcgtcaggta gctgcggcac aaggattcga
     5161 agaacaccac actttttcca ttgatcccaa cactgactgg aatgcgatct tttcgttatg
     5221 ccaggctatg agtctgtttg ccagtcgaca aacgctattg ctgttgttac cagaaaacgg
     5281 accgaatgcg gcgatcaatg aacaacttct cacactcacc ggacttctgc atgacgacct
     5341 gctgttgatc gtccgcggta ataaattaag caaagcgcaa gaaaatgccg cctggtttac
     5401 tgctcttgcg aatcgcagcg tgcaggtgac ctgtcagaca ccggagcagg ctcagcttcc
     5461 ccgctgggtt actgcgcgcg caaaacagct caacttagaa ctggatgacg cggcaaatca
     5521 ggtgctctgc tactgttatg aaggtaacct gctggcgctg gctcaggcac tggagcgttt
     5581 atcgctgctc tggccagacg gcaaattgac attaccgcgc gttgaacagg cggtgaatga
     5641 tgccgcgcat ttcacccctt ttcattgggt tgatgctttg ttgatgggaa aaagtaagcg
     5701 cgcattgcat attcttcagc aactgcgtct ggaaggcagc gaaccggtta ttttgttgcg
     5761 cacattacaa cgtgaactgt tgttactggt taacctgaaa cgccagtctg cccatacgcc
     5821 actgcgtgcg ttgtttgata agcatcgggt atggcagaac cgccggggca tgatgggcga
     5881 ggcgttaaat cgcttaagtc agacgcagtt acgtcaggcc gtgcaactcc tgacacgaac
     5941 ggaactcacc ctcaaacaag attacggtca gtcagtgtgg gcagagctga aagggttatc
     6001 tcttctgttg tgccataaac ccctggcgga cgtatttatc gacggttgat atgaaatctt
     6061 tacaggctct gtttggcggc acctttgatc cggtgcacta tggtcatcta aaacccgtgg
     6121 aaacgctggc gaatttgatt ggtctgacgc gggtcacaat catccctaat aatgttcctc
     6181 cgcatcgtcc ccagccggaa gcgaacagcg tgcagcgtaa acacatgctt gaactggcga
     6241 ttgccgacaa gccattattt actcttgatg aacgcgagct aaagcgcaat gccccctctt
     6301 acactgcgca aacactgaaa gagtggcggc aggaacaagg accggacgtg ccgctggcgt
     6361 ttattattgg tcaggattca ctgctgacct ttccgacctg gtacgaatac gaaacgatac
     6421 tcgacaatgc acatttgatc gtctgtcggc gtccaggtta cccacttgaa atggcgcaac
     6481 cgcaatacca gcaatggctg gaagatcatt tgacacataa cccggaagat cttcaccttc
     6541 agcctgccgg taaaatttat ctggctgaaa cgccgtggtt taacatctcg gcgaccatca
     6601 tccgcgaacg tttgcaaaac ggtgaatcgt gtgaggattt attgccggaa ccggtactga
     6661 cttacattaa ccaacaaggc ttgtatcgct gatacctgtc gttacggagg gcatgatgcg
     6721 actgtggtta attcgtcatg gtgaaacgca agcgaatatc gatggtcttt acagcggtca
     6781 tgcgcccacc cccctgaccg cgcgcggtat tgagcaagcg caaaatctgc atacgctgct
     6841 acatggtgtt tcctttgatc tggttttatg cagtgaactg gaacgggcac agcataccgc
     6901 acgactggtt ctcagtgacc gccagctccc cgtgcaaatc atccctgaac tcaacgaaat
     6961 gttttttggc gactgggaga tgcgacatca tcgcgacctc atgcaagaag atgccgaaaa
     7021 ctatagcgcg tggtgcaatg actggcagca tgcaatcccc acgaacggtg aaggatttca
     7081 ggcattttcg caacgtgtgg aacgctttat cgcaaggctt agtgaatttc agcactatca
     7141 gaatatttta gtcgtcagcc atcagggtgt actgagtctg ttaatcgccc gtttaattgg
     7201 catgcctgcc gaggctatgt ggcattttcg cgttgactag gggtgttgga gcgccattga
     7261 tatcaaccaa aaattcgcga cgctacgcgt cctcaatagc cgtgccatcg gggtcgagaa
     7321 tgcatgactt ttctgttttt ttacgggtaa gccgcaacga ccattgacag acccgggcag
     7381 gctgatattc tccgcagcca gacttttttc gccagacacg actttgtaga aattgtttta
     7441 caaaaatggc gatgcaatct gcggcgcggg gtgggatgat agcccacttt cgaaagccga
     7501 ttcggcgaca attgtcccga aatcgcctct ggttcaggta tactgacaga ccatttttat
     7561 ctatttgatt cacccagggg gaaaacttgc agggtaaagc actccaggat tttgttatcg
     7621 acaaaattga tgacctcaaa ggtcaggaca tcatcgcctt agacgttcaa ggcaaatcca
     7681 gcatcaccga ctgcatgatc atctgtacgg gtacgtccag ccgtcatgtt atgtccattg
     7741 ctgaccacgt tgtgcaggag tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg
     7801 aaaacagcgc cgactggatt gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg
     7861 aagagagccg tcgcctgtat gaactggaaa aactctggag ttaatgcgtg aagctgcaac
     7921 ttgtcgccgt gggaacgaaa atgccggact gggtacaaac cggttttacc gagtacctgc
     7981 gtcgttttcc gaaagatatg cccttcgagc tgattgaaat tccggccgga aaacgcggca
     8041 agaatgcgga catcaagcgc atactcgaca aagagggtga gcagatgttg gcggccgcag
     8101 gcaaaaaccg cattgtcacc ctcgatattc caggcaagcc ctgggatacg ccgcagttag
     8161 ccgctgagct ggaacgctgg aagctggatg gtcgcgacgt cagtctactg attggcgggc
     8221 ctgaagggtt gtcgcctgcc tgtaaagcgg cggcggagca gagctggtcg ctgtcggcgc
     8281 ttaccctccc ccatccgctg gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga
     8341 gcatcaccac caaccatcct tatcaccgtg agtgataagg gagctttgag tagaaaacgc
     8401 agcggatgaa actacagaac tcttttcgcg actatacggc agagtccgcg ctgtttgtgc
     8461 gccgggcgct ggtcgccttt ttggggattt tgctgctgac cggcgtgctt atcgccaacc
     8521 tgtataatct gcaaattgtt cgctttaccg actaccagac tcgctctaat gaaaaccgca
     8581 ttaagctggt gcctatcgcg cccagccgcg gcattatcta cgaccgtaat ggtatccctc
     8641 tggccctcaa ccgcactatc taccagatag aaatgatgcc agagaaagtc gataacgtgc
     8701 agcaaacgct ggacgctttg cgcagcgtgg ttgatctgac cgatgacgat attgctgcat
     8761 tccgaaaaga gcgcgcacgt tcacaccgtt tcacctctat tccggtgaaa actaacctga
     8821 ccgaagtaca agtagctcgc tttgccgtca atcagtaccg ttttccgggt gtcgaagtta
     8881 aaggctataa acgtcgttac tatccttacg gttcggcgtt gacccacgtc atcggctatg
     8941 tgtcgaaaat caacgataaa gacgtcgaac gcctgaataa tgacggcaaa ctggccaact
     9001 atgcggcaac gcatgatatc ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc
     9061 acggtcagac cggttatgaa gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt
     9121 taaaagaagt accaccgcaa gccggacacg atatttacct gacgctggat ctcaaactcc
     9181 agcaatatat tgaaacgctg ctggcgggta gccgcgcagc tgtggttgtc accgatccgc
     9241 gtacaggtgg ggtgctggcg ctggtttcca cgcctagtta tgacccaaac ctgtttgttg
     9301 acggtatctc cagcaaagat tattccgcct tgttgaacgc cccgaataca ccgctggtga
     9361 accgcgccac acagggggtt tatcctcccg cgtctacagt taaaccctat gtggcggttt
     9421 cggcattgag cgccggggtg atcacgcgca atacgacgct gtttgaccca ggctggtggc
     9481 aactgccagg ttcggaaaaa cgttatcgtg actggaaaaa atggggccac gggcgtctga
     9541 atgtcacaag atcgctggaa gaatctgcgg ataccttctt ctatcaggtg gcctacgata
     9601 tggggatcga tcgcctctcc gaatggatgg gtaaattcgg ttatggtcat tacaccggta
     9661 tcgacctggc ggaagaacgt tccggcaaca tgcctacccg cgaatggaaa cagaaacgct
     9721 ttaaaaaacc gtggtatcag ggtgacacca ttccggttgg tatcggtcag ggttactgga
     9781 cagcgacccc aatccagatg agtaaggcac tgatgatcct gattaatgac ggtatcgtga
     9841 aggttcctca tttgctgatg agcaccgccg aagacggcaa acaggtgcca tgggtacagc
     9901 cgcatgaacc gcccgtcggc gatattcatt ccggttactg ggagctggcg aaagacggta
     9961 tgtacggtgt tgctaaccgc cctaacggta cggcgcataa atactttgcc agcgcaccgt
    10021 acaaaattgc ggcgaaatcc ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct
    10081 ataatgcgca caaaattgcc gagcgtttac gtgaccacaa actgatgacc gcctttgcgc
    10141 catacaacaa tccgcaagtg gctgtcgcca tgattctgga gaacggtggt gcgggtccgg
    10201 cggttggtac actgatgcgc cagatcctcg accacattat gctgggtgat aacaacaccg
    10261 atctgcctgc ggaaaatcca gcggttgccg cagcggagga ccattaatca tgacggataa
    10321 tccgaataaa aaaacattct gggataaagt ccatctcgat cccacaatgc tgctgatctt
    10381 actggcattg ctggtttaca gcgccctggt tatctggagc gccagcggtc aggatattgg
    10441 catgatggag cgtaaaatcg gccaaatcgc gatgggtcta gtcatcatgg tggtgatggc
    10501 gcaaattcct ccacgcgttt atgaaggctg ggccccctat ctctatatca tctgtattat
    10561 tttgctggtg gcggtagatg ctttcggtgc catctctaaa ggtgctcaac gctggctgga
    10621 cctcggtatt gttcgttttc agccgtcgga aattgccaaa atagccgtac cactgatggt
    10681 tgcgcgcttt atcaaccgcg acgtttgccc gccatcgttg aagaacactg gcatcgcgct
    10741 ggtgctgata tttatgccca cgctgctggt ggctgcacag cctgacctgg gaacatcaat
    10801 cctcgttgcg ctttccggtc tgtttgtact gttcctctct ggccttagct ggcgtctgat
    10861 tggcgtcgca gtagtgctgg tggcggcgtt cattccgatt ctgtggttct tcctgatgca
    10921 tgattaccag cgccagcgcg taatgatgct cctagacccg gaatcagacc cactcggcgc
    10981 gggctatcac attattcagt ctaaaattgc tattggctcc ggcggattac gcggcaaagg
    11041 ctggctgcac ggcactcagt cacagcttga atttctcccc gaacgccata ccgactttat
    11101 cttcgcggta ctggcggaag agctgggatt agtgggcatt ctgattctgc tcgctctcta
    11161 cattctgctg atcatgcgcg ggctgtggat agccgccaga gcgcaaacca cctttggtcg
    11221 cgtcatggct ggtggcttaa tgctgatatt attcgtttat gtcttcgtaa atattggtat
    11281 ggtaagcggt attctgccgg ttgtaggggt tccgctccca ctggtcagtt atggaggatc
    11341 ggcgctaatt gtgctgatgg ctgggttcgg gattgtaatg tcaatccaca cccacaggaa
    11401 aatgttgtcg aaaagcgtgt aagaggtgcg caatgcgtaa gcagtggctc gggatctgca
    11461 tcgcggcagg aatgctcgcg gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg
    11521 taccgcagcc tgcggtatgt aacggcccca tagttgaaat tagcggggcg gacccgcgtt
    11581 tcgaaccact gaacgcgacg gcaaatcagg attaccagcg cgacggtaaa agctacaaaa
    11641 tcgtgcagga tccgtctcga tttagccagg cgggactggc ggcaatctat gatgccgaac
    11701 ccggcagtaa cctgacggcc tctggcgaag ctttcgatcc gacacagctg acggcggccc
    11761 atccaacgct tccgatcccc agctacgcca gaatcactaa cctggctaac gggcgaatga
    11821 tcgtggtgcg cattaatgat cgcggccctt acggcaacga ccgcgttatt tcactttctc
    11881 gcgcggcagc tgaccgtctt aacacgtcaa acaacaccaa agttcgtatc gatccgatta
    11941 ttgtcgccca ggatggttcg ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac
    12001 agacttacgc cctgccagca cctcccgatt taagcggtgg cgcgggaaca agttcagtgt
    12061 ctggcccgca gggtgacatt cttccggtca gtaattcgac gctaaaaagc gaagatccga
    12121 ccggcgcgcc ggtaaccagt agcggtttcc tcggcgctcc aacgacctta gcgcctggtg
    12181 tactggaagg cagcgaaccg acgcctgcgc cacagcccgt tgttacagct ccgtcgacaa
    12241 cgcctgcaac ctcgcctgca atggtgacac cgcaagccgc ctcgcaaagc gccagcggca
    12301 actttatggt gcaagtcggg gccgtaagcg atcaggctcg tgcgcaacag taccaacagc
    12361 aactgggaca gaagttcggc gtccccggtc gcgtaactca aaatggcgcg gtctggcgga
    12421 tccagcttgg cccattcgcc agcaaagccg aagccagtac cttgcagcaa cgtttgcaaa
    12481 ccgaagccca attacagtca tttattacta ccgcgcagta gcgtaaagca ggcatctgat
    12541 gtgtcaattt ctgtaaatga cgttaacaaa gtcacgcgga aagtcagatg cctgccggta
    12601 gtgcatttgc tatagtaggg cactttttta attccatcac ggatgtcgta gttcagacca
    12661 tgaataccat tttttccgct cgtatcatga agcgcctggc gctcaccacg gctctttgca
    12721 cagcctttat ctctgctgca catgccgatg acctgaatat caaaactatg atcccgggtg
    12781 taccgcagat cgatgcggag tcctacatcc tgattgacta taactccggc aaagtgctcg
    12841 ccgaacagaa cgcagatgtc cgccgcgatc ctgccagcct gaccaaaatg atgaccagtt
    12901 acgttatcgg ccaggcaatg aaagccggta aatttaaaga aactgattta gtcactatcg
    12961 gcaacgacgc atgggccacc ggtaacccgg tgtttaaagg ttcttcgctg atgttcctca
    13021 aaccgggcat gcaggttccg gtttctcagc tgatccgcgg tattaacctg caatcgggta
    13081 acgatgcttg tgtcgccatg gccgattttg ccgctggtag ccaggacgct tttgttggct
    13141 tgatgaacag ctacgttaac gcactgggcc tgaaaaatac ccacttccag acggtacatg
    13201 gtctggatgc tgatggtcag tacagctccg cgcgagatat ggcgctgatc ggccaggcat
    13261 tgatccgtga cgtaccgaat gaatactcga tctataaaga aaaagaattt acgtttaacg
    13321 gtattcgcca gctgaaccgt aacggcctgt tatgggataa cagcctgaat gtcgacggca
    13381 tcaaaaccgg acacactgac aaagcaggtt acaaccttgt tgcttctgcg actgaaggcc
    13441 agatgcgctt gatttctgcg gtgatgggcg gacgtacttt taaaggccgt gaagccgaaa
    13501 gtaaaaaact gctaacctgg ggcttccgtt tcttcgaaac cgttaaccca ctgaaagtag
    13561 gtaaagagtt cgcctctgaa ccggtttggt ttggtgattc tgatcgcgct tcgttagggg
    13621 ttgataaaga cgtgtacctg accattccac gtggtcgcat gaaagatctg aaagccagct
    13681 atgtgctgaa cagcagtgaa ttgcatgcgc cgctgcaaaa gaatcaggtc gtcggaacta
    13741 tcaacttcca gcttgatggc aaaacgatcg agcaacgccc gctggttgtg ttgcaagaaa
    13801 tcccggaagg taacttcttc ggcaaaatca ttgattacat taaattaatg ttccatcact
    13861 ggtttggtta aaaattgaac acttgaaagt gtaatttccg tccccatata ctaagcatca
    13921 gtaaaaaact cccgccttct ggcgggagtt gctatttaat tacgttacgc cggagctgac
    13981 atgaaaacca aacttaacga actgcttgaa ttccctactc cttttactta caaagttatg
    14041 gggcaggcgt tacctgagct ggttgatcag gtggttgaag tggtacagcg ccatgcgcca
    14101 ggtgactaca ccccaacggt aaaaccaagc agcaaaggca actaccactc ggtatctatc
    14161 actatcaacg ccactcatat cgagcaggtt gaaacactgt atgaagaact aggcaaaatc
    14221 gatattgtcc gcatggttct gtaactcgct tctccgttac ccggcccctg gtcgggtaac
    14281 tcccctctcc tcgctgtgat atacttttcc cccactttta ctcattctcc acggagatgc
    14341 agttttgtat caggataaaa ttcttgtccg ccagctcggt cttcagcctt acgagccaat
    14401 ctcccaggct atgcatgaat tcaccgatac ccgcgatgat agtacccttg atgaaatctg
    14461 gctggtcgag cactatccgg tattcaccca aggtcaggca ggaaaagcgg agcacatttt
    14521 aatgccgggt gatattccgg tgatccagag cgatcgcggt gggcaggtga cttatcacgg
    14581 gccggggcaa caggtgatgt atgtgttgct taacctgaaa cgccgtaaac tcggtgtgcg
    14641 tgaactggtg accttgcttg agcaaacagt ggtgaatacc ctggctgaac tgggtataga
    14701 agcgcatcct cgggctgacg cgcctggtgt ctatgtcggg gaaaagaaaa tttgctcact
    14761 gggtttacgt attcgacgcg gttgttcatt ccacggtctg gcattaaacg tcaatatgga
    14821 tctttcacca tttttacgta ttaatccttg tgggtatgcc ggaatggaaa tggctaaaat
    14881 atcacaatgg aaacccgaag cgacgactaa taatattgca ccacgtttac tggaaaatat
    14941 tttagcgcta ctaaacaatc ctgacttcga atatattacc gcttaattgc atacctcaat
    15001 ggcccaattt acactgggtc attactcata atacctgcta atgctttaca tctccttcgc
    15061 gtttgtatac tttctttatc acttgcagaa atgcggtgct acgccttgct ccgtaactca
    15121 ccccattgat taacctattg aaatagttga ttatggtgag caaaagcgtt ccgttccaat
    15181 tagacataag attattgtaa tttttttaaa tattattttt ccatgaataa aattggagta
    15241 agtgcgtgga tagtaataat caaattgaac cctgtttaag cagaaagtcg tcagaaggta
    15301 aaccacaaat atttacaacc ttacgaaata ttgatcttaa ccttctgact atttttgaag
    15361 ctgtatatgt acataaaggg atcgttaatg cagcgaaagt gcttaatctg accccctcgg
    15421 caatcagtca gtctattcag aaactgcgcg ttatattccc tgacccattg tttattcgca
    15481 aaggccaggg tgtcactcct accgcatttg cgatgcatct acatgagtat atcagtcagg
    15541 gccttgagtc cattcttggc gcgctggata tcgaaggaag ctatgataag caacgaacga
    15601 taactattgc gaccactccc tcggtcggag ccctggtcct tcctgtcatc tatcgggcga
    15661 ttaaaactca ctatccgcag cttttactgc gcaacccgcc catcagcgac gcggaaaacc
    15721 aactcagtca gtttcaaacc gatctcatca tcgataacat gttttgcacc aatcgtacgg
    15781 tgcaacatca tgttctgttc accgacaata tggtgttaat ttgccgcgag ggaaatccac
    15841 tactctcttt agaagatgac agagagacta tcgacaacgc tgcgcatgta ctcctgttac
    15901 cggaagggca aaatttcagc ggtctgcggc agagagttca agagatgttt ccggaccggc
    15961 aaatcaattt caccagctac aacattttga caatcgctgc actggttgcc aacagtgaca
    16021 tgttagcgat tattcccagc cgtttttata acctgtttag tcgttgctgg ccgctggaaa
    16081 aactgccttt tccgtcctta aatgaggagc aaatagattt ctctatccac tacaacaaat
    16141 tcagcctgcg tgatcccatc ctgcacgggg taatagatgt tatccgtaat gcattttgaa
    16201 aaagtattgt tacagccaga tcacagcgca gaacgataaa atggcacaat ccaacaacaa
    16261 ttttacattt tcgcgaccgc tttggctgct ttcaggtccg tttcaatgat atactgccag
    16321 tcgttaattc aaaaatagtt gataattaca acaatctatt gaattgaaac gctttccttc
    16381 gtaattcgca actggaacac gcacgctatg agtaaaccca ttgtgatgga acgcggtgtt
    16441 aaataccgcg atgccgataa gatggccctt atcccggtta aaaacgtggc aacagagcgc
    16501 gaagccctgc tgcgcaagcc ggaatggatg aaaatcaagc ttccagcgga ctctacacgt
    16561 atccagggca tcaaagccgc aatgcgcaaa aatggcctgc attctgtctg cgaggaagcc
    16621 tcctgcccta acctggcgga atgcttcaac cacggcacag caacgtttat gatcctcggc
    16681 gctatttgta cccgccgttg tccgttctgt gatgttgccc acggtcgccc ggtagctcct
    16741 gatgccaatg aaccagtgaa actggcgcag accattgccg atatggcgct gcgttatgtg
    16801 gttatcacct ccgttgaccg tgatgacctg cgcgatggcg gtgcccagca ctttgcggat
    16861 tgcattactg ccattcggga aaaaagcccg caaatcaaaa ttgaaactct ggtgccggat
    16921 ttccgtggtc gtatggatcg tgctctggat attctgactg caacgccacc agatgtgttc
    16981 aaccataacc tggaaaacgt accgcgtatt taccgtcagg tacggcctgg tgcagattac
    17041 aactggtcgc tgaagctgct ggaacgcttt aaagaagcgc atccggaaat cccgaccaag
    17101 tctggtctga tggtgggact gggtgaaacc aatgaagaaa ttattgaggt aatgcgcgac
    17161 ctgcgccgtc atggtgtgac gatgttaacg ctggggcaat atttgcagcc aagccgccat
    17221 cacctgccgg ttcaacgtta cgttagcccg gatgagttcg acgaaatgaa agccgaagcg
    17281 ctggcgatgg gctttaccca tgctgcatgc ggtccgtttg tccgctcttc ttaccacgcc
    17341 gatttgcagg cgaaagggat ggaagttaag taatcctgta ataattactt acatctgtct
    17401 gataaaaaac ccgctttttg
//
LOCUS       DAZPAC010000075        17390 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZPAC010000075 DAZPAC010000000
VERSION     DAZPAC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17390
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.075"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            230..1663
                     /gene="cscA"
                     /locus_tag="ACHUPM_002106"
     CDS             230..1663
                     /gene="cscA"
                     /locus_tag="ACHUPM_002106"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR0869883.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1671..2666)
                     /locus_tag="ACHUPM_002107"
     CDS             complement(1671..2666)
                     /locus_tag="ACHUPM_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0869884.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2897..3115
                     /locus_tag="ACHUPM_002108"
                     /pseudo
     CDS             2897..3115
                     /locus_tag="ACHUPM_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3133..4461
                     /gene="dsdA"
                     /locus_tag="ACHUPM_002109"
     CDS             3133..4461
                     /gene="dsdA"
                     /locus_tag="ACHUPM_002109"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR0869885.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="ACHUPM_002110"
     CDS             complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="ACHUPM_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR0869886.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6107..7270)
                     /gene="emrK"
                     /locus_tag="ACHUPM_002111"
     CDS             complement(6107..7270)
                     /gene="emrK"
                     /locus_tag="ACHUPM_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR0869887.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7686..8300
                     /gene="evgA"
                     /locus_tag="ACHUPM_002112"
     CDS             7686..8300
                     /gene="evgA"
                     /locus_tag="ACHUPM_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR0869888.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8305..11898
                     /gene="evgS"
                     /locus_tag="ACHUPM_002113"
     CDS             8305..11898
                     /gene="evgS"
                     /locus_tag="ACHUPM_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR0869889.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11954..13099)
                     /gene="yfdE"
                     /locus_tag="ACHUPM_002114"
     CDS             complement(11954..13099)
                     /gene="yfdE"
                     /locus_tag="ACHUPM_002114"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR0869890.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="ACHUPM_002115"
     CDS             complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="ACHUPM_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR0869891.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="ACHUPM_002116"
     CDS             complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="ACHUPM_002116"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR0869892.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15935..17185)
                     /gene="frc"
                     /locus_tag="ACHUPM_002117"
     CDS             complement(15935..17185)
                     /gene="frc"
                     /locus_tag="ACHUPM_002117"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR0869893.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         5016 a         4010 c         3741 g         4623 t
ORIGIN      
        1 actttggctg acattttttt cccttaaatt catctgagtt acgcatagtg ataaacctct
       61 ttctcgcaaa atcgtcatgg atttactaaa acatgcatat tcgatcacaa aacgtcatag
      121 ttaacgttaa catttgtgat attcatcgca tttatgaaag taagggacta tatttttata
      181 aaagttaacg ttaacaattc accaaatttg cttaaccagg atgattaaaa tgacgcaatc
      241 tcgattgcat gcggcgcaaa acgcactagc aaaacttcat gagcaccggg gtaacacttt
      301 ctatccccat tttcacctcg cgccacctgc cggatggatg aacgatccaa acggcctgat
      361 ctggtttaac gatcgttatc acgcgtttta tcaacatcac ccgatgagcg aacactgggg
      421 accaatgcac tggggacatg ccaccagcga cgatatgatc cactggcagc atgagcctat
      481 tgcgctagcg ccaggagacg ataatgacaa agacgggtgt ttttcaggta gtgctgtcga
      541 tgacaatggt gtcctctcac ttatctacac cggacacgtc tggctcgatt gtgcaggtaa
      601 tgacgatgca attcgcgaag tacaatgtct ggctaccagt cgggatggta ttcatttcga
      661 gaaacagggt gtgatcctca ctccaccaga aggaatcatg cacttccgcg atcctaaagt
      721 gtggcgtgaa gccgacacat ggtggatggt agtcggggcg aaagatccag gcaacacggg
      781 gcagatcctg ctttatcgcg gcagttcatt gcgtgaatgg accttcgatc gcgtactggc
      841 ccacgctgat gcgggtgaaa gctatatgtg ggaatgtccg gactttttca gccttggcga
      901 tcagcattat ctgatgtttt ccccgcaggg aatgaatgcc gagggataca gttaccgaaa
      961 tcgctttcaa agtggcgtaa tacccggaat gtggtcgcca ggacgacttt ttgcacaatc
     1021 cgggcatttt actgaacttg ataacgggca tgacttttat gcaccacaaa gctttttagc
     1081 gaaggatggt cggcgtattg ttatcggctg gatggatatg tgggaatcgc caatgccctc
     1141 aaaacgtgaa ggatgggcag gctgcatgac gctggcgcgc gagctatcag agagcaatgg
     1201 caaacttcta caacgcccgg ttcacgaagc tgagtcgtta cgccagcagc atcaatctgt
     1261 ctctccccgc acaatcagca ataaatatgt tttgcaggaa aacgcgcaag cagttgagat
     1321 tcagttgcag tgggcgctga agaacagtga tgccgaacat tacggattac agctcggcac
     1381 tggaatgcgg ctgtatattg ataaccaatc tgagcgactt gttttgtggc ggtattaccc
     1441 acacgagaat ttagacggct accgtagtat tcccctcccg cagcgtgaca cgctcgccct
     1501 aaggatattt atcgatacat catccgtgga agtatttatt aacgacgggg aagcggtgat
     1561 gagtagtcga atctatccgc agccagaaga acgggaactg tcgctttatg cctcccacgg
     1621 agtggctgtg ctgcaacatg gagcactctg gctactgggt taacataata tcaggtggaa
     1681 caacggatca acagcggaca agggatccgc gtcactcttc ccccttcacg accttcaata
     1741 atatgcaatg cagcttcccg cccgataatg tcatgtggaa gctgaattgt ggtcagcggc
     1801 ggtaaaaaca gatgcccgac gccaaccaga ttatcaaagc ccattacggc gacatcctgc
     1861 gggatacgta cccccttcgc caggagaacc tgataagcga caaaggctgc acgatcgtta
     1921 ccacatatca gaacatcaaa atctggtttg cccgatttga agtgggcatt gagtaaactt
     1981 gcgagatcgg tgtagtgatc atcacctgtt gccatgtgaa attgtttcac ctcagccaga
     2041 tctcgtccag catcacgcca ggcctgctca aatccctgcc gacgataccc tgttgccaac
     2101 gcactttccg gtagccagaa gcataacggt tgacgatagc ccgccgcgag caaatgctgt
     2161 gttgattcat attgtgcagt gtaatcatca gggatataac tgggtaacgc tgggtcatcc
     2221 gccacacagt tcgccaatac aatattttca ccatacagag actcaggcag cgtgatatgt
     2281 cgcagcccca ttgtagtata gataatgcca tccggacggt gggcaagcag ctgacgtgcc
     2341 gcgcgggcag cgtcatcttc agaaaaaata ttgattaaaa aactattcca gccgaactcg
     2401 ctggcggttt gctcaatggc aagcagaata tcaacagaga aaggagtggt agccgtgtcc
     2461 tgcgccagca cggcgagagt cgacggctta cgtccttgag cgcgcatctt acgggcggaa
     2521 agatcaggaa cataattcag ggtctggatt gcctgcaata cgcggtcacg cgttgcagga
     2581 cgcacagatt ctgcattatg catcacccgg gagactgtca tcatcgacac tcccgccagg
     2641 cgtgcgacat cctttaatga agccataccc aagccgtttg ccgtaaaacg ggcactgtag
     2701 cagaaacaga cgtcactggc gagatccaac gccctatcac ctgacacagc aatacaataa
     2761 aaaataacaa taattcccgg acaattgtcc ccaattccgc ctctgttctc gccaacgagt
     2821 aaccatatag gcaagtaagt aaaacatgcc aactggcatc gccaacctgg agaagaaaat
     2881 ataaatatgg atcattgtgc tgccgctgta tcccgacatc agcccggaaa ttattgcgat
     2941 tgctatcggt tcaggtgcaa ttggctgcac gatcgttacg gactcgcttt tctggctagt
     3001 gaagcaatat tgcggcgcta cgctcaatga aacatttaaa tactatacga cagcgacatt
     3061 tatcgcttca gtcgtcgctc tggcgggcac attcctgctg tcatttatca tctaagcgca
     3121 aagagacgta ctatggaaaa cgctaaaatg aactcgctca tcgcccagta tccgttggta
     3181 aaggatctgg ttgctcttaa agaaaccacc tggtttaatc ctggcacgac ctcattggct
     3241 gaaggtttac cttatgttgg cctgaccgaa caggatgttc aggacgccca tgcgcgctta
     3301 tcccgttttg caccctatct ggcaaaagca tttgctgaaa ctgctgccac tggggggatt
     3361 attgaatcag aactggttgc cattccagct atgcaaaaac ggctggaaaa agaatatcag
     3421 caaccgatca gcgggcaact gttactgaaa aaagatagcc atttgcccat ttccggctcc
     3481 ataaaagcac gcggcgggat ttatgaagtc ctggcacacg cagaaaaact ggctctggaa
     3541 gcggggttgc tgacgcttga tgatgactac agcaaactgc tttctccgga gtttaaacag
     3601 ttctttagcc aatacagcat tgctgtgggc tcaaccggaa atctggggtt atcaatcggc
     3661 attatgagcg cccgcattgg ctttaaggtg acagttcata tgtctgctga tgcccgggca
     3721 tggaaaaaag cgaaactgcg cagccatggc gttacggtcg tggaatatga gcaagattat
     3781 ggtgttgccg tcgaggaagg acgtaaagca gcgcagtctg acccgaactg tttctttatt
     3841 gatgacgaaa attcccgcac gttgttcctt gggtattccg tcgctggcca gcgtcttaaa
     3901 gcgcaatttg cccagcaagg ccgtatcgtc gatgctgata accctctgtt tgtctatctg
     3961 ccgtgtggtg ttggcggtgg tcctggtggc gtcgcattcg ggcttaaact ggcgtttggc
     4021 gatcatgttc actgcttttt tgccgaacca acgcactccc cttgtatgtt gttaggcgtc
     4081 catacaggat tacacgatca gatttctgtt caggatattg gtatcgacaa ccttaccgca
     4141 gcggatggcc ttgcagttgg tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg
     4201 ctggatggct tctataccct tagcgatcaa accatgtatg acatgcttgg ctggctggcg
     4261 caggaagaag gtattcgtct tgaaccttcg gcactggcgg gtatggccgg acctcagcgc
     4321 gtatgtgcat cagtaagtta ccaacagatg cacggtttca gcgcagaaca actgcgtaat
     4381 accactcatc tggtgtgggc gacgggaggt ggaatggtgc cggaagaaga gatgaatcaa
     4441 tatctggcaa aaggccgtta ataacgtttc aacgcagcat cgcaatcctt tccctgggtg
     4501 agcgatgctg ccgatggcgc agacttaaga tccccggtct tacccgctat aaccccctcc
     4561 ttttatcatc acccaacgcc tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc
     4621 gtaagtaaga taaaagcaat agccgccata cggaaaatct catttgcaga aatagaaagt
     4681 gactgctggg ttatttcatt attaatttca ttaagaactc ctgaaagcga accataatat
     4741 ttatccataa tttgtgacga tgaattaaac acggggttaa attgatcgat ggttgctgtc
     4801 aactgactat ggtgtaacga ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca
     4861 actgatcctg acaaggtacg aaaaaaatta ctcatactcg aggcattggc aaatttatta
     4921 tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat
     4981 ccctgaaaaa actgcggcaa aatgatgcct gtaaaatcaa tcgttggcat aaatgtcaca
     5041 gaacgccagt aatagcaaac cgcatacatc aaaaaactaa atgtcactaa caaccgcatg
     5101 tctattttgt tgccataacg tcctatcaaa ggtgaaatta atagtggcat gatgccgatg
     5161 ggcgcataag caagtccggc ccatatcgca ttatacccca tcgtttcctg gagtaactgc
     5221 ggcataagga cgatcgctcc agagtaaaat aaatacgcgc atgtgatact cacaatacca
     5281 atggtgaagt tacgggactt aaacaaactg agatcaagaa tcgggttctc tgaggtcgac
     5341 tcccaaatga ctaaagagat cagagaaata actgatacta ctgttaatat tattattgta
     5401 ctcgagttga accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca
     5461 ccgagcacta acagggtcag tcctggtaga ttcattttga ccggtgaagt ctcagtttct
     5521 cttcctttaa gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt
     5581 aaaaatatcc aaccccagct aaagttatca caaatataac cgcccaatat cggcccacat
     5641 atcggagcga taatcacggt cattgaccat aatgccagag caaatgttct tttttctggc
     5701 ggataattcc ttaataataa actctgtgac agtggaatta acggccccgc cattaacccc
     5761 tgaacgactc taaaaaatat cagcacatca agattggtcg ataggctaca cattaatgaa
     5821 gacagcgaaa aaaaggttac tgaaagtaaa aataatctta attcgcctat tctttgtgcc
     5881 aacctgccag taacagggat cgcaatggca tttgctacac caaacgaggt gataacccag
     5941 gtgccttcgt ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa
     6001 atagtggaat ccaacatttg cataaatgtc gctaatgaca atgcaatagt gacgcaccat
     6061 aacgtcccac cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat
     6121 tatgcgaaat aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct
     6181 tactggtata agccggcatg gaggtcacgg ttgaagccag ctcaggcatc tcagcaatgt
     6241 cttcgtcctt cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca
     6301 tgagttcttt tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt
     6361 tccctgtcgc attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc
     6421 ctgtcacccg accatgaaac acaacatttt caccataaag atcgctgata atattgactg
     6481 attgaccaat ccgtacatcc gtgagttgtg tttctttaaa gttggcatta acccacattt
     6541 gacgtgccgg tacgacagcc attaacgatt gtccgggact cactgtttcg ccgacctgaa
     6601 cacttcgctg ggcaatatag ccagtaaccg gactccttat atccgtacgt ttaagcgcca
     6661 accaggcttc tttggttgca tccgccgctt caactacttg tggctgacgg tttaatggtg
     6721 tgttcattac taaagcttta ttcgctttat aagcctggat agcggcattc agtgccgctt
     6781 tgctacttat taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct
     6841 tcgctaaagg cacacgacgg ttataatctt ctaaggattg ttgatactga atacgtgctg
     6901 aagcgacttc ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa
     6961 tatttgccag attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg
     7021 aaactaaaat gtcaccctgt cgaacgtagt tcgtatcttt atgattaact acagtgacac
     7081 tacctgagac ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac
     7141 taatcatgtc ttctaattcc attgaccaat aggcataggc acctgaaaac gcaataaata
     7201 aaactatcac caataaagaa aagtattttt ttctgttaga ctgtttttta tttgaattaa
     7261 tctgttccac tattatctct catttcgcat agatgaaatt tatgaattga atgaaaagag
     7321 tattcattct tcatatcaaa cacagtgcat gtgttgcaca ttatcccagg aacgttggag
     7381 gcctaattta aatgaggtgc aaaaaaaaca acagaggact aaaccgtggc ttttgcaata
     7441 caattcttac gcctgtagga ttagtgagaa gacttatagt gctaacttga aactataaat
     7501 catcggtata atccctgatt ttattgttga catttcattt atgccgacta tttatatggt
     7561 atacttgtcg aattatctta aaggaagctc agattttctt atttttattg agaaaatgag
     7621 atgacgcctt atgtctgtat tactacaggg agaagggaga tgcttcattg caaagggaat
     7681 aatctatgaa cgcaataatt attgatgacc atcctcttgc tatcgcagca attcgtaatt
     7741 tattgatcaa aaacgatatt gaaatcttag cggagttaac tgaaggcgga agcgccgttc
     7801 agcgggtgga aacacttaag cctgatatcg tcatcattga tgtcgatatc cccggagtta
     7861 acggtatcct ggtgttagaa acgctgagga agcgccaata tagcggaatt attattatcg
     7921 tctccgctaa aaatgaccat ttttacggga aacattgtgc tgatgctggc gctaatggtt
     7981 tcgtgagtaa aaaagaaggc atgaacaata tcattgcggc tattgaagct gcaaaaaatg
     8041 gctactgcta tttccccttc tctctcaacc ggtttgttgg aagtttaacg tccgaccagc
     8101 aaaaactcga ctccttatcg aaacaagaaa ttagtgtcat gcggtatatt cttgatggca
     8161 aggataataa tgacattgcg gaaaaaatgt tcatcagcaa caaaactgtc agcacttata
     8221 aaagtcgcct gatggaaaaa ttagaatgta aatcactgat ggatctttac acattcgcac
     8281 aacgtaacaa aatcggctaa ccacatgaag tttttaccct atatttttct tctctgttgt
     8341 ggtctttggt cgaccataag tttcgcagac gaagattaca tcgaatatcg tggcatcagt
     8401 agtaacaacc gtgtcatact tgatccagta cgtctgagca acaaggaatt acgttggtta
     8461 gcgagcaaaa aaaatcttgt gattgcagta cataagtccc aaacggctac gttgttgcat
     8521 accgattcgc agcaacgagt tcgtggtatt aatgctgatt atttaaatct tttaaaaaga
     8581 acgttaaata tcaaattaac actccgggaa tacgccgatc atcaaaaagc aatggacgcg
     8641 ctggaagaag gtgaagtcga tatagtgtta tcacatttag tagcttcgcc gcctcttaat
     8701 gatgacattg ctgcaaccaa accactgata attacctttc cggcgctggt aaccaccctt
     8761 cacgattcaa tgcgaccgct tacctcatca aaaccagtaa atattgctcg agtagcaaat
     8821 tatccccctg acgaggtaat tcatcaatca tttccaaaag caacaattat ctcttttaca
     8881 aatttatatc aggcattagc atccgtctca gccggacaga atgattactt tattggtagt
     8941 aacatcatta ccagcagtat gatttcccgc tatttcactc actccttaaa tgtagtgaaa
     9001 tattataact caccgcgtca atataatttt ttcttgacca gaaaagaatc tgtcgttctt
     9061 aatgaagtac tcaatcgatt tgttgatgct ttgacaaatg aagttcgcta tgaagtatca
     9121 caaaattggc ttgatacagg aaacctggcc tttctgaaca aaccattaga actcactgaa
     9181 catgaaaaac aatggattaa gcagcatccc gatttaaagg tgctggaaaa tccttactcc
     9241 cctccctatt ctatgacgga tgaaaatggc tcggttcggg gcgttatggg ggatattctt
     9301 aatattatta ctttgcaaac aggtttaaat ttttctccga tcaccgtttc acacaatatc
     9361 catgctggaa cacagctgaa tcccggcgga tgggatatat tacccggtgc tatttatagt
     9421 gaagatagag aaaataatgt tttatttgct gaagccttca taacaacgcc ttacgttttt
     9481 gtcatgcaaa aagcgcctga cagtgaacaa acattaaaaa aaggaatgaa agttgccatt
     9541 ccatattatt atgagcttca ttcgcaatta aaagagatgt atccggaggt agagtggata
     9601 aaagtcgata acgccagcgc tgcatttcac aaggtcaagg aaggcgaact tgatgctctg
     9661 gtcgcgacac agttaaattc acgttacatg atcgaccatt actatcctaa tgaactttat
     9721 cattttctta ttcccggcgt tccgaatgca tcgctttcgt tcgcttttcc tcgcggagaa
     9781 ccggaactta aggatattat taataaagca ctgaatgcaa tccccccaag cgaagttctg
     9841 cgcctgaccg aaaaatggat taaaatgccc aatgtgacca ttgacacatg ggacctctat
     9901 agcgagcaat tttatattgt tacgacatta tccgttttat tagttggcag tagcctttta
     9961 tggggatttt acctgttacg ctcagttcgt cgtcgtaaag tcattcaggg tgatttagaa
    10021 aaccaaatat cattccgaaa agcactctcg gattccttac cgaatccaac ttatgttgta
    10081 aactggcaag gtaatgtcat tagtcataat agtgcttttg aacattattt cactgcggat
    10141 tactacaaaa atgcaatgtt accattagaa aacagtgact ccccctttaa agatgttttt
    10201 tctaatgcgc atgaagtcac agcagaaacg aaagaaaatc gaacaatata cacacaggtt
    10261 tttgaaattg ataatggcat cgagaaaaga tgcattaatc actggcatac attatgcaat
    10321 ctgccagcca gcgatcatgc tgtttatatt tgtggttggc aagatattac agagacgcgt
    10381 gatttaattc atgcactcga agtagagaga aataaagcga tcaatgcaac tgtcgcaaaa
    10441 agtcagtttc tggcaacaat gagtcacgaa ataagaacac caataagctc cattatgggc
    10501 ttcctggaac ttctgtcggg ttctggtctt agcaaggagc aacgggtgga ggcgatttca
    10561 cttgcctact ccaccggaca atcactcctc ggcttaattg gtgaaatcct tgatgtcgac
    10621 aaaattgaat cgggtaacta tcaacttcaa ccacaatggg tcgatatccc tactttagtc
    10681 cagaacactt gtcactcttt cggtgcgatt gctgcaagca aatcgatcgc attaatttgc
    10741 agcagtacat ttcctgatca ttacctggtt aagatcgacc ctcaggcgtt taagcaggtc
    10801 ttatcaaatt tactgagcaa tgctctcaaa tttaccaccg agggggcagt aaaaattacg
    10861 acctccctgg ttcacattga tgacaaccac gctgttatca aaatgacgat tatggattct
    10921 ggaagtggat tatcgcagga agaacaacaa caactcttta aacgctacag ccaaacaagt
    10981 gcaggtcgcc agcaaacagg atctggctta ggcttaatga tctgcaaaga attaattaaa
    11041 aatatgcagg gcgatttgtc attagaaagt catccaggca taggaacaac atttacgatc
    11101 acaatcccgg tagaaattag ccagcaagtg gcgactgtcg aggcaaaagc agaacacccc
    11161 atcacactac ctgaaaagtt gagcatatta atcgcggatg atcatccgac caacaggcta
    11221 ttactcaaac gccagctaaa tctattagga tatgatgttg atgaagccac tgatggtgtg
    11281 caagcgctac acaaagtcag tatgcaacat tatgatctgc ttattactga cgttaatatg
    11341 ccgaatatgg atggttttga gttgactcgc aaactccgtg agcaaaattc ttccttaccc
    11401 atctgggggc ttacagccaa cgcacaggct aacgaacgtg aaaaagggtt aagttgcggc
    11461 atgaacttat gtttgttcaa accgttgacc ctggatgtac tgaaaacaca tttaagtcag
    11521 ttacaccagg ttgcgcatat tgcacctcag tatcgccacc ttgatatcga agccctgaaa
    11581 aataatacgg cgaacgatct acaactgatg caggagattc tcatgacttt ccagcatgaa
    11641 acgcataaag atctacccgc tgcgtttcaa gcactagaag ctggcgataa cagaactttc
    11701 catcagtgta ttcatcgcat ccacggtgcg gctaacatcc tgaatttgca aaagttgatt
    11761 aatattagcc atcagttaga aataacacct gtttcagatg acagtaagcc tgaaattctt
    11821 cagttgctga actctgtaaa agaacacatt gcagagctgg accaggagat tgctgttttc
    11881 tgtcagaaaa atgactaaat agcggctccc acaatgttct aatgtgggag ctatttacca
    11941 gcacatctta catttatgat gagaactcct ggcgaatttg ttccccatgc tggtcgagcg
    12001 ttgccgctcc cggcataaca tgcgggtccg cgcagccgct gatttttatc ggattacccg
    12061 gcatcattat tcctccggct tcaatcaaca tatttctcgc ctgagtttgt ggcaaattaa
    12121 tggcctcagc cacacttaat aacggcgcga cgggcacacc aacttcatgt attttggcta
    12181 accaaacttc agctgcctgc gtttttaacg ttcgctcaat atattgttta agaatagcct
    12241 ggttttgtac gcgtaaaata ttgctgctaa atcggggatc attaaccagt tccgtaagct
    12301 ccagtgcctg gcataacgca gaaaaaagct tgtcattacc acaacaaatc gtaatcggct
    12361 tatcctgagt atcgaaaaca tcaaaaggtg ccatgtaggg atggcgattt cccaggcgtt
    12421 gtggtgactt ccctgtcgca atatatgcca tcagaccatg ctccagaaaa ctcagcgtgg
    12481 catcaaacat cgctatatcg acatgcgccc ctctctggct cttttcgcgg ccataaagtg
    12541 cactcactat tccgctgaat aaatagacac cgccgcatag atccgcaaga gaggtaccaa
    12601 cacgcactgg cggagcatca gggtaccctg tttccatcat tatcccactc attgcctgaa
    12661 tgatggtatc gtaggcagga gcatctttta gcggaccggt atgtccgaaa cctgacgatg
    12721 aagcatatat gaggcgcggg ttgatttctt gtaacctttc ccatgaaaac cccagttttt
    12781 ccattgtacc tgggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa
    12841 atatactttt atcgtgatca ttctttaaat caagaaccac actctctttg ccatgattaa
    12901 taaaactgta atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac
    12961 catgacctgg tggctcaact ttaattaccc ttgcgcccat attacaaaga agttgagttc
    13021 cgaaaggtcc attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt
    13081 tgctttcatt atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt
    13141 cggcatttat gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc
    13201 cacaagggag ccgtgacgac aaagcccagt acgctcacag ccaatgacgc tgtaccagta
    13261 cgggtgtaga cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg
    13321 cctgccagta ccatcatctg caaatgttcg ctgttcaaat gacatgccat accaacgaga
    13381 agcagcgcca gtggcatcag aatcaacttc aggaaggtgt tataagcaat ttcggcactg
    13441 aactcgaatt tatgtgcagc cagagtcaac cccgcagcga ataccgctac ccctgagtta
    13501 gccttcgcaa tcagattaaa ggtcgggtcc catgctgccg gaatttttac cccaaccagc
    13561 accaggatcg ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat
    13621 gcgctcagat tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga
    13681 ccaataggaa tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt
    13741 gatacggaat caccataaat aggatcgaga actgcaaacc ccaggaatcc aatggtaggt
    13801 gaacccgcaa ttaatgcaca tacagcggct tctgcatggg tacgtttaaa aaatttgtag
    13861 caaccaaacc aagagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga
    13921 cgagtgtccg caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca
    13981 ggaagagcgt agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt
    14041 tctcgtctgc cgctaaaata acccaacagc atgattacga taataggcaa taaatcgcca
    14101 ataaaaaatg ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag
    14161 ttggcaagag gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg
    14221 gtaatatggc cactttcagt tcctgcagca gggtcgatga ccacattaat aatggtcggt
    14281 ttgcgcgact gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta
    14341 tagccaacgc cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc
    14401 gttggtgatg gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca
    14461 ttattaaaaa taacgatcgt caccggcagg ttatatcgac aaatcgtttc aatttccatc
    14521 ccactgaaac caaaagcact atcaccttca atggcgacaa ccggagaacc agaagtcacg
    14581 ctagcaccga tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga
    14641 cgacgacgtg gtttatacat atcaataata tttcgtgcat tatccagggt atttgcacct
    14701 tcattaacta aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca
    14761 ttaaagtaat ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt
    14821 tgctgcttgt ggatatttaa aatatcgcgc cataccagtg gagtcgtaaa tgtgttttgt
    14881 ttcagttctg ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca
    14941 atggggcggt tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct
    15001 gccgcccatc cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg
    15061 acaacgtcag catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta
    15121 tcttcaagga tccctttcgc catagacatt ggcaggaatg gaatctgagc actttcaata
    15181 aattcacgaa gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat
    15241 ggccgttcag cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat
    15301 aatgctggcg acggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc
    15361 gtcgcggcca ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca
    15421 gagacccgga tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat
    15481 gctgctttgg catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc
    15541 tgtaggtcga cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt
    15601 accgttgcgt tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg
    15661 cagatccccg gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc
    15721 tcatgacgaa aaccaatata acgaatgcct tccgcctggg catggcgtgc catatccgtc
    15781 acaggaatac ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg
    15841 atgatatgca taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt
    15901 tttataaatt gggagccaga agcccccgtt aatatcagat ggcgtggttt tgcttcattg
    15961 cagcaatttc atcgtcgcta taacccagct cctgcaatac agcagcggta tgttcaccta
    16021 atagcggcgc agctttaata tccggcgtaa aggcagagaa tttcattgga caaccaacgg
    16081 tcagatattt tccacgcaac ggttgttcca cttcaacaac actgccactt tggcgcaaag
    16141 agggatcaag tgaaatttct ttcatactta aaaccggtgc acaaggaata tcaaactgag
    16201 tcaaataggc caccgcttca tgtttatcaa tagtgacagt gtatttttcg atttcagcaa
    16261 aaatatcgaa aatatgtggc tgtcgggcat gggctgtact gtatgccgaa tcggtaatcc
    16321 attctggttt gccgatggct ttacaggtgt tttcccagtt ttgctcctga atagtgaaat
    16381 aaatataggc gttaggatcg gtttcccagc ctttacattt caggatccag ccaggctgac
    16441 cgccaccacc tgcattacca ccgcggggaa ctgcatcacc aaatgtacca ttcggatact
    16501 gcgggtattc ttccagataa cccaatttat cgagacgctg ctggtcacgt aatttcacgc
    16561 ggcaaaggtt caatacggca tcctgcattg acatggtgac tcgttgccca cgccccgttt
    16621 tttcgcgatg cagcaaagca gcaagtaaac cgatcagcaa atgcattccg gtgttgctgt
    16681 cacccaacgc tgcagcgctt accagcggcg gaccatccca aaaacccgta gtggatgccg
    16741 cgccacccgc tgcctgagca acgttttcat aggcttttac attcacataa ggcgaacact
    16801 catcaaaccc tttgatcgaa ccaaaaatca gacgtggatt gatttcttga atatgctccc
    16861 aggtgaagcc catgtgatca atggcccctg gatgaaagtt ctcgactaag atatcagctt
    16921 cgcggatcag cttttccatt acctctttgc cttccgctgt tttggtattt aactcaatag
    16981 aacgtttgtt actgttaagc atggtgaagt aaagcgcatc gatatcagga atatctcgca
    17041 gttggtgacg cgttacgtca ccaacgccgg gacgttcaat tttaataacg tcagcgccaa
    17101 accaggccag catttgagta caagatgggc cagattgcac accggtgaaa tcgagaactt
    17161 taattccttg aagtggagtt gacatagcaa tacctattag ttaattaaat atgtgttaat
    17221 ttagcgttta tttaaggcat aacgccctct ataaataatc tgcaaaatat cttcgtagcg
    17281 gcactcgata tttttaaatt cctgtatcaa gtactgaaaa ttatttcatc gccacttcca
    17341 attcctttct gcatggtaag tagtgaggac gaaaagaaga atatgataaa
//
LOCUS       DAZPAC010000076        16879 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZPAC010000076 DAZPAC010000000
VERSION     DAZPAC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16879
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.076"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            450..602
                     /gene="mokC"
                     /locus_tag="ACHUPM_002118"
     CDS             450..602
                     /gene="mokC"
                     /locus_tag="ACHUPM_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR0869894.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(706..1836)
                     /gene="dnaJ"
                     /locus_tag="ACHUPM_002119"
     CDS             complement(706..1836)
                     /gene="dnaJ"
                     /locus_tag="ACHUPM_002119"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR0869895.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1925..3841)
                     /gene="dnaK"
                     /locus_tag="ACHUPM_002120"
     CDS             complement(1925..3841)
                     /gene="dnaK"
                     /locus_tag="ACHUPM_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR0869896.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4218..4622
                     /locus_tag="ACHUPM_002121"
     CDS             4218..4622
                     /locus_tag="ACHUPM_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR0869897.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4648..5361
                     /gene="msyB"
                     /locus_tag="ACHUPM_002122"
     CDS             4648..5361
                     /gene="msyB"
                     /locus_tag="ACHUPM_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR0869898.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5510..6076
                     /gene="satP"
                     /locus_tag="ACHUPM_002123"
     CDS             5510..6076
                     /gene="satP"
                     /locus_tag="ACHUPM_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR0869899.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6111..6698)
                     /gene="mog"
                     /locus_tag="ACHUPM_002124"
     CDS             complement(6111..6698)
                     /gene="mog"
                     /locus_tag="ACHUPM_002124"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR0869900.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6813..7766)
                     /gene="tal"
                     /locus_tag="ACHUPM_002125"
     CDS             complement(6813..7766)
                     /gene="tal"
                     /locus_tag="ACHUPM_002125"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0869901.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8045..9474
                     /locus_tag="ACHUPM_002126"
                     /pseudo
     CDS             8045..9474
                     /locus_tag="ACHUPM_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            9544..10320
                     /gene="yaaA"
                     /locus_tag="ACHUPM_002127"
     CDS             9544..10320
                     /gene="yaaA"
                     /locus_tag="ACHUPM_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR0869902.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10473..10769)
                     /locus_tag="ACHUPM_002128"
     CDS             complement(10473..10769)
                     /locus_tag="ACHUPM_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR0869903.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10983..12269)
                     /gene="thrC"
                     /locus_tag="ACHUPM_002129"
     CDS             complement(10983..12269)
                     /gene="thrC"
                     /locus_tag="ACHUPM_002129"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR0869904.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12270..13202)
                     /gene="thrB"
                     /locus_tag="ACHUPM_002130"
     CDS             complement(12270..13202)
                     /gene="thrB"
                     /locus_tag="ACHUPM_002130"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR0869905.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13204..15666)
                     /gene="thrA"
                     /locus_tag="ACHUPM_002131"
     CDS             complement(13204..15666)
                     /gene="thrA"
                     /locus_tag="ACHUPM_002131"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR0869906.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15693..15812)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15748..15813)
                     /gene="thrL"
                     /locus_tag="ACHUPM_002132"
     CDS             complement(15748..15813)
                     /gene="thrL"
                     /locus_tag="ACHUPM_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR0869907.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16027..16713)
                     /locus_tag="ACHUPM_002133"
     CDS             complement(16027..16713)
                     /locus_tag="ACHUPM_002133"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0869908.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3958 a         4447 c         4166 g         4308 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa taggcgaggc
      241 gtcttcagga ttagaatcga taaaacagaa tgggttaaag agagcgatat aatagcggcg
      301 ggtgcttgag gctatctgtc tcaggcatta gctgaacggc agatagagaa aagccccgag
      361 tgatatttta ctatcaaccc gaggccccct atatgctgaa cacatgtaga gtgcctctta
      421 ctgaccgtaa ggtcaaggag aagagagcaa tgaagcagca taaggcgatg attgtcgccc
      481 tgatcgtcat ctgtatcacc gccgtagtgg cggcgctggt aacgagaaaa gacctctgtg
      541 aggttcacat ccgaactggc cagacggagg ttgctgtttt cacggcttac gaatccgagt
      601 aagagcaacg gcggggagtg atccccgcca cttttcaggt gctcgcatat cttcaacgca
      661 ccctaatttt acccaagcct gcccacgggc aggcttttgg ggaggttagc gggtcaggtc
      721 gtcaaaaaac ttcttcacac catcaaagaa gctctttgag cgcgggctgt tgtgctcacc
      781 ggttgggcca ccgaagcttt cttgcagctc ttgcagcaac tgtttctgct tctcgttcag
      841 gcctaccggt gtttcgacga caacgcggca cagcaaatca ccctgtgcgc caccgcggac
      901 agacttgacg cctttaccgc gcatacggaa aagcttaccg gtctgggttt cgccaggcac
      961 tttcagtttg acgcgaccat caagggtcgg tacttcgatt tcgccaccca gcgccgccat
     1021 agcgaagttg atcgggactt cgcaatagag gttgttgcct tcacgctcga aaatcgggtg
     1081 ctgtttaacc tgaacctgaa cgtacagatc gcctgccggt gcgccatgct cgcccgcttc
     1141 accttcgccc gcaagacgga tgcggtctcc agtgtccacc cctgccggga ttttaacgga
     1201 cagcgttttg ctgcgctcaa cacgaccatg accatgacat ttgttgcacg gatctttgat
     1261 cagcgtaccg cggccctgac agtgtggaca ggtctgctgt acagcgaaaa atccctggcg
     1321 catctgcacc tgaccagaac catgacaggt cggacaggtc tgcggctgtg tacctggttt
     1381 tgcaccgcta ccgtggcaaa cgtcacactc ttccagagtc ggaatgcgga tctctttggt
     1441 cacgccacgt acagcttctt cgagggtgag ctccatgtta tagcgtaaat cagcaccgcg
     1501 cgccgcacgt tgacgaccac gtccgccgcc aaaaatatcg ccgaaaacgt caccaaaaat
     1561 atcgctgaag tctgcgccgc cgccaaaacc gccgccgccc atgccacctt gctcaaacgc
     1621 agcatgacca tactgatcgt atgccgcacg tttttgcgag tcggtcagaa cttcataagc
     1681 ttccttgatc tctttaaatt tcgcctcggc ctctttgtca ccctggttac ggtccgggtg
     1741 gtatttcatg gccaggcgtt tgtaggcctt tttgatttca cgctcttccg ctgttttgga
     1801 aacgcctaaa atctcgtaat aatcttgctt agccatcttt tttaaattgc ccctagatga
     1861 atgcacgggc ggagaggaaa ttccccttcg cccgtgtcag tataattacc cgtttatagg
     1921 gcgattattt tttgtctttg acttcttcaa attcagcgtc gacaacatcg tcatctttcg
     1981 cgttgtttgc agaagcatca gcaccggcag tctgctgctg ggcatgttgc tgctgggcga
     2041 tttccatcag tttctgggaa acctgtgcca gttcctgcat tttcgcttcg atagcggctt
     2101 tgtcttcacc tttcagagca gtttccagtg cagtcagcgc agactcgata gcagttttgt
     2161 cgtcagccgg cagtttgtcg cctgcttctt caacctgctt acgggtgctg tgcagcagat
     2221 ggtcgccctg gttgcgagtc tgtaccagct cttcaaactt acggtcagct tcggcgttag
     2281 cttctgcgtc gcgtaccatt ttctggattt catcttcgtt cagaccagaa gaagccttga
     2341 tggtgatctt ctgctcttta ccgctgtttt tatctttcgc ggaaacgtgc aagataccgt
     2401 cagcatcgat atcgaaggta acttcgatct gcggcatgcc gcgcggtgcc gggttgatac
     2461 catccaggtt gaactgaccc agagatttgt tatcagctgc acgtttacgt tcaccctgca
     2521 gcacatggat ggttaccgca gactggttgt cttcagcggt agagaacacc tggctgtgct
     2581 tggtcgggat agtggtattt ttcgcgatca gcgtcgtcat cacaccgccc atggtttcga
     2641 tacccagaga cagcggggta acgtccagca gcagtacgtc ttttacgtca ccagtcagaa
     2701 caccaccctg aacagcagca ccgattgcta cagcttcgtc cgggttaacg tctttacgcg
     2761 gctctttacc aaagaactca gcaactttct tctgaaccat tggcatacga gtctgaccac
     2821 ccacgaggat aacgtcgtcg atatcagata cggacaggcc agcgtcctgc agtgcaactt
     2881 tcagcggctc aatggaacgg tttaccagat cttcaaccag gctttccagt ttcgcacgag
     2941 tcactttgat gttcatgtgt ttcggaccgg tcgcatctgc agtgatgtac ggcaggttaa
     3001 cgtcggtctg ctgagcggaa gacagttcga ttttcgcttt ttctgccgct tctttcaggc
     3061 gctgcattgc cagcggatcg ttgcgcaggt caatgccctg atctttcttg aattcttcaa
     3121 ccaggtagtt gatcagacgg ctgtcgaagt cttcaccccc caggtgggta tcaccgttgg
     3181 tagccagaac ttcgaaggtt ttttcgccgt caacttcgtc gatttcgata atagaaatat
     3241 cgaaagtacc accacccagg tcataaaccg cgatagtacg gttgccagta cctttgtcca
     3301 gaccgtaagc cagcgcagct gcggtcggtt cgttgatgat acgttttact tccagaccag
     3361 cgatacggcc tgcgtctttg gttgcctgac gctgagcatc gttaaagtat gccggtacgg
     3421 tgataacagc ttcagttacc ggttcaccca agtaatcttc agcggttttc ttcatttttt
     3481 tcagcacttc agcagaaatc tgcggcggtg ccattttctg gcctttaact tcgacccatg
     3541 cgtcgccgtt atcagcagca ataattttga acggcatgat ggaaacatca cgctgtactt
     3601 cttcgtcctg gaagcggcgg ccaatcaggc gtttaatcgc aaacagggtg ttttgcgggt
     3661 tcgtcactgc ctgacgttta gccggctgac caaccagagt ttcaccatcc tgggtatagg
     3721 caataataga aggcgtggtg cgatcgcctt cggcgttctc cagcacgcga ggagtggtgc
     3781 catccataat cgctacacaa gagttggtag tacccaggtc gataccaatt attttaccca
     3841 tctaaacgtc tccactatat attcggtcat catgtggttg tgagtctgta ataggggcga
     3901 aacgtctggt ttcaactgcc caatttcatt tttttgcaga ctcactcact gcggttgact
     3961 actaaatggg gtcgtaaccc acgtcatcaa gggggagatg caaaaatttt ttgtgctttt
     4021 gcgcagaaat ttcgcgatat ggcaggacaa gacgtaaaat tgacagcaat tttattcata
     4081 taaagaatga atcgcagcca aggcattcat caaaaaattg taataaaaag aaaagattac
     4141 gtacctgaat cttctcttta tcagcagtaa actagtgggt attcatccct ctacctcttc
     4201 ccactaagag aatccttatg aaatccgttt ttacgatttc cgccagcctg gcgattagcc
     4261 tgatgctgtg ctgcacggcg caggcaaacg accataaact cctcggcgtc attgcaatgc
     4321 cgcgtaacga aaccaacgat ctggcgctga aacttcctgt ttgtcgcatt gtgaaacgca
     4381 tacaactctc tgccgaccat ggcgatttac agttaagcgg tgcatcggtt tatttcaaag
     4441 ccgcccgtag cgccagtcag agtctgaata ttccttcaga aataaaagaa gggcaaacca
     4501 ctgactggat caacattaac agtgataacg acaataaacg ctgcgtctca aaaatcacct
     4561 tttccggtca tacggtgaac tcatcggata tggccacgct gaaaattatc ggcgacgact
     4621 aacgccagaa tatagaaacc acaaaaaatg aatgttaatt acctgaatga ttcagatctg
     4681 gattttcttc agcattgtag tgaggaacag ttggcaaatt tcgcccgatt gctcacccat
     4741 aatgaaaaag gcaaaactcg cctctccagc gtactgatgc gcaacgaact gtttaaatcg
     4801 atggaagggc atcccgagca acatcgccgc aactggcagc tgattgctgg agaattacag
     4861 cattttggtg gcgatagtat cgccaacaaa ctgcgcggac acggtaaatt gtatcgggcc
     4921 attttgctcg atgtttcaaa acgattgaag ctgaaagccg acaaagagat gtctacgttt
     4981 gagattgagc aacagttact ggaacaattt ctgcgtaaca cctggaagaa tatggacgag
     5041 gaacataagc aggagtttct gcacgcggtc gatgccaggg tgaatgagct ggaagagctg
     5101 ctgccgctgc tgatgaaaga caaattattg gcaaaaggcg tgtcgcattt gctttccagc
     5161 caattgaccc gcattttacg cacccacgca gcaatgagcg tgcttgggca tggtttgctg
     5221 cgcggcgcgg gtctgggagg ccctgtgggc gcagcattaa atggggttaa agcggtcagc
     5281 ggcagtgcct atcgcgtgac aattccagcc gtaatacaaa tcgcctgcct gcgccggatg
     5341 gttagcgcca ctcaggtctg acaccgtaaa ccggggaaaa ttttcatttt cccctcaaaa
     5401 gatcgtagac actgccccac tggctgatta ttatgccgcg ccctgaaaac actactgtta
     5461 ttcagggaaa ttatttcacc attcattcga tgatgatttt tgaggaatta tgggcaacac
     5521 taagttggct aatccggcac cgctgggcct gatgggcttc ggcatgacca ccattctgct
     5581 taacctgcac aacgtgggtt atttcgctct ggacggtatt attcttgcca tgggcatttt
     5641 ctacggcggc atcgcgcaaa tttttgccgg tctgctggag tacaaaaaag gcaacacttt
     5701 cggtttaacc gcattcacct cttacggttc tttctggttg acgctggttg cgattctgct
     5761 gatgccgaaa ctgggtctga ccgatgcgcc aaatgcacag ttccttggtg tctacctggg
     5821 tctgtggggc gtatttacgc tgtttatgtt cttcggcacg ctgaaaggcg cacgcgttct
     5881 gcaattcgtt ttctttagcc tgactgtgct gtttgccctg ctggcgatcg gtaacattgc
     5941 cggtaacgcc gcaatcatcc actttgccgg ctggattggt ctgatctgtg gtgccagtgc
     6001 aatctatctg gcgatgggtg aagtactgaa cgagcagttt ggtcgcaccg ttctgccgat
     6061 tggtgaatcc cactaattgt tttgagatcc ccccgctcgg ggggattttt ttattcgcta
     6121 acgtcgcgtc ttgcactctt cggtctgaat gcagcaacca cttctggtgc cgtttcaacg
     6181 tatggccctt ccagcaactg aatgcagtac ggtacgctgg caaaaatacc gtgtaccaca
     6241 acgttacccg cagcgtcctt cacaccttcc agcgtctctt taatagactt cggctgaccg
     6301 ggtaagttaa ggatcagcgc ctgtttgcga atcacaccaa cctgacgcga aaggatcgca
     6361 gttggtacaa aatgcaggct tatctggcgc atctgttcac caaagccagg catctcgcgg
     6421 tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac gcgccgggcc agttccgccc
     6481 gtggtgagca ccagatggca actcatttca tccactagct cacacaacgt ttgctcgatg
     6541 atcgcctgct catcggggat taagcgggtt tccagttcaa acggcgtggt tagcgccgat
     6601 gtcagccatt cttccagcgc agggatgcct ttatcctgat aaacgccgct ggatgcgcga
     6661 tcagagatgg aaactaagcc aatacgtaaa gtattcatca agatttttcc gtttaaacag
     6721 tctcgttaaa cagaatgata cactgcgaag ggagtgacag acaggcttca gaagaggtag
     6781 cgtgaccgac ttcccggtca cgctaagaat gattacagca gatcgccgat cattttttcc
     6841 agtttttcct ggtcaatagc aaacttacgg ataccttccg ccagtttatc tactgccatt
     6901 ggatcctggt tgtgctgcca caggaactcg gactcagtga tacgcgccgg acgcgctttc
     6961 acttcgccgg tgtaagacag tttacgttcg atagcccctt cgctctcagc cagctctttc
     7021 agcagtgccg gtgcgatggt aagacggtcg cagcctgcca gttccagaat ttcgccgatg
     7081 ttacggaagc ttgcgcccat aaccacggtt tcataaccgt gctctttgta gtactggtag
     7141 atttcagata cagaaaccac gcccggatct tctgccggag cgtactcttt cttatcagta
     7201 ttcgctttgt accagtcaag aatacggcca acaaatggcg agatcaggaa cacgcctgct
     7261 tccgcacaag cacgagcctg agcgaaggag aacagcaggg tcaggttaca gttgatgcct
     7321 tctttttcca gctgttctgc agcacggata ccctgccaag tagaagccag tttgatcaga
     7381 atacgatcgt tgctaatacc agcatcgttg tagagtttga tcaggcgttt tgctttcgca
     7441 attgacgctt cggtgtcata ggaaagacgc gcatcaactt cagttgagat acggcccgga
     7501 accagtttca ggatttccag accaatattt actgccagtt tgtcggtcgc gtccacgatc
     7561 tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga cagcatcatc aatcaactta
     7621 cggtattccg gaatctgcgc tgcgttaaga atgagagaag ggttggttgt ggcatcctgc
     7681 ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg ccactacggt ggtgtactga
     7741 cgaagggagg tcaatttgtc cgtcatgata gtatttctct ttaaacagct tgttaggggg
     7801 atgtaaccgg tctgccctga tgatatcacg acgcaacgtc agcgcaaccg ccgacaagac
     7861 ggttatcgcc aggcgagact gtttcggatt tctgacactg cgttgatatc gcccgccatt
     7921 tttatacaaa acctcatgta tgctacgcag aagttatcaa gtacctcgta gcgtatatac
     7981 ttcttaaaca attggtaacg tttacacagg aaagtcatcg cgaccggcaa taagagggat
     8041 atgcatgcca gattttttct cctttattaa cagcgtcctt tggggatcgg taatgattta
     8101 cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc ggatttgtgc agtttcgcta
     8161 catccgccag tttggcaaaa gtcttaaaaa tagcattcat ccacagccag gcggtttaac
     8221 ctcatttcag tcattgtgta ccagtcttgc ggcgcgcgtg ggtagcggca acctggccgg
     8281 cgttgcgctg gctattaccg ccggtggacc tggagccgtc ttttggatgt gggttgccgc
     8341 gtttatcggc atggcgacct cgtttgccga atgttccctt gcacaacttt ataaagaacg
     8401 tgacgtcaat ggacagtttc gtggaggacc ggcatggtat atggcgcgcg ggctggggat
     8461 gcgttggatg ggcgttctgt tcgccgtctt tttgctcatc gcctacggca taattttcag
     8521 cggagttcag gcgaacgccg ttgcccgagc cctgagtttt cttttgattt tcccccgctg
     8581 gtgacaggca ttattctcgc tgtctttgct ctgctggcaa tcactcgcgg tcttcatggc
     8641 gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga taatctgggt gctgaccagc
     8701 atggtgattt gcgtaataaa tatcgggcaa cttccccacg tcatttggtc tatttttgag
     8761 agtgcttttg gctggcagga agcggcaggc ggcgcggcgg gatatacctt aagccaggcg
     8821 attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg ttcgacgcca
     8881 aacgcggcag cggcagcggc gtcctggcct ccgcatccgg cagcgcaagg gattgtccag
     8941 atgattggca tttttatcga caccctggtc atctgtacgg caagcgccat gctgatatta
     9001 ctggcgggta acgacacaac ctacatgccg ctggaaggta ttcagcttat ccagaaggcg
     9061 atgcgggtgc taatgggttc ctggggtgct gagtttgtta ccctcgtggt tattctgttt
     9121 gccttcagct ccatcgttgc caactacatt tatgccgaaa acaatctctt ctttttacgc
     9181 ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac ggtcatcggc
     9241 ggcaccttgc taagtcttcc gctgatgtgg caactggcag atatcataat ggcctgcatg
     9301 gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac cattgccagt
     9361 gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt gcgttatccg
     9421 gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga gtaatcacaa
     9481 ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct gcaaggactg
     9541 gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc gttgaccacc
     9601 acgcgctata cgctgccgga gctgttagac aattcccagc acttgatcca tgaagcgcgg
     9661 aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact ggcgggtatc
     9721 aacgccgctc gctttcatga ctggcagcca gatttcacgc cggagaatgc ccgccaggct
     9781 attttggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacttt cagcgaagac
     9841 gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg cgtactgcgc
     9901 ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga gaatgcccga
     9961 gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa cgaggcgctc
    10021 gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt taaatcggtg
    10081 aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga gaagaacggc
    10141 aaatttaaga taatcagctt ctacgctaag aaagcacgcg gtctgatgag tcgtttcatt
    10201 attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga aggttacttc
    10261 tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga gcagcgttaa
    10321 gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcatgcaga
    10381 ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg catcaggcgc
    10441 tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc tggaccatga
    10501 ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg cggtccgtgt
    10561 gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg gtcgcgccag
    10621 tgacctccat cccagtaata gccacgatta tcacgatcgc ctatctgtaa ttttactgac
    10681 gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac cagggaaagt
    10741 gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg cccgttatgg
    10801 gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca atcctaatct
    10861 ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttttc tccataattt
    10921 cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat aatgaataga
    10981 ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg cagattatgt
    11041 gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa cgtttcaccg
    11101 agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag gaacaagcca
    11161 tattcgcctg gattcaactg gtcacacagc gcacgatacg ctacggcagc gtgcggctcc
    11221 gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc atcatccacg
    11281 gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc ttccacacgc
    11341 ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg agtcgctttg
    11401 ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt ggtcgcagca
    11461 ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt caaatcgccg
    11521 aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg cagctgcgca
    11581 actgcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat cgagttagcc
    11641 gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt caccagcgcc
    11701 tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc caacgtacag
    11761 aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac cactttcaca
    11821 ttcggcaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga ggtcgcggtc
    11881 agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat aaagcgaccg
    11941 ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc gacatcgctt
    12001 tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc ttccaggatt
    12061 tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg ggtgacaaaa
    12121 tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc atgcggaaaa
    12181 aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct gacctgctcg
    12241 ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc gcccgccgta
    12301 tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt acccaaccag
    12361 tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt cgggccggag
    12421 ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc ctgccggaag
    12481 ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag cttcgcggca
    12541 agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg cccgtgcgca
    12601 atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc cgtcgagact
    12661 ttaattcccg gatacgccag cacccacagc cactcatcaa accctggcac ttgctggctg
    12721 atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg tgccacgttg
    12781 tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc ccatcaaagc cagcaaacgg
    12841 gtgtcattaa gtggcttgcc gcagtgttca ttcatcgcca tcagcgccgc gaccaccgaa
    12901 caggcgctgg agcctaagcc cgagccgatc ggcatattct tttccagagt catcgccact
    12961 ggaatttgct tgcccagctc caggcaaaaa cgctcccagc actgataaac gatattttcc
    13021 cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact gaatgtctct
    13081 gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacgggtgt caccgccgcc
    13141 ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata aactttaacc
    13201 atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga caccagcagc
    13261 ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct gataatagtg
    13321 gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat cattcccatc
    13381 cacttcggca atcttcacgc ggcagacacc atcttcatca atattgccaa catagcgcaa
    13441 aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt cgagctgtga
    13501 cagattcgcc ataaaagcgg caacatcacc ctcggcgtta aactctgcgg gcagcacagg
    13561 ttcaatttca atatccgcca gttccagttc acgtcccgtt tcacgagcga gaatcaatag
    13621 tttacgcgcc acatccatac cagaaagatc atctcgcggg tccggttcgg tataacccat
    13681 ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct tcgtctaact tgccgaagat
    13741 ataagaaagc gaacctgaaa gaatgccgga gaacttcacc aattcatcac ctgcattgag
    13801 cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt catagaggaa
    13861 tttacgccgc gatttttccg ccgcataacg caactgatga tagtaatcca tcgacgaggt
    13921 gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt cggcatattg
    13981 atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca gatgatattc
    14041 tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgtg caagttcttc
    14101 ctgccagttt tccagattaa ggccatgcac actagtgagc agagccttcg agttggcaac
    14161 gccgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgctgac gcttcagttg
    14221 ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga taacctgatc
    14281 ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat cgttatttac
    14341 cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt tgatattggc
    14401 gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca taccatcacc
    14461 taccaccgag ataatggcca gccgttccat caccgccagc ggctccagta agccttcttt
    14521 cagttccagg tagaactctt cctgcattgc ccgttcagcc cgcacacagt cgctttgcgg
    14581 aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca cggaaatacg
    14641 ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt tcatccccgg
    14701 gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga ccggtaattc
    14761 gccttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc cggtattttt
    14821 aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt gaagaacttt
    14881 agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca acaacctcgc
    14941 atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc aaatctcgca
    15001 acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac cgttgcgtcc
    15061 aagtaccacc agttcgcctt tttcattacc ggcggtgaag cctgccatca gcaccatgtg
    15121 atcagccgga atacgacttg ccgcaatacg gcgggtggac tcagcaatat cgacggtaga
    15181 ttcgaggtaa tgccccactg ccagtagttt ttcgaccgga tcgataacgg taacgttgtg
    15241 accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc cacggcaaat
    15301 cagcgcagcg ttaatgctat ccgggcactg ccctaacaaa ctaatgccat gcaggacatg
    15361 ttttatttgg gcaaattcct ggtcgacgaa agttttcaat tgcgccagcg ggaatcccgg
    15421 ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat cgctgatatt
    15481 gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt ggttggtgat
    15541 tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc tttccagaat
    15601 atcggcaacc cgcagaaaac gttctgcatt tgccactgat gtaccgccga acttcaacac
    15661 tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa agcccgcact gtcaggtgcg
    15721 ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg taatggtgat
    15781 ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg taatttttat
    15841 ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc aataaaattt
    15901 taatttactc atggcaggta accagttcag aagctgctat cagacactct ttttttaatc
    15961 cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata cttactaagg
    16021 cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca acattgccgt
    16081 gtctcgttgc tctaaaagcc ccaggcgttg ctgtaaccag tcgaccagtt ttatgtcatc
    16141 tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt gcagttgatg
    16201 ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta attgatagca
    16261 atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca tcggcacacc
    16321 agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt cgcgaccaaa
    16381 caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca ccagttctac
    16441 tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga cagtgaaatc
    16501 gacatcgtgt aacgattcag ccagtgttgg gaaaacttta atattatcaa taacatcacc
    16561 agatccatgt gcgacccagc gggtggctgg ctccaggtgt gcctgactat cgacaatccg
    16621 cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat tttctgctct
    16681 ggcgggtgcg accaggataa tcgttatacg catattgcca ctcttcttga ccaaataacc
    16741 gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat tcttaacgta
    16801 agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc tgactgtact
    16861 aacggtttaa ttgttaaaa
//
LOCUS       DAZPAC010000077        16864 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZPAC010000077 DAZPAC010000000
VERSION     DAZPAC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.077"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            92..1132
                     /locus_tag="ACHUPM_002134"
     CDS             92..1132
                     /locus_tag="ACHUPM_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR0869909.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHUPM_002135"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHUPM_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR0869910.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHUPM_002136"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHUPM_002136"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR0869911.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="ACHUPM_002137"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="ACHUPM_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR0869912.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHUPM_002138"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHUPM_002138"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR0869913.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="ACHUPM_002139"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="ACHUPM_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR0869914.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9086)
                     /locus_tag="ACHUPM_002140"
                     /pseudo
     CDS             complement(8439..9086)
                     /locus_tag="ACHUPM_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9217..10635)
                     /gene="araE"
                     /locus_tag="ACHUPM_002141"
     CDS             complement(9217..10635)
                     /gene="araE"
                     /locus_tag="ACHUPM_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR0869915.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="ACHUPM_002142"
     CDS             complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="ACHUPM_002142"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR0869916.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11776..12612)
                     /gene="kduI"
                     /locus_tag="ACHUPM_002143"
     CDS             complement(11776..12612)
                     /gene="kduI"
                     /locus_tag="ACHUPM_002143"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR0869917.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12899..14080)
                     /locus_tag="ACHUPM_002144"
     CDS             complement(12899..14080)
                     /locus_tag="ACHUPM_002144"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR0869918.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14335..15564
                     /locus_tag="ACHUPM_002145"
     CDS             14335..15564
                     /locus_tag="ACHUPM_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0869919.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16024..16656
                     /locus_tag="ACHUPM_002146"
     CDS             16024..16656
                     /locus_tag="ACHUPM_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0869920.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT         4450 a         4424 c         3897 g         4093 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctag gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acatggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggtacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt attccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgcc
     8821 actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg ctgtaagcca
     8881 agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg ctggcactct
     8941 tctatttcat gaaaatcgac gctatgtagc agcacttgcg cagagtgaag cccaccaagc
     9001 cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc ccagctcatt
     9061 cctcccagca aaccaattgt tttcattcct ctctctcctg atgtgtgtta ccgcaacaag
     9121 gctgaataag gaacgagaca aacgcctcaa cggccaggtg cccaatctct attaacgaaa
     9181 aaagggccgg atgtacagca catccggccc gtgaaatcag acgccgatat ttctcaactt
     9241 ctcgcctgcc atcagtttgc gttcgatatg ttccagcgtg acatttttgg tttccggaat
     9301 gagccagaaa gtgatgccca caaacgcaat gttcagcgca gtgtagagcc agaacgtacc
     9361 ggcagcacca atgctgtcga gcagcgtcag gaaggtcgcg ccgataatca tattcgacac
     9421 ccagttcgtg gtggtcgaac aggtaatacc gaaatcgcgg catttcagcg gctgaatttc
     9481 agagcacagg atccacacca ctggcgcggc gctcatcgca taaccggcaa tacacatcat
     9541 cgtcatgcca acagagagcc aggacaagcc actggaagcc gtaccgttat caaactgcat
     9601 caggcaatag cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc
     9661 cggtttacgc cctgctttat ctaccgtaaa caccgcaata aaggtggcga acataaaggt
     9721 cagccctacg accagagtcg caatcatctg ttgttctgtg gtcgtaaagc ccgccatttt
     9781 gaagatacgc ggcgcgtagt acatgatgat gttcataccg gtaaactgct gcatcgcctg
     9841 caacaacata ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa acagtgccca
     9901 accgccctgt tttaacttca ggctttcacg aatttcgttg agttcttctc gcgctttttc
     9961 cgacgtatcg cgcagcatac gcaacacttc ttccgcctca atatgacgcc ccttttccgc
    10021 cagccagcgc gggctatttg gcaggaagac caccagaata atcagcagga ctgctggtaa
    10081 agcaagaacc cccaacattg cgcgccagtt accgctataa ctgaacgctg tatcggataa
    10141 aaacgccagc acgatgccga gtgtgaccat caactggtac atactgatca tcttaccgcg
    10201 aacgttttca cttgccattt cagaaagata cagaggagcg gtgtaagacg cgatcccgac
    10261 agcaatgccc agcaccaccc gagcggcgat taacatctct acgctggtcg caaaagcgga
    10321 ccctatagaa ccgagtacaa acaggatggc ccccgccatc aggctgtatt tacgccccag
    10381 gcggaacgac agccaaccat taaacagcgc accaattgct gcgccgagca tcatgctact
    10441 aaccacccat tcctgcaaac gactggtcag cacaaagtga tcggtaatga acggcaacgc
    10501 tccggcgatt acgccgatat caagaccaaa taacaatcct gcgaccgcag cagctaccga
    10561 aacaaacata ttcatacgcc gcgtatcccg caaagaacgt ggcgttaaag cagattccgt
    10621 attgatagta accatttttt cctgccagca gagagtaaga catagtgaaa aaatacgtga
    10681 acaactcaca caggtgtcag gtcggaaaca gcataaatat ggattaaatt gctgcgacat
    10741 gtcgttatgt gatggatatt ccaattttca aattaagttg aattattgag attattatta
    10801 accacctaat tttacagcag ataaaattca taaagttcat taattgataa ttgatatgga
    10861 ttatttcata accatgatat gaattatgat gacctacagg tataaaaaac cctgccatga
    10921 ggcagggtca taaaagtaag aggaatgaat taacgagcca gccaaccgcc atctacggca
    10981 atggtataac cattcacata atctgaagcg ctggaggcaa ggaacactat cggccccatc
    11041 aggtcactcg gcagtcccca acgaccagct ggaatgcggt cgagaatttc cgcgctacgt
    11101 tgttcatctg cccgtagttg ttgagtattg ttggtcgcca tgtaacccgg agctatcgca
    11161 ttaacattaa tgttgtgttt agcccattcg ttcgccatca atcgcgtcac gcccatcacg
    11221 ccgctttttg atgcggtata agaaggcaca cggatcccgc cctggaagga gagcattgac
    11281 gtgatattga taatcttgcc gccattgcgt tgcgcgataa agtgtttcgc cgctgcctga
    11341 gacatgaaga atacgctctt gatattcagg ttcatgacat cgtcccagtc tttttcgctg
    11401 aactcgagag catcttcgcg gcgaatcaat ccggcgttat tcaccaggat atcaatatga
    11461 ccaaactcag ctaccgcgcg atccagcagt gctggaatgc catcaatctt tcgcagatcg
    11521 gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct gcttgatggt ttcagtcggt
    11581 tcaacgatgt taatgccaac aatgtcacag cccgcttgcg ccagccccaa cgccatcccc
    11641 tggcccagtc cagtatcaca accagtgacg accgcaactt taccttcgag agaaaatgca
    11701 cttaaaatca taacaatacc ttattcgttt atgccgacaa ctagcgcaaa tctttaacgg
    11761 ccactatgcc gacaactagc gcaaatcttt aacggccaca tggtccatat catcaaagac
    11821 ctggttttca ccaaccatgc cccagataaa ggtataagct ttggttccga cgccggaatg
    11881 gatcgaccag ctgggggaga tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc
    11941 ttgcggctgc cccatcatgt ggaaaacgca ggcgtcatca tccatattga aatagaaata
    12001 aacttccatc cggcgctcgt gagtgtgaca cggcatggtg ttccacaagt tacctggagc
    12061 cagctccgtc agccccatac tcaattggca ggtttccagc acatccggga caaaatattt
    12121 gttaattgtg cgacggttac tggtgaggtt atcgcctaac gtgactggag atacttcgtc
    12181 cggtgtgact tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt
    12241 tgccggagtg gcggtatcaa tactggcaaa gacaacttct tttgcacctt taccaacata
    12301 cagggcgtcg cggtgaccga tttcatagca ttggccatcg acagtaatcg taccggcacc
    12361 gccaatattg ataacaccta actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt
    12421 accaacttcc ccgccaacgg aaaccgtttt agttatcggc ataatgccgc caacaataat
    12481 tcggtcaatg tggctgtaaa ccatggtgta ctcatcggcg acaaatacct tttcaaccaa
    12541 aaattcattg cgcagccctt gggtatccag cgtttttgcg tgcgcactgt ggatgctctg
    12601 tcttacgtcc acatcaacct ccgataatca gtgaagcgtg aaaatagaaa acgatagtga
    12661 aacactgttt cgttttctct ataaagggat gatatgccca tgggctaggg ttttcaatta
    12721 aaaataaaac aatgtttcat ttttaagtta aggattaaaa aaagtgcagt gtcgatcacg
    12781 aatatgtcat gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc
    12841 atcattattc aacagattaa tgacggcaga tatttatcgg taaggaggaa atgcagagct
    12901 actcgtcacg ttcaatggtc aatgccacgc cctggccccc gccgatacaa agcgttgcca
    12961 gccctttgcg ggcattacgt ttcaccattt catgaaccag agaaaccagg attcggcaac
    13021 cggaagcgcc tatcgggtga ccgagtgcga tcgcgccacc attgacattg acccgacgct
    13081 catcccactc aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct
    13141 cgataagatc gacatcagcc aactgccagc ctacacgctc caggcaacgg cgggtcgcat
    13201 acaccggcgc aattcccatc aatgccggat ctacaccaac gctggcaaat gcgcggatgc
    13261 gggccagcac gggtaaattc aacgctcgtg ctttggcttc gctcatcatc attaccgcag
    13321 ctgcgccatc gtttatggat gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg
    13381 aaggatttaa acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat
    13441 caacaaccaa cgtctgaccg ttactttgga tcattaccgg gacgatctca tctttaaatc
    13501 gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca
    13561 actgacggct gatgccatat tcgcgagcca gattttcggc ggtgacacca atatgataat
    13621 cattgaaggc atcccacaac ccatcatgca ccagactgtc aaccaactgg ctattgccaa
    13681 gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg ttttcctggc
    13741 caccggcgat gacaatatca gcctcgccac actgtatcgc ctgagtagcc agatgcagtg
    13801 ctttaagtcc ggaaccgcaa acgtcattaa tcgtgattgc agaaacgcta ttaggaagac
    13861 cacctttaat agccgattgc cttgccggat tctgccctgc ccctgcagtc aacacctgac
    13921 caagaattac ttcatccacc gcatatgcag gaacgccggt acgttctatt aacgctttca
    13981 cgaccagact accaagttcc acggcggaat gacccgctaa cgcaccacga aagcagccga
    14041 taggtgtccg taacgccccg acaatcacaa cgtctttcat cacaacctcg caccaattaa
    14101 cagtgcaata gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa aaagtgattt
    14161 ttatcacaaa ggaaatatgc ctgagcagta gtcagagtca taactggcac gtaaggtttg
    14221 caaccactaa cccaccaata gatgggtaga taaggcgtta atctcccata cttaacctgg
    14281 tttatggtaa attgccctcc attttgttta atttgtagat gatacgttca gataatgtct
    14341 aatatttggt ctaaagaaga aactctgtgg agtttcgcgc tctacggcac agccgttggt
    14401 gcaggcacgc tcttccttcc tattcagtta ggttcggcag gggctgtggt cctgtttatt
    14461 actgctctgg tcgcctggcc tttaacatat tggccacata aagccttatg ccagttcatc
    14521 ctctcatcaa aaacatcagc atgtgaaggg ataacgggcg cggtaacaca ctactatggc
    14581 aagaagattg gtaatctgat taccacgctg tacttcatcg ccttttttgt cgtcgtgttg
    14641 atatatgcag tggcaattac caactcactt acggaacagc tggcaaagca tatggttatt
    14701 gatcttcgca tccgtatgtt ggtgagtctg ggtgttgtat taattctgaa tctcattttt
    14761 ctgatgggac gtcatgccac tattcgggta atgggatttt tggtattccc attgattgcc
    14821 tatttcttat ttctttccat ttaccttgtc ggtagttggc aacctgatct attaacgacc
    14881 caggtagagt tcaatcagaa tacccttcac cagatatgga tatcgattcc cgtgatggtt
    14941 ttcgccttta gccatacgcc cattatttct acgtttgcca tagacagacg tgaaaaatat
    15001 ggcgaacacg ctatggataa atgcaaaaaa attatgaaag tcgcttatct catcatctgc
    15061 ataagtgtac tgttctttgt ctttagctgc ctgctttcta ttccaccttc gtatattgaa
    15121 gcggctaaag aagaaggggt caccatttta tcggcgcttt ctatgctgcc gaacgcccca
    15181 gcatggttgt caatttccgg gattattgtc gcagtagttg cgatgtcgaa atcattcctg
    15241 ggtacgtact ttggcgttat tgaaggtgcc acggaggtcg tcaaaacaac actacagcag
    15301 gttggtgtaa agaaaagtcg tgcatttaac cgcgcactat caattatgtt ggtatcgctg
    15361 attaccttca ttgtttgttg cattaacccg aacgcgattt cgatgattta tgcgatcagc
    15421 ggcccgctca ttgccatgat acttttcatc atgcctacgt tgtcaacgta tctgatcccg
    15481 gcgcttaaac cctggcgctc catcggaaat ctgattacgc tgatcgtggg tatcctgtgc
    15541 gtatcggtaa tgttctttag ctaatatccg ttgtggatgg ttcaaaattg ctacaatttt
    15601 gaaccattcc atttttcata atattcattt gttattattc cgccctatca cttctctcgt
    15661 catatcctag aattatcagt tagttaaaca cacaaagaga tctgaacatc ttaaataaga
    15721 ctatttaaga tgcataactt agattcgcaa gatatcttct ggtcattcaa aaacaatttg
    15781 cgatatagat attctcatac catgagatag ttatctggac cacgttcgac catgcaagat
    15841 taaatcagtg aaacataatc atatttaata acgagaaatg catttttaaa tgtcttaatc
    15901 agaattatag caaatacaga ttaatcgcaa tttttcccag caaaattaca aaatcaagat
    15961 gtattgacat catcaatcac caatgaaatt atgcaattat atacggatag ggaggttctt
    16021 aacatggggc tttgtagtcg ttataaaagt cttacatgca atagttgctc aatgcattgc
    16081 caaataatgc cagaagagtc accgcgtttg cagtattgcg caaactcgtg tttttgtatg
    16141 tggcccgaag aaagttcata ttttaatcgt ggtgtggtgg aaggtatctt aacaaaaaac
    16201 cacaatgcga gattaagcgg atatattttt gtggattttt cagtaagttt tttacgtcta
    16261 ttccttgaaa aagactggat tgactatctt gcaagtactg acatggggat agttttagtc
    16321 agcgaccgca atatgcaatc gttagctaac tactggcgaa aacacaacac cgcaatatct
    16381 gctgtcatat ataatgatga tggtcttgat gttgccaatg aaaagatcag acaactattt
    16441 attggtcgtt atttatcatt taccagaggt aacacattaa ctcagatgga attcaccata
    16501 atggggtata tggtttctgg ttataatcca tatcagattg ctgaagtgct ggatatggat
    16561 atccgtagca tctacgcgta caagcaacga atcgaaaaga gaatgggtgg taaaataaac
    16621 gaattattta ttcgttcaca ttcggtccaa cattgatact caacgaccag ccagaatcat
    16681 actctgttca tacgggcatc tattgaaatg cccgtatttt ataaataagc agtaacccca
    16741 agagtaaaca acatagtatt taaaaccatc acacgaaaaa ttcagaagca ttacgaaata
    16801 tgaatttaca aacaatagtg gcataaatgt taaccatgtt aatttacgta aagttttacg
    16861 ttgc
//
LOCUS       DAZPAC010000078        16849 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZPAC010000078 DAZPAC010000000
VERSION     DAZPAC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16849
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.078"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            297..1280
                     /locus_tag="ACHUPM_002147"
                     /pseudo
     CDS             297..1280
                     /locus_tag="ACHUPM_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(1596..2798)
                     /locus_tag="ACHUPM_002148"
     CDS             complement(1596..2798)
                     /locus_tag="ACHUPM_002148"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR0869921.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3030..4529
                     /gene="pitA"
                     /locus_tag="ACHUPM_002149"
     CDS             3030..4529
                     /gene="pitA"
                     /locus_tag="ACHUPM_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR0869922.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4773..5108)
                     /gene="uspB"
                     /locus_tag="ACHUPM_002150"
     CDS             complement(4773..5108)
                     /gene="uspB"
                     /locus_tag="ACHUPM_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR0869923.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5499..5933
                     /gene="uspA"
                     /locus_tag="ACHUPM_002151"
     CDS             5499..5933
                     /gene="uspA"
                     /locus_tag="ACHUPM_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR0869924.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(6032..6085)
                     /locus_tag="ACHUPM_002152"
     CDS             complement(6032..6085)
                     /locus_tag="ACHUPM_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869925.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            6251..7720
                     /gene="dtpB"
                     /locus_tag="ACHUPM_002153"
     CDS             6251..7720
                     /gene="dtpB"
                     /locus_tag="ACHUPM_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFR0869926.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7769..8521)
                     /gene="rsmJ"
                     /locus_tag="ACHUPM_002154"
     CDS             complement(7769..8521)
                     /gene="rsmJ"
                     /locus_tag="ACHUPM_002154"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR0869927.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8529..10571)
                     /gene="prlC"
                     /locus_tag="ACHUPM_002155"
     CDS             complement(8529..10571)
                     /gene="prlC"
                     /locus_tag="ACHUPM_002155"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR0869928.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10774..11616
                     /gene="rlmJ"
                     /locus_tag="ACHUPM_002156"
     CDS             10774..11616
                     /gene="rlmJ"
                     /locus_tag="ACHUPM_002156"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR0869929.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11688..13040
                     /gene="gorA"
                     /locus_tag="ACHUPM_002157"
     CDS             11688..13040
                     /gene="gorA"
                     /locus_tag="ACHUPM_002157"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR0869930.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(13094..13177)
                     /gene="dinQ"
                     /locus_tag="ACHUPM_002158"
     CDS             complement(13094..13177)
                     /gene="dinQ"
                     /locus_tag="ACHUPM_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR0869931.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13205..13378
                     /locus_tag="ACHUPM_002159"
     CDS             13205..13378
                     /locus_tag="ACHUPM_002159"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0869932.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            13917..14270
                     /gene="arsR"
                     /locus_tag="ACHUPM_002160"
     CDS             13917..14270
                     /gene="arsR"
                     /locus_tag="ACHUPM_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR0869933.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            14324..15613
                     /gene="arsB"
                     /locus_tag="ACHUPM_002161"
     CDS             14324..15613
                     /gene="arsB"
                     /locus_tag="ACHUPM_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR0869934.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            15626..16051
                     /gene="arsC"
                     /locus_tag="ACHUPM_002162"
     CDS             15626..16051
                     /gene="arsC"
                     /locus_tag="ACHUPM_002162"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR0869935.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(16185..16481)
                     /locus_tag="ACHUPM_002163"
     CDS             complement(16185..16481)
                     /locus_tag="ACHUPM_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR0869936.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            16588..>16849
                     /locus_tag="ACHUPM_002164"
     CDS             16588..>16849
                     /locus_tag="ACHUPM_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0869937.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
BASE COUNT         3888 a         4377 c         4272 g         4312 t
ORIGIN      
        1 attttaagca ccgagactat ccggtctttt actaaataat ggaaaacaat ccatccctgg
       61 aagataattg taaaaaaaac aacagaataa gaattaatct taggataaat atttatttat
      121 catggccttt taaccggtaa aaaatggcac tcggcacagt aaaccccgct atagttatgc
      181 tatgcatttg tctttaatga tatctcgtcg ttatattaac aggctgaaat tatcattaat
      241 gcacatttca ttattagcag ggatacctga ggagtttaaa gtgaacatat atatcgggtg
      301 gctttgtgct ggaaagttca gggcaaagcg agtatttcgt cgcgggtcat gtgctgattt
      361 ctctggcggc catatgcctg gcattattca ctaccgcatt tattatcatt tcgcagctca
      421 cgcgcggcgt taatacgttt tacaatacat tgttccccat tattggctat gcggggtcaa
      481 ttatcaccat gatatggggt tgggcactgt tagcaggcaa tgatgtgatg gcagacgagt
      541 ttgtcgccgg ccatgttatt ttcggcgttg gtatgattgc cgcctgtgta tcgacggtgg
      601 cagcgtcatc cggtcacttt ctgctcattc ccaaaaatgc agcggggagc aagagcgatg
      661 gaacgccggt acaggcttat tcttcattaa tcggtaattg cctcattgcc gttcccgttt
      721 tactcaccct gctcggtttc atttggtcta ttacgctgtt acgtagtgct gacataactc
      781 cgcattatgt cgcgggtcac gtattgcttg ggttaaccgc aatctgtgcc tgtctaattg
      841 gccttgttgc cacaattgtc catcaaacac gtaatacgtt ttcaactaaa gaacactggc
      901 tgtggtgtta ttgggttatt tttctcggct caatcacggt actgcagggg atatacgtct
      961 tagtcagttc cgatgcaagc gcccgactgg ctcccggcat tattcttatt tgcctcggaa
     1021 tgatctgtta cagcatattc tcaaaagtct ggctactggc actggtctgg agacgtacct
     1081 gttcgttagc caacagaata ccgatgatac ccgtcttcac ctgcctgttt tgccttttcc
     1141 tggcatcatt tcttgcggaa atggcgcaga ccgacatggg atattttatt ccttcgcgag
     1201 ttctggtcgg tttgggagcg gtatgcttta cgttgttctc aatcgtttca atattagaag
     1261 cgggttctgc taaaaaataa ttgcaacgta ccggataaaa ccagcgttga ccatttgcgt
     1321 aacgctggtt tttcttaggc atcatgaaat aacgcagcat taatgcatag tggttaagga
     1381 taaaaaaaca acaaagttac tgtctttttc agcctgttca tattttataa agatctggcc
     1441 actgggtgag ttttcaatca cctttccttc caccttatat taagcctgga gggtttcagt
     1501 tcacgggctc attagaaaat aatcacaaga tagccactat caaaattagc tcatttttaa
     1561 tgcggctcta ataattttca tctttaggta acaggtcagg acgacttcgc tgcaatcaaa
     1621 tcctgcgcac aagcccacgc actcgaccac gcccactgga agttatagcc ccccagccag
     1681 ccggtaacgt ccatcacttc gccgatgaag tacagcccag gcactttgcg cgcttccatc
     1741 gtccgtgaag agagttcgtt ggtgtccacg ccgccgagcg tcacttcggc agtgcgatag
     1801 ccttcagtgc cgttgggttg tacacgccag tcggtcaatg tgctaatcag tgcctgttgg
     1861 tcacgcacgt tgagctgttt tagcgaaaca ttcgggattt gcccgagttg ctgtaagcgt
     1921 tcaaccaacc gcttcggtag atgaaccgcc agtgtgtttt tcaggctctg attcggatgt
     1981 gcgttacgct gctcattcag gaaggtttcg agatccacat ccggtagcag attgatactg
     2041 acaaattcac ccggttgcca gtagctggaa atctgcaaca ccgccggtcc agacaagccg
     2101 cgatgggtga agagtaagtt ctcacggaaa acgatgccgt tttcagcggt aatcacggaa
     2161 ggcaccgcca cgcccgccag cacctgtaac tcttcgagca acggtttatg cagagtgaat
     2221 ggcaccagac ccgcacgggt cggcagcacg ttgaggccaa attgttcggc aatcttataa
     2281 ccaaacggcg acgcgcccag ccccggcatt gacagcccac cggtcgccat gaccagcttt
     2341 tcgcaaccga cagtcatgcc gttcagatca agcgtgaagc ctgtttcatc ctccgccaca
     2401 ctcagcactt cgctacgcaa tctgaaggtc acattgccct tctcgcactc atccaccagc
     2461 atgtcgacaa tctgctgcgc ggagtcatcg cagaagagtt gccctaacgt tttctcgtgc
     2521 catgcgatgc cgtgtttatt gaccagatca atgaaatccc actgggtaaa acgtgcgagt
     2581 gcagacttac aaaaatgcgg attctggctc agataagcgc ctggttcgac ataaaggttg
     2641 gtaaagttgc agcgcccacc gccagacata aggattttgc gccctggttt tttaccatta
     2701 tcgatcagca gaacccggcg tcctgcctga cctgccagcg cagaacagaa catacccgcc
     2761 gcaccagcgc ctataataat ggcatcaaac ctttccacgt tgcgctcctc ttagaaaaaa
     2821 cgggcgtgaa ttgtaaagat tcctcagtgg tcgcaccagc atcaatatta ctaaaaggaa
     2881 gtatttgcct gaattatata agataattat tttttgagtg aaatccatac aggaggcaaa
     2941 tcaaaaaaag tctatatttc actttgcccg cgccgcgaaa gtcactgata atgcgccgcg
     3001 ttcatgtcct caaaatggcg taacgtccta tgctacattt gtttgctggc ctggatttgc
     3061 ataccgggct gttattattg cttgcactgg cttttgtgct gttctacgaa gccatcaatg
     3121 gtttccatga cacagccaac gccgtggcaa ccgttatcta tacccgcgcg atgcgttctc
     3181 agctcgccgt ggttatggcg gcggtgttca actttttggg tgttttgctg ggtggtctga
     3241 gtgttgccta tgccattgtg catatgctgc cgacggatct gctgcttaat atgggatcgt
     3301 ctcatggcct tgccatggtg ttctctatgt tgctggcggc gattatctgg aacctgggta
     3361 cctggtactt tggtttacct gcatccagct ctcatacgct gattggcgcg atcatcggga
     3421 ttggtttaac caatgcgttg atgaccggga cgtcagtggt ggatgcactc aatatcccga
     3481 aagtattaag tattttcggt tctctgatcg tttcccctat tgtcggcctg gtgtttgctg
     3541 gcggtctgat tttcttgctg cgtcgctact ggagcggcac caagaaacgc gcccgtatcc
     3601 acctgacccc agcggagcgt gaaaagaaag acggcaagaa aaagccgccg ttctggacac
     3661 gtatcgcgct gatcctttcc gctatcggcg tggcgttttc gcacggcgcg aacgatggtc
     3721 agaaaggcat tggtctggtt atgttggtat tgattggcgt cgcgccagca ggcttcgtgg
     3781 tgaacatgaa tgccactggc tacgaaatca cccgtacccg tgatgccatc aacaacgtcg
     3841 aagcttactt tgagcagcat cctgcgctgc tcaaacaggc taccggtgct gatcagttag
     3901 taccggctcc ggaagctggc gcaacgcaac ctgcggagtt ccactgccat ccgtcgaata
     3961 ccattaacgc gctcaaccgc ctgaaaggta tgttgaccac cgatgtggaa agctacgaca
     4021 agctgtcgct tgatcaacgt agccagatgc gccgcattat gctgtgcgtt tctgacacta
     4081 tcgacaaagt ggtgaagatg cctggcgtga gtgctgacga tcagcgcctg ttgaagaaac
     4141 tgaagtccga catgcttagc accatcgagt atgcaccggt gtggatcatc atggcggtcg
     4201 cgctggcgtt aggtatcggt acgatgattg gctggcgccg tgtggcaacg actatcggtg
     4261 agaaaatcgg taagaaaggc atgacctacg ctcaggggat gtctgcccag atgacggcgg
     4321 cagtgtctat cggcctggcg agttataccg ggatgccggt ttccactact cacgtactct
     4381 cctcttctgt cgcggggacg atggtggtag atggcggcgg cttacagcgt aaaaccgtaa
     4441 ccagtattct gatggcctgg gtgtttaccc ttccggctgc ggtactgctt tccggcgggc
     4501 tgtactggct ctccttgcag ttcctgtaat cgtacgcacc aaaacgagcg ggtcagctgg
     4561 cccgcttcag attgtgacat agtgcgcttt gttcatgccg gatgcggcgt gaacgcctta
     4621 tccggcctac aaaaacatgc aaattcaata gattgcagag attatgtagg cctgataagc
     4681 gtagcgcatc aggcaatttt gcgtttgtaa tcagtctcga gcgggtcagt ttactggccc
     4741 gcttttttta tgcgttatta ttgctgaagt gcttaatgcc aaatcatcaa tgcaatcagg
     4801 ctgaccacca ccagaccaca caatgcgctg gtcagaataa actgccgacg cacccgctca
     4861 cagcggcgaa taaattcatc atcgtgatga tcgcgataac gttgggcata gatataccaa
     4921 acgagacgca cctgtttgtt gggttggcca tgtgaggtaa aaaagccccc tccatcaaca
     4981 tattgataga gcaatggatc gcagttacgc agtaccacta acaacgcgcg tagtgatgag
     5041 aaatagcgcg ccatgttaac aatgcaaacg acacataaag cccaaaataa tgcgacggtg
     5101 cttatcatac ctcctccccg gcgacctgcc cgcggagttc caccccgggg ctaccgctcc
     5161 cgatacgctg ccaatcagtt aacaccaggt cctggagaaa ccgcttttgt ggtgaccaac
     5221 atccgagcgg ctctatagat agtgtaggag atcaggttgt tttttttcca gaaggttaac
     5281 cactatcaat atattcatgt cgaaaatttg tttatctaac gggtaagcaa ggcggattga
     5341 cggatcatcc gggtcgctat aaggtaagga tggtcttaac actgaatctt tacggctggg
     5401 ttagccccgc gcacgtagtt cgcaggacgc gggtgacgta acggcacaag aaacgctagc
     5461 tggccagtca tcgacaactt tatggaagga gtaacactat ggcttataaa cacattctca
     5521 tcgcggtcga tctctccccg gaaagcaaag ttctggtaga gaaagcagtc tctatggctc
     5581 gcccctacaa tgcgaaagtt tctctgatcc acgtagatgt aaactactct gacctataca
     5641 ccgggcttat tgatgtgaat ctgggtgata tgcagaaacg catctctgaa gagacacatc
     5701 atgcgctgac cgagctttcc actaatgcag gctacccaat cactgaaacc ctgagcggca
     5761 gcggcgacct gggccaggtt ctggtcgatg caatcaagaa atacgatatg gatctggtgg
     5821 tttgtggtca ccaccaggac ttctggagca aactaatgtc ttccgcacgt cagctgatca
     5881 acaccgttca cgttgatatg ctgattgttc cgctgcgcga cgaagaagaa taatcatccc
     5941 cccacgacgt gttcctgaac gcccgcatat gcgggcgttt tgctttttgg cgcgccttgt
     6001 tacctgatgc agcgtaaacc ccttatctgg cctacgatct gcgtacgcaa tcaaaatctc
     6061 ctgccaatac aacatttaac actatcatat tttccatcat tagtgtgatc agctggttat
     6121 tttctgttgt aatagtgtat taatctattc accgcatcaa tattaagaat ctctgacaga
     6181 tgtaaacttt ttcgcgcgtt atcccttacg cgttcatact tttcaggatg gtattggaag
     6241 gttaataaat atgaatacaa caacacccat ggggatgctg cagcaacctc gcccattttt
     6301 catgatcttt tttgtcgagt tatgggagcg attcggctac tacggcgtgc agggcgtact
     6361 ggcggttttc ttcgttaaac agcttggatt ctcgcaagag caggcttttg tcacttttgg
     6421 tgcttttgct gcgctggtct atggcctcat ttccattggc ggctatgtcg gcgaccacct
     6481 gctggggacc aaacgcacca ttgttctcgg agcacttgtg ctggcgattg gctacttcat
     6541 gaccggcatg tcgctactta agcctgacct gattttcatc gccctgggga ctatcgctgt
     6601 cggtaacggc ctgtttaaag ctaacccagc cagcttgctt tcgaagtgct atccgccgaa
     6661 agatccgcgg cttgatggcg cattcaccct gttctatatg tcgatcaata tcggctcgtt
     6721 gatagcgtta tcgctggccc ctgtgatcgc tgatagatac ggttattcag tcacctacaa
     6781 cctgtgcggg gcggggttaa ttatcgcatt actggtttac atcgcctgtc gtggaatggt
     6841 gaaagacatt ggttctgaac ccgacttcaa gccgatgagc ttcagcaaac tgttgtacgt
     6901 attacttggc agcgtggtga tgatcttcgt atgtgcatgg ctgatgcaca acgtagaagt
     6961 cgccaatctg gtgctgattg ttctctccat cgtcgtcacc atcatcttct ttcgtcaggc
     7021 attcaagctg gataaaaccg ggcgcaataa aatgtttgtc gcctttgtcc tgatgctcga
     7081 agcggtggtg ttttacattc tctacgccca gatgccaacg tcgctgaact tctttgccat
     7141 caacaacgtg catcatgaaa ttctcggctt ttccataaac ccggtcagct tccaggcgct
     7201 taacccgttc tgggtggtac tcgccagccc aatactggca ggcatttaca cgcatctggg
     7261 taacaaaggc aaagacctct cgatgccgat gaaatttact ctcggcatgt ttatgtgctc
     7321 actgggcttt ttgacggcgg cagctgcggg aatgtggttt gcggatgcac aagggctgac
     7381 atcgccatgg tttatcgtgc tggtgtattt attccagagc ctgggtgaat tgtttattag
     7441 cgcccttggc ctggcgatga ttgctgccct ggtgccgcag catttgatgg gctttattct
     7501 cgggatgtgg ttcctgacgc aggctgccgc attcttgctg ggcggctatg tggcaacatt
     7561 taccgcagtg ccggacaaca ttaccgatcc gcttgagacg ttacccgtct ataccaacgt
     7621 gtttggtaag attggcctgg ttacgctggg cgttgcagta gtgatgctgt tgatggtgcc
     7681 gtggctgaaa cgcatgattg cgacgccgga aagccattaa ttattcttgc agaaagcagg
     7741 gtagcgttat cgctaccctg ctttactttt acacgggcgt acctgcatag atatcaaagc
     7801 gatgcccttt agtcacaacc gcgtttggcg tggcgacatt cgctagcggt ggcgcgtagt
     7861 ccgggcgctt tactaccacg cgtttggtcg ccagcaagcg tgcaggctcc agcaatccat
     7921 cggcatcaag atccggcccc accagcgact gaaagacacg catctctttt ttcaccagcg
     7981 cgcttttctg cttatgcggg aacatcgggt cgagatagac cacctgcggg cgtggggtaa
     8041 tatcggttag cgccgtcagg ctggaggcgt gaattaactg caaacgctcc tgcaaccagc
     8101 cgccgatttc cgcatccgca taaccacgcg ccaggccgtc gtcgagcagc gcggcaacaa
     8161 ctggattacg ctccagcatc cgcacgcggc aaccgactga agccagcaca aaggcatcgc
     8221 gccccagtcc tgcagtggca tccaccacat ccggcaaata atcgccttta atgcccaccg
     8281 ctttcgccac cgcctcaccg cgaccaccgc cgaatttgcg tcggtgcgcc atcgctccgc
     8341 caacaaaatc aacaaagatg ccgccaagtt ttggctcatc acgcttgcgc aattccagat
     8401 gttccggcgt taacaccagc gccatcaggt tgtcttcatc gtgctccagc ccccagcggg
     8461 ccgccagaac agataaggcg ccgtctccgg cgcctgtttc atcaattaag cagattttca
     8521 ctgaatgatc agcccttaat gccgtaatgc tccagcatcg catccagctg cggttcacga
     8581 ccacggaagc gtttgaacag atccatcggc tcttctgaac cgccacggct cagaatgttg
     8641 tcgaggaacg actgcccggt ttcacggttg aaaatgccct cttcctcaaa gcgcgagaaa
     8701 gcatctgccg ccagtacgtc agcccacagg tagctgtagt aacctgccgc ataaccaccg
     8761 gcgaaaatat ggctgaaagc gtgcgggaaa cggccccagg acggagatgg caccacggca
     8821 accagtttct tgatttctgc cagagtttcg aggatttttg ccccctgatc cgggcggaac
     8881 tcggcatgaa ggcggaaatc aaacaggccg aactccagct gacgcagaat aaacagcgcc
     8941 gcctggtagt tcttcgccgc cagcatttta tccagcaact ctttcggcag cggttcgccg
     9001 gtttcatagt gaccagagat aaacgccagc gcctccggct cccagcacca gttttccata
     9061 aactgactcg gcagttcgac cgcatcccac ggcacaccgc tgataccgga aacaccagcg
     9121 gtttcgatgc gggtcagcat atggtgcagg ccgtgaccga actcgtggaa cagggtgatc
     9181 acttcgtcgt gagtgaacag cgccggttta ccatttaccg ggcggttgaa gttacaagtc
     9241 aaatacgcga ccggtttttg cagagaacca tcagctttac gcatctggcc tacgcagtca
     9301 tccatccacg ccccgccgcg cttgttttca cgggcataca gatcgaggta gaagctgccg
     9361 cgcagttcgt tattttcgtc atacagttcg aagaaacgta catccggatg ccagacatca
     9421 acctctttac gctctttagc ggtgatgccg taaatacgtt tcaccacttc aaacaggccg
     9481 ttaaccgatt tgttttccgg gaagtacgga cgcagctgct cgtcgctgat gctgtagagg
     9541 tgctgtttct gtttttcgct gtagtaagcg atatcccacg gctgcaactc atcgacacca
     9601 aattcggctt tagcgaaggc acgcagttgc tccagctctt tttcgccttg cggacgcgcg
     9661 cgttttgcca gatcggttaa gaaatccagc acctgctgcg ggttttctgc cattttagtg
     9721 gcaagggatt taaaggcgta gttttcaaag cccagcagtt gcgccagttc gtgacgcagc
     9781 gcgaggatct cttccatcac cttgctgtta tcccacttac cggcgttcgg gccttgatcg
     9841 gaggcgcggg tgctgtaagc gcgatacatc tcttcgcgca gagcctggtt gtcgcagtag
     9901 gtcattaccg gcaggtagct tgggatatcc agcgtcagca ggtagccttc cagctctttc
     9961 gcttcggcct gggcttttgc cgcagccagc gcgctttctg gcatccccgc cagccccgct
    10021 tcgtcggtaa cgagtttggt ccagcccatc gtcgcatcga ggacgttgtt gctgtactgg
    10081 ttgcccagtt cagaaagacg ggtagcaatt tcgccgtaac gctgctgttt ctctttcggc
    10141 agacctatgc cagagagttc aaaatcacgc agggcgttat caaccgcttt cttctgcgcc
    10201 gtgttcagcg tggcgtaatg atcgccatcg cgcaggtcgc ggtacgcttt atacagccct
    10261 tcatgttgcc ctacccaggt gctgtattcc gacagcagcg gcagggtttg ctcgtaggct
    10321 tcacgcagtt ccgggctatt tttcaccgag ttcaggtggc tgaccgggga gaagatacgc
    10381 cccaacacat cgtccacttc cgccaacggc tggcagagat tttcccaggt gtacggtgcc
    10441 ccttgcgcta ctacgcgcgc cacattttcg cggcaatcgt tcagcgcctg agtcacggct
    10501 ggaacgacat gttccgggag aattttagaa aacggaggca attcaaaggg agtcagtaac
    10561 ggattcgtca ttcgcgcagt cctggttaaa gaggttaagg aagcgctcaa caggcgcttt
    10621 acataatgtg tgtagcatgg ggttaagtgt agtgaatttc aatgagaaac gttacgcttt
    10681 cgcggcggcg gcctcttttc ggtatactgt cctgatacgc ttttgtgcgc cccgaatacg
    10741 ggccgatttt tacttaccgg aacaccttta cccatgctca gttatcgcca cagctttcac
    10801 gctggcaacc acgccgacgt ccttaaacat accgttcaga gcctgatcat cgagtcgctg
    10861 aaagagaaag ataaaccgtt tctctatctc gatacccacg caggggccgg gcgttatcag
    10921 ttaggcagcg aacatgccga gcgtaccggc gaatatctcg aaggtatcgc ccgtatctgg
    10981 cagcaggacg atttgcccgc agaactggag gcgtacatca atgtggtaaa acacttcaac
    11041 cgtagcggtc agttgcgtta ctaccccggg tcgccgttga ttgctcgcca gctactgcgc
    11101 gaacaagaca gcctgcaact gaccgaactg cacccgagcg attacccgtt gctgcgttct
    11161 gaatttcaga aagacagccg tgcgcgcgtc gaaaaagccg acggtttcca gcagcttaag
    11221 gccaaacttc cgccggtttc ccgccgtggt ttaatcctta tcgacccgcc gtatgaaatg
    11281 aaaaccgact atcaggcggt ggtcagcggg atagcagaag gttacaaacg tttcgccact
    11341 ggtacttacg cactgtggta tccggtggtg ctgcgtcagc aaattaagcg catgatccac
    11401 gatctggaag cgaccggtat tcgcaaaatt ctgcaaattg aactggcggt actgccagac
    11461 agcgatcgcc gtggcatgac cgcttccggc atgattgtga ttaacccgcc gtggaaactg
    11521 gaacagcaga tgaataacgt gctgccgtgg ctgcacagca aactggttcc ggcaggcacc
    11581 gggcacgcca ccgtaagctg gatcgtgccg gagtaattgc agccattgct ggcacctatt
    11641 acgtctcgcg ctacaatcgc ggtaatcaac gataaggaca ctctgtcatg actaaacact
    11701 atgattacat cgccatcggc ggcggcagcg gcggtatcgc ctccatcaac cgcgcggcta
    11761 tgtacggcca gaaatgcgca ctgattgaag ccaaagagct gggcggcacc tgcgtgaacg
    11821 tcggctgcgt gccgaaaaaa gtgatgtggc acgcggcgca aatccgtgaa gcgatccata
    11881 tgtacggccc tgattatggt ttcgatacca ccatcaacaa gttcaactgg gaaacgttga
    11941 tcgccagccg taccgcctat atcgaccgta ttcatacttc ctatgaaaac gtgctcggta
    12001 aaaataacgt tgatgtaatc aaaggctttg cccgcttcgt tgatgccaaa acgctggagg
    12061 taaacggcga aaccatcacg gccgatcata ttctgatcgc cacaggcggt cgtccgagcc
    12121 acccggatat tccgggcgtg gaatacggta ttgattctga tggcttcttc gcccttcctg
    12181 ctttgccaga gcgcgtggcg gttgttggcg cgggttacat cgccgttgag ctggcgggcg
    12241 tgattaacgg cctcggcgcg aaaacgcatc tgtttgtgcg taaacatgcg ccgctgcgca
    12301 gcttcgaccc gatgatttcc gaaacgctgg tcgaagtgat gaacgccgaa ggcccgcagc
    12361 tgcacaccaa cgccatcccg aaagcggtag tgaaaaatgc cgatggtagc ctgacgctgg
    12421 agctggaaga tggtcgcagt gaaacggtgg attgcctgat ttgggcgatt ggtcgcgagc
    12481 ctgccaatga caacatcaac ctagaagccg ctggcgttaa aactaacgaa aaaggctata
    12541 tcgtcgtcga taaatatcaa aacaccaata ttgaaggtat ttacgcggtg ggcgataaca
    12601 cgggtgcagt ggaactgaca ccggtagcag ttgcagcggg tcgccgtctc tctgaacgcc
    12661 tgtttaataa caagccggat gagcatctgg attacagcaa cattccgacc gtggtcttca
    12721 gccatccgcc gattggtact gttggtttaa cggaaccgca ggcgcgcgag cagtacggcg
    12781 acgatcaggt gaaagtgtat aaatcctctt tcaccgcgat gtataccgcc gtcaccactc
    12841 accgccagcc gtgccgcatg aagctggtgt gcgttgggcc agaagagaag attgtcggta
    12901 ttcacggcat tggctttggt atggacgaaa tgttgcaggg cttcgcggtg gcgctgaaga
    12961 tgggcgcaac caaaaaagac ttcgacaata ccgtcgccat tcacccaacg gcggcagaag
    13021 agttcgtgac catgcgctaa atgttaaagg gctaagagta gtgtgctctt agcccttaat
    13081 tacgtttccg ctatcagttc agaagctgaa gcagaaagcg gatcagttcc agcagcgcaa
    13141 ttaacgctcc aagaacgatg attgctttat caatcacccg ttttctccat gcgatggagt
    13201 gagaatgcat ccgcttactc atccactgcc tgtcacggcg catgtctcat tgttagatga
    13261 cgactatctc actccggcca gagcatcagt taacggcacc acccgtactt ctgaccagga
    13321 ctttgaaagc gtttatgcgc attaccagag tgaaaatgcc tcagagctaa ctggataatc
    13381 atacagtaca ttcaggttat aaaaccagca tgtccttgca atagtttcag tatggtatta
    13441 gcattgatgc gttagatgat gactatctca ctccagccag agccaccaac tcagggctga
    13501 agagtaaaaa aaccgacgca aagtcggttt ttttacatcc ggattcggac aaggcttaat
    13561 atgacgatga cccagtgaaa gtatataaat cgtcattgcg atatataccg aagtgccccc
    13621 tccgccagct gaagaaatcg ctaattcttg caatgttagc cactggctaa tagtattgaa
    13681 ccgttagatg tgggcattct cactccagcc agagccacca actcaggctg aagagtaaaa
    13741 aaccgacgca aagtcggttt ttttacgtcc tgattcaaac cgcctttcaa atgaatagcc
    13801 aactcaaaat tcacacctat taccttcctc tgcacttaca cattcgttaa gtcatatatg
    13861 tttttgactt atccgcttcg aagagagaca ctacctgcca caatcaggag cgcaatatgt
    13921 catttctgtt acccatccaa ttgttcaaaa ttcttgctga tgaaacccgt ctgggcatcg
    13981 ttttactgct cagcgagctg ggagagttat gcgtctgcga tctttgtact gccctcgatc
    14041 agtcgcagcc caagatctcc cgccacctgg cattgcttcg tgaaagcggg ctattgctgg
    14101 accgcaagca aggtaagtgg gttcattacc gcttatcacc gcatattcca gcatgggcgg
    14161 cgaaaattat tgatgaggcc tggcgatgtg aacaggaaaa ggttcaggcg attgtccgca
    14221 acctggctcg acaaaactgt tccggggaca gtaagaacat ttgcagttaa aaatttagct
    14281 aaacacatat gaattttcag atgtgtttta tccgggaggc attatgttac tggcaggcgc
    14341 tatctttgtc ctgaccatcg tattggttat ctggcagccg aaaggtttag gcatcggctg
    14401 gagtgcaacg ctcggcgcag tactggcgtt agttacgggc gtggttcatc cgggtgatat
    14461 tccggtggtg tggaatatcg tctggaacgc gacggctgcg tttatcgccg tcattatcat
    14521 cagcctgctg ctggatgagt ccggcttttt tgaatgggcg gcgctgcacg tctcacgctg
    14581 gggtaatggt cgtggtcgct tgctgtttac ctggattgtc ctgctcggtg ctgccgttgc
    14641 cgccctgttt gccaatgatg gcgcggcgct tattttgacg ccgattgtca tcgccatgct
    14701 gctggcttta ggattcagta aaggcacgac gctggcgttc gtgatggcgg ccggattcat
    14761 tgccgatacc gccagcctgc cgcttattgt ctccaacctg gtgaatatcg tttccgctga
    14821 tttctttggc ctcggctttc gcgaatacgc ctcggtgatg gtgccggtgg atatcgccgc
    14881 gattgttgcc acgctggtga tgctacatct ctattttcgc aaagatattc cgcagaacta
    14941 cgatatggcg ctgctgaaat ctcccgcaga agcgatcaaa gatcctgcta cgttcaaaac
    15001 tggctgggtt gttttactgc ttctgctggt gggatttttc gtcctggaac cgctcggcat
    15061 tccggtgagc gccattgcag ctgtgggcgc gctgatatta tttgtcgtcg ctaaacgcgg
    15121 tcatgcgatt aatacgggta aagtcctgcg cggtgccccc tggcagattg tcatcttctc
    15181 gctcggcatg tatctggtgg tttatggcct gcgcaatgcc ggattaacgg aatatctttc
    15241 tggcgtactc aacgtgctgg cggataacgg cctgtgggcc gcgacgctcg gcaccggatt
    15301 cctcaccgcc ttcctctctt ctattatgaa caatatgccg acggtactgg ttggcgcgtt
    15361 gtccattgat ggcagcacgg catctggcgt tatcaaagaa gcgatggttt atgccaatgt
    15421 gattggctgc gatttgggac cgaaaattac cccaattggt agcctggcta cgctactctg
    15481 gctgcacgta ctttcgcaga agaatatgac tatcagctgg ggatattact tccgtacagg
    15541 gattatcatg accctgcctg tgctgtttgt gacgctggct gcgctggcgc tacgtctctc
    15601 tttcactttg taatgagata ctgatatgag caacattacc atttatcaca acccggcctg
    15661 cggcacgtcg cgtaatacgc tggagatgat ccgcaacagc ggcacagaac cgaccattat
    15721 ccattatctg gaaactccgc caacgcgcga tgaactggtc aaactcattg ccgatatggg
    15781 gatttccgta cgcgcgctgc tgcgtaaaaa cgtcgaaccg tatgaggagc tgggccttgc
    15841 ggaagataaa tttactgacg atcggttaat cgactttatg cttcagcacc cgattctgat
    15901 taatcgcccg attgtggtga cgccgctggg aactcgcctg tgccgccctt cagaagtggt
    15961 gctggaaatt ctgccagatg cgcaaaaagg cgcattctcc aaggaagatg gcgagaaagt
    16021 ggttgatgaa gcgggtaagc gcctgaaata aagcggcgat atccactgcc gatagttggt
    16081 aaagtgcgct ttgtttatgt cggatgcgac gctaacgcgt cttatccgac ctacaaaacc
    16141 gtgcaaattc aatatatggc atgaaatatg tagagcgtag cgcatcaggc aattttgcta
    16201 tttacgcagc actcctcgcg taagaattca attatcgctt ccagcattgc gtattgcgaa
    16261 acacacatca gcgtccgact ctcacggttt tgtttgacca gtcctaccga taccagtgct
    16321 gatatatggt ggctgagtgt ggagccaggt atcccaagct gcttttgcaa ctcaccgacc
    16381 ggcaaccctt gttccccggc tttgacaaga tgtttaaata tgaacaaacg ggtcgggtga
    16441 cctagctctt tcagcgcttt agcgacttct tccaactgca tatgacaccc ctctgtctgg
    16501 ttgatatttc gataatacca gaaatatctt gatctgaatt tcatttcgat tattctggaa
    16561 atatagattt atccaatgag gttacgtatg agtagttggc ttgcgatgct gcaagatgcc
    16621 gcagagatgt ttgtgtttct cgccgtcgag ctttcgttgc tgtttatagt gattagtgcc
    16681 ggtgtcagcc tgataagaca aaaggtgcca gaccataaaa tccagcagat gatgggggcc
    16741 agaaaaggga gaggctatct cctggctgct ctgttgggag ccgttacccc gttctgtagt
    16801 tgctcgacaa tacccatgtt acgtggattg ttgtcagcga aagccgggt
//
LOCUS       DAZPAC010000079        16569 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZPAC010000079 DAZPAC010000000
VERSION     DAZPAC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16569
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.079"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHUPM_002165"
     CDS             complement(<1..170)
                     /locus_tag="ACHUPM_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HFR0869938.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="ACHUPM_002166"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="ACHUPM_002166"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR0869939.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="ACHUPM_002167"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="ACHUPM_002167"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR0869940.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="ACHUPM_002168"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="ACHUPM_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR0869941.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHUPM_002169"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHUPM_002169"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR0869942.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHUPM_002170"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHUPM_002170"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR0869943.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="ACHUPM_002171"
     CDS             4288..5151
                     /locus_tag="ACHUPM_002171"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR0869944.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="ACHUPM_002172"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="ACHUPM_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR0869945.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="ACHUPM_002173"
     CDS             6489..7106
                     /locus_tag="ACHUPM_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR0869946.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="ACHUPM_002174"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="ACHUPM_002174"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR0869947.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="ACHUPM_002175"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="ACHUPM_002175"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR0869948.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="ACHUPM_002176"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="ACHUPM_002176"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR0869949.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="ACHUPM_002177"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="ACHUPM_002177"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR0869950.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="ACHUPM_002178"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="ACHUPM_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR0869951.1"
                     /translation="MNPTRYARICEMLARWQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="ACHUPM_002179"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="ACHUPM_002179"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR0869952.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="ACHUPM_002180"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="ACHUPM_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR0869953.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
BASE COUNT         4097 a         4496 c         4198 g         3778 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 tctacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgatgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggtt
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttacgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaattt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcctg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggtggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggta tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg
    16501 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    16561 tgtctgttt
//
LOCUS       DAZPAC010000080        16521 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZPAC010000080 DAZPAC010000000
VERSION     DAZPAC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16521
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.080"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            117..1763
                     /gene="mqo"
                     /locus_tag="ACHUPM_002181"
     CDS             117..1763
                     /gene="mqo"
                     /locus_tag="ACHUPM_002181"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFR0869954.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(2072..2581)
                     /gene="eco"
                     /locus_tag="ACHUPM_002182"
     CDS             complement(2072..2581)
                     /gene="eco"
                     /locus_tag="ACHUPM_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR0869955.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(2695..2859)
                     /gene="yojO"
                     /locus_tag="ACHUPM_002183"
     CDS             complement(2695..2859)
                     /gene="yojO"
                     /locus_tag="ACHUPM_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR0869956.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            2968..3462
                     /gene="napF"
                     /locus_tag="ACHUPM_002184"
     CDS             2968..3462
                     /gene="napF"
                     /locus_tag="ACHUPM_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR0869957.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            3452..3715
                     /gene="napD"
                     /locus_tag="ACHUPM_002185"
     CDS             3452..3715
                     /gene="napD"
                     /locus_tag="ACHUPM_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR0869958.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            3712..6198
                     /gene="napA"
                     /locus_tag="ACHUPM_002186"
     CDS             3712..6198
                     /gene="napA"
                     /locus_tag="ACHUPM_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR0869959.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            6205..6900
                     /gene="napG"
                     /locus_tag="ACHUPM_002187"
     CDS             6205..6900
                     /gene="napG"
                     /locus_tag="ACHUPM_002187"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR0869960.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            6887..7750
                     /gene="napH"
                     /locus_tag="ACHUPM_002188"
     CDS             6887..7750
                     /gene="napH"
                     /locus_tag="ACHUPM_002188"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR0869961.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            7747..8196
                     /gene="napB"
                     /locus_tag="ACHUPM_002189"
     CDS             7747..8196
                     /gene="napB"
                     /locus_tag="ACHUPM_002189"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR0869962.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            8206..8808
                     /gene="napC"
                     /locus_tag="ACHUPM_002190"
     CDS             8206..8808
                     /gene="napC"
                     /locus_tag="ACHUPM_002190"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR0869963.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            8821..9444
                     /gene="ccmA"
                     /locus_tag="ACHUPM_002191"
     CDS             8821..9444
                     /gene="ccmA"
                     /locus_tag="ACHUPM_002191"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR0869964.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            9441..10103
                     /gene="ccmB"
                     /locus_tag="ACHUPM_002192"
     CDS             9441..10103
                     /gene="ccmB"
                     /locus_tag="ACHUPM_002192"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR0869965.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            10145..10882
                     /gene="ccmC"
                     /locus_tag="ACHUPM_002193"
     CDS             10145..10882
                     /gene="ccmC"
                     /locus_tag="ACHUPM_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR0869966.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            10879..11088
                     /gene="ccmD"
                     /locus_tag="ACHUPM_002194"
     CDS             10879..11088
                     /gene="ccmD"
                     /locus_tag="ACHUPM_002194"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR0869967.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            11085..11564
                     /gene="ccmE"
                     /locus_tag="ACHUPM_002195"
     CDS             11085..11564
                     /gene="ccmE"
                     /locus_tag="ACHUPM_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR0869968.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            11561..13504
                     /gene="ccmF"
                     /locus_tag="ACHUPM_002196"
     CDS             11561..13504
                     /gene="ccmF"
                     /locus_tag="ACHUPM_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR0869969.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            13501..14058
                     /gene="dsbE"
                     /locus_tag="ACHUPM_002197"
     CDS             13501..14058
                     /gene="dsbE"
                     /locus_tag="ACHUPM_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR0869970.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            14055..15107
                     /gene="ccmH"
                     /locus_tag="ACHUPM_002198"
     CDS             14055..15107
                     /gene="ccmH"
                     /locus_tag="ACHUPM_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR0869971.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(15142..15789)
                     /gene="narP"
                     /locus_tag="ACHUPM_002199"
     CDS             complement(15142..15789)
                     /gene="narP"
                     /locus_tag="ACHUPM_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR0869972.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            16109..16480
                     /locus_tag="ACHUPM_002200"
                     /pseudo
     CDS             16109..16480
                     /locus_tag="ACHUPM_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         3611 a         4143 c         4786 g         3981 t
ORIGIN      
        1 tctaaaatta gaatataatt tataaacatt atttaaatgt tgttacttta gtgttaccgt
       61 tgatgccgcg caaatctcac ctgcaataaa gcgactaaaa gtaaggcatt aacaagatga
      121 aaaaagtgac tgccatgctc ttctcgatgg ccgtggggct taatgccgtt tcgatggcgg
      181 caaaagcgaa agcgtccgag gagcaggaaa ctgatgtact gttgattggc ggcggcatta
      241 tgagcgccac gttggggacc tatttacgcg agctggagcc tgaatggtcg atgaccatgg
      301 tggagcgcct ggagggtgtc gcgcaggaga gttcgaacgg ctggaacaac gccggaaccg
      361 ggcattctgc actgatggaa ctgaactaca ccccgcaaaa cgccgatggc agtatcagta
      421 ttgaaaaagc agtcgccatt aacgaagcat ttcagatttc ccgccagttc tgggcgcacc
      481 aggtcgagcg cggcgtgctg cgtactccgc gttcatttat caataccgtt ccgcatatga
      541 gctttgtctg gggcgaggat aacgttaatt tcctgcgcgc ccgttacgcc gcgttgcaac
      601 aaagctcgct gtttcgcggt atgcgttact ctgaagatca cgcgcagatc aaagagtggg
      661 caccgttagt gatggaaggg cgcgatccgc aacagaaagt ggcagccacg cgtacggaaa
      721 ttggtaccga tgtgaactac ggcgagatca cccgccagtt aattgcttcc ttgcagaaga
      781 aatctaactt ctcgctgcaa ctcagcagcg aagtccgcgc cctaaagcgt aatgacgata
      841 acacctggac cgttaccgtt gccgatctga aaaatggcac tgcacagaac attcgtgcca
      901 aatttgtctt tatcggcgcg ggcggtgcgg cgctgaagct gttacaggaa tcggggattc
      961 cggaagcgaa agactacgcc ggtttcccgg tgggcggaca gttccttgtt tcggaaaacc
     1021 cggacgtggt taatcaccat ctggcgaagg tttacggtaa agcatccgtt ggcgcaccac
     1081 cgatgtcggt tccgcatatc gatacccgcg ttctggacgg taaacgcgta gtgctgtttg
     1141 ggccatttgc caccttctca accaaattcc tcaaaaacgg ttcattgtgg gatctgatga
     1201 gttccaccac cacctctaac gtgatgccga tgatgcacgt cgggctggat aatttcgatc
     1261 tggtgaaata cttggtgagt caggtgatgt tgagtgaaga ggatcgtttt gaagcgttga
     1321 aagagtacta tccgcaggcg aaaaaagagg actggcgttt gtggcaagcg gggcagcgtg
     1381 tgcagattat caagcgtgat gccgataaag gtggtgtact gcgtctgggt actgaagtgg
     1441 tcagtgacca gcaagggacc attgccgcgc tgctgggggc atcgccaggg gcgtcaaccg
     1501 ctgcgccgat tatgttggat ctgctggaaa aagtatttgg cgatcgcgtt tccagcccgc
     1561 aatggcaggc tacgttgaaa gcgatcgttc catcttatgg acgcaagctg aacggtgatg
     1621 tagcggcaac agaacgcgag ttgcagtaca ccagcgaagt gctgggcctg aaatacgaca
     1681 agccgcaggc agcagatagt acgccgaaac cgcagttgaa accgcaaccc gttcaaaaag
     1741 aagtggcgga tattgcgttg taatgatgtg ccacatccgg catggtatgc cggatgtggc
     1801 gtaaccgcct tatccgacct acggggtgga gcacttcaca gcggtgtagg cctgataaga
     1861 cgcgtcagcg tcgcatcagg caaccggctc gggcgttaga tgtcggatgc ggcgtaaccg
     1921 ccttatccaa cctacggggt ggagcacttc acagcggtgt aggcctgata agacgcgcca
     1981 gcgtcgcatc aggcaaccgg cttgggcgtt agatgtcgga tgcggcgtga ccgccttatc
     2041 cgacctacgg ggtgacgcac ttcacagcag tttagcgaac taccgcgttg tcaattttct
     2101 cttccgcctt ccagacgcgg tacttcacat ctacattgtc tggcgtatac accacgatcg
     2161 gcagcttgct gttgtaacgc agcattccag catcgcccag atacgcggtg acaaatttct
     2221 tctctttctt gccatccggg caggccatca tcgtggaaac cggcgagcta actttatcaa
     2281 agacataata atcatagccc cagccttcca gcgttttgtt ttccagcttc ccgccgagac
     2341 gatgcaaatt gcaatcgact tccagcgtct gaccggttaa cagttctact ttcagggtag
     2401 attcatcttc ttgcggggtt aactgaatca cctgacgctt catccctttt tcagcttgtg
     2461 gataaggcgc gattttttcc agtggctgga cgctttctgc cgcccaggcg gaagtggtgg
     2521 cgaaagcggc aaacaatact gcaggtagaa tggtcttcat ttttttgctt tccttcttca
     2581 tttataggtt ttcaccgatg atgtcgctga aggttaacat attcatcaga tttattaatt
     2641 tgctttatga ataacttaaa gaacattcag acaattttac ctgctctttt agtgttaaat
     2701 tctgatgagg gagcaaacca gcagaaatat ctctggtgaa ttaatcgcta tataaatatg
     2761 tttataacca tttgaaatgt gagcaaaagc ccgtttttgc cacgcttccc gccactcttt
     2821 tgatcctgct acaggtttta ccccgatcgg ggtatgcatc tttgacacat cctttaatat
     2881 cttagcggct ataaaaatgg cttattaatt atgcggttta tttggtcgct ctcaattttc
     2941 agagcgcgtt aatgatggaa ggtcaatgtg aagattgatg catcccgtcg gggcatactc
     3001 actggtcgct ggcgcaaagc cagtaacggt atccgtccgc cctggtccgg tgatgaatct
     3061 cattttctga cccattgcac ccgttgcgac gcctgtatta atgcttgtga aaacaacatt
     3121 ctgcaacgcg gcgcgggcgg ctatccgagc gttaatttca aaaataacga gtgcagcttc
     3181 tgttacgcct gcgcgcaggc ttgccccgaa tcgctatttt ctccgcgcca caccagggcc
     3241 tgggatttgc agttcaccat tggggacgcg tgcctggcgt atcagtcagt tgaatgccgc
     3301 cgctgtcagg atagctgtga gccaatggca attatctttc gcccgacgct ctccgggatc
     3361 taccagccgc aacttaatag ccaactttgc aacggatgtg gagcatgtgc agccagctgt
     3421 ccggtatcag ccataactgc ggagtatctc catgcacact aactggcaag tttgcagcct
     3481 ggtcgtgcag gccaaaagcg aacgaatttc agacatcagc acccaactga acgcctttcc
     3541 cggctgtgaa gttgctgtca gcgacgcgcc gagcggtcag ttgattgtgg tggtggaagc
     3601 agaagacagc gaaacgctga tccaaaccat tgagtcagta cgcaacgtag agggcgtgct
     3661 ggcggtgtcg ctggtttatc accagcagga agagcaaggt gaggaaacac catgaaactc
     3721 agtcgtcgta gctttatgaa agctaacgcc gttgcggccg ctgcggcggc tgccggtctc
     3781 agcgtgccgg gcgttgcccg cgccgttgtt ggtcagcagg aagccattaa atgggataaa
     3841 gcgccgtgcc gtttctgcgg tactggttgc ggcgttctgg tcggaacgca gcaggggcgt
     3901 gtggtggcct gtcagggcga cccggacgca ccggttaacc gtggcctgaa ctgcattaag
     3961 ggctatttcc tgcccaagat catgtacggt aaagaccgtt tgacgcagcc gctgctgcgt
     4021 atgaaaaacg gtaaatatga caaagaaggc gaatttaccc caatcacctg ggatcaggcc
     4081 ttcgatgtga tggaagagaa attcaaaacc gccctgaaag aaaaagggcc ggaatcgatc
     4141 ggtatgttcg gttctggtca gtggactatc tgggaaggtt atgccgcgtc caagctgttt
     4201 aaagcgggct tccgttcgaa caacatcgac ccgaacgcgc gtcactgtat ggcgtcggca
     4261 gtagttggct ttatgcgtac ctttggtatg gatgagccga tgggctgcta tgacgacatc
     4321 gagcaggctg acgcgtttgt gctgtggggc gcaaacatgg cggagatgca cccgatcctc
     4381 tggtcgcgca tcactaaccg tcgtctctct aatcagaacg tcaccgtggc ggtgctttct
     4441 acctaccagc atcgtagctt cgagctggcg gataacggca tcatctttac gccacagtct
     4501 gacctggtga tcctgaacta catcgccaac tatatcattc aaaacaatgc gataaatcag
     4561 gacttcttca gcaaacacgt taacctgcgc aaaggggcga cggacatcgg ctacggttta
     4621 cgtccgaccc atccgctgga aaaagcagcg aagaatccag gttctgacgc ctccgaaccg
     4681 atgagctttg aagattacaa agccttcgtt gccgagtata cgctggaaaa aaccgctgaa
     4741 atgaccggcg tgccgaaaga ccagttagaa caactggcgc agctgtatgc cgatccgaac
     4801 aagaaagtca tctcctactg gacgatgggc ttcaaccagc atactcgtgg cgtgtgggct
     4861 aacaacctgg tctacaacct gcacctgctg accggcaaaa tttcccagcc gggttgcggt
     4921 ccgttctccc tgaccgggca accttctgcg tgtggtactg cgcgtgaagt gggcaccttt
     4981 gctcaccgtc tgcctgcgga catggtggtg accaacgaga aacaccgtga tatctgcgag
     5041 aagaagtgga atatcccgag cggcaccatt ccggcgaaaa tcggtctgca cgcggtggca
     5101 caagaccgtg cgctgaaaga cggcaaactg aatgtttact ggaccatgtg taccaacaac
     5161 atgcaggccg ggccgaacat caatgaagag cgtatgccgg gctggcgcga tccgcgcaac
     5221 ttcatcatcg tctccgatcc gtatccgaca gtcagtgcgc tggccgccga cttgatcctg
     5281 ccgaccgcaa tgtgggtaga gaaagagggt gcttacggta acgccgaacg ccgtactcag
     5341 ttctggcgtc agcaggtaca ggcaccgggc gaagcgaaat cggatctctg gcagttagtc
     5401 cagttctccc gccgcttcaa aactgaagaa gtgtggccgg aagagctgct agcgaagaaa
     5461 ccggaactgc gtggcaaaac gctgtacgaa gtgctttatg ccacaccgga agtgagcaaa
     5521 ttcccggtat ccgaactggc ggaagatcag ctgaacgatg aatcccgcga gctgggcttc
     5581 tatctgcaaa aagggctgtt cgaagagtac gcatggtttg gtcgcggtca cggtcacgat
     5641 ctcgcgccgt tcgatgacta ccacaaagcg cgcggtctgc gctggccggt ggtgaacggt
     5701 aaagaaacgc agtggcgtta cagcgaaggt aacgacccgt acgtgaaagc gggcgaaggc
     5761 tataagttct acggtaaacc ggatggtaaa gcggtgatct tcgcgctgcc gttcgaaccg
     5821 gcggcggaag caccggatga agagtacgac ctgtggctct ctaccggacg cgttctggag
     5881 cactggcaca ctggcagtat gactcgccgt gtgccggaac tgcaccgcgc cttcccggaa
     5941 gcggtcttgt ttattcaccc gctggatgcg aaagcgcgcg atctgcgccg tggcgacaaa
     6001 gtgaaagtgg tttctcgccg tggcgaagtg atctcgattg ttgaaacgcg cggtcgtaac
     6061 cgtccgccac agggcctggt gtacatgccg ttcttcgacg ccgcacagct ggttaacaaa
     6121 ctaacgctgg acgcgaccga tccgctctcg aaagagacgg acttcaagaa gtgcgcagtc
     6181 aaactggaga aggtgtaagc cattatgtcc cggtcagcga aacctcaaaa tggtcgccgc
     6241 cgctttctgc gcgatgttgt tcgcacagca ggcgggctgg ctgccgtggg tgtggcgctg
     6301 gggttacaac agcaaaccgc acgcgcatct ggcgtgcggt tgcgcccgcc cggagccata
     6361 aacgagaacg cctttgccag tgcctgtgtg cgttgcggtc agtgtgttca ggcttgccct
     6421 tacgacacct taaaactggc gacgctggct tctggcctgt cggcgggcac gccatatttt
     6481 gtcgcacggg atattccttg cgaaatgtgt gaggacattc cgtgcgccaa agtgtgcccg
     6541 agtggtgcgc tggatcgtga gatcgaatcg atcgacgacg cgcggatggg gctggcggtg
     6601 ctggtggacc aggaaaactg tctcaacttt caggggctgc gctgcgatgt ttgttatcgc
     6661 gaatgcccga aaattgatga ggccatcacc ctggagctgg agcgcaacac gcgtaccggt
     6721 aagcacgccc gctttctgcc gacggttcac agcgacgcct gtaccggctg cggtaagtgc
     6781 gaaaaagtgt gcgtgctgga acaaccggca atcaaggtgt taccgctgtc actggcgaaa
     6841 ggggagttag gtcaccatta ccgcttcggc tggctggagg ggaacaatgg caaatcgtaa
     6901 acgtgacgcc gggcgcgagg cgctggtgaa aaaaggctgg tggcgcagtc accgttggct
     6961 ggtgttacgt cgcctttgtc agttcttcgt gctggggatg tttttgagcg gtccgtggtt
     7021 tggtgtgtgg atcttacacg gcaactacag ctgtagcctg ttattcgaca ccgttccgct
     7081 gaccgatccg ctgatgacgc tgcaaagtct tgccagtggt catctgcctg ccacggtggc
     7141 gttgaccggt gcggtgataa tcactgtgct ctacgccctg gcaggaaagc gattattttg
     7201 cagctgggtc tgcccgctga acccgattac cgacttagcg aactggttac gcaggcgctt
     7261 tgacctcaat cagtctgcga cgatccctcg ccacattcgc tacgtgctgt tggtggtcat
     7321 tctgctggga tcagccttaa ccggcacgct catctgggaa tggatcaacc ccgtttccct
     7381 gatggggcgt agcctggtaa tgggcttcgg cagcggcgcg ctgcttattc tcgcgctgtt
     7441 tttatttgat ctactggtcg ttgaacacgg ctggtgcggg cacatttgtc cggttggcgc
     7501 gctgtatggc gtgctgggta gcaaaggtgt gattaccgtt gcggccagcg atcggcagaa
     7561 atgtaaccgc tgtatggatt gttttcatgt ttgcccggaa ccgcatgtgc tacgtgcccc
     7621 ggtgctggat gaacaaagcc cggtgcaggt caccagccgc gattgcatga cgtgcggtcg
     7681 ctgcgtggat gtctgttctg aggatgtatt tacaataact acacgatgga gttcgggagc
     7741 gaaatcatga aaagccatga cctgaagaaa gcgctgtgtc aatggacggc gatgctggcc
     7801 ctggtggtaa gcggcgcggt ttgggcggct aatggcgtcg attttagcca gtcgccggaa
     7861 gtatccggga cgcaggaagg ggccattcgg atgccaaaag agcaggatcg gatgccgctg
     7921 aactatgtga atcagccgcc aatgatcccg catagcgttg aaggttatca ggtaacgacc
     7981 aataccaacc gctgcttgca gtgccacggt gtcgaaagct atcgcaccac tggcgcgccg
     8041 cgtatcagtc ctactcactt tatggacagc gacggcaaag tgggcgcgga agtggctccg
     8101 cgtcgttatt tctgtctgca atgtcacgta ccgcaggccg ataccgcgcc aatcgtgggg
     8161 aataccttta ccccatcaaa aggttacggg aaataagagg ttattatggg aaattctgac
     8221 cgtaagcctg gtctgattaa gcgcctgtgg aaatggtggc gtacccccag ccgtctggcg
     8281 ctgggaacgc tgctgttgat cggttttgtc ggcggcatcg tcttctgggg cggctttaac
     8341 accggaatgg aaaaagccaa taccgaagag ttctgcatta gctgccacga aatgcgtaac
     8401 acggtgtatc aggaatacat ggattccgtg cactacaaca accgtagcgg cgtccgtgcg
     8461 acctgtccgg attgtcacgt tccgcacgag tttgtgccga agatgatacg caagctcaaa
     8521 gcaagtaaag agctgtatgg taaaattttt ggcgttattg acacgccgca gaaatttgaa
     8581 gctcatcgtc tgacaatggc acagaatgag tggcggcgca tgaaggacaa taactcgcag
     8641 gagtgccgta actgtcacaa cttcgagtat atggatacaa ccgcccagaa atcggttgcc
     8701 gcgaagatgc atgaccaggc ggtgaaagat gggcaaacct gtattgattg ccataaaggg
     8761 atagcgcaca agctgcccga tatgcgtgaa gtcgagccag gtttttaaca gggttattgc
     8821 gtgggtatgc ttgaagccag agagttactt tgtgagcggg atgaacgaac cttatttagt
     8881 ggcttgtcat ttacgctgaa cgcaggagag tgggtacaaa tcaccggtag caacggcgcg
     8941 gggaagacaa cgcttctccg tttgctgacg gggttgtctc gccctgacgc aggcgaggtt
     9001 ctctggcaag ggcagccctt gcatcaggta cgcgacagct accatcaaaa cctgttatgg
     9061 ataggccatc agccggggat caaaacccgg ctgacggcgt tagaaaatct gcacttttat
     9121 catcgcgatg gcgataccgc acaatgtctg gaagccctgg cgcaggccgg gcttgccgga
     9181 ttcgacgata ttccggtaaa tcaactttct gccgggcaac aacgccgcgt cgctttagcg
     9241 cgtctgtggc tgacccgtgc cacgttatgg atcctcgacg agccttttac cgcgattgac
     9301 gttaacggcg tcgatcgtct gacccagcgt atggcgcagc atacggagca gggggggatt
     9361 gtgattctga ctacccacca gccgctcaac gttgctgaaa gtaaaattcg ccgcatttca
     9421 ctgacgcaaa cgagggccgc atgatgttct ggcgcatttt ccgtcttgag ctgcgtgtag
     9481 cgtttcgcca tagcgccgaa atcgccaacc cgctgtggtt cttcctgatt gtaattaccc
     9541 tttttccgct cagtatcggt ccggagccgc aactgctggc gcgtattgca ccgggcatta
     9601 tctgggttgc tgcgctgctt tcatccttgc tggcgctgga acgactgttc cgtgacgatt
     9661 tgcaggacgg cagtcttgaa caattgatgt tgttgccgtt acccttgccc gccgttgtgc
     9721 tggcgaaggt gatggcgcac tggatggtaa ccggtctgcc gttactcatc ctttcgccac
     9781 tggtagcaat gctactggga atggatgttt atggctggca agtgatggcg ctgacgctgc
     9841 tgctgggaac gcctacgctt ggctttctcg gtgcaccggg tgtggcgttg acagtgggac
     9901 ttaagcgcgg tggtgtgctg ctcagcatac tggtgttacc gctgactatc ccattactca
     9961 tctttgccac cgccgcgatg gacgcggctt ccatgcattt gcccgttgac gggtatctgg
    10021 caattttagg cgcgttgctg gcaggcaccg cgacattaag tccttttgcg acggcggcag
    10081 cgttacgaat cagcattcaa taacgctgga ttactgattt ttgttcgagt ctggtatcga
    10141 aactatgtgg aaaacactgc atcaactggc gatcccacca cggctgtatc aaatctgtgg
    10201 ctggtttata ccgtggctgg caattgccag tgtggtcgtg cttaccgtcg gctggatctg
    10261 gggattcggc tttgctccgg ctgattatca gcagggaaat agctaccgca ttatctacct
    10321 gcatgtgcct gcggcgatct ggtcgatggg catttatgca tcaatggcag tggcagcgtt
    10381 tattggcctt gtctggcaga tgaaaatggc caacctggcg gtggcggcga tggcccccat
    10441 tggtgccgtg tttaccttta ttgccctggt taccggctct gcatggggaa aaccgatgtg
    10501 gggcacctgg tgggtatggg atgcacgtct gacttctgaa ctggtgctgc tgtttttgta
    10561 tgtgggtgtg attgccctgt ggcacgcctt cgacgaccgc cgtctggcgg gccgtgcggc
    10621 aggtatcctg gtgctgattg gcgtggtgaa tctgccgatt attcattact ccgtggagtg
    10681 gtggaacacc ctgcatcagg gatcaacgcg gatgcagcaa agtatcgatc cggcgatgcg
    10741 ttcgccgctg cgctggtcga tttttggctt cctgctcctg tctgccacgc tgacgctgat
    10801 gcggatgcgt aatttgattt tgctgatgga aaaacgccgt ccgtgggtga gtgaactgat
    10861 actgaaaaga ggccgtaaat gacccctgca tttgcttcct ggaatgaatt tttcgcaatg
    10921 ggcggttacg ccttttttgt ctggctggcg gtggtgatga ccgttattcc gctggtggtt
    10981 ttggtcgtgc actcggtgat gcaacatcgc gcaattctgc gtggtgtggc gcaacagcgg
    11041 gcgcgtgagg cgcgtttacg tgctgcgcaa cagcaggagg ctgcatgaat attcgccgta
    11101 aaaaccgctt gtggattgcc tgtgccgtgt tggcagggct ggcgctgact atcggtctgg
    11161 tgctatatgc gctgcgctcg aatatcgatc tcttttatac gccgggggaa attctctacg
    11221 gcaagcgtga aactcagcaa atgccggaag tcggtcagcg tttgcgcgtt ggcgggatgg
    11281 tgatgctggg tagtgtgcag cgcgatccca attcgctgaa agtgaccttc actatttacg
    11341 atgctgaagg ctcagtggat gtctcttacg aaggcatttt gccggatctg ttccgtgaag
    11401 ggcagggcgt tgtggtgcag ggcgagctgg aaaaaggcaa tcatatcctc gcgaaagaag
    11461 tgctggcgaa acacgacgaa aactacacgc cgccagaagt tgagaaagcg atggaagcta
    11521 accaccgtcg cccggcgagt gtttataagg acccagcatc atgatgccgg aaattggaaa
    11581 cgggctgctg tgtctggcgt tagggattgc gctgctgctg tccgtgtatc cgctatgggg
    11641 cgtggcgcgc ggagatgcgc gcatgatcgc gtcttcccgc ttgtttgcct ggctgctgtt
    11701 tatgtctgtg gctggcgcat ttctggtgct ggtcaatgct ttcgtggtca acgactttac
    11761 cgtcacctat gttgccagct actccaatac ccagcttccg gtgtggtatc gcgtggcggc
    11821 tacctggggc gcgcatgaag gctcgctact gctgtgggtg ctgctgatga gcggctggac
    11881 ctttgcggtg gcgattttta gtcagcgtat tccgctggat attgtggccc gcgtactggc
    11941 gataatgggg atggtcagcg tcggcttttt gctgttcatt ctctttacct ctaacccatt
    12001 ctcacgcacg ctgccgaact tcccgattga agggcgcgat cttaacccgc tgttacagga
    12061 cccggggctg atcttccatc cgcctctgct ttatatgggg tacgtgggtt tctcggtggc
    12121 gtttgctttt gccattgctt ctttgctgag cgggcgtctg gacagcactt atgcgcgttt
    12181 tactcgcccg tggacgctgg cggcgtggat tttcctgacg ctcggcattg tgctcggttc
    12241 cgcatgggcc tattacgaac tcggctgggg gggctggtgg ttctgggatc cggtagaaaa
    12301 cgcctcgttt atgccgtggc tggtggggac tgcgctgatg cactcactgg cggtcactga
    12361 acaacgcgcc agcttcaaag cgtggacatt actgctggca atcagtgcct tctcgttgtg
    12421 tctgttgggg actttcctcg tgcgttccgg cgtgctggta tcggtacacg cgtttgcgtc
    12481 tgatccggcg cgcggtatgt ttatcctcgc ctttatggtg ctggtgattg gaggttcgct
    12541 gctgctgttt gccgcgcgtg gacacaaagt tcgctcacgc gtaaacaatg cgctgtggtc
    12601 gcgggaatct ctgctgttag cgaacaacgt tttgctggtc gccgcgatgc tggtggtgtt
    12661 gctggggacg ctgctgccgc tggtgcacaa gcaactggga ctgggcagta tttcgattgg
    12721 cgaaccgttc ttcaacacca tgtttacctg gctgatggtg ccgtttgcgc tactgctggg
    12781 tgtcggtcct ctggtgcgct gggggcgcga tcgcccacgt aaaatccgca atttattgat
    12841 tatcgccttc atctcaacgc tggtgctgtc tctgctgttg ccgtggctgt ttgagagcaa
    12901 agttgtggcg atgacggtgc tcggcctggc aatggcctgc tggattgcgg tgctggcaat
    12961 tgcggaagct gcgctgcgta tttcacgcgg cacgaaaacc accttcagtt attgggggat
    13021 ggtggctgct cacctggggc tggcagtgac aattgttggc attgctttta gccagaacta
    13081 tagcgttgag cgtgatgtgc gcatgaagtc cggcgatagc atcgatattc atgaatatcg
    13141 cttcaccttc cgtgatgtca aagaggtgac tggcccgaac tggcgtggcg gtgtggcgac
    13201 tatcggcgta acgcgagagg gtaaaccgga aacagtgctg tatgcggaaa aacgttatta
    13261 caacactgcc gggtcgatga tgaccgaagc ggcgattgac ggtggcatca cgcgtgacct
    13321 gtacgccgcg ctcggtgaag agctggaaaa cggcgcgtgg gctgtgcgtc tttactacaa
    13381 accatttgtt cgctggattt gggcgggcgg gctgatgatg gcgttgggcg gactgctgtg
    13441 tctgtttgat cctcgctatc gtaagcgcgt gagtccgcaa aaaactgcgc cggaggctgt
    13501 atgaagcgca aagtattgtt aattccgttg attatcttcc tggcgattgc cgcggcgctg
    13561 ctgtggcagc tggcgcgtaa tgccgaaggg gatgatccga ccaatctgga atcggcgctc
    13621 attggcaagc cagtgccgaa gtttcgcctc gaatcactgg acaatccggg gcagttttat
    13681 caggcggatg tgctgactca gggcaaacca gtgctgctta acgtctgggc gacctggtgt
    13741 ccgacctgcc gtgcggaaca tcaatatctg aatcagcttt ctgcgcaggg catccgcgta
    13801 gtcggcatga actataaaga cgatcgccag aaggcaatca gctggctgaa agagctgggc
    13861 aatccttacg cgctaagcct gtttgatggc gatggcatgt tagggctgga tctcggtgtc
    13921 tatggcgcgc cagaaacgtt ccttattgac ggcaacggca tcattcgcta tcgccatgcg
    13981 ggcgatctca atcctcgcgt ctgggaagaa gagatcaagc cgctgtggga gaaatacagt
    14041 aaggaggccg cacaatgagg tttttattgg gcatgctgat gctgatgatc tccggctcag
    14101 cgctggcgac catagacgtg ttgcagttta aagatgaagc acaggaacaa cagttccgtc
    14161 agctcactga agaactgcgc tgcccgaaat gccagaacaa cagcattgcc gattccaact
    14221 cgatgattgc caccgacctg cgccagaaag tgtatgaact gatgcaggaa ggtaaaagta
    14281 aaaaagagat tgtcgattat atggtggcgc gttacggcaa cttcgtcact tacgatccgc
    14341 cgttaacgcc gctgaccgtg ctgctgtggg tgctgccagt agtagctatt ggcattggcg
    14401 gttgggtcat atacgcccgc tcgcggcgtc gggtacgcgt ggtgccggaa gcgtttcctg
    14461 aacaaagcgt gccggaaggt aagcgtgccg gatatgtcgc ttatttgccg ggcattgtgg
    14521 tggcgttaat tgtggctggc gtcagctact accagaccgg aaattatcaa caggtgaaaa
    14581 tctggcagca ggccacggca caggctccgg cgttgctgga cagggcgctg gatccgaaag
    14641 ctgatccgct caacgaagaa gagatgtcgc gccttgcgct ggggatgcgt actcaactgc
    14701 aaaaaaatcc gggagatata gacggctgga ttatgttggg ccgcgttggc atggcgctgg
    14761 gtaacgccag tatcgccacc gatgcatacg ctactgcgta tcgcctcgat ccgaaaaaca
    14821 gtgatgccgc actgggttat gctgaagcgt tgacacgttc atctgatccc aacgacaacc
    14881 gcctcggcgg cgaactgcta cgtcagctgg tgagaacgga ccatagcaat atccgtgtgc
    14941 taagcatgta tgcgtttaat gcctttgagc agcagcgatt tggcgaagcc gttgccgcgt
    15001 gggagatgat gttgaaactc ttacctgcca acgatactcg ccgtgcggtg attgaacgta
    15061 gtatcgcgca ggcgatgcaa catttgtcgc cgcaggagag taaataaaaa gaggcccgat
    15121 ggtgatgcca tcgggctatt tttattgtgc cccgcgttgt tgcaggaaca gaatggtggc
    15181 cgccacgcgt gagcggacat tgagtttacg cagcagattt cgaatatgaa cttttactgt
    15241 ctgctcggaa atattcaaca cagaggcaat ctgtttattt gacagcccct gtgccagctc
    15301 gtgcagaaca tccagctcgc gctccgtcag cacgctgaag ggatcttctt ccgcgccaaa
    15361 catttcacgt tcacgtaagt actgattgac gcgttcgcta aagactttgc tgcctttcgc
    15421 tccggcacga atcgcttcca gcaacacttc cgggtcgctg tctttcagca gataaccgtc
    15481 tgcgcctgcg tctatcagcg caaagacatc gctggaggca tcggatacgg tcaggataat
    15541 aatttgcgcg gtaacgccat ccctgcgcaa ggcattgagg gtatccaggc cactcatacc
    15601 tttcatattg agatccagca agatcacgtc gatatccagt ctattcgcca gatcgatcgc
    15661 gctcgcgccg tcgcccgctt cggcgaccac ttcaaagcca ggatcaagct ccagtaactg
    15721 acgaacaccg cgtcgcataa gtggatgatc atccacaatc atcacctgaa aaggcgttgc
    15781 ttcaggcata gtagtctcct gaggttttat tagaatcatt atgtttgagc gtagcgttgg
    15841 taacgctacc aggcgcgtga atgttacccc attttatgta tggacatgta gtgaaaacta
    15901 caaccttgcc gttcgctttg gcatcatttt cattataaag acggaatttt gcatcagctt
    15961 ctcatcaaga aaaacaacta aaaaatcaat cacaatactt gtgcatttct ttaaacagtt
    16021 tttaaaaact gactccgggt atggagctat gggtattttc tgtacccaat gcttttaaca
    16081 gcaattaatt tcataggatg aaagctcaat gcatcaatct ggttctgttt ctctttgtcg
    16141 ttccgcaata tctgttctgg tggctacagc gttatattca cccatagcat tggcatcaac
    16201 agttgagtat ggtgagacag ttgatggtgt tggcctggaa aaagatatcc agctggttta
    16261 tgggaccgcc aataatacga aaatcaatcc tggcggagaa cagcatataa aagaatttgg
    16321 tgtaagtagt aatactgaaa ttaacggcgg gtatcagtac attgaaatga atggcactgc
    16381 agaatactca gtattaaatg atggttatca aattgttcaa atgggtggcg cggtaaacca
    16441 gactacgctc aataatggag tgctacaggt ttatggcgct agactggccc cctgaatctc
    16501 cagacaacca gtatcactta t
//
LOCUS       DAZPAC010000081        16461 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZPAC010000081 DAZPAC010000000
VERSION     DAZPAC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.081"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(30..182)
                     /locus_tag="ACHUPM_002201"
     CDS             complement(30..182)
                     /locus_tag="ACHUPM_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0869973.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(447..1181)
                     /gene="cysH"
                     /locus_tag="ACHUPM_002202"
     CDS             complement(447..1181)
                     /gene="cysH"
                     /locus_tag="ACHUPM_002202"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR0869974.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1256..2968)
                     /gene="cysI"
                     /locus_tag="ACHUPM_002203"
     CDS             complement(1256..2968)
                     /gene="cysI"
                     /locus_tag="ACHUPM_002203"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR0869975.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2968..4767)
                     /gene="cysJ"
                     /locus_tag="ACHUPM_002204"
     CDS             complement(2968..4767)
                     /gene="cysJ"
                     /locus_tag="ACHUPM_002204"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR0869976.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5086..5448
                     /gene="queD"
                     /locus_tag="ACHUPM_002205"
     CDS             5086..5448
                     /gene="queD"
                     /locus_tag="ACHUPM_002205"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR0869977.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5526..6797
                     /locus_tag="ACHUPM_002206"
     CDS             5526..6797
                     /locus_tag="ACHUPM_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0869978.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6788..7048
                     /locus_tag="ACHUPM_002207"
     CDS             6788..7048
                     /locus_tag="ACHUPM_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR0869979.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7065..7640
                     /locus_tag="ACHUPM_002208"
     CDS             7065..7640
                     /locus_tag="ACHUPM_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFR0869980.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7788..8648)
                     /locus_tag="ACHUPM_002209"
     CDS             complement(7788..8648)
                     /locus_tag="ACHUPM_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR0869981.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8645..9424)
                     /locus_tag="ACHUPM_002210"
     CDS             complement(8645..9424)
                     /locus_tag="ACHUPM_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR0869982.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9402..10811)
                     /locus_tag="ACHUPM_002211"
     CDS             complement(9402..10811)
                     /locus_tag="ACHUPM_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0869983.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10833..12287)
                     /locus_tag="ACHUPM_002212"
     CDS             complement(10833..12287)
                     /locus_tag="ACHUPM_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR0869984.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12357..13173)
                     /locus_tag="ACHUPM_002213"
                     /pseudo
     CDS             complement(12357..13173)
                     /locus_tag="ACHUPM_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13491..14768
                     /locus_tag="ACHUPM_002214"
     CDS             13491..14768
                     /locus_tag="ACHUPM_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFR0869985.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            14795..16280
                     /locus_tag="ACHUPM_002215"
                     /pseudo
     CDS             14795..16280
                     /locus_tag="ACHUPM_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         3891 a         4344 c         4155 g         4071 t
ORIGIN      
        1 gtacgcgggg ttttaccccc gcgtggtggc tacttcttcg attcgtaagc gagaactgcc
       61 ttaaactcca taccacgttc tctgatactc aactcacaca acgagtcgcc taccatcagc
      121 gtgaatccca gtactgtaaa acacagcact atgattgcca caagggcata ttttgtcagc
      181 atgtcttgcc tccctgcaaa gaagagacta acattcgaat tgtttgggtt cgaacgctgg
      241 cctcaggttg atagaaatat cgcctggggc ttttgtccat ctggaacctc gcgaatgctt
      301 aacgccagac agcctcaagc acccgacgcc attctatacc tgataattct tccagcggcg
      361 ttcacgttta aaatcaggaa tactgctcgc aaaataaaaa cgcccccatc atttctgaca
      421 gaggcgttta atttatccgg caatatttac ccttcgtgca atccacattc cctttttaag
      481 ccaaagaaac gtgtttcttc ttccgacatg ccaggttccc atttacgggt tgtatgggta
      541 tcaccgaccg ataaatatcc ttcatcccat aatgggtgat atttcaggcc atgtttttgc
      601 aggtactgat aaatagttcg gttatcccag tcgataatcg gcagcacttt aaatacgcca
      661 cgctgaattg ccagcaccgg taaattggcg cgactgccgg attgttcacg gcgcaggcca
      721 gcaaaccagg tttgcgcgtt aagttctttc agagcccggt tcatcggttc gactttgttg
      781 atgtcattgt acttttcaat gccttcaacg ccctgctccc acagttttcc gtagcgtgct
      841 tcctgccagg ctgcgctttc ggtagcacgg tacactttca ggttgagctt gagtttgtcc
      901 gttaactcgt caataaagcg gtaggtttcc gggaacaagt aacccgtatc ggtgaggatc
      961 accggaatat ccgggcgaat ttgattcacc agatgcaggc tcaccgccgc ctgaatgcca
     1021 aagctggaag aaagtacata ttcaccgggc agattatcca gcgcccaggc tacgcggcct
     1081 tcagcgtcca gtttttccag ttcggcgtta gtttccgcca gcgccagaat acgatctacc
     1141 ttcggcagtt cgttcagggc gtttagatcg agtttggaca tagattcctc actgtttgcc
     1201 ttgcctgatg cgacgctcac gcgttcttat caggcctaca agaccgggct gatggttaat
     1261 cccacaaatc gcgcgccgga tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt
     1321 cgccgaagcc ttcacccgct tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg
     1381 acgccaggat ttccggctcg gtgatgtttt ctttatacat ccgtgggata cgtgtcccaa
     1441 tgcggttgcc gccaagatgc aggttgtagc gacccggcgc tttacccacc aggcccactt
     1501 ccgccagcat cgcgcgacca caaccgttcg ggcagcctgt tacacgcatc acgatatgct
     1561 catcgctgac accatgtttc gccattaaat tatcgatgtt gtcgataaaa gacggcagga
     1621 aacgctctgc ttccgccatc gccagcgggc aagtcgggaa tgacacgcag gccatcgagt
     1681 tttcacgctg cggcgtgacg gcattcatta acccgctctc tttggcgatc ttctcgatct
     1741 tcgctttctc gctttccggt acaccggtga tgatcagatt ctggttcgcc gtaatgcgga
     1801 aatcgccttt gtggatcttc gcgatctcca gcaggccggt tttcagcgga cgccccggat
     1861 aatcaaggat gcgaccattt tcgataaaca gcgtcaggtg ccagttatca tcaatgccct
     1921 taacccagcc aatacgatcg cctcgtccgg tgaactcata tggacggatc ggttcaaatt
     1981 tgatccccgc gcgacgctcc acttccgctt taaacgtctc aacccccacg cgctccagcg
     2041 tgtatttggt tttggcattt ttacgatcgg ttcggttacc ccagtcacgc tgagttgtca
     2101 cgacggcttc ggccaccgcc agcgtatgct ccagcggcag atagccaaac tcgctcgccg
     2161 tgcgggcgta ggttttcttg ttgccgtgtt cgatagaaag cccaccgccc accagcaggt
     2221 tgaagcctac cagcttgccg ttttcggcga ttgccacgaa gttcatgtcg ttggcatgca
     2281 gatcgatatc gttctgcggc gggatcacta ccgtggtttt gaacttacgc ggcaggtagg
     2341 tctgaccaag gatcggttct tcatcagtag tggcgacttt ttcctggtcg agccagatct
     2401 ccgcatacgc gcgggtacga ggcaacagat gctcagaaat cttcttcgcc cactcgtacg
     2461 cttccgcgtg cagctgcgac tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt
     2521 cgttagctgt cgccagcgca tcaagaccga ccgagtgcag catctggtgc accggtttga
     2581 cgttcttttt cagaatgccg tggaactgaa acgtctggcg gttggtcagg cgaatgctgc
     2641 catagatggt gttttcaccg gcaaatttgt cgatcgcctg ccactgttta gtggtaatca
     2701 ccccacccgg cagacgacag cgaagcagca tcgcgtggcg cggctccagc ttctgttcag
     2761 cacgttcggc acggatgtcg cggtcatcct gctgatacat gccgtggaag cggatcagca
     2821 ggaagttgtc gcccttaaag ccgccggtca gaccgtcgtt taaatcttcc gcaatggtgc
     2881 cgcgcaggta gttgctttca agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca
     2941 ctaaaggccc tggatgtttt tcgctcatta gtagacatct cgctgataac ggcgctctac
     3001 gcgcagctca cttaaaaatt catccgccgc ttcggtgtcc atgccaccaa attcggcaat
     3061 cacttccaga agtgcctgct caacgtcttt cgccatgcga ttagcgtcgc cgcagacata
     3121 aatgtgggca ccgtcattga tccagcgcca cagctccgcg ccctgttcgc gcagtttgtc
     3181 ttgtacataa actttttctt tttggtcgcg cgaccaggca agatcgatac gcgtcagcac
     3241 gccctctttg acgtagcgct gccactccac ctggtagagg aaatcttccg taaagtgcgg
     3301 gttgccaaag aacagccagt ttttacccgg cgcttcgtcg gcggcgcgtt gctgcataaa
     3361 ggcacggaac ggcgcgatgc cggtaccggg gccaatcata atcactggcg tttgcgggtt
     3421 agcgggcagg cggaaattgt cgttatgttc gataaatacg cggacttcgc cctcttcttc
     3481 cacgcgatcc gcgaggaagc tggaggcacc accggcacgg gcgcggcctt ccacgtcgta
     3541 acgcaccaca ccaacggtga cgtgtacttc gttctcgact tccgcctgcg aggaggcgat
     3601 ggaatacagg cgcggggtca gcgggcgcag caggttaatc agcgcttcgg catccagttg
     3661 cgccggagag aaacgcacca tgtcgacaat cggcgtggtc gcggcgtaat gctgtaactt
     3721 cgctttatcg cccaccagcg gcagcagcgt ttcgctgcgg gtaagcgtgg catagttctc
     3781 aacaatgttg gcggtgttga cggtcagttc gaagtgccac tgtagcgctt cgttcagagg
     3841 caacgttttg ccctcgacgg tgacaggttc atcgcctttc agccacagca gttcgacaag
     3901 ttctttcacc agtgccgggt cgttctgata ccagacaccc agcgcgtcac ccggctggta
     3961 acgcaggcct gaatcgccta agtcaatttc gatatgacga acgtcttttt cagagttacg
     4021 cccggtaatt ttctggttaa ccgaaaggct cgccaccagc ggcgcgtctt tgctgtacgg
     4081 gctggtgtgg atttcattta ccgcgccagt agcgacggat tgcgaaggtg ccgcgacagg
     4141 cgcacgcgat ttaagcgcat caaccacgcg ggcgcgccac tcgctggcag cagcctggta
     4201 ttcaacatcg gcatcgacac ggtcgagcag gcgttcacca cccagttccg ccagcttgct
     4261 gtcgaaatct ttcccggact ggcagaaaaa ttcataagag ctatcgccga ggctaaacac
     4321 ggcaaacgcg gtgttttcca gctttggcgc ttttttggag aacaggaact tatgcagcgc
     4381 gacggcttct tccggcggtt ccccttcccc ttgcgttgac gtcactacga tgagcagttt
     4441 ttcgctggcg atttgtttga atttatagtc gcccgcgttc accagcttaa cgttcagttt
     4501 tgctgctaat aaatcatcgc gtaatgcttc agcaacccgg cgcgcattgc cggtttgcga
     4561 ggcggagata atagttatac ccggcatttc tgcggctggc gctggcgtcg ctgcaagcgc
     4621 agcaggctgc tgattgagta cgccccagaa atagccagaa acccaggcaa gctgggtagg
     4681 cgttaaatcg gtcgtggccg cctgaaggcg tgccagttgc tccgggttca acggaagcaa
     4741 cgcggaaggt gggacctgtg tcgtcatgcg tcgttatgtt ccagtaagca aagctgtttc
     4801 tgcgccctgt cagcgcccat aaaacagaag agaagataag gttaacgggg tgaacggtgt
     4861 ggattaaaga cgggatagcg ataactaata accaaatcga ctaacctgtt ttagcaatag
     4921 tctttaacaa caaaatagat taaccaaccc aatgaaaaac aaatgaattt agccaatcat
     4981 taagataaat cagcgatttt gcgcaacaaa gtcgttttag ataatgcgaa aaaacagcct
     5041 ttccggtact ctacggcggt tttatcgtcc tgtagagaaa ttatgatgtc caccacgtta
     5101 tttaaagatt tcaccttcga agccgctcac cgcttaccac acgtcccgga agggcataaa
     5161 tgtggtcgcc tgcacgggca ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat
     5221 ccgcatacgg gctggattat cgatttcgct gaactgaaag cggcgtttaa gccaacttat
     5281 gagcgcctcg atcaccatta tctcaatgat attccaggtc tggaaaaccc aaccagcgaa
     5341 gttttagcaa aatggatttg ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg
     5401 atggtaaaag aaacctgcac cgcagggtgt atctatcgcg gcgaatgata agagtgtgtc
     5461 ggcggtcaat ttcccttaag taacgctatg ttagggtgtt gtgttctgga tatctggggc
     5521 atgacatgga agacgactgc gacattatta ttattggtgc cggtattgca ggcaccgctt
     5581 gcgcgttacg ctgcgcgcga gcgggtttat ccgttttgtt actggaacgc gctgaaatcc
     5641 ccggcagcaa aaatctttcc ggcgggcggt tatataccca tgcactcgcg gaactcctcc
     5701 cgcaatttca tctgaccgcg cctcttgaac gacgcatcac tcacgaaagc ctttccctgt
     5761 taacgcccga tggcgtaacg acgttttcca gcttacagcc cggcggtgaa tcctggagtg
     5821 tattacgtgc acggttcgat ccgtggctgg ttgccgaagc cgaaaaagaa ggtgtcgaat
     5881 gcatccccgg agcgacggtg gatgcactgt atgaagaaaa cggcagagtc tgtggcgtta
     5941 tttgtggtga cgatattctc cgcgcccgtt atgtggtgct ggcagaaggt gccaacagcg
     6001 tcctggctga acgtcacggg ttagtgactc gtcctgctgg cgaagcgatg gcgttgggga
     6061 tcaaagaagt gctgtcgctg gaaacatccg ctattgaaga acgttttcat ctggagaata
     6121 acgaaggcgc agcgttgctg ttcagcggcg gaatctgtga tgacttaccc ggcggcgcat
     6181 ttctttatac taatcaacaa acgctctcgt tagggattgt ttgcccgctc tcttccctta
     6241 cgcaaagtcg tgttccggca agcgagctgc tgactcgctt taaagcgcat ccggcagtgc
     6301 gcccgcttat caaaaacacg gaatcactgg agtatggtgc gcatctggag ccagaaggtg
     6361 gcttgcacag tatgccggtg caatacgccg gtaacggctg gctgctggtg ggcgatgcgt
     6421 tgcgcagttg cgtcaatacc ggaatttccg tgcgcggcat ggatatggcg ctgactggcg
     6481 cacaggcggc ggcacaaacg ctaataagcg cctgccagca ccgcgagccg caaaatctgt
     6541 ttgcgcttta tcatcacaac gtagagcgca gcctgctatg ggatgttcta caacgttatc
     6601 agcatgttcc ggcgcttttg caacgccctg gctggtatcg ggcgtggcct gcgttaatgc
     6661 aggatatttc ccgcgattta tgggatcagg gtgataaacc tgttccaccg ctgcgccagt
     6721 tattctggcg tcatttacgt cgtcatggcc tgtggcatct ggcgggcgat gttatcagga
     6781 gtctgcgatg tctgtagccc ataatctctg gcgcgttgct gatgcgccgc acattgttcc
     6841 ggctgactcc gttgagcgtc agatggtaca acggttgatt aacgcctgtc cggcaggtct
     6901 tttttcgctc acaccggaag gtgacttacg cgttgattat cacggctgcc tggagtgtgg
     6961 cacctgccgt ttgctgtgcg acgaatccac actacaacag tggcgctatc cgccttccgg
     7021 attcggcatc acctaccgct ttggataaat aataaggata atttatgccc ctcttacacc
     7081 tactccgcca gaatccggtg attgctgccg ttaaagataa tgccagcctg caactggcta
     7141 tcgattctga atgccagttt atttccgtgt tgtacggcaa tatctgcacc atcagtaata
     7201 tcgttaaaaa gattaagaac gccgggaaat atgcttttat tcatgttgat ttgctggaag
     7261 gcgcgtcaaa taaagaagtc gttattcagt ttttgaaact ggtgaccgag gcggacggca
     7321 ttatcagtac caaagcctca atgttgaaag cggcaagagc ggaaggtttt ttctgtattc
     7381 atcgcctgtt tattgttgat tcaatttcgt ttcacaacat tgataagcaa gttgcgcaat
     7441 cgaatccgga ttgtattgag atcctgccag gcggtatgcc caaagtgctg ggctgggtga
     7501 cagagaaaat ccgccaaccg ctgattgccg gtgggctggt gtgcgatgaa gaagatgcgc
     7561 gtaatgcgat taacgcgggt gtcgtggcgc tttccaccac gaataccggg gtctggacgt
     7621 tagcgaaaaa attactttga cgggataatc gtaaccaatt gaatttggtt tgatttgtag
     7681 gccgcacgcc acatccgaca ttcagcgcct gatgcgacgc ttgacgcgtc ttatcaggcc
     7741 tacaagtccc caggccagaa ccgtaggcag gatcatccgg caacacgtta ttggcaatct
     7801 gcgtgaatgt ttgtcaccag tgcctccagc accactttcc agtcatcaac cacgccaaca
     7861 tctgcctgtg aaaataccgc agcgctggcg tcatgattaa tcgccaccac aaacttgcta
     7921 ttgcgtacgc ccgccattaa cgctgctgcg ccagaagcgc caacaacaat acacacctca
     7981 ggtgccagca gatgtccgga aataccaatc actttttcag catcaacgcc accgttcatc
     8041 actcgcgccc gactgtagcc cacttcggct cccagctttt ctgccagcat agcgatttcc
     8101 tggttatctg cctctccgcc ctgccccacc accagcaccc gtctggcctc agctaatggg
     8161 tcgcgagtaa catttttcag atcctctgtg ctaaccagcc agtccggaag cgcacccggg
     8221 acaatgttca gttgttgcat tccagagggc aacgtggcgt ttttcgctgc tcccgcctga
     8281 cgcgccaggg aaaggcatag cgggcgtttt tctgtttgca gcgtcgccgt tagcgcattt
     8341 ccccagtggg atttgcgcac gcttacggtt ggaatatcta acgaagtcac ctggcagatg
     8401 cttgcaccat gtaaacgcca ggccagccgg gtcgacagtt catcgccaaa cgttcccggc
     8461 ggaaagagaa caacgtccgc aggcgttcgc tgccactgtt ccaccaacgc atccagcacc
     8521 tgttccgcca ctacaggctg aggttcaatc tgccagtgcg ccagggtaca accgctgaaa
     8581 tcctgcgccg ccagccagct tgctattgcg gcattctcct gatttattgt cacaattgcg
     8641 atattcatgg ctgcatcctt tggcgtaaat agtcctgcca tagcttttga gctttttctg
     8701 ctaccgtttg cccatcaatc agcgtcgctc cgcgtcgttg ctctgctctt gccagttgca
     8761 gacactgcat cgctggcatt tcggcagcaa cagttttacg gataatttct gctttccccg
     8821 cagccattcg ctggcgcatt cccggtactg gcaaggccac ttcaccgcac tgacgcacgg
     8881 caatcaccgc aggcaagcga acccggcaac aacgcaaccc atgttcagta cgctgttcga
     8941 gggtgataaa cagcgcgtcg agagtgaaac gctccacctg ggtgaagcag ggccagccca
     9001 gcatttccgc cagcaaaaat ggcgtttgcc cattctgccc ttcgctgctt tggcagccgg
     9061 taattatcag atccagcgga ttctgatgct gccattcggc aatatggcga gcgacaaatt
     9121 ccggcgcaaa gcgcagatct gccgccgtct ccagcaatac agcttcctca aacccaagag
     9181 ccatgagata gcgcagccag tgcaatgccc gttcatcccc catgctcaac gcggttaaag
     9241 acatcggcgt accgtttttt ctctgcgcca acagcagcgc ggcagcggcc tgttcatcag
     9301 caccgagtaa acttcgcagt agcgaaatat ccggtccgct tttaccctga gccgccgcct
     9361 gccactcttt ttccgccagc attccggcat ccggttcggc tttaaacgct aacagaatgt
     9421 tcattcgcac ctcctacatt tcccgccgcc accagcggga gggctttagt ttctggtgcc
     9481 catagccagg tcacaacaaa atcaaccaac aacactgtcg ccaggagcaa taaggtgact
     9541 tgcattcccc actgcgccag cacccacggc agcaggccag tacttaccgc cgcgccaagt
     9601 cgactcatgg cggtggcaaa accgacgccc agcgagcgaa tgtcagtagg aaaactttcc
     9661 gcaggcaaaa tgcccaccag attactgact gccgaaatgg tggtgctgaa gagaacaaaa
     9721 agcagcagcg ttaatgaact gccggaaggc aaacaggcca tcactaccag cgttgccgcc
     9781 agcagcaaaa aacttcccag caaaaatttg cgatgtgcca gcaggtgcgt cagaactaat
     9841 cccagcagcg cgcccacaat taacaacgca ttaagcatca ggctggcagt cagcgcatct
     9901 tccagaccaa tagtctgggc gatagttggc agccaggtat aaatcacaaa ccatgggatt
     9961 acgaggcaga caaagaagac gctgttaaac gccgtgcgcc gccagtaacg cgaagagaac
    10021 aaggttttga tgtgtttatg ggtcgccgtt accacttcat cgcccagtaa aacatgggga
    10081 ccaaaatagc gatgcacgat agcgtgagct tctgcaaaac gcccctggcg cagtagccag
    10141 cgtggtgatt ctggcgttcc ccagcgtaat aacgtaatca acaacgcggg cagagctgcc
    10201 gatgccagta gccagcgcca ggcctccggg ttttcggaaa taaagtgatg tccggcaata
    10261 cttgccagca catagcctac ggtccacacc acgctgaatg cgcccagcaa aataccgcga
    10321 tggcggcgcg gggaaaattc agccagcaag gtgtgaccta ctgaataatc gcctcccaga
    10381 ccaatgccaa tcaaaatgcg cagtccaata agatgctctg gcgtggtggc aaaaaattgt
    10441 aaaaacgaag caagcgtaat cagcaaaaag ctgaaggtga agattttttg ccgaccaata
    10501 tggtcggaga tccacccaag aaccaggctg ccaaggaaca aaccaaggag cgccgagccg
    10561 ccgatcattc ccgccataaa cggcgtcagt tgcatggcgg gcgtaagctg aataatggcg
    10621 taaccaatga cgccgagaac ataaccgtcg gtcaggtgtg cgccgaaagt gagcgcagca
    10681 atgcggcagt gaaaacggtt aagcggtaaa tcatccattc gcaccggtga agtgttcatc
    10741 tttacccctt cattgtccgg taataagaac ggcttctcgc ctgagaagcc gaaaaggcaa
    10801 cgccccgtca tcgcggaacg ctgcctgaca cattattttt caatcggata gatagtaccc
    10861 gtattcataa tgccattagg atcgaactgc tttttcagcc cttccagcaa cgcccacgcg
    10921 ctgccgtgtt ccagtttgct ccagtgaacg cgatgtttac cgataccgtg gtggtgcacc
    10981 atcgaaccgc cgaggcgaat ggtttcttca cagatgatct tgttgagcgg attgtggtac
    11041 ttgtcgattt cctcttccgg cttacagtca acgacgttgt aatcgtagac gaagtacatg
    11101 ttggtgccgt tctgatagct atgagaggaa tgaccgccca gcatggtgat gtcgtcggcg
    11161 tgcgggaact cagtacgaat acggttaata acgctttcgt agatttcgtg gatgcagctc
    11221 cagcagccgg acacttcagt ggtaaagccc atgttgccgg ttttgaggat ctgcacacgt
    11281 tcggcagcca ctttatccgg tccccagttc aggttgttaa accaggtttc gatcagcttg
    11341 ctgtccacgc gctggcattg cgggtagcgg gcaacgattt ccgcaatccc ttcgcccgtc
    11401 gccttcgcaa tgcgagggtt accttcagcc ataaagatca gcacgcattt tccgtctgca
    11461 aaatgggtga agtgttgggt gccatcttca gcgtcataca aacgagcgat cgacggacga
    11521 tacccttcca ccatgacttc acgcaggatg ttgaagtcgg ttttcatgtc ttccaggata
    11581 tagccgtaga agaggttgtt ttccggggtg aatttaaaga ttttcactgt tacttcagtg
    11641 atatagcaaa gtgcaccttc gttaccgatg atgatgtggc gaatgtccgg gccagccgcg
    11701 cggcgtggca cgtttttaat gcgtgtgacg gtgccatcag ccaatactgc ttccagacca
    11761 acgaccatat cttcgattgc gccgtagagt gtggagaact gcccgatact gcgggttgct
    11821 accaagccgc ccatctgcgc cagcggcttt gactgcggag aatgccccgt ggtgtaacct
    11881 ttttcacgca acgcattttc cagcacctcc agcggaaccc cacattgcgc cgtcgcctgc
    11941 atattctcaa tatcaatatt aatgatttga ttcatggcgg agccgtcaag caccaccgag
    12001 ttttccacaa cggtttccag cccaccttca gtggcggaag cgccggtacg cggcacaccg
    12061 ttaattttgt gcgcattcat aaaattcagc acacgggata cttgctctgt ggaaccgagt
    12121 tttacgaccg ctgccggaat cggcaaagta taaatgccat gaatatccgg aaatttacga
    12181 aaacggtcaa tactgttctt ctttaatact gtttcatcgg taattacgcg atctgcacca
    12241 acaatttcct ttagttggtc gacaatcgct gcgcgagata aagacatagt aattccttcc
    12301 tgataataaa aatggaataa tcattaataa gagaaaacag aaaccaccgt tatggattag
    12361 cgcactaaat aaccaccatt gaccactaat aaatgcccat tgacataatt tgaagccgga
    12421 cttgcgagga atacagctgc gcccattaaa tcctgagtat cgccccaacg gtttgccgga
    12481 atatgatcaa gaacgcgctg attggtttct ggattactgc gtgtcgccag cgtaatatct
    12541 gttgcataat agccaggagc aataccattt acctgaatat tatattgacc tagttcatca
    12601 caataagctt tggtgaatcc ggcaagagca tgtttagtgg cagaatatgc aggtgaccat
    12661 tgtccaccta agtaagagaa caatgaacag atattaataa ttttgccgct tttctgcggg
    12721 atcataattt ttgcagcttc atagcttaac tcgaatgcgg cggtcaggtt cacatcaatc
    12781 atcggatccc agtcggcacg accgaagtcc agcaccttat tcagcttaca aataccggca
    12841 ttgtttacca gaatatcaac tgtaccgaaa cgctcacagc aagcagcgat aatcttctgc
    12901 ggcgcgcctt ctgcggtgat atccacctgc atgaagtcca cctcaacacc ctgtttttca
    12961 atcatttcct ttgtttcgcc gttatctttc aatcatttcc tttgtttcgc cgttatcttt
    13021 gacgaaacta ggaataaaga tatttgcgcc agctttggcc aacgccatgg caaatgcctg
    13081 gcctaaaccg ctattcccac cggtaacaat tgcggtttta cctttcagtg agaaaaaatc
    13141 cattgagaac gcattgagag attcgattga catagttagc tccaatttcc gtaaggcaaa
    13201 aaaaagaaag gtaacttccc ccgcgagagg gaagttacct ttctcattat ctctggtaac
    13261 tggagataat ttttagcata acggcataac tgctaatgtg atcgtaatca cagtgtgata
    13321 ttcgtattgt caaatagttt taaaaagcag aagcgtgatt actctcacac aacacctctg
    13381 ctttccatgt tatttcttat tgcaattact agagacctga gaaaagtaat ccacctgaaa
    13441 gggcggctta cttttctctt ttttttgtct attacaaatg ggaatacgat atgcaacaca
    13501 actcatatcg ccgttggata accctcgcga taattagttt tagcggcggc gttagttttg
    13561 acctggctta tttacgttat atttttcaaa ttcccatggc gaaatttatg ggattcagca
    13621 ataccgagat aggtttaata atgagtacct ttggtattgc ggctattatt ctttatgccc
    13681 ccagcggcgt tattgccgat aaattttcac accgcaaaat gattacttcc gcgatgatca
    13741 ttaccggatt actgggtctg ttaatggcaa cgtatccacc gctgtgggta atgctctgta
    13801 ttcaggtcgc ctttgcgata acgacgattt taatgctgtg gtcggtgtcg attaaagccg
    13861 cctcgttgct gggcgatcat agtgagcaag ggaaaattat gggctggatg gaagggctgc
    13921 gcggcgtcgg tgtaatgtcg ctggcggtgt ttaccatgtg ggtcttttct cgctttgcac
    13981 cggatgacag cgccagcctg aaaacagtca ttatcatcta cagtgtggtt tacatcttgt
    14041 tggggattct gtgctggttt tttgttagcg ataacaacaa cctgcgcagt gccaataacg
    14101 aagaaaaaca gtcattccag cttagcgaca tccttgccgt tttgcgtatc agcaccacct
    14161 ggtattgcag catggtgatt tttggtgtct tcacaatcta cgccattctg agttactcca
    14221 ccaactatct gaccgaaatg tacggcatgt cgctggtggc agcgagctac atggggattg
    14281 tgatcaacaa aatcttccgc gcgctgtgcg gcccacttgg aggcattatc accacctaca
    14341 gtaaagtgaa atcccctacc cgcgtgattc aaatcctttc catcatcggt ttgctggcgt
    14401 tgacagccct gctcgtcacg aactctaacc cccaatcggt cgcgatgggg attggcctga
    14461 ttttactgct gggattcacc tgttacgcct cacgcgggct gtactgggcc tgccccggcg
    14521 aagcgagaac accgtcttac attatgggca ccacggtagg tatttgttcg gtgattggat
    14581 tcctgccgga tgtcttcgtt tacccgatta tcggccactg gcaagacacc ctgcccgctg
    14641 cagaagccta ccgcaatatg tggctgatgg gcatggcggc gctcggcatg gtgattgtct
    14701 ttaccttttt gctgttccaa aaaattcgta ctgctgatag cgcccccgca atagctagca
    14761 gcaagtaagc caggaaaatt acaggagaaa aatcatgtcg aaaaaataca tcatagggat
    14821 tgatggcgga agtcagagca caaaagtggt gatgtacgat ctggaaggta atgtggtttg
    14881 cgaaggtaaa ggcttattac agccgatgca cacgcctgat gccgatactg cagaacatcc
    14941 tgacgacgat ttatgggcat cattatgttt tgcaggtcac gatttgatga gccagtttgc
    15001 cgggaataaa gaagatattg ttggcatcgg cctgggctcc atccgttgct gccgtgcgtt
    15061 attgaaagcc gacggcacgc cagctgcgcc gttgattagc tggcaggatg cacgcgttac
    15121 acgcccttac gaacatacga atcctgacgt ggcgtatgta acctcttttt cgggttatct
    15181 gacgcatcgc ttaaccggcg aatttaaaga taatatcgcc aactattttg gtcagtggcc
    15241 ggtggattat aagacctggg catggagcga agatgccgca gtcatggaga agtttaatat
    15301 cccccgtcat atgctgtttg atgtgcaaat gccaggcact gttcttgggc atatcacacc
    15361 tcaagccgcg ctggcgacgc atttcccggc aggactgccg gttgtttgta ccaccagtga
    15421 taaaccggta gaagctctgg gagccggatt actggatgat gaaactgcgg taatttctct
    15481 tggcacttac atcgcattga tgatgaacgg caaagccttg ccgaaagatc cggtggcgta
    15541 ctggccgatt atgtcttcta ttccgcaaac attgctgtat gaaggttacg gtattcgcaa
    15601 aggcatgtgg acggtgagct ggctgcgcga catgttaggc gagtcgttaa ttcaggatgc
    15661 cagggcgcag gatctttcac cggaagattt actcaacaag aaagcctcta gtgtgccacc
    15721 tggctgtaat gggctgatga cggtgctgga ctggctgacc aatccgtggg aaccgtacaa
    15781 acgcgggatt atgatcggct ttgattccag catggattac gcatggatat atcgttcgat
    15841 attggaaagc gtggcgctga cgctgaagaa caattacgac aatatgtgta atgaaatgaa
    15901 tcactttgcg aaacatgtaa tcattactgg cggcggttcg aacaggatct gtttatgcag
    15961 atttttgccg acgtgttcaa ccttccggca cgccgtaacg ccattaacgg ttgtgcaagc
    16021 ctgggggcag cgattaatac aggggtaggt ctggggctat acccggatta cgcaacggct
    16081 gtcgataaaa tggttcgcgt gaaagatatc tttataccga ttgagagcaa tgccaaacgc
    16141 tacgacgcga tgaataaagg cattttcaaa gacctaacca aacatactga tgtgatcctg
    16201 aaaaaatcgt atgaagtgat gcatggggaa ttggggaatt ggggaatgcg gattcgatcc
    16261 agagctggtc gaatgcgtga ataaggtagg ccgggcggaa aactgcccgg cctgaacata
    16321 cctgaatggt tatccccgct ggcgcgggga acataagttc gacaacttaa aaaatgataa
    16381 caagtccggt ttatccccgc tgatgcaggg aacataataa aaactttaac tagttcggtt
    16441 tatccctgtt tttacaagga a
//
LOCUS       DAZPAC010000082        16359 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZPAC010000082 DAZPAC010000000
VERSION     DAZPAC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.082"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            447..1664
                     /gene="alaA"
                     /locus_tag="ACHUPM_002216"
     CDS             447..1664
                     /gene="alaA"
                     /locus_tag="ACHUPM_002216"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR0869986.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1748..2347
                     /gene="yfbR"
                     /locus_tag="ACHUPM_002217"
     CDS             1748..2347
                     /gene="yfbR"
                     /locus_tag="ACHUPM_002217"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR0869987.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2406..4238)
                     /locus_tag="ACHUPM_002218"
     CDS             complement(2406..4238)
                     /locus_tag="ACHUPM_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR0869988.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4325..4973)
                     /gene="hxpA"
                     /locus_tag="ACHUPM_002219"
                     /pseudo
     CDS             complement(4325..4973)
                     /gene="hxpA"
                     /locus_tag="ACHUPM_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(4984..5478)
                     /locus_tag="ACHUPM_002220"
     CDS             complement(4984..5478)
                     /locus_tag="ACHUPM_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR0869989.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5561..6016)
                     /gene="yfbV"
                     /locus_tag="ACHUPM_002221"
     CDS             complement(5561..6016)
                     /gene="yfbV"
                     /locus_tag="ACHUPM_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR0869990.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6354..7556
                     /gene="ackA"
                     /locus_tag="ACHUPM_002222"
     CDS             6354..7556
                     /gene="ackA"
                     /locus_tag="ACHUPM_002222"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR0869991.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7631..9775
                     /gene="pta"
                     /locus_tag="ACHUPM_002223"
     CDS             7631..9775
                     /gene="pta"
                     /locus_tag="ACHUPM_002223"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR0869992.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9965..11485
                     /gene="yfcC"
                     /locus_tag="ACHUPM_002224"
     CDS             9965..11485
                     /gene="yfcC"
                     /locus_tag="ACHUPM_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR0869993.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11518..12060)
                     /gene="yfcD"
                     /locus_tag="ACHUPM_002225"
     CDS             complement(11518..12060)
                     /gene="yfcD"
                     /locus_tag="ACHUPM_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR0869994.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12118..12669)
                     /gene="yfcE"
                     /locus_tag="ACHUPM_002226"
     CDS             complement(12118..12669)
                     /gene="yfcE"
                     /locus_tag="ACHUPM_002226"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR0869995.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12725..13369)
                     /gene="yfcF"
                     /locus_tag="ACHUPM_002227"
     CDS             complement(12725..13369)
                     /gene="yfcF"
                     /locus_tag="ACHUPM_002227"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR0869996.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13505..14152
                     /gene="yfcG"
                     /locus_tag="ACHUPM_002228"
     CDS             13505..14152
                     /gene="yfcG"
                     /locus_tag="ACHUPM_002228"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR0869997.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14209..14571
                     /gene="folX"
                     /locus_tag="ACHUPM_002229"
     CDS             14209..14571
                     /gene="folX"
                     /locus_tag="ACHUPM_002229"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR0869998.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14592..15485
                     /locus_tag="ACHUPM_002230"
     CDS             14592..15485
                     /locus_tag="ACHUPM_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR0869999.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15533..>16359)
                     /gene="rpnB"
                     /locus_tag="ACHUPM_002231"
     CDS             complement(15533..>16359)
                     /gene="rpnB"
                     /locus_tag="ACHUPM_002231"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR0870000.1"
                     /translation="TARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLLW
                     SVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFYH
                     GCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQKH
                     IRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQEK
                     EKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         4094 a         4347 c         3983 g         3935 t
ORIGIN      
        1 agcacatcaa aacctttttg aatattaaat aataattaat tagcatcatc ctcattcatt
       61 aattccgctt aacaatagtt tcacaccttg cccccattgc gacacctcgg aaatcatcac
      121 gctgttaaac tctccgtaat gcggaaatat catcatcgcg tttcattagt gaattcttaa
      181 atgaggcatt ttcacacaat tatcttacag ataaaaaaac cagacttgca attaagaatc
      241 agaacaattc actatataac attgcatgca aagcatatac gcctcattat tttgtcatta
      301 ttaagttatt aacagcacaa tcgagccttc ccctctggca aaatcttatt ctgcagacct
      361 tcaaaacacc gtcctggggg agtacattgt tctaagctga cttccacggc agggagtggc
      421 gataacagca aaaaaggtca agattcatgt cccccattga aaaatccagc aaattagaga
      481 atgtctgtta tgacatccgt ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag
      541 gtaacaaggt actgaaactg aacatcggca acccagcccc attcggtttt gacgcgccag
      601 atgaaatcct cgttgacgtg atacgcaacc tgcctaccgc tcaagggtat tgcgattcca
      661 aagggcttta ctccgcgcgt aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg
      721 atgttaccgt ggaagatatt tacatcggca atggtgtatc ggagcttatc gttcaggcaa
      781 tgcaggcatt gctgaacagc ggggacgaaa tgttggttcc tgcaccagat tacccactct
      841 ggaccgcggc ggtttcgctt tccagcggta aagcggtgca ttatctttgc gatgaatcct
      901 ctgactggtt cccggacctc gatgatattc gcgctaaaat tacgcctcgt acgcgtggga
      961 tcgttattat caacccaaat aacccaaccg gcgcggtata ttccaaagag cttttaatgg
     1021 agattgtgga gattgcacgt cagcataatc tcattatctt cgccgatgaa atttatgaca
     1081 aaattctcta cgacgatgct gagcatcact caattgcgcc actggcacct gacctgctga
     1141 ccattacctt taacggactg tcgaaaacgt accgcgttgc aggcttccgt caggggtgga
     1201 tggtgttgaa cgggccgaaa aaacacgcca aaggctacat cgaaggtctg gaaatgctgg
     1261 cttcaatgcg cctgtgtgct aacgttcctg cgcaacacgc cattcagacc gcgctgggtg
     1321 gttatcagag catcagtgaa tttattaccc ctggcggtcg tctttatgag cagcgtaacc
     1381 gcgcgtggga actgatcaac gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc
     1441 tgtatatgtt cccgaaaatc gacgccaaac gctttaacat tcacgacgat cagaaaatgg
     1501 tgctggattt cctgttgcag gaaaaagttc tgttggtgca agggacggca ttcaactggc
     1561 cgtggccgga tcacttccgc attgtcacgc taccgcgtgt cgatgatatc gagctgtctt
     1621 tgagcaagtt cgcgcgtttc ctttctggtt atcatcagct gtaatcttaa tttcactgcc
     1681 ggagattgca tccggcagcg ttatcccgcc acaatgacct gatgatgtca tcatacgtaa
     1741 ggtcactatg aaacagagcc atttttttgc ccatctctcc cgcctgaaac tcattaaccg
     1801 ctggccgcta atgcgcaacg tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc
     1861 gatggtcgcc catgcgctgg cagctatcaa aaatcgaaaa tttggcggta atgtcaacgc
     1921 cgaacgtatc gctttactgg cgatgtacca cgatgcctca gaagtgctca ccggcgatct
     1981 ccctactccg gtgaaatact tcaattcgca aatcgctcag gagtacaagg ctattgaaaa
     2041 aatcgctcag caaaaactgg tcaatatggt tccggaagag ctgcgggata tctttgcgcc
     2101 gttaattgac gagcatgcat atagcgatga agaaaaatcg ctggtgaaac aggcagatgc
     2161 actgtgtgca tatctgaaat gtctggaaga actcgcggcc ggaaataatg aattcttgct
     2221 ggcaaaaacg cgactggaag cgacgcttga agcgcgtcgc agccaggaga tggactactt
     2281 catggaagta tttgttccca gcttccatct ttcgctcgat gagattagcc aggattcacc
     2341 gctgtaagca gccggagtct gcgtcgcatc aggcaataag cgccggatgc gacatcaggc
     2401 tcttgtcaaa acggaaacag catcgggatc atcaccacac aaaccgccat cacgataatg
     2461 gtgaacggta cccccaactt cacaaagtca ctgaagctgt aatttcccgg acctaaaacc
     2521 agtgtgttaa caggtgaaga aaccggtgtc ataaaggcgg cggatgctgc catcgccacg
     2581 accatcgcga atggataagg cgacaccccc atcgttttgg cagcagccag cgcaatcgga
     2641 gccatcaaca ccgccgtcgc ggtattagag ataaatagcc caataaccgc cgacaagaca
     2701 aacaaacacc ccagcatcat atgtggcccg taaccaccgc caatgtccat cagccctttc
     2761 accgccagcg cgacacctcc cgttttctgt aatgccacag caaacggcat catcccaacg
     2821 atcaaaataa tgctcggcca gtgaatggat ttataggcgc tttcagcatc tatacagcgg
     2881 aatttcccca tcagcaggca ggcgatgata gcggcaacag gattaggaat ttcatctgtc
     2941 agcattaacg ccaccattag caccagacag aaaatggcat ggggtgcctg gctgtgcgcg
     3001 ggcgatgctt cactcacctc ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg
     3061 gccagcatac cgatcagttt ccagttaccc acaaccagga tgatatcgcc cagcagcaga
     3121 ggctcatccg ccagcgaacc ttccagcgcc acgccattgc gctttagccc caccacattc
     3181 agtccgtagc gggtacgaaa accaatttcg cgcaccgatt taccaatcag ttctgactca
     3241 ggaattaatg aaatctctgc catgcccaca tcaagggcct ggtcagaaaa atactcgccg
     3301 cgcagtacca tcggctccag caattgctca ctacaaaatt gccggagatc gacatcagcc
     3361 gcagacatat caataagcaa aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc
     3421 acgataacgc gacgaaaacg ccgccagcgt tcaacaccga tgacgttagc gccataacgc
     3481 tcacgtaatt tgagatcatc cagccgttga ccaatcattg gcgatccggg gcgaatagcc
     3541 agacgtcgcg cacgcccggt cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc
     3601 gtccagcctt cgcgctgcgg ggtctgggta tcccctttca gcatgaaacg catcactaac
     3661 atatacaaaa tacccagcac cagcacgacc aggccaatag gtgttacgct aaagaaactg
     3721 aagccgtgat agccttcacg cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc
     3781 accagcgtca tcatgccgct tatcagcccg gcaaaactca acggcatcat cagacgtgac
     3841 ggcgaagttt gcatacgcat ggcaacgctt aacaccacgg gaataaagat agcgacaacg
     3901 ccggttgaac tcataaacgc gccaagcccc gcgacggtca gcatcaacaa aaccaacatt
     3961 ttgatttcac tgttgcccgc aactttgacc agccatgttc ccattacggt ggcaacaccg
     4021 gtacggacca aaccatcgcc aataataaac aaggcggcaa tcaggacaac gttaggatca
     4081 gaaaagccgg aaaatacttc ggggactgtc agcgttccgc ttaatgcaaa cgcgacaata
     4141 acaaacaaag cgaccgcatc catacgcact ctgcccgtcg caaacaagac aatggcaacg
     4201 gccagtaatg aaagaaccca aatcaattca ccgttcacaa cttatccttg ttaattgagg
     4261 gagatgtctt gattctgcca taaaaaagcc ccgacgagac ggggctaaat catgatcagg
     4321 tgtttcactg aataataaca tcgccatttg gctgtttggt cacagtaatt tgctccagac
     4381 tgtggaggac caaatcgacc tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa
     4441 tgacatgaca acccgccgcc aggccagaaa gcacgccagc ggagcatctt ccaccaccac
     4501 acactcctgc ggcgcaagcc ccagcagctg cgcgcctaac agatacgcat caggttctgg
     4561 ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa gcccagctat
     4621 tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc acggaatacc
     4681 tgctttattc aaatgactga gtaaggcgat tgcccccgga agcgggtaat accttcggtt
     4741 tccgtggcct cgatgtgctc cagacgcgta aactcggcgg caatatcagc ctcggatttg
     4801 cccgccataa aatggcgcag agaggtgatc gcctgtttac cgtgaatgaa agccagcacc
     4861 tcttccggcg ctaacccatg acgtctggcc cagttgctcc acgcccgttc taccgcaggc
     4921 agggaatcca ccagcgttcc atcaagatca aacagaaaac ctttgcaccg cacgcgggcc
     4981 tcctcaggca ttgataattt gattaatttc gttggcgctc aaatggtact gacgcgggca
     5041 ggcatgccac acattaagca tgcgctgata tttttcccac attggcgtct gggcgttaaa
     5101 accgtgagtt ccggcgtcaa aatgggtata gcgcccttcc acattaacca taaagcggac
     5161 ataaccgagg taacgtgctt cagtggcggc gtcaaagccg aggaaggtga cacgacgttc
     5221 atcgatggat tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat gatacatctc
     5281 cataatgtcg atgatagtgc ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa
     5341 ttcacgcatc tgtaatccgt aacctcgctc aataattgtt tgcaggcgac ggtaacgttc
     5401 agcatttgcc ggatcgagca tagtcatcat cttgtactga ttagacaaaa taagacgttg
     5461 cgcgttggtc atttccattg ttgactcctg tatcactcta ctacggtgaa aaaaaagaag
     5521 gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag
     5581 ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct
     5641 tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa
     5701 accagttaag gattgcaggg ggtaatggcg ttacagaacg cttgcccagc caccacaatc
     5761 cctgcatggg taaacttaag gcgaacagtg cagtggcaac tgccggccca agctgaccgc
     5821 ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat
     5881 aacgcgtcat cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagtg
     5941 gccacgtctt tgagtaatgc tgtccgcggc gaaacaagct aaaaaaatta acagaacgat
     6001 tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa
     6061 attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta
     6121 tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac
     6181 attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca
     6241 tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac
     6301 gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga
     6361 gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc atcatcgatg
     6421 cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg cctgaagcac
     6481 gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc
     6541 acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg
     6601 cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg
     6661 tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc
     6721 acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag
     6781 acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc
     6841 tctacgccct gccttacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg
     6901 gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag
     6961 aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg
     7021 gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc atgggtaccc
     7081 gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg
     7141 ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga
     7201 ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa
     7261 tggacgttta ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg
     7321 gtcgtctgga cgctgttgta ttcactggtg gtatcggtga aaatgccgcg atggttcgtg
     7381 aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg
     7441 ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta
     7501 tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc
     7561 acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag
     7621 aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac
     7681 cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt
     7741 caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt
     7801 gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg
     7861 tctgctttcc agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc
     7921 taacaccaaa gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca
     7981 gtttgcccag tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt
     8041 tatgtctcag ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa
     8101 cagcttcggc ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc
     8161 accggttgat gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc
     8221 caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc ctctgccggt
     8281 tctcggcgct gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg
     8341 ccacctgaat gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt
     8401 cactttctgc gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct
     8461 ggtgacttcc gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg
     8521 cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa
     8581 actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg
     8641 gcagacctct ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgaacg
     8701 tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct
     8761 gactgccact tctgagcgca gccgtcgtct gtctccgcct gcgttccgtt atcagctgac
     8821 tgaacttgcg cgcaaagcgg gcaaacgtat cgtactgccg gaaggtgacg aaccgcgtac
     8881 cgttaaagca gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa
     8941 tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg cagggattga
     9001 aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg aactgcgtaa
     9061 gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg
     9121 tacgctgatg ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac
     9181 cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt
     9241 atcttccgtg ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat
     9301 caacccggat ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc
     9361 tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg
     9421 tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc
     9481 tgacctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa
     9541 atccaaagcg ccgaactctc cggttgcagg tcgcgctacc gtgtttatct tcccggatct
     9601 gaacaccggt aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg
     9661 gccgatgctg cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga
     9721 cgatatcgtc tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaacctcg
     9781 tcatcatccg cagctttgcg ctgcggatat ctgaaccgga aataatcact atttccggtt
     9841 ttttattctc ttaatctgca ttaatccttt ctgattatct tgcttaactg cgctgcatca
     9901 atgaattgcg ccatttcact ttacatactt accactttgt tttgtgcaag ggaatatttg
     9961 cgctatgtcc gcaatcactg aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt
    10021 ggtgattatc ttttttgttg ctattttaac cagccttgcc acctgggtag ttccggtggg
    10081 gatgtttgac agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt
    10141 cgtagatcca cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg
    10201 cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg
    10261 attaacctca ggatcgaaat acgggacagc tgttggcatc atcatgttta tgctggtgat
    10321 tggcggcgcg tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct
    10381 tattcgccat acccgcggga atgaaattct ctttattcct gcgctgttta ttctgttttc
    10441 acttggcggc gcggtatttg gtatgggaga agaggccgtc gcctttgcca ttatcatcgc
    10501 accgctaatg gtccggctgg gctatgacag tattaccacc gtcctggtga cctatattgc
    10561 cacgcaaatc ggttttgcca gttcgtggat gaacccgttt tgtgtggtcg ttgctcaggg
    10621 gattgccggc gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc
    10681 cactatgatt ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct
    10741 tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca
    10801 acgtccgttt acctttggtg actggctggt attgattgtc ctgaccgccg taatggtctg
    10861 ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt
    10921 caccatgggt ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt
    10981 taataccatg gcttcatcct ttaccgaagg gacgcgaatg atgatcgccc ctgccctgct
    11041 ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag
    11101 cgtgttaaat accatcctca acagcattgc caatgccatt agcggtctgg acaacgcggt
    11161 cgcggcctgg tttatgttgc tcttccaggc ggtatttaat ttcttcgtga cgtccggttc
    11221 tggtcaggcg gcgttaacca tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa
    11281 ccgtcaggtt accgtgctgg cattccagtt tggtgatggc ttcagtcaca tcatttaccc
    11341 aacctcagct tcgttaatgg caactctcgg tgtgtgcagg gtggacttcc gtaactggct
    11401 gaaggtgggc gcgaccctac ttggactgct gtttattatg tccagcgtcg tagtgatcgg
    11461 cgctcagttg atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca
    11521 ttctgccgtt tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag
    11581 cgctttcagc gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag
    11641 ccagcaaact tcactgactt catcttcctg tagggcgaag ggaccgtgag agacgcagct
    11701 gaacaacgcg ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc
    11761 aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag
    11821 ttgctcatcg gcctgaacca caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc
    11881 ttttgtctcg gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta
    11941 agttgcacga tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac
    12001 ttcgttctct tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat
    12061 tatcaggaaa ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta
    12121 cggattaatc gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac
    12181 gtcattatcc agcatgccat aactcgccgg attaccgcct ttcggaatac tcaccgagcc
    12241 ggggttgaag tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta
    12301 caccagcaca tcgttctggt ttaaagcagg tagattttcc gggccaaaaa gatgaccatg
    12361 cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa
    12421 atgcagcagc atttgatcca cttcgctgtc gcagttgccg cgcacagcga taaccttatg
    12481 tgccacttca ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt
    12541 acgcgggccg tgattcaaca cgtcgccaag gatcaccagc cactgggcac cgctttgggc
    12601 aaacaactcc agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat
    12661 cagtttcatc actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt
    12721 gctatcagcc agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc
    12781 actggaatgt cgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc
    12841 ggttaatcat tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct
    12901 gacccagtgc taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct
    12961 cggccgttag tggcgctttt ttcgcccccg caaagacaac atccgtcgga cgctcttcgc
    13021 ggatgggcat cagatcgctg cgcagccagg cctgaatccg tcgtgcacgc gcacgatttt
    13081 ctaaatcaag cggataaata cgttcccagg tcggtggcgc aaatcgatct tccagatact
    13141 ccgcaatggc agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc
    13201 gtgtctgacc gtaaccttgc cacgtcggct tcaaatgttc accgctgtcg aggtcgatgg
    13261 tcttgatatg aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag
    13321 gggagaaaaa gtgggcatct gaccaaagcg tgatagcggg tttactcatt atgtcctcgg
    13381 caataaaggg ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata
    13441 ctcacgtaag tcgccgcgct ttctatactt gtggtgttca gatgattaaa caaccggagc
    13501 tgcaatgatc gatctctatt tcgccccgac acccaatggt cacaaaatta cgctgtttct
    13561 cgaagaagca gagcttgatt atcgcttgat taaggtagac ctggggaaag gcggtcagtt
    13621 tcgtccggaa tttttgcgca tttcgcccaa caacaaaatt ccggcaattg ttgatcattc
    13681 tcctgccgat ggcggcgaac cgctaagcct ctttgagtct ggtgccattt tgttgtatct
    13741 gactgagaaa acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca
    13801 gtggttattc tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa
    13861 tcacgcagcc ccccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg
    13921 tctttaccat gtactaaaca agcggctgga aaactcgccc tggctgggag gcgagaacta
    13981 cagcattgcg gatattgcct gctggccgtg ggttaatgcc tggactcgcc agcgaattga
    14041 cctcgcaatg tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac
    14101 cgggcaggca ctgctaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca
    14161 ggttctcgtg tattatttca tcctaagtaa aacaacggag aacctgcaat ggcacaacct
    14221 gccgctatta ttcgtataaa gaaccttcgt ttacgtacgt ttatcggaat taaggaagaa
    14281 gaaattaaca accgtcagga tattgttatc aatgtgacga tccactaccc cgccgataaa
    14341 gcgcgcacta gcgaagatat caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt
    14401 attcagcatg tagagaataa ccgtttctct ttgttggaaa aattaactca ggatgtgctc
    14461 gatatcgcac gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactgcac
    14521 gcgctgcgct acgccgattc ggtatccatg accttaagct ggcagcgtta accgccatat
    14581 cgggaggctg catgaacata gtgatcaccg gagggacggg attaattggt cgccatttga
    14641 ttccgcgttt gctggagctg ggccatcaaa tcacggtagt gacgcgtaac ccacagaaag
    14701 ccagttccgt tctcggccct cgggtgacgc tatggcaagg gctagccgat caaagcaacc
    14761 tcaacggcgt tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga
    14821 ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg
    14881 atttgattaa tgccagcgac acgccgccgt cggtattcat ttccggctcg gcaacgggct
    14941 attatggcga cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta
    15001 ctcataaact ctgcgcccgc tgggaagaga ttgcctgccg ggcgcaaagt gacaaaacgc
    15061 gagtgtgcct gctgcgaacc ggcgtggtac tggcaccgga cggcggtatt ctcggtaaaa
    15121 tgctgccacc gtttcgtctt ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg
    15181 cctggattca tattgatgat atggtcaacg gcattctctg gctgctggat aacgacctgc
    15241 gcgggccatt taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc
    15301 tcggtcaagc gctgcatcgc ccggccattt tgcgcgtccc cgcaaccgcc attcggctgt
    15361 taatgggcga atcttcaata ctggtattag gtggacaacg cgcgctgcct aaacggctgg
    15421 aagaagcggg ttttgcgttt cgctggtacg atttagaaga ggcgctggcg gatgtcgttc
    15481 gctgatgtgg tttacagcaa acatccgcca gttaactccc ggtgttacag gattagtggc
    15541 tttgcgcgat aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctctgtcta
    15601 aacctctatc cagcatctcc tgagcaatac gcagggcttc ttcgtgtttg ccctgcattg
    15661 cgccttcttc acgtaatctg tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc
    15721 gttctgttat ctcaccaata aatgcacgga aacgctgggc atcccctgtt tgtaatacgt
    15781 aattaaacag ggcttttagc tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa
    15841 tttggtcgac taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca
    15901 gcgccatttt gcggtgttgc ataatctcgt catccggcac cacggtaata tccaccaacg
    15961 gaaacgctga tgaatatatt ttgcgggcta tagcaggctc ggcaaattca tcaagccagc
    16021 agagtgaata aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg
    16081 gaagctcttt atagcccgca tcaagatggt tttgcattgc cgcaatggaa taacgcatca
    16141 tgcgaaaagc cattaattct tccagcttac tttggtgctc tatcactaca taaatataac
    16201 ccactccctc ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtctt
    16261 catcaataaa actgtttgat tctagtttaa gcgtcgttaa atcacacagt ttgcgcagcg
    16321 gcgcgggaag atgaatatca ataaaatccc gcgcggtgt
//
LOCUS       DAZPAC010000083        16201 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZPAC010000083 DAZPAC010000000
VERSION     DAZPAC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16201
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.083"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            146..1489
                     /gene="argG"
                     /locus_tag="ACHUPM_002232"
     CDS             146..1489
                     /gene="argG"
                     /locus_tag="ACHUPM_002232"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR0870001.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1497..3123)
                     /locus_tag="ACHUPM_002233"
                     /pseudo
     CDS             complement(1497..3123)
                     /locus_tag="ACHUPM_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(3582..3668)
                     /locus_tag="ACHUPM_002234"
     tRNA            complement(3582..3668)
                     /locus_tag="ACHUPM_002234"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3632..3634),aa:Leu,seq:gag)
     gene            complement(3683..4015)
                     /gene="secG"
                     /locus_tag="ACHUPM_002235"
     CDS             complement(3683..4015)
                     /gene="secG"
                     /locus_tag="ACHUPM_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR0870002.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="ACHUPM_002236"
     CDS             complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="ACHUPM_002236"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR0870003.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5573..6421)
                     /gene="folP"
                     /locus_tag="ACHUPM_002237"
     CDS             complement(5573..6421)
                     /gene="folP"
                     /locus_tag="ACHUPM_002237"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR0870004.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="ACHUPM_002238"
     CDS             complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="ACHUPM_002238"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR0870005.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="ACHUPM_002239"
     CDS             complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="ACHUPM_002239"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR0870006.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9300..9593
                     /gene="yhbY"
                     /locus_tag="ACHUPM_002240"
     CDS             9300..9593
                     /gene="yhbY"
                     /locus_tag="ACHUPM_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR0870007.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9749..10225)
                     /gene="greA"
                     /locus_tag="ACHUPM_002241"
     CDS             complement(9749..10225)
                     /gene="greA"
                     /locus_tag="ACHUPM_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR0870008.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10473..11906
                     /gene="dacB"
                     /locus_tag="ACHUPM_002242"
     CDS             10473..11906
                     /gene="dacB"
                     /locus_tag="ACHUPM_002242"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0870009.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            11961..12068
                     /locus_tag="ACHUPM_002243"
                     /pseudo
     CDS             11961..12068
                     /locus_tag="ACHUPM_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="ACHUPM_002244"
     CDS             complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="ACHUPM_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR0870010.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13280..14245)
                     /locus_tag="ACHUPM_002245"
     CDS             complement(13280..14245)
                     /locus_tag="ACHUPM_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0870011.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="ACHUPM_002246"
     CDS             complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="ACHUPM_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR0870012.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="ACHUPM_002247"
     CDS             complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="ACHUPM_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR0870013.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15220..16191
                     /gene="ispB"
                     /locus_tag="ACHUPM_002248"
     CDS             15220..16191
                     /gene="ispB"
                     /locus_tag="ACHUPM_002248"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR0870014.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
BASE COUNT         4137 a         4329 c         3775 g         3960 t
ORIGIN      
        1 agcgcattat tttacaaaaa acacactaaa ctcttcctgt ctccgataaa agatgattaa
       61 atgaaaactc atttattttg cataaaaatt cagtgagagc ggaaatccag gctcatcatc
      121 agttaattaa gcagggtgtt attttatgac gacgattctc aagcatctcc cggtaggtca
      181 acgtattggt atcgcttttt ccggcggtct ggacaccagt gccgcactgc tgtggatgcg
      241 acaaaaggga gcggttcctt atgcatatac tgcaaacctg ggccagccag acgaagagga
      301 ttatgatgcg atccctcgtc gtgccatgga atacggcgcg gagaacgcac gtctgatcga
      361 ctgccgcaaa caactggtgg ccgaaggtat tgccgctatt cagtgtggcg catttcataa
      421 caccaccggc ggcctgacct atttcaacac gacgccgctg ggccgcgccg tgactggtac
      481 catgctggtt gctgcgatga aagaagatgg cgtgaatatc tggggtgacg gtagcaccta
      541 taaaggaaac gatatcgaac gtttctatcg ttatggtctg ctgaccaatg ctgaactgca
      601 gatttacaaa ccgtggcttg atactgactt tattgatgaa ctgggcggcc gtcatgagat
      661 gtctgaattt atgattgcct gcggtttcga ctacaaaatg tctgtcgaaa aagcctactc
      721 cacagactcc aacatgcttg gtgcaacgca cgaagcgaag gatctggaat acctcaactc
      781 cagcgtcaaa atcgtcaacc caattatggg cgtgaaattc tgggatgaga gcgtgaagat
      841 cccggcagaa gaagtcacag tacgctttga gcaaggtcat ccggtagcgc tgaacggtaa
      901 aacctttagc gacgacgtag aaatgatgct ggaagctaac cgcatcggcg gtcgtcacgg
      961 cctgggcatg agcgaccaga ttgaaaaccg tatcatcgaa gcgaaaagcc gtggtattta
     1021 cgaagctccg gggatggcac tgctacatat cgcttatgaa cgtctgttga ccggtattca
     1081 caacgaagac accattgagc agtatcatgc acatggtcgt cagttgggcc gtctgctgta
     1141 ccaggggcgt tggtttgatt cccaggcgct gatgctgcgt gactctctgc aacgctgggt
     1201 tgccagccag atcactggtg aagttaccct ggagctgcgc cgtgggaacg attattcaat
     1261 cctgaatacc gtctcagaga acctgaccta caagccagag cgtctgacga tggaaaaagg
     1321 cgactcggtg ttctcgccag atgatcgtat tggtcaattg accatgcgta acctggatat
     1381 cactgatacc cgagagaaac tttttggtta tgccaaaact ggcctgctct cctcctctgc
     1441 cacttcaggc gtgccgcagg tggagaatct ggaaaacaaa ggccagtaat tcgacatcaa
     1501 ccctgccctt caggggtggg gttaccttgc ttatcctctg taaactcctt tctgagcatc
     1561 acataatcga aaacatcatg gtttgcatcc acaacccgtg agtctccttt atagtgtgca
     1621 atcacttcct ccagcgtttt cggttgttcc ggctttgtta cccccatcca cgcattaata
     1681 aggtaattat tgtaagctgt cgaaaagatg ttgttttccg ttgtacgatc cacgccatcc
     1741 ccaagaacgg ggctatacca gataaacatc gggacatgat atgcctgctg gctagcctcc
     1801 ctgcctccat gaaaatagac gttcttcttc gtagggtcac gttccaggcc gtggtcggca
     1861 aaatacatga ccgaggcgcg gcgatctttt aataattcaa aaacctgacc tagcaaacta
     1921 tctgtgtaat gaatggagtt gtcatagcag gcatcctgat cgtcctgcgg ttgaaacacg
     1981 gcgctggatt gcggataggc gctacaagcc ggttcatggc ttccatttaa atgaagaaca
     2041 atcagtttct tttgctgcgt attttgctgt aatgcttgcg ataaatgcgg caacaacaat
     2101 tcatcaaatc ctctgacata aactgtttcc atggcgcgca tggcgatact ggtaactgct
     2161 gtaccattct gccgaaaagc ggattgcgag cttagccaga aagtctgaaa tcctgcttga
     2221 ttagccatat taataatgtt gtcggggtaa ttatgaatgt catgactcaa aacagaatca
     2281 gcagttaaag aaaggggaac cgacagcgca gtgtaaggtg cgccgcttat tgattgatta
     2341 aacagtttga tctgttttct ttgtgcttca acttgcggtg tcgtagagcg tgtatatcca
     2401 tacaaagaca tattgtcgac acgtacagac tcccccacaa tcaacacgta ggtatcaata
     2461 cctgtatccc tgactgataa ttgaaaatac ggcaccgtgt ttgcaattga gagtaatctt
     2521 tgatgctctt tcgctgctaa agcaaaataa ttgagattga aaaacggcgt ataggtagca
     2581 aatcgcgacg ctagtatata tggactgaat gcttttttat tttttgcatc tttataagca
     2641 aattgacaag cagaaaataa actgcccgaa atgacaatca gcaataatat tcctgtcact
     2701 ttttttgtcg gcaaggaaac atcatatttt attattactg ccaaaaaaag aagggataaa
     2761 aaagcaaaag cacatagata agggatatac atccccagca ttttgacaac ttcatccgga
     2821 tcactctgca gcacactaat cgcaaatcca tcattgaaag ttgtaccaaa ggtacaccat
     2881 gaatagaggc taatactcat gtctgccgca gtcagtaaca caaatggtgc ggcaacaagc
     2941 aggcgaagcg gaagggatga tatacgtttt acaaccagga tggtcaggac gaagaacagc
     3001 aatcgctgaa ccatatgcgc gccggaagag gcgactaagt tcgtaatacc aaaaacaata
     3061 acacaaagat acaacaacca atgagattta aaatcttcta gcaaatttgt tgaatactgt
     3121 catccagaaa tccaagttca caaccaatat aaacaattat caacaatgtt caattttata
     3181 tcagacatgt ttttcaactc tgatccatat cagaccgaaa cactgttagt tttttctttt
     3241 taaatgatgc ggccattgcg ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac
     3301 attacgcaga acgtagccat cagtagttat actgacagga gcaggatggt ttatcgagaa
     3361 gtaaacatat ccgtattcgt accggaactg gagttgacac aataaagtgc caattatgtc
     3421 agcagaaggg aaaagtttat aactaaggcg taccggcacc atcgtttcaa ggtaccagct
     3481 acgagtaaag caactggacg agatacagat acctgacaac cattcctcag accaggacca
     3541 aaacgaaaaa agacgctttt cagcgtctct tttctggaat ttggtaccga ggacgggact
     3601 tgaacccgta agccctattg ggcactacca cctcaaggta gcgtgtctac caattccacc
     3661 acctcggcac ggatactact ttttagttcg ggatatcgct ggtcggctta gccggagcag
     3721 ctggctgagt ttgttcggtt ttcgccggtg cactcagatt ttcccattcg ctacctttat
     3781 tggttttgtt gctattgatg ttacccagca ccagactgat gataaagaat aacgttgcca
     3841 gcagcgccgt catgcgggtc atgaagttac cagaaccact tgaaccaaac agcgtagcgg
     3901 aagcgcctgc tccgaaggag gctcccatat cagcgccttt accttgctgc agcatgatca
     3961 gaccaacaag gccaattgcc acaataagga aaactaccaa aagagcttcg tacataatca
     4021 acctgttcct tgcggagttg ccgcgtacca atgcttcaac caataaagcg ggagttttta
     4081 atctttccca ctgaagtggg tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa
     4141 ttttatcgca ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt cgctgagaaa
     4201 aaaggcgaca atcgccgcct ttttagccag ttatttaccg ttttaaacgg cttttactgc
     4261 atcggcgata cggtgtgcaa attcagttac ctgcgcttcg tcttcacctt ctaccatcac
     4321 gcgaattaac ggttcggtgc cggatttacg caacagcaca cgtccacggc tacccagcgc
     4381 cgcttcaact tcggcggtca cagctttaac tgactcatgc tcaagcggat cgccgctacc
     4441 tgcggtgtaa cgcacgttaa ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc
     4501 gtgcaggctc atatggttac gtgccatcgc cgccagcacc tgcaagccag caacgatgcc
     4561 gtcaccggta gtggttttat ccagcaggat cacatgaccg gaattctctg caccgatacg
     4621 ccagcctttc tcctgcattt tttccagtac gtagcggtca cccactttcg cgcgcgcaaa
     4681 tggaattcct aactgtttca acgccagctc aagccccatg ttactcatca atgtacccac
     4741 agcaccacca cgcagctggc cctgacgaag accttcacgc gcgatgatat acatgatctg
     4801 atcaccatcg actttattgc cttcatggtc aaccataatc acgcgatcgc cgtcgccgtc
     4861 gaaggcaata ccgagatccg ctttttcagc cagcacacga gcctggagcg cgcgaacgtc
     4921 ggtagccccc acttcggcat tgatgtttac accgttcggc tcacaaccga tagcgataac
     4981 gttcgccccc agttcacgca gcacgttcgg cgcgatgtga taagtcgcac cgtttgcgca
     5041 atccaccaca atcttcagtt cgctgaggct aagttcgttc gggaacgtgg ctttgcaaaa
     5101 ctcgatatag cgacccgcag catcaacgat acggctggct ttacccagtt ctgccgaatc
     5161 aacacagctg atctcttttt ccatttccgc ttcgatggcc tcttctaccg catccggtag
     5221 tttggtgccg tcgatagaga aaaatttaat gccattatcg tagaacgggt tatgcgatgc
     5281 agatatcaca attccggcct ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt
     5341 tggcatcggg ccagtgaaga gtgcggaaag gcccgctgcc gccagacccg cttccagtgc
     5401 tgactccagc atatagccag aaatacgcgt gtctttacca ataatgatct tacgggagcc
     5461 gtggcgcgcc agcactttac ccgcggccca acccagctta agcacaaaat caggtgtgat
     5521 cggcgcatcc cctacacgac cacgaatccc atcggtaccg aaatatttac gattactcat
     5581 agcgtttgtt ttcctttgca gacagagtgg cttccaccac ccgcatcgct tctacggttt
     5641 ctttgacgtc atgaacacga ataatgtgcg cgccttgcat tgcggcaatg accgcacagg
     5701 ccagactacc gctcaggcgc tcggacggcc ccacgttcag cagctgccca atcatcgatt
     5761 ttcgtgacat acctaccaac agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca
     5821 gtaatgaata gttatgggag agatttttac cgaaaccgaa tccggggtcg agcaacaatt
     5881 tctcttttgc gatacccgcc tgctcgcaac gtgctatttg ctcaataaag tagcgattca
     5941 cttctgcaaa gacatcgtca tacttcggag cttcctgcat ggtttttgga tttccctgca
     6001 tatgcatcag acaaaccggt aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg
     6061 aaagggagcg gatatcgtta ataatgtgag cgccaacttt cgctgactca cggatgactt
     6121 ctggtttgga tgtatctacc gagatccaga cttcgaagcg ttgagcaatt gcctcaacca
     6181 caggaataac acgttgcaac tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg
     6241 actcgccacc aacgtcaatg atcgtcgcgc cagcgttgat catcagattc gcatgtttca
     6301 ccgcatctat cagcgagtta tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga
     6361 ggatccccat tacgtgagga tggctaaggt ccagtgaagt gccctgggca aagagtttca
     6421 tggtgttatc cctggtatga attgataaga aaaaaccccg gagcacgccc cggggttttc
     6481 ggtacaaata cagtcatctg atgcgggaac ttacttgtcg cctaactgct ctgacatggt
     6541 gttacccggg ttcggcgtac gcggttcatc aaccggacga ggagcctttg gactaccatt
     6601 gtcgccagaa ttgttagaag cgcctggttc ttcccagccc gctggcggac gtacatcgcg
     6661 acgtgccatc aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca tgagagcatc
     6721 tttcatcgca tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac gattatagtt
     6781 acgctcaatc agtgctttca cttcctggtc gatgatacgt gcagtttcat cggacatatg
     6841 tttcgctttc gctacgctac ggccgaggaa cacttcacct tcttcttccg cgtacagcag
     6901 tggacccaat ttctcagaga agccccactg agtcaccatg ttacgcgcca ggttggtcgc
     6961 aactttaata tcgttggacg caccggtaga aacatgttcc ggcccgtaga tgatctcttc
     7021 tgccagacga ccaccgtaca gcgtagaaat ctggctttcc agtttctgac ggctggcgct
     7081 gattgcgtcg ccctcaggca agaagaaagt cacacccagc gcacgaccgc gtgggataat
     7141 cgtcactttg tgcaccggat cgtgttccgg caccaggcga ccgataatcg catgacccgc
     7201 ttcgtggtaa gccgtcgatt ctttctgcgc ttccgtcatc accatggagc gacgttccgc
     7261 acccatcatg attttatctt tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt
     7321 gccacgagca gcgaacagtg ccgcttcgtt taccaggttc gccaggtcag caccggagaa
     7381 accaggagta ccacgggcaa tgattgccgc gtcgatatcg ggtgccaatg gtacgcgacg
     7441 catgtgaact ttcaggatct gctcacgacc gcgaacatct ggcaagccaa ccacaacctg
     7501 acggtcgaaa cggccaggac gcagcagggc cgggtcaaga acgtccggac ggttagtcgc
     7561 ggcgataacg atgatacctt cgttaccttc gaagccatcc atctcaacca gcatctggtt
     7621 cagagtctgt tcacgttcat cgtgaccacc gcccagacca gcgccacgct ggcggcctac
     7681 ggcgtcgatt tcatcgataa agatgatgca cggtgccgct ttcttcgcct gttcgaacat
     7741 gtcacgaaca cgggatgcac ccacaccgac gaacatttct acgaagtcag aaccggagat
     7801 agtaaagaac ggaactttcg cttcgcctgc aatcgctttc gccagcagcg ttttaccggt
     7861 acccggagga ccgaccatca ggacgccttt cgggatctta ccgccgagtt tctggaagcg
     7921 gctcggctcg cgcagatatt caaccagttc agcaacttct tcttttgctt cgtcgcagcc
     7981 cgcaacgtca gcaaaggtcg ttttgatctg atcttccgtc agcatgcgcg ctttgctctt
     8041 accaaacgac atggcacctt tgccaccgcc gccctgcatt tgacgcatga agaagatcca
     8101 cacaccaatc agcaacagca tcgggaacca ggagatgaag atagaagcca gcaggcttgg
     8161 ttcttcaggc ggttcaccga caaccttgac gttcttggtc aacaggttat ccagtaattt
     8221 cggatcctga accggaatgt aagtggtata acggttacta tctttcttgg taacgttgat
     8281 ttcacgtccg ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt
     8341 agagtaatcc accttacggc cattagactc gctgggccca aagctctgga atactgacat
     8401 cagcacaacg gcaatgacca gccagagtat taggtttttc gccatgtcac tcaagggatt
     8461 aacctcttat tacaactgtg ttaaaaacag cgtcaggata ctctatatcc agcatctttc
     8521 aaactttcgt ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa tatacacttc
     8581 ccgcgaacgt gcacgagaag agtccggctt acgaactttg accttcgtaa acagggagcg
     8641 aatttccctt agatactcat cgaaaccttc gccctggaac accttcacta caaaactgcc
     8701 acctggcgct aatacatcac gacacatttc tagcgccagt tccaccagat acatggcacg
     8761 ggggatatcc accgccggtg ttccgctcat gtttggtgcc atatcggaca tgacaacctg
     8821 gactttgctg tcgccaacgc gctccagcag tgctttcatc accagttcat cacgaaaatc
     8881 gccctgaaga aagtctacac caacgatagg atccataggt aaaagatcgc aagcgatgat
     8941 gcggcctttg ccgccaattt gggtgaccac atattgtgac caaccacccg gagcagcacc
     9001 aaggtcgaca accgtcattc ccggtttaaa gagtttgtca ctttgctgta tttcatcaag
     9061 tttaaaccag gcacgggaac gtaacccctt tttctgtgcc tgttgaacat atttatcgct
     9121 aaagtgttcc tgaagccagc ggctggagct ggcagaacgc ttcttacctg tcatttaact
     9181 ttcccatggg gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga
     9241 tatatgggag atggcggtag aatgacccat tttcaatccc aacgtaagca aaaaatacga
     9301 tgaatctgag tactaaacaa aaacagcacc tgaaaggtct ggcacatccg ctcaagccag
     9361 ttgttctgct tggcagtaat ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt
     9421 tagagcacca tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa acaaaaacct
     9481 tgatcgtgga agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa
     9541 cgctggtgct ttatcgccca actaaagaac gtaaaatctc gctgccacgc taagattatc
     9601 ctaaagttac acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa
     9661 tgccacgctc tgttcgttga taaaaggccg catagcggcc tttttccttt ctttacaata
     9721 catcaacatc ttgagtattg ggtaattctt acaggtattc caccttaatt acttcaaatt
     9781 ctacttcgcc gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc
     9841 cacgagcaat aggcgagttt acagaaatca ggttttgttt aaagtcagct tcgtcatcgc
     9901 caacgatgcg ataagtctgt tcttcgtcag aatccagatt cagcaccgtt acggtagcac
     9961 caaaaataac gcgcccattg ttaggcattt tggtgacatc aatcacctgc gcgtttgaca
    10021 gcttggcttc gatgtctttg atacggcctt cgcagaagcc ctgctgttca cgagctgcgt
    10081 ggtattcggc gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga
    10141 tttcaggacg gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc
    10201 gtaaggtcat cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag
    10261 tcttcaccca ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca
    10321 aaaaaatacc gacccaggta caagtcccag gtcagctaca attcacattt tgatagtcat
    10381 tttaccctga agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg
    10441 acggctcgat tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat
    10501 tgaccagctg tatagcgttc agtgttcagg ccgcaaatgt tgatgagtac attactcaac
    10561 tccccgctgg tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta
    10621 ttgattacca cagtcagcag atggcgctgc ctgccagtac ccagaaagtg attactgcgc
    10681 tggcggcgtt gattcaactc ggccccgatt ttcgttttac cacgacgctt gaaaccaaag
    10741 gcaatgtgga aaacggcgta cttaagggtg acttagtggc gcgatttggt gccgatccga
    10801 cgttaaaacg tcaggatatt cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc
    10861 aaatcgatgg caatgtgttg atagatacct ccattttcgc cagccacgat aaagcccccg
    10921 gctggccatg gaatgacatg acacaatgct ttagcgctcc gcctgccgcc gccatagttg
    10981 accgcaactg tttctccgtc tcgctctaca gtgcccaaaa acccggtgat atggccttta
    11041 tacgcgtggc atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgtg
    11101 gttctgccga agcgcaatac tgcgaactgg atgtcgtacc aggcgacctg aaccgcttta
    11161 cgctgacggg atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg
    11221 atggagccag ttatgccggt gcaattctga aagatgagtt aaaacaggcg ggtatcacct
    11281 ggagcggaac actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta
    11341 aacagtcggc cccgctgcac gatctgctta agattatgct gaaaaagtcg gataacatga
    11401 tcgccgatac ggttttccgc atgataggcc atgcgcgctt caatgtacct ggtacatggc
    11461 gggccgggtc ggacgctgtg cgtcagatcc tgcgccagca agccggtgtc gatattggaa
    11521 acaccattat tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca
    11581 tgatgcaggt gctgcaatac attgcccaac acgacaatga acttaacttt atctccatgc
    11641 tgccgctggc gggctatgac ggctctttgc agtaccgtgc aggtctgcat caggcgggcg
    11701 tggatggaaa agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat
    11761 tcattaccac agcgagcggg caacgaatgg cgtttgtgca atatctttct ggctatgcag
    11821 tagaacctgc ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt
    11881 ataaagatat ttatcagaac aattagtcaa aaagaaaccc cggcacatgg ccggggcttc
    11941 agattattga caaagtgcgc tttgtttatg ccggatgcgg cgtaaacgcc ttatccggcc
    12001 tacaaaatcg tgcaaattca acatattgca attctcttgt aggcctgata agcgtagcgc
    12061 atcaggctga tttggcgttt atcatcagtg attaacgctt gtaaatgaac tcaacgcctt
    12121 cttcgtcgtc ttcgtcccag tcgtcatccc agtcttcatc atcctcttca gcaatctctt
    12181 caagctgctg gcgatgataa tcgtcccaca tgaattcgac tttctctggc tgtttcgctt
    12241 cttcagcctg cacgaccggg ttttcaagga taaaggtcat cacatcccag cagagatctt
    12301 tcacgcccag tccgctcgcc gcagagatca gataatattt atcttcccag cccagcgctt
    12361 cagcaatcgc tttcgctttc tcttcagctt ctgccttatc cagcagatcg atcttattga
    12421 acactaacca acgcggtttc gccgccagat cctggctgta tttttccagc tcgctgataa
    12481 taatacgcgc gttttcaacc ggatcggtgc cgtcaatcgg atcgatatcg atgaggtgca
    12541 acaggacgcg gcaacgttcc aggtgcttca ggaagcggat acccagacct gcgccttccg
    12601 cagcgccttc aatcagtcct ggaatatcgg caacaacgaa gctcttttcg ttgtccattc
    12661 gtaccacacc cagacttggc accagagtgg taaacggata atccgccact ttcggtttag
    12721 ccgccgatac cgcgcgaata aaggtcgatt tacccgcgtt tggcatcccc aacataccga
    12781 cgtcagccag cagcatcagc tccagcagca gctcacgctt atcacccggc gtaccgttgg
    12841 ttttctgccg tggtgtacgg ttaacggacg atttgaaacg ggtattgccc agaccgtgcc
    12901 agccgccctt agcaaccagc agacgctgac cgtgtttagt catatcgccc atggtttcac
    12961 cagtaccctg gtcgattaca cgcgtaccta ccggcacttt aatcgtcacg tctttaccgc
    13021 gcttaccggt acaatcgcgg cttgcgccgt tctgaccgcg ctctgcacgg aaagattttt
    13081 caaaacgata atcgataagc gtgttcaggt tttcgtcggc ttccatccat acgtcaccac
    13141 catcaccacc gtcgccgcca tccgggccgc ctttcggaat atacttttcg cggcggaagc
    13201 tcacgcaacc attaccgcca tcacctgcaa cgaccagaat cgatgcttca tcaacaaact
    13261 tcattttatt ctccataaat cattcgcctg cgcggggttg cgataccacc gttgtatgct
    13321 tacgtaatcc gccccaaata cgatgaccaa tggcggaata catcgcgccc gcaaccacga
    13381 caaacgcacc gagataacct aaaaggttta acatcggtct ggcgaagaaa tcgggccagg
    13441 ccagtgataa aagatctgaa aaaaacagcg taaacagtgg ggtgagcgtg atgatcgcgc
    13501 tcacctgcgc tgcctgccag cgagccatcg cttccgccag ggcgccatat cctaccaatg
    13561 tattcagtcc gcaaaaaatt aaacatgcga gctgccagtg gctaagctgc gctatcactc
    13621 caggctttgc cagagggaag agcgcaattg tacataaagt gtacagtaaa aacaggatct
    13681 gcggtgaggc cagccgacgc aataaaacct tttgcgccac gccataactc acccaaaccg
    13741 tcgccgcacc gaccccaaag ataactcccc aggtgtaatc ggtgagcttt gtaaatatct
    13801 cgaccagact ggtgttaaaa aacatcacca ggccgctcag gagcatcaat gcccctacaa
    13861 cctgagtgct gcgcattttc tctttcagga taaatacgct ggcaaccatc atgccaactg
    13921 gcgagagttg cccaatcacc tgcgaagcgg tcggactcag gtattgcaag gatgagctga
    13981 acaggatgaa gttcccaaac agcccggcgg tcgccactgc caacaaaatc aaccagcgtg
    14041 gcttacgaaa cacgcgtaat ggcggcaacc tcttcttcac cgcaagaatg gcaccaaggc
    14101 caatactcgc catcaagaaa cggtaaaaca cgattgtcgg aggttccatc acctccagca
    14161 cctgcttcat tgcgattggc aacgcccccc agcaaattgc tgtggtgagc gccaaaagaa
    14221 tgccaatgcc tgcctgctgc ttcataccgt attccctgca aagcgcattt gcggattaac
    14281 gtcgcaccat gcgcattttt accggttatc gaatgtaaaa agccccgcaa cgtgttgcgg
    14341 ggctttcatc cgttaccggg acgcgaaaaa cttattcagc ttcgatgctg ataaatttac
    14401 ggtttttcgg gcctttaact tcgaatttca ctttaccgtc tgctttagca aacagagtgt
    14461 ggtcacgacc gcaacctacg ttagcgccag cgtggaattt ggtaccacgt tgacgaacga
    14521 tgatgctacc cgccagaacg gattcgccac cgaaacgctt aacgcccagg cgtttagctt
    14581 ctgaatcgcg accgttacgt gtggagccgc cagccttttt atgtgccatt tgaaatctct
    14641 cctcaggtct taggcgctga tgccagtaat tttcacatca gtgaaccact gacgatgacc
    14701 ctgctgctta cgatagtgtt tacgacgacg aaacttaacg attttaactt tctcgccacg
    14761 accgtgagca acaacttcag ctttgattac gccgccatca acgaaaggaa cgccgatttt
    14821 gacttcttca ccgtttgcga tcatcagcac ttcagcgaac tcaacagttt cgccagttgc
    14881 gatgtccagc ttttccaggc gaacggtctg accttcgctt actcggtgtt gtttaccacc
    14941 actttggaaa accgcgtaca tataaaactc cgcttccgcg cacacctttt caatgattca
    15001 gagtgcgcta taaatattca caatagggcg cgaatattac gcaaaacgca cgcctttgac
    15061 aagtgctaca gtcaatacac gaagaaaaaa aacacaactt gtacggtaac gtttatctgt
    15121 gccatttttt cagtacaatc accctatatt cctaaccata aaccctaagt tgcctttgtt
    15181 cacagtaagg taatcggggc gaaaagcccg gcttttgcga tgaatttaga aaaaatcaat
    15241 gagttaaccg cgcaagatat ggcgggtgtt aatgcggcaa tccttgagca gcttaattcc
    15301 gacgtccaac tgatcaatca gttaggctat tacatcgtca gcggcggcgg taaacgtatt
    15361 cgtccgatga ttgctgtact ggctgcacga gctgttggct atgagggaaa tgcgcatgtc
    15421 accattgctg ccctgatcga gtttatccac acggcgactc tgctacacga cgacgttgtg
    15481 gatgaatcag atatgcgcag gggtaaagct accgccaacg ccgcatttgg caatgccgcc
    15541 agcgtgctgg taggcgattt tatttatacc cgcgcgttcc agatgatgac cagcctcggt
    15601 tcactcaaag tgctggaagt catgtcagaa gctgtaaacg tcatcgcaga aggtgaagtt
    15661 ctgcaactga tgaacgttaa cgatccggac atcactgaag aaaactacat gcgcgttatc
    15721 tatagcaaaa ccgcgcgtct gtttgaggct gccgcgcagt gttccgggat tctggctggc
    15781 tgtacgccgg aggaggagaa aggcctgcag gattatgggc gctatctcgg cactgctttc
    15841 cagttgatcg acgatttact cgattacaat gccgatggcg aacagttagg taaaaatgtc
    15901 ggcgacgatc tgaacgaagg taaaccgacg ctgccgctgc tgcatgcgat gcatcatggc
    15961 acaccagaac aggcacagat gatccgtacc gccatcgaac agggtaacgg tcgccatctt
    16021 ctggaaccgg ttctggaagc aatgaacgct tgtggatctc ttgaatggac gcgtcagcgt
    16081 gccgaggaag aagcagacaa agccatcgca gcattacagg tgctcccgga caccccttgg
    16141 cgagaagcac tcatcggcct cgcgcacatc gctgttcaac gcgatcgtta atcccctccc
    16201 t
//
LOCUS       DAZPAC010000084        15961 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZPAC010000084 DAZPAC010000000
VERSION     DAZPAC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.084"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHUPM_002249"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHUPM_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR0870015.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHUPM_002250"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHUPM_002250"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR0870016.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHUPM_002251"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHUPM_002251"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFR0870017.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHUPM_002252"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHUPM_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHUPM_002253"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHUPM_002253"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR0870018.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHUPM_002254"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHUPM_002254"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFR0870019.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="ACHUPM_002255"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="ACHUPM_002255"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR0870020.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="ACHUPM_002256"
     CDS             8229..8939
                     /locus_tag="ACHUPM_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0870021.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHUPM_002257"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHUPM_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR0870022.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="ACHUPM_002258"
     CDS             9636..10343
                     /locus_tag="ACHUPM_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0870023.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="ACHUPM_002259"
     CDS             10343..10891
                     /locus_tag="ACHUPM_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR0870024.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="ACHUPM_002260"
     CDS             10901..12067
                     /locus_tag="ACHUPM_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0870025.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="ACHUPM_002261"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="ACHUPM_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
     gene            13576..14229
                     /locus_tag="ACHUPM_002262"
     CDS             13576..14229
                     /locus_tag="ACHUPM_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0870026.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHUPM_002263"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHUPM_002263"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFR0870027.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15961)
                     /locus_tag="ACHUPM_002264"
     CDS             complement(15612..>15961)
                     /locus_tag="ACHUPM_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0870028.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3700 a         4345 c         4162 g         3754 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttac catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaaac ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatagc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctggatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag ctcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccagccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 a
//
LOCUS       DAZPAC010000085        15652 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZPAC010000085 DAZPAC010000000
VERSION     DAZPAC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15652
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.085"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..1411
                     /locus_tag="ACHUPM_002265"
                     /pseudo
     CDS             1..1411
                     /locus_tag="ACHUPM_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
     gene            complement(1499..2470)
                     /gene="lpxM"
                     /locus_tag="ACHUPM_002266"
                     /gene_synonym="msbB"
     CDS             complement(1499..2470)
                     /gene="lpxM"
                     /locus_tag="ACHUPM_002266"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0870029.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2590..3912)
                     /gene="mepM"
                     /locus_tag="ACHUPM_002267"
     CDS             complement(2590..3912)
                     /gene="mepM"
                     /locus_tag="ACHUPM_002267"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR0870030.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3928..4860)
                     /gene="znuA"
                     /locus_tag="ACHUPM_002268"
     CDS             complement(3928..4860)
                     /gene="znuA"
                     /locus_tag="ACHUPM_002268"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR0870031.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4939..5694
                     /gene="znuC"
                     /locus_tag="ACHUPM_002269"
     CDS             4939..5694
                     /gene="znuC"
                     /locus_tag="ACHUPM_002269"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR0870032.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5691..6476
                     /gene="znuB"
                     /locus_tag="ACHUPM_002270"
     CDS             5691..6476
                     /gene="znuB"
                     /locus_tag="ACHUPM_002270"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR0870033.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6623..7633)
                     /gene="ruvB"
                     /locus_tag="ACHUPM_002271"
     CDS             complement(6623..7633)
                     /gene="ruvB"
                     /locus_tag="ACHUPM_002271"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR0870034.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7642..8253)
                     /gene="ruvA"
                     /locus_tag="ACHUPM_002272"
     CDS             complement(7642..8253)
                     /gene="ruvA"
                     /locus_tag="ACHUPM_002272"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR0870035.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(8392..8457)
                     /gene="yobI"
                     /locus_tag="ACHUPM_002273"
     CDS             complement(8392..8457)
                     /gene="yobI"
                     /locus_tag="ACHUPM_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFR0870036.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8530..9132
                     /locus_tag="ACHUPM_002274"
     CDS             8530..9132
                     /locus_tag="ACHUPM_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR0870037.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(9134..9655)
                     /gene="ruvC"
                     /locus_tag="ACHUPM_002275"
     CDS             complement(9134..9655)
                     /gene="ruvC"
                     /locus_tag="ACHUPM_002275"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR0870038.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9690..10430)
                     /locus_tag="ACHUPM_002276"
     CDS             complement(9690..10430)
                     /locus_tag="ACHUPM_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0870039.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(10459..10911)
                     /gene="nudB"
                     /locus_tag="ACHUPM_002277"
     CDS             complement(10459..10911)
                     /gene="nudB"
                     /locus_tag="ACHUPM_002277"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR0870040.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(11029..12801)
                     /gene="aspS"
                     /locus_tag="ACHUPM_002278"
     CDS             complement(11029..12801)
                     /gene="aspS"
                     /locus_tag="ACHUPM_002278"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR0870041.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            13111..13677
                     /locus_tag="ACHUPM_002279"
     CDS             13111..13677
                     /locus_tag="ACHUPM_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR0870042.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            13674..13769
                     /locus_tag="ACHUPM_002280"
                     /pseudo
     CDS             13674..13769
                     /locus_tag="ACHUPM_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14032..14280
                     /locus_tag="ACHUPM_002281"
     CDS             14032..14280
                     /locus_tag="ACHUPM_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0870043.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            14881..>15652
                     /locus_tag="ACHUPM_002282"
     CDS             14881..>15652
                     /locus_tag="ACHUPM_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0870044.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSH"
BASE COUNT         3970 a         3968 c         3601 g         4113 t
ORIGIN      
        1 cagtgagttt gatgaaatat ggaaagcgat gctgaccttg caattccatg acatccttcc
       61 cggctcatgt atttcaagag tttatcatga aactgaaaag gaatatctta aactagaagc
      121 aaaaaccgag aaaattattt ctgatgcgca gtctacttta ttaagtaaaa ttgacacttc
      181 ttcttataaa gatccgcaca ttttgtttaa taccacctgt tttgctcgta acgaatggat
      241 caatataaac aacaactggc tcaaagcacg agtaaactct tatggttatg ctgtgattgt
      301 tccaaagaat aagatcgtaa atggtctgaa ggcagaatct caaagtattg aaaataatta
      361 cattaaactt cttttttcag aaagcggtga tttaatttcg ctatatgata aaagatatgg
      421 taaagaatac attactgaaa atatgcacag tgagatccgt gcataccacg aagatgcagg
      481 ttttttcgcg gcctgggatt ttgcttcaaa ttatcgtgat ggggaatcat atgttttact
      541 cgctgaaaaa atgacaactg tgatcagcgg gcctaaaact acgatgacgc tgatttatca
      601 ctacaattcc tcttatcttc gtttagcctt taccctgaca caggatagtc cacgcgttga
      661 tgtccaaacg tttattgatt ggcacgaacc taatgtttct ctcaaagtaa aattcccggt
      721 gtcagtacaa acatctcttg cacaatgtca aattcagttc ggtgtaattg acagaccaac
      781 gcatagcgat gattcgttcg cttttgccaa agatgaaata ccagcacatc actgggtcga
      841 cctttctgat cgagaaactg gcatagcttt aatagcaaaa aacaaatatg gctatcgtgt
      901 caaagaccag acgcttgaat taactttgct gcgttcgcag cataaacctg gggaagttgt
      961 taagacagac aattatgata atttcctgat taataatttt gcagatattg gtaaacaaaa
     1021 attcatattc tccttatttc ctcatcatgg agattaccgt gtgggtggtg ttattaatca
     1081 agcctatata atgaatcatc ccttacaggt cgtccctgtc aaaccgcacc cgggtgaatt
     1141 acccccttca ctatcatttt ttgctatcga tactaacagc gttatcatcg aaacaataaa
     1201 attagctgaa gacggcgacg gtataatcgt aagattatat gagagtcatg gattagaaat
     1261 ttcagccatg ctttcgtgag tatgtaatta tcactacgtt caatatgtcg atcttcaaga
     1321 aaataagtta gatgacagtt tttattgcaa tcaatattgc aatcttgagt tcaaaccttt
     1381 tgaaattatc acgctacgat taactcaata gcgtactaac ctaaaaactc aaaatagtgg
     1441 gaacactcat tgagattttt tatcgagtac agcctctcaa taatttgaga ggcttttttt
     1501 atttgatagg ataaagatct ttacgcttat acggttggat ttcgcccggt ttgcgagttt
     1561 tcagcaattt taatatccag gtgtattgtt ctggtcgcgg accaacaaaa atctcgactt
     1621 cttcattcat ccgccgcgca atcgtatgat catccgcctc taacagatca tccatcggtg
     1681 ggcgcacctg aatcgtcaga cggtgcgtct tgccatcata aatcggaaac agcggaacaa
     1741 cgcgcgcacg gcacactttc atcaaacgac caatcgcggg caacgtcgct ttataggtgg
     1801 caaagaaatc cacaaattcg ctgtgctctg ggccatgatc ctgatcgggt aaataatatc
     1861 cccagtaccc ctgacgtacc gactggatga atggtttaat accgtcattt ctcgcatgca
     1921 gacgaccgcc aaagcgacga cgcaccgtgt tccagacata atcaaaaacc gggttgccct
     1981 gattatggaa catcgctgcc attttctgcc cttgcgaggc catcagcatg gcaggaatat
     2041 cgacggccca accgtgcggc accagaaaga taactttctc gttattacgc ctcatctctt
     2101 cgatgatctc cagcccttgc cagtcaacgc gcggctgaat tttctccggt ccgcgtattg
     2161 ccaactcagc catcattgcc atcgcttgcg gcgcggtggc aaacatctca tcaacaatcg
     2221 cttcgcgttc agcttcacta cgttctggaa agcagagcga cagattgatt aacgcacgac
     2281 ggcgtgagct ttttcccagt cgtccggcaa aacgtcccag ccgtgccaga atgggatcac
     2341 ggaactttgg cggcgttaaa gcgatacccg ccatcgctgc tacacccagc catgctcccc
     2401 agtagcgcgg gtggcgaaag gatttatcaa actcaggaat gtattcgcta ttattttttt
     2461 tcgtttccat gcttttccag tttcggataa ggcaaaaatc aatctggtga tagtatagcg
     2521 gcgcaacttg ccccgcacca aataaaaaaa gccgatactg actgcgtacc ggctgcgaat
     2581 ggatgttaat taatcaaacc gtagctgcgg cacaatctct ttggcctgcg ccaggaattc
     2641 gcgacgatcg gagccggtca gcccttcggt acgcggcagt tttgccgtca gcgggtttac
     2701 ggcctgctgg tttatccata cttcatagtg cagatgcggc ccggttgaac gtccggtatt
     2761 accggaaagc gcgatacggt cgccacgttt caccttctgt cccggtttca ccagaatctt
     2821 gcgcaagtgc atataacgcg tggtgtagct gcgaccatga cgaatagcca cataataacc
     2881 tgctgcgcca ctgcgtttgg caaccaccac ttcaccgtca cccactgaaa gcactggcgt
     2941 accctgcggc atggcgaaat caacacctct gtgtgctgca acgcgaccgg tcaccggatt
     3001 agtacgacgc gggttaaagt tagacgagat acggaactgt ttcgccgtcg ggaatcgcaa
     3061 gaatcctttc gccagaccag taccgttacg atcgtagaat ttgccatctt cagcgcggat
     3121 agcgtaataa tctttacctt ctgaacgcaa acgtacgccc agcagctggc tttgctcacg
     3181 tttaccatca agcatttctc gagacattaa caccgcaaat tcatcgcctt ttttcagttt
     3241 gcggaaatcc atttgccact gcatggcttt aatcactgcg ctcacttcgg cgctggttaa
     3301 accggcgttt ctggcgctgg caacaaagct ccccccgacg gtacctttca gcagattgtt
     3361 aacccactct ccttgctgca tttcgctggt cattttaaaa ccgttagcgg cagtacggtc
     3421 ataggttcgg gtttcacgac gagacacttc ccaggtgagg cgctgcagtt cgccgtccgc
     3481 ggttaatgtc caggagagtt gttgaccgat tttcaggtta cgcaattctt tgtcggccgc
     3541 agccagttgg gtgatatcac ccatatcaat accatactga ttgagaatgc tgcttagcgt
     3601 atcgccagtg gaaacaacat attcatgcac gcccgcttca ccggcgattt tgtcatccag
     3661 ttcgtcctgg ggaatggctt catcttcttg tgcagcttga tcaatcggct cactggcttc
     3721 aggtaagagc gaacgaattt cgttctgttc cagctcaatg gttttgacaa ttggcgtggc
     3781 atcgcggtga taaacatagg gccgccagac agcgacggcc agagtaagaa cggtgagcga
     3841 ccccaacata acgcggtgtg gtcgcggtaa attattaaac gccagggcga cagagcgggc
     3901 tatctgttgc acgtaatcac ttcctcatta atctcctttc aggcagctcg catactggtt
     3961 ggctaattga ctcaggaatt ctgaatagct tgttttaccc agtttgatat tcgtccccag
     4021 gggatccaac gttcccatac gaacggatgt ccctcgtgcg acgctctcaa cgaccgctgg
     4081 cctgaactgt ggctcagcaa aaacgcaggt tgctttttgc tcaaccaact gtgttcttat
     4141 ttcatgtaaa cgctgcgcgc caggttgaat ctcagggtta acggtaaaat gaccaagcgg
     4201 tgtcagtccg aactgttttt cgaaatagcc gtaagcatcg tgaaaaacga aataaccttt
     4261 ccccttgagc ggcgcgagct cgttaccaac ctgcgtttcg gttgaggcta attgtgcctc
     4321 aaaatccttc aggttggcgt caagtttggc tcgactttgc ggcataagtt ccactaattt
     4381 tccatggatt gcaaccgctg tagcccgcgc tatctctggg gaaagccaaa gatgcatgtt
     4441 gaaatcgccg tgatggtgat cttcgtcact tttttccgcg tggtcgtgat catcatcatc
     4501 gccgtgaata cttttcatca gtagcggttt cacatcttca agctgcgcaa tcgttacctg
     4561 cttcgctcct ggtaatttgc ttaccggttt ttgcataaac gcttccatct ccgggccaac
     4621 ccaaacgact aagtccgcgt tctgtaagcg ttttacatcc gatgggcgca gtgaataatc
     4681 atgttctgaa gcgccgtcag gaagtaaaac ctctgtttct gttaccccat cagcaatggc
     4741 agaagcgatg aacccaacgg gtttaagcga agcgacaacg gcggcatctg cggcctgtgt
     4801 tgcacctccc cagagagcgg cggataatgc tgcgaaaaga agcgtttttt tatgtaacat
     4861 aatgcgacca atcatcgtaa tgaatatgag aagtgtgata ttataacatt tcatgactac
     4921 tgcaagacta aaattaacat gacaagtctg gtttccttgg aaaatgtctc ggtttctttt
     4981 ggccaacgcc gcgtcctctc tgatgtgtcg ctggaactta aacctggaaa aattttgact
     5041 ttacttgggc caaatggcgc aggtaagtcg acactggtac gggtagtgct cgggctggta
     5101 acacccgatg aaggggttat caagcgcaac ggaaaactgc gcatcggcta tgtaccgcag
     5161 aagctgtatc tcgacaccac gttgccactg accgtaaacc gttttttacg cttacgccct
     5221 ggtacacata aagaagatat tttgcctgca ctgaaacgtg tccaggccgg gcacctgatt
     5281 aacgcaccga tgcaaaagct ctctggtggc gaaacgcagc gtgtactgtt agcgcgagca
     5341 ttgttaaatc gcccgcaatt attagtgctg gatgaaccca ctcaaggcgt ggatgtaaat
     5401 ggccaggtgg cgttatatga ccttattgac caactgcgtc gtgaactgga ttgtggcgtt
     5461 ttaatggttt ctcacgatct gcatctggtg atggcaaaaa ccgatgaagt gctgtgcctg
     5521 aatcaccaca tttgttgttc cggcacaccg gaagttgttt ccctgcatcc ggagtttatt
     5581 tcaatgtttg gtcctcgtgg tgctgaacaa ctgggtatct atcgccatca tcataaccat
     5641 cgtcacgatt tacagggacg aattgttttg cgtcggggaa atgatcgctc atgattgaat
     5701 tattatttcc cggttggtta gccgggatca tgctcgcctg tgccgcgggt ccgctgggtt
     5761 cgtttgtagt ctggcgtcgt atgtcttatt tcggtgatac gctggctcat gcctcattac
     5821 ttggtgtcgc gtttggtttg ttgctggacg tgaatccatt ctatgcggtg attgccgtta
     5881 cgctgctgct ggcgggcggt ctggtatggc tggagaagcg tccacagctg gcgatcgaca
     5941 cgttattagg gattatggcg cacagtgccc tgtcgctggg cctggtggtc gttagtctga
     6001 tgtctaatat tcgtgttgat ttgatggctt acctgttcgg tgatttgctg gcagtgacgc
     6061 cagaagatct catctctatt gcgattggcg tggtcatcgt ggtggctatt ttgttctggc
     6121 aatggcgcaa tttgctgtcg atgacgatta gcccggatct ggcgtttgtt gatggtgtga
     6181 aattacagcg cgtgaaattg ttgttgatgc tggtgacggc attgacgatt ggtgtagcga
     6241 tgaaatttgt cggtgcgttg attattactt cgttgctgat tattcctgca gctactgcgc
     6301 gtcgctttgc ccgtacgccg gaacagatgg ctggtgtcgc tgttttggtg gggatggtgg
     6361 cagtaactgg cggtttaacc ttttccgcgt tttacgatac gccggcgggt ccgtcggtag
     6421 tgctatgtgc tgcactgtta tttattatca gtatgatgaa aaagcaggcc agctaatctg
     6481 tcgctgaaca catttgtcgg atgcggcgcg agcgccttat ccgacctacg gttcgctatc
     6541 tctggtaggc cggataagac gcgaatagcg tcgcatcagg cacactgcca atgtcggatg
     6601 cggtgcgagc gaccaatccg acttacggca tttctggcgg cgttatgcca aagtgattcc
     6661 acgcccgcgt cgtcgccata cgcccacgcg gtgtacgctg caaaaagcct tgctgaatca
     6721 gataaggctc cagcacatcc tcaatggttt cacgttcttc gccaatggct gccgccaggt
     6781 tatccagacc taccggccca ccaaagaact tatcgattac cgccagcaac aatttgcggt
     6841 ccatataatc gaaaccttca gcatcgacat tcaacatatc cagcgcctga gcagcgatat
     6901 ctgccgagat ggtgccatcg tgcttcactt cggcgaaatc acgcactcga cgcagcagac
     6961 ggttggcaat acgtggcgta ccgcgagcgc gacgagcaac ttccagcgcg ccgtcatcac
     7021 tcatctcaag ccccataaag cgtgcgctgc gactgacgat atattgcaga tccggcacct
     7081 gataaaactc cagacgttgc acaataccaa aacggtcgcg caacggtgat gtcagcgaac
     7141 ctgcgcgcgt ggttgcacca atcagggtaa acggcggcaa atcaatttta atggagcgtg
     7201 ccgccggacc ttcaccaatc atgatatcca gttggtagtc ttccattgcc gggtacagca
     7261 cttcttcaac aactggcgat agacggtgga tctcatcaat aaacagcacg tcatgcggtt
     7321 caaggttagt gagcatcgct gccagatcgc ccgccttttc cagcaccgga ccagaagtcg
     7381 tgcgtaaatt aacgcccatt tcattggcga caatgttggc aagcgtagtt ttacccaacc
     7441 ccggaggacc aaaaatcaac agatgatcga gggcatcgcc gcgcagtttc gctgctttga
     7501 tgaaaatctc catctgtgaa cgaacctgcg gctgaccaac atactcttcc agtaatttag
     7561 ggcgaatggc gcgatctgcc ccatcttccg gcaaagtggt accggcagaa atcagacggt
     7621 ctgcttcaat catcctttac ctcataacgc ggcgcgtagg gcttcgcgaa ttaatgtttc
     7681 actgctggcg tcagtgcgag cgattttgct caccatgcgg cttgcttctt gtggtttata
     7741 gcccagcgcc accagcgcgg caaccgcttc ctgttcagca tcgtcggttg ccgggctggc
     7801 aggcgacgtg agcaccagat cggcggctgg cgtaaagaga tcgccgtgca aacctttaaa
     7861 tcggtctttc atttcgacaa ttaaacgctc ggcggttttt ttaccaatgc ccggcagttt
     7921 caccagtgct cccacttctt cacgttcaac ggcattaacg aactgctgcg ctgacattcc
     7981 ggagaggatc gccagcgcca acttcgggcc gacgccgttg gttttgatta actctttaaa
     8041 caacgtgcgc tcttgtttat tgttaaaacc gtacagcagt tgcgcgtctt cacgcaccac
     8101 aaagtgggtg aaaacgatcg cttcctgacc cgcttcaggg agttcataaa aacaggtcat
     8161 cggcatatgc acttcatagc ctacgccgcc cacttcaatt aacaccagcg ggggttgttt
     8221 ttcaatgatg atgcctctga gtctgcctat cacatgacgc tcctgcgtaa tgaatcaaag
     8281 ataatgctgt atgataaaaa aatgctggat agatatccag cgaaggatga agaaaacttg
     8341 cgaggtgtct cgatgaactg aaaaatggca cagtataatt tattctacag gttatattgg
     8401 aagcaaatat tttaatatta catattcagt gaataaatgc gtaataaaaa tatacattgc
     8461 gcctcctgaa aaaaaataaa ttttttatgc tattacgtat attcgtatct atttcaatgg
     8521 aatgacaacg tgaatattaa ttaccctgct gaatatgaaa ttggagatat cgtctttaca
     8581 tgtataggtg ctgccttatt tggtcaaata tcagctgcat caaattgctg gagtaatcac
     8641 gtcgggatca ttatcggtca taacggtgaa gacttcctgg ttgcagaaag ccgggttccc
     8701 ctttcaacca tcaccacgct atctcgtttt attaaacgtt cgtctaatca acgctatgct
     8761 ataaagcgat tagacgctgg gctgacagaa caacaaaaac aacgaattgt tgaacaggtt
     8821 ccctcccggc tacgcaaact ttaccacacc ggttttaaat acgaatcttc gcgccagttc
     8881 tgttcaaaat ttgtttttga tatttataaa gaagcgctat gcattccggt gggtgaaata
     8941 gaaacgtttg gagaactgtt aaatagcaat ccgaatgcaa aactcacttt ctggaaattc
     9001 tggttcttag gttctattcc gtgggagcgt aaaaccgtca cgccagccag tttgtggcat
     9061 catccgggtc tggtgttgat tcacgcggag ggagttgaaa cgcctcagcc tgaactgacc
     9121 gaggcggtat aacttaacgc agtcgccctc tcgccaggtt cagccgcgat tcgctcatct
     9181 gcattgcatt ctggctgacg tgacaatggg taatcgcgat agctaacgca tcggcggcat
     9241 ccgcctgtgg attagcgggc agtttcagca aggtgcggac catatgctgc acctggcttt
     9301 tttcggcact accaatacct accaccgttt gctttacctg acgtgccgcg tattcaaata
     9361 ccggcaattc ctgattcacc gccgccacaa tcgccacgcc gcgcgcctgc cccagtttca
     9421 gagccgagtc ggcatttttg gccataaaga cttgttcaat ggcgaaataa tctggctgga
     9481 actgggtgat gatttccgtc acgcccgcat agatgagctt cagacgagac ggtaaatcat
     9541 ccactttggt gcgtatgcat ccgctaccca ggtaggacag ttgcctgcct acctggcgga
     9601 tgacaccata gccgatcacg cgcgaacccg ggtcaatgcc gagaataata gccatcacgc
     9661 gtctccgttt tgctgtttag caggccccat cagagagttg ctgcaacctc atcagagatt
     9721 tcaccgttat ggtagacttc ctgcacgtcg tcgcaatctt ccagcatatc gatcagacgc
     9781 atcagtttcg gtgcggtttc tgcatccata tcagctttgg tggacgggat catggaaact
     9841 tccgcgctgt ctgctttcag acctgccgct tccagagcgt cgcgcacttt acccatttct
     9901 tcccatgcag tgtagacgtc aatcgcgccg tcatcatagg tcacaacgtc ttcagcacct
     9961 gcttccagtg ctgcttccat aatggtgtct tcatcgcctt tctcgaagga gatcacgcct
    10021 tttttgctga acagataggc aacggaacca tcagtaccga ggttaccgcc acatttgcta
    10081 aatgcatgac gcacttcagc aacggtacgg ttgcggttgt cagacagaca ttcaatcatg
    10141 attgccgtgc cgccaggacc gtaaccttcg tagatgatgg tttccatgtt tgcatcatca
    10201 tcaccgccca caccacgtgc aattgcgcgg ttcagtgtgt cacgggtcat gttgttagac
    10261 agtgctttat ccaccgccgc acgcagacgc gggttagcgt ccggatcgcc accgcccagc
    10321 ttagccgcgg ttaccagctc acgaatgatt ttagtgaaga ttttaccgcg cttagcatcc
    10381 tgcgcagctt tacgatgtct ggtgttggcc catttactat gacctgccat aaaaatatct
    10441 ccagatagcc ctgcctgttc aggcagcgtt aattacaaac tgttcaatcg cctgccggtt
    10501 gctccaggac ttagtgagcg ccgcggcagc aggcgcatca agccacttgt aagccagatg
    10561 ttcagtgaag actacctggc gttcgtgcgg gagcgcaaga cagaaccatg attccgtatt
    10621 acgcgtcacg cccggcgcat agcgatgacg taaatgtgaa aaaatttcaa actctaccgt
    10681 gcgctgacag tcaattaagg tcagttgttc agcgacaaca tcaatggtga cctcttcctt
    10741 tacttcgcgc atggcagctt gcggcgcggt ttcaccctct tccacgctgc cggttaccga
    10801 ctgccagaaa tcgggatcgt cacgccgctg caacatcagc acccgtttcg tatcttgtgc
    10861 atagatgacc actaagatcg aaacgggacg cttatacact ttatccttca cgctgcctct
    10921 tcgttgactg ccttcgctca tcccattcac atagttatct atgctcatgg gagttcactc
    10981 agttgccgcc ttgatgcaac gcgaatgatt tcgtgtattt gagtcatatc agttattctc
    11041 agccttcttc acaacctgaa tgctcagctc agccagtaca gtcgggttag caaagctcgg
    11101 tgcttcagtc atcagacacg ctgccgccgt ggttttcggg aaggcgataa cgtcacggat
    11161 attgtcggtg ccagtcagca gcatggtcag acggtcaaga ccgaatgcca gacctgcgtg
    11221 cggcggagta ccgtatttca gggcgtcgag caggaagccg aatttctcgc gctgttcctc
    11281 ttcgttgata cccagaatac caaacaccgt ctgctgcata tcaccattat ggatacgtac
    11341 tgaaccaccg cccacttcgt aaccattgat gaccatatcg taagcgttcg ccaccgcatt
    11401 ttccggtgca gctttcagtt ctgcagcagt catgtctttc ggtgaggtga acggatggtg
    11461 cattgccgtc aggccgcctt caccgtcgtc ttcaaacatc gggaagtcga taacccacag
    11521 cggtgcccat ttgctttcgt cggtcagacc aaggtcttta cccactttca ggcgcagtgc
    11581 gcccatcgcg tcggcaacaa ttttcttgtt gtcggcaccg aagaaaatca tatcgccatc
    11641 ttgcgcggca gtacgatcca ggatggcttc gatgatttct gcattaagga acttcgctac
    11701 cgggctgttg ataccttcca gacctttcgc gcgttcgtta actttgatgt aagccagacc
    11761 tttcgcgccg tagattttaa cgaagttacc gtattcgtcg atctgcttac gggtcagcga
    11821 tgcgccaccc ggaacacgta gagcggcaac acggcctttc ggatcgttcg ccggccccgc
    11881 aaatactgca aactcaacag atttcagcag atcggcaacg tcggtcagtt ccatcgggtt
    11941 acgcagatcc ggtttatcag aaccataacg gcgttctgct tccgcaaagg tcattaccgg
    12001 gaaatcgccc agatccacgc ccttcacttc cagccacaaa tgacgcacca gcgcttccat
    12061 cacttcacgc acttgcggcg cggtcatgaa agaagtttcc acatcgatct gagtaaattc
    12121 aggctgacga tcagcgcgca ggtcttcgtc acggaagcat ttaacgatct gatagtaacg
    12181 gtcaaaaccg gacatcatca gcagctgttt gaacaactgt ggggattgcg gcagtgcgta
    12241 gaatttacct ttgtgcacac gagaaggcac caagtagtca cgcgcgcctt ccggcgtggc
    12301 tttggtcagc atcggagttt cgatgtcgag gaagccgtgg tcatccataa aacggcgcac
    12361 caggctggtg attttagcgc gggttttcag gcgctgcgcc atttccgggc gacgcaggtc
    12421 gaggtagcgg tatttcagac gcgcttcttc ggtgttgacg tggttagagt caagcggcag
    12481 aacatctgcg cggttgatga tagtcagcga ggacgccagc acttcaattt cgccggtcgc
    12541 catatcgcgg ttaatatttt tttcgtcacg cgcacgtacg gtgcccgtaa cctgaatgca
    12601 gaactcatta cgcagttcag aggccagctt taacgcgtcc gcacgatccg gatcgaaaaa
    12661 tacctgcacg ataccttcgc ggtcgcgcat atcgatgaag atcaggctac caagatcacg
    12721 acgacggttg acccaaccac acagagtcac ctgctgcccc acgtgggaca aacggagctg
    12781 tccacaatat tctgtacgca tgagatatcc cttaacttag ctgccggcgg atgccccctg
    12841 ctgcgcaggt gaccaagtcg cagcgttagc tgtatgtcac aactgaatga aaaaaggcgg
    12901 ctattatact ggaaattctg ccgcaccgta agagcctggc ccgcgctgta acgcctcgtt
    12961 accactttat atcgggcctg aaatcagact ctacgccagt ttgctataaa ggtgttgccc
    13021 gaactcataa aaattaacaa aatttgtcgt tccgccatcg gctaatcgca ttaaggtgtg
    13081 aggcacgatt ttgttttttc aggagtcagc atgcttgaac ttaatgctaa aaccactgcg
    13141 ttggtggtga tcgatttaca agaaggcatc ttaccttttg ccggtggtcc acatactgcc
    13201 gatgaggtag ttaatcgcgc cgggaagctg gcggcgaaat ttcgcgccag cggtcagccc
    13261 gtgtttctgg tgcgagttgg ctggtctgcc gattacgccg aagcattaaa acagccggtt
    13321 gatgccccct cccccgctaa agtgttgccc gaaaactggt ggcaacatcc agctgcatta
    13381 ggtgcaactg acagtgatat cgaaatcatc aaacgtcagt ggggtgcgtt ttacggtacg
    13441 gatctggagt tgcaactgcg ccgccgaggt atcgatacaa tagtgttatg tgggatctcg
    13501 accaatatcg gtgttgaatc caccgcccgc aatgcctggg aacttggctt taatctggtg
    13561 attgccgaag acgcctgtag tgccgccagc gccgagcaac acaataacag cattaatcat
    13621 atctacccgc gcatcgcccg tgtgcgtagc gtggaagaga tccttcacgc gttatgattt
    13681 acatcggtct accgcaatgg tcgcatccta aatgggtgcg gttggggatc accagccttg
    13741 aagagtatgc ccgccacttt aactgcgtga tacgggattt ttaaaaatca ctaaagaacg
    13801 cccaagagca tgtgttttct ttagaataat caaagcgttg gattgccatg ccatatgaaa
    13861 atcggcaaag ttcatatgta acaaagtgtc ccaatcatgc cccaaaatgc cccacaacaa
    13921 atatttttgc cccatgcatg ccccaaaaaa tcactttccc tcactcttga cactgtttat
    13981 ccatacagtt aaaaataata ctgtataaaa acacagtgta gagggatttt tatgcgtatt
    14041 gaaatctgca tagccaaaga gaaaatgact aaaatgccaa ccggtgctgt ggatgcgtta
    14101 aaggaagaat taacccgacg catcagtaaa cgttatgacg atgtagaggt gatcgtaaaa
    14161 gccaccagca acgatggcct ttctgttacg cgcaccgccg ataaagattc agctaaaact
    14221 tttgttcagg aaactctgaa agatacctgg gagtctgctg acgagtggtt tgttcactaa
    14281 ttagcacgta aaatcagtaa cggctggaaa tcattcaata ctcgcactat cggaagttca
    14341 ccagccagcc gcagcacgtt cttgcatacg acgtgtctgc ggtctcaact ccgcccgccc
    14401 ccgccaatca tgattgggca gtaccagtac agcactagca aagacaggaa attagcagtc
    14461 tcagcaaaac accgaccaga cggtgagaag acaaaaagat acgcaaagat gccgcggttc
    14521 cagaagaata aaaaaccgct aatggacagc ctccttaaca cagtttttta tatacatgat
    14581 tcactgatac cagtggcatt aatagctatt atatagcttc agctcctttt ttcatacctt
    14641 aaggaaaaat tgccccctcc cccaaaaagt atcgtttttt tacattaaac cgatccagtt
    14701 taggatagtg taaagcagaa gttcttcaat atataacata tggagttaat atgcttacac
    14761 aaactatata ttcgcctcct ccagaagttc ctgtctactg ctgaagggaa ccaataacat
    14821 tgcaaagcag ataaaaaaga atccaacacc tctcaacata aagaatacgc cctgatattt
    14881 atgctcccga caaataacaa tcacagatta atttcaaatt cgttctccac ttattcaatc
    14941 gacactagcc gtgcatatga aaattatcta acccattgga ctgaatggaa aaataaccgc
    15001 atacaagaag aacaacgaga catcgctttt cagcgactag tatcatgtct acaaaaccaa
    15061 gagacgaacc tggacttgtc tgaattaggc ctgacaacat tacctgaaat ccccccggaa
    15121 attaaatcaa ttaatataag taaaaataat ttaagcttaa tctccccatt gcctgcgtcc
    15181 cttacacagc ttaatgtcag ctataacaga cttattgaac tgcctgcttt gcctcaagga
    15241 cttaaattat tgaatgcgtc ccacaatcaa ctaatcacac tacccacact ccccatatct
    15301 ttgaaggagc ttcatgtctc aaataatcaa ttatgttctc ttcctgtttt accagaacta
    15361 ctggaaacat tagatgtatc atgtaatggg ctggcagttt taccaccttt accattttct
    15421 ttacaagaga ttagcgcaat agggaatctt cttagtgaac tcccccctct acctcacaac
    15481 attcactcca tatgggcaat cgacaatatg ttaaccgata ttccatacct gccggaaaat
    15541 ttaaggaacg gttattttga cataaatcag ataagtcata tcccggaaag cattcttaat
    15601 ctgaggaatg aatgttcaat agatattagt gataacccat tgtcatccca tg
//
LOCUS       DAZPAC010000086        15582 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZPAC010000086 DAZPAC010000000
VERSION     DAZPAC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.086"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..2065
                     /locus_tag="ACHUPM_002283"
     CDS             <1..2065
                     /locus_tag="ACHUPM_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR0870045.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2081..3115
                     /locus_tag="ACHUPM_002284"
     CDS             2081..3115
                     /locus_tag="ACHUPM_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFR0870046.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3197..3535)
                     /gene="rcnB"
                     /locus_tag="ACHUPM_002285"
     CDS             complement(3197..3535)
                     /gene="rcnB"
                     /locus_tag="ACHUPM_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR0870047.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3754..4578)
                     /gene="rcnA"
                     /locus_tag="ACHUPM_002286"
     CDS             complement(3754..4578)
                     /gene="rcnA"
                     /locus_tag="ACHUPM_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFR0870048.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4699..4971
                     /gene="rcnR"
                     /locus_tag="ACHUPM_002287"
                     /pseudo
     CDS             4699..4971
                     /gene="rcnR"
                     /locus_tag="ACHUPM_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5063..5159
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5194..5982
                     /gene="thiM"
                     /locus_tag="ACHUPM_002288"
     CDS             5194..5982
                     /gene="thiM"
                     /locus_tag="ACHUPM_002288"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR0870049.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5979..6779
                     /gene="thiD"
                     /locus_tag="ACHUPM_002289"
     CDS             5979..6779
                     /gene="thiD"
                     /locus_tag="ACHUPM_002289"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR0870050.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6844..7662
                     /locus_tag="ACHUPM_002290"
     CDS             6844..7662
                     /locus_tag="ACHUPM_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFR0870051.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7714..8460
                     /locus_tag="ACHUPM_002291"
     CDS             7714..8460
                     /locus_tag="ACHUPM_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0870052.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8434..9399)
                     /locus_tag="ACHUPM_002292"
     CDS             complement(8434..9399)
                     /locus_tag="ACHUPM_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR0870053.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9396..10400)
                     /locus_tag="ACHUPM_002293"
     CDS             complement(9396..10400)
                     /locus_tag="ACHUPM_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFR0870054.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10397..11674)
                     /locus_tag="ACHUPM_002294"
     CDS             complement(10397..11674)
                     /locus_tag="ACHUPM_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFR0870055.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11931..12983
                     /gene="fbaB"
                     /locus_tag="ACHUPM_002295"
     CDS             11931..12983
                     /gene="fbaB"
                     /locus_tag="ACHUPM_002295"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR0870056.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13302..14156
                     /gene="gatY"
                     /locus_tag="ACHUPM_002296"
     CDS             13302..14156
                     /gene="gatY"
                     /locus_tag="ACHUPM_002296"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR0870057.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14185..15442
                     /gene="gatZ"
                     /locus_tag="ACHUPM_002297"
                     /pseudo
     CDS             14185..15442
                     /gene="gatZ"
                     /locus_tag="ACHUPM_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15452..>15582
                     /locus_tag="ACHUPM_002298"
     CDS             15452..>15582
                     /locus_tag="ACHUPM_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR0870058.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         4088 a         3728 c         3987 g         3779 t
ORIGIN      
        1 tggctatgtt ccaccggaaa actgggagcg gggtattaat gcgttttata cctcttatta
       61 tgtgagtcag tattacagtg actataaagc gtcgggtaat agtaagagta catatgtacg
      121 ttttaacagc gggttaaatt tactggggtg gcaactgcat tctgatgcca gtttcagtaa
      181 aacaaataac aatccagggg tgtggaaaag caataccctg tatctggaac gtggatttgc
      241 ccaacttctc ggcacgcttc gcgtgggtga tatgtacaca tcaagcgata tttttgattc
      301 tgttcgcttc agaggtgtgc ggttgtttcg tgatatgcag atgttgccta actcgaaaca
      361 aaattttacg ccacgggtgc aggggattgc tcagagtaac gcgctggtaa ctattgaaca
      421 gaatggtttt gtggtttatc agaaagaggt tcctcctggc ccgtttgcga ttacagattt
      481 gcagttggcc ggtggtggag cagatcttga tgtcagcgtg aaagaggcgg acggttcggt
      541 aaccacctat ctggtgcctt atgcagcggt gccaaatatg ctgcaacccg gcgtgtcgaa
      601 atatgatttt gcggcgggtc gtagccatat tgaaggggcg agcaaacata gtgattttgt
      661 ccaggcgggt tatcagtatg gttttaataa cttattgacg ttgtatggtg gctcgatggt
      721 tgcgaataat tattacgcgt tcactttggg gactggctgg aatacacgca ttggtgccat
      781 ttccgtcgat gccacgaagt cgcatagtaa acaagacaac ggcgatgtgt ttgacgggca
      841 aagttatcaa attgcctaca acaaatttgt gagccaaacg tcgacgcgtt ttggtctggc
      901 ggcctggcgt tattcgtcgc gtgattaccg gacatttaac gatcacgttt gggcaaacaa
      961 taaagataat tatcgccgtg atgaaaacga tgtctatgac attgccgatt attaccagaa
     1021 tgattttggt cgcaaaaata gcttttccgc caatatgagc cagtcattgc cagaaggttg
     1081 ggggtctgtg tcattaagta cgttatggcg agattactgg gggcgtagcg gcagtagtaa
     1141 ggattatcag ttgagttatt ccaacaacct gcgacggata agctataccc tcgcggcaag
     1201 ccaggcttat gacgagaatc atcatgaaga gaaacgtttt aatattttta tatcgattcc
     1261 ctttgattgg ggtgatgacg tttcgacgcc tcgtcggcaa atatatatgt ctaactcaac
     1321 gacgtttgat gatcaggggt ttgcctcaaa taatacggga ttatcaggaa cagtagggag
     1381 tcgggatcag ttcaattatg gtgtcaacct gagtcatcaa catcagggaa atgaaacgac
     1441 agctggagcg aatttaacct ggaacgcgcc ggtggcaaca gtgaatggca gttatagtca
     1501 gtcgagtact tatcgacagg ctggagccag tgtttcaggg ggcattgtcg cctggtcggg
     1561 tggcgttaat ctggcgaacc gtctttccga aacgtttgct gtgatgaatg cgccaggaat
     1621 taaagatgct tatgtcaatg ggcaaaaata tcgcacaaca aaccgtaatg gagtggtggt
     1681 atacgacgga atgacacctt atcgggaaaa tcacctgatg ctggatgtgt cgcaaagcga
     1741 tagcgaagca gaattacgtg gcaaccggaa aattgccgca ccttatcgcg gcgcggttgt
     1801 actggttaat tttgataccg atcagcgcaa gccctggttt ataaaagcgt taagagcgga
     1861 tgggcaacca ttaacgtttg gttatgaagt caatgatatc cacggtcata atattggcgt
     1921 tgtcggccag ggaagccagt tatttattcg caccaatgaa ataccgccat cggttaatgt
     1981 agcaattgat aagcaacaag gactttcatg cacaatcacc ttcggtaaag agattgatga
     2041 aagtagaaat tatatttgcc agtaatagtc aggtggttct atggaaattc gcataatgct
     2101 atttatatta atgatgatgg ttatgcctgt gagctatgcg gcatgttata gtgagttatc
     2161 tgttcagcac aatttggttg ttcaggggga ttttgcactt actcaaacac aaatagcgac
     2221 atatgagcat aattttaatg attcgtcatg cgtgagtaca aatactatca cccctatgag
     2281 ttcgtcggat attattgttg ggctttataa cgataccata aaattaaatt tacattttga
     2341 atggaccaat aaaaacaaca tcacgttgtc aaataatcag accagtttca ccagtggtta
     2401 ttcagttacg gtgacacctg cggccagtaa tgcaaaagtg aatgtttctg cggggggcgg
     2461 cggttcagtg atgattaatg gtgttgcgac attatccagt gcttcatcat cgacacgcgg
     2521 gagtgccgca gtacaatttc tactgtgttt attaggtggc aagtcatggg atgcatgtgt
     2581 aaatagctac agaaatgcat tggcacaaaa tgcaggtgtc tattccttta atctgacatt
     2641 gtcatacaac ccgataacca caacctgcaa accggacgat ttattaatta ctttagacag
     2701 tattcccgtt tcacaattac cagccacagg taacaaagca acaataaata gtaaacaagg
     2761 ggatattatt ctgcgttgta aaaatttatt aggtcaacaa aatcaaacat cacggaaaat
     2821 gcaggtgtat ttatcaagtt ctgatttgtt agccggcagt aacacgatcc tgaaaggtgc
     2881 agaaaataat ggagtcggat ttatgcttga aagcaatggt tctccagtca catttttaaa
     2941 tatcactgat aacagcaaag gatacacaaa tttaaaagaa attgcggcaa agtcaaaact
     3001 tacagataca acggtttcaa ttccgataac tgccagttac tacgtctacg atacaaacaa
     3061 agttaaatct ggcgcactgg aggcaaccgc attaatcaac gtgaaatacg actaataaca
     3121 agcaagacga gcaagtggct aataataaaa aataacaagg tgtctgcatt tccccctcat
     3181 gatgaggggg ctttttttag cgatgataaa aaatctcacc gtcgtaggct ttaatgattt
     3241 taccgtcggt gtcgctgatc aacacgtacg cgccacccat ataggtccag tgcgtcccgg
     3301 catcaggcgc gggcaggtta cgtaactgcc actgcttaat gttgtactca tcggtgcggt
     3361 acatctctgg gacggtatca cctggtttga aatgcttaaa gtcagcaatg aaactactta
     3421 actcatactt agaaattccc gaggtattgg tcgaacccgc ggtggctggt gcggcccagg
     3481 cggcactggt aaccagcaaa agcacaccca gcaacatctt atttttaatg gtcataaact
     3541 ctccactttg gggatctgta atatcagtcg catatctttc ctgccataca atataactga
     3601 caagcgatat tttgttagtg atcccatgat ttcgtaaaaa gtgtaaatga aaggatgccc
     3661 taaaagcatc cttagcgagc agagagtcag cggaaatagc tgatttaaga atcatcctgg
     3721 gctgaagata acatgcaagc ctaaaggatt gagttatcgc attatgccca tgaagccatg
     3781 tacgcccata tacacaccga ctaagccaat caacagactg gaaaaatagg gggcgcgttt
     3841 agcgagagtg ttaaatccgc tccagcgttt tgcgacctgc tgaacgctga ttgctgcgcc
     3901 aacgcctacg gtgacaagcg ttaacgccag gccaatgctg aaactgacga ccagtgttgc
     3961 gcccagtgtc agggctttca actgaatgca tatcaacagc acggtaattg ctgccgggca
     4021 ggggataagg ccaccggtta agccaaatag caaaatttgc cagttggtga cctctctacc
     4081 atcaaagcgt cgtttaatgt cattggcatg ggctcgtgca tgggcatcct gatactcgcc
     4141 atgttcgtga tggtgatgat gtccatggtc gtggtgatct tcgtgatcgt gatgatgatg
     4201 ctcataatca tactcgtgca tattctccag ccagttgcgt tcgccgcgcc aggtacgcca
     4261 gaacatccag aacgcggtgc caataatgat cactgcggaa atcagctgga gccacggttc
     4321 tgctgattga gcagtaaagc gcttgctgat caccatcccg ccaaaggcaa ttaaccagac
     4381 cactgcggta tgcgaaatag ttgctgccag tccgagcatc accgcttgtt taatggtgcc
     4441 tttgatggcg atgataaacg ccgccatcat cgtttttgag tgccctggtt ccaggccatg
     4501 aagcgcacca agtaagatgg cgctggggat gaagaaccag gcgtttcctt gctgaagaag
     4561 agttgtaaat tcggtcatga taataattct tagtattaat tcggcaatct gattctactc
     4621 cccccagtac ctgatactac cccccagtag attaatagtg ctatgatatt ttcatattct
     4681 tgttaaccag gtgttgccat gtctcataca atccgtgata aacagaaact gaaagcgcgt
     4741 gccagtaaga ttcagggcta ggtcgtggcg ctcaagaaaa tgctcgacga gccgcatgaa
     4801 tgcgctgcag ttttacaaca gattactgct atccgtggcg cggtaaacgg tctgatgcgg
     4861 gaagtgatta aaggtcatct gacggaacac atcgttcacc agggggatga gctaaaacgt
     4921 gaagaagatc tggatgtcgt tctgaaggtg ctggattcat atatcaaata atttattaac
     4981 gcgattgtaa aactgccgtt tttcctcgtt tacaacgcgt gcgctggaca ttaccatcct
     5041 cctctgcgat ttatcatcgc aaccaaacga ctcggggtgc ccttctgcgt gaaggctgag
     5101 aaatacccgt atcacctgat ctggataatg ccagcgtagg gaagtcacgg accaccaggt
     5161 cattgcttct tcacgttatg gcaggagcaa actatgcaag tcgacctgct gggttcagcg
     5221 caatctgcgc acgcgttaca cctttttcac caacattccc ctcttgtgca ctgcatgacc
     5281 aatgatgtgg tgcaaacctt taccgctaat accttgctgg cgctcggtgc atcgccagcg
     5341 atggttatcg aaaccgaaga ggccagtcag tttgcggcta tcgccagtgc cttgttgatt
     5401 aacgttggca cactgacgca gccacgcgct caggcgatgc gtgctgccgt tgagcaagca
     5461 aaaagctctc aaacaccctg gacgcttgat ccagtagcgg ttggtgcgct cgattatcgc
     5521 cgccattttt gtcatgaact tttatctttt aaaccggcag cgatacgtgg taatgcttcg
     5581 gaaatcatgg cattagctgg cattgctaat ggtggacggg gagtggatac cactgacgcc
     5641 gcagctaacg cgatacccgc tgcacaaaca ctggcacggg aaactggcgc aatcgtcgtg
     5701 gtcactggcg aggtggatta tgttaccgat ggccatcgcg ccgttggcat tcacggtggc
     5761 gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt tgtcgctgcc
     5821 tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat ctgcctgtca ctggatgaaa
     5881 caagccggag agcgcgcagt cgccagaagc gaggggctag gcagttttgt tccacatttc
     5941 cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt aacgctctga
     6001 cgattgccgg tactgatccg agtggtggtg cggggattca ggccgatctt aaaaccttct
     6061 cggcacttgg cgcttatggt tgctcagtta ttactgcact ggtggcgcaa aatacccgtg
     6121 gcgtacagtc ggtgtatcgc attgagcctg attttgtcgc cgcccagctc gattcggtgt
     6181 tcagcgatgt gcgaatcgat accactaaaa tcggtatgtt ggcggaaacc gatattgttg
     6241 aagcggtggc agaacggttg caacgttatc agatccaaaa cgtggtactc gacaccgtta
     6301 tgctggcaaa aagcggcgac ccactgcttt caccttcggc agttgccacg ctacgcagtc
     6361 gattattgcc acaggtttca ttaataacgc caaacttgcc cgaagcagcc gccttgctcg
     6421 acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg ctgttggcga
     6481 tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa agcccggact
     6541 ggctgtttac ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg accaaaaaca
     6601 cccacggtac tggttgtaca ctctctgcgg cgttggctgc actacgcccg cgccatacaa
     6661 actgggctga caccgtacag gaggcaaaaa gctggctttc atcggcgtta gcccaggccg
     6721 acacgctgga agttggtcac ggtattggtc cggttcacca cttccacgcc tggtggtgag
     6781 caccgtacta tagctgcttt ggttattgtc agcctttggc actgacattc agggatgaaa
     6841 ccaatgcagc taagaattac cagtcgaaaa aaactcaccg ctctattatg cgctcttggg
     6901 ctaatttcca tcgttgcaat ttatccgcgt cagacggtga attttttcta ttcgacagca
     6961 gttcagatta cagactacat ccacttctac ggttatcgcc cggttaaatc tttcgctatt
     7021 cgtattcctg ccagttacac cattcacgga atagatgttt cgcgctggca ggagcggatc
     7081 gactggcagc gtgtggcaaa aatgcgcgat aacggtattc gcttacaatt tgcctttatt
     7141 aaggcgacgg aaggcgaaaa gctggtggac ccctattttt cgcgtaactg gcaactaagc
     7201 cgcgaaaatg gcttgctgcg cggggcatat cattatttct ccccgtcggt agcagcaccg
     7261 gttcaggtgc gattatttct gcaaacggta gatttctcac aaggcgattt ccctgcggta
     7321 ctggacgtag aagagcgagg aaaattatcg gcaaaagaat tacgcaagag ggtaagtcag
     7381 tggctaaaaa tggtcgaaaa aagtacgggg aaaaagccga ttatttactc aggagccgtt
     7441 ttttatcaca cgaatctggc gggctatttc aatgaatatc cgtggtgggt ggctcactac
     7501 tatcaacgtc gtccggacaa tgacggcatg gcctggcgct tctggcagca ttccgaccgt
     7561 ggacaggtag atggcatcaa tggtccggtg gattttaatg tgtttaatgg cacggtggag
     7621 gagttgcagg gattcgttga tgggattaaa gaaacgcctt aaataagaag cagtgatata
     7681 ctcccaccag actccctggc aggaaaaaga caaatggaac aagcgcatac ccagctgatt
     7741 gcccaactga acgaacgtat tttagcggcg gataacacgc cgctttatat taagtttgcc
     7801 gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc atggcaatat tttgcccggt
     7861 gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa ggcgatgcag
     7921 gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca gatcaacaac
     7981 atcttcgaat actcgttgaa agaagcgcga ggtttttctc agcaggtggt gttgcgcggg
     8041 aaaaagcccg atacgctatg ggttaacaag cgtgtcgtga aatgtcccga agaagtcgcg
     8101 cagcagctgg cggtcgaagc aggaagtgat gtctttttgc ttaagcgtat tcgctatgtc
     8161 gatgaagaag cggtatcgat tgaggaatcg tgggttccgg cgcatttaat tcatgatgtt
     8221 gatgccatcg ggatttcact ttatgactat ttccgcagcc agcacattta cccacaacgt
     8281 acgcgttccc gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca tattcagtta
     8341 gatagcaaaa taccggtgct ggtgatcaag caagtggcgc ttgaccaaca gcaacggcct
     8401 attgagtaca gcattagcca ctgtcgcagc gatctatacg tttttgtgtg cgaggagtag
     8461 ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg acaacccacg acgccactgc
     8521 attgcccagt aatacggcat ccgccagtgg cagaccagag gccagcccgg caagtacgcc
     8581 acctgcatga ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa atgccggaat
     8641 gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac gaacgatcaa
     8701 cggcgcggca aatttctcct gccattgctc gccaagtgtt gttatctctg cggataaagc
     8761 aaaacgttcg gcggcaatct cagcctcttg acgattgagc gacactaaag gtcgacaggc
     8821 catgatccgc gccagtaatg catccgggat atcgccaata cgtgggccaa aatcgataaa
     8881 cggcgtcacg tcttgcagct cttccagcca ttccactaac aattcgccgc agggcgaggc
     8941 cagttgataa ccggaaaaat agagcaggct gccaggcgca acggttaatc gcgccagcca
     9001 ctggcgattc cactgatttt ccacaccgct gaatgacata aaagtgcgtt cgccatccgg
     9061 ctcaaccagc gccagacacc agccgttatc accttcggcg ttatcgatca ggctgattaa
     9121 gccctctttt gccatccggt tgcgaataat ctccgcccac acgccctgac cgagcggcaa
     9181 ggcattaccc gcttcgatgc cgaggcgctt taacgccacg gcaatattca gtgcgcagcc
     9241 gccaacgtta acgctctgct gtttcagttc gatatcgcac ccacgccagg ggagggcata
     9301 agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc atcaccggct gacgcgtggt
     9361 taattcaggc agcagcgtgt gtaatcgagc gccgctcata ccgcctcccg ttgttgacga
     9421 tatttcgcca gcgctgtggc atagcggttg aaatcaagct gatttaccgc atccagttcc
     9481 gcctttaacg caggatcgat agcgttaacg ccatgcaacg cgccgcaaat tgccgtcgcc
     9541 atagcaccga tggtgtctgt gtcgccgcca aggttagcgc acaggacggc gcagcgattc
     9601 gggtcggttt gtgctagttc aaccagcgca atggcgcacg gaacggactc aatagtgctg
     9661 gtacctgcgc caacgacctg gtaaagctgt tcgctggcgg attcggtgcc gtcggcattg
     9721 cgcacaattt tcagcgcaat ctccagccgc gccgccagtg aggcgctgaa cgtagtgatg
     9781 cgtttttgct gtgcatgtcg cgcaattgaa ggcagggaat caacaatcgc tgaccagctt
     9841 tctccgtcaa tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc aaccgccaga
     9901 tcggatttat gggtcgggct ggacgccagc gctacatcat caataaagga atcaacatca
     9961 cgcgccggaa gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt ggtcacgccg
    10021 ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc aatcttcgag
    10081 gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca gcgcccagtc gagaatatta
    10141 cgcccaatca gatccggatc gatcttgcct tcacgttcca gtaacgcatc cgccagacac
    10201 agcgccatcg aggtatcgtc ggtgaattcg gcgcggttaa aataacaggc cgcgttattc
    10261 tcctttggtc caggaagaaa acggtcaatc cagccaaagt gtgctttaac gcggctgcgt
    10321 ggccaaagct cggagggcat ccccatcgca tcccctaacg cctgcccata aagagcaccg
    10381 agaatacgtt ctgttttcat ttaacttccc cttgtgtcaa cgcaatatcg cgatcatcga
    10441 ccttgatagc cgtaatttcg ttgtcggatt cgcggaaaaa aatcatgaac agcacggcga
    10501 taatggcaat catcaccgcg ccgaaagtcc acatcccgga ccagttgaaa gtcagtccgt
    10561 ttaccggttc ctgataagcg aacatctttt ccatcatcac accgccaaga cgatagccga
    10621 gcaaactgcc gaagccctgg cagcagagcg tgatcagccc ctgcgcagcg gtacgcatat
    10681 gcacgggggc ttttttatcg acatagatgt aagcggtaac gtagtaaaaa tcgtaactta
    10741 cgccgtgaag caaaataccg aggaacagta acgcgtaggt gaaatattca tccgtactac
    10801 cgtaaataaa gaagccatag cggatcgcag cggtgaccag accaagcaat aatacctttt
    10861 tgataccaaa gcgtttagtg aaaaacggca atgccagcat aaagaagatt tcagagaact
    10921 ggccgagcgt catccagccg gtggcgtttt tcatgccaac ttcggtcaga taaccgttgg
    10981 caaagatgta atagaacgcc agtggcatcg caaacaggaa tgaacagaag aaaaagacga
    11041 ggaagttttt atcgcgcagc aggatcagcg catccaggcc gagcatgact ttaatatcca
    11101 ttttgccggt gctttttggt ggcgtgtcgg gcaggaaaaa cgcaaacaca ccgagcagag
    11161 cagaacttcc ggcggtaatc agcagcggga tgttagtcgg tgagatatcg gcatacccca
    11221 gtatttgcgg caagaaacca catgccagac cggaggcaat ccagccgata gtgcccatca
    11281 cacgaatgcg cgggaaatca cgctccacat ccggcacgtt ggcaaaagcg atgctgttag
    11341 tcagcgcaat ggtcggcata taggttagag agtaagccag cagtaacgga aagaacccgg
    11401 caaaagtggt ctgttgcgca gcgaaataca tcagcagcgc gcctgcgaac atcaataccg
    11461 ccagcacttt ttgcgccgag aaaaagcggt cagtgatgga gccaaccaga attggcgaca
    11521 ggatcgccgc aatggcggta caggcatacg accagccaat ttctccggca ctaaaaccgc
    11581 ttttacttaa ccacaaccac aatggcacaa accacgcgcc ccagataaac cattcaacaa
    11641 acatcatgaa cgacagcttt gctgttgttt tcatctttta attccttcat gacaggtaat
    11701 gtaaggtacg cgatgacaat accacttaac aatacctttt aaataccttt gcggtgaaaa
    11761 cttgaccgcg ctaacatttt ttctgatgaa tcgagccaac agaaaacgct gaaaaaacat
    11821 ccaaaagatg gaaaaactcg cccgtaacga ccaggattat gattgcccgc aaaaggcggg
    11881 ccaggacaat ccatagccga tatccaatcg gaatttacgg gagcatagta atgacagata
    11941 ttgcgcagtt gcttggcaaa gacgccgaca accttttaca gcaccgttgt atgacaattc
    12001 cttctgacca gctttatctc cccggacatg actacgtaga ccgcgtaatg attgacaata
    12061 atcgcccgcc agcggtgtta cgtaatatgc agacgttgta caacaccggg cgtctggctg
    12121 gcacaggata tctttctatt ctgccggttg accagggcgt tgagcactct gccggagctt
    12181 catttgctgc taacccgctc tactttgacc cgaaaaacat tgttgaactg gcgatcgaag
    12241 cgggctgtaa ctgtgtggcg tcaacttacg gcgtgttggc gtcggtatcg cggcgctatg
    12301 cgcatcgcat tccatttctc gtcaaactta atcacaacga gacgctaagt tacccgaata
    12361 cctacgatca aacgctgtat gccagcgtgg agcaggcctt caacatgggc gcggtggcgg
    12421 ttggtacgac tatctatttt ggctcggaag agtcacgtcg ccagattgaa gaaatttctg
    12481 cggcctttga acgcgcgcac gagctgggca tggtgaccgt gctgtgggcc tatttgcgaa
    12541 actccgcctt taagaaagat ggcgttgatt accatgtttc cgccgacctg accggtcagg
    12601 caaaccatct ggcggcaacc atcggtgcag atatcgtcaa acaaaaaatg gcggaaaata
    12661 acggcggcta taaagcaatt aattacggtt acaccgacga tcgtgtttat agcaaattga
    12721 ccagcgaaaa cccgattgat ctggtgcgtt atcagttagc taactgctat atgggccggg
    12781 ctgggttgat aaactccggc ggtgctgcgg gcggtgaaac tgacctcagt gatgcagtgc
    12841 gtactgcggt tatcaacaaa cgcgctggcg gaatggggct gattcttgga cgtaaagcgt
    12901 tcaagaaatc gatggctgac ggcgtgaaac tgattaacgc cgtgcaggac gtttatctcg
    12961 atagcaaaat tactattgcc tgatacgcct catacgaaca gcaccacccc gtaggacgga
    13021 taaggcgttt acactgcatc cggcaaccta tgcctgatgc gacgcggaag cgtcttatca
    13081 tgcctacata gcactgccac gtatgtcgga aaaggcgttt acaccgcatc cggcataaaa
    13141 acacgcgcac tttgctacgg cttccctatc gggaggccgt ttttttgcct ttcactcctc
    13201 gaacagtttt catattgtcg ttttgtgatc gttatctcga catttaaaaa caaatcattt
    13261 cattatattt tgaaatcgaa aacaagcaac aggatatgaa aatgtacgtg gtatcgacaa
    13321 agcagatgct gaacaacgca cagcgcggcg gttatgcggt tccggcattc aatattcaca
    13381 atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg ccggtcatca
    13441 tcgccggaac gcctggcaca ttcacttatg ctggtacaga aaatctgttg gcgctggtca
    13501 gcgcgatggc gaagcaatat caccatccac tggcaattca tctcgaccat cacacgaaat
    13561 ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt gacgcctcgc
    13621 atttgccttt tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt tgccatcgct
    13681 ttgatgtcag cgtcgaagcg gagctggggc aacttggcgg ccaggaagat gatgtgcaag
    13741 tcaatgaagc cgatgttttt tataccaacc ccgctcaggc gcgtgaattt gccgaggcaa
    13801 ccggaattga ttccctggcg gtcgccatcg gcacggctca tgggatgtat gccagcgcac
    13861 cggcgcttga tttttctcga ctggagaaca ttcgccagtg ggtgaactta ccgctggtgc
    13921 tgcatggcgc gtcagggtta tcgactaagg atattcagca aaccatcaaa ctggggatat
    13981 gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctc gcaggcgtta aaaaattacc
    14041 tgaccgagca ccctgaagcg accgatcccc gggattattt gcagtcggct aaatccgcaa
    14101 tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg cgagggcagg gcataacgca
    14161 cctgccattt aacaaggaaa aaacatgaaa acgttaattg cccggcataa agctggtgaa
    14221 catatcggca tatgttcagt ctgttctgcc catccgttgg ttatcgaagc ggcgctggca
    14281 tttgatcgca acagcacgcg caaagtgctg attgaagcaa cgtcaaacca ggtcaatcaa
    14341 tttggcggtt ataccggaat gacaccggca gactttcgcg aatttgtttt tacgattgcc
    14401 gataaagtcg gatttgcacg cgaacgcatt attctcggcg gcgatcatct ggggccaaac
    14461 tgctggcagc aagaaaatgc ggatgcggcg atggaaaaat ccgtcgagct ggtaaaagca
    14521 tatgttcgtg ctggcttcag taaaattcat cttgatgcgt caatgtcctg cgcgggggat
    14581 cccataccgt tagcaccaga aacggttgcg gaacgagctg ctttgctttg ctgcggaaag
    14641 tgtggcgaca gattgccagc gtgagcaact gagctatgtc attggcactg aagttccggt
    14701 tccgggcggt gaggccagcg ccattcagtc agtacacatc acccatgttg aagatgccgc
    14761 caatacttta cgtacgcatc aaaaggcctt tattgcccgt gggctgacag aggcgttaac
    14821 acgcgtgatt gccatcgtgg tgcagccggg cgtggaattt gatcacagca atattatcca
    14881 ttatcagccg caggaagcgc agccgctggc gcaatggata gaaaacaccc gaatggttta
    14941 tgaagcacat tctaccgatt accagacccg gacggcttat tgggaattag tccgcgatca
    15001 ctttgcaata ttgaaagtcg gtcccgcatt aacctttgct ttacgcgagg cgatatttgc
    15061 actggcacaa attgagcagg aacttatcgc ccctgaaaat cgcagcggtt gcctggcggt
    15121 aattgaagaa gtgatgctcg acgaaccgca atactggaaa aaatattatc gtacgggttt
    15181 taacgattca ttactggata ttcgttacag cctgtcggat cgtattcgtt attactggcc
    15241 gcatagtcgg attaaaaata gcgtcgaaac gatgatggtg aatcttgaag gcgtggacat
    15301 cccactgggc atgattagtc agtatcttcc caaacaattt gaacgcattc agtccgggga
    15361 attatcagca ataccgcatc agctgattat ggataaaatt tatgatgttt tgcgcgccta
    15421 tcgctacggc tgtgcggaat aaggacggta tatgactaac ctgtttgttc gtagcggaat
    15481 ttcttttgtc gatcgtagcg aagttttaac ccatatcggt aatgagatgc tcgccaaagg
    15541 tgtggttcat gatacctggc cacaggcatt aattgccaga ga
//
LOCUS       DAZPAC010000087        15407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZPAC010000087 DAZPAC010000000
VERSION     DAZPAC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.087"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..454
                     /locus_tag="ACHUPM_002299"
     CDS             <1..454
                     /locus_tag="ACHUPM_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0870059.1"
                     /translation="GLCMDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRIS
                     LPTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPP
                     PFHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            670..759
                     /locus_tag="ACHUPM_002300"
                     /pseudo
     CDS             670..759
                     /locus_tag="ACHUPM_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(760..1818)
                     /gene="modC"
                     /locus_tag="ACHUPM_002301"
     CDS             complement(760..1818)
                     /gene="modC"
                     /locus_tag="ACHUPM_002301"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR0870060.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1821..2510)
                     /gene="modB"
                     /locus_tag="ACHUPM_002302"
     CDS             complement(1821..2510)
                     /gene="modB"
                     /locus_tag="ACHUPM_002302"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR0870061.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2510..3283)
                     /gene="modA"
                     /locus_tag="ACHUPM_002303"
     CDS             complement(2510..3283)
                     /gene="modA"
                     /locus_tag="ACHUPM_002303"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0870062.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="ACHUPM_002304"
     CDS             complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="ACHUPM_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR0870063.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3727..4515
                     /gene="modE"
                     /locus_tag="ACHUPM_002305"
     CDS             3727..4515
                     /gene="modE"
                     /locus_tag="ACHUPM_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR0870064.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4583..6055
                     /gene="modF"
                     /locus_tag="ACHUPM_002306"
     CDS             4583..6055
                     /gene="modF"
                     /locus_tag="ACHUPM_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR0870065.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6316..7332
                     /gene="galE"
                     /locus_tag="ACHUPM_002307"
     CDS             6316..7332
                     /gene="galE"
                     /locus_tag="ACHUPM_002307"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR0870066.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7342..8388
                     /gene="galT"
                     /locus_tag="ACHUPM_002308"
     CDS             7342..8388
                     /gene="galT"
                     /locus_tag="ACHUPM_002308"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR0870067.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8392..9540
                     /gene="galK"
                     /locus_tag="ACHUPM_002309"
     CDS             8392..9540
                     /gene="galK"
                     /locus_tag="ACHUPM_002309"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR0870068.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9534..10574
                     /gene="galM"
                     /locus_tag="ACHUPM_002310"
     CDS             9534..10574
                     /gene="galM"
                     /locus_tag="ACHUPM_002310"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR0870069.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10777..11529
                     /gene="gpmA"
                     /locus_tag="ACHUPM_002311"
     CDS             10777..11529
                     /gene="gpmA"
                     /locus_tag="ACHUPM_002311"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR0870070.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="ACHUPM_002312"
     CDS             complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="ACHUPM_002312"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR0870071.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13056..13436
                     /locus_tag="ACHUPM_002313"
     CDS             13056..13436
                     /locus_tag="ACHUPM_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR0870072.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13550..14491
                     /gene="zitB"
                     /locus_tag="ACHUPM_002314"
     CDS             13550..14491
                     /gene="zitB"
                     /locus_tag="ACHUPM_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR0870073.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="ACHUPM_002315"
     CDS             complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="ACHUPM_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR0870074.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15245..>15407)
                     /locus_tag="ACHUPM_002316"
     CDS             complement(15245..>15407)
                     /locus_tag="ACHUPM_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="3 different subfamilies; catalyzes the formation of
                     quinolinate from iminoaspartate and dihydroxyacetone
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR0870075.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3916 a         4044 c         3851 g         3596 t
ORIGIN      
        1 cggcctttgc atggatcttg cactggtaac aggacagcgc gaaggagatc tggcggctat
       61 gagatgggag gatataagag atggcaggct atacgtggaa caacaaaaaa caggggcaaa
      121 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      181 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      241 gacaatcgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      301 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      361 cggtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      421 tgacggacga gggagggaat ggaaagacat ttaaaattaa tttcgaatga tttcgaaaca
      481 ccacttgtaa ttaattgatt ataaatgatt aattcctaat gggcatttgc ttcatgaatg
      541 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      601 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      661 aaattcttaa atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
      721 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
      781 tttaatttgc gcgtacagcc acaggccagg tttgatcgcc agttcatccc tggcccacgg
      841 gctgatacgc gcccacagcg ttttaccgcc gacttccagc tccacttcca cctggccgtt
      901 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
      961 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
     1021 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc
     1081 gtaatgcgga tgatgctcaa gtaccgtcac tttcagaatg ctactttgtt gctctttcgg
     1141 cagccacgga ttcatcacgc tactgcccca cacttcctcc agcgcgccaa aggctttcac
     1201 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
     1261 gctgacatac aacatcggaa tgttgatttc ccgtgtcagc cgttgcagat aaggcaacag
     1321 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattccgg
     1381 tgctgtcagc aaagcccgac caatcgccac gcgctgtttt tcgcctccgg acaggctgcc
     1441 tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
     1501 gaccatactt ttcgacatgc cgtagcgcag attgccacgc actttgtaat gcgggaacag
     1561 ccgcgcatcc tgaaaaacat agccaacgcg acgcttttcc ggcgtcaggc agataccttt
     1621 ttcggcatca tttagtaccc gcccattgag gacaatccgc cctttttgcg ggcgcgtcag
     1681 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
     1741 gatgccattg gcgggcagtg tttcattaat ggtcaggcaa tggttgccca acgtctggga
     1801 aaaattcagc tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
     1861 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg
     1921 ccactttccc cgccgggggt ctggatcagg gtatacatgg cagagggaat ggttcgcgtt
     1981 tcgccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca
     2041 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
     2101 aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct gttcgagttt gacatcaacc
     2161 ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg
     2221 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
     2281 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
     2341 aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc
     2401 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
     2461 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
     2521 taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg
     2581 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
     2641 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac
     2701 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
     2761 cttccgccgg agccagtttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
     2821 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat
     2881 tcagcagcga agtccagtta gttgtgctgt cgatgatgaa atctttctgc tcgctggctt
     2941 tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg
     3001 cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag
     3061 gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat
     3121 ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
     3181 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag
     3241 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt
     3301 aacgttatat aggtgacaat gtaacggcta gctcagaatt ttcccagcag ttattgctaa
     3361 cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa
     3421 atgcccgact aagcgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac
     3481 caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg
     3541 ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg
     3601 tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct
     3661 tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac
     3721 attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac
     3781 ccgcgccgga tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg
     3841 aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta
     3901 agcgagcata ttctggttga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg
     3961 acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa
     4021 gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc
     4081 tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc
     4141 gatcatgatg acattcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg
     4201 aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg
     4261 ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac
     4321 gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa
     4381 gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg
     4441 acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc
     4501 gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc
     4561 gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct
     4621 tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc
     4681 gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact
     4741 tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga
     4801 gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc
     4861 tggcgaagat gacaccggac gcactacggc tgaaatcatt caggatgaag taaaggatgc
     4921 accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt
     4981 taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc tgatgtcgga
     5041 gcctgacttg ttgattcttg atgagtcgtt cgatggcctg gatgttgcct cacgtcagca
     5101 gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa
     5161 tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt
     5221 agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcgca
     5281 tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc
     5341 cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg
     5401 ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg
     5461 gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg
     5521 ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga
     5581 tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac
     5641 taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt
     5701 ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg
     5761 tacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg cgctgatcgt
     5821 ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga
     5881 tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac
     5941 gcaattgtta tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct
     6001 tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg
     6061 tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg
     6121 caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt
     6181 ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa
     6241 tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc
     6301 ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat tggaagtcat
     6361 acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac
     6421 agtaagcgca gcgtactgcc tgttatcgag cgcttaggcg gcaaacatcc aacgtttgtt
     6481 gaaggcgata ttcgtaacga agcgttgatg accgagatac tgcacgatca cgctatcgac
     6541 accgtgatcc acttcgccgg gctgaaagcc gtcggcgaat cggtacaaaa accgctggaa
     6601 tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg cgccgctaac
     6661 gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca
     6721 tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag caaattgatg
     6781 gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat tgccctgctg
     6841 cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc
     6901 attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg
     6961 gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac
     7021 gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc
     7081 gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt ggttaatgcc
     7141 ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg cgaaggcgac
     7201 cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg
     7261 cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga
     7321 tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc
     7381 tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg
     7441 cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc
     7501 ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac cgggacttac
     7561 gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac
     7621 gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg
     7681 gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc
     7741 tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac
     7801 aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc
     7861 ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag
     7921 aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt
     7981 gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg
     8041 ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc
     8101 gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc
     8161 ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg
     8221 cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg
     8281 gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag
     8341 cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg
     8401 aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt
     8461 caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga cggtttcgtt
     8521 ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa
     8581 gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc
     8641 attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg
     8701 caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag
     8761 ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag
     8821 ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac
     8881 cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa
     8941 gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc catgccgaaa
     9001 ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac
     9061 aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agctctgcgc
     9121 gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa
     9181 cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag
     9241 caaggcgatc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat
     9301 gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agcagtgatt
     9361 ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgctg
     9421 atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa
     9481 acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga
     9541 acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca
     9601 acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca gctcgtattc
     9661 cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc
     9721 aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata
     9781 gccgttatac ctttgacggt gaaacggtga cgctttcgcc aagtcagggc gttaaccagc
     9841 tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc
     9901 gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg
     9961 gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca
    10021 cagttgataa atcttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg
    10081 agcagtctga cgtgcgcaat cacaagttgc agattctggc gggcgaatat ctgccggttg
    10141 atgaaggcgg cattccgcac gacggcctga aatctgtcgc cggaacctct tttgatttcc
    10201 gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag
    10261 gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg
    10321 tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccc gctcttcaat
    10381 tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct tacgccgact
    10441 ggcaaggact ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc
    10501 cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt
    10561 ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat tgttcaaatc
    10621 accagcaaac accgacatat ttgcaactca atattcacaa caaccttaca ctgcgccact
    10681 attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat ataatgagaa
    10741 ttattatcat aaaaagataa tttgaggagt aagtatatgg ctgtaactaa gctggttctg
    10801 gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg gtacgacgtg
    10861 gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct gaaagaggaa
    10921 ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca taccctgtgg
    10981 aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg gaaactgaac
    11041 gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaaccgccga aaagtatggc
    11101 gacgagcagg tgaaacaatg gcgtcgtggt tttgccgtga ctccgccgga actgactaaa
    11161 gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga gaaagagctg
    11221 ccgctgactg aaagcctggc gctgaccatt gaccgcgtga tcccttactg gaatgaaact
    11281 attctaccgc gtatgaagag cggtgagcgc gtgatcatcg ctgcacacgg taactcttta
    11341 cgtgcgctgg tgaaatatct tgataacatg agtgaagaag agattcttga gctgaatatc
    11401 ccgaccggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct gaaacgctat
    11461 tatctgggta atgctgacga gatcgcagcg aaagcagcgg cggttgctaa ccagggtaaa
    11521 gcgaagtaaa cgtcattcgt ttaaaaatga gaaagccgac tgcaagtgag tcggcttttt
    11581 tgttgctaac aatggagcac attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg
    11641 tgattcatcg gatacgccac tctgacggcg catccgacaa ttaaacctta cccgcgacgc
    11701 gcttttactg cattcgccag ttgacgtaac agagcatcgg tatcttccca gccaatgcag
    11761 gcatcggtga tgctcttacc gtaggccagc ggctccccgc tatcgaggct ctgattgcct
    11821 tccaccagat ggctttccac catcacgcca ataatggcct tttcgccacc ggcaatctgc
    11881 tggcaaacgt cagcacaaac atccatctgc tttttgaatt gtttggacga gttagcatgg
    11941 ctgaaatcga tcatcacctg tgctggcagg cctgctttgt tcagcccttc tttcacttca
    12001 gcaacgtgct tcgcgctgta gttaggctct ttaccgccgc gcagaatgat atggcaatca
    12061 ccgttaccgc tggtattcac aatcgccgaa tgcccccatt tagttacgga caggaagcag
    12121 tgcggcgcac cggcggcatt aatggcatcg atagccactt taatcgtacc gtctgtgcca
    12181 tttttaaaac ctaccggaca agaaagacca gacgccagtt cgcggtgcac ctgcgattcc
    12241 gtggtacgtg caccaattgc gccccagctc atcaggtcag cgagatactg aggagtgatc
    12301 atatcgagaa actcacccgc cgctggcaga ccgctgtcgt taatatcaag cagcaattta
    12361 cgggctatac gcagaccgtc gttgatctgg aagctgttat ccatatgcgg atcgttaatc
    12421 aaccctttcc agcccaccgt ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc
    12481 agctcatctt tcagctcttc acgcagcgcc agcaagcgag tggcatactc tttagccgcg
    12541 acaggatcat gaattgagca cgggccaatc accaccaaca ggcgatcatc attacctttc
    12601 aggatcttat ggatcgcttt tcgggcatgg gcaaccgtat tcgcggcatt ttcagttacg
    12661 gggaattttt ccagcaatgc gacaggagga agtaactctt tgatttcttt gatgcgtaaa
    12721 tcgtcgttct gataattcat gtctgttcca gtgttgccat acttatctta gtgaatgcaa
    12781 tacttccaat ctatatcttc cgtcagaatg tgtaaacggg gttttacact atgaacggat
    12841 taatcctgga attagcaaga aaaacgccag attgtcgcga aaaacgagat ctctcctaca
    12901 atttctaact gtaactcctt tgtttgttaa ttatttcaag attctctgct gcgtttcata
    12961 aactggctga aatcttaaac caatgcctta tattcacctg caaatgcact gttggaagag
    13021 gttatccgac ataacgacca taacaggagc atcctatgaa aatgacaaaa ctggccacat
    13081 tatttctgac tgccactcta agccttgcca gcggtgccgc actggctgcc gatagcggag
    13141 cgcaaactaa taacggccag gcaaacgccg cagctgatgc gggccaggta gcccctgacg
    13201 cccgtgaaaa tgtcgcgcca aataacgtcg acaataacgg ggtaaatacc ggttctggcg
    13261 gcacaatgct gcattcggat ggttcttcaa tgaacaatga cggaatgacc aaagatgaag
    13321 agcacaagaa caccatgtgc aaagatggtc gatgtccgga tatcaataaa aaggtacaaa
    13381 tcggtgatgg catcaataat gatgtcgata ccaaaaccga cggtaccacg cagtaaccct
    13441 gcaaaatagc caataatggg agagcggagt ctctcccttt taatttttca cttctaaaag
    13501 cagtatgatg tcataaagtt aagtgagaat aattaaggga tgactcgtca tggcgcactc
    13561 acactcacac acgtcctcac acctgccaga agataataat gctcgtcgct tgttgtatgc
    13621 tttcggcgtg actgccgggt ttatgctggt agaagtcgtt ggtggttttc tttctggttc
    13681 tctggcattg ctggccgatg cgggtcatat gttgaccgat actgccgccc tgcttttcgc
    13741 cctactcgcc gtgcaatttt cacgtcgtcc tcccaccatt cgccacactt tcggttggct
    13801 aagactgacc acgctcgcgg cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt
    13861 gattgtctgg gaggcgatag aacgcttccg cacgccgcgt ccggtcgagg gcggcatgat
    13921 gatggcaatt gccgtggctg ggttgctggc aaatatactt tctttctggt tacttcatca
    13981 cggcagcgaa gagaaaaacc tcaacgtacg agcagcggca ttgcatgtgc tcggggacct
    14041 gttgggttcg gttggggcta ttattgccgc gttgattatt atctggaccg gctggacacc
    14101 tgctgacccc attctctcga tagtggtgtc gcttctggtt ctgcgtagcg cgtggcgact
    14161 gttgaaagat agcgtgaatg aattacttga aggtgcaccg gtatcgctgg atatcgctga
    14221 actgaagcgt cgtatgtgcc gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt
    14281 atggatggta ggcgagaagc cggtgataac gctgcatgtg caggtgatcc cgccacacga
    14341 tcacgatgcc ttgttggatc agatccaaca ctacctgatg gatcactatc agattgagca
    14401 cgccaccatt cagatggaat atcaaccttg tcatgggcca gactgccacc tgaatgaagg
    14461 ggtgtctggc cattcacatc atcaccatta atgggacagc gcgcgtgagc ctctttcacg
    14521 tgcgctgttg atccacatcc gactgccgtt gagcgcaata aaggtcagga tgatgtactc
    14581 cagagacatg gcgtaaacgc cctgaagtgc aaaaataacg acgctaatca cgttaataat
    14641 cacccacaac agccagtttt ccacatactt acgcgtcatc agaatcattg ccacgataga
    14701 taacaccatc atgcatgaat cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac
    14761 aacctgtaat cctaatgctt gcatgatcat gactgccacg cgggtcaaaa atgcaaacac
    14821 cggattgata aataccgtca tcagaccaat cgaaacaacg caaaccgcca accagctgag
    14881 cgccttcggc aatggtaacc agcgaatttt caactccgcc tcgttctgac tggtttgtcg
    14941 cgaccacgca taccaaccgt aaatattcgc ggcaaagaaa aacacctgta atagcaggct
    15001 ggcatacagc tgaatctgaa agaaaataat gccaaacaag gtgacgttga tcaggccaaa
    15061 gaagtagttg ctgatcttct ccagactggc aaggccaata cacagcaacc cggcgatcgt
    15121 gcctaccgct tcgatccatg agagatcata accgcctgcc cctattggta tatgtaccag
    15181 gatattctgc acactaaaaa aatccatctt tcccccaaag cgtaacgtta cgccttatta
    15241 ttcgttatcc acgtagtgta gccgcaaaat ccagcatacg attgagcggc accagcgccc
    15301 tctctcgcag cctttcatca agaacctcgt gattgcttcc ttcctgttct aatgcctctg
    15361 cgatggactg aaggccattc atggccatcc acggacaatg cgcgcag
//
LOCUS       DAZPAC010000088        15366 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZPAC010000088 DAZPAC010000000
VERSION     DAZPAC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.088"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            83..2617
                     /gene="mrcB"
                     /locus_tag="ACHUPM_002317"
     CDS             83..2617
                     /gene="mrcB"
                     /locus_tag="ACHUPM_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR0870076.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2837..5026
                     /gene="fhuA"
                     /locus_tag="ACHUPM_002318"
     CDS             2837..5026
                     /gene="fhuA"
                     /locus_tag="ACHUPM_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR0870077.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            5077..5874
                     /gene="fhuC"
                     /locus_tag="ACHUPM_002319"
     CDS             5077..5874
                     /gene="fhuC"
                     /locus_tag="ACHUPM_002319"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR0870078.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5874..6764
                     /gene="fhuD"
                     /locus_tag="ACHUPM_002320"
     CDS             5874..6764
                     /gene="fhuD"
                     /locus_tag="ACHUPM_002320"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR0870079.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6761..8743
                     /gene="fhuB"
                     /locus_tag="ACHUPM_002321"
     CDS             6761..8743
                     /gene="fhuB"
                     /locus_tag="ACHUPM_002321"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR0870080.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8901..10181)
                     /gene="hemL"
                     /locus_tag="ACHUPM_002322"
     CDS             complement(8901..10181)
                     /gene="hemL"
                     /locus_tag="ACHUPM_002322"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR0870081.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10347..10394
                     /gene="yadX"
                     /locus_tag="ACHUPM_002323"
     CDS             10347..10394
                     /gene="yadX"
                     /locus_tag="ACHUPM_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR0870082.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            10406..11827
                     /gene="clcA"
                     /locus_tag="ACHUPM_002324"
     CDS             10406..11827
                     /gene="clcA"
                     /locus_tag="ACHUPM_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR0870083.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11851..11916
                     /gene="yadW"
                     /locus_tag="ACHUPM_002325"
     CDS             11851..11916
                     /gene="yadW"
                     /locus_tag="ACHUPM_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR0870084.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            11909..12253
                     /gene="erpA"
                     /locus_tag="ACHUPM_002326"
     CDS             11909..12253
                     /gene="erpA"
                     /locus_tag="ACHUPM_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR0870085.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12300..12923)
                     /locus_tag="ACHUPM_002327"
     CDS             complement(12300..12923)
                     /locus_tag="ACHUPM_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR0870086.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12961..13761)
                     /gene="btuF"
                     /locus_tag="ACHUPM_002328"
     CDS             complement(12961..13761)
                     /gene="btuF"
                     /locus_tag="ACHUPM_002328"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR0870087.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13754..14452)
                     /gene="mtnN"
                     /locus_tag="ACHUPM_002329"
     CDS             complement(13754..14452)
                     /gene="mtnN"
                     /locus_tag="ACHUPM_002329"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR0870088.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14536..>15366
                     /gene="dgt"
                     /locus_tag="ACHUPM_002330"
     CDS             14536..>15366
                     /gene="dgt"
                     /locus_tag="ACHUPM_002330"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR0870089.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     L"
BASE COUNT         3471 a         3864 c         4287 g         3744 t
ORIGIN      
        1 attttgagag atatcttctt ctgtcttgta acagaagaac agaaaatcgg gcttttgcgc
       61 ctgaatattg cggagaaaaa gcatggccgg gaatgaccgc gagccaattg gacgcaaagg
      121 gaaaccgacg cgtccggtca aacaaaaggt aagccgtcgt cgttacgaag atgacgatga
      181 ttacgacgat tatgatgact atgaggatga agaaccgatg ccgcgcaaag gtaagggcaa
      241 aggcaaaggg cgtaagcctc gtggcaaacg cggctggcta tggctgctgt taaaactggc
      301 tatcgttttt gccgtgctga tcgccattta cggcgtttat ctcgatcaaa aaattcgtag
      361 ccgtattgat ggcaaggtct ggcaactgcc cgcggcagtt tatggccgaa tggtcaacct
      421 tgaaccagat atgaccatca gcaagaacga aatggtgaag ctgctggagg caacacagta
      481 tcgtcaggtg tcgaaaatga cccgtcctgg cgaatttacc gtgcaggcca acagcattga
      541 gatgatccgc cgtccgtttg atttcccgga cagcaaagaa gggcaggtgc gtgcgcgtct
      601 gacctttgat ggcgatcatc tggcgacgat cgtcaatatg gagaacaacc gtcagttcgg
      661 tttcttccgt cttgatccgc gcctgatcac catgatctct tcgccaaacg gtgagcagcg
      721 tctgtttgtg ccgcgcagtg gtttcccgga cttgctggtg gatactttgc tggcgacaga
      781 agaccgtcat ttctacgagc atgatgggat cagtctctac tccatcggac gtgcggtgct
      841 ggcaaacctg accgccggac gcacggtgca gggagcgagt acgctgacgc aacagctggt
      901 gaaaaacctg ttcctctcca gcgagcgttc ttactggcgt aaagcgaacg aagcttacat
      961 ggcgctgatc atggacgcgc gttacagcaa agaccgtatt cttgagctgt atatgaacga
     1021 ggtgtatctc ggtcagagcg gcgacaacga aatccgcggc ttcccgcttg cgagcttgta
     1081 ttactttggt cgcccggtag aagagctcag cctcgaccag caggcgctgt tagtcggtat
     1141 ggtgaaaggg gcgtccatct acaacccgtg gcgtaacccg aaactggcgc tggagcgacg
     1201 taatctggtg ctgcgtttgc tacaacaaca gcagattatc gatggtgaac tgtatgaaat
     1261 gctcagcgcg cgtcctctgg gcgtacagcc gcgcggtggg gtaatctctc cacagccagc
     1321 ctttatgcaa ctggtgcgtc aggagctgca ggcgaaatta ggcgataagg taaaagatct
     1381 ctccggcgtg aagatcttca ctacctttga ctcggtggcc caggacgcgg cagaaaaagc
     1441 cgccgtggaa ggcattccgg cactgaagaa acagcgtaag ttgagcgatc ttgaaactgc
     1501 aattgtggtc gtcgaccgct ttagtggtga agttcgtgcg atggtgggag gttctgagcc
     1561 gcagtttgcg ggctacaacc gtgcgatgca ggcgcgtcgt tcgattggtt cccttgcaaa
     1621 accagcgact tatctgacgg ctttaagcca gccgaaaatc tatcgcctga acacgtggat
     1681 tgccgatgcg ccaatcgcgc tgcgtcagcc gaatggccag gtctggtcac cgcagaacga
     1741 tgaccgtcgt tatagcgaaa gcggcagagt gatgctggtg gatgcgttga cccgttcgat
     1801 gaacgtgccg acggtaaatc tggggatggc gctggggctg cctgcagtta cggagacctg
     1861 gatcaaactg ggcgtgccga aagatcagct gcatccggtt ccggcgatgc tgctgggggc
     1921 gttgaactta acgccaatcg aagtggcgca ggcattccag accatcgcca gcggcggtaa
     1981 ccgtgcaccg ctttctgcac tgcgttcggt aatcgcggaa gatggcaagg tgctgtatca
     2041 gagcttcccg caggcagaac gcgctgttcc ggcgcaggcg gcgtatctga cactatggac
     2101 catgcagcag gtggtacaac gtggtacggg tcgtcaactt ggggcgaaat acccgaacct
     2161 gcatctggca gggaaaacag ggactaccaa caataacgta gatacctggt ttgcgggcat
     2221 tgacggcagc acggtgacca tcacctgggt aggccgtgat aacaaccagc cgaccaaact
     2281 gtatggtgcc agcggggcaa tgtctattta tcagcgttat ctggctaacc agacgccaac
     2341 gccgctgaat cttgttccgc cagaagatat tgcagatatg ggcgtggact acgacggcaa
     2401 ctttgtttgc agcggtggca tgcgtgtctt gccggtctgg accagcgatc cgcaatcgct
     2461 gtgccagcag agcgagatgc agcagcagcc gtcaggcaat ccgtttgatc agtcttctca
     2521 gccgcagcaa cagccgcaac agcaacctgc tcagcaagag cagaaagaca gcgacggtgt
     2581 agccggttgg atcaaggata tgtttggtag taattaacat ctaagcgtga aataccggat
     2641 ggcgaattgc catccggtaa aataacatcc cacctaaagt attaaccctt tcttttcatc
     2701 tggttgttta ttaacccttc aggaacgctc agattgcgta ccgcttgcga acccgccagc
     2761 gtttcgaata ttatcttatc tttataataa tcattctcgt ttacgttatc attcacttta
     2821 catcagagat ataccaatgg cgcgttccaa aactgctcag ccaaaacact cactgcgtaa
     2881 aatcgcagtt gtagtagcca cagcggttag cggcatgtct gtttatgcac aggcagcggt
     2941 tgaaccgaaa gaagacacta tcaccgttac cgctgcacct gcgccgcaag aaagcgcatg
     3001 ggggcctgct gcaactattg cggcgaagca ctctgctact gcgactaaaa cggatacacc
     3061 aattgaaaaa acgccgcagt ctatttcggt tgtgacaaat gaagagatgc agatgcgtca
     3121 atttcagtct gtaaaagaag cattaggtta tacacctggc gttacggtta gcagtcgtgg
     3181 tgcttctaat acatatgatt ttgttatcat tcgtggtttc tcatctgttg gtctgaatca
     3241 aaataattac cttgatggac taaaacttca gggcaacttt tataacgatg ctgtgattga
     3301 tccttacatg ctcgagaggg ttgaactgat gcgtgggccg acgtctgttc tctatggaaa
     3361 aagtaatcct ggtgggatta tttctatggt gagtaagcgt ccgaccactg aacccctgaa
     3421 agaaattcag tttaaaatgg ggacggataa tctgtttcag accggattcg atttcagtga
     3481 tgcactggat gataacggtg agttctctta ccgtttgacg ggcctcgctc gctcaacaaa
     3541 cgaacagcag aaaaactctg aatctcagcg ttataccatt gctccatcat tctcatggcg
     3601 tccagacgac aaaaccaatt ttactttcct gtcctatttc cagaatgaac cagaaacggg
     3661 ttattacggt tggttgccga aagaggggac cgttgagcca ttgcctaatg gtaagcgtct
     3721 accgactgac ttcaatgaag gtgcatcgaa taatacatac tcccgtaacc agaaaatggt
     3781 gggatatagt tttgaacatg gttttaatga caccttcacc gtgcgtcaga atctgcgttt
     3841 cagtgaaatg aaaacctcac agaaaagtgt ttatggcaca gggattgctg gtgatggtca
     3901 taccctaaac cgcgggacag tggtggataa tgagcgtcta caaaacttta gcgttgatac
     3961 ccaacttgaa agtaagtttg ctacaggtga cgtcgagcat actttgctga caggggtaga
     4021 cttcatgcgg atgcgtaatg atatcaatgc cagctttgga tccgcaccat ccatcgatct
     4081 ttataacaaa tatcaccctg aatactttgc atttggtagc gcagagccat accagatgaa
     4141 tgaaagcaaa caaacaggta tttatgttca ggatcaggcg gaatggaata aatgggtatt
     4201 cactctgggg ggacgctacg attggtctaa gcaagcgacc actgttcgtg aaaactctta
     4261 tacgccgact gaaggttata ttgagcgcaa tgatcatcag ttcacctggc gcggtggtgt
     4321 aaattactta ttcgataatg gtatttcacc ttactttagc tatagccagt cctttgaacc
     4381 gagtgctttc gatctgtgga gcaacccgcg catttcctat aagccatcga aaggtgaaca
     4441 gtatgaagct ggcgtaaaat atgttccgaa tgatatgccg gtcgttgtta cgggcgcagt
     4501 ctatcaattg acgaaaacaa ataacctgac agcagaccca acaaacccat tagcgcaagt
     4561 cccagcaggt gagattcgcg ctcgtggtgt ggaacttgaa gcaaaagcgg cgttaaatgc
     4621 caatattaac ttgacggctt cttataccta caccgatgcg gaatacacca aagacaccaa
     4681 tctcaaaggt aaaactccag aacaagtacc ggagcatatg gcatctctct ggggggatta
     4741 taccttcaat gaagggccgc tttctggttt aacattggga acaggtggtc gttttattgg
     4801 ttccagctat ggtgatccgg caaacacttt taaagtgggt agcacagctg taatggatgc
     4861 tgttgtaaaa tatgatctgg cacgctttgg tatggcggga tccagccttg ctgtgaacgt
     4921 caacaatttg ctcgatcgtg agtatgttgc cagttgcttc cagacttatg gctgcttctg
     4981 gggcgcagaa cgtcaggtcg ttgcaaccgc aaccttccgt ttctaatttc tcttttggga
     5041 cacggatttc cgtgcccatt tcacaagttg gctgttatgc aggaatacac gaatcattcc
     5101 gataccactt ttgcactgcg taatatctcc tttcgtgtgc ccgggcgcac gcttttgcat
     5161 ccgctgtcgt taacctttcc tgccgggaaa gtgaccggtc tgattggtca caacggttct
     5221 ggtaaatcca ctctgctcaa aatgcttggt cgtcatcagc ctccgtcaga aggggagatt
     5281 cttcttgatg cccagccgct ggaaagctgg agcagcaaag cgtttgcccg caaagtggct
     5341 tatttgccgc agcagcttcc tccggcagaa gggatgaccg tgcgtgaact ggtggcgatt
     5401 ggtcgttacc cgtggcatgg cgcgctgggg cgctttggtg cggcagatcg cgaaaaggtc
     5461 gaggaagcta tctcgctggt tggcttaaaa ccgctggcgc atcggctggt cgatagtctc
     5521 tctggcggcg aacgtcagcg ggcgtggatc gccatgctgg tggcgcagga tagccgttgt
     5581 ctgttgctcg acgaaccgac ttcggcgctg gatatcgccc accaggttga tgtgctggcg
     5641 ctggtgcacc gtttaagtca ggaacgtggc ctgacggtca ttgccgtgtt gcacgatatc
     5701 aatatggcgg cacgctactg tgattatctg gtcgccctgc gcagtggtga aatgattgct
     5761 cagggaacgc ctgcggaaat tatgcgcggc gaaaccctcg aaatgattta tggcatcccg
     5821 atgggtattt tgccgcatcc ggcgggtgct gcacctgtga gttttgttta ttgatgagcg
     5881 gcttacctct tatttcgcgc cgtcgactgt taacggcgat ggcgctttct ccgttgttat
     5941 ggcagatgaa taccgcccac gcggcggcta ttgaccccaa tcgtattgtg gcgctggagt
     6001 ggttgccggt ggaattactg ctggcgctcg gcatcgtgcc ttacggcgtg gcggatacca
     6061 tcaactatcg cctgtgggtc agcgaaccac cattgccgga ctcagtgatt gacgtcggtt
     6121 tgcgtacaga acctaacctt gaactgctga ccgaaatgaa accgtcgttt atggtctggt
     6181 cggcaggata tggcccttca tcagaaatgc tggcacgtat tgcgccgggt cgcggattta
     6241 acttcagtga cggcaaacag ccgttggcga tggcgcgtaa atcgctgacg gaaatggcag
     6301 atttacttaa cctgcaaagc gcagcggaaa cgcatttagc gcaatacgaa gactttatcc
     6361 gcagcatgaa accccgcttt gtgaagcgtg gtgcacgccc gttactgctg acgacgctta
     6421 tcgatccgcg ccatatgctg gtcttcggtc caaacagctt gttccaggaa attctcgatg
     6481 agtacggcat cccaaatgcc tggcaagggg aaaccaactt ctggggcagt accgccgtca
     6541 gtatcgatcg tctggcagcg tataaagacg ttgatgtgct ctgttttgat cacgacaaca
     6601 gcaaagacat ggatgcgcta atggcaaccc cgctgtggca ggccatgccg tttgtccgcg
     6661 ccggacgctt tcagcgcgta cctgcagtct ggttttatgg tgcgacactc tcggcaatgc
     6721 actttgtgcg cgttctggat aacgccatcg gaggtaaagc gtgagtaaac gaattgcgct
     6781 ttttccggcg ttattgctgg cgctgttagt gattgtcgct acggcgctca cctggatgaa
     6841 cttctcgcag gcgctgccgc gtagccagtg ggcgcaggct gcctggtcgc cagacatcga
     6901 tgtcatcgag cagatgattt ttcactacag cttgttgccg cgtctggcga tttcgctgct
     6961 ggtgggcgcg ggcctggggc tggtgggcgt gctgtttcag caagtgctgc gtaacccgct
     7021 ggcggagccg acgacgcttg gcgttgctac aggcgcgcaa ctgggaatta ccgtcaccac
     7081 actttgggcg atcccaggtg ctatggcgag ccagtttgcc gcactggtgg gcgcttgtgt
     7141 cgttggctta atcgtctttg gcgtcgcgtg ggggaaacgg ctttcgccgg ttacgcttat
     7201 tctcgcgggg ctggtggtga gcctttattg cggggcaatt aatcagctac tggttatctt
     7261 ccatcacgac cagctgcaaa gcatgttcct gtggagcacc ggaacgctga cgcaaaccga
     7321 ctggggcggc gttgagcgtt tatggccgca gttgctgggc ggcgtgatgt tgacgttatt
     7381 gctacttcgc ccgttaaccc tgatggggct tgatgatggc gtggcgcgca atctcgggct
     7441 ggccttgtcg ctcgcgcgtc tggcggcgct gtcgctggcg atagtcatca gtgcgctgct
     7501 ggtgaatgcg gtggggatta tcggctttat cggtttgttc gcgccgctgc tggcgaaaat
     7561 gctgggggcg cggcgtttgt tgccacggtt gatgctggcg tcgctgattg gggcgctgat
     7621 cctctggctt tccgatcaaa tcatcctctg gctgactcgc gtgtggatgg aagtgtccac
     7681 cggttcggtc actgcgttga tcggtgcgcc gctgctactg tggctgttgc cgcgtttacg
     7741 cagcattagc gcgccggata tgaaggtcaa cgatcgtgtc gcggctgaac gccaacatgt
     7801 gctggcgttt gccctcgcgg gcggcgtgct gctgttgatg gctgtggtgg tggcgctgtc
     7861 gtttggtcgt gatgcgcacg gctggacgtg ggcgagcggg gcgttgctcg aggatttgat
     7921 gccctggcgc tggccgcgaa ttatggcggc gctgtttgcg ggcgtcatgc tggcggtggc
     7981 gggctgtatt attcagcggc tgaccggaaa cccgatggca agcccggaag tgctggggat
     8041 tagctctggc gcggcgtttg gcgtggtgtt aatgctgttt ctggtgccgg gtaatgcctt
     8101 tggctggctg ttacccgcgg gcagtatcgg ggcggcggtg acgctgttga tcattatgat
     8161 cgccgccggc cgcggtggat tttccccaca ccgtatgtta ctggcgggga tggcgttaag
     8221 taccgcattc accatgcttt tgatgatgtt gcaggcgagc ggtgacccgc gaatggcgca
     8281 agtgctgacc tggatttccg gttcgaccta caacgcgacc gatgcgcagg tctggcgtac
     8341 cggaattgtg atggtgattt tgctggcgat taccccgctg taccgccgct ggctgaccat
     8401 tttaccgctg ggtggtgata ccgcacgagc cgtaggaatg gcgctgacgc cgacgcgaat
     8461 tgcgctgctg ctgttagcgg cttgcctgac ggcgaccgcg acgatgacta ttggaccgtt
     8521 gagttttgtt ggtttaatgg caccgcatat tgcgcggatg atgggctttc gacggacgat
     8581 gccacacatc gtaatttcgg cgctggtggg tggtttactg ctggtattcg ctgactggtg
     8641 tgggcggatg gtgctgtttc cattccagat cccggcgggg ctgctgtcga cctttatcgg
     8701 cgcgccatat tttatctatt tgctgagaaa gcagagccgt taatttttcc atataacggc
     8761 cctgtacgcc tggacggata aagcgtttac gcgacaacgg atgcccgatg cgacgctggc
     8821 gcgtcttatc gggcctacaa acggccccga atcgtaggtc ggataaggcg ttcacgccgc
     8881 atccgacaaa ccatgctgga tcacaacttc gcaaacaccc gacgtgcagc atcgatggtg
     8941 ttattgatat cttccatgct gtgcgccacg gacataaagc ccgcttcaaa cgctgacggt
     9001 gccaggtaaa caccttcgtc cagcatcata tggaagaaac gcttaaagcg ttccacgtca
     9061 caggccatca catcctgata gcacgtcacg gactcggcgt cggtaaagaa aataccgaac
     9121 atgccgccaa cgtggttaac gaccagcgga attccggctt cttctgccgc ttccagcaga
     9181 ccttctgcca gacgtgttgt cagctcatcc aacgtttcgt gaacgcccgg ttgcgcgact
     9241 tcattcagac aggcgaaacc ggctgccatc gcaattgggt taccggaaag cgtacccgcc
     9301 tgatagaccg gacccgtcgg ggccagcgca tccattacat cacgacgacc accgaatgcg
     9361 cctaccggca ttccaccgcc gatgattttg cccaggcagg tgagatccgg ttccacaccg
     9421 taataatcct gtgcgccagc cagtgccacg cggaagccgg tcattacttc atcgatgatc
     9481 agcaatgcgc caaattcgtc gcacagcgca cgcagacctg gcaggaagtc tggcagcggt
     9541 ggaacacagt tcatattgcc tgccaccggc tcgatgataa tacaggcaat ctcttgcggg
     9601 tattgctcaa acgcggcgcg tacagaagcc agatcgttat aagtacaggt taaggtatgt
     9661 ttggcgaaat ctgccggaac gcccggcgag tttggctggc ctaacgtgag tgcgccagaa
     9721 ccggctttca ccagcaggca gtcagcgtga ccgtggtaac acccttcaaa tttaatgatt
     9781 ttgtcgcgac cggtaaaacc acgggccaga cggatggcgc tcatcgtcgc ctcggtgccg
     9841 gagttcacca tgcgcaccat atccatggtc gggaccaatt cggtcaccag ttgcgccatt
     9901 ttcacttcca tttcggttgg tgcaccaaag cttaaaccac gctcggcggc ttcaatcacg
     9961 gcattgcgga ttgccggatg gttatggccc agcaccatcg gcccccagga accgacataa
    10021 tcgatatagg ctttgccatc aacatcgtac agataagcgc cgtccgcttt ttcgataaac
    10081 agtggagtgc cgcccacgcc agtaaaggcg cgaacagggg agttcacacc gccagggatc
    10141 agctcgcgcg ctgcgctgta aagattttca gacttactca tggagggttc ctgattcgta
    10201 gaaaaagtga atggctgcta ttctatgtta ttcataacaa gttaaatact cgtcaaacat
    10261 caggctgctt gtactggtgc aatcctgatt tcttagagta taaaagtttt gtgcatttga
    10321 aacattacgc tttgcaaagg attttcatgg aacgtgcgag taaaatgccg tcatcttatt
    10381 tgtatgacca ataagtgatc attggatgaa aactgatact ccctctttag aaacaccgca
    10441 ggccgcgcgc ctgcgacgca gacaactgat tcgccaactt cttgagcgcg ataaaacccc
    10501 gttagccatt ttgtttatgg cggcagtcgt cggcacgctt gttgggctgg cagcggttgc
    10561 ttttgacaaa ggtgtcgcct ggttgcagaa ccaacgtatg ggggcgctgg tacatactgc
    10621 tgataattat ccgcttctgt taaccgtcgc ttttctctgt tcggcggtgc tggcgatgtt
    10681 tggctacttt ttggtgcgca aatacgcgcc ggaagcaggt ggttcgggga tcccggaaat
    10741 tgaaggggcg ctggaagatc aacgtcccgt tcgctggtgg cgtgtattgc cggtgaagtt
    10801 ctttggcggg ctggggacac tcggcggagg catggtgttg gggcgcgaag ggccaaccgt
    10861 gcagatcggc ggtaacattg gccgtatggt gcttgatatt ttccgcctga aaggtgacga
    10921 agctcgccat acgctgctgg caaccggtgc tgctgcgggg ctggctgcgg cctttaacgc
    10981 gccgctggcg ggtattttgt ttattatcga agagatgcgt ccgcagtttc gctatacgtt
    11041 aatttcgata aaagcggtat ttattggcgt cattatgtcg accattatgt accggatttt
    11101 taatcatgaa gttgcgttga ttgacgtcgg taaactttct gatgcgccgc ttaatacgct
    11161 gtggctttat ctgatcctcg gtattatttt tggcattttc ggccctattt ttaataaatg
    11221 ggtgctgggg atgcaggatt tgctgcaccg tgtgcacggc ggcaatatta ccaaatgggt
    11281 actgatgggc ggtgcgattg gcggtctgtg tggattgctg gggtttgtgg caccagcaac
    11341 gtcgggcggc ggttttaacc tgattcctat cgctaccgcg gggaatttca gcatgggaat
    11401 gctggtgttt atcttcgtcg ctcgggtcat taccacctta ctctgcttct cttccggcgc
    11461 gccgggcggt atttttgccc cgatgctggc gctgggtact gtgctgggaa ccgctttcgg
    11521 aatggttgcc gttgagctgt ttccgcaata tcaccttgag gcggggacgt ttgctattgc
    11581 cggaatgggg gcattactgg cggcatctat tcgcgcgccg ttaacgggga tcattctggt
    11641 tctggagatg accgataact accagctcat tttgccaatg attattaccg gtcttggcgc
    11701 aacactatta gcgcaattta ccggcgggaa accgctatac tcggcgattc ttgcgcgcac
    11761 gctggcaaaa caggaagctg agcaactggc gcgaagcaag gccgcatcag ccagcgagaa
    11821 tacttgaacg aaataccagg gtattagata atggcgatta ttattgggtt agaatttgcc
    11881 caattgccga tgtcgtttgg agcaaaatat gagtgatgac gtagcactgc cgctggagtt
    11941 taccgacgca gcagccaaca aagttaaaag cctgatcgct gacgaagata acccgaatct
    12001 gaaattacgc gtgtatatca ccggtggcgg ttgcagcggc ttccagtatg gtttcacctt
    12061 tgatgatcag gtgaacgaag gcgatatgac catcgaaaaa cagggcgttg gcttggtggt
    12121 tgatccgatg agcctgcaat atctggtcgg cggttccgtt gactataccg aaggactgga
    12181 aggttctcgt ttcatcgtga ccaacccgaa cgcgaaaagc acctgcggtt gcggttcttc
    12241 ctttagcatc taatctgttg ttcatgtgcc ggataaacta tccggcgcat cttctctctt
    12301 caacgcccat tctcatccag cgcaaacgtc ggtagcttaa gatgccaacg aatagccgcc
    12361 agccgaatca atagcgtcac gaccatgccc atcatactgg ctgtttccag tggtacggaa
    12421 aatgtgtaat aagccgtagc gtggacaata ccgccgataa tacaggcagt tgcgtagatt
    12481 tctgtacgta aaatcatggg gatctcgcgg gccagtacat cacgaatgat cccgccgcca
    12541 acgccagtaa tgacgaccat acaaaccgcg attaacggac cgacttccgc attaaaggct
    12601 ttattcacgc caatgccgac aaacaccgcc agaccaacgg cgtccagcac cggcaacatc
    12661 cattttggta agcgtcttgg ctggcgcacc agcacgatgg tcagcatgct ggttaccatt
    12721 gcaacgacca gatcggtggg atctttcacc caaaataccg ggccgtgatc cagcgccatg
    12781 tcgcgaattg tcccgccgcc tacagcggta accacgccca gtaccagaac accaaaaggg
    12841 tccatacgca attttccggc taacaaaacg ccggagatgg caaataccgc tgtgccgact
    12901 atatccagcc aatagacgag cattgttcaa tccccactga gcacctaaaa aggtcaggcg
    12961 ctaatctacc tgtgaaagcg cattacagag ctgttgtgca gcgaggataa tacgtgggct
    13021 tgcacgttca aaccagtcac tcgtgagagg aataacggga attttgagct gttcacccca
    13081 gtattgtttg attttaggaa tttggtccgg tccgcctgta atgacaatcg cctgtggcga
    13141 gcgtgctaac acctgttcgc ggctaacttg cggccaggga acccggctgt ctttaaagat
    13201 gttttctccg ccacaaactt cgagtacctg gttttgaatc gactcttttc cactggtaaa
    13261 tggcggatta atgccgaatt gcagaaaaac acgtttttta ggtttatcag catattgcgc
    13321 tttcaattgc gcgtactgat ccagcaggga ttgcgcggct tgttcggcct tatccggttg
    13381 cggactccag ggggccaatt gacgtaacgc attggcaatt tgttcaatgc ttgtcgcatc
    13441 gacccacatc acttttattc ccagcgaagc cagctggtca acctgccgct cggcattacc
    13501 tccacgccag gcaatcacca gatcgggttt cagcgcgaca atgcgttcca gattcatccc
    13561 ctgccaggtg gaaacctgct caatcttttg cgcttgtgga ggatagtcgg aatagctgct
    13621 gaccccaacc ggcgtgatcc cggcggcaaa ggcaagttca gtgttggcgg gagaaagcgt
    13681 gatgacgcgc ggcgcggcgt tgagccacag tggcgcaaga aaagacaggg cgaccagcgc
    13741 cctgaacagt gacttagcca tgtgccagtt tctgcaccag tgactcaacc atcaggctgg
    13801 actgtttagc ggcaacagcc aggaactcat cgaagctaag atgagactgt tgatcggcca
    13861 cgtcggagat ggcgcgtacg acaacaaacg ggacgttgaa attgtggcag acatgggcga
    13921 ttgccgtcgc ttccatctct acagcaatgg cctgtgggaa gttgtggcgg attttcgcca
    13981 gaccaacaga accgttgatg aaagcgtcgc cgctaacaat caggccacgt acagcgttaa
    14041 gattcagttc ggcaatgcag gcctcagcgg cagcgatcag tttatcgtca gctttaaagc
    14101 ctgccggaca gcctggtaac tgaccgtatt cataaccaaa tgccgtgaca tccgcgtcgt
    14161 gataacgcgc ttcgtccgag acaacgatat cgcccacttt cagcgttggt gccaggccac
    14221 cggcagaacc ggtgttaata atcacatctg gcttgcagtg ttccaacagc agagtggcac
    14281 ccagcgccgc agcgacttta ccgatgcccg atttcagaag cgcaacctcg gttccattca
    14341 gttggccggt atagatttcg caaccgccga gactgatagt ttgacggttt tcgattttgt
    14401 cacgcagcag cgtaacttct tcttccattg caccaatgat gccgattttc atagatttac
    14461 tcgcgataag cccgatttga aagcatagtt taccatgcgc ttacggggaa gcgtatttct
    14521 cacgcgggag aggacatggc acagattgat ttccgaaaaa aaataaactg gcatcgtcgt
    14581 taccgttcac cgcagggcgt taaaaccgaa catgagatcc tgcggatctt cgagagcgat
    14641 cgcgggcgta tcatcaactc tccggcaatt cgtcgtctgc aacaaaagac ccaggttttt
    14701 ccactggagc gcaatgccgc cgtgcgcacg cgtcttaccc actcgatgga agtccagcag
    14761 gtgggacgct acatcgccaa agaaatttta agccgtctga aagagcttaa attgctggaa
    14821 gcatacggcc tggatgaact gaccggcccc tttgaaagca ttgttgagat gtcatgcctg
    14881 atgcacgata tcggcaatcc gccgtttggt cattttggcg aagcagcgat aaatgactgg
    14941 tttcgccaac gtttgcaccc ggaagatgcc gaaagccagc ctctgactga cgatcgctgc
    15001 agcgtggcgg cactacgttt acgggacggg gaagaacagc ttaacgagct gcggcgcaag
    15061 attcgtcagg acttatgtca ttttgagggg aatgcacaag gcattcgtct ggtgcataca
    15121 ttgatgcgga tgaatctcac ttgggcacag gttggcggta ttttaaaata tacccgtccg
    15181 gcgtggtggc gtggcgaaac gcctgagaca catcactatt taatgaaaaa gccgggttat
    15241 tatctttctg aagaagccta tattgcccgg ttgcgtaaag aacttaattt ggcgctttac
    15301 agtcgttttc cattaacgtg gattatggaa gctgccgacg acatctccta ttgtgtggca
    15361 gacctt
//
LOCUS       DAZPAC010000089        15337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZPAC010000089 DAZPAC010000000
VERSION     DAZPAC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.089"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            99..851
                     /locus_tag="ACHUPM_002331"
                     /pseudo
     CDS             99..851
                     /locus_tag="ACHUPM_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
     gene            complement(892..1824)
                     /locus_tag="ACHUPM_002332"
     CDS             complement(892..1824)
                     /locus_tag="ACHUPM_002332"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR0870090.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2188..4974
                     /gene="polA"
                     /locus_tag="ACHUPM_002333"
     CDS             2188..4974
                     /gene="polA"
                     /locus_tag="ACHUPM_002333"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR0870091.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5356..5988)
                     /gene="yihA"
                     /locus_tag="ACHUPM_002334"
     CDS             complement(5356..5988)
                     /gene="yihA"
                     /locus_tag="ACHUPM_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR0870092.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6285..6451
                     /locus_tag="ACHUPM_002335"
                     /pseudo
     CDS             6285..6451
                     /locus_tag="ACHUPM_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6570..7079
                     /gene="yihI"
                     /locus_tag="ACHUPM_002336"
     CDS             6570..7079
                     /gene="yihI"
                     /locus_tag="ACHUPM_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR0870093.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7268..8641
                     /gene="hemN"
                     /locus_tag="ACHUPM_002337"
     CDS             7268..8641
                     /gene="hemN"
                     /locus_tag="ACHUPM_002337"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR0870094.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8831..8941)
                     /locus_tag="ACHUPM_002338"
     CDS             complement(8831..8941)
                     /locus_tag="ACHUPM_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR0870095.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9053..10462)
                     /gene="glnG"
                     /locus_tag="ACHUPM_002339"
     CDS             complement(9053..10462)
                     /gene="glnG"
                     /locus_tag="ACHUPM_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR0870096.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10474..11523)
                     /gene="glnL"
                     /locus_tag="ACHUPM_002340"
     CDS             complement(10474..11523)
                     /gene="glnL"
                     /locus_tag="ACHUPM_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR0870097.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11697..13106)
                     /gene="glnA"
                     /locus_tag="ACHUPM_002341"
     CDS             complement(11697..13106)
                     /gene="glnA"
                     /locus_tag="ACHUPM_002341"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR0870098.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13479..15302
                     /gene="typA"
                     /locus_tag="ACHUPM_002342"
     CDS             13479..15302
                     /gene="typA"
                     /locus_tag="ACHUPM_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR0870099.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         4004 a         3829 c         3937 g         3567 t
ORIGIN      
        1 cagtagctga acaggaggga cagctgatag aaacagaagc cactggagca cctcaaaaac
       61 accatcatac actaaatcag taagttggca gcatcaccag cgagtcgaaa tttaatcaga
      121 ttaacattat caatcacggg gaagacgttg cccaaatcga tgctttcgtt gcaaaaacca
      181 tgctggatcg cgttaaagac aaagattata tcaatgtcaa tctgacctac gaacttgata
      241 agttaacaaa agggaatcag caactcggta gtggtgagtg gtcattgatt gctgaatcta
      301 ttgatccctc agcagtgcgc caatttatca tccagtataa cattgctatg cagaagcagc
      361 ttgctgtaca ccctgagtta gcaaacgatg aagttgctct gcaagaagtg aatgctgcat
      421 tgttcaaaga gtatttaccg ttattacaaa aaagtgagcc gaccattaaa caaccggtaa
      481 gatggaagaa cgcactcggc gaactaaatg ccaatctgga tatcagtatt gccgacccag
      541 ccaaatcttc atcatccaca aacaaagata tcaaattgct caattttgat gtgaagttac
      601 cgcttaatgt cgtcacagaa accgcaaaac agcttaattt atctgaagga atggatgcgg
      661 aaaaagcgca aaagcaggct gataaacaaa tcagcgggat gatgacattg ggtcagatgt
      721 ttcagttaat cacgattgac aacaataccg cctcgctgca gctgcgttat acaccgggta
      781 aagttgtgtt taacggacag gagatgagcg aagaagaatt tatgtctcgt gccggacgtt
      841 ttgttcatta aaatactcac tcgcctctgc tcattcagag gcgagtaaaa cttacttatt
      901 ctgacgctgt gatgacatga gagacgtcag aagccggtct ttttcctgcc acaatgagtt
      961 cagccattgc tgaaaatggc gcttaaagct tttgtcattg atgtagtcgc catgtaactc
     1021 atcggcaata ggctgtaaat ccacatgtac gacaatccgc gtcagtttac cgcttaacat
     1081 atcgaagaac ggctgacggt tattgtccgg ataacacagc gtaacattaa gcagtttatc
     1141 gaattgttta cccagtacat ttagcgccat cgcaatgcct gccgcctttg gtggcaacaa
     1201 gttttgaaaa gtggagtggg tttgctgatg tttttcttgc gtgaaacggg agccttcaac
     1261 gaaattgaca atggtggtgg gatgcaggcg aaacttttca caagaacgcc gagtggtttc
     1321 aacatcttta ccacgtcgct ccggatgacg taataaataa gcgcgggaat aacgcttcat
     1381 aaacggcata tccagcgccc agcacgccag gccaagaaat ggcacccagg ccagctgttg
     1441 cttgaggaaa tatttattca tcggaatgtg cttacgaaac agcacgcaca gtacgacaat
     1501 atctgcccaa ctacggtggt tacaaataag cagataccag ttcttcttac ttaacccttc
     1561 cagcccgtga acttcccatt gcaggtgtgg gttcagatgc agtaataccg ccagaccttc
     1621 acaccagcaa tacatcataa aatcacaaaa acgcgaaacc tttcgccaaa tgactggcac
     1681 aggtagcaat agttttacaa tcccggcaat gatgatcggg acggaacaaa aaatagtgac
     1741 caaaatggtc aatacgatac tcagcaaaag ggttatcgca gcgagtattc tcgtcataat
     1801 gaatttattc aaaaggttag ccatagacag tgcgctaaag aagcgcaagg cgcagatttt
     1861 agcagaaaac gacccaaata acggatgatc cttaaggaga aaaataatcc atatctatcc
     1921 acattagaaa aaatcccatt atctcaatta ttagggatgg ctttattttt aactgcatga
     1981 aaaacaaaga caaacatcat gctgtaaaaa gcatgataat aaattaaaag cgatgtaaat
     2041 aatttatgca caaagttatc cacatgacga tttgcgagcg atccagaaga tctacaaaag
     2101 attttcacga aaagcggtga aaaactcatg ttttcatcct gtctgtggca tcctttaccc
     2161 ataatctgat aaacaggcac ggacattatg gttcagatcc cccaaaatcc acttatcctt
     2221 gtagatggtt catcttatct ttatcgcgca tatcacgcgt ttcccccgct gactaacagc
     2281 gcaggcgagc cgaccggtgc gatgtatggt gtcctcaaca tgctgcgcag tctgatcatg
     2341 caatataaac cgacgcatgc agcggtggtc tttgacgcca agggaaaaac ctttcgtgat
     2401 gaactgtttg aacattacaa atcacatcgc ccgccaatgc cggacgatct gcgagcacaa
     2461 atcgaacccc tgcacgcgat ggttaaagcg atgggactgc cgctgctggc ggtttctggc
     2521 gtagaagcgg acgacgttat cggtactctg gcgcgcgaag ccgaaaaagc cgggcgtccg
     2581 gtgctgatca gcactggcga taaagatatg gcgcagctgg tgacgccaaa tattacgctt
     2641 atcaacacca tgacgaatac catcctcgga ccggaagagg tggtgaataa gtacggcgtg
     2701 ccgccagaac tgatcatcga tttcctggcg ctgatgggtg actcctccga taacattcct
     2761 ggcgtaccgg gcgtcggtga aaaaaccgcg caggcattgc tgcaaggtct tggcgggctg
     2821 gatacgctgt atgccgagcc agaaaaaatt gctgggttga gcttccgtgg cgcgaaaaca
     2881 atggcagcga agctcgagca aaacaaagaa gttgcttatc tctcatacca gctggcgacg
     2941 attaaaaccg acgttgaact ggagctgacc tgtgaacaac tggaagtgca gcaaccggca
     3001 gcggaagagt tgttggggct gttcaaaaag tatgagttca aacgctggac tgctgatgtc
     3061 gaagcgggca aatggttaca ggccaaaggg gcaaaaccag ccgcgaagcc gcaggaaacc
     3121 agtgttgcag acgaagcgcc agaagtgacg gcaacggtga tttcttatga caactacgtc
     3181 accatccttg atgaagaaac actgaaagcg tggattgcga agctggaaaa agcgccggta
     3241 tttgcatttg ataccgaaac cgacagcctt gataacatct ctgctaacct ggtcgggctt
     3301 tcttttgcta tcgagccagg cgtagcggca tatattccgg ttgctcatga ttatcttgat
     3361 gcgcccgatc aaatctctcg cgagcgtgca ctcgagttgc taaaaccgct gctggaagat
     3421 gaaaaggcgc tgaaggtcgg gcaaaacctg aaatacgatc gcggtattct ggcgaattac
     3481 ggcattgagc tgcgtgggat tgcgtttgat accatgctgg agtcctacat tctcaatagc
     3541 gttgccgggc gtcacgatat ggacagcctc gcggaacgtt ggttgaagca caaaactatc
     3601 acttttgaag agattgcggg taaaggcaaa aatcaactga cctttaacca gattgccctc
     3661 gaagaggccg gacgttacgc cgccgaagat gcggatgtca ccttgcagtt gcatctgaaa
     3721 atgtggccag atctgcaaaa acacaaaggg ccgttgaacg tcttcgagaa tatcgaaatg
     3781 ccgctggtgc cggtgctttc acgcattgaa cgtaacggtg tgaagatcga tccgaaagtg
     3841 ctgcacaatc attctgaaga gctcaccctt cgtctggctg agctggaaaa gaaagcgcat
     3901 gaaattgcag gtgaggaatt taacctttct tccaccaagc agttacaaac cattctgttt
     3961 gaaaaacagg gcattaaacc gctgaagaaa acgccgggtg gcgcgccgtc aacgtcggaa
     4021 gaggtactgg aagaactggc gctggactat ccgttgccaa aagtgattct ggagtatcgt
     4081 ggtctggcga agctgaaatc gacctacacc gacaagctgc cgctgatgat caacccgaaa
     4141 accgggcgtg tgcatacctc ttatcaccag gcagtaactg caacgggacg tttatcgtca
     4201 accgatccta acctgcaaaa cattccggtg cgtaacgaag aaggtcgtcg tatccgccag
     4261 gcgtttattg cgccagagga ttatgtgatt gtctcagcgg actactcgca gattgaactg
     4321 cgcattatgg cgcatctttc gcgtgacaaa ggcttgctga ccgcattcgc ggaaggaaaa
     4381 gatatccacc gggcaacggc ggcagaagtg tttggtttgc cactggaaac cgtcaccagc
     4441 gagcaacgcc gtagcgcgaa agcgatcaac tttggtctga tttatggcat gagtgctttc
     4501 ggtctggcgc ggcaattgaa cattccacgt aaagaagcgc agaagtacat ggacctttac
     4561 ttcgaacgct accctggcgt gctggagtat atggaacgca cccgtgctca ggcgaaagag
     4621 cagggctacg ttgaaacgct ggacggacgc cgtctgtatc tgccggatat caaatccagc
     4681 aatggtgctc gtcgtgcagc ggctgaacgt gcagccatta acgcgccaat gcagggaacc
     4741 gccgccgaca ttatcaaacg ggcgatgatt gccgttgatg cgtggttaca ggctgagcaa
     4801 ccgcgtgtac gtatgatcat gcaggtacac gatgaactgg tatttgaagt tcataaagat
     4861 gatgttgatg ccgtcgcgaa gcagattcat caactgatgg aaaactgtac ccgtctggat
     4921 gtgccgttgc tggtggaagt ggggagtggc gaaaactggg atcaggcgca ctaagattcg
     4981 cctgaacatg cccttttttc gtaagtaagc aacataagct gtcacgtttt gtgatggcta
     5041 ttagaaattc ctatgcaaca actgaaaaaa aattacaaaa agtgctttct gaactgaaca
     5101 aaaaagagta aagttagtcg cgtagggtac agaggtaaga tgttctatct ttcagacctt
     5161 ttacttcacg taatcggatt tggctgaata ttttagccgc cccagtcagt aatgactggg
     5221 gcgtttttta ttgggcgaaa gaaaagatcc gtaatgcctg atgcgctatg tttatcaggc
     5281 caacggtaga attgtaatct attgaattta cgggccggat acgccacatc cggcacaagc
     5341 attaaggcaa gaaaattatt cgccgtcctg cgtttcttct acaggctgca tctcgctaaa
     5401 ccaggtatcc agtttctgcc gcagcttgtc cacgccttgt ttcttcaacg aagaaaacgt
     5461 ttcaacctgc acatcaccgt taaacgccag tacagcttca cgcaccatat tcaattgcgc
     5521 tttacgtgcg ccgcttgcca gtttgtccgc tttggtcagc agcaccagaa cggcgatatt
     5581 gctgtctacc gcccactcaa tcatctgctg atccaaatct ttcagcggat ggcgaatatc
     5641 cattagcacc accagacctt gcaggctctg acgtttttcg aggtattcgc cgagcgcacg
     5701 ctgccatttg cgcttcatct cttccgggac ttccgcataa ccgtacccag gcaagtcaac
     5761 cagacgcttg ccgtcagcca cttcaaacag gttgataagc tgggtgcgcc ctggggtttt
     5821 tgaggtacga gccaggcttt tctggttagt cagcgtgttc agcgcgctgg atttacctgc
     5881 gttggaacgg cctgcaaaag ccacttcaat tccggtatcg gaaggtaggt ggcgaatatc
     5941 aggcgcactc atcacaaaat gcgtctgttg ataattcaaa ttagtcaaag cggtcgtctc
     6001 cgtcagtcaa agctttgtgg cgattatacc tgaaccataa taaaaggcgg atttttcggc
     6061 gtgagcgttg taagtaaaag ccatacgctt tgtgagacat tgccgatagt ctttatgcga
     6121 aatagcagag aaaattctgc gatgcatgtc aaataagcta tataaatcag tgaattgctt
     6181 ttatataaaa ctctgaaaat gtgcaaatac tgatggcggt tgattgtttg tttaaagcaa
     6241 aggcgtaaag tagcacccat agagcgagga cgctaacagg aacaatgact caggatgagg
     6301 gtcaggagcg ccaggaggcg aagacagagg attgtcagga agacaaacgt ccggagacgt
     6361 aattaaacgg aaatggaatc aacacggatt gttccggcta aaggaaaaac agggtgtgtt
     6421 ggcggcctgc aaggattgta agacccgtta agggttatga gtcaggaaaa aaggcgacag
     6481 agtaatctgt cgcctttttt ctttgcttgc tttctgttag attccgccgc aaatctatac
     6541 tgaataaaac ggctaaaaga cgaaccatta tgaaaccatc atcttcaaac tcacgcagca
     6601 aaggtcatgc aaaagcgcgt cgaaaaacac gcgaggagct ggatcaggaa gctcgtgatc
     6661 gcaagcgtca gaaaaaacgt cgtggtcatg cgccgggcag ccgcgcagcg ggcggaaaca
     6721 ccacgtcagg cagcaaaggc cagaacgcac caaaagatcc acgtattggc agtaaaaccc
     6781 ctattccatt gggcgtggct gaaaaagtca ccaaacagca caaaccgaag agtgagaaac
     6841 ctatgctttc accgcaggcg gagttggaat tactggaaac ggatgagcgt ctggatgcgc
     6901 tgctggaacg tctggaagca ggcgaaaccc tgagcgccga agagcaatcc tgggtggatg
     6961 ctaaactgga tcgtattgat gagttgatgc agaaactcgg cctctcttat gacgatgacg
     7021 aagaagagga agaagacgag aagcaagaag acatgatgcg tctgctgcgg ggtaactaac
     7081 ggattgccac cgtgggcctt cccgttctgc tgataaccct tccggttata tgttatctgg
     7141 tgtggttatt cgttaaacta cagcggttgt cgcggcgaca aaagtggctg cgcaaccggc
     7201 tgatgacccg aaacgggcat ccgccggtac gccgtagccg ccagagacgc catcggaagg
     7261 agtgagcatg tctgtacagc aaatcgactg ggatctggcc ctgatccaga aatataacta
     7321 ttccgggcca cgatacacct cgtacccgac cgcgctggag ttttcagaag acttcggcga
     7381 acaggcgttt ttacaagccg tggcgcgcta tcctgagcgt ccattatctc tctacgtaca
     7441 tatcccgttc tgccataagc tttgttactt ctgcggttgc aataagattg ttactcgcca
     7501 gcagcacaag gccgatcagt atctggacgc gctggagcaa gaaatcgtcc atcgtgcacc
     7561 gctgttcgcc gggcgtcacg tcagccaatt gcactggggc ggcggaacgc cgacgtatct
     7621 gaataaagcg caaatcagcc gcctgatgaa gctgctgcgc gaaaacttcc agttcaatgc
     7681 cgatgcggag atttcgatcg aagtcgatcc gcgggaaatc gaactggatg tactcgatca
     7741 tttacgcgcc gaaggcttta atcgcctgag catgggcgtg caggacttca acaaagaagt
     7801 gcaacgtctg gttaaccgcg agcaggatga agagttcatc tttgcactgc ttaaccatgc
     7861 gcgtgagatt ggttttacct ccaccaacat cgacctgatt tacggcctgc cgaaacagac
     7921 gccggagagt ttcgccttta ccctgaaacg tgtggcggag ctgaaccccg atcgtctgag
     7981 tgtctttaac tacgcgcatc tgccgaccat ttttgctgct cagcgcaaaa tcaaagatgc
     8041 tgacctgccg agtccgcagc aaaaactcga tatcctgcag gaaaccatcg ccttcctgac
     8101 gcaatcgggc tatcagttta tcggtatgga tcactttgcc cgtccggatg acgagctggc
     8161 ggtggcccag cgtgaaggcg tgctgcatcg taacttccag ggctacacca ctcagggcga
     8221 taccgatctg ctggggatgg gcgtttccgc catcagcatg attggcgact gctacgcgca
     8281 gaaccagaaa gagttgaagc agtactatca gcaagtggat gaacaaggca atgctctgtg
     8341 gcgtggtatt gcgctaacgc gtgatgactg tattcgccgc gatgtgatta agtcgctcat
     8401 ctgcaacttc cgtctggatt acgcccctat tgagaaacag tgggatttgc acttcgctga
     8461 ttactttgcg gaagatctca agctgctcgc cccgttagca aaagatgggc tggtggatgt
     8521 ggatgagaag ggaatacagg tgacggcgaa aggtcgcttg ctgatccgca acatttgcat
     8581 gtgctttgat acctatctgc gccagaaagc gcggatgcag cagttctcac gggtgattta
     8641 aatagtgaat ggcgcttcgt ttacaaagta gaaaacgaaa gcgccatcaa tgtgctgaga
     8701 acaagattgc ctgatgcgct acgcttatca ggcctacaaa acctgttgaa aatattgctt
     8761 ttgtaggccg gataaggcgt tcacgccgca tccggcatga acaaagagca cttcatcagc
     8821 aatctgaaac tcaactaaac agcccaatca ttgcggcaca cagcacggca gcaacggcag
     8881 tttgcggtgt gtggctgtca actgcgccac tcgataccag attaattatt gattccagca
     8941 taatgactct ccccgttttc cgggcaagat catactgaac ttatcggaac agtaaagcgt
     9001 aaaataccag caatttgcgc tcaataatca atctttacac acaagctgtg aatcactcca
     9061 tccccagctc ttttaactta cgcgtcaggg tgttgcggcc ccagccgagt agccgcgccg
     9121 cttcctgttt atgcccctgc gtatgtcgta acgcggtcgt cagtaacgtc cgctccagct
     9181 ctggctgcgc ttcggaaagc agattttgat gaccggaacg cagcgctctg tctgcccact
     9241 gcgcaagaag cgttgcccag ctgtccggtt gcatttgcga agtactctcc gcaaccgttg
     9301 attcaaacag ttcgccgggc aaatcctgaa tcaacacttc ctgcccggcg gccatcaccg
     9361 ttagccagcg gcaggtgttt tccagctggc gcacgttgcc tggccacgcc agacgcgtca
     9421 gagcagcttc ggtttccgga tgcagtaact tcgcttctac gcccagttcg cgcgcggcaa
     9481 cctgtaaaaa atggcgcgcc agacggggaa tatcttcccg acgttcgcgc agcggcggca
     9541 gatgaacgcg gataacgttc aggcggtgga acagatcctc acggaactta ccttcctgca
     9601 ctcgctgttc gagattctgg tgagtggcag cgataatacg cacatccact ttcaccggcg
     9661 catagccgcc aacgcggtaa aactgaccgt ctgccagcac gcgcagcaaa cgcgtctgca
     9721 catccagcgg catatcacca atttcgtcga ggaataatgt accgccatcg gcctgttcaa
     9781 aacgcccctg acgaatggta ttcgcgccag taaacgcgcc tttctcgtgg ccaaacagtt
     9841 ctgattcgat caaatctttt gggatagctg ccatattcag cgcgataaac ggcgctttgg
     9901 cgcgcggact gtggcgatgc agggcatgag cgaccagttc tttaccggtg ccggattcgc
     9961 cgttaatcag cacgctaata gaagaacgcg aaagccgacc gataatacgg aacacgtcct
    10021 gcatggctgg cgcttcgccg atgatatcgg tcgttgggcc gttaagctga atattacgcg
    10081 gctgctgctg ttcctggtaa tgactgatag cgcgctcaac cagcgccact gcttcgtcga
    10141 tatcaaacgg tttgggcaga taatcaaacg ccccttgttg ataggcgctg acggcagcat
    10201 ccagatcgga atgtgcggtc ataatgatga ccggaagcat tggatggcgc tgtttaatct
    10261 gcttgagcag cgccagcccg tccattcccg gcatacggat atctgaaagc agcacatccg
    10321 gcgttttgct cgccagcgcc tccagtactt ccgcgccgtt ctcaaatgtc gtacaggtta
    10381 aacccgctcc agcgagcgca cgttcaagca cccaacggat ggaactatcg tcatcgacta
    10441 cccagactat ccctcgttgc ataaacgtca cctttatttc ctgataggca ggtaaaccga
    10501 gaactcggta tgacctggcc aactggtaaa ttcaattttg cctgaatgct gatcaatcaa
    10561 attacgggcg atggataagc caagcccggt gccaccttcg cggccgctga ccatcgggta
    10621 aaacagcgta tcctgcaaat gaggcggaat gcccggcccg ttatcttcca catcaatccg
    10681 cgccgccagc cggtagcgct cgccgtgtaa ggtcagttga aacgcggtgc gggtacgcag
    10741 aatgatttca ccgccttccg gccccagcgc ctgtagcgca ttgcgcacaa tattcagcaa
    10801 gacctgttca atttgatccg ggtcgtgcgc cagttccggc aggctggggt cgtaatcacg
    10861 aatcaaccgc acgttgtccg gcagttccat cgacaccagc gtcaccacgc gttcagctac
    10921 tttgtgaata ctttcggtaa cgcgcgtacc gggcagctgc ggccccaaca gacggtcgac
    10981 cagatttcgc agccggtccg cctgttcgat aatcactttg gtatattcga gtagtgatgg
    11041 gtcaggtaac gctttgctga gcagctgcgc cgcgccacgt aaacctccaa gcggattttt
    11101 aatctcatgt gccaggccgc gcactaaatc acgggcagca acctgctggg cgtgctgtag
    11161 ctgttcctga cttaagcggc gctggttatc catcggagcc atctccagca ggatcatgcc
    11221 gtccggcata cgctgggccg tcacagaaag gatatgcgag cgcccgtcga tgaccagcgt
    11281 cacttcgtta tcggtaaaac cttgccccgc ctccagactt tcttgcatca gctcgatatt
    11341 taatgagaag tagctcaaca gttccggtaa cggtgtacca aacaatttgc gggagctttg
    11401 ggcgagcagt tgttgcgcgg cggggttggc gtaatggatc gccagattgt catcaattaa
    11461 caaaatactg ttaatcagcg agttgaggat ctgcccagca tcgggctgcg tgcctgttgc
    11521 cataaagcag tctcctgaac aggttgcacc attttagtgc attatagctt tttacggata
    11581 aaaagcgcga agcatcagag aattgacgga gaaaaaagcc catgcagaga tgggctgaaa
    11641 gtttccacgg caactaaatc ccggcgttgt tgcgccgggt agtacaactt caactattag
    11701 acgctgtagt acagctcaaa ctctaccgga tgcggagtca tacgcacgcg gtcatcttct
    11761 tcgcgacgta gagcgatgta cgcatcaatt gcttcgtcag tgaacacgcc accggctttc
    11821 aggaactcgc ggtccagatc cagttcgttc agtgcttctt ccagagagcc tgcaacctgt
    11881 gggatctctt tcgcttcttc tggcggcagg tcatacaggt ttttgtccat ggcttcgccc
    11941 ggatggatct tgttcttgat accatcaaga ccggccatca gcagggcagc aaagcacagg
    12001 tacgggttag ctgccggatc cgggaaacgt acttcgatac gacgtgcttt cggagaagaa
    12061 accaccggaa tacggataga cgcagaacgg ttacgcgcag agtaagccag cattaccggt
    12121 gcttcatagc ccgggaccag acgcttataa gagttggtgg tcgggtttgc cagggcgtta
    12181 atcgctttag cgtgtttgat tacgccgcca atgtagtaca gcgcctgctc agacagacct
    12241 gcgtatttgt cgcctgcgaa caggttaacg ccgtttttag acagagacat gtggcagtgc
    12301 ataccggagc cgttatcacc gaacatcggt tttggcataa aggtcgcggt tttaccgaag
    12361 cggtgcgcta cgttgtgcac aacatatttg tagatctgaa tttcgtcagc ttttttggtc
    12421 atagtattga agcgggtagc cacttcgttc tgaccagcag tcgctacttc gtggtgatgg
    12481 gcttcaacca ccagacccat ctgttccatc accagacaca tttcagaacg aatatcctga
    12541 gccgagtcta ccggtggaac cgggaagtaa ccgcctttca ctgccggacg gtgacctttg
    12601 ttaccacctt cgtattgggt ggaggagttc catgcgcctt cgatatcgtc gatagcaacg
    12661 tgggaaccgg agatagatga tccgaaacgg atgtcatcga acaggaagaa ttcaggttct
    12721 ggcccgaaca gtacggtgtc ggcaatgcca gtggaacgca ggtaatcttc ggcgcgcttc
    12781 gcaatggagc gcgggtcacg gtcatagcct tgcagggtgc caggttcaag gatgtcgcaa
    12841 cggataatca gggtggagtc ggcgaagaac gggtcaatca ctgcggtgga tgcgtctggc
    12901 atcagcacca tgtcggactc gttaatgcct ttccagccgc caatcgagga gccgtcaaac
    12961 attttgcctt cttcgaagaa ttcagcattc acctgatgag cagggatagt gacgtgctgt
    13021 tctttacctt tagtatcggt gaagcgcaaa tcaacaaact tcacttcgtg ctcgttcagc
    13081 atcgtcagta cgtgttcagc ggacatactt taactctcct ggattggtca tggtcgtcgt
    13141 ggtaacgaaa tctgcaatta ttttggccgt gtcgccgtaa aaaagataaa gcgaaatctg
    13201 tgccaacttt taaattgccc ctaaaaggcg ttatcatgcg caccatcgtg caaaagggct
    13261 gcaccacgat gtgaatgttg caccaatata gtgcttcaat ggaaacatta agcaccatgt
    13321 tggtgcaatg acctttggat aacccttttt atgctccgtg aaagcgatca caaagggact
    13381 ctgcaatact tgtttgcgga ggatgtttgt gatcctgttt tgtagtgcga ttaatccgtg
    13441 tacaataacg cgctatttct aatgcctgag gcaaagttgt gatcgaaaaa ttgcgtaata
    13501 tcgccatcat cgcgcacgta gaccatggta aaaccaccct ggtagacaag ctgctccaac
    13561 aatccggtac gttcgactct cgtgccgaaa cccaagagcg cgtgatggac tccaacgatt
    13621 tggagaaaga gcgtgggatt accatcctcg cgaaaaacac cgctatcaaa tggaatgatt
    13681 accgtatcaa catcgttgat accccggggc acgccgactt cggtggtgaa gttgaacgtg
    13741 taatgtccat ggtagactca gtgctgctgg tggttgacgc atttgacggc ccgatgccgc
    13801 aaacgcgctt cgtaaccaaa aaagcgtttg cttacggcct gaagccgatt gttgttatca
    13861 acaaagttga ccgccctggc gcgcgtcctg attgggttgt ggatcaggta ttcgatctgt
    13921 tcgttaacct cgacgcgacc gacgagcagc tggacttccc gatcgtttac gcttctgcgc
    13981 tgaacggtat cgcgggtctg gaccacgaag atatggcgga agacatgacc ccgctgtacc
    14041 aggcgattgt tgaccacgtt cctgcgccgg acgttgacct tgacggtccg ttccagatgc
    14101 agatttctca gctcgattac aacagctatg ttggcgttat cggcattggc cgcatcaagc
    14161 gcggtaaagt gaagccgaac cagcaggtca ctatcatcga tagcgaaggc aaaacccgca
    14221 acgcgaaagt cggtaaagtg ctgggccacc tcggtctgga acgtatcgaa accgatctgg
    14281 cggaagctgg cgatatcgtg gcgatcacgg gccttggcga actgaacatt tctgacaccg
    14341 tttgcgacac gcaaaacgtt gaagcgctgc cggcactctc cgttgatgag ccgaccgttt
    14401 ctatgttctt ctgcgttaac acctcgccgt tctgcggtaa agaaggtaag ttcgtaacgt
    14461 ctcgtcagat cctggatcgt ctgaacaaag aactggtaca caacgttgcg ctgcgcgtag
    14521 aagaaaccga agacgccgat gcgttccgcg tttctggtcg tggcgaactg cacctgtctg
    14581 ttctgatcga aaacatgcgt cgtgaaggtt tcgaactggc ggtatcccgt ccaaaagtaa
    14641 tcttccgtga aatcgacggt cgtaaacaag agccgtatga aaacgtgact ctggacgttg
    14701 aagaacagca tcagggttct gtaatgcagg cgctgggcga acgtaaaggc gacctgaaaa
    14761 acatgaatcc agacggtaaa ggccgcgtac gtctcgacta cgtgatccca agccgtggtc
    14821 tgattggctt ccgttctgag ttcatgacca tgacttccgg tactggtctg ctgtactcca
    14881 ccttcagcca ctacgacgac gtacgtccgg gtgaagtggg tcagcgtcag aacggcgtac
    14941 tgatctctaa cggtcagggt aaagcggtcg cgttcgcact gttcggtctg caggaccgcg
    15001 gtaagctgtt cctcggtcac ggtgcagaag tttacgaagg tcagattatc ggtattcaca
    15061 gccgctctaa cgacctgact gtaaactgcc tgaccggtaa gaaactgacc aacatgcgtg
    15121 cttccggtac tgacgaagct gttgttctgg ttccgcctat ccgcatgact ctggaacaag
    15181 ctctggagtt catcgatgat gacgaactgg tagaagtgac tccgacctct atccgtattc
    15241 gtaaacgtca cctgacggaa aacgatcgtc gtcgcgccaa ccgcgcaccg aaagacgatt
    15301 aatttcgttt tttagttata aaaaacctgc catcgct
//
LOCUS       DAZPAC010000090        15328 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZPAC010000090 DAZPAC010000000
VERSION     DAZPAC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15328
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.090"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(36..119)
                     /locus_tag="ACHUPM_002343"
                     /pseudo
     CDS             complement(36..119)
                     /locus_tag="ACHUPM_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
     gene            complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="ACHUPM_002344"
     CDS             complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="ACHUPM_002344"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR0870100.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="ACHUPM_002345"
     CDS             complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="ACHUPM_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFR0870101.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="ACHUPM_002346"
     CDS             complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="ACHUPM_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR0870102.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5772..6269
                     /gene="msrC"
                     /locus_tag="ACHUPM_002347"
     CDS             5772..6269
                     /gene="msrC"
                     /locus_tag="ACHUPM_002347"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR0870103.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6366..7064
                     /gene="proQ"
                     /locus_tag="ACHUPM_002348"
     CDS             6366..7064
                     /gene="proQ"
                     /locus_tag="ACHUPM_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR0870104.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7084..9132
                     /gene="prc"
                     /locus_tag="ACHUPM_002349"
     CDS             7084..9132
                     /gene="prc"
                     /locus_tag="ACHUPM_002349"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR0870105.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9324..10205
                     /gene="htpX"
                     /locus_tag="ACHUPM_002350"
     CDS             9324..10205
                     /gene="htpX"
                     /locus_tag="ACHUPM_002350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR0870106.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10251..11314)
                     /locus_tag="ACHUPM_002351"
                     /pseudo
     CDS             complement(10251..11314)
                     /locus_tag="ACHUPM_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11491..12282
                     /gene="kdgR"
                     /locus_tag="ACHUPM_002352"
     CDS             11491..12282
                     /gene="kdgR"
                     /locus_tag="ACHUPM_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR0870107.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12425..12664)
                     /locus_tag="ACHUPM_002353"
     CDS             complement(12425..12664)
                     /locus_tag="ACHUPM_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR0870108.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12823..12966
                     /gene="mgrB"
                     /locus_tag="ACHUPM_002354"
     CDS             12823..12966
                     /gene="mgrB"
                     /locus_tag="ACHUPM_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR0870109.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13041..13328
                     /locus_tag="ACHUPM_002355"
     CDS             13041..13328
                     /locus_tag="ACHUPM_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR0870110.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13998..14141
                     /locus_tag="ACHUPM_002356"
     CDS             13998..14141
                     /locus_tag="ACHUPM_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR0870111.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14154..14363
                     /gene="cspE"
                     /locus_tag="ACHUPM_002357"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14154..14363
                     /gene="cspE"
                     /locus_tag="ACHUPM_002357"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0870112.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14529..>15328
                     /gene="rlmA"
                     /locus_tag="ACHUPM_002358"
     CDS             14529..>15328
                     /gene="rlmA"
                     /locus_tag="ACHUPM_002358"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR0870113.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQR"
BASE COUNT         3987 a         3709 c         3912 g         3720 t
ORIGIN      
        1 cataaaacac tatcaataag ttggagtcat tacctggtta acgttcgatc acccggcgat
       61 tcaccgtgta attcgccgtc gtcgatttca aaagcgccta tgcgcacact ggttttcatc
      121 ttcgtgctct ccatttagct gccggttgtg gtacgaccag tccgatcgca ccatttttca
      181 gcctagttga ggcagccgaa tgtgccagtc aaaaagtgcg ctttttttgt gcgctgtcag
      241 gcgttaccgg taaaaagttt cccatcgcgc accagttcac gcggatagct gtttttcaat
      301 cgcgaaccaa tccgtttggc taaaccaatc ggttgatgct ggaaagtaac caacacgtca
      361 tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc ctccgcttcc
      421 tgcggtgtca gttcaaaagc gttcacattg tcgggggtgg caagggcaat aactgcttca
      481 tgctgccagc gataaccttt gttgtgcgtt tcggcaagtt taatccccaa gcgagaaaat
      541 cggactttac cgatcagggc ttcaatgccc accgggaaca accacagttc tttgtcacgc
      601 tgccagaggc gcaggttttc atcccagttt aagccaacac ctgcagccgc ctgacgaatt
      661 tgtccagctt ctcgatcttt cactgggctg aacggaaaat taccgacttt gtatttgggg
      721 gcgggtaagg cgggaatcgc ctgagtttta cgcagacgag caacgaagaa gccttcgcag
      781 tcgtaaattt gtgggaaaac atgcaaaaag ccttcctcgg tcagcgcctt gtttgcacca
      841 gggaagagat caccaagcgg taaaaactct actgcgtcgg ggtaagtctc tttcagccac
      901 aggcaaacgg cttcgttttc ttcctggttt aaggtacagg tcgagtaaac cagcgtaccg
      961 ccagggcgta atgcatgaaa ggcgctgtcg atcagttccc gttgggtagc tgcgatttcc
     1021 tgattgcttt ctggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg
     1081 ccggagcagg gagcgtccag caaaatggca tcaaacattt ctggcactgc cgcaccaaac
     1141 acgcggccat caaaatgtgt gagcgcaaca ttactgatac cacagcggct gatattggca
     1201 tgtaacactt ttacccgact ggcggaaaac tcattggcaa gaattgcccc ttcgttattc
     1261 atccgcgcgg aaatttgcgt cgttttggag ccaggcgcgg cagcgacatc catcacccgc
     1321 tgtggtgcat taccgtcagc aaacaaggcg gcgacgggca acattgaact ggcttcctga
     1381 atataaaaca ggccacttaa atgctcggcg gtactaccca atggcaatgc atcttcattg
     1441 tcgcgttcaa tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa
     1501 ggagcggtta attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg
     1561 cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa aagagagcgt cgaaggcatc
     1621 gcttcgcgca tttgcgtcag aaaggcgtcc gggaaataaa cggtgtgttg ggccacgagc
     1681 atataccact ggcaaaaaaa acaggcgcgc agtttagcat aaacgctccg gcgcaggcac
     1741 gccggagcag tgggcattat ttgggaagcg cagtacccca ttcacgccac tctttcggtt
     1801 cactttcctg caacaggaag tgcttgcctt cctgggcttt cggtgccagt ggcgtacccg
     1861 gaggcgtggc gaaggcgatg ccgccacgga taaactggtt aaaggtgccg gttttcacta
     1921 cgccgcccgt cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt
     1981 tacgcaccag gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca
     2041 atgtccccag cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg
     2101 taccaatacc taacgaaccg gcttccggtg cttcgacaat aatgcttaag ccatccaggt
     2161 aacgcgaatc agtaatagta gcttcttgca actcaaagtc acggcgagga ttaccccgac
     2221 ctggttcgac attgatatac ggctggagga tagtatctag atgctcaacg cccgcggctg
     2281 aaatttgcgg tgtgaccacc gagaagcgcg taccgccgcg ggcgaaggtc tggacatact
     2341 ccggatagag caccgccttt gcctgtactt cattgcgcgc ggtaatcaga tccagcgtct
     2401 ggatttgccc gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg
     2461 catcgaaagc gtgaagcgta atctgcccac caaccgcacg ggccgctgtt tcggaagcat
     2521 acagaattcg tttgtcgcct ttacgctgac tggcgctggc accgctgagg ttatcgaagc
     2581 taatggctcc ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat
     2641 tcagctgaac tttcgccccg ccttctgccc agaacacgct attgctggtc agaaggttgc
     2701 gatactccgg cttaatatgc agatcgatat caaacgcgtt agctcgcgga cgcacggtaa
     2761 tcacttcacc aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca
     2821 gcgtctctgc actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg
     2881 ctttttccag attggcatac agtggatagc tggctttcat ctcaccttta tcgcctggca
     2941 gaatacgtat cccgccgtta atccattctg aggcgctggc accaagaaac tcaacgccat
     3001 ccagccccac cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca
     3061 gttcgcgatg ctgaggctcg atggcgacgg taaaggtaac gcctttgctg gtgagtttac
     3121 gatcaataac ctggcctacc tgcacgccgt gaagaatgag cggctgacca gcatcaatac
     3181 cgtaactttc cggcgcggtc agggtcagcg tcagaacatc aggttcatgc agcagtgctt
     3241 tttcgcctgg cacaacaacg aactctttgc gtggctcgcc atcgccgggt accaactcga
     3301 aggttttgcc agtcagcagg gcgctgagat tggcatcgct aagggataat ttcggattaa
     3361 gtaattcgat gcgggtattt tcacgaagca gggtaacgac gctgggatta actgtcattt
     3421 ccccagtgac tttaccacca ggatttaaat ccagtttagt tagctgtccg acttccagtc
     3481 cctgatacat taatggcgtc gagtcggcgg ttaatccggc cccactcggc agttccagtt
     3541 tgattattac gccacgctgg ctatgggcga ggtcttcata cagaccaaag gtatcttccg
     3601 cctcggcagg tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg
     3661 ccagactttc cagtttcacc ttcgcaccac tgatactgac gttggcatca acgccggaaa
     3721 cgttccagaa acggctacct tttttcacta ggtcggtaaa acgccgctcg atcaggacat
     3781 caatcaccac gccttgcttg ttgggattga tggcatagtc gtagactttt cccacaggga
     3841 tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc gagatcgggg gcttgcaggt
     3901 ggatcatcag atcgccattg tccagtcgat atttcggttg ggtatcgagt gcgacaaagt
     3961 gatcctgctc tttaccttta cctggcatca tgccgatata gttaccaccg acgagggcgt
     4021 ccagcccgga gacacctgcc aacgatgctt ttggcgtcac cagccagaac tgagtctctt
     4081 cgcgcagcgc atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat
     4141 cgtcgctgag gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg
     4201 tacggcccgg gacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt
     4261 cctgataact gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc
     4321 agaaaggtga gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt
     4381 cctgactcat gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc
     4441 aaaattacgg ctgcgccaaa ataaaacgca gccggtccca tagtaaaagc gaggatctga
     4501 tcgcgattaa tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt
     4561 ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta
     4621 aaatgaatgc tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata
     4681 ctggcgataa acacgattcc ggcgaccgca atgttgctac ttgccagcga cataatcccg
     4741 gaaagaatcg tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg
     4801 ttggctggta acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta
     4861 tgacgacgac gcaggcgtag cgggatatgg caacgcgggc agcgaccacg ctgatcagga
     4921 tagccggtaa aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg
     4981 cgctgcggat aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc
     5041 gttaaaatca ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa
     5101 tcctgtacct ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac
     5161 tctttaagac gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca
     5221 aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag
     5281 accatcgccc ccgttatcgt atcgccctgt ttggtcattt gccagatgcc ttgcatgacg
     5341 ttggcgtcga tacgaatacc taacagccag atatgcaaca gcggttcgcc ccaggcaaac
     5401 ggcatcagca acagcatggt gaaagccatt gccgccaggc gcgttagcga ccagtcgcgc
     5461 ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tctgatgaga atttatctcg
     5521 ggcaggctaa acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc
     5581 tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc
     5641 atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt
     5701 gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta agacaaacaa
     5761 gtgattatat aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc
     5821 tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg
     5881 agcgtctcac tgacgtaaac tgggcaggtt tttatttgct tgaggacgat acactggtac
     5941 tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg
     6001 gcactgcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc
     6061 atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc
     6121 agattattgg tgttcttgac atcgatagta ccgtcttcgg tcgctttaca gacgaggacg
     6181 agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca
     6241 aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg
     6301 tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa
     6361 atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg
     6421 aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa atcggtattt
     6481 ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat
     6541 ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa
     6601 cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg
     6661 ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag
     6721 cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcactg cgccgcgaac
     6781 gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc
     6841 aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc
     6901 cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa
     6961 acgcgatgga tgccaccgta ttagaaatca ccaaagatgg cgtccgcgtc cagctgaatt
     7021 cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca
     7081 ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag
     7141 accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg
     7201 cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag
     7261 ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat
     7321 tacagccaca acgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag
     7381 ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg
     7441 caaaagcgcc gttttgagcg ttaccagtac gctttgtcgg tactggaaaa gccgatggat
     7501 ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag
     7561 gctgagttga acgcgctgtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg
     7621 gcaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt
     7681 cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg
     7741 cgtgaaatcg acccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact
     7801 gaaatgagtt tgtcgctgga aggtattggc gcagtgctgc aaatggatga tgactacacc
     7861 gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt
     7921 gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt
     7981 cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt
     8041 ttacctgctg gtaaagggac caagacccgc actgtaacat tgacccgtga acgtattcgt
     8101 ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggcgtg
     8161 ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa
     8221 ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg
     8281 ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc
     8341 cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa
     8401 ggcccgctgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg
     8461 gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc
     8521 gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca
     8581 gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg
     8641 caacgtaaag gcgtaacgcc agacatcatc atgccgacgg gtaatgaaga aacggaaacg
     8701 ggtgagaaat tcgaagataa cgcgctgccg tgggatagca ttgatgccgc gacttatgtg
     8761 aaatcaggcg atttaacggc ctttgggccg gagctgctga aggaacataa tgcgcgtatc
     8821 gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgagg
     8881 gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat
     8941 gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg
     9001 aagaagctgg atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg
     9061 gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaacccgct
     9121 cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag
     9181 atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac ttacgcacta
     9241 tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt
     9301 taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg
     9361 ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc
     9421 aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga
     9481 tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg
     9541 aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggca gggatcgcta
     9601 tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc
     9661 gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag
     9721 ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga
     9781 cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc
     9841 ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc
     9901 tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta
     9961 tcaccatgtg gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg
    10021 ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag
    10081 aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt
    10141 tcatgactca cccgccgctg gataaacgaa ttgaagctct gcgtacgggt gaatacctga
    10201 agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccttg
    10261 gatcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc
    10321 gcagccatca gcgaaacgtg cgtaccatta tcaccgaact gatttagcat cagcgccacc
    10381 agtgccgcgc cgctactctg acccagtaga cgagccgttc ctaacatgcc actggctcca
    10441 ccgctacgtt cgcgaggagc ggaggtaata atggtgtggt tattgggtga ctggaacaag
    10501 ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg
    10561 ggcagcagaa ccaggggaaa aagccccgca gccatgatga acaaccctag agcacccagc
    10621 aatcctgcat gtacgcgttc aatcaaatag cctgccaacg gagccatcac catcgttgct
    10681 aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc
    10741 aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg
    10801 caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga
    10861 agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca
    10921 gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg
    10981 aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa
    11041 ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg
    11101 attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg
    11161 gcagcagaag agacggcaac aatatggcga ttgttggcag ggcgacgttg gcgattgcgc
    11221 cgtcaaggac agccatcgaa atgccaatca caatggttaa tatcgcaccg tatcgctggg
    11281 gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt tggtgaataa
    11341 tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa
    11401 ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa cagttctgat
    11461 ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga taaacagcct
    11521 gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg tgaagagcgc
    11581 gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc
    11641 tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc ggagaaatat
    11701 tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt
    11761 cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac tatccacctc
    11821 ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat gtacaatttg
    11881 cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta
    11941 ctgctggcat ggcgcgatct cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa
    12001 cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct ggaccaggtg
    12061 cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg atgcattgcg
    12121 gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc cttcccgacg
    12181 ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc
    12241 aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta agaacctgtc
    12301 ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg aaggtgcgtt
    12361 atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg gttgtttatt
    12421 acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa tctgttatcc
    12481 ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg gcagtcagat
    12541 aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa acgccatatc
    12601 cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga atgatgaatc
    12661 gcattacaac ctcttctctt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    12721 aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt taactaacgc
    12781 atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg
    12841 gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    12901 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    12961 tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt agaatagcgc
    13021 ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt
    13081 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttatcaatc
    13141 gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    13201 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    13261 aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc ctggtcgccg
    13321 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    13381 tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa
    13441 taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt tcctcattaa
    13501 tttattagga tgtttacatc ggatttgtga ttaagcgtgg tattatttat tagcgaaacg
    13561 tttctctctt gatttttttg ctcattcatc aatttttctt attttaaatt ttcaatccct
    13621 ttggggattg acttctcttt agggtaattg atagccgtta accgactgtt ttatgagaaa
    13681 aagtgatata actttttatt cattgcatag caaaaaatgt gatattgcac gcactatgta
    13741 ataacttctc ccactggcct ggaacaactg aacttattga actatgttag aaaatacgcc
    13801 agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg cgcgtattcc
    13861 gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac
    13921 atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt cgggttaaac
    13981 gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt aaacacgttg
    14041 acgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt aatgtgtcag
    14101 ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt caaatggcaa
    14161 agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc attactccgg
    14221 ctgatggcag caaagacgtg ttcgtacact tctccgctat ccagggtaat ggcttcaaaa
    14281 ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa ggtccggcag
    14341 ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat acgcttcaat
    14401 ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt cgctcctggc
    14461 gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag tatcctgccg
    14521 gagttgtcat gtctttttcc tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca
    14581 gctatatctg tccccagcga catcagtttg atatggcgaa agaagggtat gtcaatctgc
    14641 tgtccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac
    14701 gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt gtcgcccaac
    14761 tgagggaacg gcttgatgat aaggccacgg cggtgctgga tattggctgt ggtgaagggt
    14821 attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggt ctggatgttt
    14881 cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactttt tgtgtcgctt
    14941 ccagccaccg tttaccgttt tccgatacca gtatggacgc cataatacgt atttacgcgc
    15001 cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc attactgcca
    15061 cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa gtacatcttc
    15121 atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg gagttgtgtt
    15181 atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg ccgtttacct
    15241 ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc gactgccaga
    15301 cggactttaa tattcacctc tggcagcg
//
LOCUS       DAZPAC010000091        15209 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZPAC010000091 DAZPAC010000000
VERSION     DAZPAC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15209
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.091"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="ACHUPM_002359"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="ACHUPM_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR0870114.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="ACHUPM_002360"
     CDS             433..621
                     /locus_tag="ACHUPM_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR0870115.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="ACHUPM_002361"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="ACHUPM_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR0870116.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="ACHUPM_002362"
     CDS             816..989
                     /locus_tag="ACHUPM_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR0870117.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="ACHUPM_002363"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="ACHUPM_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHUPM_002364"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHUPM_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFR0870118.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="ACHUPM_002365"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="ACHUPM_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR0870119.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHUPM_002366"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHUPM_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR0870120.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="ACHUPM_002367"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="ACHUPM_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR0870121.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="ACHUPM_002368"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="ACHUPM_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10407..10952
                     /gene="torD"
                     /locus_tag="ACHUPM_002369"
     CDS             10407..10952
                     /gene="torD"
                     /locus_tag="ACHUPM_002369"
                     /inference="COORDINATES: protein motif:HMM:NF003442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR0870122.1"
                     /translation="MFSRELDDEQLTQIASAQMAEWFSLLKSEPPLTAAVNALENRIA
                     ALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGMETSGNFN
                     ESADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSF
                     GFYAALSQLLLVLVECDHQNR"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHUPM_002370"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHUPM_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR0870123.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHUPM_002371"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHUPM_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR0870124.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12531..13677
                     /locus_tag="ACHUPM_002372"
                     /pseudo
     CDS             12531..13677
                     /locus_tag="ACHUPM_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13971..>15209
                     /gene="agp"
                     /locus_tag="ACHUPM_002373"
     CDS             13971..>15209
                     /gene="agp"
                     /locus_tag="ACHUPM_002373"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR0870125.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
BASE COUNT         3830 a         3739 c         3901 g         3739 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt aaattttata gacgctatat atgggtagta
    12541 atatacatgg aattagttgc actgcaaata attatttgaa acaggcctgg aacgatataa
    12601 aaaatgagta cgaaaaaaat caaacatatt caatcacgct ttttgaaaac acactggtgt
    12661 gttttatgcg gttatacaat gaactcagac gtaaagtaaa tgaagaggat actccatgtc
    12721 tggaatgtga atcactagaa aaagaatttg aggaaatgca gaatgataat gatctatcat
    12781 tatttatgag aacattgcgt actaatgata cacaaattta ttcaggggtt tcaggaggta
    12841 ttacatatac tatacaatat gttcaaaatg ttgatattgt tagagtgtcc ttgccgggca
    12901 gaggttcaga gtctatcaca gattttaaag gttattattg gtatggcttt atggaatata
    12961 ttgaaaacat tactgcgtgt gatgacattt tttctgagta ttgtttagat gatgaaaata
    13021 tgagtatcca gccagaacag ataaatacgc cgggaatatc tgatttagat actggtttta
    13081 aaagaacatg agtattcatg ggcgctgggc ggccataaga cgcaaaagca agtatacgaa
    13141 gagtttatta aaattgttga taaacaaacg atcgaggctc ttgttgatac ggcattctat
    13201 aatctcaggg aagatgtaaa gacgttattt ggcgttaatc tacaatctga caacaaaatt
    13261 caggggcagg gtggtttttt atcatggagt tttttgtctt ttaaaaaaga atttattgat
    13321 agttgcttga atgacaaaaa atgcatactg catttacccg aatttatatt taatgataac
    13381 aaggcacgcc ttgttttaga caccgaccca gagcagaagg ttaatgaggt gaaaaatttc
    13441 ctcactgctc tttcagatag catttgctca ttatttattg tttacagcaa tgtggcatca
    13501 atctccttag ggaatgaatc cttttcaaca gatgacgacc ttgagtatgg gtatttaata
    13561 aacactggca atcattatga cgtttacctc cctcctgaac tttttgctca ggcttacgag
    13621 ttaaacaata aggaaaggaa tgcgcaaatt gattttttaa ctcgttatgc aatttaatga
    13681 caaaggcatg tgctaaaaac cattgttatt agcctcactt ctcttattat tggtactatt
    13741 gcctctgtat tagtaacgcc gctgatattc tgtttatcca caggtgcaat tatcagcggc
    13801 gtacgcgagg caggggctaa tcaggcatag tttgcgtcaa accttgcctg tttttgaaga
    13861 tgtatataga aaaacaggcg ttcaacaagc cattttgcga acctgttccc ggaaaaaagt
    13921 catatttctg tcacactctt taatgattga taacaaaaga ggtgccagga atgaacaaaa
    13981 cgctaatcgc cgcaactgtg gcagggatag ttttactcgc ttcaaatgct caggcacaaa
    14041 ccgtaccgga aggctatcag ctacagcaag tgctcatgat gagccgccat aacttacgtg
    14101 cgccgctggc gaacaatggc agtgtgctgg agcagtcgac gccgaataaa tggccagaat
    14161 gggacgtccc cggtgggcaa ctcaccacca aaggtggcgt gctcgaagtg tatatgggcc
    14221 attacatgcg tgaatggctg gcagagcagg ggatggtgaa atcgggggaa tgcccgccgc
    14281 cggacaccgt ttatgcctat gccaatagtc tgcaacgtac cgttgcgacc gcacagttct
    14341 ttattaccgg cgcattcccg gggtgtgata ttcctgtgca tcaccaggaa aaaatgggca
    14401 ctatggaccc aacctttaac ccggtgatca ccgatgattc cgccgcattc agtgaacagg
    14461 cggtggcggc aatggagaaa gagctcagca aactccagct taccgacagc taccagctac
    14521 tggaaaaaat cgttaactat aaagattccc ctgcctgtaa agagaaacaa cagtgttcgc
    14581 tggtggatgg caaaaatacc tttagcgcca agtatcaaca agaaccaggt gtttccgggc
    14641 cgctgaaagt cggcaactcg ctggtagatg cgtttacttt gcaatattac gaaggttttc
    14701 cgatggatca ggtggcctgg ggagaaatca aatctgacca gcagtggaag gtgttgtcga
    14761 agcttaaaaa cggctaccag gacagcctgt ttacctcacc ggaagtggcg cgcaatgttg
    14821 cgaaaccgct ggtcagttat atcgacaaag ctctggtcac cgatcgcacc agcgcaccga
    14881 aaattacagt gttggttggg cacgactcca acattgcctc tctgttaacg gcgctggatt
    14941 tcaaaccgta tcagttgcat gaccagaacg aacgcacgcc gattggcggc aaaatcgttt
    15001 tccagcgttg gcatgacagc aaagccaatc gcgatttgat gaaaattgaa tatgtgtatc
    15061 agagtgcgga acagttacgt aatgccgatg cgttaaccct gcaggcacct gctcagcgtg
    15121 tgacgctgga attaagcggt tgcccgatag atgccaacgg tttctgcccg atggataaat
    15181 ttgatagcgt gttgaatgaa gcggtgaaa
//
LOCUS       DAZPAC010000092        15187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZPAC010000092 DAZPAC010000000
VERSION     DAZPAC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.092"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1015
                     /locus_tag="ACHUPM_002374"
     CDS             <1..1015
                     /locus_tag="ACHUPM_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0870126.1"
                     /translation="KISSDGCLKKEQMVVSAIASTPQLPMLGEKRLVSSVTKEDLLFV
                     RRDLLTGYQKLSNGKISSIKGRSVVTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPL
                     KKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKART
                     ITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREY
                     GRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYA
                     CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHK
                     KVG"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHUPM_002375"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHUPM_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFR0870127.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="ACHUPM_002376"
     CDS             1797..1904
                     /locus_tag="ACHUPM_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR0870128.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHUPM_002377"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHUPM_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR0870129.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHUPM_002378"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHUPM_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR0870130.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4696)
                     /gene="yehY"
                     /locus_tag="ACHUPM_002379"
                     /pseudo
     CDS             complement(3540..4696)
                     /gene="yehY"
                     /locus_tag="ACHUPM_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(4703..5620)
                     /gene="osmF"
                     /locus_tag="ACHUPM_002380"
     CDS             complement(4703..5620)
                     /gene="osmF"
                     /locus_tag="ACHUPM_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR0870131.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5872..8169)
                     /gene="bglX"
                     /locus_tag="ACHUPM_002381"
     CDS             complement(5872..8169)
                     /gene="bglX"
                     /locus_tag="ACHUPM_002381"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR0870132.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8365..10080
                     /gene="dld"
                     /locus_tag="ACHUPM_002382"
     CDS             8365..10080
                     /gene="dld"
                     /locus_tag="ACHUPM_002382"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0870133.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10119..11051)
                     /gene="pbpG"
                     /locus_tag="ACHUPM_002383"
     CDS             complement(10119..11051)
                     /gene="pbpG"
                     /locus_tag="ACHUPM_002383"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR0870134.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMLVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11225..11812)
                     /locus_tag="ACHUPM_002384"
     CDS             complement(11225..11812)
                     /locus_tag="ACHUPM_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR0870135.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11982..12560
                     /locus_tag="ACHUPM_002385"
     CDS             11982..12560
                     /locus_tag="ACHUPM_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0870136.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12690..13451)
                     /locus_tag="ACHUPM_002386"
     CDS             complement(12690..13451)
                     /locus_tag="ACHUPM_002386"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0870137.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13504..14940)
                     /gene="mdtQ"
                     /locus_tag="ACHUPM_002387"
     CDS             complement(13504..14940)
                     /gene="mdtQ"
                     /locus_tag="ACHUPM_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFR0870138.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
BASE COUNT         3707 a         4148 c         3806 g         3526 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgcagtg
     3781 gtaactgaac atgcaggaat cgctgcgccc cgctcatgcc catcgctctg gcgctctcca
     3841 gcacatcgcg cgggatctgg ttcaaaccga ctaccacgcc gcgcaccagc ggcagcaagg
     3901 catagagcac cagcgcaatc agtgcgggtg tcattccggt tcctgctatg ccgagcgtcc
     3961 ccagccacgg aaaggccgta accagcgcgg caagcggcgc aatcaacagg ccaaagagcg
     4021 ccaccgaagg cacggtctga atgacattga gcagagaaaa aattgccccc tgccgagcag
     4081 tggaaaagta gcaccagatg cccaacggca caccaatcac taacgcaggc agcaccgcac
     4141 caaacagcaa cgtcagatgt tgtgccagcg cgtcgtcaaa cacatcctga cggttggcgt
     4201 attctttcat tagtgagaga tcgttaagcg tgccggagta cagcaaccac agcggaataa
     4261 tggcaatctg catatgcaac aaccagcgcc acagcggatg cgtggagatt cggcggatgg
     4321 catcactaca ggccagcaat gccagcgccg cggccagcca gaaaccactg ccgaggctgg
     4381 tacgcgccag cgcactgcca ttttgcgcca gttgggtcgc cgcctttcct gctccccaca
     4441 ccagcaatac gaagacgaat tgcgccagaa tgagtgcaaa aatgctgcct tttttagcgg
     4501 gaataaaaca ggctgtcagc caggcgcaac caacgcccac cagcatccag atcgtttgcg
     4561 gccacagttg ccagagatga cgcccttcac ccgagactaa acgattaggc gcgtaactga
     4621 taaacggcag cgccgctgcg attgccgtca gcaacagcag cagcgccaga acaggattaa
     4681 tacgtagata agtcacggga aattacttcg tccacccttt ttgtttcagg tagtcggcag
     4741 ccactttttt ggcatccagt ccttccactg caatgctggc attcagttgc tgcaatgttt
     4801 ttgcatcgag gctggcgaag actggctgta gccactgtgc catttgcgga tactctttca
     4861 gcaccgactc acgcaccact ggtgcaggcg cgtagatagg ttgcacgcct ttcggatcgc
     4921 ttaaggtttg cagccccagc gccgcgaccg gaccgtcagt gccgtaagcc attgcggcat
     4981 taacgccaga agtttgctgg gcagcggctt taatcgttac cgccgtgtcg ccgccagcca
     5041 gtgacagtaa ctgatcctga tcgagtttaa agtcgtaggc tttttcaaac gcgggtaacg
     5101 catcggcgcg ttcgataaac tctgccgagg ctgccagttt gaaggtgccg ccctctttca
     5161 gataacgact caggtcggca agcgaagtga gtttgttttt ctctgccaca tcctgacgca
     5221 cggcgatggt ccaggtgtta ttcgctggtg cgggcgtcag ccagattaac ttgttttgct
     5281 ctgcatcgag ttttttgact ttctcgtaac cttgctgcgc gtttttccat gccgcatcgt
     5341 tttcatcttt aaagaagaaa gcgccattac cggtatattc cggatagata tccagttcac
     5401 cggaagtaat cgccccgcgc accacaggag tcgttccgag ttgcacttta ttcaccgtcg
     5461 gtacgccgtg gctttccagc acctgcaaga tgatgttgcc gagtagcgcg ccttcggtat
     5521 cgatttttga accgacttta acgggggaag ccgcctgtag cggcaggctc acggctgcca
     5581 acataaccag tgaacctgcc cagacctttg agagtggcat gatgctttcc tcattctttt
     5641 actgttgttt tcagcgaatt aagagaaaag catagttgat aatgacagag ttagcctgag
     5701 cggaaggatt cagttgcaga atcagataaa tacttaaagg gcatattttg cccggcgttt
     5761 cttgccggat gcggcgtaaa cgccttatcc ggcctacaaa ggtcgaaaat tcagcaagtt
     5821 aagatatgaa catcaggcaa agggcgcaaa cgtcgcgccc ttactaaagc attacagcaa
     5881 ctcaaactcg cctttcttaa cgcgtgcgga atcagtgccg ataaagacat tgaacttgcc
     5941 aggttcggcg tcatatttca tctgttgatt ccagaacttc agcgcctcga tatcaatcgg
     6001 gaagctgaca gtctgagttt cgcccggttt cagggtgatt ttctcaaagc ctttcagctg
     6061 cttcactgga cggctcatgg aagccgtcac atcctgcaag tacatctgta ctaccgtggc
     6121 cccttcgcgt ttaccggtgt tcgtcacctg cacgctggcg gtcactttgc cgtcacgctt
     6181 catggtcggc gcagaaagtt tcacatcaga gacggtgaaa gtggtatagc tcagaccata
     6241 gccgaacgga taaagcgccc cgttagcttc atcaaaataa cgcgaagtgt atttgttcgg
     6301 cttgtcggca ttatacggac gaccggtatt cagatggctg tagtacaccg ggatctgccc
     6361 gacagaacgc gggaaggaca ttggcagctt gccggacggg ttgtaatcgc caaacaatac
     6421 atcggcaatt gcattaccgc cttcagtccc cgcaaaccag gtttccagaa tcgcatcagc
     6481 ctgctgatct tctttcacca gcgccagcgg acgcccgttc atcagcacca gcaccagcgg
     6541 tttaccggtg gctttcagcg ccgtaatcaa gtcacgttgg ctttgcggaa tagtgatatc
     6601 ggtacggctg gaggcctcgt gcgccatccc ctgtgcttca ccgaccacag ccaccaccac
     6661 atcagattgt ttcgccgtct gcaccgcttc atcaatcatc tcttgcggcg agcgcggatc
     6721 gactttgacc gcttcttcat actgattcag gaaatcgata atgcctttgt cactggtaac
     6781 gttcgcccct ttggcataca gcactttacc gttttcaccg acggcatttt taatcccggt
     6841 cagtacggtc acggattgat cggcaacacc ggctgcggac cagctgccca tcacgtcacg
     6901 tttactgtcc gccagtggcc caaccaccgc aatggtggcc gattttttca gcggtaacgt
     6961 ttcgagacgg tttttcagca acaccaggct ttcgcgcgcc acttcacgcg cttctttacg
     7021 gtgcaggcgg ctttcggcat tggtatccac cgggtcagac tctttcggcc ccaaatggct
     7081 gtatgggtcg ttaaacaatc ccatatcata tttaacgttc agtacatggc gggcagcatc
     7141 gtccagctct tccatcgtca ctttgccgga tttgatcaac ccaggcagat acttcgagta
     7201 atactcgtcg ctcatactca tgttgattcc ggatttcagc gccacgcgca ccgcatcttc
     7261 cgggtctgcc gccgtgccat gtttaatcag ctctttgatt gcaccgtgat cggaaacggt
     7321 gatgccttta aagccccact ggtcgcgcag aacatctttc agcagccagg aatcggaggt
     7381 ggctggcgtg ccgttcagcg agttcagcgc caccatcacc gcgccgctgc ctgcgtccag
     7441 ccccgctttg tacggcggca tataatcatt aaacaggcgc tgcggactca tatcgacggt
     7501 gttgtactct ttaccgcctt ctaccgcgcc gtatgcggca aagtgtttga cgctggtcat
     7561 caccgagtag cgatctgccg ggcttttacc ctgcatcgct tccaccatgg ttttacccat
     7621 tgttgaggtg agatacgtat cttcgccaaa accttcggaa gcacgtcccc agcgcggatc
     7681 gcgcgagaca tcgaccatcg gtgcccaggt catattcagg ccatcatctg ccgcttcata
     7741 agcagagaca cgtccgaccg ttttcactgc atcgaggtta aaagacgagg ccagaccgag
     7801 gctaatcggg aacaccgtgc gctgaccgtg cagcacgtcg taagcaaaga aaagaggaat
     7861 tttcaggcgg cttaattcca tcacctgatc ctgcatggcg cggatatcct gacgggttac
     7921 ggtgttgaaa atcgccccaa cctgaccgtc tttgatcatc tcgcggatcg cctctttcgg
     7981 gttatccgga ccgacgctga ttaagcgcag ctgaccaatt ttctcatcaa ctgtcatttt
     8041 cttaagcagt tcggtgacga acgcatcccg cgcttcgggc gttaatggat ggttgccgaa
     8101 taaatcatcc gccagtgctg gctgcagggc cagactcacc gcgattccta ctgaacatag
     8161 ccatttcata tggatttttc ttcctctgtt gcccgacgag gcagcgaaaa tggtgcaaaa
     8221 accgtagttt gccataagca tgatggagag agaaaaagaa tgctcagttt attgtctgaa
     8281 ttttcaaaat attcactcgc tgaattgtca tacaaggcgc tattctagtt tgtgatattt
     8341 tttcgccacc acaaggagtg gaaaatgtct tccatgacaa caactgataa taaagccttt
     8401 ttgaatgaac ttgcccgtct ggtcggtcat tcacacctgc tcaccgatcc cgcaaaaacg
     8461 gcccgctatc gcaagggctt ccgttctggt cagggcgacg cgctggctgt cgttttccct
     8521 ggctcactac tggaattgtg gcgggtgctg aaagcctgtg tcaccgccga caaaattatt
     8581 ctgatgcagg ctgccaatac aggcctgacc gaaggatcga cgccaaacgg taacgactat
     8641 gatcgcgata tcgtgatcat cagcaccctg cgtctcgaca agctgcacgt tcttggcaag
     8701 ggcgaacagg tgctggccta tccgggcacc acgctctatt cgctggaaaa agccctcaaa
     8761 ccgctgggac gcgaaccgca ctcagtgatt ggatcatcgt gtataggcgc atcggtcatc
     8821 ggcggtattt gtaacaactc cggcggctcg ctggtgcaac gtggcccggc gtataccgaa
     8881 atgtcgttat tcgcgcgtat aaatgaagac ggcaaactga cgctggtgaa ccatctgggg
     8941 attgatctgg gcgaaacgcc ggagcagatc cttagcaagc tggatgatga tcgcatcaaa
     9001 gatgacgatg tgcgtcacga tggtcgtcac gcccacgatt atgactatgt ccaccgcgtt
     9061 cgtgatattg aagccgacac gcccgcacgt tataacgccg atcctgatcg gttatttgaa
     9121 tcttctggtt gcgccgggaa gctggcggtc tttgctgtac gtctagatac cttcgaagcg
     9181 gaaaaaaatc agcaggtgtt ttatatcggc accaaccagc cggaagtgct gaccgaaatc
     9241 cgccgtcata ttctggctaa cttcgaaaat ctgccggttg ccggggaata tatacaccgg
     9301 gatatctacg atattgcgga aaaatacggc aaagacacct tcctgatgat tgataagtta
     9361 ggcaccgaca agatgccgtt cttctttaat ctcaagggac gcaccgatgc gatgctggag
     9421 aaagtgaaat tcttccgtcc gcattttact gaccgtgcga tgcaaaaatt cggtcacctg
     9481 ttccccagcc atttaccgcc gcgcatgaaa aactggcgcg ataaatacga gcatcatctg
     9541 ctgttaaaaa tggcgggcga tggcgtgggc gaagccaaat cgtggctggt ggattatttc
     9601 aaacaggccg aaggcgattt ctttgtctgt acgccggagg aaggcagcaa agcgttttta
     9661 caccgtttcg ccgctgcggg cgcagcaatt cgttatcagg cggtgcattc cgatgaagtc
     9721 gaagacattc tggcgttgga tatcgctctg cggcgtaacg acaccgagtg gtatgagcat
     9781 ttaccgccgg agatcgacag ccagctggtg cacaagctct attacggcca ttttatgtgc
     9841 tatgtcttcc atcaggatta catagtgaaa aaaggcgtgg atgtgcatgc gttgaaagaa
     9901 cagatgctgg aactgctaca gcagcgtggc gcgcaatacc ctgccgagca taacgtcggt
     9961 catttgtata aagcaccgga gacgttgcag aagttttatc gcgagaacga tccgaccaac
    10021 agtatgaatc cggggatcgg taaaaccagt aagcggaaaa actggcagga agtggagtaa
    10081 aaattacgga tggcatagta tcgccatccg tcattcactt aatcgttctg tgccgtctgc
    10141 cccgccgccg ccatttgggc ggctttttgt tttttatagc tcaacgctgc tgctggaaca
    10201 agcatcactt taccagtttc aatccaggta cgcaggcggc tggcatcggc aaaatgggta
    10261 tatttgccaa acgcatccat cactaccagc gctaccggtt tattattgat aaccgtacgc
    10321 atcaccagac aatggcccgc cgcattggta aaaccggttt tggttaactg aatattccag
    10381 ttatcgcgat acaccagatg gttagtattg cggaacggca gcgtatacgt cgggttagag
    10441 aaggttgcca tatcttcccg ggtagtactt aactgcccga tcaacggata ttgtttgctg
    10501 gcaatgagca gtttggttaa gtcacgggca gttgaaacgt tatgcaccga caatccggta
    10561 ggttcaacaa agcgcgtgtt gttcattccg agcgatttcg ctttcgcatt cattgcctta
    10621 ataaaggctt tgtaaccacc gggataatgg tgtgcaaggc ttgccgccgc gcggttttct
    10681 gaagacatca gcgccagcaa cagcatatct ttacggctga tttcgctatt cagtcgtacg
    10741 cgcgaataga cccctttcat ctccggcgtc tggctgatat ccacttttag tttttcatcc
    10801 agcggcagtc gtgcatccag cacaaccatc gcggtcatta atttgctgat agacgcaatc
    10861 ggacgcacca gatccgggtg gttcgagtag atcactttgt tggtattcag atcaacaatc
    10921 atcgcgctac cggaggcaat ttcaggttgt gaagcggtgg tagcggctgc cgttttcgca
    10981 acggcctgcg gtgccaaagg cacagccagc atcagggcca ggctaaataa agaaactcga
    11041 aatttcggca tgatgagcat tcagatagtg gttcacgcgc acaggttgcg caccgccgga
    11101 gtaaggattt actgaggcta gcgacgccat cataacgagc aaaaagtgcg atcgtcaaag
    11161 gagaatcgtg aggaaatgct gcattgctga catttacgcc agcaatgcaa cgtcaaagaa
    11221 ctttctagaa caaacgataa ccgtagcccc atagtataac ggttagggcg agcagcactt
    11281 ccagtaccag cacgccaatc gccagcgtcg aactagagaa gctaaggcct tcctctttgt
    11341 tgatattcag gaagctcgga ataccaaggt aaagcaggta gccggtgtaa aacagcgcca
    11401 ccgtgccgac cagcgcacac aaccagacca gtggataaag cgccaccaga ccgcttaaaa
    11461 acagcggagt tgcaacgtag cccgcgaaga ccatgcagtg cgcaagtgac ggacgctgcg
    11521 gataattacg cgccatccac cagatgaccc gccccattac cgcaacccca gccagcataa
    11581 cgccataaaa caggacagcc agcgccagtc cggtaaacca ggataacttc aggatagtgc
    11641 catcgccaaa attccagcca atctgtgtag tgccaatgaa ggcgcaaatc accggaatcg
    11701 ccgccatcag caaaacgtgg tgggtgtaat ggtgagaaat cgtttcgttt tcgcgattaa
    11761 tcacctgcat ttcacgatcg ggatgggaaa acagtcccca gacatggctc ataccgcccc
    11821 cttgttgtga gttcatgaac ctgacagttc aagtataagt cagcttgtga ttattttttg
    11881 ttcgctcatg taatttgcac cgtctgatgt acgtcctttt tcagcgtgaa tgatttccag
    11941 ggtgtatgct tacaggcaac caaagggaga cagactggcc tatggatctc aatacactca
    12001 tctcacaata tggttatgcc gcgctggtga tcggtagcct ggcggaaggt gaaaccgtga
    12061 ctttgctggg aggcgttgcg gcacatcagg ggctattaaa gttcccgctg gtggtacttt
    12121 ctgtggcgct tggcggcatg attggcgacc aggtgcttta tctgtgcggg cggcggtttg
    12181 gcggcaagct gttacgccgt ttctcgaaac atcaggataa aattgagcgg gcgcagaaac
    12241 ttatccaacg ccatccgtat ctgtttgtca ttggtacgcg ctttatgtat ggctttcggg
    12301 tgattggccc gacgctgatt ggtgccagcc agctgccgcc gaaaatcttt ctgccgctga
    12361 atattctcgg cgcatttgcc tgggcgttga tttttaccac tattggttac gctggtggtc
    12421 aggtgattgc gccgtggttg cacaatctcg accagcattt gaagcactgg gtctggttga
    12481 ttctggtcgt ggttctggtg gtgggcgtgc gctggtggct gaaacggcgc gggaagaaaa
    12541 agccggatca tcaggcgtaa aaccattgcc ctgggtaagg cgttcgcgcc gcatccgaca
    12601 tccggtgcac catgcctgat gcgacgcttg tgcgtcttat catgcctacc aaaattttac
    12661 ccattgaaat aaccacgcac ctcactgcgc tattctgggt tgaactgcgg attcgccaac
    12721 ataaagccgc catccactat caacgactgc ccggtggtgt aatttgcgcc ctctgagcac
    12781 agccacgcta ccaggctggc aatctcatgc gttgcgccaa aacgccgcag aggaatcgaa
    12841 ggctccgcgt cgggcttcac gtcgctgtca tccatgccat tcattggcgt ggcgatcgcc
    12901 ccaggcgcga ctgcgttcac caaaatctta tgcctgacca gctccagcgc catcgttttg
    12961 gttaacccgc cgagcgcatg tttagcggct gtgtaagcgc tggcatccgg cagcggcgta
    13021 tgttcatgta ccgacgtaat gttgatgata cgaccgccct gcccttgttt caccatctgg
    13081 cgggccgcaa tttgtgagca taaaaatgca ccatcgacat caacggtaaa aatcttgcgc
    13141 cactcatcga aagccatatc aagaaacggc gctttggtca ttgcgcccgc attattcacc
    13201 agcacatcaa tacgccccag ccgttgaatg agtttctcca gcgcctgcgc accttctggc
    13261 agtttaccga gctccagctg cacgatctcc gcacgtacgc cgtggctaac tacctcacgc
    13321 gcggtatctt ttgccccttc ttcatctgag tgccaggtaa tgccaatatc aaacccctgc
    13381 tgcgccagta ataacgcgca ctctttgccg atccccgaat cggaggcggt aataatcgca
    13441 acctgtgcca tcaagttctc cacttaacgc tgaataaacg ttaagtatag aaggcgcata
    13501 tcatcagcgt ttgtaacccc cgcccaacgc accagtgagt tgaatagaag catccagcca
    13561 ctgcccttgc aataacaggc cattggcccg ctcacgcagc gcggggattc tggcttcgct
    13621 gacgcgggaa ccaacaatga tgcccgcgtt aaagcgcgcc tgcgcaagac ctaccacacg
    13681 caaggcatcg cgctcaattt gcgcctgatg ctggtttttc tccgcaagtg tctgaacctg
    13741 actggctgcc cgcgccacgt cattcaccgc ttcaaccacc gctttgttgt agctggcgat
    13801 agacaagttg ctttcggctt ttgcgatatt gagattggcg ttaagacgac cactatcgaa
    13861 aatgggtagc gtcaggcctg ccgtaacgcc catttgctgc gcggaatgac ggaacagatc
    13921 gcttaagtgc aacgcatcct gttgcaggaa ggccatcagg ttgatgtcag gataaaatgc
    13981 cgcttttgcc gcatcaatgg tgcttagcga tgactcaacg taccagtgcg ccgcctgcaa
    14041 atctgcccgc cgggccagta aggagtaccc cagttcatca ggaagctggc ttgccacttt
    14101 cggcaacgcg accggtttaa gcttcaatga ctttgtctgg ttatttgtaa gtgcgcttaa
    14161 ccgtgcctca ataattttca ttttccccgc gacatcgttg agctgctgcc gggttttgct
    14221 ggcattaata tcggtttcca caccttcaac tgaagaagta atcccgttct gatatagctg
    14281 gcgatcggtc gcgataatgg tgttctgctc tttttctatt tgctgcaaga ccgtgtttaa
    14341 cgccgcctgg gtttgccact cccagtacag gcgggctacg ctgccagcca gcaattggcg
    14401 ggtttgctcg cgttccgccg cccgtgcttt aaccgtaccc aggcgggcag taacctccgc
    14461 ccgattcttt ccccagatat cgagatgcca gcccgccgtt aagccaaaag taccgttggt
    14521 gtaccacggg ccggtcgtac ctgcggccgg atcgttcaga gcaaacggcc ccattaagcc
    14581 ttctgccgac attttttgcc gctccatatc cgccgaaaag tcgatctgcg gaccatcctg
    14641 agtggcaact gccttcgcct gggcttcagc tagctgaatg cgctgttcag ccacctgcat
    14701 atccggtgcg ttctgtagtg cattgttaat taatgaagtg agctgattat cgtgatactc
    14761 cagccaccat tggctgtctg gccaaccatt tttcagcgcc gtgggtaatg cggtgtcaac
    14821 ttgtgcagcg ggcgtttgct ggcttaacgc ctggcgggtt tcatgcatag gcgcacaccc
    14881 ggccagcatc agtaacagcg gaaaacaggc gatggctgga taaaaggaat cacgattcat
    14941 gggggaataa tcaggtaaga aacggtgcgc ggagattacc gtgtgttgcg atatattttt
    15001 tagtttcgcg tggcaataca tcagtagcaa taaaacgaca tacccagaaa aatatagact
    15061 aagtaaatga tatcttccga tttatcttaa tcgtttatgg ataacggcaa agggcttcgt
    15121 tttttcctat acttattcag cactcacaaa taaaggaacg ccaatgaaaa ttatactctg
    15181 ggctgta
//
LOCUS       DAZPAC010000093        15135 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZPAC010000093 DAZPAC010000000
VERSION     DAZPAC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15135
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.093"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            78..242
                     /locus_tag="ACHUPM_002388"
                     /pseudo
     CDS             78..242
                     /locus_tag="ACHUPM_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            complement(280..1341)
                     /locus_tag="ACHUPM_002389"
     CDS             complement(280..1341)
                     /locus_tag="ACHUPM_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0870139.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1554..3017
                     /gene="bepA"
                     /locus_tag="ACHUPM_002390"
     CDS             1554..3017
                     /gene="bepA"
                     /locus_tag="ACHUPM_002390"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR0870140.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            3038..3397
                     /gene="arsC"
                     /locus_tag="ACHUPM_002391"
     CDS             3038..3397
                     /gene="arsC"
                     /locus_tag="ACHUPM_002391"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR0870141.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3535..4236)
                     /locus_tag="ACHUPM_002392"
     CDS             complement(3535..4236)
                     /locus_tag="ACHUPM_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR0870142.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4331..5620)
                     /gene="uraA"
                     /locus_tag="ACHUPM_002393"
     CDS             complement(4331..5620)
                     /gene="uraA"
                     /locus_tag="ACHUPM_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR0870143.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5706..6332)
                     /gene="upp"
                     /locus_tag="ACHUPM_002394"
     CDS             complement(5706..6332)
                     /gene="upp"
                     /locus_tag="ACHUPM_002394"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR0870144.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6657..7694
                     /gene="purM"
                     /locus_tag="ACHUPM_002395"
     CDS             6657..7694
                     /gene="purM"
                     /locus_tag="ACHUPM_002395"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR0870145.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7694..8332
                     /gene="purN"
                     /locus_tag="ACHUPM_002396"
     CDS             7694..8332
                     /gene="purN"
                     /locus_tag="ACHUPM_002396"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR0870146.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8503..10569
                     /gene="ppk1"
                     /locus_tag="ACHUPM_002397"
     CDS             8503..10569
                     /gene="ppk1"
                     /locus_tag="ACHUPM_002397"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR0870147.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10574..12115
                     /gene="ppx"
                     /locus_tag="ACHUPM_002398"
     CDS             10574..12115
                     /gene="ppx"
                     /locus_tag="ACHUPM_002398"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR0870148.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12154..14397)
                     /gene="pdeF"
                     /locus_tag="ACHUPM_002399"
     CDS             complement(12154..14397)
                     /gene="pdeF"
                     /locus_tag="ACHUPM_002399"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR0870149.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14579..14731)
                     /locus_tag="ACHUPM_002400"
     CDS             complement(14579..14731)
                     /locus_tag="ACHUPM_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870150.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14749..14940
                     /locus_tag="ACHUPM_002401"
     CDS             14749..14940
                     /locus_tag="ACHUPM_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR0870151.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT         4006 a         3846 c         3722 g         3561 t
ORIGIN      
        1 gttcgccatg atgcgcgacg ggacttttta taccccgcag gggtcataac atgcttgaca
       61 acttaatagg ggcacccccc ttctggcaac tggcgcacag tagcgcagac aattttccgg
      121 cactgacggt aagccatttt attaccgcca atctgctccc ggtgatgctg ggtaatatta
      181 tcggcggtgc agtgctggtg agtatgtgtt atcgggctat ttatttacgt caggaatcct
      241 gaaaaatcag cccggcgaaa cagttcgtcg ggctgaagat tattcttgcg ctatttgccc
      301 atcgggccag gcgtggatca cggctttgat cagcgtcgcc aatggaatag cgaagaatac
      361 gccccagaat ccccacaaac caccgaagat caccaccgat aaaataatca ccagcggatg
      421 caggttaacc gcttcggaga acaacaccgg tactaacaga ttaccgtcca gcgcctgaat
      481 aatcagatac actgcgaagc agctccagaa ttccgtgcct gcaccaaact ggaatagcgc
      541 cacgccaacc accggaatgg tcaccacaaa tgcgccaatg tacggaataa gtaccgagaa
      601 gccgaccagc accgccagca gcagcgaata gttcagccca aagagcaaga accccagcca
      661 agtggcgatc cccaccacga tcatctccag cactttgccg cggatatagt tggtgatttg
      721 ttgattcatc tccttccaca cctgtcctgc cagtccacgg ttgcgcggca gcacccgacg
      781 aacggcgttc agcatctgct ctttgtcttt cagcaggaag aagaccatca atggtaccag
      841 caccagatag acggcaatgg tcagcaaacc gaccagcgag gcgagggaaa ttttcaccac
      901 cgaatcgccc atggtcagca tccgactgcg catattttcg gccattgcat caataatgcc
      961 cgcgtccatt aatgccggat agcggcgcgg caacgtggcg gcaaagtcag aaagcttatt
     1021 gagcatcccc ggcatatcgc ggatcaagta gatgccctgt tgccaggcga taggcagtac
     1081 cacgaacgcc atcagtagca atataccgac gaaaaccacc aatacaatcg acgtcgccca
     1141 gcggcgggag cagccaatag attgcaggcg cacggttggc cattccagca aataggccag
     1201 cacaatagcc accagcaacg gagcaagcag gccactaaag aaaaagataa tgccaaatcc
     1261 ggcaactaaa ataaccagca aggcaatcgc ttccgggtcg ctaaaacggc ggcgatacca
     1321 ttgcatcaac atttcgagca taaagacctt ccctgaacct caagagcggg attgcgatcc
     1381 gcaattgtat cgaaatgtca caaaaaagac ttcgcttttt atgacggatt caggaaactg
     1441 aaaagtcatt tgagtgggct aatcttcgcc gttacactca aaggcggcgc ggtgggaaca
     1501 atatttcaca gtatcggtca aatgactatc ttcagaaata caggatagag gttatgttca
     1561 ggcagttgaa aaaaaacctg gttgcaaccc tcattgctgc tatgaccatt ggtcaggtag
     1621 ccccggcgtt tgccgacagc gcagacacct tgccggatat gggaacctcc gcaggaagca
     1681 cgctttccat tggtcaggaa atgcagatgg gcgactatta tgtccgccag ctacgcggca
     1741 gcgcgccgtt aattaatgac ccgctgttaa cgcaatatat taattcgctg gggatgcgtc
     1801 tggtttcgca tgccaattcg gttaagacac cgtttcattt ttttctgatc aacaacgacg
     1861 aaattaacgc ctttgctttc tttggcggca acgtggtgct gcactctgcc ctgttccgtt
     1921 attccgataa cgaaagtcaa ctggcttcag ttatggcgca cgaaatctcc cacgtcaccc
     1981 aacgtcacct ggcgcgagcg atggaagatc agcagcgcag cgcgccgctg acctgggtcg
     2041 gcgcgttagg ttctatttta ctggcgatgg ccagtccgca ggcggggatg gcggcgctga
     2101 ccggtacact ggcgggaacg cgtcagggga tgatcagttt cacccagcaa aatgaacagg
     2161 aagcggaccg cattggtatt caggtgctgc aacgctcggg attcgatccg caggcgatgc
     2221 caaccttcct cgaaaaatta ctcgatcagg cgcgttactc ctcgcgcccg ccggaaattt
     2281 tattgactca cccgttgccg gaaagtcgtc tggcagatgc ccgcaaccgt gctaatcaga
     2341 tgcgcccgat ggtggtgcag tcgtcggaag atttctatct ggcgaaagcg cgcacactgg
     2401 ggatgtataa ttccggacgt aaccagctca ccagtgattt gctggatgaa tgggcgaaag
     2461 gaaacgttcg tcagcaacga gcggcgcaat atggtcgtgc tttacaggcg atggaagcca
     2521 ataaatacga cgaggcgcgt aaaacgctgc aaccgttact ggcggcagaa cctggcaacg
     2581 catggtatct cgatctggct accgatatcg atcttgggca aaacaaagcc aatgaggcaa
     2641 tcaatcgcct gaaaaatgcc cgtgatttgc gcaccaatcc ggtgttgcag ctcaacctgg
     2701 caaacgctta tctgcaaggc ggtcaaccac aagaagcggc caatattctg aatcgctaca
     2761 cctttaataa taaagatgac agcaacggct gggatttact ggcacaggcg gaagccgcgc
     2821 taaataaccg cgatcaggag ctggctgcgc gagcagaagg ttatgcgctc gccggacgac
     2881 tcgatcaggc catttcgctg ttgagtagcg ccagttcgca ggtgaaatta ggcagccagc
     2941 aacaagcgcg ttacgatgcg cgcatcgacc agttgcgcca gctgcaggaa cgctttaagc
     3001 cttataccaa gatgtaattc gcattaaagg aagaacaatg accaaacagg taaaaatcta
     3061 ccataaccca cgctgttcaa agagccggga aacgctgaac ctgctgaaaa aaaacggcgt
     3121 ggagccagaa gtggtactct accttgagac acccgccgat gcggcaacgc tgcgcgattt
     3181 gctgaaaatg ctggggatga atagcgcccg tgaattgatg cgccagaaag aggatcttta
     3241 taaagaactg aacctggctg acagttcgct tagcgaagag gcgctgattc aggcaatggt
     3301 ggataatccg aagctgatgg agcgcccgat tgtggtcgcg aatggcaaag cgcggattgg
     3361 tcgtccaccg gagcaggtac tggaaatcgt gggttaagat gtgccccatc gctagttgaa
     3421 gcacattgcc ggatgcgacg cttgccatgt cttatccggc ctacgaatac tatggattaa
     3481 cttcgcgtag ttcggataag gcgttcgcgc cgcatccgac aataaacacc ttatctacaa
     3541 cttcagaatt tctttcacaa acggaatggt cagcttacgt tgcgcggtaa tcgacgcacg
     3601 atccaactga tccaacgtca taaatagcgt gcgcatttct ctgtcgagcc gcttcagcaa
     3661 gaaacgcccc acatcttccg gcagttcaaa accacgcaaa cgcgcgcgta actgtagcgc
     3721 ctgcaactta tcttcatcag aaagtggctg caatttgtag atctgccccc agtcgagtcg
     3781 cgacgcgaga tccggtaatc ccagattcaa ctgccgcggt ggacgatcgc cggtgatcaa
     3841 caatcgtgtt ttgcccgatt ccagaattcg attgtagaga tcgaaaatcg ccatctccca
     3901 caactcatcg cctgcaatac actcaatgtt gtcgatacag accagcgaca aatgctccat
     3961 accgtcgagc acttccggaa caaaccaggt gcgtttatcc agcgggacat agcccaccgc
     4021 atcgccacgc tgcgacaatt ccgcgcaagc cgcgtgcagc agatggctgc gccccgcgcc
     4081 ttcgcgtgcc cagagataga tgtaaccgct atgttcctga cgcagcacgt tttgcagcgc
     4141 agccagtaaa gaggagttat cccccggcca gaaacttgca aaggtttcgt cgtcaggaag
     4201 ataaagtggc aaagagagct gtgccggtgt gttcagagat acctcaacca ggatttcaca
     4261 aaatcgcgag aagtttacca cagaatccca tgatgtttga accgggcagc aacactgccc
     4321 ggtcggtaca ttatttgtct gttatatccg cgtcttctgc gtccagcacc acttcttcag
     4381 ggcgcagcac actaatcagt ttgaagatca aacttaagcc gataccgacg atggtcgcca
     4441 gcgccatacc tttcagctcc gccgcgccaa tgtttacctt cgcgccactg acaccgatga
     4501 tcaaaatcac ggaagtcagg atcaggttct gtgctttgtt gtaatccact tttgattcga
     4561 tcaaaacacg aataccggaa gcaccgatga caccataaag cagcagcgaa acgccgccca
     4621 tcaccggcaa tgggatcatc tggatagcgg cagccagttt accgacgcag gaaagcagga
     4681 tagcgaaaat cgccgcccca ccgataaccc aggtactgta aacacgggtg atcgccatca
     4741 cgccaatgtt ttctccgtaa gtggtatttg gcgtagagcc aaagaagccg gagatcacag
     4801 tcgacaggcc gttagcaaac atcgaacggt gcagacctgg atcgcgcagc agatcttttt
     4861 tgacgatatt agccgttact accaggtgcc ctacgtgttc ggcaataacc actaacgccg
     4921 ctggcagaat agtcagaatg gcaaaccact cgaagcgcgg cgtatagagg gttggcagcg
     4981 caaaccagtg agcattaata atcggcgtgg tatcgacaat tcccattgcg aaagagagcg
     5041 cgtaccccac cagcacgcca attaaaatcg ggataattgc caggaaacca cggaacagca
     5101 cggaacctaa aaccgtgacc gccagggtgg taatagagat gatgatggtt ttggagtctg
     5161 gcgtttgccc ttcagccggg agtaaacccg ccataccggc agctacgccc gccagctcca
     5221 gaccgatgac ggcaacgatt gcgcccattg ccgcaggtgg aaacagcacg tccagccagc
     5281 cggtccccgc tttcttcacg ataaaagaaa ccaggcagaa cagcacgccg cacataataa
     5341 agccgcccag cgcgacttca taccctaacg gcaacagtaa caataccggt gaaataaagg
     5401 caaagctgga accaagataa gccggaattt tccctttaca gatgaagaga tacagcagcg
     5461 ttccaatacc gttaaataac agtacagtcg ccgggttaat atgaaataag acgggcacca
     5521 ggacggttgc accaaacatg gcgaacaaat gttgcaaact aagcgggatt gtctgtaaaa
     5581 gtggcggtct ttcactcacc ccgatagcac ggcgcgtcat agtattatcc tctgtattat
     5641 gtgttatagg cgctttactc aaaaaaaagc cgactcttaa agtcggcttt aattattttt
     5701 attctttatt tcgtaccaaa gattttgtca ccggcatcgc cgaggcccgg aataatgtat
     5761 ccgtgctcgt tcagtccctg gtcaatcgat gcggtataca gttcgacgtc cgggtgcgct
     5821 ttttccagcg cagcgatacc ttctggcgca gctaccagca ccagaacttt gatgctgctg
     5881 cagcccgctt ttttcagcag gtcgatggtc gcgataacgg aaccaccggt tgccagcatt
     5941 gggtcaacga tcagcgccat acgctcatcg atgttagaaa ccagtttctg gaagtacggt
     6001 accggctcca gcgtttcttc attacggtac ataccgacaa cgctgatgcg cgcgctcgga
     6061 acattttcca gcacaccgtc catcatcccc agaccggcac gcagaattgg cacaacggta
     6121 attttcttac ctttgatctg gtcgatttct accgggccgt tccagccttc gatagttact
     6181 ttttccgttt cgaggtcggc ggtcgcttcg taagtcagca ggctacccac ttcggaagcg
     6241 agttcgcgaa agcgcttggt gctgatatct tgctcacgca tcagtcccag cttgtgtttg
     6301 acgagtgggt gtttgacttc cacgatcttc atactctttc tcctttgagg ggcagccaca
     6361 aaaaaaatcg acggattata cctcctttct tcaaggcggc aatattcttt tcgttgactt
     6421 tagtcaaaat gataacggtt tgagataaag ttattttata ttcaaatggt tatgaaagaa
     6481 gattattcca tccgaaaact aacctttgcc ctggcacaag tcttctttcg ccgcgcgtct
     6541 ggggaaaaga cgtgcaaaaa ggttgtgcaa agcagtctcg aaaacgtttg ctttccctgt
     6601 tagaattgcg ccgaatttta tttttctacc gcaagtaacg cgtggggacc caagcagtga
     6661 ccgataaaac ctctcttagc tacaaagatg ccggtgttga tattgacgcg ggtaatgctc
     6721 tggttggaag aatcaaaggc gtagtgaaga aaacgcgtcg tccggaagtg atgggcggtc
     6781 tgggcggctt cggtgcgctg tgtgcattgc cgcaaaaata tcgtgaaccc gtgctggttt
     6841 ccggcactga cggcgtaggt accaagctgc gtctggcaat ggacttaaaa cgtcacgaca
     6901 ccattggtat tgatctggtc gccatgtgcg ttaatgacct ggtggtgcaa ggtgcagaac
     6961 cgctgttttt cctcgactat tacgcaaccg gaaaactgga tgttgatacc gcttcagcgg
     7021 tgatcagcgg tatcgcggaa ggatgtctgc aatcgggctg ttcactggtg ggtggcgaaa
     7081 cggcagaaat gccggggatg tatcacggtg aggattacga tgtcgcgggt ttctgcgttg
     7141 gcgtggtaga aaaatcagaa atcatcgacg gctctaaagt cagcgacggc gatgtgctga
     7201 ttgcactcgg ttccagcggt ccgcactcga acggctattc gctggtgcgc aaaattcttg
     7261 aagtcagcgg ttgtgatccg caaaccaccg aacttgatgg taagccatta gccgatcatc
     7321 tgctggcacc gacccgcatt tacgtgaagt cagtgctgga gttgattgaa aaggtcgatg
     7381 tgcatgccat tgcgcacctg accggcggcg gcttctggga aaacattccg cgcgtattgc
     7441 cagataatac tcaggcagtg attgatgaat cttcctggca gtggccggaa gtgttcaact
     7501 ggctgcaaac ggctggtaac gttgagcgcc atgaaatgta tcgcaccttc aactgcggcg
     7561 tcgggatgat tatcgccctg cctgctccgg aagtggacaa agccctcgcc ctgctaaatg
     7621 ccaacggtga aaacgcgtgg aaaatcggta tcatcaaagc ctctgattcc gaacaacgcg
     7681 tggttatcga ataatgaata ttgtggtgct tatttccggc aacggaagta atttacaggc
     7741 aattattgac gcctgtaaaa ccaacaaaat taaaggcacc atacgggcag ttttcagcaa
     7801 taaggccgac gcgttcggcc ttgaacgcgc ccgccaggcg ggtattgcaa cgcatacgct
     7861 catcgccagc gcgtttgaca gtcgtgaagc ctatgaccgg gagttgattc atgaaatcga
     7921 catgtacgca cccgatgtgg tcgtgctggc tggttttatg cgcattctca gcccggcgtt
     7981 tgtctcccac tatgccgggc gtttgctgaa cattcaccct tctctgctgc cgaaatatcc
     8041 cggattacac acccatcgtc aggcgctgga aaatggtgat gaagagcacg gtacatcggt
     8101 gcatttcgtc accgatgaac tggacggtgg cccggttatt ttacaggcga aagtcccggt
     8161 atttgctggt gatacggaag atgacatcac cgcccgcgtg caaacccagg aacacgctat
     8221 ttatccactg gtgattagct ggtttgccga tggtcgtctg aaaatgcacg aaaacgccgc
     8281 gtggctggat ggtcaacgtc tgccgccgca gggctacgct gccgacgagt aatgccccgt
     8341 agttaaagcg ccagctctgc cgctggcgtt tttcaattca cctgtaaatc gcaagctcca
     8401 gcagtttttt tccccctttt ctggcatagt tggacatctg ccaatattgc tcgccataat
     8461 atccaggcag tgtcccgtga ataaaacgga gtaaaagtgg taatgggtca ggaaaagcta
     8521 tacatcgaaa aagagctcag ttggttatcg ttcaatgaac gcgtgcttca ggaagcggcg
     8581 gacaaatcta acccgctgat tgaaaggatg cgtttcctgg ggatctattc caataacctt
     8641 gatgagttct ataaagtccg cttcgctgaa ctgaagcgac gcatcattat tagcgaagaa
     8701 caaggctcca actctcattc ccgccattta ctgggcaaaa ttcagtcccg ggtgctgaaa
     8761 gccgatcagg aattcgacgg cctctacaac gagctgctgc tggagatggc gcgtaaccag
     8821 atcttcctga ttaatgaacg ccagctctcc gtcaatcaac aaaactggct gcgtcattat
     8881 tttaagcagt atctgcgtca gcacattacg ccgattttaa tcaatcctga cactgactta
     8941 gtgcagttcc tgaaagatga ttacacctat ctggcggtgg aaattatccg tggcgatacc
     9001 atccgttacg cgctgctgga gatcccatca gataaagtgc cgcgctttgt gaatttaccg
     9061 ccagaagcac cgcgtcgacg caagccgatg attcttctgg ataacattct gcgttactgc
     9121 cttgatgata ttttcaaagg cttctttgat tatgacgcgc tgaatgccta ttcaatgaag
     9181 atgacccgcg atgccgaata cgatttagtg catgagatgg aagccagcct gatggagttg
     9241 atgtcttcca gtctcaagca gcgtttaact gctgagccgg tgcgttttgt ttatcagcgc
     9301 gatatgccca atgcgctggt tgaagtgtta cgcgaaaaac tgactatttc ccgctacgac
     9361 tccatcgtcc ccggcggtcg ttatcataat tttaaagact ttattaattt ccccaatgtc
     9421 ggcaaagcca atctggtgaa caaaccactg ccgcgtttac gccatatttg gtttgataaa
     9481 gcccagttcc gcaatggttt tgatgccatt cgcgaacgcg atgtgttgct ctattatcct
     9541 tatcacacct ttgagcatgt gctggaactg ctgcgtcagg cttcgttcga cccgagcgta
     9601 ctggcgatta aaattaacat ttaccgtgtg gcaaaagatt cacgtatcat cgactcgatg
     9661 atccacgctg cgcacaacgg caagaaagtg accgtggtgg ttgagttaca ggcgcgtttc
     9721 gacgaagaag ccaacattca ctgggcgaag cgcctgaccg aagcaggcgt gcacgttatc
     9781 ttctctgcgc cggggctgaa aattcacgcc aaactgttcc tgatttcacg taaagaaaac
     9841 ggtgaagtgg tccgttacgc acacatcggg accgggaact ttaacgaaaa aaccgcgcgt
     9901 ctttatactg actattcgtt gctgaccgca gatgcgcgta tcaccaacga agtacggcgg
     9961 gtatttaact ttattgaaaa cccataccgc ccggtgacat ttgattattt aatggtgtca
    10021 ccgcaaaact ctcgccgtct gttatatgaa atggtagacc gcgaaatcgc caacgcgcag
    10081 caagggctgc ccagtggtat caccctgaag ctaaataacc ttgtcgataa aggcctggtt
    10141 gatcgtctgt atgtggcctc cagctccggc gtaccggtta atctgctggt tcgcggaatg
    10201 tgttcgctga tccccaatct ggaaggcatt agcgacaaca ttcgtgccat cagtattgtt
    10261 gaccgttacc ttgaacatga ccgggtttat atttttgaaa atggcggcga taaaaaggtc
    10321 tacctttctt ccgccgactg gatgacgcgc aatattgatt atcgtattga agtggcgaca
    10381 ccgctgctcg atccgcgcct gaagcagcgg gtgctggaca tcatcgacat attgttcagc
    10441 gatacggtca aagctcgtta tatcgataaa gaactcagta atcgctacgt tccccgcggc
    10501 aatcgccgca aagtacgggc gcagttggcg atttatgact acatcaaatc actcgaacaa
    10561 cctgaataac cctatgccaa tacacgataa atcccctcgt ccgcaggagt ttgctgcggt
    10621 cgatcttggt tcaaacagtt ttcacatggt catagcccgt gtggtagatg gtgccatgca
    10681 gattattggc cgcctgaaac agcgggtgca tctggcggac ggcctggggc cagataatat
    10741 gttgagtgaa gaggcaatga cgcgcggttt gaactgtctg tcgctgtttg ccgaacggct
    10801 acaagggttt tctcctgcca gcgtctgtat agtcggcacc catacgctgc gtcaggcgct
    10861 gaacgccact gactttctga aacgcgcgga aaaggtcatt ccctacccga ttgaaattat
    10921 ttccggtaat gaagaagccc gtctgatttt tatgggcgtg gaacataccc aaccggagaa
    10981 aggtcgcaaa ctggttattg atattggcgg cggatctacg gaactggtga ttggtgaaaa
    11041 tttcgaaccg attctcgttg aaagccgccg gatgggttgt gtcagctttg cccagcttta
    11101 cttccctggc ggggtcatca ataaagagaa ttttcagcgc gctcgtatgg cggcagcaca
    11161 aaaactggaa actttaacct ggcaattccg tattcagggc tggaacgtgg caatgggcgc
    11221 ttccggtacc ataaaagccg cccatgaagt gttaatggaa atgggcgaga aagacgggat
    11281 aattaccccg gaacgtctgg aaaaactggt aaaagaagtt ttacgtcacc gtaatttcgc
    11341 atcgctgagt ttaccgggtc tttccgaaga gcggaaaaca gtcttcgttc ctggactggc
    11401 gattttatgc ggtgtgtttg atgctttagc catccgtgaa ctgcgccttt ctgacggggc
    11461 gttacgcgaa ggcgtactgt acgaaatgga aggacgtttc cgtcatcagg atgtgcgtag
    11521 tcgcaccgcc agcagcctcg ccaaccagta tcacatcgac agcgaacagg cccgacgggt
    11581 gctggatacc actatgcaaa tgtacgaaca gtggcgggaa cagcaaccga agctggcgca
    11641 tccgcaactg gaggcgctac tgcgatgggc cgccatgctg catgaggtcg ggttgaatat
    11701 caaccacagc ggtttgcatc gccactccgc ttatattctg caaaacagtg acttgccggg
    11761 ttttaatcag gaacagcagc tgatgatggc gacactggtg cgctatcacc gtaaagcgat
    11821 taaactcgac gatctgccgc gctttacctt gtttaagaag aaacagttcc tgccactgat
    11881 tcagctattg cgccttggcg tattactcaa caatcaacgt caggcaacca ccacaccgcc
    11941 aacattgaca ctgattactg atgacagtca ctggacactg cgtttcccgc atgactggtt
    12001 tagtcagaat gcgttggtac tgcttgatct ggaaaaggag caagaatact gggaaggcgt
    12061 ggctggctgg cggttgaaaa ttgaagaaga aagtacacct gaaatcgccg cttaatgctt
    12121 tgcgggccga cgagaaatgt cggcccgcat tattcaggca ctttcgcgaa tgggttcgat
    12181 ttcattcaac gtatcaatta acggctgcgg cttaccaata agataaccct gcatataatc
    12241 gatccccaaa gagagcaccg cctcgcggat ctcttcgttt tcaacgtact ctgccactac
    12301 ccgcattttc ttcattcgcg ccagatggca aatcgacgcc acaatctgat aatccagact
    12361 atttgacaca atattgcgga taaagctgcc gtcaatttta agcagatcgg cattcacatt
    12421 tttaagccgt gcatagctgg cgtagccggt gccgaaatca tcaatcgcaa tctggcagcc
    12481 taattcctga agatgctgca aggtgatttg cgcctgctta acattggtca gagcattact
    12541 ttcggtgact tcaaaaataa gttgccacgc ttcaatctga tatttagcca gcaactgact
    12601 gacttcaaca ggaaaacgag cctgacatac cgaggttgga gacagattaa tagcaaaacg
    12661 gtgagcgggc atcttcgctc tgttttcagc cataaattgc agcgtatgct caatgaccca
    12721 catgtcgata ctcgacgata aaccaaattc gtgcgcgacc ggcaagaagc tatcggggac
    12781 gatcagttca tcattctcac ctttcatgcg cagaagaatt tcatggtaaa catcaccacg
    12841 cataccggta atcggctggg ccatcaggaa aaaatggttg tgttccagcg cctgctgtag
    12901 tcgattcatc atcgcgactt tatctttcaa ttcgcgttgc aaatacattg ccccgcgacg
    12961 ctgcatattt tccggggcgt tggtcacgat ggaaagttcg gcgaccgtat ttagctctcc
    13021 cagcagcagg tagatatgat tcactggcga gcgcacatag cagtaactga cgccaatctg
    13081 cggttgcatc ggcatcccat cccaaaagaa acgaaattgc ttgagatgac tatccagtgc
    13141 ggtaatgcgc tcctggtgcg attctgtatt cagtcgcagc gcgagatcgt tacccgaaag
    13201 ctgataaaca tcttcacccg gttccagcaa gggtgacagc cagtgagaaa gtttttgctt
    13261 gtattgaatc cgcagcatga tgccatagtt cttaaccagc atttccatgc cagggatgcg
    13321 taaataacaa agcgcagacc agggagcatc acgcaacgcg cgattcaggg cgcgaacatt
    13381 tggcagatga accaccgggt ccatatacgc aagccgctga atgcgtctga ctaccgctct
    13441 ctgacgggtt gccagtacag ccatataatt gacaataaaa gagaatacca gataactgga
    13501 ggaggttatg gtcagctgcg tggtatagcc aggataaatg ggaatgtaat tttgatagct
    13561 gtggatgctg atcatcaaca cgaccgccca gagcagcgaa atcagcttat aaccatagcg
    13621 catcgctccc cacatcatca ggggcagcaa taatgacaag gtataattgg tgctaaaaat
    13681 tgtgcttttt tcatttaacg gcatgcataa cagcaacaat aaagcaccta atgccagtag
    13741 ccagagcgcg aactcttttt tggtgacttt ggcatcaacc tgctgtttta attgcgaata
    13801 atagctacgt aaataaaatg gatttcgcac tacccgaatg atgaagtagc acagcgggac
    13861 accgatcaga ttacccacca gtaaggcctg ataattgatt aaagtcccga ggttaaaagg
    13921 catgacaccg accagatttt ctctgctcgc cagtaatcct acaaatgcag caaactggaa
    13981 aagtatcaga aacagcgttg caggaaacac aatctgccag aatatacgct gggaaattaa
    14041 acgggtatca ccatgtgaaa cgttgttacg ccggggagta aagaccctgt aaccgcccca
    14101 acagagaatg ataatgataa taaaattagc cgttaatgat agcgtttcgt aaaaacccag
    14161 ctctgcatat ttgcggacaa aaatccctaa ggcgatccct ggcaatgccg cccagctgaa
    14221 aaacatcatc atgctgatca tcagtgccag aggcagataa aagagaaaaa cctcaccgga
    14281 agaaatatgc gcgaaagtat taatgtgggc gaaaatgggc aagattaaag aaggcaggaa
    14341 cagcggaagc ccccaccatt tatcacgcat ttttatataa gttgcattca gtttcataga
    14401 tgctcagcag aatcccccac atcctgaagg aggtgtattc agacaggcat cccacctgac
    14461 ttcgaatgat gattattcat cactatagag agcattgatt ctaagtgtca tatgaaagtt
    14521 ccaattgata tatatcaaac aaaataaccc tgattaatga attattacgt ttatcatgtt
    14581 aattcatcat tattacatca tcattgtaaa taattaaatt aacttccata acattaaaat
    14641 atgtatccac tgacgttttt ttacataacg aagaattgac cattttgtcc tgttgtgcct
    14701 taatgtaagt accgtccaca gcgtgggaca tacttcaagg aaccttttgt gagtcaggca
    14761 accagtatgc gaaaacgaca ccgatttaac agtcgcatga cccgtatcgt actgctcatc
    14821 agctttatct tcttctttgg ccgttttatc tactcgtccg tcggtgcctg gcagcaccat
    14881 cagagcaaaa aagaagctca gcaatccaca ctctccgtcg aatcaccggt acaacgttag
    14941 cggttacctt cttcactttc acagaacata acggcacttc gctgtcggat gcttttgctc
    15001 tttgggatta tcaaagcggc agatattctt tcatcttaaa ttttacgtct ttgtcctgac
    15061 tgatgtttat cctgtttggc tgcggaataa atataaaatt aatatatatg ttgtaatgat
    15121 atatttttat aaatt
//
LOCUS       DAZPAC010000094        15084 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZPAC010000094 DAZPAC010000000
VERSION     DAZPAC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.094"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="yjeJ"
                     /locus_tag="ACHUPM_002402"
     CDS             <1..781
                     /gene="yjeJ"
                     /locus_tag="ACHUPM_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR0870152.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHN
                     LFDHYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVP
                     CQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(984..1337)
                     /locus_tag="ACHUPM_002403"
     CDS             complement(984..1337)
                     /locus_tag="ACHUPM_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR0870153.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1475..3121)
                     /gene="groL"
                     /locus_tag="ACHUPM_002404"
     CDS             complement(1475..3121)
                     /gene="groL"
                     /locus_tag="ACHUPM_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR0870154.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3165..3458)
                     /locus_tag="ACHUPM_002405"
     CDS             complement(3165..3458)
                     /locus_tag="ACHUPM_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR0870155.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3734..4990
                     /gene="yjeH"
                     /locus_tag="ACHUPM_002406"
     CDS             3734..4990
                     /gene="yjeH"
                     /locus_tag="ACHUPM_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR0870156.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5012..5482)
                     /locus_tag="ACHUPM_002407"
     CDS             complement(5012..5482)
                     /locus_tag="ACHUPM_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR0870157.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5819..7255
                     /gene="aspA"
                     /locus_tag="ACHUPM_002408"
     CDS             5819..7255
                     /gene="aspA"
                     /locus_tag="ACHUPM_002408"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR0870158.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7373..8674
                     /gene="dcuA"
                     /locus_tag="ACHUPM_002409"
     CDS             7373..8674
                     /gene="dcuA"
                     /locus_tag="ACHUPM_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR0870159.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8790..9128
                     /gene="cutA"
                     /locus_tag="ACHUPM_002410"
     CDS             8790..9128
                     /gene="cutA"
                     /locus_tag="ACHUPM_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR0870160.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9104..10801
                     /gene="dsbD"
                     /locus_tag="ACHUPM_002411"
     CDS             9104..10801
                     /gene="dsbD"
                     /locus_tag="ACHUPM_002411"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR0870161.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10838..11413
                     /locus_tag="ACHUPM_002412"
     CDS             10838..11413
                     /locus_tag="ACHUPM_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0870162.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11520..11595
                     /locus_tag="ACHUPM_002413"
     tRNA            11520..11595
                     /locus_tag="ACHUPM_002413"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11553..11555,aa:Phe,seq:gaa)
     gene            11838..11952
                     /locus_tag="ACHUPM_002414"
                     /pseudo
     CDS             11838..11952
                     /locus_tag="ACHUPM_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            12212..12511
                     /locus_tag="ACHUPM_002415"
     CDS             12212..12511
                     /locus_tag="ACHUPM_002415"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR0870163.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYK"
     gene            complement(12502..12627)
                     /gene="laoB"
                     /locus_tag="ACHUPM_002416"
     CDS             complement(12502..12627)
                     /gene="laoB"
                     /locus_tag="ACHUPM_002416"
                     /inference="COORDINATES: protein motif:HMM:NF038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arginine responsive protein LaoB"
                     /protein_id="HFR0870164.1"
                     /translation="MVRLWEGEFVSGTASGIGGGEDSIISSPSSSLYQITGTIHL"
     gene            12694..13749
                     /gene="cadC"
                     /locus_tag="ACHUPM_002417"
     CDS             12694..13749
                     /gene="cadC"
                     /locus_tag="ACHUPM_002417"
                     /inference="COORDINATES: protein motif:HMM:NF007540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFR0870165.1"
                     /translation="MVFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVN
                     KSCNSWSSPYQLSYAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVN
                     QRQYRAQQCFMSVKLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQR
                     MQETLQQILPHRGALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEK
                     ALVDIVRHSQHPLDEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKIDESY
                     QAINTGIDLEMSWLNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQ
                     TSVPYVVPYLDKFLASE"
     gene            14114..>15084
                     /gene="cadB"
                     /locus_tag="ACHUPM_002418"
     CDS             14114..>15084
                     /gene="cadB"
                     /locus_tag="ACHUPM_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR0870166.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3708 a         3764 c         3616 g         3996 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
     1021 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
     1081 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
     1141 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
     1201 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
     1261 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
     1321 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
     1381 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
     1441 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
     1501 tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa
     1561 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
     1621 gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
     1681 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
     1741 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
     1801 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
     1861 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
     1921 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
     1981 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt
     2041 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
     2101 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
     2161 tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga
     2221 tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
     2281 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
     2341 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
     2401 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
     2461 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
     2521 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
     2581 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
     2641 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
     2701 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt
     2761 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag
     2821 tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg
     2881 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
     2941 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
     3001 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
     3061 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
     3121 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
     3181 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
     3241 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
     3301 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
     3361 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
     3421 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
     3481 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
     3541 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
     3601 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
     3661 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
     3721 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
     3781 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
     3841 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
     3901 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt
     3961 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
     4021 tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
     4081 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
     4141 cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
     4201 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
     4261 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
     4321 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
     4381 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
     4441 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
     4501 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
     4561 tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
     4621 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
     4681 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt
     4741 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
     4801 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
     4861 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
     4921 gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
     4981 aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt
     5041 cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
     5101 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
     5161 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
     5221 gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag
     5281 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
     5341 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc agcaataccc
     5401 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
     5461 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
     5521 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
     5581 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
     5641 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
     5701 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
     5761 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
     5821 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
     5881 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
     5941 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
     6001 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
     6061 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
     6121 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
     6181 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
     6241 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
     6301 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
     6361 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
     6421 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
     6481 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
     6541 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
     6601 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
     6661 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
     6721 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
     6781 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
     6841 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
     6901 gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa
     6961 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
     7021 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
     7081 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
     7141 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
     7201 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
     7261 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     7321 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
     7381 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
     7441 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
     7501 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
     7561 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
     7621 cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg
     7681 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
     7741 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
     7801 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
     7861 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
     7921 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
     7981 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
     8041 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
     8101 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
     8161 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
     8221 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
     8281 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
     8341 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
     8401 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
     8461 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
     8521 ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa
     8581 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
     8641 cttcggcttc gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc
     8701 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
     8761 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
     8821 ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag
     8881 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
     8941 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
     9001 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
     9061 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
     9121 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
     9181 acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
     9241 tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
     9301 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
     9361 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
     9421 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
     9481 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
     9541 agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc
     9601 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
     9661 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
     9721 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
     9781 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
     9841 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
     9901 actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc
     9961 acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac
    10021 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    10081 cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    10141 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    10201 ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg
    10261 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    10321 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    10381 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    10441 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    10501 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt
    10561 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    10621 tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    10681 ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    10741 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    10801 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    10861 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    10921 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    10981 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    11041 gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    11101 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    11161 cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    11221 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    11281 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    11341 cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    11401 tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    11461 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    11521 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    11581 cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag
    11641 gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc
    11701 cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat
    11761 agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt
    11821 catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat
    11881 tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat
    11941 ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct
    12001 ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc
    12061 tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac
    12121 caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt
    12181 gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga
    12241 atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga
    12301 gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag
    12361 ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca
    12421 gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat
    12481 cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga
    12541 agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac
    12601 aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc
    12661 agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt
    12721 atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg
    12781 cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc
    12841 tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca
    12901 cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg
    12961 agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa
    13021 caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa
    13081 cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta
    13141 tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg
    13201 cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc
    13261 gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca
    13321 gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac
    13381 cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg
    13441 ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa
    13501 ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg
    13561 ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct
    13621 gatgcatatc tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa
    13681 aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct
    13741 tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta
    13801 tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt tattacataa
    13861 atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc ttttcatgat
    13921 atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta cagaaacttt
    13981 tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc ttatcgccag
    14041 tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc aaaaatgaaa
    14101 ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta ccggtgttgt
    14161 tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag caagtatcgg
    14221 tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc tggcgtatgt
    14281 atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt atgccggaga
    14341 aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta actggattgg
    14401 taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc cagtattaaa
    14461 tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta cctttgtaaa
    14521 tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc tggttcttat
    14581 tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa cttatgcagc
    14641 taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta ttctgctcta
    14701 cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg ttaaaaaccc
    14761 gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta ttgtttacat
    14821 cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg cttccggtgc
    14881 tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc tggtttctgc
    14941 attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg taggccaggc
    15001 aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag tcgacagcaa
    15061 cggtattccg aaaaaaggtc tgct
//
LOCUS       DAZPAC010000095        15035 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZPAC010000095 DAZPAC010000000
VERSION     DAZPAC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15035
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.095"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            86..829
                     /locus_tag="ACHUPM_002419"
     CDS             86..829
                     /locus_tag="ACHUPM_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0870167.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(886..2319)
                     /gene="bglA"
                     /locus_tag="ACHUPM_002420"
     CDS             complement(886..2319)
                     /gene="bglA"
                     /locus_tag="ACHUPM_002420"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR0870168.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            2364..2675
                     /gene="yqfB"
                     /locus_tag="ACHUPM_002421"
     CDS             2364..2675
                     /gene="yqfB"
                     /locus_tag="ACHUPM_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFR0870169.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            2839..3498
                     /locus_tag="ACHUPM_002422"
     CDS             2839..3498
                     /locus_tag="ACHUPM_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR0870170.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(3694..4674)
                     /gene="ygfZ"
                     /locus_tag="ACHUPM_002423"
     CDS             complement(3694..4674)
                     /gene="ygfZ"
                     /locus_tag="ACHUPM_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR0870171.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            4917..5183
                     /gene="sdhE"
                     /locus_tag="ACHUPM_002424"
     CDS             4917..5183
                     /gene="sdhE"
                     /locus_tag="ACHUPM_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR0870172.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            5164..5571
                     /locus_tag="ACHUPM_002425"
     CDS             5164..5571
                     /locus_tag="ACHUPM_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR0870173.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(5611..6132)
                     /gene="fldB"
                     /locus_tag="ACHUPM_002426"
     CDS             complement(5611..6132)
                     /gene="fldB"
                     /locus_tag="ACHUPM_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR0870174.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            6244..7140
                     /gene="xerD"
                     /locus_tag="ACHUPM_002427"
     CDS             6244..7140
                     /gene="xerD"
                     /locus_tag="ACHUPM_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR0870175.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            7165..7875
                     /gene="dsbC"
                     /locus_tag="ACHUPM_002428"
     CDS             7165..7875
                     /gene="dsbC"
                     /locus_tag="ACHUPM_002428"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR0870176.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            7881..9614
                     /gene="recJ"
                     /locus_tag="ACHUPM_002429"
     CDS             7881..9614
                     /gene="recJ"
                     /locus_tag="ACHUPM_002429"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR0870177.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            9705..10803
                     /gene="prfB"
                     /locus_tag="ACHUPM_002430"
     CDS             join(9705..9779,9781..10803)
                     /gene="prfB"
                     /locus_tag="ACHUPM_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR0870178.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            10813..12330
                     /gene="lysS"
                     /locus_tag="ACHUPM_002431"
     CDS             10813..12330
                     /gene="lysS"
                     /locus_tag="ACHUPM_002431"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR0870179.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(12373..12921)
                     /gene="idi"
                     /locus_tag="ACHUPM_002432"
     CDS             complement(12373..12921)
                     /gene="idi"
                     /locus_tag="ACHUPM_002432"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR0870180.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            12976..13047
                     /locus_tag="ACHUPM_002433"
     CDS             12976..13047
                     /locus_tag="ACHUPM_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870181.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(13044..13169)
                     /gene="yqfG"
                     /locus_tag="ACHUPM_002434"
     CDS             complement(13044..13169)
                     /gene="yqfG"
                     /locus_tag="ACHUPM_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR0870182.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(13171..14619)
                     /gene="uacT"
                     /locus_tag="ACHUPM_002435"
     CDS             complement(13171..14619)
                     /gene="uacT"
                     /locus_tag="ACHUPM_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR0870183.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         3757 a         3733 c         3951 g         3594 t
ORIGIN      
        1 aaaagcgtgc caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac
       61 gaattaattt ttctcggaaa aacaaatggc tatagcactt gtgaccgatg gcagtcgcgg
      121 catcgggcgg gcaactgcat tactgttggc gaaagaagag tatacggtgg cggttaatta
      181 tcaacaaaac ctccatgcgg cgcaggaagt ggtgaactta atcacgcaag ccggtggcaa
      241 ggcattcgtg ctccaggcgg atatcagcga cgaaaatcag gtcatcgcaa tgtttacagc
      301 aatcgatcag cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac
      361 ccagtgcacc gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacat
      421 gacgggatat tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg
      481 cagtggcggc gctatcgtca atgtcttttc ggtggcctca cggttgggtt cgccagggga
      541 atatgttgat tacgcggcat cgaaaggggc gattgatacg ttaaccaccg gactatcgct
      601 ggaagtcgcc gcgcaggggg tccgcgttaa ctgcgtgcgg ccagggttta tttttaccga
      661 aatgcacgcc agcggcggcg agcctggacg cgtcgatcgc gttaagtcga acatccccat
      721 gcagcgtggt ggacaggcag aagaggtcgc gcaggccatt gtctggctac taagtgataa
      781 agcctcttac gtcacgggaa gttttatcga tttggcgggc gggaaataaa acagggaagt
      841 tgtctgaccg gatgcaacaa gtattgcatc cggtacttca tcgacttaaa gcttctcgcc
      901 gttgctggta atcacctctt tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc
      961 gccagtaccg tcgtcatgtt tattcacata gataaagccg tagcgtttgc tgtactgccc
     1021 ggtggtgaac gatacgcagt cgatgcagcc ccacggtgtg tagcccatca gatccacgcc
     1081 atcgtaagtc accgcttttt tcatctcttc gatatgggcg cgcaggtagt caatgcggta
     1141 gtcgtcgttg atgctgccat cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac
     1201 aataaacagc ggcctctgat aacgctcata cagttcgcaa agggcatagc gcagacctac
     1261 tggatcaatc tgccagcccc agtcagacgc tttaacatac gggttcggta cgctgccttc
     1321 gaaaccagag atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg tcatgtaata
     1381 gctgaaacca agataatcgc aggtgccttc gcgcagtaca tcgagatcgc cgtcttccat
     1441 tttgatgtta aatccgcgac gctcccactc gttcaacaca taggacgggt aatagccgcg
     1501 tagctgcaca tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga acatcacatc
     1561 gtccgggtta cagcagtagg gatagagcgg caccatcgcc agcatacagc cgactttcat
     1621 ctccgggtta atgcgacgcg cagctttcac cgccagggcg ctggcgacaa actggtgatg
     1681 cagcacctga tacatcgtct cttccgggtt ctcatgctcg gtataaacca cgccggagca
     1741 gcagtaaccg aacagcggtg cacgccagtt acgctggttg ttaatttcgt tgaaggtcat
     1801 ccagtatttg actttatgct tatagcgttc aaacaccact tccgcgaaac ggacaaagaa
     1861 atcaaccact ttacggttgg tccagctacc gtattgctgc accagatgca gcggcatttc
     1921 aaagtgggag agggtgatca ccggttcgat gttgtatttc agcagttcat cgaacatatc
     1981 atcgtagaac ttcagccctt cttcgtttgg ctgagcttca tcgccttttg gaaaaatgcg
     2041 cgtccaggct atggaagtcc gaaaacattt gaagcccatt tcggcaaata gcttgatgtc
     2101 ttccttgtag tgaccataaa aatcaacggc ttcatggttt ggatagtatt tgcctggcaa
     2161 gacttctttg gtgatttcgc gcggcacgcc gtgtgcgcca ccggtcagaa cgtcacaaat
     2221 gctcggccct ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg caactgcgcc
     2281 gccccataag aaatctttct gtaaggtgag ttttttcact atcataggct ccagatgagt
     2341 caattcttac aggaaatagt accatgcagc caaacgacat cacttttttt caacgtttcc
     2401 aggatgacat tctggctggg cgtaaaacca tcaccatccg cgacgagtct gaatcgcact
     2461 tcaaaacggg tgatgtgctt cgtgtcgggc gttttgaaga tgacggttat ttttgcacga
     2521 ttgaagtcac cgcaacctca accgtaacgc tggatacgct gacggaaaaa catgcagagc
     2581 aggaaaatat gaccctgact gaactgataa aggtcattgc cgacatctat cccggtcaga
     2641 cacagtttta tgtgattgaa tttaaatgtc tttaaattga actcacgcct gttagttgaa
     2701 atttcgcatt taatttcatg atttttagga gattagtctc aaatcagaat ttattttagc
     2761 taacaggtgt tcactggaac tattctcagt tacgctggag aggtatacat gtgcgtgtac
     2821 gtttctccgg agtaagttat ggttcagaag cccctcatta agcagggata ttcgctggca
     2881 gaggaaattg ccaacagcgt cagtcacggc attgggttgg tgtttggtat cgttgggctg
     2941 gtgttgctac tggttcaggc ggtggatctc aatgccagcg ccacagcgat aaccagttac
     3001 agcctctatg gcggcagtat gatcctgctg ttcctcgctt cgacgctcta tcacgccatt
     3061 cctcatcaac gggcaaaaat gtggctgaag aaatttgacc attgcgctat ttacctgttg
     3121 attgccggaa cctacacgcc gtttttgctg gtggggctgg attctccgtt agcgcgcggg
     3181 ttgatgattg ttatctggag cctggcattg ctgggtattc tgtttaaact gaccatcgcg
     3241 caccgattca aaattttatc tctggtgacc tatctggcga tgggctggct gtcgctggtg
     3301 gtaatttatg aaatggcagt taagctcgcg gcgggcagcg ttaccttatt ggcggtaggc
     3361 ggcgtggttt attcgctcgg agtgattttc tatgtctgca aacgcattcc ctacaatcat
     3421 gccatctggc acggcttcgt gctcggcggc agtgtgtgcc actttctggc gatctatttg
     3481 tatattgggc aggcgtaatg ggtatgcctt tgtaggcctg aaaagacgcg tcagcgtcgc
     3541 atcaggcatc ggagcacaac tgccggattc ggcgtgaacg ccttatccga tctacgggcc
     3601 tcgtccctgt tgtgataaag gcatcaaatc ccagacatga taagacgcgt cagcgtcgca
     3661 tcaggcgata aatgcctgat gcgataaaga gatttactct tcgagcgaat acggcagcgg
     3721 ctcaatatgc aatgtattcg catcgtcgcg tacgcggaag atgctatccg gttccatatc
     3781 gttattcatg acgacctgta ccacgacctg accatcttcc agttttaccg cagccagcac
     3841 cgtaccggta cgacgccagt tctcgcccat tttcagctct aagtcttcac cagcttccgg
     3901 cagtcggctg gcgctacctg ccagcaacca gagcgcacgt ttattggcac cacggaattt
     3961 tgctcgcgcc accatctctt gtccggtata acagcctttc ttaaagctga taccgcccag
     4021 cgcctggaga ttggtcgcct gtgggataaa ctgcccgctg ttggcggcat caatcaccgg
     4081 gaaacccgct tcaatgttta atgccagcca ctgttggcta ttgttcagtt ccgcttcacc
     4141 gcgcagttta tcggtcagca tattagcagt agcttcatcg gttacgatca ggaaacgttc
     4201 tgccgggtat tcaaaccata gcaaagtggt cgcgccttct ttaactacct gtttttcttt
     4261 cgaaggcagt tcactaaaga tatttgccag cgcggcgcgc gcctgaaaac cggcaacacc
     4321 aagcagcaca tgctcgtcgt ctggcgcgat ggtcacttta gagaacaccg catatttttt
     4381 cagttcagtc agctgcggtt cacgcacgct gcgccgttca atccacgcaa agccatcgcc
     4441 gtcgcggaac agacgtaaat tgctccacat tttgcctttg gcgtcgcaat gggcggcgag
     4501 caggtgctga tcttctgcca tctggctgac atctgctgtc acctgaccct gcatatattt
     4561 ttcgctgtcc gcgccagtaa tggtggcaag cgcccagtca tcaagcgtca tcagcgtcag
     4621 cggtaaacgg gcagaagccg taggctgacg gggaggaaaa ggtgtaaaag ccataataga
     4681 gtcctgagtt gcttaacggc gtattaatga ctaatggtaa aagagctatg aggcaatgca
     4741 agtgctttca ctacgcgatc tgtgccttct gcgaggcgac ttccaaaaaa atgaagccat
     4801 tgaattgttg tgaattaatt tcgtgatcgc gatggtgttt actgatattc ccgtaaaaga
     4861 aggcgaaaaa cacgttacaa tagcggggta ctggattttc gtgagacagg aagaagatgg
     4921 acattaacaa caaagcccgc attcattggg catgccgccg tggtatgcgc gaactcgata
     4981 tttcaatcat gccgtttttc gaacatgagt acgacagctt aagcgatgac gaaaaacgca
     5041 tctttattcg tctgctggaa tgtgacgatc cggacctgtt taactggctg atgaatcacg
     5101 gtaaaccggc cgatgcagaa ctggaaatga tggtccgact catccagaca cggaaccggg
     5161 aacgtggtcc tgtggcaatc tgatttgcgc gtctcctggc gcgcacagtg gctttccttg
     5221 ctgattcatg ggctggttgc cgctgttatt ttactcatgc cctggccact cagttacacc
     5281 ccgttatgga tggtgttact ttcgctggtg gtgtttgatt gcgttcgcag ccagcggcgt
     5341 attaatgctc gccaggggga aattcgcttg ttgatggacg ggcgtttgcg ttggcaaggg
     5401 caggagtgga gcatcgtcaa agcaccgtgg atgattaaga gcggcatgat gctgcgttta
     5461 cgttctgatg gcggtaaacg gcaacattta tggctggcag ccgacagcat ggacgaagcc
     5521 gaatggcggg atttacggcg gattttgttg caacaagaga cgcaaagata aagatgacgg
     5581 caaagcatat gcgctaagcc gtcagtgaga tcaggcgtaa tgctctgcca tttcgttgag
     5641 gatttgctcg caccagctct gaatacgctc gtcgctaagg tcatactggt tagtttcatc
     5701 cagcgccaga cccacgaaca gttgcccgtc agcaatcacc ggtttcgggc tggtaaattc
     5761 atatccttcc gttggccagt agccgacgaa cttcacgcct ttggtcgaga gtttgtcatg
     5821 cagcataccg agcgcatcga ggaaccactc gccgtatccc agttgatcgc caagcccata
     5881 cagcgcaaca attttacctt caaggttcag gtcgtcgagc tgatcccaga cggcttccca
     5941 gtcttcctgg atttcaccaa aatcccaggt cgggataccc agaatgagca catcgtactg
     6001 ctccattaat ttcggggagt cgtccttgag gttatgtaag gtcaccagtt ctgggccgat
     6061 aatatcgcgg attttttctg ccgccatttc ggtgtaacag gtgctggaac cgtaaaaaag
     6121 acccatattc ataacgtaag cttctcgatt ctgctgtggc ttttgcagct agtgtatcag
     6181 aatcgcttta gtcacggcac aataaggcat aatggatcaa atgagtgacc ataaggggcg
     6241 caagtgaaac aggatctggc acgcatcgag cagtttcttg atgctctgtg gctggaaaaa
     6301 aatctggctg aaaatacgtt gaacgcttac cgtcgcgatc tgtcaatgat ggtggagtgg
     6361 ttgcatcacc gcgggttgac gctggcgacg gcgcaaagtg acgatttgca ggcattactg
     6421 gcagaacggc tggagggcgg gtataaagcc accagctcag cgcgtttgct gagtgcagtg
     6481 cgccgattgt tccagtatct ttatcgcgaa aagtttcgtg aagacgatcc cagtgcgcat
     6541 ctcgcttcac cgaaattgcc ccagcgtttg ccaaaagatt taagcgaagc gcaggtcgaa
     6601 cgtttattac aggcgccatt aattgatcag ccactggagc tacgcgataa agccatgctt
     6661 gaagtgttgt atgctaccgg gctgcgtgtc tctgaactgg tcgggctgac aatgagtgat
     6721 atcagcctgc gtcagggcgt agtgcgggtc attggtaaag gcaacaaaga gcgcctggta
     6781 ccgttaggtg aagaggcggt ttactggctg gaaacctatc tggaacatgg gcgtccgtgg
     6841 ctgctgaatg gtgtgtcaat tgacgtgttg tttcccagcc agcgtgcgca gcagatgacg
     6901 cgacagacct tctggcaccg cattaaacat tatgccgtgc tggcgggtat cgacagcgaa
     6961 aagctgtcac cgcatgtgtt gcgtcacgct tttgccactc atttattaaa tcatggtgcg
     7021 gatttacgcg tggtgcagat gctactgggc cacagcgatc tttccaccac gcaaatttat
     7081 acgcatgtcg ctaccgagcg tctgcggcaa cttcatcaac agcatcaccc gcgggcgtga
     7141 tgtctgaaaa gaacgggaag atttatgaag aaaggtttta tgttgtttac tttgttagcg
     7201 gcgttttcag gttttgctca ggctgatgac gcggcaattc aacaaacgtt agccaaaatg
     7261 ggcatcaaaa gcagcgatat tcagcccgcg cccgtagctg gcatgaagac agttctgact
     7321 aacagcggcg tgttgtacat caccgatgat ggtaaacata tcattcaggg gccaatgtat
     7381 gacgttagcg gcacggctcc ggtcaatgtc accaataaga tgctgttaaa gcagttgaat
     7441 gcgcttgaaa aagagatgat cgtttataaa gcgctgcagg aaaaacacgt catcaccgtg
     7501 tttactgata ttacctgtgg ttactgccac aaactgcatg agcaaatggc agactataac
     7561 gcgctgggga tcaccgtgcg ttatcttgct ttcccgcgcc aggggctgga cagcgatgca
     7621 gagaaagaaa tgaaagctat ctggtgtgcg aaagataaaa acaaagcgtt tgatgatgtg
     7681 atggcaggta aaagcgtcgc accagccagc tgcgacgtgg atattgccga ccattacgca
     7741 cttggcgtcc agcttggcgt tagcggtact ccggcagttg tgctgagcaa tggcacactt
     7801 gttccgggtt accagccgcc gaaagagatg aaagaattcc tcgacgaaca ccaaaaaatg
     7861 accagcggta aataattcgc gtgaaacaac agatacaact tcgtcgccgt gcagtcgatg
     7921 aaacggcaga cttgcccgct gaattgcctc ccttgctgcg ccgtttatac gccagccggg
     7981 gcgtgcgcag tgcgcaagaa ctggaacgca gtgttaaagg gatgctgccc tggcagcaac
     8041 tgagcggcgt cgaaaaggcc gttgagatcc tttacaacgc ctttcgcgaa ggaacgcgga
     8101 ttattgtggt cggtgatttc gacgccgacg gtgccaccag cacggctcta agcgtgctgg
     8161 cgatgcgctc gcttggttgc agcaatatcg actacctggt tccaaaccgt ttcgaagacg
     8221 gttacggctt aagcccggaa gtggtcgatc aggcccatgc ccgtggtgcg cagttaattg
     8281 tcacggtgga taacggtatt tcctcccatg cgggggttga acacgctcgc tcgttgggca
     8341 ttccggttat tgttaccgat caccatttgc cgggcgaaac attacccgcg gcggaagcga
     8401 tcattaaccc taacttgcgc gactgtaatt tcccgtcgaa atcactggca ggcgtgggtg
     8461 tggcgtttta tctgatgctg gcgctgcgca cctttttgcg cgatcagggc tggtttgatg
     8521 agcgcggcat cgcaattcct aacctggcag aactgctgga tctggtagcg ctggggacag
     8581 tggcggacgt cgtaccgctg gacgctaata atcgcattct gacctggcag gggatgagtc
     8641 gcatccgtgc cggaaagtgc cgtccgggga ttaaagcgct gcttgaagtg gcaaaccgtg
     8701 atgcacaaaa actcgccgcc agcgatttag gttttgcgct ggggccacgt ctcaatgctg
     8761 ccggacgact ggacgatatg tccgtcggtg tggcgctctt gctgtgcgac aacatcggcg
     8821 aagcgcgcgt gctggcaaat gaactcgatg cgctaaacca gacgcgaaaa gagatcgaac
     8881 aaggaatgca ggttgaagcc ctgaccctgt gcgagaaact ggagcgcagc cgtgacacgc
     8941 tacccggcgg gctggcaatg tatcaccccg aatggcatca gggggttgtc ggtattctgg
     9001 cttcccgtat taaagagcgt tttcaccgtc cggttatcgc ctttgcgccc gcgggtgatg
     9061 gtacgctgaa aggttctggt cgctccattc aggggctgca tatgcgtgat gcgctggagc
     9121 gattagacac actctaccct ggcatgatgc tgaagtttgg cggtcatgcg atggcggcgg
     9181 gtttgtcgct ggaagcggat aaattcgaac tctttcaaca acggtttggc gaactggtta
     9241 ctgagtggct ggacccttcg ctattgcaag gcgaagtggt gtcagacggt ccgttaagcc
     9301 cggccgaaat gaccatggaa gtggcgcagc tgctgcgcga tgctggcccg tgggggcaga
     9361 tgttcccgga gccgctgttt gacggtcatt tccggctgct gcaacagcgg ctggtgggcg
     9421 aacgtcattt gaaagtcatg gtcgaaccgg tcggcggcgg tccgctgctg gatggtattg
     9481 cttttaatgt cgataccgcc ctctggccgg ataacggcgt gcgcgaagtg caactggctt
     9541 acaagctcga tatcaacgag tttcgcggca accgcagcct gcaaattatc atcgacaata
     9601 tctggccaat ttagcgtcat cttctctata aaaaagagcg tggattgggt acaatcccgc
     9661 tcttatcacc gcattttgac tagctcaata aaagaaatca gaccatgttt gaaattaatc
     9721 cggtaaataa tcgcattcag gacctcacgg aacgctccga cgttcttagg gggtatcttt
     9781 gactacgacg ccaagaaaga gcgtctggaa gaagtaaacg ccgagctgga acagccggat
     9841 gtctggaatg aacccgaacg cgcacaggcg ctgggtaaag agcgttcctc cctcgaagcc
     9901 gttgtcgaca ccctcgacca aatgaaacag gggctggaag atgtttctgg tctgctggaa
     9961 ctggctgtag aagctgacga cgaagaaacc tttaacgaag ccgttgctga actcgacgcc
    10021 ctggaagaaa aactggcgca gcttgagttc cgccgtatgt tctctggcga atatgacagc
    10081 gccgactgct acctcgatat tcaggcgggg tctggcggta cggaagcaca ggactgggcg
    10141 agcatgcttg agcgtatgta tctgcgctgg gcagaatcgc gtggtttcaa aactgaaatc
    10201 atcgaagagt cggaaggtga agtggcgggt attaaatccg tgacgatcaa aatctccggc
    10261 gattacgctt acggctggct gcgtacagaa actggcgttc accgcctggt gcgtaagagc
    10321 ccgtttgact ccggcggtcg tcgccacacg tcgttcagct ctgcgtttgt ttatccggaa
    10381 gttgatgatg atattgatat cgaaatcaac ccggcggatc tgcgcattga cgtttatcgc
    10441 gcgtccggcg cgggcggtca gcacgttaac cgtaccgaat ctgcggtgcg tattacccac
    10501 atcccgaccg ggatcgtgac ccagtgccag aacgaccgtt cccagcacaa gaacaaagat
    10561 caggccatga agcagatgaa agcgaagctt tatgaactgg agatgcagaa gaaaaatgcc
    10621 gagaaacagg cgatggaaga taacaaatct gacatcggct ggggcagcca gattcgttct
    10681 tatgtccttg atgactcccg cattaaagat ctgcgtaccg gggtagaaac tcgcaacacg
    10741 caggccgtgc tggacggcag cctggatcaa tttatcgaag caagtttgaa agcagggtta
    10801 tgaggaacca acatgtctga acaacacgca cagggcgctg acgcggtagt cgatcttaac
    10861 aatgaactga aaacgcgtcg tgagaagctg gcgaacctgc gcgagcaggg gattgccttc
    10921 ccgaacgatt tccgtcgcga tcatacctct gaccaattgc acgcagaatt cgacggtaaa
    10981 gagaacgaag aactggaagc gctgaacatc gaagtcgccg ttgctggccg catgatgacc
    11041 cgccgtatta tgggtaaagc gtctttcgtt accctgcagg atgtgggtgg ccgcattcag
    11101 ctgtacgttg cccgtgacga tctgccggaa ggcgtttaca acgagcagtt caaaaaatgg
    11161 gacctcggcg acatcctcgg cgcgaaaggt aaactgttca aaaccaaaac cggcgaactg
    11221 tctatccact gtaccgagct gcgtctgctg accaaagcac tgcgtccgct gccggataaa
    11281 ttccacggct tgcaggatca ggaagcgcgc tatcgtcagc gttatctgga tctcatctct
    11341 aacgatgaat cccgtaacac ctttaaagta cgctcgcaga tcctctctgg tattcgccag
    11401 ttcatggtga accgcggctt tatggaagtt gaaacgccga tgatgcaggt gatcccaggc
    11461 ggtgccgctg cgcgtccgtt tatcacccac cataacgcgc tggatctcga catgtacctg
    11521 cgtatcgcgc cggaactgta cctcaagcgt ctggtggtcg gtggcttcga gcgtgtattc
    11581 gaaatcaacc gtaacttccg taacgaaggt atttccgtac gtcataaccc agagttcacc
    11641 atgatggaac tctacatggc ttacgcagat tacaaagatc tgatcgagct gaccgaatcg
    11701 ctgttccgta ctctggcaca ggatattctc ggtaagacgg aagtgaccta cggcgacgtg
    11761 acgctggact tcggtaaacc gttcgaaaaa ctgaccatgc gtgaagcgat caagaaatat
    11821 cgcccggaaa ccgacatggc ggatctggat aacttcgact ctgcgaaagc gattgctgaa
    11881 tctatcggta tccacgttga gaagagctgg ggtctgggcc gtatcgttac cgagatcttc
    11941 gaagaagtgg cagaagcaca tctgattcag ccgaccttca ttactgaata tccggcagaa
    12001 gtttctccgc tggcgcgtcg taacgacatt aacccggaaa tcacagaccg ctttgagttc
    12061 ttcatcggtg gtcgtgaaat cggtaacggc tttagcgagc tgaatgacgc ggaagatcag
    12121 gcgcaacgct tcctggatca ggttgccgcg aaagacgcag gtgacgacga agcgatgttc
    12181 tacgatgaag attacgttac cgcactggaa catggcttac cgccgacagc aggtctgggg
    12241 attggcatcg accgtatggt aatgctgttc accaacagcc ataccatccg cgacgttatt
    12301 ctgttcccgg cgatgcgtcc ggtaaaataa gcattacgtt atgctcacaa ccccggcaaa
    12361 tgtcggggtt ttttaattgt gctgcgcgaa agcagacaac aattttcttg cttcactatt
    12421 ggctgcctgc atcaccatcc acggactgaa cgcccacggt gtggcatcaa taccgtgtaa
    12481 tacatctgct aaatcacacc attgataatc catcacttca tcatcgttga tctgtaacgc
    12541 actggtggtg cgtgcggcaa ataccggaca cacttcattt tccacaatgc cattcggatc
    12601 ggtggcgcga tagcgaaagt caggatagat agattcagga ggcgtaattt ccacgccaag
    12661 ctcataacgg caacggcgga tcaccgcgtc ttcgttgctt tctcccagtt gtgggtgccc
    12721 acaaaccgag ttagtccaca cgccaggcca cgcttttttg ctcagtgcgc ggcgggtaac
    12781 taataattgt cctttggcat taaacagcca actggagaac gcgagatgta agcgggtgtc
    12841 tgccgtgtgt gcggcatact tttccagcgt acccgtggga actccctgtg cattcaataa
    12901 aatgacgtgt tccgtttgca taatttctca catgtaattc tgatcactca tcattataga
    12961 taattgaaga gtgaaatgat taaccaagtg agcgtttatc gacaaccgcc cgttttgagc
    13021 ggatgccgac aggtaaaaac catttaaaaa agtagcatca tatatttgaa gcgactttca
    13081 atggccattg caacacagtc agaaatgaca ggaatagaga gagtaaaaag caacagcaga
    13141 ctgaatataa cgcgcattaa aaaattcata ttattctcca tgctcatttt tcttcagctt
    13201 gcgcatcaga agccacatac gtactgtcct gacttttaaa tctttatcag agacggattc
    13261 cttcaccagg tcagcatgat gctggtagcc attaaagaag acattcagca caacggcgct
    13321 aagggttgcg agcataatgc cgctatgcag caacggttgc agtacggccg gtaacttaga
    13381 aaagaaatcg tgagagagcg tcggagtcat gccaacgccg agactgatcg ccacaatata
    13441 gaggttgtaa cggttggtgg tgtagttaca gcgcgacaga attcgaatcc ctgtcgccag
    13501 taccatgccg aacatcacca gcccagcacc gcccagcaca aattgcggaa tggaggcgac
    13561 cagcaccgcc atttttggca ccatgccaaa caggattaat ataattcccg aagaaataca
    13621 cacccagcgg ctatgaacgc gcgtcacgct aaccaggcca acgttttgtg aaaatgacgt
    13681 gtggaggaag ctgttaaacg ttccgcctat cattgtccct acgccatcga cacgcagccc
    13741 gcgaataata tcgtgtgaag agagtttacg accgactatt tcacccagtg ccaggaacat
    13801 ccccatcgac tcgataaaca cgatgattaa cacggcagtc atggtcagaa tggaaacggg
    13861 atcgaaaatc ggcataccaa atgacatcgg cgtgacaatc gcaaaccatg aagcatcatg
    13921 tagcccggat aaattgactt cattcatcat ccacgaaagt aaaaagccaa atacaatccc
    13981 cagtaatacg gcgacgttgg acataaatcc tttcgcatag cgagtaatga gcaagataaa
    14041 aattaagaca gcaaaggaga tacctaaata aacgggatta ccatattgcg gattcccttt
    14101 acctccggcg gcccagtcaa tacccacctg aatgatgcta agcccaatag aagtaataac
    14161 cacaccggta accagtggcg ggaataaagg catcaagcga ccaataaatg gcgctaataa
    14221 tgtggtgata aaacccgcgg cgatagtggc accaaatatc cccagcaggc cgatatccgg
    14281 gttcatccca atggctatca ttggtgttac agcagcaaag gtcaccgaca taatcaccgg
    14341 caggcggatc cccataaagc ggccgatacc gatacattgc aataatgtga cgatcccgca
    14401 gcaaaagaga tccgagctaa tgagcatcgc aatagcatct tttgagaggc ccagtcggtc
    14461 accaatcata agaggaacag cgactgcacc tgcgtacatg acaaggacgt gttgcagacc
    14521 gagtatgatc agctttcctg gtgataatat gcggtcaacc tcatcagttg ggcggtcttg
    14581 cccagaagat gagggaagtt gggaatctat ggcgctcatc agtgtttctc ctttcgcccg
    14641 ccagagtaaa aagcaaccgt ttacgttaat gaaactgtct aaacacatgc tcgtggagtg
    14701 ccagtttttg cgaggaaggg gagttagaga caggaattgc ggaggcaatc acataatcgt
    14761 gcttaaacca cgaaaatgac aagagaacct gacaatggaa ttatctgtaa ttttctcata
    14821 ttgatatttc attaatcatt ctggcatgtc gcactctcgc atttaatcgt ttttatctgg
    14881 atagcgctct tttgatcggc aatgttgaaa taatgaaagc ggcatagatt gaggggcaat
    14941 aatttgaact cagtcgcaaa attataatta aaacggtcat gctacctaat atatgcccgt
    15001 ttattgcatc gcgaaatttg agaagacata actca
//
LOCUS       DAZPAC010000096        14965 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZPAC010000096 DAZPAC010000000
VERSION     DAZPAC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.096"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHUPM_002436"
     CDS             <1..196
                     /locus_tag="ACHUPM_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870184.1"
                     /translation="KAILTDGLFALAQKKMPDATLRVLYSHICQIQQRIRFPQLAHFH
                     TCPPYQKFILKLLNNHFPAN"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="ACHUPM_002437"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="ACHUPM_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR0870185.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="ACHUPM_002438"
     CDS             complement(1608..1931)
                     /locus_tag="ACHUPM_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR0870186.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="ACHUPM_002439"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="ACHUPM_002439"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR0870187.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="ACHUPM_002440"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="ACHUPM_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR0870188.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="ACHUPM_002441"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="ACHUPM_002441"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR0870189.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="ACHUPM_002442"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="ACHUPM_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR0870190.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="ACHUPM_002443"
     CDS             7531..8568
                     /locus_tag="ACHUPM_002443"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0870191.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="ACHUPM_002444"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="ACHUPM_002444"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR0870192.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="ACHUPM_002445"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="ACHUPM_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR0870193.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="ACHUPM_002446"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="ACHUPM_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR0870194.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="ACHUPM_002447"
     CDS             10739..11620
                     /locus_tag="ACHUPM_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0870195.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="ACHUPM_002448"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="ACHUPM_002448"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR0870196.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="ACHUPM_002449"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="ACHUPM_002449"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR0870197.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14965)
                     /locus_tag="ACHUPM_002450"
     CDS             complement(14017..>14965)
                     /locus_tag="ACHUPM_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFR0870198.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     RCAMMRKESRGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3612 a         3785 c         3921 g         3647 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgccc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat tggcaacccg
    14941 actgaggtgt actggcaata gcgga
//
LOCUS       DAZPAC010000097        14722 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZPAC010000097 DAZPAC010000000
VERSION     DAZPAC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14722
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.097"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..1187)
                     /locus_tag="ACHUPM_002451"
     CDS             complement(<1..1187)
                     /locus_tag="ACHUPM_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0870199.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLG"
     gene            1836..2351
                     /locus_tag="ACHUPM_002452"
     CDS             1836..2351
                     /locus_tag="ACHUPM_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0870200.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            2569..3297
                     /gene="artP"
                     /locus_tag="ACHUPM_002453"
     CDS             2569..3297
                     /gene="artP"
                     /locus_tag="ACHUPM_002453"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR0870201.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            3315..4046
                     /gene="artI"
                     /locus_tag="ACHUPM_002454"
     CDS             3315..4046
                     /gene="artI"
                     /locus_tag="ACHUPM_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR0870202.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            4053..4769
                     /gene="artQ"
                     /locus_tag="ACHUPM_002455"
     CDS             4053..4769
                     /gene="artQ"
                     /locus_tag="ACHUPM_002455"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR0870203.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            4769..5437
                     /gene="artM"
                     /locus_tag="ACHUPM_002456"
     CDS             4769..5437
                     /gene="artM"
                     /locus_tag="ACHUPM_002456"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR0870204.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            5729..6460
                     /gene="artJ"
                     /locus_tag="ACHUPM_002457"
     CDS             5729..6460
                     /gene="artJ"
                     /locus_tag="ACHUPM_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR0870205.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(6635..7762)
                     /gene="rlmC"
                     /locus_tag="ACHUPM_002458"
     CDS             complement(6635..7762)
                     /gene="rlmC"
                     /locus_tag="ACHUPM_002458"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR0870206.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(7803..8291)
                     /locus_tag="ACHUPM_002459"
     CDS             complement(7803..8291)
                     /locus_tag="ACHUPM_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR0870207.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(8351..9196)
                     /gene="potI"
                     /locus_tag="ACHUPM_002460"
     CDS             complement(8351..9196)
                     /gene="potI"
                     /locus_tag="ACHUPM_002460"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR0870208.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(9193..10146)
                     /gene="potH"
                     /locus_tag="ACHUPM_002461"
     CDS             complement(9193..10146)
                     /gene="potH"
                     /locus_tag="ACHUPM_002461"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR0870209.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(10156..11289)
                     /gene="potG"
                     /locus_tag="ACHUPM_002462"
     CDS             complement(10156..11289)
                     /gene="potG"
                     /locus_tag="ACHUPM_002462"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR0870210.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(11384..12496)
                     /gene="potF"
                     /locus_tag="ACHUPM_002463"
     CDS             complement(11384..12496)
                     /gene="potF"
                     /locus_tag="ACHUPM_002463"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR0870211.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(12847..13323)
                     /locus_tag="ACHUPM_002464"
     CDS             complement(12847..13323)
                     /locus_tag="ACHUPM_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR0870212.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(13411..14313)
                     /gene="rimK"
                     /locus_tag="ACHUPM_002465"
     CDS             complement(13411..14313)
                     /gene="rimK"
                     /locus_tag="ACHUPM_002465"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR0870213.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(14374..>14722)
                     /gene="nfsA"
                     /locus_tag="ACHUPM_002466"
     CDS             complement(14374..>14722)
                     /gene="nfsA"
                     /locus_tag="ACHUPM_002466"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR0870214.1"
                     /translation="GVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPR
                     LPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPF
                     ILDYLHKQGWATR"
BASE COUNT         3833 a         3810 c         3473 g         3606 t
ORIGIN      
        1 tcacctaaac tgccgttttc aaaacaacat tttttcaata tgctttcatt cacaacagtg
       61 tgtgataagt agagattact tatgttttgc tttattaatg ccacgtcatt aaaatgacta
      121 tatttaatga aaaaaccatc attgagtagt gttttaagat tgactaaatt acttgcatct
      181 tcacgtacca tcgtggcaac tggaacaggc agagacatga aaacttcttc attcaccatc
      241 aagtgaatca tgcattgcgt tccctttgtc atatccacac atacgctaca aatatcgtgg
      301 taatgcgaag agcgcatttc aggaaacaga ttaaacacgg tatcttgaaa ggcactaaat
      361 cgtgttaata aatcctgata atgaacaggt tttgtttcaa aattatcatc agcaaatcca
      421 agcactattg tatgctctac atctaacgca gtttcaaatg gctcttcttc tgtaaaatca
      481 ttatttaaaa cctcacctga cgtattatca ctgtcataaa aagtgttcat caatgcgata
      541 ttgtttgtta cagcgtcttt ttcgatattc tcactaattg catatcgcct gctataatca
      601 taaaaatgaa taaacgcgcc ctttataaag gatatatatt gttctttgtt aaagtcaata
      661 cggctgtata atttttcaaa aatcacattc gcaattttac taccaatggt atgcctgtat
      721 aataatgggt gcgattcaat cgtaaaatgc ttgtctaatt gatcttgtaa tttattctca
      781 tagtccagag ggaaaagaaa ctgttgtctt tcaagtttcc cattcgatac attacgtatt
      841 ccaatagaga ttctttcatt acccgccttt atttcaactt cgttatcttc tgatttaccc
      901 agatattttt taacatcagt ctcgaaagca ttactaaaaa aagtattaaa ttggtaattc
      961 gctgatggca tattttccgc gagcaacaca tcatttttta tgagatcact gtcagcattt
     1021 attgctaata tcagatgcaa gtagatggta tcccatgtag acaactcacc cttacgactg
     1081 gaatttaata tcgcgaaaaa ttccctcagt gaatccagga aattatcact ttgatttagt
     1141 tgtgtgcctg gaaatgatga tgcttgatca gatatattgt ttatcatatt aaaaatcacc
     1201 caagaaaaac gataatccac cgaactcctc gtgctttata attataaaag cacaacgcct
     1261 tttatattca tattaattaa cgagatactg aaacaactta atttattctt cgctaaaatt
     1321 cattagctta aattctttag ccacaattgt agcataaata atattatcga gagtcaaagc
     1381 tgggactatc aaatttcgct cattttcaaa aatacaacgt aattttggca atcatcttaa
     1441 tcgcaggatc tcttatcgca tgtgaaaaat tttgtaatga ttagacaata accgcgttca
     1501 tttatcacgc gttataaatg tgaaactggc ttcttgcaga tgctcttaac ctctacagga
     1561 ggatttatac caccatttca gaactatcat atttacctta aatgatcatt tcagccatat
     1621 aacacgctca acctcacaaa tggtttacac tgtctattgt ttgggtaatt taagtaagtc
     1681 agctacgagc aaccttgagt ggatagccag caatggactc aaacccaatc aggtgttcag
     1741 attgtttcag aattgtatta aaaacaatgt attaaataat ctgatagcgt ttggtcgtag
     1801 tgtgacgcag acagcaagct aataaggaaa tgattatgcg ctaccgttca ctatctctac
     1861 ttattccatg tgcactgctg ctgagtgcct gtaccaccgt cacgccagct tataaagata
     1921 acggtccccg caccggttcc tgtgtccagg gggggcctga cagcgtggct caacaattct
     1981 atgactaccg cattcaacat cgcagcaatg acattaccgc cctgcgcccc tatttgagtg
     2041 acaaactggc aacccttctt agcgatgcca gccgcgataa tagtcatcgt gaattgttaa
     2101 gcagcgatcc gttctccagt cgaaccacct tgccagatag cgcccacgtt gccagtgcat
     2161 cgactatccc taatcgtgat gcccgtaaca ttccattgcg cgtagatctc aaacagggtg
     2221 accagggttg gcaggatgaa gtgttaatga ttcaggaagg ccagtgttgg gtcatcgacg
     2281 atgtgcgcta cttaggcggc agcgttcatg ccacggcggg tacgctccgt cagtccatcg
     2341 agaatcgcta acgccttgat caaccaataa accccgtaaa ttgtccggca tttcgtgagg
     2401 aatgccgaca tttatgctcg ccgaccaccg cccccgttat tttgtgctat gtttattgaa
     2461 taatgcgctt tgcttttaac ttttaaagca gaaatattgc ataattattc tgtcaaaggt
     2521 actatcttcg acctcaatag ctatcagact gccagtatac gagtgtcaat gagtattcaa
     2581 ttaaacggca ttaattgctt ctacggcgcg catcaggcgc tgttcgatat cacgctggat
     2641 tgcccacagg gcgaaacgct ggtgttactt ggccccagcg gcgcgggtaa aagctcgctg
     2701 ctgcgtgtac tcaatctgct tgagatgcca cgctccggta cgctcaacat tgcaggcaac
     2761 catttcgatt tcaccaaaac accctctgac aaagcgattc gcgatttgcg tcgtaacgtt
     2821 ggcatggtgt ttcagcaata caacctgtgg ccgcatctga ccgtgcagca aaacctgatt
     2881 gaagcgccct gccgtgtact ggggttgagt aaagatcagg cgctggcccg tgcagaaaaa
     2941 ctgctggaac gtctgcgtct caaaccttat agcgatcgtt acccgctgca tctttctggt
     3001 ggtcagcagc agcgtgttgc tattgcccgt gcgttgatga tggaaccgca ggtactgctg
     3061 ttcgatgaac cgaccgccgc actggacccg gaaattacgg cacaaatcgt cagcatcatt
     3121 cgtgagctgg cagaaacgaa tattacccag gtgatcgtca cccacgaagt tgaagtggcg
     3181 cgtaaaaccg ccagccgagt ggtgtatatg gaaaatggtc atatcgtaga acaaggcgac
     3241 gcgagctgct ttaccgagcc gcaaaccgaa gcatttaaaa actatctctc tcactaagat
     3301 tcgggataac aacaatgaaa aaagttctga ttgccgcgtt aattgcaggt tttagtcttt
     3361 ccgccacagc tgccgaaacc attcgttttg ctaccgaagc atcctatcct ccgtttgaat
     3421 cgattgatgc aaacaaccag atcgttggtt ttgacgtcga cctggcacaa gcgctgtgta
     3481 aagagattga tgcaacctgc actttctcta accaggcgtt tgacagcctg atcccaagcc
     3541 tgaaattccg tcgcgtagaa gccgtgatgg cgggcatgga tatcactccg gagcgtgaaa
     3601 agcaggtgct gtttaccacc ccgtactatg acaactctgc cctgtttgtg ggtcagcaag
     3661 gcaaatacac cagtgttgat cagctgaaag gcaaaaaagt cggcgtacag aacgggacga
     3721 cacaccagaa attcattatg gataagcacc cggaaatcac taccgttccg tatgacagct
     3781 accagaacgc aaaactggat ctgcaaaacg ggcgtatcga cggcgtcttc ggtgacaccg
     3841 cagtggtcac tgagtggctg aaagataacc cgaaactggc ggcggtgggc gacaaagtga
     3901 ccgataaaga ttacttcggc actggcctcg gcatcgcggt acgtcagggc aacactgagc
     3961 tgcagcagaa actcaacact gcgctggaaa aagtgaagaa agatggcact tacgaaacca
     4021 tctacaacaa atggttccag aagtaattcc tgatgaatga attttttcct ttagcaagcg
     4081 ccgccgggat gaccgtcggc cttgccgttt gtgcattgat tgtcgggctg gcgctggcga
     4141 tgttctttgc ggtatgggag tcggcaaaat ggcgtcctgt cgcgtgggca ggttcagcgc
     4201 tggtaaccat tctgcgtggc ctgccagaaa ttctggtggt gctgtttatc tattttggct
     4261 cctcgcagct gctgctgacg ctttcggatg gcttcactat caatcttggg ttcgtgcaga
     4321 tcccagtgca gatggacatt gagaacttcg acgttagccc gttcctttgt ggtgtcatcg
     4381 ctctgtcact gctgtatgcc gcctatgcct cgcaaacgct tcggggcgcg ttgaaagcgg
     4441 tgccggtggg tcagtgggag tctggtcagg cgctggggct gtcgaaatcg gctatctttt
     4501 tccgtctggt gatgccgcag atgtggcgtc atgcgctgcc tggcctcggt aaccagtggc
     4561 tggtgctgct gaaagatacc gcgctggtca gtttgattag tgtgaatgat ttaatgctgc
     4621 aaacaaaaag catcgctact cgtacccagg aaccatttac ctggtacatt gtggcggcgg
     4681 cgatttacct ggtgatcacc ctgctcagtc agtacattct caaacgcatt gacctgcgcg
     4741 cgacacgttt tgagcggagg cccagctaat gtttgagtat ttacccgaac tgatgaaagg
     4801 gctacacacc agcctgacgc taaccgttgc ctcgctgatt gtggcactga ttctggcatt
     4861 gatttttacc atcatcctga cgctgaaaac gccggtgctg gtgtggctgg tgcggggtta
     4921 tatcacgctg tttaccggta cgccgctgct ggtgcagatc ttcctgattt attacgggcc
     4981 gggccagttt ccgacgttgc aggagtatcc ggcactgtgg catttgttgt cagaaccgtg
     5041 gttatgtgcg ctgattgcgt tatcgctgaa cagtgcggcg tataccacgc agctgtttta
     5101 cggtgcaatt cgtgcgatcc cagaaggtca gtggcagtcc tgtagcgccc tgggaatgag
     5161 caaaaaagat acgctggcgc tcctgctgcc gtatgccttt aaacgctcgc tctcttctta
     5221 ttccaacgaa gtggtgctgg tgttcaaaag tacctctctg gcttacacca ttacgctgat
     5281 ggaagtgatg ggatacagcc agttgttgta cggacgcacc tacgatgtaa tggtgttcgg
     5341 tgcggcaggg attatttacc tggtcgtcaa cggcctgctg acgctgatga tgcgtctgat
     5401 cgagcgcaaa gcactggcat tcgaacggcg gaattaatgt agctgcgtac attcgccggg
     5461 ggggtttgta ggtctgataa gacgcgccag cgtcgcatca gacatcagca cggtgcctgt
     5521 gccggatgcg gcgtggacgc cttatccggc ctacatagtg cataaattct tataatgaag
     5581 acggacaact cactaagtta tccgtctttt tttatttcat ttaaattatt taatcatatt
     5641 tattgcatat aaattcactt gatggcattg ttatcccatg ccgcagacac ggccaaaaat
     5701 cataagattg acagacggga gttccatcat gaaaaagtta gttcttgccg ctttacttgc
     5761 ttcctttact ttcggtgctt ctgccgcaga gaaaatcaat tttggcgttt cagccaccta
     5821 tccacccttt gaatctatag gtgctaataa tgagattgtc ggctttgata tcgatctggc
     5881 aaaagccttg tgcaaacaaa tgcaggcaga atgtactttt actaatcacg cgttcgacag
     5941 cctgatcccg tcactgaaat tcagaaaata tgactccgta atctccggta tggatatcac
     6001 gcccgagcgt agcaagcagg tatcgtttac cacgccctac tatgaaaact cagccgtcgt
     6061 gattgccaaa aaagatacct acaaaacgtt tgccgatctg aaaggcaaac gtattgggat
     6121 ggaaaacggc accacgcacc agaaatatat tcaggatcag caccccgaag tgaaaactgt
     6181 ctcctatgac agttatcaga atgcctttat cgatctgaaa aatggtcgta ttgatggggt
     6241 atttggtgac acagcggtgg taaacgaatg gctgaaaacc aatccgcaac tgggcgttgc
     6301 tactgaaaaa gtgaccgatc cgcaatactt cggcaccggt ctgggcatcg ctgtacgtcc
     6361 ggataacaaa gctctgctgg aaaaactgaa taacgcgctg gcagcaatta aagctgacgg
     6421 tacatatcag aaaatcaatg accagtggtt cccgcagtaa ttgagactga tgacaaaagt
     6481 aagattgcct gatgcgctgc gcttatctgg cccacatcaa ctctgcaatg tattgattta
     6541 gcatgatttt gtaggccgga taaggcgtac acgccgcatc cggcatgaac aaagcgcacc
     6601 tgatcagcaa tctgaagccg cacctgcggc ttttttattg cttcaccagc agcgtcatca
     6661 cttcatagtg cgcggtgtgc gggaacatat caaaaagctg tacccgttcg atgcggtaac
     6721 ctggcagttc gcggatatct ttcgccatgg tttgggcgtt acagctggag tagataataa
     6781 aacgcggtgc catcgttgag agataatcac acagcggttt accaatgccg cggcgcggcg
     6841 ggttaaccag caccagctcc ggcacatccc cctgagcggt ggcaaactga gtggagtcca
     6901 gcgcctgaaa ttgcaaacgc gttaagccca gttcagcggc tgactgcttt gcacaggcaa
     6961 tggcctctga tgcaatttcg atcccggtta actgcatgtc aggcgtcgcg cagtgtaaac
     7021 caaagccacc cacaccgcag aacagatccc acatatgttt aactggcagc tgccgtaccc
     7081 agtcgcgcgc ggtagcgtac agctggctgg cgaccgccgg attagtctgg aagaaacttt
     7141 gcggacggat ccacagcggt acgtcattaa aacgctctgc cagcgcctgt tgttcagtca
     7201 ggtagatctc cgtctcccct tccataatcg ccatatgtac cggctgaata ttgacggtaa
     7261 taactttcag ttgcggtagt tgttcctgta accacggcag cgccttacgc agttgtgcca
     7321 gtttggtttc agaacgcagt acaaagcgca gcatcatgcc gccatcgctc tggctttcag
     7381 tcagcagaat gtatttcagt tcgccacgct tacgcgccac gttgtagggc gttaaccccg
     7441 cacgggcgat aaacggtttt agcgccgcaa aaacgggcgc aaaagaggca ggataaagcg
     7501 ggcagtcaca aaggtcttcc ggcgtgccat cgcgatgcag cataccgagc agtggttttt
     7561 caacgctacc actcaccacc attttggctt tattacgaaa cccttgttcc gggcctgaca
     7621 ccggcgcgca ccattcctca accggaaaat cggcgagcag atttttaaga tcggcggttt
     7681 tagcggagag ttgctctgga atcggctgcg ttatccactg acaggaacga cagcgacccg
     7741 cgtcgtaaag tgcgcactgc atacattgac cttcacatca tcaggggcga cgattataca
     7801 cattattgca actggaagaa ccgccgactg gtcgagggaa cgaacagcag catcaggatg
     7861 agcatatccg gcagcttctg cagcatcagg ctatggaaga tttcacgttt tgattcaccg
     7921 ggaatgctga acagctccgg ataaccgtac cccagcgaag ccgcccacaa gtaacttgcg
     7981 gcggtgattt gtgtcagcag atatagccag cgcgcccagc gacgcccttt caccagtgaa
     8041 aacgcacacc agatctcaat gaaaaccagc accagactgc ttaaaaagac cagcgttaaa
     8101 ctccaggttt gtacgctgcg atgaatgaac tcgccaatac cgcgcacgcc cagcgtattg
     8161 aaaatcatca gcacgtcgag gccacggatc ataataatgg cgagcgccgc cacctgcacc
     8221 agcgcaggca cattcaggcg agcatgagat gaagatgttt tcttaaaaaa tcccaacgtt
     8281 tcgtcttcca tgaaaacaat gccgcggcat gcgcggcatt atgtagccag gttggcaaat
     8341 tttagtgtct tcagccatgt cttgcacgct ggatatcgcg tatccgctgt ttttctgcgc
     8401 gagccatcag ataccaggcg ataaatccga caattccgac cgcaccgaga attaacgttg
     8461 caagggcgtt gatttccgga ttcactccca tccgcacgct ggaaaagacc agcatcggta
     8521 acgtggtggc tcccggcccg gaaacaaagc tggcgatcac cagatcatca agcgacaaag
     8581 taaaagccag taaccagcca gaaatgatcg cgggcatgat catcggtagc gtaatgacga
     8641 aaaacacttt cagcggcgtc gcaccgagat ccatcgctgc ttcttctatc gagcgatcca
     8701 gttcccgcaa tcttgaagaa ataacgaccg ccacataagc cgtacaaaaa gtgacatgcg
     8761 ccagccagat ggtgagcata ccgcggtccg caggccagcc aatggcatga gcaagcgcga
     8821 cgaataataa caacagcgac aagcccgtga tgccatctgg catcaccagc ggcgcggtga
     8881 tcataaaggc aaaaccattt gatccgcgaa acctgccaaa acgcaccagc accaccgccg
     8941 caatagtccc gaggatcgcc gccgccgttg ccgcacaggc agcaattgtc aggcttaaac
     9001 caaccgcact catcatcgcg tcatcgcgca ataactcacc ataccagcgc gttgaccagc
     9061 cggcccacac cgtcaccagc ttcgagctgt taaacgaata gataaccagc atcagcattg
     9121 gcgcgtagag aaaggtaaag cccagcagca aaatcacaat ccgccagggc gaacgaacta
     9181 ccggtaaatt attcatccgt gttctcccac gcttttttgc tggtgtttgt ggaaccacat
     9241 tatcggcaca attagcagca gcaacatgat gatcgctacc gccgaggcca ccggccagtc
     9301 gcggttattg aaaaactctt gccataacac gcgcccgatc atgatgctgt ccgggccacc
     9361 gagcagttcc gggatcacaa actcgcccac agccgggata aacaccagca tcgatccggc
     9421 aataatccca cctttagtca gcggcacgat cacagtaaag aacgttttca gcggtcgtgc
     9481 accgagatcc agcgctgctt ccaccagcga ataatcaata cgaatcaacg cggtataaat
     9541 cggcagtacc ataaacggca cgtaagcgta aacaatgccg atataaacgg ccagattggt
     9601 atgcagaatg gtcagcggtt gatcgataac ccccagccac agcagaaaat tattcagcac
     9661 accgttgttt tttaatattc ccatccaggc ataaacgcgg atcagaaacg aggtccacga
     9721 cggcaggatc accagtagta ataaaatatt acgggtcgaa ggcttactgt gcgccaccgc
     9781 ccacgccagc ggatagccga tcagtaaaca gcaaaatgtc gaaatcgccg ccacctgtaa
     9841 cgactggaga taagcatcaa aatagagcgg atcgtcggtc agttgcagaa aattaccgag
     9901 attaagagtg atggaaagtt gcccgtcagc ccactccatc agttcggtat aaggtggaat
     9961 agcgcgcgcc atctctccca ggcttatttt aaagacaatc agaaatggca gcagaaacag
    10021 caagatcaac cagatatata gcaacgcaat gaccagtttg cgcccatgct tcatttgcag
    10081 ctgcgacaac cacagcttaa atccgcccgg cggtttcgac tgggcagcag gttcaagtgt
    10141 actcatcttt gctccttaaa ccgtcagcac cacacagctg tccacttccc agcacaaacg
    10201 cacttcgtcg ccccaggtcg gtaacccttt acgatgacga tgggcgtttt gtagctgggc
    10261 gctgatcatc tgcccacttt tcagtcgaac gtgatacacc gaaagatcgc cgagataggc
    10321 aatgtgtatc acctccccca ccgcgaagtt acaaccattg gcgggcggct cttcgcaaag
    10381 catgattttt tccgggcgca gcgctacgtg taccggcacg ttatcgacca ctgaagcatc
    10441 cgcatcgact ttcagtggat gcaccagccc cggcgaatca agcaccaggc catcttcctg
    10501 acgctctttg agtacacctt caaagacatt aaccgaaccg ataaattcag cgctgtagcg
    10561 ggtagttgga tgctcgtaga tctcttccgg ctcgccaatc tggacaaatt tcccgcgatt
    10621 cataatggcg atacgccccg ccatggtcat cgcctcttcc tgatcgtggg tgaccatcac
    10681 acaagtcaca ccgacgcgct ccagaatatc caccacttca agctgcatcc ggtcacgcag
    10741 ctttttatcc agcgcgccca tcggctcatc gagcagtaat agtttcgggc gtttggcaag
    10801 gcttcgggcc agggccacac gctgtcgctg accaccggaa agctgatgcg gtttgcgttt
    10861 ggcgaactcc tgcatgtgca ccaggccgag catctcatgg acccgactgg caatttccgc
    10921 tttcggtagt ttgtcctgtt tcaggccaaa agcgatgttc tgttccacgg tcatatgcgg
    10981 aaacagcgcg taagactgaa acatcatatt gatggggcgc aggtaaggcg gcacctgtga
    11041 caaatcgacg ccatcgagca ttatctgccc ggcagaaggt tgttcaaaac ctgccagcat
    11101 acgcagcagc gtggacttgc cacagccgga tgcgcccagc agcgcgaaga tttcaccttt
    11161 gtagatggtc aggctgacat catcgaccgc atgctgacca tcgtaggatt tggtgaggtt
    11221 gcggatttct aatagcggcg tcagcgcctt acgggttttc gcctgcgggc gagggatagc
    11281 gtcattcacg gctgctctcc ggcattgaat gtgtgcaagc gcaccatgat ggtgcgaatt
    11341 gccggctggt gtggtgcgcc cgcccggcat ctaaggctgc ggattatttt ccgctcttca
    11401 ctttggtcca cgcgcgggtg cgcacacggt cgattttcgg atcctgtact ttcagagtga
    11461 atagcttcgc acgaacgtcc gccggaggat aaatacccgg gttctcacgg acttccgcac
    11521 tcaccagcgg cgtggctgct ttattggcgt tggcatagaa cacatggtcg gaaatatgcg
    11581 ctactacatc cgggcgcagc aggtaattca ggaactgata ggcttcgtct ttgtttttgg
    11641 catccgcagg catggcgaat acatcaaaga acgccatcgc cccttctttt ggaatcgaga
    11701 acgagacatt cactccattc ttcgcttcct tcgcgcggtt tgacgcctgc cagacatcac
    11761 ctgcccagcc gatagcgacg caaatatcgc cgtttgccag gtcgttaatg tattgagatg
    11821 aatggaaata acgaatgttc gggcgcagct ttaacagcag atcggttgcc ggtccggtgt
    11881 aatcatccgc tttagtgctg ttgggatctt tgccgagata attcaacacg gtagcaaaaa
    11941 cttcttctgg cgcatccagg aaagagacac cgcagctttt cagtttttcc agattttcag
    12001 gtttgaggat caagtcccag ctatcgacgg gcgcgttttc gcccagcaca gctttaactt
    12061 tatcaacgtt atagccaatc ccggtcgtcg cccacatata gggcatagca aatttattgt
    12121 cgggatcgtg tttggcgacc agcttcagca gctccgggtc gagattcttc cactccggca
    12181 atttgctttt gtccagcggc tggaaaactc ccgcagtcaa ctggcgttca agaaagctgg
    12241 cagatggaac caccaagtca aagccggtgc tacccgccat taatttgcct tccagtactt
    12301 cgttagagtc gaaaacatcg tagacgactt taataccggt ttctttttca aaattggcca
    12361 ccgtgtccgg ggcgatataa tcagaccagt tataaatgtg gagtgttttt tgttcagccg
    12421 cgagcgtgcc gacagagacg gccatcagag cacccgcagc cagacccgaa agccattttt
    12481 tatttaaggc ggtcatatct ctttccttct gaaagttcgt taacaaacga acaaaagttg
    12541 tcacactaaa agatatgcaa taaacgtgca tattttttat cggcggggtg taagaaaaga
    12601 gaactctctc ccggccagca ctgctcgcta tataaaagca tcgcaagaat aactttagcc
    12661 agggtttgag actatagctc ggattaaaaa acggtattta acaaattttt atgcgtattt
    12721 gctctatgtg ataaagccaa atcgacacaa gagaagtgaa ataatcttta agaaaaggtt
    12781 aaaaacagca gataattttg cagcgcgcag ccactgcacc agcgactgcg tgctcgaaag
    12841 gagttatcag tggaggaaat agttttcggt aacgttgttt tgcccttcat cgtcagtcgg
    12901 taacagcagt tgatgggcat tagcttcaag aatgaccatc gaaatctgct cttcgctctg
    12961 acgcacgaac catgcaaact gctcataggt tacgccctgc ataacggata aagactggca
    13021 aaccaccagc tttggcagat tatcatcctg catatcaaga aatgctttca cggtcagcga
    13081 actggcattg atggcagata aatccgccgc cagcggcaat acggctgaag gtcggacttc
    13141 cgccatggca gaaaacagga tcgtgttatc gatcagatcg attttggcat caaagacacc
    13201 gtcgaaattc tgcatatggg gcagatgcag agcctgacag ttatcacatt caaaaaaact
    13261 catccccagg tcatcgagcc attgacgcag cgtatccaga ccaggaacga ccagcgatgt
    13321 cataattttg tacaacctct ttcgataaaa agaccggcac agcttacgca aaaagcgcag
    13381 gcaaaaccat gatcagtaat gtgattgcga ctaaccaccc gttttcaggc aatattctgt
    13441 cgtagcgtgg cgttcgatcc agcggatcat tttacccgcg atgtcgatac cagtggtttt
    13501 ttctattcct tccagccccg gcgacgcatt cacttccatc accaacggcc cgcgattagc
    13561 acgcagaata tccacaccag caacgtccag cgccatcgtt cgcgcggctt ttatcgcgat
    13621 ttcacgctcc tgtggtgtga tactggcgac gcttgccgcg ccgccacgat gcaaattgga
    13681 acgaaaatcg ccctctttcg cccgccgttc aatcgcagcg accacttcat cgccaacaac
    13741 cagacagcgg atatcgcacc cttgcgcctc tttgatatat tcctgcacca gaatatgcgc
    13801 gttcagaccg cggaaagcgt caatcacgct ttccgccgcc tgacgcgtct ccgccagcac
    13861 gacgccaatt ccctgcgtgc cttcaaccaa cttgaccacc agcggcgcac caccgaccat
    13921 gtcgattaaa tcgctggtat catccggcga atgcgcaatg cccgtgacag gcaggtcgat
    13981 gccctgacgc gccagcagtt gcatggaacg caatttgtca cgcgcccggg caatggcgac
    14041 cgactcattg agcggatagc tccccagcat ctcgaactgg cgcagtgccg ccgtcccata
    14101 aaaggtaatg gcggtgccaa tacgcgggat cactgcgtca aaatggggta acttgcggcc
    14161 tttgtagtga atagaagacg ccgcaggatt tatgttcatg tagcaagaaa gcggatcaag
    14221 aatttcaacc aggtgaccgc gctgtatagc ggcttcacgc agccgcttac acgaatagag
    14281 cgttccatcc cgggacaata tggcaatttt caccctgcac ctctctgtca gacctggtca
    14341 aaagcccgcg tatcatacat cgacgtggcg gttttagcgc gtcgcccaac cctgtttgtg
    14401 caaataatcc agaataaatg ggcggctttc tttaatgatt gttcggcgga tatgatcgct
    14461 ccaggtatcc cggcgattat tgctgccacg ggtgaggtaa tattccgcca gttgctcgtc
    14521 atactgcgcc agtgcgtctt tatccagcgg ttgatagctg ttttcatgca ccaaaatgga
    14581 ggccggtaaa cgcggcttga gatccggatt atccgcaggc cagccaaggc acagcccaaa
    14641 cagcggcaga acatgctgcg gtaatttcag cagtttcgtc accgcttcaa tattattgcg
    14701 caggccgccg atatataccc cg
//
LOCUS       DAZPAC010000098        14597 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZPAC010000098 DAZPAC010000000
VERSION     DAZPAC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.098"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(16..2001)
                     /gene="yjcS"
                     /locus_tag="ACHUPM_002467"
     CDS             complement(16..2001)
                     /gene="yjcS"
                     /locus_tag="ACHUPM_002467"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFR0870215.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            2254..2571
                     /locus_tag="ACHUPM_002468"
     CDS             2254..2571
                     /locus_tag="ACHUPM_002468"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR0870216.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            2640..2969
                     /gene="yjdP"
                     /locus_tag="ACHUPM_002469"
     CDS             2640..2969
                     /gene="yjdP"
                     /locus_tag="ACHUPM_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFR0870217.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(3116..3874)
                     /gene="phnP"
                     /locus_tag="ACHUPM_002470"
     CDS             complement(3116..3874)
                     /gene="phnP"
                     /locus_tag="ACHUPM_002470"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFR0870218.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(3876..4310)
                     /gene="phnO"
                     /locus_tag="ACHUPM_002471"
     CDS             complement(3876..4310)
                     /gene="phnO"
                     /locus_tag="ACHUPM_002471"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFR0870219.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(4297..4854)
                     /gene="phnN"
                     /locus_tag="ACHUPM_002472"
     CDS             complement(4297..4854)
                     /gene="phnN"
                     /locus_tag="ACHUPM_002472"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFR0870220.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(4854..5990)
                     /gene="phnM"
                     /locus_tag="ACHUPM_002473"
     CDS             complement(4854..5990)
                     /gene="phnM"
                     /locus_tag="ACHUPM_002473"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFR0870221.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(5987..6667)
                     /gene="phnL"
                     /locus_tag="ACHUPM_002474"
     CDS             complement(5987..6667)
                     /gene="phnL"
                     /locus_tag="ACHUPM_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFR0870222.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            complement(6778..7536)
                     /gene="phnK"
                     /locus_tag="ACHUPM_002475"
     CDS             complement(6778..7536)
                     /gene="phnK"
                     /locus_tag="ACHUPM_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFR0870223.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(7533..8378)
                     /gene="phnJ"
                     /locus_tag="ACHUPM_002476"
     CDS             complement(7533..8378)
                     /gene="phnJ"
                     /locus_tag="ACHUPM_002476"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFR0870224.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(8371..9435)
                     /gene="phnI"
                     /locus_tag="ACHUPM_002477"
     CDS             complement(8371..9435)
                     /gene="phnI"
                     /locus_tag="ACHUPM_002477"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFR0870225.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(9435..10019)
                     /gene="phnH"
                     /locus_tag="ACHUPM_002478"
     CDS             complement(9435..10019)
                     /gene="phnH"
                     /locus_tag="ACHUPM_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFR0870226.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(10016..10468)
                     /gene="phnG"
                     /locus_tag="ACHUPM_002479"
     CDS             complement(10016..10468)
                     /gene="phnG"
                     /locus_tag="ACHUPM_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFR0870227.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(10469..11194)
                     /gene="phnF"
                     /locus_tag="ACHUPM_002480"
     CDS             complement(10469..11194)
                     /gene="phnF"
                     /locus_tag="ACHUPM_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFR0870228.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(11215..11994)
                     /gene="phnE"
                     /locus_tag="ACHUPM_002481"
     CDS             complement(11215..11994)
                     /gene="phnE"
                     /locus_tag="ACHUPM_002481"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFR0870229.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(12120..13136)
                     /gene="phnD"
                     /locus_tag="ACHUPM_002482"
     CDS             complement(12120..13136)
                     /gene="phnD"
                     /locus_tag="ACHUPM_002482"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0870230.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(13161..13949)
                     /gene="phnC"
                     /locus_tag="ACHUPM_002483"
     CDS             complement(13161..13949)
                     /gene="phnC"
                     /locus_tag="ACHUPM_002483"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR0870231.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(14082..14525)
                     /gene="yjdN"
                     /locus_tag="ACHUPM_002484"
                     /pseudo
     CDS             complement(14082..14525)
                     /gene="yjdN"
                     /locus_tag="ACHUPM_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
BASE COUNT         2940 a         4051 c         4346 g         3260 t
ORIGIN      
        1 caaaagcgag tgacattaat ttggggttac gatattcacc cacaaatcga aattatccag
       61 acaggcgata atttcttcca gtttcgcgcc attgccaata attttggctt tcttcgcttt
      121 taccagatcc gccattttgg cttgcccggt cagcacggcg tgcagatctt cacggctgat
      181 gtagaaagag gcgtcagctt gggattgcag tgttttacgg tagttaagca cgctatcgtt
      241 cagcgtcagg ttgaggttat cgccattgct catattgaag ttcaggctga tatttttacc
      301 cgcggctttc gcgctatcga ggcgaacgga cataaagtcg aacagcattt cgaccgacat
      361 cccgcgaatg gtgtccgggg aaccgatggt gccgtggctg aacttatgca ccccttcgcg
      421 cagctctttc gcgccggtca ggtagaaacc gcgccaggtg gcggactcgg cctgatagcc
      481 cagctgttca aagttattcg cttgcagatt cttcgcgacc tggtcacccg ggttggcggc
      541 aatcacctgt ttcagcagtt ctgccgacca gcggtaatca ccttgcttgt tcgcttcttg
      601 cgccaggttg atgacacggg cagaaccgcc cagcgcctgc acgtaacgtt tacccatctc
      661 cacctgacca tacggatgca ggttagccgg gttaccgtcg taatagccaa gatagaagtt
      721 atacaccgcg cgggcgttgt ggctgacaga accgtaatag ccacggctgg cccagttatt
      781 ggcaagtgca ggcggcagtt taatcatgtc gccgatttca ttcatggtgt agccctggtt
      841 cgccaggtgc agggtctggt cgtgaatgta cttgatagta tcgcgatatt taccaatata
      901 atcattgata tgcttattgc cccagaccgg ccaggtgtgc ggcataaaca ggacttccgc
      961 gtcgttaccc cacatatcca gcgtttcgtt cagatactcg gtccacttgc tggtgtcgcg
     1021 ggttttcgcg ccgcgcagag tgtagaagtt gtgcagggta tgcgtggcgt tctcggcggt
     1081 acacagcgct ttcagggccg gaatatagaa gtgcatttcg gctggtgctt cgctacctgg
     1141 ggtcatcagg aagtcaaact ccaggccgtc gataatcatc ttctcaccgg ttttggtgat
     1201 ggttttgttc ggtgcgatga tgctcgggta gcccgatgcc agcgttacgc caagaccatt
     1261 gccgatgtta ccctgagggt tatgcgccag cagcaggccg taggagtaaa gtgcacggcg
     1321 gctcataata ttacccgcca gtacgttttc gctgatggct tcgtccataa agccagcagg
     1381 ggcgataacc tgcactttac cggatttcac atcagcttca gagataatgc ctttcacgcc
     1441 gccatagtgg tcggtatggc tgtgggtata aataaccgca acaatcggtt tttgcgggcg
     1501 attttggaag taaaggtcaa gggcggcttt tgcgctcggt ggcgtcacca gcgggtcgat
     1561 gacaataatc ccggtatcac cttcgataaa ggtgatgttg gagatatcct gaccgcgaac
     1621 ctggtacatt ctgtcggtga ctttaaacag gccggaaata ccgtttaact gcgactgacg
     1681 ccacaggcta gggttaacgg tttcgggcgc gggcgcattg atatcaaatt tataatcttc
     1741 cccgcgataa tatggtttgc cattagcatc gcgcagaata ccttcatcca gcagcggagc
     1801 gataaatcca cgctgagcgt cgtcaaaatc ctgacgatcg ctaaacggta atgatttggc
     1861 gtaattctga ttaacctgtt gggtatattg agtggcggct ttcgcttcct ctttcgcgtt
     1921 agcggtattt accatcatgc cgctggcggc agttaacgca ataacaattc tgctcaaacg
     1981 gaataaccga gagttattca tgtaaatctc catttatcat ttatgtcgta aatatgtaat
     2041 ccatgcaaaa tacgtcattt aaagctgggg ttatcataat tgggaatttg cgactttaat
     2101 aagtggaagt gtgagctgaa cgatccattt tattaggcat ttgtgctgaa gagggaaaat
     2161 tacgcgaatt tattatctct gtaaataaca tgaggtgttt tatatattta ttatatatat
     2221 tgtacttcgg caaatactga gcacaattta tcaatgcaac cggtgtttct ttttaaaaaa
     2281 cgtaccggag caatgggcgg tcatgtcgct ggcaccgcag gattctgcgt tgctgtgcca
     2341 gccttttatt catggtcaat gagtgaggtg attgataaag acaccaggtc gtcagagcgc
     2401 actgattatc cgcattatgc cagtgaagtt gcactggctt ttggttcacg agtggttggc
     2461 ctcgaactgg caaaaatgat tgtggatgcg tggctgggcg cacagtacga aggcggtcgt
     2521 catcaacaac gcgtggaggc gattacggca atagagcagc ggagaaattg agattcatcc
     2581 actacttgca tggatgagta atgactaatg tggatagagt ttctttttga ggttggctaa
     2641 tgaaacgctt tccgctattt cttttattca ccctgctcac gctgtccacc gttccggccc
     2701 aggctgatat tatcgacgat actattggta atattcagca ggcgattaat gacgcttata
     2761 accctgaccg tgggcgagac tatgaagatt cgcgcgacga cggctggcag cgtgaagtga
     2821 gcgacgatcg gcgcagacaa tatgacgacc gccgccgcca gtttgaagat cgccgcaggc
     2881 agctggacga tcgccagcgc cagcttgatc aggagcgtcg gcaactggag gatgaagagc
     2941 ggagaatgga agatgagtat gggcgatgag gttgggtatg gagcaggcat cgcccctcac
     3001 cccaatgggg agagggagaa aacgagcgca atattcaata ttgagcacaa tcggtcccct
     3061 cgcccctctg gggagagggt tagggtgagg gggccagcgc gcacattccc tctcatcacg
     3121 ccaccccaat ctccatccca tcaaacccca cctcaaaccc tgacggtagt gcgttttcca
     3181 tcagccacgc atcaaactgg tggctgatat gggtcagaat cacccgtggc gagcggataa
     3241 cctgattcag cgcaagcacg gtatttaaat cacagtgatt acgcggtgca tccgcgcgcg
     3301 gcgggtgact gcaatccatc accattacct gcggctgatt attgcgtaaa aatttcagcg
     3361 ttttttccgg caagccagcg gtgtcagaca gccacgccac ccggctgtgc gccgtttcca
     3421 gtaaatagcc gaaagtgagt tttgagtggt taagcggcag cggcgtgacc tgcaacccct
     3481 gcaaatcaaa caccacaaac ggttccaccg tgtggctgaa atcaagcagc ccgggatgtt
     3541 taaacagatc gtcgcagccc tgttcatccg gcgggccgta gatcgggatc ggatcgccca
     3601 cgccccagcg cagcggaaac agcccctgga cgtgatccat atgataatgc gtcagcaaaa
     3661 actgctggaa cgatccggga gaccagcgat cggtgagatc gtgcagcccg gcgtcgatca
     3721 gggtgatcgc ctcgttaaac ttcactacgc cgctgcacgg ttggcggcga tactgcggcg
     3781 agcgccgcgc tcggtcgcag gccgcgcact cgcagcccca tgccggaacg ccctgtgcgc
     3841 cgccggtgcc ggtgagcgtg agggtcagac tcatgttaca gcgccttggt gaagcggaag
     3901 tgactctgct cgtagccttc tcgcagatag aaacggtgcg cgtcgtggcg cttcacgttg
     3961 gtcgaaagct cggtcatttc ggccccggcc tggcgggctt cttcttctgc ccacgccagc
     4021 aacttgctac cgacgttcag gccgcgcgcc tgcggcatca ctaccagttc ctgaatttcg
     4081 ccgatccagt tgacatgatg cagatgaaac tgcaaatgca ggccgatcat gccgacaacc
     4141 tcgccatcca gcagcgccag atgatagcgc atgttcgggt cgcgcagatt ggcgctaaag
     4201 cccacgcgaa atgcgtggtg gtcaaactcc gcctgtttta gctcacaaat cagcgcgtaa
     4261 accgcgtcgg tatcatactg cgtggccggg cgaagctcac aagcaggcat ggtgtttctc
     4321 cttctgatga atcagcgtca gcagcgtgtc gaccgactgg cgcaggctgc cgtcattgtt
     4381 gagcgtatgg caatcctgtg gagtatagcg ggcggcgcgc gccaggcggg cgttaatttc
     4441 actggcgttt tcacggccac ggttttccag gcgctggcgg aggatctccg gcgaaacctg
     4501 taaacagacg ggcagcagcg ccgattgata gcgcgcccgc gcctgcggca gatgggcgcg
     4561 tgagccgttg accagcacgt cgaatccggc gtgcagccag aggtcgatct cgacgccgac
     4621 gccataatac agaccgttag cgtgccagct taaggccagt aggttctgcc ccgcgcgggt
     4681 aaaaaactcc tgctcgctca gagcgatatg gttttcgctt ccggcgctgg cgtcgcgcgt
     4741 gatgtagcga tgcgccacca gtagctgagt ttgttcccgc aggcggagtt ccgccagcag
     4801 gctatctttc ccggagccgg acggccccat taaccaaatc agttttccca tcatcagaac
     4861 acccttttac cctgacgcca gacgtggtcg atatgaatgt gattgccctt gcgatgcgcc
     4921 agcaccaagt ccgcgcgttt gccctcgcca atcaccccgc gatcctggag attaagcgcc
     4981 tgcgctggat ttttagtcac cagcttcacc gcctgcggca gcgtaaagcg gttgctctcg
     5041 tcatcggcga cgcgaaatgc cgcatcgagc aggctggcgg ggtagtagtc ggaagagagg
     5101 atatccagca ggccaagctg cgccagttca ctggccgcca cgttgccgga gtgcgagccg
     5161 ccgcgcacaa tattcggcgc gcccatcagc acgttcatgc catgcttgcg cgaggcttcc
     5221 gccgcttcga acgtggtggg aaattcggcg atcacgctgc caagctggtg agattcagca
     5281 acgtgggcgt gggtggcgtc atcgtggctg gcaagcgcaa tttttcgcgc gcggcacagg
     5341 gcggcgatgg attcgcgatt cggctgcgac cagcgtgcgg cgagcgccag ttgctcttct
     5401 tcgtactgct gcatctgcgc atcagtgagg gagtatttgc cctgataata ttcgcgatac
     5461 ttctcgcgat tggcgaactg gcgctggccc ggcgagtggt ccatcagcga caccagcgtc
     5521 accggctcgc gctgcaccag tttttcaaac agcggcagcg tggtgtgatg cggcagttcg
     5581 cagcgcagat gcagacggtg ctcggcgcgg ttgacgccgc gtttctgcgt ctcttcgatg
     5641 gcgttgatca tcttctccag attcaccagc cgatcgccgc cgtcgcgcac gtcgccaatt
     5701 gccacggcat ccagtacggt ggtgatgccg ctcgccacca tcagcgcgtc gtggctgctc
     5761 atcgccgagt gggcaggcca gtcaactttc gggcgcgggg tgaagaattt atccagatta
     5821 tcggtatgca gctcaatcag ccccggcagc agccagccgc cttcgccgtc catcgcctcc
     5881 ggcaggcggc tctggctttc ggcaaaggcg cggatttcgc cgtcctgcac ctccagtgaa
     5941 ccgtgtacca cttcgttttc cagcaccaat ttgacgttat tgataatcat gaagaggctt
     6001 ccattgggtg caggcggtcg gcaacgtcat tacgtacggc ttcgtcgtgg aagatgccga
     6061 cgatggccgc gccacgggct ttggcttcgt gaatcagctc gactacggcg gcgctgtttt
     6121 tggcgtccag cgaggcggta ggttcgtcga gcagcagaat ggggtagtcg acgataaagc
     6181 cgcgggcgat gttgacgcgc tgctgttcgc cgccggagaa ggtcgatggc gccagatgcc
     6241 acaggcgttc cggcacgttc aggcgggtga gcagacgcgc ggctttagcg gcgcaggctt
     6301 cacgcggtac gccggtatcg agtagcggct gcatcaccac ttccagcgcc gaaatacgcg
     6361 ggatgacgcg cagaaactgg ctcacccagc caacggtggt tttgcggatc tccaccactt
     6421 tgcgcgctgg cgcggtgacc agatcgaccc attcgtcacc gtgtttgatc tggatttgcc
     6481 cttcgtcggg cagatagttg gcgtacagcg agcgtagcag ggttgatttt ccgctgccgg
     6541 aatggccgtg gagcaccacg cattcgcccg cgttgacgat gagcgaggcg cggttgagga
     6601 cgggcaggcg cacgccgttt tgctggtgca ggatgaaggt tttactgacg ttttgtacgt
     6661 taatcatttt tcgcctcgtg gttcgggtgt cggatgcgac gctgacgcgt cttatccgac
     6721 ctacggggag cgcatttgta ggccggataa ggcgtttacg ccgcatccgg caccggctca
     6781 attctgcaaa accgatgaca ccagcagctg tgtatacgga tgatgcgggt cgtcgagcac
     6841 gcggtcggtt aacccactct ccaccacttg cccctgcttc atcaccagca aacggtccgc
     6901 cagcaggcgg gcgacgccta aatcgtgggt gacaatcact accgcgaggt tcagctccac
     6961 caccagtccg cgcagcaggt cgagcaggcg ggcctgcacc gacacatcca gcccgccggt
     7021 cggttcatcc ataaacacca gcttcggatg cgtcaccagg ttgcgggcaa tctgcaaacg
     7081 ctgctgcata ccgccggaaa aggtggtcgg caggtcgtcg atacggttgg cgggaatctc
     7141 cacctcttcc agccacttct gcgcggtagc gcgaatatcg ccgtaatgtc gcgccccggt
     7201 cgccatcagc cgctcgccga tattgccgcc tgccgacacc tggcggcgca ggccgtcgag
     7261 cggatgctga tgcaccacgc cccattcggt gcgcagcagg cgacggcggt cggcctcgct
     7321 cattgcatag agggaacggt tttcatagtg aatctccccc tgctgcggcg tcagacgcgc
     7381 ggagatcgac ttcagcagcg tggtcttccc ggagccggat tccccgacaa tgcccagcac
     7441 ttcccccggc cataaatcaa aagagacatc gctaaagcct ttgcccggcg cgtaaaggtg
     7501 ggtcaggtta ttgaccgaaa gtaacggttg attcattggc ttttcgcctc gctctgttgg
     7561 cggcaataat cggtatcgga gcagacaaac atgcggtttc cggcgtcatc cagcaccact
     7621 tcatcaagat agctgtgggt cgatccgcag atggcgcatg gctcatccca ctgctgaacg
     7681 gtgaacgggt gatcgtcgaa atcgagactt tccacgcggg taaacggcgg caccgcatag
     7741 atgcgcttct cgcgccctgc gccgaacagc tgcagggcgg gcatcatgtc cattttcggg
     7801 ttatcgaatt tcgggatcgg cgacgggtcc atcacgtagc gcccgttcac cttcaccgga
     7861 taagcatagg tggtggcgat atgaccgaag cgggcgatat cttcatacag tttcacctgc
     7921 atcacgccgt actcctccag cgcgtgcata gtgcgggtct ccgtttcgcg cggctcgata
     7981 aagcgcagcg gttccgggat tggcacctgg aagataatga tctgatcttc ggtcagcggc
     8041 gtttcgggga tgcggtgacg cgtctggata agcgtcgcat cgtccgtaca ttcagtggtg
     8101 tttaccccgg tcacgcgctt aaagaagttg cgaatcgaca cggcgttggt ggtgtcatcc
     8161 gcaccctggt caatcacctt caacacgtcg ctttcgccaa tcacgctggc ggtgagctgg
     8221 ataccaccag ttccccagcc gtacggcatc ggcatctcgc gcccgccaaa cggtacctga
     8281 taaccgggga tcgccaccgc ttttaagatg gcgcggcgga tcatgcgttt ggtctgctcg
     8341 tcgaggtagg caaagttgta gccgctcaga ttagccatgg ttctgctcct gttgcagacg
     8401 tttgagtagc tccagttcgg cctggaaatc gacgtagtgc gggagtttga ggtgcgagac
     8461 aaagcctgcg gcttcgacgt tgtcggcatg cgccagcacg aactcttcat cctgcgccgg
     8521 gcctgtcgcg tgctcgccgt attccggggc ttgcaacgca cggtcaacca gcgccattgc
     8581 catcgctttg cgctcgctca tgccgaatac cagcccgtag ccgcgcgtga agtgcggcgg
     8641 ctcatccggc gggtcgataa aaccgttgac catttcacac tcggtcatca gcagttcgcc
     8701 gacgtttacc gcaaatccca gctcttccgg cacaatcgac acgtcgatgt aaccactgcg
     8761 gatctcgccc gcgaacgggt ggttgcgccc gtaaccgcgc tgggtggagt aagccagcgc
     8821 cagcaaatag ccttcgtcgc cgcgcattaa ctgctgcaaa cgggaagaac gtgaacaggg
     8881 gtaaaccggc ggcgtgcggg tgatgtcatc cggctgtgct ccgctatcct cttcaaactt
     8941 cgccagcccc tgacgcgcca gcaggctgaa aacgtgcggc gacggctgtt gttcgctgtc
     9001 ggcggtggtc agcgtcggcg cttcgccgtt tgccagcaga gtgaaatcga gcaggcgatg
     9061 ggtgtagtcg taggttgggc caagcagctg gccgccggga atatctttat aaaccgctga
     9121 aatacgccgt tcgagacgca tctccgtggt gtcgagcggc tcgcttaccg ccagtttcgt
     9181 cagcgtggtg cggtaggcgc gtagcaggaa aatcgcttcg acgttatcgc cgctggcctg
     9241 tttcagcgcc agcgccgcca gttcgcggtc ggcaatgccg ccttctgtca tcacgcgatc
     9301 caccgcgagg ttaagctgct gttcaatctg ggcgacgctc agttcgggca aatcggtatc
     9361 gccccggcgt cggctctctt gcagggcgtg ggcggcgtcg atcgcctttt cgcccccttt
     9421 cacggcaacg tacatcagca cacctccaca tgagtggttc gcgggatagc cagcaggcgc
     9481 tcgccgcagg tcaggattag gtcgatgccg agcgggaacg ggtgcgggcg ctcggtgagt
     9541 tcgtgcagaa tgcactctgg cagctgcgga gcgatcattc gttcttcggc aatgcccgcg
     9601 ccggtgagac gcaacatgcg tccgccgctc aggctggcga cctgtaaaat cagcgtcgcg
     9661 cccgcttccg gcgcaacggc ggtgtcggtg gaaagggcgt tgagctgttc gctggaaatc
     9721 gcctcatccg tcaccgcgaa ggtcgcctgt tccggctggc tgaccagcgg cgcgttggta
     9781 tgaaaacgca ggctctgatt gacgatatcg ttacttaatg gcgtggcaag ccacaccggc
     9841 gtgtcgttat cggccagcgt cagcagcacg ctggtggtgg cgatattcag cggctgccag
     9901 ccgcgtttga gctgatacag ggcgacaatc acgcccggct cgctcatggc ctttaacagg
     9961 cgacgaaaac tgtgctgggc atcctgcacg ggaagcataa aagcggtttc cagggtcatg
    10021 cgttgtctcc gcgaaccatc gtaaagaagt cgacccggct ggcgttcact tcggcctggc
    10081 gtgcggcaat gcgtgccata cggtcagcgt ccagcggggc aataagggtt tctgataagt
    10141 tttgaaagtg gcgagattgc tgcatcagcg catcaatcag cgcgcagcgt tcggcgtgct
    10201 gtttatcacg ccccagcacc cagctgtagc cgagggtgcc gtcagtcagg cgtacggcgg
    10261 cacgggtcag cgtggcgtcg ccggcaaaaa agcgttcgcc ggtaccgccc attcgcgcct
    10321 gaatctgcac caggccagtt tcggcagtgc ggatcacttc atagtcggcg gtgatgttca
    10381 gcgcgttcag gcgggctgcc agttcagccg gttggctgtg cgccagtacg gacatccagt
    10441 gctggcgggt cgcggtatct gcgtgcattc agtgctccat agtgaattca atcatgtcgg
    10501 cgcgcgtcag gctgacggag tactccgccg ggctgctttc accgtcacgg tggttaaggg
    10561 tgcgcacgca cagcagcggc gacatattcg ggatttcaag gcgctggcac tctttggcct
    10621 gggcgcggcg ggcgctgatc cgcgtctggc tgcggcgcag cgcaattccg gtttgctcgc
    10681 gcagaaaatc gtgcagcgaa ccgctgtcga aacgttgcag tgtcggccag agggtgaggt
    10741 ccgcaaagta gtggtcgatt aaacagagcg cgacgccgtt gacgcgacgc agggtgcgca
    10801 aatggataac gttctcaccc tcggtaatcc ccagcgcgtc agcaacgtga ccggacgcgg
    10861 ggcgcaatac cgacagcagt ttttcgctgg tgggatggct gccctggtcc agcaggttct
    10921 ggctaaaacg cgcctgggcg ttgagcgggt aatcgaacgg gcgcatcagc accagcacgc
    10981 cgatgccctg acggcgctgt acccagcctt tttccaccag ttggtcgatg gcgcggcgca
    11041 gggtgtggcg gtttacctca aagcgcgccg ccagttgctg ctcggcggga agatagtcgc
    11101 cgcagcgata gtgttgacga agctcctgct caagttttgc ggctatctcc tgatagcgtg
    11161 ttgggtagct ggtcggatgt gtagacaagt gcatagatat caatgcctca cttatcagat
    11221 aaagtgctta cgcaaccgtt gagagaggaa atccagcagg ctgaccgtga cgatgataag
    11281 caccatcagg gcgcaggttt gttggaactg gaaaccgcga atcgcttccc acagggtgac
    11341 gccgatcccg cctgcaccga ccatgccgac gacggtcgcc gagcggacgt tggattcgaa
    11401 gcgatagagg gagtaggaga tcagcagcgg cattacctgt ggcagcacgc cgtaaaggat
    11461 ctcttccagc ttgttggcgc cggtggcgcg aatgccttcc accgggccgg gttcaatcgc
    11521 ttccaccgct tcggaaagca gtttcgagag cacgccggtg gtgtgaataa atagcgccag
    11581 cacgccagcg aacggtccga gaccgacggc gaccacgaac agcatggcga agaccatttc
    11641 gttaatggcg cggcaggcgt ccatcaggcg gcgaacgggc tggtaaaccc accacggcac
    11701 caggttttcg gcgctcatca ggccgaaggg gatggagaga accactgcca gcgcggtgcc
    11761 ccagacggcg atttgcagcg tgacggccat ttcggtgagg taatcctgcc actggctgaa
    11821 atcgggcggg aagaagtcgg cggcgaaggt cgccatgttg ccgccgtctt tgatcagcgt
    11881 aagcggggcc atttccgcgc cctgccacga gacgaccaac acggcgagaa caatggccca
    11941 gctcagaagc gagaaccagc tgcgcttggg tggggcgatg gtgatggttt gcatgtttgg
    12001 ctccgtttaa tgttttctcc ctctccctgt gggagagggc cggggtgagg gcatcagcgt
    12061 gcgcatttcc ccctcaccct aaccctctcc ctcaagggag aggggaccga tcgagcacat
    12121 tactgcaccg ctttactcac cgaactcatc gcgcttaacg cgttgttcag gcggtccagg
    12181 tcatccagct gcgcctgaat ctcggtggtt ttcgccagct tgtcctgctc attcagtccg
    12241 ttattgcttt ttacgccctg aatctcttta aacagtgcga gctggcgaat cggcaccagt
    12301 tgcaggtcgc tggaggcgcg aaacggtgcc cagcccaggc gttccagcac cgctttctct
    12361 tccggcgttt ttccgtagtt cataaagaag tcgtagatct tgtctttggt ggtttcggag
    12421 agattcttgc gccagacgat cggatcgcct gggatcagcg gcgacttcca gatcaccttc
    12481 agttctttca gcttctctgg cgcggaggtt ttcagcttgt cgaggttttc ggtgttgttg
    12541 gtggcaacat ccacctgctt gttggcgacg gccagcgcgt tggtttcatg cccggcgttg
    12601 acggtgcgct taaagtcgct ggcggagata ttgtttttgg caaagacgta gtaaccgggg
    12661 acgaggaagc cagaggtgga gttaggatcg ccattgccga aggtgagatc tttccgcttc
    12721 gccagcagat cgttcaggtt gttgatcgga ctatctttgt tgacgatcaa cacgctccag
    12781 taacctggcg atccatccgc cgcgaccgtc tgggcgaaga cctggccgtt ggcgcgatcc
    12841 accgcttcca ttgccgacag gttgccgtac caggcgatat ccactttatt gaagcgcatt
    12901 ccctggataa tgcctgcgta gtctggggca aagaaggcgt tcaccttcac gcccagcttc
    12961 ttctccatat cctgtaagaa tggcgtccat tgcggtttca ggttttgctg tgattccgtt
    13021 gaaataatgc cgaaattcag cgccttttcc tgctcttcgg cgtgtgccgg gcttaacagg
    13081 gtgctgaggc tgaacatgct ggtgaaggcc agcgaggcaa ttatcttagc gttcattcgt
    13141 tttcctcaat gatggggatg tcaggcagct ttcgcgttct cttcgatgcg attaatgctg
    13201 cggtagagat ggtcaaaacg ttcgttatca aactgttggc tgctgccgtc gtagaaaacg
    13261 tgcccctggc gcagggcgac gatgcgttcg cagtagcgca gggcgtaatc cacctgatgc
    13321 agcgtgacga ccacggtgat gccgtcgttc tgattgatgt cgcgcagggt gtccatcacg
    13381 atgcgggcgg attccgggtc cagcgaggcg atgggttcat cggccagaat caccttcgcc
    13441 tgctgcatca gcgcgcgggc aatcgccaca cgctgctgct gtccgccgga gagggtggaa
    13501 acgcgttgat gggcaaaatg caccatgcca acgcgggtca gcgcctgtaa cgcgcgttgt
    13561 ttctgctcgc gggtaaacca gctaaaacag gtgcgccaga acggcgtgct gccgagcgcg
    13621 ccaatcagca cgttctccag tacgctcagg cggttcacca ggttgaattg ttggaagatg
    13681 tagccggtgt tggcgcggct tttgcggata tcgcgcgcca gacggccttc gcgctggact
    13741 gtgcggccca gcagctcgat atggctgccg gcggatttat cgccggtaat caaaccgctt
    13801 aagtgacgta aaagggtgga tttgccggaa cccgacggcc caagcagagc caccatttca
    13861 ccgtgatgaa tgttcagagc aaccgcatgc agcgcctgat gctgattgaa ggttttggcg
    13921 agcttctcga cacggataat cgtttgcatg atgcagcctc cctaaaaaag tggctccatc
    13981 gtggcggatt attgtgacga tttggttaat taaaagtgac taacagatga agagttaacg
    14041 ggaattcgat gacagtgcgg gagggcgggc ctcccggcgg gttattgcgt tggttgttgt
    14101 ttgacgacat taatcatcca cggtacgcca aatttatcgg tgactttgcc aaagccatgc
    14161 gcctagaaag tttcctgcca ggccatttcg atttttccgt tagcggcaag attgtcaaac
    14221 cagcgttttc cttcttcgac ctgttgcgaa tcgagcacca gcgtaaagcc ggagtagctg
    14281 gcttttcctg acggcatggc atcgctcatc atgatgtcgc ttccggcaat gcgcacattg
    14341 gcgtgggcga tggcggtatc gggaaattgc attccggaag ggcagttctc ggcgctgtcc
    14401 tgcgctgatt ttggcatttc gccgaagctg attttataga gcagttcagc gcccaacgta
    14461 cgttgataat aagcaattgc gtcggcacag ttaccggcaa aagagaggta gggacttaac
    14521 ggcatgatgg tgacctcagt taagagaagc caattaagtg tagttcgaaa tttatacaga
    14581 tgagaggctg cctgata
//
LOCUS       DAZPAC010000099        14404 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZPAC010000099 DAZPAC010000000
VERSION     DAZPAC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.099"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="ACHUPM_002485"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="ACHUPM_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR0870232.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="ACHUPM_002486"
     CDS             1828..3147
                     /locus_tag="ACHUPM_002486"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR0870233.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3519..4901
                     /gene="dosC"
                     /locus_tag="ACHUPM_002487"
     CDS             3519..4901
                     /gene="dosC"
                     /locus_tag="ACHUPM_002487"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR0870234.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEKFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            4926..7325
                     /gene="dosP"
                     /locus_tag="ACHUPM_002488"
                     /gene_synonym="pdeO"
     CDS             4926..7325
                     /gene="dosP"
                     /locus_tag="ACHUPM_002488"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFR0870235.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7584..8165
                     /gene="ddpX"
                     /locus_tag="ACHUPM_002489"
     CDS             7584..8165
                     /gene="ddpX"
                     /locus_tag="ACHUPM_002489"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFR0870236.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8179..9729
                     /locus_tag="ACHUPM_002490"
     CDS             8179..9729
                     /locus_tag="ACHUPM_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0870237.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9731..10753
                     /locus_tag="ACHUPM_002491"
     CDS             9731..10753
                     /locus_tag="ACHUPM_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0870238.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10750..11646
                     /gene="ddpC"
                     /locus_tag="ACHUPM_002492"
     CDS             10750..11646
                     /gene="ddpC"
                     /locus_tag="ACHUPM_002492"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR0870239.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11643..12629
                     /locus_tag="ACHUPM_002493"
     CDS             11643..12629
                     /locus_tag="ACHUPM_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0870240.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            12658..13548
                     /locus_tag="ACHUPM_002494"
     CDS             12658..13548
                     /locus_tag="ACHUPM_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR0870241.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="ACHUPM_002495"
     CDS             complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="ACHUPM_002495"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR0870242.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3578 a         3558 c         3628 g         3640 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gcttttttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggca cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcaaagacc ttcttccctg acttctctaa agtgggcacc ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcatc cgcaacccac gtcaatgaaa
      841 tgagcaaccc agggcgcgac tatccgttgg ctatgttact gctgatggta gcggcaatct
      901 gcttaagctc tgttggtggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcacagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcta ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ctctaccgat atgtatgttg
     1501 aattactggt cgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgctcgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcatatatg gatatctgct cccgaaacga gaaattagcg
     1861 attagaagac cagcgatact ggttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggtgt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggtataaaca cggtcttttt ccaggttaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccat ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgca gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgag tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgca aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaagt atcgcacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ctaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtccta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtca atctcgcacg ttgcgttttg
     3481 attccgtctg atttcccagc cttataaggg tgtgaattat ggagatgtat tttaaaagaa
     3541 tgaaagatga gtggaccgga cttgtcgaac aggcagatcc actcattcgt gctaaagccg
     3601 cggaaattgc cgttgcgcat gctcattatc tgagtatcga gttttatcga attgtccgca
     3661 tcgacccgca tgccgaaaaa ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg
     3721 cgatggaacg ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa
     3781 tacaaatcca gcataccgtc gcggaagtgc atgcccgcat aggaattccg gtagaaattg
     3841 tcgagatggg gtttcgggtg ctgaaaaaaa tcctctatcc ggtaatcttc tcttcggatt
     3901 attccgccgc agaaaaactt caggtctatc atttctcgat taacagtatt gatatcgcga
     3961 tggaagtgat gacccgcgcg tttaccttta gtgacagtag tgcctcaaag gaagatgaaa
     4021 attatcgtat cttctcgtta ctggaaaacg ccgaagaaga aaaagaacgg caaatagcct
     4081 caatactttc atgggaaata gatattatct ataaaatcct gctggattct gatttaggca
     4141 gtagtttgcc attaagccag gctgattttg gcctgtggtt taaccataaa ggtcgacatt
     4201 attttagtgg cattgccgaa gtaggccata tctcccgtct cattcaggat ttcgacggta
     4261 ttttcaatca aaccatgcgt aacaccagga atttgaataa cagaagtctg cgggtgaaat
     4321 ttttattaca gataagaaat accgtatcgc agattattac cttgctgcgt gaattgtttg
     4381 aagaagtatc gcgccacgaa gtcggtatgg atgtactaac gaaattactt aaccgccgtt
     4441 tcctgccgac tatcttcaaa cgcgaaattg cccatgccaa ccggaccggt acaccgctgt
     4501 cagtgctgat tattgacgtt gataaattca aagagatcaa cgatacgtgg ggccataaca
     4561 ctggcgatga aattctgcgt aaagtctctc aggcctttta tgacaacgtc cacagtagtg
     4621 attatgtttt tcgctacggg ggcgatgaat ttatcattgt tttgactgaa gcttctgaaa
     4681 acgaaacgtt acgtaccgca gaacgtattc gcagtcgggt ggagaaaacc aaactgaaag
     4741 ccgcaaacgg cgaggatatt gccctctcac tttccatcgg tgccgccatg tttaatggtc
     4801 atcctgacta tgagcgcctc attcaaatag ccgatgaagc tctgtatatc gccaaaagac
     4861 gaggtagaaa ccgtgttgaa ctctggaaag ccagtcttta gatgcgccag gatgcagagg
     4921 taatcatgaa gctaaccgat gcggaaaatg ccgccgatgg catttttttc cccgcccttg
     4981 aacaaaatat gatgggcgcg gtgttaatta acgaaaatga tgaagtgatg tttttcaacc
     5041 ccgccgcaga gaagctctgg gggtacaaac gtgaagaagt cattggcaat aacattgata
     5101 tgctgattcc acgggatttg cgtcctgcgc atcctgaata cattcgtcat aaccgtgaag
     5161 gtggtaaagc gcgcgtcgag gggatgagtc gggagctgca gctggagaaa aaagacggca
     5221 gtaaaatctg gacccgtttt gcgctatcga aagtgagcgc cgaggggaaa gtttattacc
     5281 tggcgctggt acgggatgcc agcgtagaaa tggcgcaaaa agaacagacc cgacaattga
     5341 ttattgccgt tgaccatctc gaccgaccgg tgattgtcct cgatccggaa cgccatattg
     5401 tgcagtgcaa tcgcgcattt accgaaatgt ttggttactg cattagcgaa gccagcggta
     5461 tgcagcccga tacactcctg aacattcctg aattccctgc cgataaccgc attcgtttac
     5521 aacagttgct atggaaaacc gcccgcgatc aggacgaatt tctgctgttg acacgcaccg
     5581 gtgaaaaaat ctggattaaa gcctctatca gcccggttta tgacgtgctc gcgcatctgc
     5641 agaacctggt aatgaccttc tcggatatca ccgaagaacg gcagatccgc cagcttgaag
     5701 gcaatattct cgccgccatg tgcagcagcc cgccatttca tgaaatgggg gaaatcattt
     5761 gtcgtaacat cgaatctgtt ctcaacgaat cgcatgtttc gctgttcgca ctgcgcaacg
     5821 ggatgccgat acactgggcg tcatcttcgc acggtgcaga agttcaaaat gcgcaaagct
     5881 ggtcagcgac cattcgtcag cgtgatggtg cgcctgcggg gatcctgcaa attaaaacct
     5941 cgtcaggagc agaaaccagc gcctttatcg aacgcgtggc agatatcagc cagcatatgg
     6001 ccgcgctggc gctggaacag gaaaaaagcc gtcagcatat tgaacaactc atccaatttg
     6061 atccgatgac cggtctgcca aatcgcaata acctgcacaa ttacctcgat gaccttgtcg
     6121 acaaagccgt ctctccggtg gtgtatctca tcggtgttga ccatattcag gatgtgattg
     6181 atagccttgg ctatgcgtgg gccgatcagg cattgctgga agtggtcaat cgctttcgtg
     6241 aaaaactcaa accggatcag tatctctgtc gtatcgaagg tacgcagttt gtcctcgtga
     6301 gcctggaaaa cgacgtcagt aacattaccc aaatcgccga tgagctacgg aatgtggtca
     6361 gcaagccgat aatgattgac gataaaccat tcccgctaac cttgagtatt ggcatcagct
     6421 acgacgtggg taaaaaccgc gattacctgc tctccactgc tcacaatgca atggattata
     6481 ttcgcaagaa tggcggtaac ggctggcagt tcttcagccc ggcgatgaac gaaatggtaa
     6541 aagagcgttt ggttttaggc gcagcgctga aagaagcgat tagcaataac caactgaaac
     6601 tggtttacca gccgcaaatc ttcgcagaaa cgggtgaact gtacggcatc gaagcccttg
     6661 ctcgctggca cgatccccag catggtcatg tacccccttc acggtttatt cctctcgcag
     6721 aagagattgg tgaaatcgaa aatattgggc gctgggtcat cgcggaagct tgccgtcagt
     6781 tagcagaatg gcgtagccag aatattcata tcccggcgtt atccgtgaac ttgtcggcgc
     6841 tgcactttcg cagtaatcag ctgcctaatc aggtgtctga tgcaatgcaa gcctggggta
     6901 ttgacggcca ccagctgacg gtagaaatca cggaaagcat gatgatggaa cacgataccg
     6961 aaatctttaa gcgcattcag atcctgcgcg atatgggcgt gggcttatcg gtagatgatt
     7021 ttggcacggg cttttccgga ttatcccgct tagtcagtct tccggtaacg gaaatcaaaa
     7081 ttgacaaaag ttttgtcgat cgttgtctga cggaaaaacg catccttgcc ttacttgaag
     7141 ccattaccag cattgggcaa agcctcaatt taaccgtcgt ggcggaaggc gtcgaaacca
     7201 aagagcaatt tgagatgcta cgcaagatcc actgtcgcgt tattcaggga tatttctttt
     7261 cccgccccct acccgccgag gaaattccag gctggatgag cagcgtgtta ccgctgaaaa
     7321 tctgacaaat tcctctcgcc cgcactcgcg ggttttcatt taacgtgacg ctgtcactta
     7381 aatactgtga tttcagctac gattccggga gattccttcc ttaacttccc ctcttgcacc
     7441 aaaactgcac tacgctgcac aaatgttgat caaaagttaa aaaatataac tttaagccag
     7501 cattctggcg cttatcccgg catggcatga gatctgcata agcggaaagc gcagcaattt
     7561 ttgtcttaat acaggaaccg tttatgtcgg ataccaccga actggttgat ttagccgtga
     7621 tcttccctga tctggagatc gaattgaaat acgcctgcgc tgataacatc acaggtaaag
     7681 ctatttacca gcaagcgcgt tgtctgttac acaaggatgc gattaccgcg ctggcgaaaa
     7741 gtatcagtat cgcccagctg tcaggcttac aactggtgat ttacgatgcg tatcgcccac
     7801 aacaagcaca ggcgatgttg tggcaagcct gcccagaccc gcaatatgtt gttgatgtga
     7861 cggtcggttc taatcacagc cgtggcacgg cgatcgacct gacgcttcgt gatgagcacg
     7921 ggaacatcct ggatatgggc gcagggttcg atgaaatgca cgagcgctcc catgcctatc
     7981 acccttccgt cccgcccgcc gctcagcgca atcgcctgtt gctgaatgcg ataatgactg
     8041 gtggcggttt tgtcggtatc tccagcgaat ggtggcactt cgaattaccc caggcagcga
     8101 gttaccctct gctcgctgat caattctctt gttttatatc ccctggcaca cagcacgtca
     8161 gttaatcagg aaactgtcat gaagagatcg atatcgtttc gtcccacatt gctcgcgctc
     8221 gtccttgcca caactttccc ggttgcgcac gccgccgtac cgaaagatat gctggtgatt
     8281 ggtaaggccg ccgatccaca aaccctcgac ccggcggtaa caatagataa taacgactgg
     8341 acagtgacct acccgtctta tcagcggctg gttcagtaca aaacggacgg tgataaaggc
     8401 tcaaccgacg ttgaaggcga tctggcaagt agctggaaag cgtctgacga tcaaaaagag
     8461 tggacgttca ccctgaagga caatgctaaa tttgccgatg gcacacctgt cactgccgaa
     8521 gcagtaaaac tctcttttga gcggttacta aaaatcggcc aggggccagc agaagcattt
     8581 cccaaagatt taaagattga tactcccgac gaacatacgg tgaagtttac ccttagccaa
     8641 ccattcgcac cgttcctcta cacgctagcg aatgacggtg catccattat caatccggcg
     8701 gtcttaaagg aacatgcagc ggatgatgct cgcggcttcc tcgcgcaaaa taccgccggt
     8761 tccggaccat ttatgctgaa aagcgggcaa aaaggtcagc aattagttct ggtgccaaat
     8821 ccgcattacc ccggcaataa accgaacttt aagcgagtat cggtaaaaat tatcggtgaa
     8881 agtgcctccc gtcgcctgca gctctcccgt ggcgacattg acattgccga tgcgctgccg
     8941 gtggatcaac tcaacgccct gaagcaggaa aataaagtca atgtggcaga gtatccgtca
     9001 ctgcgcgtta cctatctgta tctgaataac agcaaagcgc ctcttaatca ggcggatctg
     9061 cgtcgggcca tttcctggtc taccgattat cagggcatgg ttaacggcat tctgagtggt
     9121 aacggaaaac agatgcgcgg cccgattccg gaaggcatgt ggggctacga tgcgacggca
     9181 atgcaataca accatgacga aacgaaagcc aaagccgaat gggataaagt gacgagcaaa
     9241 cccaccagcc tgacgtttct ctactccgat aacgatccga actgggagcc tattgctctg
     9301 gcgacacaat ccagtctcaa caagctgggc atcaatgtga agctggaaaa gctggcgaac
     9361 gccaccatgc gcgacagagt gggtaaaggt gattacgaca ttgcgattgg caactggagt
     9421 ccggattttg ccgacccgta tatgtttatg aattactggt ttgagtcaga caaaaaaggt
     9481 ctgccgggta accgctcgtt ctatgaaaac agtgaggtcg ataagttact gcgcaatgcg
     9541 cttgcgacca ccgaccagac gcagcgtacc cgggactacc agcaggcaca gaaaatcgtc
     9601 attgatgacg ctgcttatgt gtacctgttc cagaaaaact accaactggc gatgaacaaa
     9661 gaggtgaaag gctttgtgtt caatcccatg ctggaacagg tcttcaatat caataccatg
     9721 agtaaataac gtgaccttct ggagtatttt acgccaacgc tgctgggggc tggtgctcgt
     9781 ggtggcgggc gtctgcgtga ttacgtttat tatctcgcat ctgatccctg gcgatccggc
     9841 gcggttactg gcgggtgacc gcgccagcga tgctatcgtg gaaaatattc gccagcaact
     9901 gggactggac cagccactgt atgtacagtt ttaccgctac ttcagcgacc tgtttcatgg
     9961 tgacctggga acatccattc gtaccgggcg tccggtgctg gaagagttgc gtatattttt
    10021 cccggcgacg ctggaactgg ctttttgtgc cctgctgctg gcactcctga ttggcatccc
    10081 gctgggcata ctctctgcag tctggcgaaa tcgctggctg gatcatctgg tgcgaataat
    10141 ggccattacc ggaatctcca cacctgcgtt ctggcttgga ctgggcgtca ttgtgctgtt
    10201 ttatggtcat ctgcaaattc ttcccggcgg cggaaggctt gatgactggc tggatccacc
    10261 aacgcacgtt accggctttt atctgctcga tgcgctgctt gaaggcaacg gtgaagtctt
    10321 cttcaatgcg ttgcaacatc tcatcttacc ggcattaacg ctggcgttcg ttcacctggg
    10381 aattgtcgct cgccagatcc gctcagcgat gctggaacaa ttgagtgaag actacattcg
    10441 taccgcccgg gccagcggct tgcccggctg gtatatcgtt ttatgttatg cgctacccaa
    10501 tgcgttgatc ccatcgatta ccgtattggg tctggcgctg ggcgatttgt tgtatggcgc
    10561 agtgctcacc gaaaccgttt ttgcctggcc cggaatgggt gcatgggtag taacatcaat
    10621 acaggcgctc gacttcccgg cagtgatggg ctttgccgtc gtggtttcat ttgcttatgt
    10681 gctggtcaac ctggtggtgg atttgctcta tttgtggatt gatccgcgta tcggacgtgg
    10741 aggtggtgaa tgatgctaag cgaggaaacg tccgccgtac gcccacaaaa acaaacgcga
    10801 tttaacggtg caaaactggt atggatgctg aaaggcagtc cgctcaccgt gaccggcgca
    10861 gtcatcattg tattaatgct attgatgatg attttttcac cgtggcttgc gacgcatgat
    10921 cccaacgcca ttgatttaac cgcccgcctt ttgccgcctt ctgcggcgca ctggtttggc
    10981 accgatgaag tgggacgcga tctgttcagc cgcgtactgg tcggcagtca gcaatcaatt
    11041 ctcgccggat tagtcgtggt tgccattgcg ggtatgattg gttcgctact cggatgtcta
    11101 tccggtgtgc ttggcggacg cgcagacgcc atcatcatgc gcatcatgga cattatgctg
    11161 tcgattcctt cgttggtact gacaatggca ctggctgccg ctctcgggcc gagtttattt
    11221 aacgccatgc tggcgattgc tattgtgcga attccctttt atgtgcgcct ggcgcggggg
    11281 caagcattag ttgtacgcca gtatacctat gttcaggcgg cgaaaacctt tggtgcgtcg
    11341 cgttggcatc tgatcagctg gcatatttta cgtaactccc tgccgccgct gatcgtgcag
    11401 gcatcgctgg atatcggtag cgcgatttta atggccgcca cgttgggatt tattggcctg
    11461 ggtgctcaac aaccgagtgc tgaatggggc gcgatggtag ctaatggtcg caactatgta
    11521 ctcgatcagt ggtggtattg cgcatttccg ggggcagcga ttttgcttac cgccgtcggg
    11581 tttaatctct ttggtgatgg tattcgcgat ctgcttgacc cgaaagcagg aggaaagcag
    11641 tcatgaccca acccgttctg gacattcaac agctgcattt gagtttcccc ggttttaacg
    11701 gttatgttca cgtgctcaac aatgtgtcct tgcagattaa ccgcggtgaa attgtcggtc
    11761 tggtgggaga atccggctca ggtaaatcag tcaccgcaat gctgattatg cgtctgctac
    11821 cgacgggcag ttattgcgta catcgggggc agatttcact gctgggagac gatgttctta
    11881 acgctcggga gaaacagctt cgtcagtggc gcggcgcacg agtggcgatg atctttcagg
    11941 aaccgatgac cgccctcaat ccgacacgtc gaataggtct tcagatgatg gacgtgatcc
    12001 gccatcatca accaataagt cgtcgggaag ccagagctaa agcgattacc ctgctggaag
    12061 agatgcaaat cccggatgcc gtggaagtta tgtcgcgcta tccgtttgag ctttcaggtg
    12121 gtatgcgcca gcgggtaatg attgcgctgg cattctcctg cgagccgcaa ttgattattg
    12181 ccgacgaacc gactacggcg ctggacgtca cggtacagtt gcaggtactg cgtttgctta
    12241 aacataaagc tcgcgccagt ggaactgcgg tactgttcat cagccatgat atggccgtgg
    12301 tgtcgcaact gtgcgatagc gtttacgtga tgtatgccgg aagcgtaatt gaaagcggcg
    12361 tgacggcaga cgttatccat catccccggc atccgtatac cattggtttg ctgcaatgcg
    12421 caccagaaca tggagtacca cgccagccat tacccgccat tccagggacg gtaccaaacc
    12481 tcacccattt gcctgacggc tgcgcttttc gcgatcgttg ctatgcggca ggtgcacagt
    12541 gtgaaaacgt cccggcgctg acagcgtgtg gtgacaacaa ccagcgctgc gcctgttggt
    12601 atccgcagca ggaggtcata agtatctgac acgttattaa cgttacgcga cgtccatatc
    12661 aatttcccgg cccgtaaaag ctggcttggt aaaactacgg aacatgttca tgcgattaat
    12721 ggtattgatt tacagatccg ccgtggtgaa accttaggga tcgtcggtga gtcaggctgc
    12781 ggcaaaagca ccctcgcaca gcttttaatg ggtatgctgc aaccgagcca cgggcaatac
    12841 atccgttcag gatcacaacg cattatgcag atggtgtttc aggacccgct ctcttcgctt
    12901 aatccgcgct tatcggtgtg gcgcatcatc acagaaccgc tctggatagc taagcgtagt
    12961 agtgaacaac agcggcgagc gttggcaaag gagttggctg tgcaggtggg tattcgtccg
    13021 gagtatctcg accgcctgcc tcatgcgttc tccggcgggc agcggcaacg catcgccatt
    13081 gccagagcac tctcttcgca gcctgacgtg attgtgcttg atgagccaac ctcggcgctg
    13141 gatatctccg tgcaggcgca gatcctcaat ttactggtaa cgctacagga aaatcacggg
    13201 ctgacttatg tgctgatttc acacaatgtc tcggtgatac gtcatatgag cgatcgggtg
    13261 gcggtgatgt atctcgggca gattgtagaa ctgggcgacg cgcagcaggt gctgacggca
    13321 cctgcacatc catacacccg attattgctg gattccctcc ccgccattga taaaccgctg
    13381 gaggaagaat gggcattatg taaaacggat ctgccgggaa accgcacgtt gccacaaggc
    13441 tgttttttct acgaacgttg ccctctggca acccacggat gtgaagtccg gcaatcatta
    13501 gcaataaggg aggacggacg tgagctccgg tgctggcggg cgctgtagag atgctaacaa
    13561 cgcatcaggc attcacgcgc ctgacgcgtc atccggcaat gctttacgat ttcaactggt
    13621 aatccagcgt aatttccgct ttcagtacct gagaaaccgg gcatcctgct tttgctttct
    13681 ggatgatgcc gtcaaaggta gaggcatcaa tacccggcac cgcaacttca ctcttcagtg
    13741 cgattttcgt aatcgcaaaa tcggcatcca ctttatccag cgacacatcg gcggtggtat
    13801 caatcgatgt tggcgtgaat cccgcttccc ccagcattaa tgaaagcgcc attgagaaac
    13861 atgcggcatg cgctgcgcca atcagttctt cagggttggt tcctttttcg ccttcaaaac
    13921 gcgtgttaaa tccatacggc tgttggttca gcacgccact ctgggtggat actgttccct
    13981 tcccgcgttt gatatcgcct tcccagtgtg cctgaccttt cttatggatt gtcattgttg
    14041 ctctcctgtg ggcttaaaaa ggaaattata gactacgtga gaaacagcac gaggataagc
    14101 aggatattcc gaataaaata cggcgaaaat attacttctg cacttaaatt aaattcccac
    14161 aatagataac cgggaatata tatctcaaga gatattgcat caatatcaat caactcacca
    14221 caataataat acatatcaat atcatggagt taaaatataa aaatcatcaa ccaggactaa
    14281 tcttaacaac gaagcggcaa atatttgcca tgctgaatgt gctttataaa tctgcgatcc
    14341 gtagcagaca ccataaatac acagacacgg agaatcacta tgtttactta ttatcaggca
    14401 gaaa
//
LOCUS       DAZPAC010000100        14306 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZPAC010000100 DAZPAC010000000
VERSION     DAZPAC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.100"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            19..1245
                     /gene="rtcB"
                     /locus_tag="ACHUPM_002496"
     CDS             19..1245
                     /gene="rtcB"
                     /locus_tag="ACHUPM_002496"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR0870243.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1249..2265
                     /gene="rtcA"
                     /locus_tag="ACHUPM_002497"
     CDS             1249..2265
                     /gene="rtcA"
                     /locus_tag="ACHUPM_002497"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR0870244.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2308..5013)
                     /gene="malT"
                     /locus_tag="ACHUPM_002498"
     CDS             complement(2308..5013)
                     /gene="malT"
                     /locus_tag="ACHUPM_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFR0870245.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5624..8017
                     /gene="malP"
                     /locus_tag="ACHUPM_002499"
     CDS             5624..8017
                     /gene="malP"
                     /locus_tag="ACHUPM_002499"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR0870246.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8027..10111
                     /gene="malQ"
                     /locus_tag="ACHUPM_002500"
     CDS             8027..10111
                     /gene="malQ"
                     /locus_tag="ACHUPM_002500"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR0870247.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="ACHUPM_002501"
     CDS             complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="ACHUPM_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0870248.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="ACHUPM_002502"
     CDS             complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="ACHUPM_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR0870249.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="ACHUPM_002503"
     CDS             complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="ACHUPM_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR0870250.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13188..13958
                     /gene="bioH"
                     /locus_tag="ACHUPM_002504"
     CDS             13188..13958
                     /gene="bioH"
                     /locus_tag="ACHUPM_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR0870251.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13987..>14306)
                     /locus_tag="ACHUPM_002505"
     CDS             complement(13987..>14306)
                     /locus_tag="ACHUPM_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR0870252.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3442 a         3568 c         3996 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgctggga acgggtaacc actttattga aatctgcctt
      541 gatgagtcgg agcaggtgtg gattatgctg cactccggtt cacgcggaat tggtaacgcc
      601 atcgggactt actttatcga tctggcgcaa aaagagatgc aggaccaact tgagacgttg
      661 ccgtcgcgtg atctggcgta ctttatggaa ggtacggaat actttgatga ttacctaaga
      721 gccgtggcct gggcgcagct tttcgccagc cttaaccgcg atgcgatgat ggaaaacgtg
      781 gtaacggcat tgcagagcat tacgcagaaa acggtcagac agccacaaac gctggcgatg
      841 gaagagatca actgtcacca caactatgtg caaaaagaac agcactttgg tgaagagatc
      901 tacgtgacgc gtaaaggcgc ggtgtttgcg cgtgctggtc aatatggaat tattccaggg
      961 tcgatgggag caaaaagctt tatcgtccgt gggctgggaa atgaagaatc tttctgttcg
     1021 tgcagccacg gtgccgggcg ggtaatgagc cgtactaaag cgaaaaaact gttcagcgtg
     1081 gaagatcaaa ttcgtgccac cgcgcatgtg gaatgccgta aagatgccga agtgatcgac
     1141 gaaatcccga tggcgtataa agatattgat gcggtgatgg cggcacaaag cgatctggtg
     1201 gaagttatct ataccctgcg tcaggtggtg tgcgtaaaag gataaatgat gaaaaggatg
     1261 attgcgctgg atggcgcaca gggcgaaggt ggcgggcaga tcctgcgctc ggcgctgagc
     1321 ctgtcgatga taaccggcca gccatttacc atcaccagca ttcgtgccgg gcgggcgaaa
     1381 ccggggctgt tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca
     1441 acggtggaag gcgcggagct ggggtcgcag cgtctggtct tccggcttgg caccgtgcgc
     1501 ggcggcgagt accgctttgc tatcggtagt gccggaagct gtacgctggt gctgcaaacg
     1561 gtgctgcccg cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc
     1621 gataacccgt cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg
     1681 aaaataggaa ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc
     1741 ggcgtggtgg caacggaagt ctcgcccgtg gcatcgttta acaccttgca acttggcgag
     1801 cgcgggaaca ttgtgcagat gcgtggagaa gttctattag ctggtgtgcc gcgccatgtt
     1861 gctgagcgtg aaatcgctac actggcgggg agtttttccc tgcatgaaca gaatattcat
     1921 aacctgccgc gcgaccaggg gccgggtaat accgtctcgc tggaagtcga aagtgaaaat
     1981 atcactgaac gctttttcgt cgtcggtgaa aagcgcgtca gtgccgaggt ggtagccgcg
     2041 cagttagtga aagaggtgaa acgctacctg gcaagcccgg cggtagtggg ggaatatctc
     2101 gccgaccagc tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat
     2161 ccttcatgcc atctgctgac caatatcgcc gtggtggagc gtttcttgcc ggtgcggttt
     2221 ggtttgatag aaacagatgg cgtaacgcgg gtgagtattg aatgatgtaa agatgtaaac
     2281 cggatccaat gcattatccg gctaaactca caccccatat cccatcatct tcagcagttt
     2341 ctgcgcgtgt tgtaccgcgg cctgacggtg ggcgacgccg agtttttggt acagattgcg
     2401 gatatgcgtc ttgatggtgg ttgccgccac ttccagctcg cccgcaattt gctcattgct
     2461 gtaaccggag tagatcagcc ccaggacctg ccattcacgt tgggtcaacg ggctggtgtg
     2521 gatcagttct ggcacttccg gatgattcag cagacgttca acgaaattct catcgaaatg
     2581 ggcgaattta tgccgatgat gttgattgat ttctcgcaga atacgctgcg cgcgatgctg
     2641 ttccagttcc ggcagcgtat tgagttgaat cagctggcgc agttgttgcg ccatcgcttc
     2701 gccttcgatg acaaaatgac tgataaatcc ggtgcgattc gccagtttta atgcgtccag
     2761 caacacgcgc tgggcgtcac ttttacgtcc ggcctgccag tacagttgat taagcagcaa
     2821 caggttacgg ttgagatcgc tcatcaaccg cagactgcgg gcattttcat tgagttcttc
     2881 gagaacaatt tccgcaggtt caaactcgcc cagcaagatt tgtgcgcggg caatgttgcg
     2941 ccattgacct tgcaggaagt ggttgttcgc aaactccggt ttagccgtat gacgcaacca
     3001 gttggcagcg gcggctttat cgccggtcat ttgccagtaa atcacccgga ctttgttggc
     3061 gttagagatc cagtcgctgt gatatttgcc attccccagc aggttttcca gacggttcag
     3121 ctggctacgg gcgttatcta aatcaccacg ggccagcgag cattgaatca acattgccag
     3181 gcactgaagc tgttgctgtg gctgataaga cgacaagact tcaatcccgc tacgcgccga
     3241 cgcttcggct tcatccagcc gtgcccaggc ccataacagt tgtgctcgga tgcgcaccag
     3301 aaactcatgc atcggcagct gttccagatg ctgctcgttg atcagctgga acgttttttc
     3361 ctgtgtttcc cacgcggttt gcaggaaccc ttgggcaaac agaatttcac tttgctggat
     3421 taaactccat aaggcgtagt gccagacgtc gtgctggcgt gccatctgtt cggtttgctg
     3481 cattagcgcc agtgagcggg tcaattcgcc tttgcagtgc agcacttcac ccagcaccga
     3541 ggttgctaca atgcggctat agaaccagcc cggtggcagc tcttccagtg ccagttttgc
     3601 cagccgttcc gcttcatccg gattaccatc gttaatcgcc acctgggcgc gcagagcgtt
     3661 aaattctgcg tgcatggtgc cttctctgat gtccttgatt tcatgttcag cacgggctag
     3721 cagggtgtta acttcgccat agcgatgttg gctttgcatt agccacgcct gcaataacac
     3781 taactgcgga ttttccagca aactgtccca cggcagggcc ttaagcgact cttccagcag
     3841 cgacagttcg ctatggttga acaaactcca ggcgtgatta agcagaatat cgcgcagcat
     3901 cagcgcatcg cctgccgcca gcgcatgatg aatcgcttcg ctgggaaatc cctgggccat
     3961 ccagctttct gcggcggcac ggtggatttc cggcagctcc gccgccagtt cccactggca
     4021 gcgctggcgc aggaagttgc caaacagcgg gtgatagcag aaccactcgc cggtatcatc
     4081 catccgctgt aaaaacagcc cctgacgctc aatctcttcg agacgcattt gcccgttttc
     4141 ttcgccagtc acacgggtga tgagggcatc gttcattgag cgcaaaatgg cgcttttcaa
     4201 cagaaaatgg cgcgttgcga gatcgacgtt atccaaaacc tcatcgacca gataatccga
     4261 aagatggctg gcattgattc ccgccaggcg gcgtgccgac ttatgggctg agtgggtatt
     4321 ctgccgggcg gagagggcga ttagctgtag tgccgtcgcc caaccggaaa cgtcatcgca
     4381 aatccaactg ctttctgcgg cttcaatcgg cgatgacaga cggcaatcaa aaaactgctt
     4441 cgcttcctga tgggtaaatg ccagttgctg actgccaatt tccagcagtt ggtcacgaac
     4501 acgcagattg gcaatgccca gttgcggaag gttgcgtgac aacaccacca gggtgagatt
     4561 ttctggttga tggcgaataa agaagcgcat tgactcgtgg atcactggat tagtgatcag
     4621 atgatagtca tcgatgacca gataaagtgg gctatgccat tccgccagct caatgaaaag
     4681 ctgggcgaag agtgacgtca ggctggcata ttgccgtttt tgcgccatcg tctcacatat
     4741 cgcacagtga ccgttggttg cctgctgcac ggcggcaatg agatagctgg cgaaacgctc
     4801 ttgctggtta tcaccttcat ccagcgagta ccagccgata tcgtttttgc ctgccgccca
     4861 ctgggaaatg agggtggtct ttccgtagcc cgcaggactc gtgatcagcg ccagccggaa
     4921 gttgttcgcg ccggaaagtt tagccagcag gcgctcacga accacggtat ggtcgagtcg
     4981 aaccggacga cttagttttg acggaatcag catagttaat cacttcactg tggaaaatga
     5041 ggaaatatta tttttttgcg cttcgtaatt aatggttata aggtcggcca gaaacctttc
     5101 taatgcaagc gatgacgttt ttttatgtgt ctgaatttgc actgtgtcac aattccaaat
     5161 ctttattaac aactcaccta aaacgacgct gatccagcgt gaatactggt ttcccttatg
     5221 ttcatcagat tcatttaagc aagggtttct tcttcattcc tgatgaaagt gccatctaaa
     5281 aagatgatct taataaatct attaagaatg agatggagca cattggatat tttacttgtg
     5341 aaactgtttc actcctttac ttaatttata gagttacctt ccgctttttg aaaatacgca
     5401 acggccattt tttgcactta gatacagatt ttctgcgctg tattgcattg atttgctgct
     5461 aatcctgtgg tttgcactag ctttaagtgg ttgagatcac atttccttgc tcatccccgc
     5521 aactcctccc tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact
     5581 agcgataacg ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt
     5641 taacgataag caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc
     5701 tgcggctgaa atgactcctc gccagtggtg gctagcagtg agtgaagcac tggccgaaat
     5761 gctgcgtgct cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc
     5821 aatggagttt ttgattggtc gcctgacggg caacaacctg ttgaatctcg gctggtatca
     5881 ggatgtacag gattcgttga aggcttatga catcaatctg acggacctgc tggaagaaga
     5941 gatcgacccg gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgactc
     6001 aatggcaact gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt
     6061 ccgccagtct tttgtcgatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa
     6121 ctacccgtgg ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt
     6181 gacgaaagac ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc
     6241 cgttgtcggc tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc
     6301 gcatccgttt gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg
     6361 catcaatgcg gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa
     6421 aaagctgcgc ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg
     6481 tcgccatcat ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct
     6541 gaacgatacc cacccaacta tcgcgattcc agaactgctg cgcgtgctga tcgatgagca
     6601 ccagatgagc tgggatgacg cttgggccat taccagcaaa actttcgctt acaccaacca
     6661 taccctgatg ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc
     6721 gcgccatatg cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac
     6781 ctggccgggc gatgaaaaag tgtgggccaa actggcggtg gtgcacgata aacaagtgca
     6841 tatggcgaac ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc
     6901 ggatctggtg gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca
     6961 taacgtcacc aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc ctgcactggc
     7021 ggctctgttg gataaatcac tgcaaaaaga gtgggctaac gatctcgatc agctgatcaa
     7081 tctggaaaaa ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc
     7141 gaataaagtc cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca
     7201 ggcgattttc gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct
     7261 gctgcatatt ctggcgttgt acaaagaaat tcgtgaaaac ccgcaggctg atcgcgtacc
     7321 gcgcgtcttc ctcttcggcg cgaaagcggc accgggctac tacctggcga agaatattat
     7381 ctttgcgatc aacaaagtgg ctgacgtgat caacaacgat ccactggttg gcgataagtt
     7441 gaaggtggtg ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc
     7501 ggatatctcc gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa
     7561 actggcgctc aatggtgcgc ttactgtcgg tacgctggat ggtgcgaacg ttgaaatcgc
     7621 cgagaaagtc ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc
     7681 aattctggcc aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc
     7741 agtattgaaa gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca
     7801 gatgctgcac agtatcggca aacaaggcgg cgatccgtat ctggtgatgg cggatttcgc
     7861 agcctatgtt gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac
     7921 tcgcgcggcg atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg
     7981 cgattatcag gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg
     8041 tctggataat gccgcgctgg cggcggggat tagccccaat tacatcaatg cccacggtaa
     8101 accgcagtcg attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac
     8161 cgccacgaaa gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa
     8221 aatgccgatg gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg
     8281 aacacagtac aaaggccatg taacgggggg caaagcgttc aatctaccga cgaagctgcc
     8341 ggaaggttat cacacgctga cactcaccca ggacgaccag cgcgcgcatt gccgggtgat
     8401 tgtcgccccg aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg
     8461 tgcctgcgtt cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg
     8521 cgatctcaaa gcgatgctgg tggatgtggc aaaacgtggc gggtcgttca tcggcctgaa
     8581 cccgattcat gcgctctatc cggcaaatcc ggagagtgcc agcccataca gcccgtcttc
     8641 tcgccgttgg ctgaatgtga tttatatcga cgttaaagcc gtcgaagatt tccatcttag
     8701 cgaagaggct caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaagcgcg
     8761 ggatgccgac tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat
     8821 ggcgtggaaa ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt
     8881 tgcagatcaa ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca
     8941 agtgaaagag gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt
     9001 ggattcacca gaagtgcgtc agttctgcga agaacatcgt aatgacgtcg atttttatct
     9061 ctggttgcag tggctggctt acagccagtt tgccgcctgc tgggagataa gccagggcta
     9121 tgaaatgccg attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga
     9181 aacctggtgt gaccgtgaac tatattgcct gaaagcgtcg gttggcgcgc cgccggatat
     9241 cctcggcccg ttggggcaga actggggatt accgccaatg gacccgcata tcatcaccgc
     9301 gcgtgcctat gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt
     9361 acgaattgac catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc
     9421 agatcagggc gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga
     9481 aagtaaacgt catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat
     9541 tgtcggtaag ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga
     9601 ccacgagaag acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc
     9661 gacacatgac ctgcctacgc tacgcggtta ctgggagagc ggggatctaa cgctgggcaa
     9721 aaccctgggg ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact
     9781 ggcgaagcaa gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg
     9841 gcataaggca tcgttgatgt cgatgacgcc gacgctgaac cgtggtttgc agcgctacat
     9901 tgccgacagt aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga
     9961 accggtgaat attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc
    10021 aacgcttgag tcgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag
    10081 acggcgcaga gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc
    10141 cggcagtcag tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac
    10201 cgagataatg gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa
    10261 gtactctttg aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga
    10321 acccaccgca ataaccatca gctctgggct aacacccgtc gttgcaatca gcggtgccgc
    10381 gataccaccc gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc
    10441 agcaatcgac caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat
    10501 gtatttgtcc acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat
    10561 caacagcatc atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat
    10621 tgaacgacca cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgttgc
    10681 cattaccggg tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat
    10741 catttcggca atcgcacgca tcgccatcag cacaaccggc accagagaag tccagacgct
    10801 gacgccaaag ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg
    10861 gattggctta tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt
    10921 cgggattgcc agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt
    10981 ggcaatcgca gtcgggcccg gatgcggtgg caggaagccg tgggtcacag acagtgcagc
    11041 cgccattggt acaccaacat acagcagtgg aatattcgcg gaagccgcga tggtaaacac
    11101 cagcggcagc atcagcacga agcccacttc atagaacagg gcaaaaccaa cggtaaaacc
    11161 ggttaacacc accgcccact ggatgcgctt tttaccaaat ttggcaatca gcgtggtggc
    11221 gatacgttgt gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat
    11281 gatcagggca aggctaccga gcgtcccgcc gacaccgact ttgatggagt caataacttt
    11341 atccagcggc attccttgca ttaatccaac agcaagcgcc acgaggacga gagcgatgaa
    11401 gccgttcatt ttgaagcgga tcatcaggag caacaacaag attacaccga tagcaacaat
    11461 gactaatggc atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg
    11521 acaaactcta attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac
    11581 taaaactaat tattgtagtc agatgtcagg agtatgtttg gtacccatgt gaatgatacg
    11641 ggtaacattt ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg
    11701 tcatattatc gcggggaaac gaagggaggg atttgccaaa gcaatgctgt gccaacgtct
    11761 ggcacatgtt caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa
    11821 agaggggata acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac
    11881 acctttcagc tccgggaatt cgttcagcag ctgcttctcg atcccttctt tcagcgtcac
    11941 atcgaccatg gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc
    12001 ggtgatttcc atcagcgaaa cgcgaccacc gtgaccagca agctgtgggt tgatctgcga
    12061 ctgcagcata tactccacgc gctccatcag cggtgcatcg tctgccactt tacgcatttt
    12121 ggcgttcggg gctttcagcg ttaactgaga acccaactgg tcggtaacaa agtcgatctc
    12181 tgcatcttcc aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt
    12241 cagggcagtg tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc
    12301 gttaggcgtg ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc
    12361 cagcagtttg gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta
    12421 atagttgact attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac
    12481 aaaggcacca gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg
    12541 cgacggtact tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacgg
    12601 gcaattcaag acgaaaggca tttttcaggt tgcgcttgcg cagccgggca ctgagaaaat
    12661 gctgggtcgc agtggcccgt gtacgtgtga cggcttcgct atcccattgg cagtgcaacc
    12721 agcgtgataa cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc
    12781 gctgccataa cggaacgctg atgatgcgat ccggcaattg caacccggtg gtgcgacgag
    12841 cgtgtaagac ttccaatagt aacagacgtg acagggcgct ggcgatttca ctgcgccggg
    12901 aaaatttaag ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca
    12961 gtctttgcca gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg
    13021 cgggtaatcc acattgtggg cataacgttt tatctgtgcg ggtggcgcgt gaacagaccg
    13081 aacaaatccc ccaatgacct aacgccagtg gcattcggca tagccagcat aatcccggta
    13141 ctgttagcat atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacattt
    13201 ggtggcagac caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga
    13261 atgccgaagt gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcaccttg
    13321 ttgatctgcc cggcttcggg cgcagccagg gatttggtgc gctgtcactt gctgatatgg
    13381 ccgaagccgt gctgcaacag gcacctgata aagccatttg gttaggctgg agtctgggcg
    13441 ggctggtggc aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg
    13501 tggcgtcgtc accttgtttt agtgctcgtg acgattggcc ggggataaaa ccggacgtgc
    13561 tggcgggatt tcagcagcaa ctcagtgatg attttcagcg tacagtggag cggttcctgg
    13621 cgttacaaac catggggact gaaacggcgc gccaggatgc gcgggcgttg aagaaaaccg
    13681 ttctggcgtt accgatgccg gaggttgacg tgcttaatgg cgggctggaa atcctgaaaa
    13741 cggttgatct ccgtcagccg ctgcaaaacg tgcccatgcc gtttttgcga ttgtatggct
    13801 atctcgatgg tctggtgccg cgcaaagtgg tgccgatgct ggataaactc tggcctcgca
    13861 gcaaatcata tatcttcgcc aaagcggccc atgcgccgtt tatttcgcat ccggtcgagt
    13921 tttgtcacct gctggtggcg ttgaagcaga gggtgtaggc aacttttgaa atggcgagac
    13981 agaaatttac tggctcgccg cagccaactc ttcttctgac accccggtaa agcgcatgat
    14041 gtccgcaaga gggactccgg attcaagcat tattttggct atatgcaggg ctttggattg
    14101 ttccccctcc tgccgcaatc tttccgcaat tgtcattaaa ctctccttgt gtttcggtga
    14161 acgttcggca acgccgtcga taaaatcgtt aaaacgtacc gcgtcgccgg tttgcagtat
    14221 gtaattaaac agccctttga tttgtctgtc attagcgtat ccactactta ataagcaggc
    14281 catttgctct accagcccca tcaggt
//
LOCUS       DAZPAC010000101        14103 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZPAC010000101 DAZPAC010000000
VERSION     DAZPAC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14103
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.101"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(9..1367)
                     /gene="hprS"
                     /locus_tag="ACHUPM_002506"
     CDS             complement(9..1367)
                     /gene="hprS"
                     /locus_tag="ACHUPM_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR0870253.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(1367..2038)
                     /gene="hprR"
                     /locus_tag="ACHUPM_002507"
     CDS             complement(1367..2038)
                     /gene="hprR"
                     /locus_tag="ACHUPM_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR0870254.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2171..2584
                     /gene="hiuH"
                     /locus_tag="ACHUPM_002508"
     CDS             2171..2584
                     /gene="hiuH"
                     /locus_tag="ACHUPM_002508"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR0870255.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2693..3697
                     /gene="msrP"
                     /locus_tag="ACHUPM_002509"
     CDS             2693..3697
                     /gene="msrP"
                     /locus_tag="ACHUPM_002509"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFR0870256.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            3698..4333
                     /gene="msrQ"
                     /locus_tag="ACHUPM_002510"
     CDS             3698..4333
                     /gene="msrQ"
                     /locus_tag="ACHUPM_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR0870257.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            4590..5240
                     /gene="zinT"
                     /locus_tag="ACHUPM_002511"
     CDS             4590..5240
                     /gene="zinT"
                     /locus_tag="ACHUPM_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR0870258.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            5583..6113
                     /locus_tag="ACHUPM_002512"
                     /pseudo
     CDS             5583..6113
                     /locus_tag="ACHUPM_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(6683..6772)
                     /locus_tag="ACHUPM_002513"
     tRNA            complement(6683..6772)
                     /locus_tag="ACHUPM_002513"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6736..6738),aa:Ser,seq:cga)
     gene            6866..7663
                     /gene="mtfA"
                     /locus_tag="ACHUPM_002514"
     CDS             6866..7663
                     /gene="mtfA"
                     /locus_tag="ACHUPM_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR0870259.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7764..7839
                     /locus_tag="ACHUPM_002515"
     tRNA            7764..7839
                     /locus_tag="ACHUPM_002515"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7797..7799,aa:Asn,seq:gtt)
     gene            8153..14103
                     /gene="yeeJ"
                     /locus_tag="ACHUPM_002516"
                     /pseudo
     CDS             8153..14103
                     /gene="yeeJ"
                     /locus_tag="ACHUPM_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         3988 a         3353 c         3170 g         3592 t
ORIGIN      
        1 gccctatgtt aatttctttg cggtaacata atccggaaca cattatgctt attgagatag
       61 tgatacgtag cacttccccc atgtaattcg gcaatcgctt tgactaaaga aagaccaagt
      121 ccctgaccta cggaatgacg cgaattatct ccccgccaaa atctacggaa gagtttttca
      181 ggctcatgaa ttttcgttcc aggactggcg acatcaatat taagatagcc gttggtatca
      241 agaaaactgg ttatatgaat acgcgatttt tctggtgaat atctaatggc attaacaata
      301 agattcgata acattcgttg cagtaaaatt ttatccgcaa agatttgctg attgcactcg
      361 accttaaagc aaatctcttt ctcgtctgaa aggtattcaa gataatccaa caaattttcg
      421 acttccttat tgagagaaag cgagtccagt ttcaccaaaa cattgttttt atctgcccgg
      481 gcaagaaaca gtatgttctc tgttaaccgc gaaatatttt ccagctcttc aatgttaccg
      541 gcaattgttt tttgatattc agcgatactt ctggtttgac tgagcgtaac ctgattctga
      601 cccagtaatg cattaatggg cgttctaagt tcatgagcga gatcgtcagc aaattgactt
      661 agacgctcaa aatctttgac taaagcatga tgcattttat tcaacgcctg cccaagaggt
      721 tttagttctc tcggtaatgc gctaacctca acaggctccc ggctatcgtt ataattcagc
      781 gcttccgtta caccactcaa ctttttgatc tctcgtaatc ccgttctgat taacagcgga
      841 cttaatactg agcaaagtac aatggcgaca atgcaaatta taatgctatt aattttatac
      901 tgttcaagca tgttatgtct ggctgaagcc aatttagcca cagtaaccgt taatgatggc
      961 gaaacttcat caatattaat tcgtaaagca tcaatctcag tatcattaat aatgcttctg
     1021 taaatgccag ctgcgctgat tgtctcacta gcaggtatgt tatttaacat gtcatcactg
     1081 acatttgtcc ggttaacaat tttattgatg ccatcaccat gaatgatcaa gatatcctga
     1141 ctaacatcca tcatccggtt aaagtataca ggtaacgtat ctggatttac cccatcaatt
     1201 aacaactgct tgatctgtgc tgtccggtta atgagtgttg tatcatcgcg ccatttcaac
     1261 tcacttgcca ggccattata gagagtccag acaattccag cgccagcaac agacagcagc
     1321 aatataaaaa gcaaggttaa acggacggtt atagataatc ttttcattat ttttttaccg
     1381 ctacgaatga atagcccatc ccccggattg tggcgattag cttttcagga aaaggatcat
     1441 caacttttgc gcggagcctg cgaatggcga catccaccgt attggtatca ctatcaaagt
     1501 tgattcccca aatttcactc gcaataaccg ttctgggtat aatttcgcca gctctggagg
     1561 ccagtagcca aagtaactga aactccttgc gcgtcagtgt aatactgata ttgttcctgc
     1621 tcacactttg acttacagag tccattctta agccactgat ttctaatgtt gaattcaaag
     1681 cgtgatgttg ccttaattgt gcccgaaccc ttgccagcaa ctcagaaaat gaaaaaggtt
     1741 ttaccagata atcatttgct ccactgtcca gccctctgac tctgtcatcg acagaatccc
     1801 ttgcagtaag gcaaataaca ggggtttgct ttgctgttct taacgtttgt aagatctgcc
     1861 agccatccat acccggaagc ataatatcca gaatgatcaa tgcataatca tccttcagcg
     1921 caagataaag cccatctctg ccatcagaaa cggcatcaat gacataaccc gcttcggaaa
     1981 gcccctgcgt tacccactcc tgggtccttt gattatcttc aataagtaaa atcttcatga
     2041 aaataatatg ccataaatca tatgttatag aaacagccta gttcattaca aaattgtaat
     2101 gctgctgtaa ggttaccctg gccgcttttt cgctatcctc aaaactcatt cacatgacaa
     2161 ggatataaac atgttaaagc gttatttagt actctccgta gtaacggcag cattttcatt
     2221 accttctttg gtttatgccg cacaacaaaa cattcttagc gtgcacattt tgaaccagca
     2281 aaccggaaaa cccgctgccg acgtgacagt cactcttgaa aagaaagcgg acaacggctg
     2341 gttacaactt aataccgcca aaacagataa ggatggacga attaaggcac tgtggcccga
     2401 gcaaactgca actacgggcg attaccgtgt cgtatttaaa accggggact atttcaagaa
     2461 acaaaatctt gaaagtttct tccctgagat ccccgttgaa tttcatatta ataaagtgaa
     2521 cgagcattat catgtgcctt tattacttag ccaatatggg tattcaacct atcgtggcag
     2581 ttaatgcata aatatgtaaa agcagatctc tgctactgtt agggaggaca ctccctgaca
     2641 gattaacagt aaacggctct tgctggctaa cgacaaaaaa gtgtgatggc ttatgaaaaa
     2701 gaatcaattt ttaaaagaat cagatgttac ggccgagtcg gtattcttta tgaagcgtcg
     2761 acaggtgtta aaagcactgg gcatcagcgc agctgcactt tctttgcctc acgctgcgca
     2821 tgccgatctg cttagctggt ttaaagggaa cgatcgcccg cccgcccccg ccggaaaacc
     2881 gctggagttc agcaagccta ccgcctggca aaataatctg ccactgacgc cagtagataa
     2941 agtctccggt tataacaact tctatgaatt cgggctggat aaagccgatc ccgccgctaa
     3001 tgctggtagc ctgaaaaccg atccatggac actgaaaatc agcggcgaag tggcaaaacc
     3061 attgaccctc gatcacgatg atttaacccg tcgcttcccg ctggaagagc gtatttatcg
     3121 tatgcgctgc gtggaggcat ggtcgatggt ggtgccgtgg attggttttc cgctgcacaa
     3181 attgctggcg cttgccgaac ccaccagcaa tgcgaagtat gtcgctttcg aaacaattta
     3241 tgcaccggaa caaatgccag gccagcagga ccgctttatc ggcggcgggc tgaaatatcc
     3301 ttatgtcgaa ggattgcgtc tcgacgaagc aatgcatccg ctcacactga tgaccgtggg
     3361 tgtttatggc aaggcgttac cgccacaaaa tggcgcgccg gtacgactga ttgtgccgtg
     3421 gaaatatggc tttaaaggga ttaaatcgat agtcagtatt aagctgaccc gcgagcgtcc
     3481 gccaaccacc tggaatctgg cagcgcctga cgaatacggt ttttacgcca acgttaatcc
     3541 gcatgttgat cacccgcgct ggtcacaggc taccgaacga tttattggtt caggcggcat
     3601 cctcgatgtt cagcgccagc caacgctact gtttaatggt tacgccgacc aggtggcatc
     3661 gctgtatcgt ggcctggatt tgcgggagaa tttctgaatg cgtctgacag caaaacaggt
     3721 gacatggctg aaagttagcc tgcatcttgc cggattgttg ccgtttctct ggctggtctg
     3781 ggcgatcaat cacggtggac tgggtgccga tccggtgaaa gatattcagc attttaccgg
     3841 tcgcactgca ctgaaatttt tgctggcgac cttgttaatc acacctctgg cacgctacgc
     3901 aaaacagccg ttattgatac gcactcgccg cctgttaggc ttatggtgct ttgcctgggc
     3961 gacactgcat ttaaccagtt acgcattgct ggagctgggc gtgaataatc tggcgttatt
     4021 aggcaaggag ttaattaccc ggccttattt aacgttagga attatcagct gggttatttt
     4081 gcttgcttta gcgtttactt caacccaggc gatgcagcga aaactcggca agcactggca
     4141 acagttgcat aatttcgtct atcttgtcgc gatcctggcc ccaatacatt atctgtggtc
     4201 agtgaagatt atttcaccgc agccgctcat ctacgctggg ctggctgtac tgcttttagc
     4261 cttacggtat aagaagttgc gttccctttt taagcggtta cgcaagcagg ttcacaacaa
     4321 attatctgta taacttcata ttgccgacaa agtacgcgtt atccctgccg gatgtggcgc
     4381 gagcgccctg tccggcctgc gcagatcaca tcaggttatg tttcgtttgt catcaacctc
     4441 aaattatcaa cataattcat tttatttcaa taagatagat aagtacacct gagaaaacta
     4501 tgctctcatc tcctaagagt tgttgcattt tgccatatgt tacaatataa cattacacat
     4561 catatacaat gactctggag gaaactgttt tggcaattcg tcttcacaaa ctggctgttg
     4621 ctttaggtgt ctttattgtt agcgctcctg ccttttcgca tggtcatcac tcacacggca
     4681 aacccttaac agaggtcgaa caaaaagctg ctaatggtgt ttttgatgat gccaatgtac
     4741 aaaaccgaac gctcagtgac tgggatggag tctggcaatc cgtttatcct ttactgcaaa
     4801 gtggcaaact tgaccccgtc tttcagaaga aagcggatgc agataaaatt aaaacatttg
     4861 ctgaaattaa agattattat cacaaaggtt atgcaacaga tatcgagatg attggcattg
     4921 aggacggcat tgttgaattc catagaaata atgaaacaac atcctgtaaa tatgattacg
     4981 atggatacaa aatactcacc tataaatcag gcaagaaagg cgttcgttac ttattcgaat
     5041 gtaaagatcc tgaaagcaaa gcccctaaat atatacaatt tagcgatcat ataattgcac
     5101 cacgaaaatc atctcatttt cacattttta tgggtaatga ttcacagcaa tttttattaa
     5161 atgaaatgga aaactggcca acgtattatc catatcagtt gagtagcgaa gaagtggtcg
     5221 aggaaatgat gtctcattga gtattctcat gataacgcct cgatgccgct ttagtaagtc
     5281 atcataactg ccactggtcc atccacaaac gccactgaac gcaagctagc tacagacacg
     5341 ctcatcacta tgacgtgtct gtatattaat aagctaaccc gcattgagtt aaccaataac
     5401 ggattccata cacaatacgg ccaacagaga aagatacctg tgctcacgcc attgcttata
     5461 ttggctggtt acaatgtgca ctatcaattt tttaaataac aaattcttaa aaccacatct
     5521 gtataaggaa tttttaaggt tcgtgggata gcttgactgt aaaaattaca ggagctacaa
     5581 aaatgaaccg attctcaaaa actcaaattt atttacattg gataacgctg cttttcgttg
     5641 caataaccta tgccgcgatg gaactccgtg gctggtttcc taaaggtagt agtacttatc
     5701 tgctgatgcg agaaacacat tacaatgcgg gtatattcgt ttgggtgtta atgttttcac
     5761 gcctgattat aaaacaccgt tatagtgatc cttctattgt accaccgcca cctgcctggc
     5821 aaatgaaagc ggcttcgcta atgcacatca tgctttatat aaccttcctt gcacttcctc
     5881 tgctggggat tgctttgatg gcttacagtg gaaaatcgtg gagtttcctt ggtttcaatg
     5941 tgtctccctt tgttacccca aacagcgaaa ttaaagcact gataaaaaat attcacgaaa
     6001 cctaggcaaa tataggttac tttttaatcg cagctcacgc tggcgcagca ctctttcatc
     6061 actacattca gaaagataat actctgttac gaatgatgca tcgccgcaaa taagtcgatc
     6121 ccctgacaag gaaatgtgaa ccatgtacat caacgcgtca taaatgttta cgcaagtgca
     6181 caatgcatcc tttctactat caacaaggag gagcagaaac tgtgtaaatc gtacaatggt
     6241 aagacttatc aaaaatacag gttaattttg gtatttaaac cttttttact tgttagtgtg
     6301 atacaaaggc tttcaaaaaa gctgggaaat tcaacaactc ttggaaatta tggactttgt
     6361 cccgcggctt taatctgacg gccgcgttcc ttttttgact caattattga ccccttctct
     6421 acgcaacttc agttcccacc accaactttg cggcagtttc ataggatgaa tgtctaaaag
     6481 aataatggtg agcgataaga aaacgactga ataactgcac attttcgctc gaaagcttcc
     6541 tgtcagatcc atagcgaatc aagtgctgaa tgtcacagta tcgaacagaa aacagtgacg
     6601 atctaaccct tcaagaatat tctacaattg ttctgtttag gaaaagcaag gcgggaagtc
     6661 aggagataag tcattgataa agtggcggag agagggggat ttgaaccccc ggtagagttg
     6721 cccctactcc ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat
     6781 ggttgccatg atgccaggaa atttggcatt ttaacagtcc ctgttcgtgc aattttgttc
     6841 aagtgacgag tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc
     6901 acatcaaact gcccttccct ggcaggaagc actatcgatc ccccttttaa cgggtctgac
     6961 ggaacaggaa caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct
     7021 tgttccttta cagggctttg aactggattc attaagaagc tgccggatag cacttctatt
     7081 ttgcctaccc gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat
     7141 ttatcctgcg ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa
     7201 ccaacgtatt gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt
     7261 ggatatacaa gattcttttg atgcatctgg ttttaacctg attattcatg aagtcgctca
     7321 taagctggac acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga
     7381 ggttgctggc tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga
     7441 attggttggc gagaatgcgg cgagcattga tgcttatgca gccagcgatc ctgctgaatg
     7501 ttttgccgta ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc
     7561 ttcattgtgg caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca
     7621 cgctaatgat acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat
     7681 taattaacca attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaagt
     7741 aatatgtgcc ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta
     7801 atccgtatgt cactggttcg agtccagtca gaggagccaa attctaaaaa ttcgcttttt
     7861 tagcgcaatg tcactgacct tagttgaaca ttgtttttta acggatagcg ggtttttaac
     7921 accttaagcg ccctcgacct ttatggttga gggcgttttg ctatgaacgc catcaccatt
     7981 ttcccctcga ttataaaact tgagttattc agtagtctcc cctcttgcaa ctcacaccca
     8041 aaactgccta acgaaaagtt attaattttc aatcgtattg ctatcagtat ttacattttt
     8101 tcgctgtgct agaaagggcg catttatgtt agctcgttca gggaaggtaa gcatggctac
     8161 gaagaagaga agtggagaag aaataaatga ccgacaaata ttatgcggga tgggaattaa
     8221 actacgccgc ttaactgcgg gtatctgtct gataactcaa cttgcgttcc ctatggctgc
     8281 ggcagcacaa ggtgtggtaa acgccgcaac ccaacaacca gttcctgcac aaattgccat
     8341 tgcaaatgcc aatacggtgc cctacaccct tggagcgctg gaatcggccc aaagcgttgc
     8401 cgaacgtttc ggtatttcgg tggctgagtt acgcaaactc aaccagtttc gtacgtttgc
     8461 tcgaggtttt gataatgtcc gccagggtga tgaactggat gtcccggcac aagttagtga
     8521 aaaaaaatta accccgccgc cgggtaatag cagtgacaac ctcgagcaac agatagccag
     8581 tacttcacag caaatcgggt ctctgctcgc cgaagatatg aacagcgagc aagcggcaaa
     8641 tatggcgcgt ggatgggcct cttctcaggc ttcaggcgca atgacagact ggttaagccg
     8701 cttcggtacc gcaagaatca cgctgggcgt ggatgaagat tttagcctga agaactccca
     8761 gttcgatttt ctccatccgt ggtatgaaac gcctgataat ctctttttca gtcagcatac
     8821 tctccatcgt actgacgagc gtacgcagat taacaacggc ttaggttggc gtcatttcac
     8881 tcccacatgg atgtcgggca tcaacttctt tttcgaccac gatcttagcc gttaccactc
     8941 ccgcgtcggc attggcgcgg agtactggcg cgactatcta aaattaagca gtaacggcta
     9001 tttgcgactg accaactggc gcagcgcacc tgaactggac aacgattata aagcacgccc
     9061 ggccaatggc tgggatgtac gcgcagaaag ctggctaccc gcctggccgc accttggcgg
     9121 taaactggtc tatgaacagt attatggcga ttaagtggcc ctgttcgata aagacgatcg
     9181 gcaaagtaat cctcatgcca taaccgctgg acttaactat acccccttcc cgctgatgac
     9241 cttcagcgcg gagcaacgcc agggtaaaca gggcgaaaat gacacccgtt ttgccgtcga
     9301 ttttacctgg caacctggca gcgcaatgca gaaacagctt gacccgaatg aagtcgctgc
     9361 acggcgtagc cttgcaggca gccgttatga tctggtggat cgcaacaaca atatcgttct
     9421 ggaatatcgc aaaaaagaac tggttcgcct aaccctgaca gaccccgtga cagggaagtc
     9481 aggagaagtg aaatcactgg tttcgtcgct acaaaccaaa tatgccctga aaggctataa
     9541 cgtcgaagcc accgcactgg aagctgccgg tggcaaagtg gtcacaacgg gtaaagatat
     9601 tctagttacc ctgccggctt accggttcac cagtacgcca gaaaccgata acacctggcc
     9661 gattgaagtc accgccgaag atgtcaaagg caatttgtcg aatcgtgaac agagcatggt
     9721 ggtcgttcag gcacctacgc taagccagaa agattcctcg gtatcgttaa gtacccaaac
     9781 attgaacgcg gattcccatt caaccgccac actgactttt attgcgcatg atgcagcagg
     9841 taatcctgtt gtcgggctgg tgctctcgac gcgtcacgaa ggtgttcagg acatcaccct
     9901 ttctgactgg aaagataatg gtgacggaag ctatacccag atcctgacca caggagcgat
     9961 gtctggcacg ctgacgctga tgccacagct gaatggtgtg gatgcggcta aagcccccgc
    10021 cgtggtgaat atcatttctg tttcgtcatc ccgaactcac tcgtcaatta agattgataa
    10081 ggaccgttat ctctccggca atcctatcga ggtgacggta gaactgagag atgaaaatga
    10141 caaacctgtt aaggaacaaa aacagcaact gaataacgca gtcagcatcg acaacgtgaa
    10201 accaggagtc actacagact ggaaagaaac cgcagatggc gtctataagg cgacctatac
    10261 cgcctatacc aaaggcagtg gacttactgc gaagctattg gaatgaagat ttgcataccg
    10321 ctggatttat catcgacgcc aacccgcagt cagcgaaaat tgcgacatta tctgccagca
    10381 ataatggtgt actcgccaat gagaatgcag caaacaccgt ctcggtcaat gtcgctgatg
    10441 aaggaagcaa cccaatcaat gatcataccg tcacgtttgc ggtattaagc ggatcggcaa
    10501 cttccttcaa caatcaaaac accgcaaaaa cggatgttaa tggtctggcg acttttgatc
    10561 tgaaaagtag taagcaggaa gacaacacgg ttgaagtcac ccttgaaaat ggcgtgaaac
    10621 aaacgttaat cgtcagtttt gtcggcgact cgagtaccgc gcaggttgat ctgcagaagt
    10681 cgaaaaatga agtggttgct gacggcaatg acagcgccac aatgaccgcg accgtccggg
    10741 atgcaaaagg caacctgctc aatgacgtca tggtcacttt caatgttaat tcagcagagg
    10801 cgaaactgag ccaaaccgaa gtgaatagcc acgacgggat cgccacagct acgctgacca
    10861 gtttgaaaaa tggtgattat agggttacgg cctctgtgag ctctggttcc caggctaatc
    10921 aacaggtgaa ttttatcggt gatcaaagta ctgctgccct gaccctcagt gtgccttcag
    10981 gtgatatcac cgtcaccaac acagctccgc aacatatgac tgcaaccttg caggataaaa
    11041 atggcaaccc actaaaagat aaagaaatca ccttctctgt gccaaacgac gtcgcaagta
    11101 agttctcgat tagcaacgga ggaaaaggca tgacggatag taacggggtt gcaatcgcct
    11161 ccctgaccgg cacgttagcg ggcacgcata tgatcatggc tcgtctggct aacagcaatg
    11221 tcagcgatgc acagccaatg acgtttgtgg cggataaaga cagagcggtt gtcgttttgc
    11281 aaacatcgaa agcggaaatc attgggaatg gcgtggatga gacaactctg acagcaacag
    11341 tgaaagatcc gtcgaatcat ccggtggcgg ggataacggt aaacttcacc atgccacagg
    11401 acgttgcggc aaactttacc cttgaaaata acggtattgc catcactcag gccaatgggg
    11461 aagcgcatgt cacgctgaaa ggtaaaaaag cgggcacgca tacggttacc gcaacgctgg
    11521 gtaataacaa taccagtgat tcgcagccgg taacatttgt ggcggacaaa gcctcggctc
    11581 aggttgtcct gcagatatca aaagatgaga tcacaggtaa tggcgtcgat agcgcaacgc
    11641 taactgcaac ggttaaagat cagttcgaca atgaggtgaa taatcttccg gtaacattca
    11701 gctcagcctc ttcaggactc accctgaccc cgggagtaag taataccaat gagtctggca
    11761 tcgcgcaggc cactctcgca ggcgttgcct ttggtgagca gacggtcact gcatcactgg
    11821 ctaataatgg tgccagcgac aacaaaactg tgcattttat tggcgacaca gcggcggcaa
    11881 aaattatcga gttgacgcct gtcccagaca gcataatcgc cggtaccccg cagaacagct
    11941 ccggcagcgc catcaccgcc acagtcgttg ataataatgg ctttccggtg aaaggtgtga
    12001 ctgtgaactt caccagcaga acaaactctg ccgaaatgac gaatggcggc caagccgtaa
    12061 cgaacgaaca gggtaaggct accgtcactt ataccaatac ccgctcctcg atagaatcag
    12121 gagcgagacc ggataccgtt gaggccagtc tggaaaatgg tagctccacg cttagcacat
    12181 caattaatgt caacgctgat gcgtctacgg cacatctcac ctggctacag gcactttttg
    12241 atacagtctc cgcaggcgag acaaccagtc tgtatattga ggtgaaggat aattacggca
    12301 acggtgtccc ccagcatcag gtgaccctca gcgtttcacc aagtgaaggc gtgaccccca
    12361 gtaataacgc tatatatact accaaccacg acggcaattt ttacgcaagc tttaccgcta
    12421 caaaagccgg ggtttatcaa gtgacggcaa ccctcgaaaa tggcgattcg atgcaacaaa
    12481 cagtgaccta tgtgccgaac gtcacgaatg ccgaaatcac gctggcaacc tcgaaggatc
    12541 cggtgattgc cgacaataac gatctcacga cactaacagc aacagtcgct gatacagagg
    12601 gcaatgcgat agccaacact gaagtaacat ttactctgcc ggaagatgtt aaggcgaact
    12661 tcacgctgag cgatggcggt aaagcgatta ctgatgctga aggcaaagcg aaagtcacgc
    12721 tgaaaggtac aaaagcaggc gctcatactg ttacagcatc gataactggc ggtaagagtg
    12781 agcagttggt ggtgaacttt actgcggata cgctcactgc gcaggttaat cttaacgtta
    12841 ccgaggacaa ttttatcgct aataacgtcg ggatgaccag gctgcaggca acagtgactg
    12901 atggaaacgg caacccgtta gccaatgagg cggtgacatt cacgctaccg gcagatgtga
    12961 gcgcaagctt tactctcgga caaggcggtt ccgccattac tgacatcaac ggcaaggctg
    13021 aagttacact gagcggtaca aaatccggca cctaccccgt gacagttagc gtgaacaatt
    13081 atggtgtcag tgatacgaaa caggtgactt tgattgccga tgctggtacc gcaacactag
    13141 cctccttaac ctctgtatac tcattcgtcg tcagcacgac cgaaggcgcg accatgactg
    13201 caagcgtcac tgacgctaac ggcaacccgg tagaaggcat aaaagttaat ttccgcggaa
    13261 cctccgtcac gctaagcagc accagcgttg aaacggatga tcggggtttc actgaaattc
    13321 ttgtgacaag caccgaagtc ggactgaaaa cagtttcagc ctctctggca gataaaccta
    13381 ctgaagtcat atcgcgatta ctgaatgcaa aagcagatat taattctgca acgattacca
    13441 gtctggagat acctgaaggt caggtcatgg tcgcacaaga cgtagcagtt aaagctcacg
    13501 tcaacgacca gtttggcaac ccgattctta atgaatctgt aacattcagt gcagagccac
    13561 cagagcacat gaccatcagc caaaatattg tctctactga tacgcatggt atagccgagg
    13621 tctccatgac gcccgaaaga aacggttcgt atatggtgaa agcatccctg gcgaatggag
    13681 cctcacttga gaaacaactg gaggctattg atgaaaaact gacactctcg gcgtccagcc
    13741 cgcttatcgg tgtcaattcc ccaacaggtg caactctgac ggcaacgcta acttctgcaa
    13801 atggcactcc agtggagggt caggtcatca actttagcgt aacgccagaa ggtgcgacgt
    13861 taagtggcgg aaaagtgaga actaactctt ctggtcaggc tccagtcgtt ctgaccagca
    13921 ataaagtcgg tacatatacg gtgactgcat cgttccataa cggcgtaaca atacagacac
    13981 agacaaccgt gaaagtcact ggcaactcaa gcaccgccca tgttactagc tttatcgctg
    14041 atccatcgac tatcgccgcc accaacagtg atttaagtac cttaaaggca acggttgagg
    14101 atg
//
LOCUS       DAZPAC010000102        14100 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZPAC010000102 DAZPAC010000000
VERSION     DAZPAC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14100
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.102"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(17..913)
                     /gene="yhaJ"
                     /locus_tag="ACHUPM_002517"
                     /pseudo
     CDS             complement(17..913)
                     /gene="yhaJ"
                     /locus_tag="ACHUPM_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            1018..1719
                     /locus_tag="ACHUPM_002518"
     CDS             1018..1719
                     /locus_tag="ACHUPM_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR0870260.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1742..1906
                     /gene="yhaL"
                     /locus_tag="ACHUPM_002519"
     CDS             1742..1906
                     /gene="yhaL"
                     /locus_tag="ACHUPM_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR0870261.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2040..3350)
                     /gene="cyuA"
                     /locus_tag="ACHUPM_002520"
     CDS             complement(2040..3350)
                     /gene="cyuA"
                     /locus_tag="ACHUPM_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR0870262.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3378..4709)
                     /locus_tag="ACHUPM_002521"
     CDS             complement(3378..4709)
                     /locus_tag="ACHUPM_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0870263.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4984..6348)
                     /gene="tdcG"
                     /locus_tag="ACHUPM_002522"
     CDS             complement(4984..6348)
                     /gene="tdcG"
                     /locus_tag="ACHUPM_002522"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0870264.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6420..6809)
                     /locus_tag="ACHUPM_002523"
     CDS             complement(6420..6809)
                     /locus_tag="ACHUPM_002523"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR0870265.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6823..9117)
                     /gene="tdcE"
                     /locus_tag="ACHUPM_002524"
     CDS             complement(6823..9117)
                     /gene="tdcE"
                     /locus_tag="ACHUPM_002524"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR0870266.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9151..10359)
                     /gene="tdcD"
                     /locus_tag="ACHUPM_002525"
     CDS             complement(9151..10359)
                     /gene="tdcD"
                     /locus_tag="ACHUPM_002525"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR0870267.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10385..11716)
                     /gene="tdcC"
                     /locus_tag="ACHUPM_002526"
     CDS             complement(10385..11716)
                     /gene="tdcC"
                     /locus_tag="ACHUPM_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR0870268.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11738..12727)
                     /gene="tdcB"
                     /locus_tag="ACHUPM_002527"
     CDS             complement(11738..12727)
                     /gene="tdcB"
                     /locus_tag="ACHUPM_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR0870269.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12826..13764)
                     /gene="tdcA"
                     /locus_tag="ACHUPM_002528"
     CDS             complement(12826..13764)
                     /gene="tdcA"
                     /locus_tag="ACHUPM_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR0870270.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3693 a         3781 c         3287 g         3339 t
ORIGIN      
        1 ttaacaataa taaatattat tttccgctaa aaagtttggg aatttcccgc agacaccagg
       61 atttcgcttc ccccatactg tcacgacgcc aggccataat aatatcgatc tcactggtcg
      121 attccgggct gacgacacgc aaccgccctt ccgcaatatc tttttcgacc atcggatacg
      181 gcatcgtcgc cacgccaagc cccgccagta atgcctgacg tttatcttca atcgtgctca
      241 ccgttaagcg cggctgtttg tccagcagtt gtacggtcaa caccgggcgc tcacgagcgg
      301 tatccgctac cgcaattcca cgatatttca cgcgcgtcac ttcagataac ggttccggct
      361 cctgatgaat cgggtgatca ggcgcggcaa cgtagacgtt cattaacgta tagagcttgc
      421 gcgagttgat ctccgacgag gaacgaaaat gcatatccgg cgcgataaca atatccgccc
      481 gcccctgctc cagtcgttcc tacgcccccg ccagcacttc ggtgatgatt gccagttggg
      541 tattggcttt tgccgccagt ttgtcgatta acgggaaaaa ggcaggtgtc ggtaccagcg
      601 cttcggtcac aatggtgaga tgcgtttccc aaccgcgcgc gagggcttcc gcatcggtag
      661 tcagtttatc tgcggcttcc agcaaaacgc gccctcgctc cagcaacatc ctcccgacat
      721 tggtgaattt ggtgcgatgg cccgagcggt caaacagcac cacatccagc tcttcttcca
      781 gtttttgcat ggtgtagcta agtgcggaag gcacgcgtcc cagctcatcc gccgccgccg
      841 caaaactgcc ccggcgatcg atcgcatcca taacccgtag tgcttccagc gttaatgccc
      901 tttctttggc catttcgttc tcattcagga aatttgaaca taccgggcag aatatctggc
      961 taacaatgta gcgtccagcc ccttaccata aaaggaagta aagagaggtc aagaattatg
     1021 attactaccc gaactgccag gcagtgtgga caagcagact acggatggtt gcaggcccgg
     1081 tatacttttt cctttggaca ctacttcgac ccgaaattgt taggctatgc ctccctgcgt
     1141 gtgcttaacc aggaagtgct ggccccaggt gccgcctttc agccgcgaac ctatcccaaa
     1201 gtcgatattt taaatgtgat tctggatgga gaagcagagt atcgcgacag cgaaggcaat
     1261 catgttcagg ccagcgccgg tgaggcgttg ctgctctcta cccagccggg tgtcagctac
     1321 agcgaacaca atctcagcaa agacaaaccg ttaacacgaa tgcagctttg gctggacgcc
     1381 tgcccgcagc gagagaatcc gctgattcaa aaactggcgc ttaatatggg caagcagcaa
     1441 ttaatcgcct cgccagaggg tacgatggga agcctgcaat tacgccagca agtgtggctg
     1501 caccatatcg tgctcgacaa aggcgaaagt gcgaatttcc agttgcatgg gccacgcgcg
     1561 tatttgcaat cgattcacgg gaaatttcat gcgcttacgc atcatgaaga gaaagcggcg
     1621 ctgacctgcg gtgatggggc gtttattcgt gacgaggcta acattacgct ggttgccgat
     1681 tccccactgc gcgctttgct gatagatttg cctgtctagt tgtttttaca gggagatgat
     1741 gatgagtaaa aaatcggcca aaaagcgcca gccagtgaag cccgtggtgg cgaaagaacc
     1801 tgctcgcacc gccaaaaatt ttggctatga agagatgttg agcgagctgg aagccatcgt
     1861 cgcggatgct gaaacgcgtt tagccgagga tgaagctacc gcgtaaagac aggttgccgg
     1921 atgcggcgaa aacgccttat ccggcctaca aaatccaaca acattaacga attaaacaac
     1981 ccgtaggccg gataaggtgc gccagcatcg catccggcac aatactcaaa acctggcgtt
     2041 tatctggcct tgctcgccat aatctcgata atctgccgat ccgtttgctg catcgaatgg
     2101 cttgctaacg cacacaggtt ggcaatcgac tgctcaacat catgcgccac gatcccttca
     2161 ttgccggtca cggcggtatc atccagcgcc attaacaccg ctttccacgc agccgaagca
     2221 ctggtcgaaa ccttcatcgc gcagctgttc gacgcaccat cgcaaatcat gccgctgaca
     2281 tcgccgatca tactgctgat cgccatcgag atagtttcat aacgcccatc caccagccat
     2341 gccatcccgg cggcggcccc cattgctgcg gtcgttgcgg cacaaagtgc agacaaacgc
     2401 ggtaactggt tatggatgta aattgcgctc aaatgagaaa gcatcagcgc acgcgccagt
     2461 cgttcatcat ccgctccgaa gtgttctgct acaaccacca caggcattgt tgcggtgatc
     2521 ccctggttac ccgagccgga gttactcata gccggaagcg tagcgccgcc catacgcgca
     2581 tcggatgccg cgctggtacg aatcacaatg gatgaagaga gatctttcgc cagcaaaccg
     2641 cgctcgcact gtttttccag cgtcgcgcca atatgcagcc cccacttacc gctcaaacct
     2701 tcctgcgata acgcacaatt tagcttcgcg gaatcgagaa taaagcggat cgccgcaaac
     2761 gggacttcat tgacgaactt caggatctca gccagcgtcg ttctggaaag caccgtcagc
     2821 ggagactctt gctcgccctc tgccacacac gcctgctggg taaacaccac accattgtgc
     2881 gtctcaatac gcacaatgtt ggtatgcccg ccgacgatgg tgacacacgc ccacttctca
     2941 ccgttccaga ctttggcgcg tgagaagagg atttcattgc aaggttcctg gatcttaacg
     3001 gagactttcc ccgccgccag cagtgctttg gcatcggcaa ttgcctgcgc agttgcgtct
     3061 ttcagcactt ccagcccggc gttggcattt ccacctaacg cccccagcgc cgccgcaatc
     3121 ggcagcccca ccattcccgt gccgggaacg gtgacgccca gaccgttctt catcagattt
     3181 ggcgaaaccc aggcttctac acgttcaacc ggaccttcca gttctgccgc agcaaccgcc
     3241 gccgccagcg ccagtgaaat cggttcagta catcccagcg ccggttttac ttcctcctga
     3301 acggcgagga tgtaacgctg ccataacgga tttaaagtcg aatcaaacat attaaaaacc
     3361 ttaaaatttc aggtaaatca agaaaatgcc aggaacggag aaacacaaag caacaaacca
     3421 gtgacgataa tcaggtacag agacatccct ttgtatttgt gcaatgccgg tactttgtaa
     3481 accagccacg ccgggatcag gcaccctacc atgccgaaaa tcgggctaca gatagaggtg
     3541 aagctcaaca ccggtgcgtt cagtacgata gcgctccagg ccagcaaaat ggcgaaaatc
     3601 atgatgccgc gctgaacaag attttcgtta atcttctcgg caggcatctt gcgacgcagg
     3661 atgttcatta cgatcccttg cgttgcttcg cgaaagccta aatagacgcc aaagaatgca
     3721 gtcatgacag caaagatatt gagaatgacg ctgaccactt tcacccacgc tgcgccgtca
     3781 ccgctaataa actgtgcggc aatcgccagc gcggaaatat tctgctcgta ggcttttacc
     3841 gcttcgtcat gccccatcgc cagcgtgaac gacacggcgt agaaaaagac ggtgacaaac
     3901 aaaatgccaa acgcgatatt cattgcccgc aatgctttat gccgcgccac ttcaatagat
     3961 ttttcccgcg agcgataaga gatcaccatc gggcttaacg tctggataaa caaaatcgac
     4021 gtcagggtaa acggcagcgt aataatggcg tttttcacca gcagccccag cggcggtagc
     4081 gaaccgacgt tatacagatg ccacattccc accatcgaca cgcccagcgc cgcgaccacc
     4141 agcagcttgg tcagcaccat gccggtcgaa attttgaata acaatttctc gccgcgtgaa
     4201 gagatcgcca ccagaatgca aatcagtacc agaccataaa agggactgtc tgacagcaac
     4261 ccttccgtca cgccgaaggt atgcagatag gaagcactat cgttggtgat ggcggtggaa
     4321 tagacgaaca tccaaatcac cagcattacg aaatagagcg cacctaacag gatgccccag
     4381 tttttaccta aataaccgct aatgacactc gggtaatctt tacattctgg tgattctgcc
     4441 agcgtattaa taaacaaccg ctgaaacaga tacattgccg ggtaaccaat caccgatgag
     4501 agcaaaaata cccacaatcc catcaaaccg acctgcaccg ggagaaaaac aatccccgcg
     4561 ccaatcgcca tcccgatact cataatcacc cagccggtgt cagtactgtc gaatttgatc
     4621 gcttctcgcc actcgctctc actcattcca gcacgacccg ccggggtcga agcgtctgca
     4681 atgacgcctt tattcgatgc gatttccata atttctcgct caatattttg tagggcttat
     4741 tatttttttc cgagccgcat caaggcgata tgcggtctac gtttttgcag gcgagttatt
     4801 agaataaaga aatgatacgc gcgaggtcag agaaaatctt cacaatcaag actttgaaat
     4861 aacggagatg gataagaatt ttctactaaa ttaatcgcaa gagaatattc attctctatt
     4921 tgcggcagat cacaaaaaaa aggtgcacat ttgtgcaccc aaggatgaaa gctgacagca
     4981 atgtcagccg cagaccactt taatggccag tcctccgcgt gatgtttcgc ggtatttatc
     5041 gttcatatct ttgccggttt catacatcgt ctcgatcact ttatcgagtg aaacacgcgg
     5101 tgccgaggtg cggcgcatcg ccatccacgc ggcgtttact gctttcacgg cattaatggc
     5161 attacgttca atgcacggga tttgtacctg tccggcaacc ggatcgcagg tcagcccaag
     5221 gttatgctcc atcgcgattt ccgccgcatt gcatacctgc gccggactgc cgcccagtag
     5281 ttcagttaac cctgccgccg ccattgaaca ggccacgcca atctccccct gacagccgac
     5341 ctccgcgcca gagatggagg cgttcatttt atacagcgcg ccaatagccc ccgctgccag
     5401 aaaatagcgg gcaattgacc gctcgtttac cggacgacgg aacttatcgt aataagccag
     5461 cactgccgga ataatgccgc acgcaccgtt agtcggtgcc gttaccacgc gcccgccagc
     5521 tgcgttttct tcactaaccg ccagcgcgta catgttgatc cagtcgatga cattcatcgg
     5581 atcgttagag atgttatcgc tagaaaccag ctgacgacgc agcgctacgg cacgacgcgg
     5641 cacattgagc ggaccaggca gcacgccttc agtgttcatc ccgcgttcaa taccgtcatg
     5701 catcacttgc cagatacggg caaatcctgc gtcaatttcc gctttgctgc gcagcgccag
     5761 ctcgttgtgc atcatcaggc cagatataga caggccgttg taatcacaca ttttcagcag
     5821 ttcacctgct gagtggaaat cgtaaggtac ggacgtttcg acatcgtgcg acaggccgaa
     5881 gtgttcttct tcgacaataa acccgccgcc gacagagtaa taggttttac ttaatagcgc
     5941 ttcctgtccc ttccaggcag tgatccgcat tccgttctca tggcgaggca acatttcggg
     6001 atggaagata atgttctttg ctacaggaaa atcaacaata tgcgcaccag atgccactgg
     6061 cagccgcccg ctgcgcgtta ctaactctat aaatgcaggg atctcatcaa tgacaacatc
     6121 ctgcggactg tttcctgcca gtcccatgat aatggcgaca tccgtggcat ggcctttgcc
     6181 cgtcagtgac aacgacccat acagatcgac cacaatatgg ctcgtcgcgg ttaataagcc
     6241 gctactttcc agccgatcaa taaaactttt tccggcattc attggcccca cggtatgcga
     6301 actggaggga ccaatcccaa ttttgaaaat atcgaatgca ctaatcatat ccacaccctc
     6361 ggattgccgt tcagtgaagt ggagcggaac gaccttgcga ccgtcccgct cacgaggctt
     6421 tacgcactac gtactgcgat agcttcaatt tccagcttca catctttcgg caaacgcgca
     6481 acctgcacac agctccgggt cgggtaggtc gcctgatgct catcgaagaa ctgcttatag
     6541 acttcattga tggtggcaaa atcattcaga tcggtgatga acacggtcat cttgatgata
     6601 tcgcccacgc tcagcccggc agcaaccacg atcgctttga cgttttcgag gcttaaacac
     6661 gcctgatctt gcacatcagc cgggatctca ccggtctgcg ggcaaaccgg aatttgcccg
     6721 gaggtgaaga ccatgctgcc taaatcaacg ccctgaacat aagggccgat tgcgcctggg
     6781 gcacgttgcg tttcgataat ctttttcata catcctccgg cgtcagagcg cctgggtaaa
     6841 agtacgtgaa ataacatcct gttgctgttc acgggtcagt gcgttgaagc gcacggcgta
     6901 gccagagaca cggattgtca ggttaggata tttttccggg tgctcgatgg catccagcag
     6961 catttcccga ttcattacgt tgacgttgag gtgttgaccg ccttcgacat ccgcttcgtg
     7021 gtggaaatac ccatccagca ggccgacaag gttggtttta cgtactggat cttctttgcc
     7081 cagcgccgca ggaacgattg agaaggtgta cgagatccca tctttggcgt aggtgaacgg
     7141 cagtttcgcc accgacgtca atgaggccac ggcacctttg cggtcacgac catgcatcgg
     7201 gttagcgccc ggcgcgaacg gtgttccggc gcgacgaccg tccggcgtat taccggtttt
     7261 ctggccgtac accacgttag aagtgatagt cagaatcgac tgggtaggga cggcgttgcg
     7321 ataggttggc agcgctttaa ttttcttcat aaagcgttca accaggtcgc aggcaatgct
     7381 gtctacgcgc tcgtcgttgt tgccgtactg cggatattca ccgtcgattt caaagtccac
     7441 cgccaggccg ttttcgtcac ggattggttt cacgcgggca tatttgatgg cagacaggga
     7501 gtccgtcgcc accgacaggc ccgcgatgcc gcatgccata gtgcgataga catcacgatc
     7561 gtgcagcgcc atcagcgaag cttcgtagct gtacttgtcg tgcatgtagt gaatgatatt
     7621 cagcgcgctg atgtactgca ccgccagcca gtccatgaag tgatcgaggc tgtccatcac
     7681 tttgtcgtaa tccagcacgt cgtccatcag cggtgctgtt ttcggcccga cctgaatctt
     7741 cagcttctcg tccaccccgc cgttaattgc gtacagcagt gttttcgcca ggttagcgcg
     7801 tgcaccaaag aactgcattt gcttaccaat caccattggg ctgacgcaac aagcaattgc
     7861 gtaatcgtcg ctgttgaagt cagtacgcat cagatcgtcg ttttcatact gcaaggaaga
     7921 ggtgacgatc gacacctgcg cggcatattt tttgaaggca atcggtaatt cttccgacca
     7981 aagaatggtc aggttaggtt ccggtgccgg ccccatagtg tgcagggtgt gcaaatagcg
     8041 gaaggagttt ttggtcacca gcgtacgacc gtccagcccc atcccgccga tcacttccgt
     8101 cgcccagatt gggtcgccgg agaacagcga atcaaattcc ggcgtacgca agaagcgcac
     8161 catacggatc ttcataatga agtgatcgat cagttcctgt gcctgctgct cattgagtac
     8221 gcctgcttta aagtcgcgct caatgtagat atcgaggaac gacgccgtgc gccccagcga
     8281 catcgcgccg ccgttctgtg atttcactgc cgccagataa gcgaagtaga gccactgcac
     8341 cgcttcctgc gcattctgcg ccgggcgaga gatatcaaag ccatatttcg ccgccatttc
     8401 ctgaatctgc aacagcgcat gacgatgctc tgccagctcc tcacgcagac ggatggtggc
     8461 ttccagatct tcgccttttt ccagacggga ctggagatcg gcaaattgca gttcgcgttc
     8521 acgcaccaga taactgatgc catacagcgc tacgcggcga tagtcaccga taatgcgccc
     8581 acggccatag ccatctggta aaccggtcag cacgccagat ttacggcagc gcagcatatc
     8641 cggtgagtaa acatcaaata cgccctggtt atgggtttta cgcagatcgg taaacagata
     8701 ttcaaattca ctgtccattt cacggccata ggcgtggaat gaacttttaa tcatattaat
     8761 gccaccgaac ggatgcagcg cgcgctttaa tggcgcatcc gtttgcagac caacaatttt
     8821 ttccagcggc tggttaatat atcccgcatc atgagcggta attgtggtgg caatattggt
     8881 atcgaaatca accggcgcgt gggttgcgtt ttcgatacgg atgccttcca ttactttttc
     8941 ccacaattcc gtggtggcag gcgtcgcttc ggcgaggaaa gattcatcgc cttcatacgg
     9001 tgtatagtta tgttgaataa aatcgcggac attaatttcg tttttccagt ccgtaccttt
     9061 aaagccaagc catgcgtcgg cgtacagctt atcgctggta tcaatatcta tcttcatgaa
     9121 aaataatctc tctacaatac ttcaactaaa ttatgcaaat tctgcgggcg cgttaacttt
     9181 gcctaaatga atggcatcca aagcaatcat tttttcttcg ttagtcggaa taacggcaca
     9241 aatgacacgc gcattttcac tggaaacaat tcgctctcca aaagagttag agcgattatt
     9301 catttctgta tcaatcacta cgcctaatac agccaaatgt tccatgacca gacgacgaat
     9361 taaacttgaa ttctctccta ttccgccggt gaatataatt ccatccaggc gacgtaatga
     9421 agctgcgtgt ccggcaatat gacgggcaat tcggtgaaca aaggttttaa ttgccagttg
     9481 cgcgcgttcg tgaccttcat gccaggcttt ttccagaaca cgtaaatccg aagaaagacc
     9541 ggaaatacct aataatcccg actctttatt cactacgcgt tccaggtcac ccaggctctg
     9601 gttggtttgg ctggcgaccc aggacatcgc accaaagtcg acatcgccac tgcgggttcc
     9661 catcatcaag ccttccagcg gcgtcattcc cattgaagta tcaacactct gaccgttgcg
     9721 aaccgcgcag attgacgcgc cattgccaag atgcgccaca accaggccgg agtcatcttc
     9781 cgccagattc agcagagaat gtgcgcgctg ggaaacatag cggtgcgacg tgccgtggaa
     9841 accataacgg cgtacaccta actcttcata atatttccac ggcaggccgt ataaataagc
     9901 ttccggagcc atcgtctggt ggaaactggt atcaaatacc gccacctgag ttacgcccgg
     9961 aaataattgc tgcgccgatt caataccact taaattggcg taattatgca ggggtgccag
    10021 tggagaaacg cgacggatat tatcaatgac ttcatcggta ataatggcgg actcggtaaa
    10081 aatactgccg ccgtgagcga tgcggtggcc aattaaggcc acactgtcat ttaaattccg
    10141 tttttccagt tcaaatgcaa ttgccttcaa tgcaccttcg tagctgtggt gagccagcgg
    10201 tgctggctct cccccattta cggataagaa tgcattttcc gagttaatac cgtcggcaat
    10261 acctgacatt aatacttcac agtcgctggc atcgagcacg gaaaacttaa tcgaagacga
    10321 accacagtta ataaccaaaa caaccggaaa ttcattcatc tcttttctca tcctgagtta
    10381 cggattaaaa cagtttgtat acgatgttca ggatggtcag cagaccaatc acggtaacaa
    10441 acacgttatc cagacgacca cggtatttcg ccagagacgg cgctttacgg atggcataca
    10501 tcggcaacag gcacagcagg gatgcgataa tcggtgcgcc catggcttca atcaggtcga
    10561 ggatgttcgg gttggcgtag gcaacaaccc aggtggagcc catgatgaag atcatgctga
    10621 tagtgttcag tttacccagc gacactttag ttttgtcgcc tttataacca aacttcagga
    10681 ccaggccatt cagaccttcc agcgttccca gatagtgacc gaagaaagat ttgaagatag
    10741 ccacgagtgc gatgatggaa gccgcatatt ccagtgtaat cgcgaacgtt gttttggtac
    10801 cggtcatgga cgcaaagtgg ttagccagat aagaaagcac tggaatattc tgcgctttgg
    10861 cttccgccat gttggccgga gacagcgtaa ataggcagct aaaggcaaag aacatcacca
    10921 ctgcaaccat cagcatgctg gcacgagaaa tgatttggga acatttacgt tcggtgaagt
    10981 cgcgaccgaa gtctttctca tactcttcac gtttagaaac cacgaaggaa gagacgattg
    11041 gcgagaaatt aaaggagaaa accatgatgg aaatccccag ccagacggtg atcaggatac
    11101 cgtcatgacc ggttaacgac agcgaaccga ggtcaacctg gtcgataact gcagagttcc
    11161 agtaagggat cagcgacaga gaaatcagca ccaggctggc gataaacggc cataccaggt
    11221 agctcatcac tttaaccatc agatccttac caaaccagat gacgaaagcc atcagcagca
    11281 gcaggaacag cgccacaaag ccgcgattca gcggtgcaaa gccgagctgg ttttcccaga
    11341 acgtcataaa ggtattggta atagtaacgc cataaatcca cagcagtggg caaatcgcga
    11401 agaagtacag gaacgtgata accacgccgc cagttttacc aaaatgctct tccaccgttt
    11461 ccgtaatgtt gccggaaggg ttagagccag aaagacatag acgcgccagc gcccggtggc
    11521 aataaaatgc gatggggtat gccaatacca acatcagaag aatcgggatc agtccgccaa
    11581 aacctgcgcg gatagggaag aacagcaccc cggcgccgat tgccgtacca aacaagccta
    11641 acgtccatgt ggtatctgat ttacgccagg acgattgttt tgtctggctg gatacaatgc
    11701 tatctgaagt actcatatcc tatcctcaac gaattaatta agcgtcaacg aaaccggtga
    11761 tttgagagac gcgagaaaga tcgatattgc cgccggaaat aatactgacg gtttttctgt
    11821 tttgaatata ttggtctaat ttaccgctta ataatgcagc acatgccaga gcgcctgcgc
    11881 cttcggtgac gactttattg cgctgaatta aggcaatcat actgtttctg atttcgtctt
    11941 cgctgaccag cacgatgtca tcgactaatt cacgaacgat ttcgtaagtt aaattacccg
    12001 ggcgggagac atcacaacca tccgccaggg tgccggtagt tcggtgcgtg gttatttctc
    12061 cggagtggaa agaagccgcc atgccgtgaa cgttttcaga ctgtacgcca ataacacgaa
    12121 tggtcgggtt aatagattta attgccaccg caataccagc aattaaaccg ccaccaccaa
    12181 ttggcacaat cacgttatcg acatcataga gatcttccat aatttccaga ccaatcgttc
    12241 cctggccagc aatcactttc ggatcatcgt aaggtgggat aaaaatacgg ccttccattt
    12301 cgacaatttc gctcacttta gcgatagtgt cgttgaagtt atcaccatgc agaacgactt
    12361 ctgcggagta gtcgcacgtt gccgctactt tggattttgg cgcacctttt ggcatcacca
    12421 ctttaccgtc gatacccagc atcgcgcagg agagcgaaac cccttgcgca tggttgcccg
    12481 cagaacaggc caccacgcct ttgcgttttt ccgcatcggt cagtgaactt aatttattaa
    12541 atgcgccacg aattttaaat gaacccgtac gctgcatatt ttcaaacttc aggaatattt
    12601 cacctttgca acgttcacta aaatagttgg agcgaggcat gcctgtttta taaattcgcc
    12661 cagccagtcg ttgtttcgct tcaataatat catcaatagc aatcggcaga tcgtatgtaa
    12721 tatgcattat aaaacctctt cgcctgaatt aaatatgtag gtaaccgtaa ccgacacctg
    12781 caaaacagac aggtggatta tttatgttta aataaaaaca aataactaac caacttctat
    12841 taattgcctt cgtctacacc cattataaga tgaatactct ttggctaatt ccaccaaaac
    12901 cgatgctgct tttttaatac gataattttt cgaccatacc gcggcatatt gtgccacagg
    12961 taatgtttct tcaaccggaa tagtaataaa ttgattagaa ccaaaaggtg acgtcatatc
    13021 acaaggaatt acagttaaga aatcagcatt gagaacaaga ttataaattg tcacgactga
    13081 gtcggtttta acgatgtttt caatactgat gccatttctt tgtaacgtag taagcagttc
    13141 gctgtagtac cccatattag tttgtggcaa cacccactgt tcgttcttca acgactccag
    13201 cgtggtggtg ccggtgcatg ttcgggactt actggctacc agcacaaact cggactcgaa
    13261 cagcggctca acatgtaaat cctgaagctt catttctgca cttaatgtac caatcgcaaa
    13321 atccagtcga ccgtcgcgga ttgccggtaa gaacgaagac agttgcgctt catacataga
    13381 aacctgcgct ttcgggaaca cctctttgaa cttgttgatc atccctgaca taaaagtaaa
    13441 accaatcaat gaaggaaaac caaatgagac ttccaccacc gcctcagaag acataccgct
    13501 tatctcatta accatatttt tcatttcacg ggtaatggat tcggaacggg agagtaacaa
    13561 ttgaccggca ggtgttaatg ttacaccggt atttttccgc accactaatt ccacaccaaa
    13621 ataatcttca atatcgttaa tgattttact gacggccggt tgagttaacc ctaattcttt
    13681 tgcagccgag ccgatagagc cacttctaat gacttcctga aagactacca ggtgctgcgt
    13741 tttcggaaga agaatagtgc tcataacgac ctacgttaat tacctcattg acggcatgaa
    13801 gtgtatcaaa atgaaatgaa caggatatgt gcgaccactc acaaattaac tttcaatact
    13861 ttccagggta tcgtcattac aattaaatac ctttaaatat caacaagtta aagcataaaa
    13921 atcagcataa accctgattt ttgtcaaaaa aaatgacggg gataaccata ttttatggcg
    13981 ataacatcat tcgttatgtg gttaatacaa aaaaggggct gagaccatat ttcaagcaat
    14041 taccggataa ttaccaggca aaatttgagt taaatttaat gagcaagttc tcaaatttta
//
LOCUS       DAZPAC010000103        14091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZPAC010000103 DAZPAC010000000
VERSION     DAZPAC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.103"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="ACHUPM_002529"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="ACHUPM_002529"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR0870271.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="ACHUPM_002530"
     CDS             2273..2662
                     /locus_tag="ACHUPM_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0870272.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="ACHUPM_002531"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="ACHUPM_002531"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR0870273.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHUPM_002532"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHUPM_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR0870274.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="ACHUPM_002533"
     CDS             complement(4321..4521)
                     /locus_tag="ACHUPM_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR0870275.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="ACHUPM_002534"
     CDS             4687..5232
                     /locus_tag="ACHUPM_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR0870276.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="ACHUPM_002535"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="ACHUPM_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHUPM_002536"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHUPM_002536"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR0870277.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="ACHUPM_002537"
     CDS             complement(6529..7353)
                     /locus_tag="ACHUPM_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR0870278.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHUPM_002538"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHUPM_002538"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR0870279.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHUPM_002539"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHUPM_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR0870280.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHUPM_002540"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHUPM_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR0870281.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHUPM_002541"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHUPM_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR0870282.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /locus_tag="ACHUPM_002542"
     CDS             complement(11316..11969)
                     /locus_tag="ACHUPM_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR0870283.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHUPM_002543"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHUPM_002543"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR0870284.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHUPM_002544"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHUPM_002544"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR0870285.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3433 a         3562 c         3737 g         3359 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagatggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    14041 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg a
//
LOCUS       DAZPAC010000104        13807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZPAC010000104 DAZPAC010000000
VERSION     DAZPAC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.104"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(2..760)
                     /gene="dsbG"
                     /locus_tag="ACHUPM_002545"
     CDS             complement(2..760)
                     /gene="dsbG"
                     /locus_tag="ACHUPM_002545"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR0870286.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGVMTPTY"
     gene            1132..1695
                     /gene="ahpC"
                     /locus_tag="ACHUPM_002546"
     CDS             1132..1695
                     /gene="ahpC"
                     /locus_tag="ACHUPM_002546"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR0870287.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1866..3431
                     /gene="ahpF"
                     /locus_tag="ACHUPM_002547"
     CDS             1866..3431
                     /gene="ahpF"
                     /locus_tag="ACHUPM_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR0870288.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3552..3980)
                     /gene="uspG"
                     /locus_tag="ACHUPM_002548"
     CDS             complement(3552..3980)
                     /gene="uspG"
                     /locus_tag="ACHUPM_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR0870289.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4201..5439
                     /locus_tag="ACHUPM_002549"
                     /pseudo
     CDS             4201..5439
                     /locus_tag="ACHUPM_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            complement(5443..5532)
                     /gene="yldA"
                     /locus_tag="ACHUPM_002550"
     CDS             complement(5443..5532)
                     /gene="yldA"
                     /locus_tag="ACHUPM_002550"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR0870290.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5670..6080)
                     /gene="rnk"
                     /locus_tag="ACHUPM_002551"
     CDS             complement(5670..6080)
                     /gene="rnk"
                     /locus_tag="ACHUPM_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR0870291.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6310..7116)
                     /gene="rna"
                     /locus_tag="ACHUPM_002552"
     CDS             complement(6310..7116)
                     /gene="rna"
                     /locus_tag="ACHUPM_002552"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR0870292.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7230..8693)
                     /gene="citT"
                     /locus_tag="ACHUPM_002553"
     CDS             complement(7230..8693)
                     /gene="citT"
                     /locus_tag="ACHUPM_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFR0870293.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8744..9622)
                     /gene="citG"
                     /locus_tag="ACHUPM_002554"
     CDS             complement(8744..9622)
                     /gene="citG"
                     /locus_tag="ACHUPM_002554"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR0870294.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9597..10148)
                     /gene="citX"
                     /locus_tag="ACHUPM_002555"
     CDS             complement(9597..10148)
                     /gene="citX"
                     /locus_tag="ACHUPM_002555"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFR0870295.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10152..11683)
                     /gene="citF"
                     /locus_tag="ACHUPM_002556"
                     /pseudo
     CDS             complement(10152..11683)
                     /gene="citF"
                     /locus_tag="ACHUPM_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
     gene            complement(11694..12602)
                     /gene="citE"
                     /locus_tag="ACHUPM_002557"
     CDS             complement(11694..12602)
                     /gene="citE"
                     /locus_tag="ACHUPM_002557"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR0870296.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12599..12895)
                     /gene="citD"
                     /locus_tag="ACHUPM_002558"
     CDS             complement(12599..12895)
                     /gene="citD"
                     /locus_tag="ACHUPM_002558"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR0870297.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12910..>13807)
                     /gene="citC"
                     /locus_tag="ACHUPM_002559"
     CDS             complement(12910..>13807)
                     /gene="citC"
                     /locus_tag="ACHUPM_002559"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR0870298.1"
                     /translation="AGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTK
                     TEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANP
                     FTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEY
                     IISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYN
                     QDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTL
                     HYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3390 a         3497 c         3673 g         3247 t
ORIGIN      
        1 atcaataagt tggagtcatt acccccataa tgatattaag cgttttctga tcgggcaacc
       61 ccacggcctg ttgcagcgta ttttccttac tcatgtaata gatagccggc gtgacatttg
      121 cccccagatc gtccatcagt ttctcattgt cacttaacac tttcatttgc tctgtactta
      181 cgtttgcagg cacgtttagc ttaagcttgc caccagaggc ttcatattgt tgccaggttt
      241 ttgcgggatc tttggaggca agaattgccg ctgctgtcgc cgggctttct ggcttgataa
      301 ccccaaccaa caatgttctt aattgcactt tgccagaatc tacccacggg cgcgcctgct
      361 gccagaactg tttacaatat gggcagaacg gatcggcgaa gacgtagaca atgaccggcg
      421 catctttttt accgtcgagg atccagtggg attgttccat ccgttgccac atttcgcgtc
      481 cggctggtgc gtaaatttct ttttcgataa gtgtgttact caggttttca cctttctcgt
      541 tgtacatgta accagagata gcgtgcttac catcgggagt caggtagatg gtgacgccca
      601 tatcctgata ctttccgaga taacctttca ttcctccggg ggcatcgaac gttttgatga
      661 ttgtaatgcc ctgtttttca atcgctttta ctggggaagg aagttcctct gcgaaggcga
      721 ttgcaggaag cagagccagt aaaagtatct tttttaacat taatttgtcc ttttcagtca
      781 gtgcaaaagt cgagtaaaag gcataaccta tcactgtcat aggtaagagc ttagatcagg
      841 tgattgccct ttgtttatga gggtgttgta atccatgtcg ttgttgcatt tgtaagggca
      901 acacctcagc ctgcaggcag gcgctgaaga taccaaaggg tagttcagat tacacggtca
      961 cctggaaagg gggccatttt actttttatc gccgctggcg gtgcaaagtt cacaaagttg
     1021 tcttacgaag gttgtaaggt aaaacttatc gatttgataa tggaaacgca ttagccgaat
     1081 cggcaaaaat tggttacctt acatctcatc gaaaacacgg aggaagtata gatgtccttg
     1141 attaacacca aaattaaacc ttttaaaaac caggcattca aaaacggcga attcatcgaa
     1201 atcaccgaaa aagataccga aggccgctgg agcgtcttct tcttctaccc ggctgacttt
     1261 actttcgtat gcccgaccga actgggtgac gttgctgacc actacgaaga actgcagaaa
     1321 ctgggcgtag acgtatacgc agtatctacc gatactcact tcacccacaa agcatggcac
     1381 agcagctctg aaaccatcgc taaaatcaaa tatgcgatga tcggcgaccc gactggcgcc
     1441 ctgacccgta acttcgacaa catgcgtgaa gatgaaggtc tggctgaccg tgcgaccttc
     1501 gttgttgacc cgcagggtat catccaggca atcgaagtta ccgctgaagg cattggccgt
     1561 gacgcgtctg atctgctgcg taaaatcaaa gcagctcagt acgtagcttc tcacccaggt
     1621 gaagtttgcc cggctaaatg gaaagaaggt gaagcaactc tggctccgtc tctggacctg
     1681 gttggtaaaa tctaaatttc cttcgtcttt cgcgctacca gctccggcgc gaaatacttc
     1741 ggaaattcac ctaattcttc gggtgctgcg gcacccgatt ttcttccccg caccatgatg
     1801 caagctgcat ccaggtagcc gtagaggccg cttgcatgat gatgtttaaa gcccaggaga
     1861 taaacatgct cgacacaaat atgaaaactc aactcaaggc ttaccttgag aaattgacca
     1921 agcctgttga gttaattgcc acgctggatg acagcgctaa atcggcagaa atcaaggaac
     1981 tgttggctga aatcgcagaa ctgtcagaca aagtcacctt taaagaagat aacagcttgc
     2041 cggtgcgtaa gccgtctttc ctgatcacca acccaggttc caaccagggg ccacgttttg
     2101 caggctcccc gctgggccac gagttcacct cgctggtact ggcgttgctg tggaccggtg
     2161 gtcatccgtc gaaagaagcg cagtctctgc tggagcagat tcgccatatt gacggtgatt
     2221 ttgaattcga aacctattac tcgctctctt gccataactg cccggacgtg gtgcaggcgc
     2281 tgaacctgat gagcgtactg aacccgcgca tcaagcacac tgcaattgac ggcggcacct
     2341 tccagaacga aatcaccgat cgcaacgtga tgggcgttcc ggcagtgttc gtaaacggga
     2401 aagagtttgg tcagggccgc atgacgttga ctgaaatcgt tgccaaaatt gatacgggcg
     2461 cggaaaaacg tgcggcagaa gagctgaaca agcgtgatgc ttatgacgta ttaatcgtcg
     2521 gttccggccc ggcgggtgca gcggcagcaa tttactccgc acgtaaaggc atccgtaccg
     2581 gtctgatggg cgaacgtttt ggtggtcaga tcctcgatac cgttgatatc gaaaactaca
     2641 tttctgtacc gaagactgaa gggcagaagc tggcaggcgc actgaaagtt cacgttgatg
     2701 aatacgacgt tgatgtgatc gacagccaga gcgccagcaa actgatccca gcagcagttg
     2761 aaggtggtct gcatcagatt gaaacagctt ctggcgcggt actgaaagca cgcagcatta
     2821 tcgtggcgac cggtgcaaaa tggcgcaaca tgaacgttcc gggcgaagat cagtatcgca
     2881 ccaaaggcgt gacctactgc ccgcactgcg acggcccgct gtttaaaggt aaacgcgtag
     2941 cggttatcgg cggcggtaac tccggcgtgg aagcggcaat tgacctggcg ggtatcgttg
     3001 agcacgtaac gctgctggaa tttgcgccag aaatgaaagc cgaccaggtt ctgcaggaca
     3061 aactgcgcag cctgaaaaac gtcgacatta ttctgaatgc gcaaaccacg gaagtgaaag
     3121 gcgacggcag caaagtcgtt ggtctggaat atcgagatcg tgtcagcggc gatattcaca
     3181 acatcgaact ggccggtatt ttcgtgcaga ttggtctgct gccgaacacc aactggctcg
     3241 aaggcgcagt cgaacgtaac cgcatgggtg agattatcat tgatgcgaaa tgcgaaacca
     3301 acgtcaaagg cgtattcgca gcgggtgact gtacgacggt tccgtacaag cagatcatca
     3361 tcgccactgg cgaaggtgcc aaagcctctt tgagtgcttt tgactacctg attcgcacca
     3421 aaactgcata agaagaagta agattcacct gcaattgctt agccgccggg gtcaaacctg
     3481 gcggcttttt tatggcatta aaaagccctg ccgggatgtt ccaggcaggg cggcataaat
     3541 acttttatct gttagcgaat gaccagtaca ggcagattgg cgtggcggat cacgctcgag
     3601 gcgttagaac ctaacagatg ggtcgaaatc gatgggttgc gagaaccgat aactacaaca
     3661 tcagccccca gttcttctgc caactcattg acttcatccc gcacgctacc aaaacggaca
     3721 tgttgtttaa tgcgggaagg atcgatggtg aagtggctga ccatcgtttg cagacgttct
     3781 tctgcttcat gttgcagatg ctcttcaaaa cgacgcacat cagcggcaaa acggtgcagg
     3841 ctcaggctgg ctgacccggg tagtacgtga agtagatgaa taactccgtc atcctgggcg
     3901 aggaattcag cgtggcgaac agctttgtcg ctcaattcca tttcaaatac atcaactggc
     3961 ataatgattg tcttatacat aaccctttct ccctgttaat catgaacaaa tcattcgcca
     4021 tgattataat atttatccct gatatttgtc tggttctttt tccttacgaa cggtttctgt
     4081 gattaatata ttctcactga acaccaggaa ttctcccaaa acctgcggta ccgcccgttt
     4141 tcccgctgtg atagctaccc ttaaagactg actctttttt gaactgtctc tggaggttgc
     4201 atgaaagcat tgacttatca cggcccacat cacgttcagg tagaaaatgt tcccgatccg
     4261 ggcattgaac aggctgatga tattattctg cgtattacgg caacggcgat ctgtggctct
     4321 gacctccatc tttatcgagg caaaataccc taggttaaac atggcgatat tttgggtcat
     4381 gaatttatgg gggaagtcgt tgaaaccgga aaggacgtaa aaaatttgca aaaaggcgac
     4441 cgggtggtaa ttccgtttgt cattgcttgt ggcgactgtt ttttctgtcg aatgcagcaa
     4501 tatgccgcct gcgaaaatac caatgcgggt aaaggcgctg cgctcaataa aaaacagata
     4561 ccagctcccg cggcattgtt tggttatagt cacctgtatg gtggcgttcc tggtggacag
     4621 gcggaatatg tccgcgtccc taaagggaat gtggggccgt ttaaagtacc gcctttgctt
     4681 tcagatgata aagccctttt cctttctgat attctgccaa cggcatggca ggcagcaaaa
     4741 aatgcgcaga tccaacaagg ttcaagcgtt gcagtctatg gtgccggtcc agtgggattg
     4801 ttgacaatcg cctgtgcacg gttgctcggt gcggaacaga tttttgttgt tgatcatcat
     4861 ccctaccgct tgcgtttcgc cgctgaccgc tacggcgcga tcccgattaa tttcgatgaa
     4921 gatagcgatc cggcacagtc aattattgaa caaacggcag gtcaccgggg cgtggatgca
     4981 gtaatagacg ccgtcggttt tgaagcgaaa ggcagcacca cggaaacggt gctgactaac
     5041 ctgaaactgg agggcagcag cggtaaagcg ttgcgccagt gtattgcggc ggtcaggcgt
     5101 ggcggcattg ttagcgtacc gggcgtctac gctggattta ttcacggttt cctgtttggc
     5161 gacgcctttg ataaagggtt gacgtttaaa atgggacaga cccacgttca cgcatggctg
     5221 ggtgaattat taccgttaat tgagaaagga ttactgaaac cagaagaaat tgttacccac
     5281 tatatgccgt ttgaagaggc cgcccgggga tatgagattt tcgaaaaacg tgaagaggag
     5341 tgccgtaagg tgattctggt acccggtgca caaagcgcag aggcggcgca gaaggcggtt
     5401 tcaggtctgg tgaatgcgat gccgggggga acaatatgat cgtcaggagt ggttttcgag
     5461 gtaaaggaca gccatgacga taatcgccgc cataatcaga aatcctatca ggatgtaaaa
     5521 tgcttctgcc atggttattc ccacaaacga aacggaataa ttttgcagca aaagtgaaca
     5581 gtgagaacca ggaaaaattg ctgattttgc gtaaagagga tgcgagtgca tcctctgggc
     5641 aaagcgagtt atcgcttgtg cagatgggat taaagcaggt agtcgccagc agcttctggc
     5701 tggtattcga gttccagcac ttcaaggtgg gttgcaacgc cgcccggaag ttcccagtga
     5761 atggaatcgc caacgcgcag ccccagcagt gcggcaccta ccggagccat aacggaaagc
     5821 tgagtattgc tatcggtcat ttttgccgga tacaccagcg tgcgcacacg cacttcgcca
     5881 tcgctaagat tgcggaattt aacccggctg ttcattgtca ccacgtcgtg tggcatctct
     5941 tctggcgaac acatttgggc gcgatccaac tctgcgttta acgcgtcggc gattggcaaa
     6001 ccagcatagg cgggttgctc cagcagaata tcgatgcgtt cggcatccag gtcgttaatg
     6061 atgatagttg gtctggacat ttttactcca tgtcgtcggt actgcgagtg tcgcagataa
     6121 acatacccaa aagaaaaccc tcaccgtcag gcggcgaggg tttaactcac atgatgatac
     6181 tgactgttgc tcactctttg aagtgatttg cgtcacattc agggaattcc tcaatgcagc
     6241 gcattatgta taattcttaa tcgtcacggt ttatggaaga cgaattagcg tgttttgtaa
     6301 atcagatgat taataaccca ctttatcaat cacaaagttt ttgccacagt tgcctgggtg
     6361 tggttgtggc aagaatgagt ttgcagaaag cggagcgttg atggcgtcag ctttgatcga
     6421 gatctgaatt tcagtcaaat acgcagggtt accctggcac gttagcttaa ctgctttcac
     6481 gttctctttt ccccagcttt tggcaaaggc ggcgtcaaag tcacggcggc tcactgtttt
     6541 accgtaatta tccgcaagga attttccggc ttcgctctct ttaatttctt gattcaggcg
     6601 taccatcgta ccgaagtatg cgtccggatc aaaaccaaag caggcaccgt gtttggcata
     6661 ttcgtagcgt tccaggcagg aacgtccgcc agctcctggc atgacttcac ttagtttagc
     6721 ggccgtttcc agtgataacc cggtttccgg cgatgaacac atccggctgg cgtgcgcttc
     6781 tggtagattc gggattgggc gagtagcgca accgaagcgc atccagcggc gttcatcaac
     6841 accacgggca gcaaccgatt taggcaatcc tggccacaga ccatgtacgg tcagaaaatc
     6901 agctttgttg gtcgtttcgg tttgtaggcg acattcatct cgttcgttac gatttcgatc
     6961 gtgttgactc tggcaaaatc cggtttgcca ggagagggcc aggacatagc gatcaaaatc
     7021 gccatactgt tttgcctgca acgctacggc gttggcagaa gagaagggaa gcagagaaac
     7081 cgcgagcaac gcggcgttac gccagaatgc tttcataatg ggtgtggaac tcatacatac
     7141 actgaatact atctattaaa tcataaaaaa gcccgccagg tgggcttacc tggcgggcgt
     7201 gatgatttat tcagcgtttg gcgaacgtat tagttccaca tggcgagaat cggccagcca
     7261 accaacagca acatagagat gtaaatcacc ccgaagattg cgccaagacg ccagtaatct
     7321 tttgatttca catagccaca gccgtaaata atcaccccag gaccggttgc atacggcgtc
     7381 agacagccca tgataccgat agacagcacc agcaggatac acagttgttc cattggtacg
     7441 cccggaatac ctttaccgac ggccagaata accgacagca tggttgcggt gtgcgcagac
     7501 aggctggcaa acaggtagtg tgcaaagtag aacaccagaa ccagtacaat caccgttgcg
     7561 tttggtgaga atccttccag gtgcgtactc atggtaccgg cgaaccagtc aataaagcca
     7621 gaacgagtca ggccgttagc catcacaacc agagttgcca ggttgaccag cgtgttccat
     7681 gcgctgttat agcgggtaat atctttccaa ggcacaacgt gcagggccag cattagcgaa
     7741 actgccagca gaccaactgc agtagcatta atgacttcac tgccaaaaac ccacaaacct
     7801 aagctgagca atacaaggcc aatcagtgtc cactctctgc gtgtcagcgc acccatggtt
     7861 ttcagttcat cacccgccca ggttgccact tcttcactgt gtgtgatttc cggtttgtac
     7921 agcacgtagg aaagccacgg cgcaatgata agcaagataa ccccaaccgg caggaagcag
     7981 aggaaccact gcaaccagct aatctggata ccggcaattt tgctgacgaa ctccagaccc
     8041 agcacgtttg gtgccgcacc ggtgacaaac atggacgaac tcagactggt actaatgacc
     8101 atcatccaca tcaaatagcc gccaatacga cgcgcggacg gatcgttggg gaatgattta
     8161 aacagcggcg gcaggttttt aatgaccgga aaaaccgtac ccccggtacg cgcggtgttg
     8221 gacggtgtaa acggtgccag cagaatgtcg ataatgacaa tcgcataacc caacgtcagc
     8281 gtgcgtttgc ccatgaattt caccaggaaa agggcaatgc gacgacctaa cccggaaact
     8341 tcatacccta atgcaaaaat aaatgcgcca aataccagcc ataccgtggt gctggaaaaa
     8401 ccagccagac cccatttcag cgcctgtttt tgcgcattaa acgctgggtc agctaattct
     8461 ttggcatcaa agagcaggta attactgcca ataacgcaaa tagtaaccgc aataaaactg
     8521 atcgctgttg ccggaattgg ctcgaggatc atgccgacaa tcattgccac aaacacagcg
     8581 aagtaatgcc atgcctgtgg tggcataccg tcggggacag ggataagaaa catgacaccc
     8641 atcaccacca gtggggccaa tagtttccat atattatctt ttgctaaaga catacgggtt
     8701 ctccgaaaat taatatttcc aaatttatca agtgcttaaa taattaaatc tgtgctaaaa
     8761 accaggtaag gatcagcagg tcggcactgc cgcctggact gagatttcgt tcgatacact
     8821 ccctgtcgaa ctgccggaga taatcgagat cggcgggggt tcgaatgccc cctttttgca
     8881 ataatgtttg cgcctcgcgc tgtagccagc gcaggccccc ctcgccaccg cgcgatgcaa
     8941 cgttggtatc gccgttgatc gccatcagta ggagcaaggt atcgagcaat gccagttcag
     9001 gatctaaccc ctgatccagc agagtgaggt aatgcggcaa ggcgtgattg atcaccagtg
     9061 gataacccgc ttcggcttca ccgcgtgcgc cggtaaggcc aagctgctgg tacaaccgtt
     9121 gacctgccgt cagttgtgaa ttattggtac gcagttctcg atcggtcagg ccacggcaga
     9181 aacttgccgc cgtagaacaa acggttgttg gcgttaccgg ttggttgagt tgaagcaaac
     9241 ggccaattgc cgcacatagt agccctaaag aaaaaatgct gcctttatgc gtgtttacgc
     9301 ccgcagtggc gcggaacata tcaccttcgc aagccatacc aattgggcgt aatccgtgga
     9361 gtaccgcttc tggtgctatt tccgcactac aggcaccaaa ttcaatgaaa cggggtagcc
     9421 agccctgaat cgccagcgcg ctgcggtgga aatcttccag cgccatatct ttatgtgcac
     9481 cgcagttaat ccgatccacg aggcctggtt tcggtgacag attgacttca gtcagcatgg
     9541 cgcgccagcc cagcagggcg tactcatcga tcaatgacgt cgcaagcttt gtggttttag
     9601 ttgacgttgc aggcatcgac atcgttcagc agtgcctcca tgcggttgag taaatcggtc
     9661 agttgatggg tttttccacg cgcgcagacg gctgcgcttt gctcgcacaa caggcagcgg
     9721 cgaggcggca gtgaatagtc gcggcgggag agaatttcgc cttcgggcgt caggacatcg
     9781 atatcccata accgcccgag aggatgacta tgttcaagct caatggtggc gagcttgagg
     9841 tcgcgagccg gggcggcaat gctcaacatg ccctccggcc cgctggcgga aaccagtgca
     9901 gcctgctcct gaatttgcca gccctgtttt gcggctaagg cacgcaaggc tgtcacgcca
     9961 tgattaaaaa ttcggcgtgt gacctcgctg tctttaatcg gcccaggcgc aaccacggta
    10021 aaggagacca gtggaacagg atggcgcttg agccagacgt gttgccgtgc ttgcctttca
    10081 tcccggctga cgagcagctc gggaattgac accgcatggt ggctggcgag ttcaggaagc
    10141 aggtgcatgg cttattcctt cacctgatgc acaacatcga tcaccgagcc atcgcggtaa
    10201 cgcacaacgg caacgacgcg gtcggtgaat tcaatcggct gtggttcacc ggtcagcaga
    10261 cgcgcgcgtt cgcgcagcca ctcaatggaa accactttaa tgcccgcttc ctgcagacgt
    10321 tctgccagtt ccggacgtgc cgggttaact gcgataccgt ggtctgtgac cagaatatcg
    10381 acactggagc ctggggtgat gcaggtcagc acgttatcca ccagagtcgg aatacgaccg
    10441 cgtaccagcg gcgcgacgat gatggaaagc gcagaggcaa tcgcggtatc cagtgaccac
    10501 cggaagcacc acgcagtacg ccgtcagagc cggtcagcac gttaacgttg aactgggtgt
    10561 caatttccag cgcgctcagt accaccacgt cgagacgatc aaccgatgcg cctttcgaac
    10621 cccagttagc gtactggttg gcgctgattt cgatgtgatt ggggttacgg gccagcgatt
    10681 gcgcagcatg gctgtcaaag ctctgcacat ccagcagttt gcggatcaga cctttttcgt
    10741 gcagatcaac catcgtcgcg gtaataccgc cgagggcgaa gtcggcgcga atatcgcggc
    10801 tacgcatttt gtcttccagg aaacgggtta ccgccagcga tgcgccgccg gtgccggttt
    10861 gcatggagaa accttctttg aagtagccag agttgacaat cacatccgca gcgctacggg
    10921 caataagcag ttcgcgcggg ttagtggtca tacgggtcgc gccagcgccg atttttgcag
    10981 catcgccaac gcggtcaact ttgacgatca aatcaacctg atcttgctca atgcttgccg
    11041 gattatgcgg ataaggcagc agttcttcgg taagcatcac aacctgtttt gcgttgtcgg
    11101 catcaaccat tgcatagccg agggagccgc agcaggcttt accggtgtaa ccgttggcat
    11161 taccgaattc atcacaggac gggacgccga ggaaagccac gtcgatattc agttcgccgc
    11221 tctgtaccag atgcacacgt ccgccgtgag agtggatttg caccggttcc gcaagcagac
    11281 cacgggaaat ttcttctgcc agtggaccgc gcaggccgga ggtataaatg cgggtaacca
    11341 cgccctggcg aatgtgttct accagtggcg catggcaatc actcagggag ctggacgcca
    11401 gggtcaggtt tttaaagccc atcttagcga tgacgtccat caccatattg acggtcaggt
    11461 caccgccacg gaaagcgtga tggaaggaaa ccgtcatgcc gtcctgtaaa ccagagcgac
    11521 gaatcgcttc ttcctggttg gcgcacagtt tgcgatcgcg cgctttttca gcctggtagg
    11581 tttgctttgg tgagttctgg aaagcggcaa gatcgcattc agcgcgacga ttccaggccg
    11641 ctacccgttc ttgtcgttga gattgttcaa ttttctgcgt cattttgatt gccttattct
    11701 tcgcggatgc cggaaagttc tgcacgggag agcaccagac gggcacgatc gataaccgga
    11761 ccgtccacca tcttgccgtt cagggaaacc acgccgaggc cttcgcgagc ggcggcttca
    11821 gcggcttcta cgacgcggcg ggcgtgatcc acttctttct gggtcggtgc gtagaggttg
    11881 tgcaacagat caatctgacg cgggttgatc agcgatttgc cgtcaaagcc cagctgtttg
    11941 atgtgggcgg cttcttgcag gaatccggct tcgttgttag cgtcggaata gacggtatcg
    12001 aacgcctgaa tacccgcaga gcgcgcggcc tgcaaaatgg aacagcgtgc gaacagcagt
    12061 tcagttcctt ccggggagcg ttctgtacgc aggttgcgca catagtcttc tgcaccgagg
    12121 gcgataccga tcaaacgctc ggaagcgtga gcgatttcca ctgcgcgggt aatccccagc
    12181 ggagattcaa tcgccgccag caggccggtg ctgccgggtt cacgaccaca ggctttttcg
    12241 atacgcagga tctctttttc gatatccaga acatcctgag cggtatcggt tttcggcaga
    12301 cgcacaacgt ccgcaccacc gcgaacgacg gcttccaggt cgttgacacc ccattcggaa
    12361 tccagcgcgt tgacacgcac aatggtttca atatcgcgat acagcggatg ttgcagcgcg
    12421 tggtaaacca tgcggcgggc ggtgtctttt tcacgcaatg ctacggagtc ttcgaggtca
    12481 aacatcaggg catcagccgg gtagatgaag gagttgctga ccatcgcggc attggcacca
    12541 ggcacaaaca acatgctgcg gcgggtgcga gttttacgtt gttgcagcga agcggaaatc
    12601 attggcaatc ctcccatggc agagccggga taccgctggc gcgtgccagc agggcttcca
    12661 gtcgtgcacg taaaatgcag tccagtgcgc ctttgtcatc gacattcagc tgtacgccgc
    12721 gcacgttgta gcgggcgaga acgtccagaa tggtggtgcg aattgcatcg ccaaactgtt
    12781 tctcaacgct gctattgatt tgcaggtcga tatcctgcgt atcgagtggg gcgatgcgta
    12841 tcatcacatc cccagactca agggtgcctg caacggcggg ctggtttatt ttcatttttc
    12901 acctgtttct catgcggggg tcttttgacg agctgccgcg tcctggcggg agtgctcaag
    12961 caggttctgc aaataatgca gcgtgactgc agggaccagc ggcgcgatag ccgtgagatc
    13021 gtttttcgcc agcagttgac gtacccggga agcggatatc ggcatctcct ggtaacgcag
    13081 ccgctcaatt tcaaccagtt cgatgggcgg tgcggagata gtcggcgttt ccagccagta
    13141 gcgcatatcc tggttgtact gggcggtaac gcgacaaaag ggttcagtac cgacaaagcg
    13201 gtgagttaca cccagcgcgg gagcgaggta ctgacggaaa atcttcagat caatttcggt
    13261 gtaacaatgg ttaatgacgc tctgttcttt aatgaagtag caagggaacg tagcgcggga
    13321 gatgatgtat tcggagccgc gatgcacagt caggcgtgga atatcggcgg tgccttttaa
    13381 caccagatcc aaccggtctt cataggggaa gcgtgaagaa tcttctttga ctaaaaacag
    13441 atgcaaccag tcgcactgtg ccgcagcttg ttgaatcaga taacggtgac cattcgtgaa
    13501 gggattggcg ttcatcacaa tgcagccaat cttgttccct ggatgacgaa attttttcag
    13561 cgattcggca tagcgtttca gccgcgtggc gctgttttcc atcagcacca tcacgccggg
    13621 tacgctggtc agcgtggaaa aaccgcactg gcggaacagc gcctcgtatt cggttttggt
    13681 ataaataaac agatgcgtgc tgtgccgctc ataggcgagg tttatcaatt cagtggctaa
    13741 tgtcagcgcc agtccttcac cgcgaacgga ttcactgata gcaacgcatt taataatatt
    13801 tccggca
//
LOCUS       DAZPAC010000105        13793 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZPAC010000105 DAZPAC010000000
VERSION     DAZPAC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13793
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.105"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..146)
                     /locus_tag="ACHUPM_002560"
     CDS             complement(<1..146)
                     /locus_tag="ACHUPM_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFR0870299.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKA"
     gene            complement(343..474)
                     /locus_tag="ACHUPM_002561"
                     /pseudo
     CDS             complement(343..474)
                     /locus_tag="ACHUPM_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            475..1020
                     /locus_tag="ACHUPM_002562"
                     /pseudo
     CDS             475..1020
                     /locus_tag="ACHUPM_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1062..2063)
                     /gene="gap"
                     /locus_tag="ACHUPM_002563"
     CDS             complement(1062..2063)
                     /gene="gap"
                     /locus_tag="ACHUPM_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR0870300.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2252..2782
                     /gene="cybB"
                     /locus_tag="ACHUPM_002564"
     CDS             2252..2782
                     /gene="cybB"
                     /locus_tag="ACHUPM_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR0870301.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3027..3200
                     /locus_tag="ACHUPM_002565"
     CDS             3027..3200
                     /locus_tag="ACHUPM_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870302.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            3226..3294
                     /gene="yncP"
                     /locus_tag="ACHUPM_002566"
     CDS             3226..3294
                     /gene="yncP"
                     /locus_tag="ACHUPM_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR0870303.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(3272..3421)
                     /gene="hokB"
                     /locus_tag="ACHUPM_002567"
     CDS             complement(3272..3421)
                     /gene="hokB"
                     /locus_tag="ACHUPM_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR0870304.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3820..5460
                     /gene="trg"
                     /locus_tag="ACHUPM_002568"
     CDS             3820..5460
                     /gene="trg"
                     /locus_tag="ACHUPM_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR0870305.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5498..6421)
                     /locus_tag="ACHUPM_002569"
     CDS             complement(5498..6421)
                     /locus_tag="ACHUPM_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0870306.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6638..7981
                     /locus_tag="ACHUPM_002570"
     CDS             6638..7981
                     /locus_tag="ACHUPM_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0870307.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8206..9861
                     /gene="opgD"
                     /locus_tag="ACHUPM_002571"
                     /gene_synonym="mdoD"
     CDS             8206..9861
                     /gene="opgD"
                     /locus_tag="ACHUPM_002571"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR0870308.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            10001..10225
                     /locus_tag="ACHUPM_002572"
     CDS             10001..10225
                     /locus_tag="ACHUPM_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR0870309.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            10288..10824
                     /gene="rimL"
                     /locus_tag="ACHUPM_002573"
     CDS             10288..10824
                     /gene="rimL"
                     /locus_tag="ACHUPM_002573"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR0870310.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(10819..11799)
                     /gene="ydcK"
                     /locus_tag="ACHUPM_002574"
     CDS             complement(10819..11799)
                     /gene="ydcK"
                     /locus_tag="ACHUPM_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR0870311.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            11923..12915
                     /gene="tehA"
                     /locus_tag="ACHUPM_002575"
     CDS             11923..12915
                     /gene="tehA"
                     /locus_tag="ACHUPM_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR0870312.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            12912..13505
                     /gene="tehB"
                     /locus_tag="ACHUPM_002576"
     CDS             12912..13505
                     /gene="tehB"
                     /locus_tag="ACHUPM_002576"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR0870313.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3540 a         3197 c         3483 g         3573 t
ORIGIN      
        1 gccttacggg catcctcggc ctgaccatcg ggtatacggg aaatgacagc ctctgtagca
       61 gggtttacca catcaatcca tgcgtctcca cgccaggtaa caaactgtcc atcgatatac
      121 ataggatgtt gaacgggtac tgacatgggc gactcctgtg atttatatgt ttgttttcgg
      181 tgaatatatt gttaattaac atcaatcatc agagagacgg catcgggaaa ggacaattct
      241 gtgaagggct tcataaaaac aagtgtaaaa ggaataaaag gcagaaataa aagtcaggag
      301 tcatgcccgt ttgcaaacaa gtggttcaca aacgggcaat ttttaacgta acgagcgact
      361 ttccatcgcc tcgatgagca ccgcatcatg tttcagcgtt tgccatgcat ggatgacttc
      421 tgccggaaaa tcaacgtgaa cgataaaatc tcgcccgcgg ggaccgtagc cattgatcag
      481 ttcgtcaatc ggctgggtga agcgatgcag gcggttaaat ttggtaaccc cgctgaacgc
      541 aacgacattg cgatggggcc gttgattaac gccgcggcgc tggaaagggt tgagcaaaaa
      601 gtggcgcgcg cagtagaaga aggggcgaga gtggcgttgg gtggcaaagc ggtagagggg
      661 aaaggatatt attatccgcc gacattgctg ctggatgttc gccaggatat gtcgattatg
      721 catgaggaaa cctttggccc ggttctgccg gtggtcgcat ttgacacgct ggaagaggct
      781 atctcaatgg ctaatgacag tgattacggc ctgacctcat caatctatac ccaaaatctg
      841 aacgtcgcga tgaaagccat taaagggctg aagtttggtg aaacttacat caaccgtgaa
      901 aacttcgaag ctatgcaagg cttccacgcc ggatggcgta aatccggtat tggcggcgca
      961 gatggtaaac atggcctgca tgaatatctg cagacccagg tggtttattt acagtcgtaa
     1021 tgagtaaaag aggcggaggt tttttcctcc gcctgtgcgc gtcagagttt agcgaatttt
     1081 tcgagggtgc gaataagctg cgtgacgaag ccatattcgt tatcgtacca ggcgaccgtt
     1141 ttcaccagtt gtaaatcgcc cacggcggta atttccgttt gcgtggcatc aaacaccgaa
     1201 ccgaaatggc tgccaatgat atcggaagag actatttctt catcggtata accaaatgac
     1261 tcgttattgg tggttgcttg tttaagtgcg ttattcacct cttcggcagt cacttttttt
     1321 ccgagaatcg ataccagttc agtgaccgaa cctgttttca ccggcacgcg ttgcgcatga
     1381 cctttcagtt tgccgctcag ttccgggatc accagaccaa tggcttttgc cgcccccgta
     1441 gtgtggggaa tgatattttc tgccgctgcg cgtgaagcac gtaaatcttt accacgtggg
     1501 ccatccacca gtgactgggt gccagtatag gcatgaatgg tcgtcatcgt gccgacttct
     1561 atcccgaaac tgtcatgcaa ggctttggcc atcggcgcaa gacagttagt ggtgcatgac
     1621 gccacggaaa caatggtgtc gttgccatcc agagtgtcgt cattgacgtt ataaacgata
     1681 gttttcattt caccggcagg ggcggaaatc aacaccttct tcgcaccagc atcaagatgt
     1741 gcctgcgatt tctcggcgga ggtataaaag ccagtacatt cgacaatgat ttctgcacct
     1801 ttcgctttcc acggaatatt tttagcctct ttttcggcgt aaaccgcgat acttttccca
     1861 tcaacgataa gtgaatcttc cgtaaaatca acgctccagg ggaatggtcc gtagtttgaa
     1921 tcatgtttca gcaggtaggc gagaattttt ggggaagtga gatcattaat agcgacaacg
     1981 tctatgttgc ttttgacttc aagtaatcga cgcaacacca gtcgaccgat acgaccaaaa
     2041 ccgttaatac caactttact catggttttc tcctgtcagg aacattcgga tgaaaattga
     2101 tcctttccag gcttagacca ggattgtggg atgggcaatc tccattctta cagtgaaacg
     2161 taacgtactg aaaacgggtg aacaatattt aatgaaattt tgagaaaagc ccgttatgtt
     2221 aacggaaaat tatgttaaag caggaaatgt tatggaaaat aaatactcaa ggttacaaat
     2281 cagcattcac tggctggtct ttttgctggt tatcgcagcg tattgcgcaa tggagtttcg
     2341 tggtttcttc ccacgtagcg atcggccact catcaacatg attcatgttt cctgcggcat
     2401 ctcaattctc gtgctgatgg tcgttcgtct tttgttaagg ctgaaatacc caaccccgcc
     2461 gattatacct aagccaaaac cgatgatgac gggactggcg catttgggac atttggtgat
     2521 gtatctcctg tttattgcgc tgccagtgat tggtttggtg atgatgtata accggggcaa
     2581 cccgtggttt gcgtttggtt tgacgatgcc ttacgcttca gaggccaatt tcgaacgggt
     2641 tgatagctta aagtcgtggc atgagacgct ggcgaatctg ggatattttg tcatcgggtt
     2701 gcacgctgcg gctgcactgg cacaccacta tttctggaag gacaacacac ttctacgcat
     2761 gatgccgcgt aaacgttcct gaaggatatt tcaagaaaat gcctgtacta aaaccgaccc
     2821 gtggtacagg cgaagaatac gggtctacat cggaagcgcc tattatattt atttgtatga
     2881 caaataaaac cataatcctt gcccatacgt ccatctggct tatttttaat caattcaccc
     2941 gatctttgat ctcatcaacg gtatcaaaat aaaccagcgt ataacgttca aatcgaacat
     3001 aagattcgat aaaccatgga ggttatatga aaaaactggc acttattttg tttatgggaa
     3061 cgcttgtttc cttttatgcc gatgccgggc gcaaaccctg ttctggttcg aaagggggga
     3121 tctcacactg tacggcaggc ggcaaatttg tctgtaatga tggttctatt agtgcatcga
     3181 aaaaaacatg cactaactga agtgtaaaag gggtgccatg agaaaatgaa tctgctagtc
     3241 agatgcgcgg ggaaaatccc cgcgcttgcc cttacctgga cgtgcaggcc atgagcgcag
     3301 caacctcctt atcaccgtcc cggaaccgca gttcgtagag cgtttgtcgg gtcaggagtg
     3361 tgaatgtcag aatcgtaatg cagataatga gcagacacac taccagaggg ttgtgcttca
     3421 tagcctttct ccttgccgga tggcgggtaa gaggctaaga tctgaattgc taggttcatt
     3481 cgttggcctc ggttgataga aatatcggtc ggggccttcg tctttctgat tcccggttag
     3541 cctgaaaaca gaaagtctca ggcacccgca ggcatcctat gaggtttcct tagggacgaa
     3601 aataatcact tcacgaaatt gcgtgctgtt ttccagaatt tttcgtcatt cgggttagcc
     3661 agtttagcca ttcgttactc tcttcattcc aatagcatta attttctctg caataattgt
     3721 tgtaaaaatg tgacgcaaag aggtttttgg tcataagtaa ttaccgtcaa gtgccgatga
     3781 ctttctatca ggagtaaacc tggacgagag acaacggtaa tgaatacaac tccctcacag
     3841 cgattaggtt ttttgcatca catcaggctg gttccgttat ttgcctgcat tcttggcggt
     3901 atcttagttc tattcgcatt aagttctgct ctggctggct atttcctctg gcaggccgat
     3961 cgcgatcagc gtgatgttac tgcggagatt gagattcgga ccgggttagc gaacagttca
     4021 gattttttgc gttcagcccg gatcaatatg attcaggccg gggctgcgag ttgtattgcg
     4081 gaaatggaag caatgaagcg aaatattgcg caagccgaat cggagattaa acagtcgcag
     4141 caagggtatc gtgcttatca gaatcggccg gtgaaaacac ctgctgatga agccctcgac
     4201 actgaattaa atcaacgctt tcaggcttat atcacgggta tgcaacctat gttgaaatat
     4261 gccaaaaatg gcatgtttga agcgattatc aatcatgaaa gtgagcagat ccgaccgctg
     4321 gataatgctt ataccgatat tttgaacaaa gccgttaaga tacgtagcac cagagccaac
     4381 caactggcgg aactggccca tcagcgcacc cgcttgggtg ggatgttcat gattggcgcg
     4441 tttgtgcttg ccctggtcat gacgctgata acatttatgg tgctacgtcg gatcgtcatt
     4501 cgtccactgc aacatgccgc acaacggatt gaaaaaatcg ctagtggcga tctgacgatg
     4561 aaggatgaac cggcgggtcg taatgaaatc ggtcgcttaa atcgtcattt acagcaaatg
     4621 cagcattcac tggggatgac agtagggact gttcgacagg gtgcggaaga gatttatcgt
     4681 ggcaccagcg aaatttcagc tggcaatgcg gacctgtcat ctcgcaccga agaacaagcg
     4741 gcggctatcg aacaaactgc cgccagcatg gagcaactca ctgcgacggt gaaacagaat
     4801 gcggataacg cgcatcatgc cagcaaactg gcgcaagagg cttctattaa agccagcgat
     4861 ggcgggcaga cggtttccgg tgtagtaaaa acgatgggcg ctatctccac gagttcgaag
     4921 aaaatttctg agatcaccgc cgtcatcaac agtattgctt tccagacgaa tattctggca
     4981 ctgaatgctg ccgttgaagc cgcgcgagcg ggtgagcaag ggcgtggatt tgccgttgtc
     5041 gccagcgaag tacggacact cgcaagtcgc agcgctcagg cggcgaaaga gattgaaggc
     5101 ttgatcagtg aatcagtcag gttaattgac ctggggtcgg atgaggtggc aacggccggg
     5161 aaaaccatga gcactattgt tgatgccgtc gcgagtgtca cacatatcat gcaggaaatc
     5221 gccgccgcct cggatgaaca aagtagaggc ataacgcagg ttagccaggc gatttctgaa
     5281 atggataagg tgacgcaaca gaatgcttct ctggtagaag aggcctcagc ggcggcggtg
     5341 tcccttgaag aacaggcggc acgattaact gaggcggtgg atgtattccg tctgcacaaa
     5401 cattctgtgt cggcagaacc tcgcggagcg ggtgaaccag ttagtttcgc tacggtgtga
     5461 aaatgttcaa ggagggatcg acagatccct tcacctttca gaacggcatt gattttcgaa
     5521 tagcgttaat catcaactgg caaccagaag agaacgtcgc atctacgcgg gtcagtattc
     5581 caatcggttc gcctgcacca tgtcccggaa caggcagggc caccagcgtg gcatgacgca
     5641 ggtcgtcttt tacagcgcca gaagggacaa accacacgta atcgtattca accgtaagtt
     5701 gacgagatag cgaagcagac agcgtttcga tacaacccga aggaatttta cagccctggc
     5761 tctgcactaa tgcatctgaa tgctggcgtg gcgcagtgcc ttctggtgat acaacgaccg
     5821 gccattccag cacccggctt agcgttacgt tctcctgaag tagcgggtga ttagggcgga
     5881 caaccagctt cagcgattca agaaacagca gttcgtaatt aagcccggtc atcagttcag
     5941 gatctgacat ccgaccaatg ccgatatcga tttccccggt ttttaaaccc gccagaatca
     6001 tagggttact cattgtcgca acttgcaagg tcgtctcttt ttgttgctga tgaaactgac
     6061 ctataaccga aggtaatatc cccagtgccg cagtaggtag tgcaccaacc ctgacgacat
     6121 cattattaag accttcttta cgatgaagcg actgtccggc agtgttgatg gcgtcaagaa
     6181 ctctgactgc atgcgttaaa aattgttcgc cgggtaaggt aagttgcgcc ccctgacgac
     6241 cacgctcaaa caagcgagcg cccgtcagct gctccagttc attcaatgtc ttagagagcg
     6301 caggttgact caaattaagg gtttcagccg cgcgccccaa agttccttgt tgtgcgacag
     6361 ctacgaatgt atgaaggtgg cgcaaacgga tgcgctgact aaacagacta tttttttcca
     6421 taagcgatgt taaaaacgaa gcggtgtcgc tgacaagtga agttgtttga ttatgataac
     6481 ttgattgcaa aatattatta acaattaaag caattatgtt acagcaaaat ggataatatt
     6541 gatgttttcg cggcgagatc acagtttgta aattcttccc gccagagtga atgcggttac
     6601 ctacactcca gattactgac cactggaggc agacactatg gcgaacagca tcacggcgga
     6661 tgagattcgg gaacagtttt cgcaggcaat gtcagccatg taccagcaag aagttccgca
     6721 atatggcacg ctgctggaac tggtagctga tgtgaatctg gctgtgctgg aaaacaatcc
     6781 tcaactgcac gaaaaaatgg taaatgcaga cgagctggcg cgactgaatg ttgaacgtca
     6841 tggggcgatt cgcgttggga ctgcacaaga gcttgctact cttcggcgga tgtttgccat
     6901 tatggggatg tacccggtga gctattacga tctctcgcag gcaggggtgc cggtacattc
     6961 gacagcattt cggcccattg atgatgcttc tctggcgcgt aatcccttcc gcgtttttac
     7021 ctccttactc cgccttgagc ttatcgagaa cgaaattttg cgccagaaag cggcggagat
     7081 tctgcgtcag cgcgatatct tcaccccacg ttgtcgacaa ctgttagagg aatatgagca
     7141 gcagggcggt tttaacgaaa cacaggcaca ggagtttgtg caggaagccc tggaaacgtt
     7201 tcgctggcac cagtcagcaa cggtagatga agaaacctat cgcgccttgc acaacgaaca
     7261 tcggttgatt gctgatgtgg tctgttttcc tggatgccat atcaaccacc tgacgccacg
     7321 tacgctggat attgaccggg tgcagtcgat gatgcctgaa tgcggaattg aacccaaaat
     7381 tctcatcgag gggccgccgc gccgcgaggt accgatttta ctacgccaga ccagctttaa
     7441 agcactggaa gagacggtgt tgtttgcggg gcagaaacag ggcacgcata ccgcgcgctt
     7501 tggtgaaatt gagcagcgtg gcgtggcatt aacgccgaaa gggcgacaac tgtatgatga
     7561 tcttctgcgt aacgctggaa ccgggcagga taatctcact caccaaatgc atttacagga
     7621 aaccttccgc acttttcctg acagtgagtt tttaatgcgt cagcaagggt tggcatggtt
     7681 ccggtaccgt ctgacgcctt cgggtgaggc gcatcgtcag gcgattcatc ctggagacga
     7741 tccacagccc ttaattgaac gtggttgggt agtggcgcaa cccatcacct atgaagattt
     7801 cttgcccgtt agcgcggcgg ggatcttcca gtcaaatctg ggtaatgaaa cgcagacacg
     7861 cagtcacggt aatgccagtc gcgaagcatt tgagcaggcg ttgggttgtc cggttttgga
     7921 tgagttccag ctttaccagg aagcggaaga acgcagtaaa cgtcgctgtg gtttgcttta
     7981 aattctgacc atccgccttt gcaaaaattt gcctgatttt acaaacgaat caggctcatc
     8041 ccatcgacat aaaaaaaatg ccgatttatg catattctct cagttcaaca attggattat
     8101 taataaatat tgtctagagt gagcggtcat aaataagcac tttcttgccg ctgaaaacga
     8161 ccagcgcggg accattcaca acaccagaag gactcacttt caggtatgga tcgtagacga
     8221 tttattaaag gttcaatggc tatggccgcc gtgtgcggta ccagcggcat tgcttctctt
     8281 ttttctcagg cggcattcgc ggcagattct gatattgccg acgggcaaac ccagcgtttt
     8341 gacttctcca ttctacagtc aatggcgcac gacttagcgc aaacagcgtg gcgtggtgcg
     8401 cctcgtccgt tacctgacac gctggcgaca atgacgccgc aggcttataa cagtattcaa
     8461 tacgacgccg aaaaatcgct ctggcataac gttgagaacc gtcaactgga cgctcagttc
     8521 ttccatatgg gaatgggatt ccgtcgccgc gttcgtatgt tttctgtaga tccagcaaca
     8581 catctggcgc gtgaaattca ctttcgcccg gagttgttca aatacaacga tgcaggtgtt
     8641 gataccaaac aattagaagg gcaaagcgat ctcggctttg ccggttttcg cgtgtttaaa
     8701 gcccccgaac tggcgcgccg tgatgtagta tcatttctcg gcgcgagtta tttccgcgcc
     8761 gttgatgata catatcaata cggtttgtcg gcccgcggcc tggcgatcga cacttacacc
     8821 gacagtaaag aagagttccc cgactttacc gccttctggt ttgatacggt aaaaccgggg
     8881 gcaactacct ttaccgttta tgcgttgctc gatagcgcca gtattactgg tgcctataag
     8941 ttcactatcc attgtgagaa aagtcaggtg attatggatg tggaaaatca cctgtatgcg
     9001 cgcaaagaca ttaaacagct gggcattgcg ccgatgacca gtatgttcag ctgcggtact
     9061 aatgaacgtc ggatgtgcga caccattcat ccgcaaattc atgactctga tcgtctgtcc
     9121 atgtggcggg gcaacggcga gtggatttgc cgtccgctga acaatccgca aaaattgcag
     9181 ttcaatgctt acaccgacaa caacccgaaa gggtttggtt tattgcaact ggatcgtgac
     9241 ttctcccatt atcaggacat tatgggctgg tataacaaac gcccaagttt gtgggtggaa
     9301 ccgcgtaaca agtggggtaa gggcaccatc ggcctgatgg aaatcccaac aacgggcgaa
     9361 acgctggata acattgtctg cttctggcag ccagaaaaag ctgtaaaagc aggtgatgag
     9421 tttgcattcc agtatcgtct gtactggagt gcgcaaccgc ctgttcattg cccattagcg
     9481 cgcgttatgg cgacgcgtac cggcatgggc ggtttcccgg aaggttgggc gccaggtgaa
     9541 cactatcccg aaaaatgggc gcgtcgtttt gccgtcgatt tcgttggtgg tgatctgaaa
     9601 gctgccgcgc caaaaggcat tgagccggtg attacgcttt ccagtgggga agcgaagcaa
     9661 atcgaaattc tctatattga acccatcgat ggttatcgta ttcagtttga ctggtatccg
     9721 acttcggact ctactgatcc ggtcgatatg cggatgtatc tacgttgtca gggggacgct
     9781 atcagtgaaa catggctgta tcagtatttc ccgccagcgc cggataaacg tcagtatgtt
     9841 gacgaccgcg tgatgagtta atcgtttttt cttcggcacc ttcttcggga ggtgccgtct
     9901 ggttaaacac gatcccgctc gcatttttcc ctaagttaaa tgagtaatct gatggtgtgt
     9961 atttcagata caccttgtca gccactaaca gggagtgcgt atgtttccag aataccgaga
    10021 tttaatatcc cgtctgaaaa acgaaaatcc tcgctttatg tccttgttcg ataaacacaa
    10081 taaacttgat catgaaattg ccagaaagga aggttccgac ggtcgagggt acaatgcgga
    10141 agtggtccgc atgaaaaaac aaaagctaca gttaaaagat gagatgctca aaatcctgca
    10201 gcaggagagc gtcaaagagg tgtaaacttt cctaagccgc cagccaggcg gcttttttaa
    10261 caacagcatg gattgactgg agataagatg actgaaacga taaaagtaag cgaatcactt
    10321 gaattacatg ctgttgcgga aagtcacgtc acacctcttt atcagttaat ctgtaaaaat
    10381 aaaacctggt tacagcagtc gctaaactgg ccgcagttcg ttcagagtga agaggacacg
    10441 cgaaaaacgg tgcagggtaa tgtgatgttg catcaacgcg gctatgccaa aatgttcatg
    10501 attttcaaag aagatgaact tatcggcgtt atctcgttta atcgtattga accactgaat
    10561 aaaactgctg aaatcggcta ctggctggac gaatctcatc aggggcaggg gatcatttct
    10621 caggcgctgc aggcattgat tcatcattac gctcagtctg gtgaacttag acgcttcgtg
    10681 atcaaatgtc gggtggacaa tccgcaaagc aaccaggtcg ctttgcgcaa tggttttatc
    10741 cttgaaggtt gcctgaaaca ggctgagttc ctgaatgatg cctatgatga tgtgaactta
    10801 tacgcgcgta ttatcgattc ataataaccc tgccagcggc gttcgcgtaa tgcgctcctc
    10861 gccgttaatg acttttggac cacggacatg aaccgtgtcg ccatcaaagg cttcaacgac
    10921 ggcgtggtcg gttaactcca catggttttc aattatcacc gcacctgaaa tacggctctc
    10981 accttgtatt acaacgtgct cgtcgagcag aatcggtcca ccacgtacca cggcatttcc
    11041 gccaatcaag acatgatgtt ttaacacaca attaccttcc acaatggcat attccgccac
    11101 ctgcgaactg taatgaatcg tcggaatggc atcttcttct atgccagctt tcacctgcgc
    11161 gtgaccgtag acttttgcgc aatcgcatag ccagacgttg ttctcttcat taccttcaac
    11221 actggcaaaa tcaaaaactt cggcgcgatg ttcaataaaa gcataccgga cgacggcatc
    11281 gccataaatt tgtgcctggt ggacaatacg cgaggcgcta acccttgcgc gatcataaat
    11341 ttggagcagg agttgatggt cgggcgttaa gccttgtgcg gcgacgatca tagaatgttg
    11401 atcaattaag gcgtgaccaa atattctaca ttgcccgtaa actaatgagt cacgaatggt
    11461 gacactgtca cttatatatg caccctggct gatttcacta ttatcgatcc agacgttatc
    11521 cgttgcgtaa acctctcccc acagcacgct ggtgccggtg atacgcgtat taccagaaat
    11581 caccgttccg ccgaacgcaa tagcattctg gtcgtaaatc cagcaatttc cttcctgtgc
    11641 taacaccgtt tcgcgatcga tccagccgcc agcggttcct gctatcacat cgttaaaatc
    11701 gctaatggcg ataatctgcc gtaataacac acttttttta gtgccatctt cctgataact
    11761 aaaggctcgc tgttcttcac ttagacgata tttgcgcata gcgttttctc acaggtgact
    11821 tactataacc gtagcaaatt ctgcggctct ggctatgctc gagaaaattc cataaaatgc
    11881 atttcaaata tactttataa attaaacaaa atgagtaaga agatgcagag cgataaagtg
    11941 ctcaatttgc cggcaggcta ctttggtatt gtgttgggga cgatagggat gggatttgcc
    12001 tggcgctatg ccagccaggt ttggcaggtc agccactggt taggggatgg gctggtgatt
    12061 ctggcgatga tcatctgggg attattaact agcgcattta ttacccgact catacgcttt
    12121 ccgcatagcg tgctggcgga agttcgccat ccagtgctga gcagttttgt gagtttgttt
    12181 ccggcaacga cgatgctggt ggcgattggt tttgttccgt ggtttcgccc actggcggtg
    12241 tgcctgttca gttttggtgt cgtggttcag ttggcttatg ccgcctggca aactgcggga
    12301 ttatggcgcg gatctcaccc tgaagaagct accacgcctg gactgtatct gccgacagtt
    12361 gccaacaact ttatcagcgc aatggcctgt ggtgcgttgg gctacaccga cgccggtctg
    12421 gtgtttttag gcgcaggcgt tttctcatgg ctaagtcttg aaccggtgat cttgcagcgt
    12481 ctgcgcagtt cgggagaatt acccacggca ctgcggacat cactcggcat tcagctcgct
    12541 cctgcgctgg tggcctgtag tgcctggttg agcgtcaacg gcggcgaggg tgatacgctg
    12601 gcgaaaatgc tttttggtta tggactgctg caactgctgt ttatgctacg tctgatgcca
    12661 tggtatctct cccagccatt taatgcttca ttctggagtt tctcgttcgg cgtatctgca
    12721 ctggcaacca ccggtttgca tctggggagt ggcagcgaaa atggattttt ccatacgctg
    12781 gcggtaccgc tgtttatctt taccaatttt attattgcaa tactgctcat ccgtactttt
    12841 gcgcttctga tgcagggaaa attgttagtc agaaccgagc gcgccgtttt aatgaaagca
    12901 gaggacaaag aatgatcatt cgtgacgaaa actattttac tgataaatat gaattaaccc
    12961 gcacacactc tgaagtactg gaagcggtga aagtggttaa accgggtaaa acgctggatc
    13021 tgggctgtgg caatggtcgt aacagtcttt acctggcagc caatggttat gatgttgacg
    13081 catgggataa aaatgccatg agtatcgcca atgtagagcg cattaaatcc attgaaaatc
    13141 tggataattt acacacccga gtcgttgatc tgaataacct cacatttgat agacagtacg
    13201 attttattct ttcgactgtg gtactgatgt tccttgaggc taaaaccatc ccagggctga
    13261 ttgccaatat gcaacgttgc actaaacctg gtggttacaa cctgattgtg gcggcgatgg
    13321 ataccgctga ttatccatgt accgtcggct tcccgtttgc cttcaaagag ggagaattac
    13381 gtcgatatta cgaaggctgg gagagggtga aatacaatga agacgtcggc gagctgcacc
    13441 gcaccgacgc caacggtaat cgtattaaac tgcgtttcgc cacgatgctg gcacgtaaaa
    13501 aatgacccgc taagaataag gcgcgtgaaa attccccacg ctgagatgat ttactgttct
    13561 tcttttcggt aagcatattt tttatcgaag ggatgtgaaa ttaatcacag tagtcgaagt
    13621 tttttagcag cttaacttac tgaaatttaa ctactgatga ttgacttagc ccctttttcg
    13681 gcattgacta tgtcgtctga aaaggggctg aaaaatttat tttcaccaac actttttttg
    13741 ccacaacacg aagcggcgct ttttgctata acttaaaaag taatataatc atc
//
LOCUS       DAZPAC010000106        13766 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZPAC010000106 DAZPAC010000000
VERSION     DAZPAC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13766
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.106"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            242..1495
                     /gene="kch"
                     /locus_tag="ACHUPM_002577"
     CDS             242..1495
                     /gene="kch"
                     /locus_tag="ACHUPM_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR0870314.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1550..1723)
                     /locus_tag="ACHUPM_002578"
     CDS             complement(1550..1723)
                     /locus_tag="ACHUPM_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR0870315.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1866..3326
                     /gene="cls"
                     /locus_tag="ACHUPM_002579"
     CDS             1866..3326
                     /gene="cls"
                     /locus_tag="ACHUPM_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR0870316.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3361..3690
                     /locus_tag="ACHUPM_002580"
     CDS             3361..3690
                     /locus_tag="ACHUPM_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR0870317.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="ACHUPM_002581"
     CDS             complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="ACHUPM_002581"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR0870318.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="ACHUPM_002582"
     CDS             complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="ACHUPM_002582"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR0870319.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="ACHUPM_002583"
     CDS             complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="ACHUPM_002583"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR0870320.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="ACHUPM_002584"
     CDS             complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="ACHUPM_002584"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR0870321.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="ACHUPM_002585"
     CDS             complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="ACHUPM_002585"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR0870322.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            9613..9909
                     /locus_tag="ACHUPM_002586"
     CDS             9613..9909
                     /locus_tag="ACHUPM_002586"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870323.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="ACHUPM_002587"
     CDS             complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="ACHUPM_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR0870324.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11192..>13766
                     /gene="adhE"
                     /locus_tag="ACHUPM_002588"
                     /gene_synonym="adhC"
     CDS             11192..>13766
                     /gene="adhE"
                     /locus_tag="ACHUPM_002588"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR0870325.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     IL"
BASE COUNT         3611 a         3407 c         3197 g         3551 t
ORIGIN      
        1 cttatagccg gtcagtgcat tcactaccac aagcagataa taactttatc tgtatttctt
       61 tatatgatgt gagcatattc aggaaaaatg acctttcctg aggtacagtt gctcgctcaa
      121 atcctttgat tactgcggat aaagtcatct actattgttt aacacattca cgcgctgcat
      181 gtatgttcag aaaatgtgca cgctaagata attaaacaaa attgtggtta cagaggtatc
      241 agtgagtcac tgggctacat tcaaacaaac agcaacaaat ttatgggtga cccttcggca
      301 cgacattctc gcgctggccg tctttttaaa tggattgctt atttttaaaa caatctatgg
      361 tatgtcggtc aatttgcttg atattttcca tatcaaagca ttttcagagt tggatctctc
      421 cttgctggca aacgccccac tatttatgct cggcgtcttt cttgtcctga actccattgg
      481 cttactgttc cgggcaaagc tcgcatgggc aatcagtatc attttgttgt tgatagcgct
      541 aatttacacc ctgcattttt atccctggct gaaatttagt attggatttt gcatttttac
      601 gctggtgttt ttgctgatac tgcgcaaaga cttctcccac agtagcgccg cagccgggac
      661 aatttttgca tttattagtt tcacgacatt actgttttac tcaacctacg gtgcgcttta
      721 tttaagcgaa ggttttaatc cgcgaataga aagtttgatg accgcgttct atttttcgat
      781 agaaaccatg tcaaccgtcg gctatggcga tattgtccct gtttctgaat cagcacgatt
      841 gttcactatt tcggtcatta tttccggcat taccgttttt gccacatcta tgacctcaat
      901 ttttggtccg cttatccgcg ggggattcaa caaacttgta aaaggaaaca atcatacaat
      961 gcatcgtaaa gatcatttta ttgtttgcgg acattcgatt ctcgccatca atacgatcct
     1021 gcaactgaat caacgcggac aaaacgtaac ggttatcagc aacttgcctg aagatgatat
     1081 caagcaactt gagcaacgct taggcgataa cgctgatgtt atccccggtg acagtaatga
     1141 cagttcagta ttaaagaaag cgggaatcga tcgttgccgg gccattctgg cgctgagtga
     1201 taacgatgca gataacgcgt ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa
     1261 aacagttctc gccgtcagtg atagcaaaaa cctgaataag attaagatgg tacatccgga
     1321 tatcattctc tcaccgcaac tgtttggcag cgaaattctg gcgcgagttt taaatggtga
     1381 agagattaat aatgatatgc tcgtttcaat gttgttgaac tccggtcatg gcattttcag
     1441 cgataacgat gaacaagaaa cgaaagctga cagtaaagaa tcagtgcaaa aatagagttt
     1501 aaagacgaaa gagtaaactt attgggcacc gcaatggtgc ccagtatatt tagatattgt
     1561 agatcgcaaa caataacgag ttacgctgtt tgtttagaat gcacttcctg atgctgtgga
     1621 tacgcatatt tcggcgcgat tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac
     1681 gctgcatgcg ccagcgcccc acttccgttc tactccgctt catgttaact actctatgca
     1741 ataacagaat ggtcattata cccccacccc cgacgctgac cagtgctttt cccgcgtttt
     1801 tatttgccag atgaatccat atgcgtaaac tcataacaat gcgctttcaa aaggatttct
     1861 aatctatgac aaccgtttat acgttggtga gttggttggc cattctggga tactggttgc
     1921 tcattgcagg cgtaacttta cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg
     1981 cctggctgtt gattatttac attctgccgt tagtcggaat tattgcctat cttgccgttg
     2041 gcgagctcca tttaggcaaa cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg
     2101 caaaatggct taacgacctt aaagcctgta agcatatctt cgccgaagaa aatagcagtg
     2161 tcgctgcgcc attattcaag ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga
     2221 atcagctaca actgatgacc gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc
     2281 agctcgcgcg ccataatatt gagatggtgt tttatatctg gcagcccggc ggcatggcgg
     2341 accaggtggc tgaatcatta atggcggctg cacggcgtgg tattcattgc cgattgatgc
     2401 tcgactccgc cgggagtgtg gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg
     2461 ccggtattga agtggtcgaa gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta
     2521 tggacctgcg ccaacatcgc aagatgatca tgatcgataa ttacatcgcg tacaccggca
     2581 gcatgaatat ggtcgatcct cgctacttca aacaagatgc gggcgtaggg caatggattg
     2641 atctgatggc gcgtatggaa ggccccatcg ccaccgcgat ggggattatt tattcctgcg
     2701 actgggagat tgaaaccgga aaacgtattc tgccgccacc accagatgtc aatattatgc
     2761 cgtttgaaca ggccagcggt cacaccattc acacaattgc ttctggcccc ggctttccgg
     2821 aagatctcat tcaccaggca ttattgactg cggcttattc ggcgcgtgaa tatttgatca
     2881 tgacaacgcc ctactttgtg ccaagcgatg atttacttca tgcgatttgc acggcggcgc
     2941 agcgcggggt ggatgtcagt attatccttc cgcgaaaaaa tgactcgatg ctggtcggct
     3001 gggccagtcg cgcattcttt acggaactgc ttgctgctgg ggttaaaatt tatcagtttg
     3061 aaggcgggtt actgcatacc aagagcgtgc tggtcgatgg cgaactaagt ctggttggca
     3121 ccgttaacct tgatatgcgt agtctgtggc tgaatttcga gattaccctg gcaatcgacg
     3181 ataaaggttt tggtgcagac cttgccgccg ttcaggacga ttatatttcg cgttcacgtc
     3241 tgctcgatgc ccgtttatgg ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt
     3301 tttacttctt cagcccgttg ctgtaaaacg tcgccaacag aggttaaaca ggtagtgact
     3361 atggatatgg atctaaacaa tcgcctgact gaagatgaaa cgcttgagca ggcctacgat
     3421 atttttctcg aactggctgc cgacaatctc gacccagctg atgttctgct gttcaatctt
     3481 cagtttgaag agcgcggcgg cgcggagtta ttcgatccgg cggaagactg gcaggaacat
     3541 gttgatttcg atttgaaccc ggactttttt gccgaagtgg tgattggtct ggcggacagt
     3601 gaggatggtg aaatcaacga tattttcgcc cgcattctgt tatgccgcga aaaagatcac
     3661 aaactttgcc atatcatctg gcgggaataa ataatcgctt tcggcgtgaa aaagggctga
     3721 caactgtcag cccttattgt gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc
     3781 tgaaactccc ttccagcaca ggacgtgttt tggcgcactc cggaccggca atcgggcaac
     3841 aggtacggaa aacacaaccg gaaggcgggt tgatcggcga cggtaattcc ccttccagta
     3901 actggatggt tttgttcttc tccagatccg gatcaggtat ggggactgcc gacatcaatg
     3961 ccctggtgta aggatgtagt ggattgtggt agacctcatc ataggtcccc agttctaccg
     4021 catggccgag atacatcacc aacacacgat cggaaatgtg ttttaccacg gccaggtcat
     4081 gagcgataaa aattaatgac aatcccatct cacgttgcag ctgctggagc aggttgacca
     4141 cctgcgcctg aattgacacg tccagcgccg aaatcggctc atcgcagata atcagcttcg
     4201 gttcaagaat aagagcacga gcaatcccga tacgctggca ctgcccaccg gagaactcat
     4261 gcggatagcg gttaatcagg ttaggcaata acccgacttt cagcatcatc gccttcacgc
     4321 gctcgcgaac ttcctggcgt gacattttcg gatgataagt acgcagtggt tcagcgatga
     4381 tctcgccgat ggtcatacgc gggtttagcg atgccaacgg atcctggaaa atcatctgaa
     4441 tatcactgcg aacggcacgc cattcatcgg gcttcatgcc cagcaactct ttacctaacc
     4501 aggcaacatg accgccggtc gctttaacca ggccaatgat ggcgcgagca aaggtggact
     4561 taccgcatcc cgattcccct accacaccta atgtttcccc ttcatacagg cgaagcgtga
     4621 caccatcgac ggctttgaga gttttcggcg gttgccagaa ccactgtttg ccatctttga
     4681 tttcaaagtg cactttcaga tcggcgattt cgaggaggac ttttcttcct tcggttacag
     4741 cattcataac agttcctcca ccggtttaaa gcaagcacgc agacggccag gcgtaaactc
     4801 ttccagcggc ggtgcgctac tacaaatttc catcgcatgc ggacaacgtg gctggaacgg
     4861 gcaacctttc ggtaatcgca gcaggtttgg cggattacca gggatggtca acattgtttc
     4921 accttccgca tcgagacgcg gcaccgcgtt gagcaaaccg atagaataag gatgaacggg
     4981 ttgataaaag acatcgcgcg cgttgccata ttccatcgtg cgcccggcgt acattaccag
     5041 cactttgtcg cagatccccg ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt
     5101 attaaattcc cgcttcagtt catttaatag cgtcatgatc tgcgcctgta cggtgacgtc
     5161 cagcgcagta gtgggttcat ccgcaatcag cagcttaggt cgacatagca atgccatcgc
     5221 aatcataact cgctgacgca tgccaccaga aaattcgtgc gggtacattt tcatgcgttt
     5281 acgtgcttcc ggcattttta ccgcatcgag catccgcacc gactcttcaa acgcctcagc
     5341 tttgctcatg ttcttatgca gcatcagcac ttccatcaac tgctcaccga cgcgcatata
     5401 gggattcaac gaagtcattg ggtcctgaaa aatcattgag atttgttcag cgcgcagttt
     5461 attgagttca cgctctggca aattgaggat ttcacgccca ttgaaggttg ccgatccgcc
     5521 aatacgtccg ttggcagcca acaggcccat caacgcaaat gcagtttgcg atttacccga
     5581 accagactca cctacaatgc ccagcgtttc tccggcacgt agagaaaaat tcaaatcatt
     5641 gaccgccgtg acatcgccgt ccggggtact aaaggtgaca cgcaaatctt tcacgttcag
     5701 cagtacgtca gcctgttgtt gtgcgagcgg cacagttgca gtttcaatta cgctcatggc
     5761 tgcactcctt aacgatcttt cgggtcgaga gcatcacgca agccatcgcc gataaagttg
     5821 aaacaaaaca gcgtcaccac gaggaatccc gctgggaaca acaataacca tggagagact
     5881 tccatcgagt tcgcgccatc actcagcaat gcaccccagc tgcttaacgg ctcttgcgta
     5941 cccaacccca ggaagctaag gaaagattca aagaggatca tgctgggcac cagaagtgat
     6001 gcgtagacca ccaccacacc gagtacattc ggcacaatgt ggcgaataac aatgcccgat
     6061 gtcgatacac cgccaacttg tgccgcctca ataaactctt tgcgcttcag actcagggtt
     6121 tgtccacgca caatacgagc catatccagc caggaaacca tgccaatcgc cacgaaaatc
     6181 agcaggatgt tttgaccgaa aaaggtcaca agcaaaatga cgaagaacat gaatgggaag
     6241 gagttgagga tttccagcag acgcatcatt accgaatcca ctttaccgcc cagataaccg
     6301 gaaagcgaac cgtaaagtgt ccccacgacc actgccacca gtgccgcagc aacaccgacc
     6361 atgagtgaga tacgcccgcc aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca
     6421 gtaccaaagt agtgaccgga ctccatatcc ggggcgctgg acatcatcgc ccagtcagta
     6481 tcgtcatagg caaactgcga aagcatcggt gccaggatta caaataacgc gatcagcacc
     6541 agcactatca gactggcaac cgccgcacgg ttatgcataa aacgtcgacg tgcgtcctgc
     6601 cacaagctgc gcccttcgac ctccagcttt tcactgaaat tttccagcgt ctcgctgttt
     6661 ttcttactta acatcatcgc gagctccagt atcagtaacg gattttcggg tcgataaccg
     6721 catatagcac atcgacaatg gcattaaaca aaatggttaa agcaccaacc aggatggtca
     6781 ggcttaacac taaggaatag tcacggttca atgcgccatt aacgaacagt tgtccaatcc
     6841 ccggcaaacc ataaatggtt tcgataacca tagaaccggt aataatgccg acaaatgcgg
     6901 ggcccatata agagagcacg ggtaacagag caggttttaa tgcgtggcgt aaaatgatcc
     6961 gccgcatagg taaccctttc gcccgggcag tacgaataaa gttggagtgt aatacttcaa
     7021 tcatagagcc tcgggtaata cgcgcaatac tggcgatata agcgagtgac aacgccacca
     7081 tcggcaatat catgaattta agcgccccac cattccagcc accgcccggc agccaatgca
     7141 aaatgatcgc aaatatcatg actaataatg gcgcaaccac aaaactgggg ataacaaccc
     7201 cggtcattgc cagccccatc acggtatagt cccatttggt gttttgtttt aatgcgacaa
     7261 taacgccagc actaacaccc agtattaccg caaggaaaaa tgcggcggct cccagtttgg
     7321 cagaaacggg aaaactggat gcaaccaggt cattgaccga ataatcttta tatttaaacg
     7381 atggaccgaa atcaccgtgc gccagttgtt tcaggtagct gaaatactgt gtcatgatcg
     7441 gatcattaag atgatatttc gcttcgatat tggccatcac ttccggcggt aaagtacgtt
     7501 cgccggtaaa agggcttccc ggcgcgaggc gcatcataaa gaacgaaata gtaataagaa
     7561 taaatagcgt cggaatcgct tccagacagc gacgtagaat aaattttaac attgccctac
     7621 cttctggcct gtgccttctg taatgcgata aaaatcagac accgtggagc aggacactcc
     7681 tgccccacgt attgccatta gtgcttcaca atgtacatat tccgggtgta ggtattatcc
     7741 agcggatctt tgccggtata gccaccaacc cacggtttca ccagacgcgc attcacgtag
     7801 taataaacag gaacaatggc cgaatcctta tccagctgtt gttctgcttt agtgtacaga
     7861 gctgtgcgct gcgcctcgtc agtcactttc agcgtttccg ccataatgct gtcaaaggcc
     7921 gggctcttat aatgcgcggt attcatcgag ctgttcgaca gcatggtgtt caggaaggaa
     7981 gttggttcgt tgtagtctgc acaccagcct gcacgggcca catcaaaagt gccctggtga
     8041 cgggtgtcga ggaacgtttt ccactcctgg ttaaccagtt tgacgtttac accaatgttt
     8101 ttcttccaca atgaagaggc agcaatcgcc agctttttat gcagatcaga ggtgttatac
     8161 agcaggttga tggtcaacgg tttgtctgcg gtataacccg cttcagccag cagttttttc
     8221 gcttcttcgt tacgtttttc ctggctccag ccaaaccatt ctggctgagt caattttgcg
     8281 ccatcagtat acggtggagt gtaaccatag gcgggcatgt cgccctgcgc tttcacttta
     8341 ttaacaatga tatcgcggtc catacctagt ttcagtgcgg tacgcacacg cacatcgttg
     8401 aatggcggtt tctggttgtt aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga
     8461 acttcgtccg ggatctcttt tttcagcttc tggaacaatt cgatcggcat gttgttatac
     8521 gtcatgtcga tttcaccact acggtagcgg ttgacatcgg taacttcaga agcaataggc
     8581 aaataggtta cctggttaat aacggttttc gcgttgttcc agtaggtcgg gctgcgttca
     8641 agaacgattc gttcgtttac gacccaatct tttaaggtat aggcaccgtt ggtgacgata
     8701 ttaccaggct gggtccattt ttcgccgaat ttctcgatag cggcttttgg caccggtgaa
     8761 gttgatgggt gaacaagtaa tttatagaag tacggaacgg gttcacttaa ggtgacttct
     8821 aatgtgtgat catcaatagc tttcacgccg agatcggtaa tcggtttttt cccttcaaga
     8881 atttcatcaa taccggcgat atgcccatat tgcagataac tggcatacgg agaagcagtg
     8941 ttcggatcaa cagaacgttg ccagctatac acaaagtctt gtgctgtgac tggcgtgcta
     9001 tcagaccatt tcgcatcttt acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc
     9061 caggattcag cgacgccagg tgctggatga ccgtcaagat cgctgaccag taagccttca
     9121 aacaggtctc ggctgatatt agactccgga acaccttcaa ttttgtgcgg atctaatgac
     9181 tgaacttctg aaccattgtt acgtaccagt gtttgttttt ccgccagtgt gacgcctgcg
     9241 ggtacatcag ctgccagcgc gacattccct gccattagcg cagccagaac gccagctgct
     9301 actaaacttc tcttggtgat gttggtcatt gttttttgga ctccctcatt ataattactg
     9361 gtattaacca gcatgtgtaa tcccggattg ggaccctgca caggctcgga gactttctgc
     9421 tgtcaggtta ctttatcgtt acgtattact atcaccgact ttatttattc tggtgattca
     9481 gacgatcacc ttatgtcgat tcgtactgtg tctcccctgt cgagacacat tattctttcg
     9541 cgacgctctt taattgttaa ttattctcat taccaataaa gaatcgtctg gcggttgccc
     9601 ggaaagtacc aaatgcggtt cttactcgcc aatacatttt gcaaaattgt taagcaattc
     9661 tcttttattg accgtatcag aatctatagc ttgcatccat gccttaacaa acatctttca
     9721 attcaatcaa atagagattg gctaagcatg tctatctttt ctgattacag ttccagtagt
     9781 gaaatgcaca ataatttaac aattgattat tatttagcac tttcctctac caaaggcagt
     9841 ggaattacta atatcatttc aattattctt cagcaagccc aggattatga tgtggcgaaa
     9901 ataacatgat ggtgaggaga agcagttaag gaaatcctta taactaactg taatttgaga
     9961 agaatatttt tacctattcc ttttgctttt gataactgcg cgggaacaaa gtaaaaataa
    10021 tgacgaagta aacggagcat ggcagctccg tttcatttga aaggaattaa ttaagcaggc
    10081 cggggaaaat ccccttaata ccagtaacga taaattcaat ccccaatgcc atcagcaata
    10141 gccccataat acgcgtaatc acgttgatgc cggtctggcg taaaacccgt accagccacg
    10201 gtgccatgcg gaacaatccc cagcaacata aagcgaacaa tgcaatagcc acaaagaaac
    10261 caaacagata gctaatgctg tgataacgcg taccccagac gatggtagaa ctgatcgccc
    10321 ctggccccgc catcaacggc aacgccaatg gcaccacacc aatgctttca cgtaccgcgg
    10381 tttctgattt ttcttgcttg ttctgtttat cctcgccaag cttgccgctg atcatcgaca
    10441 tcgctatcgt caccaccagg atgccccctg cgatacggaa cgaatcaatt gatataccaa
    10501 aaagttgcag aatcgtgtcg ccgagaaaaa gcgagatcca caagataatg gccacagaca
    10561 ggttggctgt aaggttagtt ttgtttcgcg ctgccgctgt ctgataactg gtcatgctga
    10621 taaagacggg aataatccct accgggttga ccagcgcaaa taacccgatg aaaaatttga
    10681 agtaaacggg aaaatcaaaa aaggtctgaa tcacggttag ctccgaagca aaagccggat
    10741 aatgttagcc ataaattaag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
    10801 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
    10861 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
    10921 aagcgatgct gaaaggtgtc agcttagcaa aaatttgatt tggatcacgt aatcagtacc
    10981 cagaagtgag taatcttgct tacgccacct agaagtgacg cattagagat aataactcta
    11041 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
    11101 ataggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
    11161 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
    11221 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
    11281 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
    11341 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
    11401 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
    11461 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
    11521 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gtctgaagac
    11581 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgccacca acaaagcggc
    11641 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
    11701 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
    11761 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
    11821 cggtgtaggc gcgggcaaca ctccagttgt tatcgatgaa actgctgata tcaaacgtgc
    11881 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
    11941 gtctgttgtt gttgttgact ctgtttatga cgctgtacgt gaacgttttg caacccacgg
    12001 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaacgg
    12061 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
    12121 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
    12181 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
    12241 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
    12301 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
    12361 aacggctcgt atcctgatta acaccccagc gtctcagggt ggtatcggtg acctgtataa
    12421 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tgggggggta actccatctc
    12481 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
    12541 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
    12601 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
    12661 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
    12721 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
    12781 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
    12841 cgccgcgaag attatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
    12901 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
    12961 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
    13021 tgtaactgac gacgctactg gtcagaaata tccgctggca gactatgcgc tgactccgga
    13081 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
    13141 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
    13201 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
    13261 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
    13321 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
    13381 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
    13441 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
    13501 ggctcgccgt cgttatgctg aaattgccga ccacttgggt ctgagcgcac cgggcgaccg
    13561 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
    13621 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
    13681 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
    13741 gatctccgag ctgaaacaga tcctgc
//
LOCUS       DAZPAC010000107        13760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZPAC010000107 DAZPAC010000000
VERSION     DAZPAC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13760
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.107"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..80)
                     /locus_tag="ACHUPM_002589"
     CDS             complement(<1..80)
                     /locus_tag="ACHUPM_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870326.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGT"
     gene            255..2021
                     /gene="adeD"
                     /locus_tag="ACHUPM_002590"
     CDS             255..2021
                     /gene="adeD"
                     /locus_tag="ACHUPM_002590"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR0870327.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="ACHUPM_002591"
     CDS             complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="ACHUPM_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR0870328.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="ACHUPM_002592"
     CDS             complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="ACHUPM_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR0870329.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="ACHUPM_002593"
     CDS             complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="ACHUPM_002593"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR0870330.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="ACHUPM_002594"
     CDS             complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="ACHUPM_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR0870331.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="ACHUPM_002595"
     CDS             complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="ACHUPM_002595"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0870332.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="ACHUPM_002596"
     CDS             complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="ACHUPM_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR0870333.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="ACHUPM_002597"
     CDS             complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="ACHUPM_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR0870334.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9842..9931
                     /gene="tisB"
                     /locus_tag="ACHUPM_002598"
     CDS             9842..9931
                     /gene="tisB"
                     /locus_tag="ACHUPM_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR0870335.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(10055..10129)
                     /gene="ysdE"
                     /locus_tag="ACHUPM_002599"
     CDS             complement(10055..10129)
                     /gene="ysdE"
                     /locus_tag="ACHUPM_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR0870336.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10211..11395
                     /gene="emrD"
                     /locus_tag="ACHUPM_002600"
     CDS             10211..11395
                     /gene="emrD"
                     /locus_tag="ACHUPM_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR0870337.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11403..11900)
                     /locus_tag="ACHUPM_002601"
     CDS             complement(11403..11900)
                     /locus_tag="ACHUPM_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR0870338.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11897..12259)
                     /locus_tag="ACHUPM_002602"
     CDS             complement(11897..12259)
                     /locus_tag="ACHUPM_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR0870339.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12249..12596)
                     /locus_tag="ACHUPM_002603"
     CDS             complement(12249..12596)
                     /locus_tag="ACHUPM_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR0870340.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12705..13154
                     /gene="yidI"
                     /locus_tag="ACHUPM_002604"
     CDS             12705..13154
                     /gene="yidI"
                     /locus_tag="ACHUPM_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR0870341.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13201..13359)
                     /locus_tag="ACHUPM_002605"
                     /pseudo
     CDS             complement(13201..13359)
                     /locus_tag="ACHUPM_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13403..>13760)
                     /locus_tag="ACHUPM_002606"
     CDS             complement(13403..>13760)
                     /locus_tag="ACHUPM_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0870342.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPA
                     YTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRSLKQEPTEMIQATA"
BASE COUNT         3267 a         3816 c         3571 g         3106 t
ORIGIN      
        1 gtcccatgct gaggtagttt aaataatcgc gaaagcgtcc ctgattcatt actcacgtaa
       61 tcggtattgt cattattcat ttttttgtcc attggaggag atttaatccc ttgcttttgt
      121 aaaacaaatg acaacaacgg ttcagtgata attatcacat ttcaattgca cattaatgga
      181 tattctttaa taatctcgcg acgtttcttt atgataaata ataatcaaat tgataaaatc
      241 aaaatgagaa aaatatgaat aattctatta accataaatt tcatcacatt agccgggctg
      301 aataccagga attgttagcc gtttcccgtg gcgacgctgt tgccgattat attattgata
      361 atgtctctat tctcgacctg atcaatggcg gagaaatttc cggcccaatt gtgattaaag
      421 gacgttacat tgccggtgtt ggcgcagaat acgctgatgc tccggctttg cagcggattg
      481 atgctcgcgg cgcaacggcg gtgccagggt ttattgatgc tcacctgcat attgaatcca
      541 gcatgatgac gccggtcact tttgaaaccg ctaccctgcc gcgcggcctg acgaccgtta
      601 tttgcgaccc tcatgaaatc gtcaacgtga tgggagaagc cggattcgcc tggtttgccc
      661 gctgtgccga acaggcaagg caaaaccagt acttacaggt cagctcttgc gtacccgccc
      721 tggaaggctg cgatgttaac ggtgccagtt ttacccttga acagatgctc gcctggcggg
      781 accatccgca ggttaccggc cttgcagaaa tgatggacta ccctggcgta attagcgggc
      841 agaatgcgct gctcgataaa ctggatgcat ttcgccacct gacgctggac ggtcactgcc
      901 cgggtttggg gggtaaagaa cttaacgcct atattactgc gggtattgaa aactgccacg
      961 aaagttatca gctggaagaa ggacgccgga aattacaact cggcatgtcg ttgatgatcc
     1021 gcgaagggtc cgctgcccgc aatctcaacg cgctggcacc gttgatcaac gaatttaaca
     1081 gcccgcaatg catgctctgt accgatgacc gtaacccgtg ggagatcgcc catgaaggac
     1141 acatcgatgc cttaattcgc cgcctgatcg aacaacacaa tgtgccgctg catgtggcat
     1201 atcgcgtcgc cagctggtcg acggcgcgcc actttggtct gaatcacctc ggcttactgg
     1261 cacccggcaa gcaggccgat atcgtcctgt tgagcgatgc gcgtaaggtc acggtgcagc
     1321 aggtactggt gaaaggcgag ccgattgatg cgcaaacctt acaggcggaa gagtcggcga
     1381 gactggcaca atccgctccg ccatatggca acaccattgc ccgccagcca gtttccgcca
     1441 gcgactttgc cctgcaattt acgcccggaa aacgctatcg ggtcattgac gtcatccata
     1501 acgaattgat tacgcactcc cactccagcg tctacagcga aaatggtttt gatcgcgatg
     1561 atgtgtgctt tattgccgta cttgagcgtt acgggcaacg gctggctccg gcttgtggtt
     1621 tgcttggcgg ctttggactg aatgaaggtg cgctggctgc gacggtcagc catgacagcc
     1681 ataatattgt ggtgatcggt cgcagtgccg aagagatggc gctggcggtc aatcaggtga
     1741 ttcaggatgg cggcgggctg tgcgtggtac gtaacggcca ggtccaaagt catctgccgt
     1801 tgcccattgc cggcctgatg agcaccgaca cggcgcagtc gctggcggaa caaattgacg
     1861 ccttgaaagc cgccgcccgt gaatgcggtc cgttacccga tgagccgttt attcagatgg
     1921 cgtttctttc tctgccagtg atccccgcgc taaaactaac cagtcagggg ctatttgatg
     1981 tcgagaagtt tgccttcact acgctggaag tcacggaata ataaaaaagc ccggcgtcat
     2041 gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa ctgctgaatt tttttctcgc
     2101 ggcggatttt gcgttcttcc atcaccgcca ctatcgccat caggcagata caaccaatcg
     2161 cggcgatatc aagtgcggcg aaggtgcctg cccagccggt aaggccgaat accggcgtcc
     2221 catcggcaat cattcccaga cctaacttgg caaagctgtc accaatcagg taagcaaagg
     2281 tgcctttaat accatcggca gcgccaatcg cttttttagg tacaaagcca acagcagcca
     2341 caccaatcaa caattgcggg ccaaagacca ggaaacccaa cgcaaagaga gaagccagat
     2401 agatatattc gttactggca tgttgataca caccgagcgt ggcgataatc agcgccagcg
     2461 cgatgcaggc caccaggcca cggcgaccgt tcgccaggtc agagagccag ccccacagca
     2521 gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc ctgaatcgcc accgctttag
     2581 agagtttcag ttcctggaac gcgtatacgg ttgaccactg gtcgatacca atacgtacca
     2641 catagaggaa aatgttggcg aagcacagca gccagatcac tttgtttttc agcacatact
     2701 caacaaagat ctgccacttg gtcatatcgg tagattctgt ctctttgtcc tcttcgctga
     2761 tctcctcgcc gaacagttct tcagctttgc cgaggccata agattccggg gagtcgctgc
     2821 cgtaacgcag gccgataaaa ccgacaatca gcgcgataat cgacgggaag ataaacatgc
     2881 cgatgacatg gccatcgaac aggtaatttg ccccgaacag cgccacgcct gctgcgcctg
     2941 caccgccaag gttgtgagaa atattccaga aaccgaggaa tgtcccgcgt ttacgacgcg
     3001 gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc ggtactctgg aaaaagccgc
     3061 ttaaggcgta gaaggcaatc atcaggaaca ggctaaccga gccgctgccc atactggcac
     3121 tgaagcccag catacaaatg gcagacagga tcagcatgaa cggcaggaat tgtttggtgt
     3181 ttttgccgtc ggcgtagtag gaaaccagcg ttttacccac gccataagtg atggagaaac
     3241 ccaggccgat catccccagc tgcgtcatgc tcaacccgta ggtcgagatc atgtcgttct
     3301 gcgcgatgtt aaagttcttg cgaatcaggt acatcgtcag gtagccgata aagaccacca
     3361 ggtaggattg catgaacggt ttgaaccaca ttttgcgccg cacttcgagc ggaaggtcca
     3421 gggtcggctt gcgaacctgg tttaagaaag ccagcatggg ttactcctga aatgaatacc
     3481 tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg cctgagatag cgtccaggca
     3541 aaacctggaa aatttcttag ttttgccgga tatgaagtga aaaggtgaga tgcatcacgc
     3601 ttcgcgcggt gtctgggcgt tcaaaaaggg cagtaacagc agtgcggaaa tcccggcggc
     3661 gatagagata accacaaaaa atccgctcca gtgccaggta tcgagtactt tcgccagcgg
     3721 ccaaccagca agcgacgccc ccagataagc aaacaagccg acaaaccccg tcgccgcccc
     3781 tgccgcctct ttgtgggaac actctgccgc cgccataccg attaacatct gtgggccaaa
     3841 gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc acgtagctgg caaatggcat
     3901 cagccacagg gagccgactg aaagcaaaat tccggcggcg aaaatcaaat tcatcggccc
     3961 tcggttgccg ttaaacaatt tgtccgagcc ccaacccgct accagcgcac cgataaatcc
     4021 acccagttca aacatcgtca ctgccgtatt cgccgtgacc agatcgacgc ccagcgtctc
     4081 ggacatatac aaattgcccc agtcgttgat cgccgcccgg accacataga ccagcacata
     4141 gcaaaacgaa agcagccaga tatacggatt cagcaacaca tatttggtga ggatctcttt
     4201 acgcgtcagc cctgcccctt cttgttgttg agctatctcc agcgcgtcgt gtcgccattc
     4261 accgaccgcc ggtaaaccta acgcctgcgg gcgatcgcgc agccgccagc agagaaaaat
     4321 tcccacgacg atcgccatac aaccagcaat catcatcccg gcacgccagc cgtaatgtag
     4381 tgcagccgct gccatcacaa tgggaatgag tgcgccgccg acgttatgcg ccgtgttcca
     4441 taatgcccac caaccaccgc gctcggtacg tgaataccag gccgttaaca gacgcgcaca
     4501 caccggtgaa ccccagccct ggaaaaaggc gttcagcacc cagagtacgg caaacgccca
     4561 tagcgacgtc gagaagccaa acagaatgtt gataatgccc gtggcgataa gccctatccc
     4621 cataaaataa cgggcatttg agcgatcgct gacaatgccg gagacaaact tcgacacgcc
     4681 ataggtaatg taaaacaggg tcgctaacag gccgatatcg ctacggctga gcacgccgtt
     4741 agcaaggatt tctggtacgg cggcgttaaa acttttccgc gtgaagtaaa acagcgcgta
     4801 acccagccag atggtcagca gaatatgccg acgccagtag cgatagcggg catcaatttc
     4861 atatttatca gccattaatg gcgcatcggc aggcgctttc agaaacggca acatcatcaa
     4921 accttagaca tagcgttgag gtagagaaac gctgacacgc gtgccgtgca gacaggaaat
     4981 gtgtaatgtg ccacccagcg ccgttacgcg ctcgcgcatt ccggtgaggc caaaaccttg
     5041 ttgcccggaa cccggcggca aaccgctgcc atcgtcttca ataaccagca tcaaccgttc
     5101 atcctgctgc cagccttgca gggtgaccgc gctggcatca gcatgtttca caatgttgtt
     5161 cagcccttcc tggcagacac gaaacagcgt cacgcgctgg ttttcgctta acgctgattc
     5221 atcgattcgc cattcgagat ggctgacaat accgcgccct tccagctcca tttcccgcat
     5281 cagtgagcgg atggcctgct ccagggtgag atcatccaac tggcgcggac gtaaccgacc
     5341 caacaaacgg cgcaccgcgt cgtaaacgcc cagcgatagt tgttcgatga gttgcccgct
     5401 ctgcttcacg ctggcgttat ctgccgccag ccgctgaaca atgcccgcct gagtacgaat
     5461 agcagtgatg gtctggccga tatcatcatg cagctcacgc gccacatcac ggcgcacgct
     5521 ctcttcggtt tccagcaacc gttcagccag atgctgattg cgcgccagtt ccttttgcag
     5581 cgactggtta agttcacgca accgctggat gccagcgcca agcaacaacc ctgtcagact
     5641 ttgcaccagc agcgagagca ataaatccac cggatgatcg cgccaggttt gactggcgat
     5701 cagcgcgatg gcgttcatca acgtcgcaat cagcgcccct tgccaaccat agtgccaggc
     5761 cagcgcgata atcggcagca ccagacagaa tggcgtaaag cgcgacagtt cgtccggcaa
     5821 tcccaactgg agccagagac tgataacaaa cagcagcaag taccagacca gatgtcgccc
     5881 gcgccagttg attggctgag aaaccagtga cgggccgagc ggcagccagg tgttattggc
     5941 gagatagtgc cagaacacca gacatatcgg ggccagcgtc aggccgccag ttaaagtcag
     6001 caacagcgca ttccacgact ctttgccgtg ccaaagccag ggcagcgact gcaacaacgc
     6061 cgccgccgtt aacgccgccc cctgtaacag caaggtgcgc cagtcacgct gatggcgata
     6121 gcgcgagatc agcgctacgg gcagtaacgt cagtaaacta ccgatcatca ataacggaaa
     6181 atgggttaaa ccgaccgcct gcgttagcca gtaaatcagc agccactccg cacccagcaa
     6241 tacgggccag tatccgcgcg ggcattgcag cattagcccc agacgcagac caaacggaaa
     6301 taacagcacc gccatatcag ggcgctcaac cagatgcagg ctgatactcc acaggcaaaa
     6361 ccatgcggca gagaagataa aaaagcaggc aataacggta attaagcggg agaacaacgt
     6421 cttcatcacc agccatcaaa catgcggcgc gccagctcta cgtcgttact gacgcccagt
     6481 ttttccatca gattggcgcg atggacgtgt accgttttcg gtgacaagcc cagttcggcg
     6541 gcaatctctt tcaccgccat tccttgcgcc agtttttccg ccacctggcg ttcacgtttg
     6601 gttagcgggt cctgacgacc ggatgccagt ttaatggcaa tatccggcgt cagataacag
     6661 ccacccgtgg caaccgtatg caccgcagca atgagttcat ccgggctaca gcgtttggaa
     6721 agaaagccgc gtgcccccgc gttaagcgcc tgctcaacca gcgcaggact gtcgtgaacg
     6781 gagagcataa tcgtcgccat acctttcggc agctggctta gcagctccag accggagata
     6841 tcgggcatgg agatatcgca aatacacacc tgcacaccgc gccccggcag ccccgccagc
     6901 gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat caggttccag ccccagcagc
     6961 tgcgcaaagc cggagcggac gatgaggtga tcgtctataa gggcaacggt gatcatggtc
     7021 ttgtcctggc gggtaaaaaa aaacgcgctt accttaacga taagcgcgat gttgttcaag
     7081 ccttgagcgg ttactgaaaa aacaccgcga tcttgttaaa catcgtcgga tcggactgat
     7141 tacgctgcac tttcacgaca tcttccagct tatcgatttg gcttatcatc tgctccagac
     7201 gctggtcgtc attgaccagt agccagatat ggcttttgtc gctgtcctga atcggcagac
     7261 aaagaatgcc ttcaacgtta aaagcgcggc gggcaaaaag gccacaaacg tgggtcatta
     7321 cgcccggatg gttgcgaacg gtgagctcca gaattacgtt gtcatgagtt gtgttttgca
     7381 tggcttattc ccccaccatt tcagtattcg ccgcacctgg cggcaccatc gggtaaactt
     7441 tttcttcggc atcaatgcgc acatggatca gcgccgggct agggcgattg atgatttcct
     7501 gcaatgcagc ctgcggatcg gcttcgttat tcaaatcaca ggtttcgagg ccgaatccgg
     7561 cggcaatctg cataaagttg attttgcccg gataggtggc ggcaaaaacg ccttgctcgt
     7621 agaacagact ctgttgctga tgcaccagcc ccagcgcttc gttgttcatc agaatgattt
     7681 tgacatccag ctgattttca ctggcggtcg ccatctcctg aatattcatc atcaggctgc
     7741 cgtcgccgga gaaacacaac actttgcgat ccgggttcgc cagcgccgcg ccaatcgccg
     7801 caggcaggcc aaaacccatc gtgcccagcc caccggaggt cagccactgg cgtgggcgat
     7861 tgagcggata agcttgcgcg gtccacatct gatgctgacc cacatcggtg gtgataatcg
     7921 cattgtcatc gacacaggcg gcaacggcgt tgatcaggcc gtaatggctt aatggatcgc
     7981 acgctttcgg gattggacac gggaattcgc gctgcaaatc cgctaccaac tggtgccact
     8041 ctgcacgcgg ttgcgcttcc acctgcggga tcaattgcgc cagcacgtca tcaacatccg
     8101 cctgaatcgc cacatgcggc tgcttgattt tacccagctc tgcgcgatcg atatcgacat
     8161 gaataatttt ggcattcgga cagaactgct cggttttgcc aatcgcccgg tcatcaaaac
     8221 gcgcaccgag cacaatcaac aaatccgcct cctgcaaaat atagttggta ctgcgcacgc
     8281 cgtgcatccc cagcataccc agcgacaacg gatgcgcttt tggcagcatg cccagcgcca
     8341 ttaaagtcat ggtggtaggc agttgcgctt tctccgccag ttcacgcacc cgcgcaggcg
     8401 cattgatcac accaccaccc agataaagca ccgggcgttt ggcagcgtta atcattgtag
     8461 ctacgtcacg aatgctttct tcgctaaagg cgggtgcagc ggctttttct gccaccgcgg
     8521 gctgagcttc aatctcaaaa accgccgttt gtacatcctt aggaatgtct atccacaccg
     8581 ggccagggcg gcctgattgc gcaatgcgga aggcatcgct catgacctgc gggagttctt
     8641 cgatatgtct gaccagatag ttgtgtttgg tgatggggat agagatgccg taggtgtcga
     8701 cttcctggaa ggcgtcggtg ccgatcatcg aggcgggaac ctgaccagtg atgcaaatca
     8761 gcgggatgga gtccagccgc gcatcggcaa tggcggtcac caggttagtc gcacccggtc
     8821 cgctacaggc catacagacc gccggtttac cgtcggtgcg cgccattccc tgagcgataa
     8881 agcccgcgcc ctgttcatga cgggccagaa tatggcggat ttgcgtgctt tggcttaagg
     8941 catcgtaaac aggcaggata gaaccgcccg gaatgcctgt cacaatctta atgccctgct
     9001 gttccaggaa atgaacgata aattctgcgc cggtaaagcg cttacgcgtc aatgttgtgc
     9061 ccgaacttgc catgctccag tccttttctt ctgggccgac tttccgggag gtgcttaaac
     9121 gaaaaacccc gcccggtttg cgccgacggg gttttggaat cgtgtgttgt tccagtccct
     9181 acggcgcatt gccgacgacc accaccacac gcacgacgac cactgcggcg gatggcgcag
     9241 ttggtagtag ttttgcgttg aacatggaag tagtcattgg ggaccttgtt ggttttgtgt
     9301 ttaacaatat ttatacaagc acagctttac aggggagaca atggaaaatt tttcagcaag
     9361 ggaaaattga ggggttgatc acgttttgta ctgaattgca gataacaaaa aaccccgccg
     9421 gagcgaggtt tcgtcagtcg cccgcggctg gtaaccgcaa agcacactgt attatgtcaa
     9481 cacagaaagt atacgtgttc cgcgcagaac gcgcaatttc ggcacgaatt ttgacgtatt
     9541 tagtgcatag ttgagtatcg atcacagttt gcgttttgtc caaatattgc tgtttattta
     9601 tacagtaaac ttctataata tcactgtacg caatgtgtta tgcgggggcc gcatcgttac
     9661 ccggcgcact aagtcctggc tgaaacgggt ggtgccgtca gcgccttaac cccgcgtgag
     9721 cacactgtgt tatgtcaaca agcacaaagt ttctccttga gataccgcgt gcacaacagc
     9781 tggcaacagg cagcggaaag gtacgtcagc tggcagtgct cctgaaccac aggagacgcg
     9841 tatgagcctg gtggatatcg ccattcttat cctcaaactc attgttgcag cactgcaact
     9901 gcttgatgct gttctgaaat acctgaagta attcagattc aagtcgcacc aaaggggagc
     9961 gggtaaccgc tcccctttta tatttagcgt gcgggttggt gtcggatgcg atgctgacgt
    10021 atcttatccg ccctaccatc tctcccggca acatttattg ccgcttttgt ttacatattc
    10081 tgccgctaaa caactcccca ttcctggcgt atatctggct aacattcatc aatgtgatag
    10141 attcctctcc cgcatttatg ggaatgcgta gtgacttact ctaattattt ttataaaagc
    10201 atccgtgata atgaaaaggc atagaaacgt caatttgtta ttgatgttgg tattactcgt
    10261 ggccgtcggt cagatggcgc aaaccattta tattccagct attgccgata tggcgcgcga
    10321 tctcaacgtc cgtgaagggg cggtgcagag cgtaatgggc gcttatctgc tgacttacgg
    10381 tgtctcacag ctgttttatg gcccgatttc cgaccgtgtg ggtcgccgac cggtgatcct
    10441 cgtcggaatg tccattttta tgctggcaac gctggtcgcg gtcacgacct ccagtttgac
    10501 ggtattgatt gccgccagcg cgatgcaggg gatgggcacc ggcgttggcg gcgtaatggc
    10561 gcgtactttg ccgcgagatt tatatgaacg gacacagttg cgccatgcta acagcctgtt
    10621 aaacatgggg attctcgtca gtccgttgct cgcaccgcta atcggcggtc tgctggatac
    10681 gatgtggaac tggcgcgcct gttatctctt tttgttggtt ctttgtgctg gtgtgacctt
    10741 cagtatggcc cgctggatgc cggaaacgcg tccggtcgat gcaccgcgca cgcgcctgct
    10801 taccagttat aaaacgcttt tcggtaacag cggttttaac tgttatttgc tgatgctgat
    10861 tggcggtctg gccgggattg ccgcctttga agcctgctcc ggcgtgctga tgggcgcggt
    10921 gttagggctg agcagtatga cggtcagtat tttgtttatt ctgccgattc cagcagcgtt
    10981 ttttggcgca tggtttgccg gacgtcccaa taaacgcttc tccacgttaa tgtggcagtc
    11041 ggttatctgc tgcctgctgg ctggcttgct gatgtggatc cccgactggt ttggcgtgat
    11101 gaatgtctgg acgctgctcg ttcccgccgc gctgttcttt ttcggtgccg ggatgctgtt
    11161 tcctctggcg accagcggcg cgatggagcc gttccccttc ctggcgggca cggctggcgc
    11221 gctggtcggc ggtctgcaaa acattggttc cggcgtgctg gcgtcgctct ctgcgatgtt
    11281 gccgcaaacc ggtcagggca gcctagggtt gttgatgacc ttaatgggat tgttgatcgt
    11341 gctgtgctgg ctaccgctgg cgacgcggat gtcgcatcag gggcagcccg tttaagcgca
    11401 cgtcaccgca gcatcgtcat cagctccatg ggagaacgat gctgctttat cagatcacgc
    11461 atcacccgca tatgcggtgc ggagtaagaa taaaacgcct gatagcccgc acaaagcacg
    11521 ctgtctggcg tctcctgccg agcgtgcggg cacaggcgat gccagtcgca cgcctggcag
    11581 tcacagcgcg gttgcgggtt ttcgctgcca caccattttt gtaaggtggc gtcaaaaagc
    11641 tcaatcgaga tacgactgat atctgcccgc acccaacact cgaacagggc aattaaaaat
    11701 ttcccccaca gctctgctgt caccgaagcg gccgtcagat cgccccggtc atcaaattca
    11761 agatagggaa tgagctgtac ggagcggatg ccccgctgtt tgaaattgtg ataaaaatcg
    11821 gcaggttgca gagcatcctt gtactctaca accagtttat gcctgtcctc ttcagcatca
    11881 atgacctgac ttcctgtcat gcgactctct caataaatac gataagttga tgtatatgcg
    11941 ttacagcaaa cagtattgcg agagataaca ccgcgaggga gatcaaaaat ttgtcacgga
    12001 ctacgtgaaa ttgggaaaaa tcgctattcg tgacatccat taaattgcga ttacgggtgt
    12061 agtgccagag gatcagcgcc acgatggcga gaatgccaat cgaaatccag aataacatgc
    12121 ccgcctggtg ccagttgtgt ttgatagcca acgccatcaa cgcgccgtag cccagcatgg
    12181 tacgaaacca cgccagtgat gtgcgctccg gctgcagtcc tgggtcggca atgcgtctgg
    12241 ctttgcggct atccggcata caacaccagt cccataacga tcaccgccac gaccatcaat
    12301 attaagctaa tgattaataa gctgttggtg tagggcaggt cctctttcag acgcatcgct
    12361 ttttcattgc gtaaccagcg caggtagcca tacattgcta acccgccgga aaacagacac
    12421 aacaacaacg ccagcagttc acgaatgacg ggcgtggcga aatctggcgc aagttgatca
    12481 agcccaaccc ccgccgccag aaatcctagt gcggtacgga tccacgccag aaaagtacgc
    12541 tcattagcca gcgagaagcg gtaatcgggc gcttctccaa ggcgggaaat tttcattgtt
    12601 gctcctttgc aatttcacta actctctacc tttagcggtt ttttttaaga atttcgtccg
    12661 cccgtttcct gatatgcttt tgccatccgc tacaaggagg tcagatgggt atcatagcgc
    12721 aaaataaaat cagttcattg ggcatgctgt tcggtgccat cgctttaatg atggggatta
    12781 tccacttttc attcggccct ttttccgcac caccgccaac gcttgaaagc atcgtggcgg
    12841 acaaaacggc tgaaattaaa agaggtcttc tcgccggaat aaaaggcgaa aaaattacca
    12901 ccgtggagaa aaaagaggac gtagatgtcg ataaaatcct cgatcaaagc ggtatcgcac
    12961 tggccattgc tgcgctactt tgcgcatttg ttggcggtat gcgtaaggaa aatcgctggg
    13021 gcattcgcgg cgcgctggtt tttggcggcg gaacgctggc tttccatacc ttgctttttg
    13081 gtatcggcat cgtgtgcagc attttgctga tattccttat cttttccttc ctgaccggcg
    13141 gttcgctggt ttaagcagcc tgaataatgc ccgccgggtg atcatcagcg ggcattatcg
    13201 tcagaacttc tgttttttct cttccacctt caccccttgt gtcggtaggc cggtgtcata
    13261 gtcacgtacc accggcgaat agccatcttg tgggcgtgga cgaaatgccc ccatccagcg
    13321 cggccgtgca tctttacgcc acggacgcag gctccattgt tgacatcctc cacgccctga
    13381 aggacgtgga cccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct
    13441 tcaacgagcg gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta
    13501 aaatgttacg agcgccattt acatcggcgt tcgctgtata tccacatacc tggcatctga
    13561 atttactttg tgacaggcga ttttctttcg ctgtatgacc acagcacacg caacgctggc
    13621 ttgtgtacgc tggcggaaca gcaagcacct gaccaccacg ccagagctgc ttgtacgcaa
    13681 gctggcggcg catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg
    13741 cccggacatt gcgccccggc
//
LOCUS       DAZPAC010000108        13313 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZPAC010000108 DAZPAC010000000
VERSION     DAZPAC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13313
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.108"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(135..755)
                     /gene="sodA"
                     /locus_tag="ACHUPM_002607"
     CDS             complement(135..755)
                     /gene="sodA"
                     /locus_tag="ACHUPM_002607"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR0870343.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            1040..2074
                     /gene="rhaT"
                     /locus_tag="ACHUPM_002608"
     CDS             1040..2074
                     /gene="rhaT"
                     /locus_tag="ACHUPM_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFR0870344.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(2071..2919)
                     /gene="rhaR"
                     /locus_tag="ACHUPM_002609"
                     /pseudo
     CDS             complement(2071..2919)
                     /gene="rhaR"
                     /locus_tag="ACHUPM_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(2993..3829)
                     /gene="rhaS"
                     /locus_tag="ACHUPM_002610"
     CDS             complement(2993..3829)
                     /gene="rhaS"
                     /locus_tag="ACHUPM_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR0870345.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            4117..5586
                     /gene="rhaB"
                     /locus_tag="ACHUPM_002611"
     CDS             4117..5586
                     /gene="rhaB"
                     /locus_tag="ACHUPM_002611"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR0870346.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            5583..6842
                     /gene="rhaA"
                     /locus_tag="ACHUPM_002612"
     CDS             5583..6842
                     /gene="rhaA"
                     /locus_tag="ACHUPM_002612"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR0870347.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            7084..7908
                     /gene="rhaD"
                     /locus_tag="ACHUPM_002613"
     CDS             7084..7908
                     /gene="rhaD"
                     /locus_tag="ACHUPM_002613"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR0870348.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            7918..8232
                     /gene="rhaM"
                     /locus_tag="ACHUPM_002614"
     CDS             7918..8232
                     /gene="rhaM"
                     /locus_tag="ACHUPM_002614"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR0870349.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            8533..8979
                     /locus_tag="ACHUPM_002615"
     CDS             8533..8979
                     /locus_tag="ACHUPM_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR0870350.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            8990..10519
                     /locus_tag="ACHUPM_002616"
     CDS             8990..10519
                     /locus_tag="ACHUPM_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFR0870351.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVFLVCYLANDKALLGTQSGAGFLGAVVLGLAIGYFVFWFRKV
                     RLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAA
                     FLVGAMLAFDMGGPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAGLATFIAPK
                     LFTRQEKEAASSAIVVGATVATEPAIPYALAAPLPMITANTLAGGITGVLVIAFGIKR
                     LAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELVHE
                     H"
     gene            10509..11579
                     /locus_tag="ACHUPM_002617"
     CDS             10509..11579
                     /locus_tag="ACHUPM_002617"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR0870352.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            11579..>13313
                     /locus_tag="ACHUPM_002618"
     CDS             11579..>13313
                     /locus_tag="ACHUPM_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFR0870353.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMEL"
BASE COUNT         3130 a         3428 c         3539 g         3216 t
ORIGIN      
        1 aacgctgttt tatttttatc ggatcattgt ttttattttg aaattgatca caaaaaaacc
       61 accgccgttg gcgatggttc attttttaag ctgatatgcg gcctatacgc ctcattgcag
      121 caggcggcaa atgattattt tttcgccgca aaacgtgccg ctgcttcgtc ccagttcacc
      181 acgttccaga actctttaat gtagtccgga cggcggttct ggaatttcag gtagtaagca
      241 tgttcccaca catccaggcc cagaatcggg aagccggaag cgccagaaat agcttcaccc
      301 atcagcggag aatcctggtt agcagtagaa accaccgcca gtttatcgcc tttcagcacc
      361 agccatgccc agccggaacc aaagcgggaa gctgccgctt tttcaaattc tgctttgaag
      421 ttatcaacgg agccgaagtc acgttcgata gccgctttca ggtcaccctg cagggtggtg
      481 ccttttttca gacctttcca gaacaggctg tggttagcgt gaccgccagc gttgttgcgc
      541 agtacggttt tcttgtctgc tggcagctgg tccagtttgg tgatcagctc ttcaaccggc
      601 aggttggcaa attctggcag gctttccagc gccgcgttgg cgttgtttac gtaggtctga
      661 tggtgtttgg tgtggtggat ttccatggtc tgcttatcga agtgcggttc cagggcatcg
      721 taagcatacg gcagggatgg cagggtatag ctcatattca tctccagtat tgtcgggcgg
      781 ccgattgtta atgccgcgta agcagttggt tcattatagt taattaaatg atattgaaaa
      841 tgattatcaa tgccgtactt ttcgtaaggg tatggttttg caggaaaatg cccgagatgt
      901 gaagcaaatc acccacttaa tgccgtgatt gccagtaaat cgacaacggc ggcaacaggc
      961 gaaaggttaa tcgacagcac gatttttaca ctcatctcgt cggagatgtg aagcgacgaa
     1021 aaatgatgag gataagaaga tgagtaacgc gattacgatg gggatatttt ggcatttgat
     1081 cggcgcggcc agtgcagcct gtttttacgc tccgttcaaa aaagtaaaaa aatggtcatg
     1141 ggaaaccatg tggtcagtcg gtgggattgt ttcgtggatt attctgccgt ggaccatcag
     1201 cgccctgtta ctaccggatt tctgggcgta ttacagctcg tttagtctct ctacgctgct
     1261 gcctgttttt ctgttcggcg ctatgtgggg gatcggtaat atcaactatg gcctgaccat
     1321 gcgttatctc ggcatgtcga tgggaattgg catcgccatt ggcattacgt tgattgtcgg
     1381 tacgctgatg acgccaatta tcaacggcaa tttcgatgtg ctgattaaca ccgaaggcgg
     1441 acgcatgacg ttgctcggcg ttctggtggc gctgattggc gtagggattg taactcgagc
     1501 cgggcagttg aaagagcgca agatgggcat taaagccgaa gagttcaatc tgaaaaaagg
     1561 gctggtgctg gcggtgatgt gcggcatttt ctctgccggg atgtcctttg cgatgaacgc
     1621 cgcaaaaccg atgcatgaag ccgctgccgc acttggcgtc gatccactgt atgtcgctct
     1681 gccaagctat gttgtcatca tgggcggcgg cgcgatcatt aacctcggtt tctgttttat
     1741 tcgtctggca aaagtgaagg atttgtcgct aaaagccgac ttctcgctgg caaaaccgct
     1801 gatcattcac aatgtgttac tctcggcact gggcgggttg atgtggtatc tgcaattctt
     1861 tttctatgcc tggggccacg cccgcattcc ggcgcagtat gactacatca gttggatgct
     1921 gcatatgagt ttctatgtat tgtgcggcgg tatcgtcggg ctggtgctga aagagtggaa
     1981 caatacagga cgccgtccgg taacggtgtt gagcctcggt tgtgtggtga ttattgtcgc
     2041 cgctaacatc gtcggcatcg gcatggcgaa ttaatctttc tgcgaattga gatgacgcca
     2101 ctggctgggc gtcatcccgg tttcccgggt aaacaccacc gaaaaatagt tactatcttc
     2161 aaagccacat tcggtcgaaa tatcactgat taacaggcgg ctatgctgga gaagatattg
     2221 cgcatgacac actctgacct gtcgcagata ttgattgatg gtcattccag tctgctggcg
     2281 aaattgctga cgcaaaacgc gctcactgca cgatgcctca tcacaaaatt tatccagcgc
     2341 aaagggactt ttcaggctag ccgccaaccg ggtaatcagc ttatccagca acgtttcgct
     2401 ggatgttggc ggcaacgaat cactggtgta acgatggcga ttcagcaaca tcaccaactg
     2461 cccgaacagc aactcagcca tttcgttagc aaacgacaca tgctgactac tttcatgctc
     2521 aagctgaccg ataacctgcc gcgcctgcgc catccccacg ctacctaagc gccagtgtgg
     2581 ttgccctgcg ctggcgctaa atcccggaat cgctccctgt cagtcaagat tcagcttcag
     2641 ccgctccggg caataaataa tattctgcaa aaccagatcg ttaacggaag cgtaggagtg
     2701 tttatcgtca gcatgaatat aaaagagatc gccacgggta atgcgataag gacgatcgtt
     2761 gagtacatgc agaccattac cgcgccagac aatcaccagc tcacaaaaat catgtgtatg
     2821 ttcggcaaag acatcttgcg gataacggtc agccacagcg actgcctgct ggtcgctggc
     2881 aaaaaaatca tctttgagaa gttttaactg atgcgccacc gtggctacct cggccagaga
     2941 acgaagttga ttattcgcaa tatggcgtac aaatacgttg agaagattcg cgttattgca
     3001 gaaagccatc ccgtccctgg cgaatatcac gcggtgacca gttaaactct cggcgaaaaa
     3061 gcgtcgaaaa gtggttactg tcgctgaatc cacagcgata ggcgatgtca gtaacgctgg
     3121 cctcgctgtg gcgtagcaga tgtcgggctt tcatcagtcg caggcggttc aggtatcgct
     3181 gaggcgtcag tcccgtttgc tgcttaagct gccgatgtag cgtacgcagt gaaagagaaa
     3241 attgatccgc cacggcatcc caattcacct catcggcaaa atggtcctcc agccaggcca
     3301 gaagcaagtt gagacgtgat gcgctgtttt ccaggttctc ctgcaaactg cttttacgca
     3361 gcaagagcag taattgcata aacaagatct cgcgactggc ggtcgagggt aaatcatttt
     3421 ccccttcctg ctgttccatc tgtgcaacca gctgtcgcac ctgctgcaat acgctgtggt
     3481 taacgcgcca gtgagacgga tactgcccat ccagctcttg tggcagcaac tgattcagcc
     3541 cggcgagaaa ctgaaatcga tccggcgagc gatacagcac attggtcaga cacagattat
     3601 cggtatgttc atacagatgc cgatcatgag cgcgtacgaa acagaccgtg ccaccggtga
     3661 tggtataggg ctgcccatta aacacatgaa tacccgtgcc atgttcgaca atcacaattt
     3721 catgaaaatc atgatgatgt tcaggaaaat ccgcctgcgg gagccggggt tctatcgcca
     3781 cggacgcgtt accagacgga aaaaaatcca cactatgtaa tacggtcata ctggcctcct
     3841 gatgtcgtca acacggcgaa atagtaatca cgaggtcagg ttcttacctt aaattttcga
     3901 cggaaaacca cgtaaaaaac gtcgattttt caagatacag cgtgaatttt caggaaatgc
     3961 ggtgagcatc acatcaccac aatgaagcta attgtgaaca tcatcacgtt catctttccc
     4021 tggttgccaa tggcccattt tcctgtcagt aacgagaagg tcacgaattc aggcgctttt
     4081 tagactggtc gtaatgaaat tcagcaggat cacattatga cctttcgcaa ttgtgtcgcc
     4141 gtcgatctcg gcgcatccag tgggcgcgtg atgctggcgc gttacgagcg tgaatgccgc
     4201 agcctgacgc tgcgcgaaat ccatcgtttt aacaatggcc tgcatagcca gaacggttat
     4261 gtcacctgga atgtggatag cctggaaagt gccattcgcc ttggattaaa caaggtgtgc
     4321 gaggaaggga ttcgtatcga tagcattggg attgatacct ggggcgtgga ctttgtgctg
     4381 ctcgaccaac agggtcagcg tgtgggcctg cccgttgctt atcgcgatag ccgctccaat
     4441 ggcctaatgg cgcaggcaca gcaacaactc ggcaaacgcg atatttatca acgtagcggc
     4501 atccagtttc tgcccttcaa tacgctttat caactgcgtg cgctgacgga gcaacaacct
     4561 gaacttattc cacacattgc tcacgctctg ctgatgccgg attacttcag ctatcgcctg
     4621 accggcaaga tgaactggga gtacaccaac gccacgacca cgcaactggt caatatcaat
     4681 agcgacgact gggacgagtc gctactggcg tggagcgggg ctaacaaagc ctggtttggt
     4741 cgcccgacgc atccgggtaa tgtcattggg cactggattt gcccgcaggg taatgagatt
     4801 ccggtggtcg ccgttgccag ccatgatacc gccagcgcgg ttatcgcctc gccgttaaac
     4861 ggttcacgcg ctgcttatct ctcttctggc acctggtcat tgatgggctt cgaaagccag
     4921 acgccattta ccaatgacac ggcgctggca gccaacataa ccaatgaagg cggggcggaa
     4981 ggtcgctatc gggtgctgaa aaatattatg ggcttatggc tgcttcagcg agtgcttcag
     5041 gagcggcaaa tcaacgatct cccggcgctt atcgccgcga cacaggcact tccggcctgc
     5101 cgcttcatca tcaatcccaa tgacgatcgc tttattaatc ctgaggcgat gtgcagcgaa
     5161 attcaggctg cgtgtcggga aatggcgcaa ccgctcccgg aaagtgatgc tgaactggcg
     5221 cgctgcattt tcgacagtct ggcgctgctg tatgccgatg tgttgcatga gctggcgcag
     5281 ctacgcggtg aagatttctc gcaactgcat attgtcggcg gcggctgcca gaacacgctg
     5341 ctcaaccagc tatgtgccga tgcctgcggt attcgggtga tcgccgggcc tgttgaagcc
     5401 tcgacgctcg gcaatatcgg catccagtta atgacgctgg atgaactcaa caatgtggat
     5461 gatttccgtc aggtcgtcag caccaccgcg aatctgacca cctttacccc taatcctgac
     5521 agtgaaattg cccactatgt ggcgcagctt cactcgacac gacagacaaa ggagctttgc
     5581 gcatgaccac tcaactggaa caggcctggg agctggcgaa acagcgtttc gcggcggtgg
     5641 ggattgatgt cgaggaggcg ctgcgccaac ttgatcgttt acccgtttca atgcactgct
     5701 ggcagggcga tgatgtttcc ggttttgaaa acccggaagg ttcgctgacc ggtgggattc
     5761 aggctattgg taattatccg ggcaaagcgc gtaatgccag tgagctacga gccgatctgg
     5821 aacaggctat gcggctgatt ccggggccga aacggcttaa tttacatgcc atctatctgg
     5881 aatcagacac gccagtctcg cgcgaccaga tcaaacctga gcacttcaaa aactgggttg
     5941 aatgggcgaa agccaatcag ctcggtctgg attttaaccc ctcctgtttc tcgcatccgc
     6001 taagcgccga tggctttacg ctctcgcatc ccgacgacag cattcgccag ttctggattg
     6061 atcactgcaa agccagccgt cgcgtttcgg cctattttgg cgagcaactc ggcacaccat
     6121 cggtgatgaa catctggatc ccggatggta tgaaagatat caccgttgac cgtctcgccc
     6181 cgcgtcagcg tctgctggca gcactggatg aggtgatcag cgagaagctg gatccggcgc
     6241 accatatcga cgccgttgag agcaaattgt ttggcattgg cgcagagagc tacacggttg
     6301 gctccaatga gttttacatg gggtatgcca ccagccgcca gactgcgctg tgcctggacg
     6361 ccgggcattt ccacccgact gaagtgattt ccgacaagat ttccgccgcc atgctgtatg
     6421 taccgcagtt gctgctgcac gtcagccgtc cggttcgctg ggacagcgat cacgtggtgc
     6481 tgctggatga tgaaacccag gcgattgcca gtgagattgt tcgtcacgat ctgttcgacc
     6541 gggtgcatat cggcctcgac ttctttgatg cctctatcaa ccgcattgcc gcgtgggtca
     6601 ttggtacacg caatatgaaa aaagccctgc tgcgtgcgtt gctggaacct accgctgagc
     6661 tgcgcaagct ggaagcggcg ggcgattaca ctgcgcgtct ggcgctactg gaagaacaga
     6721 aatcgttgcc gtggcaggcg gtctgggaaa tgtattgcca acgtcacgat acgccagcag
     6781 gtagcgaatg gctggagagc gtgcgggctt atgagaaagc gattttgagc cagcgtgggt
     6841 aaacactgcc ggatgcggcg cgagcgcctt atccggccta caggtcgaat gaccatgcgt
     6901 gctgccaccc tcaccccggc cctctccctg aaagggcgag ggagaaaaac atagcctcgt
     6961 gcggcaacgg agctgaacac cgctccggac agtcccctcg cccctacggg gagagggtta
     7021 gggtgagggg aacaagccgc accatgtcta aacaaggcaa cctaatttac aggaacacag
     7081 aacatgcaaa acattaccca gtcctggttt gtccagggaa tgatcaaagc caccaccgat
     7141 gcctggctga aaggctggga cgagcgcaat ggtggtaacc tgacgctgcg cctggatgac
     7201 gccgatatcg cgccttatca cggcaacttt catgcacaac cgcgctatat tcccctcagc
     7261 caacccatgc ctttactggc aaatacgccg tttattgtca ccggctccgg caaattcttc
     7321 cgtaacgtcc agcttgatcc tgcagctaac ttaggcgtcg taaaagtcga cagcgacggc
     7381 gcgggctacc atattctctg ggggttaacc aacgaagccg ttccaacttc cgaacttccg
     7441 gctcacttcc tttcccactg cgagcgcatt aaagccacca acggtaaaga tcgggtgatc
     7501 atgcactgcc acgccaccaa cctgatcgcc ctcacctatg tacttgaaaa cgacaccgcg
     7561 gtctttactc gccaactgtg ggaaggcagc accgagtgtc tggtggtatt cccggatggc
     7621 gttggcattc tgccgtggat ggtgcccggc acggacgaaa tcggccaggc gaccgcacaa
     7681 gagatgcaaa aacattcgct ggtgttgtgg cccttccacg gcgtcttcgg cagcgggtcg
     7741 acgttggatg aaacattcgg tttaatcgac accgcagaaa aatcagcaca agtattagtg
     7801 aaggtttatt cgatgggcgg catgaaacag accatcagcc gtgaagagtt gatagcgctc
     7861 ggcaagcgct tcggcgttac accactagct agtgcgctgg cgctgtaagg agcaaacatg
     7921 atccgcaaag cctttgtcat gcaggttaac cccgacgccc acgaagagta tcagcgtcgg
     7981 cataacccca tctggccgga actggaagca gtgttgaaat ctcacggtgc gcataactac
     8041 gccatttacc tcgacaaagc gcgtaatctg ctgtttgcca cggtagagat tgaatctgaa
     8101 gaacggtgga atgcggtagc cagcactgat gtttgccaac gttggtggaa atatatgacc
     8161 gatgttatgc ccgctaaccc ggataacagc ccggtgagta gcgagctgca agaagtgttt
     8221 tacctgccgt aattgcccaa tgtgccagag atgcctccgg cacaatattt taaatttcga
     8281 tttgaaaaat taaacatatt acatctcccg gtaagttata tttccctgat acattgtgag
     8341 taaatcacaa aaataatgaa taacccatta atgattcatg tggtttattt aaataaccat
     8401 tatgtgcatt actccgcaaa tctgaccttt cactctgtct caaattgtca ttgtttaccg
     8461 acatatcgcc tctcatcact taatcgctcc accattacaa ttgattaaaa tatattttat
     8521 ggagtccgat taatggcagc tcttactgca agctgtattg acctgaatat tcagggcaat
     8581 ggcgcttatt ccgttctgaa gcagttggcg acaatagcgt tacaaaacgg ttttatcacc
     8641 gactcacacc agttcctgca aaccctgctg ctgcgcgaaa aaatgcactc tactggattt
     8701 ggttccggtg tcgccgtgcc gcacggtaaa agcgcctgcg ttaaacaacc gtttgtatta
     8761 ttcgcccgca aagcgcaggc tattgactgg aaagccagcg atggcgaaga cgtcaattgc
     8821 tggatctgcc tcggcgtgcc gcaaagcggc gaagaggatc aggtcaaaat catcggcaca
     8881 ctgtgtcgca aaattatcca ccaggatttt attcatcaac tgcaacaggg cgacgccgac
     8941 caggtgcttg ccttgttaaa tcaaaccctc agctcataag gaagtggcga tggagtcatc
     9001 cttatgtatt gtcgcgatca ccaactgccc cgccgggatc gctcacacct acatggtggc
     9061 ggaagccctg gaacagaaag cccgttctct cggccatacc ataaaagtgg aaactcaagg
     9121 gtccagtggc gttgaaaacc gcttatccag cgaagagatt gccgctgccg attacgtcat
     9181 tctcgctacc gggcgtggcc tgagcggtga tgatcgcgcg cggtttgccg ggaagaaagt
     9241 ttatgagatt gccatctccc aggcgttgaa aaatatcgac cagattttca gcgaattacc
     9301 gacaaactcg cagctttttg ccgcagatag cggcgtgaag cttggcaaac aggaagtgca
     9361 aagcggcagc gtgatgagcc acctgatggc gggcgtctct gcggcgctgc cgtttgtcat
     9421 cggcggtggt atcctggtgg cgcttgccaa catgctggtg cagttcggtt tgccgtatac
     9481 cgatatgtcg aaaggcgcgc cgtcgtttac ctgggtagtt gaatccatcg gttaccttgg
     9541 cttcaccttt atgatcccca tcatgggcgc ttatattgcc tcgtcgattg ccgataaacc
     9601 ggctttcgct ccggcgtttc tggtttgcta tctggcgaac gacaaagcgc tgctcggcac
     9661 ccagtcgggt gcgggattcc tcggcgcggt ggtgtttctg gtttgctatc tggcgaacga
     9721 caaagcgctg ctcggcaccc agtcgggtgc gggattcctc ggcgcggtgg tgctggggct
     9781 ggcgattggc tatttcgtct tctggttccg caaagtgcgc cttggcaaag cattacaacc
     9841 gctgctcggt tcaatgctga tcccgttcgt tactctgctg gttttcggcg tgctcactta
     9901 ctacgttatc gggccggtga tgtccgacct catgggcggg ctgctccact tcctgaatac
     9961 cattcctcca tcaatgaagt ttgcggcagc gtttctggtc ggtgcgatgc tggcgttcga
    10021 tatgggcggc cccatcaata aaaccgcctg gttcttctgc ttctcactgc tggaaaaaca
    10081 catttatgac tggtacgcca tcgtcggcgt tgtcgcgttg atgccgcccg ttgcggcggg
    10141 tcttgcgacg ttcattgcgc ccaaactgtt tacccgacag gaaaaagagg ctgccagtag
    10201 tgccattgtg gtcggtgcca cagtcgcaac cgaaccggct ataccttacg ctctggcagc
    10261 tccgttgccg atgattaccg ccaatacgct ggcgggtggc attaccggcg tgctggtgat
    10321 cgctttcgga atcaaacgtc tggcacctgg tttgggtatc tttgacccgc tgatcggcct
    10381 gatgtcgccg gtgggttcgt tttatctggt gctagcgatt ggcctcgcgc tgaacatttc
    10441 attcattatc gtcctgaaag gattgtggtt acgccgcaaa gcgaaagccg cgcaacagga
    10501 gcttgtccat gaacattgag ttactgcaac agttgtgcga agccagcgcc gtcagcggcg
    10561 atgaacagga agttcgcgac attctgataa acacgctgga accttgcgtt aatgagatca
    10621 cctttgatgg tctgggcagc tttgttgccc gtaaggggaa taaaggtcca aaagttgccg
    10681 ttgtcaggca tatggatgaa gtcggcttta tggtcaccca catcgacgag agcggttttc
    10741 tgcgctttac caccattggc ggctggtgga atcagtcaat gctcaaccac cgggtaacca
    10801 tacgcacaca caagggattt aaaatccctg gtgtgattgg ttccgtcgca cctcatgcgt
    10861 taacggaaaa gcaaaagcaa cagccgctgt catttgatga gatgttcatt gatattggcg
    10921 cgaacagtcg cgatgaggtg gaaaaacgcg gtgtggcgat gggcgatttt attagcccgg
    10981 aagccaattt tgcctgctgg ggcgaagata aagtggtcgg caaggcgctg gataaccgca
    11041 tcggctgcgc aatgatggct gaactattgc agacggtgaa taatcccgaa attacgctgt
    11101 atggcgtcgg cagcgtggaa gaagaagttg ggctacgcgg ggcacaaacc tcggcggaac
    11161 acattaaacc ggatgtggtg attgtgctgg ataccgctgt cgcaggtgat gttccgggca
    11221 ttgataacat taaatacccg ctgaaactgg gccaggggcc ggggctgatg ctgtttgaca
    11281 agcgctactt ccccaaccag aaactggtgg cggcgttaaa aagctgtgcc gcacataacg
    11341 atttaccgct gcaattttcc accatgaaaa ccggtgcgac ggatggcggg cgctacaacg
    11401 tgatgggcgg cgggcgtccg gttgttgcgc tgtgcctgcc aacccgctat ctgcacgcta
    11461 acagcggcat gatttcaaaa gccgattacg atgctctgct cacgttgata cgggattttc
    11521 tgaccacctt aaccgcggag aaagtcaacg cgtttagcca gttccgtcag gtggattaat
    11581 gctaaacgaa cgccagttaa agattgtcga tctgctggag caacagccgc gcacgcctgg
    11641 tgagctggcg caacagactg gcgtttcagg caggaccatc ctgcgtgata ttgactatct
    11701 caactttacc cttaacggca aagcccgcat tttcgccagt ggcagtgcgg gctatcagct
    11761 ggaaatcttc gagcgccgca gcttttttca gttgctgcaa aagcacgata acgacgatcg
    11821 gctgctggcg ctgttattac tgaatacctt tactccccgt gcgcaactcg cctcggcgct
    11881 taatttgcca gaaacctggg tagcagagcg tctgccccgg ttaaaacagc gttatgaacg
    11941 cacttgttgc ctggccagtc gccccggttt gggccatttc attgatgaga cagaagagaa
    12001 acgcgttatc ttgctggcga acttgctgcg caaagatccg tttttaattc cgctggcggg
    12061 cgtaacacga gacaattttc agcatttatc cacggcctgc gacaaccaac accgctggcc
    12121 gctcatgcag ggtgattatc tctccagcct gattctggcg atttacgccc tgcgtaatca
    12181 actgaccgat gagtggccgc aatatcccgg taacgagata aaacaaatcg ttgaacagag
    12241 cggcatgttt cttggtgata acgctgtaag aaccctgaca ggtttgatag agaaacagca
    12301 tcagcaagcg caggtaattt cagccgatca tgtgctgggg ttgctgcaaa gggtgccagg
    12361 catcgcgtca ttgaatatta ttgatacgca gctggttgag aatattaccg ggcatttatt
    12421 acgttgcctt gccgcaccgg tgtggattgc cgagcaccgc caaagcagca tgaataacct
    12481 gaaagccgcc tggcctgcgg cctttgatat gagtctgcac tttattacgc tactgcgtga
    12541 acagctcgat attccccttt tcgatagcga tctgatcggt ttgtattttg cctgtgcgct
    12601 ggaacggcat caaaacgaac gccagccgat cattttgctc tcggaccaga acgcgattgc
    12661 cactattaat cagctcgcca ttgagcgcga tgttttacat tgtcgggtga ttattgcccg
    12721 tagcttaagc gagcttattg ccattcgcga agagattgag ccgttattga tcattaacaa
    12781 cagccattat ttactggatg acgcggtaaa taattacatc accgtaaaaa atatcattac
    12841 ggctgccgat atcgaacaaa taaaacactt tctggcgacg gcatttattc gccaacagcc
    12901 ggaacgcttt ttctctgccc ccggaagttt tcattattcg aatgtacgcg gtgaaagctg
    12961 gcaacatatt acccggcaaa tttgtgcgca attagtggca caacaccata ttaccgccga
    13021 tgaagcgcaa cggatcatcg cccgcgaagg cgaaggtgaa aacctgattg ttaatcgcct
    13081 cgccatccca cattgctgga gcgaacagga gcgacgtttt cgtggatttt ttattaccct
    13141 cgcccaacca gttgaggtga ataacgaagt cattaaccat gtcttgatcg cctgcgccgc
    13201 cgccgatgcg cgtcatgagc taaaaatatt tagctatctg gcaagcgtat tgtgtcagca
    13261 tccggcagaa gttattgccg ggttaacagg atatgaggca tttatggagt tac
//
LOCUS       DAZPAC010000109        13290 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZPAC010000109 DAZPAC010000000
VERSION     DAZPAC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13290
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.109"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(477..1568)
                     /gene="ychF"
                     /locus_tag="ACHUPM_002619"
     CDS             complement(477..1568)
                     /gene="ychF"
                     /locus_tag="ACHUPM_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR0870354.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1685..2269)
                     /gene="pth"
                     /locus_tag="ACHUPM_002620"
     CDS             complement(1685..2269)
                     /gene="pth"
                     /locus_tag="ACHUPM_002620"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR0870355.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2546..2824
                     /gene="ychH"
                     /locus_tag="ACHUPM_002621"
     CDS             2546..2824
                     /gene="ychH"
                     /locus_tag="ACHUPM_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR0870356.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2879..4558)
                     /gene="dauA"
                     /locus_tag="ACHUPM_002622"
     CDS             complement(2879..4558)
                     /gene="dauA"
                     /locus_tag="ACHUPM_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR0870357.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4682..5629)
                     /gene="prs"
                     /locus_tag="ACHUPM_002623"
     CDS             complement(4682..5629)
                     /gene="prs"
                     /locus_tag="ACHUPM_002623"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR0870358.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5780..6631)
                     /gene="ispE"
                     /locus_tag="ACHUPM_002624"
     CDS             complement(5780..6631)
                     /gene="ispE"
                     /locus_tag="ACHUPM_002624"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR0870359.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6631..7254)
                     /gene="lolB"
                     /locus_tag="ACHUPM_002625"
     CDS             complement(6631..7254)
                     /gene="lolB"
                     /locus_tag="ACHUPM_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR0870360.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7468..8724
                     /gene="hemA"
                     /locus_tag="ACHUPM_002626"
     CDS             7468..8724
                     /gene="hemA"
                     /locus_tag="ACHUPM_002626"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR0870361.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8766..9848
                     /gene="prfA"
                     /locus_tag="ACHUPM_002627"
     CDS             8766..9848
                     /gene="prfA"
                     /locus_tag="ACHUPM_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR0870362.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9848..10681
                     /gene="prmC"
                     /locus_tag="ACHUPM_002628"
     CDS             9848..10681
                     /gene="prmC"
                     /locus_tag="ACHUPM_002628"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0870363.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10678..11070
                     /gene="sirB2"
                     /locus_tag="ACHUPM_002629"
     CDS             10678..11070
                     /gene="sirB2"
                     /locus_tag="ACHUPM_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR0870364.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11074..11882
                     /gene="sirB1"
                     /locus_tag="ACHUPM_002630"
                     /pseudo
     CDS             11074..11882
                     /gene="sirB1"
                     /locus_tag="ACHUPM_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
     gene            11918..12772
                     /gene="kdsA"
                     /locus_tag="ACHUPM_002631"
     CDS             11918..12772
                     /gene="kdsA"
                     /locus_tag="ACHUPM_002631"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR0870365.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12921..13028)
                     /locus_tag="ACHUPM_002632"
     CDS             complement(12921..13028)
                     /locus_tag="ACHUPM_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0870366.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3307 a         3345 c         3431 g         3207 t
ORIGIN      
        1 aatatgcgcc atttttgcat tactaataag aaaaagcaaa aaacatcgat attcattttt
       61 ggtcaatggg ttttctggcc tatcgttgtt attgtgctac tggttatcat tttcatcttg
      121 ccaggatcgc acgtaatcac gttattcggg gactttatct gcgggatatt acccttcagg
      181 taagtccttt tctcgccagc atattggagc gtgatcaata ttgatcagct gtgaaggggg
      241 tgatgtgtag ttgaacaaaa acttacgtta tatcactact aatgagatat ccgtcatctg
      301 acactgatgt ctttaattat tctcgtggtt cactgtaggc aataagcaca aaagtgtagg
      361 atgttacaag aatgattagg actcggtgaa atgaaaaatc cacgcaattg cgtggatttt
      421 atatactttt gcgctcttca tgagatttag cgaaacctca tgagacaata aattaattag
      481 acgttgaaaa ggaagttcat cacatcgcca tctttaacga tgtaatcttt accttctgca
      541 cgcattttgc ctgcttcttt cgcgccttgt tcacctttgt aagtgatgaa atcttcaaac
      601 gagatggttt gtgcacggat aaagcctttt tcaaaatcag tatggatttt gcccgctgcc
      661 tgcggcgcgg ttgctccaac cggaatggtc catgcacgca cttctttcac cccagcggtg
      721 aagtaagttt gcaggttcag cagcttataa ccgacacgga tcacacggtt caggcccggt
      781 tcttccagcc caagctcctg cataaactcg tcacgttctt cgtcgtccag ttcggcaatg
      841 tctgcttcaa cagcagcaca aaccggaacc acaacagaac cttctttcgc cgcgatttca
      901 cgcacctggt caagatatgg gttgttttca aaaccgtctt cgttgacgtt ggcgatgtac
      961 attgttggtt tcagcgtcag gaagctcagg taacgaatag ccgctttctc ttcagcgctt
     1021 aaatccagcg cgcgcagcat acctgcgttt tccagctggg gcaggcattt ttctaggacc
     1081 gccagctcag ctttcgcgtc tttatcgcca cctttggctt tcttctgtac gcgatgaatc
     1141 gcacgttcgc aggtgtcgag gtctgccagc gccagttcgg tgttgataac ttcaatatcg
     1201 tcagccgggt taactttgcc ggaaacgtga atgatgttgt cattttcaaa gcagcgaaca
     1261 acgtgaccga tcgcttcggt ttcacggatg ttggtcagga actggttacc cagaccttcg
     1321 cctttcgatg cgccttttac cagaccggcg atatcgacaa attccatggt cgtgggaagc
     1381 gtacgttgcg gttttacgat ttcagccagt tgatccaggc gagggtcagg cattggtacg
     1441 acgcctgtgt tcggctcaat ggtgcagaat ggaaagttgg ccgcttcaat accggctttg
     1501 gtcagcgcgt tgaacagggt agatttcccg acgttgggca aaccgacgat accgcatttg
     1561 aatcccatga tttaaatcac cttaatatct taataatcaa cctgttattg ctaacagatt
     1621 gcagaaatgg aaataacttt gcctattata cacggcactc ggcaaaaatg ccgcagacaa
     1681 cgacttattg cgctttaaag gcgtgcaatc ggttcgttgc tttggtcaag ccatctgtaa
     1741 accacatttc agtacaacgc gccgcttcgt cgatggcttc atcaattaac ttctgttcac
     1801 taacaggcgg tttgcctaac acaaaaccga caactttatt tttatcgcca ggatgaccga
     1861 ttccgatgcg taaacggtga aagttagggt tattacccaa tttactgatg atgtctttca
     1921 gtccattgtg accaccatgg ccaccgccca atttaaattt ggcgacgcca ggaggcagat
     1981 ccagttcgtc gtgggccacc agaatttcgt ccggattaat gcggaaaaaa ctggccatcg
     2041 ccgcaacggc tttgccgctg agattcataa atgtagtcgg gactaacagg cggacatctt
     2101 cgcctccaag agtgactcgc gaagtataac caaagaattt agcctcttcg cgcagcggag
     2161 cgcgcaaacg ctctgccagt aagtcaacga accaggcacc agcattatgt cgcgttgcgg
     2221 cgtattcagc accggggttc gccaggccga caatcaattt aatcgtcacg ttttttgtcc
     2281 tgagtgtgta cataactggc gcgtagttta ctggttgcgg ccccgcttga caaaaaactg
     2341 cgtatcaaat gcagataacg taataattgc ctgagtggac tattagaaag tcaaggtgtt
     2401 caggcgttta tttgtaaagt tttgttgaaa taagggttgt aattgtgatc acgcccgcac
     2461 ataacccaca gggtgttgtc tatactttac acataaggaa gaggggtatt ccctgttaca
     2521 acccagaaag ttccggaggt gacatatgaa acgcaaaaac gcttcgttac tcggtaacgt
     2581 gctcatgggg ttggggctgg tggtaatggt ggtcggcgtg gggtattcaa tcctcaacca
     2641 gttaccacag tttaatatgc cccagtattt cgcacatggt gcagtgctaa gtattttcgt
     2701 cggtgccatt ctctggctgg cgggtgcccg tgttggcggg catgaacagg tgtgcgaccg
     2761 ttactggtgg gttcgtcact atgacaaacg ttgccgccgt agcgataatc gccgtcatag
     2821 ctaacaaata atgcagtttg ctgaccagtc agtttgcgct gactggtcaa tctcgtactt
     2881 ataaatccgc catcgccgcg cgacgattcg ggaagaacgc gaggcgtccc gggatcggtt
     2941 gaatgcctgc gcgagccata gtgcgcagtg gctggaattc cacgttgcac acgcgcagtt
     3001 cacatccttc gggcagacgc ttcacaaaac gctggaacgc atcaagacca ccagcatcaa
     3061 gtaccggaac ggcatcccac ttcagaatca caatccgttt gccttcaaga cgtgactcca
     3121 ggtccgtgaa caagccttca gcagcagcaa aaaacagcgg gccaataacg cgcagtacca
     3181 gaacatcgtc tggaacatct acgactaccg gtgccaggcg agtcatacgt gcgatacgac
     3241 gcataaacag cagcgatgcc agcacgatcc ccacgctgat ggcaataacc atatcaaaca
     3301 gcacggtcag cgacatgcac agcagcatga caatgatgtc atctttcggt gcatgacgca
     3361 gcaagtcgac cactttatgc gcttcactca tgttccacgc caccatcaac agcagggctg
     3421 ccatagcgga aagcggcagc caggagagca gcggtgccag taccagcagg gcaagaataa
     3481 ccagaataga gtggatcacc gccgagatag gggaagttgc cccggcacgg acgttagcgg
     3541 cagaacgcgc gatggcagct gtagcggtaa taccaccaaa gaacggagcg ataatattcc
     3601 ccagtccctg tccaaccagt tcgctgttcg ccttgtgttt cgtcccggtc ataccatcca
     3661 gtaccacggc gcagagcaga gattcgattg cgccgagcat tgccattgag aatgccgcag
     3721 gcagcagtgt gcgaatagaa tcccaggtta gcgtgaattc tgaattaggc agatcccacg
     3781 gcagcaccag ttgcggcagc agttgcggaa taccgttacc ctgagaacca tcggccagga
     3841 cgtagtggaa ttgcgaaccg atggtagcaa catgtccgcc gagcaggtta acaatcccca
     3901 tcaccgcgca accagccagc aaggccggaa ggtgaccggg taaacgaatg cccagacgcg
     3961 gccaaaaaac aagaatacct agcgtcacaa tgccaatggc agcatcaccc acattaatgg
     4021 tcggcagcgc cataaataat gcgccgactt tttgtagata atgttccggg acatgggcca
     4081 tttgcagacc gagaaaatct ttaatctgca tggtaccgat ggtgatcccg atacccgagg
     4141 tgaaacctaa ggtgacggaa accggaatat actcaatcag gcgaccaaag cgtgccagac
     4201 ccatcagaat caaaaagatc cccgacagca aggtcgcaac cagcagtcct gccagtccaa
     4261 actgttgcga cacgggatag agaattacca caaatgccgc agtcggaccg gaaacgctaa
     4321 agcgtgaccc acccgtcaga gcaatgacaa tccccgcaac agctgcggta tataaaccgt
     4381 actggggtgc cacaccacta ccaatagcca acgccatcgc cagcgggata gcaataatcc
     4441 cgacggttat cccggcaatc aggtcacggg taaaccgtgc ggcagtatat ttttctttcc
     4501 agcaagcgtc gatcagagcg cggaaaggca tcacatgtga ggaaaatatt ttgttcacaa
     4561 taatgtttca tccgtgagcg catcatctgt caactaaatg gcaggtgaag gaggcatagg
     4621 tcatacaaat ggatattaca gacaaaaaaa cccgccgcag cgggtctttg agcgggttcg
     4681 attagtgttc gaacatggca gagatcgatt cttcgttgct gatacgacga atcgcttcgg
     4741 ccagcatacc tgacagggtc agagtacgca cgttcggcag tgatttgatt tcatcgctca
     4801 gcggaatggt atcgcagaca acgacttcat caattacaga gttacgcagg ttgttcgccg
     4861 cgttgccaga gaagatcggg tgagtcgcgt acgcaaatac acgtttagca ccacgttctt
     4921 tcagagcttc agcagcttta cacagcgtac cgccagtgtc gatcatatca tcgaccagta
     4981 cgcagtcacg acctgcaacg tcaccgatga tatgcatcac ctgggaaacg ttcgcacgcg
     5041 gacgacgttt gtcgatgatt gccatatcgg tatcgttcag cagcttagcg atagcgcggg
     5101 cacgcacaac gccgccgatg tccggagaaa ccacaattgg gttatccaga ttcagctgca
     5161 gcatgtcttc cagcaggatc gggctaccaa atacgttatc aaccggaacg tcgaagaaac
     5221 cctgaatctg ttcagcgtgc agatccactg tcagcacacg gtcaacaccg acgctggaga
     5281 ggaagtctgc aaccactttc gcagtgattg gtacacgagc ggaacggacg cgacggtcct
     5341 ggcgcgcata gccaaagtag gggataacag cggtgatacg acctgcggaa gcacgacgca
     5401 gggcatcaac cataacgact aattccatca ggttgtcgtt agtaggggca caagtggact
     5461 ggatgatgaa aatatcacca ccgcgtacat tttcgttgat ttgtacactg acttcgccgt
     5521 cgctaaagcg acctacagcg gcgtcgccga gtgaagtgta caggcggttg gcaatacgtt
     5581 gtgctagttc cggggtggcg ttaccagcaa aaagcttcat atcaggcacg agaagaacct
     5641 caggcatgcg tccattggtg gaaagaatct gccgaaaact gtgcgggcca ggcatgatcc
     5701 tttccaggcg gtgtattaaa gagcgcgatg caacgtctgg aacaaggtga cgttgtcacc
     5761 gaaactcagc ttgcccggct taaagcatgg ctctgtgcaa tggggaaaga ttaacacctt
     5821 tcgccacaaa gccattgagc cattccgggg cttgctctag cacctggcgg gcttcagact
     5881 ctgtatcaaa ttcagcaaag acacaggccc ctgtcccagt caggcgcgac ggggcgtatt
     5941 ctaacagcca ggaaagcacc gcatcaacct cgcgaaaacg ttttcttgcg ataacctcgc
     6001 aatcattgct gaattcacat tttagcaacg tttctattga cctttttggc gtattgcgcg
     6061 ggagttcagg atctttaaaa atcactggtg tcggaatact tacaccaggg tgcgccacca
     6121 gataccactt ctctggcgga tccaccggcg ttagtatttc accaacgcct tcggcaaacg
     6181 cggcatgccc ccgaacaaag acaggaacat ctgcgcccag cgtcagcccc atttccgcca
     6241 gctcatccat gcttagcccg cattgccaga gatgatttaa tgccaccagg accgtcgcgg
     6301 cattggatga accaccgccg agaccgccgc ccatcggcaa acgcttgtca atgctgatat
     6361 tcgcaccgct tcccgtcgga agacgcccgc tgtctgccgc agttttcatc aacaaccgcg
     6421 ctgcgcgaac gatcaggtta tcttcatgtt ccacgccttc aacgggcgtt aacagacgaa
     6481 tatccccatc gtcacgaagc tcaatgctga tggtgtcgcc gtaatcaaga aactgaaaca
     6541 gcgtttgcag cgtgtggtaa ccatccgcac gctgaccggt aatgtataaa aacagattaa
     6601 gttttgccgg agagggccac tgtgtccgca ttatttcact atccagttat ccatttttaa
     6661 cttgatgcgt tgaccaccgt cggtgagttc catattggct ggcatcgcag gttgcgtttt
     6721 ggtgtcataa ccaccataaa caaccttcca gtttttgcca ttctggctgt aggtaatttc
     6781 gctcaggcgg tactggtcgt ccagtttgta gtcggttgca tcacccggta aacctaaaat
     6841 ccattggcgc aagctgttga gcggaattgg cattccggtc aatttgccaa tcatctcttc
     6901 ggcgtcatcg gcggtataac gctgaccttt attgtcgact aactgcacgt tacccggttg
     6961 agcattcagc tccagttccg tgctgcccaa tgggttagtg agcagcagac ggtagcgatc
     7021 ctggccggtt tgctgccaga aaaagcgggc gtacactttt tgttggtcag aaatataagc
     7081 gaacgcgccg cgagtctgat actgattaag attgcgcacg tcttgctgat gctgacgcca
     7141 ttgtggcgaa tccgggcttt tgccaggacc tttgggcgtg gtaacggaac aggcagtgag
     7201 cacaagagca gccagcggta gcaggcggat aagacgaaaa tcgggctggg gcatagtgat
     7261 gacaagtcct tgagatacgt tgcagttata acccttaatg ctagcgttac cgtccgctat
     7321 cgtctatgtt caagttgtct taattgccag aatctaacgg ctttcggcaa ttactccaaa
     7381 agggggcgct ctcttttatt gatcttacgc atcctgtatg atgcaagcag actaaccata
     7441 tcaacgttgg tattatttcc cgcagacatg acccttttag cactcggtat caaccataaa
     7501 acggcacctg tatcgctgcg agaacgtgta tcgttttcgc cggataagct cgatcaggcg
     7561 cttgacagcc tgcttgcgca gccgatggtg cagggcggcg tggtgctgtc gacgtgcaac
     7621 cgcacggaac tttatcttag cgttgaagag caggataacc tgcaagaggc gttaatccgc
     7681 tggctttgcg attatcacaa tcttaatgaa gaagatctgc gtaaaagcct ctactggcat
     7741 caggataacg acgcggttag ccatttaatg cgtgttgcca gcggcctgga ttcactggtt
     7801 cttggggagc cgcagatcct cggtcaggtt aaaaaagcgt ttgccgattc gcaaaaaggt
     7861 catatgaagg ccagcgaact ggaacgcatg ttccagaaat ctttctctgt agcgaaacgc
     7921 gttcgcactg aaacagatat cggtgccagt gctgtgtctg tcgcttttgc ggcttgtacg
     7981 ctggcgcggc agatctttga atcgctctct acggtcacag tgttgctggt aggcgcgggc
     8041 gaaaccatcg agctggtagc gcgtcatctg cgcgaacata aagtacagaa gatgattatc
     8101 gccaaccgca ctcgcgaacg tgcccaaatt ctggcagatg aagttggcgc ggaagtgatt
     8161 gccctgagtg agatcgacga acgtctgcgc gaagccgata tcatcatcag ttccaccgcc
     8221 agcccgttac cgattatcgg gaaaggcatg gtggagcgcg cattaaaaag tcgtcgtaac
     8281 caaccaatgc tgttggtgga tattgccgtt ccgcgcgatg ttgagccgga agttggcaaa
     8341 ctggcgaatg cttatcttta tagtgtggac gatctgcaaa gcatcatttc gcacaacctg
     8401 gcgcagcgta aagccgcagc ggttgaggcg gaaactattg tcgctcagga aaccagcgaa
     8461 tttatggcgt ggctgcgagc acaaagcgcc agcgaaacca ttcgcgagta tcgcagccag
     8521 gcagagcacg ttcgcgatga gttaaccgcc aaagcgttag cggcccttga gcagggcggc
     8581 gacgcgcaag ccattatgca ggatctggca tggaaactga ctaaccgctt gatccatgcg
     8641 ccaacgaaat cacttcaaca ggccgcccgt gacggggata acgaacgcct gaatattctg
     8701 cgcgacagcc tcgggctgga gtagcagtac atcattttct ttttttacag ggtgcattta
     8761 cgcctatgaa gccttctatc gttgccaaac tggaagccct gcatgaacgc catgaagaag
     8821 ttcaggcgtt gctgggtgac gcgcaaacta tcgccgacca ggaacgtttt cgcgcattat
     8881 cacgcgaata tgcgcagtta agtgatgttt cgcgctgttt taccgactgg caacaggttc
     8941 aggaagatat cgaaaccgca cagatgatgc tcgatgatcc tgaaatgcgt gagatggcgc
     9001 aggatgaact gcgcgaagct aaagaaaaaa gcgagcaact ggaacagcaa ttacaggttc
     9061 tgttactgcc aaaagatcct gatgacgaac gtaacgcctt cctcgaagtc cgagccggaa
     9121 ccggcggcga cgaagcggcg ctgttcgcgg gcgatctgtt ccgtatgtac agccgttatg
     9181 ccgaagcccg ccgctggcgg gtagaaatca tgagcgccag cgagggtgaa catggtggtt
     9241 ataaagagat catcgccaaa attagcggtg atggtgtgta tggtcgtctg aaatttgaat
     9301 ccggcggtca tcgcgtgcag cgtgttcctg ctacggaatc gcagggtcgt attcatactt
     9361 ctgcttgtac cgttgcggta atgccagaac tgcctgacgc agaactgccg gacatcaacc
     9421 cagcagattt acgcattgat actttccgct cgtcaggggc gggtggtcag cacgttaaca
     9481 ccaccgattc ggcaattcgt attactcact tgccgaccgg gattgttgtt gaatgtcagg
     9541 acgaacgttc acaacataaa aacaaagcta aagcactttc tgtacttggt gctcgtatcc
     9601 acgctgcaga aatggcaaaa cggcaacagg ccgaagcgtc tacccgtcgt aacctgctgg
     9661 ggagtggcga tcgcagcgac cgtaaccgta cttacaactt cccgcagggg cgcgttaccg
     9721 atcaccgcat caacctgacg ctctaccgtc tggatgaagt gatggaaggt aagctggata
     9781 tgctgattga accgattatc caggaacatc aggccgacca actggcggcg ttgtccgagc
     9841 aggaataatg gaatatcaat actggttacg tgaagcaata agccaacttc aggcgagcga
     9901 aagcccacgc cgtgacgctg aaatcttatt ggagtatgtc actggcaggg ggcggacttt
     9961 tattctggcc tttggtgaaa cgcagctgac tgacgaacaa tgtcagcaac ttgatgcgct
    10021 actgacacgt cgtcgcgatg gtgaacccat tgctcattta accggggtgc gagaattctg
    10081 gtcgttgccg ttatttgttt cgccagcgac cttaattccg cgcccggata cggagtgtct
    10141 ggtggagcag gcactggcgc ggttgcctga acaaccttgc cgtattctcg atctcgggac
    10201 gggtaccggg gcgattgcgc ttgcgctggc tagcgagcgc ccggactgcg aaattatcgc
    10261 tgtagatcgt atgcctgatg ctgtctccct ggcacaacgt aatgcccagc atctggcgat
    10321 caaaaatatc cacattctgc aaagcgactg gtttagcgcg ctagccgggc agcagtttgc
    10381 gatgattgtc agcaatccgc cgtatattga cgagcaggac ccacatcttc aacaaggcga
    10441 tgtccgcttt gagccgctca ctgcgctggt tgcggcagac agtggaatgg cagacatcgt
    10501 gcatatcatc gaacagtcgc gtaacgcgct ggtatccggc ggctttctgc ttctggaaca
    10561 tggctggcag cagggcgaag cggtgcgaca agcatttatc ctcgcggggt atcatgacgt
    10621 cgaaacctgc cgtgactatg gtgataacga gcgcgtaacg ctcggccgct attatcaatg
    10681 acaagttttt ctacactgct tagtgttcat cttattagta tcgcgctttc tgttgggcta
    10741 ttaaccttac gtttctggct acgttatcag aagcatccac aggcatttgc tcgctggacg
    10801 cgcattgtgc cgccggttgt cgatacggtg ttattgttaa gcggcattgc gttgatggct
    10861 aaagcgcaca tcctgccatt ttccgggcag gcacagtggc tgactgaaaa gctgtttgga
    10921 gttatcattt atatcgtttt gggttttatt gcactcgatt atcgtcgtat gcacagtcag
    10981 caggcgcgca ttattgcctt cccgctggcg ttggtggtgc tgtacatcat cattaaactc
    11041 gccaccacaa aagtaccgtt actggggtaa gtcatgagat cgttagctga tttcgaattt
    11101 aataaagcgc cattgtgcga aggcatgatc ctggcttgcg aagcaatccg ccgcgatttt
    11161 ccctcgcaag atgtttacga cgaactggag cgtctcgtta gtctggcgaa ggaagaaatc
    11221 agccagcttc tgcctttaga agagcagctg gaaaaactga tcgcgctgtt ttacggcgac
    11281 tggggattta aagcctcacg cggcgtttat cgtctttccg atgcattatg gctggaccag
    11341 gtgttaaaga atcgacaggc agtgcggtat cattaggtgc ggttttatta tgggtcgcga
    11401 atcgtctcga tttgccgctg ctgccggtga ttttccctac gcagctgata ttgcgcattg
    11461 aatgtccgga tggcgaaatt tggctgatta atccttttaa cggtgaatcg ttaagcgaac
    11521 atatgctgga cgtatggtta aagggaaata tcagcccgtc ggcggaactg ttttatgaag
    11581 accttgatga agctgataac attgaggtaa tccgcaaatt gttggataca ctcaaagcct
    11641 cgttgatgga agaaaatcag atggagctgg cgttacgcac cagcgaagct ttattacaat
    11701 tcaacccgga agatccctat gaaattcgcg atcgcgggtt gatttatgcg caactggatt
    11761 gcgaacacgt tgcgttgaac gatttaagtt atttcgttga acagtgtccg gaagacccga
    11821 tcagcgaaat gatccgtgcg caaataaata acatcgcgca taaacatatt gtgctgcatt
    11881 aattaatcga cattttactc aagattaagg cgatcctatg aaacaaaaag tggttagcat
    11941 tggcgacatc aacgtagcaa atgacctgcc gttcgtactg tttggtggta tgaacgtgct
    12001 ggaatctcgc gatctggcga tgcgcatttg cgagcactac gtaaccgtga cccagaaact
    12061 gggtatccct tacgtgttca aagcctcttt tgacaaagcc aaccgctcct ccatccactc
    12121 ttatcgtgga ccgggcctgg aagaagggat gaaaatcttc caggagttga agcagacttt
    12181 tggcgtgaaa attatcaccg acgttcacga accaagtcag gcacagcccg ttgctgatgt
    12241 cgtggatgtg attcagttgc cggcgtttct tgctcgccag actgacctgg ttgaagccat
    12301 ggcgaaaacc ggtgcggtaa ttaacgtcaa gaaaccacag tttgtcagcc cgggacagat
    12361 gggtaatatc gttgataaat tcaaagaagg cggcaacgaa aaagtgattc tttgcgatcg
    12421 cggtgctaac ttcggctatg acaacctggt tgtcgatatg ctgggcttca gcattatgaa
    12481 gaaagtgtct ggtaactcgc cggtgatttt cgacgtgacc cacgcactgc aatgccgcga
    12541 tccgtttggc gcagcttccg gtggtcgtcg tgctcaggtg gctgagctgg cacgagccgg
    12601 tatggcggta ggtctggcgg ggctgtttat tgaagcgcat ccggatccgg aacatgcgaa
    12661 atgtgatggt ccatccgcgc tgccgctggc taaactggaa ccgttcctca agcagatgaa
    12721 agcgattgat gatctggtga aaggtttcga agaactggat accagcaagt aatctttttt
    12781 gcttgaaaaa taaagcctta gcgttctgcg ttaagacttt tttcatgggt gccagataca
    12841 aaaaaggccg caggctgtta cccctgcggc cggtttcggg cgcatattgc catcacggca
    12901 gcctgacgcc cgttttcacc ttacttccgg ttacgccacc agccgacaat cgctgcggta
    12961 ataattcccg ccaggatcgg tgctgccagg tcgtgccaga aaatcatggc aaactgcgcg
    13021 agcgtcatat agccgccttg ttgtaatgac aacattttgc ggctattctt gaattgttct
    13081 ggttcaagat tagcccccgt tctgttgtca ggttaaacct ctcaacgtgc gggggttttc
    13141 tctttccagc aaccaatgcc accagggata aagcccccgc aacattgcgc ctcaccggat
    13201 aacgccggct tgatgtggat actacgtctc aattcatctt cacttcatcc ctgaaatgtt
    13261 tgcaatgaag agtgcattcc ggtttttcaa
//
LOCUS       DAZPAC010000110        13260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZPAC010000110 DAZPAC010000000
VERSION     DAZPAC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.110"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="ACHUPM_002633"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="ACHUPM_002633"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR0870367.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHUPM_002634"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHUPM_002634"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR0870368.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHUPM_002635"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHUPM_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR0870369.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="ACHUPM_002636"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="ACHUPM_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR0870370.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="ACHUPM_002637"
     CDS             complement(4036..5568)
                     /locus_tag="ACHUPM_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR0870371.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACHGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="ACHUPM_002638"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="ACHUPM_002638"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR0870372.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="ACHUPM_002639"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="ACHUPM_002639"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR0870373.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="ACHUPM_002640"
     CDS             complement(8663..10399)
                     /locus_tag="ACHUPM_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR0870374.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHUPM_002641"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHUPM_002641"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR0870375.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="ACHUPM_002642"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="ACHUPM_002642"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR0870376.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="ACHUPM_002643"
     CDS             complement(12121..12855)
                     /locus_tag="ACHUPM_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR0870377.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13260
                     /locus_tag="ACHUPM_002644"
     CDS             13056..>13260
                     /locus_tag="ACHUPM_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR0870378.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3365 a         3370 c         3489 g         3036 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatggcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc tgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
//
LOCUS       DAZPAC010000111        13229 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZPAC010000111 DAZPAC010000000
VERSION     DAZPAC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13229
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.111"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..696)
                     /locus_tag="ACHUPM_002645"
     CDS             complement(<1..696)
                     /locus_tag="ACHUPM_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0870379.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="ACHUPM_002646"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="ACHUPM_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR0870380.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHUPM_002647"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHUPM_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR0870381.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="ACHUPM_002648"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="ACHUPM_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR0870382.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="ACHUPM_002649"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="ACHUPM_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR0870383.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="ACHUPM_002650"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="ACHUPM_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR0870384.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="ACHUPM_002651"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="ACHUPM_002651"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR0870385.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="ACHUPM_002652"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="ACHUPM_002652"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR0870386.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="ACHUPM_002653"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="ACHUPM_002653"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR0870387.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="ACHUPM_002654"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="ACHUPM_002654"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR0870388.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="ACHUPM_002655"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="ACHUPM_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR0870389.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHUPM_002656"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHUPM_002656"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR0870390.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /gene="acpS"
                     /locus_tag="ACHUPM_002657"
     CDS             10119..10499
                     /gene="acpS"
                     /locus_tag="ACHUPM_002657"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR0870391.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="ACHUPM_002658"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="ACHUPM_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR0870392.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="ACHUPM_002659"
     CDS             complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="ACHUPM_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR0870393.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11470..12660
                     /locus_tag="ACHUPM_002660"
                     /pseudo
     CDS             11470..12660
                     /locus_tag="ACHUPM_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12845..>13229)
                     /locus_tag="ACHUPM_002661"
     CDS             complement(12845..>13229)
                     /locus_tag="ACHUPM_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0870394.1"
                     /translation="GQDEIGLRYNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVR
                     YYGFLSPAKRRLLEEVVYIITETVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFT
                     GLKRGYRLAEQVLMHELLARMRWCG"
BASE COUNT         3357 a         3271 c         3418 g         3183 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggctc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccatg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagctt ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 gctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcgagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataact
    10861 ataaataagt gtcttggcag atactatata aattaactta gtgaatgatt atgctaatgt
    10921 catcaattaa ataaatataa tggcgttaag gcttcccagt aatataatta atactctact
    10981 tccagagtag aatattaaat tttatccgcg tggtgcatca gcacaaattt atcccacaac
    11041 tgttcttctg tctcgacatg cgccggatct ttcacaatag tattggggat cgggcacacc
    11101 ttctggcagg ttggtgtctc gtagtgccct acgcattcgg tacacttatc gctgttaatc
    11161 tcgtagatat gatctcccat tgaaatcgcc tcattcgggc attcgggttc acacatatca
    11221 caattaatac agcgtttagt aattagtaaa gacatttcaa tggattaccg ttaaatcatt
    11281 ttaaaatcag taagttgtat cgagtttgta tgctttactg tcattaactt actgtatgtt
    11341 gatccagtgt atttaacctt gataaactca gtccagcaac acaaaaccgc aacacattgc
    11401 attttgtccc gtagaaaaga cttgtatgtg tgagcttgtt ttctgcgcct acgcagataa
    11461 ggattgagaa tgccgcgcac tgtaacacat aatccggata gccccaataa tgacgatgtt
    11521 ttagccgcat ctgaaaaatg ggacgcctgt aaacccccct ataccagcgc acacatgaaa
    11581 atctgtgttg ctgccgccaa aatcatcctc gctgcttccg gcgtggctcg ccgttccaaa
    11641 tacgaaaaag agaactatct ccgtatcgat ttcagcaaag ccggtaaggt tacattttac
    11701 gccgagtttc caaaaaagat gggcctcaaa ggtaaaaagc tcggcgagtg gccggagctc
    11761 gctatccagc tggcgcgcga aaaagcacta ggtatggctg acggtggact gcgggcagag
    11821 tccgtacatg cagcgctgga aatgtaccgg gatgacctca aagccaaagt agcccggcag
    11881 aagctgagcc cggacagttt cacaacctac ggggtgcgta tcgaccggat taaagcaacg
    11941 ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc ggctggtgga agtgctggac
    12001 gagtggatcg ccactcgctc gaacaataac gccctggagt tgtttgccga gctccgtcgg
    12061 ttctggaagt tctgcgcacc tactctttgc aacggccgca atgttgccgc cagtctgcca
    12121 gatgattatg tttcctcccg cgtacagaaa cctaccccca cacggctttt taccgatatt
    12181 gaatcaatcg cccgactctg gctcaatgtt gctgcctgca cctctgtaca ccagaagaat
    12241 gctgttcgct tcatgatcat cactggtgtt cgtccgatta atgtccataa cctgcgctgg
    12301 gactacgttt acgaggaggc tggtgaaatt gtttatccgg aaggggttat cggcatgcga
    12361 ggggctatga aaacacaaaa ggctttccgc ctgccgataa cgcctgagat ccggcggatt
    12421 atcgacgagc agaaagcctg gcgtgattca gttcctgagt gcaacaggga ttatgtattt
    12481 ttgcagccac gtgatccaat gcagccattt tcaaaacgat cactggataa gctggtgaaa
    12541 acatacagcc cggacggggc tgtaaaagga ataaaacatg atgggactgt taaagggaaa
    12601 gacggtgcat ttaatacgat gtgccgtaaa ttccttaaga gcaatgttat tgccttgatc
    12661 gataggaatt aaaaccccaa aaagattaaa aaaacaccac aaaacggatg tttcttcaac
    12721 accacttttg ctcatatgaa cggaaccgac gattaaactg gatggctctg attgattcag
    12781 ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg gacgcaactt cccctctgcg
    12841 gctctcagcc gcaccaccgc atccgggcca gcagctcatg catcaggacc tgctctgcca
    12901 gacggtagcc ccgcttcagc cccgtaaaac gcatctgact cccgcacagc acgcacttca
    12961 gcgggtcaac cttcagtaac ctctgataca tccctctcca ggtgatttgc atcgccgttt
    13021 ttctcactgt ctccgttatg atgtacacca cttcttccag taaccgccgt ttcgccggac
    13081 tcaaaaaacc gtagtacctc accatacgga accccttatc cgccacatgc caggagaacc
    13141 tttccatgaa ctcatctcca ctcatcaaca ggtattcttc ccgttttgtt cggtgactgt
    13201 tgtaacgcag accgatttca tcctgaccg
//
LOCUS       DAZPAC010000112        13038 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZPAC010000112 DAZPAC010000000
VERSION     DAZPAC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13038
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.112"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHUPM_002662"
     CDS             complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHUPM_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR0870395.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1598..4654
                     /locus_tag="ACHUPM_002663"
     CDS             1598..4654
                     /locus_tag="ACHUPM_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR0870396.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4651..5061
                     /gene="menI"
                     /locus_tag="ACHUPM_002664"
     CDS             4651..5061
                     /gene="menI"
                     /locus_tag="ACHUPM_002664"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR0870397.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5161..5349
                     /locus_tag="ACHUPM_002665"
     CDS             5161..5349
                     /locus_tag="ACHUPM_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR0870398.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5897..6265
                     /gene="sufA"
                     /locus_tag="ACHUPM_002666"
     CDS             5897..6265
                     /gene="sufA"
                     /locus_tag="ACHUPM_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR0870399.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6274..7761
                     /gene="sufB"
                     /locus_tag="ACHUPM_002667"
     CDS             6274..7761
                     /gene="sufB"
                     /locus_tag="ACHUPM_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR0870400.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASELGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7771..8517
                     /gene="sufC"
                     /locus_tag="ACHUPM_002668"
     CDS             7771..8517
                     /gene="sufC"
                     /locus_tag="ACHUPM_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR0870401.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8492..9763
                     /gene="sufD"
                     /locus_tag="ACHUPM_002669"
     CDS             8492..9763
                     /gene="sufD"
                     /locus_tag="ACHUPM_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR0870402.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9760..10980
                     /gene="sufS"
                     /locus_tag="ACHUPM_002670"
     CDS             9760..10980
                     /gene="sufS"
                     /locus_tag="ACHUPM_002670"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR0870403.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10993..11409
                     /gene="sufE"
                     /locus_tag="ACHUPM_002671"
     CDS             10993..11409
                     /gene="sufE"
                     /locus_tag="ACHUPM_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR0870404.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHUPM_002672"
     CDS             11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHUPM_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR0870405.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHUPM_002673"
     CDS             complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHUPM_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR0870406.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         3270 a         3216 c         3504 g         3048 t
ORIGIN      
        1 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcggtg
       61 attaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
      121 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
      181 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
      241 cccgaaagca atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc
      301 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
      361 cactaacaat accgttcccc aggtggtatc gccggtccag tagagccaga taatcgccgg
      421 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
      481 caacgttgca taaggtacgc cggatacggc gaggccgata ctgccaagca ctgcctgtac
      541 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac
      601 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
      661 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
      721 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
      781 gcggactttc gccatgatcg ccgtaccccc catatccagc aagttgtgcc agcctgcata
      841 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
      901 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
      961 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     1021 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     1081 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     1141 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     1201 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     1261 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     1321 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     1381 cgatttactc gtcgtatttc aagttgcatg cgctgcggct acgttcgttc accccagtca
     1441 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     1501 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     1561 cgacctacat cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc
     1621 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     1681 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     1741 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     1801 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac
     1861 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     1921 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     1981 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcaccag aactttcgac
     2041 cagcaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc agggatcgct
     2101 ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga
     2161 tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta aaaccaatac
     2221 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtctgcaac gccagttaat
     2281 catcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     2341 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     2401 ggcctttatt accgaagcgc ggctggatat tacgcccttg cctaaagtgc gccgtctggt
     2461 gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta tggttgaggc
     2521 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     2581 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcgggct
     2641 gaacattgtg gaatttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgcact
     2701 ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt
     2761 gtgccgcgag ctggcggggg ttgaacgtat ctatgcaatg cgcaaaaaag ccgttggtct
     2821 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     2881 gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     2941 aagctacggt atgttcggtc acgtcgacgc aggtgttttg cacgtccgtc cggcactgga
     3001 tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct
     3061 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     3121 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     3181 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     3241 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     3301 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     3361 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     3421 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     3481 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttacggacgt taattgcccg
     3541 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     3601 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc cagtgcccca tcaaaattga
     3661 tgtgccggag tttcgctctc gtttcctgca gctctatcac acccgttatt tacgcccgct
     3721 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa
     3781 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     3841 catggttgat ttgccactgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     3901 ctcggcaaac atgacgctgg aacagcttga agctctcaat gcagagcaga aagcgcgcac
     3961 ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     4021 tgtccgtctg gttgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     4081 caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga aaaagacggc
     4141 ggatttcctc aaccgtatgg ctaagcttgg gatgccaatg gtgggcgtcg atccggcgct
     4201 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa
     4261 cgtcttactt gcgaatgaat ggctggcaag cgcacttgag tcacatccag tggctacagt
     4321 cagcggtgaa tcatggtatt tctttggtca ctgtaccgag gttaccgctt tgccgggcgc
     4381 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     4441 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct
     4501 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     4561 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     4621 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     4681 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     4741 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     4801 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
     4861 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc
     4921 cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt
     4981 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
     5041 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt
     5101 cattatctgc acagtaaacg gtggtatact gtgcacgtct tgtctaaaag cgaggatgct
     5161 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
     5221 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
     5281 ctgcttactc tctcttctac tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
     5341 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
     5401 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
     5461 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     5521 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     5581 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
     5641 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
     5701 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
     5761 aatcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
     5821 gtttgctggg ttgaactgat aatcattatc actagcatgc tgttatacgc tgaaagcgat
     5881 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
     5941 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
     6001 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
     6061 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
     6121 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
     6181 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
     6241 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
     6301 gatgtcaaaa cctggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
     6361 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
     6421 cgtaatgagc cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag
     6481 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagt
     6541 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acttggcgcg
     6601 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
     6661 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt
     6721 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
     6781 ggtgaggcga tccacgatca tccggaactg gtgcgtaaat atctcggcac tgtggtgccg
     6841 ggaaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
     6901 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
     6961 gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
     7021 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcggtggtg
     7081 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
     7141 ggcgataaca acaccggcgg tattctcaat ttcgtcacca agcgtgcttt gtgcgaaggc
     7201 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat
     7261 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
     7321 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
     7381 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
     7441 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
     7501 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
     7561 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
     7621 caacgcggga ttagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
     7681 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
     7741 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
     7801 gtggaagata aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac
     7861 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcga
     7921 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcgctg
     7981 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
     8041 cccggcgtca gtaaccagtt tttcctgcaa acggcactta atgcggtgcg cagctatcgc
     8101 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
     8161 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
     8221 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
     8281 gaatcggact ccgggctgga tattgacgca ttaaaagtgg ttgccgatgg cgtgaactcg
     8341 ctgcgtgacg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
     8401 atcaagcctg attacgtcca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc
     8461 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc
     8521 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
     8581 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
     8641 atatacgccg ctggaagggc tgaccaatag tcagtttgtc agcattgcgg gagatatatc
     8701 cccacagcag cgtgatgcct tagcgttaac gttagacgcc gtgcggctgg tgtttgtcga
     8761 tggacgttac gtgcccgcgc tgagcgatgc aattgaaggc agcggatatg aagtgagcat
     8821 taacgacgac cgtcaggggt tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
     8881 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
     8941 gccattgctg ttaatgcata tcacccaggg cgtggcaggt gaagaggtga acactgccca
     9001 ttaccggcat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgt
     9061 cagccttaat gatgctcgtc attttaccgg ggcacggttc actatcaatg tcgcagcgaa
     9121 cgcccacttg cagcatatca agctggcgtt tgaaaacccg ctcagtcacc actttgctca
     9181 taacgatttg ttgctggctg aggatgccac cgcatttagc catagtttcc tgctgggtgg
     9241 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
     9301 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
     9361 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
     9421 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
     9481 gaccaacaat aatctgctga tgggcaaaat ggcggaagtg gatacaaaac cgcagctgga
     9541 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
     9601 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
     9661 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
     9721 gctggcccga atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa
     9781 gtgcgggccg actttccggt gctttctcgt gaggtgaacg gtttgccgct ggcttatctc
     9841 gatagcgccg ccagtgcgca gaaaccaagc caggtgattg acgccgaggc tgagttttat
     9901 cgtcacggct acgcggcggt gcatcgcggt attcatacct taagcgccca ggcgaccgag
     9961 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    10021 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ttggggcaac
    10081 agcaacgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    10141 gttccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat ccctctcaac
    10201 cccgacggta cgctgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg
    10261 ctggcaatta ctcatgtctc caacgtgctg ggaacagaaa atccgctggc ggaaataatc
    10321 acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    10381 catccggtgg atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg
    10441 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    10501 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg
    10561 accaaagcac catggcggtt tgaagccggt acacccaata ccgggggcat tattggtctt
    10621 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata acatagccga gtatgaacag
    10681 aatctgatgc attatgcgct atcacagctg gaatctgtac cggatctcac tctctatggc
    10741 ccacaaaaca ggcttggcgt tattgctttt aatctcggta aacaccacgc ctatgatgtt
    10801 ggcagttttc tcgataatta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    10861 ttaatggcct attacaacgt ccctgcgatg tgtcgggcgt cgctggccat gtataacacc
    10921 catgaagaag tggatcgtct ggtgaccggc ctgcaacgta ttcaccgttt gctgggataa
    10981 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    11041 tgcgccaact gggaagagaa atatctctac attattgagc tgggccagcg tctgactgaa
    11101 ttacgcgacg aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    11161 attgtcatgc gccagaatgc gcacggaatt attgaattgc agggcgacag cgatgcggcg
    11221 attgtgaaag ggcttattgc ggtagtcttt attctctacg atcagatgac gccgcaggat
    11281 attgtcagtt tcgatgtgcg tccgtggttt gaaaaaatgg cactcaccca acatctcacc
    11341 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    11401 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    11461 tttacactga cctgcacagg agtgttttgg tttcaggtga acataagcgc catccgacgt
    11521 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    11581 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    11641 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga
    11701 taaaaacctt caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    11761 caataacact atcgtcccgg tgccaaaacc tggtacgacg ataactattc cttcacaact
    11821 gttattacct gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    11881 ttattatccg ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg
    11941 gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac
    12001 gcctacggca ggcattcgtc agcgttcgct ggagcgtggc attaaattac cgccagtcgt
    12061 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    12121 cgaatacctc attcatggca ccagtgcgcc ggacagcgtt ggtttgcgcg tcagttcagg
    12181 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    12241 gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta
    12301 tgttgaagta catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata
    12361 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    12421 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    12481 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    12541 tatgttacgc gcgacgcagt agtggtaaat agcagacaaa aaaatggcgc acaatgtgcg
    12601 ccatttttca cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg
    12661 ctggttagca cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac
    12721 gtcgttgctc agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc
    12781 gattttagcg ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag
    12841 taccagttta gtagctttca ttattaatac cctctagatt gagttaatct ccatgtagcg
    12901 ttacaagtat tacacaaagt tttttatgtt gagaatattt ttttgatggg aaggcactta
    12961 tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt ttagataaaa aaactgagag
    13021 aaaacaggat gcttccat
//
LOCUS       DAZPAC010000113        12998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZPAC010000113 DAZPAC010000000
VERSION     DAZPAC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.113"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..191
                     /locus_tag="ACHUPM_002674"
     CDS             <1..191
                     /locus_tag="ACHUPM_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870407.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            191..1393
                     /locus_tag="ACHUPM_002675"
     CDS             191..1393
                     /locus_tag="ACHUPM_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFR0870408.1"
                     /translation="MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQR
                     ISPAELLVPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDA
                     DYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQ
                     GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISP
                     FYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQ
                     DMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYA
                     VMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRL
                     GEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHUPM_002676"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHUPM_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR0870409.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="ACHUPM_002677"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="ACHUPM_002677"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0870410.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="ACHUPM_002678"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="ACHUPM_002678"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR0870411.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="ACHUPM_002679"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="ACHUPM_002679"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR0870412.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="ACHUPM_002680"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="ACHUPM_002680"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR0870413.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="ACHUPM_002681"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="ACHUPM_002681"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR0870414.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="ACHUPM_002682"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="ACHUPM_002682"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR0870415.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3040 a         3228 c         3577 g         3153 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggcgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc atcaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gattgcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctagtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgcgggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAZPAC010000114        12824 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZPAC010000114 DAZPAC010000000
VERSION     DAZPAC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12824
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.114"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            31..1350
                     /gene="idnT"
                     /locus_tag="ACHUPM_002683"
     CDS             31..1350
                     /gene="idnT"
                     /locus_tag="ACHUPM_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFR0870416.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            1417..2415
                     /gene="idnR"
                     /locus_tag="ACHUPM_002684"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             1417..2415
                     /gene="idnR"
                     /locus_tag="ACHUPM_002684"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFR0870417.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            2493..3995
                     /locus_tag="ACHUPM_002685"
     CDS             2493..3995
                     /locus_tag="ACHUPM_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR0870418.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4114..5196)
                     /gene="lptG"
                     /locus_tag="ACHUPM_002686"
     CDS             complement(4114..5196)
                     /gene="lptG"
                     /locus_tag="ACHUPM_002686"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR0870419.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5196..6296)
                     /gene="lptF"
                     /locus_tag="ACHUPM_002687"
     CDS             complement(5196..6296)
                     /gene="lptF"
                     /locus_tag="ACHUPM_002687"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR0870420.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6359..6409)
                     /gene="ytgA"
                     /locus_tag="ACHUPM_002688"
     CDS             complement(6359..6409)
                     /gene="ytgA"
                     /locus_tag="ACHUPM_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR0870421.1"
                     /translation="MSIIILATISTTQENK"
     gene            6563..8074
                     /gene="pepA"
                     /locus_tag="ACHUPM_002689"
     CDS             6563..8074
                     /gene="pepA"
                     /locus_tag="ACHUPM_002689"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR0870422.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8208..8651
                     /gene="holC"
                     /locus_tag="ACHUPM_002690"
     CDS             8208..8651
                     /gene="holC"
                     /locus_tag="ACHUPM_002690"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR0870423.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8651..11506
                     /gene="valS"
                     /locus_tag="ACHUPM_002691"
     CDS             8651..11506
                     /gene="valS"
                     /locus_tag="ACHUPM_002691"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR0870424.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11562..12758)
                     /locus_tag="ACHUPM_002692"
     CDS             complement(11562..12758)
                     /locus_tag="ACHUPM_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR0870425.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDATYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         3294 a         3241 c         3317 g         2972 t
ORIGIN      
        1 cttttctggc actaataaga gataaagact atgccattaa tcattattgc ggcaggcgtc
       61 gcgctgcttc ttatcctgat gatcggcttt aaagttaacg gctttattgc cctcgttctg
      121 gtagctgccg tcgtcggatt tgccgaaggg atggatgcac aggccgtcct gcactctata
      181 caaaatggta tcggcagcac gctcggcggg ctggcaatga tcctcggttt cggggccatg
      241 ttaggcaagc tgatttctga tacgggcgcg gcacaacgta tcgccactac gctgattgct
      301 acttttggta aaaaacgcgt gcaatgggcg ctagtgatca ccggtctggt tgtgggcctc
      361 gccatgtttt ttgaagtggg ttttgtcctg ctgttgccgt tggtatttac catcgtggca
      421 tcatctggat tacccctgtt gtatgttggc gtaccgatgg tagcagcgct ctctgtaacc
      481 cactgttttc tgccgccaca tccagggcct actgccatcg cgactatctt tgaggctaat
      541 ctcggaacga ctttactgta tggatttatc attaccattc cgacagttat tgtcgcagga
      601 ccgctgtttt ctaaactgct aactcgcttt gagaaagcac caccggaagg cttatttaat
      661 cctcatctgt ttagcgaaga ggagatgccc tccttctgga acagtatttt tgctgcagtg
      721 atcccggtca tcctgatggc tatcgccgcc gtttgtgaaa ttacgttacc gaaaactaac
      781 accgtgcgcc tcttctttga atttgtcggt aaccctgccg ttgcgctgtt tattgccatt
      841 gttattgcga ttttcacact gggccgacgt aatggacgca ccatcgagca aatcatggat
      901 atcattgggg attctatagg cgctatcgcg atgattgtgt ttattatcgc tggcggcggc
      961 gcgtttaagc aggtattagt agatagtggt gtcgggcagt atatttcaca cttaatgacc
     1021 ggaactacgc tttcgccgtt attgatgtgc tggactgtcg cggcgctgtt gcgtatcgct
     1081 ctgggctctg ccaccgtcgc ggccattacc accgcgggtg tggtgttgcc gattatcaac
     1141 gttacccatg ccgatcccgc tttaatggta ctggcaactg gtgcgggcag cgtgatcgcg
     1201 tcacacgtaa acgaccctgg cttctggcta tttaaagggt attttaatct gacggttggt
     1261 gaaacgttgc gtacctggac agtgatggaa acccttattt ctattatggg tttgctgggc
     1321 gtgttagcca ttaacgccgt attgcactga aagaataaaa tattgtgatg tagtttcggg
     1381 ggaagattga agattccccc gacgttggga gaagttatgc gcaatcacag aatttcttta
     1441 caggatatcg ctacgctggc tggcgtaaca aaaatgaccg tgagtcgtta tatccgctcg
     1501 ccgaaaaagg tggcaaagga aacaggcgag cgcatcgcca aaattatgga agagatcaat
     1561 tatatcccta atcgcgctcc cggcatgttg ttgaatgcac aaagttatac cctcggtatc
     1621 ttgattccgt catttcaaaa tcaactcttc gctgacattc tcgcgggaat agaatccgtc
     1681 acctctgtgc ataattatca gacccttatt gctaattaca attacgatcg cgattccgaa
     1741 gaagagtcgg tgattaacct gctctcttat aacattgacg ggattattct ttcggaaaaa
     1801 tatcacacca tcaggacggt gaaatttctg cgttcagcca ccattccggt cgtggaattg
     1861 atggatgtac agggagaacg gctggatatg gaggtcggtt ttgataatcg gcaggctgct
     1921 tttgacatgg tgtgtaccat gctggataag cgagtcaggc gcaaaattct gtatctcggt
     1981 tccaaagatg acacccgcga tgaacagcgt tatcaggggt attgcgatgc gatgatgctg
     2041 cataatcttt ccccattacg catgaatcca cgcgccatct catccattca tttaggaatg
     2101 cagttaatgc gtgatgccct tagcgcaaac ccggatttag acggtgtatt ttgcaccaat
     2161 gacgatattg cgatgggtgc gcttctgctg tgccgcgagc gaaacctcgc cgtgccagaa
     2221 caaatttcca ttgcgggttt tcatgggctg gagattggca ggcaaatgat cccaagcctc
     2281 gccagcgtaa ttactccccg ttttgatatt ggtcggatgg ccgcccaaat gttgctgagc
     2341 aagattaaga ataacgatca caaccacaac actgttgacc tcgggtatca gatttatcac
     2401 ggtaacaccc tttaggttat tttctcttca ttacacatat tctctttgcc ggacgttgcc
     2461 tatactccag gcaggaaact ggaggaaatc tcatgagcga acccctgtta attgcccgca
     2521 cgccggacac agaactgttt ttactgccgg gaatggctaa ccgtcacggg ctgattactg
     2581 gcgcaacggg gacgggtaaa actgtcacgt tgcaaaaact ggcagagtca ttgtcggaaa
     2641 tcggtgttcc ggtgtttatg gccgatgtga aaggcgatct gaccggcatc gcgcaggcag
     2701 gaacggcgtc ggaaaaactg ctcgcaagac tgaaaaatat cggcgtcaac gactggcaac
     2761 cgcatgccaa tccggtggtg gtgtgggata tctttggcga gaaaggccat ccggtgcggg
     2821 cgacggtttc ggatctgggg ccgctgttgc tggcacgact gttgaatctc aacgatgtgc
     2881 aatctggcgt gctgaatatt atcttccgca ttgcggacga tcagggattg ttgctgctcg
     2941 actttaaaga tttgcgggcg attacccagt acatcggcga taacgccaaa tccttccaga
     3001 atcagtacgg aaatatcagt agcgcatcgg ttggtgccat ccagcgcgga ttactgtcgc
     3061 tggaacagca aggcgcagaa cacttctttg gtgagccgat gctggatatc aaagactgga
     3121 tgcgcaccga tgccaacggt aaaggcgtta tcaatatcct cagcgccgag aaactttatc
     3181 agatgccgaa actgtacgcc gccagcctgc tgtggatgct ttcagagtta tatgaacaat
     3241 tgcctgaagc gggcgatctg gagaaaccaa aactggtgtt tttcttcgac gaagcacatc
     3301 tgctgtttaa cgatgcgcca caggtactgc tggataagat tgagcaggtg ataaggctta
     3361 ttcgctcaaa aggcgtgggc gtctggttcg tttcgcaaaa cccgtcagat atcccggata
     3421 acgtgctagg gcagctcggt aatcgcgttc aacacgcttt gcgtgctttt acgcctaaag
     3481 atcagaaagc agtgaaggca gcggcgcaaa ccatgcgggc caatccggcg tttgataccg
     3541 aaaaggcaat ccaggaactg gggaccggcg aggcgttaat ctcgtttctc gatgcgaaag
     3601 gaagtccttc agtggtggaa cgggcgatgg tgatcgcacc ttgttcgcga atggggccgg
     3661 tgacggaaga tgagcgtaat ggcctgatta atcactcccc ggtgtatggc aaatatgaag
     3721 atgaggtgga ccgcgagtcc gcctatgaga tgctgcaaaa aggctttcag gccagtaccg
     3781 agcagcaaaa taatcccccc gtgaaaggga aagaggtggc ggtggatgac ggtattcttg
     3841 gtggattgaa ggatattttg tttggcacta ccggaccacg cggcgggaag aaagatggtg
     3901 tggtgcaaac aatggcgaaa agcgccgctc gccaggtgac gaatcagatt gtacgcggga
     3961 tgttggggag tttgctgggg gggagaaaaa ggtaagtcag acgtgatctg tgcccctcac
     4021 cctaaccctc tccccaaagg ggcgagggga ccgttcgaac tcgtatttga ctttgtcaac
     4081 aataataaag ggagctttcg ctcccttgtt agattacgat tttctcatta acagccacag
     4141 gctgattaag aagaagctgg cgcttggcaa cagtgcgccg atgatcggcg ggatgccata
     4201 aaccaacgtc agcgggccga agatctggtc cagtacgtag aagacaaaac cgaagctgat
     4261 accggtgacc acacgcacgc ccatcggtac gctacgcagt gggccaaaga tgaacgacag
     4321 cgccatcaac atcatcaccg ccacggatag cggctggaag attttgctcc acatgttgag
     4381 ctgataacgt ccggcatcct gaccgctcga cttcagatac ttcacatagt tgtgcaaacc
     4441 actgatggag agtgcatccg ggtccagcgc caccacgccc agtttgtctg gcgtgaggtt
     4501 ggttttccag gtgccgctca ccgtctgcga accggtgatc tgtttcggat tggtcagatc
     4561 agattcatca acctgcgaca gacgccagac tttatgttcc gggtcaaact tcgcggtagc
     4621 ggcataacgt acggattgca gacgacgatt ctcgttaaag gcataaatac tgatgccacc
     4681 taactcttcg tcacctttaa cccgctcaat gtagacgaag ttgttgccat ctttcgccca
     4741 taagccttgc tgagtggaga gcaacgagcc gccgtacatc gcctgcgcac ggtagttacg
     4801 cgccatctgc tcgccctgcg acgcgaccca ttcaccaatc gccatcgtca gcaagaccag
     4861 cggaatggcg gttttcatca ccgacagcgc cacctgcata cgggtaaaac cagaagcctg
     4921 catcaccacc agttcgctgc gctgcgccag catcccaaga ccaagcaacg ccccaagcag
     4981 agccgccatc gggaagaaga tttgcacatc tttcggcacg ctcagcaagg tatacattcc
     5041 tgcgcctaac gcgtcgtaac tcccctgccc ggcttttttc agctgatcga caaacttgat
     5101 aatgcccgac agcgacacca gcatgaacag cgtcatcatg atggtggtga aaatagtttt
     5161 accgatatag cggtcaagta cgccaaaagg ttgcatcaca ccgctccttt acgcgaaaaa
     5221 ctggcgcgca ggcggcggac cggcacggtg tcccaaaggt tgagaacaat cgccaaagcc
     5281 agataaatca ggttaacggt ccacatccac agcgtcgggt ccagcttacc tttaccgccg
     5341 ttcgatttca gggaggtctg gatcaggaag aaaagtagat acagcagcat ggctggcagc
     5401 atcgacagta cgcgtccctg acgcgggtta accacgctca acggtacaac cataagtgcc
     5461 atcataaaca cggtgaatac caacgtgata cgccagttca gttctgcgcg ggcacgatcg
     5521 gtgtcagtgt tccacaatgt gcgcatgtcc atctggtcgg tatcgttcgg gtcgagcacc
     5581 accgcctggt gaccaatgat cgcctgataa tcctgaaagt ccgtaatgcg gaaatcgcgt
     5641 aacagtgcag tgccttcgaa gcgcgttccc tggttgagag tgacgacctg ggagccgtcg
     5701 cgcagctggg ttaaatgtcc ggaatcggcc accaccacag aaggacgtgc attacctttt
     5761 ggtcgaattt gtgcgaggaa cacatctttg aaatcgctgc cgtcaacgct ttcgatgaac
     5821 agcaccgagc tgccattagt cgcttgctgg aattgccctt gcgccagcgc cgccatgcca
     5881 gggttcgctt tcgcttctgc taacacttca tcctgatgac gcgatgacca tggtcccgcc
     5941 cacatcacgt taaccgccgc gacgattgcc gtgaacaccg caaggatcat tgccgctttc
     6001 accagcaccg ctttgctcag accgcaggca tgcattaccg taatttcact ttcggtatac
     6061 agtttgccca gcgtcatcag cagcccgagg aacaggctta atggcaggat aagctgcgcc
     6121 atttccggca cgcccaaccc gagaagggag agcaccagat tcgccggaat atcgccgtca
     6181 accgctgcgc cgaggatcct cactaacttt tgacagaaga agatcaaaag caagatgaag
     6241 agtatcgcca gctggctttt gagcgtctcc cgcaccagat atcttatgat tatcacttta
     6301 aatacgcccg taaaaactcg tcttttgcag gattttagct tgtttcatgg cttaaacgtc
     6361 atttattctc ttgagtcgtc gaaatcgtcg ctaagataat tatactcaac ggattcacct
     6421 ctcagatttt gttctgacgt gccaatgccg taataacgtt aagattaaca cgaagtcatc
     6481 gcaacagcgg acatgagtta cgaaagcttg caattctatc tgtagccacc gccgttgtct
     6541 ttaagattca ggagcgtagt gcatggagtt tagtgtaaaa agcggtagcc cggagaaaca
     6601 gcggagtgcc tgcatcgtcg tgggcgtctt cgaaccacgt cgcctttctc cgattgcaga
     6661 acagctcgat aaaatcagcg atgggtacat cagcgccctg ctacgtcggg gcgaactgga
     6721 aggaaaaccg gggcagacac tgttgctgca ccatgttccg aatgtacttt ccgagcgaat
     6781 tctccttatt ggttgcggca aagaacgtga gctggatgaa cgtcagtaca agcaggttat
     6841 tcagaaaacc attaatacgc tgaatgatac tggctcaatg gaagcggtct gctttctgac
     6901 tgaactgcac gttaaaggcc gtaacaacta ctggaaagtg cgtcaggctg tcgagactgc
     6961 aaaagagacg ctctacagtt tcgatcagct gaaaacgaac aagagcgaac cgcgtcgtcc
     7021 gctgcgtaaa atggtgttca acgtgccgac ccgccgtgaa ctgaccagcg gtgagcgcgc
     7081 gatccagcac ggtctggcga ttgccgccgg gattaaagca gcaaaagatc tcggcaatat
     7141 gccgccgaat atctgtaacg ccgcttacct cgcttcacaa gcgcgccagc tggctgacag
     7201 ctacagcaag aatgttatca cccgcgttat cggcgaacag cagatgaaag agctggggat
     7261 gcactcttat ctggcggtcg gtcagggttc gcaaaacgaa tcgctgatgt cggtgattga
     7321 gtacaaaggc aacgcgtcgg aagatgcacg cccaatcgtg ctggtgggta aaggtttaac
     7381 cttcgactcc ggcggtatct ccatcaagcc ttcagaaggc atggatgaga tgaagtacga
     7441 tatgtgcggt gcggcagcgg tttacggcgt gatgcggatg gtcgcagagc tacaactgcc
     7501 tattaacgtt attggcgtgc tggcaggctg tgaaaacatg cctggcgggc gtgcctatcg
     7561 tccgggcgat gtgttaacca ccatgtccgg tcaaaccgtt gaagtgctga acaccgatgc
     7621 cgaaggccgc ctggtactgt gcgacgtgtt aacttacgtt gaacgttttg agccggaagc
     7681 ggtgattgat gtggcgacgc tgaccggtgc ctgcgtgatc gcgctgggtc atcatattac
     7741 tggtctgatg gcgaaccata atccgctggc ccatgaactg attgccgcgt ctgaacaatc
     7801 cggtgaccgc gcatggcgct taccgctggg tgacgagtat caggaacaac tggagtccaa
     7861 ttttgccgat atggcgaaca ttggcggtcg tcctggtggg gcgattaccg caggttgctt
     7921 cctgtcacgc tttacccgta agtacaactg ggcgcacctg gatattgcag gaaccgcctg
     7981 gcgttctggt aaagcaaaag gcgcaaccgg tcgtccggta gcgttgctgg cacagttcct
     8041 gttaaaccgc gctgggttta acggcgaaga gtaagtttgc gttttaccct caccctaacc
     8101 ctctccctaa gggagaggga accgatcgag cgcaatagaa attcattgct catctttcag
     8161 ggtgaggaat gcagcaatat ttaaatccac cacaagaagc cccatatatg aaaaacgcaa
     8221 cgttctacct tctggacaat gacaccaccg tcgatggctt aagcgccgtt gagcaactgg
     8281 tgtgtgaaat tgccgcagaa cgttggcgca gcggtaagcg cgtgctcatc gcctgtgaag
     8341 atgaaaagca ggcttaccgg ctggatgaag ccctgtgggc gcgtccggca gaaagctttg
     8401 tcccgcataa tttagcggga gaaggaccgc gcggcggtgc gccggtggag atcgcctggc
     8461 cgcaaaaacg cagcagcagc ccgcgcgata tattgattag tctgcgaaca agctttgcag
     8521 attttgccac tgctttcaca gaagtggtag acttcgttcc ttatgaagat tctctgaaac
     8581 aactggcgcg cgaacgctat aaagcctacc gcgtggctgg tttcaacctg aatacggcaa
     8641 cctggaaata atggaaaaga catataaccc acaagatatc gaacagccgc tttacgagca
     8701 ctgggaaaag cagggctact ttaagcctaa tggcgatgaa agccaggaaa gtttctgcat
     8761 catgatcccg ccgccgaacg tcaccggcag tttgcatatg ggtcacgcct tccagcaaac
     8821 catcatggat accatgatcc gctatcagcg catgcagggc aaaaacaccc tatggcaggt
     8881 cggtactgac cacgccggga tcgctaccca gatggtcgtt gagcgcaaga ttgccgcaga
     8941 agaaggtaaa acccgtcacg actacggccg cgaagctttc atcgacaaaa tctgggaatg
     9001 gaaagcggag tccggcggca ccattacccg tcagatgcgc cgtctcggca actctgtcga
     9061 ctgggagcgt gaacgcttca cgatggacga aggcctgtcc aatgcggtga aagaagtttt
     9121 cgttcgtctg tataaagaag acctgattta ccgtggcaaa cgcctggtaa actgggatcc
     9181 gaaactgcgc accgctatct ctgacctgga agtggaaaac cgcgaatcga aaggttcgat
     9241 gtggcacatc cgctatccgc tggctgacgg cgcgaaaacc gcagacggta aagattatct
     9301 ggtggttgcg actacccgtc cggaaaccct gttgggcgat accggcgtgg ccgttaaccc
     9361 ggaagatccg cgttacaaag acctgattgg caaatatgtc attctaccgc tggttaaccg
     9421 tcgtattccg atcgttggcg acgaacacgc cgacatggaa aaaggcaccg gctgcgtgaa
     9481 aatcactccg gcgcacgact ttaacgacta tgaagtgggt aaacgtcacg cgctgccgat
     9541 gatcaacatc ctgacctttg acggcgatat ccgtgaaagc gcccaggtgt tcgataccaa
     9601 aggtaacgaa tctgacgttt attccagcga aatcccggca gagttccaga aactcgagcg
     9661 ttttgctgca cgtaaagcag tcgttgctgc ggttgacgca ctcggcctgc tggaagaaat
     9721 taaaccgcac gacctgaccg ttccttacgg cgaccgtggc ggcgtagtta tcgaaccaat
     9781 gctgaccgac cagtggtacg tgcgtgccga tgtactggcg aaaccggcgg ttgaagcggt
     9841 tgagaacggc gacattcagt tcgtaccgaa gcagtacgaa aacatgtact tctcctggat
     9901 gcgcgatatt caggactggt gtatctctcg tcagctgtgg tggggtcacc gtatccctgc
     9961 atggtacgac gaagcaggta acgtttatgt tggccgcaac gaagaagaag tgcgcaagga
    10021 aaataacctc ggtgccgacg ttgccctgcg tcaggacgaa gacgttctcg acacctggtt
    10081 ctcttctgcg ctgtggacct tctctaccct aggctggccg gaaaacactg acgcactgcg
    10141 ccagttccac ccaaccagcg tgatggtatc cggcttcgac atcattttct tctggattgc
    10201 ccgcatgatc atgatgacca tgcacttcat caaagatgaa aatggcaaac cgcaggtgcc
    10261 gttccacacc gtttacatga ccggcctgat tcgtgacgac gaaggccaga agatgtccaa
    10321 atccaagggt aacgttattg acccgctgga tatggttgac ggtatttcgc tgccggaact
    10381 gctggagaaa cgtaccggca atatgatgca gccgcagctg gcggacaaaa tccgtaagcg
    10441 caccgagaag cagttcccga acggtattga gccgcacggc accgatgccc tgcgcttcac
    10501 cctggcggcg ctggcgtcta ccggtcgtga catcaactgg gatatgaagc gcctggaagg
    10561 ttaccgtaac ttctgtaaca agctgtggaa cgccagccgc tttgtgctga tgaacacgga
    10621 aggtcaggat tgcggcttca acggtggtga aatgacgctg tcgctggcgg accgctggat
    10681 tctggcagag ttcaaccaga ccatcaaagc gtaccgcgaa gcgctggaca gcttccgctt
    10741 cgatatcgct gcaggcattc tgtatgagtt cacctggaac cagttctgtg actggtatct
    10801 ggagctgact aagccggtaa tgaacggtgg caccgaagcc gaactgcgcg gtactcgcca
    10861 tacgctggtg accgtactgg aaggtctgct gcgcctcgcg catccgatca ttccgttcat
    10921 caccgaaacc atctggcagc gtgtgaaagt actttgcggt atcactgccg acaccatcat
    10981 gctgcagccg ttcccgcagt acgatgcatc tcaggttgat gaagccgcac tggccgacac
    11041 cgaatggctg aaacaggcga tcgttgcggt acgtaacatc cgtgcagaaa tgaatatcgc
    11101 gccgggcaaa ccgctggagc tgctgctgcg tggttgcagc gcggatgcag aacgtcgcgt
    11161 aaatgaaaac cgtggcttcc tgcaaaccct ggcgcgtctg gaaagcatca ccgtgctgcc
    11221 tgccgatgac aaaggtccgg tttccgttac gaagatcatc gacggcgcag agctgctgat
    11281 cccgatggct ggcctcatca acaaagaaga tgagctggcg cgtctggcga aagaagtggc
    11341 gaagatcgaa ggcgaaatca gccgtatcga gaacaagctg gcgaatgaag gctttgtcgc
    11401 ccgcgcaccg gaagcggtca tcgcgaaaga gcgtgagaag ctggaaggct atgcggaagc
    11461 gaaagcgaaa ctgattgaac agcaggctgt tatcgccgcg ctgtaatcaa aacacagtga
    11521 tgaaaacgaa ggccggagca tgctccggcc ttttttatct cttacagaaa tggtaaagaa
    11581 ggcataattc cccgtgaaat actggctaag aatcctttat ctggttgttc ttctttattg
    11641 attaaaggaa ggtcatccaa atcgcccagc aaatacgtat tttttgcctg ccagtcaatc
    11701 atccatattt tcagcagtgg ataagccaga ccgcccgtaa tcccggatat caccaccaac
    11761 gcacacatgc gataaagcat accgtggtat gttaaagttg agcgaaaacg aatacgccca
    11821 tcattcagtg acaggttgct cataaaatga tttcgcaacg acaccgttaa ataactggtg
    11881 ctaacagcaa tcccaaaata atagattaac tgcgcgatta tcattttacg ttgcatttcc
    11941 ataaattgct gtaaattctc aatatcatca tttgcatacc cagaactatc atactcattt
    12001 aatatttgat cgaagataat ataaccagca acagcgagaa aaggtaataa agccaaaaat
    12061 gcgagaatgg catattttat acaatatgta gcatctaatt tcaccttgaa acgatgtata
    12121 ccgaaactgg tattactcca gagaaaactc attaccagac tatataaagt gccattaaaa
    12181 ataccaatga aaacaataca aactattgtc atcagaacca cagatataat aacgctactt
    12241 gaactattgg catgtagcat ctttgtcgag ataaagaaaa cagtccccat cccaagggcc
    12301 attaaaatcg gcaagaaaaa ggtcacccac cagaaccctt tcatagagca attaaaacta
    12361 aatcttacgc cgttgagact ggtcatcaag gcctgataac gcagtccttt tgctgccata
    12421 aatataagca aaaccaacag tgacaaagtc aaaacacagc caataagcgg catatctgct
    12481 gacactgtca ttaagattgc gaaatagaaa aaaacaaaaa caagacaact aacaaaaaca
    12541 ttcccaccgg taatcccata agaaaatcgt tgtccgttaa cttccatatt agcatagaga
    12601 taacgcttac atttcattaa tgcccatggt aaatagatac ctagcgttat aatcgttaac
    12661 aacacattca ccacacaaat aagaaaatat ctctcacctg taccatgaaa aacaaacgaa
    12721 tgggacggaa catccatatc attaataact tgagccataa aagttccctg aattaaaaaa
    12781 tccattgata taatttatat atacagacag taaaatgttc tgcc
//
LOCUS       DAZPAC010000115        12690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZPAC010000115 DAZPAC010000000
VERSION     DAZPAC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.115"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(63..950)
                     /gene="rarD"
                     /locus_tag="ACHUPM_002693"
     CDS             complement(63..950)
                     /gene="rarD"
                     /locus_tag="ACHUPM_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR0870426.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1002..1469)
                     /gene="yigI"
                     /locus_tag="ACHUPM_002694"
     CDS             complement(1002..1469)
                     /gene="yigI"
                     /locus_tag="ACHUPM_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR0870427.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1634..2503
                     /gene="pldA"
                     /locus_tag="ACHUPM_002695"
     CDS             1634..2503
                     /gene="pldA"
                     /locus_tag="ACHUPM_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR0870428.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2636..4465
                     /gene="recQ"
                     /locus_tag="ACHUPM_002696"
     CDS             2636..4465
                     /gene="recQ"
                     /locus_tag="ACHUPM_002696"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR0870429.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4529..5146
                     /gene="rhtC"
                     /locus_tag="ACHUPM_002697"
     CDS             4529..5146
                     /gene="rhtC"
                     /locus_tag="ACHUPM_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR0870430.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5208..5828)
                     /gene="rhtB"
                     /locus_tag="ACHUPM_002698"
     CDS             complement(5208..5828)
                     /gene="rhtB"
                     /locus_tag="ACHUPM_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR0870431.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5939..6961
                     /gene="pldB"
                     /locus_tag="ACHUPM_002699"
     CDS             5939..6961
                     /gene="pldB"
                     /locus_tag="ACHUPM_002699"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR0870432.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6969..7769
                     /gene="yigL"
                     /locus_tag="ACHUPM_002700"
     CDS             6969..7769
                     /gene="yigL"
                     /locus_tag="ACHUPM_002700"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR0870433.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7845..8744
                     /gene="bioP"
                     /locus_tag="ACHUPM_002701"
     CDS             7845..8744
                     /gene="bioP"
                     /locus_tag="ACHUPM_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR0870434.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8632..9585)
                     /gene="metR"
                     /locus_tag="ACHUPM_002702"
     CDS             complement(8632..9585)
                     /gene="metR"
                     /locus_tag="ACHUPM_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR0870435.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9821..12082
                     /gene="metE"
                     /locus_tag="ACHUPM_002703"
     CDS             9821..12082
                     /gene="metE"
                     /locus_tag="ACHUPM_002703"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR0870436.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12204..12326)
                     /locus_tag="ACHUPM_002704"
     CDS             complement(12204..12326)
                     /locus_tag="ACHUPM_002704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870437.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(12367..12612)
                     /locus_tag="ACHUPM_002705"
     CDS             complement(12367..12612)
                     /locus_tag="ACHUPM_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR0870438.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         2985 a         3212 c         3404 g         3089 t
ORIGIN      
        1 tttcaaaaaa agataaataa gtcagtgggt aggctcttta aacggcctac ccatgttaat
       61 tcttaacttt ttctacgctg cgtataaatc gcatccatca caaaaattgc cagcgccacc
      121 caaataaagg cgaaagtcac catcttatcg gcacccggtt tttcaccata aaacgtcaca
      181 gccagcagga acatcagcgt cgggccaatg tactggaaaa agcctaacgt tgagagacgc
      241 aagcgcgtgg cagcggcggt aaaacacaac agcggtacgg tagtgacaat accggcggcg
      301 atcagcagta aattcagcga catcgggttt tgccccatat ggctggttga gctgtcggca
      361 atagcaaaca ggtaaattgc cgccacgggc agcagccaca tggtttcgat taacatgccg
      421 gtttgcgctt caacggcaat cttcttgcgt accagaccgt agaaggcaaa actaaatgcc
      481 agtcccagcg cgataatagg tagcgaacca aaagtccaca gctggactaa cacgccacat
      541 atcgccagaa tcaccgccag ccattgcatc cggcggaatc gctcgccgag gaaaatcatc
      601 cccagcacaa tgttcaccag cgggttaata aagtaaccaa ggctcgcttc cagcatatgg
      661 tgattgttca ccgcccagat aaacagtagc cagttgccac caatcagcac ggcagagact
      721 gccagcataa aaattttctg tggcgtctga atcagcgttt ttaaatagga ccactggcgg
      781 caaatgctca tcagcaccac cataaagaaa aacgaccaga tcacgcgatg cgtcaggatt
      841 tcatcggcgg gcacgtagta aatcaacttg aagtacgctg gcgctatacc ccaaataaaa
      901 taagcggcaa gagcgagtaa tacgccctgc cgcgtttgtt ttgcatccat cgggaatact
      961 catttttaat tggtaacagc agtttacctg cttttatgtc ttcaacctac catataggtg
     1021 gcggtggcac tggcaatata aagctgttct tcattgtgta attcaacgcg ggcgacggcg
     1081 actttattgc ctgcacgcaa caggctactg gtagcagtaa aacgctcgcc cctgcctggg
     1141 cgcagataat caacgcgaag atcaatggtc cccatccgcg atagccgctg gcgtagttca
     1201 tcttcactga tggtttcgtg gcgggttaag gtacttccca cgcacaccag accggcggcg
     1261 acatccagcg ccgacgcaat gaccccgccg tgcaaaatgc tttgcgccca gttgcccacc
     1321 atcattggct gatttttaaa ggccagctgt gcgaactctt tttcgtaacg ctccagttcc
     1381 atccccaatg cgcggttaaa tggcatgtga taaacaaaca tctcacccac taatttcagg
     1441 gcttgttcag cggtcagtac ggcagacata tcatccttac acttcattgg ttaatgaaat
     1501 gttgatttta tgcttctttg ttgttggttt ctactttagg aagggataac taacggctat
     1561 ggagttaagt atgtaaaata gcccgcagaa aaatattcac cttatcaata attcgttacg
     1621 gagaacacga ccgatgcgga ctctgcaggg ctggttgttg ccggtgttta tgttgcctat
     1681 ggcagtatat gcacaagagg caacggtgaa agaggtgcat gacgcgccag cggtgcgtgg
     1741 cagtattatc gccaatatgc tgcaggagca tgacaatccg ttcacgctct atccttatga
     1801 caccaactac ctcatttaca cccaaaccag cgatctgaat aaagaagcga ttgccagtta
     1861 cgactgggcg gaaaatgcgc gtaaggatga agtaaagttt cagttgagcc tggcatttcc
     1921 gctgtggcgt gggattttag gcccgaactc ggtgttgggt gcgtcttata cgcaaaaatc
     1981 ctggtggcaa ctgtccaata gcgaagagtc ttcaccgttt cgtgaaacca actacgaacc
     2041 gcaattgttc ctcggttttg ccaccgatta ctgttttgca ggttggacgc tgcgcgatgt
     2101 ggagatgggg tataaccacg actctaacgg gcgttccgac ccgacctccc gcagctggaa
     2161 ccgcctttat actcgcctga tggcagaaaa cggtaactgg ctggtagaag tgaagccgtg
     2221 gtatgtggtg ggtaatactg acgataaccc ggatatcacc aaatatatgg gttactacca
     2281 gcttaaaatc ggctatcacc tcggtgatgc ggtgttgagt gcgaaaggac agtacaactg
     2341 gaataccggt tacggcggcg cggagttagg cttaagttac ccgatcacca aacatgtgcg
     2401 cctttatact caggtttaca gcggctatgg cgaatcgctc atcgactata acttcaacca
     2461 gacccgtgtc ggtgtaggag ttatgctaaa cgacttgttt tgatgaacgg ttgagtggtt
     2521 ggcaaatctg gaacccagca tccaggatta ccctctcaga gactaaaagc attgcagttt
     2581 ctcgcgcagg cgctgaaaat agcgcctgtt tttatttcag gcaatcgggg ggaatgtggc
     2641 gcaggcggaa gtgttgaatc tggagtccgg agctaaacag gttttacaag aaacctttgg
     2701 ctaccaacag tttcgccccg gccaggaaga aattatcgac actgtgcttt ccggtcgcga
     2761 ttgcctggtc gtcatgccca ccggtggcgg aaaatccctt tgctatcaaa ttcctgcctt
     2821 attgctaaac ggccttaccg tggttgtttc accgctgatt tcgttgatga aagatcaggt
     2881 ggatcaactg caagccaacg gcgtggcggc ggcgtgcctt aactcgacgc aaacccgcga
     2941 gcagcaactt gaagtgatga caggctgccg caccgggcaa attcgtctgc tttatatcgc
     3001 cccggaacgc ctgatgctgg ataactttct tgagcatctg gcgcactgga atccggtgtt
     3061 attagccgtt gatgaagcgc actgtatctc ccaatggggc cacgatttcc gcccggaata
     3121 tgccgcgctt ggtcagttac gccagcgttt cccgaccctg ccgtttatgg cgctgactgc
     3181 cacagccgac gacaccacgc gccaggatat cgtgcgcttg ctggggctga acgatccgct
     3241 gattcaaatc agcagttttg accgtccgaa tattcgctac atgctgatgg agaaattcaa
     3301 accgctcgat cagttgatgc gctacgtgca ggaacagcgc ggtaagtccg gcattatcta
     3361 ctgcaacagc cgggcgaaag tagaagacac cgctgcgcgc ctgcaaagca agggtattag
     3421 cgcggcggcc tatcatgccg ggctggaaaa taatgtccgc gccgacgtgc aggagaaatt
     3481 ccagcgcgat gacctgcaaa ttgtggtggc gacggtggcg ttcggcatgg gcatcaataa
     3541 accaaacgtt cgcttcgtgg tccactttga tattccgcgc aatatcgaat cctattatca
     3601 ggaaaccggt cgcgccgggc gtgatggcct gcctgcggaa gcgatgctgt tttacgatcc
     3661 ggctgatatg gcgtggctgc gccgttgtct ggaagagaag ccgcaggggc agttgcagga
     3721 tattgagcgc cacaaactca atgcgatggg cgcgtttgcc gaagcgcaaa cttgccgtcg
     3781 tctggtattg ctgaactatt ttggcgaagg gcgtcaggag ccgtgcggga actgcgatat
     3841 ctgcctcgat ccgccgaaac agtacgacgg ttcaaccgat gctcagattg ccctttccac
     3901 cattggtcgt gtgaatcagc ggtttgggat gggttatgtg gtggaagtga ttcgtggtgc
     3961 taataaccag cgtatccgcg actatggtca tgacaaactg aaagtctatg gcatgggccg
     4021 tgataaaagc catgaacatt gggtgagcgt gatccgccag ctgattcacc tcggcctggt
     4081 gacgcaaaat attgcccagc attctgccct acaactgaca gaggccgcgc gcccggtgct
     4141 gcgcggcgaa tcctctttgc aacttgccgt gccgcgtatc gtggcgctca aaccgaaagc
     4201 gatgcagaaa tcgttcggcg gcaactatga tcgtaaactg ttcgccaaat tacgcaaact
     4261 gcgtaaatcg attgccgatg aaagtaatgt cccgccgtac gtggtgttta acgacgcaac
     4321 cttgattgag atggctgaac agatgccgat caccgccagc gaaatgctca gcgttaacgg
     4381 cgttgggatg cgcaagctgg aacgctttgg taaaccgttt atggcgctga ttcgtgcgca
     4441 tgtcgatggc gacgacgaag agtagtcagc agcataaaaa agtgccagga tgaagactcc
     4501 gtaaacgttt cccccgcgag tcaaatgtat gttgatgtta tttctcaccg tcgccatggt
     4561 gcacattgtg gcgcttatga gccccggtcc cgatttcttt tttgtctctc agaccgctgt
     4621 cagtcgttcc cgtaaagaag cgatgatggg cgtgctgggc attacctgcg gtgtaatggt
     4681 ttgggctggg attgcgttgc ttggcctgca tttgattatc gaaaaaatgg cctggctgca
     4741 tacgcttatg gttggcggtg gcctgtatct ctgctggatg ggttaccaga tgctgcgcgg
     4801 cgcgctgaaa aaagaggcgg tttctgcacc tgcgccacag gtcgagctgg cgaaaagtgg
     4861 gcgcagtttc ctgaaaggtt tactgaccaa tctcgctaat ccgaaagcga ttatctactt
     4921 tggctcggtg ttctcattgt ttgtcggtga taacgttggc actaccgcgc gctggggcat
     4981 ttttgcgctg atcattgtcg aaacgctggc gtggtttatc gtcgttgcca gcctgtttgc
     5041 cctgccgcaa atgcgccgtg gttatcaacg tctggcgaag tggattgatg gttttgccgg
     5101 ggcgttattt gccggatttg gcattcattt gattatttcg cggtgatgcc agacgcgtct
     5161 tcagagtaag tcggataagg cgtttacgcc gcatccgaca ttatttttca cgcatgcctc
     5221 gccgatgcta acagcgctcc caccagcata aacaacgagc cgaaaatctt attcagcgcc
     5281 ttcatctgct ttggtccttt aatccataga gcaatccgtt gagcaagggt ggcgtaaccg
     5341 atcatcacaa taatatcgac cacaatagtg gtgacgccga gcacgatata ctgcatcagt
     5401 tgcggctgtt gcggcatgat gaattgcgga aatagcgccg ccagaaacac aatacttttg
     5461 ggattggtga gattcacaaa aactgcgcgc tggaacaaat gtcgacgcga ttgagtagag
     5521 gccagcgatt taaggtcaat tgcaccagcg gcgcgccact gctggattcc cagccaaatc
     5581 aagtaagccg cgcctgccca cttcaacact tcaaacgcaa tcactgagcg ggaaaatagc
     5641 gtccccaacc ccacgccaac cagcacaata tgaatcgcca gtccggtctg aagcccagca
     5701 atagacgcca ccgcgccgcg ataaccgtgg ttgagcgagg tggtcatagt gttgattgca
     5761 ccagagcctg gcgacaggct taaaatgatc gatgtcagca ggtaggcaaa ccaccattct
     5821 aaggtcatga tgaactcccg gtgtgtctat ttttgtgcca caatacgcta ctgtcgcagc
     5881 gttgtgtcag gcacgctaaa aaaaacgatt ttacgtggtt taagaggcag attacccgat
     5941 gtttcagcag caaaaagact gggaaacaag agaaaacgcg tttgctgctt ttaccatggg
     6001 accgctgact gatttctggc gtcagcgtga tgaagcagag tttactggtg tggatgacat
     6061 tccggtgcgc tttgtccgtt ttcgcgcaca gcaccatgac cgggtggtag tcatctgccc
     6121 ggggcgtatt gagagctacg taaaatatgc ggaactggcc tatgacctgt tccatttggg
     6181 gtttgatgtc ttaatcatcg accatcgcgg gcagggacgt tccggtcgcc tgttagccga
     6241 tccgcatctc gggcatgtta atcgctttaa tgattatgtt gatgatctgg cggcattctg
     6301 gcagcaggag gttcagcccg gtccgtggcg taaacgctat atactggcac attcgatggg
     6361 cggtgcgatc tccacattat ttctgcaacg ccatccaggt gtatgtgacg ccattgcgct
     6421 aactgcgcca atgtttggga tcgtgattcg tatgccgtca tttatggcac ggcagatcct
     6481 caactgggcc gaagcgcatc cacgtttccg tgatggctat gcaataggca ccgggcgctg
     6541 gcgcgcgttg ccgtttgcta tcaacgtact gacccacagc agacagcgat atcgacgtaa
     6601 cttacgcttc tatgctgatg acccaacgat tcgcgtcggt gggccgacct accattgggt
     6661 acgcgaaagt attctggctg gcgaacaggt gttagccggt gcgggtgatg acgccacgcc
     6721 aacgcttctc ttgcaggctg aagaggaacg cgtggtggat aaccgcatgc atgaccgttt
     6781 ttgtgaactc cgcaccgccg cgggccatcc tgtcgaagga ggacgaccgt tggtaattaa
     6841 aggtgcttac catgagatcc tttttgaaaa ggacgcaatg cgctcagtcg cgctccacgc
     6901 cattgttgat tttttcaaca ggcataactc acccagcgga aaccgctcta cagaggttta
     6961 aatttcttat gtaccaggtt gttgcgtctg atttagatgg cacgttactt tctcccgacc
     7021 atacgttatc cccttacgcc aaagaaaccc tgaagctgct caccgcgcgc ggcatcaact
     7081 ttgtgtttgc gaccggtcgt caccacgttg atgtggggca aattcgcgat aatctggaga
     7141 tcaagtcgta catgatcacc tccaacggcg cgcgcgttca cgatctggat ggcaatctga
     7201 tttttgctca taacctggat cgcgacattg ccagcgatct gtttggcgta gtcaacgaca
     7261 atctggacat cattactaac gtttatcgcg acgacgaatg gtttatgaat cgccatcgcc
     7321 cggaagagat gcgctttttt aaagaagcgg tgttcaaata tgcgctgtat gagcctggat
     7381 tactggagcc ggaaggcgtc agcaaagtgt tcttcacctg cgattcccat gaacaactgc
     7441 tgccgctgga gcaggcgatt aacgctcgtt ggggcgatcg cgtcaacgtt agtttctcta
     7501 ccttaacctg tctggaagtg atggcgggcg gcgtttcaaa aggccatgcg ctggaagcgg
     7561 tggcgaagaa actgggctac agcctgaagg attgtattgc gtttggtgac gggatgaacg
     7621 atgccgaaat gctgtcgatg gcggggaaag gctgcattat gggcagtgcg caccagcgtc
     7681 tgaaagacct tcatcccgag ctggaagtga ttggtactaa tgccgacgac gcggtgccgc
     7741 attatctgcg taaactctat ttatcgtaat cgttctttat ttggtcagtt gtcaacctga
     7801 tacttcgcta caatggatac tcgttaatca aagagttttc cattgtggcg ctacttatca
     7861 tcaccacgat tctgtgggcc ttctccttta gcttttatgg cgagtacctt gcggggcacg
     7921 tcgatagcta ttttgcggtg ctggtgcgcg ttggcctggc ggcactcgtt tttctgccgt
     7981 ttctgcgtac ccgtggcaat agcctgaaaa cggtcggcct gtatatgctg gtgggcgcga
     8041 tgcagctcgg cgtgatgtat atgctgagtt tccgcgctta tctctacctg acggtctccg
     8101 aattgttgct gttcaccgta ctgacgccgc tctacatcac gctgatttat gacatcatga
     8161 gtaagcgccg tctgcgctgg ggctatgctt ttagcgcctt gctggcggtg attggtgccg
     8221 ggattattcg ctatgatcag gttaccgacc atttctggac tggcttgttg ctggtgcaac
     8281 tctccaatat cacttttgcc atcggcatgg tgggttacaa acgcctgatg gaaactcgcc
     8341 cgatgccgca gcataacgcc tttgcgtggt tctatcttgg cgcgttcctg gtggcggtaa
     8401 ttgcatggtt cttgctggga aatgcgcaga aaatgccgca aaccacgttg caatggggca
     8461 ttctggtgtt tcttggcgtg gtggcttccg ggattggcta ctttatgtgg aactacggcg
     8521 cgacgcaggt ggacgccgga acgctgggca ttatgaataa tatgcacgtt ccggcagggc
     8581 tgctggtaaa cctggctatc tggcaccaac agccgcactg gccaacgttc attacaggcg
     8641 cgctggtgat cctggcctca ctgtgggtgc atcgtaagtg ggtcgctccg cgctcttcac
     8701 aaacggcaga tgatcgcagg cgtgattgcg cgctgagcga ataaatgctt ctgtaatcgg
     8761 ctgacgctgc tcgccatcgc gcacggcggc gtacagtcgg ctccacaagc cttcgcccag
     8821 ggttttggtc accaccagac cttggcgctc aaaactctct actacccaat gcggtagcgc
     8881 ggcaataccc atccgcgcgg caaccatctg aatcaacaat aaggtgttat cgacgctttt
     8941 cagtgacggg ctgacgcctg ccggctgaag aaaatgccgc cagacatcca gtcgactacg
     9001 ctgcaccgga taaatcaaca gcgtctcgct ggcgagatct tccggtgtaa ttcgcgtttt
     9061 cgccgccagt ggatggtcag gtgctaacac cagacgcact tcatagtcga acatcggcga
     9121 ataatgcagg ccactgcgcg gcaggatatc ggaggtcatc accagatcca gctcgccctg
     9181 ttgcaacgca ggctgcgggt caaacgtcac gcccgattta aaatccatct ctacctgcgg
     9241 ccagttctta tggaaatttt ctaacgcggg cgtcagccac tgaatacagc tatggcactc
     9301 aatggcaatg cgcagacgcg tctgctgcgg ttcattgcag gcttgcagtg cctggctgat
     9361 ttgcggcagc acctggtttg ccagttgcaa caggatttct ccctgcggtg taaagcgtag
     9421 cggctggctc ttacgcacaa atagccggaa gccaaggcgt tgttccagat cgctaaactg
     9481 gtgagacagg gcggattgcg tctgatgcaa cgtcgccgca gcggctgcga gagagccgca
     9541 gttccgcaac gcttgtagcg ttttcaggtg ttttacttcg atcatgaaag tccttcactt
     9601 cggcatgaat aatttgcgct tgaggaatat acagtaaccg ccaattatgg atgtgtaaac
     9661 atctggacgg ctaaaatcct tcgtctttta aatttatggt gcgttggctg cgtttctcca
     9721 ccccggtcac ttacttcagt aagctcccgg ggatgaataa acttgccgcc ttccctaaat
     9781 tcaaaatcca taggatttac atataattag aggaagaaaa atgacaatat tgaatcacac
     9841 cctcggtttc cctcgcgttg gcctgcgtcg cgagctgaaa aaagcgcaag aaagttattg
     9901 ggcggggaac tccacgcgtg aagaactgct gacagtaggg cgtgaactgc gtgctcgtca
     9961 ctgggatcaa caaaagcaag cgggtatcga cctgctgccg gtgggcgatt ttgcctggta
    10021 cgatcatgta ctgaccacca gtttgctgct gggtaatgtt ccgccacgtc atcagaacaa
    10081 agacggttcg gtagatatcg acaccctgtt ccgtattggt cgtggacgtg cgccgactgg
    10141 cgaacctgcg gcggcagcgg aaatgaccaa atggtttaac accaactatc actacatggt
    10201 gccggagttc gttaaaggcc aacagttcaa actgacctgg acgcagctgc tggaggaagt
    10261 ggacgaggcg ctggcgctgg gccacaacgt taagcctgtg ctgctggggc cggttactta
    10321 cctgtggctg gggaaagtga aaggtgaaca gtttgaccgc cttagcctgc tgaacgacat
    10381 tctgccggtt tatcagcaag tgctggcaga actggcgaaa cgcggcatcg agtgggtaca
    10441 gattgatgaa cccgcgctgg tactggaact accacaggcg tggctggacg catacaaacc
    10501 cgcttacgac gcgctccagg gacaggtgaa actgctgctg accacctatt ttgaaggcgt
    10561 aacgccaaat ctcgacacga ttactgcgct gcctgttcag ggtctgcatg ttgatctcgt
    10621 acatggtaaa gatgacgttg ctgaactgca caagcgcctg ccttctgact ggttgctgtc
    10681 tgcgggtctg atcaatggtc gtaacgtctg gcgcgccgat cttaccgaga aatatgcgca
    10741 aattaaggac attgtcggca aacgtgattt gtgggtggca tcttcctgct cgttgctgca
    10801 cagccccatc gacctgagcg tggaaacgcg tcttgatgca gaagtgaaaa gctggtttgc
    10861 cttcgcccta caaaaatgcc atgaactagc actgctgcgc gatgcgctga acagtggtga
    10921 cacggcagct ctggcagagt ggagcgcccc aattcaggcg cgtcgtcact ctactcgtgt
    10981 acataatccg gcggtagaaa agcgtctggc ggcgatcacc gcccaggaca gccagcgtgc
    11041 gaatgtctat gaagtgcgtg ctgaagccca gcgtgcacgt tttaaactgc cagcgtggcc
    11101 gaccaccacg attggttcct tcccgcaaac cacggaaatt cgtaccctgc gtctggattt
    11161 caaaaagggc aatctcgacg ccaataacta ccgcacgggc attgcggaac atatcaggca
    11221 ggccattgtt gaacaggaac gtttgggact ggatgtgctg gtacatggcg aggccgagcg
    11281 taatgacatg gtggaatact ttggcgagca cctcgacggg tttgtcttta cgcaaaacgg
    11341 ttgggtacag agctacggtt cccgctgcgt gaagccaccg attgttattg gtgacgttag
    11401 tcgcccggca ccgattaccg tggagtgggc gaagtatgcg caatctctga ctgataaacc
    11461 ggtgaaaggg atgctgactg gcccggtgac tattctctgc tggtcgttcc cgcgtgaaga
    11521 tgtcagccgt gaaaccatcg ccaaacagat tgcgctggcg ctgcgtgatg aagtggccga
    11581 tctggaagcc gctggaattg gcatcatcca gattgacgaa ccggctctgc gcgaaggttt
    11641 accgctgcgt cgtagcgact gggatgcgta tctccagtgg ggcgtagagg ccttccgtat
    11701 caacgccgcc gtggcgaaag atgacacaca aatccacact cacatgtgtt attgcgagtt
    11761 caacgacatc atggattcga ttgcggcact ggacgcagac gtcatcacca tcgaaacctc
    11821 gcgttctgac atggagttgc tggagtcgtt cgaagagttt gattatccga atgaaatcgg
    11881 tccaggcgtc tatgacattc actcgccaaa cgtaccgagc gtggaatgga ttgaagcctt
    11941 gctgaagaaa gcggcaaaac gcattccggc agagcgcctg tgggttaacc cggactgtgg
    12001 cctgaaaacg cgcggctggc cagaaacccg cgcggcactg gcgaacatgg tgcaggcggc
    12061 gcagaatttg cgtcggggat gatgaatatt aacgagtcac tgtaaattgc agtacgatga
    12121 aagaggcaaa actgcctgat actaggccta cataatttat gcaatttatt gaatttttgg
    12181 gttttgttgg ccggacaagg cgttcacgcc gcatccggca tgaacaacgc gcactttgcc
    12241 aataatctga tggcttccat caccggaagc cattttgcta tctgcgcccg ttcagataca
    12301 gaaataaccg aatcattttt acgcaaaata taatcctgag cgatttgttc agtaatcaac
    12361 gctacgttat ttattctcac ttttcacgaa attgtatccc ttttcagtaa cgtggtagtg
    12421 aggcatagac ttatgtccgc tatcctcaat aataaccaaa ccctcgttag ctaatttgtt
    12481 aacaatgtta acaacattac cgaatcctgg aatttgcctt atggatagtg tgcgatcgag
    12541 attcatccag ttccagttta tatctttctc agccataatt ttcagtacgt taatttcagt
    12601 ttcagttatc atagtcaagt gcatcctggt ggtataagtc attaagttct cccttaagtt
    12661 tataatcttc tagagtagca gtatgcgtat
//
LOCUS       DAZPAC010000116        12576 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZPAC010000116 DAZPAC010000000
VERSION     DAZPAC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12576
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.116"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(760..1791)
                     /gene="repA"
                     /locus_tag="ACHUPM_002706"
     CDS             complement(760..1791)
                     /gene="repA"
                     /locus_tag="ACHUPM_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFR0870439.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     LTNRRRGEHSTKPKCENPSWYRPERYKALKGQLGHAYNRLVKKDPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAEEISPKD
                     SEGHVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDREHRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKKR
                     REKAAASKRANRLKKLPVDQQIYEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
     gene            2749..2907
                     /locus_tag="ACHUPM_002707"
                     /pseudo
     CDS             2749..2907
                     /locus_tag="ACHUPM_002707"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            2945..3232
                     /locus_tag="ACHUPM_002708"
     CDS             2945..3232
                     /locus_tag="ACHUPM_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0870440.1"
                     /translation="MSPQMIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            3674..4207
                     /locus_tag="ACHUPM_002709"
     CDS             3674..4207
                     /locus_tag="ACHUPM_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFR0870441.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIHPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            4461..5144
                     /locus_tag="ACHUPM_002710"
     CDS             4461..5144
                     /locus_tag="ACHUPM_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HFR0870442.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLETAELIFSDEGFQVVRGYEHQFGVERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            5317..5868
                     /locus_tag="ACHUPM_002711"
     CDS             5317..5868
                     /locus_tag="ACHUPM_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870443.1"
                     /translation="MKKQPLLWMTIAAALLSGCTGHREHHPQVTFTVDTVRTDTTASN
                     PICLAEKYGSLLNERHPDLFAGAQGSISRSAFFNDSLTLTPETLHGMIQNTPCVESLV
                     TSVGPDTFTADVSPGVRMQVSPDIHGAPGNITLRLSTTDQKRKQTVEQSISLRPEQSL
                     VVNGFTGDGAGEKRVVLITPHVR"
     gene            6030..6284
                     /locus_tag="ACHUPM_002712"
     CDS             6030..6284
                     /locus_tag="ACHUPM_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFR0870444.1"
                     /translation="MPQQHPGRLQILVVDAHCKRRLFSTKTPTDPDELARRFCTPDNC
                     LVVVLRDNRFLFRLERAPGSHCRWHKGSSSRHQHLQDWLS"
     gene            6451..6747
                     /locus_tag="ACHUPM_002713"
     CDS             6451..6747
                     /locus_tag="ACHUPM_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilJ"
                     /protein_id="HFR0870445.1"
                     /translation="MFTMFFLVKLTIAIVIVCGILSPILVSPHNKLSTLFWKIPNAFW
                     IFLICIYFAIAMAVLIESSEQTRIRRQQYFQQYEVPNETHKNVVIENSIKSQLI"
     gene            6744..7337
                     /locus_tag="ACHUPM_002714"
     CDS             6744..7337
                     /locus_tag="ACHUPM_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HFR0870446.1"
                     /translation="MKNNNVPLYLWLLWCTLCLYITTPWQYLRYPYTDNLSLPYMLYF
                     CVVVVICIFPLLFRCVLLIVRSASKYDFTFFVKKKKGSKKSTIHLSPYADTRCPKSMR
                     KHWDVLLSLISKAINNDEIIVMHSHLFTPARINKLIKRLESYDLSFSVITYPRKTSLY
                     EKLSIPFIYLLFQWSFPHLHSDGMTVVIRPLQPPHTN"
     gene            7371..8438
                     /gene="pilL"
                     /locus_tag="ACHUPM_002715"
     CDS             7371..8438
                     /gene="pilL"
                     /locus_tag="ACHUPM_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HFR0870447.1"
                     /translation="MKKNCLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAQPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSAAAPAQDTATTISPAAMVTTSTSPESPTTANSQN
                     KPRNPFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            8438..8875
                     /gene="pilM"
                     /locus_tag="ACHUPM_002716"
     CDS             8438..8875
                     /gene="pilM"
                     /locus_tag="ACHUPM_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFR0870448.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            8889..10571
                     /locus_tag="ACHUPM_002717"
     CDS             8889..10571
                     /locus_tag="ACHUPM_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFR0870449.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            10564..11859
                     /gene="pilO2"
                     /locus_tag="ACHUPM_002718"
     CDS             10564..11859
                     /gene="pilO2"
                     /locus_tag="ACHUPM_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFR0870450.1"
                     /translation="MADEDINPVVLLEDPKVNHRVWAACLKWTPVVKKQRVPSHHKHK
                     SHVKPRRLTSLKVTVGSRNSRGKISRLTGTGILTRPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLAMNEEPPEKWQVVSSLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAAGAGTVFWMTQPEPDVVPTP
                     EEIAARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFGVIPDFNLKDGARQVSVTRPLPSLPGRDEA
                     VPAPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDEFFPLLQDTGVRLSNIHFELNGGTFSYSSEGHIYASK"
     gene            11846..12298
                     /gene="pilP"
                     /locus_tag="ACHUPM_002719"
     CDS             11846..12298
                     /gene="pilP"
                     /locus_tag="ACHUPM_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFR0870451.1"
                     /translation="MRPSKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVTSSSETVSGFSGVTAPLPSVSEQTAPKNRKELPIIMEINGKD
                     KRLHAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            12309..>12576
                     /locus_tag="ACHUPM_002720"
     CDS             12309..>12576
                     /locus_tag="ACHUPM_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0870452.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PGINPKVVSLSELQEAGQDEKNRGPKERIIHLKDLADVSESEKKV"
BASE COUNT         3339 a         3190 c         2855 g         3192 t
ORIGIN      
        1 tgattgatag atttcacctt acccatcccc agccctgcta agccataccc gctttcagcc
       61 atgagagagt ttctgtgcgc ggtcggagtg gtcccgacga gggtttaccc gaagtcgggg
      121 cgtatctccg cgttagcggg ccgtcagggc cgcttacgag cgtgtacgga ggacttccag
      181 ccagaagact ggcagcgatg atgctgtgta gttacaacat tcataattaa aagcgatgtt
      241 gttcccgccc tttgggcggg ggcggcctcg cttttcagtt atgaggggtg agcaggatgc
      301 tgcgcttgtg tggtgttttt attcctggtg tctttttgct gtggtttcag aatggtgagg
      361 ttcggggccc gcgcgttagc gtgggaggta cttttaaatt ctgagcggcg cggccttggc
      421 cgctgcaagc gaaggccttt ttgtatttgt tcacagtggt ctcagagata aagactttcc
      481 ctgcggcgaa gcaacgcagg ggattaaatg atggggttaa gaatatattg caggtttgat
      541 gtttcaggtt tgcagcttct taaagttctt tgtttgcgga tttatgtgga taactgaaac
      601 aacgtggcca cgttctgatg actgatgcgt ggttttatgg gcagatatgc ccctctgagg
      661 ggatttaagg tgactactaa ggattttggg ctgagcggaa tctgcgccga aagccccgga
      721 ggggctggcg atgattcagt gctgagggaa tgatgctgtc tagtgcggtg gcggggcagc
      781 cagcgtcagt tcaagctgac gcaactccct gatggccagt cgcttcagat ggtcatcgga
      841 acagaaatag gcttcatccg gcggcagacg cttccggaga tactcagcca tctcataaat
      901 ctgctggtca acaggtaatt tcttcaggcg gttggcacgc ttgctggctg ccgctttttc
      961 acggcgcttc ttcagggcat cctggctgcg ctgcagatac cagcgttttc tggccgcatg
     1021 aacggagata tcttcatcct cacgcagaac accttcccga atgagctgct ggcggatttc
     1081 actttcacgc agtcgtttct gctgctgttc gtgaagtttt accatgtcga cgcccagcat
     1141 ctgccagcct gccggtgtta tccagacgta acgtggcaga cgctgacgat gttcacggtc
     1201 ccacattgtt tcctctgaca cacccagcac accaaagcgc acctgttcgg ccagcagacg
     1261 ggaaagacgg gagaccgtca cttccagttc cggaataaca tgtccctcgc tgtctttcgg
     1321 gctgatttct tcagccagcc gagtgacgct catccccacg gtatgtgtgc cggcatcact
     1381 gaagctgacc agaaccggcc agatggcatc gaggagacgt tgtttttccg gccggaaagc
     1441 gtatttgcga ccgacgaacg tccgtttctg tacataaaaa ggatgccgcg acatgcgcat
     1501 gcgcaggctc tgctcgccgg ttacagggtc ctttttcacc agacggttat aggcgtgccc
     1561 gagctgccct ttcagcgctt tatagcgctc aggacgatac caggacggat tttcgcattt
     1621 gggtttagtg gagtgctcac cacgacggcg atttgtgagc ttcctttccg gttcaaccag
     1681 gaaagtggtc gcccttcccg cttcatagtc ttcccagaaa cggatctgct cttcgggtgc
     1741 taactgactc cagtgtactg cgttatatgg agtattcttc agacctgcca caagctgtcg
     1801 cttatggcgg agcgattgtg ccgtgtattt atacactgca cagggttgaa tctctggtac
     1861 ggtttcatta tactttaccc gtattctgtg aggcccccac tatctttcgt cgttccgcca
     1921 agttcgtaag aaagatagtg ggggttttcc ttttatggct tccggaacat cttcagtctt
     1981 ctgacttact gcccgtacat gccatctctc ctgaatgcct tatggcgcat tctgccgtcc
     2041 ggctttcgcc gctttgctcc acagagtgcc accctctgca cagctaataa cagccgaaat
     2101 atacccgctt tacgttaatt tgaaaagcat tacaacgatc tcttctcttc cagtgcctcc
     2161 tcactgatac ctctaataag ttaatcttga cctgcgtcaa aaaaataaaa tctattttta
     2221 aaagcagatt tttctgcttt agatctttct tttggattaa aagatcgact tttgtcctac
     2281 tcacgatcat ttatcttaca accgatctac ctaacagaat aatattctgt tttaacgcaa
     2341 ttaaacgatc taaaaaacct ttaaaaaatc attttgtaga tcattcgatc gtaaatagaa
     2401 taattcttag ataataaaac catttgttac acagcccgcc aattgtgtaa caacagcctc
     2461 tacaagagca tatttgttca tatgttcgtc agaactgcgg attgtctgga cgagggtcag
     2521 acaccgtttc gtgcatatgg accagcatgt tcgaacgcca caaagcagaa gcatcatgcc
     2581 tggatcgctt cggcgcggag cgttaagttt tgacgctttt ttgtatgtct tttaagcggt
     2641 tagttgactc ggtacgttat actaagtacc ctttactggt cacaggcaaa actgtgatcg
     2701 ggcgtaggaa cccgcgtagt catcgtcggc gcagaagtta atttcttcat ttggcgtcgt
     2761 tttttgccat accataaaga caggaacttg tatttcttgt cgtcatctat ggtagctcag
     2821 gcggggcagg cattagcctg gccggtttcc gatgacgccg gtattcctac ccccgtctgg
     2881 gctatcacca atgagcgtag gaactcggtg atagcgtttt tagttgtcat cggagaccgt
     2941 tgctatgtca cctcaaatga tacagaaact cccggccatc actctgttgg aaggcatgtt
     3001 tcccgagcta tcaacaaacc agcttaaagt ctgcgttttt tacgcaatgg gtgttcctta
     3061 cgacgccatt gctcagaact gtcggctttc tcccgaaacg gtacgaacct acctgaagcg
     3121 aagcctgaag aacttaaatc tggaaggata tgatgcactt cgctctgctg tgctgatgcg
     3181 aacgttcgtt ttcatgatta gcaatacagc taaagaaaac gaaaaaatgt gaacaggtgt
     3241 ccccattaaa ggggacactt tcatggaaat tgcattttgc actttacagg cgatcccaaa
     3301 ttgataaaca taaacaccaa gcacaagtta ctttaataaa gcaaatcact tcagacggtg
     3361 tcccagttat ttgtatctgg ctcatgcccg atatatggcg accttactct gaactgacgt
     3421 gctgatgttt tatttcgcgt atcacaatac tgtgccactg tattgctgat attagtgctt
     3481 agccgttctt tatctgtgag ttcgccaaat caccgataca gaacgtacta gagtctgctg
     3541 gttatatgcc tgtcagaact gcagtttatc cagatatggg ctggacactg cttcagacat
     3601 atgatttagc atattcgcct gacatcgcaa aatcgtccat catggatcgg ttttgcgcgg
     3661 agcgttgaac ttcgtgaata tagaacacct gaataaccgg aactggtatc ttgcgcaata
     3721 taatacagcc ggcaaaaaca gagaatcttt attctcatgg ctgaatgagc aaaacgtcgt
     3781 accatggact ccattaataa caagaaagat aagacgggca gactccaggt gctgttaccg
     3841 tgaaagaata ttcgctattt ttccagggta cttttttatt ctggctaatt ttgacattca
     3901 cccagtatca gcattacgtc gacatagcgc cttcattgat ttcgtgaagt ttggcggtga
     3961 aataaaaccc gtcaataaag atattgttga tggattaatg aagatttacc cggaccctgt
     4021 gctaaatccg ggagccaggg aagagctgaa tgcggcttcc agcatatggc tgacaaaagc
     4081 acaatatcag tatctcctca gaatggaaaa tactctccag cctgagtcga ggatatccct
     4141 gctcctggaa ctggtatcaa atgccgaaca tcacggcttt atggagcgcc ttgtaaacat
     4201 cccatagcca gtatttcccg gccttcgggc cgggcttttt tcaattccag agaatattcc
     4261 ggagtgaagc ctgcacgaag ccatccccac catggactcc ccctgtcttt tgatggaacc
     4321 gtgtatttct taatggcgaa agaaataaaa ggcttcaccc cagagtattg attcgtttta
     4381 tcaaaggccg gcccgtgcca tcacgtgtgg cggagagaac ggataccggt cttcttttca
     4441 tctcacagta ggacattcgc atgaccacga cagaacatcc cgtgcgccag ttgctctggt
     4501 gtgcactgaa tgccctgaaa acggcacagg aaaaccacaa tataaccagt gaaaccggat
     4561 tacggcatta cctgcttgaa tggctttctg gtgccagcag acatcctgag ttccgtagtc
     4621 tccctggcga aatcatggcg ttaaaagcat tgaccgaaaa agatcgcaat attcccatca
     4681 tcggaacgct gaatacgctc tttctcagct ctgccactgt cggggaatgt ccgttgtttc
     4741 gttttcgggc tgcactgggt aaattacaga aagcaggctg gcgcacatat gtctgcccat
     4801 ggcctgagag ggtgttcaat gaatccattg aacgggcctg ttccgggaaa cgtcatctgc
     4861 tacaactaag tcgcaccgaa gagtgttttc taccgacagg cgatatgagg gcaccagtta
     4921 cgttgcagct gatcactcca ctacaccgca aaactatgca gcttctggaa acggcagagt
     4981 taatattctc cgacgaagga tttcaggtgg tgagaggata cgaacatcaa ttcggggtcg
     5041 aacgcagaga aaccctcttt cacaccctat ttatcgggct gcccagcctg ccagaagatg
     5101 tctggggtgc aacacaggag cagacgacta tcacattaca ctgaggatgt gtaatccccg
     5161 gcataccccg tttccagtct tcgcagcctg taccgccctt cctgccgttt ccgacattct
     5221 ttctgtcact actcgcttgt gccatttttt cgcaagcggc ccccatcgac atgacggtgc
     5281 gagtctttct ttttaccctg agaggaaatt aacagcatga aaaaacaacc actactctgg
     5341 atgacgatag ccgccgcact gctgtccggc tgcacaggcc acagggaaca tcacccgcag
     5401 gtgacattca cggtcgatac cgtgcgaaca gacacaacgg ccagcaaccc aatctgcctg
     5461 gcggaaaaat acggaagcct tcttaatgag cgccatccgg acctgtttgc cggtgctcag
     5521 gggagtattt cacgcagtgc attttttaat gacagcctga cgctgacacc tgaaacgctc
     5581 cacgggatga tacagaatac gccctgtgtt gaatcactgg taacgtccgt aggtccggat
     5641 accttcactg cagatgtctc tcccggtgtc agaatgcagg tatcaccgga tattcacggt
     5701 gcgcccggaa atatcactct gcgactctcg accacagacc agaaacgaaa acagactgtt
     5761 gaacaaagca tttctctcag accggaacag tcccttgtcg tgaatggatt caccggagac
     5821 ggagctggcg aaaagcgcgt cgttctcatc acacctcacg tgcgttaatt cccaacacaa
     5881 cccgtttccc gtcttcgcag cctgtaccgc ccttcctgcc gtttccgaca ttctttctgt
     5941 cactactcgc ttgtgccgtt ttttcgcaac cggtacgtgc agttatgacg gtacagctgc
     6001 ttcttttccc gttccggaag gagtctttga tgccacagca acatcccgga cgactgcaaa
     6061 tactggtcgt cgacgcacac tgcaaacgca ggctgttttc aacgaaaaca ccgacagacc
     6121 ccgatgagct tgcccgacgt ttctgcacgc cggataactg tctcgtggtg gtactacgcg
     6181 acaaccgatt tctgttccga ctggagcgtg ctcccggcag ccactgccgc tggcacaagg
     6241 gcagcagctc ccgccatcag catctacagg actggctgtc gtaaacacaa caaaaacaaa
     6301 agacctcgtc aaaacaggtg ggggccgtgt tctttttagt taaattaacc atcgcgattg
     6361 cgattgtctg tgtacacctt ttaacgattt taatttagcc gtttgttgcc atctttatta
     6421 cctctctccc ttttatcatg gagattaacg atgtttacca tgttctttct agttaaatta
     6481 accatcgcga ttgtgattgt ctgtggaatc ctttctccga ttttagtttc accacataac
     6541 aaactgtcca cacttttttg gaagatacca aacgcgttct ggattttcct gatttgcata
     6601 tactttgcaa tcgccatggc ggtgttaata gaaagcagtg aacagacacg catccgccgc
     6661 caacaatatt tccaacagta cgaagtgcca aatgagacac ataaaaatgt ggtgatagaa
     6721 aacagtatta aaagtcaact aatatgaaaa ataataacgt accattatat ctatggttgc
     6781 tttggtgtac gctttgtctg tacatcacta ccccatggca atacttacgt tatccataca
     6841 ctgacaactt atcattgcct tatatgttat atttctgtgt ggtggttgtg atttgcattt
     6901 tcccactact tttcagatgt gtacttctta ttgtaaggtc tgcatctaaa tacgacttca
     6961 cattctttgt gaagaagaaa aagggaagca agaaatcaac tatacatctt agcccctatg
     7021 ctgacacacg ttgcccgaag tcaatgagga aacactggga tgtattgctt tctctgatat
     7081 caaaagcaat taataatgat gaaatcattg tcatgcattc acatctcttt actccagcaa
     7141 gaataaataa acttataaag agactggaat cttatgattt gagtttttca gttattacat
     7201 acccacggaa aacaagtctg tacgaaaaat tatcaattcc ttttatatat ctcttatttc
     7261 agtggtcttt cccgcatttg cattcggacg gaatgacagt cgtcattcgt cctctccagc
     7321 cgcctcacac caattaacaa acacgtatat acgaacacgg agtctccgtt atgaaaaaaa
     7381 actgtctgac acttatactg ccgtcattat tgtccggctg tgccggaatt tccggcagca
     7441 ataacgtggc accatcagat acgcctgctc tggtttttgt tgacggacaa atcagtgaaa
     7501 gtgctggcgt tctggcactt acgcaacaac gtctttctcc acctaaaata actccccccc
     7561 cgagagtcag cgtgccagag acagtaaaaa cagcacagcc tgttagcgaa tcccgaccgt
     7621 taccttcacc aacaataagt cagaatttct cccggttaat cattacaggc aagcctggta
     7681 gcgcccctgt aacactatcg ccgacggccc gtaaccggac cgtcagtcag tggataaaaa
     7741 cattactacc ggaaggatgg aaattcagac aagagaatgc agccacgccc aagctgaata
     7801 ccaaactggt ttcatggtct gccaatgatc agtggacccg ttctctgaac cggttgctgg
     7861 aagaacaaca cctatggggc catctggact gggataataa aacgttgacc gtcacaacat
     7921 cagctgctgc accggcacag gacacggcaa ccactatctc gccagcagcc atggtaacca
     7981 catccacttc ccctgaatct ccaacaacag caaatagcca gaacaaacca cgaaatccat
     8041 tcagaggaaa cagcgtatct tcgtcaaccc ccgccgccac cccaactcca accggctcca
     8101 cagtcaagtc tatcccgctc atgaccggga cgcctgtcaa accggtatct cagggtaaag
     8161 agtggcgtgc gccggcaggc accacattaa gagagaacat tattaagtgg gcagaagaaa
     8221 caaaatgcga aagcatggca tcaacgcact ggatggtcat atggccactt tctgtaaccg
     8281 attaccgtct ggatgcgcct ttaatgttcc ggggatcatt tgaatcagcc atggagcagg
     8341 tatttgagtt atatcggact gcgcaaaaac ctttatacgc acaggcttct cgcatgcagt
     8401 gcatcattac agtttctgat acaagggatg gtcgctaatg ggatggctgg tcatggccgt
     8461 cggcctgacc attctgatta tcactggtag ctaccagaat cagaagatgt ctgaaaccac
     8521 aaacgcccag caatatgcaa gtgcgtctgt atgggcaagt cagatactaa tgattgctaa
     8581 cagaatcaat gatatcagat acgtatcagg ccagcaggac ggagtaattt cctcagataa
     8641 actggctctc cctgtcactc cggactcccg aattaaacat cagttacaac aggggcgtct
     8701 gtgggtatgg atgcctgaac aaccaggact tgttgaaact ttgcgttcaa aaagtcgtgg
     8761 ttctgcacta atagggatct tccaaaacgg gcaactcacc tggctatcag gaactgcaac
     8821 cggtctgaca cccccggcag gcattactgc tggttctgtc gtctacgtta actaatctgg
     8881 atatattcat gaaaaaatca caccagcgtt caatgaagct ggcggtgctc ccctgcatga
     8941 tcgccgtcgc tctttccatc tccggatgta ctttcagcga aatcaacaaa atgcagaaaa
     9001 aagcacagga agactcagca catgcacggg aaaaggtatc agccctttcg gcccgtaaat
     9061 cgcaggctct cacctggctc gataatcaat ggataaaccc tgttccggtc gctcaggtat
     9121 caagagagaa aaaacaaaca gctccggcct gctacatcac gcaggcaaga aaaggagaga
     9181 tcactctgca ggaactgggg caacgtatta ctgccgtatg cggcatccct gtaatcatca
     9241 cgcctgacgc agccaattca actcttgaag gaggcgctac ccgccagatg acaggaacac
     9301 taccagcacc agatgaaaat ggacgtctac cgttaagcag cctgggcagc acaacaatga
     9361 ctacctccac tcagccatta acgctgaata acctcatgtg gcagggagat atcaatggtc
     9421 ttctggatct gatggccagc cggagtggtc tgtactggcg catggataat ggtcggattg
     9481 tattctatct gactgaaacc agaacgtatc cacttcatat gctgaacacc aaaaccagca
     9541 gcagttccag tgtcagctct ggctcaacaa gcacaatggg ggcaacagga ggccaggata
     9601 actcagcatc cggtgatgca acgtcctctc agagcacaac cgttggtcag gaatacgatc
     9661 tgtatgaaga catccggaaa actattgaag caatgctgac accagaaaaa ggccgttact
     9721 ggttatctgc atcgagctca acgctaacag tcactgatac tcctgctgtc caggaagccg
     9781 tcgcacgata tgtggacgaa caaaacagta ttatgaaccg ccaggtagcc ctgaacgtac
     9841 aggttctgag cgtcagcaat accagaaacg agcagttcgg tctggactgg aaccttgttt
     9901 acaaatcgct acattccgcc ggagcaacgt tgaacaatgc aagcggagat tttacaggcg
     9961 ctacatctgc aggcgtatca attctggata cggcaacagg gaatgccgcc aaattcagcg
    10021 gttccagcct tctgattaaa gcactgagtg aacaaggcga tgtcagcgtt gtaacttcac
    10081 aagaaagcac tgtcacaaac ctgacgccgg tacctatcca gatggcagat cagacagttt
    10141 acgtcgccca gtcagcaaca actacgacta cggatgtagg agcaacaaca acattaacac
    10201 cgggcatgat caccactgga ttcaatatga ccctactgcc tttaattcag aaaacaggca
    10261 atctccagtt gcagatgaac tttaatctgt ctgatccccc aactatccgt agctttacgt
    10321 caaaagacgg aaacagttac atcgaaatgc catataccaa actgcgttca ctgagccaga
    10381 aggtcaatct gaaagaaggg caatcacttg tcgttactgg tttcgatcag aacaatacga
    10441 cgacaagtaa agccggtacg tttacgccag caaatccatt atttggtggt tcacaaaccg
    10501 gaaaaaatga acgcagcacg cttgtgatca tcattacccc gactttcccg tcaggaggca
    10561 ataatggctg atgaagatat caacccggta gtgcttctcg aagatcctaa agtaaatcat
    10621 cgcgtgtggg ctgcctgtct taaatggact cctgtcgtaa aaaaacagcg cgtcccctca
    10681 catcataaac acaagtctca tgtaaaaccc agacggctta cctccctgaa agtgactgta
    10741 ggcagcagaa attcccgtgg aaagatcagc cgccttaccg gcacaggaat actgaccagg
    10801 ccagaacgca atcattattt ttccctggca ctggctttct gctcatgggt cagaaatgga
    10861 tacggtgtct tccggtacag cgataaggag ctcttattcc ttgcttccat taatggccag
    10921 cctgccgtta tggctgactt atcaggaaat gatgccgacg ttgcccaaaa ggtcagcctt
    10981 tttctggcga tgaacgaaga gccccccgaa aaatggcagg ttgtatcgtc tctggaacat
    11041 cctgataact gggaaagtat tattacccgg ttatcatcag cagacctccg ccgttgcaaa
    11101 cttactgtcg gcaatcgcag caaattcaca ctgcctgccg tactgttttt agtagcagcc
    11161 ggcgcaggca ctgtattctg gatgactcag ccagagccgg atgtcgtacc gacgcccgag
    11221 gagatagctg ctcgggcaag attgcagttt aaaaagcccg atccaccgcc agaactcccg
    11281 catccatggg catcacaacc tgtaatcagt gattttctga aagcctgcgc ggaccttcga
    11341 aaaccgtcac cggtcgcact ggaaggctgg aaacttaccg gcggaacatg tactcctgag
    11401 acattcacac tgatttacga acgacagcct ggaggaacca ttgagggttt tctggcaaga
    11461 agtaaagaaa tctttggtgt tattcctgac tttaacctta aagatggtgc acgccaggtt
    11521 tctgtaaccc gtccactgcc gtccctgccc ggcagggatg aggctgtccc ggccccctca
    11581 gaacaactga tgcgggtgtt cacgtggttt cagaaaaaac agttaacacc ggccattaat
    11641 gaaatagcca taccggagcc attaccaggc aacgatggtg aaccagcccc tgttcagaaa
    11701 tggaaagaat atcagttcag tttatcaact cctgtaaatc ctgatgagtt tttcccttta
    11761 cttcaggata ccggcgtccg tctgagcaat atccattttg aattaaacgg cggtactttc
    11821 agttattcct cagaaggtca tatatatgcg tccaagtaaa ttactcatta ttccttccat
    11881 atttttcttc tctggttttt catttgccac aacacaacct cttgtcacaa taggagaact
    11941 tgaagcacaa caaaaccgga atattctttt acaggcaaaa gttcagggtg cacagttaca
    12001 gaaacaactt gaagaatcag atgttacttc atcatctgaa actgtttcag gtttctctgg
    12061 tgtgaccgcc cccttgccat cagtttcaga gcaaacggca ccgaaaaaca gaaaagaatt
    12121 gcccatcatt atggaaataa acggcaaaga taaacgtctt catgccgttc ttcgcatggc
    12181 tgatggacgt cagacaagcg tcaccactgg cagccagctt ccaggtacat ccgtcacggt
    12241 gaagtcgatc tcattgtctg gcgtcacgtt aagtgatggc actaccctta ctttctgagt
    12301 aacgtgatat ggataaatat aatctgaagg atgctctcct ttttatatcc cggggagaca
    12361 cccatgaaat tcttatagaa acaaaccagc ggacaaggcc tgacgtacaa agtaatctcc
    12421 aggaactgct aaaattatat ccgggtatca acccaaaagt agtctcgttg tcggagttac
    12481 aggaagcagg tcaggatgaa aaaaacagag gcccgaaaga acgaattatt catctgaaag
    12541 atcttgctga tgtcagtgaa agtgagaaga aagttc
//
LOCUS       DAZPAC010000117        12561 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZPAC010000117 DAZPAC010000000
VERSION     DAZPAC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.117"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..70)
                     /locus_tag="ACHUPM_002721"
     CDS             complement(<1..70)
                     /locus_tag="ACHUPM_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870453.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLK"
     gene            689..1459
                     /locus_tag="ACHUPM_002722"
     CDS             689..1459
                     /locus_tag="ACHUPM_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR0870454.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1450..1923
                     /locus_tag="ACHUPM_002723"
     CDS             1450..1923
                     /locus_tag="ACHUPM_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR0870455.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2031..2570
                     /gene="dnaT"
                     /locus_tag="ACHUPM_002724"
     CDS             2031..2570
                     /gene="dnaT"
                     /locus_tag="ACHUPM_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR0870456.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2573..3310
                     /gene="dnaC"
                     /locus_tag="ACHUPM_002725"
     CDS             2573..3310
                     /gene="dnaC"
                     /locus_tag="ACHUPM_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR0870457.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3359..3853
                     /gene="yjjA"
                     /locus_tag="ACHUPM_002726"
     CDS             3359..3853
                     /gene="yjjA"
                     /locus_tag="ACHUPM_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR0870458.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4107..6398
                     /gene="opgB"
                     /locus_tag="ACHUPM_002727"
     CDS             4107..6398
                     /gene="opgB"
                     /locus_tag="ACHUPM_002727"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR0870459.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="ACHUPM_002728"
     CDS             complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="ACHUPM_002728"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR0870460.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7698..8612
                     /locus_tag="ACHUPM_002729"
     CDS             7698..8612
                     /locus_tag="ACHUPM_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR0870461.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8827..10178
                     /locus_tag="ACHUPM_002730"
                     /pseudo
     CDS             8827..10178
                     /locus_tag="ACHUPM_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10227..11882)
                     /gene="tsr"
                     /locus_tag="ACHUPM_002731"
                     /pseudo
     CDS             complement(10227..11882)
                     /gene="tsr"
                     /locus_tag="ACHUPM_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12001..12447)
                     /gene="hpaR"
                     /locus_tag="ACHUPM_002732"
     CDS             complement(12001..12447)
                     /gene="hpaR"
                     /locus_tag="ACHUPM_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR0870462.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         3099 a         3291 c         3011 g         3160 t
ORIGIN      
        1 cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt ttgcagcatc
       61 ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta ttcaggatat
      121 tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat ccttagcatt
      181 cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt cagaaggtta
      241 gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta cattaagttg
      301 ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt aaatgatatc
      361 ccttcccgca gaatgaaaaa tcacacaacc aatatttatt caatgaaatc tgatggtttt
      421 aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt aaaggccaaa
      481 caataaattc attatagtga taatattcat gtgatagttt gtttgcggcg agagataatt
      541 cgctttttat caccgagctt gtcccgataa ccaggattaa tcagcgcaat tttgatctta
      601 acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc tgaacaatac
      661 gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag ccgtaacacg
      721 gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg cgttggttga
      781 tgagctttcc tcacgactgg gtcgggcgtt ggggatggac agcgtcgaaa gttctatctc
      841 ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat cgacacgtaa
      901 aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca ttgtgattct
      961 ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc aaattcagcc
     1021 attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg cctgtttctg
     1081 caaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca gtacggccgc
     1141 gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccgcagatca acttttgcct
     1201 caccgctttc gccgccacca ccatttccgg attgcttttg caacttccca ctttcagcaa
     1261 tacacccact atcgcgatgg ccgccagcgt tctgctgctg gtgccgggat ttccgttgat
     1321 taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac gctgggcgat
     1381 cgccagtctg ctgacactgg ctacctgcgt cggcgtggtg atggcactga cgatttgggg
     1441 gctacgcgga tgggtgtgat cgagtttctg tttgcattgg cgcaggatat gatcctcgcc
     1501 gccattccag cggtcggctt tgcgatggtg ttcaacgttc ccgttcgggc attacgctgg
     1561 tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac cagcgggctg
     1621 aatattgagt ggtcaacctt tatggcctcc atgctggtcg gcaccattgg tattcaatgg
     1681 tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat ccctatgttc
     1741 ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca gttaggttac
     1801 agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc gattgttggt
     1861 gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg ccctcgcgta
     1921 taaaattgcc tcgcttaaag cacacggatg agagacagcc tcctctcctc cgtgtgttac
     1981 tataaaagtt atctcccttc tcgttcatcg ttccatattt gagaaacagt atgtcttcca
     2041 gagttttgac cccggacgtc gttggtattg acgccctggt acacgatcac caaaccgttc
     2101 tggcaaaagc tgaaggcggt gtggttgccg tatttgctaa caatgccccg gcgttttatg
     2161 ccgtcacgcc tgcacgcctg gctgaactgc tggcgctgga agaaaagctg gcgcgtccgg
     2221 gaagcgatgt cgctctggac gatcaactct atcaggaacc gcaagccgct cccgttgctg
     2281 tacccatggg gaaattcgcc atgtatccgg actggcaacc cgatgccgat tttatccgcc
     2341 tggcggcgct atggggtgtg gcactaagag agccggtgac caccgaagaa ctggcctcat
     2401 tcattgccta ctggcaggcg gaaggtaaag tctttcacca tgtgcagtgg caacaaaaac
     2461 tggcgcgcag cctgcaaatc ggtcgtgcca gcaacggcgg actgccgaaa cgagatgtga
     2521 atacggtcag cgaacctgac agtcaaattc caccaggatt cagagggtaa cgatgaaaaa
     2581 cgttggcgac ctgatgcaac gcctgcaaaa aatgatgcct gcccatatca aacctgcatt
     2641 caaaacgggt gaagaactac tggcctggca gaaagaacaa ggagcgatcc gctccgccgc
     2701 tctcgaacgt gaaaatcggg cgatgaaaat gcagcgcacc tttaaccgct ccggtattcg
     2761 gccactgcat cagaactgct cctttgagaa ctatcgcgtt gagtgtgaag ggcagatgaa
     2821 tgcgttaagc aaagcgcgcc agtacgtcga agagtttgac ggcaacatcg ccagcttcat
     2881 cttttctggt aagccaggaa ccggcaaaaa ccatctggcg gcggcaatct gcaacgagct
     2941 gctgctacgc ggtaaatccg tattgatcat caccgtggcc gatattatgt cggcgatgaa
     3001 agataccttc aggaatagcg gtaccagtga agaacaactg cttaacgatc tgagcaacgt
     3061 tgatctgctg gtgatcgatg agatcggcgt gcagaccgaa tcgaaatacg aaaaagtgat
     3121 catcaaccag atcgtcgatc gccgctcttc ttccaaacgc ccaaccggga tgctgaccaa
     3181 cagtaatatg gaagagatga ccaagttgct gggcgaacgc gtgatggacc gtatgcgcct
     3241 gggtaacagt ttgtgggtga tcttcaactg ggatagctac cgtagccggg taacaggtaa
     3301 agagtattaa tccccgtttc tggcggtata ctgcgacaca attccacctt gagtaatgat
     3361 gatgaaaact gttaaacatc ttctgtgctg tgccattgcc gccagcgcat taatttccac
     3421 cggggtgcat gctgcgtcct ggaaagatgc gctctccagc gccgccagcg aacttggcaa
     3481 ccaaaacagc acgacacagg aaggcggttg gtcgctcgcg tcattaacta acttgcttag
     3541 cagcgggaac caggccttaa gcgcagataa catgaacaac gccgcaggca ttctgcaata
     3601 ctgcgcgaag caaaagctgg cgtcggtaac cgatgccgaa aacatcaaga accaggtgct
     3661 ggaaaagctg ggcctgaaca gtgaagagca aaaagaagac accaactatc tggatggcat
     3721 tcagggtttg ctgaaaacca aagatggtca gcaactcaat ctggataaca tcggaacgac
     3781 tccgctggca gaaaaggtga aaaccaaagc ctgcgatctg gtgttaaagc aggggctgaa
     3841 cttcatttcc tgaaccgcga aacgccgcgt tttagctgtt acccggctga cgcggctctc
     3901 tcctgccctt ttccctgctc ctggacggtt ttccccctgt tacacgggaa gcgaccgcga
     3961 tcaaaaaagt ctgtttataa actaattcct aacaacatcg catttttttg acactagaat
     4021 tgcagcaata tggcgacgcc attacattgt gcttctcaaa aagcttaaac gttggctaaa
     4081 cggcccggtt tagtgaggat cgcccgttgt cagaactact ctctttcgcc ctttttctcg
     4141 cctctgtgct gatttacgca tggaaagcgg gacgtaacac ctggtggttt gcagccacat
     4201 taacggtgct ggggctattt gtcgttttaa atatcaccct gtttgccagc gactatttta
     4261 ctggcgatgg tattaacgac gcggttctct ataccttaac caacagcctg accggtgctg
     4321 gcgtcagcaa atacatcctg ccgggtatcg gcattgtgct ggggctgaca gcggtgttcg
     4381 gtgcgctggg ctggatcctg cgccgtcgtc gccatcatcc gcaccatttt ggttacagcc
     4441 tgctggcgct cttactggcg ctgggttcgg tggacgccag cccggcattt cgtcagataa
     4501 cggaactggt gaaatcccag tcacgcgatg gcgacccgga ctttgcggct tattataaag
     4561 agccgtcgaa aactatccct gacccgaaac tcaacctggt ttatatctac ggcgaaagtc
     4621 tcgagcggac ctattttgat aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt
     4681 tgaaaaatga aggcctggat ttcagccaca cgcagcagct gccaggaacg gattacacga
     4741 ttgcgggcat ggtggcttct cagtgcggca taccgctgtt tgcccccttt gaaggcaacg
     4801 cctccgcctc tgtctccagc ttcttcccgc agaacatctg tctgggcgat atcctgaaaa
     4861 actcgggtta tcagaactat ttcgtgcagg gcgcgaatct gcgttttgcc ggtaaagatg
     4921 tgttcctgaa gtcgcatggc ttcgaccact tatacggctc agaagagctg aaaagcgtgg
     4981 tggccgaccc gcactatcgc aacgactggg gattctacga cgataccgtt ctcgatgaag
     5041 cgtggaaaaa gtttgaagag ctttcccgct caggtcagcg attctcactg tttaccctga
     5101 cagtcgatac ccatcacccg gatggtttta tctcgcgtac ctgtaaccgc aaaaaatatg
     5161 attttgacgg taagccgaac cagtcattca gcgcggtaag ttgcagccag gagaatatcg
     5221 cggcgtttat caacaaaatc aaagcgtcac cgtggtttaa agataccgtc atcgtcgtct
     5281 cttctgacca tttagcgatg aacaacacgg cgtggaaata cctcaataag caggatcgca
     5341 ataacctgtt ttttgtcatt cgtggcgaca agccgcagca agagacgctg gcagtgaagc
     5401 gtaacacgat ggataacggc gcgacggtgc tggacattct cggtggcgat aactatctcg
     5461 gacttggtcg tagcagttta tccgggcagt cgatgtcgga aatcttcctc aatatcaaag
     5521 agaaaacatt ggcgtggaag ccggatatca tccgcctgtg gaaattccct aaagagatga
     5581 aagagttcac catcgaccag cagaaaaaca tgattgcctt ctcgggtagc catttccgtt
     5641 tgccgctgct gttgcgggtt tcagacaaac gcgtggaacc gctgccggaa agcgaatact
     5701 cagcaccgct gcgtttccag ctggccgatt tcgctccacg cgacaatttc gtctgggttg
     5761 accgttgcta taagatggca caactctggg ctccggaact ggcactctcc accgactggt
     5821 gtgtctcgca agggcagctt ggcggtcagc aaattgttca gcatgttgac aaaacaacat
     5881 ggcagggcaa aacggcattt aaagatacgg tcatcgacat ggcgcgttat aaaggcaatg
     5941 tcgatacgct gaaaattgtt gataacgata ttcgctacaa agccgacagt ttcatcttca
     6001 acgtcgccgg tgcgccagaa gaggtgaaac agtttagtgg gatttcccgt ccagagtcgt
     6061 ggggccgctg gtccaacgcg cagctgggcg atgaagtaaa aatcgagtac aagcatccgc
     6121 tgccaaagaa atttgacctg gtgattaccg ccaaagcata cggcaataac gccagccgtc
     6181 ctattccggt acgcgtaggc aatgaagaac aaacccttgt gctgggcaat gaagtgacca
     6241 ccaccacact gcatttcgat aacccaaccg atgccgacac actggtaatt gtgccgccgg
     6301 aacctgtctc aaccaacgaa gggaatatcc tcggacactc gccgcgtaag ctcgggatcg
     6361 gcatggtgga aattaaagtg gtggaacgtg aaggataatt cctgactgat gtgtaggtcg
     6421 cataaggtgc gacctacaca ccttctgcgg cctacaaaac atcattgaga aacagaaaaa
     6481 aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc gccttatcag
     6541 aaagtaatta cacccttaat caactcacga ttgttaatca catcgcgctc gtaggtttct
     6601 gccagcgtgg cgaacggata gcgatgggtt aacatcatgt cagcagtgat ttttccttcc
     6661 gccatcagtc gaccgacttt agcaaaatct tccggcgtgg cgttgcggct acccatcatc
     6721 gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt aaacaggccg
     6781 acaaatacca ccgtgccgcc gtgacgaatc aaattcacgg tgttattcat cgcatgttga
     6841 ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc ccgtagctgc
     6901 gcgtcaaaat cttcggctga cgggtccagt aaaggtaatt ccagacgcgt tgccacatgt
     6961 tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatcggc tttagcgatt
     7021 gccgccgcgc ccagaccgat tggccccgcc ccgaccacca gcacctgctc gccgggagca
     7081 atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa tgctgccgcc
     7141 tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa atactcacta
     7201 aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt ggtacgcccg
     7261 cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac ttgctgacca
     7321 tttttaagat cagcaatatt tttacccagc ccaacaatct ccccacatat ttcatggcct
     7381 aaaacacgtg gataactaaa aaatggttga tttccacccc aggcatgaat atcggtaccg
     7441 caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat aggtatctcg
     7501 cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat cgtagacatg
     7561 attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat ggcagcggaa
     7621 cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg ttttaatgga
     7681 ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg attaaccagg
     7741 tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa agtgcgctgg
     7801 cggaaatgta caacatcagc cgcaccacag tgcgtcatat tctcagccac ttacgcgaat
     7861 gcggcgtctt gacgcaggtt ggaaacgact atgttattgc gcgtaaacct gaccatgatg
     7921 acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt gagcaggctt
     7981 ttttcacgat gataaaccag cgccagttac gcccagggga aacgttttct gaactgcaac
     8041 tggctcgggc cgcgggtgtc agcccggttg tagtaagaga atatctttta aaattcggcc
     8101 gttataatct tattcacagc gaaaagcgcg gccagtggag catgaagcaa ttcgatcaat
     8161 cctacgccga gcagctgttt gagctacgcg aaatgctcga aacacattca cttcagcact
     8221 ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg ctcgaacgtc
     8281 atcggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa cttgacagag
     8341 attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa tcgcttgaga
     8401 tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt aaacagcgca
     8461 atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc cgcagtgacc
     8521 ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc taaacaatca atgattcgat
     8581 ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc aaactattag
     8641 cgggtgcacg gttttatcaa aagataaata ccgggaaatt cccgcttacc tatgctcaca
     8701 atcagtcgac taaaaatgtt tttgtgagcg gtagtaaagt cctaaaactt taacctgccg
     8761 ttaatgtcaa ccttgcgtaa ttcacaatta cgtaaacaat taaaattata aatacaggag
     8821 tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt cttcatttac tccatcttcg
     8881 tcggcagata ttcccgtgcc accagatgga ttagttcaac gcagtacccg aattaaacgc
     8941 attcaaacca ccgccatgtt gttattattt tttgcggcgg taatcaatta tctcgaccgc
     9001 agttcgctgt cggtagcaaa tttaacgatt cgtgaagaat tgggattaag tgccaccgaa
     9061 atcggcgctt tgctctccgt gttttcactc gcttacggga ttgcgcaact tccttgcggc
     9121 ccactattgg atcgtaaagg cccacgcctg atgctgggac tggggatgtt cttctggtca
     9181 ctgttccagg caatgtctgg catggtgcac aactttacgc agttcgtgtt ggtgcgtatc
     9241 ggtatgggga ttggtgaagc gccgatgaac ccatgcggtg taaaagtcat taacgactgg
     9301 ttcaacatca aagagcgcgg acgcccgatg ggcttcttca acgcagcttc taccattggc
     9361 gttgccgtaa gcccaccgat tctggcggcg atgatgctgg tgatgggctg gcgcgggatg
     9421 tttattacca ttggtgtact ggggattttt ctcgccatcg gctggtatat gctctatcgc
     9481 aaccgcgagc acgtagaact gactgccgtt gaacaagctt atctcaatgc aggtagcgtc
     9541 aatgcccgcc gagatccgct cagttttgcc gaatggcgca gcctgttccg taaccgtaca
     9601 atgtggggaa tgatgctcgg attcagtggc atcaactaca ctgcgtggct gtatctggcc
     9661 tggcttcctg gttacctgca aacagcctat aacctggatt taaaaagcac agggttgatg
     9721 gcggctatcc ctttcctgtt tggggctgcc gggatgctgg tcaacggtta cgttactgac
     9781 tggctggtca aagggggaat ggctccgatt aaaagccgta agatctgcat tattgccggg
     9841 atgttctgtt ctgccgcctt tacgctggtc gtaccgcaag cgacaacatc catgacagcg
     9901 gttctgctga ttggcatggc actgttctgt attcaacatc ctgctggggc ttgatccacg
     9961 tcgcagttgc ttctcgcatg actgcgtcgg tgggcagtat ccagaacttt gccagcttca
    10021 tctgcgcctc ttttgcgccg atcattactg gttttattgt tgataccacc cactcattcc
    10081 gtctggcact aatcatctgc ggttgcgtca ccgcagcggg ggcactggcg tacatcttcc
    10141 tggttcgtca gccgatcaac gacccacgta aagattaatc tccttatgcc cgataacatt
    10201 ttgcttatcg ggcattttca tggcgattaa aatgtttccc agttctcctc gctatctgcc
    10261 acggccattt tacgcggcgc agctggcgtc acggttttta ccacagccga tgtttcacgc
    10321 tgctgttgct gaatccggaa cactgccact gcttcggtca ggcgactggc ctgctcttcc
    10381 agcgcggcgg cggcagcggc agactcttcc accagcgcgg cgttctgttg agttacccgg
    10441 tccatctcag caaccgctaa gccaacctga tcgataccac ggctctgctc atcagaagca
    10501 gaagcaattt cgcccataat gtccgtcacg cgggtcacgg cgctgacaat ctccgccatt
    10561 gtttccccgg cgctttcgac cagcgtagag ccaacatcca ctttccccac cgagtcttca
    10621 atcaggcttt taatttcacg agccgcctgg gcgctgcgct gggccagatt acgcacttct
    10681 cccgcgacca ccgcaaaacc gcgcccttgc tcacccgcac gcgcagcctc aaccgccgcg
    10741 ttcaaagcca gaatattggt ctggaaggca atgccgtcaa ttacgctgat aatatcggcg
    10801 attttctgcg aactggtgga gatatcgcgc atagtctgca ccacgttatc taccactttg
    10861 ccgccgcgtt gcgccgtttc agaagcactt aacgccagat ggctggcctg gcgcgcattt
    10921 tcggcgttct gcttcaccgt tgcggtcagt tactccatgc tggctgccgt ctcttccagc
    10981 gaagcggcct gttgctcggt gcgcgaagag agatcgttat tgccggtggc gatttcgctg
    11041 gcaccgctat agatggcatt ggccccgttg cgcacatcac cgacggtacg catcagctct
    11101 ccctgcatat ggcgcaaact ctctgccagt tgccccatct cattagagcc atccacctca
    11161 atcggtttca ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag gcgattcatt
    11221 ggcgctacca gcgaggcttt aataccgaac cagacggcga agatgaccgc cagtacgacg
    11281 atcatcacgc ccaccagaat ccacatcgcc tggctgtagg aggcattgtt atcgctgacg
    11341 gcgatatcat agagccgatc gttttgctcc atgtaagcca catactgctt ctcgaaaccg
    11401 tcctgatatc cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc acctaacagt
    11461 tggatcagct ccgccagcgc attgtgataa atatcgtaat tacgtttgat ctctgccgct
    11521 gcggcggtgc tctgacgcgg gtcacgcggc aacgcttcgt aatccgccca gtttttttcc
    11581 gcctgtttca gcgaaatact ggcactctgc atcagctcag caacggttga accgctacca
    11641 atattgttct gatccatcat gtagcggata cccgcgcggt tgagggtgtt acgcgtctgc
    11701 aacaacgcga cccagctgcc attcagcgtg gattgctgct ggcgaatggt ttgtaaaaca
    11761 gtgaaatttt ctttgtcatt ctttaaggca ttaaagaaca gaccgcctga tgtcagttgt
    11821 aaaaggccaa aaacggccaa aaccagcagt aagctggtca cgatcttgat acgttttaac
    11881 atggtttctc tttcctgtgg accgatgcaa gattttcggc ctggaaagca aaaactttat
    11941 gaaaaggtgt aacccaccat ccatggaaaa accaggcgct ggttatcaga tactaaaaag
    12001 ttattcatta tcgcccggga tggcttcttg tcgggaattg cccagagcaa taaattcctc
    12061 taacagatgg gttaactgtt gcattttctc ggcagtaaac tgagcttcaa tctgccgata
    12121 ggcttcttca atctgtgtct gagcgcggtt atacagcgcc tgcccctctt tggttagcga
    12181 gatatagagc ttccgctgat cattgatcgg ctttaatcgc aacactaaac cgtcgcgttc
    12241 catccgcgtg aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat acgccagatc
    12301 gtgaaaatcc attgatgggc tttccgccag aatacgcacg atgcgccact gttgctcagt
    12361 caaattatgc cgtttaacga ttgggcgaaa ataactcatc gccgcttctc gcgcctggag
    12421 caacgcaatg gttagtgagt cgtgcattat ctttcccctg aaaaattcaa aatcattaat
    12481 atagaaacag ttaatcgtca cgttaccggg actgcgctgc ccctgcaatt cctgatttta
    12541 tcaaaattga tgatatttta t
//
LOCUS       DAZPAC010000118        12359 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZPAC010000118 DAZPAC010000000
VERSION     DAZPAC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.118"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..330)
                     /locus_tag="ACHUPM_002733"
     CDS             complement(<1..330)
                     /locus_tag="ACHUPM_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFR0870463.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIYMNASRLQGIHCFDMTGRLQLYIRLYCSRNAA
                     SPYVIRRL"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHUPM_002734"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHUPM_002734"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR0870464.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHUPM_002735"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHUPM_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR0870465.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHUPM_002736"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHUPM_002736"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR0870466.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHUPM_002737"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHUPM_002737"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR0870467.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLMDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHUPM_002738"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHUPM_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR0870468.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHUPM_002739"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHUPM_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR0870469.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHUPM_002740"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHUPM_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR0870470.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHUPM_002741"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHUPM_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR0870471.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHUPM_002742"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHUPM_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR0870472.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHUPM_002743"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHUPM_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR0870473.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHUPM_002744"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHUPM_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR0870474.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHUPM_002745"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHUPM_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR0870475.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         2831 a         3451 c         3021 g         3056 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagta acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg atgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccatcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag tacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat gacacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaact
//
LOCUS       DAZPAC010000119        12116 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZPAC010000119 DAZPAC010000000
VERSION     DAZPAC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12116
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.119"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            53..352
                     /locus_tag="ACHUPM_002746"
     CDS             53..352
                     /locus_tag="ACHUPM_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR0870476.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(472..651)
                     /locus_tag="ACHUPM_002747"
     CDS             complement(472..651)
                     /locus_tag="ACHUPM_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR0870477.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            725..2086
                     /gene="pabB"
                     /locus_tag="ACHUPM_002748"
     CDS             725..2086
                     /gene="pabB"
                     /locus_tag="ACHUPM_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR0870478.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2090..2668
                     /locus_tag="ACHUPM_002749"
     CDS             2090..2668
                     /locus_tag="ACHUPM_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR0870479.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHUPM_002750"
     CDS             2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHUPM_002750"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0870480.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4347..5945
                     /locus_tag="ACHUPM_002751"
     CDS             4347..5945
                     /locus_tag="ACHUPM_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR0870481.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5949..7499)
                     /gene="yoaE"
                     /locus_tag="ACHUPM_002752"
     CDS             complement(5949..7499)
                     /gene="yoaE"
                     /locus_tag="ACHUPM_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR0870482.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(7487..7645)
                     /gene="yoaL"
                     /locus_tag="ACHUPM_002753"
     CDS             complement(7487..7645)
                     /gene="yoaL"
                     /locus_tag="ACHUPM_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR0870483.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7962..8933
                     /gene="manX"
                     /locus_tag="ACHUPM_002754"
     CDS             7962..8933
                     /gene="manX"
                     /locus_tag="ACHUPM_002754"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR0870484.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8996..9796
                     /gene="manY"
                     /locus_tag="ACHUPM_002755"
     CDS             8996..9796
                     /gene="manY"
                     /locus_tag="ACHUPM_002755"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR0870485.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9809..10660
                     /gene="manZ"
                     /locus_tag="ACHUPM_002756"
     CDS             9809..10660
                     /gene="manZ"
                     /locus_tag="ACHUPM_002756"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR0870486.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10715..11173
                     /locus_tag="ACHUPM_002757"
     CDS             10715..11173
                     /locus_tag="ACHUPM_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR0870487.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            11602..>12116
                     /gene="mntP"
                     /locus_tag="ACHUPM_002758"
     CDS             11602..>12116
                     /gene="mntP"
                     /locus_tag="ACHUPM_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR0870488.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGL"
BASE COUNT         2971 a         3014 c         3050 g         3081 t
ORIGIN      
        1 taaggtcata tatagcctta tcgataacat ggttaatttt aaggataaga atatgcctgc
       61 tgtaatagat aaagccctgg atttcattgg tgccatggat gtatcagcgc caacaccaag
      121 ttcgatgaat gaaagcacgg cgaagggaat ctttaaatat ttaaaagaac tgggagtacc
      181 tgcgagtgcc gctgatatta cggcgcgagc tgaccaggaa ggctggaacc ctgggtttac
      241 ggaaaaaatg gttggatggg caaaaaaaat ggagtcaggt gaacgctctg tgattaaaaa
      301 tcctgaatac ttttcaacat atatgcagga agaactaaaa gcactggtct gagtaaaatt
      361 tacatcagca taaatggatc aggacgcttt taatcacata taaaaagcgt cctttttctc
      421 acaatcaaat aatcatcggc ttgtataacg aaatcaatac ggattagtgt gttactcgtc
      481 ttcatcctca aaacgcgcca cgattcgctc gccggaatgg ttggcacgca gctcttctgc
      541 caccaacgcg attgcctggc cgctgctcat cccttgtgcc atcagttcct ggatccgctc
      601 gacagctttt tgctgctgtt catgggtgag tgaaggtaaa cctgcaaaca ttgttaactc
      661 ctgctaaatt gttggcgcta attatttcat gctacccggc acatagccag tagagtcagg
      721 actgatgaag acgttatctc ccgctgtgat tactttaccc tggcgtcagg acgccgctga
      781 attttatttc tcccgcttaa gccacctgcc gtgggcgatg cttttacact ccggctatgc
      841 cgatcatccg tatagccgct ttgatattgt ggtcgccgat ccgatttgca ctttaaccac
      901 tttaggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc acaacgacca ctgatgaccc
      961 gctacaggtg ctccagcagg tgctggatcg cgcagacatt cgcccaacgc ataacgaaga
     1021 tttgccattt cagggcggcg cactggggtt gtttggctac gatctgggcc gccgttttga
     1081 gtcactgcca gaaattgcgg aacaagatat cgttctgccg gatatggcag tgggtatcta
     1141 cgattgggcg ctcattgtcg accaccagcg tcatacagtt tctttgctga gtcataatga
     1201 tgtcaatgcc cgtcgggcct ggctggaaag ccagcaattc tcgccgcagg aagatttcac
     1261 gcttacttcc gactggcaat ccaatatgac ccgcgagcag tacggcgaaa aatttcgcca
     1321 ggtacaggaa tatctgcaca gcggtgattg ctatcaggta aatctcgccc agcgttttca
     1381 tgcgacctat tctggcgatg aatggcaggc attccttcag cttaatcagg ccaaccgcgc
     1441 gccatttagc gcttttttac gtcttgaaca gggtgcaatt ttaagccttt cgccagagcg
     1501 gtttattctt tgtgataata gtgaaatcca gacccgccct attaaaggca cgctaccacg
     1561 cctgcccgat cctcaggaag atagcaaaca agcagaaaaa ctggcgaact cagcgaaaga
     1621 tcgtgccgaa aatctgatga ttgtcgattt aatgcgtaat gatatcggtc gtgttgccgt
     1681 agcaggttcg gtaaaagtac cagagctgtt cgtggtggaa cccttccctg ccgtgcatca
     1741 tctggtcagc accataacgg cacaactacc agaacagtta cacgccagcg atctgctgcg
     1801 cgcagctttt cctggtggct caataaccgg ggctccgaaa gtacgggcta tggaaattat
     1861 cgacgaactg gaaccgcagc gacgtaatgc ctggtgcggc agcattggct atttgagctt
     1921 ttgcggcaac atggatacca gcattactat ccgcactctg actgccatta acggacaaat
     1981 atactgctct gcgggcggtg gaattgtcgc cgatagccag gaagaagcgg aatatcagga
     2041 aacttttgat aaagttaata agatattacg ccaactggag aagtaagacg tggaataccg
     2101 tagcctgatg cttgatgatt ttttatcgcg ctttcaactt ttgcgcccgc aaattaaccg
     2161 ggaaacccta aatcatcgtc aggctgccgt gttaatcccc atcgtccgtc gaccgcaacc
     2221 ggggttgttg ctgactcagc gttcaattca tctgcgtaaa cacgctggac aagtggcatt
     2281 ccctggaggt gcagtcgatg acacggacgc atcagttatc gccgccgcgc tgcgcgaagc
     2341 tgaagaagag gtcgctatac cgccttccgc cgttgaagtt atcggcgtgc tgccgcccgt
     2401 cgatagcgta actggctatc aggtaacccc agtggtcggc attatcccgc ccgatctgcc
     2461 gtatcgcgcc agtgaagatg aagtctcggc ggtgtttgaa atgccgctcg cccaggcatt
     2521 acatctgggt cgttatcacc ctttagatat ctaccgccgt ggcgattcac atcgggtatg
     2581 gctgtcctgg tacgaacagt attttgtatg gggaatgacc gcaggcataa ttcgtgagct
     2641 ggcgctgcaa attggtgtga aaccctgact atacttatct ttacatctac aaaacactac
     2701 ttgagacaat catcgcaata ttagttaaat cgcggttttt gattagttta attcatgtga
     2761 atagttaagc cagtcgccgc gttccctctt acactatgcg ctgttattag ttcgttactg
     2821 gaagtccagt caccttgtca ggagtattat cgtgattagt ctattcgaca tgtttaaggt
     2881 ggggattggt ccctcatctt cccataccgt agggcctatg aaggcaggta aacagttcgt
     2941 cgatgatctg gtcgaaaaag gcttactgga tagcgttact cgcgttgccg tggacgttta
     3001 tggttcactg tcgctgacgg gtaaaggcca ccacaccgat atcgccatta ttatgggtct
     3061 tgcaggtaac gaacctgcca ccgtggatat cgacagtatt cccggtttta ttcgcgacgt
     3121 agaagagcgc gaacgtctgc tgctggcaca gggacggcat gaagtggatt tcccgcgcga
     3181 caacgggatg cgttttcata acggcaacct gccgctgcat gaaaacggta tgcaaatcca
     3241 cgcctataac ggcgatgaag tcgtctacag caaaacttat tattccatcg gcggcggttt
     3301 tatcgtcgat gaagaacact ttggtcagga tgctgccaac gaagtgagcg tgccgtatcc
     3361 gttcaaatct gccaccgaac tgctcgcgta ctgtaatgaa accggctatt cgctgtctgg
     3421 tctcgctatg cagaacgaac tggcgctgca cagcaagaaa gagatcgacg agtatttcgc
     3481 gcatgtctgg caaaccatgc aggcatgtat cgatcgcggg atgaacaccg aaggtgtact
     3541 gccaggcccg ctgcgcgtgc cacgtcgtgc gtctgccctg cgccggatgt tagtttccag
     3601 cgataaactg tctaacgatc cgatgaatgt cattgactgg gtaaacatgt ttgcgctggc
     3661 agttaacgaa gaaaacgccg ccggtggtcg tgtggtaact gcgccaacca acggtgcctg
     3721 cggtatcgtt ccggcagtgc tggcttacta tgaccacttt attgaatcgg tcagcccgga
     3781 catctatacc cgttacttta tggcagcggg cgcgattggt gcattgtata aaatgaacgc
     3841 ctctatttcc ggtgcggaag ttggttgcca gggcgaagtg ggtgttgcct gttcaatggc
     3901 tgctgcgggt cttgcagaac tgctgggcgg tagcccggaa caggtttgcg tggcggcgga
     3961 aattggcatg gaacacaacc ttggtttaac ctgcgacccg gttgcagggc aggttcaggt
     4021 gccgtgcatt gagcgtaatg ccattgcctc tgtgaaggcg attaacgccg cacggatggc
     4081 tctgcgccgc accagtgcac cgcgcgtctc gctggataag gtcatcgaaa cgatgtacga
     4141 aactggtaag gacatgaacg ccaaataccg cgaaacctca cgcggtggtc tggcaatcaa
     4201 agtccagtgt gactaatact tcttactcgc ccatctgcaa cggatgggcg aatttatacc
     4261 cgctttctcg tctgctgtaa tattccccac tacacttcca ctgttgcgtc aggcgtttgt
     4321 cgccatacgc ttacagggtg gcccgcatgc aaaaagcaca acggatcatt aaaacctatc
     4381 gccgtaatcg aatgattgtt tgtacgattt gcgcactcgt tacgctcgct tcgaccctga
     4441 gcgtgcgatt tatttcacag cgtaacttaa atcaacaacg ggtagtacaa ttcgccaatc
     4501 acgctgtaga ggaattagat aaagtactgc ttcccctaca ggcaggtagc gaagtcttgc
     4561 ttccgctgat tggtctgccc tgctctgtcg cccatttgcc attacgtaaa caggcggcaa
     4621 aactccaaac tgtgcgatcc attggcctgg tgcaagacgg aacactctat tgctccagca
     4681 tttttggtta tcgcaatgtg cccgtcgtgg acattctggc tgaacttcct gcaccgcaac
     4741 cacttttacg cctgacgatc gaccgtgccc tgattaaagg cagtccggtt ttgattcaat
     4801 ggacgcctgc agcgggcagt agcaatgctg gggtcatgga gatgattaac atcgacttac
     4861 tgacggcaat gctccttgag ccacaactgc cgcaaatcag tagcgccagc ctgacggtgg
     4921 acaaacggca tttgctctat ggtaatgggc tggtagattc ccttccgcaa cctgaagaca
     4981 atgaaaacta tcaggtttct tcgcaacgct ttccttttac cattaacgtt aatggtccgg
     5041 gggctacggc gctggcatgg cactatcttc caacacaatt accgctggcg gtgctgctaa
     5101 gtttactggt gggctacatc gcctggctgg cgaccgctta ccggatgagc ttttcccgcg
     5161 aaatcaatct gggcctggcg caacatgagt tcgaattgtt ctgtcagcct ttgcttaatg
     5221 cgcgcagcca gcaatgtatt ggtgtagaga ttttgctgcg ctggaacaat ccgcgtcagg
     5281 gctggatttc accggatgtg tttattccta tcgcggaaga acatcattta attgtgccac
     5341 tgacccgcta tgtgatggca gaaaccattc gtcagcgcca tgttttcccg atgagtagtc
     5401 agtttcatgt tggcattaac gtcgcaccca gccattttcg ccgtggtgtg ctgataaaag
     5461 atctcaatca gtactggttt agcgctcacc cgattcagca actgatcctc gaaatcaccg
     5521 aacgcgatgc cttactggat gttgattatc ggattgcccg cgagctacat cgtaaaaacg
     5581 tcaaactggc gattgatgac ttcggcaccg gcaacagttc attttcctgg cttgaaacat
     5641 tacgtcctga catgctgaaa attgataagt catttaccgc agctataggt tctgacgcgg
     5701 ttaactcgac ggtgaccgat atcatcatcg cgctggggca aagactgaat attgaactgg
     5761 tggcggaggg cgtggaaacg caagaacagg cgaagtattt gcgccgtcat ggcgtgcata
     5821 ttttgcaagg gtatttgtac gcacagccga tgccgctacg tgattttccc aaatggctgg
     5881 cgggcagcca accgccgccc gcccggcata atggacatat cacgcccatt atgccgttac
     5941 gttaatgctt actcatcttc atcgtgcgcc ggttgctctt taacaatgcg aaccagatca
     6001 acacgataat cattggcttc aatgatggtg atatgcagtg gccctacatc aatcacatcg
     6061 cccacacggg gaatgtgacc atttgccgag atcacgaggc ccgcgaccgt cgcgatatca
     6121 tcgtcatcgg caaggtgctc aacatcaagc gcctgctgca aggcatgcaa atctgtaccg
     6181 ccttttacca gccagccgtc accatcagta atgatttccg gcgtttcgtc agcgtccggg
     6241 aattcacccg caatggcttc cagcacatcc agcggcgtga ccagaccttg taccacacca
     6301 aactcgttgg tcacgataac aaagctcccg cgagcacgac gcagcacgcc caacaggttg
     6361 atcggatcga gggtttccgg gacgataatc gccggagacg ccgaagcaat cgccgccaca
     6421 tcaacgccct cttccagcgc caccagcagt tctttagcac gtacaatacc gatgatttca
     6481 tccagttcac cgcgacatac cgggaacaga ctgtgcggtg aagagagcag ttgctcgcgg
     6541 atttcatcga ccccgagatt agcgtcaacc cagcttattt caccgcgcgg cgtcatgatc
     6601 ccgcgcagag aacgcgacgc aagcgtcagt acgccgttaa tcatgtaacg ttcttcttcg
     6661 gcaaatgcac cttccgggat cggcatcggg ttatcggcat cgtgctgaac attggcctga
     6721 cgtttcccgc ccatcaaacg caggatggca tcggcagtac gcgctcgcag cggcaaagtc
     6781 gactggtggc gaataaagtt gcgacgcgca atctggttaa acacttcgat gatgatcgag
     6841 aagccaatcg cggcatacag gtaacctttc ggaatgtgga aaccgaaacc ttctgccacc
     6901 agactcagac caatcattaa caggaagctc agacagagca ccaccaccgt ggggtgctgg
     6961 ttaacgaatc gcgtcagcgg tttggatgcc agcaacataa ccgccatcgc aatcactacc
     7021 gccgccatca tcaccggcag atggttaacc atccctactg cagtaattac cgcatccaac
     7081 gagaagacgg cgtcaaggat gacaatctgt gtgacgacca cccagaaact ggcgtagcct
     7141 ttaccgtggc cggaatcatg atcgcggttt tccagccgtt catgcagttc ggttgttgct
     7201 ttgaacagca agaatatccc cccgaacaac ataatcaggt cgcgtccgga gaaggagaaa
     7261 tccatgacgg taaatagcgg tttggtcagc gtgaccatcc atgaaatcag cgacagcagc
     7321 cccagacgca taatcagcgc cagtgataac cccagcaaac gcgctttatc gcgttgtttt
     7381 ggcggcagtt tgtcagcaag aatggcgatg aagaccaggt tatcgatacc cagcacaatt
     7441 tcgagaacaa caagcgtgag tagccccgcc caaattgagg ggtccattaa gaattccatg
     7501 acaagctcct gcttaaggaa tagctattcg acgccagaaa taatgcaggc gtaacgacaa
     7561 aatgcaaacg aaaggtgcgg catagagtgc cagaaaggca ggcgttaaaa ggcctgatgc
     7621 tgaaatgacg tcggtgacga tccatactgc gggctactgc cctatactcc atggttgtta
     7681 aacgggagtt aaacatatca gagacgcctc tgatttggca aagatttacc ttcctttgca
     7741 aacgaatgtg acaaggatat tttacctttc gaaatttctg ctaatcgaaa gttaaattac
     7801 ggatcttcat cacataaaat aattttttcg atatctaaaa taaatcgcga aacgcagggg
     7861 tttttggttg tagcccttat ctgaatcgat tcgattgtgg acgacgattc aaaaatacat
     7921 ctggcacgtt gaggtgttaa cgataataaa ggaggtagca agtgaccatt gctattgtta
     7981 taggcacaca tggttgggct gcagagcagt tgcttaaaac ggcagaaatg ctgttaggcg
     8041 agcaggaaaa cgtcggctgg atcgatttcg ttccaggtga aaatgccgaa acgctgattg
     8101 aaaagtacaa cgctcagttg gcaaaactcg acaccactaa aggcgtgctg tttctcgttg
     8161 atacatgggg aggcagcccg ttcaatgctg ccagccgcat tgtcgtcgac aaagagcatt
     8221 atgaagtcat tgcaggcgtt aacattccaa tgctcgtgga aacgttaatg gctcgtgatg
     8281 atgacccaag ctttgatgaa ctggttgcgc tggcagtaga aacaggccgt gaaggcgtga
     8341 aagcactgaa agccaaaccg gttgaaaaag ccgcgccagc acccgctgcc gcagcaccaa
     8401 aagcggctcc aactccggca aaaccaatgg ggccaaacga ctacatggtt attggccttg
     8461 cgcgtatcga cgaccgtctg attcacggtc aggtcgccac ccgctggacc aaagaaacca
     8521 atgtctcccg tattattgtt gttagtgatg aagtggctgc ggataccgtt cgtaagacac
     8581 tgctcaccca ggttgcacct ccgggcgtaa cagcacacgt agttgatgtt gccaaaatga
     8641 ttcgcgtcta caacaacccg aaatatgctg gcgaacgcgt aatgctgtta tttaccaacc
     8701 caacagatgt agagcgtctc gttgaaggcg gcgtgaaaat cacctctgtt aacgtcggtg
     8761 gtatggcatt ccgtcagggt aaaacccagg tgaataacgc ggtttcggtt gatgaaaaag
     8821 atatcgaggc gttcaagaaa ctgaatgcgc gcggtattga gctggaagtc cgtaaggttt
     8881 ccaccgatcc gaaactgaaa atgatggatc tgatcagcaa aatcgataag taacgtattg
     8941 tgttgattat cactcagttt tcacacttaa gtcttacgta aacaggagaa gtacaatgga
     9001 gattaccact cttcaaattg tgctggtatt tatcgtagcc tgtatcgcag gtatgggatc
     9061 aatcctcgat gaatttcagt ttcaccgtcc gctaatcgcg tgtaccctgg tgggtatcgt
     9121 tcttggggat atgaaaaccg gtattattat cggtggtacg ctggaaatga tcgcgctggg
     9181 ctggatgaac atcggtgctg cagttgcgcc tgacgccgct ctggcttcta tcatttctac
     9241 cattctggtt atcgcaggtc atcagagcat tggtgcaggt atcgcactgg caatccctct
     9301 ggccgctgcg ggccaggtac tgaccatcat cgttcgtact attaccgttg ctttccagca
     9361 cgctgcggat aaggctgctg ataacggcaa cctgacagcg atttcctgga tccacgtttc
     9421 ttctctgttc ctgcaagcaa tgcgtgtggc tattccggcc gtcatcgttg cgctgtctgt
     9481 tggtaccagc gaagtacaga acatgctgaa tgcgattccg gaagtggtga ccaatggtct
     9541 gaatatcgcc ggtggcatga tcgtggtggt tggttatgcg atggttatca acatgatgcg
     9601 tgctggctac ctgatgccgt tcttctacct cggcttcgta accgcagcat tcaccaactt
     9661 taacctggtt gctctgggtg tgattggtac tgttatggca gtgctctaca tccaacttag
     9721 cccgaaatac aaccgcgtag ccggtgcgcc tgctcaggca gctggtaaca acgatctcga
     9781 taacgaactg gactaacagg tgagcgaaat ggttgataca actcaaacta ccaccgagaa
     9841 aaaactcact caaagtgata ttcgtggcgt cttcctgcgt tctaacctct tccagggttc
     9901 atggaacttc gaacgtatgc aggcactggg tttctgcttc tctatggtac cggcaattcg
     9961 tcgcctctac cctgagaaca acgaagctcg taaacaagct attcgccgtc acctggagtt
    10021 ctttaacacc cagccgttcg tggctgcgcc gattctcggc gtaaccctgg cgctggaaga
    10081 acagcgtgct aatggcgcag agatcgacga cggtgctatc aacggtatca aagtcggttt
    10141 gatggggccg ctggctggtg taggcgaccc gatcttctgg ggaaccgtac gtccggtatt
    10201 tgcagcactg ggtgccggta tcgcgatgag cggcagcctg ttaggtccgc tgctgttctt
    10261 catcctgttt aacctggtgc gtctggcaac ccgttactac ggcgtagcgt atggttactc
    10321 caaaggtatc gatatcgtta aagatatggg tggtggcttc ctgcaaaaac tgacggaagg
    10381 ggcgtctatc ctcggcctgt ttgtcatggg ggcattggtt aacaagtgga cacatgtcaa
    10441 catcccgctg gttgtctctc gcattactga ccagacgggc aaagaacacg ttactactgt
    10501 ccagactatt ctggaccagt taatgccagg cctggtacca ctgctgctga cctttgcttg
    10561 tatgtggcta ctgcgcaaaa aagttaaccc gctgtggatc atcgttggct tcttcgtcat
    10621 cggtatcgct ggttacgctt gcggcctgct gggactgtaa gactgttgta cactaccggg
    10681 gccttttggc cccgtttttt tatctggagg attaatgaca atcacggacc tggtactgat
    10741 tcttttcatc gccgcactcc tggccttcgc gatctacgat cagttcatca tgccccgccg
    10801 taacggcccc accctgctgg caattccttt gctccggcgt ggtcgcatcg atagcgttat
    10861 cttcgtcgga ttgattgtca ttcttatcta taacaacgtc acgaatcatg gtgcgttaat
    10921 aacgacatgg ttattaagcg cactggctct gatgggtttt tatatattct ggatccgcgt
    10981 tccgaagatc atctttaaac aaaaaggttt tttcttcgcc aatgtctgga ttgaatatag
    11041 ccgaatcaaa gcgatgaact tgtcggaaga tggcgtgttg gtgatgcaat tagaacagcg
    11101 tcggctgtta atccgcgttc gaaatatcga cgatctggaa aaaatttata agcttctcgt
    11161 ttcaactcaa taagttatga atttagccaa agctatgttt agtgtatttt taataatcag
    11221 acatagctta ggctatatta cctcttccct tatttgttat ttattttaac gtttcattga
    11281 tatataaatc caaatgaaaa tcgttatcaa taaagcaatg aaataatata ttccaacagt
    11341 tgttttatat tctcaaaata tgttaaggtt gcgccctcat ttggggagta gccgatttcc
    11401 agattccgga aatgtacgtg tcaacatact cgttgcaaaa cgtggcacgt acggactgaa
    11461 tactttcagt caggcgagac catatgcaca tcaatcgcta tgcctgcatg aacgcgaaaa
    11521 ccatcacggc ttgttgttca tgtatgcatg tttgctgggg gcgatgatgt gttttatgga
    11581 taccccggtc aggacattgt catgaatatc actgctactg ttcttcttgc gtttggtatg
    11641 tcgatggatg catttgctgc atcaatcggt aaaggtgcca ccctccataa accgaaattt
    11701 tctgaagcat tgcgaaccgg ccttattttt ggtgccgtcg aaaccctgac gccgctgatc
    11761 ggctggggaa tgggcatgtt agccagccgg tttgtccttg aatggaacca ctggattgcg
    11821 tttgtgctgc tgatattcct cggcgggcga atgattattg agggttttcg tggcgcagat
    11881 gatgaagatg aagagccgcg ccgtcgacac ggtttctggc tactggtaac caccgcgatt
    11941 gccaccagcc tggatgccat ggctgtgggt gttggtcttg ctttcctgca ggtcaacatt
    12001 atcgcgaccg cattggccat tggttgtgca accttgatta tgtcaacatt agggatgatg
    12061 gttggtcgct ttatcggctc aattattggg aaaaaagcag aaattctcgg cgggct
//
LOCUS       DAZPAC010000120        12037 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZPAC010000120 DAZPAC010000000
VERSION     DAZPAC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.120"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..323
                     /locus_tag="ACHUPM_002759"
     CDS             <1..323
                     /locus_tag="ACHUPM_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870489.1"
                     /translation="ASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIA
                     IFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPA
                     SNVD"
     gene            457..1534
                     /gene="ygjJ"
                     /locus_tag="ACHUPM_002760"
                     /pseudo
     CDS             457..1534
                     /gene="ygjJ"
                     /locus_tag="ACHUPM_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1551..3902
                     /gene="ygjK"
                     /locus_tag="ACHUPM_002761"
     CDS             1551..3902
                     /gene="ygjK"
                     /locus_tag="ACHUPM_002761"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR0870490.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            4128..6146
                     /gene="fadH"
                     /locus_tag="ACHUPM_002762"
     CDS             4128..6146
                     /gene="fadH"
                     /locus_tag="ACHUPM_002762"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR0870491.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6191..6607)
                     /gene="higA"
                     /locus_tag="ACHUPM_002763"
     CDS             complement(6191..6607)
                     /gene="higA"
                     /locus_tag="ACHUPM_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR0870492.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6604..6918)
                     /gene="higB"
                     /locus_tag="ACHUPM_002764"
     CDS             complement(6604..6918)
                     /gene="higB"
                     /locus_tag="ACHUPM_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR0870493.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="ACHUPM_002765"
     CDS             complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="ACHUPM_002765"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR0870494.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8424..8927
                     /locus_tag="ACHUPM_002766"
     CDS             8424..8927
                     /locus_tag="ACHUPM_002766"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR0870495.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9004..9687
                     /locus_tag="ACHUPM_002767"
     CDS             9004..9687
                     /locus_tag="ACHUPM_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR0870496.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9766..10752
                     /locus_tag="ACHUPM_002768"
     CDS             9766..10752
                     /locus_tag="ACHUPM_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0870497.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            11035..12000
                     /gene="alx"
                     /locus_tag="ACHUPM_002769"
     CDS             11035..12000
                     /gene="alx"
                     /locus_tag="ACHUPM_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR0870498.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2862 a         3094 c         3225 g         2856 t
ORIGIN      
        1 cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta cgcgccaaat
       61 tagatcacct gccacgcgat ttccgtatgg gctcccgccg caccggtatt atcgttgttt
      121 caatgctgat tgcgatattt gccgtagggt ttgtcgcttc gacattcccg actggcgcga
      181 atattctgac catcattttt tataacgtcg gcggtattgt tatcttcctt ggctttgcgt
      241 ggtggaaata cagtaaatat ataaagggat taacggctga agagcgccat attgaagcga
      301 cgccagccag caatgttgat taagcagaat aataaaaaat gagcaggcat tttccctctt
      361 cctggatgag gagagggaaa atagtttctg cctttatatt tttataatta caacgataaa
      421 aggctgtact ttttctttag ctcatggatt aacacaatga aattaatcac tgcaccatgc
      481 agagcattac ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt
      541 ccggcagaac atgacgacac aaaaacaccc gcaattacct cgacatcttc tccttcattt
      601 cgtttttacg gcgaattagg gattaggggg tggtggatat atggatttag agggtgagaa
      661 taaacataaa tacagcgacg gtacctatat tgaaggtggc ctggagatga agtacggctc
      721 ctggttcggc ctgatttacg gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc
      781 ctgggtgcca gaccatagct ggggtggttt cgagggcgga attaaccgtt tctatggcgg
      841 ttatcgtacc aatgatggca ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga
      901 tgacctgcaa tggtggggcg atttcacccc cgatctgggc tacgtcattc ccaatacccg
      961 cgacattatg actgcgctga aggtacagaa cttaagcggc aactttcgtt atagcgtcac
     1021 cgcgactcct gccggacatc atgatgaaag caaagcctgg ctacattttg gcaaatacga
     1081 tcgctatgac gacaaataca cctatccggc aatgatgaac ggttacatcc agtatgacct
     1141 tgccgaaggc atcacctgga tgaacggtct ggaaatcacc gacggcacag gacagctcta
     1201 tctcacgggc ctgctaactc ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc
     1261 cgacgggctg gacgtaccgg gaagtgaaag tgggatgatg gtgagcgcca tgtatgaagc
     1321 gttaaagggc gtttatctct ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc
     1381 tgacgatgaa accacctctt tcatgcagtt tggtatctgg tacgaatacg gcggcggacg
     1441 tttcgccacg gcttttgata gccgcttcta catgaaaaat gcctctcacg atcccagcga
     1501 ccaaatcttc ctgatgcaat atttctactg gtaataagga ctgtaatttc atgaaaataa
     1561 aaactatttt aacgccagta acctgcgctc tgctgataag tttttccgcc catgccgcta
     1621 acgccgacaa ttataaaaac gtgattaacc gtactggcgc gccgcagtac atgaaggatt
     1681 acgattacga cgatcaccag cgttttaatc cgtttttcga tctcggagcc tggcatggtc
     1741 atctgttgcc agacggccct aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg
     1801 aagagtacat caactttatg gccagcaatt tcgaccgcct gaccgtctgg caggacggca
     1861 agaaagtcga ctttacgctg gaggcataca gtattcccgg tgcgctggtg caaaaactga
     1921 cagcaaaaga tgtgcaagtc gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac
     1981 tggaaaccaa aatcaccagc aataaaccgc tggatctggt gtgggacggc gaactgctgg
     2041 aaaaactgga agcgaaagaa gggaaaccgc tttccgataa aaccattgct ggcgaatacc
     2101 ctgactatca gcgcaaaatc agcgccaccc gtgatggcct gaaagtcacc tttggcaaag
     2161 tgcgcgccac ctgggatctg ctgacctccg gcgaatcgga atatcaggtg cataaatccc
     2221 tgccggtgca gactgaaatc aacggtaatc gctttaccag taaggcgcat atcaacggtt
     2281 cgaccacgct ctacaccacc tattcccatc tgctgaccgc tcaggaagtt agcaaagagc
     2341 aaatgcagat ccgcgatatt ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac
     2401 gctgggaaga atacctgaag aaagggttaa ccaatccgga tgcgacgccg gaacagacgc
     2461 gcgtcgcggt gaaagcgatc gaaacgctca acggtaactg gctctcaccg ggcggtgcgg
     2521 tgaaatttaa caccgttaca ccgtcggtga ccgggcgctg gttctccggc aatcagacct
     2581 ggccgtggga tacctggaag caggcgtttg cgatggcgca tttcaatccg gacatcgcca
     2641 aagagaatat ccgcgcggtc ttctcctggc agatccagcc tggcgatagc gtgcgtccgc
     2701 aggatgtggg ctttgtcccc gacctgatag cgtggaacct tagccccgag cgtggtggcg
     2761 atggcggcaa ctggaacgaa cgtaatacca aacccagcct tgccgcctgg tcggtgatgg
     2821 aagtgtacaa cgtcacccag gataaagcct ggttggcaga gatgtacccg aaactggtgg
     2881 cctatcacga ctggtggtta cgtaaccgcg atcacaacgg caacggcgtg ccggaatatg
     2941 gcgcgacccg cgacaaagcc cacaacactg agagcggcga gatgctgttt acggtgaaaa
     3001 aaggcgataa agaagagacg cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag
     3061 gcctgtacga cagtctggaa attccggcac aggttgctgc atcgtgggaa tcggggcgtg
     3121 atgatgccgc cgtctttggg tttatcgaca aagaacagct ggataaatat gtcgctaatg
     3181 gcggcaaacg tagcgactgg acggtgaaat tcgccgaaaa ccgcagtcag gacggaacgt
     3241 tgctgggcta ctcgctattg caggagtcgg tggatcaggc cagctatatg tacagcgata
     3301 accattatct ggcggagatg gcaaccatcc tcggtaagcc agaagaggcc aaacgctatc
     3361 gccagttggc acagcagctc gcggactaca tcaacacctg tatgttcgac ccgactaccc
     3421 agttctacta tgacgtacgt attgaagata aaccactggc gaacggctgc gcgggcaaac
     3481 cgattgttga gcgcggtaaa gggccggaag gctggttgcc gctgtttaac ggtgcggcaa
     3541 cgcaggccaa cgccgacgcg gtggtgaagg tgatgctcga tcctaaagag ttcaacacct
     3601 ttgtcccgct gggaacggcg gcgttaacca atccggcttt tggcgctgat atctactggc
     3661 gtgggcgcgt atgggtggat cagttctggt ttggtctgaa agggatggag cgttacggtt
     3721 atcgtgatga tgccctgaag ctggcggata cgttcttccg gcacgccaaa ggattaaccg
     3781 ccgatggccc aattcaggag aattacaacc cgctgactgg cgcacagcaa ggcgcaccaa
     3841 atttctcctg gagtgccgct catttgtata tgttgtataa cgattttttc cgtaagcagt
     3901 aatttgcaac ggctggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc
     3961 ccaggccgga taaaacgcgg caagcgttgc atccggcgtt accaccgggc gtattctttt
     4021 tgaatcccat cacaaacccc gcactcccct tttccctttt ctccatcgac ggctaaatta
     4081 gaactcatcc gaccacataa caattatttt acatactgga caattttatg agctatccgt
     4141 cgctgttcgc cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct
     4201 caatgcacac cgggctggag gaatacccgg acggtgccga gcggctggca gcgttttatg
     4261 ccgaacgcgc ccgtcacggc gtggcgctga ttgtcagcgg cggcattgca ccagatttaa
     4321 caggcgttgg catggaaggt ggcgcaatgc tcaacgacgc cagccagatc ccacaccatc
     4381 gcaccattac cgaagcggta catcaggaag gcggcaaaat agccctgcaa attttgcata
     4441 ccgggcgcta cagctaccaa ccgcatctgg tcgctccctc cgcattgcag gcccccatca
     4501 accgtttcgt tcctcacgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg
     4561 cccactgcgc gcaactggca cgggaggcag gatacgacgg tgtagaggta atggggtccg
     4621 aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa tcagcgtagt gaccagtggg
     4681 gcggcgatta ccgcaaccgg atgcgatttg ccgtagaagt agtgcgtgcg gtgcgcgaac
     4741 gcgtcggcaa cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaagacg
     4801 gcgggacttt tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca
     4861 ttatcaacac cggcattggc tggcatgaag cacgtattcc gaccattgcc acgcccgtgc
     4921 cgcgcggcgc atttagctgg gtcacgcgca aattgaaagg ccacgtctcg ctgccgctgg
     4981 taaccaccaa ccggattaac gatccgcagg ttgccgacga tattctctcg cgcggcgatg
     5041 ccgatatggt atcgatggca cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc
     5101 aatcggggcg agccgatgag atcaacacct gcattggctg caatcaggcc tgcctcgatc
     5161 agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgc cacgaaacca
     5221 aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg
     5281 ctgggctggc gtttgccatt aacgcggcgg cgcgtgggca tcaggtaaca ttgtttgatg
     5341 cccacagcga gatcggcggg cagtttaata tcgccaaaca gatccccggc aaagaggagt
     5401 tttacgaaac gctgcgctat taccaccgga tgatcgaagt gacgggcgtg acgctgaaac
     5461 tcaatcacac cgtgacggcg gatcagttac aggcgttcga tgaaacgatc ctcgccagtg
     5521 ggatcgtgcc gcgcgttccg cctatcgacg ggatcgatca tccgaaggta ttgagttatc
     5581 tcgatgtact gcgcgacaaa gcgccggtag gcaacaaggt tgccatcatc ggttgtggcg
     5641 ggattggttt tgatacggcg atgtatttaa gtcagccggg cgaatccacc agccagaata
     5701 tcgccaggtt ctgtaatgag tgggggatcg acagcagcct acaacaggct ggtggcttaa
     5761 gcccgcaggg aatgcagatc ccgcgtagcc cacggcaaat tgtgatgctc cagcgcaaag
     5821 ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc
     5881 tctcgcgggg tgtgaaaatg atcccaggcg taagttatca gaagattgac gatgacgggc
     5941 tgcatgtggt gatcaacggc gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg
     6001 cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc
     6061 atttaattgg cggctgcgat gtggctatgg agctggacgc acgacgggca attgcccagg
     6121 gaacacggct ggcgctggag atttaaatcg ttattttaag cgccggatgc aacgcatccg
     6181 gcacgtcgcg ttaatcaata aacaaggcgg gagaaatgcc gaatcgcgtt gccaattttt
     6241 tagcgtgttc cagcgttaat ttcctcttcc cgctcaaaac gcgtgacacc atagatttac
     6301 tgccaatttc cggcagatcg gcaagggtta aaccatattg atccataagg gtacgaatca
     6361 cggctatacc gccaggcatg gcttgagcca tggcattaaa ttccgcaaat tcgggcgctg
     6421 attcttccca cgcggttatt ttggcacaca ccagatccag caaggggttt tcaggatcgt
     6481 tgagcagcag atgatctacc agttccagcg cctgggtgta ttgttcctcg ttctgaatac
     6541 ccgccagaaa aggtgccacc gcagttagct tttctcctgc ttgcaagatg tcggcaatcg
     6601 caatcatttt ttccccttag tacgatgaac agcggtaaag aaatcgtatt ctttatgcgt
     6661 cataacttca cgtatgtagc acttttgcga ttcaaaaaag accattgcta caacacgtaa
     6721 ttcattgccc ccaacattga aaacataatg cttatccaga tatttgaagt tatccagaga
     6781 tgggaatact gcttttaatg actcaggttt tttgaaatat cccttagcaa tcgtgttccc
     6841 cagagccacc aactccgttt tatgttgcgg gtatttttcc gcagcatctt tcaatgcttt
     6901 ttgagttatc aggtgcattc ttcatcacgt ccgttgacaa attggcaata tgataacatc
     6961 cgttgacaga ttggcaacgg atgaatttat ctgtgtgacg gaagaataaa cgtagaaaaa
     7021 cgctaaacaa gagaatgtag aggatggtga gagggatttt ttgttttatg gaagcgcgat
     7081 tccagaactg ggcagacatt gccggatgcg acgcttgccg cgtcttatcc ggcctgggag
     7141 taacgtgctg aacgtaggcc ggataaggcg ttaacgccgc atccggcagg taactcccaa
     7201 tcttaccgac gacgccccag cttcactgct ttcagcacca caaatttatt attcgtggcg
     7261 atggtggtgc agttgccgaa aatcttcttc agtttatgga agtaatccag gtgacggttg
     7321 gcaacaatat acagctcgcc gttgattttc aggcagcggc gggcatggtg gaacatctcc
     7381 caggcgacgt tatcggtcag cgcatgttgt tggtgaaacg gcgggttgca gagcacagca
     7441 ttaaagcgga aaggctccac gccggagagc gcgttgttga tcataaactc gctgcgatct
     7501 aacgcctctg gcatgttggt ttcaacgttc aaacggctgg aagcaaccgc catcggcgat
     7561 tcatcgacaa acaccacttt cgcctgaggg tttttatcaa gcagcgtcag accaataacg
     7621 ccattaccgc aaccgagatc gacaatctca ccttcgagat tctctggcag atgttgcata
     7681 aagaagcgtg cgccaatatc cagcccggtg cgggagaaga cattcgcatg gttgtggata
     7741 gtccagtcag taccttccag cttccagcta acggtctgcg gcgcatcggc cagcggcggt
     7801 tcattgaaag tgcaattaat caggcgcgct tttttccatg ccagcgtagt ggtggtcggg
     7861 ccgagcactt tctcgaacag ctccagcgtg gaagtgtgaa tgtcacgggc tttggcaccg
     7921 gcaataatac gtgtatccga cgtgaccact ttgcgcagcg cacgcagttg ctgttccagc
     7981 aatgccagtg ttttcggcac tttgatcagc accacacccg gctgttgcgg gtagtcggcg
     8041 gtgctgtcga gaaacttcac gctcgactca tcaatcccgt tgaggcgtaa attctcgcgc
     8101 gtcgccagtt cactgatgta tgagtcgcca atgctgtacg gcttatgttc cgccagcgcg
     8161 cagcttaacg caccaaaggc atcattcagg atcaacaccg ggccgcggat ttctgtgtcg
     8221 tccaactgtt gcagcaaata ttcatccgcc gcttcccacg cctgtagcgg gttaacgtca
     8281 tccgtcgccg gaaaacgttg tagtgtcagt gaacggaaac cgttgtctaa gtggctcatc
     8341 ggccctcctg aatgataaaa tttcgacgtt gcccctgaaa agggtgcgtg agtataccct
     8401 ttttctcttt ttcgtgggcg gttatgagca atcttactta tcttcagggt tatcccgagc
     8461 agctactttc ccaggtgcga acgctgatta acgaacagcg tctgggcgat gtgctggcaa
     8521 aacgctatcc gggaacacac gactacgcca ccgataaagc cctctggcaa tatacccagg
     8581 atctaaaaaa tcagtttctg cgcaatgccc cgccgatcac taaagtgatg tatgacaata
     8641 agatccacgt actgaaaaac gcgctcggat tacataccgc tgtttctcgt gtgcagggcg
     8701 gcaagctgaa agcaaaagca gagatccgcg tcgctactgt gtttcgcaac gcgccggaac
     8761 cgtttttacg catgatcgtg gtgcacgaac tggcgcacct gaaggagaaa gagcataaca
     8821 aagcgtttta ccagttgtgc tgccatatgg agccgcagta ccaccagctt gagttcgaca
     8881 cccggctgtg gctaacgcag ttgtcgcttg ggcaggacaa aatctgaaaa tgatgtcagg
     8941 cgttatcttt tctttcttta gcgcctaaaa tcgacctccc ccctttcgtt aaggataacg
     9001 accatgcagc cccgccttct tctccgtatt tgtttttctc gccggactct aaagattacc
     9061 tgcttactgt tacttgtggc aggagcgacc atttttatcg ccgatcgcgt aatggtcaac
     9121 gccagcaagc agctgacctg gagcgatgtc aacgcagttc cggcgcgtaa cgtgggttta
     9181 ttgctggggg cgaggccggg taatcgctac tttacccgac gtattgatac cgcagcagcg
     9241 ttgtaccacg tcggaaaagt gaaatggctg ttagtcagtg gcgataacgg acgtaaaaat
     9301 tacgatgaag cctcaggtat gcagcaggct ttgatcgcta aaggcgtacc ggcgaaagtg
     9361 atcttctgcg actatgccgg attctcaacg cttgattcag tggtgcgtgc caaaaaggtg
     9421 tttggggaaa accatatcac cattatctcg caagaatttc ataaccaacg cgccatctgg
     9481 ctggcaaaac agtacggcat cgacgctatc gggtttaacg cgccagacct taatatgaag
     9541 cacggttttt atactcaact gcgggagaaa ctcgctcgtg tcagcgcggt gattgacgcc
     9601 aaaatcctcc atcgccagcc gaaatatctc gggccatcgg tcatgattgg gccgtttagt
     9661 gagcatggct gtccggcgaa agaataatgc gtatctgcgc acgtcgaaga tgaaaaaggc
     9721 gtgctacatt gacgacagaa tccctttatg gagtatccac gcgttatgat acgtttcgct
     9781 gtgattggta cgaactggat cactcgccag ttcgtcgagg ccgcccatga gagcggtaaa
     9841 tacaagttaa ccgccgtata ttcccgcagc cttgaacagg cccagcactt cgccaatgat
     9901 ttttctgtcg agcatctgtt tacctcgctg gaagcgatgg cggaaagcga tgccattgac
     9961 gcggtgtata ttgccagccc gaatgccctg cacttttccc agacacaact tttccttagc
    10021 cataaaattc atgtgatttg cgagaaacca ctggcgtcga atctggcgga agtggatgcc
    10081 gccattgcct gtgcgcggga aaatcaggtg gtgctgtttg aggcatttaa aaccgccagc
    10141 ctgccgaact ttcatttgct gcgccaggcg ctgccgaaag tcggcaaatt gcgtaaagtc
    10201 tttttcaact attgccagta ttcctcgcgg tatcaacgtt acctggatgg tgagaatccc
    10261 aacaccttta atccggcatt ctctaacggt tcaattatgg atatcggctt ttactgcctg
    10321 gcgtcggcgg tggcattatt cggtgaaccg aaaagcgtgc aggcaaccgc cagtttgttg
    10381 gcaagcggcg tagatgccca cggcgtggtg gtgatggatt acggtgattt cagcgttacg
    10441 ttgcagcact caaaggtcag tgattctgtc ctggaaagcg agattcaggg cgaagcggga
    10501 tcgctggtga ttgaaaaact gtctgaatgc cagaaagtgt gcttcgtgcc gcgtggcagc
    10561 cagatgcagg atctcaccca gccgcagcat attaatacca tgctctacga agcagagctg
    10621 tttgctaccc tggtggatga gcatctggtg gatcatccag ggctggcggt cagtcgcatc
    10681 accgccaaac tgctgactga gatccgccgc cagactgggg tgatttttcc ggcagatagc
    10741 gtaaaactat aattgccaaa gtaaaacagt gtaaaaggta tgtaacagac cattgactgg
    10801 ctgaatggtc tgtcatactt tgttacctgc aaaggggagt aacttcattg ccggtcgatc
    10861 gtcattacga tgtgtgaaaa accacatccg gtcaccgggc aacccgaaag gaatacgcag
    10921 acgtattcct tttttgttgt aagtgagacc ttgccggaag gcgaggtcta tgcataaaaa
    10981 gcagcggctg acgtcttccg acgttggccg tttttttatg tgtaaggaac ttctatgaat
    11041 actgtcggca cgccgttgct atggggcgga ttcgctgttg ttgtcgccat tatgctggct
    11101 atcgacctgt tgttgcaggg gcgtcgtggg gcacatgcca tgaccatgaa acaggctgcg
    11161 gcctggtcgc tggtctgggt gacgctgtcg ttactgttta acgccgcttt ctggtggtat
    11221 ctggtgcaaa ctgaaggtcg cgccgttgcc gatccacagg cactggcctt tctcacgggt
    11281 tatctgattg agaaatcgct ggcggtcgat aacgtctttg tctggctgat gttgttcagc
    11341 tatttctctg ttccggcggc attacaacgc cgcgtgctgg tgtatggcgt gctcggggcg
    11401 attgttctgc gtaccatcat gatcttcact ggcagctggc taatttcgca gttcgactgg
    11461 atcctgtata tcttcggtgc cttcctgctg tttaccggcg tgaagatggc gctcgcccat
    11521 gaagatgaat caggcattgg cgacaagccg ctggtgcgct ggctacgcgg tcatttgcgc
    11581 atgaccgaca ccatcgacaa cgagcatttc tttgtgcgta agaatggatt gctgtacgcc
    11641 acaccgctga tgctggtact gattctggtg gaattgagcg acgtgatttt cgccgtggat
    11701 agcattccgg ctatcttcgc cgtgaccact gacccgttca ttgtgctgac ctcaaacctg
    11761 tttgcgatcc tcggcctgcg tgcgatgtat ttcctgctgg cgggcgtagc agagcgtttc
    11821 tcgatgctca aatatggcct ggcggtgatt ctggtgttta tcggtatcaa gatgctgatt
    11881 gtcgacttct accatattcc aatcgccgtc tcgctgggcg tggtgtttgg cattctggtg
    11941 atgacgttta ttatcaacgc ctgggtgaat tatcggcatg ataagcagcg gggagaatga
    12001 ttcgctgtat attaagccat tcctagagat aactcat
//
LOCUS       DAZPAC010000121        11960 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZPAC010000121 DAZPAC010000000
VERSION     DAZPAC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.121"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1760
                     /locus_tag="ACHUPM_002770"
     CDS             <1..1760
                     /locus_tag="ACHUPM_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR0870499.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
     gene            2096..3073
                     /gene="glaH"
                     /locus_tag="ACHUPM_002771"
     CDS             2096..3073
                     /gene="glaH"
                     /locus_tag="ACHUPM_002771"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR0870500.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3093..4361
                     /gene="lhgO"
                     /locus_tag="ACHUPM_002772"
     CDS             3093..4361
                     /gene="lhgO"
                     /locus_tag="ACHUPM_002772"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR0870501.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4384..5832
                     /gene="gabD"
                     /locus_tag="ACHUPM_002773"
     CDS             4384..5832
                     /gene="gabD"
                     /locus_tag="ACHUPM_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0870502.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5846..7126
                     /gene="gabT"
                     /locus_tag="ACHUPM_002774"
     CDS             5846..7126
                     /gene="gabT"
                     /locus_tag="ACHUPM_002774"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFR0870503.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7437..8837
                     /gene="gabP"
                     /locus_tag="ACHUPM_002775"
     CDS             7437..8837
                     /gene="gabP"
                     /locus_tag="ACHUPM_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR0870504.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8858..9520
                     /gene="csiR"
                     /locus_tag="ACHUPM_002776"
     CDS             8858..9520
                     /gene="csiR"
                     /locus_tag="ACHUPM_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR0870505.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="ACHUPM_002777"
     CDS             complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="ACHUPM_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR0870506.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10054..10212)
                     /locus_tag="ACHUPM_002778"
     CDS             complement(10054..10212)
                     /locus_tag="ACHUPM_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR0870507.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10395..10694
                     /locus_tag="ACHUPM_002779"
     CDS             10395..10694
                     /locus_tag="ACHUPM_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR0870508.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10704..11228
                     /gene="ygaP"
                     /locus_tag="ACHUPM_002780"
     CDS             10704..11228
                     /gene="ygaP"
                     /locus_tag="ACHUPM_002780"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR0870509.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11275..11679)
                     /gene="stpA"
                     /locus_tag="ACHUPM_002781"
     CDS             complement(11275..11679)
                     /gene="stpA"
                     /locus_tag="ACHUPM_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR0870510.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2985 a         2947 c         3078 g         2950 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctagcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa
     1681 ctctaaatat caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc
     1741 cgaactatct ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat
     1801 atgtttagtt tactaacggt cattttgcag tgaagccatt tactgttttt tatcgaccag
     1861 ataatctgtt ctctaatgtt aactccccct aacctgttgc tttagttatt catttcctgt
     1921 ctcactttgc cttaatactc tacgttaaat gttactaatt tgttgctttt gatcacaata
     1981 agaaaacaat atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
     2041 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
     2101 tgcactgacc gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac
     2161 tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac
     2221 caaacagttt ctcgagcagg ttgccgaatg gcccgtacag gcgctggagt acaaatcgtt
     2281 tctgcgtttt cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt
     2341 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtat
     2401 cgatgatgtg gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat
     2461 tggtcgctca aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa
     2521 tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga
     2581 cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa
     2641 catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta
     2701 tttccgccat ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt
     2761 cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg
     2821 ctatatcgac cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct
     2881 ttccgatgcc attgaaacca gcaaaggcat tctttctgta cccgttcccg ttggcaaatt
     2941 cctgttgata aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga
     3001 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactacca
     3061 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     3121 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ttatccggat gcccgcattg
     3181 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     3241 tccatgccgg ggtctattac acgcccggca gcctgaaggc gcagttttgc ctggcgggga
     3301 accgcgccac caaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga
     3361 tgctggtcgc cacgtctcag ctcgaaatgg aacggatgcg cgccttatgg gaacgcacag
     3421 cggcgaacgg tatcgagcgc gagtggataa acgccgtgga actgcgcgag cgcgaaccga
     3481 atatcaccgg gcttggtggc atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     3541 tcacagcggc aatggcaaaa atcttccagg ccagaggcgg cgagattatt tataacgccg
     3601 aagtcagcgc cctcagtaag cataaaaacg gcgtggtgat acgcacccgt cagggcggtg
     3661 aatatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     3721 tgctcggcct cgaaccgggc tttatcatct gcccgttccg tggtgagtat ttccgccttg
     3781 cgccggagca taaccagatt gttaaccacc tgatttaccc cattcccgac cccgcgatgc
     3841 catttttggg cgtgcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     3901 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agtgacacgc
     3961 tggagatttt gagctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     4021 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctacct gcggctggtg caaaagtatt
     4081 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcag
     4141 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccacccca cgcacgatcc
     4201 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     4261 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     4321 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta
     4381 acaatgaaac ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     4441 ctggacgcca acaatggcgg gatcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     4501 ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc
     4561 gccctgcccg cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg
     4621 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggc
     4681 aaaccactgg ccgaagcgaa aggcgaaatc agctacgctg cctcttttat tgagtggttt
     4741 gccgaagaag gcaaacgtat ttatggcgat accattcccg gtcatcaggc cgataagcgc
     4801 ctgattgtta tcaagcagcc gattggcgtt accgcagcta tcacgccgtg gaacttcccg
     4861 gcggcgatga ttacccgtaa agccggtccg gcgctggcgg caggctgcac catggtgctg
     4921 aagcccgcca gtcagacacc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg
     4981 ggcattccgg ctggcgtgtt taacgtagtc accggttcgg caggcgcggt cggtaatgaa
     5041 ctgaccagca acccgctggt acgcaaactg tcgtttaccg gttcgaccga aattggccgc
     5101 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     5161 gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcgctggcc
     5221 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     5281 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc
     5341 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     5401 gtggaagagc atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa
     5461 gcggatgaac gcggcggcaa cttcttccag ccgaccattc tggtggacgt gccggccaac
     5521 gccaaagtgt cgaaagaaga gacgttcggc cccctcgccc cgctgttccg ctttaaagat
     5581 gaagccgatg tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac
     5641 gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc
     5701 atcaataccg gcattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt
     5761 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     5821 atcggtcttt aactggagaa tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag
     5881 tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa
     5941 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt
     6001 gctcaatacc gggcacctgc atccgaaggt ggtggccgca gtggaagcgc agttgaaaaa
     6061 actgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat
     6121 tatgaatcag aaggtgccgg gcgatttcgc caagaaaacg ctgctggtta cgaccggttc
     6181 cgaagcggtg gaaaacgcgg taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat
     6241 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     6301 gaatccgtac tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc
     6361 ttgcccgctg cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa
     6421 aaatgatgcc gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     6481 cggtttctac gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca
     6541 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     6601 tgcgatggag cagatgggcg tggcgccgga tcttaccacc tttgcgaaat cgatcgcagg
     6661 cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg
     6721 cggtctgggc ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     6781 gaaggtgttc gagcatgaaa atctgctgca aaaagccaac gatctggggc agaagctaaa
     6841 agacggattg ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     6901 agcgatgatt gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact
     6961 caccgccgag atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc
     7021 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     7081 gggtctggag atcatcagcc agtgttttgc tgaggcgaag cagtagctat gtccgtttgt
     7141 aagagttaac cgttgctaag ggaatgataa cgatgcagta aaaaataata acgatgaggc
     7201 gaacgacaaa gttaaaaacg actgcggaca gtcccctcgc ccctctggag agagggttag
     7261 ggtgaggggt aaaacacgcg gctaaaccct cccctggccc tctccctaag ggagagggag
     7321 aaaaccctcc cgctttcgtt tttaagattt tgttgaccgt ctcaattaaa cgcgtgaccc
     7381 ggcgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg
     7441 ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa atcacgccac gtcaccatgt
     7501 tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg
     7561 aagcgggccc ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta
     7621 tgcggatgtt ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg
     7681 ccgataaagc cattggtcgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt
     7741 gggtactggt tatcccgctg gaagccaata tcgccgctat gatcctgcac tcgtgggttc
     7801 caggcattcc catctggtta ttttccctcg tcattaccct tgccttaact ggcagtaatt
     7861 tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg
     7921 ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg
     7981 aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg
     8041 cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca
     8101 ttgccgccgc ggaatccgac acgccggaaa aacatattgt ccgcgccacc aactcggtta
     8161 tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt
     8221 ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggttctggaa ttgctcaata
     8281 ttccccatgc gaaattaatc atggactgtg tgatattact ttccgtaacc agttgcctga
     8341 actcggccct gtataccgcc tcaaggatgc tctactcctt aagccgtcgc ggtgatgctc
     8401 ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta cgtggcggtg ttactctcca
     8461 ccggcgcggc attcttaacg gtggtggtga actattacgc gcctgcgaag gtatttaaat
     8521 ttctgatcga cagctccggc gctatcgccc tgctggttta tttagtcatc gccgtttcac
     8581 agttgcggat gcgcaaaatt ctgcgagcgg aaggaagcga aattcgcttg cgaatgtggc
     8641 tttatccctg gctcacctgg ctggtcatcg gctttattac ctttgtgttg gtagtgatgc
     8701 tattccgtcc ggcgcaacag ttagaagtga tctccaccgg cttattagcg atagggatta
     8761 tctgtaccgt gccaattatg gctcgctgga aaaagctggt attgtggcaa aaaacacccg
     8821 ttcataatac gcgctgataa tgatcaggag tcacaccatg accattacgt ctctggatgg
     8881 ctatcgctgg ctgaagaacg atattattcg cggtaatttt caaccggatg aaaaattacg
     8941 gatgagtttg ctgacatcgc gttatgcact tggcgttggg ccgttgcggg aagcactttc
     9001 tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc gggtggcgtc
     9061 tatgtcagag caggagctgc tcgatatttt cgatgcccgc gccaatatgg aagcgatgtt
     9121 agtgagtctg gcgattgccc gcggtggcga tgagtgggag gcagacgttc tcgcaaaagc
     9181 gcatctgctc agtaagctgg aagcctgtga cgccagcgag aaaatgcttg atgagtggga
     9241 tctgcgtcat caggcgtttc atacagcaat tgtggcgggc tgtggttctc actatttgct
     9301 gcaaatgcgt gaacggttgt ttgatctggc ggcgcgctat cgatttatct ggctgcggcg
     9361 aacggtgctt tcggtagaaa tgctggagga taaacacgat cagcaccaga ccctgactgc
     9421 ggcggtactg gcgcgagata ccgcgcgcgc cagtgagtta atgcgccagc atttactgac
     9481 gccaattccc attatccagc aggcgatggc tggcaattaa ctactcttcc ggaatacgca
     9541 acacttgccc cggataaatt ttatccgggc tttttagcat cggtttattc gcttcgaaga
     9601 ttttattgta cagattagcg ttaccgtaga cctgtttgga aatggcactc agagtgtcgc
     9661 cagacttaac ggtataaaac tggctggcag tggctggtgt cgccgttttc acctgatcat
     9721 caacactggc aataccggaa atattcccca ccgcaacaag gattttctcc ttcgcctcct
     9781 gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc aatttgaatg ttcactttat
     9841 cagcatcagg tataccggtt ttgctcagat gctcctgcac cttcttcgcc tgatcgtctt
     9901 tatcgtgctg acctgtaacc gcgtcccaga gtttttctcc ggcatctttc acaaaattga
     9961 acagacccat agctacctca ttcgttaacg gaaacactca gaaagtgtag cagaggaacg
    10021 cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg cgggtttgca cccagaacgc
    10081 gtgaatcaaa ccggggatat acccaagcag tgtcagcaga atattaataa tgaacgccca
    10141 accgaaccct ttaccgagca gcacgccgag cggcggtaga atgatggtga tgacgattct
    10201 ccagaaaccc atatgtactc cctataagaa aattactcat tgtttaaaaa gagattttat
    10261 ctcttaagcg tagtaatttt agcggagcct gccagtttca gcttacgctt ttactctcat
    10321 ttacgctgct gaggctggta tatgttgcat ttttagttta gagcttactt aatttagaaa
    10381 atacttaaat aaatatgact gaactcgcgc aattacaggc cagtgccgaa caggcagcgg
    10441 ccttattgaa agcaatgagc catcctaaac gattgctgat tctgtgcatg cttagcggtt
    10501 cccccggcac cagcgcggga gagctgacgc gcattaccgg actgagtgcc tctgcgacat
    10561 cacagcatct cgctcgtatg cgggacgaag ggcttatcga cagccaacgg gatgcccaac
    10621 gcattctata ttccattaaa aatgaggcgg taaatgccat tatcgccacc ctgaaaaatg
    10681 tctattgtcc gtaaggagtc actatggctt tgacaaccat ttcgccgcat gatgcacaag
    10741 aattaatcgc acgcggcgca aagttaatcg atattcgtga cgctgatgaa tatcttcgtg
    10801 aacatattcc tgaagcagat ctggctccat tatccgtgct ggaacagtca ggtcttccac
    10861 ctaaattacg tcgcgagcaa attatattcc actgccaggc aggtaaacgc accagtaata
    10921 acgccgataa attagcagcg attgccgccc ccgcagaaat atttttactc gaagatggga
    10981 ttgatggctg gaaaagagct ggattgccag tagcggtaaa taaatctcaa cccttgccgt
    11041 taatgcgcca ggtgcagatc gctgcgggtg gtttaatatt aatcggcgtt gtactgggtt
    11101 ataccgtaaa tagcggtttc ttcttattaa gtggctttgt tggtgccggg ttactgtttg
    11161 caggaatcag cggtttttgt ggaatggcaa ggttgttaga taagatgccg tggaaccaac
    11221 gagcttgaga agcgacgccg gacgcgccct agcagcgaca tccggcctca gtaattagat
    11281 caggaaatcg tcgagagatt taccttctgc cagcgcctga gcaattggct tcggtgtacg
    11341 gccctgaccg gtccaggttt tagtttcacc gttaacatcg gtgaatttat atttcgccgg
    11401 acgcggctgg cgttttttac cagcgcgtgg tgcagcagca gagctattac ccaataactc
    11461 ttccgggtta attccgtcag ccttcatcag ctccagccag gtgctaattt tttcctgacg
    11521 ctcagccagt tcacgctgct gctgttcttc ttcttcacgt ctttctttag tgacaaccct
    11581 gaatttttcg agcatttctt caagaacgtc aatggagaat tcgcgagcca tcgcacggag
    11641 ggtgcgaatg ttatttaaac tttgtaacat tacggacata ataaagaaaa ccttttaacg
    11701 ccaaaacaaa aaagtatttc gtcgcgtagt gtaagcgatc acttattata tttccacaaa
    11761 atttaatcta tttacagtct tgcgcgagat tatttcagct gttattttct gaaactctca
    11821 gaataatttc aagattatat attagccatt ttgatgagta atttagatta ttggtgaaca
    11881 gaacggcgct ctctgctcgc taattccgca agcctgacaa aaccgaagtg aataaaaatg
    11941 caatataaca gaataaaaga
//
LOCUS       DAZPAC010000122        11877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZPAC010000122 DAZPAC010000000
VERSION     DAZPAC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.122"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            486..1157
                     /locus_tag="ACHUPM_002782"
     CDS             486..1157
                     /locus_tag="ACHUPM_002782"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFR0870511.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1167..2363
                     /locus_tag="ACHUPM_002783"
                     /pseudo
     CDS             1167..2363
                     /locus_tag="ACHUPM_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2363..2923
                     /locus_tag="ACHUPM_002784"
     CDS             2363..2923
                     /locus_tag="ACHUPM_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR0870512.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2925..3698
                     /locus_tag="ACHUPM_002785"
     CDS             2925..3698
                     /locus_tag="ACHUPM_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFR0870513.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3886..4161
                     /gene="frmR"
                     /locus_tag="ACHUPM_002786"
     CDS             3886..4161
                     /gene="frmR"
                     /locus_tag="ACHUPM_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFR0870514.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4196..5305
                     /gene="frmA"
                     /locus_tag="ACHUPM_002787"
     CDS             4196..5305
                     /gene="frmA"
                     /locus_tag="ACHUPM_002787"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFR0870515.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5398..6231
                     /gene="fghA"
                     /locus_tag="ACHUPM_002788"
     CDS             5398..6231
                     /gene="fghA"
                     /locus_tag="ACHUPM_002788"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR0870516.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6457..6996)
                     /locus_tag="ACHUPM_002789"
     CDS             complement(6457..6996)
                     /locus_tag="ACHUPM_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFR0870517.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="ACHUPM_002790"
     CDS             complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="ACHUPM_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFR0870518.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8596..9609)
                     /gene="mhpE"
                     /locus_tag="ACHUPM_002791"
     CDS             complement(8596..9609)
                     /gene="mhpE"
                     /locus_tag="ACHUPM_002791"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFR0870519.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="ACHUPM_002792"
     CDS             complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="ACHUPM_002792"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFR0870520.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10553..11362)
                     /gene="mhpD"
                     /locus_tag="ACHUPM_002793"
     CDS             complement(10553..11362)
                     /gene="mhpD"
                     /locus_tag="ACHUPM_002793"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFR0870521.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11372..>11877)
                     /gene="mhpC"
                     /locus_tag="ACHUPM_002794"
     CDS             complement(11372..>11877)
                     /gene="mhpC"
                     /locus_tag="ACHUPM_002794"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR0870522.1"
                     /translation="AFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQP
                     TIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPR
                     LAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLV
                     LNFLARP"
BASE COUNT         2912 a         3177 c         2908 g         2880 t
ORIGIN      
        1 aatatgcaat tactccgttc taacaattgg gaaaattact acacttcatt ttagtgatgc
       61 ataaacaacc acattacgag atttattact tcattgtttc tatcaataca tataataaaa
      121 ctattatatt cgcattgtat atatcactgt atcattctca atcactcgca tattacattc
      181 acataatgta tataaacaat acttgttcac agaacatatt ttttaaatgc aattaattga
      241 caaactaaat cacatctaaa tacaatgtat aaaacaaaat aactatacat accaaacata
      301 caaacactta acatatgaat tgcataaaaa cattacccag tcgaacctcc gggtctgaaa
      361 gcccttgctt ttcagttatt agcaacattt aacatttata aattaacatc tggctgcgcc
      421 agagattacg tctctgagtg catcgttgtg cttgtagtat aataaataaa atggagtgtc
      481 attaagtgga taaggtttta gattcagccc tcctttcttc ggcaaataaa agaaagggta
      541 tccttgctat tggcgcacat cctgacgata tagaattagg ctgtggcgca tcgcttgctc
      601 gtcttgcgca aaaaggaatt tatatcgcag cagtggtgat gactaccggc aactctggta
      661 cagatggaat aatcgatcgc catgaagaat cgcgcaacgc cctaaagata ttagggtgca
      721 accaaactat tcatcttaat tttgctgaca cccgcgctca tttacagctc aatgatatga
      781 tttccgccct ggaagacatc attaaaaatc aaattccttc tgatgttgaa atcatgcggg
      841 tatataccat gcatgatgcc gaccgccatc aggatcatct cgctgtttat caagcttcaa
      901 tggttgcctg ccgcactatt ccacaaattc tcggctacga aaccccaagt acctggcttt
      961 catttatgcc tcaggttttt gaatccgtta aagaagaata tttcacagtc aaacttgctg
     1021 cattaaaaaa acataaaagc caggaacgac gtgattatat gcgtcatgat cgcctgcgtg
     1081 cagttgcaca atttcgcggg caacaggtca atagcgatct gggtgaaggc tttgtaattc
     1141 ataaaatgat tctttgagca aattgcatga aaacctggat atttatctgt atgtccatag
     1201 caatgttgct atggttttta agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta
     1261 ttgacgccat tatacctgcg tataacgaag gcccgtgtct ggcgcagtca ctggataatc
     1321 tactgcgtaa cccttatttt tgccgggtaa tttgcgttaa cgacggctcc acggacaata
     1381 ccgaagcggt catggcggaa gtcaaacgca aatggggcga ccgctttgtt gccgtcacgt
     1441 aaaaaaatac cggtaaaggt ggtgcgctga tgaatggcct caattatgcc acctgcgacc
     1501 aggttttttt aagtgatgcc gacacctatg ttccgcccga tcaagacgga atgggctata
     1561 tgctggcaga aattaagcgc ggtgctgatg ccgtaggcgg cattccctct actgcgttga
     1621 aaggcgcggg gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga
     1681 agcgcacgct acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa
     1741 tgttccgtac tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc
     1801 ttgatctcac ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca
     1861 tcgtataccc acaggaatgc aacagtccgc gtgaggagtg gcgtcgctgg cggcgttgga
     1921 ttgtgggcta cgcggtctgt atgcgcctgc ataaaaggct tttatttagc cgcttcggta
     1981 tcttcagtat atttcctatg ctgttggttg tgatttatgg cgttgggatt tatctcacta
     2041 cctggtttaa tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc
     2101 ttatctggat cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct
     2161 ggttgttggt gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct
     2221 ggattatcta tggacttatt gcctttttta ctggacgcga acctcagcgc gacaaaccca
     2281 cccgctattc cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa
     2341 ctgaaaaact atctgaagct taatgttaaa gagaaaaatt attcagattg cagaccaggt
     2401 aattattgat gaaactgcag gtgaagttgt tatcggcgcg aatacccgta tttgtcatgg
     2461 tgccgttatt cagggtccgg tagtgattgg cgcaaactgc ctgataggta attatgcgtt
     2521 tattcgtcct ggcacaataa tcagcaatgg tgtaaaaatt ggttttgcca ccgaaattaa
     2581 aaatgcggtt attgaagcgg aagcaacgat tggtccgcaa tgttatattg ccgactcggt
     2641 agttgcaaac caggcatatt tgggcgcaca agtacgtacc agtaatcatc gtctggatga
     2701 acagcctgtg tctgttcgaa ctccagaggg aattatcgct accggatgcg ataaattagg
     2761 ttgttatatc gggcagcgtt cacgccttgg cgtacaagtt attattttgc ctgggcgaat
     2821 tatttccccg aacacgcaac ttggcccgcg cgtgattgta gaacgtaatt tacctaccgg
     2881 aacttattca ctccgacaag aacttatccg tacaggagat taaaatgata aaacggacgt
     2941 tattagcggc ggccattttt agcgcattgc ccgcttatgc cgggttaact tccattaccg
     3001 cgggctacga ttttaccgat tattctggcg atcatggcaa ccgtaattta gcgtatgctg
     3061 aactggtggc gaaagttgaa aacgcaacgc tgctttttaa tctttcccag gggcgtcgtg
     3121 attatgaaac tgaacatttc aatgccactc gcggtcaggg tgcggtctgg tataaatgga
     3181 ataactggct gacaacccga acgggtattg cctttgcgga taatacgccg gtctttgccc
     3241 gccaggattt tcgtcaggat attaacctgg ccctgttgcc aaaaacgctt ttcacgaccg
     3301 gttatcgcta cactaaatat tacgatgatg tcgaagtcga tgcctggcaa ggcggcgtat
     3361 cactctatac tggcccggtg atcaccagct accgctatac ccattatgac tccagcgatg
     3421 caggtggtag ttatagcaat atgatttccg tgcgtctgaa tgacccgcgc ggcactggtt
     3481 atacgcaact atggctaagc cgcggaacag gcgcttacac ctatgactgg acgccagaaa
     3541 cacgctacgg cagcatgaag agcgtcagtt tgcaacgtat tcaaccgctg actgagcaac
     3601 ttaatctcgg gctgacggca ggtaaagtgt ggtacgacac cccaaccgat gattataacg
     3661 gtctgcaact tgcagcccat ctgacctgga aattctgatt gcttctgccg cccgctatcc
     3721 ggggcggcct tccctgccga ttagccccac tccctttcct cgttgttttc cgaccacatt
     3781 caccggataa attttattct ccagcgttat atactatagg gaggtatgca ttgacatata
     3841 gcatacctcc ctatagtata ttgcgtgcag atgatgaggt gcgaaatgcc cagtactccg
     3901 gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc gggggcagat tgatgctctg
     3961 gaacggtcgc tggagggtga tgccgaatgc cgtgccatac tccaacagat cgctgccgtt
     4021 cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa gccatatccg ggaaacgttt
     4081 gaccgaaatg actgctacag ccgcgaagtc agccaatccg ttgacgacac tattgaactg
     4141 gttcgagcct atcttaaata gctgaatcta ttaccatatt gaggaagagc gagagatgaa
     4201 atcacgtgct gccgttgcat ttgctcccgg taaaccgctg gaaatcgttg aaattgacgt
     4261 tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc cataccggcg tttgccatac
     4321 cgacgcattt accctctccg gggatgaccc ggaaggtgta ttcccggtgg ttctcggtca
     4381 cgaaggggcc ggcgttgtgg ttgaagtcgg tgaaggcgta accagcgtca aacctggcga
     4441 ccatgtgatc ccgctttaca ccgcggagtg cggcgagtgt gagttctgtc gttctggcaa
     4501 aactaacctc tgtgttgcgg ttcgcgaaac ccagggtaaa ggcttgatgc cagacggcac
     4561 cacccgtttt tcttacaacg ggcagccgct ttatcactac atggggtgct ctacattcag
     4621 tgaatacacc gtggtcgcgg aagtgtctct ggccaaaatt aatccagaag caaaccatga
     4681 acacgtctgc ctgctgggct gtggcgtgac caccggtatt ggcgcggtgc acaacacagc
     4741 taaagtccag ccaggtgatt ctgttgccgt gtttggtctt ggcgcgattg gtctggcagt
     4801 ggttcagggc gcgcgtcagg cgaaagcggg acggattatc gctatcgata ccaacccgaa
     4861 gaaattcgat ctggcgcgtc gcttcggtgc taccgactgc attaacccga atgactacga
     4921 caaaccgatt aaagatgtcc tgctggatat caacaaatgg ggtatcgacc atacctttga
     4981 atgcatcggt aacgtcaacg tgatgcgtgc ggcgctggaa agtgcgcacc gcggctgggg
     5041 tcagtcggtg atcatcgggg tggcaggttc tggtcaggaa atctccaccc gtccattcca
     5101 gttggtcact ggtcgcgtat ggaaaggttc cgcgtttggc ggcgtgaaag gtcgttccca
     5161 gttaccgggt atggttgaag atgcgatgaa aggtgatatc gatctggaac cgtttgtcac
     5221 gcataccatg agccttgatg aaattaatga cgccttcgac ctgatgcatg aaggcaaatc
     5281 cattcgaacc gtaattcgtt actgatttcc cgcaggtata ccccgtccac ttcagacggg
     5341 gttcttaata ctctccccgg gcagccgtcc gggggattaa ccgtgagata atgactgatg
     5401 gaactcattg aaaaacatgc cagctttggc ggctggcaaa atgtgtatcg gcattattcc
     5461 caatcactga aatgtgaaat gaatgtcggc gtctatctcc caccaaaagc cgcgaatgaa
     5521 aaattgccgg tgctgtactg gctttcaggc ctgacctgca acgagcagaa tttcattact
     5581 aaatcgggga tgcagcgtta cgcggctgag cacaacatta ttgttgttgc gccggacacc
     5641 agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg atctcgggca aggtgccggg
     5701 ttttacctga acgcaacgca agcgccgtgg aatgaacatt acaaaatgta tgactatatc
     5761 cgcaacgagc tgccggattt agtgatgcaa cattttccgg caacgaccag aaagtctatc
     5821 tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg cgttacgtaa cccagatgaa
     5881 tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat cgcaagtacc gtggggacag
     5941 caagcctttg ccgcatatct tggtgaaaat aaagatgcct ggttggatta cgatccggtg
     6001 agtcttattt cacaaggtca acgcgttgcg gaaatcatgg ttgatcaggg gttgagtgat
     6061 gatttttacg cggaacagct gcggactcca aatcttgaaa agatctgcca ggagatgaat
     6121 atcaagacgt taatccgtta tcacgagggt tacgaccaca gctattattt tgtctccagt
     6181 tttattggcg agcatattgc ctaccacgcc aataaactga atatgcgttg ataatagtgc
     6241 acgactgccg gatgcggcgt gaacgcctta tccggcctac acttcgcccg taaaccgtag
     6301 gcctgataag acgcgcaaag cgtcgcatca ggcatggcgc acgactgccg gatgcggcgt
     6361 gaacgcctta tccggtctac acttcgccca taaaccgtag gcctgataag acgcgcatag
     6421 cgtcgcatca ggcatctgcg cacgacttat ctatgtttac cacatcaaat catcaggcac
     6481 tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg gcactgagcg cgctgtgcaa
     6541 cacaatactg ctggcatcac gctgagcaat tttatcggca acactggcgg gaataatggt
     6601 gtattcacct tcgctattgt tatcgaccaa caagcgggca atcgccagac gaccattaat
     6661 caactgcgcc tgtgtgagct tatcgacaaa aatcttctta atcagattgc cgtcagtgaa
     6721 gttaaaacca atatcgccat tggcaatggt gattcggttc atttcaatga gctgcttcac
     6781 ctgggcttta tattctttcg ccaacgccgc ttgtttttgc tgttcgctaa gctgtttatc
     6841 acgctcaagc tgtgcctttt tattttcttc taccgcagcc cgagcttcac gcgcctgaac
     6901 gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt ttgctggtga ctaatcctgc
     6961 tttgagcaac tgctcttgta aggtaagttt tgccatcttt gtttccaaac ccgctgaata
     7021 atttttagcg agtatacctg taaacgccgt gggtgtattg ggggcaagtc cctttcgccc
     7081 ctgcttaata aggcgcgtca ggcatcggca cacggctgca ttcttgatct ccgactcatc
     7141 aaaataaaca ccgccaaccc agcaacaaga atacccggtg cagaagcggc cattacgccg
     7201 accgtgccag tgcctaatgc cagcattttc ccggccagta acggaccgct catagccccc
     7261 agacgcccta ccgccacggc tgttcccaca cctgttgcgc ggatctgcga actgtaaaac
     7321 aacagtgcca gggcatacaa aacgctttgc ccacctgtcg caaacaaccc cgcgacaaat
     7381 cccgccagca acataccgtt aaatgacgac accgttccaa gcgccagcag cgaagctaac
     7441 atgccgctat aaatcagtag cgacatggtt actggacgca gcttatccat caatgcgccc
     7501 aacattaacg tcccgcttgc cgcccccatt tgcagggcaa acatcacccc tgccgcctgc
     7561 gatggctgga atccttgctc caccaaaagt agcggtagcc agttgatcaa catataaacc
     7621 accagcagag tgaagaaata acacaaccac agcagcagcg tcgcggttgc cgtttctggc
     7681 gcaaataagg cacgcagtgg tggcgcagcc tgtttttcgc cagcgaaaac cgctgactcc
     7741 ggcagccagc gcattaatag cggcaccaga atcaacggca ccacaccacc tacccaaaac
     7801 accgtttgcc atgctaagtt tgcccccgcg aaacccagtg tcgccgccag cgccgcgcca
     7861 atgggaacac cgcaatacat caggctcact gccgtcccac gaaaacgtgg acccgcggct
     7921 tcagatgtca gggcgataag attcggcaac gccgccccca gcccgacacc ggtcatcagc
     7981 cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg ttgccagtga gaacaaacca
     8041 aacagcgcaa ctgagccaat caaaatacgc ttacgaccat aacggtctgc cagcatcccg
     8101 ccaaccaacg cgccgggtag caaaccgagt attccggcgc taaatatcca gcccatttgc
     8161 attttatcga gtgcgaaagc ctgggcgatg ccacccgccg caatgccagc cgcctgaaga
     8221 tccagccctt ccatcagagc gaccaaaaaa caaagcccga tggtcagcat caggcgggat
     8281 gaagatgatg aaggggtacg agtcgacata agatgaacct cattgcgagt gcaggtgaat
     8341 cgcgactgat acattgccag ccgcgtaatc agaactattt aatgactgcc tacgggcgaa
     8401 atgtagcgaa atgtaggccg gataaggcgt tcacgccgca tccggcagtc gtgcgcagat
     8461 gcctgatgcg acgctaacgc gtcttatcag acctacaaat tctcacgcca tccgtaggcc
     8521 ggacaaggcg ttcacgccgc atccggctgt cgtgctggga tgggcataca aaatgcacgt
     8581 tatcggcagt catcattatt tgttgttgcg cagatccagc gccacgtcga cgatcatatc
     8641 ctcctggccg ccaaccatcc ggcgtttgcc cagctcaacg agaatatcca ccgcacttaa
     8701 gccataacgc gccgccgccg tttcacagtg acgcaggaag ctcgagtaaa caccagcgta
     8761 tcccagcgcc agcgtttcgc gatcgactcg taccggacga tcctgcaacg gacgcaccag
     8821 gtcgtcggcg gcatccatta acgcatagag atcggtccca tgctgccagc ccagtttatc
     8881 cgcggcggca ataaacactt ccagcggtgc gttacctgcg cccgcgccca ttcccgcgag
     8941 gctggcgtcg attcggtcgc agccctcttc caccgccgcg atagagttcg ccacgccaag
     9001 actcaggtta tggtgagcgt gcatgccggt ttgcgtttct ggtttcaaca ctgctttcag
     9061 ggcgcggaaa cggtcacgga tatcgctcat gttcatcgca ccgccagaat ccaccacata
     9121 aatacaggtc gcaccgtagc cttccatcag ctttgcctgc ttggcgagat tctccggcgt
     9181 ggtcatatgg ctcatcatca gaaaaccaac ggtgtccatt ccgagctcgc gggcatactg
     9241 aatatgctgg gcggaaacat cagcttcggt acagtgcgtt gccacacgaa ccacccgcgc
     9301 gccagcctgc caggcatttt tcagatcgtg aatagtgccg attcctggca gcaacaacgt
     9361 cgcgattttg gcgtgcctca ccacatccgc cgccgcttca atccattcaa ggtcgctatg
     9421 tgcgccgaaa ccatagttaa agctggaacc ttgcaaaccg tcgccgtggg ccacttcaat
     9481 cgaatccacg cgggcatcgt ccagtgcttt ggcaatctgg cgaacgtttt ccagcgaata
     9541 ctgatgacga atggcgtgca taccgtcacg caatgtgacg tccgagatat aaagtttttt
     9601 accgttcatg ccgcttctcc tgccttgcgc gccagtgact gggccatttt ttccgctgtc
     9661 gccagcgcac tggaagtcat aatatcgagg ttgcccgcat aggcaggcag ataatgcgct
     9721 gcgccttcga cttccagcca gaccgctgtt ttcagtccgg agaattgccc cacgcccggt
     9781 aaattgaccg gtttatcctg cgggataact tcaaactgca cgcgctgttt caggcgataa
     9841 cccggtacgt aagcctgcac cgcctcagcc atttcattga ttgaggcttc gatatcatct
     9901 tgtgaagctt cgtcgctcaa tacatacacc gtgtcacgca tcatcaacgg tggctctgct
     9961 gggttaagca caataatcgc cttccctttt gccgcgccgc ccaccacttc aatggctcgg
    10021 gaagtggttt ccgtaaattc atcgatattg gcacgcgtgc caggtccggc agatttactg
    10081 gcgatagaag cgataatttc ggcgtaatga acacgcgcca cgcgtgaaac tgccgccacc
    10141 attggaatgg tggcctggcc gccgcaggtg accatgttga cgttcagttg atcgacgttc
    10201 gcctcgaggt taaccaccgg cacgcagtaa gggccgatgg cagcaggcgt caggtcaatt
    10261 aagcgaatat ccggtttcgc ttcgcgtaaa gcggcatcgt ttttcacatg cgcgcccgcg
    10321 ctggtcgcat caaatacaat gtcgatatca gcaaattcag gcatgttcat cagcccgatc
    10381 accccttcat gggtggtggc gacgcccata cgtctggcac gcgccagacc gtcggactga
    10441 ggatcaatgc cgaccatcac cgccatctcc agatgctgac cgtgacgcaa aattttaatc
    10501 atcagatcgg tgccaatgtt gccagaaccg ataatggcga ctttacgctt actcatgaca
    10561 gacttccttt tggggctgcg cttgaaaatg tcgcagcaac tgaacctatg ccttcaatat
    10621 gggcttcaaa acgatcgccc gcattcaccg ccaccatcgg gcctaatgcc ccggtaagaa
    10681 tgatatctcc ggcgcgcagc ggttcaccca gactggccat tttgcgtgcc agccagacgg
    10741 cggcattaag cggatgtccc aggcattcgc tgccgcgccc gctggaaacc tcttcgttat
    10801 tacgcgtcat cttcatggcg cagtttttca ggtctaaccc cgccggacgt tgcgccggac
    10861 cgccgatgac atacaccccg caggaagcgt tatctgccac ggtatcgaca aactgaatcg
    10921 accagtcgcg aatgcggctc cccaccactt ccagcgccgg aagtacccat tcaatggcgt
    10981 tatacagttc gtcgaaggtg atatcagttg cgggtaaatc gcggttcaac accagtgcga
    11041 tctccgcttc aatgcggggt tgcagaacac gggaaaaagg aatgatttcg ttatcgccat
    11101 aacacatgtc ggcaaataac gtcccaaaat ccggttgatc aacgcccagt tgttgttgca
    11161 ctttcggatg cgtcaggccc actttacgcc ctaccacgcg acgcccctgc acaacgtcat
    11221 attgcacatt tatgtgctga atggcgtagg ccgcttcagc gtgatcgata ccaatcagat
    11281 cgcgcagcgg cgcaatcgct tcgccctgct ctgcggcgcg gcgtaaatcc gccgccagtt
    11341 gctcaagagt atgcttcgtc atgaccattc cttaagggcg tgcgaggaaa ttcagcacca
    11401 attgattgaa agcgtcggca tgttcccact gcgcccagtg accacagtcg cggaagatat
    11461 gcagttcaga accggcaatg ccggacagca gacgcagacc cgcatccatc ggcacaaagc
    11521 ggtcgttgcg cccccagaca atcagggttt gcgctttgat ttccgccaga cgtgggccaa
    11581 aatccgggaa ctgtttcgga ttagcttcca ggctcttaac gaagttttcc aggtgatcgc
    11641 ggcgcgacag catattattc aggcgcgctt caaacagggc gtcggtcaaa tcgctggtat
    11701 cgaaaacgaa gatatccatc atcagcttca ggttttcgat agtcggctga cgataaagct
    11761 gattcagtcg cttaatacct tcggttggca tcggcgtaaa caaactcatg ccgcccgtac
    11821 cgccgcccat cagcaccagt ttgccgacgc gctccggcca gttaagggtg aacgcca
//
LOCUS       DAZPAC010000123        11807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZPAC010000123 DAZPAC010000000
VERSION     DAZPAC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.123"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..487
                     /locus_tag="ACHUPM_002795"
     CDS             <1..487
                     /locus_tag="ACHUPM_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0870523.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            539..1474
                     /gene="ttcA"
                     /locus_tag="ACHUPM_002796"
     CDS             539..1474
                     /gene="ttcA"
                     /locus_tag="ACHUPM_002796"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR0870524.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="ACHUPM_002797"
     CDS             complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="ACHUPM_002797"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR0870525.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3006..3179)
                     /gene="ynaL"
                     /locus_tag="ACHUPM_002798"
     CDS             complement(3006..3179)
                     /gene="ynaL"
                     /locus_tag="ACHUPM_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR0870526.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="ACHUPM_002799"
     CDS             complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="ACHUPM_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR0870527.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4451..4498)
                     /gene="ydaN"
                     /locus_tag="ACHUPM_002800"
     CDS             complement(4451..4498)
                     /gene="ydaN"
                     /locus_tag="ACHUPM_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR0870528.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4692..5924
                     /gene="dgcM"
                     /locus_tag="ACHUPM_002801"
     CDS             4692..5924
                     /gene="dgcM"
                     /locus_tag="ACHUPM_002801"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR0870529.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="ACHUPM_002802"
     CDS             complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="ACHUPM_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFR0870530.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6838..7746)
                     /locus_tag="ACHUPM_002803"
     CDS             complement(6838..7746)
                     /locus_tag="ACHUPM_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0870531.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7922..9232
                     /gene="abgA"
                     /locus_tag="ACHUPM_002804"
     CDS             7922..9232
                     /gene="abgA"
                     /locus_tag="ACHUPM_002804"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFR0870532.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9232..10677
                     /gene="abgB"
                     /locus_tag="ACHUPM_002805"
     CDS             9232..10677
                     /gene="abgB"
                     /locus_tag="ACHUPM_002805"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR0870533.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10714..>11807
                     /locus_tag="ACHUPM_002806"
     CDS             10714..>11807
                     /locus_tag="ACHUPM_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR0870534.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
BASE COUNT         3005 a         2999 c         2927 g         2876 t
ORIGIN      
        1 gaatgtcaga agaaacctga cgatacttga tatgttcggt cccccaaaaa caaatgccgg
       61 gatccggacg gtaacattac tgcagccggc tcttgaagca ctgaaggagc aatacaaact
      121 gaccgggcat catcgcaaaa gcgaaatcac tttttatcat cgggagtacg gcagaactga
      181 aaagcaaaaa ctgcattttg ttttcatgcc cagggtgtgt aacgaaaaac agaaacctta
      241 ttactcggta agcagtttgg gtgcaagatg gaatgcagca gtaaaacgtg ctggtattcg
      301 ccgccgtaat ccgtaccata cgcgacatac ttttgcctgc tggctgttga cggcaggagc
      361 gaacccggca tttatagcca gccagatggg gcatgaaact gcgcaaatgg tgtatgaaat
      421 ttacggtatg tggattgatg acatgaacga cgaacagata gccatgttga atgcgcggtt
      481 atcgtagttg caaagtttgc ccccaatttg ccccatttag taccagagaa ctgaaataat
      541 gcaagaaaat caacaaatta caaagaaaga acaatacaac ctgaacaaat tacaaaaacg
      601 tctgcgtcgt aacgtgggcg aagccattgc tgacttcaat atgattgaag aaggcgatcg
      661 catcatggtt tgcctctccg ggggtaaaga cagctatacc atgctggaga ttctgcgcaa
      721 tttgcagcaa agcgcgccaa tcaatttttc gctggtggct gttaacctcg atcaaaagca
      781 accgggcttc ccggaacacg ttctgcccga gtatcttgaa aagctgggcg ttgagtacaa
      841 gattgtcgaa gagaacactt acggtatcgt gaaagagaag attccagagg gcaaaaccac
      901 ttgctcactg tgttctcgcc ttcgtcgcgg tatcctttat cgtaccgcaa cggaactggg
      961 ggcgacgaag atcgcgctgg gtcaccatcg tgacgatatc ctgcagacgc tgttcttaaa
     1021 tatgttctac ggcggcaaga tgaaaggtat gcctccgaaa ctgatgagcg atgatggcaa
     1081 acatatcgtt attcgtccgc tggcctactg ccgcgagaaa gatattcagc gatttgccga
     1141 tgcaaaagcg ttcccgatta ttccgtgcaa cctgtgcggt tcacagccta acctgcaacg
     1201 tcaggtgatt gctgacatgt tgcgtgactg ggataaacgt tatccagggc gtatcgagac
     1261 gatgttcagc gcgatgcaga atgtggtgcc gtcgcatctg tgcgatacca acctgttcga
     1321 tttcaaaggc atcacccacg gttctgaagt ggttaacggg ggtgatctgg cgtttgatcg
     1381 cgaagagatc ccactacaac cggcgggctg gcagccagaa gaagatgaaa atcagttgga
     1441 tgagttacgg ctgaatgtgg ttgaagtgaa ataaccagga tggcgcccga tgcgcaagcg
     1501 tatcgggcta ctcttatgga ggccggataa gacgcggcca gcgtcgcatc cggcaatccc
     1561 gaataagatg tttactcttg cacccggcaa ttcaacattt cattatttta ataaccgcac
     1621 ccggctcgtt tttcctttaa tcttcccgcc ctgtaactgt ttccatgctt tatgagcaac
     1681 agcctgacgg accgcgacat agacatgcgc cggatgcacg gcgattttgc caatatctgc
     1741 gccatcaagc ccgatatctc ctgtcagtgc acctaataca tcacccgggc gcattttggc
     1801 ttttttccca ccatcgatac acaacgttgc catttctgct tccagcggca caatggaact
     1861 attagctggc ggcgtttgcc agttaagttt tatctgcaac atgtcagaaa tgatattggc
     1921 ccgctgtgct tcttccggag cacagaaact gatcgccaga ccgctatttc ctgcacgagc
     1981 tgtacggccg atgcgatgta catgaacttc agggtcccac gccagctcaa agttcaccac
     2041 cagctcaagc gatttaatat ccagaccacg cgcagcaaca tcagtcgcaa ccagtacacg
     2101 ggcgctaccg ttagcaaaac gtaccagggt ctgatcgcga tcgcgttgct ccaaatcgcc
     2161 gtgtaatgac aatgcacttt gccctacttc attcagcgcg tcgcagacag cctggcaatc
     2221 ttttttggta ttgcaaaaca ccacgcaaga ggatggctga tgcaagctta ataaccgttg
     2281 caacagagga attttgcctt tgctggatgt ctcataaaat tgttgttcaa tgggtggcaa
     2341 agcatctgtt gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattac
     2401 ggcaatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa
     2461 acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc
     2521 catcaccaaa gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa
     2581 acgacccggc gttgccacga taatatgcgg cgcatgttgc aacgaatcac gctgcatacc
     2641 gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg
     2701 acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg
     2761 ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt
     2821 gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc
     2881 ggcggcctgc accggcgtca tagttaaata acccaactca ttaaggttcg tgagttgggc
     2941 gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg
     3001 caaagttagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt
     3061 tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga
     3121 acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata
     3181 tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa
     3241 aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa
     3301 aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa
     3361 gggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt
     3421 tttgtataca gacagccgga aaaattgctt ttgttacaac catttactac ggtgcaacca
     3481 taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg
     3541 ccacccgccg ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact
     3601 gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg
     3661 catcacctga gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc
     3721 gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct
     3781 catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata
     3841 gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg
     3901 ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc
     3961 gctggaatga tcggtcaacg catcgcacac atccaccagc caccctccgc aatcggtcgg
     4021 acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg
     4081 ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg
     4141 ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgagacggct
     4201 gactcggggc cgcgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg
     4261 tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg
     4321 atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag
     4381 catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccacagt
     4441 gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct
     4501 catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca
     4561 aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat
     4621 tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata
     4681 acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct
     4741 ggatcgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga
     4801 tgcaagacct cacgtttagt cagctacgaa ccggtcccta ttccgtctcc tcacaaaaag
     4861 aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg
     4921 ttcagcgtaa agaagaggaa acagcattaa actgtcggct tgttttgcga aagctgacag
     4981 aagcagaacc ggtgattatt ttcgaaggta tcgaagcgtc ggcaacgctg ggtttaaaag
     5041 ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga
     5101 ctaactctgc accaatgttg ttgattgacc cctcacgaga tggccaaatc gtcgatgcta
     5161 acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct
     5221 gggaaataaa tatgctcggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac
     5281 ccggtggtca taaacctctg aattttattc ataagctggc ggatggttcg actcgtcatg
     5341 tgcagaccta cgccgggccg attgaaattt atggcgacaa gctcatgtta tgtattgtac
     5401 atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgcc caccatgacg
     5461 cgatgaccgg attactgaat cggcgacagt tttatcacat taccgagcca ggccaaatgc
     5521 agcatctcgc catcgcccag gattacagct tgttgctcat cgacaccgat cgttttaaac
     5581 acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgca
     5641 ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg
     5701 tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg
     5761 taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg
     5821 cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt
     5881 atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc
     5941 gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt
     6001 tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgca gtgcaatatg
     6061 cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat
     6121 gcgatttatc atcccgtcct ttgccatgaa taatcagcac gttacgcaaa ccatcagcca
     6181 tggcttgttg aatgaaactg aacatcattt tgcggcactc ttccaccggc tggcgcaaaa
     6241 gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgtt
     6301 gcaacccttc ccgccgaaac tccagcggct gacttagtgg gatgatgtcg agaaatccgg
     6361 tggtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg
     6421 ttggatgcca gtggacatcg gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa
     6481 gaaacagcga tttgtcgtca aggttcatgt tacatcctcc cgcaattaag agcgcgatat
     6541 gataaccaga ccgggtcggt ccaacaacgt attacccaaa attccagtaa taagttccaa
     6601 atattgccga tattttaagc aaaatgctta tgcatgatta ttcattcacg atattaataa
     6661 tgtaacttat attttcgtga aatctgtcac tgaagaaaat tggcaactaa aggttaaaac
     6721 cgttataaca cagtcaccgg cgcagaggag acaatgccgg atttaagacg cggatgcact
     6781 gctgtgtgta ctgtagagtc tggcggatgt cgacagactc tattttttta tgcagtttta
     6841 actttgcaga tagccgcatt ctcgccggaa ttgcgtgatt aatgacgcgg tcagtggtgt
     6901 ctggcgacta tcacgccgct gaatcaaata ataggccgct ttcggtaaaa tttcgctaac
     6961 cggcaacatc accagcccct gtccgtgcaa gggatcgcag cccatttctt caggcagtat
     7021 gctgagaaaa tcgctttttg ccaccagact gatacaggct gagaacgtct cgcagactac
     7081 accgacctgt ggcgtttgcg cctgatcgtc aagcaattca ctcaactgtt tgtagtaact
     7141 gccgtgtggc gtcggcattg tccagctgta atccagtaac tgtttgatcg aacgggcacc
     7201 aatggcgggg tgtcccgggc ggcaaaagat cgcgaattgc ttttccagta atttctcaaa
     7261 agtaaattcg tggtcgtacg gtccctgata ataggtattg atggtgaaat ccaattctcc
     7321 ctgacgcaat tcattaatca tcgacaccag ttgcccttcc ataatgcgta cttttacctg
     7381 cggatgctgc tgatgaaaac gagatatgac agctggcatc agactgcggg aaatactggc
     7441 ccccatgccg atattaatct gccctgccag ttgcccttgt cgttggcgaa tatcctcttg
     7501 ggctgcgcgc agctcttcaa gaataagact ggcgtgctga taaaaacttt caccggcatc
     7561 agttaacgtc acgcctttac tacggcgaaa aaagagttgc gccgctaatc cttcttccag
     7621 ctcctgaata gatttactca gtgccggttg cgacatattc aacattcggc tcgctccgcg
     7681 aatgctgccc tgacgagcca cttcaacaaa agcccgaatt tgatgaattt ttacctgaaa
     7741 agccatgacg ccaccgataa ccgttattta tcagaccaaa gaaactggca tctactttaa
     7801 tgcagatgat tgtgtcaggg taatttatga acggttaaaa ctgtgaaaaa tcagttagtg
     7861 ataagtaaaa actatcgcta cgtgaaccgg gtcacacttt ttactgatga cgggaaaggt
     7921 tatggagtct ttgaatcaat ttgttaattc gcttgcccca aaattatcgc actggcgacg
     7981 tgattttcat cactatgcag agtctggctg ggtggaattc cgcactgcca cccttgttgc
     8041 ggaagaattg cagcagctcg gctattcact ggcgctgggc cgcgaagtag ttaatgaaag
     8101 tagccggatg ggactacctg atgaactcac tctacaacgc gaattcgagc gcgctcgtca
     8161 acagggtgcg ctagcacaat ggattgcggt ttttgaaggt ggtttcactg gtatcgtcgc
     8221 caccctggat accggtcgcc ccggtccggt gatggctttc cgtgtcgata tggacgcgct
     8281 ggatctcagt gaagagcagg atgtcagcca tcgcccctac cgcgacggtt ttgcgtcatg
     8341 taacgccgga atgatgcatg cctgtggtca tgatggacat accgccattg ggcttgggct
     8401 ggcgcatacc cttaaacaat tcgagtccgg actacatggc gtcatcaaac tgatttttca
     8461 gcctgcagag gaaggtacgc gtggcgcgcg ggcgatggtc gatgcaggtg tcgtagatga
     8521 tgttgattat tttactgccg tgcacattgg cactggcgta cctgcgggca ccgtggtgtg
     8581 cggcagtgat aattttatgg caaccaccaa atttgacgcg cacttcaccg gtaccgccgc
     8641 tcacgcaggc gcaaaaccag aagacggtca caatgccttg ttggcggcag cacaagccac
     8701 tcttgcactg catgcaatcg ccccgcacag cgaaggagct tccagagtaa acgtgggcgt
     8761 tatgcaggca ggaagcggtc gtaacgttgt tcctgcctcg gcgttgctga aagtggaaac
     8821 acgcggggcc agcgacgtca ttaatcaata tgtttttgac cgtgcacaac aagcgattca
     8881 gggcgcagca accatgtatg gtgtcggcgt tgaaactcgt ctgatgggtg cagttaccgc
     8941 cagttctcct tcgccgcaat gggtcgcatg gttgcaaagt caggcggctc aggtcgcggg
     9001 ggtcaatcag gccattgaac gtgttgaagc gcctgcgggt tccgaagatg ccacattaat
     9061 gatggcccgc gtgcagcaac atcaagggca agcctcctac gtggtgtttg gcacacagct
     9121 ggcggcaggt catcacaacg aaaaattcga ttttgacgag caggttctcg ctattgccgt
     9181 cgaaacgctg gcgcgcaccg cgctcaattt tccctggacg cgaggtatct gatgcaggaa
     9241 atctatcgtt ttatcgacga tgcgattgaa gccgatcgcc aacgttatac cgatattgcc
     9301 gatcaaatct gggatcatcc agaaacacgt tttgaagagt tctggtcagc ggagcatctg
     9361 gcttcggctc tggaatctgc aggcttcacc gttacccgca acgtaggcaa tatcccaaat
     9421 gcctttattg cttcgtttgg tcaaggcaaa ccggttatcg ccctgctggg agaatatgac
     9481 gccctggcag gtttaagtca gcaagctggt tgcgcgcaac ctacatccgc gacgcccggt
     9541 gaaaatggtc acggttgcgg acacaatttg cggggaaccg ccgcctttgc cgctgcaata
     9601 gccgtcaaga aatggctgga acaatatggg caaggcggca cagtgcgctt ttatggttgt
     9661 cctggcgaag aaggcggctc gggtaaaacg ttcatggtcc gcgagggggt atttgatgat
     9721 gtggatgcgg cactcacctg gcacccggaa gcctttgccg gtatgttcaa tacccgcacg
     9781 ctggcaaaca ttcaggcatc atggcgcttt aaagggatcg cagcacatgc cgcgaattcc
     9841 cctcatttgg gacgcagcgc ccttgatgcc gtaacgttga tgaccactgg caccaacttc
     9901 ctcaacgaac atattattga aaaagcgcgc gtacactatg ccatcacaaa tagcggcggg
     9961 atctcgccca acgtggtcca ggcgcaggca gaagtgcttt atcttatccg cgcccccgaa
    10021 atgaccgacg tgcagcatat ttatgatcgg gtcgccaaaa tcgccgaagg tgcggcattg
    10081 atgaccgaaa ccacggttga atgccgcttc gacaaagcct gttccagtta tctcccgaat
    10141 cgcaccttag aaaatgccat gtaccatgcc ctatcccatt ttggtacccc ggaatggaac
    10201 tccgaagaac tggcttttgc gaaacaaatt caggctacgc tcacccccaa cgatcggcaa
    10261 aacagtctga ataatatcgc cgcaaccggt ggcgaaaacg gcaaggcttt tgcactacgt
    10321 catcgtgaaa cggtactggc gaatgaggtc gctccatatg ccgccaccga taacgtgctt
    10381 gcggcatcga ctgatgtcgg cgacgttagt tggaaactgc ctgttgccca gtgtttcagc
    10441 ccctgttttg ccgtcggtac accgctacat acgtggcaac tggttagcca ggggcgaaca
    10501 tctattgctc ataaaggaat gctgctggcg gcgaaaacta tggcagcaac cacagtcaat
    10561 ctcttcattg attcagggct attgcaagaa tgccaacaag agcatcagca agtaacggac
    10621 acgcaaccgt atcactgccc tatcccgaaa aacgtgacac cgtcaccttt aaaataacaa
    10681 caacaacgca aacacaacaa ccgaggaatg cccatgagta tgtcatccat accgtcgtcc
    10741 tcccaatccg ggaagctcta tggctgggtc gaaagaattg gtaacaaggt tctccatcct
    10801 tttttgctct ttatctattt gattatcgta ctcatggtga cgacggcaat tttgtcggcc
    10861 tttggcgtca gtgcgaaaaa cccgaccgat ggaacgccgg tggtggtgaa aaacctgctc
    10921 agtgtggaag gattacactg gtctttaccc aatgttatta aaaactttag cggttttgct
    10981 ccacttggtg cgatcctggc gctggtttta ggtgccggtc tggcggagcg cgtcggctta
    11041 ctgccagcgc taatggttaa aatggcatcg catgttaatg cccgctacgc cagttatatg
    11101 gtgctgttta ttgctttttt cagccacatt tcttccgatg cgacgttagt gatcatgcta
    11161 ccgatgggtg cgctgatttt tctggcggtg ggcaggcatc cagttgcagg tttactggct
    11221 gccattgcag gcgtaggttg cggctttacg gctaatttac tgattgtcac aaccgacgtg
    11281 ttgctgtcgg ggatcagcac ggaagcggcg gctgcgttca atccgcaaat gcacgtcagt
    11341 gtaattgata actggtattt tatggccagc tccgtagtcg tactgacgat tgttggcggc
    11401 ctgataaccg acaaaatcat cgagccacgg ttaggtcaat ggcagggaaa cagcgatgag
    11461 aaactgcaga cattgaccga aagtcagcgt tttggtttac gcatagcagg tgtcgtatcg
    11521 ctacttttta ttgctgcgat tgcgctgatg gtgatcccgg aaaacgggat attgcgcgat
    11581 ccgattaatc acaccgtgat gccatcaccc tttattaaag gtatcgtgcc actgatcatt
    11641 ctttttttct ttgttgtctc gctggcttat ggcatcgcta cccgcacaat tcgacgtcag
    11701 gcggatttac cgcagttaat gattgaaccg atgaaagaga tggcaggatt tatcgtgatg
    11761 gtttttcccc tcgcccagtt tgtcgccatg tttaactgga gcaacat
//
LOCUS       DAZPAC010000124        11693 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZPAC010000124 DAZPAC010000000
VERSION     DAZPAC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11693
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.124"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(48..869)
                     /gene="bacA"
                     /locus_tag="ACHUPM_002807"
     CDS             complement(48..869)
                     /gene="bacA"
                     /locus_tag="ACHUPM_002807"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0870535.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(960..1328)
                     /gene="folB"
                     /locus_tag="ACHUPM_002808"
     CDS             complement(960..1328)
                     /gene="folB"
                     /locus_tag="ACHUPM_002808"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR0870536.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            1433..2050
                     /gene="plsY"
                     /locus_tag="ACHUPM_002809"
     CDS             1433..2050
                     /gene="plsY"
                     /locus_tag="ACHUPM_002809"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR0870537.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWSVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(2063..2995)
                     /gene="ttdR"
                     /locus_tag="ACHUPM_002810"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(2063..2995)
                     /gene="ttdR"
                     /locus_tag="ACHUPM_002810"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
     gene            3202..4113
                     /gene="ttdA"
                     /locus_tag="ACHUPM_002811"
     CDS             3202..4113
                     /gene="ttdA"
                     /locus_tag="ACHUPM_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFR0870538.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            4110..4715
                     /gene="ttdB"
                     /locus_tag="ACHUPM_002812"
     CDS             4110..4715
                     /gene="ttdB"
                     /locus_tag="ACHUPM_002812"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR0870539.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            4764..6227
                     /gene="ttdT"
                     /locus_tag="ACHUPM_002813"
     CDS             4764..6227
                     /gene="ttdT"
                     /locus_tag="ACHUPM_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR0870540.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(6270..7283)
                     /gene="tsaD"
                     /locus_tag="ACHUPM_002814"
     CDS             complement(6270..7283)
                     /gene="tsaD"
                     /locus_tag="ACHUPM_002814"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR0870541.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            7520..7735
                     /gene="rpsU"
                     /locus_tag="ACHUPM_002815"
     CDS             7520..7735
                     /gene="rpsU"
                     /locus_tag="ACHUPM_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR0870542.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            7846..9591
                     /gene="dnaG"
                     /locus_tag="ACHUPM_002816"
     CDS             7846..9591
                     /gene="dnaG"
                     /locus_tag="ACHUPM_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR0870543.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            9786..11627
                     /gene="rpoD"
                     /locus_tag="ACHUPM_002817"
     CDS             9786..11627
                     /gene="rpoD"
                     /locus_tag="ACHUPM_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR0870544.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2851 a         3164 c         3010 g         2668 t
ORIGIN      
        1 tacagagcga gggagaaaac caggcctgct cccgaacctt ttctcaatca aaagaacacg
       61 acgtacaccg cagccgccac aataaagcga taaatggcga acgggataaa cgaaatgcgc
      121 ttaatcaatt gcaggaaggt tttaatcgct atcagcgcca ccacaaaagc ggtgataaac
      181 ccaacggcaa acatcgagat atcgccggtt gtcaggaagc cccagctttt gtagagatcg
      241 agcgccgttg cgcccatcat catcggtacc gccagcagga acgagaactc ggaagcagcg
      301 taacggctca cgcccatcag catcccacct gaaatggtgg ccccggaacg ggagaaaccc
      361 ggccacagcg ccagacactg gaaacagcca atcataaacg cctgacgata ggtcatatca
      421 tcaagacccg gcgcacgcgg ctctttcggc ttcaggcatt cggcggcaat cagcaacaaa
      481 ccgccaacga ccagtgcata catcacattt atcgggttaa acaatgactt aatcgtgtcg
      541 tggaacaaca gccccagtac caccgccgga atcatcccca gcaaaatgtg gatcagcgtt
      601 aaacgacctt tgctttctcc ttcgtgctgc aacgggcggc caaagtggat gccaatcagg
      661 ccaaacagac gccgccagaa catcactact accgccagaa ttgatcctaa ctggatcaca
      721 acttcaaagg ttttcgccgt gtcgccctcg aaccccaaca agtgaccgac aataatcata
      781 tggcccgtgc tggataccgg cagaaattct gtcaatcctt cgaccacacc caatattgcc
      841 gctatcagca gcgagtgcat atcgctcatc aataaacccc taaattatta aaatgtaccg
      901 cttgtccgga ctactgcagt aagaccaggt tataaccgtt tggtttaaca gctgtaaaat
      961 taattatttt ctttcagatt attgccacgc tcaatgatta caccaacatt cgccgcccgc
     1021 gccattgcgc ctggcttgct gagtttgatg cgcacccacg gcgagttgaa gcgtgctaac
     1081 agcagctccg ccacctcttc agccacgcgt tccaccagcg caaaacgcgc cccctcgacg
     1141 tggctgacca ccgtttctgc aatgtcagcg taactgaggc aatccgccac atcatcactt
     1201 ttcgccgctt tacggttatc ccatgccatt tcgatatcga acactaactt ctgttcgatg
     1261 gtctgttccc agtcgtaaac accaatagtg gtgattaccg aaagttgctc tataaataca
     1321 atatccatca cgtcctgcct gctttttggc taaccggata ccacttccgg cgaaatgtgc
     1381 gtattatcca cagattcatc gttgaacacg aattttcaaa acggaacagc ttatgagtgc
     1441 aatcgcgcct ggaatgatcc tcatcgcgta cctctgcggc tccatttcca gtgccattct
     1501 ggtttgccgc ttgtgtgggc tgcccgatcc gcgaaccagc ggctccggca atccaggcgc
     1561 aaccaatgtg ttacgtatcg gtggcaaggg agcagccgta gcagtactga ttttcgacgt
     1621 tctgaaagga atgttgcccg tctggggcgc gtatgaatta ggtgtcagcc ccttctggtt
     1681 aggcttaatt gccatcgccg cctgtcttgg acacatctgg tccgttttct tcggatttaa
     1741 aggaggaaaa ggcgttgcta ccgcttttgg tgccatcgca cccattggct gggatctcac
     1801 cggagtaatg gcgggaacct ggttactgac cgtgctattg agcggatact cgtcgctggg
     1861 agcgattgtc agtgcactga ttgctccgtt ttatgtctgg tggtttaagc cacaattcac
     1921 cttcccggtt tcgatgctct cttgcctgat cctgctgcgt catcatgaca acatccagcg
     1981 tctgtggcgt cgtcaggaga caaaaatctg gacgaaattc aaaagaaagc gcgaaaagga
     2041 tcccgagtga tttctggtgg atctacatga cctgatagcc ttcatcgggc ttgcccagcc
     2101 gttgctggca ccatgccgcc agaaattcca cgcagacacg taatttcatg ctgcgataga
     2161 gcggctcccg gtaaacagcc cagatattgg cgctttgtgc atactccggc aatacttgca
     2221 ccagtttgcc actctccaga aacggcaaca catcccactc ggaacgcagc ataatccctt
     2281 tgccctccag cgcccattgc aacactatct cgccgctatt ggaggaaaga tgcccgctaa
     2341 ccttcaccga ttttttctcc tgaccgttcc ccaactccca tattccgtgg gtcatatcgc
     2401 gctctttggt caccagacag tcatgacgac ttaattcctg taatgattgt ggctgcggat
     2461 atttttgcag atactcaggt gctgcgcata atattctttt atttttcgtt aacagatgcg
     2521 caatataata atcaggaatt tcgtcattaa tacgaatatc cagatcaata ttatcctgca
     2581 ctaaatcaat ttgccgatcg aacaattcaa aatgcacctg taactcagga taattgcgca
     2641 taagttcggt aatggctggc gcaatatggc tgcgcccaaa accaaaactg caaccaatac
     2701 gaatcatccc ctccgggcgc gttttgattt acgtgacgtc atcgaccagt cgctgatatt
     2761 gggtgaggat ttccagcgca tgttcgtaac aacgttggcc actttcagtc agcgccacgc
     2821 cgcgagcgga gcggttcagc agcgttgttg ccagcgtatt ttcaaggatt tggatccgct
     2881 tggtgacaaa tgcaggcgtc tgcccaagcg tcgccgctgc ggcgctaaaa ctcccgctgt
     2941 ggacgatttc taccagtacc tgcaagtctt tggctaaagg ccagctattc agcatcgtta
     3001 attatccgca gttgtgataa gcgcagtgta tttcgcaaaa cattgccatt ccctgctctg
     3061 gcgcaatatt cattcttatt ttcgcgttga ttcacgaatt gttaattaca tattcacggt
     3121 ggcaaaaaat ataaaaccac atttttagtg gtagtttgtg gcggtgaatt tttccagaca
     3181 aatacaaaaa ctggagttgc catgatgagc gaaagtaata agcaacaggc agtgaataag
     3241 ttgacggaga ttgtcgctaa ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg
     3301 gtggataaac taaaacagct aaaggatgcc gaaacgtcgt cgatggggaa aattatctac
     3361 catacgatgt tcgacaacat gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac
     3421 accggggaga ttatgttctt cgttaaagtc ggttcccgct tcccactgct tggcgagctg
     3481 caaagcatac tcaaacaagc cgtggaagag gcaaccgtca aagcgccact acgtcacaat
     3541 gcggtagaaa tttttgacga agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg
     3601 gtcacctggg acatcatccc cgacaatgac gatgcggaaa tcgaagttta catggcaggc
     3661 ggcggctgca cgctacctgg ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc
     3721 gtggtgaaat tcgtcttcga aaatatctcc accctcgccg taaacgcctg tccaccggta
     3781 ctggtgggcg tgggcatcgc cacctcggtg gaaaccgccg ccgtactctc gcgtaaagcc
     3841 attttgcgcc cgattggcag ccgccatccc aatccaaaag cggcagaact ggagctacgc
     3901 ctggaagaag gactcaaccg tctggggatt ggtccacaag ggctgactgg caacagttca
     3961 gtgatgggcg tacatatcga atctgccgcc cgccatccgt caaccatcgg cgttgctgtc
     4021 tctaccggct gctgggcgca tcgtcgcggc acactgctgg ttcatgccga tctcaccttt
     4081 gaaaatctgt ctcacacccg gagcgcgtta tgaaaaagat cctgacaacc ccgatcaaag
     4141 ctgaagatct gcaagatatt cgcgtcgacg atgtgatcta cctgaccggt acgctggtga
     4201 cctgccgtga cgtttgtcac cgccgcttga tcgaactgaa acgtccgatc ccttacgatc
     4261 tcaacggcaa agcgattttc cacgctggcc ccatcgtgcg caaaaacggc gataaatggg
     4321 agatggtttc cgttggcccg acaaccagta tgcgtatgga aagttttgaa cgtgaattta
     4381 tcgagcagac cggcgtgaaa ctggtggttg gcaaaggcgg aatggggccg ctgaccgaag
     4441 aaggctgcca gaaattcaag gcgctacatg tgattttccc ggcaggctgc gcggtgctgg
     4501 cggcaaccca ggtggaagag attgaagaag tgcactggac agagctcgga atgccggagt
     4561 cgctgtgggt ctgtcgggtc aaagagttcg gcccgctgat tgtctctatt gatacccacg
     4621 gcaacaacct gatagccgaa aacaaaaagc tgttcgccga acgccgcgat cccatcgtgg
     4681 aagagatctg cgagcacgtc cattacatca aataaccctc ccggagaggc tcgccccctc
     4741 tcctttttcg caggcataac acgatgaaac cttccactga atggtggcga taccttgcgc
     4801 cgctggcggt catcgccatt attgctctaa ttccggttcc cgcagggctg gagagtcata
     4861 cctggctcta ctttgccgtt tttactggcg tgatcgttgg actgatcctc gaacccgtgc
     4921 cgggtgccgt ggtggcgatg gtgggtatat ccatcatcgc catactctct ccctggctgc
     4981 tgttcagccc ggagcagctc gctcagccag gctttaaatt cactgcaaaa tccctctcgt
     5041 gggccgtttc cggtttttct aattcggtta tctggctgat tttcgccgcc tttatgtttg
     5101 gcacaggcta tgaaaaaacc gggcttggac gccgcattgc gctgattctg gtgaaaaaga
     5161 tgggacatcg cacgctgttt ctcggctatg cggtgatgtt ctccgagctg atcctcgcac
     5221 ctgtaacacc gtccaactcg gcgcgtggtg cggggattat ctatcccatc atccgtaacc
     5281 tgccaccgct ctatcaatca caaccaaacg acagcagttc gcgcagcatt ggctcgtaca
     5341 tcatgtggat ggggattgtt gccgactgcg tgaccagcgc cattttcttg acggcgatgg
     5401 caccaaactt gctgttaatt ggactgatga aaagcgcatc tcacgccacg ctgagttggg
     5461 gcgactggtt cctcgggatg ttgccgctca gtattttgct ggtgctgctg gttccctggc
     5521 tggcttatgt gctgtacccg ccggtactga agtctggcga tcaggtgccg cgctgggcag
     5581 agacggaact gcaggcaatg ggcccgctct gttcgcgtga aaaacggatg ctggggctga
     5641 tagtaggcgc gctggtgctg tggattttcg gcggtgatta tatcgatgcc gcgatggtcg
     5701 gttacagcgt ggtggcactg atgctgcttc tgcgcattat cagttgggac gacattgtca
     5761 gtaataaagc ggcgtggaac gttttcttct ggctggcctc gcttatcacc ctcgctaccg
     5821 gactcaacaa caccggtttt attagctggt ttggcaaact gttagcaggc agcttaagcg
     5881 gttattcgcc aacgatggtg atggtggcgt tgattgtggt gttttatctg ctgcgctact
     5941 ttttcgccag cgccacggcg tatacctccg ccctcgcacc gatgatgatt gccgctgcgc
     6001 tggcgatgcc ggaaatcccg ctgccggtgt tctgcctgat ggttggcgcg gcaattggtc
     6061 tggggagcat tcttacacca tacgccaccg gacccagtcc gatttactac ggtagtggtt
     6121 atctgccaac ggcggattac tggcgactgg gggcgatttt tgggctgata ttcctcgtgt
     6181 tgctggtgat taccggctta ctgtggatgc ccgtggtgtt gctttaagtg aggacggtgc
     6241 cggaaattgc tctccggcac ccaatagttt tacgcagccg gtaactccgc cagcggccag
     6301 cgcggacgca cgctaacgcc gagatccgcc gtcgcgcctg ctttaaaccg caccattccg
     6361 gcataggcga tcatcgcgcc gttatcagta caaaactccg gacgcgcgta gaacacttcg
     6421 ccgcggcgtt ttttcatcat ttcagccagc ttcgcccgta acgtgcggtt agcactcacg
     6481 ccgcccgcca tgaccagtcg cttaaagccc gtctgatcca acgctcgctt acacttaatc
     6541 atcaacgtat cgaccaccgc atcttcaaag gcgcgggcga tatcagcacg cgtctggtcg
     6601 tcggtgccgt tgtcacgaat cgtgttcgcc gcaaaggttt tcagaccaga aaagctgaaa
     6661 tccagccccg gacggtcggt catcggacgc gggaaaacaa agcgcccggc agtaccctga
     6721 gccgccattt tcgacagtaa cggtccgcca ggataatcca gccccagcag cttcgcggtt
     6781 ttatcaaacg cttcaccggc ggcatcatcg atagactcgc cgagcagctc gtactgacca
     6841 atgccagtca cgctgattaa ctgcgtatga ccgccggaca ccagcagcgc gacaaacgga
     6901 aattccggcg ggttatcttc cagcatcggc gctaacagat gcccttccat atggtgtacc
     6961 gggattgccg gaacgttcca ggcaaacgcc agagaacgcc ccacggttgc gccaaccagc
     7021 agtgcgccga ctaatccagg gcctgcggta taggccacag catcaatgtc ttttgccgtt
     7081 aagccagact cctttagcgc cgcctggatc aacggtacgg ttttgcgcac gtgatcgcgg
     7141 gaggccagtt caggcacgac gccgccatag tcagcgtgca atttcacctg actatacaat
     7201 tggttggcta acaaaccttt ttcatcgtcg taaatggcga tgccggtttc atcgcaggaa
     7261 gtttcaatac ccagtacacg catgacttgt tttacctcgc tttattaccg cgcagtgtag
     7321 gaccaatgcg ggttgatgta aaactttgtt cgcccctgga gaaagcctcg tgtatactcc
     7381 tcacccttat aaaagtccct ttcaaaaagg ccgcggtgct ttacaaagca gcagcaattg
     7441 cagtaaaatt ccgcaccatt ttgaaataag ctggcgttga tgccagcggc aaaccgaatt
     7501 aatcaaaggt gagaggcaca tgccggtaat taaagtacgt gaaaacgagc cgttcgacgt
     7561 agctctgcgt cgcttcaagc gttcctgcga aaaagcaggt gttctggcgg aagttcgtcg
     7621 tcgtgagttc tatgaaaaac cgactaccga acgtaagcgc gctaaagctt ctgcagtgaa
     7681 acgtcacgcg aagaaactgg ctcgcgaaaa cgcacgccgc actcgtctgt actaattccc
     7741 cgagagcgtt gctctccgat cagaccgagt tgtagttgta aggccgtgct tccgaaagga
     7801 atgcgcggct tattttcgtt tatgaattgc taaaaatcgg ggcctatggc tggacgaatc
     7861 ccacgcgtat tcattaatga tctgctggca cgcactgaca tcgtcgatct gatcgatgcc
     7921 cgtgtgaagc tgaaaaagca gggcaagaat ttccacgcgt gttgtccatt ccacaacgag
     7981 aaaaccccgt cattcaccgt taacggtgag aaacagtttt accactgctt tggatgtggc
     8041 gcgcacggca acgcgatcga cttcctgatg aactacgaca agctcgagtt cgttgaaacg
     8101 gtcgaagagc tggcagcaat gcacaatctt gaagtgccat ttgaagcagg cagcggcccc
     8161 agccagatcg agcgccatca gaggcaaacg ctttatcagt tgatggacgg tctgaatacg
     8221 ttttaccaac aatctttaca acaacctgtt gccacgtctg cgcgccagta tctggaaaaa
     8281 cgcggattaa gccacgaggt tatcgctcgc tttgcgattg gttttgcgcc ccccggctgg
     8341 gacaacgtcc tgaagcggtt tggcggcaat ccagaaaatc gccagtcatt gattgatgcg
     8401 gggatgttgg tcactaacga tcagggacgc agttacgatc gtttccgcga gcgggtgatg
     8461 ttccccattc gcgataaacg cggtcgggtg attggttttg gcgggcgcgt gctgggcaac
     8521 gataccccca aatacctgaa ctcgccggaa acagacattt tccataaagg ccgccagctt
     8581 tacggtcttt atgaagcgca gcaggataac gctgaaccca atcgtctgct tgtggtcgaa
     8641 ggctatatgg acgtggtggc gctggcgcaa tacggcatta attacgccgt tgcgtcgtta
     8701 ggtacgtcaa ccaccgccga tcacatacaa ctgttgttcc gcgcgaccaa caatgtcatt
     8761 tgctgttatg acggcgaccg tgcaggccgc gatgccgcat ggcgagcgct ggaaacggcg
     8821 ctgccttaca tgacagacgg ccgtcagcta cgctttatgt ttttgccgga tggcgaagac
     8881 cctgacacgc tggtacgaaa agaaggtaaa gaagcgtttg aagcgcggat ggagcaggcg
     8941 atgccgcttt ccgcatttct gtttaacagc ctgatgccgc aagttgatct gagtacccct
     9001 gacgggcgcg cacgtttgag tacgctggca ctgccattga tatcgcaagt gccgggcgaa
     9061 acgctgcgaa tatatcttcg tcaggaatta ggcaacaaat taggcatact tgatgacagc
     9121 cagcttgaac gattaatgcc aaaagcggca gagagcggcg tttctcgccc tgttccgcag
     9181 ctaaaacgca cgaccatgcg tatacttata gggttgctgg tgcaaaatcc agaattagcg
     9241 acgttggtcc cgccgcttga gaatctggat gaaaataagc tccctggact tggcttattc
     9301 agagaactgg tcaacacttg tctctcccag ccaggtctga ccaccgggca acttttagag
     9361 cactatcgtg gtacaaataa tgctgccacc cttgaaaaac tgtcgatgtg ggacgatata
     9421 gcagataaga atattgctga gcaaaccttc accgactcac tcaaccatat gtttgattcg
     9481 ctgcttgaac tgcgccagga agagttaatc gctcgtgagc gcacgcatgg tttaagcaac
     9541 gaagaacgcc tggagctctg gacattaaac caggagctgg cgaaaaagtg atttaacggc
     9601 ttaagtgccg aagagcgatc gggaagcccc cgacagccgc actgagaggc agcggcaaat
     9661 atataagtac gccctcgtaa ttatcgttgg cggtaaacaa ccgttggatt tcagcgttaa
     9721 cggctgaagg acatcgggtc aatcgcccaa caccaacctc atgaaataag tgtggatacc
     9781 gtcttatgga gcaaaacccg cagtcacagc tgaaacttct tgtcacccgt ggtaaggagc
     9841 aaggctatct gacctatgcc gaggtcaatg accatctgcc ggaagatatc gtcgattcag
     9901 atcagatcga agacatcatc caaatgatca acgacatggg cattcaggtg atggaagaag
     9961 caccggatgc cgatgatctg atgctggctg aaaacaccgc ggacgaagat gctgccgaag
    10021 ccgccgcgca ggtgctttcc agcgtggaat ctgaaatcgg gcgcacgact gacccggtac
    10081 gcatgtacat gcgtgaaatg ggcaccgttg aactgttgac ccgcgaaggc gaaattgaca
    10141 tcgctaagcg tattgaagac gggatcaacc aggttcaatg ctccgttgct gaatatccgg
    10201 aagcgatcac ctatctgctg gaacagtacg atcgtgttga agcagaagaa gcgcgtctgt
    10261 ccgatctgat caccggcttt gttgacccga acgcagaaga agatctggca cctaccgcca
    10321 ctcacgtcgg ttctgagctt tcccaggaag atctggacga tgacgaagat gaagacgaag
    10381 aagatggcga tgacgacagc gccgatgatg acaacagcat cgacccggaa ctggctcgcg
    10441 aaaaatttgc ggaactacgc gctcagtacg ttgtaacgcg tgacaccatc aaagcgaaag
    10501 gtcgcagtca cgctgccgct caggaagaga tcctgaaact gtctgaagta ttcaaacagt
    10561 tccgcctggt gccgaagcag tttgactacc tggtcaacag catgcgcgtc atgatggacc
    10621 gcgttcgtac gcaagaacgt ctgatcatga agctctgcgt tgagcagtgc aaaatgccga
    10681 agaaaaactt cattaccctg tttaccggca acgaaaccag cgatacctgg ttcaacgcgg
    10741 caattgcgat gaacaagccg tggtcggaaa aactgcacga tgtctctgaa gaagtgcatc
    10801 gcgccctgca gaaactgcag cagattgaag aagaaaccgg cctgaccatc gagcaggtta
    10861 aagatatcaa ccgtcgtatg tccatcggtg aagcgaaagc ccgccgtgcg aagaaagaga
    10921 tggttgaagc gaacttacgt ctggttattt ctatcgccaa gaaatacacc aaccgtggct
    10981 tgcagttcct tgacctgatt caggaaggca acatcggtct gatgaaagcg gttgataaat
    11041 tcgaataccg ccgtggttac aagttctcca cctacgcaac ctggtggatc cgtcaggcga
    11101 tcacccgttc tatcgcggat caggcgcgca ccatccgtat tccggtgcat atgattgaga
    11161 ctatcaacaa actcaaccgt atttctcgcc agatgctgca agagatgggc cgtgaaccga
    11221 cgccggaaga actggctgaa cgtatgctga tgccggaaga caagatccgc aaagtgctga
    11281 agatcgccaa agagccaatc tccatggaaa cgccgatcgg tgatgatgaa gattcgcatc
    11341 tgggggattt catcgaggat accaccctcg agctgccgct ggattctgcg accaccgaaa
    11401 gcctgcgtgc ggcaacgcac gacgtgctgg ctggcctgac cgcgcgtgaa gcgaaagttc
    11461 tgcgtatgcg tttcggtatc gatatgaaca ccgaccacac gctggaagaa gtgggtaaac
    11521 agttcgacgt tacccgcgaa cgtatccgtc agatcgaagc gaaggcgctg cgcaaactgc
    11581 gtcacccgag ccgttctgaa gtgttgcgta gcttcctgga cgattaatcg gtaggccgga
    11641 tcaggcgtta cgccgcaccc ggcactagtc cctctgcaca aacgccacct ttt
//
LOCUS       DAZPAC010000125        11355 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZPAC010000125 DAZPAC010000000
VERSION     DAZPAC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.125"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..535)
                     /gene="espL1"
                     /locus_tag="ACHUPM_002818"
     CDS             complement(<1..535)
                     /gene="espL1"
                     /locus_tag="ACHUPM_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFR0870545.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQY"
     gene            1060..2988
                     /gene="thrS"
                     /locus_tag="ACHUPM_002819"
     CDS             1060..2988
                     /gene="thrS"
                     /locus_tag="ACHUPM_002819"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR0870546.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2992..3534
                     /gene="infC"
                     /locus_tag="ACHUPM_002820"
     CDS             2992..3534
                     /gene="infC"
                     /locus_tag="ACHUPM_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR0870547.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3631..3828
                     /gene="rpmI"
                     /locus_tag="ACHUPM_002821"
     CDS             3631..3828
                     /gene="rpmI"
                     /locus_tag="ACHUPM_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR0870548.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3881..4237
                     /gene="rplT"
                     /locus_tag="ACHUPM_002822"
     CDS             3881..4237
                     /gene="rplT"
                     /locus_tag="ACHUPM_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR0870549.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4354..4478
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4360..4404
                     /gene="pheM"
                     /locus_tag="ACHUPM_002823"
     CDS             4360..4404
                     /gene="pheM"
                     /locus_tag="ACHUPM_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR0870550.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4687..5670
                     /gene="pheS"
                     /locus_tag="ACHUPM_002824"
     CDS             4687..5670
                     /gene="pheS"
                     /locus_tag="ACHUPM_002824"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR0870551.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5685..8072
                     /gene="pheT"
                     /locus_tag="ACHUPM_002825"
     CDS             5685..8072
                     /gene="pheT"
                     /locus_tag="ACHUPM_002825"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR0870552.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8077..8376
                     /gene="ihfA"
                     /locus_tag="ACHUPM_002826"
     CDS             8077..8376
                     /gene="ihfA"
                     /locus_tag="ACHUPM_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR0870553.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8477..9457
                     /gene="btuC"
                     /locus_tag="ACHUPM_002827"
     CDS             8477..9457
                     /gene="btuC"
                     /locus_tag="ACHUPM_002827"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR0870554.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9520..10071
                     /gene="btuE"
                     /locus_tag="ACHUPM_002828"
     CDS             9520..10071
                     /gene="btuE"
                     /locus_tag="ACHUPM_002828"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR0870555.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10071..10820
                     /gene="btuD"
                     /locus_tag="ACHUPM_002829"
     CDS             10071..10820
                     /gene="btuD"
                     /locus_tag="ACHUPM_002829"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR0870556.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10898..>11355
                     /locus_tag="ACHUPM_002830"
     CDS             10898..>11355
                     /locus_tag="ACHUPM_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR0870557.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2873 a         2631 c         3055 g         2796 t
ORIGIN      
        1 cgtattgcaa tgacagatgg cgacaaaaaa tagcgtcaga aggagattgc gaaaaaaatg
       61 cactaccatt gagatttact gttggttgta tagttttttc tctatggatt tttttagaca
      121 gtttcataca atggttacga tcttcggcag ttaactgttc ctgttcaata taatccagta
      181 tgcttcgcaa atggttagcc ttaataggcg tggtttccag aaataaggga atgatttctt
      241 ttttatagtg taatgtaatg agtatatttt ctgcatggat gggtgaagtg gcaaagttaa
      301 tgataaaaag ggcagtggaa taacaatttt gtgaaatttt tcgacagttt gcggtaaatt
      361 cctgagtaac acagtatgac agcatcgaac ttaagtcaga ggtatcaagt agggtgatga
      421 gttggcgcgc aaattgatta ataggctctg atgaaggcgt tggtatgtcg ctatcgatgt
      481 gcaatctatg aggagactta taatgggttc tgataatgat atcgttttga ctcataatct
      541 attcgcctta tatatgtctg gaggttttga ggatgatgat taattattat cgccagaacg
      601 aaatgtgcac gaaaaattgc tataagaatt gtgtcacttt tatcattgtg atatgaccac
      661 ttcgtcatta atctcatgag cgtttaaaat actaccgcca aatcacgcaa cgaactttgc
      721 tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa cctttcagac gcaccgtgat
      781 gttatcagtg gttctttaag cgttttgctg gtgtactcac tacaaacgaa ttgcgaatcg
      841 aatcaatgtg aaacggaaag gtacaatctc ccttttttta gtgttaacat cgttcaaccg
      901 ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct gattgcgaac caatttagca
      961 tttgttggct agatggtttc gcaatgaact gttaataaac aaatttttct ttgtatgtga
     1021 tctttcgtgt gggtcaccac tgcaaattaa ggatataaaa tgcctgttat aactcttcct
     1081 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
     1141 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
     1201 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
     1261 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
     1321 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
     1381 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
     1441 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1501 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1561 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1621 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1681 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1741 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1801 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1861 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1921 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1981 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     2041 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     2101 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     2161 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     2221 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     2281 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     2341 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     2401 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     2461 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2521 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2581 gtaatgattc accgcgcaat tctggggtcg atggaacgtt tcatcggtat cctgaccgaa
     2641 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2701 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2761 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2821 cgtcgcgtcc catatatgtt ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2881 gttcgcaccc gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag
     2941 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     3001 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     3061 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     3121 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     3181 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     3241 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tggtacagat
     3301 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     3361 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     3421 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tggtcgaatc cttcccaacg
     3481 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3541 caagtagcac tgtctgtgga gccttcgggc ttcacaggct ttgttcgcct ttgtttcgtt
     3601 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3661 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3721 cacattctga ccaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3781 tccaaaggcg atctgggcct ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3841 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3901 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3961 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     4021 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     4081 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     4141 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     4201 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     4261 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     4321 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     4381 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     4441 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4501 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagcg cggagaaacc
     4561 ggagcgtatt taagtacgtg agaatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4621 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4681 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4741 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4801 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4861 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4921 gaaagcgcag cgctgaatgc gcgtctggcg gcggaaacga ttgatgtctc cctgctaggt
     4981 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     5041 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     5101 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     5161 tggtttgaca ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     5221 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gcgtttatcg taacgactac
     5281 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     5341 agctttacca acctgaaagg cactctgcac gatttcctgc gtaacttctt tgaggaagat
     5401 ttgcagattc gcttccgtcc ttcctacttc ccgttcaccg agccttctgc agaagttgac
     5461 gtcatgggta aaaacggtaa atggctggaa gtgctgggtt gcgggatggt gcatccgaac
     5521 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5581 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5641 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5701 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tgcgctggcg aatcaaatca
     5761 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5821 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5881 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg tggtgcgcca aactgccgtc
     5941 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     6001 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttc tctgaactgg
     6061 gaatttctga cgatcacagc ggcattatcg aactgcctgc agatgcgccg attggcactg
     6121 acatccgcga atacctgaaa ctcgatgaca acaccatcga aatcagcgta acgccaaacc
     6181 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     6241 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     6301 cagtcgaagc gcctgaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     6361 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     6421 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6481 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa
     6541 cgctggtact gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6601 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6661 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6721 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6781 cggcactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6841 gtgaggctgg tccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6901 tcactttacg tcgtagcaaa ctggatcgcc tgatcggcca tcatattgct gatgaacagg
     6961 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gagtggcagg
     7021 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtt gaagaagtcg
     7081 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     7141 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     7201 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     7261 caggcgttga agccttactg ctgccaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     7321 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     7381 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     7441 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7501 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgatcttgaa tccgttctcg
     7561 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7621 cggggcaatc cgcagcgatt tatctgaaag gggaacgtat tggttttgtt ggagttgttc
     7681 atcctgaact ggaacgtaaa ctggatctta acggtcgcac tctggtgttc gaactggagt
     7741 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7801 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7861 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7921 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7981 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     8041 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     8101 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     8161 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     8221 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     8281 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     8341 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     8401 ctgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     8461 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8521 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8581 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8641 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8701 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8761 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8821 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8881 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8941 ctgtagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     9001 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     9061 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     9121 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     9181 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     9241 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     9301 acttcccggc tgcgcgctgg caggggcgag cgcagtgctg ctggccgata ttgtagcgcg
     9361 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     9421 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9481 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9541 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9601 ctgttgattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9661 aatattcaga aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9721 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9781 ggggtgacgt tcccgatgtt cagtaagatt gaagttaacg gcgaaggacg ccatccgctg
     9841 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9901 gcccgtatgg tcagcaaagg tcgtgcaccg ctgtaccctg atgatatttt atggaatttt
     9961 gagaaatttt tagttggcag ggacggattg gtcatccagc gtttttcccc ggatatgacg
    10021 ccggaaaacc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
    10081 tgatgcagtt acaggatgtt gcggaatcta cccgcctagg gccactttct ggcgaggttc
    10141 gggctgggga gatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
    10201 cacgaatggc cggaatgacc agcggtaagg gaagtattca gttcgcgggg caaccactgg
    10261 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
    10321 cgccgtttgc aatgccggtc tggcactatc tgacactgca tcagcacgat aaaacgcgta
    10381 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggacgtagca
    10441 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgctgcaaa
    10501 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10561 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgctctgtgt cagcaaggac
    10621 tggcgattgt gatgagcagt cacgatctca accacacatt gcgtcatgcg catcgggcgt
    10681 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10741 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10801 tgctgatttc gaccatctga tttcattagt atcttgaaaa tcgggtaatt acgacgctaa
    10861 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10921 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10981 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    11041 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    11101 aacgatgcgc gatcgtttcg atttgcagct gccccgagaa accaaagaac aagcctctat
    11161 cggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    11221 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    11281 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    11341 ctggcaggcg agacg
//
LOCUS       DAZPAC010000126        11300 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZPAC010000126 DAZPAC010000000
VERSION     DAZPAC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11300
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.126"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            75..209
                     /locus_tag="ACHUPM_002831"
     CDS             75..209
                     /locus_tag="ACHUPM_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR0870558.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            188..292
                     /gene="speFL"
                     /locus_tag="ACHUPM_002832"
     CDS             188..292
                     /gene="speFL"
                     /locus_tag="ACHUPM_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR0870559.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            603..2801
                     /gene="speF"
                     /locus_tag="ACHUPM_002833"
     CDS             603..2801
                     /gene="speF"
                     /locus_tag="ACHUPM_002833"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFR0870560.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2798..4117
                     /gene="potE"
                     /locus_tag="ACHUPM_002834"
     CDS             2798..4117
                     /gene="potE"
                     /locus_tag="ACHUPM_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR0870561.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4173..5813)
                     /gene="pgm"
                     /locus_tag="ACHUPM_002835"
     CDS             complement(4173..5813)
                     /gene="pgm"
                     /locus_tag="ACHUPM_002835"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR0870562.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5839..6384)
                     /gene="seqA"
                     /locus_tag="ACHUPM_002836"
     CDS             complement(5839..6384)
                     /gene="seqA"
                     /locus_tag="ACHUPM_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR0870563.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6569..7333
                     /gene="ybfF"
                     /locus_tag="ACHUPM_002837"
     CDS             6569..7333
                     /gene="ybfF"
                     /locus_tag="ACHUPM_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0870564.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7473..7766
                     /gene="ybfE"
                     /locus_tag="ACHUPM_002838"
     CDS             7473..7766
                     /gene="ybfE"
                     /locus_tag="ACHUPM_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR0870565.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7906..8436
                     /gene="fldA"
                     /locus_tag="ACHUPM_002839"
     CDS             7906..8436
                     /gene="fldA"
                     /locus_tag="ACHUPM_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR0870566.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8646..8732
                     /gene="uof"
                     /locus_tag="ACHUPM_002840"
     CDS             8646..8732
                     /gene="uof"
                     /locus_tag="ACHUPM_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR0870567.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8725..9171
                     /gene="fur"
                     /locus_tag="ACHUPM_002841"
     CDS             8725..9171
                     /gene="fur"
                     /locus_tag="ACHUPM_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR0870568.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9256..9582)
                     /gene="chiQ"
                     /locus_tag="ACHUPM_002842"
     CDS             complement(9256..9582)
                     /gene="chiQ"
                     /locus_tag="ACHUPM_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR0870569.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9632..11038)
                     /gene="chiP"
                     /locus_tag="ACHUPM_002843"
                     /pseudo
     CDS             complement(9632..11038)
                     /gene="chiP"
                     /locus_tag="ACHUPM_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2767 a         2779 c         2812 g         2942 t
ORIGIN      
        1 aacgcaaata taaaacacga aattacaaca tttaagtaac aaaacagcat ctaatatggc
       61 gatattcata attaatgatt tattatctgg ggtatgatcg ttttttgttg atcttcttca
      121 cagattatag ccatttcatg gatagaataa ctctaccttc aactgacaca gcaagaggta
      181 aaggtaaatg gaaaataata accgcttaat gcctcatata aggcggacaa cccatatcat
      241 gaagtttgcc catcgtaata gttttgactt tcatttcttt aatgcccgct agtcttctga
      301 ctaaagggca ccccaacgta caggtctccc tgactttaag cattacaggt taatacctgt
      361 attcctcggt gctcatatac tgctaaccct tttaaactct aaataattcg agtcgcagca
      421 cttgcaactt gaggtatgac gagtatagcc agttaccggg ctggtctggg ttattgcatc
      481 tgcaaaaagc aaactactga tttatttatc agcggtggag ctttgctttt tttccggcgt
      541 gatcgatttc tcctttgaga aattgaggac ctgctattac ctgaaataaa gagatgaata
      601 aaatgtcaga attaaaaatt gccgttagcc gttcttgccc ggattgtttt tccactcatc
      661 gtgcatgcgt gaatattgac gaaagtaatt atattgacgt tgccgccatt attttatcag
      721 tcaatgatgt tgaacgtgga aaactcgatg aaatagacgc tactggctat ggcattcctg
      781 tttttattgc aacggaaaat gaagaacgtg taccagcaga atatctgcca cgtatttctg
      841 gtgtcttcga gcattgcgaa tcacggaaag agttttatgg acgtcagtta gaaaccgctg
      901 ctagccatta tgaaacgcaa ctgcgcccgc ccttcttccg cgcactggtc gattatgtca
      961 atcagggcaa tagcgctttt gactgcccgg gtcatcaggg aggtgagttt ttccgtcgtc
     1021 atccggcagg gaatcagttc gtggagtact tcggagaaac gctgttccgt tccgacttat
     1081 gcaacgcaga cgtagcaatg ggcgatctgc tgattcacga aggcgcgcca tgcattgcac
     1141 agcaacatgc ggcaaaagtg tttaatgccg ataaaacata cttcgttcta aatggcactt
     1201 catcttctaa caaagtggtt ttaaacgccc tgctaacacc gggtgatctg gtgctgtttg
     1261 atcgcaataa ccacaaatct aaccaccacg gagcgttgct acaggctggt gcaacaccgg
     1321 tttatctgga aacggcacgt aacccgtatg gctttatcgg tggcattgat gcgcactgtt
     1381 ttgaagaaag ttacctgcgt gagctgatcg cggaagtcgc accgcagcgg gcaaaagagg
     1441 ctcgtccttt ccgcctcgct gtgattcagt taggcaccta cgacggtacg atttataacg
     1501 cccgccaggt ggtggataaa attggtcatc tgtgtgacta cattctgttt gactccgcat
     1561 gggttggcta tgagcaattt atcccgatga tggctgactg ttcgccgctg ttgctggatc
     1621 ttaatgagaa cgatccgggt attctggtta cgcaatccgt acataaacag caggctggtt
     1681 tttctcaaac ttcacaaatc cataaaaaag acagccacat caaagggcaa cagcgttatg
     1741 taccgcacaa acgcatgaac aacgccttta tgatgcacgc ctccaccagc ccgttctatc
     1801 cgctgtttgc cgcactggat atcaacgcca aaatgcatga aggtgtcagc ggtcgtaata
     1861 tgtggatgga ttgtgtggta aatggcatta atgcccgcaa actgatcctc gataactgtc
     1921 agcatattcg tccgttcgta cctgaactgg tggatggtaa accctggcag tcgtatgaaa
     1981 cagcgcaaat tgcggttgat ctgcgcttct tccagtttgt accaggggaa cactggcatt
     2041 cttttgaagg ctatgcagag aatcaatact ttgtcgatcc ttgcaaactg ttgctgacaa
     2101 ccccaggtat tgatgcacgt aacggcgaat atgaagcgtt cggtgtaccc gcgacgattc
     2161 ttgctaactt cctgcgcgaa aatggcgtag tgccggaaaa atgcgatctt aactccatcc
     2221 tcttcctgct gactccggca gaagatatgg ccaaacttca gcaacttgtt gccctgctgg
     2281 tacgcttcga aaaactgctt gagtccgacg cgccattagc agaagtgctg ccttccatct
     2341 acaaacagca tgaagagcgc tacgccggtt ataccctgcg tcagttgtgc caggaaatgc
     2401 atgatttgta tgcccgccac aacgtgaaac aactgcaaaa agagatgttc cgcaaagcgc
     2461 acttcccaca cgtcagcatg aatccgcaag aagccaacta cgcctattta cgcggtgaag
     2521 tggagctggt tcgtctgccg gatgccgaag gtcgtatcgc tgccgaaggg gcgcttcctt
     2581 atcctccggg tgtgctgtgt gttgttccgg gtgaaatctg gggtggtgct gttttgcgtt
     2641 acttcagcgc tctggaagaa gggatcaacc tgctgccagg ttttgcaccg gagctgcagg
     2701 gtgtctatat cgaagaacat gatggtcgta agcaagtttg gtgctatgtc atcaagcctc
     2761 gtgatgcgca aagcaccctg ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa
     2821 aatgggcgtc gttcagttaa ccatactgac gatggtcaac atgatgggct ccggtatcat
     2881 catgctgccg acaaagcttg ccgaagttgg gacaatttca attatctcct ggctggtgac
     2941 agccgtaggc tcaatggcac tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa
     3001 atcaggtggt atgggcggtt atgccgagta tgctttcggt aaatccggta actttatggc
     3061 gaactatacc tacggcgtct cactgctgat tgctaacgtc gcgattgcta tttcggcggt
     3121 tggttacggc accgaactgc tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac
     3181 catcggggtg ctgtggattt gtaccgtggc taactttggt ggtgcgcgca ttaccgggca
     3241 aatcagtagc attaccgtgt ggggggtcat tattccggtg gtaggcctgt gcattatcgg
     3301 ctggttctgg tttagcccga cgctttacgt tgattcctgg aatccgcatc atgcaccgtt
     3361 cttcagtgca gtaggttctt ccatcgctat gacgctgtgg gcttttctcg gtctggagtc
     3421 tgcgtgtgcg aatactgatg tagtggaaaa cccggaacgt aatgtgccaa tcgcggtact
     3481 cggcggtacg ttaggtgcgg cggtgattta tatcgtctcc accaacgtga ttgcagggat
     3541 tgtgcctaat atggagctgg caaattcaac ggcaccattt ggcctggcct tcgcacagat
     3601 gttcacacca gaagtgggta aagtcattat ggcgctgatg gtgatgtcct gctgcggttc
     3661 gctgcttggc tggcagttca ccattgccca ggtgtttaag tcttcatctg atgaaggcta
     3721 cttccctaaa attttctccc gtgtaaccaa agtggatgca ccggtgcagg gaatgttgac
     3781 cattgtgatt atccagagcg ggttggcgct gatgacgatt agcccgtcgc taaacagtca
     3841 gttcaacgtg ctggttaacc tggccgtggt gaccaatatc attccgtata ttctgtcaat
     3901 ggcggcatta gtcattattc agaaggtcgc taatgtgccc ccctcaaaag cgaaagttgc
     3961 aaactttgtt gcttttgttg gcgcgatgta tagcttttat gcgctgtact catccgggga
     4021 agaagccatg ctgtacggtt ccatcgtgac cttccttggt tggacactgt atggtctggt
     4081 atcaccacgc tttgaactga aaaataaaca cggttaaaaa agcaaagtaa aaaagggcga
     4141 tcttgcgacc gccctttttt tattaaatgt gtttacgcgt ttttcagaac ttcgctaaca
     4201 atctcaaccg cttctttctc aatctgcttg cgatgttctt caccgaggaa gctttcgcag
     4261 tagatcttat atgcgtcttc cgtgcctgac ggacgcgcgg cgaaccagcc gttgtcagtc
     4321 atcactttca gaccgccgat agaagcacca ttgcccggtg ccgccgtcag gcgcgcggtg
     4381 atcgggtcgc ctgccagggt gctggcgctc accatttccg gagacagctt agacagcgcc
     4441 gctttttgtg cggaagtcgc agctgcctgc aaacggttgt agctcggcgc accaaagcgt
     4501 tttgccagtt cgttgtagtg ttcctgcggg ttcttaccgg tgacagcggt gatttccgcc
     4561 gccagcagac acatgatgat gccgtctttg tcggtggacc acggcgtgcc gtcgaaacgc
     4621 aggaaggaag cccctgcgct ctcttcgccg ccaaagccga agctgccgtc gaacagacca
     4681 tcaacaaacc atttgaaacc taccgggact tctaccagct tgcggcccaa gtcattgacc
     4741 acacggtcga tcatcgcaga agaaaccagc gttttaccga cagcaacatc tttgccccac
     4801 tgcggacgat gctggaacag gtagttgatc gccaccgcca ggtagtggtt cggattcatc
     4861 aaacctgccg gagtgacgat accgtgacgg tcataatccg ggtcgttagc aaacgccaga
     4921 tcgaacttat cacgcaatgc cagcaggccc gccatcgcac actcggagga gcagtccata
     4981 cggatcgcgc cgtctttatc gaggtgcata aagcggaagg tttgatcgac ctgatcgtta
     5041 acgatggtca ggttgaggtt gtaatactcg ccaattcgct tccagtattc gataccggaa
     5101 ccacccagcg gatcaacgcc cagcgtcagg cccgttttct gaatcgccgc catatcaacg
     5161 atatcggcca gaccttccac aaactgctgc accagatcct gctctttcac atgaccggat
     5221 gccatcgctt cgtcgaggga gatacgcttc acgcctttca ggccatcggc catcagtgcg
     5281 ttggcacgat cttccaccac tttagtgacg ttggtatcag ccgggccacc atttggcgga
     5341 ttgtacttga taccaccatc ttccggcggg ttatgggagg gtgtaatcac gataccgtct
     5401 gccagcgggc cacctttttt attatgaacc aggatggcat tggaaacggc aggcgtcgga
     5461 gtgaagccat tgttttcctg cacaatgaca tcaacgccgt tcgctgccag cacttccagt
     5521 acggaaatga acgcaggctc ggacagggcg tgagtatctt tacccacata gcaagggcca
     5581 gtgatgccgt ttttcgcacg ttcttcagca attgcctgag cgatcgccag aatgtgcggc
     5641 tcgttaaagc tgtggcgcgc tgcactgcca cggtgaccgg aagtaccgaa tttcaccgcg
     5701 tgctccgcat tccctgcttc tggtttcagt acataatatt gcgccgtcag ttgggcgacg
     5761 ttaatcaaat cactctgttg tgcaggttgg cctgcacgat tgtggattgc cattgctttg
     5821 tcctttgtct gcaacgtttt agatagttcc gcaaaccttc tcaatcaatt ccgccgggaa
     5881 ttgcatcgac tgcatgatgt gttcgatcat gctgcattta cggccggtgt tggtgttggt
     5941 gatcacccaa tacggcgtgc ctggcacatg tttcggcttg gtctgattac catttttcag
     6001 cagcgtttgt tcatctgccg caaagtaaac gcgtgtacga ccgtgcaacg attccgttgc
     6061 ttcggcaaac gcctgggcgt caagagaata tagtgtagac aacagcagca taaagcgatt
     6121 gaccgctcgc ttttgctctg cgtattcgtc agaaagcaga agttcacgca ttgcgcgaac
     6181 cttgtcttta atcgttttga ccggcttcgc ttcgacgata gcaggtgacg caacgcgaac
     6241 ctctttcgtc accggagcag caggctgtga tgcggcggaa aatttcaaca tacgccgtaa
     6301 aatgtcggat gcgctctcgc cgatatgctt agtgtggctg gcaatatagc tgtagagttc
     6361 atcatcaact tcaatcgttt tcatcttaat ccagtacagt gtcttagctg aatacaagtc
     6421 gccaagagta tatgaataaa tcccggcagc ggatagcgtc aagcctggct tgcagaaaaa
     6481 ctcggaataa accttatttg ttgcagccct gtggcacaat ggtcaacata ataccctaac
     6541 ccgacaacgc gaaaaaaaga actttgccat gaaattgaat atccgcgcgc aaactgcaca
     6601 aaaccagcac aataattctc ccatcgttct tgtccatggt ctgtttggta gcctcgacaa
     6661 ccttggcgta ctggctcgcg atctggtaaa cgatcacaat atcatccagg ttgatatgcg
     6721 taaccacggt ctttcaccga gagatccggt aatgaattac ccggcgatgg cgcaggatct
     6781 tgttgatact ctggatgcac agcagatcga caaagcaaca tttatcggtc actccatggg
     6841 cggtaaagcg gtaatggcac ttacggcact ggcccgcgat cgcatcgata aactggtggc
     6901 gatcgatatc gcaccggtcg actatcacgt acgccgtcat gatgagattt tcgcggctat
     6961 caacgcggtc agtgaatcgg acgcacaaac tcgccagcaa gcagcagcaa taatgcacca
     7021 gcatcttaat gaagaagggg tgattcagtt tctgttgaaa tcttttgttg acggggagtg
     7081 gcgctttaac gtgccggtat tgtgggatca gtatccgcat attgtgggtt gggagaaaat
     7141 cccggcatgg gatcaccctg ccctgtttat ccctggcggc aattctccgt atgttagcga
     7201 gcagtaccgt gatgatttac tggcacaatt tccacaggca cgggcgcatg tgattgcagg
     7261 cgcgggtcac tgggtccatg ctgaaaaacc ggatgcggta ttacgcgcta tccgtcgcta
     7321 tctcaatgat taacgcaact gattaaaaac ccagcgtccg cgtcgctttc cgcgcgtggg
     7381 cgttggcgtg actcatcgcg ctgatgtatt atggcgcgct atccatccgg gcagaggcct
     7441 ggctgattgt ttcccccgaa gtcaccaaga tcatggccaa agaacaaacg gaccgtacga
     7501 cattagatct gttcgcgcac gagcgtcgac cgggacgacc gaaaactaat ccgctttcgc
     7561 gcgatgaaca gctgcgtatt aataaacgca accagctaaa acgcgacaaa gtacgtggcc
     7621 ttaagcgtgt cgaactgaag ctgaacgcgg aagctgtcga ggcgctgaac gagctggcgg
     7681 agtcgcgcaa tatgagccgt agcgaactga tcgaagagat gttaatgcag caactggcgg
     7741 cgctgcgtag tcagggcatc gtttaaattt ccactttcat gtagcacagt gtgcagtcct
     7801 gctcgtttgc tgattccgca agactgcctg ttctgctatg attgccttta tccgtgggca
     7861 attttccacc cccatttcaa taagtttcaa gaggttattt cactcatggc tatcactggc
     7921 atctttttcg gcagcgacac cggtaatacc gaaaatatcg caaaaatgat tcaaaaacag
     7981 cttggtaaag acgttgccga tgtccatgac attgcaaaaa gcagcaaaga agatctggaa
     8041 gcttatgaca ttctgctgct gggcatccca acctggtatt atggcgaagc gcagtgtgac
     8101 tgggatgact tcttcccgac tctcgaagag attgatttca acggcaaact ggttgcgctg
     8161 tttggttgtg gtgaccagga agattacgcc gaatatttct gcgacgcatt gggcaccatc
     8221 cgcgacatca ttgaaccgcg cggtgcaacg atcgttggtc actggccaac tgcgggctat
     8281 catttcgaag catcaaaagg tctggctgat gacgaccact ttgtcggtct ggctatcgac
     8341 gaagaccgtc agccggaact gaccgctgaa cgtgtagaaa aatgggttaa acagatttct
     8401 gaagagttgc atctcgacga aattctcaat gcctgatgtg atgcggcgta gactcatgtc
     8461 tacgccgtat taatagataa tgccaatcaa aataattgct acaaatttgt aacttttgct
     8521 gttgtacctg tacaatgtcc cggtgttcaa gtggccttgc cgttgtaaat gtaagctgtg
     8581 ccacgttttt attaacaata tttgccaggg acttgtggtt ttcatttagg cgtggcaatt
     8641 ctataatgat acgcattatc tcaagagcaa attctgtcac ttcttctaat gaagtgaacc
     8701 gcttagtaac aggacagatt ccgcatgact gataacaata ccgccctaaa gaaagctggc
     8761 ctgaaagtaa cgcttcctcg tttaaaaatc ctagaagttc ttcaggagcc ggacaaccat
     8821 cacgtcagtg cggaagattt atacaaacgt ctgatcgata tgggtgaaga aattggtctg
     8881 gctacggtat atcgcgtact gaaccagttt gacgacgctg gtatcgtcac ccgccacaat
     8941 tttgaaggcg gtaaatccgt atttgaactg acacagcaac atcaccacga tcacctgatc
     9001 tgcctcgact gcggcaaggt tatcgaattt agtgatgatt ccatcgaagc gcgtcagcgt
     9061 gaaattgccg caaaacatgg cattcgcctg actaaccaca gtctctatct ttacggtcac
     9121 tgtgccgaag gcgattgccg cgaagatgaa catgcgcacg aaggcaaata agccagcctg
     9181 aatgagaaaa agccaacctg cgggttggct tttttatgca agggaaataa gaaaagcgag
     9241 agttacagct ctcacttatt tgttattact gcgaatttcc tgccaaactt tatcacaatc
     9301 ggctttcacc gcctgatcat ttccggtttg cgttgcacgc agatactgct gataatccag
     9361 tacgcggaca ctttcctggt cggcaaaatg ctgatgttgt ttctctttct tcagcacgtt
     9421 taacacgctc tggcaggctt caattttttc tggcgaacct tgtgcggtgt tgatacaggc
     9481 gctgtatgcc tctttcagac gactgtcttc ctgtggcgcg gttgattgcg cacaagctac
     9541 cagccccgat gccattatgg cgatgagaat cagttttttc atagcaaatt cctcaaacgt
     9601 gcggcaggca caaaacctgc cgcgtcgggc atcagaagat ggtgaatggt gcgattacca
     9661 taaatttcac gtcacgctca tcctggaaga tgttgccgta accaccgccc cagcttggga
     9721 tgtcggagtg gttgtcgtat tcggtgaagt gcagtttgaa catcgtgcct ttggcgcgac
     9781 cgtcctgaat ggtgtagacc gcatccaggc tgtatgcaga ctcttcaata gtccggtttt
     9841 tgtcgtagta cgcatccgga ttgctctgct aggtcgcagg tttagcatcc catgcgtaaa
     9901 cgtaggaagc gccgatggcg aagcctggaa gattccagtt tttcaggtca tacatcgcac
     9961 cgaagaagac cgctttttcg ccgttagcgt tgaagtcgga acggttatcc caccagatat
    10021 ccaggcggcc gttggaggaa gcgtaggttg gagtcatacg ttgcaggaag tatccctgct
    10081 gaccgtcagc cttaacccag gtgccttcga ggcgcaaatc cactacgtca gccgcccggt
    10141 aaccaaaggt caacgcttgc agccaggcgg tgccgtcata aaggtcgttg acgctgcgat
    10201 cgtcaacttt atcgcgcgta ccatagaact ggtagctggt ggttaacggg ctaccggcga
    10261 tatcaaattt gtagctggct ttggcaaaat attgatcgat atacccttcc gcctgaccaa
    10321 atgccgcttc cagtacgaag ttatttttga agtcgtattt cgccccaagg gagtgcagat
    10381 aatcaacttt ggtggtttta tcgttctgat aaaactcatc catttccaga tgccacggcg
    10441 ctttgtattc gttggtccac atgtaggaga aactcaacgc accagcatcg ccgtaatcaa
    10501 aattcgcccc ggcttccgcc ccctgataag tacctggcat aaagctccag tgcggcgcta
    10561 acagcgtttg accagttggc tgaatgtaac ctgccctcgc ccaaaccgga ccgtatttaa
    10621 atttggccgc agctttatac aggcttatac cgcttttgtc gccggaccag tcttcgtcat
    10681 aggctttatt actttttgaa aacgcgattt cgttcgggtg ggagctgtcg ccgttttccg
    10741 ccatttcaat cgccgtaaac gcagcaatat caaggccgaa catatcagca gcataaccgg
    10801 actgaaaatc gaggttggca ttccaggtgg agtgagaaag gttggttttg tatttgtcgc
    10861 cgtcggtaac atctttacgg tcgcgttcac gctgccagta atatataccg ccggttaagg
    10921 ttgaatcgtc gatgaatcct tcggcgcgag cctccggcat tgccataaag cccgacattg
    10981 ctgtaacacc ggcgatagcc agcgccagcg tactacgttt gccactaaac gtacgcatgg
    11041 gttaatcctc tttgacgtat aaattgctgc accaaaggtg aacagcgaaa aaataaaaat
    11101 taaaaaagta gcgttgagaa aagtctcgct gactacagga acagactatt tttcgcgacg
    11161 cgaattatca atctctttct gtaaccagaa tgtaagaaca tgagcaaccg cacatatttt
    11221 gatgattttt gttacaaggc ttaagtttgc ggttgaaagg cttgcagaag aataaaagcg
    11281 tgaaattcca gcaagatgaa
//
LOCUS       DAZPAC010000127        11287 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZPAC010000127 DAZPAC010000000
VERSION     DAZPAC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11287
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.127"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHUPM_002844"
     CDS             <1..122
                     /locus_tag="ACHUPM_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFR0870570.1"
                     /translation="YATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM"
     gene            complement(321..671)
                     /locus_tag="ACHUPM_002845"
                     /pseudo
     CDS             complement(321..671)
                     /locus_tag="ACHUPM_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(874..1887)
                     /gene="intI1"
                     /locus_tag="ACHUPM_002846"
     CDS             complement(874..1887)
                     /gene="intI1"
                     /locus_tag="ACHUPM_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR0870571.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2045..2518
                     /gene="dfrA17"
                     /locus_tag="ACHUPM_002847"
     CDS             2045..2518
                     /gene="dfrA17"
                     /locus_tag="ACHUPM_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR0870572.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            2649..3437
                     /locus_tag="ACHUPM_002848"
     CDS             2649..3437
                     /locus_tag="ACHUPM_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFR0870573.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            3643..3990
                     /locus_tag="ACHUPM_002849"
     CDS             3643..3990
                     /locus_tag="ACHUPM_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR0870574.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            3984..4823
                     /gene="sul1"
                     /locus_tag="ACHUPM_002850"
     CDS             3984..4823
                     /gene="sul1"
                     /locus_tag="ACHUPM_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR0870575.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            4951..5154
                     /locus_tag="ACHUPM_002851"
     CDS             4951..5154
                     /locus_tag="ACHUPM_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870576.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            5310..6515
                     /gene="chrA"
                     /locus_tag="ACHUPM_002852"
     CDS             5310..6515
                     /gene="chrA"
                     /locus_tag="ACHUPM_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFR0870577.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            6526..6831
                     /locus_tag="ACHUPM_002853"
     CDS             6526..6831
                     /locus_tag="ACHUPM_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR0870578.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            7058..7822
                     /locus_tag="ACHUPM_002854"
     CDS             7058..7822
                     /locus_tag="ACHUPM_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFR0870579.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(8315..8899)
                     /gene="mphR(A)"
                     /locus_tag="ACHUPM_002855"
     CDS             complement(8315..8899)
                     /gene="mphR(A)"
                     /locus_tag="ACHUPM_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFR0870580.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(8899..10137)
                     /gene="mrx(A)"
                     /locus_tag="ACHUPM_002856"
                     /gene_synonym="mrx"
     CDS             complement(8899..10137)
                     /gene="mrx(A)"
                     /locus_tag="ACHUPM_002856"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFR0870581.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(10134..11039)
                     /locus_tag="ACHUPM_002857"
     CDS             complement(10134..11039)
                     /locus_tag="ACHUPM_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR0870582.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            complement(11161..>11287)
                     /locus_tag="ACHUPM_002858"
     CDS             complement(11161..>11287)
                     /locus_tag="ACHUPM_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFR0870583.1"
                     /translation="TAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM"
BASE COUNT         2361 a         3253 c         3313 g         2360 t
ORIGIN      
        1 cttacgccac catcaaaggt attgaggtga tgcgtgcact acgcaaaggc caggcctcag
       61 cattttatta tggtgatccc ctgggcgaaa tgcgcctggt aagcagagtt tttgaaatgt
      121 aaggcctttg aataagacaa aaggctgcct catcgctaac tttgcaacag tgcccccaat
      181 gcgctgtcca gtcatggaat tccctgcaaa atgtcaggga agactctatg accttcaacg
      241 agatatgtca ataaattcaa aattcaatcc tatcctgacg caatttacac atggcatctg
      301 acatcaggtt agggtatgcc tcaacctgac ggcggcgaat cacaagcgtc cggtttgacg
      361 ctggtttcgg tcgcagtgct ggcccgcgcc tggaagcgct gatagcactc cagcccgcag
      421 aaatgctcca cgtactcggc cccttccggc gtgaaggcgg catcgagcgg gatttccttg
      481 cagcacacgc agcaactggt gctcggttca ttggcgttca tggtggtgtt cctccattgg
      541 ttgacgaagc cgacgaaggc cgccgccggc atcggcctgg cgaacaggaa accctgcacc
      601 gtgtcgcaac ccgcctgccg caaccacgca aggcagtcgg gtgtttccac accctcggct
      661 accacctcca ttgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact
      721 atagcagcgg aggggttgga tccatcaggc aacgacgggc tgctgccggc catcagcgga
      781 cgcagggagg actttccgca accggccgtt cgatgcggca ccgatggcct tcgcgcaggg
      841 gtagtgaatc cgccaggatt gacttgcgct gccctacctc tcactagtga ggggcggcag
      901 cgcatcaagc ggtgagcgca ctccggcacc gccaactttc agcacatgcg tgtaaatcat
      961 cgtcgtagag acgtcggaat ggccgagcag atcctgcacg gttcgaatgt cgtaaccgct
     1021 gcggagcaag gccgtcgcga acgagtggcg gagggtgtgc ggtgtggcgg gcttcgtgat
     1081 gcctgcttgt tctacggcac gtttgaaggc gcgctgaaag gtctggtcat acatgtgatg
     1141 gcgacgcacg acaccgctcc gtggatcggt cgaatgcgtg tgctgcgcaa aaacccagaa
     1201 ccacggccag gaatgcccgg cgcgcggata cttccgctca agggcgtcgg gaagcgcaac
     1261 gccgctgcgg ccctcggcct ggtccttcag ccaccatgcc cgtgcacgcg acagctgctc
     1321 gcgcaggctg ggtgccaagc tctcgggtaa catcaaggcc cgatccttgg agcccttgcc
     1381 ctcccgcacg atgatcgtgc cgtgatcgaa atccagatcc ttgacccgca gttgcaaacc
     1441 ctcactgatc cgcatgcccg ttccatacag aagctgggcg aacaaacgat gctcgccttc
     1501 cagaaaaccg aggatgcgaa ccacttcatc cggggtcagc accaccggca agcgccgcga
     1561 cggccgaggt cttccgatct cctgaagcca gggcagatcc gtgcacagca ccttgccgta
     1621 gaagaacagc aaggccgcca atgcctgacg atgcgtggag accgaaacct tgcgctcgtt
     1681 cgccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac
     1741 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaaact
     1801 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     1861 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg tacagtctat
     1921 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     1981 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttagcca ttaagggagt
     2041 taaattgaaa atatcattga tttctgcagt gtcagaaaat ggcgtaatcg gtagtggtcc
     2101 tgatatcccg tggtcagtaa aaggtgagca actactcttt aaagcgctca catataatca
     2161 atggctcctt gtcggaagaa aaacatttga ctctatgggt gttcttccaa atcgcaaata
     2221 tgcagtagtg tcaaagaacg gaatttcaag ctcaaatgaa aacgtcctag tttttccttc
     2281 aatagaaaat gctttgaaag agctatcaaa agttacagat catgtatatg tctctggcgg
     2341 gggtcaaatc tataatagcc ttattgaaaa agcagatata attcatttgt ctactgttca
     2401 cgttgaagtc gaaggtgata tcaaattccc tataatgcct gagaatttca atttggtttt
     2461 tgaacagttt tttatgtcta atataaatta tacataccag atttggaaaa aaggctaaca
     2521 atgcgttgca gcaccagtcg cttcgctcct tggacagctt ttaagtcgcg tctttgtggt
     2581 tttgctgcgc aaaagtattc cacaaagccg caacttaaaa gctgccgctg aacttaacgt
     2641 taggcatcat gggtgaattt ttccctgcac aagttttcaa gcagctgtcc cacgctcgcg
     2701 cggtgatcga gcgccatctg gctgcgacac tggacacaat ccacctgttc ggatctgcga
     2761 tcgatggagg gctgaagccg gacagcgaca tagacttgct cgtgaccgtc agcgccgcac
     2821 ctaacgattc gctccggcag gcgctaatgc tcgatttgct gaaagtctca tcaccgccag
     2881 gcgatggcgg aacatggcga ccgctggagc taactgttgt cgctcgaagc gaagtagtgc
     2941 cttggcgcta tccggcgcgg cgtgagcttc agttcggtga gtggctccgc cacgacatcc
     3001 tttccggaac gttcgagcct gccgttctgg atcacgatct tgcgattttg ctgaccaagg
     3061 cgaggcaaca cagccttgcg cttctaggcc catccgcagc cacgtttttc gagccggtgc
     3121 cgaaggagca tttctccaag gcgcttttcg acactattgc ccagtggaat gcagagtcgg
     3181 attggaaggg tgacgagcgg aacgtcgttc ttgctcttgc tcgcatttgg tacagcgctt
     3241 caactggtct cattgctcct aaggacgttg ctgccgcatg ggtatcggag cgtttgcctg
     3301 ccgagcatcg gcccctcatc tgcaaggcac gcgcggcgta cctgggtagc gaggacgacg
     3361 acctagcaat gcgcgtcgaa gagacggccg cgttcgttcg atatgccaaa gcaacgattg
     3421 agagaatctt gcgttgagcg gcatgtgcga aaagtgcatc gacccgcgcc gagggcatct
     3481 gatgcctaac tcggcgttca agcggacggg ctgcgcccgc cgctcaacta tgcgttagat
     3541 gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt
     3601 taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg ctggcttttt
     3661 cttgttatcg caatagttgg cgaagtaatc gcaacatccg cattaaaatc tagcgagggc
     3721 tttactaagc ttgccccttc cgccgttgtc ataatcggtt atggcatcgc attttatttt
     3781 ctttctctgg ttctgaaatc catccctgtc ggtgttgctt atgcagtctg gtcgggactc
     3841 ggcgtcgtca taattacagc cattgcctgg ttgcttcatg ggcaaaagct tgatgcgtgg
     3901 ggctttgtag gtatggggct cataattgct gcctttttgc tcgcccgatc cccatcgtgg
     3961 aagtcgctgc ggaggccgac gccatggtga cggtgttcgg cattctgaat ctcaccgagg
     4021 actccttctt cgatgagagc cggcggctag accccgccgg cgctgtcacc gcggcgatcg
     4081 aaatgctgcg agtcggatca gacgtcgtgg atgtcggacc ggccgccagc catccggacg
     4141 cgaggcctgt atcgccggcc gatgagatca gacgtattgc gccgctctta gacgccctgt
     4201 ccgatcagat gcaccgtgtt tcaatcgaca gcttccaacc ggaaacccag cgctatgcgc
     4261 tcaagcgcgg cgtgggctac ctgaacgata tccaaggatt tcctgaccct gcgctctatc
     4321 ccgatattgc tgaggcggac tgcaggctgg tggttatgca ctcagcgcag cgggatggca
     4381 tcgccacccg caccggtcac cttcgacccg aagacgcgct cgacgagatt gtgcggttct
     4441 tcgaggcgcg ggtttccgcc ttgcgacgga gcggggtcgc tgccgaccgg ctcatcctcg
     4501 atccggggat gggatttttc ttgagccccg caccggaaac atcgctgcac gtgctgtcga
     4561 accttcaaaa gctgaagtcg gcgttggggc ttccgctatt ggtctcggtg tcgcggaaat
     4621 ccttcttggg cgccaccgtt ggccttcctg taaaggatct gggtccagcg agccttgcgg
     4681 cggaacttca cgcgatcggc aatggcgctg actacgtccg cacccacgcg cctggagatc
     4741 tgcgaagcgc aatcaccttc tcggaaaccc tcgcgaaatt tcgcagtcgc gacgccagag
     4801 accgagggtt agatcatgcc tagcattcac cttccggccg cccgctagcg gaccctggtc
     4861 aggttccgcg aaggtgggcg cagacatgct gggctcgtca ggatcaaact gcactatgag
     4921 gcggcggttc ataccgcgcc aggggagcga atggacagcg aggagcctcc gaacgttcgg
     4981 gtcgcctgct cgggtgatat cgacgaggtt gtgcggctga tgcacgacgc tgcggcgtgg
     5041 atgtccgcca agggaacgcc cgcctgggac gtcgcgcgga tcgaccggac attcgcggag
     5101 accttcgtcc tgagatccga gctcctaggg atcgcctcag aaaacggaaa ataaagcacg
     5161 ctaagccgta agtaagcgtg ctcctgtgaa agccacagct aaaactgcgt agtacacata
     5221 gaggtatttt tttctagatt tatatcgaat ttcgttatcg aatttcgctt attattgatc
     5281 aactcgttgc agatcatgaa ggcgaaagta tgacgaataa ccctaccgat gacagcagac
     5341 catggtcggt ctttcttatt tttctgcggc ttggattgac atcttttggc ggccccattg
     5401 cgcacttggg ctacttccgc gccgaatttg tcacacggcg gcgctggctc tccgaacgga
     5461 gctatgctga cttggtcgcg ctttgtcagt tcttgccagg gcctgcaagc agccaggtcg
     5521 gcatagcggt aggactgtct cgggctggat acagcggggc gctggctgct tgggctggct
     5581 tcacgctgcc gtctgccata gccttgatcc tttttgcgct cggcatctcc agctatggcg
     5641 attacgtctc gcagggcgcg ttgcatggct taaaagtggt ggctgtggcc gtggtcgctc
     5701 aagcagtatg gggcatggcg cgtaacctat gcacggatgg gctgcgagtc accatcatgg
     5761 caattgctac ctgcgtcgtt ttacttgtgc cgtccgcgtg gggacaggtt ggcgtgattg
     5821 ctatcgcagg catcgcaggc cggttattgt tcaagccagc gaaagttgtt gagcatgacc
     5881 ccctacctat cacggtcagt caccgggccg gcgtgctttg gctctcgctg ttctttgtct
     5941 tgctgattgg cctgccggtg ttggccgaac tgatgccaag tcaaaccatg gcaatggtgg
     6001 attccttcta tcgtgtcgga tcactggtgt tcggcggtgg tcacgttgtg ctgccattac
     6061 tgcaagccga agtggtgccc tccggctggg tcaacaatga atcctttctc gcggggtacg
     6121 gggcagctca agcggtgccc ggccctttgt tcacgttcgc cgcgtttctt ggtgcctcga
     6181 tgaacaccgc cccgtcgggc tggatcggcg gcattgtgtg tctgctggct atcttcgcgc
     6241 cctcgttctt gctggtcgtc ggatcaatgc cattttggga gcgtttgcgc cgcaatacag
     6301 gcatccaagc tgcgctggcc gggatcaatg ccgctgtagt cggcttgctg ctggccgcgc
     6361 tgtatcagcc tgtatggact agcgccatct ttcagccgca agacttcggc ttggcattag
     6421 ttgcccttgt cgcacttatg ttctggaagc tcccgccgtg gctggttgtc ctcggtagcg
     6481 gtgcagctgg gtggctgttg agcgtcgctc tgtgaggcac aaaaaatgac tgacaaagac
     6541 ctctacggcg gtttgatccg cctgcacatc cttcaccatg cagccgagga acctgtcttt
     6601 gggctgggga tcatcgaaga gctacgccga cacggctacg agatgagcgc tggcaccgtg
     6661 tacccgatgc tgcacggcct ggaaaaaaaa ggctatctga cctcacgcca cgaacgcacc
     6721 gggcgacgtg aacggcgtgt ttatgacatc actgaacaag gcagaactgc acttgctgat
     6781 gcgaaaacaa aggtcaagga gctgttcgga gagttggtag aaggtggttg agtttttgct
     6841 tctatgtcag gttgagctat accctaactg gattgtcatt ttcagaagac gactgcacca
     6901 gttgattggg cgtaatggct gttgtgcagc cagctcctga cagttcaata tcagaagtga
     6961 tctgcaccaa tctcgactat gctcaatact cgtgtgggct ctgttgcaaa aatcgtgaag
     7021 cttgagcatg cttggcggag attggacgga cggaacgatg acggatttca agtggcgcca
     7081 tttccagggt gatgtgatcc tgtgggcggt gcgctggtat tgtcgctatc cgatcagcta
     7141 tcgcgacctt gaggaaatgc tggcggaacg cggcatttcg gtcgaccata cgacgatcta
     7201 tcgctgggtc cagtgctacg ccccggagat ggagaagcgg ctgcgctggt tctggcggcg
     7261 tggctttgat ccgagctggc gcctggatga aacctacgtc aaggtgcggg gcaagtggac
     7321 ctacctgtac cgggcagtcg acaagcgggg cgacacgatc gatttctacc tgtcgccgac
     7381 ccgcagcgcc aaggcagcga agcggttcct gggcaaggcc ctgcgaggcc tgaagcactg
     7441 ggaaaagcct gccacgctca ataccgacaa agcgccgagc tatggtgcag cgatcaccga
     7501 attgaagcgc gaaggaaagc tggaccggga gacggcccac cggcaggtga agtatctcaa
     7561 taacgtgatc gaggccgatc acggaaagct caagatactg atcaagccgg tgcgcggttt
     7621 caaatcgatc cccacggcct atgccacgat caagggattc gaagtcatgc gagccctgcg
     7681 caaaggacag gctcgcccct ggtgcctgca gcccggcatc aggggcgagg tgcgccttgt
     7741 ggagagagct tttggcattg ggccctcggc gctgacggag gccatgggca tgctcaacca
     7801 ccatttcgca gcagccgcct gatcggcgca gagcgacagc ctacctctga ctgccgccaa
     7861 tctttgcaac agagcccgcc gtgctagtct gctcggtgat ggtggagtga agccaacccg
     7921 caatcgggtt atgaatctgc atcgcgattc gcaatcagct gtctcttgag catgtcgaac
     7981 tcctgcgatg ttatctcgcc gtgctcgtgc tgctacacca acttgtccaa ctcatccgcc
     8041 acaccgctcc ccaccacctg cgatggccag gttgtttgct gggcgcattg gcggagtatc
     8101 gtctcgaccc gggacaccca caccggcaca accttacggg agtgattcac tgtcaaagaa
     8161 tcggcccggt gctctgacgc aagtatcggg atggtcacca tttgtaagcc gtagacctga
     8221 gtggtgatca agacttcgat accaccgacc gtaccggtac taatcgacga cggtcgtgtt
     8281 cgtcgcctgc cgcagggact ctgcacacct ccgtttacgc atgtgcctgg aggagttgga
     8341 aatcgtcgtg ttcgggaaac attaaacaca ggatggcagc gatctgagcc agcacatgat
     8401 cagctagctc accatccgga tcgacggccc actgcatcgt cgcgccagcg atgaccgagt
     8461 gcaggagcaa ctcagctgcc gcaggagcac ctgggggcag tcgcttgcgg atcccctcca
     8521 ccaccgcgcg gttccgctgg atcgcaagcg tgcgtagctc cggcacctgg agctcgtacc
     8581 aggagatgag atagttcacc gagaagtcgt tgcgagtgtt catgctccga acgagcacct
     8641 gcaaaaattc ccagagccct tgcggccctg cgcctatcgg tatcgcattc aggtaatgcc
     8701 gcacctgctc gacgccgcgc tccatcatcc tcaccagcag cgtatcgcgg ttggtgaagc
     8761 gctggattaa cgctgcgcgg gagagcccca cctcctttgc tactccgctg agcgtgaact
     8821 ctatgggacc gcaacgcttc agcactacgg tggcggcctc gagtacctcg tcatcggact
     8881 tgagcttggg gcggggcatc agtgttcacc ttctgtatgg gttggggcgg aggctgtggc
     8941 tgccgccgcc attgtagcaa attgaagacg gagcgagagt agagccacga gccccgcaaa
     9001 cacggccgat acaacgaggc cagggagcgg accagcaagg tcgacaaacg cgccggccgc
     9061 aagcataacc atgggcgagg ctgacagcat caccgccgag accgtgccga gtacccggcc
     9121 gagaagttct ggcggcgtgc ggttgtagat ggcagcgttg agaatgggag agactgagcc
     9181 ggtcagcagt cccacgagcg cgcccaacaa catcagcacc ggcacgcctg gcaactgtga
     9241 aagcagaagc gagcccaccg cagagccaca aaatgccacc gccagccagt tctgcgctga
     9301 tatccgggcg ccgaccgacg catgaatggc aatgccaagg agaccaccag cccccatcat
     9361 tgaggagaac agcccgagct ctgctacttg gcgtcctgca tctacaaaca gcgcaggcat
     9421 gatgacgctg ccgttggcgc caacgatgcc cacgaagatc atcactatac caaagagagg
     9481 gcgcagcagg ggttcgctcc agagaaaagc gacgccggcg cgcatggaga gagtcgccgt
     9541 cgtggtcatc gtccgagcgg cacgcgcggg aagcacccac gcgccgagca gacctgcaag
     9601 gacggagcag aacgccgtca gcccgagcgt tggcgcagcg ccaagcaggc cgattgcggc
     9661 ccccccaagg gccgggccac ctagaatcgc gacgttcccg atcaccgctt tcagtgacga
     9721 gacgcgctca acggagagcc cggcgacgtg gccgagtttg ggcagctcac tgtcctgcgc
     9781 ggccataccg ggtgcgtcga acgcggcacc gagcaccacg caagcgatca gcccagtgtt
     9841 cgagagggcg ccaacggcat cgagcagtgg gatgctcgcc atggccacgc cgcccaccac
     9901 acccgagatc aatgcgacgg gcgcgcgccc gaaccgatcg acgaggccac caccaaccca
     9961 cgcgccgatg atggtcgcga tgacgctgct agcggccgtg gcgcccgccc aggccgcgct
    10021 ctttgtatga gacaggacga accatggaag cgcgagggcc gccaccgcgt tgccgatccg
    10081 gaagagaaag gtcgccgcga acagcgtcgc gagcgggcta tatcgacgtt cgctcattcc
    10141 gctgcggcga gctgcgcctt cgccgcagcg aggtactctt cgttacccga gtcgagggcg
    10201 aagagtgcgt aggtgaccgc cccgaacgca aggcgctccg cgatgtggtg ggcgagccgc
    10261 ggccacaccc ggccaccggc cgcttcatac gtgaggagga gcttcgcgag cccctcttca
    10321 ccaaagacca taaggtgcgc ggccatgtcg atggcagggt catcaacgcg ggcctcgctc
    10381 cagtcgatca tcccgctgac gcgctccgtg ttgtcgatga gcacatggcc cacgtagaga
    10441 tcgccatgca ccaccacgga gaaatctggc cacgacgaat cgtcgtcgag ccagcgctgc
    10501 caccggtgga ggcgcttgtc gttcaccacg aactcgcgtc ggacgcggtc aacgtcgtcg
    10561 gccaccttct gacgggcctg cgtcggtgta cggatgagca tccccgcatc cacggcggcg
    10621 gaaatgggga cggcatgcag ggcggcgagc gcggtcgcga agctctccgc gaagacctcc
    10681 gagtcctgcg gcacgaccca gtcgggcgtg gacgaaccag gctggatgac catcgcagtc
    10741 gagtcttcga gcatgggata ggcaacgagc tcggcgttgg ccacgcgcca gtccggcacc
    10801 gcgaacggca ggcgattctt gagcattgcc agcacccgcg cctctggttc gaccttcgcg
    10861 cttacctcgg ctcggcgcgg gatgcgcagc acccaccgac gtccatcgtc gacggtggcg
    10921 atcacgatcc tatagtcgag cccaagctca ttgacagtca gcgggccatg gagcttgagc
    10981 ccatgtcggg ctgcaagtgc gtacagttgg gaggtatcgg cggtcgtgac tacggtcatg
    11041 attcactcct gagggcttga cgggtttagc cacctaaatg taacagtcac gtcggttata
    11101 ttcaatccgg cactgttgca aagttagcga tgaggcagcc ttttgtctta ttcaaaggcc
    11161 ttacatttca aaaactctgc ttaccaggcg catttcgccc aggggatcac cataataaaa
    11221 tgctgaggcc tggcctttgc gtagtgcacg catcacctca atacctttga tggtggcgta
    11281 agccgtc
//
LOCUS       DAZPAC010000128        11242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZPAC010000128 DAZPAC010000000
VERSION     DAZPAC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.128"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            3..308
                     /locus_tag="ACHUPM_002859"
     CDS             3..308
                     /locus_tag="ACHUPM_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR0870584.1"
                     /translation="MLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFF
                     DQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            507..788
                     /gene="ppiC"
                     /locus_tag="ACHUPM_002860"
     CDS             507..788
                     /gene="ppiC"
                     /locus_tag="ACHUPM_002860"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR0870585.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="ACHUPM_002861"
     CDS             complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="ACHUPM_002861"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR0870586.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2500..3393
                     /gene="ilvY"
                     /locus_tag="ACHUPM_002862"
     CDS             2500..3393
                     /gene="ilvY"
                     /locus_tag="ACHUPM_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR0870587.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="ACHUPM_002863"
     CDS             complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="ACHUPM_002863"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR0870588.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="ACHUPM_002864"
     CDS             complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="ACHUPM_002864"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR0870589.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="ACHUPM_002865"
     CDS             complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="ACHUPM_002865"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR0870590.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="ACHUPM_002866"
     CDS             complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="ACHUPM_002866"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR0870591.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8116..9762)
                     /gene="ilvG"
                     /locus_tag="ACHUPM_002867"
     CDS             complement(8116..9762)
                     /gene="ilvG"
                     /locus_tag="ACHUPM_002867"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR0870592.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9765..9815)
                     /gene="ilvX"
                     /locus_tag="ACHUPM_002868"
     CDS             complement(9765..9815)
                     /gene="ilvX"
                     /locus_tag="ACHUPM_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR0870593.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="ACHUPM_002869"
     CDS             complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="ACHUPM_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR0870594.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10353..11242
                     /locus_tag="ACHUPM_002870"
                     /pseudo
     CDS             10353..11242
                     /locus_tag="ACHUPM_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT         2554 a         3090 c         2950 g         2648 t
ORIGIN      
        1 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
       61 gcgatcatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
      121 acctgcaatt ctttgatcag cagattcgtt gggccaaagc cgacctagta caacaagtca
      181 gtcgaaaccg attaaacaga gcaaggactt acagagggta tcttaatcaa tgtttgccac
      241 atgagttggt tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atatcgctca
      301 taaattgaca ataaaataac catccccgat aatgactttg tagcccatag aggccctaag
      361 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac
      421 cagcccctcc ctgactaaca attacgttag catctgcgga tcatgctaaa atcgccgcct
      481 gacaattatt cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
      541 taaaagaaga gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg
      601 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
      661 aattccgcca gggtcagatg gttccggcgt ttgataaagt tgtgttctct tgtccggtac
      721 tggagccaac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
      781 gtaactaata gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt
      841 gggagagggt cggggtgagg gcatcagcgc gcacttaacc cgcaactgca atacgtttca
      901 tatccgtcat atagccgcgc agtttcttac ctacctgctc aatcgcatgg cagcgaatcg
      961 cttcgttcac atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
     1021 gatcgcccgg ttgcagctct gccataaacg gtttcagcag cggcacacaa gcgtaagaga
     1081 acagatagtt accgtactcg gcggtatcag agataaccac gttcatttcg tacagacgct
     1141 tacgggcgat ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt
     1201 cttcgatgat gccggaatca accatggttt caaacgccag ctcaacgccc gctttcacca
     1261 tcgcgatcat cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
     1321 gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
     1381 catcgttggc ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca
     1441 tatgtttctg gaacaggggt gccatgatct ctttcagctg ttcagacagc gcgtaagcac
     1501 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg
     1561 cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc
     1621 cctcttccac cagcttgtcg aagcacagca gagagccagc ctgcaacata ccgcacagga
     1681 tggtttgctc acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc
     1741 ccgcacggtg accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt
     1801 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
     1861 cttcacgtac ttcggtgcca gggcatttcg gcgcaaccat gactacggta atgtctttac
     1921 ggatctgctc gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt
     1981 ctttcatcag tggctgtacg gtgcgcacta catcagagtg ctgcttgtct ggcgtcaggt
     2041 taaccaccag atccgcctgc gggatcagtt cttcgtaagt accaacttta aaaccgtttt
     2101 cggtcgcttt acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
     2161 agatatcgag accagaatca cgcatgttca ggccctggtt cagcccctgt gcgccacagc
     2221 cgacgataac cactttttta ccctgaaggt agctcgcgcc gtcggcgaat tcgtcgcgcc
     2281 ccataaagcg acatttgccc agctgtgcca gctgctggcg caggttcagt gtattgaagt
     2341 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga
     2401 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac
     2461 attgcaattt ctgcaacgtc aacatcgagg gctgcgccat tggatttacg cgatctgaaa
     2521 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
     2581 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta
     2641 tttgtgcgcg ataaccgcac ggtaacgctg acggaagcgg gcgaagagct gcgcgttttc
     2701 gcccagcaaa cgctgttgca gtatcagcaa ttgcgccaca ccatcgatca gcaagggccg
     2761 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg
     2821 ccgattctgg atcgcttccg cgcggaacac ccgtcggtgg agatcaaact caccactggt
     2881 gatgcggcag atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg
     2941 aaaccggaga cgctacccgg cgctgtggcg ttttcgatgc tggagaatct ggcagtagtg
     3001 ctgattgccc ccgcgctgcc ctgcccggtg cgtaatcagg tatctgtcga aaagcccgac
     3061 tggtcaacgg tgccgtttat tatggccgat caggggccgg tacgccgccg cattgaactg
     3121 tggtttcgac gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa
     3181 gcgatggtat cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtggtgctg
     3241 gaaaacagcc cggagcctgt acgcaaccgg gtaatgattt tagatcgcag cgatgagaaa
     3301 acgccgtttg aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag
     3361 gcgttctgga ggattttgcc gaaccacaaa tgacgttgtc gcgcgggtag gcctgataag
     3421 cgaagcgcta tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccggattatt
     3481 ggtttcgtcg tggcaatcgt agcccagctc atttagccgg gtttcgaaat ccggttcatg
     3541 gtcgccgagt tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg
     3601 gaacaaagaa atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
     3661 tgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc
     3721 gaccatatag cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta
     3781 gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg
     3841 cacgccgaca aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac
     3901 cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt
     3961 caccgccaac aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
     4021 gccgtggaag ttcacgttgg caccggaaag aatatgcgcc aggcgttcgc cgcgaatgtt
     4081 gtgctgagcg atatattttt tcattcccgc cagcgccaac gcgccagagg gttccgccac
     4141 tgcgcgcaca tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt
     4201 gatgatgtcg tcgagatact cctggcataa acggaaggtt tcgtcgccga tgcgttttac
     4261 cgcgacacct tcggcgaaca gtcccacgcg cggcagatca accggatgac ccgcatccag
     4321 cgctgctttc aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgtggcat
     4381 cagttgtttg atcagcaccg caacgcccgc cgccagaccg ccgccgccga ctggcacaaa
     4441 tacgcggtcg agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc
     4501 aatcaccatc ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc
     4561 gatcgcttta gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa
     4621 tccacgtacc gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt
     4681 cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt
     4741 gatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg
     4801 cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag
     4861 acgcgacgac agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg
     4921 cgcgcgcagc actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga
     4981 gtcagccatt attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg
     5041 gttgccaggc tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc
     5101 gtccaggctt tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg
     5161 cttacctgta actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca
     5221 atcagaccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca
     5281 gaggtgccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt
     5341 gatttcagga agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct
     5401 tcatagcgga tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc
     5461 gcgtcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg
     5521 acgcccgctg ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg
     5581 ccgccgtctt tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca
     5641 tcgagagaat cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg
     5701 cctgcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
     5761 agagtttgcg gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg
     5821 cgatccagtt caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
     5881 tatttttggg tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg
     5941 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc
     6001 gtgttggtag agccgcccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg
     6061 ctagcaatat tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat
     6121 tcaacaatgc gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc
     6181 agcgagccgt tgcctggctg cgacaaaccc agcgcttcgg tcaggcagtt cattgagtta
     6241 gcggtaaaca tcccggagca ggaaccgcag gttgggcagg cggaacgttc aacctgatcg
     6301 ctctgggagt cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagc
     6361 ttgatgatct gctcggacag tttggttttc ccggcttcca tcgggccgcc ggaaacaaag
     6421 atcaccggaa tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag
     6481 ttagagatgc agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca
     6541 gcgatcagtt cgcgagatgg cagtgaataa agcatccccc cgtggcccat agcaatccca
     6601 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
     6661 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
     6721 ttcacaaccg cgataatcgg cttgccaaaa tcggcatcgg tcattccggt ggcgcgccac
     6781 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
     6841 atactttatt tactcccagt gtctgtctcg taaatgggac ggtaagtgcc gtcccatttt
     6901 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa
     6961 gaggccgaag aaggcttgct gaatgcattt ggtaaccggg ccgcaacggc cttcacccac
     7021 ctgaataccg tctacactgc gcactggcgt gatttctgcc gccgtaccgg acataaacac
     7081 ttcatccgcc aggtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag
     7141 ctctttcgcc agtttgatga tggcatcacg ggtaataccc ggcagcgcgg atgaggtaaa
     7201 cggtggggtg aacagcacgc cgtctttcac ttcaaacagg ttttcacctg cgccttcaga
     7261 gatgtaaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct
     7321 acccaccagc agggaagaga ggtagttacc accggctttt gccgccgtcg ggatggtgtt
     7381 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
     7441 gcccagatac gctccccacg ggaaagcagc gataatcacg tcggttgagt atcccgctgg
     7501 cgggtttact cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt
     7561 gagattattt ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgcga
     7621 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
     7681 gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaagtgcc
     7741 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
     7801 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
     7861 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
     7921 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
     7981 tatttatatt ttgtgcatcg ctggcggcgg ccatattcat tgagcagacg tggaaaccac
     8041 gatgacgcac cacgcgtaaa acacgttcta aggtttctgg attgaagcga gccgatacat
     8101 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg cgccaggcgg
     8161 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
     8221 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc
     8281 agggatgccg aaggcgctgg ctaacatgag gaaatcgggg ttatcagtaa gggtggtttc
     8341 gctgtatcgt tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt
     8401 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac
     8461 attcatcatg aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac
     8521 ttgtgcgcca accgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat
     8581 gaaattttcc gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac
     8641 atctgtggtc acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg
     8701 cgcgtagata gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc
     8761 gcagtattgc tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt
     8821 taaatcaccc tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat
     8881 atccatatgg ataacactgg cgtgtggtgc gaaagtattc agtttgccgg tcacccggtc
     8941 atcaaaacgt gcgcccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc
     9001 tttgatgccg tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc
     9061 cagccctttc agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc
     9121 cggaactgcc tgcgccatac ccacgccacc gccaacgtac agcatcggtt tttgcgcttt
     9181 tgccagcatc tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac
     9241 ggtggtgaac cacggttcca ggtcaccgct ggctaactgg atatcttttg ggatatcgac
     9301 cagaaccgga ccaggacgac ctgagcaggc aacgtcgaat gcttcagcca tgatgcgcgg
     9361 caactcttcc agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcctag
     9421 gacatccact tcctgaaatg cgtcagtgcc gataaacggt gcggacactt gaccggtgat
     9481 ggcaacaaca gggatggaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc
     9541 gcccggacca gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat
     9601 agccgccatt gccgcaccct gctcatgtcg gcatagcaag tgctccacgc cgccgtcata
     9661 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
     9721 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
     9781 aatcttgaga aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta
     9841 aggtcaaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
     9901 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
     9961 taatcacgac taggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    10021 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg
    10081 gcctattttt atgatagctt taataatttc aatagaagtt gttgtttttt aaaaagaaaa
    10141 tagtgagtga aaaaaattca atttcagtat aaatgttgtt cccttcctcg tagttcatcc
    10201 tttgtccgct gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc
    10261 acaaataaac aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc
    10321 ttatgcacat aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg
    10381 cagccctggg agtaaatgcg ccccctgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg cgcataacgg cg
//
LOCUS       DAZPAC010000129        11202 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZPAC010000129 DAZPAC010000000
VERSION     DAZPAC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11202
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.129"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1285
                     /gene="narU"
                     /locus_tag="ACHUPM_002871"
     CDS             <1..1285
                     /gene="narU"
                     /locus_tag="ACHUPM_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR0870595.1"
                     /translation="KTITYLSDTGCLEIQGASLAVTVNLNKIGFNFTTDQLFLLTALP
                     SVSGALLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALL
                     CGFAGANFASSMGNISFFFPKAKQGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFL
                     GVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLH
                     LWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISD
                     KFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQM
                     IAVIFRQITIYRVKMKGGSDEQAQKEAITETAAALGFISAIGAVGGFFIPQAFGMSLN
                     MTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK"
     gene            1367..5107
                     /gene="narZ"
                     /locus_tag="ACHUPM_002872"
     CDS             1367..5107
                     /gene="narZ"
                     /locus_tag="ACHUPM_002872"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFR0870596.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5104..6648
                     /gene="narH"
                     /locus_tag="ACHUPM_002873"
     CDS             5104..6648
                     /gene="narH"
                     /locus_tag="ACHUPM_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0870597.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6648..7343
                     /gene="narW"
                     /locus_tag="ACHUPM_002874"
     CDS             6648..7343
                     /gene="narW"
                     /locus_tag="ACHUPM_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR0870598.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7340..8020
                     /gene="narI"
                     /locus_tag="ACHUPM_002875"
     CDS             7340..8020
                     /gene="narI"
                     /locus_tag="ACHUPM_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR0870599.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8099..8992
                     /locus_tag="ACHUPM_002876"
     CDS             8099..8992
                     /locus_tag="ACHUPM_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFR0870600.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9088..9933)
                     /gene="nhoA"
                     /locus_tag="ACHUPM_002877"
     CDS             complement(9088..9933)
                     /gene="nhoA"
                     /locus_tag="ACHUPM_002877"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFR0870601.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10106..10675
                     /locus_tag="ACHUPM_002878"
     CDS             10106..10675
                     /locus_tag="ACHUPM_002878"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFR0870602.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10679..10906)
                     /gene="pptA"
                     /locus_tag="ACHUPM_002879"
     CDS             complement(10679..10906)
                     /gene="pptA"
                     /locus_tag="ACHUPM_002879"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFR0870603.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(11006..>11202)
                     /locus_tag="ACHUPM_002880"
     CDS             complement(11006..>11202)
                     /locus_tag="ACHUPM_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0870604.1"
                     /translation="VVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKM
                     RKAAMGRNYLASVLTGSGLS"
BASE COUNT         2605 a         2917 c         2991 g         2689 t
ORIGIN      
        1 taagactatc acttatttaa gtgatactgg ttgtctggag attcaggggg ccagtctagc
       61 agttaccgtt aatctcaata aaatcggttt taattttact accgatcaac tctttttatt
      121 aaccgcatta ccctccgttt ctggcgcatt attgcgtgtt ccctactcct ttatggtgcc
      181 tatattcggt ggacgccgat ggacggtttt tagtactgca atcctgatta ttccttgcgt
      241 ctggctcgga attgccgtgc aaaatccgaa tactcctttt gggatattta tcgttatcgc
      301 tttgctatgc ggttttgcag gtgcaaactt tgcttcgagc atgggcaata tcagtttctt
      361 ctttccaaaa gccaaacaag ggagcgctct tgggattaat ggcggattag gaaacttagg
      421 tgtaagtgta atgcagctgg ttgcaccgct ggtcattttt gtacctgtat ttgcctttct
      481 cggcgtcaat ggcgtaccgc aggccgacgg ttcggtgatg tcgctggcga atgccgcatg
      541 gatttgggta ccgctactgg cgattgccac gatcgccgcc tggtcaggga tgaatgatat
      601 cgccagttca cgcgcctcaa ttgacgacca gctccctgtc ttacaacgcc tgcatctctg
      661 gctgctgagc ctgctttacc ttgccacctt cggttcgttt atcggttttt ctgcgggttt
      721 tgccatgctg gcaaaaaccc agttcccgga tgtgaatatt ctgcgcctgg cgttctttgg
      781 cccatttatc ggtgccatcg cgcggtcggt tggtggtgct atttccgata aattcggcgg
      841 cgtgcgggtg acgttgatca actttatttt tatggcgatt ttcagtgccc tgctgttcct
      901 taccttaccg ggcacaggct ccggtaattt catcgccttt tacgccgtat ttatggggct
      961 gtttctgact gcgggtctgg gaagtggttc tactttccag atgatcgccg tcatctttcg
     1021 ccagataacc atttatcggg tgaagatgaa aggcggtagt gatgagcaag ctcagaaaga
     1081 agccatcacc gaaacggcgg cggctctggg ctttatctca gccattggcg cagtgggcgg
     1141 cttttttatt ccgcaggcgt ttggcatgtc gctcaatatg accggctctc cggtcggcgc
     1201 gatgaaagtg tttttaatct tctacatcgt ttgtgtgctg ctgacctggc tggtttatgg
     1261 tcggcggaag ttcagccaaa aataagagat cgatgcctga tgtacaaccg tgtcgcatcc
     1321 gacaaggcat cagatcccaa ttcattcctg gagcaggagt catgtcatga gtaaactttt
     1381 ggatcgcttt cgctacttca aacaaaaggg cgaaaccttt gccgatggtc acggacaggt
     1441 gatgcatagc aaccgcgact gggaggacag ctatcgccag cgttggcagt tcgacaaaat
     1501 tgtgcgttcc acccacggtg ttaactgtac aggctcctgt agctggaaaa tctacgttaa
     1561 aaatggtctg gtgacctggg aaatccaaca gaccgactac ccgcgcactc gccctgacca
     1621 gcccaatcat gaacctcgcg gctgcccgcg tggcgcaagt tactcctggt atctctacag
     1681 cgctaaccgc ctgaaatacc cgctcattcg taaacgactg attgaactgt ggcgtgaagc
     1741 tctcaagcaa cacagcgatc cggtactggc gtgggcatcg attatgaacg atccgcaaaa
     1801 gtgcttaagc tacaaacaag tgcgtgggcg cggcgggttt atccgctcca actggcagga
     1861 acttaaccaa ctgattgccg ccgctaacgt ctggaccatc aaaatctatg gtccggatcg
     1921 cgttgccggt ttctcgccga tcccggcgat gtcgatggtg tcatacgctg ccggaacgcg
     1981 ttatctgtcg ctgttaggcg gcacctgttt aagcttctac gactggtatt gcgacctgcc
     2041 gcccgcctcg ccgatgacct ggggcgaaca aaccgacgtg ccggaatctg ccgactggta
     2101 taactccagt tacatcatcg cctgggggtc taacgtaccg cagacacgta cgccggacgc
     2161 ccacttcttt accgaagtac gttacaaagg caccaaaact atcgccatta cccctgacta
     2221 ctctgaagtg gccaaattgt gcgaccagtg gctggcaccg aaacaaggca ctgatagcgc
     2281 cctggcgatg gcaatgggcc atgtgatttt aaaagagttt catctcgata atcccagcga
     2341 ctactttatc aactactgcc gccgctatag cgacatgccg atgctggtaa tgctggaacc
     2401 tcgcgacgat ggtagctacg tacccgggcg catgatccgc gcgtctgacc tggtagatgg
     2461 actaggcgaa agcaataatc cgcagtggaa aaccgtggcg gttaataccg caggtgaatt
     2521 ggtagtgccg aatggttcga ttggtttccg ctggggagaa aaaggcaaat ggaatctgga
     2581 atccattgcc gccggtaagg aaaccgaatt gtcgttaacc ctgctcggtc aacatgacgc
     2641 tgttgcaggc gtggccttcc cctactttgg cggcattgaa aatccgcatt ttcgcagcgt
     2701 aaaacacaat ccggtgctgg tgcgccaatt gcccgttaaa aacctgacgt tagtcgatgg
     2761 caacacctgt ccggtggtca gcgtttatga tttggtgctg gcgaattacg gcctcgatcg
     2821 cgggctggaa gatgaaaaca gcgcgaaaga ttacgctgaa atcaaaccgt acaccccagc
     2881 ctggggtgag caaattaccg gcgtaccgcg ccagtatatt gaaaccatcg cccgcgaatt
     2941 tgccgatacc gcccataaaa cgcatggacg ctcaatgatt atcctcggcg ctggcgtcaa
     3001 ccactggtat cacatggata tgaactaccg tgggatgatc aatatgctga tcttctgcgg
     3061 ttgtgtcggg caaagcggcg gcggctgggc gcactatgtc ggtcaggaaa aactgcgccc
     3121 acaaaccggc tggttgccgc tggcctttgc gctcgactgg aaccgcccac cgcgccagat
     3181 gaacagtacc tcgtttttct acaatcattc cagccaatgg cgctatgaaa aagtctctgc
     3241 tcaggagtta ctttcaccgc ttgccgatgc cagtaagtac agcggtcatc tgattgattt
     3301 caacgttcgc gccgaacgca tgggctggct accttctgcg ccgcagctgg ggcgtaaccc
     3361 gctcgggatt aaagctgaag ccgacaaggc cggattatcc cccacagaat ttaccgccca
     3421 ggcgctgaaa tcgggcgatt tacgtatggc ctgcgaacaa ccagatagcg gcagcaatca
     3481 tccgcgtaat ttgtttgtct ggcgctctaa cctgcttggc tcctccggca aaggccacga
     3541 gtatatgcag aagtatctgc tggggaccga aagcgggatt cagggcgagg aactcggtgc
     3601 cagcgacggg atcaaaccgg aagaagtcga gtggcaaact gcggcgattg aaggcaagct
     3661 cgacctgctg gtgacgctcg acttccgcat gtccagtacc tgcctgttct ccgatatcgt
     3721 tctgcccacc gccacctggt acgaaaaaga cgatatgaac acctcggata tgcatccgtt
     3781 tattcatccg ctttctgcgg cggtcgatcc ggcgtgggaa tcacgcagcg actgggaaat
     3841 ctacaaaggt atcgccaaag cattttcgca agtgtgcgtg ggtcatcttg gcaaagaaac
     3901 cgacgtggta ttacaacccc tactgcatga ctctccggca gagctctcac agccgtgtga
     3961 agtgctcgac tggcgcaaag gcgaatgcga tctgattcca ggcaaaaccg cgccgaatat
     4021 tgtggcggtg gagcgcgact accctgctac gtatgaacgc tttacctcgc tcgggccatt
     4081 gatggacaaa cttggcaatg gtggtaaagg gatttcgtgg aatacccagg atgaaatcga
     4141 tttcctcggc aaactcaatt acaccaagcg tgatggcccg gcacaggggc gtccgctgat
     4201 tgacaccgcc attgacgctt cagaagtgat tctggcactg gcaccggaaa ccaacggtca
     4261 tgttgcagtc aaagcgtggc aggcgctggg cgagatcacc gggcgcgaac atacccatct
     4321 ggcgctgcac aaagaggacg agaagattcg ctttcgcgat attcaggcgc agccgcgtaa
     4381 aattatctcc agccccacat ggtccggtct ggaaagcgat cacgtctcct ataatgcggg
     4441 atacaccaac gttcatgagt taattccgtg gcgcacgctg tcgggacgcc agcagctcta
     4501 tcaggatcat ccgtggatgc gtgcttttgg tgaaagcctg gtggcttatc gcccgcctat
     4561 cgacacccgt agcgtcagtg agatgcgcca gatcccgcca aacggcttcc cggaaaaagc
     4621 acttaacttc ctgacgccgc accagaaatg gggcattcac tcaacctaca gtgaaaacct
     4681 gctaatgctg acgctctctc gcggtggacc gattgtctgg atcagcgaaa ccgatgcccg
     4741 agaactgacc attgtcgata acgactgggt ggaagtcttt aacgccaacg gcgcgctgac
     4801 ggctcgtgcg gtggtcagcc aacgtgtacc gccgggtatg accatgatgt atcacgccca
     4861 ggaacgcatt atgaatattc ctggttcgga agtaactggc atgcgcggcg gcattcataa
     4921 ctcggtcacc cgtgtttgcc cgaaaccgac gcatatgatt ggcggttacg cgcagctggc
     4981 ctggggcttt aactactacg gcaccgtcgg ctcgaaccgc gacgaattca tcatgatccg
     5041 caagatgaag aacgttaact ggctggatga tgaaggtcgc gatcaggtac aggaggcgaa
     5101 aaaatgaaaa tacgttcgca agtcggcatg gtgcttaacc tcgacaaatg tatcggttgc
     5161 catacctgtt cggtgacctg taaaaacgtc tggaccggac gcgaaggcat ggagtacgca
     5221 tggtttaaca acgtcgaaac caaaccgggc attggttatc cgaaaaactg ggaagatcag
     5281 gaagagtggc aaggcggctg ggtccgcgat gtgaatggca agatacgccc gcgtctgggc
     5341 agcaagatgg gcgtaataac caaaatcttc gccaacccgg tggtgccgca gattgatgat
     5401 tactacgaac ctttcacctt cgactacgaa catttgcata gcgcaccgga aggcaaacat
     5461 atccctactg ctcgcccgcg ttcgctgatt gacggtaagc ggatggacaa agtgatctgg
     5521 gggccaaact gggaagaact gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac
     5581 ttcgaggcca tgcaaaagga gatgtacggg cagtttaaaa acaccttcat gatgtacctg
     5641 ccgcgcctgt gcgaacactg cctcaatccc agttgcgtgg cgacctgccc aagcggcgct
     5701 atctacaaac gcgaagaaga cggcattgtg ctgattgatc aggataaatg ccgtggctgg
     5761 cgtttgtgca ttagcggttg tccgtacaaa aaaatctact tcaactggaa aagcggcaag
     5821 tcagaaaaat gtatcttctg ttatccgcga attgagtcag gccaacccac cgtgtgctca
     5881 gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatt
     5941 gaggaagcgg cgagcaccga gcgcgaagtt gacctctatg aacgtcagtg cgaagtgttc
     6001 ctcgatccac acgatccctc agtgatcgag gaagccctga aacaaggtat tccacaaaac
     6061 gtgattgaag ctgcccagcg ttcgcctgtc tacaaaatgg cgatggactg gaaactggcg
     6121 ctaccgctgc accctgaata tcgcaccctg ccaatggtct ggtacgttcc tccgctgtcg
     6181 ccgattcagt cctacgcaga tgcgggcggt ttgccgaaaa gcgaaggcgt gctgcccgcc
     6241 atcgaaagcc tgcgtattcc ggtgcaatat ctcgccaata tgttgagtgc cggcgatacc
     6301 ggtccggtac tgcgggcgct gaaacggatg atggcgatgc gccactatat gcgttcacaa
     6361 accgtggaag gcgttactga tactcgtgcc atcgacgaag taggcctgag cgtcgcccag
     6421 gtcgaagaaa tgtatcgcta tctcgccatt gccaactatg aagatcgttt tgttatcccg
     6481 accagccatc gggaaatggc gggcgatgcc ttcgcagaac gcaacggctg cggttttacc
     6541 tttggcgacg gttgccacgg ttcggacagt aaattcaacc tgttcaacag tagccgtatc
     6601 gatgccatca acatcaccga agtgcgcgac aaagcggagg gcgaataatg cagatcctca
     6661 aagtgatcgg cctgttgatg gagtatccgg atgagctgtt gtgggaatgc aaggaggacg
     6721 cgctggcgtt gatccgccgc gacgcgccga tgcttacgga tttcacccac aacctgctta
     6781 acgcgccgct gctggataaa caggccgaat ggtgcgaagt gtttgaccgc gggcgcacca
     6841 cgtcgctgct gctgttcgaa catgttcatg ccgagtcccg cgatcgcggc caggcaatgg
     6901 tggacctgct ggcggagtat gaaaaggtcg gcctgcagct ggattgtcgg gaactgcccg
     6961 attatctacc gctgtatctg gagtatttaa gcgtactgcc tgacgatcag gcgaaagaag
     7021 gattgctcaa tgttgcgccg atcctcgccc tgcttggcgg tcgcttaaaa caacgcgagg
     7081 caccgtggta cgcgttgttt gatgctctgc tgcaactggc gggaagtata ctttcaagtg
     7141 acagtgtcac aaaacaagta aacagcgaag agcgcgatga cacccgccag gcgcttgatg
     7201 cagtgtggga agaggaacag gtgaagttta ttgaagataa cgccacggcg tgtgacagct
     7261 cgccgcttaa tcaatatcag cgacgcttta gccaggatgt cgcgccgcag tatgtcgaca
     7321 tcagtgcggg aggtgggaaa tgattcagta tctgaacgtc tttttttacg atatctaccc
     7381 gtacatttgt gcgacggtgt ttttcctcgg cagctggctg cgctacgact acgggcagta
     7441 cacctggcgc gcctcctcaa gtcaaatgct cgataaacgc gggatggtga tatggtcgaa
     7501 tctgttccat atcggcattt tggggatttt cttcgggcac ttgttcggca tgttaacgcc
     7561 gcactggatg tacgcgtggt ttttgccagt ggcagcgaaa caactcatgg cgatggtgct
     7621 cggcggtatt tgcggtgtgt tgacgttgat tggcggcgca ggactgctgt ggcgcagtct
     7681 gacaaatcag cgcgtgcgtg ccacttccac tacgccggat atcatcatca tgagcatttt
     7741 gctgatccag tgtctgttgg ggctaagtac cataccgttt tcagcacagt atcctgatgg
     7801 tagcgaaatg atgaagctgg tgggttgggc gcaaagcatt gtcactttcc gtggtggatc
     7861 gtcagagatg ctcaacggcg tagcgttcgt cttccgcctg catctggtgt tgggaatgac
     7921 catcttcctg ctcttcccgt tcacccgact ggtgcatgtg tggagcgcgc cgtttgagta
     7981 ctttactcgt cgatatcaaa ttgtgcgttc gcggcgataa tttcaccctg gccggatagc
     8041 ccttcgctct ccggccactt gcaggctatg atagttctcc agtcacagga gaacgttaat
     8101 gaaaccgcag gtgtaccacg tcgatgcttt tacctctcaa ccatttcgcg gcaattctgc
     8161 cggtgttgtt ttccccgccg ataatcttag cgaagcacaa atgcagctta tcgcccgcga
     8221 gttaggccat tcggaaactg cttttctgct gcacagcgac gacagcgatg tgcgcatccg
     8281 ctactttacg ccaacggttg aagtgccgat ttgcggtcac gcgacagtag ctgcgcacta
     8341 tgtacgtgcg aaggtgttag gtttaggaaa ttgcacgatc tggcaaacat cgctggcagg
     8401 aaaacatcgc gtgactatcg aaaagcacaa cgatgattat cgtatttcgc tggaacaagg
     8461 tacgccgggc tttgaactgc cgctggaggg tgaagtacgc gcggcgatta tcaacgcatt
     8521 gcatcttact gaagacgata ttctgccggg tttgccgatc caggtcgcga cgaccgggca
     8581 ttctaaagtg atgatcccat tgaagccgga agtggatatc gacgctcttt cgcctgacct
     8641 taatgcgctg accgctatca gtaaacagat tggctgcaat ggtttcttcc cgttccagat
     8701 ccgtccaggc aaaaacgaaa ccgatggtcg tatgttctcg cctgcgattg gtattgtgga
     8761 agatccggtg actggcaatg ccaatggccc gatgggtgca tggttggtac atcacaacgt
     8821 attgccccac gatggcaacg tgttgtgtgt taaaggccat caggggcgcg cattggggcg
     8881 tgacggcatg attgaagtga cggtgacaat tcgtgataac caaccggaaa aagtcaccat
     8941 ttcaggcaca gcggtgattt tgtttcatgc cgaatgggca attgaaatct gaaatcgttg
     9001 cctgatgcgc ttcgcctgtc aggtctacaa ccgggctaat gtatgccgga taaggcgttt
     9061 acgccgcatc cggcaacctg acataaatta ttttcccgcc tccggatgcg tatcaaacgc
     9121 cgccatcacc agcgctaact catccacagt aaagccatgt ttcgcatcat ccacgcccag
     9181 accaaactgt tcctgcatca cggcatataa cgacgccaca tccggtagat ttcgctgctc
     9241 caccgcgtgc ccattttcat aatgggtaaa atgaaaattg gtcagtgtca gcttgccgcc
     9301 gtccggcaaa tggcggcaca tcagcaaatg atggcgaaaa tgcgactgcg gccagtgcgc
     9361 cgaccagaaa ttgcccatca cataatcgct ttgttgctgc tcgcagagat cgaagcggta
     9421 catcggctgc caatgctgat gatgattaaa ctgcaacacc caatcatcac cctcctgcaa
     9481 caaccgatac tctccgtgtg gcgtggtctg cacgagatcg gaaactaaac gaatcggcgc
     9541 ggttagcgtc tgcccaccga aaccgacatc agcaatccat ttttcccctt ccagttccac
     9601 caacagcaaa cgatgggtgc gcggcggtaa tgctggcgga tttgataaca ctacgcgccc
     9661 taacaagctg cgaacgttaa accccagctc gcgtaacacc cgctcaaaca cgccattctg
     9721 ctcaaaacag taaccgccac gacgggctat caccagtttc tcttccagcg attgatcatc
     9781 aagctgtatt tccctcggca gcaaaacgtc aaggttttca aacggaatgg tgcaattgtg
     9841 tttcaggtgc aatgcacgaa gcgtatcaat attgaccgca gcagctcccg accagttaat
     9901 acgggcaaaa tagtgattca gaatgggcgt catcaggcgt ttcccttagt ggttttctca
     9961 acttataacc tggaatagac gccgcaccgg ctattttgtg cgatcgcgga catgcatcac
    10021 gtttgcaaat cgccaatccc gttaatttcg ttacttattg cgttacggac tacgcttaaa
    10081 aaaaccttct tcgcgaggcg ataccatgag ccgattcatc cccatagaat tacaccatgc
    10141 cagccgtctg ttaaatcatg gcccaactgt cttgatcacc agtttcgatg agcaatccca
    10201 gcggcgtaac attatggctg cagcctggtc aatgccggtg gagtttgaac ctccacgcgt
    10261 ggcaattgtg gtagataaat cgacatggac cagggagttg attgaacgta acggtaagtt
    10321 tggcatcgtt atcccgggcg ttgcagcaac taactggacg tgggcggtgg gaagtgtgtc
    10381 ggtgcgtgaa gaagataaat ttaattgcta tggcattccg gtggtgaaag gtccggtgct
    10441 tggtttgcca cttgttgaag agaaatgcct ggcgtggatg gagtgccgcc tgctacctgc
    10501 gacttctgcg caacaaaaat acgacacgct gtttggcgag gtggtatcag cagcggcaga
    10561 cgcacgggta tttgtcgaag gccgctggca gtttgatgat gataagctca atacgttgca
    10621 tcatttaggt gctggcacgt ttgttactag cggcaagcgt gttacggcag gttaagcatt
    10681 acatgctgta accgggtttc tttatcaaag cctccatttg gggctcgatt tcggcatccc
    10741 agatagcttg ccaagattct ggttgaatct gctgtagagc aatgcttatc gagctatctt
    10801 tactgttcag atggcgaata ataacgtcgg taatatctgc agcaagtgct gctttttgtt
    10861 gttcgtccag ttcacgcgga aagcatttaa tgtcgatgtg cggcatgatt acgctccttt
    10921 tgaaagatgt cagtaaaatt aacacacatt gtgaatgtca tgtgtccggt aaagcgttta
    10981 tacgacaata atgcccaggg caagattacg aaagcccgct ccccgtaagg actgacgcca
    11041 ggtagtttct gcccatggct gcttttcgca tcttacgtct taaccctgcc ttgaatacct
    11101 tatcattcgt caaaatatta atagcaatgt gccgtatccc tgaaaataat tctgctgcat
    11161 ttcctcttct tattttgcag tcgtcttcat tcattaccac gt
//
LOCUS       DAZPAC010000130        11123 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZPAC010000130 DAZPAC010000000
VERSION     DAZPAC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.130"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            135..764
                     /locus_tag="ACHUPM_002881"
     CDS             135..764
                     /locus_tag="ACHUPM_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR0870605.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            791..2452
                     /locus_tag="ACHUPM_002882"
     CDS             791..2452
                     /locus_tag="ACHUPM_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR0870606.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            2691..2750
                     /locus_tag="ACHUPM_002883"
     CDS             2691..2750
                     /locus_tag="ACHUPM_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0870607.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3067..3126
                     /gene="ibsE"
                     /locus_tag="ACHUPM_002884"
     CDS             3067..3126
                     /gene="ibsE"
                     /locus_tag="ACHUPM_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFR0870608.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3246..4679)
                     /gene="hldE"
                     /locus_tag="ACHUPM_002885"
     CDS             complement(3246..4679)
                     /gene="hldE"
                     /locus_tag="ACHUPM_002885"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR0870609.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(4727..7567)
                     /gene="glnE"
                     /locus_tag="ACHUPM_002886"
     CDS             complement(4727..7567)
                     /gene="glnE"
                     /locus_tag="ACHUPM_002886"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR0870610.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7590..8891)
                     /gene="ygiF"
                     /locus_tag="ACHUPM_002887"
     CDS             complement(7590..8891)
                     /gene="ygiF"
                     /locus_tag="ACHUPM_002887"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR0870611.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9133..9753
                     /locus_tag="ACHUPM_002888"
     CDS             9133..9753
                     /locus_tag="ACHUPM_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR0870612.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            9817..11055
                     /gene="cca"
                     /locus_tag="ACHUPM_002889"
     CDS             9817..11055
                     /gene="cca"
                     /locus_tag="ACHUPM_002889"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR0870613.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
BASE COUNT         2683 a         3047 c         2824 g         2569 t
ORIGIN      
        1 aataaaaaac tatattaaaa aactttctct tccagaactt ctctaacatg tgttattatt
       61 aacaaagata aatgtaagca aggaaactat cccaaagtaa atattgcgtc actaaatgga
      121 cattggagtg acatatgatt ttattcgccg actataatac cccttatctt ttcgctattt
      181 cctttgtact gttgatcggc cttttagaaa ttttagcgct tatctgcggt cacatgctct
      241 ccggtgctct tgatgcacat cttgaccatt ataattccat aaccacaggt catattagcc
      301 aggcacttca ttatctcaat atcggaagat tgccagctct cgtcgttctc tgcttgctgg
      361 ctggtttttt tggtcttatt ggcatcctgt tgcagcatgc ctgcatcatg gtctggcaat
      421 caccactgtc gaacctgttc gtggttcctg tcagtttact gtttacgatt attgcggtgc
      481 attataccgg taaaatagtt gcgccctgga ttccacgcga ccatagttca gctattacag
      541 aagaagaata tattggtagc atggcattaa ttaccggtca tcaagccact tcaggtaatc
      601 cctgcgaagg aaaactcacc gatcaatttg gccaaataca ttatttacta ctggagcctg
      661 aagaaggaaa aattttcact aaaggagtca aagtgttaat tatttgccga ctttctgcaa
      721 cgcgatattt ggcggaaaac aatccctggc cacaaatatt gtaatttatt aatcaaaagg
      781 aaatttaata atggatgata ttgttaattc tgtgccctcc tggatgttta ccgcgattat
      841 tgccgtatgc attctgttta ttattggaat tattttcgcc aggctctatc gtcgcgcttc
      901 ggcagagcaa gcttttgttc gtactggttt aggtgggcaa aaagtggtga tgagcggtgg
      961 cgcaatcgtg atgccgatct ttcatgaaat aatccccatc aatatgaata ctctgaagct
     1021 ggaagtcagc cgctcaacca ttgatagcct gattacgaaa gatcgtatgc gtgtcgatgt
     1081 agtagtcgct ttctttgtac gggtaaaacc gtctgtagaa ggcatcgcca ctgcggcgca
     1141 gacgttgggg caacgcacgc tatcaccaga agacttacgt atgttggttg aagataaatt
     1201 tgtcgatgcc ctccgtgcaa cagctgcaca aatgaccatg catgagttac aggatacccg
     1261 cgagaacttc gtgcagggag tacaaaatac tgtcgcagaa gacctatcga aaaacggtct
     1321 tgagctggaa agcgtttcac ttaccaactt taaccagacc tcgaaagaac atttcaatcc
     1381 gaacaatgcc tttgacgccg aaggtttaac caaactgact caggagacgg agcgccgtcg
     1441 tcgcgaacgt aacgaagttg aacaggatgt agaagttgcg gtgcgtgaga aaaaccgtga
     1501 tgcactttcg cgcaagttgg agattgaaca acaagaagcg tttatgacgc ttgagcagga
     1561 gcagcaggtt aaaacccgta ctgccgaaca gaatgcacgt attgcggctt ttgaagctga
     1621 acgtcgtcgt gaagcagagc agacgcgaat tctggctgaa cgacaaattc aggaaacgga
     1681 gatcgagcgc gagcaggccg tccgctcaag aaaggttgag gctgaacgtg aagttcgtat
     1741 taaagagatc gaacagcagc aggtcaccga aatcgccaac cagacgaaat cgatcgctat
     1801 tgccgccaaa tcggaacaac agtcccaggc agaagcgcgt gctaaccttg cactcgcaga
     1861 agcagtaagt gcgcaacaaa acgtagaaac cactcgccag accgcagaag ccgatcgtgc
     1921 taaacaagtt gccctaatcg ctgccgcgca ggatgcagaa accaaagcgg ttgaactgac
     1981 cgtgcgggcg aaagcagaaa aagaagccgc agagatgcag gcggcggcta tcgttgagtt
     2041 agccgaagct acacgtaaaa agggtctggc ggaagcagaa gcacaacgtg cgctgaacga
     2101 tgctatcaac gtactttctg atgaacaaac cagccttaaa ttcaaactgg ccttgttgca
     2161 ggcgctacct gcggtaatag agaaatccgt tgagccgatg aagtccattg acggtatcaa
     2221 gattattcag gtcgatggtc tgaatcgtgg cagcgctgcg ggtgatgcaa acacgggtaa
     2281 tgtggggggc ggaaacctag cggaacaagc attatcagcc gctctctctt accgcacaca
     2341 ggcaccgctg attgactcct tgctcaatga aattggcgtt tcaggcggct cactggcggc
     2401 attgacttca cccttaacct caacaactcc cgtcgccgaa aacgtagaat aaattttcag
     2461 ctatgaacgt ccctggcaca aaaagccggg gattttttat atctgcgttc cgctaaaaac
     2521 tgcaaatgat caggccgttg caacgttttg cgtgaccgct cggggaaggc aaaactgcct
     2581 ctgggaaagc gttgcgcgag gtccggcgct catcaacaat cggggagtca gcaaagggct
     2641 gaaacgggaa agcccctccc gaggaagggg ccttacagaa ggaaagggtt atgatgaagc
     2701 tcgtcatcat actgattgtg ttgttactcg taagtttcgc agcttattaa cagccaatca
     2761 gaggggggag aaatcctccc tcacccttgt tcctttactt taggttgaaa aaaaatcagc
     2821 gtcaagaagg tttcattaac acatatccct ggcccaaaaa gccggggatt ttttatatct
     2881 gcgttccgct aaaaggtgca aatgctcagg acgttgcagc gttttgcgtg atcgctcggg
     2941 gaaggcaaaa ctgcttcagg gaaagcgttg cgcggggtcc ggcgctcatc aacaatcggg
     3001 gagtcagcaa ggggctgaaa cgggaaagcc cctcccgagg aaggggcctt atagaaggaa
     3061 agggttatga tgaagttcgt catcatactg gttgtgttgt tactgttaag tttcccgact
     3121 tactaacaac tgatcagagg gggaagaaat cctcccttac ccttgttcct ttactttagg
     3181 ttgaaaaaac aacagcgtca ataggcctgc ctgctacaaa gcgagatctg tgaaccgctt
     3241 tccggttagc cttttttatc ctgttggatc ttcttgatga tgttagtggt cgagcaaccg
     3301 tcttcgaagt tgagcaccag cacttcgccg ccgttggccc agacttcttt actcccggcg
     3361 atctcttctg gtttatagtc gccgcccttc accagtaaat ctggcaggat cccggcgatc
     3421 aagcgctgcg gcgtgtcttc ttcaaacgac accacccagt cgaccgcttc cagtgcgccc
     3481 agcacaatca tacgctgttc gagcgggtta acggggcggg aatccccttt cagccgtttg
     3541 gtggaggcat cgctgttcac agcgacaatc agacggtcac cctgtttgcg agcatttgcc
     3601 agataggaga cgtgcccggc gtgcaggatg tcaaagacac cgttggtcat taccaccttt
     3661 tcgccgcgtt tacgcgctgc cgctacggcc agcttcagtt cctcttcggt catcacgcca
     3721 aagccggtat ctgcgcgccc acgcactgca ttttccagct cgatcggcga aaccgtggag
     3781 gttcccagtt tgccgaccac cacgccagcc gccgcattgg caaagaagca ggcttcttcc
     3841 agcgaattac ccgctgccag cgttgccgcc aggacgccaa tcaccgtgtc gcccgcaccg
     3901 gtaacgtcat acacttcctg cgcctgggtt ggcatatgca gcggcgcttt acccggttgc
     3961 agcagcgaca taccctgttc ggaacgggtc actaacagag ccgagagttc gtaatcggca
     4021 atcagtttca tgccgcgctc aacaatctct tcttcggtct tacatttacc gacaacagct
     4081 tcaaattccg agagattcgg cgttaacagc gtagcgccgc ggtagcgctc aaaatcggta
     4141 ccttttggat caatcagcac cggaacgccc gctttacgcg ccagttggat catctgctgt
     4201 acgcttgcca gcgcaccttt ggcgtagtca gaaagcacca gcgcgccaat cgaactcagc
     4261 gcctgattaa tccgctcgtg cagcggctgc ggatcaacgc cttcgaaacc ttcttcgaaa
     4321 tccagacgga tcagctgttg gttgcgggaa agtacccgta atttggtgat ggtcggatgc
     4381 gtcggtacag aaacgaagtc gcatttgacg ttgacgtcgg ccagagattt actcagcgcg
     4441 cgcgctgcat cgtcaatgcc cgtcaacccg accaggcgtg cattagcacc gagagaagcg
     4501 atattcatcg ccacgttagc cgcgccgccc ggacgttctt cgatggtatt cactttaacc
     4561 acgggcaccg gcgcttccgg cgagatacgg ctggagggac cgtaccagta acgatccagc
     4621 attacatcac caaccaccat cactcctgca cgttcaaact ctggcagcgt tactttcatt
     4681 cctgtctcct gagagattca aaatttgcgc gcgataatac catacttcat tcttccacca
     4741 gccacttctg ccagcttgcc cgcaccagat cacgctctgc ggtgaagcaa tcccccgaca
     4801 catggcccgg caattcctgt aatgccagat gatgaagttc atcgcgcagc gtcgtgtaag
     4861 cacgggtcag cgccatcgct tcctgctctt ccataatgtc gttttgcgcc agcagctcca
     4921 gaatacgcac gttgtctgac cagcgcgtta acttcggttt ttcatgagcg tagcgcaaca
     4981 ccagatattg ggtaataaat tcgatatcgg taattccgcc ttcatcagct ttgatatcaa
     5041 agcgatcgcg atgtttattg ccgagatgag cgcgcatttt ctcgcgcatt tcccgcactt
     5101 ccgtttgcag agttctacct tcacgcggca gcgtcataat ctcgcgacgc actgcgtcaa
     5161 agtgcgcggt gagctgcgga tcgccgtaca ctacccgcgc acgcaccagc gcctgatgtt
     5221 cccacgtcca ggcctcgttt ttctgataat cggcaaatgc ttctgtggag gtaaccagca
     5281 ttcccgccgc cccggacggg cgcagccgcg catccacttc gtacaaaatg ccggaagagg
     5341 ttcgcgtgct gaacaggtgc ataatgcgtt gcgccagacg aagataaaac tgtcgtccgt
     5401 cgatttcccg ctcgccgtca gtcatcgcat ccattgggca atcatggagg aagatcaggt
     5461 caagatcgga actgtagcct aactcccagc cgcccagctt gccgtagccg accactgcaa
     5521 aaccacgccc ttcgcgatcg ttgaggtgat tcggcttacc gtagcgggca accatttgaa
     5581 cccacgcctg ctgaacgacg gcatctatca tggcttccgc cagccaggtt aagtgatcgc
     5641 tcactttcat caccggtagc gtaccagcga tatccgctgc ggcgatgcgt aacagctgcg
     5701 cctgtttgaa ctgacgcagc gcctcaagct gttgctcttc gtcatcttcc ggcacgcgca
     5761 gcaaatactg gcgcaactca tcgcggtagg catcggtcgc cgtcggctgg taaagagtgt
     5821 ttggatcgag caattcatcc agcaataatg gataacgcgc cagctggctg gcaatcatcg
     5881 gcgatgcggc acacagagaa atcaaatggt taagcgccgc agggaattca ctgagcaatt
     5941 ctaaataggt ggtgcgggta acaatcccca ccagcaaggc ggtaatgcgc gacagcgtga
     6001 cagcagcgtc ttcacgcgca cagacatcac ttagcagatg cggcatcaga tggtcgagca
     6061 cctgacgtcc tcgcggcccg atggtgcgct tatccagctc tttgcggaaa tcggaaatca
     6121 gcgttagcac ctgtttgcga tcatcctcgc taagatgcgc cagcactggc gtagtgtcat
     6181 cttcctgcaa cgcatcctgc cacagctcac gccactgttc cgacagcgac tcttcctgag
     6241 tttcactttc gtcgtcgcca atcaattcat taaacacccg gcgcacattg ttcatatgtg
     6301 cggtcagcgc cccggtcagt tgcggccagt cagcaaagtc catcgcccac gccagccgcg
     6361 cacggttaag ctcatcagaa ggaagcgtct gggtttgttc gtcgttaatg ctttgcagca
     6421 ggttttccag acgccgcagg aacagatacg ccactcgcaa ttgttcagca tcgttttcag
     6481 aaagcagatg cagcgcagca atggcgctga gcgttggcag taaagagcac gattgcagcg
     6541 acggttcgcg cccgccgcga atgagctgga atacctggac gataaattca atttcgcgaa
     6601 taccacccgc gccaagtttg atgttgtcgg tcaaaccgcg ccgacgcact tcacgggcaa
     6661 tcattccttt catgttgcgc agcgactgaa tcacgctgaa atcgatgtaa cgacggaaaa
     6721 caaacgggcg tagcatcgca cgcaactcgt tagcatagac gccttcgcta tcgcccataa
     6781 tccgcgcttt gaccatcgcg taacgctccc agtcgcgccc ctgctcctgg taataatcct
     6841 ccaacgcggc aaagctcagc accagcgggc cactttcgcc aaacggacgc agtcgcatat
     6901 ccacgcgata gacgaagcca tcctgcgttg gttgatccag cactttaatc agccgctgcc
     6961 ccatgcgggt aaaaaactgc gcgttatcca gttcccggcg tccgccctgc gtacaaccgt
     7021 gttccggcca ggcaaaaatc agatcgatat cagaggagaa attcagttcc ccgccgccca
     7081 gcttacccat gcctaagatc agcagcggtt gcgcttcgcc ctgcgcattg cacggcgttc
     7141 cccactcgcg gcagcaggcg tcatacagcc agtcacgcgc cgcaacaatc agcgtctccg
     7201 ccagataact gagctgctgc aatatgctct cttcagtaac cagtgccagc gtttgcgccc
     7261 atgcgatgcg caccataatg cgccgccgga atagccgcag ctcgcgcatt aacccggctt
     7321 cgtcactcac attactgagc gcctcctgca accatgcagc gtaatgctgc cattcatcgg
     7381 cctgcggcgg ttggctttcc agttccgtca accactctcg atgcgcaatt atgctgtcct
     7441 gcacaaaatc actgaaagta agtactgact tcgcctgtgc acttagtggt tcctcggcta
     7501 aaggctctgg cagccgctca acaacggtct gcctgtactg ctgtaacggt gaagagagcg
     7561 gcttcataag cgattttatc cttgcctgat taacgttttc cgctgtgcaa ccagaacggt
     7621 tcctgattgt ttgcctcatt acggaaatgc tcaatttcaa tgcgttgccc ggtcgcaata
     7681 gcgtgacgca gcccctgcca gttctccagc caggcatgcg cgacgacagg atcatagtaa
     7741 cccgccagca acagtattga gtcaatgtca cgcgtcaggc gtggcaactg gtcacggtag
     7801 agatcgccta acggctggca gaaaacgctt ttcagttcag cggcatggtg ggaaagatgg
     7861 atatcggcaa agcgtttgaa ggagtcgctg attttgccct gcgctttggc atctaaaaat
     7921 ggctgccatg ctttgcttac caaccattcg gtcaacgcca gcttcgccat tgccgtttcg
     7981 gtagagtaga ccgccgtcac ggcagaaacc gcagaagcaa tggtcgcttc gcattgggtc
     8041 agcaaatcac gtaagtgagt gctcgcttta cgcggcacaa taccaccgaa cagcatcagc
     8101 gtatgacgga ccaggctaat ggctgccagc acctgttctt tcgccgcatc gttaccgcgt
     8161 acccacagtt cttcatgata ctgccattgc gctaacgcca gctcgagcgc cgcttccagc
     8221 ccctgttcta catcggcttt tgccgcaaca tgcaaaatgg tggtcggttt gatttcacgc
     8281 gccggattgc cctgcgccag atgatagcca cgcgccgctt tgctcaggct gccctggcgt
     8341 aatccggttt gcgataccag ttggttcgcc agtttcagca ccgcgcgcgt gtcgccgcta
     8401 agcagttcca gttccagctc acagataggt tcagcaaatt cccccgcttt cacctccccc
     8461 tgatcgagag cgatttcaat tcggctgcca tcgaccgcca ccagccattt ttcgcgataa
     8521 aaatcggtgc tgaacagcgg ttgtacgcgg gaagcgagat cggcaggcaa ttcaccgttc
     8581 ggccagactt ccgagggaaa ctgcgccagg tcgagcgtcg gttcgctcaa cgccacgtta
     8641 tattccgggc gctggtgtaa gccgcctgtc actcttcctg caactttcat ggtcatctca
     8701 tagcgaccgt tttcgccacg aatacgtaag cccatatcgt gcctacgcag ccagttatcc
     8761 ggcgtttcgt agtaaatatt cagcaactgc acggggtcat ggtgctcgcc gcccagcgta
     8821 ttgagatggt cacgcaacgc ctcaacggca ctgtgattaa caataaactt taattcgatt
     8881 tcctgagcca tggcctttac ttatcggtta tgtcacataa gtgacgatga acggcgaact
     8941 taatgcgatc ttttttgtca gtagatagta ttttgcgcca aattgccatg caacgggcaa
     9001 tttgacgggc gtaaaagttt gaagcagtgg caagacctga cacagaggat tccgattgat
     9061 gtcgaatgct ttgcgtagcg acactgatgc cactactatc gttccacttt ccatgacaat
     9121 aacgacagcc tgatgccaaa attacgcctg atcggattaa ctttactcgc acttagcgcg
     9181 actgccgtct cacacgctga agaaacgcgc tatgtttctg acgaactgaa tacctgggtc
     9241 cgtagcggtc cgggagatca ttatcgcctc gtgggcacgg ttaacgccgg cgaggaagtg
     9301 accttattac aaactgacgc caacaccaat tatgcccagg tgaaagacag ctctggccgt
     9361 accgcctgga tcccgttgaa acaacttagc actgagccaa gcctgcgctc ccgtgtgcca
     9421 gatctggaaa atcaggtcaa aaccctgacc gataaactca ccaatatcga taacacctgg
     9481 aatcagcgca ccgcagaaat gcagcaaaaa gtggcgcaga gcgacagcgt gatcaacggg
     9541 ttaaaagaag aaaatcagaa actgaaaaac gagctgattg tcgcgcagaa aaaggtcgat
     9601 gccgccagcg tacagctgga tgacaaacag cgcaccatca tcatgcaatg gtttatgtat
     9661 ggcggtggcg tgctggggct tggcttgctg ctcggtctgg tactgccgca cctgatccca
     9721 agccgcaaac gcaaagatcg ctggatgaac taaatcgcct tcttcgttgc actgtcttat
     9781 tattaggaat tgtgtatttg acgaagggat gatggcgtga agatttatct ggtcggtggt
     9841 gctattcggg atgcattgtt agggctaccg gtcaaagaca gagattgggt ggtagtcggc
     9901 agtacgccac aggagatgct cgacgcgggc taccagcagg taggccgcga ttttcctgtg
     9961 tttctacatc cgcaaacgca tgaagagtat gcgctggcac gtaccgaacg gaaatccggt
    10021 tccggttaca ccgattttac ttgctatgcc gcaccggatg tcacgctgga agatgatctt
    10081 aagcgtcgcg atctgaccat taatgcgctg gcccaggacg ataacggcga gattatcgac
    10141 ccgtacaacg gtctgggcga tctgcaaaat cgtctgttgc gccatgtttc ccccgctttt
    10201 ggcgaagatc cgttacgcgt actgcgcgtg gcgcgttttg ctgcgcgtta tgcccacctc
    10261 ggttttcgta ttgccgatga aactctgacg ctgatgcgcg agatgaccca tgcgggtgaa
    10321 ctggaacacc tgacgcctga acgggtatgg aaagagacgg aaagcgccct caccacccgc
    10381 aatccacagg tgttcttcca ggtactgcgc gattgcggcg cactgcgcgt tttattcccg
    10441 gaaattgacg cactgtttgg cgttccggcc cctgccaggt ggcatccgga aatcgacacg
    10501 ggtattcata ccttaatgac gctctcaatg gcggcgatgc tgagtccgca ggtcgatgtc
    10561 cgtttcgcaa ctttatgcca cgatctcggt aaaggactga cgccgccaga actttggccg
    10621 cgtcatcatg gtcacggtcc ggcgggtgtg aaattagtgg aacaactctg ccagcgtctg
    10681 cgcgtgccaa atgaaattcg cgatttagcc agactggtgg ctgagtttca cgatctcatc
    10741 cacaccttcc caatgctgaa cccaaaaact atcgtcaaat tgtttgattc catcgacgcc
    10801 tggcgtaaac cgcagcgtgt cgagcaactg gcgctgacca gcgaggctga cgtacgcggc
    10861 agaaccggtt ttgaatcagc ggactacccg caaggccgtt ggttgcgcga agcctgggaa
    10921 gtggcgcagt cagtgccgac aaaagcggtc gttgaagcgg gatttaaagg tgtggagatt
    10981 cgcgaggagc tgacccgacg gcggattgcg gcggtagcca gttggaagga acaacgttgc
    11041 ccaaagcctg actgagtata aaactaaacg ttaacacgaa ccatcccctc gccccttcgg
    11101 ggagagggtt agggtgaggg gaa
//
LOCUS       DAZPAC010000131        11024 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZPAC010000131 DAZPAC010000000
VERSION     DAZPAC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11024
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.131"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..208
                     /locus_tag="ACHUPM_002890"
     CDS             <1..208
                     /locus_tag="ACHUPM_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR0870614.1"
                     /translation="IVPLTVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFA
                     QTTKGVLETGIYRGVVSIYLSQDI"
     gene            300..2984
                     /locus_tag="ACHUPM_002891"
     CDS             300..2984
                     /locus_tag="ACHUPM_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR0870615.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2981..4069
                     /locus_tag="ACHUPM_002892"
     CDS             2981..4069
                     /locus_tag="ACHUPM_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR0870616.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="ACHUPM_002893"
                     /pseudo
     CDS             complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="ACHUPM_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5931..7343
                     /gene="uxaC"
                     /locus_tag="ACHUPM_002894"
                     /pseudo
     CDS             5931..7343
                     /gene="uxaC"
                     /locus_tag="ACHUPM_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
     gene            7358..8845
                     /gene="uxaA"
                     /locus_tag="ACHUPM_002895"
     CDS             7358..8845
                     /gene="uxaA"
                     /locus_tag="ACHUPM_002895"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR0870617.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8928..9479
                     /locus_tag="ACHUPM_002896"
     CDS             8928..9479
                     /locus_tag="ACHUPM_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR0870618.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9484..10728)
                     /gene="sstT"
                     /locus_tag="ACHUPM_002897"
     CDS             complement(9484..10728)
                     /gene="sstT"
                     /locus_tag="ACHUPM_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR0870619.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2915 a         2672 c         2857 g         2580 t
ORIGIN      
        1 gattgttccg ctgacagtaa cctggggcgg tgaagaaatt aaagctgatg cggcaaccac
       61 ttttaccgca accaaaatct ttgcttccga tgcgttaact aacggttctc tggctaaaaa
      121 cctgatgttt gctcaaacca ccaaaggtgt tctggagaca ggcatctacc gtggtgtagt
      181 aagcatttat ctgtctcagg atatctaatc cagataacag aattaacacg taacaaaggg
      241 aggggtagcc ctcccttatt ttaactgttt tagtatttgt tatggatttc aggattgcca
      301 tggataaaaa attactggct cttttgatcc tggcgagtct cagcccggca gaggcgacat
      361 taaccgaaat tcccgcaggg tttgaggtta ttgctcaggg acagcaggag tatatcgagg
      421 tttatttttc agggaaaagt ctcggtaaat attatgcaat ggttaatctt gataccgtaa
      481 catttcttga tccagcaagt ctatataaca agctggaact ggatgtagac gatcagaaaa
      541 tcgcgcatat agtgaaagaa aaattatcgc agccgctagc tcaccacggt gaattggctt
      601 gcggttatgt acgtactgac tcagggtgtg gttttctgaa taccgatacg ctggaaataa
      661 tctataatga tgaagaaagt tcggcaacgt tgtttattaa tccgcaatgg aattcagctt
      721 tcgatgcgaa gtcattatat ttaaatccag acaaaaatac tgttaatgct tttatacatc
      781 agcaagacat caatgttctg gcacaggatg attaccaatc gttgtctatt cagggaaacg
      841 gtgcgctggg aataacagaa aatagttata ttggtgcaca ctggaatttc aacggttatg
      901 atgcagatga tgtcagtgac agtaatgctg acgtcagcga tctctattat cgttatgatt
      961 ttttacgtcg ttattatgtg caggcgggtc gcatggacaa ccgcacacta tttaatgcac
     1021 aaggcgggaa ctttaccttt aactttctgc cactcggtgc aattgatggg atgcgtatcg
     1081 ggtcgacact gagctattta aaccaggcgc aaagccagca aggaacccca gtaatggttc
     1141 tgctttcgcg caattctcgt gttgacgctt atcgtaatga gcaacttttg ggatcgtttt
     1201 atctcaatag tggttcgcaa tttattgata ccagttcctt tccgccaggc agctacagcg
     1261 tggcattaaa agtctacgaa aataaccaac tcacccgcac cgagcttgtg ccgtttacca
     1321 aaaccggcgg tctgactgac ggaaatgcgc aatggttctt acaggcaggt aaaactacat
     1381 cacaggtttc tgatgatgaa agttcagctt atcagctagg agtacgcctg ccattacatc
     1441 cgcaatatga gctctacgca gggctggcga atgccgatga tgtgagtgct ttcgagttag
     1501 gtaataactg gacggcagat ttaggcgggg tggggaatct tgcaatcagc gccagcgtgt
     1561 tccgtaacga tgacggcggc aaaggtgata tgcaacaggc caactggagt aatccgggat
     1621 ggccgacgtt gggcttttat cggaccaact ctgacggcga tgcttgtaca accgacagca
     1681 gagagagcta taacgcctta agctgttatg aaagtatttc cgcgacggtt tcacagaatt
     1741 ttgtcggctg gaatatgatg ctgggttata cccgcacaca aaataacact gatgatagtt
     1801 tgcgttggga taaacagcag agctttgaaa ataactatct tcgccagaca acagcgcaaa
     1861 gtatctctga aactgtacaa cttagcgctt cccgcgcttt tgtgatgcgt gactggattt
     1921 tgagtacgtc agtcggtgtt ttccatcgta atgacaacgg tggcgataac gacgacaacg
     1981 gcttgtactt atcgttttcg ttatctgaca cgccaacgat ggatagcaat aacaacagcc
     2041 attcaacaaa tgtgtctacg gattatcgtt atagcgaaca ggatggcgat caaacgtcat
     2101 ggcagttatc gcataccttt tataacgatt cattcagcca taaagaactt ggcgtaaccg
     2161 tcggaggcct gaacaccgat accataaaca gcgcggttaa cgggcgttgg gatgggcaat
     2221 acggaaatgt ctacgctacc gtatctgata gttatgaccg taagaatcat gatcatcttt
     2281 cggcctttac gggcacttat agctctacgc tggctgtcag ttgctatggc gttaatttgg
     2341 gagccagtgg tacagacgat ttgctgggtg cggtactggt ggatgtgaaa ggcttctctg
     2401 aacaggatga agagagtcag gatctgcaac tcgaagcgcg ggtggcaggc agccgaactt
     2461 tgcagcttgg tcaaagtgac agtgtgttgt tcccttatcc tggatttcag tctggttttg
     2521 ttgaggttaa cgacagtagc cagggcaatc agcaggggac aacaaacatc attaacggtg
     2581 cagggaatcg tgaattaatg ttgttgcctg gcaagctgcg ctatcgcgaa gtgtctgcca
     2641 gctttaacta caactatatc ggtcgtttat tactgccggc agcggtgaaa aaattcccga
     2701 ttgtggggct gaacagcgcc atgttactgg tagctgaaga tggaggattt acacttgaaa
     2761 ttaacggcag cgaaaaagag ctctatctgc tttccgggca gcaattcctt aagtgtccac
     2821 tgagtgttgt aaagaaacgc gccagcattc gttacagcgg agatgttact tgtagtgtgg
     2881 tgacttattc acaattaccg gagtcgattc aggttcaggc acagttgaaa cagcctaaat
     2941 tacgtggaaa cgttcagacg gcgcaaaggg aggttgcacc atgagaaatc gattgattgc
     3001 ggcgatattg ggcttgtttg gcacgctcac tggcgttcag gcagctcctg acgtgaccag
     3061 tgaaattacg tatgatttgg catctggtag agcggattat tacttctgga aggatgaagc
     3121 gtcggcagga aataatggat atatgtggta tgaatgttcg tatcctgacc tccaacaaac
     3181 ctgtacagct aatggaaata tatcgacagt acaaatctat ttaactgaac aacgcagtgg
     3241 gatgcgttgg ccggtaaaac tcaaaggatt taaaacagcc attgtaagta gcgatgaagc
     3301 gccgccagga tgcaaggggg gcaaagggct tcagacgaat cttaaggatt ctaatagatc
     3361 ttcatgtaca gaagatggtc aacattatta tatatacgat acaaagtttc ttacgctcta
     3421 ccttgagcag acagagatga agaatttgcc gattggtggc gtctggaagg ggaaagttaa
     3481 attacattcg aacagcccgg cccaggacta tttcgcaaat attaccctga atacgctcga
     3541 ccccaaccat attgacgtgt tcttcccgga gttcgcccac gccacgccaa gggtgcagtt
     3601 agacttgcat ccaacaggaa gcgttaacgg cagcaactac gcgcaagatc tgaccatgtt
     3661 ggacatgtgc ctgtacgatg gttttaacgg taatgccatc agttatgaaa tcatgctcaa
     3721 agatgaaggg cgaccagctg cagggcgcag agacggtgac ttctctatct atcgtcaagg
     3781 agggaccacc accgacgagg gagaacgcat tgattaccgg gtcaaaatgt acaacccgga
     3841 aaccggtggg caaattgatg tgcgcaataa tgaaaatatg gtctggaaca gcattaacct
     3901 gaaacgtgtg cgtccggtgg tactgcccgg tattcgctat gccgtgatgt gtgtgccgac
     3961 acccttgact ctggcagtcg ataaattcag cgtgatggat aaacaggccg ggtactacat
     4021 gggcaaattg tcagtaatct ttacgccttc cttgccaacc atcaattgac cccgcaggcc
     4081 agaaaaaact aaacaacgaa gccgcctgat aaggcggctt cgtcattaca acttacgtta
     4141 aacgttaaac gttaatgttg cggtgcggga tcgttatgtg tttcctgcgc cacctcaatc
     4201 gccggtttgt tctgcaatac ggtccagata accagcgcac ccagcaggtc aaatactgcc
     4261 agaactgcga acagcgggct gaagccgatg gtgtcagcca gtgcaccgac aaccagcgca
     4321 aacagcgtac ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg
     4381 ttacgaccga acacgtcaga agagagcgta atcagcgcgc cagacagtgc ctggtgggca
     4441 aaaccaccga tacacagcag cataattgcg acatacgggt tggtgaacag accgatcata
     4501 cccgggccaa tcatcagcac agcacccagc gttacgacca tcttacggga aacgatcagg
     4561 ttcacaccaa accaacgctg gaacagcggc ggcaggtaac caccgaggat acaaccgagg
     4621 tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa
     4681 actttaaaca tgaacagcgg gatccacgcg ttaaaagtac cccaggccgg ttctgccagg
     4741 aaacgcggca gtgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt
     4801 ttcttcgccg tgctcacctg gtgctgggct tcctgaccat taataatata gtcgcgttct
     4861 tcatcggtca gatgcttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc
     4921 cagataaagc tcaatgcacc ggagatgata aatgccatct gccagctgtg catcacgatt
     4981 gccataccac cagcggcggc gcaatcatcg caccaatcga agaacctacg ttaaagtagc
     5041 ctactgcgat ggaacgctct ttcgccggga accattcgga gctggctttc agacccgccg
     5101 ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc aacagccagg ccaccccagc
     5161 tacctgccag cgcggttgca ccacagaaca cggcccacag cacagcaaac attgcataac
     5221 cgattttcgt acccagcaca tccagtacat aacctgctac cggttgcatg accgtataag
     5281 cagcagaata ggctgcgatg atataggaat actgttgggt ggagatgttt aactcttcca
     5341 tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc acggtgccga
     5401 gcgtcaccag tgcgatcata taccaacgta accctttaat tttacgcatg taaaacctca
     5461 tcgttttgtt atatccgcag gaagcgcggc catcatcccg ccagaggctg aagatgttag
     5521 ttacgaccgg gtaaccgaaa aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt
     5581 gctaattcgg cttccgtacc ggttaacccg attcatctaa caaatgaatg aatcggcaat
     5641 ttgcattccg tcgacttatg atttgcgacg gcagaaagat aacttgtcat acaactttaa
     5701 aaggtgagag ccatcacaaa tgtgcgaata tttgtaggga cattacctga cgacagcgag
     5761 gccagtactg gcgcggcctg cagcgagatt tatcactttg agagtaattt tttaactacg
     5821 tttattgatc taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt
     5881 cagcatcgca ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt
     5941 ttatgactga agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg
     6001 caaaagacca gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag
     6061 actatcgttt taaaaacctg tatgacatct ggctgaaagg cgatcactac aaatggcgcg
     6121 ctatgcgtac caacggtgtg gccgagcgtc tgtgtaccgg tgatgcatct gaccgtgaaa
     6181 aatttgacgc ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga
     6241 cgcaccttga actgcgtcgt ccgtttggta tcactggcaa attgctttct ccgtcaactg
     6301 ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg
     6361 gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc
     6421 tggagcatca cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct
     6481 ggcgtccgga caaagccttc aacatcgaac aggcgacctt taacgactac atggcgaagc
     6541 tgggcgaagt ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca
     6601 aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg
     6661 tgatgtttgc tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg
     6721 gcgaaaccct gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg
     6781 gtgccgaata tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata
     6841 acaacctgcg tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc
     6901 gtccgatggc ggaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc
     6961 cgaaaaccat tctctactgc ctgaacccgc gcgataacga agtgctgggc accatgatcg
     7021 gtaacttcta gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta
     7081 acgatcagaa agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtctgctga
     7141 gccgctttgt cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat
     7201 acttccgccg cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg
     7261 cggacatcaa cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact
     7321 acttcgccat tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc
     7381 gctggataac gtcgcggtcg ctttagcaga tttggctgaa ggcacagaag tcagtgtcga
     7441 taaccagact gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga
     7501 tatcgcaaaa ggggccaacg tcatcaaata tggcctgccg attggttatg cattggcgga
     7561 tattgcggcg ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga
     7621 tcagtatcgc tatcaacctg attttcagga tctgcctgcg caagcggcag atcgtgaagt
     7681 gcagatctat cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc
     7741 aaccgtgggc tgtgtcaacg gcatcgcgcg gcaaatccag aaccgtttcc tgaaagagac
     7801 caacaacgcc gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca
     7861 actgggcgac gatcacatca acatccgcac catgctgcaa aacatggtgc gccacccgaa
     7921 cgcgggcgca gtgctggtga ttggtctggg ctgtgaaaac aaccaggttg ccgcattccg
     7981 tgaaacgttg ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga
     8041 tgatgagatc gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga
     8101 taaacgcgag ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga
     8161 cggtctttct ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc
     8221 taacggcggt actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct
     8281 gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt
     8341 taaacagtac tttattgccc atgatcagcc gatctacgaa aacccatcac cggggaacaa
     8401 agcgggcggt atcaccacgc tggaagacaa atcacttggc tgtacccaga aagcgggttc
     8461 cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg aaaacgcctg ggctgaactt
     8521 gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca
     8581 tatggtgctg ttcagtactg gtcgcggcac gccatacggt ggatttgtgc cgacggtgaa
     8641 aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac tggatcgact ttgacgcggg
     8701 tcagctgatc cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt
     8761 tgagtttgcc aacggtaagc aaacttgcaa cgagcgtaac gacttccgcg aactggcgat
     8821 cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc
     8881 ataatgtgaa gcgccgtttg cttttccata cctgataagg atttatcatg accgcgtatt
     8941 ggctggccca gggcgtgggt gtcatcgcct ttctgattgg tatcacaaca tttttcaatc
     9001 gtgacgaacg tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt
     9061 tttttcttct gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat
     9121 tgattacctt acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg
     9181 gaattggcct cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg
     9241 tcagtacctg ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt
     9301 caacgtgttg ctgggtgatt cacaacttct gggcggggtc gataggcggc acaatgattg
     9361 agggtagttt tctgcttatg aatggcttga atatcattcg tttctggcgg atgcagaaaa
     9421 ggggaattga tccgtttaaa gtagagaaaa cccctcccgc cgtagacgaa aggggttaaa
     9481 caattaatta cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc
     9541 agtgaacagc acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc
     9601 gatgataaag ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt
     9661 acaggccagc gggatcagca gcagagaacc ccccgccacg ccggatgcgc cacaggcaca
     9721 cagagaagcc accacgctca acagcagcgc cgtgggcaga tcgaccggga tacccagcgt
     9781 attaaccgca gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt
     9841 ggcacccagc ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag
     9901 tgccatattc accggaatgt tcgccgcaga gctgcgggtg aagaaggcat acacgccgct
     9961 ttcgcgcagg cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag
    10021 caatgggtta accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg
    10081 cgcgtagccc cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagaccaaa
    10141 aataccaatc ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg cattcgacat
    10201 atcgttgacc aggtttttgg tggtctcgtt accgtgacgc agtgcgaaac cgaggccaat
    10261 tgcccacacc aggatcccga tgtagttacc tttcagcagc gcgtcgatgg ggttggaaac
    10321 catgctcatt accagcccgc gcatcacttc tacaatgcct gacggtggcg aaatatcacc
    10381 cgcgctactg gataagtgca gggtagaagg gaaggcgaag ctgaaaacga ctgcggccag
    10441 agcagcagag aaggtaccca gcagatagag gaacaaaata gggcggatat tggttttctg
    10501 cccatgctgg tggttagcaa tagatgccat caccagcatc aacaccagga tgggggcaac
    10561 ggctttcagt gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt
    10621 tgatatccat gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct
    10681 gccatgagcc agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt
    10741 tcattgtgtt atcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa
    10801 agtgtttgca aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat
    10861 tttaaagaga agtcatattt tgaaacttaa tggttacatt tgtttgacat aaacagaggg
    10921 aaggactggc tactaaggct gattagataa catatgggca aaactatttg gaatatgaga
    10981 acggttgcgc tcctttaatg tgctatgcca tataaagtca ctgg
//
LOCUS       DAZPAC010000132        11000 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZPAC010000132 DAZPAC010000000
VERSION     DAZPAC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.132"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..277)
                     /locus_tag="ACHUPM_002898"
     CDS             complement(<1..277)
                     /locus_tag="ACHUPM_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0870620.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRS"
     gene            complement(444..1184)
                     /locus_tag="ACHUPM_002899"
     CDS             complement(444..1184)
                     /locus_tag="ACHUPM_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR0870621.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1277..1912)
                     /gene="argO"
                     /locus_tag="ACHUPM_002900"
     CDS             complement(1277..1912)
                     /gene="argO"
                     /locus_tag="ACHUPM_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR0870622.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="ACHUPM_002901"
     CDS             complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="ACHUPM_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR0870623.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            3107..3205
                     /locus_tag="ACHUPM_002902"
                     /pseudo
     CDS             3107..3205
                     /locus_tag="ACHUPM_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3269..4348)
                     /gene="fbaA"
                     /locus_tag="ACHUPM_002903"
     CDS             complement(3269..4348)
                     /gene="fbaA"
                     /locus_tag="ACHUPM_002903"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR0870624.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4563..5726)
                     /gene="pgk"
                     /locus_tag="ACHUPM_002904"
     CDS             complement(4563..5726)
                     /gene="pgk"
                     /locus_tag="ACHUPM_002904"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR0870625.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5776..6795)
                     /gene="epd"
                     /locus_tag="ACHUPM_002905"
     CDS             complement(5776..6795)
                     /gene="epd"
                     /locus_tag="ACHUPM_002905"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR0870626.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7167..7598
                     /locus_tag="ACHUPM_002906"
     CDS             7167..7598
                     /locus_tag="ACHUPM_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFR0870627.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7621..8199
                     /locus_tag="ACHUPM_002907"
     CDS             7621..8199
                     /locus_tag="ACHUPM_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR0870628.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8200..8907
                     /locus_tag="ACHUPM_002908"
     CDS             8200..8907
                     /locus_tag="ACHUPM_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFR0870629.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RQAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8895..9572
                     /locus_tag="ACHUPM_002909"
     CDS             8895..9572
                     /locus_tag="ACHUPM_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0870630.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9566..10243
                     /locus_tag="ACHUPM_002910"
     CDS             9566..10243
                     /locus_tag="ACHUPM_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0870631.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10215..10928)
                     /locus_tag="ACHUPM_002911"
     CDS             complement(10215..10928)
                     /locus_tag="ACHUPM_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR0870632.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10925..>11000)
                     /locus_tag="ACHUPM_002912"
     CDS             complement(10925..>11000)
                     /locus_tag="ACHUPM_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0870633.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2627 a         2918 c         2740 g         2715 t
ORIGIN      
        1 gtgaacgacg ccctgagtta tggatttcat tatcaatcgc agaaagtcgt tgataaaccg
       61 ggaacagttt ttgatacagt tcctgaccgg ctttattgag gctgatatta ttatctttgc
      121 gggtaaatag cgtatagcca atttgttctt ccagcgcggc aatgctttta ccaaatggcg
      181 aagccgtcat atggattttt tctgccgccc gggcaatatt attggtttgc gctaataaaa
      241 tgaaattgcg catctttttc gaaataaaaa tgtccatccc ccctccccgc atccttgtcc
      301 gcttaaccgt ttcagtcagc ctatccttga tgaaaccgcg agcaaagata ggtgattacg
      361 tcatggtttt acagaaaatt acagaaaaag gaggcaatat cgggtaaagg cattagcccg
      421 acgaatacgt cgggctacaa gtattattgt gctgcaggtg ttttagcggg ttgttgatcc
      481 acaggttcta actggaagac cacatcgacc tgatcatcaa actgaatagc ggcctgctcg
      541 taagtttcct gggcagacac cggcgcggca tcggctttca tcatccgcac cattgggctg
      601 ggctgatagt tggaaacatg gtagcgcacg ctatataccg gccccagttt acgatgaaag
      661 ccgttcgcca gttcctgcgc ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg
      721 tctttatagg catccggctg cgccacgccc agcgacacag aacgaatttc gttaagaccc
      781 gccttcagcg cgccatccag caaggaattc agtttgtcta actgacggag cgtgacttcc
      841 accgttctca cagcgcggta gcctttcagg atacttttac catcctgata atcataatct
      901 ggctgggtgc gtaagttcgc tgagctgata tcttttttcg cgatctgatt gagttcaagg
      961 aaggaaatgt attgtgcgac gcgctcatct gcctgtttct tggctgtagc ggcatccttc
     1021 gcggccacgt taacttcaat cgcaagagtg gcaatgtctg gcaccgcatc cacacttgcc
     1081 gtaccggagg tgacaatatg cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg
     1141 ctaataccca ttaatgccgc cagggcgata actttgaact tcacgttagt ctctccgtgt
     1201 taattgactt gcccaaaatt caggacctct ggaggcaagc ttagcgcctc tgttttattt
     1261 ttccatcaga tagtgcctaa ctgaacaagg cttgtgcatg agcaataccg tctctcgcca
     1321 gctgcaaggc tataaaccac atgacacatc ccactaccag attgataatg cgctgtgatt
     1381 ttgccgtgcg caggcgcggt gccagccagg ctgcgagaat agccagacca aagaaccaca
     1441 ggaaagaggc gctaattgtc ccgagcgcaa accaacgttt tggttcgacg tcaagctgcc
     1501 cgccaaggct gcccagtaca acaaaagtat ccaggtaaac atgcggattc agccaggtca
     1561 ctgccagcat ggtggcgata attttccatc tgccttgctt caggacttcg gcactagcta
     1621 actcaatatt actactcatc gctgttttaa aagcgccaaa accataccac agcaagaagg
     1681 ctacgccgcc ccaggtgacc agcgccagca accacggcga ctgcatcaat aacgcgctgc
     1741 caccaaaaat cccggcgcaa atcaggacca aatcgctgat agcacaaagt aaggcaatca
     1801 taatgtggta ctgacgacga atgccctgat tcatcacaaa agcattttgt ggaccgagcg
     1861 gcagaatcat agctgcccca agagcaatac cttgaaagta ataagaaaac acgttggata
     1921 ttccgaatta agtatctgat gcagagacta tatcgcggga ttgttattag cggaaattga
     1981 taatattaat tggcaatcag aataactaat aagagagagg ccagagagta ttatctggcc
     2041 tcagcgttga ttacgcagct ttgtcttctt tcacccgctt aaagttcaca tccatttgcg
     2101 ggtacgggaa gctgataccg gcggcatcaa attcacgttt aatacgctcc agcacatccc
     2161 agtacacgtt ttgcagatcg ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg
     2221 atgcaccaag ttcgttcaga cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag
     2281 actggataat attggtcagg atctgcttaa cctgatcgat atcggaatca tacgccacgc
     2341 caataataaa ttcgttacgg cgaactggct cgcgagagaa gttaataata tttccggcaa
     2401 taattttacc gttcggaata acgataattt taccgtctgc agtacgcatg gtggtggaga
     2461 aaatctgcac actcagcaca gtaccggcta cgccgccgag atcaacatat tctccggcac
     2521 ggaacgggcg gaacatgaca agtaacacgc cagcggccag gttagaaagt gacccctgca
     2581 aagccagacc aacagctaag cctgcggcac cgagtacagc aatgactgac gctgtttgta
     2641 cacccacgcg tcccagggca gcgattagcg taaaggcgat aataccgtaa cggactaatg
     2701 cagaaagaaa atcagcaaca gtggcatcga ttttacggga gatcatcagg cgattcaccg
     2761 cgttggaaat catccgcgcg ataatcaaac caacgatgat gatcgcgagt gccgccacga
     2821 tgtttactgc ataacttagc agcagcgcct ggttagctac cagccagctt cccgcgccgt
     2881 ttatgctatc gacaacattc aaatcttcca ttcaatattc cttttcaact gactcaaaat
     2941 ggagaaatac actccgccgc cttatgacgg gcagtctgac aagggtaaac aataaagcat
     3001 aattttgcca aatagatcac agatttctca tctgaatcac cttgcttttg gcgaaaaaat
     3061 ccccgcagag cggggctcag acagatatca atgtgcgctt tgtcatgccg gatgcggcgt
     3121 gaacgcctta tccggcctac aaaaacctgc aaattcaata tattgcagga tacgtgtagg
     3181 cctgataagc gtagcgcatc aggcaatttt gcttttggcg aaaaaaagac ccgcagagcg
     3241 ggccttgaga taagcagaaa ggaatatctt acagaacgtc gatcgcgttc agttcctgga
     3301 atgctttttc cagacgagcg atcatcgaag tctgaccggc acgcagccat acgcgcggat
     3361 cgtagtattt cttgttcggc tgatcttcgc ctttcgggtt acccaactgc ccctgcagat
     3421 aagcttcgtt cgctttgtag tagttcagaa cgccttccca ggttgcccat tgggtatcgg
     3481 tatcgatgtt catttttact acgccgtagc ttacggagtc tttgatttcc tgagcagtag
     3541 aaccggaacc accgtggaat acgaagttca ggctgttgtg cggcaggttg tgtttcttgg
     3601 aaacatattc ctgagaatca cgcaggatgg tcggagtcag aaccacgtta cccggcttgt
     3661 aaacaccgtg tacgttaccg aaggacgcag cgatggtgaa acgcgggctg attttgctca
     3721 gttcggtgta tgcgtaatca acgtcttccg gctgggtgta cagtgcagaa gcgtccatgt
     3781 ggctgttgtc cacgccgtct tcttcaccac cggtgcaacc cagttcgatt tccagagtca
     3841 tgccgatttt ggacatgcgc tccaggtatt tagagcagat ttcgatgttc tcttgcagag
     3901 attcttcaga caggtcgatc atgtgagaag agaacagcgg cttaccggta gctgcgaagt
     3961 gtttttcacc cgcgtccagc agaccgtcga tccacggcag cagtttcttc gcgcagtggt
     4021 cagtgtgcag gataaccgga acaccataat gttcagccat ctggtgaacg tgatgcgcac
     4081 cagagatcgc gcccaggata gcagcaccct gcggaacgtc agatttcacg cctttaccag
     4141 cgataaagga agcaccaccg ttggagaact gaacgataac cggcgcttta actttagcag
     4201 cggtttccag tacggcgttg atggagtcag taccgacgca gtttactgct ggcagtgcga
     4261 agttgttttc ttttgctacc tggaaaactt tctgtacgtc atcaccagtg attacgccag
     4321 gttttacgaa atcaaaaatc ttagacatgt ctcttgtcct gtatcgtcgg gccttatact
     4381 catcatactt caagttgcat gtgctgcgtc tgcgttcgct caccccagtc acttacttat
     4441 gtaagctcct ggggattcac tctcttgtcg ccttcctgca actcgaatta tttagagtat
     4501 gaaaaaatgc gtgctctaaa agcgcgctga aacaagggca ggtttccctg ccctgtgatt
     4561 ttttacttct tagcgcgctc ttcgagcatc gctactgcag gcagtacttt accttccacg
     4621 aattcgagga atgcgccgcc gccagtggag atgtaggaga ttttgtcagc aatgccgaac
     4681 aggtcgattg ctgccagagt gtcgccgccg ccagcgatgg agaacgcttc gctgtctgcg
     4741 atagcgttag ccacgatttc agtacctttg cggaagttcg ggaattcgaa cacgccaacc
     4801 ggaccgttcc acagaatggt tttcgcattc ttcaggattt cagccagttc ctgagcggaa
     4861 gcatcaccga tatccaggat ctgctcgtca gctttcacat cgttaacaga tttcagggta
     4921 gccgatgcag tttcagagaa ctcggttgct acgcgaacat cagacggaac cgggatgttg
     4981 caggtggtca gcagacgttt agcttcgtca accaggtcag cttcgtacag ggatttaccc
     5041 acatcgtggc cttgtgccgc gataaaggtg ttagcgatac caccaccaac aatcagctgg
     5101 tcagcgattt tagacaggga gtccagaacg gtcagtttgg tagatacttt agaaccacca
     5161 acgatagcca ccatcgggcg agcaggttct ttcagtgctt tacccagcgc gtccagttca
     5221 gctgccaaca gcgggcctgc gcacgcaaca tcagcgaatt taccgatacc gtgagtagaa
     5281 gcctgcgcgc ggtgagcagt accgaatgcg tccattacga acacgtcaca cagtgcagcg
     5341 tattttttgg acagggtttc gtcgtctttc ttctcgcctt tgttgaagcg aacgttttcc
     5401 agaacaacca gttcaccttc agcaacgtca acgccgtcga ggtaatcttt aaccagacga
     5461 accgggttag acagtttgtc tttcaggtag ttaacaaccg gcagcagaga gaattcttcg
     5521 ttgtactcgc cttcggtagg acgacccagg tgggaagtta ccatcacttt cgcgccttgt
     5581 ttcagggcca gttcgatggt cggcagagaa gcacggatac gcgcgtcgct ggttactttc
     5641 ccgtctttta ctggtacgtt cagatccgca cggataaata cacgtttccc agcaagatcc
     5701 agatcggtca tcttaattac agacatggtg aatcctctcg ttgattctaa aagttttgca
     5761 gacgctgctt gcgtcttacc tgaaagcaac agtagccata gctaacgtcg tgtcgagcat
     5821 tcggttagca aagccccatt cgttatcgca ccagaccaac gttttgatca ggtgtgcgcc
     5881 actgacccgg gtttgggtgc catcgacaat ggcactgtgc ggatcgtggt taaaatctac
     5941 agagaccaac ggcaattccg tatagtcaac tataccatga aatgcacctt gtgctgcttt
     6001 ttgcagcaac aggttgactt cattggcttt tacaggtttc ttcaccgtca cgcttaaatc
     6061 gattgccgtc acatttatgg ttggcacacg taccgcaatc gcttcaaagc gatcgttaaa
     6121 ttgcggaaaa aatcgtgtga taccggcggc cagtttagta tcgaccggaa tgatcgactg
     6181 gctggctgcc cgggtgcgac gcaggtcagg atggtatgca tcaataacct gttgatcgtg
     6241 catggcggag tgaattgtgg tcacagtgcc ggactcaata ccgtacgcat catctaacaa
     6301 tttgatgacg ggaattatgc aattcgtggt acaggaagcg ttagaaacga tgcggtgttc
     6361 cgcacgaagt tgatcctgat tgacgccgta aacaacggtc gcgtcgagat cgttactgcc
     6421 aggatgtgaa aagagcactt ttttggcccc ggcggcaata tgcgcttcgc catgctcgcg
     6481 ggagccatat acgccggtgc agtcgaggac tacatcaacg ccaagttcac gccaggggag
     6541 cgattgcagt gaacgttcat gcaatacgcg gatggcgtca tcaccaacaa aaagttgatc
     6601 gcgttcctgt cgtacttccc atgcaaaacg gccatggctg gtgtcatatt tcaacaaatg
     6661 cgccatgccc gcagcatccg ccagttcgtt gattgccacc acggtaattt ccgcccggcg
     6721 tccggattca tacaaagcac gaaccacatt acgcccgatg cgaccgaagc catttatcgc
     6781 tacgcgtacg gtcatagatc tcctgcaagg ttttccctga gcaaatttgc cagacagagt
     6841 aatccagcaa atcgtccggg gaaaccttac ctgtcgcaaa ctgcgactga ttggttaatt
     6901 gtcgaacatt taatcgactg aaacgcttca gctaggataa gcgaaacgtg gaataaaagg
     6961 aatgattgtc cagtcaaaga aaacaattat ctgacttacg tcacgttttc agccccgccc
     7021 ctgtggaatt tattccactt tgcagaatca cgaataagcg tagcaaaatc ttttcgttgt
     7081 gccgtcacaa accctgactt atatcagaat atgactgatg cagcgtcgat attatccgca
     7141 atacgtccga gtttcggagt actccaatga tgacattgcc ctacccatat tcttcccccg
     7201 cacgctttta tgcactgcgc ctcctccccg ggcaggaagt tttttcccag ctacacgctt
     7261 ttgcacagca gcatcaactt catgccgcct ggattgcagg ctgtaccggt agcctgactg
     7321 acgtcgcctt gcgctatgcc gggcaagaag gcaccacgct ccttaacggt acattcgaag
     7381 tgatctcact aaacggcacg ctggaacaga gtggtgagca tcttcatctc tgcgtttccg
     7441 atccacacgg cacgatgctt ggcggtcata tgatgcccgg ctgtaccgtg cggaccacgc
     7501 ttgaactggt gatcggtagc cttgaggaac tggcgtttag ccgccaacct tgcgcgcttt
     7561 ctggttacga cgagttgcat atttcccccg ttaaataact actttcaaag aggttttctt
     7621 atggcgcgta gccacttttc cagtcaggca ctggtgttga tcgttatatc tatcgccatc
     7681 aatatgatcg gcgggcaact ggccagcatg gtcaaattac ctatatttct cgactccatc
     7741 ggcacactta ttagcgcggt actgcttggt ccggtgatcg gaatgctgac cggcttactg
     7801 accaatttac tctggggatt gctgaccgac ccgatcgccg ccgcatttgc acccgtcgcg
     7861 atggtcatcg gcctggtcgc cggttggctg gcgcgagcgg gttggtttcg caccctgcca
     7921 aaagtcgttg tcagcggcgt gattattacg cttgcggtaa cggtcgtcgc tgtaccgctg
     7981 cgtactgcgc tgttcggcgg tgtcaccggt agcggagccg atttgttcgt tgcctggatg
     8041 cactcgatgg ggcaaaacct ggtggagtcg gtggcgatta cagtgatagg cgcgaacctg
     8101 gtcgataaaa ttcttacagc ggtgattgtc tggcttttac tgcgccagct gccgattcgc
     8161 accacgcgcc attttccggc aatggctgcc gtgcgctaaa tgcatccatt tacctcatta
     8221 acactgtggg cattggcggc ctgcaccacg cttatcctgc ccgcgcagac cattttgccg
     8281 atctatagcg cggcgacctt tttttgtctt attgcgctaa aggccacgcg gcggcaggca
     8341 aaatatgtcg cctggctgat gttctcgctg ggagccgggc tgtggctggt acacggtggc
     8401 tggctaacag aatggctaag cggcacgccg cgttcgccgg agcgttggac gcatgctatt
     8461 acgctctggt tgcggattct ggcgattgtt tcaacctcac agttgtggat gcagtacgtc
     8521 cccgtgcaat gttttatccg cgctctgttt gcttctcgcc tgccgccagg cgtcgcttat
     8581 ctgtttgccg gaccgttatt ggtcgttgag cagttaaaac ggcaattagc gattattcat
     8641 gaagcgcagc gtgcccgcgg cgtgccgctg gatgagggct ggtatcagcg tttacgtgcg
     8701 atgcccgcgt taattattct gctaacacac aatgcgctta acgatctggc tgtccgtggt
     8761 gcggcgctgg atatgcgggc atttcggatc cacaaccgac gaacaacgtt gtgggcacca
     8821 gccgatagca ccctacaacg cgtagcgcgt tacaccatga tcttgctgat gctaacagaa
     8881 ttcggagcgt ggatatggtt acgttagaac agttccgtta ttgcccgaca cactcaacgc
     8941 atcctccgtt ctgttatgac tttcactatg tcaaaccggg gatggtcgcc atctttggtg
     9001 acaacggcag tggcaaaagt acgctggcac agctgatggc gggctggtat ccggactttc
     9061 tgccaggaga gataacaggc acggggacgc tcctcggtac gcccattggg catctcccgc
     9121 ttaatgaaca atccgccacc atccagctcg tccagcaatc gccctatttg cagctctccg
     9181 gctgtacctt tagcgtggaa gaagaagttg cctttgggcc agaaaatctg tgtcttgctg
     9241 aaacagagat tatggcgcgg attgatgccg ccctgactct gaccgagtgc cagccgttgc
     9301 gccatcgtca cccggcaaca ctctccggtg gagagacaca acgcgtggtc atcgcctgcg
     9361 ccatcgcgat gcagccaaag ctcttaattc ttgatgaagc ctttagtcgc ctgacgccgc
     9421 aggccagcga aatgctgttg cagcgcttac aacactgggc gcttgaacgt ggttcgctga
     9481 ttattctttt tgaacgtcat cgcacacctt tcctgaacta ctgccagcaa gcgtggcaat
     9541 tgcaaaacgg agcgctccag ccactatgtt aacgttaaat cagatatctt atcgctggcc
     9601 tagcgcagca acggattgtc tttgcgacat ttctctgcaa ctcaaacaag gtgaatggtt
     9661 ggcgctgacc ggcgacaatg gcgcgggaaa atcgacgctg ttacgagtaa tggcaggtct
     9721 tcttacgccc acagccggta cggttatgct gcaacaacag gcgatgaaaa acctgaaaaa
     9781 ccgccagcgc gcggcaaagg ttggcgtact gtttcaggaa gcggaaaacc aactttttca
     9841 cagcaccgtc gctgacgaga tcgcatttgg tttgaaactg caaaaatgcc cggcggatga
     9901 aatcactcaa cgtacccatg ctgccctgca atgttgtcag ttagcagaca ctgccaactc
     9961 gcacccgttg gatctgcata gtgcgcagcg acggatggtc gccgtcgcct gcctggaagc
    10021 actctctccg ccactattac tgctggacga gccgagtcgg gattttgacg aaaattggtt
    10081 gagcgtgttt gaaagctggc tggagaagtg ccgccagcga ggcacgagcg tggtagcaat
    10141 cagtcatgac gccgcattta cccggcgtca tttctctcgt gtggtgcgac tggaagatgg
    10201 tgtaatcagg aacgttaatc caccagacga tattcacccg tagcggtcat cattaaggtc
    10261 aaatttgcag gcagactctc ttccagcacc cggcgaacat tcggcccgtc ggtacggtca
    10321 taaaaggctt ctgcatctgc cagcgatacc cctcccgcca gcttgcgccc caccagacgc
    10381 tcccgcaacg cagtagctgg cgcgttgata aagatcgaga aatcacaaaa ttgcgcaagc
    10441 tggcaccact tctcgtcgtc cagcaacaac cagtttcctt cgacgatgac gagtggtgcg
    10501 gtaacgtgca acgcatcttc aacaggatca tgcttttgtc gatcgtactg cggccacgta
    10561 caatcccctt ccaccacctg gcgcagattt tccgccagtt tcgcaacgtc aaatgtctct
    10621 ggtgcgcctt tgaaggggcg caattgatgc gcgtccagcc agctattgta atggtgaaaa
    10681 ccatccatcg ggagcgtctg aatagcgggc agctccgggt cttgttgcac gagatattcc
    10741 cagaaggtgg ttagggtaga tttacccgtc cccggcggag cgcaaagaaa aaccaccgta
    10801 cgccgctgcg gatttaccgt ctgtaaggcc gccagcatac gcagcaacgg tttatgcacg
    10861 cgttctatct cttcatcatg gtactgcgct tgcacgttca gcccattcac cgttaattca
    10921 attttcacgg cgttaattcc tttaaggtgg tcgttactgc cagcgtcagc gccgttttga
    10981 ccattccgct aaaacgcgcc
//
LOCUS       DAZPAC010000133        10994 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZPAC010000133 DAZPAC010000000
VERSION     DAZPAC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10994
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.133"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..101
                     /locus_tag="ACHUPM_002913"
     ncRNA           <1..101
                     /ncRNA_class="other"
                     /locus_tag="ACHUPM_002913"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            221..562
                     /locus_tag="ACHUPM_002914"
     CDS             221..562
                     /locus_tag="ACHUPM_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR0870634.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(553..1479)
                     /locus_tag="ACHUPM_002915"
     CDS             complement(553..1479)
                     /locus_tag="ACHUPM_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0870635.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            1569..2567
                     /locus_tag="ACHUPM_002916"
     CDS             1569..2567
                     /locus_tag="ACHUPM_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR0870636.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2564..2782)
                     /locus_tag="ACHUPM_002917"
     CDS             complement(2564..2782)
                     /locus_tag="ACHUPM_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR0870637.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2784..4799)
                     /gene="ligA"
                     /locus_tag="ACHUPM_002918"
     CDS             complement(2784..4799)
                     /gene="ligA"
                     /locus_tag="ACHUPM_002918"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR0870638.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4870..5856)
                     /gene="zipA"
                     /locus_tag="ACHUPM_002919"
     CDS             complement(4870..5856)
                     /gene="zipA"
                     /locus_tag="ACHUPM_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR0870639.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6086..6847
                     /gene="cysZ"
                     /locus_tag="ACHUPM_002920"
     CDS             6086..6847
                     /gene="cysZ"
                     /locus_tag="ACHUPM_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR0870640.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7032..8003
                     /gene="cysK"
                     /locus_tag="ACHUPM_002921"
     CDS             7032..8003
                     /gene="cysK"
                     /locus_tag="ACHUPM_002921"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR0870641.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8387..8644
                     /gene="ptsH"
                     /locus_tag="ACHUPM_002922"
     CDS             8387..8644
                     /gene="ptsH"
                     /locus_tag="ACHUPM_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR0870642.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8689..10416
                     /gene="ptsI"
                     /locus_tag="ACHUPM_002923"
     CDS             8689..10416
                     /gene="ptsI"
                     /locus_tag="ACHUPM_002923"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR0870643.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10457..10966
                     /gene="crr"
                     /locus_tag="ACHUPM_002924"
     CDS             10457..10966
                     /gene="crr"
                     /locus_tag="ACHUPM_002924"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR0870644.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2676 a         2838 c         2735 g         2745 t
ORIGIN      
        1 gaacctgcag caggtttgag ttgagcgcag cgaaacaacg gagccgctcg cggcgacggc
       61 ccgaagggcg agcgaagcga gtcatcctgc acgacccacc aatgtaaaaa agcgccctaa
      121 aggcgctttt ttgctatctg cgatttgtaa gattgatttt tcttctcaag ttccttgttc
      181 ttctgccgat attacctcgt acagtgacga ggactgaaca atggccacta tctcttcaac
      241 ttcaattcct tccatacaaa cacagagtag taacagggca tcacaaggta gcgatgttgc
      301 ttcaaaaatc gcgcggataa gtcagcaaat catcaaactc actcaacaaa ttaaagagat
      361 tgtagacact agcggcagtg ctgaagacaa acaaaaacag gccgaattga ttcaacagca
      421 aatcacccta ctggaaacac aactggcgca gttgcagaga cagcaggcag aaaaggcgca
      481 ggagaaagag cagcgtttgt ctctgaatgt atcccttcta aatcctgttg aaaatacgac
      541 tcatatcgat atctatatct gatacaacgg agccccttcc cgaccagcca ttagcgcatt
      601 gccctggtct cgtaccacgt cccataacgc ttcagcggcg gtggaaagtg aacggttctt
      661 cctgcgcacc agcatcaact ggcgttcaac aaccggcgta atgcgcttta ccaccagtgg
      721 gctaccttca ggcaatggta gcgccagtgc gggaagaata ctgatgccga tgcctgcggc
      781 taccatcggg aacagcgtcg ccggatgtcc aatctcctgc acaatattcg cctgaatacc
      841 attgcgcgcc agcgctgcgt caatcagcgg tcggctgcct gacgcgtaat cctgcaacac
      901 caatttcgct ccctgtaatg cctgccaggg tacataatct tccacggcca aagcgctatc
      961 gcgatggcaa agcagaaaga aaggctcgga aagaatcgct tcacattgca gatcgcccac
     1021 tgggcctgga tcgatgacga tgcaaaaatc gacatctccc tggcgaatac tttccatcac
     1081 ccactgctgc ggacgatcgt gcaggacaaa ctgaatatct ggatagcggc gatggctttc
     1141 agcaatgcat tgcggaataa gatgcgcgga aatggtctgg ctggcagcga cccgaacttt
     1201 tccgctcagt tgttgcccca tacgcccggt atcgcgcaac gtgctgttaa gttcatccag
     1261 cagtcgctca agacgcaaag ccaactgctg ccctgcatct gtaagcacca cttcacgcgt
     1321 ggttctgtcc agcaggcgaa caccagtatg attttccagc tccttcacac tgtgacttac
     1381 tgccgactgg ctcaagccga tacgctctcc tgcacgacta aaacttttct cctgcgctac
     1441 tgtgacgaaa acctttaatt gttttaaaga ataattcatc tgttttcttc atgaatggat
     1501 gcaataaatc aattttattt ctcaaactgg aagaagcaca atagaaccat cgatcatctg
     1561 gagtctttat gaaacttttt cgtatcctcg atcctttcac cttaaccctg atcacggtgg
     1621 tgttgctggc ctctttcttt ccggccagag gcgatttcgt ccccttcttt gaaaatctga
     1681 ccaccgcagc tattgccctg ctgttcttta tgcacggcgc gaagttgtcg cgtgaggcga
     1741 ttattgctgg cggtggtcac tggcgactgc atttgtgggt aatgtgcagc accttcgtgc
     1801 tgtttccgat tctgggtgta ctgtttgcct ggtggaaacc ggtaaatgtc gacccgatgc
     1861 tctactccgg ttttctctac ttgtgcattc tcccggctac cgtgcagtct gcaatcgcct
     1921 tcacgtcaat ggcgggcggt aacgtcgcgg cggcggtttg ttctgcgtca gcatccagcc
     1981 tgctggggat tttcctttca ccattgctag ttggtctggt gatgaatgtt cacggtgcag
     2041 ggggcagcct tgagcaggtc ggtaaaatta tgctgcaact gctgctgccg tttgtgttgg
     2101 ggcatctttc ccggccgtgg attggtgact gggtgtcgcg caataaaaaa tggattgcga
     2161 aaactgacca gacgtccatt ctgttggtgg tttatacagc gttcagcgaa gccgtcgtta
     2221 atggtatctg gcataaagtt ggctggggat cattgctgtt tatcgtggtg gtcagctgcg
     2281 ttcttctggc tatcgtgatt gtagttaacg tctttatggc acgccgactg ggcttcaata
     2341 aggcagatga aattactatc gtcttttgtg gttcgaaaaa gagtctggca aatggcatcc
     2401 cgatggcaaa cattctgttc cccacatcgg tgatcggtat gatggtgctg cccctgatga
     2461 ttttccatca gatccaattg atggtctgtg cggtgctggc gcgtcgatac aaacgccaga
     2521 ccgaacagtt acaggcgcag caggaaagca gcgccgataa agcttaaagc ggacgcttca
     2581 ggggctggac cagttgcgtc agcccctcgg ttttaatcag cagcgtgatt tgcattagct
     2641 caccgagctt ccctgccggg aactcatctt tgcgggcaaa ccacagcaga tactcctccg
     2701 gcaggtcgat taagcgacgc cctttgtatt tgccaaacgg cattatcgta ttggctattt
     2761 caatcagctg ctctttttcc atctcagcta cccagcaaac gcagcatttc cgcttcgtcg
     2821 atgacttcaa tgcccagttc ctgcgccttc gccagtttag atcctgcagc ttcacccgct
     2881 atcaccagat cggttttctt cgacacgctg cccgcgactt tcgcgcccag ttcgaccagt
     2941 cgagctttag cgtcatcacg cgacatctgg cttaagctgc ccgtaagcac cacggtttta
     3001 ccagcaaacg ggctgtcaat ctcttccgcg ttgataacga tcggcgcagg ccagtgaaca
     3061 ccttccgcca acagctcgct gatgacattg cggttgcttt cttcggcaaa gaagttgtga
     3121 acgtgggatg caacgacaat gccaacatca ggcacctttt gcagctcttc aatcgaagcg
     3181 gcttccagca cttccagcgt gccgaaatat gccgccagac ctgctgcggt ggcctcgccg
     3241 acttcacgga tgccaagtgc atagaggaag cgagcaaagg tggtttcttt cgctttttcc
     3301 agcgcgttaa ccacgttttg tgccgatttt ggccccatac gctccagtcc ggtcagtttg
     3361 cctgcggtga gtttgaacag atctgccgga gtgtggacat attctttttc aaccagctga
     3421 tcgatgattt tgtcgcccat tccgtcaaca tccatcgcac ggcgggaaac aaagtgtttc
     3481 agcgactctt tacgctgcgc accgcaaatc aggccaccgg tacagcgggc aaccgcttca
     3541 ccttccacac gctcaacgtc agaaccacat accggacaat gcgtcgggaa tacaacctca
     3601 cgggtatctt ccgggcgttc agaaagcacg acgttaacca cctgcgggat cacgtcgcca
     3661 gcgcggcgaa tcaccacttt atcgccaatg cgtaaaccaa gacgttcgat ttcatccgca
     3721 ttgtgtaagg ttgcgttact caccagcacg ccagcaacat ggacaggttc cagacgcgca
     3781 acaggcgtaa tcgcgccagt acgcccaacc tgaaactcga cgtcacgcac aaaggtcatc
     3841 tgctcctgcg ccgggaattt aaacgctacc gcccagcgcg gggcacgcgc gacaaagcca
     3901 agctgctcct gctgttccag tgagttgacc ttaatcacca cgccgtcgat atcaaagccc
     3961 agcgtcgggc ggtcttcttc cactttgtga tagaacgcca gcacttcttc cgccgattca
     4021 caaagcgtta cccgatcgct gaccggcaac ccccactttt taaattgcag taaacggcca
     4081 agatgagtat ccggcagctc gccaccttcc agaacaccaa cgccatagca gaaaaaagtg
     4141 agcggtcgct tcgctgtaat acgcggatca agctgacgca gtgaaccagc tgccgcatta
     4201 cgtgggttag caaaaacttt cccgcccgtg cgtcgcgcat cttcgttaat cttttcgaac
     4261 cccgcctgcg gcaggaacac ttcaccacgc acttccagac gcgccgggat attctctccg
     4321 tgcagcttca gcggaatggc gcgaatagta cgcacattag acgtgatatc ttccccggtg
     4381 gtgccatcgc cacgggtcgc ggcactgact aaaacgccat tttcatacag aatactgacg
     4441 gcaaggccat ccagcttcag ctcacagcac caggtgactt tctcgttgtt tttcagacgg
     4501 tcctgcacac gtttgttgaa agcaagaaag ctttcttcat caaaaacgtt atccagtgac
     4561 agcattggta cttcatggcg tatctggctg aaagccgcca gcggcgcagc acctacacgt
     4621 tgggtaggcg aatcaggcgt aatcagttct ggatgtttgg tttccagctc gcgcagttcg
     4681 cgcatcagcc tgtcgtattc agcgtcggga atttccggcg catccatcac atgataaaga
     4741 tattcatgat ggcgaagcgt cgttcgcagt tctgtcagtt gttgttcgat tgattccata
     4801 tcgcaccatc aatgctaaaa acccccgaca agcgggggtt cgaagaggag ttaatttgcc
     4861 ttaagtgtat caggcgttgg cgtctttgac ttcgcggatg atgtcctggt actcgcgcaa
     4921 tttctgcgga gtcatcatac ggcgctgatc gtcaagcacg acaccgccca cttcatcggc
     4981 aatatgctgc gcagattgca gcatcagctt gaagttctgc agctcgtcac cgtaagacgg
     5041 tacctgcata aagatagtga cacccggagt agtgaaatcc ttcatttcag gatcaaaggt
     5101 tcccggtttc accatattcg ccaggctgaa taacgccggg ccgctgccat ccgggctaag
     5161 atgacgatgg taaatattca tatcgccaaa aatgaagccc gcttgttgaa tgctgttaag
     5221 aagcagttca ccgtttagct cgctaccgtg atgcgccgcg acgttcatga taatcaccgc
     5281 ttctttgcgc ttcggtttat ccataactgg agccggttcc gctacaggct caggctgtgg
     5341 tgccgctacg ggttctgcag gctggaaagc ctgttgtgcc ggttgcggtg ctgaatgcac
     5401 aggctgcggc gctggcgcga cctgtggcga aactggctgc tgcaacggct gttcaggctg
     5461 cggctgatag gcaggctgct gcatcggctg cgctggacgc ggagcatgtt gcggcggtac
     5521 ctgcgcttca ggcggctgct ggatcggttg acgcggctgc gcagacgcat aaggcggttg
     5581 gtactggtgt tgcggcgacg gacgagcagc ctcatgctcc tgagcgttag ccggggcatg
     5641 attcacgcgg tgaacacgaa cctcaccaac gccctcatca tcttcgacat cctcgtcata
     5701 agaatcgtcg tcacgttttg acttcattcg ttttaatggc cgatcgcgga acatagaaga
     5761 tcgttcttta cggctggtcc agaaaccatg taccagtaaa gcgattatgg cgatcgcgcc
     5821 aacaatgatt aatatcagac gcaaatcctg catcattata ttctctgttg ttctaacacc
     5881 ttgccaccac ggcaaacatt tactcactaa gagtatttgc cgattacctc aagtgcaagt
     5941 gcactattaa ctttcacagc acaaagatag atgaaatcgt gctttttgct gttttttcga
     6001 acatatccta actgtccatt gcgcaattac ccggtaaaat acgcagaatt ttcctgggat
     6061 tggtcaaaag gagctcatcc tgattatggt ttcatcattc acatctgccc cacgcagcgg
     6121 tttttactat tttgcgcaag gctggaagct cgtctcgcaa cctgggattc ggcgtttcgt
     6181 tattttaccg ctgctggtca atattttgtt gatggggggc gcattctggt ggctctttac
     6241 acagctcgat gtctggatcc cgactctcat gagttacgtt ccggactggc tacaatggct
     6301 gagttatctg ttgtggcctc tggctgtcat ctctgtgctg ttagtgtttg gctatttctt
     6361 ctccacgatt gctaactgga ttgccgctcc gtttaacggt ttattggctg aacaactgga
     6421 agcacgattg actggcgcta caccgccaga taccgggatt ttcggtatca tgaaagatgt
     6481 gccgcgaatc atgaaacgcg aatggcaaaa atttgcctgg tatctgccgc gcgcaattgt
     6541 attactaatt ctttacttca tccctggtat tgggcaaacc gtcgcgccgg tactgtggtt
     6601 cctgtttagc gcctggatgt tagccatcca gtattgcgat taccccttcg ataaccacaa
     6661 agtgccgttt aaagagatgc gcaccgccct gcgcacacgc aaaatcacca atatgcagtt
     6721 tggtgcttta accagcctgt ttacgatgat cccgctgctt aatctgttca tcatgcccgt
     6781 tgccgtttgt ggcgcgacgg cgatgtgggt cgattgctat cgcgataaac acgcgatgtg
     6841 gcggtaacaa tctaccggtt attttgtaaa ccgtttgtgt gaaacagggg tggcttatgc
     6901 cgccccttat tccatcttgc atgtcattat ttcccttctg tatatagata tgctaaatcc
     6961 ttacttccgc atattctctg agcgggtatg ctacctgttg tatcccaatt tcatacagtt
     7021 aaggacaggc catgagtaag atttttgaag ataactcgct gactatcggt cacacgccgc
     7081 tggttcgcct gaatcgcatc ggtaacggac gcattctggc gaaggtggaa tctcgtaacc
     7141 ccagcttcag cgttaagtgc cgtatcggtg ccaacatgat ttgggatgcc gaaaagcgcg
     7201 gcgtgctgaa accaggcgtt gaactggttg aaccgaccag cggtaatacc gggattgcac
     7261 tggcctatgt agctgccgct cgcggttaca aactcaccct gaccatgcca gaaaccatga
     7321 gtattgaacg ccgcaagctg ctgaaagcgt taggtgcaaa cctggtgctg acggaaggtg
     7381 ctaaaggcat gaaaggcgca atccaaaaag cagaagaaat tgtcgccagc aatccagaga
     7441 aatacctgct gctgcaacaa ttcagcaatc cggcaaaccc tgaaattcac gaaaaaacca
     7501 ccggtccgga gatctgggaa gataccgacg gtcaggttga tgtatttatt gctggcgttg
     7561 ggactggcgg tacgctgact ggcgtcagcc gctacattaa aggcaccaaa ggcaagaccg
     7621 atcttatctc tgtcgccgtt gagccaaccg attctccagt tatcgcccag gcgctggcag
     7681 gtgaagagat taaacctggc ccgcataaaa ttcagggtat tggcgctggt tttatcccgg
     7741 ctaacctcga tctcaagctg gtcgataaag tcattggcat caccaatgaa gaagccattt
     7801 ctaccgcccg tcgtctaatg gaagaagaag gtattcttgc aggtatctct tctggagcag
     7861 ctgttgccgc ggcgttgaaa ctacaagaag atgaaagctt taccaataag aatattgtgg
     7921 ttattctacc atcatcgggt gagcgttatt taagcaccgc attgtttgcc gatctcttca
     7981 ctgagaaaga attgcaacag taatgccagc ttgttaaaaa tgcgtaaaaa agcacctttt
     8041 taggtgcttt tttgtggcct gcttcaaact ttcgcccctc ctggcattga ttcagcctgt
     8101 cggaactggt atttaaccag actaattatt ttgatgcgcg aaattaatcg ttacaggaaa
     8161 agccaaagct gaatcgattt tatgatttgg ttcaattctt cctttaacgg cataatgttt
     8221 aatgacgtac gaaacgtcag cggtcaacac ccgccagcaa tggactgtat tgcgctcttc
     8281 gtgcgtcgcg tctgttaaaa actggcgcta acaatacagg ctaaagtcga accgccaggc
     8341 tagactttag ttccacaaca ctaaacctat aagttgggga aatacaatgt tccagcaaga
     8401 agttaccatt accgctccga acggtctgca cacccgccct gctgcccagt ttgtaaaaga
     8461 agctaagggc ttcacttctg aaattactgt gacttccaac ggcaaaagcg ccagcgcgaa
     8521 aagcctgttt aaactgcaga ctctgggcct gactcaaggt accgttgtga ctatctccgc
     8581 agaaggcgaa gacgagcaga aagcggttga acatctggtt aaactgatgg cggaactcga
     8641 gtaatttccc gggttctttt aaaaatcagt cacaagtaag gtagggttat gatttcaggc
     8701 attttagcat ccccgggtat cgctttcggt aaagctctgc ttctgaaaga agacgaaatt
     8761 gtcattgacc ggaaaaaaat ttctgccgac caggttgatc aggaagttga acgttttctg
     8821 agcggtcgtg ccaaggcatc agcccagctg gaaacgatca aaacgaaagc tggtgaaacg
     8881 ttcggtgaag aaaaagaagc catctttgaa gggcatatta tgctgctcga agatgaggag
     8941 ctggagcagg aaatcatagc cctgattaaa gataaacaca tgacagctga cgcagctgct
     9001 catgaagtta tcgaaggtca ggcttctgcc ctggaagagc tggatgatga atacctgaaa
     9061 gaacgtgcgg ctgacgtacg tgatatcggt aagcgcctgc tgcgcaacat cctgggcctg
     9121 aagattatcg acctgagcgc cattcaggat gaagtcattc tggttgccgc tgacctgacg
     9181 ccgtccgaaa ccgcacagct gaacctgaag aaggtgctgg gtttcatcac cgacgcgggt
     9241 ggccgtactt cccacacctc tatcatggcg cgttctctgg aactgcctgc tatcgtgggt
     9301 accggtagcg tcacctctca ggtgaaaaat gacgactatc tgattctgga tgccgtaaat
     9361 aatcaggttt acgtcaatcc aaccaacgaa gttattgata aaatgcgcgc tgttcaggag
     9421 caagtggctt ctgaaaaagc agagcttgct aaactgaaag atctgccagc tattacgctg
     9481 gacggtcacc aggtagaagt atgcgctaac attggtacgg ttcgtgacgt tgaaggtgca
     9541 gagcgtaacg gcgctgaagg cgttggtctg tatcgtactg agttcctgtt catggaccgc
     9601 gacgcgctgc ccactgaaga agaacagttt gctgcttaca aagcagtggc tgaagcgtgt
     9661 ggctcgcagg cggttatcgt tcgtaccatg gacatcggcg gcgacaaaga gctgccatac
     9721 atgaacttcc cgaaagaaga gaacccgttc ctcggctggc gcgctatccg tatcgcgatg
     9781 gatcgtaaag agatcctgcg cgatcagctc cgcgctatcc tgcgtgcctc ggctttcggt
     9841 aaattgcgca ttatgttccc gatgatcatc tctgttgaag aagtgcgtgc actgcgcaaa
     9901 gagatcgaaa tctacaaaca ggaactgcgc gacgaaggta aagcgtttga cgagtcaatt
     9961 gaaatcggcg taatggtgga aacaccggct gccgcaacaa ttgcacgtca tttagccaaa
    10021 gaagttgatt tctttagtat cggcaccaat gatttaacgc agtacactct ggcagttgac
    10081 cgtggtaatg atatgatttc acacctttac cagccaatgt caccgtccgt gctgaacttg
    10141 atcaagcaag ttattgatgc ttctcatgct gaaggcaaat ggactggcat gtgtggtgag
    10201 cttgctggcg atgaacgtgc tacacttctg ttgctgggga tgggtctgga cgaattctct
    10261 atgagcgcca tttctatccc gcgcattaag aagattatcc gtaacacgaa cttcgaagat
    10321 gcgaaggtgt tagcagagca ggctcttgct caaccgacaa cggacgagtt aatgacgctg
    10381 gttaacaagt tcattgaaga aaaaacaatc tgctaatcca cgagatgcgg cccaatttac
    10441 tgcttaggag aagatcatgg gtttgttcga taaactgaaa tctctggttt ccgacgacaa
    10501 gaaggatacc ggaactattg agatcattgc tccgctctct ggcgagatcg tcaatatcga
    10561 agacgtgccg gatgtcgttt ttgcggaaaa aatcgttggt gatggtattg ctatcaaacc
    10621 aacgggtaac aaaatggtcg cgcctgtaga cggtaccatt ggtaaaatct ttgaaaccaa
    10681 tcacgcgttc tctatcgaat ctgatagcgg cgttgaactg ttcgtccact tcggtatcga
    10741 caccgttgaa ctgaaaggcg aaggcttcaa gcgtattgct gaagaaggtc agcgcgtgaa
    10801 agttggcgat actgtcattg aatttgatct gccgctgctg gaagagaaag ccaagtctac
    10861 cctgactccg gttgttatct ccaacatgga cgaaatcaaa gaactgatca aactgtccgg
    10921 tagcgtaacc gtgggtgaaa ccccggttat ccgcatcaag aagtaattct tgccgcagtg
    10981 aaaaatggcg ccca
//
LOCUS       DAZPAC010000134        10986 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZPAC010000134 DAZPAC010000000
VERSION     DAZPAC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10986
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.134"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            51..812
                     /gene="adiY"
                     /locus_tag="ACHUPM_002925"
     CDS             51..812
                     /gene="adiY"
                     /locus_tag="ACHUPM_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR0870645.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            949..2286
                     /gene="adiC"
                     /locus_tag="ACHUPM_002926"
     CDS             949..2286
                     /gene="adiC"
                     /locus_tag="ACHUPM_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR0870646.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2390..4033
                     /gene="eptA"
                     /locus_tag="ACHUPM_002927"
     CDS             2390..4033
                     /gene="eptA"
                     /locus_tag="ACHUPM_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR0870647.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            4030..4698
                     /gene="basR"
                     /locus_tag="ACHUPM_002928"
     CDS             4030..4698
                     /gene="basR"
                     /locus_tag="ACHUPM_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR0870648.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4699..5799
                     /gene="pmrB"
                     /locus_tag="ACHUPM_002929"
     CDS             4699..5799
                     /gene="pmrB"
                     /locus_tag="ACHUPM_002929"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR0870649.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(5775..5864)
                     /gene="pmrR"
                     /locus_tag="ACHUPM_002930"
     CDS             complement(5775..5864)
                     /gene="pmrR"
                     /locus_tag="ACHUPM_002930"
                     /inference="COORDINATES: protein motif:HMM:NF033224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR0870650.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5976..7478)
                     /gene="proP"
                     /locus_tag="ACHUPM_002931"
     CDS             complement(5976..7478)
                     /gene="proP"
                     /locus_tag="ACHUPM_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR0870651.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7742..8608)
                     /locus_tag="ACHUPM_002932"
     CDS             complement(7742..8608)
                     /locus_tag="ACHUPM_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR0870652.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(8605..10833)
                     /gene="crfC"
                     /locus_tag="ACHUPM_002933"
     CDS             complement(8605..10833)
                     /gene="crfC"
                     /locus_tag="ACHUPM_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR0870653.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2746 a         2867 c         2692 g         2681 t
ORIGIN      
        1 gctttgcttt agcgagaact ggtcttttat tcgcactcag gagtacatgt atgaggattt
       61 gcagcgacca accttgtatt gttttattga ctgaaaaaga tgtctggata agggtgaatg
      121 ggaaagaacc tattagcctt aaagctaacc atatggcgtt attaaattgt gaaaataata
      181 ttatcgacgt ctcctctctt aacaacactt tggttgctca tattagtcac gacatcatca
      241 aagattacct ccggtttctg aataaagatc tctcgcaaat accagtatgg caacgtagcg
      301 ctacgcccat actcaccctg ccatgcctga cgccagacgt ctttcgcgtt gccgcgcaac
      361 acagcatgat gcccgcagaa actgagtcag aaaaggaacg aacacgcgca ttattattca
      421 cggtgctatc ccgttttctc gacagtaaaa aattcctttc attaatgatg tatatgttac
      481 gtaattgtgt aagtgacagc gtttatcaaa ttattgaaag cgatattcac aaagactgga
      541 atcttagtat ggtagccagt tgtttatgtc ttagcccaag tctgttaaag aaaaagctga
      601 aaagcgaaaa caccagttat agccaaataa tcaccacctg ccgcatgcgt tatgccgtaa
      661 atgaattaat gatggacggt aaaaatatct ctcaggtatc acagtcctgc ggctacaaca
      721 gtacgtcgta ctttatttct gtctttaaag acttctacgg tatgacgccg ctgcattatg
      781 ttagtcagca cagagaacga actgtcgcct gatttttaac cttaacgaag agctatatta
      841 ataacggcat cagcgataac ccggtcgata ataattcaac tatcgaatgc aggcgtatga
      901 tatgacgtaa tttattgtca cgaagctcgc cttcgcagga gtttaattat gtcttcggat
      961 gctgatgctc acaaagtggg cttaatcccc gtcaccctga tggtgtcggg gaatattatg
     1021 gggtcaggtg tttttctgtt acctgcaaac ctggcctcca ctggcgggat tgccatttat
     1081 ggatggttgg tgacgattat cggtgcgctg gggctctcga tggtatacgc caaaatgtcg
     1141 ttcctcgatc caagtcctgg tggttcttac gcttacgccc gccgctgctt tggcccgttt
     1201 ctcggttatc aaaccaacgt cctctactgg ctggcctgct ggatcggcaa tatcgctatg
     1261 gtggtcattg gcgtaggata tttaagttac ttcttcccga ttctgaaaga tccattggta
     1321 ttaaccatca cctgcgtcgt ggtgctgtgg atcttcgtcc tgctgaacat tgtcggtccg
     1381 aaaatgatca cccgtgtgca ggccgttgcc accgtgctgg cgctgatccc catcgtcggg
     1441 attgccgtat ttggctggtt ctggttccgt ggtgaaacct atatggcggc atggaacgtc
     1501 agcggcctgg gcaccttcgg tgcgattcaa agtaccctta acgttacgct gtggtcgttc
     1561 atcggtgtgg aaagtgcctc cgttgccgca ggtgtggtga aaaacccgaa acgcaatgtc
     1621 cctatcgcca ccattggtgg ggtattgatt gccgccgttt gctatgtgct ctccaccacc
     1681 gcgattatgg ggatgatccc taatgccgca ctgcgcgttt ctgcctcgcc attcggtgat
     1741 gccgcacgga tggcgttggg tgacaccgct ggggccattg tttccttctg cgcagctgcg
     1801 ggttgcttag gttcactggg cggctggacg ttgctggcgg gtcaaacggc gaaagccgct
     1861 gccgatgacg gactgttccc accgattttt gcccgtgtaa ataaagcggg tacgccagtg
     1921 gcggggctga ttatcgtcgg tattttgatg accatcttcc agctcagtag catttcgcca
     1981 aacgcaacca aagagttcgg tctggtatct tccgtgtcgg tcatctttac actggtgcca
     2041 tatctttaca cctgtgcggc gttactgctg ctcggccacg gtcactttgg taaagcacgc
     2101 ccggcatatc tggctgttac taccattgcc ttcctctact gcatctgggc cgtggtgggg
     2161 tccggggcga aagaggttat gtggtcattt gtcaccctga tggtcatcac cgccatgtat
     2221 gcgctgaatt acaaccggct acataaaaac ccgtatccct tagatgcacc aataagcaaa
     2281 gattaattcc ccttaatcca gcaaacataa aagccaacct taagaactta aggttggctt
     2341 aattttgctt tgcgagcata tgcgcacttt gttcgatgga aacaccgtga tgttgaagcg
     2401 cctactaaaa agcccctctt tgaatttact cgcctggcta ttgttggccg ctttttatat
     2461 ctctatctgc ctgaatattg ccttttttaa acaggtgttg caggcgctgc cgctggattc
     2521 gctgcataac gtactggttt tcttgtcgat gccggtcgtc gctttcagcg tgattaatat
     2581 tgtcctgaca ctaagctctt tcttatggct taatcgacca ctggcctgcc tgtttattct
     2641 ggttggcgcg gctgcacaat atttcataat gacttacggc atcgtcatcg accgctcgat
     2701 gattgccaat attattgata ccactccagc agaaagttat gcgctgatga caccgcaaat
     2761 gttattaacg ctgggattca gcggcgtgct tgctgcgctg attgcctgct ggctaaaaat
     2821 caaacctgcc acctcgcgtc tgcgcagtgt tcttttccgt ggagccaata ttctggtttc
     2881 tgtactgctg attttgctgg tcgccgcact gttttataaa gactacgcct cgttgttccg
     2941 caataacaaa gagctggtga aatccttaag cccctctaac agcattgttg ccagctggtc
     3001 atggtactcc catcagcgac tggcaaatct accgctggtg cgaattggtg aagacgccca
     3061 ccgcaacccg ttaatgcaga acgaaaaacg taaaaatttg accatcctga ttgtcggcga
     3121 aacctcgcgg gcggagaact tctccctcaa cggctacccg cgtgaaacta acccgcggct
     3181 ggcgaaagat aacgtggtct atttccctaa taccgcatct tgcggcacgg caacggcagt
     3241 ttcagtaccg tgcatgttct cggatatgcc gcgtgagcat tacaaagaag agctggcaca
     3301 gcaccaggaa ggcgtgctgg atatcattca gcaagcgggc atcaacgtgc tgtggaatga
     3361 caacgatggc ggctgtaaag gtgcctgcga ccgcgtgcct caccagaacg tcaccgcgct
     3421 gaatctgcct ggtcagtgca tcaacggcga atgctatgac gaagtgctgt tccacgggct
     3481 ggaagagtac atcaataacc tgcagggtga tggcgtgatt gtcttacaca ccatcggcag
     3541 ccacggtccg acctattaca accgctatcc gccgcagttc aggaaattta ccccaacctg
     3601 cgacaccaat gagatccaga cctgtaccaa agagcaactg gtgaacactt acgacaacac
     3661 gctggtttac gtcgactata ttgttgataa agcgattaat ctgctgaaag aacatcagga
     3721 taaatttacc accagcctgg tttatctttc tgaccacggt gaatcgttag gtgaaaatgg
     3781 catctatctg cacggtctgc cttatgccat cgccccggat agccaaaaac aggtgccgat
     3841 gctgctgtgg ctgtcggagg attatcaaaa acggtatcag gttgaccaga actgcctgca
     3901 aaaacaggcg caaacgcaac actattcaca agacaattta ttctccacgc tattgggatt
     3961 aactggcgtt gagacgaagt attaccaggc tgcggatgat gttctgcaaa cttgcaggag
     4021 agtgagtgaa tgaaaattct gattgttgaa gacgatacgc tgttattgca gggactgatt
     4081 ctggcggcgc aaaccgaagg ctacacgtgc gatggcgtaa caaccgcgcg gatggcggaa
     4141 caaagccttg aggccggtca ttacagcctg gtggtactgg atttagggtt acccgacgaa
     4201 gatggactgc attttctcgc ccgtatccgg cagaaaaaat acaccctgcc ggtactgatc
     4261 ctcaccgctc gcgatacgct gaccgacaaa atcgccgggc tggatgtcgg tgccgatgac
     4321 tatctggtga agccttttgc gctggaagag ttacacgccc gtatccgcgc cctgctacga
     4381 cgccataata atcagggcga aagtgagctg attgttggca atctgacgct gaatatgggt
     4441 cgccgtcagg tatggatggg cggtgaagag ttgattctga cgcccaaaga atatgctctg
     4501 ctgtcacggt taatgctcaa agcaggcagt ccggtacatc gggaaattct ctacaacgac
     4561 atctataact gggacaatga accctcgacc aacaccctgg aagtgcatat ccacaatctg
     4621 cgcgacaaag tgggcaaagc ccgtatccgc accgtgcgcg gctttggcta tatgctggtc
     4681 gcgaatgagg aaaactaatt gaatctgatg cgttttctgc gccgaccaat atcgctgcgc
     4741 caacggctga tattgaccat cggggccatt ttgttggtgt ttgagctgat cagcatcttc
     4801 tggctatggc atgaaagtac cgagcagatt cagctgtttg agcaggcgct acgcgacaat
     4861 cgcaacaacg atcgccacat catgcgtgag atccgcgagg cggtcgccag cctgattgtc
     4921 cccggcgtct ttatggtcag cctgacgcta tttatctgct atcaggcggt acgccgcatc
     4981 acccgcccgc tggcggagct gcaaaaagag ctggaagcgc gcaccgccga caacttaacg
     5041 cccattgcca ttcacagcgc caccctcgaa atcgaagcgg tggtttcggc gttgaacgat
     5101 ctggtcagtc gcctgacgag cacgctggat aacgaaaggt tatttaccgc tgacgtcgcg
     5161 cacgaactgc gaacgccact ggcgggggtg cgtttgcatc tggaactgct ggcgaaaacg
     5221 catcacattg atgtagcacc gttagtggca cggcttgatc agatgatgga gagcgtctcc
     5281 cagctgctgc aactggcgcg tgccggacag tcattttctt ccggtaatta tcaacatgta
     5341 aaactgctgg aagatgtcat tctcccctcg tatgacgaac tcagtaccat gctcgaccag
     5401 cgacagcaaa ccctgctatt gccagagagc gccgccgaca tcaccgttca gggtgatgca
     5461 accctgctgc ggatgttatt gcgaaacctg gtagaaaacg cccatcgtta cagcccacaa
     5521 ggcagcaaca ttatgattaa gctgcaagaa gatggcggag cggtcatggc cgttgaagat
     5581 gaaggaccgg gtattgatga gagtaaatgc ggggagttga gtaaagcgtt tgtacgtatg
     5641 gacagccgtt atggcgggat tggtctgggg ttaagtattg tcagccgcat tacacagttg
     5701 catcacgggc agtttttcct gcaaaaccgg caagagactt ccggcacgcg ggcctgggta
     5761 cggctgaaga aagatcagta cgtagcaaac cagatataaa gaaagctgct gaccaccagc
     5821 acactgaaca cggtagttaa actttcataa acacggtttt tcattgcact ctcctccgtt
     5881 aacctggagg agagtatgcg cgtcagagat taagtgaacc ttaagagttc aattggcctg
     5941 ataagacagc gtcacatcag gccatccgtt tcagcttatt catcaattcg cggatgttgc
     6001 tgcaccaggc gggtacgttt cgcctgcaaa tcggcaatct cgtggtcaat atcatcgatt
     6061 ttctgctcga tattatcgta atgctcgacg agaatttcct tcgcttcctg tatatctgac
     6121 gccgccggtg tcgcaccttt caacggacga tttgccgtct ctttcatggt tacgccggtg
     6181 attaaaccaa tcaccgccac taccatcagg taataggcag gcatcatcag attctgcgag
     6241 ctttcgacca gccaggccgc cagtgttggc gtcagaccgg caaccagcac cgaaatatta
     6301 aatgccgccg ccagcgcgct gtaacggata tgcgtcggga acatcgctgg caaggtagaa
     6361 gccataacgc ccgtaaagca gttaaggatc accgccagca tcagtaaccc ggcaaaaatc
     6421 aggccgatga cgttactgtt aatcagaata aacgccggga tcgccaacac aaacagggca
     6481 acactaccaa gtagcacaaa cggacgacgg ccaaaacggt cactcagcaa gcccatcacc
     6541 ggctggacaa acagcatacc gatcataatg gcgataataa tcagcacccc gtggtcttcg
     6601 gagtaatgca ggttatgcga caaataactc ggcatatagg tcagcaacat gtagtaagtc
     6661 acgttggtgg caattaccag accaatacat gtcaacaggc tgcgccagta tttagtggca
     6721 atctctttaa acgagacttt cgggccatcc tgcaaacctt cgcggtcgcc ctgttccagt
     6781 ttatcaacat gctgctggaa cgccggagtt tcttccagcg catggcgcag gtaaagcccg
     6841 ataatcccta acggcagagc aataaagaac ggaatacgcc agccccagtc gaggaagttc
     6901 gcttcgccga caatggtcga aattaacacc accacgcccg cacccagcac aaacccggca
     6961 atagaaccga agtccagcca gctgcccata aagccacgtt tacggtcagg ggagtattcc
     7021 gcaacaaata tcgacgcccc ggtatattca ccgccgaccg agaaaccttg tgccatctta
     7081 cagatcaaca gcagaatcgg tgcccaaata ccaatcgtgt cgtaggacgg tattaagcca
     7141 atacagaacg tactgatcga cataatcaca atagtgatag cgaggatctt ctggcgacca
     7201 tatttatcgc ccaacatacc aaagaagagc ccgccaagcg gtcgaatcag aaagggaacg
     7261 gagaaagtgg caagtgcagc aaccatctgc acgctggggt cagcccccgg gaaaaaaact
     7321 ttacctaatg cgtaagcaac aaaaccataa acaccaaaat cgaaccattc cattgcatta
     7381 cccagtgatg ctgcggtaat ggctttacgc agtttaccgt catcaataat ggtgacatca
     7441 cgaagggtaa tcggttttac ttttttcctt ttcagcatag ctttcctcgc agagttggcc
     7501 catttccgca aacgccagaa aaatcagcgc gacggccgca aaaggggagc gggaattgca
     7561 ggatgcaatc tctgtaacaa gcgtagcggg tttgcttaca ccctccggtt aaggaatgta
     7621 caatcaaacc agttgacgcc tgaattgggc agttaatgac ctttttaccc taccagtgtt
     7681 taaatttgtg atcaacttca cacatggatt tcgtttagga ctcattgatg taactgatta
     7741 tttacgccaa atttacagag aagatttcct cgaccattgc ccccaccaaa cgctgcgctg
     7801 aacagagtcg tggcatcccc agcgcgactg tttgccagcg ttgggtcact gaccagcaaa
     7861 cgggctgttt atctgccgtg gaccactttc ccagccaact taaaaaatca gcatgctccc
     7921 ctcccgaaga ggcaaaccac tgatggtaat agtgacgcgt ggtcggtgcc gcattgatac
     7981 tctctgccag atgttgtgtc gctttctcgc gcaaatcacc cagtaacaac accaccgctt
     8041 gcgggcttcc cagacggcag gcatcgccaa atgcacccca gcgcaactcc tccagaacga
     8101 taaaacagcg cccaggcaac gaccaggcgt tatacgcccc agcacgccag cgggtaaaaa
     8161 tttgctcgct ctgatcacct gcctgcacca gtaggcctcg ttgcgttaac gccttttcct
     8221 tccagcgaca acgggctgaa aaccactcat acaaggcggc aatttgctgg gggagctccg
     8281 ggaaagcaga gtgctggcgg tgatccattt tttgcagcag cgccagatgc tgcgcgatct
     8341 gcgtcagcgc cagatgaccg ggatccagca tcgacgccag tttttccacc attgtcagtc
     8401 gcaggctaag cggcgcattt aacatgccgc cgttcgtcca tgtctgaagc tgcacacgcg
     8461 aaagaagttc ctgcatcagt cgttcacgaa actgctgctg atgggcttgt ggaaggcggg
     8521 catcatcgcc ctgcgccaga tccactaaaa agcggggata aaccgactcc agcacgcgac
     8581 cggggccgtc cagtaacgtc ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc
     8641 atcgcgtaac agctgggcgt cggtgtaaat ccagccgtga gtcatcacag tctgctgcaa
     8701 ttgctgctgc atcagcctga ccaccgattc attttgttga cgcagagcca ggctttcgcg
     8761 taaacgcgtc tgtaattccg tcaaacatga agcgaactca gcgcaaaaag tattcatgcc
     8821 tgccgtaaca gattcatcga cctgctctgc cagaacttta gccatttgtt ggcaataaag
     8881 atcgacttct gcgcttaaag ctcgttgcaa cacactgtaa tcaaccgtct ctgtcgggga
     8941 tttctcattt ccccgtcccc agtcgggctg attcaaccag cgcgaaaaag tctcacgcac
     9001 aacgcctaaa cgcgtgctct gctcgtccgt tgcatcctgg cgcgaaatga cttcactgaa
     9061 cagctggcga gtgttgacgt ggggaactac tccgtgaaaa acaggaaaat gaaccccagg
     9121 acgaaaccct gactcgctca attccatttt gacttgttgc tcaatgggcc gaataacatc
     9181 ggttaacacc cggcaaaggg tggattcaag ctcggcaaaa cgcagcgtaa agtcgcgact
     9241 gatggtgttc tgcgccgtct gtaacagtgt ctcacagcag gtacgaatct cgcttaacgg
     9301 ctccgaatca tcctgaaaca aggcggctaa ctgcgcattc agcgcatctt gctgttgacg
     9361 cagaaagtgg ttggcggagg tcagggccag ctcgatttca tgtttaatct cgccgctcac
     9421 ctgcgcctga ttgagttgca atagctgcaa actttcttcg acctgatgga tattttgccg
     9481 caattgttca caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg
     9541 ctgcgcgtaa ttcaacagtt tatgcgcagc agatcgcaga gcatacaacg aggcgttagc
     9601 gtaagcggca tgaagcaacg cctgaattgg ctgggcgaac agcgaatctt cccacaactg
     9661 atcggcagca tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc
     9721 gagcgcggca tgggcaaaat cttccaccca gcgttgttgc tctggcgctg gtaacttacc
     9781 gttgttggct aactcatggc gcgctcgatt tgccaagtag ccccacatcg acgacaccgg
     9841 aaatatctgc tgtggcgtaa tacagccttt catcagcgtc ccggaaatca gtgcccgcac
     9901 ctggtcggcg tcgtcactgt tacgatcctg ttgatcgaac ttattgacca gcacatacag
     9961 tggcaccgat tgccccaccg ccaaaatcgc ctcacggacc tcttcatcgg agatcgattt
    10021 cagttgcgta taatccagca ccgccagtac cgccgaggcg cgtgccagct gctggttaag
    10081 cattttttgc agatgcggtt gcccggcttc atttggcccg ggggtatcca gtaacgtcaa
    10141 ctgaccggga taactctcca gccccgccag atggacaaac tccacttcaa tcacgggaat
    10201 atgctcaatg gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg
    10261 cactaaatca ttcaaacttt tcagacaatg aaaaataggc tgggcaccca gataatattt
    10321 ttcgaaagcg acgccatttt cgatccgctg cataagcgca cgcatatctt tatctatttc
    10381 cagcacatcg gtcagatgct taatatcgca atcacgcagg cgctgttgta attgttgaat
    10441 taaacaatcg attggcgcga catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt
    10501 atggcgaata agcgtcggca gcgcagtcat tgggcgatta cgattaggca gaacctccgt
    10561 accaacaatg gcattaatgg tggttgattt ccctgctttc atagtaccga caatcgccag
    10621 caccatttcc agtcgggaaa ttttactcaa ctcattattc agcatcgcgt gacgttcggc
    10681 gatattaggc tgactccagg gtaaagccag ttgtggcgcg tcgtctccgg gtacagagag
    10741 aggcattttt tccagtaact gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc
    10801 ctgacttaac tcatacaggg tctgtgtgta catagaaaat tcttccttaa agcaaatttt
    10861 gttactttta tttagccaga ttgtttttga gttctgtttg agttctgttt ttggctttta
    10921 taattactgc aagaaataat tttatattta gtgtgttgtt ttttatcaga ataaataacg
    10981 tcttct
//
LOCUS       DAZPAC010000135        10909 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZPAC010000135 DAZPAC010000000
VERSION     DAZPAC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10909
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.135"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..169
                     /locus_tag="ACHUPM_002934"
     CDS             <1..169
                     /locus_tag="ACHUPM_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870654.1"
                     /translation="ARMIFAYINDKSIEILLLSCFAASDQKAIQRYIPQSQDGEDFRS
                     HLQYKLPLSAH"
     gene            380..703
                     /locus_tag="ACHUPM_002935"
     CDS             380..703
                     /locus_tag="ACHUPM_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR0870655.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            700..1530
                     /gene="amiD"
                     /locus_tag="ACHUPM_002936"
     CDS             700..1530
                     /gene="amiD"
                     /locus_tag="ACHUPM_002936"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR0870656.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1527..2540)
                     /locus_tag="ACHUPM_002937"
     CDS             complement(1527..2540)
                     /locus_tag="ACHUPM_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR0870657.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2639..4069)
                     /locus_tag="ACHUPM_002938"
     CDS             complement(2639..4069)
                     /locus_tag="ACHUPM_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR0870658.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4080..5081)
                     /gene="ltaE"
                     /locus_tag="ACHUPM_002939"
     CDS             complement(4080..5081)
                     /gene="ltaE"
                     /locus_tag="ACHUPM_002939"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR0870659.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5118..6836)
                     /gene="poxB"
                     /locus_tag="ACHUPM_002940"
     CDS             complement(5118..6836)
                     /gene="poxB"
                     /locus_tag="ACHUPM_002940"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR0870660.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(6969..7937)
                     /gene="hcr"
                     /locus_tag="ACHUPM_002941"
     CDS             complement(6969..7937)
                     /gene="hcr"
                     /locus_tag="ACHUPM_002941"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR0870661.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(7949..9601)
                     /gene="hcp"
                     /locus_tag="ACHUPM_002942"
                     /gene_synonym="priS"
     CDS             complement(7949..9601)
                     /gene="hcp"
                     /locus_tag="ACHUPM_002942"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR0870662.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9745..10644)
                     /gene="lysO"
                     /locus_tag="ACHUPM_002943"
     CDS             complement(9745..10644)
                     /gene="lysO"
                     /locus_tag="ACHUPM_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR0870663.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         2637 a         2897 c         2898 g         2477 t
ORIGIN      
        1 tgcaagaatg atcttcgctt atattaatga caaaagcatt gagatcttat tactttcttg
       61 ttttgcggcg tctgaccaaa aagcaatcca gaggtatata cctcagtcac aagatgggga
      121 agactttagg agtcatttac aatataagtt gcctctttcc gctcattaat gttgttggtg
      181 caacataata agacaggtaa gcggtaccct ataatgaaac caatatcggg ttatgcaacg
      241 gataatcgta aagacttaag tgggtgtttt tgacacaatg agatgatttc ctaaactgat
      301 caaaaccttc ttcccaacat ggcatatcag ttcgataatg ctttagagat cgaaggctta
      361 acaaacaagg agatcgatca tgcaattttc tacaacccca actctggaag gccagaccat
      421 cgttgaatat tgcggtgtgg tgaccggcga agcgatttta ggtgccaata ttttccgtga
      481 tttctttgcc ggtatccgcg atatcgttgg cggacgttcc ggtgcgtatg aaaaagaact
      541 gcgtaaagca cgggagatcg cctttgagga attaggctcc caggcgcggg cgctgggggc
      601 cgatgccgtc gtcggtattg atatcgacta cgaaacggtc gggcaaaacg gcagtatgct
      661 gatggttagc gtcagcggta cggcggtgaa aacgcgtcga tgagaagagt cttctggctg
      721 gtcgctgccg ctctgttatt ggcagggtgt gcaggcgaaa aaggcattgt cgagaaagag
      781 ggatatcagc ttgatacccg acgccaggcg caggcggcgt atccgcgcat taaagtgctg
      841 gtgatccact acaccgcaga tgattttgat agctcgctgg cgacactgac cgataagcag
      901 gtcagctcgc attatctggt ccctgcggta ccaccgcgat acaacggtaa accgcgcatc
      961 tggcaactgg tgccggaaca agaactggcc tggcatgcgg ggattagcgc ctggcgcggg
     1021 gcaacgcgcc ttaacgacac ctctattggc attgagctgg aaaaccgtgg ctggcaaaaa
     1081 tcggccggag tgaaatattt tgccccgttt gaaccgacac agattcaggc gcttattcca
     1141 ctggcgaaag atattattgc ccgttatcac atcaagccgg aaaacgtagt ggcacatgcg
     1201 gatatcgcac cgcagcgcaa agacgatccg gggccattat ttccctggca gcaactggcg
     1261 cagcagggga ttggtgcctg gccggatgcg cagcgggtta acttttacct tgccgggcgc
     1321 gcgccgcaca caccggtaga taccgcgtca ttgctggagc ttttggcgcg ctacggttat
     1381 gacgttaaac ctgatatgac accgcgcgag cagcggcgcg tgattatggc attccagatg
     1441 catttccgcc cgacgttata taacggcgaa gcggatgcag aaactcaggc gattgccgaa
     1501 gcattgctgg agaaatacgg gcaggattag cgcggcagtt ttccgtggtc gcgtagccag
     1561 gcggcagttt tctcgatacc ttcatctagg gtgatgatcg gctgataacc taactcttcc
     1621 tgcgcacgcg taatatccag cgtaaagtca aaattcaact tggagacgcc gtagtgggtc
     1681 agcggcggct cttttgctga cttgcggcct aaacgctcca tgctgcgggc gatcatatcc
     1741 agcatcgggt aggggacgga acgaatacga cagtcaatat tcaactcgtc gatcagcttc
     1801 tgcacgatgc tgcgcagtgt gcgatgctcg ccgttggtga tattgtacac acgcccggaa
     1861 ggtagcttat cgcaggcttc ctggcttgcc agccacattg cgtgcacggt attttcatag
     1921 taggtcatat cgaccagcgc actgccgcca tgcggtaaca gaatactgcc gtagtggtgc
     1981 atcatatgcg ccagacgggg aataaagact ttatcgtgcg gtccgaacag actttgtggg
     2041 cgcagaatag taaagcgcgt ttgtggattc gcctgcgaaa gcatattgat cacttcttcg
     2101 ctggctgctt tgctgcgggc aaactcgttg gcgaagcggt gagggcgaaa atcttcttta
     2161 atatcgcgat ggtggtgata atcgaagtac agggaggggg aagagatatg aataaagtta
     2221 cgcacacccc aggcgacagc ccattcaccc aggcggcgag tggcgcgaac gttagccaga
     2281 tcgaaagcct gttgtgtccc ccagggtgag gtaaagctgg agcagtgcca cagcgtatca
     2341 atgcccgcga gcatcacttt agcttgtgat gaaaccagct cggtcagatc cgccggaaca
     2401 aactctgcgc ccattttttc cagcaatttg cccattgcct cgttgcgacc ggtcgctcgc
     2461 acgctgatgc ctttctggca taaaaactct accgcgtttc gacctaagcc gctggtggcg
     2521 ccggtaacca gtaccttcat atcaatccac tgttgttgag aaaatgacgt gcgcattctt
     2581 ccgtgatttc ccccatgatg caatgggaaa catgaaagaa taacgcaggt tttgtcgatt
     2641 aatctgtgct ttgttctgcc agtctggcga tttgttttgc cattccgcga aaaataaaca
     2701 gatgcgcggg gatcatcaat aaccagtaaa acagccccgg cataccgtgc ggatgccaga
     2761 aagcgcggac atcgatagta cgatagtcgc ctttatcttc caggctaaaa cacagtcgtc
     2821 ccagccccga cgctttcatg ccaaataaca acgtaagttg tttttccggt tcaacgacaa
     2881 tcactttcca gctatccacc gcatcgccag tctgtaaata ttcgcgctcc gggcggcctt
     2941 tcgccagctt atgaccgatc gcgcggtcca tcaacgcccg tgtctgccac aaaatattgc
     3001 caaagaaata acgctcttta ccgccgattt ggttcactac ctgccataaa gcagcaaggc
     3061 tggcggacgt tttaacggta aaccccgcct gtttggcaaa ataaccgtac tccggtcgcc
     3121 agcgggcaaa ggcctgagcg tcgtagcccc agtcgctgga gttgaccagt ttttcctcct
     3181 ctttcaacgt gctacgtacc gcgtcatcga aagcgatcag ccgttgtggg atgagtgcac
     3241 gtagcgcggt atcatccgcc agcagatcgt gtttcagccc ctgaatcaac gccctggcgg
     3301 tggtgggcgg tacggaagta atcacattga gaaaccacac cgaaatccag cgggtgggga
     3361 gggggatggg gatcaaccag cggcgcttac cgctcaccgc cataaaatgt tcaaactgtt
     3421 gctgataact gagcacctct ggtccggcgg cttcgaagat gcggtgttcg ctggccggat
     3481 gatctaacaa cgccaccaga tagtgcagca agttttccag cgcgatgggc gtggtgcgtg
     3541 aacgtaccca gcgtggcggc gttaacactg gcaggttgta gaccatatcg cgcatgactt
     3601 cgaacgccgc tgaacctgcg ccaacgataa ttccggcccg aagttcggtc acaggtacat
     3661 tcgcttcacg aagaatgtcc gccgtagcct gacgagcacg cagatgatcc gactgctcat
     3721 gtggcggggc ctgcaacgaa ctgagaaaga ttaattgctt aactggtact tcacgtagcg
     3781 catcgcggac gttgagagcc acctggcgct cctgagcgat aaaatcgccg ccttcgccca
     3841 tgctgtgcac cagaaaatag acggtatcga tatcctgcaa cagggccgga aggttatccg
     3901 gccagctgag atcgacttta tggcaactga cgtttgccag ttgcagcttt gcaagcctgt
     3961 cgacatgacg tgccgccgcc aggatctgat gcccttgctg gctgagtgtg cgcaccagat
     4021 gctgaccaat gtagccactg gcaccgagaa ctaaaatgcg ttgcggcacg tctctctcct
     4081 taacgcgcca ggaatgcgcg ccagtgggcg gcgacttccg ccagttgttc gcgcgagacg
     4141 tcaagatgcg tcaccaggcg gacaatcggc gaggcgttaa tcagcacgtt tctcgctttc
     4201 atgtattcgc ctaacgcggc agcattttct tccccgacgc gaacaaacag catattagtg
     4261 tcctgacgca tcacatccgc gcctgcttca cgcagttgct ccgccatcca ggcggcgttg
     4321 tcgtgatctt cctgcaaccg tgcgacgtta tttttcaggg catacatccc ggcggcagcc
     4381 agaatcccgg actgacgcat cccgccaccg gccattttcc gccagcggat ggcacgttta
     4441 atgtaatcac gattaccgac gagtaatgaa ccgactggcg tcccaagacc tttcgacagg
     4501 caaatggtga acgaatcaca atattgcgtg atctctttca gttcgcagcc gtaagccacc
     4561 acggcattaa agatgcgcgc accgtcaaca tgcagcgcca gattgcgctt gcgggtaaat
     4621 tcccatgctt ctttcaggta ttcacgcggc agcactttgc cgttgtgggt gttttctaga
     4681 ctgagtaatt tggtgcgggc gaaatggata tcgtcgggtt tgattttcat cgccacttta
     4741 tccagcggta gcgtgccgtc ggcagccgcg tcgatgggtt gcggttgaat actgcccagc
     4801 accgctgcgc caccggcttc aaacagataa ttatgcgcgg cctgaccgac aatatactct
     4861 tcgccgcgtt cgcagtgact gagcagagcg accaggttag cctgggtgcc ggtcggcaga
     4921 aaaatggcgg cttctttacc ggaaagctct gcggcgtagt cctgcagagc attaacggta
     4981 gggtcgtctc cgtaaacgtc gtccccaacc ggggcggcca tcattgcttc gagcatggcg
     5041 cggctcggtc gggtaacggt atcactgcgt aaatcaatca tggcatgtcc ttattatgac
     5101 gggaaatgcc acccttttta ccttagccag ttcgttttcg ccagttcgat cacttcatcg
     5161 ccgcgtccgc tgatgattgc gcgcaacata tacaagctaa atcctttggc ctgttcgagt
     5221 ttgatctgcg gtggaatggc taactcttct ttggcgacca ccacatccac cagcaccgga
     5281 ccgtcgatgg agaaggcgcg ttgcagggct tcatcaactt cagacgcttt ttctacacgg
     5341 atacccgtaa tgccgcacgc ttcggcaatg cgggcaaagt ttgtgtcgtg tagttcggtg
     5401 ccgtcagtca aatagccacc agctttcatc tccatcgcca caaagcccag cacgctgttg
     5461 ttaaagacga caattttcac tggcagtttc atctgcacta ctgagaggaa atcgcccatc
     5521 aacatgctaa aaccgccatc gccgcacatg gcgaccacct gacgttctgg ctctgtcgcc
     5581 tgcgcaccca gcgcctgcgg catggcgtta gccatcgaac cgtggttaaa cgaacctaac
     5641 aggcgacgct tgccgttcat ttttagataa cgtgccgccc acaccgttgg cgtaccaacg
     5701 tcacaggtga aaatagcgtc atcggcggca aaatgactaa tttgctgcgc cagatattgc
     5761 gggtgaatgg ctttctcgct cggtttagct aaatcgtcca gccctttgcg ggcgtcgcgg
     5821 taatcttcca gcgctttatc cagaaacttg cgatcggctt tttcttccac caatggaagc
     5881 aatgcacgca gagtcgactt gatatcgccg accagtgcca tatccacctt gctgtgagcg
     5941 ccgatgctgg ctgggttgat atcaatctga atgattttgg catcggtcgg gtagaaggcg
     6001 cggtagggaa attgcgtgcc gagtagcact aacgtgtcgg cgttcatcat ggtatggaaa
     6061 cctgacgaga agccgattaa cccggtcatt ccaacatcat acggattatc gtattcgaca
     6121 tgttctttac cgcgcagggc atgaacaata ggcgctttaa ttttcccggc aaactcaact
     6181 aactctttat gcgcccccgc gcagccgctg ccacacatca gggcgatatt gctggaataa
     6241 cgcagcagtt gcgccagttt gcgtaactct tcttcttccg gcgtcacgac tggttgtggc
     6301 gcatgatacc agtgcgtggt tgccccttct ggcgcaggtt ttaacgccac gtcgcctggt
     6361 aacacgacaa ccgaaacgcc acggttaagc accgctttgc gcatggcaat cgccagtact
     6421 tgtgggatct gctccgggct ggaaaccagc tcgcaatagt gactacattc gcggaatagc
     6481 tcttgtgggt gggtttcctg gaaatagccg ctgccaattt cgctggaggg aatatgagcg
     6541 gcaatcgcca gtaccggaac gtgattgcgg tggcaatcga acaggccgtt gattaagtgc
     6601 aggttgccgg ggccgcacga tccggcgcag accgccagtt ctccgctaag ttgtgcttca
     6661 gcgccagcgg caaaggccgc cacttcttcg tggcgggtgg acatccactc gatggtgccc
     6721 atgcgattaa gactgtcact aagaccgttc agagagtcgc ctgtgactcc ccagatgcgt
     6781 ttcacccctg ccgattcgag tgttttggcg atataagctg caaccgtttg tttcatggtt
     6841 ctccatctcc tgaatgtgat aacggtaaca agtttagttc atctgacgga gggggaaggg
     6901 atgggagaga aaggaggcac taacggttaa atagcccgat gaaaggaata tcatcgggca
     6961 taaggcgatt atgcgagaac caaatccccc tgcggatggc aggagcaggc cagtacgtaa
     7021 ccttcagcga tttcggcgtc ggtcagcgtc attgtactgc tcaccgtata ttcaccggaa
     7081 accacttttg tcttacagca gccgcaaaca cccgcacggc aggcagcgac aaccggaacg
     7141 ttattgcttt ccagcgcctc cagtagcgtg gtgccaaccg gggcgtaaaa ttctcgtgcc
     7201 ggttgcagtt tggtgaattt cagaccgctg gtcgccgctt ctgctactgg agtgaagaat
     7261 ttctctttaa agaaacgcgt cacgccgagc gctttcactt cctgctctac ccaatccata
     7321 tacggagccg ggccgcaggt catcacggta cgtgaagcta agtcaggtac acctgccagc
     7381 agttcgcgag tgagacgacc agcgataaag ccttcggtaa cgttattttc tgccaccagc
     7441 gttaccggat agttacgcca ctcatcggcg aaaataacat cctgcggcgt acgcacgttg
     7501 tagatcaccc gcacatcggc ctgtggacgg ttcttcgcaa gccagcgacg catcgacata
     7561 atcggcgtga cgccgcagcc tgccgccagc aacaggaatt tatcttctgc tttatcgtcg
     7621 caggtaaatt cccccatcgc gtccgaaagc cagagataat caccgcgttt tacatcgcgc
     7681 gtcagccact gggagccgac accgtcatca atccgccgca cggtcagggt gatatattca
     7741 ctcacgcctg gcgtggagga aatggtgtaa gcacgcagcg tttccgctga gtttcgcacg
     7801 ctgaccagtg catattgccc ggcgcgatat gggtagtaat cgtggcaaat cagggaaatc
     7861 gtccacacat ccggcgtttc ttgcgtaatg tgatgaacct gcatccgcca cgggcattga
     7921 ttcgttggca tcgtcatcga caaactcctt acgcgctcaa cagttgcttc atgtcttctt
     7981 caacagtggt gatagaacgc aggccgaatt tctcgttcag caccgccagc aggtctggtg
     8041 tcaggaaacc aggtgcagtc gggccggtga cgatattttt cacgcccaga gaaagcagcg
     8101 tcagcagaat gacgatcgct ttctgttcaa accaggagag caccagcgac agcggcagat
     8161 cgttcacacc acagcccagt ttctctgcca gagtgacagc cagaataatc gctgagtaag
     8221 catcgttaca ttgacctgca tctaccagac gcggcagacc ttcgatatcg ccaaactcaa
     8281 gtttgttaaa gcgatattta ccacaggcga gggtcaggat caggcagtca tccggcacgc
     8341 tggtggcgaa atcggtgaag tagtggcgct cgccgcgtgc gccgtcacag ccaccaagca
     8401 ggaagatatg acgcagtttt tcacggctca ccagatcaat cagcgtatca gcagcgccaa
     8461 gcagcgtctg gcgaccaaaa cccacggtga taaggtgcgg aatttcgctg tacgggaagc
     8521 ctgccatctg ttgcgcctgg gtgataaccg cagagaaatc atcaccgtcc agatgacgca
     8581 cgccaggcca gccaacaatg ctgcgggtcc agatacgatc gtcataagcg cctacggttg
     8641 ggtcgatgat gcagttcgag gtcatcacga tggggccagg gaaacgagcg aactccactt
     8701 gctgattctg ccagccgctg ccgtagttac cgaccagatg cttgaattta cgcagctccg
     8761 ggtagccatg cgcaggcagc atttcgccgt gggtgtagac attaacgccc gtgccttcgg
     8821 tctgttccag caggttgtag agatctttga gatcgtgacc ggaaatcaga atgcatttac
     8881 ccgccgtcgc tttgacgttg acctgggttg gcgtcgggtg accgtattta ccggtttcgc
     8941 ctgcatccag aatgctcatc actttgaagt tcatctggcc gatttccatt gaacactcaa
     9001 gaagcgcgtt catatcggca ggccaggtcc ccagccacgc catgatttta tggtactggg
     9061 cataaatatc gttgtcgtat tgaccgagaa catgcgcgtg ttccatatag gccgccgcac
     9121 ctttcaggcc atacaggcac agcagacgca ggccgagaat gttttcgcca atcgccgctt
     9181 tatctttgtt aggggtaaat tctgctgcct gacgttgcag ctcgccgaga tcatcgctca
     9241 ccagttgcag gtcagccatc gggttatcga cgcgcgcgtt ggcatctaca gccaggcatt
     9301 gtgctttcag cgcctcgcgc aaggcaatcg cttcacgagc gtagccgaca atacgcggag
     9361 aatcgaagtt aacgttggtc agggttgaga aaaaggcacg tggcgcgaag ctgtcaacat
     9421 cgtggttgat gatgccgtat tcacacgctt ttaccgccca ggcagaaagc ccttgcagcg
     9481 ccgcgatgag taaatcctga aggtcagaag tttccgccgt tttaccacac atcccctgcg
     9541 cgtatgagca gccgtttcct gccggagtac ggatagtttg ttcacattgc acacaaaaca
     9601 tgatcacacc ttttaaagtt atatttaata tacatgttta aggttaagac gcttaacgcg
     9661 gggataaaag ggatttttca tgcaacttta agggagattg atttagcgca attttggcgg
     9721 cagggatcta ccgccagaga ggtattacgc agagaaaaag gcgatgagga tcggcactaa
     9781 caggctaaga ataaaaccgt gaacaattgc cgccgggacc atatccagcc cgccagtacg
     9841 ttgaagaacg ggcagggtga aatccattga tgtggcaccg cataagccca gtgcagtaga
     9901 gcggctgcgg cgaatcagcc cagggatcaa cataatagca atcagttcac gggccagatc
     9961 attaaaaaac gccgcgctcc cgattaccgg accaaaagat tcggtcaata aaataccgga
    10021 aagagaatac cagccgaaac cggaggccat tgccagcgcg gtattgatgg ggagatcaag
    10081 aataaaggcg ttaattaaac caccaattaa tgaactgaca accaccacca cggcgacaat
    10141 cattccccgg cgattaagga caatctgctt taaggtcatg ccattattgc gcaactgaat
    10201 accaacgagg aaaagtagca aaattaacgt gtattcactg gcttcggtcg cgtgttgtaa
    10261 gaaagccagt ccactcagac caatggcaaa accaatcact actacgccgc acagttttag
    10321 cgactccagc gccatcgcaa tacgcgacgg gagtttttct tgctgatggt ggttgcgcca
    10381 cggcaggcct cgctccagcc acatcagggc ggcaatatta cacagtaaaa taacggtaat
    10441 actgacggca gaataatgca gaatcgccaa caggttactg gcgaggttat cgagaaacgc
    10501 cagactgata cccataaaaa agagaataag gtaaaccatc cagcttaata gctgattaat
    10561 aacttttaac gcagcttgtt ggcgaagcgg aatgaggtaa cctacaatca ggggaaccag
    10621 aatgattaac agcccagaaa acatgaaaac ccagtccttg caaagatgaa gtcgaaatgc
    10681 gcgatgacac actactgaaa gcggaaggac gagtaaagtt gcaattaaaa ggaaatgtta
    10741 tgcataagga gcagtagagt attcgttttc atttaaagat attcttgcgc tttaattaca
    10801 aactgcaccg atgttggtgg cgtcaaaatc gccgaggcgt tccctgaagg ccggggcagc
    10861 ccacatggat gtgggctgag ggcgcgtttt acagggatgt tacctcgcg
//
LOCUS       DAZPAC010000136        10907 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZPAC010000136 DAZPAC010000000
VERSION     DAZPAC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10907
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.136"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..510
                     /gene="csgD"
                     /locus_tag="ACHUPM_002944"
     CDS             <1..510
                     /gene="csgD"
                     /locus_tag="ACHUPM_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR0870664.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
     gene            515..904
                     /gene="csgE"
                     /locus_tag="ACHUPM_002945"
     CDS             515..904
                     /gene="csgE"
                     /locus_tag="ACHUPM_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR0870665.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            929..1345
                     /gene="csgF"
                     /locus_tag="ACHUPM_002946"
     CDS             929..1345
                     /gene="csgF"
                     /locus_tag="ACHUPM_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR0870666.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1372..2205
                     /gene="csgG"
                     /locus_tag="ACHUPM_002947"
     CDS             1372..2205
                     /gene="csgG"
                     /locus_tag="ACHUPM_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFR0870667.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2270..2761)
                     /locus_tag="ACHUPM_002948"
     CDS             complement(2270..2761)
                     /locus_tag="ACHUPM_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR0870668.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="ACHUPM_002949"
     CDS             complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="ACHUPM_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR0870669.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="ACHUPM_002950"
     CDS             complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="ACHUPM_002950"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR0870670.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="ACHUPM_002951"
     CDS             complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="ACHUPM_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFR0870671.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5405..5492
                     /locus_tag="ACHUPM_002952"
     tRNA            5405..5492
                     /locus_tag="ACHUPM_002952"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5439..5441,aa:Ser,seq:gga)
     gene            5537..5673
                     /locus_tag="ACHUPM_002953"
     ncRNA           5537..5673
                     /ncRNA_class="other"
                     /locus_tag="ACHUPM_002953"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5764..6450)
                     /locus_tag="ACHUPM_002954"
     CDS             complement(5764..6450)
                     /locus_tag="ACHUPM_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR0870672.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHEWADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="ACHUPM_002955"
     CDS             complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="ACHUPM_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR0870673.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="ACHUPM_002956"
     CDS             complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="ACHUPM_002956"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR0870674.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="ACHUPM_002957"
     CDS             complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="ACHUPM_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR0870675.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
BASE COUNT         2847 a         2807 c         2508 g         2745 t
ORIGIN      
        1 gacgatatat cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
       61 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      121 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      181 ttttatgcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
      241 gaatgctact ttacgcaaaa gcttgccagc tacttgatta cgcattcagg taactatcgt
      301 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataaactg
      361 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
      421 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
      481 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
      541 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
      601 attgctaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
      661 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
      721 gggaagctgg atcactataa cggtcaatca ggacgttatt ttccagactt ttttatttcc
      781 gttgaaaaga gacttcgaga aaactgtcgt ctttgcactg attcaaactg aagaagcact
      841 aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt
      901 ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
      961 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
     1021 tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc aaaactctta
     1081 taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt
     1141 tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa
     1201 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
     1261 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
     1321 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
     1381 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
     1441 gccgccagac cgacattaat gcctcgagct cagagctaca aagatttgac ccatctgcca
     1501 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
     1561 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg
     1621 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgtcagag tttacaaaac
     1681 ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa acggcacagt tgccattaat
     1741 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatca tggttgaagg ttcgattatc
     1801 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     1861 gacacgcaat accagctcga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc
     1921 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     1981 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     2041 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     2101 aatgatggta tcgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaatgac
     2161 attctggtta aataccgcca tatgtcggtt ccaccagaat cctgacagat aaaaatgccg
     2221 gaattaataa tccggcattt tactgtttaa gcgaaagcgt gagtgcccct cacgctttat
     2281 ttttgatttc ctgctcacgg tgagccgtct ttgcactacg cgccgccagc cacaggccgc
     2341 tgtttttcat tgcgtaacca aataccagcc caagcgccag cgaaggtaac accagtttcc
     2401 agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga acaaatgaaa
     2461 gcagcagctg tttggcctga atacacatca gaaacgccac aataccggtt atgacataac
     2521 cgagaatttc cagatgtggt gccagtgcac taccgtaaat aatgaccatc gcccacacca
     2581 cgccactaag cagggttgca gcggagatcg ccagcccttt cagcccacct tgcgggcagg
     2641 caaagtaggc cgtacagccc aagaagcccg cccagcttag taagcccagg gaaacagcca
     2701 cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga agtatgttca
     2761 tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca ttttatgcaa
     2821 caacaaatta tctgttgcag ttatgagtga cgatgatcac atttactctt cagaatcttc
     2881 ttccagctcg tcccacattg cactaatggc atcacgggtt agcggtgcca tggtgcgcca
     2941 gaaaggcgtg gttgcatggg cctccacttt gccgaggaac gcaccacacc agggtaacag
     3001 atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact gatcttccag
     3061 ccaggaagcc gcaagcagca atgtgccgat gtgatcggct ggcgtatccg ccagtggcat
     3121 ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt acgtcctcaa cccatgcgct
     3181 acgatatggc ggcactgcac attcatcgcc gataaacaac gcattgtaat cggcagagac
     3241 ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac tcatcctgct ccagtggcca
     3301 gttcgcagcc agtttcccct cacgaatcag ggtaaacagc ggcaccagta aaggatcttg
     3361 cggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact cgttcattaa
     3421 tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc cataccgcga
     3481 gattcaagga agttcagtaa gcggcgcgga gaaacattca aaatgcgctc tggcggaaaa
     3541 tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa cgcggtgtga
     3601 gaatccgagc ctaatgccac ccaacctcca gcatcacgta ccgctgcggc taccgcacga
     3661 cagttgtctt cactgccctt acgtgagtgt aaaaatgaag aattattgat ttccagcgcc
     3721 acctgatgtt tcgcggctgc ttcagcaacg gctttcacat ctatttcata tttgggattt
     3781 ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat cgcttgtgta
     3841 ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa aaccggcaat aatcagatcc
     3901 agcgagtcaa acattttacc gctgcaatca atttcaccat caacattttt aatgttagct
     3961 tcgatgccgc gcaggatccc taccccatca accactcgcg gccagatacg catgttaatg
     4021 aagtgccagt gatgcggagc atcttccata tccgggccat gatcggtgat cgcaaaaagt
     4081 ttaatgccct tttgtttggc ctgggcaatg taatcactca atgtgctata tgcatgtgtg
     4141 ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg tattcaattc
     4201 ccgccaagga tagcaggaat cctgatgctt tattagtagc cgcgtgcgcg gtcgacttgc
     4261 ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc gagaaatgta ctccacagct
     4321 tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag
     4381 agcggacttt caggcggtaa gggttcacga ttaaaaacat ccaacattgc gcctttaact
     4441 ttgccgctat ccagcgccgc gagcaggtca tcttccacaa catgaacacc acgcgccagg
     4501 ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat aatgccgacg
     4561 gtttcagggg tattcggtaa caaattaatc aatacccgac attggctcag aaatgcagac
     4621 agttcttccc gtccggcaaa gctttgcacg ccaggccacg atttacgggt tcgactccag
     4681 caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa ctttactgcc cagtacgcct
     4741 gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc
     4801 gaactatttt gctggatgcg ataatcgtca aaacgtcgaa accaatgcag cacctgactg
     4861 acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca
     4921 gaagggttca gcatttcagg gtgtgcctgt agcttgctca aaatagaatc aacaccggcc
     4981 ccgagtgcga acaccgcttt aagatcgcgc cctgccagca tttcaacagg aggatgccag
     5041 actaaagcat aatcagcaga gtcattatct ccgcttttcc atgctctgac tcttgcctga
     5101 ggaatagctt tgcgcagtgc ctcaatccac cattgggtat cgaacgttgg gtgataaaag
     5161 atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat ttattcgcac ttatcgttat
     5221 gatctacaaa ggccaccagc ataacaaatc cgtggtcggt ggcaaaaaaa gcagatttcg
     5281 cttattaaaa ccacacattg attgaagttt gaataaacgc gcgatttttt caaaaagttt
     5341 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     5401 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg
     5461 tatcgggggt tcgaatcccc ccctcaccgc catatttaaa gaagagctcg tacgaaagta
     5521 cgggcttttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccggggttcg
     5581 acaactggcg acagccagtt ggacagaccg tgaacgcagt gagcgggctg cccgcagggc
     5641 gagcgaagcg agtcaatccc cccctcaccg ccatctttaa agaagagctc gtacgaaggt
     5701 acgggctttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccgggatact
     5761 tagttaatgc accggttcac tctttgtcgc cgcgactaat gggcgaattt tgtcacgagt
     5821 ctcccagacc ggaggaattt cctccaattt agtgacagaa gctcgtacaa aaggcacccg
     5881 tgcaagagtc ataattgtat ccaaccaacg gtcacgaata atgcgatccg cccattcatg
     5941 actcgggtca tccagctcaa taacacagac tattttattg ttcttctttg aaaccagaac
     6001 ataatccatt gccatgccat tataaattgc aaagaaacgc tttctttctt cttcgctaaa
     6061 ctcaggttgt tctggaaagt tgatcaagcg ccccagagag acctggcaat ggatatgaca
     6121 ataatgtcca aaccattttt ccagattatc gaaatacatt ttttcacaac gcgtgattaa
     6181 ccactctttt ctttccaata aaataccttc ggcaaccatt ccctgaaata actcatctcg
     6241 ctcttgccaa cgagagtcac gataggtttt ctgcggtata tagctccttg tcgacgcctt
     6301 agcggcaggg gaagttgcag atgaagggac gggttgcgat ttagcgatga ttaccgatga
     6361 ggcaactttt cggagtgtca attttctggc aacagcaaaa ccggcgataa atgcaattgt
     6421 caaacaagtt acaaaagcca taaagctcat aattaatttt cacctatcca tgttgattat
     6481 atttcattac caatgtaact cgctgcagcg tgttacatgc caacaatgca acgatcaata
     6541 tatagttagt atcatgtttt gattattaca accatcccta tttagccatt gcggcttgcc
     6601 ttccacaaaa aaataacaac acacaatatt agcaaagaat taattaagat taaccttaca
     6661 tttataatat tatccctaaa gaataaatca gaggaatata ttcggtgtta aacagtcagt
     6721 caaagtatag cccattgttg attaaacctc ataagcaaaa ttggcaccag atctcctgta
     6781 caggttgaat ggccgaaaaa agataggcag atgctcattt ttttggcttc tggaagaaaa
     6841 actctccgcg ctggcagtat tacactggtc tgatgctatg acgaaaaatg cctggtaaga
     6901 gtatctaaac cagactccag ttcagcttat caacacgctc aaagtcgccc ccctgaacgc
     6961 taacctgaag tgaaaatctg ttgaaataaa acaaagcccg gttcgcccgg gctctgcacc
     7021 gataacacac ttaactgtag gcatgcagcg tacgttggca aagtgccgaa cgtacgcagt
     7081 cctctttacc gaaccggacg atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt
     7141 cacttaatcc ggagcacacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga
     7201 taaccgtcac gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat
     7261 tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga
     7321 acggcgcaat ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc
     7381 ctaagcgccg gaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga
     7441 tatctccagg taagaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa
     7501 tcctgtcgac atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaagttt
     7561 ttccgccccc ggcttcgccc gtggcgaata tcagctgctt actctcaata gccttcagat
     7621 agtgcaattg cgcttcattt cgcgcgagga tgggcgaagt atcgcgactg tcgcgggcca
     7681 taccaatggc ttctacgccg cccatctgca caagcgaggt gaccgattct tcttcacgct
     7741 gcttatggct gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca
     7801 ctgctttttg tcttcccata gagagcacct tgagttgttt gtattcatca cacgcgccgt
     7861 tggcagcgcg attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg
     7921 cttttggata aaatgccgaa aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg
     7981 aaaagaaagg atgaggttga aaatgcaaag tgacgagatg actaccggag gagaaaactc
     8041 cgcgagtggt ggcgcgttga ttatctaaaa catgtccagt acaggacgtt accatccgcg
     8101 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     8161 agatctcgca tttattgcaa caatatattt acttatattt aactataaaa cactatttca
     8221 gtgacattag tttctactgg aaagatgaca gagtgatgac agtgatgaaa aaagctgtgt
     8281 gctttcagca ggatttgcat caggcatcag tgcacgatcg ccggatgcgg cgtgaacgcc
     8341 ttatccagcc taccaaaccc gcactgattg taggcatgat aagacgcgca agcgtcgcat
     8401 caggcatctg cgcacaaatg ccttatccgc ctaaaaacat taaacccgca ataacgcgct
     8461 tccgagataa tcgttcgcgt ccttcactcc cggcagcgaa aaaaaataac cgccgccgat
     8521 aggtttaacg tattcctcca gcgcttcgcc attgagcctt ttttgtactg tcaggaagcc
     8581 tttttccaga tcgtgttggt agcagacaaa cagcaacccc atatccagtt gtccggagtt
     8641 ggtgaccccc aacgaataac tgtagccgcg acgcagcatc aggctggact cactctccgc
     8701 cgtgcgggga ttcgccagcc ggatatggct gtccagcgca atcaccttcc cttccgggtc
     8761 gctggcgtaa tcaggcacat catgctcatg ctgcattccc agcggcgcac cggtttgctt
     8821 atcacggcca aaaatcgtct gctgttcttt cagcggcgtt ctgtcccaaa attccactcg
     8881 aaactgaatc aagcgtactg cctgatagct gccaccgatt gtccacgcag gctcctgctg
     8941 atctgccgtt acccacacca ctttttgcat caacttatca ttctggctat cgggattggc
     9001 agtgccgtct ttgaaaccca gcaaattaat cggcgtctct ttgcctttac tacgcgacac
     9061 gtgatcggaa ataaaccctt cccgcttcca gcgcacactg agcaaatccg gcgtgtgttt
     9121 gatgatatcg cgcagcgcat ggataaccgt gtcctgggtg ttggcgcaaa tctgtagcaa
     9181 cacatcacca tgacataacg ccgcatccag cgagtcgttg gggaaacgcg tcatcttctg
     9241 cagctttttt ggcatctgtg gcgcaaggcc aaagcgctca tcaaacaatg agtgacccac
     9301 cgataacgtg atggtgagat tatcgggcgc aatgtagccg ccaagaatgc cggaatcgag
     9361 tggtggcagg cgcggatttg gcgtttctgg tgctgctccg ccctgagtca gaaaagcaaa
     9421 acgctgagtc aacaagcgaa acaaccgctc aagatcggct ttatcgctgg caagcacatc
     9481 aaacgccacc agcatcattg cggcctgttg tggcgtcagg atccctgcct gatgctcacc
     9541 ataaaacggc tgtttctcat tgcgtgcatc cggtgaaagc gtgcccggtg cactttgcgt
     9601 tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc agcgcaccaa tccctttcag
     9661 taaacgtcgg cgtgacggtt cattcacgcc gttttcatct ttatactgca taacgcttaa
     9721 tccagtccca gcacaccgcg aagttgcgcc agatcttccg ccagcgcagt aatcggtcct
     9781 ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta
     9841 cggtatttcg ccagaatggt atcgaccttt ttaaagttgg catcgacttt tgccagcagt
     9901 tccgggttgg ctttttgcag ttgtggacgc agcaaatcga caattttctg cgagccttca
     9961 acgttagcct ggaaatccca cagatcggtg tggctgtagc gatcttcttc accgctaatt
    10021 ttgctggctg ccacttcctc aatcagtccg gctgcgccgc cgaccacttt tgaaggtggg
    10081 aaagccagtt cactgatgcg tttttgcaaa tcgaccacat ctgtataaag ctgctcagcg
    10141 tactgatcca tccctttggt ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa
    10201 ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat cttcacgggc gtcaatgctg
    10261 ccatccagat cggagaacag ttcagcaatc ggttcaatgc gctcatagtg ctggcgcgtc
    10321 ggcgcataca gtgctttcgc tttttcgata tcgcctgctt taatcgcgtc ggtaaaggct
    10381 ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat acgctttata tgcagtaatt
    10441 gcaccaccaa gacttaacag cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg
    10501 acgatcaact tccctttcgg gttagtcagc agaccgcagg tcatatcgta ttcgccaggc
    10561 tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga tattttcccg ctcttccacc
    10621 accatcacgc ctttgaggat ctcccactcc agcgccttct ggctatggtt ctgaataatg
    10681 aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt cgcactgctt atccgtcacg
    10741 gtcactttga cctgcggcac atcagcggcg ttagccataa aagcagaaga aaacagcgca
    10801 gccacgctca actgcaatgc gttacggcgg aagttaatgg tcatgacgag actatccctt
    10861 taaagtatgt tgtaactaag caagagtttg cgtcgtattt gttacgc
//
LOCUS       DAZPAC010000137        10887 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZPAC010000137 DAZPAC010000000
VERSION     DAZPAC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10887
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.137"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..2175)
                     /gene="katG"
                     /locus_tag="ACHUPM_002958"
     CDS             complement(<1..2175)
                     /gene="katG"
                     /locus_tag="ACHUPM_002958"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR0870676.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDL"
     gene            complement(2504..3394)
                     /gene="metF"
                     /locus_tag="ACHUPM_002959"
     CDS             complement(2504..3394)
                     /gene="metF"
                     /locus_tag="ACHUPM_002959"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR0870677.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            3470..3604
                     /locus_tag="ACHUPM_002960"
     CDS             3470..3604
                     /locus_tag="ACHUPM_002960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870678.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(3743..6175)
                     /gene="metL"
                     /locus_tag="ACHUPM_002961"
     CDS             complement(3743..6175)
                     /gene="metL"
                     /locus_tag="ACHUPM_002961"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR0870679.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6178..7338)
                     /gene="metB"
                     /locus_tag="ACHUPM_002962"
     CDS             complement(6178..7338)
                     /gene="metB"
                     /locus_tag="ACHUPM_002962"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR0870680.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            7615..7932
                     /gene="metJ"
                     /locus_tag="ACHUPM_002963"
     CDS             7615..7932
                     /gene="metJ"
                     /locus_tag="ACHUPM_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR0870681.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            8116..8724
                     /locus_tag="ACHUPM_002964"
     CDS             8116..8724
                     /locus_tag="ACHUPM_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR0870682.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            8869..9544
                     /locus_tag="ACHUPM_002965"
                     /pseudo
     CDS             8869..9544
                     /locus_tag="ACHUPM_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(9631..9953)
                     /locus_tag="ACHUPM_002966"
                     /pseudo
     CDS             complement(9631..9953)
                     /locus_tag="ACHUPM_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
     gene            complement(9955..>10887)
                     /locus_tag="ACHUPM_002967"
     CDS             complement(9955..>10887)
                     /locus_tag="ACHUPM_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0870683.1"
                     /translation="EAGLKRVVKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPD
                     VVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGNLLNEENPHQLQ
                     QLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYTYPLSPVNSMDP
                     LGLYEFKSKNIDDIGIFALAMCNGESINENKEYGGLICKKQGEYFPMNPISSNDNDSV
                     DLRNIKCPEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGK
                     KEYSSYLGTPNNTYLKYNPKAKGNGVTIIRQGSN"
BASE COUNT         2629 a         2987 c         2648 g         2623 t
ORIGIN      
        1 caggtcgaaa cggtcgaggt tcatcacttt cacccatgcc gccacgaagt ctttaacaaa
       61 cttctcgtga gcatcgctgc tggcgtaaac ttcagccacc gcacgcagga cagagttaga
      121 accaaatacc agatccgcac ggctggcagt gtatttcact tcgccggttt cgcggtcacg
      181 gccttcaaac agctctttcg attcgtcggt cgctttccac tcgtaacgca tatccagcaa
      241 gttcacgaag aagtcattgc tcaatacgcc aacgcggtca gtgaagacgc cgtttttgct
      301 gccatcgaag ttggcaccca gtacgcgcat cccgcccacc agcgcggtca tttccggcgc
      361 ggtcagggtc agttgctggg ctttgtcgat cagcagtgac tcggtggtgg aaacatccag
      421 acgagcgcga tagttacgga aaccgtcagc aattggctcc agcagctcga acatctcaat
      481 gtcagtctga tcctgacgcg catcaacgcg gcccggcgca aacggtacat gaatgctcaa
      541 acctgcggcg cttgcggctt tctcaacacc aaccacacca gccagcacga tgatatccgc
      601 cagcgaggct ttaccagact ctttctggat tttctccaga acaggcagag cacgaacggc
      661 tgcggcgttc acatcccagt cgcgctgcgg cattaatgcc agacgcgcac cgttggcacc
      721 accgcgtttg tcgccaccac ggaaggtaga agcagatgcc caggccaccg atacccgctc
      781 actaacagac agaccagaat ccgcaatcgc gaatttcaga tcgataatgt cctgctcggt
      841 cgggttgtag atcggctgcg gcagcggatc ttgccagatc agatcttctt tcggcacttc
      901 cggcccgatg tagcgagatt tcggccccat atccctgtgc gtcagtttga accaggcacg
      961 ggcaaaggct tcgttgaacg cctgcggatc gttgaggaaa cgacgagaga tcttctcgaa
     1021 ctcaggatca aaacgcagcg tcaggtcggt caccagcatc gtcggtttac gtttcttcga
     1081 cggatcgaat ggatccggga taatttccgg tgcgtctacc gcttcgaact ggattgcgcc
     1141 agccggactg cgggtctgta cccactcata tttgaacagg ttttcgaaga aatagttgct
     1201 ccactgggtc ggcgtctggg tccagacaac ttccagacca gaggtaatgg catctgcgcc
     1261 aacgccgctg ccgtaagtgc tcgcccagcc taaaccttgt tcttcaatcg gtgcagcttc
     1321 tggatcagga cctacgtttg atgtcggacc ggcaccgtgg gttttaccca gcgtatgacc
     1381 acccgcaatc agcgccacgg tttcttcgtc gttcatgccc atgttgccga aggtcgcgcg
     1441 gatagctgct gccgcagaaa gcggttcgcc gctgtgatcc gggccttccg ggttaacgta
     1501 aatcaggccc atctcggttg cacccagcgg tgctttcgcc agcgcttccg gatgacggtg
     1561 agtcagccag gctttttcat caccccagtt aacatccaga tccggttccc agacgtcttc
     1621 acgaccggca ccaaaaccga aggtacggaa gccggagttt tccagcgcca cgttacctgc
     1681 gaggataaac aggtcggccc aggagatttt ctgaccatat ttctgtttga ttggccacaa
     1741 caggcgacgc gctttatcga ggcttacgtt atccggccag gagttcagcg gtgcaaaacg
     1801 ttgctgacca cgacccgcgc cgccgcgtcc atcgattgaa cggtaagtac ctgcgccgtg
     1861 ccaggccata cgaataaaca gaccggcgta gctgccccag tcggctggcc accacggctg
     1921 agattctgtc agcagggctt tcagatcttt tttcaggccg tagtaatcta atttgctgaa
     1981 ttctttgcgg tagtcaaagt cctcacccag cgggttagaa cgattagaat gttggtttaa
     2041 caggtcaaca cggagttgat ttggccacca gtcgcgagtg gttgtgcccg cccccgcgct
     2101 ctggtcgtga ccgccctgat ggaacgggca tttgccagtg gctgtcgtgt tatggatatc
     2161 gtctgacgtg ctcatcaatg tgctcccctc tacagtgtta ccgttacgat acacagcgtt
     2221 agagagaagt tataattgaa ttaatccacg gatgcgatag ttgagatctt acatattgtt
     2281 ggttaaagag atgtaggtca aattgatcgt aattaagctt ttttgattat gtccgaattc
     2341 ggacaaatgc tttataaaaa agggttttag cttcatatcc ttcaagtaat gagggagaaa
     2401 acaaggctga caaccttatt ttcccgccct catttcgagg cagcattttg tgctctgttt
     2461 aaaatttgtg atcactgtgt gattttcaca aaagccacac tatttataaa ccaggtcgaa
     2521 cacccagcgt atggcaaatc gcgtaactca tttcagcgcg gttaagcgta tagaagtgga
     2581 aatctttcac tccttcacgg cttaaaatct tcaccatatc catggcaata ttcgcgccca
     2641 ccagtttgcg ggtttcggca tcatcatcca ggccgtcgaa catttgcgcc atccacgccg
     2701 gaatacgcac gttggtcata tcggcaaatt tcttcgcctg tttaaagtta gataccggca
     2761 aaattcccgg aataatttcc acatcaatgc ccgccgatac acagcggtca cgaaaacgca
     2821 ggtagctttc gacatcgaag aagaactgag taatcgcgcg gttggctccg gcatcgactt
     2881 tgcgtttcag attaagcaaa tccgcctgag cgctttttgc ttccgggtga acttccggat
     2941 acgccgccac ggagatatcg aaatctgcca cttcttttaa tagcgtcacc aggtcagaag
     3001 catacatttc tggcttacca cttcccggcg gcagatcgcc acgcagcgcc acgatatgac
     3061 gaataccgtt attccagtag tcgcgtgcaa tggtgcgcaa ctcgtcgggc gtcgcatcaa
     3121 tgcaagtaag atgcggtgcc gcttccagac cagtgcgatc tttaatgcct ttaataatgc
     3181 tgtgcgtacg gtcgcgctcg ccggagttcg cgccataggt caccgataca aacttcggtt
     3241 tcaggctgct aaggcgatcg atggagttcc acagggtctg ctccatttca ctggtacgcg
     3301 gcgggaaaaa ctcgaacgaa acgttaatct gcccctggac ttctgccagg ctctgattca
     3361 gggcatcccg ctggctggcg tgaaaaaagc tcatacctta cctcatcaat cgcttgtagt
     3421 tatatgttgt gttgtgcaca tctatccgtt tagacgtcca gatgtaaaga tgaaggaaaa
     3481 gccgaagggc gtcaaccgaa aaatgaccaa tgatggtgag gaaaaatcag ggaagatgaa
     3541 aaaacttcag gatggtaaga aaaagaaaat ccggtatgca aagcaaaccg gactgcaaat
     3601 ataaactaac catgtactta ttgcttcttg ccggattcgt cgtaaacgct taatccggcc
     3661 taatcctatg ataattcaat atatttcatt agaaaccata aacccgaaaa catgagtacc
     3721 gggcattatt aaatttctga aattacaaca actgtgccag ccggttgata tccgactgaa
     3781 tcgccccggc ggtgacgtcg cgcccagcgc caggcccacg gatcaccagc ggattatcgc
     3841 gataccagcg gctttcgatg gcaaagacgt tatcgcacgg cagcagtgat gccagcggat
     3901 ggtcttcacg caccgcttcc acgcccacac gcgctttgcc attggcatcg aaacgcgcca
     3961 cgtagcgcag caccagcccc atttcgcggg ccgcttccag ccgttgcacc atctgctcgt
     4021 taagttcatc gccattttca aagaaatggt cgatgctgcc gccttcgcaa tgggcaggca
     4081 ccagcgactc cacgcgtacc tgatccggtt cgatgttgta acctgcttca cgcgccagaa
     4141 tcaccagctt gcgcatcacg tctttgccag agagatcgtc acgcgggtca ggttcggtta
     4201 agccctgctg ccacgcctga tccaccagct cggtaaacgg cacgctaccg tcgaattgca
     4261 ggaacaacca ggagagcgtg ccggagaaga tcccgctgat cgacaaaata gtatcgccgc
     4321 tgtcgatcag atcgcgcacg gtgtggttga tcggtaagcc cgcaccgacg gtggcattgt
     4381 acagccagtg acgcccggtt ttttcgaagg cgtcgtggat ctggcgatat ttattgctgt
     4441 cgctggctcc cgccagtttg ttggcgctga tgacgtggaa accgtggctg gcgaaatcaa
     4501 gatactgatc agcaagctgc tggctggcgg taacgtccag caccactaaa tcatcatacg
     4561 gatgggcgcg catccacagg aacaacgact cttcatcctg ctcaaccgct tcatcgttga
     4621 agaaggctaa cgcgcggctg gcgtccagcc cgtcatagct caacagactg cggcggctgt
     4681 ccaccacgcc tgccagcaca aattcaaagt cggtacgtgc cgaaagcgtg ctctgctcac
     4741 gggcgaacag ttccagccaa cgggaaccga tattgccctt accgaacaat accaggccga
     4801 tgcgtttttc tgcgcggaag acggactgat gcagcccctg aatcaggctt tcggtcgggc
     4861 cggtgcgcag tactgccacc aggctgatgc cgtcatcgga ctgccaggta aattcgaccg
     4921 gctggccttt cagttgctgc cagaagcggt ggcaatgcag cgggttacgg gtgacgcctg
     4981 caccgaccat cgccaccagc gccagcccct gacgcaggcg cagttcgcca ggtaaccccg
     5041 cttcgtcgag gattttcagc gcactgtcgg ccacttctga ggtgtagcaa aattgcagca
     5101 actggcgatc gttatgtacg ccaaccgcca gcgggcgtac ctgcgcgcgt ttcaggattt
     5161 ggtcgatctc tttatgcgcc agtttgaaat cctgactggc gggcacctga aactcaatca
     5221 aacagacatc atcgtggctg gtgacaatac gcgcaccagt accggaggcc agcacgcgtt
     5281 caatgcgcgt ggaaccttga tccggcgtgt agctacagcg cagttgcagg tcgatttcgc
     5341 tgccagaaac cggctgtaaa gtacgggcgt gaagaacggg agccgccaga cgagccagtt
     5401 cgctggcttc atccagacgc agcaacggca gcagacaggc atctttcact ttacgcgggt
     5461 cggcactgta taccccggcg acgtcgctcc agatggttac acgagaaaca cccgccagcg
     5521 caccgatttg tgtcgcggaa tagtcggaac cgttacgccc cagcagcacc gtttcaccgg
     5581 cgttgttacg gctgataaac ccggtcacca ccagacgttt gcccggatgt tgcaccagta
     5641 gctgttgcag caacgggtaa gaaagccctt catcaacctg cggttgtgcg gcgcgttcag
     5701 cgcgtaaaaa ctcgcgggca tcaagccagg ccgctggcag cccttgttga ttaagtaccg
     5761 cagacatcag gcgtgccgac catacttccc cgtggcccac cacttccgca tacactgcgt
     5821 cgttaatacc gctgtcgagc agcgccgcca ggcgttccag atcgctgaca aaagcgctaa
     5881 tgaggctatc ggcttcttca gcgggtagca gaccgctaat cagatcgcac tgatagcgac
     5941 gcagcgtttg ttgaacctga tgcgcagaga gacgatcggt ctggcttagt ttcaaccagt
     6001 taatcaactg gttagtggtg ctaccggcgg cggaaaccac catcatatcg tcaggctgag
     6061 agtactccgc cataatgccc gcgacacgca aataacactt cacatcagcc agactactgc
     6121 caccaaattt atgcagctga cgacctttcg cccctgcctg cgcaatcaca ctcattttta
     6181 ccccttgttt gcagcccgga agccattttc caggtcggca attaaatctt cgccatcttc
     6241 aataccggtg gagatacgca gcagcgtctc ggagatcccg gcggcagcac gcgcttctgg
     6301 tgccatgcct gcatgtgtca tggttgcggc gtgagagatt aaactttcca ctccccctaa
     6361 tgattccgcc agcgtaaaca acgacagccc gctcaggaaa cgacgcagcg tctgctcatc
     6421 gccatccagt tcaaaactca acattgcgcc aaagcctttt tgctgacgcg cggcaatttc
     6481 atgcccctga ttttccggca gcgacgggtg atacagtttt ttcaccaacg gctgggtttg
     6541 cagatatttc acaatcgcct gcgcgttgcg ctgcgccagc tccatacgcg gcaccagcgt
     6601 tcgcaacccg cgtagcagca gatagctgtc aaacgcgccg cccgtcacgc caatattgtt
     6661 tgcccaccag gcaagttcag tgacaacgtc cgggtcttta gcaatcacca cgccagccac
     6721 cacgtcagag tgaccgttca gatatttcgt gcatgaatgc aacaccagat cggcacctaa
     6781 tgccagcgga ttttgtaatg ccgggcttaa gaaggtgtta tccaccacgc tcaccgcccc
     6841 gacttccctt gccagatggc agattttcgc aatatccacg acgcgtaaca atggattact
     6901 tgggctttct accagtacca gtttgggttt ttctgccagc gctgcccgta atgcctgttc
     6961 atcgccttga tcaacaaaca acacgcgata gcaaccgcgt ttcgccagac tgtcgaacag
     7021 gcgatagcta ccgccgtagc agtcgtgcgg cgcaaccagc agatcgccag gtttcaaaaa
     7081 gacggtcgtt accaggtgaa tcgcggacat gccggtatta gtaagtactg cgccagcacc
     7141 gccttccagt tctgccagcg cacgctgaac cacatcgcgc gttgggttgc cgcgacgcga
     7201 gtaatcatgc gcgcgcggtt cattaaatcc ggtaaagtta taggtgctgg aaagatggat
     7261 gggagggaca acgcaaccat actgttcgtc gtcatttaac ccgctacgca ctgcgatggt
     7321 ggcctgttta cgcgtcatgt gatgaagttt cctgggcttt gtcggtgaaa tgtcaggcac
     7381 cagagtaaac attgtgttaa tggacgtcaa tacatctgga catctaaact tctttgcgta
     7441 tagattgagc aaatcccaaa tagccgttaa aattatatgc attatcacgc cgacaggtgc
     7501 attacacgat gtcacggtaa cgcctgtacg gtaaactatg cgggtttacg gtcagtaccc
     7561 acataaattg tgtggtctgg tctcaattta ttgacgaaga ggattaagta tctcatggct
     7621 gaatggagcg gcgaatatat cagcccatac gctgagcacg gcaagaagag tgaacaagtc
     7681 aaaaagatta cggtttccat tcctcttaag gtgttaaaaa tcctcaccga tgaacgcacg
     7741 cgtcgtcagg tgaacaacct gcgtcacgct accaacagcg agctgctgtg cgaagcgttt
     7801 ctgcatgcct ttaccgggca acctttgccg gatgatgccg atctgcgtaa agagcgcagc
     7861 gacgaaatcc cggaagcggc aaaagagatc atgcgtgaga tggggattaa cccggagacg
     7921 tgggaatact aaagcaaaaa agagcggcgc ggagtggaat cgcctgatgc gctacgctta
     7981 tcaggcctac gtcatattgc aatttattga atttgcacga acttgtaggc cggataaggc
     8041 gttcacgccg catccggcat aaacaacgag cacgttgtct gcgacccacc gctttttata
     8101 catggacgtt taactatgaa aaacaggctg ctgatcctca gcctgctggt ttctgtacct
     8161 gcctttgcct ggcagccaca aaccggcgac atcatctttc agatctctcg ctcatcgcaa
     8221 agtaaagcga tccaactggc gacccattcc gattatagcc acaccggtat gctggtgata
     8281 cgcaacaaaa agccctacgt ttttgaagcg gtcggcccgg tgaaataccc cccgctcaag
     8341 cagtggatcg cccatggtga aaagggcaaa tacgttgttc gccgcgttga aggcggactg
     8401 agtgttgaac aacagcaaaa actggcgcaa acggcaaaac gttatctcgg taaaccgtat
     8461 gatttcagct tctcatggag cgacgatcgc cagtactgtt cggaagtggt gtggaaggtt
     8521 taccagaacg cgctgggaat gcgcgtgggc gagcagcaga agttgaaaga gttcgatctc
     8581 agcaacccgc tggtccaggc taagctcaaa gaacgctacg gcaaaaatat tccgcttgag
     8641 gaaacggtag tttcaccaca agccgttttc gacgcgccac aactcaccac agtcgccaaa
     8701 gagtggccgc tgttttcgtg gtaatggatg atgaaaaaaa taattcccgc cgtagtctgt
     8761 ttgttgcttt gtagttgcac gacctttccg caagctgtcg ctccggtaaa cggtgatgcc
     8821 tccaattagt tgaattaagg atataatact cgcttttaag gttgtttcat ggtcacagtt
     8881 aaccttcatt gcccccgttg ccagtccgtt caggtttacc gtcacgggca gaacccaaaa
     8941 agacatgagc gattccgcgg ccgtgactgt caccgggtat ttcagctcag ttactgttac
     9001 gagccccgta aaccgggagt taaagaccaa atcgctgaaa tgactttcaa cggtgccggg
     9061 gttcgcgata ctgcccgtac gctgaaaatc ggcattaata cagtcatccg aactttaaaa
     9121 aactctcgcc acgaagaata acgtcatccc cggtcgctca tgctgatgtg gcactcatct
     9181 gcaaacttag tcagcaatgg agctttgtcg gcagtaaagc gcgacagcac tggctctggt
     9241 acgtgtacaa cacgaaaact ggtggtgttc tggcctacac ttttggcccc cggactgacg
     9301 aaacctgtcg tgaattgcgg gcgctgctta cccttttacc atcggcatgt tgaccactga
     9361 cgaatggggt agttacgcta gagagttacc gaaggagaag catctgactg gtaagatttt
     9421 tactcagcac attgagcgta acaatctgac cctgagaacc cgaatcaaac gtctggcccg
     9481 cagaaccatc tgtttctcgc gttctgtaga acttcatgaa aaagtcatcg gtgcttttat
     9541 cgaaaatata tgttctacta attggagtca tcacccttct gataagcttt ccttggatga
     9601 ttaccagact gaatacacag ccaaagattt ttacctcatt ctgctctttt ttctcaaatg
     9661 acatatatta tattttacat catattcata ggcatatgac attcccccgc cccccattat
     9721 gtcattgtgg gttgatgttg ggctttcata aaaaaaacat ctttttttga actagcatta
     9781 attatattta ctttatatat taaaatattt tctccatctg gaaatatttt agccacacaa
     9841 tataaagcag ctgaatccga attacttaaa aatatattat catcacccat aactccagcc
     9901 attaaatgac tggagactaa agcaaggaaa taacaacatt gtattgcctt cattttaatt
     9961 actcccttgc ctgataattg taactccatt ccctttagct ttgggattat attttagata
    10021 ggtgttattt ggtgttccca agtaactact gtattctttt tttcccattc cattcatata
    10081 agaattcgtt aaatctttgc ttgaaaaatt tagactatca taaacatcat tttcttttgt
    10141 tactttattt cctttatcgt cagagtaaaa accgtgagtg tgataatcgc ctactctctc
    10201 tgaaccttca gggcatttta tatttcgcaa gtctacacta tcattatcat ttgaacttat
    10261 cggattcatg gggaaatatt caccttgctt cttacatatt agtccaccat attctttatt
    10321 ctcgttaatt gattctccat tacacattgc caatgcaaat attccaatat catcaatatt
    10381 ttttgattta aattcatata atcctaatgg atccatgcta ttcaccgggc tcagcggata
    10441 tgtatacagg ttccatcccc ccttcagccc aatcggatcc tgagtgatat accgcccctg
    10501 cagcgggtca taatagcggt ggcggttata atacaggccg gactcctcat catactgctg
    10561 ccccggcaaa cgaataagct gctgcagctg atgcgggttc tcttcattca gcaggttgcc
    10621 ccattcatca tattcccggc gtatttccag cccgtcaggc ccggtggctg acgttaactg
    10681 gctgtggtgg ttatagtaaa acgccgatgc cctgccatcc ggcgtggtga tgcgcgtgat
    10741 gagacccgtc accacatccg ggctgtattc tgttgtccgg cctgcggcat ccgtctgtgt
    10801 cctgagcctg cccacggcgt caaactgact ctgcgtgacg ctgccgtccg cgtgttcctt
    10861 tttcaccacc cgcttcagcc cggcttc
//
LOCUS       DAZPAC010000138        10718 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZPAC010000138 DAZPAC010000000
VERSION     DAZPAC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.138"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..1093
                     /gene="dacC"
                     /locus_tag="ACHUPM_002968"
                     /pseudo
     CDS             1..1093
                     /gene="dacC"
                     /locus_tag="ACHUPM_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1140..1898)
                     /gene="deoR"
                     /locus_tag="ACHUPM_002969"
     CDS             complement(1140..1898)
                     /gene="deoR"
                     /locus_tag="ACHUPM_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR0870684.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1956..2552)
                     /gene="ybjG"
                     /locus_tag="ACHUPM_002970"
     CDS             complement(1956..2552)
                     /gene="ybjG"
                     /locus_tag="ACHUPM_002970"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0870685.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2837..4064
                     /gene="mdfA"
                     /locus_tag="ACHUPM_002971"
                     /pseudo
     CDS             2837..4064
                     /gene="mdfA"
                     /locus_tag="ACHUPM_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4105..4389)
                     /gene="ybjH"
                     /locus_tag="ACHUPM_002972"
     CDS             complement(4105..4389)
                     /gene="ybjH"
                     /locus_tag="ACHUPM_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR0870686.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4475..5290)
                     /gene="ybjI"
                     /locus_tag="ACHUPM_002973"
     CDS             complement(4475..5290)
                     /gene="ybjI"
                     /locus_tag="ACHUPM_002973"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR0870687.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5290..6498)
                     /locus_tag="ACHUPM_002974"
     CDS             complement(5290..6498)
                     /locus_tag="ACHUPM_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0870688.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6582..7118
                     /gene="rcdA"
                     /locus_tag="ACHUPM_002975"
     CDS             6582..7118
                     /gene="rcdA"
                     /locus_tag="ACHUPM_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR0870689.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7293..8978)
                     /locus_tag="ACHUPM_002976"
     CDS             complement(7293..8978)
                     /locus_tag="ACHUPM_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR0870690.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9248..9625
                     /locus_tag="ACHUPM_002977"
     CDS             9248..9625
                     /locus_tag="ACHUPM_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR0870691.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9655..9912)
                     /gene="grxA"
                     /locus_tag="ACHUPM_002978"
     CDS             complement(9655..9912)
                     /gene="grxA"
                     /locus_tag="ACHUPM_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR0870692.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10072..10359
                     /locus_tag="ACHUPM_002979"
     CDS             10072..10359
                     /locus_tag="ACHUPM_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR0870693.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10343..>10718
                     /gene="nfsA"
                     /locus_tag="ACHUPM_002980"
     CDS             10343..>10718
                     /gene="nfsA"
                     /locus_tag="ACHUPM_002980"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR0870694.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLG"
BASE COUNT         2765 a         2650 c         2715 g         2588 t
ORIGIN      
        1 gatgcgcgtg catggatttt aatggattac gccagcggta aagtgctggc agaaggcaac
       61 gcggatgaga aactggatcc cgcgagcctg actaaaatca tgaccagcta tgtggttggg
      121 caggcgctta aggccgataa gattaaactc accgatatgg tgacggtcgg taaagatgcc
      181 tgggcgacgg gaaatccggc actgcgtggt tcatcggtaa tgttcctcaa accgggcgat
      241 caggtttcgg tggcagactt gaacaaaggt gtgattatcc agtccggtaa tgacgcctgt
      301 attgcgctgg ccgattacgt tgccgggagc caggagtcat ttattggttt gatgaatggt
      361 tatgccaaaa aaactgggtc tgaccaacac taccttccag acggtgcacg gtctggatgc
      421 gccggggcag ttcagtaccg cgcgcgatat ggcattgctg ggtaaagcat tgatccacga
      481 tgtgccggaa gagtacgcca ttcataaaga gaaagagttc accttcaaca aaattcgtca
      541 gcctaaccgt aaccgtctgc tgtggagcag caatctgaat gttgatggca tgaagacagg
      601 aaccacggca ggtgcgggat ataatctggt tgcttcggct acccagggtg atatgcgttt
      661 aatctccgta gtgctggggg cgaaaactga ccgtatccgt tttaatgagt ctgagaaatt
      721 attgacctgg ggtttccgct tctttgaaac tgtgacgcca attaaacctg atgccacctt
      781 tgtgactcag cgcgtctggt ttggtgataa gagcgaagtg aatctcgggg caggcgaagc
      841 gggctccgtg accataccgc gtgggcagct gaaaaacctg aaggcgagct acacgttaac
      901 ggaaccgcag cttaccgcac cgctgaaaaa aggtcaggtt gtcgggacca ttgatttcca
      961 gcttaacggt aaatccattg agcagcgtcc gctgatcgtg atggaaaatg tggaagaggg
     1021 cggattcttt ggtcggatgt gggatttcgt gatgatgaaa ttccatcagt ggttcggcag
     1081 ctggttctct taatcttctg ataaccggat ggcgcgaaac gtcatccggt tatacgtcat
     1141 taatacatca acttaatacg gtgcgtctgc gcgtacttca catactcatc ttccgggcaa
     1201 caatcactca ccacaatatc aaagcgtttc aggtcaccca tgcgcgccgg acgcaccttg
     1261 ccaaatttac tgtggtcgac aaccagcaca tgcttttgcg ccatcgacat ggcccagtgt
     1321 tttaccggca actcttcaag attaaaacag gtagcgcctt tactgacatg cacgcccgcc
     1381 gcggaataaa aagcgatatc cgggcaaaaa ttattcaacg tttgctgaaa atcgatgggg
     1441 ttgaaaatgg cgttgctggc gtgaaattca ccaccgcaca gaaatgcgcg gcaatggggt
     1501 ttctctttca gcgccagaaa ggtatttagc gaataacaaa cggcggtaaa agggatttca
     1561 ttatcaatcg cttcaataat ccacggcgtg gtggtgccac agtcaaaaaa gagggtctga
     1621 tcgggttcta ccagtgtcgc agccagtttt gccgcccggc gtttttcttc caccaggcgg
     1681 gatttttgat cgcttaacag gtaatggctg gcactgcgcg gttccagaac aatatagccg
     1741 ccgagcaaaa cgacgggcgc actgtggttg ttcagatcgc gacgaatcgt catctccgaa
     1801 accccaagca gggcggcggc gtctttaaga tgtaacttat cgctgcgttt taattcttgc
     1861 agcagctgcc cgatacgctc ttcgcgacgt gtttccataa tccctctgaa tagttattga
     1921 agcgagccgc tcaatactac actttttagc agagatcagt cacgcaccca gcctttgcgg
     1981 atcggtaatg caaaacagac gcgataccac gattgcagac gttgatagag tttatgcccc
     2041 atcgcttgcc agataatctg ggcactaagg cagccaatca tacctgccag caatccaccg
     2101 agcatatcca gcggccagtg gacgccaaga taaacgcgcg accaggcaat gacgacggcc
     2161 agaaccatta aaagtgagcc ggaccacagg cgatgccagc ataaaaatgc cagtgcaaag
     2221 gtgaaaatca ccgtaccgtg atcgctcggg aatgagtcat ccgccgcatg atgcaggaag
     2281 ttatagccga tattttcgac aaagggtcgg tcgtgcggaa aaagatgtcc catcgtccag
     2341 gacacaaaca ggctgaccgc cagcgcgata gcgattttta tcaccagttg ccgttgtgct
     2401 gtaagccccc acaaccaaag taccgcggcc agcaacggca ccacggtaat caaatcttta
     2461 gcaataaaaa tcgccaacga gatcatccac ggagccgagt ctggcgtcgc gttaataaga
     2521 gagaatagag agagattcaa attttccagc ataacttccc gacgcaaagt gattaaaagg
     2581 gagccaatac aggcaagtcg ttgagaataa agtgcaggtt aaactgggta aagcggcatc
     2641 gtcttatttc cctcaagcgg cctgtttacg gtgggtgatt gtaacgggca taggttaaat
     2701 aaaacttaaa gaaagcgtag ctatactcgc aataatgtaa gaatgtgctt aaccgtggtt
     2761 tcagctacaa aattcgcttt ctcgttagct gcgcttttat taaactctgc gcgattatta
     2821 ttggcgaaga aattgcatgc aaaataaatt agctttcggt gccaggcttg gacgtcaggc
     2881 gttacttttc cctctctgtc tggtgcttta cgaattttca acctatatcg gcaacgatat
     2941 gatccaaccc ggtatgttgg ccgtggtgga acaatatcag gcgggcattg attgggttcc
     3001 tacttcgatg accgcgtatc tggcgggcgg gatgttttta caatggctcc tggggccgct
     3061 gtcggatcgt attggtcgcc gtccggtgat gctggcgggt gtggtgtggt ttatcgtcac
     3121 ctgtctggca atattactgg cgcaaaacat tgaacaattc accctgttgc gcttcttgca
     3181 gggcataagc ctctgtttca ttggcgctgt gggatacgcc gcaattcagg aatccttcga
     3241 agaggcggtt tgtatcaaga tcaccgcgct gatggcgaac gtggcgctga ttgctccgct
     3301 acttggtccg ctggtgggcg cggcgtggat ccatgtgctg ccctgggagg ggatgtttgt
     3361 tttgtttgcc gcattggcag cgatctcctt tttcggtctg caacgagcta tgcctgaaac
     3421 cgccacgcgt ataggcgaga aactgtcact gaaagaactc ggtcgtgact ataagctggt
     3481 gctgaagaac ggccgctttg tggcgggggc gctggcgctg ggattcgtta gcctgccatt
     3541 gctggcgtgg atcgcccagt cgccgattat catcattacc ggggagcagt tgagcagcta
     3601 tgaatatggc ttgctgcaag tgcctatttt cggggcgtta attgcgggta acttgctgtt
     3661 agcgcgtctg acctcgcgcc gcaccgtacg tgataattat gggcggctgg ccgattatga
     3721 ttggtctgtt ggtcgctgct gcggcaacgg ttatctcatc gcacgcgtat ttatggatga
     3781 ctgccgggtt aagtatttat gctttcggta ttggtctggc gaatgcggga ctggtgcgat
     3841 taaccctgtt tgccagcgat atgagtaaag gtacggtttc tgcggcgatg ggaatgctgc
     3901 aaatgctgat ctttaccgtt ggtattgaaa tcagcaaaca tgcctggctg aacgggggca
     3961 acggacagtt taatctcttc aaccttgtca acggaatttt gtggctgtcg ctgatggtta
     4021 tctttttaaa agataaacag atgggaaatt ctcacgaagg gtaaaaaaat gcctgactgc
     4081 tttgtgcgat caggcattct cgaattaatg gtgatggtcg tcaatctggt gttcgataac
     4141 catcccttca cctacgctgg caagatggcg agcataagga tgcgggcggt aagccggagc
     4201 tggcgcagga gccacataga cggtttgcgg cacagtcgcg acgctgaccg cttgtggaac
     4261 gctgactgaa tcaggaacta caaccacttt gtatccactc ggcacatcga cggtgacact
     4321 ttgcgccatc gccacaccag taaagcccat taaaagcgcg cccaacagta gacaattttt
     4381 cataatcatt tcgcctgaaa taatgcagcg ggttaatttc cgcgaattat gcagccttca
     4441 ttcaggtatt gtgttgccgg gcgtgctgcc gtttttattg gtcaaatggc gcttcgtgtt
     4501 taagcacttt atcgatcaca tccagtacgc cttcacggtt attggagcct gcccggtatt
     4561 ttgccgctgc gacgaccgcg ctgccggcat tttccattgc aaaactaaag cctgcctgac
     4621 gcagcatctc aatatcgtta ccgccatcgc caaagaccac cacttcgctg tcgtctattc
     4681 cccataattt ctgcagttgg cgaaggccat tggctttatg tacgccgggg ataatcagat
     4741 cgatgctgcc gttgccggtg tggactggca ccataatatc gccgatggcc tcatgtaatg
     4801 ctttttgtac ttgtggaatc agttcatcgg aaagattcag gccaaactta aagaaaatat
     4861 cttctaagtt gtcaaagtta tcgacgtatt ccagacggtg ataatacatt tctgccaccg
     4921 ttttcatggc atcgtcatat tttttgagcg tataggcgct attttttccg caggcaataa
     4981 tttccacttc cgggcgcgtc agcaaatgtt ccacgacagt agtaaaggca tccttcgaca
     5041 gctcgccatt aaaaacatct ttgccttcac tcactaccca gccgccgttt tcagccacaa
     5101 aggcaatttc attagcaatt tcagggaaga aagagagcaa ctgataatat tgattcccgc
     5161 tggcgaccac aaagcgaatg ccttgtgctt tcatttgctg atactgagcc ataaaccgct
     5221 cacggttata ggttttttga tcgcttaaga aagtaccatc catgtctacc gcaattaatt
     5281 taatgctcat caactattct ccatcgccgt ccgcgttttg gtatcgggtt tggcgacggc
     5341 tttcgcgaca atagccgcga gaataaccag cgccagtaca accagcattg cactacgtaa
     5401 tccataatgt tcgccgagat agcccagcag cggcggcccg acgaggaaag ccagataacc
     5461 ggtcgttgct accacactga cgcgggttgg tgcatcgggg ccggtatcgc tggcggcaga
     5521 aatggtcagc gggaagccca gcgaggcacc cagtccccag agtacaacag acaccccagc
     5581 gacccaggcg ctatcgacaa aaataatcag cccaataccc aacgccccca ttagtgcact
     5641 ggcccgaacc acggcaacgc gactgtaacg gtcgatgaac caaccgccag tgaagcgtcc
     5701 aacggtcatc cctagggtaa aaccggcata aatcagcgag ccggaagtag ggctaaaacc
     5761 gtgaccgtca accattaata agggtaacca gtcgttggca gaaccttcgg caaaggccat
     5821 cgccagcact acaacaccaa tcaacagcaa ctggatatcg cgataaaaag gaatgccttt
     5881 ttcgccatgc tgggtgccat cggcagcatt tttgcccgta ccgtcaggga ttgcctgaat
     5941 ggcgatataa ataggcgcga tacctaccag cgccgccaat aaaatgtgca ccgttgccgg
     6001 aacgccaaag gccgtcagtg ccatcccgac accagcgcct gccagcgtgc ctaggctata
     6061 aaaaccgtgc atcatcggca aaaccgtttt attcatttct cgctcaacgg cggcaccttc
     6121 aacgtttatc gccacttccg cagaaccaaa acttgcccca aagacgccga gaccaacggc
     6181 aaagagcagg ggcgatgtca gccagagtgc cagacttaat atcatcatcc cgatcaatgc
     6241 gcaggacatc gtgaccagga tgacattacg tgtcccaaag cgtttcacta accacgccga
     6301 gcagagaata ccgctcatcg aaccgatcga cagaccaaag agaacaccgc ccatttcagc
     6361 gatcgagaca gagagaatat cgcggatagc aggcgtacgg gttgcccagg acgccattaa
     6421 caggcctggc aaaaagaaga acataaacag cgcccaggtt cggcgtttca atgcattacg
     6481 tgaagaattt acggtcatag atcacgtcaa aataagaaga gggaagacaa cattagcaag
     6541 gttgtgtaca tttgtacaca attgcagaca gaggaaatga catgcgtcgc gctaacgatc
     6601 cgcaacggcg agaaaaaatt atccaggcca cgctggaggc ggtgaaactt tacggaatac
     6661 atgctgttac gcaccgcaaa attgctaccc ttgccggggt accgttggga tcgatgacct
     6721 actatttttc aggaattgat gagttgttgc tggaggcgtt cagcagtttt actgagatta
     6781 tgtcccggca atatcaggca ttttttagcg atgttagtga tgctcaaggc gcatgccagg
     6841 ctatcaccga tatgatctac agctcacagg ttgcaacgcc ggataatatg gagctgatgt
     6901 accagctcta cgcgctggct agccgaaaac cgctattaaa aacggtaatg caaaactgga
     6961 tgcagcgcag tcagcaaacg ctcgaacaat ggtttgaacc cggaaccgcc cgcgcgcttg
     7021 atgcgtttat tgaggggatg acgctgcatt ttgtcaccga ccgtaagccg ctatcgcgcg
     7081 aggagatttt gaggatggtt gagagggttg cagggtagta gataaatttt agataacaaa
     7141 aaacccatca accttgaacc gaaatggcgg ggttgatggg ctccacaaaa tggggacatc
     7201 aaagaaaagc agtggcacta attaagactg atgccctgcg gaaaagttct gcggttgtgc
     7261 aaaaaaattt cattttcagg gcaacttcag ttttatccta atcctggcca taccatgacg
     7321 atgattgtcc ctgccagcgt cagcaggacg ttggcgattg cgtaggtgcc cgcatagccc
     7381 agcgcaggga tgttactgcg agctgtatca ctgatgatct ccattgccgg cgcgcaggtg
     7441 cgtgcgccca tcattgcgcc gaacaacagt gcgcggttca ttcgcaatac ataagcaccg
     7501 aacaagaaac agataaccac gggcacaaga ctgacaatta atccggcaat caacatctga
     7561 ccgccaatcg cgcccaggcc gttattaata ccgctaccgg cgctcagacc aacgcctgcc
     7621 ataaacacca tcaagccgaa ctctttcacc atgcttaatg caccttgcgg aatgtaaccg
     7681 aaggtcgggt ggttagcacg cataaagccc agcataattc cggcgaataa caacccggca
     7741 gcgttcccca tgccgaaact gaatgtgctg aactggaagg tgatcatccc gatcatcagc
     7801 ccaataacaa agaaggcgca aaatgccagc aggtcagtga cctggctgtg aatcgagata
     7861 aagccgatgc gatcggcgat ggtttttacg cgacgggcat caccgctgac ttgtaaaacg
     7921 tcacctttgt taagcacgac gttgtcatct atcggcatct caatctggct acgaatgacg
     7981 cggttaagga agcaaccgtg atcggtcaac ttcagttgtg cgagacgttt acctacagcg
     8041 ttatggtttt taacgaccac ttcttcagtg acgatacgca tgtcgagaag gtcacgatcg
     8101 aaaacttctt taccgttacg gaagctggga tcgagtcggg catgggcgtc gggatagcct
     8161 accaacgcta tttcatcgcc catttgtagc acggcatcac cgtctggatt tgccagaatc
     8221 ccgttacgtc gaatacgttc aatgtagcag ccggtttgtc gataaatacc cagttcacgc
     8281 agatttttgc cgtcggtcca ggccaccagc tccgggccga cgcgataggc gcggatcacc
     8341 ggtaaataaa ccttacggtt ggcatcagtg tccaggccac gttcgcgggc gatttgctgg
     8401 gcgctggtct gtaagtcctg atgctgcaat ttcggcaagt aacgcgcacc aacaatcaaa
     8461 ctcaccagac cgattaaata ggttaaggca tacccgaggc tcagattatc cagtgccagt
     8521 gagagctgcc tgctttccat gccggaatga cgcagtgtat cgccagcacc gaccagaacc
     8581 ggtgtcgacg tcatagagcc tgctaacata ccggccgtca ggccaatatc ccagccaaac
     8641 agcttaccta accctaaggc gatcaccagc gcactgccaa ccatcaccag tgctaacatt
     8701 aggtaatttt tcccatcgcg aaaaaaaatg gaaaaaaagt tcggtccggc ttcgaccccg
     8761 acgcagaaaa taaacagcat aaagccaaga ttaagcgcat cggtgttaat gctgaaatgt
     8821 tgttggccta ataacagcga tacgactaaa acgccaatgg aattacccag ttggatcgaa
     8881 ccaagtcgta actttccgag acatagccca agcgtgagga ccacaaataa taacagaatg
     8941 taattcccat ttaacaattc ggcgacgttt atattcacgg aggctaactt cttgtttact
     9001 agtaagctgt tgaaagaaat ggtaatttac gataatgttt tttaccagaa ttcagggcgc
     9061 agattcattc agcgcaccta aacgatagta aagtaacaat atattttact agtgtaatca
     9121 cattaggtat caacggctat atgaattgcg ttggcctata ttagcatgga atgcgaagcg
     9181 gctttatctt actgaacgcc acactggcga aaaatgtgtt cgatagacgc agtgtcagga
     9241 ggaacgagtg aaacataaac aacgttgggc gggggcaatc tgctgttttg tcctcttcat
     9301 tgtggtgtgc ctttttctgg cgacgcacat gaaaggcgct tttcgggctg ccgggcatcc
     9361 tgaaatcggc ttgctgtttt tcattcttcc tggagcagtt gccagtttct tttcacagcg
     9421 tagagaagtc ctgaaacctc tatttggcgc aatgctggcg gcaccctgtt caatgctcat
     9481 tatgcggctg tttttttcac cgacgcgctc attctggcaa gagctggcat ggttactaag
     9541 cgcggtgttc tggtgtgcgc tgggggcact gtgtttctta tttatcagta gtttgtttaa
     9601 accacagcac agaaaaaatc agtaaagccc tcaacgcgag ggcttgtcag acgatcaggc
     9661 gtccagattt tctttcaccc atgcagcaaa atcggtatag ccgccgatat gttgctgatc
     9721 gacaaaaatc tgcggcacgg tttctacggg tttacctgcc ttttgttgta gatcttcttt
     9781 agtgatccct tccgcacgaa tatctacata ctgatactga aaatcatcgc gttcattgct
     9841 caatttctca gccagatctt ttgcacgcac acagtaaggg caacccgaac gaccaaaaat
     9901 aacggtttgc attatttctc tcctcataga tttatgcctg taatgatcac gctaaaatgt
     9961 attcgctgaa agtaggttta acctgttgca ttaattgcta aaagctataa ctgttaaaca
    10021 caatacagtg aaaagtttta gactgaaggc tcactttgca gagggaagcg tatgcgcgcg
    10081 atcggtaaat tgcctaaagg cgtgttgata ctggaattta tcggaatgat gctactggcg
    10141 gtggcgctgc tgtcggtaag cgactccctg tcgctgcctg agccattttc tcggccagaa
    10201 gtgcagattc tgatgatttt tctcggtgtt ttgctcatgc ttcccgctgc ggtggtggtt
    10261 attcttcagg tggcaaaacg tcttgcccca cagctgatga accgtccacc gcaatattca
    10321 cgttcagaaa gagaaaaaga taatgacgcc aaccattgaa cttatttgtg gccatcgctc
    10381 cattcgccat ttcactgatg aacccatttc cgaagcgcag tgtgaggcga ttattaacag
    10441 cgcccgtgcg acgtccagtt ccagtttttt gcagtgtagt agcattattc gcattaccga
    10501 caaagcatta cgtgaagaac tggtgacgct gaccggcggg caaaaacacg tagcgcaagc
    10561 ggcggagttc tgggtgttct gtgccgactt taaccgccat ttacagatct gtccggatgc
    10621 tcagctcggc ctggcggaac aactgttgct cggtgtcgtt gatacggcaa tgatggcgca
    10681 gaatgcatta accgcagcgg aatcgctggg attgggcg
//
LOCUS       DAZPAC010000139        10686 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZPAC010000139 DAZPAC010000000
VERSION     DAZPAC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.139"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /locus_tag="ACHUPM_002981"
     CDS             complement(<1..784)
                     /locus_tag="ACHUPM_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR0870695.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="ACHUPM_002982"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="ACHUPM_002982"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR0870696.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="ACHUPM_002983"
     CDS             1860..2747
                     /locus_tag="ACHUPM_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR0870697.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="ACHUPM_002984"
     CDS             2780..3790
                     /locus_tag="ACHUPM_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFR0870698.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="ACHUPM_002985"
     CDS             3860..5293
                     /locus_tag="ACHUPM_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0870699.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="ACHUPM_002986"
     CDS             5392..6741
                     /locus_tag="ACHUPM_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0870700.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="ACHUPM_002987"
     CDS             6731..7618
                     /locus_tag="ACHUPM_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR0870701.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="ACHUPM_002988"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="ACHUPM_002988"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR0870702.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="ACHUPM_002989"
     CDS             8352..9224
                     /locus_tag="ACHUPM_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR0870703.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="ACHUPM_002990"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="ACHUPM_002990"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR0870704.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="ACHUPM_002991"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="ACHUPM_002991"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR0870705.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2461 a         2532 c         2855 g         2838 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagt attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccggg taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tttcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggt
//
LOCUS       DAZPAC010000140        10662 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZPAC010000140 DAZPAC010000000
VERSION     DAZPAC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.140"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="ACHUPM_002992"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="ACHUPM_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR0870706.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="ACHUPM_002993"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="ACHUPM_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR0870707.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="ACHUPM_002994"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="ACHUPM_002994"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR0870708.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="ACHUPM_002995"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="ACHUPM_002995"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR0870709.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="ACHUPM_002996"
     CDS             complement(5254..6294)
                     /locus_tag="ACHUPM_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR0870710.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="ACHUPM_002997"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="ACHUPM_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR0870711.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="ACHUPM_002998"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="ACHUPM_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR0870712.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="ACHUPM_002999"
     CDS             7229..7669
                     /locus_tag="ACHUPM_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR0870713.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="ACHUPM_003000"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="ACHUPM_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR0870714.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="ACHUPM_003001"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="ACHUPM_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR0870715.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..>10662
                     /gene="rsxC"
                     /locus_tag="ACHUPM_003002"
     CDS             8898..>10662
                     /gene="rsxC"
                     /locus_tag="ACHUPM_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR0870716.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRK"
BASE COUNT         2454 a         2756 c         2724 g         2728 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat taaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcctctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc gcgccaaagc gcgcaagctg gaacagcaac
    10621 aggctaatgc ggaaccagaa gaacaggtcg atccgcgcaa ag
//
LOCUS       DAZPAC010000141        10473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZPAC010000141 DAZPAC010000000
VERSION     DAZPAC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10473
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.141"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            145..720
                     /gene="ssuE"
                     /locus_tag="ACHUPM_003003"
     CDS             145..720
                     /gene="ssuE"
                     /locus_tag="ACHUPM_003003"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR0870717.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            713..1672
                     /gene="ssuA"
                     /locus_tag="ACHUPM_003004"
     CDS             713..1672
                     /gene="ssuA"
                     /locus_tag="ACHUPM_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR0870718.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            1669..2816
                     /gene="ssuD"
                     /locus_tag="ACHUPM_003005"
                     /pseudo
     CDS             1669..2816
                     /gene="ssuD"
                     /locus_tag="ACHUPM_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            2828..3619
                     /gene="ssuC"
                     /locus_tag="ACHUPM_003006"
     CDS             2828..3619
                     /gene="ssuC"
                     /locus_tag="ACHUPM_003006"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR0870719.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3616..4383
                     /gene="ssuB"
                     /locus_tag="ACHUPM_003007"
     CDS             3616..4383
                     /gene="ssuB"
                     /locus_tag="ACHUPM_003007"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0870720.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4590..7202)
                     /gene="pepN"
                     /locus_tag="ACHUPM_003008"
     CDS             complement(4590..7202)
                     /gene="pepN"
                     /locus_tag="ACHUPM_003008"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR0870721.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7468..8670
                     /gene="pncB"
                     /locus_tag="ACHUPM_003009"
     CDS             7468..8670
                     /gene="pncB"
                     /locus_tag="ACHUPM_003009"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR0870722.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8843..10243
                     /gene="asnS"
                     /locus_tag="ACHUPM_003010"
     CDS             8843..10243
                     /gene="asnS"
                     /locus_tag="ACHUPM_003010"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR0870723.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2357 a         2696 c         2824 g         2596 t
ORIGIN      
        1 gtgatatata aagccctttc tttagtttat tttcagaaaa agatacacaa aatttatatt
       61 tggcattttc ttgtctctcc gacagactga ccgaattgtt atcaatgtta acaaaaaaag
      121 aacaattggt tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt
      181 ccttctcgct ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctggatgta
      241 gaggtttatc actggaatct gcaaaacttc gccccggaag atctgcttta cgctcgtttc
      301 gatagtccgg cactcaagac cttcaccgaa cagctgcaac aggctgatgg actgatagtc
      361 gctacacctg tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg
      421 ccagagcgcg ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg tacggtggct
      481 catctgctgg cggtcgatta tgcccttaag ccagttttaa gtgcgatgaa agcccaggag
      541 ctcctgcacg gcgtgtttgc cgatgactca caagttattg attaccatca caaaccccag
      601 ttcacgccaa atctgcaaac ccgtcttgat accgcgctag aaactttctg gcaggcattg
      661 caccgccgcg atgttcaggt tcctgacctt ctgtctctgc gaggtaatgt ccatgcgtaa
      721 catcattaaa ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga
      781 atcctcgcct gaagcgttac gtataggcta tcagaaaggc agtattggta tggtattggc
      841 aaaaagccac cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt
      901 ccccgcgggt ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac
      961 cggggatatt ccgccaatct ttgcccaggc tgccggggct gatttgatat atgtgggcgt
     1021 cgagccaccg aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac
     1081 cgtagccgat cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct
     1141 tttactgcgt gcactgcgtc aggccggact taagtttact gacatccagc ccacttatct
     1201 gacgccagct gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg
     1261 ggatccctac tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac
     1321 cgatctcaat caaactggat cgttttatct ggcagctcgc ccctatgcag aaaaaaacgg
     1381 cgcttttatt cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca
     1441 gcgcgagcaa agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc
     1501 ctcgtatctg gatcatcgtc ctcccaccac catcaaaccg gtgagcgctg aagttgccgc
     1561 cttacagcag caaacggcag atctgtttta tgaaaaccgt ctggtgtcga aaaaagttga
     1621 tattcgccag cgcatctggc agcccactca cctggaagga aaacaattat gagtctgaat
     1681 atgttctggt ttttaccgac ccatggtgac gggcattatc ttggaacgga agaaggttca
     1741 cgcccggttg atcacggcta tctgcaacaa attgcgcaag cggcggatcg tctgggctat
     1801 accggtgtgc tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt ggcggcgtcg
     1861 atgatcccgg tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca
     1921 cctaccgttg ccgctcgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg
     1981 tttaatctgg tcacaggcag cgatccacaa gagttggcag gcgacggcgt gttccttgat
     2041 catagcgagc gctacgaagc ctcggcggaa tttacccagg tctggcgggg cgcttattgc
     2101 tgggggaaag cgtcgatttc aacggtaaac atattcatgt gcggggagca aaactgctct
     2161 tcccgccgat tcaacagccg tatcctccgc tttactttgg cggatcgtca gatgtcgccc
     2221 aggagctggc ggcagaacaa gttgatctct acctcacctg gggcgaaccg ccagagctgg
     2281 taaaagagaa gatcgaacaa gtgcgggcga aagctgccgc gcatggacgc aaaattcgtt
     2341 tcggtattcg tctgcatgtg attgttcgtg aaactaacga cgaagcgtgg caggccgccg
     2401 agcggttaat ctcgcatctt gatgatgaaa ctatcgccaa agcacaggcc gcattcgccc
     2461 ggacggattc cgtaggacaa cagcgaatgg cggcgctaca taacggcaag cgcgacaatc
     2521 tggagatcag ccccaattta tgggcgggcg ttggcttagt gcgcggcggt gccggaacgg
     2581 cgctggtggg cgatggtcct acggtcgccg cgcgaatcaa cgaatacgcc gcgcttggca
     2641 tcgacagctt tgtgctttcg ggctacccgc atctggaaga agcgtatcgg gttggcgagt
     2701 tgctgttccc acatctggat gtcgccattc cggaaattcc ccagccgcag ccgctgaatc
     2761 cgcaaggcga agcggtggcg aatgatttta tcccccgtaa agtcgcgcaa agctaaggaa
     2821 cacgatgatg gcaacgccag tgaagaagtg gttattgcgc gttgccccct ggtttttacc
     2881 ggtgggcatc gtggcggtgt ggcaactggc ctcctcggtt ggctggctgt cgacgcgtat
     2941 tttgccttca ccggaaggag tggtgacggc gttctggacg ctctccgcca gcggcgaatt
     3001 gtggcagcat ctggcgatca gttcctggcg ggcgctgatt ggcttttcaa ttggcggatc
     3061 gctgggcttg attctgggac tgattagcgg gctgtcacgc tggggagaac ggctgctgga
     3121 tacctcaatt cagatgttgc gcaacgtacc gcatctggcg ctgatcccgc tggtgatttt
     3181 gtggtttggc attgatgaat ccgcaaaaat ctttctggtg gcgctcggca cgctattccc
     3241 catttatatc aacacctggc atggcatccg taatatcgat cgcggtctgg tggaaatggc
     3301 gcgtagctat ggattatccg gcataccgct gtttatccat gtgatcctgc ctggtgccct
     3361 gccctcaatt atggtcggcg tgcgttttgc gttgggcctg atgtggctga cgctgattgt
     3421 tgccgaaacc atttctgcca attcaggcat tggttatctg gcgatgaatg cgcgggagtt
     3481 tttgcaaacg gacgtggtgg tagtcgctat tattctttac gccctgctcg gcaaacttgc
     3541 tgacgtcagc gcgcagctgt tagagcgcct ctggttgcgc tggaacccgg cttatcattt
     3601 gaaggaggcc acggtatgaa tactgctcgt ctgaaccagg gcacaccatt gttgctcaat
     3661 gcagtaagca aacattacgc ggaaaatatc gtcctgaacc aactggattt acatattccg
     3721 gcaggtcagt ttgtggcggt ggtgggccgc agcggtggtg gcaaaagtac cctgctgcgc
     3781 ctgctggcag gtctggaaac gccaaccgca ggcgatgtgt tagcgggcac cacaccgctg
     3841 gctgagattc aggaagacac gcgaatgatg tttcaggatg cgcgtctgct gccgtggaaa
     3901 tcggtgattg ataacgttgg gttaggcctt aaaggccagt ggcgcgatgc cgcgcgtcgg
     3961 gcgttggctg cagtaggact ggagaatcgc gccggggaat ggcctgccgc actttctggc
     4021 gggcagaaac agcgtgtggc gctggcaaga gcgttgattc atcgaccggg tttattgttg
     4081 cttgatgaac cgctcggggc actggacgcc ttaacgcgac tcgagatgca ggatttgatt
     4141 gtgtcacttt ggcagcagca cggctttacc gtactgttgg tgacgcatga tgtgagcgaa
     4201 gcggtagcaa tggctgaccg ggtgttgtta attgaagagg gaaaaattgg tctggatttg
     4261 acagtggata tcccccgtcc gcgtcgatta gggtcggtgc gattagcgga gctggaagcg
     4321 gaagtattgc aacgggtaat gcggcgtggg cattctgaac aactgattcg gcgtcatggt
     4381 taatatgccg gatgtggcgc acaaaaccag cattttcaac ggacttgtag gcctgataag
     4441 acacgtcaag catcgcatca ggcagttggg cacggctgcc ggatgcggcg tgaacgcctt
     4501 atccagccta caaaaccagc aatttcaacg gagttgtagg cctgataaga cgcgtcaagc
     4561 gtcgcatcag gcattttggc tcaaaagtat caagccagtg ctttggtgat cttctcgtac
     4621 agatcgccag agagattttc cagccctttc aactgttcca gcgccgcgcg cattttctcc
     4681 tgacgtttgg catcataacg tttcaggcga atcagcggtt caatcagacg tgaagctacc
     4741 tgcgggttac ggctgttgag gtcggtaagc atttcctcca ggaactggta accgctgcca
     4801 tcttcggcat ggaacgctgc cgggttgctg cccgcaaacg caccaatcaa cgaacgaata
     4861 cggttcgggt tgctcatggt aaatgagcga tgctgcaaca gaccacgcac cgtctccagc
     4921 acattcgccg ccgggctggt ggcttgcagg ataaaccatt tatccatcac cagaccgtcc
     4981 tgatgccact tatcgtcgta ctcctgcatc agcgcgtcac ggcaaggcag ctgtgcagca
     5041 accgccgcag aaagcgctgc cagcgcatcg gtcatattgt ttgcttcatg gtattgcttg
     5101 ctcaccagca cgtcagccag atgcgtttca ccaaaagcaa ggaagcgcag gcaggcattt
     5161 cgcagagtgc gttttgcaat atcttcatgc tcaacacggt actcgctctg gtaattcgcg
     5221 ttgtaaatag ccagcagctc atccgccagt tcagtcgcca gagtacgagt gagtgcttcg
     5281 cgtacttcgg caatagcaat cgggtcgatg atatcgaaca gttcagccat ttcattgaca
     5341 gaaggcagcg tcaggatttc tgccgccagc gccggatcaa tcttctcatc gagcagcacc
     5401 gcgcggaaag cgtcagccac atgtaccggc agagacagcg gctgcccttg ctgatgacgg
     5461 gcgacgttca gcttgatgta ggttgccagc aaactttgcg ccgcatccca acgggagaaa
     5521 tcattacgcg catgacgcat caggaaggtc agttgctgat cactccactt atattccagt
     5581 ttcactggcg cagagaattc gcacagcagc gcaggcaccg gctggaagta gacattatca
     5641 aaaacaaagg tctgttccgc ctgggtgacg ttcagcacgg aattcaccgg atgaccgcct
     5701 ttctgcaacg ggatcacttt gccttcgttg tcatacaatt cgatggcaaa cggaatatgc
     5761 agcggctgtt tttctgcctg atccggcgtg gctggcgtgc gctggctgat ggtcagggtg
     5821 tactgctcag tttccggatt gtagtcgtct ttgacggtca caatcggcgt accggactgg
     5881 ctgtaccaac ggcggaaatg ggagagatca acattcgacg catcttccat cgcctgcaca
     5941 aagtcgtcac aggtcgctgc gctaccatca tgacgctcga aatagagttg catccctttc
     6001 tggaagtttt cttcgcccag cagggtgtgg atcatacgga tcacttccgc gcccttctcg
     6061 taaacggtca gggtgtagaa gttgttcatc tcaatgacca tgtccgggcg gattgggtgc
     6121 gccatcgggc tggcgtcttc cgcaaactgc aatccgcgca tggtgcgcac attattgatg
     6181 cggttaactg cgcgggaacc aaggtcagag ctgaattcct gatcgcggaa gacggttaaa
     6241 ccttctttca ggctgagctg gaaccagtcg cggcaggtca ctcggttacc ggtccagtta
     6301 tggaaatatt catggccgat aacgcgttca atatcgaggt aatctttgtc ggtggcggtg
     6361 tcggtgcggg ccagcacata tttggagtta aagatattca gacccttatt ctccattgcg
     6421 cccatattga agaaatccac cgcgacgatc atatagatgt cgaggtcata ctccaggccg
     6481 aagcgttctt catcccattt cattgagttt ttcagcgagg tcatcgccca cggcgcgcga
     6541 tcaaggttgc cgcgatcgac gtacagctcc agcgccactt cgcgaccaga acgcgtggta
     6601 aaggtgtcgc gcagtacatc aaagtcgcct gccaccagcg caaacaggta gcacggtttc
     6661 gggaacgggt cctgccactg tacccaatgg cgtccgtttt ccagttcacc ttgcgcaacg
     6721 cggttaccgt tggaaagcag gaagggatat ttggttttat cggcaataat tttggtggta
     6781 aaacgcgcca gcacgtccgg gcggtcgaga taatacgtaa tatggcggaa accttcggct
     6841 tcacactggg tgcaaagcgc atcgcctgac tgataaagcc cttccagcgc ggtattcgcc
     6901 gccgggctta tttcattaac gatcttaagc gtaaaatgct ccggcaaatt actgatgacc
     6961 agtgcgccct cttcttcttt ccaggcggtc cacggctcat cattaatatg aacagaaacc
     7021 agtttgaggt cttcgccgtt gagacgaagt ggagcatctg atgcaccatg acggacagcc
     7081 tggctgaccg cggtaacgac cgtcttttgc gcgtcgaggt caaaggtcaa gtcaatatca
     7141 gtaatctggt aatctggcgc acgataatcg tgacggtatt tggcttgtgg ctgttgagtc
     7201 ataaataacc tttagcatct tttatagagt ctggtgttca gtctattcct gttgcgtaaa
     7261 tcgcgctatg cagaatcttc atcttttcag gtacaaacgc ctttattgct acatttttat
     7321 aacatacacc gcgtaatgcc atcgaccaga aaggtggcat atggtgtgat cggggttcaa
     7381 taaattgcga aacaaggtat actccagcag ttcctgaaga tgtttattgt actaaacgct
     7441 cctgtacgag gacgctactg cgcacctatg acacaattcg cttctcctgt tctgcactcg
     7501 ttgctggata cagatgctta taagttgcat atgcagcaag ccgtgtttca tcactattac
     7561 gatgtgcatg tcgcggcgga gtttcgttgc cgaggtgacg atctgctggg tatttatgcc
     7621 gatgctattc gtgaacagat tcaggcgatg cagcacctgc gcctgcagga tgatgaatat
     7681 cagtggctct ctgccctgcc tttctttaag gccgactatc ttaactggtt acgcgagttc
     7741 cgctttaacc cggaacaagt caccgtgtcc aacgataatg gcaagctgga tattcgctta
     7801 agcggtccgt ggcgtgaagt catcctctgg gaagttcctt tgctggcggt tatcagtgaa
     7861 atggtacatc gctatcgctc accgcaggcc gacgttgcgc aagccctcga cacgctggaa
     7921 agcaaattag tcgacttctc ggcgttaacc gccggtcttg atatgtcgcg cttccatctg
     7981 atggattttg gcacccgtcg ccgtttttct cgcgaagtac aagaaaccat cgttaagcgt
     8041 ctgcaacagg aatcctggtt cgtgggcacc agcaactacg atctggcgcg tcggctttcc
     8101 ctcacgccga tgggaacaca ggcacacgaa tggttccagg cacatcagca aatcagcccg
     8161 gatctagcca acagccagcg agctgcactt gctgcctggc tggaagagta tcccgaccaa
     8221 cttggcattg cattaaccga ctgcatcact atggatgctt tcctgcgtga tttcggtgtc
     8281 gagttcgcta gtcggtatca gggcctgcgt catgactctg gcgacccggt tgaatggggt
     8341 gaaaaagcca ttgcacatta tgaaaagctg ggaattgatc cacagagtaa aacgctggtt
     8401 ttctctgaca atctggattt acgcaaagcg gttgagctat accgccactt ctcttcccgc
     8461 gtgcaattaa gttttggtat tgggactcgc ctgacctgcg atatccccca ggtaaaaccc
     8521 ctgaatattg tgattaagtt ggtagagtgt aacggtaaac cggtggcgaa actttctgac
     8581 agccctggca aaactatctg ccatgataaa gcgtttgttc gggcgctgcg caaagcgttc
     8641 gaccttccgc atattaaaaa agccagttaa tatcatcagg gagctaatcg gctccctttt
     8701 tttaccttta attccgaaat ctttcgctgc atttgcgaat tctgcttgtc tgattgcaga
     8761 tgccaggtaa cataggtatc ccccccccca tttacgggtg gacaagtgtt tattttttcc
     8821 gactattaac agagagaata ttatgagcgt tgtgcctgta gccgacgtac tccagggccg
     8881 cgtagccgtt gacagcgaag tcaccgtacg cggatgggta cgtacccgcc gagattcaaa
     8941 agctggcatc tccttcctcg ccgtttatga cggttcctgc tttgatcctg tacaggctgt
     9001 catcaataat tctctgccca attacaatga agacgtcctg cgtctgacca ccggctgctc
     9061 ggtcattgtg acgggtaaag tcgtggcatc gccgggccag gggcaacaat ttgaaattca
     9121 ggccagcaag gttgaagttg ctggttgggt tgaagatcca gacacttacc cgatggcggc
     9181 aaaacgccac agcattgagt atctgcgtga agtcgctcac ctgcgtccgc gcacaaacct
     9241 gattggtgcc gtcgcgcgcg ttcgccatac gctggcgcag gcgctgcatc gcttctttaa
     9301 cgagcaggga ttcttctggg tttcaacgcc actgattacc gcatctgata ccgaaggtgc
     9361 aggcgaaatg ttccgcgttt ctacgctgga tctggaaaac ctgccgcgta acgatcaggg
     9421 caaagtggat ttcgacaaag acttctttgg taaagagtct ttcctgaccg tatctggcca
     9481 gttgaacggc gaaacctacg cttgcgcatt atccaaaatt tataccttcg gcccgacttt
     9541 ccgtgctgaa aactccaaca ccagccgtca cctggcggaa ttctggatgc tggagccgga
     9601 agtggcgttt gctaacctga acgatatcgc gggtctggct gaagccatgc tgaaatatgt
     9661 cttcaaagcg gttctcgaag aacgcgcaga cgacatgaaa ttcttcgctg aacgcgtaga
     9721 taaagatgcc gtttcacgtc tggaacgctt cattgaggcc gatttcgcgc aggtggatta
     9781 caccgacgca gtgaccattc tcgaaaactg cggcaggaag tttgaaaacc cggtttactg
     9841 gggcgtcgat ctctcttctg agcatgagcg ttatctggcg gaagaacact ttaaagcacc
     9901 ggtagtggtt aaaaactatc cgaaagatat taaagcgttc tatatgcgcc tcaacgaaga
     9961 cggtaaaacc gttgcggcta tggacgttct ggctccgggc atcggtgaga tcattggtgg
    10021 ctcccagcgt gaagaacgtc tggacgttct ggacgagcgt atgctggaaa tgggcctgaa
    10081 taaagaagat tactggtggt atcgcgatct gcgtcgctac ggtactgttc cgcattcagg
    10141 tttcggtctt ggttttgaac gcctgattgc ttacgtaact ggcgtgcaaa acgtgcgtga
    10201 tgtgattccg ttcccacgta ctccgcgcaa cgccagcttc tgattttctt cctcaaagag
    10261 ccagcgcctg ctggctcttt tttatccttt ctgtcaagtt atctgtttgt taagtcaagc
    10321 aatctatttg caaccccgcc ataacgatca tcctgttacg gaatattaca ttgcaacatt
    10381 tacgcacaaa aaataacccg cattcttatt gcggattagt tttttcttag ctgatagcac
    10441 aattttcata ctattttttg gcattctgga tgt
//
LOCUS       DAZPAC010000142        10420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZPAC010000142 DAZPAC010000000
VERSION     DAZPAC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.142"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACHUPM_003011"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACHUPM_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR0870724.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUPM_003012"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUPM_003012"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR0870725.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHUPM_003013"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHUPM_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFR0870726.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHUPM_003014"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHUPM_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR0870727.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHUPM_003015"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHUPM_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHUPM_003016"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHUPM_003016"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR0870728.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVTAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHUPM_003017"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHUPM_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR0870729.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHUPM_003018"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHUPM_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR0870730.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="ACHUPM_003019"
     CDS             complement(9457..10149)
                     /locus_tag="ACHUPM_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR0870731.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2549 a         2514 c         2752 g         2605 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tgactgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc
//
LOCUS       DAZPAC010000143        10253 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZPAC010000143 DAZPAC010000000
VERSION     DAZPAC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10253
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.143"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            176..679
                     /locus_tag="ACHUPM_003020"
     CDS             176..679
                     /locus_tag="ACHUPM_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR0870732.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(758..1009)
                     /gene="yecJ"
                     /locus_tag="ACHUPM_003021"
     CDS             complement(758..1009)
                     /gene="yecJ"
                     /locus_tag="ACHUPM_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR0870733.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1124..1210)
                     /gene="azuC"
                     /locus_tag="ACHUPM_003022"
     CDS             complement(1124..1210)
                     /gene="azuC"
                     /locus_tag="ACHUPM_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR0870734.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1473..1796
                     /locus_tag="ACHUPM_003023"
     CDS             1473..1796
                     /locus_tag="ACHUPM_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR0870735.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1967..2464
                     /gene="ftnA"
                     /locus_tag="ACHUPM_003024"
     CDS             1967..2464
                     /gene="ftnA"
                     /locus_tag="ACHUPM_003024"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR0870736.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2502..2741)
                     /locus_tag="ACHUPM_003025"
     CDS             complement(2502..2741)
                     /locus_tag="ACHUPM_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR0870737.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            2932..4143
                     /gene="tyrP"
                     /locus_tag="ACHUPM_003026"
     CDS             2932..4143
                     /gene="tyrP"
                     /locus_tag="ACHUPM_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR0870738.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4205..4870)
                     /gene="yecA"
                     /locus_tag="ACHUPM_003027"
     CDS             complement(4205..4870)
                     /gene="yecA"
                     /locus_tag="ACHUPM_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR0870739.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5066..5152)
                     /locus_tag="ACHUPM_003028"
     tRNA            complement(5066..5152)
                     /locus_tag="ACHUPM_003028"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5116..5118),aa:Leu,seq:taa)
     gene            complement(5165..5238)
                     /locus_tag="ACHUPM_003029"
     tRNA            complement(5165..5238)
                     /locus_tag="ACHUPM_003029"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5204..5206),aa:Cys,seq:gca)
     gene            complement(5293..5368)
                     /locus_tag="ACHUPM_003030"
     tRNA            complement(5293..5368)
                     /locus_tag="ACHUPM_003030"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5333..5335),aa:Gly,seq:gcc)
     gene            complement(5520..6068)
                     /gene="pgsA"
                     /locus_tag="ACHUPM_003031"
     CDS             complement(5520..6068)
                     /gene="pgsA"
                     /locus_tag="ACHUPM_003031"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR0870740.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6125..7957)
                     /gene="uvrC"
                     /locus_tag="ACHUPM_003032"
     CDS             complement(6125..7957)
                     /gene="uvrC"
                     /locus_tag="ACHUPM_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR0870741.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7954..8610)
                     /gene="uvrY"
                     /locus_tag="ACHUPM_003033"
     CDS             complement(7954..8610)
                     /gene="uvrY"
                     /locus_tag="ACHUPM_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR0870742.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8906..9082
                     /gene="yecU"
                     /locus_tag="ACHUPM_003034"
     CDS             8906..9082
                     /gene="yecU"
                     /locus_tag="ACHUPM_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR0870743.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9069..9293
                     /gene="yecF"
                     /locus_tag="ACHUPM_003035"
     CDS             9069..9293
                     /gene="yecF"
                     /locus_tag="ACHUPM_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR0870744.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9360..10082)
                     /gene="sdiA"
                     /locus_tag="ACHUPM_003036"
     CDS             complement(9360..10082)
                     /gene="sdiA"
                     /locus_tag="ACHUPM_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFR0870745.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2588 a         2552 c         2352 g         2761 t
ORIGIN      
        1 atgatttaag cattttctta tacccgttca gacgttattc ttatttcaga tcatcgtcag
       61 aattgactcc acgatcacat ttcggaccgg cagaaaggaa ttattctgca aacagtaatt
      121 atggtgtttt gatttatctt gcacctctcc acttctggat ataaggatat taggtatggc
      181 aaccgctgga atgcttctca aactcaactc tcaaatgaac cgcgagtttt acgcatccaa
      241 tctctacctt cacctgagta actggtgttc tgaacagagt ctgaacggca ccgccacttt
      301 ccttcgcgcc caggcacaga gtaatgtgac ccaaatgatg cgcatgttta actttatgaa
      361 gagtgtcggc gctaccccca tcgttaaagc cattgatgtt cccggtgaaa aactgaactc
      421 tctggaagaa ctgttccaaa aaacgatgga agaatacgag caacgttcta gtacgttggc
      481 acagttagcc gatgaagcga aagaactgaa tgatgattca accgtcaatt tcctgcgcga
      541 tctggaaaaa gaacagcagc atgatggtct gttgctgcaa accattcttg atgaagtgcg
      601 cagtgcgaaa cttgcgggta tgtgccctgt gcagaccgac caacatgttc tgaatgtcgt
      661 gtcacaccag ctgcattgat catcatcggc gctaatgcat tgcgccgatg aaggttttga
      721 gaaaccgctg cctcatctgt ttgaagcagc ggttttttta atgggattca ccctgtgggg
      781 taaacttgag ttcaataagc gcgatggctt tttggattgc ccgcatggtg accgggtctg
      841 cggcggcggg atggttagta aagtcgatat tcttcagctg actggacatt ttttcacgaa
      901 cttcaacggg cgcgattaca tcgagaacat ccagaatttg tttgataacc aactggcaag
      961 caaccacatc agaaaccagt tcctgatcgg cattcagcgg ctgggacatc gtaaactcct
     1021 gatagcattt tgaaagccgt tatagtagcg gcttcacatc ttcagcgata gtcacatcca
     1081 ccgtcatcag gacacaaaaa aacctgccgg agcaggtttt ttgttatcgg aacatattgc
     1141 ctggcggtac gtctttgaac gtcttgcaat agttattgaa catacttttc aggattttgc
     1201 gcagtttcat cgcggcactc cgaccatttg ttatacaggt gttattgtct ttgcgcttat
     1261 aatatgacaa ccatcacaaa aatcaatctt tatgtgatac aaatcacata aatacccctt
     1321 taatgttata aaaatgataa tcaaaaaaca gcccccctat ttctgacacc tacagatggc
     1381 aagaaatagc gcctgccagg cgtcttttcc ggccattgtc gcagcgctgt aacgcgtaaa
     1441 atagtgcttt ctcttactct tctggctgga ccatgagact tctgattctg actctttcat
     1501 taataacgct ggcggggtgt acggttactc gccaagccca tgtgagcgaa gttgacgcgg
     1561 caacaggtat tgtacggttg gtttatgacc aggctttttt gcagcatgcc cataccgatc
     1621 gttatgtcag tcgcgggatc gccgatcgcg catgccagca ggaaggctat acccacgcgg
     1681 tcccctttgg tcagccagta ggcaactgta gcctttttgc gggttcctta tgtttgaata
     1741 cggaattcac tttgtcatat cagtgccatc attccgcctt ccctgtcttt ctctaaatat
     1801 tttaatcagc gaggggatct tcgctgatta aagaaatagc ggtaatgcgt tttattccca
     1861 ttcgtatttt taataattga agtttatatt ttaccttttg caaataataa aataacaaat
     1921 tatagtgacg ccacagcaac aaatataacc tttgtggagc actatcatgc tgaaaccaga
     1981 aatgattgaa aaacttaatg agcagatgaa cctggaactg tactcttcac tgctttatca
     2041 gcaaatgagc gcctggtgca gctatcatac cttcgaaggt gctgccgcgt tcctgcgccg
     2101 tcacgcccag gaagagatga cgcatatgca gcgtctgttt gattacctga ctgataccgg
     2161 caatttaccg cgtattaata ccgttgaatc tccgtttgct gaatattcct cacttgatga
     2221 attattccag gaaacctata aacacgaaca attaatcacc cagaaaatta acgaactggc
     2281 tcatgctgca atgaccaatc aggactaccc aacatttaat ttcctgcagt ggtatgtttc
     2341 tgagcagcat gaagaagaga aactgttcaa atcgattatt gataaattaa gcctggcagg
     2401 caaaagcggc gaaggtctgt attttatcga caaagaactc tctaccctcg acacacaaaa
     2461 ctaatgctct ccggcggcag gtttactgcc gccgtttcaa atcagtgacg gcaaatctta
     2521 ctctgatcgg tcgaaaaacc gtctttcgaa ggtataaatt tgccttttgc tgccagaaac
     2581 gctaccagct ctcccgctgt catcccttct gccgagcagg tgtgaaaacg tgcctgttca
     2641 ccaaaacgcg ctttaatcgc agcttccaga ctggcatgcg tatattgctc gcctgattca
     2701 atcatcatat ttaacacttc atgaccgtga atagagtcca tcgttcctcc tcaaaaaaag
     2761 cctagcgtag cgattgccgc ttatgaagac tttgcgccag cgcaggactg aatgcttttt
     2821 attgtacatt tatatttaca ctatatgtaa cgtcggtttg acgaagcagc cgttatgcct
     2881 taacctgcgc cgcagatatc actcataaag atcgtcagga cagaagaaag cgtgaaaaac
     2941 agaaccctgg gaagtgtttt tatcgtggcg ggaaccacaa ttggcgcagg catgctggca
     3001 atgccgctgg ctgcggccgg tgttggtttt agcgttacgt taatcttgtt gattgggctt
     3061 tgggcgttga tgtgctacac ggcgctatta ctgctggagg tgtaccagca tgttccggca
     3121 gataccggtc tgggcacgct ggcaaaacgc tatctgggac gctacggtca atggctgacg
     3181 ggcttcagta tgatgttctt aatgtatgct ctgactgcgg catacatcag cggtgtcggt
     3241 gaattgttgg cctccagcat cagcgactgg acaggtattt caatgtcggc aaccgctggc
     3301 gtgctgttgt tcacttttgt tgccggtggc gtggtttgtg ttggaacatc gctggtcgat
     3361 ttatttaacc gttttctgtt cagcgccaaa attatttttc tggtggtgat gctggtattg
     3421 ctgctaccgc atattcacaa agtgaatctt ttaaccctgc cgttgcaaca ggggctggct
     3481 ctgtcagcaa tcccggtgat ttttacctca tttggttttc acggtagcgt gccgagtatt
     3541 gtcagctata tggatggcaa cgttcgtaag ctacgctggg tgtttataac cggtagtgcg
     3601 atccccctgg tggcatatat tttctggcag gtggcaacgc ttggcagcat tgattcaaca
     3661 acctttatgg gattgctggc taatcatgct ggattaaacg ggctgttaca ggcgttacgc
     3721 gaaatggtgg cctctccgca tgttgagctg gcagtgcatt tatttgctga tttagccctc
     3781 gccacgtcat ttctcggcgt tgcgttaggc ttatttgatt atctggctga tttatttcag
     3841 cgttcaaata ccgttggtgg acggttgcaa actggtgcaa ttacctttct gccgccgttg
     3901 gcgtttgcac tgttttatcc acgaggattt gtgatggcgc tgggttacgc cggtgtggcg
     3961 ctggcggtac tggcattgat tatcccttcg ctgttgacct ggcaaagcag aaagcacaat
     4021 cctcaggcgg gttaccgggt caaaggtggt cgtccggcgc tggtggtggt gtttctctgt
     4081 ggtattgctg tgattggcgt gcaatttttg attgcggcag ggttgttacc agaagtgggg
     4141 tgatcagata gcctcaaatt ccttattggg tgccagaatt aacgctgaca cccaatttgg
     4201 cctcttaatg caggcagcac tgcttaaatt tcttaccact accgcacggg caaggatcgt
     4261 tacgccccgg tttctcttct gctttgatcg gttgctgaac agctttttcc tgcggatgcg
     4321 ccatccagta cgcatgtaga tcaagcgccg ccagtcgaat ggcatctacg ctctcttcaa
     4381 acgcttctgg cgacatcttt tctacccgct cgaagttttc ctcagtaccg tgcagcgcaa
     4441 tcgcctccag cgctggtttt aacgaatcgg gcaacgttga ccagtcagaa agtgccacgc
     4501 cccgcatata gccaaagcac cactcctcaa caatcgtcag ctcgctgcca tcaacttctc
     4561 gcaagccgaa taacggctca aactgctccg ggaattcgtt cagacgctct gcggtatcgg
     4621 ccatatgttg aaaagccagg ttcataaagc gcgtcatctc tttctctgac gcccagcgcg
     4681 gcacatagtc agccccaccc cacacggcaa ccagccactg ttccggttca atctcttgcg
     4741 gagaactcaa caccgccgtc aataaaccgt ccagctccgc cacatcaagg atggcgtggt
     4801 tagtgttgta tttggtcaga atatcgtcca gccattccaa ctcactttcg tttaacggtc
     4861 ccgttttcat acgcttttcc ttgtggatct caactcgcca gcacctatct tacatgccgg
     4921 tccgtatcag agatactttt tgagtggctt tgccggtgat taaaaattaa ggagggtgta
     4981 acgacaagtt gcaggcacaa aaaaaccacc cgaaggtggt ttcacgacac tgcttattgc
     5041 tttgatttta ttcttatctt tcccatggta cccggagcgg gacttgaacc cgcacagcgc
     5101 gaacgccgag ggattttaaa tcccttgtgt ctaccgattc caccatccgg gctcgggaag
     5161 aaagtggagg cgcgttccgg agtcgaaccg gactagacgg atttgcaatc cgctacataa
     5221 ccgctttgtt aacgcgccaa attcttcagg cctttcagcc agacatccgc ttgacgccga
     5281 tgtcttttaa actggagcgg gaaacgagac tcgaactcgc gaccccgacc ttggcaaggt
     5341 cgtgctctac caactgagct attcccgcat tcatcaagca atcagttaat cacttgattt
     5401 tattatcgtc tggcaatcag tgccgccgtt cgatgcgttg cattctactt acctggcgcg
     5461 atgagtcaac gatatttttc accacttttg atcgtttgct gaaaattacg ccgaaacgat
     5521 cactgatcaa gcaaatctgc acgcgcagcg ctcaaatatt gcaacattga ccacagagtc
     5581 agtaccgcag ccacaaagaa aagtgcaata ccggcgtact caacccaaat gttcggacgc
     5641 cacagcagcc atgccaacgc caccatctgg gcagtggttt tcactttccc aatccaggag
     5701 acagccacgc tactgcgttt acccaactcc gccatccatt cgcgtagcgc agaaataata
     5761 atttcacggg cgatcatcgt tgctgccggt aaggtcaccc accagctgtg gtaatgctcg
     5821 gttaccagca ccatggcgat agccacgaga actttatctg ctacagggtc aaggaaagca
     5881 ccaaaccggg tactctggtt ccagcggcgt gccagaaaac catcgaacca gtcagtcacc
     5941 gccgcgacgc agaaaatgag cgcggcggca aacggcgacc aggtgacagg cagataaaag
     6001 accaatacaa agaatgggat aaggatgaca cggaacagtg taagcaacgt agggatatta
     6061 aattgcataa tgacgggtaa ctatctgttg tcagtaagat tacccctatg ttgctacaga
     6121 gacatcaatg tttcaacgac cagaagatct tttctgccag accttgcgaa atacccggca
     6181 cttttgcaat ttcctcgacg ctggcgttac gtaaaccttg caaaccgccc atatatttca
     6241 acaacatttg ccgacgtttt ggcccgacgc cttcaatggt ttccagggaa ctggtatttt
     6301 tgaccttcgc ccgtttttta cggtgcccgc caatcgcgtg atcatgtgat tcatcgcgaa
     6361 tatgctggat aacatgcagc gcgggagaat ctggcggcaa actgaatccc tcaccttccg
     6421 gctcaaagaa cagcgtttcc agcccagcct tacgatctgc tcctttggca acgccaagta
     6481 gcagcggatg atttttatcc catgagacat ccagttcggc gaagacattt ttcgcctgcg
     6541 caagctggcc tttgccgccg tcgataagga tcacatccgg gatcttactg tcgtcaatgg
     6601 ctttaccata acgccgacgc agcacctgat tcatcgccgc ataatcatcg cccggcgtga
     6661 tgcctgtaat gttatagcgc cgatactccg cacgcagcgg gccgttagca tcaaacacca
     6721 cacaggaagc gacggtttgt tcgcccatgg tatggctgat gtcaaagcac tccatccgct
     6781 tcacttccgg caatttcaac acgctggcaa gcgcggtcag tcgctggtga acggtagatt
     6841 gctgcgaaag tttgctggtt aaggccgtcg ccgcattggt gcgcgcgagt ttcagataac
     6901 gcgccctatc gccgcgaggt ttggtttgaa cattaatctt gcgtcccgcc agttctgaaa
     6961 gggaatcggc gagcagcgtt ttatcgctaa gattaaaatc gagcaggatc tcacccggta
     7021 aggtgcgcat ctggctgcct tgtaaataga actggcctac gaaggtttct accacctcgc
     7081 tcagttccgt accgccaggc actttcggga aatagctgcg gctgccgagc actttgccct
     7141 gacgaatgaa caatacgtgg acacaagcca tgcccgcatc gaacgccaca ccaataacgt
     7201 cgaggtcgtc gccggtattg gaaacgaatt gtttttcggt gacgcgtcgc accgcctgaa
     7261 tttggtcgcg aatacgtgca gcttcttcaa actccagatt ctggctggca gtttccatac
     7321 gactaatgag ttgcgtaagc acctgatcat ctttgccaga caaaaacagg cgcacatact
     7381 caacctgctg agcgtattct tcttcactca ccagtccttc aacgcacggt cccagacagc
     7441 gccctatctg atattgcaga cacggacgcg agcgattgcg ataaacacta ttttcgcact
     7501 ggcgaatggg gaaaatcttt tgcagtagcg ccagtgtttc acgtacggca tagccattcg
     7561 ggaacgggcc gaaatattca cctttggcat gcttcgcacc acgatgcatc gccagacgcg
     7621 ggtgggtatc gccactcagg aagataaaag gataagattt atcatcgcgt agcaaaacgt
     7681 tgtaacgcgg ctgatagagt ttgatgtagt tgtgttccag caacagcgct tcggtttctg
     7741 tatgcgtaac cgttacatca atttgctgga tctgggcgac cagcgcttcg gttttgcgcg
     7801 aagcgaggtt gctgcggaaa tagctggaaa gccgtttttt caggtctttc gctttgccga
     7861 catagataac cgtaccacca gcatcgtaca tgcgataaac gcctggctgg caggttacgg
     7921 tttttaaaaa cgcttttgcg tcaaactgat cactcactga cttgataatg tctccgcatt
     7981 acacagacca tggcgaattg ccaggtgagt cagctcaaca tcgccatgaa tgtttagttt
     8041 actgaacata cgatagcggt agctgttcac cgttttcgga ctgagattga gctgttctga
     8101 gatctcattg accttctggc ccttggtgat catcagcata atctgcaatt cacgttcaga
     8161 caaactggca aatgggcttt ctgttttttc tggttcgatc tggcttaacg ccatttgttg
     8221 agcgatatca gaagcaatgt aacgctgccc tgaatagaca gaacgaatcg cactcacgac
     8281 ttcctgcgga gccgcgcctt tgctgaggta gcccgcagca ccggcctgca tgactttcgc
     8341 tggtaaaggg ttttctgtat ggacggtaag catgatgatt ttgacatcag ctgtggaacg
     8401 cgcgatttta cgcgtcgcct caagaccgcc aatgcccggc atactcatgt ccattagcac
     8461 cacgtcaacg gcatttgtcc ggcaccactt aacggcgtct tcaccgcacg atgcctcacc
     8521 gacgactttt atacccttta tatcttccag aatgcgtcgt atccctgcgc gcaccagttc
     8581 gtggtcatca acaagtagaa cgttgatcaa aggaatatct ccagaaatag ggataacgct
     8641 actgatagtt agtcattcgt atattagcgg tttttattgc aactttgaaa cgttaaaaat
     8701 ggtgggtatt cgatttttct ctcgtttttg taaattcgat tgtccacagt tatggtcacg
     8761 cccgtcacca acggcttccg ggatcgtttc acagtaagcc cggcgatgaa atgtgacaaa
     8821 aattgacttt attcagcaaa aatgaaaatc agcccgctga atatgtaaca ttaattaacc
     8881 cgaacaatac gggcaaaaaa cattcatgat aaaaatattt attggtcatt atattaacgc
     8941 atttaatagc attgttgacg tcacgctcaa aaaacaacca ctgctatttt tagcaaagct
     9001 tatggtatac tccgccgcct taacattttt caccgcaaat tttcattgca acatgacgag
     9061 gaaaataaat gagtacgcct gatttttcta ctgccgagaa taatcaagaa ctggcaaatg
     9121 aagtctcctg cctgaaagcg atgctgacgc tgatgctaca ggcgatggga caagctgacg
     9181 cgggccgcgt gatgttaaag atggaaaaac agcttgcgct gatcgaagac gaaacccagg
     9241 ctgcagtatt ttccaaaacg gttaagcaaa ttaaacaagc ctaccgtcag taatataaaa
     9301 ccggctgaga gcgtgccttt cagccgattt ttgcatctgg cacgcagaaa gaaaagcgat
     9361 caaattaagc cagtagcggc cgcgtaacag gccacctggg tcttatttgg tgcattgatt
     9421 tttttctgca tgtttttctg atggaaattg accgtattct cagagattga caaaatcatc
     9481 gctatctctg ctgatgtctt cccttccgcc gtccatttca gaatttcttt ttcgcgcttg
     9541 ctgaaattca tctctggcgt catcactatt tcatcattta aacgcatcag agccatcaga
     9601 ctttcgcgca ccagtaactg catttttaat tgcagttcat cactaagaat gggtatttcg
     9661 cgcgtgctgc aacgggaaaa ggacaaaaag cccagcgccc gatttggcag cattaaatac
     9721 tgagtgacac cgcggcgtaa accatgtgcg tgcgcggctt cccataatgg ctgtgcttcg
     9781 ctgaataagt catcattcca cattaaatgg ccctgactaa agttttcagg gttcagcaca
     9841 ggatcaattg cgagaaaatt ttttgcctga taataactaa cccacgactc agggtaattg
     9901 gtgtagaaag ccactttagg tcgcgtgaat ggtaccgggt ggcgaacaca taacgaatag
     9961 taatcgtact ccagctgctg agcctgaagc tctatttcat ggtagacctc ttctgcggcc
    10021 tccatcctct gaaaacgcaa cagcatcgtg cgacgccagc tgaaaaaatc cgtatcctgc
    10081 atagtaaacc gcaacgcccc tgagagtgag tatcatttat aatgataatt caaactatca
    10141 cataaataag atttatatat aatttatatt attcaggcaa tgaattactt ttgcaagcca
    10201 tcgcattctc ttatgttatt aatgagttat gctgatttgt taagcagttt tat
//
LOCUS       DAZPAC010000144        10135 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZPAC010000144 DAZPAC010000000
VERSION     DAZPAC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10135
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.144"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..611
                     /locus_tag="ACHUPM_003037"
                     /pseudo
     CDS             1..611
                     /locus_tag="ACHUPM_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            608..1222
                     /gene="prfH"
                     /locus_tag="ACHUPM_003038"
     CDS             608..1222
                     /gene="prfH"
                     /locus_tag="ACHUPM_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR0870746.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="ACHUPM_003039"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="ACHUPM_003039"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0870747.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="ACHUPM_003040"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="ACHUPM_003040"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0870748.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="ACHUPM_003041"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="ACHUPM_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR0870749.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="ACHUPM_003042"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="ACHUPM_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR0870750.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="ACHUPM_003043"
     CDS             complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="ACHUPM_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR0870751.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6632..7735
                     /gene="proB"
                     /locus_tag="ACHUPM_003044"
     CDS             6632..7735
                     /gene="proB"
                     /locus_tag="ACHUPM_003044"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR0870752.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGNSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7747..9000
                     /gene="proA"
                     /locus_tag="ACHUPM_003045"
     CDS             7747..9000
                     /gene="proA"
                     /locus_tag="ACHUPM_003045"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR0870753.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9115..9190
                     /locus_tag="ACHUPM_003046"
     tRNA            9115..9190
                     /locus_tag="ACHUPM_003046"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9148..9150,aa:Thr,seq:cgt)
     gene            9796..9954
                     /locus_tag="ACHUPM_003047"
     CDS             9796..9954
                     /locus_tag="ACHUPM_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870754.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2510 a         2456 c         2631 g         2538 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc
     3781 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa
     4021 gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcatcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc tctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga agcgcaggaa
     5041 tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagacactga agtggttgaa cgactggagc acaccctacg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat cacatccggc
     5281 aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaccga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact ggcgttatca ctcatataat gcatagcttt aactttgcca
     6241 tagacatcca gtttattacc gtctttatta tatatttctg cagcctgtac agatgcagat
     6301 gccacaatgc ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt
     6361 aattaacgcg cgaatattca gcgggagagc cccgttgaaa acaggaaagt ttttaacctg
     6421 agattgttaa agatatatta cagattaata atattcttaa aatgtggtaa tttattaaat
     6481 ctgtaataaa agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa caaaacgcca
     6541 tgctttgctc gcagatggtt ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat
     6601 catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc tggtggtaaa
     6661 actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc atattgttga
     6721 acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta ttgtgacgtc
     6781 gggcgcgatc gccgccggac gtgagcacct gggttacccg gaactgccag cgactatcgc
     6841 ctcgaaacaa ctgctggcag cggtagggca gagtcgactg attcaactgt gggaacagct
     6901 gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg atatggaaga
     6961 ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt
     7021 tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaagtcg gcgataacga
     7081 taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc tgctgaccga
     7141 tcaaaaaggt ttgtataccg ctgacccgcg cagcaatccg caggcagaac tgattaaaga
     7201 tgtttacggc attgatgacg cactgcgcgc gattgccggt aacagcgttt caggcctcgg
     7261 aactggcggc atgagtacca aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga
     7321 caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg aaggcatttc
     7381 cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac gctggatttt
     7441 cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg gcaactgccg ccattctgga
     7501 acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg
     7561 tgaagtcatc cgcatttgca acctcgaagg ccgcgatatc gcccacggcg tcagtcgtta
     7621 caacagcgat gcattacgcc gtattgccgg acaccactcg caagaaattg atgcaatact
     7681 gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc gttaaggagc
     7741 tggccgatgc tggaacaaat gggcattgct gcgaagcaag cctcgtataa attagcgcaa
     7801 ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa
     7861 agcgaaatca tcctcaacgc taacgcccag gatgttgctg acgcgcgagc caatggcctt
     7921 agcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg cattgccgac
     7981 gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc aggtaatcga tggcggcgta
     8041 ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt
     8101 tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat
     8161 gcggtgatcc tgcgcggtgg caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg
     8221 attcaggacg ccctgaaatc ctgcggctta ccggcgggtg ccgtgcaggc gattgataat
     8281 cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga catgctgatc
     8341 ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat cccggtgatc
     8401 acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgtagagat cgctgaagca
     8461 ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac ggttgaaacg
     8521 ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa acaaatggcg
     8581 gaaagcggcg tgacattaca cgcagatgca gctgcactgg cgcagttgca ggcaggccct
     8641 gcgaaggtgg ttgctgttaa agccgaagag tatgacgatg agtttctgtc attagatttg
     8701 aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga acacggcaca
     8761 caacactccg atgcgatcct gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa
     8821 gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt
     8881 ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg
     8941 ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat tcgtgcgtaa
     9001 ataaaaccgg gtgatgcaaa agtagccatt tgattcataa ggccattgac gcatcgcccg
     9061 gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt cagggccgat
     9121 atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt
     9181 atcggcacca ttaagaaaat taataactta gcttaacttc tctaagaaat tgtatcttct
     9241 gttatcactt acaaacgcct atacgttgtt atgggctttg gtagtgtaac acgtatgggc
     9301 gagtagttcc caggctcaac ctcgtaacaa gttcctttga agctggaaga cgtgccaccg
     9361 tctctaaacc ttctaccaaa actcttagca agatgttcca cacctggcgc ggtgtccaat
     9421 gatgctacac gtcgtgagac gttgcagagg ataaacaggc taaatgatgg gaagagtttt
     9481 aagtgcaata gtggattaaa gcccacctct tcaaaaccta acttgatcgg gaaagaatca
     9541 agctctagca cggtttcgtc caccggagta caacgggtgc ctgataagag aagaactgct
     9601 aagacttcat agcttttgtg aaaaagccct ttttagtgga tacgcctacg gcgcttattt
     9661 tttggtataa ccatcaaagg cgctttatgg ctcctattgt cttgaaaatg tatctttact
     9721 tgtattgtgt tttatgcgta gtctccctgt gtagtatgta acttgttaat tttcatatga
     9781 atacagaggc acacgatgaa aaacattgca gcaatcaaac gtaataaccg caagattcac
     9841 gctcgtaagt tcctgtctac gccagaagga aaagcctggc tagagcgtaa acagagagag
     9901 aacgaagaaa gaaaactcct tagtgagttg aaatggctta gagaggattt ctgaaaatct
     9961 ccagaacgca acaggacgca ttctaacgct gttttcatta ctcggtatag ggataggtga
    10021 tgtgtgttta atgcgtctgt gcgcttgttt tgtgccttat tttgcatttt cataatgcgg
    10081 aagagaacac ggaaaacctt aaaattaatt attggggtat ttaaggctat tttca
//
LOCUS       DAZPAC010000145         9924 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZPAC010000145 DAZPAC010000000
VERSION     DAZPAC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9924
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.145"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..611)
                     /locus_tag="ACHUPM_003048"
     CDS             complement(<1..611)
                     /locus_tag="ACHUPM_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR0870755.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHUPM_003049"
     CDS             complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHUPM_003049"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0870756.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHUPM_003050"
     CDS             complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHUPM_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR0870757.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHUPM_003051"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHUPM_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR0870758.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /locus_tag="ACHUPM_003052"
     CDS             complement(5085..6716)
                     /locus_tag="ACHUPM_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR0870759.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHUPM_003053"
     CDS             6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHUPM_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR0870760.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="ACHUPM_003054"
     CDS             7139..7411
                     /locus_tag="ACHUPM_003054"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0870761.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHUPM_003055"
     CDS             7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHUPM_003055"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR0870762.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHUPM_003056"
     CDS             7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHUPM_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFR0870763.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHUPM_003057"
     CDS             complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHUPM_003057"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR0870764.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
BASE COUNT         2517 a         2376 c         2453 g         2578 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcagga gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatgggttt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggtgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
     7741 atttctcttc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaattcct
     7801 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
     7861 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
     7921 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
     7981 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
     8041 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
     8101 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
     8161 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
     8221 gtcgcctgac tgcttcgttc attaaagtga aatttatttc tgtgggcgca tcgccgggaa
     8281 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
     8341 aatacccaga ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc
     8401 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
     8461 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
     8521 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
     8581 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
     8641 atcaaagatc tccgtgataa tacgtcccag cccccagctt ttctctaccg taattccgat
     8701 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
     8761 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
     8821 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
     8881 cagtgactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
     8941 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
     9001 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
     9061 acgcagatac atatctaaat ccagagcatt atgatgggta ataaacgggc gagcagatgc
     9121 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
     9181 gaactgacgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
     9241 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
     9301 gaatttatct ggtaaaggac gtagtgcttt agtcagcagg cgcaactcag tacagtgaat
     9361 ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
     9421 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
     9481 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
     9541 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
     9601 attcttcgca tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt
     9661 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
     9721 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
     9781 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
     9841 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
     9901 tcactaataa ataaccatca aaat
//
LOCUS       DAZPAC010000146         9913 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZPAC010000146 DAZPAC010000000
VERSION     DAZPAC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9913
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.146"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..347
                     /locus_tag="ACHUPM_003058"
     CDS             <1..347
                     /locus_tag="ACHUPM_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0870765.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
     gene            complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHUPM_003059"
     CDS             complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHUPM_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFR0870766.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1188..1808
                     /gene="fklB"
                     /locus_tag="ACHUPM_003060"
     CDS             1188..1808
                     /gene="fklB"
                     /locus_tag="ACHUPM_003060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0870767.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2117..3529
                     /gene="cycA"
                     /locus_tag="ACHUPM_003061"
                     /gene_synonym="dagA"
     CDS             2117..3529
                     /gene="cycA"
                     /locus_tag="ACHUPM_003061"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR0870768.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="ACHUPM_003062"
     CDS             complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="ACHUPM_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR0870769.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4344..5309)
                     /locus_tag="ACHUPM_003063"
     CDS             complement(4344..5309)
                     /locus_tag="ACHUPM_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0870770.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="ACHUPM_003064"
     CDS             complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="ACHUPM_003064"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0870771.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6366..6746
                     /locus_tag="ACHUPM_003065"
     CDS             6366..6746
                     /locus_tag="ACHUPM_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR0870772.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHUPM_003066"
     CDS             complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHUPM_003066"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR0870773.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHUPM_003067"
     CDS             8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHUPM_003067"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR0870774.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2304 a         2493 c         2567 g         2549 t
ORIGIN      
        1 acccttggtt tttaaaaatt gtgcatagtg aaaatcaaag taaaggtgtg cattatgcaa
       61 aatcactgcg tctcttagaa attaaccatg cgcacttgca gttaatggaa tccttattgg
      121 atgaagggaa gaaacacaat atttttaagc cagatatcga cccattgcag gtgaatatta
      181 atattgctgc gcttggggga tattatttga tcaaccagca tacgcttggc ctggtttatc
      241 acatcagtat ggtttcaccc caggcactgg aagccagacg taaggtcatc aaagagacaa
      301 tccttagctg gcttttggtt gacccttcat ctaccgcgca cgaataaaac tgccatccgg
      361 ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc ccgtcaccac
      421 gccgctggcg ttctggcgga ttttcaccat ctgcccattt tgcaaattac tcagcggctt
      481 acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc cgtggtcgcg
      541 gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt gattatcaat
      601 tccgctatct ggctggaatg gttgcgtttg ggtagtttgc ggctgttctt ctggctggcc
      661 ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt gtggtgtgac
      721 taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgcgactgaa tatctaactg
      781 cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca gaaatccgac
      841 caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca gcgggtccat
      901 gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta gttcaaagcg
      961 cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct atgaataagc
     1021 taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga tattgctatt
     1081 gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt
     1141 ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg accaccccaa
     1201 cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac
     1261 tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt attgccgatg
     1321 cgctggaagg caaacatccg gctgtgccgg ttgatgtggt gcatcgcgcg ctgcgtgaaa
     1381 tccacgaacg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg
     1441 tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc
     1501 aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg
     1561 ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt gctcgtggtg
     1621 aacccgctga attcccggtt aatggcgtga tccctggctg gattgaagca ctgactctga
     1681 tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgcg
     1741 gcgcaggcgc atccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa
     1801 tcctctaagc agcgcattct gttcccctcg aaccagaggg gagcaggcat tcagcaataa
     1861 acccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt
     1921 ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaacat aaaattaaca
     1981 ttagatctaa atcttagtat tcatcccgcg tattgttacc taatatcgat gagtcccgat
     2041 acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac acagacaggt
     2101 acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc aggctccggc
     2161 tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg ccattggcgg
     2221 tgccattggt acggggttgt ttatggggtc cggcaaaacg attagccttg ccgggccgtc
     2281 gatcattttc gtttatatga tcatcggttt tatgctcttt ttcgtgatgc gggcaatggg
     2341 ggaattgctg ctttcgaatc tggaatacaa atcttttagt gacttcgctt ccgatttact
     2401 cgggccgtgg gcaggatatt tcaccggctg gacttactgg ttctgctggg ttgtaaccgg
     2461 tatggcagac gtggtggcga tcactgctta tgctcagttc tggttccccg atctctccga
     2521 ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg ctcaatctcg ccaccgtgaa
     2581 aatgttcggt gagatggagt tctggtttgc gatgatcaaa atcgtcgcca ttgtgtcgct
     2641 gattgtcgtc ggcctggtta tggtggcgat gcactttcag tcaccgactg gtgtggaagc
     2701 gtcattcgcg catttgtgga atgatggcgg ctggttcccg aaaggtttaa gtggcttctt
     2761 tgccggattc cagatagcgg ttttcgcttt cgtagggatt gagctggtag gtacaaccgc
     2821 tgcggaaacc aaagatccgg agaaatcact gccacgcgcg attaactcca ttccgatccg
     2881 tatcattatg ttctacgtct tcgcgctgat tgtgattatg tccgtgacgc cgtggagttc
     2941 ggtagtcccg gagaagagcc cgtttgttga actgtttgtg ttggtaggtt tgcctgcggc
     3001 tgccagcgtg atcaactttg tggtgctgac ctctgcggcg tcttccgcta acagcggcgt
     3061 cttctctacc agccgtatgc tgtttggtct ggcgcaggaa ggtgtggcac cgaaagcgtt
     3121 cgctaaactt tctaagcgcg cagtacccgc gaaagggttg accttctcgt gtatctgtct
     3181 gctcggcggc gtggtgatgt tgtatgtgaa tcctagcgtg attggcgcgt tcacgatgat
     3241 tacaaccgtt tccgcgattc tgtttatgtt cgtctggaca attatccttt gctcgtacct
     3301 ggtttaccgc aaacagcgtc ctcatctgca tgagaagtcg atctacaaga tgccgctcgg
     3361 caagctgatg tgctgggtat gtatggcgtt ctttgtgttc gtggtcgtgt tgctgacact
     3421 ggaagatgac actcgccagg cgctgttggt caccccgctg tggtttatcg cactggggct
     3481 gggctggttg tttattggta agaagcgggc tgctgaactg cggaaataac cgcattatca
     3541 tgctggatgg cgcaatgcca tccagctttt agattactca cccgccagcg cgcgtgggaa
     3601 cagtacattg ttttccaggc tgatgtgatc catcaggtca tcaatcagtt cattaatacc
     3661 gttatacatc gctttccagg tggtgcaggc ttccggcggc ggtgtgacgt tattggtggt
     3721 gtgtttaatc acttccagca gttcgcccgc ttcatcgtgc tcgctttcca tcacgctgat
     3781 tggtcccatt gcctggctgc ccatgccttg tttgatcatc gggaagagga tctgctcttc
     3841 tttcatcatg tggctggata gctcttcatg cagcatagtc aggtattttg tcagcccttt
     3901 tggcacgctc ggtttgtcgg cgtgaacgcg ctcgacttta gtcgcttgca ggatcagctc
     3961 cggcagttgc tcgcggtgac gatcgtggta gcgcacgatg atatggtcaa tgatttctgc
     4021 cagcggggcg ctacgccagt ctttctcaat cggttgttca gcaagctttg ccagttcagc
     4081 ttcaatgacc tcaacatcca gttctttacg tgccgccgcg cgcgccagcg tttgcttacc
     4141 gccacagcag taatccatat catatttacg aaacagagct gaagcgcgag gaatagagag
     4201 cgccagttca cctaaaggtt ggtcgcgata agccatagct gatacctcat tcttaataat
     4261 ataagatgta ttttaaatgc atctttaagg caaaagctat aacccttacg tagcagaggc
     4321 ttatttaact cactgccagc gtgtcaactt tctgagacag attcagttaa aggtttttct
     4381 ggttttgctc tgaccgcaat gaccacgcca atcactaaca gcgcgatacc gctcagcgtc
     4441 attagcggcg gcatttgctg gcggagtaaa aaggtgtaca gcaaacctgc cagcgtttcg
     4501 aaaacaatca gcggcccgag aatcactgtc ggtaatcgct ggctggcgac gttccagcag
     4561 agtgcgccaa cccatgagca aagcacggct atcgcaacca tcagactaat aaacaccagc
     4621 ggacgggggc caaaaggtaa ggagaagtcc ggcgtttgta tattcagcca gtaacaggcg
     4681 acgagatagc cgatgagaga aaccggcagt gtgaccagcg cctgcgccgt tgcccacatc
     4741 atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt tgcgcagggc ataccacgcc
     4801 cagcagacca cggaaactaa cgccagcacg atgccagagg tataacgtgc ccagtcaaaa
     4861 tcggggagtc cgtggtttaa ctcagcaata ttcacacacg ccaggccgat gccaatacaa
     4921 accagtgccg gggcgagttt tccccatgcg agtttgccgt cgcgctggct ataaagcaga
     4981 ttggcaaaaa cgggaatcac caccggcagg gtgccgataa tcatcgtgga aacaggcgcg
     5041 ccagtacgtt gaatggcact ggcaaggcag aagtaataga taaggttgcc catcatggtg
     5101 agcatcaagg cggtaagcca gtcgcgacgc gccaactgac gcagacgcac gcgtcccagc
     5161 caggcaatgg gcagcgcaat taaccctaac gccagataac gccccatcga ctgcaacatc
     5221 gccgggtatt ccggtacgat caacggcccg acaaaaataa gcccccacat caaccctgct
     5281 aacagggcgt acagcacgcc gctaatcatt actggcatcc attggtctgt cagaagaaag
     5341 ttcaggaagc agacatgatt gctcccggca tcccgtcatt atcagagagg atgcgggagg
     5401 atgccacttt aattaactag ttattaacat taaaaagatg gcttacgctt tcggctaacg
     5461 ttgtcgttgg gcggccaatc aatttgctaa gcgttttgct gttatcaaac agaccacctt
     5521 tcgatgcgcc aacgtcagaa tccgccagca tatccgccag tccgtcgggc agtccgacgc
     5581 ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg ataggtaacc tgtttgccgc
     5641 tctgtttggt cagctctgcc gctaactgtg tcaacgtcca ggcactatcg cccgccagtt
     5701 cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg tgccgcagct gccgcataat
     5761 ctgcccgcgt tgctgaggcg attttgccat cgcccgccgc accgataaat acgccgtgtt
     5821 ccagtgctgc cggggcgctg gcgaggtagt tttcgctgta ccagccgttg cgcagcaggg
     5881 tgtaaacgat gccagaatca gccagcattt tctccgtctc aatgtgctca tcggcgaggc
     5941 cgagcgggga ggtatctgca tgtagcaggc tggtataagc gataaatttc acgccagccg
     6001 cctttgcggc attaataaca ttacgatgct gcggggcacg ttgacccact tcgctggaag
     6061 agatcagcag tagtttttcc actccctgaa gtgcagatgt cagtgcggct tcatcgccgt
     6121 agtcagcctg acgcacggta atgccttgtg ctgctagagc ctgggctttt gccggattac
     6181 gaacgatagc cactatttgg ctggcaggaa ccgttttcat caaggattca ataatatagt
     6241 gaccaagttg gccagtggca ccagtaatag cgatcatggg aagtctccat cgttttgtct
     6301 tgtgtttaaa tacgctaaca cctaaactta cttttagtaa gtacgtacaa aaaggtaagt
     6361 atgaaatgag tcaggttagc ctgtcgcaac aactgaaaga gggtaatctc tttgcggaac
     6421 agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg ttggggggtg ttgattctgg
     6481 tggcgctacg cgaaggtact catcgcttta gcgacctgcg gcgcaaaatc ggcggggtga
     6541 gcgaaaagat gcttgcgcag tcgttacagg cgttagaaca ggatggtttt cttaaccgta
     6601 tcgcgtatcc ggtggtgcca ccgcatgttg aatacagcct cacgccgctg ggcgagcagg
     6661 tgagcgaaaa ggttgctgca ctggcggact ggattgagtt gaatttgccc gaggtgttgg
     6721 cggtgcggga tgaacgtgcg gcataactgc cggatgcgcg gcctacggtg tatgacttgt
     6781 agggcggata agatgcgcca gcatcgcatc cgccaataaa tgccggatgc gattgatgat
     6841 ttataggccg aaaaagaagt gttttactta ctcaaatcca cctgataaat cgcaaacccg
     6901 atatcatcgg tcgcgacttt attcatcgga tactgccctt tctctttaat aaacgctgcg
     6961 gctttatctg acggggaagt ttcgaaacgg atatccagtt tcttatcggc agctatcggt
     7021 gctaaacgcc agttgttatc tgccgccggg tgaatttccc ccgcacgttt cgactcatca
     7081 gcaatccacg ctgccagcac cgagcggttc tcatccggtg aagcaaaagc gatatggctg
     7141 tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt tattggtggc aacgaggaac
     7201 atggcgttcg gatcaatcgg cttgccatta aaggtcaggt tcttaatcct ttccgcattg
     7261 gtattaatca tctggcactc gccgtcataa cgggcgggct gggtaacatc aatctgataa
     7321 ttcacaccat caataacatc aaagttataa gtgcggaaac catcccagtt gatgagtgac
     7381 tgtggtttcg tgctgttagg atcaatctgg ttaaactgtc cggcggagca ctccagccac
     7441 tctttcacct ctttaccgct ggctttcact acaatcagcg tattggggta gagataaaga
     7501 tcggcggcat tacggaaggt caactggcct ttttccacct ccacatagct tgccgggtca
     7561 tttttgcgac caccaacttt aaacggtgcg gcagctgaaa gcaccggcag ttttgccaga
     7621 tccggatcgc cctgaatgta atgttcgaca tacgcttttt gcgcgttgtt cacgacttgc
     7681 acggtcggat cgtcctgcac cagcgccaga tagctataca tattgtcggc agatttaccg
     7741 attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc
     7801 ttgctgtctt ccgccgcgag ggatttttta ttggcgatgt cgtaaatcgg ccgagcttcc
     7861 gctttcgcct gcgtcacctg ccatttaccg ctgtcattac tgagttgtaa gtcgaccacc
     7921 ccaagatgat cgccccacat gcccggcatt accgccggaa caccattcag cgtgcctttg
     7981 gtgatatcag ccccttcgat atcagcaaaa tctttacccg ggaaaacggc gtgagcatga
     8041 ccaaacataa tggcgttaac gcccggaatt tcactgaggt aataaactga gttttccgcc
     8101 atcactttat acggatcggc agacagcccg gaatgtgcca gaacgacaac gacatcggca
     8161 cctttctcgc gcatttcagg cacgtatttg cgcacggttt cggtaatatc attcaccgtc
     8221 actttcccgg ataaattagc tttatcccag cccatgattt gcggcggcac gacgccaata
     8281 tagccaatct tcagcgtctg tttttttccg tctttatcga ccacttcggt atctttaatt
     8341 aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa tgacgttggc atttacataa
     8401 gggaatttcg ctcccgccag cgcatttttc aggtaatcca gaccgtagtt aaattcatga
     8461 ttgccgagtg taccgaccgt ataatccagc gtattcagcg ccttataaac cggatgaaca
     8521 tcacctgttt ttaatccttt cgccgacatg taatctgcca gcggactccc ctgaatcaaa
     8581 tcgccgttat cgaccagtac gctgtttttc acttcattgc gggcatcgtt aatcaggctt
     8641 gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt tgtaataatc gaaatccatc
     8701 atgttgctat gcagatcagt ggtttccatg atacgcagat cgaccgtcgc tgcattcaca
     8761 ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact taatcatcag ggacatcctt
     8821 ttatcatcgg gaatacgaaa gaaaagggag aataaacgtc ttacttatag aacagtgaag
     8881 aatgccacaa ttttacgctt tgaaaatgat gacactatca cagttggcgc attcattaac
     8941 gatagggtat aagtaaaaca ataagttaac accgctcaca gagacgaggt ggagaaatgt
     9001 tagatcaagt atgccagctt gcacggaatg caggcgatgc cattatgcag gtctacgacg
     9061 ggacgaaacc gatggacgtc gtcagcaaag cggacaattc tccggtaacg gcagcggata
     9121 ttgccgcaca caccgttatc atggacggtt tacgtacgct gacaccggat attccggtcc
     9181 tttctgaaga agatcctccc ggttgggaag tccgtcagca ctggcagcgt tactggctgg
     9241 tagacccgct ggatggtact aaagagttta ttaaacgtaa tggcgaattc accgttaaca
     9301 ttgcgctcat tgaccatggc aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg
     9361 taatgtacag cgcggcagaa ggcaaggcct ggaaagaaga gtgcggtgtg cgcaagcaga
     9421 ttcaggtccg cgatgcgcgc ccgccgctgg tggttatcag ccgttcccat gcagatgcgg
     9481 agctgaaaga gtatctgcaa cagcttggcg aacatcagac cacgtccatc ggctcttcgc
     9541 tgaaattctg cctggtggcg gaaggacagg cgcagctgta cccgcgcttc ggaccaacga
     9601 atatttggga caccgccgct ggacatgctg tggctgcagc tgccggagcg cacgttcacg
     9661 actggcaggg taaaccgctg gattacactc cgcgcgagtc gttcctgaat ccggggttca
     9721 gagtgtctat ttactaaatt cagatggcag aaacagtgta tttcctgatt ctgccatcct
     9781 gatttctccc aacctaaaaa gttataaata aaaagagatt gtatttaaag tgcaaaaatt
     9841 caattgctaa taagttacat tttaataatg agcgtttttt gatagtttac ttctctaatg
     9901 agatatttaa tgg
//
LOCUS       DAZPAC010000147         9896 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZPAC010000147 DAZPAC010000000
VERSION     DAZPAC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.147"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(113..1108)
                     /gene="gapA"
                     /locus_tag="ACHUPM_003068"
     CDS             complement(113..1108)
                     /gene="gapA"
                     /locus_tag="ACHUPM_003068"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR0870775.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1450..1863
                     /gene="msrB"
                     /locus_tag="ACHUPM_003069"
     CDS             1450..1863
                     /gene="msrB"
                     /locus_tag="ACHUPM_003069"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR0870776.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1905..2177
                     /locus_tag="ACHUPM_003070"
     CDS             1905..2177
                     /locus_tag="ACHUPM_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR0870777.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2338..2979)
                     /gene="pncA"
                     /locus_tag="ACHUPM_003071"
     CDS             complement(2338..2979)
                     /gene="pncA"
                     /locus_tag="ACHUPM_003071"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR0870778.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2990..4006)
                     /gene="ansA"
                     /locus_tag="ACHUPM_003072"
     CDS             complement(2990..4006)
                     /gene="ansA"
                     /locus_tag="ACHUPM_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR0870779.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4173..6029)
                     /gene="sppA"
                     /locus_tag="ACHUPM_003073"
     CDS             complement(4173..6029)
                     /gene="sppA"
                     /locus_tag="ACHUPM_003073"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR0870780.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6190..6741
                     /locus_tag="ACHUPM_003074"
     CDS             6190..6741
                     /locus_tag="ACHUPM_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR0870781.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6858..7901
                     /gene="selD"
                     /locus_tag="ACHUPM_003075"
     CDS             6858..7901
                     /gene="selD"
                     /locus_tag="ACHUPM_003075"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR0870782.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7906..9867
                     /gene="topB"
                     /locus_tag="ACHUPM_003076"
     CDS             7906..9867
                     /gene="topB"
                     /locus_tag="ACHUPM_003076"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR0870783.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2454 a         2472 c         2633 g         2337 t
ORIGIN      
        1 aacaagtgag cgtttccgga ttcttacaca gccactggat cggtcaactg atccttaact
       61 gatcggcatt acgaccgaag tcgctctttt tagatcacag tttcatctca acttatttgg
      121 agatgtgagc gatcaggtcc agaactttgt tggagtaacc ggtttcgttg tcgtaccagg
      181 ataccagttt cacgaagttg tcgttcagag cgataccagc tttagcatcg aacacggaag
      241 tgcaaacttc gccgttgaaa tcggtagata ctacgtcatc ttcggtgtag cccagaacgc
      301 ctttcatttc gccttcagca gcggctttaa cggcagcttt gatctgctcg taagttgcag
      361 ctttttccag acgaacggtc aggtcaacta cggatacgtt cggggtcgga acgcggaacg
      421 ccataccagt cagtttgcca ttcagttctg gcagtacttt acctacagct ttagcagcac
      481 cggtagagga cgggatgatg ttctgggaag cgccgcggcc gccgcgccag tctttgtgag
      541 acgggccatc aacggttttc tgagtagcgg tagtagcgtg aacggtggtc atcagacctt
      601 cgatgatgcc gaagttatcg ttgataactt tagccagcgg agccaggcag ttggtggtgc
      661 aggaagcgtt ggaaacgatg tcctggccag catatttgtc gaagttagcg cctttaacga
      721 acatcggagt gttgtcttta gacggaccag tcataaccac tttcttcgca ccagcggtga
      781 tgtgtttacg agcagtttcg tcagtcagga acagaccagt tgcttcagcg acaacgtcaa
      841 caccaacttc gtcccatttc aggttagccg gatcacgttc agcggtaaca cggatttttt
      901 taccgttaac gatcagatga ccgtctttca cttcaacggt accgtcgaaa cggccgtgag
      961 tggagtcata tttcagcatg tatgccatgt aatcagcgtc taacaggtcg ttgattgcaa
     1021 cgatctcgat gtcagaacgt ttctgagcag cacggaaaac aatgcgaccg atacggccaa
     1081 aaccgttgat acctactttg atagtcatat attccaccag ctatttgtta gtgaataaaa
     1141 ggttgcctgt aaaattacaa aaaccttacg cagcgtcaag cggaatcgtg tcaatcattg
     1201 cgacaaatca atcctgtgcc taagcattac gcgactgact cgcctcactc ttcctttggg
     1261 cttgaaacca catggggtcg gcgccccgaa ttttaaaggg caattacgat aaaaatgtga
     1321 ttttcatcac gatttaggtg cagccatttc gtcgagatca agtttaggac aaaattttgc
     1381 cagttggtgt taatgttttg ttagaatcag tcacgtaatg tgagcacgat taaagtgaga
     1441 tgtgagcaaa tggctaataa accttcggca gaagaactga aaaaaaattt gtccgagatg
     1501 cagttttacg tgacgcagaa tcatgggaca gagccgccat ttacgggtcg tttactgcat
     1561 aacaagcgtg acggcgtata tcactgtttg atctgcgatg ccccgctgtt tcattcccaa
     1621 accaagtatg attccggctg tggttggccc agtttctacg aaccggtgag tgaagaatcc
     1681 attcgttata tcaaagactt gtcacatgga atgcagcgca tagaaattcg ttgcggtaac
     1741 tgtgatgccc atctggggca tgtcttccct gacgggccgc agccaacggg cgaacgttat
     1801 tgtgttaact ctgcttcttt acgcttcact gatggcgaaa acggcgaaga aatcaacggt
     1861 tgaaaaaacg attcagcaac ttattccaca ggagcggaaa aattatgaat cttgatgaca
     1921 ttatcaacag catgacgcct gaggtatacc agcgtttgtc gaccgccgtt gaactgggga
     1981 aatggcccga tggcgttgcg ttgaccgagg aacaaaaagc gaactgcctg caactggtga
     2041 tgctgtggca agctcgccat aataccgaag cacagcatat gacaattgac acaaatggtc
     2101 agatggtcat gaagagcaaa cagcagttaa aagaagattt tggtatctca gcaaagccta
     2161 ttgctatgtt taagtgaatg agttatctag tatttaagcc tgttattaag ggagggcaaa
     2221 tatacttgtt aggacgtcgt gttttagctg caagtaggtt aaatgcaaca ctgacaagta
     2281 ttatctggcc tacaatgtca ttgaggaaat gtaggccaga gatgaattca actaatatta
     2341 cccctgtgtc tcttcccagt ctgccagcgt atatagcgtt gccccagctg ctgacatctc
     2401 cataaacgcg tgcgcactgt cctggggctg gatattcacg ccacgacaac catcggtaat
     2461 cacatttacc ttataaccta actgtaacgc gtccagcacg gtaaacttca cgcaatagtc
     2521 agtagccagg cccataacga tcaattcatc gatttcatga tcgcgtaacc agtcatcgag
     2581 cgaggttttc tgccgacggc cgttatcaaa aaaggcactg taactgtcaa ctaaaggatt
     2641 ttcgccttta tggaacaccg ctgcgatcgc tttttggttc agtaacggat gtaattgtgc
     2701 gccttcactg ttctgcacac agtgatctgg ccagaaggtt tgtggcaaac cgtcgagttg
     2761 gcctggcgta taaggctcta caccgtgctg actggcaaaa ctgccgtgat tcgccgggtg
     2821 ccagtcctga ctggcgataa ccgcttcacc gcgcgactgg caccagtcaa tcaggcggtt
     2881 agcgacatcc accgtactgt caccttccgg cacggcgagc gcgccaccag cacagaaatc
     2941 attttgtaaa tcgaccagta acagggcgcg agggggcatt acagtctcct taatcatccg
     3001 gcgtcagttc gccgcgcagg ttttggctca tggccttgcg aatggtttca gtatccagtt
     3061 cctggctcag caggtaatgc agtttggtta gcgtggcttc tacagtcata tctgcaccgc
     3121 caataacgcc ggcatgggcg agggcgttac cggtggcata accacccata ttcactttac
     3181 cggacataca ttgtgtcagg ttgaccacca caataccgcg atcgctggct tcttgtaatt
     3241 cctgcaggaa ggctttgttt tgtggcgcat tacccacgcc ataggagcgc agaatcaatg
     3301 ctttcaccgg ttggcgcaga aaattgcgca cgacgtcagc agaaatccct ggataaatcg
     3361 tcactacgcc aattggttgt ggcgtgattg gatgaacgat taattcccct tcaccgtgcg
     3421 gggcgggtgg cgtattcaaa cgacgaatat ggataccagc ttccagtaac ggaggaaggt
     3481 ttggagaggc aaacgcatca aaaccatcgg catgggcttt ggtagtgcgg ttgccgcgat
     3541 acaatcggtt attgaaaaag agcgttactt cgttgatcgg ataattcgcc gcaacgtaca
     3601 acgcattcag cagattaatt tgtccgtcag agcgtaactc agccagcggg atttgtgacc
     3661 ctgtcacaat gaccggttta ccgagattct cgagcatgaa cgacagcgca gaggcagtat
     3721 acgccatcgt gtcggtgccg tgcaggatga caaaaccatc atagtcgtca tagtgcgctt
     3781 taatatcttc agcaatatgc tgccagtctt ctggcgtcat atcagaagaa tccatcagcg
     3841 gcgtatattc atgaatggtg aaatctggca tctccgggcg atggaattcc ggcatcagcg
     3901 ccagttggcg ttgtagatga cctgacaccg gaatataacc ctgctcggaa cgctgcatcc
     3961 cgatggtccc gcccgtgtag gcgacgtaaa ttgatttctt ttgcatgata ttgatatgaa
     4021 tgatatcgaa agagcaaaag tatatacgtg aggcaggagg agcgcacctg atactatgcg
     4081 gataaggaaa tatggggcga gacagcggga agaggattcg tcgacgtaaa aaataatgtg
     4141 gccgacgatc tgtcggccac tcagtacaag acttaacgca cgttggcgca ggtcaggcaa
     4201 aacgcataac ggttttgtgg gtcgttaaac gcggccagct tgtcgctttc acttttaacg
     4261 gtagaggcta ccgaggcaag cggtgcaggt aacatggcct ggaacgcatc tggcaacatt
     4321 gcccggacag aaccagacat gttgtccatc actttgtcga agaaggtcgg ttcatcaacg
     4381 tagtattcca gatgccactg tttcactttt gccagctctg ctgctttggc gaccgcatca
     4441 tcgaaatccc cgagactatc gaccagcccg ttagcttttg catcctgacc ggtccagacg
     4501 tggccctggg cgattttatc aatctgctcc ggcgtcgaat gacgcgcatc agcaaccagc
     4561 gtgataaagc gtttatagcc attctcaatg cttaattgca tcatctgctg cgcttccggc
     4621 ggcagtgccc tggtgataga aacatccgcc agcggtgaag ttgagacacc atcagtatga
     4681 acaccaatcg aatccagact attttctacg gtggtgatca cgccgaagat accgatagaa
     4741 ccggtcaggg tgctggggtt agccacaatg taattagctg gcgtggaaat ccagtaacca
     4801 ccagatgccg ccatgccgcc catcgataca accacaggct tacccgctgc ccgggctgct
     4861 gccagttcag cgcgaatcac ttcagacgcg gtaacgctgc cgcctgggct attaacacgc
     4921 aggacaatcg ctttcacttt cgggtcaagg cgagcgtcgc ggatttgtgc cgcagtggta
     4981 tcaccgccaa cattcccctg agtttcctcg ccatccataa ttgcgccatt agcgaagacg
     5041 acaccgatgc tgtcaccggt atctgccggc gttttcaatg cgtaatcgta ataactgatg
     5101 gcgcgataat ttttatcagt cttactccag ccgaactctt tggttagagc tttttcgatt
     5161 tcggcactcg atgccagtgc atcgaccagc ttgttttcca gtgcatattt cgcggtatcg
     5221 ccaccggttt tggttaaacc ctcaagcaac ccttgcgcgc cagggaatac ctgctgagca
     5281 gggatctgcc ggttagcggc aacagtattc agatagtttt gccacagctc accaatccag
     5341 cggctgtcag cttcgcgggc tgccggtgac atgtcatcac gaataaacgg ttcaacggca
     5401 gatttatacg tacccacgcg gaacacatgg gtggaaactt tcagcttatc cagcaacgat
     5461 ttgtagtaca gaccgttggt agcaaaaccg tgcaaatcca ccacgccttg cggtgacagc
     5521 caaattttat tggcgaaact ggcgagataa tattgcccct ggctgtagtt ctcgccaacg
     5581 gcataaaccg gtttcccgct gtcacgaaac tctttcaggg ctttgccgat gtactgcata
     5641 gacggctggt cgccgcctgc gaagtttttc agatccatca cgataccggt gatattgcgg
     5701 tcgtccttcg cctggcgaat ggtgttgacg atatcaaaca gtgagttttc ctgcagacga
     5761 tcggaactgg caccaagcag ctggcggctt aatttactaa accgctgaga actgtcgggt
     5821 ttatcgacga tcacaccaga aatgtccagc agcagtgcgc cacgactggc cgtttctttc
     5881 gaatcaccac cactgacctg catccagatc cccacaccaa ccagtacgag gaaaataaag
     5941 aacaggttaa gtaccatttc acggacgaaa ttcagcagac gccacgtcca tttaaaaaat
     6001 ccggcaataa atcgccaaag ggttcgcatg tattctccca acttaaggtc acacctgtca
     6061 caggcgcaat agatgtggtt atcgtaatga cacaaccggc acttgtcagc aggaattgcg
     6121 gactgtgctg taacaaaatc cactctcgtg ttaattttgt gaataaatat cacgacagga
     6181 gttaatcaaa tggatgcact cgaactattg atcaatcgcc gtagcgcctc ccgcctggct
     6241 gaacccgcgc caacgggtga acaactgcaa aacatcctgc gtgcgggtat gcgtgcgccg
     6301 gaccataagt ccatgcaacc gtggcatttt tttgtgattg aaggggaagg gcgcgagcgt
     6361 ttcagcgccg tactggaaca gggggcgatt gctgccggta gtgatgacaa agctatcgac
     6421 aaagcccgta atgcgccgtt ccgcgcaccg ctcatcatca cggtggtggc gaaatgcgaa
     6481 gaaaatcata aagtcccgcg atgggaacag gaaatgtctg ccggatgcgc ggtcatggcg
     6541 atgcaaatgg cagccgttgc ccagggattt ggcggcatct ggcgcagtgg cgcattaact
     6601 gaaagtccgg tagtgcgtga agcattcggt tgccgtgagc aggataaaat tgtcggtttt
     6661 ctctacctcg gcacgccgca gttgaaagca tctacgtcga ttaacgtccc ggacccgacg
     6721 ccgtttgtaa cttatttctg ataattgtcg cgaaactgtc tggattgtga gcaaaggcgc
     6781 aggaattcag acactctcac ttatcacttc acggaatgag ggttaccata cccggattgc
     6841 attgacagga gatgtccatg agcgagaact cgattcgttt gacccaatac agccacggag
     6901 ctggttgcgg ctgtaaaatt tccccaaaag tgttggaaac catcctgcac agtgagcagg
     6961 cgaagtttgt tgatccgaat ttgcttgtgg gtaatgagac ccgcgacgat gcggcggtgt
     7021 acgatctggg caatggcacc agcgttatca gtaccaccga cttctttatg ccgatcgttg
     7081 ataatccttt cgattttggc cgcattgcgg cgactaacgc catcagcgat atcttcgcga
     7141 tggggggcaa accgattatg gcgattgcga tcctcggctg gccgattaac aaactttccc
     7201 cggaaattgc ccgcgaagta accgaaggtg gacgctatgc atgccgtcag gcgggtattg
     7261 cgctggctgg cggtcactcc atcgatgcga cggagccgat ttttggtctg gcggtaacgg
     7321 ggatcgtacc gaccgagcgg gtgaagaaaa acagtaccgc acaagccgga tgcaaactgt
     7381 tcctgacgaa accgctgggg atcggcgttc ttaccacggc tgagaaaaaa tcactgctga
     7441 aaccagaaca tcagggacta gcgacggaag tgatgtgccg gatgaacatc gcaggcgcgt
     7501 cctttgccaa catcgaaggc gtaaaagcga tgaccgacgt tacgggtttt ggtctgctgg
     7561 gccatttgag cgaaatgtgt cagggcgcag gtgtgcaggc gcgcgttgac tatgacgcga
     7621 tcccaaaatt gccgggggtt gaagagtaca ttaagttggg cgcagtgcct ggcggcaccg
     7681 aacgtaactt tgccagctac ggtcatctga tgggtgaaat gccgcgtgaa gtgcgcgatt
     7741 tgctgtgcga tccgcaaact tctggcggtt tgctgctggc ggtcatgccg gaagcagaaa
     7801 atgaggtcaa agctacagcc gccgagtttg gcattgaact gacggcaatt ggcgaactgg
     7861 tgccagcgcg cggcggtcgt gccatggttg agattcgtta attcaatgcg gttgtttatt
     7921 gccgaaaaac cgagtctggc gcgcgccatt gctgatgtcc tgcccaaacc gcaccggaaa
     7981 ggcgatggct ttatcgagtg cggtaatggt caggtggtga cctggtgtat cggtcacctg
     8041 cttgagcagg cgcagccaga cgcctacgac agccgctatg cgcgctggaa tcttgcggat
     8101 ttgccgatta tcccggaaaa gtggcaatta cagccccgac cctccgtgac caaacaactt
     8161 aacgtcatca aacgcttcct gcatgaagcc agcgaaatcg ttcacgccgg ggacccggat
     8221 cgtgaagggc aactgctggt ggatgaagtg ctggactatc tgcaactggc accggaaaag
     8281 cgccagcagg tgcagcgttg cttgataaac gacctgaacc cgcaggcggt tgagcgggcg
     8341 ctcgaccgtc ttcggtccaa cagtgagttt gtgccattgt gcgtttctgc gctggcgcga
     8401 gcgcgtgccg actggctgta cggcatcaat atgacccgtg cgtataccat tctcggtcgc
     8461 aatgccggtt atcagggcgt actttccgtg ggacgcgtgc agacacccgt gctcgggctg
     8521 gtggtgcgcc gcgatgaaga gattgaaaac ttcgtggcga aagacttctt tgaagtcaaa
     8581 gcacatatcg tgacacctgc cgatgagcgg tttaccgcta tctggcaacc gagcgaagcg
     8641 tgtgaaccgt accaggatga agaagggcgc ttgttacatc gtccactggc ggagcatgtg
     8701 gttaaccgca ttagtggtca accagcaatt gtcaccagct ataacgataa acgggaatca
     8761 gaatccgcgc cgctgccttt ttcgctttcg gcgttgcaga ttgaagcggc aaaacgcttt
     8821 ggtctgagcg cgcagaacgt gcttgatatc tgccagaagc tgtacgaaac gcacaagcta
     8881 atcacttatc cgcgttccga ttgtcgctat ttgccagaag aacattttgc cggacgccac
     8941 gcggtgatga atgccatcag cgttcatgca ccagatctgt tgctgcagcc agtggtagat
     9001 ccagatatac gcaaccgctg ttgggatgac aaaaaggtcg atgcacacca cgccatcatc
     9061 ccgaccgcac ggagttcagc catcaacctg acggagaacg aagcgaaggt ctataacctg
     9121 attgcccgtc agtacttgat gcagttctgc ccggatgtgg tgttccgcaa gtgtgttatc
     9181 gaactggaca ttgccaaagg caaatttgtc gctaaagcgc gttttcttgc tgaagcaggc
     9241 tggcgcacgc tgttaggcag caaagagcgc gatgaagaaa acgacggtac gccattgcct
     9301 gtggtggcga aaggcgatga gttgctgtgt gaaaaaggtg aagtggtaga gcggcaaacc
     9361 cagccgccgc gccattttac cgatgcaaca ctgctttcgg cgatgaccgg gatcgcgcgc
     9421 tttgtgcagg acaaagatct gaaaaagatc cttcgtgcga ccgatggtct ggggacagaa
     9481 gcaacccgtg ccgggattat tgaactgctg ttcaagcgtg gttttctgac caaaaaaggg
     9541 cgctatatcc actctaccga cgccggaaaa gcgttattcc attcgctgcc agaaatggcg
     9601 acgcgaccgg acatgaccgc gcactgggaa tcggtgctga cgcaaatcag cgaaaagcag
     9661 tgtcgctatc aggactttat gcagccgctg gtggggacgc tatatcagct tattgatcaa
     9721 gccaaacgta cgccggtgcg gcagtttcgc ggcattgtgg ctccgggcag tggtggcagt
     9781 gctgataaga aaaaggctgc accgcgtaaa cgtagtgcga aaaaaagtcc gccagcagat
     9841 gaagtcggaa gcggggcgat agcgtaagcg agtgaatctt tcgtgctatt cgagtc
//
LOCUS       DAZPAC010000148         9829 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZPAC010000148 DAZPAC010000000
VERSION     DAZPAC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9829
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.148"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..190
                     /gene="bdm"
                     /locus_tag="ACHUPM_003077"
     CDS             <1..190
                     /gene="bdm"
                     /locus_tag="ACHUPM_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFR0870784.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            292..429
                     /gene="sra"
                     /locus_tag="ACHUPM_003078"
     CDS             292..429
                     /gene="sra"
                     /locus_tag="ACHUPM_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFR0870785.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            586..2283
                     /gene="maeA"
                     /locus_tag="ACHUPM_003079"
     CDS             586..2283
                     /gene="maeA"
                     /locus_tag="ACHUPM_003079"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0870786.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDIGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2417..3419
                     /gene="adhP"
                     /locus_tag="ACHUPM_003080"
                     /pseudo
     CDS             2417..3419
                     /gene="adhP"
                     /locus_tag="ACHUPM_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3565..3849
                     /locus_tag="ACHUPM_003081"
     CDS             3565..3849
                     /locus_tag="ACHUPM_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0870787.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4077..4730)
                     /gene="fdnI"
                     /locus_tag="ACHUPM_003082"
     CDS             complement(4077..4730)
                     /gene="fdnI"
                     /locus_tag="ACHUPM_003082"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR0870788.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4723..5607)
                     /gene="fdnH"
                     /locus_tag="ACHUPM_003083"
     CDS             complement(4723..5607)
                     /gene="fdnH"
                     /locus_tag="ACHUPM_003083"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR0870789.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5620..8667)
                     /gene="fdnG"
                     /locus_tag="ACHUPM_003084"
     CDS             complement(5620..8667)
                     /gene="fdnG"
                     /locus_tag="ACHUPM_003084"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8080..8082),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0870790.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8899..9780
                     /gene="yddG"
                     /locus_tag="ACHUPM_003085"
     CDS             8899..9780
                     /gene="yddG"
                     /locus_tag="ACHUPM_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR0870791.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2368 a         2585 c         2502 g         2374 t
ORIGIN      
        1 ttcaacagca gaacccgctc tggttaatgc cattgagcaa ggccttcggg cagagcaagg
       61 tgttgtcact gaagatgaca ttctcatgga gctgaccaaa tgggtggaag cttctgataa
      121 cgacatcctc agtgatatct accagcaaac gatcaattat gtggtcagtg gccagcaccc
      181 tacgctttaa ggtgctatgc ttgatcggca acctaattta ggggtttagc acgtgtttct
      241 tcgctacggc gatgttgtcc ttaaaactag caacaggatt gaggagttaa aatgaaatcg
      301 aaccgtcagg cacgtcatat tcttggactg gaccataaaa tttctaacca gcgcaaaata
      361 gttaccgaag gtgacaaatc cagcgtggta aataacccaa ccggcagaaa acgccccgct
      421 gaaaagtaat tcataaccat cagtcctcaa tgacgattaa acaccattgc ctgcgcaatg
      481 gtgtttttgt ttttatctgc tttatacttg gggccgacgc cctggcggta aagcaaagac
      541 gataaaagcg tgccagggat ggatattcaa aaaagagtga gtgacatgga accaaaaaca
      601 aaaaaacagc gttcgcttta tatcccttac gctggccctg tactgctgga atttccgttg
      661 ttgaataaag gcagtgcctt cagcatggaa gaacgccgta acttcaacct gctggggtta
      721 ctgccggaag tggtcgaaac catcgaagaa caagcggaac gagcatggat ccagtatcag
      781 ggattcaaaa ccgaaatcga caaacacatc tacctgcgta acatccagga caccaacgaa
      841 accctcttct accgtctggt aaacaatcat cttgatgaga tgatgcctgt tatttatacc
      901 ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct accgccgttc acgcggcgtg
      961 tttatctctt accagaaccg ccacaatatg gacgatattc tgcaaaacgt accgaaccat
     1021 aatatcaaag tgattgtggt gactgacggt gaacgtattc tggggcttgg tgaccagggc
     1081 atcggcggga tgggcattcc gatcggtaaa ctgtcgctct ataccgcctg tggcggcatc
     1141 agcccggcgt atacccttcc ggtggtgctg gatatcggaa cgaacaacca acaactgctt
     1201 aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca ctgacgacga atactatgaa
     1261 ttcgttgatg aatttatcca ggctgtgaaa caacgctggc cagacgtgct gttgcagttt
     1321 gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc gctatcgcaa tgaaatttgt
     1381 tcttttaacg atgacattca gggcactgcg gcggtaacag tcggcacact gatcgcagca
     1441 agccgagcgg caggtggtca gttaagcgag aaaaaaatcg tcttccttgg cgcaggttca
     1501 gcgggatgcg gcattgccga aatgatcatc gcccagaccc agcgcgaagg attaagcgag
     1561 gaagcggcgc ggcagaaagt ctttatggtc gatcgctttg gcctgctgac ggacaagatg
     1621 ccgaacctgc tgcctttcca gaccaaactg gtgcagaaac gcgaaaacct cagtgactgg
     1681 gataccgaca gcgatgtgct gtcactgctg gatgtggtgc gcaatgtaaa accagatatt
     1741 ctgattggcg tctcaggaca gaccgggctg tttacggaag agatcatccg tgagatgcat
     1801 aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc cgacgtcacg cgtggaagcc
     1861 acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc tggtcgccac gggcagcccg
     1921 tttaatccag tggtatggaa agataaaatc taccctatcg cccagtgtaa caacgccttt
     1981 attttcccgg gcatcggcct gggggttatt gcttccggcg cgtcacgtat caccgatgag
     2041 atgctgatgt cggcaagtga aacgcttgca cagtattcgc cgctggtgct gaacggcgaa
     2101 ggtctggtac taccggaact aaaagatatt cagaaagtct cccgtgcaat tgcgtttgcg
     2161 gttggcaaaa tggcgcagca gcaaggcgtg gcggtgaaaa cctctgccga agctttgcaa
     2221 caagccattg acgataattt ctggcaagcc gaataccgcg actaccgccg tacctccatc
     2281 taagcctgcg cccggtagtg aaggctaccg ggcaattttc ctctcccttt ttcagatctc
     2341 atccatactg ggtagtggcg aataaatctc atttgcctca cctgctatgc agaacatcat
     2401 ccgaaaagga ggaactatga aggctgcagt tgttacgaag gatcatcatg ttgacgttac
     2461 ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg ctgaaaatgg agtgttgtgg
     2521 tgtatgtcat accgatcttc atgttaagaa tggcgatttt ggtgacaaaa ccgacgtaat
     2581 tctgggccat gaaggcatcg gtgtggtggc agaagtgggt ccaggtgtca cctcattaaa
     2641 accaggcgat cgtgccagcg tggcgtggtt ctacgaagga tgcggtcatt gcgaatactg
     2701 taacagtggt aacgaaacgc tctgccgttc agttaaaaat gccggataca gcgttgatgg
     2761 cggaagagtg catcgtggtc gccgattacg cggtaaaagt gccagatggt ctggactcgg
     2821 cggcggccag cagcattacc tgtgcggggg tcaccaccta caaagccgtt aagctgtcaa
     2881 aaattcgtcc cgggcagtgg attgctatct acggtcttgg cggtctgggt aacctcgccc
     2941 tgcaatacgc aaagaatgtc tttaacgcca aagtgatcgc cattgatgtc aatgatgagc
     3001 agttaaaact ggcaaccgaa atgggtgcag atttagcgat taactcacgc accgaagacg
     3061 ccgccaaaat tgtgcaggag aaaaccggtg gcgctcactc tgcggtggta acagcagtag
     3121 ctaaagctgc gtttaactcg gcagttgatg ctgtccgtgc aggcggtcgt gttgtggctg
     3181 tcggtctgcc gccggagtct atgagcctgg atatcccacg tcttgtgctg gatggcattg
     3241 aagtggtcgg ttcgctggtc ggcacgcgcc aggatctaac cgaagccttc cagtttgccg
     3301 ccgaaggcaa agtggtgccg aaagtcgccc tgcgtccgtt agcggacatc aacaccatct
     3361 ttaccgagat ggaagaaggc aaaatccgtg gccgtatggt gattgatttc cgtcactaag
     3421 aggtctttgc tgcgactccc atgttcgggt cgcagcatcg cacactctcc aacatgaaat
     3481 ggctaaatgg attattgttg cgtaggaagg aacaataatg atagtgttcc cagagaagga
     3541 acgctactga gagtaaagac actaatgaaa atggccaatc atccccgccc gggggacatt
     3601 attcaggaat cactggacga acttaatgtc agcctgcgcg agtttgccag agcaatggaa
     3661 attgcgccct caacggcaag ccgattgctg accggaaaag cagctttgac gccagaaatg
     3721 gcaattaaac tttccgtggt gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac
     3781 gcctggagtc tggcagaggc agaaaaaacg gtggatgtgt cgcgattacg ccgtctggtt
     3841 acgcaataaa aaatggcacc ttataaagcg ccatttgaaa ctgatgacaa acgcaacatt
     3901 gcctgatgcg ctacgcttat caggcctaca cgttcacttc aatctattgt atttgaaggg
     3961 ttttcatgcc ggataaggca ttcacgacgc atccgggatg aacaaagccc acgttgtcta
     4021 caatatgaaa atggcgcctt ttacagcgcc attttcagct taaagttcaa aagagattat
     4081 atcccttctt cactctcttt tttcgcttct gccttctcga tttcacgata ccagcgcgga
     4141 tggtgtttct tcgcccagcg acggctcacc ttcccttcga tcatcccttt aatcgaccct
     4201 ttcacccaga atgccatata catatggatc aggatggcgt ggatcaggat gatacccgca
     4261 gccgcgtgga tcagcaggct gtagcgaaca acctgcatcg ggaagtactg cgcaaagtac
     4321 ggacgccaga taatcacccc ggtcaccagc agcacgaaaa tcatgctcat gatcgaccag
     4381 aacatcatct tttgcccggc gttgtactta ccaacatccg ccactttgtg ctcattgcct
     4441 tttaatactt cgacaatgtt caacagccac ggaatatctt tcttatccgg gatgttgtga
     4501 tgcacaaaac gcacaaacat aaacatcagt gcgacgaaaa tcgcaatgcc gaagaacggg
     4561 tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg tcagccattg cagcgtcggg
     4621 aagaagaacg aaatcccgga cagcgccacc aggaagaagc aaatcaccac ggtccagtga
     4681 caggcgcgat caataaattt ggtgcgcaca atcattttcg acttactcat gatgatcctc
     4741 ctcgtcatcg tccacttcct tattcgggcc aataccgatg tagtggaaaa tcaacccggc
     4801 aaaagtggca ataaagccag ccgctgccag cggtttcaac gcgcctttcc acaggcttac
     4861 cgaggtgtcg atcttcggat ctttcggcag accgtaatac agctccggct gatcggcgtg
     4921 atgcagcacg tacataacgt gcgtaccacc gacccctttc gggttgtaga cgccagcatg
     4981 ttcgtaacca cgcgctttca gtttcgccac gcgctcttcc gccagctcca gcatctcctt
     5041 cttggtgccg aagtggatag ccccggtcgg acaggttttc acacaagccg gttcctggcc
     5101 gacgctgacg cgatcgacgc agagcgtgca tttatatacc cggttatcct ctttgttgag
     5161 gcgcggaata ttaaacggac acccggcaat gcagtaacca cagccgatgc agttttccga
     5221 ctggaaatcg acaatcccgt tagcgtactg aatgattgca ccagcagacg ggcacgcctt
     5281 caggcagccg ggatcttcac agtgcataca gccgtcttta cggatcagcc actccagctt
     5341 gccgttctgt tcggtttcgc taaagcgcat caccgtccag gacttggcgc tcagatcggc
     5401 ggggttatcg taaaccccga cgcagtgccc cacttcatca cggatgtcgt tccactccga
     5461 acacgccacc tgacaggctt tacagccgat acaggtggaa acgtcgataa gttttgcgac
     5521 ttctgctttg taatcacgca cctgagaagg cggcgtgatg gagttagttg cggacctttt
     5581 gataatatcc tgcgtttcca tagccatctg ttcgccccct tacgccttct cgatgttgac
     5641 taagaacgct ttatattccg gcgtttgcga gtttgcatca ccgacattcg gcgtcagagt
     5701 gttagcgata taacctttac gcgcgacacc ctcaaagccc cagtggattg gaatacccac
     5761 cgtttcaacc tgctgaccgt ttacattcag cggtttcaga cggcgcgtta ccaccgccac
     5821 cgcgcggata aagccacgct tgcttgagac agtgacacga tcgccattat taatgccttt
     5881 cgccgccgcc agcgtttcgc tgatttccac aaactgttcc ggctgagcaa ttgcgttgag
     5941 caatgcgtgc ttggtccagg tgtggaagtg ctcggtcaga cgataggtcg tacccacata
     6001 cgggaactgc tcttttttac ccatccgcag cgcgtcttgt tcatacagac gaacaaccgg
     6061 gttagacacc acgttcggat gcagcgggtt agtgcccagc ggcgtttcaa tcggctcgta
     6121 gtgttccggg aacggacctt ccgccatttt gttgatggca aacaggcgtc ccatcccttc
     6181 cggctgcatg ataaacggcc cggttggcgt acccggtgcg gcattgccga agtccggaat
     6241 atcgttaccc gtccacttgc tgccgttcca ctggatcagc atccgtttcg gatcccacgg
     6301 tttaccattg atatccgccg aagcacggtt gtagagcacg cgacggttga gcggccacgc
     6361 ccaggcccat cccagcgtat tccccagacc ggatgggtcg gagttatcgc ggttagccat
     6421 ctggttgccc tgctctgtcc agctaccggt gtagatccag caagaagatg cggttgtacc
     6481 gtcatcacgc agatgcgcaa agctactcag caactgacct ttcttcgcaa tcagcacgcc
     6541 attagcgtca tagagatctt ccagcgcata gccgttattc tctttcgcca cttcgtcaga
     6601 ttgcggttcg tgcggctgct tgtagttcca gctcatcttc atcagcggtt ctacgccttt
     6661 accaccttcg gcctggtaca gctcgcgcag atgatggtag atacccgcca gaatttcgcc
     6721 gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag tgccactgca accagcgacc
     6781 ggagttagcg atagaaccat cttcttcggc aaagcaggtc gaaggcagac ggaatacttc
     6841 agtctgaata gacgccggat cgacatcgtt cgactcgccg tggttctgcc agaaggtaga
     6901 ggtttcagtc accagcggat cgataaccac catgtacttc agcttgctca ggcagctcac
     6961 cactttgttt ttgtccggga aggacgcaac cgggttaaag ccctggcaga agtaaccggt
     7021 gactttgcct tcgtccatca tgttgaaata cttgatgacg tcgtaggtct ggtcccactt
     7081 cggcagccag tcatagcccc agttgttctc tttctgcgcg gcatcgccat agaaagattt
     7141 catcaggcta acgaagaact tcggatagtt gctccagtag ttcacctgat cagccagcgt
     7201 cgctttcggc gtgttcgctt ccagatacga ctgcaaatca acctgttttt ctgacggaag
     7261 cgtcagataa cctggcaggc tggtagagag cagacctaag tcagtcagcc cctgaatgtt
     7321 ggagtgacca cgcaatgcgt tcacgccgcc accggccata cccatgttac cgagcagcaa
     7381 ctggatcatc gccatagtac ggatgttctg cgcacccacg gtgtgctgcg tccagcccag
     7441 cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg gaggccagca cttcacacac
     7501 tttcaggaag tcggcttttg gcgtaccgca gatgttttca acgacgtccg gcgtgtagcg
     7561 ggaaacgtgc gctttcagca ggttccacac acagcgcgga tgagtcagtg tttcatcgcg
     7621 ttttgcatag ccgttttcat cgaactgata gttccaggac gacttatcat actggcgttt
     7681 ttcagcgtcg tagccgctga acaggccgtc ttcgaaagca aaatcatcac gcaccagcag
     7741 gctggcgttg gtgtaatgtt taacgtattc ggcgttgatt ttgttgtttt cgatcaggta
     7801 gcgcaaaacg ccagacagga acgtaatgtc cgtaccggaa cgaataggtg cgtaaatatc
     7861 cgccacagaa gcggtacgcg taaaacgagg atcgacaacg atcaaggttg catcgttgtt
     7921 gtttttcgct tccatcgccc agcggaaacc gacgggatgc gcttcagcag cgttaccgcc
     7981 cattaccatt acgacgttag cgtttttgat atccacccag tggttggtca tcgcaccgcg
     8041 accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt cagacgcgcg cctggttgtc
     8101 taccgccagc atcccgaggg agcgggcaaa tttctgggtc agcatcccgg tttcgttgct
     8161 ggcaccggag gcacacagca taccggtaga aagccaacgg tttaccgtta cgccctgctc
     8221 gttcttttca ataaagttag cgtcacggtc agctttcatc agcttcgcaa tacgggagaa
     8281 tgcttcttcc cagctaatgc gctgccattt gtcagaacct ggcgcacgat attccgggta
     8341 gcgcagacgg ttttcgctgt tgacgtaatc cagcaaaccg gccccttttg ggcacagcgc
     8401 accacggctt accggatgat ccgggtcacc ttcaatgtga taaatcgctt ctctggcgtt
     8461 tttcgcgcca tcacccaggc tatacatcaa tagcccgcaa cctacggaac agtatgtgca
     8521 ggtgttacgg atctctttag cgcgtaataa tttgtagttt cgcgcctgag ccagtgcttg
     8581 cttcggggca aagcccaatg ccgctaccgt tgttccagcc ataccgcccg cgcagatttt
     8641 aaaaaattgt ctgcgactga cgtccattgc tttcctcttt tttcaggggt attactgcgc
     8701 gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa atcaaaaaga acacgtgtat
     8761 gccacaaaat aatccgcctg cgcaaattaa cctacctcaa tagcggtaga aaaacgcacc
     8821 actgcctgac aggccagtta aaaaaatgct ataaaattca gcttaatttt taacggcaag
     8881 agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct aatagcgatc
     8941 gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct cggtccggtc
     9001 ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac ggttggattt
     9061 ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt attcgtcagc
     9121 tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca ggcgattgaa
     9181 gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat tctgtttaat
     9241 ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt cggcgtctgc
     9301 tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa tatcaccacc
     9361 agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc ctattgcaca
     9421 gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct gctaacggga
     9481 gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt tagcacgccc
     9541 gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatgc tgcatggaat
     9601 gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt tacgcctgta
     9661 ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc gttctggcaa
     9721 ggcgcgctaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg tcgtggttaa
     9781 aataattgcc gggtctacgg cccggcagtt aatcagataa attccgaca
//
LOCUS       DAZPAC010000149         9762 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZPAC010000149 DAZPAC010000000
VERSION     DAZPAC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.149"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..151)
                     /locus_tag="ACHUPM_003086"
     CDS             complement(<1..151)
                     /locus_tag="ACHUPM_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870792.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLN"
     gene            229..1323
                     /gene="ddlA"
                     /locus_tag="ACHUPM_003087"
     CDS             229..1323
                     /gene="ddlA"
                     /locus_tag="ACHUPM_003087"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0870793.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(1347..1559)
                     /locus_tag="ACHUPM_003088"
     CDS             complement(1347..1559)
                     /locus_tag="ACHUPM_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR0870794.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1819..2127
                     /locus_tag="ACHUPM_003089"
     CDS             1819..2127
                     /locus_tag="ACHUPM_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR0870795.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2186..3280)
                     /gene="yaiW"
                     /locus_tag="ACHUPM_003090"
     CDS             complement(2186..3280)
                     /gene="yaiW"
                     /locus_tag="ACHUPM_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR0870796.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3293..4513)
                     /gene="sbmA"
                     /locus_tag="ACHUPM_003091"
     CDS             complement(3293..4513)
                     /gene="sbmA"
                     /locus_tag="ACHUPM_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR0870797.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4865..6022
                     /gene="ampH"
                     /locus_tag="ACHUPM_003092"
     CDS             4865..6022
                     /gene="ampH"
                     /locus_tag="ACHUPM_003092"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR0870798.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6023..6646)
                     /gene="iprA"
                     /locus_tag="ACHUPM_003093"
     CDS             complement(6023..6646)
                     /gene="iprA"
                     /locus_tag="ACHUPM_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFR0870799.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6734..9616)
                     /locus_tag="ACHUPM_003094"
     CDS             complement(6734..9616)
                     /locus_tag="ACHUPM_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0870800.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITNGGVLDVNTNMNDISSVFAT
                     DTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMNSD
                     GHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYIDD
                     TKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQ
                     DTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGNNA
                     KWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSA
                     TMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNDMK
                     VDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTE
                     GSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2510 a         2414 c         2349 g         2489 t
ORIGIN      
        1 aattaagaga aaaaaactca ccgcattgcc atttaagcca ggaataaatt agcttcccgt
       61 tcatcatttt tgtgatctcg cgcacatctt tgagataacg cactttcttc accagtacgt
      121 tgcacgcttt gtcagcagaa gattgcaaca atctttcaga aggccgcgaa tcccccgttt
      181 tgtcgtggta atgtatgcac tttgtgttag acgggatagg tttttaagat ggaaaaactg
      241 cgggtaggaa tcgtttttgg tggtaaatca gcggaacatg aagtgtctct gcaatcggca
      301 aaaaacattg tcgatgccat tgataaaagt cgcttcgacg ttgtgctgct gggcattgat
      361 aaacaagggc aatggcacgt cagcgatgcc agcaattatc tgctaaatgc agacgatcct
      421 gcccatattg cgttgcgccc ttcggcgacc agccttgcgc aggtgccagg taaacatgag
      481 catcagctta tcgacgcgca aaacggtcag ccgttgccga cggtggatgt cattttcccg
      541 attgtccacg gtacgctggg cgaagatggt tccttgcagg gaatgctgcg ggtcgccaat
      601 ttaccgtttg taggttctga tgttctggct tcagcagcct gtatggataa agatgtcacc
      661 aaacgtctgc tacgcgatgc cgggctgaac attgcgccat ttattaccct gacgcgcgct
      721 aatcgtcaca acatcagttt tgccgaagtg gagtctaaac tggggttacc actgtttgta
      781 aaaccggcta atcagggctc ttctgttggt gtcagcaaag taaccagtga agaacagtac
      841 gcaattgccg tcgatctggc gttcgagttc gatcataaag tgatcgttga gcaagggatc
      901 aaaggtcgtg agatcgaatg cgcagttctg ggcaacgaca atccgcaagc cagcacctgt
      961 ggcgagatcg tactcaccag cgatttctat gcctacgaca ccaagtacat tgacgaagat
     1021 ggcgcgaaag tggtagttcc ggcagccatt gcaccagaga tcaacgataa gatccgggcg
     1081 attgccgttc aggcttatca gacgttggga tgcgcaggca tggcgcgtgt agacgtgttt
     1141 ttaaccccag agaacgaagt ggtgatcaac gagatcaaca cactgcctgg cttcaccaac
     1201 atcagtatgt atccgaagct gtggcaagcc agcggtctgg gttacaccga tctgatcaca
     1261 cgtttgattg agctggcgct gaagcgtcac gctgcggata acgcattgaa agccacaatg
     1321 taatatttta acgcccgata atattcttat tgggcgtttt cagtttcttc atcgcgacga
     1381 cgaataatca accccgccag ccagaaacta atcacccacg tcaccagacc gacggcatag
     1441 gtctgccaac cttttgcttc aaatcccagt aaccccacca cgccattaag aatgaatata
     1501 aggccaatgg cgaacgcata gtagtgccag tcacggcgga tttttacagg cagattcatg
     1561 gcagctccgt taacgaaaaa caacatcctt gcatattctg taacaaccgt ctgtggtgta
     1621 gacaacattt tcgtaattgt taataaattt aacttaaggt tgccattttg taactcacaa
     1681 cgaagattca cgcttcggga atattcccaa catcaattta ccatgattct gatattgaca
     1741 atctaaatga cctgtcagac ttgccataag ccgctggtat cacaccagaa gagaagcaga
     1801 tgacttccgg aggtttttat ggctgatttc accctgtcaa aatcgctgtt tagcggaaaa
     1861 tatcgcaatg cctcttcaac gcctggcaac attgcctatg cgttgtttgt gctgttttgc
     1921 ttttgggctg gggcgcaatt gctgaacctg ttagtgcatg cgcccggcgt ctatgagcgt
     1981 ttaatgcagg tccaggaaac aggtcgccca cgggtggaaa ttggtttagg tgtcggcacc
     2041 attttcgggc tgatcccgtt tttagtaggc tgcctcattt ttgcagtggt ggcgctatgg
     2101 ctgcactggc gacatcgccg ccagtaagat ttacgttgcc agatacggcc taaaacgcct
     2161 tatctggcat gacattcctg tttgatcact gtttcataca acgcgcccgc cgttcgtcta
     2221 cgcgcttcgc aaaccaggcc gtagtcaggt tgcgggtgat tttcgggctt tccagttgaa
     2281 tgccaggcaa catttcgcgg ggtagtgatt tacccgtttt cgcttcggca agttgataaa
     2341 ctttcttgta cagcgccgtc tcctcaaaag agaagctgtc acctttctct aactgacggc
     2401 gaatttcgct gtcgctcatc cccagttttc ccgccagttt gcgcgttgcc agttcggttt
     2461 tcccgggttc tttactgtcg taacgaatta aatcaccgtc cagtgccagc ttcacgccgc
     2521 tggccttact gactgcgttc tgaaacgcgg cattacggct ggcgtaccag ccagcgttaa
     2581 aatcagcaaa gcggtatatc ggtgcgctat agctggcggg ataattcagt aaatggtaag
     2641 taccaaacca caacccgccg cggcgactga agacttcctg acgaactgta ccgtccattt
     2701 tccacggata ccctttggta tgctgttcgg caaaagcaat gctgacctgc atcggcccac
     2761 cggtgcgcac cggattaagc gaaccaaaca gcgtctgccc cattggtacc atgctgatca
     2821 gatcgtcaaa aatcgcgctc aattgctttt ctgtacgcac cgaatctaac cgttcgctat
     2881 aacttttgcc gtttggcgat ttgattttta gcgcggtatg aaccagaaaa gcaggaatgt
     2941 gcatccgttc agcacgacgg tcaatctctt gccaggcgat cttgcttaag cccggaacgg
     3001 ccggatctgc ctgataattt gactcctgct gcgccaccgc cagcaccgag cagacgtttt
     3061 ccagcgtcgg tgccagcccc tgactttcaa aggtgatggc gagatctttc gcccaggcat
     3121 cgcggtcttt cacgctcgca ggcatcttct gccgcacgac gctcgctaca tctatcgctt
     3181 tttcgccctt tttcaacggt tgcggagcct gactactaca ccccgctaaa accagaaccg
     3241 ccaacaatga cagcgaagat agatgattga cgcgcgacat tacttctcct ttttagctca
     3301 aggtatgggt tacttcctga attttgtcac catccagctc atgttcaaag ctgcgcagac
     3361 gtttgtagat agacatcaac tcaaccagtg tggtccatga gttaatcagg tactggaaag
     3421 caccgcgaac ctgaccaaaa acgttggtaa tctgcgtcat caggccgagc gtaatcgtac
     3481 cggcaacaat tgacggaaac agcaagaaca aaccgaaaac gttatcgacc tgcaaataga
     3541 gaatgcgggc gatgttgaaa tacatatagt gaaaatagag gcgaaaatag tttttccgta
     3601 cggcgctaaa cagctcgcgt accgtaggcg gcgtcgcgcg cgtggcatcg tcttcaccat
     3661 aaaccagctc tttacggtag gcggcctcta ctcgctggtt tttaaactcc agccccggca
     3721 gtttgatccc taccactgcc agccatccgg tccccatcag cgaccagacg atagcggcaa
     3781 tcaccagacc atacggaatg tgcccgataa tcggcagctc cggcacatgc gcggagagcg
     3841 ttaccagcac cggcaggaag gcgatcaacg tcatgatggc gttgataaaa ctgacgccca
     3901 tattctccag cgttgaagca aaacgcatgg tgtcttcctg cacacgctgt gcggcacctt
     3961 cgatatgacg cagttgttgc cagttcgcca tgtaatattc gttcatcgcc gtacgccagc
     4021 ggaacacata gtgactgaca aagaagttgt tcagtacact gatcaccacc gcgatcagcg
     4081 caatccccag aaagacgccc acttcgcggt aaaattgttc gatagtgact ttatgcggcg
     4141 aacttagcgc ggtttgaatc agatcataga acggtgcata ccaggcgttg acggcgaccc
     4201 cgacttccac caaaaaccag gtgacgaaga tgatcagtgc agtaccgagt attgaccagt
     4261 attgccaacg gtgcgggctg tagataaacc agaaaaatgc aaaaagtcct acgcaaacaa
     4321 tgtagtaagc gtaaaaaatc aggaaatcca acgaccagaa acgcgcggcg ctaatcggga
     4381 tctgcccgga agcgccggtg atacgcgcca cccagtctcc cccaccggct tgccagaaga
     4441 taacggcaat caatgcccaa acaaaggccg agagaaaaaa cgctcccggc tttgggaaaa
     4501 aagacttaaa catatgcact cctgctaact tcttatcgtt ttaacaactg ctgtgtatta
     4561 accgcatgac cactacaaag cgtcagccaa atgaaaccgc ccattgtgac ccatagcaag
     4621 gcgcttagtt cgccagcctg accataagaa ttgtttcgac ggatttcaca aaccaaagat
     4681 tgtttacata agcggattaa cacgcgcagg atcacaaatg caggcattgg tgagcaggaa
     4741 aaagatcgtg tttacgcgcc gttacgtttt taacactatt attgcactaa ttcgcccttt
     4801 gcaatccatc aatgagtagt ataaatacgc tcagttacct tcattcaatc tatggacacc
     4861 accgttgaaa cgtagtctgc ttttttctgc cgtgctgtgt gcggcgtcat tgacctccgt
     4921 ccatgcggcg cagccgatca ctgaaccgga gtttgcctct gatattgtcg atcgttatgc
     4981 cgatcatatt ttttacggca gcggtgcaac ggggatggcg ctggttgtta tcgacggtaa
     5041 ccagcgcgtc tttcgtagtt atggcgaaac gcgacctggt aataacgttc gcccgcagct
     5101 ggattcggtc gtgcgtattg cttccctcac caaactaatg accagtgaaa tgctggtgaa
     5161 attgctcgac caggggaccg taaagttaaa cgatccgcta agtaaatatg ctccgccagg
     5221 cgcacgcgtg ccgacctaca atgggacgcc cattacccta gtgaatctgg caacccatac
     5281 cagcgccctg ccccgtgaac agcccggtgg cgcggcacat cgtccggtgt ttgtctggcc
     5341 aacgcgcgag caacgctgga aatacctttc tacggcgaag ctgaaagccg cgccaggcag
     5401 tcaggcggcg tactctaacc ttgcgttcga cctgctggca gatgcactgg cgaatgcgtc
     5461 cggcaagcct tatacccagt tgtttgaaga gcagattacc cgtccattgg ggatgaaaga
     5521 caccacctac accccctcac cggatcagtg ccgccgttta atggttgctg agcgcggtgc
     5581 cagtccgtgc aataacacgc tggcggcaat tggcagcggc ggcgtctatt ccacgcctgg
     5641 cgatatgatg cgctggatgc agcagtattt gtcatctgat ttttatcagc gcagtaacca
     5701 ggcagatcgc atgcagacgt tgatttatca gcgcgcgcag tttacgaaag tgattggcat
     5761 ggatgtcccc ggcaaagcgg atgcgcttgg tttaggctgg gtatatatgg ccccgaaaga
     5821 gggtcgtccg gggattattc agaagacagg cggtggtggc ggtttcatta cctatatggc
     5881 gatgatcccg cagaagaata ttggcgcgtt tgtggtggtc actcgttcgc cgttgacgcg
     5941 ctttaaaaat atgagcgatg gcatcaatga tctggtgacc gagctaagcg ggaataaacc
     6001 gttggttatc cccgcatcct gattaatact ctgaaggcaa acggttgata gcgaccagtt
     6061 tgcctttatt catttcgata tagccgcctt tacgcaaagc agcgagaact tctgcgacga
     6121 cagaacgcga tatgcgtgta cgttgatgga tatagttcat cacaccaata cgtgatcgca
     6181 attcttcatt ccagtcaatc attgataata atgttgcgcg gatttgttcg taggaattat
     6241 gcccaatgag ctgcacgtcg cgtaattcca gaattctatt ttgccaagct aaccagtaaa
     6301 aagcgtctcg ccagagttga ttttgttcaa taagcgtaat ggtttgtttg gctggtagat
     6361 gatatcccgt acaatttcct tctgatatta atttgtatgg tatatcgttt ttcattaaac
     6421 catcagccag ccccataata taaggggcct gggtaatacc gataagtacg ttttcttctc
     6481 tacgcagaga gataactccc tctaaaataa cgaaagtatc ttcgttattg acatcactgg
     6541 aaaatataac ttgcttttca ttattaaact cgaagcgcgt accgtatctg gacaaacatt
     6601 tatcgagctt accaaattcc tgaagaggtt taactacaga taacatttgc gcgtcctttg
     6661 cagtaatgcc cgtcaaatcc ttgacgggca ttatttagat taaattacca gtatttcttc
     6721 ggagtgaaga atattaccag gtatatttaa cacccacgtt cgcggaccag tcttgatcta
     6781 cgtcaccacc accgaggtag ttagcatcgg tataggcgct gaagttcttg gtgaagctga
     6841 actgagtacc cagaccaaca cgtaccgcag acccttcagt accgttatcg atggaatcac
     6901 cgttcacatc gttatcgttg ttagagtcgt cgtagacgta agccagtttg aagtacggag
     6961 tcagagcctg atcttcgctg taggtgaagg tataacctgc atctacaccc agttcataac
     7021 gcatgctgtc gtaagactga ccgtcaactt tcatgtcgtt gctcagctgg tagtcatcac
     7081 cagactggaa cagaccagaa acgctgccgt aaggagtcac gtaaccagca tcacccagtt
     7141 tgaagtcgta accggctttc aagccgaagc cccaagcgtc ggagttggtg ctaccgtcaa
     7201 cgtaagtacc gttgctcatg gttgcagaca ggtcgttgtt gaagtgagag tagctcaagc
     7261 taccatcaac aaagacgttg ttcgcgaagt gagcagaaga gtagatgtag gcagtctggc
     7321 tgtcttgatc tacctgacca gaacggtcat tcatgtcacc tttagcgaag cctgcagccg
     7381 caccgacgat ccacttagcg ttgttaccgt caactttggt atcaacaccg accatgatgc
     7441 cgttaacatc ctgatcatag ttgatggtgc cgttgtcgcc gttgaagtta ccaccgaagt
     7501 agcttaccca tgcgccgccg ttatcagcca ggccatgacg agagttggtc agacgagtac
     7561 caacggtgtc ttgttccagg ttccagatat tggtgttcgc agacgggatg ctcagcgcca
     7621 tgttagcgta gtcggtcagc tccatctgtt gcagaacaac ggtgttaccg cgctgttcag
     7681 cctgataggt gtatgcaccc aggtcagctt tgttagcagc agagaaggtc gcgttggttt
     7741 tggtatcatc gatatagatg agctgcttac ctgcgtaatc agcaatcgca ccagcgccag
     7801 tggagttgtc gatacgaact ttgtagttac cggttgcagc tacaacgttg tctgcagagc
     7861 tgttgtcaac ttcgtcgcca ttgttaatgt cgttgttacc attaacggtc aagtgaccat
     7921 cagagttcat cgcaataaca ccataaccgt agttagcttt gctggtgtcg ttggtacgat
     7981 cgttgacgag gtctgcattc aggatgtaat cattactatg aatgtcaaac acacctgcgt
     8041 aaacattacc gttgttatct ttaacgttgc cactggtcag ttccagagta tcagttgcaa
     8101 atacgctgga gatgtcattc atgttggtgt ttacatccag cacaccaccg ttagtaatgg
     8161 tattggcata cagcggtgca tatgcccaag ctgaagcagt atcttcaccg aagttaacgg
     8221 ttgagaaggt atcaatggtc aggtgatcgg tgttaacttc accgttaact tccattgaag
     8281 aaccaccgtt cagagtgatg gtgtcagaag tcagagaaga atctgcactc acgttaacct
     8341 gagaaccgct gttgatcgcc agggtatcga tcagggaagt tttcgtggtt tcccaatcag
     8401 aaccgttgtt cagaacaaca tcgaatacgc cgctctggaa tgcgttagcg ttatcaacac
     8461 gattccagcc atcttcagag taagaagcgt acgggttcag gctgtttggt gcaacgtcgt
     8521 agaaggcttc gtcagcaaca acaacgtcat aatcaatatt cgctgaacca acccatttgg
     8581 tgttgttcag agtcaggttc aggtgatcga cgttctgatc gtcagttgcc caaccgccat
     8641 gagtgtaagc agtggtagag ttgtcaatca ggtgacccgt cgcgtcaaag ttagcgttcc
     8701 aggtactgtc aaatacaacg tcaccaagca actgagagtt gttgaacgta acgttgttga
     8761 ccatacgata atcagaagtg tcatcatcga tgaaggctaa cgcaacatca tcagcaggag
     8821 atgcgccatt gccgtagtca cttggttcaa cagaattgcc gaaatgacca cgatctgatt
     8881 gttcgtcttc agtccatgaa ccagaagtta cagtagagtc attaacaacg atgttgttgt
     8941 cccactgatg accttcggta ttgtaggtcg cattaccgag gtcgtgttga accaaagtaa
     9001 tacctgcaac atgggagttg ttgctgatgt tgatgttact ttcaacatcc agagtcacag
     9061 cggtaccaag accagcggtt accaaacgat cataatctgt cttagatgtt ttaccgtctg
     9121 catccagata agtatcggtg aagtagaagg cgtcaaaatc atcgtcaata gtagaattag
     9181 cgatgttcag agtgaagatg tcgccatcaa cccagtcgtg gccagtataa tcgctaccgt
     9241 tggtccagac gtagtcgcca taaccaacct ggtaagatgt aaccatacca tgaacaattg
     9301 agttggagat gttcagtgta ttaccggtat ctgtgccata atcgtggttc agataataag
     9361 tagaaattac accatttaca gtgtcgccgc tgaaagtggt atcaaagtaa ccatcagctg
     9421 cgtggtcata tacataacca ccgtaagtca gataatcgtg atcaatttta gtgctgtcac
     9481 cccaattgta gatggtggct gcgtcatcgt gatacccgaa ggtgttataa gtagctgcat
     9541 tagcctgaga ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct
     9601 ttttccagga gtgcatttgt catccctcct cagggacgta ataagttgat ccatcaatta
     9661 tcaatgcata gaaaacatat tatgtttcgc gctaacgatt atgctgcgtt acagacgaat
     9721 ggttcaatta ttctttcccc taagtccgaa tgtggacata ta
//
LOCUS       DAZPAC010000150         9644 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZPAC010000150 DAZPAC010000000
VERSION     DAZPAC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.150"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..667)
                     /gene="ydcF"
                     /locus_tag="ACHUPM_003095"
     CDS             complement(<1..667)
                     /gene="ydcF"
                     /locus_tag="ACHUPM_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR0870801.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
     gene            complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="ACHUPM_003096"
     CDS             complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="ACHUPM_003096"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR0870802.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5042..5647
                     /gene="azoR"
                     /locus_tag="ACHUPM_003097"
     CDS             5042..5647
                     /gene="azoR"
                     /locus_tag="ACHUPM_003097"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR0870803.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5698..7014)
                     /locus_tag="ACHUPM_003098"
     CDS             complement(5698..7014)
                     /locus_tag="ACHUPM_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFR0870804.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7004..8761)
                     /locus_tag="ACHUPM_003099"
     CDS             complement(7004..8761)
                     /locus_tag="ACHUPM_003099"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR0870805.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8777..9644)
                     /locus_tag="ACHUPM_003100"
                     /pseudo
     CDS             complement(8777..9644)
                     /locus_tag="ACHUPM_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT         2448 a         2627 c         2273 g         2296 t
ORIGIN      
        1 tatcatcgcg taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc
       61 ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa
      121 cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga
      181 acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc
      241 gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg
      301 actggtcttc aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg
      361 cctggatggt cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg
      421 cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa
      481 tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg
      541 caaggatcac gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc
      601 actgtccgat aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa
      661 tgttcatggt tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc
      721 ggagcactct gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata
      781 tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt
      841 ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat
      901 gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc
      961 gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa
     1021 ctaacacgca actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt
     1081 gcaggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc
     1141 atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc
     1201 tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga
     1261 tataccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc
     1321 acccgcccct tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc
     1381 ttcgcaatat ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca
     1441 aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag
     1501 gagatacagt cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc
     1561 ttggctttgt ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc
     1621 agtagacggc gaagaccgtt ccacattacc tgtttttgct ccagcggatt atcaaacagt
     1681 ttgatcgcca cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg
     1741 ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagtccgctc
     1801 tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca
     1861 tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg
     1921 cggaaggtga ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg
     1981 gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc
     2041 ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt
     2101 accggtttcg gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc
     2161 tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc
     2221 acaccgtccg cgtccgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc
     2281 tgatgccaga agttcgggta atccagcttt ctgatttttt ccgccccttc tttgatcaac
     2341 atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta
     2401 tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacagc
     2461 gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg
     2521 gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc
     2581 agagcgtggc gaataaagag ttcacgacat aacgccgaat cgatctggct gtagttgacc
     2641 ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg
     2701 ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg ctgagcaacg
     2761 ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct
     2821 accagttccg ccaccattac ccatttcggc ggctttttga ataaaccaga accggggaag
     2881 atggagaaac gcgcgttacg tgcaccggta tattcctgtt tatcggcatc tttcatgccg
     2941 atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cacgatactc cgccggttcg
     3001 ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta gatatcctgc
     3061 cattcgcgca cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg
     3121 gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag
     3181 tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc
     3241 ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg
     3301 cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc
     3361 gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc
     3421 gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg
     3481 acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag
     3541 gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct
     3601 tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga
     3661 ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg
     3721 tagcggctga tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc
     3781 gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg
     3841 cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag
     3901 ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc
     3961 agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat cgcctgcaac
     4021 tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga
     4081 taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc
     4141 gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct
     4201 ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg
     4261 atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg
     4321 gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg
     4381 atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc
     4441 gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata
     4501 cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc
     4561 tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca
     4621 ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca
     4681 atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttcttc
     4741 acgccgtgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga atccagccgc
     4801 tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtagt
     4861 ctgcctgatt aattttcttt gaaatgatag atatagagta ccacatcacg gctttctatg
     4921 cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt
     4981 gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac
     5041 catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca
     5101 gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt
     5161 tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg
     5221 tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt ccgatgagct
     5281 gattgccgag ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa
     5341 catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg
     5401 ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag
     5461 ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt
     5521 cctcggcttt atcggcatta ctgatgtgaa atttgtcttc gccgaaggga tcgcatacgg
     5581 tccggaaatg gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc
     5641 tgcataaccg gacacgatct tgatgtggac tgtagtccag tccacatccc aatccactta
     5701 cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa tatgcgagcg
     5761 tctggctcga ataaagctaa tcgcttcatc aacggtttta cagtatccgt aacataacag
     5821 ccatgccgcc actaccagcg cactgcgcga taatcccagt gcgcaatgga ccagaacgct
     5881 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct ccccctcttc
     5941 cggaaccacc agatctagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc
     6001 acgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     6061 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     6121 acgcatagat agccacatcc cgatgcgcca gggcaatgtc agccagtaaa cggcgggcgg
     6181 gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     6241 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     6301 catcattagc tccatcaaca caatgcacgc gcacgcgcct acgacatatg gcagtgctat
     6361 tttgattcga cgttgatcag gtttctgata attccaacga cggtcgacgg gcaccatcca
     6421 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccatgt
     6481 cgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     6541 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     6601 agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc
     6661 actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc
     6721 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca
     6781 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg
     6841 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg ggaatatcat ggctattact
     6901 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6961 caacaataac cagccagctc cttgtagcaa tacgttccgg cgttcagtta tcacgacgca
     7021 ccgccatcga aaccgtaaaa atgccccact catcaatccg ttgtgtgcat ttatcaaatc
     7081 cagcatcatg cacgagcaaa tccatctccc cttgcgtacg cacgcgcatt acccacagtt
     7141 taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     7201 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagtgagt
     7261 tttttacctg ctcattttcg ggaaacagct catacagtcc agagacaatc gccagcgtag
     7321 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     7381 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     7441 tgtaatcacg taataaaata tcgcttacgg caggctcgtt tgccagtgca tccagtacat
     7501 agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg acgtggagat
     7561 cggcaacggc ttgtttaatc agtatttgta aatgagtttt gcgctggcga ataccgcgcc
     7621 agccaacact gttcaggtaa attttgtcga ttaagcgccc gaatgcgtta ctgccttgcg
     7681 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccagtg ctcattccga
     7741 gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc tttacgcata aagcgatacg
     7801 ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gaggcgccag cgatccgctg
     7861 atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta gcatataaac
     7921 gacgaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaacgtgt
     7981 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     8041 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     8101 ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttatag agatcgagca
     8161 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     8221 gccgatcgtg ggtcaaatag cgtcttttca cataggaatt aataaaaaac agaccacgca
     8281 gacgatgcca tagcgtcagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     8341 ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     8401 ccagcactgc gccgacgctt tgcgcgatca caaccacctc ttccagtcca acctggctgt
     8461 cgctggcagc aaaacggaca aattcatcga catcctgcac tgaacgcgca agagatgggc
     8521 tataaccacg cggccccgaa gtttttccat gcccccgggc atcccatgca taaaaagcag
     8581 tatctggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatggccac
     8641 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8701 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agagatgcgt gaattttcca
     8761 ttttctctat tttccttcag taacagcagt aacgaataaa ataaaaaaat accggagcgg
     8821 tgaaaattaa tgagtcaatc ctgtcaagga ttccgccatg tccaggtaat agtttcccgc
     8881 tatctttaac accaacatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     8941 taataccaat taacaaaccc gctaatattg cctgtaatgt atttagcggt gtcagtaacg
     9001 gtccaatgat catcgacgct atcatggtgg tgattacgcc gccgaccaga ccttccagcg
     9061 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     9121 actgtgcaat atcgtttgac gcggttaatg ccaaaagaaa taacaccagt aaggccccgc
     9181 cctggatatt catggatggc aacattaata actaggcggc atgactcaag gcgaaaacgg
     9241 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gggtcaccca
     9301 caaaaactcg ccaggcggct aatatcagaa acccagccaa aggtataaat aacagaaaca
     9361 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccacc
     9421 gcggaaaatg tacggatata agcatacaat attctttcaa tgccagaaaa cttatcagcg
     9481 caaaaaacgc caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     9541 tgccccacca ggtacgaata cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc
     9601 atagcagaaa acggtttatc agcgttgcta aaattaacaa cgcg
//
LOCUS       DAZPAC010000151         9599 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZPAC010000151 DAZPAC010000000
VERSION     DAZPAC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9599
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.151"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(56..1170)
                     /locus_tag="ACHUPM_003101"
                     /pseudo
     CDS             complement(56..1170)
                     /locus_tag="ACHUPM_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(1198..1818)
                     /locus_tag="ACHUPM_003102"
     CDS             complement(1198..1818)
                     /locus_tag="ACHUPM_003102"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR0870806.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(1815..2696)
                     /gene="rnm"
                     /locus_tag="ACHUPM_003103"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(1815..2696)
                     /gene="rnm"
                     /locus_tag="ACHUPM_003103"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR0870807.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            2834..2878
                     /gene="trpL"
                     /locus_tag="ACHUPM_003104"
     CDS             2834..2878
                     /gene="trpL"
                     /locus_tag="ACHUPM_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR0870808.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    2855..2948
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            2970..4532
                     /gene="trpE"
                     /locus_tag="ACHUPM_003105"
     CDS             2970..4532
                     /gene="trpE"
                     /locus_tag="ACHUPM_003105"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR0870809.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDNRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            4532..6127
                     /gene="trpD"
                     /locus_tag="ACHUPM_003106"
     CDS             4532..6127
                     /gene="trpD"
                     /locus_tag="ACHUPM_003106"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR0870810.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            6128..7489
                     /gene="trpCF"
                     /locus_tag="ACHUPM_003107"
     CDS             6128..7489
                     /gene="trpCF"
                     /locus_tag="ACHUPM_003107"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR0870811.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            7501..8694
                     /gene="trpB"
                     /locus_tag="ACHUPM_003108"
     CDS             7501..8694
                     /gene="trpB"
                     /locus_tag="ACHUPM_003108"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR0870812.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            8694..9500
                     /gene="trpA"
                     /locus_tag="ACHUPM_003109"
     CDS             8694..9500
                     /gene="trpA"
                     /locus_tag="ACHUPM_003109"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR0870813.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         2277 a         2560 c         2497 g         2265 t
ORIGIN      
        1 caacatacta cactatcttt atggttaaag cttgtttcga ttaacatcag cttaattacc
       61 agccaccacc gcctccgcca cccgcgccgc caccagcacc accaccggaa taacttcgcc
      121 ccaacaaact ggttttggcc cccagactca ggtgcatctg taatgaaaac aaaacgccac
      181 tacgggcaat ttccggggcg ctaacagccg ctgtgagtga tggggtgata cgtgctaccc
      241 attctcttcc aaggtttgct gagactgccc aacctagcaa cgcgtaatcc agtttccgtg
      301 tttccccttt ccgccgacgc ccactgtgtg ttgctgcctc tttacgcgca agatagttaa
      361 cgatagcttc accgtgggca taacgttgct gcccagtttg tgtaaacttt ggcagaaaaa
      421 tataccccat gccagtgaga taaccggtca ggaaaacagc gctaaaataa cccgctggca
      481 tataccagta aaagaataca tctccgacat tgatgtagag agaatagaag gccacgcctg
      541 aacaaatcag tggtaaacaa ttcttccgtc ctcaaggatg cggccatttc gatatgtgtc
      601 gccctcttct ccagtaaaga gatcgatttc gctaaatgga ttacccgcga tatcagggaa
      661 cttgagtgaa aatcgggtct tatagatttc aaacggccag tggttaccag tcacgttcca
      721 gattaacaga tctgaatctc cctcacgcag gaaagcattt ttaacctgat aatgaatttg
      781 ataatgatag tcgccttgag gcaagaaacg ttgtttatcg cccacgacaa tactcattaa
      841 cccgatatgc cagtcatgat gccagggttc aggaatacca tcacgggtga cgccaacaat
      901 atgatagttc agcagtgcag cgtccccacc aggctgcatc cagagtcgtg gtaaatcacg
      961 ataaaatccg tgtttaatct gctgatttcg cgcctgaaaa tgaatatttt ctttgatgtc
     1021 gagcacccca tccggttgca gagtggcttc aacatcaaag agcgaaatcc cttcttcata
     1081 agaagggatc tccgtgtttt ctgcataact tagagatgat acaaatagcc ctgctatcaa
     1141 cagcaaaatg cagcgaaact tccctgccat agtcttccat ccgtggaacg caatttgtta
     1201 taagaaaggc ttcacatcac ctacgccttc acgcaccacg accggcgtat catcggtaag
     1261 atcaataacc gttgtcggtt tctgcccgag atagccgccg tgaataatca aatctacctg
     1321 tttttccaga cggtctttaa tttcttcggg atcggattcg gtaaattcgc tgcctggcag
     1381 cattagcgaa gtggaaagca tcggttcgcc cagtgcttca agcaacgcct gcgcaatcgg
     1441 gttagaaggc acacgcatac cgatggtttt gcgtttttcc tgcaacagac ggcgtggcac
     1501 ttccttcgtc cccttcagga tgaaggtata gttgcccggc gtgttgtttt tcattaaacg
     1561 aaacgcaacg ttatcaacaa atgaataggt cgagagttca gaaagatcgc gacacatcag
     1621 ggtaaagttg tgaccgtccg gcagctggcg aatacgacaa atacgctcca tcgcgttttt
     1681 atcttcaatt ttacagccga gcgcatagcc ggaatcagtt ggataaacaa tcaccccgcc
     1741 tttacgcacg atctccaccg cctggttgat cagacgttgc tgtgggttat caggatgaat
     1801 ataaaaaaac tggctcataa ttccctctct gtggtgttct gcggctgttc ccatagctgc
     1861 cagacgcctt caacgcctgc gggcagccag agtttacgac ctagttcaat ccacgggcat
     1921 ggctgatgaa aatcagatcc ttgtgatgcc cataaatgat gctgacgcgc aagggccgcc
     1981 agctgggtac gttcattggg cgattgctgg cactgcgcga cttccatcgc gtcaccgtgg
     2041 tgttcggcaa aatgcgcaac caatcttttc agccatttag cactaagatt gtaccgccca
     2101 ggatgagcta gcaccgcctt accgccagaa tgatgaatga catcaatagc ttgttctatt
     2161 gtacaccact gtggcggaac gtatccggtt ttcccgcgcg ccagatattt tttaaagaca
     2221 tccgccattg aactggcttt gccgcacccc acaaggaaac gcgcaaaatg accgcgcgtc
     2281 accgcaccgc cctgcgccag tcgtcgcgcg ccttccagtg cccctggtat ttgcgctttt
     2341 tcaagtcttt cggcaatcag ttgcgcccgc tgattgcgcc gttctgtctg ctgcgcgagg
     2401 aactcacaca ttagcggatg agtaatatca atattcagcc cgacaatatg aatttcatga
     2461 ttttcccaga ccgtggaaat ttccacgccg ggaataagat tcagcgccag tcccgaacgt
     2521 gaaatttcct ctcttgctgg cacgattgca gctgtgttgt catggtcggt gatcgccagg
     2581 gtaccgacgc gcatctcgac tgcacggtgc accaatactt ctggcgtcag gcagccatcg
     2641 gaagctgtgg tatggctgtg caggtcgtaa atcactgcgt aattcgtgtc gctcaaggcg
     2701 cactcccgtt ctggataatg ttttttgcgc cgacatcata acagttctgg caaatattct
     2761 gaaatcagct gttgacaatt aatcatcgaa ctagttaact agtgcgcaag ttcacgtaaa
     2821 aagggtatcg acaatgaaag caattttcgt actgaaagct tggtggcgca cttcctgaaa
     2881 cgggcagtgt attcaccatg cgtaaagcaa tcagataccc agcccgccta atgagcgggc
     2941 ttttttttga acaaaattag agaataacaa tgcaaacaca aaaaccgact ctcgaactgc
     3001 taacctgcga aggcgcttat cgcgacaacc cgactgcgct ttttcaccag ttgtgtggga
     3061 atcgtccggc aacgctgctg ctggaatccg cagatatcga cagcaaagat gatttaaaaa
     3121 gcctgctact ggtagacagt gcgctgcgca ttacagcatt aggtgacact gtcacaattc
     3181 aggcactttc cggcaacggc gaagccctgc tggcactact ggataacgcc ctgcctgcgg
     3241 gtgtggaaaa tgaacaatca ccaaactgcc gtgtgctgcg cttcccccct gtcagtccac
     3301 tgctggatga agacgcccgc ttatgctccc tttcggtttt tgacgctttc cgtttattgc
     3361 agaatctgtt gaatgtaccg aaggaagaac gagaagcaat gttcttcggc ggcctgttct
     3421 cttatgacct tgtggcggga tttgaagatt taccgcaact gtcagcggaa aataactgcc
     3481 ctgatttctg tttttatctc gctgaaacgc tgatggtgat tgaccatcag aaaaaaaaca
     3541 cccgcattca ggccagcctg tttgctccga atgaagaaga aaaacaacgt ctcactgctc
     3601 gcctgaacga tcttcgccag cagctgaccg aagccgcgcc gccgctgccg gtggtttccg
     3661 tgccgcatat gcgttgtgaa tgtaatcaga gcgatgaaga gttcggtggc gtagtgcgtt
     3721 tgttgcaaaa agcgattcgc gctggagaaa ttttccaggt ggtgccatct cgccgtttct
     3781 ctctgccctg cccgtcaccg ctggcggcct attacgtgct gaaaaagagt aatcccagcc
     3841 cgtacatgtt ttttatgcag gataatgatt tcaccctgtt tggcgcgtcg ccggaaagtt
     3901 cgctcaagta tgacgccacc agccgccaga ttgagatcta cccgattgcc ggaacacgtc
     3961 cacgcggtcg tcgtgccgat ggctcgctgg acagagacct cgacaaccgc atcgaactgg
     4021 aaatgcgtac cgatcataaa gagctttctg aacatctgat gctggtggat ctcgcccgta
     4081 atgatctggc acgcatttgc acccccggca gcagctacgt cgccgatctt accaaagttg
     4141 accgttactc ttacgtgatg cacctggtct cccgcgtggt cggtgagctg cgccacgatc
     4201 tcgacgccct gcacgcttac cgcgcctgta tgaatatggg gacgttaagc ggtgcgccga
     4261 aagtacgcgc catgcagtta attgccgagg cggaaggtcg tcgccgcggc agctacggcg
     4321 gcgcggtagg ttatttcacc gcgcatggcg atctcgacac ctgcattgtg atccgctcgg
     4381 cgctggtgga aaacggtatc gccaccgtgc aagccggtgc tggcgtagtc cttgattcta
     4441 ttccgcagtc ggaagccgac gaaacccgta ataaagcccg cgctgtactg cgcgctattg
     4501 ccaccgcgca tcatgcacag gagactttct gatggctgac attctgctgc tcgataatat
     4561 cgactctttt acgtacaacc tggcagatca gttgcgcagc aatgggcata atgtggtgat
     4621 ttaccgcaac catattccgg cgcagacctt aattgaacgc ctggcgacga tgagcaatcc
     4681 gatactgatg ctttctcctg gccccggtgt gccgagcgaa gccggttgta tgccggaact
     4741 cctcacccgc ttgcgtggca agctgcccat tattggcatt tgcctcggac atcaggcaat
     4801 tgtcgaagct tacgggggct atgtcggtca ggcgggcgaa attcttcacg gtaaagcgtc
     4861 gagcattgaa catgacggtc aggcgatgtt tgccggatta acaaacccgc tgccggtggc
     4921 gcgttatcac tcgctggttg gcagtaacat tccggccggt ttaaccatca acgcccattt
     4981 taatggcatg gtgatggcag tacgtcacga tgcggatcgc gtttgtggat tccagttcca
     5041 tccggaatcc attctcacca cccagggcgc tcgcctgctg gaacaaacgc tggcctgggc
     5101 gcagcagaaa ctagagccag ccaacacgct gcaaccgatt ctggaaaaac tgtatcaggc
     5161 gcagacgctt agccaacaag aaagccacca gctgttttca gcggtggtgc gtggcgagct
     5221 gaagccggaa caactggcgg cggcgctggt gagcatgaaa attcgcggtg agcacccgaa
     5281 cgagatcgcc ggggcagcaa ccgcgctact ggaaaacgca gcgccgttcc cgcgcccaga
     5341 ttatctgttt gctgatatcg tcggtactgg cggtgacggc agcaacagta tcaatatttc
     5401 taccgccagt gcgtttgtcg ccgctgcctg tgggctgaaa gtggcgaaac acggcaaccg
     5461 tagcgtctcc agtaaatctg gttcgtccga tctgctggcg gcgttcggta ttaatcttga
     5521 tatgaacgcc gataaatcgc gccaggcgct ggatgagtta ggtgtttgtt tcctctttgc
     5581 gccgaagtat cacaccggat tccgccacgc gatgccggtt cgccagcaac tgaaaacccg
     5641 caccctgttc aatgtgctgg ggccattgat taacccggcg catccgccgc tggcgttaat
     5701 tggtgtttat agtccggaac tggtgctgcc gattgccgaa accttgcgcg tgctggggta
     5761 tcaacgcgcg gcggtggtgc acagcggcgg gatggatgaa gtttcattac acgcgccgac
     5821 aatcgttgcc gagctgcatg acggcgaaat taagagctat caattgaccg ctgaagattt
     5881 tggcctgact ccctaccacc aggagcaact ggcaggcgga acaccggaag aaaaccgtga
     5941 cattttaaca cgcttgttac aaggtaaagg cgacgccgcc catgaagcag ccgtcgctgc
     6001 gaacgtcgcc atgttaatgc gcctgcatgg ccatgaagat ctgcaagcca atgcgcaaac
     6061 cgttcttgag gtactgcgca gtggttccgc ttacgacaga gttaccgcgc tggcggcacg
     6121 agggtaaatg atgcaaaccg ttttagcgaa aatcgtcgca gacaaggcga tttgggtaga
     6181 aacccgcaaa cagcagcaac cgctggccag ttttcagaat gaggttcagc cgagcacgcg
     6241 acatttttat gatgcgctac agggtgcacg cacggcgttt attctggagt gcaagaaagc
     6301 gtcgccgtca aaaggcgtga tccgtgatga tttcgatccg gcacgcattg cctccattta
     6361 taaacattac gcttcggcaa tttcggtgct gactgatgag aaatattttc aggggagctt
     6421 tgatttcctc cccatcgtca gccaaatcgc cccgcagccg attttatgta aagacttcat
     6481 tatcgaccct taccagatct atctggcgcg ctattaccag gccgatgcct gcttattaat
     6541 gctttcagta ctggatgacg accaatatcg ccagcttgca gccgtcgctc acagtctgga
     6601 gatgggggtg ctgaccgaag tcagtaatga agaggaactg gagcgcgcca ttgcattggg
     6661 ggcaaaggtc gttggcatca acaaccgcga tctgcgtgat ttgtcgattg atctcaaccg
     6721 tacccgcgag cttgcgccga aactggggca caacgtgacg gtaatcagcg aatccggcat
     6781 caatacttac gctcaggtgc gcgagttaag ccacttcgct aacggttttc tgattggttc
     6841 ggcgttgatg gcccatgacg atttgcacgc cgccgtgcgc cgggtgttgc tgggtgagaa
     6901 taaagtatgt ggtctgacgc gtgggcaaga tgctaaagca gcttatgacg cgggcgcgat
     6961 ttacggtggg ttgattttgg ttgcgacatc accgcgttgc gtcaacgttg aacaggcgca
     7021 ggaagtgatg gctgcggcac cgttgcagta tgttggcgtg ttccgcaatc acgatattgc
     7081 cgatgtggtg gacaaagcta aggtgttatc gctggcggca gtgcaactgc atggtaatga
     7141 agatcagctg tatatcgata cgctgcgtga agctctgcca gcacacgttg ccatctggaa
     7201 agcattaagc gtcggtgaaa ccctgcccgc ccgcgagctt cagcacgttg ataaatatgt
     7261 tttagacaac ggccagggtg gaagcgggca acgtttcgac tggtcactat taaatggtca
     7321 atcgcttggc aacgttctgc tggcgggggg cttaggcgca gataactgcg tggaagcggc
     7381 acaaaccggc tgcgccggac ttgattttaa ttctgctgta gagtcgcaac cgggcatcaa
     7441 agacgcacgt cttttggcct cggttttcca gacgctgcgc gcatattaag gaaaggaaca
     7501 atgacaacat tacttaaccc ctattttggt gagtttggcg gcatgtacgt gccacaaatc
     7561 ctgatgcctg ctctgcgcca gctggaagaa gcttttgtca gcgcgcaaaa agatcctgaa
     7621 tttcaggccc agttcaacga cctactgaaa aactatgccg ggcgtccaac cgcactgacc
     7681 aaatgccaga acattacggc tggcacgaac accacgctgt atctgaagcg cgaagatttg
     7741 ctgcacggcg gcgcgcataa aactaaccag gtgctcggtc aggctttact ggcgaagcgg
     7801 atgggtaaaa ccgaaatcat cgccgaaacc ggtgccggtc agcatggcgt ggcgtcggcc
     7861 cttgccagcg ccctgctcgg cctgaaatgc cgtatttata tgggtgccaa agacgttgaa
     7921 cgccagtcgc ctaacgtttt tcgtatgcgc ttaatgggtg cggaagtgat cccggtgcat
     7981 agcggttccg cgacgctgaa agatgcctgt aacgaggcgc tgcgcgactg gtccggtagt
     8041 tacgaaaccg cgcactatat gctgggcacc gcagctggcc cgcatcctta tccgaccatt
     8101 gtgcgtgagt ttcagcggat gattggcgaa gaaaccaaag cgcagattct ggaaagagaa
     8161 ggtcgcctgc cggatgccgt tatcgcctgt gttggcggcg gttcgaatgc catcggcatg
     8221 tttgctgatt tcatcaatga aaccaacgtc ggcctgattg gtgtggagcc aggtggtcac
     8281 ggtatcgaaa ctggcgagca cggcgcaccg ctaaaacatg gtcgcgtggg tatctatttc
     8341 ggtatgaaag cgccgatgat gcaaaccgaa gacgggcaga ttgaagaatc ttactccatc
     8401 tccgccggat tggatttccc gtctgtcggc ccacaacacg cgtatcttaa cagcactgga
     8461 cgcgctgatt acgtgtctat taccgatgat gaagcccttg aagccttcaa gacgctgtgc
     8521 ctacacgaag ggatcatccc ggcgctggaa tcctcccacg ccctggccca tgcgctgaaa
     8581 atgatgcgcg aaaacccgga aaaagagcag ctactggtgg ttaacctttc cggtcgcggc
     8641 gataaagaca tcttcaccgt tcacgatatt ttgaaagcac gaggggaaat ctgatggaac
     8701 gctacgaatc tctgtttgcc cagttgaagg agcgcaaaga aggcgcattc gttcctttcg
     8761 tcacgctcgg ggatccgggc attgagcagt cattgaaaat tatcgatacg ctaattgaag
     8821 ccggtgctga cgcgctggag ttaggcatcc ccttctccga cccactggca gatggcccga
     8881 cgattcaaaa cgccactctg cgcgcctttg cggcaggtgt gactccggca caatgttttg
     8941 aaatgctggc actgattcgc cagaaacacc cgaccattcc cattggcctg ttgatgtatg
     9001 ccaatctggt gtttaacaaa ggcattgatg agttttatgc ccagtgcgag aaagtcggcg
     9061 tcgattcggt gctggttgcc gatgtgccaa ttgaagagtc cgcgcccttc cgccaggccg
     9121 cgttgcgcca taatgtcgca cctatcttca tctgcccgcc aaatgccgat gacgacctgc
     9181 tgcgccagat agcctcttac ggtcgtggtt acacctattt gctgtcacga gcgggcgtga
     9241 ccggcgcaga aaaccgcgcc gcgttacccc tcaatcatct ggttgcgaag ctgaaagagt
     9301 acaacgctgc acctccattg caggggtttg gtatttccac cccggatcag gtaaaagcag
     9361 cgattgatgc aggagctgcg ggcgcgattt ctggttcagc cattgttaaa atcatcgagc
     9421 aacatattaa tgagccagag aaaatgctgg cggcactgaa agtttttgta caaccgatga
     9481 aagtggcgac gcgcagttaa tccccctgcc gccagttccg ctggcggcat tttaactttc
     9541 tttaacgaag ccggaaaaat cctaaattca tttaatattt atctttttac cgtttcgct
//
LOCUS       DAZPAC010000152         9597 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZPAC010000152 DAZPAC010000000
VERSION     DAZPAC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.152"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..541
                     /locus_tag="ACHUPM_003110"
     CDS             <1..541
                     /locus_tag="ACHUPM_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR0870814.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
     gene            complement(597..1139)
                     /locus_tag="ACHUPM_003111"
     CDS             complement(597..1139)
                     /locus_tag="ACHUPM_003111"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0870815.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1123..1434)
                     /locus_tag="ACHUPM_003112"
     CDS             complement(1123..1434)
                     /locus_tag="ACHUPM_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR0870816.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(1619..2509)
                     /gene="cyoE"
                     /locus_tag="ACHUPM_003113"
     CDS             complement(1619..2509)
                     /gene="cyoE"
                     /locus_tag="ACHUPM_003113"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR0870817.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2521..2850)
                     /locus_tag="ACHUPM_003114"
     CDS             complement(2521..2850)
                     /locus_tag="ACHUPM_003114"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR0870818.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(2850..3464)
                     /locus_tag="ACHUPM_003115"
     CDS             complement(2850..3464)
                     /locus_tag="ACHUPM_003115"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR0870819.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3454..5445)
                     /gene="cyoB"
                     /locus_tag="ACHUPM_003116"
     CDS             complement(3454..5445)
                     /gene="cyoB"
                     /locus_tag="ACHUPM_003116"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFR0870820.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5467..6414)
                     /gene="cyoA"
                     /locus_tag="ACHUPM_003117"
     CDS             complement(5467..6414)
                     /gene="cyoA"
                     /locus_tag="ACHUPM_003117"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFR0870821.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(6875..8350)
                     /gene="ampG"
                     /locus_tag="ACHUPM_003118"
     CDS             complement(6875..8350)
                     /gene="ampG"
                     /locus_tag="ACHUPM_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR0870822.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(8394..8972)
                     /locus_tag="ACHUPM_003119"
     CDS             complement(8394..8972)
                     /locus_tag="ACHUPM_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0870823.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            9277..9594
                     /gene="bolA"
                     /locus_tag="ACHUPM_003120"
     CDS             9277..9594
                     /gene="bolA"
                     /locus_tag="ACHUPM_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR0870824.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
BASE COUNT         2630 a         2408 c         2402 g         2157 t
ORIGIN      
        1 gggcattatg tacgccaatg gtcagtatgt tgaggtagat tgtaagaaag cgaaggaata
       61 tcttgataaa ggtgtccaca tttatggtgg cccggaagac tttctggcta cttgtcgaaa
      121 agacatgatt gacagaaaaa cggttgacga tacgttaccc gtgattacgg ttacccgttc
      181 tgggatgcga gataattttt tagataaagg tttttcctgc atggatagcc tcttcgccac
      241 caccaataaa ttaggcgaag tggccaatct tcgcgtgaca ttcagcattc gtcgcccatc
      301 cggtaaggag attaaccaaa cggttggctt cgcgcctttc ggtttaaacc ggctgaatat
      361 tagctttact gattacctat ttggttcatt taccagtaat tcgtctctta ttctttataa
      421 accggaattt gagcgaaaat cttgtgcaac tgtaaggacc acaatcgttg ccgcaacggc
      481 aaccattaac ggcaaggatg tggagttgct gaaagcgggg gcaattgaac aaaagtggta
      541 acgctgctca gcccccggct aaggcaaaac ttgccctggc gggtggcgat aataatctaa
      601 gccgcgagaa tatgctgaat cgccaaaaac ataggtacag caccaaaagc atttggtgtc
      661 gctgaaagct gaacaaagcc gagacgagca taaaaattaa tggcggcagg gtcagcatca
      721 agataaaccc ctttaattgg taatgcctgg tgaattattt ttacatgttc aaaaaaatca
      781 catagtaggt cctgaccaaa gccccgcttt tgatacttcc gtgctacccc caacatgact
      841 aatctgacaa caccaatttc tgaaggttgt gaaccctgaa ggacgccaga aacgcgttgt
      901 ttttccagcg aatatgccgt aaaagtacag atgccgatca gttcaccact ttgtctgtca
      961 ataagtgctt tagccgcaca gtcgctatta cgcacacttt tctttagcga tgcgcgtaca
     1021 aatttatcga taacagggtt accgcaatca aactgttttt gcccaggata agtaatatca
     1081 gactgataat tacaggagag gactatgggc agagtaatct cttcatgctt gtcgaccatc
     1141 gctgtttttc cttttcatta acgccttgag ggcgagcgtc ggttgagcag gttcagcgat
     1201 gagttggttc atcagttccc agctttgatt cgaaagctca cggcgacgtt ggttatcaag
     1261 cacactttcg gcgcgttcca tagcagcgct caaaataaaa gcgcttaaat ctaccccaac
     1321 ggcttgcgca gcttcacgaa gtttgttttt ggcgtcggga ctagttttga gttcgacccg
     1381 cgcatttttc agcgttttat cgacatcggg tagagtggct gtttttccag gcattatgac
     1441 ctctttgatt aactgaatga aatcgtaggg agtcgatctc atacataata cggctatcaa
     1501 ccgtatgatt aatttatgtt catctgctca ctctgtcaag acatacggtt attagccgta
     1561 tctctgatct cagacaataa aaaaggtgcc gtagcacctt tttaatagag aggttgtgtt
     1621 accaaacagc agccagcagc gtatgcgagt ccggcaccat aaaatcaacg gacatcatca
     1681 ccgagagggc tgtgatggcg atgatagaga agccgaacag cttgcgcgcc cagatcctgt
     1741 catcagcaac tttataaccg cgcagggcca tacctaacca ccagacgctc accgccgcgg
     1801 cgaccaccag atatttatac ccagcgtaac cgccaagaga gagcatcagc gtggcaacgg
     1861 caaaggcgat gatatacagc gtgatgtgat tcttcgccac cgaaatgcct tttaccactg
     1921 gcaataccgg aatgtttgcc gcctgataat ccttaaagcg gaaaatggcg atggcatagg
     1981 agtgaggcat ctgccacagg ctgaagatag ccagcaggat cgctgcgccg ctatcgaact
     2041 cgccggttac tgcacagtag ccaatcaccg gcggcgcagc gccggagagc gaaccaatca
     2101 atgtgccgta gaccgagtgg cgtttcatgt acaggctata aacgccgaca taaaccacaa
     2161 agcccatcac ccccagccag caggccagcg gattcgcgcc aaaccacagc agcataaagc
     2221 cagcaaagcc tagcaaggtg gcgtacacca gcgagacagc aggagagatc aggcctttaa
     2281 ccagcacccg attcttcgtc ctttccatct ttctgtcgat atccctgtcg atgtagttgt
     2341 taaacacaca acccgacgcc acaaccagtg acaccccaac cagcgtgtag ataaacaggg
     2401 gataatcaat gctgcccttt gaggccagca ggaatccccc aatcaccgag atcaggttgc
     2461 caaagatgat gcctggtttc gttacttgca ggtattgctt aaacatcata accgccgctc
     2521 ttagtgcatc atcatgttgt agttgaggtt ccacataatc cagatggagc ctacaaccag
     2581 gatagcgatg attagcacgg tgaagacaaa cgccgtcatg ttccagcctt catctgattt
     2641 ggtattcatg tgcaggaagc acaccagatg caccagaacc tgtaccactg ccattgccag
     2701 gattgttccc agaattacgg ccggagaggc agctcctgtc atcaccatcc agaacggaat
     2761 caccgtcaga atgatcgaca ggataaagcc tgtcatgtag gtttttacgc tgccatggga
     2821 cgcgccgctg tgatcggtag aatgactcat tacatcgccc ccatcagata aacaacagtg
     2881 aacacacaga tccaaaccac atccaggaag tgccagaaca ggctcaggca catgatgcgg
     2941 gtacggttag tgctggtcag gccgcgacga gcgatttgta ccatcagcac cgccatccag
     3001 ataagaccgg aagtgacgtg cagaccgtgc gtgccgacca gcgcaaagaa cgctgacagg
     3061 aagccgctgc gatccggacc catgccgtta acaatcaggt gatggaattc gtagatttcc
     3121 atcccgataa atccggcacc aaacaaccag gtcaacgcca gccaggagat aacctggctt
     3181 ttgttgtttt tgtacatggc gatagccgcc atgccgtagg tgatggagct gaacaacagc
     3241 aagaaagttt caaccagaac gaacggcagt tcgaaaatgt ccttacctgt cgggccgcct
     3301 gcggtgccgt tcaccagaac ggcataggta gcaaacaaga ttgagaacag aatgcagtcg
     3361 ctcatcaggt agatccaaaa tccgaagatt ttggttccgc ctgcatcgtg gtgcccgtgt
     3421 tcgtgcgcgt gggcagtcgc gtgcgtcaaa gtatcagttg ccatttttca gccctgcctt
     3481 agtaatctca tcgaaatgct ggttttccag tttttcgatt tctgccaccg gcacgtagta
     3541 atccacgtcc tcgtcgaagc ttttcacgat ccaggtgatg atcatgcctg cgaagccaac
     3601 aatcgccagc caccagatat gccagatcat ggcgaaaccg aagatggtgg agaaagctgc
     3661 aatgacgata cctgcaccgc tgtttttcgg catatgaatt tcttcatagt ggtcaggctt
     3721 tttatacgct tcgcctttct ctttcatttc ccagaatgca tcacgctcgt gaacgtgcgg
     3781 cacaacggca aagttataga acggaggcgg ggaagaggtt gcccactcca gcgtacggcc
     3841 accccacggg tcgccagtca ggtcacggtt ctggtcgcgg tcgcgaatag aaacgtacat
     3901 ctgaataacg aggcagagaa tacccagcgc aatcagtact gcaccgctag ctgcaatcat
     3961 cagcatggtg tggaactgcg ggtcaatctg ctggctcaaa cgacgggtca tgcccatgaa
     4021 gcccagcgca tacagcggca taaaggcaac gaagaagccg atgatccaga accagaacgc
     4081 gcgtttaccc caggtttcat tcagtttgaa accgaacgct ttaggccacc agtaggtcat
     4141 ccctgcgaag cagccgaaga ccacgccgcc gatgataacg ttatggaagt gcgcaatcag
     4201 gaacaggctg ttatgcagaa cgaagtccgc gcccggtact gccagcagca cgccagtcat
     4261 cccgcccacc gagaaagtga cgataaaacc gatggtccac agcatcgcag aatggaacac
     4321 gatgcggccc tgatacatgg tgaacagcca gttgaagatc ttcaccccgg tcgggatggc
     4381 gataatcatt gtggtgatac caaagaaggc gtttacgttc gcgcccgcac ccatcgtaaa
     4441 gaagtggtgc agccaaacga tgaacgacag cacggtgata cagacggttg cccataccag
     4501 cgaggtataa ccaaacagac gtttacgcga gaaggttgcc gcaatttcgg agaacacacc
     4561 gaaaacaggc aggatcagga tgtaaacttc cgggtggccc caggcccaaa tcaggttgat
     4621 gtacatcatc atgttgccac ccatatcgtt ggtaaagaaa tgggtgccca gatagcgatc
     4681 cagggtcaac aacgcgacgg taaccgtcag aattgggaag gaagcaataa tcagtacgtt
     4741 cgcgcacagt gatgcccagg taaatactgg catcttgaac atggtcatgc ccggtgcgcg
     4801 catcttcaga atggtaacga agaagttgat accggtaagc gtcgtaccta taccggatag
     4861 ctggagactc catatccagt aatcgacacc gactcccgga ctgtactcta ttcccgatag
     4921 cggtggatag gccagccagc cggtctgcgc aaattcgccc acgccgagag aaacgttaac
     4981 cagaatcaca ccaacaacag taaaccagaa gcttaagttg ttgaggaacg ggaacgcaac
     5041 gtcacgcgcg ccgatctgca gcggaaccac caggttcatc agaccgataa cgaaaggcat
     5101 cgccacgaag aagatcataa tcacgccgtg cgcggtgaag atctgatcgt agtggtgagg
     5161 tggcaggaag cccgcttcgc ccgccgaggc aagagcctgc tggctacgca tcataatggc
     5221 gtcagcaaaa ccacgcagca acatcacaat cgccacgatg atatacatga taccgaggcg
     5281 tttatggtcg acggaggtca gccactcttt ccacaggtag gtccacttac cgaagtaagt
     5341 gatcaggcca acgagcgcca gacctcccaa aataatgcca gcgatcgtaa ccatgacgat
     5401 aggttcatgg aacgggactg catcaagtga taattttccg aacatcttta ttcttcctca
     5461 acccctttaa tgggcggatt ccgcgtggct catgtccatg ccttccatac cttcgtgtgc
     5521 actgtgctca ccttctggct gggtcatgtc catgctctta ccgtgagcca taaacttgtt
     5581 aatcacatcg gcaaacaagt ctggtttcac gttggagaaa tattccacct ggttgtattc
     5641 gctaggcgcg gccagttttt cgaacgcagc catgtcagac atggtgttcg gcgactgttt
     5701 cgcttttgcg acccattggt cgaattcggc gcgatccggt gttgcaatag ctttgaactt
     5761 catgcctgag aagcccggcc cgctgtagct ggcggagata ccgtcataag taccgggttc
     5821 gttggcgatc agatgcaggc gagtctgcat accggccatg gcataaatct ggctacccag
     5881 acgcggaatg aagaaggagt tcatcacgga gttggaggtc actttgaagt acaccggagt
     5941 gttcgccggg aaagcgattt cattcacggt tgcaatgccc tgttccgggt agatgaagaa
     6001 ccatttccag tccatggaaa ccacttcgat ggtaatgggc ttctcgtcgt gtgccagcgg
     6061 cttgctaggc tcaagagcgt gagtggtttt ccaggtcagt accgcaagga agatgatgat
     6121 taagataggt accgtccaga ccacagcttc cactttattg gagtgtgacc agttcgggct
     6181 gtacttagca tctttattgc tcgcacgata cttccaggcg aaaccaacag ccatcaagat
     6241 tgcgggaata acgacaatca acatcaggcc aaatgccgtc agtatcagtg aacgttgctc
     6301 cagaccaatc tgtcctttgg gatctaacag cgcagaatta cagccactga gcaatacagt
     6361 gcctgcaaat aatgacaacc atcccaaact tttattgtat ttcctgagtc tcatttaacg
     6421 acctcaattc cacgggatct ggtggcgttt aaagtgtgtg ggcattttac ggggaggtta
     6481 cattactgta aacatgatta aattcctgtt acttggattt ggctgtcagg ttacatatgt
     6541 tgcaaaacat atcaagttga ttttaagaag ctgttgcaaa agggcgttat aacgaaaggg
     6601 gagtgcagaa ggagtttgga cgggcaattg gtataaccaa tcttgaataa aacaattgtt
     6661 taacaaataa ttatcattat ggtgacaatt gggtgggggg aaaataaaga ctcattagct
     6721 gaatcgtgta agaaaaaata gaattcataa attgcgtcaa taatttccaa acgctaagcc
     6781 gcacaaaaga acaaatatta ttaagcgata ctgatgttta tttagccgta ataattacgg
     6841 cgagtgatta ctacagctaa ataatattta cagattacgt cagatgcgtt tttcgtagag
     6901 ccagataatc cagcaaacca ccaagcacga cgccagaaag ggcgactaaa accccgactt
     6961 ccagcagagc aggcaggaaa gagaagtgcg tcaaatccag cgcgtccatc gtcaacagca
     7021 gtaaccacac ggccaacagg ctgacgcccg ccgccagtgt ccacatggca aaggcataac
     7081 ctgtcggata ttcggtacgg gagataaagt tgtcatttac tcgtgtatat tcaagcgtct
     7141 ggcggcaaac cagcagcaaa ataagccccg gtacggcagc ggcgacggag aatagataga
     7201 atgtcgacca gccgtgtgct tcaacaaacc aacccgccac ggggccgaca taaactcgtc
     7261 ctacagcgga aagcgctgag agcagggcaa attgagtagc ggaaaatgac ttattacaca
     7321 gcgtcattaa cagtgcgaca aaggctgatg tgcccatccc gccacagagg ttttcgaaaa
     7381 agacggctgc gcccatgctg tagagatgct tatcagtaat cgacagcagc cagtaaccag
     7441 cgttagacgc accttgtaaa atgccgaaaa tcagcagcgc ccggaacagc gacaggcgct
     7501 gcatcaaaat cccaccgtac aatgcgccca caatggtcgc cagtaagcca agcgttttgt
     7561 taaccacgcc tacttcaccc gcatcaaacc cgacgccgcg aatcaaaaac gtggttgtca
     7621 ggctcatggc gaatgcgtcg cccagcttat acagcacgat aagaagcaaa ataagccagg
     7681 cattattgcg accaaagaaa tctcgcagag gtgcaacaac cgcttgttcc agcgtttttg
     7741 gcacaggaat ggtgtcggtc ggttctggtg caagcaacgt cgcgataata caagggatca
     7801 acagcgccgc catcaaccag tacatgccct gccagcccag ccatttatct gccagccaca
     7861 gggctaagcc gccggaaacc agcatcccta aacggtaacc cagcacgctg attgccgcgc
     7921 ccgcaccacg ttcttctgcc gggagcacat cggttttcca cgcatcgaag acaatatcct
     7981 gggaggccga gcaaaaagcg atcaccaccg ccagtgccgc catccagcgg agttgggtgc
     8041 caggttcgag aaaacccatc gccgcaatgg cgactaataa caggatttgc gtggcgagca
     8101 gccaaccgcg ccgccgccca aaaaatgggg gcgtgtagcg gtccatcagc ggtgaccaga
     8161 ggaatttaaa aacgtaagcc tggcctacca gagagaagaa accaatggtt ttgagatcaa
     8221 tattctcgac cgtcatccag gcctgtaagg taccggatgt cagggcgagc ggtagcccgg
     8281 aagcaaaacc caggatcagc aatatggctg aacgcggctg ttgaaaaata cgtaaatatt
     8341 gactggacat gggcgtctac aggcccggtt tgcaccgggc cagagggcag aaattaacgc
     8401 gcgttctgct tgatgaattc gtggatgctg gtgtcctgag acatatcagc gatggtatcg
     8461 gtcagcacac tgttaaccgc atcggcgata tttttgttgg aggcctggaa cgcaccttca
     8521 acgttgtagc tggcacgata gtttttggtc attttgttgc cattctgcgc ggtagcgatg
     8581 atggcgatat ccgctttggt cgcgatgttg tagcgcacgt tgccctggga cacgtcagca
     8641 tacagttggc taacgatgat ttgcagatta accgggccat tcggaccaac catgtaacca
     8701 cgcgcggtca tctgtttttc cagcacttct tgcagcagga aacgcagatc tcgggaggcg
     8761 gtcagggtaa cgatttgatt atcgcgggtg acttttgcca gcgcctgatc ggtacgctga
     8821 tcggcaccat taatgcttac ggtgacgccc atcaggcttg gatcctgctg tggcagtgta
     8881 atcgtcgggg aaacttcaat agttgttggc ggttttgcgc atcctgcaag cataaacaga
     8941 gcaactaacg ggaagaggat ttttttgaac atgttcgggc tctcagagac tcttaagcgt
     9001 gtttggtaaa aattcccgcc atcataacat tgccaacggc gaggggaagt gggtaaggca
     9061 tgtaaattca tcatgttggc gaaataatca cccctggtaa aagaaacgct gatgcgaggc
     9121 ctgtgtttca atctttaaat cagtaaactt catacgcttg acggaaaaac caggacgaaa
     9181 cctaaatatt tgttgttaag ctgcaatgga aacggtaaaa gcggctagta tttaaaggga
     9241 tggatgacat ctcagcgttg tcggaggaga tatttcatga tgatacgtga gcggatagaa
     9301 gaaaaattaa gggcggcgtt ccaacccgta ttcctcgaag tagtggatga aagctatcgt
     9361 cacaatgtcc cagccggctc tgaaagccat tttaaagttg tgctggtcag cgatcgtttt
     9421 acgggtgaac gttttctgaa tcgtcatcga atgatttaca gtactttagc ggaggaactc
     9481 tctactaccg ttcatgcgct ggctctgcat acttacacta ttaaggagtg ggaagggttg
     9541 caggacaccg tctttgcctc tcctccctgt cgtggagcag gaagcatcgc gtaaaaa
//
LOCUS       DAZPAC010000153         9542 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZPAC010000153 DAZPAC010000000
VERSION     DAZPAC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.153"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1321
                     /locus_tag="ACHUPM_003121"
     CDS             <1..1321
                     /locus_tag="ACHUPM_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0870825.1"
                     /translation="PQFRIPAELIQHDIDFVAAHGVKFEYGCSPDLTVEQLKNQGFHY
                     VLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVVVVGAGNTAMDCAR
                     AALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNPERFDADGTLTLRV
                     MSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMGVPLDKNGWPDVDH
                     NGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSHQNDKYWNNVNPAE
                     IYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPNRANVSIAVPGFQN
                     RFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSSTPGFLVEDCRVRV
                     RLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRVEV"
     gene            1324..2652
                     /gene="ssnA"
                     /locus_tag="ACHUPM_003122"
     CDS             1324..2652
                     /gene="ssnA"
                     /locus_tag="ACHUPM_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFR0870826.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2703..3482
                     /gene="ygfM"
                     /locus_tag="ACHUPM_003123"
     CDS             2703..3482
                     /gene="ygfM"
                     /locus_tag="ACHUPM_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFR0870827.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3479..6418
                     /locus_tag="ACHUPM_003124"
     CDS             3479..6418
                     /locus_tag="ACHUPM_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFR0870828.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6351..6665
                     /locus_tag="ACHUPM_003125"
     CDS             6351..6665
                     /locus_tag="ACHUPM_003125"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR0870829.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6701..7051
                     /gene="ghxQ"
                     /locus_tag="ACHUPM_003126"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6701..7051
                     /gene="ghxQ"
                     /locus_tag="ACHUPM_003126"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7120..7608)
                     /locus_tag="ACHUPM_003127"
     CDS             complement(7120..7608)
                     /locus_tag="ACHUPM_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0870830.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="ACHUPM_003128"
     CDS             complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="ACHUPM_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFR0870831.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2301 a         2559 c         2488 g         2194 t
ORIGIN      
        1 tcctcagttc cgtattcctg cagagttaat tcagcacgat atcgattttg ttgccgctca
       61 cggcgtgaaa tttgagtatg gctgctcacc cgatttaacc gttgaacagt taaaaaatca
      121 gggcttccac tatgttctga ttgccaccgg cactgataaa aatagcggtg tgaaactggc
      181 gggcgacaac caaaatgtct ggaaatcact ccccttcctg cgtgaataca acaagggtac
      241 agcgctcaag ctgggcaaac atgtggtcgt tgtcggggcg ggtaacaccg caatggactg
      301 cgctcgtgcg gcgttacgcg ttccaggcgt agaaaaagca acgatcgttt accgtcgttc
      361 actacaagag atgcccgcat ggcgcgaaga gtatgaagaa gcgttgcacg acggcgtaga
      421 gttccgtttc ctgaataatc cggaacgttt cgatgctgat ggcaccttaa ccttgcgcgt
      481 tatgtcgctt ggcgaaccgg atgagaaagg tcgtcgccgt ccggttgaaa ccaacgaaac
      541 agtaacactg catgtagaca gcctgatcac cgccattggt gaacagcagg atactgaagc
      601 cctgaatgcg atgggcgtgc cgctggacaa aaacggctgg ccagacgtcg accataatgg
      661 cgaaactcgt ctgactgacg tctttatgat cggcgacgta cagcgcggac catcctccat
      721 tgtcgctgct gtcggaaccg cgcgtcgggc gaccgatgcc atccttagtc gggaaaatat
      781 ccgttcccac cagaacgata aatactggaa caacgtcaat ccagcggaaa tctatcaacg
      841 taaaggcgat atctctatca cgctggtgaa cagtgacgat cgtgacgcgt ttgtcgccca
      901 ggaagccgct cgctgcctcg aatgtaacta cgtttgcaac aagtgtgtgg atgtctgccc
      961 gaaccgcgcc aacgtctcca ttgcagtccc tggcttccag aatagattcc agacgctgca
     1021 cctcgacgct tactgtaacg aatgcggcaa ctgcgctcag ttctgtccgt ggaacggtaa
     1081 accatacgaa gacaaaatca ccgtcttcag cctggcgcaa gactttgata acagcagcac
     1141 cccaggcttc cttgtggaag attgccgggt acgagtacgt ctgaataacc aaagctgggt
     1201 gttaaacatc gacagcgaag gtcagtttaa caacgtacca ccggagctga acgatatgtg
     1261 ccgcatcatc agccatgtcc accagcatca tcattatctg ctgggccgcg tggaggtgta
     1321 atcatgttga ttctgaagaa tgtcaccgca gtgcagttac acccggcgaa agtgcaggaa
     1381 ggcgttgata tcgctatcga aaatgacgtg attgtcgcta tcggcgatgc cctgacgcaa
     1441 cgctaccccg atgccagcta caaagagatg catggtcgga ttgtgatgcc gggaattgtc
     1501 tgctcgcaca accattttta ctcggagctt tcccgcggaa ttatggcaaa catcgcccct
     1561 tgcccggatt tcatctcaac gctgaaaaat ctctggtggc ggctcgatcg cgcccttgat
     1621 gaagagtcgc tctattacag cggactgatt tgttccctgg aagcgattaa gagcggatgt
     1681 acatcggtta tcgatcacca tgcctcgccg gcgtatatcg gcgggtcgct ctccacattg
     1741 cgcgacgcat ttttaaaagt tggcctgcgc gcgatgacct gttttgaaac tactgaccgt
     1801 aacaacggca tcaaagagtt gcaggaaggt gtagaagaaa acatccgttt cgcccgtttg
     1861 attgatgagg cgaagaaagc gacaagcgag ccgtatctgg tggaagcaca tatcggtgct
     1921 cacgcgccgt ttaccgtgcc ggatgccggt ctggagatgc tgcgtgaagc cgtgaaagcc
     1981 acaggccgtg gtttgcatat tcacgctgcg gaagaccttt acgacgtttc ctacagtcac
     2041 cactggtacg gcaaagacct gctggcacga ctggcgcaat tcgacctcat cgacagcaaa
     2101 acgctggtcg ctcatgggct gtacttgtcg aaagatgaca tcgccctact caatcagcgc
     2161 gatgcgttcc tggtgcataa cgcccgttca aacatgaaca accatgtcgg ctacaaccat
     2221 caccttagcg acatccgcaa cctggcattg ggaacggacg gcattggttc ggacatgttt
     2281 gaagagatga aatttgcctt ctttaaacat cgcgatgcgg gtggtccgct gtggcctgac
     2341 agttttgcca aagccctgac taacggtaac gaactgatga gccgcaactt tggcgcgaaa
     2401 tttgggcttc tggaagccgg ttacaaagct gatttgacca tttgcgatta caactcgccg
     2461 acgccgctgc tggcagacaa tatcgccggg catatcgctt tcggtatggg ctcaggcagc
     2521 gttcacagcg tgatggtcaa tggtgtgatg gtctatgaag accgtcagtt taacttcgat
     2581 tgcgattcca tttatgcaca agccagaaaa gccgctgcca gtatgtggcg tcggatggat
     2641 gcgctggcat aaatgacaga tgccctcttc ccgcagggaa gagggttaaa taaggaaaga
     2701 agatgattga acaatttttc aggcccgact ctgtcgaaca ggcgctggaa ctgaagcgcc
     2761 gctaccagga tgaagccgtc tggttcgccg ggggcagcaa actcaatgct acaccaaccc
     2821 gtaccgataa aaagattgcc atttccttac aggatctgga actggactgg gttgactggg
     2881 ataacggtgc actgcggatt ggtgcaatgt ctcgcttgca gccactgcgt gatgcgcgct
     2941 ttattcctgc agcactgcgt gaagccctcg gttttgttta ctcacgccat gttcgtaatc
     3001 agtcgaccat tggtggtgaa atcgccgccc ggcaggaaga gtcagtgctg cttcccgtcc
     3061 tgctagcact ggatgctgaa ctggtttttg gcaacggcga aacgctgtca atcgaggact
     3121 acctggcctg cccatgcgat cgcctgctaa ccgaaattat cattaaagat ccgtatcgca
     3181 cctgtgcgac ccgcaaaatt agccgttctc aggcaggttt aaccgtcgtg acggcagccg
     3241 ttgcaatgac agaccacgac ggtatgcgaa ttgcgctgga tggcgtcgcc agtaaagcac
     3301 tgcgtctgca cgatgtcgaa aaacaaaatc tggaaggcaa tgcacttgaa caggctgtcg
     3361 ccaacgccat tttcccgcag gaagatttgc ggggcagcgt ggcctataaa cgctatatca
     3421 cgggagttct ggtagccgac ctgtatgccg actgccaaca ggctggggag gaagccgtat
     3481 gatcatccac tttactttaa atggcgcgcc tcaggagcta accgttaatc caggcgaaaa
     3541 cgtgcaaaag ctgttgttta acatgggaat gcactctgta cgcaacagtg atgatggctt
     3601 cgggtttgcc ggttctgacg caataatctt taacggtaat atcgttaacg cgtccttgct
     3661 tattgccgca cagttagaga aggcagatat tcgtaccgca gaatctctgg gtaaatggaa
     3721 cgagttaagt ctggttcaac aggcaatggt tgatgttggc gtggtgcagt ctggttataa
     3781 cgatccagct gcagctctga ttatcaccga tcttctcgat cgcatcgccg cacctacccg
     3841 cgaagagatc gacgacgcgc tttctggttt gttcagccgc gatgcaggct ggcaacaata
     3901 ctatcaggtc attgaactgg cggttgcacg taaaaataat ccgcaggcca ccattgatat
     3961 cgctccgact ttccgtgacg accttgacgt cattggcaag cattatccta aaactgatgc
     4021 cgcgaaaatg gtgcaggcga aaccctgcta tgttgaagac tgcgtaacgg ctgacgcctg
     4081 cgtcattaaa atgttacgta gcccacacgc tcacgcactg attactcatc tggatgtcag
     4141 caaagctgaa gccttaccgg gcgtcgttca cgttattact cacctgaatt gccctgatat
     4201 ctactatacc ccggggggtc agagcgcacc ggaaccgtca ccgcttgacc gccgtatgtt
     4261 cggcaagaaa atgcgtcacg tcggcgatcg tgttgctgcg gtcgtcgcag aaagtgaaga
     4321 aattgcgctc gaagcactga agctcatcga cgttgaatat gaagtgctta agccggtaat
     4381 gtcgatcgac gaagcaatgg cggaagatgc gcctgtcgtg cacgatgaac cggtggtgta
     4441 tgttgctggt gcgccagata ctctggaaga cgataacagc catgcagccc agcgcggcga
     4501 gcatatgatc atcaacttcc cgatcggttc tcgccctcgc aaaaatatcg ccgccagtat
     4561 tcatggtcat attggcgata tggacaaagg ctttgccgat gccgatgtga tcattgagcg
     4621 aacctataac tcaacgcagg cacagcagtg cccgactgaa acacatatct gctttacccg
     4681 gatggacggc gatcgtctgg ttatccacgc ctccacccag gtaccatggc acttacgccg
     4741 ccaggtcgcg cgcctcgtgg gcatgaaaca gcataaagtt catgtcatta aagagcgagt
     4801 tggcggcggt tttggttcca aacaggacat cctgctggaa gaagtgtgcg cctgggcaac
     4861 ctgcgtgacc gggcgtccgg tactattccg ctacacccgt gaagaagagt ttattgctaa
     4921 cacctctcgt cacgtcgcga aagtcaccgt caaactggga gcgaaaaaag atggtcgcct
     4981 gacggcagtg aagatggatt tccgcgccaa cactggccct tacggcaacc actcactcac
     5041 cgtaccgtgt aacggaccgg cgctgtcgct gccgttatat ccgtgcgata acgtcgattt
     5101 ccaggtcacc acctactaca gcaacatttg cccaaatggt gcttatcagg gttatggcgc
     5161 accgaaaggt aacttcgcta tcaccatggc attagcggaa ctggctgaac agttacagat
     5221 cgaccaactg gaaattatcg aacgtaaccg ggtacacgaa gggcaagagc tgaaaattct
     5281 cggtgcaatc ggtgaaggta aagcgccgac ctccgttcct tccgccgcca gctgtgcact
     5341 ggaagagatc ctgcgtcagg gtcgcgagat gatccaatgg tcgtcaccga aaccgcaaaa
     5401 tggtgactgg cacatcggtc gcggcgtcgc cattatcatg cagaaatcgg gtatcccgga
     5461 tatcgatcag gctaactgca tgatcaaact ggaatcggac ggcaccttta tcgttcattc
     5521 tggcggtgcg gatattggta ctggtctgga tactgtagtg acgaaactgg cagcagaagt
     5581 gctgcactgc ccaccgcagg acgtgcatgt tatctccggt gataccgatc atgcgttgtt
     5641 tgataaaggc gcatatgcct cgtccggtac ttgcttctcg ggtaacgcgg cgcgtttggc
     5701 agcggaaaat ctacgggaga aaattctgtt ccacggcgcg caaatgttgg gtgaggcagt
     5761 ggcagatgtt caactggcaa cgccgggcgt cgtgcgcggc aagaaaggcg aagttagttt
     5821 cggggatatt gcccataaag gcgaaaccgg catcggcttt ggttcactgg tgggaactgg
     5881 cagttatatc acgcctgatt tcgccttccc gtatggcgca aacttcgctg aagttgccgt
     5941 caacacgcgt acgggtgaaa tccgcctgga taaattctac gccttgctgg actgcggtac
     6001 accggtcaac ccggaattag cattgggaca aatctacggt gctaccctgc gagctatcgg
     6061 ccacagtatg agcgaagaga tcatttatga cgccgaaggt cacccgttaa cgcgtgattt
     6121 acgcagttac ggcgcaccga aaattggtga cattccgcgt gatttccgcg ctgtgctggt
     6181 gccgagcgac gataaagtcg gcccgttcgg ggcgaaatcg atctcggaaa tcggtgtaaa
     6241 tggcgcagct ccggcgattg ctaccgcaat tcacgataca tgcggcatct gggcagaaga
     6301 aagtcaaagt gggcatggga acggatatcg gtgccggaac aactttcaac atgctgcaaa
     6361 cgctgaacga agcctacaaa gtgttgcaat tacaaggcta tcgcctctcg gcatatgaag
     6421 cgttttacct ggccacgctc ggcggagcga aatctctggg ccttgacgat ttgattggca
     6481 actttttacc tggcaaagag gctgatttcg tggtgatgga acccaccgcc actccgctac
     6541 agcagctgcg ctatgacaac tctgtttctt tagtcgacaa attgttcgtg atgatgacgt
     6601 tgggcgatga ccgttcgatc taccgcacct acgttgatgg tcgtctggtg tacgaacgca
     6661 actaataata aaactttaac atcctcgtga ggacatcatt atgtctggag acatcctaca
     6721 aacaccggac gcaccaaagc cacagggcgc gctggataat tattttaaaa ttaccgctcg
     6781 tggcagtacc gttcgtcagg aagtactggc tggcttaacg acctttctgg ccatggttta
     6841 ttccgttatc gtcgttccgg gaatgctggg caaagcagtt tttcctcccg cagctgtgtt
     6901 tgttgccacc tgtctggtcg cgggcttcgg ctcgttgctg atggggttgt aggccaattt
     6961 gccaatggcg attggttgtg cgatttcctt gacggcattt accgcattca gtctggtact
     7021 cgggcaacaa attagcgttc ctgtcgcact ggtcgcattt tacgcgggtg gttgggcaat
     7081 ctaatagatt ctccgcgcct ttcagcgcgg agatttcttt cacagaggat tctcaccgct
     7141 ggcaacaata cgctgctggc ggactcgcct gagttctctt tcagtaagca actgcagcgc
     7201 ctgtgtcggg caagattcaa cacaggctgg cccctcttcc cgctgttcac agagatcgca
     7261 tttcacaatt tgttgtcggg tatcccccgg caacgactga atggtgatca ttccaaacgg
     7321 gcacgcactg acgcaactct gacagccaat acaacgggca gaattggtct gcaccacctg
     7381 ctcgcccatc gtcagcgccc ccacggggca agcgccaaca caaggggcgt tttcacactg
     7441 atggcacatc accggcgcac tgatgctgtc cagtcgctgt accttcagcc ggggtaaaaa
     7501 gacatcggca ttcaactcct gttctgaagg gtgagcgacc acacaggcta cttcacaagt
     7561 acggcagcca atacagtcag ccggattaac gataattaac gatttcatca cgatgccttc
     7621 gtatcaaaca gagttaacat atcgcgcgcc gcctgccttc ctgcggccat tgcagtgaca
     7681 accagatccg cgccatgaac tgcatcacca ccagcaaaga cttttttcag atgcgtctgg
     7741 gtaggtaaat acccgacgtc accggtttga atcaggcccc atttatcgag tttaattccg
     7801 ctgccctgca accacggcat ggcatgcgcc tggaaaccaa aggccataat cagaacatcg
     7861 gcgggcaatt caaactctga acccgctacc ggacgaggac gacggcgacc atccggcccc
     7921 ggctcaccca tggcggtacg aatcaggccc accgcagtta aacgcccatc ttcgtcacaa
     7981 gcgatatatt gcggttgaac attgaactga aactcgacac cttcctcgcg cgcattaacc
     8041 acctctttgc gcgagcccgg catactgact tcatcacgac gatacgcgca ggtcacgctg
     8101 gcagcattga ggcgaatgga agtccgcaaa caatccattg tcgtatcgcc accgcccaat
     8161 accacgactc gcttaccttc cacgtccgtc agcggatact cttcagactc cggcaatccc
     8221 atgagctggc gggtatgggc agtcaggaac ggtagagcct gaatgacacc gggcgcatct
     8281 tcatgcggca gatctgctcg catcatcccg taagtcccca cgccgatgaa aactgcatca
     8341 tattcagaag ttaaatcgct aaaggtaata tcgcggccaa tttcacagtt aagatgaaag
     8401 tcgattccca ttgcggtgaa tatctctcgc cgctggctta ataccgtttt atcgagtttg
     8461 aaaggaggaa tgccaaaagt cagcataccg ccaatttctg gatggcgatc aaagacatca
     8521 acctgaactc ccgcgcgcgc cagaatatca gcacacccta accctgctgg cccagcgcca
     8581 atcaccgcca ctttttcgat acggggaaca actttgctga catcgggacg ccagcccatc
     8641 gccagcgcgg tatcggtgat gtagcgttcc agattaccga tagagactgc gccagagtga
     8701 tctttcaaag tacatgcgcc ttcacaaaga cggtcctgtg ggcataccct gccgcagatt
     8761 tctggtaagg aactggtctg gtggcaaagt tctgccgctt caataatctt tccttcctgt
     8821 accagacgga tgtaatccgg aatagcgtta tgcagcgggc aatgccagtt gcagttagct
     8881 ttttcggcac aataaacaca gcggtcactc tcataagtcg cttgcagtgg atccagcccg
     8941 caatagattt cgccaaagtg ggttttccgt tcactcgctg aaattttatc tgcgccttta
     9001 cgcgagttaa cggggagcaa cgctgcactg cgagatggct gagcgtctga tgacgctttt
     9061 cctgctgccg ttttgcgctg gcgagccacc tttatccact gtaacccttt atcgtccatc
     9121 agtcgtaacg cctgtgttgg gcagacttca atacaggctt gcgtgccgga actgcgctgg
     9181 ttacaaaggt cgcatttctg cgcaatcgta tcgaccatct caacgacgcc aaaggggcaa
     9241 gcgattgcgc atcttttaca gccaatacat ttttgctcgt ccagttgtac gctatcggac
     9301 tggaaagtca gagcattaac cggacaagcc gtaacacaag gggcattgtt gcagtgatgg
     9361 caggccaccg gattcgcagc ctggcctttc cctacaacgt ggatacgcgg tcgaaagtca
     9421 ctgtgactca gcggccagtt ttcttgattg tgtgccaccg cacaggcaat ttcacaagca
     9481 tgacagccta tacattccgc agcttcagca gcgataaact tattcatttg catccctttc
     9541 at
//
LOCUS       DAZPAC010000154         9500 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZPAC010000154 DAZPAC010000000
VERSION     DAZPAC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.154"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..108
                     /locus_tag="ACHUPM_003129"
     CDS             <1..108
                     /locus_tag="ACHUPM_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0870832.1"
                     /translation="HRTIIAFAVDDNKVFIAGVYHGGQIYETDFLSSAP"
     gene            complement(149..1231)
                     /locus_tag="ACHUPM_003130"
     CDS             complement(149..1231)
                     /locus_tag="ACHUPM_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFR0870833.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3060)
                     /locus_tag="ACHUPM_003131"
     CDS             complement(1228..3060)
                     /locus_tag="ACHUPM_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR0870834.1"
                     /translation="MARMFDTLGFYIMNVQHFDFFTDNEFSNLERYQTCKAMAKGYAG
                     NYKTGQSRLMHARSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAE
                     RTGELIAQFPAEDAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDF
                     SHASVIDPACGGGAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLV
                     LLESVLMPLCVKVKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKY
                     SRSLFGHANLYGLFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYA
                     LDFVTDRDGVFDDVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKG
                     GATWLLPRVKDDACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPL
                     VWAESITSAGFRFSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAI
                     LPQGFIDETGGVVVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVS
                     AYELNSIPLPSLEQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="ACHUPM_003132"
     CDS             complement(3275..3631)
                     /locus_tag="ACHUPM_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870835.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="ACHUPM_003133"
     CDS             complement(3689..4111)
                     /locus_tag="ACHUPM_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0870836.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="ACHUPM_003134"
     CDS             complement(4127..4852)
                     /locus_tag="ACHUPM_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFR0870837.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="ACHUPM_003135"
     CDS             complement(4874..5620)
                     /locus_tag="ACHUPM_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0870838.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="ACHUPM_003136"
     CDS             complement(5627..6691)
                     /locus_tag="ACHUPM_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFR0870839.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGGPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="ACHUPM_003137"
     CDS             complement(6763..7188)
                     /locus_tag="ACHUPM_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR0870840.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="ACHUPM_003138"
     CDS             complement(7185..7448)
                     /locus_tag="ACHUPM_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0870841.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="ACHUPM_003139"
     CDS             7560..8060
                     /locus_tag="ACHUPM_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0870842.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="ACHUPM_003140"
     CDS             8080..8274
                     /locus_tag="ACHUPM_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR0870843.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="ACHUPM_003141"
     CDS             8274..8564
                     /locus_tag="ACHUPM_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0870844.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8844..8996
                     /locus_tag="ACHUPM_003142"
     CDS             8844..8996
                     /locus_tag="ACHUPM_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR0870845.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            9008..9373
                     /locus_tag="ACHUPM_003143"
     CDS             9008..9373
                     /locus_tag="ACHUPM_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870846.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
BASE COUNT         2443 a         2493 c         2016 g         2548 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatc tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ccggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggcc ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatac tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaaat aatgatgcta ttgtttcagg cgtcaaccca ccccgcccca cagaatgcag
     8701 ggcaatactt cgagttacca gacagtggtc aggggttaag tagccagccc gaggcgtaag
     8761 aacatgacgg cagggttcaa tctttaacta tgcagcaggt tttttgttcc gccaccccgg
     8821 cgttaagggg aaatgaggtc agcatggata ctatcgatct tggcaacaac gaatctctgg
     8881 tgtgcggcgt gttccctaac caggatggaa cgtttaccgc catgacgtat accaaaagca
     8941 aaacgtttaa aaccgaatct ggcgcgcgtt gctggttagc cagaaacact gactgatgag
     9001 gctgacgatg gaatttaaag atttaccgtc tgatgtacag aaaacagcag ctcatacatt
     9061 gcattctgtg ctgcgagaaa tcgggaaaga tattgcaagc gagccagcaa aagatctggc
     9121 ccggaaaatc aagaccgctt tcgttgagct ttataatgtt ggcactgact ctgaaactgt
     9181 cgagaccaag accgtaagtt caccaatatt ctcacttgag ccaagcgtat tatcaggtga
     9241 aatatgcacc gagatttcca gcgaactgct tccggtaata cgagaagcaa ttcgccgtcg
     9301 cggtttggat ggaagttatg atcatgacgt tctgcaagtt ctcaggacaa tggtgacttc
     9361 actggggatt tgatccctgc gtcctctaag cttttgatat agcggtcctc caagaagcgg
     9421 taaattttgt cgaaatcatc ttttccctct ggtttattca gaggattaca ggacccgtaa
     9481 gtagcggcat acatttcaac
//
LOCUS       DAZPAC010000155         9432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZPAC010000155 DAZPAC010000000
VERSION     DAZPAC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.155"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            205..1071
                     /gene="folD"
                     /locus_tag="ACHUPM_003144"
     CDS             205..1071
                     /gene="folD"
                     /locus_tag="ACHUPM_003144"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR0870847.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHUPM_003145"
     CDS             1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHUPM_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR0870848.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1393..1914
                     /locus_tag="ACHUPM_003146"
                     /pseudo
     CDS             1393..1914
                     /locus_tag="ACHUPM_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHUPM_003147"
     CDS             complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHUPM_003147"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR0870849.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHUPM_003148"
     CDS             3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHUPM_003148"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR0870850.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHUPM_003149"
     CDS             4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHUPM_003149"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR0870851.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4846..5355
                     /gene="purE"
                     /locus_tag="ACHUPM_003150"
     CDS             4846..5355
                     /gene="purE"
                     /locus_tag="ACHUPM_003150"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR0870852.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5352..6419
                     /gene="purK"
                     /locus_tag="ACHUPM_003151"
     CDS             5352..6419
                     /gene="purK"
                     /locus_tag="ACHUPM_003151"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR0870853.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6556..7449)
                     /locus_tag="ACHUPM_003152"
     CDS             complement(6556..7449)
                     /locus_tag="ACHUPM_003152"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR0870854.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7446..8099)
                     /locus_tag="ACHUPM_003153"
     CDS             complement(7446..8099)
                     /locus_tag="ACHUPM_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR0870855.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8110..9369)
                     /locus_tag="ACHUPM_003154"
     CDS             complement(8110..9369)
                     /locus_tag="ACHUPM_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR0870856.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2102 a         2673 c         2498 g         2159 t
ORIGIN      
        1 tagacaagga aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg
      121 aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac
      181 gtaacagatg gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg
      241 cagcaggtgc gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg
      301 gcaccaggac tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca
      361 agcaaacgca aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa
      421 accaccagcg aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc
      481 gatggcattc tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa
      541 cgtattcacc cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc
      601 cagcgcgcgc cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt
      661 tacaacattg ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt
      721 cgtccaatga gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc
      781 actaaaaatc tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag
      841 ccgggcttta ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc
      901 aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc
      961 gcctcataca ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt
     1021 gaaaacacgc tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac
     1081 attttcttta ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg
     1141 gagcgaaagc ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg
     1201 cgcggttgaa acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg
     1261 tcacagcgta caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc
     1321 ttttcagact tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc
     1381 gaagcgacca tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta
     1441 gggttgagtt cgaaagtcat ccctccgcgt ctgttatttg cctgaatcat cctggcgatg
     1501 ctgcccgacg ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt
     1561 catcgcgggt ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg
     1621 attggacgcc gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg
     1681 ttgctatcgc acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg
     1741 tggccgtggt cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt
     1801 gcattgtctt gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg
     1861 gtgtggttgc cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat
     1921 gcgaaaactc gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg
     1981 ccatcttcca gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc
     2041 cagtctttcg ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact
     2101 tcgctgtcgt ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg
     2161 cctaatacag cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct
     2221 tctgctttca gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg
     2281 gtgttgaaat cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca
     2341 ggagcaacgg ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc
     2401 gcctgcttca ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg
     2461 aagtaacgca cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta
     2521 cccagcgatt tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag
     2581 ttaacatact gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg
     2641 aacatcaggt cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc
     2701 attgccgaac attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc
     2761 ggttcgccct ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg
     2821 accacgtcaa cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca
     2881 taagtcggat cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga
     2941 cctttggcga tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc
     3001 ggctccatat ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc
     3061 atgcgatcca ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg
     3121 tcgtcgatat cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag
     3181 cgcgcaacca cgtcaaaagc aacaaaggta cgcccgtgac caatatgaca gagatcgtaa
     3241 acggtgattc cacacacata catgccgact tccccggcgt gaataggctt aaattcctct
     3301 ttttggcgtg tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg
     3361 ggtaattgag ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat
     3421 gatccgatcg tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag
     3481 caccaatcaa acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat
     3541 tgtcatcaaa acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg
     3601 ccgcgaaggt ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca
     3661 gggcggcggt tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga
     3721 agccaacaac ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc
     3781 gcactctgca actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc
     3841 tggcgaaagc ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga
     3901 cgtggtagac aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc
     3961 aaaagaagac gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt
     4021 attgcagatc ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt
     4081 ttagcggggg aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg
     4141 tggattggcg acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg
     4201 gtgtccgatt ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc
     4261 aaacgctttg cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt
     4321 tatggccgcc gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat
     4381 caggcttttc gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg
     4441 ttgtttgtgc gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc
     4501 agtaaatcgc tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa
     4561 catcaggtgc aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc
     4621 gccaaccagc aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg
     4681 gtgaaagtca cggcggatga cgttgagctg attcattttc cgttttaaaa aacccgcaac
     4741 tttgctgatt tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt
     4801 gccctctaaa gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat
     4861 aatccggcgc gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc
     4921 gccgccgaaa tcttcgaaat cctgaatgtc ctgcaccacg ttgaagtggt ttctgctcac
     4981 cgcacccccg ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg
     5041 attattgcgg gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg
     5101 gtgccggtgc tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac
     5161 tccatcgtac aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc
     5221 gcggcaaacg cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac
     5281 cagcgtctga atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg
     5341 cgaggtacgg catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc
     5401 gtcaggcagg cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg
     5461 cggcggtgcc ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg
     5521 cattaacccg cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta
     5581 ttgctgaccg tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt
     5641 ggcagttact tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg
     5701 cgattgttaa gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca
     5761 atgaaaccga acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta
     5821 acttctctgg tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt
     5881 atacgctgac gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc
     5941 aggccaacgc acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc
     6001 tgggctatgt gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca
     6061 acgaactggc tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca
     6121 gccagtttga gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga
     6181 ataatccgtc ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat
     6241 tgccgctggt gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc
     6301 atctgaattt gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc
     6361 cgctgctgcc gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag
     6421 caggtgctca tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt
     6481 acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc
     6541 cggatgctct catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc
     6601 aattcgcgat aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac
     6661 cgccgttacc ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg
     6721 tgtggcatgg cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc
     6781 atcggtgagg atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag
     6841 atctttatca atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc
     6901 gccactgcaa atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag
     6961 aattttacgc ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat
     7021 ctgccagccg taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat
     7081 aaatttctct ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac
     7141 cgtcgtaaca ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat
     7201 catcccctgg ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc
     7261 cagattctgt aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg
     7321 ataagaacgg gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt
     7381 ttccgccgtc agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag
     7441 tgttttcatg gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct
     7501 gcccctgacg tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg
     7561 caactcagag catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa
     7621 cgtaaataac tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa
     7681 aactggcgta actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc
     7741 ccggtttcct ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc
     7801 gtaatacgca gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc
     7861 ccttgagcca caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat
     7921 tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt
     7981 aacagttcgc cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc
     8041 caggactggc gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt
     8101 gcgcgttcct caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca
     8161 cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc
     8221 gataccggta ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc
     8281 caggcacgcg ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta
     8341 aatttccgcc atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa
     8401 gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat
     8461 cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc
     8521 ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc
     8581 gaacatattg ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc
     8641 agcatccatc gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac
     8701 gctgaggaaa tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg
     8761 tggggccagc gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc
     8821 gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc
     8881 cagcgccgcg cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac
     8941 atcttcgccg taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta
     9001 cgcccggttg ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat
     9061 accgcccatt ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg
     9121 ctccagtatt gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc
     9181 cacgcacgcc cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg
     9241 aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag
     9301 ccagtgtgga cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga
     9361 tgtaaacatg ggaacccctt attgcaaacg ttctaataaa cgagccaggt aatgactcca
     9421 acttattgat ag
//
LOCUS       DAZPAC010000156         9409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZPAC010000156 DAZPAC010000000
VERSION     DAZPAC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.156"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..168
                     /locus_tag="ACHUPM_003155"
     CDS             <1..168
                     /locus_tag="ACHUPM_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR0870857.1"
                     /translation="NRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFG
                     MTPGEYRRKFR"
     gene            complement(340..669)
                     /locus_tag="ACHUPM_003156"
     CDS             complement(340..669)
                     /locus_tag="ACHUPM_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR0870858.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(674..859)
                     /locus_tag="ACHUPM_003157"
     CDS             complement(674..859)
                     /locus_tag="ACHUPM_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR0870859.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(856..3495)
                     /locus_tag="ACHUPM_003158"
     CDS             complement(856..3495)
                     /locus_tag="ACHUPM_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR0870860.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3703..4692
                     /gene="ldhA"
                     /locus_tag="ACHUPM_003159"
     CDS             3703..4692
                     /gene="ldhA"
                     /locus_tag="ACHUPM_003159"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0870861.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4803..5225
                     /gene="hslJ"
                     /locus_tag="ACHUPM_003160"
     CDS             4803..5225
                     /gene="hslJ"
                     /locus_tag="ACHUPM_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR0870862.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5222..5488)
                     /locus_tag="ACHUPM_003161"
     CDS             complement(5222..5488)
                     /locus_tag="ACHUPM_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR0870863.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5762..9286
                     /gene="nifJ"
                     /locus_tag="ACHUPM_003162"
     CDS             5762..9286
                     /gene="nifJ"
                     /locus_tag="ACHUPM_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR0870864.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2338 a         2459 c         2302 g         2310 t
ORIGIN      
        1 aaccgtcgtc tcgatttttg tgcagatgcg attcgccatg ccgcagatga tgaaaaactg
       61 gcaggcatcg gctttcattg gggattttct gaccagagtc atttttcaac ggtatttaag
      121 caacgctttg ggatgacgcc aggcgagtat cgacgtaaat tccgttaact aattcgcgtt
      181 gtgtgtactg caacgccaat tagttaaaac agatatttga ctatgcattt ttcagggcaa
      241 aaggcaattt gacaggagtt tttgaatatt caccttgttt caggaaaata gatcaatcgc
      301 tttctcctgt agcgttatcc cgatattgcg gttttgctat taaaattttc gcacccattt
      361 accgtttatc ccttgtacat attcccccgg tttggcccga gctacgagct tttgcccggc
      421 gagtttcgcg atatcatcta ccgacacatt attttgcttt gccagttgtt ggtaactcgc
      481 tttacgttcg gcattaatgt cggttactaa tttctctgtt tcagcatccg ttttcagcgc
      541 aaccagataa ccataaaatg tttcacctac ccgcccctga gttctggctt catccagagt
      601 caatgccatt acattgctgc ttaccagccc aacaagaaac gcacaaagaa gtaatgtttt
      661 cttcatcatc gcctcagaaa agatcgctac gagtttcaag cagctcttcg acatctttgt
      721 ctgccttgat gatgatctca tgctcaattt taacgttcat attgatagtg atcggttcct
      781 tgggcgcagc gacttcaata cgcggcgtac agccaaccag cataaaagat gacgtcaacg
      841 cagccagtaa aattttcatt gtttttcctc acactctttt ccttgcggac agtcatttcc
      901 cggcagacgt gcgttctgct ccaaccatgc ctggagatta tcgccaaagc gtaaactgcg
      961 ccacagcgta aaaatattct cttcatgatg gtaattaagg tttaccgtac cgcttttacc
     1021 atcaacgcga ctggtacctg taatgttggc ctgcatggtt aataaaccga gattatctaa
     1081 gttaattttt gtcgatgacc ggctaatttc catatagcgc aaccagttaa ttgctgaacc
     1141 cgcagtcata ttgtctttca ccaccgcatc tgcggtgtct ttatccagcc gcaaggtcat
     1201 tggcccgcta tttgccagcc aaccatcttt cactatccat ttttcgttgt ttagccataa
     1261 cggcaatgca ccactaaatg ccccggacat ggcgaattgt ttcggattga cggcgctaac
     1321 cagttcgctg gatgagagat tattcagccg caacagcgcc gggtcatgtt gcggcatacg
     1381 taattgtttc atcagtacgt taccgcctaa cacatcgacg ctaacatcag tcaacagcaa
     1441 gggttcttct tcagtccacg gataacgccc ttgcaaatca gccgtaatat ttttcgctgt
     1501 caccagatta atcacttcgg caattcgcaa cgtaacgggg ccgcgagtcc ccagatgcca
     1561 ggctccatcg gcaaaacgga aaggcaggac aaaatcgaca ccgttaacct gattatctgg
     1621 catccaggca ctaccgcctt tcaacacgcc gtgtcctccc gcgcggaatc cttgttcagg
     1681 cgcagcagaa aatgcaacct gagcatacag ttccccatcg cgtaagttca tcttccagtc
     1741 gggtggcacc agcggctgga atacagtcag tgattgtttt ggccaccagg cgttaccgcg
     1801 cagacgaata ccgtcccagc gaccatttac ccgaaccggg ccaatctcac cagcatgtaa
     1861 atcgccttta aagaggaaat acgtcggatc gcgcccatcg acgctaaatt ttaaggttga
     1921 tggcggtaac acactgccgc cagtgaacag cgtttgcccg gcgtccagtg acagcgcgcc
     1981 gctgaaggag ggatgctgtg cgtcacgtac ccagacgacg ggcttgtcga gaattaatcg
     2041 cggcttttct accgtcatcg taccgtattg taactggtcg aaaccggtgg agaggtcggt
     2101 cagcgtaatc gtgctgtcat gccattcacc ttttcctgcg acatcccagg tggcatttat
     2161 cggtgtaaaa ctgccttttc cccagtagcg ccactgccag cggcccgcgt cagggagaaa
     2221 atcattcgcc agcccatcca tatgcagcac gaaatcgccc agttcatatt catgcgcctg
     2281 caaaatggcc tgcaaacgtc cgtcaacacc acgttgggtg acttttacac ccgctaaagg
     2341 ccagcggatt tcatcgatgt ccagcgaatc gatgactctt cccttcgaac gtaataacgc
     2401 gcctggctca aaggccagcg ttgggtcagt cagacttcca cttagctgcg cgggtaagcg
     2461 ggcatataaa atgaggtccg cctgtttcgc ttcaccggtc agctgcagag gcagctggct
     2521 gttatccatg cttaattttc ctgggccaaa atttagcacc gcgttgcctt tacccgcttg
     2581 cccctgggtc agcacactca gtcgtccgct gatcagagcg ttctcaagcc ctgcctgcca
     2641 gttgtcgact ttgacaccca gtcggccact caaaggaaaa cctgcatacg gccaactcca
     2701 gcgaccatcg cttacggtca attgttgacg agtaatttgc cacggcaaat cgagcaacgg
     2761 atcgccgtta tcccgtgcca gcacaatcaa ttgcccgcta ttttcctgcc agtccagctc
     2821 tgcatccacc agtgacggtt cctgcggcaa gtttaacgtc gcagtagcat gcccacttac
     2881 aggaagtcca tccggcacga gcggcatagt aaattccccc accagtttaa ccggcggctg
     2941 attttcaaac gcgatgacat ccagttcgct gactgtaagt tgttgccctt tcagctggcc
     3001 ttgaaattta actttttcgc cctgataacg cagttgctga atatcagagg ttaatgcgag
     3061 agagagtttt ccctgccatt cctgccaggg agaaaaaatc agtttatcga tattgatcca
     3121 ggtgttaggc agcatggact gccactgcgc gagggttttt ggagcagctg gcgattgctc
     3181 cgtctgcggc aatttcgcca gacaagcaga atcaagttct accatgccga cgttcaataa
     3241 ccagcggctg ggatgtgaaa ggctggcgtt ggtgatatgc gcaagctgac aatctcccac
     3301 cagataacgg agatcgggaa tgattaaacc tttacgcgta atgcgtgggc tttcatctaa
     3361 tgcaatacgt gtcccgagcg gtagccagat gcccgccagc gtgggaaccc acagcccgag
     3421 cgtcatcagc agcgtcaacg gcacaagaat aatcagtaat aacagcgcga gaacggcttt
     3481 atatttaccc agcatgggta gttaatatcc tgatttagcg aaaaattaag cattcaatac
     3541 gggtattgtg gcatgtttaa ccgttcagtt gaaggttgcg cctacactaa gcattgttgt
     3601 tgatgaattt ttcaatatcg ccatagcttt caattatatt tgaaattttg taaaatattt
     3661 ttagtagctt aaatgtgatt caacatcact ggagaaagtc ttatgaaact cgccgtttat
     3721 agcacaaaac agtacgacaa gaagtacctg caacaggtga acgagtcctt tggctttgag
     3781 ctggaatttt ttgactttct gctgacggaa aaaaccgcta aaactgccaa tggctgcgaa
     3841 gcggtatgta ttttcgtaaa cgatgacggc agccgcccgg tgctggaaga gctgaaaaag
     3901 cacggcgtta aatatatcgc cctgcgctgt gccggtttca ataacgtcga ccttgacgcg
     3961 gcaaaagaac tggggctgaa agtagtccgt gttccagcct atgatccaga ggccgttgct
     4021 gaacacgcca tcggtatgat gatgacgctg aaccgccgta ttcaccgcgc gtatcagcgt
     4081 acccgtgacg ctaacttctc tctggaaggt ctgaccggct ttactatgta tggcaaaacg
     4141 gcaggcgtta tcggtaccgg taaaatcggt gtggcgatgc tgcgcattct gaaaggtttt
     4201 ggtatgcgtc tgctggcgtt cgatccgtat ccaagtgcag cggcgctgga actcggtgtg
     4261 gagtatgtcg atctgccaac cctgttctct gaatcagacg ttatctctct gcactgcccg
     4321 ctgacaccgg aaaactacca tctgttgaac gaagccgcct tcgatcagat gaaaaatggc
     4381 gtgatgatcg tcaataccag tcgcggtgca ttgattgatt ctcaggcagc aattgaagcg
     4441 ctgaaaaatc agaaaattgg gtcgttgggt atggacgtct atgagaacga acgcgatcta
     4501 ttctttgaag ataaatccaa cgacgtgatc caggatgacg tattccgtcg cctgtctgcc
     4561 tgccacaacg tgctgtttac cgggcaccag gcattcctga cagcagaagc tctgaccagt
     4621 atttctcaga ctacgctgca aaacttaagc aatctggaaa aaggcgaaac ctgcccgaac
     4681 gaactggttt aatcttgccg ctcccctgca ttccagggga gcttattcag ataatcccca
     4741 aagacctttc atcttctatt cttaaaatag ccctgagtca gaaactgtaa ttgagaacca
     4801 caatgaagaa agtagccgcg tttgttgcgc taagcctgct gatggcggga tgtgtaagta
     4861 atgacaaaat tgctgttacg ccagaacagc tacagcatca tcgctttgtg ctggaaagcg
     4921 taaacggtaa gcccgtgacc agcgataaaa atccgccaga aatcagcttt ggtgaaaaaa
     4981 tgatgatttc cggcagcatg tgtaaccgct ttagcggtga aggcaaactg tctaatggtg
     5041 aactgacagc caaagggctg gcaatgaccc gtatgatgtg cgctaacccg cagcttaatg
     5101 aactcgataa caccattagc gaaatgctga aagaaggtgc acaagtggat ctgaccgcga
     5161 accagttaac gctggcgacc gcgaaacaga cattaactta taagctggcg gatttaatga
     5221 attaatagct gccacagctc ccggcggcaa gtgactgttc gctacagcgt ttgccgttgg
     5281 gtaatgcaca catcccaatc gccgtaccat ccagttgacg ggcaacagaa agcgaaccgc
     5341 cgatcattgc acaatttgct tctccactac tggacatcga cgcttttaaa cctggcgcta
     5401 cgtgcgccgc agtcgcctgc tgaacaggtt cactactaca cgccgacaac aataaagcgg
     5461 cacaccctac ccaaaacgct gctcgcatct cttcttcctc tgatcttcaa gccaaacgac
     5521 accgccataa ataataggca gcacagaggg cggcgtcgag agctgtcctg cgcgttgccc
     5581 cgcattttta cttttttatg gctatttttt tgccctctgt ttgatcaaaa cattcattac
     5641 gctgatgtgg gggacacaaa agcgaaaatg cagaagaaag ccatttgcta aaattgaagg
     5701 attactactg ggcgcgcagc aatttcgtgc gcccctcatt tgcgcaatgt aagggtgtca
     5761 tatgattact attgatggta atggcgcggt tgcttcggtg gcgtttcgca ccagtgaagt
     5821 tatcgccatc taccctatta cccccagttc cacgatggca gaacaggctg atgcctgggc
     5881 cggaaacggc ttgaagaacg tttggggaga cacaccatgc gtggttgaaa tgcagtcgga
     5941 agcgggtgct atcgctaccg tgcatggcgc tttgcagacg ggtgcccttt caacatcgtt
     6001 tacgtcatcg cagggtttgc tgctgatgat cccgacactg tacaaactgg caggcgaact
     6061 cacaccgttt gtcctgcatg tagcggcacg taccgttgcc acacatgcac tctctatttt
     6121 tggcgatcat tccgacgtca tggcggtgcg ccagacgggt tgcgcgatgt tgtgtgcggc
     6181 aaacgtccag gaagcgcaag actttgctct catttcgcac atcgcgacgc tgaaaagccg
     6241 cgtgccattt attcatttct ttgatggttt ccgcacgtcc cacgaaatca acaaaattgt
     6301 cccgctggcc gatgacacga ttcttgatct catgccgcag gccgaaattg atgctcatcg
     6361 cgcccgggcg ctcaacccgg aacatccggt gatccgtggt acgtccgcca atcctgacac
     6421 ttatttccag tctcgcgaag ccaccaaccc ctggtacaac gcagtctatg accatgttga
     6481 acaggcgatg aatgatttct ctgccgcgac aggtcgtcag tatcagccgt ttgaatatta
     6541 cgggcatccg caagctgaac gggtgattat cctgatgggc tcagccattg gcacctgtga
     6601 agaagtggtt gatgaattgc taacccgtgg cgaaaaagtc ggcgtgctga aagttcgcct
     6661 gtaccgcccc ttctcagcca aacacttgct acaaactcta ccgggatccg tacgcagcgt
     6721 ggcggtactg gacagaacca aagaacccgg tgcccaggca gaaccgctct atctggatgt
     6781 gatgaccgca ctggcagaag cctttaataa tggcaagcgc gaaactctgc cccgtgtcat
     6841 tggtgggcgc tatggtcttt catccaaaga atttggcccg gactgtgtac tggcggtatt
     6901 tgccgagctc aacgcggcta aaccgaaagc gcgctttacg gttggtattt acgatgatgt
     6961 aaccaatctg tccctgccgt tgccggaaaa cacccagcca aactcggcga aacttgaagc
     7021 cttgttttat ggccttggta gtgatggcag cgtttccgcg accaaaaaca atatcaagat
     7081 tatcggtaat tccacgccgt ggtacgcaca gggctatttt gtttacgact ccaaaaaagc
     7141 gggcggcctg acggtttctc accttcgcgt gagcgaacag ccgattcgtt ccgcttatct
     7201 catttcccag gctgattttg ttggctgcca ccagttgcag tttatcgata aatatcagat
     7261 ggctgagcgt ttaaaacctg gcggcatttt cctgctcaac acgccgtaca gcgcagatga
     7321 agtgtggtcg cgcttgccgc aagaagttca ggccgtgtta aaccagaaaa aagcgcgctt
     7381 ctatgtgatt aacgcggcga aaatcgcccg caaatgtggc ctggcggccc gtattaatac
     7441 cgtcatgcag atggcttttt tccatctgac gcaaattctg cctggcgata gcgccctcgc
     7501 agaattgcag ggtgcgattg ccaaaagtta cagtagcaaa ggccaggatc tggtggaacg
     7561 caactggcag gctctggcgc tggcgcgtga atccgtagaa gaagttccgt tgcaaccggt
     7621 aaatccgcac agcgccaatc gaccgccagt ggtttccgat gccgcccctg atttcgtgaa
     7681 aaccgtaacc gcagcgatgc tcgcagggct tggtgatgcc ctccccgttt cggcgctgcc
     7741 gccagacggc acctggccga tgggcaccac tcgctgggaa aaacgcaata tcgccgaaga
     7801 gatccccatc tggaaagagg aactctgtac ccaatgtaac cactgcgtcg cagcttgccc
     7861 acactcagct attcgcgcaa aagtggtacc acccgaagcg atggaaaacg cccctgccag
     7921 cctgcattcg ctggatgtga aatcgcgtga tatgcgcggg cagaaatatg tcttgcaggt
     7981 ggctccggaa gattgcaccg gttgtaacct gtgcgtcgaa gtttgcccgg cgaaagaccg
     8041 tcagaatcca gagattaaag ccatcaatat gatgtcgcgc ctggaacatg tcgaagaaga
     8101 gaaaatcaat tacgatttct tcctcaacct gccagaaatc gaccgtagca aactggaacg
     8161 tattgatatt cgtacatcgc agctgattac accgctgttt gaatattccg gtgcttgctc
     8221 cggttgtggc gagacgccgt atattaaatt gctgactcag ctctatggcg accggatgtt
     8281 gatcgctaac gccaccggct gttcttcaat ttatggcggt aacctgccct ctacaccgta
     8341 taccaccgat gccaacggtc gtgggccggc atgggcgaac tctctatttg aagataatgc
     8401 cgaatttggc cttggtttcc gcctgacggt cgatcaacac cgtgtccgcg tgctgcgtct
     8461 gctggatcaa tttgccgata aaatcccggc ggaattactg acggcgttga aatcagacgc
     8521 tacgccagag gttcgtcgag aacaggtcgc agctttacgc cagcaactca acgatgttgc
     8581 cgaagcacat gaactgctac gtgatgcaga tgcactggtg gaaaaatcaa tctggctgat
     8641 tggtggtgat ggctgggctt acgatatcgg ctttggcggt ctggatcatg tattgagttt
     8701 gacggaaaac gtcaacattc tggtgctgga tacgcaatgc tattccaaca ccggtggtca
     8761 ggcgtcgaaa gcgacaccgc tgggtgcagt aactaaattt ggcgagcacg gcaaacgtaa
     8821 agcgcgtaaa gatcttggcg tcagtatgat gatgtacggt catgtttatg tggcgcagat
     8881 ttctctcggc gcgcagctga accagacggt gaaagcgatt caggaagcgg aagcgtatcc
     8941 gggaccatcg ctgatcattg cttatagccc gtgtgaagag catggttacg atctggcact
     9001 cagccatgac cagatgcgcc aactcacagc taccggcttc tggccgctat atcgcttcga
     9061 tcctcgtcgt gccgatgaag gcaaactgcc gctggcgctg gattcacgcc cgccgtcaga
     9121 agcactggaa gaaacgttac ttcacgagca acgtttccgt cgtctgaatt cgcagcagcc
     9181 agaagtggca gaacagttat ggaaagatgc tgcagctgat ttgcaaaaac gctatgactt
     9241 cctggcacaa atggccggaa aagcggaaaa aagcaacacc gattaaatgc tctggataag
     9301 gattacccaa ttctaaaaaa aagcccggac gactgttcgg gcttgtcttt ttatatgttg
     9361 gaaaatcagt ggcaatgcaa tggcccaaca gaaaataaaa tctgtgaca
//
LOCUS       DAZPAC010000157         9397 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZPAC010000157 DAZPAC010000000
VERSION     DAZPAC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.157"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            197..301
                     /locus_tag="ACHUPM_003163"
     CDS             197..301
                     /locus_tag="ACHUPM_003163"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR0870865.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(350..1603)
                     /locus_tag="ACHUPM_003164"
     CDS             complement(350..1603)
                     /locus_tag="ACHUPM_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0870866.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="ACHUPM_003165"
     CDS             1710..2603
                     /locus_tag="ACHUPM_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0870867.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="ACHUPM_003166"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="ACHUPM_003166"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR0870868.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="ACHUPM_003167"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="ACHUPM_003167"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0870869.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHUPM_003168"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHUPM_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR0870870.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHUPM_003169"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHUPM_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR0870871.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHUPM_003170"
     CDS             complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHUPM_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR0870872.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHUPM_003171"
     CDS             complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHUPM_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR0870873.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..>9397)
                     /locus_tag="ACHUPM_003172"
     CDS             complement(8323..>9397)
                     /locus_tag="ACHUPM_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFR0870874.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2449 a         2379 c         2438 g         2131 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccgcaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgg
//
LOCUS       DAZPAC010000158         9212 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZPAC010000158 DAZPAC010000000
VERSION     DAZPAC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.158"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            73..732
                     /locus_tag="ACHUPM_003173"
     CDS             73..732
                     /locus_tag="ACHUPM_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFR0870875.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="ACHUPM_003174"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="ACHUPM_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR0870876.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="ACHUPM_003175"
     CDS             complement(1860..2570)
                     /locus_tag="ACHUPM_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR0870877.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="ACHUPM_003176"
     CDS             3004..3294
                     /locus_tag="ACHUPM_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFR0870878.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="ACHUPM_003177"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="ACHUPM_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR0870879.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            3927..3998
                     /locus_tag="ACHUPM_003178"
     CDS             3927..3998
                     /locus_tag="ACHUPM_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870880.1"
                     /translation="MRNAVRKAGITSRQRLLLFQIAG"
     gene            complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="ACHUPM_003179"
     CDS             complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="ACHUPM_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR0870881.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="ACHUPM_003180"
     CDS             complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="ACHUPM_003180"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR0870882.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="ACHUPM_003181"
     CDS             complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="ACHUPM_003181"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR0870883.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7538..7837)
                     /locus_tag="ACHUPM_003182"
     CDS             complement(7538..7837)
                     /locus_tag="ACHUPM_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR0870884.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8012..9007
                     /gene="wecH"
                     /locus_tag="ACHUPM_003183"
     CDS             8012..9007
                     /gene="wecH"
                     /locus_tag="ACHUPM_003183"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR0870885.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9049..>9212)
                     /locus_tag="ACHUPM_003184"
     CDS             complement(9049..>9212)
                     /locus_tag="ACHUPM_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR0870886.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         2286 a         2328 c         2268 g         2330 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagctgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atccacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gcacagaatc taagatccct gccatttggc ggggattttt
     3841 ttatttgttt ttcaggaaat aaataatcga tcgcgtaata aaatttagta ttatttttgt
     3901 gaagaataaa tttgggtgca atgagaatgc gcaacgccgt aagaaaggcg ggaataactt
     3961 cccgccaaag actcttactc tttcaaattg caggctaaaa atgccgccag ctcataactc
     4021 tcctgtttaa tatgcaattc acacagtgaa tctcttatca tccaggtgaa aaataaaagc
     4081 gtgaaacaaa tcactattaa agaaagtaat ctatatttct gcggcattcc cagcctcctg
     4141 tgttgatttc caacgagtaa gaggctaacc ttatggtgtt caggcatagg gtagcctcac
     4201 tttgatttat agtcaggtgg ggcttttctc tgtctgcctt tcggtgaata cctgagacaa
     4261 acagtctcaa gcacccgtgg ctattctagc ttaataagtt tgtttcttct ccttgatata
     4321 atcctaaaaa aacctcataa aattaatata tgagataatc tttattcaac agaagattat
     4381 taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta
     4441 ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag
     4501 tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac
     4561 tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg
     4621 gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc
     4681 cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc
     4741 gtcagatttc gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc
     4801 atccagggta cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt
     4861 cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca
     4921 ggcgcggaag cgaccgagca taaagtcgat aacatcatca actacgttgg cgttagtcag
     4981 cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt
     5041 cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt
     5101 gtcgcctttc ggatgctgac cgataccgaa gatacccgcc agagtatcca tcttatcagc
     5161 aatcgccagc gcacaagcta ccgggttgga cggcaggtca tcaccggcaa agcgcggctg
     5221 atactgctca ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg
     5281 catccccata acgccttggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca
     5341 cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat
     5401 ccagccagcc agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg
     5461 gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt
     5521 gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat
     5581 ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc
     5641 atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc
     5701 agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac
     5761 cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg
     5821 cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac
     5881 tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg
     5941 ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatggttgc
     6001 cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac
     6061 gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagcg aagtcgcaac
     6121 catattcggc agtagtgctt cagtgctttc gcccttcaca tgggcgcgat acagcagcca
     6181 ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
     6241 ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg
     6301 gccgcgtttt tcgatttcac gatctggttg cgcttccgcc aggttagcta ctttcaacgc
     6361 cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag
     6421 ctccgcagta aagttcgcag caaaggactc agccaggctg cgcagtgctt ttggtggcag
     6481 ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc
     6541 tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc
     6601 tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcagtgacgg agatggcttt
     6661 acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag
     6721 cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact gctcgaagca
     6781 ggtgaacagg aaatccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt
     6841 ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agaccaggtc
     6901 gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc
     6961 gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga actgcgtcac
     7021 ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag tcgggttttc
     7081 ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca gctctttcag
     7141 agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg
     7201 atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga
     7261 aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg
     7321 gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg
     7381 agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg
     7441 catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga
     7501 tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa
     7561 aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta taaccgcgca
     7621 tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt
     7681 tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat
     7741 caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaccaaccc actagcacca
     7801 taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg
     7861 tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct
     7921 ataagctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga tttatcggtc
     7981 agactttttt taaccagata cagagacaca gatgcagccc aaaatttact ggattgataa
     8041 cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct ggtatgtgac
     8101 caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga attctgcctc
     8161 tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg gcgaacgcag
     8221 cgcccagccg cgccatttct tgcgtatcgg cttatgtctg attttttata gcgcaatcgc
     8281 actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga aaaacctgct
     8341 gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga tttatctggt
     8401 ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc taatggcggt
     8461 gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt ttgaatggct
     8521 gccaattaac ttatatatca atggcgatac tttttactac attctgtatg gcatgttggg
     8581 ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga gcgccgcgct
     8641 gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc agtggcgcgg
     8701 aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct gcgcaatcgc
     8761 gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac ttggcttaat
     8821 ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg cgctgcgcac
     8881 ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct tttgcgcgac
     8941 gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa acagattagt
     9001 gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt attcgctgta
     9061 ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggcatcac gaatattctt
     9121 ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa aacctttttc
     9181 actgagtaat aatgtcgcat tccacagacc ta
//
LOCUS       DAZPAC010000159         9185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZPAC010000159 DAZPAC010000000
VERSION     DAZPAC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.159"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="ACHUPM_003185"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="ACHUPM_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="ACHUPM_003186"
     CDS             complement(1751..2509)
                     /locus_tag="ACHUPM_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR0870887.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHUPM_003187"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHUPM_003187"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR0870888.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHUPM_003188"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHUPM_003188"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR0870889.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="ACHUPM_003189"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="ACHUPM_003189"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR0870890.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="ACHUPM_003190"
     CDS             complement(5474..6079)
                     /locus_tag="ACHUPM_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870891.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..6849)
                     /locus_tag="ACHUPM_003191"
     CDS             complement(6082..6849)
                     /locus_tag="ACHUPM_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870892.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6852..7562)
                     /locus_tag="ACHUPM_003192"
     CDS             complement(6852..7562)
                     /locus_tag="ACHUPM_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870893.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7578..9083)
                     /locus_tag="ACHUPM_003193"
     CDS             complement(7578..9083)
                     /locus_tag="ACHUPM_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR0870894.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2372 a         2358 c         2260 g         2195 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgctagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagtacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgta aaattgcccg ttcatccgca cgccagtttt ctttgttggc
     6541 aaggttgata cggcgcagag aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac
     6601 aaattccagt aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc
     6661 tgggctacta ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtactccg
     6721 taatgccttt tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg
     6781 cacccgataa cgacgaaagg tgtgataaat ctgctcaatt aatgcctgca tggcggggct
     6841 gggttgcata gttaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga
     6901 gcattcgccg cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg
     6961 caactattgc agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg
     7021 tagagtttca gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat
     7081 ttccaggcat ctttaaagac ctgaaagata tagacatcct caatccagga gtcacatttt
     7141 ttaagcgggc gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt
     7201 acggtgacgt catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag
     7261 gggtcaaatc caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg
     7321 gaaaatcccg gccatacctt gctggaggtt aagccaatat ttaatgtgac atcatatcga
     7381 agcaatccct caagaccgtc agcaccgacg aacttttccc cttcatccaa ggcctggtta
     7441 ataagttgct gaacaatgcc ttcatcccag tatgccgatt tgcagttggc aatcgtaata
     7501 tccacgcctg tgaagggatc agtaccggca aggcttgcaa ataatgtatt aactaattcc
     7561 atttttatta tccagcttta aggtagcgtc ttatacatcg ggacataacc tacatcttcc
     7621 accaaactct gtccctgcgg cgttaaaaac cactccaaca atttttgtgt ctctgacgtc
     7681 gtattttccc gcgttaccat aaaggcatcg acgatatagg gatatttgcc gttgcgaatg
     7741 ttttcggcag tcggtgcaat accgttaatc gctagcagtt ttatattttt atcggcgttc
     7801 atttgtgttg cgtaataacg gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg
     7861 gcaacgactt taatcattcc ctccatcata cttgccactt ctgtttcctg cggcgagatc
     7921 atgcggacat ttttcatgac ctgggattgc atcaccgtct ggctaccaga gtcttccggg
     7981 cgttgccagg tctggatctc ctgatcgtta ccgccaacag tacgccagtt ggtaattgca
     8041 ccactgaaga tgtcacgcac ctgttgttca gtcagggaat taaccggatt atccgcattg
     8101 acgatgaaaa caaacgcttc acgggcaaaa ggcgtgtaca gcaaagtaac gcccgattcc
     8161 tcagcgcgtt ttttctgccc accggaaggt tgcgccacaa aaataatatc agcatcacct
     8221 ttaacaatcc tgttatacgc atcgggggta cgagagctct ccagatattc cctggtgtga
     8281 aaatcttccg gtataacgct taaagcataa aatgcagagg cataaattgg gtacgccgcc
     8341 gtagcaccat cgagtcgcgg ccagttttgc gtaaactgaa tttgcggttt accacgcagt
     8401 ggtgtcagtt gattattgag tttatcgggt cgccaggccc aggtatcaat attttcgcgc
     8461 acaccgacac cgggattgag ctgtttatat aaataaccct gataaccagc gacaaaacca
     8521 agcatgagaa ggataaacag gagggaatta cggcaacgta gcccggatgt accggttaaa
     8581 ggatgtctgg ttggccggcc gtaccccagc gaaaaactga tatgcgcgcc aacgggtata
     8641 atgactatcc accagtactc acctccgact actgagacta acaaaacgcc cagccagggg
     8701 agcataagat cagccaacca acccgatccc atctggtgaa attgataatc tgccagatgc
     8761 agcgcgatta tccagccaac caggattaat aaaccgggta ctaaaaaaag acattgtcgc
     8821 cagacggcta tttctctttt ttctggtgca ctatcgcctg cccatactcg ccccgagaaa
     8881 aatagcatta atgcgcccag cgccagcata aaaatgttga gcagaaggta acttgttggc
     8941 gtgataagga tattacaaac ttcaataata ccggcaagtc cgacacccag catagcaaca
     9001 accgccgcca aaaattgcgt cagtatgggg atgccgaaaa aagtcattac cagcgaggac
     9061 aaaatccatt ttctgttttg cattattctt tccattcttt ttgaatggtg aaattatact
     9121 ccccgagtcc ccttgcccct tctggacact tttccgaaat gatggcggaa aaaaacggga
     9181 ccctt
//
LOCUS       DAZPAC010000160         9097 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZPAC010000160 DAZPAC010000000
VERSION     DAZPAC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9097
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.160"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="ACHUPM_003194"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="ACHUPM_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR0870895.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHUPM_003195"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHUPM_003195"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR0870896.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="ACHUPM_003196"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="ACHUPM_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHUPM_003197"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHUPM_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR0870897.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHUPM_003198"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHUPM_003198"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR0870898.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="ACHUPM_003199"
     CDS             complement(6490..6846)
                     /locus_tag="ACHUPM_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0870899.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="ACHUPM_003200"
     CDS             complement(6850..7266)
                     /locus_tag="ACHUPM_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR0870900.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHUPM_003201"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHUPM_003201"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR0870901.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8492..8714
                     /locus_tag="ACHUPM_003202"
                     /pseudo
     CDS             8492..8714
                     /locus_tag="ACHUPM_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2369 a         2212 c         2189 g         2327 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgcc cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aaccccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     8281 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     8341 tttgaaagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     8401 acaatcgaag tttataaaga tgattcctga ttgaccagaa ccctatccta caatggtgac
     8461 taattttatt actcccgatg gagatgatga tatgaatata tcatatgtga atagcaacaa
     8521 aacgacatct ttacctgttg agctagatgc actaaataat aaagacatct cttatgccaa
     8581 agatttttct tatgctaaag atttcttttt atacatagaa acacagttaa aaattgcaaa
     8641 agatttttgt agacctggag aagaagtatc aagttctatt gcaagtaaag tttttcacgc
     8701 atttatcgat ttagttaaca aaatcagggg taagaaagac tttatgtata tttgcacgct
     8761 ttgctgtttt gccgaggagg ttaaaggtga ttattctcat tacaggacct ttttatttga
     8821 tattggtaat caatataagg ttaaacttac acaaagcgga aaaaaagagt tctctttaac
     8881 attagaattt aacgatacta taattgaatc tcagaaagtc acaggcaata aggcaaagta
     8941 tattcttgag gatatagaaa tattctatcg taacaaaccc gatacttatt attaaattta
     9001 ataatggtta gatgtgcaga aattattact ccacacgaaa aaatagggaa aggaattcca
     9061 gataattggg ataaacttaa caataaaaga actgaca
//
LOCUS       DAZPAC010000161         9084 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZPAC010000161 DAZPAC010000000
VERSION     DAZPAC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.161"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..103)
                     /locus_tag="ACHUPM_003203"
     CDS             complement(<1..103)
                     /locus_tag="ACHUPM_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870902.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTA"
     gene            complement(254..2125)
                     /gene="ppiD"
                     /locus_tag="ACHUPM_003204"
     CDS             complement(254..2125)
                     /gene="ppiD"
                     /locus_tag="ACHUPM_003204"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0870903.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2317..2607)
                     /gene="hupB"
                     /locus_tag="ACHUPM_003205"
     CDS             complement(2317..2607)
                     /gene="hupB"
                     /locus_tag="ACHUPM_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR0870904.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2798..5152)
                     /gene="lon"
                     /locus_tag="ACHUPM_003206"
     CDS             complement(2798..5152)
                     /gene="lon"
                     /locus_tag="ACHUPM_003206"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR0870905.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5340..6614)
                     /gene="clpX"
                     /locus_tag="ACHUPM_003207"
     CDS             complement(5340..6614)
                     /gene="clpX"
                     /locus_tag="ACHUPM_003207"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR0870906.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6740..7363)
                     /gene="clpP"
                     /locus_tag="ACHUPM_003208"
     CDS             complement(6740..7363)
                     /gene="clpP"
                     /locus_tag="ACHUPM_003208"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR0870907.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7609..8907)
                     /gene="tig"
                     /locus_tag="ACHUPM_003209"
     CDS             complement(7609..8907)
                     /gene="tig"
                     /locus_tag="ACHUPM_003209"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR0870908.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
BASE COUNT         2011 a         2513 c         2136 g         2424 t
ORIGIN      
        1 ccgccgtcgg tttcgccaca gaagctgccg ccagcgcgct atgagacatt ccggcacagg
       61 ccagggacag ggtaatgagc agtgctttaa ttccgtgttt catactgttt tctccttgtt
      121 tgttaacagt ggaatcacct taacgggagt gaaaacagta aacaaatggc aatgttcaga
      181 aatggaaaag gccgcgaaag cgaccttttc ttgttacagc tgcaacgcat ttttttgcga
      241 ggcggcttca ggattattgc tgttccagcg catcgccaat tttgattttc gcctctttac
      301 gcaggttact catcagagct tcaaagacga tttgtgcgtt gttctgggtg ataccctgca
      361 ccatcgcttt tttctgatct tccggcattg aaccttgttt cacttcatcc agcgccagca
      421 gaaccacatt accttgcata tcggtcgcca taccgtagct cggtttgtct ttcgctggca
      481 gtggcagtgc aaacgccgcc tggctaatcg ggtcacgacc ggaacggctt aaggttttcg
      541 gctcgccaaa tttcagaccg gcagcctgca tagcttccgc ccctttgccg gctttcaaat
      601 caaccagcag tttctcagca tccactttcg cctgttgttc agctttgttg tgctgaacca
      661 gtgccttaac ttgttcctga acatctgcca acggtttcac cgcttccggt ttgtgctcgc
      721 tgatgcgcag cacgaatgcg cggtcgccgt ctacggtgat gatgtcagag ttgatgcccg
      781 gcgcgccgtt ttcacctacc agaccgccgt taaagatagc gtcggcaacc ggcttgaagt
      841 tcaactcttc cggcaggtta tctttgctga accaacccgt ctgagtggct ttaacgccgg
      901 cagcttgctc tgcaccggcc agagactcgg tgtcgttgct tgccgcatcg ctcactttct
      961 gctgcagcgc atagtacgca tcgagggctt tttcgtgttt cactttcgcc gcaatgtcgt
     1021 cacgtacttc gtctaacgat ttcactttcg ctggctgaat gtcgtccaga cgtacaatca
     1081 ggaaaccgac cgaagatttg atgacaccag acagttggcc tttttctttc agaccagcat
     1141 ttttcagttc gtcggggata gtggcatctt ctaaccaacc catatcgccg ccgttacggg
     1201 cagagataat atcagcagat ttttctttgg ctaatgcagc aaaatcaccg cctttattca
     1261 gctcatcaag taccgctttc gcttcatctt cagttttggt ctggatgatg ctgtagcggg
     1321 tacgctgcgg ctgggtgaat tgatcctgat gctgatcgta gtagctctgg atatccgcat
     1381 cgctaaccgg ttgctgcatc gttgcggcat ccagcttgat gtaactcacg cggaattgtt
     1441 ccggcgtcat gaaattgttt ttgttttgtt cgtagtagct ggcaatctcc tgttcggtca
     1501 caggctgctt cgccgccagc gcgttaacat cgatagtcgc ctcacgcacc acgcgttgtt
     1561 gcgcgaccag tgccgccagc tcgtcggttt cacctttcag cataaaatcg gtaccggcaa
     1621 caccgttaat cagctgttgg gtagtgagct ggttacgcag cgcctgggcg tactgatcgg
     1681 cggtcatccc catctggttg aggataccgt tatagcggct gttatcaaat ttgccgtcaa
     1741 cctggaaggc tggggtcgcg aaaatcgcct gtttaacctg ctcatcactg atacccagtt
     1801 tcagctcacg agcgtactga tccaacagcg cctcgtcgat cagacgattc agcacctgtt
     1861 gacgcagggt tttcatatag ccttcgttcg ctgccagctc ggagtattga tcgcccagct
     1921 gttgctgcat gcgattacgc tcgctgttaa aggcgttctc gaactgccca cggctgattt
     1981 cctggtcatt cacttttgcg gcgtagttat tgcctccgcc aatcaggtaa ccactcacgc
     2041 cggtcaatat gaacgacacg ataatgatac cgaaaataat cttgagcacg agactgtttg
     2101 cagccgtgcg taagctgtcc atcatggtgt aacaacactc cgctgtagat gactgtgtac
     2161 ctcacgcaac gtatcgatgc gcgcaaaggc ctattgtgac aagaaacggg ggcaattgtc
     2221 agcccacaag acacagaaag tcaccgaata caaataaaaa aggcacatca gtagatgcgc
     2281 ccttgaactt cgtcacatcc ccactgggga caacgcttag tttaccgcgt ctttcagtgc
     2341 tttacctgca cggaagctcg gtactttagc ggcagcgatg gtgatctctt taccggtctg
     2401 cgggttgcgg ccagtacggg cagcacgctc tttaacggca aaagtaccaa aacctaccag
     2461 tgctacatca tccccttctt tcagagattc agttacggaa gcaataatag catctaacgc
     2521 acggccagcc gcagctttag agatatcagc ccctgcagca atcttgtcga tcaattgaga
     2581 tttattcact cttctcttcc tctttataat ttatatcgca cttgaatcct tcaaggtacg
     2641 aacgcgcagc agttatatca ggccagccat ccccttacaa caccccttaa taagggcaag
     2701 cccgatccgc catctaactt agcgagacaa aaaaaggctg gcaagcccga attagcctgc
     2761 cagccctgtt tttattagtg cattttgcgc gaggtcacta ttttgcagtc acaacctgca
     2821 tgccagacgg ttcattttgc agcgccagag tcagaacttc ctcaatgcgc ttcacaggat
     2881 gaatgtccag atcggcaatt acgttgtcag gaatctcttc cagatcgcgt ttattttcga
     2941 acggaattag cactgtttta atcccgccgc gatgcgctgc caggagtttt tctttcaaac
     3001 caccgatcgg cagtacctga ccacgcagag tgatctcacc ggtcattgcc acatcggcac
     3061 gaaccgggtt accggtcagg caagaaacca gcgcggtgca catagcaata ccggcactcg
     3121 gaccatcttt cggcgtcgca ccttccggta cgtggacgtg gatgtcacgt ttttcgtaaa
     3181 aatcagggtt gatccccagt ttttccgcac gcgcacgaac caccgttaac gccgcctgaa
     3241 tagactcctg catcacttcg ccgagcgaac cggtataggt cagtttgcct ttacccggaa
     3301 cacatgcggt ttcaatggtc agcaagtcac cgcccacttc cgtccacgcc agaccggtta
     3361 cctgaccgac acggttttcg ttatcagcgc gaccatagtc gaaacgctga acaccgaggt
     3421 agtcatgcag gttatcgccg ttaatttcga tatgttttaa tgacttatcg agcagtaact
     3481 gcttaaccgc tttgcggcac agtttggaga tttcacgctc cagaccacgc acgcccgcct
     3541 cacgggtgta gtaacgaata atgccgataa tggcgctatc gtcgacggtc agctcacctt
     3601 ttttcagtgc attacgttca atctgcttcg gcagcaggtg acgtttggcg atgttcagtt
     3661 tttcatcttc ggtataaccg gagaggcgaa tcacttccat acgatccagc agcggtgccg
     3721 gaatgttcat ggagttcgac gtcgcgacaa acatcacgtc gctgagatcg taatccactt
     3781 ccaggtagtg gtcgctgaac gctacgttct gctctggatc cagcacttca agcagtgcag
     3841 aggccggatc gcctcgcatg tcagaagaca ttttgtcgat ctcatcgagc aggaacagcg
     3901 ggtttttcac gcccactttc gccattttct ggatcagttt acccggcata gaaccgatgt
     3961 aagtacggcg gtgaccacgg atttccgctt catcacgcac gccgcccagc gccatacgga
     4021 catatttacg cccggtggct ttggcaatgg actgaccaag agaggtttta cctacccccg
     4081 gcggccctac caggcagagg atcggtccct tgattttgtt gacacggctt tgaaccgcaa
     4141 ggtactcaag gatgcgatct ttcacgcgct ccagaccata atggtcggta tcaaggattt
     4201 cctgcgcctg acgcaggtct tttttgacct tgctgcgcgc attccacggt acctgtacca
     4261 tccagtcgat ataaccacgc actacggtcg cttctgccga catcggagac atcattttca
     4321 gcttctgcaa ctctgcttcc gctttctctt ttgcctcttt cggcatcttc gccgcgtcga
     4381 ttttgcgctt cagggcttcg ttttcgtccg gcgcgtcgtc catttcaccg agttctttct
     4441 gaatagcttt catttgctcg ttcagatagt actcacgctg ggatttctcc atctgctttt
     4501 taacgcggtt gcgaatgcgt ttctcaacct gcagcagatc gatttccgat tccatcattg
     4561 ccatcagata ttccagacgt tcgttaacgt cggacatctc cagaacggac tgtttgtcag
     4621 ccagtttcag cggcatatgt gcagcaatgg tatccgccag acgcgccgga tcgtcgatgc
     4681 tattcagcga cgtcagcact tctggtggga tttttttgtt cagcttgatg tagccttcga
     4741 actggctgat tgcagtacgc accagcactt cctgttcccg ctcatcaatg gtcggcgact
     4801 ccagatactc cgccttcgca gaaaagtgtt cgccattgtc agagagcgca gaaatacgcg
     4861 cgcgctgtaa cccctcgacc agcactttga cggtgccgtc aggcagtttc agcatctgca
     4921 atatagaggc cacggtcccg acggtgaaaa gatcgtttac acccggctca tccgttgaag
     4981 cttctttctg cgcgaccagc ataatttttt tatcatggtc catcgccgct tccagacaac
     5041 ggatagattt ttcccgcccg acaaataagg ggatgaccat gtgcggataa accaccacat
     5101 cgcgcagcgg caatacgggg atttcaatgc gttcagaacg ctcaggattc atagagctct
     5161 ctcttagttt aatttccgcc aggtaatcag atgacacgac tgtgcttcac gccatctatt
     5221 aacatgtacg tcagtatatg gggatgtttc ccccacattc aacgccgaga atagaggaaa
     5281 aattaaaggg gagataaaat cccccctttt tggttaacta attgtatggg aatggttaat
     5341 tattcaccag atgcctgttg cgcttccggc ttaccataaa tcagcaacgg tttgctttga
     5401 ccatcaatta ccgactcgtc gataaccact ttttcgacgt cttccatgga cggcagatcg
     5461 tacatggtat cgagcagtgc ggcttctacg atggaacgca ggccacgggc accggtttta
     5521 cgcgccatcg ctttcttagc gatagcatcc agcgcctcgt cacggaattc cagatccacg
     5581 ccttccagat taaacagcgc ctgatactgc ttggtcaggg cgtttttcgg ctctttgagg
     5641 atctgaatca gagcttcttc gctcagttca ttcaacgttg ctacaaccgg cagacgacca
     5701 atgaactcag ggataagacc aaacttgatc agatcttccg gttcaacctg cgccagcagc
     5761 tcgccttcgc ttgctttgtc ggacttcgct tttaccgtcg cgccaaaacc aatgccggag
     5821 ccggtttcta cacggtggga aatcacttta tccagaccgg caaacgcacc gccacaaata
     5881 aacaggatct tagaggtatc aacctgcaag aattcctgct gcggatgttt acgcccacct
     5941 tgcggtggaa cagcagctac cgtaccttcg atcagtttca acagtgcctg ctgtacgcct
     6001 tcaccggaaa cgtctcgggt aatggacggg ttgtctgact tacgagaaat cttgtcgatt
     6061 tcatcgatgt agacaatccc gcgctgcgct ttctgtacgt cgtaatcgca cttttgcaac
     6121 agcttctgaa tgatgttttc aacgtcttcg cccacataac cggcttcggt cagcgtggtt
     6181 gcgtcggcca tggtgaacgg gacgtccagc aggcgcgcca gcgtttcagc cagcagcgtt
     6241 ttaccggaac cggtcggacc gatcagcaga atgttacttt tgcccaactc gacaccattg
     6301 ctggtatcgc cgttgcgcag acgtttgtaa tggttgtata ccgcgaccgc cagcactttt
     6361 ttcgcctgtt cctggccgat aacgtaatcg tccaggtggt tgcgaatttc atgcggcgtc
     6421 ggtagcgcac tgcgttcacg atgcggtgca acttctttaa tctcttcgcg aatgatgtcg
     6481 ttacataaat caacacattc gtcgcagata tacacggatg gaccggcaat cagcttgcgc
     6541 acttcatgct ggcttttgcc gcaaaaagag caatacagca atttgcctga gccatctttg
     6601 cgtttatctg tcatgggtca aaacctcttc tttgttcttt gtgccgcaca cgacgacgca
     6661 aatgccattc tcaggcgcaa gccgcttaca gcgttgtgcc gccctggata agtatagcgg
     6721 cacagttgcg cctctggcat caattacgat gggtcagaat cgaatcgacc agaccgtatt
     6781 ccaccgcttc aggggcggaa aggaagcgat cgcgctcggt atcacgttca atctgttcta
     6841 atgattgacc cgtatgaagc gccataagtt cattcatgcg ccctttaact ttcagaattt
     6901 cacgggcatg aatttcgata tcggtcgcct ggccctggta gccgcccaac ggttggtgaa
     6961 tcatcacgcg cgaattcggc aggcaaaaac gtttaccttt tgcccctgcg gtcagcaaga
     7021 aagcgcccat cgaggccgcc tggcccatac agatggtgct gacatcaggc ttgataaact
     7081 gcatggtgtc atagatagac atcccggcag tgatcacccc gcctggggag ttaatgtaca
     7141 gatagatatc tttttctggg ttttccgctt ctaggaacag catctgcgcc acaatcaggt
     7201 tcgccatgtg gtcttcaacc tggccagtca gaaaaatgac gcgttcctta agtagacgag
     7261 aatagatatc aaaagagcgc tcaccgcgtg aggtctgttc aatgaccatc ggcaccagcg
     7321 ccatatgggg tgcaaagtta tctcgttcgc cgctgtatga catttccgtc tcctggataa
     7381 aattgaaaaa acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg
     7441 tgttatgggt acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca
     7501 ccgaaagagg cttttcgcac ggttccatgc aaaattcatg acaaaaaaaa gcccaccacc
     7561 tggcggtgac gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg
     7621 gttcatcagc tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc
     7681 ttcaacagcc tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt
     7741 gctgtagaac tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag
     7801 gcctttcacg cgctcttcgt cagctttcag ttcgttggtg cggataactt cgcccagcag
     7861 caggccaaca actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag
     7921 agcttgtttt tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc
     7981 gctgtcgatc agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc
     8041 ctgagactta acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac
     8101 ttcagcgcgc agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc
     8161 agtcagttcc ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc
     8221 tttacctttc agattttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc
     8281 ttcgccagct ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc
     8341 catcgccagt acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc
     8401 ggtgaagtcg atggttacgc ggtcttctgc ttcaacggcg ccgtcttttt ctttccaggt
     8461 cgcctgctgt ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac
     8521 gatcggtttt tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc
     8581 aaactctaca gagtaggtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg
     8641 tgcgccagcc ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag
     8701 gtcacccaga acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg
     8761 cactttgcct ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct
     8821 tttaacagcg gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc
     8881 ttgagtggtt tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca
     8941 actcttaccc cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa
     9001 tcacattccc tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc
     9061 ggcatcactt ttatgagtcg agaa
//
LOCUS       DAZPAC010000162         9075 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZPAC010000162 DAZPAC010000000
VERSION     DAZPAC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.162"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(506..1273)
                     /gene="fecE"
                     /locus_tag="ACHUPM_003210"
     CDS             complement(506..1273)
                     /gene="fecE"
                     /locus_tag="ACHUPM_003210"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR0870909.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="ACHUPM_003211"
     CDS             complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="ACHUPM_003211"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR0870910.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="ACHUPM_003212"
     CDS             complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="ACHUPM_003212"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR0870911.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="ACHUPM_003213"
     CDS             complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="ACHUPM_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR0870912.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="ACHUPM_003214"
     CDS             complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="ACHUPM_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR0870913.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="ACHUPM_003215"
     CDS             complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="ACHUPM_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR0870914.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="ACHUPM_003216"
     CDS             complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="ACHUPM_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR0870915.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8188..8256
                     /gene="ykiE"
                     /locus_tag="ACHUPM_003217"
     CDS             8188..8256
                     /gene="ykiE"
                     /locus_tag="ACHUPM_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFR0870916.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            8343..9040
                     /locus_tag="ACHUPM_003218"
                     /pseudo
     CDS             8343..9040
                     /locus_tag="ACHUPM_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
BASE COUNT         1988 a         2509 c         2585 g         1993 t
ORIGIN      
        1 cactttttag aaaaatcgat ggtcagaaac tggattagca attccgttcc agggttgctt
       61 ttgatttacg ttggcgtctg atcattgatt tatcctcaaa agcccaacct cattggtaat
      121 gaaccagctc cgtgaatgtc cgctctggca cagagcgaaa ttttttgatc tcccccccct
      181 gaaatctaaa cttagtcatg tcacgttttt gggtttctaa aattttaact tcgcgttttt
      241 cgttgccgta agggttatac agaaatgtcc gttaagcaga gttcaaaatt gattgccgtg
      301 atcacgactg gtttgaaagc cgcgcccaag cctgtacagc tctggtttgc gttgattatg
      361 aacctgtcag cctaaagcaa gcggatggac gatgagtatt ggtaatcttt cagagtccgg
      421 aaaagttcag ccccagtctg aacaggcttg ctggcgccag tccagtttca ttcagtcgtg
      481 gtttggttct tacggcctgt gcaatctacc tcattaggca catcggcctg ccagataccg
      541 gctcggggtg tatttccgct tccacgctga atactgttct cagcaatcct ggggtcatca
      601 cctcttctgg tgtgccttgc gccataacat gtccgtttgc cattaccacc agttgatcgc
      661 agtaccggct agcctgatta aggtcgtgca gcacagcgac caccgttttc ccctgagtcc
      721 ggagttcgcc catcaaccgc atcaggtcca cctggtgatt gatatcaaga taggtggttg
      781 gctcatcaag taatacaacg ggcgtattct gggccaggac cattgccaga aatgcgcgct
      841 ggcgctgacc gccggaaagc tcggttaacc gacgaacggc aagatgattg atccgggtct
      901 ggttcatggc gacattaact cgtgcattgt cttcagcgga gagacgcccc cagagtgaca
      961 gccagggatt acgaccatac gaaaccagct cctggactgt gatcccctct ggcgttaaat
     1021 ggtgctgagg cagcagcgaa agcctgcggg ccaactggcg cgatgagagc atatttatgg
     1081 gattatcgcc gagaaatacg gtgccagact gcggcattaa aagccgcgaa aaacagttta
     1141 acagcgtcga tttcccgcaa ccgttaggac cgatcagggc ggtgatcttc cccgttggca
     1201 gtgagagtga aacgtcgtta agtaccttgt ctgttccgta attgaccgtc agattttcag
     1261 ttcgtaaagt catttatcgc attctcacaa gcaaccagac aaaccacggc gcaccgataa
     1321 tggcggtcag cacgccaacc gggagctcca gtgggggatg aataattctc gccagcagat
     1381 cggcaaccac caacagcaac gcacctgtca gggccgaaac aggcagcagt ctgcggtgac
     1441 gtccaccggt gatgctacgc atcatatgcg gcaccacgag accaataaag ctaatcgggc
     1501 cgcaggcggc cacgccggta gatgtcatgg cgacagctag taacaaagcc cagaatcggg
     1561 tatagggcac cgacacaccg agcgtggtgg cgcgcgcatc gccgagtgca aggaggtcga
     1621 gatcgcggca aaaactcagg ctcagcggca gaaataaaat catcagcggg atggcaatct
     1681 tcacaaagct ccagtcacgg ccccataagc tgccggtcag ccacagcagg gcgttgttca
     1741 catcctgcgg gcgcgagagc atcagataat ccgtcaggct ggcccagcat gcagaaagcg
     1801 ccacgccggt gagtgccagc ttcatcggct ggtgggtctt tgccagcatc ttcagcaata
     1861 tcaaccccgc catgccgccc gcaaaggcca gcagcggcag caccatcacg ggcagtgacg
     1921 gcataagaag tagagccccc acagaggcca ggctggcggc atggttaacg ccgagaatat
     1981 ccggtgatgc cagagggttg cgcacaatcc cctgtatcag cacgcccgcc acggcgaggg
     2041 ctgcaccgac aaacagtgcc agcagcaagc gcggcagtcg gtactccatc aatacataat
     2101 aatgctcgcg tccggcctgc cagtcggtca gcagcgcgcg ccacggcacg gggatcactc
     2161 ccatatggag tgataacagc gcacagcccg ccagggcaag ggtgatgaaa ataaccagcg
     2221 caattttcat cctcgcctcc tcacaagcca gacaaagcaa gggctgccaa tcagcgccag
     2281 cactgcgcct gcgggcagat ctccggggaa ggccagcgcg cgtgcgagta catctgccag
     2341 cagcatcagc gtggccccca gcagcatgct cactggcagt acgttgcgct gatcgaagcc
     2401 tgcccagaag cgcgccagat gtggcaccag cagaccgata aacgccaccg gacctgccac
     2461 gctgacgcac gcaccaacca gaagcagcac taacatattg atgaccaaac gtagcctcgt
     2521 caggttcact cccagcgtat gggcggtgct gtcgctgagg ttgagcaggt tcagttgatt
     2581 cgccagcagc aacacgacag ggactgcagt gaccaccacc ggcaagagct gccagacatc
     2641 ctgccagcgg gcgtgggaca ctccgccagc cagccagtaa aagatgccgt aagcatgatc
     2701 ttcggccagc agcagggtga tgcgggtaag gcccatacaa aaggccgaca gcgcgatacc
     2761 cgcgaggatc agtttgtttc tgtcatgggt atgacgaaat ccgcctcctg cggtcatgac
     2821 cagcagccag ctcacgccgc ccccgcatgc cgcgatgaac gacagagaat agcctgcaat
     2881 cggcgtcgga ctcagcgcgc tggtaagcgc catagccagc gccgcgccgc tgttaatgcc
     2941 gagcagtgaa ggagaggcca ttgggttgtg ggtcagggtt tgcagcagcg tgcccgcgag
     3001 cgccaggctt gcgccgatca gaacggcgac caggcttcgt ggcaaacgaa ggttttgcac
     3061 cagcgcttct ggtagcgttg gcgtgtgtcc aggcagcagg gcgcgggttg catctgctcc
     3121 ggaaacagga atggccgagt agcaaaacag actcagccag aaaataataa taagtgctgc
     3181 aacgggaagc ccccacagca gcaccgggtg ttttatcgcg gtcatttcac aacggtaagc
     3241 ggctgatggt ggaagatttt taccgtgtca gcggcaatac gctctgcagc aaaaataccg
     3301 cgcatccgcg cccaggtgtt actgtcgacc gaagcaacct gctgcttctg cgcggcggtt
     3361 aacatctgcc agagcggatc ttgttgccag cgtttaacaa tgctctcttc gcgatagtgg
     3421 gcaaccagca gccaggcagg attgaccgcc agcagttgct ccaggccgat ggacggcatg
     3481 gacgcacccg ccatcgcagc gggaacgttc agccccagag aggccagcac gctgccggtc
     3541 caggtctcct gagtatgcag gttgaattgc tgttcgcgtg atgtgccaaa ggccacgcgt
     3601 gtccctttgg gaagctggct ggcccactgc gccatcctct ctttatgttg ttccagacgt
     3661 gcctgcatct ctcgcttttt acccaccatt tcgccgatga tagccgcaga ttgcaaattt
     3721 tcagcgtagg tttcgttgcg ggacttaagc agcagtaccg acgcgatttg ctgcaaggcg
     3781 atgtaaaccc ccgcatggcg actgctgtcg gcaatgatca ggtctggttt cagagcggca
     3841 atggcttcca ggctcggctg cgcgcgcgtt ccgacggact gccacggttt caggtgcgca
     3901 cgcacttcgg gcaggatgcg ttttgcatcg ttatcgtcgg caataccgat cgggctgacg
     3961 tccacggcgg ccagcgcatc ggcgaacgag agttccagca ccacaatccg ttgtggcgtt
     4021 ttttcgagtg taaacgtgcc gtgttcgtcc tgaaccgtgg cggcaaaggc gtggctgatc
     4081 accagcagca ggcctgcaaa aagaaaacgg ataaatgcca acataatcac attccagcta
     4141 aaagcccggc aagccgggcg ttaacacatc agaacttcaa cgccccctgc atatacagcg
     4201 tgcgcggctg gcctgcatag atgcctttgt tgttgtcgtc ataagagcgg atgaagtagt
     4261 cctggtcgaa gatgtttttc acaccgaatg ccaggttcag atctgccatc tgcgggccaa
     4321 agtcatacac cacgcgtgcg ccccagagca tgaagccggg aatgcggccg gtactgccgt
     4381 cggcgctctc tttcaccgta ttggcgttat ccgcaaactg gctggactgg aaatcgctgt
     4441 tcagattgaa catccagttt cctggcttgt agtccacgcc cagcgtgcct ttatgtttcg
     4501 gggagaatgg taccagattg ccgtaggtgt cgcctttctc gcggatttcc gcgttcacat
     4561 acgcatagct ggcgtagatg gaaacgttat caagcgttgg cgttagcgta cccagatcgt
     4621 aacgtgcctg cgtttccagc ccggtatggc gcgttttgcc acgtgcagtg acggtgtcgt
     4681 tggtctggtt ggagtcgtac tgattgttaa agttaatcag gaacagcccc atttccgccg
     4741 tcagcgcgcc gtcgtcgtag cgggtaccga gttcccaggt tcgcgctttt tccggttcca
     4801 cattgccgct ttgcacagcc ttgccaatct ggctgtactg tacggtgccg aacgagcctt
     4861 cagtgtttgc ataaagattc cagctgtcag tcaggtgata gagcacgttc aacgccggaa
     4921 gcggtgcgtt atagctcact tcttcgtgcg tgcctgtgat ggcgttgttc tggtatgact
     4981 cgatatgttc gaaacgcata cccggcgtga tggtccagtt gccgatgtcg attttgtcat
     5041 ccagatacca ggcgtgcgcc tcggtgccgg aacgcgtatc gcggtcgtaa gggcttgagc
     5101 cggacggcaa ctgcccgctg ctggtggcgg tgtagtaacg catttcatgc gttgattcat
     5161 tcacatagcg atagcccacg cccacttcgt gcgcggaagg gccgatcata aagatctggc
     5221 tgtagcgtgg ctcaataccg cgcacccagt agttacgcgg cgagagggtg atgcgtttgc
     5281 cttgctccag gtagccgctg cgcagggttt gggtgtagaa cccctgaatg ttgaatttat
     5341 gctggctgtc tggctggaac tggtagccca ggctcgccag cttgcgacga ccccagaagc
     5401 ggtcatacgg gcgggtggat tgccagcgat cggcgtcgta atccgcgcga gacaggccac
     5461 cgggcatgtc ggcttcaccg tcgtaatatt gcagcaggct gttgaaggtg tgcacctcat
     5521 ccggcgcata tttgcttttc agcatcaggt cgtcgatgcg ggtggcgctg tgctcgcgcc
     5581 agtcactgcc gcgcgtgccg gagtagagca gcgcggtgcc aaaaccgttg tccgctgtgc
     5641 cgcccaccat caggttgtgc gtctctttcg ggttgttttg tgaagaggtt gggctgagct
     5701 gaccttccac gcccgcctcg ataccaaagt cctgcggaat ggcacgggta acaaagttca
     5761 ccacgccgcc cacgctctgc ggtccgtaac gcaccgcacc accaccgcgt accacgtcaa
     5821 tggcattcat gttgccgagc gaaacgggag ccagtgaaag ctgcggctga ccgtaagggg
     5881 cgaaggggac ggggatgccg tccatcagga cggtcgagcg gctggcgagg cgcgggttca
     5941 ggccccggat gccaaagttc atcgccaggt cgtggctgcc ggtgccgttg ttttccggcg
     6001 cgctgacgcc agggatgcgg ttaagtacct cacgcatggt ggttgcgccg gttttggcga
     6061 aatcctcacg gcggatcacg tcacgcgcgc cagcatgttc aaatacgtcg ttttcacgcg
     6121 catcacccag ccagtcgccg accacggtca gggcatcttc ttttggtgcg ggcgcgggct
     6181 ccagcgtcca gctgttattt cccagcggtt ttacctgcag tccgctgccg tccagcagtt
     6241 gttgcaggcc gctctcgacg tcgtaatcgc cgtgcaggcc gttgctctgc ttgccgcgcg
     6301 tcaggctggc gtcaaccgag agggtaaatc cgctgtgtgc ggcatactga ttgagcgctt
     6361 tatcgagcga tcccggtgcg atattaacct gtgcagcaaa agcggaaaac gagagaccgg
     6421 ccagcggcag caggctcagg cgaatggcgt taaccaaagg tgttgtttta cgaaaaacgc
     6481 gtaacggcgt cataccttcc ccatcatcat ttttgttgtg ttcagctatg agtcgaacga
     6541 gaagggtaaa aaggacaatc gaaataagaa ttattttcct tacagtggtg aaatgtttat
     6601 ccagtaccgc gtaatagact gaattttaac gggaagcgtt tgcgcgataa cgttcaggat
     6661 cgcatcggta tttttcagcg ggaacgtccc gctcaggcgc agcccggcaa cggcgggatc
     6721 gcagcgcagc acgccgttgc ggtaacgggt tagcgtggct atcacctcac ccagcggttt
     6781 atcgctgaag ctcaggatgt ccttcgtcca gcttgtactc tcgtcatcca gcggtttcac
     6841 tgcgccaaac tcagaggcgc tgaactgcag gctttcacca gcgttcacga tgcgtttttg
     6901 cgcgggggca ctggcgagaa gcacttccac ggcgtgctgc tgcacgtcaa gctgcgtgaa
     6961 attatcctgc tggcggacgg taaattctgt ccctaaagca gtgagctggc cctgacgggt
     7021 caggacacgg aaggggcgtt gcagggcatc tttcgcggta gtaatggcga tttcaccgta
     7081 ccagagccgg acggtgcgct gatgcgcatc aaaacgcata tccgccgcgc tttgggtatt
     7141 cagcgtgagc agggagccat cttccagttg ctgacggctg actgtgcctt tggcggtgcg
     7201 gtaatctgcc cgcagacctt cgccggtttc cgactgccag agctgccagc ctccgccagc
     7261 gccgagcaac agcagtaatc ctttcatcac gtgacggcgg gtgaggcggg tatcgtgcaa
     7321 cgcccggctg gcaacgtcgc caggcacacc gccaagctgg ttgcgcaggt tttcaacctg
     7381 ctgccaggcc cactggttat cctgatcctg ttcataccac tgttgccagc gcgcttcctg
     7441 ttgtgggctg acgcgctcgc cgcttagcac ggcataccag tgggaagctg aacgcagcgc
     7501 ctgacggcgg gaatcggtta acaaaggatt cataacccat actccagacg gaacagcagg
     7561 cagtgctcga cggctttcgc cacgtatttt ttcacggagc tgatggaaac accgagtttg
     7621 tgcgcaatct cgctgtatgt cagaccatcc agttgcgaaa gcagaaacgc ttcacgtgtt
     7681 ttgccgttta gcccgtccag catgctgtcg aggagttgta gggtctcgag ttggctttcg
     7741 cgttcctcag gtgaaggcgc tcccccctcc ggcataagcg ccagcatctc cagatacgct
     7801 ttttccagcg cgtttcggcg aaacaggtcc accatcacgc gtttggcgat agtgcagagg
     7861 aaggagcgag gatcgcggat cgtcgagagc gtttcgctga ccattacccg caaaaaagtg
     7921 tcctgggcaa tgtcatctgc atcaaaagca gactggagtt tgcgcgtcag ccagcttttc
     7981 aaccagccgt gatgtgtgcc ataaagcgac tcgaatgtta aggaagctgt ggtagtggcg
     8041 cggtcagaca tgcggagtgc atcaaaagtt aattatcacg tagccatatt aatatgagaa
     8101 tagttatcat tacaattgga aataaaattg tttccaatag acatttttaa catgttgttt
     8161 ttctaagtgt tataaggtag gtataaaatg ggatggagcc tctgcttctg gcatgtgtcg
     8221 gtcagaatga ctcatgatgt ggtctgctat tattgacatc ctcactgccc taaaggatag
     8281 ggatttcggt aatgctgtca acttactgat ttagtgtatg atggtgattt taaggtgctt
     8341 gcgtggcttc catttccatc agatgtcctt cctgctccgc tactgaaggc gtggtgcgta
     8401 acggcaaaag cactgccgga catcagcgct atctctgctc tcattgccgt aaaacatggc
     8461 aactacagtt cacttacacc gcctctcagc ccggtaagca ccagaaaatc attgatatgg
     8521 ccatgaatgg cgtcggatgt cgcgccagtg cacgcattat gggcgttggc ctcaacacgg
     8581 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac aaccgggcag
     8641 tgatgtgatt gtctgcgctg aaatggacga acagtggggc tacgtcggtg ctaaatcacg
     8701 tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg cgcacgtctt
     8761 cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg cctttgaggt
     8821 cgtggtatag atgacggatg gctggccgct gtatgaatca cgcctgaagg gaaagctgca
     8881 cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc tgagacaaca
     8941 tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc tgcatgacaa
     9001 ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca ttacctcctc
     9061 ccgaactcaa aacta
//
LOCUS       DAZPAC010000163         8986 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZPAC010000163 DAZPAC010000000
VERSION     DAZPAC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8986
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.163"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            124..1522
                     /gene="ulaA"
                     /locus_tag="ACHUPM_003219"
                     /pseudo
     CDS             124..1522
                     /gene="ulaA"
                     /locus_tag="ACHUPM_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            1538..1843
                     /gene="ulaB"
                     /locus_tag="ACHUPM_003220"
     CDS             1538..1843
                     /gene="ulaB"
                     /locus_tag="ACHUPM_003220"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR0870917.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1853..2317
                     /gene="ulaC"
                     /locus_tag="ACHUPM_003221"
     CDS             1853..2317
                     /gene="ulaC"
                     /locus_tag="ACHUPM_003221"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR0870918.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2331..2981
                     /gene="ulaD"
                     /locus_tag="ACHUPM_003222"
     CDS             2331..2981
                     /gene="ulaD"
                     /locus_tag="ACHUPM_003222"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR0870919.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2991..3845
                     /gene="ulaE"
                     /locus_tag="ACHUPM_003223"
     CDS             2991..3845
                     /gene="ulaE"
                     /locus_tag="ACHUPM_003223"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR0870920.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            3845..4531
                     /gene="ulaF"
                     /locus_tag="ACHUPM_003224"
     CDS             3845..4531
                     /gene="ulaF"
                     /locus_tag="ACHUPM_003224"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR0870921.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            4628..5179
                     /locus_tag="ACHUPM_003225"
     CDS             4628..5179
                     /locus_tag="ACHUPM_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFR0870922.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(5254..5569)
                     /locus_tag="ACHUPM_003226"
                     /pseudo
     CDS             complement(5254..5569)
                     /locus_tag="ACHUPM_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            5896..6291
                     /gene="rpsF"
                     /locus_tag="ACHUPM_003227"
     CDS             5896..6291
                     /gene="rpsF"
                     /locus_tag="ACHUPM_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR0870923.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            6298..6612
                     /gene="priB"
                     /locus_tag="ACHUPM_003228"
     CDS             6298..6612
                     /gene="priB"
                     /locus_tag="ACHUPM_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR0870924.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            6617..6844
                     /gene="rpsR"
                     /locus_tag="ACHUPM_003229"
     CDS             6617..6844
                     /gene="rpsR"
                     /locus_tag="ACHUPM_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR0870925.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6886..7335
                     /gene="rplI"
                     /locus_tag="ACHUPM_003230"
     CDS             6886..7335
                     /gene="rplI"
                     /locus_tag="ACHUPM_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR0870926.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(7447..8418)
                     /locus_tag="ACHUPM_003231"
     CDS             complement(7447..8418)
                     /locus_tag="ACHUPM_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR0870927.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(8471..8887)
                     /locus_tag="ACHUPM_003232"
                     /pseudo
     CDS             complement(8471..8887)
                     /locus_tag="ACHUPM_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            complement(8904..>8986)
                     /locus_tag="ACHUPM_003233"
     CDS             complement(8904..>8986)
                     /locus_tag="ACHUPM_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0870928.1"
                     /translation="KSLSFSKSVELHDKVIGYYLNIKHYQ"
BASE COUNT         2165 a         2197 c         2382 g         2242 t
ORIGIN      
        1 tttgcgggtc gcgtcacatt taatcataaa taatcttgtt gtgattactt ttgaaaatta
       61 gagtgagtgc acaacattcc gggtgtgcgg aatacccggt tacctcttct tcaggagatc
      121 gttatggaga tcctctacaa catctttacc gtgtttttta accaggtcat gaccaatgcc
      181 ccgttgttgc tgggtattgt gacctgtctg ggctacatcc tactgcgcaa aagtgtcagc
      241 gttattatta aaggcacgat taaaaccata attggtttca tgttgttgca ggcagggtcc
      301 ggcatcctca ccagcacctt caaaccggtg gtggcgaaaa tgtccgaagt ctacggcatt
      361 aaacggcgca atttccgata cttacgcgtc gatgatggca accatcgacc gcatgggcga
      421 tgcctatagc tgggtaggtt acgcggtgtt gttagcgctg gcgctgaaca tctgttacgt
      481 gctgctgcgt cgcattaccg gtattcgtac catcatgctg actggtcata tcatgttcca
      541 gcaggcgggg ctgattgccg ttacgttgtt tattttcggc tactccatgt ggacaaccat
      601 tatctgcacg gcgattctgg tttcgctcta ctggggcatt acctccaaca tgatgtacaa
      661 gccgactcag gaagtgacgg acggctgcgg tttctccatc ggtcaccagc aacagtttgc
      721 atcatggact gcctataaag ttgcgccgtt cctcggcaaa aaagaggaaa gcgttgaaga
      781 cctcaaactg ccaggctggc tgaatatttt ccacgacaac atcgtctcca cggcgattgt
      841 catgaccatc ttctttggtg ccattctgct ctccttcggt atcgacaccg tgcaggcgat
      901 ggcaggcaaa gtgcactgga cggtctatat cctgcaaacc ggtttttcct tcgcggtggc
      961 gatcttcatc atcactcagg gtgtgcgcat gtttgtggcg gaactctctg aagcgtttaa
     1021 cggtatctcc cagcgcctga tccctggtgc ggttctggcg attgactgtg cggctattta
     1081 tagcttcgcg ccgaacgccg tggtctgggg ctttatgtgg ggcaccatcg gtcagctgat
     1141 tgcggttggc atcctggtcg cctgcggttc ctcgagcctg attatcccag gctttatccc
     1201 gatgttcttc tccaacgcca ccatcggcgt gttcgctaac cacttcggcg gctggcgtgc
     1261 ggcgctgaag atttgtctgg tgatggggat gatcgaaatc tttggttgcg tctgggcggt
     1321 gaaacttacc ggtatgagtg cctggatggg gatggcggac tggtcgattc tggcaccgcc
     1381 gatgatgcag ggcttcttct ccatcggtat cgcctttatg gccgtcatca ttgtaattgc
     1441 actggcttat atgttcttcg ctggccgcgc actgcgcgca gaagaagatg cagaaaaaca
     1501 actggcagaa cagtctgctt aataaggagt tttgattatg accgtacgta ttctggctgt
     1561 gtgtggcaac ggacaaggca gttccatgat catgaggatg aaagtggacc agtttttaac
     1621 ccaatcaaac attgaccaca cggtaaatag ctgcgcggtt ggcgaataca aaagcgagtt
     1681 gagcggcgcg gatatcatca tcgcttctac gcacattgcg ggcgaaatca ccgtgaccgg
     1741 caacaaatac gtggttggcg tgcgcaacat gctctctcct gccgactttg gcccgaaact
     1801 gctggaagtg atcaaagcgc atttcccgca ggatgtgaag taaggacgcg ccatgaaatt
     1861 acgtgattcg ctggcggaga ataaatccat ccgcctgcag gctgaagctg agacatggca
     1921 ggacgcagta aaaatcggcg ttgacctgct ggtggcggcg gatgttgtcg agccgcgtta
     1981 ctaccaggcg attctggatg cggttgaaca gcacggtccg tactttgtgc tcgcaccagg
     2041 cctggcaatg ccgcacggtc gcccggaaga gggcgttaag aaaaccggtt ttgcgctggt
     2101 gacgctgaaa aagccgctgg agttcaacca cgaagataac gacccggtgg atatcctcat
     2161 caccatggcg gcggtcgatg ccaacacgca ccaggaagtg ggcatcatgc agatcgtcaa
     2221 cctgtttgaa gatgaagaaa atttcgaccg tttacgtgcc tgccgtaccg agcaggaagt
     2281 actggatctc attgaccgca ccaacgcggc agcttaagaa ggaattgaag atgtcattac
     2341 cgatgttgca agtcgcgctg gacaaccaga ctatggatag cgcctacgaa accactcgcc
     2401 tgattgccga agaagtcgac attatcgaag tgggtaccat tctgtgcgtg ggcgaaggcg
     2461 tgcgtgcggt tcgcgatctg aaagcgctct acccgcacaa aatcgtgctg gcagacgcca
     2521 aaattgccga tgcgggcaaa attctttctc gtatgtgctt cgaagccaac gctgactggg
     2581 tgaccgtgat ttgttgtgcg gatatcaaca ccgccaaagg tgcgctggac gtagcaaaag
     2641 agtttaacgg cgacgtgcag atcgaactga ccggttactg gacctgggaa caggcgcaac
     2701 agtggcgcga cgcaggtatt cagcaggtgg tttatcaccg cagccgtgac gcgcaggccg
     2761 ctggcgtggc gtggggtgaa gccgatatca ctgcgatcaa acgtctttcc gatatgggct
     2821 tcaaagtcac cgtcaccggt ggcctggcgc tggaagatct gccgctgttc aaaggcattc
     2881 caattcatgt gtttatcgcc ggtcgcagca tccgtgatgc cgcttctccg gttgaagccg
     2941 cgcgtcagtt caaacgttcc atcgccgaac tgtggggcta aggagcggat atgttgtcca
     3001 aacaaatccc gcttggcatc tatgaaaaag cgctccccgc cggggagtgc tggctggaac
     3061 gcctgcgact ggcaaaaacg ttgggcttcg attttgtcga gatgtcggta gatgaaactg
     3121 acgagcgcct gtcgcgcctc gactggagcc gcgagcagcg tctggcgctg gtgaatgcga
     3181 ttgttgaaac cggcgtgcgt gtgccgtcca tgtgcctttc tgcccatcgt cgtttcccgt
     3241 tgggcagcga agatgacgcg gtgcgggcgc aggggctgga gattatgcgt aaagccatcc
     3301 agttcgccca ggacgttggc attcgcgtga tccagctggc gggctatgac gtttactatc
     3361 aggaagccaa taacgaaacg cgtcgtcgtt tccgtgacgg cctgaaagag agcgttgaga
     3421 tggcgagccg cgcgcaggtg acgctggcga tggagatcat ggattatccg ttgatgaact
     3481 ccatcagcaa ggcgctgggt tacgcgcact atctcaacaa cccgtggttc cagctctacc
     3541 cggatatcgg caacctgtcg gcgtgggaca acgatgtgca gatggagttg caggccggaa
     3601 tcgggcatat cgtcgcggtg catgtgaaag acaccaaacc gggcgtcttc aaaaacgtgc
     3661 catttggcga aggtgtggtg gatttcgaac gttgcttcga aacgctcaaa cagagtggct
     3721 attgcgggcc gtacctgatt gagatgtgga gcgaaacggc ggaagacccg gcggcagaag
     3781 tggtgaaagc gcgtgattgg gtgaaagcgc gcatggccaa agcaggcacg gtggaggcgg
     3841 cataatgcaa aagctaaaac agcaggtgtt tgaagccaac atggatctgc cgcgctatgg
     3901 gctggtgacc tttacctggg gcaacgtcag cgctatcgac cgcgaacgcg ggctggtggt
     3961 gatcaagccc agcggcgttg cctacgaaac catgaaagca gacgatatgg tggtggtcga
     4021 tatgagcggc aaggtggtgg aaggggagta tcgcccgtct tccgacaccg cgacgtatct
     4081 cgaactctac cgtcgttacc cgtcgcttgg cggcattgtc catacccact ccactcatgc
     4141 tactgcatgg gcgcaggcgg ggctggcgat cccggcgtta ggcactacgc acgccgacta
     4201 cttctttggc gacatcccgt gtacgcgcgg attaagcgaa gaagaggtgc agggcgagta
     4261 tgaactgaac accggcaaag tgattatcga aacgctgggt gacgccgagc cgctgcatac
     4321 gccggggatt gtggtgtatc agcacggtcc gttcgcctgg gggaaagatg cccacgacgc
     4381 ggtgcataac gcggtggtga tggaagaagt ggcgaaaatg gcgtggattg cgcgcagtat
     4441 taacccacaa ctgaatcaca tcgacagctt cctcatgaat aaacacttca tgcgtaagca
     4501 cggtcctaac gcctattacg ggcagaagta agcagtgcgc cccggaatta ttacctcctg
     4561 cgggatgtaa ttggtctgat tccggggccg cttcagttaa aaaatcgtat aaacaacagg
     4621 tgtttttatg gctatcaatg gattgtttgt cagaacggat ccgcaacgtc gccgctatgg
     4681 cgttgcgtta cttgtgggtg tactttcagg aattatttca gcatttgtta aatggggagc
     4741 ggaagtcccc ttgccaccgc gcacattttc cggtggtcgc gatgaattta acccgccgta
     4801 tatctttcta cgggattatt taggtatcga cccaacccaa actgtgtata cattctcgga
     4861 acatattatc aacccggtga tggtgacgca tatcatcttc tcactggttt ttgcgatagg
     4921 gtattgtatt gtcgcggaaa ttttcccgaa agtgaaatta tggcagggga tattagcagg
     4981 gcttattgtg acagtggcag tccacggtat attttgcccg gcgctgaatt taacgccgcc
     5041 gctcactcaa ttgccttttg acgaatatgc ctctgaaatc ctggggcata ttttctggtt
     5101 ctggataata gaaattatgc gccgcgactt acgtaatcgc attactcatg agccagatcc
     5161 ggaagtcgca atccggtgaa taaccgcata aaaaagcccc ctgcgaaggg ggcaaagcaa
     5221 actatggcaa tgtttcgttg gttatacctg gtgctagcga taaatatccg cgctggcgtg
     5281 catattgccg ttactccccg gttcccgcat cagaattacg tggtagtacg ttactccccg
     5341 gttcccgcat cagaattacg tggtagtacg ttgcgccctg cgcatctgtc tcttcattta
     5401 atgcctgacg cgcttcgctt tcagtggcga aattatgatt gatataaatc acgcctaagc
     5461 tttgcacatc atccatgttt ctggcctggt ggttattaat ttcaatggct gcccatgtat
     5521 ttgcacttag caaaagcaca gccagaaggg ctaaaactcg actgaacata gatacctcct
     5581 cgacggctga ctttgtgtgc tctcctctgt gatgatcttc tgatttaatt ttaatcaatg
     5641 ataaagaagt tgatggtgac catttctgat gcagttgttc aaaaaaacac catgatgaag
     5701 tgtgatgaac tccaaatcag cggtttagag gttaattgcg aaaggggaga tttatttcgg
     5761 ctctgccctt gagtttagcg gggcatacaa gtactataac gcgtcatttt tcagccgacc
     5821 tttaacacgt tccttgcctc cccgggattc ggctgaccca gacaggaggc tgaataatcc
     5881 gtaaggagca attcgatgcg tcattacgaa atcgttttta tggtccatcc tgaccagagc
     5941 gaacaggttc cgggcatgat cgagcgctac actgctgcca tcactggtgc agaaggcaag
     6001 atccaccgtc tggaagactg gggccgccgt cagctggctt acccgatcaa caaactgcac
     6061 aaagcacact acgttctgat gaacgttgaa gctccgcagg aagtgatcga tgagctggaa
     6121 actaccttcc gcttcaacga tgccgttatc cgcagcatgg ttatgcgtac caagcacgct
     6181 gttaccgaag catctccgat ggttaaagcg aaagacgagc gccgtgagcg tcgcgatgat
     6241 ttcgcaaacg aaaccgctga tgatgctgaa gctggggatt ctgaagagta atttctgatg
     6301 accaaccgtc tggtgttgtc cggcaccgtg tgcaggactc cccttcgaaa ggtcagtcca
     6361 tcaggaattc ctcactgcca gttcgtgctt gagcatcgtt ctgtgcagga ggaagccggt
     6421 tttcaccggc aggcgtggtg ccaaatgccc gttattgtta gcggacacga aaaccaggcc
     6481 attactcaca gtataacggt cggcagtcgc ataaccgttc aggggttcat ttcatgccac
     6541 aaggcaaaga acggactgag caaaatggtt ttgcatgccg agcagattga attgatagat
     6601 tctggagact agccatatgg cacgttattt ccgtcgtcgc aagttctgcc gtttcaccgc
     6661 ggaaggcgtt caagagatcg actataaaga tatcgctacg ctgaaaaact acatcaccga
     6721 aagcggtaag attgtcccaa gccgtatcac cggtacccgt gcaaaatacc agcgtcagct
     6781 ggctcgcgct atcaaacgcg ctcgctacct gtccctgctg ccgtacactg atcgccatca
     6841 gtaatcggtc acggtccatt aatacgactt tgagaggata aggtaatgca agttattctg
     6901 cttgataaag tagcaaacct gggtagcctg ggtgatcagg taaacgttaa agcgggctat
     6961 gctcgtaact tcctggtacc gcagggtaaa gctgttccag ctaccaagaa aaacattgaa
     7021 ttcttcgaag cacgtcgcgc tgaactggaa gctaaactgg ctgaagttct ggcagctgct
     7081 aatgctcgcg ctgagaaaat caatgcactg gaaactgtta ccatcgcgtc taaagctggc
     7141 gacgaaggta aactgttcgg ttccatcggt actcgcgaca tcgctgacgc tgtaactgca
     7201 gctggcgttg aagtggctaa gagcgaagtt cgtctgccga acggcgttct gcgtaccact
     7261 ggcgagcacg aagtgagctt ccaggttcac agcgaagtat tcgcgaaagt gatcgtaaac
     7321 gtagtagctg aataattcgt tattcaacga gacgtaaaaa cgccgaccag tggtcggcgt
     7381 tttgcttttg tagcgagcac ttgcagatat cgataagggc cacaataagc agccctacga
     7441 agataattac ttagctgcct gaatatcagc cagcattggg gcaagggctg ggttgtcctt
     7501 agcgaagttt gcgtacattg gctgtacttt ctcgataaac ggagctttat ctacctggta
     7561 gactttcacg cccattttat ccagttttgc aaattcttgt ttctcttcct cgctccacaa
     7621 gtctttcatg tagctcattg attctttagc tgcttttaat ataactgcct gattttccgg
     7681 gctaagttta tcaaacgcag cattgctcat cagaaccaca tcgggaacca tagtgtgttc
     7741 gtcaaggctg aaggattttg ccacttcacc gtgacgcatg gtggtcagcg ccatcacgct
     7801 gttttctgcc atatcgacca caccctgctg taaggctgta tacaactcgc cgtaatccag
     7861 tggcgtcggg ttaccgccca tgactttgat catttcaact gcgccagggc ttggctggac
     7921 acggactttc atgcctttca gatcgtctgg tttcagtact ggtttattgc catagaaact
     7981 gcgggcgcct ccatcataaa aagtcagccc gaaataacct ttgcttttcg ttgagtcgag
     8041 gatttttctc ccgatatcgc cctgcatgac ctgataataa tgatcacgat cgcggaacaa
     8101 ataaggcagg ctaaataatt tataggaatt ttcaaatgct tccatttctg ccgcattggt
     8161 ttttaccagt ggaatagcgc cagaacgaat cagctccatt gtttctcgtt gagtacccaa
     8221 tgttccattt ggataaatac gaatagtaat atcaccgtta gagtactctt tgttcttttt
     8281 cgcaaagaac tccatcgctt tatgaacagg atgagactta tcctgattat gactcagttt
     8341 taatgtcact gcccaggact ggcttgcgga taacataaga acagcaccga taatgccagc
     8401 ggatactttt attttcatag atgttccttt tctaataaat atggcggcag gtgccgccag
     8461 tgaatgcgca tcagtagccc agtgcttttg gcaatgccat actgatttgc ggaatatagg
     8521 tcaccattgc cagcaccaat accatcacgg catagaatgg cagcataggt ttgataattt
     8581 tattaatcgg taatttagat acactacaac cgacaaataa cacactgcct acaggcggag
     8641 tacaaatacc gatacacatg ttcagtacca tgacaatacc aaagtgaatc gggtccatgc
     8701 ccagttcggt gacaacaggt aaaaatatcg gcgtaaaaat cagtaccgcc ggtgtcatat
     8761 ccataaaagt gccgataatc aacaggataa tattgataat aagcaggatt attattgggt
     8821 tgtcagaaat agtgttaagc aaatcgagaa tcaggaaggg aacgtcagcg tttgacattg
     8881 cccatgacgg taatgactcc aacttattga tagtgtttta tgttcagata atacccgatg
     8941 actttgtcat gcagctccac cgattttgag aacgacagcg acttcc
//
LOCUS       DAZPAC010000164         8974 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZPAC010000164 DAZPAC010000000
VERSION     DAZPAC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.164"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(180..938)
                     /gene="chbG"
                     /locus_tag="ACHUPM_003234"
     CDS             complement(180..938)
                     /gene="chbG"
                     /locus_tag="ACHUPM_003234"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR0870929.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(951..2303)
                     /gene="celF"
                     /locus_tag="ACHUPM_003235"
                     /gene_synonym="chbF"
     CDS             complement(951..2303)
                     /gene="celF"
                     /locus_tag="ACHUPM_003235"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR0870930.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(2408..3250)
                     /gene="chbR"
                     /locus_tag="ACHUPM_003236"
     CDS             complement(2408..3250)
                     /gene="chbR"
                     /locus_tag="ACHUPM_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFR0870931.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(3258..3608)
                     /gene="chbA"
                     /locus_tag="ACHUPM_003237"
     CDS             complement(3258..3608)
                     /gene="chbA"
                     /locus_tag="ACHUPM_003237"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR0870932.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(3659..5017)
                     /gene="chbC"
                     /locus_tag="ACHUPM_003238"
     CDS             complement(3659..5017)
                     /gene="chbC"
                     /locus_tag="ACHUPM_003238"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFR0870933.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(5102..5422)
                     /gene="chbB"
                     /locus_tag="ACHUPM_003239"
     CDS             complement(5102..5422)
                     /gene="chbB"
                     /locus_tag="ACHUPM_003239"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR0870934.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(5721..6059)
                     /gene="osmE"
                     /locus_tag="ACHUPM_003240"
     CDS             complement(5721..6059)
                     /gene="osmE"
                     /locus_tag="ACHUPM_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR0870935.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            6261..7088
                     /gene="nadE"
                     /locus_tag="ACHUPM_003241"
     CDS             6261..7088
                     /gene="nadE"
                     /locus_tag="ACHUPM_003241"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR0870936.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            7318..8205
                     /gene="cho"
                     /locus_tag="ACHUPM_003242"
     CDS             7318..8205
                     /gene="cho"
                     /locus_tag="ACHUPM_003242"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR0870937.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(8165..8740)
                     /gene="ves"
                     /locus_tag="ACHUPM_003243"
     CDS             complement(8165..8740)
                     /gene="ves"
                     /locus_tag="ACHUPM_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR0870938.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         2402 a         2292 c         1997 g         2283 t
ORIGIN      
        1 tatccggcct acaaaactgt gcaaattcaa tatattgcag gaaacacgta ggcctgataa
       61 gcgaagcgca tcaggcagtt ttgcgtttgt cagcagtctc aagcggcgca gttacgccgc
      121 ctttgtagga attaatcgcc ggatgcaagg ttcacgccgc atctggcaaa catcctcact
      181 tacacatccc gataactccc caaccgataa ccacgctgag cgatagcacc tttcaacgac
      241 gctgatgtca acacatccag ctccgttaag cgtgggaaac agtaagcact ctgacggata
      301 gtattatcga taaacgccgg atgacacatc acctccagcg aacgatcacc ccgatggctt
      361 gcgtcatcca gcacttgcag gaacagtgcc tcgctaatct cttcaccata aaacgcgcta
      421 ctgaatccct gagaactgcg cagattagcc ggtaaatcac cagagttaga tagcggctga
      481 cggtcgatgc gcaacgcaat cccctcttca gccgcaaacc tggcaacaat cgggaaaatc
      541 tgcgggaaca tatgcacatg atgatggcta tcaagatgcg taggtttgcg tccaaatagc
      601 tcaatgaaac gcaaatactg actggcaagc tcctgagtaa tttcttccag cggtaaagca
      661 tcttcttctg ccaactgcca gatccatttt cccagcacac catcgcgggt taaccccggc
      721 atagctgtca gtggcttgcc catagtaagg acaaagtaca tccctatggc cagactcggt
      781 tcatcacgac tcaactgcac cgcatggtca atagcctgcc cattcaccag cgccgtcgtc
      841 gatgtgacaa tcccattgcg acaggcctcg ataatgccgt agttctgacc tttgcttaag
      901 ccaaaatcat cggcattaac aatcagtaag cgttccataa tcagcctcgg ttaatgtgct
      961 tttttaagct ctgcgatgca gtcggcaaaa tttggcagcc atttctcgtg cgccagaatc
     1021 atctcgcgtg ccagcagctc agcatcgcga tcggaatgca ccaacggact aaggtttagc
     1081 gccagtaaca catcgttaaa ttctccgcta agtgcggcgt tgctggcagc aatctcgaag
     1141 cctttaatgg tgtgaatcag ccccatcact ttatcatcga aatgcgtaat gcgcggatgt
     1201 ggcgtcgcgc catcgcgccc cagcttacag gtcatttcta ccgcccagtc tgccggaata
     1261 ttatcaatat gcccatgatg cgggatatta acgtaatgtt cagcttgctt gtcgttgtag
     1321 atagcgttga tcacttcgca cgctgcatca gagtaataag ccccaccgcg ctgttccagt
     1381 tctttcggct taactttcaa ctccgggttt ttatacagct caaaaagttg tttctcgact
     1441 ttctgtacta cctgcgctcg tgcgccgcct ttgtagtatt cgcccatttc aatagccagc
     1501 atctcttttt gcttgaagta ataaagcaaa taagaacacg gcaacagatt cagagaacga
     1561 attaagcctt cgctaaatgg cagatcgaaa atatttttaa cgccagatgc ttttaactgc
     1621 ccggatgcca caccatcaag caattcggca aagcgcgact tgccatttac cagcacatcc
     1681 ttaatgaaca ccatatggtt gaggccgaac aaatcgatag ataaatcatc gctgtctttc
     1741 agcatcagaa catcgtgaat aaacatcttc atgccgatcg gaatattaca cacgccgata
     1801 aagcgtttaa atccggtatg acgataaacg gcttcagtaa ccattcccgc cgggttagtg
     1861 aagttaatca cccatgcatt cggacaaagt tcttcgacat cttttacgat gtcaaaaatc
     1921 accggaatgg tacgcagacc tttaaacaga ccgcccgcgc cgttggtttc ctggccaaga
     1981 taaccatgac ttaatggaat acgttcatcc agttcgcgcg ccggtaattg gcctacgcgc
     2041 agttgggtag taacgaaatc agcatctttc aatgcttcgc ggcgatccag cgttttataa
     2101 agcttcatcg ggacgccagc gttatcaatc atccgttggc agagttcaaa aatgatatcc
     2161 agtttctctt taccatcttc gacatccacc agccataatt cgctgaccgg caattcgtga
     2221 taacgcttaa taaatccttc cagtaactcc ggggtatagc tgctcccgcc accaatagtg
     2281 acgactttta atttctggct cataatttct cccttcagta cagaatactg atatctggca
     2341 tatctgcccc cccggacata aataatccag caacaggaca gatatgtgaa ttgtcaggta
     2401 taacgactta ctgattaaat tcagtcaatt tcttacgata gctcttaggc gtaaaggacg
     2461 ttaatttctt aaacgtttta ataaacaaac tcgggctact ataaccggcc tcaaacgcaa
     2521 tatccgtcac ggaatagttg gtcatttcca gttgtttttt ggcaaaatta atacggatct
     2581 cattaataat ctgcattggt gttttgccat aatatcgttg agtcgctcgc gtcaaatatt
     2641 cctgtgattt ggctgacaac gtcaccatat tctccagcgc cgattcacta aactgctctt
     2701 tatcatgcat cttttctacc gtacttttca gccactgcgg tacatcatca atcacctgtt
     2761 cttcgcggta atgacgtaaa cggttaatga cataaaaagt aaccatctca acaaactctt
     2821 ccagccctgt ttcgcggaaa ttcaatgaag aaatcactgt ttccacatag gtgagaaaag
     2881 cgttattggt ccggtatacc tgcgaagcga caaagcaata aggcaacaac ggcaggtaat
     2941 gctgctcaaa aaagcgttta ctgatcccaa cgttcaatat acgcgtggca ccaaactcat
     3001 aaaaactttg atggtgcgaa cctaacggaa taaaaacaaa atcgccccgt tccagtaaca
     3061 cgcgcttacc gttaatctcc tggaaataac gcccggttaa taccagagta aattcataat
     3121 agtcgtgctg atgcagcccg ctgatactct cagttttgtt atagataaac acatggaagt
     3181 ttttgccatt aaacaactgc tgttctcggg cagtggcaat ttccggcgcg ttaatcactg
     3241 gctgcatcat cgactcctta tgccttcagt ttttcatgaa gctcaattaa ttcagtaatc
     3301 agttcacgcg caagcatgga cgtcattaaa tgatcctgag cgtggaccag caccagactc
     3361 actttcatct taccttcgcc cgcatcgcct tcaatcagtt tcgtctgtac cagatgcgct
     3421 tcattcaatg ccatccgtga ctgatccatc atggcttttg ctgcagcaaa atcgccctgc
     3481 ttcgcctgtt tcagtgccgc atacgccagg ctgcgcgctt gtccggagtt gatgataagc
     3541 cccatcacca cttcttccag ctcttcagct tccgtttgcg tatcgggaat gttatcgaga
     3601 tccatcatac atcgttcctc tctattctta ccggcacgat tacccgtacc ggcatcgatt
     3661 aaaatttcag ggcgttagcg atatcttctt cgcgctcttc tttatcaatc gcgttttgtg
     3721 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatacca aggttgaaga
     3781 gtgcaaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     3841 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     3901 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     3961 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     4021 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     4081 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     4141 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     4201 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     4261 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     4321 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     4381 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     4441 cgggaattaa tgccgagaag gaacgcgata ctgaagcagg tacgctatcg ggcaatttaa
     4501 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     4561 tcccggagat gatattcgct ccacctaacc agtttgcgcc aaccacatag gcctcaccga
     4621 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     4681 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     4741 tccctaatgt tccgttatat acgttgccgc caataccttt caggccatta agtgtttcaa
     4801 tggttgaggc gtcgaggcga atacctaagg aataaaaaaa cgccccctcc ccaaagctta
     4861 gaaaaacgtt gttaattaat acaaacatgg cccccgcaag ggttaacggc attaagcgaa
     4921 taaagccatt tttgattgca ttaacgtgtg gctgctttcc tattttaact gcaaaaggga
     4981 ggagtacctt ttcaagcgat gcaataacat tactcataga aaaataccct taaaaaccgc
     5041 aatttaaata ttgcggtatt gatttatgaa ataactcttt gacgggaaaa tttaaaataa
     5101 attaatttgc tgcggctttt ttaatcgctg caaccgcagc cttaagcacg cctaaaccat
     5161 cgactttgcc ataaagcagc gagtcaatta cttcaaccgg tttgttgggt aacaaacgct
     5221 ggatttcggg caacatataa gcaatctgcg gccctaataa cacgacatcg gcattctgac
     5281 ctttttcacc agccagtgtt tccggaaatg cttcaataat gaccggaact tcatattttt
     5341 ctgcctgtgc gcgcattttt gataccagta aagaggtaga catgcccgca gaacaaaaca
     5401 gataaatgtg tttcttttcc ataaaactgc cctcatcgac gattatctgt cagccagaca
     5461 ctccgcaagc cttaacctgc ttccatgctc tgggtaactt gcgaaaccaa catgactttt
     5521 tttgtggctg aaaggagtat acggtataga cctgagcaat agtatttctt tgaccttata
     5581 aattgtttct ccttgacctg tggttatgac cctcttcaca tttcgggcaa ataattcgcg
     5641 aagataatta agaactcagg ccaaaaaaaa ccggcgcaat ggccggtttc cgttgttact
     5701 caagctttca gacgaattga ttacttcgca gcctgtggat cagtgtcgta ttcagcacag
     5761 gtctgataac cggagttgat gacatgtccg gtatcatcta acgcgacaaa gtaggtttct
     5821 gctttaccat cacgttgacc caggatgtag gtctggcaag taccacgagc atggatcatg
     5881 ctcacttcag acgaaggttt acccgcaatt tgtgcaacct gcgcccggct catgcctttt
     5941 ttgacgtctt tcaccacagg ctgtacaaac tggtctttag tacgatcata agccgtacaa
     6001 cccgccagca tggttaatac cgccgctgca ctcagaattc ctgccatatt cttgttcata
     6061 ttccgtcctc ttgtttatca gcgtgttaga taagcctgga atacattggg cgctttttca
     6121 agcccgcgaa cgaaacggct ccgctttcag aggattcctg tatgacgttt taaccaccat
     6181 tcagcccgct gtcgcttgtc gtttcagtgg caacgggtta gctttaagga agttttgtct
     6241 tttctgtctg gaggggttca atgacattgc aacaacaaat aataaaggcg ctgggcgcaa
     6301 aaccgcagat taatgctgaa gaggaaattc gtcgtagtat cgattttctg aaaagctacc
     6361 tgcaaactta tccgttcatt aaatcactgg tgctcgggat cagcggcggt caggactcca
     6421 cgcttgccgg aaagctgtgc cagatggcga ttaatgagct gcgcctggaa accggcaacg
     6481 aatcactgca atttattgcc gtacgcctgc cctatggtgt tcaggccgac gaacaagatt
     6541 gccaggatgc cattgccttt attcaaccgg atcgcgtatt aaccgttaat atcaagggcg
     6601 cggtattggc cagcgaacag gcattgcggg aagcaggcat tgaactgagc gattttgtcc
     6661 gtggcaatga aaaagcgcgt gagcggatga aagcacaata tagcattgcg ggtatgacca
     6721 gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat taccggattc ttcactaaat
     6781 atggtgacgg cggtacagac attaatccgc tgtatcgtct caacaaacgt cagggtaaac
     6841 agttactggc ggcattaggt tgcccggaac acctttataa gaaagcgcca acggccgatc
     6901 tggaagatga tcgcccttct ctgccagatg aagtggcact cggcgtgacc tatgacaata
     6961 tcgacgacta tctggaaggg aaaaacgtac ctgaacaggt cgccagaaca atagagaact
     7021 ggtatctgaa aaccgaacat aaacgccgtc cgccaattac cgttttcgat gatttctgga
     7081 aaaagtaata atttgcacat aatggtttgt gcgaaaaaga gtaatttgtt cacgccggat
     7141 gcggcgtgaa cgccttattc gacctacaaa actatgcaaa ttcaatatat tgcaggagtc
     7201 gaataggcct gacaggcgta gcacgtcaga cggtgtaacc tttgtcatcg acccgcctct
     7261 tttttaatcg ctccccgcct gttacactgg atagataacc agcattcgga gtcaacagtg
     7321 gtacggcgtt taacttctcc gcggctcgaa tttgaagctg cggcaattta tgaatatccc
     7381 gaacatttac gttcattcct taatgactta cccacccgac caggggtgta tctgtttcat
     7441 ggcgaaagtg acaccatgcc gctctatatc ggcaaaagcg ttaatatccg cagtcgggtg
     7501 ctttctcatt tacgcacccc ggatgaagcc gccatgctac ggcaatcccg gcgaattagc
     7561 tggatctgta ccgcaggcga aatcggcgct ctgctccttg aagcgcgatt aatcaaagaa
     7621 caacagccgc tgtttaataa acggttgcgc cgcaatcgcc agctctgtgc cctgcaatta
     7681 aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg atttttcacg agcccccaac
     7741 ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag cattgcagac catcgctgat
     7801 gaacaaaaac tttgttatgg cctgctggga ctggaaccgt taagtcgcgg tcgtgcatgt
     7861 tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg gtaaagagag ccatgaggaa
     7921 catgcgctac gcttgcgcca gtctctggag cgtttgcggg tggtgtgttg gccttggcaa
     7981 ggtgcggtgg cactgaaaga acagcacccg gaaatgactc aatatcatat tattcaaaac
     8041 tggctgtggc tgggggcggt taattcgctg gaagaagcga caacgttaat tcggacaccc
     8101 gccgggtttg atcacgacgg ttataaaatt ctttgtaagc cgctgctttc cggtaactat
     8161 gaaattactg aacttgatcc ggcgaatgac cagcgagcca gttgatttca ctgaacaaca
     8221 gtttaccttg cggttgtaaa agccgcaggg tgtgcctgcc atcgaaccag cacgcgccct
     8281 ggtcggtagt gagcaattta tcgcccagtt gccacgcccc gttaatcaca aacaccaccc
     8341 cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat tctgactttc gccttacaga
     8401 catccaggcg tgtcatgatg ttgaaatcca tcgacatctg cccttccgtc agtttcgctt
     8461 ttaccacctg gtccgctgca aaggcaaaag gctgtaacgg ttttaaggta tggttaaagc
     8521 ggtctgcgct ttcaaggagc atctcgccgc cttccagcaa cgtcactatc ctttccatgc
     8581 cgggaaacaa agaaaactcc ccattcgccg cgatggatgc aatgctggca cgccagtaaa
     8641 aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt ttcaccggca gcatttcgcc
     8701 acagattcac cgacatttta cgcatatcaa agtattccat actcgctccc ggcccgttct
     8761 tttcattgtt atcgttcagg taaccgtgga tggttatcgg caactcgggt gatttacgtt
     8821 agtggtgatc aggctttttt tacaactgtt tgagaagaga aaagaaaacc gccgatcctg
     8881 tccaccgcat tactgcaagg tagtggacaa gaccggcggt cttaagtttt ttggctgaaa
     8941 gattattcag cagttgcagg cattttacct tttg
//
LOCUS       DAZPAC010000165         8935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZPAC010000165 DAZPAC010000000
VERSION     DAZPAC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.165"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            8..832
                     /gene="gadX"
                     /locus_tag="ACHUPM_003244"
     CDS             8..832
                     /gene="gadX"
                     /locus_tag="ACHUPM_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR0870939.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1200..1928
                     /gene="gadW"
                     /locus_tag="ACHUPM_003245"
     CDS             1200..1928
                     /gene="gadW"
                     /locus_tag="ACHUPM_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR0870940.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2291..5404)
                     /gene="mdtF"
                     /locus_tag="ACHUPM_003246"
     CDS             complement(2291..5404)
                     /gene="mdtF"
                     /locus_tag="ACHUPM_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFR0870941.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5429..6538)
                     /gene="mdtE"
                     /locus_tag="ACHUPM_003247"
     CDS             complement(5429..6538)
                     /gene="mdtE"
                     /locus_tag="ACHUPM_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFR0870942.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6646..6924
                     /locus_tag="ACHUPM_003248"
     CDS             6646..6924
                     /locus_tag="ACHUPM_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870943.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6925..7452)
                     /gene="gadE"
                     /locus_tag="ACHUPM_003249"
     CDS             complement(6925..7452)
                     /gene="gadE"
                     /locus_tag="ACHUPM_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR0870944.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8251..8823)
                     /gene="hdeD"
                     /locus_tag="ACHUPM_003250"
     CDS             complement(8251..8823)
                     /gene="hdeD"
                     /locus_tag="ACHUPM_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR0870945.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2407 a         2078 c         2076 g         2374 t
ORIGIN      
        1 gtcaactatg caatcattac atgggaattg tctaattgcg tacgcaagac ataaatatat
       61 tctcaccatg gttaatggtg aatatcgcta ttttaatggc ggtgacctgg tttttgcgga
      121 tgcaagccaa attcgagtag ataagtgtgt tgaaaatttt gtattagtat caagggatac
      181 gctttcatta tttctgccga tgctcaagga ggaggcatta aatcttcatg cacataaaaa
      241 aatttcttca ttactcgttc atcactgtag cagagatatt cctgtttttc aggaagttgc
      301 gcaactatcg cagaataaga atcttcgcta tgcagaaatg ctacgtaaaa gagcattaat
      361 ctttgcgttg ttatctgttt ttcttgagga tgagcacttt ataccgctgc ttttgaacgt
      421 tttgcaaccg aacatgcgaa cacgagtttg tacggttatc aataataata tcgcccatga
      481 gtggacacta gcccgaatcg ccagcgagct gttgatgagt ccaagcctgt taaagaaaaa
      541 attgcgcgaa gaagagacat catattcaca gttgcttact gagtgtagaa tgcaacgtgc
      601 tttgcaactt attgttatac atggcttttc aattaagcga gtcgcagtgt cctgtggata
      661 tcacagcgtg tcgtatttca tttacgtctt tcgaaattat tatgggatga cgcccacaga
      721 gtatcaggag cgatcggcgc agggattgcc gaaccgtgac tcggcggcaa gtattgttgc
      781 gcaaggaaat ttttacggca ctgaccgttc tgcggaagga ataagattat agaattttac
      841 tcagacataa aaaaaacccg gcatagggga ccgggaagag gatagtctgc cgtctccaga
      901 ctaataaacc gttataacac tccctgttgg cacgggaaac tttgtgctct cagtaagtta
      961 aatataactt ttactggaaa taagatcagc cattttttta taaacataag ctatacgctg
     1021 tgcgaaaata tattcgtgct gcatttactt attatcaatt aactgttatg caaaactact
     1081 ttgtggataa attttggtcc taccaaatct ggcagttttt tcgctaagaa acagtctggc
     1141 aacatttcat tagtatactg aaattgaaat aatcgcagta tgaaatataa gggataatca
     1201 tgactcatgt ctgctcggtg atcctcattc gtcgttcatt cgatatttat catgaacagc
     1261 ataaaatatc gctgcataac gagagtatcg tgctgctgga gaaaaatttg gcagacgatt
     1321 ttgcgttttg ttcaccggat acgcgacgac tggatatcga tgagctgaca gtttgccatt
     1381 acttacaaaa tattcgtcag ctaccacgca atttagggtt acatagcaaa gaccgtctat
     1441 taattaacca gtcacccccc atgccgctgg tgacggcgat ttttgatagc ttcaatgaat
     1501 ccggggtaaa ttcaccgata ctgagcaata tgctctacct ttcctgttta tcgatgtttt
     1561 ctcataagaa agaactgatc cccttacttt tcaatagcat cagcactgtt tcaggaaaag
     1621 ttgaacgcct tattagcttt gatatcgcca aacgttggta tctgcgcgat atcgcggaaa
     1681 gaatgtatac cagcgagagt ctcataaaaa aaaagttgca ggatgaaaat acctgtttca
     1741 gtaaaatatt actcgcttcc aggatgtcga tggccagacg attactcgag ttacgtcaaa
     1801 ttcctctgca tactattgca gaaaaatgtg gctatagcag tacgtcgtac tttataaaca
     1861 catttcgaca atattatggt gtaacgccac atcagtttgc gcaacattcg ccaggtacct
     1921 tttcctgaca tattttgcat ttgaatattg gtcaggatct cacacctgct tcatgtgaaa
     1981 ctcttccctg atgatttctg ccgggctacc ggctagttct ctttcgcagt ttgccaggcg
     2041 gtataggtat tttcaggagg aaaagactcg gcatgtttgg gattattaag ctgacaattc
     2101 atacgattac gggaatgtgg gtcagtattg tgctgttcaa attgatgacg aatggctgga
     2161 gtggtttcta ctttcagtgc tgtgtactaa gcctggtatt cctgacggtt agctggttgt
     2221 tgagtggtga atggttagca ggaaagagta aggctgaacc ttcacgttca accttactct
     2281 catttacacg ttacgctttt ttaaagcggg caaagagatg ttccactaca acgaaaaaga
     2341 ccggaacgaa gtaaattgcc agcactgttg cggcaaacat cccgcccatc acgccggtac
     2401 ctaccgcgtt ttgcgcgcca gaaccggcac catgactgat aaccagcggc agcacgccga
     2461 gaataaaggc gagagaggtc atcaggattg ggcgtaaacg catccgcgcc gcttcgatga
     2521 ttgcctctat cggcgttttc ccttctttct gcatcatctc aacggcaaat tcgacaatca
     2581 ggatggcgtt cttggcggaa agcccgatgg tggtcagcaa accaacctgg aagtagacgt
     2641 cattacttaa gccgcgcaga tcggtggcca gtaatgcgcc aacgacgcct aacggaacaa
     2701 ccaacatcac cgagaacgga attgaccagc tctcatagag tgcggcgagg gcgaggaaca
     2761 ccacgaccag tgaaatggca tacagtgcag gagcctggtt tgaggataac gcttcctgat
     2821 acgacagtcc ggtccatgag tagccgacgc ctgccggaag tttagcgacc aggtctgcca
     2881 taaatttcat agcgtcaccg gtacttttcc cggccgccgc ttcacctaaa atctccattg
     2941 acgggatgcc gttgtagcgt tccaatcgcg gtgaaccata ggtccattcg gtagacgagt
     3001 aggcagaaag cggtgccatc gtgccagagg cgttgcgtac ataccattgg ttgatgttat
     3061 ccggcaacat acggaacggc gtgcctgcct ggacatacac ttttttcacc cgcccctggt
     3121 tgaggaagtc gttcacgtag ctgctgccga aggcggtgga aattgtctgg ttgatatcag
     3181 acagcgccac acccattgcc tcagctttcg cggcgttgac gttcactttg aacatcggcg
     3241 tatcttccag gccgttcggg cgtaccccgg tgacctggtc aggcgattgc gctgccagtg
     3301 ataacagctc gtttcgtgcc tgggttagtt tttcgtgccc caggttaccg ttgtccagca
     3361 gttccatatc aaaacctgac gcggtaccca gttcagccac cgcgggtaag ttgaacggga
     3421 agacgacggc tttattgata ctgcttaacg caatcattgc ccgctgaatg atcgcggtaa
     3481 ccgagttttc ctcaccgaca cgttcagacc acggcttgag actgataaac gccaggccgt
     3541 tgttttgccc ctggccgctg aagccaaagc cgccaacggt aaacaccgac tgaacattat
     3601 ctttctcttt tgtaagataa taatccgtca cctgttgcag cactttcgtg gtgttaacca
     3661 tggtggcacc ggaaggtaac tgcgcggtgg tcataaatac cccctgatcc tcttctggta
     3721 agaaagaggt cggtgtacgc aggaacagca ccgccatccc ggcgcaaatc agcaggtaga
     3781 ccaccatgta gcgaccggta caacgcaaca gcgagcgggt gctatcggta tagtgctgag
     3841 ttgatttttc aaacagcgtg ttgaagcgtg cgaacagggc gttaggtttg tgaccgcctt
     3901 ccggcgcggc tttcagaatg gtggcgcaca gggcaggggt caggctcatt gccacaaata
     3961 ctgaaagcag catggaggag atcagcgtga tggagaactg gcggtagatc tccccggttg
     4021 cgccgctcat aaaggccatc ggcataaaca ctgcggaaag aacaacagca ataccgacca
     4081 gcgcacgttg gatctgcccc atcgatttat gcgtcgcttc cttcggtggt agcttatctt
     4141 ccgcaatgac acgctcgacg ttctccacca ccacgatggc gtcatccacc agtaacccta
     4201 tcgccagcac catcccgaac atagttaacg tgttgatggt gaaaccgacc gccgacaaga
     4261 tcgcaaacgt cccgagaata accaccggta cggcaatcgt cgggatgatt gtggcacgga
     4321 aattctgcaa aaacagatac atgaccagga agacgaggat gatagcctca accagtgttt
     4381 taaaaacttc ctgaatagaa atttcgataa acggcgtggt gtcgtaagga taaaccgtct
     4441 tcagacttgc ggggaaatag gctgataagc ggttcagctc ctctttgact gcccgcgagg
     4501 tatccagggc gtttgctccg gtagccagtt tgatggcgat cccggcagca ggtttgccgt
     4561 tatagcgcgc cacggtggaa taatcttccg ctccaagttc gacgcgggcg acatcacgca
     4621 gcagcacttg cgaaccatct tgctgaactt tcaacaggat tttgccaaat tcttccggcg
     4681 tttgcagacg cgtctgcaca atgatcgagg cgtttagttg ctggtctgcc gcctgtggca
     4741 tgccacccag ttgaccaccg gaaatctggt tgttttgcac cttaatctgg gaaataacat
     4801 cggaaggtac cagattgtat ttattgagtt tttgcgggtc cagccagata cgcatggcgt
     4861 actcggaacc aaagagctgt acgctaccaa cgcccgcggt acggcttagc gggtctttga
     4921 tattagacgc tacatagtcc gcgatatcgt actggttgag gctgccgtta tcagaaataa
     4981 acgccgctac catcagaata ttactgctcg acttatcaac gctaatcccc tgctgctgca
     5041 ccgcttcagg taatgaaggc atagcgagtt gcagtttatt ttgcacctga acctgtgcga
     5101 tatcaggaga tgtcccagtc tcgaaggtca gagtgataga ggcattaccc gccgcatcac
     5161 tggttgaaga catgtacatc aggccatcaa gcccattcat attttgctca atcacctgag
     5221 tgaccgagtc ttctaccgtt tgcgcatcgg cacctggata ggtagcgctg acggtaatgg
     5281 tcggtggcgc aatctgcgga tactgcgcaa ccggtaagtt catgatcgcc agaccacctg
     5341 caagcatcat aataatggca agtacccagg caaaaaccgg gcgatcaata aaatagttag
     5401 ccatgaaagt ccccttaagc ctgcaacgtt attgtttcga ttcggtgctg gcgttttcct
     5461 ggctggagga aattgctcgt gctttgatac ccggacgaat gcgttgcaaa ccggaaacga
     5521 tcacccgatc gccagcctgc aagccagagg tgacaaccca ctggtcgccg atggctttgc
     5581 tggcttcaat ttcgcgtagc tgcacaacat cgtctttatc cagaatgagc gccgttgctt
     5641 taccctgggc gttgtgagtg acgccttcct gcggcaccag taatacattc tggcggctac
     5701 cttcatccac taatgccgtg acgtacatgc caggcagcaa gtcaccattt gggttgggga
     5761 aaatcgcccg taacgtcacg gagcccgtgg tttcatccac cgtcgggtcg gagaatttca
     5821 gtgtgccggt ctggctgtag cgtttaccat tttccagatt gagctgtact ggcgtactgc
     5881 cctgaacctg tttgatttgc ccactggcga cctcttcttt catgcgcaag aaatcctgca
     5941 ccgactgcgt gagatcgaca taaatcgggt ccagacgttg tacggtaacc agcgaatctg
     6001 cctgattagc ggtaacgagt gcgccgacgg tcaccgacga tttcccgctg acgcccgtaa
     6061 tcggcgaggt gacattcgcg tattgcagat tgatcgtcgc ctgttcaaca gccgctttgg
     6121 cgacggtgac attggcttct gcttcattca gctgggtgcg cgcggtgtcg taatcctgac
     6181 ggctaacgta gttggtcttc agcaacgatg cctggcggtt aaaggtgatg cgggcattgc
     6241 tggcggtaga gagcgctttc gccagcgagc ctttggcgga gtttagctcg gcctgtaaag
     6301 gtgcaggatc aatctgatac agcgaatcgc cctggttcac tttatcgcct tcgataaagt
     6361 tgcgtttaat gataataccg cccacctggg gacgtatctc ggcaacttca taaggaacgg
     6421 ttctaccggg caattcgctc aacacattga ccgaaccggg ggagagtgtg acgacaccga
     6481 cctcaggcgt catggcggcg gcgttttccg ccgatttgtc atcgcaggcg gtgagcatcg
     6541 cgccgcagaa taacaacggt attaacagct ttcttcttct gttcatttta gtccctgaaa
     6601 attcttgaga aataacggtt ttttaaattg cgcgcgagga gagatatgag ggatttacag
     6661 accagcggca tagtggggtt gtcagccagt aaggtgggat acaggcacag tgatcgacat
     6721 ggtgaggtca acgacaaaaa aaatgttgcc gttctgccaa cagttcctgc cagcattcgg
     6781 gcaacgaagg gttctactgg tggatacaca tacctgggga ataaagtcgt tttagcctct
     6841 cctttagtaa tcaccggggg taatgaaatc agcatctgca tccctcgtca tgccagccat
     6901 caatttcagt tgcttatgtc ctgactaaaa ataagatgtg atacccaggg tgacgatgtc
     6961 gctcatacgt cttagtttca gcttgtacat aatgttgaat ttctgaatct ttaatgtttg
     7021 ttgcgaaata ttgagcgtcg acgtgatatt gctttcattt ttatttttca gaatcagttg
     7081 cataattttg aactgattat gtgaaaaaca taattgtgaa ttcttatggg gcaagtgttt
     7141 accataagta atctcaagaa gatttttgat actttctttg cggctaacaa aataaatagg
     7201 cgctttagct tttagtttac tgatgtgata aggtgccaaa acaataattc tctcggcatc
     7261 taatttctcc agaaatttaa tcgcttcttc atcaaggata tgattgtgat aggtatcaat
     7321 gataaccttg aagggtttat tgcctacttt tttgatctct gacagggaat tgatttttat
     7381 catttcgtga ttatctttca actgccaaaa gccctgtaaa agaaaagaat ctttcgtcat
     7441 gagaaaaatc ataacttgct ccttagccgt tatcgtttcg aacatgtcat ccttttcatt
     7501 atttacatcc ttgtccgaat cgttgttcaa tatagtaaac gcctataaga actttaccct
     7561 tcgtgaacta atacgtctat taacactaat caatacttca gatttatcca tcacgcactc
     7621 catatattga tcctaagtaa gattcctggt tgttatcagc ttgtaaatag taaacaaaaa
     7681 gttaacgttt gcggattact gcccaagaat aagttatcaa taaccagttt gtgatctctg
     7741 aagaatatta ctaaagttaa aatgtaatcc gatttaaata tcgagtctcc ttgtttcgac
     7801 ttaagctggc aattggattg ccagctttct tttaacgcat tgaaattacc tgagtaaata
     7861 acgcattcat gtcttcaggt ttttatatag cgtctggaga cacggatatt tatgcaatgt
     7921 aatcaacgcc agttgcaaaa aaatgaaccc ggattactta tttaaccgcc catagcatca
     7981 acgcttacgg aactctttgt tcactttccc acagcacttt taaaacatta aacctgacta
     8041 cggaaaatat cagccatgat aatgtttgaa atggctaatt tgccatggag tgaaaaaaat
     8101 tagatgaaat tcagtaggtt gaaataatca ctagcaggta attatttcaa tgatagtgcg
     8161 caattgatct acaacactcc gtagcggaga gagtattaat cggatcatag tcatatcaag
     8221 tgactatgat ccgggtgaca accgggataa ttattgctgc ttaacgaaca aactggcgaa
     8281 gctgaacagg ctggcggcgc taaatatcag ttcaattccc accagtgtgg aaaccagcgt
     8341 tacagacacc atcggcgttg caccaaggaa tatccaggca atgacgatat ccagcacacc
     8401 aataacgagc tgtagccagc tgcctttcat tgaacgctga cgataccaac tcatcaggcg
     8461 aataacccct gcaacacaga acaaaccggc aataaatgcc gcaatggcaa aaatgcccag
     8521 ctccggtgcg cggatgaaga aatagccgat caataaatag gcgactgcga cgaggaaacc
     8581 ggataatacc ggccagaaat tatgactgcg gttgctgaat aacccgacaa taagcgcaat
     8641 gcccgagcag attaataatg cacccactac tgtgcttaaa atatcgccag agacgaacgg
     8701 gaaactgata cacagcaacc cgacgataaa cagtagcacg gcaataaact ggattgctct
     8761 gcgatgtttt ttaagcatct ccagatcaaa cttcaaaatt gttgccttat ctatatataa
     8821 catagaacca ccctataaaa ttaagaagaa aatcccctgc tatcaatcta tgccaaaaac
     8881 gcgtctaaga atgtagtcga tttaataaaa atttcctaat tacagtatct gatgc
//
LOCUS       DAZPAC010000166         8882 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZPAC010000166 DAZPAC010000000
VERSION     DAZPAC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8882
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.166"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            5..1729
                     /gene="iucA"
                     /locus_tag="ACHUPM_003251"
     CDS             5..1729
                     /gene="iucA"
                     /locus_tag="ACHUPM_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR0870946.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            1730..2677
                     /gene="iucB"
                     /locus_tag="ACHUPM_003252"
     CDS             1730..2677
                     /gene="iucB"
                     /locus_tag="ACHUPM_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR0870947.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            2677..4419
                     /gene="iucC"
                     /locus_tag="ACHUPM_003253"
     CDS             2677..4419
                     /gene="iucC"
                     /locus_tag="ACHUPM_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR0870948.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            4416..5753
                     /gene="iucD"
                     /locus_tag="ACHUPM_003254"
     CDS             4416..5753
                     /gene="iucD"
                     /locus_tag="ACHUPM_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR0870949.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            5759..7954
                     /gene="iutA"
                     /locus_tag="ACHUPM_003255"
     CDS             5759..7954
                     /gene="iutA"
                     /locus_tag="ACHUPM_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR0870950.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDFLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(8505..8648)
                     /locus_tag="ACHUPM_003256"
     CDS             complement(8505..8648)
                     /locus_tag="ACHUPM_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870951.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2138 a         2252 c         2346 g         2146 t
ORIGIN      
        1 gactatgacc ctgccctctg aaaaaccagc cacagatgtg gctgcgcagt gcttcctgaa
       61 tgcactgatt cgtgaaacca cagactggaa actgacagaa tacccgccag acgaattgct
      121 catcccgctg gatgagcaga aatcgctcca tttcagagtg gcttatttct ccccaaccca
      181 gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca tcaggcagtt atcctgtcga
      241 ctttaccact ctctcccggc ttattattga taagctacgc catcaactgt ttctgcccgt
      301 tcccctctgc gaaactttcc accagcgcgt gctggaaagc catgtccata cgcaacaggc
      361 aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa gcgttgaatt ttggcgaggc
      421 tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg cctaagtctc atgaaccgtt
      481 taaccgacgg gaggctgaac gctacctgcc tgatatggca ccccacttcc cactgcggtg
      541 gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg catctcaacc ttcaacagcg
      601 gctgacgcga tttgccgcag agaatgcgcc tcagttactc aacgaattaa gtgacaacca
      661 atggctgttc ccgttgcacc cgtggcaggg ggaatatctt ttgcagcagg ggtggtgcca
      721 ggcacttgtt gctaaagggc tgattaaaga cttaggtgag gccggcacgt cgtggctgcc
      781 gaccacctct tcccgttccc tctactgtgc caccagccgc gatatgatca agttctccct
      841 gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg aaagaagtga agcgtgggat
      901 gcgcctggca cgcctggctc aaaccgacgg ctggcagatg ctacaggtcc gcttcccgac
      961 tttccgggta atgcaggagg atggctgggc cgggctgctc gatcttaacg gcaacatcat
     1021 gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg gatcagccga aaagccagac
     1081 taacgtactg gtctccctga ctcaggccgc acctgatggc ggtgattcgc tgctggtttc
     1141 ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg caacaggccg cccatgcatg
     1201 ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt acggctgaag cggattacgg
     1261 cctggtgctg ctggcgcatc agcaaaatat tcttgtccag atgcttgggg atctgccggt
     1321 cggatttatt taccgtgact gtcagggcag cgcttttatg cctcacgcga cagactggct
     1381 cgattctatt ggcgaggcgc aggcggaaaa tattttcacc catgagcagt tgctgcgcta
     1441 tttcccttat tacctgctgg ttaactccac ttttgctgtg accgctgcgc tgggggctgc
     1501 cgggctggac agcgaatcga atctgatggc tcgtgtacga gcatcgctgg ctgaagtgcg
     1561 tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg gaaagtccgt actggaacgt
     1621 aaaaggtaac tttttctgtt atctgaacga tcataacgag aacaccattg ttgacccttc
     1681 ggtgatctat ttcgatttcg ctaacccgct gcaggctcag gaggtctgaa tgtctgaggc
     1741 aaacattatt cacagcagat atggactgcg ctgtgaaaaa ctcgacaagc ccctgaatct
     1801 tggctgggga ctggacaata gcgcggtgtt gcactgtccc ggggagctgc cgacagggtg
     1861 gctgtgcgat gcgctggatc agatatttat cgccgcacca caactgtcag cagtggctct
     1921 cccctgggcc gaatggcgtg aggagccaca ggcgctgacg ctttttgggc aggtaaaaag
     1981 cgacattatc catcgtactg ccttctggca gttaccgtta tggttgagtt caccggcaaa
     2041 ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag atttatttcc cgcagcgccc
     2101 accccgtcca cagggtgagg tttaccgccg ttacgatcca cgtattcgca ggatgctgag
     2161 tttccgcatt gccgatcccg tttctgatgc agaacgtttc acccgctgga tgaatgatcc
     2221 gcgcgttgag tatttctggg agcaaagtgg ctcactggag gtacagaccg cctatctgga
     2281 gcgccagctc accggtaaac atgcattccc gctgattggg tgcttcgacg atcggccatt
     2341 cagctatttc gaaatctact gggcggcgga agaccgcatt ggtcgtcact attcgtggca
     2401 gccctttgac cgtggcctgc atctgctggt tggtgaacag caatggcgcg gggcccacta
     2461 tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg ctggatgagc cccgtacgca
     2521 gcgcacagta ctggagccac gcaccgataa ccagcgcctg ttccgccatc ttgagcctgc
     2581 gggataccgg acaattaaag agttcgactt cccacataag cgctcgcgca tggtgatggc
     2641 ggatcgtcat cacttcttca cggaggtcgg tctgtaatga atcacaagga ctgggatttt
     2701 gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg agtatgagca ggttttccac
     2761 gcggaatctc agggcgatga ccactactgc attaacctgc cgggagcaca atggcgcttc
     2821 atcgctgaac gtggtatctg gggctggctg tggattgatg cccaaacgtt gcgctgcacg
     2881 gacgaaccag tactggcgca gacgctgctg atgcagctaa agccggtgct gtcaatgagt
     2941 gatgccactg ttgcagagca tatgcaggat ttgtatgcca cgctactggg cgacctgcaa
     3001 ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga ttaatcttga tgccgatcgt
     3061 ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta ataaaggtcg ccgtggctgg
     3121 ggcaaagagg cgctggaacg atatgcgcca gagtatacca acaccttcag actgcactgg
     3181 ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata acgatctgga tattcagcag
     3241 ttgttgactg ccgcaatgga tccgcaggag tttacacgct tcagtcaggt ctggcaggaa
     3301 aacgggctgg atcacaactg gctaccgctg ccggtacatc cgtggcagtg gcagcaaaaa
     3361 atcgccacag acttcatcgc tgattttgtc gaaggcagga tggtgtctct cggcgagttt
     3421 ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga ccaacgccag ccgccgggga
     3481 gggctggata tcaagctgcc gctgaccatc tacaacacct catgctaccg gggaatccct
     3541 ggcagatata tcgctgccgg accactggct tcacgctggc tgcaacaggt ttttgcgacc
     3601 gacgccaccc tggtgcaaag cggcgcagtg atccttggtg aaccggctgc aggctatgtg
     3661 tcccatgaag gctatgccgc gcttgcccgg gctccctatc gctaccagga aatgcttggt
     3721 gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg atgaaagtcc tgttctgatg
     3781 gcaacactga tggagtgcga cgaaaacaat cagccgctgg caggcgcata tatcgaccgc
     3841 tccggactgg acgctgaaac ctggcttacg caattgttcc gggtggttgt ggttccgctg
     3901 tatcacctgc tttgccgcta cggtgtcgcg cttattgcac acggacaaaa tataacgctc
     3961 gccatgaaag agggggtacc acagcgtgtt ctgctgaaag acttccaggg cgatatgcgg
     4021 ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc aggaggtgcg tgatgccaca
     4081 tcccgtctga gtgcggacta cttaatccat gatttgcaga cgggtcactt cgtgacagta
     4141 ctgcgtttta tttcgccact gatggttcgt cttggcgtac ctgaaaggcg attttatcaa
     4201 ctgctggcag cagtgttgag tgattacatg aacaaacatc cacaaatggc agagcgtttt
     4261 gcgcttttct cactcttcag gccacaaatc attcgcgtgg tgctgaaccc ggtaaaactg
     4321 acctggccgg acctggatgg cggcagtcgc atgctgccga attaccttga gaatctgcaa
     4381 aatccgctgt ggctggtaac tcaggaatat gaatcatgaa aaaaagtgtc gattttattg
     4441 gtgtcgggac agggccattt aatctcagca ttgctgcatt gtcacatcag atcgaagaac
     4501 tgaactgtct cttctttgac gaacatcctc atttttcctg gcatccgggt atgctggtac
     4561 cggattgtca tatgcagacc gtctttctga aagatctggt cagtgcagtt gcacctacaa
     4621 atccctacag ctttgttaac tatctggtga agcacaaaaa gttctatcgc ttccttacaa
     4681 gcaggctacg tacagtatcc cgtgaagagt tttctgacta tctccgctgg gctgctgaag
     4741 atatgaataa cctgtatttc agtcataccg ttgaaaatat tgatttcgac aaaaaaagtc
     4801 gattgtttct ggttcagacc agtcggggag aatattttgc ccgcaatatc tgccttggta
     4861 caggaaaaca accttattta ccaccctgtg tgaagcatgt gacacaatcc tgtttccatg
     4921 ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa acggataacc gtggttggtg
     4981 gaggacagag tggtgcagac ctgttcctta atgcattacg cggggaatgg ggagaagcgg
     5041 cggaaataaa ctgggtctca cggcgtaata attttaacgc actggatgag gctgcttttg
     5101 ctgatgagta ttttacacct gaatatattt caggcttctc cggactgaag gaagatattc
     5161 gccatcagtt actggatgag cagaaaatga catcggatgg catcactgcc gattctttac
     5221 tgaccattta tcgtgagttg taccaccgtt ttgaagttct gagaaaacca agaaatatcc
     5281 gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag tggtccgggc tggaggttat
     5341 tgatggaaca tcatctggat cagggcagag agagcctgga aagtgatgtg gtgattttcg
     5401 ccacaggtta ccgttctgca ttgccacaaa tacttccctc actgatgccc ttgatcacca
     5461 tgcacgataa gaacaccttt aaagtgcgtg atgacttcac tctggaatgg agtggcccga
     5521 aagaaaataa catctttgcg gtcaacgcca gcatgcaaac tcatggcatc gccgaacccc
     5581 agctcagcct gatggcctgg cgatctgcac gtattcttaa tcgcgtactg gggcgtgatt
     5641 tattcgatct cagtatgccg cccgcactga ttcagtggcg cagcggcagc cggaaaaaac
     5701 cgcagccgga ggctgcttct ttaactcact atacagcaaa tattcaggaa taacgatgat
     5761 gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt cttgtcgtgc tgtcaccagc
     5821 tgtcgcccag caaaacgatg ataatgagat catagtgtct gccagccgca gcaatcgaac
     5881 tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat gccgaactgg agcagcagat
     5941 tcagggcggt aaagagctga aagacgcact ggctcagtta atccccggcc ttgatgtcag
     6001 cagccagagt cgaaccaact acggtatgaa catgcgtggc cgcccgctgg ttgtcctgat
     6061 tgacggtgtg cgcctcaact cttcacgttc cgacagccga caactggact ctgtcgatcc
     6121 ttttaatatc gaccatattg aagtgatctc cggcgcgacg gccctgtacg gtggcgggag
     6181 taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag ccggaaacca tgatggagtt
     6241 tgaggctggc acaaaaagtg gctttaacag cagtaaagat cacgatgagc gcattgccgg
     6301 tgctgtctcc ggcggaaatg accatatctc cggacgtctt tccgtggcat atcagaaatt
     6361 tggcggctgg tttgacggta acggcgatgc caccctgctt gataacaccc agaccggcct
     6421 gcagcactcc aatcggctgg acatcatggg aaccggtacg ctgaacatcg atgaatcccg
     6481 gcagcttcaa ctgataacgc agtactataa aagtcaggga gacgacaatt acgggcttaa
     6541 tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca ccatacgtca gtaaggggct
     6601 gaattctgac cgcattcccg gcactgagcg gcatttgatc agcctgcagt actctgacag
     6661 tgatttcctg ggacaggaac tggtcggtca ggtttactac cgcgatgagt cgttgcggta
     6721 ctacccgttc ccgacggtaa atgcgaataa acaggcgaca gctttctcct cgtcacagca
     6781 ggataccgac cagtacggca tgaaactgac tctgaacagc caactgatgg acggctggca
     6841 aatcacctgg gggctggatg ctgagcatga gcgctttacc tccaaccaga tgttcttcga
     6901 tctggctcag gcaagtgctt ccggagggct gaacaaccat aagatttaca ccaccgggcg
     6961 ctatccgtca tatgacatca ccaatctggc ggccttcctg caatccagct atgacattaa
     7021 tgatattttt accgttagcg gtggcgtacg ctatcagtat actgagaaca gggtagatga
     7081 tttcatcgac tacacgcagc aacagaagat tgctgccggg aaggcgatat ctgccgacgc
     7141 cattcctggt ggttcggtag attacgataa ctttctgttc aatgctggtc tgctgatgca
     7201 catcaccgaa cgtcagcagg catggttcaa tttttcccag ggggtggcat tgccggatcc
     7261 ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg aacggccatc tttccctgac
     7321 aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg aaagtcgatt cttatgaact
     7381 gggctggcgc tttaccggtg acaacctgcg gactcaaatc gcggcatatt actcgctttc
     7441 caataagagc gtggaaagga ataaagatct gaccatcagt gtgaaggacg acaggcgccg
     7501 tatttacggc gtggaaggtg cggtggactt cctgatcccg gatactgact ggagtaccgg
     7561 tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac ggtcaatggc aaaaatatga
     7621 cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt aactgggcgc cggaaccgtg
     7681 gagtctgcgt gtacagagca ccacttcttt cgacgtaagc gatgcagagg gtaacgatat
     7741 taatggttac actaccgtcg attttatcag tagttggcag cttccggtgg gaacactcag
     7801 cttcagcgtt gagaacctct tcgaccgtga ctataccact gtctggggac agcgtgcacc
     7861 tctgtactac tgcccgggtt acggccctgc ttcactgtac gactacaaag gccggggccg
     7921 aacctttggt ctgaactact cagtgctgtt ctgacgggga tgcttttgca acaagggtat
     7981 gctgtaaaca acatggtcct tggtggccat gttgtttaca gccagtataa tatacagcct
     8041 taacaagata ttccgtctcc ggaatggact gttcaccact gaataacatt atttctgttt
     8101 tatggatctt tgactgaaat ttatcgcata tcctcatttc attgtccgtc tgaaaactaa
     8161 ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc ctgaaattcc tgagaggatg
     8221 atctgcgccc aatatcctga gccggaactt tgctgcaatg aaggccaacg aaaaatgggg
     8281 gagtatgtta tagagtactt gctacagggt aaaaagaggt acctgtcgct ggttctccgt
     8341 tcagtttaac cgggagattt tctcctgcgc ctgtcataaa gtccggtaat ggagattgtt
     8401 aatacgatgg tgcacgatgc attgtcggtg cccggcccgg aggatgcccc gtaactacat
     8461 acggatcagg gttggcaata tcgaatggca ggctataggc aaagttaaag ccccatggag
     8521 tagcacaaaa tatgccacac aaaggaaacg gtctgaataa cgcagtgatg gagaacttct
     8581 tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt cgccgtgaat cactgaacga
     8641 tgttccatgc gaacagatat tcgatcatat cgaaatatta aacattaccc ctccccgcat
     8701 tgggcaaaaa aaaaacaaaa aacgatcaat tagtattgat tcacataatc tctcatcatt
     8761 aatgagaaca ttgtcacata tttacaatat agaactgtta cttttagtaa gctgttagta
     8821 accaactgtt actaataatt ttaattctta atactttgtt cattcaataa atgagttgag
     8881 ag
//
LOCUS       DAZPAC010000167         8745 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZPAC010000167 DAZPAC010000000
VERSION     DAZPAC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8745
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.167"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..115
                     /locus_tag="ACHUPM_003257"
     CDS             <1..115
                     /locus_tag="ACHUPM_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFR0870952.1"
                     /translation="TIPVITKFDSFRRMFVCIMLSAIWSGVMWFFIISLVK"
     gene            complement(96..431)
                     /locus_tag="ACHUPM_003258"
     CDS             complement(96..431)
                     /locus_tag="ACHUPM_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFR0870953.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYIYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            512..796
                     /gene="traQ"
                     /locus_tag="ACHUPM_003259"
     CDS             512..796
                     /gene="traQ"
                     /locus_tag="ACHUPM_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFR0870954.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            783..1328
                     /gene="trbB"
                     /locus_tag="ACHUPM_003260"
     CDS             783..1328
                     /gene="trbB"
                     /locus_tag="ACHUPM_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFR0870955.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            1258..1605
                     /gene="trbJ"
                     /locus_tag="ACHUPM_003261"
     CDS             1258..1605
                     /gene="trbJ"
                     /locus_tag="ACHUPM_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFR0870956.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            1586..1978
                     /gene="trbF"
                     /locus_tag="ACHUPM_003262"
     CDS             1586..1978
                     /gene="trbF"
                     /locus_tag="ACHUPM_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFR0870957.1"
                     /translation="MKEKINDTEPGIKQIEREIERGCDNAKKYFWLFVVFFAAGLIVR
                     NVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLAAASLSPLCEII
                     FGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            1965..3338
                     /gene="traH"
                     /locus_tag="ACHUPM_003263"
     CDS             1965..3338
                     /gene="traH"
                     /locus_tag="ACHUPM_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFR0870958.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMNYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            3335..6160
                     /gene="traG"
                     /locus_tag="ACHUPM_003264"
     CDS             3335..6160
                     /gene="traG"
                     /locus_tag="ACHUPM_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFR0870959.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAERRRAMAWFFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            6157..6666
                     /gene="traS"
                     /locus_tag="ACHUPM_003265"
     CDS             6157..6666
                     /gene="traS"
                     /locus_tag="ACHUPM_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFR0870960.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITLFVIISFIYTIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            6680..7411
                     /gene="traT"
                     /locus_tag="ACHUPM_003266"
     CDS             6680..7411
                     /gene="traT"
                     /locus_tag="ACHUPM_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFR0870961.1"
                     /translation="MKTKKMMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDAAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            7614..8351
                     /locus_tag="ACHUPM_003267"
     CDS             7614..8351
                     /locus_tag="ACHUPM_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870962.1"
                     /translation="MTSDNIAEYIGENNQFTEKPATEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            8402..>8745
                     /locus_tag="ACHUPM_003268"
     CDS             8402..>8745
                     /locus_tag="ACHUPM_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraD N-terminal domain-containing protein"
                     /protein_id="HFR0870963.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLA"
BASE COUNT         2273 a         1943 c         2262 g         2267 t
ORIGIN      
        1 taccataccg gttatcacga agtttgacag tttcagaagg atgtttgttt gtattatgtt
       61 gtcagccata tggtctggag tgatgtggtt ttttattatt tcattagtga aatgactggt
      121 gctaatttgt cataaatgcc aaatccaata gtgaggttga ttaccacaga ccagaggata
      181 aaccagactg ctatccagtg acttctgaat aactcacaaa aataaatata gatgattaga
      241 aatagccata tcacaacagc gataatcgct ataaataaca gtatcgtcac aaataaatca
      301 gcaaaggttt taatggggag ggagtgcaat aatgaataca ccagtaaagc aaaagccgca
      361 ttacctgata atgactctct tatgatgttt ctcactattt ccagtttttc tttaaaagat
      421 aactttttca tttgtacctc tgattaatat cacacaaacg cgaatattac agacatgaat
      481 actctcatgt aaataataac cggagtttaa catgataagt aaacgcagat tctctttacc
      541 ccggcttgat attacgggaa tgtgggtatt ttccctgggt gtctggtttc atatcgtcgc
      601 ccgtcttgtt tacagcaaac cctggatggc tttttttctg gcggagttga ttgcggccat
      661 ccttgtgctg ttcggcgcat accaggtgct ggatgcatgg attgcacgtg tcagcctgga
      721 agagcgtgag gcactggaag ccaggcaaca ggccatgatg gaagggcagc aggagggcgg
      781 acatgtctct cactaaatta ctgccggtca ttttgttgat gatggccaca ggtgtgcagg
      841 cctccacccg ggatgaaatt gaacgactct ggaatccgca gggaatggcc gtgcagcctg
      901 cacaaccggc agcagacaca tcagccagaa cagaaaagcc ggctccccgc tggttccgcc
      961 tcagcaacgg caggcaggtt aacctggccg actggaaggt ggtgctgttt atgcaggggc
     1021 attgccctta ctgtcaccag tttgacccgg tactgaagca gctggcacag cagtacggct
     1081 tttccgtttt tccctacacc ctggatggtc agggggatac tgcctttccg gaagcattac
     1141 cggtgccacc ggacgtgatg cagaccttct tcccgaatat cccggtggcc acaccgacca
     1201 ccttcctggt caacgtcaac acacttgagg cattaccgct tttgcagggg gcaacggatg
     1261 ccgccagttt tatggcgcgg atggataccg ttctgcagat atacggagag gaaaaaggtg
     1321 cgaaataagc aggtggtgtt actcattgcc ggaatatccg gcatcgcgac gggaataatc
     1381 gtcagcctga atatcccctt tatccgtcag gggctttttt atcccgccag ccctgtggaa
     1441 attgtcgttt cgctgtgtct taccttttct gtttctgttg tgttttttgt gggggcaatt
     1501 gtgggatgga tttcggtgtc tgaaatatat tacagccgca tgacctgtct gcatgaatca
     1561 tcagaaatat cagagggaac ttatgatgaa agaaaaaata aatgatacag aaccgggaat
     1621 caaacaaatt gaaagggaga ttgaacgtgg ttgtgataat gcaaaaaaat acttctggct
     1681 ttttgtggtt ttctttgctg ccggattgat tgtaaggaac gttatgcatg attttttcag
     1741 tgccggtatt gattcgtgga aagctgaccc ggaattaaat aattttcgtt atatgtggaa
     1801 caccctgatg tatgtcattc cgattatgtt atatgcgctt gcagctggtt ttcttgctgc
     1861 ggcttctctg tcgccgttgt gcgaaataat atttgggggt gtccggatat tcctgctgaa
     1921 acgccgtatg cgccgggaga acacattaag ggagggcagc aataatgctt cgcattaaac
     1981 ctcttctggt tctttgtgcc gctttactga cggtcacacc cgcagcgtca gcggatgtga
     2041 acagcgatat gaatcagttc tttaacaagc tgggcttcgc ctccaatacc acacagccgg
     2101 gcgtctggca ggggcaggcc gccgggtatg cctatggtgg ctccctgtat gcccgcaccc
     2161 aggtcaaaaa tatccagctg atttccatga cgctgccgga tatcaatgcc ggatgtggcg
     2221 gcatcgacgc ctatctcggc tcgttcagtt ttattaatgg cgatcagttg cagcgttttg
     2281 ttaagcagat tatgagcaat gctgccggtt acttttttga ccttgccctg cagacaacgg
     2341 tgccggaaat aaaaaccgca aaagacttcc tgcagaaaat ggcaagtgac attaacagta
     2401 tgaacctcag ttcctgtcag gtggcacagg ggcttgtggg ggggctgttc cctcagacgc
     2461 aggaatctca gcagaaagtt tgtcaggaca ttggcagtga aagcaatatc ttcgccgact
     2521 gggccgcatc tcgtcagggg tgcacggtcg gagggcagtc ctcatctgtt cgtgacaaag
     2581 ccagcgacaa ggacaaggag cgggtgctga aaaaccagaa tatcatatgg gaggcgctga
     2641 aaaaaaacag aatgtttgac ggcaacaaag agttgaaaga atttatcatg acgctcaccg
     2701 gtacgctgat ttttggggaa gacagcgaga ttacccccct gccggcgcgt accactgacc
     2761 gcgatatcct ccgggcgatt atggaggggg gaacggcaaa aatttaccac tgcaacgatt
     2821 cggacaagtg tctgaaggtg gtggcggatg caaatgtgac catcgcccgg gataaagccc
     2881 tgaagtcaca gattacaaaa ctgctcacca gtattcagaa taaagcagtg agtgacactc
     2941 cgcttgacgc ccgggaacag gggtttattt ccagcaccac cattcccgtt tttaaatatc
     3001 tgattgaccc gcagatgctc ggtgtgtcca acagcgtcgt ttaccagctg acggactata
     3061 tcggctacga catcatgctc cagtacattc aggaactgct tcagcaggcc cgggccatga
     3121 ttgccaccgg gaactatccg caggctgtga tggacaatgt tctggagaat ctgaatcagg
     3181 cacagcaaca gattgccatt ttccagagtc aggtacaggt ccagcaggat gcgctgctgg
     3241 ttgtcgaacg tcagatgaac tacatgcgtc agcagctttc cgcccgtatg ctcagccgtt
     3301 accagaacaa ctatcacttc ggagggaatt cactgtgaat gaagtttatg tgattgccgg
     3361 tggtgagtgg ttgcggaata acctgaacgc cattgctgca tttatgagta cccggacctg
     3421 ggattcaatt gaaaaaatcg cgctcaccct gtctgttgtt gctgtggctt ttatgtgggt
     3481 acagcggcat aacgtgatgg atttgctggg ctgggtggcc gtgtttgtgc ttatcagcct
     3541 gctggttaat gtccgcacat cggtgcagat tattgataac agtgacctgg tcaaagtcca
     3601 tcgggtggat aatgttccgg tcggtctggc gatgccactt tctcttacga cccgtatcgg
     3661 gcatgcgatg gtggtcagct acgagatgat attcacccaa ccggacagcg tcacctacag
     3721 caagacaggc atgctgttcg gggcgaatct gattgtgaaa agcaccgatt tcctgtcccg
     3781 gaatccggaa atcatcaatc tgttccagga ctacgtccag aactgcgtgc tgggtgatat
     3841 ttacctgaat cataaataca cgctggaaga tctgatggcc tccgctgacc cctacacgct
     3901 gattttttcc cgacccagcc cgctacgggg cgtttatgac aacaataata atttcataac
     3961 ctgtaaggat gcgtcggtca cgctgaaaga caggctgaat ctcgatacaa agacgggagg
     4021 caagacctgg cattattatg tgcagcagat atttggcggc agaccggacc cggacctgtt
     4081 attcagacaa ctggtcagtg acagttacag ttatttctac ggctccagtc agtctgccag
     4141 ccagattatg cgccagaacg tcacaataaa tgccctgaaa gagggtatca ccagtaatgc
     4201 tgcccgtaat ggtgacaccg ccagcctggt gaatctggcc accacgtcat cgatggagaa
     4261 gcaacgtctg gcgcatgtct ctatcggtca tgtgacaatg cgaaacctgc caatggtaca
     4321 gactatcctg acgggaattg ccattggtat tttcccgctt ctggtactgg cagctgtctt
     4381 caacaaactg acactgtccg tcctgaaagg ctatgtgttt gccctgatgt ggctgcagac
     4441 ctggccgttg ttgtatgcca ttctgaacag tgccatgaca ttctatgcga agatgaatgg
     4501 tgcgccggtc gtgctgtctg aactctctca gatacagctg aaatactctg acctggcctc
     4561 tactgccggg tatctttcag ccatgatccc cccgttgtca tggatgatgg taaaaggcct
     4621 tggcgcaggt ttttccagcg tgtacagcca cttcgcctct tcctctatca gtccgacggc
     4681 cagtgcagca ggcagtgtgg ttgacggtaa ttactcctac ggcaacatgc agacggaaaa
     4741 cgtgaacggc ttcagctgga gcaccaacag caccacgtcg tttggtcaga tgatgtacca
     4801 gaccggcagt ggcgcaactg ccacacagac ccgtgacggt aatatggtga tggatgcaag
     4861 cggagcgatg tcccgtttac cggtcggtat caatgcaacg cgtcagattg cagcggcgca
     4921 acaggaaatg gcccgggagg cgtcgaacag agcagaaagt gccctgcatg gtttcagcag
     4981 cagtattgcc agtgcctgga acacgctcag tcagtttggt tctaaccggg ggagcagtga
     5041 ttctgtcacc ggtggcgctg acagcaccat gagtgcacag gactccatga tggccagccg
     5101 tatgcgcagt gcagtggaaa gctatgcgaa ggcgcataat atcagtaatg agcaggcgac
     5161 tcaggaactg gcatcaacag gttctaagtt ctcgtatggc ggatatgcag atgctcatgc
     5221 tgaatggggg attaaacctg gaattcttgg tgttggtggt ggggttggtg tcagaggcgg
     5281 gtttaaggga agtcttgatc gaacggatga tgattcacat tctgcaagta gtggcagcag
     5341 agctacccat gatgctcgcc atgatattga cgctaaagcc agtaaggact ttaaagaagc
     5401 cagcgattac tttaccagtc gcaaggtcag tgagtccggc agccatactg acaataatgc
     5461 tgactcccgg gtggaccagt tgtctgcagc cctgaactca gcgaaacaga gttacgacca
     5521 gtacacgacg aatatgaccc gcagccatga atatgcggaa atggcctccc gtactgaaag
     5581 catgagcggg cagatgagtg aagacctgtc gcaacagttt gcacagtatg tgatgaaacg
     5641 cgcaccgcag gatgcagaag ctattctgac aaataccagt tcgccggaga ttgcagaacg
     5701 tcgtcgggcg atggcctggt tttttgtgca ggaacaggta cagcctggtg ttgataatgc
     5761 ctggcgggaa tcccgtggcg atattggtaa aggaatggag agcgtacctt cgggtggtgg
     5821 cagtcaggat attattgctg accatcaggg acatcaggcc attattgagc aaagaacgca
     5881 ggacagtaat atccgtaatg atgtaaaaca tcaggttgat aatatggtca cagaatatcg
     5941 tggcaatatc ggagatactc agaacagtat ccgtggcgag gaaaatattg taagagggca
     6001 gtattctgaa ctgcaaaatc atcataaaac agaggcatta tctcagaata ataaatataa
     6061 tgaagagcga gaaagtcaaa agataatacc tgagccatca gaagaggctg tgaaaaaaat
     6121 ggttgatgat aaaaaggata gactgaaggg gccattatga tcactcacta tgatataaaa
     6181 acggagacac agaaactaaa ggatgtactc tctgtagagg gagttaatat tccgccatta
     6241 ttacaagtca taaaaccagg ggtgtatgtg tttttatggg ttttattatg gccgacattt
     6301 cttcgtttgc tggcagataa agttgatatc cgagatgctg gatttgatat atgttttagt
     6361 ggcgtaatgg gttttatcct ttttgttgcc attactaatg gaatgatgtt gtatcttgcc
     6421 atccctaaaa aattcagaga tgagtcgaaa gttatatctt ttatgtatga taagaacaaa
     6481 acttatatct tgtcgtttgt gattgttttt tcaatagttt cctttgctca tacgttctta
     6541 tttgggtttt tatcaataac tctgtttgta attatttcct ttatttatac tatagatata
     6601 aatcgttata atctctctgc aatagtctca gtaataggtc tgttcaaaaa agagtctgtt
     6661 agttaaaagg atattctgga tgaaaacaaa aaaaatgatg atggttgcac tggtcagttc
     6721 cactctggcc ctttcagggt gtggtgcgat gagcacagca atcaagaagc gtaaccttga
     6781 ggtgaagact cagatgagtg agaccatctg gcttaaaccc gccagcgaac gcacggtatt
     6841 tctgcagatc aaaaacacgt ctgataaaga catgagtggg ctgcagggca aaattgctga
     6901 tgctgtgaaa gcaaaaggat atcaggtggt gacttctccg gatgcagcct actactggat
     6961 tcaggcgaat gtgctgaagg ccgataagat ggatctgcgg gagtctcagg gatggctgaa
     7021 ccgtggttat gaaggcgcag ctgttggtgc agcgttaggt gccggtatta ccggctataa
     7081 ctcaaattct gccggtgcca cactcggtgt gggccttgct gctggtctgg tgggtatggc
     7141 tgcagatgcg atggtggaag atgtgaacta taccatgatc acggatgtgc agattgcaga
     7201 gcgtactaag gcaacggtga caacggataa tgttgccgcc ctgcgtcagg gcacatccgg
     7261 tgcgaaaatt cagaccagta ctgaaacagg taaccagcat aaataccaga cccgtgtggt
     7321 ctcaaatgcg aacaaggtta acctgaaatt tgaagaggcg aagcctgttc tcgaagacca
     7381 gctggccaaa tcaatcgcaa atattctctg aacctcgtca ggaggctgat ctgaccggcc
     7441 tccttgctga cgcctcgcgt tgctcgttgt ccaaccccgg gatgtaacaa aataaatttt
     7501 gtcacctccc ggcgtttccg aaataaatat gaaaacactg agcggttgtg caaattcttt
     7561 gtgcgaccgt gaagtgttgc cggcgaagct gggatcttcc ggagaaagtc attatgacat
     7621 cagacaatat cgctgaatat attggtgaga ataatcaatt tactgagaag cctgcaactg
     7681 aatacccccc ggtgatacag gataatacag tgaccactaa agactatctg tacagcaata
     7741 atattgatac tgacctttta gtggttatcg gagggcttat tgttatattg tttctcatga
     7801 tgcttatcat gtactttatt aattctctca ggcataagga attaactgaa atactgacaa
     7861 aaatatcttt tcctgaaaaa tccgggagca atttgcttaa accggaggcg gtcagctgta
     7921 atttgttgtc cctgtgtgtg ctggcaccgg tattctgtac acccagaaag ctggctgaag
     7981 tttccaggga gatcatcgtg gcgagtgcgc aggaaattgc atggacgccg gggagcagtc
     8041 gtattgatgg ccaccaggcg gagaatatca cctgggcgat ttcatcgcag tctcctgccg
     8101 ataagtatga tctcatcaat aaaacgtcct gtgtttctcc tgacagtggc cgtttttatg
     8161 aaccggacac agttctggat caccgtgaac gggtacgcct ttttattcag atccagtttc
     8221 cgcatgggag tgactggtct gaagtattgc gcctgactga cattgttaca gagaaggtct
     8281 cttccggcag gactgcaggc gggcatcacc accggcatac agggtacacc tggtctgttt
     8341 ctgtgaatta accccacatc atccttttct gccctgttcc agattttccc ggagtttcat
     8401 catgagtttt aacgcaaagg atatgaccca gggcggtcag attgcgtcca tgcgtatccg
     8461 catgttcagc cagatcgcca atatcatgct ttactgcctg tttatttttt tctggatcct
     8521 cattggtctg gtcttatggg taaaaataag ctggcagacg tttatcaacg gctgtattta
     8581 ctggtggtgt acgtcactgg aaggcatgcg ggatttaata aaatcccagc cggtctatga
     8641 gatccagtat tacggcaaaa ccttccggat gaacgctgct caggtactgc atgataaata
     8701 tatgatctgg tgcggagagc agctatggtc cgcattcgtt ctggc
//
LOCUS       DAZPAC010000168         8647 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZPAC010000168 DAZPAC010000000
VERSION     DAZPAC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.168"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(255..518)
                     /gene="rpsT"
                     /locus_tag="ACHUPM_003269"
     CDS             complement(255..518)
                     /gene="rpsT"
                     /locus_tag="ACHUPM_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR0870964.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            621..839
                     /locus_tag="ACHUPM_003270"
     CDS             621..839
                     /locus_tag="ACHUPM_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR0870965.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            847..1788
                     /gene="ribF"
                     /locus_tag="ACHUPM_003271"
     CDS             847..1788
                     /gene="ribF"
                     /locus_tag="ACHUPM_003271"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR0870966.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQKEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1831..4647
                     /gene="ileS"
                     /locus_tag="ACHUPM_003272"
     CDS             1831..4647
                     /gene="ileS"
                     /locus_tag="ACHUPM_003272"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR0870967.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4647..5141
                     /gene="lspA"
                     /locus_tag="ACHUPM_003273"
     CDS             4647..5141
                     /gene="lspA"
                     /locus_tag="ACHUPM_003273"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR0870968.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5266..5715
                     /gene="fkpB"
                     /locus_tag="ACHUPM_003274"
     CDS             5266..5715
                     /gene="fkpB"
                     /locus_tag="ACHUPM_003274"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0870969.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5717..6667
                     /gene="ispH"
                     /locus_tag="ACHUPM_003275"
                     /gene_synonym="lytB"
     CDS             5717..6667
                     /gene="ispH"
                     /locus_tag="ACHUPM_003275"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR0870970.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6733..7647
                     /gene="rihC"
                     /locus_tag="ACHUPM_003276"
     CDS             6733..7647
                     /gene="rihC"
                     /locus_tag="ACHUPM_003276"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR0870971.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7814..8635
                     /gene="dapB"
                     /locus_tag="ACHUPM_003277"
     CDS             7814..8635
                     /gene="dapB"
                     /locus_tag="ACHUPM_003277"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR0870972.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
BASE COUNT         1973 a         2161 c         2456 g         2057 t
ORIGIN      
        1 tatcacaata tccacattct ttgtagggga gtctggagat tgagtagata ttcttgttca
       61 gaatgtatca gccgatggtt ctacgattct taagccacga agagttcaga aagtgtaacg
      121 gcatgtctgc aaaagccagc atcttttgac tatctggcaa ccggtagtgt gttctctcac
      181 gcatcataaa agcagcaggc ataaaaaaaa cccgcttgcg cgggcttttt cacaaagctt
      241 cagcaaattg gcgattaagc cagtttgttg atctgtgcag tcaggttagc cttatgacgt
      301 gcagctttgt ttttgtggat cagaccttta gcagcctgac ggtccacgat cggttgcatt
      361 tcgttaaatg ctttctgtgc agcagctttg tcgccagctt cgatagctgc gtatactttc
      421 ttgatgaaag tacgcatcat agagcgacgg cttgcgttgt gcttacgagc cttttcagac
      481 tgaatggcgc gcttcttagc tgatttgata ttagccaagg tccaactccc aaatgtgttc
      541 tatatggaca attcaaaggc cgaggaatat gcccttttag ccttcttttg tcaatggatt
      601 tgtgcaaata agcgccgtta atgtgccggc actcgttacg tagtgatggc gcaggattct
      661 accagcttgc ggggtgtgaa tacagctttt ccgcgataaa aattgcagca ggcggtcact
      721 ttcttcccgt gatttgcgcc atggcaatga aaagccactt ctttctgatt tcggtactca
      781 atcgccggtt aaccttgacc gctgtacaag gtatactcgg acgattttca ctgttttgag
      841 ccagacatga agctgatacg cggcatacat aatctcagcc aggccccgca agaagggtgt
      901 gtgctgacta ttggtaattt cgacggcgtg catcgcggtc atcgcgcgct gttacagggc
      961 ttgcagaaag aagggcgcaa gcgcaactta ccggtgatgg tgatgctttt tgaacctcaa
     1021 ccactggaac tgtttgctac cgataaagcc ccggcaagac tgacccggct gcgggaaaaa
     1081 ctgcgttacc ttgcagagtg tggcgttgat tacgtgctgt gcgtgcgttt cgacaggcgt
     1141 ttcgcggcgt taaccgcgca aaatttcatc agcgatcttc tggtgaagca tttgcgcgta
     1201 aaatttcttg ccgtagggga tgatttccgc tttggcgctg gtcgtgaagg cgatttcttg
     1261 ttattacaga aagctagcat ggaatacggc ttcgatatca ctagtacgca aactttttgc
     1321 gaaggtggcg tgcgtattag cagcaccgct gtgcgtcagg cgctggcgga tgacaatctg
     1381 gctctggcag aaagtttact ggggcacccg tttgccatct ccgggcgtgt agtccacggt
     1441 gatgaattag ggcgcactat aggtttcccg acggcgaatg taccgctacg ccgtcaggtt
     1501 tccccggtga aaggggttta tgcggtagaa gtgctgggcc tcggtgaaaa gccgttaccc
     1561 ggcgtggcaa acatcggaac acgcccaacg gttgccggta ttcgccagca gctggaagtg
     1621 catttgttag atgttgcaat ggacctttac ggtcgccata tacaagtagt gctgcgtaaa
     1681 aaaatacgca atgagcagcg atttgcgtcg ctggacgaac tgaaagcgca gattgcgcgt
     1741 gatgaattaa ccgcccgcga attttttggg ctaacaaaac cggcttaagc ctgttatgta
     1801 atcaaaccga aacacggaac cgagaatctg atgagtgact ataaatcaac cctgaatttg
     1861 ccggaaacag ggttcccgat gcgtggcgat ctcgccaagc gcgaaccggg aatgctggcg
     1921 cgttggactg atgatgatct gtacggcatc atccgtgcgg ctaaaaaagg caaaaaaacc
     1981 ttcattctgc atgatggccc tccttatgcg aatggcagca ttcatattgg tcactcggtt
     2041 aacaagattc tgaaagacat tatcgtgaag tccaaagggc tttccggtta tgactcgccg
     2101 tatgtgcctg gctgggactg ccacggtctg ccgatcgagc tgaaagtaga gcaagaatac
     2161 ggtaagccgg gtgagaaatt caccgccgcc gagttccgcg ccaagtgccg cgaatacgcg
     2221 gcgacccagg ttgacggtca acgcaaagac tttatccgtc tgggcgtgct gggcgactgg
     2281 tcgcacccgt acctgaccat ggacttcaaa actgaagcca acatcatccg cgcgctgggc
     2341 aaaatcatcg gcaacggtca cctgcacaaa ggcgcgaagc cagttcactg gtgcgttgac
     2401 tgccgttctg cgctggcgga agcggaagtt gagtattacg acaaaacttc tccgtccatc
     2461 gacgttgctt ttcaggcggt cgatcaggat gcactgaaag caaaatttgc cgtaagcaac
     2521 gttaacggcc caatctcgct ggtgatctgg accactacgc cgtggactct gcctgcgaac
     2581 cgcgcaatct ctattgcacc tgatttcgac tatgcgctgg tgcagatcga cggtcaggcc
     2641 gtgattctgg cgaaagatct ggttgaaagc gtaatgcagc gtatcggcgt gaccgattac
     2701 accattctcg gcacggtaaa aggtgcggag cttgagttgc tgcgctttac ccatccgttt
     2761 atgggcttcg acgttccggc aatcctcggc gatcacgtta ccctagatgc cggtaccggt
     2821 gccgttcaca ccgcgcctgg ccacggcccg gacgactatg tgatcggtca gaaatacggc
     2881 ctggaaaccg ctaacccggt tggcccggac ggcacttatc tgccgggcac ttatccgacg
     2941 ctggatggcg tgaacgtctt caaagcgaac gacatcgtcg ttgcgctgct gcaggaaaaa
     3001 ggcgcgctgc tgcacgttga gaaaatgcag cacagctatc cgtgctgctg gcgtcacaaa
     3061 acgccgatca tcttccgcgc gacgccgcag tggttcgtca gcatggatca gaaaggtctg
     3121 cgtgcgcagt cactgaaaga gatcaaaggc gtgcagtgga tcccggactg gggccaggcg
     3181 cgtatcgagt cgatggttgc taaccgtcct gactggtgta tctcccgtca gcgcacctgg
     3241 ggcgtaccga tgtcactgtt cgtgcacaaa gacacggaag agctgcatcc gcgtactctc
     3301 gaactaatgg aagaagtggc aaaacgcgtt gaagttgacg gcatccaggc gtggtgggat
     3361 cttgatgcga aagagatcct cggcgatgaa gctgatcagt acgtgaaagt gccggacaca
     3421 ttggatgtat ggtttgactc cggatctacc cactcttctg ttgttgacgt gcgtccggaa
     3481 tttgccggtc acgcagcgga catgtatctg gaaggttctg accagcaccg tggttggttc
     3541 atgtcttccc taatgatctc caccgcgatg aaaggcaaag cgccgtatcg tcaggtactg
     3601 acccacggct ttaccgtgga tggtcagggt cgcaagatgt ctaaatccat cggcaatacc
     3661 gtttcgccgc aggatgtgat gaacaaactg ggcgcggata ttctgcgtct gtgggtggca
     3721 tcaaccgact acaccggtga aatggccgtt tctgacgaga tcctgaaacg tgctgccgat
     3781 agctatcgtc gtatccgtaa caccgcgcgc ttcctgctgg caaacctgaa cggttttgat
     3841 ccagcaaaag atatggtgaa accggaagag atggtggtac tggatcgctg ggccgtaggt
     3901 tgtgcgaaag cggcacagga agacatcctc aaggcgtacg aagcatacga tttccacgaa
     3961 gtggtacagc gtctgatgcg cttctgctcc gttgagatgg gttccttcta cctcgacatc
     4021 atcaaagacc gtcagtacac cgccaaagcg gacagtgtgg cgcgtcgtag ctgccagact
     4081 gcgctgtatc acatcgcaga agcgctggtg cgctggatgg caccaatcct ctccttcacc
     4141 gctgatgaag tgtggggcta cctgccgggc gaacgtgaaa aatacgtctt caccggtgag
     4201 tggtacgaag gcctgtttgg cctggcagac agtgaagcga tgaacgatgc gttctgggac
     4261 gagctgttga aagtgcgtgg cgaagtgaac aaagtcattg agcaagcgcg tgccgacaag
     4321 aaagtgggtg gctcgctgga agcggcagta accttgtatg cagaaccgga actgtcggcg
     4381 aaactgaccg cgctgggcga tgaattacga tttgtcctgt tgacctccgg cgctaccgtt
     4441 gcagactata acgacgcacc tgctgatgct cagcagagcg aagtactcaa agggctgaaa
     4501 gtcgcgttga gtaaagccga aggtgagaag tgcccacgct gctggcacta cacccaggat
     4561 gtcggcaagg tggcggaaca cgcagaaatc tgcggccgct gtgtcagcaa cgtcgccggt
     4621 gacggtgaaa aacgtaagtt tgcctgatga gtcaatcgat ctgttcaaca gggctacgct
     4681 ggctgtggct ggtggtagtc gtgctgatta tcgatctggg cagcaaatac ctgatcctcc
     4741 agaactttgc tctgggggat acggtcccgc tgttcccgtc gcttaatctg cattatgcgc
     4801 gtaactatgg cgcggcgttt agtttccttg ccgatagcgg cggctggcag cgttggttct
     4861 ttgccggtat tgcgattggt attagcgtga tcctggcagt gatgatgtat cgctcgaagg
     4921 ccacgcagaa gctaaacaat atcgcttacg cgctgattat tggcggcgcg ctgggcaacc
     4981 tgttcgaccg cctgtggcac ggcttcgttg tcgatatgat cgacttctac gtcggcgact
     5041 ggcacttcgc caccttcaac cttgccgata ctgccatctg tgtcggtgcg gcactgattg
     5101 tgctggaagg ttttttgcct tctagagcga aaaaacaata ataaaccctg ccggatgcga
     5161 tgctgacgca tcttatccgg cctacagatt gctgcgaaat cgtaggccgg ataaggcgtt
     5221 tacgccgcat ccggcaaaaa tcctcaaata taagagcaaa cctgcatgtc tgaatctgta
     5281 cagagcaata gcgccgtcct ggtgcacttc acgctaaaac tcgacgatgg caccaccgcc
     5341 gagtctaccc gcaacaacgg taaaccggcg ctgttccgcc tgggtgatgc ttctctttct
     5401 gaagggctgg agcaacacct gttggggctg aaagtgggcg ataaaaccac cttctcgttg
     5461 gagccagatg cggcgtttgg cgtgccgtca ccggacctga ttcagtactt ttcccgccgt
     5521 gaatttatgg atgcaggcga gccagaaatt ggcgcaatca tgctttttac cgcaatggat
     5581 ggcagtgaga tgcctggcgt gatccgcgaa attaacggcg actccattac cgttgatttc
     5641 aaccatccgc tggccgggca gaccgttcat tttgatattg aagtgctgga aatcgatccg
     5701 gcattggagg cgtaacatgc agatcctgtt ggccaacccg cgtggttttt gtgccggggt
     5761 agaccgcgct atcagcattg ttgaaaacgc gctggccatt tacggcgcac cgatatatgt
     5821 ccgtcacgaa gtggtacata accgctatgt ggtcgatagc ttgcgtgagc gtggggctat
     5881 ctttattgag cagattagcg aagtaccgga cggcgcgatc ctgattttct ccgcacacgg
     5941 tgtttctcag gcggtacgta acgaagcaaa aagccgcgat ttgacggtgt ttgatgccac
     6001 ctgtccgctg gtgaccaaag tgcatatgga agtcgcccgc gccagccgtc gtggcgaaga
     6061 gtcgattctc atcggccacg ccgggcatcc ggaagtggaa ggtacaatgg gccagtacag
     6121 taacccggaa gggggaatgt atctggtcga atcgccggac gatgtgtgga aactgacggt
     6181 caaaaacgaa gagaagctct cctttatgac ccagaccacg ctgtcggtgg atgacacgtc
     6241 tgatgtgatc gacgcgctgc gtaaacgctt cccgaaaatt gtcggtccgc gcaaagatga
     6301 catctgctac gccacgacta accgtcagga agcggtacgc gccctggcag aacaggcgga
     6361 agttgtgttg gtggtcggtt cgaaaaactc ctccaactcc aaccgtctgg cggagctggc
     6421 ccagcgtatg ggcaaacgcg cgtttttgat tgacgatgcg aaagatatcc aggaagagtg
     6481 ggtgaaagag gttaaatgcg tcggcgtgac tgcgggcgca tcggctccgg atattctggt
     6541 gcaaaatgtg gtggcacgtt tgcagcagct gggcggtggt gaagccattc cgctggaagg
     6601 ccgtgaagaa aacattgttt tcgaagtgcc gaaagagctg cgtgtcgata ttcgtgaagt
     6661 cgattaagtc attagcagcc taagttatgc gaaaatgccg gtcttgttac cggcattttt
     6721 tatggagaaa acatgcgttt acctatcttc ctcgatactg accccggcat tgacgatgcc
     6781 gtcgccattg ccgccgcgat ttttgccccc gaactcgacc tgcaactgat gaccaccgtc
     6841 gcgggtaatg tctcggttga gaaaaccacc cgcaacgccc tgcaactgct gcatttctgg
     6901 aatgcggaga ttccgctcgc ccagggagcc gctgtgccgc tggtacgcgc accgcgtgat
     6961 gcagcatctg tgcacggcga atcgggaatg gctggctacg actttgttga gcacaaccga
     7021 aagccaatcg ggataccagc gtttctggcg attcgtgatg ccctgatgcg tgcaccagag
     7081 cctgttaccc tggtggccat tggcccgtta accaatattg cgctgttact ttcacaatgc
     7141 ccggaatgta aaccgtatat tcgtcgtctg gtgatcatgg gcggttcttc tggtcgcggc
     7201 aactgtacac caaatgccga gtttaatatc gctgccgatc cagaagcggc tgcctgtgtc
     7261 ttccgtagcg gaatcgaaat cgtcatgtgc ggtctggatg tcaccaatca ggcaatatta
     7321 acccctgact atctcgctac tttgccggag ttgaatcgca ccggaaaaat gcttcatgca
     7381 ctgtttagcc actaccgtag cggcagtatg caaagcggtc tgcgaatgca cgatctctgc
     7441 gccatcgcct ggctggtgcg cccggactta ttcaccctca agccgtgttt tgtcgcggta
     7501 gaaactcaag gcgaatttac ctcaggcacg acggtggttg atatcgacgg ttgcctgggc
     7561 aaaccagcca atgtaaaggt ggcattggat ctggatgtga aaggcttcca gcagtgggtg
     7621 gctgaggtgc tggctctggc gtcgtaacct gtcacatgtt atcggcatgc agtcattcat
     7681 cgactcatgc ctttcactga tatccctccc tgtttatcat taatttctaa ttatcagcgt
     7741 ttttggctgg cggcgtagcg atgcgctggt tactctgaaa acggtctatg caaattaaca
     7801 aaagagaata gctatgcatg atgcaaacat ccgcgttgcc atcgcgggag ccggggggcg
     7861 tatgggccgc cagttgattc aggcggcgct ggcattagaa ggcgtgcagc tgggcgcggc
     7921 gctggagcgc gaaggatctt ctttactggg cagcgacgcc ggtgagctgg ctggtgcagg
     7981 gaaaacaggc gttaccgtgc aaagcagcct cgatgcggta aaagatgatt ttgatgtgtt
     8041 tatcgatttt acccgtccgg aaggtacgct gaaccatctc gctttttgtc gccagcatgg
     8101 caaagggatg gtgatcggca ctacggggtt tgacgaagcc ggtaaacagg cgattcgtga
     8161 cgccgccgcc gatattgcga ttgtctttgc cgccaatttt agcgttggcg ttaacgtcat
     8221 gcttaagctg ctggagaaag cagccaaagt gatgggtgac tacaccgata tcgaaattat
     8281 tgaagcacat catagacata aagttgatgc gccgtcaggc accgcactgg caatgggaga
     8341 ggcgatcgcc cacgcccttg ataaagatct gaaagattgc gcggtctaca gtcgtgaagg
     8401 ccacaccggt gaacgtgtgc ctggcaccat tggttttgcc accgtgcgtg caggtgacat
     8461 cgttggtgaa cataccgcga tgtttgccga tattggcgag cgtctggaga tcacccataa
     8521 ggcgtccagc cgtatgacat ttgctaacgg cgcggtaaga tcggctttgt ggttgagtgg
     8581 taaggaaaat ggtctttttg atatgcgaga tgtacttaat ctcaataatt tgtaaccaca
     8641 aaatatt
//
LOCUS       DAZPAC010000169         8634 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZPAC010000169 DAZPAC010000000
VERSION     DAZPAC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.169"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHUPM_003278"
     CDS             complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHUPM_003278"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR0870973.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHUPM_003279"
     CDS             complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHUPM_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR0870974.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="ACHUPM_003280"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="ACHUPM_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR0870975.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="ACHUPM_003281"
     CDS             complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="ACHUPM_003281"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR0870976.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="ACHUPM_003282"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="ACHUPM_003282"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR0870977.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7826..8137)
                     /gene="ymdA"
                     /locus_tag="ACHUPM_003283"
     CDS             complement(7826..8137)
                     /gene="ymdA"
                     /locus_tag="ACHUPM_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR0870978.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="ACHUPM_003284"
     CDS             complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="ACHUPM_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR0870979.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2156 a         2248 c         2051 g         2179 t
ORIGIN      
        1 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcgacgtg aacgctttac
       61 ctaacccaca aaagcttgca aaatcaataa attgcatgaa cgatgtaggc ctgataagcg
      121 tagcgcatca ggcaactaca tttttattgc gaagccgcat ccggtttacg caatgccagt
      181 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc
      241 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga
      301 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
      361 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
      421 aatgacgggt taaacactgc gtgcataaag ccatcgtcaa gggagcgttg gcgattcatc
      481 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
      541 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
      601 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
      661 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
      721 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
      781 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
      841 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
      901 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
      961 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
     1021 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     1081 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     1141 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     1201 atcaggttac cgtggcacca tcggcggtca cgttttagct catcaagcaa gttaggcggc
     1261 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     1321 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
     1381 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
     1441 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa cagtggccca
     1501 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
     1561 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac cagcccgcac
     1621 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     1681 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
     1741 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     1801 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
     1861 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
     1921 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     1981 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     2041 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
     2101 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
     2161 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
     2221 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     2281 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
     2341 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     2401 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
     2461 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     2521 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
     2581 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     2641 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     2701 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     2761 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
     2821 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     2881 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
     2941 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     3001 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     3061 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     3121 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
     3181 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     3241 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     3301 ttccatcgag aagctgctaa ccgctaaatg tttcgggtta ttcaacggac gccagatcca
     3361 ttcgccatta ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt
     3421 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     3481 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     3541 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     3601 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     3661 aaattcttcc cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
     3721 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     3781 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
     3841 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     3901 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     3961 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     4021 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     4081 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     4141 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     4201 acgcattttc atcatttgta gtttatgttt cataattagt aaagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa cagaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca attttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
     4801 cgttatatcc ggctactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatcccct tactgacatt
     4921 gccgcaattt attatgctgc aatacgtcaa agggaaagcg gaaagttggc ctgggctgtc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgcgt atggatattt aaacgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccgcccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt tgccgttctt
     5281 tatcctcggt gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt acccttgcag cagcattggc atttgtcgct tatttactca accagcgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggtct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg
     5521 ggtgacttac tttgttaacg catcgctatt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttttctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taatctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcatac
     5941 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa agttttagca tgcaggctgg ctccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact actttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc ggacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgttt tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaacgga ctggattcca tttggcgtaa gtaacgatgc
     6541 gtcatggcat cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa cgtcctctac cacgggtcct attgccataa catctaaatc cgaaaaaagt
     6721 ggctcttccc ctgccccaaa ataggcatca ccaatatttc gtcctcccac cagggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atacggcgat tgagacggga aaagtcggtg
     6841 atataaccaa gcggacgcag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cggggatggc tgtcaagcaa gcgtaaaata tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac
     7021 cgacccgaca tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac
     7321 tgcgatttca gccgctgccg cacgagggta accataaacc ccagtactga ttgcaggaaa
     7381 agccactgac gtatagctgt ttgccgccac cagtcgcagg ctattcagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
     7801 tcagcaacgt gcgttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
     7861 tctgtttatc aagatactgc acttttactg aggctatttg cgcgttttta acattaccgc
     7921 tcattaacgc tttgctggaa taggttttgc ccggaacaga accattctgt gggcagttca
     7981 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
     8041 aatggatcac acccccttgc gcggtgcttc ctgcgatggc aataaaagga aaaaacaaac
     8101 tgccgagcat aagagagttt aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
     8281 gccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aataggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
     8461 atatttgtac tctgcacaga caagattgag taagagtgac ttcaggaata atggtataca
     8521 catccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
     8581 acgcattcat attcttctcc cgaaaaaaac agggcttgcg ccctgtttct gtaa
//
LOCUS       DAZPAC010000170         8600 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZPAC010000170 DAZPAC010000000
VERSION     DAZPAC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8600
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.170"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(178..1842)
                     /gene="glnS"
                     /locus_tag="ACHUPM_003285"
     CDS             complement(178..1842)
                     /gene="glnS"
                     /locus_tag="ACHUPM_003285"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR0870980.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2102..4048)
                     /gene="nagE"
                     /locus_tag="ACHUPM_003286"
     CDS             complement(2102..4048)
                     /gene="nagE"
                     /locus_tag="ACHUPM_003286"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR0870981.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            4381..5181
                     /gene="nagB"
                     /locus_tag="ACHUPM_003287"
     CDS             4381..5181
                     /gene="nagB"
                     /locus_tag="ACHUPM_003287"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR0870982.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5241..6389
                     /gene="nagA"
                     /locus_tag="ACHUPM_003288"
     CDS             5241..6389
                     /gene="nagA"
                     /locus_tag="ACHUPM_003288"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR0870983.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            6398..7618
                     /gene="nagC"
                     /locus_tag="ACHUPM_003289"
                     /gene_synonym="nagR"
     CDS             6398..7618
                     /gene="nagC"
                     /locus_tag="ACHUPM_003289"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR0870984.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7666..8418
                     /gene="nagD"
                     /locus_tag="ACHUPM_003290"
     CDS             7666..8418
                     /gene="nagD"
                     /locus_tag="ACHUPM_003290"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFR0870985.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(8469..8600)
                     /locus_tag="ACHUPM_003291"
                     /pseudo
     CDS             complement(8469..8600)
                     /locus_tag="ACHUPM_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2103 a         2270 c         2192 g         2035 t
ORIGIN      
        1 gccggatgcg gcgtgaacgc cttaaccggc ctacaaaagc acgcaaactc aatatattgc
       61 agagatcatg taggcctgat aagcgtagtg catcaggcaa tttagcgttt gaaactgttt
      121 gtctgttcct tgccggatgc gacgtaaacg ccccatccgg catagcgaaa cttaaaatta
      181 ctcgcctact ttcgcccagg tatcacgcag cccaacggtg cggttaaata ccggtttttc
      241 cgccgtagaa tggcggctat cgaggcagaa gtaaccttca cgctcaaact ggaatgcttt
      301 acccgcaacc gcatctttca gcgacggttc agcaaagccc tgtttgatca ccagcgattc
      361 cgggttaatc accgacagga aatcatccgc agcaccaggg ttcggcacgc tgaacagacg
      421 gtcgtacaga cggatttcaa ccggcagcgc atgtgccgcg ctcacccagt gaataacacc
      481 tttgacttta cgaccatctg ccggatcttt gcttaaggtg tcggcgtcat aagtacagaa
      541 gatggtggtg atattgcctt cggcatcttt ctccacgcgt tctgccttaa tgacgtaagc
      601 attacgcaga cgcacttctt tacccagcac cagacgtttg tactgcttgt tagcttcttc
      661 gcggaaatcg gcgcgatcga tccaaatctc accgctaaac ggcacctggc ggctacccat
      721 ttccggtttg ttcggatggt tcggcatggt aaccatttcg ccttcgccct gatagttttc
      781 gataaccagt ttcaccggat cgataaccgc cattgcgcgc ggggcgtttt cgttgagatc
      841 ttcacggatg caggattcca gcgacgccat ctcaatggtg ttgtcctgct tggtcacgcc
      901 gatgcgtttg cagaactcac gaatagaagc agcagtgtaa ccacgacgac gcagaccgga
      961 aatggtcggc atacgcgggt catcccagcc ttcaacgtgc ttgtcggtca ccagcaggtt
     1021 cagcttacgc ttggacatca cagtgtattc cagattcagg cgcgagaatt cgtactggcg
     1081 cgggtgaaca ggaatggtga tgttgtccag cacccagtcg tacaggcgac ggttgtcctg
     1141 gaactcaagc gtacacagag agtgcgtaat accttccagg gcatcgctga tgcagtgggt
     1201 gaagtcgtac atcgggtaga tgcaccactt gttgccagtc tggtggtgtt cagcaaattt
     1261 aatgcggtac agcaccggat cgcgcatcac gataaacggt gaagccatgt cgattttcgc
     1321 acgcaggcag gctttacctt cttcaaaacc accggcacgc attttttcga acagcgccag
     1381 gttctcttca acgctgcggt cgcggtacgg gctgttttta cccggttgcg tcagggtgcc
     1441 gcggtattcg cggatctgtt ccggcgtcag ttcatcaacg tacgccaggc ctttattgat
     1501 cagttcgatc gcataggcgt ggagctgatc aaaataatcg gaggagtaac ggacgttacc
     1561 agaccagtga aaacctaacc actctacgtc gtttttgatc gactcaacat actcgatatc
     1621 ttcttttacc gggttagtgt cgtcgaaacg caggttgcac tggcctttat agtcctgggc
     1681 gatcccgaag ttcaggcaga tagatttcgc atggccaata tgcagatagc cattcggctc
     1741 cggcgggaaa cgggtgtgta ctgtggtgtg cttaccactg gccagatctt catcgatgat
     1801 ctgacggata aagttagtcg ggcgggcttc tgcctcactc atcgtggatt cctcaaagcg
     1861 taaacaacgt ataacggcgt atgatcttat aagcgggaca ggctgacaac tgttagttag
     1921 cgaaaatcga aaatctcact gagattatgg ggatgattga tgaaaaaaaa aagcggcaga
     1981 gaagatctcc accgcttttg ccagtcccgc gtcattgttg gatgcgcgtc ttatccaacc
     2041 aggactgagc ttttgtaggc ctgataagac gcgtcaagcg tcgcatcagg cataaagcag
     2101 attacttttt gatttcatac agcggtgttt gacccgccac aacattgccc tgagctttaa
     2161 tgatcaagcc actgaaatcg tcgatattgc tgcaaaccac cgggctaatc atcgaacggg
     2221 cgttagcgtt caggtaatcc agatccattt ccagaatcgg ttgccctgcg ctcacctgcg
     2281 cgccctcttc caccagacgt ttaaagcctt taccttccag cgctacggtg tcgataccca
     2341 tgtggacgac aatctccgcg cctttttcgg tttctaggca gaacgcgtgg ttggtgttga
     2401 agattttcac gattgtccct gcggctggtg atacgacgat tttatctgtc ggtttcaccg
     2461 ccacaccgtc acccaccgct ttgctggcga atgcttcgtc agaaacctga tccagtgcca
     2521 cgacatcacc ggtaatcggc gataccagct ccgcgataga taccgcgttt ggtacagcct
     2581 gcggttttgc tacaggagcg gcagttgccg gagttgcttc agctgacgca gctgctaccg
     2641 ggccacgggc aacgactttc ttcatcgcat cgccaatgga ttctgctttc gcgccaacaa
     2701 tcacctgaat ggtctgtttg ttcagtttta ctacaccaga agcacccaga cgtttacaca
     2761 tcgcatcgtt gacgcgagct gagtcagcca cggtaaggcg cagacgggta atacaggcgt
     2821 caatcgcttt caggttgtca gtaccgccaa ccgcagcaat atagttggtt gccagttgag
     2881 tcagaccttc ttcagtattg ctgttagcct cttcagttac gatctcgtct tctttatctt
     2941 cacgacccgg cgttttcagg ttgaacatgc ggataaccaa actgaacacc acgaagtaga
     3001 tagcgaagaa gacaacaccc atcaccagca gcatccagac gttctggctg gcggccggca
     3061 ggttatacat caacgcgtag tcgatagccc ccgcagagaa agagaagcca gcatggatac
     3121 ccagcagcgt tgccacaaac aggctgatac cggtcagcag tgcgtgcagg aggtacagca
     3181 gcggagcaag gaacatgaac aggaattcca gcggctcagt cacaccggtc aggaacgcag
     3241 taacagcaac agaaagcagc atcccgccaa ccatcggacg acgctctttc ggtgctgcga
     3301 agtacatcgc cagcgccgca cccggcagac cgaacatcat gatcgggaag aagccggaca
     3361 tgaacatccc cgcggtgccg tcaccagcgt agaaacggtt gatgtcgccg tggaaaaccg
     3421 tacccgccgc gttggtgaat tcaccaatct ggaaccaggc gatggtgttc agcacctgat
     3481 gcagaccggt tgggatcagc agacggttga tgaaaccaaa gataccggaa cccagcgcgc
     3541 ctgcagaaac gatccactcg ccgcctgcat ggatagcgtg ctgtactggc ggccagacgt
     3601 aaccaaaaat ggccgccagc accaggcaga aaaagccggt ggcaatcggc acaaagcgtt
     3661 tgccgccgaa gaagctcagg aagtccggca gtttaatatc ggaccaacgg ttataggctg
     3721 cgccaccaac cagaccggta atgatacccg ccagtacacc catgttaatt tctgggttga
     3781 tggtcaccat cgctttggtt aacacaaagt aacctaccgc acccgccagc gccgccgcac
     3841 ctgcgctgtc tttcgaccag ctggatgcca caccgatggc gaagattaat gcgaggttat
     3901 caaaaatcgc accgcccgcc tgggcaataa acgcaacgtt aagtaaatct ggctgaccga
     3961 atcgcagcaa cagtgccgcc accggcagca ccgcgatagg gagctgtaac gccctaccga
     4021 gtcgctggaa aaaacctaaa atattcatct tattccccct acgagaaccc tatttggctc
     4081 gtttaaagcc gtatttttat tttgctgcaa attgtactgc cgatgttctg taatcagatt
     4141 gttagatcat ctgctacaga gtgtgtgaaa atttaattcg tatcgcaaat taaacgcgtg
     4201 ccttttgtga gttttgtcac caaatatcgt tatcatcact cccttttact ggctaaagca
     4261 gaaaacttat tttatcattc aaaaaatcag gtcggattga cgcctgtctg cgcaaatcca
     4321 ggttacgctt aaagatgcct aatccgccaa cggcttacat tttacttatt gaggtgaata
     4381 atgagactga tccccctaac taccgctgaa caggtcggca aatgggctgc tcgccatatc
     4441 gtcaatcgta tcaatgcgtt caaaccgact gccgatcgtc cgtttgttct gggcctgccg
     4501 actggcggca cgccgatgac cacctataaa gcgttagtcg aaatgcataa agcaggccag
     4561 gtcagcttta agcacgttgt caccttcaac atggacgaat atgtcggtct gccgaaagag
     4621 catccggaaa gctactacag ctttatgcac cgtaatttct tcgatcacgt tgatattcca
     4681 gcagaaaaca tcaaccttct caacggcaac gccccggata tcgacgccga atgccgccag
     4741 tatgaagaaa aaatccgttc ttacggaaaa attcatctgt ttatgggcgg tgtaggtaac
     4801 gacggtcata ttgcatttaa cgaaccggcg tcttctctgg cttctcgtac tcgtatcaaa
     4861 accctgactc atgacactcg cgtcgcgaac tctcgtttct ttgataacga tgtcaatcag
     4921 gtgccaaaat atgccctgac tgtcggtgtt ggtacattgc tggatgccga agaagtgatg
     4981 attctggtgc tgggtagcca gaaagcactg gcactgcagg ctgcagttga aggttgcgtg
     5041 aaccacatgt ggaccatcag ctgtctgcaa ctgcatccaa aagcgatcat ggtgtgcgat
     5101 gaaccttcca ccatggagct gaaagttaag actttaagat atttcaatga attagaagca
     5161 gaaaatatca aaggtctgta attgttatcc ctgccctggc tccttgctca gggcaatatt
     5221 ttttaaaatc ggggggcaga atgtatgcat taacccaggg ccggatcttt accggccacg
     5281 aatttcttga tgaccacgcg gttgttatcg ctgatggcct gattaaaagc gtctgtccgg
     5341 tagcggaact gccgccagag atcgaacaac gttcactgaa cggggccatt ctctcccccg
     5401 gttttatcga tgtgcagtta aacggctgcg gcggcgtaca gtttaacgac accgctgaag
     5461 cggtcagcgt ggaaacgctg gaaatcatgc agaaagccaa tgagaaatca ggctgtacta
     5521 actatctgcc gacgcttatc accaccagcg atgagctgat gaaacagggc gtgcgcgtta
     5581 tgcgcgagta cctggcaaga catccgaatc aggcgttagg tctgcatctg gaaggtccgt
     5641 ggctgaatct ggtaaaaaaa ggcacccata atccgaattt tgtgcgtaag cctgatgccg
     5701 cgctggtcga tttcctgtgt gaaaacgccg acgtcattac caaagtgacc ctggcaccgg
     5761 aaatggttcc tgcggaagtc atcagcaaac tggcaaatgc cgggattgtg gtttctgccg
     5821 gtcactccaa cgcgacgttg aaagaagcga aagccggttt ccgcgcgggg attacctttg
     5881 ccacccatct gtacaacgcg atgccgtata ttaccggtcg tgaaccgggc ctggcgggcg
     5941 cgatcctcga cgaagctgac atttattgcg gtattatcgc tgatggcctg catgttgatt
     6001 acgccaacat tcgtaacgct aaacgcctta aaggcgacaa actgtgtctg gttaccgacg
     6061 ccaccgcgcc agcaggtgcc aacattgaac agttcatttt tgcgggtaaa acaatatact
     6121 accgtaacgg actttgtgtg gatgagaacg gtacgttaag cggttcatcc ttaaccatga
     6181 ttgaaggcgt gcgtaatctg gtcgaacatt gcggtatcgc actggatgaa gtgctgcgta
     6241 tggcgacgct ctatccggcg cgtgcgattg gcgttgagaa acgtctcggc acactcgccg
     6301 caggtaaagt agccaacctg acctcattca cacctgattt taaaatcacc aagaccatcg
     6361 ttaacggtaa cgaggtcgta actcaataag agaaagtatg acaccaggcg gacaagctca
     6421 gataggtaat gttgatctcg taaaacagct taacagcgcg gcggtttatc gcctgattga
     6481 ccagtacggg ccaatctcgc ggattcagat tgccgagcaa agccagcttg cccccgccag
     6541 cgtaaccaaa attacgcgtc agcttatcga acgcgggctg atcaaagaag ttgatcagca
     6601 ggcctccacc gggggccgcc gcgctatctc catcgtcacc gaaacccgca atttccacgc
     6661 aatcggcgta cggcttggtc gtcacgacgc caccatcacc ctgtttgatc tcagcagcaa
     6721 agtgctggca gaagaacatt acccgctgcc ggaacgtacc cagcaaacgc tggaacatgc
     6781 cctgttgaat gccattgctc agtttattga tagctaccag cgcaaactgc gcgagctgat
     6841 cgcgatttcg gtgatcctgc cagggcttgt tgacccggac agcggcaaaa ttcattacat
     6901 gccgcatatt caggtagaaa actgggggct ggtagaagct ctggaagaac gttttaaagt
     6961 gacctgtttc gttggtcacg atatccgtag tctggcgctg gcggagcact acttcggtgc
     7021 aagtcaggat tgcgaagact ccattctggt gcgtgtccat cgcggaaccg gggccgggat
     7081 tatctctaac gggcgcattt ttattggccg caacggtaac gtcggtgaaa ttggccatat
     7141 tcaggtcgaa ccgctgggtg aacgctgcca ctgcggcaac tttggctgcc tggaaactat
     7201 cgccgccaac gctgccattg aacaacgggt gttgaatctg ttaaagcagg gctaccagag
     7261 ccgcgtgccg ctggacgact gcaccatcaa aactatctgc aaagccgcga acaaaggcga
     7321 tagtctggcg tcggaagtaa ttgagtatgt cggtcgtcat ctgggtaaaa ccatcgccat
     7381 tgctatcaac ttatttaatc cgcaaaaaat tgttattgcc ggtgaaatca ccgaagccga
     7441 taaagtgctg ctccctgcta ttgaaagctg cattaatacc caggcgctga aggcgtttcg
     7501 tactaatctg ccggtggtac gttctgagct ggatcaccgc tcggcaatcg gcgcttttgc
     7561 gctggtaaaa cgcgccatgc tcaacggtat tttgctccag catttgctgg aaaattaatg
     7621 tgcttttata gtggcgctta ttgttgtcaa tattctgggt agtccatgac cattaaaaat
     7681 gtaatttgcg atatcgacgg cgtgctgatg cacgataacg tcgccgtacc gggtgcagcg
     7741 gaatttttgc acgggattat ggataaaggc ctgccgctgg tgttgctgac caactatcct
     7801 tcgcagaccg ggcaagatct ggcgaaccgc tttgccactg ccggtgtcga tgtgccggat
     7861 agcgtgtttt atacctctgc gatggcgacc gccgatttcc tgcgtcgcca ggaaggcaag
     7921 aaagcatatg tggtgggcga aggcgcactg attcatgaac tgtacaaagc cggtttcact
     7981 attaccgatg tgaaccctga tttcgtgatt gttggcgaaa cgcgttccta caactgggac
     8041 atgatgcata aagcggccta tttcgtcgct aacggtgcac gctttatcgc caccaacccg
     8101 gacacccacg ggcgcggttt ttatcccgct tgtggcgcgt tgtgtgcagg gattgagaaa
     8161 atctccgggc gcaaaccgtt ctatgttggt aagcccagcc cgtggatcat ccgcgcagca
     8221 ttaaacaaaa tgcaggcgca ttcggaagaa acggtgattg tcggcgataa cttgcgcacc
     8281 gacattctgg cgggcttcca ggcaggtctg gaaacgattc tggtgctttc tggtgtttca
     8341 tcgctcgacg atatcgacag tatgccgttc cgcccaagct ggatttaccc gtcagtcgct
     8401 gaaatcgacg ttatctgaat accacccacg tctgcggacg tggttggctc tgatgacaaa
     8461 cgccaaactg cctgatgcgc tacgcttatc aggcctacgc agctcctgca atatattgaa
     8521 ttttcatgct tttgtaggcc ggataaggcg ttcacgccgc atccggcatg aacaaatcgc
     8581 actttatcag caatttggcc
//
LOCUS       DAZPAC010000171         8533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZPAC010000171 DAZPAC010000000
VERSION     DAZPAC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.171"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="ACHUPM_003292"
     CDS             complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="ACHUPM_003292"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR0870986.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHS"
     gene            complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="ACHUPM_003293"
     CDS             complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="ACHUPM_003293"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFR0870987.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(1322..2986)
                     /locus_tag="ACHUPM_003294"
     CDS             complement(1322..2986)
                     /locus_tag="ACHUPM_003294"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFR0870988.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            3028..3861
                     /locus_tag="ACHUPM_003295"
                     /pseudo
     CDS             3028..3861
                     /locus_tag="ACHUPM_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            3938..5020
                     /gene="lacI"
                     /locus_tag="ACHUPM_003296"
     CDS             3938..5020
                     /gene="lacI"
                     /locus_tag="ACHUPM_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFR0870989.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            5143..8217
                     /gene="lacZ"
                     /locus_tag="ACHUPM_003297"
     CDS             5143..8217
                     /gene="lacZ"
                     /locus_tag="ACHUPM_003297"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFR0870990.1"
                     /translation="MIMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPHWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            8269..>8533
                     /locus_tag="ACHUPM_003298"
     CDS             8269..>8533
                     /locus_tag="ACHUPM_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0870991.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         1941 a         2390 c         2333 g         1869 t
ORIGIN      
        1 aactatggcc gcccatcgag ttgcccagca ggtggatttt ggcgatatcc agttgatcca
       61 ccacgctttt caggattcgt gcattaagat ccgatcgtga accactatta acgatcgaat
      121 cgcttttgcc ccaacccgga caatccagca ggatcacccg atagcccgcc tctaccagcg
      181 gatcgatatt gcggctgaag ttcgcccagc cagtagcacc cgggccggaa ccatgcagca
      241 ggacaacggt ttcgtcgcct tgtccgcagt cattaaaatg gatgcgcagc gttttacccg
      301 cttcttctac attcagaaaa cggctggtgg cggcttcggt ttgtggctga taactcatca
      361 tcttctcctg catattcagt tctctgttct ggcgcttaac gagccaaatc cggcaatcca
      421 ctccgggatt gggcggtaat aacgcccttc gctacgccag ttgccaaatg cagaaatagc
      481 ggcaaaagcg gcgacccagg ttttgatttc atgtgtcgac tttccggcaa tggcggaaag
      541 ctcttcgtta ctgacggcat ccagttcctg tatgcgtccc tgctccagca aagtcatgaa
      601 ctggttatcc caaatcgggt tgagcggatg cagagttctc tgatcctcaa caaacttctc
      661 agcggcgcta atcacccttt gctgacgcaa ttcgcgctca ctggcgggta aatctttccc
      721 gctccccaac agacggtcgc gcatatgggc atcggctttc gccagttcgg gcaccggcgg
      781 ctgatgggaa agcccaccgg aacccaggaa cagcacgcgt ttattgagag tgctggtgaa
      841 acgtccaatg gcttcaccca acatgcgggt acgctggaaa ccgggcagcg gcgtggcgac
      901 accgttgatg aacacaggca gaactggcac cttatccagc ccaccgagca ggaactccag
      961 cggctgggcg aacccgtggt ccacctgcat acagtaagaa acggcaagat cgatcccgct
     1021 cttcatgaca gcatgcgcac aggcctccgc cagctccaca ggcacgggca gctctcctgc
     1081 cgcactgccg aaatcaccaa ttgccgtcgc tccaacgcct aaacagaacg gtggcatcac
     1141 gtcatagaaa aagccgttgt agtgatctgg cgcaaacagc accaccagtt caggggagaa
     1201 agccgcaata cgctcgcggg cgctggcaat cacgccattg acctcatcga gtacctcttg
     1261 cgccgggtcg acatacccca ccaacggcga gtgggaaaga cagtgaagat aagcgtgcat
     1321 atcaggctac cttttcgaca gaaacgtcgg catcagggcg ggtcagcgtc atcaccgacg
     1381 ccagtttatt cagcgtattg cccagggttt gcggaatggc ggtggcggca acaaagcgat
     1441 ccgggcgcat caccaccagc gaagcattgt gttgcgcgaa ccagctacgc agacgacctt
     1501 gcgtatcgcc cacgcgtagc acgccgtcgt ggttatcctg tgcggtatga atttgcactt
     1561 ccggcaccac ctgaatgaag cgtgtgccca acgcgcgcca ctgctggatt tgctcgtcgc
     1621 tcatccccca cagtggattg catccccagc caattaccgc gaagttcgcg ccgatcgcgt
     1681 tatcgagcag cgtcacgtcg ccgttttcca gcgtgacttt cggctgaata aacatcttgc
     1741 cgaccggaga gtgcttcgct tcgccctcac gcaccagcgc accgccgtaa tattgcggca
     1801 tcggcttgaa gcgcatttcg aggaagtagc gttttactgg cggcagataa ttcaacagcc
     1861 aggaaacgcc gtcacgtaac gtaccctgcc agcgtttcgg cggagccagc acgttgcccg
     1921 ccgtcacgga caggtcaatc atcgctttgg cgtgatcgcg acgttcttgt tgataggtat
     1981 cgagcagcgc atcgcgggct ttcccctgga taaccaacgc cagtttccag gcgaggttaa
     2041 aggcgtcgcg cataccactg ttatagccct gcccctgcca caccggcatg atgtgcgcgg
     2101 catcgcccgc cagcagtacg cgatcaatac ggaaacgttg cgccagtcgc gcgttatggg
     2161 tgtagacacg ctggcgaatc aattcaacat tgtccggatt aggcagcact ttgcttaaca
     2221 gcttgcgcat attttgcggc tcacgcagct gctcttcggt ttctcccggc atcaccataa
     2281 attcaaagcg acgtaccgca tgaggcagcg cggcagaaac atacgggcgc accggatcgc
     2341 aacacaaata gatatgcggc gtacttaacg gatcgttggc gatatctacc acaatccact
     2401 gatttggcgc agttttacct tcaaacggca cattcagggt gcgacgaaca aaacttgccc
     2461 caccatcaca ggccaccagc cactgggctt tgatcgtttc ccgctgccct tctgccgttt
     2521 tcaggtgcaa ggtcacttcg tcatcttgct ggctgaaggc ctccagctcg cgggaaaaca
     2581 agcagcgcac attcggaaaa cgcgacaacc cttccagcat caccgcatcg acctgtggct
     2641 gaataaaggc gttacggcgc ggccagccaa attcatcggt cattggctga atatcagcaa
     2701 aacagcggcc ttttggggtg agaaaacgca tcgcgtgcca cggtgtagtg tgcggcagaa
     2761 cattctcgac caggccgacc gactgcatgg tgcgcagcgc ctcgtcatca ataccaatcg
     2821 cacgcgggta gtcgatcaac ttatcgagtt tctccaccac cagcacgtca atgcccatct
     2881 gaccgagata gttcgccatc atcagcccaa ccggaccggc accagcgatc gccacctgaa
     2941 cgctatggtt aacagcaggc tggatgtcag ggtgttgtat tgccatttct gcacaacgtt
     3001 gttttaacta tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc
     3061 gtgcgcggct taacccgcgg tctaatgtta ttaaatatgt taaataaact tgatggcggt
     3121 gccagcgtcg ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg
     3181 ctggagacgc tgcaggaaga gggatatgtc cgccgtagcc cctccgatga cagttttcgt
     3241 ctgaccatca aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca
     3301 ctggcggccc cgctgctggg cgatctgttg cgcgaagtgg tatgaccaac agatgtgtcc
     3361 accctggatg ttgatgcaat ggtggtacgc gaaaccaccc accgtttcag ccgcttatcc
     3421 tttcaccggg caatggtcgg gcgacgttta ccgctcctga aaaccgcctc gggcctgacc
     3481 tggctggcct tttgcccgga acaagaacgc aaggaattaa tcgaaatgtt agccgcccgc
     3541 cccggtgatg actatcaact ggcacgagaa ccgttaaagc tgcaagccat tctggcgcgc
     3601 gcgcgcaaag agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc
     3661 tctatcgccg taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat
     3721 atggcgagcg caatgaccat tgaacaggca gcggaaaagc atcttccggc gctacaacgg
     3781 gtagcaaaac agatcgaaga aggggttgaa tcgcaggcta ttctggtggc cggacggcga
     3841 agcggcgtgc atttacgttg acaccatcga atggcgcaaa acctttcgtg gtatggcatg
     3901 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgctatacga
     3961 tgtcgcagag tatgccggtg tctcttatca gaccgtttct cgcgtggtga accaggccag
     4021 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     4081 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     4141 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     4201 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
     4261 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
     4321 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
     4381 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
     4441 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
     4501 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
     4561 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
     4621 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
     4681 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
     4741 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac
     4801 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
     4861 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     4921 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     5041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     5101 gtgaaattgt tagcggataa caatttcaca caggaaacag ctatgatcat gattacggat
     5161 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5221 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5281 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
     5341 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc
     5401 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat
     5461 cccattacgg tcaatccgcc ttttgttccc gcggagaatc cgacgggttg ttactcgctc
     5521 acatttaata ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggt
     5581 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagc
     5641 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggaaaaaa ccgcctcgcg
     5701 gtgatggtgc tgcgctggag tgacggtagt tatctggaag atcaggatat gtggcgaatg
     5761 agcggcattt tccgtgacgt ctcgttgctg cacaaaccga gcacacaaat cagcgatttc
     5821 catgttgcca ctcactttaa tgatgatttc agccgcgcgg tactggaggc agaagttcag
     5881 atgtacggcg agctgcgcga tgagctgcgg gtgacggttt ctttgtggca gggtgaaacg
     5941 caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat
     6001 gccgatcgcg tcacgctacg tctgaacgtc gaaaacccgg cgctgtggag cgccgaaatc
     6061 ccgaatctct atcgtgcggt ggttgaactg cacaccgacg acggcacgct gattgaagca
     6121 gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac
     6181 ggcaagccgt tgctgattcg cggcgttaac cgtcacgagc atcatcctct gcatggtcag
     6241 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac
     6301 gccgtgcgtt gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgcttc
     6361 ggcttgtacg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
     6421 ctgaccgatg atccgcactg gttacccgcg atgagcgaac gcgtaacgcg aatggtgcag
     6481 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgagtc aggccacggc
     6541 gctaatcacg acgcactcta tcgctggatc aaatctgtcg atccatcccg cccggtgcag
     6601 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc
     6661 gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg
     6721 ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt
     6781 cttggcggct tcgctaaata ctggcaggcg tttcgtcagt acccccgttt acagggcggc
     6841 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaatgg caatccgtgg
     6901 tcggcttacg gcggtgattt tggcgataca ccgaacgatc gccagttctg catgaacggt
     6961 ctggtctttg ccgaccgcac gccgcatcca gcgctgacag aagcaaaaca ccagcagcag
     7021 tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt
     7081 catagcgata acgagcttct gcactggtcg gtggcgttgg atggcaagcc gctggcaagc
     7141 ggtgaaatgc ctctggatgt tgctccacaa gataaacagt tgattgaact gcctgaacta
     7201 ccgcagccgg agagcgccgg acaactctgg cttacggtac acgtagtgca accgaacgcg
     7261 accgcatggt cagaagccgg acacatcagc gcctggcagc agtggcgtct ggcggaaaac
     7321 ctcagcgtga cactccccgc cgcgccccac gccatcccgc aactgaccac cagcgaaacg
     7381 gatttttgca tcgagctggg taataaacgt tggcaattta accgccagtc aggctttctt
     7441 tcacagatgt ggattggcgc tgaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc
     7501 cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga cccgaacgcc
     7561 tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc
     7621 acggcagata cacttgccga cgcggtgctg attacgaccg ctcacgcgtg gcagcatcag
     7681 ggaaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg
     7741 attaccgttg atgttgaagt agcgagcgat acaccgcatc cggctcggat tggcctgacc
     7801 tgccagctgg cgcaggtagc agaacgggta aactggctcg gattagggcc gcaagaaaac
     7861 tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg
     7921 tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat
     7981 tatggcccac accagtggcg cggcgacttc cagttcaata tcagtcgcta cagccaacaa
     8041 caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat
     8101 atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtttcagcg
     8161 gaattccagc ttagcgccgg tagctaccat taccagttgg tctggtgtca aaaataataa
     8221 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta
     8281 aaaaacacaa acttttggat gttcggttta ttctttttct tttacttttt tatcatgggg
     8341 gcctacttcc cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat
     8401 acgggtatta tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt
     8461 ctgctttctg acaaactcgg gctgcgcaaa tacctgctgt ggattattac cggcatgtta
     8521 gtgatgtttg cgc
//
LOCUS       DAZPAC010000172         8486 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZPAC010000172 DAZPAC010000000
VERSION     DAZPAC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.172"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..2519)
                     /gene="traC"
                     /locus_tag="ACHUPM_003299"
     CDS             complement(<1..2519)
                     /gene="traC"
                     /locus_tag="ACHUPM_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFR0870992.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREG"
     gene            complement(2645..2992)
                     /locus_tag="ACHUPM_003300"
     CDS             complement(2645..2992)
                     /locus_tag="ACHUPM_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870993.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(3020..3238)
                     /locus_tag="ACHUPM_003301"
     CDS             complement(3020..3238)
                     /locus_tag="ACHUPM_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870994.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPFNSVMETLNGGGQ"
     gene            complement(3337..3810)
                     /locus_tag="ACHUPM_003302"
     CDS             complement(3337..3810)
                     /locus_tag="ACHUPM_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870995.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKVC"
     gene            complement(3803..4216)
                     /locus_tag="ACHUPM_003303"
     CDS             complement(3803..4216)
                     /locus_tag="ACHUPM_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0870996.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(4209..4430)
                     /gene="traR"
                     /locus_tag="ACHUPM_003304"
     CDS             complement(4209..4430)
                     /gene="traR"
                     /locus_tag="ACHUPM_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFR0870997.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(4565..5080)
                     /gene="traV"
                     /locus_tag="ACHUPM_003305"
     CDS             complement(4565..5080)
                     /gene="traV"
                     /locus_tag="ACHUPM_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFR0870998.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(5077..5397)
                     /locus_tag="ACHUPM_003306"
     CDS             complement(5077..5397)
                     /locus_tag="ACHUPM_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFR0870999.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(5384..5974)
                     /gene="traP"
                     /locus_tag="ACHUPM_003307"
     CDS             complement(5384..5974)
                     /gene="traP"
                     /locus_tag="ACHUPM_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFR0871000.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(5964..7391)
                     /gene="traB"
                     /locus_tag="ACHUPM_003308"
     CDS             complement(5964..7391)
                     /gene="traB"
                     /locus_tag="ACHUPM_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFR0871001.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGTSKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(7391..8119)
                     /gene="traK"
                     /locus_tag="ACHUPM_003309"
     CDS             complement(7391..8119)
                     /gene="traK"
                     /locus_tag="ACHUPM_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFR0871002.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(8106..>8486)
                     /gene="traE"
                     /locus_tag="ACHUPM_003310"
     CDS             complement(8106..>8486)
                     /gene="traE"
                     /locus_tag="ACHUPM_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFR0871003.1"
                     /translation="NSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRPGAQN
                     QMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTDIKHY
                     ILILKRENGVTWLDNFGETDDEKK"
BASE COUNT         1985 a         2324 c         2027 g         2150 t
ORIGIN      
        1 ccctccctgc gtttctgctg cacaaactca aaatccgggc cgtcagagct gtacatggcg
       61 cggcttaacg ggtccacaaa cagacggtgc caggaggaat ggttttccac ctgcagcagg
      121 aaggaactga accactggtc tttggccgca ccaaacttgc caatcatgtc gcgctgcagt
      181 ggctggaact gatccgggaa taactggtta tatttcgcga actccttcgc actctgtttg
      241 aggataattt tgtaggagga gttaccccat gccgcacggg ccgcactgga ggccttgtca
      301 gagtcaaagt cgacgatgtt ctgtgtgatg gtgatatagg caccggtatg gcggcgacag
      361 gtgcggtaac ctttttgaat aaactcaccg accttgcgat ttttgaagtc cagcagacgc
      421 cagccttcat caatgacgtt cagtttcttg agagtacgcg gcgtgcggta catcctgttc
      481 tcgatgtaga taatcaggga gaacatcacc gcaaccagca gtgacggacg gtcttccagt
      541 ccgcccagct ccagcaccac cattttcgca tcatcccgca gggacggctc atcggagtta
      601 aaatactggc cgtaagtgcc gttggcagtg tactggtcaa gcagcacaat catttcgtcc
      661 agacggctgc ggatggtcgg tgacccggca tactgctcgc tgtcactggc gtttttcagg
      721 aaatccacca cgtcatcaat acgtgccctg ttctctttgg ccagccagga ggccctgacc
      781 gcctgcagca gcagaccttc atgcacttca tccaggttac cgttggggct ggccatcact
      841 gacaactggt cacggacacg ctccgctgac tggtcaatat cggtgatgtt cgcaaacggg
      901 ttaaaacgca gggtttcacc gtccagatat acgcccccca tgttctcaca cagggacttg
      961 tagccatccc ccatgtcgaa caccacggca aagcccccgg agtccagcac gctgcggata
     1021 agtggctgta tcagtccggt tttaccggcc ccggaggtgc cacagaccgc catgttgtag
     1081 ttggtgttat tcatcccctt aaagaagata tcgataaacg ccagctggtt acggtaggtg
     1141 ggcgccagca gacctgccgg tgtcaggggg ttatccgcca ctaacggcat caggttggcc
     1201 acattaaagc tctctgcgcg ctgaaccaca ccggcctctt tcagctgttt aaacagccct
     1261 ttcccggcca taaagggcag gcaggtcagg aaattgcgca tgtggttaaa gcgcggtgaa
     1321 atcagctcaa aaccgttttt acggaagctg ttcaggatgt cctgctccac ttccagtgcc
     1381 gtttcattat tgtccttgca gaaggctgtg atgttgagga agtaggacac gacagaagac
     1441 tggccggagc ccagccgctg gcgcagttcc ccccactcct tcgcctcttt ctccacggac
     1501 ggaaaccatt tggcatatga ggtcttcgac ttcttctcca ggtccatgta cttcaggttg
     1561 gcttcgctgt gggttttcac ctggtcttcc accaccagag tcagcgtcag gatgaacggg
     1621 caggaaatgg acatttccgg gttcagcaga ttgctgtagt tgtcggccat gttccacagg
     1681 aaggcgattt ccgggttacg ggccagatgg aaattcagga tgcgggccgt gctgttcctg
     1741 ccgttctcac gcaggcccag cgtcaggtaa tcagcccgga cattcaggtc aaaactgtcc
     1801 tccacacact gataattcag atcagaatac gggtccagct gacgtctttt cgggtacaga
     1861 gagtccggat tatggttaat catctccccg acaatctcga tgaaagcctg tgcatccact
     1921 gtctgtgtgg tgatttttgc gccgtgaaaa gaggcacgga tgattttcac cagattttcc
     1981 atttccagaa tgtcagcccg gctttttttc ttcgagggag aacagtacga gataaacacc
     2041 cggtaatgac gcagggtcag gggcagattc atcccctccg gcagcgggaa ctgtgtcgct
     2101 gccgctttca tataataggc acgggtaatg gcgttaaacc gttcagcctg ttcacctgac
     2161 caggagaact cacgcagccc gtattcaatc ctgtcaccaa ccagctgact ggacatcaga
     2221 tggatacaca acggaatacc gcgcggcagt ttggtgcgca gcatgtgatc gagtgcctcc
     2281 acaatggact tattcgcccc attgatggga atggcttcca gcataaagcc catcgtggtg
     2341 tcattcatga acagaccgga ttcctggttg taatcacggt acggcagcag acggctgaac
     2401 tgcgggaagc tcatttcgcc cagaacttca ttggcctttg cggattcgtc aggcagtttc
     2461 agtgctgtga cgagggagtt aaccgcctga gtgacggcct caagtgggtt attactcata
     2521 tgagtattcc ttagtgcgtt taaaaaagga aaatatcagt ctgtggccgt ggatatatca
     2581 cgcagtgcca ttcctgaaac cacagcacaa ataagagatg gaggctttta acaggaaacc
     2641 aggattaacg ttcctttact gaatgagcat catttaatgc ctgaatttgt ttttcgcgaa
     2701 tcttattgcg ctcattttct cttacccgtt tcacctctgc catttcgttt ctttttctgc
     2761 attctgatgc gattttattt gccttaccaa gtgttaaagg ttcagactga taattaagtg
     2821 tctcctgaaa tgcttctgct gcacagggag tttcctgtaa aatctctgtc agttgttctg
     2881 gtgttgcagg ttgttgcgca aggtaaacga tgtaaaaagg aatacataca atcatagcca
     2941 taacgccaac aaatataaaa gccagaacag attttaaata tatcgacttc atttcatttt
     3001 ccttcgaagc agtaagcact cattgcccac caccatttag tgtttccatc acgctgttga
     3061 aaggttcatt tgttttaact tcaatacact cccctgtatt tccaaagccc ctgccgccat
     3121 gtgcttcgat tgtccgaaga acctgcacgc atacgtccgt atttggcatg ggtgtaatat
     3181 tccagcttcc gccaccgtta atatttttta tcagcacaag aaaaataaag gtatgcattc
     3241 tttattatct ccgcttgtat attaatcact gaatatccct tcagttaact tatgaggtca
     3301 taaaacactg cagattttca ttcagattta ctctcttcag caaactttac tctcttcagc
     3361 aaacttccgg atggtatcct gttcacgctg acgggtggca gactcctgca ttgcatccac
     3421 tttctcctgc cagagacgcc ggatatcctt ttcatcccga ccagtcagtt ttttaccgga
     3481 cagcagacgg ttaagcagtt cctgtctcgc atccggtacg tcttcagtca gacgcataag
     3541 ctgaagcagg ctctgacggc tgagaaccat gagtgattcc ggcaggaggg tgaagaagaa
     3601 aataaaaaag cataccgcac cacagcaggc aaggaccgca aacaggattt tcagatatat
     3661 accatcaacg ccgaacgcag caataataca gggaatccat gcaatacacg cacaatacgc
     3721 caggatgtta tctattcggt tatgtctgcg atatatctgt tgatgttcat ccagaataga
     3781 atctattttt tcagcttctg atttatgcat agtgtttcct ctgcgtttcc tgccagagac
     3841 gccggatatc ctgttcatcc cgaccagtca gttttttacc ggacaacaaa cggttaagca
     3901 gttcctgtct cacatctggt acgtcttcag tcagacgcat aagccggagt aattcctgac
     3961 gactgagtac catgcgtgat tcctccggta cgctgtaata attaaaaata cgactgaata
     4021 ccccacaaca gcaggcaacg gacaggcaca gggatttcag atacaggcca gaaacacaga
     4081 atgtggcaac accacaaaga atagctataa ctaaaaatcc cttacccagt gccatacaca
     4141 aagaaccctg ttttctggat atccgctcat gctcatccag aataaaatct attttttcat
     4201 tttctgattt atgcatagtg tttcctctgc ctttcctgat acgcctgaca ttcaacgcac
     4261 aacgtcacac cgggaaatat tttccgccgg gcttccggaa taggatttcc gcatgcttca
     4321 cagagataaa caggaatacc atgaacattt atttcctggc gtatccggtt tattcctgtc
     4381 atggtcagtt gttctgtcac tgaatatgct tcatcggctt catcactcac ggaatattcc
     4441 tcccgacatg ttctgacacg ctaccgccca tcccttttca cgcacagggc actcgtttta
     4501 caggcggatt ttgaatacag aaagcccgcc ggcattgccg gctgatatac aacttcaggg
     4561 ccagttaatt aatacgtggt tttccccacg cagacggttt aataacaaaa aacacgctgg
     4621 atggctgatg ataaacatcc tggttatcaa tataaggcgc tatccataat gctgccgtct
     4681 gctcacccgt tcttagcgga cgaggaggtg ttaccggcgt aactgaactg accggaacaa
     4741 ccgttttcac ttcccgggca gcggtaaaca gcgggcgtgg tgccagcagt ttctgctccg
     4801 gatgtgctgt caccgcaggt ctgctgccgg acggagtggt cgcggtgacg gtctgcaccg
     4861 gcattgtccg gaagttccct tcagccaggc gcggcagtga tgccgcaacc ggctttgctt
     4921 ctgaggactg ctccagtttt ttggccttct cattggcctg ctccatcgtc atacaggtat
     4981 cggatgtggt ggcgttacac tcaaattccg tatttgttcc tgcgcagccg gaaagtaata
     5041 aggttcccag cagaggaata aataaagaaa tctgtttcat tgcccttctc cttttttatt
     5101 actccccaca acagcagaaa ttaactctgc tgtcagattt gttttccctg cacagggatg
     5161 tccggagacc actgtatttc ccggagaaga aaataagctc atcgacattc ccttcttcat
     5221 cacaccgaca gcagccatat taaatttgtg cggcaatgat tcagaactga acaccttcgg
     5281 agcaggaaga actatttttg tggtgcagtt actgagaaca gcatgcataa aatcatctga
     5341 tacgttttta tccggcacaa cgcgggctgt aataacatta atattacgca tgttcatttt
     5401 tatctccgtt gcttctgttt gttcggtcat cagggaaata aacatggcat ttcctgattt
     5461 ttcccggcag ggaagcgtct tccgtataaa cgtccttcag atattcatct gcggttacca
     5521 cccggataca tttcattttt ccgggcagca ccggatattt cccggtgatc gctgccagcg
     5581 agacatacat cagtgccagt gttgcccata atgcggtgac cagttgcatc agtgaccggt
     5641 cagtctccct gacataatct gcagcatcag ccgcgcggta atggcagata tcctgtattt
     5701 ctgatctgcc tgaagaatca gaggaagaaa accagcattc actgaccggg aactgtccgg
     5761 caggtgctgt ttgtcttacg aagttaatct ccttaaccag taatttcccc ggcgtggtgt
     5821 tgtccttcca taaaacaaac agagccatca gggccattgt ggccagtggc agaatgacgg
     5881 tgtacttcag acaccagaac agtcctcgca gaacccgggc gacaacatac cgggcggcat
     5941 ggcacgcctg acgggacgac atattatttg ccatcgttgc ctcctccctg gctgccggac
     6001 ataaactgct gcggattcag gttgcccagc tgcttcagca tctggtcagt attaaagccg
     6061 ttaagatgac tttcagctga tggcggaaca ggaaccggac tttccggatt atcttcttcc
     6121 gctctgttct gcgtgcgctc cagcgccatc tcttccacgg ttttcagctg gaagccgtcc
     6181 tggaacacca cggtcacttc gttgcccgca ccaatcggta tcaccgggtg atactgttcg
     6241 gcacgtttga tgtagtagtc actgagcgtc tgtgcggctt tcgatgtgcc gccaccgata
     6301 cccattttca ggacatcacc agccccgtaa gcggctgtgg cacccagccc gacagccggc
     6361 tgggaggcgc gctccattcc ctgaccgata ccgtcaacaa atcccgcgcc ccatgcccag
     6421 ccgaggattt tgccgttacg catcaccact tcgcccttga taccgttttt accccggaag
     6481 ctgacatgcc ccttaatcgg catatcaata gttttgccgt ccttcaggca actgatattg
     6541 cgggtgcgta caatggcacg ctcactggac acatcccccc aggcttccag ccccacaaaa
     6601 catcccgttg cgtcatacgt cttgctgttc ggcatttcca ccaggccggt gatacgcaac
     6661 tgcatcggca ccgtggattc attaccggtg actgaggcat tggcatccgc cccttcaatc
     6721 agcatggctt tcgcaaaact tcctgacgga atgtacggca gcgatggtcc ctgttttccc
     6781 tcattacggg taaacacctt acgctgtatc cggttaggca ccggcacaga ctggtacgtc
     6841 acctgtggtg gtggcgtgac accattcccg ggataaaacg ccgtcggcgg tggaacagca
     6901 acgctgccct gcggcgggaa ggatacgggg gtgtttcctg gctgtggttc gccttccggc
     6961 ccgggcggtg aggcaggcat ctgaggtaca ggctcaccgg tcgccgtgac gggattagca
     7021 cccagggctt ttacctgctc tgccagggcg gcattgtcct gtcccagctt ttcaatacga
     7081 cgctggtcat caccgcgctg tttgttcagg acatccagtt cacgacggat ttcctcatac
     7141 tgcttctgca tctgcgctgc cgtcacctgc atctctgtgg tggcatgctg acgcaccttg
     7201 tcatcaaagg tcgtatccac cacacctgtc atatccggca caggctcctg ttcagccacc
     7261 gcctcaccgt taccggacat gtccacatca gacagataca gtaccccacc aatcgcggag
     7321 gctgcaccga caaccacaat ccccagccac aggtactgct tgcgtttcac aatggtattg
     7381 atactggcca tcagttgccc tccccgtcac ggatgacata cacatccatg cgcgcacctg
     7441 ccagtaactg gctggcaggc tggctgaaca tcacggcacg ggttcccggc tgccagaagt
     7501 cactttcccg gatattcagg gcagacagcg ttttattttc cacggcaaag cggaccatct
     7561 tcaggtgatt ccccgtccat acggcatctg ccactgaaga cagccccgcc ggggcctgca
     7621 gggtttcctt tgtcacagga acctgatacc agcctgcagg taattttccg ccacggacag
     7681 cctggctgat ggtcacaagc agggattcgt aaggcgtgga cgtttcccac gctccggctt
     7741 cttctccggt tccgcgcagg tcactgacca gctgaatggt acgccccgcg ccttcacggg
     7801 gaacagcctg aatggaaagg ttcagaccac gctctgtttc cagaatgaac gtaaagggct
     7861 ttttgttgac ggtggccacc accacaccgc cggaggcggt ctgctcatta ttggtcaggg
     7921 caccatccag actgttcacg gcgataatcc ggtcaccggg aacggtaaac agattcgggc
     7981 tggtattgct gatggcaata ctggcctgac caccgttcac cattggcacc acagtggggg
     8041 ccagcgtacc gtttgcggcc atcacgctgc aggaaaaaaa caggccgccg aatattattg
     8101 ccgtattatt ttttctcatc gtctgtttcc ccgaaattat ccagccaggt caccccgttt
     8161 tcccgcttca gaataaggat gtaatgtttg atatcagtaa aaggttcgga gttaccaatc
     8221 catgttttcc gaattccacg gatttccaca cgaccatact gaggccagac acggacactg
     8281 gtctggaaaa aagcagaatt cacgttatct tttttaatga gtttcgcttc ttcagccaga
     8341 ataactttca tctggttctg tgcacccggg cggatatatt gcagaagcgc ctgatgtgaa
     8401 gcatcgacga tttccggtga gacattcaga cggagggcaa taaatgacag cgccatctgc
     8461 tgcaaataag aggcatcggc actgtt
//
LOCUS       DAZPAC010000173         8406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZPAC010000173 DAZPAC010000000
VERSION     DAZPAC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.173"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            85..1581
                     /gene="ravA"
                     /locus_tag="ACHUPM_003311"
     CDS             85..1581
                     /gene="ravA"
                     /locus_tag="ACHUPM_003311"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR0871004.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
     gene            1575..3026
                     /gene="viaA"
                     /locus_tag="ACHUPM_003312"
     CDS             1575..3026
                     /gene="viaA"
                     /locus_tag="ACHUPM_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR0871005.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3031..4023)
                     /gene="asnA"
                     /locus_tag="ACHUPM_003313"
     CDS             complement(3031..4023)
                     /gene="asnA"
                     /locus_tag="ACHUPM_003313"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR0871006.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            4175..4633
                     /gene="asnC"
                     /locus_tag="ACHUPM_003314"
     CDS             4175..4633
                     /gene="asnC"
                     /locus_tag="ACHUPM_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR0871007.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4723..5166
                     /gene="mioC"
                     /locus_tag="ACHUPM_003315"
     CDS             4723..5166
                     /gene="mioC"
                     /locus_tag="ACHUPM_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR0871008.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5545..7434
                     /gene="mnmG"
                     /locus_tag="ACHUPM_003316"
     CDS             5545..7434
                     /gene="mnmG"
                     /locus_tag="ACHUPM_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR0871009.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7498..8121
                     /gene="rsmG"
                     /locus_tag="ACHUPM_003317"
     CDS             7498..8121
                     /gene="rsmG"
                     /locus_tag="ACHUPM_003317"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR0871010.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         2131 a         2121 c         2128 g         2026 t
ORIGIN      
        1 ctttgagact attttagcca ccagcaagaa acgtctatac tcgcaattta cgcagaactt
       61 ttgacgaaag gacgccactt cattatggct caccctcatt tattagcgga aagaatttcc
      121 cgcctgagca gttcgttgga aaaggggctt tatgaacgta gccacgccat ccgcttgtgt
      181 ttattagcgg cattaagtgg tgaaagtgtg ttcctccttg gcccgccagg tattgccaaa
      241 agtttgatcg cccggcgctt aaaattcgcc tttcagaatg cccgcgcgtt tgaatatctg
      301 atgacccgct tctccacgcc ggaagaagtt tttggtcccc tttctattca ggcgctaaaa
      361 gatgaagggc gctatgaacg tttaaccagc ggttacctgc cggaagcaga aattgtcttt
      421 ctggatgaga tctggaaagc gggcccggca attcttaata ccttgctcac cgccattaac
      481 gagcgccagt tccgcaacgg tgcacacgta gaaaaaatcc cgatgcgtct gctggtggcg
      541 gcctccaacg agctgccgga agcagacagc agtctggaag cgttatatga ccgcatgctg
      601 attcgtctgt ggttagataa agtgcaggat aaagcgaatt tccgctccat gctgaccagt
      661 caacaggatg aaaacgacaa tccggttcct gatgccttgc aggtcacaga tgaagaatat
      721 gaacgctggc agaaagagat tggtgaaatt acgctgcccg atcatgtatt tgagctgatt
      781 tttatgctgc gcctgcaact ggataaatta ccggatgcgc cttatgtctc ggatcgtcgc
      841 tggaaaaaag cgatccgatt attgcaggcc agcgcctttt ttagcggtcg cagtgctgtt
      901 gccccggtag atctcatttt gctgaaagat tgcctgtggt atgacgcgca aagcctgaat
      961 ttgatacaac aacaaattga tgtattgatg accggtcatg cctggcaaca gcaagggatg
     1021 ttgactcgcc ttggcgcgat tgtgcaacgt cacctgcaac tccagcagca acaaagcgat
     1081 aaaacagcct taacggtaat tcgtctgggc ggcattttca gccgtcgtca gcagtatcaa
     1141 cttcctgtta acgttactgc ttccactctg actctactgc tgcaaaaacc gttaaaactg
     1201 catgatatgg aagtggttca tatctccttt gagcgtagcg cgctggaaca gtggctgagc
     1261 aaaggtggtg aaattcgcgg taaactgaac ggtatcggct ttgcccagaa actgaatctg
     1321 gaagttgata gcgcccaaca tcttgtcgtt cgcgatgtaa gtttacaagg cagtacgctg
     1381 gcacttcccg gttcatcggc tgaaggtctg cctggtgaaa taaaacaaca actggaagag
     1441 cttgaaagcg actggcgcaa gcaacacgct ttattcagcg aacagcaaaa atgtctgttt
     1501 atccctggcg actggttagg tcgcattgaa gccagcctac aggatgtcgg tgcacagatt
     1561 caccaggcac aacaatgcta acgctggata cgcttaatgt gatgctggcc gtcagcgaag
     1621 agggattgat cgaagagatg atcatcgcac tgctggcctc accgcagctg gcggtcttct
     1681 ttgaaaaatt cccacgcctg aaggcggcaa tcactgatga tgttccccgc tggcgtgagg
     1741 cgctgcgcag tcggctgaaa gatgcccgag tcccgccgga actcaccgaa gaggtgatgt
     1801 gctatcagca aagccagctc ctctccacgc cgcagtttat tgtgcagcta ccacagatcc
     1861 tggacttact gcatcgtctg aattccccat gggcagaaca agcccggcag ttggttgatg
     1921 ctaacagcac aataacttca gcgttacaca cgctttttct ccagcgttgg cgtttaagtc
     1981 tgatcgtgca agcaacgacg ttaaatcaac agctattaga agaagaacgc gaacaactgt
     2041 tgagtgaagt tcaagaacgc atgacgctga gcggacaact tgaaccgatt ctcgcagata
     2101 acaatactgc agctggtcgt ctgtgggata tgagcgccgg tcagcttaaa cgtggcgact
     2161 atcagttgat tgtgaaatac ggtgaatttc ttaacgaaca gccggaactg aaacgcctgg
     2221 cagagcagct ggggcgttct cgggaagcca aatcaatacc gcgcaacgat gcgcagatgg
     2281 aaaccttccg caccatggtg cgcgaaccgg cgacggttcc tgagcaggtt gatggtctgc
     2341 aacaaagcga tgatatttta cgtcttctgc cgccagaact ggcgacacta gggataacgg
     2401 aactggagta tgagttttac cgtcggctgg tggaaaaaca gttgctcacc tatcgcctgc
     2461 acggtgagtc gtggcgtgaa aaagtgatcg aacgtccggt ggtacataaa gattacgatg
     2521 aacagccgcg cgggccgttt attgtctgtg tggatacttc cggctcaatg ggcggcttta
     2581 atgaacagtg tgcgaaagcg ttctgcctgg ccttgatgcg cattgctctc gctgaaaacc
     2641 ggcgctgcta tattatgcta ttttccaccg agatcgtccg ttatgagctt tcaggccccc
     2701 aaggcatcga acaagcaatc cgttttttaa gccagcagtt tcgtggcggc accgatcttg
     2761 ccagttgttt tcgcgccatt atggaacgct tgcaaagcag ggaatggttt gatgccgatg
     2821 cggtggtgat ttctgatttt atcgctcagc ggttgcctga cgacgtgacg agtaaagtga
     2881 aagagctgca gcgggtacat cagcatcgct ttcatgccgt ggcgatgtcg gcacacggca
     2941 aacccggcat catgcgcatt ttcgatcata tctggcgctt tgataccggg atgcgaagcc
     3001 gcctgctcag acgctggcgg cgataaatta ttacagcaga gaagggacgt tctcgcgaac
     3061 agcagctggc catacaccac actgaacctg gccgatatgc ggcagttgca gcagcagcat
     3121 ggtcaaacga gactggccga taccgccgcc gatggtctgc ggcatttcac cgcgcagcag
     3181 cgcctgatgc cattccagct gcagacgatc ttcatcaccg gtcagcgcca actgatgctt
     3241 caacgtgtcg gcatccacgc ggatccccat ggaagaaagc tcaaacgcat cttccagtac
     3301 agggttccac accagaatat cgccgttcag acccgcatga cccagctctg acggggtgct
     3361 ccagtcatca taatccggtg cgcgcacgtc gtggcgatga ccatcgctca gcttgccgcc
     3421 aatcccgaca aggaataccg cgccaagatc tttcgctatc gcccgctcac gccctttggc
     3481 atcgagatcc ggataacgag acagtaactc ctggctgtgt acgaagtgga tctgatccgg
     3541 caggaacggt gccaggccaa actcttcgct aaccgcagct tcggttgctt taattcccgc
     3601 ccagatcgcc tctaccgtgc ttttcagagt cgagaattga cgctcaccgt cgcccattac
     3661 gcgttcccag tcccactggt caacatagac cgagtgcaac ggagaaagac ggtcttcatc
     3721 ggggcgaagg gctttcatgt gcgtgtacag cccttcgccc gcgctgaagt cgtgttgccc
     3781 taaggtctga cgtttccact tcgccagtga atgaaccact tcgaactggg catcaggcag
     3841 agctttcact tttacctgca ccgctttttc acagcccgac aagttatcct gcgtgccatc
     3901 ccccacacgg ctaagaatcg gcgcctggac ttcgatcagc cccagacgtt cttccagttg
     3961 gcgagaaaag tgagatttca cgaagctaat ttggcgttgt ttggcaatgt aagcggtttt
     4021 cattttttat actcctgcgt cctgttgctt atgattaagc aacaaaaacc gacaaacatg
     4081 caataatcat tcaataaaaa gcctaacaca cttttgattc attaaaaaag aaggctaaaa
     4141 tagaatgaat catcaatctg cataagaaaa tcctatggaa aattatctga tcgacaatct
     4201 ggaccgtggc atcctggaag cattaatggg caatgcgcgc accgcttacg ccgaactggc
     4261 gaaacaattt ggcgtcagtc cggggacgat tcacgttcga gtagagaaaa tgaagcaggc
     4321 ggggatcatt accggggcgc gtattgatgt cagcccgaag cagctcggtt atgacgtagg
     4381 ctgctttatc ggcattatat taaagagcgc caaagactac ccttccgcgc tggcaaagct
     4441 ggaaagcctt gctgaagtca ctgaagccta ttacacaaca ggccactaca gcatctttat
     4501 aaaagtaatg tgccgttcga tcgacgctct ccagcatgta cttatcaaca agatccaaac
     4561 aattgatgaa attcagtcca ccgagacatt gatcgtcctg caaaacccga tcatgcgtac
     4621 catcaagcca taatcggctt ttttaatccc atacttttcc acaggtagat cccaacgcgt
     4681 tcacagcgta caatacgcca ctcttaataa aggtggcggt ttatggcaga tatcactctt
     4741 atcagcggca gcaccctcgg cggtgccgaa tatgtagcag aacacctggc tgaaaagctg
     4801 gaagaggcgg gttttaccac cgaaacgctg cacggtccgc tgttggaaga tttatctgcc
     4861 tcggggatct ggctgattat cagctccacc cacggtgccg gagatattcc ggacaacctt
     4921 tctcctttct atgaagcatt gcaggaacag aagcccgatc tttctgcggt ccgctttggc
     4981 gcaatcggta ttggtagtcg tgaatatgac accttttgtg gggctatcga taaactcgag
     5041 gcagaactca aaaattccgg tgcaaaacag acaggcgaaa cactgaagat caacattctt
     5101 gatcacgaca ttccggaaga tccggcagaa gaatggctgg gatcgtggat taatttactc
     5161 aaataagtat acagatcgtg cgatctactg tggataactc tgtcaggaag cttggatcaa
     5221 ccggtagtta tccaaggaac aacggttgtt cagtttttga gttgtgtata acccctcatt
     5281 ctgatcccag cttatacggt ccaggatcac cgatcattca cagttaatga tcctttccag
     5341 gttgttgatc ttaaaagccg gatccttgtt atccacaggg tagtgcgatc ctaataagag
     5401 atcacaatag aacagatctc taaataaata gatcttcttt ttaataccca ggatcccagg
     5461 tctttctcaa gccgacaaag ttgagtagaa tccacggccc gggcttcaat ccattttcat
     5521 accgcgttat gcgaggcaat caccatgttt tatccggatc cttttgacgt catcatcatt
     5581 ggcgggggtc atgcaggcac cgaggccgcg atggccgcgg cgcgtatggg tcaacagact
     5641 ctgcttttga cacacaatat cgacactctg gggcagatga gctgcaaccc ggcgatcggc
     5701 ggtattggga agggacatct ggtaaaagaa gtggatgcac tcggcggtct gatggcgaaa
     5761 gcgatcgatc aagcgggtat ccagtttagg atactaaacg caagcaaagg accggcggtt
     5821 cgcgctaccc gagctcaggc ggatcgtgtg ctctaccgtc aggcggtacg tacggcgctg
     5881 gagaaccaac cgaacctgat gatcttccag caggcggttg aagatcttat tgtcgaaaac
     5941 gatcgcgtgg tcggtgctgt tacccaaatg ggactgaagt tccgtgccaa agccgtcgtg
     6001 ctcaccgttg ggacgttcct cgacggtaaa attcatatcg gtctggataa ttacagcggt
     6061 ggtcgtgctg gtgatccgcc gtccattccg ctttctcgcc gtttgcgtga actgccgctg
     6121 cgcgttggtc gtctgaaaac cgggacacca ccgcgtattg atgctcgaac tatcgacttt
     6181 agtgtgctgg cgcaacagca tggcgataac ccaatgccgg tattctcgtt tatgggcaat
     6241 gcgtcccagc atccccagca ggtgccgtgt tatatcactc ataccaacga gaaaacccat
     6301 gatgtgatcc gcagtaacct cgatcgtagc ccaatgtacg caggggtgat cgaaggtgtc
     6361 ggcccacgct actgcccatc gatcgaagac aaagtcatgc gcttcgccga cagaaatcag
     6421 catcagatct tccttgaacc ggaagggctg acctctaacg aaatttatcc gaacggtatc
     6481 tccaccagcc tgccgttcga tgtgcagatg caaatcgtcc gctccatgca ggggatggaa
     6541 aacgcgaaga tcgtgcgtcc gggttatgcc attgagtatg acttcttcga tccacgcgac
     6601 ctgaaaccga cgctggagag caagtttatc caggggctgt tctttgctgg tcagattaac
     6661 ggcactaccg gttacgaaga agccgctgcg caaggtttgc tggtcggtct taacgctgcc
     6721 cgtctgtctg ctgacaaaga aggttgggct ccggcgcgtt ctcaggcgta tctcggcgta
     6781 ctggttgatg acctgtgcac tttaggaacc aaagaaccgt atcgtatgtt tacttcgcgc
     6841 gcagaatatc gtctgatgct gcgcgaagat aatgcggatc tgcgtttgac tgaaatcggt
     6901 cgtgaactgg gcctggtgga tgacgaacgt tgggcgcgct ttaacgagaa acttgagaat
     6961 atcgagcgtg agcgtcagcg tctgaaatcg acctgggtaa ccccgtcggc ggaagctgca
     7021 gccgaagtga atgctcacct gactgcgccg ctttcccgtg aagccagtgg tgaagatctg
     7081 ctgcgtcgtc cggaaatgac ttatgaaaaa ttaaccacgc tgacgccgtt tgcccctgcg
     7141 ttgacagacg aacaggcggc ggaacaggtt gagattcagg ttaaatacga aggttatatc
     7201 gcgcgccagc aagatgagat cgaaaagcag ctgcgtaacg agaacaccct gctacccgcg
     7261 acactggatt accgccaggt atccggtctt tctaacgaag tgatcgccaa acttaacgat
     7321 cacaaaccgg cctctatcgg ccaggcttcg cgtatttctg gcgtcacgcc tgcggctatc
     7381 tccattctgc tggtgtggct gaaaaaacag ggtatgctgc gtcgtagcgc ataacgcatt
     7441 aaaaatgcct ggtaagcacc cgcttaccag gcaacgcatc aagaacaggt aatcaccgtg
     7501 ctcaacaaac tctccttact gctgaaagac gcaggtattt cgcttaccga tcaccagaaa
     7561 aaccagctta ttgcctacgt gaatatgctg cataaatgga acaaagcgta caacctgact
     7621 tcggtccgcg atcctaatga gatgctggta cgccatattc tcgatagcat tgtggtggca
     7681 ccgtatctgc aaggtgaacg gtttatcgat gtcggcaccg gaccaggact gccaggcatt
     7741 ccactctcta tcgtgcgtcc tgaagcccat ttcactctgt tggatagcct tggtaaacgc
     7801 gtgcgtttcc ttcgtcaggt gcaacatgag cttaaactgg agaatattga gccagtacag
     7861 agcagggtag aagagtttcc ttcagagccg ccatttgatg gcgtaattag ccgcgctttt
     7921 gcctctctga acgatatggt gagctggtgc caccatcttc ctggtgagca aggccgtttc
     7981 tacgcgctga aagggcaaat gccggaagat gaaatcgctt tgttgcccga agaatatcag
     8041 gtcgaatcag tggttaaact tcaggttcca gctctggatg gcgaacgtca tctggtggtg
     8101 attaaagcaa ataaaattta atttttatca aaaaaatcat aaaaaattga ccggttagac
     8161 tgttaacaac aaccaagttt tctactgata taactggtta catttaacgc cacgttcact
     8221 cttttgtatc aacaagataa cgtggctttt tttggtaagc agaaaataag tcattagtga
     8281 aaatatcagt ctgctaaaaa tcggcgctaa gaaccatcat tggctgttaa aacattatta
     8341 aaaatgtcaa tgggtggttt ttgttgtgta aatgtcattt attaaaacag tatctgtttt
     8401 tagact
//
LOCUS       DAZPAC010000174         8401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZPAC010000174 DAZPAC010000000
VERSION     DAZPAC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.174"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACHUPM_003318"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACHUPM_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR0871011.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(1360..1518)
                     /locus_tag="ACHUPM_003319"
     CDS             complement(1360..1518)
                     /locus_tag="ACHUPM_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0871012.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            2089..2238
                     /locus_tag="ACHUPM_003320"
     CDS             2089..2238
                     /locus_tag="ACHUPM_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871013.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3005..3445)
                     /locus_tag="ACHUPM_003321"
     CDS             complement(3005..3445)
                     /locus_tag="ACHUPM_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR0871014.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3663..4052
                     /locus_tag="ACHUPM_003322"
     CDS             3663..4052
                     /locus_tag="ACHUPM_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871015.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4096..4284
                     /locus_tag="ACHUPM_003323"
                     /pseudo
     CDS             4096..4284
                     /locus_tag="ACHUPM_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4251..5450)
                     /gene="tet(A)"
                     /locus_tag="ACHUPM_003324"
     CDS             complement(4251..5450)
                     /gene="tet(A)"
                     /locus_tag="ACHUPM_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFR0871016.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            5556..6206
                     /gene="tetR(A)"
                     /locus_tag="ACHUPM_003325"
     CDS             5556..6206
                     /gene="tetR(A)"
                     /locus_tag="ACHUPM_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFR0871017.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(6238..6480)
                     /locus_tag="ACHUPM_003326"
     CDS             complement(6238..6480)
                     /locus_tag="ACHUPM_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFR0871018.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(6786..7622)
                     /locus_tag="ACHUPM_003327"
     CDS             complement(6786..7622)
                     /locus_tag="ACHUPM_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR0871019.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(7622..>8401)
                     /locus_tag="ACHUPM_003328"
                     /note="aph(3'')-Ib"
     CDS             complement(7622..>8401)
                     /locus_tag="ACHUPM_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFR0871020.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1889 a         2322 c         2332 g         1858 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttatacatca cttttacatc attccttcag
     2641 ctcatacgtc actttcatcc tctgtgccat ttgtacatca cccccttcct gaacgtgctg
     2701 acagcgggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc
     2761 gttcaccgtt ttcggatggg aagaacgccg gaaacaggac ttaccccggc tgaatggtgt
     2821 gaatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgcg
     2881 cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg
     2941 ccctgagggc ctctcaggcg gtgctgataa cagatgttat ggtaaatcag agtcacctgc
     3001 actgttactc tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt ttttcctgtg
     3061 ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg ttccctctcc tcatttcgca
     3121 ggcggttttc tgcttcccgc gccatcttgt cttcaagcat tagggtaaca gtctgctgca
     3181 gagcgtccag tttctgctga atgacgtcta gctgagcatt ttccgcctgg ctaacgacag
     3241 atttggagtg tccctccttg tgcctttttg gtgtctcaga agtgcgcagc tcgccatagg
     3301 cccgaatcag ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg cgatagctga
     3361 caaggccttt agccatgtca cgatataatt gcgagcgcga ctttcctgtt aatttcagtg
     3421 tctcttgaac tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt accgtattgt
     3481 aacagcagaa catattactg ttttaacaca aaaggtctga ggctgcccgt gaggcgtgaa
     3541 aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt cgaatactgt
     3601 atataaacac attgttataa atacagtttt tgctgttttt aaaagtcaaa gaggataatt
     3661 ttatgagtac agagttaaat agcggtactg tcggtggttt tggtcacatg ggaccaaatg
     3721 acccctacaa tgatggtcat agcggctctg gaagttcggg aggtagtttt aacggtggtg
     3781 gtaatggtgg ccatgataat agtagtggtt catcttatac tgcatccttt ggtctcatca
     3841 ctggccgaca cccgataact gtcccaggta cgaagaatat taattctgtc gatctttctg
     3901 ctgtcattaa taacattata catcatgaag gtgcacgcaa taacatgtac ctagatactg
     3961 cgaacaatgt aagagtggga gtgacgggca ctggctggca atgtctagca acggcaggca
     4021 tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt
     4081 gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact
     4141 ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat
     4201 tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa
     4261 cgggaatttg aagaatttct ccaaagcccg cgacgcagcg ccggcaggca gagcaagtag
     4321 agggcagcgc ctgcaatcca tgcccacccg ttccacgttg ttatagaagc cgcatagatc
     4381 gccgtgaaga ggaggggtcc gacgatcgag gtcaggctgg tgagcgccgc cagtgagcct
     4441 tgcagctgcc cctgacgttc ctcatccacc tgcctggaca acattgcttg cagcgccggc
     4501 attccgatgc cacccgaagc aagcaggacc atgatcggga acgccatcca tccccgtgtc
     4561 gcgaaggcaa gcaggatgta gcctgtgccg tcggcaatca ttccgagcat gagtgcccgc
     4621 ctttcgccga gccgggcggc tacagggccg gtgatcattg cctgggcgag tgaatgcaga
     4681 atgccaaatg cggcaagcga aatgccgatc gtggtcgcgt cccagtgaaa gcgatcctcg
     4741 ccgaaaatga cccaaagcgc ggccggcacc tgtccgacaa gttgcatgat gaagaagacc
     4801 gccatcaggg cggcgacgac ggtcatgccc cgggcccacc ggaacgaagc gagcgggttg
     4861 agagcctccc ggcgtaacgg ccggcgttcg cctttgtgcg actccggcaa aaggaaacag
     4921 cccgtcagga aattgaggcc gttcaaggct gccgcggcga agaacggagc gtggggggag
     4981 aaaccgccca tcagcccacc gagcacaggt cccgcgacca tcccgaaccc gaaacaggcg
     5041 ctcatgaagc cgaagtgccg cgcgcgctca tcgccatcag tgatatcggc aatataagcg
     5101 ccggctaccg ccccagtcgc cccggtgatg ccggccacga tccgcccgat atagagaacc
     5161 caaaggaaag gcgccgtcgc catgatggcg tagtcgacag cagcgccggc cagcgagacg
     5221 agcaagaccg gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg tgcgcaggca
     5281 aattgcatca acgcatacag cgccagcaga atgccatagt gggcggtgac gtcgttcgag
     5341 tgaaccagat cgcgcaggag gcccggcagc accggcataa tcaggccgat gccgacagcg
     5401 tcgagcgcga cagtgctcag aattacgatc aggggtctgt tgggtttcac gtctggcctc
     5461 cggaccagcc tccgctggtc cgattgaacg cgcggattct ttatcactga taagttggtg
     5521 gacatattat gtttatcagt gataaagtgt caagcatgac aaagttgcag ccgaatacag
     5581 tgatccgtgc cgccctggac ctgttgaacg aggtcggcgt agacggtctg acgacacgca
     5641 aactggcgga acggttgggg gttcagcagc cggcgcttta ctggcacttc aggaacaagc
     5701 gggcgctgct cgacgcactg gccgaagcca tgctggcgga gaatcatacg cattcggtgc
     5761 cgagagccga cgacgactgg cgctcatttc tgatcgggaa tgcccgcagc ttcaggcagg
     5821 cgctgctcgc ctaccgcgat ggcgcgcgca tccatgccgg cacgcgaccg ggcgcaccgc
     5881 agatggaaac ggccgacgcg cagcttcgct tcctctgcga ggcgggtttt tcggccgggg
     5941 acgccgtcaa tgcgctgatg acaatcagct acttcactgt tggggccgtg cttgaggagc
     6001 aggccggcga cagcgatgcc ggcgagcgcg gcggcaccgt tgaacaggct ccgctctcgc
     6061 cgctgttgcg ggccgcgata gacgccttcg acgaagccgg tccggacgca gcgttcgagc
     6121 agggactcgc ggtgattgtc gatggattgg cgaaaaggag gctcgttgtc aggaacgttg
     6181 aaggaccgag aaagggtgac gattgatcag gaccgctgcc ggagcgcaac ccactcacta
     6241 cagcagagcc atgtagacaa catcccctcc ccctttccac cgcgtcagac gcccgtagca
     6301 gcccgctacg ggctttttca tgccctgccc tagcgtccaa gcctcacggc cgcgctcggc
     6361 ctctctggcg gccttctggc gctcctgctg cggcgtccgc tcgtgggccg tggcgcgggt
     6421 ccgcgcgccg gcctcgtgcg cctggcgctc gcgggcgagg tccagggcgg ccgtcttcac
     6481 gttctgcctt gcgcagatga gatagatcta gcgtggactc aaggctctcg cgaatggctc
     6541 gcgttggaaa ctttcattga cacttgaggg gcaccgcagg gaaattctcg tccttgcgag
     6601 aaccggctat gtcgtgctgc gcatcgagcc tgcgcccttg gcttgtctcg cccctctccg
     6661 cgtcgctacg gggcttccag gggtcgtttg cgggaagggg cggaatccta cgctaaggct
     6721 ttggccagcg atattctccg gtgagattga tgtgttccca ggggatagga gaagtcgctt
     6781 gatatctagt atgacgtctg tcgcacctgc ttgatcgcgg ccgcgatagc tagatcgcgt
     6841 tgctcctctt ctccatccgc gttccaagct gcggaaaggc acccataagc gtacgcctgg
     6901 tcgagcaggc gacgcggatc gacgtccagc gcacgagaga atgcgtccgc catctgtgca
     6961 atgcgtctag gatcgagaca aaggtcgtct ctgtcagccg gatcgtagaa catattggcg
     7021 gcgccaaagc ccacttcacc gaccagaccg acgggatcta tcaccagcca gccgcgactg
     7081 gagaacatga tgttttcatg atgcagatcg ccatgtagcc cacgcagttc cgaggcattg
     7141 ctcatcattt gatcggctat aatcgccgcg tggacgtagt cagtttgaca acctgcgttt
     7201 tgatcatcgc gcgcccgctg aaacaaagct gcaaagcgat cccggatcgg gagaagggca
     7261 gaaggcaggg gttcctcaga tgcggcatac agcttcgcca ttagttccgc tgcaatttcg
     7321 gtcgcctggt agtcgccgtg ctcggcaacg atgtgagaga gcattcgctc cccggcatat
     7381 tcgagcaaca tcagattgtt ctcacgaccg agcaaccgga ctgctcccct cccattgcgc
     7441 cataccagat agtcggcccc gcgcagttca tcagcaatgt cttctatagg tttcaatccc
     7501 ttgacgattg caggagtccc gtctggcaat gaaactttcc aaacgaggct ggaaaaggtg
     7561 tccgcaatga gaacaggttg cgaaacgtgc caatgagcag gaaaaacagg cggcatgaac
     7621 atcaacccca agtcagaggg tccaatcgca gatagaaggc aaggcgttcg cggtcggggg
     7681 cttcgatccc caatacattg aataggacag cgaaggcgcg ctctgcttca tctggcgctg
     7741 cccagttctc ttcggcgtta gcaatcatga gtgccaaatc ggcatagcga tctgctgttc
     7801 cgagccgccc aaggtcgatc agacccgtgc attgaagagt tttagggtcc accatgaagt
     7861 tcggcatgca gggatcacca tggcaaacaa ccatatcggt gcgctcttgg tcgagccgca
     7921 ccggtagctc tcgttcgaca cgagccaaaa gatcgagctg cggcgtactc ttgtcctcgt
     7981 ccggtaagaa gtcgggattg acggcattgc gggacaccac atcaacggcg cgtccgaaca
     8041 ttcgcgacag cctgcgctca aactgacatt gatcaaccga taggctgtga acagcgccaa
     8101 gttgctgccc cattgacggc cacgctttga gcaaatccgc tccagacaga tcagccgccg
     8161 gtactcccgg aattgccgtt atcaccaagc atgcaccctc ctgttcctcc tgccagttga
     8221 tgacctcggg gcaagccaca cctcgacctt tgagccaaat gaggcggtca cgctctccag
     8281 cgagctcacc gcggcgggaa gcaggtgcga ttttcgcgaa ggcatgcccg tcaccacgtc
     8341 gaaaaacaaa atcaccagat tctccgcctc tgacaggcaa ccagtcagaa tgcgattcac
     8401 c
//
LOCUS       DAZPAC010000175         8384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZPAC010000175 DAZPAC010000000
VERSION     DAZPAC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.175"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            500..1771
                     /locus_tag="ACHUPM_003329"
     CDS             500..1771
                     /locus_tag="ACHUPM_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR0871021.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(1801..2805)
                     /gene="dppF"
                     /locus_tag="ACHUPM_003330"
     CDS             complement(1801..2805)
                     /gene="dppF"
                     /locus_tag="ACHUPM_003330"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR0871022.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2802..3785)
                     /gene="dppD"
                     /locus_tag="ACHUPM_003331"
     CDS             complement(2802..3785)
                     /gene="dppD"
                     /locus_tag="ACHUPM_003331"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR0871023.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(3796..4698)
                     /gene="dppC"
                     /locus_tag="ACHUPM_003332"
     CDS             complement(3796..4698)
                     /gene="dppC"
                     /locus_tag="ACHUPM_003332"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR0871024.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4708..5727)
                     /gene="dppB"
                     /locus_tag="ACHUPM_003333"
     CDS             complement(4708..5727)
                     /gene="dppB"
                     /locus_tag="ACHUPM_003333"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR0871025.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(6035..7642)
                     /gene="dppA"
                     /locus_tag="ACHUPM_003334"
     CDS             complement(6035..7642)
                     /gene="dppA"
                     /locus_tag="ACHUPM_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR0871026.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
BASE COUNT         1932 a         2277 c         2117 g         2058 t
ORIGIN      
        1 catgcccagc tctgcgagcg tcatatagcc gcctgtgttg taatgacaac gtttcgcggc
       61 tattcttgag tggtctaggt tcaagattag cccccgttat gttgtcaggt acatacctgc
      121 aacgtgcggg ggttttctct ctccagcaac caatgccacc agggataaag cccccgcaac
      181 attgcgcctc accggaaccc tttcggcttg ccgctgattt tacgattgct ttttactgtc
      241 agcacccggc aatcttcctc attgcgcgat gccttccagc ttaatttctt tatttgcaat
      301 agttgcagtt catatccggg ctgactccag actggtagtg aaatgcgcaa ttttgtagaa
      361 ttgcgcctcc tcgcgacctc cccagtgaat ctgttgcctc attttttaca tactgcgtat
      421 tcgacttctc cacctgttgc gcaagagaaa ctgggtttat tcatttttgc gaggccgact
      481 tctttctgga caggacttta tgcagcacaa cacactatcg aaacacaatc agaaattgcc
      541 gtttacacgc tacgacttcg gctgggtttt attatgcata ggcatggcga ttggtgccgg
      601 aaccgtgctg atgccagtac aaattggctt gaagggaatt tgggtattta ttaccgcagc
      661 gatcattgct tatcctgcca cctgggtagt gcaggacatt tatttaaaaa ccctttctga
      721 aagcgattcc tgtaatgact acaccgatat tatcagtcat tacctgggga agaactgggg
      781 aattttcctc ggggttatct actttttgat gattatccac gggattttta tctactctct
      841 ctccgtggtt ttcgacagcg cctcgtacct gaaaaccttc ggtttaaccg atgccgatct
      901 ttcacaatct ctactttata aagtcgctat tttcgccgta ctggtagcga ttgcgtctgg
      961 tggtgaacga ttactgttta agatttccgg gccaatggtg gtggtcaaag tagggattat
     1021 tgtcgtgttc ggttttgcga tgatcccgca ctggaatttc gccaatataa ccgccttccc
     1081 gcaagcctcc gtctttttcc gcgatgtctt gcttaccatt ccattttgct tcttttctgc
     1141 agtatttatt caggtactta acccaatgaa tattgcctat cgtaaacggg aagcggataa
     1201 agtactggca acccggctcg cgctgcgtac ccaccgaatt agttatatca cgctcatcgc
     1261 ggtgatcctg ttttttgcct tttcgtttac cttctcaatt agccacgaag aagccgtttc
     1321 tgcctttgaa caaaatatct cagcactggc gctggccgcg caggtgatcc ctgggcatat
     1381 cattcatatc acctctacgg tgcttaatat ctttgccgta ctgaccgcat tctttggcat
     1441 ttatctcggt ttccacgagg ccattaaagg cattattctc aatctgttaa gccgaattat
     1501 tgataccaag aaaattaact cacgcgtgct gactctggcg atctgcgctt ttatcgtcat
     1561 tacgttgacg atttgggttt cgtttcgtgt atcggtgctg gtgttctttc agttgggaag
     1621 cccgttatat ggtattgtgt cgtgcctcat tccgtttttc ctgatctata aagtcgcaca
     1681 actggaaaaa cttcgcggat ttaaagcctg gctgattctg ctgtacggca ttttgctatg
     1741 cttgtcgcca ctgttgaagc tgattgagta aaccggagcg catggccccg gttttgtgag
     1801 ttaacgctgc ggattttcat cctgatcaac agcaaaacaa gctaccagtt gaccgccgta
     1861 gtcttttagc tgcggctgca actgggtgca ggggccgaag cgccgacgac agcgggcgtt
     1921 gaaggcgcaa cccggcggtg gattcagtgg gcttggtagt tcaccgctga gcttgatgcg
     1981 ctcgcggcga tcgtccgggt tcaggcgcgg cgtcgcggaa agtagcgcct gagtgtacgg
     2041 atggcgcggg ttattgaaga tttggtcttt cgttcccttc tccacgcagc ggcccaggta
     2101 catcaccatc acttcatcag caatgtgctc caccaccgac aggtcgtggg agataaagac
     2161 ataagacagc cccaactcct gctgcaaatc catcatcaga ttcagcacct gcgcgcgcac
     2221 tgaaacatcc agcgcggaaa ccggttcatc ggcaatcacc acatccgggt cgagcatcag
     2281 accacgggcg atggcgatac gctgacgctg accgccggag aacatatgcg gatagcggtc
     2341 atagtgctcg gttttcaggc cgactttcgc catcatcgac agggcttttt cccgacgctg
     2401 ttctttgctt aagctggtgt tgatcagcag cggctcttca agaatttgcc cgactttttt
     2461 acgcggattc agcgaaccgt aagggttctg gaagacgatc tggattttct gccgacgcag
     2521 cttctgcgcc tgcggatcgt gcttaagcag atcctgcccc tggtaataca gctcgccacc
     2581 ggtgggcgtt tcaatcatcg tcagcaaccg accgagggtc gatttaccgc agccagattc
     2641 gcccactact gccagcgttt tgccacgttc aaggttaaac gaaacgccat ccagcgcttt
     2701 aaccagacgt tccggcgcga acatgccttt cttcaccgga taatgttttt tcaggtcgat
     2761 agcctgcaac agcggttgtt gcagggtggc ctcttgcgta ctcatagtgt cggcctcccg
     2821 gcatcatcaa gtgggtaatg gcatttggac tggcgcccgt cagcgagcat attcagcgcc
     2881 ggttcttcag cgcgacatct gtcagtggca taggggcagc gcgggttaag caggcagccg
     2941 ttcgggcggt cgtacttgcc gggaacgaca cctggcaacg acgccagacg ttctttgtcc
     3001 tgagcaaatt ctggcagcgc acgcagcaat gcctgagtat acgggtgacg cggcgcatgg
     3061 aagatggcgt gcgcatcgcc cgtttccacc acctggcctg catacatcac aatgatttta
     3121 tgcgcagctt ccgccaccag cgccagatca tgggtaatta acaccagcgc catgttctct
     3181 ttctgctgta gctccagcaa cagttcgatg atttgcgcct gaatggtcac gtccagcgca
     3241 gtggtcggct catcggcaat cagcagtttt ggccgacagg caatcgccat ggcgatcatc
     3301 acgcgctggc tcatgccgcc ggaaagctga tgcgggtaaa catccagacg cgatgccgga
     3361 tcgggaatac cgacctgatt cagcaggtca atcgcccgct gacggcgagt gctcttatta
     3421 ccgccctgat gcaccttaat cgcttccatg atctggaaac ccacggtgta gcacgggtta
     3481 aggctggtca tcgggtcctg gaagatcatc gccacttcgg cacccaccag gttgcggcgc
     3541 tctttttctg atatacgctg caaatcctga ccgttaaaca ccagcttttc cgccattacg
     3601 cggcccggat aatcaatcag ccccataatc gccagtgaac tgaccgactt accggagcca
     3661 gactcaccca caatcccgac cacttcgccc tgttttacgc tgtagctgat gcggtctacg
     3721 gcgcggaacg gtgcgctttc gtcgccgaaa tgcaccgata atttatctac atttaataac
     3781 gccatctcga acctcttact gcttcagttt gggatcgagc gcgtcacgca gaccgtcacc
     3841 catcaggtta aatgccagca ccgtcagcag gatcgccaga cccgggaagg tcacgaccca
     3901 ccaggcgctt tgcgcgaact gcaacacgtc ggagagcatg gtgccccact ctggtgttgg
     3961 cggctgcgct cccatgccga ggaagccaag ggcggccata tcgagaatgg cgttagagaa
     4021 accgagcgac gcctgaacaa tcagcggcgc caggcagttc gggaagatgt taataaacat
     4081 ctgacgcatc gccccggcac ctgccacgcg agacgcggta acgtaatcgc gattcacctc
     4141 caccagcacg gcggcgcggg ttaagcgcac atagtgcggc aaggcaacga aggtcagtgc
     4201 cagcgcggcg ttaccaatcg acgggccgaa aatcgccacc agcaccagtg ccaacagcag
     4261 acttggcagc gccagcatga tatcgaccac gcgcatgatg atgttatcga ccaggccgcc
     4321 gaagtaaccg gcgatcaggc cgagaataac gcccataatc agcgataaca ccaccaccag
     4381 acagccaacc agcagcgaca agcgcgcgcc gtacatcagc cgcgacagca catcacggcc
     4441 tacgtcatcg gtgcccagca agtgcgccat gctgccgcct tcctgccagg ctggcggggc
     4501 gagcagcgca tcgcggaact gatccgccgg gttataaggc gcaatccagt tggcaaagat
     4561 cgtgataaac agcacgatga cgatgtaaac cagcccgaca accgcgcctt tgttgcgttt
     4621 aaaatagtgc cagaactcct gtaacggggt catcggcacc ggtgcgctaa tcactttatt
     4681 ttcagtaacc tgtgacatga tgtcccctta cttcttatga cgaatacgcg ggttcaccac
     4741 gccgtacagc agatcgacca gcaggttgac gaggataatc atcgtcgcca ccagcaatac
     4801 gccgccctgc actaccggat agtcgcggcg ttgcagtgcg tcaatcaacc agcgtcccag
     4861 accgggccac gagaagatgg tttcggtcag aatcgccccc gccagcaatg ttcccacctg
     4921 caggccgata acggtcacta ccggcagcat cgcgttacgc agcgcatgga cgataatcac
     4981 ccgcatgcgg gtcaaccctt tggcgcgcgc ggtgcggatg taatcttcac ccagcacttc
     5041 cagcatcgaa gagcgcgtca tacgaacgat aaccgccagc gggatagtac ccagcacaat
     5101 agccggtaag atcatatggg caacggcgtc gataaagttg ccatcttcac cccagatggc
     5161 ggtgtcgatc agcataaaac cggttaacgg attggagtca tcgaggaaca ccatatcgct
     5221 cacgcgaccg gagacgggcg tcaggttcca gtgcaccgaa accagcatga tcagcatcat
     5281 gccccaccag aagatcggca tcgagtagcc ggtcagcgcc aggccaactg cagtgtgatc
     5341 gaaaatggaa ccgcgtttaa ccgcagccag cacaccaacc ggaataccga ctgccgtagc
     5401 aaaaatcatc gcgcagacgc caagttccag cgtggcctga aagcgcggca cgaactcttc
     5461 ccatactgga attcggcttt tcattgaaat gcctagatcg ccgtgcatta cgccccaaat
     5521 gtaatggaga tactgctgcc acatcggttt atctaagccg agttcagcca gcagctgcgc
     5581 gtgacgctca ggggagatcc cgcgttcgcc tgccatgatc atcaccggat cgcccgggat
     5641 catgtggaca aaggcaaatg tgagaagggt aataccgata aacgtgggga tgacgagtcc
     5701 caaacgtcgg agaataaact gcaacataac ccggattctc tatagtgacc cgcagcctgg
     5761 aactgcgtgt ctgtattgct cacaaatcaa ttcccgccct tatcggacag ggaatgaagt
     5821 attgctgccg gatggcgctg cacgcagagg cacatcgcca ccgggtatac agattgttga
     5881 caaagtgcgc tttgtttatg ccggatgcgg cgttaacgcc ttatccggcc tacaaaatcg
     5941 tgcaaattca atacattgca gaattttcgt aggcctgata agcggagcac atcaggcagt
     6001 tttgcctttg ccatcagtct tgtatggctt ttaattattc gatagagacg ttttcgaagt
     6061 gatgtttgcc taatggatca accacatagc ctttgacttc tttgcgtacc ggttcaaaca
     6121 cggtggagtg agcgatgatc agtgccggag tctgatcgtg catcaccacc tgcgcttgct
     6181 tgtacagttc aacgcgtttg ttatggtcgt cggtagcacg cgccggttga atcagatctt
     6241 caaacggttt gtagcaccat tttgagtagt tagaaccttg ttcagaggcg gcgcagctga
     6301 acagggtggc gaagaagtta tccgggtccc cgttatcgcc agtccagccc atcattaccg
     6361 tctgatgttc gccatctttc gcacgcttga ggtactcacc ccattcgtag gtgacgattt
     6421 tggcctgcac gcccactttc gcccagtctg cctgaatcat ctccgccatg cggcgagcgt
     6481 tcgggttata cggacgttgt accggcatcg cccacaggtc gatggagaaa cctttttcca
     6541 gacccgcttc tttcagcaag gctttcgctt tttcaggatc gtaggtgtaa tcctgaacgt
     6601 cgtcgttata gccccacatg gttggcggga tcaggttttt cgctgataca cccgcgccct
     6661 gataaaccgc tttgatgatc gcgtctttgt tcaccgcgta ggtcagtgcc tggcgaactt
     6721 tcacgtcatc cagcggtttt ttctgcacgt tataggagag gtagccgacg ttcagccccg
     6781 gcatttccat caggttgatg gatttatcct gcttcatgcg agcgatgtct gccgggttcg
     6841 ggtacggcat cacctggcat tcattcttct gcaatttcgc gtaacgcacg gaagcatcag
     6901 gggtaataga gaaaaccagc gtatcgatct gcggtttggt gccccagtag ccatcaaacg
     6961 ctttgtagcg gatacgggaa tctttttggt actgctgtaa ctggaacgga ccggttccga
     7021 ttgggttgag gtccagtttt tccggtgtac cggctttcat catcgcatca gcatattctt
     7081 ttgacagaat agaggcgaag tccattgcca gatcagcgag gaacggcgct tccgggcgag
     7141 tcagtacgaa ctgaactgtg ttgtcgtcca cctttttcac ttcactgatc agctccggca
     7201 agcccatgcc ttcgaagtat tcgtagctgc cgccagagac tttatggtac gggttttgcg
     7261 cgtttttctg acggtcgaac gagaacacca cgtcatcggc gttcagttca cgcgtcggtt
     7321 tgaattcttt attgtcgtgc cacttcacac ctttacgcag atggaaggta taggttttac
     7381 cgtcttcgct gacttcccac ttttcagcga ggcccgggat cacttcagtg gtgccgattt
     7441 taaattcaac caggcggtta taaagcggta cggaagaggc gtcataggtg gtgccggagg
     7501 taaacagctg cgggttaaac ccttccggag atccttctga gcaataaacc agagttttag
     7561 cctgaacact tgctgcgacg gtcatagcca ccaggctgag accaagcttc agcatccctg
     7621 actttttcaa ggaaatacgc attattctgc tccaattgtg atgtttgttg ttttaaccct
     7681 ttgcagtggg ttgtcgctgc ctgacctttt ttgttttttg cccggtcggg tcaacgttat
     7741 gaggtgggga tgccgtacta attaacatca gtgtaacagt gcggatcctc cataaaatgc
     7801 ccctcgtgac ctacaatctg tcaacagaat gtgaaaacgt caatacagcc aaccgggatt
     7861 tacaccaacg gtgagaatcc acaaacaaag attaaaaaaa cttcaaacag ctatttgcag
     7921 cagagaaatt tgtgctactc caacataacc agaacaatca gcttaatatt tagcaacatt
     7981 tggtgattaa gttttatgca tttaatgaaa aaaatctgag gaaaaggtgg atatctgagc
     8041 gattaatacc atgaacgcta aaacgcacag cggaaaatgc cagcgccagc cataagtaac
     8101 gcaaaaaaag atttaagcaa atataaaaaa agacaatggt ttctgtcaca aatccgttaa
     8161 tggatagtga gatatggggc gcaaagttgg caggaaaatg tgtggtgtgt tggtcgaata
     8221 aggcggtcgc gccgcatccg acaataaatg cggtgccgtt gcctgatgcg acgctggcgc
     8281 gtcttatcag gcctacgtcg agtcgaacct ccctgccccc ggaggttctc atcctcatga
     8341 gctacaaatg caaaaaagcc tgctcgttga gcaggctttt cgaa
//
LOCUS       DAZPAC010000176         8370 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZPAC010000176 DAZPAC010000000
VERSION     DAZPAC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8370
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.176"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(121..197)
                     /locus_tag="ACHUPM_003335"
     tRNA            complement(121..197)
                     /locus_tag="ACHUPM_003335"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(161..163),aa:Asp,seq:gtc)
     gene            complement(330..1061)
                     /gene="dnaQ"
                     /locus_tag="ACHUPM_003336"
     CDS             complement(330..1061)
                     /gene="dnaQ"
                     /locus_tag="ACHUPM_003336"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR0871027.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1126..1593
                     /gene="rnhA"
                     /locus_tag="ACHUPM_003337"
     CDS             1126..1593
                     /gene="rnhA"
                     /locus_tag="ACHUPM_003337"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR0871028.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1590..2312)
                     /locus_tag="ACHUPM_003338"
     CDS             complement(1590..2312)
                     /locus_tag="ACHUPM_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0871029.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2346..3101
                     /gene="gloB"
                     /locus_tag="ACHUPM_003339"
     CDS             2346..3101
                     /gene="gloB"
                     /locus_tag="ACHUPM_003339"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR0871030.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3173..4531
                     /gene="mltD"
                     /locus_tag="ACHUPM_003340"
     CDS             3173..4531
                     /gene="mltD"
                     /locus_tag="ACHUPM_003340"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR0871031.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4580..5203)
                     /locus_tag="ACHUPM_003341"
     CDS             complement(4580..5203)
                     /locus_tag="ACHUPM_003341"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0871032.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(5207..6007)
                     /locus_tag="ACHUPM_003342"
     CDS             complement(5207..6007)
                     /locus_tag="ACHUPM_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0871033.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6248..7162
                     /gene="yafC"
                     /locus_tag="ACHUPM_003343"
     CDS             6248..7162
                     /gene="yafC"
                     /locus_tag="ACHUPM_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR0871034.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7159..7962)
                     /gene="dkgB"
                     /locus_tag="ACHUPM_003344"
     CDS             complement(7159..7962)
                     /gene="dkgB"
                     /locus_tag="ACHUPM_003344"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR0871035.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8125..8201)
                     /locus_tag="ACHUPM_003345"
     tRNA            complement(8125..8201)
                     /locus_tag="ACHUPM_003345"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8165..8167),aa:Asp,seq:gtc)
BASE COUNT         2235 a         2016 c         2024 g         2095 t
ORIGIN      
        1 tgattaacac atttacagca taacaaaacc atagaccgaa gaaatagcgg gatttaaatg
       61 caaaaaaacc gcaaaattaa aaatcttgcg gttctctgaa ctcattttca tgagtgaatt
      121 tggcggaacg gacgggactc gaacccgcgc ccccctgcgt gacaggcagg tattctaacc
      181 gactgaacta ccgctccgcg ttgtgttccg ttgggaacga ggcgaatatt acggattgcc
      241 tcacccttcg tcaacggttt ttctcatctt ttgaatcgtt tgctgcaaaa atcgcccaag
      301 ccgctatttt tagcgccttt tacaggtatt tatgcccgcc agaggcagct tccgcccttc
      361 ttctccacca gatcaagacg ggcttcatga gctgcaagct cttcatctgt cgcaaaaaca
      421 acgcgtaact tacttgcctg acgtacaatg cgctgaattg ttgcttcacc ttgttgctgc
      481 tgtgtctctc cttccatcgc aaaagccatc gacgtttgac caccggtcat cgccagataa
      541 acttccgcaa ggatctgtgc atcgagtaat gccccgtgta gcgttcgttt actgttatct
      601 atttcgtagc gggcacataa cgcatcgagg ctgttgcgct taccaggaaa cattttcctc
      661 gccaccgcaa ggctatcggt gaccttacag aaggtattgg tcttcggaat atcgcgctta
      721 agcaacgaaa actcgtagtc cataaagccg atatcgaacg ctgcgttatg gatcaccaac
      781 tccgcgccgc ggatatagtc catgaactca tcggctactt cggcaaacgt gggcttatcg
      841 agcaaaaatt catcggcaat accatgtacg ccaaaggctt ccggatccac cagccgatcg
      901 ggtttgagat aaacatggaa gttattgccc gtcaggcgac ggttcaccac ttcaacggca
      961 ccaatctcaa tgatcttgtg gccttcatag tgcgcaccaa tctggttcat accggtggtt
     1021 tcggtatcga gaacgatctg gcgtgtaatt gcagtgctca tagcggtcat ttatgtcaga
     1081 cttatcgttt tacagttcga ttcaattaca ggaagtctac cagagatgct taaacaggta
     1141 gaaattttca ccgatggttc gtgtctgggc aatccaggac ctgggggtta cggcgctatt
     1201 ttacgctatc gcggacgcga gaaaaccttt agcgcaggct acacccgcac caccaacaac
     1261 cgtatggagt tgatggccgc tattgtcgcg ctggaggcgt taaaagaaca ttgcgaagtc
     1321 attttgagta ccgacagcca gtatgtccgc cagggtatca cccagtggat ccataactgg
     1381 aaaaaacgtg gctggaaaac cgcagacaaa aaaccagtaa aaaatgtcga tctctggcaa
     1441 cgtcttgatg ctgccctagg gcaacatcaa atcaaatggg aatgggttaa aggccatgcc
     1501 ggacacccgg aaaacgaacg ctgtgatgaa ctggctcgtg ctgcggcaat gaatcccaca
     1561 ctggaagata cgggctacca agtcgaagtt taagcttgtg gtttacgaca ttgccgggtg
     1621 gctccaaccg cctggcgaat tcgtggcttg tttttactct gtttcatcgg gtttagcgtt
     1681 aaaggaatag tccgtttccg ggcaacaata agttgtaagc aaccaagcgc aggaatatgc
     1741 gcattcaata gttttcctcc gtgtttgttc cacgggagaa cgtggaaacg gctggcgtgc
     1801 agcacttcaa aattcaacaa agagagccag tccagttgcc gcatcagagt aaacatccgg
     1861 ctgttatagg gcgaggtttt tcgcaatacc ggcacaagtt tgcgtaatcc cattaaacta
     1921 atgggattga agccactaat caccagccag ccatcatcaa tcaatacccg atcggtttca
     1981 cgcaataaac gatgcggatc ggtgcaccac ggcaatgtat gtgccagtag acaaacatca
     2041 acggatttat cggcaaaagg aagatgaagt ggatccgcct gcacctggac gggcattcct
     2101 tgcgcagaaa cattcacttg atgagaaacc gcgcacgctt cgcaattgat ttctgcgctt
     2161 aaattgccaa tcttaagcag atgaaaacca tacattttag tgaaccacgg gttgagctgg
     2221 cgctccagcg ccttgcgata aagctctccc cagggcaaat cgccccagcg atcaggagcc
     2281 acgacagttt gagggactct tgccggtttc atcacaacct tccgtttcac actaagaggt
     2341 aatctatgaa tcttaacagt attcccgcct ttgatgacaa ttacatctgg gttttgaatg
     2401 atgaagcagg tcgctgcctg attgtcgatc ccggagacgc agagccagta ttaaacgcca
     2461 ttgccgccaa taactggcaa ccggaggcca tatttctcac ccaccatcat cacgatcacg
     2521 ttggcggcgt aaaagaactg gtggaaaagt ttccgcaaat tgtggtgtat ggtccacaag
     2581 agacacaaga taagggaaca acacaggtag tcaaagatgg cgaaactgcc ttcgttttgg
     2641 ggcatgaatt tagtgtaatt gccacaccgg gtcacacttt aggacatatc tgttacttca
     2701 gtaaacctta tctattttgc ggcgacacgc tgttttctgg tgggtgtggt aggttgtttg
     2761 aagggacagc atcacaaatg tatcaatcac ttaataagtt aagtgcgtta cctgacgata
     2821 cattggtatg ttgtgctcat gaatatacgt tatcaaatat gaagtttgct ttgagtattc
     2881 ttccgcacga tttgtccata aatgattatt atcgtaaagt taaggagtta cgggcaaaaa
     2941 atcaaataac actacccgta attctgaaaa atgagcggca aattaatgtt tttttaagaa
     3001 cggaagatat tgatttaatt aatgtaatta atgaagaaac attattgcaa caacctgaag
     3061 agcgttttgc atggttaagg tcaaagaaag ataggttctg ataaaacttt cttgtcatcg
     3121 gctccgttcg ccgttatgat cggtcgtctt ttaagcaact attgacacac acatgaaggc
     3181 aaaagcgata ttactcgcct ctgtcctgct cgtgggttgc cagagtaccg gcaacgttca
     3241 acagcacgca cagagccttt ctgcagctgg tcaaggggaa gcagcaaagt ttacaagtca
     3301 ggcacgatgg atggacgatg ggacgtctat cgcgccagat ggtgacttgt gggctttcat
     3361 tggcgacgag ctaaagatgg gaattccgga aaatgaccgg attcgcgaac agaaacagaa
     3421 atatttacgc aataagagct atctccacga tgtaacttta cgggcagagc cgtatatgta
     3481 ctggatagca gggcaagtta aaaaacgtaa catgcctatg gaactggtac tactacccat
     3541 agtggagagc gcttttgatc ctcacgcaac gtctggcgcc aatgccgcag gcatctggca
     3601 gatcattccg agcacggggc gcaattatgg tttgaaacag acccgcaatt atgacgcgcg
     3661 tcgcgatgtt gttgcttcaa caactgccgc gctgaatatg atgcagcgtc tgaacaaaat
     3721 gtttgacggc gactggcttc tgaccgtagc ggcttataac agcggcgaag gtcgggtcat
     3781 gaaggcaatt aaaacgaaca aagcgcgtgg gaaatccacg gacttctggt cgttaccgtt
     3841 gccgcaggaa acgaagcagt acgtgcctaa aatgctggca ttgagtgata ttctcaaaaa
     3901 cagcaagcgt tatggcgtac gtctgccaac gaccgatgaa agccgtgctc tggcgcgtgt
     3961 gcacctgagt agcccggttg aaatggcgaa ggttgcagat atggcgggga tttccgtcag
     4021 caagctgaag acattcaacg ctggcgtgaa aggctccacg ctgggcgcaa gtgggccgca
     4081 gtacgtgatg gtgccaaaga agcatgcaga tcaactgcgt gaatctctcg cttcaggcga
     4141 aattgctgct gtacagtcga cgctggttgc cgacaatacg ccgcttaaca gccgtgttta
     4201 caccgtacgc tctggcgaca cgctttcaag catcgcttca cgtctcggcg taagcaccaa
     4261 agatttgcag cagtggaaca aactgcgcgg atctaagctg aagccaggcc aaagtttgac
     4321 gattggtgca ggcagtagcg cacagcggtt ggcaaacaac agcgatagca ttacgtatcg
     4381 tgtgcgcaaa ggcgattcgc tttcaagcat tgctaaacgc cacggcgtga acatcaaaga
     4441 tgtgatgcgc tggaacagcg acactgcgaa tctgcaacca ggcgataagc tgacgttgtt
     4501 tgtgaaaaac aacaacatgc cagattcctg acaaaccaga taataaaaaa ggcaccgatt
     4561 cccccggtgc cttttttatt tatgccgctt tatgtgcttc taccatgatg atatcactgg
     4621 tgaaagagcc atcagtctgc aaggaaaaat acgttctcac ctctgtcgat gcgctctgtt
     4681 ggtaaatgcg aatagcgtct actaacgctt ctggcgtacg cattctcacg acccatgaag
     4741 aaaattccag cggtaactta tctgtaatta aattatcaac tatcagattg gcttcattga
     4801 ttaacgtcaa ccacgcaccg ctggcgtagt ttcgtacgtg agaggtatcg cgtaatgctt
     4861 ctaccgcctg taaccagatg tcgcgcactg ggtgacccgg agacattaca tccatcacaa
     4921 tcagcctacc gccaggtttc aatatcctat tcacttctcg cagtgctgca ccaacatcat
     4981 gccagtgatg ggcagaataa cggctgataa caatatcaaa tgcgttatcg gcaaatggca
     5041 gacttgcggc atatccctgg cgggtggtga tatttttcag ttgccgggct tcggcagctt
     5101 gtgccacgac atccagcatg tgggcagata agtcatacgc caccaccgcg ctcacgtttt
     5161 gcgcagcgcc aaacgttggt ctgccctgcg gcagacctga cataccttat ttatcaggct
     5221 tgccgggact gaattcaacg agtagcggat tgtgatcgga agcgcgcgta accagtacag
     5281 aagcttcact gacgttcaga ccacggtaga aaacaaaatc gagcgggcga ccaaacgccc
     5341 gacggcgctg atcatcggta aaacgcacct ggcgcagcga catttcccgc gcaaagcgat
     5401 ataacgcgtt catccttcta cggctccagg cattgaaatc tcccgccata atgaccgggc
     5461 cgctgtggtg agctatctga tcaccaatag gaagtaactg cttactatag acatccacgc
     5521 ccagactgaa gttgacggcg tgtatattaa ccaccatcaa caggcgggtg tcaggtaatg
     5581 gatagaccgt caccagtgcc gacttcgcca gacgcaaaat gggttctcgt tcacgtaacg
     5641 ggcagcaata cactggatgt gccgccgaaa gggtcattac gccagaagga tgttgtggca
     5701 gcacgaaagc gggtacctga tcggcggcaa gatagttagc ggtcgcaaac tgtaccaact
     5761 ctggcgttgt ttgcgcttcc tgcaataaca ccagatgtgc gtctttgccg taattcttta
     5821 ataccgacaa ccattcagcg cgttgctgtt tgtatatgtt ccacaccagg atccgaatac
     5881 gctcttcggt acttaacggt tctccgggtg gtaatgcctg gccgatgctc gcaaaagacc
     5941 ccggcggtaa gatcctctcc gcaggttgtc cggcaacata gcgcatggca taggtgtttt
     6001 ttcgcacttt tgactttgaa acctctatta cgtcaaccct tccgcactcg ggagggttct
     6061 actgttatag ggattttagc tcactctttc aacgagcaat tgtatattgt tatgtaagca
     6121 agtgcttaca ccggtcagaa atttgctcat tattgagaac attaaatcgg atgacatatc
     6181 acgcatcctg atggtttaat gacaaataaa ttcaaaatat ctcatctatt ttgcaacagg
     6241 agcaaaaatg aaagccacgt cggaagaact cgccattttt gtttcggtcg tcgaaagcgg
     6301 cagctttagc cgggcagcgg aacaattagg gcaagcaaac tcagcggtaa gccgggcggt
     6361 gaaaaagctg gaaatgaaac ttggcgttag cctgcttaat cggaccacgc ggcaacttag
     6421 cctgacggaa gaaggcgagc gttattttcg tcgcgtacag tcaattttgc aggagatggc
     6481 agcggcagaa tcagaaatta tggagacgcg taatacaccg cgtggactgt tacggatcga
     6541 tgccgcaact ccagtggtgc tgcactttct gatgccgtta attaagcctt tccgtgaacg
     6601 ctatccggaa gtcactttat cgctagtctc ctccgaaacg attattaatt tgatcgaaag
     6661 aaaagtggat gtcgcgatac gcgctggtac gttaacggac tccagcttac gtgccaggcc
     6721 gttatttaac agttatcgaa aaattatcgc ctcccccgat tatatttccc gctacgggaa
     6781 gccagaaacg atcgacgatt taaagcaaca tgtttgcctg ggattcactg aacccgcttc
     6841 cctcaatacc tggccgatag cctgtagtga tggacaatta catgaggtga agtacggttt
     6901 gtcatccaat agtggggaaa cactgaaaca gctttgcctg agtggcaacg ggattgcgtg
     6961 tttgtccgac tacatgatcg acaaagaaat cgctcgcgga gaattggtgg agttaatggc
     7021 agataaagtg ttgccagtgg aaatgccatt cagtgccgtc tattacagcg accgtgcggt
     7081 aagtacgcgc atccgggctt ttatcgattt ccttagcgag catgtaaaaa cagctcccgg
     7141 aggagctgtc agagaggctt aatcccattc aggagccaga ccttccgggc taaccaggcg
     7201 gtcgttgcaa tccagtgcgg cgatcgcttt tttatcttcg gcatcaagct gtaaattttg
     7261 tgccttaaga ttactttcca ggtttttacg tttagtagaa gaaggaatta ctgagtaacc
     7321 ttcccccata gcccacgcca gaatcacttg tgccggagtc gcattgtgtt tagctgcaat
     7381 acgagcaata acctcatctt tcagtgcctt accataagcc agcgtcatat aggaagtgat
     7441 atgaataccg tgctgtttag cccaggcaac cactttacgg ttttgcagat aaggagagag
     7501 ttcaatctgg ttggtagcga tgttttcagc gccaacagca gcaatcgctt tttccatcaa
     7561 cgggatcgtg aagttggaaa taccgatctc acgtgtcaga ccttgttttt tggcctccag
     7621 cagcgcctgc ataaactctt caacagagac ttcatcgttt ggtgacggcc agtggattag
     7681 cgtcagatca acataatcgg tacgcaattt ttgcaggctc tcttttagac tcgggatcaa
     7741 tttgtctttg ctgagatttt caatccagat tttagtggtg atgtagagtt catgacgtgg
     7801 cacgccactt tctgcaatcg cctgacctac tgcggcttcg ttatcataga tttgtgcagt
     7861 atcaattgcg cgataaccaa gttcaagcgc cgttttcaca gatgaaataa caacgtcgtc
     7921 tttgagacgg aaagtaccta aaccaaatgc agggatagcc ataatatgcc tcttttaatg
     7981 ctattttcgt taactgaggt gattatgggc ttcagactgt tgcggaaaaa gagcataaaa
     8041 tgcagaggat ttttgcgatt ctggcaataa tagatataca gacgaaaaaa aacctcgagc
     8101 gttaactcga ggcttcttaa taagtggcgg aacggacggg actcgaaccc gcgaccccct
     8161 gcgtgacagg caggtattct aaccgactga actaccgctc caccgaattc ttttacaacc
     8221 accggtttta tgaccggctt actgcttaat ttgatgcctg gcagtttatg aatcacttcg
     8281 ggctgttgct tcgcaacgtt caaaagcttt cgcttttgtc ctactctctt atcgagtagt
     8341 gaactgcctc aaaggtcagt gccattcttc
//
LOCUS       DAZPAC010000177         8331 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZPAC010000177 DAZPAC010000000
VERSION     DAZPAC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.177"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..188
                     /locus_tag="ACHUPM_003346"
     CDS             <1..188
                     /locus_tag="ACHUPM_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871036.1"
                     /translation="MLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALF
                     PIFTASWRATRIKNIGI"
     gene            411..908
                     /gene="ubiC"
                     /locus_tag="ACHUPM_003347"
     CDS             411..908
                     /gene="ubiC"
                     /locus_tag="ACHUPM_003347"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR0871037.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            921..1793
                     /gene="ubiA"
                     /locus_tag="ACHUPM_003348"
     CDS             921..1793
                     /gene="ubiA"
                     /locus_tag="ACHUPM_003348"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR0871038.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHUPM_003349"
     CDS             complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHUPM_003349"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR0871039.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHUPM_003350"
     CDS             4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHUPM_003350"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR0871040.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5020..5628
                     /gene="lexA"
                     /locus_tag="ACHUPM_003351"
     CDS             5020..5628
                     /gene="lexA"
                     /locus_tag="ACHUPM_003351"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR0871041.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5701..7026
                     /gene="dinF"
                     /locus_tag="ACHUPM_003352"
     CDS             5701..7026
                     /gene="dinF"
                     /locus_tag="ACHUPM_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR0871042.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7142..7351
                     /locus_tag="ACHUPM_003353"
     CDS             7142..7351
                     /locus_tag="ACHUPM_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR0871043.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHUPM_003354"
     CDS             complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHUPM_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR0871044.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8226..>8331
                     /gene="yjbL"
                     /locus_tag="ACHUPM_003355"
     CDS             8226..>8331
                     /gene="yjbL"
                     /locus_tag="ACHUPM_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR0871045.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRG"
BASE COUNT         1919 a         2013 c         2261 g         2138 t
ORIGIN      
        1 atatgttgaa tcctacaaaa gataccaatt ggaattccac gtgtatctat aaatccagac
       61 ataagatgtt gcctattaat cttactcagg aaacactttt tagctccaaa tctcatggta
      121 aatatgcgct tttcccaatt tttactgcga gttggcgagc tactcgtata aagaatatag
      181 gtatttaagt aaaggaaaac atcaccgttc ctggcatcat gggcggtgat gcccctacgg
      241 ttgccctcgc cagcacgggc atcggcaaag cgtaaggttc aacagcgttt taccacttca
      301 tgcgattgtt gcgtttttgt tgcgcattag atcacttaat ttgctttaca tctcccgtaa
      361 acacttttct gcgatacaat gcctttacgt tatgtaacgg agagttcggc atgtcacacc
      421 ccgcgttaac gcaactgcgt gcgctgcgct attttaaaga gatccctgcc ctggattcgc
      481 aactgctcga ctggctgttg ctggaagatt ccatgacaaa acgttttgaa cagcagggaa
      541 aaacggtgag cgtgacgatg atccgcgaag ggtttgtcga gcagaatgaa atccccgaag
      601 aactgccgct gctgccgaaa gagtctcgtt actggttacg tgaaattttg ttatgtgccg
      661 atggtgaacc gtggcttgcc ggtcgtaccg tcgttcctgt gtcaacgtta agcgggccgg
      721 agctggcgtt acaaaaattg ggtaaaacgc cgctaggacg ctatctgttc acatcatcga
      781 cattaacccg ggactttatt gagataggcc gtgatgccgg actgtggggg cgacgttccc
      841 gcctgcgatt aagcggtaaa ccgctgttgc taacagaact gtttttaccg gcgtcaccgt
      901 tgtactaaga ggaaaaaaat atggagtgga gtctgacgca gaataagctg ctggcgtttc
      961 atcgcttaat gcgtacggat aagccaattg gcgcgttact gctgctctgg ccaacattat
     1021 gggcgttgtg ggtggcgaca ccgggcgttc cccagctctg gatcctggcg gtgtttgtcg
     1081 cgggtgtctg gctgatgcgc gctgccggat gtgtggtgaa tgattatgct gaccgcaagt
     1141 ttgatggtca tgttaagcgc acggcgaacc gaccgcttcc cagcggcgcg gtaacggaga
     1201 aagaggcgcg cgcgctgttt gtagtgctgg tattgatttc atttttactg gtgctgacgc
     1261 tgaatacgat gaccattctg ttgtcgattg ccgctctggc gttggcgtgg gtgtacccgt
     1321 ttatgaagcg gtatacccat ctcccgcaag tggtgctggg cgcggcgttt ggctggtcga
     1381 ttccaatggc ttttgccgct gtgagtgagt cggtgccatt gagttgctgg ttaatgttcc
     1441 tcgccaatat tctctgggcg gtggcttacg acacgcagta tgcaatggtt gaccgtgacg
     1501 atgatgtgaa gattggtatt aaatccaccg caatcctgtt cggccaatac gataaattga
     1561 ttattggtat tttgcagatt ggcgtactgg cactgatggc gatcatcggt gagttaaatg
     1621 gcttaggctg gggatattac tggtcaattc tggtggctgg cgcgctgttt gtttatcaac
     1681 aaaaactgat tgccaaccgc gagcgtgaag cctgctttaa agcatttatg aataataact
     1741 atgttggtct ggtactgttt ttaggactgg caatgagtta ctggcatttc tgatgatgta
     1801 aaaaagccgg atgatcatcc ggctttcttc tgggttgcct gatgcgctgc gcttctcagg
     1861 cctacacaac acatcgcaat ttattgaatt tgcagattat ggaaggccgg ataaggcgtt
     1921 ttcgccgcat ccggcaattc tctctgatta cccttcgccc tgcgtcgcac tctcaatcgt
     1981 caaacgcacg tctgatgtaa tcaactccgc cagcaactga taaaccttca tcgtttctgc
     2041 cggttcggcg tcgccgctat cgctgatata cccttcatca cgcagcgtca gcaccagaga
     2101 actgaacacc gccttgtcga agaactccgg cgcgttgatg ccgtgcagca cggagagacg
     2161 ttgcgcaacg gtgcggctct ctttctccag cgtaccgcgg ttgatcgacg ggttggcact
     2221 caacaaccag aaggtgatgg cataacgttg cagcgtttcg cgcgcgcctg cggccagcag
     2281 ctgtaggggg cgagaatgcg ccgggttgat atgcaactca tcatcttgca gggtaatcag
     2341 cccctgacgt tgcatctcat ttgccagcgc atcaataacg tccggcaact cgtcgcgatc
     2401 ccagcgcagg aacagctccg ctttcagcat tgggtaaagc acattgacgt gctccatcaa
     2461 tacgtcgcgg gagatgtggc gatgctgggt gacaattgcc gccatcagcg aaggcagcac
     2521 caacatatgc gcaatgttgt tgcgatagta ggtcatcagc accgcttgct cgcgcggcag
     2581 aatgatgatg tcgccgatag tgtctttctc gacttcaaac ttgttcattt gcagcgcgtg
     2641 atcgataagc tcgctggcgc tggctgaagg aacggtagag tccgtggagt aaggtacgtt
     2701 gcgcatcaga tccaggtagc agttgagttg ctcggttaac tgctcgcggg tgagtgagcg
     2761 ctgacgtgat gccagaagcg cagtacaaca caggttcatg gcgtttgccg cgcctgcgtt
     2821 gttaatgcgt accatcagat cggcagcaat attattgacc gtcggcgtta accaggccgg
     2881 acgcaccgct tcgatgggat cgatagattc acgccagtct ggtacgtgct ggttaaggta
     2941 ggtcatcaac ggcattggtt caccgaagtt gacgtaaccc tgaccgagat tacgcagctt
     3001 gcttaaaccg cgcagcatct gcggcaggct ctctttctct ttcgtcgcgc cgcgcagttc
     3061 tttggcgtaa gtacccactt ccatgacgtg ctcataaccg atatagatcg gaatcagcgt
     3121 aatcggacgc gtgccgccac gcagcatcgc ctgaatggtc atcgacagcg taccggtttt
     3181 cggatccagc aaacgccccg tgcgggaacg accgccttcc acgaagtact cgacggaata
     3241 accacggctg aacagttcgc cgagatactc acggaaaacg gtggaataaa gtttattgcc
     3301 tttaaaagta cggcgaataa agaacgcccc cagacggcgg aaaatcggcc cggccggcca
     3361 gaagttcagg ttgatcccgg cggcgatatg cggcggcacc agcccctggt ggtacagcac
     3421 gtaagaaagt agcaggtagt ccatgtgact gcggtggcaa ggcacataca ccagctcatg
     3481 accgtcgtgg gccagctggc gaacgcgctc ggcgttatgg acgttgatgc cctgataaag
     3541 tcggttccag gtgaagccca gaatgcggtc agtcaggcgg atcatctcgt aagagaaatt
     3601 cgccgcaatc tcttccatca gcgcaatcgc gttctgctgc gctttttcat gggagatttt
     3661 tttgctgcgc gcttcatctt ctaccgcttt ggcaatggcg cgggaggcga gcagcttatt
     3721 aaacagatcc tgacgagcag gcagacgtgg gcctacggca gccagacgtt gacgggcaaa
     3781 gtgcatacgc gccacgcgcg ccagtttctg tgcgatagtt ttatccgtgc cgtgttcatc
     3841 cgccatacgg cgcagcgaaa ctgacggcga gaaacgcaca aaactgtcgc gaccgagcca
     3901 cagtacggcg aaaaatttct gtacgccgtt aagcatacgc agcggcgggt ttacttcgcc
     3961 tttttcacgc cccggcgcgc gaccaaacat caccgatact ggcaccatct gcacatccag
     4021 atttgggttg ctacggtgca aatcgagata gtcgtggaac agcttaatag actcttcttt
     4081 cggcgtgtaa taggtgaata cacgcggccc gccgtgaatg aacacatagc gcggcagtag
     4141 cgtgccgtcg atttctagcg gctctaacgg gtcaggcaag tcatgagcca gacactgggc
     4201 gcgtaacgtc agcaaatctg ctttcgagtt gtacggtaaa acgtacataa ttggacgaga
     4261 ggtatccagc cccagttccg gggcaggatc tgccggaata gacttgcttt ttaccaggat
     4321 gcttaatggt aaattcagta atttgtagta aattcgtggc cagccggaca taaacgatgt
     4381 aaagcctctg gttaataatg caaatgcgcg gcaaggatag cagaaagtca tgggaaattc
     4441 tgtggtatcc gctcatgttt cgcgcggcgt tacgcaaacc cgaatcatcg gatttaacgg
     4501 tacactgata ttgacgctca taatgtaaaa aggttctttc aatggccaat aataccactg
     4561 gattcacccg aattatcaaa gctgctggct attcctggaa aggtttacgc gctgcatgga
     4621 tcaacgaagc ggcattccgt caggaaggcg tagcggtatt gttggcggtg gtcatcgcct
     4681 gctggctgga tgtggacgca attacccgcg tgctgcttat cagctccgtg atgctggtga
     4741 tgattgtgga aattctcaat agcgccatcg aagcagtggt tgaccgaatt ggctctgaat
     4801 accatgagct ttccggacgc gctaaagata tgggatccgc cgcggtgctg attgcgatta
     4861 tcgtcgccgt gataacctgg tgcatcctgt tatggtcgca ttttggataa cccttccaga
     4921 tttcgataaa tctctggttt attgtgcagt ttatggttcc aaaatcgcct tttgctgtat
     4981 atactcacag cataactgta tatacaccca gggggcggaa tgaaagcgtt aacggccagg
     5041 caacaagagg tgtttgatct catccgtgat cacatcagcc agacaggtat gccgccgacg
     5101 cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa acgcggctga agaacatctg
     5161 aaggcgctgg cacgcaaagg cgttattgaa attgtttccg gcgcatcacg cgggattcgt
     5221 ctgttgcagg aagaggaaga agggttgccg ctggtaggtc gtgtggctgc cggtgaaccg
     5281 cttctggcgc agcagcatat tgaaggtcat tatcaggtcg atccttcctt gttcaagccg
     5341 aatgctgatt tcctgctgcg cgtcagcggg atgtcgatga aagatatcgg cattatggat
     5401 ggtgacttgc tggcagtgca taaaactcag gatgtacgta acggtcaggt cgttgtcgca
     5461 cgtattgatg acgaagttac cgttaagcgc ctgaaaaaac agggtaataa agtcgaactg
     5521 ttgccagaaa atagcgagtt taaaccaatt gtcgttgacc ttcgtcagca gagcttcacc
     5581 attgaagggc tggcggttgg ggttattcgc aacggcgact ggctgtaaca tatctctgag
     5641 accgcgatgc cgccaggcgt cgcggtttgt ttttcatctc tcttcatcag gcttgtctgc
     5701 atggcattcc tcacttcatc tgataaagca ctctggcatc tcgccttacc catgattttc
     5761 tccaatatca ccgttccgtt gctgggactg gtcgatacgg cggtaattgg tcatcttgat
     5821 agcccggttt atttgggcgg cgtggcggtt ggtgcaacgg cgaccagctt tctctttatg
     5881 ctgttgctgt ttttacgcat gagcaccacc gggctgactg cgcaggctta tggtgccaaa
     5941 aatcctcagg cattagcccg tgcgctggtg caaccgttgc tgttggcgtt gggggctggg
     6001 gcgttaattg cgctgctgcg tacaccgatt atcgatctgg ctctgcatat tgttggcggt
     6061 agtgaagcag tcctggaaca ggcgcggcgc tttcttgaaa tccgctggtt aagcgcaccg
     6121 gcgtcgctgg cgaatctggt attactcggt tggttactcg gcgtgcaata tgcccgtgcg
     6181 ccagtaattt tgttagtggt cggcaatatc ctcaacattg tgctggatgt ctggctagtg
     6241 atggggctgc atatgaacgt gcagggcgcg gctctggcga cggttattgc ggaatatgca
     6301 acattgctga ttggtctgct aatggtgcgt aaaatcctca aactacgcgg aatttccggc
     6361 gaaatgtcga aaactgcctg gcgaggaaac ttccgtcgct tgctggcgct taaccgcgat
     6421 atcatgctgc gttcgctgtt gttgcaactc tgtttcggcg cgatcaccgt acttggcgcg
     6481 cgactgggga gtgacattat cgctgtaaac gcggttctga tgacgctact cacctttacc
     6541 gcctatgcgc tggatggttt tgcctacgcg gttgaagcac attccggtca ggcatacggt
     6601 gcgcgcgacg gtagccagtt gctggatgtc tggcgggcag cgtgccgcca gtcggggatc
     6661 gtagcgttac tattttcggt ggtttatttg ctggcagggg aacacatcat tgcgttgctg
     6721 acgtcgttaa cccagattca gcagctggct gaccgctatc ttatctggca ggtgattttg
     6781 ccgttggttg gcgtctggtg ttatctgctg gacggcatgt ttataggcgc aacgcgtgcc
     6841 gccgaaatgc gtaacagtat ggcggtggcc gccgcaggtt ttgcgctgac gctccttacg
     6901 ctgccgtggc tgggtaatca tggtttgtgg ctggcactaa ccgtctttct ggcgttgcgc
     6961 gggctttctc tggcggctat ctggcggcgt cactggcgta acggtacctg gtttgccgca
     7021 acgtgacggt taaaaattct gaataaatta tcctaagcca aattgctgac tacacttaat
     7081 ctcacgttca gaagaaaagt gaacgtactc tcattcacaa cctaacgatg aggtcttgat
     7141 tatgaataaa gatgaagccg gcggtaactg gaaacagttt aaaggtaaag tgaaagagca
     7201 atggggcaaa ctgaccgatg atgatatgac gatcattgaa ggtaaacgtg atcaactggt
     7261 cggtaaaatc caggaacggt atggttatca gaaagatcag gcagaaaaag aggtcgtgga
     7321 ttgggaaacc cgcaatgaat atcgctggta attaatccct cctgcccgac gtgtacaagg
     7381 atgtacgccc tcttaacgcg gtttcttttt cacctgcaca gagtgatcat gctggcactg
     7441 ttcaggatga cgacacgctt ccacttccac gcatgccgca cagagcccat gtgcttcaat
     7501 cacattatgc cgcagggcaa accccatttt tgccgccagc gtatgcataa tgtcttccac
     7561 gccttctgca cactcttctt tcaccgcgcc gcagcgatcg catataaaca tggctgacgt
     7621 atgcgtgggc tggtcgaaca gatgacagag cacataactg ttggtggatt ccaccttatg
     7681 cacaaaacct tgctcaagca gaaaatccag cgcgcgataa accgttggcg gcttggcttg
     7741 cggttcagct tcgcgcagta aatcaagcag atcataagcg ctgatagcgc catcctgcag
     7801 actcatcagg cgcaacactt ccaggcgctg tggggtcagg cgcacattac gctgcgcgca
     7861 gattttttca gcctgcgcta ataactcctg cgttgtggtc ttttccatcg ggcacctcaa
     7921 agtgggggtt aagaatgtcc ttactttacc atgttccagg aaaaacaccg agaaccctta
     7981 ttgttgccgt aatgttgatt ttctgttttg taggtaaggt gttatgttgc cttgtcgtac
     8041 cattatcaac acgataataa ttaataattc atttttaaat aaattgcttc aagttaactc
     8101 gctatttatt taactgggtt taaacgcata cctgtagttc agaaattatg agcgattttt
     8161 gatagtgacc agatggcaaa agaattataa ggaggacacg gtacgacaaa aaaggtagat
     8221 gaatcatgtt gaagataata cccggtgcaa ctggatactt taataaaaca ctcaattcaa
     8281 atcaattcga taatgaagat gcaatcaaag ataaattaga taatagaggt t
//
LOCUS       DAZPAC010000178         8324 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZPAC010000178 DAZPAC010000000
VERSION     DAZPAC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8324
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.178"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(6..212)
                     /locus_tag="ACHUPM_003356"
     CDS             complement(6..212)
                     /locus_tag="ACHUPM_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR0871046.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            525..614
                     /gene="kdpF"
                     /locus_tag="ACHUPM_003357"
     CDS             525..614
                     /gene="kdpF"
                     /locus_tag="ACHUPM_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR0871047.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            614..2287
                     /gene="kdpA"
                     /locus_tag="ACHUPM_003358"
     CDS             614..2287
                     /gene="kdpA"
                     /locus_tag="ACHUPM_003358"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR0871048.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2310..4358
                     /gene="kdpB"
                     /locus_tag="ACHUPM_003359"
     CDS             2310..4358
                     /gene="kdpB"
                     /locus_tag="ACHUPM_003359"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFR0871049.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4367..4939
                     /gene="kdpC"
                     /locus_tag="ACHUPM_003360"
     CDS             4367..4939
                     /gene="kdpC"
                     /locus_tag="ACHUPM_003360"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR0871050.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4932..7616
                     /gene="kdpD"
                     /locus_tag="ACHUPM_003361"
     CDS             4932..7616
                     /gene="kdpD"
                     /locus_tag="ACHUPM_003361"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFR0871051.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7613..8290
                     /gene="kdpE"
                     /locus_tag="ACHUPM_003362"
     CDS             7613..8290
                     /gene="kdpE"
                     /locus_tag="ACHUPM_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFR0871052.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1725 a         2175 c         2430 g         1994 t
ORIGIN      
        1 agcaatcagt aaaaatcacc ggttgccttt tcggcctgat ccatccacac cggtttatcg
       61 ctggttttcg accagacgcg gtgcaggtag ctgtaaaagc gggcgcggtc tttccagaaa
      121 agcatgaaag gcagcgccag aacgcccgct gcaaccgcat atgtacggcg taaaaagata
      181 agccatgcag gatattctct gtagagttcc atacttttct cccccttttg tatctacccg
      241 gtgaatagca ccggaaaaat gaatttgttt atctgatgaa aatagtaccg ccttttgtgt
      301 aattttacta ctcatccgac cacttatttt tgcttattga tggtttattt acattcactc
      361 tgtaattaag ttacacaaaa gttaaattaa tactaaacat tagttaaatc atggcttttg
      421 ccatttttat acttttttta caccccgccc gcagattttt gcgaaatctt tgcagccaga
      481 attctactct tccggtatca cttttaggcc actggaggtg cactgtgagt gcaggcgtga
      541 tagccggcgt gttgctggtg tttttattac tgggttattt ggtttatgcc ctgatcaatg
      601 cggaggcgtt ctgatggctg cgcaagggtt cttactgatt gccacgtttt tactggtatt
      661 aatggtgctg gcgcgtcctt taggcagcgg gctggcgcgg ctgattaatg acattcctct
      721 tccaggtaca gcgggcgttg aacgtatact ctttcgttta cctggcgtct ctgaccatga
      781 gatgaactgg aagcaatatc tttgtgccat tctcggcctg aacatgctgg ggctggcggt
      841 gctgtttttt atgttgctcg gtcagcacta tctgccgctt aatccacagc agttgccggg
      901 gctgtcgtgg gatctggcgc tgaataccgc cgtcagcttt gtcacgaata ccaactggca
      961 atcttatagc ggtgaaacca cgttgagcta tttcagccag atggcaggct taacggtaca
     1021 aaactttctt tctgccgcca gcgggattgc ggtgattttt gccttcatcc gtgcgtttac
     1081 tcgccagagc atgagcacac tcgggaatgc ctgggtcgat ctgctacgca tcacgttatg
     1141 ggtgctagtc cctgtggcgt tgttgatcgc actgtttttt attcaacaag gtgcgctgca
     1201 aaactttctg ccctatcagg cagtgaatac cgttgaagga gcgcaacagc tgttacccat
     1261 gggaccagta gcttctcagg aagcgatcaa gatgctcggt actaacggcg gtggcttctt
     1321 taatgccaac tcgtcgcatc cgtttgaaaa cccaaccgca ctgaccaact tcgtgcagat
     1381 gctggcgatc ttcttgatcc caacagcgct gtgctttgcc tttggtgaag tgacgggcga
     1441 tcgccgccag gggcgcatgt tgctgtgggc gatgtcagtg atttttgtca tctgcgtagg
     1501 cgtggttatg tgggcagaag ttcagggtaa tcctcatctg ctggcgctcg gcgcagacag
     1561 cagcatcaat atggaaggta aagagagccg tttcggcgtg ctggtcagta gcctgtttgc
     1621 ggtcgtgact acggcggcgt cctgtggcgc ggtgattgcg atgcatgatt cgtttaccgc
     1681 tctcggcggc atggtgccga tgtggctaat gcaaattggt gaagtggtgt tcggcggtgt
     1741 cggttctggt ctttacggca tgatgctgtt tgtcctgctg gcggtgttta ttgccgggct
     1801 gatgattggt cgtacaccgg aatatctggg taaaaaaatc gacgtacgcg agatgaaact
     1861 gactgcactg gcgattctgg ttactccaac actggtactg atgggcgcgg cgttggcgat
     1921 gatgaccgac gccggacgta gcgccatgct taaccctggc ccgcatggtt ttagcgaagt
     1981 gctgtacgcc gtgtcgtccg ccgctaacaa caacggcagc gcctttgccg gattaagcgc
     2041 caactctccg ttctggaact gtttactggc gttctgcatg tttgtcggtc gcttcggggt
     2101 gattatcccg gtgatggcaa ttgccggttc gctggtgagt aaaaagagcc aacccgccag
     2161 ctccggcacg ctgccaacgc acggcccgct gtttgttggc ctgttaatcg gcaccgtgtt
     2221 gctggttggc gcactgacct ttatccctgc cctggcgctt ggtccggtgg cggaatatct
     2281 ctcctgatga tattgagcga gcactgaata tgagtcgtaa acaactggcg ctattcgaac
     2341 caacacttgt cgttcaggcg ctgaaagaag cggtgaaaaa attaaacccg caggcgcaat
     2401 ggcgcaatcc ggtgatgttt atcgtctgga tcggcagtct gctgaccacc tgtattagca
     2461 tcgcgatggc aagcggtgcg atgcctggca atgcgctgtt tagcgcggca attagcggct
     2521 ggctgtgggt caccgtactg ttcgctaatt tcgccgaggc gctggcagaa ggccgcagta
     2581 aagcgcaggc caacagtctt aaaggggtga aaaaaactgc ctttgcccgc aagctgcgtg
     2641 agccgaaata tggcgctgcg gcggacaaag ttcctgccga tcaactgcgt aaaggtgaca
     2701 tcgtgctggt agaagctggc gatattatcc cctgcgatgg tgaagtcatt gaaggcggcg
     2761 catcggtcga tgaaagcgcc atcaccgggg aatcggcacc ggtgatccgt gaatccggcg
     2821 gcgattttgc ctccgtcacc ggcggcacgc gtattctttc tgactggctg gtgattgagt
     2881 gtagcgttaa ccccggtgag acatttctgg atcggatgat cgcgatggtg gaaggcgcac
     2941 agcgacgcaa aacaccgaac gagattgccc tgactatcct gctgattgct ctgaccatcg
     3001 tctttttact ggcaaccgcc acgctgtggc cgttttccgc gtggggcggt aatgccgtga
     3061 gtgtaacggt actggtggcg ctgctggtct gtctgatccc aaccactatt ggcggcctgt
     3121 tgtcagcgat cggcgtcgcc gggatgagtc ggatgctggg cgcgaatgtg attgccacca
     3181 gcggacgtgc agttgaagcg gcaggtgacg ttgacgttct gctactggat aaaaccggca
     3241 ccatcacact cggtaaccgt caggcgtcgg agtttatccc cgcgcagggc gtggatgaaa
     3301 aaacgctggc tgacgccgca caactggctt cgctggctga tgaaacgccg gaaggtcgca
     3361 gtattgtgat cctcgccaag caacgtttta acctgcgcga gcgcgatgtg cagtcgctcc
     3421 atgccacctt tgtaccgttt actgcgcaaa gccggatgag cgggatcaac atcgacaacc
     3481 gcatgatccg taaaggttct gtcgatgcca ttcgccgcca tattgaggct aacggcggtc
     3541 acttccctgc cgatgttgat caaaaagtcg atcaggttgc gcgtcaggga gccacgccgc
     3601 tggtggtggt ggaaggttct cgtgtgctgg gcgttattgc gctgaaagat atcgtcaaag
     3661 gcggtattaa agagcgcttc gcccagctgc gcaaaatggg cattaaaacg gtgatgatta
     3721 ccggcgataa ccgtctgact gccgccgcga tagctgcgga agcgggtgtc gatgattttc
     3781 tcgccgaagc aacaccggag gccaagctgg cattgatccg tcagtatcag gcggaaggtc
     3841 gtttggtagc gatgaccggc gacggcacca acgatgctcc ggcgctggcg caggcagatg
     3901 tcgcggtggc gatgaactcc ggcacccagg cggcgaaaga ggcgggcaat atggtggatc
     3961 tcgactctaa cccgaccaag ttgatcgagg tggtgcacat tggcaaacag atgctgatga
     4021 cccgtggctc gctgaccacc ttcagcattg ccaacgatgt ggcgaaatac ttcgccatta
     4081 ttccggcggc gttcgcggca acatatccgc agttaaatgc gctgaacatc atgcgcctgc
     4141 attcgcccga ctccgccatt ctcagtgcgg tcattttcaa cgccttgatt atcgtctttt
     4201 tgattcctct ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct gccatgttgc
     4261 gccgtaactt atggatttac ggtctgggtg ggctgctggt gccgtttatt ggtatcaaag
     4321 tcattgattt actgctgacc gtttgcggtc tggtgtgagg tttaccatga gtggattacg
     4381 tccggcatta tcgacatttc tctttctgtt attgattact ggcggcgttt acccgctgct
     4441 gaccaccgca ctggggcaat ggtggtttcc ctggcaggcc aacggttcgt tgatccgtga
     4501 aggtgatacg gtgcgtggct cggcattaat cgggcagaat tttaccggca acggctattt
     4561 tcatggtcgc ccgtcagcaa cggctgaaat gccctataat ccacaggctt ctggcgggag
     4621 caatctggcg gtcagtaacc ctgagctgga taaactaata gccgcacgcg ttgctgcatt
     4681 acgggccgct aacccggatg ccagcgcgag cattccggtt gaactggtga cggcatcggc
     4741 aagcgggctg gacaataata tcaccccgca agcggcggcc tggcagatcc cacgcatcgc
     4801 gaaagcgcgt aatctcagcg ttgaacagct cacgcagctg atcgcaaaat acagccaaca
     4861 accgctggtg aaatatatcg gccagccggt cgtcaacatt gtcgaactca atctggcgct
     4921 ggataagctt gatgaataac gaacccttac gtcccgaccc cgatcgtctg ctggaacaaa
     4981 ctgccgcgcc gcatcggggg aagctgaaag ttttcttcgg tgcctgtgca ggcgtcggga
     5041 agacctgggc gatgctggca gaagcccagc gactgcgggc gcaagggctg gatattgtgg
     5101 ttggcgtggt agaaacccac gggcgaaaag ataccgccgc aatgctggaa gggctggctg
     5161 ttctgccgcc aaaacgcctg gcgtaccgtg ggcggcacat ctgcgagttt gacctcgatg
     5221 ccgccctcgc ccgccgtccg gcgctgatct taatggacga actggcgcac agtaatgcgc
     5281 caggttctcg tcaccctaaa cgctggcagg acatcgaaga gctgctggaa gcaggcattg
     5341 atgttttcac taccgtcaac gttcagcatc tggaaagtct gaatgatgtg gtcagcggcg
     5401 tcaccggaat tcaggtacgg gaaaccgtgc ccgatccttt tttcgatgcc gccgacgacg
     5461 tggttctggt ggacttgccc ccggacgatc tgcgccagcg gctgaaagaa ggcaaagtct
     5521 atattgccgg gcaggcggag cgcgccattg aacatttttt ccgcaaaggc aatctgatcg
     5581 ccctgcgcga actggcactg cgccgtactg ccgatcgcgt tgatgagcaa atgcgcgcct
     5641 ggcgggggca tcctagcgaa gagaaagtct ggcacacgcg cgacgcgatc cttttatgca
     5701 tcggccataa caccggtagt gagaagctgg tccgcgcggc ggcgcggctg gcgtcacggc
     5761 tgggtagcgt ctggcacgcg gtgtatgttg aaacccctgc cctgcatcgc ttaccggaga
     5821 aaaaacgtcg tgcaattctc agcgccttac gtctggcgca ggaactgggc gcggagacgg
     5881 caacactttc tgatccagcg gaagagaaag cggtagtgcg ttatgcccgt gaacataatc
     5941 tcggcaagat tattctcggt cgcccggcct cgcgccgctg gtggcgtcgg gaaacgtttg
     6001 ctgaccgact ggcgcgcatc gcccccgatc tcgatcaggt gctggtcgcg cttgatgaac
     6061 cacccgcccg cacgattaac aacgcgccgg atagccgctc ttttaaagac aagtggcggg
     6121 tacagattca gggatgcgtg gttgccgccg cgttatgcgc cgttatcacc ttaattgcca
     6181 tgcagtggct gatggcgttt gatgccgcca acctggtgat gctgtatctg cttggcgtgg
     6241 tggtggtggc gctattttat ggacgctggc cttcggtggt tgccaccgtc attaatgtgg
     6301 tgagtttcga tctctttttt atcgccccac gcggcacgct cgccgtctct gatgtgcaat
     6361 atctgctgac cttcgcggtg atgttaaccg tcgggctggt gatcgggaac cttactgctg
     6421 gcgtgcgtta tcaggcgcgg gtagcccgtt accgcgagca acgcacacgg cacttatatg
     6481 aaatgtcgaa agctctggcg gtgggccgca gtccgcagga catcgccgcc accagcgaac
     6541 aatttattgc ctccacgttt catgcccgca gtcaggtgtt gttgcccgat gacaacggta
     6601 aattgcagcc gttaacacat ccgcaaggaa tgacgccatg ggacgatgcc atcgcgcagt
     6661 ggagttttga taaaggcctg cctgcgggcg cgggcaccga cacgttaccc ggtgtaccgt
     6721 accagatttt gccgctaaaa agcggcgaga aaacctacgg gctggtggtg gtggagccgg
     6781 ggaatctgcg ccagttgatg atcccggaac agcagcgcct gctggagacg tttacgctgt
     6841 tagtcgccaa tgcccttgag cggctgacgc taaccgccag cgaagaacag gcgcggatgg
     6901 caagcgaacg tgaacagatc cgcaacgccc tgctggcagc gctttcgcat gatttacgca
     6961 cgccgcttac ggtgctgttt ggtcaggcag aaatcttaac gctcgatctg gcaagcgaag
     7021 gatcacccca cgcccgccag gccagcgaga ttcgtcagca tgtgctgaac actacccgac
     7081 tggtgaataa tctactggat atggcgcgaa ttcagtccgg cggctttaat ttgaagaaag
     7141 agtggttaac gctggaagaa gtagtcggca gcgcgctgca aatgctggaa ccgggtttat
     7201 cgtcgcccat caatctttct ctgccagaac cgctgacctt aatccacgtt gacgggccac
     7261 tctttgaacg ggtgctgatt aatctgctgg agaacgcggt gaaatatgcg ggtgcgcagg
     7321 ccgaaattgg tatcgatgcc cactttgagg gcgaaaatct acaactggat gtctgggata
     7381 acggccccgg tcttccgcca ggccaggagc agacgatatt tgataagttt gctcgcggga
     7441 ataaagagtc ggcggtgccg ggtgtcgggc ttgggttggc aatttgtcgg gcgattgtgg
     7501 atgtacacgg cggcaccatt accgcgttca accgaccgga aggtggtgcc tgttttcgtg
     7561 ttacacttcc ccagcaaacc gctcctgaac ttgaagactt tcatgaggat atgtgacaaa
     7621 cgttctgatt gttgaagatg aacaggctat tcgtcgcttt ctgcgcacgg cgctggaggg
     7681 cgacgggatg cgcgtctttg aggccgaaac tctgcaacgc ggcttgctgg aagcggcaac
     7741 ccgtaagcca gatttgatta ttctcgatct cggcctgccc gatggtgatg ggattgagtt
     7801 tatccgcgac ctgcgccagt ggagcccggt gccggtgatt gtgctttccg cacgcagcga
     7861 agagagcgac aagatcgccg cgctggatgc cggagcggat gattatctga gtaagccgtt
     7921 tggcattggc gaattgcagg cccgtctgcg cgtcgcttta cgccgccact ccgccaccgc
     7981 cgcgcccgat ccgctggtga aattttccga tgttaccgtc gatttagccg cccgcgtgat
     8041 tcaccggggt gaggaagagg tgcatctcac accaattgag ttccgcctgc tggcggtgct
     8101 gctcaacaat gccggaaaag tactcaccca gcgccagctc cttaaccagg tgtgggggcc
     8161 aaacgcggtc gaacacagtc actatttgcg tatttatatg ggacatctgc gacaaaaact
     8221 ggaacaggat ccggcccggc ctcgccactt cattactgaa accggtattg gatatcggtt
     8281 tatgctttga attgttattc aataaattcg cttaaatagt gatt
//
LOCUS       DAZPAC010000179         8298 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZPAC010000179 DAZPAC010000000
VERSION     DAZPAC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.179"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            96..968
                     /gene="rluB"
                     /locus_tag="ACHUPM_003363"
     CDS             96..968
                     /gene="rluB"
                     /locus_tag="ACHUPM_003363"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR0871053.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(1008..1598)
                     /gene="cobO"
                     /locus_tag="ACHUPM_003364"
     CDS             complement(1008..1598)
                     /gene="cobO"
                     /locus_tag="ACHUPM_003364"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR0871054.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(1595..2353)
                     /locus_tag="ACHUPM_003365"
     CDS             complement(1595..2353)
                     /locus_tag="ACHUPM_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR0871055.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2573..3622
                     /gene="sohB"
                     /locus_tag="ACHUPM_003366"
     CDS             2573..3622
                     /gene="sohB"
                     /locus_tag="ACHUPM_003366"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR0871056.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3658..3909)
                     /locus_tag="ACHUPM_003367"
     CDS             complement(3658..3909)
                     /locus_tag="ACHUPM_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR0871057.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            4289..6886
                     /gene="topA"
                     /locus_tag="ACHUPM_003368"
     CDS             4289..6886
                     /gene="topA"
                     /locus_tag="ACHUPM_003368"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR0871058.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            7096..8070
                     /gene="cysB"
                     /locus_tag="ACHUPM_003369"
     CDS             7096..8070
                     /gene="cysB"
                     /locus_tag="ACHUPM_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR0871059.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         2122 a         1974 c         2176 g         2026 t
ORIGIN      
        1 agactttact atcagtgagt cagatgtaag ttctgactca ctgttgcaca aaagcatatc
       61 agacgcctgg gaaggcgaca ccaaaaggaa gctctatgag cgaaaaatta cagaaagtgc
      121 tggcccgcgc tggccacggc tcccgccgtg aaatcgaatc tattattgaa gccggtcgtg
      181 tgagtgttga tggcaaaatt gctaaactcg gcgatcgcgt tgaagttacc cctgggctga
      241 aaattcgtat cgatggtcat ctgatttcgg tacgtgagtc cgctgaacaa atttgtcgcg
      301 ttctggccta ttacaaaccg gaaggtgagt tgtgtacccg taacgatccg gaagggcgtc
      361 cgacggtgtt tgaccgtctg ccaaagctgc gtggcgcacg ctggattgcc gtaggtcgtc
      421 tggacgttaa cacctgtggt ctgctgctgt tcaccaccga tggtgaactg gcaaaccgtt
      481 taatgcatcc aagccgtgaa gttgaacgtg aatacgccgt gcgtgtattt ggtcaggttg
      541 acgacgcgaa actgcgtgat ttgagtcgtg gcgtgcaact ggaagatggt ccggcagctt
      601 ttaaaaccat caagttcagc ggcggcgaag ggatcaacca gtggtacaac gtgactctga
      661 ccgaaggccg taaccgtgaa gttcgtcgtc tgtgggaagc ggttggtgtg caggtgagcc
      721 gcctgatccg tgttcgttac ggtgatatcc cactgccaaa aggtctgcca cgcggtggct
      781 ggacggagct ggacctcgcc cagactaact atctgcgcga actggtggag cttccgccag
      841 aaaccagctc taaagtcgct gtagaaaaag accgtcgtcg catgaaggct aatcagattc
      901 gtcgtgcggt gaaacgtcac agtcaggtga gcggcggtcg tcgttctggc gggcgtaata
      961 acggttaatc aaagtatgcc ggacgtcata tccggcattt ttacagatta ataatcaatc
     1021 cctatttgcg ccttaacacc ggcatcaaac gcatgtttga tggggcgcag ctcacttacc
     1081 gtatccgcca gttcaagaat atcccgatga cagcctcgac ccgtgataat caccgtctgt
     1141 tgatgtggac gttcatttaa cgcctgcacc acttcttcca gtggcaaata gtcatacgcc
     1201 accatatacg tcagttcatc aagcaaaacc atatccagtg aggaatcagc aagcatccgc
     1261 tttgcatgtt gccagacttc gcggcaggcg gcggtatcag actcgcggtt ttgtgtatcc
     1321 caggtaaagc ccgttgccat cacctgaaac tcaacgccat gtggctccag cagattgcgt
     1381 tcgccattag gccaggtgcc tttaataaac tgcacgacgc ccactttttt tccgtgacca
     1441 actgcgcgtg ttgccgtacc aaatgccgcg gtggttttgc cttttccatt gccggtaaag
     1501 acgatgataa taccgcgttc atcctgggcc tgggccacac gagcatctac tttttctttc
     1561 actcgctgct gacgctgttg gtagcgttca tcactcattg ggaaattcct ggtttacggc
     1621 ccggttgggc gtcaaaggtc atgccggttt tacggcggct gtcatcgccc atcagccaga
     1681 ggtagagcgg catgatatca gcgggtgttt taagtttctg tggatcttcg gtcgggaagg
     1741 cgcttgcgcg cattgcggtg cgcgtaccgc ctgggttaat gcagttgacg cgcaggcgct
     1801 gctgatattc atcggccagt acctgcatca tcccttcggt ggcaaatttc gacgctgcat
     1861 atgcacccca gttggctcgt ccctgacgcc caacgcttga tgaagtaaag accagtgaac
     1921 cggcgtccga tttgagtaat aaaggaagca gtgcctgggt gagcataaag gtggcattaa
     1981 cgttgacctg catgacgtcc tgccagacct gcggattttg ttcgctcatt gggcaaacat
     2041 cgccgagcaa tccggcatta tgcaaaacac catccagacg cggataatta acgacaatgc
     2101 gctgtgccag ttgttggcaa ttttcggacg tgcaggtcag caaatcgaga ataaaccact
     2161 gtggctgacg cccagtttct tcgtttatgt ggctggctac ctgacgtaat ttttcttcat
     2221 tacggcccaa cagaatcact gtcgcaccat agcgtgcata cgtcatcgcg gcttcacgac
     2281 caatcccatc gctggctccc gtcaccagga taatgcgatc attgagtaaa tcttgttttg
     2341 gctggtaatg catggctact cctcaacgac gttgtctgtc gcgctgccag tgtactttat
     2401 gactttgggg ctttatgcct gaaacagaag ggcatttcaa tcagcctggg gcgataacac
     2461 cgcaaaatgg atactttgtc atactttcgc tgcaataaca tctctgcgag acggcttaac
     2521 atgcctgttg taaactgtga gccaaagcgt tgtttaacca aggtggggac tcgtggaatt
     2581 gttgtctgaa tatggtttgt ttttggcgaa aatcgttacc gttgtgctag cgattgcggc
     2641 gattgccgcc attattgtca atgttgctca acgtaataaa cgccagcgtg gcgagttacg
     2701 ggtcaacaat ctcagcgaac agtataagga gatgaaagaa gaactggccg cggcgctgat
     2761 ggactcacat cagcaaaaac agtggcacaa agcgcagaag aaaaagcaca agcaagaagc
     2821 gaaagcagca aaagcgaaag ccaaactggg cgaggtggca actgacagta aacctcgcgt
     2881 ctgggtgctg gattttaaag gcagcatgga cgcccatgaa gtgaactcgc tacgtgaaga
     2941 gataacggcg gtactcgcag cattcaaacc gcaggatcag gttgtgctcc gtctggaaag
     3001 ccctggtggc atggtgcatg gttacgggtt ggcggcttcg cagctgcagc gtctgcgtga
     3061 taaaaacatt cctttaactg ttacggtaga caaagtcgct gccagcggcg gttacatgat
     3121 ggcctgtgtg gcggacaaaa ttgtttccgc accgtttgct attgtgggtt ccattggggt
     3181 ggtggcgcaa atgcccaact ttaaccgctt cctgaaaagc aaagatattg atatcgaact
     3241 gcacaccgcc gggcagtata agcgcacgct gaccttgctg ggtgaaaata ccgaagaagg
     3301 gcgggagaaa ttccgcgaag agctgaacga aacgcatcag ttatttaaag attttgtgaa
     3361 gcgtatgcgt ccgtctctgg atattgaaca ggtggcaacg ggtgaacact ggtacggaca
     3421 acaggcggta gagaaaggcc tggttgatga aatcaacacc agtgatgaag ttattcttag
     3481 cctgatggaa ggccgtgaag tggttaatgt acgctatatg cagcgtaaac gactcattga
     3541 ccgattcacc ggcagcgcgg cagagagcgc cgatcgattg ttgctacgct ggtggcagcg
     3601 gggtcaaaag ccattgatgt aaaagacaaa cgcgaggcta agacctcgcg ttttgcttta
     3661 atcaaccaga tgatattttt ctgaaagcac atgggccagg tgtttgaaca tattaaacac
     3721 cgcggtgctt ttggctgttg gcaatccttg ttcatctaaa aagtagtcgc cgataaatac
     3781 cagcacgcca ttacgctgcg tgacgccggt ggcttcaatc cctgcgagcg tatcctcatg
     3841 ctcacgaatg attttgttgg cctctttcaa cagcgtttcg cggtcgatgg gttgtgtctc
     3901 tttgttcatt atttactcct taaacaagga cattagtcta cgccaggcat ggcttgcaga
     3961 caaatatacc acgctggtgg caagagcgcc ttactggcaa ctttggattt tgcatgctaa
     4021 taaagttgcg tatcggattt tatcaggtac agtgtgacgc tttcgtcaat ctggcaatag
     4081 atttgcttga cattcgacca aaattccgtc gtgctatagc gcctgtaggc caagacctgt
     4141 taactcagtc acctgaattt tcgtgaacag agtcacgaca aggggttgat atccgcagag
     4201 aacgagtcca tatcggtaac tcgttgccag tggaaggttt atcaacgtgc gacgcattcc
     4261 tggaagaatc aaattaggta aggtgaatat gggtaaagct cttgtcatcg ttgagtcccc
     4321 ggcaaaagcc aaaacgatca acaagtatct gggtagtgac tacgtggtga aatccagcgt
     4381 cggtcacatc cgcgatttgc cgaccagtgg ctcagctgcc aaaaagagtg ccgactctac
     4441 ctccaccaag acggctaaaa agcctaaaaa ggatgaacgt ggcgctctcg tcaaccgtat
     4501 gggggttgac ccgtggcaca attgggaggc gcactatgaa gtgttgcctg gtaaagagaa
     4561 ggtcgtctct gaactgaaac aactggctga aaaagccgac cacatctatc tcgcaaccga
     4621 ccttgaccgc gaaggggaag ccattgcatg gcacctgcgg gaagtgattg ggggtgatga
     4681 tgcgcgctat agccgagtgg tgtttaacga aattactaaa aacgcgatcc gccaggcatt
     4741 taacaaaccg ggtgagctga atattgatcg tgttaatgcc cagcaggcgc gtcgctttat
     4801 ggaccgcgtg gtggggtata tggtttcgcc gctgctatgg aaaaagatcg ctcgtggtct
     4861 gtctgccggt cgtgtgcagt cggtggcggt tcgcctggtg gtcgagcgtg agcgtgaaat
     4921 taaagcgttc gtgccggaag agttctggga agtcgatgcc agcacgacca cgccatctgg
     4981 tgaagcgttg gcgttacagg tgactcatca gaacgacaaa ccgttccgtc cggtcaacaa
     5041 agaacaaact caggctgcgg taagtctgct ggaaaaagcg cgctacagcg tgctggaacg
     5101 tgaagacaaa ccgacaacca gtaaacctgg cgctcctttt attacctcta cgctgcaaca
     5161 agctgccagc acccgtcttg gatttggcgt gaaaaaaacc atgatgatgg cgcagcgttt
     5221 gtatgaagca ggctatatca cttacatgcg taccgactcc actaacctga gtcaggacgc
     5281 ggtaaatatg gttcgtggtt atatcagcga taattttggt aagaaatatc tgccggaaag
     5341 tccgaatcag tacgccagca aagaaaactc acaggaagcg cacgaagcga ttcgtccttc
     5401 tgacgtcaat gtgatggcgg aatcgctgaa ggatatggaa gcagatgcgc agaaactgta
     5461 ccagttaatc tggcgtcagt tcgtagcctg ccagatgacc ccagcgaaat atgactccac
     5521 gacgctgacc attggtgcgg gcgatttccg cctgaaagca cgcggtcgta ttttgcgttt
     5581 tgatggctgg acaaaagtga tgcctgcgtt gcgtaaaggc gatgaagatc gcatcttacc
     5641 agcagttaat aaaggcgatg ctctgacgct cgttgaactt acaccagccc agcactttac
     5701 caagccgcca gcccgtttca gtgaagcatc gctggttaaa gagctggaaa aacgcggtat
     5761 cggtcgtccg tctacctatg cgtcgatcat ttcgaccatt caggatcgtg gctacgtgcg
     5821 agtagaaaat cgtcgtttct atgcggaaaa aattggcgaa atcgtcaccg atcgccttga
     5881 agaaaatttc cgcgagttaa tgaactacga cttcaccgca cagatggaaa acagcctcga
     5941 cctggtggca aatcacgaag cagagtggaa agctgtactg gatcacttct tctcggattt
     6001 cacccagcag ttagataaag ctgaaaaaga tccggaagag ggtggtatgc gcccgaacca
     6061 gatggttctg accagcattg actgcccgac ttgtggtcgc aaaatgggga ttcgcacagc
     6121 gagcaccggg gtattccttg gctgttctgg ttatgcgctg ccgccgaaag agcgttgcaa
     6181 aaccaccatt aacctggtgc cggaaaacga agtgctgaac gtactggaag gcgaagacgc
     6241 tgaaaccaac gcgctgcgcg caaaacgtcg ttgcccgaaa tgcggcacgg cgatggacag
     6301 ctatctcatc gatccgaaac gtaagttgca tgtctgtggt aataacccaa cctgcgacgg
     6361 ttacgagatc gaagagggcg aattccgcat taaaggttat gacggcccga tcgttgagtg
     6421 tgaaaaatgt ggctctgaaa tgcacctgaa aatggggcgt ttcggtaaat acatggcctg
     6481 caccaacgaa gagtgtaaaa acacacgtaa gattttacgt aacggcgaag tggcaccacc
     6541 gaaagaagat ccggtgccat tacctgagct gccgtgcgaa aaatcagatg cttatttcgt
     6601 gctgcgtgac ggcgctgcgg gtgttttcct ggctgccaac actttcccga aatcgcgtga
     6661 aacgcgtgcg ccactggtgg aagagcttta tcgcttccgc gaccgtctgc cggaaaaact
     6721 gcgttatctg gccgatgcgc cacagcagga tccggaaggt aataagacca tggttcgctt
     6781 tagccgtaaa accaaacagc aatatgtctc ttcggaaaaa gacggaaagg cgactggctg
     6841 gtcagcattt tatgttgatg gcaaatgggt tgaaggaaaa aaataacctt taattctgtc
     6901 aggtttttat aaacaaaggg tcgcgaaagc ggcccttttt tattgcatat tattttttct
     6961 tcacacctat acactaaggc tataaatgat atagtggtta tagttagcac cttttttatt
     7021 attaaatcgt attagtcacc cgccaggtgt gacgaaaaaa cgatgttctg atggcgtcta
     7081 agtggatggt ttaacatgaa attacaacaa cttcgctata ttgttgaggt ggtcaatcat
     7141 aacctgaatg tctcatcaac agcggaagga ctttacacat cacaacccgg gatcagtaaa
     7201 caagtcagaa tgctggaaga cgagctaggc attcaaattt tttcccgaag cggcaagcac
     7261 ctgacgcagg taacgccagc agggcaagaa ataattcgta tcgctcgcga agtcctgtcg
     7321 aaagtcgatg ccataaaatc ggttgccgga gagcacacct ggccggataa aggctcactg
     7381 tatatcgcca ccacgcatac ccaggcacgc tacgcgttac caaacgttat caaaggcttt
     7441 attgagcgtt atccccgcgt ttctttgcat atgcaccagg gctcgccgac acaaattgct
     7501 gatgccgtct ctaaaggcaa tgctgatttc actatcgcca cagaagcgct gcatctgtat
     7561 gaagatttag tgatgttacc gtgctaccac tggaatcggg ctattgtagt cactccggat
     7621 cacccgctgg caggcaaaaa agccattacc attgaagaac tggcgcaata tccgttggtg
     7681 acatatacct tcggctttac cggacgttca gaactggata ctgcctttaa ccgcgcaggg
     7741 ttaacgccgc gtatcgtctt cacggcaacg gatgctgacg tcattaaaac ttacgtccgg
     7801 ttagggctgg gggtaggggt cattgccagc atggcggtgg atccggtcgc cgatcccgac
     7861 cttgtgcgtg ttgatgctca cgatatcttc agccacagta caaccaaaat tggttttcgc
     7921 cgtagtactt tcttgcgcag ttatatgtat gatttcattc agcgttttgc accgcattta
     7981 acgcgtgatg tcgttgatgc ggctgtcgca ttgcgctcta atgaagaaat tgaggtcatg
     8041 tttaaagata taaaactgcc ggaaaaataa tttcttgcgt tattttcggc accttttatg
     8101 tagcgaaggt gccggaatat attctctttt gttacttatt tctataccgt gcacaatatt
     8161 tttatccctc atatttatag ggtaaattac ctgtaaagta gtgtctaatt acccgcctcg
     8221 caaactccag ttttcaaata tctatttcag gtcaaaagat tgaattattc actggagacg
     8281 attcgtaaat tcgctgga
//
LOCUS       DAZPAC010000180         8298 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZPAC010000180 DAZPAC010000000
VERSION     DAZPAC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.180"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            57..719
                     /locus_tag="ACHUPM_003370"
     CDS             57..719
                     /locus_tag="ACHUPM_003370"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR0871060.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCNLPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNGLTIRSPFPSAVVVFKKEPRKHEITQTVCCGD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKCIADALRMPLSTSYRIAQR
                     LS"
     gene            1102..2055
                     /locus_tag="ACHUPM_003371"
     CDS             1102..2055
                     /locus_tag="ACHUPM_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0871061.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEEELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            2055..2351
                     /locus_tag="ACHUPM_003372"
     CDS             2055..2351
                     /locus_tag="ACHUPM_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871062.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            2675..2965
                     /locus_tag="ACHUPM_003373"
     CDS             2675..2965
                     /locus_tag="ACHUPM_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871063.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            3186..3401
                     /locus_tag="ACHUPM_003374"
     CDS             3186..3401
                     /locus_tag="ACHUPM_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871064.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            3548..3853
                     /locus_tag="ACHUPM_003375"
     CDS             3548..3853
                     /locus_tag="ACHUPM_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871065.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            4329..5039
                     /locus_tag="ACHUPM_003376"
     CDS             4329..5039
                     /locus_tag="ACHUPM_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0871066.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            5064..5270
                     /locus_tag="ACHUPM_003377"
     CDS             5064..5270
                     /locus_tag="ACHUPM_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871067.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            5369..7549
                     /locus_tag="ACHUPM_003378"
     CDS             5369..7549
                     /locus_tag="ACHUPM_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR0871068.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            7706..8298
                     /locus_tag="ACHUPM_003379"
                     /pseudo
     CDS             7706..8298
                     /locus_tag="ACHUPM_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640578.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
BASE COUNT         2711 a         1492 c         1771 g         2324 t
ORIGIN      
        1 tcttaaaaga gagtaaacct tgtcaagagt gattgcaata aaatttttta tttcttatgt
       61 caattgagca tattttaatc gggaaacatc atgggggttc tgattactgg agaactccat
      121 tgttgttatt taagaactta caccgtgaat ttggttttac gctagatggt gctgcaactg
      181 agcatgacac gctgttacca cgattcacca atgatataag ccgtcaatca tgggttagtg
      241 aaaaggtctt ctgtaacctg ccgtacagcg atattccgtc gttcctctta aaagcgtctg
      301 aagcggattt agtagctttc ctgataccgc atagagccaa caccagctac tggcttaggc
      361 acatttacag caataaccac tgtcatgaaa tccgtattct tcatcgtgca gttaaatacc
      421 ttccaccagc aggccataac gggcttacta ttcgttcacc gtttcctagt gcagtcgttg
      481 tttttaaaaa agaaccacgt aagcacgaaa ttacacagac ggtttgctgt ggtgacacgc
      541 tattaccgtt aaccatcatt aacagaggag gcttgagagg aagacccacc atttacccac
      601 ctgagacgct cgacagcttc ataaaactat acaggcaagg aaaacccatt aagtgcattg
      661 cagatgctct cagaatgcct ctgagcacgt catacaggat tgctcaaagg ttgagctaaa
      721 attctcataa atatggtcat ttacaagata ggtgattcag atattaagaa gtatccctgt
      781 gaacaataaa tgaccaatca gtaattattc tgtgaaataa ttgttgcaat ttataatcac
      841 atctgttata gtttttatat ggggtgataa aagaactcag aacattaaga ggttacaacg
      901 ttgggaggcg tcagggtatt gacagatttt cacggcctct tattcaatca aggcttttcc
      961 ttttgctgtt aatactcgcg taatcgctca ggcttctgag ttcctttatc agtccatctt
     1021 ctaattaatt cactccctct ggggttggtc actatacgtg gtcagccctt tctttgtttt
     1081 atttttaaaa aggaaaatat aatggaaaaa ttccacatgt ttgatgaccg tatttatcag
     1141 atgactaatg gcgttaaagc tgccgaccgt attatttaca ctgtcatgtt gaatgaacaa
     1201 aagttttatc acgataaggg gcaatattat accccttcat atgctcatct tggtcagtat
     1261 gctggaatca caagaacatc cgtgattgca tgtatgaaac aacttgaggc acatggttta
     1321 atcaagaaaa ctaacgttgc aagcaatcag tgtgttatta aagtttattc acttgatgaa
     1381 gtaccggagg ttttaacaga gcttacagct aaggaagcca gaaaggcaaa gctggagtct
     1441 aaagttactc agtcagtcaa gaaagatgac ccactgagtc aaaattcttt acccactgag
     1501 tcaaaatttt ttactcagtc agtcaaaatc tttgacacgt ataataacag taataacaag
     1561 aaagaactta ttaataacaa taaagaagat gactttgaag aagaatattt taagaaggga
     1621 gcttcgctct gtgattcaca tgaatgtgat tctcttggta ttgaagaggc tattaaagag
     1681 actgttaaaa catctgactt agatgacctt gatagtgaag ctgctaaaga ggtcgttaaa
     1741 aaggaagata aaatctctga tgtgattgct agtagtgacc ataaaaacac acagtgcccc
     1801 gccactgatg atgaattacc ggatgttgac cttgatgatg aagaagaccc tgaagaggaa
     1861 ttgaaacgtg aacagcgtaa agcagaaatt aaagaaagat tcagaaatgc gaagctaaag
     1921 aacaaggtta gtgacctcga tgactttgat gatagtgacg atgattttac ttttacacca
     1981 aaaaagaaaa ctcacaccga gccaaagaaa gaaaaagtta aagtcagacg tgtttatggt
     2041 ggaggaatgt tttaatgaaa acttataatg gcgagagaaa ataccgtgac tatttactca
     2101 gacgttacag catttctcgt gaaggttact tgatgaaaga tactcacggg gaagtttacc
     2161 ggattcgacc gaagaaagaa gggagggatt attatttcgt cgatgatgtg acagatttaa
     2221 agattgacgc tttgaagttt gcggtgctgt atcattacga tttatgggat tcagtccacc
     2281 agctacgatt aaaagacggt gaccccggta atctgagggc caccaatatt atcaagggaa
     2341 agtgccggta aggccaccag ctatttattt actgaaatct ctatctcaac acgaagaagt
     2401 ttaagtcgaa ttgtaagctt tgtctttttt gtaatgactc gattcataac gcaaaacacc
     2461 aatagcacaa taaataccgc aaagcccaac gagctaaaac gatgagattc aataattgct
     2521 gtgaaaaaat catgcattaa ttttcctttt gtgaaaggaa acagcacctg tatcagcccc
     2581 tacaaccata gtatcatgta aatgaggtac tgacaatatg ttaaatagat gttaagattg
     2641 tagtatgagt aagagattta aggagatttt tacaatgact cacacagtta cgcgcctttt
     2701 gccagaggca tttgaacgac tggcagcaat caaagccaaa tatcccgata tgaagcacac
     2761 agcgcttgtt ggggagattt taaaagagtc ggtaaacctt agccctttcg ataacgttaa
     2821 gggcgtcgca gagggttcta aggccatcag aatcacaacg ggccaactgg aactactcaa
     2881 agcacagtcc gcagtagcag gttgctcaat gattcaggtg ttaagcgcta aggtccatca
     2941 actggcggat agattggagg tttaatgtgt tataggttcc aatattccac acttggccgc
     3001 cacctaatca gacagaattt agcagatgct gaatttgaag atgtgaccct caaagcaaac
     3061 aaacgattaa aaccctctca aatccaccag cttaaacacg aattaaagcc actggtaata
     3121 ctcgactata acgaccctga acagcttgag cagggaatta agcttatttc tgaatggggt
     3181 gcttgatgaa aactatcaca gtacaacttc agactaataa agcatttcgt tattttgaaa
     3241 acttgcttga gttatacgaa ggatggggaa gtatccacgg caaagatgat atctatcttc
     3301 acttgtcagc ccctaactat tctctgaaga ctccggttaa gcaaagctgg ttaaaagact
     3361 acggtcacca gatggggcta ttagtctcag acttgagcta aaaacagcaa aacatatctc
     3421 tcaggcttta ccagacgcac cagaaacgtt tttaagcccc taatcagggt aacttaggga
     3481 atcgccttaa gtggtctaaa aaaggggtta aaacgtctta gaatgcgata caggaaggat
     3541 tttaaaaatg aattacccaa tgcaaattat ggttgaccaa gaaaccatga attggttgat
     3601 tgacctgaac aggtcgggct ttaatatcta tttcatgcat tgttttgaag cgtcaacgta
     3661 tattgacaag catataatca acgaaattaa acttggtgac tatgttcaca agctggaagt
     3721 aacagaccca tacaataagc gctttatcat tgaagagtgc aatcgacttg atttaagccc
     3781 tgagcaatta ttgaagctga atgtattctt gacctatcag aatgaattaa acccttacag
     3841 cgaggtgatg taatgacact cgttattctc tggatgactt tagggttatt tgttggttgc
     3901 tgggtaggta atacggtaaa taagaattga tgttaccaat ctgttaaaaa gctgttgaca
     3961 aataacctta agcatgagat gatggtaata gggaagagca actcttcctg aattaaacta
     4021 ttaatcattc tacatcaatg atagttattc tgaaaggtgc aatgcttttc gctacatgac
     4081 ctttcaggtt agtctgagag gtcatctcga aaagtactct tgttcattcg tttgaatcct
     4141 ttttgaatcc tttagatgtt ttaatttctt caccttatga atggcccttt ataagaaatt
     4201 atctagggct attcagcttt taaaaagcat acttggcaaa ccgcctttta agtctcttct
     4261 aagtttagat aggctgtcag gtgtgctttt taaaaactga aacacttccg cttagaagag
     4321 gaaaagcaat gacaaatgat atttacatta atggcaaagt gattgatagc ttcagtgact
     4381 ggtcgccaca gactgaacat ccagctatca ctattcagcg taaagagcat gacaagcgta
     4441 ttgagcttga gcaatcgcta aagatgtcac caaaaacaac catctttgtg tctcctgagc
     4501 ctcaagagat gcctgatatt tgcaagagtg aagcattact tgagcttgaa aagaaatatt
     4561 accaactgct gaaagaacag cgccttagat tagatgacgc ttacagcaaa gttaccttga
     4621 tgcaatcagc tattgagccg tcagaatttg agattcagga tgaattgtca cagaaaccat
     4681 ttgtctatta ccaatacgaa gataatgatg gttttggtac ttttcaagaa aacgtcgcag
     4741 ccgtgattag tagtcttcct gctggacatc gaattgtgaa gatggttaag gcatcaagag
     4801 gtgctggtca gtttatttac atgactaata agagcgatga agaactcgct gaaattgcta
     4861 gacagaacat cttggcatca cgtaacaagc aacttgataa tgttaagttg tatctctcaa
     4921 gagagcttca ggctatgaaa gagcttgtga aagaatatga agatgctaag aagattgcta
     4981 tgcaggctga tattgagcag cttacgaaga tttctagcaa atatgcaaag gctatttaat
     5041 taataattaa aaggtggtgg tgaatgattg atttaacaga acgctatgag cttattaaag
     5101 caattagtga acgattgaaa gaacaaccca acaaaccaaa acgaatcaaa tcattcacct
     5161 cctctaagca aaagaataga atcaggaaaa tagctaagtg gtgtcttgat aaattaccaa
     5221 gtgattgtca gattgttggt gaagatggta attattatct ggttaggtaa caagattggt
     5281 tgggtgaaaa tctacgggag agattatcga gtgatatcgg tagtctttcc ctttttcgta
     5341 tatgaacact taaagaggtg aaacatgaat gagcaaattc acagttgata gttttatcgt
     5401 tgaacttagt ttcagtgaaa acgttattaa gggcttgcaa agagttgaaa aggcagcttt
     5461 gcagtcagca caaagaattg aacgcaattt gaatagagcg tttaaggtcg ataccaagca
     5521 acttgacagc aatctgacaa gttctttagg gcagcttgaa aggaagttta ataagacctt
     5581 tgacaagatt gagcagagag caagaagcac aaaagcattt cagtttgcca ccagatttaa
     5641 tgacactgtt aatcctccaa aacaatcaag gcaaccacga attagcggaa acagagcaat
     5701 cacagcagcg catagcgtga atatgtctaa gcttggtgat attaatccgc tgatggctaa
     5761 aatgtttaaa gcacagtatt acagcttaag cggtaaggct ggtaatattg gaagtgaaaa
     5821 gtttaacgct gaacttgcaa aattaaatca atctcttcgt gaaacgttga ataaactcag
     5881 gtcacagaca aagaccgcca gcattaacaa tacaagcgac gcatttaata atcttgcaag
     5941 caatgcgatt aaattatctg gtgcatttta ctctgtgatg ggtgctctta acgcttacaa
     6001 ggccattatg aatgcaggtc ttaaacgaga ttccgcacag agagccgcta agtttgtact
     6061 tggtgataaa gcatctgaag ctgaggtatt tattcgaaat ttagcagaca agacaggtct
     6121 taatatctct gaagggctat caagctacgc taagtttgca gcaggtgctc agggttctat
     6181 gtcacaggaa cagacacagg agctatttgg taatgcaacg gctatgagtc gcttaatggg
     6241 gctttctaac gatgagctaa acggcatttt gagagcattt gagcagatgg cgagcaaggg
     6301 caagattcag gctgaggaat tacgtgggca gttaggtgac cgtatggctg gtgcatttaa
     6361 gctattcgct gaagcattgg gtatgactgc tacagagctt gatgcagcca tgaaaaacgg
     6421 gaaggtactt tccgctgata ctctccctaa agtcgctaaa cagatgggct tgatgattga
     6481 taaagcgggt ggctgggctg aagtcactaa gagcactcag acagctttag gtaagttagc
     6541 aaacaattgg gatgatacga tggtgaaaat cttctcaggt tcacaggatg agctaaacgg
     6601 cttcttatcc agcctgagta acctgcttag tgaaatgggg atgagttctt ctatcgctgg
     6661 tgatgctatc ggtggactta tcgacatgct taaagctggt gtagatgata ttcgcgtatt
     6721 caataacacc attgaaggct ggattttaca gactaagaag ttctattaca gccttgatga
     6781 cactaaacga aaactacttg atgaagtcgg tgatggtttt atcaacttcg tcaaaggtct
     6841 tgctatagcg ttatctgcaa agacgctatt cagtgctact acaggggtta tgaacctgac
     6901 aagagcaatc acaacgttag gtacaagagc taatcaggtg gctggtcagg tagctactgg
     6961 taaaggtggt ggaaaactga aaggtgtagc tggtggtgta ggtagtgctc ttgcaattgg
     7021 ttatgctgat gacggttatg aaacagcgtt agcattagct tcaatgattc cacaaatcag
     7081 aggcgtcact cttggtttat atgcattgaa gaaagcactg gattttatga atcaggaagt
     7141 agtcaaaaac gctaatatgc atccgtctgg ggtaggtgtg ggtagtgact ttaatcccgt
     7201 gtattctggt gaccctaata aaccgaatat gattaacgaa ggatggagta gaatttttag
     7261 ttcaatgggt gaattgttta acaatgccac aatgaatatt gcaagattca atcatccaga
     7321 gttaaacaac attaagcaag attcagcatt aacgagtgca gatattcagg gtctgaggga
     7381 tgaaattagt gctttgagta agcgtattca ggagccagtg aaagtttcac ttggtggtca
     7441 agttgtcgtt cgaccagatg aaccatcttt ctttactttc aatcaatcag ctaatgacca
     7501 gttcacgcag gatatgctct taagttcaag ctatccagag gaagaatgat ttaacggtgt
     7561 tctacgtgaa taaagcaaag ttaataccca caccaaaaat tatttttcac gtagctcacg
     7621 cttaacagag aaaatacata tatatcaaag gcttggttaa aaatgattaa aatacactaa
     7681 gcctttgaaa tcaaaggaga atttaatgat ttacactaat cagcttcgtt acagtggcaa
     7741 agatggcatc tatttccact taagagacaa tgtagatgcc ttcttaacct tatcagcaac
     7801 tgaaaacatg gaatttgata gccctatgca ggtgactaca cagaacatgc aattatgaag
     7861 gaagcttatt attaactcgt cagggtcaat tagtagaaaa cttcattgac actctggaaa
     7921 actggcgtga ccagaagcag attatttctg ttatctgtaa agatggaatt aaaattgacg
     7981 attccattat taccaacttt aaagcctcta aagatgttac tatctctaac ggtcttcgta
     8041 tccagctaac ttttcaggaa attaacttta aagcaatcgt aggtcaaact gatatttcag
     8101 cagccactgg caaaacggct accacaaatg acggtggtgt gaccagtaag aagaatgcag
     8161 gtaacgtgac cactgaacgc aataacgcgc ctatgctatc gtgcaaagag ttaatgaata
     8221 ttagcgatgt atccggttac gctccagcta tcagaaatga tattctcgat gctcagatta
     8281 aatgtaataa ggcttgga
//
LOCUS       DAZPAC010000181         8264 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZPAC010000181 DAZPAC010000000
VERSION     DAZPAC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.181"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            44..568
                     /gene="radC"
                     /locus_tag="ACHUPM_003380"
     CDS             44..568
                     /gene="radC"
                     /locus_tag="ACHUPM_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR0871069.1"
                     /translation="MNKLESLPGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYN
                     VRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEV
                     HPREIIREAIKINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVI
                     GRGEYVSFAERGWI"
     gene            785..1021
                     /gene="rpmB"
                     /locus_tag="ACHUPM_003381"
     CDS             785..1021
                     /gene="rpmB"
                     /locus_tag="ACHUPM_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR0871070.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            1042..1209
                     /gene="rpmG"
                     /locus_tag="ACHUPM_003382"
     CDS             1042..1209
                     /gene="rpmG"
                     /locus_tag="ACHUPM_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR0871071.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1307..2116
                     /gene="mutM"
                     /locus_tag="ACHUPM_003383"
     CDS             1307..2116
                     /gene="mutM"
                     /locus_tag="ACHUPM_003383"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR0871072.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="ACHUPM_003384"
     CDS             complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="ACHUPM_003384"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR0871073.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="ACHUPM_003385"
     CDS             complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="ACHUPM_003385"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR0871074.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4332..5390
                     /gene="rfaQ"
                     /locus_tag="ACHUPM_003386"
     CDS             4332..5390
                     /gene="rfaQ"
                     /locus_tag="ACHUPM_003386"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR0871075.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5387..6511
                     /locus_tag="ACHUPM_003387"
     CDS             5387..6511
                     /locus_tag="ACHUPM_003387"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR0871076.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6504..7301
                     /gene="rfaP"
                     /locus_tag="ACHUPM_003388"
     CDS             6504..7301
                     /gene="rfaP"
                     /locus_tag="ACHUPM_003388"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR0871077.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7317..>8264
                     /gene="waaO"
                     /locus_tag="ACHUPM_003389"
                     /gene_synonym="rfaI"
     CDS             7317..>8264
                     /gene="waaO"
                     /locus_tag="ACHUPM_003389"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFR0871078.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNF"
BASE COUNT         2128 a         1902 c         2083 g         2151 t
ORIGIN      
        1 ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc
       61 attacctggc ttgttaacct ctgaatatga acaatttagt ggcgttcatg gaattggcgt
      121 agcgaaattt gcccagttaa aggggattgc tgaactggcg cggcgttact acaacgtgcg
      181 gatgcgtgaa gaaagccctt tactcagccc ggagatgacg cgggaatttt tacaaagcca
      241 gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactcac aacaccgggt
      301 tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga
      361 aattatccgg gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc
      421 ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag
      481 ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt
      541 ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc
      601 tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg
      661 ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta
      721 aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat
      781 agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc
      841 ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg
      901 ggttgagagc gaaaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat
      961 cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta
     1021 agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct
     1081 tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa
     1141 ctggaactga aaaaattcga tccagttgtt cgccagcacg tgatctacaa agaagcgaaa
     1201 atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat
     1261 ctgcttgccc ccatattgat tgcatctgtt cattcctgga gatgctatgc ctgaattacc
     1321 cgaagttgaa accagccgtc gcggcataga accgcatctc gttggtgcaa ccattcttca
     1381 tgcggtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag
     1441 cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ctgctgctgg agctgcctga
     1501 gggctggatt atcattcatt tggggatgtc tggcagcctg cgcatccttc cagaagaact
     1561 tccccctgaa aagcatgacc atgtggattt ggtgatgagc aacggcaaag tgctgcgcta
     1621 caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcacaacgt
     1681 gctggctcat cttggaccgg agccgcttag cgacgatttc aatggtgagt atctgcatca
     1741 gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt
     1801 aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg
     1861 gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt
     1921 gttgctgcgt tcgattgagc agggtggtac aacgctgaaa gattttctgc aaagtgatgg
     1981 taaaccgggc tatttcgcgc aggaattgca ggtttacgga cgaaaaggtg agccgtgtcg
     2041 ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg
     2101 gcagtgccag aagtaattca tacgcgccgg atggcatacc atccggcata aacgctacgc
     2161 taacttcgcc accagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg
     2221 atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat
     2281 cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc
     2341 atattcaaaa tccgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg
     2401 ggcgaagttc gccatcaaat cactaaaccc aaccacttcc acgttcccca gatgcgcggt
     2461 tgcctgctgt gtcagcgcca cacgctcttc cagggtaaac atcggttttt tactggggct
     2521 ggcggcaatc gccagaataa cgtgatcgaa catttgcgtg gcgcgcgtca cgatatcgat
     2581 atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac
     2641 ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc
     2701 cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt
     2761 cggtgagtaa agaggaaacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc
     2821 cgcttgcctg ctccagccgc gcgcaaatgt ctttgaaatt aaaagtatgc ggccccatca
     2881 ataccggaat agcgtgtgcg gcagcttcca gaggattatg ccctccacgt tcaaccagtg
     2941 aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat
     3001 cgccaaccac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc
     3061 ttagtccagc ctggcggaca aggttaatcg catccgggaa gcgttctgga tgacggggta
     3121 ccaggatgag caataaattc gggaattgct gtaataatgc ctgatgtgcg gcgatcacca
     3181 cactctcttc accttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact
     3241 ggcggcgcag cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca
     3301 ggctaccggt aacggttacc tggttatttt ttgcgcccag cgccacaaaa cgtgcaccat
     3361 cttcttcatt ttgcgcagca atcagcgtaa tacgacgcag taagcggcgg acgaatttac
     3421 ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg
     3481 gaattttacg tttatgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca
     3541 acaccagttt ggggtcgact ttattcagga aacggttgag cgcatcgggc aggtcatacg
     3601 gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg
     3661 gcgtcatggt tgtaacggta atcggtaaat caggataacg atggcgcagc gcgcgcacca
     3721 acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt
     3781 ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct
     3841 tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg
     3901 tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta
     3961 tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc
     4021 aattcttgct gaacatctat actttttttg tatttttatg cgattcattg atttctgctg
     4081 tacgtgcagg aaggaatcgc agagcaacag tgaaagaata tggttgctaa tgattttatt
     4141 tgattgattt ctttattgaa aatcaacaat gtgcttaaaa agcattcggg tcggattgcc
     4201 gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg
     4261 ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa
     4321 aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg
     4381 gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa
     4441 aaatcgatgt gctgctttat caggacacca ccccgatcct gtctgaaaat ccagagatta
     4501 acgcgctcta cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt
     4561 ttcatctcat caaggtatta cgtgccaata agtatgacct tatcgtcaat cttaccgatc
     4621 aatggatggt tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt
     4681 atcatcatcg gcagtctgct ttttggcgta aaagtttcac ccatttagtg ccgttgcagg
     4741 gtggaaatgt ggtggaaagt aacttatccg tgctgacacc attgggactt gattcgttgg
     4801 tgaagcagac aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg
     4861 atcacgctgg tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca
     4921 aatgctggga caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg
     4981 aagtcgttct gacgtccggc ccagataaag acgatctggc ctgcgtcaat gaaattgcgc
     5041 agggatgcca gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg
     5101 gtgcgttaat cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcgcatattg
     5161 ccgctgcagt taatacgccg ctgatatcgc tgtttggtgc gacagaccat attttctggc
     5221 gtccttggtc aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc
     5281 gcgatcagcg tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg
     5341 ctgctgtcga taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt
     5401 ttgtttatat aaatattttc cctttggcgg tttgcagcgc gattttatgc gtattgctca
     5461 gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg
     5521 ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa
     5581 tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt
     5641 tggattcaac aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga
     5701 gaaagtcgcg caggaaaaag gctttttcta tcacctgacg tcacgttatc gccattatgc
     5761 cgcctttgag cgggcaacct tcgaacaggg caagccgaca cagctgctga tgctgacaga
     5821 taagcaaatc gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct
     5881 gccaccgggg atttatcctg atcgtaaata tagccagcag ccagctaata gccgtgaaat
     5941 cttccgtaag aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga
     6001 cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct
     6061 gcgccacaac acattgctat atgttgttgg acaggataag ccgcgaaaat ttgaggcgct
     6121 ggcagaaaaa cgcggcgtgc gcagtaatgt tcacttcttc tcggggcgca acgatgtctc
     6181 ggaattaatg gcggcggcgg atttattact gcatcctgcc taccaggaag cagcgggaat
     6241 tgtgctactg gaagcgatta ctgcaggatt accggtacta acaacagccg tttgtggcta
     6301 tgcgcattat attgtcgacg ccaattgcgg cgaggctatt gctgagccat tccgccagga
     6361 aacattgaat gagattttac gcaaagcgtt aacgcaatct tcattgcgcc aggcctgggc
     6421 ggaaaatgcg cgacattatg ctgatacaca agatttatac agtctgccag agaaagcggc
     6481 ggatatcata acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc
     6541 gcggtaaaga tccttttgag gaagttaaaa ccttgcaggg tgaggttttt cgtgaactgg
     6601 aaactcgccg cactctgcga tttgaaatgg cgggcaaaag ctattttctc aaatggcatc
     6661 gcggcacgac cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag
     6721 gcgcagaccg cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgt
     6781 atggggtggc atttggcgaa aaaggtatta atccgctgag cagaacctcg tttattatta
     6841 ccgaagatct gaccccaacc atcagtctgg aagattactg tgctgactgg gcgaccaacc
     6901 caccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcgcgata
     6961 tgcatgctgt gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc
     7021 ctttttccgg taaggaagag gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc
     7081 ttcgcacgcg cgttccacgt cgttggcgcg ataaagatct tattgggctt tatttttctt
     7141 cgatgaatat cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgtcg
     7201 ccccgcttaa agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag
     7261 ccacaaaaat cagggaaaga acgattcgaa aatcgttgta atttccggag agaaatatga
     7321 gtgcccacta ttttaatcca caagagatga tcaataagac aatcatcttc gatgaaaggc
     7381 cagcggcgtc agtggcatca tcattccatg ttgcttatgg cattgataaa aactttcttt
     7441 ttggttgtgg tgtttcaatc acgtcagttt tgttacataa caacgacgtg agttttgttt
     7501 tccacgtttt tattgatgat atccctgaag ccgatatcca gcgtttagcc caattggcga
     7561 aaagctatcg tacctgtatc cagatccatc tggtaaattg tgaacggctt aaggcattac
     7621 cgacgaccaa aaattggtct attgccatgt atttccgttt tgtaattgca gattacttta
     7681 ttgatcaaca agataagatc ctttacctgg atgctgatat cgcctgtcag ggaaacttaa
     7741 agccgctgat aacaatggat cttgccaata acgttgctgc tgttgttact gaacgcgatg
     7801 ctaactggtg gtcgttacgg ggtcaaagtc tgcagtgtaa tgaacttgaa aagggttact
     7861 ttaattcagg tgtcctgtta attaatacac tagcgtgggc gcaggagtcc gtttctgcta
     7921 aagcgatgtc gatgcttgct gataaagccg tcgtttcccg tttaacctat atggatcaag
     7981 atatacttaa tcttatcctg tcagggaaag ttaaattcat tgatgctaaa tacaatactc
     8041 aatttagttt aaattatgaa ttaaaaaaat catttgtttg tccaattaat gatgaaaccg
     8101 tattaattca ttatgtcggc ccgacaaaac cctggcatta ctgggccggt tatccaagtg
     8161 cgcaaccttt tatcaacgcc aaagaagcat cgccctggaa aaatgaaccg tttatgcggc
     8221 caattaattc aaactatgct cgttattgcg ccaagcataa tttt
//
LOCUS       DAZPAC010000182         8214 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZPAC010000182 DAZPAC010000000
VERSION     DAZPAC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.182"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..100
                     /gene="mgtT"
                     /locus_tag="ACHUPM_003390"
     CDS             <1..100
                     /gene="mgtT"
                     /locus_tag="ACHUPM_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFR0871079.1"
                     /translation="GDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="ACHUPM_003391"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="ACHUPM_003391"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFR0871080.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="ACHUPM_003392"
     CDS             complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="ACHUPM_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR0871081.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2028..2546
                     /locus_tag="ACHUPM_003393"
     CDS             2028..2546
                     /locus_tag="ACHUPM_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR0871082.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="ACHUPM_003394"
     CDS             complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="ACHUPM_003394"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR0871083.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4773..5519
                     /gene="ydfG"
                     /locus_tag="ACHUPM_003395"
     CDS             4773..5519
                     /gene="ydfG"
                     /locus_tag="ACHUPM_003395"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR0871084.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5608..6294
                     /gene="rspR"
                     /locus_tag="ACHUPM_003396"
                     /gene_synonym="ydfH"
     CDS             5608..6294
                     /gene="rspR"
                     /locus_tag="ACHUPM_003396"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR0871085.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6471..6674
                     /gene="ydfZ"
                     /locus_tag="ACHUPM_003397"
     CDS             6471..6674
                     /gene="ydfZ"
                     /locus_tag="ACHUPM_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR0871086.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6710..8170)
                     /locus_tag="ACHUPM_003398"
     CDS             complement(6710..8170)
                     /locus_tag="ACHUPM_003398"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0871087.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2171 a         2055 c         1870 g         2118 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt
     1201 catctctgag ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc
     1261 tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca aatagcataa cccaataccc
     1321 taatggccca gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac
     1381 ccgaacgacg gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc
     1441 cggctgaact tctcgtccat caaagacggc cgcaggaata acaacattaa tttcaccgct
     1501 cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga
     1561 aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg gtcatttttt ttgcatcatc
     1621 ggttccgcga tagccatctt ctattgcatg aggcggcggt ggcgctgcat cctgttttaa
     1681 accgccctgg tcatctgcca acgcataagg catgacaaga aaacttgcta atacaatgga
     1741 ctgaaatttc atactaactc cttaattgcg tttggtttga cttattaagt ctggttgcta
     1801 tttttataat tgccaaataa gaatactgcc aattgttata aggcatttaa aatcagccca
     1861 ctagctgtca aatattcaga gaatttgact cactaaagtt aagaagattg aagagtctta
     1921 aacatgtttt cagaataatc ggatttatat gtttgaaaat tattatattg gacgagcata
     1981 cagaaaaagc aaatcacctt tacatataat agcgtggaca aaaaacagtg aacattaata
     2041 aagataaaat tgtacaactc gcagataccg acactattga aaacctgaca tccgcgttga
     2101 gtcaaagact tatcgcggat caattacgct taactaccgc cgaatcatgc accggcggta
     2161 agttggctag cgccctgtgt gcagctgaag atacacccaa attttacggt gcaggctttg
     2221 ttactttcac cgatcaggca aagatgaaaa tcctcagcgt aagccagcag tctcttgaac
     2281 gatattctgc ggtgagtgag aaagtggcag cagaaatggc aacaggtgcc atagagcgtg
     2341 cggatgctga tgtcagtatt gccattaccg gctacggcgg accggagggc ggtgaagatg
     2401 gtacgccagc gggtaccgtc tggttcgcgt ggcatattaa aggccagaac tacactgcgg
     2461 ttatgcattt tgctggcgac tgcgaaacgg tattagcttt agcggtgagg tttgccctcg
     2521 cccagctgct gcaattactg ctataaccag gctggcctgg cgatatcccc aggccagcca
     2581 ttggtggtgt ttatatgttc aagccacgat gttgcagcat cggcataatc tgaggagcct
     2641 taccgcgcca ttgtcgatgc aggcgttcca gatcttcgct gttacctctg gaaaggatcg
     2701 cctcgcgaaa acgctgccca ttttcacgcg ttaatccgcc ctgctcaaca aaccactggt
     2761 aaccatcatc ggccaacatt tgcgtccaca gataagcgta ataacctgca gcatatccgc
     2821 caccaaaaat atgggcgaaa taactgctgc gatagcgtgg cggtatagca ggaagatcca
     2881 tattttccgc caccagcgcc cgcaattcaa aatcatcgac atcctgcatt gcttcgtttt
     2941 cttccaggca atgccagcgc atatcgagaa gtgcggcgct aagcagttcg ctcatctcat
     3001 accctttgtt gaacaggctg gcattacgca ttttctgttg cagttcgtca ggcattgctg
     3061 ccccgctctg ataatgccgg gcgtagcgag cgaatacctg cggatgcgtt gcccagtgtt
     3121 cgttgatttg cgacggaaat tcgacaaaat cacgcggcgt gttggtgccg gaaagcgtgg
     3181 cataacgctg gcgggcaaaa aggccgtgca gcgtatgacc aaattcatgg aataaggtta
     3241 ttacatcatc ccagagtaac aacgcaggct caccggcagc gggtttctga taattgcaga
     3301 cgttataaat taccggatgc gtttcattaa gcgttgattg ctcaacaaaa ttgcccatcc
     3361 atgcaccgcc gctttttgaa tcacgggcga agaaatcacc gtaaaataac gccagcccca
     3421 cgccattatg atcaaaaatt tcccacaccc gaacgtcagg atggtagaca ggaatatcaa
     3481 aacgttcgac aaacttaata ccgaagagct gattcgcggt ccagaataca ccttcattca
     3541 acaccgtgtt taattcaaaa tatggcttga gctgcgcttc atcaagatca aatttctccc
     3601 gccgtacctg ttccgcataa aatgcccagt cccacggctg cgcgctaaac ccgccctgct
     3661 gcttatcgat aaccgcctgt atggaggcta attcatcgct cgcacgttga cgcgccgctg
     3721 gaacaatttc ccgcataaag ttgagtgctg cttctggcgt ttttgccatc tgatcggcga
     3781 ttttccatgc ggcataatga ggaaaaccaa gcagtttcgc ctgctgtgcg cgaatctcta
     3841 ccagacgttg aatgatagcg cgggtatcat tgccatcatt tttttccgct cgcgtccagc
     3901 ccgcaataaa cagtttttca cgcgtcgcac gatcgcgcat ttcggcaagc gccggttgtt
     3961 gggtggtatt cagcagcgga atcagccatt tgttatccag acctttctcg cgagccgcct
     4021 ctgccgccag cgcaatctct tgctcactca ttcctgccag ctgcgcgata tcgttcacaa
     4081 ccagaccgcc ggatttattt gctgccagta atcgctggtt aaactggctg gtcagggtcg
     4141 cagcttctgt attcagtact tttaattttg ctttatcagc ttgcgcaagt ttggctccgg
     4201 caaggacaaa acgttgatga atcacctcca ccaggcggat ggattcacta tcaagcccca
     4261 gggattcacg gcgctgccag acagcatcta cccgcgcgaa taattcaccg ttcagataga
     4321 tatcattagc cagttccgcc agttcagcgg aaaactgttc atcaagacgc tgtaattcat
     4381 cattggtatg cgccgcagtc atcgcaaaaa agacgctggt aacgcgggta agtaattctc
     4441 cgctttgttc cagtgccaga atagtattgt tgaaatcagg cgtttgcggg ttaagcgcga
     4501 tggcagcaat ttctgcccgc ttttgctgca ttccctcatc gaatgccggg cgatagtgat
     4561 gattggcaat ttgatcaaaa tggggagcca gatacggtag tgtgctttgc acaaggaaag
     4621 gattcattgt tgtcattttc ttctcctgaa cgcgagatgt tccatagcgt aggcttactg
     4681 ataacggagt gcaatcttgc aaaccagtat tacccgctct taagcatcac gtgctatgtt
     4741 attgacacac aaaagcgttg aggaacagtg agatgatcgt tttagtaact ggagcaacgg
     4801 caggttttgg tgaatgcatt actcgtcgtt ttattcaaca agggcataaa gttatcgcca
     4861 ctggccgtcg ccaggagcgg ttgcaggagt taaaagacga actgggagat aatctgtata
     4921 tcgcccaact ggacgttcgc aaccgcgccg ctattgaaga gatgctggca tcgcttcctg
     4981 ccgagtggtg caatattgat atcctggtaa ataatgccgg cctggcgttg ggcatggagc
     5041 ctgcgcataa agccagcgtt gaagactggg aaacgatgat tgataccaac aacaaaggcc
     5101 tggtatatat gacgcgcgcc gtcttaccgg gtatggttga acgtaatcat ggtcatatta
     5161 ttaacattgg ctcaacggca ggtagctggc cgtatgccgg tggtaacgtt tacggtgcga
     5221 cgaaagcgtt tgttcgtcag tttagcctga atctgcgtac ggacctgcat ggtacggcgg
     5281 tgcgcgtcac cgacatcgaa ccgggtctgg tgggtggcac cgagttttcc aatgtccgct
     5341 ttaaaggcga tgacggtaaa gcggaaaaaa cctatcaaaa taccgttgca ttgacgccag
     5401 aagatgtcag cgaagccgtc tggtgggtgt caacgctgcc tgctcacgtc aatatcaata
     5461 ccctggaaat gatgccggtt acccaaagct atgccggact gaatgtccac cgtcagtaat
     5521 ttttataccc ggcgtaactg ccgggttatt gcttgtcaca aaaaagtggt agactcatgc
     5581 agttaactca ctcacaagca agaacgaatg accgtcgaaa cgcaacttaa tcccacacag
     5641 cctgtcaatc agcaaattta tcgtattctt cgtcgcgaca ttgtccattg cctgattgca
     5701 ccaggcacac cgttgtcgga aaaagaagtt tctgttcgtt tcaatgtgtc acgccagccg
     5761 gttcgtgaag cctttattaa actggcggaa aacggcctga ttcaaattcg tccgcagcgt
     5821 ggcagctacg tcaacaaaat ttccatggcc caggtgcgca atggcagttt tatccgtcag
     5881 gccattgagt gcgcggtggc gcgtcgggcg gcgagcatga ttaccgaaag ccagtgctac
     5941 caactggaac aaaatcttca ccagcaacgc attgccattg agcgcaagca actggatgat
     6001 ttttttgaac ttgatgacaa cttccatcaa ctcctgacgc agattgccga ctgtcaactg
     6061 gcgtgggata ccattgagaa cctgaaagcg accgttgatc gcgtgcgcta tatgagtttc
     6121 gaccacgttt ctccaccaga aatgctgtta cgccagcatc ttgatatttt ctctgccctg
     6181 caaaaacgtg atggcgatgc ggtagaacgt gcaatgacgc aacatttgca ggaaatcagc
     6241 gaatccgtgc gccagatccg ccaggaaaat agcgactggt ttagcgaaga gtaattcatt
     6301 tcctctcatc ccatccgggg tgagagtctt ttcccccgcc ttatggctca tgcatgtatc
     6361 aaaaaagatg tgagcttgat caaaaacaaa aaatatttca ctcgacagga gtatttatat
     6421 tgcgcccgtt acgtgggctt cgactgtaaa tcagaaagga gaaaatacct atgacgacct
     6481 acgatcgtaa ccgtaacgca atcaccactg gcagccgtgt tatggttagc ggcaccggtc
     6541 acactggcaa gatcctgtcg attgatactg aaggtctgac cgctgagcaa atccgccgcg
     6601 gaaaaaccgt agttgttgaa ggttgtgaag agaaactggc acctctggac ctgattcgtc
     6661 tcggcatgaa ctaagcgtgt gaatgccgcc gatggcggca ttgctttttt tacttcacgg
     6721 aatatttcgc cacggtcgct ttcgcgccat gcgctaataa agacaagtac gcttccgtca
     6781 cttttgcagt aaacaaacta ttgtctggca aatcatcacc aaagatcgcc ttaatcgcca
     6841 gcaatgactg gacgcgcgct ttcccttcag cactactttg tacagccttc tgaataacag
     6901 gtaacagtgg gtcactgatt tcaatcggat ttccctgttc atcaacacca ccgacataac
     6961 gcatccaacc cgcgacgccc agtgccagca gatcgaactt gctgtcatgc gccagatgcc
     7021 agcgaacaga atccaacatc cgctgtggca atttctggct gccatccatc gcaatctgcc
     7081 aggttcgatg acgtaacgcc gggttgctat agcgttcaat taatcggtta gcgtaatctt
     7141 gcaaatcaac gccctgcact ttcagcgtcg gcgcttgttc ctgcaacatc aagccatacg
     7201 ccgcataacg ataatgttca tcttccatgc agtcattaat gtgctgatat ccagcaagat
     7261 aacccaaata cgccaggaat gaatgactgc cgttgagcat gcgcaacttc atctcttcat
     7321 aaggcagcac atcactaacc agttcggctc ccgctttttc ccattctgga cgtccggcaa
     7381 caaagttatc ttctattacc cactggcgga aaggttcaca ggcaacgccc gcaggatcgc
     7441 ccacaccggt aagttgttcg attttcgcca gcgtatcctc tgtcactgcg ggcacaatac
     7501 ggtccaccat tgttgatggg aaagtcacgt tatcttcgat ccattgtgcc agttttacat
     7561 caacagcttt cgcgtatgaa gtgacaacgt cacgcataac atgaccgttt tctggcatgt
     7621 tgtcacatga catgacggta aatgcgggaa gtcctgccgc tttacggcgg gccagcgcct
     7681 caacaatcac ccctgttgct gttttcggct ggtggggatt ttgcacgtcg gcagcgacca
     7741 tcgggtgatc gagcattaac tgtccggtcg ccggagagtg gaaataccct ttttcggtga
     7801 ttgtcagaga gacaatcgcg atttgcggtt cacacatcgc agccaacacg gtttctaagc
     7861 catccatctg tacgtgcaag gctttcttaa cgacgccaac gacgcgagcc gtccacgcat
     7921 cggccgacat ttccgcaacg gtataaagat tatcttgctg ttgtaaatcg gcaatttgct
     7981 gttcgccgcc gattaagttg acctcataat atccccagtc actgaaatgt tctgtagcaa
     8041 gaatatcggc atacacgccc tgatgcgcac ggtgaaatgc accaaagcct aaatgaataa
     8101 ttcttggggc caggttatta cgatcataaa cagggagtgt cgcttttgct gataacaaat
     8161 tatttcccat aacaattcct taaatataaa tatggcaaac tatatgtttt gtta
//
LOCUS       DAZPAC010000183         8197 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZPAC010000183 DAZPAC010000000
VERSION     DAZPAC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8197
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.183"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(359..1555)
                     /gene="hemY"
                     /locus_tag="ACHUPM_003399"
     CDS             complement(359..1555)
                     /gene="hemY"
                     /locus_tag="ACHUPM_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR0871088.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1558..2781)
                     /gene="hemX"
                     /locus_tag="ACHUPM_003400"
     CDS             complement(1558..2781)
                     /gene="hemX"
                     /locus_tag="ACHUPM_003400"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR0871089.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPQGDTPAAAPQGE"
     gene            complement(2803..3543)
                     /gene="hemD"
                     /locus_tag="ACHUPM_003401"
     CDS             complement(2803..3543)
                     /gene="hemD"
                     /locus_tag="ACHUPM_003401"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR0871090.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            complement(3540..4481)
                     /gene="hemC"
                     /locus_tag="ACHUPM_003402"
     CDS             complement(3540..4481)
                     /gene="hemC"
                     /locus_tag="ACHUPM_003402"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR0871091.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4868..7414
                     /gene="cyaA"
                     /locus_tag="ACHUPM_003403"
     CDS             4868..7414
                     /gene="cyaA"
                     /locus_tag="ACHUPM_003403"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR0871092.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7454..7774)
                     /gene="cyaY"
                     /locus_tag="ACHUPM_003404"
     CDS             complement(7454..7774)
                     /gene="cyaY"
                     /locus_tag="ACHUPM_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR0871093.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7936..8078)
                     /locus_tag="ACHUPM_003405"
                     /pseudo
     CDS             complement(7936..8078)
                     /locus_tag="ACHUPM_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
BASE COUNT         1822 a         2170 c         2137 g         2068 t
ORIGIN      
        1 agctcaggtt aaatattcac tcaggaagca aagaggatta cagaattatc tcataacaag
       61 tgttaaggga tgttatttcc cgattctctg tggcataata aacgggtaga tgctcattcc
      121 atctcttatg ttcgccttag tgcctcataa actccggaat gacgcagagc cgtttacggt
      181 gcttatcgtc cactgacaga tgtcgcttat gcctcatcag acaccatgga cacaacgttg
      241 agtgaagcac ccactgttgt catacagacc tgttttaacg cctgctccgt aataagagca
      301 ggcgtttttt tatgcatcag aaaggtcccg gaggtgcttg cctccgggtg agaaggaact
      361 actgtggtgg gttgttctgc aacgttaaca tcaaaccgtc gcgacgcatc gctgcagctt
      421 cttccggctt gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg taggcgtccg
      481 gacgttgttt cagcgctgcg cggaaggcga gcgatgcttc ctgccattct ccgtgcttca
      541 tcagtgactg gcccagtgtg ctccacaaca gcgggcgatc gccgacgttt ttgatctgct
      601 ggcgcagcac tttttccagc tgttccggat tgttggtttt cagtcgagga atcggcagca
      661 gtaggcgatc gtcgtactgg cgtttcaggc catcgatgat aatttgctgg gcagtatcat
      721 gatcgtcaca ttcaataaga tgttccgcca ttgccacctg caacgctacc tgatgacgcg
      781 ttttccggct ttggtttttc caccagttac gcaaaccttc actaccgtta tcggcacgcg
      841 cctgatccat caggccaatc catgcctgtt gttccagcat tgcacgatgt tcttcatcac
      901 caacatgggc tttcgccatt gatgggatga tatccagcag agaactccat gcacctgtgc
      961 ggatatacgc ctgttccgcc agacgtaata cttccggatg gcgtggcgta acttccagca
     1021 gcttatccac gccgtggcgt gcagcatggt tttcattacg ggccagttgc agacgtacac
     1081 gggtgatttc taccggaatg gtgtcattgc cggccagctc tgctgcgcgt tccagatgtt
     1141 ggttggcgcg tgcttcatca ccacgttgtt gcgcggcttc ggcagccagt agatagttca
     1201 ccaccggttg ttccgcgtga tcggcatttt tcgccatcag cttttcaact tgctgataat
     1261 cgccttccgc cagtttcagc agcgcctgtt cggtctgctt acgggcacgg cgacgcttac
     1321 gtccgacaaa ccacccacgg gtgtgtgcgc ctgtgcggaa gatccgccgc agtagccact
     1381 caatggcaaa cagcactacc atcgccagaa tcaaaataat cgccaggccc gtgacgctgg
     1441 tttcgatatt gtagttgtca gtctggatca gcacgtaacc ctgatgaccg gcaatcatcg
     1501 ggccaaccac tatccccgca atcagcaaaa caaagagtaa taacactttt agcatggtta
     1561 ttctccttgc ggcgcggctg ccggagtatc accttgcggt gcaggtgcag gtgcaggtgc
     1621 aggtgcaggt gcaggtgcag gtgcaggtgc aggtttagct tccgttgtcc ccgctgccgg
     1681 ttgtgccagc aggttacgca cgcgggtctg catcagtttt tccagcatcg cctggctttg
     1741 cagggtttcc ggaagatcca tcgagatatt ttgctggctt aactggtcca cctcgtcgag
     1801 gaacgctttg gtggtggcat catcagtatc gtagtaagca cgtacccagg tggagacgtt
     1861 ctccagcgcc tggcgataag tctcttcctg atgacgcggt acagcttgtg ctgcgaccag
     1921 caggcgagag cgaatatttt cgcgcagata gatatcctga tttggcgcta acagcggtac
     1981 ggcggtgtca tcacgacggc gaatcgtaat gaagttgtcc ataaagttct gccagctttt
     2041 ttgcagattg atacgccatt cgctgatgga actggaaagc tcttcaccgt ctgaatccat
     2101 cggcgaacca tcgctgtcat tatcggccag acgcaggtta tctacctgat ttgaaagctg
     2161 attaagctta aggatgatgc cgtcataatc cacctgcgat actgcagaaa ggctggcgat
     2221 atcatcggta attgcccgac gaacggtaat cagactcggg tcattcatat ccgccaggct
     2281 ggcgtctgca cttttcagca acgctgcagc ggtcgtgacg tcctgatcgc tccacagctt
     2341 ccgtccggcg agtttcacca gaaaatcggc ctgggccagc agccaggttt tagcatcgct
     2401 gccggaaatg gtggcgacct tttgctggac ttcatccagc tgttttgcca gcgtttcttg
     2461 ctgacgattc gcctgttcaa gctgcgtagc ctgttgctta ataatgcctt ccagttcggc
     2521 tttttggctc tcctgggctt tttgcaatgc cgtcagttgg ttagccaggg catcgctggt
     2581 ggcggtctga ttgacggcct gttgtttacc ccagccatac aaaccgacgc ccgccgccag
     2641 agcaatagcg atagccgccg cgctgagaat caatgcggta ttgttcttac tctttttttc
     2701 tgttgcgaca ggttgtgacg tggtgtccac ggcctcccta gtctcttcaa ccacggcgga
     2761 ggttttttct tgttccgtca ttatggcttc ctgttatgag agttattgta atgcccgtaa
     2821 aagcgcatcg ttggcagcgt tatcggcgac cttaatgtct tgccagccca gttcccgggc
     2881 gagtttcgcc aaacgctcac tgacgaccaa tagtcgacag tgtagtaacc agtgctcacg
     2941 ataccattgt gggatcagcg accagagttg ctgcaacatt tcaccgctgg taacaacgac
     3001 cgtcgtcacc tcgcgggatt gccagcgcat cgcttcttct gcaccatcgt aatggattgc
     3061 gcatcgttga taacattcac aaaaagtgac ctcagcaccg cgcgccgtca gggtatcccc
     3121 aattagctca cgaccgccat tgccacgtaa tatcagcgca cgtttgcccg caatattttg
     3181 taattcaggt aattgtagca agacttcgct gatttcccga tcctgcgggt agagaatctt
     3241 ctgtccgctt acggtatgta gtgccagtgc ggtggtgcgt ccaatggcga aataattagg
     3301 tagtcggggc catttacgat cttgctgatg tagctgtgat tgggcaaaag caaccgcgtg
     3361 ttgcgagagg gcaaacaaca gatcgctctc ccccagcgct gccagttgat cagcaagttg
     3421 cggtaattgt tgacccggag aaaactcaat cagcggaaaa tgccaggcca cctgccccag
     3481 tgtgcgcaga cggctcacta actcttctcc agcgggagac gggcgggtga caaggatact
     3541 catgccgggg cgtctccgtt atagacttca gcgaggatct cgcgcgcgcc gttattcagt
     3601 agctcttctg ccagcgaaat ccccatttgt ttggcatttt gcggcgcacc gcggcgttca
     3661 ccgcgaataa tctgcgaacc gtccggcgcg ccgaccagcg cacgcagcca gatttcgcca
     3721 tcaataagct cggcgtagct accaattggc acctgacatc cgccttcgag acgggtattc
     3781 atggcgcgtt ctgcggtaac gcgcagtgca gtttcgtggt gattcagcgc ggcaagcagc
     3841 tcgcgagtgc gcgtatcatc aaggcggcat tcaataccga ctgcaccttg tcctaccgcc
     3901 ggaagagaaa tctcgggtgg taacgcggcg cgaatgcggg actccagacc taaacgtttt
     3961 agtccggcta cggcaagaat gatggcatcg tattcgccgt tatctagttt gctcaggcga
     4021 gtgccgacgt tgccgcgcag ggagcggata atcagatccg ggcggcgttc agccagttgg
     4081 cactggcgac gtaaactgga cgtcccgacg atactgcctg ctggtaacgc atccagactg
     4141 tcatagttat tggacacaaa ggcatcgcga ggatcttcac gctcacaaat agtgaccagt
     4201 cccagacctt gcgggaattc aaccggcaca tctttcattg aatgtacggc gatatcggcg
     4261 cgattttcga ggagcgcgac ttccagctct tttacaaata agccttttcc gcctactttc
     4321 gccagcggcg tatcaagaat cacatcgccg cgcgtcacca tcggtaccag ttcaacgacc
     4381 aggcccggat ggctcgccat caacttgtct ttgacatagt gtgcctgcca gagtgcaagt
     4441 gggctttggc gtgtggcaat tcttaaaaca ttgtctaaca tgcttgttac cgtcattatc
     4501 atccgtggtc catcctaaca tccttgccag agtgatgtca gtgttgtggt gaaacgtaga
     4561 cgcctgcgca aaccgtaaaa tgaggtctgg cagtggatcc tgacagacgt ttcacgctgt
     4621 cgtaataagg aatttacaga gaataaacgg tgctacactt gtatgtagcg catctttctt
     4681 tacggtcaat cagcaaggtg ttaaattgat cacgttttag accatttttt cgtcgtgaaa
     4741 ctaaaaaaac caggcgcgaa aagtggtaac ggttaccttt gacatacgaa atatcccgaa
     4801 tgccgcgtgt taccgttgat gttggcggaa tcacagtcat gacgggtagc aaatcaggcg
     4861 atacgtcttg tacctctata ttgagactct gaaacagaga ctggatgcca taaatcaatt
     4921 gcgtgtggat cgcgcgcttg ctgctatggg gcctgcattc caacaggtct acagtctact
     4981 gccgacattg ttgcactatc accatccgct aatgccgggt taccttgatg gtaacgttcc
     5041 caaaggcatt tgcctttaca cgcctgatga aactcaacgc cactacctga acgagcttga
     5101 actgtatcgt ggaatgtcag tacaggatcc accgaaaggt gagcttccaa ttactggtgt
     5161 atacaccatg ggcagcacct cgtccgtagg gcaaagttgt tcctctgacc tggatatctg
     5221 ggtctgtcat caatcctggc tcgatagcga agagcgccag ttactacaac gtaaatgcag
     5281 cctgctggaa agctgggccg cctcgctggg tgtggaagtc agcttcttcc tgattgatga
     5341 aaaccgcttt cgtcataatg aaagcggcag cctggggggc gaagattgtg gctccaccca
     5401 gcatatactg ctgcttgacg aattttatcg taccgccgtg cgtctcgccg gtaagcgtat
     5461 tctgtggaat atggtgccgt gcgacgaaga agagcattac gacgactatg tgatgacgct
     5521 ttacgcgcag ggcgtgctga cgccaaatga atggctggat ctcggtggct taagctcgct
     5581 ttctgctgaa gagtactttg gtgccagcct ttggcagctc tacaagagta tcgattcccc
     5641 atacaaagcg gtgctgaaaa cactgctgct ggaagcctat tcctgggaat acccgaaccc
     5701 acgtctgctg gcgaaagata tcaaacagcg tttgcacgac ggcgagattg tatcgtttgg
     5761 cctcgatcca tactgcatga tgctggagcg tgttactgaa tacctgacgg cgattgaaga
     5821 tttcacccgt ctggatttag tacgtcgctg cttctattta aaagtgtgcg aaaaactcag
     5881 ccgtgaacgc gcctgtgtag gctggcgtcg cgcagtgttg agccagttag tgagcgagtg
     5941 gggttgggac gaagctcgtc tggcaatgct cgataaccgt gctaactgga agattgatca
     6001 ggtgcgtgag gcgcacaacg agttgctcga cgcgatgatg cagagctacc gtaatctgat
     6061 ccgctttgcg cgtcgcaata accttagcgt ctccgccagt ccgcaggata tcggcgtgct
     6121 gacgcgtaag ctgtatgccg cgtttgaagc attaccaggt aaagtgacgc tggtaaaccc
     6181 gcagatttca cccgatctct cggaaccgaa tctgaccttt atttatgtgc cgccgggccg
     6241 ggctaaccgt tcaggttggt atctgtataa ccgcgcgcca aatattgagt cgatcatcag
     6301 ccatcagccg ctggaatata accgttacct gaataaactg gtggcgtggg catggtttaa
     6361 cggtctgctg acctcgcgca cccgtttgta tattaaaggt aacggcattg tcgatttgcc
     6421 taagttgcag gagatggtcg ccgacgtgtc gcaccatttc ccgctgcgct tacctgcacc
     6481 gacaccgaag gcgctctaca gcccgtgtga gatccgccat ctggcgatta tcgttaacct
     6541 ggaatatgac ccgacagcgg cgttccgcaa tcaggtggtg cattttgatt tccgtaagct
     6601 ggatgtcttc agctttggcg agaatcaaaa ttgcctggta ggtagcgttg acctgctgta
     6661 ccgcaactcg tggaacgaag tgcgtacgct gcacttcaac ggcgagcaat cgatgatcga
     6721 agccctgaaa actattctcg gcaaaatgca tcaggacgcc gcaccgccag atagcgtgga
     6781 agtcttctgt tatagccagc atctgcgcgg gttaatccgt actcgcgtgc agcaactggt
     6841 ttctgagtgt attgaactgc gcctttccag cacccgccag gaaaccgggc gtttcaaggc
     6901 gctgcgcgtt tctggtcaaa cctggggctt gttcttcgaa cgcctgaatg tatcggtaca
     6961 gaagctggag aacgccatcg agttttatgg cgctatttcg cataacaaac tgcacggcct
     7021 gtcagtgcag gttgaaacca atcacgtcaa attaccggcg gtggtggacg gctttgctag
     7081 tgaagggatc atccagttct tcttcgaaga aacgcaagac gagaatggct ttaatatcta
     7141 cattcttgac gaaagcaacc gggttgaggt gtatcaccac tgcgaaggca gcaaagagga
     7201 actggtgcgt gacgtcagtc gcttctactc gtcatcgcac gaccgcttca cctacggctc
     7261 aagcttcatc aacttcaacc tgccgcagtt ctatcagatt gtgaaggttg atggtcgtga
     7321 acaggtgatt ccgttccgca caaaatctat cggtaacatg ccgcctgcca atcaggatca
     7381 cgatacgccg ctattacagc aatatttttc gtgatgaacg tgccggaaag cgaggcttat
     7441 ccggcatgca atcttagcgg aaactgactg tttcacccgc ctgctgcgtc gccgcctgtt
     7501 ccagcaaatc ccagaaggtt tcgccgctgc gatcgcaaat ccactcatcg cctttcagat
     7561 caaaatggta gccgccttgt ttggttgcca gccatacctg gtgcagcggt tcctggcggt
     7621 tgataatgat tttgctgccg ttttcaaagg taatggtcag cacgccgccg ttgatttcgc
     7681 agtcgatatc gctgtcgcca tcccagtcgt ccaggcgttc ttcaatggtc agccagagtt
     7741 gatcagccag gcgatgaaat tcactgtcgt tcattgttgt atcctgtttt taagtgatgg
     7801 cggcagtata gcggcatggg gtcagggctt caaagtttgc acctctgcgg ctgcgttccg
     7861 gcacgattca tccgtcaccg gaataatgat gtctctgtgt agcgaaagat ttgtctcttc
     7921 attagggcgc agttacacca cgcctttccc tgtttctggt aaacattatg atcaggttta
     7981 ccgagcgagc atcctcacgc tgacggaact aaaaaagaca acaaacaaac cacattgcga
     8041 tagtgcatag agccatcctg gcgcgaggtg ccgatcacga aactaccagc aaaacataaa
     8101 tccccacgag taagcgttat actcgcagca tttcctcact tttcagactt cataaagagt
     8161 cgctaaacgc ttgcttttac gtcttctcct gcgatga
//
LOCUS       DAZPAC010000184         8182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZPAC010000184 DAZPAC010000000
VERSION     DAZPAC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.184"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHUPM_003406"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHUPM_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFR0871094.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHUPM_003407"
     CDS             complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHUPM_003407"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR0871095.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHUPM_003408"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHUPM_003408"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR0871096.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYGTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="ACHUPM_003409"
     CDS             6679..>8182
                     /locus_tag="ACHUPM_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFR0871097.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINSQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1978 a         2029 c         2322 g         1853 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact tggcggttta tggcacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 tcacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAZPAC010000185         8156 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZPAC010000185 DAZPAC010000000
VERSION     DAZPAC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.185"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..450)
                     /locus_tag="ACHUPM_003410"
     CDS             complement(<1..450)
                     /locus_tag="ACHUPM_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR0871098.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNY"
     gene            complement(567..1613)
                     /gene="ansB"
                     /locus_tag="ACHUPM_003411"
     CDS             complement(567..1613)
                     /gene="ansB"
                     /locus_tag="ACHUPM_003411"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR0871099.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1789..2508)
                     /locus_tag="ACHUPM_003412"
     CDS             complement(1789..2508)
                     /locus_tag="ACHUPM_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR0871100.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2692..3018)
                     /locus_tag="ACHUPM_003413"
     CDS             complement(2692..3018)
                     /locus_tag="ACHUPM_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR0871101.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3018..3737)
                     /gene="trmB"
                     /locus_tag="ACHUPM_003414"
     CDS             complement(3018..3737)
                     /gene="trmB"
                     /locus_tag="ACHUPM_003414"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR0871102.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3898..4950
                     /gene="mutY"
                     /locus_tag="ACHUPM_003415"
     CDS             3898..4950
                     /gene="mutY"
                     /locus_tag="ACHUPM_003415"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR0871103.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4978..5253
                     /locus_tag="ACHUPM_003416"
     CDS             4978..5253
                     /locus_tag="ACHUPM_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR0871104.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5318..6397
                     /gene="mltC"
                     /locus_tag="ACHUPM_003417"
     CDS             5318..6397
                     /gene="mltC"
                     /locus_tag="ACHUPM_003417"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR0871105.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6599..7855
                     /gene="nupG"
                     /locus_tag="ACHUPM_003418"
     CDS             6599..7855
                     /gene="nupG"
                     /locus_tag="ACHUPM_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR0871106.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7904..>8156)
                     /locus_tag="ACHUPM_003419"
     CDS             complement(7904..>8156)
                     /locus_tag="ACHUPM_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR0871107.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1923 a         2159 c         1987 g         2087 t
ORIGIN      
        1 ataattgaaa tcttcgctat tcacgatcag atatttatta ttcgttttga tatcgtcgac
       61 cacccgccca cgatcgctgg ctgccgcctg aaaagagtaa aagttcaccg accagccaga
      121 cgcgggcgtt cctttactgg ttaacatcgc cgagaatttt accggtttat ctttcgtcca
      181 ggtctgttcg agcagttcgt aatctgccaa ctgaccgacc caggtataga gatcgtcact
      241 ggaagagaca tcgccttccg ctgaccaggt ggcctgatta cccttagcat caagattttt
      301 taaggtgata gcatcaagct tgagaatgcc gtgatactgc tttttgaatt gatcttttaa
      361 gatattttca gttggcggtt cgccatctgc ccaggcatta ccagtcatca acataagcag
      421 tgccgtgcca acaatccatt gctttttcac ccgaaaaacc tcacgcggaa taatttattc
      481 cggtgattat cattagggta atgaaaattg gcaatggacg taagtctgaa gtgaaaaagc
      541 cccggcacga taccggggcg aggtgattag tactgattga agatctgctg gatctgctgc
      601 ggatctttgg tttgcgtcag agccagctgc agcaggacgc gcgctttttg cgggttcagc
      661 gtgccagagg cgacgaagcc gtatttcgca tcatccactt cagcatcctg agtggtagca
      721 cccgtcggta cgcgggaaga acgcactact gcagtgccgt ttttcgcggc ggttgccagg
      781 gtgtcgaaca cggatttata caggttacca ttacccacac cagcgctaac gatgccatca
      841 tagcccgcat ctaccagtgc tttagccgga agatcggatg cgttagcgta gttataaacg
      901 atgccgactt tcggcagctc attcagctta gagacatcga atggcgtatc gctggtgtgc
      961 ttacgtgccg gggtacgttg gtagtcaatc ttaccgttgt gaatgtatcc cagaggaccg
     1021 tagttaacag acttgaaggt cgctacgtcg gtggtgttgg ttttggtgac atcgcgaccg
     1081 tccagtacgg tgtcgttcat caccaccagc acgccacgat tagcggaggc tttatcagct
     1141 gcggtcacta ccgcgttata caggttgaat ggaccgtctg cgctcatgga cgtggacggg
     1201 cgcattgcgc cgaccatcac caccggtttg tcgcatttca ccgtcaggtc gaggaagtaa
     1261 gcggtttctt ccatcgtgtc ggtaccgtgg gtaatgacga agccgtcggt tttatcgcag
     1321 tcggtgttaa tttttttcgc cagtgtcagc cagacattat cgttcatgtc ctgggagccg
     1381 atattcacta cctgctcgcc tttaacgttc gcaatgtctt ttagttgcgg caccgcatta
     1441 accagatttt ctacgccaac tttacccgct gtgtagttag atttggttgc ggagtcacca
     1501 ccaccggcaa tggtcccgcc ggttgctaaa atggtgatat tgggtaatgc caatgctgca
     1561 ccactaaaac ccataaccag tgcggcaagt gccgtctttt tgaaaaactc catttcattc
     1621 ctccagttac gtgaacgcta cgcattatcc cttagctctg tatgggaaat ttgacgttaa
     1681 acaatttaca acgtgaatat attttggaga tctacaaagt tagaggcagg taacaaaacg
     1741 aagaattaaa cggcataaaa aagtattatg ccgtcttaaa atagaggatt attttaaatt
     1801 accgaccagg gctttgcggc tatcttccag agtcacaacg cggctacaaa catctttgcc
     1861 aaactgctgg aaatcttttt cctgcttttt ccactctgtt tggattgagg attgcagccc
     1921 ccccaggctt cccagcacat tctgtaatgg gttaccgccg cttttcagca ccgctttcgc
     1981 gcccatttca ttaatgctgt cctgtaaaat tccacccatt gcctgattca ctaattgctg
     2041 gccttcggcg cgaacctgat caatggcttt atagtgaaac gtcaggccat cgctgcgcgt
     2101 ttcgataatg cggttcatct gctctttcag ctgcgcatca agtttggtca gacggctgcg
     2161 cattttgctg ctttcgccca tctcctgaac gataatttta tccagcgcaa tacgggcttt
     2221 ttcgacgcgg cttttcgcgc cttcatcaat ccacggcagc gtgctgcgta gttcagcctg
     2281 ataatccttc gcctgctcgc gctgggcggc attcagggaa tattgcttac cgttatacat
     2341 cacgttgccg tctggcgtga tcaccagatt gccgttttcg cccttcacct gcacggtttg
     2401 cgggctgaca atcacatcgt cacgcggcgt gacgctgcac tggtagtcgg cgtgagcggt
     2461 cattgccgtc actgaaagtg ctgccgccag cagcattttg cgcatcatag tcttccctca
     2521 agaaaaaatc aggccagcat ttgctggccc cagattgata acaaagtacg cgttttccat
     2581 gccggatgcg gcgtaaacac cttatcccgc ctactcgatc gtgcatcttt ccaggcctga
     2641 taagcgtagc gcatcaggca gttttgcatt tgtcatcgcc cgtatgcttt cttagtccca
     2701 ccaaacgtcg aaaagttcgc tggtgcgtac ctcatccagt ttgcgctctt ccagccactt
     2761 acgcacaatc gcctgatgtt cttcggtgca tttgccgatt tcctgcatgc agatcagacc
     2821 ttcccaggcc agataaccgc tgccgtcaaa ggccagtttg ttcggttcga taacctcgtt
     2881 aataaaatca tcaacggttt tatcaatctg ttcttccgat gtaccttccg ggaatcgcca
     2941 tgccaccgaa aatcctaatt cctggaattc gtcgatgtgc atttttttac gcagacgacg
     3001 gctacggttc tttgccatta tttcaccctc tcgaacatta agtcccatac tccgtgacca
     3061 agacgatgac cacgttgttc aaatttcgtc accggacgtg atgccggacg cggcacgtaa
     3121 tcattgctct ctgacaggtt tttataaccg tcaatagaag acatcacttc aagcatatgt
     3181 tccgcataag gttcccagtc ggtcgccata tggaatacac cccccagctg cagtttgctt
     3241 tttaccagtt cggcaaacgg cacctgtacg atacggcgtt tattatggcg cgctttgtgc
     3301 cacgggtcag ggaaaaagag ctgcaccatg cgcaatgaat tgtcaggaat cattttatgc
     3361 agcacttcaa ccgcatcgtg acacatcacg cgcaggttgc ttaaaccttc ttcatgcgca
     3421 gaagccaggc acgcaccaac gcccggtgaa tgcacttcaa tgccgaggaa gtcctgctca
     3481 gggcgatctt tagccattgc caccagcgac gcccccatgc caaaaccaat ctcaagcgtc
     3541 accggcgctt cacggccaaa aagcgcgggg aaatccagca tatcttcgct gaactcaacg
     3601 cccatcaccg gccagtagtt ttccagcgca tgttcctggc ctttggtcag tcgcccctgg
     3661 cggcgcacaa aactacggat acggcgcagt gggcggccgt tttcatcaaa ttccggtgaa
     3721 atgacgtcgt ttttcataaa gatttagtcg cttgtgaaag tgttctgaaa acgggcatta
     3781 tccaaagtta gttgccggat gcaagcatga taaggccgtg gctgcggaaa gttccggttt
     3841 acaccctgcc gtcgctgtgc tgcaatcttg cccccaacaa cagtgaattc ggtgaccatg
     3901 caagcgtcgc aattttcagc ccaggttctg gactggtacg ataaatacgg gcgaaaaacg
     3961 ctgccctggc aaattgacaa gacgccctac aaagtatggc tctcagaagt gatgttgcaa
     4021 caaactcagg ttgcgaccgt tatcccctat tttgaacgct ttatggcgcg cttcccgacg
     4081 gtgaccgatc tcgccaatgc gccgctcgac gaagttctcc acttgtggac cgggcttggc
     4141 tattacgccc gcgcgcgcaa tctgcataaa gcggcacaac aagtggcgac cttacacggc
     4201 ggtaaattcc cggaaacctt tgaagaagtc gcggcgttac cgggcgtcgg gcgttccacc
     4261 gcaggcgcga ttctctcgct ttctctgggt aagcactttc cgattctcga cggtaacgtc
     4321 aaacgcgtgc tggcgcgctg ctatgctgta agcggctggc ctgggaaaaa agaggtcgag
     4381 aataaattat ggagtttgag cgagcaggtg acgcccgcgg tcggcgtgga acggtttaat
     4441 caggcgatga tggatttggg cgcgatgatt tgtacgcgct cgaagccgaa atgttcgctc
     4501 tgtccgctac aaaacggatg tattgccgcc gccaataata gctggtcgct ttatccgggc
     4561 aaaaaaacga aacagacgct gccggagcgc accggctact ttttgctgtt acagcacgaa
     4621 gatgaagtat tgctggcgca gcgtccgccg agcggattgt ggggcggttt atactgtttc
     4681 ccgcagtttg ccgacgaaga aagtttgcgg cagtggctgg cgcaacggca gattgctgcc
     4741 gataacctga cgcaactgac cgcgtttcgg cataccttca gccatttcca cttagatatt
     4801 gtgcctatgt ggcttcccgt gtcgtcattc accggctgca tggatgaagg caatgcgctc
     4861 tggtataact tagcgcaacc gccgtcagtt ggcctggcgg ctcccgtgga gcgtttgtta
     4921 cagcagttac gcactggcgc gccggtttag cgcgtgagtc gataaagagg atgatttatg
     4981 agcagaacga ttttttgtac tttcctgcaa cgtgaagcag aaggtcagga ttttcagctg
     5041 taccccggcg agctgggaaa acgcatctat aacgagatct ccaaagaagc ctgggcgcag
     5101 tggcagcaca agcaaaccat gctgattaat gaaaagaaac tcaacatgat gaatgccgag
     5161 caccgcaagc tgcttgagca ggagatggtc aacttcctgt tcgagggtaa agaggtgcat
     5221 atcgagggct atacgccgga agataaaaaa taaaaacagt gccggagcac gcctccggca
     5281 acttgcataa aaacaaacac aacacgcacc cggaatgatg aaaaaatatc tcgcgctggc
     5341 tttgattgcg ccgttgctca tctcctgttc gacgaccaaa aaaggcgata cctataacga
     5401 agcctgggtc aaagatacca acggttttga tattctgatg gggcaatttg cccacaatat
     5461 tgagaacatc tggggattca aagaggtggt gatcgccggt cctaaggact acgtgaaata
     5521 caccgatcaa tatcagaccc gcagccacat caacttcgat gacggtacga ttactatcga
     5581 aactatcgcc gggacagaac ctgccgcgca tttgcgccgg gcaattatca aaacgttgct
     5641 gatgggtgac gatccgagtt cggtcgatct ctattccgac gttgatgata ttacgatttc
     5701 gaaagaacct ttcctttacg gtcaggtggt ggacaacacc gggcagccga ttcgctggga
     5761 aggtcgcgca agcaacttcg cggattatct gctgaaaaac cgtctgcaaa gccgcagtaa
     5821 cgggctgcgt atcatctaca gcgtcaccat taacatggta ccgaaccacc ttgataaacg
     5881 tgcgcacaaa tatctcggca tggtccgcca ggcgtcacgg aaatatggcg ttgatgagtc
     5941 gctgattctg gcaattatgc agaccgaatc ttcctttaac ccgtatgcgg tcagccgttc
     6001 cgatgcgctg ggattaatgc aggtggtaca acatactgcc gggaaagatg tgttccgctc
     6061 gcaggggaaa tccggcacgc cgagccgcag tttcttgttt gatcctgcca gcaatattga
     6121 taccggcacc gcgtatctgg cgatgctgaa caatgtttat ctcgacggaa ttgataaccc
     6181 aacatcgcgg cgttatgccg tcatcaccgc ctataacggc ggcgcaggca gcgtgctgcg
     6241 agtcttttcg aatgataaga ttcaggctgc caatattatt aacaccatga cgccgggcga
     6301 tgtttatcag acgctgacga cccgccatcc ctctgcggaa tctcgccgtt atctttataa
     6361 agtgaatacc gcgcaaaaat cctaccgccg ccgataattc cattaaccgc ccctgacgat
     6421 gctcaggggc aaaaatgtta tccacatcac aatttcgttt tgcaaattgg gaatgtttgc
     6481 aattatttgc cacaggtaac aaaaaaccag tccgcgaagt tgatagaatc ccatcatctc
     6541 gcacggtcaa atgtgctttt tcaaacactc atccgcatca caatgtgagg aaattaacat
     6601 gaatcttaag ctgcagctga aaatcctctc ttttctgcag ttctgtctgt ggggaagttg
     6661 gctgacgacc ctcggctcct atatgtttgt taccctgaag tttgacggtg cttctattgg
     6721 cgcagtttat agctcactgg gtatcgccgc ggtatttatg cctgcgctgc tggggattgt
     6781 ggccgacaaa tggttaagtg cgaaatgggt atatgccatt tgccacacca ttggcgctat
     6841 cacgctgttc atggcggcac aggtcacgac gccggaagcg atgttccttg tgatattgat
     6901 taactcgttt gcttatatgc caacgcttgg gttaatcaac accatctctt actatcgcct
     6961 gcaaaatgcc gggatggata tcgttactga cttcccgcca atccgtatct ggggcaccat
     7021 cggctttatc atggcaatgt gggtggtgag cctgtctggc ttcgaattaa gccacatgca
     7081 gctgtatatt ggcgcagcac tttccgccat tctggttctg tttaccctga ctctgccgca
     7141 tattccggtt gctaaacagc aagcgaatca gagctggaca accctgctgg gcctcgatgc
     7201 attcgcgctg tttaaaaaca agcgtatggc aatcttcttc atcttctcaa tgctgctggg
     7261 cgcggaactg cagattacca acatgttcgg taataccttc ctgcacagct tcgacaaaga
     7321 tccgatgttt gccagcagct tcatcgtgca gcatgcgtca atcatcatgt cgatttcgca
     7381 gatctctgaa accctgttca ttctgaccat cccgttcttc ttaagccgct acggtattaa
     7441 gaacgtaatg atgatcagta ttgtggcgtg gatcctgcgt tttgcgctgt ttgcttacgg
     7501 cgacccgact ccgttcggta ctgtactgct ggtactgtcg atgatcgttt acggttgcgc
     7561 attcgacttc ttcaacatct ctggttcggt gtttgtcgaa aaagaagtta gcccggcaat
     7621 tcgcgccagt gcacaaggga tgttcctgat gatgactaac ggcttcggct gtatcctcgg
     7681 cggcatcgtg agcggtaaag ttgttgagat gtacacccaa aacggcatta ccgactggca
     7741 gaccgtatgg ttgattttcg ctggttattc cgtggttctg gccttcgcgt tcatggcgat
     7801 gttcaaatat aaacacgttc gtgtcccgac aggcacacag acggttagcc actaattacg
     7861 caaagaaaaa cgggtcgcca gaaggtgacc cgttttttta ttcttacttc aacacataac
     7921 cgtacaatcg tttcatgcca tccgcatcgg tttcgctata gacaccttgc aactccggcg
     7981 aaaatcccgg caacaaattc acgccttctt ccagcgcaag gaaataacgt tgaaccgccc
     8041 caccccagac ttccccggga accacgcaaa gcacgccagg tggataaggc aacgcccctt
     8101 ctgccgcaat tcgcccttcg gcatcacgaa tccgcaccaa ctccacttca ccgcga
//
LOCUS       DAZPAC010000186         8035 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZPAC010000186 DAZPAC010000000
VERSION     DAZPAC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.186"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1340
                     /gene="yjeM"
                     /locus_tag="ACHUPM_003420"
     CDS             <1..1340
                     /gene="yjeM"
                     /locus_tag="ACHUPM_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR0871108.1"
                     /translation="MMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWM
                     VSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIA
                     RITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVF
                     AIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLS
                     NGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTL
                     CYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAF
                     FNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVTF
                     ANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(1491..4814)
                     /gene="mscM"
                     /locus_tag="ACHUPM_003421"
     CDS             complement(1491..4814)
                     /gene="mscM"
                     /locus_tag="ACHUPM_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR0871109.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(4836..5804)
                     /gene="asd"
                     /locus_tag="ACHUPM_003422"
     CDS             complement(4836..5804)
                     /gene="asd"
                     /locus_tag="ACHUPM_003422"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR0871110.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5901..6953)
                     /gene="rsgA"
                     /locus_tag="ACHUPM_003423"
     CDS             complement(5901..6953)
                     /gene="rsgA"
                     /locus_tag="ACHUPM_003423"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR0871111.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7048..7593
                     /gene="orn"
                     /locus_tag="ACHUPM_003424"
     CDS             7048..7593
                     /gene="orn"
                     /locus_tag="ACHUPM_003424"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR0871112.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7804..7879
                     /locus_tag="ACHUPM_003425"
     tRNA            7804..7879
                     /locus_tag="ACHUPM_003425"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7837..7839,aa:Gly,seq:gcc)
BASE COUNT         1851 a         2080 c         2162 g         1942 t
ORIGIN      
        1 taatgatggc tgaaatggga gctgcttatc gcaaagaaga aggtggtatc tattcctgga
       61 tgaataatag tgtcggacca cgttttgcct tcattggtac gtttatgtgg ttttcctctt
      121 atatcatctg gatggtgagt acctccgcga aagtttgggt accgttctca acattcctct
      181 atggtagcga catgacccag cactggcgta ttgccggact ggagcctacg caggtggttg
      241 gtctgctggc agtagcatgg atgattctgg tcaccgtcgt tgcttcaaag gggattaata
      301 aaattgcccg cattactgcg gtgggcggta ttgcagtaat gtgtctgaat ttagtattgc
      361 tgttagtaag cattactatt ttgttattaa atggtgggca tttcgcgcag gatattaatt
      421 tccttgcatc accgaacccg ggttatcagt ccggtctggc aatgctatcg tttgtggtat
      481 ttgctatttt tgcctatggc ggaattgaag cggttggtgg tctggtcgat aaaacggaaa
      541 atccagaaaa gaactttgcc aaaggtattg tttttgccgc tattgttatt tcaatcggtt
      601 attcgctggc aatattttta tggggcgtca gcacaaactg gcagcaggta ttaagtaatg
      661 gttccgttaa ccttggcaat attacctatg tgctgatgaa gagcctcggg gtgacgctgg
      721 gtaacgcact gcatttgtca cctgaagcgt cattgtcgct gggcgtatgg tttgcgcgta
      781 ttaccggact ttcgatgttc ctcgcttata ccggtgcgtt ctttacgctt tgctattcac
      841 cgctgaaagc catcatccag gggacgccga aagcgttgtg gccggaaccg atgacgcgcc
      901 tgaatgcgat ggggatgccg tctatcgcca tgtggatgca gtgcgggttg gttactgtct
      961 tcatcctgct ggtttcgttt ggtggcggta ccgcatccgc gttctttaac aagctgacgc
     1021 tgatggcgaa cgtgtctatg acgcttcctt acctgttcct cgcgctggct ttcccattct
     1081 ttaaagcacg tcaggatctc gacagaccat ttgtgatttt caaaacgcgt atgtcggcaa
     1141 tgattgcgat ggtggttgtc gtactggtgg tgacatttgc gaacgtcttc accattattc
     1201 agcctgtggt tgaagccgga gactgggaca gcacattgtg gatgattggc ggccctgtct
     1261 tcttctcgct gttagcgatg gcgatttacc agaactattg cagtcgcatg gcgaataaac
     1321 ctgagttagc tctcgactga tcatatgaaa tcctgcaact catcgtctct ccggcgatga
     1381 gttgtcagga agtaatgcgt atggatgata ctctccaggg ccggtgataa ctgaagatga
     1441 tgttaagctg ctaaatgagc cacgttgaca tcatttggca gcttcatgca ttacaaactt
     1501 cccgcctgac gacctttgcc cgcagacgtc agcgttctcc ccgtttgttt accgttgagg
     1561 ctttccagac gcatctggaa gggcgggaat ggcatatcga taccgtgggc atggaagcca
     1621 gccagaatca gctggtggat ctcatggcgt agcggcatac ggtgacccat ctcagcggca
     1681 taaatacgca gctcgaaaat ctgaatccct tgttgcagat ccaccaggaa gacttccggt
     1741 gccgggttgt cgatcaccag cgagcagcga cgcgctgcgg tgagcaggat ttccgtcact
     1801 tcttcactgt tggcatcagc aggggccgga atcgtcaaca ctacgcgcgt gaccgagtca
     1861 gagagcgacc agttgataaa ctgctcggta ataaacgcct tgttcggcac gattatctct
     1921 ttacggtccc agtcgctgat ggtggtggcg cgggtgttaa ttttcgtcac gctaccggtg
     1981 agatcgcgaa ttgtcaccgt atcgccaatg cgaatcggtt tttcgaacag gataatcaga
     2041 ccagagataa agttggcgaa aatttcctgc aaaccaaaac cgagaccaac accgagcgcg
     2101 gcgaccagcc actgcaattt cgaccactca ataccaatca tcgagaagcc gaccagcccg
     2161 ccaatcagca tcagcagata tttggtgatg gtggtgatgg cgtagcccgt acccggcgtt
     2221 aaatccaggt gctgcaaaat cgccagttcc agcagcgcgg gcaggttgcg caccagctgc
     2281 gtggtgatga taaacaccag aatggcaatc agcaccgcac cgagggtaat tggctccaga
     2341 ctttctacgc cctgtaccgt ggaggtgaca tcccacagcg aaatattttc gaggaagccg
     2401 aaagcggaat ggatttctga ccacagcaca atgacagaaa gcagggcgat caacatcaaa
     2461 attgagcgca ccagccgcaa ggattgcgca ctgatggcat cgagatcgac ttcgctttca
     2521 tcgacttcaa ttgctccttc cgggctactg tgatgatgcg cttcctcttc gccacgcgca
     2581 cgttgcgcta acatctctgc ccggcgatgc ttcgcccggt caaacgccag cctgcggcgc
     2641 tggatcagca tccagcggcg gataacgtga taaaccacca gtagcaggaa ccagatggca
     2701 accgaggttt caagcctcgc taacagcgcc tgtgccgttg ccagataacc cacagccgac
     2761 gccagaatgg caaccaacgg cgcgccaatc atcatgttcc acagcatatg gttggtaatg
     2821 ttgtcgccgc tgccttcttt gttgagatac agcggaatcc cggccttttt caggctgagg
     2881 gtgaccaccg ccagcgcacc gcaaatgagg ataaagcaaa gccttcccag cgaaccggag
     2941 aattcacggt cgtcgaggtt atcgaacatc atcagcgcca taatcagcgg cacaataagc
     3001 ccgatgctca tcaggtagta gcgcatccca cgagatacgc gttcgcgcgg ccagccaaaa
     3061 tgagcgataa acaagccgtt cgggcgggca aaggtggcgc aaatcatcac tacccacagc
     3121 agcggcacgg tggccgttac accatcgcca atcgcgaccg ccagcggata aggccacgcc
     3181 tcgcgcaagc cgtaacccag cgtcatccac agcaccggta acggtgacgc gacgagaatc
     3241 gaccagaaaa gagtgcgcaa cgtcagccag aagtgatcct gagtcacttt gccaactttc
     3301 gccgccgaac gttcaagaaa acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc
     3361 agaatcaacg cgccaaacag cggcaaaatt gtctctttgc tggtcagcat catcacactg
     3421 gctttgccca actgactgaa ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt
     3481 tccagcggcc aggcgatggt catcggacgc acgtcagagg tccagaacag atagcggtgc
     3541 gttgcttcgt tcacctcttt cagcgcatcc tccagttgcc cgttggagac tttcagcttg
     3601 gtcagttcca atagtagcgt gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga
     3661 gtgcgcagtt gtgcttccag aatacggttt tgctcggcgg tcagcggctg accgtcggcc
     3721 tgatggattt gccgtagcag cggctgttta ttaagcagat cctcataacg taaccgttgc
     3781 acacgcaact gcgccatttc ggtatcaagc tgttgtggtt ttggcatttc tggtagccgt
     3841 gccacctgcg ctcgcaacgc ttcaccgagc agattggacg atcccagcca ttgcgactgt
     3901 tcacgcagcg tattcaacgc ctgccggacc tgtaacgtct ggctggcagc ctgacgctgt
     3961 tgcgaggcaa cgagatccat ccgctgcgcc tgttgattca gcgctgccga tagttcgcgg
     4021 ttaattttga attgcgcgac gatatctttc ggcaaatcgg cactgttttc tgccagtaat
     4081 tcggtacttt ccagcgcccg ctccgcctca agctgacgtt ggctattcaa ctgattacgt
     4141 aaggcctgca aatacgcatc cagttgctgg ctctcttttt ccgcaagctc tgagcgtaag
     4201 cgcgctaatt cttggcggtt attggcagac agctgcgcca gctccagttc atcaacgagc
     4261 gccttaagac gtgcagagtc agactgcaac gcgaaatttt gtgcctgatt gagcggagta
     4321 ttgccggtaa gcgttcccag gcggcgctcg atctcattta actgacggcg ggcgtcggtt
     4381 tgctgttgcg gcagttgatt cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc
     4441 tgggcctgac ggcttttatc cagcaactgg ctgctgacct ggagaatttc ctgattcagc
     4501 gcgtcggtag acattcccgg cgacacgctg cgcggcttgt cacgcatgtt gtttaattgt
     4561 gcgcgcagag tagcggagag tttcggataa ttatcgataa cttgctgata ttgtttgatg
     4621 cgctcaaggg aaccttttcg ttcctcaagc gcatttaagg cagactgaag cgcctctacg
     4681 acttccggct gtgcgggttt cgccgctttt gcctgctcca gttcctgagt gatttgtttg
     4741 ctatcggggg ccgtcgcggc gtacgccccc caactgaggc accaggccat cagaaaagtg
     4801 ataatcaggc gcacgtcagc gtttcctttg atggattaga cctggtcttt tttgtcgtca
     4861 accaatgggc tggcgtcgtg ttctgcttcg atctcttcag caggaagcgg ggcaggttca
     4921 gcgtctggcg taacaaaggt ttcggtagat actgccagcg gctggcaaat tttcgtgaca
     4981 gacaggcttt ccagttgctc aaccagattc actttacccg gtgcaaacag gttgataacg
     5041 gtggaaccga gtttaaagcg acccatttcc tggcctttca gcagtgccac agaaccgtcg
     5101 ttttccccgg caggccaggt ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg
     5161 cccgcccaga ccgtctcaat gctgccaaca atcgtcgctc cgaccagaat ctgcgccatt
     5221 gggccaaatt cggtatcgaa aaggcaaatc acgcgttcgt tacgggcaaa cagattcggc
     5281 acgttctgag ccgtgagatg gttaacggag aagagatcgc ccggcacgta gatcatctca
     5341 cgcagaatac cgttgcacgg catgtgtacg cggtggtagt cacgcgggga gaggtaagtg
     5401 gtcacaaacg taccgttgcg gaacaggtcc gccatcagat agttgcctgc cagcagggct
     5461 tcgaggctgt agttgtggcc tttggcttgc aggattttat cttcttcgat tttacccaac
     5521 tggctgataa cgccatcggc aggcatgacc agtacattcg gatcggtatc gattgggcgt
     5581 acttcgtcac gcagcggacg gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc
     5641 ggcttttgcg cctctttcat gtcgaccttg tagtatttaa cgaacagatc gataaccagt
     5701 tttgtcaacc atcctgcccg cttgcttgcg ccccaacccg ccaggcgagt aagccatagt
     5761 ttcggcagaa tgtactgtag cgaaagttta aatgaattta acaaggtagc ctccaggcca
     5821 ttgttttgtc gttcctgatt cggcctacat gccggatcct gaaaaaaagg ggacgattct
     5881 aacgacggtt agcttaattg tcagtcatcc gtatcagaaa agtttttacg cgtttttacc
     5941 tgcgccatgc tttccagaat acggtgatag ttttcgaaac gggtttccgc gattttccct
     6001 tcctcaaccg cttcccggat agcgcagccc ggatcggtat cgtgtttgca atcgcgatat
     6061 ttacacagac ctaaatagtc atggaattcg acaaagccct gagtgatttg ttccggctcc
     6121 aggtgccaga ggccgaactc acgcacacca ggggaatcaa tcacatcacc gccgtgcggg
     6181 aagtgataca gccgagcggc ggtcgtggtg tgctgaccaa gccccgagtt gtcagagaca
     6241 tcgttggtca ggatctcttt ttgcagcccc agcaacgcgt tcagcaggct ggatttgccg
     6301 acgccagact gcccggcaaa aatgctgatg cgcccggtca acgcctcttc cagcggtttt
     6361 agcccatcct gagtatggct ggaaaccatc aatacgcgat aaccgatatt gcggtagata
     6421 tccatctgct cgttgacgaa cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc
     6481 acaataatcg gctcaatctg caaggtttcg caggccacta ggtaacggtc gataatattg
     6541 agcgacagct ccggcaaaat ggcggagaca atgacaatct ggtcgatgtt ggcggcaata
     6601 ggttttacgc cgtcgtagaa atccggacgc gtcaacaccg aggtacgctc atgcaccgct
     6661 tccacgatcc ctttgacatt tacgccttcc gccgccggtt tacccggacg ccagactacg
     6721 cggtcgccgg ttaccagcga acggatggta cggcgaatat tgcagcggtg aacgtcgcca
     6781 tcggcggatt ccacatcagc gtgcatacca aagcggctga tgacgatacc ttcatcaggc
     6841 tcgccaaaca gattgtcgtc gtagtcgggc ttctccttag acgttttaag acgacgctgg
     6901 tgattggcgt tcacgcggcg ctgctggcct ttggagagtt tatttttact caatcgtact
     6961 gactcctggt cgcccctgat gggcaaaaca tctatgatac acgcaattgt ggatcaatat
     7021 agtcactgtg aatgggtgga aaatagcatg agtgccaatg aaaacaacct gatttggatc
     7081 gatcttgaga tgaccggtct ggatcccgag cgcgatcgca ttattgagat tgccacgctg
     7141 gtgaccgatg ccaatctgaa tattctggca gaagggccga ccattgcagt acatcagtcc
     7201 gatgaacagc tggcgctgat ggatgactgg aacgtgcgca cccataccgc cagcgggctg
     7261 gtagagcgcg tgaaagcgag cacgatgggc gatcgggaag ctgaactggc aacgctcgaa
     7321 ttcttaaaac agtgggtgcc tgcgggaaaa tcgccgattt gcggtaacag catcggacag
     7381 gaccgtcgtt tcctgtttaa atacatgccg gagctggaag cctacttcca ctaccgttat
     7441 ctcgatgtca gcaccctgaa agagctggcg cgccgctgga agccggaaat tctggatggt
     7501 tttaccaagc aggggacgca tcaggcaatg gatgatatcc gtgaatcggt ggcggagctg
     7561 gcttactacc gcgagcattt tatcaagctg taaaattttt cctggtcacg taagcgccgg
     7621 aatccacttg cggcggcgtg attttgacgc taaattgaac gtcttgctga taatttcagc
     7681 acttgagata aaaacgcaaa aaaaactttt ttggggggtt gcagagggaa agatttctcg
     7741 tataatgcgc ctcccgtaac gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag
     7801 tacgcgggaa tagctcagtt ggtagagcac gaccttgcca aggtcggggt cgcgagttcg
     7861 agtctcgttt cccgctccaa aatttgaaaa gtgctgcaaa gcacagacca cccaatcgca
     7921 gtatttcgaa ttgcgacaag gcggcaactg aacgaatcct tgggaactta caaaagtatg
     7981 tgactaaggt gagtgaaggc agccaaggca gtagcggttc gaaagacgaa gatta
//
LOCUS       DAZPAC010000187         7968 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZPAC010000187 DAZPAC010000000
VERSION     DAZPAC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.187"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..239
                     /locus_tag="ACHUPM_003426"
     CDS             <1..239
                     /locus_tag="ACHUPM_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFR0871113.1"
                     /translation="LLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIVQLLLEHG
                     ASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            279..1142
                     /locus_tag="ACHUPM_003427"
     CDS             279..1142
                     /locus_tag="ACHUPM_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR0871114.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            1132..2679
                     /gene="fdrA"
                     /locus_tag="ACHUPM_003428"
     CDS             1132..2679
                     /gene="fdrA"
                     /locus_tag="ACHUPM_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFR0871115.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            2679..4037
                     /locus_tag="ACHUPM_003429"
     CDS             2679..4037
                     /locus_tag="ACHUPM_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR0871116.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAGPPITWEKMCGAMKGAVTGAL
                     VFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGVTSASMFMHIVKNKTYGNIAY
                     TNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDAMKIIGEIDLRLMLAQALHMG
                     DECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEFVASSDYFSGPTWMAMCKAAM
                     DAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPAQQVIGPMFAGYKPEDSGLDI
                     GDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSRQMREITLGENPNVTIPLLGF
                     MGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAGIVHPPFACFEKAILGWCERY
                     GV"
     gene            4180..5130
                     /locus_tag="ACHUPM_003430"
     CDS             4180..5130
                     /locus_tag="ACHUPM_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFR0871117.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADMVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            5140..6522
                     /locus_tag="ACHUPM_003431"
     CDS             5140..6522
                     /locus_tag="ACHUPM_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR0871118.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            6738..6812
                     /locus_tag="ACHUPM_003432"
                     /pseudo
     CDS             6738..6812
                     /locus_tag="ACHUPM_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6899..7948
                     /gene="yahK"
                     /locus_tag="ACHUPM_003433"
     CDS             6899..7948
                     /gene="yahK"
                     /locus_tag="ACHUPM_003433"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFR0871119.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1919 a         2019 c         2308 g         1722 t
ORIGIN      
        1 agcttttggc gcatacggag attaacgtta accagaccaa ccatgtaggc tggacgccgc
       61 tactggaagc gattgtgctt aatgatggtg gtattaaaca gcaggcgatt gtgcagttat
      121 tgctggaaca cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccactggaac
      181 tggcgcggga acggggcttt gaagagattg cgcagttact gattgccgca ggtgcataaa
      241 ccgggaggct ggctatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg
      301 cggattttct ggcgcagcgg gggcaaggac aggttgaaca ggtctttgcc agagcggtaa
      361 atatcgcact cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg
      421 cgccaaacag ttgtcggttg gcactcactc actttgatga tctgttccgg catggtgata
      481 aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca tcttcatata gagatgagtc
      541 gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg
      601 cctggctaca gtggcacgac attattcatc agcacctggg ggaaaatgaa accctgttta
      661 attatcgcgg cgataatccg ttttatcagg cgttaaataa agaattacat attaaacgac
      721 gggcagttat tcagacagta aacgataagc aaaatatcgc cgcagcggtc gccagtatga
      781 ttgggttagg gattggcctt acgccatcag ccgatgatta tttaacaggt ctggcgctta
      841 ttttatttat tcccgggcat ccggcagaaa aatacaaaga ggaattttat cgcggtctgc
      901 tacgcggcag aaataacacc acgttattaa gtgccataac gctggaagcc gcattacaac
      961 aacgctgccg ggaaaatatt catcgcttta ttcacaacat tatttatggc gtccctggga
     1021 attcaactca ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc
     1081 tgtatggcat ggccgatggt tgtgcgctga gccaaaccta cggagggaat tatgtcagtt
     1141 aaaatagtca ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg
     1201 cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac
     1261 aaaggtgtgc tgaaaaattt aggactgctg acgccgacgc tggaacaggc gaaaaacggc
     1321 gacctgatga ttgtcatcaa tggtaaatcg ggtgcggaca acgagcagtt gctggtggag
     1381 attgaagaac tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacgccact
     1441 attgccagcg ccaaaaagca tatcccggaa agtaacctgg cggtgatttc ggtcaacggt
     1501 ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt
     1561 tccgataacg tctcagttga agatgaactg gcgctcaagc agttggccca cgaaaaaggg
     1621 ctgctgatga tggggccgga ctgcggcacg gcgattatta acggcgcggc gctctgtttt
     1681 ggtaacgccg tgcgtcgcgg caatatcggt attgttggcg catccggcac cggcagccag
     1741 gagctgagcg tccgcattca tgaatttggt ggcggtgttt cacaactgat tggcaccggc
     1801 gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc tcgacgccat cgggatgctg
     1861 gaaaacgatc cgcaaactga aattatcgca ctgatttcaa aacctcccgc gcctgcggtg
     1921 gcccgcaaag tgctggaacg cgcacgtgcc tgccgcaagc cggtggtggt ctgcttcctc
     1981 ggtcgcgttg aaacgccggt ggatgagcag gggctacagt ttgcccgcgg cagcaaagag
     2041 gcggcgctaa aagcggtgat gctctccggc gtgaaacagg aaaatctcga cctgcatacg
     2101 cttaaccagc cgttgattgc tgatgtgcgt gcgcgtctgc aaccgcagca gaaatacatt
     2161 cgtggcctgt tctgcggcgg cacgctgtgc gacgaaacca tgttcgcggt gatggaaaaa
     2221 catggcgatg tctacagcaa cattcagccc gatccggaat tccgcctgca agatatcaac
     2281 cgcagcatca aacacacctt cctcgacttt ggcgatgacg acttcacgaa cggcaagccg
     2341 catccaatga ttgaccccac caaccgcatc agtcgcttga tcgaagaggc gcgcgatcca
     2401 gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc
     2461 ggctccacca tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag
     2521 ttgatcattc tcgcctatgt gctgggtacc gatcttgata cgccatcgtt agaacaacaa
     2581 agccagatgc tgcttgatgc cggagtgatt ctggcgagca gcagcaccaa taccggattg
     2641 ctggcgcgtg aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc
     2701 aaccattgaa cgttattaac gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc
     2761 atgtagaagt gactcaactc gactggacgc cgccggggca gggcaatatg caggtggtgc
     2821 aggcgctgga gcgcattatc cagtcgcatc cggtgctgat tggttttgat caggcgatta
     2881 acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc cgggccgccg atcacctggg
     2941 aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg
     3001 cgaaagatct cgacgaggcg gctgaactgg cggcctccgg ggagatcacc ttctcgccgt
     3061 gccacgaaca cgactgtgtg ggatcgatgg cgggcgttac ctcggcctcg atgtttatgc
     3121 acatcgtgaa aaacaaaacc tacggcaaca tcgcttacac caatatgagc gagcagatgg
     3181 cgaagatttt gcgtatgggt gccaacgatc agagcgtgat cgaccgcctc aactggatgc
     3241 gcgatgtgca ggggccaatg ctgcgcgacg cgatgaaaat tatcggtgag atcgatctgc
     3301 gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg ccataaccgc aataacgccg
     3361 ggacgacgct gctgattcag gcactgacgc cggggatcat tcaggcaggc tattccgtcg
     3421 agcaacagcg cgaagtgttc gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt
     3481 ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg catcgaatac agcaccgtgg
     3541 tcaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc
     3601 aatggtttac cggcccggcg cagcaggtga tcggcccgat gtttgccggt tataagccgg
     3661 aagattcggg gctggatatc ggcgacagcg ccatcaccga aacctacggt attggtggat
     3721 ttgctatggc gaccgcgcct gcaatcgtcg cgctggtggg cggcacggtg gaagaggcta
     3781 tcgatttctc ccgtcagatg cgtgaaatca ctctcggtga aaaccccaac gtcaccattc
     3841 cgctgctcgg ttttatgggc gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg
     3901 gcattctgcc ggtgatcaac accgccatcg cccataaaga tgcgggcgtc ggcatgattg
     3961 gcgcgggcat tgtgcatcca ccattcgctt gcttcgagaa agccattctc ggctggtgcg
     4021 aacgttacgg cgtctgactt acatgtttat gccggatgcg gcgtgaacgc cttatccggc
     4081 ctacggatgg cccgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
     4141 ggtcataacc tcatcaccct ctgtagcgga cataacaaca tgaaagagct tgtggtcgtt
     4201 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
     4261 gaggcggtga aagccgtggc cgatatggtg ctggaaatgc tggcttccga ttacgacatt
     4321 gtgctgaccc acggtaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
     4381 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcaga tacgcagggc
     4441 ggcatcggct atctgatcca acaggcgctg aataaccgac tggcgcgtca cggcgagaaa
     4501 aaagccgtca ccgtggtaac tcaggtggaa gtggataaaa acgatccagg ttttgcccat
     4561 cccaccaagc ccattggtgc attcttcagt gaaagccagc gtgacaaact acaaaaggca
     4621 aaccctgact ggtgttttgt tgaagatgcc ggacggggct atcgccgcgt ggtcgcctcg
     4681 ccggaaccga aacgtattgt cgaagcaccc gccattaagg cgctgatcca acaaggtttt
     4741 gtcgtaattg gcgcgggcgg cggtggaatt ccggtagtgc gtactgaagc gggggattac
     4801 caaagcgtgg acgcggttat tgacaaagat ctctctaccg cgctgctggc ccgtgaaatt
     4861 cacgccgaca ttctggtgat caccaccgac gtcgaaaaag tctgtatcca ctttggtaaa
     4921 ccgcagcagc aggcgctgga tcgggtggat attgccacca tgactcgcta tatgcaggaa
     4981 gggcattttt ctcccggcag catgttgcca aaaatcatcg ccagcctgac gttcctggag
     5041 cagggcggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctgcgcggt
     5101 gaaacgggca cccatattat taaaacgtaa ggacgtagga tgaaagaaag caatagccgc
     5161 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctat
     5221 gtgccgctcg cccatgcggc ggtagctggc accctaaact gcgaagcgaa caacaccatg
     5281 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     5341 gaaaacggtg tggcggtgca aacccgcacg gcgcgccaga ccgtggagat tcaggacggc
     5401 aaaattgtcg ccctgcgcga gaacaagcag catccggacg ccacgctgcc gcactatgac
     5461 gctggcggta agttgatgct acccaccact cgcgacatgc atattcatct cgacaaaacc
     5521 ttctacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccactat ccaggacatg
     5581 atcaaactca agcagaaaat gctgccggag ttacaaccgt atacgcagga gcgggcggaa
     5641 aaactgatcg atctattgca gtcgaaaggc accaccattg cccgcagcca ctgcaatatc
     5701 gaaccggttt ccggcctaaa aaatctgcaa aatttgcagg cggtgctggc gcggcgtcag
     5761 gcgggcttcg agtgtgaaat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg
     5821 gaacccttaa tgcgcgaagc gatgcaggcg ggtgcgcatt acgtcggcgg gctggacccg
     5881 accagtgtcg atggcgcgat ggaaaaatcc ctcgacacca tgtttcagat tgcgctggac
     5941 tacgacaaag gtgtcgatat tcacctgcac gaaaccactc cgtcgggcgt ggcagccatc
     6001 aattatatgg ttgaaacggt agagaaaacg ccgcaactga aaggtaagct gaccatcagc
     6061 cacgcctttg cgctggctac gctcaacgaa caacaggtcg atgcgctggc gaatcggatg
     6121 gcggcgcagc aaatttctat cgcctcgacg gtgccgattg gcacgctgca tatgccgctc
     6181 aaacagttgc acgacaaagg cgtaaaagtg atgactggca ctgacagcgt tatcgaccac
     6241 tggtcgcctt atggtctggg cgacatgctg gaaaaagcca atctctacgc gcagctctat
     6301 attcgtccta acgaacagaa cctctcccgt tcgctgtttc tagccactgg cgatgtattg
     6361 ccgctgaatg aaaaaggcga gcgtgtatgg ccaaaagcgc aggatgatgc cagctttgtg
     6421 ctggtggacg cctcctgttc cgccgaggcg gtggcgcgta tctcgccgag aaccgcaacg
     6481 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct
     6541 gcaacggtga attgtaagag atttaagccc cagggagtgg ggcgacctca atatgatagt
     6601 gtcggcagaa atggacatta tctttgagga atatgccctt attgacgttg tgtagataac
     6661 tatttgtcta cgtgaaaaga tcatcagttt tgccgatttt agtcttttac ggatagcaaa
     6721 tatcacactt ataggcgctc gccttatggg gaacgttgct gctctgtttc attcaggttc
     6781 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
     6841 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
     6901 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
     6961 acggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
     7021 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtaccggg
     7081 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
     7141 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgagga
     7201 cgggctggaa aactactgtg atcacatgat cggcacctat aactcgccga cgccggacga
     7261 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
     7321 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
     7381 caccacgtac tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgggcgtggt
     7441 cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg gggcacatgt
     7501 ggtggcattt accacttctg aggcaaaacg cgaagcggaa aaagccctgg gggccgatga
     7561 agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt tcgatttcat
     7621 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgtga
     7681 tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
     7741 cctgatcatg aaacgccgtg cgatagccga ttctatgatt ggcggcattc cagaaactca
     7801 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
     7861 cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
     7921 tatcgataat cgcacactaa cagattgaaa aaattaataa atacgctg
//
LOCUS       DAZPAC010000188         7958 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZPAC010000188 DAZPAC010000000
VERSION     DAZPAC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.188"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            163..317
                     /locus_tag="ACHUPM_003434"
                     /pseudo
     CDS             163..317
                     /locus_tag="ACHUPM_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            363..779
                     /gene="hicB"
                     /locus_tag="ACHUPM_003435"
     CDS             363..779
                     /gene="hicB"
                     /locus_tag="ACHUPM_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFR0871120.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            858..2264
                     /locus_tag="ACHUPM_003436"
     CDS             858..2264
                     /locus_tag="ACHUPM_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFR0871121.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2509..3654
                     /gene="ydcS"
                     /locus_tag="ACHUPM_003437"
     CDS             2509..3654
                     /gene="ydcS"
                     /locus_tag="ACHUPM_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFR0871122.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3672..4685
                     /locus_tag="ACHUPM_003438"
     CDS             3672..4685
                     /locus_tag="ACHUPM_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0871123.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4686..5586
                     /locus_tag="ACHUPM_003439"
                     /pseudo
     CDS             4686..5586
                     /locus_tag="ACHUPM_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5576..6370
                     /locus_tag="ACHUPM_003440"
     CDS             5576..6370
                     /locus_tag="ACHUPM_003440"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0871124.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            6392..7816
                     /gene="patD"
                     /locus_tag="ACHUPM_003441"
     CDS             6392..7816
                     /gene="patD"
                     /locus_tag="ACHUPM_003441"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR0871125.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         1849 a         1890 c         2196 g         2023 t
ORIGIN      
        1 ttcagttttg caaattagcg caaagaaatt ctggaatctt ccttcctgat tttgcattgc
       61 attctgccgt tgcggcgatt tagtgctatt ttcgagcaaa ttacacatag gggtaaacac
      121 taatgtttac tttattgata tactcagcgg cagggaggcg atgtgaaaca aagcgagttc
      181 agacgttggc tcgaatctca gggcgtcgat gtagcgaatg gccgcaacca tttgaaactc
      241 aggtttcatg ggaggcgcag tgtcatgccg cgtcactcct gcgtaaagca atcctgaaac
      301 aactcggttt gagttaatcg ccaattaaaa aggttaatga catgcgagag acagtcgaaa
      361 ttatgcgtta tcccgtcact cttacacccg cgccggaagg cggttatatg gtttcttttg
      421 tggatatccc tgaagcgttg acgcagggcg aaactgtcgc tgaagcgatg gaagcggcaa
      481 aagatgcttt actgaccgca tttgattttt attttgaaga taacgagctt atcccgttac
      541 cttcgccatt aaatagtcat gatcacttta ttgaagtacc tttgagcgtc gcctctaaag
      601 tattgctgtt aaatgctttt ttacagtcag aaatcactca gcaagagtta gccaggcgaa
      661 ttggcaaacc taaacaggag attactcgcc tatttaactt gcaccatgcg acaaaaatcg
      721 acgccgtcca gcttgcggca aaggcgcttg gcaaagagtt atcgctggtg atggtttaat
      781 tacagttaac gaaaagttgt catttttaac aactgatata gactgccgaa tcatctgcac
      841 ataattacga ttcgataatg aaaaaatacc agcagcttgc agaacaatta cgcgagcaga
      901 ttgcgtcggg tatctggcaa cccggcgatc gtttgccttc gttgcgtgac caggtggcgc
      961 tttcaggcat gagctttatg actgtcagcc atgcctatca gttgctcgaa agtcagggat
     1021 atattatcgc acgaccgcag tcgggttatt acgttgcgcc acaggcaata aaaatgccga
     1081 aagcgccagt cattccagtc actcgagatg aagcagtcga tatcaacact tatatttttg
     1141 atatgttgca ggccagtcgc gaaccgtcgg tcgttccgtt tgcctcggcc tttcccgacc
     1201 cgcgactttt ccccctccaa caactaaacc gctcgctggc gcaggtaagc aaaaccgcca
     1261 cagcgatgag cgtgattgaa aacttaccgc caggaaacgc agaactgcgt caggctattg
     1321 ctcgtcgcta tgccttacag ggcatcacca tttctcctga tgaaattgtc attactgccg
     1381 gggcgttaga ggcattaaac ctcagtttgc aagcggtaac tgaaccgggc gattgggtga
     1441 tagtagagaa tccttgtttc tacggtgcgt tgcaggcgct ggagcggcta cggctgaagg
     1501 cgttatcggt ggcgacggat gttaaagaag ggatcgatct tcaggcgctg gaactggcgt
     1561 tgcaggatta tccggtgaaa gcgtgctggc tgatgactaa tagccagaac ccactcggat
     1621 ttaccttaac gccgcaaaaa aaagcacaac tggtggcgtt actcaatcag tacaacgtaa
     1681 cgctgattga agatgacgtt tacagcgaac tttattttgg acgggaaaaa ccgctgcctg
     1741 cgaaagcgtg ggatcgccac gatggcgttt tgcattgctc ttcgttttcg aaatgtctgg
     1801 tgcctggttt tcgtattggt tgggtcgccg ccggaaaaca tgcacgtaaa attcaacgct
     1861 tgcagttgat gagtacgctt tccaccagct caccgatgca acttgcgctg gtggattacc
     1921 tttccacgcg ccgatacgac gcccatcttc gtcgcctgcg tcgccagctt gcggaacgta
     1981 aacaacgtgc ctggcaggca ctgctgcgtt atctgcctgc ggaagttaaa attcatcata
     2041 gtgacagcgg ttattttctc tggctggagc tccccgagcc gttagatgcc ggcgaattaa
     2101 gcctggcggc actgacgcat catatcagta ttgcgccggg taaaatgttt tctaccggtg
     2161 aaaactggtc acgttttttt cgttttaata ccgcgtggca gtggggagaa cgtgaagaac
     2221 aggcggtaaa acaattaggc aaacttattc aagaacggct gtaatagcgt ttaatttaat
     2281 tcctcttaga ttgggtaata tgaatttcga atagcagtca tatttcctaa ccccttgtct
     2341 atactccaga agataacctt acagacggca taatgcgcgg tagctcacaa cctgattaaa
     2401 ttttctcagg gacgaaggtg tgcctgcaag ccgccgtcta tggttaaaca aggagatatt
     2461 tttacggcac ggcggctgaa caattaatta cgacaggagt aagaccttat gagcaagaca
     2521 tttgcccgca gcagcctgtg tgcgctcacc atgacaataa tgaccgctca cgccgccgaa
     2581 ccacctacca atttagataa accggaaggg cgactggata ttatcgcctg gccgggatac
     2641 atcgaacgcg gacaaactga taaacaatac gactgggtaa cgcaatttga aaaagagaca
     2701 ggctgcgcgg tgaatgtgaa aaccgccgcg acttccgatg aaatggtcag tctgatgacc
     2761 aaagggggtt acgatctggt tacggcatcc ggcgatgcct cgctgcgttt gattatgggc
     2821 aaacgcgtgc agccgattaa taccgccttg attcccaact ggaaaacgct cgatccgcgc
     2881 gtggttaaag gcgactggtt taatgttggc ggcaaagttt acggcacacc ttaccaatgg
     2941 gggccgaacc tgctgatgta caacactaaa accttcccga cgccgccgga tagctggcaa
     3001 gtggtttttg ttgagcaaaa tctgccggac ggcaagagca ataaaggccg cgttcaggct
     3061 tatgatggcc ctatctacat tgcggacgct gcgttgttcg ttaaagccac tcagccgcag
     3121 ttgggcatca gcgatccgta tcaactaacc gaagaacagt accaggcggt gctgaaagtg
     3181 ctgcgcgctc aacacagttt gatccatcgc tactggcatg acactaccgt gcaaatgagc
     3241 gatttcaaaa acgagggtgt ggttgcttcc agtgcctggc cctatcaggc caacgccctg
     3301 aaagccgaag gccagcctgt tgctaccgtt ttcccgaagg agggtgttac cggttgggct
     3361 gataccacca tgctgcatag cgaagcgaaa catccggttt gcgcctacaa atggatgaac
     3421 tggtcattaa cgccaaaagt gcagggcgat gtggcggcct ggtttggctc gttaccggta
     3481 gtgccggaag ggtgtaaagc cagtccgtta ttaggcgaaa aaggttgtga aaccaacggt
     3541 tttaactatt tcgacaaaat cgccttctgg aaaacgccta tagcagaagg gggcaagttt
     3601 gttccctaca gtcgctggac gcaggattac attgccatta tgggcggtcg ctaacttcgc
     3661 tggggtgctt tatgacgtac gcagtggagt ttgacaacgt ctcgcggttg tacggtgacg
     3721 tgcgcgcagt agatggcgtc agtattgcga taaaagatgg tgagttcttc tctatgctgg
     3781 ggccgtccgg ctccggcaaa accacctgcc tgcgcctgat tgctggcttc gaacagcttt
     3841 ccggcggggc tatctctatc tttggtaaac ccgccagcaa tctgccaccg tgggagcggg
     3901 acgtgaatac tgtctttcag gactacgcgc tatttccgca tatgtcgatt cttgacaatg
     3961 tcgcctatgg gctgatggtc aaaggcgtga ataaaaagca gcggcacgca atggcgcaag
     4021 aggcgctgga gaaagtggcg ttggggtttg tacagcaacg taaaccgtca caactttctg
     4081 gtggtcagcg ccagcgggtt gctatcgcca gagcattggt gaatgaaccg cgcgtattgc
     4141 tgttggatga accgctcggc gcactggatc tcaaattgcg tgagcagatg cagctggaac
     4201 tgaaaaaact gcaacagtct ctcggtatca cttttatctt cgttacccac gatcagggcg
     4261 aagcgttatc gatgtccgat cgtgtggcgg ttttcaataa tggacgcatt gagcaggtcg
     4321 attccccgcg cgatctctat atgcgcccgc gcacgccgtt tgttgccggg ttcgttggta
     4381 catcgaatgt ttttgatgga ctgatggcag agaaactttg tggcatgacg ggaagcttcg
     4441 ccctgcgacc ggaacatatc cgcctcaaca cccctggtga actgcaggcc aatggcacga
     4501 tccaggcggt gcaatatcag ggcgcggcaa ctcgttttga actgaaattg aacggcggtg
     4561 aaaaactgct tgtgagtcag gccaatatga caggcgaaga actgcctgcc acgctcacgc
     4621 ccggacaaca ggtgatggtt tcctggtcgc gtgatgtgat ggtgccgctg gttgaggaga
     4681 ggtgaatggc gatgaatgta ttgcaatcac cttcacgtcc aggtctgggg ctgtttttac
     4741 tgctgcttgg cccgctaatg tggtttggca ttgtctattt cggctcgctg ctgacactgt
     4801 tatggcaggg attttatact tttgacgatt tcaccatgtc ggtaacgccg gaactgacgc
     4861 tggcgaatat ccgtgcgctg tttaatccgg cgaattacga catcattctc cgcacgctga
     4921 ctatggctgt ggcagtcact atcgccagcg ccattctggc ttttccaatg gcgtggtata
     4981 tggcgcgcta taccagcggg aaaatgaaag cgtttttata ttgcggtaat gttgccgatg
     5041 tgggcgagct acattgttaa agcctatgcc tggacgttat tgctggcaaa agatggcgtg
     5101 gctcagtggt ttttacaaca tcttgggctg gaaccactgc tgactgcgtt ccttacatta
     5161 cctgcggtgg gcggaaatac gctgtcaact tccgggctgg ggcgctttct ggtgtttctc
     5221 tatatctggt tgccgttcat gatcctgccc gttcaggcgg cgcttgagcg tttgccgccg
     5281 tcattgttgc aggcgtcggc tgatctcggc gcacgtccac gacaaacctt tcgctatgtg
     5341 gtgctgccgc tggcaatccc gggtattgcc gctggctcta tctttacctt ctcactcact
     5401 ctgggcgatt ttatcgtccc gcagctggtt ggtcctccag gatattttat cggcaatatg
     5461 gtttattccc agcagggggc gattggcaat atgccgatgg cggcggcatt caccttggtg
     5521 ccgattattc tcatcgcact gtacctggcg ttcgtgaaac gtctgggagc gttcgatgca
     5581 ctctgaacgc gcaccgtttt tcctcaaact ggcggcctgg ggcggcgttg ttttcctaca
     5641 ttttcccatc ctgataatcg ccgcctatgc gtttaacact gaagatgcgg cgtttagttt
     5701 tccaccgcag ggcctgacgc tgcgctggtt tagcgtggca gcacagcgta gtgatattct
     5761 tgatgccgtg acactgtcac ttaaagtggc ggcgctggcg acattaattg cgctggtgtt
     5821 agggacgctg gcagctgccg cgctgtggcg acgagacttt ttcggcaaaa acgccatttc
     5881 gctgttactg ctgctgccca ttgcgctgcc gggcagcgtc actggtctgg cgttattaac
     5941 cgcctttaaa accatcaatc tggagccggg atttttcacc atcgtggtcg gtcatgcgac
     6001 tttttgtgta gttgtggtgt ttaacaatgt catcgcccgt tttcgccgca cctcctggag
     6061 tctggttgag gcgtcaatgg atcttggggc caatggctgg caaaccttcc gctacgtagt
     6121 gttgccgaat ctcagttcgg cgttactggc aggaggaatg ctggcgtttg ccttgtcgtt
     6181 cgatgaaatc atcgttacga cctttacggc aggtcatgaa cgaacgttac tgttgtggtt
     6241 gctcaatcag cttgggcgac cgcgtgatgt accggtaact aacgtggtgg cactgctggt
     6301 tatgttggta acaaccttgc cgatcctggg ggcctggtgg ctaacccgcg aaggcgacaa
     6361 tggtcaataa ccactgatac aggaatatgc tatgcaacat aagttactga ttaacggaga
     6421 actggttagc ggcgaagggg aaaaacagcc tgtctataat ccggcaacgg gggacgtttt
     6481 actggaaatt gccgaggcat ccgcagagca ggtcgatgct gctgtgcgcg cggcagatgc
     6541 agcatttgcc gaatgggggc aaaccacgcc gaaagtgcgt gcggaatgtc tgctgaaact
     6601 ggctgatgtt atcgaagaaa atggtcaggt ttttgccgaa ctggagtccc gtaattgtgg
     6661 caaaccgctg catagtgcgt tcaatgatga aatcccggcg attgtcgatg tttttcgctt
     6721 tttcgcgggt gcggcgcgct gtctgaatgg tctggcggca ggtgaatatc ttgaaggtca
     6781 tacttcgatg atccgtcgcg atccgttggg ggtcgtggct tctatcgcac cgtggaatta
     6841 tccgctgatg atggccgcgt ggaaacttgc tccggcgctg gcggcaggga actgcgtagt
     6901 gcttaaacca tcagaaatta ccccgctgac cgcgttgaag ttggcagagc tggcgaaaga
     6961 tatcttcccg gcaggcgtga ttaacatact gtttggcaga ggcaaaacgg tgggtgatcc
     7021 gctgaccggt catcccaaag tgcggatggt gtcgctgacg ggctctatcg ccaccggcga
     7081 gcacatcatc agccatgccg cgtcgtccat taagcgtact catatggaac ttggtggcaa
     7141 agcgccagtg attgtttttg atgatgcgga tattgaagca gtggtcgaag gtgtacgtac
     7201 atttggctat tacaatgctg gacaggattg tactgcggct tgtcggatct acgcgcaaaa
     7261 aggcatttac gatacgctgg tggaaaaact gggtgctgcg gtggcaacgt taaaatctgg
     7321 tgcgccggat gacgagtcta cggagcttgg acctttaagc tcgctggcgc atctcgaacg
     7381 cgtcagcaag gcagtagaag aggcgaaagc gacagggcac atcaaagtga tcactggcgg
     7441 tgaaaagcgc aagggtaatg gctattacta tgcgccgacg ctgctggctg gcgcattaca
     7501 ggacgatgcc atcgtgcaaa aagaggtatt tggtccagta gtgagtgtta cgcccttcga
     7561 caacgaagaa caggtggtga actgggcgaa tgacagccag tacggacttg catcttcggt
     7621 atggacgaaa gatgtgggca gggcgcatcg cgtcagcgca cggctgcaat atggttgtac
     7681 ctgggtcaat acccatttca tgctggtaag tgaaatgccg cacggtgggc agaaactttc
     7741 tggttacggc aaggatatgt cactttatgg gctggaggat tacaccgtcg tccgccacgt
     7801 catggttaaa cattaagtta gatgttagtg gctgttatag gacggccact atcgttttag
     7861 gttctgagtt taatggcggt taagtattag aatgattaca ttaataacta gcgtaaaaat
     7921 taggctaaat gtttgatggc gatttttcat cctgtcac
//
LOCUS       DAZPAC010000189         7899 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZPAC010000189 DAZPAC010000000
VERSION     DAZPAC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.189"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..359
                     /locus_tag="ACHUPM_003442"
     CDS             <1..359
                     /locus_tag="ACHUPM_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HFR0871126.1"
                     /translation="GRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGE
                     RSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLR
                     KDIMRRLTQDVCEEIE"
     gene            complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="ACHUPM_003443"
     CDS             complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="ACHUPM_003443"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR0871127.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3127..3960
                     /gene="ppsR"
                     /locus_tag="ACHUPM_003444"
     CDS             3127..3960
                     /gene="ppsR"
                     /locus_tag="ACHUPM_003444"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR0871128.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4117..5163
                     /gene="aroH"
                     /locus_tag="ACHUPM_003445"
     CDS             4117..5163
                     /gene="aroH"
                     /locus_tag="ACHUPM_003445"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR0871129.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5295..5486
                     /gene="hemP"
                     /locus_tag="ACHUPM_003446"
     CDS             5295..5486
                     /gene="hemP"
                     /locus_tag="ACHUPM_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR0871130.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5490..6926)
                     /gene="selO"
                     /locus_tag="ACHUPM_003447"
     CDS             complement(5490..6926)
                     /gene="selO"
                     /locus_tag="ACHUPM_003447"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR0871131.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="ACHUPM_003448"
     CDS             complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="ACHUPM_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR0871132.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2020 a         2075 c         1875 g         1929 t
ORIGIN      
        1 ccggacgcaa aaaagatatt attgtccgcg gcggcgaaaa tattagcagc cgtgaagtag
       61 aagatatttt attgcagcat cctaaaattc acgatgcctg tgtggttgca atgcccgatg
      121 aacgtttagg tgaacgatca tgcgcttatg tcgtgctgaa agcgccgcat cattcattat
      181 cgctggaaga ggtggtggct ttttttagcc gtaaacgggt cgcaaaatat aaatatcctg
      241 aacatatcgt ggtaatcgaa aaactaccgc gtactgcctc aggtaaaata caaaagtttt
      301 tgttaagaaa agatattatg cggcggttaa ctcaggatgt ctgtgaagag attgaataag
      361 tttcatcttc ggggatcaca taaccccggc gactgaacgc cgccggggat ttattttatt
      421 tcttcagttc agccaagctt aaccaggttt gcaccacggt gtccgggttc agagacaggc
      481 tatcgatccc ctcttccatc aaccatgcgg caaagtcttc gtggtcggac ggaccctgac
      541 cgcaaatccc gacatatttg ccctgtttct tcgcggcacg gatagccatc gacagcagtg
      601 ctttcaccgc atcgttgcgc tcatcgaaca attcagacac cacgccggag tcacggtcca
      661 gaccgagcgc cagctgcgtc atatcgtttg agccaattga gaagccgtcg aaatattcga
      721 ggaactgctc ggccagcaag gcgttggacg ggatttcaca catcatgatg attttcagcc
      781 cgttctcacc acgcttcagc ccctgacgcg ccagttcctc aaccaccgct ttcgcctgat
      841 ctacggtacg cacgaacggg atcatgatct caacgttggt cagccccatg tcgttgcgca
      901 cacgtttcac tgcttcgcac tccagcgcga agcagtcgcg gaagctgtcg gaaacatagc
      961 gtcccgcgcc acggaagccg agcatcgggt tctcttcatc tggctcgtaa cgctcaccac
     1021 cgaccaggtt ggcatattcg ttcgatttaa aatcagagag acggacaatg acgcgcttcg
     1081 gataaaacgc ggcacccagc gtcgcgatcc cttcagtcag acgaccaacg taaaattcac
     1141 gcggagaatc aaaacctttc atcatctcgc ggatttcatt ttgcaactgc ggttcctgat
     1201 cgtcaaactc aagcagtgcg cgtgggtgga cgccaatcat acggttgatg ataaattcca
     1261 gacgcgcaag gcccacgcct tcgttcggca ggcaggcgaa gtcgaaagca cggtccgggt
     1321 taccgacgtt catcatcact ttcaacggca gatccggcat catttctacg ctggagcttt
     1381 tcacgctaaa ttccagcaac tccgcataga cgtaaccggt atcaccttcg gcacaagaaa
     1441 cagtgacgtt ctcaccgtct ttcatccgtt ctgttgcatc accacagccc actaccgccg
     1501 gaatgcccag ttcacgagcg atgatcgccg cgtgacaggt acgaccgcca cggttggtga
     1561 cgatggcaga tgctttcttc atgatcggtt cccagtccgg gtcggtcatg tcagtgacca
     1621 gcacgtcacc aggttcgatg cggttcattt cgctgatctc atggatgact ttcaccggac
     1681 ccgcgccgat gcgatgaccg atagcacggc cttcggcgat aatcttaccc tgtgaatgca
     1741 gcgtataacg ctccatgacc tgaccgcgtg agcgcacggt ttccggacgc gcctgcacaa
     1801 tgaacagttt gccagtgtgg ccatctttcg cccactcaat atccatcggg cgaccgtagt
     1861 gtttctcaat ttgtacggcc tgttttgcca gttcctgcac ttcttcgttg gtcagcgaga
     1921 agatgtcacg ctgttcctgc ggtacgtctt cgattttaac ctgcttgccg tgctcctggg
     1981 tcggcgcgta aaccatgcgg atttttttcg accccatggt gcgacgcacg atagccgggc
     2041 gattcgccgc cagtgtcggt ttatggacgt aaaactcatc cgggttaacc gcaccctgca
     2101 cgaccatttc cccaaggccc catgcggaag tgataaacac cacctggtca aagccggatt
     2161 cggtatcaat ggagaacatc acgccagaag atgcgaggtc ggagcgcacc atccgctgaa
     2221 caccggcaga gagcgccacg ccacggtgat cgtaaccctg gtgcacacga taagagatgg
     2281 cgcgatcgtt aaacagagaa gcaaatacat gcttcactgc cacgagaacg gcgtcaaaac
     2341 cctgaacgtt gaggaaggtt tcctgctgac cggcaaaaga agcgtccggc atatcttctg
     2401 cggtggcgga ggagcgcacc gcaaaagagg cgttttcgtc atcggcggaa agctgtgcat
     2461 aggcttcgcg gatggcgttt tccagctcag gctggaaggg agtgtcgata atccactggc
     2521 ggatttgcgc gcccgctttc gcaagctgag taacatcgtc aatatccgtt ttatccagca
     2581 gttcataaat gcgctggttt acgccgcttt ggtccagaaa ctggttaaac gcgtcggcgg
     2641 ttgtggcgaa accattcgga acggaaacac ccattccgga aaggttagta atcatttcac
     2701 ccagggaggc atttttgccc ccaaccctgt ctacatcatt catgccgagt tggttatacc
     2761 aaagcaccag cggtgacgag ccattgttgg acatcgaaca atccttttgt gataaatgaa
     2821 cggtttgaga aacacatttc tgcgcattta tctttgcata tttaaccgga tgaaaaaaac
     2881 ggtgaatcgt tcaagcaaat atattttttt actttttaag actgatccca gcgttgcgca
     2941 aatctgctct cttcgacaat ttccacataa accatcggta taaacgaaac cataaaaatg
     3001 aaatgctgtt ttcataaaaa ataaaattga aggttcattt tataaaccag acataacgtc
     3061 ctacgcttct gtgcgttttt aatttatgct ttcatagaat tatgtctgca tcacgggaag
     3121 aacaaaatgg ataatgctgt tgatcgccac gttttttata tttctgatgg tacggcaata
     3181 actgcggagg tattaggaca cgcagtaatg tcacaatttc ccgtcactat cagcagcatt
     3241 acgctgccgt ttgtcgaaaa tgagagccgt gcacgggcag tgaaggatca gattgacgca
     3301 atttatcacc agacaggtgt gcgcccgctg gtcttctact ccatcgtgtt gccggagatt
     3361 cgcgccatca tcttgcaaag tgaaggcttt tgccaggata tcgttcaggc gctggttgcc
     3421 ccgctacaac aagagatgaa actggatcca acgccgattg ctcatcgtac ccatggcctt
     3481 aaccctaata atctcaataa atatgatgcg cgcattgcgg cgattgatta caccctcgcc
     3541 cacgatgacg gcatttcgtt gcgcaatctg gatcaggctc aggtgatcct gctcggtgtt
     3601 tctcgctgtg gtaaaacccc caccagtctt tatctggcaa tgcaatttgg tatccgcgcg
     3661 gcaaactacc cctttattgc cgacgatatg gataatctgg tgctacccgc gtcgctcaaa
     3721 ccgcttcagc ataaattgtt cggcctgact atcgacccgg aacgtctggc ggcgattcgc
     3781 gaggaacgtc gggagaacag tcgctatgcc tcgcttcgtc agtgcaggat ggaagtcgcg
     3841 gaagtggaag cgttgtaccg taaaaatcag atcccgtgga ttaacagtac caattattcg
     3901 gtagaagaga ttgccaccaa gatcctcgat atcatgggcc ttagtcgccg aatgtactag
     3961 agaactagtg cattagctta tttttttgtt atgatgctaa ccacccggcg aggtgtgaca
     4021 cacctcgcac ttgaaatcag cagcgattgg tttatcgtga tgcgcatcac ttcccggcag
     4081 tcctgccgta gaagcaacaa atttctgaga cttgtaatga acagaactga cgaactccgt
     4141 actgcgcgta ttgagagcct ggtaacgccc gccgaactcg cgctacggta tcccgtaacg
     4201 cctggcgccg ccacccatgt caccgactcc cgccgcagaa ttgaaaaaat actcaatggt
     4261 gaagataagc gactgttggt cattattggc ccctgctcga tccacgatct caccgctgca
     4321 atggagtacg ccccccgtct gcagtcgctg cgcaaccagt accagtcacg gctggaaatc
     4381 gtaatgcgca cctattttga aaaaccacga actgttgtcg gctggaaagg actaatctcc
     4441 gatccagatt taaacggcag ctatcgggta aatcacggtc tggagctggc gcgcaaatta
     4501 cttttacagg taaatgagct gggcgtccca accgcgactg agttcctcga tatggtgacc
     4561 ggtcagttta ttgctgattt aatcagttgg ggcgcgattg gcgcacgtac taccgaaagt
     4621 cagatccacc gtgaaatggc ttcggcactc tcctgtccgg taggttttaa aaatggtacc
     4681 gatggcaata cgcggattgc tgtggatgct atccgcgcag cccgcgccag ccatatgttc
     4741 ctctcgccag acaaaaatgg tcagatgacc atctatcaga ccagcggcaa cccgtatggc
     4801 cacattatta tgcgtggcgg caaaaaaccg aattatcatg ccgatgatat cgccgcagcc
     4861 tgcgatacgc tgcacgagtt tgatttacct gaacatctgg tggtggattt cagccacggt
     4921 aactgccaga agcagcaccg tcgccagtta gaagtttgtg aggatatttg tcagcaaatc
     4981 cgcaatggct ctacggcgat tgctggaatt atggcggaaa gtttcctgct cgaaggaacg
     5041 caaaaaatcg tcggcagtca gccgctcact tacggtcaat ccattaccga cccgtgtctg
     5101 ggctgggaag ataccgaacg cctggtcgaa aaactcgcct ctgcggtaga tacccgcttc
     5161 tgaatgcgtg cccattcctg acggaatgag catttctgcg caacttgttg tcttctcaac
     5221 aaattactgc ttgctctggc ctgccataat attgataata agaatcattg ttatatcaat
     5281 tattattaat ctttatgcgt tatacggata gcagaaaact cacgcctgaa acggacgcca
     5341 atcacaaggc cgcttccccg cagcccatcc ggcgaattac cagccagaca ctgttaggtc
     5401 cggatggcaa actgattatc gatcatgacg ggcaagaata tctgctccgt aaaactcagg
     5461 ctggcaagct gctgttaact aagtagcctt taactcgagc agctgacttc cagccgttta
     5521 ccccagtcag gtggacgact gacatagtca tcatctctat cgctgaaagg atttcgcaac
     5581 gccccatgca ggcggtgcaa ttccgtcata tcaccctttt ctgccgcttc aatcgcccgt
     5641 tgcgccaacc agttgcgcaa taccagagcg gggttaacgc tttgcatcaa ctgctgacgc
     5701 tcactatcgc taacctcgtc ttgttgcaaa cgcccccgat aacgggcaaa ccagccatca
     5761 aatgccgcac gatcaataaa ctcatcacgt agcggtgacg ccgtgctgtg ctgctcggtc
     5821 agactcagca tgcggaatgt gcgggtataa tcgctgcgct ctcgcgccat cagactgaat
     5881 aattcattca gtagcgcgtt atcctctttt tgctccgtga tgaagcccag tttctgccgc
     5941 atccgttgtc cataatgcgt caacaaaacc tgctgatagc tgtccagggc ctcattcagg
     6001 gcatctacgg caacaaatgg tgacaatgtc tgcgccagac gctgtaaatt ccacaacgcg
     6061 acagcaggtt gattatcaaa actataacgc ccttgatgat ccgagtgatt acaaataaaa
     6121 ccgggttcgt aatcatcaag aaaaccaaac ggcccgtaat caagcgtcag tcccagcagc
     6181 gacatgttgt cggtattcat caccccatgt gcaaagccga ccgtctgcca ttgggcaatt
     6241 aacgacgcgg tacgtgcgac aacatcgcta aaccagagac ggtatttgtc ctcatcatct
     6301 tcaagatgtg accagtaatg acgaatagca aagtcagcta actgacgaac cttttccgac
     6361 tcgcggcggt agtaaaaatg ttcgaaatga ccaaagcgca gatgacttgg tgccacacgc
     6421 atcagcatcg cgcctggctc cgccgtttcc cgatacactg gcgaatcgct ggtgacgata
     6481 cttaacgcgc gggtcgtcgg aatgcccaga taatgcatcg cctcactggc gagactttct
     6541 cgtatcgtcg aacgtaaaac cgcccgtcca tcacccattc gcgaataagg cgtcaggcca
     6601 gcacctttca gatgccagtc cattgtagtg ccatcagcaa gcagttgttc gccgagtaaa
     6661 atgccgcgcc catcacccag ttggcccgcc cagacgccga actgatgacc actgtaaacc
     6721 tgcgccagtg gtgacatacc aggcagtagg gcttcgccgc cccagacacc tgcgccattt
     6781 ttaaacagcg acgatggaat actcagcgtg ttagccagtt cggtattatg ccaaatcagc
     6841 cgggcattat ttaaaggcgt aggggaaagt gctgtatagg tttctggcaa ttcatcgcgc
     6901 cagcgggtaa caaaagacag ggtcatagat cctcctgttt gatagtgtag acggttactc
     6961 tcgtcttaaa caccagcaaa cagaagggtt atcgctgaac caacgtcgtt atctgattta
     7021 tgggaacggc aggccacagg catccctgga gagcatggaa atcgaagggg gtaatctgtg
     7081 ccaatatttc tgccgtatca atgcctcccg caatgatgca attgcaacaa ggggaaattt
     7141 gcgcctggat cgcacgaata aacggttcga aggagcggtg cgtgatttgt tgctgaataa
     7201 aacttttatc cagcatgacg cgggtgaaaa gaccatcaaa aaccgctttc attgtgctat
     7261 tgcctgctcc caaattacct aacacaaggg gatacacttg cgataaggat aaaagaccgc
     7321 gattatcttt tccttcattg agatgcgggt aattctcatt aatcaacagt tcaataaacg
     7381 gatattttaa tagttcgcct gcgtaattat ccctctctaa taataatgta gccacttgtg
     7441 gcgttaaatt aagccaggca aagagtttat gttgaataaa aaaatgttgg catgatttta
     7501 gtaattccaa ctgctctgaa aataattgcc aatgttgctc ctcggtaagt tgcgctataa
     7561 cccgactcgt cgggatacgc accgtgccat cttcactgga gaaatgggta attaactcaa
     7621 cgccgacaag gacttgctgg ttatctctta tcggcaggaa atagcaatca gaatgataaa
     7681 gattctccaa aaaaatcttc atggtaaccg tccctctcga aggatgtttt tcagtatccg
     7741 gctaaaaggg atgaggccat aagacataac aattacagaa ggagtaactt tcatttgttc
     7801 catgttaacc actttttcag gggtcctttt tttagattat cctgattata aacgaataat
     7861 ccatttacgg aatttttgtc tgcaaaatac tactgtatt
//
LOCUS       DAZPAC010000190         7826 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZPAC010000190 DAZPAC010000000
VERSION     DAZPAC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.190"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..791
                     /locus_tag="ACHUPM_003449"
                     /pseudo
     CDS             1..791
                     /locus_tag="ACHUPM_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            1154..2191
                     /gene="dusA"
                     /locus_tag="ACHUPM_003450"
     CDS             1154..2191
                     /gene="dusA"
                     /locus_tag="ACHUPM_003450"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR0871133.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2325..2567
                     /gene="pspG"
                     /locus_tag="ACHUPM_003451"
     CDS             2325..2567
                     /gene="pspG"
                     /locus_tag="ACHUPM_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR0871134.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2733..3716)
                     /locus_tag="ACHUPM_003452"
     CDS             complement(2733..3716)
                     /locus_tag="ACHUPM_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR0871135.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3799..5214
                     /gene="dnaB"
                     /locus_tag="ACHUPM_003453"
     CDS             3799..5214
                     /gene="dnaB"
                     /locus_tag="ACHUPM_003453"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0871136.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5267..6346
                     /gene="alr"
                     /locus_tag="ACHUPM_003454"
     CDS             5267..6346
                     /gene="alr"
                     /locus_tag="ACHUPM_003454"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR0871137.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6599..7792
                     /gene="tyrB"
                     /locus_tag="ACHUPM_003455"
     CDS             6599..7792
                     /gene="tyrB"
                     /locus_tag="ACHUPM_003455"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR0871138.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1915 a         1865 c         2038 g         2008 t
ORIGIN      
        1 ttgtttattc aacgaatcac gcataattta tggcatgcac gtaaaaaaat gtgtttttaa
       61 ttgagcaaat aaatgatctc aaaatgtggg tcaataagta tattgacgat tgcactgatg
      121 aggatttaaa cgatcgtgac tttattgcat cagttgttga ccgggctatt tttcatttcg
      181 cgattaatag tatatgtaat cctggggata ataaagatgc gactcccatt gaacaatgta
      241 cttttgatgt agaaactaag aatggccttc cctccacggt tcagctattt tatgaggaat
      301 ctaaggataa tgaaccttta gcgaatatac attttcaagc aataggttct ggttttttaa
      361 cgtttgttaa tgcctgccag gaacatgatg acaacagctt aaaattattt gcttcactgt
      421 taatttcact ttcatattct agtgcctacg cagatttatc agaaacagtg tatattaatg
      481 aaaataatga gagctacctg aaagctcagt ttgaaaaatt atctcaacgt gatatgaaga
      541 agtacctggg agagatgaag cgtctggctg atgggggaga aatgaatttt gatggctatc
      601 tggataagat gtcacatctg gtgaatgaag gaacgctcga tcctgatatt ttaagcaaaa
      661 tgcgagatgc tgcaccacaa ttaattagct tcgcgaagtc gtttgaccca acctcaaagg
      721 aagagattaa aatacttaca gacacttcta aattaattta tgatttgttc ggggttaaat
      781 cggagaaata atatgtgaag ttcttcgata gtatggaagg cattatataa aaggacccaa
      841 tatttattgg gttctttttt ctctatcaat gctattagca gggagatata tcaccagagt
      901 ttaatgtgtg attttttatt tatcgtcgaa cctggattgt ttatcattgg ccttaacaaa
      961 gttaacggct aataagatta tttccatcac ttcgtgagag cttcatgcct tgagaggatc
     1021 tcaattttct tttgcaatga gacaggcgct tcctgttgtt atggtatagt accccgctat
     1081 tgagcctcct gaatagtgat gctgaataac ataaccccat gatatatcga taaaataatc
     1141 tctacatttg aaaatgcacg gtaattctga aatgcaaaaa atcaaccaaa ccagcgcaat
     1201 gcctgaaaaa actgacgttc actggagtgg tcggtttagc gttgcaccaa tgctcgactg
     1261 gacggacaga cattgccgct atttcttgcg tctgctttcc cgcaatacgt tgctgtatac
     1321 cgaaatggtg accacagggg cgattattca cggtaaaggt gattacctgg cgtacagtga
     1381 agaagaacat ccggtagcgt tgcaactcgg cggtagcgat ccggcggcgc tggcacagtg
     1441 tgcgaagctg gcagaagcgc gtggatatga tgagatcaac ctgaatgtcg gctgcccgtc
     1501 tgaccgggtg cagaacggca tgtttggtgc gtgtctgatg ggtaatgcgc agctggttgc
     1561 cgactgcgtg aaagcgatgc gcgatgtggt gtcgattcca gtgacggtga aaacgcgtat
     1621 tggcatcgat gaccaggaca gctatgaatt tctctgcgat ttcatcaaca ccgtttccgg
     1681 caaaggcgag tgtgagatgt ttatcatcca cgcacgtaaa gcctggcttt cggggttaag
     1741 cccgaaagaa aaccgtgaaa tcccgccgct cgattatccg cgtgtgtatc aactgaagcg
     1801 tgactttccg catctgacaa tgtcgattaa cggtggtatc aagtcgctgg aagaggccaa
     1861 agcacacctg caacatatgg atggcgtgat ggtcgggcgc gaggcgtatc agaatccggg
     1921 tattctggcg gcggtagacc gggagatctt tggttcctcg gataccgatg ccgatccggt
     1981 ggcggtagtg cgcgccatgt atccgtacat tgagcgtgaa ctcagccagg ggacgtatct
     2041 cggccatatt acccggcata tgttgggctt gttccagggt attcctggcg cgcggcagtg
     2101 gcggcgttat ttaagtgaaa atgcccataa agcgggtgca gacattaatg tgctggaaca
     2161 cgcgctcaaa ctggtggcgg ataagcgtta acttttcacc aaaaagtagt caaattcacc
     2221 acgccctgcg caccgtcgcg gggcgttttg ctgttaaatc aatagattat ttttggcatg
     2281 attcttgtaa tgccagcaag agatttcata tttgggagag catcatgctg gaactacttt
     2341 ttgtgattgg cttttttgtc atgctgatgg tcaccggcgt ttcgttgctg ggcattatcg
     2401 ccgcgctggt tgtggcgacg gccattatgt tcctcggcgg tatgctggca ttgatgatta
     2461 agttgctgcc gtggttacta ctggcgattg cggtggtgtg ggttattaag gcgattaaag
     2521 caccaaaagt gccgaaatat cagcgttatg accgctggcg ttactaaggg attgtgcgga
     2581 tgatcacaac ctaaggtttt atccttagaa caaaatagga attgataatc aaatctgtca
     2641 ctattgcgcc tctaacagat tcatcgtgct gtaccctaca tacagccgaa ctataaaaag
     2701 aaagggcttc ccaggtggaa gccctatttc ttttatggaa tcaacaggct ggaaccttgc
     2761 gtcgcccggc tttccagaat ctcatgcgca cgctgcgcat ccttcagcgg atatttctgc
     2821 tgctcggcga catcgacctt aatcacaccg ctggcaatca aagagaacag ttcattactg
     2881 gcctcggtta attcctcccg cgtggtgata tagccttgca gggaagggcg tgtcacatac
     2941 aacgagcctt tttgattgag aatgcctaag ttcacaccgg taaccgcacc tgatgagttg
     3001 ccaaaactga ccattaagcc gcggcgttgc aggcaatcca gcgaccgttc ccaggtgtct
     3061 ctgcccacgg aatcgtacac cacgcgcact ttcttaccgc cggtgatctc ttttaaccgc
     3121 tcgaccagat cctcttcacg atagttaata acctgccacg cgcccgcttt tagcgcgctc
     3181 tgcgcttttt gcgcggttcc tacggtgccg ataagtttcg cgcccagggc ttttgcccac
     3241 tggcaggcaa ttaagccaac gccgccagcc gctgcgtgga acaggaactg ttcatcgggt
     3301 ttaatttcat aggttttgcg cagcagataa taaaccgtta agcctttcag gaaggatgcc
     3361 gcagcttgct caaaagaaat tgccgcaggc agaatcgccg ctttatccgc attaatgtta
     3421 tgcacagagc tgtaagcgcc taacgccgac tgcgcataga ctacacgatc gcctgcctta
     3481 atatgcttta caccactgcc gactttactc acgatgcctg ccgcttcggt acctaatccg
     3541 ctgggtagtg atggcggcgg gtaaaggccg ctgcggatat acgtgtcgat aaaattgatg
     3601 ccgatggctt tattttcgac ctggatttca ttctctgccg gatcggcagg agtgaactct
     3661 acggcttgaa gtacttccgg gccaccgtgc ttgtgaaatt caattcgtgt tgccatgtgt
     3721 cctccagaac gtatcgtcag ggtctgcttc atatggtaaa gtttcgaccc attctttatc
     3781 tcggtaactc cattcactat ggcaggaaat aaacccttca acaaacagca ggctgaaccc
     3841 cgcgaacgcg atccacaagt tgccgggctg aaagtgcctc cgcactcgat cgaagcggag
     3901 cagtcggtgt tgggcggttt aatgctggat aacgaacgct gggacgatgt agccgagcgt
     3961 gtggtggcag acgattttta cacccgccca caccgccata tctttactga aatggcgcgt
     4021 ttgcaggaaa gcggtagtcc tatcgatctg attacccttg cggaatcgct ggaacgccag
     4081 gggcaactcg atagcgtcgg tggttttgct tatctggcag agctgtcaaa aaatacgcca
     4141 agtgcggcga acatcagtgc ttatgctgac atcgtgcgtg aacgtgccgt tgtccgtgag
     4201 atgatctcgg ttgcgaatga gattgctgaa gccggttttg atccgcaggg gcgtaccagc
     4261 gaagatctgc tggaccttgc tgaatcccgc gtctttaaaa ttgccgaaag tcgtgcaaac
     4321 aaagacgaag ggccgaagaa catcgccgat gtgctcgacg caaccgtggc gcgtattgag
     4381 cagttgtttc agcagccaca cgatggcgtt accggagtaa acaccggtta tgacgatctc
     4441 aacaaaaaaa ccgctggctt gcagccgtcg gatttgatca tcgtcgccgc gcgtccgtcg
     4501 atgggtaaaa caacatttgc gatgaacctc gtcgaaaacg cggcgatgtt gcaggataaa
     4561 ccagtactta tcttctcgct ggagatgcct tcagaacaga ttatgatgcg ttctctggcg
     4621 tcgctgtcgc gcgttgacca gactaaaatc cgtaccgggc agctcgatga tgaagactgg
     4681 gcgcgcattt ccggcaccat gggtattttg ctcgaaaaac gcaatatcta tatcgatgac
     4741 tcctccggcc tgacgccaac ggaagtgcgt tcccgcgcac gccgtattgc ccgtgaacac
     4801 ggcggcatcg ggcttatcat gatcgactac ctgcaactga tgcgcgtacc ggcgctttcc
     4861 gataaccgta cgctggaaat tgcagaaatc tctcgctcgc tgaaagcact ggcgaaagaa
     4921 ctgaacgtgc cggtggtggc gctgtcccag ttgaaccgtt ctctggaaca acgtgccgac
     4981 aaacgcccgg tcaactccga cctgcgtgaa tctggctcta tcgagcagga tgcggacttg
     5041 attatgttta tctatcgtga tgaggtgtat cacgaaaaca gtgatttaaa aggcatcgcg
     5101 gaaattatta tcggtaaaca acgtaacggc ccaatcggga cggtacgcct gacctttaac
     5161 ggtcaatggt cgcgcttcga caactatgcg gggccgcagt acgacgacga ataataatta
     5221 ttttatgaat taggtaatta aagcaaacac ttatcaagga acacaaatgc aagcggcaac
     5281 tgttgtgatt aaccgccgcg ctctgcgaca caacctgcaa cgtcttcgtg aactggcccc
     5341 tgccagtaaa atggttgcgg tggtgaaagc gaacgcttat ggtcacggtc ttcttgagac
     5401 cgcgcgaacg ctccccgatg ctgacgcctt tggcgtagcc cgtctcgaag aagctctgcg
     5461 actgcgtgcg gggggaatca ccaaacctgt actgttactc gaaggctttt ttgatgccag
     5521 agatctgccg acgatttctg cgcaacattt tcataccgcc gtgcataacg aagaacagct
     5581 ggctgcgctg gaagaggcta gcctggacga gccggttacc gtctggatga aactcgatac
     5641 cggtatgcac cgtctgggcg taaggccgga acaggctgag gcgttttatc atcgcctgac
     5701 acagtgcaaa aacgttcgtc agccggtgaa tatcgtcagc cattttgcgc gcgcggatga
     5761 accaaaatgt ggcgcaaccg agaaacaact cgctatcttt aatacctttt gcgaaggcaa
     5821 acctggtcaa cgttccattg ccgcgtcggg tggcattctg ctgtggccac aatcgcattt
     5881 tgactgggtg cgcccgggca tcattcttta tggcgtctcg ccgctggaag atcgctccat
     5941 cggtgccgat tttggctgtc agccagtgat gtcactaacc tccagcctga ttgccgtgcg
     6001 tgagcataaa gccggagagc ctgttggtta tggtggaacc tgggtaagcg aacgtgatac
     6061 ccgtcttggc gtagtcgcga tgggctatgg cgatggttat ccgcgcgccg ctccatccgg
     6121 tacgccagtg ctggtgaacg gtcgcgaagt accgattgtc ggacgcgtgg cgatggatat
     6181 gatctgcgta gacttaggtc cacaggcgca ggacaaagcc ggggatccgg tcattttatg
     6241 gggcgaaggt ttgcccgtag aacgtatcgc tgaaatgaca aaagtgagcg cttacgaact
     6301 tattacgcgc ctgacttcaa gggtcgcgat gaaatacgtg gattaatcgt tctgtaatat
     6361 ttgattgtct gtgccggatg cggcgtgaat gccttatccg gccaataaaa tcctaaaaat
     6421 tcaataagtt gatgttcttt catgctctta taaaggtcgt gcctctggcg gatgttcgtt
     6481 tgtcatgagt ctcactctgt tgctaattgc cgttcgctcc tgaacatcca ctcgatcttc
     6541 gccttcttcc ggtttattgt gttttaacca cctgcccgta aacctggaga accatcgcgt
     6601 gtttcaaaaa gttgacgcct acgctggcga cccgattctt acgcttatgg agcgttttaa
     6661 agaagaccct cgcagcgaca aagtgaattt aagtatcggt ctgtactaca acgaagacgg
     6721 aattattcca caactgaaag ccgtggcgga ggcggaagcg cgcctgaatg cgcagcccca
     6781 tggcgcttcg ctttatttac cgatggaagg gcttaacagc tatcgccatg ccattgcgcc
     6841 gctgctgttt ggtgcggacc atccggtact gcaacaacag cgcgtagcaa ccattcaaac
     6901 ccttggcggc tcaggggcat tgaaagtggg cgcggatttc ctgaaacgct acttcccgga
     6961 atcaggcgtc tgggtcagcg atcctacctg ggaaaaccac gtagcaatat tcgccggggc
     7021 tggattcgaa gtgagtactt acccctggta tgacgaagcg actaacagcg tgcgctttaa
     7081 tgacctgttg gcgacgctga aaacattacc tgcccgcagt attgtgttgc tgcatccatg
     7141 ttgccacaac ccaacgggtg ccgatctcac taatgatcag tgggatgcgg tgattgaaat
     7201 tctcaaagcc cgcgagctta tcccattcct cgatattgcc tatcaaggat ttagtgccgg
     7261 tatggaagag gatgcctacg ctattcgcgc cattgccagc gctggattac ccgctctggt
     7321 gagcaattcg ttctcgaaaa ttttctccct ttacggcgag cgcgtcggcg gactttctgt
     7381 tctgtgtgaa gatgccgaag ccgctggccg cgtactgggg caattgaaag caacagttcg
     7441 ccgcaactac tccagcccgc cgaattttgg tgcgcaggtg gtggctgcag tgctgaatga
     7501 cgaggcattg aaagccagct ggctggcgga agtagaagag atgcgtactc gcattctggc
     7561 aatgcgtcag gaattggtga aggtattaag cacagagatg ccagaacgca atttcgatta
     7621 tctgcttaat cagcgcggca tgttcagtta taccggttta agtgccgctc aggttgaccg
     7681 actacgtgaa gaatttggtg tctatctcat cgccagcggt cgcatgtgtg tcgccgggtt
     7741 aaatacggca aatgtacaac gtgtggcaaa ggcgtttgct gcggtgatgt aatgcaggaa
     7801 agcaggctgg agttacccag cctgca
//
LOCUS       DAZPAC010000191         7823 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZPAC010000191 DAZPAC010000000
VERSION     DAZPAC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.191"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            78..2393
                     /gene="bisC"
                     /locus_tag="ACHUPM_003456"
     CDS             78..2393
                     /gene="bisC"
                     /locus_tag="ACHUPM_003456"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR0871139.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2362..2802)
                     /locus_tag="ACHUPM_003457"
     CDS             complement(2362..2802)
                     /locus_tag="ACHUPM_003457"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0871140.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2799..3362)
                     /gene="tag"
                     /locus_tag="ACHUPM_003458"
     CDS             complement(2799..3362)
                     /gene="tag"
                     /locus_tag="ACHUPM_003458"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR0871141.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3520..4218
                     /locus_tag="ACHUPM_003459"
     CDS             3520..4218
                     /locus_tag="ACHUPM_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR0871142.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4447..5655
                     /locus_tag="ACHUPM_003460"
     CDS             4447..5655
                     /locus_tag="ACHUPM_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0871143.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5979..7670
                     /gene="eptB"
                     /locus_tag="ACHUPM_003461"
     CDS             5979..7670
                     /gene="eptB"
                     /locus_tag="ACHUPM_003461"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR0871144.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
BASE COUNT         1792 a         1919 c         2077 g         2035 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttacgc ggcgttctgc ggtgctctct tccatgcagg
     1201 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     1261 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     1321 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     1381 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     1441 cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
     1501 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     1561 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     1621 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     1681 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     1741 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     1801 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     1861 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     1921 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     1981 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     2041 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     2101 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     2161 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     2221 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     2281 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     2341 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     2401 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     2461 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     2521 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     2581 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     2641 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     2701 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     2761 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
     2821 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     2881 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ctttagatag ggcgtcggag
     2941 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     3001 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     3061 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     3121 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     3181 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     3241 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     3301 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     3361 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     3421 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     3481 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     3541 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     3601 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     3661 tacacctggg atagtgatca tcaacctaat tacaacgata ttttgtcgca acgtattcaa
     3721 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     3781 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     3841 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     3901 actaccacgc agaccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt
     3961 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     4021 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     4081 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     4141 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     4201 gttagcgcca gattttaacg taacagtcac aattgaaacc attaaataac aataattgtg
     4261 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     4321 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc
     4381 ataaagtgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     4441 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     4501 attacccagt ttgcgctggg gtcggtttat acctggagcc tgtttaatgg cgcgctttcc
     4561 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     4621 ctggcaattt cgtcttctgt tgcgggcaaa ttacaggaac gtttttgcgt taaacgcgtc
     4681 accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     4741 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     4801 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     4861 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     4921 ctcgaaacgg tgggtctgga aaaaacattt gtgatttggg gagcgattgc gctggtgatg
     4981 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     5041 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     5101 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     5161 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
     5221 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
     5281 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
     5341 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
     5401 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
     5461 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
     5521 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
     5581 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
     5641 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
     5701 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
     5761 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
     5821 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
     5881 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgttaa
     5941 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
     6001 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
     6061 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
     6121 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
     6181 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
     6241 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
     6301 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
     6361 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
     6421 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg
     6481 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
     6541 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
     6601 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
     6661 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgaga
     6721 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
     6781 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
     6841 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
     6901 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
     6961 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
     7021 tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
     7081 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
     7141 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
     7201 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
     7261 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
     7321 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
     7381 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
     7441 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
     7501 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
     7561 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
     7621 acaacaactg gtgtcacatc ccgcagacaa aagaggcagc ggctaactaa gaggcctgct
     7681 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     7741 cagtaagatg cgccccgcat tcggtgattg gcgcagcttg gtagcgcact tcgttcggga
     7801 cgaaggggtc ggaggttcga atc
//
LOCUS       DAZPAC010000192         7819 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZPAC010000192 DAZPAC010000000
VERSION     DAZPAC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.192"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            36..1190
                     /locus_tag="ACHUPM_003462"
     CDS             36..1190
                     /locus_tag="ACHUPM_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR0871145.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKAITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            1341..2165
                     /gene="traE"
                     /locus_tag="ACHUPM_003463"
     CDS             1341..2165
                     /gene="traE"
                     /locus_tag="ACHUPM_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFR0871146.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLINDSSNTEVIFWHSFPFAGNTKREYCGVILRQEKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            2251..3453
                     /locus_tag="ACHUPM_003464"
     CDS             2251..3453
                     /locus_tag="ACHUPM_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFR0871147.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDVDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            3513..4097
                     /locus_tag="ACHUPM_003465"
     CDS             3513..4097
                     /locus_tag="ACHUPM_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFR0871148.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            4491..4949
                     /gene="traH"
                     /locus_tag="ACHUPM_003466"
     CDS             4491..4949
                     /gene="traH"
                     /locus_tag="ACHUPM_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HFR0871149.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            4946..5764
                     /gene="traI"
                     /locus_tag="ACHUPM_003467"
     CDS             4946..5764
                     /gene="traI"
                     /locus_tag="ACHUPM_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HFR0871150.1"
                     /translation="MKALTSLILPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLISTEGGSR"
     gene            5761..6909
                     /gene="traJ"
                     /locus_tag="ACHUPM_003468"
     CDS             5761..6909
                     /gene="traJ"
                     /locus_tag="ACHUPM_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFR0871151.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPNLLEAMVCKSGLG
                     FVCGETGSGKSTLCAALYCYIKKNYPNAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VASFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            6906..7196
                     /locus_tag="ACHUPM_003469"
     CDS             6906..7196
                     /locus_tag="ACHUPM_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR0871152.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            7211..7762
                     /locus_tag="ACHUPM_003470"
     CDS             7211..7762
                     /locus_tag="ACHUPM_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFR0871153.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWRP"
BASE COUNT         2129 a         1925 c         1806 g         1959 t
ORIGIN      
        1 tgttggcatt attttttgcc tcttttgagg ttagcatgcc gtctccacgc atccgtaaaa
       61 tgtccctgtc acgcgcactg gataagtacc tgaaaacagt ttctgttcac aagaaagggc
      121 atcaacagga gttttaccgg agcaatgtta tcaagcgata tcccattgct cttcggaata
      181 tggacgaaat aacaaccgtt gatattgcta catacagaga cgttcgttta gcagaaataa
      241 acccccgaac gggtaaagcc attacaggta atactgtacg tcttgaactc gcccttctgt
      301 catctctgtt caatattgct cgtgttgaat ggggaacctg tcgtactaac ccggttgaac
      361 tggttcgcaa gccgaaagta tcctccggac gagatcgccg gctaacgtct tcagaagaac
      421 gtcgcctttc tcgctatttc cgcgaaaaaa atctgatgtt gtatgtcatt ttccatcttg
      481 cccttgaaac agccatgcgg cagggcgaaa tactggcctt acgttgggag cacattgatt
      541 tgcgccacgg tgtggctcat ttacctgaaa ccaaaaacgg tcactcacgg gatgttcctc
      601 tgtccagacg tgcccgtaac tttcttcaaa tgatgcccgt taatctccac ggcaatgttt
      661 ttgattacac cgcatccggc tttaaaaatg cctggagaat agccacacaa cgacttcgca
      721 tcgaggacct gcattttcac gatctacggc atgaagcaat aagccgcttc ttcgaactgg
      781 gtagcctgaa tgtaatggag attgctgcaa tatcaggaca tcgttccatg aatatgctga
      841 aacggtatac tcatcttcgt gcatggcaac tggtcagtaa gcttgatgcc cgccggcggc
      901 agacacaaaa agtggcagca tggtttgtgc cgtatcctgc ccatatcacg actatcgatg
      961 aagaaaatgg gcagaaagcg catcgtattg agatcggtga ttttgataac cttcacgtca
     1021 ctgccacaac aaaagaggaa gcagttcacc gcgccagtga ggttttgttg cgtacactgg
     1081 ccattgcagc acagaaaggc gaacgtgtcc catctcccgg agcgttacct gttaacgacc
     1141 ctgactacat tatgatttgc cctctgaacc cgggcagcac accgctgtaa acatcattca
     1201 aaacacatac agaaaggcga gttacaacaa ctcgccaaac acattcgatc tgcatgatcg
     1261 aaattattcg tttcaccaga aaaaacaccg aagatacaat aaccacatga gctgaaaaga
     1321 ctttttcagt cggaggaaca atgagactga atacgaccgg tattgcggcc cgaatgatgc
     1381 tctcgctgga caaaaaagca atcggcgaaa aactcatcaa cggtcgtcag ataaacccga
     1441 caccactgca ggtccgttac ccacaagggg taagagaagt tctggggata atgagcgaac
     1501 aattagccat atccaccgct gatttaaccc gaatcctgct tgaggatgct cttcataata
     1561 tgtttatgcc tgcggataac accacaggaa acataataag cagaattgaa tacatcatgc
     1621 tgtcgcatga tataaatgcg ccattactgg caacattgct gtcgccatgg aatatccgct
     1681 ccacggttat acaggacccg gccagacttg cagactatct ttcagctgac gctctcgaac
     1741 atcttgctga atgttttaat ctcaatccgg actggctgaa tggccatgaa aactatccga
     1801 tagcgttgtc cggtgaatgg ccagatactg ctgataattt cagaatgctt attaatgata
     1861 gcagcaacac tgaagttatt ttctggcata gtttcccctt cgcaggaaac acgaaaagag
     1921 aatattgcgg ggtaatattg agacaggaaa aagaaataaa tggctctgta atatatccgg
     1981 cactatcatt atcaccaaca cttctgaacg atgaaaaaag aaaatggtta actgaatata
     2041 caaccaggca gaatacaaca atgtctttgc gacgcgtcac gttgagacct ggccttgccg
     2101 ggaaccttat tacagggcaa atactacctg ttagcttatt taatacgtct ttattaccct
     2161 ggtaaatacc agatagtacc aaagtaaaaa attacggcac atccgtgtca tagcgttgtt
     2221 ttgtctaaat tctgataaac aggaaattaa atgatgaaga aaaatcatat tacccgcacc
     2281 atcattgcat ctgctgtatt attttccttt aacgctgcag cagcgacgtc atattttgaa
     2341 gcgcgtaacg atgcaatggg tggcaccggg gtagcatctt cccattatgg tgtggcacct
     2401 ctggcaaacc cggctttact gacaaagcac aacagcaatg atgatttcag ccttcttctg
     2461 ccgtctgttg gggcacaagt tgctgatcct gatgatgtat caaataaagc tgatgatgtt
     2521 aaagatgact gggatttgtt tgacagtgct gtagataacc agcatggcgt acaacaggca
     2581 gcggcaaatc ttaaacatcg tctgcaggaa ttccgcaata tcaatgcaga tgctcaggta
     2641 ggtgtatcag ctgtagcagc catggcaaat gacactctgc cgtttgccct tatggtcaag
     2701 tcttacggaa ctgtcagcgt aaacggaaaa gtaaacgatg cagacctgga ttatctggac
     2761 aaagttgcca atggaacaat taccgacgtc gataagaatg ccctgacctc acgcgcattc
     2821 gggcgtgcag ctgtaattac cgacgtcgga atttcatttg ccaaagaact ggaaacggcc
     2881 ggacaaaaat ggtctcttgg cgtcacacca aaatatcagc gtgtggacct gtttaactac
     2941 aacgtaacgg ttcgtgatta cgacaaagac gattttgacg gtgacaagta ccacaacacg
     3001 aaaaacggat tcaacgccga tattggtgct tatacagacc tgaatgacaa ctggactgtc
     3061 ggtcttgttg cccagaacat cattccacgc agtattgata ctaaagtcgt caatggcttt
     3121 aaagaaacct tcaaagttcg tccacaggca accgccggtg tatcctggca taatgacctg
     3181 tttaccactg ctcttgacgt tgatctgact cctgccagtg gcttcacttc tgacagcaaa
     3241 cgtcagtttg caagtgttgg tgctgaattt aacgcatgga aatgggcgca attgcgtgcc
     3301 ggttatcgtc agaacatggc atcaaactcc ggaagcgcct tcaccgctgg cgttggtatc
     3361 tctccatttg acgtcgtaca tatcgatgtt tccggcctgg taggaactga ccacgattac
     3421 ggtgcaatgg cacaacttca gttcactttc tgattacagt ccccgccccc ggcactaaac
     3481 ccgggggtat ttttatattc agtttaaata acatgatgaa accacgttct tcttattcaa
     3541 agacagcatt cattctttta ttttctgttt tcctggtagc tgcagtgaca aaagcaaaaa
     3601 gctcgcttcc cgatatcacc ctggaacagg caaaagaaat taatgctgat aacaccgtta
     3661 ttttcctttt ccgccatgga gaacgttgcg accgttcaga tatgccctgc tattcggata
     3721 agagcggaat cactattacc ggtacagaga aagcacagca ggaaggtatt aagttcgcaa
     3781 caatcttcag tgaatatgat atttactcca gcaatgcggt aagaaccata caaactgcaa
     3841 aattcttctc cggtaaagaa cccgtcgtta tggacagttt atcagactgc aataatgatc
     3901 tttataagac gcttgaatca atcgcaagag aatctcataa aaggaacata gtaataatga
     3961 cacataatca ttgtttatca tttctggcca gagataggct cggcaagaaa ttcaaaccgg
     4021 cgtaccttga tgccctgatc atgcattacg atggtacccg gctcatactt gacggaaaat
     4081 acaacaaaga ggcatagaga tatttgaatt tacataaatt ctgatatact cagtaagata
     4141 caagagtgta cgggcacatg tgcctgtctg aattgcaggt tatccggacg agggccggga
     4201 actgtttcag gtacaagcgc cggtacgttc gattcataaa tcgtcgagtc atccatcatg
     4261 gatcgattcg gcgcggagcg ttaaagatta cgctcacatt catattatgt ctgacgacca
     4321 atcaaccagc cagtatcagg aataatcacc ttcagtaaac tatgaggcta tttacctggc
     4381 cgcactggca tttatttccg gtaacgcaag tatagccgga aacaaacatc atcctgtctg
     4441 taccggagca ccggtatatc ccattctgat ttaactgctc aggaattatt atgaaccgta
     4501 ttatacctgc ggggtgtctt gccgcccttt ttctggctgg ctgcaactcc ccttctcagc
     4561 atactcctgt tccgcgtaac tacggtgctg cctctgatgt ggcggttacc cggcacactc
     4621 agtacacatt atgggacaaa ggtgtgtttt ccggccaact tccggtttca acggggtctc
     4681 atcttctgac agctaacagc gatcgccttt cctttgactg ggaaggtgat gcgattgaat
     4741 tactgaacga actggccaga gtcaggggga tgcagtttaa ctacaatggt gtccgcctgc
     4801 cgttaccagt caaccttcac gtccgggata tgacgttttc caatacgttg agacttattg
     4861 aagcccagac agcctggaga gcaacaatcc atcaatatcc gggcttgtta caggtcagct
     4921 tcatgcagcc ggagaaccgg aaaaaatgaa agcactcacc tctctcattt tgccagtaat
     4981 actgcttact ggttgttctt cttcccctac catctcgcca ggtggtgtca atacgccacc
     5041 acctgacatt aatgcatggc ttaaaccacg aagtgaaaag ccggatagtc tgtcagaaac
     5101 acgctggaaa atgctgaccg acgcaggtaa aacgcttgga tttcgtggtg gcaaggctca
     5161 gcgctcatgg gagctgattc aggcactcaa cgcccgggaa agcacactta atgccttata
     5221 cgatttccgg ccactgatta gcccggaagg ctggctacca ccggttattg atgaagcaca
     5281 ggacgtagct catatcacgc cagatcaaat ccggacctct tcccgggtct ggaccatcat
     5341 tcgccctgaa cgctttgtca gcaatccacc gggctggcgt gactggcttc tccgggggct
     5401 ttcaacaaca gccactccgg gtacagaagg cagcgtcgtt ccagaagaca gcgtgcaacg
     5461 aaaagtgtgg gaaaccgcat tacggcaggg atggcaggaa ggaagacaaa acgccgacct
     5521 gacgcttgaa gccaaccaga aaaccctcac ccgtgattac cggggcatga tgctgtactc
     5581 acttctctgg cgtcagggca tgatcacccg tccggatgtt tctgatcaaa tgcaaaccgt
     5641 tacaggtgac gggaaaaaac tggtcactgg agatcgtgtt cgtcgcctta aaaatcatgc
     5701 ggaattcaat cttcagaaat ctcactggcg tccgcttata agcactgaag gaggctcccg
     5761 atgaatattc ctgatgagtt tggtcttttt ccgtttcagc gtttcacagc agatgaacta
     5821 cggcatttct ttgtctggtg tgccgcacat aaagtcagtg atgtggatct gaccggtggg
     5881 tcaccagttt ctgtcagtcg ctttggccga cgggtgcgtt gttccagtgc aacgttacca
     5941 accacactca tgtcctccct gattgatgaa ctgttcggtc gggaagtcat tccccgtgtt
     6001 ctggcaggca atccggttga caggacgata cagattaacg gggatgcatc cggtcgctat
     6061 ggtcttaaac gcggagaacg catccggtta cgctctcacc tcattcaggg cacatctggt
     6121 gcggaggaga aagcaatatc aataaccatg cgtgtcatcc cgacagaaat accggacatc
     6181 ctctccatga acattgaacc aaacctgctt gaagcaatgg tctgcaaaag cggtctggga
     6241 tttgtatgtg gtgaaacagg gtcaggaaaa tcaacgctgt gtgcagcact gtactgttac
     6301 attaagaaga attatcccaa cgccaaaatc gtgacatatg aagacccggt ggaatatatc
     6361 ctgggaaatg aaaacgatct gctcccaccg catcaggcag aaatcgggcg tgatgtggcc
     6421 agttttgctg ccggactgcg ttctgctgta cgacgtaatc ccgaaattat cggtgtcggt
     6481 gaaatcaggg ataacgaaac cgccgacgca gcggtacagg caggcaacac cgggcattac
     6541 tgcctgagca ccatgcatac gaaatctcct ggtgaaacgc tggcacgtct tcttgggctg
     6601 tttccccctg tcatcaggga ttcgatggcc tgggctgtcc tgtcactgtt gcagttcatt
     6661 ctggttcagg ttctggtcag aacaaatgac ggtggtcgta aagccgtccg ggaatatatc
     6721 gtcattaacg atgagcttcg cgacaatctt tccggaatgc cacatgcaga gtgggggcat
     6781 cacattgatg ccataatccg gcaggagaag cgccgtatcc gggaccagat tctggaaatg
     6841 tatatccgga atgaagttga ccgaagagaa gcgattcttt ttatcccgcc aggggagtta
     6901 cggtcatgaa aagagaaggt ttctggcgat atgcaacacc gccagtcaac atccagggcg
     6961 tacctctgcc gttcaccatg atttatctgt ttatatgccc ctttccctcc aaaacaacct
     7021 tctggatttg caccggcatc attttatttt tcgttattct cgaccgttca ggctggactg
     7081 tcaggacgat ctgtactcgc attttcagca ttctgagagg ggccatcgcc tccggtcgtc
     7141 catggtggta tcgccaccat acggaatcac cacgagactg gactggcctc gattaaattc
     7201 tggagtcttt atgtcaggta aaaaaactat cgttactctg ctccgggtat cgctactggc
     7261 atgcccgtta ctgttcacga caccatcgtt tgcaatgatc gacacgccat ccgtgaaggt
     7321 cggcttttct cctgaaggaa gcgcatccgc tctggtgctt gacacaatca acagtgcgga
     7381 aagctccatc aggatgatgg catattcctt caccgacccg gatgtaatgc atgcgctggc
     7441 aaaagcgaaa aaacgcggag tggacgtccg tattgttgtt gatgacaagg ggaatacgaa
     7501 ccgggcaagt caggaagcga tgaaatatat caacctgctg gacattcctc tccggactgt
     7561 agatgccttc cccatccatc acgacaaagt catcattgtt gacggaaaca cggttgaaac
     7621 tggctcctat aacttctctc gtgctgctgc acgcaagaat tcagagaatg tcgttgtgct
     7681 caagaatatg ccggatgtag ccgcacaata tcttgaacac tggcaggatc gctggaataa
     7741 gggtacagac tggagaccct gacaaacatt tctcccccga ttttctatcc ccccagccac
     7801 ccccggcttc cggcggtgg
//
LOCUS       DAZPAC010000193         7789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZPAC010000193 DAZPAC010000000
VERSION     DAZPAC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.193"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            253..756
                     /gene="dps"
                     /locus_tag="ACHUPM_003471"
                     /gene_synonym="pexB"
     CDS             253..756
                     /gene="dps"
                     /locus_tag="ACHUPM_003471"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR0871154.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1160..1906
                     /gene="glnH"
                     /locus_tag="ACHUPM_003472"
     CDS             1160..1906
                     /gene="glnH"
                     /locus_tag="ACHUPM_003472"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR0871155.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            2045..2704
                     /gene="glnP"
                     /locus_tag="ACHUPM_003473"
     CDS             2045..2704
                     /gene="glnP"
                     /locus_tag="ACHUPM_003473"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR0871156.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2701..3423
                     /gene="glnQ"
                     /locus_tag="ACHUPM_003474"
     CDS             2701..3423
                     /gene="glnQ"
                     /locus_tag="ACHUPM_003474"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR0871157.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3540..5765
                     /gene="ybiO"
                     /locus_tag="ACHUPM_003475"
     CDS             3540..5765
                     /gene="ybiO"
                     /locus_tag="ACHUPM_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFR0871158.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(5762..6688)
                     /gene="rlmF"
                     /locus_tag="ACHUPM_003476"
     CDS             complement(5762..6688)
                     /gene="rlmF"
                     /locus_tag="ACHUPM_003476"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR0871159.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6964..7224
                     /gene="mcbA"
                     /locus_tag="ACHUPM_003477"
     CDS             6964..7224
                     /gene="mcbA"
                     /locus_tag="ACHUPM_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR0871160.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7489..>7789
                     /locus_tag="ACHUPM_003478"
     CDS             7489..>7789
                     /locus_tag="ACHUPM_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0871161.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         1893 a         1985 c         1947 g         1964 t
ORIGIN      
        1 tacttttcct ctacaccgtc tttatatatc gaattatgca aaagcatatt tattccgaaa
       61 attcctggcg agcagataaa taagaattgt tcttatcaat atatctaact cattgaatct
      121 ttattagttt tgtttttcac gcttgttacc actattagtg tgataggaac agccagaata
      181 gcggaacaca tagccggtgc tatacttagt ctcgttaatt actgggacat aacatcaaga
      241 ggatatgaaa ttatgagtac cgctaaatta gttaaatcaa aagcgaccaa tctgctttat
      301 acccgcaacg atgtctccga cagcgagaaa aaagcaacag tagagttgct gaatcgccag
      361 gttatccagt ttattgatct ttctttgatt accaaacaag cgcactggaa catgcgcggc
      421 gctaacttca ttgccgtaca tgaaatgctg gatggcttcc gcaccgcact gatcgatcat
      481 ctggatacca tggcagaacg tgcagtgcag ctgggcggtg tagctctggg gaccactcaa
      541 gttatcaaca gcaaaacccc gctgaaaagt tacccgctgg acatccacaa cgttcaggat
      601 cacctgaaag aactggctga ccgttacgca atcgtcgcta atgacgtacg caaagcgatt
      661 ggcgaagcga aagatgacga caccgcagat atcctgaccg ccgcgtctcg cgacctggat
      721 aaattcctgt ggtttatcga gtctaacatc gaataaatcc atcgctgatg gtgcagaact
      781 ttagtacccg ataaaagcgg cttcctgaca ggaggccgtt ttgttttgca gcccacctca
      841 acgcacttat ttagtgcatc catctgctat ctccagctga ttaagtaaat tttttgtatc
      901 cacatcatca cacaatcgtt acataaagat tgttttttca tcaggtttta cgctaaataa
      961 tcactgtgtt gagtgcacaa ttttagcgca ccagattggt gccccagaat ggtgcatctt
     1021 cagggtattg ccctataaat cgtgcatcac gtttttgccg catctcgaaa aatcaaggag
     1081 ttgcaaaact ggcacgattt tttcatatat gtgaatgtca cgcaggggat cgtcccgtgg
     1141 atagaaaaaa ggaaatgcta tgaagtctgt attaaaagtt tcactggctg cactgaccct
     1201 ggcttttgcg gtttcttctc atgccgcgga taaaaaatta gttgtcgcga cggataccgc
     1261 cttcgttccg tttgaattta aacagggcga taaatatgtg ggctttgacg ttgatctgtg
     1321 ggctgccatc gctaaagagc tgaagctgga ttacgaactg aagccgatgg atttcagtgg
     1381 gatcattccg gcactgcaaa ccaaaaacgt cgatctggcg ctggcgggca ttaccatcac
     1441 cgacgagcgt aaaaaagcga tcgatttctc tgacggctac tacaaaagcg gcctgttagt
     1501 gatggtgaaa gctaacaata acgatgtgaa aagcgtgaaa gatctcgacg ggaaagtggt
     1561 tgctgttaag agcggtactg gctccgttga ttacgcgaaa gcaaacatca aaactaaaga
     1621 tctgcgtcag ttcccgaaca tcgataacgc ctatatggaa ctgggcacca accgcgcaga
     1681 cgccgttctg cacgatacgc caaacattct gtacttcatc aaaaccgccg gtaacggtca
     1741 gttcaaagcg gtaggtgact ctctggaagc gcagcaatac ggtattgcgt tcccgaaagg
     1801 tagcgacgag ctgcgtgaca aagtcaacgg cgcgttgaaa accctgcgcg agaacggaac
     1861 ttacaacgaa atctacaaaa aatggttcgg tactgaaccg aaataataac gctacacctg
     1921 taaagcgcac tggcagttcc ctctcccctc tggggagagg attagggtga ggggcgcaaa
     1981 cccgctccgg ggccattaat taccctgaat ttgattattt acaccacggt aacaggaaca
     2041 acatatgcag tttgactgga gtgccatctg gcctgccatt ccgcttctga ttgaaggtgc
     2101 caaaatgacc ctgtggattt cggtcctcgg tctggctggc ggtctggtaa tcggattgct
     2161 ggcaggtttt gcacgcacct tcggtggttg gatagccaac cacgtcgcgc tggtctttat
     2221 tgaggtgatc cgcggcacac ctatcgtcgt ccaggtgatg tttatttatt tcgccctgcc
     2281 gatggcgttt aacgacttac gcatcgaccc atttactgcg gcggtggtca ccatcatgat
     2341 caactccggc gcgtacattg cggaaatcac gcgtggtgcg gtgctgtcta ttcacaaagg
     2401 ttttcgtgaa gcaggactgg cgctcggtct ttcacgttgg gaaaccattc gctacgtcat
     2461 tttaccgctg gcactgcgtc gtatgctgcc gccgctgggt aaccagtgga tcatcagcat
     2521 taaagacacc tcgctgttta ttgtgatcgg cgtggcggaa ctgacccgtc aggggcaaga
     2581 aattattgcc ggtaacttcc gcgcccttga gatctggagc gccgtggcgg tgttctatct
     2641 gattattacc ctggtgctaa gctttatttt gcgtcgtctg gaaagaagga tgaaaatcct
     2701 gtgattgaat ttaaaaacgt ctccaagcac tttggcccaa cccaggtgct tcacaatatc
     2761 gatttgaaca ttgcccaggg cgaagtcgtg gtgattatcg ggccgtccgg ttccggtaaa
     2821 tcgaccctgc tgcgctgcat caacaaactg gaagaaatca cctccggcga tctgattgtc
     2881 gatggcctga aggttaacga tccgaaagtt gacgagcgcc tgattcgcca ggaagcaggt
     2941 atggtgttcc agcagtttta cctcttcccg catctgacgg cgctggaaaa cgtcatgttt
     3001 ggtccgctac gcgtgcgtgg cgcgaacaaa gaagaggcgg aaaaactggc gcgtgagctg
     3061 ctggcgaaag tcggtctggc agaacgtgca catcactacc cttccgaact ttctggtggt
     3121 caacagcagc gtgtggcgat tgcccgcgcg ctggcggtga agccgaaaat gatgctgttt
     3181 gatgaaccga cttccgctct tgacccggaa ctgcgccatg aagtgctgaa ggttatgcag
     3241 gatctggctg aagaagggat gacgatggtg atcgtgaccc acgaaatcgg ttttgccgag
     3301 aaagtagctt cgcggctgat ctttatcgac aaaggccgga ttgcggaaga tggcgatccg
     3361 caggtattga tcaagaaccc gccgagccag cgcttgcagg aatttttgca gcacgtctct
     3421 taacttctct gcctcccttt cacccgaaag ggaggcacac cagattcctc tcatttaaaa
     3481 tcgcccctcc cccagcatct atacttatct ttttgctctg ttttctcact ggaggagtca
     3541 tgcggtggat cctgttcatc ctcttctgcc tgctgggcgc acctgcccac gcggtatcca
     3601 tacccggcgt tacaaccaca acgacaacgg actcaacgac tgaaccggcc ccggaaccgg
     3661 atatcgaaca aaaaaaagcg gcctatggcg cactggcgga tgtgctggat aacgacacct
     3721 cgcgtaaaga attgatcgac cagttgcgta ctgtcgccgc gacgccccct gcggaaccgg
     3781 tcccgaagat cgtgccgccg acgctggttg aagagcaaac cgtgctgcaa aaggtcaccg
     3841 aagtcagccg ccattatggt gaagcccttt ccgcccgctt cgggcaactt tatcgcaata
     3901 tcaccggctc cccgcataag ccatttaatc cacaaacctt cagcaatgcg ctgagccatt
     3961 tttcaatgtt agcggtatta gtgtttggtt tttactggct gattcgcctg tgcgcactgc
     4021 cgctgtatcg caaaatgggt cagtgggcga ggcaaaaaaa tcgtgagcgc agcaactggt
     4081 tgcagcttcc ggcgatgatt atcggggcgt ttattatcga cctgctgtta ctggccctga
     4141 cattatttgt cggccaggta ttaagcgaca acctgaatgc aggcagtcgc accatcgctt
     4201 tccaacaaag tttgtttctc aacgcctttg ccctgattga atttttcaaa gccgtactac
     4261 gcctgatttt ttgcccaaac gtggcggagc tgcgcccgtt cacgattcag gacgagagcg
     4321 cccgttactg gagtcgtcgc ctgagctggt taagcagttt gataggttat ggcctgattg
     4381 tggccgtgcc gattatctct aatcaggtga atgtacagat aggtgcgctg gcgaacgtca
     4441 tcattatgct gtgcatgacc gtctgggcgt tgtacctgat ctttcgtaat aaaaaagaga
     4501 ttacccagca tttgctcaac tttgcggagc attcgctggc ctttttcagc ctgtttatcc
     4561 gcgcctttgc gctggtgtgg cactggctgg caagcgccta ttttatcgtg ctgtttttct
     4621 tttcgttgtt cgatccgggc aacagcctga aatttatgat gggtgcaacg gtgcgcagcc
     4681 tggcgattat tggtatcgca gcgtttgttt ccggtatgtt ttcccgctgg ctggcaaaaa
     4741 ccatcactct ctcgccacat actcagcgta actatccaga gttgcaaaaa cggctgaatg
     4801 gctggctttc ggcggcgctg aaaacggcgc gtattctgac cgtctgcgtg gcggtaatgt
     4861 tgctgttgag cgcatgggga ttgttcgatt tctggaactg gctgcaaaac ggcgcggggc
     4921 agaaaaccgt agatatcctg atccgtatcg cacttattct ttttttctcg gcggttggct
     4981 ggacggtgct cgccagtttg atcgaaaacc ggctggcttc ggatattcat ggccgcccgc
     5041 tacccagcgc ccgtacgcgt accctgctga cgctgttccg taacgcgctg gcggtgatta
     5101 tcagtaccat caccatcatg attgtgttgt cggaaatcgg cgtcaatatc gcgccattgc
     5161 tggcaggtgc cggggcatta ggtctggcta tctcgtttgg ttcgcaaacg ctggtgaaag
     5221 atatcattac cggggtgttt attcagtttg aaaacggcat gaacactgga gatttggtga
     5281 ctatcgggcc gttgaccggc acagtggaac ggatgtcgat tcgctccgtg ggcgtgcgcc
     5341 aggataccgg ggcgtatcac atcattccgt ggtcttcgat aaccaccttt gctaacttcg
     5401 tccgcggtat tggttcggta gtggcaaact acgatgttga tcgccatgaa gatgctgata
     5461 aagccaatca ggcactgaaa gatgcggtag cggaattaat ggaaaacgaa gaaattcgcg
     5521 ggctgattat tggtgaaccg aattttgccg ggattgtcgg cttaagcaat accgcgttta
     5581 cactgcgtgt ttcgttcacc acgctgccac tcaaacagtg gacggtacgc tttgccctcg
     5641 acagccaggt gaaaaaacat ttcgacctgg cgggcgttcg cgcgccagtg cagacttatc
     5701 aggtgctgcc tactccgggc gcgaccccgg ctgaaccgtt accgccgggg gagccaacgc
     5761 tttaacgctg gcgattgaca aaacggcggc gctgctcgtc gttcataaag gtccaggcaa
     5821 taaagcgact ctgcttttgc ccctgggcca tctctttttt taccaccttc accgcgccca
     5881 cgtcggtcag agcacgatac aacggcggta agttttcacc acgagatacc agcgaggtaa
     5941 accacatcac ctgcttcgcg aagcctttgc tctcttcaat catctttttg ataaaggcga
     6001 cttcaccacc ttcacaccac aactcctgtt gctgaccacc aaagttcagt gcatcgtctt
     6061 tgttcagccc caggttacgg cgtttacgct cactacctgc ccgcgctgca gcggcggaat
     6121 cgtggaatgg cgggttacac aaggtcgcgt cgtattgctc gtttttatgg atgatgccgt
     6181 taaaaattgc cccactctct ttttgccgac gcagacgaat ggcgcggtta agccccggat
     6241 tagcactgat aatcgcctgc gcactgctta acgcctggct gctggtttca ctaccggtaa
     6301 aacgccagcc atattcatgt acgccaatca gcggataaat acagttcgca ccaacgccga
     6361 tatccagaat gctggcattc gctggaattg ttccgcttgc ttcagccagt aaatcggcaa
     6421 ggtgatgaat ataatccgcc cggcccggta ctggtgggca gagaaaaccg tcggggatat
     6481 cccagttcgc tacggcgtaa aaatgggcca gcaacgcctt attgagcgcc ttcaccgcca
     6541 gcggattggc aaagtctacg ctttgctccc cggcgggtgt aagcgtgagg aattgcctga
     6601 gttcaggatt gacctgacaa agcgtggcga gatcgtagcg gctgtgatga cggttgcgcg
     6661 gatgcaaccc cggtttctgg gcggacatag cattctcctt taagcagcgg cgtaagatac
     6721 ccgcataacg cgacagggta aatatctggc tgcgtttatc attccccaaa accttaacat
     6781 ggctatggtt tatggcaatt catcataaaa atcagcatta tgcatgtcat taatttgtct
     6841 tattttgtaa aaatatatcg ttcaaaaaat aagcacatct ctgtcaattt tttgcgtcat
     6901 ttgcatcgcg caggcgacct acacttatca ggcactaccc gttgtattta tgaggatgtc
     6961 attatgaaaa agtgcctcac actactgatt gccaccgtcc tgagcggaat ctctctcacg
     7021 gcttatgccg cgcaaccgat gagtaacctg gacagcggtc agctacggcc cgccggtacc
     7081 gtttcggcaa ctggcgcatc aaacctaagc gatctggagg ataaactggc agaaaaagcg
     7141 cgcgaacaag gcgcgaaagg ttatgtcatt aattccgcag gcggaaatga ccagatgttc
     7201 ggtactgcaa ccatctacaa ataaccccac caacgtaact gcattcgcct ctggatgcag
     7261 ttttcgcctg ttttgtaatc aaaaaacatc aaatatgaac tcaatgtaaa taaatgtatt
     7321 tctttttcgc gcaatgggtg atagaaaatc gcgccaagtg ataatgctta tcaaaattat
     7381 tatcacttgc atgaacacta tcacgggatt aacagtggca tcgcatccgc agagatgctt
     7441 tctcgtggca gtgaaaattt caacatataa gaaaaagtca cctgcaaaat ggaaaacaat
     7501 cgcaatttcc ctgccagaca atttcattcg ctcacgttct ttgccggtct ttgtattggc
     7561 atcacgcctg tggctcaggc actcgccgcc gaagggcaaa ctaacgcgga tgacacgctg
     7621 gttgtcgaag catcaacgcc ttcgctttat gcgccacaac aatctgccga tccgaaattc
     7681 tcgcgtccgg tagcggatac tacccgcacg atgacggtga tttctgaaca agtgattaaa
     7741 gatcagggcg caaccaacct taccgacgcg ctcaaaaacg cccccggcg
//
LOCUS       DAZPAC010000194         7776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZPAC010000194 DAZPAC010000000
VERSION     DAZPAC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.194"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..1780)
                     /locus_tag="ACHUPM_003479"
     CDS             complement(<1..1780)
                     /locus_tag="ACHUPM_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFR0871162.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPEWLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHUPM_003480"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHUPM_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0871163.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHUPM_003481"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHUPM_003481"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR0871164.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHUPM_003482"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHUPM_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR0871165.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHUPM_003483"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHUPM_003483"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR0871166.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="ACHUPM_003484"
     CDS             complement(5968..7005)
                     /locus_tag="ACHUPM_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR0871167.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7338..>7776
                     /gene="yhhY"
                     /locus_tag="ACHUPM_003485"
     CDS             7338..>7776
                     /gene="yhhY"
                     /locus_tag="ACHUPM_003485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0871168.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1977 a         2101 c         1871 g         1827 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc attcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ttgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt atatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaag ccagcacccg
     7141 gctggctaag taatactgga agcaatgtga gcaatgtcgt gctttcaggt tctccgcgag
     7201 ggtcttcctg atcgcgagac aataataatc attctcattc gcacttgtcc aacacttttt
     7261 gcaaaaaaaa tgcatttgac tcgcatttga aagtcaatga tgttgaaagg gacatttacc
     7321 ccaaagagga caaaggaatg agtgagatag taatacgcca cgcagaaaca cgggattacg
     7381 aggccatcag gcagattcac gcccagccgg aggtgtattg caacacacta caggtgcctc
     7441 atccttccga tcatatgtgg caggagcgac tcgccgatcg tcccggcatc aagcaactcg
     7501 tcgcctgtat tgatggagac gtcgtgggcc atctcaccat tgacgtgcaa cagcacccac
     7561 gccgcagtca tgttgccgat tttggtatct gtgtcgactc tcgttggaag aaccgcggcg
     7621 tcgccagcgc cctgatgcga gagatgattg aaatgtgcga caactggttg cgggtagatc
     7681 gcattgaact aaccgtgttt gtcgataacg ccccggcaat taaggtctat aaaaaatacg
     7741 gctttgaaat tgaagggact ggtaagaagt acgcat
//
LOCUS       DAZPAC010000195         7730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZPAC010000195 DAZPAC010000000
VERSION     DAZPAC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.195"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            6..503
                     /locus_tag="ACHUPM_003486"
     CDS             6..503
                     /locus_tag="ACHUPM_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR0871169.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYILYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGYIKGACDQLCRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            500..970
                     /locus_tag="ACHUPM_003487"
     CDS             500..970
                     /locus_tag="ACHUPM_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0871170.1"
                     /translation="MSRVTAIISALVICIIVRLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(1059..1571)
                     /locus_tag="ACHUPM_003488"
     CDS             complement(1059..1571)
                     /locus_tag="ACHUPM_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR0871171.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSHSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1766..2530
                     /locus_tag="ACHUPM_003489"
     CDS             1766..2530
                     /locus_tag="ACHUPM_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871172.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            2481..3884
                     /locus_tag="ACHUPM_003490"
     CDS             2481..3884
                     /locus_tag="ACHUPM_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR0871173.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3884..5350
                     /locus_tag="ACHUPM_003491"
                     /pseudo
     CDS             3884..5350
                     /locus_tag="ACHUPM_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            5241..5984
                     /locus_tag="ACHUPM_003492"
     CDS             5241..5984
                     /locus_tag="ACHUPM_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR0871174.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5988..7208
                     /locus_tag="ACHUPM_003493"
     CDS             5988..7208
                     /locus_tag="ACHUPM_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR0871175.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            7212..7718
                     /locus_tag="ACHUPM_003494"
     CDS             7212..7718
                     /locus_tag="ACHUPM_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871176.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         2059 a         1842 c         2104 g         1725 t
ORIGIN      
        1 gaataatgtc gccatcatta cgcaaggctg ttgcagttgc tattggtggc ggggctgttg
       61 ctatagcatc tgtgttaatc actggcccag gtggtaacga tggtctggaa ggtgtcagct
      121 acatactata caaagatatc attggtgtat ggactgtatg tcacggacac accggaaaag
      181 acatcatgcc tggtaaaacg tataccgaag cagaatgcaa agctctcctg aataaagacc
      241 ttgccactgt cgccagacaa attaacccgt acatcaaagt cgatataccg gaaacaacgc
      301 gcggcgctct ttactcgttc gtttacaacg tgggcgctgg caatttcaga acatcgacgc
      361 ttcttcgcaa aataaaccag ggctatatca aaggcgcatg tgaccagcta tgtcgctgga
      421 catatgctgg cggtaagcaa tggaaagggc tgatgacccg tcgtgagatt gagcgtgaag
      481 tctgtttgtg ggggcagcaa tgagcagagt caccgcgatt atctccgctc tggttatctg
      541 catcatcgtc cgcctgtcat gggctgttaa tcattaccgt gataacgcca ttacctacaa
      601 agagcagcgc gacaaaaacg caagagaact gaagctggcg aacgcaacca ttactgacat
      661 gcagcagcgc cagcgtgatg ctgatgcact cgatgctaaa tacacgaagg agttagctga
      721 tgcgaaagct gaaaatgatg ctcttcggcg caagcttgat aatggtggtc gggtgctcgt
      781 caaaggaaaa tgccctgtgc catcctcagc cgaaacctcc agcgcctccg gcatgggcaa
      841 tgatgccacc gtcgaactct ctccagttgc tggacgaaac gttctcggta tccgggacgg
      901 aatcatcagc gaccagacag cattgagaat gcttcaggag tacatcagga tccaatgcct
      961 tggggggtag cggtaatttt actcattatc cttcaaatca gattctgttg tcagaggaat
     1021 gggggaagct ggattcgcaa gagcttttta tgggcagttt agctaaaaaa aggcaacacg
     1081 aagctaaaac taccaacata aagtgctgac cagctagcag ctgaaatcag actcactgta
     1141 tacactttca ctggatgaag ttgcaacatt cctgccacca aaggagcaat ataacgcagc
     1201 acggcgataa agcgcgaagt gaaaagtatt gacacagagt tattttgcaa ctgaaggcga
     1261 acccgttcta ttgtattgga atggcttgat attatacatg caagttgagg gatatgccca
     1321 attattatac caagatggta attaacgata gtaccgcacc atgcgccact catgaccgtg
     1381 attccagctt cccatggtga aagagtcgtc ttgctgactg taattacagt ggccatcata
     1441 attgatgctg gtggcaatat cgccgaaatg agtaaagtgg attttgtgaa cgctattata
     1501 aaaagaagcc cccataggcg atatggatga agtacaaaat agttcatcaa agcatttatc
     1561 cactccatca atgtctttcc ttctgtgaat ttttctttgc tatttaaccg tgatacgact
     1621 taatttaggc ttaaacacct tacatttaca aggtactcct gtggggggcc ctgccacgga
     1681 gcgtcgcagg agtgagtgat gaggaagaat taaagcaacc tattgtgaca ggcagcgata
     1741 acagtaaatc acaggaagca taatcatggc aaaaccggac tgggaggcca tcgaatcggc
     1801 ataccgggcc ggagtcctta gcctccgtga tataggcgag aaatacggcg ttacagaagg
     1861 ggctatcagg aagagggcca aaaagcttgg ttgggcacgc agtggcggta cgcaggtttg
     1921 caaaaatggt acgcaaaaaa ggaaagtgcg taccagcaga aagcctgcca ttactggcct
     1981 tacacaaaaa agtacgcaac taaaaacaga atctacaccg gatacgaaac cgatacgcgg
     2041 aatgcgtacc gatcccccga ctaacccatt ccaacccggt aaccagcagg cattaaaaca
     2101 cggtggttat gcccgtcgcc ttctgctcaa agatgaggtg atagaggacg ctaaagcgtt
     2161 gacgctcgag gacgaattat ttcgccttcg tgctaacaac cttgtcgctg cagagaatat
     2221 tggtcggtgg ttggtgtcgc tggaagatgc taatggggac caggaaagga agatgctgat
     2281 tgaaaatatc agcgccgccg agaaagcaat gatgcgcaat acagttcgta ttgagtccat
     2341 cgttggcacg cttgcgacgg taggaaaaat atttgctgat acagcctacc gcaaggccgc
     2401 cactgataag gtgtctctgg aggctgatcg tcttcgccgt gatgcaggta ttgatgatgg
     2461 caatggagag cgtgacctca atgacttcta ctctgacatc caaaccgacg ctgaatccgg
     2521 ctttacgtag tttctggact atgcgggcac gtaacaaagt gctttatggt ggtcggtcat
     2581 cgtcaaaatc atgggatgcc gctggcattg ccatatttct gtcgaataaa tacaccctgc
     2641 gtttttgttg tgcccgtcag atccagaata aaatcgaaga gtcggtgtat accctgctca
     2701 aaattcagat agacaggttt ggtctgcggc accgtttccg tattctgaac aacaaaatca
     2761 ttaaccgggt tactggctcg gaatttgttt tttatggatt atggcgcaac atcgaagaaa
     2821 ttaagtcact ggaggggatc gatgtgttgt ggctggaaga agcccacgca ctgacggaat
     2881 accagtggaa aattctggag ccaacgatcc gtaaagaggg ttcggaatgc tggttcatat
     2941 tcaaccccgg acttgttact gatttcgtct ggcgcaactt cgttgttgat ccgcccgaag
     3001 gcactctcat ccgcaaaatt aactatgacg aaaatccgtt tctgtctgac accatgctta
     3061 aggttatcga cgcggcgcga cgccgtgatc cggatggttt taaacatgtg tatgagggcg
     3121 ttccggagtc tgatgatgat gcggcaatca tcaaactgtc ttggatagaa gccgcagtgg
     3181 atgcgcacaa aacgttaaat ttcgaaccca gtggaagaaa gcgtattggc tttgacgtgg
     3241 ctgacagtgg tacagataag tgcgctaacg tttaccgtca cggatccgtt gttttctggg
     3301 ccgacgaatg gaaggccaaa gaagatgaat tactgaagag ctgccagcgt acttatcagg
     3361 cggcgctgga gcgtgaagca gatattgttt acgactctat cggtgttggt gcgtctgccg
     3421 gtgctaaatt ctctgaaatt aacgctgacc ggaagagcga gaacgcatac gcgcgacgtg
     3481 tgaattacca gaggtttaac gccggtgctg gtgtgcatga gccagatgac gagtacaacg
     3541 gcatccccaa caaagacttt ttcgcaaatc ttaaggctca ggcatggtgg ctggtggctg
     3601 accgtttcag aaatacgttt aacgccatta acaacggaga acagtatcct gtggatgagc
     3661 tgatcagcat agattctcgt tgtccgttgc ttgaaaagct gaaactggaa ctgacaacac
     3721 ctcatcgtga tttcgaccgt aacggacgtg tgatggtcga aagtaaaaaa gacctcgcaa
     3781 aacgcgagat accgtcacca aacgttgctg atgcattcat tatggccttc gcgccaatcg
     3841 atacatcgct ggatatctgg gaacagctgg ggagacaggc ctgatggcac gaaacaaaca
     3901 agccctgcgg cgaactgtgc aggccacagc tgatggttat gagaatttta ttgcccgcgt
     3961 agggatgcag acacctaacc agcactcagc atccacctac cgggctaatt tcaccagtcg
     4021 taaccgcatg ctggtggaat ggtcctatcg ttcatcctag atcatcggcg aagcagtcga
     4081 tgctatcccg gatgatatga cccgcaaagg cattcgcatc acttcggaaa ttgatgcaaa
     4141 agatcgtggc attctcgaat cacaactgga tgagttgcaa atctgggatg cgctgaatga
     4201 cgtgctgaaa tggtcgcgcc tctacggcgg cgcggtgggt ttcatcatga ttgaggggta
     4261 ggcaccaatg accccgctgc gacccgaaac catcggtaag ggcaagttta aggggattct
     4321 cccgctcgac cgctggatgg tcgacccggc actgacccgc cgcattaaag atatggggcc
     4381 ggacctgggt aaacctgagt tttacgatgt ggtgaccaca gcaacgggaa ttcctgcctg
     4441 gcgcattcat cacagtcgcc tgattcgctt tgatggcgtc acgttgccat ttcagcagaa
     4501 gatgaccgag aacgaatggg gaatgtcggt tgtagagcgt atctgggatc gtcttaccgc
     4561 gttcgacagc gctactgtcg gcgcggcgca gctggtctac aaggcgcatt tgcgtaccta
     4621 cagcgtggag aagctacgcg agcttatcgc acttggtggt cctgcgtatg aagcgttgct
     4681 gaagaatatc gacctgattc gacagttcca gagtaatgaa ggcatgacgc tcatggactc
     4741 gcgggataag tttgaaaccc atcagtacag cttcagtggt ctggatgaca tcctttcgca
     4801 gtttgtagaa cagattagtg gcgctgttgg tatcccactg gtgcggttgt tcggacagtc
     4861 cccgaaagga ttttctaccg gtgatgcaga ccttgccaac tattacgatc gcatcagttc
     4921 gttgcaggag aggcgtttac gtcttccggt gcggcggata ctggacatca tgcatcgttc
     4981 ggaacttggc aagccgctgc cggacgattt cacgtttgag tttaacccgc tctggcaaat
     5041 gtctgatgtc gatcgctcaa cggtggcgtt aaacaccacc aacgcaatca gtacggcgct
     5101 gggtgatggt ctgatgacac tgaaagccgc tatgactgat ttgcgagaaa attctgacgt
     5161 aaccggcatc ggggcatcca ttaccgacga ggacatcgag aatgccgaag atgaagcgcc
     5221 gcccggcatc ggcgaacctg atgacgaacc gcaggagccg tcaggcggaa atccggtatc
     5281 gaaccagcct acgcaggata gcgagggcgg tcggagacat cgtaaatggt cgctacgatg
     5341 gttcaaatga cagtatcacg gaaattattg cggcgctgga gcgttacagt gaaatcatca
     5401 ccccctgggc gacaaaggtc gcggaaaact ttactgcgga cctaacccgg cagaacgaga
     5461 aagtttggcg gcaacacagc aagaacatca gtcgcgagct ccgcaatctt gtggaaagcg
     5521 ctcctgtggg ccaggtgatg caatccatca tcgccgaaca ggtcaagtac atcaaatctc
     5581 tgcctctcga ggccgcagac agggtgtacg acatccagaa caaagcgata gaggctgttg
     5641 tcaccggtgg tcgggcggag cagtttgcta aagagattgc atctaccggc gatgttgcta
     5701 agtctagggc cgatctgatt gcccgaacgg aactgggaag agcaacgggc gcgctggata
     5761 tggcccgagc gatagctatt ggctcggatg gttatatttg gcgtacagcc gatgatggcg
     5821 atgtccgaga ttcccacgat cacatgaaag gtaaattcgt ccgctgggat tcacctccaa
     5881 ctttggacgg catgaccggc cacgcgggcg agctgccaaa ctgccgctgc tataaagaga
     5941 tcgtgtttgt tcgcgttcca ttcgcaatga aaagggcagc ataacccatg aaatactttt
     6001 ttgagaccag gctcggggaa acccgatacc gcctggctga cggctcgttg ctgtgcaaag
     6061 acgtgccgat aggacgaaca ggtaagcagc tctatggtgc tgatgacctg ccaaaactga
     6121 aacccgataa gttcggtgaa atagtcgtca cgcgttctcc tgagcaggta ttccatccgg
     6181 ccacgcttgc ctcattcgaa gggatgagca tcacgatcct gcatcctgaa gatgaaaacg
     6241 ggaatgtgcg gctggtaaat cccgagaact ggaaagagct tgctgtcggg cacctccaga
     6301 atgtccggcg cgggacgggt gagcagtctg atttgatgct ggctgacctt atcgtcaaag
     6361 acgaaagcgc cattcagctt atcgaagatg gcctgcgcga agtgtcgtgc ggctatgacg
     6421 cggagtacga gcagaccgag ccaggtaaag ccgagcaggt cgatattacc ggaaaccatg
     6481 tggctcttgt ccctaaaggc agagccggaa atcgttgtgc aattggagac agagacacaa
     6541 tggcaaatca aaagaaaaac tggtggaacc gcatgcgtgc ggccatcaag acaggagatg
     6601 ccgacaccat gaacgaactg gtggagtcgg ctcccgcatc ggttacagga gatgaggggg
     6661 atttgccgca gggcgttaat ctcaacatca acctgtcccc gcagcaacca ctaccggaca
     6721 aagcaccaga gatgggcgga gatccaaccg gcgacagtga tgatgacctc aaaacattac
     6781 tgaaagccct gctggctaag ctggaaagaa atgccacggg cgataacgat aataagcctg
     6841 acgataatcc gaccggtgac ggcgaggacg atgaagagga aaccacgatt actggtgact
     6901 cagcctggcg tgccgaagtt atcgttccgg gtatcgatct gagccgtaag atgaaaccga
     6961 ccgcgttcaa acgcgaggtt ctggcttctg ctgacaaaac gctggttcgc cagatagtcg
     7021 gtgatgcgga tatccgcaaa ttaccgaaac aatcggtcga catggcgttt aatgccgtgt
     7081 ctgaggttgc caaagggcga aacacccgcg ccaccaccgg cgatgcacag cgcctaaaca
     7141 tgggcatgac cagtatcgct tccctgaaca aacaaaacgc tgaattctgg gcaaaccgta
     7201 aagggtaaaa aatgaataat gtatttctgt accggatgcc tgttggcatt gccggggctg
     7261 tctctcgccc gcaggactta accgtcgaac cggtggtcct taaatccgat aacgccttcg
     7321 ctgcctatgg cctggctggt aaatacgatg atgacggttt tttcgtgccg ctggcagatg
     7381 gtgataccgc agacaaggtg aaggggatct acgtgcgccc ttatccgacc acgtcgcagc
     7441 cggacatggt tcgccaggtg ggaacaggca agaacttccc gggcgacgcc atgaagcgtg
     7501 gctacgtgac cgttaatctc ggttctgatt ttgatgccag cactatcaaa aaaggcgatc
     7561 cggtatacgt tgtcgtctcc actgatgaat ccatcaaagt gccgctgggt ggattcatgt
     7621 ccacgtcagt cagtggcaaa aacgtggtgc tgaccaacgc tgaattcaca ggtgccggtg
     7681 atgctgacgg caatgcagaa atttcctgga agatttaagg aacagacgaa
//
LOCUS       DAZPAC010000196         7717 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZPAC010000196 DAZPAC010000000
VERSION     DAZPAC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.196"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..643
                     /locus_tag="ACHUPM_003495"
     CDS             <1..643
                     /locus_tag="ACHUPM_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0871177.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="ACHUPM_003496"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="ACHUPM_003496"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFR0871178.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="ACHUPM_003497"
     CDS             2474..2986
                     /locus_tag="ACHUPM_003497"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR0871179.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="ACHUPM_003498"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="ACHUPM_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR0871180.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="ACHUPM_003499"
     CDS             5579..5782
                     /locus_tag="ACHUPM_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0871181.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHUPM_003500"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHUPM_003500"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR0871182.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7717)
                     /locus_tag="ACHUPM_003501"
     CDS             complement(6827..>7717)
                     /locus_tag="ACHUPM_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR0871183.1"
                     /translation="DRVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQA
                     ATSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAV
                     PMPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQE
                     VVLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNR
                     GTENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLW
                     LTKQDVEAHKESQEVLHVQR"
BASE COUNT         1785 a         2102 c         2020 g         1810 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt ttgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaaaaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccacactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg gacgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcgatc
//
LOCUS       DAZPAC010000197         7623 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZPAC010000197 DAZPAC010000000
VERSION     DAZPAC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.198"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(152..487)
                     /locus_tag="ACHUPM_003502"
     CDS             complement(152..487)
                     /locus_tag="ACHUPM_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR0871184.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="ACHUPM_003503"
     CDS             complement(491..835)
                     /locus_tag="ACHUPM_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR0871185.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHUPM_003504"
     CDS             complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHUPM_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR0871186.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHUPM_003505"
     CDS             1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHUPM_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR0871187.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="ACHUPM_003506"
     CDS             1257..1376
                     /locus_tag="ACHUPM_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR0871188.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHUPM_003507"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHUPM_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR0871189.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHUPM_003508"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHUPM_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR0871190.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHUPM_003509"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHUPM_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR0871191.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="ACHUPM_003510"
     CDS             4176..4544
                     /locus_tag="ACHUPM_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR0871192.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHUPM_003511"
     CDS             complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHUPM_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR0871193.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="ACHUPM_003512"
     CDS             5295..5588
                     /locus_tag="ACHUPM_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR0871194.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="ACHUPM_003513"
     CDS             5660..6319
                     /locus_tag="ACHUPM_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR0871195.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="ACHUPM_003514"
     CDS             6411..6857
                     /locus_tag="ACHUPM_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR0871196.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..>7623)
                     /gene="hlyE"
                     /locus_tag="ACHUPM_003515"
     CDS             complement(7064..>7623)
                     /gene="hlyE"
                     /locus_tag="ACHUPM_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR0871197.1"
                     /translation="VLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEK
                     SSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQS
                     FFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAA
                     KKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1957 a         1714 c         1772 g         2180 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc
     2281 gcgtaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ctccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 cct
//
LOCUS       DAZPAC010000198         7606 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZPAC010000198 DAZPAC010000000
VERSION     DAZPAC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.199"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            95..1384
                     /gene="hpaG"
                     /locus_tag="ACHUPM_003516"
     CDS             95..1384
                     /gene="hpaG"
                     /locus_tag="ACHUPM_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR0871198.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            1381..2847
                     /gene="hpaE"
                     /locus_tag="ACHUPM_003517"
     CDS             1381..2847
                     /gene="hpaE"
                     /locus_tag="ACHUPM_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0871199.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2849..3700
                     /gene="hpaD"
                     /locus_tag="ACHUPM_003518"
     CDS             2849..3700
                     /gene="hpaD"
                     /locus_tag="ACHUPM_003518"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR0871200.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3710..4090
                     /locus_tag="ACHUPM_003519"
     CDS             3710..4090
                     /locus_tag="ACHUPM_003519"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR0871201.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4201..5004
                     /gene="hpaH"
                     /locus_tag="ACHUPM_003520"
     CDS             4201..5004
                     /gene="hpaH"
                     /locus_tag="ACHUPM_003520"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR0871202.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            5015..5803
                     /gene="hpaI"
                     /locus_tag="ACHUPM_003521"
     CDS             5015..5803
                     /gene="hpaI"
                     /locus_tag="ACHUPM_003521"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR0871203.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5978..7354
                     /gene="hpaX"
                     /locus_tag="ACHUPM_003522"
     CDS             5978..7354
                     /gene="hpaX"
                     /locus_tag="ACHUPM_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR0871204.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            7364..>7606
                     /locus_tag="ACHUPM_003523"
     CDS             7364..>7606
                     /locus_tag="ACHUPM_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR0871205.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         1748 a         2133 c         2152 g         1573 t
ORIGIN      
        1 tttacatcac ttacttttgc caaactacgc aatcgggaat aaaagcaatc attaacatat
       61 taatgattaa gatgatcgaa tccgaggagt ggtaatgaaa ggcactatct tcgccgtagc
      121 gttgaaccat cgcagccaac ttgatgcatg gcaggaagcg ttccagcaat ccccctacaa
      181 agccccgcct aaaactgcgg tctggtttat taaaccgcgc aatacggtga ttggttgcgg
      241 tgaaccgatt ccctttccac agggtgaaaa ggtactgagc ggtgcgactg ttgcgctgat
      301 tgtgggaaaa acggcgacga aagtacgtga agaagatgcg gcagagtaca tcgccggata
      361 tgcgctggct aacgacgtca gcctgccgga agagagcttt taccgcccgg caatcaaagc
      421 aaaatgtcgt gatggattct gccccattgg cgaaaccgtg gctctcagca atgtcgataa
      481 tctgaccatc tataccgaga tcaacgggcg tcctgccgat cactggaata ccgccgattt
      541 acaacgtaac gccgcgcagt tgctgagcgc cctgagcgaa tttgccacgc tgaatccagg
      601 cgacgccatt ctgctcggca cgccacaggc gcgcgtggaa atacagccag gcgatcgcgt
      661 tcgtgttctc gcagaaggtt tcccgccgct ggaaaatccg gtagtggacg aacgtgaagt
      721 gaccacgcac aagagcttcc caacgcagcc acacccgcac ggcacgctgt ttgccctcgg
      781 cctgaactac gccgaccacg ccagcgaact ggaatttaag ccaccggaag aaccgctggt
      841 gttcctgaaa gcgccaaata ccctcactgg cgataaccag acctccgtgc gtccaaacaa
      901 tattgaatac atgcactatg aagcggagct ggtggtagtt attggcaaac aggcgcgtaa
      961 cgtcagcgaa gccgatgcca tggattatgt cgcgggctac accgtgtgta acgactacgc
     1021 cattcgtgac tatctggaaa actactaccg ccctaacctg cgggtaaaaa gccgcgacgg
     1081 actgacgccg atgctttcaa ccatcgtgcc gaaagaggcg atcccggacc cgcataatct
     1141 gacccttcgc accttcgtca acggcgagtt acgccagcaa ggcaccaccg ccgatctgat
     1201 cttcagcgtg cccttcctga tcgcctactt aagtgaattt atgaccctga atccgggcga
     1261 catgatcgcc accggcacac ccaaaggctt atctgacgtg gtgcctggcg atgaagtagt
     1321 ggtggaagta gaaggcgtgg gccgcctggt gaaccgaatt gtgagtgagg atacagcgaa
     1381 atgaaaaaag taaatcattg gatcaacggc aaaaatgttg caggtaacga ctacttccag
     1441 accaccaatc cggcaacggg tgaagtgctg gcggatgtgg cctctggcgg tgaagcggag
     1501 atcaatcagg cggtagcggc agcgaaagag gcgttcccga aatgggccaa tctgccgatg
     1561 aaagagcgtg cgcgcctgat gcgccgtctg ggcgatctga tcgaccagaa cgtgccagag
     1621 atcgccgcga tggaaaccgc ggacaccggc ctgccgatcc atcagaccaa aaatgtgttg
     1681 atcccacgcg cttcccacaa ctttgaattt ttcgcggaag tctgccagca gatgaacggc
     1741 aagacctatc cggttgacga caagatgctc aactacacgc tggtgcagcc ggtgggcgtt
     1801 tgtgcgctgg tatcgccgtg gaacgtaccg tttatgaccg ccacatggaa ggtcgcgccg
     1861 tgtctggcgc tgggcaatac cgcggtactg aaaatgtcgg aactctcccc gctgaccgct
     1921 gacagcctgg gtgagctggc gctggaagcc gatattccgg caggcgtgct gaacgtggta
     1981 cagggctacg gcgcaaccgc aggggatgcg ctggttcgtc atcatgacgt acgtgccgtg
     2041 tcgttcaccg gcggtacggc caccgggcgc aacatcatga aaaacgccgg gctgaaaaaa
     2101 tactccatgg aactgggcgg taaatcgccg gtgctgattt ttgaagatgc cgatattgaa
     2161 cgcgcgctgg acgccgccct gttcaccatc ttctcgatca acggcgaacg ctgcaccgcc
     2221 ggttcgcgca tctttattca gcaaagcatc tacccggaat tcgttaaacg ctttgccgaa
     2281 cgcgccaacc gtctgcgcgt gggcgatccg aacgatccga atacccaggt tggcgcgctt
     2341 attagccagc agcactggga aaaagtctcc ggctatatcc gtctcggcat tgaagaaggc
     2401 gcaaccctgc tggcgggcgg cccggataaa ccgtccgacc tgcctgcgca cctgaaaggc
     2461 ggcaacttcc tgcgcccaac cgtgctggca gacgttgata accgtatgcg agtcgcccag
     2521 gaagagattt tcgggccggt cgcctgcctg ctgccgttta aagacgaagc cgaaggctta
     2581 cgtctggcaa acgacgtgga gtacggcctc gcgtcgtaca tctggacaca ggatgtcagc
     2641 aaagtgttac gcctggcgcg tggcattgaa gctggcatgg tgttcgtcaa cacccagaac
     2701 gtgcgtgacc tgcgccagcc atttggcggc gtaaaagcct ccggcaccgg gcgtgaaggc
     2761 ggtgagtaca gcttcgaagt gttcgcggaa atgaagaacg tctgcatttc catgggcgac
     2821 catccaattc cgaaatgggg agtctgatat gggtaagtta gcgttagccg ccaagatcac
     2881 gcacgtaccg tcgatgtatc actctgaact gccagggaaa aaccacggtt gccgccaggg
     2941 cgcgatcgac gggcataaag agatcagcaa gcgttgccgg gaaatgggcg tcgataccat
     3001 tatcgttttc gatacccact ggctggtcaa cagtgcttat cacatcaact gtgcagacca
     3061 ttttgaaggc gtctacacca gcaacgagct gccgcatttt atccgcgata tgacctacaa
     3121 ctacgagggc aacccggagt tggggcagct tattgccgat gaagccttaa agctcggcgt
     3181 gcgggcaaaa gcgcacaaca ttcccagcct gaaactggaa tacggcacgc tggtgccgat
     3241 gcgctacatg aatgaagaca agcacttcaa agtggtctcc atttcggctt tctgcacggt
     3301 tcacgatttt gccgacagcc gcaagctggg cgaagcgatt ctgaaagcga tcgaacagta
     3361 cgacggcacc gtggcggtcc ttgccagcgg ttcgttatcg caccgcttta ttgacgatca
     3421 gcgtgcggaa gaagggatga acagctacac ccgcgagttc gaccgccaga tggacgagcg
     3481 cgtggtgaag ttgtggcgcg aaggccagtt caaagagttc tgcaatatgc tgccggagta
     3541 cgccgactac tgctacggcg aaggcaatat gcacgacacg gtgatgctgc tggggatgct
     3601 cggctgggat aaatacgacg gcaaggtgga gtttatcacc gagctattcc caagctctgg
     3661 caccggtcag gttaacgctg ttttcccgct tcccgcgtaa ggacgtttta tgccgcactt
     3721 tatcgttaaa tgcagtgaca acatccgcga agaagccgac ctgccggggt tgttcgccaa
     3781 agtgaatccg acgctggcag ccacgggtat ttttccgctg gcgggtattc gcagccgcgt
     3841 gcattgggtc gatacctggc agatggccga cgggcagcgt gattatgcct tcgtgcatat
     3901 gacgttgaaa atcggcgcag gtcgcagcct ggaaagccgc cagcaggcgg gtgaaatgct
     3961 gtttgaactg attaaaacgc acttcgccgc cctgatggag agccgcctgc tggcgttgtc
     4021 gtttgagatt gaagagctgc atccgacgct gaattttaaa caaaacaacg tgcacgcatt
     4081 gtttaagtag cgcgcagatt gcccggtggc gctgcgctta ccgggcctac aaaaccccaa
     4141 accgtacacc gtaggccgga taaggcgcag ccgcatccgg caatgccaca ggatatcgct
     4201 atgttcgata aacacaccca caccctgatc gcccagcgtc tggatcaggc agaaaaacag
     4261 cgcgaacaga tccgcgcgat ctcgctggat tacccggaga tcaccatcga agacgcttac
     4321 gcggtgcagc gtgaatgggt tcgcctgaaa atcgccgaag gtcgcacgct gaaaggccac
     4381 aaaatcggcc tgacctcgaa agcgatgcag gcaagctcgc agatcagcga accggattac
     4441 ggcgcgctgc tggatgacat gttcttccac gatggcagcg atatcccgac cgatcgcttt
     4501 atcgtgccgc gcattgaagt ggagctggct ttcgtgctgg caaaaccgct gcgtgggcca
     4561 aactgcacgc tgttcgacgt ctacaacgcc acggactacg tgatcccggc gctggagctg
     4621 atcgacgctc gctgccacaa catcgatccg gaaacccagc gcccgcgcaa agtgttcgac
     4681 accatttctg ataacgccgc caacgccggg gtgatcctcg gcggtcgtcc gatcaaacca
     4741 gacgaactgg atctgcgttg gatctccgcc ctgatgtatc gcaatggcgt gattgaagaa
     4801 accggcgtcg ccgctggcgt gctgaatcat ccggcaaacg gcgtggcctg gctggcgaac
     4861 aaactcgcac cgtatgacgt acaactggaa gccgggcaaa tcattctcgg tggttcgttc
     4921 acccgcccgg ttccggcgcg taagggcgac accttccacg tcgattacgg caacatgggt
     4981 tccattagct gccgctttgt ttaaggagag aacgatggaa aacagcttta aagcggcgct
     5041 gaaagcaggc cgtccgcaga tcggattatg gctggggctg agtagcagct acagcgcaga
     5101 gttactggcc ggagcaggat tcgactggtt gttgatcgac ggtgagcaca cgccgaataa
     5161 cgtgcaaacc gtgctcaccc agctacaggc gattgcgccc taccccagcc agccggtagt
     5221 acgtccgtcg tggaacgatc cggtgcaaat caaacaactg ctggacgtcg gcacacaaac
     5281 cttgctggtg ccgatggtac aaaacgctga cgaagcccgt gaagcggtac gcgccacccg
     5341 ttatcccccc gccggtattc gcggtgtggg cagtgcgctg gcccgcgcct cgcgctggaa
     5401 tcgtattcct gattacctgc aaaaagccaa cgatcaaatg tgcgtgctgg tgcagatcga
     5461 aacgcgtgag gcaatgaaga acttaccgca gattctggac gtggaaggcg tcgacggcgt
     5521 gtttatcggc ccggcggatc tgagcgccga tatgggttat gccggtaatc cgcagcaccc
     5581 ggaagtacag gccgccattg agcaggcgat cgtgcagatc cgcgaagcgg gcaaagcgcc
     5641 ggggatcctg atcgccaatg agcaactggc aaaacgctat ctggaactgg gcgcgctgtt
     5701 tgtcgccgtc ggcgttgaca ccaccctgct cgcccgcgcc gccgaagcgc tggcagcacg
     5761 gtttggtgcg caggccaccg ccgtgaagcc cggcgtgtat taatgccgga tgcgctgcgc
     5821 ttatccggcc tacactcgca ccaaaccgta ggccggataa ggcgtttacg ccgcatccgg
     5881 caaaaagccg taccaaatcg cggaccggat aaggcgttac gccgcatccg ccataaacct
     5941 tgtcgtaccc tacaaaaatc ccattagagg aagaaaaatg agcgacacct cacctgccat
     6001 accggagagt atcgatccgg cgaatcagca taaagcgctg actgccggac aacaggcggt
     6061 tattaagaag ctatttcgcc gcctgagcgt ctttctgttc gtgctgttta tcttctcgtt
     6121 ccttgatcgc atcaacatcg gctttgccgg actcacgatg ggacgcgacc tcggtctgag
     6181 cgccaccatg tttggcctcg ctaccaccct gttctatgcc gcttatgtca tcttcggcat
     6241 tcccagcaac attatgctga gtattgtcgg tgcgcggcgc tggatcgcca ccatcatggt
     6301 gctctggggc atcgcctcta ctgccaccat gtttgccact ggccccacca gcttgtacgt
     6361 actgcgtata ctggttggca ttaccgaagc cggctttctg cctggtattc tgctgtattt
     6421 aaccttctgg tttccggcct atttccgcgc ccgtgccaac gccttgttta tggtagcaat
     6481 gccggtaacg acagcgttgg gctcgatcgt ttccggctac attttgtcgc tggatggcgt
     6541 catggcatta aaaggctggc agtggctgtt tttgctggaa ggcttcccgt cggtattact
     6601 cggcgtcatg gtgtggttct ggcttgatga ctcaccggac aaagctaagt ggctgacgaa
     6661 agaagacaaa aaatgcctgc aagagatgat ggataacgat cgtctgacgc tggttcagcc
     6721 agagggagcc atcagccacc acgccatgca acaacgcagt atgtggcggg agatcttcac
     6781 tccggtggtg atgatgtata ccctggcgta tttctgcctg accaacacac ttagtgcgat
     6841 cagcatctgg acaccgcaga tcctgcaaag ttttaatcag ggcagcagta atatcaccat
     6901 cggcatgctg gccgccgtac cgcagatttg taccattctc gggatggtct actggagccg
     6961 tcactcagat cgccgccagg aacgaaggca tcacaccgcc cttccttatt tgttcgctgc
     7021 cgcaggttgg ttactggctt cggcaactga tcacaacatg atccagatgc tggggatcat
     7081 tatggcttcg accggatcat tcagcgcaat ggcgattttc tggacaacac cggatcagtc
     7141 catcagcctg cgggcacgag cgatcggtat tgcggtgatc aacgccactg gcaacattgg
     7201 ttcagcgtta agtccgttta tgatcggctg gttgaaagat ttgaccggca gctttaacag
     7261 tggattgtgg tttgttgccg cgctgctggt gattggtgcg gggattatct gggcgattcc
     7321 aatgcagtcc tcccgtccgc gagcgaccct gtaaggaacg acgatgtgtg accgtcagat
     7381 tgccaatatt gatatcagca aagagtacga tgaaagcctg ggcacggacg atgtgcatta
     7441 tcagtcgttc gcccgcatgg cggcattttt tggccgccat atgctgccac atcgccacga
     7501 acagtacttt cagatgcatt tcctcaatag cggacagatt gagctacagc ttgacgatca
     7561 tcgctactcg gtggaagcgc ccctgtttgt cctgacgccg ccgtca
//
LOCUS       DAZPAC010000199         7563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZPAC010000199 DAZPAC010000000
VERSION     DAZPAC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.200"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..691
                     /locus_tag="ACHUPM_003524"
     CDS             <1..691
                     /locus_tag="ACHUPM_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871206.1"
                     /translation="EISLPLLTKMSSERTLAVQAALMQQPDKSLALLAWTLCLNVFDS
                     GAYSKPAQISLECKHYSLTSDAPSGKEGAAFLALMAEKARLAALLPEGWSRDMTTFLS
                     LSQEVLLSLLSFCTACSLNGVQTRECGRTSRSPLDSLDSAIGFHMRDWWQPTKANFFG
                     HLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKDNRWVPGWMCSPRPQTDA
                     TERADNLADAA"
     gene            744..1178
                     /gene="psiB"
                     /locus_tag="ACHUPM_003525"
     CDS             744..1178
                     /gene="psiB"
                     /locus_tag="ACHUPM_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR0871207.1"
                     /translation="MKTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMVEGGQA"
     gene            1175..1894
                     /locus_tag="ACHUPM_003526"
     CDS             1175..1894
                     /locus_tag="ACHUPM_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFR0871208.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            1891..2487
                     /locus_tag="ACHUPM_003527"
     CDS             1891..2487
                     /locus_tag="ACHUPM_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871209.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            2951..3376
                     /locus_tag="ACHUPM_003528"
     CDS             2951..3376
                     /locus_tag="ACHUPM_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR0871210.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            3423..3845
                     /locus_tag="ACHUPM_003529"
     CDS             3423..3845
                     /locus_tag="ACHUPM_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR0871211.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            3842..4033
                     /locus_tag="ACHUPM_003530"
     CDS             3842..4033
                     /locus_tag="ACHUPM_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871212.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(4306..4554)
                     /locus_tag="ACHUPM_003531"
     CDS             complement(4306..4554)
                     /locus_tag="ACHUPM_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871213.1"
                     /translation="MSRGPPQRRRARARNGVAPYIPAAFCRDAGSAGHGATEAARPER
                     SLQARGFHASDRSGSPKPRQRVRRRLAGAPAAACRSRA"
     gene            4728..4847
                     /locus_tag="ACHUPM_003532"
                     /pseudo
     CDS             4728..4847
                     /locus_tag="ACHUPM_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332787.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            5071..5301
                     /locus_tag="ACHUPM_003533"
     CDS             5071..5301
                     /locus_tag="ACHUPM_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871214.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPTPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            5353..6714
                     /locus_tag="ACHUPM_003534"
     CDS             5353..6714
                     /locus_tag="ACHUPM_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFR0871215.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            6761..7324
                     /locus_tag="ACHUPM_003535"
     CDS             6761..7324
                     /locus_tag="ACHUPM_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR0871216.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREVLPCGTFAYTRVPTMIIRLRA"
BASE COUNT         1637 a         2062 c         2260 g         1604 t
ORIGIN      
        1 ggaaatcagt ctgccgttgc tgacgaaaat gtcttccgaa cgcacgctgg cagtccaggc
       61 agcactgatg cagcaaccgg acaaatctct tgcactgttg gcatggacgc tctgcctgaa
      121 tgtgtttgac agcggagcgt acagtaaacc agcacaaatc agcctggaat gtaaacatta
      181 ttcgctgacc agcgatgcgc cgtcagggaa ggaaggtgcc gcattcctgg cgctgatggc
      241 agaaaaagcc cgtcttgcgg ccctgttacc ggagggatgg tcacgggaca tgacgacgtt
      301 cctgtcactc agtcaggagg tgctgttatc cctgctcagt ttctgcaccg catgcagcct
      361 taacggtgtc cagacccgcg agtgtggtcg cacgtcacgc agtccgcttg actcactgga
      421 tagcgccatc ggttttcaca tgcgcgactg gtggcagccg acgaaagcaa acttcttcgg
      481 acacctgaaa aagccgcaga ttatcgcagc cctgaatgat gccggactgt ccggtgccgc
      541 acgggacgca gagaagatga agaaaggcga tgccgctgaa catgcagagt tccatatgaa
      601 agacaaccgc tgggtgcctg gctggatgtg ttcaccacgt ccacagacgg atgccactga
      661 acgcgccgat aacctggctg atgccgcctg atgaacaacc acaccgcccc gccggagacg
      721 gggcggcaga gggagatacc gtgatgaaaa ctgaactgac actgaatgcc ctgcagtcca
      781 tgaatgccca ggaatatgaa gaaatccgtg ctgcgggaag cgatatgcgc cgtaatctca
      841 ctcacgaggt gatgcgtgaa gtggacgcac cggctaactg gatgatgaac ggagagtatg
      901 gcagcgagtt cgggggcttt ttccccgtcc aggttcgttt cacgccagcc cacgaacgtt
      961 tccacctggc attatgttcg ccgggagacg tctctcagct ctggatgctg gttctggtga
     1021 atggtggtgg acagcctttc gccgtcgttc aggtgcaaca tatcttcacg cctgtcgcga
     1081 tcagtcacac gctggcgctt gccgcgacac tggatgcgca gggatacagt gttaacgaca
     1141 tcatccatat cctgatggta gaaggaggtc aggcatgagc gcacgttcac gggcactgat
     1201 ccccctcagc gcagaacaac aggccgccat gcaggcggtg gctgtcacag aacaacgtcg
     1261 tcgtcaggga cgcacacttt cagcatggcc ttatgccacc gctttctttc gctgcctgaa
     1321 tggcagtcgc cggatttcgc tgaccgatct ccgctttttt gcccctgcgc tgacgaagga
     1381 ggaatttcat ggcaaccgtc tcctgtggct ggctgccgtg gataaactga ttgaaagttt
     1441 tggggaagtc tgtgttcttc ccctgccatc cgatgcgggg caccgtctgt tcccgtccgt
     1501 gccttttcgt gaaggtgagc gtcgtcgtca gaaaaccacg ctgacagagc agaaatacag
     1561 ccgccagcgg gaacgtgagg cagaacgacg ggaactggaa taccagacat gttttgctca
     1621 ggcgcagatt gaccttgcgt ttcatactcc cgccacggtc ggaagctggt tgtcccgctg
     1681 gtctggtgtt gttgaggagc atgatctgga aacgattttc tgggggtggt gcgggcgttt
     1741 tccatcactg tcatcatttg accggttttt ctggcaggag gaaccactct ggcggctgat
     1801 ttttgaagcc ggtgaggccg gtcgtggtgc accggtacag gtacgtgcac ttgagcagtg
     1861 gatgatcccg aacaagctgg agaacgtaat atgatgaaat cagacgaaaa ataccaggtt
     1921 cccgcgtgga tgcgacctct gttgccgttg ctctgcaaca ccggggggaa cgatccggaa
     1981 gaactgctga atgatacaga aaccactgcc agtgcgaatg ttgtccgtta tgtactgata
     2041 gttgctgtgc ggtcgcaggt tgatctgctg cagcttctgt acaggaaagg gctgttgcgc
     2101 acagagatac caggtggctt ttcaccggaa gaagcgcagg cactcctgga taatctggtg
     2161 cgcagccata tcagcaaggc gctgtcgggc gagcgaatgg cagcccgtga cagaaatgcc
     2221 gatctggcct ggattggaca gcaactggtc gatgccgcct ggtttgtccg tgccacactg
     2281 gaagcgcatg ggatgagcgt cggaaatgag agtccccctg ctccgccgga gacaatgccg
     2341 gacatacaga cacgggaact ggtgatgttg atcaagcgac tggcatcatc gctgaaagcg
     2401 gtgaaaccgg acagttgtgt ggtgcgtgaa gcgcaggact ggctgcgcga cagaaaactt
     2461 gtggatatca cagatattct ccggtgaacc aggcatgtat attttacgca gggacacgcc
     2521 gatcatgatc gtttcatatc aggggtggtc tggaggtcat gcggcgtgtc ctctgcactc
     2581 gccggaataa ggaagtcgcc ggcggctccg cttttacccg gccatgcggg gcatggcctt
     2641 gtgggttttc agctctgtgg cctcagcgtc gtgtgcgggc tgtgccgtgc ctccatctta
     2701 gccgggctgg cagggatgca agggtacgct tcgccgctgc ggtcacccgg tccctccttc
     2761 ccgtctgccg tgattttccg gtcgttcacc cgctcagatt tcgcggtctc accctccaac
     2821 tcccgccagc cgtgtgcgtc aggctgcggc ttcccttgcg ccctgtgcat ccccgcctta
     2881 tcgcccggct tttatggagg cacggcaccg cccggtgtcg cggaatatgt gaacaacgga
     2941 ggaaaacatc atgcaatatg cgaaacctgt cactctgaac gttgaagagt gcgaccgtct
     3001 ctcctttcta ccgtatctgt ttggcaacga ttttctgtat gccgaggcgt atgtgtacgc
     3061 gctggcgcaa aaaatgatgc cggaatatca gggaggcttc tggcacttca tccgcctgcc
     3121 ggacggtggc ggttacatga tgccggatgg cgaccgtttc cacatggtga acggtgcaaa
     3181 ctggtttgac cgtaccgtga gtgctgatgc cgcaggcatc attctgactt cccttgtgat
     3241 taaccgccag ttgtggctgt accacgacag cggggatgca ggactgaccc agctttaccg
     3301 gatgcgcgat gcgcagttgt ggcgtcacat cgaatttcac cccgaatgca acgcgattta
     3361 cgcggcactg gactgattaa cggacggggc ggcaatgccg cccctgtaaa ggagacgaga
     3421 acatgtactg tactgttaaa gaaattatcc gcgaagtgct gaacacagac gtgccggaca
     3481 gtgaatgcgt ttttgccgtg gtgctgaccc gtggggatgt gcgtcacata gcccaggact
     3541 ggagtctgac agacgatgag ctggaaaccg tcatgcagcg actggacgat gcctttgcgc
     3601 atggtgcgga tgtcagcatt gttcacgacg ttgttcgtga gctgatggaa gaaaagcgcg
     3661 ccagccgtca ggtgacagtt ccggcggtga tgctggaaaa agtgatggcg ctggcaggca
     3721 gtgaaatgaa gcgcctgtat gccgtcggga gcgagaacgg gggcgacggt gacgcattcg
     3781 tcagggaaga acgcgaagca atggacgttg tgttacaggc gctggacggg gagcacatgt
     3841 catgaatatc agcacagaaa cccgcgaaat tctgcgcaat tacaaagccg tgattaatgc
     3901 gcggcgtcgt gaaatggggc agaaaccgct caccactgcg cagattgttg atgaaatctg
     3961 cgattttgtg gcgaatcagc aggctgtatt cctcggcggt cactacatcc ttcagggcag
     4021 cataaacagg tgatacaggg gcaggcggtg gaggccgcct gtcagcccgc gcgaaaaccc
     4081 ctggcaggct gacgcctgcc ggaagggaag cgggccacgc tggcgcgtcg cccgttaccc
     4141 ttcccgtctg tgatgtgtct gactcaggag tgtttctttc ttatgttatg gaactgttcc
     4201 tgatgttgtt gttcctttat ctactgctct gattctgact actgcagtgt tgttctgtct
     4261 gccgggcgca ccttcggctc ccggctgcct ggcggcatcc ggtcgtcagg cccgactccg
     4321 acaggcagct gctggcgcac ctgccagtct ccgccgaacc cgctggcgcg gtttcgggct
     4381 gccgctacgg tccgatgcgt gaaaacccct ggcttgcagc gaccgttccg gtcgcgccgc
     4441 ctctgtcgcg ccgtggcctg cgctcccggc atcccggcaa aaggccgccg ggatgtaagg
     4501 ggcaacgccg tttcgcgcac gcgctctccg tcgttgcggc gggcccctgc tcattgcctg
     4561 ccgggtgcca cacacccgta tgagtccctg tcgccggggg ctgatacagc cacaacctgc
     4621 cgcactgact gtccagggta aatgaacagc ccacaaaatt ttttacccaa aacccggtaa
     4681 aaaattttgc gttctgccca ggacaggtgc gtcaggccgt ggcagtgatg ccccttgcgg
     4741 cgaaggttct caaacgggtg aagttcatac cacagtgaat acagcagcag gttcccgcga
     4801 aacgtcaaaa ccgcggctcc cgaaactcaa cgggtgatgt tctttaaaaa tcaggattta
     4861 tttcaggctc cggcaatacg catttatttg ttctgtcagt catccggctg acaggtaatg
     4921 agaacgcagg aacagaagga ggaaaacacc ggaaagcgtc agatgcagat atgaaaaaag
     4981 caggtcaggc acctgcttaa tccacgccgt aagaattgtg cgaccaattt tgacggcatt
     5041 gttaaccaat ttgtcataag gaatccattt atgactgtaa gcattgtttc accttccgca
     5101 gcggctgtaa agccccgccg tcatccccgt ttcagacgcg aagacatccc cacgccggaa
     5161 attgaccccg tgttgaaagc cttcgggcga cacatagccc ggagttttca ccgggggcgt
     5221 ggtgtacaca tccccgccat gaaaaacacg gctttcgggc aggtgctccg cacactcgaa
     5281 ctgaaacgcg cttttaactg atgaggtgag ccgccccgcg aggggcggca acagacggaa
     5341 ggagaacatt ttatgacatt agcagcaatc accatgaccg ccccggaagc cgccagccct
     5401 gtgcagatgt accgcgcgac ctactcaccg gatgacaaca aactgcgcct gtatgccgcg
     5461 tcacgtcttg acccggagac gtataaaaaa gtgcatgatg ccggttttcg ctgggcaccc
     5521 aaacaggcgc tgtttgtcgc gccggcctgg acaccgggcc gggaagacgt gctcctctca
     5581 cttgccggag agattgagga tgaagacagc acgctcgctg aacgtcagga agcacgggcg
     5641 gagcggttta ccggatacag cggaaagcgg gccagtgaat ccgcacaggc acttgatgaa
     5701 gtggaaagac tggccgcgat gatcccgccc ggtcagccca ttcttgtggg gcatcacagc
     5761 gaacgccgcg cccgtcgtga tgcgcagcgt attgaaaacg gcatgaaacg tgccgtgatg
     5821 ctctttgaac gtgcggaata ctgggaggag cgggcgcggt cggcactgct tcacgcgaag
     5881 tataaagaac gcccggacgt tcgctggcgt cgtatcaaaa aaatcgaagc ggatttgcgc
     5941 aaggctgaaa agaccatcgc acagtcgcag aaatatctga cgatgtggcg ggctgaatcg
     6001 ctggatctga atatggcaaa actcatcagc agtcatgacc atatcagcgc ctgtttcccg
     6061 ctggatacgt atccgcgccc ggcagaaaaa agccagtatg aagggagccg gtcgttatgg
     6121 tcggccctgg atgatgacat catcaccacg gagcaggccc gcgaaattgc gatccgctgt
     6181 catgaacggc agattcagca tcagcaacgc tgggttaacc actatcagaa ccgcctgatc
     6241 tatgagcgtg ccatgctgga cgaaagcggc ggcgtggtta cccggacaca ggattttgag
     6301 ccgggcggac aggttttcag ccggggcgag tggctgacta tcatccgcgt gaacaaaagc
     6361 aacggggcgg tgagttcagt cacaacgcca aattacagtt ttctcgggta cagcggcacg
     6421 atgaaagtga cgcccgatcg catcacggac tacaaagcac catcggcaga agaggctgcc
     6481 gtcgccagtc aggccgcgaa gcgtccgccg gtagtcaact atccggggga aggtttccgg
     6541 gaaatgacaa aggcgcagtg ggccgccctg ccccgggact gtaaggccgt gcgcagtgtg
     6601 gaagaagcag aagaccacgg ggcataccgc taccgccgca caatggacaa taatttccgt
     6661 ctggtgaatg tgtatatcac cgacatgaaa atcacggaaa tcccgcagaa ataaggtata
     6721 tccccccggg aaatccccgg gggatgacgg agataatgac atgcacagtc agttaagaga
     6781 acgtatccgg ctgatgcgcg caaggctgga taacgccgcg ccggttgctg aaatccgggc
     6841 tgaatctcag ctttttgtga ctcctgcccc ggtctgtgat cgcctggtga cgctggcgga
     6901 aatcagcaac cgtgatcaca ttctggaacc ctctgccggc accggggcca tactgcgggc
     6961 gattcgggat accgcgccgg aggccatgtg tgatgcggtg gagattaaca gcgggctggt
     7021 ccggtatctg cgggaaaact ttaacggtgt cagggtacag tgtggcgact tcatggaatg
     7081 gcagccggtg cagtattaca gccggatcat tatgaatccg ccgttcagtc acgggcagga
     7141 tatccggcat atcctgcggg ctttttccct gttgcgtccg gggggcgttc tggttgccgt
     7201 ctgtctgaac gggccgcgcc agcaggagaa gctgttaccg ttttctgatg tccgcgaggt
     7261 gctgccgtgc ggcacatttg cttatacccg tgttccgacg atgattattc gtctgcgtgc
     7321 ctgatgaatc cgggcaggcg ggaggataaa tgcccgtctg cccgcgccgg gaaaagaccg
     7381 cgcgccggtg gcgctgaaag gggccgcgct tacgcgccgc ccctttcatt acgcggacgt
     7441 tctgctgtgg gcttctgtct tttcagacct gtcatttgcc tgttactgat gatgttgttg
     7501 ttcctgctac tgctactgct actgctactg ctactgttct tcttttttct gctgctctga
     7561 tta
//
LOCUS       DAZPAC010000200         7493 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZPAC010000200 DAZPAC010000000
VERSION     DAZPAC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.201"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1554
                     /gene="lpfC-O113"
                     /locus_tag="ACHUPM_003536"
     CDS             <1..1554
                     /gene="lpfC-O113"
                     /locus_tag="ACHUPM_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR0871217.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
     gene            1565..2638
                     /gene="lpfD-O113"
                     /locus_tag="ACHUPM_003537"
     CDS             1565..2638
                     /gene="lpfD-O113"
                     /locus_tag="ACHUPM_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR0871218.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            2886..3926
                     /gene="pstS"
                     /locus_tag="ACHUPM_003538"
     CDS             2886..3926
                     /gene="pstS"
                     /locus_tag="ACHUPM_003538"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR0871219.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            4013..4972
                     /gene="pstC"
                     /locus_tag="ACHUPM_003539"
     CDS             4013..4972
                     /gene="pstC"
                     /locus_tag="ACHUPM_003539"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR0871220.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            4972..5862
                     /gene="pstA"
                     /locus_tag="ACHUPM_003540"
     CDS             4972..5862
                     /gene="pstA"
                     /locus_tag="ACHUPM_003540"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR0871221.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            5953..6726
                     /gene="pstB"
                     /locus_tag="ACHUPM_003541"
     CDS             5953..6726
                     /gene="pstB"
                     /locus_tag="ACHUPM_003541"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR0871222.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            6741..7466
                     /gene="phoU"
                     /locus_tag="ACHUPM_003542"
     CDS             6741..7466
                     /gene="phoU"
                     /locus_tag="ACHUPM_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR0871223.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1898 a         1740 c         1947 g         1908 t
ORIGIN      
        1 gtaaccatca aagaagaaga cggtagcgaa caacgattca cccaacctta tgcttcatta
       61 gcgattctta aacgtgaagg tcagacagat gttgatgtca gcgtgggtga attgcgcgat
      121 gaagacggat ttacaccgga cgtccttcag gcgcaaatac ttcatggttt ttcccacggg
      181 atcactttat atggaggtat gcaggctgct gaaaattatg gttctgcagc tctgggtgtc
      241 ggtaaagatc ttggcgcttt gggcgcaatt tctttcgatg tgacacatgc tcgtgcgaat
      301 tttagccatg atgatacaga aacgggtcag tcatatcgct ttctctattc aaaacgattt
      361 gacgacacag acactagctt gcgcttggtt ggctatcgtt actccaccga gggctactat
      421 accctcaatg agtgggcatc gcggcgcaac agccctgaag acttttggga aacaggtaac
      481 cgacgtagtt gcgtggaggg gacgctaacg cagtcgttgg ggagagatta tggcaattta
      541 tacctgacat taagccggca acaatactgg cataccgatg atgtcgaacg attaatgcaa
      601 tttggctaca gcagtagctg gaagcgtctc tcgtggaacg tctcctggag ttattccaat
      661 actgccagac aggggacggg gaacaaccat gccagtgata acaccagtga gcagatctac
      721 atgctctctt tatctgttcc tttattgggc tggtggggta atagttacgc cacctattct
      781 gtttcgcaaa acgataattc cggtagctca catcaactcg gactcagcgg tacggcgctg
      841 gaaagaaata acctttcatg gaatttaatg cagtcctata acagtcatga tgatgaggtt
      901 ggcggtaata tgtccctgac ctatgatggc tcttatggca cggtgaacgg cagctataac
      961 tacagccaaa attcccagag gctgaattat ggtatcagag ggggaattct ggcacacagc
     1021 gaaggggtaa cgttaagtca ggagttaggt gaaactattg ctcttgttaa agcacctggg
     1081 gccgccgggt tagaaataga taatatgcgc ggtgctgcga cggactggcg cggctatacg
     1141 gtcaagacac agctaaaccc ttatgatgaa aatcgggtag caatcagcga taactatttc
     1201 tcgaagtcga atatagaact tgataatacc gtcgttacga tggttcccac gcgtggtgca
     1261 gtggttaaag cggagtttgt gactcatgtg ggttatcgcg ttctcttcag agtgttaaat
     1321 gcaaatggta aaccggtacc ttttggagcc attgctgcga tacaagatgc aagtttggca
     1381 gattcaggaa ttgtcggtga ccgtggcgaa ctttatcttt ctggtctacc agaaaaagga
     1441 caggttacgt tatcctgggg agaaaacgcc tcaacaaaat gcatcttcaa ttattcactt
     1501 tcgacaccag aaagtgagag cggattaatt gaacagggtg tgacatgtca ttaaaaggat
     1561 ttttatgaac aagtatatca aacagtggtg ctttgctgtg tttatgctct cgttaagtag
     1621 tgtagcactt gcagctccta aaggtatctg tacccctgat aatggcgtat ttcaccgtac
     1681 gcttgatttc tcgggatatc ttattaccgc gaacgaaaat aaggttggaa cgacatttaa
     1741 taccactgta actaatggat cctcatatcc tggacgctgt cattgtgata ctggtaatgt
     1801 aggagaattc ccctatatct attatacgtc aaaaataaac caggcattaa cttatgctgg
     1861 ggttcactct aatattaatt attatgatct taatccgaac cttgatgttg ggattgctat
     1921 agatattctt ggggttgggt atgtgaatgc accttttgaa tatcatgcta acaacccgtc
     1981 tggtaataca aagtacaatt gcaaccgcat tgaaccttta agtatatcca gtgggggtaa
     2041 agcgatagta tatttttata tcaagaagac atttgcagga aaattgatta ttcctgaaac
     2101 aaagatagtg acattgtatg gaacaattag ccgtgacacc ccggtggatt actcacagcc
     2161 gatggctgat gtttatattc ggggcgatat tacagctccg caaagttgcg aaataaataa
     2221 tttacagcca gtttattttg attttaaaga aataccagct gcagattttt catctgttgt
     2281 tggaagtgcg gtaacaacac ataaaattac taaaacagtc actattgagt gtgaaaacct
     2341 ggggatactg aataccgatg atatcagtac gtctttttat gctaccgaac ccaatacgga
     2401 caattcaatg gtcgtaactt caaactctaa cgtggggata aaaatttacg acaaaaataa
     2461 taaggaaatc aaagtgaacg gtggtgagtt gccaacagac atgggtaaat caacagtgta
     2521 tggtgaaaaa tcaggtagtg taactttttc agctgctcct gcgagcctta ctggagctcg
     2581 cccagccccg gggcaattta ctgcaacagc gacgataact gttgagattg tgcgctaata
     2641 ggtctgacaa cgattgttca tcacatcgtt gtcatctcac ccatttcttt atcactttta
     2701 aatgcatcat gacaaaaaga tgacatgtgg gattttcatc ttcacattta aaatcagtaa
     2761 gttatttgtt aattcctttc tgtcataaaa atgtcatatt ccttacatat aactgtcacc
     2821 tgtttgtcct attttgcttc tcgtagccaa caaacaatgc tttatgaatc ctcccaggag
     2881 acattatgaa agttatgcgt accaccgtcg caactgttgt cgccgcgacc ttatcgatga
     2941 gcgctttctc tgtgtttgca gaagcaagcc tgacaggtgc aggtgcaacc ttccctgcgc
     3001 cggtgtatgc caaatgggct gacacttacc agaaagaaat cggtaataaa gttaactacc
     3061 agggtatcgg ttcttccggt ggcgtaaaac agattatcgc taataccgtt gattttggtg
     3121 cctctgactc gccgctgtct gacgaaaaac tggctcagga aggtctgttc cagttcccga
     3181 ccgtgattgg cggcgtggtg ctggcggtta acattccagg gctgaagtct ggcgaactgg
     3241 tgctggatgg taaaaccctc ggcgacatct acctgggcaa aatcaagaag tgggatgatg
     3301 aaaccatcgc caaactgaat ccgggtctga aactgccttc acaaaacatt gctgtagtac
     3361 gccgcgcaga tggctccggg actaccttcg tcttcaccag ctacctggcg aaagtgaacg
     3421 aagagtggaa aaacaacgtt ggtactggct ctaccgtaaa atggccgatc ggtctgggcg
     3481 gtaaaggtaa cgacggtatc gccgcgttcg ttcagcgtct gccgggtgca attggttatg
     3541 ttgaatatgc ttacgcgaag cagaacaacc tggcgtacac caaactgatc tccgctgatg
     3601 gtaaaccggt tagtccgacc gaagaaaact tcgctaatgc agcaaaaggt gcagactgga
     3661 gcaaaacctt cgctcaggat ctgaccaacc agaaaggcga agatgcatgg cctattacct
     3721 ctaccacgtt cattctgatc cacaaagatc agaagaaacc agaacaaggc acagaagtgc
     3781 tgaaattctt cgactgggcg tacaaaaccg gggctaaaca ggcgaacgac ctggattacg
     3841 ccagcctgcc ggatagtgta gttgaacagg ttcgcgctgc gtggaagacc aatattaaag
     3901 acagtagcgg taagccgctg tactaataaa actccaggcc gggtacggtg ttttacgccg
     3961 catccggcat tacaaaatga ctttgtaaac gcgtttaact gaagagtaac ttatggctgc
     4021 aaccaagcct gcttttaacc caccgggtaa aaagggcgac ataattttca gcgtgctggt
     4081 aaaactggcg gcgctgattg tgctattgat gttgggtggc attattgtct ctctgatcat
     4141 ctcctcctgg ccgagcattc agaaatttgg tctggctttc ctatggacca aagagtggga
     4201 tgcaccgaac gatatctacg gggcgctggt gccgatctac ggtacgttgg tgacttcgtt
     4261 tatcgcgctg ctgatcgccg tcccggtgag tttcggtatc gccctgttcc tgactgagct
     4321 tgcgcctggc tggctgaaac gcccgctggg tatcgccatt gagctgctgg cagccattcc
     4381 aagtatcgtt tacggcatgt ggggcctgtt tatctttgcg ccgctgttcg ccgtttactt
     4441 tcaggagccg gtcggcaata tcatgtcgaa tatcccgatt gttggcgcgc tgttctctgg
     4501 ccccgcattt ggtatcggta tcctcgcggc aggcgtgatc ctcgccatca tgattattcc
     4561 gtacattgcg gcggtaatgc gtgatgtgtt cgaacaaacc ccggtgatga tgaaagagtc
     4621 ggcctacggt attggctgca ccacctggga agttatctgg cgtatcgttc ttccgttcac
     4681 caaaaatggt gttatcggcg gcatcatgct ggggctgggc cgcgcgctcg gtgaaaccat
     4741 ggcggtgacc tttatcatcg gtaacaccta ccagctcgac agcgcctcgc tgtacatgcc
     4801 gggcaatagt atcacctctg cgttggcgaa cgaatttgcg gaagcggaat ccggtctgca
     4861 cgttgccgca ctgatggaac tgggcctgat cctgtttgtg attaccttca tcgtcctcgc
     4921 cgcatcgaag tttatgatta tgcgcctggc taagaatgag ggggcacgct aatggctatg
     4981 gttgaaatgc aaaccactgc ggcgctggct gaatctcgcc gcaaaatgca ggcgcgtcgc
     5041 cgcctcaaaa accgtattgc gctgacgctc tcgatggcga cgatggcctt cggcctgttc
     5101 tggctgatct ggattttaat gtccaccatc actcgcggta tcgacggtat gtcgctggcg
     5161 ctgttcactg aaatgacgcc gccgcccaat acggaaggtg gtggtctggc gaacgccctg
     5221 gcgggtagcg ggctgttaat tttgtgggcc acggtattcg gtacgccgct gggcattatg
     5281 gcggggattt atctggcgga atatggtcgt aaatcctggc tggcagaagt gattcgcttc
     5341 attaacgaca ttctgctctc cgtgccgtcg attgtggttg gtctgtttgt ctacaccatc
     5401 gtggtggcac agatggagca cttctccggc tgggcgggcg taattgcgct ggcgctgttg
     5461 caggtgccga tcgttatccg caccaccgag aacatgctga aactggtgcc ggacagcctg
     5521 cgtgaagcgg cttatgcgct gggtacaccg aagtggaaga tgatctccgc gattacgctg
     5581 aaagcgtcgg tgtccgggat tatgaccggt atcctgctgg cgattgcccg tattgccggt
     5641 gaaaccgcgc cgctgctgtt taccgcgctc tccaaccagt tctggagcac ggacatgatg
     5701 cagccgatcg ccaacctgcc ggtgacgatc tttaagtttg cgatgagccc gtttgcggaa
     5761 tggcaacaac tggcctgggc cggggtattg atcattaccc tgtgcgtcct gctgctgaac
     5821 attctggcgc gcgttgtttt tgcgaagaat aaacacggtt aacactttgc cggatgcggc
     5881 gtgaacgcct tacccggcct acggtaagcc tgattagcga aaagcatcag gtacgatgag
     5941 gaaaagattg caatgagtat ggttgaaact gccccgagta aaattcaggt tcgtaatttg
     6001 aacttctact acggcaaatt ccatgccctg aaaaacatca acctggatat cgctaaaaac
     6061 caggtaacgg cgtttatcgg gccgtccggc tgcggtaaat cgacgctgct gcgtaccttc
     6121 aacaaaatgt ttgaactgta tccggagcag cgcgcagaag gtgaaattct gcttgatggc
     6181 gacaacatcc tgaccaactc tcaggatatc gcactgctgc gtgcgaaagt gggcatggtg
     6241 ttccagaaac cgacgccgtt cccgatgtcc atctacgaca acatcgcttt tggtgttcgt
     6301 ctgtttgaga agctctcccg tgccgacatg gacgagcgcg tgcagtgggc attgaccaaa
     6361 gccgcattgt ggaacgaaac caaagataaa ttgcaccaga gcggttactc tctctctggt
     6421 ggtcagcaac agcgtctgtg tattgcgcgt ggtatcgcca ttcgcccgga agtgctgctg
     6481 ctcgacgaac cgtgttcggc gctcgacccc atctctaccg ggcgtattga agagctgatc
     6541 accgaactga agcaggatta caccgtggtg atcgttaccc acaacatgca gcaggctgcg
     6601 cgttgttccg accacacggc gtttatgtac ctgggcgaat tgattgagtt cagcaacacg
     6661 gacgatctgt tcaccaagcc agcgaagaaa caaacagaag actacatcac cggtcgttac
     6721 ggttaattca ggagtgcgtt atggacagtc tcaatcttaa taaacatatt tccggccagt
     6781 tcaacgccga actggaaagt atccgcacgc aggtgatgac catgggtggc atggtggagc
     6841 agcagctttc tgatgcaatc accgcgatgc ataaccagga cagcgatctg gcgaagcgcg
     6901 tgatcgaagg cgacaagaac gtcaacatga tggaagtggc gatcgatgaa gcctgcgtgc
     6961 gcattatcgc caaacgtcag ccgacggcga gcgacctgcg actggttatg gtgatcagta
     7021 aaaccattgc cgagctggag cgtattggcg acgtggcgga caaaatctgc cgtactgcgc
     7081 tggagaaatt ctcccagcag catcagccgt tgctggtaag tctggagtcg ctgggccgtc
     7141 ataccatcca gatgctgcac gacgtgctgg acgcgttcgc gcggatggac attgacgaag
     7201 cggtacgtat ttatcgtgaa gataaaaaag tcgatcagga atacgaaggt attgttcgtc
     7261 aactgatgac ctacatgatg gaagattcgc gtaccattcc gagcgtactt actgcgctgt
     7321 tctgcgcgcg ttctatcgaa cgtattggcg accgctgcca gaatatttgt gagtttatct
     7381 tctactacgt gaaggggcag gatttccgtc atgtaggcgg tgatgagctg gataaactgc
     7441 tggcgggaaa agatagcgac aaataattca ccagacaaat cccaataact taa
//
LOCUS       DAZPAC010000201         7491 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZPAC010000201 DAZPAC010000000
VERSION     DAZPAC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.202"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHUPM_003543"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHUPM_003543"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFR0871224.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVTYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="ACHUPM_003544"
     tRNA            647..722
                     /locus_tag="ACHUPM_003544"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHUPM_003545"
                     /gene_synonym="yqjH"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHUPM_003545"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFR0871225.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHUPM_003546"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHUPM_003546"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR0871226.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHUPM_003547"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHUPM_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFR0871227.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHUPM_003548"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHUPM_003548"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR0871228.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="ACHUPM_003549"
     CDS             complement(5917..6249)
                     /locus_tag="ACHUPM_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFR0871229.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHUPM_003550"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHUPM_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR0871230.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1824 a         2029 c         1869 g         1769 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat gtcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaatacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacag tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgca
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAZPAC010000202         7487 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZPAC010000202 DAZPAC010000000
VERSION     DAZPAC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.203"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1049
                     /gene="dgcN"
                     /locus_tag="ACHUPM_003551"
     CDS             <1..1049
                     /gene="dgcN"
                     /locus_tag="ACHUPM_003551"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFR0871231.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
     gene            1065..1547
                     /locus_tag="ACHUPM_003552"
     CDS             1065..1547
                     /locus_tag="ACHUPM_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR0871232.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(1624..1971)
                     /gene="rplS"
                     /locus_tag="ACHUPM_003553"
     CDS             complement(1624..1971)
                     /gene="rplS"
                     /locus_tag="ACHUPM_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR0871233.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2013..2780)
                     /gene="trmD"
                     /locus_tag="ACHUPM_003554"
     CDS             complement(2013..2780)
                     /gene="trmD"
                     /locus_tag="ACHUPM_003554"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR0871234.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(2811..3359)
                     /gene="rimM"
                     /locus_tag="ACHUPM_003555"
     CDS             complement(2811..3359)
                     /gene="rimM"
                     /locus_tag="ACHUPM_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR0871235.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(3378..3626)
                     /gene="rpsP"
                     /locus_tag="ACHUPM_003556"
     CDS             complement(3378..3626)
                     /gene="rpsP"
                     /locus_tag="ACHUPM_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR0871236.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(3875..5236)
                     /gene="ffh"
                     /locus_tag="ACHUPM_003557"
     CDS             complement(3875..5236)
                     /gene="ffh"
                     /locus_tag="ACHUPM_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR0871237.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            5403..6194
                     /locus_tag="ACHUPM_003558"
     CDS             5403..6194
                     /locus_tag="ACHUPM_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR0871238.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6215..>7487
                     /locus_tag="ACHUPM_003559"
     CDS             6215..>7487
                     /locus_tag="ACHUPM_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR0871239.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRE"
BASE COUNT         1778 a         2033 c         1817 g         1859 t
ORIGIN      
        1 tgacttacag tctggaagca gctgtcgttt ttgccgatgg ccctgcagca actgaaacac
       61 tggcagcgct gggccagcaa gggcaatttt caactgcaga agtacgtgat aagcagcaaa
      121 atattctggc gtcctggcat tacacccgta aggatccagg cgatactttc agcaatttca
      181 taagccactg gctcttcccc gcccccatca ttcagccgat tcgtcacaat ggtgaaacca
      241 ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag ccattttatc tggttttcgc
      301 tcgccgtact gaccggttgt attctgctgg catcaggcat cgcaattacc ctcacccgcc
      361 atttgcacaa tggcctggtg gaagcactga aaaatatcac cgatgtcgta catgatgtgc
      421 gttccaaccg caatttttcc cgacgagttt cggaagaacg tatcgctgag tttcaccgct
      481 tcgctctcga cttcaacagt ctgctggatg aaatggaaga gtggcagctt cgtttacagg
      541 ctaaaaatgc gcagcttcta cgtaccgcgc tacatgaccc attaaccggg ctggctaacc
      601 gcgcagcgtt tcgtagcggc atcaacacgt tgatgaacaa ttccgatgcc cgaaaaacgt
      661 cggcgttact atttcttgat ggcgataatt tcaaatacat caatgatacc tggggtcatg
      721 cgacgggcga tagagtcttg attgaaatcg caaaacggtt agctgaagtt ggcgggctgc
      781 gacataaagc ataccgcctg ggcggcgatg aattcgctat ggtgctctat gatgtacagt
      841 cagaatctga agtgcagcag atatgctcag cactgacaca aatctttaat ctcccgtttg
      901 atcttcataa tggccatcag accaccatga cattaagcat tggttacgcg atgaccattg
      961 agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca caatatgtat caggccaaac
     1021 accagcgtgc cgaaaagctg gtgagataac aaggatatat cgatatgata aagcacctag
     1081 tagcacccct gattttcacc tcactaatac tgactggctg ccagtcccct cagggaaagt
     1141 ttactcctga gcaagtcgcc gctatgcaat cttatggatt tactgaatcc gccggcgact
     1201 ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa tgactacaaa ttgctcccgg
     1261 aaagccagca acagatccaa accatggcag ccaaattggc ctcgacaggg ctaacacatg
     1321 cccgtatgga tggacacacc gataactatg gtgaagacag ttacaacgaa ggcttatcat
     1381 tgaaacgggc gaatgtcgtg gccgatgcat gggctattgg tggacaaatt ccacgcagca
     1441 atctcaccac acagggttta ggaaaaaaat atcccatagc cagtaacaag accgcccagg
     1501 gccgcgccga gaaccgccgc gtcgcagtgg tgattacaac cccttaaaac acgagcaaac
     1561 agacaaaaaa agggccagcc aattggccag cccttcttaa caggatgtcg cttaagcgaa
     1621 atcttagtta agacgctctt tgatacgagc agccttacca gtacgctcac gcaggtagta
     1681 cagtttagct ttacgaacag caccacgacg tttgacagaa atgctgtcaa ctaccggaga
     1741 gtgagtctgg aagacacgct caacgccttc gccgttggaa attttacgaa cagtgaatgc
     1801 agagtgcaga ccgcggttac gaatagcgat aaccacgccc tcgaatgcct gcagacgttt
     1861 tttggaacct tcaacaaccc atactttcac ttccacggta tcacccggac ggaaggaagg
     1921 tacgtcctgc ttcatctgct cttgttcaag ttgcttaata atattgctca taatttaatc
     1981 tcttatcctg ggtaaactga tatctcgggg gcttacgcca tcccatcatg tttatgttgc
     2041 tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg cttgctcttc agtcagagcc
     2101 aggttttcca gaagttcagg tcttctaagc caggtacggc ccagcgactg tttcaaacgc
     2161 cagcgacgta tctcggcatg gttgcccgac agtaacactg gcggaacttc catcccttct
     2221 aacacctcag gccgcgtata gtgcgggcaa tccagcaatc cttcagcaaa ggaatcttcc
     2281 gttgccgagg cttcatgtcc cagtactccc ggaataaacc gggaaacgga gtcaatcagc
     2341 gtcattgctg gtaactcacc accactgaga acgtaatcgc cgattgacca ttcttcgtca
     2401 atttcggttt ggatcacgcg ctcatctata ccttcgtagc gaccgcacac cagaatcaat
     2461 ttttgattcg ttgccagttc gctgacgccc gcttgatcaa gcttgcgtcc ctgtggtgac
     2521 agataaatca cctttgcgcc ttcacccgcc gcggcttttg ctgcatgaat ggcgtcccgc
     2581 aagggttgca ccatcattaa catccccggt ccgccaccgt aaggacgatc gtccacggta
     2641 cggtgccggt catgcgtgaa gtcgcgagga ctccaactct ggatgctcag caggccattt
     2701 ttaactgccc ggccagttac cccgtaatcg gtaattgcgc ggaacatttc aggaaacagg
     2761 ctaattatgc caatccacat agcgccgtct tttaccgttt atccggtggt ttaaaaacca
     2821 ggatcccaat ctacttcgat tgaacgagta gtgagatcga ctttcttgat aacctgccca
     2881 tcgaggaacg gtacgagacg ttccttgata ccaaacgcat ctttcaggtt tgccttaatg
     2941 acgagaacgt cattagatcc ggtttccatc atatcgacga ctttaccgag atcgtagcct
     3001 tcagtggtta ctacctggca gcccatcagg tctttccagt agtagtcccc ctcttcaagc
     3061 tgaggcagct gcgatgaatc cacgacaatt tcacaattcg tcagcaggtt cgccgcatca
     3121 cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat tgtggtgctt ccagctttcc
     3181 agctggactt gctgccactg acccgccttc tggataaacc agggctgata gtcaaaaatg
     3241 ctttcggcgt cttcggtgga agaaaacact ctgagccacc cacgaatacc gtaagacgaa
     3301 cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg cggtgagttg tttgctcatc
     3361 atgaccaccg tgacagatta agctgctttg tttacttctt tgatcagcgc agcaacgcga
     3421 tcagaaatag ttgcgccctg gccaacccag tgagcgatgc gatccagatc caggcgagtg
     3481 ccttcttctt tttcgctagc gattgggttg aagaaaccaa cgcgctcgat gaagcgaccg
     3541 ttgcgtgcat tacggctgtc agcgacaaca acctggtaga acggacgctt tttagcgccg
     3601 tgacgtgcta aacgaatagt taccataaca tcctcttgtg tgaataaaac aaccggaccc
     3661 catcgaggaa cggagtccgg tgtcatatta aaagcccgaa aattttactc atttttgcgg
     3721 gaattgcaat caacagttgc taactctgct gtaaaaggcc gtcggcggtg cagccagttt
     3781 ggtgccggag tgcgcgcagt caccggagcg tacacgcagt acgtgaggat gacgagcaca
     3841 tcccggtgcc aaaatggcaa acaagccagg ccgattagcg accagggaag cctgggggca
     3901 tcataccctt catgcttctc atcatcttcg ccattccgcc cttcttcatt ttcttcatca
     3961 tgcgctgcat gtcgtcgaac tgcttcagaa ggcggttaac gtcctgcacc tgcataccgc
     4021 aaccggcagc gatacgacgc ttacgcgaac ctttgatgat ttctggctta gcgcgctctt
     4081 tcatcgtcat cgagttgatg atggcttcca tacgcaccag cactttatcg tccatctgcg
     4141 acttaacatt atccgggatc tgccccatgc ccggcagctt gcccatcagg ctagccatgc
     4201 cgcccatatt tttcatctgg cgcagctgct caagaaaatc gttgagatcg aagccgtcgc
     4261 cttttttcag cttgctggct aatttctctg cctgcgcgcg gtcaactttg ctttcgatat
     4321 cttcgatcag cgacagtacg tcgcccatgc cgagaatacg cgacgcgatg cggtccggat
     4381 ggaacggctc cagcgcctca gttttctcgc caacaccgag aaacttgatc ggtttgccag
     4441 tgatgtgacg aatagagagc gccgcaccgc cgcgggcatc gccgtccact ttggtcaaca
     4501 ctacgccggt aagcggtaac gcttcattga atgcttttgc cgtattggcc gcatcctgac
     4561 cggtcatggc gtcaaccaca aacagggttt caaccgggtt aatcgacgca tggacttgtt
     4621 tgatctcgtc catcatcgct tcgtcaacgt gcagacgacc agcggtatcc accagcagca
     4681 cgtcgtagaa tttcagtttg gcttctttca gcgccgcgtt aacgatatct accggcttct
     4741 gaccaacatc agaagggaag aaatcaacgc ccacctgctc tgccagcgtc tcaagctgtt
     4801 tgattgccgc cgggcgataa acgtcggcag aaaccaccag cactttcttc ttgtgcttct
     4861 cgcgcaggaa cttaccgagt ttaccaacgc tggttgtttt accggcacct tgcaggcccg
     4921 ccatcagcac gaccgcaggc ggttgcgccg ccaggttcag ggtctggttc tcttcgccca
     4981 tcgccgcaac cagttcgtta cggacgattt tgacgaactc ctgccccggc gtcaggctct
     5041 tattaacttc atgaccaacc gctttctctt ttacgcgatt gataaactca cgcactaccg
     5101 gcagagcgac atccgcctcc agcagcgcca tgcgcacttc gcgcagcgta tcttttacgt
     5161 tgtcttcagt gaggcgtcca cggccactga tattgcgcag cgtgcgcgac aaacgatcgg
     5221 ttaaattatc aaacattgtc tctcgcctgg ggtggaaacg gttggccgca atcgcgacac
     5281 atcatcagta ttttgccgca gtataacatg aaggcgtctt tgttgttatg caacggttgg
     5341 agcagcgttc acctgacgct atactgcttc tctttcttat tgctcaaact gtcgacatca
     5401 ctatgcccgt ttttgctctg ctcgcgcttg tcgcctactc cgtcagtctt gcgctgattg
     5461 ttcccggtct gctgcaaaaa aacggcggct ggcggcgcat ggctattatt tctgcggtca
     5521 ttgcgctggt ctgccacgca atcgctctgg aagcccgcat cctgcccgac ggtgatagcg
     5581 gacaaaacct cagcctgctg aacgttggtt cattggtcag tttgatgatc tgtacggtaa
     5641 tgaccattgt ggcttctcgc aatcgtggct ggttgctgct acccattgtc tatgcctttg
     5701 cgcttatcaa cctggcgctg gcgaccttca tgcccaatga atacatcacc catctggaag
     5761 caacgcctgg gatgctggtg cacattggct tatcgctctt ttcctatgcc acgctaatta
     5821 tcgccgccct gtacgcgctg caactggcgt ggattgatta ccaactgaag aacaagaagc
     5881 tggcatttaa ccaggaaatg ccgccattga tgagtatcga gcgtaaaatg ttccacatca
     5941 cgcagattgg cgtggtgctg ctaacgctca cgctttgcac tggcctgttc tacatgcaca
     6001 acctatttag catggaaaat atcgacaagg ctgtgctctc tatcgtggcg tggtttgtct
     6061 atattgtgct gctgtgggga cattaccatg aaggatggcg tggacgccgc gtcgtctggt
     6121 ttaacgttgc gggcgcggta attctgacac tggcctactt cggcagccga attgtccagc
     6181 agttaatcag ctaaacccag aaaaggagtt tcccctggaa cacatttcta ctactacgtt
     6241 gatcattatt ctgatcatca tggtggtcat ttcagcctat ttttccgggt ccgaaaccgg
     6301 aatgatgacc ctcaaccgct atcgtctgcg acatatggcg aaacagggta atcgctcggc
     6361 caaacgcgtc gaaaaattgc tgcgtaagcc agaccgcctg ataagcctgg tgttaatcgg
     6421 caataacctg gtcaatattc ttgcctccgc gctcggcact attgttggga tgcgtttgta
     6481 cggcgatgcg ggcgtggcaa ttgcgactgg tgtgctgact tttgtcgtac tggtatttgc
     6541 tgaggtattg ccgaaaacca ttgccgcgct gtacccggaa aaagtcgctt atccgagtag
     6601 ttttctgctg gctccgctgc aaattttgat gatgccgctg gtctggttgc tgaatgctat
     6661 cacccgtatg ctgatgcgca tgatgggtat caaaaccgat atcgtggtta gcggctcttt
     6721 gagcaaagaa gagttgcgca ctatcgtgca cgaatcacgc tcacaaattt cccgtcgcaa
     6781 tcaggatatg ctgctgtcgg tgctcgatct ggaaaaaatg accgttgatg acatcatggt
     6841 gccgcgcagt gaaattatcg gtattgatat caacgatgac tggaaatcga ttctgcgcca
     6901 actctcccac tcacctcacg ggcgcatcgt gctctaccgt gattcgctgg acgacgccat
     6961 cagtatgctg cgagtacgtg aagcctggcg gttgatgtcg gagaaaaaag agttcaccaa
     7021 agaaaccatg ctgcgcgccg cggacgagat ctattttgtg ccggaaggta cgccgctcag
     7081 cacgcagttg gtaaagtttc agcgcaacaa aaagaaagtc ggcctggtcg tcaacgagta
     7141 tggagacatt caggggctgg tgacggttga agatattctg gaagagattg tcggcgattt
     7201 cactacgtcg atgtcgccaa cacttgccga agaggtcacg ccgcaaaacg acggttcggt
     7261 gattatcgat ggcaccgcca acgtgcggga aatcaacaaa gcctttaact ggcatctacc
     7321 ggaagatgat gcccgcacgg ttaatggcgt cattcttgag gcactggaag agatccctgt
     7381 cgcaggcacc cgcgtgcgta ttggcgagta cgatatagat attctcgacg tacaggacaa
     7441 tatgattaag caggtaaaag tttttcctgt gaaaccgctg cgcgaga
//
LOCUS       DAZPAC010000203         7453 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZPAC010000203 DAZPAC010000000
VERSION     DAZPAC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.204"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..232
                     /locus_tag="ACHUPM_003560"
     CDS             <1..232
                     /locus_tag="ACHUPM_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR0871240.1"
                     /translation="PAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQEN
                     HPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
     gene            232..5502
                     /gene="traI"
                     /locus_tag="ACHUPM_003561"
     CDS             232..5502
                     /gene="traI"
                     /locus_tag="ACHUPM_003561"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFR0871241.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRTATSEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5522..6268
                     /gene="traX"
                     /locus_tag="ACHUPM_003562"
     CDS             5522..6268
                     /gene="traX"
                     /locus_tag="ACHUPM_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR0871242.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            6323..6883
                     /locus_tag="ACHUPM_003563"
     CDS             6323..6883
                     /locus_tag="ACHUPM_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR0871243.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGETPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            7015..7227
                     /locus_tag="ACHUPM_003564"
     CDS             7015..7227
                     /locus_tag="ACHUPM_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR0871244.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
BASE COUNT         1698 a         1870 c         2485 g         1400 t
ORIGIN      
        1 tccggcaggg gggatcgagc aggagctgaa aatgaaaccg gaagaagaga tggaacagca
       61 actgccaccc gggatcagtg aatccggtga agtggtggat atggccgctt atgaggcatg
      121 gcaacaggaa aatcatccgg acatccagca gcatatgcag cgtcgtgaag aggtgaacat
      181 taatgtgcac cgggagcgcg gggaggatgt tgagccggga gatgatttct gatgttgagt
      241 ttcagcgttg ttaaatcggc aggcagtgcc ggaaactatt acaccgacaa ggataattac
      301 tatgtgctgg gcagcatggg agaacgctgg gccggtcagg gggctgaaca gcttgggctg
      361 cagggcagcg tcgataagga tgtttttacc cgtcttctgg agggcaggct gccggacggg
      421 gcggatttaa gccgcatgca ggatggcagt aataagcatc gtcccggcta cgacctgacc
      481 ttctccgccc ccaaaagtgt ctccatgatg gccatgctcg ggggggataa acgtcttatt
      541 gatgcacata accaggccgt ggattttgct gtccgtcagg tggaggcgct ggcttccaca
      601 cgggtgatga cggacggaca gtcagaaacg gtgctgaccg gtaatctggt gatggcactg
      661 tttaaccatg acaccagtcg cgatcaggac ccacagttac acacgcatgt ggtggtggct
      721 aatgtcacgc agcataacgg cgagtggaag acactgagca gtgacaaagt ggggaaaacc
      781 ggattttccg agaacgtgct cgcgaaccgg attgcgttcg ggaaaatata tcagagtgag
      841 ttacgtcagc gcgttgaggc gctgggttat gaaaccgaag tggtcggtaa gcacggtatg
      901 tgggagatgc cgggcgtacc ggtggaggcc ttttccagtc gttcacaggc tatcagggag
      961 gccgtcggtg aagacgcctc gctgaaatcc cgggatgtgg cggcactgga tacgcgaaaa
     1021 tccaaacagc acgtcgatcc ggaaatcaga atggctgaat ggatgcagac gctgaaggaa
     1081 accgggttcg acatccgggc atatcgtgac gcggcggatc agcgtgcgga gatccgcacg
     1141 caggcgcccg ggcctgcttc acaggacggg ccggatgtgc agcaggcggt gacacaggcg
     1201 attgccggat taagtgaacg aaaagtgcag ttcacgtata cggacgtgct ggccaggacg
     1261 gtcggcatac tgccgccgga aaatggtgtg attgaacggg cacgcgccgg tatcgatgag
     1321 gccatcagcc gtgagcagct tatccccctc gaccgtgaga aggggctgtt cacatcagga
     1381 attcatgtgc tcgatgagct gtccgtccgg gcactcagtc gtgacatcat gaaacagaac
     1441 cgggtgaccg tacatccgga gaaaagtgtc ccccggacgg ctggttacag cgatgcggtc
     1501 agtgttctgg cacaggaccg tccgtcgctg gccattgtgt ccgggcaggg cggtgcagcc
     1561 gggcagcgtg agcgggtggc tgaactggtc atgatggccc gggaacaggg gcgggaggtg
     1621 cagattatcg ctgctgaccg tcgctcgcag atgaacctga agcaggatga acggctgtcc
     1681 ggtgaactga taaccggacg tcgtcagctg ctggaaggca tggccttcac gccgggcagt
     1741 accgttatcg tcgaccaggg tgaaaaactc tccctgaaag agacgttaac cctgctggat
     1801 ggtgccgcac gtcataacgt acaggtcctg ataaccgaca gcgggcagcg aaccggtaca
     1861 ggcagtgcgc tgatggccat gaaggatgcc ggggtgaaca catatcgctg gcagggcgga
     1921 gaacagcgac cggccaccat catcagtgaa ccggaccgta atgtccgcta tgcccggctg
     1981 gccggagatt ttgcggccag cgtgaaagcc ggagaagaga gcgtggcaca ggtcagcggg
     2041 gtacgggaac aggccatact gacacaggcc attcgcagtg agctgaaaac gcagggcgtg
     2101 ctcggacacc ctgaggtgac catgactgca ctgtcaccgg tctggctgga cagccggagc
     2161 cgttatctgc gggatatgta ccgaccgggg atggtgatgg agcagtggaa cccggagaca
     2221 cgcagtcatg accgctatgt tatcgaccgg gtgacggcgc agagtcacag cctgaccctg
     2281 cgggatgcgc agggcgaaac gcaggtggtg cgtatttcct ccctggacag cagctggtcg
     2341 ctgttccggc cggaaaaaat gccggtggca gacggcgagc gactgagggt gacagggaaa
     2401 attcccggac tccgcgtctc cggcggtgac cgcctgcagg tggcatccgt cagtgaagat
     2461 gcgatgacgg ttgttgtgcc ggggcgggct gaaccggcca ccctgcctgt ggctgattca
     2521 ccgttcacgg cactgaagct ggagaacggc tgggtggaaa cgcccgggca ttccgtcagt
     2581 gacagtgcga cggtgtttgc ctccgtcaca cagatggcaa tggacaacgc caccctgaac
     2641 ggtctggccc gcagcggtcg tgatgtccgg ctgtattcct cactggatga aacccgtact
     2701 gcggaaaaac tcgcccgcca tccgtccttt acggtggttt ctgagcagat aaaggcgcgt
     2761 gccggtgaga cattgctgga aaccgctatc agtctgcaga aagccgggct tcacacgccg
     2821 gcacagcagg ccattcatct ggcccttcct gtgctggaaa gtaaaaacct ggccttcagc
     2881 atggtggacc tgctgacaga ggcgaagtcg tttgctgcag aaggaaccgg ttttactgaa
     2941 ctgggagggg aaatcaatgc gcagataaaa cgcggtgact tactgtatgt ggatgtggca
     3001 aaaggctatg gcacaggcct gctggtttcc cgtgcgtcgt atgaggcaga aaagagcatt
     3061 cttcgccata ttctcgaagg caaggaggcg gtcacgccgc tgatggagag agtacccggc
     3121 gaactcatgg agacgttaac gtcgggacag cgtgccgcca cccgtatgat actggaaacg
     3181 tccgaccgtt tcacggtggt acaggggtat gccggtgtgg gtaagaccac tcagttccgg
     3241 gcggtgatgt cagccgtgaa catgctgccg gagagtgagc gtccccgggt tgtggggctg
     3301 gggcccacgc accgtgcggt cggtgagatg cgcagcgccg gcgtggatgc acagacactg
     3361 gcgtcctttc tgcatgacac gcagctgcag cagcgcagcg gagaaacgcc ggatttcagc
     3421 aacacgctgt ttctgctcga tgagagctca atggtgggca ataccgacat ggcacgggca
     3481 tacgccctga ttgcggccgg tggcggtcgt gctgtggcca gcggtgacac ggaccagctg
     3541 caggccatcg cgcccggtca gcctttccgt ctccagcaga cgcgcagtgc tgccgatgtg
     3601 gccatcatga aggagattgt gcgtcagacg ccggaactgc gggaggcggt atacagcctg
     3661 attaaccggg atgtggaaag ggcactgtcc gggcttgaga gtgtgaaacc gtctcaggtg
     3721 ccacgtctgg agggcgcatg ggcaccggag cactccgtga cggagttcag tcacagccag
     3781 gaagcgaaac tggcagaagc gcagcagaag gcgatgctga aaggcgaggc tttcccggat
     3841 atccccatga cactgtatga ggccattgtc cgcgactata ccggcaggac accggaagca
     3901 cgggagcaga cgctgattgt cacgcacctg aatgaggacc ggcgcgtact gaacagcatg
     3961 attcatgatg cacgggaaaa ggccggtgag ctgggcaaag agcaggtcat ggtgcctgtc
     4021 ctgaacacag cgaatatacg tgacggagaa ctgcgtcgtc tctccacctg ggagaaaaac
     4081 ccggatgccc ttgccctggt ggatagtgtg tatcaccgga ttgccggtat cagcaaggat
     4141 gacgggctga taaccctgca ggatgcggaa ggtaacacgc ggctgatttc gccccgggag
     4201 gcggtggctg aaggtgtcac actgtacacc ccggacaaaa tccgggtggg gaccggcgac
     4261 cggatgcgct tcacgaagag tgaccgggag cgcggttatg tggccaacag cgtctggacg
     4321 gtgacagcgg tttccggtga cagtgtcacg ctgtcggacg gacagcagac ccgggtgatt
     4381 cgccccggtc aggagcgggc agagcaacat attgacctgg cctatgccat cactgcccac
     4441 ggtgcgcagg gggcgagtga aacctttgcc atcgcgcttg aaggcacgga aggtaaccgg
     4501 aaacagatgg ccggctttga gtcagcctac gtggccctgt cgcgtatgaa gcagcatgtg
     4561 caggtgtaca ccgataaccg tcagggctgg acggatgcca ttaacaatgc cgtacagaaa
     4621 ggaactgccc acgatgtgct ggagccgaaa ccggaccggg aggtcatgaa tgcgcagcgg
     4681 ctgttcagta cggcacggga actgcgggac gtggcggcag gccgtgccgt tctccgtcag
     4741 gcggggctgg ccgggggaga cagtccggca cggtttattg caccggggcg caaatatccg
     4801 cagccgtatg tggcactgcc tgcttttgac cgtaacggca agtccgccgg tatctggctg
     4861 aacccgctga ccacggatga cggaaacggg ctgcggggat tcagcggtga aggacgggtg
     4921 aaaggcagcg gggatgcgca gtttgtggcc ctgcagggca gccgtaacgg cgagagcctg
     4981 ctggctgata atatgcagga tggtgtccgg attgcccgtg ataaccccga cagcggtgtg
     5041 gtggtgagaa tcgccggtga aggtcgtccg tggaatcccg gtgccatcac cggtggtcgc
     5101 gtgtgggggg atatcccgga caacagcgtt cagccgggag ccggaaatgg cgaaccggtc
     5161 acggcagagg tactggcaca gcggcaggct gaagaggcca tccgccgtga aacggaacgt
     5221 cgcgcagatg aaattgtccg taaaatggca gagaacaaac ctgacctgcc ggacggcaaa
     5281 acagagcagg ctgtcaggga gattgccgga caggagcgtg accggactgc cacttctgaa
     5341 cgggaagccg cgctgccgga gagtgtgctg cgtgaatcac aacgggagcg ggaagcggtc
     5401 cgggaggttg cccgggaaaa tctgctgcag gagcgtctgc agcagatgga gcgggatatg
     5461 gttcgtgacc tgcagaaaga gaaaaccctg ggcggagact gatacaggaa gataaacgct
     5521 gatgacaacg gataacacga acacgacacg taacgattca ctggctgcac ggaccgatac
     5581 ctggttgcag tcatttctgg tctggtcacc cggacagcgg gatatcatca aaacggtggc
     5641 actggtgctg atggtactgg accacatcaa tctgatattc cagctgaagc aggaatggat
     5701 gtttctggcc gggcgaggag cctttccgct gtttgccctg gtgtgggggc tgaatctgtc
     5761 ccgtcatgcg catatccggc aaccggccat taaccggctg tggggatggg ggattattgc
     5821 ccagttcgca tattatctgg caggctttcc ctggtatgag gggaatatcc tgtttgcctt
     5881 tgcagtggcg gcacaggtgc tgacgtggtg tgaaacgcgc agtgggtggc gtacagcagc
     5941 ggccattctt ctgatggccc tgtgggggcc tttgtccggc accagttacg gcattgccgg
     6001 gctgctgatg ctggcagtca gccaccggct gtaccgggcg gaagacagag cggaacgtct
     6061 ggcgctggtc gcctgtctgc tggctgttat tccggcgctt aaccttgcca ccagtgatgc
     6121 agcggcggtg gctggtcttg tgatgacggt gctgaccgtt ggtctggtgt cgtgtgcagg
     6181 aaaatcatta ccccgtttct ggcccgggga ttttttcccg gtattctacg cctgtcatct
     6241 ggctgtgctg ggcgttctgg cgctgtgacg ggtgtggtat ctttgaccgt aacaggatga
     6301 ttcgtcagag gcagaacaca gcatgacaga gcagaagcga ccggtactga cactgaagcg
     6361 aaaaacggaa ggggagacgc ctgtccgcag gcggaaaacc atcatcaatg tcaccacgcc
     6421 accaaagtgg aaggtgaaaa agcagaagct ggccgagaaa gcagcccggg aagcggagct
     6481 ggcggcaaaa aaagcgcagg ccagacaggc gctgtccatt tatctgaacc tgccctcgct
     6541 ggatgaggcc gtgaacaccc tgaaaccctg gtggccggga ttatttgacg gtgaaacgcc
     6601 ccggcttctg gcctgcggta tccgggacgt gttactggaa gacgtggcgc agcggaatat
     6661 cccgctctcg cataaaaaac tgcgcagggc gctgaaggcc atcacccgtt cagaaagcta
     6721 tctgtgtgcc atgaaagccg gtgcctgccg gtatgatacg gaagggtatg tgacggagca
     6781 tatttctcag gaggaggaag cgtatgcggc agcgcgtctg gataaaatcc gccgccagaa
     6841 caggataaag gcagagcttc aggccgtgct tgatgagaaa taaaaaaaag cctccatcag
     6901 agaggaggca gggaaataag gcttaaaagg aaataacttc cgtacccgaa aggaagccaa
     6961 ttcagtagat tatttaacac cataatactg acgggttaaa gaggagtgat aaagatgaat
     7021 ggattcagaa acagttcacg gaacggtcag gtctggcgct ggcagcgtgc cggaagccgg
     7081 gccgttattc tggaggtcag tggacgctgg atggaagcgg cagaagcatg gcgacgggct
     7141 gccggtgttg cgccccggac agactggcag cagtttgccc gaaaaagggc tgaacactgc
     7201 caccggcgca gccggggaag ggtgtaacga aaaaagcagc cttcattact ccagggattc
     7261 agaaggctga ataatgcatg agatttgctt tttttaatat ccggtcgggc agtatgaccg
     7321 gacagttctg agtagcacag agttttttcc gtattaagtc gtgattttat attttgtgat
     7381 taatttcaca aaataaggtg ttattcagtg tgtgctgcga tattcaggat tacctgaaat
     7441 acaggtgcca ttt
//
LOCUS       DAZPAC010000204         7429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZPAC010000204 DAZPAC010000000
VERSION     DAZPAC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.205"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..99)
                     /locus_tag="ACHUPM_003565"
     CDS             complement(<1..99)
                     /locus_tag="ACHUPM_003565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HFR0871245.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAEE"
     gene            complement(124..1224)
                     /locus_tag="ACHUPM_003566"
     CDS             complement(124..1224)
                     /locus_tag="ACHUPM_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR0871246.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(1612..2358)
                     /gene="cutC"
                     /locus_tag="ACHUPM_003567"
     CDS             complement(1612..2358)
                     /gene="cutC"
                     /locus_tag="ACHUPM_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR0871247.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(2372..2938)
                     /locus_tag="ACHUPM_003568"
     CDS             complement(2372..2938)
                     /locus_tag="ACHUPM_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0871248.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            3154..4890
                     /gene="argS"
                     /locus_tag="ACHUPM_003569"
     CDS             3154..4890
                     /gene="argS"
                     /locus_tag="ACHUPM_003569"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR0871249.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            complement(4932..4976)
                     /gene="yecV"
                     /locus_tag="ACHUPM_003570"
     CDS             complement(4932..4976)
                     /gene="yecV"
                     /locus_tag="ACHUPM_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFR0871250.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            5067..5429
                     /locus_tag="ACHUPM_003571"
                     /pseudo
     CDS             5067..5429
                     /locus_tag="ACHUPM_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(5433..6503)
                     /locus_tag="ACHUPM_003572"
                     /pseudo
     CDS             complement(5433..6503)
                     /locus_tag="ACHUPM_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(6496..>7429)
                     /gene="flhB"
                     /locus_tag="ACHUPM_003573"
     CDS             complement(6496..>7429)
                     /gene="flhB"
                     /locus_tag="ACHUPM_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFR0871251.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1920 a         1947 c         1776 g         1786 t
ORIGIN      
        1 ctcttctgcc catgcgggga gcggtgctgc cgatgtcacc acctgcgctc cagcggcaat
       61 accgctgtgt ttaataaatt cacgtcttgt taatgtcata acttcctccc tgatcaacga
      121 ggatcactgt ttctcggtaa tatctttggc gttgtactgg aaataccgcg ttaaaatgtc
      181 cagttcgttt tcgctcatgc tggttcgtgc ccccattcct ttggcaatgg acggccacgc
      241 attgacggtg taatggtcgg cggcaatagg ggcatgacaa ccagcgcaat aggtatcgtc
      301 aagtttttca gcgtattgcc atagcggttt acggtccgct aatccgggat cggtaagcgc
      361 accctgtaaa gacgcctgac gccattgatt gccgtattcg tcagcctgcc attccccgtt
      421 taccgtgagc gccttgatac cttcttcact taatgtggct agcgccagcc gttgacctgc
      481 cgccaggtag agcgtgtttt cactgccctg catttgataa ccctgcaaca gaacgatcgg
      541 ctgtttgcca ctggcatcaa cgacggtgag atcggtacca ggattcacgg tagccagctc
      601 gcctatgcga gaagttttga aaggataaat atgtgcgcca ttagtcactg aagtagcggc
      661 ctgactttcc agctcatgcg ccgcgttgtc atccattttt atttctggcg gaaaatgggc
      721 aatgccttta tgacaatcga tacaggtttc gccgcccttt tgtgctttgt tatgcatttt
      781 ctgcgcagat tcactttgcg aggcaatatc catggcatca aaagaatggc aactacggca
      841 cgttgcagag tcagttgctt ttaattcttt ccatactgtt tcggccattt cctggcgatg
      901 agtttcgaac ttatcgtcac tgtctatttt gccgctaaca aattcatgat aaatatcttt
      961 agatgctttt aatttagcaa ataaataatc catccctgac tttggaatat ggcaatctgc
     1021 acattccgca cgtatccctt tctggttcga aaagtggaca gttccctgat attcctcaaa
     1081 agatttactc atcgagtggc aggaaaggca aaatgctgta tccgacgttt tatgtaaggc
     1141 tttttgcgcc agcaataacc cgccgccacc aaccacaacc gctatcagca aaaacaataa
     1201 cccaatgcgt tttttccctc gcatagattc ctctaaataa attccagaac accggaatca
     1261 ttgtataatt aaatacacaa cgaattaacc gcttgcataa ctcggcacaa cactgcctga
     1321 aataatcgat aaagaatatg atttattaca atgtaatcat taattgctaa ggaataaccc
     1381 agttgctatt tagaatatat atttatcagt tttagcaatt taaatcccat aatcaatgtg
     1441 aatataaaaa atattattag cacaccgcta tgttcactgt cacccgctca acagatcacg
     1501 gttatcgttc gttttttata ctgctcaggg ataaaaaaag gcccctgttg aaattgcagg
     1561 ggcctggtac gagcaagcat catattgggc gacatgatgc aacggtaaaa atcatttggc
     1621 ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc gccccgtcta cgatataacg
     1681 cgaatactcg tccgcgtgtt catctgatga catggacaat ccttgattac gataacgcat
     1741 cggtgaggct tgccacgctc ccgcggagct atggacttcc agcactccgg catcgaggaa
     1801 gtggtgcagg ttttctgcac ggactcctgc tccggccata atgattggag catcacgatg
     1861 ggcaataagt tccataattt ttgataaacc ttgcagtgcg tctgattttt gccctgatgt
     1921 cagtacccgg gcaatgccca attccgcaag attattgagt gtatataaag ggttagcgca
     1981 catatcgaag gcgcgatgaa aagtcactgc cagcggaccg gcagcagcca ttattttttc
     2041 cattcgtggc atatcgacat tcccgtcaac atcgagaacg cccgtcacca gtccaggaaa
     2101 acctaattcg cggaccgtgc gcacatcctc aagaatggcg gcaaactcac cgtcgctgta
     2161 gcaaaaatca ccaccgcgtg ggcgaattat cggatgcaca gggatcgtca cccgctggcg
     2221 cacggatttc agtaccccca acgacggcgt taagcccccc tcttttgggg ctgcgcataa
     2281 ttcaactctg tctgcgccgt tttgctgcgc cgttagtgca cattccatgc tgtaacagca
     2341 aatttccagt aatgccattt ttgctcctta attacgctga ctgctcgctg gcaacgatct
     2401 cttcaatgga ccacggatga aacttaatag ttgttttgcc atcggtgacc gccagcgtcg
     2461 gattcggtaa tcgctcatgc tcaccttttg gtgaactggt tttaactgag atccccggta
     2521 ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc attcagcgtt tctggatcac
     2581 ccagcagaac gatttcaata tgttcccaac cttcgtgtgg gtaacgtttt tccccgggcc
     2641 acggtagctc cacaatagaa aactgccagt gcgcaacctg taccggttca tgcaatttaa
     2701 acagacaaat cggtctgcca ttgatcatat tttctgacaa aagctcgcca cactgttcaa
     2761 acccgcgacg ccagcgttca gcagtggcgt tttgatggca acgcaaagaa atgtgatcgg
     2821 cagtcagcgg agtgatattc aaccccagac ggagggaaag ttcatctaat gcgtgggtaa
     2881 atcgcggtaa atccgatgca atatcctgca gctcgtcgat agattgccag ttcgccataa
     2941 tcactcttcg tctttcagta aaagcgttaa tttaccctgt tgccctgcgc caaccaaccg
     3001 ctgatttcac gccgcttctg atgcaatagt gaaaacggca atacgccacg cgcacgttgc
     3061 tgacgaaaac agccatttgc agtatactcc cgccctaatt tctttaactg gtgcgggcaa
     3121 tttttgctcg cttcatcaat gtaaggtatt ccggtgaata ttcaggctct tctctcagaa
     3181 aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg attgcgaacc gcaggttcgt
     3241 cattcagcaa aagttcagtt cggcgactat caggctaacg gcatgatggc agttgctaaa
     3301 aaactgggta tgacaccgcg acaattagca gagcaggtgc tgactcatct ggatcttaac
     3361 ggtatcgcca gcaaagttga gatcgccggt ccaggcttta tcaacatttt ccttgatccg
     3421 gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg atcgtctcgg tgtttctacg
     3481 ccagaaaaac agaccattgt ggttgactac tctgcgccaa acgtggcgaa agagatgcat
     3541 gtcggtcacc tgcgctctac cattattggt gacgcagcag tgcgtactct ggagttcctc
     3601 ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg gcactcagtt cggtatgctg
     3661 attgcatggc tggaaaagca gcagcaggaa aacgccggtg atgaaatgga gctggctgac
     3721 cttgaaggtt tctaccgcga tgcgaaaaag cattacgacg aagatgaaga gtttgccgag
     3781 cgtgcacgta actacgtggt aaaactgcaa agcggtgacg aatatttccg cgagatgtgg
     3841 cgcaaactgg tcgacatcac catgacgcag aaccagatca cctacgatcg tctcaacgtg
     3901 acgctgaccc gtgatgacgt gatgggcgaa agcctctaca acccaatgct gccaggaatt
     3961 gtggcggatc tcaaagccaa aggtctggca gtagaaagcg aaggggcgac cgtcgtattc
     4021 cttgatgagt ttaaaaacaa ggaaggcgaa ccgatgggcg tgatcattca gaagaaagat
     4081 ggcggctatc tctacaccac cactgatatc gcctgtgcga aatatcgtta tgaaacactg
     4141 catgccgatc gcgtgctgta ttacatcgac tcccgtcagc atcaacacct gatgcaggca
     4201 tgggcgatcg tccgtaaagc aggctatgta ccggaatccg taccgctgga acaccacatg
     4261 ttcggcatga tgctgggtaa agacggcaaa ccgttcaaaa cccgcgcggg tggtacagtg
     4321 aaactggccg atctgctgga tgaagccctg gaacgtgcac gccgtctggt ggcagaaaag
     4381 aacccggata tgccagctga cgagctggaa aaactggcta acgcggttgg tattggtgcg
     4441 gtgaaatatg cggatctctc caaaaaccgc accacggact acatcttcga ctgggacaac
     4501 atgctggcgt ttgagggtaa taccgcgcca tacatgcagt atgcttacac gcgtgtattg
     4561 tccgtgttcc gtaaggcaga aattaacgaa gagcaactgg ctgcagctcc ggtaatcatc
     4621 cgtgaagatc gtgaagcgca actggcagct cgcctgctgc agtttgaaga aaccctcacc
     4681 gtggttgccc gtgaaggcac gccgcatgtg atgtgtgctt acctgtacga tctggctggt
     4741 ctgttctctg gcttctacga gcactgcccg atcctcagcg cagaaaacga agaagtgcgt
     4801 aacagccgtc tgaaactggc acaactgaca gcgaagacgc taaagctggg tctggatacg
     4861 ctgggtattg agacagtaga gcgtatgtaa tcgatttttc gtgagagtaa agcctgatca
     4921 gggttacctg atcaggcttt tttattgcca tctaaatgta ttctgaaaat ggacatgcca
     4981 ttgttttctg actgttggat aagaggccag aagcgtaata tccggcccca gggaaacgat
     5041 aacggttgaa tttaaggaat accgcagtgt ttaaatttct tgtattaaca ttaggcatta
     5101 tctcttgcca ggcttacgca gaagatacgg ttatagaaaa cgaccatgac atttcagcca
     5161 tcaaagattg ttggcaaaaa aattcagatg atgatactga cattaacgtg atcaaatcat
     5221 gcctgcgaca agaatacaat ctcgtcgatg cgcaattaaa taaagcctat ggtgaagctt
     5281 atcgttatat agaacaagtg ccacgcacag gtgtaaaaaa acctgatacc gaacaactta
     5341 acttgcttaa aaaatcacag cgagcctggc tggattttag ggacaaagaa tgtgaattaa
     5401 tcctttcaaa tgaggacgtt caggatttaa tgtacatcgt tccacgtcgc ttcgacaacg
     5461 gtattattct ctgccatttt taccggtttg ggttcggcag gcgctttttg ttcgcgtccg
     5521 cgtatccacc aggccagccc gagcaatccg gcagtgaaca gcaaaaatac caggttcggc
     5581 attccaggca ccaggccgag taaaccgagc acggcggcgc ttaacaacat aacgcttggg
     5641 ttactgaaaa gctgattcac catctgctcg ccaacatcct gatcggtgct gacacgcgta
     5701 acgatgaccc ccgcggcggt aaaaatcacc agcgccggaa tttgtgccac cagaccatcg
     5761 ccaatggtca atagcgtata actttccgcc gcgtgtccca tgctcatgcc atgttgcagc
     5821 acgccgacca gcaacccgcc gacaacgtta atgaccatga tgaggatccc ggcgatggca
     5881 tcgccgcgaa caaacttact tgccccgtcc attgagccgt aaaaatcggc ttcctgagtc
     5941 acttcggagc ggcgtttttt cgcctcatct tcaccaatca atccagcgtt aaggtcggcg
     6001 tcaatcgcca tctgcttacc cggcatacca tcgagaacaa agcgcgcacc cacttctgcg
     6061 atacgcccgg cacctttggt aatgaccata aagttgatga tcacgagaat gacaaacacc
     6121 acgataccga tagcgaaatt gccaccaacg aggaagtgac cgaacgcttc gaccaccttc
     6181 cctgccgccg ccgcgccggt atgcccttcc atcaaaatga tacgggttga agccacgtta
     6241 agtgccagac gcaacaacgt agtaaacaac agaatggtcg gaaacgcagc aaactcaagc
     6301 gtgcgctggg taaacatcgc caccagcaac accatgatcg acaaggcaat attgaaggta
     6361 aacaacaggt cgagtatgaa tgcgggcagt ggcagcacca tcatcgacaa gatcaacagg
     6421 atcaaaatcg gtccggcaag gatctgccat tgtgtcgatt tcaggtttgc gggcaggcgc
     6481 agcatcgcgg ccagattact catgggtcgg tttctcgtta ataaaatcca gggcttccgg
     6541 caccggaaga tgagtaggtt gtacagggcg ctgtccacca gccagacgcc agcgtttcag
     6601 ttgccagacc caggccagca cttccgccac cgcggcgtac agttgacccg ggatttgttg
     6661 accaatctcc gcatgtcgat acagcgctcg cgccagcggc ggcgcttcaa gcgtcgggac
     6721 gttattttca gcgccaattt cacgaatgcg cagcgcgacc agccctgcac ctttagcgac
     6781 cactttcggt gcgctcattt tgttttcgtc atactgcaac gctaccgaat agtgggtcgg
     6841 gttattgaca atgacatcag ctttcggcac atcggccatc atccgacgcc gtgcagcagc
     6901 tcgctgcatc tgacggatcc gccctttaac atgagggtca ccttcgcttt gtttgaactc
     6961 atcacgaata tcctgccgcg acatacgcag ctttttcagg tggctgaaga tttggaaaaa
     7021 gacgtcaaat cccaccattg gaatgacacc aagcaccacc agcagtgcgc atagcccaac
     7081 cagatccatc gcattaccca tggcggtagt cggagactcg gccattaagc gcatcatctg
     7141 cggccagtga tgccagagat aaaaccccgt cacgctgcca accaggatgg ttttcaaaat
     7201 tgccttaagc aactccgcgc cagtctgtgc cgagaacatc cgtttaatgc ccggtagcgg
     7261 gttgagtttg gaaaacttcg gctgcaagga tttgccgcta aataccagcc cccccagcat
     7321 gaccggagaa atgagcgcca ccagcaccac gccgctaatc agcggcagca gcgccagcat
     7381 ggcttctctg atcagcagaa taatctgccc gaggatcaga ttcgggtca
//
LOCUS       DAZPAC010000205         7424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZPAC010000205 DAZPAC010000000
VERSION     DAZPAC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.206"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(83..1591)
                     /gene="putP"
                     /locus_tag="ACHUPM_003574"
     CDS             complement(83..1591)
                     /gene="putP"
                     /locus_tag="ACHUPM_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFR0871252.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            2013..5975
                     /gene="putA"
                     /locus_tag="ACHUPM_003575"
     CDS             2013..5975
                     /gene="putA"
                     /locus_tag="ACHUPM_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0871253.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(6015..6653)
                     /gene="rutR"
                     /locus_tag="ACHUPM_003576"
     CDS             complement(6015..6653)
                     /gene="rutR"
                     /locus_tag="ACHUPM_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR0871254.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            6941..>7424
                     /locus_tag="ACHUPM_003577"
     CDS             6941..>7424
                     /locus_tag="ACHUPM_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR0871255.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         1774 a         2057 c         1943 g         1650 t
ORIGIN      
        1 ccattcaatt tatatggcga ggcacattaa gggagcgcaa cccgctgctg aagccaaaca
       61 aaaccgccgc aggctaagtc ccttagtttt cctgcaaccg tgacggcgga gccgaatgat
      121 agtgcgcatc ggcctcggca aagcgttttt gcatcgccgc tgacggcgct ttacccagca
      181 aactaaacac tacaatcccg atactgccga agatgaagcc cggaataatt tcgtacagtc
      241 ccagccagcc gaactgcttc cagacgataa ctgtcagcgc accgatgatc atccccgcca
      301 gcgcaccgtt gcgcgtcatg cgtgaccaca tcaccgagaa cagcaccacc ggaccaaacg
      361 ccgcgccaaa gcctgcccac gcgtagctca ctaagcccag cacgcggttt tccgggtttg
      421 ccgccagcgc aatcgccacc agcgccacca ccagcaccat cacacgtcct acccacacca
      481 gctctttctg gctggcctgt ttacgcagaa acgctttgta taaatcttcg gtaatcgcac
      541 tggagcacac cagcagctgg caacttaagg ttgacattac cgccgccaga atcgccgaca
      601 gcagaatccc ggcaatccac gggttaaaca gaatttgcgc cagttcgata aacacgcgtt
      661 cagcgttctg gtttaccgca cctgctaccg ccggatgctc gttaaagtaa gcgataccaa
      721 agaagccaac agccaccgcc cctgccaggc aaaggatcat ccaggtcata ctgatacgac
      781 gcgcatggac aatgctgtgg tgagaatctg ccgccataaa acgcgccagg atgtgcggct
      841 gcccgaagta acccagcccc caacccatca atgagataat ggcaacaaag ttcagccctt
      901 tgagcatatc cacgttttcg atgctctttt gtttgatcac ttccagcgag tcaccaaagc
      961 caccgacact gataatgacg ataaccggcg tcaggatcag ggcaaaaatc atcaggctgg
     1021 cctgtacagt gtcagtccag ctcaccgcga ggaaaccgcc aataaaggtg taaaggatcg
     1081 tcgacgcagc tcccgccaac agagccgttt cgtaactcat gccaaaggta ctttcaaaca
     1141 gacgcgcgcc tgccacaatg cccgaagcgc aataaatggt gaagaacagc aaaataacca
     1201 gcgcggagat aatgcgcaaa atgcggcttt tatcttcaaa acgcccggtg aaataatccg
     1261 gcaatgttaa ggcgttatta ttgtattcgg tatgcacacg caaccgcccg gccaccagct
     1321 tccagttgat ccacgcgcct aatgtcaggc caatggcgat ccagctttcg gaaatcccgg
     1381 aaagaaaaac cgcgccaggc aaccccatta acagccagcc gctcatatcc gacgcacccg
     1441 ccgataatgc cgtcacgaat ggcccaagac tacgaccgcc cagaatatag tcgtcaaagt
     1501 ttttcgttga tcgccaggcg ataaacccaa tcaatatcat gccaaagata tagacacaaa
     1561 atgtcaccaa catcggtgtg ctaatagcca tctaaagtct ccaaaaaatt attatcggca
     1621 atgtcgaaac ttgccgttat atctgccacc ggaacggggt aacagagttt atgttttacc
     1681 agggcgaccg tatcctgccg gaagcgctgg ttattcacaa tcgatttaac acaccattta
     1741 cattaaattt tagtgctcag cgacactatt tttcatcagg ttgcactctc tcacattttt
     1801 tgcggttgca cctttcaaaa aggttaactg ccgcagagaa aaagtctgag ttatttttta
     1861 atcactgtca tatcgatttc ttttattaac atttcattca tttttaagct tgctacgcaa
     1921 gtcacattta acatggttgc acaaagttgc aacatcatgg atatttcacg ataacgttaa
     1981 gttgcacctt tcagaacaac aggagtaatg gcatgggaac caccaccatg ggggttaagc
     2041 tggacgacgc gacgcgtgag cgtattaagt ctgccgcgac acgtatcgat cgcacaccac
     2101 actggttaat taagcaggcg attttttctt atctcgaaca actggaaaac agcgatactc
     2161 tgccggagct acctgcgctg ctttctggcg cggccaatga gagcgatgaa gcaccgactc
     2221 cggcagagga accacaccag ccattcctcg actttgccga gcaaatattg ccccagtcgg
     2281 tttcccgcgc cgcgatcacc gcggcctatc gccgcccgga aaccgaagcg gtttctatgc
     2341 tgctggaaca agcccgcctg ccgcagccag ttgctgaaca ggcgcacaaa ctggcgtatc
     2401 aactggccga taaactgcgt aatcaaaaaa atgccagtgg tcgtgcagga atggtccagg
     2461 ggttattgca ggagttttcg ctgtcatcgc aggaaggcgt ggcgctgatg tgtctggcag
     2521 aagcgttgtt gcgtattccc gacaaagcca cccgcgacgc gttaattcgc gacaaaatca
     2581 gcaacggtaa ctggcagtca cacattggtc gtagcccgtc actgtttgtt aatgccgcta
     2641 cctgggggct gctgtttact ggcaaactgg tttccaccca taacgaagcc agcctctccc
     2701 gctcgctgaa ccgcattatc ggtaaaagcg gtgaaccgct gatccgcaaa ggtgtggata
     2761 tggcgatgcg cctgatgggc gagcagttcg tcacgggtga aaccatcgcg gaagcgttag
     2821 ccaatgcccg caagctggaa gagaaaggtt tccgttactc ttacgatatg ctgggcgaag
     2881 ctgcactgac tgccgcagac gcacaggcgt atatggtttc ctatcagcag gcgattcacg
     2941 ccattggtaa agcgtctaac ggtcgtggca tctatgaagg tccgggcatt tcaatcaaac
     3001 tgtcggcgct gcatccgcgc tacagccgcg cccagtatga ccgggtaatg gaagagcttt
     3061 acccgcgtct gaaatcactc accctgctgg cgcgtcagta tgatattggt attaacatcg
     3121 atgccgaaga ggccgatcgt ctggagatct ccctcgatct gctggaaaat ctctgtttcg
     3181 agccggaact ggcaggctgg aacggcatcg gttttgttat tcaggcttat caaaaacgct
     3241 gcccgttggt gatcgattac ctggttgatc tcgccacccg cagccgtcgc cgtctgatga
     3301 ttcgcctggt gaaaggcgcg tactgggata gtgaaatcaa gcgtgcgcag atggacggcc
     3361 ttgaaggcta tccggtttat acccgcaagg tgtataccga cgtttcttat ctcgcctgtg
     3421 cgaaaaagct gctggcggtg ccgaatctaa tctacccgca gttcgcgacg cacaacgccc
     3481 atacgctggc agcgatttat caactggcag gtcagaacta ctatccgggt cagtacgagt
     3541 tccagtgcct gcatggtatg ggcgagccac tgtacgagca ggtcaccggg aaagttgccg
     3601 acggtaaact taaccgaccg tgtcgtattt atgccccggt tggcacacat gaaacgctgc
     3661 tggcgtatct ggtgcgtcgc ctgctggaaa acggtgctaa cacctcgttt gttaaccgta
     3721 ttgccgacac ctctttgcca ctggatgaac tggtcgccga tccggtcact gctgtagaaa
     3781 aactggcgca acaggaaggg caaactggat taccgcatcc gaaaattccc ctgccgcgcg
     3841 atctttacgg tcacgggcgc gacaactcgg cagggctgga tctcgctaac gaacaccgtc
     3901 tggcctcgct ctcctctgcc ctgctcaata gtgcactgca aaaatggcag gccttgccaa
     3961 tgctggaaca accggtagcg gcaggtgaga tgtcgcccgt tattaaccct gcggaaccga
     4021 aagatattgt gggctttgtg cgtgaagcca cgccacgtga agtagaacag gcgctggaaa
     4081 gtgcggttaa taacgcgcca atctggtttg ccacgcctcc ggctgaacgc gcggcgattt
     4141 tgcatcgcgc tgccgtgcta atggaaagcc agatgcagca actgattggt attctggtgc
     4201 gtgaagccgg aaaaaccttc agtaacgcca ttgccgaagt gcgcgaagcg gtcgattttc
     4261 tccactacta cgccggacag gtgcgggatg atttcgctaa cgaaacccac cgtccattag
     4321 ggcctgtggt gtgtatcagt ccgtggaact tcccgctggc tattttcacc gggcagatcg
     4381 ctgccgcact ggcggcaggt aacagcgtgc tggcaaaacc ggcagaacaa acgccgctga
     4441 ttgccgcgca agggatcgcc attttgctgg aagcgggtgt accccctggc gtggtgcaat
     4501 tgctgccagg tcagggggaa accgtgggcg cgcaactgac gggtgatgat cgcgtgcgcg
     4561 gggtgatgtt taccggttca accgaagtcg ctacgttact gcagcgcaat atcgccagcc
     4621 gcctggacgc tcagggtcgc cctattccgc tcatcgctga aaccggcggc atgaacgcga
     4681 tgattgtcga ttcttcagca ctgaccgaac aagtcgtcgt ggatgtgctg gcctcggcgt
     4741 tcgacagtgc gggtcagcgt tgttcggcac tgcgcgtgct gtgcctgcaa gatgagattg
     4801 ccgaccacac gttgaaaatg ctgcgcggtg caatggccga atgccggatg ggtaatccgg
     4861 gtcgcctgac caccgatatc ggtccggtga ttgatagcga agcgaaagcc aatattgagc
     4921 gccatattca gaccatgcgt agcaaaggcc gtccggtgtt ccaggcggtg cgggaaaaca
     4981 gcgaagatgc ccgtgaatgg caaagcggca cctttgtcgc cccgacgctg atcgaactgg
     5041 atgactttgc cgaattgcac aaagaggtct ttggtccggt gctgcatgtg gtgcgttaca
     5101 accgtaacca gctaccagag ctgatcgagc agattaacgc ttccggctat ggtctgacgc
     5161 ttggtgtcca tacgcgtatt gatgaaacca tcgcccaggt cactggctcg gcccatgttg
     5221 gtaacctgta tgttaaccgt aatatggtgg gcgcagtggt tggtgtgcag ccgttcggcg
     5281 gcgaagggtt gtccggtacc gggccgaaag caggcggtcc gctctatctc taccgtctgc
     5341 tggcgaatcg cccggaaagt gcgctggcag tgacgctcgc gcgtcaggat gcagagtatc
     5401 cggtcgatgc acagttgaaa gccgcattga ctcagccgct aaatgcactg cgggaatggg
     5461 cagcaaatcg tccagaattg caggcgttat gtacgcaata tggcgagctg gcgcaggcag
     5521 gaacacaacg attgctgccg gggccgacgg gtgaacgcaa cacctggacg ctgctgccgc
     5581 gtgagcgcgt gttgtgtatt gccgatgatg agcaggatgc gctgactcag ctcgccgccg
     5641 tgctggcggt gggcagccag gtactgtggc cggatgacgc gctgcatcgt cagctagtaa
     5701 aggcattgcc atcggcagtc agcgaacgta ttcaactggc gaaagcggaa aatataaccg
     5761 ctcaaccctt tgatgcggtg atcttccacg gtgattcgga tcagcttcgc gcattgtgtg
     5821 aagccgttgc tgcgcgggat ggcgcaattg tttcggtgca gggttttgcc cgtggcgaaa
     5881 gcaatatcct tctggaacgg ctgtatatcg aacgttcgct gagtgtgaat accgctgccg
     5941 ctggcggtaa cgccagttta atgacgatcg gttaaacagg tagccggagg atgttacaac
     6001 ctcctccggc atctttaacg tggtcgaatc ccctcaataa taatccgctg cacgttttca
     6061 accgtttgat tgaaaaatat ctcatcgcgc aacgtcgcgc ctgtcaccgc ctccacctga
     6121 ggggcgaaat cggcgtaatg ttgagtggaa gcccaaatca tgaaaatcaa atgctgcgga
     6181 tcaatcggcg cgagtttgcc gcttttgacc caaccggcaa tcagcgcaga tttctcatca
     6241 attaatgcct tcaaatcgcc cgtcagttca tccattaaca gcggcgcgcc tgccagcatc
     6301 tccatacaga acaggcgcga agcctgcgga taatcgcggg agacttccag cttcagacgg
     6361 atgtactctt ttatcgccgc cagcggagcg aaatcttcac gaaacgcttt taacggtgcc
     6421 agccagatat cgagaatctg ccgcagcacg gcaatataca gcgcctcttt tgacggaaag
     6481 taatacagca gattggtttt tgaaacaccc gccaactctg cgatctgctc cagccttgtg
     6541 ccgtgaaaac cgaattgtga aaaagtgtcc agtgctgcgc taagaatcgc ttttttcttc
     6601 gcgcttactg cgcgcgaacg tttacccgtt gttttcactg cgccttgcgt catgcgctct
     6661 cccctcttgt agattggcaa caggatagca aaacccccgc ccggcctcga ccttctgcac
     6721 tctcatcgcg cacagatgca tgttttatgt gcaactgttt tgaccgttta gtccactttt
     6781 tatcagatat tttagataac gtaatattaa catattaata acattaactt tttaaaaact
     6841 ggcatccgct ttgcaaacaa gccaatacgc ggctgaaaag gatatgcagg atgcagcgcc
     6901 ccgtctgact ttcactttac gagacgaaga gaggttgatg atgaaaattg gcgtattcgt
     6961 acctattggc aacaacggct ggctcatttc gacccacgcg ccgcagtaca tgccgacctt
     7021 tgaactgaat aaagccattg tgcagaaagc ggagcattac catttcgatt tcgccctgtc
     7081 gatgatcaaa ctgcgtggct ttggcggcaa aactgagttc tgggatcaca accttgagtc
     7141 gttcaccttg atggcgggtc tggcagccgt gacctcgcgc attcagattt acgccactgc
     7201 tgccacctta acgttacctc cggcaatcgt cgcccgtatg gccgcaacca tcgactccat
     7261 ctctggcggg cgttttggcg tcaacctcgt gactggctgg caaaagcccg tgtatgagca
     7321 gatgggtatc tggcctggcg atgactattt ctcccgtcgt tacgactatc tcaccgaata
     7381 tgttcaggtg ctgcgcgacc tgtggggcac ggggaaaagc gatt
//
LOCUS       DAZPAC010000206         7333 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZPAC010000206 DAZPAC010000000
VERSION     DAZPAC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.207"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            156..1220
                     /gene="ulaG"
                     /locus_tag="ACHUPM_003578"
     CDS             156..1220
                     /gene="ulaG"
                     /locus_tag="ACHUPM_003578"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR0871256.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1328..2083
                     /gene="ulaR"
                     /locus_tag="ACHUPM_003579"
     CDS             1328..2083
                     /gene="ulaR"
                     /locus_tag="ACHUPM_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR0871257.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2080..2829)
                     /gene="yjfP"
                     /locus_tag="ACHUPM_003580"
     CDS             complement(2080..2829)
                     /gene="yjfP"
                     /locus_tag="ACHUPM_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0871258.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3011..3340
                     /gene="bsmA"
                     /locus_tag="ACHUPM_003581"
     CDS             3011..3340
                     /gene="bsmA"
                     /locus_tag="ACHUPM_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR0871259.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3489..3764
                     /gene="yjfN"
                     /locus_tag="ACHUPM_003582"
     CDS             3489..3764
                     /gene="yjfN"
                     /locus_tag="ACHUPM_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR0871260.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3881..5506)
                     /locus_tag="ACHUPM_003583"
     CDS             complement(3881..5506)
                     /locus_tag="ACHUPM_003583"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR0871261.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5590..6753)
                     /locus_tag="ACHUPM_003584"
     CDS             complement(5590..6753)
                     /locus_tag="ACHUPM_003584"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0871262.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6756..>7333)
                     /locus_tag="ACHUPM_003585"
     CDS             complement(6756..>7333)
                     /locus_tag="ACHUPM_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR0871263.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
BASE COUNT         1915 a         1959 c         1798 g         1661 t
ORIGIN      
        1 acattccatt aaatctttcc acactcaaaa tcaacaacaa cttccctggc aaaaagtcaa
       61 aaaatggtca ttgccttcct gaaaaaatag tgtcaggatt aatcaaaacc aatcacatat
      121 tgattcgaat tgaactatca tgaaggtaaa tggcgatgag taaagtgaaa agtatcaccc
      181 gagaatcctg gatcctgagc actttcccgg agtggggtag ctggctgaat gaagaaattg
      241 aacaagagca ggtcgcccct ggcacatttg cgatgtggtg gctaggctgc accgggatct
      301 ggttgaaatc agaaggtggc accaacgttt gcgttgattt ctggtgcggc actggcaaac
      361 aaagtcacgg taacccgtta atgaaacagg gtcaccagat gcagcgcatg gctggcgtga
      421 aaaaactgca gccaaaccta cgcaccaccc cgtttgttct tgatccgttt gcgattcgcc
      481 agatcgacgc ggtactggcg actcacgatc acaacgatca tatcgacgtt aacgtcgctg
      541 ctgccgtgat gcagaactgt gctgatgacg taccattcat tggaccgaaa acctgcgttg
      601 atttgtggat tggctggggc gtaccgaaag agcgttgcat cgtggtcaaa ccgggcgatg
      661 ttgtaaaagt gaaagacatt gaaattcatg cgcttgacgc tttcgaccgt actgcactga
      721 tcaccctgcc tgccgatcaa aaagcggctg gcgtactgcc agatggcatg gacgatcgcg
      781 cagtaaacta cctgttcaaa acccctggcg gttccctata tcacagtggc gattcccact
      841 actctaacta ttatgcgaag cacggtaacg aacatcagat cgatgtggcg ttaggatcgt
      901 atggtgagaa cccgcgcggt attaccgaca aaatgaccag cgccgatatg ctgcgtatgg
      961 gtgaagccct gaatgcgaaa gtggttatcc cgttccacca cgacatctgg tcaaacttcc
     1021 aggccgatcc gcaagagatc cgcgtgctgt gggagatgaa aaaagatcgt ctgaagtatg
     1081 gcttcaagcc gtttatctgg caggttggcg gcaaatttac ctggccgctg gataaagaca
     1141 acttcgagta ccactatccg cgcggtttcg atgattgctt cactattgaa ccggatctgc
     1201 cgttcaagtc attcctgtaa tctgtcgttt ataccggata gcgtggagaa gttcgcgcta
     1261 tccggcgtag tatttcctag ctatttcaaa tatcatctaa aaaaatcaaa ttttatcgga
     1321 atagctcatg actgaagcac aaagacatca aatcctcctg gaaatgctcg cacaattggg
     1381 ctttgtgacc gttgagaaag tcgttgagcg tctgggaatt tcgcctgcca ctgcgcgacg
     1441 cgatatcaat aaacttgacg aacgcggcaa actgaaaaag gtgcgcaatg gcgcagaagc
     1501 tattacccaa cagcgcccgc gctggacgcc gatgaatctg catcaggcgc agaatcacga
     1561 tgaaaaagta cgtattgcta aagcggcctc gcagctggtt aatccgggcg aaagcgtagt
     1621 catcaactgc ggctccaccg cgtttctgct tgggcgggaa atgtgtggca agccagtgca
     1681 aatcatcact aattatctac cgctggcaaa ttacctgatc gatcaagaac atgacagcgt
     1741 gatcattatg ggtggacagt acaacaaaag tcagtccatc actttaagcc cgcagggcag
     1801 cgaaaacagt ctctatgccg ggcactggat gtttaccagc ggaaaagggc tgaccgcaga
     1861 agggttgtat aaaaccgata tgctgacagc aatggcagag cagaagatgc tgagcgtggt
     1921 agggaaactg gtggtactgg ttgatagcag taagattggc gaacgcgcgg gaatgctttt
     1981 tagccgtgcc gatcaaatcg atatgcttat caccggcaaa aatgctaacc cggaaatcct
     2041 gcaacaactg gaagcgcaag gggtcagcat tctgcgtgtt taaagatgct ggcggaaaaa
     2101 tgtcacggca gcatctaacg cctcaggcgt aatgcggtga cgcacgcctg gctgccatga
     2161 acaggttagc agtttatccc gtcccgtctc gcttaaggcc tgctgcaaac gtagtgattc
     2221 gtcggcaggc acaacgtcat cgtcgaggcc atgccacagc agtagaggtc tgtccccaag
     2281 ttgttccagg tggtttgtcg cttcccactc tgccagcggc gcgataatgt tattgaattc
     2341 attctgctgt gctgccgttt caggtatcag cggtggaaac agtgaacggg cgagcgatgt
     2401 aaaatagccc gatcccatca tgctggcggt acatttcacc gtggggtggc gagcggtaat
     2461 tcccagtgcc gtcatcgcgc ccatcgacgc gccaccgact gccagacggt catcaagcag
     2521 ccagttttct tcggctattg ccgcacgtaa agtagtgaat tcctgcatac tttgtagcaa
     2581 gatttgccag aattgattta accgccgcgc ttcgtcgcca ctaaaacggc taccgtgatc
     2641 gggcgcatcc ggcatgatca cccgcaaacc agcctgcgcc agcgcaacgg caaaatagct
     2701 atacaccagg ctggatgaag taaagccgtg ataaaaaatt acgcacggta acggggtatc
     2761 tttttgcccg acaggataag catgaagaac gggaatatct gccagctcgc gtgattctat
     2821 ttcaatcatc tgtctctcct ttttgtctgg tatgacatga agaaatcctt agcacaagtt
     2881 tctgctggcg gtattagttc ataatgttga gatgtgggtt acgctttcgt cacgttttca
     2941 ttcgaaaatc gcgtaccagg taacaattcg ggaacattcc cccaaagtta aattaataag
     3001 gcactacact atggttagca ggaaacgtaa tagcgttatt taccggtttg ccagtttatt
     3061 attggtgttg atgttaagtg cctgtagcgc actgcaaggt acgccacagc cagcaccgcc
     3121 agtaacggac catccgcaag agattcgccg cgaccagacg caagggttac agcgaatagg
     3181 tagcgtaagc acgatggttc ggggttctcc ggatgacgca ttagcagaaa tcagagcaaa
     3241 agctgtcgct gcaaaagctg attattacgt tgtcgtcatg gtggacgaaa ccatcgtgac
     3301 aggacagtgg tattcacaag ccattttgta ccgtaaataa atcagacaac ttacatagtc
     3361 agctttacat tgctttgcgt ccctatgcgt ttacctgtgc acaataaatt accggctgga
     3421 agcctgggcg tttattaccg cgatggaatg ccctgcaaca tgtggggaaa cgaaaaatgg
     3481 agctgacgat gaaacaatta cttgcctcac cctcgctgca attagtgact tatcctgcga
     3541 gcgccacggc acagtctgca gaattcgcca gtgctgactg cgtgacgggc ctgaatgaaa
     3601 taggtcagat ctcagttagc aatatttcag gggatccgca ggacgtggaa cgtatcgtgg
     3661 cgttaaaggc cgatgaacaa ggtgcgtcat ggtaccgcat tattaccatg tacgaagacc
     3721 agcagcctga taactggcgc gtacaggcga tactgtatgc atgagtgtca tctacagcaa
     3781 atgaacaatg ccggatgcgg cgcgagcgcc ttgtccggcc tacaattttc acatatttca
     3841 ttgagttaat cgaaaccagc gtcgcatcag tcgtatacgt ttacaaacac actccccccg
     3901 tcgcccgcag caataaatca ttctggatct gctctgacaa ccgtacgccg ccgcgcgtat
     3961 ctaacatcac ctgacaccac gcctgcgcca ttggcggaga agcatatttc aacatttgcg
     4021 caccgcagcc cagcaggaat agctgatgag taatctctcg ccccagttct tcagctggct
     4081 tacgcagctg ctgctgtaaa cgacgaaccg cgcgatcaaa atagcgatcc tgccctttca
     4141 cttccacaaa tgcttccgac aataagtcgt atacgcccgc ttgcttattg agaacgcgca
     4201 acacatccag gcacataata ttgccggaac cttcccaaat actgtttacc ggcatctccc
     4261 ggtaaagccg cggcagctcg ctctcctcgc aataaccaat gccgcccagc acctccatcg
     4321 cttcggccac aaacggcata cctcgtttgc agatcacaaa ttttgccgca ggcgtaaata
     4381 aacgcgccca cagggcttct ttggcatcgg cgcgccggtc ccacgctcgc gcaagacgaa
     4441 acagcaacgc cgtttgccct tcaagctgaa gtgccatgcg acttaagaca tgacgcataa
     4501 ggggctgttg gatcaatgga ttaccaaaaa catggcgttg atgtgcatga taaatcgcca
     4561 gcgaaaacgc acggcgcatc atggcatggc tacccagggc gcaatcaaaa cgcgtcatcc
     4621 cccccatttt caggatcaga cgaattcctt ccccttcctg ccccaataac cagccaatgg
     4681 catcctgaaa ctccacttca caactggcgt tagagcgatt acccagctta tctttcagcc
     4741 gttcgaggcg aatcgcgttg cgttgcccgt caggcaaaaa gcgcggcaca aaaaaacagg
     4801 acagaccgcc cgtggtctgc gccagcacca gatgcgcatc gctttgcgga accgagaaaa
     4861 accatttatg ccccaccagc cgataagagc catcttccaa acgctctgca cgggtggtgt
     4921 tgctcatgac atcggaacca ccctgctttt ccgtcattcc catgccaatc aacaaaccgc
     4981 gtttttgccc acctggcaat aagtgagaat cgtagcgatc gctcagcagc ggcgtggtcc
     5041 agtcctgaaa cggcgtgggt aacatctgta acaacaatgg cgtggcggca aaggtcatcg
     5101 ttattggaca taacgaccca gcctcaacct gcgcatgtaa cataaaacgc gccgcgcgcg
     5161 ccacaaatgc gccggagcga gcgtcttctt cccaggcaag attgtgcacc cgattggtac
     5221 ataacacctg catcagcagg tgccatgcgg gatggaaacg aacatcgtcc agacgccgcc
     5281 cctgggcatc gtagcgcaac agttcaggcg ggtttacatt cgccagccgc cccagttcaa
     5341 gggattcagc cgttcctaac tgctgaccaa tactggctag aaaatcgcta tcccagccag
     5401 caccttcacg cgttaccgct tcgcaaagcg cgccatcaga caggtataag ttgctgttat
     5461 ttaatggtat aggttgatta aaaacggtgt gagtttgcca gtgcactgtg tctccctcca
     5521 tcaatggcag tcaccattag tatggccact gccatggatt catgacagaa agagattgct
     5581 aaaacattct tatccagcaa tgtaatgcgg aacgaaacgt gaggtgtctt tggtgatcag
     5641 tgtgttatct tcacggatcc ccattccgca cgcttcatca tcgacaatcc agctatcgat
     5701 gagtgtgtag ctatcgccaa atcgcggcag aggttgaaac gcctggtaga tcatcggttc
     5761 atcggcgtaa tcaccatcag cgtggtcaac gacgttattc tgaccgtcga aaatggtgac
     5821 gtttccgcct tcgcgcgagt agatcggttt acgcacatag ctttcgccag cggcaatctg
     5881 cgatttttca ccctcaaacc aggatgcaag aaggttagga tgaccaggga agaagcgcca
     5941 aagcagcggc attagccctt tattactcaa gatacttttc cataacggct ccacccattg
     6001 ctcgcgacgc ttgcacagca gcggaccgtt atcgtcacgc atcatccact ccagcggata
     6061 cagcttaaat gcacgctgga tgacattatc atcaagatcg gtcagtacac cgccgacgcc
     6121 caaaccgaga tcttcaatgt agataaaccg cgactcctgc cctgcctgct gggcgcagtc
     6181 ctgcaagtac agcacggtag tcctgtcttc atcggtgtcc tgacagcagc agaaataaaa
     6241 cggctcccgg ctataaagct cgctaaagcg tgaaatcagg cgttcctgaa tggcattgta
     6301 ctgatcggca tcacgcggaa taatgccgct gcgccgggca tcctccagcc acaaccactg
     6361 gaaataagcc gactcataca acgaagtcgg cgtatcggcg ttgtactcca gcagcttcac
     6421 cggcgcattg ccacaccagg caaaatccat ccggccatac agcgaaggat cgctggcgtg
     6481 ccagctttcg gcgatcacat cccagtacaa cggcgggatc gccagttgcg tcaggatctc
     6541 ttcgtcttta accgcgcgat ccaccacctc aaggcacatc tgatgcagtt ccgcagttgg
     6601 tttttcgatc tgctcttcaa tctggcgcag agtaaaacgg taagcccgac tctcgtccca
     6661 atagatttca ttgtcgatga tatgaaagtc gaaaccgttg tcagcggcaa tttggtccag
     6721 atcccgccgc acaggaacgt tgtgtctcag catgattagc ctccccaatg cccacgggcg
     6781 ctggaagaac gaccatagcc gccgcgcgaa acggttgacg cttttttggt ggtaaagccg
     6841 cctgaagagt aagactcttt tttgccagaa ccggagcgcc aggagtaatc accggaagtg
     6901 ttgcgccaga cggggcggga agcaaatgag gatccgccgc tgttatagac gaacggctca
     6961 tcgcgatctt tacgaataac ccggcttaac aaaaatccag aaacgaccgg gatccaactc
     7021 tgttcaacat tgtcgtagta ggaattttcg tacttagact gacagttctg ctgagtcata
     7081 ttcttcggaa catcggcata aaatgccgtt ttggcgttgt tccagccacg cgcgcaaatg
     7141 tcggcattat taccgtcatc aatacaatcc tgcactgtcg cgtaaaacgt tccgtcgccg
     7201 tcgttatcga catcgctact atcgccacaa cctttcaata cgaaaaaagc ggcaccgccc
     7261 attagcgcca acgttaagta tttttgtgcg tagcgattgc gacgcggttc aaaaggatta
     7321 ttcggtctac caa
//
LOCUS       DAZPAC010000207         7264 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZPAC010000207 DAZPAC010000000
VERSION     DAZPAC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.208"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /gene="folK"
                     /locus_tag="ACHUPM_003586"
     CDS             complement(<1..458)
                     /gene="folK"
                     /locus_tag="ACHUPM_003586"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR0871264.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRA"
     gene            complement(455..1852)
                     /gene="pcnB"
                     /locus_tag="ACHUPM_003587"
     CDS             complement(455..1852)
                     /gene="pcnB"
                     /locus_tag="ACHUPM_003587"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR0871265.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1912..2808)
                     /gene="gluQRS"
                     /locus_tag="ACHUPM_003588"
     CDS             complement(1912..2808)
                     /gene="gluQRS"
                     /locus_tag="ACHUPM_003588"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR0871266.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2875..3330)
                     /gene="dksA"
                     /locus_tag="ACHUPM_003589"
     CDS             complement(2875..3330)
                     /gene="dksA"
                     /locus_tag="ACHUPM_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR0871267.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3508..4212)
                     /gene="sfsA"
                     /locus_tag="ACHUPM_003590"
     CDS             complement(3508..4212)
                     /gene="sfsA"
                     /locus_tag="ACHUPM_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR0871268.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            complement(4227..4757)
                     /gene="thpR"
                     /locus_tag="ACHUPM_003591"
     CDS             complement(4227..4757)
                     /gene="thpR"
                     /locus_tag="ACHUPM_003591"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR0871269.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4831..7260
                     /gene="hrpB"
                     /locus_tag="ACHUPM_003592"
     CDS             4831..7260
                     /gene="hrpB"
                     /locus_tag="ACHUPM_003592"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR0871270.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1653 a         1895 c         2038 g         1678 t
ORIGIN      
        1 aatgctcttg tatgtaagac ttcacgcaac gtctccccat caggaaacgc cagctccggt
       61 gcaatctcaa acagcggcca cagcataaat ccacgattct tcatatcgta gtgcggaacg
      121 gtcaggcgtt cggtatttat cacttcatta ccaaacagca tgatatcgag atccagcgtg
      181 cgtggtcccc agcgttcagc tttacgaaca cgaccctgtt gcagttctat gcgctgtgta
      241 tggttgagta actcttccgg ggcgagcgaa gtttcaagcg ccacggcggc gttgaggtaa
      301 tcgggttgat cttgcggccc cagcggtgga gtgcggtaaa acgaagaaac ggcaagaatg
      361 tggctttcag ggatatcgcc taatgctttc agggcagcat tgacctgctc cagcggagag
      421 gccagattgc tgcctatggc aatatacgcc actgtcatgc ggtaccctca cgacgcggtg
      481 cgcgtttgcg tggacgacga gtacggcgac gcggtgacgg ctcttcatcc aactcgttaa
      541 gcatcccttt ttgatctggt ggcgcggaaa cctggaactc accccaccat ttcaccagac
      601 gctgcagttc agcgttacgt tcaacttcag ctcgcaaggc caacaggtca taagccgcac
      661 ggaacttagg atgctccagc agtttccatg cgcgtttacc ctgacgtcgg gacatacgca
      721 actgcaactg ccagatatcg cgggttaacg tcgtcaggcg tttcgggatt gccagtgaac
      781 ggcaggcttc gtccagcacg tcgttcatcg ccagcgcgaa agcgtcgtga taggtcaggc
      841 cgctttcctg ggcgatcttc tgtgccgtct ccagcagtgg gtaccagaac atggcggcaa
      901 acaagaacgc cgggttcacg cgcatatcgt tatggatacg cgtatcggta ttcttcagca
      961 cctgttcaat gatccgctcc atcgggctgt cgccattttc cgtgaagtag cgggtaatgg
     1021 tcgggaacag cggctggaac agatgatatt cacacaacag cttataggtt tcgtaaccgt
     1081 agcccgcttg tagcagttta agcgattctt caaacaggcg tgccggtggg atatcgttca
     1141 gcagggtagc gaggcgaggg atcggttctg cggtttccgg gctgatgcgc atacccaatt
     1201 tggcggcaaa acgtaccgcg cgcagcatcc gtaccggatc ttcacggtag cgcgtttccg
     1261 ggttaccaat cagacggata acgccgtcct tcagatcctt cataccgcca acgtaatcac
     1321 ggacggtaaa atccgctacg ctgtaataga ggctgttgat agtgaaatcg cggcgctggg
     1381 cgtcttcttc gatggagccg aaaatgttgt cgcgcagcaa catgccgttt tgcccgcgtt
     1441 gggaggtcgt gcggtcgctg acgttacctt cgtggtgtcc acggaaggtc gcaacttcga
     1501 taatctccgg gccaaacatt acatgagcca gacggaaacg gcgacccacc aggcggcagt
     1561 tacggaacag tttgcgcacc tgctcaggcg tggcgttagt ggttacgtca aaatctttcg
     1621 gctttttgcc aagtaacagg tcgcgcacgc cgccgccaac cagccaggct tcgtatcccg
     1681 ctttgttgag cctgtacatt accttcaggg cattttcact gatatctttg cgggaaatag
     1741 catgctgctc acgcgggatc accgtcacct gtggacgggc gactgcctgt tcagcctcgc
     1801 tttcctcgcg gcttagcacc ttgcggcaaa aattagcgac tcgggtaaaa atagtacacc
     1861 tcggtagtgt caaacatcat tcaggacaaa aaaatagcgg ctaatcatag ctcagcatga
     1921 cgcatttgag aatgttgaat ttacaattgc cgactcgggc acggcggtca gcgtccagtt
     1981 tttgactgct gactgaagga tttgttcgac gctgaaatcc tgccagtgtg cttctgcctg
     2041 ctgacccaga aattgaagtg ccgcgattag taccggacgt ggatcgcctt tcggcaacgc
     2101 aggcgcatga ttctgcttgg aaagtttagc gccttgtgga ttaagcgcca gcggcagatg
     2161 aatgtaatct ggcactttcc agccaaaaag ctggtacagc gagatttgcc ttactgttgg
     2221 ttcaatcaga tcagccccac gcactatttc tgtaacgccc tggaaatgat catcaaccac
     2281 aacagccagg ttgtaggcga acaacccatc acggcgatga atgataaaat cttcccgtgc
     2341 cagtttttcg tcggcgtgaa taatgccgcg cagctggtca gtaaattgcg tgaccggatg
     2401 ctgctggcgg atacgcactg cggcattgtc tggtccatga tgcaacaccc ggcaatgacc
     2461 gtcgtaaata ccgccaatgc tttgaatacg cgcacgcgta caggtgcagt aataacttag
     2521 tccttgttca tgtaaccagg cgagtgcttc acgataggcg tcgtgacgtt gcgattgcca
     2581 gagaacatcg ccgtcccagt gcagaccgta atgttccagc tggcgcagga tagtttctgc
     2641 ggcaccggga acttcacgag gcgggtcgat atcttctatg cgtaccagcc agcgaccttg
     2701 ccgggcgcga gcctgcaaat agctgccgag cgcggcgatc agagagccaa aatgaagctc
     2761 gccggaagga gagggggcga agcggccaat atactgtgtg tctgtcatct ctttgaacaa
     2821 aaaataaggc gggagcattt cccgcctgtg gtaaacgtga tggaacggct gtaattagcc
     2881 agccatctgt ttttcgcgaa tttcagccag cgttttgcag tcgatgcaca gatcggctgt
     2941 cgggcgcgct tccagacggc gaataccaat ttcaacaccg caggattcgc agtagccgaa
     3001 atcttcgtct tccacttttt tcagcgtctt ctcgatcttt ttgatcagct tacgctcgcg
     3061 atcgcggtta cgcagttcga ggctgaactc ttcttcctgg gctgcacggt ctaccgggtc
     3121 cgggaagttg gctgcttcat cctgcatatg tgtaacggtg cgatcgactt catccctgag
     3181 ttgattacgc catgcttcca gaatacgacg gaagtgcgcc agctgggctt cattcatata
     3241 ctcttcgccc ggcttctcct gatatggttc caccccagcg atggcgagaa tactcaggga
     3301 cgatgtttta cggttttgcc cttcttgcat gttgcttctc cttaacacgc actatcgatc
     3361 cccatgttcg ggggaaaaat gaggccgcta taaatagcag atgcttttcc ggatagcaat
     3421 tatctaaacg taacacttga caactgtgtg aggaaaagcg tatttgcgca cgcgaccaga
     3481 atgtaaatta accagttact tactttacta caatgtaacc gacagtgatt ttttaagagc
     3541 catgccttca gcagaaattt ccgctttgta agccagaatt tctacccccc tctgttgagc
     3601 ttctgacaat agttgcgcgt atttctcatc gatgtggcgc gcgggtgaaa accgtgtaat
     3661 ggctgaatgc agcacggcga aaaagataac cgcacgctgg ccttcagccg ctacgttcat
     3721 caactcccga aggtgtttct gacctcgttc agtgaccgca tcgggaaaat atccctgttc
     3781 gttctccgct aacgtaaccg atttcacttc aatatagcag tctggacgcg aatccgcctg
     3841 caacataaag tcaatacggc tgcgttctgc gccgtatttt acttcgcttt tcagcgagct
     3901 atagcctgac agttctgaaa ttgattcatt aaggatagcc tctttcgtca acctgttagc
     3961 ccaaagcgta ttgacgcaaa taaatgcgcc gctctggctt tgagttagtt cccaggtgtg
     4021 tgggtatttc cgtttggtgt tgtctgaagt cgaataccag acggtatcgc cacgcgttgc
     4081 acaaccggtc atcgcacccg tattcgggca gtgtagcgtt aattcgcgac catcgggtgt
     4141 gatcacatcg gctaaaaaac gtttgtaacg ctgaattagc gtcgcgcgct gtagaggggg
     4201 agagaattcc attgcgacaa tccttgttat tgcgttagcg cccagcgttt tagcggcgtg
     4261 tagcgtgtgc gtccacgggc aaacgaggag gcgtaaaggg tgaactccgt caccgcataa
     4321 gaccagttaa aacctggcgg cgggattgtc accgcctcgc tggcgtcgcg caataaggta
     4381 atatgtggat gaaacggacg attgctttga aaacaaccgc tgcgggcagc ctgtgaacgg
     4441 agcatattcg ccagctggat taagccgcgt ggcggctgac gcatccctaa ccacaccaca
     4501 cgcgaacgca gccattgtcc ggcgtcatca agcgtgagtg tgaaaccagg ttgacgaatc
     4561 cgtccggcta aaagagaaag cgccttctct ttctcggcgc tcacttcgcc taaaaatgcc
     4621 agagtcagat gcaaattatc ggcggcgacc ggacgtcccg cctcaggtgg gaagtgtgtg
     4681 gcgcgccagt ggataatctg ttcgcggatt tctgcaggta agtcgatagc aaagaacaga
     4741 cgttgcggtt cagacatgtg aggcactcgg ttatgaatta ccgcgatgct acaatgtggc
     4801 gcgaagaatg ttaaccctct ggagcgtttt gtgtcgtcgt tgcccgttgc tgccgtctta
     4861 cctgaattac ttaccgctct cgattgtgcg ccgcaggtat tattaagtgc gccgaccggg
     4921 gccgggaaat caacctggct gccgctgcaa ctgctggcgc atcccggcat taacgggaaa
     4981 attatcctgc tggagccgcg tcgtctggcg gcacgtaacg tcgcgcaacg gctggcggag
     5041 ctgcttaacg aaaagccagg cgataccgtt ggctaccgga tgcgtgcgca aaactgcgtc
     5101 gggccaaata cccgtctgga agtagttacc gaaggcgtgc tgacgcggat gatccagcgt
     5161 gacccggagt tgagcggtgt tggattggtg atccttgatg aatttcatga gcgcagcttg
     5221 caggcggatt tggcgttggc gctgttactc gatgtgcaac aaggtctgcg tgatgacctt
     5281 aaactgctga ttatgtcggc tacgctggat aacgaccgct tgcagcaaat gctgccagaa
     5341 gcgcccgtcg tcatctcaga agggcgctcg tttccggttg aacgccatta tttaccgctg
     5401 cccgcgcatc agcgttttga cgaagccgtt gcggtagcca ccgccgaaat gctgcgtcag
     5461 gaaagcggat cattactgtt atttttacct ggcgtcggag aaattcagcg tgtgcaggaa
     5521 caactggctt cgcgcatcgg cagtgatgtg ttgctctgcc cgctgtatgg cgcgttgtcg
     5581 ctgaacgatc agcgaaaagc gatcctcccg gcaccgcaag ggatgcgcaa agtggtgctg
     5641 gcgaccaata ttgctgaaac cagtttaacc attgaaggta ttcgtctggt ggtggattgt
     5701 gcccaggagc gtgtggcgcg ttttgagccg cgcacggggc ttacgcgact gattactcaa
     5761 cgcgttagcc aggcatccat gacgcagcgt gccgggcgcg ccgggcgtct ggagccgggt
     5821 atctgcctgc atttaatcgc caaagaacaa gcagaacgcg ctgccgcgca aagtgaaccg
     5881 gagatcttac aaagcgatct ttccggtttg ctgatggaat tactgcaatg gggatgcagc
     5941 gatccggcgc agatgagctg gctggatcaa ccgccagtag tgaatctaat ggccgcgaaa
     6001 cgtctgttac aaatgctggg ggcgctggac ggtgaacggc ttagtgcgca agggcaaaaa
     6061 atggcagcgc tgggtaacga tccgcgttta gcggcaatgc tggttagcgc gaagaacgac
     6121 gacgaagctg ctaccgcggc aaaaattgcc gccattctcg aagagccgcc actgatgggc
     6181 aatagtgacc tgggcgtggc gttttcgcgc aatcaaccag cctggcagca acgtagtcag
     6241 caactgttaa aacgcttaaa cgtacgcggc ggtgaggcag acagttcgct tatcgcgccg
     6301 ctacttgccg gggcgtttgc cgatcgcatt gctcatcgcc gtgggcaaga tggacgctat
     6361 caactggcaa acggcatggg agcgatgctc gatgccgacg acgcgctaag ccgccacgaa
     6421 tggttgatcg caccgttatt attgcagggc agcgcctcgc cggatgcgcg gattttactg
     6481 gcgctaccgg tcgatattga tgaattagta caacgctgtc cgcagctggt gcagcaatct
     6541 gacaccgtgg aatgggatga cgcgcaaggt acgctgaaag cctggcgtcg gctacaaatc
     6601 ggtcagttga cggtgaaagt gcagccgctg gcgaaaccgt cagaagacga gttgcatcag
     6661 gcgatgctta atggcatccg tgataaaggt ttaagcgtgc tcaactggac ggcggaagcg
     6721 gaacagctac gcttgcgttt gttatgcgcc gcaaagtggt tgccggaata tgactggcca
     6781 gcggttgatg atgaaagttt attggcagcg ctggaaacgt ggctgctgcc acatatgaca
     6841 ggcgtacatt cactacgcgg cctgaaatca ctggacattt atcaggcact acgcggatta
     6901 cttgattggg gaatgcagca acgtctggat agtgaattgc ctgcgcatta cactgtgccg
     6961 acgggaagcc ggatcgccat tcgttatcat gaagataacc cgcccgcact ggcggtgaga
     7021 atgcaggaga tgtttggtga ggccactaat ccgactatcg cccagggggg cgtgccgctg
     7081 gtgctggagt tgctttcacc cgcccaaagg ccactacaaa tcacacgaga tttgagcgcc
     7141 ttctggaaag gagcgtaccg tgaggtgcaa aaagagatga aagggcgtta tcccaaacac
     7201 gtctggccgg acgacccggc aaacacggca ccgacgcgac ggacgaaaaa gtattcgtaa
     7261 ggtt
//
LOCUS       DAZPAC010000208         7239 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZPAC010000208 DAZPAC010000000
VERSION     DAZPAC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.209"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..337
                     /locus_tag="ACHUPM_003593"
     CDS             <1..337
                     /locus_tag="ACHUPM_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0871271.1"
                     /translation="ADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGK
                     LGGVADTGGSGMVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARD
                     GGRLSGAYR"
     gene            337..666
                     /locus_tag="ACHUPM_003594"
     CDS             337..666
                     /locus_tag="ACHUPM_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0871272.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVKIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            676..1374
                     /locus_tag="ACHUPM_003595"
     CDS             676..1374
                     /locus_tag="ACHUPM_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR0871273.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            1379..2122
                     /locus_tag="ACHUPM_003596"
     CDS             1379..2122
                     /locus_tag="ACHUPM_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0871274.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEVT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            2059..2661
                     /locus_tag="ACHUPM_003597"
     CDS             2059..2661
                     /locus_tag="ACHUPM_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR0871275.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            2722..6201
                     /locus_tag="ACHUPM_003598"
     CDS             2722..6201
                     /locus_tag="ACHUPM_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0871276.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNSYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAIGRASDDAEGYLNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            6269..6868
                     /locus_tag="ACHUPM_003599"
     CDS             6269..6868
                     /locus_tag="ACHUPM_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR0871277.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDGGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            6932..>7239
                     /locus_tag="ACHUPM_003600"
     CDS             6932..>7239
                     /locus_tag="ACHUPM_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0871278.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     "
BASE COUNT         1637 a         1727 c         2315 g         1560 t
ORIGIN      
        1 tgctgatttg agtcgctaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc
       61 aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgcgg
      121 tgctgatggt aagctggggg gtgtggcgga tactggtggt tcaggcatgg tgatgtttgc
      181 cccgcagtac aacattgaga tcaataacga tggcacgaac gggcagatag gtccggctgc
      241 cctgaaggtg gtttatgacc tcgggaaaaa agcggccgcg gactttatgc aacagcaggc
      301 ccgtgatggt ggccggttaa gtggagcata ccggtaatgg agacgtttca ctggaaagtg
      361 cgcccggata tgaatgtggt atcagagccg aaagtggtga cagtgaagct gggcgatggt
      421 tatgaacagc gtcgtgcggc gggactgaat aaccagttgt cgacttacag cgtgaagata
      481 cgtgttcgta aaggtgaaca cccattttta aaagcctttc tggaacggca cggtggcgtc
      541 cgcgcatttc agtggacgcc accttatgac tggaagccga tcagggtggt ttgtcgtaaa
      601 tggtcggcaa gcgtgggggc gttgtgggta accataacgg cagattttga acaggtcgtg
      661 gcataggagg ctctgatgca ggatattcca caggaaacac atcatgagac gacacgcctc
      721 actcagtcag cccaggtggt gctctgggaa atcgatctga cagaggtcgg tggtgaacgt
      781 tattttttct gtaatgagca gaacgaaaaa ggtgagccgg ttacctggca ggggcggcag
      841 tatcaggcat accccattca ggggacggga tttgaactga acggcaaggg cagtgctgcc
      901 cgtccgacac tgacggtttc taacctgcac ggaatggtca ccgggatggc ggaagacctg
      961 cagagtctgg tcggcggaac ggtggtcagg cgtaaggttt acgcccgttt tctggatgcg
     1021 gtgaacttcg tcaacggaaa cagcgacgcc gatccggaac aggaggtgat cagccgctgg
     1081 cgcatcgagc agtgcagcga actgagcgcg gtcagtgcct cttttgtgct ggccacacca
     1141 acggagacgg atggcgcggt tttcccgggg cgtatcatgc tggcgaatac ctgtatgtgg
     1201 acttaccgtt ctgatgagtg tggttacacg ggcagggcag tggctgacga gttcgacaaa
     1261 ccaacgacgg atatccggaa ggacaaatgc agcaagtgta tgcgcgggtg tgagttgcgc
     1321 aacaataccg gtaatttcgg cggtttcctt tccatcaata aactttctca gtaaatccat
     1381 gacacagaca gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg
     1441 tggcttcgtg gtaagaacgc cggagggaga caggtatctt cccagcgaga atatctccgg
     1501 tgagccggag gaacggttcc ggatggctcc ggaggactgg ctgcgggcac aaatgcaggg
     1561 tgagattgtg gcgctggtcc acagtcatcc cggtggtctg ccctggctga gtgaggctga
     1621 ccggcggctg caggtgcaga gtgatttgcc gtggtggctg gtctgccggg gggcgattca
     1681 caagttccgc tgtgtgccgc atctcaccgg gcggcgcttt gagcacgggg tgacggactg
     1741 ttacacgctg ttccgggatg cttatcatct ggcagggatt gagatgccgg attttcatcg
     1801 cggggatgac tggtggcgta acggtcagaa tctctatctt gacaatatgg aggtgacggg
     1861 tttttaccgt gtcgcactga cagaggcaca gccgggcgat gtgctgctgt gctgttttgg
     1921 ttcatcggtg ccgaatcatg ccgccattta ctgcggtgac ggcgggctgc tgcaccatat
     1981 tcctgaacaa ctgagcaaac gagagaggta caccgacaaa tggcagcgac gcacacactc
     2041 cctctggcgt caccgggcat ggcgcgcatc tgcctttacg gggatttaca acgatttggt
     2101 cgccgcatcg accttcgtgt gaaaacgggg gctgaagcca tccgggcact ggccacacag
     2161 ctcccggcgt ttcgtcagaa actgagtgac ggctggtatc aggtgcgcat tgccgggcgt
     2221 gatgcaggtg aaaccgaatt gtctgcccgt cttaatgagc cgctggcaaa tggtgcagtg
     2281 atccacatag taccgcgtct ggtgggagct aaaagtggcg gtgtgtttca ggcggtgctg
     2341 ggggcggctg ttatggcggt tgctatatgg atgccggggg taggaattat ggcgagtaat
     2401 ctgctgtttt ctctcggtgc cagtatgacg cttggcggtg ttgcacagat gctggcaccg
     2461 aaagccagaa ctccccgtac acagacaacg gataacggta agcagaacac gtatttctcg
     2521 tcactggata acatggttgc ccagggcaat gttctgcccg ttctgtacgg tgaaatgcgc
     2581 gtggggtcac gtgtggtttc tcaggagatc agcacggcag acgaagggga tggtggtcag
     2641 gttgtggtga ttggtcgctg atgcaaaatg ttttatgtga aaccgcctgc gggcggtttt
     2701 gtcgtttatg gagcgtgagg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa
     2761 gcgaaggaca acctgaaatc cacgcagttg ctgagtgtga ttgatgccat cagcgaaggg
     2821 ccggttgacg gtccggtgga tggattaaaa agcgtgctgc tgaacggtac gccggtcctg
     2881 gacagcgagg ggaagacaaa cttttccggt gttacggtgg tgttccgtgc aggtgagcag
     2941 gagcagacac cgccggaggg ttttgaatcc tccggctccg agacggtgct gggtacggaa
     3001 gtgaaatatg acacgccgat cacccggacc atcacgtcgg caaacatcga ccgtctgcgc
     3061 ctgaccttcg gtgtgcaggc actggtggaa accacctcaa agggtgaccg gaatccgtcg
     3121 gaagtccgcc tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc
     3181 accattaagg gcaaaaccac ttcgcagtat ctggcctcgg tggtggtggg taacctgccg
     3241 ccgcgcccgt ttaatatccg gatgcgcagg atgacaccgg acagcaccac agaccagctg
     3301 cagaacaaaa cgctctggtc gtcgtacacc gaaatcatcg atgtgaaaca gtgctacccg
     3361 aacacggcgc tggtcggcgt gcaggtggat tcagagcagt tcggtaacca gcaggtgagt
     3421 cgcaattatc atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg
     3481 cggcaataca gcggtatctg ggacggaacg cttaagccag catacagcaa caacatggcc
     3541 tggtgtctgt gggacatgct cactcatccg cgctacggca tggggaaacg tcttggtgcg
     3601 gcagatgtgg acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtgacg
     3661 gacggatttg gcggcacgga gccgcgcatc acctgtaact cttacctgac cacacagcgc
     3721 aaggcgtggg atgttctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac
     3781 ggacagatgc tgacgttcgt gcaggaccga ccgtcggata aggtgtggac ctataaccgc
     3841 agtaatgtgg tgatgccgga cgatggcgcg ccgttccgct acagcttcag cgccctgaag
     3901 gaccgccata atgccgttga ggtgaactgg attgacccag ataacggctg ggagacggcg
     3961 acagagcttg tggaggatac gcaggccatt gcccgttacg gtcgtaatgt cacgaagatg
     4021 gatgcctttg gctgtaccag ccgggggcag gcacaccgag ccgggctgtg gctgattaaa
     4081 acggaactgc tggaaacgca gaccgtggac ttcagcgtgg gcgcagaagg gcttcgccat
     4141 gtaccgggtg atgtcattga aatctgcgat gatgattatg ccggtatcag caccggtggt
     4201 cgcgtactgg cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg
     4261 ccgtcctccg gtaccacgct gataagcctg gttgacggaa gtggcaatcc ggtcagcgtg
     4321 gaggtccagt ccgtcaccga cggcgtgaag gtgaaagtga gccgggttcc tgacggcgtt
     4381 gcagaataca gcgtgtgggg gctgaagctg ccgacgctgc gccagcgtct gttccgctgc
     4441 gtgagtatcc gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg
     4501 gaaaaagagg ccatcgtgga taacggggca cactttgacg gtgaccggcg cggcacggtg
     4561 aatggtgtca cgccgccagc ggtgcagcat ctgaccgccg aagtcaccgc agacagcggg
     4621 gaatatcagg tgctggcgcg atgggacacg ccgaaggtgg tgaagggtgt gagcttcctg
     4681 cttcgcctga ccgtggcagc ggacgacggc agtgagcggc tggtcagcac agccaggacg
     4741 acggaaacca cataccgctt caggcagctg gcgctgggga attacagtct gacagtccgg
     4801 gcggtaaatg cccgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgcggca
     4861 ccggcagcgc ctgtcactat tgaactgata ccggggtatt ttcagataac ggcggtcccg
     4921 aaactggctg tatatgaccc gacggtgcag tttgagttct ggttttcgga aaagcggatt
     4981 gcggatatca ggcaggttga aaccagcgcc cgctatcttg gcacggcgct gtactggata
     5041 gccgccagta tcaatatcag gccgggccat gattattatt tttacgttcg cagtgtgaac
     5101 accgttggca aatcggcatt tgtggaggcc atcggtcggg cgagcgatga tgcagaaggt
     5161 tatctgaatt tttataaagg gttgatcaat aaaacgcatc tcggcaagga gttgtggacg
     5221 cagattgata acggtcagct tgcgccggac ctgactgaaa tcaggacgtc cattacgaat
     5281 gtcagcaatg aaatcacgca aaccgtcaat aaaaaactgg aaaatcagag tgctgcaatc
     5341 cagcagatac agaaagttca ggttgataca aataataacc tgaacagcat gtgggctgtg
     5401 aagctgcaac agatgaagga cggacgcctt tatattgcgg gtatcggtgc cggtattgag
     5461 aatacgccag caggaatgca gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt
     5521 aatcctgcga atggcaacac aaagccgatg tttgttggtc agggcgatca gatattcatg
     5581 aacgacgtgt tcctgaaatg cctgacggct ccgaccatta ccagcggcgg taatcctccg
     5641 gcattttccc tgacacctgg cggacggctg acggcgaaaa atgccgatat cagcggtaac
     5701 gtgaacgcga actccgggac gctcaacaac gtcacgatta ataagaactg tcgggttctg
     5761 ggaaaattgt ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaacctttc
     5821 ccacgggact cccgggcacc ggagaggtgg ccatcaggga ccattaccgt cagggtttat
     5881 gacgatcagc cgtttgatcg gcaaattgtt attcccgcgg tggcgtttcg cggtgctaaa
     5941 catgagcgga agaataacaa tatttattcg tcatgccgcc tgatagtgaa gaaaaacggt
     6001 gctgaaattt ataaccgaac gaccctggat aatacgctga tatatacggg tgttattgat
     6061 atgcctgccg gtcacggtca catgacgctg gagttttctg tatcggcatg gctggtaaat
     6121 ggctggtatc ccacagcaag tatcagcgat ttgctggttg tggtgatgaa gaaagccact
     6181 gcaggcatca cgattagctg aattttataa cccagatacg ggcaccagaa atggtgcctt
     6241 ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca ttttgtccgc
     6301 agccatctgt ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct
     6361 gagcgccggg tatcttcaga cccatactga tatgccaggc agcgatgacc tgaagggcat
     6421 taacgtgaaa taccgttatg agtttacgga cacgctgggg ctgattacgt cattcagtta
     6481 tgccaacgct gaagatgagc aaaaaacgca ttacagcgat acccgctggc atgaggattc
     6541 cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga atgaatggtt
     6601 cagcgcgtat gcgatggcgg gcgtggctta cagccgtgtg tcgaccttct ccggggatta
     6661 tctccgcgta actgacaaca aggggaaaac gcacgatgtg ctgaccggaa gtgatggcgg
     6721 tcgccacagc aacacgtctc tggcgtgggg ggctggcgtg cagtttaacc cgaccgaatc
     6781 cgtggccatt gaccttgctt atgaaggttc cggcagtggt gactggcgca ctgacggttt
     6841 catcgtgggt gtcggttata agttctgatt agccaggtaa cacagtgtta tgactgcccg
     6901 ctggttcagg cgggcttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa
     6961 agatggggcg ggtaaacctg tcgtaaattg tgcgattgaa ctgcgggcca gaagaaccag
     7021 tccgaccgtt gtggcacacg ttgttgccac ttgcgtgacg gacaataacg gtgcttatgt
     7081 gattgaggct gagccggggt attacgaggt tgcgcttcac tgtaacggct ggcagccaac
     7141 ccgtgtcggg gatattgatg tggcaccgac tgatgcaccg gggacactga acgcgtttct
     7201 gaatgcacca aaggatggtg atttacgtcc ggaggtgat
//
LOCUS       DAZPAC010000209         7137 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZPAC010000209 DAZPAC010000000
VERSION     DAZPAC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.210"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(1..4519)
                     /locus_tag="ACHUPM_003601"
                     /pseudo
     CDS             complement(1..4519)
                     /locus_tag="ACHUPM_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4539..5000)
                     /locus_tag="ACHUPM_003602"
     CDS             complement(4539..5000)
                     /locus_tag="ACHUPM_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR0871279.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5028..6929)
                     /locus_tag="ACHUPM_003603"
     CDS             complement(5028..6929)
                     /locus_tag="ACHUPM_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFR0871280.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1319 a         2187 c         1944 g         1687 t
ORIGIN      
        1 tttatttgac tacgtgataa acgaacccca tccaggaaac ccgtatgccc actaacacga
       61 cgaccatcgc caatatagtc catcaaactg ctgattttag aacttgcagc caatcccagc
      121 ggatcaaccc acccacaagg attcggcaca taccggtaag tctgctcccc gccctgcaac
      181 cccagcggat cactcaccag gtacattccg gtttccggct cgtaataccg gaaccgatta
      241 tagcacagcc cggattccgc atcctgccac tgtcctgcat acagcaggcc ggggtcggct
      301 tccgggtcca gttccccgcg cgggtccggg tagccggtgt ctgcgggcag gctgagtgcc
      361 agcccccaca ggctggtctg ccccggtcgc cagaccgtct ttccttcact gttaaagagc
      421 atcagcgggc ggcccgtcag gtcgctcacc gcatgattcg tccgcgtcac ccactgcggg
      481 gccaccgacg gatgcgcctg ccgtacccgg ctgaactgct ggcttatcag ctcaaagccg
      541 ttaaacacca ggtgccttac gctgtacagt ttatcatcgc ggtattcccg gatttccgca
      601 atactgtcgc cgtcccacag atacgtgaag cggattttct tccggtcgca gcgtttctct
      661 gttcgtctgc cgctggcgtc gtggcggtat tcccacagct caccctgtgc gctgcaatac
      721 ccggtcagct ggtcccggct gtcccaccgg aaccgctctg tttccggtcg gtagccgtca
      781 cggtgtctgg tgcggctgac catccggcct gcggcgtcat agtcatactg cgtgtttccg
      841 gcctgtttca gccggtggcc tccggcatac tggtcagtct cttcacttat ccggtgcctg
      901 cccgcagact gcgccttcag gtagccgcag ctgtcatagc cataggactc atcgccttcg
      961 ccatagccca gcccggtgcc tgaggctgtc accgccgtga cctggccgtt ccggtccagc
     1021 cgatactccc gccgtgtttc ccgtccgtaa tcttcccggc tggtgacgcc tgacacgtta
     1081 cccgcgctgt cgtaacggta ctcccgtacc agtgagggaa ccagcccgtc ccatgtggct
     1141 tctgccggaa aaaatccggc gcgctgactg acaggctgac cggctgcatt ccagccctgt
     1201 tcacaggcaa aaccccggtt actgtgccgg agcacttcac gtcccgcctc atcacggctg
     1261 aacgtcagcg tctcatcccc cggcagaatg acctctgtca gttctccggc ggcgttgtag
     1321 cggtacgtgg ttttcagcag ggattcaccg gggtgtctgt cgcgttcatc ttccggtggc
     1381 aggatacagc ggatgacggt gtgttcatcg gggtagttgt attcaatggc ccgtcggccc
     1441 tgagtggtga cattcacccg cccgcgttcg tcatagccgg tgcgctgtga tgtgccgtcc
     1501 gggctgaaga tacccgtcag cctgccggat ttgtcatagg catactgcgt tttgccttcc
     1561 ggcgagatga tttctgccag gcggcctgcg gcgtcccggc tgtagaggat ggtttcctcc
     1621 aggccgttaa ccttggcggt acagaggcca tcggcgtcat agcggtaacg ccagaccacg
     1681 ccgccccagt cacgctcttc cgtcacccgg ccgcagttat ccagaacata aagatatgac
     1741 tcaccctgcg ggttgatgac ttctgtcagt tgcagcgtgt ctttatcgta gcggtactgc
     1801 cagcggtggc cttcaccgtc cgttcgcgcc accggaaaat cgaacggacc gtattccatc
     1861 gtccagacaa caccgttgcc gtccgtccag gccagcaggt tgccgtggcg gtcatagcga
     1921 aatccttccg cgccggttcc agcacgttca atgccgctca gtttatggtg ctgctgccac
     1981 tgtaaccgca ctgactgacc gccggggccg cgcatcttca ctggcaggtc gtgaccgctg
     2041 tattcggtgt ggtgttcacg gcccatcacg tcggtgagtg tttccggccg gttgtggtgg
     2101 tcgtactcta tgcccagacg ccgttcatcc tggcggaata ttcccgacag cagcccctga
     2161 gcggtgtatg ccagccgggt ctcttcgccc tgtggcgaca ccagacttac cggcaggccg
     2221 cgttcatcac actgctgtgt ccacgtctgc ccggcagggt cggtgatgtt gctgagcagg
     2281 ccgcgctcat catagcggta ctgccactcc gcgccgtctg ccgccgtgta acggcttaac
     2341 tgaccgtgtg cggcgttgta ttcaaatttc tccacccgcc cggcagggga gtggcgggcc
     2401 agcaggtgat ggaattcatc ccattcatac atggtggtgc tgccgtccgg cgcttcttcc
     2461 cgcagaatat tgccgtcagg atcatagtaa taacggtgaa tgcccttgcc gtccgtcacg
     2521 gtggtgcagc cgtcaccata gtccagcgtg gcgttgtaat agccctccgc cccggtgaca
     2581 ttcacgcacc ggccctgttc atcgtaggtg aaacggctcc acgtctggtc gttatcggac
     2641 cagcggatga tccggtccgc agcgtcgtac tcataaaaca ggtggtagtc cagccgcgca
     2701 tccgcttctg tcagccgccc ccgcgcatcc tgttcgtagg tcgccagcgt ctgtatgtcg
     2761 ttatccgtgc ggtgaatggc tttcaggtag ccgctggcgt ggtacgtcag tttaagccgg
     2821 atcccgtcgg tgtgactgac acccgtgatc cggtactggc tgtcgcgctc aaacgtgatg
     2881 cggttgctgg cggtgtcgct catcccgccc agcatgtaca tccggtcatt ggggctgtgt
     2941 gccaggcggt catcgctggt atcgtcagtc accgggtggc gcagacgcat tcccggaaag
     3001 gcgtcataaa agaagcggct gctcagtgca tccctgtcga acagctccag ataatcatcg
     3061 cggcggcgca gaatgtactg ggcgtgatac gggttaaccg ccgtatcact gtcaccgtcg
     3121 aaggcaaatc tcagcgtggc tccctggctg atgatatcca cccggtttcc ctgttcacgc
     3181 acccaggcgt attcgctcca ggagtcgctc cagcccacgc cggtcaggcc atgcagcggc
     3241 agtacagaac tgtggaaacg ggtgaaggag agggggaggg tctggcccag ggtgaagtcg
     3301 gtacgttcgt cgcagtatgc gccggttacc ggatcgacag gatctgttac ccatctgatg
     3361 actttattgg caatactttt taacgactta cctgttttcc ccagcaacgc gccgagcttt
     3421 gcgcccagca gcttcccgct tttccccagc agtttcccag tttgccgagt ctcgccagat
     3481 tcttcagttt tcccagtagt cccgctggcg gcagaagata gctggctatc gttattcccc
     3541 ccaggatcat tctctgccac ggcgggaact ccggctggat atccagatat gtgacctgct
     3601 caccgccaat aaagacgtcc ggcgaacctc ccacgatcgc cgcactgcac tccagtttgt
     3661 cgcccttgcg cgcggcaggc tgattgttga taaagacgtt gctgctgccc tgggctatcc
     3721 tcggtggttt gttctccttg tcgcagagcg ccgtgtcctc accggcttcc gggaaatcca
     3781 catctttttt gatttcgcca cgcccgccca ccatggtggc aaactccatt aataccggac
     3841 cggcgggaaa cagaagcgat gcgccggcgc tgacgcgatc cataaaaccg gcattgctgt
     3901 ccgtcagggt gataacagcg ttcccccagg ccttcgccag tggtactgct ttttccgctg
     3961 ccgattcaag caggtcagtg gcaatatcac cccatgaggg ttccccctgt gggacttcag
     4021 gtattgctcc aggtggggga ggagggggaa gggtgaccgc tgcacgtgcg gccagctcgc
     4081 cgttaatttt tacgttttca gaaccggtat tgatggcgcc gtccgtgtca caaaagctgt
     4141 ttatccatga ttcaagctgg ttctgtttct cctccacata atcactcgcc ttatagctgg
     4201 catacatcag cgcaggtacc agaaccagcg cataagggcc ggtggcacag gcggccataa
     4261 tcaggcaacc ttctgcgatg gtcactatcg cccccagtac ggcccctgcc agcgccccca
     4321 aaaaactctt gtgctgaatt tcatcgccca gcctggcggc gggggggcca gacttttttg
     4381 atgccaacat ctgtgcaatg gctttgtcgt tggctgcccg tttcatctgg ctggcgacca
     4441 tcgcctgctg tgacagcatt cgcattgcca gcgtaccgcc tgcggtcgct ccctgtggcc
     4501 cgcctggtcc ttcactcatg tgttatccct cgtttatgtc agcgcggctg gtccttttgg
     4561 atgtcattct ccggtggcaa cggggtgaag ctctttatca cctccagcag catcgtcttt
     4621 tgactgttct tcagggataa ggaggtgctg aaagtgaagg tcagcagcgt atctccacgc
     4681 cgctggaata cggctatctg gctaatccgc ccctgattcc cctcatcttc cggaccgttc
     4741 caggaataat ccagcagcca ggccggggcc tctgccagag tgatttcccg gcaggcatgt
     4801 tcggtgtacc catcaagttt gccctggtac agcgccagcg tttcctggta cagctctggt
     4861 gtcagggttc cctgtggaac ggcttcgcgg gtaatgacca gattgatgcc actgttatct
     4921 gtggacacca ggacctgcat tgactgatcc tgccattgag acggcagttg aatgtggcct
     4981 tcattaaaag tgaactgcat aaaatactcc ctgataatct gagtgaatca gttgatgaaa
     5041 acagaaccgc ccacgatggt gatgccatcc ttcgacagcc tgattaccgt cgattccccg
     5101 acctgtagct gaatgctatc cggcgtcagc gcaattttgc tttcctccac ggccagcaaa
     5161 atttccttaa tcgccgtata agcctgtata ccgtcgcctt cgccaccatt gtttacagcg
     5221 tcaacatgca ccgaaccggc aatcgtgcct ctgtcctgaa agacatcctc agataaagac
     5281 cctttcagta ctccggtgat cttgtggcct ttttccaccg taagaaggcg gttaccttcc
     5341 tggatcgtga ccgtctggtc attttttacg gtttcgcggt gatcgtgttt tacggtggtg
     5401 gtacggtcgt tgagcacttc ggtatccatg ttcttctgcg cgtggatgta gacctgttct
     5461 ctccctgtcg catcatcaaa cttcagctca ttaaatccgc tgcccatgta cgttttggag
     5521 cggatggtca tctgcgtctt tgttcccggc aggctgccgg gggggcggtt ttcctggtgg
     5581 taggtgcgcc ccataatgat cggctggtcc ggatcgccgt tgaggaagtc tacaatcact
     5641 tcctgaccca cacgcggtat cgccaggtgg ccaaaaccgg tgcctgccca cgcctgtgcc
     5701 acacggatcc agcacgaact gtcttggtct gccgggttat aacggtccca gttgaacttc
     5761 acccgcacgc gaccatgttc gtcgcagaag atttcctcgc ctgccggtcc tgtcacgaca
     5821 gcgctctgcg ggccgtcgac cagcggtttc agcaacggct gtggtcgcca tgttctgtct
     5881 gccgggatca ccgcaaaatg gttctccagc gccgtccccg ctccctgccg tcctggcacc
     5941 gcctgtggct gttcgccgtg cagttcactt gccaccacct gccattcccg gttcaggttc
     6001 gcctgcggat gccccgtcag cacaattcgt cgtcccggcc atatctccgg cgagcgactc
     6061 attccccgcg cggtttctgc attgtttcgc cagccgtcca tctgccagcg ggcaaagttc
     6121 tgcccgtgag cacccttgaa acgtccgggg tagtcataca cttcatactg cgtgcgctgg
     6181 tagtcctggt gctggccttc ctgttcaaaa cgtccggccc agccggggcg tttaaaggtg
     6241 tagtctttgg tcaccacgga agaagggcgg atttgtgcgc tgtagcggaa ctggctgatg
     6301 cagagggtgc tcacctcggt acgggtgttc gggttccatg ggatttcaaa agattcgggc
     6361 agatggcgga ctgtgtcgca cagcaccagg ctctggtcgg tacttttgta agcatgctcc
     6421 tcataaaaga agatgccttc ctccgccgcc atccggcaca ggaaatcgta atcagtctca
     6481 ccgtactgga cacaaaactc acgggaagga tgcggctcgc tgaacagcgg actccattcg
     6541 gtcaccccgt tttcctgcaa catcgtgccg aggatgcttt tgatgtcctc gttctggaag
     6601 atacggaagt tctggcgcag accggcacgc cacagcggcg ggtgcacctt catgctgtac
     6661 agcatctggt ttttgtcgtt ctccccgagt tcaaaccagg tcaccacgcc tttcacccgg
     6721 cgctgtactt catcgccctg ccatatcgtc aggtaggcca ttttgtccag cacctgcgca
     6781 aattcaaggg agagaaactg ctggctgacc agggagagat cgagggaaaa gagcgaagag
     6841 agtgactggt tcagatgaaa ggaaaccacc gcaaaagcgt ccggcggcag gccgtccact
     6901 tccagtgtga aacgtaatcc ggttgacata gcaacctcca gtaaaatgaa taatgatccg
     6961 taccgtacaa ctgcggataa tgtgataagc gggacgactt cagacgttat tagggaagga
     7021 acagaaaatg ctatggttat gtgagaattt tgtttaaata ttactttaca gaaacgggtg
     7081 tctgttcagt caaaaaaata atcacaagct caacctcttg tttcttggcg ttattca
//
LOCUS       DAZPAC010000210         7122 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZPAC010000210 DAZPAC010000000
VERSION     DAZPAC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.211"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..436
                     /locus_tag="ACHUPM_003604"
     CDS             <1..436
                     /locus_tag="ACHUPM_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR0871281.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQF
                     AVQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            519..1421
                     /locus_tag="ACHUPM_003605"
     CDS             519..1421
                     /locus_tag="ACHUPM_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR0871282.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="ACHUPM_003606"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="ACHUPM_003606"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR0871283.1"
                     /translation="MDVSYLLDSLNDKQRETVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="ACHUPM_003607"
     CDS             complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="ACHUPM_003607"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR0871284.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="ACHUPM_003608"
     CDS             complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="ACHUPM_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR0871285.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5331..6038)
                     /locus_tag="ACHUPM_003609"
     CDS             complement(5331..6038)
                     /locus_tag="ACHUPM_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR0871286.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="ACHUPM_003610"
     CDS             complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="ACHUPM_003610"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR0871287.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6896..7099)
                     /locus_tag="ACHUPM_003611"
     CDS             complement(6896..7099)
                     /locus_tag="ACHUPM_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0871288.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1641 a         1990 c         1853 g         1638 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcgacat cgttcgtctg gatgccttca aaaccagtaa
      121 agagattcag tttgcggtgc agtcagggcc aatgttgatg gaaaacagtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt gatggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagaggaaa ggttaagcat tgtacactcg aggaggccag acaaactggg gctggctaca
      481 cagctgaaaa atcatcagat aaatgaaggg aaacaaaaat gaaaaaaacg ctcgcaacgt
      541 tatttttatt aacgtgtctg ggatcatcat ccgcttatgc ggataacgct ttgattttac
      601 aaactgattt tagcctgaaa gatggtgccg tctcggcgat gaaaggggtc gcattcggtg
      661 ttgaccataa tcttaaaata tttgatttaa cacacgaaat tccaccgtat aacatctggg
      721 aaggcgctta ccgcctgtat cagaccgcca gttactggcc gcaaggttcg gtatttgtca
      781 gtgtggtcga tcctggtgtt ggtacagacc gtaaatcggt agtgctaaaa accaaaaacg
      841 gtcaatactt cgtttcacca gataacggta cgctcacttt ggtggcggaa tcgctgggta
      901 ttgagagcgt ccggcaaatc gatgaaaaaa ccaaccgcct gaaaggttcc gaaaagtcat
      961 ataccttcca cggtcgtgac gtgtatgcct acaccggtgc acgcctggct tccggagcga
     1021 tcacgttcga acaggtgggg ccagagcttc ctgcaaaagt tgtcgaactt tcttatcaga
     1081 aagccaaagc aacaaaaggt gaagtgaagg gcaatatccc gattctcgat attcagtacg
     1141 gcaacgtctg gagcaatatc agcgacgagt tgctgaatca ggcaggaatc aaactcaacg
     1201 acacgttgtg tgtaaccatt tccgaaggtt cccagcaaaa atacgcaggg aaaatgccgt
     1261 atgtcgcaag cttcggcgat gtgccggaag gccagccgat ggtgtatcta aacagcttat
     1321 tgaatgtttc cgtggcgctc aatatggata atttcgcgca gaaacatcaa gttgcgtctg
     1381 gtgctgactg gaatattgat gtgaagaagt gcgctaagta aggtgctgat gcaggatgcg
     1441 gtgtggcgca ccgcatcctg caacggagtt gtaaattaca ccgtctccag ccgggcgtaa
     1501 gccgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg gctatgttca
     1561 ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg cacgcgctgg
     1621 ccgagtttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg atggctaacc
     1681 gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg cagctcgccg
     1741 ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt ttccgcgtag
     1801 gtcagcgtca gtttctgcat cgcacgggtt acgccaacgt aggccagacg gcgttcttct
     1861 tccagacgtc cgccttcatc cagcgacatc tggcttggga acatgccctc ttccataccg
     1921 acgataaaca cctgcgggaa ctccagccct ttcgccgagt gtagcgtcat caactgcacc
     1981 gcatcctgcc aagtatccgc ctgcccttca cctgcttcca gtgccgcatg ggagaggaac
     2041 gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg cgttgccgtc
     2101 accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc ctgctcgtac
     2161 atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag cggcatatcg
     2221 gcagtttctt gcgctaaggc atcgattaat tccataaatc gctgcaaggc gctggcagct
     2281 cgcccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag tgttaactgg
     2341 cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc ccgcgttggc
     2401 gtattcacca cgcgctcaaa cgccgcgtcg tcgttacggt tggcaatcag acgcagatac
     2461 gagagcgcgt ctttgatttc ctggcgttcg aagaagcgca tcccgccgta aatacggtac
     2521 ggcatactgg cctgcaacag tgcttcttcc agtacacgcg actgggcgtt actgcggtag
     2581 agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aagttttgat gcggttaacc
     2641 acaaaacgcg cttcgtcgag ttcgttaaaa gcgcaataga gggaaatagg ctcaccgtcc
     2701 gcgccatcgg tccacagttt tttacccaga cgcccgttgt tgttttcaat cagggcgttc
     2761 gcggcgctca gaatattgct ggtagagcgg tagttctgct ccagacggat agtttcagca
     2821 ccggggaaat cattgaggaa acgctgaata ttctctacct gcgccccgcg ccagccgtag
     2881 attgactggt cgtcatcgcc aacaatcatc actttgccgg tatcgcccgc cagcaggcgg
     2941 atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat gttggtaaaa
     3001 cgttcgcggt agtgttgcag gatatgcggc ttgttaagcc acaactcgtg agcgcgcagc
     3061 agcagctcgg cgaagtccac caggcccgca cgatcgcacg cttcctgata cgcctgatac
     3121 accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg cggacgcagg
     3181 ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca ctgcttctcg
     3241 tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc actgtcgagg
     3301 atctggaaat cctgcggtag attggcgtcc atatggtgcg cacgcagcaa acggtgcgcc
     3361 agcccgtgga aggtgccgac ccacatgccg ccctggctgg tgcccatcag ttgcccgata
     3421 cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat cgagtatggc
     3481 gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg cgtcttacca
     3541 ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgt ttcgcgctgt
     3601 ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc gtaaaaatag
     3661 gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac agatttactg
     3721 attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgcggc agtaaacggc
     3781 tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc tgcattccgc
     3841 tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag atctcgccga
     3901 tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt tttgagcgcc
     3961 catgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac agttccggct
     4021 gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt tttaaggtgt
     4081 cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta atcattgctg
     4141 cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc gcttgttcaa
     4201 tcgaacgaaa acgccaacgc gtcacgtcgt gataaatctc gggttccgct tcccgtaccg
     4261 cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga tgataatttt
     4321 gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta tcgtaaaggg
     4381 tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa aaacgcatta
     4441 tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt gttgaaaatc
     4501 aagatgagta tagatttgcg tggtggagag gttggcatga cccagcagct cctgcacacc
     4561 acgaagatcg ccgctcgact ccagcatatg cgtggcgaac gagtgacgta atttatgcgg
     4621 atgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt tctgcacatt
     4681 acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt cgctaccaaa
     4741 caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac caatcggcag
     4801 gcggcgctct ttgctgcctt tccccattac ccacacttca ccagactcca ggtcgaggtg
     4861 tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca tcacttccag
     4921 cattgcacgg tcgcgtacag cgaggggatc gttgatatca atgtccagca gccgattcat
     4981 atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg aaacaccttt
     5041 cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc tacgtagcgc
     5101 agaaagccgt aacgcgaggc ttgctgctcc cagcccttta cggcgactgc gtacagcaaa
     5161 attgcgcacc atcgtcacat cacattgctg ccagctttgc aggccgtttt cgctggcaaa
     5221 attgatgatc gcctcaagct gacgctggta gttaagcagg gttatcgggc taagctggcg
     5281 ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg tcatacgcgt
     5341 tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag taactgcgtt
     5401 ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacgac acccaaatca
     5461 gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc cggtagcacc
     5521 accagcagct ctggtccgtt aagcggccca agatagtgct gttcctgccc caaacgctga
     5581 atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt cgacggcgca
     5641 cctaagcgcc agcgatccgg aaacaggcgc agactcgcac ctgccaggcc gagatcgcgc
     5701 gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt gagactgcgc
     5761 tgcaggtaga gtaggcgata aaacaggcct tcgttggcga tagcctgttc catcaacagc
     5821 gccatgttct cttccagaac atgaatatga ttacgtgcgc gggccatgtg ccactcgacc
     5881 aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc gcgcgcatta
     5941 cggataaaaa actcaggatt tttaatcaga taatcgacaa ccgcccggtc atcaagctcc
     6001 gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat ccgtcgtaga
     6061 catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag gcgatatcaa
     6121 gacgaccgcc ggggagttcc acgcgtactt cttcggccag caaaccttgc tgaatcccta
     6181 ctgcaaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc ccacgctcat
     6241 aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata ttggcgcgct
     6301 ccggaaaacg ctcgtggctt tccagaacag gaccaagcgt ttctaccgcc gcggtatcga
     6361 catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg cataagattg
     6421 tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga aacggcacgg
     6481 cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca tcggtgacgg
     6541 tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg gtcagtcctt
     6601 tcagacgcac aaaacgggca aagcagcgcg caccgttgcc gcactgcgcc acttcactgc
     6661 catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc ggctcaacca
     6721 ccagcagttg gtcaaaccct acccccaggt gccgatcagc caggcgacga atcagctccg
     6781 gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg ccaaggccat
     6841 gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact gacttttagt
     6901 aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc accgtggatt
     6961 gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca ggcgggaaat
     7021 agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat acagtgagtg
     7081 ccttaaacac gtttttcatt atggattgcc tgtaaagttc at
//
LOCUS       DAZPAC010000211         7117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZPAC010000211 DAZPAC010000000
VERSION     DAZPAC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.212"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..505
                     /locus_tag="ACHUPM_003612"
     CDS             <1..505
                     /locus_tag="ACHUPM_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR0871289.1"
                     /translation="VFSFKDTLLMRMNNLNNDLVNYSHDNTRTSVGARLAMYEVGLKT
                     YSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDLIDTLSTRGIPGVVLTILA
                     FSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTIILLCAYFKAQS
                     DQCLLDK"
     gene            complement(575..1546)
                     /gene="rfaC"
                     /locus_tag="ACHUPM_003613"
     CDS             complement(575..1546)
                     /gene="rfaC"
                     /locus_tag="ACHUPM_003613"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR0871290.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(1550..2596)
                     /gene="rfaF"
                     /locus_tag="ACHUPM_003614"
     CDS             complement(1550..2596)
                     /gene="rfaF"
                     /locus_tag="ACHUPM_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR0871291.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(2606..3538)
                     /gene="rfaD"
                     /locus_tag="ACHUPM_003615"
     CDS             complement(2606..3538)
                     /gene="rfaD"
                     /locus_tag="ACHUPM_003615"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR0871292.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            3752..4948
                     /gene="kbl"
                     /locus_tag="ACHUPM_003616"
     CDS             3752..4948
                     /gene="kbl"
                     /locus_tag="ACHUPM_003616"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR0871293.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            4958..5983
                     /gene="tdh"
                     /locus_tag="ACHUPM_003617"
     CDS             4958..5983
                     /gene="tdh"
                     /locus_tag="ACHUPM_003617"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR0871294.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            6222..>7117
                     /gene="waaH"
                     /locus_tag="ACHUPM_003618"
     CDS             6222..>7117
                     /gene="waaH"
                     /locus_tag="ACHUPM_003618"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFR0871295.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         1746 a         1729 c         1791 g         1851 t
ORIGIN      
        1 tgtattttct tttaaagata cattgcttat gagaatgaat aacttaaata acgatttagt
       61 taattattcg catgataaca ccagaacttc agtcggtgcc cgtctggcaa tgtatgaagt
      121 tggcttaaaa acatattctc caataggaca atcactggaa aaacgagcgg aaaaaataca
      181 tgaactagaa gaaaaagagc ctagattgag tggcgcttta ccctatatag attctcattt
      241 gcataacgat ctcatagata cgttatcaac gcgtggtatt cctggagttg tattaacaat
      301 tttagccttt tcagcaatac tcatatatgc cttaagaact gctaaagaac cttatatttt
      361 aatcttgctt ttttcactac tggtagtagg cctaagtgat gtaatactct tttctaaacc
      421 ggttccgact gctgtgtttg tcaccataat attgctttgt gcttatttta aagcacaatc
      481 agaccaatgt ttattagata aataatgaat atgtgaaata aaatcagcaa ggaggctgat
      541 tttatttcaa aattatctat taaggggttg taatttaaat catagcaacg ttttcttcaa
      601 tgaatcgctt aacagcattt gctgtcaatt gagacaactc attccccgga gccctacaaa
      661 ccatctgatt cttcccatat ccaccaatta atcccggatc ggttggtcca taaaccgtga
      721 tattgggtct atccagtgcc gccgttaaat ggcttaaccc cgtatccacc gacactacaa
      781 atttagcccc ggccagcacg cgggcaacgc cttccagact catcttcggc aatacttcaa
      841 cataagcaaa tccttccgcc agtcgtttcg cccgctcttc ctcatgcggc gcgccccacg
      901 gaagtttaat ccgtattcct gaatcagcca gtaaaccaat caattctcgc cagtgttctt
      961 ccggccagtg tttatcatca cgggtcgtcg catgaagaaa tacggcatat tcgccagcat
     1021 ctgtaggcag gttcgtcagg aaatgctgtg caatagcata atcgccctgg gtttgcggtt
     1081 tgctatagcc cagacttttg gcaaacagtt cgcgggtgcg ttctacggcg tgctgctgtt
     1141 ttgcaatatg atgcttacga ttgtaaaaca gactggctaa aggttcgcga gcggtttgcc
     1201 agtccatgcc atgctttacg ccatgcgcca gacgcgtcac cagcgccgcg ctttttacca
     1261 gcccctgagc gtcgataact gcgtcatagt tctctgcttg tagcgcttca cgaaacgctt
     1321 tgcgttccgc ttttatgggg gccgagaacc aggctttacg ccagcgacgt attgccacag
     1381 gaataactcg ctcaacggcg gcgtgccagg aaggaatctg tgcgaaccct tcttccacca
     1441 cccagtcaaa cttaatccct gggattgcct gctgggcatc agtgagtgcg ggcaacgtat
     1501 ggagaacatc gcccatcgac gatgttttaa cgatcaaaac ccgcatccgt caggcttcct
     1561 cttgtaacaa tagcgcgttg agttcttcca gtacgcgctg gggagtaatg tcgattaagc
     1621 tctggtgata accctccgca gcgtcacctt tacgcacttt gtgatagccg gtaatcaggc
     1681 ggatcacgcg cgctttatgg gatagcggcg gtgtgaagtc cgggctactc ggaccataca
     1741 gggcaaccag cggacgattg agcgccgccg caacgtgcat caggccagaa tcgttagtga
     1801 caatggcttt acaggctgca atcaggataa ccgcttgatc aagctgtgtt tcccccgcca
     1861 ggttccgaca ccatgcctgc tgctcggtat tcaaagcggc aagaatctca ttgcccgctt
     1921 catgatcttt cgccgagcca aacagaacca cctgataacc ttcatcaatc agctgctttg
     1981 ccagttccgc atagtggtag tgtggccagc gttttgccgg accaaactcc gcacccgggc
     2041 aaaaaccaat catcggacgt tctgatgaaa gcgaaaattg attacaggta tatgattttt
     2101 caccttcgct cacctgcaat tgcggccata acaacggttt tggtagatct tgtgcggtac
     2161 gcatcacgcc tttgtcatag gccagcgcga catagcgttc caccattagc ggccaggctt
     2221 ctttatcgag cacgcgtaaa tcgttgagta aaccgtagcg catctcgccg cgccagccgg
     2281 tgcgatgagg aatacccgcg aagaaaggca ctaatgcaga tttgaaggag ttgggtaaga
     2341 cgtaggcgcg gtcgtagcgc ttttcacgca ggctatgacc cagtttgcgg cgttcgccga
     2401 tttccagcgc tccgtgaccg agaggcatag gaatagcttc gttaacttcc ggcatccgcg
     2461 ataataatgg acggcaccat gccggtgcca tcacatcgat tatcgcctgg ggatagcgcg
     2521 cctggagcgt gcgatagaga ctttgcgaca tcatcatgtc gccaacccaa gacgggccga
     2581 tcaccagtat tttcatgcag agctcttatg cgtcgcgatt cagccaggcc atgtattccg
     2641 ttacaccttc agcaacggtt ttgaacggtt tgtcgtaacc cgccgcgcgc agatttgtca
     2701 gatctgcctg agtgaacgcc tggtagcggc ctttcagctt atccgggaac ggaatgtatt
     2761 cgatctggcc tttcttgtga taagccagcg tagcatcagc tacagcctgg aaggattccg
     2821 cacgaccagt accgaggttg aagatgccgg aaacgccatt ttccaggaac cacagattca
     2881 catcagccac gtcgcccaca tagacgaagt cgcgtttgaa gttctcgcta ccttcaaaca
     2941 gcttcggtga ttcaccgttg ttaagctggg tgttgagatg gaaagcgacg ctcgccatgc
     3001 tgcctttatg gccttcacgc ggtccataaa cgttgaaata gcggaagcca acaatctgcg
     3061 agttagcttc aggcaggatt tggcgaacat attcatcaaa caggaatttt gagtaaccgt
     3121 agacgttcaa cggtttttcg tactcgcggg attcaataaa gtcggaggtg cgtccgccgt
     3181 aggtggctgc ggaagaggca tacaggaacg ggatttcgcg ctccagacag tagtgcagca
     3241 gctctttgga gtattgatag ttgttatcca tcatatactt gccatcccac tcggtggtgg
     3301 aagagcacgc gccttcgtgg aaaatcgctt cgacatcgcc gaactcttcg ccagccataa
     3361 tctggatcag gaagtcttcc ttatccatat agtccgcgat atccagatcc accaggttca
     3421 caaacttggt gccgtctttc aggttgtcca ccaccagaat atcggtgatg cctttatcat
     3481 tcagggcctt aacgatgttg ctgccgataa agcccgcgcc gccggtaacg atgatcataa
     3541 ctgtaacctt cgaattatgg agtcagagac aatctcagac acgaatactt ctatcatatc
     3601 actacacggc gttggcttca gccattcgcc aaaagtgaga ttgatggacg ttatttatcc
     3661 tgaattttgc agaagtgtta acgcgtcatc tcgtcgcgac ctataagttt gggtaatatg
     3721 tgctggaatt tgccctgtct ggagaatcgc aatgcgtgga gaattttatc agcagttaac
     3781 caacgatctg gaaaccgcac gggcggaagg gttgtttaaa gaagagcgca ttattacgtc
     3841 tgcgcagcaa gcagatatca ctgtggctga tggaagccac gtcattaact tttgtgccaa
     3901 taactatctc gggctggcga attatccgga tctgattgcg gcggcaaagg cgggaatgga
     3961 ttctcacggt ttcggcatgg cttcggtgcg ttttatttgc ggcactcagg acagccataa
     4021 agagcttgaa caaaaactgg cggcattcct ggggatggaa gatgcgattc tctactcttc
     4081 ctgctttgat gctaacggtg gcctgtttga aacgctgctg ggcgcggaag acgccattat
     4141 ctccgacgca ctgaaccacg cgtctattat tgatggtgtg cgtctgtgta aagctaaacg
     4201 ctatcgctat gccaacaacg atatgcagga gctggaagca cgtctgaaag aagcgcgtga
     4261 agccggtgcg cgtcatgtgc tgattgccac cgatggtgtg ttctcgatgg acggcgtgat
     4321 tgccaacctg aagggcgttt gcgatctcgc agataaatat gatgccctgg tgatggtaga
     4381 cgactcccac gcggtcggtt ttgtcggtga aaatggtcgt ggttcccatg aatactgcga
     4441 tgtgatgggc cgggtcgata ttatcaccgg cacgctcggt aaagcgctgg gcggggcttc
     4501 tggtggttat accgcggcgc gcaaagaagt ggttgagtgg ctgcgccagc gttctcgtcc
     4561 gtacctgttt tccaactcgc tggcaccggc cattgttgcc gcatccatca aagtactgga
     4621 gatggtcgaa gcgggtagcg agctgcgtga ccgtctgtgg gcgaacgcgc gtcagttccg
     4681 tgagcaaatg tcggcggcgg gctttaccct ggcgggagcc gatcacgcca ttattccggt
     4741 catgcttggt gatgcggtag tggcgcagaa atttgcccgt gagctgcaaa aagagggcat
     4801 ttacgtcacc ggtttcttct atccggtcgt tccgaaaggt caggcgcgta ttcgtaccca
     4861 gatgtctgcg gcgcataccc ctgagcaaat tacgcgtgca gtagaagcat tcacgcgtat
     4921 tggtaaacaa ctgggcgtta ttgcctgagg atgtgagatg aaagcgttat ccaaactgaa
     4981 agcggaagag ggcatctgga tgaccgacgt tcctgtaccg gaactcgggc ataacgatct
     5041 gctgattaaa atccgtaaaa cagccatctg cgggactgac gttcacatct ataactggga
     5101 tgagtggtcg caaaaaacca tcccggtgcc gatggtcgtg ggccatgaat atgtcggtga
     5161 agtggtaggt attggtcagg aagtgaaggg cttcaagatt ggcgatcgcg tttctggcga
     5221 aggccatatc acctgcggtc actgtcgcaa ctgtcgtggc ggtcgtaccc atctgtgccg
     5281 caacacgatc ggcgtcggcg ttaaccgccc gggctgcttc gccgaatatc tggtgattcc
     5341 ggcgttcaac gccttcaaaa ttcccgacaa tatctccgac gacctggctt ccatttttga
     5401 tcccttcggt aacgccgtgc ataccgcgct gtcgttcgat ctggttggcg aggatgtgct
     5461 ggtctctggt gcaggcccga ttggtattat ggcagcggcg gtggcgaaac acgttggtgc
     5521 acgcaatgtg gtgatcactg atgttaacga atatcgtctc gaactggcgc gcaaaatggg
     5581 tatcacccgt gcggtcaacg tcgctaaaga aaaccttaat gatgtgatgg cagaactggg
     5641 catggccgaa ggctttgata tcggtctgga aatgtccggt gcgccgccag cgtttcgtac
     5701 catgctcgac accatgaacc acggcggtcg cattgcgatg ctgggtattc caccgtccga
     5761 tatgtctatc gactggacca aagtaatctt taaaggcttg ttcattaaag gtatttacgg
     5821 tcgtgagatg tttgaaacct ggtacaagat ggcggcgctg attcagtcag gtctggatct
     5881 ctcgccgatc attacccatc gtttctctat cgatgatttc cagaagggct ttgacgctat
     5941 gcgttcgggc cagtccggga aagtaattct gagctgggat taacacgaac aagggctggt
     6001 attccagccc ttttgcctga ggataatccg ttaaatatgt aaaatcctgt cagtgtaata
     6061 aagatatcgt aattgcgctg atctcttata tagctgctct cattatctct ctaccctgaa
     6121 gtgactctct cacctgtaaa aataatatct cacaggctta atagtttctt aatacaaagc
     6181 ctgtaaaacg tcaggataac ttcagaggtc gtcggtaatt tatgatgaac agcaccaata
     6241 aacttagtgt tattattccg ttatataatg cgggcgatga tttccgcact tgtatggaat
     6301 ctttaattac gcaaacctgg actgctctgg aaatcattat tattaacgat ggttcaacgg
     6361 ataattctgt tgaaatagca aagcattacg cagaaaacta tccgcacgtt cgtttgttgc
     6421 atcaggcgaa tgctggcgca tcggtggcgc gtaatcgtgg gattgaagtg gcaacgggca
     6481 aatatgtcgc ttttgtcgat gctgacgatg aagtctatcc caccatgtac gaaacgctga
     6541 tgaccatggc gttagaggac gacctcgacg tggcgcagtg caacgctgac tggtgttttc
     6601 gtgaaacggg agaaacctgg aaatccatcc ccagcgatcg ccttcgctca accggcgtat
     6661 taaccggccc ggactggctg cggatggggc tttcttcgcg ccgttggact cacgttgtct
     6721 ggatgggggt ttatcgccgc gatgttattg ttaaaaataa cattaaattt attgccggat
     6781 tacatcatca ggatattgtc tggacaacag aatttatgtt taatgcgctg cgtgcgcgat
     6841 ataccgagca atcgttatat aaatattatc tgcataatac gtcagtgagt cggttgcata
     6901 gacaagggaa caaaaacctt aattatcaac gtcactatat taagattact cgtctgctag
     6961 agaaattaaa tcgaaattat gccgacaaaa ttacgattta tccggaattt catcagcaaa
     7021 taacttacga agcattgcgt gtttgccatg cggtgcgcaa agagccggat attattaccc
     7081 gccaacggat gattgccgag atatttactt ccggtat
//
LOCUS       DAZPAC010000212         6987 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZPAC010000212 DAZPAC010000000
VERSION     DAZPAC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.213"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(203..625)
                     /locus_tag="ACHUPM_003619"
     CDS             complement(203..625)
                     /locus_tag="ACHUPM_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFR0871296.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRST
                     TAFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
     gene            747..1025
                     /locus_tag="ACHUPM_003620"
     CDS             747..1025
                     /locus_tag="ACHUPM_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871297.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKIFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
     gene            complement(1017..1307)
                     /locus_tag="ACHUPM_003621"
     CDS             complement(1017..1307)
                     /locus_tag="ACHUPM_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871298.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
     gene            1547..3193
                     /gene="cka"
                     /locus_tag="ACHUPM_003622"
     CDS             1547..3193
                     /gene="cka"
                     /locus_tag="ACHUPM_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="HFR0871299.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGH
                     GNNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNP
                     FGGVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINE
                     KQKQKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKL
                     NSYKNTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEVKAELA
                     KAESDVQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAV
                     ENELKNAVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEK
                     YAKIAQDLAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDEL
                     SKNLTKISKSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVAL
                     MFSFIVGVPLGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
     gene            complement(3190..3480)
                     /gene="cki"
                     /locus_tag="ACHUPM_003623"
     CDS             complement(3190..3480)
                     /gene="cki"
                     /locus_tag="ACHUPM_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="HFR0871300.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLY
                     PYSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
     gene            3510..3641
                     /locus_tag="ACHUPM_003624"
     CDS             3510..3641
                     /locus_tag="ACHUPM_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFR0871301.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
     gene            complement(3836..3979)
                     /locus_tag="ACHUPM_003625"
     CDS             complement(3836..3979)
                     /locus_tag="ACHUPM_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0871302.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDT
                     QNS"
     gene            complement(4646..4837)
                     /locus_tag="ACHUPM_003626"
     CDS             complement(4646..4837)
                     /locus_tag="ACHUPM_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR0871303.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            4905..5228
                     /locus_tag="ACHUPM_003627"
     CDS             4905..5228
                     /locus_tag="ACHUPM_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR0871304.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            5437..5871
                     /locus_tag="ACHUPM_003628"
                     /pseudo
     CDS             5437..5871
                     /locus_tag="ACHUPM_003628"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5906..6466
                     /locus_tag="ACHUPM_003629"
     CDS             5906..6466
                     /locus_tag="ACHUPM_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR0871305.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYLEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            6473..6706
                     /locus_tag="ACHUPM_003630"
     CDS             6473..6706
                     /locus_tag="ACHUPM_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR0871306.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
BASE COUNT         1877 a         1385 c         1941 g         1784 t
ORIGIN      
        1 aaaaaaaaca gtgtcatgag gtttacgcag gcaaaaccag cgttattcac atggctgaat
       61 agctatgcat acggttaaaa tttatcaggt gcgatcgcgg cagcttttcg ggtggtttgt
      121 tgccattttt acctgtcagc agccgtgatc gcgctgaacg catttcagcg gtgcgtacaa
      181 ttaagggatt atggtaaatc ccttactgtc tgccctcgta tccatcgaga taaaccgcag
      241 aaatggtgtc tgccattagt ttcatttttt tcggcgaagg catatcgctg tttgtgtatt
      301 ttctgccagc aaaggctgtt gtgctccgga gtgagtgaaa aaatttttct gagtggaatt
      361 cttttacacg ttgctgtgcc gtttctgctg atactccggc tgctttgtct ttcatcccca
      421 tatcccggaa ctgctgaaag aacgggagca tcatacgtat ggtgtctgct ttctgggtgt
      481 agaagttagg atcaaatcca tcatctgaag cataaacata atgccgggta tgtcgttcca
      541 ggctggtctt tggtgtgcat cctgtaagag ctattgttgc tgttatcaga ggtatcagaa
      601 gcagcctggc agcggcagat ctcataaaca tctccttgcg tttttaatct tgatactcag
      661 ggagttatat ctccgaagag tatctcttat catacgtcgg tttactccca atgtgatttg
      721 atggtcaatg agtcaggaat aatcccgtga aaaaatgtgt gccagtcctg ctttttagtg
      781 ttctttctgc ttcggttgcc agtcatgcgg acagcaatgt ttttcaacaa aaggccgaga
      841 agacaaaaaa cggcattgtt gcggatgtta atgccagaca gaaaatattc agtaacagac
      901 tgaagataca acttcagatc gatgagttga gagcagagct ggctggcacc agagatgccc
      961 aaaaaagaga ggatttgcgt aaaaaactgg agtctctgga gcggcaaaag aaatccttac
     1021 gctgatgggg gcgtattttc cccggtttct tccataacct ttttaccgtt cagatattta
     1081 cgggcgaggc gaatgcgtct tgcctggtat accgccgagt aagcctgggt gggattcaca
     1141 ccattactga tttcactgac cacatgacgc tgatcagcca gtgtcagcgt gtaaacctgt
     1201 ttctggatga ggtcacgggc tttgtcatgc tcatatccgg cagcaacaag ggtgtcgaga
     1261 agtcgtttgt ctgttttgtt cagtttatgg cggggtgatt tgctcacatc gttctccgtt
     1321 ttttcatgat ccaacagttt tatatctcat cctgatttgc tccggtgttc tgtttcgacg
     1381 ttctcaatgc ctgcctcccc atagtgtgat ccttattgca aaaatgaaaa tccatgctct
     1441 tgacatggac aatactgagt agtaggtttt actgtacata aaaccagtgg ttatatgtac
     1501 agtatattgt ttttaattta ttgttttaat tatcaaagag gaatttatgg ctaaagaact
     1561 aagtggatat ggaccaactg ctggtgagtc tatgggggga acaggggcga atctgaatca
     1621 gcaaggtgga aataataata gtaatagtgg ggttcactgg ggtggtggct ctgggcatgg
     1681 taataatggt ggtcagggaa attcaaattc atctggttct acatcaactg tgatgaaaac
     1741 cggtgagtca tatctgactc cgtggggtga tgttgtcatt aataatgatg gccttccggt
     1801 gatgaatggt attgttatga cagaagagaa ttcaactctg gttgataatc catttggagg
     1861 ggtctccaga gttcttaatt ctttaattag tgatatgcca tcactttttg cagaaagctc
     1921 aggaaataac aacaataaca ctgcttcagt taatactgca ccaacgaatg ctcaggtcag
     1981 tgatatggat aagagttcga aggtggtggg taatgtaatt aatgaaaaac aaaagcagaa
     2041 gaataaaatt gctacacaga ttagtgaaaa acagaagaaa atcgaggaga tgaaaaaagt
     2101 attcaagcat cattcttatc atggtatcac tgatcttgag agggatgttg atgagttgca
     2161 aaaaaaatca aatcagcttg atgcagatat tagcaaactt aattcttata aaaatacatt
     2221 gcaaagtaaa attggtgatg tgaataaaca gaaggaagct gaagaaaagg ctcgtgaaaa
     2281 tgcagaggtc gctgaacatg aaactcttaa tgaagagaaa caggcggtgg cggaagcaga
     2341 gaagcgttta gctgaggtga aagctgaact ggcgaaggct gaaagtgatg tacagagtaa
     2401 gcaagctact gtttccagag ttgcaggaga gcttgaaaac gctcagaaga gtgttgacgt
     2461 gaaggtcacc ggatttccgg gatggcgtga cgttcagaaa aaactacagc gtcagcttga
     2521 agctaagcag gctgagtact ccgcagttga aaatgaactt aagaatgctg ttagtttcag
     2581 ggatggtaag gccgctgagg ttaaagaggc agagcagaaa ttaaaagaag ctcaggatgc
     2641 tcttgaaaag agccagataa aagatgctgt tgatactatg gttggatttt atcaatatat
     2701 aaccgaacaa tatggagaaa aatacgcaaa aattgctcag gatttagcgg aaaagtctaa
     2761 aggaaagaaa attcaaggtg ttgatgaagc tctggcagca tttgaaaagt ataagaatgt
     2821 actggataag aaattcagta aggttgatcg ggatgctatt tttaatgcgt tagagtctgt
     2881 taattatgat gagttatcta agaatttaac gaagatatca aagtcgctta aaatcaccag
     2941 tcgagtatct tttctatatg atgttggttc tgattttaaa aatgcaatag aaactggtaa
     3001 ctggcgacct ttgtttgtca cgctggagaa aagtgcggtc gatgtaggtg tggcaaaaat
     3061 tgtggcatta atgtttagtt ttattgttgg tgtgcctctt ggtttctggg ggattgcaat
     3121 tgtcacaggt attgtttctt cttacatagg ggatgatgag ttgagcaaac ttaatgagtt
     3181 gttagggatt taatttctct ttagagtgta atatatgaaa cttggcaatc ctactgggat
     3241 tgccattaat aaataaaaca atgcagataa cttgcataag taccagggtt ctttttttaa
     3301 ctttgtattt tctttgatat atctagatat ggttaattta gagtatggat atagcaatac
     3361 atgtattgat gatataaata taaataacaa aggagtgtta tcattgtcgt taaatattaa
     3421 aataagaatc catggtataa gtgattcagg taaattatgt aggtagtatt ttaaatgcat
     3481 aagtattttt acttttgttg gaaagaaata tgaaaataat aatttttcta attacaatat
     3541 tattcttgtc aggatgtcaa gcaaactata ttcgtgatgt acaaggagga accgttgcac
     3601 catcttcgtc ttctgaactg acggggatcg cggttcagta gaaaagatca aaggatcttc
     3661 ttgagatcct ttttttctgc gcgtaatctg ctgcctgtaa acgaaaaaac cgcctgggga
     3721 ggcggtttag tcgaaggtta agtcagttgg ggactgctta accgggtaac tggctttagt
     3781 ggagcacaga taccaaatgc tgtccttcca gtgtagccgc tataaggctt caacttcaag
     3841 agttctgcgt atctaaatcc actatccagt taccaatggc tgctgccagt ggcgttaagt
     3901 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3961 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4021 accaacagcg tgagctatga gaaagcgcca cgcttcccaa agggagaaag gcggacaggt
     4081 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagccacca gggggaaacg
     4141 cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc ggtttttgtg
     4201 atgctcgtca ggggggcgga gcctatggaa aaacgccagc tgcgtggcct tttccgggtt
     4261 cttgtttttt gctcacatgt ttcttttggt gttatcccct gattctgtgg ataacagcat
     4321 ttcgtttttg agtgagtaga taccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca
     4381 gtgagcgagg aagcggaaaa gcggctggac gtgcattttc tccttacgca tctgtgcggc
     4441 atttcacacc cggtatggcg cacttttcat accatccgca ctgatgccgc atggttaagc
     4501 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgctaa
     4561 aacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgctcac aggccagctg
     4621 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatccccg aagcgtgcga
     4681 ggcagctgcg gtaaagctcg tcagcgtggt cgtgaagcgc ttcgcagatg tcagcctggt
     4741 cgtcggcgtc cagttcattc agtttttcca gcagggtcag cgtctggctt ctgatgaagc
     4801 gggccatgtt aagggcggtt ttttcctgtt tagtcacagt tctgtttctc catgtgtggg
     4861 gagtctgtcg gaggttttta atgataccgg tgaaacgcga aaggatgctc acgatacggg
     4921 tcacggatga ggaacatgcg cggctgctgg cacgttgtga gggaaaacaa cttgcgtcat
     4981 ggatgcgtaa ggtctgcctg ggggcaccgc catcgaaaac gtcaggtctt ccgacactgg
     5041 caccgccgct gttgcgccag ttcgcttcgg tcggcaataa cctgaatcag atagcccgga
     5101 agataaacag cggacagtgg tcggggcatg accgggttca tgtggtggct gcactgatgg
     5161 ccatcggacg tgaactgagt gagctgaggg atgaggtccg taaacaggga gaacgggatg
     5221 atagttaagt ttcatgccag gggaaaaggc ggtggcagtg gtccggttga ttacctgctg
     5281 ggccgggagc gtaaccggga aggggcgaga gttctgcggg gtgctcccga agaggtgcgg
     5341 gaactcatcg atgccacgcc gtttgcgaaa aaatacacct cgggggttct gtcttttgca
     5401 gagcagaccc tgccaccggg agagcgtgaa agggtgatgg agagctttga gcgggtactg
     5461 atgcccggtc ttgaaaaaaa tcagtacagc atcctgtggg tggagcacca ggacaagggg
     5521 cgtcttgagc tgaattttgt catcccgaat atggagctgg cgagcggaaa acggctccag
     5581 ccctattacg accgagcgga ccgtccgcga atcaatgcct ggcagacact ggtgaatcac
     5641 cattacgggc tgcacgaccc gaacgcaccg gagaacaggc ggacacttgt caccccgaat
     5701 aatctgccga aagcgaaaca ggaggccgca gaagcgatta cgaggggact ggaggctctt
     5761 taccatgccg gagcgctaaa aacgcgtcag gatgtcactg aggcgcttac agcggcaggt
     5821 tttgaggttg tgaggacaac caggagcagt atcagtatcg ccgacccgga gggtgggcgt
     5881 aatctcaggc tgaaaggggc gctgtatgag caatctttta caaatgggga cagacttcga
     5941 gaagaaactg aaagagcgtg ccgtctctac agagaacatg ctgaacagcg agttcaggaa
     6001 gctcgaagcg tctgtagaca gggaactgaa cttaaacggg cagaaaatca gagacgctat
     6061 cagcgcacac acagcagcgg tgaaagaaca actggagacg ctgaacagtg ccgtgaacgc
     6121 gcagttcagc gccacagaga acgaactggc ccggcggcag gaagagctgc tctggaagct
     6181 ggtcaaaggg cggatactgt acccgtcgct gacggcgctt tgcgtcacgg gcgggatttt
     6241 tctggcgtca tgggggctga ttcagtggca ggaaagccgg attgcggcga acatcctggc
     6301 tatcagggac caggaagaga cgctggcgaa actccgggaa aaaacatggg gtgtgacgta
     6361 tctcgagggc aggaacggga aatttctggt gttgccgtcc ggggtgaagg gggaaaacaa
     6421 ctggacggtg gagaagaaga acgcagtaag actggtcagg gagtaagtga gtatgacaga
     6481 gctggaaatg cacttactga acgccttaga gcaattgcag caggactata cgcagcggct
     6541 gaaagactgg gagagcgcct tcacggattt gcagcatatg ttcgagctga cacgacagga
     6601 gaacgggcaa ctgagggaac agtgcgacga cttgagtcag caggtgcaga gattagccgg
     6661 gcaggtggac cacttgagtc ggttatttat cacgaacagc agataaggga tgaacaggaa
     6721 aggcaggcac gcctggaacg ggagcgtgcg cggtcctggc atgaagagca ggaacgggaa
     6781 gcacgggtat ggcgtggccc gtcaataggg atgtgacggt gagttgttcc gggaggttgc
     6841 cagatggtac cggaacgcgt taaaaacgca tccgggcgcg attctggcgt gtcgtttttg
     6901 ttgctgtgtt ttgcctcttt gccagatcgc gctcattcct cagcctcatc ccgcgatgtc
     6961 tgcgtatgtc atcaactgaa aagatgg
//
LOCUS       DAZPAC010000213         6946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZPAC010000213 DAZPAC010000000
VERSION     DAZPAC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.214"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..100
                     /locus_tag="ACHUPM_003631"
     CDS             <1..100
                     /locus_tag="ACHUPM_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine utilization protein EutE"
                     /protein_id="HFR0871307.1"
                     /translation="RGYDYPVDFPRLIQEKRKEWEQIAGMPVAEVF"
     gene            complement(172..381)
                     /locus_tag="ACHUPM_003632"
     CDS             complement(172..381)
                     /locus_tag="ACHUPM_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFR0871308.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
     gene            773..949
                     /locus_tag="ACHUPM_003633"
     CDS             773..949
                     /locus_tag="ACHUPM_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871309.1"
                     /translation="MNLVDAFVKKVISGPYKEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            complement(1014..1109)
                     /gene="dqlB"
                     /locus_tag="ACHUPM_003634"
     CDS             complement(1014..1109)
                     /gene="dqlB"
                     /locus_tag="ACHUPM_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFR0871310.1"
                     /translation="MINLLIIVLRAVVAVANALIAVLELIRELID"
     gene            1610..1861
                     /locus_tag="ACHUPM_003635"
     CDS             1610..1861
                     /locus_tag="ACHUPM_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871311.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            complement(1933..2085)
                     /locus_tag="ACHUPM_003636"
     CDS             complement(1933..2085)
                     /locus_tag="ACHUPM_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0871312.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            2377..3585
                     /gene="trbA"
                     /locus_tag="ACHUPM_003637"
     CDS             2377..3585
                     /gene="trbA"
                     /locus_tag="ACHUPM_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFR0871313.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            3604..4674
                     /gene="trbB"
                     /locus_tag="ACHUPM_003638"
     CDS             3604..4674
                     /gene="trbB"
                     /locus_tag="ACHUPM_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HFR0871314.1"
                     /translation="MTIEYFATRIAKHISVQAIWPNGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            4667..>6946
                     /gene="trbC"
                     /locus_tag="ACHUPM_003639"
     CDS             4667..>6946
                     /gene="trbC"
                     /locus_tag="ACHUPM_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFR0871315.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWD"
BASE COUNT         1911 a         1750 c         1631 g         1654 t
ORIGIN      
        1 aagaggatat gattaccctg tcgactttcc tcgcctaatt caggagaagc gtaaggagtg
       61 ggagcagatt gcaggcatgc ctgtagccga agtattttaa gataagctcc ctccataaca
      121 gccggttcaa atgagccggc ttttgttcat tacaacagat aacttatgcc gttaccgata
      181 aattcgacca agtgctttaa tcgcagcaat ttttatttcc atatcatcac tttgactcaa
      241 tctatgtaat tcacgagtaa tgttctccgc ctgacaccgg ccttcaccta atgcatagat
      301 cgcagcaagt gtaacgtctt ttgatttact cttcatcatt tcaaacaaga aagtattagc
      361 tatcgcagga ggcatgctca taatcaatcc tcttcatgaa ataaacaatg gcactcactt
      421 acaaacagga gtccactatc gcaagcgact caacttagca atatctctca atttgaatga
      481 aaaaaacaga tggcatgtga cagggaaccg tccgcatgat gtccgtgacc atttaacccc
      541 ataagctaac cttgtcacaa acgggcgcag aacagcctgc cagacagaca acaaaacaaa
      601 agtccaagat aaaacatgaa taccggtatc tcaatgcgaa gaaataatct ccgtagaaac
      661 ccccatgaga aaaatatcat atgggagaaa atcgcaatta tcacagccag cgcattcatt
      721 attttcgctg tgctctggca ttaattattt tcaacacaaa aaggaaaaag caatgaatct
      781 ggtagacgcc tttgtcaaaa aagtaatatc cgggccatat aaagaatatg gaaaatggtg
      841 gatagatgtt gaatacattt catggggcgt tcccgggaaa acaagactga tgtttgagtc
      901 aaaggagcag gcactggaag ttaaagaagg gtacaaattt ttaacgtgag atctcttttt
      961 gttaaagggc aggaagcggt aactccctgc ccttagttac gtttccaggc ttatcagtcg
     1021 ataagttccc ggatcagttc cagaacagca atcagcgcgt ttgcaacggc aactactgcc
     1081 ctgagaacga tgattaacag attaatcatc cgcttctcca tcactggaga aagaaccccg
     1141 ttgttcgctc ttacactgcc tgtcacaacg gtttctatag tgtcagttat agacagttct
     1201 tctccgacca gagcaccaaa accagcacca cctgatgttt tggccaggac ttgtaacctg
     1261 ttcttcccgc tgaacaatat gccggacaga acatgcctca gagctgacat gccgagtata
     1321 cacttatcca gtacttgcag aaatataatt ccagacgtac tatctacagg tcagttatag
     1381 acagttcttt tccgaccaga gccaccaact ctgtagcgga acagtaaaaa agccgacctc
     1441 accagtcggt ttttttacgt ctgttgtttc tcaaaatcct cccgccatgc ccgtcacacc
     1501 ggagaaactc atgctctgaa tcagaaccaa ccatctcaac tttgccgtct cctgaaaaaa
     1561 ctggcacatt cagttcgata actacccata aatacagaga gaaaatccga tgtcagaaaa
     1621 gtggagtaaa gaattcgact cttgctttca ggtggggagc atagaagaaa ttgccgatgc
     1681 tctcgagaag cgcagcggaa gcgaggaaaa cgcgttagcc atgctggcaa tgacagcctt
     1741 cacactcatg acccgacgtg gtatctgcga aatgcagaac gtggcccccg atggcaaagg
     1801 cattcggctg gagctaatcg gcttcaggga aaatgaaaca ataccggata cgcttcattg
     1861 aagaagaaag ggcgcaatga ggtcagcctt cgcaacaaag cggcggcaga aaacctgccg
     1921 ccgtgttgcc cgttaacgtt taacttcgta ggctaacgtt gccacaagca ctgtgtttcc
     1981 ctgctggagc cggagtccgc aaagcgaatc cctcaccatc cagacaaaac acagaatcgt
     2041 cacacagatg acgattaaca tcattaaaaa cgttcgctgt ggcatcatag cccctccatt
     2101 gccttacggc ggttatcggg ctactataat gttgtcgaag cattacagta gagcccttgg
     2161 ttgattgata aaaaaatctc caggggcttt ctgctgtata ccgtccacaa aacgctttcg
     2221 gcatacagcc tcaagcgccc gcagctattc taacgacttt cgtatctgca acaatccttt
     2281 tatcgcacgg ctaattcctc tggttagctc ttatcgcctg caaataaatt tttaaccttt
     2341 ccgtttgatt taaattccag aaatggagac tttgtcatgt cttacaatcg acagcctgtc
     2401 gcagaagatc ctatgcagat atggggcgct gttggtgtcc ttcttatcct gctgctattc
     2461 gtcatatggt tattcttgcc ggaagtggtt tacgccagct gcctgattct tcatacctta
     2521 tggggactgg tcgactgggg gccatttcat aactatgcag caccacgata caacctgctt
     2581 gccatgaccg gtaacaacgc cgccaacata agctatagcc agtgggttaa cgtcatggag
     2641 cagaccatag gtattctgtg gatgtacctc ttacctgtga cactctggtg tttgtgggaa
     2701 tggtatcaac atccgggaca aagccgtttc acccgcagac cagtagatat tacccgactg
     2761 ccacacatct ttgcttcgtt gtcccctgct atcgcccctg tactggcaga tggtgatccg
     2821 gaaaaactct ttcatggtgg caaacgacct gaacggcggg ttgctctcac cccggaagca
     2881 tttgtcgagc agcatactct catcaccaat atgcagctcg atgttgcagc tgcaagacga
     2941 tgtttcatgg cccagttggg taaaccttta acgtcctgga aagacatggc accacatgaa
     3001 aaagcgctct ttgccatatt tggtcttcag tatttcctgg atgatcgtaa ggcagcatta
     3061 aagctcatgg atacactgaa cctctcatgc agaattaaaa gcaaacggga cagtgggaaa
     3121 ttctgtaccc ctgtttattc actggccaaa tcagctttcc agagagtgat taaaagcaac
     3181 ggagcacagc aatggctgaa acaacatcgc tacgtccgca gtgggctggt ctggctgtat
     3241 gcacatgacc tgcggcttac gccacccaac tggatttggc tgaaaggcgt ggacaggacg
     3301 ctgttttatg cattacaccg cgccaatacc acgaaaggat ttattgaagg cgctggcgtg
     3361 gtggccgttg cacgtgcaga ggccgaagcc atgcgttttg gtcttccctg ccctgaaccc
     3421 tgtgttgatg aagcggtgga aggcttacgg cgggatatgc tcagtctcgg tcttatctgg
     3481 gatgaaccac aaccagatcg cgaccggaaa cgccgcatcc tgaccaactg gtccctcacc
     3541 gacgacatcc tgccccgcac acctgctaca gacaacgaat tctgattatc cggagaactg
     3601 aaaatgacaa tcgagtactt tgcaacacgc attgcaaaac atatttccgt gcaggcaatc
     3661 tggccgaatg gcaggacaga aattattgcc gtccttccac gggatgccct gtcagggctg
     3721 gtaacacgaa ataatcaact gattcatata gcctttgacg ccatgggcac ccgtaacaga
     3781 gaaaccgtcc tgattgataa acaggagcat aacgctgacc agatacttac agctattgat
     3841 tcctgcctgc gccttgaata tctgatggaa cgccgcttca gcagaacacc tttgttccgt
     3901 ggcataatcg ccagcgttgt gctgttcgta atggcaacga ttggcttctc tctgttccgc
     3961 tatgttgatc gtgtattctg ggatgataca acgcctgaag cagtacagac agcaggtgag
     4021 ccacgactgc ttccccctca cctgaatcac actgtgccat taaatgaagg cattcaactc
     4081 ccggttcccc cgaaaaaaga cgtccaggta ccggagaaaa caatcacaag caaaaatccg
     4141 gaagccgcag ctgcgcgaca taatctggct actgttctga agcggaacgc cgatcggggc
     4201 atgtttacca tcaacctgtc atccggtcat gaacgaaccc tgttcgcatt tctcgatcct
     4261 gcctgtccta actgcagact tctggagccg gccttaaaac gcctggccag cgacttcaat
     4321 gtcgttatct acccggtttc agttattggt ggagaggaat ccaccgaccg tgtggcacca
     4381 ctgctgtgtg aaaaagatgc tcagaaacgt gctgccggct ggcaccgact gtattccgca
     4441 gataacggaa tgatgacgcc atcagaagaa acaacgcctg cagatgaaac atgcctgaaa
     4501 gcagccagag ctgcgattga tgttaacaat gtcgccttcc gtaagttcgg atttgccggt
     4561 accccatggg tgctgtccga cacgggctgg catctgccta ctggaattct gcaggaaact
     4621 ggcacgctta atttattcct gaaaaccacc gacagcgagt ctggccatga gtgaacatcg
     4681 cgttaacccc gagcttttac accgtacagc ctggggaaat cctgtctgga atgcactcca
     4741 gagtctcaat atctatggct tttgcctggt ggcaagtctt gtggccagtt ttatctggcc
     4801 acttgcatta ccggcatgtc tgttgttcac actgataacg atgctggtat ttagtctgca
     4861 gcgatggcgc tgccctcttc gtatgcccat gacgctggaa tgtgctgacc cgtcacagga
     4921 cagaatgatt aaaagaagtc ttttcagctt ctggccaacc ctgttccagt atgaggtcat
     4981 tctggaatca ccggccagcg gcatattcta tgttggttat caacgcgtca gggatattgg
     5041 cagggagtta tggctgagta tggacgatct gacccgccat ataatgtttt tcgccaccac
     5101 cggtggcggt aaaacggaaa ccatctttgc ctgggcgatt aacccacttt gctgggcaag
     5161 aggattcacc cttgtcgatg gcaaggcaca gaatgatacg gcaagaacga tctggtatct
     5221 ggccagacgc tttggacgtg aagacgatgt ggaagtcatt aacttcatga acggaggaaa
     5281 atcccgcagt gaaatcattc tgagtggtga aaaaacgcgt cctcagtcaa atacctggaa
     5341 tcccttctgt tacagcactg aggcattcac agcggagaca atgcagtcaa tgctgcccca
     5401 gaacgtccag gggggcgagt ggcagtcccg ggccattgcc atgaataagg cactggtatt
     5461 tggtactaaa ttctggtgtg tgcgcgaagg caaaacaatg tcgctgcaga tgctccgtga
     5521 acatatgact ctcgaaggga tggccaaact ttactgtcgt ggcctcgatg atcagtggcc
     5581 ggaagaagct atcgcaccgt tacgtaacta ccttcaggac gttcccggtt tcgacctgtc
     5641 gctggtcagg acaccatcag cctggacgga agaacccaga aaacaacatg cttatctctc
     5701 cgggcagttt tctgaaactt tcagcacatt cacggaagca ttcggggata tttttgctga
     5761 agattccggt gatattgata tccgtgacag cattcacagc gacaggattc tgatggtaat
     5821 gatccccgct ctggatacat cagcgcatac aacatctgct ctgggacgaa tgtttatcac
     5881 ccagaaaagt atgattctcg cccgtgattt gggctaccgc ctcgaaggaa ctgattccga
     5941 tgcactggaa gtcaaaaaat ataaggggcg tttcccttat ctgtgtttcc ttgatgaagt
     6001 gggggcatac tatacggacc gtattgctgt ggaagccact caggtgcgtt cactggattt
     6061 tgctctgatt ttgatggcac aggaccagga acgtatagaa ggacagacaa cagccacgaa
     6121 tacggcaaca ctgatgcaga acacggggac aaaatttgcc ggcaggattg ttagcgaggg
     6181 tagtacggcc agaacgctga aaagcgccgc cggtgaagaa gccagagcga gaatgaacaa
     6241 cctgcagcgt caggacggca tatttggtga atcatggatt gacagcccac aaatcagcat
     6301 cctgatggaa agcaagataa atgttcagga gttgattgaa cttcatcctg gcgagttctt
     6361 ttctattttc cggggagaaa ccgtaccatc agcctcattc ttcatccctg acgatgagaa
     6421 aagctgcagc agcgatcccg ttgttataaa caggtatatc agcgtggatg cgccacgcct
     6481 ggaccgtctc cgccgtcttg ttccccgtac agctcaacgt cgaatcccgt caccggaaaa
     6541 tgtcagtgcc atcattggcg tcctgactgc gaaaccctcc agaaaaagac gaaaaattcg
     6601 cacggagccg cacacgatcg tggatacatt ccagcaacgc attgcaggac gtcaggcggc
     6661 tatggcaatg ctggaagagt acgacacgga tatcaacgca cgtgaaagtg ccttgtggga
     6721 aacagcggtg aatacgctta aaacgaccac acgggaagag cgacgaattc gctatatcac
     6781 gctgaacagg ccagaactac ctgaaacaaa agaggaaaat cagatatcag tgagagcaga
     6841 aagagcaggt ataaacctgt taacgcttcc acaggacaat aaccacccga cagggcgtcc
     6901 tgtaaacggg tttcatcata aaaaaaccaa ccgtccggac tgggac
//
LOCUS       DAZPAC010000214         6924 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZPAC010000214 DAZPAC010000000
VERSION     DAZPAC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.215"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(38..414)
                     /gene="rnpB"
                     /locus_tag="ACHUPM_003640"
     ncRNA           complement(38..414)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHUPM_003640"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(447..1592)
                     /gene="garK"
                     /locus_tag="ACHUPM_003641"
     CDS             complement(447..1592)
                     /gene="garK"
                     /locus_tag="ACHUPM_003641"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR0871316.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1689..2579)
                     /gene="garR"
                     /locus_tag="ACHUPM_003642"
     CDS             complement(1689..2579)
                     /gene="garR"
                     /locus_tag="ACHUPM_003642"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR0871317.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2609..3379)
                     /gene="garL"
                     /locus_tag="ACHUPM_003643"
     CDS             complement(2609..3379)
                     /gene="garL"
                     /locus_tag="ACHUPM_003643"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFR0871318.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3395..4729)
                     /gene="garP"
                     /locus_tag="ACHUPM_003644"
     CDS             complement(3395..4729)
                     /gene="garP"
                     /locus_tag="ACHUPM_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR0871319.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5104..6675
                     /gene="garD"
                     /locus_tag="ACHUPM_003645"
     CDS             5104..6675
                     /gene="garD"
                     /locus_tag="ACHUPM_003645"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR0871320.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6824..>6924
                     /gene="prlF"
                     /locus_tag="ACHUPM_003646"
     CDS             6824..>6924
                     /gene="prlF"
                     /locus_tag="ACHUPM_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFR0871321.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREAL"
BASE COUNT         1703 a         1897 c         1705 g         1619 t
ORIGIN      
        1 aagcaaaaac ccgccgaagc gggtttttac gtaaatcagg tgaaactgac cgataagccg
       61 ggttctgtcg tggacagtca ttcatctagg ccagcaatcg ctcactggct caagcagcct
      121 acccgggttc agtacgggcc gtaccttatg aacccctatt tggccttgct ccgggtggag
      181 tttaccgtgc cacggactgt taccagccgc gcggtgcgct cttaccgcac cctttcaccc
      241 ttacctgatc ccgcttgcgc gggccatcgg cggtttgctc tctgttgcac tggtcgtggg
      301 tttccccccc aggcgttacc tggcaccctg ccctatggag cccggacttt cctcccctcc
      361 gcccgtctcc cccgaagagg acgacgacga agcggcgact gtctggtcag cttcggcgcg
      421 cagtatagag ggtttgcgcg cccttgtcac cccgcgttgc gcattccaat cgccagtgtc
      481 gcggcgatat tacgtgaagc acggcagata ttgtcataag ccccgcggaa tgcttcgtcc
      541 aacgtaccta tgctggtcaa tacgctgaag accgcatcga tgccatgctg atgtacaacg
      601 ccaacatcat cggtcaggct acccgcaatg ccaatcaccg gtttatggta cttcttcgcc
      661 acgtttgcaa cgccaatcgg taccttgccg tgaatactct ggctgtcaat acgcccttca
      721 ccggtgatca ccagcgtaca atcgtgaata tgttcttcca gattcagcgc cgtagtgacg
      781 atttcaatac cacttttcag ttccgcacca agaaacgcca tcagcgccgc gcccataccg
      841 cccgcagctc ctgcgccggg gacatctttc acatcaacat gcagcgcttt tttaatgaca
      901 tcggcatagt gagagaggtt attgtccagc tcaacaatca tcgcttcact ggctcccttt
      961 tgtgggccaa agatgcgcga tgcgccgtta tcgcccacca gcggattggt gacatcacaa
     1021 gcgacgcgaa tgacgcaatc ttttaagcgc ggatcgaggc cggaaatatc aatatcattc
     1081 agagtattaa gactaccgcc gccaaaacca atttcattgc cgttggcgtc gcataatttc
     1141 gcccccagcg cctgtaccat gcctgcgccg ccatcatttg tagcgctgcc gccaatgccg
     1201 ataataatgt ttgttgcact gctctccagc gcctgcagga ttaactcgcc tgtgccgcgt
     1261 gaagtggtta cgagtggatc gcgtttttcc gcaggtacca gctccagccc actggccgcc
     1321 gccatttcaa taaacgcggt tttgccatcg ccggagatcc cccaactggc attcactttc
     1381 tcgcccagcg gccctgtaac ccaggcgtga cgttcagccc cctgggtggc tgcaatcatc
     1441 gcttccaccg ttccttcacc accgtcggca accggaacag aaacatactg tgcatcagga
     1501 aaaatttccc gaaatccttt ttctatcgcc tgcgcaacct cgctggcaga taaactttct
     1561 ttataagagt ctggggcgat tacgattttc atacctatgc ctgttaccac atgacgccgg
     1621 agggcgtttc tcttattcgg cctagattcc aggcccggat tgcaatacac catccgggca
     1681 cgacgtcatt aacgagtaac ttcgactttc gccagttttt cgtagtagca cgccagggcg
     1741 ctatgatccg ccgttcctaa accatctgca cgcagtgcct gcatcatctc cataaccgca
     1801 gctgtgagca gcagttgtgc gccgacgccg tgagaagtat ccagcgcatt cgccagatcc
     1861 ttaatatgca gatcaatgcg gaagcccggc ttgaagttgc ggtccatcac catcggcgct
     1921 ttggcatcca gcacggtact gcccgccagc ccaccgcgaa ttgcctgata aaccaggtcc
     1981 gggttaacgc ccgctttggt tgccagcgtt aacgcttctg acatcgcagc aatattcagc
     2041 gccacaatga cctgatttgc cagtttggtg acgttacctg caccgatttc cccggtatgc
     2101 accacggaac ccgccatcgc tttcatcaaa tcatagtatt tgtcgaaaat agccttgtcg
     2161 ccgcccacca tcactgacag cgtaccgtcg atggctttcg gttcaccgcc gctcaccgga
     2221 gcatccagca tatcaatgcc tttcgctttc agcgcttcgc tgatttcacg gcttgccagc
     2281 ggtgcgatag aactcatatc gatcaatacc gtacctggct tcgcgccttc aataatgcca
     2341 ttctcaccca gcgccacctc tttcacatga ggggagtttg gcagcatggt tatgatgacg
     2401 tcgcactgtt cagcgatcgc tttagccgta gacgctgttt ctgcacctgc agcaatcacg
     2461 tcagcaatag cttctgggtt acggtcagca accaccagcg agtaacctgc tttcagaagg
     2521 tttttactca ttggtttacc cataatcccc aggccaataa aaccaacttt catagtcata
     2581 tcaaccatct ctcttgttgc ggtggtggtt attttttaaa ggtatcagcc agtttctgag
     2641 tggcagagcg gaagacgcca agatcgctgc cgacagccac aaacgtcgcg ccccattcca
     2701 gataacgacg cgcatcggct tcgaccggcg cgagaatacc gctgggtttg ccgtgcgcgc
     2761 tggcacggtt aaaaatatgc tgaattgctt tttgtacatc cggatgcgag gcattgccga
     2821 gatggcccaa tgccgcagcc agatcgctgg ggccgacgaa gataccgtct acaccttcgg
     2881 tagcggcaat agcatcgacg ttatctacgc cctgctgact ttctatctgg accagaatag
     2941 taatgttctt gttcgactga gcgaaataat ccgccacggt gccaaacata ttggtgcggt
     3001 gagaaacgga gacgccgcga atgccttccg gcgggtaacg agttgatgcc accgcctgct
     3061 ctgcttcctc ttttgtttct acaaaaggaa tcaggaagtt atagaaaccg atatccagaa
     3121 gacgcttaat aattaccggc tcgttggtcg gcactcgcac tactggcgcg ctggcgctgc
     3181 ctttcagggc cattaactgc ggaataaacg tggaaatatc gtttggcgca tgttcgccat
     3241 ccagcaccag ccagtcaaac ccagccaaac caagaacttc agtgctaatc gggttagaga
     3301 gtgctgacca gcaaccaatt tgtacctgtt tcgcagccag tgcggctttg aatttattcg
     3361 ggaaaacatc gttattcatc gcttatacct ttgcttattt ctgcaattcc atacgtttaa
     3421 tgtcgccaac tacgaagagg tagcagacca tcgccatcag cgctgaacat cccacgaaaa
     3481 ccagtgctgc attgaaggag tgcagttcac ttaccaggta gccaatcacc agtggagtga
     3541 caatggaggc aacattgcca aagacgttaa agacgccgcc acagaggcca acaatctctt
     3601 tcggcgcggt gtcagaaatc accggccagc ccagagcacc aaatcctttg ccaaaaaaag
     3661 ccagcgccat cagcatgacc accagcgtgg tgttgttggt gtagttacat aagatgatgg
     3721 tggaaaccag caacattccc agcacaatcg gtagcttacg tgccagggtc agggataaac
     3781 cgcgtttgat cagataatcc gagaagacac ctcccagcac gccgcccgca aaaccacaca
     3841 gtgccggaat cgaggcgacc agacccactt tcagaatcga catgcctttt tcctgcacca
     3901 gataaatcgg gaaccaggtg aggaagaacc aggtgatggt gttgataaaa tattgtccga
     3961 aaaatacgcc caacatcatg cggttagaga gcaattgctt gatgtaatgc agtttgggac
     4021 cgcttgctgc cgcgctgccc ggctttttgt ggtccatatc gaccaccgcg ccattttcag
     4081 agataaactt cagctcttcc gcagacatac gtgggtgatc tgtcgggtta tgaatcaact
     4141 tgatccacag cgccgtcagc acaaaaccaa tcccccccat aacggtgaag acgtgctccc
     4201 agccccaagc gaaagtcagc cagccaagca gcggcgaaaa gagcgccagc gagaaatatt
     4261 gcgccgagtt aaagatggcg gaggcagtac cacgttcttt cgtcgggaac caggcggcga
     4321 caatacgggc gttcgccggg aatgatggcg cttccgagaa ccccagcata aagcgcataa
     4381 agaacatgga gatccctgcc caggccagcg ggaacatatc aacaaagcct tgcaggaagg
     4441 tgaacagcga ccagaaaaag aggctgtagg tgtaaacttt tttcgagcca aacttatcaa
     4501 gcaaccagcc gccggggatt tgcatcagca agtaggccca gccaaaagca gagaagatgt
     4561 aacccatcga aaccgcactt aactgcaact cttttgccac ttcggtacca gcaatagaca
     4621 gcgttgcacg atcggcgtag ttaacggcgg taacaataaa aataatcagt aatattaaat
     4681 agctggtatg cacgcctttc ttttttacgt caacggtgtc cagaatcatt ttatttacct
     4741 cgggtactta tgctgatttt tattattatg gggaaggtgt tatttatgag ttttatttat
     4801 gccgttacga caatgaactc gggaattagt ataagcagcg ggagaataat aatcattgtg
     4861 caaatgctaa tttaatcagt actatttaaa tattattttg agcatatgca cataaggttg
     4921 cgcgctaaag cacagatttg cgctttacct taccgggcgg cactgcaatc cctgaaatga
     4981 ttgacattga tcacatttct gcgtttaaac tcctgacatt cttatttcac ccaatgaagt
     5041 catttatttt taaatgagac cagatcctca ttttaataac ccctggctgg agaatattgc
     5101 acaatggcca acatcgaaat cagacaagaa acgccaactg cgttttatat aaaagttcac
     5161 gacacagata atgtggcaat tattgttaat gataatggcc tgaaagcagg aacgcgtttt
     5221 ccggatgggc tggaattaat tgaacatact ccccaggggc ataaagtcgc attgctggac
     5281 attccagcta atggtgaaat tattcgttat ggcgaagtga ttggttacgc cgtgcgcgca
     5341 atcccacgcg gaagctggat cgacgaatca atggttgtac taccggaagc gccgccgtta
     5401 cacacgctgc cactggcaac caaagtcccg gcccccttac cgccgctgga aggatacacc
     5461 tttgagggct atcgcaatgc cgatggcagc gtgggcacca aaaacctgct cggcatcacc
     5521 accagcgtcc actgtgtggc aggcgtggtg gactatgtag taaaaatcat tgaacgcgat
     5581 ctgctaccga aatacccgaa cgtcgatggc gtggtggggc tgaatcattt gtacggctgt
     5641 ggcgtggcga ttaacgcacc ggcggcagtt gtgcctattc gtaccatcca caatatttcg
     5701 ctgaacccta actttggcgg cgaagtaatg gtgattggtc tgggttgtga aaagttgcag
     5761 cctgagcgcc tgctggtcgg cacggatgat gtgcaagcta ttccagtaga aagcgccagc
     5821 attgtcagtt tgcaggatga aaagcatgtc ggctttcagt ccatggtcga ggatattttg
     5881 caggtcgccg aacgccatct acaaaaactg aatcaacggc agcgagaaac ctgcccggct
     5941 tcagaactgg tcgttggtgt gcagtgcggt ggcagcgatg cgttttctgg tgtaacggca
     6001 aacccggcgg ttggctatgc gtctgatcta ctggtgcgct gcggcgcaac ggtgatgttt
     6061 tcagaagtaa cggaagtgcg tgacgcgatc catctgctga caccacgcgc agtgaacgaa
     6121 gaggtcggca aacggctgct ggaggagatg gagtggtacg ataactatct caatatggga
     6181 aaaaccgacc gcagcgccaa cccttcgccg ggcaacaaga aaggcggtct ggcaaatgtg
     6241 gtggagaagg cactcggctc cattgctaaa tcgggtaaaa gtgcaattgt tgaagtgctg
     6301 tcgcccggtc aacgcccgac taaacgcgga ttaatttacg ccgcgacgcc agccagcgat
     6361 tttgtctgtg gcacgcaaca ggtggcttcg ggtatcaccg tacaagtgtt tacgaccggc
     6421 cgtggtacgc cgtacggcct gatggcggta cccgtaatta aaatggcaac ccgcaccgag
     6481 ctggcgaacc gctggtttga tttaatggat attaacgcgg gcactatcgc taccggcgaa
     6541 gagagcattg aagaggtggg ctggaagttg ttccacttta ttctcgacgt cgccagcggg
     6601 aagaagaaaa ccttctcgga tcaatggggg ctgcataacc agctggcggt gtttaacccg
     6661 gcaccggtga cctgatttct tttggaacgt ccctcgcaaa acatggcctt agtgccatgt
     6721 ttttattgtt taaagccccc acgtccatta ataatgcatt tgcattacct tagttcaagc
     6781 ttataatttg agcagaaaac aggctgtaaa aggacaatga atcatgcccg ctaatgctcg
     6841 ctctcacgct gtactgacca ctgaatcaaa ggtcacgata cgcggacaaa caactatccc
     6901 cgcgccagtg cgtgaggcct taaa
//
LOCUS       DAZPAC010000215         6868 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZPAC010000215 DAZPAC010000000
VERSION     DAZPAC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6868
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.216"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(270..1013)
                     /locus_tag="ACHUPM_003647"
     CDS             complement(270..1013)
                     /locus_tag="ACHUPM_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0871322.1"
                     /translation="MPSIPSFVLSGLLLLCLPFSSFACATTSHISFSYAARQRMQNRA
                     RLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALA
                     ENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAK
                     LPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGE
                     TIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            1229..1489
                     /gene="dinJ"
                     /locus_tag="ACHUPM_003648"
     CDS             1229..1489
                     /gene="dinJ"
                     /locus_tag="ACHUPM_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFR0871323.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            1492..1770
                     /gene="yafQ"
                     /locus_tag="ACHUPM_003649"
     CDS             1492..1770
                     /gene="yafQ"
                     /locus_tag="ACHUPM_003649"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFR0871324.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            1926..2666
                     /gene="dpaA"
                     /locus_tag="ACHUPM_003650"
     CDS             1926..2666
                     /gene="dpaA"
                     /locus_tag="ACHUPM_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR0871325.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(2637..3404)
                     /locus_tag="ACHUPM_003651"
     CDS             complement(2637..3404)
                     /locus_tag="ACHUPM_003651"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR0871326.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="ACHUPM_003652"
     CDS             complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="ACHUPM_003652"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR0871327.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4428..>6868
                     /gene="fadE"
                     /locus_tag="ACHUPM_003653"
     CDS             4428..>6868
                     /gene="fadE"
                     /locus_tag="ACHUPM_003653"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR0871328.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
BASE COUNT         1680 a         1734 c         1808 g         1646 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataaac cattattata aaattaataa atatatttca gtttattatg taaaaacatc
     1141 cgtttattaa attactcatg gattgtcgct gttgctcatt tgagctacaa ttcaagctga
     1201 ataaatatac agcacaggag atcccccaat ggctgctaac gcgtttgttc gcgcccgaat
     1261 cgatgaagat ctgaagaatc aggcggcgga cgtactggcc gggatggggc tgaccatctc
     1321 tgacctggtt cgcataaccc tcacaaaggt cgcgcgtgaa aaggcattgc cgtttgattt
     1381 acgcgagcct aatcaattaa ccattcaatc aatcaaaaac agcgaagctg gcgttgatgt
     1441 tcataaggcc aaagacgctg atgatttatt tgataaatta ggaatttaaa tatgattcaa
     1501 agggatattg aatactcggg acaattttca aaggatgtaa aacttgcaca aaagcgtcat
     1561 aaggatatga ataaattgaa atatcttatg acgcttctta tcaataatgc tttaccgctt
     1621 ccagctgttt ataaagacca cccgctgcaa ggttcatgga aaggttatcg cgatgctcat
     1681 gtcgaaccgg actggatcct gatttacaaa cttaccgata aacttttacg atttgagaga
     1741 actggaactc acgcggcgct ctttgggtaa agcacgctgc cgatgaaatt tgtaaaccct
     1801 gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca aatccagact
     1861 attccgattt gggatctatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa
     1921 gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg
     1981 ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt
     2041 tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctctacg
     2101 tcaaaatggg cgagcaatat cagctgctcg acagttataa aatctgtaaa tattccggcg
     2161 gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc
     2221 agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt ggtttcccca
     2281 atgcctatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac ggcgattgtg
     2341 tttccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc cagttcgtta
     2401 ctggtgcgct ggtgtttggt cagccgagcg tgcaagtgag tatttacccg ttccgcatga
     2461 ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcaactga
     2521 agccgggtta cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg
     2581 gtcgttacgt ggtcagcaag ccgttaagcc acgaagttgt gcagccacaa ctggcatcaa
     2641 actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatctt ttgccaggtt
     2701 tcattgcccg tcagcggctg tgttgcaatc accgtgacca catcattcgg tgtggtctgc
     2761 gagctgaagt cgatttccac atcctgatcc agcagcgttg ccacgccaaa cggcgcgcgg
     2821 cgggtgatcc agtgtaaatt agtcgagcaa tacgccatca catagcgccc gtcagaaagc
     2881 agcatgttga aaacgccctt ctgccgcagt tcatccgcca gtgaggcgat atatttaaac
     2941 actgccgcca tgttgcccgg tgtgcgcggg taacgctgcg ttaatttatg caggagccag
     3001 cagaaggctt tttcgctatc ggtttcaccg accgggcgga agttgccggt ttccagtgat
     3061 ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca taactcacgg
     3121 gtaaacgggt gagtattttc cagcgccacc tcgccccgat tggcctggcg aatatgagcc
     3181 accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg ggaattaaag
     3241 ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag
     3301 ccatctttat gtggcccggt tccaccacca cgctgcacca gaccggtgaa actaaagcag
     3361 atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa cctccacaac
     3421 agcgggagcg catggccccc ggcgaagatt gatttaagaa ttttcaggcc ggatgcggcg
     3481 taaacgtctt attcggccta cggttcgcgt acagatttgt aggcatgata agacgcatca
     3541 gcgtcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct tatccggcct
     3601 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
     3661 aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa tttcgatatc
     3721 cgccgtgcca gccattttac cgccgtcttt accggtcagg gtgatcactt tcatcccctt
     3781 ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
     3841 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa tatcattgaa
     3901 accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa tcgccgggta
     3961 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tagcgtcgca
     4021 atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
     4081 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
     4141 cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga ggatatcctt
     4201 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
     4261 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
     4321 agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc actacaacca
     4381 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
     4441 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
     4501 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt ctgtggtcag
     4561 cgtgggtact ggtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
     4621 agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
     4681 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
     4741 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gccgaagagc
     4801 aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat ttccagatta
     4861 ctcatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag catcgcttct
     4921 tcgcgatgat tatcaaaaaa gagtacggcg gactggagtt ctcagcttat gcccagtctc
     4981 gcgtgctgca aaaactctcc ggcgtgagcg ggatcctggc gattaccgtc ggcgtgccga
     5041 actcattagg cccgggcgag ctgctgcaac attacggcac tgacgagcag aaagatcact
     5101 atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgaca agcccggaag
     5161 cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc gaatggcagg
     5221 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctatattacg ctggcaccga
     5281 ttgcgactgt gcttgggctg gcgtttaaac tctccgaccc agaaaagctg ctgggtggcg
     5341 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
     5401 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
     5461 tcttcgtacc gatcgattac atcatcggtg ggccgaaaat ggccgggcaa ggctggcgga
     5521 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
     5581 gcgtgaaatc ggtagcgctg gcaacgggcg cgtatgctca cattcgccgt cagttcaaaa
     5641 tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc ggtaatgcct
     5701 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc gaaaaacctg
     5761 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag tcgattattg
     5821 atgcgatgga tatcactggt ggcaaaggca ttatgctcgg gcaaagcaac ttcctggcgc
     5881 gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt ctgacccgca
     5941 gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg gaagagatgg
     6001 aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa catatcggtc
     6061 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
     6121 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
     6181 acctcgccct gctttctgat gtctcgatgg cagtgctgga cggcagcctg aaacgtcgcg
     6241 agcgcatctc ggcccgtctg ggggatattt taagccagct ctacctcgcc tctgccgtgc
     6301 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
     6361 tacaagatgc gctgtatcag gctgaacagg cgatggatga tttactgcaa aacttcccga
     6421 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
     6481 caccttctga caagctggat cataaagtgg cgaagatttt acaagtgccg aacgccaccc
     6541 gttcccgcat tggtcgcggt cagtacctga cgccgagcga gcataatccg gttggcttgc
     6601 tggaagaggc gctggtggat gtgattgccg ccgacccaat tcatcagcgg atctgtaaag
     6661 agctgggtaa aaacctgccg tttacccgtc tggatgaact ggcgcacaac gcgctggcga
     6721 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
     6781 gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg gtaaagttgc
     6841 cggagaaagt gcggaaagtt gaagccgc
//
LOCUS       DAZPAC010000216         6820 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZPAC010000216 DAZPAC010000000
VERSION     DAZPAC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.217"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..213)
                     /gene="cas6e"
                     /locus_tag="ACHUPM_003654"
     CDS             complement(<1..213)
                     /gene="cas6e"
                     /locus_tag="ACHUPM_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR0871329.1"
                     /translation="MAVWGTHRQSGAVAQQGGTMYLSRITLHTGQLSPAQLLHLVDRG
                     EYVMHQWLWDLFPGGKERQFLYRREEL"
     gene            complement(138..884)
                     /gene="cas5e"
                     /locus_tag="ACHUPM_003655"
     CDS             complement(138..884)
                     /gene="cas5e"
                     /locus_tag="ACHUPM_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFR0871330.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(895..1950)
                     /gene="cas7e"
                     /locus_tag="ACHUPM_003656"
     CDS             complement(895..1950)
                     /gene="cas7e"
                     /locus_tag="ACHUPM_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFR0871331.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1962..2498)
                     /gene="casB"
                     /locus_tag="ACHUPM_003657"
                     /gene_synonym="cse2"
     CDS             complement(1962..2498)
                     /gene="casB"
                     /locus_tag="ACHUPM_003657"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFR0871332.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2495..4056)
                     /gene="casA"
                     /locus_tag="ACHUPM_003658"
                     /gene_synonym="cse1"
                     /pseudo
     CDS             complement(2495..4056)
                     /gene="casA"
                     /locus_tag="ACHUPM_003658"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            complement(4154..>6820)
                     /gene="cas3"
                     /locus_tag="ACHUPM_003659"
     CDS             complement(4154..>6820)
                     /gene="cas3"
                     /locus_tag="ACHUPM_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFR0871333.1"
                     /translation="KNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKR
                     NCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKN
                     YEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGWPPDRMD
                     ELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADW
                     TGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGINTLFPFIENPT
                     PLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMAT
                     ANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFS
                     QGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACD
                     AYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGLQEAPFLEKDDY
                     PWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSV
                     DDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKEDSSQRAGKVLI
                     CTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELL
                     ILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESV
                     YGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAE
                     ESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIE
                     QRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         1650 a         1857 c         1617 g         1696 t
ORIGIN      
        1 gagttcttcc cgacgataaa gaaattgcct ttctttgccg ccagggaaaa gatcccacag
       61 ccactgatgc atcacatatt caccgcgatc caccagatgc agcaactgcg caggcgaaag
      121 ctggccggta tgtaaggtta ttcttgagag gtacatggtt cctccttgct gagccacggc
      181 ccctgattga cggtgcgttc cccaaacagc cactgctgac gatttaaagg aacatcccgg
      241 cggcgtaata ttttgctcgc gaccaggccg tcgtgtttcc cttcccacca gcattcatcc
      301 tgaagtttcg ggagtgagac tttcagttta tggaaatgat cctgatactg ttggtatgca
      361 ttacataagg catcacacgc gttgccttcg agcagtaacg gcgcaagagg taatgccagc
      421 ggatgacttt ttcgccccag ataaagcgga aaaactggat ggcgtaaacc gtcctgcaac
      481 tgttcgaggc tgtaaggcgc atcggctgtt gttgccaccg ccaccatcca ccaggcatcg
      541 gtgtagtagt cgcgccggga gataatcgcg ctcaaaagct cagggtcgct caactcttca
      601 cgacggctga aataacgtgc tttacgcacc tcttttggca tctggaccgt gtgataatcc
      661 cgtgcccagc gcgggttacg gctggcgcaa accaccagtg aatagtggcg attaaacgcg
      721 tttaagcgtt cggtatcatc acgccgaatc cctaccccgg cagccagtag ccccagcaat
      781 gctgagcgcg aaggaagttc atgggtatga cgaacttcac ctggagcatc gacaccccag
      841 gacgccatcg gcccatgaag ctgaaaaatc agatattggc tcataagccg ccccttacgc
      901 gcagataaag tccagcacgt ccttcatgct tccctgtttg ttcatcacgt caaaacttgc
      961 gcattcggtc ttctgttcgt agaccgtatt catgttttcg cgaagcgttg taatacgctg
     1021 caccgccaca tctaactgac gtgtgccatt aatgggttca tagaaagccg ccgccagaga
     1081 gcgtggttga tcggtgcctt tttctgccag cgcccaggaa gcataggcac ggctggcaaa
     1141 gctattttgt ttaccggttg gggaaacttt aagcgcggct tccgtaaaag cgcgtatggt
     1201 ctgattagct aacgcttcgt caccgccgag gttttctacc agcagatctt tatcaatgca
     1261 gatataggtg tagaaaagtg ctgaaccgaa tccggtttct ccgagatgcc cggcaccggc
     1321 atcttcagag gcctggcgca aatcatcaac ggcggtgaaa aaatcatctt cgacaatcgt
     1381 ttcactgaca ccaaacgcat gcgcgacctg gcaggcggct tcaacattaa actcaggttt
     1441 attcgccagc atacgaccaa acatagcgat atctaccgct atacggtctt tacgtaacaa
     1501 cgcgagatct tcctcttttg gcgcgcgctt ttcttctgcc agttgatgag caagcgcttt
     1561 tacggcgtca aattctgccg ggctgatatg aactaattgt tcagtttcgg cgttagtgag
     1621 cggatctttt ggttttttgt catttttagc tttcccaaga taatcagcaa tttttgccgc
     1681 ccattcgatg gcttttttat cttcaatccc tttctcaatc aggatagttg ccgcctcacg
     1741 cgcaatacgc ccactgcgaa taccaatatg gcccgccagt gcctgttcaa aaagtgcaga
     1801 agtgcgccac gcacgtttca gactttgcga ggaaacgcgc agacgcgttg ctccacccag
     1861 caccacagtt tttggcgcac cggtgtcatc acggttaagg ttggcagcag ggtaagcggt
     1921 taacacatga agctgaataa atgttgtcat gagatagtcc tttattgatt ttgttcgtta
     1981 tcggcgtcgc cagcctggta atattccagc gcccagcgga tacggataaa ttcggttggc
     2041 cgctgctggc ggcggtgatg attgagcagg tcgtcgctct cctggcacca gcggaaaatg
     2101 ccatctgcca gggagtcaag attgacggaa ccgtttaata atctgacggc gcggcgtaac
     2161 tggcgcagta attcatccgg tgtttttact gctgacaggc gagtaaaacg cccctttgac
     2221 attaccgccg ccagttgcgc ggcaaaaggc tgccgttcat caatggcttt aacattggcg
     2281 ctaactgcgg ctatcagcgc cagtgcggtg atacgccact caggttcatc ctgccacttt
     2341 atttgtctgt tcttcagaaa caggcgaaat ccatccgtca gacaaacatc attaaccgtc
     2401 gtactacgcc gcagactggc acgttcgccg cgtttctcct gcaattcctc gtgccatttg
     2461 cgcagcgtgg ctttgtgctc ctcttttaca atactcattc agcagcctcc tgctttttct
     2521 ccctggcggc tttagcactt tgcttctccg ccgatgttgt aaaatatttc ttgcgcgcgg
     2581 tcatgatgcg ttccaaatca acgggctcat aaggattggt gaatacacgc tcgtcaaaat
     2641 cctgacgcgc gaataaccaa atttccttat tccatttgct gagtaattca tccgcatcct
     2701 gaccttcttc aatttggcgc actaacctca ggaagcgatg ctgagttttg ttccagaagt
     2761 cgatatccac aaaactaaaa tcaccccttg caccttttgg atcggagaac catgcttctt
     2821 tcaatgcact ccgtaacaga cttagaatcc gtgaagccgt ttgcgcagcc agccgcagtt
     2881 tcggtatctg gccttctttt tattgagcag cagcgggaaa tgatgttcgt accaacagcg
     2941 cgctttcatg ttgtcgaaat catagccaaa tccccacagg cccacttttg cctgcttgag
     3001 actgctggca ttaaagagtt tcaccaccag cgcgggaagt tccgtattgt tttctgactt
     3061 acccgtttcg ataaggccta accagtcgcg ccagattaaa ccgcccggtt gtggtttgac
     3121 cgagtaaaac tcgccgccct ctttaagtag tacacggtaa ggcgttaagg gatgctgcca
     3181 catggcatag ttagcgccgt aatttttggt cgtcatcagg ctcagaagcg cgtcgctctg
     3241 ctcaccgcaa atatcgcagt tgccgactgt ggtggtatta aaatcaatac gaatacggcg
     3301 aggcattccc cagtacgcct ggagtttatt gacctgctca tcggttacca ccgcaccggc
     3361 cagttcgctg atgcgcgtcg ggccaagcca ggggaaaacc agatcgtcaa attttttggg
     3421 tagcggtaaa tcggcttcat cctgcggcat gacattgatc cacagtttgc gccacaaggg
     3481 agtttgttga ttaccctgat attcctgtaa ttcaatcaga gtggtcatcg gcccaccgcc
     3541 gcgtaaaccg gtgcggtagc ctttgccacc tgacggcgca ttcaactgta gggagaacag
     3601 agctaacgca gagcaatgtg ggcacaggta ttccgttaca ccacgcttga taaagtggtc
     3661 tttattaaac ttcgttgttt gagcgccggg aatctcaggc agtagcgaag cgaccggaac
     3721 tttatctccg gtgagcgcct cgaaatcctg tataaatgaa ggggaatctg gcccaaactg
     3781 gaaagcatgt tctaatgaca gcaatgcttc ccgtagcttt tcagcttcca gtccgtcttc
     3841 ccagatatca tcccaacgac gatgatcttt tggcgcgaag ctggtttgta gtagccccag
     3901 caaaaactgc cataccgcac cctggagatc tgcccgcggc gcagaaatat cgacaacatt
     3961 ttcatccgcc agatcgactg gcgccagttt gcctgtcgtt ccgtctttaa aacgaacggg
     4021 caaccacggg gttgtcagaa gtgaaaacga gttcatacgc atcctctcgt aaaaaagacc
     4081 atcccaggtc ggttgtccat ctttccatcc ttttcggtgt atcaactatt cagagagtcc
     4141 tgtcgcagac atctcaacca accttgccaa tcaatccttc cgtagccgaa tacccacaac
     4201 tttcgtcatc gttgactaaa ataacgtttg ccatctccgg atcttgtcgc tgttcaatgc
     4261 accacagcct gaacgcctct ccctccagta aagaaaactc atcccgatgt tttttccacc
     4321 agcttcggcg cactctgaca acgctcattt cccatgcgtg agcaccggtg gcataaggct
     4381 tcaccacacc gtcaatacag gtagccagcc acagggaaac agactcctca gccagacgtg
     4441 tcgacagttt ttctgggagg taatcgttga tattggcggc atagccgggc ctgaagttca
     4501 gcacaaactt tttagccatt gcgcgatcac agtaatattt gcccacttgc tcctgctcgc
     4561 tgcgggcaaa tccttccggc attaccacgt cctcaccgta gacggattca ataagaagcc
     4621 gggctgcgtg tggcatctga atagcacctt gctcacgcag cacacgctgc gtcagccaga
     4681 ttcgcccatg atcgggatag acaaatgcac tgttacgcat ggcactgcca aaccattcgt
     4741 cgccaggaga gtcgtcccag acgggagcca gaatcagcaa ttcagggggg gaacgctcgt
     4801 cctttccgtc acgctttaac tgaccattaa tatcgcggat atgccgctgt aatcgtcctg
     4861 ctcgctgaat cagcaaatca acaggggcca ggtcggagat catttcgtcc aggtcacaat
     4921 cgacactctg ctctaatacc tgagtacaaa taaggacttt tccggcacgc tgtgagctat
     4981 cttctttacc aaagcgtgcc agtgtctccg tttcaattcg ctggcgatcg ctaaaagcaa
     5041 agcggctatg aaaaagtgaa aggctggaag caggaatgac gccgcgggca agcagctgac
     5101 gatgaacctt aatagcgtca tcgacagaat tccggatcca ggcgatgcat ttcccctgac
     5161 ttaccgccga ttcgatacgc gcaatacact cttgttcact atgaagccaa cccacgctga
     5221 cgctacgctc aacgtctttg cgcgtcgcta cccggtgtga gttcacatcg gatttcgtga
     5281 catgcgtcag ccaggggtaa tcatcctttt ccaggaacgg agcttcttgc aggccctctg
     5341 taccccgcgc aaaggcagcg acgagtttgt cgcgctgctg ttgagataac gtggcagaaa
     5401 gcaaaataac gctgtttccg ccacgcgcct gccgctcgat caacccttca agaatgcacg
     5461 acatgtaagc gtcacaggcg tggatctcat cagccagcag gattttgtta ctcagcccca
     5521 gaagccgcag attattatgt ttgaacggca tcaccgccat catcgcctga tccagcgtgc
     5581 cgacgccaat ttcagccagc aacgccttct tgttactgtc ggcaaaccag gccgcacatc
     5641 cctgactgaa cgtttgttca tccggttctt ctgacccgac taaatcacca gaccagagtg
     5701 attcattaaa gcggtccatt aatgtgcggg cactatgtgc cagcaccagg ctggggcggg
     5761 attctggcga atagaaagca agccaggttt tgaccagccg atcgtacatg gcattggccg
     5821 ttgccattgt tggcagacca aaaaacagcc cctgagcctt ccccgcagct atcaacctgt
     5881 gcgccaggat aagcgccgct tctgttttac ctgcgccagt cacatcttcc agaataaata
     5941 actgcggtcc ttgctggctg atatccagat ccagcacctt ttgctgtaac ggtgtcggat
     6001 tctcaataaa aggaaacagc gtattaattc cgttgaaagg cgcgactttt gcttttagag
     6061 gaaagacggt taaggcgttt tgagcctgaa ctaaagtttt ctgccagtaa cctttaatat
     6121 ccattgggtg tgcgacgcgt ggaaaaaatc gcgttgacga acccgtccag tctgcgagca
     6181 cgacggttgc agagatatac caggaaaggt gttttataag ttcaatgccc tcatcatcat
     6241 cccagaatgc ggggatctct atgagcggaa acagtggttt gatttcaagg agaaaatctc
     6301 gtgctgcagc tttgtcttca ggcagaaaat tatccaattc atccatacga tcagggggcc
     6361 aaccatgatg ccctgtcgtc acgggcatcc acatttctat cactcgccta agtttgcgcg
     6421 gagagagcga agatgaagga agtaactcct gacattcact taaataatga ttccacaacc
     6481 agtagcccag cgttgaatga gagatttttt cgtaattttt tctggcacct tcctgaatct
     6541 tgagttcagg gggcaggtaa agttgttgaa aagagcgggc aaattttcca atatcgtgcc
     6601 agcacagcaa ccaggcgaaa aattgagccg cctgttcctt gtcagaaatc cctaattgac
     6661 gaaaataatc agccagcccg aagcaatttc ttttaaccat taaatagccc atcgcggcta
     6721 catccagcga atgccagcaa agaaggtgat agtcgtcgcc cccgtctttc tcgccgcgtc
     6781 gggtttttcc ccagaaatca aaaaaagtca caatattttt
//
LOCUS       DAZPAC010000217         6808 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZPAC010000217 DAZPAC010000000
VERSION     DAZPAC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.218"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            52..936
                     /locus_tag="ACHUPM_003660"
     CDS             52..936
                     /locus_tag="ACHUPM_003660"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR0871334.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="ACHUPM_003661"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="ACHUPM_003661"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFR0871335.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="ACHUPM_003662"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="ACHUPM_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR0871336.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="ACHUPM_003663"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="ACHUPM_003663"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR0871337.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="ACHUPM_003664"
     CDS             5160..6443
                     /locus_tag="ACHUPM_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFR0871338.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..>6808
                     /locus_tag="ACHUPM_003665"
     CDS             6590..>6808
                     /locus_tag="ACHUPM_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR0871339.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCIT"
BASE COUNT         1790 a         1671 c         1631 g         1716 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaagtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacataatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta cccacgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 ctgtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgccc gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccgat acccggggtc actgtcaggc
     2221 cgccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agtcagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccaat aagcggtaat tgaaagtttg tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgt tcaacgacac
     2581 cgacgcctgc gcccagggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg acgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gccttgtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg agcctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcatttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gctatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacaga acccggtgat gagaacaacc aggacatctc
     3781 cgcgctggta gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aatttgtcga
     3901 gatgtttaaa gcaccgatta aagtgctgca tccgctgtta accgctactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgaatcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt acgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aaaggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tagaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct ataacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgaccgggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc attaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccgtatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caggcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccag gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tggtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacgtta attttatttt gcattact
//
LOCUS       DAZPAC010000218         6537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZPAC010000218 DAZPAC010000000
VERSION     DAZPAC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.219"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="ACHUPM_003666"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="ACHUPM_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR0871340.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHUPM_003667"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHUPM_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR0871341.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="ACHUPM_003668"
     CDS             2408..3157
                     /locus_tag="ACHUPM_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR0871342.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3729
                     /gene="yieF"
                     /locus_tag="ACHUPM_003669"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3179..3729
                     /gene="yieF"
                     /locus_tag="ACHUPM_003669"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="ACHUPM_003670"
     CDS             complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="ACHUPM_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR0871343.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5286..5951
                     /gene="yieH"
                     /locus_tag="ACHUPM_003671"
     CDS             5286..5951
                     /gene="yieH"
                     /locus_tag="ACHUPM_003671"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR0871344.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6018..6491
                     /locus_tag="ACHUPM_003672"
     CDS             6018..6491
                     /locus_tag="ACHUPM_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFR0871345.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1607 a         1650 c         1652 g         1628 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactctgtac cgggtgggct gaaaaatgcc atcgactggc tttcccgctg
     3481 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     3541 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     3601 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     3661 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     3721 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     3781 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     3841 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     3901 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     3961 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     4021 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     4081 gacagaaaga taaccagcag gaacaacaga ccaacaacca ctgccgtcag accggtacga
     4141 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     4201 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     4261 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     4321 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     4381 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     4441 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     4501 accgctgcgt gaatgttgcg cgaggccaga atggcgatga tgaagaaacc gaggatcccc
     4561 agcagcacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     4621 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     4681 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     4741 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     4801 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     4861 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     4921 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     4981 aatacgatgt agaccatcgt caggaaggtg gtgaacccgg caatcacttc agttcgtgcc
     5041 gtagtaccat gttcgcgcag tttaaaaact cgttccagca tcccctgacc agaagtctgg
     5101 gtggtatgtt gatgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     5161 tataccaaaa tgcgacaata ggcgcgtttg tgagagactt tttttattgg atttacttat
     5221 acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga ttaaaaagga
     5281 aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg
     5341 aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc
     5401 ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc gatattgttt
     5461 cccttgaaca tggtgttacg ttagcgaaaa cagaatctga acacgtttac cgtgcagaag
     5521 tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctgaagcg ctcctgtcag
     5581 cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg cagcattcta
     5641 tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc tacgatattc
     5701 agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg aatgtaaatg
     5761 tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt atcgacgcag
     5821 gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt cacccgaaag
     5881 tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt ggttgggata
     5941 ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg ctcaaaggta
     6001 agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga ctttattttg
     6061 cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc ttcgcaaaag
     6121 aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta ttgtcgatta
     6181 ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg ccggaaaaga
     6241 tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc gttatcaccg
     6301 agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag ttgtttgaaa
     6361 acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg gcaggtgtgg
     6421 tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct gaaacagact
     6481 ctttaagtta agcgggatac tttatctttg ggctactcaa aagcagacag gatgtta
//
LOCUS       DAZPAC010000219         6532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZPAC010000219 DAZPAC010000000
VERSION     DAZPAC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.220"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..906)
                     /locus_tag="ACHUPM_003673"
     CDS             complement(<1..906)
                     /locus_tag="ACHUPM_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFR0871346.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
     gene            complement(1331..1474)
                     /gene="ypaB"
                     /locus_tag="ACHUPM_003674"
     CDS             complement(1331..1474)
                     /gene="ypaB"
                     /locus_tag="ACHUPM_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR0871347.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            1602..3887
                     /gene="nrdA"
                     /locus_tag="ACHUPM_003675"
     CDS             1602..3887
                     /gene="nrdA"
                     /locus_tag="ACHUPM_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0871348.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            4121..5251
                     /gene="nrdB"
                     /locus_tag="ACHUPM_003676"
     CDS             4121..5251
                     /gene="nrdB"
                     /locus_tag="ACHUPM_003676"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0871349.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            5251..5505
                     /gene="yfaE"
                     /locus_tag="ACHUPM_003677"
     CDS             5251..5505
                     /gene="yfaE"
                     /locus_tag="ACHUPM_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR0871350.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(5559..6208)
                     /gene="inaA"
                     /locus_tag="ACHUPM_003678"
                     /pseudo
     CDS             complement(5559..6208)
                     /gene="inaA"
                     /locus_tag="ACHUPM_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(6288..6392)
                     /locus_tag="ACHUPM_003679"
                     /pseudo
     CDS             complement(6288..6392)
                     /locus_tag="ACHUPM_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6387..6532
                     /locus_tag="ACHUPM_003680"
                     /pseudo
     CDS             6387..6532
                     /locus_tag="ACHUPM_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1686 a         1686 c         1526 g         1634 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tcataattca agttaataac cttaagggat accagttata
     1081 tttaaactaa attatagtaa tgaataattt tcttataata taaggtaaat taacaaaatg
     1141 gcttcgcatt taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac
     1201 attcgcttat atattgacca caactgatac atcagattat gtgatgactc gtgcttagat
     1261 caatttttgc aatcattaac aaaaagatta ataaaccatc tatatcaatt tatccaacct
     1321 attctgccgt tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg
     1381 actttcccgg acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt
     1441 tatccacaaa gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc
     1501 aaacataccc cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg
     1561 aaactattgc ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg
     1621 tgacaaagcg cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg
     1681 attgggcggc agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca
     1741 ttcagtttta tgacggtatc aagacctctg acatccacga aaccattatc aaggctgccg
     1801 cagacctgat ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct
     1861 tccacctgcg taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg
     1921 tgaaaatggt cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag
     1981 agttcaagca gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg
     2041 ccgttaagca gctggaaggc aaatatctgg tacagaaccg cgtgaccggc gaaatctatg
     2101 agagcgccca gttcctttat attctggttg ccgcgtgcct gttctcgaac tacccgcgtg
     2161 aaacgcgcct gcaatatgtg aagcgttttt acgacgcggt ttccacattt aaaatttcgc
     2221 tgccgacgcc aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac
     2281 tgatcgagtg cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgttaaat
     2341 acgtttccca gcgtgccggg atcggcatca acgccgggcg tattcgtgcg ctgggtagcc
     2401 cgattcgcgg tggtgaagcg ttccataccg gctgcattcc gttctacaaa catttccaga
     2461 cagcggtgaa atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc
     2521 cgatgtggca tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggtgtggaag
     2581 gcaaccgcgt gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgtc
     2641 tgctgaaagg tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg
     2701 cgttcttcgc cgatcaggaa gagtttgaac gtctgtatac caaatatgag aaagacgaca
     2761 gcatccgcaa gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg
     2821 cgtctaccgg tcgtatctat attcagaacg ttgaccactg caatacccat agcccgtttg
     2881 atccggccat cgcgccagtg cgtcagtcta acctgtgcct ggagatagcc ctgccgacca
     2941 aaccgctgaa cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt
     3001 tcaacctggg cgcaattaat aacctggatg aactggaaga gctggcaatt ctggcggttc
     3061 gtgcacttga cgcgctgctg gattatcagg attacccgat cccggccgcc aaacgtggag
     3121 cgatgggtcg tcgtacgctg ggtattggtg tgatcaactt cgcttactac ctggcgaagc
     3181 acggtaaacg ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcca
     3241 ttcagtatta cctgctgaaa gcctctaatg agctggcgaa agagcaaggc gcgtgcccgt
     3301 ggtttaacga aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc
     3361 tggataccat cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca
     3421 aaacgcacgg cctgcgtaac tctacgcttt ctgccctgat gccgtccgag acctcttcgc
     3481 agatctctaa cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt
     3541 cgaaagacgg tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg
     3601 agctgctgtg ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga
     3661 aatttatcga tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag
     3721 gaaaagtgcc gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca
     3781 aaacgctgta ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac
     3841 cgtcaatcca ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg
     3901 gatgcggcgt aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc
     3961 cagcgtcgca tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc
     4021 tcggatttgt aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa
     4081 aatgccttat ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc
     4141 acagacgaaa aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc
     4201 tcgctacgat cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt
     4261 cttctggcgt ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc
     4321 ggagcacgaa aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat
     4381 tcagggtcgt agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac
     4441 ctgggtcgaa acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat
     4501 tcgtaatatc gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat
     4561 ccagaaacgt gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta
     4621 ctggcacctt ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct
     4681 gcgcgagctg aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat
     4741 ccgcttctac gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg
     4801 taacgccaag attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca
     4861 gcatatgctg aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga
     4921 agaatgtaag caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg
     4981 ggcggattat ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca
     5041 gtacgttgaa tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca
     5101 gacgcgctcc aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt
     5161 tgctccgcag gaagtggaag tcagttctta tctggtcggg cagattgacg cggaagtgga
     5221 caccgacgat ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc
     5281 acacaactgc tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat
     5341 gttgcggttg agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc
     5401 gcaggtcagg tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg
     5461 ccctgttgtt gccgggcaaa aggcgatatt gaaatcgaga tgtgaattgg ttgtagtgcc
     5521 agatacaacg cttatacgtc ttatctggcc tacaacgatt acatggcgta gtaatacgct
     5581 ttcacctgct cccagtccgc tttggggatt ggctccagat attttccagc tggcggaagt
     5641 catgattaat cgctttattg cgacgcaagc ggcgacgact tttctccagg tcaagaaaac
     5701 cagcttctgc tttaccttct gttttcacat agatatggcg aacataacaa cagccatgct
     5761 gacgattaac gctatgcatt ttcttaaacg ccagcgccac tgctttcaac atggcttgcc
     5821 gtacttcgtc agaatagggc gatactgcat gccgggcata ccagtcagca atgctgatga
     5881 accccgccat atcttcagtc accagcaacg cgcgccattc accctcaatt ttcaccgctt
     5941 caccaaaaac gatcttcggc acgatgacac cggcccgttc cagttcttta attaccgcaa
     6001 cctcacggac aatcgttggt cggccgaacg gataacgtac ggaatgaaac agatgatgcg
     6061 tcatgcgctt tacatacagc tttttgccgt tgcgctcgac gcattgcacc ccgctcatac
     6121 cattacggcg atagttaggc tcttcaaccc agtcgccctc tgttgcccac cagtgattaa
     6181 attcgtcgta ctttgctgaa actgccatac cctatcgcct gtcattttta ttaacgacaa
     6241 tgactatagg tggttacctg aggaaaatct taatgaaacg tgtcgtatta atgaatttct
     6301 tttgccgcta ctagtgtgac accgcttttg cccttcattt gcagcgcata gattccacca
     6361 gccacgacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag gtggatacag
     6421 cgatgaattt tattcgacag ggattaggca tcgccttatt accagagtta acgctgaaaa
     6481 ccattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga ca
//
LOCUS       DAZPAC010000220         6512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZPAC010000220 DAZPAC010000000
VERSION     DAZPAC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.221"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            38..250
                     /locus_tag="ACHUPM_003681"
     CDS             38..250
                     /locus_tag="ACHUPM_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871351.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="ACHUPM_003682"
     CDS             283..501
                     /locus_tag="ACHUPM_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR0871352.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            572..889
                     /locus_tag="ACHUPM_003683"
                     /pseudo
     CDS             572..889
                     /locus_tag="ACHUPM_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHUPM_003684"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHUPM_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR0871353.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="ACHUPM_003685"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="ACHUPM_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="ACHUPM_003686"
     CDS             1917..2660
                     /locus_tag="ACHUPM_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR0871354.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="ACHUPM_003687"
     CDS             2690..3229
                     /locus_tag="ACHUPM_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR0871355.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="ACHUPM_003688"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="ACHUPM_003688"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR0871356.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHUPM_003689"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHUPM_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR0871357.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="ACHUPM_003690"
     CDS             4582..6102
                     /locus_tag="ACHUPM_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0871358.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..>6512)
                     /locus_tag="ACHUPM_003691"
     CDS             complement(6135..>6512)
                     /locus_tag="ACHUPM_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871359.1"
                     /translation="ANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKKN
                     TGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQA
                     SKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1712 a         1489 c         1550 g         1761 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gc
//
LOCUS       DAZPAC010000221         6441 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZPAC010000221 DAZPAC010000000
VERSION     DAZPAC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.222"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..331
                     /locus_tag="ACHUPM_003692"
     CDS             <1..331
                     /locus_tag="ACHUPM_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR0871360.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKQSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
     gene            complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHUPM_003693"
     CDS             complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHUPM_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR0871361.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHUPM_003694"
     CDS             complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHUPM_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR0871362.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHUPM_003695"
     CDS             complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHUPM_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR0871363.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHUPM_003696"
     CDS             complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHUPM_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR0871364.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4567..5001)
                     /gene="uspF"
                     /locus_tag="ACHUPM_003697"
     CDS             complement(4567..5001)
                     /gene="uspF"
                     /locus_tag="ACHUPM_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR0871365.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(5142..6275)
                     /gene="ompN"
                     /locus_tag="ACHUPM_003698"
     CDS             complement(5142..6275)
                     /gene="ompN"
                     /locus_tag="ACHUPM_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFR0871366.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT         1754 a         1517 c         1554 g         1616 t
ORIGIN      
        1 aattgaagca tggcgcacag aacaggaggc gaagccgttc acgtttgaat ggaacggtcg
       61 tatctggaat gctggcccca actcactggg ccgcctttat ccggtggtaa tggctgcgaa
      121 atccgacatt gtacgggacg tgatgacgtg gggtgatgcc gataatcagc aggtgaaaca
      181 gtcgatgccg gaactggaag aactggcggc agcaatggct caggcacagg ttgagcgcaa
      241 tgacgagatt tatcgccgtc agcgggagct gaaggaagag ctgaatagtc tgaaggattt
      301 gaattcggtt aggaatttta tcgtggaata acagaagctg cggcacgtcg tatgcaggaa
      361 cgtgccgcgg ttggctggta aactttcgat agtgcgagta ttgaatgatt tctaggcgtt
      421 atcgatttta cgtatttttt gcatgataga attcgtacct cctcccaacg accttccatg
      481 gctttccgga cttctgtagg ctggcggacg atgctgtcct cggatgttgt gctaattggg
      541 tgataacaga tcctgtgtat gggcgtttcc ctttgtgcgt gcgttgatgc tggtaacggt
      601 aaacgtcatg atgaacacga ccgcgaataa gacgacaatc gtcggagctg gtgcgctgtc
      661 gataaaaaag gacagccaga caccagtcat tgacactatt accgatatgc cagtcgccag
      721 taggagcgca atatgaaagc gttgtgtcag gagtactgca atggcgccag gcgcaatgag
      781 cagagaaata gagagaatga tacctactgc tttcagcgtc gccacaatgg tcagggaaac
      841 catgcacagc agcccataat gcagccatct cgtatgcaaa ccgcttacct gcgcctgttg
      901 ataatcaaag ctgaacagca aaaaatcccg ccattttgcg ctaataacaa gtgtaaccag
      961 cccggcaata atcaccgtct ggattatatc gccgatggtt atcccgagca tgtcgccgaa
     1021 aagaatatgg tcaagatgta cgtctggttt gattgcaata tacaagatca gacctgcggc
     1081 aaacatgcca gaaaaaacaa tccccataac tgtatcttgc ttgattcggc tgttgtcttt
     1141 caggtatccc gtcgccaccg cgcagaatac tccggcgacg aatgcccctg tagccagagg
     1201 taaccccaga atccaggtaa gcacgatgcc aggaaaaacc gcatgactca tcgcgtcacc
     1261 catcagtgcc caacctttca ggaccagaaa gaccgacagc agcgcacagg gtatggaaac
     1321 taccagtgaa atcagcagtg cgtgactcat aaaggtgaac tgcagtggct ccagtaataa
     1381 tgccatcatg agcgatcctc cagcgcttta tgcgcacgcc gacgatttgc caaaagtcca
     1441 tgggtgggcg caaatacaaa tgctaaaaga aacagcagtg tctgcgccac cacgatgatt
     1501 ccacctgtcg cgccgtccag aaagtaactg acccacgcgc cgaggaaact ggttacactg
     1561 ccaatagtta cagcaattat gagcaggcgg ggaaaacgat cggttaacaa ccatgctgtt
     1621 gcaccgggag tcaccaccag acagatcacc agaaaggcac caaccgtttg cagcgccgca
     1681 acggtcgaaa ccgacagcag ggtgaagaag aggattttta atctgcccgg atgtaatcca
     1741 atagcacggg cgtgattctc gtcgaaaaag gtcaccataa gatctttcca tttgaaaaaa
     1801 agcacgatga tcgagagaat gccaatgata gtgagttgca ggatatccgc cggatcgatt
     1861 gccagaatat tgcccaggac gatggtctga atgttcacgg atgtcgggtt caatgacacc
     1921 ataaacagcc ccaggccaaa aaaggacgag aagatcaggc cgatgatggc atcttctttt
     1981 aagcgggtgc gttggttaag aaacagcatg ctgcccgctg ccagtccgcc ggaaaagaag
     2041 gctcccagag aaaacggaag ccccagcata tatgcccccg caacacccgg gacaatagag
     2101 tgcgacagcg catcgccaat cagcgaccag ccttttagca tcagatagca tgacaggaaa
     2161 gcgcagagac cgccgaccat tgccgagacc cacatcgcat tgagcatata ttcatagctg
     2221 aagggttcca gtagcacgtt catcttgctt ccctctgcac cgctgacggt cgatgtgcca
     2281 caaaagggcg ttcatcgtca gtaataatgc tttcttccga gtcgttaagt gtgacatggc
     2341 gcagtacgcc gctaaaagcc agctccaggt tctcggctgt aaaagtggtg tctgtcggcc
     2401 cgcttgccag gacggtgcct ttgaccatga ccgtataatc gcaaaatgtc gtgactgagc
     2461 cgagattgtg cgttgagaca agcatcgttt tgccttccgc gcgtaactcc ctcaacaggc
     2521 tgatgatttt cgcttcggtt ttcacatcaa cgccggtaaa aggttcatcc agcaggatca
     2581 catcaccctg ctgtgcaatc gctctcgcca gaaagacgcg tttcttttgt ccaccggaaa
     2641 gctcgccgat ttgacgatgg cgaaaatcca ccatatcgac acgttccagc gcatcggtga
     2701 caatctgacg atccctcttt ttggctatac gcaacatacc catatgccca tagcggccca
     2761 tcatcaccac atcttccacc agtacaggaa atgaccagtc aacctcttct gactgaggga
     2821 cgtaggcaac caggtttttc tgtagcgcct gtcgcgtggg aatacccaga acagatatct
     2881 ttccgctggt cagacgcaca aatcccataa tcgctttaaa caacgtcgat tttccggaac
     2941 cgtttacccc aaccagagcg gcaatagatc cacttggtac tgtgaaggat gcatcgcgca
     3001 gtgctgtgtg cccgttacgc caggtgacag taacatcatt aacgacaatg cctgcagatt
     3061 gcatcattat ttctcccttt tcccggcttt aattccctgt accagcgtac tggtagtaac
     3121 cttaagaagg tcgatatacg taggtaccgg gccgttttct gtgctcaggg aatcgacata
     3181 gagcacaccg ccgtagtgtg cgccggtttc acgcgcaacc tgacgcgctg gtttatcgga
     3241 aatcgtactc tcgctaaaga ctgccgggat atgatttttc ttaactatat caacaacctt
     3301 acgtacctgc tgcggtgttc cttgttgatc ggcattaatc ggccacagat aaagctcttt
     3361 tagccccaaa tcgcgtgcga gataagaaaa agccccttca ctggtgacca tccatcgctg
     3421 attctcaggg agttccgtaa tctgcttacg caggggggca agggtttggg taatcttggc
     3481 tttataagta tcggcattac gttggtaggt ttgtgcattt gccggatcgt attttatcaa
     3541 cgcatcacga atattatcga cgtaaatcag agcattatct ggcgacatcc atgcatgggg
     3601 attaggtttg ccctcatagg gtccttcggt gatccctact ggcgtcacac ccgaagagac
     3661 aattacttct ggaaccccat tgagatgctg gtaaaagcgt tggaaccaca attccagatt
     3721 cataccattg gcgagaatca gttgtgcccc ctgcgcacgt ttaatatcgc caggggtagg
     3781 ctgatactca tgaatttctg caccaggctt ggttatggat gagacttctg cagcatctcc
     3841 agccacgttt ttggccatat ccgcgatgat ggtaaatgtt gtaatgacct tgaatttttc
     3901 agttgcactt gcctgaaatg cgatcgagca ggtgagggcg aggcacccca agagcatggt
     3961 tacttttttt attgagtgca taatttagta cctatagtga gcaaaattag aacaaacata
     4021 gcaacggcta tgttttatag cataacccat acaaaatgaa aatgattatc attaacagct
     4081 ttgaggccta aaacagggat taggttgttt cctgctggcg acatgatatt gaggaaaaaa
     4141 gtagagcagc tattaggctt tgaaatgact ttatgaggaa aattgttgat ggcaaaaatg
     4201 gggcaaaacg ctgtagaagg ggcaaaaaag gggcaaaaaa agagtgggtt atcgtagctt
     4261 attgttgtcg ctgataatgt ttaacatatt gaaaaataaa taaaatactt atgggtcaga
     4321 tagttgtgat ttttgccctt acttgttcag gttgtattgt tctttcttac taatttcttg
     4381 attttgcgac atttaaaagc gactcaattc gttatatggc atcagaagag tatgcgtcat
     4441 gccggaacgc ccagcataag aaatctgata taaaactgtg gcgtgtatgg tacggattag
     4501 aggggaaaat gtcagcacat ttgcgaaatg aatcaaaaag cccgcagcaa tgtgcgggcg
     4561 ttagtgtcag cgcacaacca gcacggagca ctctgcgtga cgcactacag ctgcggcgtt
     4621 ggaaccgagc agataagtgg tgatatccgg tcgatgggaa gcaatgatga tcatatgagc
     4681 ggggatcttc ttcgccaatt ccagaatgcg gtctttgggc gagccttcct caacatggac
     4741 atgcactctg tcggttggca gtttaaattt tttaatgatc tcttccagtt gcgatttggc
     4801 ttccgctttc aggtcatcca ttgccggtaa ttctgcggaa tacgctaaac ccagagaggc
     4861 atagtaaggc agtgaaggta ttaccgtcag gaaatgaacc tctgcatcgt caatctttgc
     4921 ctctgcctca acgtggctaa tcacgcgttg agttaattct gaatcggaaa tatcgatagg
     4981 gacaagaatc gttctgttca taaaacctcc tgttttagta tccgcataaa gtgtaacgcc
     5041 agatgacact ttttgtgtaa tgacggagtt cacattttta atttagatca aaggaggaag
     5101 aataagcaga aaaagcccgc cataacagcg ggcaggagga tttagaactg ataaaccaga
     5161 cctaaagcga caatatcatc ggtagagatg ccgttggcag cgtagaaact gtcatcttca
     5221 tccaacaggt tgattttata gtcaacataa gtggacatgt ttttattgaa atagtaagtc
     5281 gcgccaacat cggcgtattt aacaagatct ttatcatcaa cacctgccgg gttgtctgca
     5341 ccatcggcag cgtgcaggtc acggccttta gacatcagga aagagactgc cggacgcaga
     5401 ccaaaatcaa actggtactg tgcagtgact tcaaagttct gggttttgtt tgcaacagca
     5461 taatcgctgt cgccaaacgg ggtcatatta cgcgtttctg aatacatggt tgccaggtaa
     5521 atattgttag catcgtattt tagcccagca gtccacgcgt ctgctttatc accacccgcc
     5581 gcagtatggt taacctggtc attggtgcgg tcagatgagg tgtatgccgc accagcgcta
     5641 aagcccatgc ctaaatcata tgttgtggaa agaccccagc cgtcaccatt ttcatggcga
     5701 acatcacgtc cgttgttggt gccttcctga ccattactgg caccttcgtt gttaccttga
     5761 tactgcaccg cgaagttcag accatttacc agaccgaaga aatcagtatt acgataagtc
     5821 gcgacgccgt tggcacgacc agtcataaag ttgtctgcat tggtataaga gtcaccgcca
     5881 aattcaggca gcatatcggt ccagccttcg atgtcgtaca ttacgccata attacgtccg
     5941 taatcgaaag aaccgtaatc tgcaaatttc agcccggcaa atgccagacg ggtccatgac
     6001 tggttttttg aagattcagt gttgtttgcc tgaatattgt attcccattg accgtagcca
     6061 gtgagttgat cgttaatttg ggtttcgcct ttaaaaccca gacgcgcata gctctgatcg
     6121 ccatctttcg ctgaattatc agaaaaataa tgcaggccat caactttgcc atacagatct
     6181 aatttgttgc cgtctttatt ataaacttcg gctgcatgtg cagcacctgc ggcgagcagg
     6241 gcaggaatta aaagtgccag tactttgctt ttcattgaat aaatccttta gttattttat
     6301 ttgcctttta tccccattcc ggggagtgaa aacatcctaa aggagaagtt cagttagccg
     6361 ataaatatca tttgttacgc cttaagtaaa accttaatca aataattctt ctgaaatgat
     6421 aataagtctg gtgaatgtat c
//
LOCUS       DAZPAC010000222         6400 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZPAC010000222 DAZPAC010000000
VERSION     DAZPAC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.223"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUPM_003699"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUPM_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0871367.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHUPM_003700"
     CDS             2219..2767
                     /locus_tag="ACHUPM_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR0871368.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUPM_003701"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUPM_003701"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR0871369.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHUPM_003702"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHUPM_003702"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR0871370.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHUPM_003703"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHUPM_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR0871371.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1615 a         1567 c         1616 g         1602 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcagtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt
//
LOCUS       DAZPAC010000223         6328 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZPAC010000223 DAZPAC010000000
VERSION     DAZPAC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.224"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(122..898)
                     /locus_tag="ACHUPM_003704"
     CDS             complement(122..898)
                     /locus_tag="ACHUPM_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871372.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(949..1383)
                     /locus_tag="ACHUPM_003705"
     CDS             complement(949..1383)
                     /locus_tag="ACHUPM_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR0871373.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(1397..1618)
                     /locus_tag="ACHUPM_003706"
     CDS             complement(1397..1618)
                     /locus_tag="ACHUPM_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871374.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(1619..2302)
                     /locus_tag="ACHUPM_003707"
     CDS             complement(1619..2302)
                     /locus_tag="ACHUPM_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR0871375.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2379..2606)
                     /locus_tag="ACHUPM_003708"
     CDS             complement(2379..2606)
                     /locus_tag="ACHUPM_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871376.1"
                     /translation="MTRRGRNAACGQCPASGRARDGRFYRSRLALVTVYASPSPFSDE
                     RPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            2507..2713
                     /locus_tag="ACHUPM_003709"
     CDS             2507..2713
                     /locus_tag="ACHUPM_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871377.1"
                     /translation="MSPTPAGSGKSARPVPGRTRDTDHRQRSCRAASAGAVKRGARRH
                     LLLPAKQNPKTTHTQPNKTPKQDK"
     gene            2819..3781
                     /gene="parM"
                     /locus_tag="ACHUPM_003710"
                     /gene_synonym="stbA"
     CDS             2819..3781
                     /gene="parM"
                     /locus_tag="ACHUPM_003710"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR0871378.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            3784..4134
                     /locus_tag="ACHUPM_003711"
     CDS             3784..4134
                     /locus_tag="ACHUPM_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR0871379.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(4257..4538)
                     /locus_tag="ACHUPM_003712"
     CDS             complement(4257..4538)
                     /locus_tag="ACHUPM_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871380.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(4666..4900)
                     /locus_tag="ACHUPM_003713"
                     /pseudo
     CDS             complement(4666..4900)
                     /locus_tag="ACHUPM_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637348.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5836..>6328)
                     /locus_tag="ACHUPM_003714"
     CDS             complement(5836..>6328)
                     /locus_tag="ACHUPM_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871381.1"
                     /translation="GLFILSGENKIDEECFINIVQTAVAINNIGRESNGYILVGVSDT
                     KATADRVKTLYGVTPIECNGYYINGIDHEAVIQSKNIDNYFLFIKQKIESFNFNEALK
                     QQILKDISLCSYEGLHVLKIEIKSVGEVCHFENRYYIRQGTATHLITDGEAISSLHRL
                     YYS"
BASE COUNT         1706 a         1576 c         1429 g         1617 t
ORIGIN      
        1 aactgaaaaa ccacaaggcc atgccctgca tggccgggta aagcggagcc ggcggcgact
       61 tcctttgtgc tctgtccggg gaccggcagg aaaaaggcgg ggatttaccc cgccaacgtc
      121 atcatttccg gatttttgcc agggcatccg ccatgctgcg gatttcggcc cgggcttccc
      181 ggaccacctc ccgcagaaca ccacggtcaa accagcggcg gggcggttca tccggtatac
      241 gcagcacatc gcaggccagc ccactggaca ggatatgctc aatacaatcg ctgaccaggt
      301 acatttccac cgacgaaaaa cgtgctcccc ggttcacgat ggcatcgagc accacgggca
      361 ggttttcgtc atcttcatcg agccagcagg ggtgatcgct ttccttcaca accagacgaa
      421 agcggcgcgt ctggcgagtc agcaacgtct gaccggtggt ggtcagcgcc gcccggatac
      481 gccgcgcatc ttctgccacc cggcaactga cactgcgtac cgcatcgcgc accgccgcaa
      541 gtacagccct gatttcgtct tcggtcagcc attctccggc atacgggaaa gacacctgcg
      601 ttccgtctgc cggaagtacc ggcagcgact gccactgact gccggacagt accagttccg
      661 ccccggttcc ggtcagtttc tgaatggcgg aggccaggag cggcggcagt acgccgccgt
      721 gacggttgat aagtaacgtc gccagccggg gcgcatccag cagaacataa atgctgatcc
      781 agcccggata cttcgggtta cgctggtcaa catccgtctc ttccggccag ccctgcgcca
      841 gcagcaggtt gcgggcgtga cgacaaatca gtgcgttcag cgtgctgttt tcgttcatga
      901 ttaagcgttc tccctgtatc gccgcccgca gggcaggcgg cgataacatc agtcgttttc
      961 gtgtgtgttg tttttcagca gggtgcgggc ctgattaacc acctcctgac ggcgggtgac
     1021 gcattccggg acggcgaggc catgatcccg ggcggcccat tcacgtttcc acaatgcctg
     1081 ttcagcggtc tgaatcaggg acgccagcag ttcagcaggg acggtcacca ggcggttttc
     1141 gctctcgttg cggataatct ccatcaccgc agcggaagaa ataccggact caatccgctg
     1201 gtcttccggg atgtcctcca ggcgtgccag gacggtcctg atttcatgac cggtcagaga
     1261 aatttccata ccgtcagcca gtgcctgaat ctcttccggg gaccagataa ccagcatcag
     1321 cggcatgtct cccggatact ttactgccag ctcccggcat agcgtttcgc atgttccgta
     1381 cattctcgtc tccttctcaa tctttaaagc acgcttccag ttccagaatt tcggtatatg
     1441 cctccatcag gaggcggttt gcgcgaaaca gcgtctgacg caccagacgt tccggcaggg
     1501 tgtcggcatt aaagctgtac cgctgctcca tctcattcat ggttgtacgc aggtcgcaca
     1561 tggcattggc cacctccgcc acttcggcgc gcagtttttc gtgtcctgca tagttcattt
     1621 acgcagcctc cggcataaac cagtcatcat tcgcggcccc ctgctgcatg gcccgttgca
     1681 cggcggcaag gcgttgctgc ccggcacggt gatactgctc aagcagctcg ataccgatat
     1741 accggcgtcc ggactggagg gcggcgacgc aggttgagcc gctgcctgca aacgggtcca
     1801 gcacaattgc gttcgggtgt gtgaagctct caatcagcgg ttgcaggctg gtaacgggct
     1861 tttccgtcgg gtgatggcga ttgcccgaat atttccagcc cagcacgtcc ggcagcgggt
     1921 tttgtggcag acgtggacgg ccttttgcca ggatgtaggc acattcgtgg cgatagccca
     1981 catatgcggc cttcgatgtg tagtttttgg tgaagaccag gtgaccaaca acgctgaatc
     2041 ccgcattttt ccaggcggcc ataaagcgat cgacgcggtt ccagccatag aagctcacca
     2101 ttaacgcgtc ttttttgagt acgcggtaca tttcattaca ggctggttgc agccattcat
     2161 cggttttatc gccggcgatg gtgcgcccgg aacggtcacg gaaaccgacg agatacggcg
     2221 ggtcggtgag gatgaaatcg acagcattgc ccggaaacgt ggccataaca cgcacgcagt
     2281 cacccaggat aaaacgggac ataacagcac tccttctctt acagagggca ccgggagggc
     2341 acccctgtgg tgcccgttcc ccgctgcctg tgggctgttc accgcgtccg gtaacgggtc
     2401 aggccgaccg tggaataccg cagccgtcgt ctttttgacg gcgaggatat gccgcgaaag
     2461 cggcttgaag ggcgttcatc agaaaagggc gacgggctgg cgtacactgt caccaacgcc
     2521 agccgggagc ggtaaaagcg cccgtcccgt gcccggccgg acgcgggaca ctgaccacag
     2581 gcagcgttcc tgccgcgccg cgtcagccgg cgcagtaaaa agaggagccc ggcgacatct
     2641 tttgttaccc gccaaacaaa acccaaaaac aacccatacc caacccaata aaacaccaaa
     2701 acaagacaaa taatcattga ttgatggttg aaatggggta aacttgacaa acaaacccac
     2761 ttaaaaccca aaacataccc aaacacacac caaaaaaaca ccataaggag ttttataaat
     2821 gttggtattc attgatgacg gttcaacaaa catcaaacta cagtggcagg aaagcgacgg
     2881 aacaattaaa cagcacatta gcccgaacag cttcaaacgc gagtgggcag tctcttttgg
     2941 tgataaaaag gtctttaact acacactgaa cggcgaacag tattcatttg atccaatcag
     3001 cccggatgct gtagtcacaa ccaatatcgc atggcaatac agcgacgtta atgtcgttgc
     3061 agtgcatcac gccttactga ccagtggtct gccggtaagc gaagtggata ttgtttgcac
     3121 acttcctctg acagagtatt acgacagaaa taaccaaccc aatacggaaa atattgagcg
     3181 taagaaagca aacttccgga aaaaaattac attaaatggc ggggatacat tcacaataaa
     3241 agatgtaaaa gtcatgcctg aatctatacc ggcaggttat gaagttctac aagaactgga
     3301 tgagttagat tctttattaa ttatagatct cgggggcacc acattagata tttctcaggt
     3361 aatggggaaa ttatcgggga tcagtaaaat atacggagac tcatctcttg gtgtctctct
     3421 ggttacatct gcagtaaaag atgccctttc tcttgcgaga acaaaaggaa gtagctatct
     3481 tgctgacgat ataatcattc acagaaaaga taataactat ctgaagcaac gaattaatga
     3541 tgagaacaaa atatcaatag tcaccgaagc aatgaatgaa gcacttcgta aacttgagca
     3601 acgtgtatta aatacgctca atgaattttc tggttatact catgttatgg ttataggcgg
     3661 tggcgcagaa ttaatatgcg atgcagtaaa aaaacacaca cagattcgtg atgaacgttt
     3721 tttcaaaacc aataactctc aatatgattt agttaacggt atgtatctca taggtaatta
     3781 atgatggaca agcgcagaac cattgccttc aaactaaatc cagatgtaaa tcaaacagat
     3841 aaaattgttt gtgatacact ggacagtatc ccgcaagggg aacgaagccg ccttaaccgg
     3901 gccgcactga cggcaggtct ggccttatac agacaagatc cccggacccc tttcctttta
     3961 tgtgagctgc tgacgaaaga aaccacattt tcagatatcg tgaatatatt gagatcgcta
     4021 tttccaaaag agatggccga ttttaattct tcaatagtca ctcaatcctc ttcacaacaa
     4081 gagcaaaaaa gtgatgaaga gaccaaaaaa aatgcgatga agctaataaa ttaattcaat
     4141 tattattgag ttccctttat ccactatcag gctggataaa gggaactcaa tcaagttatt
     4201 ttcttaccag tcattacata atcgttatta tgaaataatc gtttgcactg tctctgttat
     4261 tcaggcaatt tcaataaagg cacttgctca cgctctgtca ttttctgaaa ctcttcatgc
     4321 tgcatttcga tttttcttct aaaatcataa cttgcatacc tgttcttttt atgtatatcc
     4381 agatctattt ttgtgacgag ttcaaaccac aatgatagca ctccccatgc agcgttatga
     4441 aacatttccg caacagattt agagtctgta ttaaccgctt cagtcatgta gtcgctaata
     4501 cgttctgcag tgattttcgt tatttcatcg taattcattt tatcccctac aagactattt
     4561 aatggaatga aaaatatttg gtctgataac aatgctcctg aattaacacc tgcaggctat
     4621 gttaaagttg ccctcacaca caatctacct gttttctcgt gtgtgtcaca ggcgcaatac
     4681 ataaatagtc tggccattaa gttcgtaacg gatttcgtac tcgccagcaa aaatccgtct
     4741 gacatccctg gtttcaaact gaaacaactg ttcatcccat acgtggatga gttaacagaa
     4801 tgaccggcgc ctgtgtcagg gactacaccg ttttggctgc agctggttta ctgaccaacg
     4861 ccagaaaatc atataactgc gtcaatgtca gcgtatccag cgcgactgta ttatttttct
     4921 ctgcaaccag aatattgcat taggttatta ccaggtgttc taatgtacag gtaaagacgt
     4981 tctttataat ctgaaatctc tacttttaat ccctcagact ttgcctgctg aaagccctcg
     5041 tcaaggtgcg cttttattag gttaatgtct ggatgtcttg tatcgggggc aattctgtat
     5101 tggtgtaacg atggggtgga tgttccctgc aataagacct ccagatagag attggcacaa
     5161 ccattcccat tatttctaat agtttattgg taatcacaaa ctgtatattt agccattata
     5221 ttgaatcctg tgaagtaaac ggcgacggct caaaatttta tcaaacctaa gataattcta
     5281 ccatcaaagt taataatttg aatttatatg acggctctat gggggggtaa aaatccccgt
     5341 gcagagaggc gattttcaga gttgaaaacg tcgtcgttaa gaacaccaaa gtcagtacca
     5401 ccaacccgga aagtagcttc atgcaccggg acaacagcag ttttggtgtc cgaaatcgta
     5461 caattctgta catctacgca aggaaatgta cggaagtaag ccaaatggtt atcggacggt
     5521 cggaacattt cgtacagcct ttacgttcag tgagttagct ggcctagatg taaccactcg
     5581 ttcaactcaa ttctcttagc gtgggaaaat ttccgttttc caagcggccc cctcaagcgg
     5641 accccttata gtacaataat tttcgatatc caaactgccc ccaattacat ctccagtctc
     5701 tggcagcaga gatcatatat ttccattgcc gccgtagcca cgatattctg gctattcaca
     5761 aaaacaattt agccccaaag cgcagtaaaa aagtacacta tgaaggtgta tgataccttc
     5821 atagtgctta agtaattatg agtaatataa tctgtgtaaa ctactaattg cttcaccgtc
     5881 agtgattaaa tgcgtagctg ttccttgtct tatataatac ctattttcaa aatgacagac
     5941 ttctccgaca ctttttattt cgatttttaa aacatgcagt ccttcatatg aacataatga
     6001 aatatctttt agtatttgtt gtttcaaagc ctcattaaaa ttaaatgatt ctattttttg
     6061 ttttatgaac agaaagtaat tatctatgtt ttttgattga attacagctt catgatcaat
     6121 tccattgata taatagccat tgcactcaat aggggtaaca ccatataaag tcttaactct
     6181 gtctgccgta gctttggtat cggaaacacc aactaaaata taaccattag attctctacc
     6241 gatgttattt atggcgacag ccgtttggac tatattaatg aaacattctt cgtctatttt
     6301 attctcgcct gagagaataa ataatccc
//
LOCUS       DAZPAC010000224         6325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZPAC010000224 DAZPAC010000000
VERSION     DAZPAC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.225"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..355
                     /gene="mcbR"
                     /locus_tag="ACHUPM_003715"
     CDS             <1..355
                     /gene="mcbR"
                     /locus_tag="ACHUPM_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR0871382.1"
                     /translation="TPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNM
                     PILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAE
                     IMQQNIAILYQQYNR"
     gene            complement(391..2493)
                     /gene="pqqU"
                     /locus_tag="ACHUPM_003716"
     CDS             complement(391..2493)
                     /gene="pqqU"
                     /locus_tag="ACHUPM_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFR0871383.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2735..3796
                     /locus_tag="ACHUPM_003717"
     CDS             2735..3796
                     /locus_tag="ACHUPM_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFR0871384.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3911..5410)
                     /gene="ansP"
                     /locus_tag="ACHUPM_003718"
     CDS             complement(3911..5410)
                     /gene="ansP"
                     /locus_tag="ACHUPM_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFR0871385.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5677..6294
                     /locus_tag="ACHUPM_003719"
     CDS             5677..6294
                     /locus_tag="ACHUPM_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR0871386.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1704 a         1594 c         1513 g         1514 t
ORIGIN      
        1 caccccgcaa gacctgacgg aactccagga attgctcgag aaattacagc aggcgcaaga
       61 aaagggcgac atggaacaaa tcattaatgt aaacaggcta tttcgcttag cgatttatca
      121 tcgctcaaat atgcccatcc tgtgtgagat gattgagcaa ctgtgggtca ggatggggcc
      181 tggtttacat tatctctatg aagcgattaa tccagcggaa ttacgggagc atatagaaaa
      241 ctatcatctg ttactcgctg cgttaaaagc aaaagacaaa gagggatgca gacattgtct
      301 tgctgaaatt atgcaacaaa atattgctat tttataccag caatacaatc gttaaagtaa
      361 atgcccggca aataccgggc atcaaggtaa ttactcaaat ctccacgcaa tattcatgcc
      421 gacaccataa tttcgtccgg gcgaaggttc gtaatatcgc ccgtttgact cattgacaat
      481 gacagaacca acgtattctt tatcgaataa attatcgaca cgaccaaata aatccacagt
      541 taaattgtgg taattgtatt tatacccggt gaataagccg acgagagtat aagacggcgc
      601 ttttgccgta ttttcatcat ctgccataat atcgcccata taacgcgctt ccgtgcctgc
      661 ataccaacca tcttccggta cataacctat cgacgcaaag cccatattac gggcgatccc
      721 tggcatccga ttaccgttac aatcctgttc attgcaaaca ttgctgcgat aggtcgcatc
      781 aagccaggtc caggacgcgt ttacgcgaaa atctcctgcg aaacgttgat cccatgccag
      841 ttcagcgcct tgacgacggg tctttccggc atttttgtaa gtcgtacgcc caccgctact
      901 gctatcgaca acaatttcat catcagtgtc ggtctgaaac aatgcgagac taagcagccc
      961 atcaccaata cgcgttttac tgccgatctc aattgtatcg ttggtggatg gttttaaacc
     1021 aaagttcata ccgctttgcc catcagcacg ataagacagc tcattaatcg tcggcgtttc
     1081 aaaacctcgc ccggctgcca gatagatatt ccaggcatcg gtcattgcat attttaacga
     1141 accggcaggt agccatttat gataactggc atcaccgctg tcatcgccgt tacccggagt
     1201 aacgtaatgg tcgttggaat caaaccacac ggagctgtag cgcacgccag catccagcga
     1261 cagtttttcg ctcagctgcc actgcgtctg taaataggga tcgatgttcc acatcagatt
     1321 gcgttcgtcg cgacgcaact cacctttttg cccgtactcc ggcatgccgc tattcaggcg
     1381 gaagttattg tagcccttgc ggttttcact catgttttcg tagttcaggc cggtagtgaa
     1441 cgtgaccgga acgcccagtt caccacggtg tgtccagcgg ctgtctatcc cctggtaatg
     1501 gcgttgcagg gtaatcacgc cgcccgcatg tgacgggtta agttgtggtg ccatgggtat
     1561 tgactggtac tgggtcgttt ctcgctctcc ggcatacatc atcacactca tatcatcttg
     1621 cgcgctcagg ctacgctcat agcgcaaccc agcctgagtt tgcttgatgg tttttcgcgt
     1681 gtcgtactgt tctgcacgag gcgcttgttg tggattagcc ttccattctg ctttggttag
     1741 cccacccggg tcatctgctt tgatatccac actattgaaa atcagactta atttgctggc
     1801 ttcatcaatg cgtacgccca gtttggcatt ggctaaattt ttctgtgcgc cactatggtc
     1861 acgatagccg tgggtcgtaa aacgcgtggt tgagacggtg taatcgacat cgccaggctg
     1921 tgtgccgtct cccgttgcgc ccgttgcttt cagcccatag cgccagctgc caaaactgcc
     1981 gtagtaacta ctggcttcaa tggttggtgg ctgttgtccg gtctgggtgg tgacattcat
     2041 taccccacca gacgcgttgc catacagggc agagaagggg ccacgcagca cttccacatt
     2101 ttgcacactg cttaaatcga tgttggatgt ttgcccttgc ccgtcgggca tggtggcggg
     2161 aataccgtcc acatacaggc gaataccgcg aataccgtaa gtggagcggg agccaaatcc
     2221 gcgaatcgac agctgtaaat cttgcgcata gttctgccgg ttttgtacct gcaaaccagg
     2281 cacgtcggtc agtgattcgg acaagttaat gcgcggtgtt gccaggcgca tctcctcgcc
     2341 atccaccacg cttactgctg ctggggtatc cagttctgaa accacctgcg gtgcggcact
     2401 gacaatcatg gtctgttcat cagcggcaaa aacaacgggg gaaagggcaa gtagtgcggg
     2461 caaaacggtc tgtcggacgg aaaaaatctt catgaaaaaa gccaggttaa gaatgggaaa
     2521 acgccgttat ggtaataaaa ttgtaaattc atggaaaacg aaacggcacg atacgttaag
     2581 taattgagaa aattgtagtc gtaacggcaa gaaatgctcc acatttgaga aaataatgat
     2641 taccattccc atttataaca agagcgtaac gatgattacg cttagcgaag cattgtgaag
     2701 cagcaaaaat atcggttcat caaagggagt cgtcatgcat ttacgtcatc tgttttcatc
     2761 gcgcctgcgt ggttcattac tgttaggttc attgcttgtt gcttcatcat tcagtacgca
     2821 ggccgcagaa gaaatgctgc gtaaagcggt aggtaaaggt gcctacgaaa tggcttatag
     2881 ccagcaagaa aacgcgctgt ggctcgccac ttcgcaaagc cgcaaactgg ataaaggcgg
     2941 cgtggtttat cgtcttgatc cggtcactct ggaagtgacg caggcgatcc ataacgatct
     3001 caagccgttt ggtgtcacca tcaataacac gactcagaca ctgtggtttg gtaacactgt
     3061 aaacagcgcg gtcacggcga tagatgccaa aacgggcgag gtgaaaggcc gtctggtgct
     3121 ggatgatcgt aagcgtacgg aagaggtgcg cccgctgcaa ccgcgtgagc tggtagctga
     3181 cgatgccacg aacaccgttt acatcagtgg tattggtaaa gagagcgtga tttgggtcgt
     3241 tgatggcgag aatatcaaac tgaaaaccgc catccagaac accggtaaaa tgagtaccgg
     3301 tctggcgctt gatagcaaag gcaaacgtct ttacaccact aacgctgacg gcgaattgat
     3361 taccatcgac accgccgaca ataaaatcct cagccgtaaa aagctgctgg atgacggcaa
     3421 agagcacttc tttatcaaca ttagccttga taccgctagg cagcgtgcat ttatcaccga
     3481 ttctaaagcc gcagaagtgt tagtggtcga tacccgtaat ggcaatattc tggcgaaggt
     3541 tgcagcaccg gaatcactgg ctgtgctgtt taacccagcg cgtaatgaag cctacgtaac
     3601 gcatcgtcag gcaggtaaag tcagtgtgat tgacgcgaaa agctataaag tagtgaaaac
     3661 gttcgatacg ccgactcatc caaacagcct ggcgctgtct gccgatggca aaacgctgta
     3721 tgtcagtgtg aaacaaaaat ccactaaaca gcaggaagct acccagccag acgatgtgat
     3781 tcgtattgcg ctgtaataga tatggcgagg gtgaaaacgg agagacttgc ctgactttta
     3841 tcacgccgca tgcggcacac cttcgtcagt aatctaaatt tgcccggcac gtagccgggc
     3901 aattttgcaa ttagctcgcc gtttcaggct taaacacaat ttcctgtttt tcttcatctt
     3961 cctcgacgac tggcgcagtg ctgtgaattt cagcaacgcg tttgcgcaca ccaaaccagc
     4021 ctataaccag cagaataccg ataatcggca gcgccgcgat agtataagtc ccgttcgggt
     4081 aatcgaacgc catcagcaca aggacactca gtaaaaacag taatgtcagc caggaagtga
     4141 agggcgcgcc aggcagttta aaactgacat ccgctgcttt gccttcttta atcgctttac
     4201 gcaggcgcat ctggcacacg atgataaacg cccatgaagc gatgattccc agcgacgcga
     4261 agttcaacac aatctcaaat acgcgcgacg gcaccagata gttgaggaat acgccgacga
     4321 catacacaac cagtgtcgcc agaatcccgg catacggcac atgctgacga ctcattttcg
     4381 ccataaaact cggtgcggaa ccgcccatcg ccatcgagcg cagaatacgc ccggtgcagt
     4441 acagacctga gttcaggctg gagagggcag cggtcagcac cacaatgttc ataatgctgc
     4501 cgatatatgg cacacccagt ttagagaaaa acgtcacgaa cggactttgc cccgcctgat
     4561 acgcgctcca cggcaataac ataaccagca acaccacgga gccgacgtaa aacaggccaa
     4621 tacgccaaat cacactgtta atggctttag gcaccatggt ctgcggatct ttacattctc
     4681 cggcagctgt acccaccatt tcaatggagg caaaagcaaa cactacgccc tgaatcaaca
     4741 ccagcgcagg cagcaaaccg tgggggaaga agccgccatt atcggtgatt aaatgaaagc
     4801 cagtggtgtt gccatccagc ggctgaccac taccgaggaa cactgtaccc acgaccaaaa
     4861 aggtcacaat ggcgagcact ttaataagcg caaaccagaa ctccatctcc gcaaaccatt
     4921 tcacaccgat catattcatg gtgccaacga tggtaagtgc agcgagcgca aagacccact
     4981 gcggcacgcc gccaaacgca ccccagtaat gcatatacag agcgacggcg gtaatatcaa
     5041 caatccccgt catcgcccag ttgatgaagt acatccagcc agcaacataa gcggctttct
     5101 cacccaaaaa ctcacgggca taagagacaa aactgccact ggaagggcgg tgtagcacca
     5161 gctcacccaa tgcacgcaga ataaaaaacg aaaacaagcc acaaattaaa taaaccagtg
     5221 ccagtgctgg acccgccatt tgcagtcggg ctcctgcacc taaaaacaag ccggtgccaa
     5281 tcgcgccgcc aatggcgatc atctgtacct ggcgattgcc catcgctttg tgatacccct
     5341 cttcgtgggc attaagccag cggcgtttcg cggcgtgttg atctgaagtg tcggtgtcgt
     5401 gtttactcat tgctctccct gattgcttta atgaaaaagt catataagtt gccatgaaca
     5461 atgtttattc atcctggcga tgatagttat caccattatt ctttatgggt ataaaactat
     5521 cggcgcagca tcctacccgt tatcgatata cgatgcaaaa catcccctta caatcctgaa
     5581 ggggattcat acaactgacg aaaaaatgac aaatactttt gctggttaac ctgtgtactg
     5641 tcctacactt aatctttaaa agattgtgag gggcatatga ttaaggtgta tggcgtaccc
     5701 ggatggggct ctacaatcag tgagctgatg ctaacactgg ctgatattcc ttatcagttc
     5761 gttgatgtca gtggttttga ccacgaggga acttcacgcg agttattgaa aaccctgaac
     5821 ccgttgtgtc agattccgac actggcgctg gaaaatgacg agattatgac ggagacagcg
     5881 gcgattgcat tgatggttct cgatcgtcgc ccggaccttg cgccaccggt tgggcgcgcc
     5941 gaacgtcagc agtttcaacg gctattggtc tggctggttg ctaatgtcta tccaacattc
     6001 actttcgccg attaccctga gcgttgggct cctgacgcac ctgaacagtt aaagaagaat
     6061 gttattgaat atcgtaaatc gctttatatc tggctgaatt cacaattgac cgctgaacct
     6121 tatgcgtttg gtgagcaact aacgctggtg gactgttatc tttgcactat gcgcacatgg
     6181 gggtctgggc atgaatggtt tcaggataat gccacgaata tcagtgcgat tgctgatgct
     6241 gtatgtcagt taccgaaatt acaagaagta ttaaaaagga atgaaattat ttgagtggga
     6301 taccagagga ttatgttatt tatta
//
LOCUS       DAZPAC010000225         6305 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZPAC010000225 DAZPAC010000000
VERSION     DAZPAC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.226"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..277)
                     /locus_tag="ACHUPM_003720"
     CDS             complement(<1..277)
                     /locus_tag="ACHUPM_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871387.1"
                     /translation="MTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNAHILE
                     HGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVS"
     gene            complement(336..4193)
                     /gene="sigA"
                     /locus_tag="ACHUPM_003721"
     CDS             complement(336..4193)
                     /gene="sigA"
                     /locus_tag="ACHUPM_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR0871388.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4554..5558)
                     /locus_tag="ACHUPM_003722"
                     /pseudo
     CDS             complement(4554..5558)
                     /locus_tag="ACHUPM_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5543..5920
                     /locus_tag="ACHUPM_003723"
                     /pseudo
     CDS             5543..5920
                     /locus_tag="ACHUPM_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5959..>6305)
                     /locus_tag="ACHUPM_003724"
     CDS             complement(5959..>6305)
                     /locus_tag="ACHUPM_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0871389.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1572 a         1408 c         1411 g         1914 t
ORIGIN      
        1 gggagacaat gctgtattct tttttaaagc actcaccaga aacggtgatt gtaatgataa
       61 tggtcttctc gatcgagatg taaatacccg tttcgttata ctgtccgtat cggaaccatg
      121 agaactcagt tgcattccag ccatgctcca gaatatgtgc atttttttgc ctgatagttt
      181 ctttttttat tcgctcatgt ttaaggcata acaggcagat aaataatgtc agaaaaagaa
      241 agctaataat gagagcaaga gcgatatagt ctgtcacagg gtaatacata ttctctacgg
      301 tagaagaagg gccgcaaacg cggcccgggc tgttatcaga aagagtaacg gaagttggcg
      361 ttgatcgcgt tatccacgtt gtatttacca aatgccgatt tctcaaactc aagaccgaag
      421 cgaagattat cgcgaatttc ggcgttgaga ccaacattca tgagaatacg accgtctttt
      481 tcacctttga tacgtttctc accggacgca tcacgcagta cggtttcacc gttggcaaac
      541 aggtcaaact ggtagccaag gccggcgcgg gctgtgactt tccagtcctt accggagaag
      601 gatttaccca catcaacacc ggtacgccca atcagcggat taaagtcctt atccttcatg
      661 gtcacgctca ttccgcgatc ctgccagtcg aaccgtttac ctgatacggc cccataaacc
      721 agttctgcct gcggctcaat ccaggagtct tctgttacat gatagcggta gcctgcttca
      781 gcacccacat acaaggaatg agaactgtag tcttttgttc cgagtccagc aaaggtcgcc
      841 gagtactcat tatcatggtg aacatactta ctaatcaggt cgatataggc accagagtca
      901 aatatagcgg aagcatacag acctgccccg acggacttcg tcttgccact gaatgcatta
      961 ctgctggcat gactgtcggt atacgtcata gtcagaccag tgaaaaggtc aagtccatcc
     1021 agctcatgtt ttttatccac accaatctgc acatgggtgt agttgtcact gtatccacta
     1081 gaagctgaac ctgctccgct catgatgcgg gcccatgcac cggcctcgcc attggtgtca
     1141 cgcagatccc ccattcgttt attcaggttg ttgacttctg tcaggaaggc tttgtaatta
     1201 ccggacatat acgattttgc tgcaccggaa gatgctgtat tttcagccac cttatatccg
     1261 gtcagggtcc acgtggtatg ttcctgatcc tctttcgtgc tgatcacagg agatatgttg
     1321 ctgaaaccta tggcctgagt actggcttta aatatctctt tattgcttcc ttttggggct
     1381 gttatcaggt caacgtttaa tcctgagtta ttatttccca ctttctgtat atcgaccgac
     1441 agactgttat tgcttccggt taactgattt ttaacaatga gtttatccga ttgatttaca
     1501 gactggctca gatcagcccg cataatcaca gcagtattag tggcatccag tgtgtttact
     1561 gtcacagtat ggaaacccga attctctatg ttactaaaca acaaaagact gtcgcgggtt
     1621 tttaatgatt gcagggctga ctgtccgttt actctccata aggtgttata catgctggct
     1681 gaggcagcag gagcatcaat acctccggta taagaagtat caaagccacc cagcaaacct
     1741 gtagccagcg gtgtgtgtgt ggttttgtcc aggttatctt ctttatactg gtcaacacca
     1801 aacagaatag atgctttttt gtctgatttt atattaccat tgacccaggc ataattttga
     1861 gcctttaacg tggcaccatc ttctgtcata ttatatccat ctgacatata gattgccggg
     1921 ctgtatttat tattttctgt aggggtgcct gttaatgtca gggttccggc cagttcaata
     1981 gctttatcta catacatttt tccctgggcg gtaacagtag ctcctttgtt gataatcagg
     2041 tctgtattaa ttaaggataa tgcaccagtc atagtaacag ttgatttatc accaacagtt
     2101 attttgctgt tttgagcact cagactgccg ttgaaactac tttcgcctgc tgaatccccg
     2161 gagcgaagct gttgtctgaa tccaaaacct tcgccagtaa tgtttcttcc tgagaacatg
     2221 tcaatgtatg cagttttatt tcccagaaca atttgagagt ttttagcctc tatgtgtcct
     2281 tttacattag catcccggcc tatactcagg gttgcgtttt ctaaatttaa tttccggaaa
     2341 ttaaatttac gactttccca gtcgggctgg gaaaaagagg ctatctgatt gttggactta
     2401 tattccgtat tgtaacgttt atttacagtg ctttcctggt cctttatggc agcagaatag
     2461 tctttttgac ataaaaaagg aattggacag ttgttattgc cttctttaaa tatggcatgt
     2521 tctgtcgcat gtccttgtaa tgttaactgt gcattccgga cagagatatc gtttttgata
     2581 tcgactgagc catcaaagat cagattggca ttattgttat gttgctgggt agtattgata
     2641 atatgattta tgcttatatt acctgataca ttcccatggt acatatatgc atcagtatta
     2701 gtaagtgata atgttgattg cttcacgttt gagttcgtta cggttgttcc cgcatctgtt
     2761 gctgcgattt tctgaaatga ctggtcatag ccgtttagat ccagtgttcc gccatttctg
     2821 gtaaaataga tttcgccatt accgctttcg cttaaagcgt ctttggcatt tatttttacc
     2881 gtacctttac ctccggccat gtaaatttta ttgaaggctt tttcagcact aaggatgaca
     2941 gtcccattac ctattttaag gttatttccc tgtgctattt ttacatccag agtaccacta
     3001 cctattttat gcagattatc tccggcaacg cccttaacat tccattcaac ggtggtatta
     3061 ttatcaatat ctatcccggc acctttataa gacttatctt ttccgtacac tttatatgtc
     3121 tggttcttat cgaatataag cccgccaatg ccaagatcgg tatcctgctt gagcgttaat
     3181 tcccctcctc ctgagacaga caggtcctta tcttttataa tcgatgataa ctcttgtttt
     3241 ttaccatcca gctgcatagt gtttgtgaaa ttctttttta aattgtttac cagaatatta
     3301 ttaaataacg agtagtttgt ccaggctata ttggtactac tgagaaaacc aactgtactg
     3361 gtaacaccat ggaaaaccca tttttgttgt tctttatcat atagataaga accagaacca
     3421 ctatcgccaa aaattggtga gtttttaaaa ggggttctgt tggtataaat gtaactcttg
     3481 tttttccagt ccagattaaa aagacttgcg cttaacaagt cgggattata gttttgtcca
     3541 aacagatatt gtttgccgtt taatatcgtt gaggttgttc cggcacctgc tctgaagcca
     3601 attatttgtt ccttaccgtt gtagtttacg ccatatcttt tcatcgcatc ttcgggagaa
     3661 agtgagaagt cggcactctc cgtaacccct gttgtttcca cgacgaattt attcagacga
     3721 tgaaccgaaa agtcgccaga tgatactctg ttacttgctt tatatgctgt ctgatcccat
     3781 gactgtgttg ttattgcatg atgctgtgta ccgttatgag tcgcagtaac actgtacgca
     3841 cctccaattg atgttgttgc acctttgttg gaaacagcag aaaaatcagg tacaggtagt
     3901 tctggaaaat gaaatatctt tccatctttc atcattaatt gaacatttgt tgcacccggt
     3961 ctgaactctc ctctgttttg agcaaggtca agataatcgc gggtccatat attatttata
     4021 tccagcattg ctgcaaatgc tgtttcgcca taacttccat atattgagga taaaataata
     4081 ccgaggataa ctttctttct tgatgtaccg acactaactt tccgggtcag ttcagaaaca
     4141 gcaactaatc cacctgtaat atgactatat ttcagtgaat aaattttatt catacgtgaa
     4201 ctccgatatt tctgagttgt ggttatggga taacatttaa ttacaacggg gaactatcag
     4261 tcatggtacg taactgataa gggtataatg ataatcactg tcagttatat ttgtatgaca
     4321 gatgtaacaa aatgaaaaaa aagataagca caagtgattg attggtcaga tattgatcac
     4381 ttggtgtttg tattttgaac tttgcgatca aaatttgatc tttgttgttt ccggtcaatc
     4441 ctttacaggt tttctgtatt atccggggga gttaagtctg tcgatgtccg acagactttt
     4501 gatgatggaa acaacatgca gcgataatgt gcatgctgtt ttgcaggtgg aactcagaag
     4561 gtgaactgga actgtgcgac cgcaccgtaa gtgttgtccg tgccgatcag gcctgcaaca
     4621 tcaaggtgaa ccacatcaaa cggtgagatc cccaccccgg ccgtgaatgc actgccgtcg
     4681 ttaccggcca gattctgacg gtaaccggcg cgcagctgtg cccatttcca ggcattaaat
     4741 tctgcgccaa tggcggcaaa ctgacggttg ctgtcggagg tgaaaccgct ggccggggtc
     4801 agatccacat caaatgcggt ggtgaacatc gcgttgtgcc aggagacacc ggccgtcacc
     4861 tgcgggcgga tcctgaaggt ttccgtgata ccgttcactt cttttgtctc aatactacgg
     4921 gggatcaggt tctgtgcgac cagtcccagc gtccagttgt catccaggtc cgtactggca
     4981 ccgatgtcgg cgttgatgcc atttttcgtg ttgtggtagc ggtcgccttt gaaggcgctg
     5041 ctgtcataat ttctgaccag cacgttgtag ttgaacaggt cgacgcgctg gaatttgggg
     5101 gggaagccca gagaccattt ctgcccggcc gtttccagtt ccgtagccag agcaataccc
     5161 acatcagtga tcagtgccgc gcgggcaaag gcctgtgatg tcaggcggct tttgtccacc
     5221 tcctgcccgg tgctgtttgc cacctcttcc aggtaagtga ggtcagcatc agagacttta
     5281 ccgtcgacac tgacggtgcc gtgtgattta accatcagtg cggcagagag gcgatcaccg
     5341 ggaagggccg ccacagcaga aactcccagc tgtgcggcgg catgtgtgtt acggaagtcc
     5401 cggaggtgtt ctttcaggcg tgcggcagcc tcaggaacac cgtggttact ttcgatggcg
     5461 cggtcgaagg ccttccactc atcactgatc cggtcggcgt tgtcggtgat attatccgga
     5521 ttggagagct gcgcgccgac ggacggaagc aggatgctgg gctcatgcgc gacgcaaaat
     5581 ccacgatgta tatatcagta ctaaaagcgc gacggcggaa gaagctctga aactaatcgg
     5641 tgaactgtac gccatagagc acgatatacg cgggttaccg gtgtctgaac gcctggcggt
     5701 caggcaaatg cagagtagac cgctcctgac ttccctgtat aagctgatgc aggagaaaga
     5761 acacacgtta tcgaagaaat gtcgtctggg agatgcgttc cggtatatca ggaagcactg
     5821 ggctgcgttg tgcaacttct gtgatgatgg cctggctgag gtagataata atgcagcaga
     5881 aagagcgctt cgtgcagtct gtctcgggaa gaaaactttt tcgaggatca ctcattccgt
     5941 gtctgactgc ttcagggttt aattttgctt ctgctgactg aatgatgatt gggcagcaag
     6001 tgcaatatcc cacataaatc cagttaactc acgagccacc gccgtaatgg ctacttgtga
     6061 ttttttccct tttgctgtca gagttcggta acgagaacac aatcgttgtt gagctttcca
     6121 tgcaatatct ttggaatatt gtgttacaga gtccggtcgg taatatataa gccatgctcc
     6181 aacttttgca ggtgtacgat aagaccaagc ggcttcgtaa agcagacgtc tcagttcgct
     6241 gtttccaact tttgttattc ctctgggacg aatactgttt ccactggaat attcacctgg
     6301 cacca
//
LOCUS       DAZPAC010000226         6302 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZPAC010000226 DAZPAC010000000
VERSION     DAZPAC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.227"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            60..989
                     /gene="pfkB"
                     /locus_tag="ACHUPM_003725"
     CDS             60..989
                     /gene="pfkB"
                     /locus_tag="ACHUPM_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR0871390.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1090..1380
                     /gene="ghoS"
                     /locus_tag="ACHUPM_003726"
     CDS             1090..1380
                     /gene="ghoS"
                     /locus_tag="ACHUPM_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR0871391.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1486..2346
                     /locus_tag="ACHUPM_003727"
     CDS             1486..2346
                     /locus_tag="ACHUPM_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR0871392.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2387..2923)
                     /locus_tag="ACHUPM_003728"
     CDS             complement(2387..2923)
                     /locus_tag="ACHUPM_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR0871393.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3070..3738
                     /gene="hxpB"
                     /locus_tag="ACHUPM_003729"
     CDS             3070..3738
                     /gene="hxpB"
                     /locus_tag="ACHUPM_003729"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR0871394.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3901..4491
                     /locus_tag="ACHUPM_003730"
     CDS             3901..4491
                     /locus_tag="ACHUPM_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0871395.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4624..6015
                     /gene="tcyP"
                     /locus_tag="ACHUPM_003731"
     CDS             4624..6015
                     /gene="tcyP"
                     /locus_tag="ACHUPM_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFR0871396.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6019..>6302)
                     /gene="ydjO"
                     /locus_tag="ACHUPM_003732"
     CDS             complement(6019..>6302)
                     /gene="ydjO"
                     /locus_tag="ACHUPM_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFR0871397.1"
                     /translation="NINLLFNPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLSFSLQ
                     AHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         1545 a         1548 c         1581 g         1628 t
ORIGIN      
        1 aatttcttca ctttccgctg attcggtgcc agactgaaat cagcctatag gaggaaatga
       61 tggtacgtat ctatacgttg acacttgcgc cctctctcga tagcgcaaca attaccccgc
      121 aaatttatcc cgaaggaaaa ctgcgctgta ccgcaccggt gttcgaaccc gggggcggcg
      181 gcatcaacgt cgcccgcgcc attgcccatc ttggaggcag tgccacagcg atcttcccgg
      241 cgggtggcgc gaccggcgaa cacctggttt cactgttggc ggatgaaaat gtccccgtcg
      301 ctactgtaga agccaaagac tggacccggc agaatttaca tgtacatgtg gaagcaagcg
      361 gtgagcagta tcgttttgtt atgccaggcg cggcattaaa tgaagatgag tttcgccagc
      421 ttgaagagca agttctggaa attgaatccg gggccatcct ggtcataagc ggaagcctgc
      481 cgccaggtgt gaagctggaa aaattaaccc aactgatttc cgcttcgcaa aaacaaggga
      541 tccgctgcat cgtcgacagt tctggcgaag cgttaagtgc agcactggca attggtaaca
      601 tcgagttggt taagcctaac caaaaagaac tcagtgcgct ggtgaatcgc gaactcaccc
      661 agccggacga tgtccgcaaa gccgcgcagg aaatcgttaa tagcggcaag gccaaacggg
      721 ttgtcgtttc ccttggtcca caaggagcgc tgggtgttga tagtgaaaac tgcattcagg
      781 tggtgccacc accggtgaaa agccagagta ccgttggcgc tggtgacagc atggtcggcg
      841 cgatgacact gaaactggca gaaaatgcct ctcttgaaga gatggtccgt tttggcgtag
      901 ctgcggggag tgcagccaca ctcaatcagg gaacacgtct gtgctcccat gacgatacgc
      961 aaaaaattta cgcttacctt tcccgctaac aaaaacattc ccccagcatt gggggaatca
     1021 ccaccaacct gtcggcaacg cgtttctccg actttgctca aaagtcatgt gataacaaag
     1081 gggtgaacta tggccagtgg cgatcttgtc cgttatgtca taaccgtcat gttgcatgag
     1141 gatacattga ctgaaattaa cgagttgaat aattacctga ctcgcgacgg ttttttgctc
     1201 accatgacgg atgatgaggg aaatatccat gagctgggga ctaacacttt tggacttatc
     1261 agtacccaaa gtgaagaaga aattagagaa ctggtttcgg ggcttaccca aagtgcaacc
     1321 ggcaaagatc ctgaaatcac catcacgact tgggaggaat ggaatagcaa cagaaaataa
     1381 atggtttttg ggcaataatc agtctgtggt gtgcgttagc tcgtgttttt acaccgcatt
     1441 cttgcgctaa ccttatgatc tggcagacaa catgggagag acatcatgtg gcaggcaatc
     1501 agtcgtcttt tgagcgagca gttaggtgaa ggcgaaatcg aactgcgtaa tgaactgcct
     1561 ggcggagaag tccatgccgc atggcatttg cgctatgcag gacatgactt tttcgtcaaa
     1621 tgtgatgaaa gggaactgct tcccggtttt accgccgaag ccgaccaact ggagttactg
     1681 tcgcgtagta aaaccgtcac cgtgcctaag gtttgggcag taggcgctga ccgtgactac
     1741 agttttctgg tgatggatta tctcccacct cgtccgctgg atgcgcatag cgcatttatt
     1801 cttggtcagc aaattgcgcg tttacatcaa tggagtgacc aaccacaatt tggcctcgat
     1861 tttgataact cgctctccac aactccacag cccaacacct ggcaacgtcg ctggtcaacg
     1921 ttttttgctg aacaacggat tggctggcag ttggaactgg cagcagagaa agggatcgct
     1981 ttcggcaata tcgacgccat cgtcgagcat attcagcagc gtctggcctc gcatcaactg
     2041 cagccttctc tgttgcacgg cgatttatgg tccggcaact gtgcgctggg tccggatggc
     2101 ccgtacattt tcgaccctgc ctgctactgg ggtgaccgag agtgcgacct ggcgatgtta
     2161 ccgctgcata ctgaacagcc gccacaaatc tatgacggct atcagtcagt atccccgcta
     2221 cctgccgatt tccttgaacg tcaaccggtt taccaactct acacgctgtt aaatcgtgca
     2281 agattatttg gcggtcagca tttggttatt gctcagcagt cattggatag attattagca
     2341 gcatgatatg ggttgaggat aatggccgct ccgtgcggcc ttttgattaa ataaacccaa
     2401 gcagagaaaa gaacacataa cctgcagcaa tacaaataac cggcaggata tatagtgaga
     2461 aaaactgcag gaaaatagta tggtgcggaa caacaatacg ggactcaatt tgttcacgcg
     2521 ttagcccctc ttcccctttg gctttttcca gaatgagttg atcttcaaca ccttcccgca
     2581 agaagcgcgc ctggcgactc atccgggcac cggaatcctg caacgccagt ccgacaaaaa
     2641 tcaggatgaa aatcacccag aacataacgt tcacgccacc attaaaatta ggggtcgggg
     2701 agttatacca gaacagattc aaaaaaggcg tattcgcctg catcatatcg atcatgacat
     2761 gagtaaagtc gagcatgacc gcattaatgc cttcctggtt ttcctgccgg gtattcataa
     2821 atttcagcaa agaaatcaat gtggagatca atgcaggtat aaaaatcacc caacccaaaa
     2881 tccttttcaa aacagcaatg cgtccagctt gttgatacgt catgagttct ccttgattaa
     2941 gacgcgtcgt ttcacttagt ttacctgtag atatctgttt tcgcccattc tttaaaggcg
     3001 atatgatagg cgcttaatca taagcacggc ttaatacctt acacataatg ctctaaagga
     3061 gaggttgtaa tgtcaacccc gcgtcagatt cttgctgcaa tttttgatat ggatggatta
     3121 cttatcgact cagaaccttt acgggatcga gccgaactgg atgtgatggc aagcctgggg
     3181 gtggatatct cccgtcgtaa cgagctgccg gacaccttag gtttacgcat cgatatggtg
     3241 gtcgatcttt ggtacgcccg gcaaccgtgg aatgggccaa gccgtcagga agtagtagaa
     3301 cgggttattg cccgtgccat ttcactggtt gaagagacac gtccattatt accaggcgtg
     3361 cgcgaagctg ttgcgttatg caaagaacaa ggtttattgg tgggactggc ctccgcgtca
     3421 ccactacata tgctggaaaa agtgttgacc atgtttgact tacgcgacag tttcgatgcc
     3481 ctcgcctcgg ctgaaaaact gccttacagc aagccgcatc cgcaagtata tctcgactgc
     3541 gcagcaaaac tgggcgttga cccactgacc tgcgtggcgc tggaagactc ggtaaatggc
     3601 atgatcgctt ctaaagcagc ccgcatgcgt tccatcgtcg ttcctgcgcc agaagcgcaa
     3661 aatgatccac gttttgtatt agcaaacgtc aaactctcat cgctgacaga actcaccgca
     3721 aaagaccttc tcggttaatg accaggggca gtgatcacgc tgcccctggt tctttatctg
     3781 aatttgcatt caactgacgg attaatcgtc aatttaagag aaagagttac accgtcacca
     3841 cttccgtgca ctgtataaaa atcctatact gtacgtatcg acagtttagt gagttttatc
     3901 atgacggcgg aaggtcacct tctcttttct attgcttgtg cggtatttgc caaaaatgcc
     3961 gagctgacgc ccgtgctggc acagggtgac tggtggcata ttgtcccttc cgcaatcctg
     4021 acgtgtttgt taccggacat cgatcaccca aagtcgtttc ttgggcagcg attaaaatgg
     4081 atatcaaaac cgatcgcccg cgcttttggg catcgtggtt ttacccacag tctgctggcg
     4141 gtatttgcgc tgctggcaac cttttacctt aaggttccgg aaggctggtt cattccggct
     4201 gatgcgctac aaggaatggt gctgggttat ttgagccaca tacttgccga tatgctgaca
     4261 cccgccggtg ttcccctgct ctggccatgc cgctggcgtt tccgcttgcc tatcctggtt
     4321 ccccaaaagg gcaaccaact ggaacgtttt atctgcatgg cattatttgt ctggtcggta
     4381 tggatgcccc attcattacc cgagaacagc gctgttcgtt ggtcatcgca aatgatcaat
     4441 accttgcaga tccagtttca tcggcttatt aagcatcagg ttgaatacta aaaaggcaaa
     4501 aatcaccttt ctggaataag caattccatt tgaatataag agccagctca cagttctgtt
     4561 aatcttgcgc caacactatg actgctacgc agtgatagaa ataataagat caggagaacg
     4621 gggatgaact ttccattaat tgcgaacatc gtggtgttcg ttgtactgct gtttgcgctg
     4681 gctcagaccc gccataaaca gtggagtctg gcgaaaaaag tgctggtggg tctggtgatg
     4741 ggtgtggttt ttggccttgc cctgcatacc atttatggtt ctgacagcca ggtacttaaa
     4801 gattctgtac agtggtttaa catcgttggt aacggctatg ttcaactgct gcaaatgatc
     4861 gttatgccgt tagtcttcgc ctctattctg agcgcggttg cccgtctgca taacgcatct
     4921 cagttaggca aaatcagttt tctgaccatc ggtacgcttt tgtttaccac gctgattgcg
     4981 gcgctggtcg gtgtgctggt caccaacctg tttggtttga cggctgaagg tctggttcag
     5041 ggtggtgcag aaactgcacg tctgaacgcc attgaaagta actatgttgg taaagtctct
     5101 gatctgagcg ttccgcagct ggtcttgtcc tttatcccga aaaacccgtt tgccgatctt
     5161 accggagcca atccgacgtc aattatcagc gtggtaattt ttgccgcatt cctcggcgta
     5221 gctgcattga aactgctgaa ggatgatgcg ccgaaaggtg aacgcgtctt agccgctatc
     5281 gataccctac aaagctgggt gatgaaactg gttcgccttg tcatgcagtt gaccccttac
     5341 ggcgttctgg ctctaatgac caaagtggtt gcaggttcta acttgcaaga catcatcaaa
     5401 ctgggaagtt tcgttgtcgc gtcctacctc ggtctgctga ttatgtttgc agtgcatggc
     5461 attctgctgg gcattaatgg cgtgagtccg ctgaagtact tccgtaaggt atggcctgtg
     5521 ctgacgtttg cctttaccag ccgttccagt gctgcgtcta tcccactgaa tgtggaagca
     5581 caaacgcgtc gtctgggcgt tcctgaatcc atcgccagtt tcgccgcctc tttcggtgca
     5641 accattggtc agaacggctg cgccggtttg tatccggcaa tgctggcggt gatggttgcg
     5701 cctacggttg gcattaaccc gctggacccg atgtggattg cgacgctggt cggtattgtt
     5761 accgttagtt ccgcaggcgt tgccggtgtc ggtggtggtg caactttcgc cgcactgatt
     5821 gtgctgcctg cgatgggcct gccagtaacc ctggtggcgc tgttaatctc cgttgaaccg
     5881 cttatcgaca tgggccgtac ggcgctaaac gttagtggct cgatgacagc tggcacgctg
     5941 accagccagt ggctgaagca aaccgataaa gccattctgg atagcgaaga cgacgccgaa
     6001 ctggcacacc gttaattctt atgctggcaa cggtccgttt tgtatagggg ccgttgcttt
     6061 actttttaaa tgttccatcg cggcgatgca tactgactca tatttaaaca gatcgtcagc
     6121 gaaaaaaatg tccaacttat caatacattc ctggctcaat gaatgtgcct gtagtgagaa
     6181 gcttaaaacc tcatacccga gacgttttct aagtgaacta tctggaatac tcataatatc
     6241 ttcaagcaca ccacttccca ttcccggaag atttagtgga ttaaaaagaa gattgatatt
     6301 aa
//
LOCUS       DAZPAC010000227         6301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZPAC010000227 DAZPAC010000000
VERSION     DAZPAC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.228"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..222)
                     /locus_tag="ACHUPM_003733"
     CDS             complement(<1..222)
                     /locus_tag="ACHUPM_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFR0871398.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMA"
     gene            complement(236..979)
                     /gene="traF"
                     /locus_tag="ACHUPM_003734"
     CDS             complement(236..979)
                     /gene="traF"
                     /locus_tag="ACHUPM_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFR0871399.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(972..1229)
                     /locus_tag="ACHUPM_003735"
     CDS             complement(972..1229)
                     /locus_tag="ACHUPM_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFR0871400.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGIL
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(1256..3160)
                     /gene="traN"
                     /locus_tag="ACHUPM_003736"
     CDS             complement(1256..3160)
                     /gene="traN"
                     /locus_tag="ACHUPM_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFR0871401.1"
                     /translation="MKEKINTFFRSLNFNYSVKPCNLCAIVFFLSFSVFADSNSDYRA
                     GSDFAHQIKGQGSSSIQGFKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWA
                     TGETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEF
                     TNYTCERDTMVEEYCTRTASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPV
                     TGTVESASLSVYAAFFFLNSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLT
                     GSGCTANGNCLPHGNGDRKVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     ADGVLTGTECSEPGGTKTGVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLASRQCAFYSDEGTCLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLA
                     KCNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKHPDCRGITVDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQ
                     IADQLKQAGQ"
     gene            complement(3396..3776)
                     /locus_tag="ACHUPM_003737"
     CDS             complement(3396..3776)
                     /locus_tag="ACHUPM_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871402.1"
                     /translation="MTLPAGYYQIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLAWGWSVKGAFNSKFQLCFRLQPDTPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            complement(3773..4411)
                     /gene="trbC"
                     /locus_tag="ACHUPM_003738"
     CDS             complement(3773..4411)
                     /gene="trbC"
                     /locus_tag="ACHUPM_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFR0871403.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVARALLNNPGDKQK"
     gene            complement(4420..4725)
                     /locus_tag="ACHUPM_003739"
     CDS             complement(4420..4725)
                     /locus_tag="ACHUPM_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871404.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(4755..5747)
                     /gene="traU"
                     /locus_tag="ACHUPM_003740"
     CDS             complement(4755..5747)
                     /gene="traU"
                     /locus_tag="ACHUPM_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFR0871405.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(5744..>6301)
                     /gene="traW"
                     /locus_tag="ACHUPM_003741"
     CDS             complement(5744..>6301)
                     /gene="traW"
                     /locus_tag="ACHUPM_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFR0871406.1"
                     /translation="DLWPVKEPDMLTVIMQRLTALEQSGEMGRKMDAFKERVIRNSLR
                     PPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEGRVFARQGEVMNPLQYVPFNQTLYFI
                     NGDDPAQVAWMKRQTPPTLESKIILVQGSIPEMQKSLDSRVYFDQNGVLCQRLGIDQV
                     PARVSAVPGDRFLKVEFIPAEEGRK"
BASE COUNT         1535 a         1758 c         1458 g         1550 t
ORIGIN      
        1 tgccattgaa acaaccggta cggcacactg attcataacc gcaccacaaa gaaacaagaa
       61 attatctggc agcatacata cacttatgac atttactaca aagaagacaa acgagctcag
      121 cagataaact cctttattat ttggtcctgt aaagaaataa ccaacaaccc ccacgataaa
      181 caggctggca cctgtaaaaa aacacaaatc ccctgtatgc atatataacc tttggttaaa
      241 aattcggttt aaaatcttca gaaacgttca ggaactgttt tgccaggtcg tcctgtgaaa
      301 taaagccgta tgataacggg cgaacactgc cctgtttcgg gtcaaccagc atcatggccg
      361 ggaaatattt cacaccgagg cgctgcgcct gcccctggtc agtccgggaa tccggcaaca
      421 gcggattaat cacgccatcc acggaaacgg gaataacact cagaccatac gtatcccgga
      481 agccattaat gacctgcgcc agttgcccgt cgatggggtc ctgcccccgg taaaaaaaca
      541 tgatgccgta gtgttcagcc agtttcgcaa tggcctgtcg ctgctgcgcc tggtctgccg
      601 ccagctggtt ccgtaccgtg ccgttgtaat ggctgtactg caggttatag tccagttcgg
      661 gatgtgccag catggccttt ttagcactca tggtgaaaag cccggcctgc tgagtccagt
      721 aattctgcag ccggaaatat ttaacaaaat tatccacgcc gggatacaga atggcttcgt
      781 acagcgcccg ctttgttgcc gtctgcagcg cggccagttt ctgcataata tccggctcct
      841 gacggggggc tgcaggtaca ggtttatttt ctttttcctt cggatttatt ttctcgttat
      901 accactgcca gcctgcatct ttccctgacg ccggaaaaat aaagcagcag agtaacagtg
      961 gcagtaatgc tttattcatc ttcattcctt tcatcatttt ttctttcatc atctttctgc
     1021 aggccagtca gggcagaaaa acaaaaatac agaataaaca ggagcacacc ggagtaaagg
     1081 ataacgaaag cagcagcaag tatcccgtct gccgtagttt cttttaccgt atcccacgac
     1141 cagataatta ccggagaaat gacaatcgct gtaatcgaaa gccggataag aaaatcaaca
     1201 agacgattag aagtgaatat aactttcatc atttcccccg gttattttcc ataccttact
     1261 gtcctgcctg tttcagctgg tcagcaatct gctctttcac tttctgcgtc agaacgccac
     1321 tgtccgggat tttctggtta ttcatcaggt cttcgtagaa gttagtgaaa tccagtctgt
     1381 cgaactgaat tttctgcagc tcatcaaccg taatcccccg acagtcagga tgcttcgcac
     1441 tgccaaaccc gatacgcaac tgcccgttac gcccctgctg ctggactatc tgcgccagct
     1501 tcgaatcaaa ctggcagtag ctgcgctttt tctgcagaca gacacccagc actttccggg
     1561 agcagaactc gcccacactc accgtcagtt tgttgtcctt tgcctttccc agcgcctttt
     1621 cttcactgtt gcacttcgcc agccccacat cctgacccca gccgccatcc ttacagcagt
     1681 tactgaagcc ggctgcggcc ttacggcaga atttcgcctc acccgtgaaa gccctgacat
     1741 ccacaccatt cagggcggca acatccttac ctgcggcggc aagcgcagcc agctgtgata
     1801 ctgcttcacc aaaatcactg ttttttccgc tctgtgcctt atcgcattca ccgtcaaggc
     1861 agaagacatc accaccacag accatcactt ttcctgatgt tctggactcg caggagtacg
     1921 tggcgtactc atgcagacag gttccctcat cggaataaaa cgcgcactga cgtgaggcca
     1981 gcgtacaggc cggattatcg acatatttct gacaggtgcc gttatctgcg gactgagtga
     2041 catatttatc ccggtatgcc cagcatgcct gtgtgatgtt ccagggttta ccttccatca
     2101 ctccggtttt cgtaccaccc ggctctgaac actcagtgcc tgtcagcacg ccatccgctt
     2161 tattaaacgg acaactctcc acccactcca cccggggaac ccattcttta tcacggacct
     2221 tcatcctgag tttcagcgta aaggtggaag caccactgac aagcgattca tagactttcc
     2281 tgtcaccatt cccgtgcggg aggcaattac cgttagcagt acagccacta ccggtcagaa
     2341 cctgcccctg tgtcacctgc aaacctgtcg ccccggacag ggggtaagta tccgactgcc
     2401 cgtttgccag gccgacatta aaggttgtat tcatgaaggt ataacgggag ttcaggaaga
     2461 aaaaagcggc gtatacagac aatgatgcac tttccactgt accggttacc ggagccgtga
     2521 cactgaacac cagtttcagc ccgttcatac tgaaccggaa ctgtgagtgc ggtatcgtca
     2581 cttcacgata ttcatccgtg tatttccagt ccccggtgat actggcagtc cgtgtgcagt
     2641 actcctccac catcgtgtcc cgctcacagg tatagtttgt gaattcactg cggtttatct
     2701 cctgcgcact gcactgctgt ccggtatttc ccacaatgct gtcagccctg ttcaccacat
     2761 ccctgccggt ctgaatgaac ggtgcatccg gtgacagaat gtctttcggc ttgttcatga
     2821 aggactctgt gatggttttt ccggtttcac cggtcgccca ttccgtggtg ccgtcatttt
     2881 tcaggccgcc atccccgccg gcagtcacgc caccgtaata ttttgtctcg tccggattcg
     2941 cgttatagcc ggggatactc tcctgtggct taaagccctg aatactgctg cttccctgcc
     3001 ctttgatctg gtgagcaaaa tcagaaccgg cccggtaatc actgttgctg tcagcaaaca
     3061 cagaaaagct cagaaaaaaa acaattgcac aaagattgca cggctttacg gaataattaa
     3121 aattcagaga tctgaaaaag gtatttatct tttctttcat aacctgaacc atactggaga
     3181 atgaagtgat gtcatctcaa cttcagaact gtgttgattg tggtaaagca ttaagtcgaa
     3241 cagccacaac gtgcgggaat tgcagctcaa cggacccttt tggaaaaaaa cggcgcagcg
     3301 atcgctttta ccagtggttt gtagggctgt ttattacggc tattattatt gctggcggat
     3361 tatggtacat ggggcttctt gacgtaccgg gattactact cagataacga cttcagtaac
     3421 gatatcagtg ttctgaaatc ggcacataac gagtgacgac aataccggtt ataccagtaa
     3481 tgaggggtgt cgggctgaag cctgaaacag agctgaaact tactgttaaa agcccccttc
     3541 accgaccatc cccatgccag acgtggtatt gctgattcag catcctgccg cagtcgctgc
     3601 tcaagaaggg cagccatttt taccggacag gcaaaggcaa tataaggagg cagctttgta
     3661 acagggaaac catgtccctg acaggaagca tatgtgcgga agccgtgaat gttcatcgcg
     3721 gccaccaggg cacggatctc cggatcaatc tgataatacc cggcaggtaa agtcattttt
     3781 gtttatctcc cgggttattc agcaacgccc gagccacctg ccggcagtcc cccgtggcgg
     3841 ccactttctc cagcgcctgg ccgacccgca ggttacctcg gataatgtcg tatccctgac
     3901 tgcagaacac caccagtgcc ggtacactgc gaatgccgta ctgtgaaaac agcgtcgggt
     3961 cgatctgaac accatcagtc gcgccgtctt tcaccaggga cagcacggct tcagcggtgg
     4021 tcttcaggtc attattcacc atgccccgca gtgtggcggg tataccgtag tgccgggttt
     4081 cacccagcat ccgtttcagc ccctcttcgg gaatggaaaa cgacacaaaa tacagggcac
     4141 cctgccgggg tttcccgtcc tgcgaagcct gctgtttacg aaccagctca tccagaaaat
     4201 ggttatctga acgctgaagg gggttttcca gcacctgttt ctccgcccag gctttcagct
     4261 gatggtcagg tttctctcgc agttgtctgc ttaaattttc ctgctgcttc aggaactggc
     4321 ggttttcagg tgtgttcaca ttttctgaag catgaacagc aagatgcatc cctgctgtca
     4381 gagcaaaaag tgcaaatatt ttccggttca tgatatttct cactttctgc tatttacagg
     4441 catgaactca tcagaaaaaa gataattgag aaaaacagcc ataaacgcac atgtaaatgt
     4501 caccttcaga tataatctga caagtgtcac ttgttctgat atatctgcct tcttccattc
     4561 atgaataatt tcagcgagaa aactgtcagg tgcaaaataa aataatataa cgggcaccat
     4621 tacaagacca aaaggaaata attttaatgt tctgcttatc agcctgagca gcacagcact
     4681 tattttcctg aggtgctgaa tatatttact gtccattgtt gccattttca tttcctctta
     4741 ttcattaaaa tgatttacag gaatacacag ttacgtttac gccacatcag gtagccgaag
     4801 tttttctttg tgttgggcgg attttttccc gtctcccagc gcatcacact gcgcccgaac
     4861 ggatggcact gcccgctgtc cggatacata ttcaccatct gataacgcca gcgttctttg
     4921 ggcaggattg gggacggata ttcattacag acggcgttat ttttcccgat ggtttccata
     4981 atcatgccct gacggtgtag tttgaacgcc atgcgttcac tgaccagcag ggaggactgc
     5041 aacggactgg actcattact cacccagcca ttgaacgggt acatacttcc ctgcgaaccg
     5101 gcacaccaga acagaacatc gagaggcata ttaaaggcgc tggcaatggc atctgctgca
     5161 caggccccct gtgctatcgg attggcaaag atgacagctt ccggattgag aatggtggtc
     5221 aggctgctgt ccgtccaggt ggggtcaatt tcagaaagat aagcgatatc caggtcacca
     5281 ccttccagac agcccagcga tgtgatgatg ttcagccagt acgtcagcgg gtatttgtac
     5341 cagtgaacgt gatagaacgc cccgttcacc tgcttctcat cctttttcgc cgttccctgt
     5401 gccgttttac caaaagccgg caggctgaag cccaggttaa ccatgcatcc cggtgaccgg
     5461 gtgacgtccg tcagcgccat cggctcccag taaccaatgg ccagcccgat acgcctgaac
     5521 agcggcggcg gtgccggaca aatctgaatg ggcatcgacg ggttcgccgt gtcggggacc
     5581 ttaccctgac tgactttgac actgcccagc gagagcggga aaatacagct ccagcagata
     5641 tccgtgatcg ggtttacaaa acgcccctca caggcagaat ccgcagacgc tgcagggaca
     5701 tgaccggcga gaaggaataa catcagcagc cacaggcttc gcttcatttt ctgccctcct
     5761 ctgccggaat aaattccacc ttcaggaagc gatcgccggg aacggcgctc acccgtgccg
     5821 ggacctgatc aatgccgagg cgctggcaga gcacaccgtt ctggtcaaag taaacacggc
     5881 tgtccagaga cttctgcatc tccgggatac tgccctgcac gaggataatt ttgctctcca
     5941 gtgtgggcgg cgtctggcgt ttcatccagg ccacctgcgc cggatcatcg ccattgatga
     6001 aatacagcgt ctggttaaaa ggcacatact gcagcggatt catcacttca ccctgacggg
     6061 caaacacccg cccttcgtta tcccggatat ccgcagccag tctgacggac gggtcaaaca
     6121 accggctgcc gtatttctcc gtgcgtccga taccgggcac ggcagggggc cgcagactgt
     6181 tgcggatcac ccgctcctta aaggcatcca ttttccggcc catctcaccg gactgctcca
     6241 gtgccgtcag gcgctgcatg atcaccgtca gcatgtccgg ctcctttacc ggccacagat
     6301 c
//
LOCUS       DAZPAC010000228         6256 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZPAC010000228 DAZPAC010000000
VERSION     DAZPAC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.229"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            122..952
                     /locus_tag="ACHUPM_003742"
     CDS             122..952
                     /locus_tag="ACHUPM_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFR0871407.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHUPM_003743"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHUPM_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR0871408.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="ACHUPM_003744"
     CDS             complement(2081..3541)
                     /locus_tag="ACHUPM_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR0871409.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHUPM_003745"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHUPM_003745"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR0871410.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..>6256
                     /gene="gntP"
                     /locus_tag="ACHUPM_003746"
     CDS             5146..>6256
                     /gene="gntP"
                     /locus_tag="ACHUPM_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR0871411.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISA"
BASE COUNT         1480 a         1619 c         1588 g         1569 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcac caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttaat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgctg
//
LOCUS       DAZPAC010000229         6202 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZPAC010000229 DAZPAC010000000
VERSION     DAZPAC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.230"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(25..576)
                     /locus_tag="ACHUPM_003747"
     CDS             complement(25..576)
                     /locus_tag="ACHUPM_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0871412.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQMLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            1163..1510
                     /locus_tag="ACHUPM_003748"
                     /pseudo
     CDS             1163..1510
                     /locus_tag="ACHUPM_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            1563..1958
                     /locus_tag="ACHUPM_003749"
     CDS             1563..1958
                     /locus_tag="ACHUPM_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR0871413.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1999..2742
                     /gene="cmoA"
                     /locus_tag="ACHUPM_003750"
     CDS             1999..2742
                     /gene="cmoA"
                     /locus_tag="ACHUPM_003750"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR0871414.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2739..3710
                     /gene="cmoB"
                     /locus_tag="ACHUPM_003751"
     CDS             2739..3710
                     /gene="cmoB"
                     /locus_tag="ACHUPM_003751"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR0871415.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3875..>6202)
                     /gene="torZ"
                     /locus_tag="ACHUPM_003752"
     CDS             complement(3875..>6202)
                     /gene="torZ"
                     /locus_tag="ACHUPM_003752"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR0871416.1"
                     /translation="DGKILTAERWGAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQ
                     VHTTTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPS
                     AIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVY
                     EQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPI
                     RSETIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYL
                     RGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHWML
                     VTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRA
                     IPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEM
                     IVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDF
                     DVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKL
                     IEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAP
                     DEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSIAN
                     GDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSS
                     QLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
BASE COUNT         1456 a         1587 c         1441 g         1718 t
ORIGIN      
        1 acatccatgt aactattcag taacttattt agcaagtctg taataagttg ctctagagat
       61 tcctagctct tcctgaatct gcaacctcgt tttaccttgt gaaataagca tctgtacagc
      121 ctctgaagaa gcttttacag gtcttcccat atgtacccct tcagctttag cacgttcaat
      181 accagccttt ctacgctctg ctatcaagct tctttcaagc tgtgccaacg ttgacataat
      241 gcctaacatt gcttcaccca tagcgcttga taagtcaaat ccttctctaa ggctgataac
      301 tgtcacacct ttagcctgta gcgctttaac cgttgataac acatcaattg tgtcacgtcc
      361 aagacggtct acagctacca caactacggt gtcaccttct cgtgcataag ctaacaaact
      421 gctgaaaccg tttcttaaag aagctttgac agaaccactg acagcttcat cagcgaacca
      481 tttagcaatg ttgtaccctg cctcttcaat ttgcatttgc tggttagtga tggattggtc
      541 atctgtactc accctgcaat atccaagtat cgccataatg ttccccttag aagtctcaaa
      601 agttagtctc ataagcttag ctgtctcata atccaaaaac aaccttttga gactacttga
      661 gggtgcattc taccgttacg ttgaaagtgt ctcaaaagga cttccttatg agactggtta
      721 atagagcgct atgaagattc aggagggtaa ctataaccat cactttctaa ctttcacgta
      781 ggaaatcgtt aagtttcttg atagttacac catgtgacca actatctata tatcgttata
      841 tagccctgta tatagcacta tatgtagtgt ctggagaggg acgactaaca cgatacagtt
      901 taccctgaaa agagacttca cagattcttt atatatttca ataagttatg ctaaaaagag
      961 actataccga tactgcatag aatttgtgta ggattgcata gatacttgga aaaacaccgt
     1021 taaattgcat agtcattcac ctgatattgt cctgttttta caggtagttg attcggatgt
     1081 tctaggaatc atcctcatag ttgattcggt tagttgggct tgtaccaacc cctaatcaac
     1141 ctcatagtgt atttagacct tgatgatttt agacagccgc cctgttcatg cagcacgtcc
     1201 acacagtgaa gctattcgcg acgctcaacg aaaaaaacct aaagttccgg tacatgctgt
     1261 actgacggcg acaaatccgc tgattcgttt tatcggtagt gatgatatga cgcaaaaccg
     1321 ggaattattt caggtctggt tacagaaatt agcgcagtgg catcagacca ctacgcctta
     1381 tcttttttta catacgccag acattgccca ggccccggaa ctggtacata ccctgtggga
     1441 agacttacgt aaaacgcttc cagagatcgg agcagttccg gctattccac agcaatcttc
     1501 tcttttctga atttgccacc tatcatagac aggtgccatc ggccatttta aagggagttt
     1561 gtatggtaag cgcgctatat gccgttttaa gtgcgttgtt attaatgaag ttctcttttg
     1621 atgtcgttcg cctgcgaatg cagtaccgcg ttgcctatgg tgacggtggt tttagcgaac
     1681 tgcaaagcgc tattcgcatt catggtaacg cggtggaata tattcccatc gcgattgtgc
     1741 tgatgctgtt tatggaaatg aatggcgcag aaacctggat ggtgcatatt tgcggcatcg
     1801 ttttgcttgc tggtcgtctg atgcattatt acggttttca tcaccgtctg tttcgctggc
     1861 gacgttccgg catgagcgcc acctggtgtg cgctgttgct gatggtgctg gcgaatcttt
     1921 ggtatatgcc ctgggagttg gttttctccc tgcgttagcg cacaatacgc cactttcttt
     1981 ttcccggatt tttacgttat gtctcaccgc gacacgctat tttctgcccc tatcgccaga
     2041 ctgggcgact ggacctttga tgaacgggta gctgaagtct tcccggatat gatccagcgt
     2101 tccgttcccg gctattccaa tattatttcc atgattggta tgttagccga gcgcttcgtt
     2161 caacctggta cgcaggttta cgatctgggt tgttctctgg gcgcggcgac gctctcggtg
     2221 cgtcgcaaca ttcatcatga taattgcaaa attattgcca tcgacaactc cccggcgatg
     2281 attgaacgct gcagtcgtca tattgacgcc tataaagccc ctacgccagt agacgtcatt
     2341 gaaggtgata ttcgcgatat cgccattgaa aacgcatcga tggtggtgct gaattttacc
     2401 ctgcaattcc tggaaccttc cgagcgccag gcattactgg ataaaattta tcaaggactg
     2461 aacccgggcg gcgcgctggt gctttcggaa aaattcagtt tcgaagatgc caaagttggt
     2521 gaactgttgt tcaacatgca ccacgacttt aaacgtgcca acggttacag cgaactggag
     2581 atcagccaga agcgcagcat gctggaaaac gtgatgctga ccgattccgt ggaaacccat
     2641 aaagcacgcc tgcataaagc cggttttgag catagcgagc tgtggttcca gtgctttaac
     2701 tttggttcac tggtggcatt aaaagcagag gacgctgcat gatcgacttt ggtaactttt
     2761 attctctgat tgccaaaaat catctttcac actggctcga aacgctgccc gcgcagattg
     2821 ctaactggca gcgcgagcag cagcacgggc tgtttaagca gtggtccaac gcggtggaat
     2881 ttctgcctga aattaaaccg tatcgtctgg atttattgca tagcgtaacc gccgaaagcg
     2941 aagagccact gagcaccggg caaattaaac gcattgaaac gcttatgcgg aacctgatgc
     3001 catggcgcaa agggccgttc tcactgtatg gcgtcaacat cgataccgaa tggcgttccg
     3061 actggaaatg ggatcgtgtt ctgccccatc tttctgattt aaccggacgc accattctgg
     3121 atgtcggctg cggcagcggt tatcacatgt ggcgcatgat tggcgcaggc gcgcacctcg
     3181 cggtggggat cgaccctacg caactgtttc tgtgccagtt tgaagcggtg cgtaaactac
     3241 tgggtaacga tcagcgcgca catttgttac cgttaggtat tgaacagctt ccggcactga
     3301 aagcctttga taccgtcttt tcgatgggcg tgctttacca tcgccgttcg ccgctggagc
     3361 atctctggca gttgaaagat caactggtga atgagggtga actggtgctg gaaacgctgg
     3421 ttattgatgg cgatgagaac acggtgctgg taccaggcga tcgttacgcg caaatgcgta
     3481 atgtctattt catcccttcc gcgctggcgc tgaaaaactg gctgaagaag tgtggttttg
     3541 tagatatccg cattgtggat gtgtgcgtta ccactactga agagcagcga cgcactgaat
     3601 ggatggttac cgaatctctg tccgatttcc tcgacccaca tgatccgagt aagacagttg
     3661 aaggttatcc tgcgcctaaa cgcgcggtgc tgattgcgcg caagccgtaa aggtctggta
     3721 ttactgccgg atgcggcgtg aatgccttat ccggcctaca aacccttgct aattcaatct
     3781 attgcaggga ctatgtaggc ctgataagca tagcgcatca ggcagtttta cgtttgcata
     3841 acctcagcgc ccgtttccgg gcgctattca cttcttacgc ctgaacagca ggcttatcaa
     3901 atgccgttaa cttcggtgcg ttacccgtat atttttcgat atacaccagc gcagagttac
     3961 cggcacaggc atttgccagc tgcgagctgg ggatatccgc cgttaacacg ttcgcactgc
     4021 cgtttttaca caagccgttt tccagatctg gccatgcacc ttcatgcacg cataccacgc
     4081 cttttttgat cccgtcagtc accaccgcgc ctgtcaaaat ctgaccacgt ttgttccaga
     4141 cacgcaccag atcgccattc gcaatactaa atcgagcagc atcttcggtg tgaatagtga
     4201 tcggttcacg atcggcaacc gcatattttt tacgcagttc cgcatagtta agctgactgt
     4261 gtaaacggtg tgccggatgt gcggtcagaa gctgcaactg cttctcgtcg gcggtaccct
     4321 tccactcatc aggcgcaagc caggttgggt gtgccgggca atccttatag ccaaattttt
     4381 ccagcgtttt ggagtaaatc tcaattttgc cacttggcgt acccagcgca tttttcaccg
     4441 gatcggcgcg gaaatcacca taacgaacgt actgttcgtt cttctcgctg cggcgcattt
     4501 cgatcagttt attttgctgc cagaaggcat taaacattgg catagtgacg cgttgcgcac
     4561 gggccccttt ctgagcggca tcatagaaaa atttcagcca cgccatttca tctttacctt
     4621 cggtatagat ctcttttccg ccaggtttga gtaattccgc aagatcggca aacacatcaa
     4681 aatcgtttcg cgcttcaaat tgcggagcaa cagcctgctt catcggcaca atatgctggt
     4741 tgctgtaatc accggtcatc gtcaagtcat tgcgctcaaa cgatgtggtg atcggtaata
     4801 cgatatctgc gtgtttagca gctgcggtcc agtagcattc agaaacgacg atcatctccg
     4861 gtttctgcca tgctttaatc agacgattag tatcctggtg atgggtaaag ttaccgccac
     4921 ccgcccacca gatcattttg atattcggat acgtttgctc tttaccgtta tgctgatatt
     4981 tgccgccagg attttccagc gcatcgacga tacgggcaac aggaatcgcc ctcattccgc
     5041 catcatcagc ggcctcgctg gcctgtccgg caatcgccgc agacatttcc ggcagcacgc
     5101 cgccaacacg tgtcgggttg ccaccattgg agtaatgata agagaaacca aaaccgccgc
     5161 ccggtgtacc aatctgccct aacatcgccg ccagcgtcac cagcatccag tgtttctgtt
     5221 caccatattg ctggcgctga attccccagc ctgccatcag cattgtgcga ttcgccgcca
     5281 tcagttcagc gagtttgact atctgcgctt ccggtacgcc agtaatttcc gccgcccagg
     5341 ccgcgctctt cggcgtatta tcgctcttac ctctcagata ctcttcaaac tgcggatacc
     5401 cggtagtgta tttttcaagg aacactttat cgtgtttgcc ttgtgtcatc agggtatgcg
     5461 caatccctaa catcagtgcc acgtcggtgc ccatattcgg cgcgatccag gtggcattat
     5521 cgccaaagaa gtcgatggtt tcggagcgga taggatcaat ggcaatcact ggtttgccag
     5581 attttttcag ctgatggaag tattccagcc cttgctcatc ggtactgctc caggcaattt
     5641 ttaaggtatt cagcgggttc atcccccaca gcaccacaac ctggctgttt tccagaatca
     5701 gcggccagga ggtctgctgt tcatacacct ctacagaacc gaccacatgc ggcatgatca
     5761 cctgtgccgc cccggttgaa taatcgccgc tatgcccgga ataaccgccc gccaggttca
     5821 tataacgttg cagtaaggtt tgcgctttat gcaacacgcc agaagagcgc cagccgtatg
     5881 atccggcaaa aatagccgat ggtccgttag ctttgcgaat gcgatcgtgt tgctcatgaa
     5941 tcagttttaa tgcttgttcc cagctcacct gtacataggt atcttcgcca cgacctttcg
     6001 tcggttgcag tggattatcg agatagcttt ttctcaccat cggatgctga atacgcgtcg
     6061 tggtgtgtac ctgatccgcc gccgtagact gtaaggaatt cggtatggtt ttcgccagcg
     6121 cgcctgttga agaaacaatc ttgccgtcct tcacttctac gttcatcgct ccccaacgtt
     6181 ccgcggtgag gattttaccg tc
//
LOCUS       DAZPAC010000230         6162 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZPAC010000230 DAZPAC010000000
VERSION     DAZPAC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.231"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            71..847
                     /gene="exuR"
                     /locus_tag="ACHUPM_003753"
     CDS             71..847
                     /gene="exuR"
                     /locus_tag="ACHUPM_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR0871417.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1087..1170
                     /gene="yqiM"
                     /locus_tag="ACHUPM_003754"
     CDS             1087..1170
                     /gene="yqiM"
                     /locus_tag="ACHUPM_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR0871418.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1192..1854
                     /gene="yqjA"
                     /locus_tag="ACHUPM_003755"
     CDS             1192..1854
                     /gene="yqjA"
                     /locus_tag="ACHUPM_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR0871419.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1858..2241
                     /gene="mzrA"
                     /locus_tag="ACHUPM_003756"
     CDS             1858..2241
                     /gene="mzrA"
                     /locus_tag="ACHUPM_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR0871420.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2388..2756
                     /gene="yqjC"
                     /locus_tag="ACHUPM_003757"
     CDS             2388..2756
                     /gene="yqjC"
                     /locus_tag="ACHUPM_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFR0871421.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2794..3099
                     /locus_tag="ACHUPM_003758"
     CDS             2794..3099
                     /locus_tag="ACHUPM_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR0871422.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3102..3506
                     /locus_tag="ACHUPM_003759"
     CDS             3102..3506
                     /locus_tag="ACHUPM_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR0871423.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3496..3795
                     /locus_tag="ACHUPM_003760"
     CDS             3496..3795
                     /locus_tag="ACHUPM_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR0871424.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3981..4373
                     /locus_tag="ACHUPM_003761"
     CDS             3981..4373
                     /locus_tag="ACHUPM_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0871425.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4443..5428
                     /gene="yqjG"
                     /locus_tag="ACHUPM_003762"
                     /pseudo
     CDS             4443..5428
                     /gene="yqjG"
                     /locus_tag="ACHUPM_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
     gene            5721..6086
                     /locus_tag="ACHUPM_003763"
     CDS             5721..6086
                     /locus_tag="ACHUPM_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0871426.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1545 a         1516 c         1549 g         1552 t
ORIGIN      
        1 atgaccaatg gcatacccat gaataaagtg gtatgataac ttcgtccgca ttccactccg
       61 ggagacgcat atggaaatca ctgaaccacg ccgtttgtat caacaacttg ccgctgacct
      121 gaaagagcgc attgaacagg gcgtctatct ggtgggtgat aaactgcctg ccgaacgctt
      181 tattgccgat gaaaagaacg tcagccgaac ggtagttcgt gaagccatca tcatgctgga
      241 agttgaaggc tatgtggagg tgcgtaaagg ttcaggtatt catgtggttt ccaaccagcc
      301 gcgccatcag caggcggctg acaataatat ggaattcgcc aattacggtc cgtttgagtt
      361 gcttcaggct cgccagctca tcgaaagtaa tattgccgag ttcgcggcaa ctcaggtaac
      421 gaaacaagac atcatgaaac tgatggccat tcaggaacag gcgcgcggcg aacaatgctt
      481 ccgtgattcc gagtgggatt tacagttcca cattcaggta gccctggcga cgcagaactc
      541 cgccctggcg gccatcgttg aaaaaatgtg gacccagcgt agccataacc cgtactggaa
      601 aaaactacac gaacacattg attcccgtac cgttgataac tggtgtgatg accacgatca
      661 aattctcaag gcgttaattc gcaaagatcc ccatgctgct aagctggcaa tgtggcagca
      721 tctggaaaac accaaaatca tgttatttaa cgaaaccagc gacgatttcg agttcaatgc
      781 cgaccgctat ctattcgccg aaaacccggt agtgcatctc gatacggcca ccagtggcag
      841 taaatgaaac tcctgtccga caggcgctgc gacgcgcctg ttcgcttcgt cggtaagcga
      901 aaagtataaa gtgtcagcct gtgtaaatcc tctcgccacc ctcccctgca ttcagcaaaa
      961 tcagactcca cggacatgta attttgataa cgaacaacgt tgacctttgt tacaattaga
     1021 ttcaatttga atttatgttt ttgaatgctt tcttatctca cgatttaaca gggaatagtt
     1081 caggctgtgt tgatgtatca aacccgcaga acataccaaa acagcaataa cattgcggta
     1141 gtgcatcttt taaaaccagc gtggcgttaa ccgattcacc aggaataatg aatggaactt
     1201 ttgacccaat tgctgcaagc cctgtgggcg caggattttg aaaccctggc caatccatcg
     1261 atgattggca tgttgtattt tgtcttgttt gtaattttgt tccttgaaaa cggcttgctt
     1321 ccagcggcct ttttaccggg cgacagttta ctggtattgg tcggcgtgtt gattgcgaaa
     1381 ggcgcgatgg gttacccgca aacgattctg ctgctgaccg ttgccgccag cctcggctgc
     1441 tgggtcagct atattcaggg gcgatggctg ggcaataccc gcaccgtaca aaactggcta
     1501 tctcatttac ccgcgcatta tcatcaacgc gcacaccatc tttttcataa acacggttta
     1561 tcggcgctgt taattggtcg ctttattgcg tttgtcagaa cactgctgcc gacgattgcc
     1621 gggttatcag ggctgaataa cgcgcgcttt cagtttttca actggatgag cggtctgctg
     1681 tgggtattga tcctgacaac tctgggttac atgctcggca aaacgccgat atttttaaag
     1741 tacgaggacc agctgatgtc atgcctgatg ctgctcccgg tggtgctgct ggtgtttggc
     1801 ctggcaggtt ctctggtcgt gttatggaaa aagaaatatg gaaatcgggg gtaagggatg
     1861 caaatacctc gcatgtcgct tcgccagcta gcctggtccg gcactgtttt acttctggtc
     1921 ggcacgctgt tgctggcctg gtccgcggtt cgccagcaag agtctacgct ggcgattcgt
     1981 gccgttcatc aaggcacaac gatgccagac ggtttttcga tctggcatca ccttgacgct
     2041 catggcattc ctttcaaaag tatcaccccc aaaaacgaca ctctgttaat tacatttgac
     2101 tccagcgacc agagcgccgc cgcaaaagcg gtcctcgaca gaacattgcc ccatggctac
     2161 atcattgcgc agcaggacaa taacagtcag gctatgcagt ggctgacccg gttacgggat
     2221 aactctcatc gcttcggata acttcctgtt atccgaaaca tatcactcac tttggtgatt
     2281 tcaccgtaac tgtctatgat taatgaggcg gtggttaaca ctaccgcttc gttgtctgga
     2341 caatcgttcc tttgtaatag gtccgaatca caatggaagg ttcaagaatg aaataccgca
     2401 tcgctttagc tgtttctctc tttgctctta gtgccggtag ttatgccact accctgtgtc
     2461 aggaaaagga gcaaaatatc cttaaggaga tcagctatgc cgaaaaacac caaaaccaga
     2521 atcgtattga cggtctgaat aaagccctga gtgaagtccg ggccaactgt tcagatagcc
     2581 agctgcgtgc cgatcatcac aagaaaatcg caaagcagaa agatgaggtg gcggaacgcc
     2641 agcaagattt agccgaggcg aagcaaaaag gcgatgccga taagattgcc aaacgcgaac
     2701 ggaaactggc agaagcgcag gaagagctga aaaagctgga agcgcgcgac tactaactca
     2761 caatagtcac tacttactca cctggagaaa actatgtcga aagaacacac tacggaacat
     2821 ctgcgtgctg agttgaaatc cctttccgat acgctggaag aggtgcttag ctcatctggc
     2881 gagaagtcga aagaagagtt gagtaagatt cgtagcaaag cggagcaggc actgaaacag
     2941 agccgttatc gcctgggtga aaccggtgat gccatcgcca aacaaacccg tgtcgcggcg
     3001 gcgcgtgctg atgagtatgt gcgtgaaaat ccgtggaccg gcgtgggaat tggcgctgca
     3061 atcggtgtag tgctcggcgt tctgctgtcg cgtcgttaat tatggcggac actcatcacg
     3121 cgcaagggcc cggtaaaagc gttctgggca tcgggcagcg aattgtttcc atcatggttg
     3181 aaatggtgga aacacgtctg cggctggcgg tggtggagct ggaagaggaa aaagctaatc
     3241 tctttcaact tttactgatg ctgggcctga cgatgctttt cgctgcattt ggtcttatga
     3301 gcctgatggt gctaattatt tgggcggttg atccgcaata tcgcctgaat gcgatgattg
     3361 ccaccaccgt ggtgttgctg ctactggcac tgattggcgg tatctggacg ttacgtaaat
     3421 cgcgtaaatc caccttgctg cgccatacac gccatgagct ggcaaacgat cggcaactgc
     3481 tcgaggagga gtcccgtgag cagtaaagtc gaacgtgaac gacgtaaggc gcaactgctt
     3541 agccagatcc agcaacaacg gctggatctt tccgccagtc gtcgtgaatg gctggaggca
     3601 acaggcgctt acgatcgtcg ctggaatatg ctgctaagtc tgcgctcctg ggcgctggtt
     3661 ggcagtagcg tgatggcgat ctggacgatt cgccatccta atatgctggt ccgctgggcc
     3721 agacgcggtt ttggcgtatg gagcgcctgg cgtctggtta aaacgaccct caagcagcaa
     3781 cagcttcgcg gttaacttcc ctctggccgg agcaattccg gccttatccc tcaaattttt
     3841 tgaagatttt tgacagtttt ccttgctaac aatcatcatt caccacgttt atgattctct
     3901 ccatcgacag caacgacgct aataccgcgc ccttgcacaa aaaaacaatc agcagcctga
     3961 gtggcgcagt ggagagtatg atgaaaaaat tagaagatgt tggtgtactg gtagcgcgca
     4021 ttttaatgcc gattctgttt attaccgcag gctggggaaa aatcaccgct tactctggta
     4081 cccaacaata tatggaagca atgggtgtcc cgggttttat gctgccgctg gtgattctgc
     4141 ttgagttcgg tggtggtctg gcaatcctgt tcggtttcct gactcgcacc acagccctgt
     4201 ttactgcggg ctttacgctg ctgacggcat ttttatttca cagcaacttt gctgaaggcg
     4261 tcaactcgct gatgttcatg aaaaacctga caatttctgg cggattcctg ctgctggcaa
     4321 ttaccggtcc gggcgcgtat agcatcgacc gcctgctgaa taaaaagtgg taagcacgcg
     4381 ctatacttaa cgataaaaaa cgaggaggaa gctcctcgtt tttgctattg gaggagagaa
     4441 aaatgggtca actgattgac ggcgtctggc atgacacctg gtacgatacc aaatctaccg
     4501 gcggtaaatt tcaacgttca gcttccgcat ttcgtaactg gctcactgcc gatggcgcac
     4561 ctggccccac tggcacaggc ggttttatcg cagagaaaga tcgttatcat ctctatattt
     4621 cactcgcctg cccgtgggcg caccgcacgc tgatcatgcg caaactcaaa ggactggaac
     4681 cgtttatttc cgtttccgta gtgaacccgc tgatgctgga aaacggctgg acctttgatg
     4741 acagttttcc gggagcaacc ggagatacgc tctatcaaca tgaatttctg tatcagcttt
     4801 atctccacgc cgatccacac tacagcggac gagttactgt tcccgtactg tgggacaaaa
     4861 agaaccacac catcgtcagc aacgaatcaa cagaaatcat ccgcatgttc aataccgcgt
     4921 ttgatgcgct gggcgcgaaa gcgggtgatt actacccacc agccctgcaa acgaaaattg
     4981 acgaacttaa cggctggatt tatgacaccg ttaacaacgg cgtgtataaa gctggttttg
     5041 ccaccagcca gcaagcctac gacgaggcgg tggcgaaagt gtttgaatcg ctggcgcgac
     5101 tggagcagat tttaggccag catcgttacc tgaccggcaa ccagctaacc gaagccgata
     5161 ttcgcctgtg gactacgctg gtgcgttttg atccagtgta tgtgacccac ttcaagtgtg
     5221 ataagcaccg catcagcgat tacctgaatc tgtatggctt cctgcgcgat atctaccaga
     5281 tgccggggat cgccgaaact gtcaatttcg atcacatccg taatcattac ttccgcagcc
     5341 ataagaccat caaccctacg gggattattt caattggtcc gtggcaggat ctcgatgaac
     5401 cgcaggacga gatgttcgct tcggttaaaa aaaggcacct tacgaagtgc ctctgataat
     5461 tagctaagtg catgttgttc atatcggatg cggcgtgaac gccttatcaa acctacaaaa
     5521 catttatatt caatatgttg caatgaccgc gcaggccaga taagcgtagc gcatctggcg
     5581 attttgcgct tgtcatcagt cttagcgcgc cttcacagca ctttattaat tccttgaacg
     5641 aatatttact gccatttcat tcacgtctat tcttaatttg ctgctttaaa aacaagtgat
     5701 tgagcaacat tgaggcaaaa atggactggt atctgaaagt aataaaaaat tatgtcggtt
     5761 tccggggtcg tgcgcggcgc aaagagtact ggatgtttat tctggtcaac atcatcttta
     5821 cgttcgtgct ggggctgctg gataaaatgt taggctggca acgcgctggt ggcgaaggca
     5881 tcctgacgac aatctacggt attctggtgt ttttaccgtg gtgggcagtt cagttccgcc
     5941 gcctgcacga caccgaccgc tcggcgtggt gggcactgct attcttaatc ccgtttatcg
     6001 gctggcttat tatcatcgtc tttaactgcc aggcgggtac gccaggcgaa aaccgctttg
     6061 gacctgatcc aaaactggaa ccttaaaaca aatctgctcg ccggatgtct gactcatccg
     6121 gcttttatca tcatgttgat gaatgaataa tttactaatg tt
//
LOCUS       DAZPAC010000231         6106 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZPAC010000231 DAZPAC010000000
VERSION     DAZPAC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.232"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHUPM_003764"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHUPM_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR0871427.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHUPM_003765"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHUPM_003765"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR0871428.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="ACHUPM_003766"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="ACHUPM_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR0871429.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3697..5964
                     /gene="adiA"
                     /locus_tag="ACHUPM_003767"
     CDS             3697..5964
                     /gene="adiA"
                     /locus_tag="ACHUPM_003767"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR0871430.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         1618 a         1619 c         1451 g         1418 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcagatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaataat ttaaaataat cacataacgc attgtttata aaaataaata
     3601 tttcaaaaat gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc
     3661 cgcaacgaag attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc
     3721 gagtttctcc atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta
     3781 agccagcaaa atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc
     3841 tcttcaaacg aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa
     3901 catcaaaacg tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc
     3961 ttcctgttgg gcgatcggga aaaagccctc gccgcaatgg atcgcgacct gctggagctt
     4021 gtcgatgaat tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgctgtt
     4081 gccgcgatga cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa
     4141 tatagcgaca tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt
     4201 accaaaacac ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc
     4261 gatatgggca tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc
     4321 gaaagcgaaa aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc
     4381 ggtacttccg gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg
     4441 gtcgttgacc gtaactgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa
     4501 ccggtctaca tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag
     4561 gaaatgcaac ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa
     4621 gccgggcaaa aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat
     4681 aacgctaaag aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa
     4741 gcctggtacg gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc
     4801 gaacctggcg atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg
     4861 aatgcgcttt cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc
     4921 tcccgcttca accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc
     4981 gcatccaacg acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacgcag
     5041 gaagtgatcg acgaagcggt tgatttccgt caggcgatgg cgcggctgta taaagagttc
     5101 accgctgacg gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa
     5161 accggcaaaa cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac
     5221 tgctgggtaa tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg
     5281 agtatgctcg acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa
     5341 ctggaagaaa ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt
     5401 gtgcctaccc gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt
     5461 gggaaatggg gaactctggt taacaccctt tgctccttca aacgccacta tgacgccaac
     5521 acaccgctgg cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac
     5581 atggggattc acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc
     5641 gcacggttga acgaagccta ttccggcctg tcggtggcgg aaatcacccc gcgtgaagcg
     5701 tacaacgcga ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc
     5761 atcgcggcaa actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa
     5821 aacttcggcg ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac
     5881 caccatttcc ctggatttga acacgaaact gaagggactg aaattattga cggtatttac
     5941 cacgttatgt gtgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac
     6001 attacgccgc ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca
     6061 aacaaaaatg tcatactttt tgcgcggccc caccccgcgc tttttt
//
LOCUS       DAZPAC010000232         6028 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZPAC010000232 DAZPAC010000000
VERSION     DAZPAC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.233"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="ACHUPM_003768"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="ACHUPM_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR0871431.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="ACHUPM_003769"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="ACHUPM_003769"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR0871432.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="ACHUPM_003770"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="ACHUPM_003770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR0871433.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="ACHUPM_003771"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="ACHUPM_003771"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR0871434.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="ACHUPM_003772"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="ACHUPM_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR0871435.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1430 a         1519 c         1637 g         1442 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaat atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatgga gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctg
//
LOCUS       DAZPAC010000233         6026 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZPAC010000233 DAZPAC010000000
VERSION     DAZPAC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.234"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1491
                     /locus_tag="ACHUPM_003773"
     CDS             <1..1491
                     /locus_tag="ACHUPM_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR0871436.1"
                     /translation="AEQEALIHALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVS
                     RRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAP
                     CCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERG
                     IAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEAL
                     HGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDR
                     KGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALT
                     EEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELA
                     KAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGALL
                     YSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
     gene            1529..1990
                     /locus_tag="ACHUPM_003774"
                     /pseudo
     CDS             1529..1990
                     /locus_tag="ACHUPM_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(2516..3691)
                     /gene="senB"
                     /locus_tag="ACHUPM_003775"
     CDS             complement(2516..3691)
                     /gene="senB"
                     /locus_tag="ACHUPM_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFR0871437.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(3760..6021)
                     /locus_tag="ACHUPM_003776"
     CDS             complement(3760..6021)
                     /locus_tag="ACHUPM_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR0871438.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYHSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
BASE COUNT         1485 a         1481 c         1523 g         1537 t
ORIGIN      
        1 gccgaacagg aagcgctgat ccacgccctg caggaaaagc ttagcaaccg ggagcgcgaa
       61 atagaccatc tgcaggcgca gctggataaa ctccgccgga tgaacttcgg cagtcgttcc
      121 gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc tgaaccggct tcagaaagag
      181 agcgatacgc tgactggtag ggtgtatgac ccggcagtac agcgtccgtt gcgtcagacc
      241 cgcacccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact gttgcctgcg
      301 gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga taccgccgaa
      361 cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga aaaacatgcc
      421 tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat cgagcggggt
      481 atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga gcacaccccg
      541 ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg ttcactgctg
      601 tcgggctggg tggatgcatg ctgccggctg ctgtctccga tggaagaggc gcttcatggc
      661 tatgtgctga ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt gctgttgcca
      721 ggcaataaga aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga ccgtaacgcc
      781 gggtcaacgc tggcgccggc ggtgtggttc gcttacagcc cggacagaaa aggcatccat
      841 ccgcagaccc atcttgcggg gttcagtggt gtactgcagg cggatgcata cgccgggttc
      901 aacgagctgt accgggatgg ccggataacg gaagccgcct gttgggctca cgcccgccgt
      961 aaaatccacg atgtgcacgt tcgcaccccg tcagccctga cggaggaagc gctgaaacgg
     1021 atcggcgaac tgtacgccat cgaggcagag ataaggggaa tgacggcgga acagcgcctt
     1081 gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg gctgcgtgaa
     1141 aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata cgccctgaac
     1201 cagtggccgg cgctgacgta ctatgcagat gatggctggg ctgaggcgga caataacatc
     1261 gctgaaaatg cgttgcggat ggtcagtctg ggccgcaaaa actacctgtt cttcggttcg
     1321 gatcatggag gagagcgggg agcgctgctg tacagcctga tcgggacgtg caaactgaac
     1381 ggagtggagc cagaaagcta cctccgctat gtccttgacg tcatagccga ctggccgata
     1441 aaccgggtcg gcgaactgct cccctggcgc gtagcactgc cgactgaata acacattccc
     1501 gtcaatacgg ttcttgctgc acgcttacaa acaaaatggg ttggctaaag ctctgagaga
     1561 gattggccgt atagaacgaa cactgtttat gctggactgg tttcgggatc cggcattgcg
     1621 gcgacgtgtc caggccgggc tgaacaaagg agaagcccgt aatgctctgg cccgtgctgt
     1681 attcatgcat cgattggggg agattagaga tcggaaaccg gaaaatcaaa gttatcgcgc
     1741 cagtggattg actttattga cggcggctat ttccttatgg aacacagttt acatggaaag
     1801 agcggttgat gctctgaagc gtaagggcat gaaaataaat gctcaactat tatcgcattt
     1861 atcaccatta ggttgggaac atatcaatct gacaggcgat tatatctgga aaaacagccg
     1921 gataccgact tcaggtaagt ttcgtcgttt gagatcagtt aagatcgaca aggtcaaaaa
     1981 acaaccttaa cgtacaataa ttttcgatat ccaaactgac cccaagtata aaaaacagcc
     2041 tgtcacggta taaaaatcga ccatgcagaa attatgctgg ttattttacc gctgctggtc
     2101 cctcattcag tgactgcccc aggggttttc atgctctcct tatagtacaa taatttccat
     2161 atccaaactc acccctactt tgacttttat catagtcaaa acctcttaac gtgagttttc
     2221 gttccactga gcgtcagccc tctaaaacta ctgaataatc agttagaaaa caaacaatcg
     2281 gaaaaatctg gggttccggc ctagaacccc atgaacaggc atagctgtga tttcatttaa
     2341 ttattggcac aaatcagttg atcgtacaat gctgtgcctg tctcgttttc tggagattcc
     2401 tcagcctcat ggagcggcgt aacaggcgta ccggcaggac agagatgttt gcttttccta
     2461 gtccggccag tttagtggaa taacggagtt tttatcccat gttttttccc cccgatcacg
     2521 actttttcaa atcaatgctc agaatacgtt ctttagctcc ttccacaaac acatcaggat
     2581 cccaatcgcg gttaaagcct tctttgatgc caatgtaaag ggtatccata tcgtccgaca
     2641 acaccatctg gtttgggtag accggcatgt taaattcacg ggtgatgctg tatttggtgg
     2701 catccactac gctgatggtg ccgctagtga agttggtgac atacagttcg ttatatttcg
     2761 gattgtattt caccgcgtta gctccggctc ctgtgtgcac atagccaagc tgtttaccgt
     2821 cgcgtaagtc aaaaatatag agtccatctt ttgtaccgga acgatcgtca tggtcgaata
     2881 ccaccacaaa ggcacggtca gaagcggagt ccaccgccag attctgcaca cttgtgattt
     2941 gtggtctcgg atctttcaac gggatggtac gcagcagcgt ctgatttttc gcatcaattt
     3001 cgttgatgta atctcttccg ccaacgtaga ttttctgtgt tttttcatcc atcgtcagac
     3061 catacgctgt acctttgtaa gcatccttaa cctcgccttt taaggcaagc gtcgtcccgt
     3121 cgagcattaa cagcttatgc agaggcttca tgccctcggc cgttttcagc atattgctat
     3181 aactcacaaa cagggtatcg gaatcctggc tgtaaaccat atggcgaaaa tgctcaaaac
     3241 gcgaatctgc agcgtttgcc gctttaaaac ttaacctgtc gctggttctt accagtttgc
     3301 cggttggggt gtcaaacata ctgatacgca gggacgctga ctgggtgtgt ccgatataga
     3361 gctgatgttt atcctgattt atcgccagcg aaaatgcccg gtatggcatc gtgtatttat
     3421 tttcgacttt cagcgaatct gaattaaacg aatacagata gcctttattg gcttcacgat
     3481 ttacacgatc ggtgactgct gcaaacacct ttttactggc attatcaaac gccagttcat
     3541 agacgccttc atcaggggac tgaaggtaat tcgttttcag ggcaaatcgc gaaagttcgc
     3601 gttcaggtgg atcgatagcc atggccatcg tgggtaaaaa tagtgaaatt aacaggtata
     3661 gcggtgcttt ggataaagtg aaaatattca tttttcgttc ctgcaataaa attgacataa
     3721 gagagaaaac gtattgtctg aacctagcgg aaattgtcat cagaaacgaa tgtccccgcc
     3781 aaatatccat gttctgccac gcgcggaatt ggctgggtgg gtctcgtcac caagacctgg
     3841 catcatgttg agcttattca gcgcctccga ataatctctg ttcatcaggt tttgtaccga
     3901 aagtttcagt gttaggttgc ggttgtactc gtaagtacca tagagatcga taatcgttgg
     3961 gatctgcggc aaatcctgtt tgattattgc gccggtatgt tcgtcctgct caaaatcagg
     4021 cgacaggcga cgagccttgc ctgtgtattt tatgatagtg cccagggtca acgcgttatc
     4081 gaagaagcga acaccagtat ccagcgtcat gtattttctg ggcagttcgg tcatatcccc
     4141 tgcgccaaag taggtgctgg cgatggaggt tggctggtct gtttgctgct ggctgaaaga
     4201 gagtcggcca aaagcaaaac ctgcatcata atccatctcc agttcgaagc cctttgcgat
     4261 aacgtcgctt gccgagttaa cgtagatgta catattgtcg ctgtattcat cgctaatgcc
     4321 ctcccagtca ttgccaatca cctcaggcat actgcattta cgacctccag aacaaaccag
     4381 ataagactcg ctgtagatgt agttctggat cctgctgtgg tacgccagag ccttaaagcg
     4441 cagggcatcc tgttcgacca gtaaatctct ggtatcaatg ttaaaacccg cctgccaggt
     4501 ttctgcacgt tcaggcttca ggaatgggtt catggatgcg cctcctgaat tagagaagaa
     4561 catctcctgg atgttcgggg cgcgcatgga tttgctgtag ccaatgaacg gctgaagcca
     4621 tggtgttacc tgagcagaaa gctgaactga agggttgaag ccaccctctt tatcgtcaat
     4681 atcgtagcta ccctgtggca cgcagataac gcgtgaatcg caggcgggct tgtaccctgt
     4741 gatcctgtta cgtgtgtagt tgagattgaa atctgcctcc cagatgccgc gcgtaacctt
     4801 cagcccggta tacagcgctg aaatatcctg ctggccggag ggtgcgaacg gattgttctc
     4861 gatcgattcc tgattcgctt tcgggtcgcc cgccgctgag tgaatggtcc ggtcatagcg
     4921 ggtacgcatc agtttgctgc ccagcataaa ctccaggtca ttgtccgcga cagtgaaccg
     4981 gctggtattg ttgatatcca gcgcgtcaga acgattttgc gctgaggttt tgtagaaagt
     5041 atacagcgag ccatcacgat acttttgatt accgcgactg gtactggccg ttacgttaaa
     5101 gtcaattaat tcagaaaaag gggtgtaatg atatttaatg taatagtcgt cactggttat
     5161 atcacgacgt gtaaatttat tttcataggt tcgagcggaa agttcgaagc tgttaaattc
     5221 gtctggtctg atatccattt tatacagttg ggatttgggg ttctgcttca tatatttatc
     5281 ataaccaaac tctttgctgt taattccaga accatttgag aaattagagt acacagaact
     5341 gccgctcaca gcagccataa cgccaatgct tcccgtatcc gtaaaagcgt cgctttttcc
     5401 accaagggcg atcattccgc tgcgtcccag cccattacta ccgacagaga aacgtgaacg
     5461 aaggccatat gtattaccgt taaatattac atcgtcaacg ccaatagtac gcatatttgc
     5521 actaccggca agggcgttga tcccttcaga gccactgctg tcaccgcgtg taacatcaac
     5581 tgctaccagt aagttaggat ctataagtac gccagccata ttgttagttg aaccatgcgt
     5641 cgttgttccg gaggtagagg ttccgtaaaa actctgggta ataccatcga ccatagtgtt
     5701 tacacgacca aatccgctca tgcctcgaat attcacactg attgctccct gaccaggatc
     5761 tatttgagta taagtaccag gcatactacg cactgttgcg tcaagtgatt gcaggttttt
     5821 gtccgttgtc cgggaactgg tcgcgcctgt tttctcgaga gcttcctgct cactggtcgt
     5881 cttcccgaca gaaacgctga gcggactgaa ttctgccgct ccttttttgt cggaattagt
     5941 tttttcactg atggattgtg agtaagcacc gcagctgagc aataatatgc ctgagccgat
     6001 agccagtttt ataacgttca taaatt
//
LOCUS       DAZPAC010000234         6015 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZPAC010000234 DAZPAC010000000
VERSION     DAZPAC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.235"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(369..1304)
                     /locus_tag="ACHUPM_003777"
     CDS             complement(369..1304)
                     /locus_tag="ACHUPM_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0871439.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDLNAPAPNITIKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLS
                     TSARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTAETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
     gene            complement(1802..1945)
                     /locus_tag="ACHUPM_003778"
     CDS             complement(1802..1945)
                     /locus_tag="ACHUPM_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFR0871440.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGV
                     ETQ"
     gene            complement(2029..2286)
                     /locus_tag="ACHUPM_003779"
     CDS             complement(2029..2286)
                     /locus_tag="ACHUPM_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="HFR0871441.1"
                     /translation="MGLKLHIHWFDKKNEEFKGGEYSKDFGDDGSVIERLGMPLKDNI
                     NNGWFDVEKRWVPILQPYFKNVIDISKFDYFVSFDYRDGDW"
     gene            complement(2296..3933)
                     /locus_tag="ACHUPM_003780"
     CDS             complement(2296..3933)
                     /locus_tag="ACHUPM_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase
                     domain-containing protein"
                     /protein_id="HFR0871442.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENN
                     PWGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVS
                     GEALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTN
                     VQVSTLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASF
                     PGVPSLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKP
                     VYVSVTDVLTPAQVKQRQDEEKRLQQEWNDAHPVEVAERNYEQARAELNQANKDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQ
                     RAQTDVNNKKAAFDAAAKEKSAADAALSSAMESRKKKEDKKRSAENKLNEEKNKRRKG
                     TKDYGHDYHPAPKTEDIKGLGELKEGRPKTPKQGGGGKRARWYGDKGRKIYEWDSQHG
                     ELEGYRASDGQHLGSFDPKTGKQLKGPDPKRSIKKYL"
     gene            complement(4154..4687)
                     /locus_tag="ACHUPM_003781"
     CDS             complement(4154..4687)
                     /locus_tag="ACHUPM_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871443.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQRLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(4690..5616)
                     /locus_tag="ACHUPM_003782"
     CDS             complement(4690..5616)
                     /locus_tag="ACHUPM_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR0871444.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTEPERVKSPSRGFSMGF"
     gene            5792..>6015
                     /locus_tag="ACHUPM_003783"
     CDS             5792..>6015
                     /locus_tag="ACHUPM_003783"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871445.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEG"
BASE COUNT         1487 a         1660 c         1297 g         1571 t
ORIGIN      
        1 aaaaaaacaa cgacgagaac gcaatcaccg actctttctc gcggctgggc tactgatcga
       61 agcaggactg gtagacacaa aaacaggcga gctatgctat aaaaaagaca ggatattaca
      121 ggcacttaag gaaattaagt acgatctgga gacatcccca aacccagacg cgtaagtgcc
      181 tccatccgtg ttcctgttcc ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat
      241 gagactgcgg cgagcactac cagcacaatc atgcggaaaa cagcaccatt gcagggctga
      301 acggggaaaa acggcaaaag acctaaggct caaaaaaacg ccagaaaggc gctgttatct
      361 gatataggct attttccgct tttccagttt cgaatggttc gagtagaaac ctccaacatc
      421 gccgcaatag ctttctgaga atatccattc tccagcatac atagcgccat gaaacgcttg
      481 tcatcgtagg cttcgccttt agcgatgcca cctttcctgc ctctcttggc ctgtatttct
      541 ggcgtgtgcg tatcagccac atactgtgcg aaagtttccg ccgtgaagtt cctgtgcgtg
      601 tatttcgcaa tactcttgcc aatagcccga cattcaggag gtgaaagcgg aacgggaagc
      661 gatgcgttgt acatttcgac acgctgaatc acggcatcaa gccactgtga gaatgctggc
      721 cagccctgac gaatcgccct gtaagcccat ttgcgcgtca tttcgaacag atggcagttt
      781 ctccccattc cgtaatgttt atcgatgcta cggcgcgctg aagtgctcaa atcgagataa
      841 tcagccagtt catcgagggt ataggcttcc tcacgccact ccatcacctg ccattccaga
      901 tggaacggat ttttgcaaat cagtccgctg tagtttacat ctgcgcccag tttttcacac
      961 aacgcacgtt caatcgcggc ggcgtatttc agcgccttaa ccgaagcatc aggcgcggtt
     1021 ctcacggcga tattgagtgc gtagagcagg tgggcgtgac cgttcacggg attttttatg
     1081 gtgatgttcg gtgcgggggc gttcaggtcg ctccagtcaa tcgcagcccc ctcgcggtca
     1141 acgtcaaaaa ccagccagaa ctgcgcatga ggttggttct gctggatata ccgtgcaaga
     1201 agcgcgcgcc ctttaccgga gatacgaagg ccgaaagcga gatcatccgt gtgatagggg
     1261 cggaggggta aattttcttc gaagtattga agcgcggcgc tcatgtgtta cgattccttc
     1321 caggttatcg accgacctcg cccctgtatt ctcgtgacaa tcttggcgga gagacacgaa
     1381 tttcaggggc gtttgttttt cagtcgctta cactacaccc aacggcaaat cctgtcgatc
     1441 cttcccgaaa acccgatcca tttaccataa ctcccgttgt acgtacccct taaaacgccc
     1501 tcaatcgctt tctggcgcaa aaaacgcaaa aagacaagaa aacaccagga caaaccagaa
     1561 aaacgcgtca gaatcgtttt cagggcgttg cataactatg cacgaaagcg atgtgcgatc
     1621 ggtttcgtgc gttctgaaca ccgaacggct caaaacgaaa agttttctcc ctccggatgg
     1681 aaggttctgc acggagattt tagatcccat ctcccacatg gaatttttgt ggcacttcaa
     1741 caaaaaaagc gcgtcagaat cagcctgaca cgctttgtcc ttgtcaaatg aggattgcag
     1801 gttactgcgt ttccactccg gtcagttcag cagaggatga cggtgataca gtcccgccct
     1861 gaacatcacg gatatagttt gcctgacatg cagcaagaat aatggctgca agaagcaaca
     1921 aaataatccc tgttattttc ttcatacgat tccctgtata tatggattca ggaattcatc
     1981 cttgaacacc tgaatcaaac actttattac tcgtccctga tagtttaatt accagtcacc
     2041 atcacgataa tcgaaagaaa caaagtaatc gaacttacta atatcaataa catttttaaa
     2101 gtatggctgt aatattggca cccatctctt ttcaacatca aaccaaccgt tattaatatt
     2161 atcctttaaa ggcattccaa gacgttcaat aaccgaacca tcatcaccga aatcctttga
     2221 atactcacca cctttaaatt cctcattctt cttatcaaac caatgaatgt gtaatttaag
     2281 tcccataaca tcaccttaaa gatacttttt aatacttcgt ttcggatctg gacctttcaa
     2341 ctgcttaccg gttttagggt caaatgaacc aagatgctga ccatcactgg ctcgatagcc
     2401 ctcaagctca ccatgctgag agtcccactc ataaatttta cgccccttat ctccatacca
     2461 tctggcacgc ttacctccac caccttgctt tggagttttt ggcctcccct cttttaattc
     2521 ccccaaacct ttaatatcct cagttttagg agctggatga taatcatggc cataatcttt
     2581 agtgcctttt cggcgtttat ttttctcttc gtttaattta ttttcagcac tccttttctt
     2641 atcttctttc ttcttcctgc tttccatcgc agaactcaat gcagcatctg ctgctgactt
     2701 ctcttttgcg gcagcatcaa atgcagcctt cttattattt acatccgtct gtgcccgctg
     2761 ggccttaagc cctgccattt gccacattct gtgaccagca gccattggtt ctcgggcaaa
     2821 tcgctcgaat tgttttattt cagcctttgc atcagcaaga gttttattcg ctgcatcaag
     2881 ttcactttta cgagaattat aaacctgaac agctttagcc tgtcgttcct ggtttctggc
     2941 aacatcttta ttagcctgat tcagttctgc gcgggcctgt tcataattgc gttcagccac
     3001 ttccaccgga tgtgcgtcat tccattcctg ctggaggcgt ttttcctcat cctgacgttg
     3061 ttttacctgt gctggggtaa gaacatctgt cactgataca taaaccggct tctgcccact
     3121 ttctttcgga aagcgaatca ccgcttcatg tgaaccacca ccaaaggtaa atcccgcagg
     3181 aacggcagtc cggcctttat cctccgtaat accacgggga agggttgttg acaccggaag
     3241 gcctttgaca gtgctgactg tcagagaagg gacaccagga aatgatgcgt gaaatacacc
     3301 tggagtacgt gttggtttgg cattcacaac aggcacactc ataggcacac ctgcaactac
     3361 ggcaatatgc tgtcgtgtgt ccttcacaac atctgtcaca cgtttcgtca cgctgacagt
     3421 tgcctggtcc agcggaagtg tactgacctg aacattcgtc actgtttctg ccggtaatga
     3481 cgtcacaatc tttgacatca tattcgggtc atcttttgct atttcagatg gcagaatgcc
     3541 gtaaagcgca ataccccatg cgctgaattt aaacgggcct ttcagggctg caaagatatc
     3601 cgctattgcc gcagataaag cctcaccaga gacggcgata cccagcgttc cggcaccagg
     3661 agttgccaaa gcaggaaaac caaatgccac tgctgctacg gatgaattgc tgccaccacc
     3721 ggaattacca ttccccccgc cattaccatt gccggagcca cctccccagt gaacaccact
     3781 accggaaccg ccaccccatg ggttattttc cgaactccat cctgaaccgt cagaagcccc
     3841 accatttcca ccaagccccg tagggccgcc attaatgtta ccacctgtgt tatgtgcgcc
     3901 actgttgtgg tcgcgtccat ctccaccgct cataaaaatt ccctctttta agcgttaaaa
     3961 caattgatta tatagttgat actgtacata aaaccactgg ttttatgtac agaccaaaaa
     4021 gtactcgcca ttattttttc tgtcaagtca gggggggata ttttttggca gttcggagga
     4081 atcactcatg aaaacggtga acggaacgcg aaatcctttc gcgggtcgtg aggcgttgga
     4141 caagcgaagc gcgtcagttt ttccggctct tacccgtgac aggtttctcc accagtttat
     4201 cttccagtgt cacaactttc tgatacaccg ccgctgtggc gttgtagttc ctgtcgagaa
     4261 ttgttttcat cccccatccg agcacactga acataaccag aaaggccact gacaccccac
     4321 ccagccattt ccagctatac cctttaaggg tgttgtattc ctgcacgata ttgctgtgaa
     4381 ttgtcgcaat atccctgttc agggcatccg cgtttgcctt catgtttgcg actgactctt
     4441 ccagccgctg aaaatcctga ctgatgcgct cacctatcag ccctgcaaca tccagcctct
     4501 gcacttcgtt tctgacttca tccctgacct gttttgccac accgtcagaa atatcccgat
     4561 atccgcgaag tgtttccctt gccacagcca gagaatcagc aacagctttc tgttgctcca
     4621 ttgcggtatg ttgcagatgc tccaccgata acaggagcgt gagtaatttt tcttctgtgc
     4681 tggccatgtt cagaatccca tagaaaatcc acgtgacggt gatttaaccc gttcaggttc
     4741 agttcggttc tgtctgctca ctctgtctct catgtcaaag ccatgctccc gcgcaacatc
     4801 atgttgcagc cttctgacca gttcatgttg cttaaccgga gtgtccagcc attccctgat
     4861 gtcatgcccg accgttcgcg cctgttcaag atggcgggaa tagtccggtt cagaccgttt
     4921 ttgcatgatg tcatgacgct gggcatcatc aagccgtccg gcagcttcaa aacccagatg
     4981 cacttcggga cgtcgttcaa tgccctgttc tttcagcgtc cggcagtcaa tgaagcccct
     5041 ttcaaagcca tgctttctca tatgttcgtt gtgcttcacc tcccagcgtt ttcgcaattc
     5101 ccgcaactgc tctttttgct gagtgagagt caggcttccg gaatcctttt tcgcacctcc
     5161 ccgctcggga tacctggcgt tatagcgccg gaaatactgt tccggtgtac gttcaatgcc
     5221 atcattcaca cgctggctca tcatgatatg cgcatgaggc tgttcgccgc cgtcaatgct
     5281 cgctttcgga ttgtgaatgg caaaactcca ggcgtgccgt tcgccagctt cctgacgtac
     5341 aaaatcctgc accagttcaa ggcgctgttc cgctgtcagt tcacggggta atgcaatttc
     5401 aagttcccga taggtcgaac cgttcgcacg ttcaaactgg tcagccgcct gccagaaatg
     5461 tgagggatta tctcgcgccc attccggcat gtttccgtgt gcggaatatt ccaaatcctg
     5521 acggtcgcgg tatttctctt tgcgctcgat gtaatcggcg tggtttgcag cctgcccctt
     5581 gccaccaaat ttaacgctga ggtgataggt tgccacggtt tcagccctcc gcaggagcgc
     5641 actgtttctc gacgaaggag agaaacatat aagtgcgctt ccttctaatt ttacgaatta
     5701 gcacggaaga taccccctgc acccaccgac cgcaagcgat atactgcacg accacaacac
     5761 acagcaaaaa acaccaggga gcaccacgaa catgcgcatc agcaacatcg aatggctgaa
     5821 gaaacggatc ggattcatca ggaagctcgg agaacagacc gcacgacaga gacagattat
     5881 tgacctgctc gacaacgaag caggactcac agaacaggaa cgcaaactcc tccacgtact
     5941 ggcaacagcg gaaaaaaacg acttacaggc acaggagagc gaacgaaaac aggcggtcca
     6001 aaaacgaatc gaggg
//
LOCUS       DAZPAC010000235         5981 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZPAC010000235 DAZPAC010000000
VERSION     DAZPAC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.236"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(118..2262)
                     /gene="fadJ"
                     /locus_tag="ACHUPM_003784"
     CDS             complement(118..2262)
                     /gene="fadJ"
                     /locus_tag="ACHUPM_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR0871446.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(2262..3572)
                     /gene="fadI"
                     /locus_tag="ACHUPM_003785"
     CDS             complement(2262..3572)
                     /gene="fadI"
                     /locus_tag="ACHUPM_003785"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR0871447.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(3752..4036)
                     /locus_tag="ACHUPM_003786"
     CDS             complement(3752..4036)
                     /locus_tag="ACHUPM_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR0871448.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            4408..5748
                     /gene="fadL"
                     /locus_tag="ACHUPM_003787"
     CDS             4408..5748
                     /gene="fadL"
                     /locus_tag="ACHUPM_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR0871449.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1468 a         1623 c         1436 g         1454 t
ORIGIN      
        1 tccattcacc gaatgaaacg ctgtttttta cctcaatggc gtaagtatag tcaatatgcg
       61 tttacatttt gaccaacact ccgccattca gcgcggattc atatagcttt gaccttctta
      121 ttgaaggtca gttgcagttg ttttccaaaa actttcccca cgcgcgccca tctcgaccaa
      181 acgctcgcaa ggggtaaaac gggaaccata ctgcgtggcc agtcgttgca ttattgcaac
      241 cacttcgccc gcgccgagag aatcgatata gcggaacggt ccaccgagaa atggcggaaa
      301 accaatgcca aataccgcgc caatatcccc gtcacgcacg ctacggataa cctgctcatc
      361 aacacaacgt actgcttcat tcagcatcaa catcacacac cgttcagcaa cctgcggtgc
      421 ggagagtcgc ccctgccctt gtgcgccaat gagcgggtaa atggcgggat cgacctgttt
      481 tttgctttta cgccctttct gaccataaag atagaaaccc cgaccatttt ttctgccttt
      541 gcgatcgtcg ttcaaaattg aagaaacaac atttgcaggc gcgctaaaac gttctccata
      601 agcggcttcc agtacaggaa taattttagt cccggtgtcg attcctacct catccaaaag
      661 ttggattggg cctaccggaa aaccaaattt cactagcgcg gcatcaatgt gctctacccg
      721 ttcaccttcg gtcagcatgc ggatagcttc attaatgtaa ggcgctaaga tgcgattgac
      781 gtaaaacccg gctttgtcac gcacgacaat gggcgttttg ccctgttttt tcgccagttt
      841 taccgtagtg gcgatggttt gcgccgatgt acccgcatga ggaataatct ctaccagcgg
      901 cattttttcc acaggactga agaaatgcag gccaataact tgctcaggtc gcgcggcgtg
      961 agcggcgata tcaccaatcg gtaaagatga cgtgttcgaa gcaaaaatgg tatgagcggc
     1021 gcaattttgc tccacttccg ccaccatctg ttgttttaat tcgagatttt caaacaccgc
     1081 ttcaataatc agatcgcgat gggcaaagcc gcgatagtcc gtcgttccgg agattaatgc
     1141 cagctgtttg tcacgttcgc tggctttgag atgacgacgg cgaactttgc cctccagctg
     1201 atcccaactg tacttcagcg catgatttat gccctgcggg ttgatatctt taattctgac
     1261 cggaagcccc gctttacaag cagtgacata agcaataccg ccgcccatca agccaccacc
     1321 taaaattccc acgctgttta atggcgcagg cggtgcatca ctaccgggat ctttcttcac
     1381 gtccgtactg gcaaaaaaga tactacgcaa agcttgcgat tgcggcgtca tcgccagttc
     1441 gccaaaggcg cgagcttcag cgtcatagcc gctgctggtg ccctgcgcta atcccgtttc
     1501 aacaacctcc aggatgcgtt ctgtcgccgg ataattgcct tgagttttgt gttctgtttt
     1561 cttgccgacc attttgaaca gcagcgcacg acctaacggc cccgccagaa tacgctcgcg
     1621 tacaggtaga gggcgggaag atgggcgatc cttcttcgcc agctcaacag cggcttccag
     1681 caaaatggag tgcggaacaa cgtcatccac cagccccagc tttaatgcct gtttcgcccg
     1741 aagttgtttt ccggtgagaa tcatctctaa tgctgtactg acgcctatca gacgcggtaa
     1801 acgctgggtg ccgcctgaac cgggtaacaa tccaagttgt acttcaggca aaccgagcac
     1861 cgttttagga tcgtcagtac aaacgcgacc gtggcacgcc agcgccagct ccagcccacc
     1921 gcccaggcaa gcgccatgaa tagccgcgat aaccggaatg ggcaaagcat gaatctccgc
     1981 catcaactgt tggccctgcc gcgccagtgc ttccgcttct tgcgccgttt tgcagttgcc
     2041 aatcatgttg atgtctgctc cggcgataaa attgtccggt ttcgcggaga taaacaccac
     2101 gccgcgcaac tctttgtttt cacggagttg cttaataatg gcgcgcacct gcgaggcaaa
     2161 ttccgccttc agggtattca ttttctcatc cggtacatcg atggtgatga tagcaatgtt
     2221 gtccagacga acattaagag taaacgctga tgccatttcc attattccgc ctccagaacc
     2281 attgccgcgc caagcccacc ggcagcacag gcggtaacta aaccaaatcc accgccgcga
     2341 cggcgaagtt catgcaatgt ctgggtaatc atccgcgcgc cggtcgccgc gaagggatgc
     2401 ccgtaagcaa tcgaaccgcc aagcacgtta aatttgctat cgtccacttc gccagtggca
     2461 tgtgcacgcc ccagtacttc acgagcaaaa cgttcactac ccagcaactg aatattcgcc
     2521 agtgtctgag ctgcaaaggc ttcgtgcata tcgatcaatg tcagttcgct catcgtcaaa
     2581 ccggcacgtt ccagcgccag cggtgttgac caggctggac cgagcaacat gtcctgccag
     2641 acatcaatcg cagtaaatgc gtagctgcgc agatacccca gcggcaccag ccctaattct
     2701 ttcgcccggg attcagtcat caggatcacc gctgccgcgc catcggtcag cggcgtactg
     2761 tttgccgccg ttaccgttcc gtgtttgcga tcaaacgccg ggcgcagctt tgcgtaatcg
     2821 gcaagcgagg aattaccgcg aatattgttg tcttcgacaa gcggttgttt ataaggaggg
     2881 ataaaggcag tcatcacctc ttctttgagt tttccgtctg accatgcctg agcggcacgc
     2941 tgatgcgaac ggtgcgctaa tgcatcttgc tgttctcggg tgatgccgta ggttttcgcc
     3001 atttgttcgg cggtgtcgcc catccgcaag ccagtagaat attctgctac cgcaggcggt
     3061 acgggcatta agtcgcacaa acgcaggcga gagaagagtt tcaggcgctg gctcatggtt
     3121 cgagctttgt tgacatcaac cagcacgcgc gccagttttt tactgacgcc aattggcaat
     3181 acggaagagg aatctgcccc accggcaatc cccgctcgga tagttcccgc catcaggctt
     3241 tctgcgacgt ttgcaaccgc ctggaaactg gtagcgcaag cgcggctgac gctgtaagca
     3301 tcggtatgca cattcattcc ggtaccgaga acaatttcac gcgcaatgtt gggggcttca
     3361 ggcatttgta cgacctgacc aaagaccagc tgttcaatca cttcagcggg gatctcgctg
     3421 cgtgccagca gttcgcctac caccatcttc cctaaatcaa ccgcgggaat gccatgaaaa
     3481 gccgtcgcct ggcgggcaaa aggcgtacgt aaaccgctaa caatggcgat acgatcgccc
     3541 tggcgggtaa ccagcggtaa aacctgaccc ataacactcc cctgtaaaaa aataaataaa
     3601 gtggtctgac ctgatcatag tcttaactat ttttttacat ttagccaagc ggagaaaaag
     3661 gaaagtggga gctatgacac agtgaagaca ggagaaagaa aagaaaacgc ccctgccagc
     3721 aactgacagg ggcgaacgta cccgagagga attaacgcag acccagctgg aagatcagca
     3781 tttcagcttc gcaggcaaaa gtgaaatcga tatccaggcg cacaccgtca gactcttcag
     3841 tgaaagtcgg ggagattttg cacggttcgg attccacgct acgggctttt tcagtcagcg
     3901 ccgccagcgt ttgttctgct tctgcgcggt ttgcaaacac gcggctgtaa gacgcggtgc
     3961 agtcggagtt gtccataatg gtgccaacat ccatacagca gcaaaccggg gtttcatcag
     4021 cactacattt actcatcgtt gatttcctct gtatgtgcac ccaaggtgcc agataaacgt
     4081 tgtggatatt ttacgcttcc ggaaagtgct gctccagttg ttaattctgc aaaatcggat
     4141 aagtgaccga aatcacactt aaaaatgatc taaaacaaaa ttcacccgaa tccgcgagtg
     4201 cgcaacctcc aaattttgcc agctggatcg cgtttcttag atcatatttg aaaaaagatg
     4261 gaaacatact tgcagcattc cagctggtcc gacctatact ctcgccactg gtctgatttc
     4321 taagatgtac ctcagaccct acacttcgcg ctcctgttac agcacgtaac atagtttgta
     4381 taaaaataaa tcattgaggt tatggtcatg agccagaaaa ccctgtttac aaagtctgct
     4441 ctcgcagtcg cagtggcact tatctccacc caggcctggt cggcaggctt tcagttaaac
     4501 gaattttctt cctctggcct gggccgggct tattcagggg aaggcgcaat tgccgatgat
     4561 gcaggtaacg ttagccgtaa ccccgcattg attaccatgt ttgaccgccc gacattttct
     4621 gcgggtgcgg tttatattga cccggatgtg aatatcagcg gaacgtctcc gtctggtcgt
     4681 agcctgaaag ccgataacat cgcgcctacg gcatgggttc cgaacatgca ctttgttgca
     4741 ccgattaacg accaatttgg ttggggcgct tctattacct ctaactatgg tctggcaaca
     4801 gagtttaatg atacttatgc aggcggctct gtcgggggta caactgacct tgaaaccatg
     4861 aacctgaact taagcggtgc gtatcgctta aataatgcat ggagctttgg tcttggtttc
     4921 aacgccgtct acgctcgcgc gaaaattgaa cgtttcgcag gcgatctggg gcagttggtt
     4981 gctggtcaga ttatgcaatc tcctgccggg caaactcctc aagggcaagc attggcagct
     5041 accgccaacg gtatcgacag taataccaaa atcgctcatc tgaacggtaa ccagtggggc
     5101 tttggctgga acgccggaat cctgtatgaa ctggataaaa ataaccgcta tgcactgacc
     5161 taccgttctg aagtgaaaat tgacttcaaa ggtaactaca gcagcgatct taatcgtgcg
     5221 tttaataact acggtttgcc aattcctacc gcgacaggtg gcgcaacgca atcgggttat
     5281 ctgacgctga acctgcctga aatgtgggaa gtgtcaggtt ataaccgtgt tgatccacag
     5341 tgggcgattc actatagcct ggcttacacc agctggagtc agttccagca gctgaaagcg
     5401 acctcaacca gtggcgacac gctgttccag aaacatgaag gctttaaaga tgcttaccgc
     5461 atcgcgttgg gtaccactta ttactacgat gataactgga ccttccgtac cggtatcgcc
     5521 tttgatgaca gcccagttcc ggctcagaat cgttctatct ccattccgga ccaggaccgt
     5581 ttctggctga gtgcaggtac gacttacgca tttaataaag atgcttcagt cgacgttggt
     5641 gtttcttata tgcacggtca gagcgtgaaa attaacgaag gcccatacca gttcgagtct
     5701 gaaggtaaag cctggctgtt cggtactaac tttaactacg cgttctgata acgcgttcgc
     5761 ctggataaag tcacctgcat agcaggtgac tttaactccc ccacttcacc gaaagtagtg
     5821 tcctcattgc ttacctcctg agttttgcaa acagcctgtt ggcagcttgc cctttcaaat
     5881 caataagcgg tatccacaga caaacctgaa gggaaaggca tattttcagg cgttctgctc
     5941 gtccttctca aagagtttac ttttctgcat ttccaggata c
//
LOCUS       DAZPAC010000236         5961 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZPAC010000236 DAZPAC010000000
VERSION     DAZPAC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.237"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            292..368
                     /locus_tag="ACHUPM_003788"
     tRNA            292..368
                     /locus_tag="ACHUPM_003788"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHUPM_003789"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHUPM_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR0871450.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="ACHUPM_003790"
     CDS             complement(1357..2247)
                     /locus_tag="ACHUPM_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR0871451.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHUPM_003791"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHUPM_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFR0871452.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..5938)
                     /gene="nfrB"
                     /locus_tag="ACHUPM_003792"
                     /pseudo
     CDS             complement(5207..5938)
                     /gene="nfrB"
                     /locus_tag="ACHUPM_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
BASE COUNT         1354 a         1564 c         1556 g         1487 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagag cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg agttgttcgg
     5941 taatgactcc aacttattga t
//
LOCUS       DAZPAC010000237         5899 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZPAC010000237 DAZPAC010000000
VERSION     DAZPAC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.238"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..2199
                     /locus_tag="ACHUPM_003793"
                     /pseudo
     CDS             1..2199
                     /locus_tag="ACHUPM_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            2192..3328
                     /locus_tag="ACHUPM_003794"
     CDS             2192..3328
                     /locus_tag="ACHUPM_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR0871453.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="ACHUPM_003795"
     CDS             3325..5325
                     /locus_tag="ACHUPM_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFR0871454.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5447..>5899
                     /locus_tag="ACHUPM_003796"
     CDS             5447..>5899
                     /locus_tag="ACHUPM_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR0871455.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKE"
BASE COUNT         1366 a         1568 c         1608 g         1357 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgcttactaa
     5341 gagttctact ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc
     5401 ctggtgagag tcaccagttc cttgattaaa taaaatggag ttttacatga aggctttcaa
     5461 taagctgttt tccctcgttg ttgcatctgt tctggttttc tctcttgctg gctgcggtga
     5521 caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc actgaaattg ccattaccta
     5581 tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc aatttgcctc
     5641 cattggtgca accaccaaag aagatgctgc caagacactt gagccgttaa gcgccaaata
     5701 caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctacg cgcaggaaaa
     5761 cgtgactatc gatatggaaa aagtggattt taaagccctg cagggtattt caggaatcaa
     5821 cgtttctgct gaagatgcca aaaaaggtat cactatggcg caaatggaac tggtgatgaa
     5881 agccgctggt tttaaagaa
//
LOCUS       DAZPAC010000238         5867 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZPAC010000238 DAZPAC010000000
VERSION     DAZPAC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.239"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..619)
                     /gene="tolA"
                     /locus_tag="ACHUPM_003797"
     CDS             complement(<1..619)
                     /gene="tolA"
                     /locus_tag="ACHUPM_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR0871456.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAE"
     gene            complement(684..1112)
                     /gene="tolR"
                     /locus_tag="ACHUPM_003798"
     CDS             complement(684..1112)
                     /gene="tolR"
                     /locus_tag="ACHUPM_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR0871457.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(1116..1808)
                     /gene="tolQ"
                     /locus_tag="ACHUPM_003799"
     CDS             complement(1116..1808)
                     /gene="tolQ"
                     /locus_tag="ACHUPM_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR0871458.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(1805..2209)
                     /gene="ybgC"
                     /locus_tag="ACHUPM_003800"
     CDS             complement(1805..2209)
                     /gene="ybgC"
                     /locus_tag="ACHUPM_003800"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0871459.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            complement(2359..2652)
                     /gene="ybgE"
                     /locus_tag="ACHUPM_003801"
     CDS             complement(2359..2652)
                     /gene="ybgE"
                     /locus_tag="ACHUPM_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR0871460.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(2652..2765)
                     /gene="cydX"
                     /locus_tag="ACHUPM_003802"
     CDS             complement(2652..2765)
                     /gene="cydX"
                     /locus_tag="ACHUPM_003802"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR0871461.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(2780..3919)
                     /gene="cydB"
                     /locus_tag="ACHUPM_003803"
     CDS             complement(2780..3919)
                     /gene="cydB"
                     /locus_tag="ACHUPM_003803"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0871462.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(3935..5503)
                     /gene="cydA"
                     /locus_tag="ACHUPM_003804"
     CDS             complement(3935..5503)
                     /gene="cydA"
                     /locus_tag="ACHUPM_003804"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR0871463.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         1529 a         1606 c         1386 g         1346 t
ORIGIN      
        1 cttcagctgc agcagcttct gccgcttccg ctttcttctt cagtgccgca gcggctgcct
       61 cggctttttt ctgcgcttcg gctgcggctt tggcggcttc tgcttctgct tttttcttcg
      121 cgtctgccgc cgctttcttc gctgcttctt ccgcagcttt agcatccgct tcggccttcg
      181 ctttagcatc tgccgccgct ttcgctgccg cctcttccgc ttgcttctgc tttaactcgg
      241 cctgttttgc ggcttcttca gcctgctttt tctgttcctg agcagctaac cgctctttct
      301 caagttgctt caggcgttcc tgttcagccg cttgtttctc acgcagttct tcagcagcct
      361 gctgttcctt tatcttgcgc tgctcatcag aacgcttcgc gcttgattcc tggctttgca
      421 tgcgtttgta ctgctcaact accgcacctg aatcaaccat gacagcgtcg atggacgaac
      481 caccgccgcc tccggctgaa gcttctatat tctcatcgaa cgaactccag agcagcgccg
      541 caaataagat gacatgcagc actgctgaaa taattatcgc ccgcttgagc ttgtcgtttt
      601 gttcggttgc ctttgacact ctcggtttcc aaaaactgtt cgcctgttac ccgctctctt
      661 tcaagcaagg gaaacgcaga tgtttagata ggctgcgtca ttaaaccaac cgatttcaca
      721 cccgcactat gtaacaagtt cagtgcttta attatttcat cgtaaggcac atcttttgcg
      781 ccaccgatca gaaagaccgt tttcgggttg gccttgaaac ggctggacac ttccgccacc
      841 acctgctctg gtggtaaacg ctccaggcga tctttctcaa ccaccacggt gtactgacca
      901 ataccagaca cttcaacaat cactggcgga ttatcgttac tgctcaccgc ctgtgattca
      961 gtagcgtctg gcagatcgac ctccacgctc tgggtgatga tgggcgctgt cgccataaag
     1021 atcagcaaca gcaccagcag tacgtccagc aacggtacaa tgttgatttc ggacttgaga
     1081 tcgcgacgac ctcgtccacg cgctctggcc atggcttacc ccttgttgct ctcgctaacg
     1141 gtaaacgcct ggcggtgcag aatcgcggta aactcttcca taaagttgtc gtaattcagt
     1201 tccagtttgt ttacgcgctg gttgaggcgg ttataagcca ttaccgccgg aattgcggcg
     1261 aacagaccaa ttgcagtcgc aatcaacgct tctgcgatac cgggcgcaac catttgcagt
     1321 gttgcttgtt ttaccgcccc gagggcgata aaggcgtgca tgatccccca gaccgtacca
     1381 aacagaccaa tatacgggct gatggagcca accgtaccga ggaacggaat gtgcgtttcc
     1441 agattttcaa gttcacggtt catggagata cgcatagcac gcgacgcccc ttccactacg
     1501 gcttccggcg catggctatt ggcacgatgc aggcgcacaa actctttgaa cccgctgtaa
     1561 aatatttgtt ccgaaccagt cagattatcc cgtttcccct gactctcttg atagaggcga
     1621 gagagttcga ttccagacca gaatttatct tcaaacgctt cggcttcgcg cgccgctgcg
     1681 ttaagaatac gggtccgctg gataataatg gcccaggatg cgattgaaaa accaatcaaa
     1741 atcaacatga taagtttaac cagaaggcta gccttcagga acaaatcaag gatattcatg
     1801 tcagtcactg cttaaactcc gcgacaatag acttgggaag cgcacaaggc ttcattttga
     1861 gtgggtcaac gcaaacaacc agaacctctg cttcattcag caaagtattc tcggcgttga
     1921 caatacgttg cgtgaaaacc aaagaggtgc cacgcattga tgttatttca gtctgtattt
     1981 cgagcatatc gtcgagccgc gcaggtgcgt aatattccac cgtcatttta cgtaccacaa
     2041 aggcaacgcg ttcagccatc agcgcctgct gactgaagtg atgatgacgc agcatctctg
     2101 tgcgtgctct ttcataaaaa gcgacgtaac tggcgtggta caccacacca ccggcatcgg
     2161 tatcttcata gtagacgcga accggccatc gaaacagcgt tgtattcact ttacatcccg
     2221 gtaatgcaac aaaagttaga gcttttaaac gctgctacta tacgcgcgcg attgaggttt
     2281 gggaatgagg gcaagttaag ggagcgtaaa aatttatggg cctggagcgg cccataattt
     2341 tttaaagaaa attctgattt aaaagaagaa gaaaatcagc ccgacaatga gaacaatatc
     2401 ggcaagcaat gggcaaaaaa tcccttgcca aagaaccttc tgcggacgaa agcccacgcc
     2461 gtgaatcaca ccagcacaga cggcccacat cagcaataaa ccatgccaga tttccagttc
     2521 actggtcttc gcggcaaaac gagatgggtc ccaaaacata catcctgcta acagaagcgc
     2581 catcacgaag gaaagcgccc gcagggggcg cttgtccatt accgcataca aggtcgcgat
     2641 aatcttactc atcagatgtc ttcttgaccg gctttgcctg attcgacgtg ttcaagcgcc
     2701 agcgcggtga ttaccccaaa cgaacaggca agaagcgttc ccagaatcca tgcgaaatac
     2761 cacattttta gctccttact tagtacagag agtgggtgtt acgttcaata tcttctttgg
     2821 tgatacgacc gaacattttc cagtaacacc aagcggtgta gagcagaatg atcggtacca
     2881 gaaccaccgc aacccaggtc atgacgttaa gcgtcagctg gctggaagtt gcatcccaca
     2941 ttgtcagact tgcgttcatc atggtgctgg acggcatcac aaacgggaac attgcgatac
     3001 cggctgtcag gatgatgcag gccagcgtca gggaggagaa cacaaacgcc cacgcggctt
     3061 tatccatacg tgcagtcagg atggtcagca gcggaagaac cacacccagt gccggaatag
     3121 cccacagaat tggcgtgttg ttgaagttaa ccagccatgc gccagcttca cgaaccactt
     3181 ctttattcag tgggttagag gctgcgtaat ggtccattgt cgatttcacg acataaccat
     3241 cgataccgta catcacccat acgccagcca gtgcgaaaca gaccagtgtc accagcgcag
     3301 ccacctgagc cgttgcacgg gtacgcaggt gcagttcgcc cacggtacgc atttgcagat
     3361 aggttgcgcc ctgagtaatg atcatcccta cgctcaccac gcctgccagc aggccgaacg
     3421 ggttaagcaa ctggaagaag ttaccggtgt agtacagacg cagatattca tcaacgttga
     3481 acggtacgcc ctgcaacagg ttaccgaacg ctacaccaat taccagcggc ggaacgaaac
     3541 taccaatgaa gatgccccag tcccacatgt tacgccagcg ggtttcttca atcttggagc
     3601 ggtagtcaaa accgaccgga cggaagaaca aagacgccag cacgaggatc atcgccacat
     3661 agaagccgga gaacgcagcg gcatagacca tcggccaggc agcaaagagt gcgccgcccg
     3721 cggtgatcag ccaaacctgg ttaccgtccc agtgtggtgc aatggagtta atcataattc
     3781 gacgctcggt gtcgttacga ccgaggaaac gggtgagcat gcccaccccc atgtcgaaac
     3841 cgtcagtgac tgcaaaacca atcagcagaa cgccaaccag cagccaccag ataaaacgca
     3901 atacttcata atcgatcatt tgacgactcc tgtcttagcg tgccggctga gtagtcgtgg
     3961 aagactgctc aaagtgatag cgaccggttt tcaggctgct tgggccgagg cgtgcaaact
     4021 tgaacattaa gaacaattct gccaccagga acagggtata caggccgcaa atcagcacca
     4081 ttgagaagat gagatcgcct gcggtcagtg acgagttcgc cacagctgtc ggcagcactt
     4141 caccgatagc ccacggttgg cggccatatt cagccacgaa ccagcccgct tctacagcaa
     4201 tccacggcag cggaataccg tacagcgcgg cgcgcagaag ccattttttc tcgccaatgc
     4261 ggttgcggat gacactccag aaagagagcg cgatgattgc cagaagcagg aagccacacg
     4321 ccaccatgat acggaacgca aagtacagcg gcgctacacg cgggatggag tctttggttg
     4381 cctgttgaat ctgcgcttca gtcgcatcag ccacgtttgg cgtatagcgt ttcagcagca
     4441 gaccgtaacc gaggtctttc ttcatgctat tgaactggtc acgaaccgcc tggtcggtag
     4501 aaccagaacg cagttgttcg agcagagagt acgccttcat cccgttacga atgcgttctt
     4561 catgctgcac catcagctct ttcaggccga taaccggggt atccacggaa cgcgttgcaa
     4621 tgatgcccag tgcgtaaggg atctgaatcg caaatttgtt cgtctcctct tcctgatcag
     4681 gaatgccgaa cagagtaaag gcagcaggcg caggttgcgt ttcccactcg gcttcaatag
     4741 cagccagttt ggttttctgc acgtcgccca tttcgtagcc ggattcatca cccagaacaa
     4801 taacagacag aacagcagcc ataccgaagc tggcagcgat agcaaaggag cgtttagcga
     4861 aggcgaagtc acgacctttc agcatatacc atgcgctgat accgaggatg aacatcgcgc
     4921 cagtcacata accagacgct acagtgtgaa cgaatttcac ctgagcaacc gggttaagca
     4981 ccagctcgga gaagctcacc atctccatac gcatagtttc aaagttgaaa tcggacgcga
     5041 ttgggttttg catccagccg ttcgcaacca gaatccacag tgcggacagg tttgaaccga
     5101 gcgccaccag ccaggtgaca cacatatgct gaactttacc cagacgatcc caaccgaaga
     5161 agaacagacc tacaaaggtg gattcgagga agaaggccat cagaccttcg attgccagcg
     5221 gcgcaccgaa gatatcccct acatagtggg aatagtaaga ccagttagtc ccgaactgga
     5281 actccatggt cagaccggta gccacaccca gagcgaagtt gataccaaac aacttgcccc
     5341 agaacttggt catatcttta taaatctgtt tgccggagag gacgtagacc gtttccataa
     5401 tggccagcag gaacgccata ccgagcgtca gtggcacaaa aaggaagtgg tacatcgcgg
     5461 tcaaggcaaa ctgtaagcgc gacagttcga ctatatctaa catcatgact ccttgctcat
     5521 cgcatgaaga ctccgagagt gaaccccgtt agaaagggtc acacgcatgc cccaatagaa
     5581 atttgtttga acactttcat cataccatgt gaatgcacac aaaaacggcg taaagaaagg
     5641 ttgcaaacat gttaataaaa actcaaattg atcccacgta tatattacgc tgcaaaatcc
     5701 ttacaataaa caggtttttg ttgaagcaaa tttgcatttt tcgacggtga tcaatttata
     5761 gctaaattac cgcctttcag ccaatttgat cgagaacaat ttatctcttt ttgatgccaa
     5821 tttccaagac ttatacattg ataaatatca attcctacaa attatgt
//
LOCUS       DAZPAC010000239         5848 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZPAC010000239 DAZPAC010000000
VERSION     DAZPAC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.240"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..461)
                     /gene="slyB"
                     /locus_tag="ACHUPM_003805"
     CDS             complement(<1..461)
                     /gene="slyB"
                     /locus_tag="ACHUPM_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR0871464.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSP"
     gene            735..1844
                     /gene="anmK"
                     /locus_tag="ACHUPM_003806"
     CDS             735..1844
                     /gene="anmK"
                     /locus_tag="ACHUPM_003806"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR0871465.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            1942..2271
                     /gene="mliC"
                     /locus_tag="ACHUPM_003807"
     CDS             1942..2271
                     /gene="mliC"
                     /locus_tag="ACHUPM_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR0871466.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            2330..2986
                     /gene="pdxH"
                     /locus_tag="ACHUPM_003808"
     CDS             2330..2986
                     /gene="pdxH"
                     /locus_tag="ACHUPM_003808"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR0871467.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            3115..4389
                     /gene="tyrS"
                     /locus_tag="ACHUPM_003809"
     CDS             3115..4389
                     /gene="tyrS"
                     /locus_tag="ACHUPM_003809"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR0871468.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            4448..5311
                     /gene="pdxY"
                     /locus_tag="ACHUPM_003810"
     CDS             4448..5311
                     /gene="pdxY"
                     /locus_tag="ACHUPM_003810"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR0871469.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(5355..>5848)
                     /gene="gstA"
                     /locus_tag="ACHUPM_003811"
     CDS             complement(5355..>5848)
                     /gene="gstA"
                     /locus_tag="ACHUPM_003811"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR0871470.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
BASE COUNT         1441 a         1459 c         1529 g         1419 t
ORIGIN      
        1 ggagaaacgg tcacctgact gccattgctg gccagtacga cacgttggcc cggagagaaa
       61 cgagtgttgc cttgtttctg taccaccatg atggtattac catcgtcttt acgaatttcc
      121 agctcgacac cctgcgtttt gttcattgca ctctgtacgc cctgaccagc tacgccacct
      181 gcaacagcgc ctgctgcagt agccagagaa cgcccggttc cgccaccaac agtattcccc
      241 aggaaaccac caagaacagc accgccaatt gcaccgataa cgttggaatc atcaccgccc
      301 tgaatctgta ccggacgtac gttaacgatg gtgccatagc tgacattctg tacttgtttc
      361 gcttcagaag cggtataaac atcccctgac agggtgtcgt tattaacaca accgacaaga
      421 gacagaccta ccattgaaac aaccaatacg cgtttaatca ttgaaaaatc tcctgttcac
      481 catgaaacgc cacgcaagca tccctcatgg tcaaagtata tggcatatgt gaatccatgg
      541 ttacatgttc taccaaatat gagcaaatca taatgaaagt aggatcaacc aattataaac
      601 aaaacattca tgatgattat tgatttgaag ataatgtttt actaccaacg gtaaaaatcg
      661 gtcattccgc atggtatcca cgaaaagttt atgtttgagt ggtgcgcaaa cgtcagattg
      721 aaaaaggatg aactatgaaa tcgggccgct ttattggcgt tatgtcaggc accagccttg
      781 atggtgttga tgttgtgttg gcgacaatag atgaacaccg ggtcgcacag ctggcaagct
      841 tgagctggcc gatcccggtg tctctgaaac aggctgtact ggatatttgc cagggccagc
      901 agcttacact ttcgcagttt ggacagcttg atactcaact cgggcgactt tttgctgatg
      961 cggtcaatgc cttgcttaag gaacaaaacc tgcaggcgag agatatagtt gcgatcggct
     1021 gtcacggtca aaccgtctgg catgaaccga cgggcgtggc accacacact ttacagattg
     1081 gcgataacaa tcaaattgtg gcacgcaccg gaattacggt tgtcggtgat tttcgccgtc
     1141 gcgatattgc cttgggagga caaggcgcac cgctggtacc tgcgttccat catgccttgc
     1201 tggctcaccc aaccgagcga cgaatggtgc tcaatattgg cggcatcgcc aatctgtcac
     1261 tgctcattcc tgggcagccg gttggcggct acgataccgg tcctggtaac atgctgatgg
     1321 atgcctggat ctggcgtcag gccggtaaac cttacgataa agatgccgag tgggcacggg
     1381 cgggtaaagt tattctccca ctgctgcaaa atatgctcag cgacccatat ttctcgcaac
     1441 ctgcaccgaa aagcaccgga cgcgaatact ttaactacgg ctggctggag cgccatttac
     1501 gccattttcc gggtgttgat ccccgtgatg ttcaggccac actggcagaa ctcaccgccg
     1561 tgaccatttc tgaacaagtt ttgttgagcg gtggctgcga acgattgatg gtatgtggtg
     1621 gaggtagtcg taacccgcta ctcatggcgc gtctggcggc attactgcca ggcacagaag
     1681 tcaccaccac cgatgccgtt ggcattagtg gcgatgacat ggaagcattg gcattcgcct
     1741 ggcttgcctg gcggacgctg gcgggattac caggaaatct gccttccgtc actggcgcaa
     1801 gccaggagac ggtactgggg gctattttcc ctgctaaccc gtgatatcag agttaactga
     1861 atttttctta ccgcgtctgc cgttacactt gaaacgttag ggagggcgta ttgccctcca
     1921 gaccaggaaa gtcttcggga aatgtctatg aaaaaactgt tactgatctg tttgccggtg
     1981 cttctcaccg gctgtagcgc gttcaatcaa ctggttgagc acatgcaaac cgctacgctg
     2041 gaataccagt gtgatgaaaa accgttgacg gtcaaactga ataatccgcg ccaggaagtc
     2101 agttttgttt acgataatca actactgcat ctcaaacagg gcatttcagc gtctggtgta
     2161 cgttacactg acggaatcta tgttttctgg tcgaaaggcg atgaagcgac tgtctataaa
     2221 cgcgaccgca tcgtattgaa taactgtcag ttacaaaatc cacagcgttg agatttttcc
     2281 aggggcggcg cacaatagcg tcacccactg acaatccgta aagaaaacca tgtctgataa
     2341 cgacgaattg cagcaaatcg cgcatctgcg ccgtgaatac accaaaggcg ggttacgccg
     2401 tcgcgatctt cccgccgatc cattaaccct ttttgaacgt tggctctctc aggcgtgtga
     2461 agccaaactg gcagacccta ccgcgatggt ggtcgctacc gtggatgaac atgctcagcc
     2521 ttatcaacgc atcgttttac tcaaacatta cgacgaaaaa ggcatggtgt tttacaccaa
     2581 cctcggcagc cgtaaagcac atcaaattga aaataatccg cgcgttagcc tgctgttccc
     2641 gtggcatacc cttgagcgcc aggtgatggt gatcggtaaa gcggaacgac tttcgactct
     2701 cgaagtgatg aaatattttc atagccgccc gcgtgatagc cagattggtg catgggtttc
     2761 gaagcagtcc agtcgcattt ctgcccgcgg tatccttgaa agtaaattcc tggagctgaa
     2821 gcagaagttt caacagggcg aagtgccatt gccgagcttt tggggcggtt ttcgcgtcag
     2881 ccttgaacag attgagttct ggcagggcgg tgagcatcgc ctgcatgacc gctttttgta
     2941 ccagcgtgaa aatgatgcgt ggaagattga tcgtcttgca ccctgaaaag atgcaaaaat
     3001 cttgctttaa tcgctggtac tcctgattct ggcactttat tctatgtctc tttcgcatct
     3061 ggcgaaaagt cgtgtaccgg caaaggtgca gtcgttatat acatggagat tttgatggca
     3121 agcagtaact tgattaaaca attgcaagag cgggggctgg tagcccaggt gacggacgag
     3181 gaagcgttag cagagcgact ggcgcaaggc ccgatcgcgc tctattgcgg cttcgatcct
     3241 accgctgaca gcttgcattt ggggcatctt gttccattgt tatgcctgaa acgcttccag
     3301 caggcgggcc acaagccggt tgcgctggta ggcggcgcga cgggtctgat tggcgacccg
     3361 agcttcaaag ctgccgagcg taagctgaac accgaagaaa ctgttcagga gtgggtggac
     3421 aaaatccgca agcaggttgc cccgttcctt gatttcgact gtggagaaaa ctctgctatc
     3481 gcggcgaaca actatgactg gttcggcaat atgaatgtgc tgaccttcct gcgcgatatt
     3541 ggcaaacact tctccgttaa ccagatgatc aacaaagaag cggttaagca gcgtctcaac
     3601 cgtgaagatc aggggatttc gttcactgag ttttcctaca acctgttgca gggttatgac
     3661 ttcgcctgtc tgaacaaaca gtacggtgtg gtgctgcaaa ttggtggttc tgaccagtgg
     3721 ggtaacatca cttctggtat cgacctgacc cgtcgtctgc atcagaatca ggtgtttggc
     3781 ctgaccgttc cgctgatcac caaagcagac ggcaccaaat ttggtaaaac tgaaggcggc
     3841 gcagtctggt tggatccgaa gaaaaccagc ccgtacaaat tctaccagtt ctggatcaac
     3901 actgcggatg ccgacgttta ccgcttcctg aagttcttca cctttatgag cattgaagag
     3961 atcaacgccc tggaagaaga agataaaaac agcggtaaag caccgcgcgc ccagtatgta
     4021 ctggcggagc aggtgactcg tctggttcac ggtgaagaag gtttgcaggc ggcaaaacgt
     4081 attaccgaat gcctgttcag cggttccttg agtgcgctga gtgaagcgga cttcgaacag
     4141 ctggcgcagg acggcgtacc gatggttgag atggaaaagg gcgcagacct gatgcaggcg
     4201 ctggtcgatt ctgaactgca accttcccgt ggtcaggcgc gtaaaactat cgcctccaat
     4261 gccatcacca ttaacggtga aaaacagtcc gatcctgaat acttctttaa agaagaagat
     4321 cgtctgtttg gtcgttttac cttactgcgt cgcggcaaaa agaattactg tctgatttgc
     4381 tggaaataat gcattaagtg gaaaggggga gtgagaaatc actccccctg gtttttatac
     4441 agggaacatg atgaaaaata ttctcgctat ccagtctcac gttgtttatg gtcatgcggg
     4501 taacagtgcg gcagagtttc cgatgcgccg cctgggcgcg aacgtctggc cgctgaacac
     4561 cgttcaattt tctaatcaca cccaatacgg caaatggact ggctgcgtga tgccgcccag
     4621 ccatttaacc gaaattgtgc aaggcattgc cgctattgat aaattacaca cctgtgatgc
     4681 cgtgttaagt ggctatctgg gatcggcgga gcagggtgaa catatcctcg gtatcgtccg
     4741 tcaggtgaaa gcagcgaatc cgcaggcgaa atatttttgc gatccggtaa tggggcatcc
     4801 ggaaaaaggc tgtatcgttg caccgggtgt cgcagagttt catgtgcggc acggtttgcc
     4861 tgccagcgat atcattgcgc cgaatctggt tgagctggaa atactctgtg agcatgcggt
     4921 aaataacgtc gaagaagcgg ttctggcagc gcgcgaactc attgcgcaag ggccacaaat
     4981 tgtgttggtt aaacacctgg cgcgagctgg ctacagccgt gaccgttttg aaatgctgct
     5041 ggtcaccgcc gatgaagcct ggcatatcag ccgtccactg gtggattttg gtatgcgcca
     5101 gccggtaggt gttggtgatg tgacgagcgg tttactgctg gtgaaactgc ttcagggggc
     5161 aacgctgcag gaggtcctgg aacatgtgac cgctgcagtc tacgaaatca tggtgaccac
     5221 caaagcaatg caggaatatg agctgcaagt ggtggctgct caggatcgta ttgccaaacc
     5281 agaacattac ttcagcgcaa caaagctctg aaatatcaca tttcggccca caacgacctg
     5341 tgggccgatg gctattactt taagccttcc gctgacagcg cgtcttgtac ttccggacgt
     5401 tcagccatac gttgcataaa tgccgcaatg tgctctaacc cttccagatt cagtttcacc
     5461 gcgtatgccc agcgcagaac cgtaaacaga taggcatcag caattgtaaa tctttgcccg
     5521 cagatccagt gctcatcctt cagtgcctcg ttcacatatt gcagcttctt atccagctgc
     5581 gcgcgaactg tcggtttgta ctcttccggt gtatctgggc gaaacagagg tgtgaaacct
     5641 ttatgcagct cggtggcgat gtaattcagc cattcgatgg ttttatagcg ggaaatactg
     5701 tttaccggtg ccagcaactg gcggtcgggg acgctgtcgg caagatactg cataatcgct
     5761 acgccttccg tcagcaaagt accgtcatcc agcagcaatg ctggcacctg ccccttaggg
     5821 ttaacggcaa agtaatcgtc accgtttt
//
LOCUS       DAZPAC010000240         5843 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZPAC010000240 DAZPAC010000000
VERSION     DAZPAC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.241"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(38..400)
                     /locus_tag="ACHUPM_003812"
     CDS             complement(38..400)
                     /locus_tag="ACHUPM_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR0871471.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="ACHUPM_003813"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="ACHUPM_003813"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR0871472.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHUPM_003814"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHUPM_003814"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0871473.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHUPM_003815"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHUPM_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR0871474.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHUPM_003816"
     CDS             complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHUPM_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR0871475.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5321..5683
                     /locus_tag="ACHUPM_003817"
     CDS             5321..5683
                     /locus_tag="ACHUPM_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR0871476.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1471 a         1445 c         1519 g         1408 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
     5341 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
     5401 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
     5461 agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct
     5521 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct
     5581 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
     5641 gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc agacgactgg
     5701 ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
     5761 ctactcgcct aattaccaaa caattcactc cctttacgtc gatttaccgc tggttgagat
     5821 tctgaaaatg catttttacc gaa
//
LOCUS       DAZPAC010000241         5836 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZPAC010000241 DAZPAC010000000
VERSION     DAZPAC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.242"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHUPM_003818"
     CDS             complement(<1..242)
                     /locus_tag="ACHUPM_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0871477.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(604..3387)
                     /locus_tag="ACHUPM_003819"
     CDS             complement(604..3387)
                     /locus_tag="ACHUPM_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0871478.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAELSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3444..5816)
                     /locus_tag="ACHUPM_003820"
     CDS             complement(3444..5816)
                     /locus_tag="ACHUPM_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0871479.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1489 a         1319 c         1311 g         1717 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatagc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaaataa caaaatccta atgttattta tcgtgagata
      361 ttacgagaat aatatttttt cattgaaaaa caatataata tgaaattctt gggtggtggt
      421 aaggtgtttt atgctgttat ttttatgcgc attctgtgcc tcctgaattt tcacgtaaaa
      481 atcagacctt aaaatatcac tattagtacc tggttattat tttgaacgca tttataaaat
      541 tattacataa aaatggcgaa tattgctaaa atccccgcca acgatgtgtt gacggggctg
      601 ttattatttt ggcaataata ctccagtata agtatttacc ggatgagaaa gatattgttt
      661 aacggcagtg ttaacattct ctaccgtcat ttgtttcaac aattgctcct gctcagtcca
      721 tgctgcagga tcgtcatatt gaataagact atttacaata gtgttcgcta attgttgaac
      781 gctacgctgt tggatatcga ggctgcgctg aacgttttgc tggtattcat tcagttcttg
      841 ctcactgatc cctttagcca gacgcttaac catcacttca ttcgctaacg ttaacagttc
      901 atcatgtcgt tctggttgac aagtaaaagc cagcaaatga ctgatatctt tggcctgagg
      961 atcaaccgag aggcgagaag aaacgctgta tgctccagat gcctgttcac gaatattaac
     1021 gcgtagatct tttgccagtg cgacgttaaa agcatcgagc gccatacgcg tcgccagatt
     1081 aacaggtgtc cgggaatcat aacgcttcca ctgtgaaacc tgtgccacag gttcattttg
     1141 ttcttttaca gtaaccgatg cgttgtccgt cgcgcgagtt aatggtttac ctgcggctaa
     1201 tggcgaatca gagtgtttga ttgatcctaa gtaacgcgta attaacgcca cgagtttgtc
     1261 ttctgagaca ttaccgacaa tgacaaacgt gatatccgct ggagatgaaa acaactggcg
     1321 atcggcagcc agcgcatctg cggcagtaaa ctgtacaatc tgattttctt gcagtaattt
     1381 cgtgcggtca tcagcatagc gcgtctcata catctgctgg gcgaatttct ccgctggacg
     1441 ctggtcgagc gttttcaacg cctgaatttg agcattttgt agcgatgccc aaatattgtc
     1501 gttaatcgtg ctgtgggtga ttcgctggtt aatcaactga aaaccaggtt cagggttatt
     1561 agtccgcgcg ctaacggaga gcaacgtatt catgccactg actttactgc tcatggttac
     1621 cgaattttcc gcactccagc gtttcaggct ggaagaggag agctcaccaa cgccgcttcc
     1681 gcttactgct ttatttgcca gcgcgataag tgatttttgc tgcgcaggga aacttaaatc
     1741 gcctttatta gatacggcag taatttgtag cttttgctct tcacccgcgg attttgccag
     1801 aataaccctg gcaccattgg aaagtgttaa tgatgtcaga ttctcagcca gcgtttcttt
     1861 gctgctaatt tccgcctgtg ggtcagcgtc tactgttaac gataaatttc tgcctgggaa
     1921 gatgtaagcc gccagctttt tgttggcgta ctctttttcc agcgccagga tagccgcaag
     1981 agacaatgct tttttggcgg caagctcatt gtttaccatt tgctcccaaa atgcgtcctg
     2041 gttctttctt aactgctgcc atttttccgc cagactttgc acggtaattt gctgccacag
     2101 acgtttcgaa agttgatatg tctcttccgg cgacaagaac ggcgtattat ttaatgagct
     2161 ggatgccagg cgactggtca gcatacgtaa atcacgttca gcttgctgat caaccgcatt
     2221 tttcagccag gtgaggcggg tagatttgac atcatcgagt tcttcagcag aaaaaccatg
     2281 ctgatcaatg gttgccaact ctgccattaa tgcatttgca gcatcctgca tattatcgtc
     2341 tcgtgcattt acacggaaaa acagcgactg ataatcgggt gcaattttaa cgctgcgcgc
     2401 agtgccgcca gaaatagtct tcaactcgcc cgactgtatg cgttcctgca gacgttgatt
     2461 gaacagctga actaacatgc tcaattcagc ttgttcgaca aagctttgct catcgttcac
     2521 ttgtaccatt ggcaggcgat aatagagtgc gatgccgttc actcggtttt ctttatcatt
     2581 aatgatatta aagcgcaggt ggttttcggc ttttgtcggc cagacgcgat tttcagctgc
     2641 tttgttagcc ggaagcttac ttaaattatc ctttatcagc gccagcgctt ctttactgtc
     2701 gatatcgccg accacgataa aggtcatatt atttggttga taccagcgtt gataaaattg
     2761 gcgcaattgt gccggtgtga ccgtggcgac agtatccatc aggccgatag gttcacggtc
     2821 taaattacgg gtatttgcca gcaggaaagg gcggcgcgcc tgagaggtgc gccatttcgc
     2881 atcctgatgg gcacgccatt cctcagtaat tacgccgcgt tcagcgtcta cttcgagtat
     2941 ttcaaaggtt gcggcattac tccattcact gaagattgcc atcacttgtt gcagattttg
     3001 tttctgcgta gtcggcagac tcacctgata cagcgtttcg tcatagctgg tataggcatt
     3061 aacatcgcga ccgaaacgca ggcccattga ctcaaatgtt tcgatgactt tattacccgg
     3121 ccatgttttt gtgccgttaa acatcatatg ttctacaaaa tgagccacgc cgcgctcatt
     3181 gtcttcttcc tgcaatgaac cggtatgaat ttgcagccat aaatttacct gatcctttgg
     3241 ttgagcatgc ggataaatca tatatcgcaa gccattgtcc agttgcccgg taattaactt
     3301 ttcatcctgc ggtaaggcgg cggcaatcag ccgcccgggg agcaacagag ttgccactaa
     3361 cgtcagtaag aaacagaggt ttctcataat tatctccatg cgaaaaccgg gcgaatttac
     3421 ccggttaagt aaaatccgaa ctattaaaat ttcatgctga catcaagcca gaaagtacgg
     3481 ccactggcgt aggtcgccac accggtgttc gttgtatcaa cagcggtttt gctatccagt
     3541 acattgagaa tatcggcact gatcgtcagg ttttgttgtt tcagaaattg tggcgtccag
     3601 gacaagcggg tgtcccaggt caggctgcta tccagttttt cgtcaacata ctgcttgtaa
     3661 tctgaatatt cgctgatgta ttgcgcattt gtcttaccaa ggataatgcg agctttacgc
     3721 gcttcttgcc aggccagcgt attcgcccac actaacccgc tcggttgatg cgtgaaatcc
     3781 atgtttaagg agatctttaa tgggttatta aaatctgcca ctggaacact atcgtaagag
     3841 accagattac cgttataaac cacctggtta tcaccggtat tgctctcctc ataaccattg
     3901 tttaacgaca agttgccctt gctcttgata tagctaaaga caatttgtgg gttaatatct
     3961 acttggcgga tatgcagggg ttcggccagt tcaaaactga ggctgaacga atgcgttttg
     4021 gttttgccat cgttgttata ttcagtaatg gtggttttag tcgcgctgtc ggtacgactg
     4081 cttttgctga tttgatcatg cgcttcacgg taaacatagt ttgcacgcgc aataacgttc
     4141 ttaccgattt tctgctgcaa tcccattgcc agttcatcgt tataaggcgt tttcaaatcc
     4201 tgataacgcg tcagagtttt attacctgat accgattccg tccagctatt gcggatatca
     4261 cgtaatccca tatcaagaat attcccgcca tagtaacggt tataaccggc ggtaatcatt
     4321 gaggtttgat cagcaaaaat atcccattcc gtcataaagc gcggggagat attgtggttt
     4381 gacagatagt tgtcatagtc ataacgcaca ccgggcatta acgacacatt ccgccagcta
     4441 atgtgctccg ccatatacag cgtgtagttg tcaattccca ggctgccttt acctttatgg
     4501 taaatggtat ggttagtttt ctttccggca gcattaatca cataggattc agactggtta
     4561 tggcgttcag tccatgcatc ggaatagatg tattccgcgc cgaagtaggg ttgatgcgaa
     4621 acatcaccca cggcgaattt ttgccagtcc aggcgtgttt tgaaggtgta attatctaca
     4681 gcctgggaaa tgtgtcctaa tccgccacag gtacaacgcc ctgtaatatc accatatgta
     4741 catgaaagtt cggtgtacca gatgtcatga tcgtgacggg tataatcact aatatgatcc
     4801 caaccaacgg tggtacgtag tttggcccat gcgagttgcg tatccatatc ccatgccaga
     4861 ccataggatt tattacccat ttcgcgatca gactgcggga aggtgctggt attataatca
     4921 cggctggagc cggtatattt taaggttaaa tcgtgggtaa agcggtcgct ggcaaaccag
     4981 gtaaatttgc tcaatgcagt atcgataacg tttttatact gtgcccgacc ggtgacaatg
     5041 ccgtcattcg aaacataatc cgcgcgggtg atatcagact ggcggcgcga taaaccggcg
     5101 gtaacgccga agttatcagc gagttcctga ttaaacgaca aggtataaaa gttcttttta
     5161 aaatctgggg agtaataagt acttcctgaa gagccttgat taaatgcgct cttgttattc
     5221 tcatcgatat gcgatgttaa ccagtccgaa cgcgtagtgc gataacccaa tttcaccttg
     5281 ctatcatcag cgttgaagcg tttgatcttt gcatcaatta ccccgccatt gaagcgacca
     5341 aattcaaccg gcacaaaact gtcataaagc gtcacattgt ccagtaagct gacatcaaga
     5401 taatacccct gtgacatccc gctaatattg gttgcactac tggcatcgga ctcattcgct
     5461 gggttcaggt tattagttgc actaataccg tcaatcaaat aggcattctg gtagggcgac
     5521 gcaccgtgaa tagagatttt ctcagggcga atatctccct ggttcaacga ggtactttgc
     5581 gttgaatcca tgcgtaccgc agggttggtt ctcagcagat cgctgatatt gccattcccg
     5641 gtcggtaaac gctcgatgct ttcactggtg taatgcgtgt tgccgtttac gggtacaggg
     5701 acaggagcat agaccgtcat ctcttcaaaa aaatgcatgt acatgttttt gtcatcgacg
     5761 gcctgcgcca catcagcgcc acataaaatg acgccaggaa taagaactcg cttcatttat
     5821 ttatatctcc gtacag
//
LOCUS       DAZPAC010000242         5828 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZPAC010000242 DAZPAC010000000
VERSION     DAZPAC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.243"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..188
                     /gene="adk"
                     /locus_tag="ACHUPM_003821"
     CDS             <1..188
                     /gene="adk"
                     /locus_tag="ACHUPM_003821"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="essential enzyme that recycles AMP in active cells;
                     converts ATP and AMP to two molecules of ADP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR0871480.1"
                     /translation="TTRKDDQEETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVD
                     GTKSVAEVRADLEKILG"
     gene            320..1282
                     /gene="hemH"
                     /locus_tag="ACHUPM_003822"
     CDS             320..1282
                     /gene="hemH"
                     /locus_tag="ACHUPM_003822"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR0871481.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(1279..2238)
                     /gene="aes"
                     /locus_tag="ACHUPM_003823"
     CDS             complement(1279..2238)
                     /gene="aes"
                     /locus_tag="ACHUPM_003823"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFR0871482.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            2390..3694
                     /gene="gsk"
                     /locus_tag="ACHUPM_003824"
     CDS             2390..3694
                     /gene="gsk"
                     /locus_tag="ACHUPM_003824"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR0871483.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(3827..5503)
                     /gene="ybaL"
                     /locus_tag="ACHUPM_003825"
     CDS             complement(3827..5503)
                     /gene="ybaL"
                     /locus_tag="ACHUPM_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR0871484.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(5741..>5828)
                     /locus_tag="ACHUPM_003826"
     CDS             complement(5741..>5828)
                     /locus_tag="ACHUPM_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871485.1"
                     /translation="IELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         1495 a         1574 c         1475 g         1284 t
ORIGIN      
        1 tgactacccg taaagacgat caggaagaaa ccgtgcgtaa acgtctggtt gaataccatc
       61 agatgactgc accgctgatc ggctactact tcaaagaagc ggaagcgggt aacaccaaat
      121 acgcgaaagt tgacggcacc aagtcggttg ctgaagttcg cgctgatctg gaaaaaatcc
      181 tcggctaatc aagccgcagc agatctgctt tcgggcaggt ctgctcatcc acctcaggca
      241 atacctctca cagcaattag ttcttcttcc tcacttttcc gctacaatta tcaacaagtt
      301 gaatcgataa gaggcggtaa tgcgtcagac taaaatcggt atcctgctgg caaacctggg
      361 tacgcccgat gcccccacac ctgaagcggt aaaacgctat ctgaaacaat ttttaagcga
      421 cagacgcgtg gttgatacct cacggttgtt atggtggcca ttgctgcgcg gcgttatttt
      481 gccgctgcgc tcgccgcgtg tggcgaagct gtacgcctcg gtctggatgg aaggtggctc
      541 gccgctgatg gtttacagcc gccagcaaca gcaggcgctg gcacaacgtt taccggagac
      601 gcccgtagcg ctgggaatga gctacggctc gccatcactg gaaagcgccg tcgatgaact
      661 cctggcagag catgtagatc atattgtggt gctgccgctt tatccgcaat tctcctgttc
      721 tacggtcggt gcggtatggg atgaactggc acgcattctg gcgcgcaaac gtagcattcc
      781 ggggatatcg tttattcgtg attacgccga taaccacgat tacattaatg cactggcgaa
      841 cagcgtacgc gcttcttttg ccaaacatgg cgaaccggat ctgctgctgc tctcttatca
      901 tggcattccc cagcgttatg cagatgaagg cgatgattac ccgcaacgtt gccgcacaac
      961 gactcgcgaa ctggcttccg cactggggat ggcaccggaa aaagtgatga tgacctttca
     1021 gtcgcgcttt ggccgggaac cctggctgat gccttatacc gacgaaacgc tgaaaatgct
     1081 cggagaaaaa ggcgtaggtc atatacaggt gatgtgcccg ggctttgctg cggattgtct
     1141 ggagacgctg gaagagattg ccgagcaaaa ccgtgaggtc ttcctcggtg cgggcgggaa
     1201 aaaatatgaa tatattccag cgcttaatgc cacgccggaa catatcgaaa tgatggctaa
     1261 tcttgttgcc gcgtatcgct aaagctgagc ggtaaagaac tgagcgccgt cgcgaagagc
     1321 ctcgtcggcg gttttcatca tccgtgaata atgcaaaaag gcatgcagcg tacctgggta
     1381 gagtttgaac tcacagggct gctgatgcgc cgctaacgtc tggtaaagca gacggctgtc
     1441 atccagcagc ggatcgaact ccgccccggc aataaaacag ggcggaactt cgcgagtgag
     1501 atcattatta aacagacagt aatacggtga ctcgcggtcc gcgtcgttgc ttaaatacgc
     1561 ctcttcatac atctgcaaat cctgttgcgt taagccatcc cagacaccgc ccaacagacg
     1621 acgagtcacg gaatcccgta atccgtaaag cccataccac agcaaaacac ccgcaatttt
     1681 accgcaatcg atctgtttat cacgcaacca caacgcactg gcgagcgcca gcatggcacc
     1741 tgcggaatca ccggcaaagc caatgcggga catattgatt tgataatcct ccgcctgctg
     1801 gtggaaataa caacaagcag ccacaatttc ctctatcgct tgcggaaaac gcgcttcagg
     1861 tgaaagggtg taatcaatac caatcaccgt acattggctg tagcttgcca gcaggcgcat
     1921 gatgcgatcg tgggtatcga gattgccgag aataaaaccg cctccatgca aataaaatag
     1981 cgtcgccggg ctatctggct gcggacaaaa gagacgtgtt tccacctgcc catattttgt
     2041 tggaaccatg taagctctgg ttgccatttc tggagcgccc gcattccaga atcggcgctc
     2101 aagcgtgtaa tactgtcgtt gctcagcaat cgttcccgtt gcgggccagg acggtaaatc
     2161 cggctgaaga gtattcacaa cggtcttcat ttcagcagaa ataaggtcca gaacaggtag
     2221 tttgttttcc ggcttcatac aaaaactcct ttcaaattac gtcattgtaa ggaaccactg
     2281 ccatgaaaat gcgatcccgc ctgctgatat tgaaactggc tgcgtctcgc gcgctcccgt
     2341 cagattgtgt taacattcgc cgctcagtta accacccgta aaaacaacca tgaaatttcc
     2401 cggtaaacgt aaatccaaac attacttccc tgtaaatgca cgcgatccgc tgcttcagca
     2461 attccagcca gaaaacgaaa ccagcgccgc ctgggtcgta ggtattgacc agacgctggt
     2521 cgatattgaa gcgaaagtgg atgatgaatt cattgagcgt tatggattaa gcgccgggca
     2581 ttcactggtg attgaggatg acgtagccga agcgctttat caggagctaa aacagaaaaa
     2641 cctgattacc catcagtttg cgggtggcac cattggtaac accatgcaca actactcggt
     2701 gctcgcggac gaccgttcgg tgctgctggg cgtcatgtgc agcaatattg aaattggcag
     2761 ttatgcctat cgttacctgt gtaacacttc cagccgtacc gatcttaact atctacaagg
     2821 cgtggatggc ccgattggtc gttgctttac gctgattggc gagtccgggg aacgtacctt
     2881 tgctatcagt ccaggccaca tgaaccagct gcgggctgaa agcattccgg aagatgtgat
     2941 tgccggagcc tcggcactgg ttctcacctc ttatctggtg cgttgcaaac cgggtgaacc
     3001 catgccggaa gcaaccatga aagccattga gtacgcgaag aaatataacg taccggtggt
     3061 gctgacgctg ggcaccaagt ttgtcattgc cgagaatccg cagtggtggc agcaattcct
     3121 caaagatcac gtctctatcc ttgcgatgaa cgaagatgaa gccgaagcgt tgaccggaga
     3181 aagcgatccg ttgttggcat ctgacaaggc gctggactgg gtagatctgg tgctgtgcac
     3241 cgccgggcca atcggcttgt atatggcggg ctttaccgaa gacgaagcga aacgtaaaac
     3301 ccagcatccg ctgctgccgg gcgctatagc ggaattcaac cagtatgagt ttagccgcgc
     3361 catgcgccac aaggattgcc agaatccgct gcgtgtctat tcgcacattg cgccgtacat
     3421 gggcgggccg gaaaaaatca tgaacactaa tggagcgggg gatggcgcat tggcagcgtt
     3481 gctgcatgac attaccgcca acagctacca tcgtagcaac gtaccaaact ccagcaaaca
     3541 taaattcacc tggttaactt attcatcgtt agcgcaggtg tgtaaatatg ctaaccgtgt
     3601 gagctatcag gtactgaacc agcattcacc tcgtttaacg cgcggcttgc cggagcgtga
     3661 agacagcctg gaagagtctt actgggatcg ttaagttatc gtcggttcgt aggccagata
     3721 aggcgttcac gccgcatctg gcatttggct ctcgatgcct gatgcgacgc tggcgcgtct
     3781 tatcatgcct acatattttt catattttac atccggcaac caccgtttac cccgtcacca
     3841 cttcacccgc cggtggcgtt tccagcagtt ccagcatagt acgggcaatt tcacgttcac
     3901 ccatcactac ctgattcgcg ccacgttcgg tgatatacgt cacttcatcg tcataatggg
     3961 cgcgggcgat aatctcaata tccgggtttt tcgcgcgggc agacgccaca atctcacccg
     4021 cttcataacc gttggggatc gtcaggatca gccattttgc acattccaga tgcgccagtt
     4081 gcataatttc ttcgttcgct gcattgccca atacagcgcg gaccccacgc tctcgcagct
     4141 catcaacacg ggttcgtgac gtctcaatca ccaccagcgg aatatcagag gcgagcaatt
     4201 tctcccccag caggctgcct acacgaccgt aacccaccaa tagcgcatgg ttgcaaatat
     4261 ccactgggat ctgtttctct tcttcgattg cctcttccag cgtctgctct tccagcgttt
     4321 cggtcttcgc cagatatttc tccagtagtg cgaacagtac cgggttgagc ataatcgaca
     4381 ggatcgcccc tgccagtacc aggttttgtc cggcctgcgg cagtaaattc aatgccattc
     4441 ccagtcccgc caggataaac gcgaactcac caatctgcgc caggctggcg gcgatggtta
     4501 atgccgtacg ttgggagtga ccaaacagac ggacaaggaa cagggccgcg accgatttac
     4561 caaacagaat aattgccagc gtcgccagca ctgccagcgg ttgctgaatc agaattaacg
     4621 gatcaaacaa catcccgacg gagacaaaaa acagcaccgc aaacgcgtcg cgcaatggca
     4681 gcgtatcgtg ggcggcacgg tgactcagtt cagactcgtt cagtaccatc ccggcaaaga
     4741 acgcgccaag tgcgaaggag acgtcaaaca actccaccgc accaaaggca atccctaacg
     4801 ccagcgccag caccgacagg gtaaatagct cgcgagaacc ggttgccgcg ctgcgtgcca
     4861 taatccacgg caccagacgg cgacctacca gcatcataat ggcgataaat gcgatcactt
     4921 tgccgatggt gatccccata tcgactgcaa gagtggcaaa gcccacatcg ccctgttcca
     4981 tcattcctgc cactgcgggc agcaacacca gcgtcagaac cattaccagg tcttccacaa
     5041 tcaaccaacc gatggcgatt tgcccacgct gactgtcaat taattgccgt tcttcaagtg
     5101 cgcgcagtaa caccacggta ctggcggtgg aaagacataa accgaacacg ataccggtca
     5161 ttaacgacca gcccagcacg gcagagagcg ccatacccag cagcgtcgcc acggctatct
     5221 gggcgatcgc accgggaatg gcgatggcct ttaccgccat caaatccttc agcgaaaagt
     5281 gcaaaccgac gccaaacatc aacagaatga cgcccagttc agccagttcc ggcgcaagct
     5341 tggtatcggc aacaaagccc ggagtgaatg gtcctgccag cacacccgct aacagatatc
     5401 ccaccagagg agaaatacgt agtttattgg ccagcatgcc gaggataaag gcgagcacaa
     5461 ggccgccaac aatggtggtg ataagcgggg tggcgtgatg cattccgtct ccttttcctg
     5521 gtggttattg tccatttttg gccgggaaaa ccaaaattac aggtaatagt ttatgacaat
     5581 ttcattgatg atgttcatga ataattattg aattttgcag aaaaatggaa ttagctgcaa
     5641 aaaaagcacg gatcggaaaa cagaaggcgg tttaacagag gaaaggctta tggcgactgt
     5701 aaaacgatgc agccaaagtt tgcctttggc tgcaatgaaa tcagtcttta tgccggttat
     5761 caggcaggaa tatggtcaac atccccaata gtggcaggaa agcacagatt ttatagacta
     5821 actcgatg
//
LOCUS       DAZPAC010000243         5815 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZPAC010000243 DAZPAC010000000
VERSION     DAZPAC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.244"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1239
                     /locus_tag="ACHUPM_003827"
     CDS             <1..1239
                     /locus_tag="ACHUPM_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HFR0871486.1"
                     /translation="FLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVT
                     ETSFFPQREQDARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQL
                     GFIPEQIRLLNIMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPL
                     EGQILGATQTPIICDKSDEDAVKLAWSRAISSAMRLDPDAIMEGEMRDLISMMSTTYA
                     AQTGHIVLTTLHTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIP
                     WETRAPELRDDERDYLERHCNKDSLCSTDNIWFRNPHGCSECNHDVIINGRKRGEIGK
                     GLTGRTVIAEVIEPDNRLFQILKTRGKVAARKYWLENMKGISRVEHLLRRINEGLVDP
                     LEADRIIPLDEDERLSIDDV"
     gene            1252..2349
                     /locus_tag="ACHUPM_003828"
     CDS             1252..2349
                     /locus_tag="ACHUPM_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFR0871487.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLSLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKEQIHQAIWQMAIYPVGLLIMMTGTLYVLNTQLIPELSKI
                     SSPDSWSGALGFLYGLSVFVDNYGAICAVLFAVITGLISWSLPNWKSPDSVRTFADKI
                     MPWSIYQDIQGATFLLNMAALLKAKMTTLNSLNILQEFASPWLSTRLDSIIYRVRQGD
                     HLGLALRQCGYQFPSREAANFLSLLQGDGATELISNYGQRWLSQTLQRVKKRANVIRL
                     IMLIFLVMSLMLLVFAIMDIQSISDNSMGNF"
     gene            2367..2981
                     /locus_tag="ACHUPM_003829"
     CDS             2367..2981
                     /locus_tag="ACHUPM_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFR0871488.1"
                     /translation="MLVENINATLTGNNKKNEPHDKGWGILEQGTIALVVLFVIVVVL
                     GSLYALRTRTNVATETANIQTIITSAQSLLKGSDGYTFTSSAKMTGALIQMGAVPSGM
                     TVQGDKTSGTATLYNAWGGAVTVAPASTSGFNNGFTVTYDKVPQDACIQIATRISKTG
                     LTNGITLNSTAHSDGKVTTEEASTQCKADNGSTGTNKLIFTING"
     gene            2991..3551
                     /locus_tag="ACHUPM_003830"
     CDS             2991..3551
                     /locus_tag="ACHUPM_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR0871489.1"
                     /translation="MSGGTVPLRLISLILLFSLIPPARASYVAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLRPGAININKDKKTGKASSTDYGLMQINSTHIPKLIN
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKIWRIYRDMKGICLPGQGGRQCRQS"
     gene            3536..4192
                     /locus_tag="ACHUPM_003831"
     CDS             3536..4192
                     /locus_tag="ACHUPM_003831"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR0871490.1"
                     /translation="MQTIVGISLSPVYILPLMFTFSIIGRTLLFRVRYFLSDTGHLWY
                     KTHPAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFT
                     RRFLIAGMLSQITTDIWWFRTTEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWCVLLGTGGFVLWHSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI
                     "
     gene            4192..5484
                     /locus_tag="ACHUPM_003832"
     CDS             4192..5484
                     /locus_tag="ACHUPM_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFR0871491.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEA
                     RLVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGAILSCQSGTWGTIGGKLKVTQLSTTGYLGQFDFCAIARM
                     GNAEDAHYCQVVESPAGSRKWYKYEHKTGCIASCVTLN"
     gene            complement(5481..>5815)
                     /locus_tag="ACHUPM_003833"
     CDS             complement(5481..>5815)
                     /locus_tag="ACHUPM_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin, shufflon protein A"
                     /protein_id="HFR0871492.1"
                     /translation="TSGSLNGSYTNLGSHRGSFSGRNSGGSTLFIYASGGNGGSAGGA
                     CANTSRLQGYVGGTLISVNASNNPAYGKTAFISFAVPAGTSYQITSYPTENTSCGAGV
                     FSVFGYQT"
BASE COUNT         1650 a         1285 c         1373 g         1507 t
ORIGIN      
        1 tttttaattt ctgagtctat tttcaaagtt cgcatgagaa tattcggtga actgcagact
       61 gtagatgaag atcaaccagc actgggctat tcactttgcg caacagccat tctgtcaatg
      121 gctgatgtca ctgaaaccag tttcttccct caaagggaac aggatgcgcg cctgtcccca
      181 caattaatgc gaaaaatagg aatatttggt gcacgctaca gtcacagacc tacgggagac
      241 ggtctgattg cagttatgcg cctgataccc gatgacggag ataaagttcc cacgtttaaa
      301 caactgggct ttatcccgga acaaatccgg ttgctgaaca taatgctgcg ccgcccggaa
      361 ggaaaaatag tcctgtcagg tccaaccggt tccggtaaat cgacaacgtt acgcagcgcc
      421 tgccgtgtat atcttgacga taatcagggg cgacacctgc taacaatcga agatccgctg
      481 gaaggacaga tactgggagc aacacagact ccgattatct gcgacaaatc cgacgaagat
      541 gccgtcaaac tggcatggag tcgggctatt tcatcggcaa tgcgacttga ccctgatgcc
      601 atcatggaag gggaaatgcg cgatcttatc tcgatgatgt caaccaccta cgcggcacag
      661 accgggcaca tagttctgac gacattacat acaaactcag ctctgggtat accggagcga
      721 atgattacga tgggtatgaa tgccgatctt atttgcgatg cgcaattact gattgggatg
      781 atcagccagc gccttgtacc aactctgtgt ccctcctgcc gtattccatg ggaaacgcga
      841 gcacctgaac ttagagacga cgagcgtgat taccttgagc ggcactgcaa taaagattca
      901 ctctgcagta cagataatat ctggttccga aatcctcacg gatgctctga atgtaatcac
      961 gacgtaatca ttaacggcag aaagcgcggt gaaataggta aaggcctcac cggcaggact
     1021 gtaattgctg aggtgataga acctgataac cgcttatttc agattctgaa aacacgcggc
     1081 aaagttgcag cacgtaaata ctggctggaa aacatgaaag gcatcagtcg tgtagagcat
     1141 cttcttcgcc gtatcaatga aggtctggta gaccctcttg aagccgacag aatcatccct
     1201 ctggacgaag acgagcgcct cagcatcgat gatgtctaac aggagaacaa catgcgtgaa
     1261 atgaatttca gtcaacggct gaggcgtttc attgtcagaa aaacgttcag cgccccctac
     1321 agggtgcagt tttacgaagc cctgcgtttt ctgctggaaa ataaacagcc attaaagacc
     1381 gcactggaac agatgcggga tgcctggaca gactttggac gaaaatggca tccctttgct
     1441 gaactggcca cagactgcat tgagtctctt cgtgaaaaca gtggcgaaaa ctctctggaa
     1501 tatactctta gtttatgggt tcctcaggaa gaggccgcag taatcagcgc cgggataaga
     1561 agtggttcaa ttgtggatgc actgcaattc gccaccactc ttacagatgc aaaagaacaa
     1621 atccatcagg ctatatggca aatggctatt tatccggtgg gattgttaat catgatgacg
     1681 gggacattat atgttctgaa tacacagcta atacctgaac tgagcaaaat aagctccccc
     1741 gattcatgga gtggtgctct tggttttctt tatggattat ctgtttttgt cgataattac
     1801 ggcgctatat gtgccgttct ttttgcagtg atcacaggtt taatatcatg gtctctgcca
     1861 aactggaaaa gccctgacag tgttcgtact tttgccgaca agataatgcc atggagtatt
     1921 tatcaggata tacagggagc aacatttctc ctgaatatgg ctgcacttct gaaagccaaa
     1981 atgacgacgc tcaactcact aaacattctt caggagtttg catcaccatg gttaagcaca
     2041 cgtcttgaca gcattattta ccgcgtccgc cagggggatc atcttggact ggctttgcgt
     2101 cagtgcggtt atcagttccc atccagggaa gcagccaact tcctgtcttt attacaggga
     2161 gatggcgcaa cagagctaat cagcaattat ggtcaacgat ggcttagtca aacacttcaa
     2221 cgtgtaaaga aacgggcaaa cgtgattcgt ttaattatgc tgatatttct tgtcatgtct
     2281 ctcatgttac tggtttttgc cattatggat attcagtcta ttagtgacaa cagcatgggt
     2341 aacttttaaa caaaacgagg atatttatgt tagtagaaaa tattaatgcc actttaactg
     2401 gaaataataa gaagaatgag cctcatgata aaggatgggg aattttggag cagggaacaa
     2461 tagcgctcgt tgttcttttt gttatagtcg ttgttctggg tagtttgtat gcgttacgta
     2521 caagaaccaa tgtggcaact gaaaccgcaa atatacaaac aattatcaca agtgcccaga
     2581 gcctgctaaa aggtagcgat ggatatacat ttaccagcag tgccaaaatg acaggtgccc
     2641 tcattcagat gggggccgtt ccctctggga tgacagtaca gggcgacaaa acatctggta
     2701 cagcaacgct ctataacgca tggggcggtg ctgtaactgt tgccccggct tcaacatcag
     2761 ggttcaacaa cggttttacc gttacctatg ataaagtccc acaggatgcc tgtatccaga
     2821 ttgccacacg gatcagtaaa actggtttaa ctaacggcat taccctgaac agtactgctc
     2881 acagtgatgg aaaagtcact actgaagaag ccagtacgca atgcaaggca gataatggca
     2941 gcactggtac aaacaaactt atcttcacca tcaatggtta aggagtttta atgtcagggg
     3001 gtacagtacc cctcagactt atttcattaa tcctgctttt ttctcttatt ccacccgccc
     3061 gagcttcata cgtagccccc aaacagatct ggaccaacaa gtgggacaat tgctttgccg
     3121 ctgccggcgc gcgctatcaa attgaaccat tattattgaa ggctatttct gccggggaat
     3181 cttcgttgag gccgggcgca attaatataa ataaggacaa aaaaacaggt aaagccagta
     3241 gcacggatta tgggttgatg caaataaact caacccatat tccaaagctg ataaatatgg
     3301 gcgtaataaa aaagtctgag gatttaatca ccaaaccatg ccttaatatc catataggaa
     3361 gctggatact ggcaagacat tttcagatat gtggcgtcag ctggaattgc cttgggtcat
     3421 acaatgccgg attccgtaag gacagacatg aaacaagaga gcaatatgcc aataaaatat
     3481 ggagaattta tcgtgacatg aaagggatat gtcttcccgg acagggaggc aggcaatgca
     3541 gacaatcgta ggcataagtc tatcccctgt ttatatactg ccattgatgt tcacattttc
     3601 cattattgga cgaactctgc tattcagagt tcgttatttt ttatctgata cgggacatct
     3661 ctggtataag acacaccctg ctgttctgtc aggaatatgg ctatacagta tagctgtggc
     3721 gttaattatt ttatcatcat caccactgct ctaccgtatt catgctgtcc ttattctttc
     3781 gttcatactg caaatggcgg taacggatgc actgacaggt ttattaccag gaacattcac
     3841 ccggcgtttt ttaattgcag gaatgctatc acaaatcacg actgatatat ggtggttccg
     3901 tacaacagaa tttgccactg ctgcaattgt cttattctgc cttcataaat tagttaaccg
     3961 ccatcgtctt aatattggca ctggagacct gtggcttatt gcaggtatta ccgcatggtc
     4021 agggttatat aacgccatat ggtgtgtgtt gctgggcaca ggaggatttg tcttatggca
     4081 ttcaacatgg tgcataaaag ggcataaaga aggccctctg ggtccatggc tatgttttgg
     4141 ccatgtcctt ttgctgctgg ataatcttta tcaaccacta tgggtaatct gatgaaaaaa
     4201 tatgaccgag gctgggcatc gctggaaact ggcgcggctt tactgattgt tatgctcctg
     4261 attgcctggg gagcgggcat atggcaggac tatattcaaa caaaaggatg gcaaactgaa
     4321 gcacgtcttg tcagcaactg gaccagtgcg gcccgctctt atatagggaa aaactacacg
     4381 actctccagg gcagtagtac cacaacaact cctgccgtta tcacgacaac catgctgaaa
     4441 aataccggct ttttgtccag cgggtttact gagacaaaca gcgaggggca gcggttacag
     4501 gcatatgtgg ttcgaaacgc ccaaaacccg gaattactac aggcaatggt tgtatccagt
     4561 ggtggcacac cttatccagt gaaagcactt atccagatgg ctaaggatat taccactggt
     4621 cttggtggat atatccagga cggcaaaaca gccacaggtg cattacgttc ctggtcagta
     4681 gctttaagta attatggtgc caaaagcggt aacgggcata ttgccgtatt gttatcgaca
     4741 gatgaactta gtggtgcagc tgaggacact gatcgtcttt acagattcca ggtcaatggt
     4801 cgccctgact taaacaaaat gcacacggcc attgatatgg gatcaaataa cctgaataac
     4861 gttggggcag taaatgccca gacaggtaat ttcagcggca atgtgaatgg tgtaaatggc
     4921 actttcagcg gtcaggtaaa aggcaatagc ggaaactttg acgtaaatgt caccgctggc
     4981 ggtgatatca gaagtaataa tggttggtta attactcgta acagtaaagg ctggctcaat
     5041 gaaactcacg gtggcggatt ttatatgtcc gatggatcat gggttcgaag tgtaaacaac
     5101 aagggcatct ataccggcgg tcaggtgaaa ggcggtactg ttcgggctga tggtcgcctt
     5161 tatactggtg aatacttaca actggaaaga actgccgttg ctggcgcatc atgttcgcct
     5221 aacggccttg taggccgcga taatacaggg gcaatacttt cgtgccaatc cggtacgtgg
     5281 gggacaatag gtggaaaact caaagttact cagctttcca ccacaggtta tctggggcaa
     5341 ttcgacttct gtgccattgc cagaatgggc aacgcagagg atgcccacta ctgccaggta
     5401 gttgagagcc cagcaggttc acgcaaatgg tacaaatacg agcataagac aggttgtatt
     5461 gcgtcgtgtg taacgctcaa ttaagtttga tatccaaata ctgaaaatac cccggcacca
     5521 catgatgtat tttctgttgg ataggatgtt atctgatagg aagtacctgc aggtacagca
     5581 aagctgataa aggctgtttt tccatatgca gggttattgc tggcattcac gctaattagc
     5641 gtcccaccaa catatccctg cagtcgggat gtatttgcac atgcacctcc ggcagatcct
     5701 ccattacctc cagatgcata aataaacaat gtactgcccc ctgaattccg ccctgagaat
     5761 gaacctctat gtgaacctaa gtttgtgtaa gaaccattga gcgaaccaga agtct
//
LOCUS       DAZPAC010000244         5716 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZPAC010000244 DAZPAC010000000
VERSION     DAZPAC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.245"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..297
                     /locus_tag="ACHUPM_003834"
     CDS             <1..297
                     /locus_tag="ACHUPM_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR0871493.1"
                     /translation="KKNKQRKEQKPFLIPLLNPKAYLFFAALIPTFIDNNTNITLNFF
                     ILGVLFIFISFLTDLIYIAISLTIRDKLTPSFSRYISICSSIFILGTGIYFIFT"
     gene            complement(355..1071)
                     /locus_tag="ACHUPM_003835"
     CDS             complement(355..1071)
                     /locus_tag="ACHUPM_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871494.1"
                     /translation="MQLDEHSRKIAALVAYNDKQIKEMNRLITLLNERVNDLNTFTST
                     LEREGKVVSGISYSFRDSVSNVKFYINKGMDEASCKLQKSVSDTLTSLLREKLSFTLN
                     DVKKEWVDFEEQTSKEIKESLKQTTREAEVATGIIRKTMEKYTVTRIVEKVIVLIVLF
                     LLLMIAPVFYAEYLKMNISSLMEEQKKHESMLKEWKEKAPLAEFSKCGDYRKACVLVD
                     DESGYYFNEQSPNKKYAILK"
     gene            complement(1092..2177)
                     /locus_tag="ACHUPM_003836"
     CDS             complement(1092..2177)
                     /locus_tag="ACHUPM_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR0871495.1"
                     /translation="MASYHCTVKTGAKGVALKHADYISRSGEYKNYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ARFGETPQERKSALVALRSHWADVQNEHLARHGHQSRVDHRSLKEQGIKRTPEVHLGP
                     VRVAVMNADQVCAILARRNAEREKLEAEQERDSIIDLKTTLKEALAERDNIPPSLDEL
                     GAELKAFVESSFDELNEVMQLNSLINGALDSMMKQFQVEEERKLQQERLIEAEKQRDL
                     ERERERVRQRAYSSPAPSRSRDYGMSR"
     gene            2349..2795
                     /locus_tag="ACHUPM_003837"
     CDS             2349..2795
                     /locus_tag="ACHUPM_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR0871496.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPRNHPKWQEWEKRGKELLTKDSA"
     gene            complement(3002..3931)
                     /locus_tag="ACHUPM_003838"
     CDS             complement(3002..3931)
                     /locus_tag="ACHUPM_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0871497.1"
                     /translation="MSAVLQLFREKLPHKPYCTNDFTYGVRILPKDIAILARFIQQNQ
                     PHAQHWLAFDVDRTGAAIDWSDRNCPAPNITVKNPRNGHAHLLYALTLPVRTAPDASA
                     SALKYAAVIERALCEKLGADVNYSGLICKNPFHQEWQVVEWRWELYTLDELADYLDLS
                     ASARRRVDKDYGMGRNWYLFEKGRKWSYRAIRQGWPAFSPWLDAVIQRVEMYNASLPV
                     PLSPAECRAIGKSIAKYTYRKFSPEGFSAVQAARGRKGGNANSSENQAIKGQKSKRVA
                     VATSARTLKPWVTLGISRATYYRKLKCDPDLAK"
     gene            4484..5434
                     /locus_tag="ACHUPM_003839"
     CDS             4484..5434
                     /locus_tag="ACHUPM_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871498.1"
                     /translation="MNNFINECISAPRINGMAEFFSSTASGEKHKPFRLVEIDDRLFH
                     SYDYIKFAKHLTKNSGPFVNHYFCSIPYSKEEECRLGTTVIKYAITLNKKLNTWCLGM
                     AEGAMARTISELSKGNIQTLTTSPTKENEHAFFKYGSSENAYFICTPFFLLEERLKNS
                     NLKQFNQGFDIIIEDTTFQMYSPNRSGQINHVKRLLNNDGIFIFTEKHSSEHDEYQKR
                     EIQKDYSFKQRYFSKDEINTKKEIVLNTMNLNEVSVDDMLSAIKQHFKYASIYWNSGN
                     FYSIAASNSLKHISTFINLLPKAAIPMEYVYEKLPRNISW"
     gene            5441..>5716
                     /locus_tag="ACHUPM_003840"
     CDS             5441..>5716
                     /locus_tag="ACHUPM_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR0871499.1"
                     /translation="MEYSTLLSFAIVTLSQTISIGPGVALVINNAFSHGLKSSIKTSI
                     YIRIGETIVMAISLFALSSTSSTEQHFHIIKIFGGGYLIYIGLMGLIN"
BASE COUNT         1713 a         1313 c         1108 g         1582 t
ORIGIN      
        1 aaaaaaaaca aacaaagaaa agagcaaaaa ccttttttga taccattatt aaaccccaag
       61 gcgtatcttt tctttgctgc gttaatacca acctttatag ataataatac aaatatcaca
      121 ttaaactttt ttatattggg tgttttattt atatttatat cattcctaac agatcttatt
      181 tacatagcta ttagcttaac gataagggat aaattgacgc catcattctc aagatacata
      241 agcatatgta gttccatttt tatattaggt acaggtattt attttatttt tacgtgaaac
      301 gccgttttta tactcatgca gccttagtca taagacggca tgagttactt aacactattt
      361 cagtatggca tattttttat ttggtgattg ttcattaaaa taatatccgc tttcgtcatc
      421 aactaacacg catgctttac gataatctcc acacttcgag aactcggcca atggagcttt
      481 ttctttccat tcttttaaca tggattcatg ttttttctgt tcttccatta gcgaactaat
      541 attcattttg agatattcag catagaaaac aggagcgatc attagcaata aaaataatac
      601 aatcaacaca attacctttt cgacaatcct tgttactgta tacttctcca ttgtttttct
      661 aattatacct gtcgcaactt cggcctctct ggtcgtctgc tttaatgatt cctttatctc
      721 tttacttgtt tgctcctcga aatcaaccca ctctttcttt acatcattga gagtgaaaga
      781 taatttttcg cggagtaatg acgttagtgt atctgagact gatttctgta atttacaaga
      841 tgcctcatcc attcctttat taatataaaa tttaacatta gacacactat ccctaaagga
      901 ataagaaatt ccagaaacaa ctttaccttc acgttcaaga gtacttgtaa aggtatttaa
      961 gtcattcact ctttcattaa gcagagtaat tagcctgttc atttctttta tttgtttatc
     1021 attatacgca acaagagcag caatttttct tgaatgttca tccaattgca taaaataaaa
     1081 acctccagtg cttaacggct cataccataa tcacgtgaac gtgatggtgc tggtgaggaa
     1141 tacgcacgct ggcgcactct ttctctttcc ctctccaggt ctctttgctt ttcggcttca
     1201 attagtcgtt cctgttgcaa cttccgctcc tcttccacct ggaattgctt catcattgag
     1261 tccagcgcac cgttaatcaa tgaattaagc tgcattacct cattgagttc atcaaacgag
     1321 ctttcaacaa acgccttcaa ttctgcccca agctcatcca ggcttggtgg tatgttatcc
     1381 cgttcagcta acgcttcctt caaggtcgtt ttaaggtcaa tgatgctgtc ccgttcctgc
     1441 tctgcttcta gcttctcccg ttccgcgttt ctccgtgcca gaattgcaca aacctgatca
     1501 gcattcataa cagcaacacg aacgggacca agatgaactt ccggtgtgcg cttgataccc
     1561 tgttctttca gtgaacgatg atcaacgcgc gactgatggc cgtgccgcgc aaggtgttca
     1621 ttctgcacat cagcccagtg agaacgtagc gcaaccaagg ctgattttcg ctcttgtggt
     1681 gtctcaccaa agcgtgcctt ttttgcacct ccccgctccg gtgatttgct gttggcacgt
     1741 ttaaaaaact gctgcggatc gcgttcaata ccatcattaa tccgctcgca aaacatgata
     1801 tgtgcgtgtg gttgttcctt tccgtcgatt gcacctggcg gattgtggat cgcgtactga
     1861 taggcatggc gatcaccaag ctccttttgc acgaaatctt caacaagttc tatccgttgt
     1921 tcgcgtgtca gttctcgcgg taatgcgatc tcaatttcac ggtatgccgt cccgtttttt
     1981 cgttcaaatt catctgctgc tttccatagc tctgcgggat ttttcttcgc ccatgatggc
     2041 atgttaccgg atgacgaaaa ttccagatcc tcccgcgatt tgtagttctt atattcgccg
     2101 gatctagaaa tataatcagc atgttttaag gctacgcctt ttgctccagt tttaactgta
     2161 caatggtagc ttgccatctg ccgttaagcc ctccgcagga gcgcactgtt tctcgacgaa
     2221 ggagagaaac gtctaagtgc gcatccctga attaatcagg gatgatttta aggtctcaca
     2281 cgcaggcaga caaggtgata ttatgcacaa taacaactca cattaacggc aaaagtgcag
     2341 aggtaagaat gacaaatcgt gaagagtggt taagtgcaaa aattgcctat atcaacggac
     2401 tgaaatcacc gtcagagcag caaagattac ttgtgttact ggcagagaaa aaaaaccgaa
     2461 caacgacgga tgaaaaaacg ttgtccgcac tgatacgggc agaaaaaacc gcagagaaag
     2521 cagccgcagc aaaagccagg gtaacggcaa taatagcggc agaacggaag gcagccgcaa
     2581 gggcggaacg aaaggcgcgg gatcatgaac tctacaaggc cgcaggcctc atgattgtgg
     2641 caggactggt ggacagcaaa accggaaaac caaagtttag tgctgccgaa ctggtaggcg
     2701 cactcgcagg aatcgcagag ctaccgcgca accatccaaa atggcaggaa tgggaaaaac
     2761 gcgggaaaga gctactcacc aaagacagtg cgtaagagcc tccgttcacg ttccggttcc
     2821 ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat tgagactgcg gcgagcacaa
     2881 ccaccactca cacacagacg ctaaaaccat tgccaaacga aacacgggaa atcaccccaa
     2941 acctacccgc tcaaaaaaac gcctacaggc gctgttatct gataaggctt gttatctggt
     3001 ctcattttgc gagatctgga tcacatttta gttttcgata gtatgtggca cgactgatcc
     3061 ctaacgtcac ccacggcttc agagttcgtg ccgatgtggc aacggctaca cgctttgatt
     3121 tctgaccttt aatagcctga ttttctgatg aatttgcatt accgccctta cgacctcgag
     3181 cagcctgtac cgcggaaaag ccttcaggag agaatttgcg gtaggtgtat ttcgcaatac
     3241 tcttgccaat agcccgacat tcagccggag aaagcggaac gggaagcgat gcgttgtaca
     3301 tttcgacacg ctgaatcact gcatcaagcc acggtgagaa tgctggccag ccctgacgaa
     3361 tcgccctgta agaccattta cggccctttt cgaacagata ccagtttcgc cccatcccgt
     3421 aatctttatc gacgcgacgg cgcgctgagg cgctcaaatc aagataatcc gccagttcgt
     3481 cgagggtata gagttcccaa cgccattcca ccacctgcca ttcctggtgg aacggatttt
     3541 tgcagatcag gccgctgtag ttcacatccg cgcccagttt ttcacacagc gcacgctcaa
     3601 tcacggcagc gtatttgagc gccgaagccg atgcatccgg cgcggttctc accggaagag
     3661 tgagcgcgta gagcagatgc gcgtgcccgt tacgtggatt ttttacggtg atgttcgggg
     3721 ccggacagtt ccggtcgctc cagtcgatcg ctgcccccgt ccggtcaacg tcaaaggcca
     3781 gccagtgctg cgcatgtggc tggttctgct ggatgaaacg ggcaaggatg gcgatatctt
     3841 tcggtagaat gcgaacgccg tacgtgaaat cgttcgtaca gtacggtttg tgcggtaatt
     3901 tttccctgaa gagttgaagt acggcgctca tgtgttagga tttcctccgt gtgttacgac
     3961 ccacacgccc cttgaatttt cgtgacagtc ttggcggatc ggcacgaatt tcaggggcgt
     4021 ttgtttttca gttggttaca ctacacccaa cggcaaatcc tgtcgatcct tcccgaaaac
     4081 cagacccatt taccataact cctcttgtac gtaccctcta aaacgctctc aatcgctttc
     4141 tgacgcaaaa aactcaaaaa gacaagataa acctagggaa aacacaaaaa acgcgtcaga
     4201 atcgttttta ggggcctgca taactatgca caaaagcgat taatgatcgg tttcacccct
     4261 tcagaagcgc gaactggtca aaacagcaaa ctttctcatc cagaaagcca gctgaactaa
     4321 cccgcatgaa aacaaatttc caaacatatc cagtctggac aacgaaatat cgacaatata
     4381 aaacatcata atcgtgttta tggcgtgtaa aaaagcttac aagagaatta gcattgaaga
     4441 gtcataacaa caaattccaa aaatcaaacc agaggatagg aaaatgaata attttataaa
     4501 tgaatgtatt tccgctccga gaataaacgg catggcagaa tttttctcat caactgcatc
     4561 aggggaaaaa cacaaacctt ttagactagt agaaatagat gatcggcttt ttcattctta
     4621 tgattacatt aagtttgcca aacacctcac aaaaaacagc ggccctttcg ttaatcatta
     4681 tttttgcagt ataccataca gcaaggaaga agagtgcaga ttaggaacaa cagtaataaa
     4741 atacgcaatc acgctaaaca aaaaattaaa cacatggtgt cttggtatgg ctgagggcgc
     4801 aatggcaaga accatttcag aattatcaaa aggaaacata caaacattaa caaccagtcc
     4861 aacaaaagaa aatgaacatg cattttttaa gtatggctca agtgaaaacg cttattttat
     4921 ttgcactcca tttttcctgt tagaggaaag actaaaaaat tcaaatctta aacaatttaa
     4981 tcaaggattc gatattataa tagaagacac cacatttcaa atgtactcac ctaacagatc
     5041 tgggcaaata aaccacgtaa aaaggctatt aaataatgat ggtattttca tatttacaga
     5101 aaaacatagc tcagaacatg atgaatatca aaaaagagaa atccaaaaag attattcatt
     5161 caaacaaagg tatttctcga aagatgagat taacaccaaa aaggaaatcg tactaaacac
     5221 aatgaatttg aatgaagtta gtgttgacga tatgctttcc gcaattaaac aacattttaa
     5281 atacgcatca atatattgga atagcggaaa tttttactca atcgccgcca gcaattcact
     5341 aaaacacata tcaacattta taaatttatt accgaaagct gcaattccta tggaatatgt
     5401 atatgaaaag ctcccacgaa atatatcatg gtaagtaaac atggaatatt caacattatt
     5461 atcatttgct atcgtaacct tatcgcaaac tatatcaata gggcctggcg ttgcattggt
     5521 tataaacaat gccttttcac atggattaaa gtcatcaata aaaacaagta tatacataag
     5581 aattggcgag actatcgtca tggcaatatc tcttttcgcg ttatcctcaa catccagcac
     5641 agaacaacat tttcacatta taaaaatatt cggtggaggc tatttaatat acataggatt
     5701 aatgggttta attaat
//
LOCUS       DAZPAC010000245         5696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZPAC010000245 DAZPAC010000000
VERSION     DAZPAC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.246"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(237..860)
                     /locus_tag="ACHUPM_003841"
     CDS             complement(237..860)
                     /locus_tag="ACHUPM_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR0871500.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1157..1432
                     /gene="ypdI"
                     /locus_tag="ACHUPM_003842"
     CDS             1157..1432
                     /gene="ypdI"
                     /locus_tag="ACHUPM_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR0871501.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(1509..1751)
                     /locus_tag="ACHUPM_003843"
     CDS             complement(1509..1751)
                     /locus_tag="ACHUPM_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR0871502.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            2104..3024
                     /gene="lpxP"
                     /locus_tag="ACHUPM_003844"
     CDS             2104..3024
                     /gene="lpxP"
                     /locus_tag="ACHUPM_003844"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR0871503.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3380..3451
                     /gene="ypdK"
                     /locus_tag="ACHUPM_003845"
     CDS             3380..3451
                     /gene="ypdK"
                     /locus_tag="ACHUPM_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR0871504.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(3516..4754)
                     /gene="alaC"
                     /locus_tag="ACHUPM_003846"
     CDS             complement(3516..4754)
                     /gene="alaC"
                     /locus_tag="ACHUPM_003846"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR0871505.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            5131..>5696
                     /gene="ypdA"
                     /locus_tag="ACHUPM_003847"
     CDS             5131..>5696
                     /gene="ypdA"
                     /locus_tag="ACHUPM_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR0871506.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVL"
BASE COUNT         1451 a         1399 c         1285 g         1561 t
ORIGIN      
        1 tgatttacaa gtaagagtat gattacgctt tttaaagcgt accataaagc ataacataat
       61 aaatgatttg tgatgacact ggattattgc ggtttacttt gaggaaaata aaatgcgtgt
      121 ttatagaacc gcgagcgcca aataatttta tgaccacaac ctgtcgtatt gtggtcataa
      181 aaaattgtcg gctggaggga gttaaatcct ttacctcaat atactgcctg tcagcattag
      241 ttcacaaaca gtgcttcgct atcgacgatg attccatctt ccgcaccttt aagcgccagg
      301 tttgcctcgt agtattgctt tttatcaagc agtttctgcg catcagcaag tgcattacga
      361 gtctgtttaa gcggcatcag atattggttt tccattacgc caacaccggc caggcgaagc
      421 tcttccattg cgcccttttt atcccctttc gccatttttt cattggcaat ttttatcgcg
      481 gcttcttttt cgggtgttgc tacataactc tcagaaattc cgacagatgc attaatgaca
      541 atatattggt catcattgag attggtcttt ttacctggct tagcgaactt tgcccattcg
      601 gtactgtcat ctgacagcaa agcggaagct tcattggtca gttcttttgc cttttccgga
      661 tcgccatgaa acagagccag gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt
      721 tcagagatac gctctgctgc tgccgttttc tgggtttgct gagtttgctg ttgtgccgct
      781 accggtgcat tatcagcagc ccatacagtt gaagatgcca gaattgctgt gaccatcgta
      841 gccataatta aacgtttcat acttacctca ttctttaaca gttttagaaa acgccgccat
      901 catattgggt gagcatgaaa tgagaaatca gagagcaacg gtcattttga aagggttatc
      961 ttacaattgt agttattatc gcaaagcggc atttagtctt acagttgtag gaatatgctt
     1021 cctgtcgcag agaactattc ttatatacca caaagagatg tcatttatta tattgatgta
     1081 aatattgatt aattatatgt aaatacagcc ataacatcgt atggttctca gtgaaccatt
     1141 ttcaggataa ctttctatga aagtaaactt aatacttttc agcttatttt tattggtctc
     1201 tattatggca tgcaatgttt ttgcattttc catttctggt ggtgtaagtg agaggagcta
     1261 taaagagact gaaaaaacat cagcgatgac aaccacacac tctacaaaac ttcagccatc
     1321 acaggcgatt ttgtttaaga tgagagaaga tgcgccacca ttaaacctca cagaagaaat
     1381 cacgcctact tatccgacaa aggcgaatta tcttattcat cctgtgcgat agcgtctgtc
     1441 acatggcagg tgaacaatca tgtctgatgc tgatgtgaac acctgcccaa atgagccatc
     1501 ctgacccact atcgcctgcc ataccccacc ataaagcgaa tcatcaagcc acaagccggg
     1561 gcaagaaaca taaatatccc cgtcaccagt tcattaccgc cagttaacca catggtgaag
     1621 taataaatga gtgcagcgag gatcaccatg ccgaagaaag aagagaccgc cgataccacg
     1681 ttcagtacct gaccgatgta gccgcttacg cagacaatac acagggcgag aaacatccat
     1741 aaataaatca tagctcaacc tcctgccgcg ctaaaaaacc tgtaacttca gtcactttaa
     1801 tagtaagaca atccttaacc tctggcaatt tttgctaagc atcaatgaaa acagcacgtt
     1861 aaatgcaacg ctattgcgtg cggaaaattt tttactttgc acgattaata atcagtagct
     1921 gaaagcagtc agccagtgat aaagtagagc caccgcattc atgctcctcg tggttatgtc
     1981 ctgactagtc tttacactct ttacaggaac cattgtcgta catgatggcc caaccaattg
     2041 agtgtaccgc tgtcgacatg gaatcttctg cgatacaaca attcgtatct acagaaggta
     2101 actatgtttc cacaatgcaa attttcccgc gagtttctac atcctcgcta ctggctcaca
     2161 tggtttgggc ttggtgtact ctggctttgg gtacagcttc cttatcctgt tctctgcttt
     2221 ctcggcacgc gtattggcgc aatggcgcga ccattcctga aacgtcgtga atctatcgcc
     2281 cgtaaaaacc tggaactttg tttcccgcag cattctgcgg aagaacgcga gaagatgatt
     2341 gccgaaaact ttcgttcact cggcatggcg ctggtagaaa ccggcatggc atggttctgg
     2401 cccgacagtc gcgtacgtaa atggtttgat gttgaagggt tggataaccc taaacgcgca
     2461 caaatgcaaa atcgcggcgt aatggttgtc ggcgtccatt ttatgtcgct ggaactgggc
     2521 ggccgcgtga tgggactgtg ccaaccaatg atggctacct atcgtccaca taataatcag
     2581 ctgatggaat gggtgcagac ccgtgggcgc atgcgctcta acaaagcgat gatcggcaga
     2641 aataatctgc gcggcattgt cggtgcactg aagaaaggtg aagcggtatg gtttgctccc
     2701 gatcaggatt atggtcgtaa aggcagctcc ttcgcgccgt tctttgcggt ggaaaatgtc
     2761 gccacaacca atggcaccta tgttctctcc cgtctctctg gcgcagccat gttgaccgta
     2821 acgatggtaa gaaaagcgga ttacagcgga tatcgtttgt ttatcacccc agagatggaa
     2881 ggctacccga cagatgaaaa tcaagccgct gcctatatga acaagattat cgagaaagag
     2941 atcatgcgcg caccggagca gtacctctgg atccaccgtc gctttaaaac gcgcccggtg
     3001 ggagaatcgt cgttgtacat ttaacaaact gctcttactc tttccctcct caaatcggcc
     3061 ataataacct catggttttt atggcttttt tcttaaggtt ttttccttcg ctgaatatct
     3121 aattattacc gtcagaaatt aaactgtcgc cgatatgaga cacttgtaag ttactaaaag
     3181 taaataagaa aatgcctctt gtctcgtttc agttttaggc gtacattaac gccgtcttga
     3241 gcagtttagc tcagaattct ggggtgatgc ccacggtaaa atacagggct tttaaggtcg
     3301 ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa
     3361 ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta tggtcatcgt
     3421 ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc
     3481 catcaggctc ctgtttgcat ttctgacgac ggtgtttatt ccgcgttttc gtgaatatgt
     3541 ttgctgctgg cgggtagtaa accgtcggca cggaacatcg ctttaatccc tctgatggcc
     3601 tgacgaatac ggtcgcggtt ttcaatcagt gcaaagcgaa catgcgtatc accatagtcg
     3661 ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt taaggagctt cttggcaaat
     3721 tccagcgatc ccatggccgc atatggttcc gggattttcg cccagacata catcgaagcc
     3781 ttcggcattt cgaccatcca gcccgcttca tgcagccctt taaccaatac atcacggcgg
     3841 cgtttgtact gttcggtaat gtcgcgcacg cactgttgat cgccctcgag tgccgcaatc
     3901 gccgccacct gcaacggcgt aaaagtgccg taatcgtgat agcttttgat tcgcgccagg
     3961 gcactgacca gcgttttgtt gccgaccata aagccgatac gccagcccgc catgttgtag
     4021 cttttcgaca gcgtaaagaa ctcgaccgcc acatcgcgtg cacccggcac ctgcataatt
     4081 gacggcgctt tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc
     4141 acatcgtagc gtttcgccag cgccacaacc ttttcgaaga actccagttc cacgcattgc
     4201 gcggttgggt tcgacgggaa gccgaggatc atcatcttcg gtttcggata actttcacga
     4261 atggcgcgct ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc
     4321 tgcgccccgg caatcaccgc gccataaata tggatcgggt aacttggatt cggcaccagc
     4381 accgtgtcac catgatccag cgtcgccagc atcagatgcg ccaggccctc tttcgaacca
     4441 atagtgacga tggcttctga ttccgggtcg atttcaacgt cgtagcgatc ctgataccag
     4501 cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc
     4561 gggcgctggg ccacagtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg
     4621 ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca
     4681 gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg
     4741 cgagtgtcag ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct
     4801 gccacaatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc agcgttttag
     4861 aacattttta tccatggctt taaatgagga actccacttc aaaaacagga aatttttcta
     4921 cgctaacaag aaaattacca ccaattttat taacattata atttcaataa atttaccagc
     4981 cagaagtgca ttatcaagag atacattgct aatacaccat ttatctttca atcccctttt
     5041 aaatttatgg tttttcctca aaaacggcct gctggtcatc agccttcagg ttacctatca
     5101 tagaggttta atccttattc agagtcaccc gtgcacgaaa tattcaacat gctgctggcg
     5161 gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct ttctcatccg tatccgcctg
     5221 tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc cgttaccgcc
     5281 attttctcgc tgttcgccct gttcagcacc tggtccggcg ttcccgtaga aggctcgctg
     5341 gtgaacgtac gtattatcgc ggtgatgtcc gacgggattc tgtttggccc gtgggtaggc
     5401 atcattaccg gcgtgattgc gggtattcac cggtatttaa ttgatatcgg cggcgtgacg
     5461 gcgatcccct gctttatcac cagcattctg gcgggttgta tatcaggctg gataaacctg
     5521 aaaatcccca aagcacagcg ctggcgcgtc agtattctcg gcggcatgtt gtgtgagacg
     5581 ttgaccatga ttctggtaat tgtctgggca ccaactaccg cgttggggat cgatatcgtc
     5641 tctaaaatcg gcattccaat gatcctcggt agcgtctgta tcggctttat tgtgct
//
LOCUS       DAZPAC010000246         5692 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZPAC010000246 DAZPAC010000000
VERSION     DAZPAC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.247"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..828
                     /gene="yneK"
                     /locus_tag="ACHUPM_003848"
     CDS             <1..828
                     /gene="yneK"
                     /locus_tag="ACHUPM_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR0871507.1"
                     /translation="RHKENIEFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRV
                     FQTGVERVLFLFLNDFIEQFPMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLV
                     LNFISRGNFVILPRLWNNSEVHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGS
                     NKHVLPEVCICFLFLLAEQESPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASL
                     SDDKIIKHMARRTLELFVKNETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            978..2168
                     /gene="ydeA"
                     /locus_tag="ACHUPM_003849"
     CDS             978..2168
                     /gene="ydeA"
                     /locus_tag="ACHUPM_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR0871508.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2193..2858)
                     /gene="marC"
                     /locus_tag="ACHUPM_003850"
     CDS             complement(2193..2858)
                     /gene="marC"
                     /locus_tag="ACHUPM_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR0871509.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3070..3504
                     /gene="marR"
                     /locus_tag="ACHUPM_003851"
     CDS             3070..3504
                     /gene="marR"
                     /locus_tag="ACHUPM_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR0871510.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3524..3907
                     /gene="marA"
                     /locus_tag="ACHUPM_003852"
     CDS             3524..3907
                     /gene="marA"
                     /locus_tag="ACHUPM_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR0871511.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3939..4157
                     /gene="marB"
                     /locus_tag="ACHUPM_003853"
     CDS             3939..4157
                     /gene="marB"
                     /locus_tag="ACHUPM_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR0871512.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4214..5653)
                     /locus_tag="ACHUPM_003854"
     CDS             complement(4214..5653)
                     /locus_tag="ACHUPM_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR0871513.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         1495 a         1401 c         1257 g         1539 t
ORIGIN      
        1 agacacaaag aaaatattga atttcaggta gtaataaatt taagccctat ttatttagat
       61 aattttcgcc atgatggaga aattaatatt ttttgcgctc ccaacccaaa gcctgtcact
      121 atggggcgcg tatttcagac cggcgtcgag cgtgtgctct ttctgtttct gaatgatttt
      181 attgaacaat ttccaatgtt caaccctggc gttccgatca aaatagcgca tacaccacat
      241 attgaacccc tgcctccgga tcaccatacc gctgcagatt acttacgcca gtttgatttg
      301 cttgtcctga attttatctc tcgcggtaat tttgtcatac tcccccgatt atggaataac
      361 tctgaggttc acagatggtt cgtcaataaa gatcctaact tgatcaccgc cattctcgac
      421 ataacggaca gcgaattaaa agaggattta ttgcaaagcc tgatggattc attaggttct
      481 aacaaacatg tactacccga agtctgcatc tgctttttat tccttttagc agaacaagaa
      541 tctcctcatt ttcaggactt gtttttattt ttcgccaata tgttattgca ctatcaccaa
      601 tttatgaatc ccaatgaaag tgatttgaat gacgtgttga tgccagcatc attaagtgat
      661 gataaaatta tcaagcatat ggcgcgcagg accctcgaac tgtttgtaaa aaatgaaaca
      721 ccgccaaaag ttactcacga agacctggtg aaaaacaggc ctcgctcccc tgtcagacca
      781 cctatacccg caacctccaa aacgccagac ctccctgaac gtcattaaac cgtgatgtta
      841 ccgactctct gacgcggaac agaatcagcg tcagagaaac ggaaaacgcg atccagatca
      901 caaatacatt gtattcacat cattaaccgt tttaagatca tttcatcact ttttcgcaac
      961 tcacccgata atctgttatg acaacaaaca ctgtttcccg caaagtggcg tggctacggg
     1021 tcgttacgct ggcagtcgcc gccttcatct tcaacaccac cgaatttgtc cctgttggcc
     1081 tgctctctga cattgcgcaa agttttcaca tgcaaaccgc tcaggtcggc atcatgttga
     1141 ccatttacgc atgggtagta gcgctaatgt cattgccttt tatgttaatg accagtcagg
     1201 ttgaacggcg caaattactg atctgcctgt ttgtggtgtt tattgccagc cacgtactgt
     1261 cgtttttatc gtggagcttt accgttctgg tgatcagtcg cattggtgtg gcttttgcac
     1321 atgcgatttt ctggtcgatt acggcgtctc tggcgatccg tatggctccg gccgggaagc
     1381 gggcacaggc cttgagttta attgccaccg gtacagcact ggcgatggtc ttaggtttac
     1441 ctctcgggcg cattgtgggg cagtatttcg gttggcgaat gaccttcttc gcgattggta
     1501 ttggggcgct tatcaccctt ttgtgcctga ttaagttact tcccttactg cccagtgagc
     1561 attccggttc attgaaaagc ctcccgctat tgttccgccg cccggcattg atgagcattt
     1621 atttgttaac tgtggtggtt gtcaccgccc attacacggc atacagctat atcgagcctt
     1681 ttgtgcaaaa cattgcggga ttcagcgcca actttgccac ggcattactg ttattactcg
     1741 gtggtgcggg cattattggc agcgtgattt tcggtaaact gggtaatcag tatgcgtctg
     1801 cgttggtgag tacggcgatt gcgctgttgc tggtgtgtct ggcactgctg ctacctgcgg
     1861 cgaacagtga aatacacctc ggggtgctga gtattttctg ggggatcgcg atgatgatca
     1921 tcgggcttgg tatgcaggtt aaagtgctgg cgctggcacc agatgctacc gacgtcgcga
     1981 tggcgctatt ctccggcata tttaatattg gaatcggggc gggtgcgttg gtaggtaatc
     2041 aggtgagttt gcactggtca atgtcgatga ttggttatgt gggcgcggtg cctgcttttg
     2101 ccgcgttaat atggtcaatc attatatttc gccgctggcc agtgacactc gaagaacaga
     2161 cgcaatagtt gaaaggccca ttcgggcctt ttttaatggt acgttttaat gatttccagg
     2221 atgccgttaa taataaactg cacacccata cataccagca ggaatcccat cagacgggag
     2281 atcgcttcaa tgccaccctt gcccaccagc cgcataattg cgccggagct gcgtaggctt
     2341 ccccacaaaa taaccgccac caggaaaaag atcagcggcg gcgcaaccat cagtacccaa
     2401 tcagcgaagg ttgaactctg acgcactgtg gacgccgagc taataatcat cgctatggtt
     2461 cccggaccag cggtacttgg cattgctagc ggcacaaagg caatattggc actgggttca
     2521 tcttccagct cttccgactt gcttttcgcc tccggtgaat caatcgcttt ctgttgcgga
     2581 aagagcatcc gaaaaccgat aaacgcgacg attaagccgc ctgcaattcg cagaccggga
     2641 atcgaaatgc caaatgtatc catcaccagt tgcccggcgt aatacgccac catcatgatg
     2701 gcaaatacgt acaccgaggc catcaacgac tgacgattac gttcggcact gttcatgttg
     2761 cctgccaggc caagaaatag cgcgacagtt gttaatgggt tagctaacgg cagcaacacc
     2821 accagcccca ggccaattgc tttaaacaaa tctaacattg gtggttgtta tcctgtgtat
     2881 ctgggttatc agcgaaaagt ataaggggta aacaaggata aagtgtcact ctttagctaa
     2941 ccttgcatcg cattgaccaa aacttgaacc gatttagcaa aacgtggcat cggtcaattc
     3001 attcatttga cttatacttg cctgggcaat attatcctct gcaactaatt acttgccagg
     3061 gcaactaatg tgaaaagtac cagcgatctg ttcaatgaaa ttattccatt gggtcgctta
     3121 atccatatgg ttaatcagaa gaaagatcgt ctgcttaacg agtatctgtc tccgctggat
     3181 attaccgcgg cacagtttaa ggtgctctgc tctatccgct gcgcggcgtg tattactccg
     3241 gttgaactga aaaaagtgtt gtcggtcgac ctgggagcac tgacccgtat gctggatcgc
     3301 ctggtctgta aaggctgggt tgaaaggttg ccgaacccga atgataagcg cggcgtactg
     3361 gtaaaactta ccaccagcgg cgcggcaata tgcgaacaat gccatcaatt agttggccag
     3421 gacctgcatc aagaattaac aaaaaacctg acggcggacg aagttgcaac acttgagcat
     3481 ttgcttaaga aagtcctgcc gtaaacaaaa aagaggtatg acgatgtcca gacgcaatac
     3541 tgacgctatt accattcata gcattttgga ctggatcgag gacaacctgg aatcgccact
     3601 gtcactggag aaagtgtcag agcgttcggg ttactccaaa tggcacctgc aacggatgtt
     3661 taaaaaagaa accggtcatt cattaggcca atacatccgc agccgtaaga tgacggaaat
     3721 cgcgcaaaag ctgaaggaaa gtaacgagcc gatactctat ctggcagaac gatatggctt
     3781 cgagtcgcaa caaactctga cccgaacctt caaaaattac tttgatgttc cgccgcataa
     3841 ataccggatg accaatatgc agggcgaatc gcgcttttta catccattaa atcattacaa
     3901 tagctagttg ataacgtgac aacgtcactg aggcaatcat gaaaccactt tcatccgcaa
     3961 tagcagctgc gcttattctc ttttccgcgc agggcgttgc ggaacaaacc acgcagccag
     4021 ttgttacttc ttgtgccaat gtcgtggttg ttcccccatc gcaggaacaa ccaccgtttg
     4081 atttaaatca catgggtact ggcagtgata agtcggatgc gctcggcgtg ccctattata
     4141 atcaacacgc tatgtagtta ttgtcaggct ctgatcacag agcctgacat ctccgtaata
     4201 tcttcctcaa gtctcaaata ttttcgccat tgttggcgat gagatcccga taccagtaaa
     4261 acgacgcttt acgtatccgt tccagcgttc ccttcccttc gttgtcttta tcgacataaa
     4321 tcattccgta gcgctttttc atttcccctg ttccggcaga aaccaggtca atgcagcccc
     4381 acggggtgta gccaattaag tcaacaccat cttcaactac ggcttttttc atttcccgaa
     4441 tatgggaagc aaagtaatca atgcgatagt gatcgttcac cgtgccgtca gcttgtctct
     4501 ggtcaaccgc accaaatcca ttttcgacaa taaacagcgg caactggaaa tgatcccaga
     4561 accagttgag tgaataacgt agccctgccg gatcaatttg ccatccccat tcggatgtat
     4621 cgatataagg gttgctgacc aaatctcgtg gttcaacata atccagttgc gggttatcgg
     4681 gagaaaattg tgtcgtaaaa gacatgtagt agctaaaacc gataaagtcg acacatccac
     4741 tggcaagaat cgcgttatct tctggtgtga tatcgaggtt gaatcctttc ctggcaaagt
     4801 aattcagcat atgttgcgga taatatccac gagcatgaac atcagtaaac cagtaacgac
     4861 gatgcatcgc tttcgtggcc atcatcatat cgttgggtgc acacgtcaga ggatagatgg
     4921 ggcacatggc gatcatacag ccaatgttaa attcaggatt gatcgatttt ccagtctgta
     4981 ccgccagggc actggcaact aactcgtaat gtaccgcctg gtacattatt tgttcgcgct
     5041 cattgatatc ttcctctggc gaatacaaga taccggaatt agtaaatgga cacaggcttt
     5101 cgctgaaatt cacctgatta ttgatttcgt taaacgtcat ccagtacttt actttttctt
     5161 tatagcgcgt gaagaccgtt gatgcgaagc ggataaaaaa gtcgatcagc ttacggtttc
     5221 gccagccacc atattttgtc accagatgat aaggcatctc aaaatgcgaa agcgtcacca
     5281 caggttccat tccttgcttc aggcattcat cgaacagatc atcataaaat tgtaaaccct
     5341 cttcattcgg ctcctgttcg tcaccttgcg gaaagattcg tgtccaggca atggaagttc
     5401 gaaagcattt gaatcccatc tcggcaaata actgaatatc tgttttatag cgatgataaa
     5461 aatcaattgc ttcatgattg ggataattaa gcccgtcgat aacgccttct gtcacttcac
     5521 gcggcacccc gtgagcgcca gcagtcatca catcagcgat actgatgcct tttcctcctt
     5581 cattccagcc accttccaac tgatgcgcgg ctaccgcgcc accccataaa aaaccttttt
     5641 taaatcctga catacacaac tccttaacta aaatagatta ccaccagact tc
//
LOCUS       DAZPAC010000247         5640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZPAC010000247 DAZPAC010000000
VERSION     DAZPAC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.248"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHUPM_003855"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHUPM_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR0871514.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHUPM_003856"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHUPM_003856"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR0871515.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHUPM_003857"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHUPM_003857"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR0871516.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHUPM_003858"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHUPM_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR0871517.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1351 c         1529 g         1359 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAZPAC010000248         5631 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZPAC010000248 DAZPAC010000000
VERSION     DAZPAC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.249"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..706
                     /locus_tag="ACHUPM_003859"
     CDS             <1..706
                     /locus_tag="ACHUPM_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0871518.1"
                     /translation="KKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFS
                     LESVFILVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVA
                     GVLSAAFYVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVF
                     SSGILQYFLAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFI
                     VTVLSVYFLNRKYSEE"
     gene            complement(1909..2100)
                     /locus_tag="ACHUPM_003860"
     CDS             complement(1909..2100)
                     /locus_tag="ACHUPM_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR0871519.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENR"
     gene            2165..2488
                     /locus_tag="ACHUPM_003861"
     CDS             2165..2488
                     /locus_tag="ACHUPM_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR0871520.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAQPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     ARDDS"
     gene            2697..3128
                     /locus_tag="ACHUPM_003862"
                     /pseudo
     CDS             2697..3128
                     /locus_tag="ACHUPM_003862"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3166..3696
                     /locus_tag="ACHUPM_003863"
     CDS             3166..3696
                     /locus_tag="ACHUPM_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR0871521.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETN
                     WTKDNGKLNAVRLVQE"
     gene            3703..3936
                     /locus_tag="ACHUPM_003864"
     CDS             3703..3936
                     /locus_tag="ACHUPM_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR0871522.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDN
                     AMLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
     gene            4417..5469
                     /locus_tag="ACHUPM_003865"
     CDS             4417..5469
                     /locus_tag="ACHUPM_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871523.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLR
                     KWVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARR
                     GEVSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKD
                     GLKIYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALA
                     DIRNMTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPG
                     ELTKDERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSF
                     QPRKFENKPLYEKEDD"
     gene            5483..>5631
                     /locus_tag="ACHUPM_003866"
     CDS             5483..>5631
                     /locus_tag="ACHUPM_003866"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFR0871524.1"
                     /translation="MLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVILSWI
                     VLFIK"
BASE COUNT         1487 a         1153 c         1514 g         1477 t
ORIGIN      
        1 aaaaaaactc ccattaaact caaaagcgat acttgcttat gcgacaggcg tttttgaacc
       61 attcttagcg tatactttta cactctacgg actttatttt atttcagcag gaagcgcatc
      121 aattattttt tcacttgaat cagtgtttat attggtgttg tctgttctta tttgctcagt
      181 taaaattaat tccccgcaat attttatatt gttccttata ggtgcaattg ttggcagtat
      241 tttggtagcc ttgccagaca tcagtaatgg atcgaataat attgttggat attgtcttgt
      301 tgttgccggt gtgttatcgg cagcatttta tgttgttata tcgtctcgcc ttattaaatc
      361 gtttgagcct atcacattat tgagcggaca gttgacattc tcccttatac tgtcatcgct
      421 ctttattttt atatctaaat catcaattta tcttccgttg gaatccgtga ttattgtttt
      481 ttcatctgga atattacaat attttttagc tttttgtttt tacttgaact ccttgcgttg
      541 gctcccagtt catttggcag ggataatgtt gtactttatt ccgctagttg cattattttt
      601 gtcatggttg tttttggatg aacaattcac attcttacag ggggtaggat ttattgtaac
      661 tgtgctaagt gtatattttc ttaacagaaa atactcagag gagtaacatt ctgttcagga
      721 ttgccccccg gcctccatcg ataccgtgcc aaatgcacgt aaaaaacggg agcgtttctg
      781 gtgtcggaat gtaaccaagg gggtcagagg cgcgctaaaa cgctctcagg ggagtcaaat
      841 agagacgttt gtaacaaaaa gatcaaagga tcctcttgag atcctttttt tctgcgcgta
      901 atctgctgcc tgtaaacgaa aaaaccgcct tgcagggcgg tttgatcgaa ggttaagtca
      961 gcttgcagac tgcttaacca ggtaactgtc ttatgcgaga cgagtcacca aaattgttct
     1021 tctagtgtag ccgcactccg gcagccactt caagtactct ctaaatatct cgcacatgac
     1081 ttttaccaat ggctgctgcc agtggcgctt tgtggtgtct tttccgggtt ggacccaaga
     1141 gaacagttac cggagaaggc gcagcagtcg ggctgaacgg ggggttcctg catacagccc
     1201 agcccggagc gaactgccta agtcgccccg agataccaga agacgtgagc tatgagaaag
     1261 cgccacgtgt cccgtaaggg agaaaggcgg aaaaggtatc cggtaatcga tatggcggga
     1321 acgtgggggc gcaggaggga gcgattcacc ggaaacggtg atgatcttta agccacgtcg
     1381 ggtttcgcca ccgctggcgg atttcatggt tgagccgcaa ggctcccaca gatgcaccga
     1441 aaaagtgtct gttttgtgaa gtccgtcagg ggggcggagc ctatggaaaa agcgcctccg
     1501 gcgcggcctt tcccggggtt tcgttttttg cgcacatgtt ctttcctggt ttatccccgg
     1561 gttctgtgga taacagggtt agcgtttttg agaaaggaga gtccgctcgc cgcagccgaa
     1621 cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga gcgcctgaac tggcattttc
     1681 tccttacgca tctgtgcggc atttcacacc ggacatggcg cacttttcat acaatccgca
     1741 ctgatgccga atggttaagc cagtatacac tccgctatcg ctacgtgact gggtcagggc
     1801 ttcgccccga caccccaaag gtcaacggcc tgacgcgccc tggcgggctt gtctgctccc
     1861 ggcatccgct cacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agcggttttc
     1921 accgtcatcc ccgaagcgtg cgaggcaact gcggtaaagc tcatcggcgt ggtcgtgaag
     1981 cgattcacag atgtctgcct gttcatccgc gtccagttcg ttgagcttct ccagcagcgt
     2041 taatgtctgg cctctgatga agcgggccat gttaagggcg gttttttcct gtttggtcac
     2101 tgatgcctcc gtgtaagggg gggttctgtt catgggggta atgataccga tgaaacgcga
     2161 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgctgtga
     2221 gggtaaacaa ctggcggtat ggatgcggcg ggtctgcctg ggtgagccgg ttgcccgttc
     2281 cgggaagctg ccgacactgg cacagccgtt actgcgtcag ctggccgcca tcgggaataa
     2341 ccttaaccag acagcgagga aggtgaacag cgggcagtgg tcttccggtg accgggttca
     2401 ggtggtggcc gcactgatgg ccatcgggga tgagctgcgc cggctgcgtc tggcggtcag
     2461 ggaacagggg gcgcgggatg atagttaagt ttcatgccag gggaaaaggt ggtggcagtg
     2521 gtccggttga ttacctgctg ggaagggagc gtaaccggga aggggcaacg gtgcttcagg
     2581 ggaatccgga agaggtccgg gaactcatcg atgccacgcc atttgcgaag aaatacacgt
     2641 caggtgttct gtcgttcgcg gagaaggagc tgccgcctgg agggcgtgaa aaagtgatgg
     2701 cgagctttga gcgtgtactg atgcccggtc tcgagaaaaa tcagtacagc atcctgtggg
     2761 tggagcacca ggacaaggga cggcttgagc tgaattttgt cattccgaac atggagctac
     2821 agaccggaaa acgcctccag ccgtactacg accgggcaga caggccgaga attgatgcct
     2881 ggcagacgct ggtgaatcac cattacggtc tgcatgaccc caacgccccg gagaaccgcc
     2941 ggacgctgac ccttcctgat aacctgccgg aaacgaaaca ggcgcttgct gagggcgtca
     3001 cacgcgggat agacgcgctt taccatgccg gagagataaa aggccgtcag gatgtgattc
     3061 aggcgctcac agaggcgggg cttgaagtgg tcagggtgac gcgaagcagt atcagcattg
     3121 cagacccgaa cggcgggagg aatatcagac tgaaaggagc gttttatgag caatctttta
     3181 cagacgggcg cggagttcga gaaaaagctg aaagagagag ccgaatctac agagaaaatg
     3241 ctgaacgacg agttcaggaa gctcgaagaa tctgtaaaca gggaactgac ctcaaacgaa
     3301 tctttaatca ggaacgctat caacgaccat acaacagccc tgaaagaact gctggagagg
     3361 tatcagaaaa cgaccgtcga tacgatggac gcgcactgga aaaccgtact gaaaatgagc
     3421 gtgaaacgct ggctgtggct gattatcgtc tcagtactga tgtttgccac gaccgggagt
     3481 ctgctgtggt accagggcat gaagataaac gcgaatatga atattctccg cgagcagaaa
     3541 gagagtctgg agaagctgaa cgcgaagaca tggggcgtgc gttatcacga ggacagcaac
     3601 ggacgttttc tggtgttgcc gaaggggatg aaagccgaaa cgaactggac gaaggacaac
     3661 ggcaaactga acgcagtgag actggtgcag gagtgacaga gcatgacgga gctggaaaga
     3721 catttgctga acgcattaga gcaactgcaa caggactata tgcagcggct gaacgaatgg
     3781 gagaacgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga caacgcgatg
     3841 ctgaacgggc gagtcatgca gttgagtcag caggtgcagc acttgagcga gcggacagaa
     3901 cgcttgagcc agttatacag cgagaacagg agataaggga tgaacggctg atgcaggaac
     3961 gcgaacatga gttatctctg gagcgggagc gtcagccgga aatccaggag cggacactgg
     4021 atggcccttc gctgggatgg tgaaaccatg aaaaatgacg tgttcagtgg attaaggcgg
     4081 gctaatgtgg cctgtagcat ctggttgcat aggtattcat gcggttaaaa tttatcaggt
     4141 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     4201 gcaccgaacg cgttttggcg gtgcgtacaa tgcgacgtta tggtaaatgg agcggatttt
     4261 caggaaggag tgatactaac aactcgaaca ctgtgcaaat aatgattttt ttaaaatatc
     4321 ggtaacgata ataaattatt tcgttttacg ccggtaataa atataatgta gtttcagtat
     4381 ataaaaaaat atatgaggtg tcttgtggga gctttcatga atcttcaaca acgtataaat
     4441 aaactaccac aattatcatc cagtttttca tttggaaaag atattgataa tattcattca
     4501 tttatcttta acgaaacttc taaagataaa attgaagatt tactaagaaa atgggtgtca
     4561 ggcaatcagc cctgtgtatt tggtaaattg gcaagcaaga aaattaaagg gcttgatttc
     4621 catttatcta ttgtaaatag cccccaacta tataatgatg atggtcattt gtttgatttt
     4681 ttgcgtaatg aaagagttcg ttttaaagaa cgcgcaagaa gaggagaggt cagtgcgcat
     4741 ctcatttatt ttattcatcc acaacttgcc tttgctcgtc catcagaaga gttagtggac
     4801 atacaaaaat atatttgttc gctgcatatg ccagagtgct atccaataaa agaggatgtt
     4861 atttatactg aaagcgtacc atttcaggat aaagatgggc taaaaatata taaggctggt
     4921 gttaatgttt tttactcttc tgctcataga acaagaaatc atgatagaag aataccaggg
     4981 ggaatactta tttctgtaaa tgccccgggg cattttatga ggctggcgat agaaaaaggt
     5041 ttctataaag atcaagaaca agcgttagcg gatattcgta acatgactat tcagtctgtt
     5101 ggtaagggtg ggtattcaca tccggaaggg atttctacaa catggcattc agaatctaat
     5161 ttggatcgtt ttggatgccc agtgcacacc ggtaatagtt cttattacag cggtttttat
     5221 catactgatg tattaattcc aggagaatta actaaggatg aaagattgct ccatgaaata
     5281 gaaaatagtg atccaatgat cttcaactgg aatgtattgt tttatgtttc attagaagag
     5341 tttcctatag atgatcctta ttatggagag tttattggta ttcctgtaga tgatgcatct
     5401 atgtttttta actcctttca accaaggaaa tttgaaaaca aaccccttta tgagaaagag
     5461 gatgattaat atgattaaag gaatgctttt tgctttttta gcagcgttct catggggggc
     5521 agctatcgta atgagcaaaa aaggagttga agaactagat gccggagggg tgtttttctg
     5581 gcagatttgt agtgcagtaa tactatcttg gatagttctt tttataaaaa g
//
LOCUS       DAZPAC010000249         5620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZPAC010000249 DAZPAC010000000
VERSION     DAZPAC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.250"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..513)
                     /locus_tag="ACHUPM_003867"
     CDS             complement(<1..513)
                     /locus_tag="ACHUPM_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR0871525.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="ACHUPM_003868"
     CDS             complement(759..1211)
                     /locus_tag="ACHUPM_003868"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0871526.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHUPM_003869"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHUPM_003869"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR0871527.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHUPM_003870"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHUPM_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR0871528.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="ACHUPM_003871"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="ACHUPM_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHUPM_003872"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHUPM_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR0871529.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5620)
                     /locus_tag="ACHUPM_003873"
     CDS             complement(5494..>5620)
                     /locus_tag="ACHUPM_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0871530.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1468 c         1460 g         1353 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgatgtgc
//
LOCUS       DAZPAC010000250         5616 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZPAC010000250 DAZPAC010000000
VERSION     DAZPAC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.251"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            162..926
                     /gene="pdhR"
                     /locus_tag="ACHUPM_003874"
     CDS             162..926
                     /gene="pdhR"
                     /locus_tag="ACHUPM_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR0871531.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            1087..3750
                     /gene="aceE"
                     /locus_tag="ACHUPM_003875"
     CDS             1087..3750
                     /gene="aceE"
                     /locus_tag="ACHUPM_003875"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR0871532.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            3765..>5616
                     /gene="aceF"
                     /locus_tag="ACHUPM_003876"
     CDS             3765..>5616
                     /gene="aceF"
                     /locus_tag="ACHUPM_003876"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR0871533.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITI"
BASE COUNT         1421 a         1432 c         1552 g         1211 t
ORIGIN      
        1 ttttgaaacg ttatatatgt caagttgtta aaatgtgcac agtttcatga tttcaatcaa
       61 aacctgtatg gacataaggt gaatactttg ttactttagc gtcacagaca tgaaattggt
      121 aagaccaatt gacttcggca agtggcttaa gacaggaact catggcctac agcaaaatcc
      181 gccaaccaaa actctccgat gtgattgagc agcaactgga gtttttgatt ctcgaaggca
      241 ctctccgccc gggcgaaaaa ctcccaccgg aacgcgaact ggcaaaacag ttcgacgtct
      301 cccgtccctc cttgcgtgag gcgattcaac gtctcgaagc gaagggcttg ttgcttcgtc
      361 gccagggtgg cggcactttt gtccagagca gcctatggca aagcttcagc gatccgctgg
      421 tggagctgct ctccgaccat cctgaatcac agtatgactt gctcgaaaca cgacacgccc
      481 tggaaggtat cgccgcttat tacgccgcgc tgcgtagtac cgatgaagac aaggaacgca
      541 tccgtgaact ccaccacgcc atagagctgg cgcagcagtc tggcgatctg gacgcggaat
      601 caaacgccgt actccagtat cagattgccg tcaccgaagc ggcccacaat gtggttctgc
      661 ttcatctgct aaggtgtatg gagccgatgt tggcccagaa tgtccgccag aacttcgaat
      721 tgctctattc gcgtcgcgag atgctgccgc tggtgagtag tcaccgcacc cgcatatttg
      781 aagcgattat ggccggtaag ccggaagaag cgcgcgaagc atcgcatcgc catctggcct
      841 ttatcgaaga aattttgctc gacagaagtc gtgaagagag ccgccgtgag cgttctctgc
      901 gtcgtctgga gcaacgaaag aattagtgat ttttctggta aaaattatcc agaagatgtt
      961 gtaaatcaag cgtatataaa agcgcggcaa ctaaacgtag aacctgtctt attgagcttt
     1021 ccggcgagag ttcaatggga caggttccag aaaactcaac gttattagat agataaggaa
     1081 taacccatgt cagaacgttt cccaaatgac gtggatccga tcgaaactcg cgactggctc
     1141 caggcgatcg aatcggtcat ccgtgaagaa ggtgttgagc gtgctcagta tctgatcgac
     1201 caactgcttg ctgaagcccg caaaggcggt gtaaacgtag ccgcaggcac aggtatcagc
     1261 aactacatca acaccatccc cgttgaagaa caaccggagt atccgggtaa tctggaactg
     1321 gaacgccgta ttcgttcagc tatccgctgg aacgccatca tgacggtgct gcgtgcgtcg
     1381 aaaaaagacc tcgaactggg cggccatatg gcgtccttcc agtcttccgc aaccatttat
     1441 gatgtgtgct ttaaccactt cttccgtgca cgcaacgagc aggatggcgg cgacctggtt
     1501 tacttccagg gccacatctc cccgggcgtg tacgctcgtg ctttcctgga aggtcgtctg
     1561 actcaagagc agctggataa cttccgtcag gaagttcacg gcaatggcct ctcttcctat
     1621 ccgcacccga aactgatgcc ggaattctgg cagttcccga ccgtatctat gggtctgggg
     1681 ccgattggtg ctatttacca ggctaaattc ctgaaatatc tggaacaccg tggcctgaaa
     1741 gatacctcta aacagaccgt ttacgcgttc ctcggcgacg gtgaaatgga cgaaccggaa
     1801 tccaaaggtg cgatcaccat cgctacccgt gaaaaactgg ataacctggt cttcgttatc
     1861 aactgtaacc tgcagcgtct tgacggcccg gtcaccggta acggcaagat catcaacgaa
     1921 ctggaaggca tcttcgaagg tgctggctgg aacgttatca aagtgatgtg gggtagccgt
     1981 tgggatgaac tgctgcgtaa agataccagc ggtaaactga tccagctgat gaacgaaacc
     2041 gttgacggcg actaccagac cttcaaatcg aaagatggtg cgtacgttcg tgaacacttc
     2101 ttcggtaaat atcctgaaac cgcagcactg gttgcagact ggactgacga gcagatctgg
     2161 gcactgaacc gtggtggtca cgatccgaag aaaatctacg ctgcattcaa gaaagcgcag
     2221 gaaaccaaag gcaaagcgac agtaatcctt gctcatacca ttaaaggtta cggcatgggc
     2281 gacgcggctg aaggtaaaaa catcgcgcac caggttaaga aaatgaacat ggacggcgtg
     2341 cgtcacatcc gcgaccgttt caatgtgccg gtgtctgatg cagatatcga aaaactgccg
     2401 tacatcacct tcccggaagg ttctgaagag catacctatc tgcacgcaca gcgtcagaaa
     2461 ctgcacggtt atctgccaag ccgtcagccg aacttcaccg agaagcttga gctgccgagc
     2521 ctgcaagact tcggcgcgct gctggaagag cagagcaaag agatctctac cactatcgct
     2581 ttcgttcgtg ccctgaacgt aatgctgaag aacaagtcga tcaaagatcg tctggtaccg
     2641 atcatcgccg acgaagcgcg tactttcggt atggaaggtc tgttccgtca gattggtatt
     2701 tacagcccga acggtcagca gtacatcccg caggaccgcg agcaggttgc ttactataaa
     2761 gaagacgaga aaggtcagat tctgcaggaa gggatcaacg agctgggcgc agggtgttcc
     2821 tggctggcag cggcgacctc ttacagcacc aacaatctgc cgatgattcc gttctacatc
     2881 tattactcga tgttcggctt ccagcgtatc ggcgatctgt gctgggcggc tggcgaccag
     2941 caagcgcgtg gcttcctgat cggcggtact tccggtcgta ccaccctgaa cggtgaaggt
     3001 ctgcagcacg aagatggtca cagccacatt cagtcgctga ctatcccgaa ctgtatctct
     3061 tacgacccgg cttacgctta cgaagttgct gtcatcatgc atgacggtct ggagcgtatg
     3121 tacggtgaaa aacaagagaa cgtttactac tacatcacta cgctgaacga aaactaccac
     3181 atgccggcaa tgccggaagg tgctgaggaa ggtatccgta aaggtatcta caaactcgaa
     3241 accattgaag gtagcaaagg taaagttcag ctgctgggct ccggttctat cctgcgtcac
     3301 gtccgtgaag cggcagagat cctggcgaaa gattacggcg taggttctga cgtttatagc
     3361 gtgacctcct tcaccgagct ggcgcgtgat ggtcaggatt gtgaacgctg gaacatgctt
     3421 catccgctgg aaactccgcg cgttccgtat atcgctcagg tgatgaacga cgctccggca
     3481 gtggcatcta ccgactatat gaaactgttc gctgagcagg tccgtactta cgtaccggct
     3541 gacgactacc gcgtactggg tactgatggc ttcggtcgtt ccgacagccg tgagaacctg
     3601 cgtcaccact tcgaagttga tgcttcttat gtcgtggttg cggcgctggg cgaactggct
     3661 aaacgtggcg aaatcgataa gaaagtggtt gctgacgcaa tcgccaaatt caacatcgat
     3721 gcagataaag ttaacccgcg tctggcgtaa gaggtaaaag aataatggct atcgaaatca
     3781 aagtaccgga catcggggct gatgaagttg aaatcaccga gatcctggtc aaagtgggcg
     3841 acaaagttga agccgaacag tcgctgatca ccgtagaagg cgacaaagcc tctatggaag
     3901 ttccgtctcc gcaggcgggt atcgttaaag agatcaaagt ctctgttggc gataaaaccc
     3961 agaccggcgc actgattatg attttcgatt ccgccgacgg tgcagcagac gctgcacctg
     4021 ctcaggcaga agagaagaaa gaagcagctc cggcagcagc acccgcggct gcggcggcaa
     4081 aagacgttaa cgttccggat atcggcagcg acgaagttga agtgaccgaa atcctggtga
     4141 aagttggcga taaagttgaa gctgaacagt cgctgatcac cgtagaaggc gataaagctt
     4201 ctatggaagt tccggctccg tttgctggca ccgtgaaaga gatcaaagtg aacgtgggtg
     4261 acaaagtgtc taccggctcg ctgattatgg tcttcgaagt cgcgggtgaa gcaggcgcgg
     4321 cagctccggc ggctaaacag gaagcagctc cggcagcggc ccctgcatca gcggctggcg
     4381 tgaaagaagt taacgttccg gatatcggcg gtgacgaagt tgaagtgacc gaagtgatgg
     4441 tgaaagtggg cgacaaagtt gccgctgaac aatcgcttat caccgtagaa ggcgataaag
     4501 cttctatgga ggtcccggct ccgtttgcag gcgtcgtgaa ggaactgaaa gtcaacgttg
     4561 gcgataaagt gaaaactggc tcgctgatta tgatcttcga agttgaaggc gcagcgcctg
     4621 cggcagctcc tgcgaaacag gaagcggcag cgccggctcc ggcagcgaaa gctgaagccc
     4681 cggcagcagc tccggctgcg aaagccgaag gcaaatctga atttgctgaa aacgacgctt
     4741 acgttcacgc gactccgctg atccgccgtc tggcacgcga gtttggcgtt aacctggcga
     4801 aagtgaaggg cactggtcgt aaaggtcgta tcctgcgcga agacgttcag gcttacgtga
     4861 aagaagctat caaacgtgca gaagcagctc cggcggcgac tggcggtggt atccctggca
     4921 tgctgccgtg gccgaaggtg gacttcagca agtttggcga aatcgaagaa gtggaactgg
     4981 gccgtatcca gaaaatttct ggtgctaacc tgagccgtaa ctgggtgatg atcccgcatg
     5041 ttacccactt cgacaaaacc gatatcaccg agctggaagc gttccgtaaa cagcagaacg
     5101 aagaagcggc gaaacgtaag ctggatgtga agctcacccc ggttgtcttc atcatgaaag
     5161 ccgttgctgc agctcttgag cagatgcctc gcttcaatag ttcgctgtcg gaagacggtc
     5221 agcgtctgac cctgaagaaa tacatcaaca tcggtgtggc ggtggatacc ccgaacggtc
     5281 tggttgttcc ggtattcaaa gacgtcaaca agaaaggcat catcgagctg tctcgcgagc
     5341 tgatgactat ttctaagaaa gcgcgtgacg gtaagctgac tgcgggcgaa atgcagggcg
     5401 gttgcttcac catctccagc atcggcggcc tgggtactac ccacttcgcg ccgattgtga
     5461 acgcgccgga agtggctatc ctcggcgttt ccaagtccgc gatggagccg gtgtggaatg
     5521 gtaaagagtt cgtgccgcgt ctgatgctgc cgatttctct ctccttcgac caccgcgtga
     5581 tcgacggtgc tgatggtgcc cgtttcatta ccatca
//
LOCUS       DAZPAC010000251         5614 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZPAC010000251 DAZPAC010000000
VERSION     DAZPAC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.252"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..428
                     /gene="soxR"
                     /locus_tag="ACHUPM_003877"
     CDS             <1..428
                     /gene="soxR"
                     /locus_tag="ACHUPM_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR0871534.1"
                     /translation="PGEVAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYV
                     AIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDE
                     LDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="ACHUPM_003878"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="ACHUPM_003878"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR0871535.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="ACHUPM_003879"
     CDS             2475..4124
                     /locus_tag="ACHUPM_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR0871536.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5569)
                     /locus_tag="ACHUPM_003880"
                     /pseudo
     CDS             complement(4278..5569)
                     /locus_tag="ACHUPM_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1256 a         1270 c         1456 g         1632 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc tctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt agagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg acattattta taaaatatac caccttaatg gatacttcct
     5221 tttctttact aaggtaaact cttctcgtaa ttctatcgtc acatttatta tttgcatata
     5281 aagctatatc tataaaatgt ttatgtattt tcctgtccgt ttccttcgtg atttttcctg
     5341 atggtaaact atcataaatt cttttcattt tctgaatcaa acctatcact ttttcatgat
     5401 catccatctt gaaaagagag agggctttat tgtcaattaa cgtgttttta tgatgtttgc
     5461 tgtaattata tgattttgtt ttctttgtat gtgatgaact tgtgaaagag tggttatcgt
     5521 taatctggca aagagagaaa aacatattat ttaaaccatt atagcgcata aaatatcctt
     5581 ataattaaca agaaagaaaa ggcatattct ctgc
//
LOCUS       DAZPAC010000252         5584 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZPAC010000252 DAZPAC010000000
VERSION     DAZPAC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.253"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..116)
                     /locus_tag="ACHUPM_003881"
     CDS             complement(<1..116)
                     /locus_tag="ACHUPM_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR0871537.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
     gene            complement(221..1723)
                     /gene="dtpA"
                     /locus_tag="ACHUPM_003882"
     CDS             complement(221..1723)
                     /gene="dtpA"
                     /locus_tag="ACHUPM_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR0871538.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2334..2969)
                     /gene="nth"
                     /locus_tag="ACHUPM_003883"
     CDS             complement(2334..2969)
                     /gene="nth"
                     /locus_tag="ACHUPM_003883"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR0871539.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(2969..3664)
                     /gene="rsxE"
                     /locus_tag="ACHUPM_003884"
     CDS             complement(2969..3664)
                     /gene="rsxE"
                     /locus_tag="ACHUPM_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR0871540.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3668..4288)
                     /gene="rsxG"
                     /locus_tag="ACHUPM_003885"
     CDS             complement(3668..4288)
                     /gene="rsxG"
                     /locus_tag="ACHUPM_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR0871541.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4292..5350)
                     /gene="rsxD"
                     /locus_tag="ACHUPM_003886"
     CDS             complement(4292..5350)
                     /gene="rsxD"
                     /locus_tag="ACHUPM_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR0871542.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(5351..>5584)
                     /locus_tag="ACHUPM_003887"
     CDS             complement(5351..>5584)
                     /locus_tag="ACHUPM_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871543.1"
                     /translation="ARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAE
                     PEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         1457 a         1419 c         1376 g         1332 t
ORIGIN      
        1 ttttcgagac gttttttcat taaatccaca ctgacgaggg taaaatcctt tccgctctca
       61 cgcagggtga tatgggaagc gagagagcag gcacccggtt tgtagaacaa tttcatcggt
      121 aactcctttt gtctgtggtt taaagtatgg tagtgcgttg ctgaacaaaa aaaagccgct
      181 aacgaaaagt tagcggcttg taaaagagtt tccctgaatt ttacgctaca gcagctttcg
      241 ccgctttgtc tgcagcgtca tcctgcgtca tacggtgcag tttaggcgcg gtcagcagca
      301 tcagtactgc aataatggca gtagcgacac caatctgcaa gaatacgcga ccataaactt
      361 ccagtgacat cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac
      421 caccaatcag gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca
      481 gacgctgcgg aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc
      541 cgatgctctg caggccatag cttgcgacca gccagcttac agacacgata ccagcgtcag
      601 acgcgaattt cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga
      661 ttgcaaactt ggttggcatc ggcagggtat cgcccatctt gttatagata gcggccagaa
      721 tcggactacc gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg
      781 ccagacccag aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca
      841 tctggctgta cagcacgaag aagataatgg cttcgagcat caggatgaag gcaacgatca
      901 ttttacgacg cgcagcacct ttcatcgcga aggcttcttt accgaagata accacgatac
      961 cgaaggcaac aacgcccagc gccatacgcg caacttcctg attgtgcagc agccaggtgg
     1021 cgatagcgat cagtgccaca acaccaataa tggtcagcag caggttacgg tagttgatag
     1081 gctcgaagtc tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa
     1141 cgatagtgat cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg
     1201 cggccagcca cggcgtagca atcatagaga agaaagagcc gatgttgacg gacatgtagt
     1261 acatggtgaa tgcaccgtcc agacgcgggt cgtttttctc atagcatgta gaaagcagag
     1321 aagatgggtt agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa
     1381 cgataccggc gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag
     1441 caccgagcat aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag
     1501 cgaccagacc ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag
     1561 acatacccag ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa
     1621 aacgttccca taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt
     1681 tcaaactgac gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta
     1741 catcccatat taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt
     1801 tatagtggga ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt
     1861 tgtaataatc tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc
     1921 cagcgttaaa tttttcaaaa aacgtaatgg cagatttttt cactgcactg agaggggggt
     1981 gatggcttaa cggcgatatg ttctgctatt tagcgttttt actagtgaga tagtccagtt
     2041 tctgaaaaat agccagtgta atgttttgta ggtcaatatt tgttgcgtgt gtaaatttaa
     2101 attattttaa cctgattctt gcaatgtttt tacattgcga cagcatcgtg atcttgatca
     2161 cgaatgaata gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc
     2221 aaacggatta cctgatgata acgctaagca atggcatcat tcacgttaaa aagatgtttt
     2281 aaaagaggat aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg
     2341 tcaactttct ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg
     2401 cgggcaatgc aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac
     2461 tctgccggaa ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca
     2521 aattgagtac gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg
     2581 aatgcagtgt ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc
     2641 gcagcacgat cttccggaac ctcgccatta tgctgctcca gtaagatacg gcaggttttg
     2701 atgatatttt ctgctttgct gttataaagc ccaatcgttt tgatataggt tttaacccct
     2761 tcaacgccca gttcaagcat cgctgcaggc gtattcgcca ccgggtaaag tttcgccgtc
     2821 gccttattaa cactgacatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca
     2881 aaaggcgaac tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg
     2941 aggatctcca ggcgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg
     3001 cacgttcggc agctgcttca gtacggcgct ttttcattct ttcatcaatc aggtattttc
     3061 ctgccagcat cagtcccagg ccaataaatg cacctggtgg cagcatcgcc agcaggaaag
     3121 gggagtcggt gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat
     3181 ctgcaccgtc aaacaatgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca
     3241 tggcgcaggt tgcgcccata ccaattgaaa agccgtccag tgccgaaagc gccggacctt
     3301 ttttggcggc gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa
     3361 taaaaatccc taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag
     3421 cgctgaccac cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc
     3481 agtgacgcag cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc
     3541 ctaaacccag agcgttagtg gcagtggacg tgaccgccaa cagaggacaa aggccgagca
     3601 actggaccag cgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc
     3661 tcacgcttta ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt
     3721 acaatccggc gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga
     3781 actggtcgaa atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga
     3841 tttttttacc cgcaaaatgg gtgatccagt cagaaaggcg cagttcgatt ttatcgccaa
     3901 gccctggcgt ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgccg ttaaaatcgg
     3961 ctcccaccag cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg
     4021 cggctaccgg tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact
     4081 ctggcgcagt taccagatag caactctgtg ccagcgtatt gttatagcgt tcggctggca
     4141 gcacctgatc aaataatgcc ttttgttgca gactggcctg ttcagcaatc gtcgttttgg
     4201 tcatctggtt gatggccgca gttaaccctg ttgaacccgc tgcaaatagc gctaacgtaa
     4261 tgccgtgttt tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg
     4321 gacgcgtgta gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca
     4381 cgccgtcagg atagccgccg aaactgcgga tcaaccagac taataagccc gcaagcgcgc
     4441 cgaaaataag acgaccacga ttggtcgtag aagcggtaac cgggtcagtc aaaataaaga
     4501 atgcgccgag catggtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg
     4561 gtgagaacaa ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa
     4621 tatgccagcg aatcgctttc tgccatagca accacaggcc gccagccagc caggcgagat
     4681 ttacccattg ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct
     4741 gttcaaccga atgaccggca cggacagagg ttttaaatgt atccagcggt gtcgcctgac
     4801 taatgccatc aatacctaag cgtagtgtgt tcatatcacc accactggcg gtatgcccgc
     4861 tgaaaataac ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg
     4921 gtaaccagct ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg
     4981 ccggattaaa cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa
     5041 acaccgtacc cagcacgacc atccaccatg gcgcgagggg gggaatactt accgccagca
     5101 ataagcctgt cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta
     5161 gtttgagtac gagagcttcg gctaacagag tactaaccga tgccaacagg atctgaacga
     5221 gagtacccca accaaaaaac cacagttgcg ctgcgattcc tggcacggct gcgagcaaca
     5281 ccaacagcat aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc
     5341 tgaataccat ttagtcctcg tttacaacct tctgctgggc ggcttttttg gcctgagcgc
     5401 gggcaatagc cgcggcaact gccgctttgc gcggatcaac ctgttcttct ggttctgcgt
     5461 tagtctgttg ctgttccagc ttgcgcgctt tggcacgggc aatagcggct tcgacggcgg
     5521 ctttgcgcgg atcaacctgt tcttctggtt ccgcattagc ctgttgctgt tccagtttgc
     5581 gcgc
//
LOCUS       DAZPAC010000253         5542 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZPAC010000253 DAZPAC010000000
VERSION     DAZPAC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.255"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(449..3991)
                     /locus_tag="ACHUPM_003888"
                     /pseudo
     CDS             complement(449..3991)
                     /locus_tag="ACHUPM_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4059..>5542)
                     /gene="tssI"
                     /locus_tag="ACHUPM_003889"
     CDS             complement(4059..>5542)
                     /gene="tssI"
                     /locus_tag="ACHUPM_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR0871544.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIE
                     TVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRV
                     GLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGT
                     KIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
BASE COUNT          965 a         1821 c         1462 g         1294 t
ORIGIN      
        1 accccagcgt cccaagctct tcaaaaaact tcattggaga atctttgata ttctcaatct
       61 tttcaccata aacgtctaat attgatggtt caccagggtc atcaaactgc tcaccataag
      121 gtaaaatacc caataaattt ctccaacacc tatctctacc attttcctga agtgattctg
      181 ataattgtaa atttgctctg ttatatgcag ctgtcgcaga ttttatattc cacgccccta
      241 aagccataga agctgcaccg actggggcag caatgatcgt tccatcggca gcaacactta
      301 taccgaattt tgtcatgcca cttgcataca gccttgctga attagcagta tttaaactag
      361 ctgtaaacat ttttgtcggg ttaaacggaa ggttatccag tttattattt atatcatcta
      421 taaaatgact acaactctca catgagaata accccaacgg atctatcctg ctcaccggat
      481 tcagcggata ctgatacaga ttccacccac ctttcagccc aatggggtcc tgggtgatgt
      541 accgtctctg caacggatca tagtaccggt gacgattgta gtacagcccc gactcctcat
      601 catactgctg ccccggcaga cggtatggct gatacacatg atgcgggttc tcctcattta
      661 gctggttgcc ccactcatca tacgccccgc tccacaccgt atcgccgtct tcgctgatga
      721 gcgccagcgg ctggccccga tggtcgcagt ggtaaagatg aacgtttcgc actggtgtgt
      781 attccggctc cacctgtctg gccagttgct ccaccgtcag cccgcactgc gcaagccaca
      841 cccggctttc actgctcacg cggtctgccc ggatttcttc ctccagtctg tccagcagcc
      901 gcaccagttc agccggaaat accacgccgt gcccgtcctc gctcccttcc tgctgcagct
      961 tctccgccag gctgcggcgc tgcgctttct cccgctcgcc gttctctgtc tcgacccgga
     1021 tgagcggcgt gaagcttccc ggctcgtata ccgtctggat acgtgtggtg tcggtctgta
     1081 ccgtcgtcag cctgtcgccg tcccagccgt accatgtctc ctccggttta cgcgacagcg
     1141 acatccaccc cgtcaggtca cgctcccgcc gccatacccg tttcgccatt cgccgtccca
     1201 gcgggtcgta gaggtagcgg ctctcgacca gtggctcgcc atgctgtatc cgcgtgtgga
     1261 acaccaggcg gtgctggctg tcgtagtggt gggtccgctc gtcgtccgtc cgtatcacac
     1321 ccgtcgggat gcggtccgtc ttctccgtca gtctgccgta ttcatcatag tggtaaacat
     1381 agtgcgcatc ctctgcgatg cggttatccg gccacgccgt gagcgtgctg tccgggtgta
     1441 gctccgggtc cggcaggcgg tttcccgccg ggtcggtggc atacgggatg cggatgtcca
     1501 ggtccggtgc gagggtgcgc accgtctcac ggtgcagcct gtcccgcgta tactcgacca
     1561 gcggcgtccc gcccagcttc atgcccgcca ggtaaccgct gccgtaggtc agccactcca
     1621 ccggcggcag gctgtccggc gtgacgcggt ttgccagccc ctgttcgttg taggcgtgtt
     1681 tcgtctcatg ctgccacagc agttcccccg tctccgggtt ctccaccgtc tgccgttcgc
     1741 ccgtcaggcg gcccttatcg tcgtagccgt agtggacagc gacacggtgg ccttcgctca
     1801 ggtggctgat ttctctcagc cagccgtggc cgtcatactg ccactgctct gccgggtcgc
     1861 cgttcaccgt ccggtgcgtg atgcggtccg acgcatcgta gtgccagagg gtgacaagcc
     1921 cctcatcctc actctgcgtg agttttccgg tcaggtcata atgataacgt tgcgtccgcc
     1981 cgtcaaagcc gccctgctgt accagccggt ccagcgcatc gtagctgaag tcgctgtgac
     2041 tgccgttctc gttagtcaga ctgatgacac gtccggcagc atcgtactcc atactgcgcg
     2101 tcagcccgcc ctgcgtggtg ctgaccgcct ttccccacgc atcgtactgt gtctcgctcc
     2161 ggttgccgtc cggggtgata acggcagtca ggtcgcctgc ggcgttgtac tcatactgcg
     2221 tttcacggcc ctgtgcgtct ttcaccgagg ttaaccggcc acggttgtca tagtggcggt
     2281 aaaggctgat accttcctcg cggtgaaccg ccgtcatctg gccgaagcgg tcgtattcat
     2341 agcgggtctg gtagcccgag cagtcagtga acgccagcaa ctgcccgtag cggctccacg
     2401 tcatctgccg ggtgctgccc gtcgcatccg ttgtcgtcgc cggtaactca ctgtgcgcgt
     2461 catcgtagcg gtagcgtact gtctccccgc tgcgcgatgt ctccgatacc agcctgcccg
     2521 gttcatcata ttcccggcgg ctctccagcc cgtccgggta caccaccgcc gtcagctggt
     2581 tcccgtcgtt atagtaaaat ttcgtctccc gcccgtccgg tgtggtgatg tccgtgatat
     2641 cgccggacac cacattcaga ccgtactctg tccgccgtcc cgccgcgtcc gtctgcgccg
     2701 tgagccttcc tgccgcgtca tacccgctgc gcgtgacgct gccgtccgcc agttcttttt
     2761 tcaccacccg tttcagcccg gccccgcctt ctgtatgcag cacctcacgc cggttcaggc
     2821 tgtcggtgac ggtgatgcgg tcctgctcat aaagatagcg gtagcttaac cctgccgggt
     2881 tcagttgctc caccacccgc cccgtatcgt cgtagcggta gcgcatctcc ggccttcccg
     2941 cgtaacggtg cgccaccatc cggcccgggc gctgcgcgtc atacgtgaaa gcgcgcacct
     3001 gcgtattgct gcggtcatat accgccagca gttcaccggc ttccgtatac gtgtaccgca
     3061 ccagtggcgc agcgggcagg ctctccgggt atgccgggtc gtgcatcaac cacaccgccg
     3121 aaaggcggat acccctgtcg gggccgtatt cggtaccggg cagtgtgtcg gggaacgctg
     3181 aggctgagag agggcgggaa ctgtcagaag aagatagcga agaggtgcgg gcctcttccg
     3241 cacgctgcgc ctgcgtggtc agcaccagac ggaactcccg cccggcaccg tccgtcacgt
     3301 cggtgatttc cccggccagg tcaccggcgg cctcacgccg gtacgtcagc gtccgccatc
     3361 ccggtaagca cccggtacgg cggcagcggc gctggcagta cgtcctcagc acccggcacc
     3421 cgctcagccc cccccagtat ccaccacggc ccctgtgcgc tgttggtcgc caggtaaaga
     3481 tgcgggctta accggatatc cggcgcagcg ccccccacag ccgcgccagc gtatggccgt
     3541 ccggctgtgc tgccttacca ccgcgcacca gccacattga ctcactgcgg ctgtacaccg
     3601 cctcccccgg cagcagcggc tcaaagtgaa tgctccgccc gccgttgtcg ttgagtatca
     3661 gtccgtcatc acgtagctgt aagcggatat cagaaggcgc tttccagccg gggccgaaaa
     3721 cgcccaccgg tgcaggcgtc ttcgtccggt agctgctgta ggtgcgggag agaatgaacg
     3781 gcagcgggcc gggcagcgca aggtccgtct cgccgggcag caccttcgcc cccagcagcg
     3841 gatttaccgg gttgcccgaa gtcatcccgc ccggacacac cgagcacgcc acgccggtgg
     3901 gcgcgccaat tcttacacct gccgaaccct ggacaatggg accgccatac tgagtcatat
     3961 ctccctgacg cgccgctggt tttccgctca tgattcaaat tccttcttct gtttattttt
     4021 aatgctggtt tgttactgaa taccgaacag gtacgggatc agtactctct catatccggc
     4081 gtatcctgct tttcatcagg ggtcttcggt ggggatttcg aggctgcaca gttccagtta
     4141 atcaacagtg agtcaccatt aacctgctcc ttaccctgca aatgaatttt tgtgccgttg
     4201 agaaaaattt gtccgtcctt cgtcagcacc agccttgcct taccacagca gagctccaga
     4261 tgctcaccgg cggagtaaat cgcggtctgc ccggtatcat cctttttcgt tttaccaacg
     4321 gtgaaggtaa cgttatttcc gactttgacg tcataaccca gcccgaccct caacgattta
     4381 tgcaaaccga tctgcgagga ctgcatcagt gccaccgagg tgttcataat gccacctacc
     4441 gtcgtctgat acgccacgcc caccgtcagc gcacgaacaa gtccaatcgt ttccacctga
     4501 acagaaccca ctttaattat ctggttactg cccaccgtct ctatctggtt aacgcccacc
     4561 gtctctatct gattgttggt aaccgcaatc atctgattgt tgccaatggt ttcagcatgg
     4621 ttgtttatca catccgtggt gcggttattc agcacctcgg tgttcatgtt cttctgcgcg
     4681 tggatgtaga cctgttcttt ccctgtcgca tcgtcaaact tcagttcatt aaacccgctg
     4741 cccttatagg ttttcgaacg aatggtcatc tgcgtcttcg tccccggcag gctgccgggt
     4801 gtgcggtttt cctggtggta ggtacgcccc ataataatcg gctggtccgg gtcgccgttg
     4861 aggaagtcca caatcacctc ctgacccaca cgcggtatcg ccaggttgcc aaagccggtg
     4921 cctgcccacg cctgtgccac acggatccag catgaactgt cctggtttga cgggttatag
     4981 cggtcccagt taaatttcac ccgcacgcgg ccatgttcat cacagaagat ttcctcgcct
     5041 gccggtcccg tcacgacggc gctctgcggg ccatccacca gcggtttcat caacggctgt
     5101 ggccgccatg ttctgtctgc cgggattacc gcaaaatggt tctccagcgt ggtgcctgaa
     5161 cccctgcgtc ccggtaccgc ctgcggctgt tcgccgtgaa gatcgctcgc caccacctgc
     5221 cattcccggt tcaggttcgc ctgcggatgc cccgtcagca caattcgccg tcccggccat
     5281 atctccggcg aacggcttgt tccgcgcgcc acttctgcgt tgtttcgcca gccgtccatc
     5341 tgccagcggg caaagttctg cccgtgggca cccttgaaac gtccggggta gtcatacact
     5401 tcatactgcg tgcgctggta atcctggtac tggccttcct gatcaaaacg ccctggccag
     5461 ccgggacgtt taaaggtgta gtctttggtc actacggaag aaggacggat ttgtgcgctg
     5521 tagcgaaact ggctgatgca ga
//
LOCUS       DAZPAC010000254         5527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZPAC010000254 DAZPAC010000000
VERSION     DAZPAC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.256"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="ACHUPM_003890"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="ACHUPM_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR0871545.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="ACHUPM_003891"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="ACHUPM_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFR0871546.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="ACHUPM_003892"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="ACHUPM_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR0871547.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHUPM_003893"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHUPM_003893"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR0871548.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHUPM_003894"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHUPM_003894"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR0871549.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="ACHUPM_003895"
     CDS             4447..4812
                     /locus_tag="ACHUPM_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR0871550.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="ACHUPM_003896"
     CDS             4962..5480
                     /locus_tag="ACHUPM_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR0871551.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1520 a         1391 c         1240 g         1376 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatatttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgca
//
LOCUS       DAZPAC010000255         5457 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZPAC010000255 DAZPAC010000000
VERSION     DAZPAC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.257"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            302..1159
                     /gene="focA"
                     /locus_tag="ACHUPM_003897"
     CDS             302..1159
                     /gene="focA"
                     /locus_tag="ACHUPM_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR0871552.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1214..3496
                     /gene="pflB"
                     /locus_tag="ACHUPM_003898"
     CDS             1214..3496
                     /gene="pflB"
                     /locus_tag="ACHUPM_003898"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR0871553.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3688..4428
                     /gene="pflA"
                     /locus_tag="ACHUPM_003899"
     CDS             3688..4428
                     /gene="pflA"
                     /locus_tag="ACHUPM_003899"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR0871554.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4560..4697)
                     /locus_tag="ACHUPM_003900"
                     /pseudo
     CDS             complement(4560..4697)
                     /locus_tag="ACHUPM_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            4797..>5457
                     /locus_tag="ACHUPM_003901"
     CDS             4797..>5457
                     /locus_tag="ACHUPM_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0871555.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
BASE COUNT         1410 a         1325 c         1299 g         1423 t
ORIGIN      
        1 tgtaattata aggttaaata tcggtaattt gtatttaata aatacgatcg atattgttac
       61 tttattcgcc tgatgctccc ttttaattaa ctgttttagc ggaggatgcg gaaaaaattc
      121 aactcatttg ttaattttta aaatttattt ttatttggat aatcaaatgt ttactccgta
      181 tctgcataaa aaccatgcga gttacgggcc tataagccag gcgagatatg atctatatca
      241 atttctcatc tataatgctt tgttagtatc tcgtcgccga cttaataaag agagagttag
      301 tgtgaaagct gacaaccctt ttgatctttt acttcctgct gcaatggcca aagtggccga
      361 agaggcgggt gtctataaag caacgaaaca tccgcttaag actttctatc tggcgattac
      421 cgccggtgtt ttcatctcaa tcgcattcgt cttctatatc acagcaacca ctggcacagg
      481 cacaatgccc ttcggcatgg caaaactggt tggcggcatt tgcttctctc tggggctgat
      541 tctttgtgtt gtctgcggag ccgatctctt tacttccacc gtgttgattg ttgttgctaa
      601 ggcgagtggg cgcatcacct ggggtcagtt ggcgaaaaac tggctaaatg tctattttgg
      661 caacctggtc ggcgcactgc tgtttgtact tttaatgtgg ctttccggcg agtatatgac
      721 cgcaaatggt caatggggac taaacgtcct acaaaccgcc gaccacaaag tgcaccatac
      781 ttttattgag gccgtctgtc ttggtatcct ggcaaacctg atggtatgtc tggcagtatg
      841 gatgagttat tctggccgca gcctgatgga caaagcgttc attatggtgc tgccggtcgc
      901 gatgtttgtt gccagcggtt ttgagcacag tatcgcaaac atgtttatga tcccgatggg
      961 tattgtaatc cgcgacttcg catccccgga attttggacc gcagtcggtt ctgcaccgga
     1021 aaatttttct cacctgaccg tgatgaattt catcactgat aacctgattc cggttacgat
     1081 cggcaacatt atcggtggtg gtttgttggt tgggttgaca tactgggtca tttacctgcg
     1141 tgaaaacgac caccattaat ggttgtcgaa gtacgcagta aataaaaaat ccacttaaga
     1201 aggtaggtgt tacatgtccg agcttaatga aaagttagcc acagcctggg aaggttttac
     1261 caaaggtgac tggcagaatg aagtaaacgt ccgtgacttc attcagaaaa actacactcc
     1321 gtacgagggt gacgagtcct tcctggctgg cgctactgaa gcgaccacca ccctgtggga
     1381 caaagtaatg gaaggcgtta aactggaaaa ccgcactcac gcgccagttg actttgacac
     1441 cgctgttgct tccaccatca cctctcacga cgctggctac atcaacaagc agcttgagaa
     1501 aatcgttggt ctgcagactg aagctccgct gaaacgtgct cttatcccgt tcggtggtat
     1561 caaaatgatc gaaggttcct gcaaagcgta caaccgcgaa ctggatccga tgatcaaaaa
     1621 aatcttcact gaataccgta aaactcacaa ccagggcgtg ttcgacgttt acactccgga
     1681 catcctgcgt tgccgtaaat ctggtgttct gaccggtctg ccagatgcat atggccgtgg
     1741 ccgtatcatc ggtgactacc gtcgcgttgc gctgtacggt atcgactacc tgatgaaaga
     1801 caaactggca cagttcactt ctctgcaggc tgatctggaa aacggcgtaa acttggaaca
     1861 gactatccgt ctgcgcgaag aaatcgctga acagcaccgc gctctgggtc agatgaaaga
     1921 aatggctgcg aaatacggct acgacatctc tggtccggct accaacgctc aggaagctat
     1981 ccagtggact tacttcggct acctggctgc tgttaagtct cagaacggtg ctgcaatgtc
     2041 cttcggtcgt acctccacct tcctggatgt gtacatcgaa cgtgacctga aagctggcaa
     2101 gatcaccgaa caagaagcgc aggaaatggt tgaccacctg gtcatgaaac tgcgtatggt
     2161 tcgcttcctg cgtactccgg aatacgatga actgttctct ggcgacccga tctgggcaac
     2221 cgaatctatc ggtggtatgg gcctcgacgg tcgtaccctg gttaccaaaa acagcttccg
     2281 tttcctgaac accctgtaca ccatgggtcc gtctccggaa ccgaacatga ccattctgtg
     2341 gtctgaaaaa ctgccgctga acttcaagaa attcgccgct aaagtgtcca tcgacacctc
     2401 ttctctgcag tatgagaacg atgacctgat gcgtccggac ttcaacaacg atgactacgc
     2461 tattgcttgc tgcgtaagcc cgatgatcgt tggtaaacaa atgcagttct tcggtgcgcg
     2521 tgcaaacctg gcgaaaacca tgctgtacgc aatcaacggc ggcgttgacg aaaaactgaa
     2581 aatgcaggtt ggtccgaagt ctgaaccgat caaaggcgat gtcctgaact atgatgaagt
     2641 gatggagcgc atggatcact tcatggactg gctggctaaa cagtacatca ctgcactaaa
     2701 catcatccac tacatgcacg acaagtacag ctacgaagcc tctctgatgg cgctgcacga
     2761 ccgtgacgtt atccgcacca tggcgtgtgg tatcgctggt ctgtccgttg ctgctgactc
     2821 cctgtctgca atcaaatatg cgaaagttaa accgattcgt gacgaagacg gtctggctat
     2881 cgacttcgaa atcgaaggcg aatacccgca gtttggtaac aacgatccgc gtgtagatga
     2941 cctggctgtt gacctggtag aacgtttcat gaagaaaatt cagaaactgc acacctaccg
     3001 tgacgctatc ccgactcagt ctgttctgac catcacttct aacgttgtgt atggtaagaa
     3061 aacgggtaac accccagacg gtcgtcgtgc tggcgcgccg ttcggaccgg gtgctaaccc
     3121 gatgcacggt cgtgaccaga aaggtgcagt agcctctctg acttccgttg ctaaactgcc
     3181 gtttgcttac gctaaagatg gtatctccta caccttctct atcgttccga acgcactggg
     3241 taaagacgac gaagttcgta agaccaacct ggctggtctg atggatggtt acttccacca
     3301 cgaagcatcc atcgaaggtg gtcagcacct gaacgttaac gtgatgaacc gtgaaatgct
     3361 gctcgacgcg atggaaaacc cggaaaaata tccgcagctg accatccgtg tatctggcta
     3421 cgcagtacgt ttcaactcgc tgactaaaga acagcagcag gacgttatta ctcgtacctt
     3481 cactcaatct atgtaattag atttgactga aatcgtacag taaaaagcgt acaataaagg
     3541 ctccacgaaa gtggggcctt ttttagcacg agagcctttt ttgtcagcta tctatactct
     3601 aaggtgactg ccaaaacaga ctcgacgtag ccttcgagct gcgcaccaac acggcctcag
     3661 atgggccaca tctggagaaa caccgcaatg tcagttattg gtcgcattca ctcctttgaa
     3721 tcctgtggaa ccgtagacgg cccgggtatt cgctttatca cctttttcca gggctgcctg
     3781 atgcgctgcc tgtattgtca taaccgcgac acctgggata cgcatggcgg taaagaagtt
     3841 accgttgaag atttaatgaa agaggtggtg acctatcgcc actttatgaa cgcttccggc
     3901 ggcggcgtta ccgcatccgg cggtgaggca atcctacaag ctgagtttgt tcgtgactgg
     3961 ttccgcgcct gcaaaaaaga aggcattcat acctgtctgg acaccaacgg ttttgttcgt
     4021 cgttacgatc cggtgattga tgaactgctg gaagtaaccg acctggtaat gctcgatctc
     4081 aaacagatga acgacgagat ccaccaaaat ctggttggag tttccaatca ccgcacgctg
     4141 gagttcgcca aatatctggc gaacaaaaat gtgaaggtgt ggatccgcta cgttgttgtc
     4201 ccaggctggt ctgacgatga cgattcagcg catcgcctcg gtgaatttac ccgtgatatg
     4261 ggcaacgttg agaaaatcga gcttctcccc taccacgagc tgggcaaaca caaatgggtg
     4321 gcaatgggtg aagagtacaa actcgacggt gttaaaccac cgaagaaaga gaccatggaa
     4381 cgcgtgaaag gcattcttga gcagtacggt cataaggtaa tgttctaatt tccccgtaaa
     4441 gcggcaactt tattgagttg ccgctttttt attccgcact tcattctgct acagcgtttt
     4501 taacacatct tatagtgcat ctaccatatc ccgtacctga gataataacg actgataata
     4561 ttacgccgat ataaatcgag ttccgtcacc tgtattttcc gctccattgc ccgctgatgg
     4621 atcgcatcag caatatgtgc ggtcaatgaa tcatgacgag gatgggccca taccaaataa
     4681 atacgttcag actgcataat tttacctcca cgttaagttc tggagcgcac tttacgcagg
     4741 cggcataata atgtaaacgt gatgattcag gatgagttaa tgaaggattt tcactaatgc
     4801 ggatgaatat gtctgacttt gccactttct ttgctgtggc ccgtaatcaa agctttcgtg
     4861 cagcgggcga tgagttaggc ttatcctcgt ccgccattag ccatagtatt aaaacactgg
     4921 aacaacgtct taaaattcgc ttattcaatc ggacaacccg aagcgtatcg ttaacggaag
     4981 cgggttctaa tctctatgaa cgattgcgcc cagctttcga cgaaattcag atcatgttag
     5041 atgagatgaa tgattttcgt ctgacaccaa caggaacgct caaaatcaat gccgcccggg
     5101 tagctgcacg tatattttta atgcctttac tggttggttt cacacgtgaa tatcctgata
     5161 ttaaagtcga gctcacaact gacgactctc ttgtcgatat cgtccaacag ggctttgacg
     5221 ctggcgttcg acttagtggc atcgtcgaaa aagatatgat cagcgtggcg atcggcccac
     5281 cagtgaaatt atgtgtagca gcaacgccag agtactttgc aagatatggc aaaccccgcc
     5341 atcctcatga tctgcttaat catcaatgtg ttgtatttcg ttatcccagc ggcaaacctt
     5401 tccactggca atttgcaaag gagcaggaaa ttgctgtcgc agggaatatc attctgg
//
LOCUS       DAZPAC010000256         5341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZPAC010000256 DAZPAC010000000
VERSION     DAZPAC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.258"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUPM_003902"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUPM_003902"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR0871556.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUPM_003903"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUPM_003903"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR0871557.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="ACHUPM_003904"
     CDS             complement(2698..3390)
                     /locus_tag="ACHUPM_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFR0871558.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHUPM_003905"
     CDS             3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHUPM_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR0871559.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1366 a         1380 c         1266 g         1329 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 t
//
LOCUS       DAZPAC010000257         5297 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZPAC010000257 DAZPAC010000000
VERSION     DAZPAC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.259"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..323
                     /gene="gatA"
                     /locus_tag="ACHUPM_003906"
     CDS             <1..323
                     /gene="gatA"
                     /locus_tag="ACHUPM_003906"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR0871560.1"
                     /translation="AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQAD
                     DDNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVL
                     DSDE"
     gene            354..638
                     /gene="gatB"
                     /locus_tag="ACHUPM_003907"
     CDS             354..638
                     /gene="gatB"
                     /locus_tag="ACHUPM_003907"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR0871561.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            642..1997
                     /locus_tag="ACHUPM_003908"
     CDS             642..1997
                     /locus_tag="ACHUPM_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR0871562.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            2045..3085
                     /gene="gatD"
                     /locus_tag="ACHUPM_003909"
     CDS             2045..3085
                     /gene="gatD"
                     /locus_tag="ACHUPM_003909"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0871563.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            3191..3964
                     /locus_tag="ACHUPM_003910"
     CDS             3191..3964
                     /locus_tag="ACHUPM_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0871564.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4046..4945)
                     /gene="yegS"
                     /locus_tag="ACHUPM_003911"
     CDS             complement(4046..4945)
                     /gene="yegS"
                     /locus_tag="ACHUPM_003911"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR0871565.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
BASE COUNT         1337 a         1162 c         1349 g         1449 t
ORIGIN      
        1 aagcagaatt ccctaccggg ataatgcttg agcagcacgc cattgcaata ccgcattgtg
       61 aggcgattca tgctaagtcg tcagccattt atctgttaag gccaacaaat aaagttcatt
      121 ttcagcaagc ggatgatgat aacgacgtgg aggtatcgtt ggttattgcg ttgattgtgg
      181 agaatccgca gcagcaattg aaacttttac gctgtttatt tggcaagtta caacagcccg
      241 atatcgtcga gacactaatc actcttcctg aaacccagtt aaaggaatac ttcacaaagt
      301 atgttttaga ttcagacgaa taaatccctc tgtaacaata aaaaggacta tttatgaaac
      361 gcaagattat tgtcgcttgc ggaggcgcgg ttgcgacctc tacgatggcg gcggaagaaa
      421 ttaaagagtt gtgtcagaat catagtattc ctgttgaatt aatccagtgt cgggttaatg
      481 aaatagaaac ctatatggat ggtgtgcatt tgatatgcac cactgccaaa gtggatcgta
      541 gttttggcga tattccgtta gttcacggca tgccttttat ttctggtgtc ggtatcgaag
      601 cattacaaaa taaaattctg actatcttac aggggtgacc tatgttttca gaagtcatgc
      661 gttatattct cgacctcggc cctacggtga tgcttccgat tgtcatcatt attttttcta
      721 aaatattagg catgaaggca ggcgattgct ttaaagcggg tctgcatatc gggattggct
      781 ttgttggcat tggccttgtg attggcttaa tgctggattc cattggtccg gcggcgaaag
      841 cgatggcgga aaatttcgac ctgaatctgc atgtggtcga tgtcggctgg ccgggctctt
      901 caccaatgac ctgggcgtcg caaattgcgc tggtggcgat tccgattgcg attctggtta
      961 acgtggcgat gttactgacc cgtatgacgc gggtggtaaa tgttgatatc tggaatatct
     1021 ggcatatgac cttcaccggc gcgttgctgc atctggcaac cggttcatgg atgataggga
     1081 tggcaggtgt ggtaattcac gcggcgtttg tttataagct cggcgactgg tttgcccgcg
     1141 atacccgaaa tttctttgag ctggaaggta ttgctattcc gcacggtacg tcggcgtata
     1201 tggggccgat tgcggtgctg gtcgatgcta tcatcgagaa aatcccaggc gttaaccgaa
     1261 ttaaatttag cgccgacgat attcagcgca aatttggtcc atttggcgag cctgtcaccg
     1321 tgggttttgt gatggggctg attatcggca tcctcgccgg ttacgatgtc aaaggtgtat
     1381 tgcagctggc ggtaaaaacg gcggcagtta tgctgctgat gccacgggtg attaaaccca
     1441 tcatggatgg tttaacgccc atcgctaagc aggcccgtag tcgtttacag gcgaagttcg
     1501 gcggtcagga gttcctgatt ggcctggatc ccgcattact gctggggcat acggcggtgg
     1561 tatcggcaag cctgattttt atcccgctca ccattttaat tgctgtttgt gttccgggta
     1621 atcaggtact gccgtttggc gatcttgcca ccatcggctt cttcgtggcg atggcggtcg
     1681 cggtgcatcg tggaaatctg ttccgcacct taatctcggg tgtcatcatt atgagcatca
     1741 ccctgtggat cgcgacgcaa actattggtt tgcacaccca actggcggct aatgctgggg
     1801 cgttaaaagc cgggggtatg gtggcttcaa tggatcaggg cggttctccc attacctggt
     1861 tactgattca ggttttctcc acgcaaaata ttcccggttt cattattatc ggcgcaattt
     1921 atcttaccgg tattttcatg acctggcgta gagcgcgtgg ttttattaaa caagagaaaa
     1981 ccgttctcgc agaataattt ttacctgagg gggaggttat cccccttaac aatcaggagt
     2041 ttttatgaaa tcaatggtga atgatactga tggtatcgtg cgcgttgcag aaagcgtcat
     2101 tcctgaaatt aaacatcagg atgaggtgcg ggtaaaaatt gccagctcgg gcttatgtgg
     2161 ttccgattta cccaggatat ttaaaaatgg tgcacattat tatccaataa cgttaggcca
     2221 tgaatttagc ggctatattg atgcggtggg atccggtgtt gatgatttac accctggcga
     2281 tgcggttgcc tgtgtgccgt tattaccctg ttttacttat ccagagtgtt tgaaagggtt
     2341 ttattcccag tgcgcaaaat atgattttat tggctcgcgg cgtgatggtg gatttgctga
     2401 atatattgtc gttaagcgaa aaaatgtctt tgctctaccc acggatatgc ctattgagga
     2461 tggggctttt attgagccga ttaccgttgg cctgcatgct tttcatttag cgcaaggttg
     2521 tgagaataaa aacgttatta ttattggtgc cggaaccatt ggcctgctgg ctattcagtg
     2581 cgctgtcgcg ctgggagcaa agagtgtgac ggcgatcgac attagctcag aaaaactggc
     2641 attggcaaaa tctttcggtg cgatgcaaac atttaacagt agcgaaatga gcgcgccgca
     2701 aatgcagagc gttttacgcg aactgcgctt taatcagctt atcctcgaga cggctggcgt
     2761 accgcaaact gtcgaactgg cggtagagat tgccggtcct catgcccaac tggcgctggt
     2821 gggcacgttg catcaggatc tgcatttaac atcggcaacg tttggcaaaa tattgcgtaa
     2881 agagctgacg gttatcggca gttggatgaa ctattccagc ccttggccgg ggcaggagtg
     2941 ggaaacggcg agccggttgc tgacagaacg taagttaagc ctggagccat taatcgctca
     3001 ccgtggaagc tttgaaagct tcacccaggc ggtgcgtgac atcgctcgta atgccatgcc
     3061 gggcaaagtg ttgctcattc catgaaaccg cgggccagcg tgatgctggc ccggtattgt
     3121 gcaaaacaga ttattcacca atggtccccc ttcgtttaca ctagccacaa ttgaatatgg
     3181 gtaaatgacg atgaattcat tcgagcgaag gaataagatc atccaattag tgaatgaaca
     3241 gggaaccgtg cttgttcagg atctggcggg agtatttgct gcctcggaag cgacaatccg
     3301 tgccgatttg cgctttctcg aacaaaaagg cgtggttacg cgctttcatg gcggtgcggc
     3361 gaaaataatg tccggtaata gtgaaaccga gacccaggaa gtcgggttta aagagcgatt
     3421 tcagctcgcc agcgcgccaa aaaacagaat agcgcaggcg gcagtcaaaa tgatccatga
     3481 agggatgacc gttattctcg acagcggaag tacgacaatg cttatcgccg aaggattaat
     3541 gaccgctaaa aatatcacgg tgattaccaa cagtctcccg gcagcgtttg ccctttccga
     3601 aaataaagac attactctgg tcgtctgtgg tggcacggtc cgccataaaa cgcgctcgat
     3661 gcatggttcc attgccgagc gttcattgca ggatattaat gccgatttaa tgtttgtcgg
     3721 tgctgatggt attgatgcgg ttaatggtat tacgaccttt aatgaaggtt attcgattag
     3781 cggtgcgatg gtcaccgccg ctaataaagt gattgccgtt ctcgattcat cgaaatttaa
     3841 ccgtcgcggt tttaatcagg tactgccaat cgaaaaaatt gacattatta ttaccgatga
     3901 tgctgtatca gaggtggata aactggcatt gcagaagaca cgggtaaaat taattacggt
     3961 gtagtattgt tctgtatgcc ggataaggta tttacaccgc atccggcatt tcggattctc
     4021 cctggttcaa gagagaatag tttgattaac gcaataatag acaatctggt ggtaatcgac
     4081 aacgcaacgc cgccggaagt atttcaatat gaaagttttg tccgctcaac ggttcgccat
     4141 caagattaaa agtgatttcg tgtggggctt gtatatcaaa ccacgacgaa gcgccttcga
     4201 taatattcgg gttatcttcg tcagatttta aggttgatac gagagccgga agaatttcat
     4261 cgccggtaaa aatgcgcagt tgcagcaagc catcgttaat taacgcgttc ggacacaatt
     4321 gctgaccgcc accggcctga cgcccgttac caataccaat gaccagggcg tcaccttgcc
     4381 agtgaaagtt ttcaccgcgg atttcacaac ggtccggttg cagagtatcc atgcgcatta
     4441 agccatgaat gatgtaagag acgccaccca gcgcggcttt taatttttcc ggcgtttctg
     4501 tggtaatacg cgtcccaaat ccgcctgtcg ccatattaat aaaacaggtt tgtttgttga
     4561 cctgcgccat atctatcgca atggcgttac cggcaattgc cagtttcagc gccttatcca
     4621 gtgcctcagg aatccctaca ctggtggcaa aatcattggc ggttcctaat ggcaaaattc
     4681 ccagcgcggg tatgccatcc ccctcacact gaatcaacgc cgtagaaact tcattaatgg
     4741 tgccatcgcc accaccggca atcaccgttg cgacgcccaa cttccgggcc tcctctacat
     4801 atcgtgcggc atcgcctttc tcccaggtga cccgcacatg gatcgtcatt ccttcctcac
     4861 gcaacagcat aattgcttcg cgcaagggta gattgtcagt acttttgcca ttaagaatca
     4921 gtaagctggc gggaaattct gccatgatcg tatgtgcctt tatgattggt ctgtagatag
     4981 tgtagagcag aaaacaaaag gtggagtcag aacaggatga aagtcggagg ataaaaagtc
     5041 agttcaagta tggaggattc agtggttacc accaggagct tgcaatgggg acagcgaata
     5101 ctaacggctg ccaccgataa atttcaaaaa agagcatata cctaatattc aactaaacag
     5161 tggcatcttc aatataatat attaaagccc ccatggagtt accctgaagg gcctcaatgt
     5221 ccgtaattcc tacttatgta ggaaatgttg tacagaacat ttattataat cctattcaat
     5281 tataataatc atgccat
//
LOCUS       DAZPAC010000258         5296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZPAC010000258 DAZPAC010000000
VERSION     DAZPAC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.260"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..130)
                     /locus_tag="ACHUPM_003912"
     CDS             complement(<1..130)
                     /locus_tag="ACHUPM_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0871566.1"
                     /translation="MDTRVAFMTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPS"
     gene            356..981
                     /gene="gstB"
                     /locus_tag="ACHUPM_003913"
                     /pseudo
     CDS             356..981
                     /gene="gstB"
                     /locus_tag="ACHUPM_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(978..2093)
                     /gene="yliI"
                     /locus_tag="ACHUPM_003914"
     CDS             complement(978..2093)
                     /gene="yliI"
                     /locus_tag="ACHUPM_003914"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFR0871567.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="ACHUPM_003915"
     CDS             complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="ACHUPM_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR0871568.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2800..4125
                     /gene="rimO"
                     /locus_tag="ACHUPM_003916"
     CDS             2800..4125
                     /gene="rimO"
                     /locus_tag="ACHUPM_003916"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR0871569.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4172..5296)
                     /locus_tag="ACHUPM_003917"
                     /pseudo
     CDS             complement(4172..5296)
                     /locus_tag="ACHUPM_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         1384 a         1331 c         1261 g         1320 t
ORIGIN      
        1 cgctcggcgc ttcaacggtt tgttccgccg cgaatgccgt tggggcaaaa aggaataaaa
       61 atgcagaacc cgctgcaaga ccacgaagga gagaggagta ttgcgtcata aatgccaccc
      121 gagtatccat tcaaaaaaaa tacgttatat cgccgtatga taagttcggt gattaataac
      181 gcacacacaa acttaaagaa accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc
      241 tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa gttaacttta
      301 cttcaccatc ggagtcctgt tcagcctgtg gctgcctgtc ggaaaagggg tgagtatgat
      361 tacgctgtgg ggtcggaata attcaactaa cgtaaaaaag tattgctgac gctcgaagaa
      421 ctggaactac cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct
      481 gattttctgg cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat
      541 ctcattcttt gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa
      601 cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca
      661 aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg
      721 gaagagcgcg atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc
      781 ctgctcgatg cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc
      841 gatatcgcta tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg
      901 cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg
      961 gtgatgattc ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg
     1021 tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct
     1081 ctgtccgtta aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg
     1141 acatctttat ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg
     1201 ctgttataga aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc
     1261 tcggtcccgg cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt
     1321 ccccaggttg ccagcggcca gccgtagtgt ttgccttttt gcgggatatt aatttcatca
     1381 ccaccgcgcg ggccatgttc attcagccac agtgcattac tccacggatt catcgccatt
     1441 ccttgcggat tacgaatgcc ataagaccag atctcggcgc gcgcaccgga ttcctttata
     1501 aaaggattat catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat
     1561 ttatccagat cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa
     1621 cctttaccgt cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc
     1681 tggcggaaga cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa
     1741 cccacagcag ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg
     1801 cgagactgag caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc
     1861 caaacgtccg gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc
     1921 aactcgccgc cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc
     1981 cagggatggt cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag
     2041 agagcggaag aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct
     2101 ccggattgac tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa
     2161 aaacttaaac atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca
     2221 tggcgtgctt ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct
     2281 tatcggcgtt gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg
     2341 cggctgtcgc taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat
     2401 gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc
     2461 gtagatgatt gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg
     2521 cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa
     2581 cgaacatact tcgatcctcc tctttccagc cttgttgcga ttaaatagta tggctatttt
     2641 ttgagcaaaa tgctgtccag gatcaattct tcacgccaaa ttaacaaaat ttacatttcc
     2701 ttcgcctccg ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc
     2761 ctgccctgac ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag
     2821 ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt
     2881 ctcaccgaac tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg
     2941 gtgatcgtca acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt
     3001 ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa
     3061 gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag
     3121 caggttctgg agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc
     3181 ctggtgccag aacaaggtgt gaaactgaca ccgcgtcatt atgcctatct gaaaatttcg
     3241 gaaggctgta atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg
     3301 agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa
     3361 gagattctgg tgatctcgca ggatacttcc gcctatggcg ttgatgttaa acatcgtact
     3421 ggcttccaca acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg
     3481 aaactgggga tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc
     3541 atcccactga tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc
     3601 agcccgcgca ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc
     3661 atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc
     3721 ttccctagcg agacggaaga agatttccag atgctactcg acttcctgaa agaagcgcgt
     3781 ctggatcgcg ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg
     3841 cctgaccagg ttccggaaga agtgaaagaa gaacgctgga accgtttcat gcagctgcag
     3901 cagcagattt ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc
     3961 gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac
     4021 ggcgcggttt atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa
     4081 gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg
     4141 caaaatcatt tgtccgccct gcgaataatg attactgcca gctaatctga gccctgccgt
     4201 ttttcttact cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac
     4261 attcatttga atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat
     4321 gaacgatttc ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg
     4381 caaattcgtc accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa
     4441 ggattttcac cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa
     4501 tgtttttgaa tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt
     4561 ttaaatcttc gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg
     4621 agttatacat actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca
     4681 gtacgcgttc aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca
     4741 ctctgcgtac cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga
     4801 aaataatcaa tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc
     4861 cggtcggcaa ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag
     4921 cattatattc atcaccggtt taccgttctt aaatagagag aacatcaggt tctcgctgtc
     4981 catcgccagc gattttgaca gtaaagaaaa acgctccaga ccaagcaaaa atatcagact
     5041 acgattgtga gttaccggat agaggtaata gacgaaggtt ttaccgtcaa aagagataaa
     5101 ccctgtattt ttgacatgtc gatacttcac aacctgcata atatctgcac aataattttt
     5161 ctcgaactcc tttacctgaa tacattcgtc actggaaatc agataaggac cgacatcgta
     5221 caaataagag ggggaaaccg agtcggcgta agctgtgcgc aaaccaacca acagctgttc
     5281 ctccgtggga tttagc
//
LOCUS       DAZPAC010000259         5281 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZPAC010000259 DAZPAC010000000
VERSION     DAZPAC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.261"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..471)
                     /locus_tag="ACHUPM_003918"
     CDS             complement(<1..471)
                     /locus_tag="ACHUPM_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0871570.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
     gene            complement(471..1391)
                     /locus_tag="ACHUPM_003919"
     CDS             complement(471..1391)
                     /locus_tag="ACHUPM_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0871571.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1529..2428
                     /gene="pgrR"
                     /locus_tag="ACHUPM_003920"
     CDS             1529..2428
                     /gene="pgrR"
                     /locus_tag="ACHUPM_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR0871572.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2765..4378
                     /gene="mppA"
                     /locus_tag="ACHUPM_003921"
     CDS             2765..4378
                     /gene="mppA"
                     /locus_tag="ACHUPM_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR0871573.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTARFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4429..>5281)
                     /gene="ynaI"
                     /locus_tag="ACHUPM_003922"
     CDS             complement(4429..>5281)
                     /gene="ynaI"
                     /locus_tag="ACHUPM_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR0871574.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1430 a         1203 c         1225 g         1423 t
ORIGIN      
        1 gccaatcgga tcgtaattgc tgaaaccttc ccgaaaaaga cgcccgatat taacacccgt
       61 aatactcact actgctttaa ttcgtttttc tgtcagcgcg gcattaatgg catagccacc
      121 acccccacac actcccagca cgccaatacg ttcagcatta acatatggca gagttactgc
      181 ataatcgata acacggctaa tatcctctac gcgctgtgtc ggatcttcta tccagcgagg
      241 ctccccacca gattctccct ggaaactggc gtcataagcg agaacgagat agcctttttc
      301 agccaatgcc ttgccataga tattgccaga cgtctgctcc ttacaactgc caaaaggatg
      361 gacgctgata atcgccggat actgttttga ctcatcaaaa tttggtggaa actgaatatt
      421 ggcagcaata tgccaggcca tatcactatt gcggatttga atatgtttca ttacagagtt
      481 tcctcaaaga acggagctaa tactgaaatt gcttcagcca cataggcttt gccgtcataa
      541 agatccatat ggtttgcgcc ttcaacaatg tgatagcgtt tatcttggct tgatgctcga
      601 tcgtacaggt cgtcactcat ccatttgctc cctgcctggc tgcccgccac aatctgcatc
      661 ggctgagtca ggtacacttc cgccatatgg taagcatcaa aggtaataat ctggttaagg
      721 ctgcgcaaag tagcgtaacc cggtgctgtt ggatactgcg cgcgaggggt gtggtaatac
      781 tcccaggcct gacgcagttc ttcgttcggc gcatcggact ccttcattgg tgccagtggc
      841 ataacggcat attctccgct gctaatatcg ctggttcggg cgtttgaacc cgcttcaacg
      901 tatggcaggg catcaataga tttcacattg ttttcccaac cattacggaa catcgaacca
      961 atattgaccg cactaacggt accgatggcc ttgatgcggc gatcctgaat tgcagcattg
     1021 gctgtatatc ctgcaccggc acaaattccc atcgcaccaa ttcgggtatt gtcgacataa
     1081 gaaagcgttg tcaggtaatc aatcacggca ctgatgtctt cagtacgaat gtatgggttt
     1141 tctaactgac gcggctcgcc gccactttca ccttgataag atgcgtcata agcaatagtg
     1201 acaaatccct tttccgccag ttttttggca taggttccgg ccgtttgttc tttaacgccc
     1261 ccacctgggt gagataccac aattgcctga tactgacggg tttcatcaaa ttttggaggg
     1321 aaatagatca ctgcagacaa agagatggtt ggattattgc tgttagtgaa gctgacttta
     1381 ttattcatca ttccgttcct catggagttg tcggttcgtt ttaacggttg gtgatatcac
     1441 tatagatatt gatcattaag ttgattagac ccaaaattat gattagacct atcatttaaa
     1501 tgattaatag attgcatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc
     1561 gtttgtcgtc gttgcagagg agcgtagctt cactcgtgca gcagcccgcc tgagcatggc
     1621 gcagtcagct ttaagccaga tagtgcgtcg tatagaagaa cgattgggat tgcggcttct
     1681 gacgcgaacc acgcgcagcg ttgttccaac tgaagcgggc gagcatcttt tgtctgttct
     1741 tggccctatg ttgcatgaca tagattcagc cctggcatcc ctgagcgagc tgcagaaccg
     1801 cccatccggg acaatacgta ttactactgt agaacatgca gccaaaacga tattgttacc
     1861 agcaatgcgc acattcctga aatcgcatcc tgaaattgat attcagctca ccattgatta
     1921 tggtttgacc gatgtcgttt ctgaacgttt tgatgcaggc gtccgtctgg gtggggagat
     1981 ggataaagat atgatcgcca ttcgaatcgg gccagatata ccaatggcta ttgttggctc
     2041 accggattat ttttctcgcc gaagtgttcc aacgtcagtg tcacaattaa tagatcatca
     2101 ggcaattaat ttgtatcttc ccacatcggg tacagcaaat cgctggagat taatacgcgg
     2161 tggacgtgaa gttcgtgttc gcatggaagg tcagctttta ctgaatacga tagacctgat
     2221 cattgatgct gcaattgatg ggcatggatt ggcgtatcta ccttatgatc aggttgagcg
     2281 ggctattaaa gaaaaaaaac tgatacgtgt tttggataaa ttcacaccag atttacccgg
     2341 ttatcacctg tactatccac accgtcgaca tgctggctcg gcattctcat tatttataga
     2401 taggctgaag tataaaggtg ctgtttagca ctacttgctg atacattaat ttaatttttc
     2461 tcttaacgta ttctcagttc ctttcaacgt tttggttatt ttttattctt cgtacaatgg
     2521 cgacagatgc tgattatgat accgaaaact ggtttgaacg tgcgaagcct gaacgtagtg
     2581 ttcggtgttc tatgtgcttt accgcatttt gtcgtctatt atttacacta aatctgattt
     2641 aatatattga tacataaaac attttatgct ccctgtgtca cttttttgat ggaagttgtt
     2701 tgcatttctt taaggcgaaa caaataatta cgcatcaatt ttaatgtcgg ttagagggaa
     2761 acttatgaag cactctgttt cagtcacgtg ttgtgcgctg ttggtcagca gcatttctct
     2821 ttcgtatgct gcagaagttc cgagcggcac agtactggca gagaagcagg agctggtgcg
     2881 ccacattaaa gatgagcctg cgtcgctgga tcccgctaaa gccgtgggcc tgccagagat
     2941 tcaggtcatt cgcgatctgt ttgaaggact ggtgaatcag aacgagaaag gggagattgt
     3001 ccccggcgtt gcgactcagt ggaaaagtaa tgacaaccgt atctggactt ttaccctgcg
     3061 cgataacgca aaatgggcgg atggcacacc ggtaacggcg caagattttg tctacagctg
     3121 gcagcgtctg gtggacccga aaacgctatc gccatttgca tggtttgccg cgctggcggg
     3181 aatcaacaac gcacaggcga ttattgatgg taaagctacg ccagaccagc ttggcgtcac
     3241 cgcagttgat gcccatactt tgaaaattca gcttgataaa ccgttgccgt ggtttgtgaa
     3301 tttaaccgct aactttgcct tcttcccggt gcaaaaagcc aacgtagaaa gcggtaaaga
     3361 gtggacgaaa cccggaaatc tgatcggcaa tggcgcttat gttcttaaag atcgcgtagt
     3421 caatgaaaaa ctggtcgtgg taccgaatac ccattattgg gataacgcca aaacggtact
     3481 gcaaaaagtg accttcctgc caattaatca ggaatccgca gccactaagc gttacctcgc
     3541 gggggatatt gatatcaccg aatccttccc gaaaaatatg tatcagaagc tgttgaagga
     3601 tattccgggg caggtttata cgccgccgca gctcgggacc tattattatg cgtttaacac
     3661 gcaaaaaggg ccgacggcag atcagcgcgt tcgtctggca ttaagtatga cgatagatcg
     3721 ccgcctgatg accgaaaaag tattagggac gggcgaaaag ccagcgtggc attttacacc
     3781 agatgttacc gccagattta cgccggaacc ttcgccgttt gaacaaatga gtcaggaaga
     3841 actgaatgcg caggcaaaaa ctttgttgag cgcagctggt tatggtccgc aaaaaccgct
     3901 gaagctgacg cttttgtata acacttcaga aaaccatcaa aaaattgcga ttgctgtagc
     3961 atcgatgtgg aaaaagaacc ttggcgtaga tgttaaattg caaaatcagg aatggaaaac
     4021 ctatatcgat agccgtaaca ccggcaattt tgatgttatc cgcgcctcat gggtggggga
     4081 ttataatgaa ccctccactt tcctgacatt attaacgtca acgcattcag gaaatatttc
     4141 acgctttaac aatccggcat acgacaaagt tctggcccag gcatcgacgg aaaataccgt
     4201 taaagcgcgt aatgccgatt acaacgcggc agaaaaaatc ctcatggagc aagcaccgat
     4261 tgcaccaatt tatcaatata ccaatggacg attaatcaag ccgtggctga aaggttatcc
     4321 cattaataac ccggaagatg tggcgtacag ccggactatg tatattgtga agcattgatg
     4381 tgatgggaac tggcgttacc cttgtggata acgccagtga tgtctgattt agcgcccctg
     4441 ttccggcggt gtaatgttat ccatatacag cgtctggctg gggaaggcaa agtccgcgcc
     4501 gtgtgactgc acaatatcga taatcttcaa ataaacgtct tgctgtgcag caagccattc
     4561 agcccatacc gtggttttgg taaagcaata aaccataata ttcaatgacg agtcggcaaa
     4621 ctggttgaaa taaaccagta aggtttgtcg ctggtcgatg gccgggtgat ttttcagcat
     4681 ctcacgtacg gcttcgacaa taacgcccac ttttgccgca tcctcataac gtaaaccaat
     4741 ggtcgtggta atgcggcggt tggtcattcg tcctgggttt tctacgctga tcgacgaaaa
     4801 cagcgagttc ggtacgtaca atggacgatt atcaaaggtc gtaattttgg taattcgcca
     4861 gccaatttcc gctactgtac cttcgatatt tctgtccggt gaacggatcc agtcgccaat
     4921 actgaaagga cggtcgaaat agagcatgat cccggaaaag aaattactca gaatatcttt
     4981 accggccata ccgacagcca gaccaccaat accaccaaag gtcagcaagc cagaaaggct
     5041 catgccgaaa tgttcgccat aaagcagaac aagcaccaca ataatggtga ttttgataat
     5101 gcgcgacata atccgcgcac tggtgatatc gcgacctttt ttaatctgct gtttttcaaa
     5161 ctgattaatc agcaaaaata gcttaatcgt cagaataacc gcaatcaggg acgtacagat
     5221 aaaatcgata acgtctgggg tgataaattt gagtttatag ttttctataa cataattaat
     5281 a
//
LOCUS       DAZPAC010000260         5261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZPAC010000260 DAZPAC010000000
VERSION     DAZPAC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.262"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1278
                     /gene="ipaB"
                     /locus_tag="ACHUPM_003923"
     CDS             <1..1278
                     /gene="ipaB"
                     /locus_tag="ACHUPM_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFR0871575.1"
                     /translation="NKINQIQTRLSELDPDSPEKKKLSREEIQLTIKKDAAVKDRTLI
                     EQKTLSIHSKLTDKSMQLEKEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFI
                     QLVGKNNEESLKNDLALFQSLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILG
                     ALLTIVSVVAAAFSGGASLALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEP
                     LIKLLSDAFTKMLEGLGVDSKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLA
                     ENIGKIIGKTLTDLIPKFLKNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHL
                     NQAVLLGESVNSATQAGGSVASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFG
                     QLQEVIADLLASMSNSQANRTDVAKAILQQTTA"
     gene            1241..2389
                     /gene="ipaC"
                     /locus_tag="ACHUPM_003924"
     CDS             1241..2389
                     /gene="ipaC"
                     /locus_tag="ACHUPM_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFR0871576.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKSTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMDTSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSITQSRNSTASQIAGNIRA"
     gene            2440..3438
                     /gene="ipaD"
                     /locus_tag="ACHUPM_003925"
     CDS             2440..3438
                     /gene="ipaD"
                     /locus_tag="ACHUPM_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFR0871577.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKKKYEDKPLYPATNTV
                     SQKEADKWLTELGGTIGKVSKKNGGYVVNINMTPIDNMLKSLNNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            3447..>5261
                     /gene="ipaA"
                     /locus_tag="ACHUPM_003926"
     CDS             3447..>5261
                     /gene="ipaA"
                     /locus_tag="ACHUPM_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFR0871578.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKESFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFFSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDI"
BASE COUNT         1940 a         1070 c          894 g         1357 t
ORIGIN      
        1 aataaaatta accaaattca aacaagatta tccgaactcg acccagattc accagaaaag
       61 aaaaaattaa gccgggaaga aatacaactc actatcaaaa aagacgcagc agttaaagac
      121 aggacattga ttgagcagaa aaccctgtca attcatagca aacttacaga taaatcaatg
      181 caactcgaaa aagaaataga ctctttttct gcattttcaa acacagcatc tgctgaacag
      241 ctatcaaccc agcagaaatc attaaccgga cttgccagtg ttactcaatt gatggcaacc
      301 tttattcaac tagttggaaa aaataatgaa gaatctttaa aaaatgatct ggctctattc
      361 cagtctctcc aagaatcaag aaaaactgaa atggagagaa aatctgatga gtatgctgct
      421 gaagtacgta aagcagaaga actcaacaga gtaatgggtt gtgttgggaa aatacttggg
      481 gcacttttaa ctatcgttag tgttgttgca gcagcttttt ctggaggagc ctctctagca
      541 ctggcagctg ttggtttagc tcttatggtt acggatgcta tagtacaagc agcgaccggc
      601 aattccttca tggaacaagc cctgaatccg atcatgaaag cagtcattga acccttaatc
      661 aaactccttt cagatgcatt tacaaaaatg ctcgaaggct tgggcgtcga ctcgaaaaaa
      721 gccaaaatga ttggctctat tctgggggca atcgcaggcg ctcttgtcct ggttgcagca
      781 gtagttctcg tagccactgt tggtaaacag gcagcagcaa aacttgcaga gaatattggc
      841 aaaataatag gtaaaaccct cacagacctt ataccaaagt ttctcaagaa tttttcttct
      901 caactggacg atttaatcac taatgctgtt gccagattaa ataaatttct tggtgcagcg
      961 ggtgatgaag taatatccaa acaaattatt tccacccatt taaaccaagc agttttatta
     1021 ggagaaagtg ttaactctgc cacacaagcg ggaggaagtg tcgcttctgc tgttttccag
     1081 aacagcgcgt ctacaaatct agcagacctg acattatcga aatatcaagt tgaacaactg
     1141 tcaaaatata tcagtgaagc aatagaaaaa ttcggccaat tgcaggaagt aattgcagat
     1201 ctattagcct caatgtccaa ctctcaggct aatagaactg atgttgcaaa agcaattttg
     1261 caacaaacta ctgcttgata caaataagga gaatgttatg gaaattcaaa acacaaaatc
     1321 aacccagatt ttatatacag atatatccac aaaacaaact caaagttctt ccgaaacaca
     1381 aaaatcacaa aattatcagc agattgcagc gcatattcca cttaatgtcg gtaaaaatcc
     1441 cgtattaaca accacattaa atgatgatca acttttaaag ttatcagagc aggttcagca
     1501 tgattcagaa atcattgctc gccttactga caaaaagatg aaagatcttt cagagatgag
     1561 tcacaccctt actccagaga acactctgga tatttccagt ctttcttcta atgctgtttc
     1621 tttaattatt agtgtagccg ttctactttc tgctctccgc actgcagaaa ctaaattggg
     1681 ctctcaattg tcattgattg cgttcgatgc tacaaaatca gctgcagaga acattgttcg
     1741 gcaaggcctg gcagccctat catcaagcat tactggagca gtcacacaag taggtataac
     1801 gggtatcggt gccaaaaaaa cgcattcagg gattagcgac caaaaaggag ccttaagaaa
     1861 gaaccttgcc actgctcaat ctcttgaaaa agagcttgca ggttctaaat tagggttaaa
     1921 taaacaaata gatacaaata tcacctcacc acaaactaac tctagcacaa aatttttagg
     1981 taaaaataaa ctggcgccag ataatatatc cctgtcaact gaacataaaa cttctcttag
     2041 ttctcccgat atttctttgc aggataaaat tgacacccag agaagaactt acgagctcaa
     2101 taccctttct gcgcagcaaa aacaaaatat tggccgtgca acaatggaca catcagccgt
     2161 tgctggtaat atatccacat caggagggcg ttatgcatct gctcttgaag aagaagaaca
     2221 actaatcagt caggccagca gtaaacaagc agaggaagca tcccaagtat ctaaagaagc
     2281 atcccaagcg acaaatcaat taatacaaaa attattgaat ataattgaca gcatcaccca
     2341 atcaaggaat tcgacagcca gtcagattgc tggtaacatt cgagcttaat atatccaaga
     2401 gccataataa tatatggctc ttcctgtaag gaaataacca tgaatataac aactctgact
     2461 aatagtattt ccacctcatc attcagtcca aacaatacca acggttcatc aaccgaaaca
     2521 gttaattctg atataaaaac aacgaccagt tctcatcctg taagttccct tactatgctc
     2581 aacgacaccc ttcataatat cagaacaaca aatcaggcat taaagaaaga gctttcacaa
     2641 aaaacgttga ctaaaacatc gctagaagaa atagcattac attcatctca gattagcatg
     2701 gatgtaaata aatccgctca actattggat attctttcca ggaacgaata tccaattaat
     2761 aaagacgcaa gagaattatt acattcagcc ccgaaagaag ccgagcttga tggagatcaa
     2821 atgatatctc atagagaact gtgggctaaa attgcaaact ccatcaatga tattaatgaa
     2881 cagtatctga aagtatatga acatgccgtt agttcatata ctcaaatgta tcaagatttt
     2941 agcgctgttc tttccagtct tgccggctgg atctctcccg gaggtaacga cggaaactcc
     3001 gtgaaattac aagtcaactc gcttaaaaaa gcattggaag aactcaagaa aaaatatgaa
     3061 gataaaccgc tatatccagc aactaatact gttagtcaga aagaagcaga taaatggctt
     3121 acagaattag gtggaacaat cggcaaggta tctaaaaaaa acgggggata tgttgtcaat
     3181 ataaacatga ccccaataga caatatgtta aaaagcttaa ataatctagg tggaaatggc
     3241 gaggttgtgc tagataatgc aaaatatcag gcatggaatg ccggattctc tgccgaagat
     3301 gaaacaatga aaaataatct tcaaacttta gttcaaaaat acagtaatgc caatagtatt
     3361 tttgataatt tagtaaaggt tttgagtagt acaataagct catgtacaga tacagataaa
     3421 ctttttctcc atttctgagg tgcgccatgc ataatgtaaa taatactcaa gcgccaacat
     3481 tcttatataa ggcaacttca ccatcatcaa cagaatacag cgagttaaaa agcaaaatat
     3541 ccgatatcca tagttcgcaa acttctctaa aaacaccagc atcagtgtct gaaaaagaaa
     3601 gctttgcaac gtcttttaat cagaaatgtc ttgatttttt attttcttcc tcagggaaag
     3661 aagatgtgtt aagaagcatt tattccaact caatgaatgc gtatgccaaa agcgagattc
     3721 tcgaattttc aaatgttttg tactccttag tacatcaaaa tggtcttaat tttgaaaacg
     3781 aaaagggact tcaaaaaatt gtcgcacagt attcggaact aattataaaa gataaattat
     3841 cccaagattc tgcctttgga ccatggtcgg caaagaataa gaaactccat caattacgac
     3901 aaaacattga gcacagactt gcactattag cacaacaaca cacatctggt gaagctttat
     3961 cattgggaca aaaactcctc aatactgaag tatcatcatt tatcaagaat aatattcttg
     4021 ctgaattaaa gttaagtaat gaaactgttt catctctcaa actagatgat ttagttgacg
     4081 cacaggcaaa acttgccttt gatagtttgc gcaatcaacg taaaaatact attgatagta
     4141 aaggatttgg tataggtaaa ctgtcaagag acttaaatac agtagccgtg tttcctgagc
     4201 tgttgagaaa agtccttaat gatattttag aagatataaa agattcgcat cctatccaag
     4261 atggcctccc tacacctccc gaagatatgc cagatggcgg accaaccccc ggagccaatg
     4321 agaaaacatc ccaacctgta attcactatc atataaataa tgataataga acttacgata
     4381 atagagtttt tgacaacaga gtatatgaca atagctatca cgagaaccca gaaaatgatg
     4441 cacagtctcc tacttctcag acaaacgatc tattatcccg taacggaaac tcattactaa
     4501 atccacaaag agcactagtt caaaaagtaa cttccgttct accacactct atatcagata
     4561 ctgtccagac atttgcaaat aattcagctt tagaaaaggt tttcaaccat actccagata
     4621 attcggatgg aataggttca gacctgttaa ctacgagtag tcaagaaaga tctgcaaata
     4681 actctctttc tcggggacac aggcctctga acatacagaa ctcttcaacc accccccctc
     4741 tccacccgga aggagtgaca agcagtaatg ataactcatc agatacaact aaaagtagcg
     4801 cttctctttc tcatagagta gcttcgcaaa tcaataaatt caactcaaac actgattcaa
     4861 aagtacttca gactgatttt ttctcaagaa atggagacac atatttaaca cgggaaacga
     4921 tatttgaagc gtcaaaaaaa gtaacaaact ccctaagtaa tcttatatct ctcattggaa
     4981 ctaaatcagg aacacaagaa cgagagttac aggaaaaatc aaaggacatt acaaaatcca
     5041 caacagaaca cagaataaac aacaaattaa aagttacaga tgcaaataca ataaactacg
     5101 taacagaaac caacgcagat acaattgata aaaatcatgc gatctatgaa aaggcaaaag
     5161 aagtatctag cgccctcagc aaggtattgt caaaaattga cgatacctct gcagaattac
     5221 ttacagatga tatatctgat ttaaaaaata acaatgatat t
//
LOCUS       DAZPAC010000261         5261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZPAC010000261 DAZPAC010000000
VERSION     DAZPAC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.263"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..69
                     /locus_tag="ACHUPM_003927"
     CDS             <1..69
                     /locus_tag="ACHUPM_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871579.1"
                     /translation="VYWVIAVISVVALFLTAKVKDV"
     gene            complement(118..723)
                     /gene="leuD"
                     /locus_tag="ACHUPM_003928"
     CDS             complement(118..723)
                     /gene="leuD"
                     /locus_tag="ACHUPM_003928"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR0871580.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(734..2134)
                     /gene="leuC"
                     /locus_tag="ACHUPM_003929"
     CDS             complement(734..2134)
                     /gene="leuC"
                     /locus_tag="ACHUPM_003929"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR0871581.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(2137..3228)
                     /gene="leuB"
                     /locus_tag="ACHUPM_003930"
     CDS             complement(2137..3228)
                     /gene="leuB"
                     /locus_tag="ACHUPM_003930"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR0871582.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(3228..4799)
                     /gene="leuA"
                     /locus_tag="ACHUPM_003931"
     CDS             complement(3228..4799)
                     /gene="leuA"
                     /locus_tag="ACHUPM_003931"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR0871583.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDITTAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(4892..4978)
                     /gene="leuL"
                     /locus_tag="ACHUPM_003932"
     CDS             complement(4892..4978)
                     /gene="leuL"
                     /locus_tag="ACHUPM_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR0871584.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         1139 a         1393 c         1396 g         1333 t
ORIGIN      
        1 gtctactggg taattgcggt tatttctgtt gtcgcattat ttttaaccgc aaaggttaaa
       61 gacgtttgat gacgtggacg atagcggaaa gcccggtcat ttgaccgggc aaggggatta
      121 attcataaac gcaggttgtt ttgcttcata agcggcaatg gcgtcgtcgt gctgcaaggt
      181 aagcccaata ctgtccagac cgttcatcat gcagtggcgg cggaaggcat cgatggtaaa
      241 gcgataggtt ttctctcccg ctttcacctc ttgcgcttcc agatccacgt cgaaatggat
      301 ccccggattg gctttcacca gcgcaaacag ttcgtccact tctgcatcgc ttaatttcac
      361 cggcagcagc tggttgttaa agctattgcc gtagaagatg tcagcaaaac tcggcgcaat
      421 caccacttta aagccgtagt cggtcaatgc ccagggcgcg tgctcacgcg aggagccaca
      481 gccgaagttt tctcgtgcca gcaaaatgga agcgccctga tactgcggga agttcagcac
      541 gaagtccggg tttggctgtt ggcctttttc atcgagaaaa cgccagtcgt taaacagatg
      601 cgcgccaaaa cccgtacggg tcactttctg caaaaactgt ttcgggatga ttgcatcggt
      661 atcgacattg gcggcatcca gcggaaccac caggcctgtg tgtttgataa atttctctgc
      721 catggtgtgc tccttattta atgttgcgaa tgtcggcgaa atgtccggtc acagcagcag
      781 cggcagccat tgccgggctg accagatgcg tgcgcccgcc gcgcccctgg cggccttcaa
      841 agttacggtt gctggtggat gcacaacgtt cgcccggatt cagacggtcg ttgttcatcg
      901 ccagacacat tgagcagcca ggcaagcgcc attcaaaacc ggcttcaata aagattttat
      961 ccagaccttc cgcttccgcc tgggctttta ccgggccaga gccgggaacc accagtgcct
     1021 gcacgcctgg cgcgactttt cgccctttgg cgatctccgc tgccgcgcgt aaatcttcaa
     1081 tgcgcgagtt ggtacaggaa ccgataaaca ctttgtcgat agccacttcg gtcagcggaa
     1141 tgcccggttt cagccccata taggccagcg ctttttctgc cgacgcgcgt ttaaccggat
     1201 cggcaaacga agccggatcg ggaatattgt cgttcacgga aatcacctgg cctggattgg
     1261 tgccccaggt gacctgcggt gaaatttctt ctgcttgcag agtgacaacg gtatcgaaag
     1321 ttgcgccttc gtcggtttgc agggttttcc agtaggcaac ggcgtcgtcg aaatctttgc
     1381 ctttcggcgc atgcaaacgg cctttgacat agttaaaggt ggtttcgtcc ggtgcaacca
     1441 gaccggtttt tgcgcccatt tcgattgcca tattgcacag ggtcatacga ccttccatgc
     1501 ttaaatcacg gattgcttcg ccgcaaaact ccaccacatg cccggtgccg cctgcgctac
     1561 cggttttacc gataattgcc agcacgatat cttttgcggt aatgcccggc gcggctttgc
     1621 cctggacttc aattttcatg gtttttgcgc ggccctgttt cagggtttgc gttgccagta
     1681 cgtgttcaac ttcggaagtg ccgataccaa aggccagtgc gccaaacgcg ccgtgggtgg
     1741 cggtatgcga gtcgccgcag acaatggtca tccccggcaa ggtgacgccc tgttccggcc
     1801 ccattacgtg gacgatcccc tgatacgggt gattcaggtc atacagttcg acgccaaatt
     1861 ctttgcagtt tttaatcagc tcctgcatct ggatgcgcgc catttcacca caggcattaa
     1921 tgtctttcgt ctgggtagag acgttgtgat ccatagtggc gaaggttttg cccggctgac
     1981 gtaccgggcg accgtgggcg cgcagaccat tgaacgcctg cggtgaggtc acttcatgca
     2041 ccaggtggcg gtcgatatat aacagcgggg tttcgttttc ggcttcgtac actacgtgag
     2101 cgtcgaataa tttttcgtat aacgttttag ccatgattac accccttctg ccacatagcg
     2161 ggcaatgata tcgcccattt catcggtact aacggcggca gcgccacggg ctaaatctcc
     2221 ggtgcgaatg ccttcttcta atgcgcggtt aatggcgcgt tcaatggcgc aagccgcatc
     2281 atcggcatcc aggctgtagc gcagcagcag tgccagcgac agaatttgcg caatcgggtt
     2341 ggcgatgttt ttgcctgcga tatctggtgc tgagccgcct gccggttcat acagaccaaa
     2401 accttgctcg ttcaggctgg cggaaggcaa catccccatc gagccagtga tcattgcgca
     2461 ctcgtcagac agaatgtcgc caaacaggtt ggagcacagc agaacgtcaa actgtgatgg
     2521 atctttaatc agctgcatgg tggcgttgtc gatgtacata tgcgccagtt cgacatccgg
     2581 gtattccgtg gcgatctcgt tgacgatttc acgccataaa atagaggatt gcagcacgtt
     2641 agctttgtcg attgaggtga ctttgtggcg acgtttacga gcagattcaa acgcgatgcg
     2701 ggcaatacgc tcgatctcaa aacggtgata cacctcggta tcaaacgctt tttcatattg
     2761 tccgctacct tcgcggcctt ttggctgacc gaaatagatg ccgccggtca gttcgcgcac
     2821 gcacaggatg tcgaagccgt tagcggcaat gtctgcacgc agcggacaga atgcttccag
     2881 cccctgatac agttttgccg ggcgcaggtt gctgaataat ttgaagcgct tacgcagagg
     2941 cagcagcgcg ccgcgttctg gttgctggtc tggtggtaaa tgttcccact tcgggccgcc
     3001 taccgagcca aacagcacgg catcggcttg ctcacaacct tcaaccgtcg caggcggcag
     3061 tggttgcccg tggttatcaa tggctgcgcc gcctacatcg taatggctgg tggtgatgcg
     3121 catcgcaaag cggttgcgca cggcattcag cactttcagc gcctgggtca tcacttccgg
     3181 accaataccg tcccccggca atacggcaat atggtaattc ttcgacatca cacggtttcc
     3241 ttgttgtttt cgttgtgttg agctttgcgt tgcaactctt tttcgacttc tgcggcacgc
     3301 cagatattgt tcagtacgtg caccatggct ttggcggagg actcgacaat atcggtggcc
     3361 aggccgacgc cgtggaagcg gcgaccgttg tagttggcga caatatccac ctgacccagc
     3421 gcatctttac cgtgaccttt ggcggtcagg ctgtatttca ccagttcgac gttatagtca
     3481 gtgatgcggt taattgcctg atagacggca tcgaccggac cgttaccgtt ggcggcttct
     3541 gctttgactt cttcgccaca ggccagtttg acggcggcgg tggtgatatc gttagagcca
     3601 gactgcacgc tgaagtaatc cagacggaaa tgctccggct cttcttgctg cttaccgatg
     3661 aaggccagcg cctccagatc gtaatcaaac acctgacctt ttttgtccgc cagcttcagg
     3721 aaagcatcgt acaaattgtc taaattatat tcactttctt tataccccat ctcatccatg
     3781 cgatgtttca ccgccgcacg ccccgaacga gaggtcagat tcagctggat ttggttcaga
     3841 ccaatagatt ctggtgtcat gatttcgtag ttttcgcggt ttttcagcac gccatcctgg
     3901 tggataccgg aggagtgagc gaatgcgctg ctgccaacaa tggctttgtt tgccgggatc
     3961 ggcatattac aaatctggct aactaactgg ctggtgcgcc atatctcctg gtgattaatg
     4021 gcggtgtgga cgttgagaat atccttacga actttaatcg ccatgatgac ttcttccagc
     4081 gaacagttac cggcacgctc gccgatcccg ttcattgcac cttccacctg gcgtgcaccg
     4141 gcatgtaccg ccgccagtga gttgccgacc gccaggccta aatcgtcgtg ggtatgtacg
     4201 gaaataatgg ctttgtcgat gttaggtacg cgttcataca ggccgctgat gattccggcg
     4261 aactcaaacg gcatggtgta gcccacggtg tccggaatgt tgatggtggt ggcaccggca
     4321 ttaatcgccg cttcgaccac tcgcgccaga tcggcaatgg gtgtacgccc ggcatcttcg
     4381 caagaaaatt caacatcatc ggtgtaatta cgggcgcgtt tcaccatata gatagcgcgt
     4441 tcgatcacct cgtccagggt gctgcgcagc ttggtggcga tgtgcattgg ggaggtagca
     4501 ataaaggtat gaatacggaa ggcttcggcg actttcaggg attcggccgc cacgtcgata
     4561 tctttttcca cgcagcgagc taacgcacat acgcggctgt ttttaacctg gcgggcgatg
     4621 gtttgcaccg attcaaaatc gcccggcgaa gagacgggga aaccgacttc catcacgtca
     4681 acacccatac gctcaagggc cagcgcaatt tgcagttttt ctttcacact caagcttgcc
     4741 tgtaacgcct gttcaccgtc gcgcaatgtg gtatcgaaaa taatgacttg ctggctcatg
     4801 gtttaggtcc ttgtctcttt tagagcgcct cgcttcgggc ataaaaaaac ccgcgcaatg
     4861 gcgcgggttt tttgtttgac tgcgtgctgg cttaatgctg gatgccgctc actcgtctac
     4921 cgcgcaaaga agatgcgttt agtagtagta gaccgataaa gcgaacgatg tgagtcatta
     4981 aatcagctcc agatgaatgc gatatgcttt tagagttact ggatacaaaa acggatgtca
     5041 accctgacgc aataaaaacg tcccgccagc gtgagttctg catccgtaaa attagctaat
     5101 tgtgctgcgg tggttaaagt aagcgatatt aatttctgct taactaccga cgcttttcat
     5161 cggttgacat atttcagcat aaatttttgc atctaatcaa cgaggaaaaa ggggacaaaa
     5221 tgcacgcgtt gcaaaaccta tcctgatgat ttgtattgaa t
//
LOCUS       DAZPAC010000262         5240 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZPAC010000262 DAZPAC010000000
VERSION     DAZPAC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.264"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..252
                     /locus_tag="ACHUPM_003933"
     CDS             <1..252
                     /locus_tag="ACHUPM_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR0871585.1"
                     /translation="SDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAY
                     EAWQQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(261..659)
                     /locus_tag="ACHUPM_003934"
     CDS             complement(261..659)
                     /locus_tag="ACHUPM_003934"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFR0871586.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(659..886)
                     /gene="vapB"
                     /locus_tag="ACHUPM_003935"
     CDS             complement(659..886)
                     /gene="vapB"
                     /locus_tag="ACHUPM_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFR0871587.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            968..>5240
                     /gene="traI"
                     /locus_tag="ACHUPM_003936"
     CDS             968..>5240
                     /gene="traI"
                     /locus_tag="ACHUPM_003936"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFR0871588.1"
                     /translation="MMSIAQVRSAGSADNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVTNVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKRKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     MYTDLLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLT
                     LRNAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDHFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     VGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLERVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRK"
BASE COUNT         1179 a         1356 c         1682 g         1023 t
ORIGIN      
        1 tcagattcag gtgtgaatgt tccggcaggg gggattgagc aggagctgaa aatgaaaccg
       61 gaagaagaga tggaacagca actgccaccc gggatcagtg aatccggtga agtggtggat
      121 atggccgctt atgaggcatg gcagcaggaa aatcatccgg acacctggca gcagatgcag
      181 cgtcgtgaag aggtgaacat caatgtgcac cgggaacgcg gggaggatgt tgagccggga
      241 gatgatttct gatgaacagg tcagctccag tcttcagttc tcaggccgcc cacacgttca
      301 aattcccggg tgttattagt cacaataatc agcccccgac tgcgggcatg acctgcgatc
      361 atttgatcaa atggcccgac agggcgtccc tgacgggcaa gttctgctct tatctggccg
      421 gtgtgtgtgg ctgcagcagc gtcgtaatcc agaacatcaa tgcgggaaac aaatccctcg
      481 atcacagcga gattacgttc aggcatctgg cttttttctg caccatatat cagctccatc
      541 agggtgaccg aactgataca cattctcccc tggttcaggt taaaacgctc cctgacgctg
      601 gcgggtttgt tctttatcgt aaaaatgcag atgttggtat cgagcataaa cttcagcatc
      661 agaatgactc cctttcctgc atgccgggct gttccctgtt atccataaaa tcggcgctga
      721 cgctgtgtcc gtcgaaccat tcgtcccatg tctctccggc tggcgtaatg attctggttc
      781 gtccgacagc aatcacttca acgcgtttta cgttttccgg caatgcaacc gcttttggca
      841 gtctgaccgc ctggctgcgg ttgctgagaa atacggtggt ttccattatt tgcctcctta
      901 tgtggatatg tcttgtgtat atctattgtg ggattgatgc cgggatatgt caaagggata
      961 tacgtttatg atgagtatcg cgcaggtcag atcggccgga agtgccgata attattatac
     1021 cgacaaggat aattactatg tgctgggcag catgggagaa cgctgggccg gccagggggc
     1081 tgaacaactg gggctgcagg gcagtgtcga taaggatgtc tttacccgcc ttctggaggg
     1141 caggctgccg gacggggcgg atttaagccg catgcaggat ggcagtaaca ggcatcgtcc
     1201 cggctacgac ctgaccttct ccgctcccaa aagtgtctcc atgatggcca tgctcggggg
     1261 ggacaaacgt cttattgaag cacataacca ggccgtggat tttgctgtcc gtcaggtgga
     1321 ggcgctggct tccacacggg tgatgacgga cggacagtca gaaacggtgc tgaccggtaa
     1381 tctggtgatg gcactgttta accacgacac cagtcgcgat caggaaccac agttacacac
     1441 gcatgcggtg gtgactaatg ttacgcagca taacggcgaa tggaagacac tgagcagtga
     1501 caaagtgggg aaaacggggt tcattgagaa tgtgtacgct aatcagattg cctttggcag
     1561 gctctaccgg gaaaaacgga aagagcaggt tgaggcgctg ggctatgaaa ctgaagtggt
     1621 cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg gaggcctttt ccggacgctc
     1681 acagactatc cgggaggccg tcggggaaga cgcctcgctg aaatcccggg atgtggcggc
     1741 gctggatacg cgtaaatcca aacagcacgt cgatcctgaa gtcagaatgg ccgagtggat
     1801 gcagacgctg aaggaaaccg ggttcgacat ccgggcatat cgtgacgcag cggaacaacg
     1861 ggcatatacc cgcacgcaga cacccggacc cgcttcacag gacgggccgg atgtgcagca
     1921 ggcggtgaca caggccattg ccggattaag tgaacgcaaa gtgcagttta tgtatacgga
     1981 cctgctggcc aggacggttg gcatactgcc gccggaaaat ggtgtgattg aacgggcacg
     2041 cgccggtatc gatgaggcca tcagccgtga gcagcttatc cccctcgatc gtgagaaggg
     2101 gctgttcacg tccgggattc atatgctcga tgagctgtca gtccgggcac tcagtcgtga
     2161 catcatgaaa cagaaccggg tgaccgtaca tccggagaaa agtgtccccc ggacggccgg
     2221 ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg tcgctggcca ttgtgtccgg
     2281 gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa ctggtcatga tggcccggga
     2341 gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc tcgcagatga acctgaagca
     2401 ggatgaacgg ctgtccggtg aactgataac cggacgtcgt cagttgctgg aaggcatggc
     2461 cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa aaactctccc tgaaagagac
     2521 gttaaccctg ctggatggtg ccgcacgtca taacgtacag gtcctgataa ccgacagcgg
     2581 gcagcgaacc ggtacaggca gtgcactgat ggccatgaag gatgccgggg tgaacacata
     2641 tcgctggcag gggggagaac agcgaccggc caccatcatc agtgaaccgg accgtaatgt
     2701 ccgctatgcc cggctggccg gagattttgc ggccagcgtg aaagccggag aagagagcgt
     2761 ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc gcagtgagct
     2821 gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt caccggtctg
     2881 gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg tgatggagca
     2941 gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga cggcgcagag
     3001 tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta tttcttccct
     3061 ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg gtgagcgtct
     3121 gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc tgcaggtggc
     3181 atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac cggccaccct
     3241 gcctgtgagc gattcaccgt tcacggcact gaagctggag aacggctggg tggaaacgcc
     3301 cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga tggcaatgga
     3361 caatgccacc ctgaacggtc tggcccgcag tggtcgtgat gtccggctgt attcctcact
     3421 ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg tggtttctga
     3481 gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc tgcagaaagc
     3541 cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc tggaaagtaa
     3601 aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg ctgcagaagg
     3661 aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg gtgatttact
     3721 gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg cgtcgtatga
     3781 ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca cgccgctgat
     3841 ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg ccgccacccg
     3901 tatgatactg gaaacgtccg accatttcac ggtggtgcag ggctatgccg gtgtgggtaa
     3961 gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga gtgaacgtcc
     4021 ccgtgtcgtt gggctgggtc ccacgcaccg cgcggtcggg gagatgcgca gcgccggcgt
     4081 ggatgcgcag acactggcgt cctttctgca tgacacgcag ctgcagcagc gcagcggaga
     4141 aacgccggat ttcagcaaca cgctgttcct gctcgatgag agctcaatgg tgggcaatac
     4201 cgacatggca cgggcatacg ccctgattgc ggtcggtggc ggtcgggctg tggccagcgg
     4261 tgacacggac cagctgcagg ccatcgcgcc tggtcagcct ttccgtctcc agcagacgcg
     4321 cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg aactgcggga
     4381 ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc ttgagcgtgt
     4441 gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact ccgtgacgga
     4501 gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga tgctgaaagg
     4561 cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg actataccgg
     4621 caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg aggatcggcg
     4681 cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg ggcaggtgca
     4741 ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc gtcgtctctc
     4801 cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc accggattgc
     4861 cggtatcagc aaggatgacg ggctgataac cctgcaggat gcggaaggta acacgcggct
     4921 gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg acaccatccg
     4981 ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg gttatgtggc
     5041 caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt ctgacggtca
     5101 gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg acctggccta
     5161 tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg cgcttgaagg
     5221 cacggaaggt aaccggaaac
//
LOCUS       DAZPAC010000263         5228 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZPAC010000263 DAZPAC010000000
VERSION     DAZPAC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.265"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(1..1133)
                     /locus_tag="ACHUPM_003937"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="ACHUPM_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHUPM_003938"
     CDS             complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHUPM_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFR0871589.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="ACHUPM_003939"
     CDS             complement(1327..3060)
                     /locus_tag="ACHUPM_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR0871590.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="ACHUPM_003940"
     CDS             complement(3057..3551)
                     /locus_tag="ACHUPM_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR0871591.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="ACHUPM_003941"
     CDS             complement(3673..4023)
                     /locus_tag="ACHUPM_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0871592.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="ACHUPM_003942"
     CDS             complement(4066..4530)
                     /locus_tag="ACHUPM_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0871593.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="ACHUPM_003943"
     CDS             complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="ACHUPM_003943"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFR0871594.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(5024..>5228)
                     /locus_tag="ACHUPM_003944"
     CDS             complement(5024..>5228)
                     /locus_tag="ACHUPM_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR0871595.1"
                     /translation="EKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGSLLLG
                     LLTYLTNLYFKIREDKRKAARGE"
BASE COUNT         1143 a         1529 c         1298 g         1258 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgcttaccgc cagcatatgt ccagcgacgt
     4621 agctggtcac atgcgccttt gatatcgccc tggtttattt tgcgaagaag cgtcgatgtt
     4681 ctgaaattgc cagcgcccac gttgtaaacg aacgagtaaa gagcgccgcg cgttgtttcc
     4741 ggtatatcga ctttgatgta cgggttaatt tgtctggcga cagtggcaag gtctttattc
     4801 aggagggctt tgcattctgc ttcggtatac gttttaccga gcatgatgtc ttttccggtg
     4861 tgtccgtgac atacagtcca tacaccaatg atatctttgt atggtatgta gctgacacct
     4921 tccagaccat cgttaccacc tgggccagtg attaacacag atgctatagc aacagcccca
     4981 ccaccaatag cagctgcaac agccttgcgt aatgatggcg acattattca cctctcgcag
     5041 ccttacgctt gtcttctctt attttgaaat acagatttgt cagataagtc agaagcccca
     5101 gaagcagact tcccagcaca ccaatcgcag cccactgtga tggactgacc tgatccaacc
     5161 actgcaaaaa ccagtagccg gcactgccgg cggaggtgcc gtaggcaatg cctgttgata
     5221 ttttttcc
//
LOCUS       DAZPAC010000264         5219 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZPAC010000264 DAZPAC010000000
VERSION     DAZPAC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.266"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..227
                     /locus_tag="ACHUPM_003945"
     CDS             <1..227
                     /locus_tag="ACHUPM_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871596.1"
                     /translation="KNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPA
                     IIHSALNPTHYYQPVNDETTVMEIIKAYSK"
     gene            complement(291..575)
                     /locus_tag="ACHUPM_003946"
     CDS             complement(291..575)
                     /locus_tag="ACHUPM_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Z C-terminal domain-related protein"
                     /protein_id="HFR0871597.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIA
                     FIWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
     gene            complement(593..790)
                     /locus_tag="ACHUPM_003947"
     CDS             complement(593..790)
                     /locus_tag="ACHUPM_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871598.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSV
                     SGLDVELIDVSCESEWEEHSH"
     gene            1002..1298
                     /locus_tag="ACHUPM_003948"
     CDS             1002..1298
                     /locus_tag="ACHUPM_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871599.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLT
                     QADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
     gene            complement(1843..2076)
                     /locus_tag="ACHUPM_003949"
     CDS             complement(1843..2076)
                     /locus_tag="ACHUPM_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR0871600.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(2083..2613)
                     /locus_tag="ACHUPM_003950"
     CDS             complement(2083..2613)
                     /locus_tag="ACHUPM_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR0871601.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETILNDEFRKLEKSVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            complement(2651..3349)
                     /locus_tag="ACHUPM_003951"
                     /pseudo
     CDS             complement(2651..3349)
                     /locus_tag="ACHUPM_003951"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3291..3614)
                     /locus_tag="ACHUPM_003952"
     CDS             complement(3291..3614)
                     /locus_tag="ACHUPM_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR0871602.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            3679..3870
                     /locus_tag="ACHUPM_003953"
     CDS             3679..3870
                     /locus_tag="ACHUPM_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR0871603.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            4538..4651
                     /locus_tag="ACHUPM_003954"
     CDS             4538..4651
                     /locus_tag="ACHUPM_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0871604.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
BASE COUNT         1373 a         1427 c         1116 g         1303 t
ORIGIN      
        1 aaaaaaacaa aggcgtcata atagaagtaa acacagatag tttatgtaag aaaaaactaa
       61 acgaaataac tagtaaaaat atagggaaac ccctgatttc atattttgat ggaaaagtaa
      121 tatcccaacc tgcaatcatt cattctgcac taaatccaac ccattattat caacctgtga
      181 acgatgaaac aacagtgatg gaaatcataa aagcatattc aaaataatat taatagatat
      241 aatcctgctt aaggtatagc ttaggcagga ttatatctat tcagctctta ttataaggta
      301 gtgtatacat gaataatctg ccctgtccca ctgacacttt taaatttaat tttatcaaca
      361 ccagcgccct tgcaagagat ctgataagat gctggaccaa taacgttaac tgttcttggc
      421 ccattagggg cataccagat aaaagcaata aatggaccgg gtacattaga caatgtatca
      481 aaatagacag tgtacgcgat atcaccagta tgattttcaa gttcaaccca aggaccaaaa
      541 aagaaaggag gagcccaaac attttgagaa atcatataat ctcctgatta gttcaatgcg
      601 aatgttcttc ccattcactc tcgcaagaaa catctataag ctcaacatct agcccactaa
      661 cagaatcaga gaaatactta taaacatcat cccttgttcc cataaggtct gccgaacatg
      721 atagttcaca accattctta ttcctaacta caaataagaa acggtgaagc aatacttttt
      781 cttctttcat tttatccctc cctttattta cacagaatat actgtatata taaacactag
      841 ttttatatac agtataaatt acgccgcatt atttttcctg tcaatgatat gaattttcat
      901 ttttgtgatc tgaatcaccc ttataaaaat caggaaggga agattcgcag cagaaaaaca
      961 gcaccggata acatcaggag aaaaaacaga gaggagataa cgtgagcaaa acaaaatctg
     1021 gtcgccaccg actgagcaaa acagacaaac gcctgctggc tgcacttatc gctgccggat
     1081 acgaagagcg gacagcccgt gacctcatcc agaaacacgt ttacacactg acacaggccg
     1141 acctgcgcca tctggtcagt gaaatcagta acggtgtggg acagtcacag gcctacgatg
     1201 cgatttacca ggcaagacgc attcgtctcg cccgtaaatt cctgaacgga aaaaaaccgg
     1261 aagaagggct gaacgcctgg aagggtgatt taccataatc acttattgta cgcaccgctg
     1321 aaacgcgttc ggcgcgatca cggcagcaga caggtaaaaa tggcaacaaa ccacccgaaa
     1381 aactgccgcg atcgtacccg gtaaatttta accacatgca tagctatgca gctatgtgaa
     1441 tcatgcagga attgccagcc agagacagct gaaacggatt tttttcatgc tttcggaagc
     1501 gaagaagacg gggaccggag cgggaaaaca gaagttcagg ggaatgagcg cgatctggca
     1561 atagaggcaa aacacagcaa caaaagacac accagaatcg cgcccgtatg cgttttaacg
     1621 cgttcctgtg ttttcagcaa ggtctgactg aagcgcgttc ccggtgcccc cgaaccaacc
     1681 attatcccaa aaaatgcacc ggaaacgcgc tgacaacatt ttagcctgtc tctgattacc
     1741 atcccagcga agggccatcc agtgtccgtt cctgtatttc cagctggcgc tcacgctcca
     1801 gggataacgc atgttcgcgt tcctgtatca gccgttcgtc ccttatctcc tgttctcgct
     1861 gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc tgcacctgct gactcaactg
     1921 catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca ttttctgcaa
     1981 ttccacgaag gcgctctccc attcgttcag ccgctgcata tagtcctgtt gcagttgctc
     2041 taatgcgttc agcaaatgtc tttccagctc cgtcatgctc tgttactcct gtaccagcct
     2101 tacggcattc tgtttcccgt tttccatcgt ccagttcgtt tcggctttca tcccctcggg
     2161 caacaccaga aaacgtccgt tgctcccctc gtgataacgc acgccccatg tcttcgcgtt
     2221 cagcttctcc agattctcgt tctgcttcct gatttcttcc aggttcgcct gtatcatccc
     2281 gccaagatac cagagcgtcc cgccactcac ggtaaacagg aaaatgacca tccccagtaa
     2341 catcatgccc gtattccctg ccagctttaa cacgtccttc ctgtgctgca tcatcgcctc
     2401 tttcacccct tcccggtgtt ttttcagtga ttcctctgtc gaagctgtga acagggctat
     2461 agcgtctttg attttcgtct cgtttgatgt cacagccttg cttacagatt tttcgagctt
     2521 cctgaactcg tcgttcagta ttgtctctgt agattcggct ctctctttca gctttttctc
     2581 gaactccgcg cccgtctgta aaagattgct cataaaatgc tcctttcagc ctgatattct
     2641 tcccaccgtt cgggtctgca atgctgatac tgcttcgcgt caccctgacc acttcaagcc
     2701 ctgcctctgt gagcgcctga atcacatcct gacggccttt tatctccccg acatgataaa
     2761 gagcgtctat cccgcgtgtg acgctctctg caagcgcctg tttcgtttcc ggcaggttat
     2821 cagagagagt cagtatccgg cggttctccg gggcgttggg gtcatgcagc ccgtaatggt
     2881 gattcaccag cgtctgccag gcatcaattc tcggcctgtc tgcccggtcg tagtacggct
     2941 ggaggcgttt tccggtctgt agctccatgt tcggaatgac aaaattcagc tcaagccgtc
     3001 ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcgaga ccgggcatca
     3061 gtacacgctc aaagctcgcc atcacctttt cacgtcctcc cggtggcagc tccttctccg
     3121 cgaacgacag cacaccggac gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc
     3181 gtacttcttc cggattaccc tgaagcaccg ttgccccttc ccggttacgc tcccttccca
     3241 gcaggtaatc aaccggacca ctgccaccac cttttccccg ggcatgaaac ttaactatca
     3301 tcccgcgtcc cctgttccct gacggccaga cgcagccggc gcagctcatc cccgatggcc
     3361 atcagtgcgg ccaccacctg aacccggtca ccggaagacc actgcccgct gttcaccttc
     3421 ctcgctgtct gattcaggtt attcccgatg gcggccagct gacgcagtaa cggcggtgcc
     3481 agtgtcggca gcctcccgga acgggcaacc ggctcaccca ggcagacccg ccgcatccat
     3541 accgccagct gtttaccctc acagcgttca agtaaccggg catgttcatc atcagtaacc
     3601 cgtattgtga gcatcctctc gcgtttcatc ggtatcatta tccccatgaa cagaaatccc
     3661 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     3721 ataagaagcc agacattaac gctgctggag aagctcaacg aactggacgc ggatgaacag
     3781 gcagatatct gtgaatcgct tcacgaccac gccgatgagc tttaccgcag ttgcctcgct
     3841 cgcttcgggg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     3901 tgtctgtgag cggatgccgg gagctgacaa gcccgtcagg gcgcgtcagc aggttttagc
     3961 gggtgtcggg gcgcagccct gacccagtca cgtagcgata gcggagtgta tactggctta
     4021 accatgcggc atcagtgcgg attgtatgaa aagtacgcca tgccgggtgt gaaatgccgc
     4081 acagatgcgt aaggagaaaa tgcacgtcca ggcgcttttc cgcttcctcg ctcactgact
     4141 cgctacgctc ggtcgttcga ctgcggcgag cggtatctac tcactcaaaa acgaaatgct
     4201 gttatccaca gaatcagggg ataacaccgg aaaaaacatg tgagcaaaag accaggaaca
     4261 ggaagaaggc cgcgccgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4321 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg acttaaagat accaggcgtt
     4381 tccccccgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4621 aacgccactg gcagcagcca ttggtaacta aaaagtggat ttagatacgc agaactcttg
     4681 aagttgaagc cttatagcgg ctacactgaa aggacatctt ttggtatctg tgctccactt
     4741 aagccagcta cccggttagg taatctgcca ggattagcta accttcgaaa aaccacctgc
     4801 cagggtggtt ttttcgttta caggcagcag attacgcgca gaaaaaaagg atctcaagaa
     4861 gatcctttga tcttttttct ctaaactgca atcccatcag ttcaggagag aaagatagta
     4921 taattgccct ctcttatatg gaaccaccat tcaatcacat catgggaata atataagtga
     4981 aaacgttcgc attgtttatt tttacgtgta tatgtattta tagattggaa ttttaacaaa
     5041 ataaaatgga gttaatatgt tcaaatcatc agtaaagtca ataacatcga gtactatact
     5101 gaccttactt ataggatgtt caagtactga aacaaacaaa aacattgttt acaaagttgg
     5161 tggtgacagc atcattctga ataaatattg catacttaaa atgtaccctt cccaatcag
//
LOCUS       DAZPAC010000265         5114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZPAC010000265 DAZPAC010000000
VERSION     DAZPAC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.267"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..379
                     /locus_tag="ACHUPM_003955"
     CDS             <1..379
                     /locus_tag="ACHUPM_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871605.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="ACHUPM_003956"
     CDS             complement(575..2125)
                     /locus_tag="ACHUPM_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR0871606.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="ACHUPM_003957"
     CDS             2297..2743
                     /locus_tag="ACHUPM_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR0871607.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="ACHUPM_003958"
     CDS             complement(2937..3872)
                     /locus_tag="ACHUPM_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0871608.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4393..>5114
                     /locus_tag="ACHUPM_003959"
     CDS             4393..>5114
                     /locus_tag="ACHUPM_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871609.1"
                     /translation="MNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMTVGK
                     AFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSIAQN
                     NNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYLDRM
                     LYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKNASP
                     LITKNFPGFYERVPPCENADEC"
BASE COUNT         1417 a         1244 c         1127 g         1326 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attgcaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgccgga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcagag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa ggagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 gcagatgaat gcat
//
LOCUS       DAZPAC010000266         5061 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZPAC010000266 DAZPAC010000000
VERSION     DAZPAC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.268"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            93..1532
                     /gene="norV"
                     /locus_tag="ACHUPM_003960"
     CDS             93..1532
                     /gene="norV"
                     /locus_tag="ACHUPM_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR0871610.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            1529..2662
                     /gene="norW"
                     /locus_tag="ACHUPM_003961"
     CDS             1529..2662
                     /gene="norW"
                     /locus_tag="ACHUPM_003961"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR0871611.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(2790..5042)
                     /gene="hypF"
                     /locus_tag="ACHUPM_003962"
     CDS             complement(2790..5042)
                     /gene="hypF"
                     /locus_tag="ACHUPM_003962"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR0871612.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
BASE COUNT         1222 a         1420 c         1376 g         1043 t
ORIGIN      
        1 tatagagtaa aaacaatcag ataaaaaact ggcacgtaat ctgcaattag caagacatct
       61 ttttagaaca cgctgaataa attgaggttg ctatgtctat tgtggtgaaa aataacattc
      121 attgggttgg tcaacgtgac tgggaagtgc gtgattttca cggtacggaa tataaaacgc
      181 tacgcggcag cagttacaac agctacctca tccgcgaaga aaaaaacgtg ctgatcgaca
      241 ccgtcgacca taaattcagc cgcgaatttg tgcagaacct gcgtaatgaa atcgatctgg
      301 cggatatcga ttacatcgtg attaaccatg cagaagagga ccacgctggg gcgctgaccg
      361 aactgatggc gcaaattccc gatacgccga tttactgtac tgccaacgct atcgactcga
      421 taaatggtca tcatcatcat ccggagtgga attttaatgt ggtgaaaact ggtgacacgc
      481 tggatatcgg caacggcaaa cagctcattt ttgtcgaaac gccaatgctg cactggccgg
      541 acagcatgat gacttacctg acaggcgacg cggtgctgtt cagtaacgat gctttcggtc
      601 agcactactg cgacgagcat ctgttcaacg atgaagtgga tcagacggag cttttcgagc
      661 agtgccagcg ttactacgcc aatatcctga cgccgttcag ccgcctggta acaccgaaaa
      721 ttaccgagat cctgggcttt aacttaccag tcgatatgat agcgacctct cacggcgtgg
      781 tatggcgcga taacccgacg caaattgtcg agctgtacct gaaatgggcg gctgattatc
      841 aggaagacag aatcaccatt ttctacgaca ccatgtcgaa taacacccgc atgatggctg
      901 acgctatcgc ccaggggatt gcggaaaccg acccacgcgt ggcggtgaaa attttcaacg
      961 tcgcccgaag cgataaaaac gaaatcctga ccaatgtctt ccgctcaaaa ggcgtgctgg
     1021 tcggcacttc gacgatgaat aacgtgatga tgccgaaaat cgccggtctg gtggaggaga
     1081 tgaccggatt acgcttccgt aacaaacgcg ccagtgcttt cggctctcac ggctggagcg
     1141 gcggtgcggt ggatcgtctt tccacgcgcc tgcaggatgc gggtttcgaa atgtcgctta
     1201 gcctgaaagc caaatggcga ccagaccagg acgctctgga gttatgccgt gaacatggtc
     1261 gcgaaatcgc ccgtcagtgg gcgctcgcgc cgctgccgca gagcacggtg aatacggtgg
     1321 ttaaagaaga aacctctgcc accacgaagt ccgacctcgg cccacggatg cagtgcagcg
     1381 tctgccagtg gatttacgat ccggcaaaag gcgagccaat gcaggacgtt gcgccaggaa
     1441 cgccgtggag tgaagttccg gataacttcc tctgcccgga atgctctctc ggcaaagacg
     1501 tctttgacga actggcatcg gaggcaaaat gagcaacggc attgtgatca ttggttcggg
     1561 cttcgccgcc cgccaactgg tgaaaaatat tcgcaaacag gacgccagta ttccattaac
     1621 cctgatcgcc gccgacagca tggatgagta caacaaaccg gacctcagcc atgttatcag
     1681 ccaggggcaa cgtgccgacg accttacccg ccagacggcg ggtgaatttg ccgggcagtt
     1741 taatctgcac ctgtttccgc acacctgggt gacggatatc gatgccgaag cccatgtggt
     1801 gaaaagccag aataatcagt ggcaatacga caagctggtg ctggcaactg gtgccagtgc
     1861 ctttgtcccg ccagtgcctg ggcgtgagtt aatgctgacg ttaaatagtc agcaagagta
     1921 tcgcgcctgt gaaacgcaac tgcgggatgc ccgacgcgtg ttgattgtcg gcggtggttt
     1981 gattggtagc gaactggcga tggatttttg tcgtgcaggc aaagcggtca cgctaatcga
     2041 caacgctgcc agtattctgg cgtcattaat gccaccagaa gtaagcagcc gcttgcagca
     2101 tcggttgacg gagatgggcg ttcatctgct gttgaaatct cagttgcagg ggctggaaaa
     2161 aacggattct ggcattctgg caacgctgga ccaccagcgt agtatcgaag tggatgcggt
     2221 aattgccgcc accggactgc gcccggaaac agccctggca cgacgcgccg ggctgacgat
     2281 taatcgcggc gtttgcgtcg atagttatct gcaaaccagt aatgacgata tttatgcgct
     2341 gggcgattgc gcggaaatca acggtcaggt actgccattc ctgcagccga ttcaacttag
     2401 cgcgatggtg ctggcaaaaa atcttctcgg caataacacg ccgctgaaac taccggcgat
     2461 gctggtgaaa atcaaaacgc cggaattacc gctgcatctg gcaggcgaaa cccagcgtca
     2521 ggatttacgc tggcaaatta ataccgaacg ccagggaatg gtggctcgcg gtgttgacga
     2581 tgctgaccag cttcgcgcct ttgtggtcag tgaggatcgg atgaaagagg catttggatt
     2641 gttgaaaaca ttgccgatgt aggtgggcta ctgtgcctaa aatgtcggat gcgacgctgg
     2701 cgcgtcttat ccgacctacg gggacgcatg tgtaggccag ataaggcgtt tacgccgcat
     2761 ccggcaatgg tgtccaaatg caacacgttt tatccgttct ggacttcacc cgctaaccaa
     2821 cgcgccgcag caataacccc ctgccccaga gacaaaccgc catcacccgc cggtaaactc
     2881 tgtggaaaga gcaatgtgaa atcagcgaga taatgcgcca gacgtgcacg cagcaaacgg
     2941 ttatgaataa ccccgccgct aaataccagc gtagtgatac cacgcatcgt ggcctgctca
     3001 cgcatcaacg cggcaaaacc ctgcgccagc gcatcatgaa acgcccacgc gcgttgatta
     3061 accggtgcct gccagttcag ccactgctgc cagaaagcgg cgagatccag ttgattgtcc
     3121 accagcggca tcgtcaccgg atgcgtcact ccgtggcatg aggctgcgag cgcctccaga
     3181 gcacaagccg cttcaccttc ataacttaac gtggctggcg cacagcccag tgccgccgcc
     3241 actgcatcga acaaccgccc acacgatgac gccagcggcg cgttaattcc acgctcaatg
     3301 gcccgcgcca gcacgctcca gttttgctgt tgcacacttg ctgtttcagg gtaattctgc
     3361 cactccggca caaagcgcag gcactgcgcc agcaggtttc gccacggctg cttcgctgcc
     3421 aaatcgccac ccggaagcgc cactgcaggc aagccgccca ggtgctcaca ttcgcgatag
     3481 ttcacccgca ggcactcgcc gccccacaaa gcgccgttct cccccatacc gataccgtcg
     3541 agcgtcaaag caatgacatc accgccatcc aacggccact gatgctctgc cagacacgcc
     3601 gctgcatggg catgatgatg cagtaccgtt tgcgtcggca gattcatttc acgcgcccac
     3661 tggctggaga catagcccgg atgcgcgtca tgcacaacgt attgcggggt aaaatcgtag
     3721 atgttttgca tcaggcgtaa cgcttcgcgc cactgcatct ggatgccatc gtcacttaaa
     3781 tcgcccagat gctgactcaa caccgcttgt tcaccgcgca ccaggcagaa ggtatttttc
     3841 agatccgcgc cgagacacag cacaggcgga acatttttaa agcccggagg caaagccagc
     3901 gcatccggca catacccccg cgaacggcgc agcatttcgc cgctttcgcg caccaccgaa
     3961 tcatccatcc gctgcacgat gtcgcggtta tgtatcaaga atccgtcggc aatgccctgc
     4021 aaatccgcca gcgcctgttc gttgctgata gctggtggtt taccgctcag gttgccggag
     4081 gtcatcacca gcgggcattg cagttcctgt aacagcaaat gctggagcgg gttcgcaggc
     4141 aacattaccc cgacttcgtt aaggtcaggg gcgatatcat cacaaagctc aggaacgtat
     4201 tttttatcca ccagcacaat cggcgcggcg ggcgtggtaa gcaactggcg cgcagcgtct
     4261 ggtaaaccgt cagccactgg caacatgacc gccagcggtt tcgccgggcg atgtttgcgc
     4321 gcccgaagtg tcgccaccgc gttactgtta cgtgcatcgc aggcaagatg aaatccgcca
     4381 atccctttga tggcgacaat gttgcccatt tttaactgtg cgatagccgc ctgtaatgcc
     4441 gcctcttgtt ctgcatgttc accatgactt acccattcaa gatgtgggcc acactccggg
     4501 caggccaccg gctgggcgtg gaagcgacga tcgagcgggt cacggtactc tttgtcacaa
     4561 gccggacaca acggaaacgc cgccatcacg gtaaacgggc ggtcgtaagg catggcgcga
     4621 ataatggtga aacgcgggcc gcagtgggta cagttgataa acggataacg ataacgccgt
     4681 tcgcctgggg tattcatttc ggcaaggcaa gcagggcaag tagcggcatc cgggacaatt
     4741 tgcgtattca tggcacctcc cgcgctctgg cggatggtga actcagtggg cagttgtgac
     4801 cagatgtacg gctcacgctc gacgctatca atacgcgcca gcggcgggca gtgctgatgc
     4861 aattgaacaa gtaacgtttc cgggtcttcc cgcagccgga cttctacgcc atcgccgtca
     4921 ttacagacat cgccgtgaag atttaattgc tgtgccagct gccagacaaa cggacgaaaa
     4981 ccgacgccct gcactttgcc acgaatacgc agttggacac cgcaagatgt gttttttgcc
     5041 attgagttat tcccgccatc a
//
LOCUS       DAZPAC010000267         5044 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZPAC010000267 DAZPAC010000000
VERSION     DAZPAC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.269"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            73..1601
                     /gene="lsrA"
                     /locus_tag="ACHUPM_003963"
                     /pseudo
     CDS             73..1601
                     /gene="lsrA"
                     /locus_tag="ACHUPM_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1595..1771
                     /locus_tag="ACHUPM_003964"
                     /pseudo
     CDS             1595..1771
                     /locus_tag="ACHUPM_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(1779..1868)
                     /locus_tag="ACHUPM_003965"
                     /pseudo
     CDS             complement(1779..1868)
                     /locus_tag="ACHUPM_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="ACHUPM_003966"
     CDS             complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="ACHUPM_003966"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR0871613.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3750..4700)
                     /locus_tag="ACHUPM_003967"
     CDS             complement(3750..4700)
                     /locus_tag="ACHUPM_003967"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0871614.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4839..>5044)
                     /locus_tag="ACHUPM_003968"
     CDS             complement(4839..>5044)
                     /locus_tag="ACHUPM_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HFR0871615.1"
                     /translation="ALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSL
                     SEEQQRYIVAVIYHWLVVQMNQP"
BASE COUNT         1316 a         1288 c         1230 g         1210 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgtcgattt tacgttgcat caaggggagg
      181 tccacgccct gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg
      241 gtattacccc tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa
      301 cgccagttca tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc
      361 caagcctgtc gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc
      421 agaaaatgaa gaacttgctg gcggcgctgg gctgctagtt tgatctgcat agtctggcag
      481 gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct ccgcggcctg atgcgcgact
      541 cgcggattct gatcctcgat gaacctaccg cctcgcttac ccctgcggaa accgaacgct
      601 tgtttcgtcg cttgcaagag ctgcttgcta ctggcgtggg tattgttttt atctcgcata
      661 agctgccgga aattcgccag attgccgatc gaattagcgt gatgcgcgac ggaaccatcg
      721 ccttaagcgg caaaaccagc gaactgtcta ccgatgacat tattcaggcc atcacgccag
      781 tggtacggga aaaatcgctc tctgccagcc aaaaattatg gctggagtta cctggcaacc
      841 gcccacaaca tgccgccgga acgtcggtgc tgacactaga aaatctgacc ggcgaaggtt
      901 tcaggaatgt cagcctgacg ctcaatgccg gagaaattct gggtctggcc gggctggtgg
      961 gggccggacg cacagaactg gccgagacgc tctatggtct gcgtactttg cgtggcggac
     1021 gcattatgct gaatggtaaa gagatcaata aattatccac tggagaacgt ttactgcgcg
     1081 gtctggttta tctgccggaa gatcgccagt catccggact gaatctcgat gcttcgctgg
     1141 cctggaacgt ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa accgcgaaag
     1201 ataatgccac cctggaacgt tatcgtcggg cgctgaatat taaatttaac caaccggaac
     1261 aagctgcacg gacattatcc ggtggcaacc agcaaaaaat cctgattgcc aaatgcctgg
     1321 aagcttcgcc gcaagcattg attgtcgatg agccgacgcg cggcgtggat gtctcggccc
     1381 gtaatgatat ctaccagctg ttgcgcagca tcgccgcaca gaatgtggca gtgctgctta
     1441 tctcctctga tctggaagag atcgaactga tggcagatcg tgtgtatgtg atgcatcagg
     1501 gcgaaattac ccactctgca ctgaccgggc gcgatattaa tgtcgagact attatgcgcg
     1561 ttgccttcgg cgatagtcag cgtcaggagg cgtcatgctg aagtttattc agaacaaccg
     1621 tgaaatcacg gcactgctgg cggtggtgct gctgtttgta ttacccggtt ttctcgaccg
     1681 ccagtattta agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct
     1741 gctggcaatg ggcgcgacgc tggtaatgct tatgcgcaaa gtagtaagcg cgatgatatc
     1801 ttggataata ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga
     1861 acaatcatgg ttatcttcag gcgtgaaaaa gtcgccctat taaacgtgat tacgatcaca
     1921 ttctcaagac gctacttaca aattacctac aagcttacaa cattaacagg cgctgcatgt
     1981 ggcatctgtt aatgataaag gcacatagga tgttgtaact aatatagtga aataagaaac
     2041 ccggtcaaaa ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc
     2101 gcatcccttt ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca
     2161 cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct
     2221 gggtaccgat aacatggtca cgatgctggc tccagagttg ctgataacgt tccagccagt
     2281 gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag aacgcaatca
     2341 atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca
     2401 gcagctgcgg caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca
     2461 gctgatgctt atacgggtta cggaaacgcc cggtgactgc actggcgaaa gattccagtt
     2521 catcacgagg caaatccagt accggaataa tttcttcgta gatggctttt tcaacgaatg
     2581 cgcaaatttc agcatcgttc atcgcttcac ctacggtatc cagccctgcc tggaacgcca
     2641 ccggcaccag cgcggtgtgc gcaccgttga ggattgccac tttgcgttct ttatacggtt
     2701 taatatcgtc aacaatcagc acgttgagcg gatatttgtc caggcgcagc tcagtcgcta
     2761 aggatttcgg cccctgaatc acaaacaggt aaaagtgttc agcggtgtcg aggaaaccat
     2821 cgtgataacc cagttcttct tccagtttag ccacttcatc gcgcggataa ccggtaacga
     2881 tacggtccac cagcgtagaa cagaagctgt tagcctgatc cagccattga ataaatgctt
     2941 ctggcagtgc ccactcttgc gcatagcgca gcaccagttc acgcaacgcg tcgccattgt
     3001 agtcgatcaa ctcacacggg atgatgatcc aacctttatc cagcgcaccg ttgaaatggc
     3061 taaagcgttc gaacagcaga cgggtcagtt ttgccggata gcttatcgct ggcgcgtcat
     3121 cgaatttatc gcccgcatgg tagctgatcc ccgcttcggt ggtgttagag aaaacaaagc
     3181 gcatttccgg gttgtgcgcc agtttcagga attcatcgta ttcactgtag acgctgattt
     3241 cacgattaac cgagcgaatc agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc
     3301 cacggataat ggtggtatac agaccatcct gcgtgctcag tgacggcggg aatgaagttt
     3361 caatcggacg aacaacgacc acgccagaat tcagatcggt gtgctcattc aggagatcga
     3421 tttgccagtc aacaaaggcg cgcaggaagt taccttcacc aaactgaatg atacgttctg
     3481 gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt tcccttctga
     3541 ttagtcatac aacctgtttg aattggtacg acaggttagc aaactttaat acgccgaacc
     3601 cctgttttga tcaactcctg atgattaatg agcagtttta tgagaaaagt gtggcgcgga
     3661 tcatggttta atcgaggaaa tacgcctttt cctggatcat aaagtggtag aacacattgc
     3721 attcaaatcg cgcggaatga ataaagatgt cagacaactt cctcaccgta acgcatagtg
     3781 ctggtacggt tgcgcccatc tttcttcgag cgatacagac acgtatcggc ttcgaccatc
     3841 aatttattaa aatcatcggt cagcgttcgg tacgatgcgc gaccactacc gacgccaata
     3901 cttaccgtga gataaagcgt tttttgttgc caggtgaatg gttgcagttc aacgccttta
     3961 cggatttttt ccgccattag cagaccatcg acaggattca ccgacggcac tgcaacagca
     4021 aattcttcgc cgcccatgcg cgccaccagc cccttatcac cgacaatctt ctgaatatgc
     4081 cgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc gttaatgctt
     4141 ttgaaatagt cgatatcaag cagcatgacc gtcagaggca cgatcaacac gcccgttgag
     4201 aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc aatactgacc
     4261 gaaccaatcg ccccgacaat agcgcataat aatgcggcaa tcagacagta attaaatagc
     4321 cgtaaggcac ttactggctc atacttattt ttccccagac gcttatcccg cacgaccagt
     4381 aaggcgacgg taacaataaa catcatattg gagaaattga taaccagtga aaccagtccc
     4441 cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc atagtaatgc
     4501 agtcgattaa gccagacata gcgggcaaac ccccccgcca ttacgccatt cagcggccag
     4561 aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt
     4621 agcgcaaaaa tagcgatgct attacgtagc agatgacctt cctggaacaa acggaaagtt
     4681 gaaatggggt gtacatgcat taattatctg tccatacaat tgcgggtggc aattatctca
     4741 acgtaataag taaattctca taaaggatag catgttcgct aacagcggcg gcaaataaag
     4801 gtaccctggg aatgctattt tatcgttttc cgcaattctc atggctgatt catctgtaca
     4861 accagccagt gatagattac cgccacgata taacgttgtt gttcttcgct taacgaaaca
     4921 ccctgcggaa tgttatgcca cttagccaga caaccgcgac agcaggtcgc ggtggcgtgc
     4981 tgagcgataa acaccggatg cccgcgcatg ggggtttgct taccgtcatt agccggtaat
     5041 gccg
//
LOCUS       DAZPAC010000268         4997 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZPAC010000268 DAZPAC010000000
VERSION     DAZPAC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.270"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..164)
                     /locus_tag="ACHUPM_003969"
     CDS             complement(<1..164)
                     /locus_tag="ACHUPM_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR0871616.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFA"
     gene            complement(383..1360)
                     /gene="epmA"
                     /locus_tag="ACHUPM_003970"
     CDS             complement(383..1360)
                     /gene="epmA"
                     /locus_tag="ACHUPM_003970"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR0871617.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1685..3493
                     /gene="frdA"
                     /locus_tag="ACHUPM_003971"
     CDS             1685..3493
                     /gene="frdA"
                     /locus_tag="ACHUPM_003971"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR0871618.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3486..4220
                     /gene="frdB"
                     /locus_tag="ACHUPM_003972"
     CDS             3486..4220
                     /gene="frdB"
                     /locus_tag="ACHUPM_003972"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR0871619.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4231..4626
                     /gene="frdC"
                     /locus_tag="ACHUPM_003973"
     CDS             4231..4626
                     /gene="frdC"
                     /locus_tag="ACHUPM_003973"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR0871620.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4637..4996
                     /gene="frdD"
                     /locus_tag="ACHUPM_003974"
     CDS             4637..4996
                     /gene="frdD"
                     /locus_tag="ACHUPM_003974"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR0871621.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
BASE COUNT         1254 a         1293 c         1311 g         1139 t
ORIGIN      
        1 aaggcgaatg gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa
       61 cccattaagt aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc
      121 aatatgagtc ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt
      181 tcagcgcgtg tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt
      241 aaaaaccaat acagaattga tacccggatg atatcaaata tccagtctta aattgtggcc
      301 tttaacggaa aaatgaaaac atatgctaca gaatggcgct tatcacgcca ttcttcgctg
      361 ttaattcagt aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca
      421 gtgtctccgc gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac
      481 agtcaggcat accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca
      541 aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat
      601 ccgttaattc atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt
      661 cagcgacccg atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt
      721 ggtacacaaa ggtcggtttt tctttgccaa tatttggctc tacgccaaag gtaaacagca
      781 attgtagcaa cgtgtcgcgg tcttcttcgg tatcagcaac gttactcaaa tccagtttcg
      841 ctgcgacttc ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca
      901 agaaagcttg ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga
      961 gatcgtccac ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactcaagca
     1021 tagtgaactc agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct
     1081 ggaataccgg cccacaaccg gcaaccagca ggcgtttcat atggtattcc gggctggtca
     1141 ttaaccagag attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga
     1201 ccaaatgaat atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc
     1261 cacgatcggc aaagaaacga cggatctccg ccataatcgc cgcacgtttt aataagttag
     1321 gaatggatgc gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg
     1381 acaagggcac gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa
     1441 cagcggtggg cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat
     1501 cttcagtgat aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac
     1561 ttatccatca gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc
     1621 gtaactttca ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa
     1681 tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg
     1741 tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt
     1801 atacccgatg cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga
     1861 tcatgacagc ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga
     1921 gcaggatgtc gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact
     1981 gtgggggtgc ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg
     2041 catgaaaatc gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac
     2101 gctgttccag acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct
     2161 ggatattctg gttgatgatg gtcatgttcg cggcctggta gcaatgaaca tgatggaagg
     2221 cacgctggtg cagatccgtg ctaacgcggt cgttatggct actggcggtg cgggtcgcgt
     2281 ttatcgttac aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag
     2341 ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg
     2401 ttccggtatc ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa
     2461 tggctaccgt tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa
     2521 aaacaaatat atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg
     2581 gcgtaaaggc aacaccatct ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca
     2641 cctcggcgag aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta
     2701 cgttggcgtc gatccggtta aagaaccgat tccggtacgt ccgaccgcac actacaccat
     2761 gggcggtatc gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg
     2821 tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga
     2881 actggtggtc ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg
     2941 taatggcaac gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga
     3001 tctggttaac caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc
     3061 aatggaagaa ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa
     3121 gctggcagag ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt
     3181 caacaccgac ctgctctaca ccattgaact gggccacggt ctgaacgttg ctgaatgtat
     3241 ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg
     3301 ttgcaccgag cgtgacgacg tcaacttcct caaacacacc ctcgccttcc gcgatgctga
     3361 tggcacgact cgcctagagt acagcgacgt gaagattact acgctgccgc cagctaaacg
     3421 cgtttacggt ggcgaagcgg atgcagccga taaggcggaa gcagccaata agaaggagaa
     3481 ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg
     3541 ataccgcacc gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg
     3601 atgcgctggt ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc
     3661 gtatggcgat ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta
     3721 aaaccttcct gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga
     3781 ttgaacgcga tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac
     3841 cgtacatcat cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc
     3901 agatggcaaa gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt
     3961 gcccgcagtt tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc
     4021 gttataacga agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc
     4081 agaacggcgt atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg
     4141 tcgatccggc tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg
     4201 cgaccctgaa accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg
     4261 ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa
     4321 ggcacggcgg ttccggctgt gtggttcagc attgaactga ttttcgggct gtttgccctg
     4381 aaaaatggcc cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg
     4441 atcattaacc tgatcactct ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg
     4501 gcaccgaaag cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc
     4561 aaaagtctct gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac
     4621 tggtaaggag cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg
     4681 gggcctcttc ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct
     4741 ggtgggtatt ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt
     4801 tctggcgttc gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc
     4861 gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc
     4921 tgcgggcaaa tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg
     4981 tatcgttaca atctaac
//
LOCUS       DAZPAC010000269         4957 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZPAC010000269 DAZPAC010000000
VERSION     DAZPAC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.271"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..112
                     /locus_tag="ACHUPM_003975"
     CDS             <1..112
                     /locus_tag="ACHUPM_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR0871622.1"
                     /translation="AAKVLKQVMVNTGGNKEQVPAKRQNRGSRNSTDDGG"
     gene            138..659
                     /locus_tag="ACHUPM_003976"
     CDS             138..659
                     /locus_tag="ACHUPM_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR0871623.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDKGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            715..948
                     /locus_tag="ACHUPM_003977"
     CDS             715..948
                     /locus_tag="ACHUPM_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFR0871624.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            1012..2970
                     /locus_tag="ACHUPM_003978"
     CDS             1012..2970
                     /locus_tag="ACHUPM_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR0871625.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            3025..3459
                     /gene="psiB"
                     /locus_tag="ACHUPM_003979"
     CDS             3025..3459
                     /gene="psiB"
                     /locus_tag="ACHUPM_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR0871626.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3456..4218
                     /locus_tag="ACHUPM_003980"
                     /pseudo
     CDS             3456..4218
                     /locus_tag="ACHUPM_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            4397..4546
                     /gene="mok"
                     /locus_tag="ACHUPM_003981"
                     /pseudo
     CDS             4397..4546
                     /gene="mok"
                     /locus_tag="ACHUPM_003981"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            4488..4613
                     /locus_tag="ACHUPM_003982"
     CDS             4488..4613
                     /locus_tag="ACHUPM_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFR0871627.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
BASE COUNT         1135 a         1289 c         1543 g          990 t
ORIGIN      
        1 tgcggcgaag gttctcaaac aggtgatggt gaataccgga gggaacaaag agcaggtccc
       61 cgcaaaacgt caaaaccgcg gctcccgaaa ctcaacagat gatgggggct gaaaacatca
      121 acagaaggag acacatcatg gcagttcgtg gcattaacaa ggtcattctt gtcggtcgtc
      181 tgggaaaaga tccggaagtc cgttacatcc ccaacggggg cgcagtggca aacctgcagg
      241 tggccacgtc agaaagctgg cgtgacaaac agacggggga gatgcgggag cagacagaat
      301 ggcatcgcgt ggtgctgttc ggcaagctcg cggaagtggc aggtgaatat ctgcgcaagg
      361 gcgcgcaggt ctacatcgag gggcaactcc gaacccgtag ctgggaagat aaaggtatta
      421 cccgttacgt cactgaaatc cttgtgaaaa ccacgggcac catgcagatg ctggggagtg
      481 caccacagca gaacgctcag gcgcaaccgc agcctcagca gaatggtcag ccacagagtg
      541 ctgacacgac gaaaaaaggg agcgcgaaaa caaaaggccg tggacgtaag gccgcgcagc
      601 cggagcctca gcagccgtct gaacccgcgt atgacttcga tgacgacatt ccgttctaac
      661 gaaagacagt gacaaccgcc ccgtccagtg cggggcatca ccggagatat gaggatgagc
      721 gaatatttca gaatatttca ggggctgcct gacggctcct ttacccgcga acaggctgaa
      781 gccgttgccg cacagtaccg gaatgtcttt atcgaggatg atcagggaac gcattttcgc
      841 ctggttgtcc gcaataacag tggcctggtc tggcgtacct ggaattttga ggacggtgcc
      901 gggtactgga tgaaccatgt catccggaat ttcggcattc ttaagtaaga gaggtgccgg
      961 acgggaacgc gtccggcaga catacaacca tcccggtcaa aggagaaata tatgtcagtt
     1021 acagagtcta agacaaaaac ggagcgtaaa tccagccgta aacctgcaaa aacgcaggaa
     1081 acagtcctgt ctgccctgct ggagcagacg gaggaagtga gcgtgtcgct ggcctcgctg
     1141 attaaatcac cgctgaatgt gcgcacggtg ccgtattccg cagagtccgt cagcgaactg
     1201 gcggagtcca ttaagggcgt tggcctgttg cagaatctgg ttgttcatgc cctgcctggt
     1261 gaccgccacg gtgtcgccgc aggtggtcgc cgactggcag cactcaacat gctggcagag
     1321 cgtggcatcc ttccggctga ctggcctgta cgcgtgaagg ttattccgca ggagctggcg
     1381 acggctgcat caatgactga aaacggtcag cgtcgggata tgcaccctgc cgaacagatt
     1441 gccggattcc gcgcaatggc gcaggaaggc aaaacacctg cacaaatcgg tgatttgctg
     1501 ggctattcac cccgccacgt tcagcgaatg ctgaaactgg ctgaccttgc gcctgtcatc
     1561 ctcgatgcgc tggcagaaga ccgcatcacc accgaacact gtcaggcgct ggcgctggag
     1621 aacgacaccg cgcgtcaggt gcaggtgttt gaagccgcct gtcagtcggg atggggcggt
     1681 aaaccggaag tacagaccat tcgtcgtctg gtgaccgaaa gtgaagtggc ggtggcgggg
     1741 aacagtaaat tccgcttcgt gggggctgat gccttctcgc cagacgaact gcgcaccgat
     1801 ttgttcagcg acgacgaagg tggctatgtg gactgcgtgg cgctcgatgc cgccctgctg
     1861 gaaaaactcc aggctgtcgc agagttcctt cgggaagccg aaggctggga atggtgcgcc
     1921 ggacgcatgg agcctgtcgg tgagtgccgt gaggatgccg gaacataccg ctgtctgccg
     1981 gagccggaag cggtgctgac ggaggcggaa gaagaacgtc tgaacgaact gatgacgcgt
     2041 tacgacgcgc tggaaaacca gtgtgaggaa tctgacctgc tggaagcaga aatgaagctg
     2101 atgcgctgca tggcgaaggt cagggcgtgg acgccggaga tgcgtgccgg aggtggagtg
     2161 gtggtgtcct ggcgttatgg caacgtgtgt gtccagcgtg gtgtgcagtt gcgcagtgag
     2221 gatgacgtgg ctgacgacgc tgaccgtacg gaacaggtgc aggagaaagc gtcagtggag
     2281 gaaatcagtc tgccactgct gacgaaaatg tcttccgaac gcacgctggc agtccaggca
     2341 gcactgatgc agcaaccgga caaatccctg acactgctgg catggacgct ctgcctgaat
     2401 gtgtttggca gcggagcgta cagtaaacca gcacaaatca gcctggaatg taaacattat
     2461 tcgctgacca gcgatgcgcc atcggggaag gaaggtgccg cattcatggc gctgatggca
     2521 gaaaaagccc gtcttgctgc cctgttaccg gagggatggt cacgggacat gacgacattc
     2581 ctgtcactca gtcaggaggt gctgttatcc ctgctcagtt tctgcaccgc atgcagcctt
     2641 aacggtgtcc agacccgtga gtgtggtcac acgtcacgca gtccgcttga cacgctggaa
     2701 agcgccatcg gctttcacat gcgcgactgg tggcagccga caaaagcaaa cttcttcgga
     2761 cacctgaaaa agccgcagat tatcgcagcc ctgaatgagg cagggctgtc cggtgccgca
     2821 cgggacgcgg agaagatgaa gaaaggtgat gcggctgaac atgcagagca ccatatgaaa
     2881 gacaaccgct gggtgcctgg ctggatgtgt gcaccacatc cacagacaga taccactgaa
     2941 cgcaccgata acctggctga tgccgcctga tgaacaacca caccgccccg ccggagacgg
     3001 ggcggcagca agggagatac cgtgatgaaa actgaactga ccctgaatgt attacagacc
     3061 atgagcgcac aggaatatga agatatccgg gctgccggaa gtgatgaacg tcgtgagctg
     3121 acacacgccg tgatgcggga actggatgcg ccggacaact ggacgatgaa cggcgagtac
     3181 ggcagcgagt ttggcggatt tttccccgtc caggtccgtt tcacgcctgc ccacgaacgt
     3241 tttcacctgg cgttatgttc accgggcgat gtctctcagg tctgggtgct ggttctggtg
     3301 aatgccggtg gtgagccgtt tgcggtggtt caggtacagc ggcggtttgc accggaagcc
     3361 gtcagccatt cactggcact ggcggcgtca ctggacgcgc aggggtacag cgtcagtgac
     3421 atcatccata tcctgatggc ggaaggaggt caggcatgag cgcacgttca caggcactgg
     3481 ttcccctcag cgcagagcaa caggccgcat ggcgggcggt ggcggagaca gaaaaacgcc
     3541 gccaccaggg aaacacgctg gcggaatatc cgtatgcggg ggcgtttttc cgctgtctga
     3601 acggaagccg caggatatcg ctgtctgacc tgcgattttt catgccgtcc ctgaccgcag
     3661 aagaactgca cggcagccgt cttcagtggc tgtatgccat cgatgtgctg attgaaacac
     3721 agggcgaggt ctgcctcctt cctcttccag gcgatgcagc agaacgcttg ttcccgtcgg
     3781 tgcggtttcg tgtcagggaa cgcagtcgcc ataaatccgc gctggtcatg cagaagtaca
     3841 gccgccagca ggcgagggag gcagagcaga aagcccgtgc gtaccaggcg ctggtcgctc
     3901 aggctgaaat cgaactggca ttccattcgc cggaaacagt cggaagctgg tatgcacgct
     3961 ggagtgaccg ggtggcagag catgacctgg aaaccctgtt ctggcagtgg ggtgagcgtt
     4021 ttccgtcact ggccggaatg gaacgctggc agtggcagga tatgccgttc tggcaggtta
     4081 ttgcggaagc cagtcttgca gcaagagagg ccggtcacgc tgtccgtgag atggagcggt
     4141 ggatggtgcc gaacaaactc cgggaggcgg cgtgatgcgg caacaatcac acagattacc
     4201 cgtaaacagc ctgaatgagc gggttatttt caggaaaagt gagtgtggtc agcgtgcagg
     4261 gatatgggct atgatgtgcc cggcgcttga ggctttctgc ctcatgacgt gaaggtggtt
     4321 tgttaccgtg ttgtgtggca gaaagaagat agccccgtag taagttaatt ttcattaacc
     4381 accacgaggc atccctatgt ctagtccaca tcaggatagc ctcttaccgc gctttgcgca
     4441 aggagaagaa ggccatgaaa ctaccacgaa gttcccttgt ctggtgtgtg ttgatcgtgt
     4501 gtctcacact gttgatattc acttatctga cacgaaaatc gctgtgcgag attcgttaca
     4561 gagacggaca cagggaggtg gcggctttca tggcttacga atccggtaag tagcaacctg
     4621 gaggcgggcg caggcccgcc ttttcagggc tgatgctggt ctgactgcac tgaagcgcct
     4681 ttataaaggg gctgctggtt cgccggtggc ccctttctcc ttgctggcat tgtacgggca
     4741 tgaacaacca gactgccagg aaggccgttt ccctccggga acggggatga acgtgcgcct
     4801 gcggcgcccg ttcttttccc ccgccttctc tgtttatgac ttaccagaaa tcacatcacc
     4861 ttccctttga ttattctctt tttcctcctg ctctgactct gattactgct ctgactctga
     4921 ttactgctgc cctgattatt gtctgttttg ttgttct
//
LOCUS       DAZPAC010000270         4947 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZPAC010000270 DAZPAC010000000
VERSION     DAZPAC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.272"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            243..1187
                     /gene="leuO"
                     /locus_tag="ACHUPM_003983"
     CDS             243..1187
                     /gene="leuO"
                     /locus_tag="ACHUPM_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR0871628.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1342..1386)
                     /locus_tag="ACHUPM_003984"
     CDS             complement(1342..1386)
                     /locus_tag="ACHUPM_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871629.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1505..3229
                     /gene="ilvI"
                     /locus_tag="ACHUPM_003985"
     CDS             1505..3229
                     /gene="ilvI"
                     /locus_tag="ACHUPM_003985"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR0871630.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3232..3723
                     /gene="ilvN"
                     /locus_tag="ACHUPM_003986"
     CDS             3232..3723
                     /gene="ilvN"
                     /locus_tag="ACHUPM_003986"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0871631.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3735..3821
                     /locus_tag="ACHUPM_003987"
     CDS             3735..3821
                     /locus_tag="ACHUPM_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR0871632.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3903..4907
                     /gene="cra"
                     /locus_tag="ACHUPM_003988"
     CDS             3903..4907
                     /gene="cra"
                     /locus_tag="ACHUPM_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR0871633.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1202 a         1121 c         1304 g         1320 t
ORIGIN      
        1 ttcgcggtag ttatgattag attgttttcg caacaaaaac attatggatt cttatgctgt
       61 ggtaaatgac tcattccacg gcaatggatt ctgtttttat cagaacccgt atctttatgt
      121 tttccgaatt ttactcattt tgctttttct tattttatat gcatgataaa tcatattctt
      181 caggattatt tctctgcatt ccaataaggg aaagggagtt aagtgtgaca gtggagttaa
      241 gtatgccaga ggtacaaaca gatcattcag agacggcgga gttaagcaag ccacagctac
      301 gcatggtcga tctcaactta ttaaccgttt tcgatgccgt gatgcaggag caaaacatta
      361 cccgtgccgc tcatgttctg ggtatgtcgc aacctgcggt cagtaacgct gttgcacgcc
      421 tgaaggtgat gtttaatgac gagctttttg ttcgttatgg ccgtggtatt caaccgactg
      481 ctcgcgcatt tcaacttttt ggctcagttc gtcaggcatt gcaactagta caaaatgaat
      541 tgcctggttc aggttttgaa cccgcgagca gtgaacgtgt atttcatctt tgtgtttgca
      601 gcccgttaga cagcattctg acctcgcaga tttataatca cattgagcag attgcgccaa
      661 atatacatgt tatgttcaag tcttcattaa atcagaacac tgaacatcag ctgcgttatc
      721 aggaaacgga gtttgtgatt agttatgaag acttccatcg tcctgaattt accagcgtgc
      781 cattatttaa agatgaaatg gtgctggtag ccagcaaaaa tcatccaaca attaagggcc
      841 cgttactgaa acatgatgtt tataacgaac aacatgcggc ggtttcgctc gatcgtttcg
      901 cgtcatttag tcaaccttgg tatgacacgg tagataagca agccagtatc gcgtatcagg
      961 gcatggcaat gatgagcgta cttagcgtgg tgtcgcaaac gcatttggtc gctattgcgc
     1021 cgcgttggct ggctgaagag ttcgctgaat ccttagaatt acaggtatta ccgctgccgt
     1081 taaaacaaaa tagcagaacc tgttatctct cctggcatga agctgccggg cgcgataaag
     1141 gccatcagtg gatggaagag caattagtct caatttgcaa acgctaactg aatgcagaat
     1201 aggtcagaca tgaatgtctg gtttattctg tattttttat tgaatgtaga attttattct
     1261 gaatgtgtgg gctctctatt ttaggattaa ttaaaaaaat agagaaattg ctgtaagttg
     1321 tgggattcag ccgatttatt atcaatttaa tccttcgtaa tggaggattt tatcgtttct
     1381 tttcaccttt cctcctgttt attcttatta ccctgcgttt atgtctctgg ctgccaattg
     1441 cttaagcaag atcggacggt taatgtgttt tacacatttt ttccgtcaaa cagtgaggca
     1501 ggccatggag atgttgtctg gagccgagat ggtcgtccga tcgcttatcg atcagggcgt
     1561 taaacaagta ttcggttatc ccggaggcgc agtccttgat atttacgatg cattgcatac
     1621 cgtgggtggt atcgatcatg ttttagttcg tcatgagcag gcggcagtgc atatggccga
     1681 tggtctggcg cgcgcgaccg gggaagtcgg cgtcgtgctg gtaacgtcgg gtccaggggc
     1741 gaccaatgcg attactggca tcgccaccgc ttatatggat tccattccat tagttgtcct
     1801 ttccgggcag gtagcgacct cgttgatagg ttacgatgcc tttcaggagt gcgacatggt
     1861 ggggatttcg cgaccagtgg ttaaacacag ttttctggtt aagcaaacgg aagacattcc
     1921 gcaggtgctg aaaaaggctt tctggctggc ggcaagtggt cgtcctggac cagtagtcgt
     1981 tgatttaccg aaagatattc ttaatccggc gaacaaatta ccctatgtct ggccggagtc
     2041 ggtcagtatg cgttcttaca atcccactac taccggacat aaagggcaaa ttaagcgcgc
     2101 tctgcaaacg ctggtagcgg caaaaaaacc ggttgtctac gtaggcggtg gggcaatcac
     2161 ggcgggctgc catcagcagt tgaaagaaac ggtggaggcg ttgaatctgc ccgttgtttc
     2221 ctcattgatg gggctgggag cgtttccggc aacgcatcgt caggcactgg gcatgctggg
     2281 aatgcacggt acctacgaag ccaatatgac gatgcataac gcggatgtga ttttcgccgt
     2341 cggggtacga tttgatgacc gaacgacgaa caatctggca aagtactgcc caaatgccac
     2401 tgttctgcat atcgatattg atcctacttc catttctaaa accgtgactg cggatatccc
     2461 gattgtgggg gatgctcgcc aggtcctcga acaaatgctt gaactcttgt cgcaagaatc
     2521 cgcccatcaa ccactggatg agatccgcga ctggtggcag caaattgaac agtggcgcgc
     2581 tcgtcagtgc ctgaaatatg acactcacag tgaaaagatt aaaccgcagg cggtgatcga
     2641 gactctttgg cggttgacga agggagacgc ttacgtgacg tccgatgtcg ggcagcacca
     2701 gatgtttgct gcactttatt atccattcga caaaccgcgt cgctggatca attccggtgg
     2761 cctcggcacg atgggttttg gtttacctgc ggcactgggc gtcaaaatgg cgttgccaga
     2821 agaaaccgtg gtttgcgtca ctggcgacgg cagtattcag atgaacatcc aggaactgtc
     2881 taccgcgttg caatacgagt tgcccgtact ggtggtgaat ctcaataacc gctatctggg
     2941 gatggtgaag cagtggcagg acatgatcta ttccggccgt cattcacaat cttatatgca
     3001 atcgctaccc gatttcgtcc gtctggcgga agcctatggt cacgtcggga tccagatttc
     3061 tcatccgcat gagctggaaa gcaaacttag cgaggcgctg gaacaggtgc acaataatcg
     3121 cctggtgttt gttgatgtta ccgtcgatgg cagcgagcac gtctacccga tgcagattcg
     3181 cgggggcgga atggatgaaa tgtggttaag caaaacggag agaacctgat tatgcgccgg
     3241 atattatcag tcttactcga aaatgaatca ggcgcgttat cccgcgtgat tggccttttt
     3301 tcccagcgtg gctacaacat tgaaagcctg accgttgcgt caaccgatga tccgacatta
     3361 tcgcgtatga ccatccagac cgtgggcgat gaaaaagtac ttgagcagat cgaaaagcaa
     3421 ttacacaagc tggtcgatgt cttgcgcgtg agtgagttgg ggcagggcgc gcatgttgag
     3481 cgggaaatca tgctggtgaa aattcaggcc agcggttacg ggcgtgacga agtgaaacgt
     3541 aatacggaaa tattccgtgg gcaaattatc gatgtcacac cctcgcttta taccgttcaa
     3601 ttagcaggca ccagcgataa gcttgatgca tttttagcat cgattcgcga ggtggcgaaa
     3661 attgtggaag ttgctcgttc tggtgtggtc ggactttcgc gcggcgataa aataatgcgt
     3721 tgagaatgat ctgaatgcgc aatttgcagc ccaacatgtc acgttgggct ttttttgcga
     3781 aatcagtggg aacctggaat aaaagcagtt gccgcagtta attttatgcg cttagatgtt
     3841 aatgaattta acccatacca gtacaatggc tatggttttt acattttacg caaggggcaa
     3901 ttgtgaaact ggatgaaatc gctcggctgg cgggagtgtc gcggaccact gcaagctatg
     3961 ttattaacgg caaagcgaag caataccgtg tgagcgacaa aaccgttgaa aaagtcatgg
     4021 ctgtggtgcg tgagcacaat taccacccga acgccgtggc agctgggctt cgtgctggac
     4081 gcacacgttc tattggtctt gtgatccccg atctggagaa caccagctat acccgcatcg
     4141 ctaactatct tgaacgccag gcgcggcaac ggggttatca actgctaatt gcctgctcag
     4201 aagatcagcc agacaacgaa atgcgctgta ttgagcacct tttacagcgt caggtcgatg
     4261 ccattattgt ttcgacgtcg ttgcctcctg agcatccttt ttatcaacgc tgggctaacg
     4321 acccgttccc gattgtcgcg ctggaccgcg ccctcgatcg tgaacacttc accagcgtgg
     4381 ttggtgccga tcaggatgat gccgaaatgc tggcggaaga gttacgtaag tttcccgccg
     4441 agacggtgct ttatcttggc gcgctgccgg agctttctgt cagcttcctg cgtgaacaag
     4501 gtttccgtac tgcctggaaa gatgatccgc gcgaagtgca tttcctgtat gccaacagct
     4561 atgagcggga ggtggctgcc cagttattcg aaaaatggct ggaaacgcat ccgatgccgc
     4621 aggcgctgtt cacaacgtcg tttgcgttgt tgcaaggggt gatggatgtc acgctacgtc
     4681 gcgatggtaa actgccttct gacctggcaa ttgccacctt tggcgataac gaactgctcg
     4741 acttcttaca gtgcccggtg ctggcagtgg ctcaacgtca ccgcgatgtc gcagagcgtg
     4801 tgctggagat tgtcctggca agcctggacg aaccgcgtaa gccaaaacct ggtttaacgc
     4861 gcattaaacg taatctctat cgccgcggtg tgctcagccg tagctaagcc gcgaacaaaa
     4921 atacgcgcca ggtgaatttc cctctgg
//
LOCUS       DAZPAC010000271         4862 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZPAC010000271 DAZPAC010000000
VERSION     DAZPAC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.273"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            40..1020
                     /locus_tag="ACHUPM_003989"
     CDS             40..1020
                     /locus_tag="ACHUPM_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR0871634.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            1013..1429
                     /locus_tag="ACHUPM_003990"
     CDS             1013..1429
                     /locus_tag="ACHUPM_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR0871635.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(1431..2705)
                     /locus_tag="ACHUPM_003991"
     CDS             complement(1431..2705)
                     /locus_tag="ACHUPM_003991"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFR0871636.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPDFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(2705..3142)
                     /gene="umuD"
                     /locus_tag="ACHUPM_003992"
     CDS             complement(2705..3142)
                     /gene="umuD"
                     /locus_tag="ACHUPM_003992"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR0871637.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(3139..3387)
                     /locus_tag="ACHUPM_003993"
     CDS             complement(3139..3387)
                     /locus_tag="ACHUPM_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0871638.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            3781..4707
                     /locus_tag="ACHUPM_003994"
     CDS             3781..4707
                     /locus_tag="ACHUPM_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR0871639.1"
                     /translation="MPNWCSNRMYFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLF
                     LAGSAGLLQTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLML
                     HELWLQSGTGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLP
                     EKTMPFDLLTVLPTRLDIEVNGFNGGVLNCVPSAYHWYTERYGVKWPCGYDLNISSQG
                     ENFIQVDFDTPWCQPESDVVAELSRLFGCTLEHWYAEQGCDFCGWQLYERGELVDVLW
                     GELEWSSPTDDDELPEVTGPAWIVDNVAHYGG"
     gene            4704..>4862
                     /locus_tag="ACHUPM_003995"
     CDS             4704..>4862
                     /locus_tag="ACHUPM_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871640.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RWARDGRFY"
BASE COUNT         1122 a         1177 c         1289 g         1274 t
ORIGIN      
        1 ttgttttttc tatttgttta tgagcaacgg agactggtta tgaaaatctt tattgatgat
       61 ggttctacca acattaaact tgcctggttg gaggatggtg acgtgaaaac gttaatcagc
      121 cctaacagct ttaaaccgga atggtctttt agtttactgg atgatgctgc tccggctaat
      181 tacgagatcg acggcgagaa gttttcattt gatccattaa gtgctgatgc tgtcgtaacg
      241 actgaaacac gctatcagta cagtgatgtg aatgttgtgg ccattcagca tgcgctacag
      301 cagaccggcc tgaaagcgca gcctgttgat gtcatcgtca cattgccgat ctctgaatac
      361 cttgacgcca acaatcagaa aaataagcaa aacattgaaa gaaagaaaaa aaatgtgatg
      421 cgtgaagtac gggtgcaggg gagtgatgca tttgttattc gcagtgtttc cgttcttcct
      481 gaaagtattc ccgctggttt cagtgttctt gcggggcttg aggatgatga atcccttttg
      541 atcgtagatc tcggtggtac aactcttgat gtgtcgcatg ttcgtagcaa aatgacaggg
      601 attacaaaaa cctggtgtga tccgaatatc ggcgtgtccc tgattacatc cggggttaag
      661 gaacagatgg ctgtacatgc taatacaaga gtgagctcat tccaggctga caatatcatt
      721 gtgcaccgta atgaacctga ctacctttcc cgtcgcattt ataatgctga acagcgtgag
      781 tctatcatta atgttatcaa tgaacggcag aagttgctta ttaaacgagt taatgatgtc
      841 atcagccgtt tcaccgacta tacacatgta atgtgtgttg gtggtggggc tgaaattgtg
      901 gccgaggctg tgaagaacct gactaaggtt cctgatgagc gcttttatct gagctcatcc
      961 ccgcagtttg atttagtcat gggaatgata aaaatgaaag gtggtgtgac taatgagtga
     1021 tgaaaataaa tccagacgct gctcatttga acttttcccg gatgaacgga caggagataa
     1081 gatagcagat gagctcattg caaatgaaaa actgaaggag cggggacgtt tcatgcgcgc
     1141 gatgttagtt actggtgcgg cgtttgcagc tatagataaa cgtcttcctt tgctgatatc
     1201 ggagttactg acagaaaata cgactctgga tgatattaat aaggtgattt ccagcgtaat
     1261 accaggtgct ttttcggttg aaaagaaatt gcttgaatta ctggaaaaac aatctggtct
     1321 ccatacgtct gtggattgca gtactcctct tacagagcag agtctgtcga gaaatgacgg
     1381 tgaagaccag accagaagaa acgctgaaaa tatgttcgga gatgactgaa ctaacccagc
     1441 ctggccaggg gaatatctcg ccatttagtt gtataacact gactcaacat ttcccgtttc
     1501 atttgccatt caggggcggt tcctcgcccc gcaaaccaca ctttcccttt tcccgactgg
     1561 ttcagttcat cgagtgtttt cattaacttt tcactgtttt tacggggctg gatttcatca
     1621 aataatcccg gctgcgctat acccgatggt gtgaaatcag ccagcatgac tcctgccttc
     1681 atatagcggt acccttcacg ccagacatgg tttaaggctc tgcatgcggc ggcaataatg
     1741 tcccggctgt cctgtgtggg caatggaagc ttttccacag cggcattgct gtaacagggt
     1801 tcttttactg caaaggggga tgtccgtaca aatgtcgtca cctgccggca atactgacgc
     1861 tccccacgta gtttctctgc ggcccgctct gcatactgaa caacagcctg gtgcatggca
     1921 tctttgtctg tgattcgttc accaaaactg cgactacaga caatctgctg ttttgccggt
     1981 ggtgcttctt ccagggatat gcaggactcg ccgttgagtt cgcgtaccgt acgctcaaga
     2041 atgacgctga agtttttccg gatgaatgcc gtgttagcct gcgccagctg cagtgctgtg
     2101 ttaataccca gcgcattcag cttttccgtc agtctgcgtc ctactcccca gacctcacca
     2161 actggctgca gccccagtag cttcaaggtc cgattacggt tttctgccgt cagcgcgacc
     2221 actccggaaa actgtggcca ttgctttgtt gcccactgtg cacttttagc cagcgttttt
     2281 gtaggcgcaa tgcccacccc catggtgagt cctgtccagc tctttacctg ttccctgagc
     2341 tgatgaccaa aaaaatccgg agagatgcaa tgatttatcc cccgcaaatc aataaacatt
     2401 tcatcaattg agtagggctc aactgcggga gaaagcgact ccagaacagc catgacccgt
     2461 tggctcatgc tgtggtacag cgcataattg ctggaaaata catgtatttt cttctccagg
     2521 cgcatctgtc tcacctgaaa ccagggctgc cccattctga tgccaagggc ttttgcctcc
     2581 gggctgcgcg cgatcacaca gccatcgtta ttgctgagta cgatgaccgg ttcgttgcga
     2641 aggtccgggc ggaaaacttt ttcacatgag gcgtagaaac tgttgatatc agccagtgca
     2701 aacattagcg taactcccgc gtcctgtgta tcacgtgagt gacaacacca aaaatacaga
     2761 tgttttccgg atacagtgtg cggaactccg ggctgtctga aaccggctcc agtgccgggc
     2821 gtgggcgcaa cagcagtcgt ttgacggtga actcaccgtc gatctcagcg ataacgatgt
     2881 ccccgtgttg tggtttttcg gccctgtcca ctaccagcag atcaccattc tgcacgccag
     2941 cctggttcat cgattcaccg ctggcgcgca gaaagaaggt ggctgcaggt ctgctgatgc
     3001 aatagctgtt cagatccagt tcctgctcag cataatcagt ggcaggcgaa ggaaaaccgg
     3061 cctggcaacg atcggcaaac aacggtcgca cgtaactgtc atcgcctgaa gggtcagccg
     3121 gacggtgata aacggtactc atgctgcagg cagccagctg tcgtcctgcc agatttcttc
     3181 cagcagagac aggatacgtt ctttatcctt gtcgttgcgg gctcctgtga cagatacaga
     3241 cggggcgctg ccctggtcta tacgcagcca gatttccggg tagtgtggtt tcagacgttg
     3301 ttcgatttca ttctgtagcg cctgaagtgt gcctgatttg aggttttttg tgctctggcg
     3361 gtcgaaaaga atttcaatgc ggatcatgtt ctcatctccc ttttctattg ctgtatgcat
     3421 atacagtatt ctaataactg aatccacaca cagtcaaatg tcattattgt gactaaaaaa
     3481 cgctcaactc tctgagagtg tggctgtatg cctgctgtgg actgctgtgt ccagcttatc
     3541 cacagcattt tgcgcacggt tctgtgaata aaatacctgg ttacccaggc cgtgccggca
     3601 cgttaaccgg gctgcatccg atgcaagtgt gtcgctgtcg ccggcctcct cacccggtca
     3661 cgtttcgtcg ttcctcctcc acgcgctgcg gcttcggggc cgcacctgca ttcgtatgcg
     3721 gtcgcccggt tacaggtgcg gcacggcctg atggaggccg catgtgagag gagaattccc
     3781 atgccaaact ggtgctcgaa tcgtatgtat ttttctggtg aaccggcaca gatcgctgag
     3841 attaaacgac tggccagcgg cgcagtcaca ccgttttatc gccgcgccac aaatgaaggt
     3901 attcagctgt ttctggccgg aagtgccgga cttctgcaga ccactgaaga tgtgcagttt
     3961 gaaccgtgcc ccggactgac ggctgccgga cgtggtgttg tatcgccgga gaatatcgcg
     4021 ttcacacgct ggctgacaca cctgcagaac ggtgtgctac tggatgaaca aaactgcctg
     4081 atgctgcatg aactctggct gcagagcggt actggacagc gtcgctggga aggattaccg
     4141 gacgatgtca gggataccat caccgcactt ttcaccgcaa aaagaggtga ctggtgtggc
     4201 ttctggagta acgaggatgt atcggtgtgg tggaaccgtc tgtgtgacaa cgtactgccg
     4261 gaaaaaacca tgccgtttga cctgctgacg gttttgccga cccgcctgga tattgaagtg
     4321 aatggcttta acggcggtgt tctgaactgt gttccttctg catatcactg gtatacagaa
     4381 cggtatggcg tgaagtggcc ttgtgggtat gacctgaata tcagcagtca gggggaaaac
     4441 ttcattcagg tagatttcga cacgccgtgg tgtcagccgg aaagcgacgt tgttgcagaa
     4501 ttaagccgcc ttttcggctg cacgctggag cactggtacg ccgaacaggg ctgtgatttc
     4561 tgtggctggc agttgtatga gcgcggagag cttgttgatg tgctgtgggg ggaactggaa
     4621 tggtcttccc cgacagatga cgatgagctg ccggaagtca ccgggcctgc gtggatagtc
     4681 gacaatgtgg cgcattatgg cggatgaagt atgacggaga cgggcgggca accgcccgtt
     4741 tcttttccgg taaaggatgt cgccgtgctc ctctttttac tgcgccggct gacgcggcgc
     4801 ggcataaacg ctgcctgtgg tcagtgtccc gcgtccggtg ggcacgggac gggcgctttt
     4861 ac
//
LOCUS       DAZPAC010000272         4852 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZPAC010000272 DAZPAC010000000
VERSION     DAZPAC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.274"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..2689)
                     /gene="nikB"
                     /locus_tag="ACHUPM_003996"
     CDS             complement(<1..2689)
                     /gene="nikB"
                     /locus_tag="ACHUPM_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HFR0871641.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFLQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRNERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPP"
     gene            complement(2700..3032)
                     /gene="mobA"
                     /locus_tag="ACHUPM_003997"
     CDS             complement(2700..3032)
                     /gene="mobA"
                     /locus_tag="ACHUPM_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFR0871642.1"
                     /translation="MSDKRQRSGSERRQKTILRAVRFSPDEDETICKNAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            3265..3600
                     /locus_tag="ACHUPM_003998"
     CDS             3265..3600
                     /locus_tag="ACHUPM_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFR0871643.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     ILVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(3687..4535)
                     /locus_tag="ACHUPM_003999"
     CDS             complement(3687..4535)
                     /locus_tag="ACHUPM_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFR0871644.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRYLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
BASE COUNT         1165 a         1310 c         1153 g         1224 t
ORIGIN      
        1 aaggcggttc ccagcgttta tcctgctccg gcggaggcag tgatgcgtca tcgttgctgt
       61 tcgccgcgat ataatcgcgg tagtagcgtt cacttccttc acggtgatga tccacgtccg
      121 gtcgcgtaat cctgtagtct tcgtgaccgg tacggcctgt cacattatgc caggcaacca
      181 tttcagcaaa gacctggtta aattgtttgt cgtttccttc tggttcaaaa cccatgcgtg
      241 gatcacgccc gaataatacc ggtgcaatca aatccagttt atctgccagc gcattgcggt
      301 catggtgatt acggaaaaca accctgtcac cataatccgt acagacaaac tctcccgtcc
      361 gacgatcacg gaaacgaaca ctcccgttct tctgcaaacg ggcctcgaga tcaccggtat
      421 taccgtatac cggcgttccg cccggttcac aaagcacgac acagcggtct gttgtcgttg
      481 tgcgtgactt atctttacgg cgatcgcgat aatcccagcc tctcagctgc gatacagcag
      541 ccctgtctcc ctgagcagcc tgaatttcga cccactgacg gtaagagggt ggataccact
      601 tcccgtcagc aataagtttc tgccgctcat tcctgatgtc ttctttcagc ctgatcaatg
      661 cctgcattcg ctgaacttct gcaatgtgat aatgcagctt acgtaatgcc ggatcatcgt
      721 actgcgcacg aatgtgtgac ttccgcagac ggcatgcctg atgaatttcc cgacaacgtt
      781 ccccataacg cagatctggt ttacgccatt gttcacgcca tgccagatag cgggcacgca
      841 ggtcagcacg ggcctcagct ctggcttcac gtcgctgccg gcgcagcatt gggtcacgtt
      901 tactgctgac cccgtacctg tcactgacag ccagctccgg gttatagcga ctttcaggtt
      961 gcaccctgtc aaacaaatct gctggggcac tcacaaaagg tccggcctgt ggctctgcac
     1021 gccccagagt cagatcagga tgaatgctgc tggccttcac cggcgtttgc tcatgattaa
     1081 aggcatcaac gacaacaagc ccgtgatgtt gtttctgtag catcagtccc tgttgtgcaa
     1141 acaactcatg acaacgctgc caggtaaact caccctgtgt gatcatttct tcaatctgag
     1201 ggctacaatg gctgatgctg tatccccaca gactttcttt gtcagaaaac agttcgcgtt
     1261 tgatagcccc ctgcggcccc gtacctgtac gcatacggtg ccattcttta tcggtcgcag
     1321 gacagctctc catacgacga ccacggaagc gttctgttgg cgtaacctgg aggaaaatat
     1381 ctgttggtac aggctgccag ccaccatcga gttgattcac attgtcgagc gtcagcagtg
     1441 accatacgct gtcagcacga acgggagtct ggttatgatc ataaccgtca cagataacca
     1501 ggtgaccatg ctgaacacgc atccataatc ccgcttcagc cagtgtgcgg tgaatagcct
     1561 ggcagttttc cagccgtcct tcccgtgcca tttccggcag acgttctcca agatgacgac
     1621 aggcatactg ctgcagactt tctgtttcag cgattttttc cgggcgaaca tcagctgcaa
     1681 tgaagtccgg attatagcgt cctggtgctt ccacctggct gaaaatgtct tttggtactg
     1741 gcgtgtagtc acccattttt ttcatgagtt tttctgcaca ccaggaggga ccaaacgagt
     1801 caagctgtac accttccctg ttgcgatccc agccatccat caccagaaat tttccgtcca
     1861 tctgagacag gtacagacca tcttccgcaa gacgacgatg cagggataac cagtcattta
     1921 caggttcact gcgtaaacca gcgaccgctg tctgcgcgac atactcgcga aaagtttgtt
     1981 tttttccgcg tgtccaagcg ttctggcgat cacgttcaac ggcagttttg cgaacgatac
     2041 gatttcccgg cgcatggacc cagcaaccgt tatccggagc aaaaccatgc ttcagttcaa
     2101 gctcacggca ggcacgactg agtttttcct gactccatga taatcggttc agataacccg
     2161 tttcagggtg tacccggtta acagccacat ggacgtgcag attatctgta tcagtatgca
     2221 cggcagagac atactgatgg tcggcaaggc cgagtgattt cagcgtatga cgtacactgt
     2281 cataaatctg ttccggacgc gggctttcat gtgactgcca ggaaaggatg tagtgaaaaa
     2341 caggatcagt gtcatctttt gcatagtgtg cctgccgggc aatgtactcc atatctgcag
     2401 cggcagtttc aagagaagta cagttatgaa agcaggtgac accataaaag ttgacccatt
     2461 cgcagccgtc cttcatgaca tcaaccagcg ccacgaatga ctcatttcga agacgtgtcg
     2521 cataatcaac aaggcgactg aaacggcttc ggtgcggttg ttcagcctgc gaagaagaag
     2581 ataaatccag ttcctcatcg gtcatgtcat cccggacaga gacgtaggat accaggtctt
     2641 cgaacgaaga cttgccgtcc cttctctttt tcggaatgac tgcattcacc ggagaatcct
     2701 caaccttcca ttaactgttt acgcaatgta tttgtaagtt cggttatggc aacaagcact
     2761 tctgcgtatt ccttgtcacc ggttcttttt ccctgaacaa acagatgctt ctgcatcctt
     2821 cccagtctca ttaactcctt cagaaaagca tcatctgtcc gggagttaat tcgcttattc
     2881 agtgctgcat ttcggatgta agcagataca gtaagtccgg aatcttcagc attcttacag
     2941 atagtttcat cctcatccgg tgagaaccgg accgctctca ggatcgtctt ttgccgtctt
     3001 tcactaccac ttctttgacg tttatcactc acggtcagct ccttactggg gtgtcggggc
     3061 gaagccctga ccaggaggca attgtataat cgcgcgtgcg cggttataca attgcacatc
     3121 ctgtcccgtt tttcaggcca ttatagcctg ttatttttgt gcatcatagt cttatgcaga
     3181 cggcagaggc agggcgcagc cctgccagga ggcatttttt gatgataaaa tcagacttca
     3241 acatgaaacg gagagaaagt gccaatggct tctaaaaaat tttactcaga tgatgacatc
     3301 cagctggcaa aagctgcttt atcggaattg ccggacctca ccgctcaacg gaaaacattg
     3361 cgtgattttc ttgatgccat tcgtgatgac atcattatcc tggtaaggac aaagggttat
     3421 acactggcag atgttcgtga cacgctcaaa aatgcagggt atgaagtagg agagaaggca
     3481 cttcgtgaca ttattcggga agcagaatcg aaaaaaccat cccgccgttc ttcctctaaa
     3541 acagcaccta aaaagacaga ttcaggcagg aaagatggca tcgatatgaa caacaattga
     3601 tgtgacaatg tcacatcgtg ttctgagatt atgatgatta cagaaaaaaa aagccccggc
     3661 ataaatacca gggcttttct gtttaatcag actcgtggcg gcaatgcacc tggataatgc
     3721 cgtgcaataa tatcattcag tttttcaaca aggtcagaac gcttaaagac aatgtgtacg
     3781 gtccctttct ggtagccacg caccgtaaaa tattcccctt ctaacagctt accgacattg
     3841 tcgccgttcc cgagaaattc accatactgc gccccgatgc tgttcctgaa gtcaggaacg
     3901 ttgcgcccct ccagcagata aaacggacgt accaggtcat ccagtgcctc ccgtccgctg
     3961 cagtccagcg tcacacgccc attactccag cggtacagca gattttcaat aatgattttc
     4021 ttacccaggc ggcagggatt attggttctg taatcccatg acaatttccg gtagacatca
     4081 atcagtccct gttcaaatgt ctgcatctta ctggcattca gatagcgaaa agttgccagt
     4141 acattgtcca gtgtgatttc aggacaggtg tcgctcatca gctggctgtt ccactcatca
     4201 cgctggcatg aactcatgaa ggtgtacatg ccagtatccg tcatcaggcg atcccagatt
     4261 ttccggtcgg caagacgagt gatattcttt tttgcttctt ccggtttatc catgcatcga
     4321 acggagtcag tcacgcagtc ttcaaacccg tagagaaaaa tgtcaccgga agcatcgagc
     4381 agaatcctgc gcgctgtcgc caggcattcc atcgcctgca tgaacagtgc aataccttcg
     4441 ttacgctgcg cgatgatgcg gtcgatagcc acagacggaa taacgtctgt cgtgccggca
     4501 gtattcaggt cagcagtggg taaagtctga ttcatcacat tgtctcctca tcgtcacgac
     4561 tgccgggtca gccaggtcat ttcccggagg agcgaagctc cttctgccgt ccgggtcttc
     4621 ggggatttcc ccgcagtgga cctttccgac gggggacgct caccacgtca cggggcagtt
     4681 gtccgggggc agacgcaaaa gtttttgacg cctgcggcaa aaacttttgt ggctggtaca
     4741 tctctcccct ggacgactga aacgggatgt ggtcgtgtat gtacccccgg aaagtccgtc
     4801 tgcggagaac aggccgcagt gcggcggcag acaggcagag acagctgcgc ca
//
LOCUS       DAZPAC010000273         4784 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZPAC010000273 DAZPAC010000000
VERSION     DAZPAC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.275"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHUPM_004000"
     tRNA            6..81
                     /locus_tag="ACHUPM_004000"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHUPM_004001"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHUPM_004001"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHUPM_004002"
     CDS             complement(299..1057)
                     /locus_tag="ACHUPM_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR0871645.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHUPM_004003"
     CDS             complement(1065..2168)
                     /locus_tag="ACHUPM_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR0871646.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="ACHUPM_004004"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="ACHUPM_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4452)
                     /locus_tag="ACHUPM_004005"
     CDS             complement(3427..4452)
                     /locus_tag="ACHUPM_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0871647.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1311 a         1236 c         1143 g         1094 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccttcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggcg acgttgagtt cggtatctgt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 atcagtgacg ccagtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cctcccctga ctgtaaagcg gtgaagcgtt cttttgcagt
     4201 gagcggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aacatctaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctgt acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccatc ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aggtgtgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc gatataacat gcta
//
LOCUS       DAZPAC010000274         4781 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZPAC010000274 DAZPAC010000000
VERSION     DAZPAC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.276"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHUPM_004006"
     CDS             complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHUPM_004006"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR0871648.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHUPM_004007"
     CDS             complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHUPM_004007"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR0871649.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         1048 a         1345 c         1235 g         1153 t
ORIGIN      
        1 agtgtgagct aaatcctgct tatgtcatca aaaatatcga ttccccaaaa gatttaatta
       61 atgggcaaaa aaaacggtcc tcatcagaga accgtattac ttattcgatc aaattagcgg
      121 atgaaaaata tccgccatga cacgctatta tttgatctgc gcgtgcattt cctgcaccga
      181 aattactttt tcggtcgcgt cggcattcag cgccatcgcg gtagcgaaac caccgttcag
      241 ggtggtgtca taatgcactt tatattgcag cgcactgcga cggatcacgc gggagtcttc
      301 aatcgcgcga cggcctgaag tggtgttgat gatgtaggta tattcgccat tcttgatgcg
      361 gtcctgaatg tgcggacggc cttcatgcac cttgttcacc agacgcggat taatgcccgc
      421 ttcgcccagc acaatcgccg tgccgtgggt cgcatccagc tcgaagccct gtttcagcag
      481 ttttgccgcc aggtccacca cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg
      541 accgtgtttc ttcatggtgg agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa
      601 ggtgcggccc acgcccatca cttccccggt ggagcgcatt tctggcccta acagcgggtc
      661 aacacccggg aatttattga acggcagcac cacttctttc accgagtagt acggcgggat
      721 aacttctttg gttacgccct gctcagccag cgatttgcca gccatcacac gcgccgccac
      781 ttttgccagc ggtacgccgg tggctttgga gacgaacgga acggtacgcg ccgcacgcgg
      841 gttaacttca atcaggtaga cttcgttgtt tttcaccgca aactgcacgt tcatcagacc
      901 gcgcacctgc aattcgaagg ccagtttctg cacctgctgg cgcatcacat cctgaatttc
      961 ctgacttaag gtgtaggctg gcagagaaca tgcggagtca ccggagtgca cgcccgcctg
     1021 ctcgatgtgc tccatgatgc cgccaatcag caccatttcg ccgtcgcaga tggcatccac
     1081 gtcaacttct accgcgtcat caaggaagtg atccagcaac actggcgcat cgttagacac
     1141 gctgaccgcc gtctggaagt agcgacgcag gtcagcttcg tcatagacga tttccatcgc
     1201 ccgaccgccg agaacgtaag acggacgtac caccagtggg tagccaatct ctttcgcctt
     1261 ctctaccgcc atttcaatag cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg
     1321 ctcaaccgca tgctggaagc gttcacggtc ttctgcacgg tcgatagcat ccgggctggt
     1381 gccgataacc ggtacgccag cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc
     1441 gccgtactga acgataacgc ctttcggctt ctcaatacgc acgatttcca gcacatcttc
     1501 cagagttacc ggctcgaagt agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc
     1561 cgggttacag ttaaccataa tggtttcgta accgtcttcg cgcagcgcca gcgaggcgtg
     1621 tacacagcag tagtcgaatt cgataccctg accgatacgg ttcgggccgc cgccgagaac
     1681 catgattttt tcacggtcgg tagacggatt cgcttcgcac tcttcttcat aagtggagta
     1741 catgtaagcg gtgtcggtgg cgaactctgc cgcacaggta tccacgcgct tataaaccgg
     1801 gtgcaggtca tactggtcgc gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt
     1861 tgccagacgc gcatcggcaa agcctttgcg tttcagctgg cgcaggaatt cagcgttcag
     1921 gccagtgatg cccacttccg ctactttctc ttccagacgc accagttctt caatctgtac
     1981 caggaaccag cggtcaatat tggtcaggtt gaacacgccg tccacagaca ggcccgcacg
     2041 gaaggcatcg gcgatgtacc agatacgctc tgcgccagca tctttcagtt cgcgacggat
     2101 tttggttaac gcttccgggt catcgaggct cactttcggg tcgaatccag tcgcaccgac
     2161 ttccaggccg cgcagcgctt tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac
     2221 ttcgccaacc gatttcatct gagtggtcag acggtcgtta gcaccagcga atttttcgaa
     2281 gttgaagcga ggaattttag taaccacata gtcgatggac ggctcgaagg aggccggagt
     2341 acgtccgcca gtgatgtcgt tcatcagttc gtcgagggtg taacccaccg ccagtttcgc
     2401 cgccacttta gcaatcggga aaccggtcgc tttcgacgcc agcgccgaag aacgggacac
     2461 gcgtgggttc atttcgataa caatcaggcg accgtttttc gggttcaccg caaactggac
     2521 attggaacca ccggtttcaa cgccgatttc acgcagcacc gccatcgagg cgttacgcat
     2581 gatttgatat tctttgtcgg tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt
     2641 atggatgccc atcgcatcaa agttttcgat agagcagacg atgatgcagt tgtcgttttt
     2701 atcacgcacc acttccatct cgtactcttt ccagccgatc agcgactcat caatcagcag
     2761 ctctttggtt ggggagagat ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt
     2821 ataagcgata ccgccgccgc taccgcccat ggtaaaggat gggcgaataa tgcacgggaa
     2881 gcccacgtca gcggcaaccg ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc
     2941 ggtttccaga ccaattttct tcatcgctac gtcgaaacga cggcggtctt ctgctttatc
     3001 aatcgcatcg gcggtcgcac caatcatagt cacgccgaac tcttccaaca cgccctgacg
     3061 ctccagctcc agcgcgcagt tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc
     3121 cgggcgctct ttttcaataa tcttgcgtac tacttcccag tgaatcggct cgatgtaggt
     3181 cgcatcggcc atttccgggt cggtcatgat ggtcgccggg ttggagttca ccagaatgac
     3241 gcggtaaccc tcttcgcgca gggctttaca tgcttgcgcg ccagagtagt caaactcaca
     3301 cgcctgaccg ataacaatcg ggcccgcacc cagaatcagg atacttttta tatctgtacg
     3361 ttttggcatg gctcttttac tcctgattac ttagcggttt tacggtactg ctcaattaac
     3421 tcgataaagt ggtcgaacaa cggcgctgcg tcgtgtggcc cagggctggc ttccgggtga
     3481 ccctggaagc tgaacgccgg tttatcagtg cgatgaatgc cctgtaacgt accgtcgaac
     3541 agggatttat gcgtgacacg caggtttgca ggtaatgttg cttcatccac cgcaaaaccg
     3601 tggttctggg cggtgatcat caccacgttt ttctccacat ctttaaccgg atggttgccg
     3661 ccgtggtgac caaatttcat tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga
     3721 tgaccgaggc agatgccgaa taccggaata tcggtttcga ggaatttctg gatggcggta
     3781 atggcgtaat cgcacggggc cgggtcgcca ggaccgttgg agaggaagat gccgtctgga
     3841 ttcattttca gcacatcttc cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct
     3901 ctatccacca gcatccgcag gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg
     3961 aacggcagct cgtcttcttt tttcgcttct ggcaggccgc cggtcaacgt ccagctccct
     4021 tgtgtccagc tataggcttc tgcggtggtc acttcttttg ccagatccat gccattcaga
     4081 cctgggaacg cgcgggcttt ttctaacgcc aacgccgcat ccgggttatc gcctgcgata
     4141 atgcagccat tctgtgcgcc tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata
     4201 tcggcaatcg ccacgatgtt atggcgcttc aggtaagaag agaggtcttc ggtattacgg
     4261 aagttgctgg caatcagcgg cagatcgcga atcaccagac cttgtgcatg tacctgagaa
     4321 gattcttcat cggcgtcatt agtgccgaca ttgccaatat ggggataagt aagagtaacg
     4381 atttgacgag aataggaagg atcagtgagg atttcttgat aaccggtcat tgaagtattg
     4441 aaaacgactt ccccaaccgc cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt
     4501 ccgtcttcca gaaccaatag cgctgactta atcaaaacac cctccagaga atattcactc
     4561 actttatttg catattaatt cacaatgatg acgtaaatca atgcaaatct gcttaccgac
     4621 gaatttctgg caaacggcgg cattctggag atatgggcgc taaaagtcaa cttaatggcg
     4681 gtatttttta tattattttg tggctcatgc atattttaca ggcattaagc gacaaaaaga
     4741 tcatataacc tgttctggaa aacgcttgcg cagcaagaag a
//
LOCUS       DAZPAC010000275         4767 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZPAC010000275 DAZPAC010000000
VERSION     DAZPAC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.277"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1107
                     /locus_tag="ACHUPM_004008"
     CDS             <1..1107
                     /locus_tag="ACHUPM_004008"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR0871650.1"
                     /translation="LITASCSTFAAPQQINDIVHRTITPLIEQQKIPGMAVAVIYQGK
                     PYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLSDPATKYWPE
                     LTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPGTQRLYANS
                     SIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWGYREGKAVHV
                     SPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYWQTGDMYQGL
                     GWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGATGGFGSYVA
                     FIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1196..1729
                     /gene="blc"
                     /locus_tag="ACHUPM_004009"
     CDS             1196..1729
                     /gene="blc"
                     /locus_tag="ACHUPM_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0871651.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1726..2043)
                     /gene="sugE"
                     /locus_tag="ACHUPM_004010"
     CDS             complement(1726..2043)
                     /gene="sugE"
                     /locus_tag="ACHUPM_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0871652.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2218..2364)
                     /gene="ecnB"
                     /locus_tag="ACHUPM_004011"
     CDS             complement(2218..2364)
                     /gene="ecnB"
                     /locus_tag="ACHUPM_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR0871653.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2475..2600)
                     /gene="ecnA"
                     /locus_tag="ACHUPM_004012"
     CDS             complement(2475..2600)
                     /gene="ecnA"
                     /locus_tag="ACHUPM_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR0871654.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2652..3218)
                     /gene="efp"
                     /locus_tag="ACHUPM_004013"
     CDS             complement(2652..3218)
                     /gene="efp"
                     /locus_tag="ACHUPM_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR0871655.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3260..4288
                     /gene="epmB"
                     /locus_tag="ACHUPM_004014"
     CDS             3260..4288
                     /gene="epmB"
                     /locus_tag="ACHUPM_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR0871656.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            4678..>4767
                     /gene="yjeJ"
                     /locus_tag="ACHUPM_004015"
     CDS             4678..>4767
                     /gene="yjeJ"
                     /locus_tag="ACHUPM_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR0871657.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPR"
BASE COUNT         1244 a         1187 c         1170 g         1166 t
ORIGIN      
        1 ttaattaccg cctcttgctc cacatttgct gcccctcaac aaatcaacga tattgtgcat
       61 cgcacaatta ccccgcttat agagcaacaa aagatccccg gtatggcggt ggcggtaatt
      121 tatcagggta aaccttatta ctttacctgg ggctatgcgg acatcgccaa aaagcagccc
      181 gtcacacagc aaacgttgtt tgagttaggt tcggtcagca aaacatttac gggcgtgctt
      241 ggtggcgacg ctattgctcg aggggaaatc aagttaagcg atcccgcaac aaaatactgg
      301 cctgaactta ctgctaaaca gtggaatggg atcacactat tacatcttgc gacctacacc
      361 gctggcggcc tgccattgca ggtgccggat gaagtgaaat cctcaagcga cttgctgcgc
      421 ttctatcaaa actggcagcc tgcatgggct ccaggaacac aacgtctgta tgccaactcc
      481 agtatcggtt tgttcggcgc actggctgtg aagccgtctg gtttgagttt tgagcaggcg
      541 atgcaaactc gtgtcttcca gccactcaaa ctcaaccata cgtggattaa tgtacctccc
      601 ccagaagaaa agaattacgc ctggggatat cgcgaaggta aggcagtgca tgtttcgcca
      661 ggggcgttag atgctgaagc ttatggtgtg aagtcgacca ttgaagatat ggcccgctgg
      721 gtgcaaagca atttaacccc ccttgatatc aatgagaaaa cgcttcaaca agggatacaa
      781 ctggcacaat ctcgctactg gcaaaccggc gatatgtatc agggcctggg ctgggaaatg
      841 ctggactggc cggtcaatcc tgacagcatc attaacggca gtgacaataa aattgcactg
      901 gcagcacgcc cggtaaaagc gattaagccc ccaactcctg cagtacgcgc atcatgggta
      961 cataaaacgg gggcgaccgg cggatttggt agctatgtcg cgtttattcc agaaaaagag
     1021 ctgggtatcg tgatgctggc taacaaaaac tatcccaatc cagcgagagt cgccgccgcc
     1081 tgtcagattc tcaacgctct acagtaaaat ttcatcgggt ccgaattttc ggaccttttc
     1141 tgcgcttttc cttgctgtca tctacactta gaaaaaaacc agtaaggaaa catttatgcg
     1201 cctgctccct ctcgttgccg cagcgacagc tgcatttctg gtcgttgcct gcagttctcc
     1261 tacgccgccg cgtggcgtga cagtagtgaa taatttcgac gctaaacgct atctcggtac
     1321 ctggtatgag attgcccgtt ttgatcaccg ttttgaacgt ggactggaaa aagtcaccgc
     1381 aacatacagt ctgcgtgatg acggcggcct gaatgtcatc aataaaggct ataaccctga
     1441 cagagaaatg tggcagcaga gtgaagggaa agcgtacttt accggcgcac caacacgcgc
     1501 tgcgctgaaa gtgtcattct ttggtccttt ctatggcggt tataacgtca ttgcacttga
     1561 tcgggaatac cgccatgcgc tggtttgcgg cccggaccgc gactacctgt ggatactctc
     1621 ccgcacgcca accatttctg acgaagtgaa acaggagatg ctggcagtcg cgacccggga
     1681 agggtttgat gtcagtaagt ttatttgggt acagcagccc ggtagttagt gagtgctgag
     1741 tttcagacca ataatcccca atacgattaa cgccagactc gccaggcgca tcgggttagc
     1801 ggactcaccg agcagcacaa tgccggtgat ggccgcgccg actgcgccaa tacccgtcca
     1861 cacggcataa gccgtcccta ccggtaacga tttcatcgcc caggcaagta gcgccatact
     1921 gactatcatc gccgtcacag taataacact tggcgtcaaa cgactaaagc cgtgggtata
     1981 tttcaggcca acggcccata ccacttccag cagaccagca ataactaaga taatccagga
     2041 catatcaggc tccggagaat tggggccatc cccggtgaaa agaggcgttt gcaggtcgtc
     2101 ctgcaaagct gatggttgaa cggatatttt gccaggtggc aggggaattt caactattac
     2161 ctttgtgact atcgcaaaaa aacaaacggc acgacataac tgtcgtaccg ttattgctta
     2221 ttgctgcgct ttcgttgctg caccagaaat cgcgttaccg ccatcagaaa tgtcttcacc
     2281 aacgccacgc gtggtgttgc aggcagttaa tactgttgaa agcaccagaa cagaaaagat
     2341 cgctgcaatt gtcttcttca ccataacgtc ttccttttag gtaatgttat ttatgtttgc
     2401 ctatagcaaa ttaataatag acaacatcgc caaaaatgat ggaaatctga tgatttttcg
     2461 gaagagaaga aaaattagct ggcagcgcgg gagatggagt tgccgagatg tttgatgtct
     2521 tcgccgaaac cgcgagcggt gttacagccc gtgagcagcg tgctggcaag caaaacaaga
     2581 acgataaggc gtttcatcat ctctgacctg aataagtgat ggtgcagcct gcaggccgca
     2641 ccacaaccgc attacttcac gcgagagacg tattcaccag agcgggtatc cactttgatg
     2701 acttcgccga tttgtacgaa cagcggaact ttaaccacag cgccagtaga cagggtagcc
     2761 ggtttgccgc cagtacctgc ggtatcacct ttcaggcccg gatcggtatc aacgatttcc
     2821 agttcaacga agttcggcgg agtaacggag atcggctgac cattccacag agttacgata
     2881 cactctgcct gatccagcag ccatttagcg ttgtcgccaa ttgcttttgc atcagcagac
     2941 agctgctcga aagtttcgtt gttcatgaag tgccagaact caccgtcgtt gtacaggtaa
     3001 gtcaggttca tatcgacaac atcagcgcct tcagcggaat cagtagattt gaaggttttt
     3061 tctacgcgag taccggtcag cagacgacgc agtttaacgc gagcaaatgc ctggccttta
     3121 cccggtttta cgaattcact cgcttcaacc gcgtaaggtt cgccgtctaa catgatttta
     3181 agaccagcac gaaaatcgtt gctatagtac gttgccataa ggccctctga aatttgttaa
     3241 ttggtagcta agccacaaaa tggcgcatat tgtaacccta aataccccat ccagagaaga
     3301 ttggttaacg caacttgccg atgttgtgac cgatcctggt gaacttctgc gtcttttgaa
     3361 tatagacgcg gacgaaaaac tgttggccgg acgcagcgcc aaaaagctgt ttgccctgcg
     3421 tgtgccccgc tcatttatcg atcgcatgga gaaaggcaat ccggacgatc ctattttgcg
     3481 tcaggtactt acctcgcaag atgagtttgt cgtcgcgtcc ggattctcca ccgacccact
     3541 ggaagaacag cacagcgtag tgcctggttt gttgcataaa taccacaacc gggcgctttt
     3601 gctggtcaaa ggcggctgcg cggtaaattg ccgctattgc ttccgtcgcc acttccccta
     3661 tgccgaaaat cagggcaaca agcgtaactg gcagactgca cttgagtatg ttgctgcgca
     3721 tccggaactg gacgagatga ttttctccgg cggcgatccg ctgatggcga aagatcacga
     3781 gctggactgg ttgctcacac aactggaagc catcccgcat atcaaacgtc tgcggattca
     3841 cagccgtctg ccgattgtga tcccggcacg tatcaccgag gcgctggttg aacgctttgc
     3901 ccgttctacg ctgcaaatct tgctggtgaa tcacatcaat catgccaatg aggtagatga
     3961 aacgttccgt caggcgatgg ctaaattgcg ccgtgtcggt gtcaccctgc ttaaccagag
     4021 cgttctgtta cgtggtgtga acgataacgc acaaacgttg gcaaacctga gtaatgcgtt
     4081 gttcgatgcc ggcgtgatgc cgtattacct gcatgtgcta gataaagtac agggcgcggc
     4141 gcattttatg gtgagtgatg acgaagcacg gcagattatg cgtgagttgc tgacattggt
     4201 gtcgggatat ctggtgccga aactggcgcg agaaattggc ggcgaaccca gcaaaacgcc
     4261 gctggatctc cagctacgcc agcagtaact gattaaacag ggaagttaaa cattagcctg
     4321 atgcgctacg cttatcaggc ctacattgtg cattgaaatt gaatggattt gcaatgaact
     4381 gtaggccgga taaggcgttt acgccgcatc cggcaagagc aacaaacgca agtacagcaa
     4441 cctgtcatta aactgggaaa tttaatcttt gcccgttgca taacctttaa ttattcatat
     4501 acattgctgt tgttactctt tatatagcca acaccaataa ttaacctttc ttttctgttt
     4561 atatagagtg agtacggcta tttatgttat tttaacaact attactacca agcccattta
     4621 tctggcttat aaattaaaat taattattta attgagaaat ggttagggag aacctacatg
     4681 gccataagca tcaagggtgt taataccggt gttattcgca agagtaataa tttcattgca
     4741 ctggcgctga aaattaaaga acctcgc
//
LOCUS       DAZPAC010000276         4741 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZPAC010000276 DAZPAC010000000
VERSION     DAZPAC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.278"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..737)
                     /locus_tag="ACHUPM_004016"
     CDS             complement(<1..737)
                     /locus_tag="ACHUPM_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR0871658.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHUPM_004017"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHUPM_004017"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR0871659.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="ACHUPM_004018"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="ACHUPM_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR0871660.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="ACHUPM_004019"
     CDS             4397..4678
                     /locus_tag="ACHUPM_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR0871661.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1147 a         1177 c         1183 g         1234 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg atttttattg atatcaacaa taccatttac ataaatctac attaattgat
     4741 a
//
LOCUS       DAZPAC010000277         4716 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZPAC010000277 DAZPAC010000000
VERSION     DAZPAC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.279"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            99..1073
                     /gene="hemB"
                     /locus_tag="ACHUPM_004020"
     CDS             99..1073
                     /gene="hemB"
                     /locus_tag="ACHUPM_004020"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR0871662.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1179..2030)
                     /gene="tauD"
                     /locus_tag="ACHUPM_004021"
     CDS             complement(1179..2030)
                     /gene="tauD"
                     /locus_tag="ACHUPM_004021"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR0871663.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2027..2854)
                     /gene="tauC"
                     /locus_tag="ACHUPM_004022"
     CDS             complement(2027..2854)
                     /gene="tauC"
                     /locus_tag="ACHUPM_004022"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR0871664.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2851..3618)
                     /gene="tauB"
                     /locus_tag="ACHUPM_004023"
     CDS             complement(2851..3618)
                     /gene="tauB"
                     /locus_tag="ACHUPM_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR0871665.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3631..4593)
                     /gene="tauA"
                     /locus_tag="ACHUPM_004024"
     CDS             complement(3631..4593)
                     /gene="tauA"
                     /locus_tag="ACHUPM_004024"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0871666.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1075 a         1379 c         1220 g         1042 t
ORIGIN      
        1 cattctcttc ttgtcaacat cacgacaact ttcgtaaaac actcctgccc cgcttcaggt
       61 atactgtgca ccttaattcc acaaacatca ggcagaccat gacagactta atccaacgcc
      121 ctcgtcgcct acgcaaatct cctgcgctgc gcgctatgtt tgaagagaca acacttagcc
      181 ttaacgacct ggtgttgccg atctttgttg aagaagaaat tgacgactac aaagccgttg
      241 aagccatgcc aggcgtgatg cgcattccag agaaacatct ggcacgcgaa attgaacgca
      301 tcgccaacgc cggtatccgt tccgtaatga cttttggcat ctctcaccat accgatgaaa
      361 ccggcagcga cgcctggcgg gaagatggac tggtggcgcg tatgtcgcgc atctgcaagc
      421 agactgtgcc agaaatgatc gtcatgtcag acacttgttt ctgtgaatac acttctcacg
      481 gtcactgcgg tgtgctgtgc gagcatggcg tcgacaacga cgcgactctg gaaaatttag
      541 gcaagcaagc cgtggttgca gctgctgcag gcgcagactt catcgcccct tccgccgcga
      601 tggacggcca ggtacaggcg attcgtcagg cgctggacgc tgcaggcttt aaagatacgg
      661 cgattatgtc gtattcgacc aagttcgcct cttcctttta tggcccgttc cgtgaagctg
      721 ccggaagcgc attaaaaggc gaccgcaaaa gctatcagat gaacccaatg aaccgtcgcg
      781 aggcgattcg tgaatcactg ctggatgaag cccagggcgc agactgcctg atggttaaac
      841 ctgctggagc gtacctcgac atcgtgcgtg agctgcgtga acgtactgaa ttgccgattg
      901 gcgcgtatca ggtgagcggt gagtacgcga tgattaagtt cgccgcgctg gcgggtgcta
      961 tagatgaaga gaaagtcgtg ctcgaaagct taggttcaat taagcgtgcg ggtgcggatc
     1021 tgattttcag ctactttgcg ctggatttgg ctgagaagaa gattctgcgt taattttttt
     1081 cctctcagaa agggggagtt aacctgttga aagtacggtg aacgccccct caccctaacc
     1141 ctctccccaa gggggcgagg gaaccgtccg cactcgtatc accccgcccg ataaaacggt
     1201 ttatccccaa ggatcgtcgc ccgatgcata atccgccgct gtggcaggta atcggcattg
     1261 gcatagtgct gagtcacgcg gttatcccaa atcgcaatat cattcggttg ccagcgccaa
     1321 cgcacctgaa actccggttt agtgatatgg gcgaataaaa agcctaacaa ggcttcgctc
     1381 tctttctcgc tgacatcgac aattcgcgta gtaaagcctt cattcacaaa cagcgcctgt
     1441 ttaccgctca ccggatgcgt tcgcaccacc ggatgtagca acggaggatt tttcgcgacc
     1501 gcctcgcgcc agcgttgatg ttcctcctcg gttttgcggt atttgtattc cgggaacgat
     1561 ttacggaaat catgctccgc acgcagcccg ctcagcagct ggcggaaggg aacagagagc
     1621 gcctcatagg ccgcaatacc gctggtccag agcgtatcac cgccggtcga aggtaactct
     1681 ttagctgcca gaatcgcccc tgcgggtggc gtttcaataa atgtcacatc ggtatgccag
     1741 ttgtcgttat ccggcggatt atcgttatgg gtatccagca cgatgatctc gtcaacccct
     1801 tcggcatgag ggtaaacagg gtgaatatgc agttcgccaa aacgctgggc cagcgcgcgt
     1861 tgctgctgcg gcgtaatagc ttgatcgcgc agaaatacca cctgatagcg cagcaccgca
     1921 tggtaaagct gttcaaactg attatcgctt aacgggcgcg tcaggtcggc acccgaaatt
     1981 tgtgcgccga tatacggccc cagcggggta atactcagac gttcactcat tgtacttctc
     2041 catgccaggg cgtcagacgg cgctgtaacg cgcgcagacc cagttctaaa agaaaggcga
     2101 taatcgcaat caccgcgatc cccgccagta ccacgtcagt cgcgagaaat tcacccgctg
     2161 actgaaccat aaatcctaaa ccgcgtgtcg cggcaatcag ctccgccgct accagcgtag
     2221 accagcccac acccagacca atgcgtaatc cggtgaggat ttccggcagc gcgccgggca
     2281 aaatgacaaa ccacagcacc tgcgcacggc tggcacccag cgactgggcg gcacgaatgc
     2341 gaacctgctg cgcgcttttc acccccgcca gcaccgacat cgccaccggt gcaaaaatcg
     2401 ctaaatagat caataagatc ttcgaggttt caccaatacc aaaccagatc accatcagcg
     2461 gcaaataagc cagcggcggc accggacgat aaagttcgat taccggatcc agaatgccgc
     2521 gcaccgtcgg gcttagcccc atggcgatcc cgaccggaat accgatcacc accgccgcga
     2581 gtagcgccag cacaatgcgc gtcaggctgg ctgccagatg ctgccacagc gtggcgtcca
     2641 taaatccctg cggtccggca atggtgagca gttttgccag tacttgttgc ggcggcggca
     2701 aaaacagcgg gctaatcagt tgcagcgccg ccaccgtcca ccatacagtg agtaaaaccg
     2761 ctaacgtgcc aatgcttaag gtcacctgac gcgagagcgg ccagcgccat ttcagccgcc
     2821 gcgaatgcag tttttcatta atgagcacac tcatgagaac gcctcccgtt gctcaaatac
     2881 gcggcttaaa acatattcgc gcatggcgat gaattgtgga tcggacttga tgctgcggct
     2941 cgactctccc gcaacaaagc ggcgagcaaa gttgagcggc agccgctcca gcacacggcc
     3001 agggccggat gaaagcagaa ccagttcagt cgccataaac accgcttctt ctatatcgtg
     3061 ggtaatcaac agcacctgct tgcccgtctc ctgccagagt ttcagcagca gagtttgcat
     3121 ctggtcgcgg gtgaaggcgt ccagcgcacc aaacggttcg tcgagtaata acagctgggg
     3181 attcgccgcc agcgcgcggg caatccccac ccgctgacgt tgaccaccgg aaagctgcca
     3241 gatatagcgt ttttctgcgc cttccagccc cacttttttc agcatctggt gcgcgatttc
     3301 cagtcgctgc attttctcta tacctgccaa ttgcaggccg aacgccacgt tgtcctgtac
     3361 attgcgccac ggtagtagcc cttcattctg aaaaaccacc ccgcgctctg ctcccggtcc
     3421 ctcaatacgc ttacccgcca gttgaatgct gccatgctga taaggcacaa aaccggcaat
     3481 cagattcagc agggtggttt taccgcagcc ggacggcccc agcaccacca gtagctcgcc
     3541 gctttccagc gtcaggttga tatcttccag tgccggtttg ccgccataat cggcatagag
     3601 atgagagatt tgcagcatct gcgcctcctt ttattgcacg aagcgcgagg taacgtactg
     3661 gctgtaatca ttcgctacag ccgggacctt gccctgctct ttcaaaaact gcgcggtgtc
     3721 gatgattgct ttgttcaccg gtccggtcag ttctgccgtt tgttgctgcg gcgtcagata
     3781 ggtattcccc ttcaccagcc ccggaacgtc accttccggc acgccgctta aacgcgccag
     3841 tttgctgatg ttttccggct gtttcagcca cgcgtctggg ttagcaatgt acggttgctg
     3901 agcatcgatg gcgcttttag cgaacgcttt cacgacctca ggatgtttct cggcaaaatc
     3961 tttgcgcacc acccagacgt ccagcgttgg cgcaccccac tgcccgacct gttcagaatc
     4021 ggtcaacacc ttgccgtctt tttccagggc gttaaccgcc ggtgcccaga cataagcacc
     4081 atcaatatca ccccgctgcc atgcggcgat aatcgcgggc ggctgcaggt tcacaatctc
     4141 cacttgccct ggtttaatcc cccagtgttt cagcgccgcc agcaggctgt agtgggtggt
     4201 ggagataaac ggtacggcaa tgcgtttgcc aatcaaatct tcgggtttgc tgatagtttt
     4261 ctttaccacc agcgcttcgg agttacccag ttttgacgcc agcaagaaga cttcaatcgg
     4321 cacctgttgg ctggctgcaa ccgctaatgg gctggaaccg aggttgccga tttgcacgtc
     4381 gccagaagcc agcgcccgca cgatgctggc tccgctgtca aacttacgcc agtccacgct
     4441 cgccccgctc tctttagcaa aggtgttgtc ggcctgagcc actttcgccg gttcggctga
     4501 ggtttgatac gccacggtga cgttcaccgc ctgtgcctga aaagcgatga atgccagtgc
     4561 ggcaagaagt gtgttacgcg atgaaattgc catgattgtc tgctcccctg tcttgttatg
     4621 ggagcagtat tcaggaataa aaacattcat taaaagaatt agtcgttatc gcacagatga
     4681 ttttattctt agcaaaaaaa cggcaaagga aagatt
//
LOCUS       DAZPAC010000278         4708 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZPAC010000278 DAZPAC010000000
VERSION     DAZPAC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.280"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..447
                     /locus_tag="ACHUPM_004025"
                     /pseudo
     CDS             1..447
                     /locus_tag="ACHUPM_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(489..941)
                     /locus_tag="ACHUPM_004026"
                     /pseudo
     CDS             complement(489..941)
                     /locus_tag="ACHUPM_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1005..1151
                     /locus_tag="ACHUPM_004027"
                     /pseudo
     CDS             1005..1151
                     /locus_tag="ACHUPM_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1151..2308)
                     /gene="intS"
                     /locus_tag="ACHUPM_004028"
     CDS             complement(1151..2308)
                     /gene="intS"
                     /locus_tag="ACHUPM_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR0871667.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2470..2544)
                     /locus_tag="ACHUPM_004029"
     tRNA            complement(2470..2544)
                     /locus_tag="ACHUPM_004029"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2509..2511),aa:Arg,seq:cct)
     gene            complement(2620..3552)
                     /locus_tag="ACHUPM_004030"
     CDS             complement(2620..3552)
                     /locus_tag="ACHUPM_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR0871668.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3846..4601
                     /gene="mlaA"
                     /locus_tag="ACHUPM_004031"
     CDS             3846..4601
                     /gene="mlaA"
                     /locus_tag="ACHUPM_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR0871669.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         1135 a         1128 c         1102 g         1343 t
ORIGIN      
        1 ctgtgctgaa tacatagaaa tttttgatga gtctattgct tttacatgtc tctctgcatt
       61 ttcttctgaa aaacaatttg gagtatttct gtttggatgt ttgaaaagca cagggctaaa
      121 gttaaaacga ttattcatac ggatttatct gcaccatggc gtcttaagga tatatcatca
      181 agattatatc tcagcgaaag tttactaaag atgaaattga aagaagaggg ggtatcattc
      241 agtaagatca tacttgatga gaggatgcaa atggctgaat atttactcag cactcgttgt
      301 tatcctatta gtaaagtagc taaggtctgt ggttatgcca gtgtctcata ctttacttat
      361 gtatttagac gttattttgg tgtttctcca agtcaatact ctcagaggag ttcagaaagt
      421 aaaattctta ctcaccaggg aatctgatca ttgttcttgc ccccttattt ccagacaggg
      481 ggtgtatctt aagttaacgt tacccgctga cgtcgatatt ctcgcggaga gcgataaccc
      541 agcgcactgt gcggatggtt ttcattgtaa tgttcgatcg ccactgcaag attatgcaat
      601 gccgttctta cattcggttt cggcatgaac gcgatgtagt cttccttcat cgttttcacg
      661 aacctttctg ccattccatt actctgagga ctgctgattg ccgttgtgca gggctccaga
      721 ttcaactctc tggcgaactg ccgtgtttca tgcgctctat acgctgaacc gttatctgtc
      781 agccactgga tggactgttc cggtacttta tcgccaaagc gtttttctat cgctcctaac
      841 atgacatcct gcactgtcgc tttatcgtaa cctcccgtgc ttgcggccca gtctatggct
      901 tcacgatcgc aacagtccag ggcgaacgta acacgcagtt ttgaatcgcc acgggtttaa
      961 cagacacctc agagtcattt aagatggctt aaagagaggt gcccatgagc ggtaagcgtt
     1021 atcccgaaga gtttaaaact gaagcagtca aacaggttgt tgatcgcggt tattctgttg
     1081 ccagcgttgc aacacgtctc gatatcacta cccacagcct ttatgcctgg ataaagaagt
     1141 acggtccgga ttactccact ttttcatcaa gccaatccgc ccaccactgc atcatttctc
     1201 tgcgcttatc gagatactga gcatggttgt aaataccgcg cacagatccg ccgttggcat
     1261 gtgccagctg cacttcaata gcgtcagcag gccattcgtg ctcgttcata atcgtgctga
     1321 attcatgcct gaatccgtga ccgctttcca gaccttcata gccgatttgt ttgatcacaa
     1381 gcaataccgc gttctcgcag attggcttct tcttatcgtt gcgcccggca aaaacaaact
     1441 ctgagactgg tttggtgatt gagcttagcg tagtgagaag ttcaaccacc tggtctgaca
     1501 tcgggaccac atgaattttg cgtcccttca tcacactggc gtcgatggtg ataatcctgt
     1561 tttcaaaatc gacgttcttc catagcatgg aacgaagctc ttttgttctt agggctgtgt
     1621 agcgtaaaac tttggtcgca atgagcgata cgatacttcc tgaaaatgtt gccagtgctt
     1681 tgttgaatgc aggcatctgg tctgctggaa ggaacgggaa gttcttcttg cggtatcctt
     1741 tcatggcgtc tgcaaggtca ggtgccgggt tatatttagc ccttccggtg acaatagcgt
     1801 aacgaaaaac ctcgccgcat cttctgcggg ctttgttggc tcgctccatt gcaccgcgat
     1861 cttcaaatct gcggattact tccagcagtt gcatcggctc aatatcctga atctcaagac
     1921 cgccgatgat gggtaaaatg tcgtcatcaa acattttggc aagttcagtt gcatagccta
     1981 ctgaccagac ttgcttcttg tgctcgtacc attccttgta aatcgcacta aatgaattgt
     2041 tgttagacga agcctttttc gcctttaccg gaacgatgcc aaccgagatg tctttcctcg
     2101 cagtccatgc cttatccctt gcttcctgca aagtcataag cggatatttt ccgacggtca
     2161 ggattttctc cttaccgtca atcttgtagc gaagctgcca tacctttttc ccggatacag
     2221 gaacataaag gtacaggcca ttaccatcga gaaggcggta tggtttttct ttcggctttg
     2281 ctgcttcaat ctgcttaacg gtgagcatgg gtaaaaatcc ggtgggtaaa attattttat
     2341 ccacttttta cccgtcatgg agtgcggctg tcaacgatct gacgcgaacc atgacgaacc
     2401 gtgaatatac ggaaggcttg atattcaggg gattttgcgg actggtacgg atgggagcga
     2461 actgataaat ggtgtcccct gcaggaatcg aacctgcaat tagcccttag gaggggctcg
     2521 ttatatccat ttaactaaga ggacaatgcg gcatgagtat acccgctaat ggagtgcggg
     2581 gtaagtacgc tgccgctcga ttgcttaaac cctcgccatt tatgccgggt ttttatcatt
     2641 tttcttaatg ttttccgcac gttctgcttt ttggcgtgct tctgctttac gcttgttgct
     2701 catgtcgtta cgaatctgtg catgactcat taacgcgaag ataaaggtgc cgccgcagat
     2761 gttccccgct aaagtaggta gtgcgaaggg ccagatgaaa tcgctccagt gcagcgtacc
     2821 gttaaacacc agatagagga tttcaacaga accgaccacg atatgggtgg tgtcacctag
     2881 ggcaataagc caggtcatca atataatcac cacaatcttt gccgcacctg ctgcaggaaa
     2941 catccatacc atagtggcga tcagccagcc ggaaatgatc gcgttggcaa acatctcgct
     3001 gggggtgttc ttcatcacat ccatgccgat tttgacaaat gcatcgcgag tttcttcatt
     3061 gaagataggc atatattcaa atgcccacgc agcaatacca gtcccgagaa tattacccag
     3121 cagcacgact ccccataacc gcataagtaa gccaacgttg ctcattgtcg gtttttgcat
     3181 gacgggtagt accgcagtca cggtgttttc ggtaaataat tgctggcggg ccattatgac
     3241 gataataaaa ccaaaggtat aaccgagatt ctccagtaag aagctgcccg gcacaccttc
     3301 cagttcgaca tgaaatatcc cttttgccag taacgaagcg cccatcgaca gacccgccgc
     3361 aatggctgac cacagtagcg ccattgcgtc gcgttccagc tctttttcac catcctggcg
     3421 gatatgctca tgaattgcca tcgcccggga ggggagtcgg tcttcatcta tttctatttt
     3481 tttgccgcgc tctttttctt cgctctcaac ttcaatttcg tcgctgtgtt gatcaatttt
     3541 gtcgttgtcc atggtctctt ctcttgaatt agcacgtata gctaaagcgt agcggctttt
     3601 ttgctcgcaa ctggcgggag ttactctgaa aatgtagaaa aggctgcgtt tgcctttttc
     3661 tgtttctata gaatcaagta gcctacaggg cggcgattac caggctatga tcaaatcagc
     3721 aaatcagggc gtctggacat cagttgacgt gctgttacaa tcgcccacac ctaaacaggc
     3781 ggatacggta tcgttccgtc atggatggca aactgcataa gccataaaaa aacagggaga
     3841 catttatgaa gcttcgcctg tcggcgcttg ctctgggaac tacgcttctg gtggggtgtg
     3901 cgagttccgg tacagatcag caagggcgtt ctgacccgtt agaagggttc aaccgcacca
     3961 tgtacaactt caacttcaac gtattagacc cgtatattgt tcgaccggtc gctgtcgcct
     4021 ggcgtgatta tgttccgcaa ccggcgcgta atggtttgag caactttact ggcaaccttg
     4081 aagaacctgc ggtgatggtt aactacttct tgcagggcga cccttatcag gggatggtcc
     4141 actttacccg ctttttcctg aacaccattt tggggatggg cggctttatt gatgttgcag
     4201 ggatggcgaa cccgaaactg caacggactg aacctcaccg cttcggtagt acgcttggtc
     4261 attatggcgt gggttatggg ccttacgttc agttaccgtt ctacggtagc ttcacgctgc
     4321 gtgatgacgg tggtgatatg gcggatggtc tttacccggt tctttcctgg ctgacctggc
     4381 cgatgtctgt gggtaaatgg acgcttgaag ggatcgaaac ccgcgctcag ctgctggatt
     4441 ccgatggtct gctgcgtcag tcgtccgatc cttatattat ggtgcgcgaa gcgtacttcc
     4501 agcgtcatga tttcatcgct aatggcggcg aactcaaacc gcaggaaaat ccgaacgcac
     4561 aagcgattca ggatgattta aaagatattg attctgaata agaaacaaat aaaaaaggtg
     4621 agtcgcaaaa ctcacctttt ttgttgttat ccctcaatta ccacaagtaa aaggagataa
     4681 accgacaatg acccggacgt aatgatgt
//
LOCUS       DAZPAC010000279         4699 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZPAC010000279 DAZPAC010000000
VERSION     DAZPAC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.281"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            387..1469
                     /locus_tag="ACHUPM_004032"
     CDS             387..1469
                     /locus_tag="ACHUPM_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR0871670.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="ACHUPM_004033"
     CDS             complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="ACHUPM_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFR0871671.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(2127..2267)
                     /locus_tag="ACHUPM_004034"
     CDS             complement(2127..2267)
                     /locus_tag="ACHUPM_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFR0871672.1"
                     /translation="MMFEFYMAEFLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="ACHUPM_004035"
     CDS             complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="ACHUPM_004035"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFR0871673.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2623..2913)
                     /locus_tag="ACHUPM_004036"
     CDS             complement(2623..2913)
                     /locus_tag="ACHUPM_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR0871674.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2906..3076)
                     /locus_tag="ACHUPM_004037"
     CDS             complement(2906..3076)
                     /locus_tag="ACHUPM_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFR0871675.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3076..3531)
                     /locus_tag="ACHUPM_004038"
     CDS             complement(3076..3531)
                     /locus_tag="ACHUPM_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFR0871676.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3796..4173
                     /locus_tag="ACHUPM_004039"
     CDS             3796..4173
                     /locus_tag="ACHUPM_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871677.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
BASE COUNT         1351 a         1048 c          977 g         1323 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagtcaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta
      301 catcttgaaa taatcacatt gattagatga atatttatcg cgcaatgaca tcatttttta
      361 ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      421 ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta
      481 acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct aatgatgcag
      541 atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc
      601 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag
      661 gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
      721 ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc
      781 tgccagaatt cggtggtgac acttggactc aaacagatgt gttcatgact ggtcgcacca
      841 ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      901 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt
      961 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     1021 ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     1081 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     1141 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     1201 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1261 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata
     1321 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1381 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1441 ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc
     1501 tttgcctccc caacatataa gtggctccct caagccactt cctttaggag cacaaccttg
     1561 cttctaacta tataaacctt ctgttatata ttacccttta tttttggggg cgttgcaacg
     1621 ccccattttt aataattttt agtaaacaat tggcatatta attagagtta ttaacaacga
     1681 tatccatctc taaccggata tctaatgcca ttaacatccc ttcaattatg ccctcagcct
     1741 tctgtaacct tttcccgata taaccatcag agcagcaatg cttacctgcc agtgacatga
     1801 atgtcatacc gactacataa taatctacta ataaatcgtg caaatcgctg ttgttctttt
     1861 tcagacgggc catgcacccg caaataatca tcgcgtcatc gtcacaacat tgcgggcgag
     1921 attttacttt tgaagtaatt aatcccttaa aaccggcggc aatggacgac caggtcacat
     1981 cttcatgatt attagccgcc cacgctcccc aacgctcaag aaccatctga atatcacgca
     2041 tcaacttact ccacaaaaat cagaccagaa cgccaattac aagcaaaaat caacaaaaca
     2101 gtattagttg attgttatct ctgacttcat actcctgctc ctgtcagggt tttggcgtaa
     2161 ttcttcagta ttcggtaatc ggtcaaaaca gaaccgggga aacgatataa gcgcagatgc
     2221 ccccagcggt ggcgaagaaa ttctgccata taaaactcaa acatcattca ttccccattt
     2281 cggtgatggt cagttccagc ctcccacctt tggtaacagg catcttcaca acgcggtaat
     2341 caacgacctg agcatcatcc agccagaaac ctgctttggt gagtgcgtca aaagcggctt
     2401 tttgcagatt atccaggtca cggcgacggc gatccggcat gtggcactca atacggattt
     2461 tcactggcat agccaggccg atatccagca ttgcgttttt aatgattcgg gtgacgttat
     2521 cgcggtatgc ctgcccttct gcgctgatgt gtgtgcgccc gcgattatgg cggtaatagc
     2581 ggttattgct cggaggccag ggtaatgtga tgctgtaggt attcacgcct taataacccc
     2641 ctctttcagc cagataacct gcgttctcgc cataccttcc agcgcgcatt cttttgcata
     2701 ctcagcatcg acaaaatatg tgcggcggtc gatttcgtcg tgacaggcag aacatgcaat
     2761 ggtggcaatc aggtctggcg gtttgatacc ggtaccgcac aatccagcca gccggatatg
     2821 tgccagtact gacgtttcag gattgccatt acatacgcca gggattctta cctggcattc
     2881 ccgaccacgc gctgcttttc tcaaatcagc catgattcct ccttgctgcc agtcgcaacc
     2941 attttttatc aaccaggcta gcggtatatc cgagcagtgt tggtatttcg gatggcttca
     3001 gctcaggctt acgcttacga cgatttgata ctctgtagat gtgcccgttc atgacacgaa
     3061 taagcggtgt agccattacg cctcctgctt atcgcgcagc tgctggtatt cacagctctg
     3121 cggaattgtc aggtggcagc cgatattcat cgcccaggct tcgactttgc acaggaagat
     3181 gtacatctcg ccggtttcca gctctgacgt atggcggagg gattggacgg tggtgacctc
     3241 gccggacacg acatctacac ggtctttgct ttcgtagccg agataggtgt gcttcatcgc
     3301 gtctttgacc cactcaggcg tagcgaaggt cttgccgcgg gcgatgagat attcgctgat
     3361 ctccgtgtac cacatgtggc tgagcgcgtt ctgcgacagg ctgcgcttct cgcgccacgg
     3421 cttaacctgc aggcgtaagc attgcccggc atccagcaat ggctgaatct gctggccgat
     3481 ggccgcgaag ttaccgcgat ggagtttgat gccgtctact ggcagagtca tacggcctcc
     3541 ctaacggaaa ccgcagaatg cagaaaatcg caggtgcatt tctgcatctg tgacaaggtg
     3601 atgagttcag attgtggtcg catttaagtc cccttaaatg cgcagaagtc accggagttg
     3661 ttcaggctcc gatgagagga ttatggcgga ttgattacga taaatcaaaa ctatcttgaa
     3721 gccaaaacag tgatcttcgc tttaaaaacc ttgtatactt tcacctaatc acagccagaa
     3781 ctatggatct aaccaatgac caacaaagaa aacaaagata aatatattaa caagttcaag
     3841 aaagagcttt caaaatatat acaaccaact gctggagtgg acattcaatt atttaactct
     3901 aaggatggtg gtggtgtaat taaagccact ttgaatagaa gtggaaaaag aaaatcatct
     3961 attgctggta attttactaa attaggtgag gcaattacct cgagtgggca gagggttttt
     4021 ggtggtgatc tatcaaatgt gaatttttac ggaacaaata cgatttttga tggagataca
     4081 atatttttaa ttaaatcacc tgattcagag gagtggtcat cacaaaaagc ttccaaagac
     4141 gtagagggca tcgtatttgg agggaaaaaa tgattaacat tactactaag gatgcactca
     4201 agtcagtggt gaagtaagag atctagtaat tcttcttgaa actgatggaa aaacggtgtc
     4261 tgattatttg cagccaatcc ctcccaaaaa agatacacac tggagcatga ttttcattcc
     4321 ggtagcaatt tatatactac ttaatcttgt atacattatt gctcacccat tatttcctga
     4381 aaaatatcta aaaacatcaa catctatatt tctattcatt tccttcatat taagtgcaat
     4441 agtagcatat tttgttttcc ataagcataa atctgccact ctatccatat gcagcttagt
     4501 gttcttagtg ttaattgtag gtgctaacgt agggtttatc agttacagcg acctaacaca
     4561 aaaagcgacg caaaaattag atagtttgtc taaataatta tgttgccatg cgaagtatgc
     4621 atggctgcat gtctgccttc catttaaaat gggctaagac ctataaccct aaatattatt
     4681 ctttattatc ttctttacc
//
LOCUS       DAZPAC010000280         4606 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZPAC010000280 DAZPAC010000000
VERSION     DAZPAC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.282"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..2162
                     /locus_tag="ACHUPM_004040"
     CDS             <1..2162
                     /locus_tag="ACHUPM_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR0871678.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2396..3679)
                     /locus_tag="ACHUPM_004041"
     CDS             complement(2396..3679)
                     /locus_tag="ACHUPM_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR0871679.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3814..>4606)
                     /locus_tag="ACHUPM_004042"
     CDS             complement(3814..>4606)
                     /locus_tag="ACHUPM_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0871680.1"
                     /translation="QKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKA
                     ASAKLIRSTLSDAFREAIAEGHITTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESS
                     PCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGI
                     SMKETLDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELR
                     SLSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         1093 a         1142 c         1194 g         1177 t
ORIGIN      
        1 aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat atggataaac
       61 ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt attgtacaga
      121 ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc aactgccata
      181 actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt ggtttatcgg
      241 cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc catcaatata
      301 tgcgtgagat gatggcaggc gggggcaaaa tgatcctcgg gtcagacagc cacacccgtt
      361 acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta aaacagctgc
      421 ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg accggaaaac
      481 ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg gtgttcaaaa
      541 acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc gcgctctcta
      601 ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta agctccgtct
      661 ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggc caggattact
      721 gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt gatttaagcg
      781 ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa atcgacacac
      841 tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa cgcgtggcgc
      901 acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg aaagtgcagc
      961 aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg gcggcgaatg
     1021 cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcagtttac ccgtcatcac
     1081 agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt ggcgcaggcg
     1141 caatcatcag aaccgcgttc tgcggcccat gctttggcgc gggcgatacg ccaatcaaca
     1201 acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc tctaagccag
     1261 ctaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct gcgactgcgg
     1321 caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac gtgccggagt
     1381 acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg aaaggggcaa
     1441 ctcagcaacc gctgatttac gggccgaaca ttaaagactg gccggaattg ggtgcgctga
     1501 ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtgacc accaccgacg
     1561 aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt ctggcggagt
     1621 ttaccctgtc tcgccgtgat cccggttatg ttggcagaag taaagcgact gctgagctgg
     1681 aaaatcagcg tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg cgcattaagc
     1741 agattgctgg tcaggagcat attgatccgc tgcaaactga aattggcagc atggtctatg
     1801 cggtgaaacc aggcgatggt tctgcgcgtg aacaggcggc gagctgccag cgtgtgattg
     1861 gcggtctggc gaatattgcc gaggagtacg cgactaaacg ctaccgttct aacgtcatca
     1921 actgggggat gttacctctg cagatggcgg aagtgccaac ctttgaagtg ggggattaca
     1981 tttacatccc tggcattaaa gcggcgctgg ataatccggg tacgacgttt aaaggttatg
     2041 tgatccatga agatgcgccg gtaacggaaa ttacgctcta tatggaaagt ctgactgctg
     2101 aagagcgcga gattatcaag gcgggtagtt tgattaactt caataaaaac cgtcagatgt
     2161 aaaaagcgcc atgtgaatgt aggtcgcatt cggcacttat tgtcggatgc gatgcttgcg
     2221 catcttatcc gacctacgaa tcgcatcgaa tctgtaggcc agataaggca tttacgcagc
     2281 atccggcact tattgtcgga tgcgatgctt gcgcatctta tccgacctac aaatcgcatc
     2341 gaaccgtagg ccggataagg cgtttacgcc gcatccggca aatagttaat tgctcttact
     2401 tcttcgcctc tgcaaccact ttactaccca cgccgcggtt attgtattcc cacatgcggt
     2461 tgtagttagt gtcattcaga ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat
     2521 tacccgcaaa cggacgatta gagatcaccg catcggccca cggtttagcc gtgttaaaac
     2581 cttcgttgat ggcgctatca cggatcacca cctgaccgtt ggtattggca tcaacatcca
     2641 gcgagcggcc cagttgcgcc acaccatcac cgaaagcatt gaaacggctg tttacggcga
     2701 ggaaaccgta gtacatgttg gacagcgtag ccggtgcaaa cacatacgct tcttgctgag
     2761 tacgtgagtt caccacgcgg aattcggtgt tatcgaacac cactgcgccg cgaccagaaa
     2821 cgatatccac atccccttca atgtagctgt tggtcaccag cgtacgcggc tgacgattcg
     2881 tttccagacg gttctgcaca ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa
     2941 tgttaacgtt gttaatctgt acctggtcac catcagtacg cagtgccacc gccggatggt
     3001 tacctgcatc tacgctatcg cccagcgtgt tttcgatggt cagattttgc agttgcaggc
     3061 cattgttttg tgaccagaag accgcagagc agagaacacc gatactgtcg ctgcgtttgc
     3121 tctggcagct atcgtacata taccacgctg gtttacctgg catatatttg ccgcgcgggt
     3181 tgacgtcgtg acgccagtcg gcagggctca tgccaccatc aagggaaagc ccaatcttca
     3241 catcaatcgg tttttcacct gtaccgtaca gagtaattcc acccggagcg gcagggacat
     3301 ataccgttcc ctgatactca ccaggcatca cggcaatata ctggcgcttg ttggtacgct
     3361 tgataattgc cgcatctacc gccgcctgaa tcgtggtatg cgttacacct tgagtgcccg
     3421 ccgggccgac aacaaagtca ggttgcgcag gcagggtaat cggggaagga ttccacgctg
     3481 cagcacctgg tgtcagggat gcaaaatagt gttgagcatc gaaattctgc gcttcttttg
     3541 ccgacagaat cgggcgagaa gaggtaccag gcgcggtttg atcagaagga cgttgatcgg
     3601 gcggggttga gctacaggcg gtcagcgtca cgccaaaagc caatgccagc gccagacggg
     3661 aaactgaaaa tgtgttcaca ggttgctccg ggctatgaaa tagaaaaatg aatccgttga
     3721 agcctgcttt tttatactaa gttggcatta taaaaaagca tcgcttatca atttgttgca
     3781 acgaacaggt cattatcagt caaaataaaa tcattatttg atttcaattt tgtcccactc
     3841 cctgcctctg tcatcacgat actgtgatgc catggtgtcc gacttgtgcc cgagaagatg
     3901 ttgagcaaac ttatcgctta tctgcttctc atagagtctt gcagacaaac tgcgcaactc
     3961 gtgaaaggta ggtggatccc cttcgaagga aagacctgat gcttttcgtg cgcgcataaa
     4021 ataccttgat actgtgccgg atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc
     4081 tccgccaaga atctctttgc atttatcaag tgtttccttc attgatatcc cgagagcatc
     4141 aacatgcaat gttgtaggga tggcaatttt tacgcctgtt ttgctttgct cgacataaag
     4201 atatccatct acgatatcag accacttcat ttcgcataaa tcaccaactc gttgcccggt
     4261 aacaacagcc agttccattg caagtctgag ccaacatggt gatgattctg ctgtttgata
     4321 aattttcagg tattcgtcag ccgtaagtct tgatctcctt acctctgatt ttgctgcgcg
     4381 agtggcagcg accggttttg ttgttatatg gccttcagct attgcctctc ggaatgcatc
     4441 gctcagtgtt gatctgatta acttggctga tgccgccttg ccctcgtcta tgtatccatt
     4501 gagcattgcc gcaatttctt ttgtggtgat gtcttcaagt ggagcatcag gcagccccct
     4561 ccttattgct ttaattttgc tcatgtaatt tatgagtgtc ttctgc
//
LOCUS       DAZPAC010000281         4579 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZPAC010000281 DAZPAC010000000
VERSION     DAZPAC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.283"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..754)
                     /gene="idnO"
                     /locus_tag="ACHUPM_004043"
     CDS             complement(<1..754)
                     /gene="idnO"
                     /locus_tag="ACHUPM_004043"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFR0871681.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGML"
     gene            complement(778..1809)
                     /gene="idnD"
                     /locus_tag="ACHUPM_004044"
     CDS             complement(778..1809)
                     /gene="idnD"
                     /locus_tag="ACHUPM_004044"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFR0871682.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            2026..2589
                     /gene="idnK"
                     /locus_tag="ACHUPM_004045"
     CDS             2026..2589
                     /gene="idnK"
                     /locus_tag="ACHUPM_004045"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR0871683.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            complement(2593..3612)
                     /gene="ahr"
                     /locus_tag="ACHUPM_004046"
     CDS             complement(2593..3612)
                     /gene="ahr"
                     /locus_tag="ACHUPM_004046"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR0871684.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            3808..3892
                     /locus_tag="ACHUPM_004047"
     tRNA            3808..3892
                     /locus_tag="ACHUPM_004047"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3842..3844,aa:Leu,seq:caa)
     gene            4078..>4579
                     /locus_tag="ACHUPM_004048"
     CDS             4078..>4579
                     /locus_tag="ACHUPM_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0871685.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARLQQR"
BASE COUNT         1194 a         1124 c         1082 g         1179 t
ORIGIN      
        1 ctaacatgcc gccatcaaca aacaacaggt ggccgtttac gaaatcagag gcttttgaag
       61 aaaggaacac cgcagcacca atcagttcct gcggatctcc ccagcgtgcg gcgggggtcc
      121 gtttgcacaa ccaggcggtg aaggcttcgt cctcaaccag tgctttagtc atttctgttt
      181 tgaaatagcc cggcgcaata ccgttgacct gaatattgtg gcgcgccagc tcgacgcaca
      241 tgccgcgggt gagcattttt accgcccctt tcgatgcggc ataaggggtg atggtgtcac
      301 gtcccagttc gctttgcatc gagcaaatat taataacttt acctgccttg cgttcaacca
      361 tgtgacgagt taccgcttgc gataccagga acacggcggt ctggtttact gcgatcacat
      421 cattccactc ttgttcaggg aactcagtaa aaggatgacg gcgctggata ccggcgttat
      481 tcaccagcac atcaatgggg ccgatgtcct tttcgatatg ttcaacggcg gcatcaattt
      541 catgtttatg agtaacatta aaaggtgcgg caacggcctg aataccctcc tggtggagtt
      601 tttctacagc aagttctgcg cgttcggcag taatatcatt aataattatt tgtgcgccat
      661 atttacccag gccggttgcc agtaaatagc caatgccctg tgctgaaccg gtaatcaaga
      721 tattttttcc tgccagtgaa aatagatcgt tcattgtatt attcctttat ttatctctta
      781 gaaaacaagc tggacttttg ctgcctgggt tttatcaccg gcgaaacgta gcgcctcttc
      841 caggtcagtg aagagatatt cagcactcag taaaggcagt ggattgataa cgccattcgc
      901 cagccatgac actgcggtat taaattcgct ggtaaaacgg aaagagcctt tgagtgaaat
      961 ctccttacca atcaacgtca tcattgggaa ttctgccatc gcgcctccca tccctacctg
     1021 caccattacg ccgcgtgcac gagtgacctc cagacaggta ttcactgatg aaggatgacc
     1081 ggacacttca aagctgacat cgaaatggcc tttttccgct ttccagtgat ccatgtcgtc
     1141 gttttgtggg tttacgagca catccgcacc catctctttg gccagcgaaa gggaacgggg
     1201 actcacatcg gcacagacaa tttccgcgga ccccagtgtt ttcacagcac tgacaatcag
     1261 gcagccaatg ggcccaacac cggaaacaaa tactcgcttg ccctgtaact cgtcggcctg
     1321 atgtgcggca tgaatcgcga cggctaaagg ttcggcaaaa gccataacct tttcgtcagc
     1381 tttggccgga taagggacac attgcgacgt ttcgaccatt ttataacggg taaaaccacc
     1441 atcaacatga gggaaataca tggcactgcc aaaaaaacgc atatctgtac actgattctc
     1501 gttatgttca atgcagtatt tgcagtgacc gcacggttta gacggattaa tggctaccgt
     1561 ttgcccttca tgtaattctg atgagtcgct atgaataact ttaccgataa cttcatgacc
     1621 taacaccatc ggtgccttta tcatgaaatt acctactttt ccttcctgat aataatgtaa
     1681 atcggaaccg caaattccac ctcgggttat ttgtactaat gttccattat tattccaatc
     1741 tatcgtctgc tcggtaacgg caacagtttt cttgtccgca acaacgcagg actgtgtttt
     1801 cacttgcata aattcctcac tggtcaggta gacacctcgg aagcatttaa gcggttttaa
     1861 ttgtcattat ttgtgatgaa gatcacgtca gaaaattgtt acattactat gttacgcata
     1921 acgtgatgtg ccttgtaatt cttatcagca gaaataaaaa aacgtgaaat tattatgcca
     1981 ccaggcgtag tatcgcagca ggtaagatga ttcaggagat tttaaatggc gggtgaaagc
     2041 tttattttga tgggcgtttc agggagtggt aaaacattaa ttggtagcaa ggttgccgcg
     2101 ttattatctg ctaaatttat tgatggtgac gatcttcatc cagccaaaaa tatagataaa
     2161 atgtcgcagg gtattccatt atctgatgaa gatcgacttc cctggctgga acgcttaaat
     2221 gatgcttcat acagtcttta taaaaagaat gaaacagggt ttattgtctg ttcatcatta
     2281 aaaaaacagt atcgtgatat tttacgcaag ggtagccccc atgttcattt cctctggtta
     2341 gatggcgact atgaaactat tctcgcgcga atgcagcggc gggctgggca ttttatgccg
     2401 gtagcgttac taaaaagtca gtttgaggcg ctggagcgtc cacaagcaga tgaacaggat
     2461 attgtacgca ttgatatcaa ccatgatatt gccaatgtca ccgaacaatg tcggcaggcc
     2521 gtgctggcga tacgacaaga cagaatatgt gcgaaagggg gtagcgcctc agatcagcgc
     2581 tgcgaatgat tttcaaaaat cggctttcag aaccacgcgg taacgcgcct taccgttgcg
     2641 cacatgctgg atggcgtcgt ttattttcga catcgggaac agttcggtgg tcggcgcaac
     2701 cttgctgcgg gccgcaaagc gcatcagctt acgaagctca taaggtgtgc cggttgctga
     2761 accagagacg ctgcggtcgc ccgcaattaa cgtaaaggcc ggaacagaca gcggcgtgag
     2821 gaccgcaccg acggtgtgga aattaccgcc atacgtcagc gcctcaaaat aaggctgcca
     2881 gtcgaggctg acgttgacgg tgttgataat gagatcgaac tgccccgcaa gtgttttcag
     2941 tgcctgcgga tcgcggctat tcaccacttt atcggcaccc atcgccagta cttcctgctc
     3001 tttcgcagga ttcgaactaa aggctgtcac ctcgcatccc attgcgtgca gaagttttat
     3061 agcgatatgc cccagcccgc caataccaat taccccaacg cggctggtag cagtgatatg
     3121 gtgcatcaac agtggtttaa agaccgtgat accaccacac agcagcggcc cggcggactc
     3181 gatatcgata ttttccggca gtgggatcac ccattgccag tctgcacgca acttctcggc
     3241 aaaaccgccg cgattcataa ttgttggcac cgcaccttgc tcgcagttga tctgattacc
     3301 gctaatacag gcgtcgcaat gcccgcagct acgcgccgtc cagccaatcc cgacacgctg
     3361 accgacctgc aaacctttat cctgcgcggc gctcccgagc gccaccacgc gcccaatcac
     3421 ctcatgcccg gcaaccagcg gatactgtga aaatccccat tcgttgtcga tcatcgacag
     3481 atcggaatgg cagatcccgc agtaatccac ctgcacttca acatcttgtg gcttcaattc
     3541 accgggctcg tactcataaa cttccagttc gccgcccgct tcttttgcgg catagctttt
     3601 tatcatcgac attttttggt ccttctctgg tgttgtctgg gaagtgtaga gcatggcagg
     3661 gcggggtgcc tggagtgtga caaaggttac acatcgctgt atgcaatgct gaaaatttca
     3721 gcacttagcg aggtgcgagc aagctggcgc ttgcatggtg gcgtgcgaca ggtataatcc
     3781 acaacgtttt ccgcatacct cttcagtgcc gaagtggcga aatcggtaga cgcagttgat
     3841 tcaaaatcaa ccgtagaaat acgtgccggt tcgagtccgg ccttcggcac caaaagtatg
     3901 tcaatagacc tcaactgagg tcttttttat gcctgaaatt cagtatttat ctacctttcc
     3961 cgctatatta actctctcaa ggtcaaccga tatcaacgta cttctaccaa catatgttgg
     4021 tacagatgat ggtatttccg gttcgataat gcttgtacca acagggaggg aatacgcatg
     4081 gcattaacag acatcaaagt cagaacagct aagccaacag ataagcaata taagttgact
     4141 gatggtggcg gtatgcatct gcttgtccat ccaaatggtt ctaagtactg gcgtttgcag
     4201 taccgttatg agggaaagca aaagatgctg gcacttgggg tttatcctga aatcacactc
     4261 gcggatgcca gaatacgtcg tgacgagcca cgtaagctgc ttgcgaatgg cgtcgatccg
     4321 ggagacaaaa agaaaagtga taaggttgaa cagagtaaag cacgaacctt taaagaagtc
     4381 gcgattgagt ggcatggcac caataaaaag tggtctgaag atcatgccca tcgtgtgcta
     4441 aaaagtctgg aagataatct ttttgcagcg cttggtgaac gtaatatcgc tgagttaaaa
     4501 actcgagatt tattagcacc tattaaggcc gtagaaatgt ctggacgtct tgaaatggct
     4561 gctcgtcttc aacagcgca
//
LOCUS       DAZPAC010000282         4575 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZPAC010000282 DAZPAC010000000
VERSION     DAZPAC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.284"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            10..333
                     /locus_tag="ACHUPM_004049"
     CDS             10..333
                     /locus_tag="ACHUPM_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871686.1"
                     /translation="MANDVGGVTHDQVERIAKIVAREVVGKLGKEIREEIVHEVHEQL
                     KNYFGDMTPAQHSIQHSNLDKLLNRLDSISSGFFGGIISKVTSFIITALILGLAAYGV
                     KNGLQ"
     gene            347..1039
                     /locus_tag="ACHUPM_004050"
     CDS             347..1039
                     /locus_tag="ACHUPM_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871687.1"
                     /translation="MKTPRGIRNNNPGNLDKGSPWQGLVANPDEPRFCTFKDPVWGIR
                     ALAVTLITYHDKRRAKDGSSIDTIREVIERWAPPNENNTDAYINEVSKAVGVTADMII
                     DLHDYDILRPLVEAIIRHENGRGPLKTLNTWYAAEVIEEGLRRAGVVKPVKTVKAVPV
                     TKETAGATVTAGIGLAQLADVMPQVSAAMDKAQGHISSGDTVRIIFGIATIVVAGFIA
                     WSQVRRHQKGMV"
     gene            1041..1292
                     /locus_tag="ACHUPM_004051"
     CDS             1041..1292
                     /locus_tag="ACHUPM_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871688.1"
                     /translation="MLGSLMTKLKVALITLAAVLFVLVGAYTMGGNAARRAMEEKAKQ
                     EDRKRLQSTVEVVNETSNKIRQKDASAVHRELYDKWVRH"
     gene            complement(1460..1981)
                     /locus_tag="ACHUPM_004052"
     CDS             complement(1460..1981)
                     /locus_tag="ACHUPM_004052"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0871689.1"
                     /translation="MDNIKIEIFSGEKDYDLNGFDCGEESLNTFLTNHLKRQHEGKIL
                     RAYVLCTKKGRPKVLGYYTLSGSCFEKESLPSRSQQKKVPYRNVPSVTLGRLALDKSL
                     QGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLDFIQLVGNNERSL
                     FYPTKSIEKLFEE"
     gene            complement(1989..2258)
                     /locus_tag="ACHUPM_004053"
     CDS             complement(1989..2258)
                     /locus_tag="ACHUPM_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004109769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR0871690.1"
                     /translation="MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERA
                     VKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKRAADRLKSME"
     gene            2577..3245
                     /locus_tag="ACHUPM_004054"
     CDS             2577..3245
                     /locus_tag="ACHUPM_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0871691.1"
                     /translation="MTKIFVVGGTKGGPGKSTVAQQIAVCLKVKKKKKVYITDIDIQR
                     TTTSWCEDRRQNEDLELIPFAYVQDDIIKHLKSLQGRAEFVVVDAGGFDSEIQRQAML
                     MADVIIIPLRPKRRDLKSLRDIDPIIDNVRNVNDKVKVRAVMNQCPALPSQVSRILAA
                     KEIVETFGIESAPVNLYNRNVYDDAEESGRSIFEMTGSERDKKAEAEFEEFVDYLLSL
                     EEEE"
     gene            3251..3604
                     /locus_tag="ACHUPM_004055"
     CDS             3251..3604
                     /locus_tag="ACHUPM_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871692.1"
                     /translation="MKMGDLAKRKEPDAPAKNTTPLRQPVRPQGRPTRGKEKIKSRTM
                     SLEDEYFELLEMMKFIPRFEKFTRSDVIRAAIFHLAEKSPQEIEDIVKLNEAITAADV
                     TMRTDEIKRELMKKG"
     gene            3656..>4575
                     /locus_tag="ACHUPM_004056"
     CDS             3656..>4575
                     /locus_tag="ACHUPM_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HFR0871693.1"
                     /translation="MESIESFLKNFLVSDHRVAVIKGDWGVGKTHYWNSFYTKHSEGL
                     DFNAYSYVSLFGVNSIGDIKKALYHCATPINEKKYKELILSETDRTMIRYRNGFWGWL
                     KYNSLSKFLIHFGKNDFFGFKTDNLLSSLEYKFVNNYLVCIDDVERKGNSLEVKEIMG
                     VIDELARRKGCKVVLILNEDNLHDETAKKQFLEYREKVIDVEIKYDPTPEKNLRKVFY
                     ETDSDFLLLKVLANDLGIKNIRILNKIKTSLVNLRNELSLAEDKVRESFINRLVLFSV
                     VYYSGVPGVDYALFKESIKNIHVFDHMLDD"
BASE COUNT         1376 a          874 c         1045 g         1280 t
ORIGIN      
        1 ggaggaagca tggctaatga cgtaggaggc gtcactcatg atcaggttga gagaatagct
       61 aaaattgtcg ccagagaagt tgttggtaaa ttaggaaaag aaatcagaga ggagatagtt
      121 catgaggttc atgagcagtt gaaaaactat tttggtgaca tgactccggc tcaacacagt
      181 attcaacatt ccaatctgga taaactactt aataggttag attcgatctc aagcggcttc
      241 tttggaggca ttatttctaa agtgacgtcg tttattataa ccgcactaat tttaggatta
      301 gccgcatatg gcgtaaaaaa tggactgcaa taacaggaga acaaggatga agactccgag
      361 aggcattcgt aataataacc ccggtaatct tgataaagga tcaccgtggc aaggtctggt
      421 tgcgaatcca gacgaaccgc gcttttgcac gtttaaagac cctgtttggg ggattcgtgc
      481 gctggcggtg actctaatta cctaccacga caaacgtcgc gcaaaagacg gctcaagtat
      541 cgataccatt cgtgaagtta ttgaacgttg ggcaccgccg aatgaaaaca atactgacgc
      601 ctacattaat gaggtgtcta aagccgttgg tgtaaccgca gacatgatca tcgatctgca
      661 tgattacgac atccttcgac ctttggttga ggcaatcatt cgccatgaga atgggcgagg
      721 tccgctaaaa acgctgaaca cctggtatgc ggcagaagtt attgaggaag gtctgcgtcg
      781 agctggagtc gttaagccgg tgaaaaccgt gaaggctgtt cctgtaacta aagaaacagc
      841 aggcgcaact gttacagcag gtattggtct ggcgcagctg gccgatgtta tgccgcaggt
      901 ttccgctgct atggataaag cacaaggtca tatctctagt ggggatacag tacgcatcat
      961 cttcggtatt gccactattg ttgtggcagg attcattgcc tggtcgcagg taagaagaca
     1021 ccagaaaggg atggtctaat atgctaggca gcctgatgac aaagctaaaa gttgctttga
     1081 ttacgctggc tgccgttctt ttcgttcttg tcggcgctta cacgatgggc gggaatgcgg
     1141 cgcgacgagc aatggaagag aaggcaaaac aggaagacag aaaacgactt caaagcacag
     1201 tggaagtggt gaatgagacg agcaataaaa tacgtcagaa agatgcttct gccgttcatc
     1261 gtgagttgta tgataagtgg gtgcgtcatt aaaccacaaa ccgccagcgt attgttctgc
     1321 gatggagctg ggcctatcta tatcagcaat aatgatgtga tgaccgaaga aaccgaacgc
     1381 caaatccttt ttcacaatac gatgggagag agagtttgcg gttggtgatg cgaagttccc
     1441 ctcacttgag gggagtacat tattcttcga acaatttttc gatagatttt gtaggataga
     1501 acaaagaacg ctcgttgtta ccaaccagct ggataaagtc taaactttta taaaacgcct
     1561 tggctttgtc atttaacgcc tcaacgaaaa gtccatgaat gcctacagca agagatgcat
     1621 tgtacacaac gcgcattgca tgtgttacaa gcattgagcc aaatccttga ccttgaaggg
     1681 acttgtccaa agccagccta cccaaagtaa cacttggaac attccgataa ggtaccttct
     1741 tttgctggct tcttgaaggt aaggactcct tttcaaagca gctacctgac aaagtgtaat
     1801 accctaacac ttttggtctg ccttttttcg tgcaaagcac ataagcgcga agaatttttc
     1861 cctcatgctg cctttttaag tggttggtta aaaaggtatt tagtgattct tcaccgcaat
     1921 caaaaccatt caaatcataa tctttctctc cagagaaaat ttctatcttt atattatcca
     1981 cgagtaaact actccatgct tttcagacga tcagcagctc gtttcagctt gtcgttcggt
     2041 gccggaggat tgcttatagc gtccataacc agattccagg attcttcatt cagaaccagt
     2101 ctacggtgtt gctctataac tttcacggca cgttcagatg cactgctaac cataaactga
     2161 gtaatgctct ggttagacat tgcggcagct tcctcgatga tgcttttatc gtcatcggtt
     2221 aatctcaaat cgatgcgctg cttttttagt gctgacatgt attcctcctg atggccgtga
     2281 tgtaggaagg ccatcaatct cctctgttca aactgagttt tcgactcaaa aattacttta
     2341 aaaggctata gcccattacg tgtgcattat caaattgtgt acagcatatc accgtacttg
     2401 taatatagag gtcattgtta cttttttcaa cagacaaata caagctatgt ttgccaattg
     2461 ttaatcacaa cctgccattt aacctttaca ccgcagccgt aggcatttag gctatatcac
     2521 atataagaaa acaagttgtt tcagacgata attatatacg caaagggaac tctccaatga
     2581 ccaagatctt tgtggttggc ggcacaaagg gcgggcctgg caaatccacc gttgcccagc
     2641 aaattgccgt ttgcctgaaa gtcaaaaaga agaagaaggt ttatattacc gatatagata
     2701 ttcagcgcac gacaacgagc tggtgtgaag accgtcgaca gaacgaagac cttgagctga
     2761 ttccttttgc atacgttcag gatgacatca ttaagcacct aaaatcgctt cagggtagag
     2821 ctgagtttgt agtggtagat gctggtggct tcgactccga aattcagcga caagcgatgc
     2881 tgatggctga cgttatcatt atcccgctgc gtcctaagcg tcgtgatttg aaatctttac
     2941 gtgacatcga tcctattatc gacaatgttc gcaatgtaaa cgataaagtg aaggtccgcg
     3001 cggtcatgaa ccagtgcccg gctttgccat cacaagtgtc tcgcattctg gcggctaaag
     3061 agattgttga gacgtttggc atcgagtctg cgccagtcaa tctatataac cgcaacgtct
     3121 atgatgatgc ggaagagtct ggtcgttcta tctttgaaat gaccggtagc gagcgcgaca
     3181 aaaaggcgga agccgagttt gaagaatttg tagattatct gttgagtctg gaggaagaag
     3241 aataatgtcc atgaaaatgg gtgacctagc aaagcgcaaa gagcctgatg caccggctaa
     3301 gaacacaact cctttgcgcc aaccagtcag accacaggga cgcccgactc gtggcaaaga
     3361 gaaaattaaa agccgcacaa tgtcactgga ggacgaatac ttcgaactgc tggagatgat
     3421 gaagttcatc cctcgcttcg agaaattcac ccgctctgat gtgattcgtg ctgctatttt
     3481 ccatctggca gagaagtcac cgcaggaaat cgaggacatc gtgaaactga acgaggcgat
     3541 cacagctgcc gatgtcacga tgcgtaccga tgaaatcaaa cgagagttga tgaagaaagg
     3601 ttgattatat tgggcgtttg ttacgcccaa tatatgaatt aaaggattga tactaatgga
     3661 atcgatagaa tcatttctaa aaaattttct agtttcagat catcgggttg ccgtcataaa
     3721 aggagattgg ggcgttggta aaactcatta ttggaatagt ttttatacta agcattctga
     3781 aggattagat tttaatgcat attcgtatgt ttctttgttt ggggttaact ctattggtga
     3841 tattaaaaaa gctctttatc attgtgccac accaattaat gaaaagaaat ataaagaact
     3901 aattttatct gaaacggatc gaactatgat tcgttataga aatgggtttt ggggatggtt
     3961 gaaatacaac tccttgtcaa aatttttaat acatttcgga aagaacgatt tttttgggtt
     4021 caaaacagat aacctgctgt catctcttga gtataaattt gttaataatt atttggtttg
     4081 tattgatgat gttgaaagaa aaggtaattc gctagaagtt aaagaaatta tgggggttat
     4141 tgatgaatta gcaagaagaa aaggttgcaa agttgttcta attttaaatg aagataattt
     4201 acatgatgaa actgcaaaaa aacagttttt agagtacaga gaaaaagtaa ttgatgtcga
     4261 aataaaatac gatcctacgc ctgagaagaa cttacgtaaa gttttttatg aaactgattc
     4321 tgattttctt ttgttaaaag tcttagcaaa tgacttaggt attaaaaata tccgtatatt
     4381 aaataaaata aaaacatctc tggttaatct taggaatgag ctaagcttgg cagaggataa
     4441 ggttagagaa agttttatca atagattggt tctgttttca gtggtttact attctggtgt
     4501 tccaggtgtt gattatgcct tgtttaaaga atcaattaaa aacatacatg tatttgatca
     4561 catgttagat gataa
//
LOCUS       DAZPAC010000283         4531 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZPAC010000283 DAZPAC010000000
VERSION     DAZPAC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.285"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(12..251)
                     /locus_tag="ACHUPM_004057"
     CDS             complement(12..251)
                     /locus_tag="ACHUPM_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR0871694.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(254..973)
                     /gene="sanA"
                     /locus_tag="ACHUPM_004058"
     CDS             complement(254..973)
                     /gene="sanA"
                     /locus_tag="ACHUPM_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR0871695.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1123..2007)
                     /gene="cdd"
                     /locus_tag="ACHUPM_004059"
     CDS             complement(1123..2007)
                     /gene="cdd"
                     /locus_tag="ACHUPM_004059"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR0871696.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2137..2832)
                     /locus_tag="ACHUPM_004060"
     CDS             complement(2137..2832)
                     /locus_tag="ACHUPM_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR0871697.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2829..3227)
                     /locus_tag="ACHUPM_004061"
     CDS             complement(2829..3227)
                     /locus_tag="ACHUPM_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR0871698.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            3466..4413
                     /gene="dusC"
                     /locus_tag="ACHUPM_004062"
     CDS             3466..4413
                     /gene="dusC"
                     /locus_tag="ACHUPM_004062"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR0871699.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         1209 a         1153 c         1167 g         1002 t
ORIGIN      
        1 aagagaaaat attaacgata gaaaataaga taaacaatca tgccgataga taaaattccg
       61 aagccgatat atgccaccat ataagcgaaa aagagtccgg gattttttgc tcgccagaca
      121 tacaccggtt ccggtgcatt tttaacccgt ttatcaatcg cgcctgcgcg ccagcctttc
      181 catgcttcgc ggaaacaaaa tatcgggata aggaaaaaaa cggcaacgac aaaaaactgc
      241 tgtacatcca taactacttt ccttgtttct tttgtaattc aagtaactgt tcgggtgtga
      301 cggcgggata cccctgcgca tcttccggca cctggtgcat agccggaata gggaccagcg
      361 gccctaaaaa acgcggttca cgtctaaaaa tataaaggtc agccagcgca ccgaaacggg
      421 cggcaaattc gcgaatacgt actgacagca tatctttcgg tgacggtacg gcataacact
      481 gagcctgaat ccccatatgc agcgcaataa ataatgcccg ctcacagtgg aaacgttggg
      541 tgataataat gaaatcatta gtatcgaaca ctttgcgtgt acgcacgatg gaatccagcg
      601 tacgaaagcc tgcgtaatcg agaacaatat ctgatgggtc gacaccagca gcgattaaat
      661 ctttgcgcat ggtcatcggc tcattataac tttgcaatgc gttatcgccg ctcagtaata
      721 gataatttac cttaccgctg ttataggcat taatcgctcc ttgaatgcgg tagcgataat
      781 actgattaat tacgccagta cgataatatt ttgctgtgcc gagcaccacg ccgacctggc
      841 ggtaggggag atcctgcaat tcgtcgtaga tataaggcgc ggttttccag ctcatccagc
      901 gatcgaggcc gagcacagtc aacagcagca agccgatcag gactaacagg ctgaggaaca
      961 cgcgctttaa catgcaggag actcgatcta aggaaaattt ccatcaggct actttacccg
     1021 ctggttttgc gcaagaaacg gcaaactgtt tgccgggaat ttcgacacca agagagggct
     1081 ggcaagccgg acaaggcgtt cacgccgcat ccggcaccag gcttaagcga gaagcacgcg
     1141 gtcgatactg tgacagccga gagctttcag cgttgcggag gtggcatccc actgaatcaa
     1201 cggcgcatcg gctttttctg ccagaaccgc gcgctggata tccgggtaat cataaccctt
     1261 gagattcaac agaattaacg ccccctgcaa cggtggcaga gtcgggttga atgcggcgtt
     1321 ttcagcgtag ctgccgctga aaatacgacc gtctttacat tccagcgcga caccgcttgg
     1381 cgacttactg taaggcatgt gcgaacggtt tgccgccgca atcgctgcct gagaaagcgc
     1441 atcacccgtc agcgcatagc cgtgatcctg ttcgtccatc agcagcgttt taatctccag
     1501 atctttcggc ccaaaggcat ccggcagata gtcacgcagc gcgtgtgcct cgcggcccgg
     1561 cagatgaata cgcagatcca gaccgctgtt cagttcattc ataaactgac ggcagtgacc
     1621 acaaggcgta tagttaacgg tgatggctgc aagcgctttt tcaccactca accaggcgtg
     1681 gctgatcgcg ctttgttcgg catgaacggt ttgctgcatt gttgcaccaa taaattccat
     1741 attggcaccg aaataccagg ttccgctcac accgcgcgca attgcgccaa cattaaaatt
     1801 cgacaatggc gtacgcgcac aggccgccgc cagtggaagt agtgcgaatg ccagcgcgtc
     1861 ttcgtccagc cccgttgcgc tcttcagcga tgagacttgc tccccggtca acaaagcggg
     1921 gaagtacttg tctgccagaa taggttccag tgcagattgc aagttatccg caagttgggc
     1981 aaaagcggtt tgaaaacgtg gatgcatggc gttgcctcat aatcatgtaa tgggataaca
     2041 gtttacgaac ccgttatggc tttatatgtg atctgaatct cattaattat gcaacgcgtg
     2101 aaacttacat caaaaatgcg cgacgcatcg caaattttag cccattactg ccagaataat
     2161 cgggaaaagg aacggtgcga tcagcgaagt aattatcccg cataacacca gcgccagcga
     2221 actaaatgca ccttcctgat aatccagctc ggcgcagcgc gccgtaccga gggcgtgcga
     2281 ggctgtcccc attgccagac cgcgcgcagc tttggtacga atacgcatcg cattaagcaa
     2341 tgtatggcca aataccgcgc cgaggatgcc gacgaaaatc acgcaaactg cgctgattgc
     2401 cggaataccg ccaatactgc cgccaaccgc cattgcaata ggcgtggtga ctgatttcgg
     2461 caggattgac gcggcgattt ccggtgaagc gcccatcaac aatgccacgg aagtccccgt
     2521 caccatcgca accacgctgc cgataaaaca gatggtgatg atcgatttcc agcgcgcgcg
     2581 gatctggtgt agctgctcat ataaaggata ggccagcgcg acgaccgccg gttgcagcag
     2641 atcgtttagc acttcgctac ctttaaagta gctgtcgtaa gagatgccgg tcagcattaa
     2701 aaaaggaatg atcaccacca ttgctaccag caacgggtta agcaggggaa acttataccg
     2761 tgccgccagt ttgcgggcgg caaaaaagac aatcaacgtc agcggtaatg accaccagat
     2821 attcgccatc attcttctga tcctttctga cctacgactt tacgttcacc gtgtaccagt
     2881 tgtgaactcc agctcaccac cagaaaaacc accagcgtac tgactgcaca ggaaaccact
     2941 accgggccaa actgtgcgcg gagcaaatca aaatattgca tgacgcctac gccaatcggc
     3001 acaaacaata gcgccatata gcgaatcagt acgtagcacc ccggattgac ccattttgcc
     3061 ggaagaattt gcaaggccag caggacaaac aggatcagca tcccgatgat gctgcccgga
     3121 atggttaccg gtagcaggga agcaatgaaa atgcctgcat acaggcaggc ataaatcagg
     3181 acgaaagcgc gtaaatattg ccagataatg ttcagtgtct tactcataat caacacccca
     3241 gatgagatgg attcatcata caattaattc gtaaaacgtg ctaccgatca cattaagaaa
     3301 tcagcgctta ttttttcgct ccccggcatt acgcaatgcg caacgaactc aggcatacat
     3361 caagcataga gagggattac agtttgcagc aatccctctt tgcagacctt taatccgttg
     3421 accataggcg ctaacatagc gcctcatttt tttccgggtg aagatatgcg tgtgttactg
     3481 gctccgatgg agggtgtgct tgactctctg gtgcgagaat tgctgaccga agttaacgac
     3541 tacgatctgt gcatcaccga gtttgtccgc gtggtggatc aactgctgcc ggtaaaagtc
     3601 tttcatcgca tttgccctga gctacaaaac gccagccgga caccatccgg tacgctggtg
     3661 cgcgttcagt tgttaggtca gttcccacaa tggctggcag agaacgccgc ccgtgcggtg
     3721 gaattaggtt cctggggcgt ggatctcaat tgcggctgcc cgtcgaaaac ggttaacggc
     3781 agcggcggcg gggcgacgtt actcaaagat cctgaactca tctaccaggg tgcaaaagcg
     3841 atgcgtgaag ctgtaccggc gcatttgccc gtcagcgtga aagtgcgtct gggctgggac
     3901 agcggtgaga agaaatttga aatcgccgat gcggttcaac aggctggtgc tacggagctg
     3961 gtggtgcatg gacggacaaa agagcagggt taccgcgcgg agcatattga ctggcaggcg
     4021 attggcgaga ttcgccagcg gctgaatatt ccggtgattg ccaacggcga aatctgggac
     4081 tggcagagcg cgcaacaatg cattgcgatc agcggctgcg acgcagtgat gattggtcgc
     4141 ggggcgctca atattcccaa cctgagccgg gtagtaaaat ataacgaacc gcgaatgccg
     4201 tggccggagg tggttgcttt gctgcaaaaa tatacccgtc tggaaaagca gggcgatacc
     4261 gggttatatc acgttgcgcg gattaaacag tggttgagtt atttgcgtaa agaatacgat
     4321 gaagcaacgg aattatttca gcatgttcgg gtgttgaata attcccctga tattgcaagg
     4381 gctattcagg caattgatat cgagaaactc taaaaaacca tagtgatgtt ttttgacaat
     4441 gtgttagctc tttatcaata ggccgcacgg ttgtttggtg gcgtcaaaat tgccgaggcg
     4501 ttccctgaag gccggggcag cccacatgga g
//
LOCUS       DAZPAC010000284         4482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZPAC010000284 DAZPAC010000000
VERSION     DAZPAC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.286"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(7..1068)
                     /locus_tag="ACHUPM_004063"
     CDS             complement(7..1068)
                     /locus_tag="ACHUPM_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR0871700.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1065..2462)
                     /locus_tag="ACHUPM_004064"
     CDS             complement(1065..2462)
                     /locus_tag="ACHUPM_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR0871701.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2617..3615
                     /locus_tag="ACHUPM_004065"
     CDS             2617..3615
                     /locus_tag="ACHUPM_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0871702.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="ACHUPM_004066"
     CDS             complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="ACHUPM_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFR0871703.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1229 c         1098 g         1063 t
ORIGIN      
        1 attgcattat ttttcgctgg gtgttttgcc tttttgcagt gtttctttca cctgaccgat
       61 aagctgacga cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg
      121 gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga agtcctgcac caatcccctg
      181 agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc
      241 gcgcaccagt tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg
      301 caaacggctg taatacccca gttcaatgcc atacagcgtg gcgatgcgat taattaaacg
      361 cagattgcgc caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat
      421 caacgttgat tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg
      481 ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga
      541 ggcataccag cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt
      601 ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc
      661 gcgttcatgg gcgcgctgtc gcaagcacca taagcactgc cactctgtta ccacagaacc
      721 tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg
      781 ggtttgccag gcattcattg tccactgtac accctgcccg acaacacttg cgccaaacag
      841 agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc
      901 cattaccgct tcaacttgcc cctcttcttc aggcgcttca tcgagcgtgg ccggggcaaa
      961 attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgatcgac
     1021 ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
     1081 cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc
     1141 atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg
     1201 ggcaaacgcg caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccga
     1261 ttaccgcgca atgccggaat tttctcgccg ttgacatcaa taatgccgct ggtggtcgcc
     1321 tgcaccgagg ccagccccag gcaatccata ctaatccctt caaacgccgc attttgccag
     1381 gcatcctgaa tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca
     1441 tggtccgctt tcgttgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga
     1501 aagagcgtgc gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata
     1561 tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg
     1621 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa
     1681 ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac
     1741 tcgccccagg tatcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata
     1801 tctcctggca agacaaagcg ccctggctga ataaagtgca gcccctgctg tttacagtgg
     1861 tggagataat cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggtgct
     1921 agcgggtcca gcccttcgca catcattcgc catttgaccg accattcgcc gcgctgacca
     1981 ttgagtaagc ccgtcatctg gcgtgaccag cttaaatagt cctgcgccag catcggcagg
     2041 tcgagcaacc attcgccggg ataatccaca atctccagat acagcgtgga ggtgtctttg
     2101 aagtggcgta gcagcgaatc gttcgattta aaacgtagcg ccaggcggat ttcactgacg
     2161 ccgcgcgtag gcgtcggcca ggcgggagga tcgccatata actgcgccag tccttcatca
     2221 taggtgaagc gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcaggcgc
     2281 tcttcacgca ccgcgcttaa cagcggcaag cgtgctccgg cgtgaatatt gagcaactga
     2341 ttgaccatcg cagtgataaa cgctgttttg ccgctgcggc ttagtccggt tacggcgagg
     2401 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
     2461 attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca ttcgtaagga tatgaatgtg
     2521 ggggccaggc cattgattat caactgcccg tgttgtcatc gacgggcgaa ctccataaga
     2581 tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt
     2641 gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaactaat
     2701 atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcagcca
     2761 aacaagctag cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc
     2821 cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca tgccatcacc
     2881 gccaaagctg aagaagaagg tttcgacgtg atattgcaga cgtcgcacaa cccggcagaa
     2941 gacttacaaa aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta
     3001 agttcgccag cggatgagtc attttttgcc caactcgata aatacgatat tccggtggtg
     3061 gtgattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt
     3121 ggcgacagca ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa tattgcctgc
     3181 ctgcatgctc cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagc
     3241 ctggctgcgc ataatattgc agtgcgtgat gaattgattg ttgacggtgg ttatacccat
     3301 gaaacagcac tgcaagccgc gagagaatta ttaagccagt caccgttgcc tgaggcggtg
     3361 tttgccactg acagtctgaa attaatgagt atttatcgtg cggcagcaga gaaaaatatt
     3421 gctattccgc agcagttagc ggtggtgggt tatagtaatg aaacgctgtc gtttatatta
     3481 acgcctgcac cgggcggcat cgatgttccg acgcaggagt taggtcaaca aagctgcgag
     3541 ttattattcc gcttaatttc tggaaaaccg tcaccacaga atattaccgt tgccacgcat
     3601 atgacgctga aataaatacc agctaccttg cctgaatgcc ggatgcgatg ctagcgcatc
     3661 ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc
     3721 cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc actgtctcct
     3781 tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc gttctggaaa
     3841 tcataaccgt aaaataaacc tacacggtta aaatcatggc cttcacgttc aatatcatcc
     3901 tgccagtccc agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg
     3961 ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttattg
     4021 cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt
     4081 tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggta
     4141 gtgttcagat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg taaatcgtca
     4201 gtcagtttca catcccagtc tggcgcgatt ttccagcgct gcatattcgc cgtgtcttta
     4261 cccggttcat caacgtagtg ataaccataa ttacggaaac cgccggtcag gccgaaactg
     4321 aaatcatcgt tttcgaggaa ctgataatgc acctccagct ccgggcgatc aaaccacgtt
     4381 ccacgtttac ccgcgctata atctaccggc ccttcctgat aataggccag agcaattctc
     4441 cacggcccgt tggcggcatt aaaatagact gaaggctccg cc
//
LOCUS       DAZPAC010000285         4411 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZPAC010000285 DAZPAC010000000
VERSION     DAZPAC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.287"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHUPM_004067"
     CDS             complement(<1..105)
                     /locus_tag="ACHUPM_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871704.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNV"
     gene            complement(173..2122)
                     /locus_tag="ACHUPM_004068"
     CDS             complement(173..2122)
                     /locus_tag="ACHUPM_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0871705.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="ACHUPM_004069"
     CDS             complement(2207..2542)
                     /locus_tag="ACHUPM_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR0871706.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="ACHUPM_004070"
     CDS             complement(2542..2898)
                     /locus_tag="ACHUPM_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR0871707.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="ACHUPM_004071"
     CDS             complement(2898..4394)
                     /locus_tag="ACHUPM_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR0871708.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1020 a         1315 c          970 g         1106 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agccatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagccttat c
//
LOCUS       DAZPAC010000286         4409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZPAC010000286 DAZPAC010000000
VERSION     DAZPAC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.288"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(21..1967)
                     /locus_tag="ACHUPM_004072"
     CDS             complement(21..1967)
                     /locus_tag="ACHUPM_004072"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR0871709.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SADMTFRVKPESLILTIFFLGLIP"
     gene            complement(2133..2216)
                     /gene="yqgH"
                     /locus_tag="ACHUPM_004073"
     CDS             complement(2133..2216)
                     /gene="yqgH"
                     /locus_tag="ACHUPM_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR0871710.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2369..3076
                     /locus_tag="ACHUPM_004074"
     CDS             2369..3076
                     /locus_tag="ACHUPM_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR0871711.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3182..3257
                     /locus_tag="ACHUPM_004075"
     tRNA            3182..3257
                     /locus_tag="ACHUPM_004075"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3215..3217,aa:Phe,seq:gaa)
     gene            3455..>4409
                     /locus_tag="ACHUPM_004076"
     CDS             3455..>4409
                     /locus_tag="ACHUPM_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0871712.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1149 a         1162 c         1057 g         1041 t
ORIGIN      
        1 gatttactgc tacgtcgccc tcagggaatt aaacctaaaa agaaaatcgt caaaattaga
       61 gattccggtt tgacacggaa agtcatatca gcgctatgcg catcctgggg gttcatcacc
      121 actgacggga aactctgctg gcggaacatc gctttttgta ggtctttgac gtcgaaactg
      181 acatacagat cgtgcatctc ctgacacaac tggcgcaggg tgtagtcgcg atagcgcacc
      241 ggatatttgt tataaacgct cggcaacacc tcagccagcg gcgagtcatc ctcaatatgc
      301 tgttcaaatt gcgccagcat cgccaccagt tgtgccagct tctcgtggct ttccgccgga
      361 gttaataaga acagaatgga gttgagatcg cacttctccg gcacaatgcc gttctcacgc
      421 agatagtgcg ccagaatcgt cgccggaacg ccaaagtcgc tatattcgcc ggtttcggca
      481 ttgatacctg gtgtagtgag taacagcttg cacggatcaa caaaatactg atccgcggca
      541 tatccttcaa agccgtgcca cttcgccccc ggctcaaaac tgaaaaaacg gcggtcgctg
      601 gctaacacag atgtcggata atcctgccac aatttgccat caacaacggg cgggataaac
      661 gggcggaaca gcttacagcg cgcaagaata gccttgcgcg actcaatacc caactcaacg
      721 cactcagccc acagccgacg cccactctcc ccttcatgaa ttttggcgtt aacatccagt
      781 gcagcaaaca gcggatagaa agggctggta gaagcatgga gcataaaggc gttattcaac
      841 cgcttatgcg ggcaaaaacg cgcctgtccg cggatatggt tatctttttt atggatctgc
      901 gacgtctgtg agaatcccgc ctgctgtttg tgcaccgact gagtcacaaa gatccccgga
      961 tcgttttcgt taagttctaa tagcagcggc gagctatccg ccatcatcgg gataaactgt
     1021 tcgtaaccga cccacgcgga atcaaacaga atgtaatcac acagatgccc aacggtatcg
     1081 atcacctgac gggcgttata gacagttccg tcataggttc ccagctgaat aatcgccagg
     1141 cgaaacgggc gcggcagctc ggctttttct ggcgcaacgt cgcgaatttg ctggcgcaga
     1201 tactcttcat taaaacagtg cgcatcaata ccgccaatga aaccaaacgg gttgcgtgaa
     1261 gcttccagat agaccgacgt cgcccccgcc tgaatcagcg cgccgtgatg attcgactta
     1321 tggttgttac ggtcgaagag caccagatcg ccacgcgtta acagcgcatt cgtcaccact
     1381 ttattcgctg ccgatgtgcc gttcagcaca aaataggttt tatcggcatg aaagactttg
     1441 gctgcgaatt tctgcgcatc cttcgccgat ccttcatgaa taagcagatc gcccaatttt
     1501 acgtcagcgt tacacatatc ggcgcgaaag acgttctcac caaagaaatc gtaaaaatgg
     1561 cgtccggcag gatgcttttt aaaaaacgca ccatgttgat gtccagggca agcaaaggtg
     1621 ctgttgccca tctcaacgta ctgcgtcagc gtgtcataaa acggtggcag caaattctct
     1681 tcatactgac aggctgcgga ttccagctcc agccactgct gctcgttgcc gttgattacc
     1741 gccgtaacgc ccgcaggtaa ttcaacagca tgttcggaat acaaaaacac cggtagatga
     1801 aaaccggtgc gcttaagcaa cgcaagaatg ccactgcgac tatccgcagc ggtaatgacg
     1861 actgccgcga catccgtaaa atcagtatct cccaacgcca ccacgcggcg atgagtagaa
     1921 agtcgggata ccagttcact actggcggca atattcattg atttcataag cgcaaaaccc
     1981 gtttcgggga agtaagaata ccggacaaag tgtaaaaccc tgccccatga gatatgggtc
     2041 aaactggtca ccagctccga cgaccgccag acatcagtaa aagcagaaac gctctgattt
     2101 tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca tgagcagtaa catagaaagg
     2161 gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta aggagatggc gtgcatcggg
     2221 caaactccgt acagagagga gaaaattcgc gcaatcatgg cacctttcgc taaggcgtgc
     2281 aagccaggac tttgcgacca gacaagccat caacaaggca aaaagttcat aataagaaaa
     2341 tcaaacaata cagctaacag gaactcttgt ggtcatcggc ccttttatca acgctagtgc
     2401 agtcttactg ggtggcgttc tcggcgcact gctcagccaa cgcttaccgg aacgtatccg
     2461 cgtctccatg acatctattt ttggtctggc atcgctgggg attggtattt tactggttgt
     2521 gaaatgcgcc aaccttccag cgatggtttt agctacgcta cttggcgctc taattggcga
     2581 aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc aaagcacaaa atctgtttcg
     2641 tcactcacgt aagaagccag cgcatgaatc ttttattcag aattatgtcg cgattattgt
     2701 cctgttttgc gccagcggca ccgggatctt cggggcgatg aacgaaggga tgaccggcga
     2761 tccgagtatt ttaatcgcca agtcatttct tgatttcttt acggcgatga tcttcgcctg
     2821 ctcgctgggt attgcggtgt cggtaattag tatcccatta ctgatcatcc agctaacgct
     2881 agcgtgggct gccgcgctga tattaccgct gaccacaccg tcgatgatgg cagacttcag
     2941 cgccgtaggc ggtttattgc tgctggcaac cggattacgc atctgtggca ttaaaatgtt
     3001 cccggtggtc aacatgcttc cagcactcct tctggcaatg ccgctttccg ccgcctggac
     3061 cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt gatgatagat tgtgcagtct
     3121 gcagtaaatt gaagaaattt gattgacgag acgaggcgaa tcaggtttaa tgcgccccgt
     3181 tgcccggata gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc ttggttcgat
     3241 tccgagtccg ggcaccaaat tcatatcaac ggacctccac ggaggtccgt ttttcgtttc
     3301 agaacgccac gatttaagcg ttctgcctac aaatcaattc taccgaactc aaccagattc
     3361 cccccacatc aaccccattg tgtgggtata attgcgggca taccccagtt cgacagaatt
     3421 tgtaccctct ttaacgcatt cagaaggctg aactatggca ctgactgacg caaaaatccg
     3481 ggctgcaaag cccactgaca aggcttataa actcactgac ggggctggca tgttcctgct
     3541 ggtacatcct aatggctccc gttactggcg tctccgttat cgtattctgg gtaaggagaa
     3601 gactctggca cttggtgtgt atccagaagt ttctctctcc gaagctcgta caaaacggga
     3661 tgaggcccga aaactgattt cggagggggt tgacccttgc gaacagaaaa gagctaaaaa
     3721 agtagtccct gatttacagc tctcttttga acatattgcg cgacgctggc atgccagtaa
     3781 taaacaatgg gcacaatcac acagcaataa agtactcaaa agccttgaga ctcacgtttt
     3841 cccctttatc ggcaaccggg atatcacaac actcaatacc ccggatctgc ttatccctgt
     3901 tcgtgctgca gaagctaaac aaatttatga aatcgccagt cgtctgcagc aaagaatatc
     3961 tgccgtaatg cgttatgccg tacagtctgg catcatcaga tataatcctg ctctggatat
     4021 ggctggcgca ttgactacgg taaaacgcca gcatcgcccc gctcttgatc tttctcgcct
     4081 gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa cctgtcaccc agcttgccgt
     4141 tatgctgaat ttactggttt ttattcgttc cagtgaactc agatacgccc gctggtctga
     4201 aattgatatt gacaatgcca tgtggactat tccagccgaa cgcgaacccc tgccaggcgt
     4261 aaaattctca caccggggct ccaagatgcg aacaccacat cttgtgccac tcagcaaaca
     4321 ggctgtagcc atactgacag aactacagac atgggctggt gaaaatggtc tgatctttac
     4381 gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAZPAC010000287         4408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZPAC010000287 DAZPAC010000000
VERSION     DAZPAC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.289"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..590)
                     /locus_tag="ACHUPM_004077"
     CDS             complement(<1..590)
                     /locus_tag="ACHUPM_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR0871713.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="ACHUPM_004078"
     CDS             complement(583..993)
                     /locus_tag="ACHUPM_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR0871714.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="ACHUPM_004079"
     CDS             complement(999..1532)
                     /locus_tag="ACHUPM_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR0871715.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2598)
                     /locus_tag="ACHUPM_004080"
     CDS             complement(1510..2598)
                     /locus_tag="ACHUPM_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR0871716.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLVLGENILSCDTERSIRERFSEYQVSGQRPG
                     NDDDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRE
                     TTYTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGP
                     ADAYLPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2595..3986)
                     /locus_tag="ACHUPM_004081"
     CDS             complement(2595..3986)
                     /locus_tag="ACHUPM_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR0871717.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRKSLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4033..>4408)
                     /locus_tag="ACHUPM_004082"
     CDS             complement(4033..>4408)
                     /locus_tag="ACHUPM_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871718.1"
                     /translation="TSADNDVDLDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIG
                     VFDFANNASPATMTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVN
                     VNGESGVTYENCKLNMKDINDD"
BASE COUNT         1056 a         1286 c         1041 g         1025 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcac
     2041 cagcaccagc gccgtcgccg ctttacctgt tccgggtttt cccagaacca gtcgcccgca
     2101 ttcatcgtca taagccagcg tctgaacctg ccccagtaac cggttaagac aatcggcaac
     2161 ggtttcgccg tgttccggct gggcatcaat caccgctgtc tgcggcacgc cagcatcaac
     2221 aacggtgatg ccaaatggcg cagccagttc actgactatt ctgagcaggt tttttccgct
     2281 ctgctggagt ggcaaagcag agcagtcaac cagatcggct gttttgcttc gcccgacaat
     2341 tcccatgctg acgctgctgg cgtcataacg aagcggtagt gcctccacat atccggtgag
     2401 cacaggctca tccccgataa gcacttcaac cagctcacca ttttttatcc ggggttgata
     2461 atcccggctt ccgggccagc gggtggtaat ggcaacatta aaatcccggg caatacggtt
     2521 aatgcccgca ctgatacgga cggatgtcca gccgccccat tcgcgaccgg aaacccgaag
     2581 taaaacggta tcattcatct gacgggtacc cttaatgccc tgaccggaac aaagcccgga
     2641 tgagagatgg catttcgatc caggatatca gtttcacggg acgcatcgtc gtaccatgac
     2701 gcagccagaa caagcgcagg cagaacttcc gctggcgttc gctctgcggt ttcctccgtc
     2761 tgaaccagac gcgcctgaat atcccggttc agttctgtac gtaatgacgt cagctgaaaa
     2821 aacagcctgt catccgccgt acgcctgagc tcctgttcaa tcgccgcgtt cagcgatttg
     2881 cgtatgatgg tgagattttc ccgcgtgggt ggttgcgctc tctcatcctg tcctgtgctg
     2941 gctgccacgc tgtcaagtgc cggatgagaa acgtgaataa tatcggactg acgttcagtg
     3001 gaaccaccaa cagccacaac cgcctgctga tttttcacca ggcttccggg ttgtggcagt
     3061 gaagtgaccg cccttgtggc ttcgctgatt gccgtcgtcc ggatgacagc agccacaagg
     3121 tttgtctgct gtttttgcct gataaccgat gcggaatctg tgggccacac cgcacgcggt
     3181 gccagtcctg ggtccagcgt aatgccggac attgtcgtta tggactgcac cagatcctgt
     3241 gtgttatcca ccagccgggt tccggcccgc caggtatcct gcaacgtatg cacaaaatca
     3301 ctggcaaccg acggcggcat cagaatgacg gataaatcgc cctgcagtag tctcgctccg
     3361 gcagaaacag ccgaattaac cattttgaaa gctgtctgaa cggttcccag catatcggtc
     3421 gtccgggcaa taacatcgtt ctgaataaag tccggtattc ccgcgagatc aaaatcgccg
     3481 aacatgtctt caatcaactc atccaggaat acggacgact cctccagttt tctggcggtt
     3541 gcagctccgg cgaccggaaa tgacagttcc ccgctctcaa caaactgaaa tgaaacccgg
     3601 cacatacggc cttcagtgcc ggagtgagaa acggtcacct gcccgtcaat acagccctgc
     3661 atttcaccga actgcggatg gatcagcgtc cccggcccgg cggtttcaat cgctgtaatc
     3721 agcctgtcgc gctgctctgc gtaatcatca ccaacgagat acgcattaat cgtcagccgt
     3781 cgcgtggcac gcccgagatc ttccgtgtac ggtttgtcac ggtttgggta ttcatggacc
     3841 tgaacgcggc gtccgaacga accctcgtcg ctctccaccg aaaacggaac gccacgaaat
     3901 gaagcgtcgt aaagattatc gcgccaattg atacctgaag atgcagaaaa gagtgaagaa
     3961 agagaaggta aagaaggtaa gtccatactg tctccactat aaaaaaaccg ccaatttctg
     4021 gcggcttata tattaatcat cattaatatc tttcatattc aatttgcaat tttcgtaggt
     4081 cactcccgac tcgccattaa cattaactcc agctctgata ttcccgtttt cttttacatc
     4141 tacaaaaaac tggcctcttg gcatcttgaa cgcaaatgta aaaccaacag tatctgaatc
     4201 aaaatcatca tctttatata ttgggatcaa aaatgtcatc gtcgctggtg atgcattgtt
     4261 tgcaaaatca aatacaccta tacttttgcc gaattcataa gaagcttttc tcaatataat
     4321 cgcaccgctg gcagaagggg atgggcacga tatcctcagt tgtctgaatc catctgtatc
     4381 caagtcaacg tcattatctg cagatgtt
//
LOCUS       DAZPAC010000288         4389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZPAC010000288 DAZPAC010000000
VERSION     DAZPAC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.290"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            188..439
                     /locus_tag="ACHUPM_004083"
     CDS             188..439
                     /locus_tag="ACHUPM_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFR0871719.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(470..676)
                     /locus_tag="ACHUPM_004084"
     CDS             complement(470..676)
                     /locus_tag="ACHUPM_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871720.1"
                     /translation="MTIAERLIQKGFDEGFDEGFKEGFKKGALEVAREAACRLRDMGW
                     TPERIQEAAGLSGEELKKLFPDEQ"
     gene            complement(738..1640)
                     /locus_tag="ACHUPM_004085"
     CDS             complement(738..1640)
                     /locus_tag="ACHUPM_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR0871721.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKITGGKDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGALEKALAIACQLQK
                     MGMTPEQIKQATGLSDDELKKIIH"
     gene            complement(1637..2026)
                     /locus_tag="ACHUPM_004086"
     CDS             complement(1637..2026)
                     /locus_tag="ACHUPM_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFR0871722.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
     gene            complement(2203..2469)
                     /locus_tag="ACHUPM_004087"
     CDS             complement(2203..2469)
                     /locus_tag="ACHUPM_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871723.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(2563..2997)
                     /locus_tag="ACHUPM_004088"
     CDS             complement(2563..2997)
                     /locus_tag="ACHUPM_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFR0871724.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPSGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            3287..3513
                     /locus_tag="ACHUPM_004089"
                     /pseudo
     CDS             3287..3513
                     /locus_tag="ACHUPM_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3729..4229)
                     /locus_tag="ACHUPM_004090"
     CDS             complement(3729..4229)
                     /locus_tag="ACHUPM_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFR0871725.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRRARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
BASE COUNT         1014 a         1246 c         1179 g          950 t
ORIGIN      
        1 cacaacaatg gcatgtggtg gtcactggca caccactccc gcacaggtga aaacagagaa
       61 aaccgcacga catttacaac caaaaaaagt gaaatttctt cacttttttg cacttgttta
      121 tcgcgctgat ttggcaattg tgatgataaa ataaagtgaa gttttttcac tttttcaaag
      181 gtgttttatg actggctggg aactgcgaat ctggcgaaaa agcatgctct ggtcacgcga
      241 aaaagcggcc agagaatttg gcgtgacgca gagaacatgg cacgcctggg aaaatgcgga
      301 acaggtggac gtcaccgtct ggaggaccac ccaggcactt tcagtccgtg acctgctgcc
      361 tcacatgcag gggatgcgca aggcagatat catcaggcgt ctggaaaatg aactggggga
      421 aacggcagag gatgtgtgag cgtgattcac agcctggcag aatgccaggc tactgctcat
      481 caggaaacag ttttttcagt tcttcaccgg aaagtccggc tgcctcctga atccgttccg
      541 gcgtccagcc catatcccgc agccggcagg ctgcttcccg cgccacttca agcgcacctt
      601 tcttaaaacc ttctttaaaa ccttcgtcga aaccttcgtc gaaacctttc tgtataagac
      661 gttctgcaat cgtcatactg ttttctctgc tcctgctggc aaaaggcccg gtattttgcc
      721 gggctgtgag gttctcatca gtgaatgatt ttcttcagtt cgtcatcgga aagtccggta
      781 gcctgcttaa tctgctccgg cgtcatcccc attttctgaa gctggcacgc aatagccagg
      841 gctttttcaa gcgcaccttc catcctgccc tcctggcgac ctttttgctc aagacgttct
      901 gcgatagtca tcagtttatc ctcatgctgc ggcagacgct cagccagcgc ccgcataaat
      961 tccggtgata cagagtcccc tgcgttaacg atgtaatgaa tcagaacacg cacctgctcg
     1021 tctgaaattt ccaccatgac ctgcgggagt ttgtccagca gctccatcat gtcacgatgg
     1081 cggatatgct tcatcagcag cgtcagtgcg gccatccggc gatggttcat gatttcattg
     1141 tcatcaacga cagtgatatc aaccagcgga aacggctttg tgtatatttt agccgcaagc
     1201 gccggattct caaaacagtc cagccagttc atgctgtacg gatacgggct gcgctcacca
     1261 tgatagaaca gcaccggaat caccagtggc aacgttttgt gctgctccag atgtctctgc
     1321 atcgccgcca cactgtaacg taacatgcgg aaaggcatgt acagattttc ggtgctctgg
     1381 tgctcgatga gcgtataaac atagccgtct ttacccccgg tgattttcac gctccagagg
     1441 atatcgctgg cgtactgatg caggtccggc tctacaaagg tggcgggttc cagcttcagc
     1501 gtggacaggt cgcacaactg ccggtactcc gcaggaagat gcagttccag aaaatctctg
     1561 gcgacgtcgg gattcgccag gaacgaccgg aacgcggcat catgcggcgt gggcgtcgtg
     1621 gtgtttttct tcttactcac ggttcctcca tcaaatcagc agcatcgcag caatcatcgc
     1681 ccaaatcccc acaaccgaga ggacagcaat tgcgttcaaa gccatgacaa caatagccgc
     1741 gttctgagtc acggcgtatt tcttcagcaa tctcttctgc ggaaaggtaa tcaaactcct
     1801 cacgggccag ttccaggacc agcagatgtt gttctgtcgg caatgtggcc acaaaattat
     1861 caatagcagc actcaaacgt cgctgatata gctcataaac aagaccacaa cggggcatta
     1921 gttcttcgga taatagttcc agttttgagc gaattctgtc agcgttgaaa gtgacttcat
     1981 caaaagacat atgaaaagct ttgtcagtat caaaacaaga cttcattgtt atctccagcc
     2041 ctcccgggcc ctgaatgaaa gagaatctgt ttcccttttc tgttttttgt ggagacacca
     2101 tgcaacacag acgtcgtgat gtttttcatc ttgctcacgg ttcctcctgg taaagtacct
     2161 gaacacatcc tgccacctgc acaggcagac ggcaggggtt aatcagtgac agacccgttc
     2221 aatttcaagc gcgcggagca ggtggcccca ttcgctgact ttcatggcgg ggatacgtac
     2281 ccgcttctgc tcactgcagc gtctggcgat atgaccgctc atcgccagga cgtccgggtt
     2341 aatctcatcc tgtgccagct cctgacgtct gaagcgggta tggcggtgca ggggtttatt
     2401 ctcccgcagg cactcttcac gcagacgctt tacatactcg gcggttgcga agataattga
     2461 tggtctcatg gcataatctc catagatagt gattgagatg ccgggcttcc tggcggggaa
     2521 gcgccggcgg agagaggcag ggtttatcgc cctgcctttt tttcatttcg gacagcggcg
     2581 cagtgccagc gcgagcgctt tcatcttcca gccttccggc gtgcgataca tcaccttcca
     2641 gtagccgtcg atgaacacat aatggaagcg cacctcttca gtgctttcag tggcaatagc
     2701 ctccatgaaa gaacggtact cacgcgcctc actgtccatc cggaaaccac tgtcacgtcc
     2761 gtaataagcc gtcacaccgt ccaccgggtt ctgaagcgta tgcccggagg gtttgtcgca
     2821 acggggaccg aggcaacgga tatcaccgtg ttcaaccagg gcttcggcct gctgaccagt
     2881 ggcatagtgg gtatgcagga tgcgaccgtt ccaggtcggc tcaccgtgca ggtggcagta
     2941 aacggtacgg aacaccccgt cgcggcaaaa aacggaaatc gtgctgctga ctgtcatgaa
     3001 aacgtctcct tctgctgaat caggtcgccg ggccgaccgg ttgctccccg gaggggcgaa
     3061 agccccttct gccgtccact tcttcggggt ttctccccgc agtggacctt tccgatgggg
     3121 cgtccgtcac cacgtcacgg ggcagctgtc cgggggcgga cgcaaaattt tttgccgggt
     3181 ttatggctaa aaattttgtg gacggtatat cttcccctgg actgctgaaa cgggatgtgg
     3241 ttactccagc ccccggaaag tccacagcgg gaaacaggcc gcaggggtgg cggcaggagg
     3301 gaggactgct gcgccatcag gcgcagtttc ctcacacacc gtcaggtgtg cagggagcgc
     3361 aggtcacggc gcgacagggg cggcgcgacc ggaacggtcg ctgcaagcct ggtttttcag
     3421 acatcggacc gtagcggcag cccgaaaccg cgccagcggg ttcggcggag actggcgggt
     3481 gcgccagcag ctgcctgtcg gagtcgggcc tgacgaccgg atgccgccag gcagccggga
     3541 gccgaaggtg tccccgcctc cggaaatcag aacaacagaa acgacataac agaaaaatgc
     3601 ctcacaccat gacgggcagg aagggcagcg ccgcaggcgc ggccctcctg catttcaggc
     3661 aggcgtaagc ctgccagggt tttccggcgg cctggcaggc catacccccg ccggagcggg
     3721 ggagtaaatc accgacggaa cacatgaccg tcaataaagg tgaaggagtc caggaataaa
     3781 tcccgcgcat acgcctcata gtcaaaatag agcgccaccg tctccggcac gtcagccagc
     3841 aggccggtat cactggcgaa ctcaaccgca aaagcctcct cactgtcagc ctcgccccac
     3901 caggcatcgc ggaaggtgtc aaaatccgtc tcaccggtat catccaccca gagacgataa
     3961 gcctcttcgc agccttcatc ccgtgcccgg cggaagcctt caacccaggc ccagttgata
     4021 tgacattcag aggccatatt ccccgggaat ccctcataat cctgaaacat cagttcagga
     4081 tcggcttcat cctggtgaag agcacggcag gcggcgaaaa agtcgcgctc atcatcaaac
     4141 gtggtcaggt caaaccagcg tccggcgatg cttccacagt tgtatttgtg ccaggttcca
     4201 acgtatacag caggtgcaac aacagacatt cccgaatcct ccatagtcac atgttctgcg
     4261 acaccgggcg gtgccgtgcc tccataaaag ccgggcgata aggcggggat gcaggggcgc
     4321 aagggaagcc gcagcctgac gcacacggct gacgggagtt gcagggtgag gccgcgaaac
     4381 ctgagcgga
//
LOCUS       DAZPAC010000289         4378 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZPAC010000289 DAZPAC010000000
VERSION     DAZPAC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.291"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1326
                     /gene="xanP"
                     /locus_tag="ACHUPM_004091"
     CDS             <1..1326
                     /gene="xanP"
                     /locus_tag="ACHUPM_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR0871726.1"
                     /translation="IYRLEDRPPLPQTLFAACQHLLAMFVAVITPALLICQALGLPAQ
                     DTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQGTSFNFVAPLIMGGTTLKTGGAD
                     VPTMMAALFGTLMLASCTEMVISRVLHLARRIITPLVSGVVVMIIGLSLIQVGLTSIG
                     GGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNPYLRVASLVIAMAAGYALAWFMG
                     MLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLMLVFMITSLETIGDITATSDVSEQ
                     PVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCFGQNNGVIQLTGVASRYVGFVVA
                     LMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIAASGVRIVSREPLNRRAILIIAL
                     SLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGITAIVLNLIFPPEKQ"
     gene            1447..3156
                     /locus_tag="ACHUPM_004092"
     CDS             1447..3156
                     /locus_tag="ACHUPM_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0871727.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(3208..>4378)
                     /gene="yicI"
                     /locus_tag="ACHUPM_004093"
     CDS             complement(3208..>4378)
                     /gene="yicI"
                     /locus_tag="ACHUPM_004093"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR0871728.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT          968 a         1198 c         1174 g         1038 t
ORIGIN      
        1 atttaccgtc ttgaagatcg tccgccgctt cctcaaaccc tgtttgccgc ctgtcagcat
       61 ctgctggcga tgttcgttgc ggtgatcacg ccagcgctat taatctgcca ggcgctgggt
      121 ttaccggcac aagacacgca acacattatt agtatgtcgc tgtttgcctc cggtgtggca
      181 tcgattattc aaattaaggc ctggggtccg gttggctccg ggctgttgtc tattcagggc
      241 accagcttca actttgttgc cccgctgatt atgggcggta ccacgctgaa aaccggtggt
      301 gctgatgttc ctaccatgat ggcggctttg ttcggcacgt tgatgctggc aagttgcacc
      361 gagatggtga tctcccgcgt tctgcatctg gcgcgccgca ttattacgcc gctggtttct
      421 ggcgttgtgg tgatgattat cggcctgtcg ctaattcagg tggggctgac ctccattggc
      481 ggcggttacg cagccatgag cgataacacc ttcggcgcac cgaaaaatct gctgctggca
      541 ggcgtggttt tagccttaat tatcctgctt aaccgtcaac gtaaccctta cttacgcgtg
      601 gcctcactgg taattgcgat ggcggccgga tatgcgctgg cgtggtttat gggcatgttg
      661 ccagaaagca acgaaccgat gacgcaagaa ctgattatgg tgccaacgcc gctctattac
      721 ggtcttggca ttgaatggag tctgctgctg ccgctgatgc tggtctttat gatcacttcg
      781 ctggaaacca ttggcgatat cacggcgacc tctgacgttt ccgaacagcc agtgtccggt
      841 ccgctgtaca tgaaacgcct gaaaggcggc gtgctggcaa acggcctgaa ctcgtttgtt
      901 tcggcggtgt ttaacacctt cccgaactcc tgcttcgggc aaaacaacgg agtgatccag
      961 ttgaccggtg ttgccagccg ctatgtcggt tttgtcgtcg cgctgatgtt gatcgtgctg
     1021 ggtctgttcc cggcagtgag cggttttgta caacacattc cagaaccggt tctgggcggc
     1081 gcaacgcttg taatgtttgg caccatcgcc gcctccggtg tgcgtatcgt ttctcgtgag
     1141 ccgctgaacc gtcgggcgat tctgattatc gcgctgtcgc tggcggttgg tctgggcgtg
     1201 tctcagcagc cgctgatttt gcagtttgcc cctgaatggc tgaaaaacct gctctcctcc
     1261 gggatcgccg cgggcggtat tactgccatc gtgctgaatc tgattttccc accagaaaaa
     1321 cagtaattca ctggcggcgg taagataatt accgccgcag tctcacctct ttaaccattc
     1381 ccgccttgag gattgcgcgt aaatcgtgca taactccctt atgtgcattt cacgggatgg
     1441 aagaccatga aatttattgg gaagctgctt ctctacattc tcatcgctct gttagtggcg
     1501 atcgctggcc tctattttct tctgcaaacc cgctggggag cagaacatat cagcgcatgg
     1561 gtttccgaga atagcgacta tcatctggcc ttcggggcga tggttcaccg tttttccgcg
     1621 ccatctcata tcgtgctgga gaatgtcacg tttggtcgtg atggccagcc cgcgaccctg
     1681 gtggccaaaa gtgtcgacat tgcgctaagt agtcggcaac tgaccgaacc acgccatgtc
     1741 gataccatcc tgctggaaaa cgggacgctg aatctcaccg accagaccgc gccgctaccg
     1801 ttcaaagccg atcgtctgca actgcgtgat atggcgttta atagcccgaa tagcgaatgg
     1861 aaactgagcg cgcagcgggt aaatggcggc gtggttccgt ggtcaccaga agccggtaaa
     1921 gtgctgggta cgaaggcgca gattcagttt agtgccggat cgctttcgct caatgatgtt
     1981 cctgccacca atgtactgat tgaaggcagt attgataacg atcgcgttac gctgactaac
     2041 ctgggtgccg acatcgcgcg cgggacatta accggaaacg cgcagcgtaa cgccgacggc
     2101 agctggcaag tggaaaacct gcgcatggcg gatatccgtc tacaaagcga aaaatcgcta
     2161 accgacttct ttgcgccatt acgctctgtc ccgtcgttgc agattggtcg cctggaagtg
     2221 atcgatgctc gtttgcaagg tccggactgg gcggtgaccg acctcgatct cagcttgcgc
     2281 aacatgacct tcagtaaaga tgactggcag acacaagaag gcaaactgtc gatgaacgct
     2341 agcgagttca tttatggttc gctgcattta tttgacccga ttataaacgc ggaattttct
     2401 ccgcagggcg tagcgctgcg ccagttcacc agccgctggg aagggggtat ggtcagaacg
     2461 tcagggaact ggctgcgtga cgggaaaacg ttgatccttg atgatgcggc aattgccgga
     2521 ctggaatata ccttgccgaa aaactggcaa cagttgtgga tggaaacgac acccggttgg
     2581 ttaaacagcc tgcaactgaa gagatttagc gccagccgca atctgatcat tgatatcgac
     2641 cctgacttcc cgtggcagct caccgcgctc gatggttacg gtgccaacct gacgctggtt
     2701 accgatcata aatggggcgt ctggagtggc tcggcgaatc tgaatgccgc cgccgcgacg
     2761 tttaatcgtg ttgatgtccg tcgtccgtcg ctggcgctaa ccgccaacag cagcacggtg
     2821 aatatcagcg aactgagtgc atttactgaa aaaggcattc tggaagccac cgccagtgtt
     2881 tcacaaacgc cacaacgtca gacacatatc agcctgaatg gacgcggtgt gccggtgaat
     2941 attttgcaac agtggggatg gcctgaatta ccgttgactg gcgacggcaa tattcagctt
     3001 accgccagtg gcgatattca ggccaatgtc ccgttgaaac ctacggttag cgggcaattg
     3061 catgccgtga acgccgcaaa gcagcaagtg actcaaacca tgaatgcggg cgtcgtttcc
     3121 agcggtgaag ttacatcggc agagctggtg cagtaaacat caatctcgcc gggtacgact
     3181 cagacgtacc cggcattctt caatagatta caacgtaatt gtcagcgcat tcccttgagg
     3241 cgtaaccacc agcccctgct cactttcagc ctgcgaaccg ccttgcagac catttacttt
     3301 cacaatattg cgcaagcaca gtgtccagtt cctcgcctcg cccgcaccag tcacagtaat
     3361 cgtgttacca gtacgtgttg cttttagagt aaagatcacc gaaccgtcag cagcgggcac
     3421 ttcacataca gcttcatgcc cgtcttgcag attaaagaga tggaatgccg tgccttcgtg
     3481 ccacgcgtaa tcgggacgtt gatcgttgtt gcccagcgcc aatagggtgt tatcacgcac
     3541 ataaacgggc agactcagga agccgtgctg ctgtttatgc cagcgactac cgtcgagctc
     3601 attgttgtgc cacaggtgtg tccagcgacc ttccggcaag tagaactgca cctcgcccgc
     3661 ctcagtgaac accggagcaa ccatcacgtt gtcgcctagc atgtattgac ggtcaaggta
     3721 atcacaagcc ggatcgtccg ggaactccat catcatggcc cgcatcatcg gcgtaccccg
     3781 cgctttcgca cgcgcagctt cacgatacag atacggcatc atgcggcatt tcagttgcgt
     3841 gaagaagcgc accacatcac aggactcatc atcgtaggcc cacggcacac gataagattt
     3901 gctgccatgt aaacgactat ggctggagag caaaccaaac gcgcaccagc gtttgtaaac
     3961 gtgtgccggc gcggtatttt caaagccgcc gatatcgtgg ctccagaagc caaaacctga
     4021 aaggccaata gacaaaccac cgcgcaggct ttccgccatt gattcgtagt tagcgtaaca
     4081 atcgccaccc cagtgtaccg ggaatttctg cgcaccgacg gaggccgagc gggcaaacaa
     4141 gacagcttct tcctcaccaa cggtgtcctt gagcacgttc cacaccagtt cgttgtagat
     4201 gtacgcataa tggttatgca ttttctgcgg atcggaaccg tcaaaccact gaacatcagt
     4261 tgggatacgt tcgccaaagt cggtcttaaa gcaatcaacg cccatcgcga ccagaccttt
     4321 cagtttgtcg gcgtaccatt tgcaggcatc cggattggta aagtcataaa tcgccaga
//
LOCUS       DAZPAC010000290         4346 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZPAC010000290 DAZPAC010000000
VERSION     DAZPAC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.292"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(101..796)
                     /locus_tag="ACHUPM_004094"
     CDS             complement(101..796)
                     /locus_tag="ACHUPM_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR0871729.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(789..2216)
                     /locus_tag="ACHUPM_004095"
     CDS             complement(789..2216)
                     /locus_tag="ACHUPM_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR0871730.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2227..2946)
                     /locus_tag="ACHUPM_004096"
     CDS             complement(2227..2946)
                     /locus_tag="ACHUPM_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR0871731.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="ACHUPM_004097"
     CDS             complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="ACHUPM_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFR0871732.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         1102 a         1193 c          931 g         1120 t
ORIGIN      
        1 agcataacca tgacggaacg gggttaagtc aggttgtcct taacgctatt ttcaacctcg
       61 tatgccttct tcaggtttat gttcagatct catatctctc tcagcaatcc tcaataatca
      121 gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca atatccgccg
      181 ttgagctggg accactgatg atgttaatac atgacggcat tcgttcaccg gcctgcgctt
      241 tctgatgcaa tttttctgcg agttgcgcta cacgcggcag gattgtgctt ttacgcagga
      301 taaaaagaga agattccggg agcaggctca atgaacgccc gcgctcgacg gcggaaaaaa
      361 gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc actttagcct
      421 gctctgcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg gcattgcatt
      481 cctgctgcaa acgttcacta atccccaatt cctccagcct cgtgtcaccg ctaatcacga
      541 ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc
      601 tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac gcgtcacagc
      661 gctgctgttg gttaagttgg gtaagccgct cgttagcata gttgttaagc ggcgcatctt
      721 ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta ttcaaaaatt
      781 cgctccgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca actacggaaa
      841 ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat cgcgccaaat
      901 ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcgcc ggccatcatc
      961 ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat cgcccgttgc
     1021 tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa aatcagtttt
     1081 gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga gcaggcgtag
     1141 ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc accaattggc
     1201 cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg acaagtattc
     1261 atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc cagcacctca
     1321 gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac gtgccccgct
     1381 tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc
     1441 gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg ctccattccc
     1501 atcacagcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc ttcattggtc
     1561 accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat acctatttca
     1621 gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc ttcaggcgtt
     1681 tccggcccct catagcccag acgttcgtgt agcactcgac ggatctgatg gcgatcttta
     1741 tgaattgccg ggaccacaac atgagatggc ggatcttgat ccagctggag aatatattca
     1801 cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
     1861 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
     1921 tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa accagacgtg accgccgttt
     1981 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
     2041 cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc gaccattttt
     2101 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
     2161 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
     2221 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
     2281 ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa ttaaatactc
     2341 agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg atatttcggc
     2401 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa
     2461 cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca ctcccagctt
     2521 acgggccagg ctacaagatg ggtgatacac agctctccct tgcaaactgg cacctacatc
     2581 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
     2641 ttcggcacgt gatgcccatt caggttcatc cgccagatac gtcgggtaac tttttacggc
     2701 ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca gtgcggcgat
     2761 cagatttttc atccctggaa tcgcttcttt gatataaccg ctattgatcg caggctgacc
     2821 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     2881 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     2941 attcacaact aatactccat tacttcatgc ccatttatgc ccgatggttc ccctccttcc
     3001 gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa cataccaccc
     3061 ctcatttgtg tgacaaatga taatctttca tccaaacttc aaatgcaata actaatttta
     3121 tttctttcgg catcgctcac attttatggg gaaatcgacg ttcatttttg gcataagaaa
     3181 tccatttaaa aaggctacat cccttatacc tattcagagc tacttattca ggggttattg
     3241 agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc aagtaatagt
     3301 cggggtttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac tgagctagag
     3361 tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg
     3421 ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt tattcagggt
     3481 gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc gcggacaaac
     3541 gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac cacaacaggg
     3601 agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt taatacagcc
     3661 agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg agacatgtgg
     3721 gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg gcatttccag catttgctgt
     3781 attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc tgtattaacc
     3841 tgtgcaatcc actcacgcac cgcgagggta aagaatgtag cgcagatctg gctacacagt
     3901 gcatccactc ccggcatcgc cgatctggat tcctgttgta aaaacgggat cgcctccctc
     3961 agccagttat attccacgct gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac
     4021 gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc cgcagacaat acaggtcgat
     4081 tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac cacatttccc
     4141 gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc ttgcgttaac
     4201 gcatgccaac gaataaccga taattccccg gcaccatgtt gaagctgcca gtcacttcct
     4261 aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa acggctaagg
     4321 gcatccatat cagactcctg agtggt
//
LOCUS       DAZPAC010000291         4330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZPAC010000291 DAZPAC010000000
VERSION     DAZPAC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.293"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            147..3239
                     /gene="ebgA"
                     /locus_tag="ACHUPM_004098"
                     /pseudo
     CDS             147..3239
                     /gene="ebgA"
                     /locus_tag="ACHUPM_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
     gene            3236..3685
                     /locus_tag="ACHUPM_004099"
     CDS             3236..3685
                     /locus_tag="ACHUPM_004099"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR0871733.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKMVLKVTIEDGYFHNK"
     gene            3748..>4330
                     /locus_tag="ACHUPM_004100"
     CDS             3748..>4330
                     /locus_tag="ACHUPM_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0871734.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
BASE COUNT         1044 a         1167 c         1089 g         1030 t
ORIGIN      
        1 acagtgctgc cgtttatttt cgtgatccag ttaaagtaaa tgcatttacc tgtcactttt
       61 tagtaaaaat tttactaaac tccccagcaa ttacacaaac taccatcacc atgaatggtt
      121 ccgatttctc tctaccggga ggccctatga atcgctggga aaacattcag ctcacccacg
      181 aaaaccgact tgcgccacgt gcgtactttt tttcatatga ttctgttgcg caagcgcgta
      241 cctttgcccg cgaaaccagc agcctgtttc tgcccttaag cggtcagtgg aatttccact
      301 tttttgacca cccgctgcaa gtgcctgaag ccttcacctc tgagttaatg gctgactggg
      361 ggcatattac cgtccccgcc atgtggcaaa tggaaggtca cggcaaactg caatataccg
      421 acgaaggttt tccgttcccc atcgatgtgc cgtttgttcc cagcgataac ccaaccggtg
      481 cctatcaacg tattttcacc ctcagcgacg gctggcaggg taaacagacg ctgattaaat
      541 ttgacggcgt cgaaacctat tttgaagtct acgttaacgg tcagtatgtg ggtttcagca
      601 agggcagtcg cctgaccgca gagtttgaca tcagcgcgat ggttaaaacc ggcgacaacc
      661 tgttgtgtgt gcgcgtgatg caatgggcgg actctaccta cgtggaagac caggatatgt
      721 ggtggtcagc ggggatcttc cgcgatgttt atctgatcgg aaaacaacta acacatatta
      781 acgatttcac cgtgcgtacc gactttgacg aagcctattg cgatgccacg ctttcctgcg
      841 aagtggtgct ggaaaatctc gccgcctccc ctgtcgtcac gacgctggaa tataccctgt
      901 ttgatggcga acgcgtggtg cacagcagcg ccattgatca tttggcaatt gaaaaactga
      961 ccagcgccag ctttgctttt actgtcgaac agccgcagca atggtcagca gaatcccctt
     1021 atctttacca tctggtcatg acgctgaaag acgccaacgg caacgttctg gaagtggtac
     1081 cacaacgcgt tggcttccgt gatatcaaag tgcgcgacgg tctgttctgg atcaataacc
     1141 gttatgtgat gctgcacggc gtcaaccgtc acgacaacga tcatcgcaaa ggccgcgccg
     1201 ttggaatgga tcgcgtcgag aaagatctcc agttgatgaa gcagcacaac atcaactccg
     1261 tgcgtaccgc tcactacccg aacgatccgc gtttttacga actgtgtgat atctacggcc
     1321 tgtttgtgat ggcggaaacc gacgtcgaat cgcacggctt tgctaatgtc ggcgatatca
     1381 gccgtattac cgacgatccg cagtgggaaa aagtctacgt cgagcgcatt gttcgccata
     1441 ttcacgcgca gaaaaaccat ccgtcgatca tcatctggtc gctgggcaat gaatccggct
     1501 atggctgtaa catccgcgcg atgtaccacg cagcgaaggc gctggatgac acgcgactgg
     1561 tgcattacga agaagatcgc gatgctgaag tggtcgatat tatttccacc atgtacaccc
     1621 gcgtgccgct gatgaatgag tttggtgaat acccgcatcc gaagccgcgc atcatctgtg
     1681 aatatgctca tgcgatgggg aacggaccgg gcgggctgac ggagtaccag aacgtcttct
     1741 ataagcacga ttgcattcag ggtcattatg tctgggagtg gtgcgaccac gggatccagg
     1801 cacaggacga ccacggcaat gtctggtata aattcggcgg cgactacggc gactatccca
     1861 acaactataa cttctgtctt gatggtttga tctattccga tcagacgccg ggaccgggcc
     1921 tgaaagagta caaacaggtt atcgcgccgg taaaaatcca cgcgcgggat ctgactcgcg
     1981 gcgagttgaa agtcgaaaat aaactgtggt ttaccacgct tgatgactac cccctgcacg
     2041 cagaggtgcg cgccgaaggt gaaacgctcg cgacgcagca gattaaactg cgcgacgttg
     2101 cgccgaacag cgaagccccc ttgcagatca cgctgccgca gctggacgcc cgcgaagcgt
     2161 tcctcaacat tacggtgacc aaagattccc gcacccgcta cagcgaagcc gggcattcta
     2221 tcgccactta tcagttcccg ctgaaggaaa acaccgcgca gccagtgcct ttcgcaccaa
     2281 ataatgcgcg tccgctgacg ctggaagacg atcgtttgag ctgcaccgtt cgcggctaca
     2341 acttcgcgat caccttctca aaaatgagtg gcaaaccgac atcctggcag gtaaatggcg
     2401 agtcgctgct gacccgcgag ccaaagatca acttcttcaa gccgatgatc gacaaccaca
     2461 agcaggagta cgaagggttg tggcagccga atcatttgca gatcatgcag gaacatctgc
     2521 gcgactttgc cgtagaacag agcgagggtg aagtgttgat catcagccgc acggttattg
     2581 ccccgccggt gtttgatttc ggaatgcgct gcacctacat ctggcgcatc gctgccgatg
     2641 gccaggttaa cgtggcgctt tccggcgagc gttacggcga ctatccgcac atcattccgt
     2701 gcatcggttt caccatggga attaacggcg aatacgatca ggtggcgtat tacggtcgtg
     2761 gaccgggcga aaactacgcc gacagccagc aggctaacat catcgatatc tggcgcagca
     2821 ccgtcgatgc catgttcgag aactatccct tcccgcagaa caacggtaac cgtcagcatg
     2881 tccgctggac ggcactgact aaccgccacg gtaacggtct gctggtggtt ccgcagcgcc
     2941 caattaactt cagcgcttgg cactatacct aggaaaacat ccacgctgcc cagcactgta
     3001 acgagctgca gcgcagtgat gacatcaccc tgaacctcga tcaccagctg cttggcctcg
     3061 gctccaactc ctggggcagc gaggtgctgg actcctggcg cgtctggttc cgtgacttca
     3121 gctacggctt tacgttgctg ccggtttctg gcggagaagc taccgcgcaa agcctggcgt
     3181 cgtatgagtt cggcgcaggg ttcttttcca cgaatttgca cagcgagaat aagcaatgag
     3241 gatcatcgat aacttagaac agttccgcca gatttacgcc tctggcaaga agtggcaacg
     3301 ctgcgttgaa gcgattgaaa atatcgacaa cattcagcct ggcgtcgccc actccatcgg
     3361 tgactcattg acttaccgcg tggagacaga ctccgcgacc gatgcgctat ttaccgggca
     3421 tcgacgctat tttgaagtgc attactacct gcaagggcag caaaaaattg aatatgcgcc
     3481 gaaagagaca ttacaggtag tggaatatta tcgtgatgaa actgaccgtg aatatttaaa
     3541 aggctgcgga gaaaccgttg aggtccacga agggcaaatc gttatttgcg atatccatga
     3601 agcgtatcgg tttatctgca ataacgcggt caaaaaaatg gttctcaaag tcaccatcga
     3661 agatggttat ttccataaca aataacaact acggcggcaa aaggagtttg ccgccaccgc
     3721 taccctactc attttcggag atgtgttatg tctgatacca aacgtaatac aatcggcaaa
     3781 ttcggcttgc tctcgctgac ttttgccgcc gtttacagct ttaacaacgt tatcaataat
     3841 aatattgatc ttggactggc ctcggcaccg atgtttttcc tcgcgacgat tttttatttt
     3901 attcccttct gtctgatcat cgcagaattt gtttcgttaa ataaaaactc agaagccggt
     3961 gtctacgcat gggtaaaaag ttcgctgggc ggacgttggg catttattac tgcctatacc
     4021 tactggttcg taaacctgtt ctttttcacc tcactgttgc cgcgcgttat tgcttatgct
     4081 tcgtatgcct tcctcggata cgaatatatt atgacgccgg ttgccaccac cattatcagt
     4141 atggtgctgt tcgccttctc cacctgggtt tccaccaacg gggcgaaaat gttggggcca
     4201 attacctccg tcacttcaac gctgatgctg ctgttaacgc tctcctacat tttactggca
     4261 ggtacggcgc tggttggcgg cgtacagcct gctgacgcca tcaccgttga cgcgatgatc
     4321 ccgaacttca
//
LOCUS       DAZPAC010000292         4289 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZPAC010000292 DAZPAC010000000
VERSION     DAZPAC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.294"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..572)
                     /locus_tag="ACHUPM_004101"
     CDS             complement(<1..572)
                     /locus_tag="ACHUPM_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0871735.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDP"
     gene            complement(783..2180)
                     /gene="ccp"
                     /locus_tag="ACHUPM_004102"
     CDS             complement(783..2180)
                     /gene="ccp"
                     /locus_tag="ACHUPM_004102"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR0871736.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2585..4234
                     /locus_tag="ACHUPM_004103"
     CDS             2585..4234
                     /locus_tag="ACHUPM_004103"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR0871737.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1101 a         1097 c         1012 g         1079 t
ORIGIN      
        1 gggtccataa acaactgacc ggggcgcata gggatctcac gcagctccac atcccagtag
       61 cgggcgaatt tatgccagca gatttgtacc ggaccgcaca ccaggtttgg tttatccgtt
      121 ggtttgcctg cagcttccat acgcttgcgc caacgccatt tcatcgccat cccgccgagc
      181 atacaggcct cggaagaacc aatggtgttg gtgccaacgg cctgaccatt tttcggcgca
      241 ggcgcatgcc acagatcggc aaccatattt acgcaacgca ggtcgatggc tgcggattgc
      301 ggatattctt ctttgtcgat ccagttttta ttgatcgaca aatccatcaa tttatggacg
      361 ttttcgtcgt cccaggtctg gcagaaagtg gccaggttct gacgagcgtt gccatcaaga
      421 tataattcat cattgataat ctggaatgcg acatcatcgc gcatttcgtg cagcggaaat
      481 cgttttgact ccgcgatagt agaaatggcc tttgcgccaa aacgtgaatc gagtagttct
      541 gagcggaaat ccgttaacag cttctggtcc atttcgaact ccttaaattt atttgaaggc
      601 aataaaaaag taggatttat ccgcaatgga agcaaggcat tacaggctta atttaaataa
      661 caaaatccta agcagaaaaa cgacttttta taatataaac atttaacatg ataatattaa
      721 aaacagactt tattatcgta ataaaaatcg cccaaacatt cctgttcggg cgatcaaatt
      781 aattattgtt tatcctgcat atacggcgtg tacaccccgt tcagactgtg caggaaagct
      841 acgatatcat ccacatcctc ctgcggcagc tctttgccta cctgatagcg cagcatcagt
      901 ttcaccgccc cgtccagcgt cggcacgtca ccgcgatgga agtacggtgc cgttaaagca
      961 acgttacgta aaccgggtac tttctgacgc aatttatcac gctcttcttt agtaacattc
     1021 atacgaccaa tatccgccgc cgtaatttcc ccaaagttaa agtctttttt cagccccaac
     1081 ggttcaaagg aacgtccacc gagaataata ccaccatgac aagttgcaca tttattatct
     1141 ttaaataatt gatagccttt tttctgttgc gccgtcagcg cattttcatc tccacgcaac
     1201 catttatcaa atggggaatc cggcgtaatt aatgttttct caaattcagc aatggcatca
     1261 gtaatatttt cgccactgaa accttgcgga tagacgccga ggaactgcgc tttaagctgc
     1321 ggatcttttt ccagcttagc aataatttcg tcccaagatt tcgacgccat ttcaatcggg
     1381 ttcaacggcg gtccaccagc ctgatcctgc aatgttgccg cacgaccatc ccagaactgc
     1441 tcaacgttaa atactgagtt aaataccgtc ggcgcgttaa tcggcccaac tgcgccacca
     1501 acaccaatcg atgtttttct gccatcgacg ccccccgcat tcaacgcatg gcaatgagcg
     1561 catgaaatgg tgctatcagc cgataaacgg ggatcgtgat acagcgcaaa gcccaacgcc
     1621 actttttgcg catcggtagg cagtttttgc gggatgggct gcaccggttc attgcgatgc
     1681 tccggagcag tatcattgct ggcgtaatat tccgcgcgct gttttgcaat ccaggccagt
     1741 atttccgccc gctcttcatc actcacctta cccgcccagt gaagcgcggt ataacgcgtt
     1801 ggcggcatag tttcatactg catcacccat tcaatcttat tcagatcgct ttgcgaaacg
     1861 ggtttatcag ccagcagtgc cgcacgcacg gcctcgaggt taaaagattt atatccaagc
     1921 ttaatgtcgt aatccatcaa ctgtttcgcg ccaggaatat aataataggc gggtaattct
     1981 gccgaaggcg tgtggcaata gtcgcatcct ttttcgcgga gaaaacctaa aaccttatta
     2041 ttttcactga cagcggatgc ctgaacatcg gcctgtttac tgcgtttatt atcgtggtac
     2101 cacacataac cagataaccc taaatagcaa atcgcgacgc cagccaggcc gatcgcggta
     2161 atacgtgaga ccattttcat tattcttccc tcacggtggt gagttattgt gacccagaaa
     2221 attttccaga atgctgcaaa cgtctctgcg agacaaagcc atcataaaaa acagtgagat
     2281 acgctttttg ataggaatca atcaattaca tgaatataaa ctatcaataa gatagcctgt
     2341 gcctattacc cagatcagga atggcatatc atgattgcgg cgagagcagg attgaatgtt
     2401 ctgaaaatga aaaacaacac gaaatccgta tttttataaa ttccatggat attagtgcgt
     2461 tatatttttt gacaggttaa taaacgtgat ccaccgcacg ctttgtcgcc caccaggcgg
     2521 agcgaatgac tacccttaaa gaaaagcccg ataattagcg acgaatttcg gaggttggat
     2581 ccttatgctc aatcagaaaa ttcaaaaccc taatccagac gaactgatga tcgaagtcga
     2641 tctctgctat gagctggacc cgtatgaatt aaaactggat gagatgatcg aggcagaacc
     2701 ggaacccgag atgattgaag ggctgcccgc ctctgatgcg ctgacgcctg ccgatcgcta
     2761 tctcgaactg ttcgagcatg ttcagtcggc gaaaattttc ccggacagta aaacctttcc
     2821 cgactgcgca cctaaaatgg acccgctgga tattttaatc cgctaccgta aagtgcgccg
     2881 tcatcgtgat tttgacttgc gcaagtttgt tgaaaatcac ttctggctgc cggaggtcta
     2941 ctccagcgag tatgtatcgg acccgcaaaa ttccctgaaa gagcatatcg accagctgtg
     3001 gccggtgcta acccgcgaac cacaggatca cattccgtgg tcttctctac tggcgctgcc
     3061 gcagtcatat attgtcccag gcggccgttt tagcgaaacc tactattggg actcctattt
     3121 caccatgctg gggctggcgg aaagtggtcg ggaagatttg ctgaaatgca tggccgataa
     3181 cttcgcctgg atgatcgaaa actatggtca catccccaac ggcaaccgca cctattattt
     3241 gagccgatcg caaccaccgg tttttgcgct gatggtggag ttgtttgaag aagatggtgt
     3301 acgcggtgcg cgccgctatc tcgaccacct taaaatggaa tatgccttct ggatggacgg
     3361 tgcagaatcg ttgatcccta atcaggccta tcgccatgtt gtgcggatgc cggacggatc
     3421 gctgctcaac cgttactggg acgatcgcga cacgccgcgt gacgaatcct ggcttgagga
     3481 cgttgaaacc gcgaaacatt ctggtcgccc gcccaacgag gtgtaccgcg atttacgcgc
     3541 gggggcggcc tccggttggg attactcttc ccgttggctg cgtgatactg gtcgtctggc
     3601 gagcattcgt accacccagt tcatccccat cgatctgaat gccttcctgt ttaaactgga
     3661 gagcgccatc gccaacatct cggcgctgaa aggcgagaaa gagacagaag cgctgttccg
     3721 ccagaaggcc agtgcccgtc gcgatgcggt aaaccgttac ctctgggatg atgaaaacgg
     3781 catctaccgc gattacgact ggcgacgcga acaactggcg ctgttttccg ctgccgccat
     3841 tgtgccgctc tatgtcggca tggcgaacca tgaacaggcc gatcgtctgg caaacgccgt
     3901 acgcagccgg ttactgacac ctggcgggat tctggcaagc gagtacgaaa ccggtgaaca
     3961 gtgggataaa cccaacggct gggcaccgtt acaatggatg gcaattcagg gatttaaaat
     4021 gtatggcgat gaccttctgg gtgatgaaat cgcgcgcagc tggctgaaaa cggtgaatca
     4081 gttctatctg gaacagcaca aaatgatcga gaaataccat attgccgatg gtgttccccg
     4141 cgaaggcggc ggtggcgagt atccgttgca ggatgggttt ggctggacta acggtgtggt
     4201 acgccgttta attggtttgt acggcgaacc ataatatttt tacagccagc cgctaacttc
     4261 ctgctggctg taaaattatc ctcttcagg
//
LOCUS       DAZPAC010000293         4272 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZPAC010000293 DAZPAC010000000
VERSION     DAZPAC010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.295"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            125..1807
                     /locus_tag="ACHUPM_004104"
     CDS             125..1807
                     /locus_tag="ACHUPM_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR0871738.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1859..3016
                     /locus_tag="ACHUPM_004105"
     CDS             1859..3016
                     /locus_tag="ACHUPM_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0871739.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2982..3104)
                     /locus_tag="ACHUPM_004106"
     CDS             complement(2982..3104)
                     /locus_tag="ACHUPM_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871740.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3319..>4272
                     /locus_tag="ACHUPM_004107"
     CDS             3319..>4272
                     /locus_tag="ACHUPM_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
                     /protein_id="HFR0871741.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAF"
BASE COUNT         1282 a          911 c          845 g         1234 t
ORIGIN      
        1 gactgttatt tccctttttt tagctaaatc tgctatctct ttatgtgact aacttcactt
       61 acatccactt atttctcttc gtaaaattac ttcggaatta agtacaataa gaagaggaac
      121 atttatgaag tctgcattaa agaaaagtgt cgtaagtacc tcgatatctt tgatactggc
      181 atctggtatg gctgcatttg ctgctcatgc ggcagatgat gtaaagctga aagcaaccaa
      241 aacaaacgtt gctttctcag attttacgcc gactgaatac agtaccaaag gaaagccaaa
      301 tattatcgta ctaaccatgg atgatcttgg ttatggacaa cttccttttg ataagggatc
      361 ttttgaccca aaaacaatgg aaaatcgtga agttgtcgat acctacaaaa tagggataga
      421 taaagccatt gaagctgcac aaaaatcaac gccgacgctc ctttcattaa tggatgaagg
      481 cgtacgtttt actaacggct atgtggcaca cggtgtttcc ggcccctccc gtgccgcaat
      541 aatgaccggt cgagctcccg cccgctttgg tgtctattcc aataccgatg ctcaggatgg
      601 tattccgcta acagaaactt tcttgcctga attattccag aatcatggtt attacactgc
      661 agcagtaggt aaatggcact tgtcaaaaat cagtaatgtg ccggtaccgg aagataaaca
      721 aacgcgtgac tatcatgaca acttcaccac attttctgcg gaagaatggc aacctcaaaa
      781 ccgtggcttt gattacttta tgggattcca cgctgcagga acggcatatt acaactcccc
      841 ttcactgttc aaaaatcgtg aacgtgtccc cgcaaaaggt tatatcagcg atcagttaac
      901 cgatgaggca attggcgttg ttgatcgtgc caaaacactt gaccagcctt ttatgcttta
      961 cctggcttat aatgctccgc acctaccaaa tgataatcct gcaccggatc aatatcagaa
     1021 gcaatttaat accggtagtc aaacagcaga taactactac gcttccgttt attctgttga
     1081 tcagggtgta aaacgcattc tcgaacaact gaagaaaaac ggacagtatg acaatacaat
     1141 tattctcttt acctccgata atggtgcggt tatcgatggt cctctgccgc tgaacggggc
     1201 gcaaaaaggc tataagagtc agacctatcc tggcggtact cacaccccaa tgtttatgtg
     1261 gtggaaagga aaacttcaac ccggtaatta tgacaagctg atttccgcaa tggatttcta
     1321 cccaacagct cttgatgcag ccgatatcag cattccaaaa gaccttaagc tggatggtgt
     1381 ttccttgctg ccctggttgc aagataagaa acaaggcgcg ccacataaaa atctgacctg
     1441 gataacctct tattctcact ggtttgatga ggaaaatatt ccattctggg ataattacca
     1501 caaatttgtt cgccatcagt cagacgatta cccgcataac cccaacactg aggacttaag
     1561 ccaattctct tatacggtga gaaataacga ttattcgctt gtctatacag tagaaaacaa
     1621 tcagttaggt ctctacaaac tgacggatct acagcaaaaa gataaccttg ccgccgccaa
     1681 tccgcaggtc gttaaagaga tgcaaggcgt ggtaagagag tttatcgaca gcagccagcc
     1741 accgcttagc gaggtaaatc aggagaagtt taacaatatc aagaaagcac taagcgaagc
     1801 gaaataacta aaccttcatg cggcggattt ttccgccgcc ttattgagcg agatagcgat
     1861 gcacgttaca gccaagccct ccagttttca atgtaatctc aaatgtgatt actgttttta
     1921 ccttgaaaaa gagtcgcagt ttactcatga aaaatggatg gatgacagca ctctgaaaga
     1981 gttcatcaaa caatatatcg aagcgtctgg caatcaggtc tattttacct ggcaaggcgg
     2041 tgaacccact ctggctggcc tggatttttt ccgtaaagtt attcactatc aacaacgcta
     2101 tgcaggccaa aaacgtattt ttaatgcatt acaaacgaat ggcattttat tgaataatga
     2161 atggtgtgcc tttctcaaag aacatgaatt tctggttggt atctcgatcg atggccccct
     2221 ggagttacat gaccgttaca gacgcagtaa ttcaggtaac ggtacttttg caaaagtgat
     2281 agcagccatc gagcgtctga aatcatatca agtagagttt aatacgttaa ccgtcattaa
     2341 taacgttaat gtccattacc ctcttgaggt ttatcatttt ttaaaatcta tcggcagtaa
     2401 acatatgcaa tttatcgaat tgctagaaac cgggacgccg aatattgatt tcagtggtca
     2461 tagtgagaac acattccgta tcattgattt ttctgtgcct cccacggctt atggcaagtt
     2521 tatgtcaacc atttttatgc aatgggttaa aaacgatgtg ggtgaaattt tcattcgtca
     2581 gtttgaaagc tttgtcagcc gttttttggg gaatgggcat acaagttgta ttttccagga
     2641 gtcctgcaag gataatctgg ttgttgaaag taatggagac atttacgaat gcgaccattt
     2701 tgtctatcca cagtacaaaa ttggaaacat taataaatct gaactcaaaa cgatgaacag
     2761 tgtacaactg acagcgcaaa aaaaacggat ttcagcgaaa tgtcagcaat gtgcatataa
     2821 acctatctgc aatggcggtt gtcctaagca tcgtattact aaagtaaaca atgagactgt
     2881 ttcttatttt tgcgaaggtt ataaaatcct tttttcaacc atggtacctt atatgaacgc
     2941 catggttgag ttagctaaga acagagtacc gctttaccac attatggatg ttgcaaaaca
     3001 aatggagaat aattaataac gctctctttt aactccattc ttagcctcct tcttatgacc
     3061 ttaacaaggc acccgacata aattcctgtc gggtgtttcg tcatcatcat cttcccttgc
     3121 gaaaatcctg gcttacgtgg tatgcgtcag ggcattagca atacccttga ttatcaatag
     3181 cataaccata accataacca taaccactga aaattaaaaa tttctatata tttcatattg
     3241 ttaaatgata cttaaataaa tttcatcagt aaatcattgc catagataat cattggatgg
     3301 cacaatcaat tttcgctaat gataaccatt cccattacgt tttatatgtt gatagcaaag
     3361 tatctctgtc tgctgaagcc cttttggctg cgtaagaata ataaaacttc agtgttgtta
     3421 atcattatta tcctcgcaat gatccttggc gtggttaaaa ttcaggtctg gttaaacgac
     3481 tggaataatg attttttcaa tgcgctaagc cagaaagaaa ccgataagct ttggcagctt
     3541 gttctttggt tccctgccct gctggggatc tttgtattaa tatccgtaaa caaaacatgg
     3601 ttaattaaac tactgactat tcgctggcgc gaatggttaa cagattacta ccttaatcgc
     3661 tggtttgcag ataaaaacta ctacttcacg cagatctacg gcgagcataa aaatacagat
     3721 aaccctgacc aacgtatcgc tgaagacatt ctcttactga tcagcaaaac gctcagtctt
     3781 tcatttggct tcatccagtc acttagcatg ctgatcacct ttaccgttat tctttggcaa
     3841 agcgcgggta cgctctcatt cactgttggt ggaacagaat ggaatatcca ggggtatatg
     3901 gtctataccg tcgtgctcat tgtgatcggt ggaactttat ttacccataa ggtgggtaaa
     3961 cgtattcgtc cgcttaatgt ggaaaaacaa cgtagcgaag cgacatttcg aactaatctt
     4021 gtgcagcata ataagcagac agaactgatt gcgctaagca atgctgagtc tctacaacgc
     4081 caggagttgc gcgacaattt tcataccatt aaagagaact ggcatcgttt aatgaatcgt
     4141 cagcggtggc ttgattactg gcaaaatatt tattcgcgct cgcttagcgt tcttccctac
     4201 tttctgttat tgccgcagtt tattagcggg cagatcaatc tgggcggact gatgaaatcg
     4261 cgccaggcat tt
//
LOCUS       DAZPAC010000294         4227 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZPAC010000294 DAZPAC010000000
VERSION     DAZPAC010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.296"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..195
                     /locus_tag="ACHUPM_004108"
     CDS             <1..195
                     /locus_tag="ACHUPM_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR0871742.1"
                     /translation="EYYGKFWSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTL
                     VRKEYPKLFVHWYLLRASNE"
     gene            278..457
                     /locus_tag="ACHUPM_004109"
                     /pseudo
     CDS             278..457
                     /locus_tag="ACHUPM_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(484..969)
                     /locus_tag="ACHUPM_004110"
     CDS             complement(484..969)
                     /locus_tag="ACHUPM_004110"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR0871743.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAADLRQ
                     K"
     gene            complement(1225..2205)
                     /locus_tag="ACHUPM_004111"
     CDS             complement(1225..2205)
                     /locus_tag="ACHUPM_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR0871744.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIHLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(2205..3404)
                     /locus_tag="ACHUPM_004112"
     CDS             complement(2205..3404)
                     /locus_tag="ACHUPM_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0871745.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(3578..3712)
                     /locus_tag="ACHUPM_004113"
                     /pseudo
     CDS             complement(3578..3712)
                     /locus_tag="ACHUPM_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3726..4079)
                     /locus_tag="ACHUPM_004114"
                     /pseudo
     CDS             complement(3726..4079)
                     /locus_tag="ACHUPM_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4146..>4227)
                     /locus_tag="ACHUPM_004115"
     CDS             complement(4146..>4227)
                     /locus_tag="ACHUPM_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871746.1"
                     /translation="FVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT         1152 a          981 c          921 g         1173 t
ORIGIN      
        1 gagtactacg gaaagttctg gtccagaaat ttcaactatc gactgtggag tgcaatgcag
       61 tcacgtctgc tcaagtggat gcagtctaaa tacagacttt cgaaccggaa ggctcagcga
      121 aagctgacgc tggtaaggaa ggagtatccg aagctatttg ttcactggta tttactacgt
      181 gcatcaaatg agtgatcaag agccgtatga cgcgagagtg tcacgtacgg ttccgtgagc
      241 agccggggag tgaaattctc tccggctgac tcgactcgcg ggttccgaca gcgatgacga
      301 acatgcggcg gtgttgtact cgctgatcgg cacatgccgt ctgaacaatg tggaaccaga
      361 aaaatggctg cgttacgtca ttgagcatat ccaggactgg ctggcaaacc gggtacgtga
      421 tctgttgccc tggaaagttg atctgagctc tcagtaaata tcaatacggt tctgacgagc
      481 cgcttacttt tggcgtaaat ctgctgcgcc tttactgaac acaatgccgt attcagcaag
      541 aagtccacga atgtgattta tcagttgtgt cctttccatt gaaaccaacc gacgcatatt
      601 caaaaaagtc tggacgtcct gttgctccag agtttttatt ggtacgggtc gtatagttcc
      661 atgttggcag gcctcagcta ttgcctgtgc gtcgttataa tcatttttct ggcaccgctg
      721 ataagcttta acatgttgtg ccggaagcaa tattacatca tggtcgagtt tcctgatttc
      781 ccgggcccag taatgagatg ccccgccagc ctccatcgca acagtcattt taggctgttg
      841 caccagccag ttaagcagtt gattgcgaga aagtttggtc tttcctgcgg gtttgccatg
      901 agcattgata ctgaaaagat aaaagttcgt tttagccaga tcaatgccgg tgattttctg
      961 ttcagacatg gtattccgct tacttttgtg gttaaagttt atccatcatg gcgcaattga
     1021 aggctgccgt gaaagagggg gaagactatc tcatcggtta ctgttaaacg gctaagagaa
     1081 actatctttt tcaagcaaag aagatacggt gaaacaggcg atttcaccgt gaaatactgt
     1141 tcaaaaaaaa cgctaataaa agcaaaattt cttttaaaac agccaattaa aaactggtga
     1201 gttgctggcg aaaaacttga attttcacag cgaattcaga ttatctttta gtacaagagc
     1261 aatcatacga tctaacttat cctgaacttc caacggtaat ctgccgaatt catatgagaa
     1321 cgtacgtccc tttactcgtt ttcgagcaaa tttatcttta ctatcaaatt tccataacaa
     1381 agttacaacc gctttatcct ttactcctga atcaagcaat gaagacattt ccttggttat
     1441 aagccgcata atactgtttt taacctcgtc ttcagaaaga ttgtcatcag cagtaataag
     1501 ctcaatactc ggcgagatat tcttcacaac ttcatcaata gatatatcac ccagcctcag
     1561 tcgttcactt aattcagcca gttgcctgta atcggcgtat gtcagctcag actgaacagg
     1621 aaaaagagac acaaaatctt taggtacgga cgctgcttgg agggcacgag tcaccttagc
     1681 tgccgacaat ccctctcttt ccgcaatttg cttctggttc ataccggcct ctttgagacg
     1741 tactaaacgc aacccaattt cacgaatatt atgttcaagt gacgtctgca aatctttcgc
     1801 cagatgctga gcttcagaaa cagtaagctc atcatccgtt acaagtacac gtaatccaac
     1861 cttgcatagc aaagctgctg cccgacgacg ggaaccatcc aaaatctcaa taagatcgcc
     1921 agtccgaatc ccaatacatg gatagaattg ttgtaaatgt atagttctgg tgatgtcttt
     1981 tagtgagtcc ggcgtcaggg ctgtctgttc gcggccattc acctcttcaa ccacaaaagt
     2041 ttgagtgtcc actttgtcat gatcaacctt gatctcagta aattttgcct ttcggccagt
     2101 attgagggta aatacatgaa ctggagccga tatttcttca gtattattca ggatatttgt
     2161 attcagcgta cggccaatag tcggtcggtg ctttctgttt tccatcagta gttggccctt
     2221 ataaattcaa tacgatcaaa aactgcttta gcgaaatctt ctgctgccat acgtgcattt
     2281 ttcaaagctt caccactgcc aacataagta acaggattcg cagatatcac ggtatcaaac
     2341 gactcgccgc ttctctcaaa accatcaaga cgtggcattg aaacatcaag catgtcacca
     2401 ccgaaaatct cttttgtcag gctatggcag tatttatgat ctgacttatt cgccagtttc
     2461 gacatgaagc caatatttgc ctgcaaacgg catgaacatc catcctgttc aataatctgt
     2521 acaagttcag gtaaacgagc caaatattta agcgtggaat ggaaatcaac ttgtgctggc
     2581 ggcaccggtg taaacatgat atctgcggca gcaatggcat ttttcaaaaa tgcgtccaga
     2641 tgcgggcctg tatcaataag gatgaagtcg aaatcatgct tcagcttatc aataatattc
     2701 tcacgcaata tcgcgtgttt gttttgtcct aatagatgct cctcgcacag agtatcccag
     2761 tttgatgcaa taaaggcgtc gtcaatagaa gccggcataa cataaacgcc agggataact
     2821 gacggaacga taaaatcttc cagtagttcc tcacgactca cgttctgcaa catagcctgc
     2881 ggtgcggttg tatcaaccag ccccaccgca tgcaagtagt tcagaaacat agtggctgat
     2941 gactggggat ctaaatcaag gagcagaata cgtaaatctt ctgacagcag gtgaggatga
     3001 gcacgcaggg cgtgagctac ggaaacacta ctcacggttt tagacacacc gccttttaga
     3061 ctaccgatga atatggaata tgcctcacta taacgatcgc gatatttagg aatgccacga
     3121 tgtgcataga tatcgataat gttctggatg gttaatgcat attttgtcgc ggttcctgct
     3181 gggcgttttt caaactgata gccctgagcc tccatctcac cgactgcacc atccacgctt
     3241 gcgcgcgtta attttggaag cttagctaca gcagactttg aatagacttg ataaaaaaca
     3301 tcagcctgaa actcctgctt ttgctcctga atctgttttg ttagggcgag caacatttta
     3361 tctgcacgct gcgcaacctt acttaactgc tcaaaactag tcattccccc tccgtgctat
     3421 gtaggatttt aacttattac aaaaaaaact gcattagcag ggtaatgcat gcaagtacat
     3481 attgtacatg cttatattaa aaatgcgcgc attgctgatt tattaatcat cggaatgaat
     3541 attactgtaa gatagtatgc ctttccggga gaaagacgct gatttccccc gaataatcca
     3601 gttcaccacc acactccgga caacagcttt cttcaggctc cagatggtgt atttcgcggg
     3661 aaacatgttc cggtaacgga cggcgatgac gtgactgtcg caacaggcgg gggtagtgct
     3721 tccaatcagt agaacatgtg cttttcaatg aaggagccga tgactttttc gtggatttcc
     3781 acggaacgcg agaagcagat tgttttacgt gccagccgct tgatgcgggt gcggagggtc
     3841 cggttgttac gttcaatgcg ttgcgtgaag attttgccgg tcaggtgttt ctccttcggc
     3901 acttctctgg cgtagctgcc ccagtcatcg ctggtcacca tgccaccatg ccgatacaga
     3961 acggagtcaa cagggctaac agttcccgac aggtctcatc attccgtggc ccgaaagtcg
     4021 aaagtgtaag ccaacacacc gccagtttta gtgttgtacg cataccagag ccagtgttgt
     4081 gtcaacgacg gatgaaaagt gatccactta tatctccacc aacggcccaa tattgatcca
     4141 ccgttttact caggattagc ttctgctata accccggcct ttcgtttctg tctgagtcga
     4201 tagctttctc ctttgatttg aacgaaa
//
LOCUS       DAZPAC010000295         4219 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZPAC010000295 DAZPAC010000000
VERSION     DAZPAC010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.297"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(49..3099)
                     /gene="fdnG"
                     /locus_tag="ACHUPM_004116"
     CDS             complement(49..3099)
                     /gene="fdnG"
                     /locus_tag="ACHUPM_004116"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2512..2514),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0871747.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            3293..4126
                     /gene="fdhD"
                     /locus_tag="ACHUPM_004117"
     CDS             3293..4126
                     /gene="fdhD"
                     /locus_tag="ACHUPM_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR0871748.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT          905 a         1099 c         1161 g         1054 t
ORIGIN      
        1 ggaacgacga atgatatctt gcgattgata agccatatgt cgtctccgtt acaccttttc
       61 cacattcaca aggaaggact taaactccgg cgtctgcgtg ttcgcatcac caacgaatgg
      121 cgttaacgta ttggcaataa agcctttttt agctacgcct tcatagcccc agtgaatcgg
      181 aataccgatg gtatcgatat ctttgccgtt cgctttcagc gtgcgaatac gtttggtcac
      241 caccgctttg gctttgatat agccacggtt ggaggagact ttcacggtat cgccctgggc
      301 aatgccaagt ttattcgcca gcgactcccc gatttccaca aactgctctg gttgcaaaat
      361 cgcgttcaac agcgcgtgtt tggtccagta gtggaagtgc tcggtcagac gataggtggt
      421 tccgacatac gggaacttat cggctttacc caatgcttcg gcgtcgtctt taaagattcg
      481 cgcagccggg ttcgagataa cgtttggatg cagcgggtta gttcccagcg gcgtttcaaa
      541 cggctcgtag tgttccggga acggaccttc cgccatctta tcgagggcaa acagacgccc
      601 catgccttcc tgctgcatga taaacggccc gacgccgctg cccggaggcg ctgcgctgta
      661 gtccggaata tcccagccgg tccacttagt gccgtcccat ttcagcaact gacgcttcgg
      721 atcccacggg ttaccctgcg gatctgcgga ggcgcggtta tacagaatgc ggcggttaag
      781 cggccatgcc catgcccagc ccagcgtgtt accgaggcca gacggatcgg cgttatcacg
      841 acgcgccatc tggttgcctt ccggcgtcca gctaccggcg aaaatccagc agccacagga
      901 ggttgtaccg tcatcgcgca attgggcgaa cgagctaagt tgttggcctt tcttgacgat
      961 aaccgcaccg gttgccggat cggtaatatc ggccagcgcc ttaccgttgc tctccatcgc
     1021 cacttcttcc gacttcggct catgcggaat ggcgtagttc caggtcatgt tcagcacctg
     1081 atccgggttt gcgccaccct gttcggcata catcttgcgc aagcgcagga agataccgga
     1141 gaggatctcg ccatcagtca gcgcaatccc tggggcgtcc gcacctttcc agtgccactg
     1201 caaccagcga cctgagttaa cgattgaacc gttctcttct gcgaagcagg tcgatggcag
     1261 acggaacact tcggtctgga tcttcgacga gtcaacttcg ttcagctcac cgtggttctg
     1321 ccagaagtta gaggtttcag tgttcagcgg gtcgatggtg acgaggaact tcagtttcga
     1381 cagacaaccg atcactttgt ttttgttcgg gaatgaggca acagggttaa agccctggca
     1441 gatatagcca ttgaccttgc cctctttcat catctcgaag tattgcagga cgtcgtagcc
     1501 tttatcccac ttcggcaacc agtcaaagcc ccagctattt tccgccgtcg ctttatcacc
     1561 aaagaaggcc ttcatcatag agacgaagaa tttcgggtag ttgccccagt agtttacctg
     1621 gccttccagc agcggttttg gcgtgttggc ggtaaggtag gtttgcagat cggtctgctt
     1681 ctcgcttggc agcgtcatgt aacctggcag gctctgcgac agcagcccca ggtccgtcag
     1741 cccctgaata ttggagtgac cgcgcagggc gttaacgccg ccgcctgcca tccccatatt
     1801 gccgagcagc agctggatca tcgccatcgt acgaatgttt tgcgcaccaa cggagtgttg
     1861 cgtccagccg agggcataca ggaacgaggc agttttatcg tgagcactgg tttctgcgat
     1921 gtattcgcag actttcagga acgcgtcttt tggcgtacca cagatgtttt caaccacatc
     1981 tggcgtgtag cgggaaacgt gctgtttcag caagttccac acgcagcgcg gatgttgcag
     2041 cgtggtatcg cgtttggcga agccgttttc gtccagttcg taagtccagg aggatttatc
     2101 gtacttgcgt ttttccgcgt catagccggt gaacaggcca tcttcaaagc cgtaatcctc
     2161 acgcacgatc aggctggcgt tggtataggc ttcggtgtat tcgcggttga atttttcatt
     2221 gttcagcagg tacagcaata cgcctgacag gaaagcaatg tcagtaccgg aacgaatagg
     2281 ggcatagtag tcagccaccg ccgccgtacg cgtaaagcga ggatcgatca caatcagctt
     2341 cgcgccattg tgaattttgg cttccatcgc ccagcggaac ccgaccgggt gagcttcagc
     2401 ggcgttaccg cccatcacca cgacgaggtt ggcgttcttg atgtcgaccc agtggttggt
     2461 catcgcaccg cgaccaaatg ttggagcaag acttgctacc gttggtccgt gtcagacacg
     2521 cgcctggttg tcgaccgcga gcatacccag cgcgcgggag aatttttgcg ttaaatagcc
     2581 ggtttcgtta ctcgacgcgg aagcacacag catcccggtg gagagccagc ggttaacagt
     2641 cacgccttcg gcgttttgcg caatgtagtt agcatcgcgg tcttctttca tcagtttggc
     2701 gatgcgatca aacgcctctt cccaactgat ttgctgccat ttatcagaac ctggcgcacg
     2761 gtattccgga aacttcagac ggctttcgga gtggatgaaa tccaccaggc cagcgccttt
     2821 cggacaaagt gcaccgcggt tgaccgggtg atccggatcg ccttcgatat ggaagataga
     2881 tgctttggcg ttttttgctc cgtcaccgag gctgtacatc aacagcccac agcctacgga
     2941 acaataggtg caggtattac gggtttcgcg ggtgcgcagc agtttatact gccgggtttc
     3001 cgcgagtgct acgctgggtg caaaacccag tgccgctgcc gtggtgcctg ccataccgcc
     3061 agcgcagatc ttaaagaact gccttctgct gacctgcatg gattgctcct tgtttcgaca
     3121 ttgtcacgtc ccatttacat tcgcttgctg cgtgtgcagg gagtgggagt tatttttctt
     3181 tgcggaaggg gccgcaaagg tccagaattg gctcaatttc cctccatcca ggaagggttt
     3241 gtaacagaat accataatgt tggtgtgtgt gttcttatct ggttaagaga aagtgaaaaa
     3301 aacacagcga aaagaaatcg aaaatgtgac aaatatcaca ggtgttcgtc aaattgagtt
     3361 atggcggcgt gatgatttac aacacccacg gttagatgag gtcgcggaag aagttcccgt
     3421 tgcgctggtc tacaacggca tttcgcatgt ggtgatgatg gcgtcgccca aagaccttga
     3481 gtactttgcg ctcggttttt cgctttccga ggggataata gaaagtccgc gcgatatctt
     3541 cggcatggat gtcgttcctt cctgtaatgg tcttgaagta caaattgagc tttccagccg
     3601 ccgctttatg gggttgaagg agcgccgccg ggcgctggcg ggacgtacgg gctgtggcgt
     3661 atgcggcgtg gagcaactta atgacatcgg aaaaccggtg cagccgctac cgttcaccca
     3721 gacgtttgat ctcaacaaac tggatgatgc attacgtcat ctcaacgatt ttcagccagt
     3781 ggggcaactg actggttgta ctcacgccgc tgcctggatg ttgccatctg gcgaactggt
     3841 cggcgggcat gaagacgtgg gtcgccatgt ggcgctggac aaactgttag gccgccggtc
     3901 acaagaaggg gaaagctggc agcaaggtgc ggtactggtt tccagtcgtg ccagttatga
     3961 aatggtgcaa aagtcggcga tgtgcggcgt agagattttg tttgcggtgt ctgccgcgac
     4021 cacgcttgct gtagaagtgg ctgagcgctg taatctgaca ctggtaggtt tttgtaaacc
     4081 gggtcgggca acggtttata cccatccgca gcgtttgagc aattaattaa aataagtcct
     4141 gcgatatata ttaaattgca ggacttattc atttcgtgaa ttttattatt ttatttataa
     4201 ataaaacgcg tgtagtgac
//
LOCUS       DAZPAC010000296         4192 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZPAC010000296 DAZPAC010000000
VERSION     DAZPAC010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.298"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUPM_004118"
     CDS             complement(<1..215)
                     /locus_tag="ACHUPM_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0871749.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="ACHUPM_004119"
     tRNA            complement(330..405)
                     /locus_tag="ACHUPM_004119"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="ACHUPM_004120"
     tRNA            complement(412..486)
                     /locus_tag="ACHUPM_004120"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="ACHUPM_004121"
     tRNA            complement(603..687)
                     /locus_tag="ACHUPM_004121"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="ACHUPM_004122"
     tRNA            complement(696..771)
                     /locus_tag="ACHUPM_004122"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="ACHUPM_004123"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="ACHUPM_004123"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR0871750.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHUPM_004124"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHUPM_004124"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR0871751.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHUPM_004125"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHUPM_004125"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR0871752.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1040 c          936 g         1136 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatct tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 acttcccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAZPAC010000297         4186 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZPAC010000297 DAZPAC010000000
VERSION     DAZPAC010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.299"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..324
                     /locus_tag="ACHUPM_004126"
     CDS             <1..324
                     /locus_tag="ACHUPM_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0871753.1"
                     /translation="FYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYS
                     AGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKEN
                     VAPGG"
     gene            551..1816
                     /locus_tag="ACHUPM_004127"
     CDS             551..1816
                     /locus_tag="ACHUPM_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0871754.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1828..2694
                     /gene="ydiB"
                     /locus_tag="ACHUPM_004128"
     CDS             1828..2694
                     /gene="ydiB"
                     /locus_tag="ACHUPM_004128"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR0871755.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2725..3483
                     /gene="aroD"
                     /locus_tag="ACHUPM_004129"
     CDS             2725..3483
                     /gene="aroD"
                     /locus_tag="ACHUPM_004129"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR0871756.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            3627..>4186
                     /locus_tag="ACHUPM_004130"
     CDS             3627..>4186
                     /locus_tag="ACHUPM_004130"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFR0871757.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIR"
BASE COUNT         1092 a          938 c         1005 g         1151 t
ORIGIN      
        1 ttttatgtgg tgataatatt tgcttttgtc attggtttta cctctgctgg aggtgttgtg
       61 caaattggcc tgacgttaat ggctgaacgt ttcccttacg ctaaaggtaa agctacaggg
      121 atctattaca gtgcgggcag tattgcgacc tttactattc cgttgattac ggctcatctg
      181 tcccaaagaa gtattgccga tattatgtgg ttcgataccg ccatcgctgc catcggtttt
      241 ttactggcac tgtttatcgg cttacgcagc cgcaaaaaaa cgcggcatca ctcgctaaag
      301 gaaaatgtcg ctccgggtgg gtaatgcaat attcttttca ggtcatgcaa gatcttacgg
      361 ataaataact ctttctgcgc taactaagga aaatcgcgat caaaaacaaa ctatgacatg
      421 caatattcct ggaaacataa actttatgcc atgtacccag ggaaaatcat cttcagtata
      481 gtaattatgt aaaccgtcgg agaacaatac gtacggtaac gaaattatct ttcagcaagg
      541 agctgtgaaa atgtctcaaa ataaggcttt cagcacgcca tttatcctgg ctgttctttg
      601 tatttacttc agctatttcc tgcacggcat tagtgttatt acgcttgccc aaaatatgac
      661 atctctggca gaaaagtttt ccactgacaa cgcgggcatt gcctatttaa tttccggtat
      721 cggtttgggg cgattgatca gtattttatt cttcggtgtg atctccgata agtttggtcg
      781 tcgggcggtg atattaatgg cagtaataat gtatctgcta ttcttctttg gtattcccgc
      841 ttgcccgaat ttaactctcg cctacggtct ggcagtgtgc gtaggtatcg ctaactcagc
      901 gctggatacg ggtggctacc ccgcgctcat ggaatgcttt ccgaaagcct ctggttcggc
      961 ggtcatactg gttaaagcga tggtgtcatt tgggcaaatg ttctacccaa tgctggtgag
     1021 ctatatgctg ctcaataata tctggtacgg ctatgggctg attattccgg gtattctgtt
     1081 tgtactgatc acgctgatgc tgttgaaaag caaattcccc agccagttgg tggacgccag
     1141 cgtagctaat gaattaccgc aaatgaacag caaaccgtta gtctggctgg aaggtgtttc
     1201 atcggtactg ttcggtgtag ccgcattctc gaccttttat gtgattgtgg tgtggatgcc
     1261 caaatatgcg atggcttttg ctggtatgtc agaagctgag gcattaaaaa ccatctctta
     1321 ttacagtatg ggctcgttgg tctgtgtctt tatttttgcc gcactactga aaaaaatggt
     1381 ccggcccatc tgggctaatg tatttaactc tgcgctggca acaataacag ctgccattat
     1441 ctacctgtac ccttctccac tggtgtgtaa tgccggcgcc tttgttatcg gtttctcagc
     1501 agctggcggc attttacagc tcggtgtttc ggtcatgtca gagtttttcc ccaaaagcaa
     1561 agccaaagtc accagtattt atatgatgat gggtggactg gctaactttg ttattccgct
     1621 gattaccggt tatctgtcga acatcggcct gcaatatatc attgttctcg attttacttt
     1681 cgcgctgctg gccctgatta ccgcaattat tgtttttagc cgctattacc gcgtttttat
     1741 tattcctgag aatgatgtgc ggtttggcga acgcaaattt agcacccggt taaacacaat
     1801 taagcataga ggttaaagga gttaattatg gatgttaccg caaaatacga attgattggg
     1861 ttgatggctt atcctatccg ccacagttta tcgcctgaaa tgcagaataa agccttagaa
     1921 aaagcgggat tgccatttac ctatatggcc ttcgaagtgg ataacgatag ctttcccgga
     1981 gcaattgaag gattaaaagc cctcaaaatg cgcggaactg gtgtgtcgat gccgaacaaa
     2041 caactggcgt gtgaatatgt tgatgaatta acgccagcag ccaaactggt gggtgccatc
     2101 aacaccatcg ttaatgatga tggctatctg cgtggctata acaccgacgg cacgggccat
     2161 attcgcgcca ttaaagagag cggttttgat atcaaaggca aaacgatggt gctgttaggg
     2221 gccggaggtg cctcaacagc gattggcgcg cagggggcaa ttgaaggttt aaaagaaatt
     2281 aaactcttta accgtcggga tgagttcttc gataaagccc tcgccttcgc gcagcgggtt
     2341 aacgaaaaca ccgattgtgt cgtcacggtt accgatctcg ccgatcagca agcctttgct
     2401 gaagtcctgg cttccgccga cattttaacc aatggcacaa aagtaggtat gaaccccctt
     2461 gagaatgaat cattggttaa tgatatcagt ctgttacatc cgggactgct ggtcactgaa
     2521 tgcgtgtata acccgcatat gacgaagtta ttgcagcagg cacaacaagc aggttgcaaa
     2581 acgattgatg gatacggcat gttgttgtgg caaggggctg aacagttcac gttatggact
     2641 ggcaaagatt tccctctgga atatgttaaa caggtcatgg ggttcggtgc ctgacaggct
     2701 gaccgcgtgc agaaagggta aaaaatgaaa accgtaactg taaaagatct cgtcattggt
     2761 gcgggcgcac ctaaaatcat cgtctcgctg atggcgaaag atatcgcccg cgtgaaatcc
     2821 gaagctctcg cctatcgtga agcggacttt gatattctgg aatggcgtgt ggaccacttt
     2881 gccgacctct ccaatgtgga gtctgtcatg gcggcggcaa aaattctccg tgaaaccatg
     2941 ccagaaaaac cgctgctgtt taccttccgc agtgccaaag aaggcggcga gcaggcgatt
     3001 tccaccgagg cttatattgc tctcaatcgt gcagccatcg acagcggcct ggttgatatg
     3061 atcgatctgg agttatttac cggcgatgat caggttaaag aaaccgtcgc ctacgcccac
     3121 gcgcatgatg tgaaagttgt catgtccaac catgacttcc ataaaacgcc ggaagccgaa
     3181 gaaatcattg cccgtctgcg caaaatgcag tccttcgacg ccgatattcc taagattgcg
     3241 ctgatgccgc aaagtaccag cgatgtgctg acgttgctta ccgcgaccct ggagatgcag
     3301 gagcagtatg ccgatcgtcc aatcatcacg atgtcgatgg caaaaactgg cgtaatttct
     3361 cgtctggctg gtgaagtatt tggctcggcg gcaacttttg gtgcggtaaa aaaagcctct
     3421 gcaccagggc aaatctcggt aaatgattta cgcacggtat taactatttt acaccaggca
     3481 taagcaataa tatttcggcg ggaacaccct ccccgccgaa cttaaaaaat atatttaacc
     3541 gtatttaata aaaatatttc gtgagtctct gtgcgctaat tctccatctg gcgtagggaa
     3601 aatcacatct gaatcaggaa ttaacaatga aacctgtaaa accacctcgt attaatggac
     3661 gagtgccggt cctgtcggca caggaagcgg tgaattatat tcccgacgaa gcaacacttt
     3721 gtgtgttagg cgctggcggc ggtattctgg aagccaccac gttaattaca gctcttgctg
     3781 ataaatataa acagactcaa acgccacgta atttatcgat tattagtccg acagggcttg
     3841 gcgatcgcgc cgaccgtggt attagtcctc tggcgcaaga aggtctggtg aaatgggcat
     3901 tatgtggtca ctggggacaa tcgccgcgta tttctgaact cgcagaacaa aataaaatta
     3961 ttgcttataa ctatccacaa ggtgtactta cacaaacctt acgcgccgcc gcagcccacc
     4021 agcctggtat tattagtgat attggcatcg ggacatttgt cgatccacgc cagcaaggcg
     4081 gcaaactgaa tgaagtcacg aaagaagacc tgattaaact ggtcgagttt gataacaaag
     4141 aatatctcta ttacaaagcg attgcgccag atatcgcctt cattcg
//
LOCUS       DAZPAC010000298         4131 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZPAC010000298 DAZPAC010000000
VERSION     DAZPAC010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.300"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            115..528
                     /locus_tag="ACHUPM_004131"
     CDS             115..528
                     /locus_tag="ACHUPM_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR0871758.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(573..890)
                     /gene="hspQ"
                     /locus_tag="ACHUPM_004132"
     CDS             complement(573..890)
                     /gene="hspQ"
                     /locus_tag="ACHUPM_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR0871759.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="ACHUPM_004133"
                     /pseudo
     CDS             complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="ACHUPM_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            2233..2511
                     /gene="yccX"
                     /locus_tag="ACHUPM_004134"
     CDS             2233..2511
                     /gene="yccX"
                     /locus_tag="ACHUPM_004134"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR0871760.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="ACHUPM_004135"
     CDS             complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="ACHUPM_004135"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR0871761.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="ACHUPM_004136"
     CDS             complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="ACHUPM_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR0871762.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3794..3881)
                     /locus_tag="ACHUPM_004137"
     tRNA            complement(3794..3881)
                     /locus_tag="ACHUPM_004137"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3845..3847),aa:Ser,seq:tga)
BASE COUNT         1101 a         1047 c         1017 g          966 t
ORIGIN      
        1 atacttacta acatatagac atatttcccg cttttgctct cattcattcg tattagctgc
       61 atggttggca tgtcggcttt gtcgtacact ttgttaaaca gtctggagga aataatgaaa
      121 gaaaccgata ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc
      181 gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac
      241 gtcattccgg tttcgccaaa agttgccggc aaaacgctgc tgggccagca ggggtatggc
      301 acgctggcag acgttccgga aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg
      361 gcgtggggcg tggcacagga agctattgcc attggcgcga aaaccctctg gatgcaactg
      421 ggggtgatta atgagcaagc ggcagtactg gcacgggatg ccgggttgaa tgtggtgatg
      481 gatcgctgcc cggctattga gatccctcgc ctgggcctgg ccaaataaaa aatccccgga
      541 aggcaaaaac cttccgggga ttcgttcagg gatcagttac gcagacgcgg ggcctggagt
      601 tgtttgcgga tggtctgcgc cagctcatcc atggatggct gttcaggatg ttcatcctgc
      661 aattcgctac ttagctgggc ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc
      721 tccatcacta cgtgatacca cggagcggcg cgaagctcgt catttaccgc cagctcatca
      781 ggcgacggtt cagagagcga ataaaccggg tcgatatcca cgaccactcc gagataacct
      841 aacagggaat ggcggacctg ctggccgata ccgaatttgc tggcaatcat agtcacctcc
      901 cgggaaatct ttacctttga tataagggta aaattccaca tttcaagtta catgacgcga
      961 caggcaaacc ctttcagata tagcccttcc ggataggtag cgatcaccgg atgatcggct
     1021 gcctgacgga actgctctat aaattgtaca tcacggccgg catcaattgc ggcatccgcg
     1081 atgattttct gaaataaatc gctggtcatc agaccggaac aggagaaagt caggagaata
     1141 ccgccttcat tcagcaactg aatcgccagc atgttgatat ctttatagcc acgacacgcg
     1201 cccatcaact ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat
     1261 ttttcaccgc gatcgcgata agtacgcagc aatttaaaga catcatcacg gacaaactca
     1321 gccttgctca gatccagttt gttcagctca acgttctgcc gtgcaatatc cagcgcttcc
     1381 taggaggtat caacgctgac aacctggctg caaccgccca tcagtgccga tacggcgaaa
     1441 ccaccggtat aggagaaaca gttcagcaca cgtttatttt caacgtagcg gcgggtagcc
     1501 aggcggctgt ctcgctggtc caggtagtag cccgttttgt gtccgtgctg aatatccacc
     1561 agcagcttca tgccgtgttc ttcaatcggc agcagggcag gcggcaactc gccggtgacg
     1621 aggccctggg tcagctccat cccttctttt ttacgtaccg caacatcgct gcgatcgtaa
     1681 atcgcacatt ccgggtacag cgtttgcagg gcactaatta atgccgcgcg ctgatattct
     1741 gccccagcac tcagcagttg cagcaccaga aaattaccga aacgatcgat agtaataccc
     1801 ggcaggccat cagattctcc ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc
     1861 agtcagtcac gccatttttg tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata
     1921 gactcagacg ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg cgaataagcg
     1981 ccgcgtgcta accattttcc ctgatgatca acaatatcga tggtttcacc gaggctggct
     2041 ttaccttcca tgcgggcaac ggccccggaa aagacccacg gatggcgacg aagtaatgat
     2101 ttttcgcgcc ctttggctaa cactaaacgt acactcataa tttacttttc tgtcgatgca
     2161 aagaaatggg cgtcattgtc cggagttttc catggatttg caacgcgtcc attaaggata
     2221 aggagagcag caatgtcgaa agtctgcata attgcctggg tttacgggcg agttcagggc
     2281 gtaggatttc gctacaccac acagtacgaa gcgaaaagac tgggtttaac cgggtacgcc
     2341 aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg gcaggtggaa
     2401 aaattaatgc agtggttaaa gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc
     2461 agcgagccgc atcatccctc gggggaatta accgattttc gtatccgtta aatacatttt
     2521 accggttttg gcaggccagc aattttggtg gcttgcttag ccggaccttt cggaaacagt
     2581 cggtataaat agcggctgtt acctttttct tcgccaaatt tattcgccat cgcttttacc
     2641 agcatacgaa tcgccggaga agtattgaat tccagataga aatcacgcac aaaacgcacc
     2701 acttcccagt gttccggtga cagcgaaatc ccttcgttct ctgcaatcac cactgccagc
     2761 ggctcactcc actggctgct ttctttgaga tagccttcgg tatccgtttc tatctcttta
     2821 ccttcgaaga tcagcataat tattactacc ttaatcagac tggcggcagt gtaacaaaaa
     2881 acaaagcccc gcataaagcg aggctatgaa agtgttagcg ggtgagatta atcgcggctg
     2941 gcgaagccca gaatgctcag caggctgacg aagatgttgt acagcgaaac atacaggcta
     3001 accgtggcac gaatatagtt cgtttcaccg ccatgaacga tgttgctggt ttcaaacaaa
     3061 atagcgccag aggagatcag aatgaagacc gcgctgatcg ccagatgcag agcaggcagc
     3121 tgcaggaaga tattagcaac cataccaatc agcaccacca caatacccgc catcagcata
     3181 ccgccgagga acgacatatc tttgcgggtg gtcagcacat atgcagagca gcagaagaac
     3241 actaacgccg ttccgcccag tgccatagcg attacgtcac ccattccggc agacagatag
     3301 gtgttcagaa taggtccgag gatataaccc agaaaaccgg taaaggcgaa tgcggagata
     3361 atcccggtcg gcttattcgc cgttttatag gtcaggaaca tcaaaccata catacccacc
     3421 agcgtcagaa tcagacccgg agatggcagc atcagcaccg tactggcagt tgcggtaatc
     3481 gccgaaaagg ccagcgtcag gctcagcaga aaataggtat tacgcagcac cttatgggtg
     3541 ctaagcagtg atgtacggtc atgtgaagaa ctaacaatac gatccattga gtcactctct
     3601 atgacagatg taattaatta agcagcataa tgataatgcg taagggcacc cagaagtttt
     3661 acccatcttt acgcatttga tctggaacag gtttaacagc ggattatcag gtcattaagc
     3721 aaatataacg ccctgagaat ttcgacaggc aaaagaaaaa ggggttagca tttagctaac
     3781 cccttatctt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
     3841 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
     3901 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
     3961 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
     4021 tttactcgtt tagcaaccgg ctaaacatcc ccaccgcccg gcaaaaagaa aaataggtcc
     4081 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa a
//
LOCUS       DAZPAC010000299         4124 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZPAC010000299 DAZPAC010000000
VERSION     DAZPAC010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.301"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..83
                     /locus_tag="ACHUPM_004138"
     CDS             <1..83
                     /locus_tag="ACHUPM_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0871763.1"
                     /translation="FWHDLAALVIAGILASMIVNWLNKRK"
     gene            459..566
                     /locus_tag="ACHUPM_004139"
     CDS             459..566
                     /locus_tag="ACHUPM_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0871764.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(653..2332)
                     /gene="bcsG"
                     /locus_tag="ACHUPM_004140"
     CDS             complement(653..2332)
                     /gene="bcsG"
                     /locus_tag="ACHUPM_004140"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR0871765.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2329..2520)
                     /gene="bcsF"
                     /locus_tag="ACHUPM_004141"
     CDS             complement(2329..2520)
                     /gene="bcsF"
                     /locus_tag="ACHUPM_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR0871766.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2517..4088)
                     /gene="bcsE"
                     /locus_tag="ACHUPM_004142"
     CDS             complement(2517..4088)
                     /gene="bcsE"
                     /locus_tag="ACHUPM_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR0871767.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1051 a         1029 c         1074 g          970 t
ORIGIN      
        1 ccttctggca tgatttagcg gctctggtca ttgctggcat tcttgccagt atgatcgtga
       61 actggctgaa caagcggaag taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt
      121 tgcgcccgga ccaggccgca ggggtgaaac tctgcggcct ttttcgtaag aaaaatgcaa
      181 cgattgcacg cggataatat tctgcggaat gcgcagcaca gagtgaagac gtgacggatt
      241 ccggaacaga aaaacagtcg tagaatcagc ggcaagccgg aaaggttccg gtgaggcgca
      301 atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa cccccgcacg
      361 ttgcaggtat gcacctgaca acatcacggg ggctaatctt gaacccaaac aactcaagaa
      421 tagccgcgaa acgttggcat tacaacacag gcggctatat gacgctcgca gaactgggca
      481 tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc agtatgatcg
      541 tgaactggct gaataagcgg aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca
      601 atttgcgccc ggatcaggcc gcagaggtta aactctgcgg cctttttcgt gcttactgcg
      661 ggtaaggcac ccagtcgccg ccgttcaggc gaacgtacgg tttatcctgg tattgaataa
      721 ctactgcatt tgagttctcg gagaccggtg ctgtttgtgg caacccactg gtgagttttt
      781 tccagtcaac attgtcttcg gtgaaaatct tgccatcaag aacgcgaacc accagatcgg
      841 agatagctag gaagctgctc ggttggtcaa tgacaatcgg tgccccctga tgtggtgcct
      901 tcatgccgaa gaatttcacc ccaacgggga cgtctgtgat agacgggcta gggatatcac
      961 gtaggccaga tacctgcatt ctgtcgccct tcagcgcgcc gccgtgttcc ggcaccacga
     1021 ccaccatcac tttacgaccc gatttctcca gttcagtaaa gaaggcgtcc agttcatcaa
     1081 agaatttctg cgcccgcgct ttgtaatccg ctgttttgct gacccccgga taatggttgc
     1141 cgtcatgcag tggaagcgtg ttgtagaacg tggcactacg gctgttttta tctttttcgg
     1201 taacgtccag ccagcggtta agcacggcgg tatcgtcata aaccggcgaa ccatcaaagc
     1261 ccagcaaaat aaccggcaga tttgtttgat ccatcaattc agtctgcatg ccgccatttt
     1321 cgcgaacttc tttcaaaaaa ccgccgaact ggccgttatg tcccatcatc aggtgctggg
     1381 taaagcccag tttcgaaagg ttatcaaaca gatagcagtc gttatttgcc ggttgataca
     1441 gattagtgtg cgaagtctgc ccgcagctgg cgcgcagtaa acggatcgcc gccgggccac
     1501 tgtaggaggt ggcggagtta aagttcttga actcaatatc gaaatgcgac cacagtggat
     1561 gcgacatcaa cccggcggct tctatatccg accaggaaag cgaacagatg ttaatcacca
     1621 gtagttcaaa tggctgagca tcagcgggca gcgaagacgg gaaggtcgat ttacgtttcg
     1681 cctccgcgtt atagaaatta ttcagccagg cgttaaggtt cgccgttgtt ggcggtgcag
     1741 tttgtgcggg catatcaccc actaccgatg cgccacccgt cgccgcaacg gttgccgctg
     1801 cgttaccacc cgtcgttgtt acagtggtcg tcggttgtcc ggctggccac aaggagaaac
     1861 ttggtcccgc cagggtaagt acgttcagcc atagcagtat ggcaaccaca aaaacggtaa
     1921 tgcgaatcca ttgtgacagg aataaccagg ccactaataa aacaaaaatg gccccaatca
     1981 tctgccagtt aataaagcgt gtgacaaggt cgattaaata atcggtactg aaccccgcca
     2041 cctgcgaacc ctggctcatt atgctttccg ggccaggcaa ccaggtgtca tgccagaaca
     2101 aagcaaagcc gatcggcagg gcaatccagt ggcgcaagcg atgcaggctg tagcgcggaa
     2161 tgggcatcag cagaaacgtg gcaaacacca aattgaggag cggatggaag ttaagatatc
     2221 ccgcccacaa caggccgaac ttaaccagaa aatagaagtt ccagccggaa aggccgcgcc
     2281 agtattgcca gagggaagaa ggcatggcgg tattttgcgt aaattgagtc attttttggt
     2341 tgccctggct ttttccgtgc ggcgtaacgt cccggccggt ttaacataac gaggtttagc
     2401 aaagaataaa cgtaaggtgt cgcgaatgcg tcgcaaaaag tgccgcgcca gatagcccag
     2461 cgggaaaaat atcagtgcgc aaacgacaat aatttcaatg atatcgctga tggtcatcat
     2521 gatgagcgct ccacagcatc atctaacagt cgcatgggtt ctggtattcg tcgccagtgg
     2581 cgaccatcgt gctcggcatt gatgaccggt ttagaacttt gcgttaaagg cagcggcatg
     2641 ccccattgtt ctggggcaag caagcgcatc tgcaccagct cggcactgat ttgatcatct
     2701 tcaaaccaga ccatacggtt tgagaaaatg tcgccagtag gcaatgggaa aatatgattc
     2761 aacgcggtat ccagatcgtt aatccgacag aatgagagaa acagcaccag ccgattaccg
     2821 ccaatggtca tgatatcgcc ggtacggtta gggcgacaca gcgtcagggc ttgttcaacg
     2881 cggatacccg gtaccggacg tagggcaacc agaacgcctt taccgtgggc aggtaataga
     2941 gggttattca tcatgttgtt gacggcatta cagaacacat cccacttctg gaaaccacgc
     3001 agtttgagcg gctgggtcat tgacagcaag gtagtgatat cttccggcac atagcgacta
     3061 aacttctgcc cttgcacgct ttcgatcatc gtcagacaac gggagagtgg cgcattccac
     3121 ggaataacca tatttgcacc gcaggccaat aacaaacgtt catcggtggc gcgcaggcta
     3181 gcggtatttt cccgcacgag gattttcatc gcactaccgc gctggcgacg cagggtatga
     3241 atgctgcggg ccagtggctc gatttgcgca ttttgctgta aagaaaagac caccgtcgcc
     3301 gcctgagcgg tacgggcttc attgaacagg acttcgttat tgttgaacag ttgccagtgt
     3361 tccgatagcg gcggcgcacc ttccagtaca gcaacattac tcagaatgcg tttttcgtcg
     3421 ctgcgtggtt ggatctccgc ctcttcgctt tgaactaatg tccagatacc attttgttgc
     3481 tgaacgctaa gctgctgacg ggcgctgacc cctttttcgt tgcaccagaa ggcaatatcc
     3541 agcaaatgtt ggtcaccctg aaaacgcaaa ctggcaagac caaaaagtga acggtattcc
     3601 tcaagcaaca atgaaaattg tttatcgtta ttatttccgg gattaattac caaaagcgaa
     3661 caatggttta acctgctcca tttattcatt ttatccaacc atgagcgaag ccgctcggca
     3721 ggaatgtttt gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt atggggatca
     3781 atagaacact gcaaatcacg ggccaaatag tatagacctt tttcatgatt tagcattgaa
     3841 aataatttta ttttttccgg cccttgagaa tcatctaatt gaaagatttt cgccggatcg
     3901 ctgtccatgc taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat tgtttgattc
     3961 gccagactga tagcatcttc atggcgatcg acgttaaacc accagacgcc gcctgctggc
     4021 atatgtcgca gctcatccca taatgatgag ataccgatag agaatacagg gtccacaatg
     4081 tccctcatgt caccattttg tctgattatc agtttactag cgaa
//
LOCUS       DAZPAC010000300         4119 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZPAC010000300 DAZPAC010000000
VERSION     DAZPAC010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.302"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            226..3534
                     /gene="icsA"
                     /locus_tag="ACHUPM_004143"
                     /gene_synonym="virG"
     CDS             226..3534
                     /gene="icsA"
                     /locus_tag="ACHUPM_004143"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFR0871768.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGNGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVVGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQNEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            3678..3996
                     /locus_tag="ACHUPM_004144"
                     /pseudo
     CDS             3678..3996
                     /locus_tag="ACHUPM_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT         1245 a          713 c          962 g         1199 t
ORIGIN      
        1 actatcatag taaaattatt aatttattat cgaacatata gctttccccc tctttttttc
       61 aaagcaagac acaggtaaat ttctcccgtt gcattgatat ataacacagc tctcatgttt
      121 tggttgaggc tttgtttaat atatttttgc atatatcgtc cctttattcc ggataaaacg
      181 gaatcttttt aggggtttat caaccactta ctgataatat agtgcatgaa tcaaattcac
      241 aaattttttt gtaatatgac ccaatgttca cagggggggg ccggagaatt acctacggta
      301 aaggaaaaaa catgcaaatt gtctttttct ccttttgttg ttggtgcatc cctgttgctc
      361 ggggggccaa tagcttttgc tattcctctt tcgggtactc aagaacttca tttttcagag
      421 gacaattatg aaaaattatt aacacctgtt gatggacttt ctcccttggg agctggtgaa
      481 gatggaatgg atgcgtggta tataacttct tccaacccct ctcatgcatc tagaactaag
      541 ctacggatta actctgatat tatgattagc gcaggtcatg gtggtgctgg tgataataat
      601 gatggtaata gttgtggcgg taatggtggt gactctatta ccggatctga cttgtctata
      661 atcaatcaag gcatgattct tggtggtaac ggcggtagcg gtgctgacca taacggtgat
      721 ggtggtgagg ctgttacagg agacaatctg tttataataa atggagaaat tatttcaggt
      781 ggacatggtg gcgatagtta tagtgatagt gatgggggga atggaggtga tgccgtcaca
      841 ggagtcaatc tacccataat caacaaaggg actatttccg gtggtaatgg aggtaacaat
      901 tatggtgagg gtgatggcgg taatggaggt gatgccatca caggaagcag cctctctgta
      961 atcaataagg gcacgttcgc tggaggcaac ggaggtgctg cttacggtta tggttatgat
     1021 ggctacggtg gtaatgctat cacaggagat aacctgtcta taatcaacaa tggagctatt
     1081 ttaggcggta atggtggaca ttggggggat gctataaatg gtagcaatat gaccattgct
     1141 aatagcggat atataatttc aggtaaagaa gatgatggaa cacaaaatgt agtaggtaat
     1201 gctatccaca tcactggtgg aaacaattca ttaatactcc atgaaggttc tgtcattact
     1261 ggtgatgtac aggttaacaa ttcatccatt ctgaaaatta tcaacaatga ttacactggg
     1321 accacaccaa ctattgaagg tgatttatgt gctggtgatt gtacaactgt ttcactatca
     1381 ggtaacaaat tcactgtttc aggtgacgtt tcttttggtg agaacagttc tttaaattta
     1441 gctggaatca gtagtctgga agcttctgga aatatgtcat ttggcaacaa tgtaaaagtg
     1501 gaagctatta taaataactg ggcgcagaag gactataaac tgctaagtgc agataaaggg
     1561 ataacaggtt tcagtgtttc taatatatct atcatcaatc cgttactcac tactggtgct
     1621 attgactata caaaaagcta tatcagtgac cagaataaat tgatctacgg tttgagctgg
     1681 aatgatacag atggcgacag tcatggagag ttcaatctga aagaaaacgc tgaacttact
     1741 gttagtacta ttctggcaga taatctcagc catcataata taaatagctg ggacggaaaa
     1801 tccctaacaa aatcagggga gggaactctc attttggcgg aaaaaaatac ctactctggt
     1861 ttcaccaaca tcaatgcagg cattctaaaa atggggacag ttgaagctat gacacgtacc
     1921 gctggtgtta ttgttaataa aggtgctacc ttgaattttt caggcatgaa ccaaactgtt
     1981 aacactttat taaatagtgg gactgtgcta atcaataata ttaatgcccc ttttttgcct
     2041 gaccccgtca ttgtcacagg taacatgact ctggagaaaa acggtcatgt tattctcaat
     2101 aatagttcgt caaatgtcgg tcagacctat gttcagaaag gtaattggca tggaaagggc
     2161 ggaatattat ctttgggcgc ggttctcggc aatgacaaca gtaaaactga ccggctggaa
     2221 attgcaggcc atgcgtctgg tattacctat gttgcagtga caaatgaggg aggctctgga
     2281 gataaaactc ttgaaggtgt tcaaattatt tcgacagatt cttctgataa gaatgctttt
     2341 attcagaaag gccgtattgt tgctggtagt tatgactatc gcctgaaaca gggcactgta
     2401 tctggactga ataccaataa gtggtatcta actagtcaga tggataatca agaatcaaaa
     2461 cagatgagca atcaagagtc tactcaaatg agtagtcgcc gagctagttc acagcttgta
     2521 tcttcactta atttgggtga aggtagtatt cacacatggc gccctgaagc tggcagttat
     2581 attgctaacc tgatagcaat gaacacgatg tttagtcctt ctctctatga ccgacacggt
     2641 agcactattg ttgatcctac tacaggtcag ctcagcgaaa ccaccatgtg gattcgtact
     2701 gttggtggac ataatgagca taatttagct gatagacaat taaaaaccac agctaacagg
     2761 atggtttatc agattggtgg agatattttg aagacaaact tcactgatca tgatggcttg
     2821 catgtgggta ttatgggagc ttatggatat caggatagca aaactcataa taagtatact
     2881 agttatagtt cacgaggaac tgtgagcggt tatactgccg gtttgtacag ttcttggttt
     2941 cagaatgaaa aagaacgaac aggtctatat atggatgctt ggttgcagta cggttggttt
     3001 aataatacag tcaaaggaga tgggttaact ggtgagaaat attccagcaa aggaataaca
     3061 ggagctttgg aagctggcta tatctaccca accatacgct ggactgctca taataatatt
     3121 gacaacgcat tgtatctcaa tccacaagtc cagataacta ggcatggggt aaaagcaaac
     3181 gactatattg aacacaatgg cactatggtc acatcctctg gggtcaataa tattcaagca
     3241 aaattgggat tgcgtacatc cttaattagt cagagttgta tcgataagga gactcttcgt
     3301 aagttcgaac catttttgga agtgaattgg aaatggagct caaagcaata tggtgtaatt
     3361 atgaatggca tgtcaaatca ccagataggc aaccgtaatg tgattgaact caaaactggt
     3421 gtgggggggc gtcttgcaga taacctaagc atctggggaa acgtatctca gcaattgggt
     3481 aataacagtt acagagacac ccaaggtatt ttgggtgtga aatatacctt ctgataataa
     3541 aaatgcaggg cgtgttgatg tcctgcattt ctctgggacc caccgtgtaa aagctcggtg
     3601 ggtttttgtg cacatgatgg tatgcctgcg atcaatgcca aacgtgttta ccagatcatg
     3661 cgccagaatg cgctgttgct tggcgttaca caggaattgc attagaaagc gatgaacgaa
     3721 tgctaccgcc aaatattcgt ggttatgcgc ctcagattac tggtattgca gaaactaatg
     3781 cacgtgttgt tgtatctcaa caaggtcgag taatatatga ctctactgta ccagctggaa
     3841 ctttttctat tcaggacttg agtagttctg tacgaggaat attggatgtt gaaatttttg
     3901 agcaaaatgg taaaagaaaa catttccaag tagaaatgtg caggtgtgcc tttcttatcc
     3961 agacttggtc agagtaggta taaattagta acaggtctac ctaaaacaaa taataaagca
     4021 actggtgatg catttttttc ggtgaagatc ctacggggtc ctgagcccgg tgaagtggcg
     4081 aggcaaatta catggggaag cgtacagcct gaactgaac
//
LOCUS       DAZPAC010000301         4118 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZPAC010000301 DAZPAC010000000
VERSION     DAZPAC010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.303"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            154..762
                     /locus_tag="ACHUPM_004145"
     CDS             154..762
                     /locus_tag="ACHUPM_004145"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR0871769.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            860..2251
                     /gene="selA"
                     /locus_tag="ACHUPM_004146"
     CDS             860..2251
                     /gene="selA"
                     /locus_tag="ACHUPM_004146"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR0871770.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2248..4092
                     /gene="selB"
                     /locus_tag="ACHUPM_004147"
     CDS             2248..4092
                     /gene="selB"
                     /locus_tag="ACHUPM_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR0871771.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          929 a         1038 c         1216 g          935 t
ORIGIN      
        1 tgagatatcc gtcagataga aacaattagg aaaatattta tatcaccatg ataaatatgt
       61 gatttatttg gaagtaacaa accatcaaca ttttctgccc tttttcccat caccaggcca
      121 tactgatatt tttcctcgca caaaggactc ggcatgaaac tcgtcggtag ctacaccagc
      181 ccgtttgtac gcaaactttc tattctgttg ttagaaaagg gcataacttt cgaatttatt
      241 aatgaactgc cctataacgc ggacaacggc gtggcgcaat ttaacccgtt aggaaaagtg
      301 ccggtgctgg tgaccgaaga gggcgaatgc tggtttgatt cgccgatcat cgctgaatat
      361 attgagttaa tgaatgtcgc tccggcgatg ttgccgcgcg atccgctgga gtcgttgcgg
      421 gtgcgcaaaa ttgaggcgct ggcggatggc attatggatg ccgggctggt atcggtgcgt
      481 gaacaggcgc gtccggcggc gcagcagtct gaagatgaat tgttacgcca gcgggagaaa
      541 atcaaccgca gtctggatgt gctggaagga tatctggtcg atggcacact caaaaccgat
      601 acggtcaatc tggcgactat cgccattgcc tgtgctgtcg gatatctcaa tttccgccgc
      661 gttgcgccag gttggtgtgt cgaccgccca cacttagtca aactggtcga aaacctgttt
      721 agccgcgaaa gttttgctcg caccgaaccg ccaaaggctt gatgcgcgat atgtcctcct
      781 gacccatctc acgttacaat ccgtggttat gttaaacgcc cttctccgtg tgagagggcc
      841 ttgatcagcc aggtttccta tgacaaccga aacgcgttcc ctctatagtc aacttccggc
      901 tattgatcgc ttattgcgcg atagctcctt cctttctttg cgtgatactt atggtcacac
      961 ccgcgtggtg gaattgttgc gtcagatgct cgacgaagcg cgagaagtga ttcgtgacag
     1021 ccagacgctt cctgcgtggt gtgaaaactg ggcgcaagaa gtcgatgccc ggttgacgaa
     1081 agaggcgcag agcgcgctgc gtccggtgat caacctgacg ggaaccgtgc tgcataccaa
     1141 ccttggacga gctttacagg cggaagccgc ggtggaagcc gttgcgcagg ctatgcgttc
     1201 gccagtgacc ctcgagtacg atctggacga cgccggacgc ggacatcgcg atcgggcgct
     1261 ggcgcaactg ctgtgccgta ttacgggggc ggaagatgcc tgtatcgtca ataacaatgc
     1321 ggcggcggtg ttattgatgt tggcggccac tgccagcggg aaagaggtgg tagtatctcg
     1381 cggcgaattg gtggagattg gcggcgcgtt tcgtattccc gatgttatgc gtcaggcagg
     1441 ctgcacccta cacgaagtag ggaccaccaa ccgcacgcac gcgaatgatt atcgtcaggc
     1501 ggtgaatgaa aataccgcac tgttgatgaa agtacatacc agtaactaca gcattcaggg
     1561 gttcaccaaa gcgatagatg aagcggaact ggtggcgctc ggcaaagagc tggatgttcc
     1621 cgtagtgact gatttaggca gtggctcgct ggtcgatctt agccagtacg gtttgccgaa
     1681 agagccaatg ccgcaggagt tgattgcggc gggcgtcagt ctggtgagct tctccggcga
     1741 caagttgtta ggcgggccgc aggcagggat tatcgttggt aaaaaagaga tgatcgcccg
     1801 cctgcaaagc cacccgctga agcgtgcatt acgcgcggat aaaatgaccc tcgcggcgct
     1861 ggaagccacg ttgcgtcttt atttacaccc tgaagctctg agtgaaaaat taccgaccct
     1921 gcgcctgctt acccgcagcg cagaggtcat tcaaatccag gcacaacgtt tacaggctcc
     1981 ccttgccgca cattacggcg cggagtttgc ggtacaggtt atgccatgtc tttcgcagat
     2041 tggcagtggt tcgctgccgg ttgatcgcct gccgagcgcg gcattaacgt ttacacccca
     2101 tgatggacgc ggtagccacc ttgagtcatt agccgcccgc tggcgtgaat tgccagtgcc
     2161 gatgattggt cgtatttatg acggacgatt gtggctggat ttacgctgcc ttgaagatga
     2221 gcaacggttt ttggagatgt tgttgaaatg attattgcga ctgccggaca cgttgaccac
     2281 ggcaaaacaa ccttattgca ggtgattact ggcgtaaatg ctgaccgtct gccggaagaa
     2341 aaaaagcgcg gcatgaccat cgatctcggc tatgcctact ggccgcagcc ggatggtcgc
     2401 gtgcctagtt ttatcgacgt acccggtcat gaaaagtttc tttccaacat gctggcgggc
     2461 gttggtggta tcgatcacgc gctgttggtg gtggcgtgcg atgacggcgt gatggcgcag
     2521 acccgtgagc atctggcgat tttgcagctg accggtaacc cgatgctgac agtggcgctg
     2581 accaaagccg atcgcgtgga cgaagcgcgt gttgatgagg ttgaacgcca ggtaaaggag
     2641 gtgctgcggg aatacggttt tgctgaggca aaactgttta tcaccgcagc aaccgaaggt
     2701 cggggaattg atgccctgcg cgagcatctg cttcagttac caggacgtga acacgccagc
     2761 caacatagtg tccgcctcgc gatcgaccgc gcatttaccg taaaaggtgc cggtctggtc
     2821 gtcaccggta cggcgttaag cggggaagtg aaggtaggcg attcactctg gctgactggt
     2881 gtaaataaac cgatgcgtgt acgtgcgctg catgcacaaa accagccaac agaaaccgcc
     2941 catgccgggc agcgtatcgc gcttaacatc gcgggtgatg cggaaaaaga gcagattaac
     3001 cgtggcgact ggctgcttgc cgatgcgccg ccagagccgt tcacacgggt gattgtcgag
     3061 cttcaaaccc atacgccgct gacccagtgg cagccgctgc atattcacca cgccgccagc
     3121 cacgttacgg gacgcgtttc gctgctggaa gataaccttg ccgagctggt gttcgacacg
     3181 ccgctgtggc tggcggataa cgatcgctta gtgctgcgcg atatctcggc ccgcaacacg
     3241 ctggcggggg cgcgtgtggt gatgcttaac ccgccgcgtc gcggtaaacg taagccggaa
     3301 tatctgcaat ggctggcgtc tcttgcacgg gcgcagagcg atgccgatgc gttatctgtt
     3361 catctggaac gcggcgcggt taaccttgcg gatttcgcct gggcgcgcca gctcaacggc
     3421 gaagggatgc gcgaattgct gcaacagcct ggctatattc aggctggtta tagcttgttg
     3481 aatgcgccgg ttgccgcccg ctggcagcgg aaaattctcg acacattagc gacttatcat
     3541 gagcaacatc gcgatgaacc tggtcctggg cgcgaacgtc tgcgacgtat ggcgttgcca
     3601 atggaagatg aagcgctggt actgttgctg attgaaaaga tgcgcgaaag cggcgacatc
     3661 ctcagtcatc acggttggct gcatctgccg gatcacaaag cgggcttcag cgaagagcag
     3721 caggccatct ggcaaaaagc agagccgctg tttggtgacg agccgtggtg ggtgcgtgat
     3781 ctggcaaaag agacgggtac cgacgagcag gcaatgcgcc tgacgctacg ccaggcggcg
     3841 cagcaaggaa taattaccgc gatcgtcaaa gatcgttatt accgtaacga tcggattgtc
     3901 gaatttgcca atatgatccg cgatctcgat caggagtgtg gttcaacctg cgcggcggat
     3961 ttccgcgatc gcttaggcgt aggccgaaag ctggcaattc agattctgga atattttgac
     4021 cgcattggct ttacgcgtcg tcgtggaaat gatcatttat tgcgcgacgc attattattt
     4081 ccggaaaaat aaggaaatga ttaattaatt tttaaaat
//
LOCUS       DAZPAC010000302         4107 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZPAC010000302 DAZPAC010000000
VERSION     DAZPAC010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.304"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..759)
                     /gene="nlpA"
                     /locus_tag="ACHUPM_004148"
     CDS             complement(<1..759)
                     /gene="nlpA"
                     /locus_tag="ACHUPM_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFR0871772.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVALGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
     gene            981..1274
                     /gene="yicS"
                     /locus_tag="ACHUPM_004149"
     CDS             981..1274
                     /gene="yicS"
                     /locus_tag="ACHUPM_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR0871773.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(1315..2495)
                     /gene="nepI"
                     /locus_tag="ACHUPM_004150"
                     /pseudo
     CDS             complement(1315..2495)
                     /gene="nepI"
                     /locus_tag="ACHUPM_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2706..3158)
                     /locus_tag="ACHUPM_004151"
     CDS             complement(2706..3158)
                     /locus_tag="ACHUPM_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR0871774.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(3211..>4107)
                     /gene="adeQ"
                     /locus_tag="ACHUPM_004152"
                     /gene_synonym="yicO"
     CDS             complement(3211..>4107)
                     /gene="adeQ"
                     /locus_tag="ACHUPM_004152"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR0871775.1"
                     /translation="LESLPGIGLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGI
                     LGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSG
                     ALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAG
                     AFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALI
                     FVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRW
                     RDLNLCVVVVASLFALKIILVD"
BASE COUNT         1211 a         1080 c          911 g          905 t
ORIGIN      
        1 tgattgataa gattgcagaa attccttcac gttttctgca ttcttattat cttcccgtgc
       61 caccaaaata ttcacatacg gcgaattctt atcttcaata aatacgctgt cgtgcaccgg
      121 agaaagccct gtctgctgaa tgtaagtggt gctgataatc gctacatcaa ctttgggatc
      181 atccagtacg cgcgggagct gcgccccctc gagttccata atctgcaaat gacgaggatt
      241 atcggttata tccagcgcgg taggtaataa cccttttccc tctttcagag taattagctt
      301 ctctttttgc aacagtaaaa gtgcgcgacc aaggttggtc gggtcgttgg ggatcgccac
      361 tgtcgcgcct tcttttattt gcgccacggt tttgattttt ttggaataac ccgccatagg
      421 gaagacaaac gtatttccca gcgctaccag tttataacca tgcgcctgat tatcttgttc
      481 aagaaagggt cgatgctgga agacattcgc atcaagttcg ccatgatttg tcgcatcgtt
      541 cggtagcaac gaaccgctaa atcctaccag ttcaacatcg aggccatatt tctcttttgc
      601 caccttttta gcgacttccg cgacatcttg ttctgcgcca tttataacgc caactttaat
      661 gtgttttgca tcgctgctac tctggtcgca acctgccagc agaattccgg ccagcaataa
      721 tgcggcccct gtccgtagat gatgtgttgt cagtttcatt tttttatcct tttattaatg
      781 attacctgac tatagcggta aggtcgctgc ggtttaaaaa acgaaacgct atcgataaga
      841 ataaaaagga ataaaagtgg aatataaagt ctggcacttc ttactgacca cgcaagcgcg
      901 tttcgtacag catgatgaga gcgatgaaag caaactacac ttatgcttca ttcgatacac
      961 atttgtaaaa ggttgaatcc atgaaaccaa cgacactact gcttattttc acttttttcg
     1021 ctatgccagg catcgtatac gccgagtcgc cttttagctc actgcagtcc gcaaaagaga
     1081 aaaccacggt attacaagat ttacgtaaaa tttgcacgcc acaggcgtca ttatcagatg
     1141 aagcgtggga aaagttaatg ttgtcagatg aaaacaataa acaacatata cgcgaggcca
     1201 tcgtggcgat ggaacgtaat aatcagagca attattggga agcgttgggt aaggtagaat
     1261 gcccggatat gtaagaacag caacaactac tgaccaacgc ttctttacct gatatcagga
     1321 tttcttcatt ttcacctttg cagtaaccaa caatgcagtc agcaacatca atgtgccaga
     1381 aaacatcaac ggcgaagtca gaccaatatt atccagcgca taaccgccga ttgccgcgcc
     1441 acaagctgaa taaccgccac ctgaatagac ccggcttttt ctgcctgatc ggccagtgag
     1501 cgggtgatcc acgttgacca gccgacggga accaatgcaa aagttagccc ccagataatc
     1561 gccacgccgg tagcaacgat tttatcgctt ccccacaacg tcagcaccaa cgcactcaca
     1621 gccagtatta acggcgcgcc tgctaaggcc agttttaccg aacgtttaag aatgaatgac
     1681 gaaagcgacg taccgataaa gctggcaatg ccaaaactca acagcaccag cgttaagcca
     1741 tccacgctga atcccgccag gttcatatac accgggcgaa tatatgtgaa gaaagcaaac
     1801 tgcccggcga aagacatgaa gatggcgatc atccctgcca tcacacccgg acgttgtaat
     1861 aagcggaaag tattttgttt ctgatgcgag ggttcgcctg gcagtgaagg caatgatttg
     1921 ataatccaga aaatacacag cacgcccatc accgccgccg cattaaagac attgcgccaa
     1981 ccgataagct cgcctaaaaa actgcccaac ggcgcggcaa tcaccagcgc aatagaaacc
     2041 gcgccgaaga tcaccgacag cgccttcggc accgtacgcg gcggcaccag acgcatggtc
     2101 agcgacgccg acatcgccca gaacccgccc agcgccagcc ccagacaggc acgaccgatt
     2161 aaaagcaaac tgaatgagtt agcaaaggaa accagcaagc aggagagcgt cagcaaaacg
     2221 gcaaacaaaa taacaacgtt gcggcggtca gtagcctgaa ttgtctgggt aataaacaaa
     2281 ctggcaaaca ttgccacaaa ggcggtcacg gtcaccgatt gcccggcaac cccttccgaa
     2341 atgcctaaat cctgggccat tggcgtcaac aaactgacgg gcaaaaactc aacgataatc
     2401 agacaggcga cacaaaacgc caccgagaaa acggctgacc agttcggtcg ggtgatggca
     2461 tccgcgccgc ggttttcggc aataaattca ctcatggtgt tacccgtggc atgtttcagg
     2521 ggaaaagccg accactttaa cattgaaatg tgtgacgcat ttaacgtttt tgcaactttt
     2581 acggtgttgc ttgagctgga gttaagatcg aaacggaaca gggcaaagat taccggatgc
     2641 ggcgtgaacg tcttatccac ccgacaggtc aggcgcggtc tgtaggctga taagacgaag
     2701 cggttttaat gcagccagtg aacgccctcg gcaggctgaa agaacgcgtt atagcgcatc
     2761 tgaaaagcat taatatcctg catttcaggt tcggtttcgc gcagaaaacc cagcgccagc
     2821 ctgacctgag catcggtaat cggcgcggaa aggcgggctt tttgtaaaat ccgatgccat
     2881 tgcagcaggt tctgttcgct accatcgaca atgacaattt gccagatgag caacacctgc
     2941 gcggcgtttt gcaggtgcga ctcatccggg cgatgcaaat aacgtaaaaa ttcgtcaccg
     3001 gctgactttc ccatttgatc gaccatcgac tccacgggta cgacatcgat attcagccga
     3061 tcactgagac ggcgaatcgc ttttctggcc ccggatgtca gcacccgaaa accaaccaca
     3121 aacactaccg ccagcgtggc gagcattatc cagatcatga gttctcctgc gttatcagaa
     3181 ctcatgatat ctggaaaccc tcgccagtta ctaatccacc agaataatct tcagtgcaaa
     3241 cagagatgcg accaccacca cacacaggtt cagatcgcgc cagcgcccgg tgcacacttt
     3301 catgatgcag tacgacataa agccgagtgc aatcccttcg gtgatcgaga aagtaaaggg
     3361 catcatcacc gtggtaataa acgcaggcac cgattcggta aaatcatccc agttaacgcg
     3421 cgccaggctc gaagtcatca gcacgccaac aaagattaac gctccggcgg ttgcgtaagg
     3481 aggaaccatc gccaccagcg gggagaagaa cataaccaac aggaacataa cgccaaccac
     3541 aaccgcagtc agccccgtgc ggccaccgac tgccacacca gaagtacttt caatataggc
     3601 agtaacagac gaggtgccga taaacgcacc cgccaccgaa ctgacgctat caacatacag
     3661 cgccttattc atattgggga atttaccgtt accatctatt aagcccgctt tatcagttac
     3721 accaattaat gttcctgatg aatcaaatag gttgatcagc ataaaggaga aaatgatacc
     3781 ggcgagttca agtgttaacg cgccgctcaa atctacttca ccaatgacgc cgctaatatc
     3841 aggcggaatg gaatagacgc cgctaaaatg aacatcaccg aaaaataatc cacagcaaga
     3901 cgtcaccaca atagaaacca gcaccgcggc atgaaaatga cgtgatgaca acacggttat
     3961 aataaaaaaa cctaaaatac ctaacaacac gccgtgagaa cttaaatcgc caatcatcac
     4021 cagcgtgtct ttattggcga caataacgcc agtatttttt aatcccatta aggcgataaa
     4081 taatccaatt ccgggtaatg actccaa
//
LOCUS       DAZPAC010000303         4094 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZPAC010000303 DAZPAC010000000
VERSION     DAZPAC010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.305"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..523
                     /gene="fliD"
                     /locus_tag="ACHUPM_004153"
     CDS             <1..523
                     /gene="fliD"
                     /locus_tag="ACHUPM_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR0871776.1"
                     /translation="YKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAF
                     KTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKG
                     YLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTS
                     TYLSQQFTAMSNS"
     gene            538..948
                     /gene="fliS"
                     /locus_tag="ACHUPM_004154"
     CDS             538..948
                     /gene="fliS"
                     /locus_tag="ACHUPM_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR0871777.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            948..1313
                     /gene="fliT"
                     /locus_tag="ACHUPM_004155"
     CDS             948..1313
                     /gene="fliT"
                     /locus_tag="ACHUPM_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR0871778.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            1391..2878
                     /gene="amyA"
                     /locus_tag="ACHUPM_004156"
     CDS             1391..2878
                     /gene="amyA"
                     /locus_tag="ACHUPM_004156"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR0871779.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(2912..3325)
                     /gene="yedD"
                     /locus_tag="ACHUPM_004157"
     CDS             complement(2912..3325)
                     /gene="yedD"
                     /locus_tag="ACHUPM_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR0871780.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            3512..>4094
                     /locus_tag="ACHUPM_004158"
     CDS             3512..>4094
                     /locus_tag="ACHUPM_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HFR0871781.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQAR"
BASE COUNT         1044 a         1001 c         1056 g          993 t
ORIGIN      
        1 atataaagaa gtagacccag gtgctgaagc acaagataaa aataatggtg cacttctcgg
       61 agacagcgtt gtacgtacaa ttcaaagcgg catccgcgca caatttgcaa acggtgccag
      121 cgacagtgcc ttcaaaacat taaacgagat cgggattaag caggacggca cgacgggtaa
      181 gctgaaaatt gatgatgata aactcaaaaa agtcctgaat gaaaatacag catccgtacg
      241 tgaattgctg gtaggcgatg gtaaagaaac aggaattacc accaaaattg ccaccgaggt
      301 aaaaggttac cttgccgatg atggcattat tgatagcgca caggacagta ttaacgccac
      361 actgaaaaaa ctgaccaaac agtatctatc cgtcagtgcc agcattgatg acaccgtggc
      421 gcgttacact gcacagttta cgcaattaga taccatgatg agcaaactga ataacaccag
      481 tacctatctc agccaacaat ttactgcaat gagtaattcc tgatcacgga ggtcagcatg
      541 tataccgcga gcggctcaaa agcctatgca caaattggcg tcgaaagcgc cgtaatgagc
      601 gccagccagc aacagctggt caccatgcta tttgatggag tgctgagcgc actggttaga
      661 gcgcgcctgt ttatgcagga caacaatcag caaggcaaag gcgtctcttt gtcaaaagcg
      721 atcaacatca ttgagaacgg gctgcgggtg agtcttgatg aagagagcaa agacgaacta
      781 acccaaaact tgattgctct ttatagctat atggtcaggc gcttgctgca agccaattta
      841 cgcaacgatg tctccgcagt cgaagaagtg gaagcattaa tgcgcaatat tgccgatgcc
      901 tggaaagagt ctttactctc cccttctttg attcaggacc cagtctgatg aaccatgcac
      961 cgcatttgta tttcgcctgg caacaactcg tcgataaaag ccagctcatg ttacgcctgg
     1021 caacggaaga acaatgggac gaactcatcg ccagcgaaat ggcgtatgtg aatgcggtgc
     1081 aggagattgc acatctgact gaagaggttg ccccgtccac cacgatgcag gagcagctcc
     1141 gcccgatgct gcgcctgatt ctcgacaacg aaagcaaggt aaagcagtta ttacagattc
     1201 gtatggatga actggcgaaa ctggtcggtc agtcatcggt gcaaaaatca gtgttaagtg
     1261 cctatggaga tcagggcggc tttgtgctgg ctccgcagga taacctcttt tgaatcttaa
     1321 cgagccgatg gctcgcgaat aatccgatta cggctacgct tctaatgttc cccttgaatg
     1381 gagtcgaaga atgcgtaatc ccacgctgtt acaatgtttt cactggtatt acccggaagg
     1441 cggtaagctc tggcctgaac tggccgagcg cgccgacggt tttaatgata ttggtatcaa
     1501 tatggtctgg ttgccgcccg cctataaagg cgcatcgggc gggtattcgg tcggctacga
     1561 ctcctatgat ttatttgatt taggtgagtt tgatcagaaa ggcagcatcc ctactaaata
     1621 tggcgataaa gcacaactgc tggccgccat tgacgctctg aaacgtaatg acattgcggt
     1681 gctactggac gtggtagtca accacaaaat gggggcggat gaaaaagaag ctattcgcgt
     1741 gcagcgtgta aatgctgatg accgtacgca aattgatgaa gaaatcattg agtgcgaagg
     1801 ctggacgcgt tacaccttcc ccgcccgtgc cgggcaatac tcgcagttta tctgggactt
     1861 caaatgtttt agcggtatcg accatatcga aaatcctgat gaagatggca tatttaaaat
     1921 tgttaacgac tacaccggcg aaggctggaa cgatcaggtt gatgatgaac tcggtaattt
     1981 cgattatctg atgggtgaga atatcgattt ccgcaatcat gccgtgacgg aagagattaa
     2041 atactgggcg cgctgggtga tggaacaaac acaatgcgac ggttttcgtc ttgatgcggt
     2101 taaacatatt ccggcgtggt tttataaaga gtggatcgaa cacgtacagg aagttgcgcc
     2161 aaagccgctg tttattgtgg cagagtactg gtcgcatgaa gtcgataaac tgcaaacgta
     2221 tattgatcag gtggaaggca aaaccatgct gttcgatgcg ccgctgcaga tgaaattcca
     2281 tgaagcatcg cgcatgggac gcgactacga catgacgcag attttcacgg gtacattagt
     2341 ggaagccgat cctttccacg ccgtaaccct cgtagccaat cacgacaccc aaccgttgca
     2401 agccctcgaa gcgccggtcg aaccgtggtt taaaccgctg gcgtatgctc taattttgtt
     2461 gcgggaaaat ggcgttcctt cggtgttcta tccggacctc tacggtgcgc attacgaaga
     2521 tgtcggtggt gacgggcaaa cctatccgat agatatgcca ataatcgaac agcttgatga
     2581 gttaattctc gcccgtcagc gtttcgccca cggtgtacag acgttatttt tcgaccatcc
     2641 gaactgcatt gcctttagcc gcagtggcac tgacgaatat cccggctgcg tggtggtcat
     2701 gtcgaacggg gatgatggcg aaaaaaccat tcatctggga gagaattacg gcaataaaac
     2761 ctggcgtgat tttctgggga atcggcaaga gagcgtagtg accgacgaaa acggcgaagc
     2821 gaccttcttt tgcaacggcg gcagcgtcag cgtgtgggtt atcgaagagg tgatttaaat
     2881 tcatccccgg cggcaagccg gggagatttc attacggcag tggcgtcggt aacggcgctt
     2941 tatccagcgc ggcggcacac tctgccgtcg ggcgatccac acgctccatc gtcataccat
     3001 cgtactcaat ggtgttaccg tcacgctcaa cctcgtacag ctcacgtttt acggtaacgt
     3061 tagtgagatc atcagacatc aatgtcaact tgcccggcac ggcaatcacc cgctgccact
     3121 gacggcaatc cagggtatcg ccctctttag tgacaatcag gctgcctatc tcttccggac
     3181 tgaccagcgc acgttgcggc cctttggtct gccagtagcc cgccagccag tccggcgcgg
     3241 gggttttcac gacattgtta tagttttcga cttcggcaca gcccgccagc aacatgagcg
     3301 cacctgcaat tgcgagtttt ttcatcattc gtcctgcatg agaagaaaag aggattgtgg
     3361 cattaaagcc ctgatgtcgc cagcctttcc aggttcagag agaaatttga tctaactatt
     3421 atttttgtga attattaccc cgaaaataaa tttgcatgat gtatgattcg cgattcttgt
     3481 ttcaaacctt ctgagttcag aggctacatt catgtcatgg cagcaattca aacacgcctg
     3541 gttgattaaa ttctgggcac ccatccctgc ggtcatcgcg gcgggtattc tctccactta
     3601 ctattttggc attactggca ccttttgggc tgtcacgggt gaatttaccc gttggggcgg
     3661 tcagctcctg cagctgtttg gcgtccatgc tgaagagtgg ggttatttta aaattatcca
     3721 tctggaagga tcgccattaa cccgcatcga cgggatgatg atcctcggca tgtttggcgg
     3781 ctgctttgcc gcagcgctgt gggccaacaa tgtcaaactg cgcatgccgc gcagccgtat
     3841 ccgcattatg caggccatta ttggcggcat tatcgccggt tttggcgcgc gtctggcaat
     3901 gggctgtaac ctggcggcgt tctttaccgg cattcctcag ttctcgctgc atgcctggtt
     3961 ctttgccatc gccactgcca ttggttcatg gtttggcgcg cgctttaccc tgctgcccat
     4021 cttccgtatt cccgtgaaaa tgcagaaagt ttctgccgcc tcaccgctga cgcaaaaacc
     4081 ggatcaggcg cggc
//
LOCUS       DAZPAC010000304         4066 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZPAC010000304 DAZPAC010000000
VERSION     DAZPAC010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.306"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(139..3654)
                     /locus_tag="ACHUPM_004159"
     CDS             complement(139..3654)
                     /locus_tag="ACHUPM_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR0871782.1"
                     /translation="MDKNALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
BASE COUNT         1059 a         1027 c          840 g         1140 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggt aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataa
//
LOCUS       DAZPAC010000305         4046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZPAC010000305 DAZPAC010000000
VERSION     DAZPAC010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.307"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            31..759
                     /gene="mpaA"
                     /locus_tag="ACHUPM_004160"
     CDS             31..759
                     /gene="mpaA"
                     /locus_tag="ACHUPM_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR0871783.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(734..1699)
                     /gene="ycjG"
                     /locus_tag="ACHUPM_004161"
     CDS             complement(734..1699)
                     /gene="ycjG"
                     /locus_tag="ACHUPM_004161"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR0871784.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1818..2324
                     /gene="tpx"
                     /locus_tag="ACHUPM_004162"
     CDS             1818..2324
                     /gene="tpx"
                     /locus_tag="ACHUPM_004162"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR0871785.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2368..3909)
                     /gene="tyrR"
                     /locus_tag="ACHUPM_004163"
     CDS             complement(2368..3909)
                     /gene="tyrR"
                     /locus_tag="ACHUPM_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR0871786.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          969 a         1143 c          970 g          964 t
ORIGIN      
        1 cctgtaatac ttcctgaacc agagacgatc atgaccgtaa cccgcccacg cgccgaacgc
       61 ggcgcatttc cgcccggaac agaacattac ggacgttcat tattgggcgc gccgttaatc
      121 tggtttcctg cccctgcagc cagccgcgaa agtggtttga ttctggctgg cactcacggt
      181 gatgaaaact cttcggtcgt cacgctctcc tgtgctctgc ggacgttgac accttctttg
      241 cgccgccatc atgtggtgct gtgtgtgaat cctgacggct gccagctggg gttacgggcc
      301 aatgctaatg gtgtggattt aaacagaaac tttccggcgg cgaactggaa ggaaggtgaa
      361 acggtttatc gctggaacag cgccgctgaa gaacgtgatg tcgtattact gacaggtgac
      421 aaacctggct cagaacctga aacccaggcg ctgtgccagc ttatacaccg cattcaacct
      481 gcctgggtgg tctcattcca cgatccactg gcctgtattg aagatcccag acacagcgaa
      541 ttaggcgaat ggctggccca ggcgtttgaa ttgcctctgg taaccagcgt cggttatgaa
      601 acgcctggct ctttcggcag ttggtgtgca gatctcaact tgcattgcat caccgcagag
      661 tttccgccca tctcctccga cgaagccagc gaaaaatatc tctttgcgat ggcaaacttg
      721 ctgcgctggc atcctaaaga tgcaattcgc ccgtcgtgaa ctgaagcgcc ggttccacat
      781 ctaccgccag ccaggtcggt ccgtcaagat cggcgaaact gacctgcggc accagcggta
      841 aagcggcgct aatggcacga gaggtacaca acatgcagcc cagcatcaga ctgaaacctt
      901 gtgcacgcgc ttcagtcgcc agcgccagcg cttccgtcag accaccgatt ttatcgagct
      961 taatgttaac catctcatag cgccctttca gcgccttcag attgctacgt gtatgacaac
     1021 tctcatcagc acaaatcggc aacggatgaa taaaattctc cagcgccgca tcatcctgcg
     1081 caggaagggg ttgttcaagc atcgcaacgc ctaaatccgc caatagctgg caacgcgccg
     1141 ccaccccttc tgcacgccag gattcatttg catcaacgat cagcgtcgca tcgggcacag
     1201 ctgtgcgaat tgccaccatc cgctcactaa taagatggtt atccagcttc actttcagta
     1261 atttcgcgcc tgcctgccag agtgttgatg cactattggc catctgatca ggcgtaccga
     1321 tgactaccgt ctgtgcagtg ataactgtct cgggaagcgt tatgccgatc aaatcagcca
     1381 gcgattgctg ctgttttcgc gcggccagat cccacaatgc acaatccagc gcattacgtg
     1441 ctgcgccggc agggagaatt ttttgcaact cctcccgtgt cagccctttc tctaattgcg
     1501 acacgacgct cataatttgc gccattaccg aggcatcact ttctccataa cgcggatacg
     1561 gcgtgcattc gccggtgcct ttaataccct cttcttccag ttcaaccacc accacgcgcg
     1621 cttcactgcg acttccccgg gcaatcacaa acggggtatg taagggccag gcttcctcaa
     1681 ataccttaac ggttctcatc tttactcctt gttgtcgcgg tggtctgcgg attaagcgca
     1741 aatagcgttt gctgtgttat tgacagttag cataaactag gtgtgacgtt aactatatgt
     1801 aaacaggaaa gataatcatg tcacaaaccg ttcatttcca gggcaacccg gttacagtcg
     1861 ccaattccat cccgcaggcg ggtagcaaag cgcagacttt tactctcgtg gcaaaagatc
     1921 tgtctgacgt caccctcggt cagtttgcgg gtaaacgcaa agtgctgaac attttcccga
     1981 gtattgatac cgatgtttgc gccgcatcag tacgcaagtt taaccaactg gcaactgaga
     2041 tcgacaacac cgttgtgctg tgtatctctg ccgatctgcc gttcgcccag tctcgtttct
     2101 gcggcgcaga aggtctgaac aacgttatca ccctctccac tttccgtaac gctgaatttc
     2161 tgcaagctta cggtgtggca attgctgatg gcccactgaa aggtctggca gcgcgtgccg
     2221 ttgtggttat tgacgaaaat gacaatgtga ttttcagcca gctggtggat gaaatcacca
     2281 ccgagccgga ttacgaagca gctctggctg tactgaaagc ataagttaat atgcctgatg
     2341 gtgttgcacc atcaggcata ttcgcgctta ctcttcgttc ttcttctgac tcagaccata
     2401 ttcccgcaac ttattggcaa tcgcggtatg tgaaacgccg agacgttttg ccagtttgcg
     2461 cgtgctggga taattgcgat aaagctgggt taataccgag cgttcaaaac ggctggtgat
     2521 ttcgtccagc gaaccttcca tcgcatcttc gcccacggct accgttgcgg cgtcataatc
     2581 cggcaacaaa atatcctgtg gacgcagctc ataaccgtcc agttgtgtca gtgcgcgata
     2641 gatagcgttc tttaactgcc gcacatttcc cggccacgca taacgcgtaa gtacagtatt
     2701 caggtcagcg gccagtttcg gacgctgcac gccctgctcg tcggcaaagc gggcgacgaa
     2761 cagctcagtt aacggcatga tgtcctgcgg acagtcacgt agcggcggca gattgagcgt
     2821 caacacgttc agacgataat agagatcttc acggaacatg cctttttgca ccagttcgac
     2881 cagattcttc tgcgtaacgc aaatcacccg cacatcgaca tgcacctcat ggtcttcgcc
     2941 aacccgacgg aaagtgccat cattaaggaa acgcagtaat ttcgcctgca tccgtggtga
     3001 catttcccct atttcatcca acagcaccga accaccgttc gcctgctcaa agaatccttt
     3061 cttcccttcc ggagcatgac caaacagttc actctcgacc gcatcttccg gtatagacgc
     3121 acagttcagc gccaggtaag gtttgcccgc tctggggctt gcctgatggc aggcgtaggc
     3181 aaagagatct ttacctgtac ctgtgtcacc cgtaatcagc agcggcgcgc ttagcatcgc
     3241 cagtttctgc gcctgttcga caacatgctt cattttcggg ctgacggcga caatttgact
     3301 gaaggcgctg acgtcctggg cggcgacatt ttgcaactgg cggcccatac gaatcgttga
     3361 tcgcaacatc accaccgcac cggtcaggac gtgttgatca ttttcatcct gaagataaac
     3421 aggcgtaatc tccatcagga aattctgccc attaataacg acatgctcgt tatgcgaatc
     3481 ttgcggttcg ctttccagcc aacgtaaaaa attaaagccg ttaatcaatt gtgcggcggt
     3541 atggttgcgc aggcgatcca atttttgccc aaaaagctga cagctcgccg ggttcgccat
     3601 atccactttg cttttcatat cgacagagag cacaggttca ggcaacgcct ccagtaacgc
     3661 gctcaacgcc agatgctcac gttcggaagg catccacggg acagtacgca catcggtaac
     3721 acccgcaata cggcgtattt cggccatcag actgctgaaa ctctcaaact ccagttcagc
     3781 aaaattgagg tagattcgcc caatgggatc aatctcaata ccgcgtaaat caatgcctct
     3841 tagcacgagt agatcgagta attcgcgggt cagaccgagt cggtcttcac aaaagacttc
     3901 cagacgcatg ggaaccttca cctgaaaaaa gaacaattaa tatgatgata tgacactatt
     3961 tggatagcag gaaggtttct gtcaacaatc attgacacaa agacggaaaa acgggcactt
     4021 cggcgtaaag atatccggtc agcacc
//
LOCUS       DAZPAC010000306         4010 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZPAC010000306 DAZPAC010000000
VERSION     DAZPAC010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.308"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            124..327
                     /gene="aaeX"
                     /locus_tag="ACHUPM_004164"
     CDS             124..327
                     /gene="aaeX"
                     /locus_tag="ACHUPM_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR0871787.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            335..1267
                     /gene="aaeA"
                     /locus_tag="ACHUPM_004165"
     CDS             335..1267
                     /gene="aaeA"
                     /locus_tag="ACHUPM_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR0871788.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            1273..3240
                     /gene="aaeB"
                     /locus_tag="ACHUPM_004166"
     CDS             1273..3240
                     /gene="aaeB"
                     /locus_tag="ACHUPM_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR0871789.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            3332..3604
                     /locus_tag="ACHUPM_004167"
     CDS             3332..3604
                     /locus_tag="ACHUPM_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR0871790.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(3660..3923)
                     /gene="yhcN"
                     /locus_tag="ACHUPM_004168"
     CDS             complement(3660..3923)
                     /gene="yhcN"
                     /locus_tag="ACHUPM_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR0871791.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT          931 a          981 c         1061 g         1037 t
ORIGIN      
        1 acgctaatat attaaatatc aatctacagc aatgttgctc tcgcccggct ttccatgccc
       61 ttctctgcgg cgacagatgc tgaaaataat aacgcctgct ctctctttac aaccaaggtc
      121 aacatgagtc tgtttcccgt tatcgtggtg tttggactgt ccttcccacc gatatttttt
      181 gaattgcttt tatcactggc gattttctgg ctggtgcgcc gggtacttgt gccaacaggt
      241 atctacgact ttgtctggca tccggcgttg ttcaacaccg cgctctattg ctgcttgttt
      301 tatttgatat cgcgactgtt cgtttgaggt tgaagtgaaa acactaataa gaaaattctc
      361 ccgtacggcc atcacggtcg tattagtcat tctagccttc atcgcaattt ttaatgcctg
      421 ggtctattac accgaatccc cctggacgcg tgacgcgcgc tttagcgctg acgtcgttgc
      481 gatcgcgccg gacgtttctg gactcattac ccaggtgaat gttcatgata accagctggt
      541 gaaaaaagga cagatactgt tcaccatcga ccagccgcgc tatcaaaagg cgcttgagga
      601 agcgcaagcc gatgttgctt attatcaggt actggcacag gagaaacgcc aggaggccgg
      661 acgtcgtaac cgtctcggtg tgcaggcgat gtctcgcgaa gagatcgacc aggccaacaa
      721 cgtactacaa acggttctgc atcagttagc gaaagcgcag gcgacccgcg atctggcaaa
      781 actggatctt gaacgcacgg tgatccgcgc gccagcagat ggctgggtga ccaacctcaa
      841 cgtctatacc ggtgagttta ttactcgagg atcaacggcg gttgcgctgg tgaaacagaa
      901 ctccttctat gtactggcct atatggaaga aactaagctg gaaggggggc gtccggggta
      961 tcgtgcagag atcacgccgc ttggcagtaa caaagtgctg aaagggactg ttgatagtgt
     1021 tgccgcaggg gtcaccaacg ccagcagcac gcgtgacgac aaagggatgg cgactataga
     1081 ctctaatctt gaatgggtgc gtcttgcgca acgtgttccg gttcgtattc gtctcgacaa
     1141 ccagcaagag aacatctggc ctgcgggcac cactgctaca gtggtggtca ctggcaaaca
     1201 agatcgcgac gaaagccagg attcgttctt ccgtaaaatg gcccatcgcc tgcgtgagtt
     1261 tggttaatca cgatgggtat tttctccatt gctaaccaac atattcgctt tgcggtaaaa
     1321 ctggcgaccg ccattgtact ggcgctgttt gttggctttc acttccagct ggaaacgcca
     1381 cgctgggcgg tactgacagc ggcgattgtt gccgccggtc cggcctttgc tgcgggaggt
     1441 gaaccgtatt ctggcgctat tcgctatcgt ggctttttgc gcatcatcgg cacatttatt
     1501 ggctgtattg ccggactggt gatcatcatt gcgatgatcc gcgcaccatt attgatgatt
     1561 ctggtgtgct gtatctgggc cggtttttgt acctggatat cctcgctggt acgaatagaa
     1621 aactcgtacg cgtgggggct ggccggttat accgcgctga tcattgtgat caccattcag
     1681 ccggaaccat tgcttacgcc gcagtttgcc gtcgaacgtt gtagcgagat cgttatcggt
     1741 attgtgtgtg cgattatggc ggatttgctc ttttctccgc gatcgatcaa acaagaagta
     1801 gatcgagagc tggaaagttt gctggtcgcg caatatcaat taatgcaact ctgtatcaag
     1861 catggcgatg gtgaagttgt cgataaagcc tggggcgacc tggtgcgacg caccacggca
     1921 ctacaaggca tgcgcagcaa cctgaatatg gaatcttccc gctgggcgcg ggccaatcga
     1981 cgtttaaaag cgatcaatac gctatcgctg acgctgatta cccaatcctg cgaaacttat
     2041 cttattcaga atacgcgccc ggaattgatc actgatactt tccgcgaatt ttttgacacg
     2101 ccggtagaaa ccgcgcagga cgtccacaag cagctcaaac gcctgcggag agttatcgcc
     2161 tggaccgggg aacgggaaac gcctgtcacc atttatagct gggtcgcggc ggcaacgcgt
     2221 tatcagcttc tcaagcgcgg cgttatcagt aacacaaaaa tcaacgccac cgaagaagag
     2281 atcctgcaag gcgaaccgga agtaaaagta gagtcagccg aacgtcatca tgcaatggtt
     2341 aacttctggc gaaccacact ttcctgcatt ctgggcacgc ttttctggct gtggacgggc
     2401 tggacttccg gcagtggtgc aatggtgatg attgcggtcg tgacgtcact ggcaatgcgt
     2461 ttgccgaatc cacgcatggt ggcgatcgac tttatctatg ggacgctggc cgcgctgccg
     2521 ttagggctgc tctacttttt ggtgattatc cctaataccc aacagagcat gttgctgctg
     2581 tgtattagcc tggcagtgct gggattcttc ctcggtatag aagtacagaa acggcgactg
     2641 ggctcgatgg gggcactggc cagcaccata aatattatcg tgctggataa cccgatgact
     2701 ttccatttca gtcagtttct cgacagcgca ttagggcaaa tcgtcggctg tgtgctcgcg
     2761 ttcaccgtta ttttgctggt gcgggataaa tcgcgcgaca ggactggacg tgtactgctt
     2821 aatcagtttg tttttgccgc tgtttccgcg atgactacca atgtggcacg tcgtaaagag
     2881 aaccacctcc cggcacttta tcagcagctg tttttgctga tgaataagtt cccaggggat
     2941 ttgccgaaat ttcgcctggc gctgacgatg attatcgcgc accagcgcct gcgtgatgcg
     3001 ccgatcccgg ttaacgagga tttatcggcg tttcaccgac aaatgcgccg cacagcagac
     3061 catgtgatat ctgcccgtag cgatgataaa cgtcgtcggt actttggcca gttgctggaa
     3121 gaactggaaa tctaccagga aaagctacgc atctggcaag cgccaccgca ggtgacggaa
     3181 ccggttcatc ggctggcggg gatgctccat aagtatcaac atgcgttgac cgatagttaa
     3241 gtcaaaaccg acgccaaaag cgtcggtttt ttcatggcta tacttagcga tgcacggcag
     3301 aactcgccgc gaaacgtgac ggtggcaaca gatgaatatt tatacctttg attttgatga
     3361 gattgagagt caggaggatt tttatcgtga ctttagccaa acctttggtc tggctaaaga
     3421 taaggtacgc gatctcgact cactatggga tgtgttaatg aacgatgtcc tgccgctacc
     3481 acttgagatt gaatttgttc atctgggaga gaaaacgcgt cgccgttttg gcgcgttaat
     3541 attgctgttt gatgaggcag aggaagagct ggaagggcat ttgcgtttta atgttcgtca
     3601 ttagcgcaaa aaaaagcccc cgaaccgggg gcaatatcgt cggacaagac gatgagggtt
     3661 tatttgtaca gttcagccgt agcgtgccag gtgtcaccgc tacgagcttc agtaatctgg
     3721 taggccgttg cgcctttctc ttccgctttt ttgttcagca tttcacgcat atccattggc
     3781 gaagacgcca caccacttac ggatacggtc ccgattgctt cacgattttg tgcttgtgca
     3841 gtatcaatgg agtcggcagc gaatgcaccg aaagagagaa cagaaagtac gcttaatgca
     3901 gctacagtgg ttttgatttt catgattttt acctcgacat aatcttttag ctgggccttt
     3961 gtttcgtgat ccatatcaca aaatcaagca tacactaatt attgaactaa
//
LOCUS       DAZPAC010000307         4003 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZPAC010000307 DAZPAC010000000
VERSION     DAZPAC010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.309"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..503
                     /locus_tag="ACHUPM_004169"
     CDS             <1..503
                     /locus_tag="ACHUPM_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0871792.1"
                     /translation="HNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNN
                     KILKHIKSHLIMLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTID
                     GLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSY
                     REVNRD"
     gene            496..978
                     /gene="yqeJ"
                     /locus_tag="ACHUPM_004170"
     CDS             496..978
                     /gene="yqeJ"
                     /locus_tag="ACHUPM_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFR0871793.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1012..1092)
                     /locus_tag="ACHUPM_004171"
     CDS             complement(1012..1092)
                     /locus_tag="ACHUPM_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871794.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1128..1552)
                     /gene="yqeK"
                     /locus_tag="ACHUPM_004172"
                     /pseudo
     CDS             complement(1128..1552)
                     /gene="yqeK"
                     /locus_tag="ACHUPM_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            1982..2472
                     /locus_tag="ACHUPM_004173"
                     /pseudo
     CDS             1982..2472
                     /locus_tag="ACHUPM_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2697..3188
                     /locus_tag="ACHUPM_004174"
     CDS             2697..3188
                     /locus_tag="ACHUPM_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0871795.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            3523..3978
                     /gene="ygeH"
                     /locus_tag="ACHUPM_004175"
                     /pseudo
     CDS             3523..3978
                     /gene="ygeH"
                     /locus_tag="ACHUPM_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
BASE COUNT         1495 a          644 c          646 g         1218 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 ttatggcatg caataaaaac tataaccaac atgaagtggc agactgttta accctgtctt
      481 accgtgaggt taatcgtgat tgattataaa aaaaacttat tatttatttt agtttttatc
      541 agtgggttta ttctcttcac tgtctattct tacactgcag aaaaaatgat atataacgaa
      601 acatgcactg caaattgggt aatttttaat gaccagggac gagcaaatct taccatcgac
      661 tttatgtata acaaaaaaaa taaaacagga actgttgcgt tgagtggaac ctggcaacaa
      721 ggtaacagag aaagtaaatc aatacggcga aatattgaat acacatggat tgaaaactat
      781 gacacagccc atttaacatc aaagaaagtt aataagtttg agattatgga tcaagttgat
      841 gatgatagac ttgcacaact tattcctgat ttttatgtct ttccagaaaa aagtgtaagc
      901 tataatatac taaagcaagg taagcatgct tttattttga gcattggtaa cagagcaata
      961 atgcattgtg caaggtaaac taaaatagat aacaaaaaag atggaacctc gttaatcata
     1021 aaacaataaa attaaatatt ctcgcagtat atggcagtct aaagcatcaa agttttgatc
     1081 aacatctttc attttagaca tctccttgca atgtaaaata tcatgtatca acaacatctg
     1141 gtgcaaatcc atgagtcgaa ctcgacattc atcttagctc gattattatt ccttcgtaca
     1201 gtcgatgtaa acaatacaga aagaggatta ttaagaacca gtttactcag gaatcaatag
     1261 aggaaaggac taacgtttct ttaaaagaat tgatttcatc atctgttaaa ctaaactcat
     1321 cattgacaga tcgtgagata taactgtttt taactttact ctttacgttg actttattga
     1381 cagaattaac attcacatat cttgaattta atgtccatga tgttgtttgc gtgagaacat
     1441 tctcagcatt aaggaatttt tttaacggaa tccttggttt ctttttcgag tccgaattta
     1501 caatatcatg catatagacc atttcatgaa tctgcgaaaa ctcaatgtcc attgtatacc
     1561 tcacattttt accgtgactc gatgttactg ttcaataatc accttccatc aatactaaaa
     1621 ttaatacccc taatgtgccg ataacaaata tagtcattct acgtaacgtc tccataaggt
     1681 gataaccttg atcaaatgaa tttcgaatat aataaaggaa atcttttgag atgtttatca
     1741 cgaataaagc tatttatgtt accaatatca tttcgagtgc gcacactatc tatctgtttt
     1801 ttcgagatat aaaaaacaac gtgtaagtta tgtaaattgc cgatgctact gagtaacatt
     1861 atggtaaaaa aagtaaaaat gagcgatttt tgatagaata aagcatgaga aaaggtgata
     1921 tttgacatta tcagaagctg cgaattcgga ttttgctcta atcaaatgag agaaatgtaa
     1981 aatgaagcca cgaaatatta ataatagcct accactgcaa ccattagttc ctgatcagga
     2041 gaacaaaaat aagaaaaatg aagagaaatc cgttaatcca gttaaaatca caatggggtc
     2101 tggtttaaat tatattgaac aagaatctct tggaggaaaa tatctaacac atgatttgtc
     2161 aataaagata gcggatattt ctgaagagat aattcagcaa gcaatattat ctgctatgag
     2221 catatataaa ttttcgataa cagatgattt aatgagtatg gctgtaaatg aactcataaa
     2281 actgaccaaa atagagaata atgtagacct gaataaattc actactatat gcacagacgt
     2341 tctatccccc ctcgtcacca gacataataa agaaaaaaaa caaacgacat tctacccttc
     2401 tcaaaaatcc cctttttaat tttcattgaa aaaatggcaa ttgatactat gaatccaggt
     2461 catacacatt aaggcattta tgtaatgtcg taattaagat aactaataag gtgaatatta
     2521 gtaaaacaac taatgatgtt attagtttgt agtgaacagt acttttacca ataatgaaaa
     2581 atataccata agcaatgcaa gcttaaaact aataatttaa aaaagataaa tgtaaaaata
     2641 ttaatgagtc aatcaataat aatttagcat cacgagacac atcacagagg aatattatga
     2701 gcacagaaac aattgaaata ttcaataata gtgatgaatg ggcaaatcaa ctaaaacacg
     2761 cattatcgaa aggagaaaat ctggcattac tacatggttt aactcctgat atccttgata
     2821 gaatatatgc atatgcattc gactaccatg aaaagggtaa tataacggac gcagaaattt
     2881 attataaatt tttgtgcatt tatgcgttcg aaaatcatga gtatctaaaa gattttgcat
     2941 cagtatgtca gccaaaaaag aaatatcaac aagcatatga cctttacaaa ctaagttaca
     3001 attactcccc gtatgatgac tattcagtta tttatcgtat gggtcaatgt cagattgggg
     3061 ctaaaaatat cgataacgca atgcaatgtt tctatcacat tattaacaat tgtgaggatg
     3121 atagtgttaa gagtaaagcg caggcatata ttgaactctt aaacgataat tcagaagata
     3181 atggctaagc attacaatca tttggttaaa tttgctggtt atagtgcagt taactactat
     3241 aaccagcacc tcttatgttt tcttaaatca gcatcttaaa acagttaaga gcatactata
     3301 aatcattatt tttcaatgcg ttagagaaca atctacttta taaaaattga ataaggctat
     3361 ttcagattac atcactgaat tcctaagata aattagtgaa cacaaccata gatatgtcga
     3421 atgtattttc ctgtagcatt aatgacacat aaatatgaat agccataatt tctattgctt
     3481 aagcaggatg caagaaacca attttttcat agaggttaac taatggactt agaaaataaa
     3541 ttctcatatc attttcttga gggattaacg ctcacggaag atggaattct tactcaagga
     3601 aatgagcaag tttatattcc acagaaagag ttaggtgtat taatagtatt acttgaatcc
     3661 gctggtcatg ttgtactgaa agatatgatc atcgaatcag tatggaaaaa tattattgtt
     3721 agtgacgagt ccctgacaag atgtatctat tctttgcgct gcatttttga aaaaattggc
     3781 tatgatcgtt gcatagaaac aatctaccgg aaaggttatc gtttcagcgg gcaggttttc
     3841 aaaactaaaa ttaatgaaga taatacttca gactattcca tagctatatt ccctttcact
     3901 acttcattga atacactgga tccattaata cttaatcagg aattagtgca aatcatttca
     3961 aataaaaaaa tcgatggtta gactggcccc ctgaatctcc aga
//
LOCUS       DAZPAC010000308         3989 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZPAC010000308 DAZPAC010000000
VERSION     DAZPAC010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.310"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..1898
                     /gene="tssM"
                     /locus_tag="ACHUPM_004176"
                     /pseudo
     CDS             1..1898
                     /gene="tssM"
                     /locus_tag="ACHUPM_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1909..3358
                     /locus_tag="ACHUPM_004177"
                     /pseudo
     CDS             1909..3358
                     /locus_tag="ACHUPM_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            3285..3767
                     /locus_tag="ACHUPM_004178"
     CDS             3285..3767
                     /locus_tag="ACHUPM_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR0871796.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
BASE COUNT         1071 a         1028 c         1067 g          823 t
ORIGIN      
        1 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
       61 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
      121 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
      181 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
      241 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
      301 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
      361 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
      421 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
      481 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
      541 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
      601 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
      661 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
      721 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
      781 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
      841 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
      901 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
      961 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
     1021 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
     1081 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
     1141 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
     1201 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
     1261 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
     1321 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
     1381 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
     1441 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
     1501 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
     1561 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
     1621 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
     1681 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
     1741 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
     1801 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
     1861 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
     1921 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
     1981 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
     2041 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
     2101 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
     2161 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
     2221 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
     2281 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
     2341 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
     2401 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
     2461 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
     2521 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
     2581 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
     2641 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
     2701 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
     2761 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
     2821 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
     2881 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
     2941 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
     3001 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
     3061 caggcggagc ggggactggg gcaggaaacc cccattgagt ctctgttgac gcagtaccat
     3121 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
     3181 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
     3241 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
     3301 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
     3361 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
     3421 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
     3481 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
     3541 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
     3601 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
     3661 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
     3721 tagccggtac gaatgcaatg agtaaatggc aggatcgtgt acagtaactt actgtaataa
     3781 cataataaat tactattgcg gagagataaa atggctggtt taaggaataa caacaacaca
     3841 cagaatgcac agtgggctga ttatgtgggt gacattcttc ggggggctca gccgataaac
     3901 cagctagtac ctcagcaccc ttatcttaac gacgtcccac tcattgatga gttaaggcat
     3961 cagaacactc atcatgttat tctcttgac
//
LOCUS       DAZPAC010000309         3975 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZPAC010000309 DAZPAC010000000
VERSION     DAZPAC010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.311"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..1361)
                     /locus_tag="ACHUPM_004179"
     CDS             complement(<1..1361)
                     /locus_tag="ACHUPM_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR0871797.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1426..1545)
                     /locus_tag="ACHUPM_004180"
     CDS             complement(1426..1545)
                     /locus_tag="ACHUPM_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR0871798.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1769..1927)
                     /locus_tag="ACHUPM_004181"
                     /pseudo
     CDS             complement(1769..1927)
                     /locus_tag="ACHUPM_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2272..3750)
                     /locus_tag="ACHUPM_004182"
     CDS             complement(2272..3750)
                     /locus_tag="ACHUPM_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFR0871799.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKVESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWRTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1120 a          873 c          688 g         1294 t
ORIGIN      
        1 actaaagcac tgcccgtctc ctggttaacc ccatgtgcta taccgccggt atagacaatc
       61 agcgagcctc cggcgctaat attgctgcca gttgccgtac catctttctc aacaacctgc
      121 cggctcccgg tggaaatatt tgtcgtgtca gctttcccgc cgctcttgat attttgtgtt
      181 ccgctgttga tattggtacc cgtggcaata ccatgattat taatattctg tgtaccacca
      241 ttgattatgg tattggtcgc gtttctggta acatccatta ccccgccgtt atctattcgg
      301 gtcgcatcag ctttagcatt cgttaaaact gacattgttc ctttatcttt aataatcgtc
      361 ttatttgccg aaccatatgc gtttatgtct aaatgaccac cgttttccag caacacattg
      421 tctgccacgt gattgtggat ggagaatgca ccttcactat tcgtaccgct caccgtcgta
      481 ccgttagtgt ttgtttttaa aattgcacca tcgtgctggg taacatttgt tgccgtacca
      541 ccactaacat caagcacgcc accggaataa acttcaataa catccgagga gctggtgtaa
      601 tcaacaattt gcgtaccacc agaatagatc tgagtatttt ttgccgttga cttattattc
      661 agagactgag ttccaccttc aatcgtcgtg tccagcgcat ggctttcata tactctttgc
      721 tcaccgccat ttttaatggt ggttgttcct actgtgctct gttcaacata ctgtcgtcca
      781 ccatttatga ttgtgttcgt tgccatactt ccttgcgtga tgtcctgata accggactta
      841 tttatcgttg taccatcaac atgcccctga gttgttactg tctgccctcc accatcaatg
      901 atggttccat tcgcagtccc cccacttatg tgtgaattac cacccttaat tgtcgttcca
      961 ttgctgatac cgcctgtata aacgtcctga ttgccactgt cgattatcgt accggtggaa
     1021 atgcccccgt catgaattgt ctgtcttcct ccgttaatgg ttgtattatt agactgcccc
     1081 atataattgt tattccttcc aacatcttga tatccaccag attcaataag acttccatta
     1141 gatactccgc cataaacact ctgctggcca tggttgataa tatgagtgtt atttgttgta
     1201 cctcgttcat ctactcgttg gttgccatct acagtctcat cgtttaccac accaataaca
     1261 ttagtagtaa aggccgccat cacgggcggg gcatatatca aggcagatat caataaggaa
     1321 agtactgagc ggcgacaata atggggactg gtcctgttca taaatttcat cctctgaaaa
     1381 gtgaatactg agtagcgttt aagcgacttt agctctgctg caacatcagc ccgcaggcac
     1441 cagaccgggg ggttcatcct gaagagacag cgcaagtgta ttgtgttcac cgctcatcaa
     1501 agacatcatg atgaaatgat gatgttccac ataagaaaga ggcatttttt aaacacagtg
     1561 cgctgaagtg tgattggata aaaaagtcaa tctattcagg aaatacgggc gtattctttt
     1621 ctttcgacaa tcaggccgtc ggcaaaataa aatgatttac ataatcgttt ctgatgaata
     1681 tcttctgctc acataaaaat cacacaataa ctttgagatc gcagattctt ttacttttac
     1741 agcattcgtc cccccattgt tgggcaaata tagattgggc cagagcacga aagttaatac
     1801 cacgtttgca cagctcctcc aacagcacga caagatgcca catactgcgc cccagtcggt
     1861 gcggtttaca gactacccgt gcgtccaccg ccgataatgt cctgagcagt tttttcagtc
     1921 cggacctcct gccacggttt tacgttgaag aggtaaccct gagcacgcag ttcttcagtc
     1981 aggcgtggtg caccgtaacg ctgttattgc tgggtaagat caaaaaactt tcaggcagct
     2041 aaggaaagtt gaaccagaca ttagatgaaa tatttcaacc aattacagca ccaatttaaa
     2101 ctcagtaaat cacagcacag atcaaggggt tacgtgaaag cgtagcccct ttttctttgg
     2161 tagtggcagc aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca
     2221 tattgtttca atctacgtta ttatctcttt gtaaaaatag ccatttatta atcattgaaa
     2281 actgctttta gaacttgata caacgggact agtcacaaca ggactattct caacgggatc
     2341 atcctcagag gaactatcat caaagtcatc atccataaat aaaatatcat cgaatggtgc
     2401 cacgcccgtg atgagtttta ttttattatt acgatcagtc aagactccac ttaaaccgtt
     2461 ttcgctcaca ggttttaatg atttttcatt actcttgttg taagcaggcg cattaaaaat
     2521 acacggagta tcaagatcaa acaatgacgt tctccagttc acatattgaa tatcatagtt
     2581 actgaagttc tgtccagaaa agaagcatcc cttaaaatcc aatccaccta aattatataa
     2641 accatcctct tctttttgga gagtaatgtt aattctggct atctcccgga caaaacttcc
     2701 ttttttgtat ttgaataccg tttcaagatt ttcaccagac agcttgactt ctgggaataa
     2761 tttaaaatta aaccctacat cattattatt caaagtagat gaacgaaaga tggaaaaagc
     2821 ttgtagtgct gaattatagc tttcgacttt atcttgaggc tgcgctcttg gtaaaaactt
     2881 atagcagcac tcataaaaat tgaataaacg ttctgaacaa ttcccatttt tatcaattaa
     2941 taccccccgg aattctttca caaaggcatc cgttattttt tgaatatcta tggtgtgttc
     3001 ctttgactct gacaaagatg gagtctgttg aagtgcattg ctcatctggt taaggaacat
     3061 ctgcatttct ttaaatttat ctccatagtg gcctttaact tgctgagagt ttttagttac
     3121 aggtgaaacc ttatctattt ggttgtcata taaattatgt ggttccatac agacatttgg
     3181 ttgtgtttct aaaataacac tacgtgtact acctggctta aatgaatcaa catgaaaatc
     3241 actttttctt atattatcga atagggtctg ttcatttctc ttagcgcatt caataaactg
     3301 atcaacatta tttttattcg atgacaggtc agtttcagaa accgcatttt ctttgttctc
     3361 cagctttagt ttaatgagaa gatcttccca gacctgcttg cttaaacata ctacgtcagg
     3421 ctcaccatca gtaactaact cttcattatt atcaaagtat acgttgaata cttttaagtt
     3481 attttcatca acatcatatt taatacgttt taattgctct ccagatccca taatgacaaa
     3541 ggcgttgcct tcatgatata tacattcaga catcattttt tgtaaagttt caagagcacc
     3601 gcgatacgtc cctgaggcag cttccttaca aattaaattt ataatactat aagctagtag
     3661 ctccatggat tcagaagatt ttctttcatt acaatttcca ctatctataa atgagctagt
     3721 gttaaattcg ttgcttttac taactaacat tgtctattcc tcagttaatg tctacatggc
     3781 tatttttaat tttattattg tttgtcacta taaaaaatcg ctcatttgag acaattgctg
     3841 gcattaacag cttcatatgc tatacatggt acttttaatt aaattagcac aagaatgtta
     3901 aatttaataa acaaaaagtt atttcgctgt atgataaaaa ccacccgtta taatttatta
     3961 gtgaaattcg ttttt
//
LOCUS       DAZPAC010000310         3973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZPAC010000310 DAZPAC010000000
VERSION     DAZPAC010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.312"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(263..463)
                     /locus_tag="ACHUPM_004183"
     CDS             complement(263..463)
                     /locus_tag="ACHUPM_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871800.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(463..735)
                     /locus_tag="ACHUPM_004184"
     CDS             complement(463..735)
                     /locus_tag="ACHUPM_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR0871801.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(738..1016)
                     /locus_tag="ACHUPM_004185"
     CDS             complement(738..1016)
                     /locus_tag="ACHUPM_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871802.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(1017..1574)
                     /locus_tag="ACHUPM_004186"
     CDS             complement(1017..1574)
                     /locus_tag="ACHUPM_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871803.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
     gene            complement(3138..3689)
                     /locus_tag="ACHUPM_004187"
     CDS             complement(3138..3689)
                     /locus_tag="ACHUPM_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0871804.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
BASE COUNT         1109 a          822 c          738 g         1304 t
ORIGIN      
        1 aattgataca ccggaatcag cgaaagcctg caccatcata gcagtgtcgc tatcaatgaa
       61 aatctgtaat tcatattcgt tcatttttaa aatctccttt ttaccagacc tcctcaatcg
      121 ctctgtaacg agctaaacag tctttttcag gggtagcctg tacagtggta ttacccaccc
      181 taaaataaga tagttttttg cgttagaatg cgattgagag cgtctgataa gctgtttttc
      241 taatttctct taaagtctcg gactaccaat aacctaattc cgctttattt cgttcaaacc
      301 attccgctgt aactgctgtc tttagtgttg cgtttgggct ggtggcatga atgaagatat
      361 cttttggatt gcaagcatca ccgaaaccac cttctttgat gagcaggtct gatacatgct
      421 taatcatttt atctgtcaaa tattcgaatt taatacataa cattaaatct ccaggagtaa
      481 attgtttggg tggcaatttc ttttattacc atccctgaag ataatctttt tagttgcctg
      541 aacttcatga tgcaaaaacc agatgacata cttcagataa taatcacgag tgccataacg
      601 aatcattgga ttaccgtaag catcatctct tgtcttaacc tctccaccat caatccattc
      661 aagcattcct gtctcataat taatcatgaa gtaagttgca aagaaccgtt taatatcatc
      721 tcgccattgc ttcataatca gacctcctga gacagtttgt gaattttgat acttagtaac
      781 cttgctaaat ccagtccaaa agcatttaag gatttcagac tatcaaggtc atcttgctta
      841 atcctgatat ggtgtcttgc agcctctaag cgactattaa gcaaatcctg ttcgttaata
      901 tcacggttaa ttgcaagctt catcactgct gagtgtggca gacctgtaat gtttttgatt
      961 cggttcagtt catcaaaggc tgtctttgta actctggtta atgtgtgttt attcatttaa
     1021 aaatcctctg taactaaata ggggtcaaac tcgaaatctg gttgctcgtt ttccattggc
     1081 tcaatattcc caacggcaat atgatgttca acaatgccaa ttaatttcgc gcacatcaca
     1141 gcaagcaaag cgcaatattt atcgcttggt aattcgtaac taagtctggt tgcctgtcgc
     1201 tgaagatgat ttgtcacggt ggatgcgtta atttcgtcaa gattctttag cagataaaat
     1261 agttttttgt tgtatttcgg gtggccttca cttctacaga acccaataaa aacatcatca
     1321 agcttgcttg gtaactgctc gtaactgtca aagttagcca gataattttt aatagtattc
     1381 tttgttgttt tgcgtactgg cataaatctg tctcgtggcg taatgctacg aatgaccgct
     1441 tcaatgttct tacgttcgga tttgctaaag gttgggactt cgtagctggt ggggttgttt
     1501 gtgtaagcgt aggagttgtg cttgttgcgt tctcttgatg cctgaagcat cgctctaaat
     1561 tctgaatact gcattcatgt tctgactctc ataagatgtt attattctgt taacctatat
     1621 acaatattag cattttcaaa gactaatgtc aagtattttt aacattttcg taacatctgt
     1681 ggtataatgg gtttatgggg agagtaaaat atgaggcgtg gctatgtatt tggtcgcata
     1741 ttttgcattg cacacagacc tcttcaatcg ccctgtaacg agttaaacga cctcttcagg
     1801 tcaactcata aggtcaggta ctttttgtgt cagaatgcga ttgagagcgt cttacaagga
     1861 aaactaatgg taaatctccc tcctgagctg gggtcacctg atgtatggac tggtggaggt
     1921 attgggctga ttacaggcct gctacttcgc tacgtcctcg tcagagtgct tgttgtcttt
     1981 aaactgttcg gtcgtacatg gacgcttgac cttaaggata acgaccacac agacccccgt
     2041 taaactcctt caaaacccct gcacgagatg acagatagaa gtctatggta caggggtgtc
     2101 tttagcattt tgtgctatct attaaaaaaa tagcttgaca gaaccagaaa gccgtatcta
     2161 tcaagcattc acctctctga cagtaatttc taaaagttct tatttttcaa taagttgtaa
     2221 ttagccatat atagagaact aaaagatttt ttagctattt tgtttaaaag catatcaaac
     2281 gatacatttt attgatttag gtcaaagaca aaagcattga caaaatggta agattgttca
     2341 catgacctta cttaacagac ttttaagaga ctttgaaaca gcctgtttaa caggtcacta
     2401 cctctggtct tatcgttccc ttctttctag gtggttatct gttaagagtt tttcagtttt
     2461 tattcttaag ataattgtta agatgatact ctcccttcgg gttatatcgt atctcttctc
     2521 ccgttaaata tcccttcaca gtcagactaa ataacacctg tgtagttctt ggtagaatct
     2581 ttcaaggtaa cttagctctg agtttatgaa gttgacctat gagatagact ctaccgaatc
     2641 tgttagtcaa ttgttgctta ggtgctgcta aggcttactt agtggaaaga gaggggatat
     2701 gtgaccatag gggaacccct cctgttaaaa gactacttag aagtctgagg ttttagtaaa
     2761 atctgtgaat atggcttctc agacgctctg taacgcattc taagcgattt taccccttta
     2821 ttttaaggtg gttagtacat ccctataggt tacccctaaa aatggggtta aaactcgcta
     2881 cagagctttc tatgagatta gcttctaatc gaatcaacta tgaggtttgg taggattgtg
     2941 caaaatctgt acagtaactt ttcagaatct gaaaattttt ataaaaaatg actagccctc
     3001 tttgaatctc atcttccccg aatcttcaaa aatatctgac agatgtctgt agctgctaag
     3061 tacccgtcac accagctatg tgaccccctt ggcaaaaaag ccaaatatca gctacatcca
     3121 tgtaactatt cagtaactta tttagcaagt ctgtaataag ttgctctaga gattcctaac
     3181 tcttcctgaa tctgcaacct cgtcttacct tgtgaaataa gcgtctgtac agcctctgaa
     3241 gaagctttta caggcctccc catatgtacc ccttcagctt tagcacgttc aataccagcc
     3301 tttctacgct ctgctatcaa gcttctttca agctgtgcca acgttgacat aatgcctaac
     3361 attgcttcac ccatagcgct tgataagtca aacccttctc taaggctgat aactgtcaca
     3421 cctttagcct gtagcgcttt aaccgttgat aacacatcaa ttgtgtcacg tccaagacgg
     3481 tctacagcta ccacaactac ggtgtcacct tctcgtgcat aagctaatag tctgctgaaa
     3541 ccgtttctta aagaagcttt gacagaacca ctgacagctt catcagtgaa ccatttagca
     3601 atgttgtacc ctgcctcttc aatttgcatt tgctggttag tgatggattg gtcatctgta
     3661 ctcaccctgc aatatccaag tatcgccata atgttcccct tagaagtctc aaaagttagt
     3721 ctcataagtt tagctgtctc ataatccaaa aacaaccttt tgagactact tgagggtgca
     3781 ttctaccgtt acgttgaaag tgtctcaaaa ggaagtcctt ttaagactgg ttatgaagga
     3841 gctatgaggt tgattagggg ttggtacaag cccaactaac cgaatcaact atgaagattt
     3901 taggagggta actgtaacca tcactttcta actttcacgt aggaaattgt taagtggttt
     3961 gatagttaca cca
//
LOCUS       DAZPAC010000311         3950 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZPAC010000311 DAZPAC010000000
VERSION     DAZPAC010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.313"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHUPM_004188"
     CDS             complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHUPM_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFR0871805.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /gene="sutR"
                     /locus_tag="ACHUPM_004189"
     CDS             1006..1542
                     /gene="sutR"
                     /locus_tag="ACHUPM_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFR0871806.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHUPM_004190"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHUPM_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFR0871807.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="ACHUPM_004191"
     CDS             complement(3668..3898)
                     /locus_tag="ACHUPM_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFR0871808.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1023 a         1008 c         1001 g          918 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ccaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ccagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag
//
LOCUS       DAZPAC010000312         3909 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZPAC010000312 DAZPAC010000000
VERSION     DAZPAC010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.314"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            24..590
                     /locus_tag="ACHUPM_004192"
     CDS             24..590
                     /locus_tag="ACHUPM_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR0871809.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="ACHUPM_004193"
     CDS             594..1169
                     /locus_tag="ACHUPM_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR0871810.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="ACHUPM_004194"
     CDS             complement(1211..2263)
                     /locus_tag="ACHUPM_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR0871811.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHUPM_004195"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHUPM_004195"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR0871812.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3765
                     /locus_tag="ACHUPM_004196"
                     /pseudo
     CDS             3580..3765
                     /locus_tag="ACHUPM_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
BASE COUNT          971 a          987 c          951 g         1000 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta ttcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctgat
//
LOCUS       DAZPAC010000313         3901 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZPAC010000313 DAZPAC010000000
VERSION     DAZPAC010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.315"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHUPM_004197"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHUPM_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR0871813.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHUPM_004198"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHUPM_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR0871814.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHUPM_004199"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHUPM_004199"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR0871815.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="ACHUPM_004200"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="ACHUPM_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR0871816.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
BASE COUNT          937 a          917 c         1018 g         1029 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 c
//
LOCUS       DAZPAC010000314         3864 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZPAC010000314 DAZPAC010000000
VERSION     DAZPAC010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.316"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..1803
                     /gene="fimD"
                     /locus_tag="ACHUPM_004201"
                     /pseudo
     CDS             1..1803
                     /gene="fimD"
                     /locus_tag="ACHUPM_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1816..2823
                     /gene="fimH"
                     /locus_tag="ACHUPM_004202"
     CDS             1816..2823
                     /gene="fimH"
                     /locus_tag="ACHUPM_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR0871817.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2834..3349
                     /gene="sfmF"
                     /locus_tag="ACHUPM_004203"
     CDS             2834..3349
                     /gene="sfmF"
                     /locus_tag="ACHUPM_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFR0871818.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="ACHUPM_004204"
     CDS             complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="ACHUPM_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFR0871819.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL
                     IDYAKLYELI"
BASE COUNT          988 a          845 c          959 g         1072 t
ORIGIN      
        1 tggtgtacgg ctttattctt ccgataatat gtatccagat agccagcaag ggtttgcccc
       61 aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat
      121 tatctatcaa agctatgttt cccccggtgc ttttgaaatt actgatttgc acccgacatc
      181 ttcaaatggc gatctggatg tcaccatcga cgagcgcgat ggcagaatta cacaattccg
      241 tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct gacggcgggc
      301 gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg cacggcactc
      361 ggtggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc caattacacc
      421 gcctttttat tagggctggg gcgcaacctc gggaactggg gcgcagtgtc gctggatgtg
      481 acccatgcgc gcagccagtt agccgacgac agtcgtcatg agggggattc cattcgcttc
      541 ctctatgcga aatcgatgta caccttcggc accaattttc agttaatggg ttaccgctat
      601 tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga ggggtacgaa
      661 tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa ttaccacaat
      721 ttacgcttta gccgcaaaga ccgtttgcag ttaaatattt cacaatcact taatgacttt
      781 ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga ttcagatacc
      841 tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact ctcattttcg
      901 tggaatgaat ctgtagggat ccccgataac gaacatattg tcggacttaa tgtttcagtg
      961 cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga ccgcgcttat
     1021 gcctccttga acgctaaccg taacagcaac gggcaaaata gctggctggc aggcgttggt
     1081 gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggcga tacctcgaat
     1141 aatgggtaca cgggcagcgc cacggcaaac tggcaggcca cttacggtac gctgggggtc
     1201 gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg cggtgtggtc
     1261 gggcatgaaa atggcataac actgagccag cctttagggg ataccaatgt tttgattaaa
     1321 gcgcttggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac cgactggcgc
     1381 ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc gcttgatacc
     1441 aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt gccgacgcaa
     1501 ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct cattaccgtt
     1561 acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag taccggaata
     1621 accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt gtctggtgaa
     1681 ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta tgtattgcca
     1741 aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca tcctcgctca
     1801 taaaggaaat tatcaatgaa gataatctgc agattattat tggcaatggc atgtttgtgt
     1861 ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc agaagatgaa
     1921 cactatgatc tctcaaatgt ctttaatagc accaataacc agccagggca gattgttgtt
     1981 ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt gtccactcgg cacgctggtg
     2041 aattatacat accgtagtta tgtcactaac tttattgttc aggaaactat cgataattat
     2101 aaatatatgc aattaaatga ttatctatta ggtgcgatga gtctggttga tagtgtgatg
     2161 gatattcagt tccccccgca aaattatatt cggatgggaa cagatcctaa cgtttcgcaa
     2221 aaccttccgt tcggggtgat ggattctcgt ttgatatttc gtttaaaggt tattcgtccc
     2281 tttattaaca tggtggagat ccccagacag gtgatgttta ccgtgtatgt gacatcaacg
     2341 ccttacgatc cgttggttac acctgtttat accattagtt ttggtggccg ggttgaagtg
     2401 ccgcaaaact gcgaattaaa tgccgggcag attgttgaat ttgattttgg tgatatcggc
     2461 gcatcgttat ttagtgcggc agggccgggg aatcgaccag ctggcgtcat gccgcaaacc
     2521 aagagcattg cgatcaaatg tacgaatgtt gctgcgcagg cttatttaac aatgcgtctg
     2581 gaagccagtg ccgtttctgg tcaggcgatg gtgtcggaca atcaggattt aggttttatt
     2641 gtcgccgatc agaacgatac gccgatcacg cctaacgatc tcaatagcgt tattcctttc
     2701 cgtctggatg cagctgcggc agccaatgtc acacttcgcg cctggcctat cagtattacc
     2761 ggtcaaaaac cgaccgaagg gccgtttagc gcgctggggt atttacgcgt cgattatcaa
     2821 tgaggtacgg agaatgagaa gagtactctt tagctgtttc tgcgggttac tgtggagttc
     2881 cagtggatgg gcagctgacc ctttgggaac gattaatatc aatttgcacg gtaacgttgt
     2941 tgatttctcc tgtaccgtaa acactgcgga tattgataag acggtagatt taggcagatg
     3001 gcctacgaca caattactga acgctggcga taccacggca ctcgtccctt ttagcctgcg
     3061 gctggaggga tgtcctccgg gttccgttgc gattttattt acgggaacgc cggcatccga
     3121 taccaacctg ctggctctgg atgatcccgc aatggcacaa accgtcgcca tcgaattacg
     3181 taatagcgat cgctcccggc ttgcactggg ggaggcgagc ccgactgagg aagtagatgc
     3241 aaatggcaat gtcacactaa acttttttgc caattatcga gcgttagcca gcggtgttca
     3301 gccaggtgtg gagaaagcgg atgcgatatt tatgatcaat tataattaat attatattaa
     3361 ttcgtataat ttggcgtagt cgataagctc tacaattgaa tgcaaaccta gtttgccata
     3421 aatattagat ttatgcgcac taactgtttt attgctaagt aataacttat cggcaatttc
     3481 tttattagat aatccgctaa ccagataacg taatatggtc acttcacgat tagatagcac
     3541 agtgaccgtt gaactattcg tactacattt attgcttttt atatagttga gcgtttcgct
     3601 aggaaaaaac gtgtatccgg agaggatcat ctgaacggga tgaaaaatat cattctgatc
     3661 attgcattta ctgacaaaac cgttagcacc agcttgtatc gctctgccag cataaaagca
     3721 ttctgatttc gatgataaaa ataacacttt cactgtgctc tggatttgtt tgatcctttt
     3781 caggaaggta aaaccgtctg ttccgggcaa gtctatatcc ataatgatta aatcaacagg
     3841 acgggttcgg agataatcga tggt
//
LOCUS       DAZPAC010000315         3858 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZPAC010000315 DAZPAC010000000
VERSION     DAZPAC010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.317"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            77..2356
                     /gene="ydeP"
                     /locus_tag="ACHUPM_004205"
     CDS             77..2356
                     /gene="ydeP"
                     /locus_tag="ACHUPM_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR0871820.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2604..2801
                     /gene="safA"
                     /locus_tag="ACHUPM_004206"
     CDS             2604..2801
                     /gene="safA"
                     /locus_tag="ACHUPM_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR0871821.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            2876..3637
                     /gene="ydeO"
                     /locus_tag="ACHUPM_004207"
     CDS             2876..3637
                     /gene="ydeO"
                     /locus_tag="ACHUPM_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR0871822.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
BASE COUNT         1085 a          845 c          879 g         1049 t
ORIGIN      
        1 cgataatccc tttgtatgta ctgtgcatca tcgctattac aaatcctaat aattcatttc
       61 cacacaggat aagtagatga agaaaaaaat tgaatcctac cagggtgctg caggtggttg
      121 gggtgctgtt aaatccgtag cgaatgcagt acgtaagcag atggatatac gccatgatgt
      181 tattgccatg tttgacatga ataagccaga gggctttgac tgtccgggtt gtgcatggcc
      241 agatcctaag cacagtgcgt cattcgacat ttgtgaaaac ggcgcaaaag caatcgcctg
      301 ggaagtcacg gataagcagg taaatgcctc tttctttgct gagaatacgg ttcaatcatt
      361 acttacctgg ggagatcacg agcttgaggc tgcggggcgt ctcactcagc ctttgaaata
      421 tgatgccgtc agcgactgtt acaagccatt aagctggcaa caagctttcg acgaaattgg
      481 cgcacgcctt caaagctata gtgatcccaa tcaggttgaa ttctatactt cgggccgcac
      541 ttccaatgaa gctgcctttc tttatcagct ttttgcccgt gaatacggga gcaataactt
      601 tcccgactgc tccaacatgt gccatgaacc gacaagcgtg ggtttggcag cgagtatcgg
      661 tgtaggtaaa gggaccgtgt tgctggaaga ctttgagaag tgcgatttag tcatttgcat
      721 tgggcataac cctggtacaa accaccctcg catgctgact tcgttgcgcg ctttagtgaa
      781 acggggagcg aaaatgatcg ccatcaatcc tctacaggaa cgtggcctgg agcgatttac
      841 cgcaccgcaa aacccgtttg aaatgctgac gaactctgag actcagttgg ccagtgccta
      901 ctacaacgtg cgcattggtg gcgatatggc gttgctcaag gggatgatgc gcctgttaat
      961 tgagcgcgat gatgctgcaa gtgccgcagg tcggccctca ttgcttgatg acgaatttat
     1021 tcaaacgcat accgtcggct ttgacgagct acgccgtgac gttctcaatt ccgagtggaa
     1081 agatatcgaa cgtatttctg gactaagtca gacacaaatc gccgaactgg ctgacgcata
     1141 tgccgctgcc gatcgcacca ttatctgtta cggaatgggg atcactcagc acgaacatgg
     1201 tacccagaat gtacagcaac tggtcaatct gctgttgatg aaaggtaaca ttggcaagcc
     1261 tggtgcgggt atctgcccac tacgaggtca ctctaatgta cagggcgacc gaaccgtcgg
     1321 tatcaccgag aaaccgtctg cagagtttct ggctcgcctg ggtgagcgct atggcttcac
     1381 cccacctcat gcacctggac atgctgcaat tgccagcatg caagcaatat gcacggggca
     1441 ggctcgagca ttgatctgca tggggggcaa ctttgcactg gcaatgccag atcgggaagc
     1501 gagcgctgta ccgttaacgc aattagattt ggcggtacac gtagccacta agcttaaccg
     1561 ctctcatctg ctgaccgcac ggcatagcta tattctgccg gttctggggc gtagcgagat
     1621 tgacatgcaa aaaagcggtg cgcaggcggt aaccgttgag gattcaatgt cgatgattca
     1681 tgcctcgcgt ggcgtgttaa aacccgccgg tgtaatgctg aaatcagagt gtgcagtggt
     1741 cgcgggaatc gcgcaggcag cactacccca cagcgtggta gcctgggagt atctggtgga
     1801 agattatgat cgcattcgca atgacattga agctgtgtta ccagagttcg ccgactataa
     1861 ccagcgcatc cgacatcctg gaggttttca cctgataaat gcagctgctg aaaggcgctg
     1921 gatgacgccg tcaggtaagg ctaatttcat taccagcaaa gggctgttag aagatccctc
     1981 ttcagcgttt aacagtaagc tggtcatggc gacagtacgt agccacgatc agtacaacac
     2041 gacgatttat ggtatggatg atcgctatcg aggggtattc ggtcaacgag atgtggtctt
     2101 tatgagtgct aaacaagcta aaatttgccg tgtaaaaaac ggcgaaagag ttaatcttat
     2161 tgcgcttacg ccagacggta agcgcagctc acgccgcatg gatagattaa aagtggtcat
     2221 ttaccctatg gctgaccgct cactggtgac ctattttcca gaatcgaatc acatgctaac
     2281 tcttgataac cacgatccat taagtggcat tcctggttat aaaagtattc cggttgaatt
     2341 agaaccatca aattaattcg cctcctcatt tcttctgctg tcatccgcac agcagaagaa
     2401 tttctcattg actattatcg cgcaatttgc tcacattgat taaattaaac cacacactat
     2461 aagatataaa cctctgccta cagctgtaag aaactccgct cagtactgaa gtgccagtcc
     2521 tatttcctct tttctccagc ctgttatatt aagcatactg attaacgatt tttaacgtta
     2581 tccgctaaat aaacatattt gaaatgtatg cgaccacagt gaaaaacaaa atcacgcaaa
     2641 gagacaacta taaagaaatc atgtctgtaa ttgtggttgt cttattactg acccttacgt
     2701 tgatagccat tttttcggca attgatcagc tgggtatttc agaaatgggt cgcattgcaa
     2761 gagatcttac acatttcatt atcaatagtt tgcaagactg aaaataaaca gcaaattata
     2821 aatatgaaat gttaaaaaag tatcgataaa aactttattg ttttaaggag ataaaatgtc
     2881 gctcgtttgt tctgttatat ttattcatca tgccttcaac gctaacattt tagataaaga
     2941 ttacgccttc tctgacggcg agatcctgat ggtagataac gctgttcgta cgcattttga
     3001 accttatgag cggcatttta aagagatcgg atttactgaa aataccatta aaaaatatct
     3061 acaatgcact aacatccaga cagtgacggt gcctgttcct gcgaagtttt tacgtgcttc
     3121 aaatgtaccg actggattgc ttaatgaaat gattgcttat cttaactcgg aagaacgcaa
     3181 tcatcataat ttttcagaac ttttgctttt ttcttgcctg tctatttttg ccgcatgcaa
     3241 aggtttcatt acactattaa ctaacggtgt gctatccgtt tccggaaaag tgagaaatat
     3301 tgtcaacatg aagctggcgc acccatggaa gctgaaagat atttgtgact gcctgtacat
     3361 cagtgaaagc ctgttgaaga aaaaactaaa gcaagagcaa acgacattct cacagattct
     3421 attagatgcc agaatgcagc acgcaaaaaa tttgatacgc gtagaaggtt cagtcaataa
     3481 aattgccgaa caatgtggtt atgccagtac atcttatttt atttatgcgt tccgcaaaca
     3541 tttcggcaac agtccgaaga gagtttctaa ggagtaccgt tgtcaaagtc acatgggtat
     3601 gaatacgggc aacacgatga gtgctttagc tatttgatta tttgctaacg agtagtcaac
     3661 cacgcacgct gcgtaagaat taaatggggc agccattccc tgccccgcgt tgtttttagg
     3721 cgatatattt attgaaataa ataagtgaca tccatcacat atttatgcac ttgcataacc
     3781 tgttgcagat tatttatgat ctcaattcgg cattttgtca gtaaaatgca ataatttatt
     3841 aaatatcaat aaattagt
//
LOCUS       DAZPAC010000316         3858 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZPAC010000316 DAZPAC010000000
VERSION     DAZPAC010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.318"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            115..510
                     /gene="caiF"
                     /locus_tag="ACHUPM_004208"
     CDS             115..510
                     /gene="caiF"
                     /locus_tag="ACHUPM_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR0871823.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(719..1309)
                     /gene="caiE"
                     /locus_tag="ACHUPM_004209"
     CDS             complement(719..1309)
                     /gene="caiE"
                     /locus_tag="ACHUPM_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR0871824.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="ACHUPM_004210"
     CDS             complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="ACHUPM_004210"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR0871825.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="ACHUPM_004211"
     CDS             complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="ACHUPM_004211"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR0871826.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          911 a         1049 c          942 g          956 t
ORIGIN      
        1 ttatcgacgg tgaagttgca tactatcgat atatccacaa ttttaatatg gccttgttta
       61 attgcttcaa aacgagtcat agccagactt ttaatttgtg aaactggagt tcgtatgtgt
      121 gaaggatatg ttgaaaaacc actctacttg ttaatcgccg aatggatgat ggctgaaaat
      181 cggtgggtga tagcaagaga gatctctatt catttcgata ttgaacacag caaggcggtt
      241 aataccctga cttatattct gtcggaagtc gcagaaataa gctgcgcagt taagatgatc
      301 cctaataagc tggaaggacg gggatgccag tgccagcgac tggtaaaagt ggtcgatatc
      361 gatgagcaaa tttacgcgcg cctgcgcaat aacagccggg ataaattagt cggcgtaaga
      421 aagacgccgc gtattcctgc cgttccgctc acggaactta accgcgagca gaagtggcag
      481 atgatgttgt caaagagtat gcgtcgttaa tgtcatttcg ttgataccac gcagcctggt
      541 tgctggatgc aatgctggcg catcttatcc agcctacggt tgctctccag ccgggttgga
      601 taaggcgttt atgccgcatc cgacagctat cgccggatgc gatgctggcg catcttatcc
      661 ggcctacggt tgctctccag cagagttgga taaggcgttt acgccgcatc cggcaagatc
      721 accgtttagg cgtcacatcc gtcgtcccct gcaaacgggg gcgattttcc tccatttgcc
      781 tcagtggctg tgtttcatgt aacgatgcat gacagcgccc gacaagatcc tgatactctt
      841 tggtattcaa ccgtttccag tgtaactcgt cgtcactaac actgcgtaca gcgcgggctg
      901 gcgtacccat caacaactgg cgtttctcgc cgtgaaagcc tgctttgaca aagctcatgg
      961 cggcaacaat gctctcttcg ccaatgaccg cgccatccat aatcacgctg ttcatcccga
     1021 ccaatgcatc gcgaccaatc acacaaccat gcaggatcgc tccgtgcccg atatggccgt
     1081 tttccccaac gatggtgtca gtgtcgcagt agccatgcat aatgcagcca tcctgaatat
     1141 tggctcccgc ttgcacgatc aaccgcccgt agtcaccacg cagtgaggcg agtgggccga
     1201 tgtagacacc ggctcccaca atcacatcgc caatcaagac ggcactggga tggacaaacg
     1261 ccgtcgggtg aaccaccgga attaacccct caaaggcgta atagctcacg gttgttaacg
     1321 tcctttccac accggatcgc gcttctcggc aaacgccagc ggcccttcaa tggcatcctc
     1381 cgaatgcaga accgacggat agtgtttcaa cacgccgctg cgaatatagc gatacgcttc
     1441 ttctaccggc atttcgctgg tggtgcggaa gatctctttc agcgccgcaa tcgccagcgg
     1501 ggcgctgtta accagctgct gcgccagttc gcgggcgtta tccatcagtt ccgcctggct
     1561 aaccacgcgg ttgactatcc cccaacgcag cgcctcttct gcgcccattc gtctgccggt
     1621 catcaccatt tcattgacga tggcaggcgg caggatcttc ggtagacgca gcacgccgcc
     1681 gctgtcagga acgatgccca gcttggcttc cggcagggcg aagctggcgt tatcggcaca
     1741 aacaataaaa tctgccgcca gcgccagttc gaaaccgccg tcaaaggcat agccgttcac
     1801 agctgcgata accggtttgt cgagattgaa aatttcggtt aatcccgcaa atccacccgg
     1861 gccaaagtca gcatccggtg cttcgccttc tgctgccgct tttaaatccc agcccgcgga
     1921 aaagaacttc tctccggcac cggtaataat ggcgacacgt aattgcggat cgtcacggaa
     1981 atttagaaat acttcgccca tttcaaagct ggtttttgca tcaatagcat tcgcttttgg
     2041 acgatcaagg gtaatttcca gaattgatcc attgcgggtc agatgtaatg attcactcat
     2101 tccttttctc catttttgct ttttcaggga cgacaacatc cctgcaaaaa atgcatattg
     2161 ttttatagcg tgattattag ttggcagggt agttccctgc tgtttcattt atttcagatt
     2221 ctttctaatt attttccccg agcaattacg tggcagatct tttctgatct ccagataaga
     2281 gggcacttta aatttcgcca tattttgttc gcagaagcgg aaaaattcct cttcgctcaa
     2341 tgtttcacct tcattcagca ccacaaatgc tttgatggct tcatcgcgaa tcgaatcttt
     2401 aatacccaca accacgatat cctgaatttt tggatgggtg gcgatgatat tttccagctc
     2461 cacgcaggag acattctcgc caccgcgttt gatcatattg cagcggcgat cgacgaaata
     2521 aaaaaagccc tcttcgtcgc agtatccggt atcgccggta tgtagccagc cgtcggcttc
     2581 cagcactttc gcagtggctt tcgggttgag aaaatactct ttgaagatgg ttttccctgg
     2641 tacgccttta atacagattt caccgatctc acctgccggg agcgggcgat tgtgatcgtc
     2701 gcggatctcc gcttcgtagc aaaaccccgc ccgaccaatc gacggccagc gtcgtttatc
     2761 gccagggcga tcgccgataa tgcccacaat ggtttccgtc atcccataag acgtcagcaa
     2821 gcgaacgccg aagcgttcac aaaacgcatc tttttcctgc tccgacaagt tgagataaaa
     2881 catcacttcc cgcaggcggt gttgccgatc gttcgctgaa ggcggctgcg ccatcaaggt
     2941 acggatcatc atcggaatac attcggtaac ggtggcgcgg tacttctgta cctgtcccca
     3001 gaaggcgcgg gcgctgtatt tctcgaccag cacaaaggtg gccccggcag aaaacgccgc
     3061 catcgccgca gtacactggc aatcgatatg aaacgcaggc attaccgtca ggtagacgtc
     3121 atcgtcacgc agtgcacact gccaggcgga gtaatatcca gcgaagcgca ggttgtaatg
     3181 ggtaatcacc acacctttcg gtcgggaggt ggtgccggag gtgaagagaa tttccgccgt
     3241 atcgtcagtc gatagcggcg gtgcatagca caaggtggca ggttgttgat ttttcagttg
     3301 agtaaacgaa ctcacgccat catcagcggg aagtgccaca tctgtcaggc aaatgtgccg
     3361 caattgagtg gcatcttcct gctgaatctg ttgatacata ggatagaatt gcgcactggt
     3421 caccagcagg cacgcctggc tattttgcag gatccacgcg ctttcctcgc gcaacaggcg
     3481 ggcgttaatc ggcaccataa tcgcgccaat ttttgccagc ccgaaccagc aaaagataaa
     3541 ttccgggcag ttgtcgagat gtagagcaac cttgtcgcct ttgcgaatcc ccagcgtata
     3601 aaacaggttt gtcgtgcggt taatctcctg atttaactca agataactat accggttaac
     3661 gactccgccg ctggattcac aaatcagcgc cgttttatga ccgtaaacgt ccgccagatc
     3721 gtcccacatt tgacgtagat gttgtccgcc aatgatatcc attgcacctc tatccatttt
     3781 tgttcgtttg ttattgggcg ggcgctagtc aggcaagccg actgacgcca cgcgtttagt
     3841 cctcaacttt ggccagac
//
LOCUS       DAZPAC010000317         3854 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZPAC010000317 DAZPAC010000000
VERSION     DAZPAC010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.319"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /gene="yneK"
                     /locus_tag="ACHUPM_004212"
     CDS             complement(<1..215)
                     /gene="yneK"
                     /locus_tag="ACHUPM_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR0871827.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRT"
     gene            complement(292..1173)
                     /locus_tag="ACHUPM_004213"
     CDS             complement(292..1173)
                     /locus_tag="ACHUPM_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0871828.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            1274..2662
                     /gene="sad"
                     /locus_tag="ACHUPM_004214"
     CDS             1274..2662
                     /gene="sad"
                     /locus_tag="ACHUPM_004214"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFR0871829.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2726..3652
                     /gene="glsB"
                     /locus_tag="ACHUPM_004215"
     CDS             2726..3652
                     /gene="glsB"
                     /locus_tag="ACHUPM_004215"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR0871830.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            3652..>3854
                     /locus_tag="ACHUPM_004216"
     CDS             3652..>3854
                     /locus_tag="ACHUPM_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HFR0871831.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPAGNDSNLLIVFYVQI"
BASE COUNT          943 a          896 c         1048 g          967 t
ORIGIN      
        1 gtcctctgaa tatatgataa gttatcaagg ttattaaata tcttttttgt ggctgagttg
       61 atgtttaaag acgtgcgcca ctcttcatca catattattg tacttagcgc atcaataaag
      121 tcctttaaaa cttcttttac ctgtggtgca atattacgaa cgggaaaatc atcaggaaga
      181 gatatgtaat tgctgatgct tactggcgta accatatttg ccccatatag tcataagtca
      241 tcggaacatc actatataga gcatattagc cgcaagaatt atcaaaatag ttcattggta
      301 actctgtttt gccgagtcag acacatccag tagttgaata aatgcctcaa gcggacgcgt
      361 tttcgcaccg cgacgccaga ccagccaggt tgttaaccaa cgccattgct cagctaacgg
      421 ccacgcttca acctggtgat gccccggcat actttccagc atagagcgtg gaataagcgc
      481 aatgcctgct cctgcgacca cacaggccaa catcccgtga tacgactcca tctcgtgaat
      541 agttcccgga gcggcaccgt cagcatgaaa ccagctctcg aagtggcgac gatacgaaca
      601 attggtgcgg aaggcataaa tgttactgcc gttaacctga ctggcgcgga ttactggcgc
      661 atgaccttgt ggcgtgacga tcatcagctc ctcgcggtat accggtatcc cgtcaatggc
      721 agtatggtta atgggcccat caataaatgc cgcattcagt tttccctcca gcactccttc
      781 cagcatagtg ccggaagggc cggtggaaag tgaaaactga attttgggat aacgatggtt
      841 gaattccgcc agcgtggccg gaatgcgcac tgctgcggtg ctttccagcg aaccaagaga
      901 aaataaacct tgcggctcat cgccagcgac aacgctccgc gcttcatcca ctaacgcgag
      961 aatttgttgg ctatagcgta aaaagttatg cccggcagga gagagacgta aacgctgatt
     1021 ctcgcgaata aacagatcta cccccagttc tgtttccagc tggcgtagac gggtagtgag
     1081 attcgacggt acgcgatgca cttttgctgc agcctgggta atgctgccag cttcggcaac
     1141 cgcgttgaac atctccagtt gggtcagatc cataacattc tcgattcgtg aataagtggc
     1201 ttaatattat tcattttaaa gcaagagtaa atctgcgtat cttcatacca tgactcataa
     1261 aggagatacc ccgatgacca ttactccggc aactcatgca atttcaataa atcctgccac
     1321 gggtgaacaa ctttctgtgc tgccgtgggc tggcgctaac gatatcgaaa acgcacttga
     1381 gctggcggca gcaggctttc gcgactggcg cgagacaaat atagattatc gtgctgaaaa
     1441 aatgcgtgat atcggtaagg ctctgcgcgc ccgtagcgaa gaaatggcgc aaatgatcac
     1501 ccgcgaaatg ggcaaaccaa tcaaccaggc gcgcgctgaa gtggcgaaat cggcgaattt
     1561 gtgtgactgg tatgcagaac atggtccggc aatgctgaag gcggaaccta cactggtgga
     1621 aaatcagcag gcggttattg agtatcgacc gttggggacg attctggcga ttatgccgtg
     1681 gaattttccg ttatggcagg tgatgcgtgg cgctgttccc atcattcttg caggtaacgg
     1741 ctacttactt aaacatgcgc cgaatgtgat gggatgtgct cagctcattg cccaggtgtt
     1801 taaagatgcg ggaatcccgc aaggcgtata tggctggctg aatgccgaca acgacggtgt
     1861 cagtcagatg attaaagact cgcgcattgc tgctgtcacg gtgaccggaa gtgttcgtgc
     1921 tggagcggct attggcgcac aggctggagc ggcactgaaa aaatgcgtac tggaactggg
     1981 cggttcggat ccgtttattg tgcttaacga tgccgatctg gaactggcgg ttaaagcggc
     2041 ggtagccgga cgttatcaga ataccggaca ggtatgtgca gcggcaaaac gctttattat
     2101 cgaagaggga attgcttctg catttaccga acgttttgtg gcagctgcgg cagccttgaa
     2161 aatgggcgat ccccgtgacg aagagaacac tctcggacca atggctcgtt ttgatttacg
     2221 tgatgagctg catcatcagg tagagaaaac cctggcgcag ggtgcgcgtt tgttactggg
     2281 cggggaaaag atggctgggg caggtaacta ctatccgcca acggttctgg cgaatgttac
     2341 cccagaaatg accgcgtttc gggaagaaat gtttggcccc gttgcggcaa tcaccgttgc
     2401 gaaagatgca gaacatgcgc tggaactggc taatgatagt gagttcggcc tttcagcgac
     2461 catttttacc accgacgaaa cacaggccag acagatggcg gcacgtctgg aatgcggtgg
     2521 ggtgtttatc aatggttatt gtgccagcga cgcgcgagtg gcctttggtg gcgtgaaaaa
     2581 gagtggcttt ggtcgtgagc tttcccattt cggcttacac gaattctgta atatccagac
     2641 ggtgtggaaa gaccggatct gacgcgacat attcagctct gatatactcg caggtctttt
     2701 cagacctgcg gtccaggagt agaaagtggc agtcgccatg gataatgcaa ttttagaaaa
     2761 catcttgcgg caagtgcggc cgctcattgg tcagggtaaa gtcgcggatt atattccggc
     2821 gctggctaca gtagacggtt cccgattggg cattgctatc tgtaccgttg acggacagct
     2881 ttttcaggcc ggagacgcgc aagaacgttt ttccattcag tctatttcca aagtgctgag
     2941 tctcgttgtc gccatgcgtc attactccga agaggaaatc tggcaacgcg tcggcaaaga
     3001 tccgtctgga tcaccgttca attctttagt gcaactggaa atggaacagg gtattccgcg
     3061 taatccgttt attaatgctg gtgcgctggt ggtctgcgat atgttgcaag ggcgattaag
     3121 cgcaccacgg caacgtatgc tggaagtcgt gcgcggctta agcggtgtgt ctgatatttc
     3181 atacgatacg gtggtagcgc gttccgaatt tgaacattcc gcgcgaaatg cggctatcgc
     3241 ctggctgatg aagtcgtttg gcaatttcca tcatgacgtg acaaccgttc tgcaaaacta
     3301 ctttcattac tgcgctctga aaatgagctg tgtagagctg gctcggacgt ttgtctttct
     3361 ggctaatcag gggaaagcta ttcatattga tgaacctgta gtgacgccaa tgcaggcgcg
     3421 gcaaattaac gcgctgatgg cgaccagtgg tatgtaccag aacgcggggg agtttgcctg
     3481 gcgggtggga ctaccggcga aatctggcgt tggtggcggt attgtggcga ttgttccgca
     3541 tgaaatggcc atcgcagtct ggagtccgga actggatgat gcaggtaact cgcttgcggg
     3601 tatcgccgtc cttgaacaat tgacgaaaca gttaggacgt tcggtttatt aatgcagtct
     3661 ctcgatccac tcttcgcgcg tttatcccgt tcaaaatttc gctctcgctt tcgtctgggc
     3721 atgaaagagc gtcagtattg cctggagaaa ggcgcgccag tcatcgaaca acatgcggcg
     3781 gattttgtcg ctaaacgtct tgctccggca ggtaatgact ccaacttatt gatagtgttt
     3841 tatgttcaga taat
//
LOCUS       DAZPAC010000318         3838 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZPAC010000318 DAZPAC010000000
VERSION     DAZPAC010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.321"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1358
                     /gene="dcuC"
                     /locus_tag="ACHUPM_004217"
     CDS             <1..1358
                     /gene="dcuC"
                     /locus_tag="ACHUPM_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0871832.1"
                     /translation="VVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSSQ
                     ASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQY
                     INSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPA
                     AIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHI
                     SHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILEF
                     LRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFG
                     SASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASN
                     LGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTG
                     K"
     gene            complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="ACHUPM_004218"
                     /gene_synonym="citB"
     CDS             complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="ACHUPM_004218"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFR0871833.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="ACHUPM_004219"
     CDS             complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="ACHUPM_004219"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFR0871834.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          892 a          996 c          919 g         1031 t
ORIGIN      
        1 gggttgtggt tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg
       61 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      121 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      181 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg
      241 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      301 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga
      361 tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc
      421 cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg
      481 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaag
      541 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
      601 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
      661 atgaaatgtt agatgtcagt gaaatcacca ccactgctcc tgcgttttat gccattttgc
      721 cgttcacgcc gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca
      781 tcatcactat tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct
      841 ttaataccca gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt
      901 ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca
      961 tcggctttat tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc
     1021 tgatgctggt attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg
     1081 cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc
     1141 cggcgtattt gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg
     1201 tttctggcgt agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa
     1261 aacgcacctc ggtgccggtg cttgttggtt tggtgattgt tatcgttgct acagagctga
     1321 tggtgccagg aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt
     1381 tcctccggct ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc
     1441 gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc
     1501 agtggtgcag ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg
     1561 ctctttaaac agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggtagctc
     1621 gtccttaggt tcaccgcgag cataagcatt aaacatctca tcaatttgct tctggctggc
     1681 gctatcaata ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag
     1741 ccgttcatag gcaatgggct taatgagata atcaaataca ccacaacgta cggcttcaga
     1801 caccgtttcc atatcgctgg ctgcagtggt aaacaccacg tcgccgggat aatgcgcctg
     1861 caccagttca tgcagtaaat taatccctct accgtcagga agatagttat cgagcaagat
     1921 tagccccggc ttaaaacgct cgatcatcat tcgggcctgc gccagatttc ccgccagtaa
     1981 tatctgactg aatccgggaa tgtgacgaat atattccgca tgcatctctg ccagcggcgt
     2041 ttcgtcctca acgatcaata gggttaatgg agctgtcatt aggtttcact ttcggaatat
     2101 agattgaaaa taaggtaccg cagggatcat tatcttcgag agtgataaca ccaccgcagc
     2161 gcgttacgta gctggcaatc aagtacaacc caatgccatg ttcaccgggc tcgtcagcac
     2221 gcgtactgac cccctgctca aatattttgt ctcttagaga ctctggaacg ccgcagccct
     2281 gatcggcgac ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgatct
     2341 tgtttccttc atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgccaacaa
     2401 tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga
     2461 cgatgatcat ttttagcccc agttcgcggg cgcgctgcac tttaccaaaa agcagccccg
     2521 ccacctggcg atcggcaaac gcctcacgca ggctgtcaat aagctgttgc tgggcctgag
     2581 actccccctg caccatcgcc agcacgcgat catactcttt catctgcaac agaccattga
     2641 gcgtcgacat ccaattgtga tgctcgtgtc gcaatgtacg aaggctctcg acgtattgtt
     2701 ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc tttactacga aagctgataa
     2761 tggcccccag caaatcatca cctgaacgaa tagcttcccg gttggcaata acgctcagac
     2821 cattaaagtt cgccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg
     2881 cgggcctgac cacttcagca atgggtttac ccaaccattg ccgtccggga gagctcagcc
     2941 ccagcatctt tcttgcgtta cgattgatgg cggtaatgta accatgcgga tccaccgcaa
     3001 tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg acggaccacg cgtgcgattt
     3061 gctttggctc catgcccatc atttgtcgac ggatatgcgc ggccaggaac cacgacagca
     3121 acatcagaat ccctaacagc acgacaaaca cgcctgccat cggtaataaa aactcagccc
     3181 gccagctatc gattttactc accaggtagc caatcgacac cacgccgatg acttttccat
     3241 cgtcatcaaa gattggcgtt ttggcgcgca tcgccatccc cattgcccct ttcccggtaa
     3301 tgaagtagct ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa
     3361 ttttctccgg attaagatgg taaaggcgga tcgagtgccg gtcgccaatc accacataat
     3421 caaaatcggt atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg
     3481 ttttcaccgc agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca
     3541 tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg
     3601 ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgcca
     3661 gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt ttccttgcgg
     3721 gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata catagatatt
     3781 aattatgatc gtggcaaatt ttattcatta aatcaattaa atcacttaaa cctgcagg
//
LOCUS       DAZPAC010000319         3833 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZPAC010000319 DAZPAC010000000
VERSION     DAZPAC010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.322"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            89..343
                     /gene="mobC"
                     /locus_tag="ACHUPM_004220"
     CDS             89..343
                     /gene="mobC"
                     /locus_tag="ACHUPM_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="HFR0871835.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
     gene            877..1578
                     /locus_tag="ACHUPM_004221"
     CDS             877..1578
                     /locus_tag="ACHUPM_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0871836.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPD
                     EQKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRA
                     QYFKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTG
                     DRTIAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
     gene            complement(1836..>3833)
                     /locus_tag="ACHUPM_004222"
     CDS             complement(1836..>3833)
                     /locus_tag="ACHUPM_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR0871837.1"
                     /translation="AVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPH
                     ELPQDAARETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKV
                     RDWNDRTHAETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAM
                     ETRGIETERGEQNRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYT
                     LRDIIEALPKDSNSAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSP
                     FSSGFSRIPLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQW
                     QQWGESGLAALRAQLLKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGY
                     GEAPMPGNGEMTCYLLLHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEI
                     GQRPPLQRVYSSATFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAA
                     RKVDFMQGRLPPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPS
                     ADNRHLVISALTDKGLRYAGYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTP
                     EKQPDHLFRSLGFNRTWEEWQREEDARTHTTERRHDRGWSQ"
BASE COUNT          909 a          970 c         1002 g          952 t
ORIGIN      
        1 gtgatttgcc gtgcattatg ccagctcagt ttttaaacta ccagtgacgc gctaaaatgc
       61 gatttttcat cgcgcacgga gttacatcat gagcagaacg ctcgaacaga agattgctga
      121 tgctgaggcg agattacagc gcctgaaggc gaagagccgg agcctggaca cagcgcagaa
      181 ggttgttgtg ggtgctgcat tgctggcaaa ggtcagaaag ccggaggagg tgcagttgcg
      241 tgcctggtta ctccagttcc tgaaggcaga ggtgacacga caggctgatg tgacgcgcat
      301 actgccgctg attaacgagc tggaggcgct gccagaacag tgattgtatc gtccgctcgc
      361 cgcagcccca gcgaccgagc gttagcgagc gagtgggcga ggaagcggaa gtgaatcagt
      421 cgccaggttg agcacatacg cacggttttt cggcaggtga tatgcgctga acccgcatga
      481 atactgggtt tccacagggt tggagggggt gtattcgaaa cttgcaattc tgctttattg
      541 caacgcattg tttttattgg ttttttgttt tcagcacccc aagttatcca caggctcgcc
      601 gttaagtttt taagatctta tttttagatc ttttttttta aaaccttttt aggtcttgta
      661 acaccatgat tttaaagatg ttctggcaag ataagtggag cttttggggg tgaaaggtgg
      721 agcttttggg ggtgaaaggt ggagcttttg ggggtgaaag gtggagcttt tgggggtgta
      781 atccacagaa aaagggggtt gcaaaaaaaa aacttccacc catatgataa gtggcgtaca
      841 gtaaaaaaac gccaccaata gcaacgagca agcgaaatgt cagatctgat agcttacaaa
      901 tcaaacgccc ttatcgaggc aagctacaag ctcactttgc aggaacagcg cttcctgctg
      961 gtctgtatca gccgcctaaa atccggagaa gattcccctt cgccggatga acaaaaaacc
     1021 atgaccatca cggctgcaga gtttcatgat gcctttcctg acatgggacg gcagcacgct
     1081 gaagctaggc tcaaggaagc gatagatcgc ctctgggaca ggtcagtaat catcaagaac
     1141 gaggaaaaac gagaagagtt ccgctgggtg cagtaccggg cgcagtattt caaaggtgag
     1201 gcgaaagtag atatcacctt ctccgatgcc atcatgccgt atctgacaca gctcaagggg
     1261 cagtttacgc gggtagtcgt gaagaacgtc tccagccttt ccagcaccta cagcatccgc
     1321 atttacgagc tgctccagca gttccgcagc accggtgaca gaacgatagc tatcgatgat
     1381 ttccgaacca tgcttgccgt tgaagataaa tatcaacaat tcaaagactt gaataagatg
     1441 ataatcaagc cagccatcgc cgaactgaac gaaaaaagcg atctggttgt aactgttgag
     1501 acgatcaaaa aggggcgcac ggttgtagcc ctgcattttc gcttcaagga agacaaacag
     1561 atcaagatga cgatctaaaa tcgattttaa ggcacctaaa acgctccctg ctgcgttttt
     1621 tttgtgttag tagtgcgaag gtactattga agtgaaaaat tgcgtcaaaa acgctctgat
     1681 gcagcgaaaa aagacccccc aatttttggg ggcggaaaaa caaaactatg cttgcgttat
     1741 gtgagtgtgc ttcttgcatt catccctgtc gatgaacatc gaagaacatc gacgaacata
     1801 acacgatgtt aaacaaaaca aaatattgtt cacattcact gtgaccaacc gcgatcatgt
     1861 ctcctctcgg tggtgtgtgt tcgtgcatct tcctcgcgct gccattcctc ccaggtacga
     1921 ttaaaaccca gcgagcggaa cagatgatcc ggctgttttt ctggtgtgtt gacggcagca
     1981 aaaagcggtg cctcacgggt tttcagatga aactgcatca gttctggtgc aggtgatatg
     2041 tggctgtctg catcgtgatt tacggcaaca ccataccctg cataccgtaa ccccttatcg
     2101 gtcagcgcac tgatgaccag atgcctgtta tcggctgagg gaacggcaaa cgcgggatac
     2161 agcgtaccat taagccggat aacaccgccc agagagtcat ccacgcccat gaccagcaaa
     2221 agctgaagct ggcgggtttc ccggtcgaca gaactcatga ccggcatcag cgtcggtggc
     2281 agacgtccct gcataaaatc caccttcctt gccgctggtg atggcatggc tggctggcgg
     2341 tcttctccaa gacagaaaga ctccggccat gtcttcggct ggcggagatt gcgggtatgt
     2401 tgcggccatg tctggcaaac ctgcgtcagc agttgctcaa aggttgcaga ggaataaaca
     2461 cgttgcagtg gcggacgttg gccgatttcg cttcggtcac agatataacc actcctgtca
     2521 cgcattaaat caacactctc aaagattctg gtgcggtatt tctccagctc gttcccccag
     2581 agcgtatatt ctccgttccg gttgtgaagc agaaggtaac aggtcatttc cccgttacca
     2641 ggcatgggag cctcgccata ccccgtaacg actgctctca gcacccctga atccataacg
     2701 gcattgtcat ttttcctgag ctgctccgcg cgactacgtt cagtatcttc ctgttccttc
     2761 cggcgcaatt cctcttcacg tttaagcaac tgggctctga gagctgcaag gccggattcc
     2821 ccccactgct gccactgttt ttcaaatgcc atctgactcg cgggaatgtc ctgtttctcc
     2881 accttccggt aatgctgtcg taatttttcc atttgctggc tcagagactg gatttttccc
     2941 tgctcactac ggtattccgg tgccgcccat tgcatcaggg gaatacggga aaaaccggac
     3001 gaaaacggcg atgatttttt caggctggca gcatggctca tcgcttcctt tcgttcggtg
     3061 ttcagctctt cccagcgtct gcgcgtcgcc tgcatatctg ccatcatcga catggcatta
     3121 ctggtgagtt tccatgccga gttactgtcc ttcggcaacg cttcgataat gtcacgtagt
     3181 gtgtacgaag ttgcgttgtc aggaagcccg atattcagaa ggtcagccac acggcgggca
     3241 ggctctgtca gccctccgtt cctgagttcg gtgcgggaaa tctgtatttc caggttaagc
     3301 ctgttaatca gacgattctg ttcgccgcgt tctgtttcga taccgcgtgt ttccatcgca
     3361 caggcagact ttccgaggtg aataccaggc gttttctcaa gcccctgtcg ctcgtatgaa
     3421 cgatggtcta tctcttcctg ataaccggcg ttcgccagtg ccctgtttgc atggtcagcc
     3481 catgatgccc gccacgtctc ggcatgtgtc cggtcgttcc agtccctgac ctttccgctg
     3541 aacccagcag gtccgatagc cctcgtggtc agcatgatgt gcgcatgggg atttgtttta
     3601 tccatgtgat ggaacgccac atcagcaatc atgccctgac tgacaaagtt ttctctgaca
     3661 aaggcgagaa cggtttcccg tgcggcatcc tgcggcagtt cgtgggggat ggcgagttca
     3721 atttcccgcg caagttgtga gttcttccgg cgttcggcct tctcaacggc attccagagt
     3781 tcggcacggt tcgtacacca ggcaggggcg gtggcggggg tcagaatcac agc
//
LOCUS       DAZPAC010000320         3832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZPAC010000320 DAZPAC010000000
VERSION     DAZPAC010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.323"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            89..343
                     /gene="mobC"
                     /locus_tag="ACHUPM_004223"
     CDS             89..343
                     /gene="mobC"
                     /locus_tag="ACHUPM_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="HFR0871838.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
     gene            875..1567
                     /locus_tag="ACHUPM_004224"
     CDS             875..1567
                     /locus_tag="ACHUPM_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0871839.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQK
                     TMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYA
                     KGEAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERI
                     IALNDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHF
                     RFKEDKQIKMTI"
     gene            complement(1823..>3832)
                     /locus_tag="ACHUPM_004225"
     CDS             complement(1823..>3832)
                     /locus_tag="ACHUPM_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR0871840.1"
                     /translation="AVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPR
                     ELPQDAARETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKV
                     RDWNDRTHAETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAM
                     ETRGIETERGEQNRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYT
                     LRDIIEALPKDSNAAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSP
                     FSSGFSRIPLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQW
                     QQWGASGLAALRVQLLKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAV
                     VTGYGEAPMPGNGEMTCYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICD
                     RSEIGQHPPLQRVYSSATFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMP
                     SLAARKVDFTQGRLLPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAF
                     AVPSADNSQLVISALTDKGLRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAA
                     VNTPEKQPDHLFRSLGFNRTWDEWRREEDARTHTTERRHDRGWSQ"
BASE COUNT          929 a          921 c          985 g          997 t
ORIGIN      
        1 gtgatttgtc gcgcattatg ccagctcagt ttttaaacta ccagtgacgc gctaaaatgc
       61 gatttttcat cgcgcacgga gttacatcat gagcagaacg ctcgaacaga agattgctga
      121 tgctgaggcg agattacagc gcctgaaggc gaagagccgg agcctggaca cagcgcagaa
      181 ggttgttgtg ggtgcagcat tgctggcaaa ggtcagaaag ccggaggagg tgcagttgcg
      241 tgcctggtta ctccagttcc tgaaggcaga ggtgacacga caggctgatg tgacgcgcat
      301 actgccgctg attaacgagc tggaggcgct gccaggacag tgattgtatc gtccgctcgc
      361 cgcagcccca gcgaccgagc gttagcgagc gagtgggcga ggaagcggaa gtgaatccgt
      421 cgccaggttg agcacatacg cacggttttt cggtaggtga tatgcgctga acccgcatga
      481 atactggatt tccacagggt tggaagggga gtattcggaa cttgcaattc tgctttcttg
      541 caacacattg tttttattgg ttttttgttt tcagcgcccc aagttatcca caggctcgcc
      601 gttaagtttt tagatcttgt ttttaagatc ttgtttttaa tacttgtttt agggtcaaaa
      661 ttcattaata aaatcaatat gttatagagg tatatgtgga ggaatttaag gtaaaggtgg
      721 aggaatttaa ggtgtttagg tggaggaatt taaggtaaag gtggaggaat ttaaggtgtt
      781 attcacagaa atgtgaggat ataaaaaaaa atattccaca tttcttattt gtggggcaca
      841 ttaaagcatc accacatttg aacgggatct ttgtatgtcg gacttgattg cctacaagtc
      901 gaatgctttg gttgaggcca gctacaagtt gaccctacag gaacaacgtt ttctgttgct
      961 gtgtatcagt cgattgaaat ctggtgctga tgctgaattg caaaagacaa tgaccattac
     1021 cgctgcggaa tattttgatt cattccctga catggggcgg aaaaatgccg aggttcagtt
     1081 acaggaagcg atcgatcgtt tatgggacag gtcgatcatt ctcaagaatg acgagaagcg
     1141 tgaggagttc cgctggatcc agtatcgggc acagtacgct aaaggtgagg ccaaggctcg
     1201 aataaccttt tctgacgcgg taatgcctta tctgacgcaa ctgaaggggc aattcacgcg
     1261 cgttgttata aaaaacatat caggtttatc aagtagttac agtataagaa tatacgagtt
     1321 cctccagcaa ttccgcagta cgggagagag aattattgct cttaatgact ttcgttcaat
     1381 gttagggatt gaaaataaat acaaacaatt cagagactta aataaaatac taataaaacc
     1441 ctgtgttgac gaactgaaca aaaaaagcga cctggctgta acggttgaga cgatcaaaaa
     1501 ggggcgcacg gttgtagccc tgcattttcg cttcaaggaa gacaaacaga tcaagatgac
     1561 gatctaaaat cgattttaag gcacctaaaa cgctccctgc tgcgtttttt ttgtgttagt
     1621 agtgcgaagg tactattgaa gtgaaaaatt gcgtcaaaag cgctctaatg cagcgaaaaa
     1681 agaaccccca atttttgggg gcggaaaaac aaaactatgc ttgcattatg tgtgtgcttc
     1741 ttgcattcat ccctgtcgat gaacatagaa gaacatcgac gaacataaca cgatgttaaa
     1801 caaaacaaaa tattgttcac attcactgtg accaaccgcg atcatgtctc ctctcggtgg
     1861 tgtgtgttct tgcatcttcc tcgcgccgcc attcatccca ggtacgattg aaacccagag
     1921 agcggaacag atgatccggc tgtttttctg gtgtgttgac ggcagcaaaa agcggtgcct
     1981 cacgggtttt cagatgaaac tccataagtt ccggcgcagg tgatatgtgg ctgtctgcat
     2041 cgtgatttac ggcaacacca taccctgcat accgtaaccc cttatcggtc agtgcactga
     2101 tgaccagctg gctgttatcg gctgagggaa cggcaaacgc gggatacagc gtaccattaa
     2161 gccggacaac accgcccaga gagtcatcca cgcccatgac cagcaaaagc tgaagctggc
     2221 gggtttcccg gtcgacagaa ctcattaccg gcatcagcgt cggtagcaga cgtccctgcg
     2281 taaaatccac cttccttgct gccagtgatg gcatggctgg ctggcggtct tctccaagac
     2341 agaaagactc cggccatgtc ttcggctggc ggagattgcg ggtatattgc ggccatgtct
     2401 ggcaaacctg cgtcagaagt tgctcaaagg tcgcagagga ataaacacgt tgcagtggtg
     2461 gatgttgacc gatttcgctt cggtcacaga tataaccact cctgtcacgc attaaatcaa
     2521 cacgctcaaa gattctggtg cggtatttct ccagctcgtt cccccagagc gtgtattctc
     2581 cgttccggtt gtgaagcaga aggtaacagg tcatttcccc gttaccaggc atgggggcct
     2641 cgccataccc cgtaacgact gcgctcagta cccctgaatc cataacggca ttgtcatttt
     2701 tcctgagctg ttccgctcgt cgctccgcgc gactacgttc tgtatcctcc tgctccttcc
     2761 ggcgcaattc ctcttcacgt ttaagcaact ggactctgag agctgcaagg ccggatgccc
     2821 cccactgctg ccactgtttt tcaaatgccg tctgactcgc gggaatgtcc tgtttctcca
     2881 ccttccggta atgctgtcgc aatttttcca tttgctggct cagcgactgg atttttccct
     2941 gttcgctacg gtattccggt gccgcccatt gcatcagggg aatacgggaa aaaccggacg
     3001 aaaacggcga tgattttttc aggctggcag catggctcat cgcttccttt cgttcggtgt
     3061 tcagctcttc ccagcgtctg cgcgtcgcct gcatatctgc catcatcgac atggcattac
     3121 tggtgagttt ccatgccgca ttactgtcct tcggcaacgc ttcgatgatg tcacgtagtg
     3181 tgtacgaagt tgcgttgtca ggaagcacga tattcagaag gtcagccaca cggcgggcag
     3241 gctctgtcag cccgctgttc ctgagttcag tgcgggaaat ctgtatttcc aggttaagcc
     3301 tgttaatcag acgattctgt tcgccgcgtt ctgtttcgat accgcgtgtt tccatcgcac
     3361 aggcagactt tccgaggtga ataccaggcg ttttctcaag cccctgtcgc tcgtatgaac
     3421 gatggtctat ctcttcctga taaccggcgt tcgccagtgc tctgtttgca tggtcagccc
     3481 atgatgcacg ccacgtctcg gcatgtgtcc ggtcgttcca gtccctgacc tttccgccga
     3541 acccagcagg tccgacagcc ctcgtggtca gcatgatgtg cgcatgggga tttgttttat
     3601 ccatgtgatg gaacgccaca tcagcaatca tgccctgact gacaaagttt tctctgacaa
     3661 aggcgagaac ggtttcccgt gcggcatcct gcggcagttc acgggggata gcgagttcaa
     3721 tttcccgcgc aagttgtgag ttcttccggc attcggcctt ctcaacggca ttccagagtt
     3781 cggcacggtt cgtacaccag gcaggggctg tcgcgggggt cagaatcaca gc
//
LOCUS       DAZPAC010000321         3832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZPAC010000321 DAZPAC010000000
VERSION     DAZPAC010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.324"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..266)
                     /locus_tag="ACHUPM_004226"
     CDS             complement(<1..266)
                     /locus_tag="ACHUPM_004226"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0871841.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            353..760
                     /gene="nudG"
                     /locus_tag="ACHUPM_004227"
     CDS             353..760
                     /gene="nudG"
                     /locus_tag="ACHUPM_004227"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR0871842.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(726..998)
                     /locus_tag="ACHUPM_004228"
     CDS             complement(726..998)
                     /locus_tag="ACHUPM_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR0871843.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            1234..2577
                     /gene="gdhA"
                     /locus_tag="ACHUPM_004229"
     CDS             1234..2577
                     /gene="gdhA"
                     /locus_tag="ACHUPM_004229"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR0871844.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2694..3733)
                     /locus_tag="ACHUPM_004230"
                     /pseudo
     CDS             complement(2694..3733)
                     /locus_tag="ACHUPM_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
BASE COUNT          997 a          932 c          917 g          986 t
ORIGIN      
        1 tcaagagaaa tatcaagaaa gccgcccgcg tcggtaagct ctctgcgccg cgccagcgca
       61 ccgtccagac catcaagcag cctgttcaac aaaatgacga ctagcgccgc cagataccag
      121 cccagcgcca gaaacggcag agccagcacg ccgatggcaa atcccactaa tgttaaacca
      181 tctggcgtaa tgcccggttt atcaagaacc cgcacgcact gatgcaacaa cggtttaatc
      241 cggggatgaa gatggcggtc tagcacaggc actccttaaa tataaagcct ttctgattga
      301 gcaacagtgc ggatattatg gcatttttcg cttatctgcc cgtgtgtaat ttatgaaaat
      361 gattgaagtt gttgccgcca tcattgaacg tgatggcaaa attttactcg cgcaacgccc
      421 cgcccagagc gatcaggcgg gattatggga gtttgccgat ggtaaagtcg agctggatga
      481 aagccagcag caggcactgg tgcgtgagtt aaacgaagaa ttgggcatcg aagcaactgt
      541 gggtgagtat gttgccagcc atcagcgaga agtttcgggc cggattatcc atcttcatgc
      601 ctggcacgta cccgacttcc acgggacgtt acaggcacat gaacatcagg cgctggtctg
      661 gtgctcacct gaagaggcgc tgcaatatcc gctggcccct gctgacattc cactattaga
      721 ggcgtttatg gctttacgcg ccgccagagc agcggattag tgctaagggt tttgtcatcc
      781 cgctggcatt gcagcagtat tccttcggct ttaattaccg ccccttcaga ataattttga
      841 tcctgataaa cgcagcactg agtacagggc tgcgctgact gcccgcctga actgaacact
      901 tctggcggta cgtttacctc cacgtccgga cgataatgcg ggttagccag tgcaattaat
      961 ggaaatgcta atactacggc gaacaatgct cgactcacag ggaactcctt aacgttattg
     1021 tctctgcaac tgataacggt agccgggtgg caaaacttta gcgtctgagt tatcgcattt
     1081 ggttatgaga ttactctcgt tattaatttg ctttcccggg tcattttttt cttgcttacc
     1141 gtcacattct tgatggtata gtcgaaaact gcaaaagcac atgacataaa caacataagc
     1201 acaatcgtat taatatataa gggttttata tctatggatc agacatattc tctggagtca
     1261 ttcctcaacc atgtccaaaa gcgcgacccg aatcaaaccg agttcgcgca agccgttcgt
     1321 gaagtaatga ccacactctg gccttttctt gaacaaaatc caaaatatcg ccagatgtca
     1381 ttactggagc gtctggttga accggagcgc gtgatccagt ttcgcgtggt atgggttgat
     1441 gatcgcaacc agatacaggt caaccgtgca tggcgtgtgc agttcagctc tgccatcggc
     1501 ccgtacaaag gcggtatgcg cttccatccg tcagttaacc tttccattct caaattcctc
     1561 ggctttgaac aaaccttcaa aaatgccctg actaccctgc cgatgggcgg tggtaaaggc
     1621 ggcagcgatt tcgatccgaa aggaaaaagc gaaggtgaag tgatgcgttt ttgccaggcg
     1681 ctgatgaccg agctttatcg tcatctgggc gcggataccg acgttccggc aggtgatatc
     1741 ggcgttggtg gtcgtgaagt cggctttatg gcggggatga tgaaaaagct ctccaacaat
     1801 accgcctgcg tcttcaccgg taagggcctt tcatttggcg gtagtcttat tcgcccggaa
     1861 gctaccggct acggtctggt ttatttcaca gaagcaatgt taaaacgcca cggtatgggt
     1921 tttgaaggga tgcgcgtttc cgtttctggc tccggcaacg tcgcccagta cgctatcgaa
     1981 aaagcgatgg aatttggtgc tcgtgtgatc actgcgtcag actccagcgg cactgtagtt
     2041 gatgaaagcg gattcacgaa agagaaactg gcacgtctta tcgaaatcaa agccagccgc
     2101 gatggtcgag tggcagatta cgccaaagaa tttggtctgg tctatctcga aggccaacag
     2161 ccgtggtctg taccggttga tatcgccctg ccttgcgcca cccagaacga actggatgtt
     2221 gacgccgcgc atcagcttat cgccaatggc gttaaagccg tcgctgaagg ggcaaatatg
     2281 ccgaccacca tcgaagcgac tgaactgttc cagcaggcag gcgtactgtt tgcaccgggt
     2341 aaagcggcta atgctggcgg tgtagcaacg tcgggcctgg aaatggcaca aaacgctgcg
     2401 cgcctgggct ggaaagccga gaaagttgac gcacgtttgc atcacatcat gctggatatc
     2461 caccatgcct gtgttgagca tggtggtgaa ggtgagcaaa ccaactacgt gcagggcgcg
     2521 aacattgccg gttttgtgaa ggttgccgat gcgatgctgg cgcagggtgt gatttaagtt
     2581 gtaaatgcct gatggcgcta cgcttatcag gcctacaaat gggcacaatt cattgcagtt
     2641 acgctctaat gtaggccggg caagcgcagc gcccccggca aaatttcagg cgtttatgag
     2701 tatttaacgg atgatgctcc ccagggaaca tttcttatgg gccaacggca tttcttactg
     2761 tagtgctccc aaaattgctt gtcgtaacga taacacgctt caagttcagc atccgttaac
     2821 tttctgcgat agcagcagat atgccagtaa agaaacccca ttagactatt tttttgataa
     2881 tcttcttcgc tttcgaacaa ctcgtgcgcc tttcgagaag caagcattat ataatgccag
     2941 gccagttctt cttcaattgt cccgttttga aaagctgtgc ttgatatcga ggtcatccat
     3001 gataattccg ccgcccatat tagcttcgcc gaggatttac cggagcgatt attagcgcaa
     3061 tcagagatat agtctgaggg aaaaacagca aatttattca acaaggcgat aacctgctct
     3121 ggggcttcct ccatgtttgc tttaaaggta ttggctccat ggtcgccaga aagaaaatgc
     3181 tccattaggc acaataactt tcgctatctt cgatacccca ttgattctct aaagactcgc
     3241 gtcttttact gatgatatcg atcgagtcaa aaggaagcac atgatattgg aaggtatctt
     3301 taccaggttc aggctttcgc ggccagaact ctagcgtttc agaccattgc ttatgataga
     3361 atcgataagg tgcgatcaat tgtagcgcct gtaacttctc gatactgagc ggctcaatac
     3421 ctttagcctg ataataatgc agttgttctt tttttgcttt aaaaccggcc cgaacaataa
     3481 gccccatcat aattaataga taaagaaaag agcatcccgc ggtaatcagg cctctttcat
     3541 tcaaaccgtt ggatgttatc gctgcgaaca caaacattac agcgacaaca catgttaaat
     3601 aaaaccccca cttacaaagc agcatggcct tattttcttt aatcatccgt tcaaaattac
     3661 tattaaatat ttcccagcca ttaaaagaat acttctcgct cccaggatgg ttttgtaata
     3721 aaactttttt catcacattc cctgttatta catactttag aggcgataat tttatccatg
     3781 caaaaaaaat atccaacgaa ttttcttgat ccgctggata tttcagaata tg
//
LOCUS       DAZPAC010000322         3798 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZPAC010000322 DAZPAC010000000
VERSION     DAZPAC010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.325"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..82
                     /locus_tag="ACHUPM_004231"
     CDS             <1..82
                     /locus_tag="ACHUPM_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871845.1"
                     /translation="REWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(99..908)
                     /gene="proC"
                     /locus_tag="ACHUPM_004232"
     CDS             complement(99..908)
                     /gene="proC"
                     /locus_tag="ACHUPM_004232"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR0871846.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1028..1486
                     /locus_tag="ACHUPM_004233"
     CDS             1028..1486
                     /locus_tag="ACHUPM_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR0871847.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1669..2193
                     /gene="aroL"
                     /locus_tag="ACHUPM_004234"
     CDS             1669..2193
                     /gene="aroL"
                     /locus_tag="ACHUPM_004234"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR0871848.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2243..2434
                     /gene="yaiA"
                     /locus_tag="ACHUPM_004235"
     CDS             2243..2434
                     /gene="yaiA"
                     /locus_tag="ACHUPM_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR0871849.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2692..3369
                     /locus_tag="ACHUPM_004236"
     CDS             2692..3369
                     /locus_tag="ACHUPM_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR0871850.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3441..3725
                     /gene="ppnP"
                     /locus_tag="ACHUPM_004237"
     CDS             3441..3725
                     /gene="ppnP"
                     /locus_tag="ACHUPM_004237"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR0871851.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          904 c          990 g          953 t
ORIGIN      
        1 tcgtgagtgg ttgaaatcgg cagatttggc gctttacaaa gcaaagaaag ccggacgtaa
       61 ccgcaccgaa gtggcggcct gacgtccggc gaaagtcatc aggatttgct gagtttttct
      121 gatttttcca tacacttcgt catcgcttcg atcactgcgg cacggaagcc tttctcttcc
      181 agtacgcgta ccgcttcaat ggtggtgcct cccggtgagc agaccatatc tttcagtgcc
      241 cccggatgtt ctcccgtttc cagcaccatt tttgcggaac ccattaccgc ctgagcggca
      301 aatttatacg cctgggcgcg tggcatcccg cccagcacgg cggcgtcggc catcgcttcg
      361 ataaacataa atacgtaggc tggcgaagaa ccgctcacac cgaccaccgg gtggatcatc
      421 ggctcagcaa ttacttccgc ttcgccaaag cagcggaaaa tattcagcac atcagcggta
      481 tcttcggggg ttaccagcgc gtttggcgtt acggaggtca tcccggcatt aaccagcgcg
      541 ggagtgttcg gcatggcgcg gataattttc cggtcatggc ccagcgcgcg ggcaagctgg
      601 tcgagcgtga cacctgcagc aatagaaacg accagagagt ctttattcag gctggaggtg
      661 atttcgctaa gcactttaat catgatgcca ggtttaacgg cggcaaaaat gatgtcggcg
      721 atttgcgcca cttcttgcgc cgattctgcg gcgttgatgc cgaactggtc atgcagggcg
      781 gcgactttat ccggggaggg ggtgtatacc cagatttgcc ctggaagcac ctgaccgctg
      841 gcaatcagac cgccgagaat ggcttttccc atattgccgc agccaataaa accgattttc
      901 ttttccattg cctcactcct gccgtgaaat tcattgtttt gataatcgct ggcagaagca
      961 taaacagaac tatgccggaa ggcaaaagcg cgacacaata gaggattacc caacaaagga
     1021 tgactttatg acaatttggg tggatgccga cgcgtgtccc aatgtaatta aagagatttt
     1081 gtatcgcgcg gcggaacgta tgcagatgcc gctggtactg gtagcaaacc agagtttacg
     1141 cgtgccgcca tcgcgattta ttcgtacgct gcgcgtcgcg gcaggtttcg acgttgccga
     1201 taacgaaatt gtccggcagt gtgaagcggg cgatttggtg atcaccgcag atataccttt
     1261 ggctgctgaa gccatcgaga aaggcgcagc ggcgcttaat ccgcgcggcg aacgttacac
     1321 gccagcgacc attcgtgagc gcctgacgat gcgcgatttt atggatacct tacgtgccag
     1381 tgggatccag accggcggac cagatagcct ttcacaacgt gaccgccagg cctttgccgc
     1441 ggagctggag aagtggtggc tggaagtgca acgtagtcgt ggctaaatgt aatttattat
     1501 ttacacttca ttcttgaata tttattggta tagtaaggga tgtattgaga ttttcacttt
     1561 aagtggaatt ttttctttac aatcgaaatt gtactagttt gatggtatga tcgctattct
     1621 catgacaccg gctttcgccg cattgcgacc tattggggaa aacccacgat gacacaacct
     1681 ctttttctga tcgggcctcg gggctgtggt aaaacaacgg tcggaatggc ccttgccgat
     1741 tcgcttaacc gtcggtttgt cgataccgat cagtggttgc aatcacagct caatatgacg
     1801 gtcgcggaga tcgtcgaaag ggaagagtgg gcgggatttc gcgccagaga aacggcggcg
     1861 ctggaagcgg taactgcgcc atccaccgtt atcgctacag gcggcggcat tattctgacg
     1921 gaatttaatc gtcacttcat gcaaaataac gggatcgtgg tttatttgtg tgcgccagta
     1981 tcagtcctgg ttaaccgact gcaagctgca ccggaagaag atttacggcc aaccttaacg
     2041 ggaaaaccgc tgagcgaaga agttcaggaa gtgctggaag aacgcgatgc gctatatcgc
     2101 gaagttgcgc atattatcat cgacgcaaca aacgaaccca gccaggtgat ttctgaaatt
     2161 cgtagcgccc tggcacagac gatcaattgt tgattttaga gcgcctatac ttaacgttca
     2221 tcccgtgaaa taaggaagaa cgatgccaac gaaaccgcct tatcctcgtg aagcatatat
     2281 agtgacgatt gaaaaaggaa agccaggaca gacggtaacc tggtaccaac tcagagccga
     2341 tcatcctaaa ccagactcgt tgatcagtga acatccgacg gctcaggaag cgatggatgc
     2401 gaaaaaacgc tatgaggacc ctgacaaaga gtgaccgcat cagactgctc ggaagggatt
     2461 ctgagtgcca ctacaaggga tctgcgtcac atttttcata attcatgttt ttctaataat
     2521 tagaatatta aacaataaca atccattact ggaatcattt ggaatcttta cattatgccg
     2581 tgcacgtctg ctgctacgct ttttgtcatt tgtagcacaa gtaagtgtca gcagtggtgc
     2641 tccacacttg cccggtaatt aacgacgaaa gaaaagtaag gtggatgaac aatgagtgcg
     2701 tcgttggcga tcctgaccat cggcattgta cctatgcagg aggttttgcc gctcctgacg
     2761 gaatacattg acgaagataa tatttcccat catagcctgc tggggaagtt aagtcgtgaa
     2821 gaagtgatgg cggagtacgc gccagaagca ggcgaagaca ccattctcac attattaaat
     2881 gacaaccagc tggcccatgt ttcgcgtcgt aaagtggagc gagacctgca aggtgtggtt
     2941 gaagtgctcg ataatcaggg ttatgacgtc attttattaa tgagtacagc aaacattagt
     3001 agtatgactg cgcgtaatac gatctttctt gagccgtcgc gaatattgcc tccactggtt
     3061 tcctctattg ttgaagatca tcaggtgggg gttatcgttc cggttgagga gatgctgacc
     3121 gttcaggcgc aaaaatggca aattttgcag aaatcgccgg tattttcatt gggtaacccc
     3181 attcatgatt cagaacaaaa aatcattgat gccgggaaag aattactggc aaaaggggct
     3241 gatgtcatca tgctggattg tttaggattt caccaacgtc atcgcgattt actgcaaaaa
     3301 cagctcgatg ttcctgtctt gctgtctaac gtattgattg cacggctggc tgcggaatta
     3361 ctggtgtaat tttgcgtgac agccagcgcc tctggcccct atagtgaagt agatgttcaa
     3421 ctaccagaca gggccagttt atgcttcaaa gtaatgagta cttttccggc aaagtgaaat
     3481 caatcggctt ttccagcagc agcactggtc gcgccagcgt gggtgttatg gttgaaggcg
     3541 aatacacctt cagcaccgct gagccggaag agatgacggt aatcagtggc gcgctgaatg
     3601 tgttactgcc tgacgcgacc gactggcagg tgtatgaagc cggttcggtg tttaatgttc
     3661 ccggtcacag tgagtttcat ctgcaagttg ccgaacccac ctcttatctg tgccgctatc
     3721 tgtaattcct cgccttcccc ttgaacggga gggcattttt ctgaaatatc ctctctttaa
     3781 cccataataa tatttcct
//
LOCUS       DAZPAC010000323         3745 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZPAC010000323 DAZPAC010000000
VERSION     DAZPAC010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.326"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            572..1561
                     /gene="araF"
                     /locus_tag="ACHUPM_004238"
     CDS             572..1561
                     /gene="araF"
                     /locus_tag="ACHUPM_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFR0871852.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1631..3145
                     /gene="araG"
                     /locus_tag="ACHUPM_004239"
     CDS             1631..3145
                     /gene="araG"
                     /locus_tag="ACHUPM_004239"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR0871853.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3160..>3745
                     /gene="araH"
                     /locus_tag="ACHUPM_004240"
     CDS             3160..>3745
                     /gene="araH"
                     /locus_tag="ACHUPM_004240"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR0871854.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTT"
BASE COUNT          928 a          848 c          996 g          973 t
ORIGIN      
        1 gaattttaat gatagtgctt tttgtatctt gttgatatta atcaatgaat ttgaattcat
       61 gatgaagtga tgattttata aaacgttttt tcatttttgc gagatggctc tcattatacg
      121 tgttctgtta attaatgagc acagtgataa ttaatggcga aatgaatgac gtgcattttc
      181 cacatctttg agttgcggtt attacaccat ttcaaaaact caacgccagg taatgcggcc
      241 tattgactgg ttaaaaagaa gacatcccgc atgggtacca aagacaacaa ggatttccag
      301 gctaatctta tggattaatc tgctgtgcat tcgacaattt gtctgacaaa ttggctttcc
      361 cttatgtctt ttcccgctaa atttatgcac gttctcactg taattctgcg atgtgatatt
      421 gctctcctat ggagaattaa tttctcgcta aaactatgtc aacacggtca cttatctttt
      481 agataaaagg taatgctttg ttttccgatt aatttaacga aagtcattcg tttttgccct
      541 acacaaaacg acactaaagc tggagagaac catgcacaaa tttactaaag ccctggcagc
      601 cattggtctg gcagccgtta tgtcacaatc cgctatggcg gagaacctga agctcggttt
      661 tctggtgaag caaccggaag agccgtggtt ccagaccgaa tggaagtttg ccgataaagc
      721 cgggaaggat ttagggtttg aggttattaa gattgccgtg ccggatggcg aaaaaacatt
      781 gaacgcgatc gacagcctgg ctgccagtgg cgcaaaaggt ttcgttattt gtactccgga
      841 ccccaaactc ggctctgcca tcgtcgcgaa agcgcgtggc tacgatatga aagtcattgc
      901 cgtggatgac cagtttgtta acgccaaagg taagccaatg gataccgttc cgctggtgat
      961 gatggcggcg actaaaattg gcgaacgtca gggtcaggaa ctgtataaag agatgcagaa
     1021 acgtggctgg gatgtcaaag aaagcgcggt gatggcgatt accgccaacg aactggatac
     1081 ggcacgtcgc cgtacaacgg gttctatgga tgcgctgaaa gcggccggat tcccggaaaa
     1141 acaaatttat caggtaccta ccaaatctaa cgacatcccg ggggcatttg acgctgccaa
     1201 ctcaatgctg gttcaacatc cggaagttaa acattggctg atcgtcggta tgaacgacag
     1261 caccgtgctg ggcggcgtac gcgcgacgga aggtcagggc tttaaagctg ccgatatcat
     1321 cggcattggc attaacggtg tggatgcgtt gagcgaactg tctaaagcac aggcaaccgg
     1381 cttctacggt tccctgctgc caagcccgga cgtacatggc tataaatcca gcgaaatgct
     1441 ttacaactgg gtagcaaaag acgttgaacc gccaaaattt accgaagtta ccgacgtggt
     1501 gctgatcacg cgtgacaact ttaaagaaga actggagaaa aaaggtttag gcggtaagta
     1561 atttgccgga aaaattcccc tctgcatgat gcagaggggg tgtgaacgac cagtgattca
     1621 cggagacgtt atgcaacagt ctaccccgta tctctcattt cgcggcatcg gtaaaacgtt
     1681 tcccggcgtt aaggcgctga cggatattag ttttgactgc tatgccggtc aggttcatgc
     1741 gttgatgggt gaaaatggcg caggaaaatc aactctctta aaaatcctca gcggcaacta
     1801 tgcgccaacc acgggttctg tagtgattaa tgggcaggaa atgtcctttt ccgacacgac
     1861 cgcagcactt aacgcgggtg tggcgattat ttaccaggaa ctgcatctcg tgccggaaat
     1921 gaccgtcgcg gaaaacatct atctcggcca gctgccgcat aaaggcggca ttgtgaatcg
     1981 ctcattgctg aattatgagg cgggtttaca acttaaacat cttggtatgg atattgaccc
     2041 ggacacgccg ctgaaatatc tctccattgg tcagtggcag atggttgaaa tcgccaaggc
     2101 gctggcgcgt aacgctaaaa ttatcgcctt tgatgagcca accagctccc tttctgcccg
     2161 cgaaatcgac aatcttttcc gcgttattcg tgaactgcga aaagaggggc gggtgatctt
     2221 atacgtttct caccgtatgg aagaaatatt tgccctcagc gatgccatca ctgtctttaa
     2281 agatggacgt tatgtcaaaa cctttaccga tatgcagcag gttgaccacg acgcgctggt
     2341 gcaggcgatg gtcgggcgcg acattggcga tatctacggc tggcaacctc gtagttatgg
     2401 cgaggagcgc ctgcgtcttg atgctgtgaa agcaccaggc gtgcgtacgc caataagtct
     2461 ggcggttcgc agtggtgaaa ttgttgggct gtttggtctg gtaggggcgg ggcgtagcga
     2521 attaatgaaa ggcttgtttg gcgggacgca aatcaccgcc ggtcaggttt atatcgacca
     2581 acagccgatc gatattcgta aaccgagcca cgccattgcc gcaggcatga tgctctgccc
     2641 ggaagatcgc aaagcggaag gcattattcc cgtgcactcc gttcgcgaca atatcaacat
     2701 cagtgccaga cgcaaacatg tgctcggcgg ttgtgtaatc aacaacggtt gggaagaaaa
     2761 caatgccgat cagcacattc gttcgctcaa catcaaaacg ccgggcgctg agcaactgat
     2821 catgaatctc tcaggcggaa atcagcaaaa agccattctg ggccgctggt tatcggaaga
     2881 gatgaaggtc attttgctgg atgaacctac gcgcagcatt gatgttggtg ctaagcacga
     2941 aatatataac gtaatttatg cgctggcggc gcagggtgtg gcggtgctgt ttgcctccag
     3001 cgacttacct gaagtcctcg gcgttgccga ccggattgtg gtgatgcggg aaggtgaaat
     3061 cgccggtgaa ttgttacacg agcaggcaga tgagcgtcag gcactgagcc ttgcgatgcc
     3121 taaagtcagc caggcagttg cctgagtaag gagagtatga tgtcttctgt ttctacatcg
     3181 ggttctggcg cacctaagtc gtcattcagc ttcgggcgta tctgggatca gtacggcatg
     3241 ctggtggtgt ttgcggtgct ctttatcgcc tgtgccattt ttgtcccaaa ttttgccacc
     3301 ttcattaata tgaaagggtt gggcctggca atttccatgt cggggatggt ggcgtgtggc
     3361 atgttgttct gccttgcttc cggtgacttt gacctttctg tcgcctccgt aattgcctgt
     3421 gcgggtgtca ccacggcggt ggttatcaac ctgactgaaa gcctgtggat tggcgtggca
     3481 gcggggttgc tgcttggcat tctctgtggc ctggtcaatg gctttgttat cgccaaactg
     3541 aaaataaatg ctctgatcac aacactggca acgatgcaga ttgttcgagg tctggcgtac
     3601 atcatttcag acggtaaagc ggtcggtatc gaagatgaaa gcttctttgc ccttggttac
     3661 gctaactggt tcggtctgcc tgcgccaatc tggctcaccg tcgcgtgtct gattatcttt
     3721 ggtttgttgc tgaataaaac cacct
//
LOCUS       DAZPAC010000324         3735 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZPAC010000324 DAZPAC010000000
VERSION     DAZPAC010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.327"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..182)
                     /locus_tag="ACHUPM_004241"
     CDS             complement(<1..182)
                     /locus_tag="ACHUPM_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFR0871855.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            426..683
                     /locus_tag="ACHUPM_004242"
     CDS             426..683
                     /locus_tag="ACHUPM_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFR0871856.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(733..1683)
                     /gene="uspE"
                     /locus_tag="ACHUPM_004243"
     CDS             complement(733..1683)
                     /gene="uspE"
                     /locus_tag="ACHUPM_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR0871857.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="ACHUPM_004244"
     CDS             complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="ACHUPM_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR0871858.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="ACHUPM_004245"
     CDS             complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="ACHUPM_004245"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR0871859.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3308..>3735)
                     /locus_tag="ACHUPM_004246"
     CDS             complement(3308..>3735)
                     /locus_tag="ACHUPM_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR0871860.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1003 a          886 c          893 g          953 t
ORIGIN      
        1 ataatgctac caacagcact gataattatg gtgtagatta aaaattgcac cgcatggaat
       61 aaaaaacctt ttcttttacg atttccgcgg cgaaaccaaa agctcatcag aatcaatgct
      121 gcgcagctac cgaaaataat gaccagatta agcgcattat ttgtaaacag ttcagcgatc
      181 attgttttat caggctcctc cagataattg tcgtcatgcc ggaaacccct ggcggggcta
      241 ttttaccgcg acaattcatt cagatcatca atagtcaggg aaggaagtag caacattagc
      301 taactaatca atgcgttgat tgtaaatcca gctaagaggt gaggttttca gagcagacaa
      361 cggtgaaatg tcatggtatt gttacgttta ggtaacaaga aatttgtctg cacaaggatt
      421 acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa aaaatttctc tttggtttgt
      481 tggcggtttt cattattgcg gcgtcggttg tgactcgcgc gactatcggc ggcgttatag
      541 aacagtacaa tattccgctg tctgagtgga cgacatcaat gtatgtgatt cagtcatcga
      601 tgatttttgt ttatagcctg gtctttactg tgttgctggc aatcccgttg ggaatttatt
      661 tccttggcgg cgaagagcag taagtaaaaa ataggcccga taactcgggc cttgtcagtt
      721 attgaagagt cgttaatcgt cttcttcgtc atccagttca acgggtgtct gatactggtc
      781 aggtttaatg accaacagat cgcagcgaag atgatcaatc acctgttccg ccgtgttgcc
      841 gaggaatgct gctgaaatac cggtgcgtcc taccgtgccc agaaccacaa tccccgcctg
      901 taagtgctcc gccaaatcag gaataacctc ttccggcaga cctttttcta cgtgcgtcat
      961 gttttcatta atgccgaatt tctgccgcag agctttcatt gccagcaaat gttgcccacg
     1021 aatggcatcg ttataaacgc tcggatcaaa ttccggcagt tcaatcgcga tattaattgg
     1081 cgttactggg taagcgccaa ccagatgaac ttcggtatgg ttgacttgtt ctgccagttc
     1141 gatcgtctct ttgaccagtt tttcattgag cgcattatga tacggctctt cactggcgag
     1201 attcaccgcc accagcgcct tacctccttc cggccacggc tggtctttca ccatccacac
     1261 cgggcttggg catttgcgta acagatgcca gtccgttggc gtaaaaatca ccgcttccag
     1321 acggtcatgt tggtgcgcca tttttagcac caaatcgtgc ccgccgctga tcacttcctg
     1381 aatgatggct tcgaagggac ggttatgcca gaccacttta atttcaatgg gaacgccagc
     1441 attgagataa tattttgcct gctcgtggat ccaggctgta cgctggctga tgacgccctg
     1501 acgcatagcg gtacgttcgt ccggggagag cagggtggtc atttcgtatg agaagtcata
     1561 gatcggcaaa aaggctttaa ttttgccacc aatccgttga tgtaaataaa cagctcgccg
     1621 caatgctggt tggtcgtcct ggttaggatc gataacaacg agcatgttct gatacatagc
     1681 catacagggt ctccttacaa caactgtcaa cgcagtttgt aattaaaaga ttaacccata
     1741 tctggtgaat gaaacagtga tgaaccttct gccagatcaa taaatcagaa aaatttaatg
     1801 atatgacaga aggatagtga gttatgcgga aaaatcaggc aacattacgc gtatgaccag
     1861 caagctgggc cagcgcatcg ttattttcga tggtgatgta tttacctttg actgccagca
     1921 taccgctttt ctggaagcga cctagcagac ggctgatggt ttctaccgtc aggcccagat
     1981 agttaccgat atcgccacga gtcatcgtca ggcggaattc acgaggggag aagccgcgtt
     2041 gggcaaaacg acgggacagg ttgtagatga atgcagccag acgttcctcg gcatttttct
     2101 tcgacaacag caagatcatg tcctgatcgc ctttgatttc accgctcatc agacgcatca
     2161 tctgctgacg cagattcggc attttaccgg acaaatcgtc cagcgtttcg aacgggattt
     2221 cacataccat cgaggtttcc agcgcctgag cgaagctcgg gtgatggccg ctgccgatgg
     2281 cgtcaaatcc caccaggtcg ccagctaaat ggaaaccagt gatttgctcg tcgccttgct
     2341 cagtgatggt ataactttta atcgtaccgg agcggatggc ataaagcgat ttaagttcat
     2401 caccagcctt aaacagcgtc tggcctttct gaataggctt cttccgctca atgatattat
     2461 caagctgatc aagctcatgt tcgttgagtg tgaacgggat gcaaagctgg ctgatgctgc
     2521 aatcctggca atggatagca caaccgccag actgaatgcg ccgtataatt cgcttttccg
     2581 ggatcatagg tctgctcaag ccgtaattga tatttgtcaa ttttaacatc tttttaggga
     2641 gcaagtaagt ctaagcaaac cttaacagca gagaattccg atattagatg taaatatatg
     2701 tctatctatt tgaaaaccct taagttgtta agggtaactt tacataaaag tgtgaacaag
     2761 ctggcacaaa ttgtttaatg tttacagcaa aagataacct tcgtggcgca ataaccactc
     2821 ttttcgctga actccgcctg catatccggt catggtgccg tttcggccaa taacccgatg
     2881 gcaaggtacg acgatgctga tgggattcga tccgtttgcc gcaccaacgg cacgcgccgc
     2941 gccagggcgg cccaattgtt cagccagttg gccgtaatgc attacctgtc cgcaggggat
     3001 agtgcgtaga gttttccaga cttcgcgctg aaatggcgtc ccccctgtgg cagtgggaag
     3061 cgtatcaata atgctaagat taccggcaaa ataatcacga agcttgtcgc ttaaaccgcc
     3121 tgggttggtg gcagaaatgc gctcatagcc ttctttgcga taatggatgt ccagcagctg
     3181 caccatgcgt tcgctgtact cttcccattc aaccgcccgc aggcgaaatt gctcatcgca
     3241 aatcacccac agtggaccca gtggcgtggc aattttttct tcaagtaatc tcagcatccg
     3301 ttctctctta agacaaacgt gggtaaatcc ccggacctat cggcagacca acaagatacc
     3361 acgccagcaa catcagcagc cataccacca aaaagataag cggatagggc aagactaacg
     3421 aatagtaagt acccagtttc gcgtctggtt tgtagcgttg caggaatcca agaaacagtg
     3481 gaacaaaagg agataccggc gctaaaggca atacggatga gtcggcaata cgaaagagga
     3541 tttgcgcaaa tgccgggtga aagccaagta gcataaacat tggtacgaaa atgggggcca
     3601 gaatcgacca gattgcggaa ccgctggcaa taaacatgca taagaaagag gaaagcaacg
     3661 ccagaccgac aaacgccggg atgccgctaa gccctgaact ttccagtata tcggtcagcc
     3721 ccacggcgat gaatt
//
LOCUS       DAZPAC010000325         3726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZPAC010000325 DAZPAC010000000
VERSION     DAZPAC010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.328"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            321..437
                     /locus_tag="ACHUPM_004247"
                     /pseudo
     CDS             321..437
                     /locus_tag="ACHUPM_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            812..1054
                     /gene="yiiF"
                     /locus_tag="ACHUPM_004248"
     CDS             812..1054
                     /gene="yiiF"
                     /locus_tag="ACHUPM_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR0871861.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="ACHUPM_004249"
     CDS             complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="ACHUPM_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR0871862.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="ACHUPM_004250"
     CDS             complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="ACHUPM_004250"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0871863.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2942..>3726)
                     /gene="fdoH"
                     /locus_tag="ACHUPM_004251"
     CDS             complement(2942..>3726)
                     /gene="fdoH"
                     /locus_tag="ACHUPM_004251"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR0871864.1"
                     /translation="GCKACQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSE
                     VEQNDKLEWLIRKDGCMHCSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAG
                     CPFDIPRLNPEDNRVYKCTLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERV
                     AELKTRGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWK
                     PLAAVGFAATFAASIFHYVGVGPNRADEEENNLHEEKDEERK"
BASE COUNT          948 a          942 c          896 g          940 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcagta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat
     1321 ggtgtcgtca caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag
     1381 agattactct ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc
     1441 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
     1501 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
     1561 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca
     1621 gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta
     1681 acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag agccacatac
     1741 cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc
     1801 catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1861 ggacgagaaa tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc
     1921 cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc
     1981 aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     2041 cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     2101 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     2161 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     2221 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     2281 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     2341 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc
     2401 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     2461 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     2521 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc
     2581 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     2641 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     2701 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2761 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2821 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2881 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2941 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     3001 tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag
     3061 ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     3121 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg
     3181 taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct
     3241 cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     3301 ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca
     3361 gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     3421 ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg
     3481 catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     3541 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     3601 agcgcatcac cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt
     3661 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3721 agccga
//
LOCUS       DAZPAC010000326         3702 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZPAC010000326 DAZPAC010000000
VERSION     DAZPAC010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.329"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            235..1581
                     /gene="yagA"
                     /locus_tag="ACHUPM_004252"
     CDS             235..1581
                     /gene="yagA"
                     /locus_tag="ACHUPM_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HFR0871865.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKRPATMAQHNLA
                     NDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGERSSVRKKRPRLG
                     RLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRALSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFKKHMYGVFMHLRY"
     gene            2466..>3702
                     /locus_tag="ACHUPM_004253"
     CDS             2466..>3702
                     /locus_tag="ACHUPM_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
                     /protein_id="HFR0871866.1"
                     /translation="MLAEYERRKGILDTRLSELEKNGGAALAVLDAQQARLLGQQTRN
                     DRAISEARNKLSSVTESLNTARNALTRAEQQLTQQKNTPDGKTIVSPEKFPGRSSTNH
                     SIVVSGDPRFAGTIKITTSAVIDNRANLNYLLTHSGLDYKRNILNDRNPVVTEDVEGD
                     KKIYNAEVAEWDKLRQRLLDARNKITSAESAVNSARNNLSARTNEQKHANDALNALLK
                     EKENIRNQLAGINQKIAEEKRKQDELKATKDAINFTTEFLKSVSEKYGAKAEQLAREM
                     AGQAKGKKIRNVEEALKTYEKYRADINKKINAKDRAAIAAALESVKLSDISSNLNRFS
                     RGLGYAGKFTSLADWITEFGKAVRTENWRPLFVKTEAIIAGNAATALVALVFSILTGS
                     ALGIIGYGLLMAVTGALIDE"
BASE COUNT         1082 a          791 c          920 g          909 t
ORIGIN      
        1 gtagtaacag ttatggcgtg acagtcgaat tgatattcta ctgaaccctg acagaaacat
       61 ggttgatata ttctctcact ttaatgttgt catgatataa ataaaaacaa ttcatttgaa
      121 aggatatcag aagaagatat cggggcgcac agttcatctc tgcctttgtt tcatgggggg
      181 ttctttgtat attttactta ctgtttaagc atgtacataa ggagtgcttt cattatgcca
      241 atgatacttg taaagaagaa tccccggggc aaagtgattc gggagcttag cagtgaggaa
      301 gagactgcag taaagacggt ctgtggactg aaaaggccag caaccatggc acaacataac
      361 ctggccaatg atcttctccg ggaaatgcgt gagtatgatg catggttaca gtgtgactgt
      421 attccgggag attcgccggc catgaatttt gctgctctta aaaacaatac gggtactctt
      481 tatctgtcca gttttaatca tgagcatgca cctgaatgcc ccatgtatcg tcagttatcc
      541 ggaaatgaag aggaaacatc cttcggagca agccggcatc ccgttagcac ccgtataaat
      601 tacagaaact ttttaccacc tgatgacagt aattctgtta tcagactgca ggcccgctct
      661 gcttaccata atggtgagcg ctcctccgtt cgcaagaaac gtccgcgcct tggccgttta
      721 ctcctgtcgc ttattgaaga tgcagggctg aataaactgg attccctggc taatccccgt
      781 atccggacta attatcgtga gtgtcttgat gccatacggc aggttacact acagcaggag
      841 tatattcggg gaagagcctt atctgaaatt atccatttcc ggccaggaat gtctgaaagg
      901 tctcaggagc ggttaatgga aacgctggag aattcagaac gacactggcc tgccagaaga
      961 aagcatatgt tctttcagat ttttatggcg cagcacatct gtcgtgatgc tgtggaaatt
     1021 cactgggcga acggtaatat tcaggtcatc agacctgtgc gggggatcag tattaatggt
     1081 gaagcgcagg gcggaatacg tcccccttac tgggttattc ttgctttttg ccggagtgcc
     1141 gatggcagaa tcatctgcag tgaagggtat gctcatgctc tttatcaact gacatgtccg
     1201 gtgcctgtgg acagcaaact ggaacgaaac acgctcactg ctcttctgaa tgtggccagc
     1261 tggcttaaaa gaaagccagg tacgccggaa ttaagtctgg aaaggcccct gtttgataca
     1321 gaagtttatg ttaatggtga aaagaaatat gtactgccgg atttcattgt cacagcaagg
     1381 gctcctgacg gaaagacggc cagagtggtc atcgaaacga tgggatatga agacagtgat
     1441 tactgcgcga gaaaatccag gcagcatacc ggcatgaagc agattggtgt tctgcatacc
     1501 gatccaccga aatggctgga taacgatcat ccccctttta agaaacatat gtacggtgtt
     1561 tttatgcatc tcaggtactg agatattttg tggctcagtt ctgtaacttt tcccgtaaca
     1621 ttgtctgttg ttacgggaaa gtccggtttt tgtattgcac cagagaatac ccagactgtg
     1681 atgctgccac agcgtcagca ggctttctga acggtgtaac atcacttcat gttaatgata
     1741 atcactatca tcaaatcttg acatgccatt ttctccttaa taaattaata ctgtatatgt
     1801 atccatatac gtaagcagtt aattcatttg ttttcctcag aggatgaagg agataccgaa
     1861 tgtctgaccc tgtacgtatt acaaatcccg gtgcagaatc gctggggtat gattcagatg
     1921 gccatgaaat tatggccgtt gatatttatg taaaccctcc acgtgtcgat gtctttcatg
     1981 gtaccccgcc tgcatggagt tccttcggga acaaaaccat ctggggcgga aacgagtggg
     2041 tcgatgattc cccaacccga agtgatatcg aaaaaaggga caaggaaatc gcagcgtaca
     2101 aaaacacgct cagcgcgcag cagaaagaaa atgagaataa gcgcactgaa gccggaaaac
     2161 gcctctctgc ggcgattgct gcaagggaaa aagatgaaaa cacactgaaa acactccgtg
     2221 ccggaaacgc agatgccgct gatattacac gacaggagtt cagactcctg caggcagagc
     2281 tgagagaata cggattccgt actgaaatcg ccggatatga cgccctccgg ctgcatacag
     2341 agagccggat gctgtttgct gatgctgatt ctcttcgtat atctccccgg gaggccaggt
     2401 cgttaatcga acaggctgaa aaacggcaga aggatgcgca gaacgcagac aagaaggccg
     2461 ctgatatgct tgctgaatac gagcgcagaa aaggtattct ggacacgcgg ttgtcagagc
     2521 tggaaaaaaa tggcggggca gcccttgccg ttcttgatgc acaacaggcc cgtctgctcg
     2581 ggcagcagac acggaatgac agggccattt cagaggcccg gaataaactc agttcagtga
     2641 cggaatcgct taacacggcc cgtaatgcat taaccagagc tgaacaacag ctgacgcaac
     2701 agaaaaacac gcctgacggc aaaacgatag tttcccctga aaaattcccg gggcgttcat
     2761 caacaaatca ttctattgtt gtgagcggtg atccgagatt tgccggtacg ataaaaatca
     2821 caaccagcgc agtcatcgat aaccgtgcaa acctgaatta tcttctgacc cattccggtc
     2881 tggattataa acgcaatatt ctgaatgacc ggaatccggt ggtgacagag gatgtggaag
     2941 gtgacaagaa aatttataat gctgaagttg ctgaatggga taagttacgg caacgattgc
     3001 ttgatgccag aaataaaatc acctctgctg aatctgcggt aaattcggcg agaaataacc
     3061 tcagtgccag aacaaatgag caaaagcatg caaatgacgc tcttaatgcc ctgttgaagg
     3121 aaaaagagaa tatccgtaac cagcttgccg gcatcaatca gaagatagcg gaagagaaaa
     3181 gaaaacagga tgaactgaag gcaacgaaag acgcaattaa tttcacaaca gagttcctga
     3241 aatcagtttc agaaaaatat ggtgcaaaag ctgagcagtt agccagagag atggccgggc
     3301 aggctaaagg gaagaaaata cgtaatgttg aagaggcatt aaaaacgtat gaaaagtacc
     3361 gggctgacat taacaaaaaa attaatgcaa aagatcgtgc agcgattgcc gcagcccttg
     3421 agtctgtgaa gctgtctgat atatcgtcta atctgaacag attcagtcgg ggactgggat
     3481 atgcaggaaa atttacaagt cttgctgact ggatcactga gtttggtaag gctgtccgga
     3541 cagagaactg gcgtcctctt tttgttaaaa cagaagccat tatagcaggc aatgccgcaa
     3601 cggctcttgt tgcactggtc ttcagtattc ttaccggaag cgctttaggc attatcgggt
     3661 atggtttact gatggctgtc accggtgcgc tgattgatga at
//
LOCUS       DAZPAC010000327         3697 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZPAC010000327 DAZPAC010000000
VERSION     DAZPAC010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.330"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            247..1116
                     /locus_tag="ACHUPM_004254"
     CDS             247..1116
                     /locus_tag="ACHUPM_004254"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR0871867.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(1276..1869)
                     /gene="rclC"
                     /locus_tag="ACHUPM_004255"
     CDS             complement(1276..1869)
                     /gene="rclC"
                     /locus_tag="ACHUPM_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFR0871868.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(1881..2117)
                     /gene="rclB"
                     /locus_tag="ACHUPM_004256"
     CDS             complement(1881..2117)
                     /gene="rclB"
                     /locus_tag="ACHUPM_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFR0871869.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(2226..3551)
                     /gene="rclA"
                     /locus_tag="ACHUPM_004257"
     CDS             complement(2226..3551)
                     /gene="rclA"
                     /locus_tag="ACHUPM_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFR0871870.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         1058 a          797 c          778 g         1064 t
ORIGIN      
        1 cattctccat taattcttcg tgatatcgca cattttcgtt atgcccaagg ccctaagtgt
       61 atcaaaaaca gcaaggtcga aggcaaaagc actggtagcg tctgcttcgt tgtccccgaa
      121 aatagagagg tttcagtcct acattattaa tgaatttttt gcataagtga tatcaaaatc
      181 cacatactaa tttgaggtta tgttttaacg tagactcatt gttcatgcct aatggaggga
      241 ctgacagtgg aattttccgt attaagtaac aatctgaaaa tgccgatgat gggatttggt
      301 gtttttcagg ttaccgataa aaacgtgtgc aaacagtcag tgttgaacgc tatccgcacg
      361 ggttatcgac tcatcgacac tgcagcggta tacggtaatg aagatgccgt cggtgaagcc
      421 gttcgtgaag ctatatctga aggtttatgt acccgagatg agttatttat aacgtctaaa
      481 ttgtgggggc aggatatgtt gaatcaggat acggcagcag caggtattga agcatcatta
      541 aaaaaatcgg gactagagta cttcgacctc tatttattgc accaggctat gcgcgattat
      601 ttcagtgtgt ggcgtgcact cgaagatgcc tatgaagagg gcaaattaaa agcaattggg
      661 gtttccaatt tctatcctca tgttctggcg aacttttgtg aaacggtaag agttaaaccg
      721 atggtcaacc aggtcgagtt gcatccctat tttgcccaac cagaggcgct ggcaaccatg
      781 aagtattata acgtgcagcc tgaagcatgg gctccgttag gtggtggacg acataaaccc
      841 tttgaaaata atctgcttca gagtattgca gatgcccatc aaaaatcgat ttctcaagtc
      901 attctgcgtt ggaatattca acggggagtg gtcgttattc cgaaatcgac acatcaacag
      961 cgtatcgaag aaaattttgc tatctgggat ttctcactga cagagaaaga aatggcacaa
     1021 attagttcgc ttgatttggg ttatgttggg gagtcggtaa aacattttaa tcctgaattt
     1081 gttcgtggtt gtcttgctgt aaaaatacat gattgatatt aatcatatat tttacctgag
     1141 acgacaagaa tcttttaaca ggggagtgat attgatcttc actctgtcat atctccggta
     1201 atatggcgtc aggcttcatc atcagaaagg gggcctgacg cctgaaaaag tgaacaacag
     1261 acagtgttcg gattatcaat attcagtttt taacgttgaa ctggattcat tactgcgttg
     1321 tttaagaatt tcccgcgccg aatctgccat tatcatgaca gcacctgcca gcatcagagt
     1381 atctttcaat accaagcgac cagcaccgga taaataaggg aaaccatgat gagcgtcacc
     1441 caatgcgggt acccatgcct ccggggtggt gattaaaaat gagagtgtta ccagcggcgt
     1501 ggtatatgcc atcagtcctc ccaataaacc taaccagcga ttgacaggat tagccaaaac
     1561 cagcagagca ataatcacct ccacgacgcc aagaccgttg gaaaaaccat aggtattatt
     1621 ggccgattgc catgcccttg cttctggttt gtattcgcct tcgtgagtca gatactgttt
     1681 atagtcttcc gggtgttcat aaaagaacga cattagtgga ctgtttgcga cgaatggtgt
     1741 aatgctgtct gcctcgtaag ggacaaactt taataaccca atccacataa aaacaatcgc
     1801 aatactcaga cgaatcaatg acaggccaat tttatctccc cgacttaaca ggtgcaggta
     1861 tttttccatt gtgaacatcc ttatttatac aatactgctg ttgcggttga attattatca
     1921 attcgcatcg atgtgacttt ccaggatgaa gcattatatt ttctggctat atcagcaatg
     1981 gtatcctcca gaactgtcgg tgatgctgat actgacgaga cgctgatatg tttcagaatt
     2041 attttatcat ctgcattggt ggaaaattcc ataacgccag ataatagtgt tgcaaataaa
     2101 acagattttt taaacatata aaataccatc aatgaagtga ttaagaagtt atagagtaac
     2161 agagaattaa taagattctt ttctctgaga cgccagaata tttgttctgg cgtctgattt
     2221 tgagtttatt tgactaatga aaatagatca ttgagtgatt cgctcatcga cggatgagta
     2281 aatatctgat cgcgtaatat gctataaggc agcccggcat ccatcaccat tttcactata
     2341 ttgatcatct cgtgggagtc aacacacagc agtgatgccc ctaatatacg ttgggtttta
     2401 ttatcaacaa tcgcttttaa taccccacga gtatcattca tcactctggc acgcggaatt
     2461 gcagctacag gcaatgtcac cacctgaata tcagcaccac tctctctggc ctgttcttct
     2521 gtcataccaa ccctggacag tggcggtgtc atgaatacgg aataaggcac atttttccga
     2581 tcatcagtac tacgtctgcc ttctcccagc aactcatcac gcacaatgcg gtaatcatcc
     2641 agtgaaatat aagtaaattg cagcccgccg gtaacatctc ccatcgccca aatattgtct
     2701 gcggtggtat gtaattgctt gtcaacgaca attgccccgc gctcgtttac tgcgataccg
     2761 gcattttctg gatgtaacga agcggtagcc ggttgacgac cggaagctat taacagtgca
     2821 tccaccgcca gttgggcgtg ctcgctatgc acttgcactt gattttcatg gtgactgatt
     2881 cgctccacat gggcattgag gataatatcg acgccctgat cgcgtaaaat cgtcgcgata
     2941 ttatcagcaa tatcccgatc ttcccgaggc aaaaacagcg aagctgcttc taaaatggtt
     3001 accttgctgc caaaattagc gaacatagag gcgaactcaa cgccaatata tccgccgccc
     3061 aaaataccta aatgcccagg caattctttt agattaagta atccggtgct gtcatataca
     3121 ccaggcgtgg tggtaattcc aggaattggc ggaaccacgg tttgtgcacc ggtattaata
     3181 aaaattttct cgccatgaat ttccagattt ccctcaggcc gatgaacacg caggctatga
     3241 ttattgataa actccgcctg gccgtcgatc acgtcgatat tgggcatatc cgcaagatta
     3301 tgaaaattct tattacgtaa aaaattaacc acttcatttt tacgctgtat ggcacggaca
     3361 aaatctgtgt gctgctgtgc gtcatgaacc aatgttttgg ttgggatgca gccgatatta
     3421 atacaggtcc cgccatacat tgcatttgat tgttcgatga gagccactcg ccaacctgct
     3481 tttgccagcg tgacggctaa tgtttttcca gccttgccaa aaccaataat aactgcctga
     3541 tatttattca tgatgatttc ctgtcgaaac acgttcattt gacatcatga ctatagacgc
     3601 aaaagagcaa ataccatttc ttcacaacgc ttgatatttt gtcttatcgt ctggtatttt
     3661 ctttttctta atcagaatca caaaacaaaa atcatga
//
LOCUS       DAZPAC010000328         3663 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZPAC010000328 DAZPAC010000000
VERSION     DAZPAC010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.331"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..461)
                     /locus_tag="ACHUPM_004258"
     CDS             complement(<1..461)
                     /locus_tag="ACHUPM_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR0871871.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHV"
     gene            complement(508..1227)
                     /gene="btsR"
                     /locus_tag="ACHUPM_004259"
                     /gene_synonym="yehT"
     CDS             complement(508..1227)
                     /gene="btsR"
                     /locus_tag="ACHUPM_004259"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR0871872.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1224..2909)
                     /gene="btsS"
                     /locus_tag="ACHUPM_004260"
     CDS             complement(1224..2909)
                     /gene="btsS"
                     /locus_tag="ACHUPM_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR0871873.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3131..3196
                     /locus_tag="ACHUPM_004261"
                     /pseudo
     CDS             3131..3196
                     /locus_tag="ACHUPM_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3424..>3663
                     /locus_tag="ACHUPM_004262"
     CDS             3424..>3663
                     /locus_tag="ACHUPM_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0871874.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWF"
BASE COUNT          978 a          946 c          846 g          893 t
ORIGIN      
        1 ttcacatgct ggcgcgctgc cagtccacgc agaaaataac gtaaaaattg atcgccgcat
       61 tcgcggaagt ttttatgatc cggtgcgcgc atcatcgctg taatttccgg catcgaaacg
      121 cggaactgct gttcggtgag gatcgccaga atgtcatcgg ttttcagcga aaacgcgatg
      181 cgtaattttt tcagcacgat gttgttatta atgcgacgtt ccggctccag tgccggagca
      241 gactcatcct tgccgcgttt ttcataaatc aggccattga ggaatgacga caaaacaatg
      301 tccggacaac gctgaaaacc ctcttcgtct tctttacgta gccagacggc gatctgttcc
      361 gcggtggctt cgacattacc cagcgccaga atacgcacca ggtcattatt attggctttc
      421 aaaatgtagc gcacgctgcg cagaatatcg ttacttagca tgaggccttc aggtgttaat
      481 gaggcaaaaa gccattttag cagtctttta caggccaatc gcctctttta agcttttcag
      541 ataacggcgg ctgaccggca cggttaagcc attacgcaaa atcaactcgg cctggccgtt
      601 atcttccaga cgaatctcct gtaaatgcgc gaggttaacc agatactgac gatggcagcg
      661 cagtagtggt gtacgacttt ccagggtacg taatgtcaat tcggtaaagc cctctttccc
      721 ttcgtggctg gtaacgtaga caccgctcat ccgactgctg acaaatgcca catctttcat
      781 ttgcagcaaa taaatccgac tatgccccgt acaagggata aatttcagcg cctgttgatt
      841 ttccggtaac agcgaaacat cctgcttgct gcgctcctga cgcaatcgcg ccagcgtttt
      901 ctccagtcgc gcttcatcaa ttggcttcag cagataatca aaggcatgtt cttcaaaggc
      961 tttaatagcg tattcgtcaa acgcagtgag aaaaacaata tacgggcgat gttccgggtc
     1021 aagcatcccc accatttcca gaccactgat gcgcggcatc tggatatcga gaaacagcac
     1081 atccgggcgc agtttatgca ccgcgccgat cccttccacg gcgtttgaac actctccaac
     1141 gatttcaata tcgctctgct cctgcaaaaa tacacgcagg ttctcccgtg ctaacggttc
     1201 atcatcgaca attaagactt taatcatgcc tcgtccctcc atggtagtcg taacgttatt
     1261 cgggtgtaac tatcaggctc acaggcgacg cttattccat agtcatcgcc aaaccgttca
     1321 cgtaaacgct tatccaccag attcatcccc agcccactgg cattggttac cggttgatac
     1381 aaaccggcat tgtcttcgat ctccagcatc aaatgttgcc cctcacgtcg ggcgctgatt
     1441 gccactcgcc ctgtatccag cagttgtgat gtcccatgtt taatggcgtt ttccactatc
     1501 ggttgcaggg taaacgcggg caattgctgc tgggataatt cttgcggaat agcaatgttg
     1561 acctgcaacc gcgactggaa gcgcgccttt tcaatttgca gataagcgtt cacatgttca
     1621 atttcgtcgg cgagagtaac aaactccgaa ggccgcttta agtttttgcg gaaaaaagtg
     1681 gaaagatact gcaccagctg gctggcctgt tcgctgtcgc ggcggatcac cgctttaatg
     1741 gtgttaagcg cattaaacaa aaaatgagga ttcacctggg cgtgaagcag tttgatttct
     1801 gactgggtga gcatcgcttt ttgccgctca tattgcccgg caagaatctg cgccgaaagc
     1861 agttgcgcaa tcccctcgcc cagcgtgcgg tttattgaac tgaataaacg gtttttggct
     1921 tcatacaatt tgatggtgcc catcacgcgc tgattttcgc cacgcaacgg aattaccagc
     1981 gtcgacccca gtttgcattg cggatgcaaa gagcaacgat aaggtacttc gttgccatca
     2041 gcgtagacca cttcaccggt ttcaatcgct tttaaggtgt aaggcgaaga aatcggtttg
     2101 ccgggtaaat ggtggtcgtc accaattccg gtaaaggcca gcaatttctc tcgatcggta
     2161 atcgcgactg caccaatatc cagctcctga tacagcacct gagccacttt catgctgttc
     2221 acttcgttaa acccctgtcg caaaatgcct tccgtcgagg ctgccacttt cagcgcagtg
     2281 gcagaaaaag ccgaagtgta tttttcaaac atcgcgcgtt tatcgagcaa tatacgcata
     2341 aacagcgccg cgccgacggt attggtgacc atcattggcg cagcaatatt actcaccaga
     2401 cgcaccgcat cttcataagg tcgggcgatc gccaggatga tcagcatttg caccatttca
     2461 gcgacgaacg tgacggcacc ggcggtaatg gggttaaaga ctttatcagt gcgcccgcga
     2521 cggatcagga tgctgtgtac caggccgccg agcaatcctt caacgatggt tgagatcatg
     2581 caacttagcg cggtcatgcc ccccatcgaa tatcgatgta agccgccggt cagaccaacc
     2641 agcccaccga cgaccggacc gccgagtaag ccgcccatta ccgcgcctat cgcacgggta
     2701 ttggcaatag aatcgtcaat gtgcaaccca aaccaggtgc ccatgatgca gaagatggaa
     2761 aagacgatgt agcagagaaa tttatgcggc agacgaaccg tgacctgcat taacggtatg
     2821 aataatggcg ttttactcat taaccacgca atgactaaaa aaacgcacat ctgctgaagc
     2881 agcagcaaca ccagattaaa atcgtacata cccgcaaacc acacttccct ttaaaacgcg
     2941 taacatacat tgcctgcgtt taactttctt tgaactcttg cagaaaaatg agaattcgtg
     3001 agtacgatca ctcaaaatcg cctggcaaaa ataaaatcac cctatagatg cacaaaaaac
     3061 gggcaaaact acctggttcg caaaactgcg tctaaagtta aaccgggacc tcgcgagcaa
     3121 gggtgagacg atggcgcttt acacaattgg tgaagtggcg ttgctttgtg atattaaccc
     3181 tgtcacgtta cgcgcgtgac gcaacatttt taaaaatcga cgaagatcgt ccaggagcgc
     3241 cgtttttcaa gggttggata gatgattcgt ttaccatgtc gacgaacttc gacgaatttc
     3301 gccgataaaa agtgtcccat acatgcccca ttgatgcccc ataaacaata tcaggaattg
     3361 acactggtta tccatacaga taaaaataac acctgtacat aaccacagtt acaggagggt
     3421 gttatgcgtg ttgagatctg tatcgctaaa gaaaaaatca ctaaaatgcc aaacggtgct
     3481 gtggatgcgt taaaggaaga attaacccga cgcatcagta aacgttatga cgatgtagag
     3541 gtgatcgtaa aagccaccag caacgatggc ctttctgtta cacgcaccgc agataaggat
     3601 tctgcaaaaa catttgttca ggagactctg aaagatacct gggagtctgc tgacgagtgg
     3661 ttt
//
LOCUS       DAZPAC010000329         3629 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZPAC010000329 DAZPAC010000000
VERSION     DAZPAC010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.333"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..399
                     /gene="tolA"
                     /locus_tag="ACHUPM_004263"
     CDS             <1..399
                     /gene="tolA"
                     /locus_tag="ACHUPM_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR0871875.1"
                     /translation="AAAEADDIFGELSSGKNAPKTGGGAKGNNASSAGSGNTKNNGAS
                     GADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAA
                     LAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            532..1824
                     /gene="tolB"
                     /locus_tag="ACHUPM_004264"
     CDS             532..1824
                     /gene="tolB"
                     /locus_tag="ACHUPM_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR0871876.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            1859..2380
                     /gene="pal"
                     /locus_tag="ACHUPM_004265"
     CDS             1859..2380
                     /gene="pal"
                     /locus_tag="ACHUPM_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR0871877.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            2390..3181
                     /gene="cpoB"
                     /locus_tag="ACHUPM_004266"
     CDS             2390..3181
                     /gene="cpoB"
                     /locus_tag="ACHUPM_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR0871878.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            3346..3421
                     /locus_tag="ACHUPM_004267"
     tRNA            3346..3421
                     /locus_tag="ACHUPM_004267"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3379..3381,aa:Lys,seq:ttt)
BASE COUNT          930 a          877 c          970 g          852 t
ORIGIN      
        1 gctgccgcag aggcagatga tattttcggt gagctaagct ctggtaagaa tgcaccgaaa
       61 acggggggag gggcgaaagg gaacaatgct tcgtctgccg ggagtggtaa tactaaaaac
      121 aatggcgcat caggggccga tatcaataac tatgccgggc agattaaatc tgctatcgaa
      181 agtaagttct atgacgcatc gtcctatgca ggcaaaacct gtacgctgcg cataaaactg
      241 gcacccgatg gtatgttact ggatatcaaa cctgaaggtg gcgatcccgc actttgtcag
      301 gctgcgttgg cagcagctaa acttgcgaag atcccgaaac caccaagcca ggcagtatat
      361 gaagtgttca aaaacgcgcc attggacttc aaaccgtaat cgcgatgttg actgttccga
      421 cggtcaacat caggcaccgg ttgccacggg gttctggtag ttttgtgtat tttagtttgt
      481 taacattctg ctaaattatc gtgggccatc ggtccagata agggagatat gatgaagcag
      541 gcattacgag tagcatttgg ttttctcata ctgtgggcat cagttctgca tgctgaagtc
      601 cgcattgtga tcgacagcgg tgtagattcc ggtcgtccta ttggtgttgt tcctttccag
      661 tgggcgggcc ctggtgcggc acctgaagat attggcggca tcgttgctgc cgacttgcgt
      721 aacagcggta aatttaatcc gttagatcgc gctcgtctgc cacagcagcc gggtagtgcg
      781 caggaagtac aaccagctgc atggtccgcg ctgggtattg acgctgtagt tgtcggtcag
      841 gtcactccga atccggatgg ttcttacaat gttgcttatc aacttgttga cactggcggc
      901 gcaccgggta ctgtacttgc tcagaactcg tacaaagtga acaagcagtg gctgcgctat
      961 gctggtcata ccgccagtga tgaagtgttt gaaaaactga ccggcattaa aggtgcgttc
     1021 cgtacccgta ttgcctacgt tgttcagacc aacggcggtc agttcccgta tgaactgcgc
     1081 gtatctgact atgacggtta caaccagttt gtcgttcacc gttcaccaca gccgctgatg
     1141 tctccggcgt ggtcaccaga cggttctaaa ctggcttatg tgaccttcga aagcggtcgt
     1201 tccgcgctag ttatccaaac gctggcaaac ggcgctgtac gtcaggtggc ttcattcccg
     1261 cgtcacaacg gtgcgcctgc attctcgcca gacggcagca aactggcatt cgccttgtcg
     1321 aaaaccggta gcctgaacct gtacgtaatg gatttggctt ctggtcagat ccgccaggtg
     1381 actgatggtc gcagtaacaa taccgaaccg acctggttcc cggacagcca gaacctggca
     1441 tttacttctg accaggccgg tcgtccacag gtttataaag tgaatatcaa cggcggtgcg
     1501 ccacaacgta ttacctggga aggttcgcag aaccaggatg cggatgtcag cagcgacggt
     1561 aaatttatgg taatggtcag ctccaacggt gggcagcagc acattgccaa acaagatctg
     1621 gcaacgggag gcgtacaagt tctgtcgtcc acgttcctgg atgaaacgcc aagtctggca
     1681 cctaacggca ctatggtaat ctacagctct tctcagggga tgggatccgt gctgaatttg
     1741 gtttctacag atgggcgttt caaagcgcgt cttccggcaa ctgatggaca ggtcaaattc
     1801 cctgcctggt cgccgtatct gtgataataa ttaattgaat agtaaaggaa tcattgaaat
     1861 gcaactgaac aaagtgctga aagggctgat gattgctctg cctgttatgg caattgcggc
     1921 atgttcttcc aacaagaacg ccagcaatga cggcagcgaa ggcatgctgg gtgccggcac
     1981 tggtatggat gcgaacggcg gcaacggcaa catgtcttcc gaagagcagg ctcgtctgca
     2041 aatgcaacag ctgcagcaga acaacatcgt ttacttcgat ctggacaagt acgatatccg
     2101 ttctgacttc gctcaaatgc tggatgcaca tgcaaacttc ctgcgtagca acccgtctta
     2161 caaagtcacc gtagaaggtc acgcggacga acgtggtact ccggaataca acatctccct
     2221 gggtgaacgt cgtgcgaacg ccgttaagat gtacctgcag ggtaaaggcg tttctgcaga
     2281 ccagatctcc atcgtttctt acggtaaaga aaaacctgca gtactgggtc atgacgaagc
     2341 ggcatactcc aaaaaccgtc gtgcggtact ggtttactaa gagaattgca tgagcagtaa
     2401 cttcagacat caactattga gtctgtcgtt actggttggt atagcggccc cctgggccgc
     2461 ttttgctcag gcaccaatca gtagtgtcgg ctcaggctcg gtcgaagacc gcgtcactca
     2521 acttgagcgt atttctaatg ctcacagcca gcttttaacc caactccagc aacaactttc
     2581 tgataatcaa tccgatattg attccctgcg tggtcagatt caggaaaatc agtatcaact
     2641 gaatcaggtc gtggagcggc aaaagcagat cctgttgcag atcgacagcc tcagcagcgg
     2701 tggtgcagcg gcgcaatcaa ccagcagcga tcaaagcggt gcggcggcat caacgacgcc
     2761 gacagctgat gctggtactg cgaatgctgg cgcgccggtg aaaagcggcg atgcaaacac
     2821 ggattacaat gcagctattg cgctggtgca ggataaatcc cgccaggatg acgcaatggt
     2881 ggcatttcag aatttcatca aaaattaccc tgattcaact tacctgccaa acgccaatta
     2941 ttggctgggt cagttaaact acaacaaggg taaaaaagat gatgcggcgt actattttgc
     3001 ttcggtagtg aaaaactatc cgaagtcacc aaaggctgct gatgcgatgt ttaaagtcgg
     3061 cgtcatcatg caggacaaag gtgacaccgc aaaagcgaaa gccgtatacc agcaggttat
     3121 cagtaaatac cctggtactg atggcgctaa acaggcacaa aaacgtctga acgcgatgta
     3181 atgcataaca cacgaccaga agtcgcatta tttctggtcg tgtcgtgcga atcataagca
     3241 gttgagtgat ctgcatcgaa atttttgttg cgctcaagtc tgaaatcagt aatatatgcc
     3301 gccgttgcca cgggatatca aacaaaccga aagcaacgaa aaagtgggtc gttagctcag
     3361 ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc
     3421 agttttagca tcgaagacag atgttaagcg tgtaggataa cgttgcgtca gcaacggccc
     3481 gtagggcgag cgaagcgagt catcctgcac gacccaccac taattacggt gggttcggtg
     3541 gaagtagttt gtagtatcca gcgcagtatc gggtgattag ctcagctggg agagcacctc
     3601 ccttacaagg agggggtcgg cggttcgat
//
LOCUS       DAZPAC010000330         3577 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZPAC010000330 DAZPAC010000000
VERSION     DAZPAC010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.334"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHUPM_004268"
     CDS             <1..196
                     /locus_tag="ACHUPM_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871879.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            207..374
                     /locus_tag="ACHUPM_004269"
     CDS             207..374
                     /locus_tag="ACHUPM_004269"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871880.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            371..715
                     /locus_tag="ACHUPM_004270"
     CDS             371..715
                     /locus_tag="ACHUPM_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871881.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            1007..1162
                     /gene="hokD"
                     /locus_tag="ACHUPM_004271"
     CDS             1007..1162
                     /gene="hokD"
                     /locus_tag="ACHUPM_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0871882.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1383..1469
                     /locus_tag="ACHUPM_004272"
                     /pseudo
     CDS             1383..1469
                     /locus_tag="ACHUPM_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1466..1738
                     /locus_tag="ACHUPM_004273"
     CDS             1466..1738
                     /locus_tag="ACHUPM_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871883.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1740..2798
                     /locus_tag="ACHUPM_004274"
     CDS             1740..2798
                     /locus_tag="ACHUPM_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR0871884.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIALLPHVHQQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            2799..3179
                     /locus_tag="ACHUPM_004275"
     CDS             2799..3179
                     /locus_tag="ACHUPM_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0871885.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3176..>3577
                     /locus_tag="ACHUPM_004276"
     CDS             3176..>3577
                     /locus_tag="ACHUPM_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0871886.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT          968 a          808 c          980 g          821 t
ORIGIN      
        1 atcaccgaaa aaaattggct tttacaccat tggcgataaa agcatttacc tgtatgacct
       61 acgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcgatgg attggtgtgt
      121 tgctgttcat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
      181 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
      241 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
      301 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
      361 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
      421 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
      481 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
      541 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      601 aaagcagtta acctggcaac tgaggaaagc gaagccagac tgcaaattac cggataaggc
      661 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      721 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      781 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      841 gcttgaggct atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      901 aacattacgc gtccggcaag acgcttaaca ttaatctgag gctcaatcta tgaacggcaa
      961 atctaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     1021 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     1081 gaggaaagac ctctgcgagg tacgaattcg aaccggccag acggaggtcg ctgtcttcac
     1141 agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc cacctctgat
     1201 gggtcaggca tcctcaatgc acccacactt aacccgcttc ggcgggtttt tgtttttatt
     1261 ttcaacgcgt ttgaagtttt agatggtgcc ggaatagaat caaaaatact taagtagcgc
     1321 gcagggagaa gagggatgga ccccgaacag gggagtgcta tttatctgga aggattctgt
     1381 tgatgaaaat cgaagaatta cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg
     1441 ttgagaatgg cgctattgtc agccactgcc ttccagacta cacaattctg ataattcagc
     1501 cgtctttgcc agcaggcacg ggcggcgctc tcacgcattt aaatctgatt ggtaccagca
     1561 tcccccatgc actgaagaac aggccgaatg gctcattcag tgttaccgca ggcgcggatg
     1621 cgaggttaaa aaagccctta gcctcgacta ccgtcactgg ataatctccg tcaggctccc
     1681 ttactccgaa cggccaacgc gtccgtcccg cacattccag caacggatct ggaggtaatg
     1741 tgcgggtatt acttcgacct gttctggtac cggaactcgg tctggttatc gttaagccag
     1801 gccgtgaatc aatgtcagca ttccataacg gcagaatact ggtggagccg gaaccaaaaa
     1861 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgctcg ccagccgctg gtggaagaca
     1921 aaacattgct gccgtttttc agtaacgcac gggtgattcg tgctgctggt ggtgctggtg
     1981 cattgtctga ctggctgttg cgccatatta aatcctgcca gtggccacac ggcgattatc
     2041 atcacagcga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     2101 gcgacaacca gctgcgtgac cagacatccg aatcactcga gcaacttgct catcaaaacc
     2161 tgtcagcatg gatgattgac gtcatcggtc acgcaataag cggtacgcag gagcgtgaat
     2221 tatctctggc tgaattatcc tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg
     2281 aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa aattcgctct gtgtacagtg
     2341 aaagcgacat cataccggga gagcagacag ccaccagcat actgaagcag cgcacaaaaa
     2401 atattgcgtt actgcctcac gtccaccagc aacagaaccc accacaggaa aagacggtgg
     2461 tcagcattgc cgttgatccg gagtcaccgg ctcagtatct ccagcgccag aaatcacaac
     2521 gggaagagat gcctgtatac acgcgctggg taaaaacgca gaaatgcatg acgtgtggca
     2581 atcaggcaga tgatccgcat cacatcattg gtcatggact gggagggatg ggaacaaagg
     2641 ctgatgattt gtttgttatt ccgttatgcc gtaaatgccg taacgaacta catgccggag
     2701 taaaagattt tgaagagaaa cacggcagtc agctgttgtt gctgattcgt tttttaatgc
     2761 acgcgagaaa ttcgggtgtt ctgaagtgga aagcataaat gaccgagcgc atagaatttg
     2821 ttttacctta cccgccgacg gtgaacactt actggcgtcg tcgtggcagc acatattttg
     2881 tatcaaaagc cggggagcgt tatcgccggg cagtggcgct tattgttcgc cagcagcggc
     2941 tgaaattaag cctgtccgga aggttggcaa taaaaattat tgcagaacca ccggataagc
     3001 gccgccgtga cctggacaat attctgaaag cgccgctgga tgcgctgacg catgcggggt
     3061 tgctaatgga cgatgagcag tttgatgaaa tcaatatcgt tcgtgctcag ccagtatctg
     3121 gtggacgtct gggggtgaag atttatccca taatgcttga agggcaggtc aaaaaatgaa
     3181 actggaagat ttaccgaaat actactcccc aaaatccccc ggcctgactg atgcatcggc
     3241 ctcaacgtcg aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg gcatgacaca
     3301 gaatcgggct gagatggggg tttctgcgtt ccttgggaaa atgggcatta gtatgaatga
     3361 cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg atcgcgtggc
     3421 ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta tggcttcgta
     3481 tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgcccct gctgtcacgg
     3541 aaaaaaattt attgaaagcg aggtttttac aaacaag
//
LOCUS       DAZPAC010000331         3559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZPAC010000331 DAZPAC010000000
VERSION     DAZPAC010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.336"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            129..1376
                     /locus_tag="ACHUPM_004277"
     CDS             129..1376
                     /locus_tag="ACHUPM_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFR0871887.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHUPM_004278"
     CDS             complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHUPM_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFR0871888.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2228..2572)
                     /locus_tag="ACHUPM_004279"
     CDS             complement(2228..2572)
                     /locus_tag="ACHUPM_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871889.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2814..3416
                     /locus_tag="ACHUPM_004280"
     CDS             2814..3416
                     /locus_tag="ACHUPM_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871890.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          886 a          714 c          817 g         1142 t
ORIGIN      
        1 gcagtaacgg cgaaaggggc aatgacagcg ctgccatgtc gacaagaact ggaatagtga
       61 gaagtaaacg gcgaagtcgc tcttatctct aaataggacg tgaatttttt aacgacaggt
      121 aggtaattat ggcactgaat attccattca gaaatgcgta ctatcgtttt gcatccagtt
      181 actcatttct cttttttatt tcctggtcgc tgtggtggtc gttatacgct atttggctga
      241 aaggacatct agggttgaca gggacggaat taggtacact ttattcggtc aaccagttta
      301 ccagcattct atttatgatg ttctacggca tcgttcagga taaactcggt ctgaagaaac
      361 cgctcatctg gtgtatgagt ttcatcctgg tcttgaccgg accgtttatg atttacgttt
      421 atgaaccgtt actgcaaagc aatttttctg taggtctaat tctgggagcg ctcttttttg
      481 gcctggggta tctggcggga tgcggtttgc ttgacagctt caccgaaaaa atggcgcgaa
      541 attttcattt cgaatatgga acagcgcgcg cctggggatc ttttggctat gctattggcg
      601 cgttctttgc cggcatattt tttagtatca gtccccatat caacttctgg ttggtctcgc
      661 tatttggcgc tgtatttatg atgatcaaca tgtgttttaa agataaggat caccagtgcg
      721 tagcggcgga tgcgggaggg gtaaaaaaag aggattttat cgcagttttc aaggatcgaa
      781 acttctgggt tttcgtcata tttattgtgg ggacgtggtc tttctataac atttttgatc
      841 aacaactttt tcctgtcttt tatgcaggtt tattcgaatc acacgatgta ggaacgcgcc
      901 tgtatggtta tctcaactca ttccaggtgg tactcgaagc gctatgcatg gcgattattc
      961 ctttctttgt gaatcgggta gggccaaaaa atgcattact tatcggtgtt gtgattatgg
     1021 cgttgcgtat cctttcctgc gcgctgttcg ttaacccctg gattatttca ttagtgaagc
     1081 tgttacatgc cattgaggtt ccactttgtg tcatatccgt cttcaaatac agcgtggcaa
     1141 attttgataa gcgcctgtcg tcgacgatct ttctgattgg ttttcaaatt gccagttcgc
     1201 ttgggattgt gctgctttca acgccgactg ggatactctt tgaccacgca ggctaccaga
     1261 cagttttctt cgcaatttcg ggtattgtct gcctaatgtt gctatttggc attttcttcc
     1321 tgagtaaaaa acgcgagcaa atagttatgg aaacgcctgt accttcagca atatagacgt
     1381 aaacttttcc ggttgttgtc gatatctccc tatccctcaa ccggaaaata ataatactaa
     1441 agtgcttagc ccggctaata atcacctaat ccaaacgcct cattcatgtt ctggtacagt
     1501 cgctcaaatg tacttcagat gcgcggttcg ctgatttcca ggacattgtc gtcattcagc
     1561 gacctgtccc gtgtatcacg gtcctgcgaa ttcatcaagg aatgcattgc ggagtgaagt
     1621 atcgagtcac gccatatttc gctatcagga ttctgtgtga tggttacatc gcccggccca
     1681 gggctgttta gtcatcagcg ctttctgaca gtgctgagat ttcaacctgt tgcagtaaaa
     1741 atgagtagat ataaggcaag tgtgctgcca aacccatctt ttacggggtg aaggtagatt
     1801 tcgtttgaag ggtatctggt gtcccctgca gacatctact tgaggcggca ggggattgat
     1861 tggaatgctg ttttttagat ttgaaaaata ttttacccgc tattttaccc attggcgcgg
     1921 cttaagagct tatttttgaa ttcacaatgg tcacgatata accatcttgc tcgtccgtgg
     1981 ataactttgg ctttaggcag gtcgccggac ttaatccggt catagatgaa ggtcttaccg
     2041 aagccagtat cagccatgat gaatttcaaa tcaaccagtg aatcaggttg tagttcgtgt
     2101 tgcatgagtg ctatctccga atatggaatc gaacctgcaa atcaggcaat aaaaagccgc
     2161 attgatgcag caatggtagg tctggatatc ttgagaaatg aacaggcctc atcgagtgtg
     2221 aggcgggtta gtccttgcgt agctcgctga ttcttctgta agtctctggt gctttgttcc
     2281 cgtacgtctt catttcagac ttcaacagag caacgagtga atcccattcg ttgaggattc
     2341 ctttgaatgc cggaacgcgc tttgcaacct tgtcgaatga atctctgatt tctggaatct
     2401 gctcaacaag tgcaacgcat cgccgaaagt ctgctgcgtc atggagagcg ccgaagtgat
     2461 gaccatagat attctttttc aggccacatg cgattgaggc aagagttgcg ctactgatgc
     2521 cgacatcgcc agttgattgc catttcaaaa ccttcatagc caaatctgac atttcttgtc
     2581 tccataaaac aaaactcgcc gtagcgagct cagataaaag aaatccccgt cagtgcgagg
     2641 atgctgttca ttgctgctat acactttttt gctctcaacg taagcggtag cccattctgt
     2701 tgggttggtc agttgctttt aggaaatgct ctttacccct taaacgtcgg ctgaaagagc
     2761 taaaatccat gcaaaaaatt tacgcaattt tgtgtattat tgtgcagtaa gtaatgagct
     2821 attttctgtg caaaaaatgg atgataaatt tgtccggggc aggaaaaatt ttatgggcgc
     2881 taaacatgaa aaaagattcg tatccttatt tgatttgcat gacagtttca gggctgatct
     2941 ttattttcct tttcttctgg tggcgggcag atatctacag ggtcacgttt cttaatcaga
     3001 gtatatccca ctattacatt ctgtttagca tgggaatagc ttttctgtta tctctgtttt
     3061 gggttaagaa ggggatagta aaacaaagcg gctggaagag tctgtcagca taccttaagg
     3121 tttatgcagg gatatgcata ttttctggat tttttctgat tatacccctt acaacactaa
     3181 cttatttttt gcctggagag acatcgtctt atgttgcacc gtatcggtat acttccggta
     3241 gttcaaaaag ttgttctgga gctgaggtgg atgaccccga tctacatgag aatattcgca
     3301 tttgctatcc gtatggcaat tatgagtacg ataatattat ctatgttgaa aagaaaatta
     3361 atacattagg tgcggtagtg acatatgcac agaccgcgcg tgatgatact gaatgagata
     3421 gtatatagcg ggcaagtttt agttaattta tcgaggtaat ataatttacc tcgactcgtt
     3481 tattctggta tgaatatttc gctttacgat cgatttttat ctgatgatat catgcggttt
     3541 tcatatactg acttactgt
//
LOCUS       DAZPAC010000332         3551 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZPAC010000332 DAZPAC010000000
VERSION     DAZPAC010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.337"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(41..1900)
                     /gene="gss"
                     /locus_tag="ACHUPM_004281"
     CDS             complement(41..1900)
                     /gene="gss"
                     /locus_tag="ACHUPM_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR0871891.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2105..2971
                     /gene="yghU"
                     /locus_tag="ACHUPM_004282"
     CDS             2105..2971
                     /gene="yghU"
                     /locus_tag="ACHUPM_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR0871892.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3000..3081)
                     /locus_tag="ACHUPM_004283"
                     /pseudo
     CDS             complement(3000..3081)
                     /locus_tag="ACHUPM_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3094..3342)
                     /gene="hybG"
                     /locus_tag="ACHUPM_004284"
     CDS             complement(3094..3342)
                     /gene="hybG"
                     /locus_tag="ACHUPM_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR0871893.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3355..>3551)
                     /locus_tag="ACHUPM_004285"
     CDS             complement(3355..>3551)
                     /locus_tag="ACHUPM_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase/urease nickel incorporation protein"
                     /protein_id="HFR0871894.1"
                     /translation="IVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCH
                     GERLRVDTGDSLIVKSIEVE"
BASE COUNT          837 a          971 c          886 g          857 t
ORIGIN      
        1 attattcttc ataatcagta cggggttgct acaacatgta ttactttttc accacaatta
       61 acggttcaat atcactctct tttttgatga ccagtgattc atcaccgcgc aaacacgtcc
      121 caccgtagtt gccgcccacg gtgaaggtac atacctgaat gtatttaccg tccactttcg
      181 gcaaacacca cagttgctga tagatgtttt tctgctcggc aaatttaccg ctggttttgt
      241 ccagcacctc ttcatgatgg ctgaccaggt cgatattgct accacagcga ccggcaatcg
      301 gtttcactgc gtaacctgtt ttcaccaatt catcattaac agtgaaatcg gtatccagca
      361 ggtaacgatg gtgcgggaac agcgaccaga ggatcggcag aatcgctttg ttgccgggga
      421 tcaccgtcca cagcggctca aagactagca cttccgggcg cagcaatacg tcgataagac
      481 gcacttcgtt ttgcggatga ccggtacgga ttggcaccgc agcaaactca cggtcgctaa
      541 cttcacgaat ctgatcaaac gcggtttccc acgcccaggt tttccacacg cagttaacca
      601 gtcgcccttc cccatcaatc agttgcccgg cagcatccca gcccagttca tccaatccac
      661 gcaagatacg cgtttcaaag cccgcctggt gcagcgcctg ctccataaac tgcgcgtgat
      721 agttttcctc gatatctttg tcctgcatga tatggacaaa cggacgtgca cgactgtgtt
      781 tccaggcacc agccaattcg ttaatcagcc cttccgccgg attgaagccg ttgcctttat
      841 agccctgctc cgcccaacgt tcgaggatca agcccgcttc ggtatgacag gaggcggagt
      901 cggcgttgta ctcgtaaacc ttcaggccac gctcatccat gcagaaatcc atacgaccag
      961 tgatcatatg gtgacggcga cgctgccagg agagacgcaa acgtggccag aggattttcg
     1021 ggatgtcgaa cagcgccagc aggttgtcat ctttcagcac cttgtcggtt gcgtgaagat
     1081 acatcaggtg cagctcgttg gtggctttaa ttagctcctg ctcggcactc tcggtaatgg
     1141 tgtagtaatg ataaggatcc tgattgatca cctgaccgtt ggcctgcaca taggcgtttt
     1201 gcagcggatc tttttcatcc agccatttac cgtcaaactg gcctttgttt tccaggcgcg
     1261 ctccgctgat tttcagcagc tcgcctgcaa tttccggctg cggtaagctg tattcagtat
     1321 cttccgtctg gatcatccag cccagaatgg tggtgtcatc aaaagtgtct ttcagggtat
     1381 agcagccgtt ttcgaccacc atctccagct cacgcgtcca ctgttgccct tgcggcaacg
     1441 gggaatgaat cacgttctgt tccgcaatac ggactttgtt gccatgcaat tgggtaatga
     1501 tggcgacatg gccagtgtct ttaaattcac cgcctttatc ccagataaga agcgcacccg
     1561 cgaccggcgc acgcggcgag ccgttaggaa atgcctgcaa tggcaggatg ttgtcattaa
     1621 ccacttctcg caggaagcgc agcgagaaaa tctcccacgc catacccacg tcagtaaaga
     1681 ccacaccgta attcagaaag agaaaacggc gagcaaattc aacgcattgc cacttgtggc
     1741 ccatatattc gtcgtcgata tagctacgga atacggcgtc atcttcgtat tcctgcggat
     1801 cgagagaact gtaatctgaa gagtagattg ctaccccacc tggggcgtag cccaataatg
     1861 tcccgaacgg ggcatcctgg ctggtcgttc ctttgctcat cactttacct ttaacaatac
     1921 aacctaagca gttggcgtaa ttgttgtgct ctgattacct gctcagcgac attaaccgga
     1981 cagaggtcag actaatcata cacctcatcg cagcggctgg cgcaaagggt taaaaaattc
     2041 acattctgta cagcaagtga cctgctacac tgcttcaaca ctaccactca gaaggcaact
     2101 cactatgaca gacaatactt atcagcccgc gaaagtctgg acgtgggata aatccgctgg
     2161 cggcgcgttc gccaatatca atcgcccggt ttctggtccg acgcatgaaa aaacgctgcc
     2221 cgttggcaaa cacccattgc aactttattc gctgggaacg ccgaacggtc agaaagtaac
     2281 gattatgctt gaggagctgc tggcgctggg cgttactggt gcagagtacg acgcctggct
     2341 gattcgtatt ggcgatggcg atcaattctc cagcggcttt gtcgaagtga acccaaactc
     2401 gaagatcccg gcgctgcgcg atcatacgca taatccgccg atccgcgtgt ttgaatctgg
     2461 ttcgatcctg ctttatctgg cggagaaatt tggctacttc ctgccgcagg atttggcaaa
     2521 gcgtactgaa acgatgaact ggctgttctg gttacagggc gcggcaccgt tcctcggcgg
     2581 tggttttggt cacttttacc attacgcgcc gatgaaaatt gagtacgcca tcaaccgctt
     2641 taccatggaa gccaaacgtc tgctcgacgt gctggataag caactggcgc agcataagtt
     2701 tgttgcgggc gatgagtaca ccattgcgga tatggcgatt tggccgtggt ttggcaatgt
     2761 ggtgttaggc ggcgtgtatg atgccgcaga gtttcttgat gcgggcagtt ataagcatgt
     2821 acaacgctgg gcgaaagaag taggcgaacg tccggcggtg aaacgtgggc gtattgttaa
     2881 ccgcaccaac ggaccgctga atgagcagtt gcatgagcgc catgacgcca gtgatttcga
     2941 gacgaatacg gaagataagc gtcaggggta agggttggtg ttcgtcgcag caagccatcc
     3001 aggccggata aggcgttcac gccgcatccg gcaatcgtgc acaatgcctg atgcgacgct
     3061 gccgcgtctt atcaggccta cgaaagctaa tcatcacgcg ctggtaatgt cgtaatccat
     3121 ttggcgcagt gcgtctaatg tggctttggc ttcatcttcg tcgatgatgc tcatggcaaa
     3181 tccgacgtgt accagcaccc actggcccag tagatcggca gggttacctt cacaaatcag
     3241 ggcgatattc acgtcgcgct tgataccaca tacttcaacc tgcgcaagct ggtgaatatc
     3301 ttcaccgaca gccagcacct ggcctggaac gccaatacac attattaact ccggttattc
     3361 aacttcaata cttttgacga tcagcgaatc gccggtatcg acacgcaacc gctcgccgtg
     3421 acagagcgga cactgcgcat cgtgctgatg aatctccacc acctggctgc aatcccagca
     3481 ccaagcctgg gcgggtttat agacgatatg taaatcgcac ccttgcgcca ccgttccgtg
     3541 gcagacaatt t
//
LOCUS       DAZPAC010000333         3550 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZPAC010000333 DAZPAC010000000
VERSION     DAZPAC010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.338"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            324..1430
                     /locus_tag="ACHUPM_004286"
     CDS             324..1430
                     /locus_tag="ACHUPM_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFR0871895.1"
                     /translation="MSKASEPQQTPYLIPQGVYPTYMPKAEDEIDLFELLGTLWKKKW
                     VILCVTLLTTGLAAVYAFTAKEQWTAKTYIQAPRIAELGSYLKFHQAYARILNQPLDT
                     NALANGLFSDLILIAESPDTKVKFLESTEYYKKETNNLSTEQDKKIWLAEQANKGLVI
                     TPPKEKGNTSYYIIQASADSAQEAYKLLQGYLKNVNNQAVTLSLDEFGQNVNTLLVNL
                     NKEIIDIDFQRKSEKLDQIAHIQRDLTTAEQAGIIDYRSSKGGFDNAQSSYKFLLGEK
                     LLSAELKATKDAPIIYPFRYYEVKRQIDELEGMLRDNIQAQAYRYQMKPSEPVIKDKP
                     NKALILILGALPGAMFAIVGTLVYATLKDKTKLD"
     gene            1499..2779
                     /gene="tviB"
                     /locus_tag="ACHUPM_004287"
     CDS             1499..2779
                     /gene="tviB"
                     /locus_tag="ACHUPM_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HFR0871896.1"
                     /translation="MKFDTLNAKIGIIGLGYVGLPLAVEFGKKVTTIGFDINKSRIDE
                     LRNGHDSTLECSNLELLEATKLTYACSLDALKECNVFIVTVPTPIDKHKQPDLTPLIK
                     ASETLGKIIKKGDVIIYESTVYPGATEEDCIPVVEKVSGLKFNIDFFAGYSPERINPG
                     DKEHRVTNILKVTSGSTPDVAEYVDQLYKLIITVGTHKASSIKVAEAAKVIENTQRDV
                     NIALINELSIIFNKLGIDTLEVLEAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQS
                     VGYHPEMILAGRRLNDSMGQYVVSQLVKKMLKQRIQVEGANVLVMGLTFKENCPDLRN
                     TKVIDIISELKEYNINIDIIDPWCSTDEAQHEYGLTLCEDPKVNHYDAIIIAVAHNEF
                     REMGESAIRALGKDEHVLFDLKYVLDKKSIDMRL"
     gene            2872..>3550
                     /locus_tag="ACHUPM_004288"
     CDS             2872..>3550
                     /locus_tag="ACHUPM_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR0871897.1"
                     /translation="MSRYEEITQQLIFSPKTWLITGVAGFIGSNLLEKLLKLNQVVIG
                     LDNFSTGHQYNLDEVKTLVSTEQWSRFCFIEGDIRDLTTCEQVMKGVDHVLHQAALGS
                     VPRSIVDPITTNATNITGFLNILHAAKNAQVQSFTYAASSSTYGDHPALPKVEENIGN
                     PLSPYAVTKYVNEIYAQVYARTYGFKTIGLRYFNVFGRRQDPNGAYAAVIPKWTAAML
                     KGDDVYIN"
BASE COUNT         1150 a          574 c          731 g         1095 t
ORIGIN      
        1 tcacagaatt tacaaacata ctgttatcat tttgaaggca gatttggtct tatacaggca
       61 ttgctttata atctgcactc caaattctgc gggctatccg ccggtttgca gcagggaagt
      121 gtgggactgt atatgtctct tcacacggag tgttctcgat tatgtcctct aatcccagat
      181 atcacttgtt gtatcgcagt tggctatatc ctgtttctgc gcagcgcttt gggagctgaa
      241 actcaagggc ggtagcgtac ttttttgtca ggcttattct tcatttttat ttttaaccca
      301 ttgataaata atggattggt ttcatgtcaa aagcatctga accacaacag accccttatc
      361 tgatcccgca aggggtctat ccaacttata tgccaaaagc agaggatgaa atcgatcttt
      421 tcgagctttt aggcaccttg tggaagaaaa aatgggttat tttatgtgtc acgttgctga
      481 ctacggggtt agcagcagtg tatgccttta ccgcaaaaga gcagtggacg gctaaaacct
      541 atattcaagc accacgtatt gctgaattag gcagctatct taaatttcac caagcgtatg
      601 cccgaatatt aaatcaaccg ttagatacga atgcgttggc taatggattg ttttccgatt
      661 tgattttgat tgctgaatcg ccagacacca aagttaaatt tctagagagt actgagtatt
      721 ataaaaagga aacaaataat ttatctactg aacaagataa gaaaatttgg ttagctgagc
      781 aagcgaataa aggtcttgtg attacgccac caaaggaaaa gggaaataca agttactaca
      841 taatacaagc atcggcagac tcagcgcaag aggcatataa actactgcag ggatatctaa
      901 agaatgttaa taatcaagct gtaacattaa gtcttgatga gtttggtcaa aatgttaata
      961 ctcttttggt taatctaaat aaagaaatta ttgacataga tttccagaga aaatcagaaa
     1021 agcttgatca aatagctcat attcagcgag atttaacaac tgcggaacaa gccggaatca
     1081 ttgattatcg ctctagcaaa ggcggcttcg ataatgcgca aagtagctat aagttcttgc
     1141 tcggcgaaaa actgttatca gcagagctaa aagcaactaa agatgcgcca attatttacc
     1201 catttagata ttacgaagtg aaacgtcaaa ttgatgagtt agaaggaatg ttacgcgata
     1261 acattcaggc gcaagcatat cgatatcaaa tgaagccatc tgagccagtt ataaaagaca
     1321 aacccaacaa agcattaatt ttgattcttg gtgcattacc aggggcaatg tttgctatag
     1381 ttggtacatt agtttatgcg acattaaaag ataaaaccaa gttagattaa actgggttac
     1441 gtattgttgt gtcaatgcga aatagatgtt ctatgtgcac tttatgatgg ataagaaaat
     1501 gaaattcgat actttgaatg cgaaaattgg gattataggc cttggttatg ttggattgcc
     1561 tcttgctgtt gagtttggaa agaaagtaac gacgattgga tttgatatta ataagtctcg
     1621 tattgatgaa ttacgaaatg gtcacgatag tacattagag tgctcaaatt tagagttgtt
     1681 agaagcaact aaattgacgt acgcctgttc attagatgca ctaaaagagt gtaatgtatt
     1741 tattgtaact gttccaactc caattgataa acataaacag ccagatctaa cacctctaat
     1801 taaagcatct gaaacattgg gtaagataat aaagaaaggc gatgttatta tttatgagtc
     1861 aacagtttac cctggagcga cagaagaaga ttgtatacca gttgtagaga aagtatcagg
     1921 tcttaagttt aatattgatt tttttgccgg ttattcacct gagcgtatta atcctgggga
     1981 taaagagcat cgtgtaacta atatccttaa ggtgaccagt ggatctacac cggatgttgc
     2041 tgagtatgta gatcagctat ataaattaat aattactgtc ggtacgcata aagcatcatc
     2101 gataaaagta gcagaggctg caaaagtaat tgaaaacacg cagcgagatg tcaatattgc
     2161 attgattaat gagttatcta ttatatttaa taagttaggg attgatacct tagaggttct
     2221 tgaggctgca ggtacgaagt ggaatttttt accttttagg cccggtttag taggtggcca
     2281 ctgtataggt gtagatcctt attatcttac acataaagcg caaagtgtcg gctatcatcc
     2341 ggagatgatt ttagccggac gtcgtttaaa tgatagtatg gggcagtatg tcgtttccca
     2401 gttagtcaaa aaaatgttga aacaacggat tcaagttgaa ggggcgaatg tgttagtgat
     2461 ggggcttaca tttaaagaga attgcccaga tctacgaaac actaaagtga ttgatattat
     2521 ttcagagtta aaagaataca atatcaatat agatattata gatccatggt gttctaccga
     2581 tgaggcacaa catgaatatg gattaacttt atgtgaagat cctaaagtta atcattatga
     2641 tgcaataatt atcgctgttg cacacaatga gtttcgcgag atgggagaga gcgctattcg
     2701 tgcattaggt aaagacgagc acgttttgtt cgatttaaaa tatgtgcttg ataaaaaaag
     2761 tatcgatatg cgcttgtaag agtgattaaa aaaatcaaat cctctttgat atgatacacc
     2821 tcagcatttt atgctaggtt tagcacttga ttaatataca tggatattta tatgtctcgc
     2881 tatgaagaga ttacacagca gttaattttt tcaccgaaaa cttggttaat tactggtgtc
     2941 gctggcttta taggatcaaa tcttttagaa aagttactta aattaaacca ggttgttatt
     3001 gggttagata acttttccac gggacatcaa tataatcttg atgaagttaa aacattagtt
     3061 tccactgaac agtggagtcg attttgcttt atagaaggtg atattcgaga tctcactacc
     3121 tgtgagcaag ttatgaaagg tgttgatcat gtcttacatc aggctgcgct aggttctgta
     3181 cctcgttcaa ttgttgatcc tataacaacc aatgcaacta atattactgg atttttgaat
     3241 atcttacatg cggctaaaaa tgcacaagta caaagtttta cttatgctgc atcaagctca
     3301 acttatggag atcatcccgc actaccaaaa gtagaggaaa acattggtaa tccactttct
     3361 ccttatgcag ttactaaata tgttaacgag atttatgctc aggtatatgc tcgaacatat
     3421 ggttttaaaa ctattggatt acgttatttt aatgtatttg gtcgtcgtca agatcctaat
     3481 ggagcttatg ctgcagtaat tccaaaatgg acagcagcaa tgcttaaagg tgatgacgta
     3541 tatattaatg
//
LOCUS       DAZPAC010000334         3544 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZPAC010000334 DAZPAC010000000
VERSION     DAZPAC010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.339"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /locus_tag="ACHUPM_004289"
     CDS             complement(<1..140)
                     /locus_tag="ACHUPM_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR0871898.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHUPM_004290"
     CDS             complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHUPM_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR0871899.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(696..2151)
                     /gene="xylB"
                     /locus_tag="ACHUPM_004291"
                     /pseudo
     CDS             complement(696..2151)
                     /gene="xylB"
                     /locus_tag="ACHUPM_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
     gene            complement(2223..>3544)
                     /gene="xylA"
                     /locus_tag="ACHUPM_004292"
     CDS             complement(2223..>3544)
                     /gene="xylA"
                     /locus_tag="ACHUPM_004292"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFR0871900.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          924 a          983 c          783 g          854 t
ORIGIN      
        1 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacaga
       61 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
      121 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
      181 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
      241 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
      301 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
      361 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga
      421 aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag ataaaccagc
      481 gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac gatctccata
      541 tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaagaat gccattaaca
      601 aatgattttc agaataaatt catactaaat attaattaat tagtgagata tatagatgtg
      661 aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc
      721 tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg ctgcgcatct
      781 ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga tttctctgga
      841 ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc ccccccccgt
      901 acggtaatcg agcttctgac cgctgatatc cgccagcatc tgacgccagt actcactacg
      961 cgccccgcca ccaatcaacg taacactttg cggtttaatg ccgcaggcat gcacgacatc
     1021 catgccatct gccagcgcat aacccacgcc ttccagcact gctcgcgcca gttcattggg
     1081 gccatgttga tgagtcaaac caaagaaaac ccccttcgcc tggggattat tgtgtggtgt
     1141 acgctcgccg gaaagataag gcagaaacca aactggctcg acactttcat cagcctgttg
     1201 agctgcagcg attaaagctg ggacattgca caggccggtt aatttcgcgg cccaatccag
     1261 acacgatgct gcactcagca ttacagacat taaatgccaa cgctgcggta gcgcatggca
     1321 aaagctgtgt acagcgcttt ctggcttgct taagaaccct tcgctgacag caaaatagac
     1381 ccccgacgtc cccagcgata acattgcctg attagcatca accattccca caccaactgc
     1441 accagctgca ttgtcgccac cgcctgcgac aactggcacc gtcgccatac cccacgcttt
     1501 cgcaacttca ggtaacaaag caccagtaat ttcgctgcct tcgtataatg cgggcatctg
     1561 gtcacgagat aagtcgcaag cctgcagcat gacgtcactc cagtcacgct ttgcgacatc
     1621 cagccacatg gtgccagctg cgtcagacat atcgctggca aactcccccg tcatacgcag
     1681 acgcaagtaa tctttcggta ataatacttt gtcgatttga cggaatatct ccggctcatg
     1741 ccgctgaacc catagcaatt taggcgcagt aaatccgggc atcatcaggt tgccggtaat
     1801 cactcgtgat tgcggaactc tcgcttccag caaagtgcac tcttgcgcac agcgcccgtc
     1861 gttccacaaa atggcagggc gcaatacccg ttgttgagca tccagtaagg ttgctccatg
     1921 catctggccg gcaataccca atgctttaac gtcctgcaga gaatgctgat cgcccagagc
     1981 tttcattgcg cgatcagttg cctgccacca ctgttccggg tcttgttccg accagagtgg
     2041 atgcgggcgc gaaacggtca gcttttccgt ttgcgaatca accacctcac cctgctcgtt
     2101 gagcaaaata acttttacgc ccgaggtgcc aagatctatc ccgatataca tatcgatcgt
     2161 tccttaaaaa aatgcccggt atcgctaccg ataaccgggc caacggactg cacagttagc
     2221 cgttatttgt cgaacagata atgatttacc agattttcca gttgctcctg gcgaccactc
     2281 tgatgcaccg gagacaaatt atgttcctga gcatatttgg ctaaatctgc cagtgacatt
     2341 tggcctttca ggatttgctg gcccaattcg ctattccagc cggaataacg ctgcgcgatg
     2401 cgtttatcca gctcgccatc ttcaatcatg cgcgctgcaa ttttcagcgc cagtgccatc
     2461 gtatccatcg cgccgatatg accgtaaaac agatcatatt tatcagtact ttgacgacgt
     2521 actttggcat cgaagttcag accaccggtg gtgaaaccgc ctgctttgag aatttcatac
     2581 atcaccagcg cattctcttc cacactgttc gggaactggt cggtgtccca gcccagttgc
     2641 gcatcgccac ggttggcgtc gacagaaccg aacaggccaa gcgcaatggc ggtggctatt
     2701 tcatgatgga aagagtgacc tgccagcgtc gcgtggttag cttcaatgtt cagtttaatc
     2761 tctttttcca gaccaaactg tttcaggaag ccatagaccg tcgcggcatc gtaatcatat
     2821 tgatgtttag tcggttcttg cggtttcggt tcgataagca acgtgccctg gaagccgatt
     2881 ttatgtttat gctcaaccac catctgcata aagcggccca gttgttcacg ctcctgacgc
     2941 aagtcggtat ttaacagcgt ttcgtaacct tcacgaccac cccacaggac atagttttca
     3001 ccgcccaatt tatgggttgc ttccatcgct gtaacaactt gcgttgccgc ccagctgaag
     3061 acttcaggat ctgggttcgt cgccgcaccc gcgccatagc gagggtttgt aaagcagtta
     3121 gcggttcccc acagcagctt cacgccgctc tcttcttgct tggctgccag gacatcaacc
     3181 atttgcgcaa aattattgat gtactctttt aacgacgcgc cctcagggga aacatccaca
     3241 tcgtggaagc aataaaatgg cacatgtaac ttgtggaaaa actcaaatgc gacatctgct
     3301 ttacgcttcg ccaacgccag tgcctcacca ggctgctgcc acggacgatt aaacgccccc
     3361 acaccaaaca tgtccgcccc gttccagcag aaggtgtgcc agtagcaggc ggcaaaacgc
     3421 aagtgctctt ccatacgctt acccaacacc agttcgtcgg gattgtagtg acggaatgct
     3481 aacgggtttg aggattttga gccttcataa cgaacgcgat cgagctggtc aaaataggct
     3541 tgca
//
LOCUS       DAZPAC010000335         3530 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZPAC010000335 DAZPAC010000000
VERSION     DAZPAC010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.340"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            9..333
                     /locus_tag="ACHUPM_004293"
                     /pseudo
     CDS             9..333
                     /locus_tag="ACHUPM_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(363..3275)
                     /locus_tag="ACHUPM_004294"
     CDS             complement(363..3275)
                     /locus_tag="ACHUPM_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFR0871901.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPKGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            complement(3332..>3530)
                     /locus_tag="ACHUPM_004295"
     CDS             complement(3332..>3530)
                     /locus_tag="ACHUPM_004295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871902.1"
                     /translation="GDMFTSEQHRCSNEKKEFQRRVYLRGDAFINLYTNFYSGIMTGL
                     QLLRHFQRIALPAYHPTFFIP"
BASE COUNT          880 a          948 c          868 g          834 t
ORIGIN      
        1 gttgaacgtg gtgtcagcgg cggacaggga taagccctcg tcaacagttc cgtcagcact
       61 gcgacagcgt tctttacccg gtcaaaacag cgttgcggtg caccacgcct gacggatgaa
      121 ctgcgtgctc agggttacca ctttaacgta aaaaccgtgg cggcaagcct gcgccgtcag
      181 gggctgaggg caaaggcctc ccggaagttc agcccggtca gttaccgcgc acatggcctg
      241 cccgtgtcag aaaatctgct ggagcaggat ttttacgcca gtggtccgaa ccagaagtgg
      301 gcgggagaca tcacgtactt acgtacagat gaagttcggt tacatcctgt cagcacagaa
      361 cctcacgctt tttgagcggc acgacggatc ccccccaccc agtatccgac agacttttca
      421 ctgcgttcgt aaaaaccctt cttccctccc agcatatcca ctaattccgg tgacatatcc
      481 tgcagataag tctgcaaatc atgccccaga tcttccggtg actgcaactc ataaaccgcg
      541 cgccacacat caccctgcct tattttaagc cccgggttaa gaatatactg tccgtggttc
      601 agcctcagaa aaagcttttt tccataagga tttttgctgt taacttcatg ttgagagagc
      661 tggctgctga tataactcct tcgtttgcgg cgttccggca gcaacgcttc aggaagatga
      721 accacacact ctgccagtcg tgccgcactg aaggcttccg aaaaaaacag agcacttccc
      781 agatgcgtgt ggaacagcgc ctgcatcagg atcaccagaa acattgccat ctgccggttg
      841 tccagtttga tgaggcgtcc ttccacctgc agtgacagac tttcaggttt cagcagggta
      901 tacaactgcg cacttttctg ttgcgcataa cggggcgtac tgaccgccgc tgacagaact
      961 tgatgataag ccgccagccc gtcattaccg gtcagcaagg gatctgcacc acgctccagc
     1021 agtaactgaa ccagtgcaat attacctgcc cgggctgctg acatcagcgg agtaagatta
     1081 aacggcgaac ggtgatcaac accataacgt tcaatatccc gcaacacggc aaccgggtta
     1141 ttcattgagt atggccggaa atacttcagc tccagtaact gcagagtctt cgttctgggc
     1201 tgccttgtgg gcttaaaatc ttcccggtac agtgcattat acagcgggaa cagggaataa
     1261 atcagactgt actccagcgc ctgcagttgt atggttttat cggctgtacc tgcgaggata
     1321 tgctgtttcc actgtcgtgc atcctctgcg gtgataatct gccatggcat ctcagatgtc
     1381 tgcaggatcc ggctacggat ggtgtccgcc tgctcctgct tcccctgttt ttcaagcaga
     1441 tgtgcggttt tctgccactc atcacggctg gaaatctctt cctgcagatt cagaggctct
     1501 tcctgatgcg tgagcgcaag cagactccac agaggatgct ccacctgctg ttcaacaaga
     1561 tacacattgt gtgtagcacg ggtgagcgca acatacagcg cgttggtaaa aaatttatag
     1621 atttccgctg aacgatcctg cctgtcccgc ggacgggaat aacgggcctc gccctcaaga
     1681 tcagccggtg taagaccctc gcaaatatca tcaaatgcct gccgggcagc gcttactata
     1741 ttgtaaagga tcaccgtttc gtattccagc cccttaacct cctgaaccga aaagaccagc
     1801 ggcgtactga accagcatcg cgcctgtgct ttctgttccg gatgcatcac aatgaccgct
     1861 acacgattcg aaagtgaggt tttggcattc agttcctgct ttgtctcttc cctgtcgtcc
     1921 agcaaccgga tggtcccttc tgcctgaccg cagcttctga caaaatggtg actttcccga
     1981 tcaatagcac taaagcggac ctgtttgagt ctcagcaacc ggttagccag tgcagtgacg
     2041 tggtgcccgt tacggtaatt cgcctgcagg atattaacgg ttgtgtcgtt tccctgttgc
     2101 tgccggaaaa agagactttt gagcgaactc catgagaaaa agctcggatg aactatctga
     2161 ttggcatcac cacagagtaa aaactgaccg ggatgacgta gcgttttcag taccagttgc
     2221 agttgaacag gcgtcatatc ctgaacttca tcaacaaaaa tgacatcata acggggagtt
     2281 gccaacgaaa gatactgatg actgagcaga ttactgtcat actgatgcga ctgtttcagc
     2341 catgcgatat aacgttcaaa caggacatag accgtcgggc ggtcctccat gccatagagc
     2401 gactgccgta tccccagtga cagatacgcc tcccgggaca gcggcccatt cccggaggca
     2461 accgcaccga tcacgcccct gaactcctca tacagggtat gggcagcccc cagtgcggcg
     2521 atagccctgt tgcggggcag ccagtcgctg aacgcactga aagtcacctc cctgcctttc
     2581 ggaatgcgca gagtttccag aaattccgtc agcgaaagaa agtcaatatt ctgtacctca
     2641 ctcccttccc ctgaagcgtc atagagagta cgcgcttttt ccaccagaaa tgaggaaagg
     2701 ctgagataca gtatatcacc agcagcctgc ttcatttttt ccagtaccag cgccgtctta
     2761 ccacttcccg ccgagccaac aatcaccagc ggtagcgggt gttcgtacag tgcctgctga
     2821 tcatcatcaa aggacagcat tttatccagg cggtggaaac gcccatgaga cggattgata
     2881 tacgtcagcg cttcagtcgc gatatcaaca ggatccggca ctggttgttg ttgtaagcgc
     2941 ccttcatcaa tcaccacgtt tctgcgaaga aaacgtgaag tgttataggc atgattgcga
     3001 atatattcca gaacaaggca gatagtttca ccgtggtagc gatacagcga aaacagtaat
     3061 cggtcccgga tactgagccg ggcgcggtaa agattggtgt caattttacg aacatcagcc
     3121 tgaacaaagt tatcggtggc aatagcctgt gcaaattttt tataacccgg gatcgtgtca
     3181 ggattcagag tatgataaag acagattttc atggctgccg gtttccgtcg tgcaaaaaac
     3241 atgctgattt tacgaaggaa tgccttactg aacaaccttg cccggaatac cccccgaaat
     3301 cagatggaac aatcagaaaa atgctctgca gttatggaat aaagaaggtg gggtggtacg
     3361 ccgggagggc gatccgctgg aaatggcgca gtagctgcag gcctgtcatg atgccggaat
     3421 agaagttcgt gtacaggtta atgaaagcgt caccccgtaa ataaactctg cgctgaaatt
     3481 cctttttttc attggagcac ctgtgttgtt ctgaggtgaa catatcacct
//
LOCUS       DAZPAC010000336         3517 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZPAC010000336 DAZPAC010000000
VERSION     DAZPAC010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.341"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..266
                     /gene="rutC"
                     /locus_tag="ACHUPM_004296"
     CDS             <1..266
                     /gene="rutC"
                     /locus_tag="ACHUPM_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR0871903.1"
                     /translation="VLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKN
                     YAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
     gene            274..1074
                     /gene="rutD"
                     /locus_tag="ACHUPM_004297"
     CDS             274..1074
                     /gene="rutD"
                     /locus_tag="ACHUPM_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR0871904.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            1084..1674
                     /locus_tag="ACHUPM_004298"
     CDS             1084..1674
                     /locus_tag="ACHUPM_004298"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR0871905.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            1685..2179
                     /gene="rutF"
                     /locus_tag="ACHUPM_004299"
     CDS             1685..2179
                     /gene="rutF"
                     /locus_tag="ACHUPM_004299"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR0871906.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            2200..>3517
                     /gene="rutG"
                     /locus_tag="ACHUPM_004300"
     CDS             2200..>3517
                     /gene="rutG"
                     /locus_tag="ACHUPM_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR0871907.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVH"
BASE COUNT          697 a          935 c         1034 g          851 t
ORIGIN      
        1 acgtgctgtt tgccgatgac ccaaaggcgc aaacccgcca cgttctggaa actatccgca
       61 aggtgatcga gacggcgggt ggcacgatgg cggatgtgac cttcaacagc atctttatta
      121 ccgactggaa aaattacgcc gcgattaacg aaatctacgc cgagtttttt ccgggtgata
      181 aaccggcgcg attctgcatt cagtgcggac tggtaaaacc tgacgcgctg gtggaaatcg
      241 ccacaattgc gcatatcgcc aagtgaggcc gcgatgaaac tttcactctc acctccccct
      301 tatgctgatg cgcccgtagt ggtgttgatt tcgggtcttg gcggtagcgg cagttactgg
      361 ttaccgcaac tggcggtgct ggagcaggag tatcaggtag tctgttacga ccagcgcggc
      421 accggcaata atcccgacac gctggcagaa gattacagta tcgcccagat ggcagcggaa
      481 ctgcatcagg cgctggtagc cgcagggatt gagcattacg cggtggtcgg ccatgcgctc
      541 ggtgcgctgg tgggaatgca gctggcgctg gattatcccg cgtcggtaac tgtgctgatc
      601 agcgttaacg gctggctacg aataaacgcc catacgcgcc gctgttttca ggttcgcgaa
      661 cgattactgt atagcggcgg cgcgcaggca tgggtggaag cgcagccgtt gttcctctat
      721 cccgccgact ggatggcggc ccgcgcacct cgccttgagg cagaagacgc gctggcactg
      781 gcgcattttc agggcaaaaa taatttactg cgtcgactta acgccctcaa acgcgctgac
      841 tttagtcacc atgcggatcg catccgctgc ccggtgcaaa tcatctgcgc cagtgatgat
      901 ctgctggtgc catcagcatg ttccagtgag cttcatgccg ccctgcccga tagccagaaa
      961 atggtgatgc gctatggcgg acacgcctgc aacgtgaccg accccgaaac gtttaatgct
     1021 ctgttactca acgggcttgc cagcctgtta catcaccgtg aagccgccct gtaaggaatt
     1081 gctatgaacg aagccgttag cccaggtgcg cttagcaccc tgttcaccga tgcccgcact
     1141 cacaacggct ggcgggagac acccgtcagc gatgagacgt tacgggagct ttatgccccg
     1201 ataaaatggg ggccgacatc agctaactgt tctccggcac ggatcgtgtt tatccgcact
     1261 gtagaaggaa aagaacgtct gcacccggcg ctttccagcg gcaatctaca aaaaaccctg
     1321 accgcgcccg tcaccgctat cgttgcctgg gacagtgaat tttatgaacg gttaccacta
     1381 ctgtttcccc acggtgatgc ccgcagttgg tttacctcca gcccacaact tgccgaagaa
     1441 acagcgtttc gcaacagctc catgcaggcg gcctatctga tcgtcgcttg ccgtgcgctg
     1501 ggactggata ccggcccgat gtcgggtttt gaccgtcaac acgttgacga cgcctttttt
     1561 gcgggcagca cgctgaagag caatctgctg attaatatcg gctatggcga tagcagcaaa
     1621 ctttttgcgc gcctgccacg tctgtccttt gaagaagcct gcgggctgtt gtaaggagca
     1681 aactatgaac atagttgatc aacaaacctt tcgcgatgcg atgtcctgta tgggcgcggc
     1741 ggtcaatatc atcaccacgg acggtccatc cgggcgcgcc gggttcaccg ccagcgccgt
     1801 ctgcagcgtg accgacacgc cgcccacatt actggtgtgc ctgaatcgtg gggcgtccgt
     1861 ctggccggta ttcaatgaaa atcgaacgct gtgtgtaaat acactgagcg ccgggcagga
     1921 gccgctttca aatctttttg gcggcaaaac gcccatggaa caccgctttg ccgccgcccg
     1981 ctggcagacg ggtgtgaccg gatgcccgca actggaagag gctctggttt cgtttgactg
     2041 ccgcatcagc caggtggtga gcgtcggcac ccacgacatt ctgttttgcg ccatcgaagc
     2101 gattcatcgt cacaccacac cctacgggct ggtgtggttc gatcgcagtt atcacgcgct
     2161 gatgcgccct gcttgttaac ctccttaagg agacagctga tggcaatgtt cggttttcct
     2221 cactggcagt taaaatcgac ctctacagag agcggcgtgg tcgcgccgga tgaacgattg
     2281 ccgtttgctc agacggcagt aatgggcgtt caacacgcgg tggcgatgtt tggcgcgacg
     2341 gtgttaatgc cgattctgat ggggctggat cccaatcttt ccattttaat gtcggggatc
     2401 ggcacgttac tgtttttctt tattaccggc gggcgcgtcc ccagttatct cggctccagc
     2461 gcggcttttg tcggcgtggt gattgccgcg acgggcttta acggtcaggg cattaacccg
     2521 aatatcagca tcgcactggg cgggattatc gcctgtgggc tggtttatac cgtcattggc
     2581 ctggtggtga tgaaaattgg tacacgctgg attgaacgac taatgccgcc ggtggtgacg
     2641 ggtgcggtgg tgatggcgat tggcctgaac ttagcgccta tcgcagtgaa aagtgtatcg
     2701 gcttcagcct ttgatagctg gatggcggta atgacggtgc tgtgtattgg gctggtggcg
     2761 gtgtttaccc gtggcatgat ccagcggtta ttgattctcg taggtttaat tgtcgcctgt
     2821 ctgctgtatg gcgtgatgac caacgtttta gggctgggaa aagccgtcga ttttactctc
     2881 gtcagccatg ctgcctggtt cggtttgcca catttttcca ctcctgcttt taatagtcag
     2941 gcaatgatgc tgattgcgcc cgtggcggtg attctggtgg cggaaaactt aggtcatctc
     3001 aaggcggtcg ccgggatgac cggacgcaat atggacccgt atatggggcg agcattcgtc
     3061 ggtgatggtc tggcaacgat gctttccggc tctgtcggcg gtagcggcgt caccacctat
     3121 gctgaaaata tcggtgtgat ggccgtgaca aaagtctact cgacgctggt gtttgttgct
     3181 gctgccgtca tcgccatgct gttgggcttt tcgccgaaat ttggcgcgct gatccatacc
     3241 attccggccg cggtgattgg tggtgcatca attgttgtgt tcgggctgat tgccgtcgca
     3301 ggcgcaagga tctgggtaca aaaccgtgtc gatttaagcc agaacggtaa tttgattatg
     3361 gtcgcagtaa ccttagtgct gggcgcgggt gattttgctc tcacgctggg cggttttacg
     3421 ttgggaggga tcggtacagc cacctttggc gcgattttac tcaatgcgtt gttaagtaga
     3481 aggttggttg acgttccacc gccagaagtt gttcatc
//
LOCUS       DAZPAC010000337         3502 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZPAC010000337 DAZPAC010000000
VERSION     DAZPAC010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.342"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..301
                     /locus_tag="ACHUPM_004301"
     CDS             <1..301
                     /locus_tag="ACHUPM_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871908.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            390..2258
                     /locus_tag="ACHUPM_004302"
     CDS             390..2258
                     /locus_tag="ACHUPM_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFR0871909.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2295..>3502
                     /locus_tag="ACHUPM_004303"
     CDS             2295..>3502
                     /locus_tag="ACHUPM_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFR0871910.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     RNQLLKSLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTLEYIDN
                     TLITFSNEEIIKCQIAIQSIINKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALANIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQ
                     KLSQNKINKF"
BASE COUNT         1115 a          635 c          674 g         1078 t
ORIGIN      
        1 acgtgtagaa ctgtattacc gctttaacca tgaagaagaa tattaccctt ctcaccgttt
       61 tatgcttgtt gttgatatga agacagtaat ggttttttca caacgagctg aatcactggg
      121 gttatactcg aataatcgct tgcttatcaa tataattgcc gaacatatcc atagtgatat
      181 atatctaacc aagtatcagc aacaatatcc agatgccaat gcattaataa atactttgca
      241 tgaatctggc aatgatatgg tgcaggatga aaaacatttg agtgatagca aaaaccgtta
      301 atattttcct ttcattgtga tcgtctgtag gcgatcacaa tctatttgtc ttttataacg
      361 tctaaacttt tttatgcaaa ggtgtattta tgattttttt taacgatata aagcccactg
      421 gttggttaaa gaataagatg gaatggtata tgaacggtca tataggagaa ctggataaat
      481 tagttcccca tcttattacg gagcataaaa tatatggaag agatcgtata actccacgca
      541 ctgtatctgc tgaagatggc gttattaaaa aacagaataa tcccgatgat aacatgatgc
      601 aatattattg gtgggatagt gaaactcaat cgaattggaa ggatggatat tgccgcagtg
      661 cctacttgtt agataataaa gaatggcagg aaaaagcgag cgatttatgt ttggaactta
      721 tcgatacgca agatgatgat ggctacattg gaatttatga ttcatcatta aaatttaact
      781 gtaaagcaga aaacggagag ttctgggcac aagctactgc cttgcgtttt gtgcttggat
      841 gttatgagtc cacccgtaat gagagtttat taacttctct tacccaagcc gcacaatgca
      901 ttatggctgg ctaccctaaa gaaacgtcac atcctttcgt tgtggaacag ggttttgctg
      961 gacatagcca cggattaaca ataacagatg ttttctaccg cctttatgaa ataaccaata
     1021 acattgaata tctctcatat tgcttgtggc tgtatgaaga ttattccgca ggatgcgtgt
     1081 cggagcaaga tctgcaatat aaaaatgtca tagaccctgg ttaccttctt aaaggtcatg
     1141 gcgtacatac gtatgagcat attagggcat tagccatcgc agcgagccaa cggccgcagt
     1201 atcaaactgc attagatatt tttttatccc agttgaaata ttatcttaca ccatctggcg
     1261 ctcccattgg tgatgagtgg attacaggac gcacagctaa tgccacttat acaggctatg
     1321 aatattgttc tgttcatgaa ctatttagca cttatgttat gttaataaaa ctgaccacta
     1381 atattggtct ggccgatgat gctgagtggc tattctataa tgctagtttt ggcatgcagc
     1441 atcccatact tcacgcgata atgtactgta agactgataa ttgttatgaa gcaaatgaaa
     1501 ggaaacatcc tgacagtaat cagtttaata ccagatataa atactcccct actcatgagg
     1561 aggctgctgt ctgctgcgta ccgaatacag caagaaccat accggctttt gtcgaaaata
     1621 tgtttcaaga aaataacaat ggttttactg cattatttta tggcccgtgt gagttttatg
     1681 gcacatttaa taatgttgca attaaaatta cacaattaac tgaataccct catgatttaa
     1741 gcgtaaaatg tctcattgag cctgagtcat ctgttcgttt tgctctcagt ttccgttacc
     1801 ccggctgggc aaaaatgatg attattaatg gtgttacttt tactaaaaat gatactcaaa
     1861 actcacttat tattttagat agaacatggc aacatcatga tgtgattgac attaaattta
     1921 tagctgatat acagtttaat actgacttat gtggtgacac ttatatttcc agaggtcccg
     1981 ttttatatgc aattgagctt gagagcgata ttttaatcaa aaaaaacctg ctgaacggga
     2041 aatattttga ctcaggatac ataccctgta gtagagctga tgaatctatc cagttctctt
     2101 atgctgatat ggaatctttt tcatattcaa cctcaccaga gggcaaacaa tacattgaag
     2161 gctgttttta tttaaacaag actaaaatag tcagaaaact tataccattt ggtcaaacca
     2221 ttcttcgcaa agtaactttc tccaatatag agagttaata ttttcgagtg ttataattaa
     2281 ttaaagggat ttctatgtac cttactgata atattatcaa actgtcgaat aaaataggtc
     2341 agcgtgttta tactgttgtc tctgaaatgt cgattgaagc atggataaca aaagaaccgc
     2401 agccttttgt ttatcgccgt agcggcgctt accagaaact caccattggc tcagactggg
     2461 gtcaactgtt tgactgtgcc tggatgcgag tttatggtaa acgtccagca gataaattta
     2521 gacacaaact cgtcgctctc gttgatatcg gtggcgaagg tcttattgtg gataagaatg
     2581 gttcacccat ctgcggcatc acgaataaag catcatctta tggcgtaccg ccagataaac
     2641 caggtaaatg ggtggttgac ctttctctcg ttagtgaaaa tgacgaggtt gagttttgga
     2701 ttgatgctgc ctgcaacgat ttatttggct atgttactaa tggagggatt attactgatg
     2761 ttcatatcgc gacacgtaat caactactga aatcccttta ttatgatatt gaggtgcttt
     2821 tcgactggat taatgatggt cagaagtttg aatccataca tcctaaaggt atcaccccag
     2881 aacaatttat tgccaaaaga agtgagcgta ctgacgaaat cataagtact ctcgaatata
     2941 ttgataatac gttaattacc tttagcaatg aagaaataat taaatgtcaa attgcaatcc
     3001 aatccataat caataaaaac aataatcctt cagagttcag gattatggct actggtcatg
     3061 cccatttaga tatagcgtgg atgtggcctt taagagaagg acgcagaaaa gcaataagaa
     3121 catttgccac cgcccttgca aacattgaaa aatatcctga ttacattttt ggcgctagtc
     3181 agtaccaact ttttcactgg ataaaaaaag attatcccta tttcttcgaa aagttaaaaa
     3241 aacaaatcgc cgcaggccgc tttgagttac aaggttgctt ctgggtcgaa tgcgatttaa
     3301 atctggtaag tggtgaatcg ctgataaggc aaatcatgca tgggacacga ttcacactgc
     3361 agaatttttc aaaaaagata aattatgtct ggcaaccgga tgtctttgga ttccccgcaa
     3421 cgcttccaca aattctcaaa aaatcaggca ttaactatat tgcatcgcaa aagctctcac
     3481 aaaacaagat taataaattt aa
//
LOCUS       DAZPAC010000338         3448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZPAC010000338 DAZPAC010000000
VERSION     DAZPAC010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.343"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..279
                     /locus_tag="ACHUPM_004304"
     CDS             <1..279
                     /locus_tag="ACHUPM_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR0871911.1"
                     /translation="YTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWK
                     NRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA"
     gene            620..2272
                     /gene="ushA"
                     /locus_tag="ACHUPM_004305"
     CDS             620..2272
                     /gene="ushA"
                     /locus_tag="ACHUPM_004305"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
                     /protein_id="HFR0871912.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIREHSPLDAASYEPKGEVSWQ"
     gene            complement(2283..2453)
                     /locus_tag="ACHUPM_004306"
                     /pseudo
     CDS             complement(2283..2453)
                     /locus_tag="ACHUPM_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2586..2705)
                     /locus_tag="ACHUPM_004307"
                     /pseudo
     CDS             complement(2586..2705)
                     /locus_tag="ACHUPM_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2779..3327)
                     /locus_tag="ACHUPM_004308"
                     /pseudo
     CDS             complement(2779..3327)
                     /locus_tag="ACHUPM_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
BASE COUNT         1000 a          747 c          795 g          906 t
ORIGIN      
        1 tacacacagc ggcttaagga agccggatta ctggcatcaa caggaagtac aggcgactcg
       61 tatgacaacg cgatggcgga gagcatcaat ggtctttaca aagcggaggt aatacaccgt
      121 aagagctgga aaaaccgtgc agaagtggaa ctggccacac tcacgtgggt ggactggtat
      181 aataatcgac gattgctgga aaggctgggc cacatccctc cggcagaagc agaaaaagct
      241 tattatgctt ctatcggaaa cgatgatctg gcagcctgag ttcacagata aaacattctc
      301 caggaaactt ggggcggttc acgtcagtat cagaccatat taattgctga aactgataaa
      361 catcaaacct tatagaaggg tgaatgctga aagggggcta tggtgatcga cgtgatgaca
      421 acgacaggta agaccgtgac ctgataaacc tgaattgtgt cctagacata aagtccgtga
      481 agggaataag gctcaggtca gcgaatctta tagtggcccg cagctctgac tgcaataagg
      541 ccggatacag agcagcaact actcgtcgat gtcagaacaa cggaagcctt gcaaacgggg
      601 atgcgttcat acaggtacaa tgataccgtt aaaaaaaaac attacgctaa taatgttcac
      661 cctgtcatta ctgacaggaa atcctgctat agcgtatgaa acagataaag tatataaaat
      721 aaccgttctg catacaaatg accaccatgg acacttttgg cgtaacaatc atggtgagta
      781 cgggttatcc tcccaaaaaa ctctggtgga caatatccgg cagaaagtaa taaataatgg
      841 cggaagtgtt ctattattat ctggtggtga tattaacacc ggtgtacctg aatcagacct
      901 tcagaaggct gaacccgata ttcgtggaat gaatctaatt ggttatgatg caatggccgt
      961 cggtaatcat gaatttgata atcctctaaa tatattacgt cagcaggaaa aatgggccac
     1021 atttcctttc ctttctgcta acatatatca aaaaagtaca gggaggcgtt tattcagtcc
     1081 atggaaaatt ttcatccgtc agaatttgaa aattgcagtc attggcttga cgacagatga
     1141 tactgcgaaa acaggaaatt ctgaatattt tactgatatt gagttccgtc aacctgctgc
     1201 tgaggcgagg agtgtcattg atgaacttaa tcagcaggaa aagccagata ttattattgc
     1261 ggcaacacat atgggtcact atgacaatgg cgagtcaggt tcgaatgctc ctggtgatgt
     1321 tgaaatggct cgcagtttgc cgactggctc attagcaatg attgtgggag gacattcaca
     1381 agctcctgtc tgcatggcca gcgataataa aaaacagtgg aattatattc cggggacaac
     1441 ctgtgtacca gataaacaaa atggcatctg gattgtacaa gcgcatgagt ggggaaaata
     1501 tgtcggtcag gctgattttg aattctgtaa cggtacaatg aaactggtta attatcagtt
     1561 acacccagtt aatctcaaaa tgagaattac cagagaagat ggaaaaacgg aattttcttt
     1621 ttacaccccc gaaattactg aagatccaca gatgttatcc ttactgacac cattccagaa
     1681 taaaggcaaa gcacaactgg atgtgaaggt tggggttgtg aacggacgtc tggaagggga
     1741 cagaagtaaa gttcgttttg ttcagaccag tatgggccat ctgattctga gcgctctgac
     1801 agaacgtatt gacgcagatt ttgctgttgt cagtggtggg gaaattcgag attccataga
     1861 gagtggtaac atcacataca aagatatact gaaagttcag ccatttggta atacggtagt
     1921 aagtattgat ttgacgggga aagaagtagc tgactaccta gctacagtgg cacagatgaa
     1981 accggattcc ggagcctacc cacaattttt gaataccagc tttgtagtca aaaaaggaaa
     2041 aatcgaaatg ctaaaaataa aaggtaaatc ggttgacctt aacaaaaaat atcgtatgac
     2101 aacatttagc tttaatgcta ctggtgggga tggctatcca cgtattgata atagacccgg
     2161 atacataaat accggattta ttgatgctga agtacttatc gaatatatcc gggaacattc
     2221 accgctggat gctgcttcat atgaaccaaa aggtgaagtc agttggcagt gataacgtaa
     2281 ttgacgatca tcacgtgttt tttgctgaca gtcgctgtga ctttgtgaaa gtagtctctg
     2341 cggatgtttg cgatatggga atgcagttgc tgtattttgt gtttctgctt ctgccagttg
     2401 ttgctgaatt taaccttgcg gcttaactga tgttggagcg ctgccagctt cttcgtcaat
     2461 ttcgctgatt aaagtttatt cattatggca tgatctgcgt aagcggctcg tcagaaccgt
     2521 attgatattt actaagagct cagatcaact ttccagggca acagatcgcg tacccggttt
     2581 gccggtcagt cctggatatg ctcaatgacg taacgcagcc atttttctgg ctccacattg
     2641 ttcagacggc atgtgccgat cagcgagtac aacaccgccg catgttcgcc accgctatcg
     2701 gaaccccgcc ggagagagtt tcactctccg gctgctcacg gaaccgtacg tgacactctc
     2761 gcgtcatacg gctcttgatc actcattcga tgcacgtagt aaataccagt gggcaaatag
     2821 cttcggatac tgtttcctta ccagcgccag ctttcgctga gccttccggt tcgaaagtct
     2881 gtatttagac tgcatccact taagcaaacg tgactgcatt gcactccaca gtcgatagct
     2941 gaagtttctg gaccagaact ttccatagta ctcaatccag cctctcacta tcgcattgta
     3001 gcgcctcgca aaatccagca aactctcagc tgttgagcga tgtatacgcc acttcttgat
     3061 tgtttctgtt attttgcaca ttgctgcatt tgatgcaccc ggcatacatt ttctgtacaa
     3121 ttcgcctttg aaattcttca gagtacgcac tttgaagtca tatccgagaa aggagaaact
     3181 cgtagcgacg tttctccttt taaacgtgtc aatgtaggcg atattcgtct tccctgcgtt
     3241 caggaccaac ccgacttccg agaagcgcac cgataacctg ttcttcagcc ttgttgcatc
     3301 actcattcgt gaacaatgta ctacaagtcg agtcacccga gggaacctcc ccctctggtg
     3361 ctcacggaac cggacgtgaa cctctcagct catccggctc ttctcattga ggcctgtccc
     3421 catagtaacg gccagtgtgc aaacaacc
//
LOCUS       DAZPAC010000339         3437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZPAC010000339 DAZPAC010000000
VERSION     DAZPAC010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.344"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            240..668
                     /gene="uspC"
                     /locus_tag="ACHUPM_004309"
     CDS             240..668
                     /gene="uspC"
                     /locus_tag="ACHUPM_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFR0871913.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(675..2099)
                     /gene="otsA"
                     /locus_tag="ACHUPM_004310"
     CDS             complement(675..2099)
                     /gene="otsA"
                     /locus_tag="ACHUPM_004310"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFR0871914.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(2074..2874)
                     /gene="otsB"
                     /locus_tag="ACHUPM_004311"
     CDS             complement(2074..2874)
                     /gene="otsB"
                     /locus_tag="ACHUPM_004311"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR0871915.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(3041..>3437)
                     /gene="araH"
                     /locus_tag="ACHUPM_004312"
     CDS             complement(3041..>3437)
                     /gene="araH"
                     /locus_tag="ACHUPM_004312"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR0871916.1"
                     /translation="RNTLAIGGNEEAARLAGVPVVRTKIIIFVLSGLVSAIAGIILAS
                     RMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKISYVVAGILILGTVENAMNLLNIS
                     PFAQYVVRGLILLAAVIFDRYKQKAKRIV"
BASE COUNT          878 a          849 c          802 g          908 t
ORIGIN      
        1 caacttaatg ttaatgaaat gttttacata cattaaccac ttgtttaatg cggagaatca
       61 gtacactatt ttcttataat tacatttgaa attatatgat caccgggagt gattgattaa
      121 ttgtctttac taataatcag actaatattt acctgtttga ccgagttggg attgcgtcgt
      181 ttctccatta ggagtaaagc tttaatgtca cctgaagttc acagaataag gaacaggtta
      241 tgagctatag caatattctt gtcgctgttg cggttacacc ggaaagtcag caactgctgg
      301 caaaagcggt atctatcgcc aggccagtaa agggacacat cagtttaatt actctcgctt
      361 ccgacccgga aatgtacaat caattagccg cgccgatgct tgaagattta cgcagcgtga
      421 tgcaagaaga aacgcaaagc tttcttgata agttcattca ggatgcgggg tatcccgttg
      481 acaagacgtt tattgcctac ggtgagttaa gcgaacatat tctggaagta tgtcacaaac
      541 atcatttcga tttggtgatt tgcggtaatc acaatcacag tttcttttcg cgagcatcct
      601 gctcggcgaa aagagtgatt gcctcaagtg aggtcgatgt gctgttagtt ccacttacgg
      661 gagattaacc gctcctacgc aagctttgga aaggtagcaa ctttatcgcg ctgctggctt
      721 tccgcgcttc gcggaactat ctgctttagg tcgctaatga agcactcctg ccagtggtta
      781 atatcgtttt tcacgataac gtccagcatt tctgcatgac gggaaatacg ttccgccagc
      841 gacatagtca atgcacgatc cagcgcagct gcaacttcgt cacgatcgta ggggttaaca
      901 attaacgccg acgttaactc gtttgccgct cccgcaaatt gcgaaagaac aagaacgccc
      961 gggttggctg ggtcctgagc agcaacatac tcttttgcta ccaggttcat cccgtcacgc
     1021 agtggcgtca ctaagcccac gtcagagtag cggaatattt tcatcagtaa tttacggtca
     1081 aaatgctgat tcaaataata aagcggcgtc cagcctaatt gcccgtattt accattaatt
     1141 cgtccagctt cattttcgag ctgatgacga atatcctgat aggcttgcac atcaccacgc
     1201 gacgttggtg caatctgggt ataacgaatt ttaccatgat gctgcggata tttttccagc
     1261 aacgcttcat aggcgagaaa acgctctggc aaacctttgg aataatccag ccgttcgaca
     1321 gaaaagatat tttgtacgtt tttcagttcc gctttaagtt gcgccagttt tggcggcagt
     1381 ggcccggcag cctgtttggc tatttctttc ggttcaatgc cgatcgggta gacttctgtt
     1441 cgaaatgctt tgccccaggc tgtatggctt tttgcgctac gtgtcgtgac gcgggtcagg
     1501 ttagaaagac aatccaggaa cgccagacga tcgttttctg tctggaaacc cagcaaatca
     1561 taatcacaaa gctgttcaag caaggtgtca tatgtcggca gcgcgttgaa gatttccggt
     1621 gtcgggaaag gaatatgcag aaagaaacca atgcgattat tcactccccg tttgcgtaat
     1681 tcatgcgcaa atggcaacag gtgataatcg tggatccaga taatgtcatc gtcttgcaac
     1741 agcggcagta atttatctgc cagcaacgca tttacgcgta gatagccgtc ccaggcagga
     1801 cgctgaaatt gcaccagatc gagccgataa tgaaaagcgg gccagagaac ggcattggag
     1861 aattggttgt agtattcgtc aaggtcctgt tcgctgaggt taaaagaggc ccacgtaatg
     1921 ttaccttttt tcaccttttt tagcggctga tcctcattcc ctgtttcacc actccagcca
     1981 aaccacagtc cgcctgcggc tttcagtgcc cccagtatgc caacggcaag gccaccggca
     2041 ctggcggcgt gctcgtctgg tggtgcaatc cggttagata ctacgactaa acgactcata
     2101 gtcatcactc ctgttatttt ctcttttttg ttgtaatgcg gtggttatca tttcaagcca
     2161 gctccagaca tccggcacac ccgccagtcg ccatgatgcc tgagttgcac ctgtgccaat
     2221 ttttactgac attccgccca gtcggttaac gactgcgaag ccagattcat cggttaaatc
     2281 atcgcccaga aatacgggcg ttcgcccgat aaagggagct tcctgcataa aagctgcaat
     2341 tgcctcacct ttactggtac ctctcggttt gatctcgaca acacactttc cctgctgtaa
     2401 cgccatttgt ggccagatct gagtaatacg ttgcgctaat gtcattaatg cgtcttcatg
     2461 ctgcggagcc tgacgataat gcagcgcaaa agccatccct ttcgcctcca gctccgcgcc
     2521 gggatactga gcgatgactg tatgcagttg cacgctaata tcacgcgcaa tcgcatccgg
     2581 cagatgaacg atatgtgttt taccattgat gtcacggcgc tccgccccat gcacgcccgc
     2641 taacgggaag cgataaggtt ttgccagtgc gtcaagctcc accattgagc gccctgatat
     2701 caatgccaat gcaccatcac ttgcggttgc cagtagctgt agtccttgca gaatattgtc
     2761 aggcacgacg acctgatcgg gatgcggttt gatttccgcc agcgttccat caagatcaaa
     2821 aaaccaggca tatttcgcgg atagttcagg ggtttcggtt aacggttctg tcacccggct
     2881 ctcctccttc tcttttctta ttgttttgcg cagaacgcgc tttacagaca tgtaagtata
     2941 gacagtgtga cgggggtcgc catttgcaat gaagaacagc caaccgttgt ggcggctggc
     3001 tgtggtggga aaaaacgtta aattgttgtg gaaaaaagca tcagacaatg cgtttcgctt
     3061 tttgcttgta acggtcgaag atcaccgctg ccagcaggat taagccgcga accacgtact
     3121 gcgcgaaagg agaaatatta agcaggttca tggcgttttc cacggtgcct aaaattaaga
     3181 tacccgccac cacatatgag atttttccga tgccaccttt cagagaaacg ccacctaaaa
     3241 cgcaggcgga gataacaata agctcataac caatcgacgt cattggctgc ccactagtca
     3301 tacgtgaagc cagaataatt ccggctatcg cagataccag gccagagaga acaaagataa
     3361 taattttggt gcgaacaacc ggtacacccg ccagacgcgc agcctcttcg ttcccgccaa
     3421 tcgccagggt gttacga
//
LOCUS       DAZPAC010000340         3416 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZPAC010000340 DAZPAC010000000
VERSION     DAZPAC010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.345"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHUPM_004313"
     CDS             complement(<1..312)
                     /locus_tag="ACHUPM_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFR0871917.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHUPM_004314"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHUPM_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR0871918.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHUPM_004315"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHUPM_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR0871919.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT          932 a          879 c          739 g          866 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccaaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc caaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atgtttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata aagtta
//
LOCUS       DAZPAC010000341         3382 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZPAC010000341 DAZPAC010000000
VERSION     DAZPAC010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.346"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..2253)
                     /gene="katE"
                     /locus_tag="ACHUPM_004316"
     CDS             complement(<1..2253)
                     /gene="katE"
                     /locus_tag="ACHUPM_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR0871920.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKI"
     gene            2436..2699
                     /gene="cedA"
                     /locus_tag="ACHUPM_004317"
     CDS             2436..2699
                     /gene="cedA"
                     /locus_tag="ACHUPM_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR0871921.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            2988..>3382
                     /locus_tag="ACHUPM_004318"
     CDS             2988..>3382
                     /locus_tag="ACHUPM_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310463.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871922.1"
                     /translation="MFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFISTGQC
                     DCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGSEFRI
                     RDINENFVGMDFTCEFRPEKMTNLINIKN"
BASE COUNT          851 a          766 c          840 g          925 t
ORIGIN      
        1 aattttgtca atcttaggaa tgcgtgacca cacgcggtgt gctgccatca gcgttagcag
       61 ttcatccata aaactaccgt cagcgctgtc agcttccaca atcccttctt caccctggtc
      121 agcgaccttg attgttgctt taaacttgcg cgcgtctccc gccagcgcaa tcggtttaag
      181 gtgtttgtag gcttccatca ggtagtagtt ggcatcgccg ttgtcagcga tatccgcgat
      241 attgccgcaa gggacaatga ccgcatcgac cgtcagcgaa ggtgcaccgg caaaggtagc
      301 ggctataggc aacaccgtac cgtcatccgc agtcacttca cccattcggg agtagagcag
      361 tttggcatga acgcctttgg ccttcagggc cttgagaatg gccagaaggt ctgccgatct
      421 cacttcatca ttaagtaaaa tcgctaccac gcgacctttc acatcaccgt caggaatggc
      481 gtacaaactt aaggatggat cctttttcag accgttgacg tccggaggtg gggtgatatt
      541 cagctggtcg tcagtcagtt cgataccgag atttttcgcc accgcctggg ccagagtgag
      601 atcaatatgc gccagctggt caacaacgcg ctcacgaata tacggacgaa cgactttgct
      661 taactcaaaa ctgaaaccat cgacaatatg gcgctgctcg aatggcgtct gacttagcca
      721 gaacagacgc ggatgggaat aatattcgcc aaacgatgga ctgcgctcac gaactttatt
      781 tccttccacg cgttcctgat atgattcaaa accgccgcgt ttcggtcccg gcggtgtttc
      841 gcgcggccag ttatcgttga tcgagttcgg ttcgtaattc gccgggttag tgtcgatccc
      901 catacgatgc atgccatcac gctggaagtt gtggtaaggg caggtcggac ggttaatcgg
      961 aatctcatgg aaattcggcc caccaagacg actgatttgt gtatcggtat aggagaacaa
     1021 acgtccctgc aacagcggat cgttggtgaa gtccagacca ggaacaatat gacctggatg
     1081 gaatgctgct tgttcgtttt cggcaaagaa gttatccgga ttgcgattga gcaccatttt
     1141 gccgacacgc tgaacgggca ccagttcttc cgggatcagt ttggttggat cgagaagatc
     1201 gaagtcgaac ttgaattcat cttcttcagg aatcaactgg aagcccagtt cgtattccgg
     1261 aaaatcgcct gcttcaatgg cttcccacaa ctcgcggcgg tggaagtccg ggtcacgtcc
     1321 ggtgagtttt tgtgcttcat cccaaacgag tgaggcttta cctgccagtg gtttccagtg
     1381 gaaacgtaca aacgttgcct tcccttcggc attaatcagg cggaaggtgt gaataccgaa
     1441 gccttccatg gtgcggtaac tgcgggggat gccgcgatcc gacatcgccc acatcacgtt
     1501 gtgcagagtt tcaggttgca gagaaacata atcccagaaa gtatcgtggg cactttgccc
     1561 ttgtggaatt gcccagtgcg gttctggttt taccgcatga acaaaatcgg ggaatttatg
     1621 cgcatcctgg ataaagaaga ttggcgtgtt attgccaacg aggtcaaaaa taccctcttc
     1681 agtatagaac ttggtggcaa aaccacggat atcacgcacg gtatctgcag agccagcgcc
     1741 accctgaacg gtagagaacc taacaaatac tggggtgatt ttgttcggat ctgagaggaa
     1801 atccgctttg gtgatatcgc ttaagctttt atatggctgg aaataaccgt gagctgctga
     1861 tccgcgtgca tgaacaatac gttccgggat gcgctcatgg tcaaagtggg tgattttctc
     1921 gcgcagaata aaatcttcca gcagcgttgg accacggtta ccggcacgca gtgagttttg
     1981 atcatcggcg atgcgcacgc cctgattagt ggtcagcgca taattttcac tgcctttgcg
     2041 tacgtcttcc agagaattaa gtttttcgtt acgtgtatca ggggctttca ggctcccagg
     2101 ggcggtaggt tgtgcacctg gcggtgttgg ttcagccgct ggacgatgag atccgtcctc
     2161 aggtgccagt gagtccatcc ccggtttcgc ttcgctggaa tcgtgtagtg gtgactggtg
     2221 ctgatgtagg ttcttttcgt tatgttgcga cattgaactc gtctccttaa tttattactg
     2281 aaagggccgc tgttttggtc aaaaaactct ctaactatag accaccagcc agatcccgct
     2341 tcggagacta ttctggaaac ggtgttatga aatacacgca aagcgggacg tacaggggct
     2401 aaatcggcta aactacagtt tttctaattt ttaatatgcg tttagtgaag ccagtaatga
     2461 agaaaccgct ccgccagcaa aaccgccaga ttattagcta tgtcccacgc acggaacccg
     2521 cgccgccaga acatgcgata aagatggatt cgtttcgtga tgtctggatg ctgcgtggca
     2581 aatatgttgc gtttgtactg atgggagagt catttctgcg ctcaccggcg tttactgtgc
     2641 ctgaatctgc ccaacgttgg gcaaatcaga tccgccagga aggggaagtg actgagtaag
     2701 gattacatac ttcccggttt tgccgggagt tacatgacct gaaattctgc atttcgttgc
     2761 tacattcatt caaaccacct gtaacaaaaa gctgcctttg acgagctatt gctataaata
     2821 agtgatcttt tttctttaat tgtttattta ttttaaaatg gttaagtttt gctaatcatg
     2881 atgagctatt tttgatagtt catcctgtgg cgtcgctctt aaacttatat tgtaagtgac
     2941 ttagatttaa atgagataat aattttgtta acaatatggg gcgggagatg ttttctacaa
     3001 acttaataca gagtaattat ggtgatttaa atattaaaag cctggctttc gactctttta
     3061 aagaaaggct tgagtctacc atggcggcac tcactttctt tatatcaacg ggtcaatgtg
     3121 actgcgatga aatagcagag tcgaacttca atcgtatgat tgcctatatg tctaacatta
     3181 actatgatgc ctcaaagcca ggagctccag cacttagttt tgatacgtat ctgcaggaca
     3241 atgttaaata tagagtgata attaataatc tttatggcag tgagtttcgg ataagagata
     3301 ttaatgagaa ttttgttggg atggatttta cctgtgaatt ccgacccgaa aaaatgacga
     3361 atttgatcaa tataaaaaat ag
//
LOCUS       DAZPAC010000342         3350 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZPAC010000342 DAZPAC010000000
VERSION     DAZPAC010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.347"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHUPM_004319"
                     /gene_synonym="dgcC"
     CDS             complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHUPM_004319"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR0871923.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHUPM_004320"
     CDS             complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHUPM_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR0871924.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHUPM_004321"
     CDS             complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHUPM_004321"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR0871925.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHUPM_004322"
     CDS             complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHUPM_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR0871926.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          798 a          883 c          785 g          884 t
ORIGIN      
        1 ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc gcccacatca
       61 tgaccccagg tatcgttgat gctcttgaaa tggtcgatat cgataatcag taacgttgca
      121 tcgcgattat gccgccgaca gttatcaaat tcattgcgta acatagtttc ccaatgacgt
      181 cggttataca cgcccgtcat gccgtcgcga gtactcatga cctgcaacct gcgtttatgt
      241 tccgccagtt tggttgccgt ctggtagctg acccagccaa acagcagagg ataaacgaca
      301 ataatgggaa gggagagcca ccattccagc ggcgcactat tgaacgacac ggtaatgccc
      361 gtaagctcga gggtgacaag gcaggaaacc accatcaaca ccagacccgc gacaaacaga
      421 cgggggccgc ctgcccccat caaattcaga cacataatca tcaacatagc ggtggaaggc
      481 agcacgttta cgcccattac gcctacccac attcccgcta atactgcatc ggtttttaag
      541 ttgtaaattt cccggctaag cggatcgacg gccctgctcg ctatctgcca ggctaaatgc
      601 ggccagacga acgcccagcc gaccaacacc agccaccacc agcccggcgg cgggtgcgaa
      661 accagcgttg aagcaatcgg taagaacatg ccagccaggc caaccgcacg gggtagtctg
      721 acgcgacggg cgaagcgcag cccggaccgc tgatgttcgt tttgaggagt taaaggaggt
      781 tcctccccat gcgccgccgc ttttttgaaa aagttttcat cattcattat ttttgggaac
      841 attcagaaac aattttccca aattatagag acggggctaa aggcagaagt atgaaatttc
      901 gggtgagcca ctttcgtagc tcacccgtta gcactatcag gtatcaggcg gctttcttga
      961 ggcaggcact cataaactta ttacgatcat cacctttcag agattgttgc gttgcttgat
     1021 tattacattc gcgcatcttt tgctgctgtg gcgtcaaact tttttcacca ggcgcagact
     1081 tgctgttctt caggcaatca ctcatgtagg tcttacgagc atcccctttc aacgcctgcg
     1141 ccgtcgcctg ctgattacag gaggtcatac gctgttgttg tggggttaag gttctctcgg
     1201 cagcgccgac ggtggttaaa aaaaccagac caaaaagcaa ggtaaccagt aatgttattt
     1261 tcatagcacc atccctcttc atgttttaac catgagcgtt tgcgcccgtg atctgccatt
     1321 aagtctggtt gctaacagca aaaaaaccac ccggcagcga aaattcactg ccgggcgcgg
     1381 ttttatttca gccccagagc ggctttcatg gtgtagaaga gatcggtctg gtcggtcagt
     1441 ccaacaacat tggcggcatg cggaccatac gccgcaatac gcaactgact gccggtatgt
     1501 tcttgtgaat cctcttcgga attcccgtaa ctcatcacca tcactgcgcc atctttagta
     1561 tttagcgcct gagtgaggcc cggcgctttg gtatccggcg caacaacctg gctggcgtgg
     1621 gcgtgatcag cggtgactat gaccagcgtg ttaccctcct ttttagcgaa ttccagcgcc
     1681 cgttgtacgg cttcatcgag atcgaccgtc tcgccaattt gcccacaagg attcgcagcg
     1741 tgatcctgtt tatcgattga cgcaccttca acttgcagga aaaatccttt ctcattttta
     1801 ctcaacaatt caatggtttt gtcggtcatc tgtgccaggg ttggtacact gtcattacgt
     1861 tgcggattag gcgtacaggt gactgcgggc ttatcgatat tgccgtggta cgttgctttc
     1921 ggccctagcc agcgcactgg catattgccg tcagcaaaca ggccaagcag gggtttttgc
     1981 tgattcgctt ccgtcaccga attcagtgag gcagcatcgt tcaccaactg ataaccacgc
     2041 gcctgtgcct gttcacgcag cgtttttccc tgccattcac cagcggttgc cgtttcagca
     2101 aaggtttttg cgccgccgcc aagcgtaacg tcggcacgag cgttaagcag ctgttcggta
     2161 atcgatcctt ttccgccttt ttccagagcg ttacccggac atttttcact ggtcgcgctc
     2221 ggaccgtagc atttgcgcga agtcacatgt gccaccagcg cagcgggcgt ggcatcctgc
     2281 aactctgcgg tagaaacgtt accggtcgcc agacctgcgg cttttgccat ttccagaatc
     2341 gttgggtgat ctttttcgtg aatatcgacg cccagcgcgc cgttataggt tttgacaccg
     2401 gttgaccagg cggttgctga tgcagccgag tcggtgacgt agtccggttt gccagttttt
     2461 ttattcagcg catagtgagt gtattgcccg gtaagcggta aggcatcgat acctttaaaa
     2521 aagccgcccg caccttcggc ataattacgt gcggcagtaa tttccgaatc ccccatccca
     2581 tcgccaatca gcaaaataat attttttgca ggtttatcgc taagagaatc acgcagagcg
     2641 gcggtctgat cactcgttaa gcggcgagca ccgccgggtg cagtaatatc gccctgagca
     2701 gcccggtttt ccagaacagg catttctggt gtccgggctt ttgtcacagg ggtaaacagt
     2761 aacggtaaga gtgccagtgc aatagtgctt tgtttcactt tattttctcc atgtacaaat
     2821 acattaaaaa ataaaaacaa agcgactata agtctcggcc gtgacaactt tatgacagct
     2881 gttgaaaaga ttaacttttt actgacgagg atgcttcaat aacttcttta cgtaatcgcg
     2941 cagcagctcc gtatcgtcgt caggaatgct ggcatcgggc tttacctcgt acagcgcccc
     3001 ctctacctga tcaatcaacc gctgttggtc attttgcgcc atattgcgaa gcattgcagt
     3061 gacgataatc tccaaagctt ctacctgcgc acacagttct ttcgactctt cttctttttg
     3121 ggcaagctta aataacaact cagcaatgag atttttcatg tctgtatttc cttatccaaa
     3181 gtatggagaa gttaacatta tgtttcacaa cttcatagtg tctggaagta ttatttttat
     3241 gacaaaggaa atactttgcg caaatatttt ttgtttgatt accagcgaaa cgtttctctt
     3301 atttaaataa ttacccacac aaaatatagg cttttaatta tcaaccttta
//
LOCUS       DAZPAC010000343         3333 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZPAC010000343 DAZPAC010000000
VERSION     DAZPAC010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.348"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            219..935
                     /gene="nanC"
                     /locus_tag="ACHUPM_004323"
     CDS             219..935
                     /gene="nanC"
                     /locus_tag="ACHUPM_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR0871927.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            955..2061
                     /gene="nanM"
                     /locus_tag="ACHUPM_004324"
     CDS             955..2061
                     /gene="nanM"
                     /locus_tag="ACHUPM_004324"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR0871928.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2126..3106
                     /locus_tag="ACHUPM_004325"
     CDS             2126..3106
                     /locus_tag="ACHUPM_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR0871929.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3216..3333
                     /locus_tag="ACHUPM_004326"
                     /pseudo
     CDS             3216..3333
                     /locus_tag="ACHUPM_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT          992 a          699 c          775 g          867 t
ORIGIN      
        1 tcagaagaga ttttatactc aggtatttaa tctgattctc tgtttattta aataatgtgg
       61 aaagagaatt ttcacaggag accttataca aaaatataaa ataagctacc cgttgccaaa
      121 gacactataa gcctggcaaa aaatattaca caacataaat gctaattgtt tatgcgggct
      181 ttgtattgct ttctgtatcc tacaaatgag tgaaatttat gaaaaaggct aaaatacttt
      241 ctggcgtatt attactgtgc ttttcgtcac cattaatttc tcaggctgcg acactggacg
      301 tacgtggtgg atatcgtagc ggaagccatg cctatgagac tcgactcaaa gtcagtgagg
      361 gatggcaaaa tggatggtgg gcaagcatgg aaagtaatac ctggaatacc attcatgata
      421 ataaaaagga aaatgccgca ctcaatgatg ttcaggttga agttaattac gcgattaaac
      481 ttgatgatca atggacggtg cgcccgggaa tgttaacgca ttttagcagc aacggcacac
      541 gctacggacc ctacgtaaaa ctgtcctggg acgcgacaaa agatcttaat tttggcattc
      601 gctatcgtta cgactggaaa gcttaccgac aacaagactt atccggtgat atgtctcgtg
      661 ataacgttca tcgttgggat ggatatgtca cttaccatat taatagtgat ttcaccttcg
      721 catggcaaac gacgctatac agcaaacaga atgattatcg ctatgcaaac cacaagaaat
      781 gggcgacgga aaatgccttt gttctacaat accatatgac gcccgatatt acgccataca
      841 tagaatatga ctaccttgac cgccagggtg tttacaacgg cagagataat ttatcggaaa
      901 acagttatcg cattggtgtg tcatttaaac tgtagtagac aggagacagt cacaatgaat
      961 aaaactataa tggcgcttgc tatcatgatg gcttcatttg ccgcaaacgc gtctgtatta
     1021 ccggaaactc ctgtgccatt taaaagtggt accggagcaa ttgataacga cactgtctac
     1081 attggtttag gtagcgcagg tacagcatgg tacaagctag atacacaggc caaagataaa
     1141 aaatggactg cgttagctgc attccctggt ggaccgagag atcaagcaac ctctgcattt
     1201 attgatggca atctatatgt gtttggcggc attggcaaaa acagcgaggg attgactcag
     1261 gtatttaatg acgtacacaa atacaacccc aaaaccaata gctgggttaa attgatgtcg
     1321 ctcgcgccga tgggcatggc gggtcatgtg acttttgtac acaacggcaa ggcttatgtt
     1381 actggcggtg ttaaccagaa tatcttcaat ggctattttg aagatctcaa cgaggctgga
     1441 aaagattcaa ccgctataga taaaatcaac gcccactatt ttgacaaaaa agcagaagat
     1501 tatttcttca ataagtttct gttgtctttt gatccctcaa cacagcaatg gagttacgct
     1561 ggcgaatcgc cctggtacgg aacggctggt gcggcggttg tgaataaagg tgataaaacc
     1621 tggcttatta atggcgaagc caaaccagga ttgcgaacgg atgccgtatt tgaacttgat
     1681 ttcaccggta ataatttaaa atggaataag cttgatcccg tctcatcacc agatggcgtc
     1741 gctggcggtt ttgcggggat aagcaatgat tctcttatat ttgccggagg ggccggattc
     1801 aaaggttcac gagaaaatta ccagaacggt aagaactatg cgcatgaagg cctgaaaaaa
     1861 tcatatagca ctgatattca tctttggcat aacgggaaat gggataaatc gggtgaatta
     1921 tcgcaaggtc gggcctacgg agtatcattg ccctggaata atagtttatt aattattggc
     1981 ggtgaaactg caggcggcaa agcggtgacg gattcagttt taatttctgt gaaggataat
     2041 aaagtcacag tacaaaacta acgcttcagg gccccggtaa aggggccctg ctatcaattt
     2101 gtcatttgta ttacgcgaaa attatatgaa cgcaataata tcgcccgatt attactatgt
     2161 tcttaccgtt gctggtcagt ctaatgccat ggcgtatggc gaaggactgc cattaccgga
     2221 cagggaagat gcgcctcatt ccagaattaa acaattagcg agatttgcgc atacgcatcc
     2281 cggaggcccg ccatgtcact ttaacgacat tattccactg actcactgcc cacacgatgt
     2341 tcaggatatg cagggttatc accatcctct ggcaacgaat catcaaacac agtacggcac
     2401 cgttggccag gcactgcata ttgcacggaa attactgccc ttcattccta ataatgcagg
     2461 gattcttatc gttccgtgtt gccgtggcgg atcggctttt accgcgggca gcgaagggac
     2521 atattcagaa cggcacggag ccagccatga tgcttgtcgt tggggaacgg atactccgct
     2581 gtaccaggat ttagtcagca gaacgcgagc cgcactggca aaaaatccgc agaacaaatt
     2641 cctcggcgta tgctggatgc aaggcgaatt tgacttaatg accagtgact acgcgtcaca
     2701 ccctcaacac tttaatcata tggttgaagc ctttcgtagg gatctaaaac aataccattc
     2761 tcagcttaat aatattactg acgcgccatg gttttgcggc gataccacct ggtactggaa
     2821 agaaaatttc ccgcattcgt atgaagctat ttatggcaat tatcaaaata atgttttagc
     2881 caatattatt ttcgtcgact tccagcaaca aggtgaaaga ggactgacga acgcgcctga
     2941 tgaagacccg gacgatttaa gcacgggata ttacggttca gcgtaccggt caccggagaa
     3001 ctggacgacg gcactgcgaa gcagtcattt cagcacggca gcccgtcggg ggattatttc
     3061 tgacagattt gtagaagcaa ttttgcagtt ttggcgcgaa aggtgagcgg ggagtttatt
     3121 catgggggat gtccttgttc gttctcagag tagacaacaa aaaacaaata agatcaaggt
     3181 aatatctctt cacttgcggg ctcgaacagc aggcaattgc caatataata atctggcaca
     3241 agggtgaaaa acgcgcaccg cataaaccgt tgttattgct tacagattag ccggatgcct
     3301 taatagacat ccgcgccttt tcgattatgg ctc
//
LOCUS       DAZPAC010000344         3294 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZPAC010000344 DAZPAC010000000
VERSION     DAZPAC010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.349"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(89..652)
                     /locus_tag="ACHUPM_004327"
     CDS             complement(89..652)
                     /locus_tag="ACHUPM_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR0871930.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(756..974)
                     /locus_tag="ACHUPM_004328"
     CDS             complement(756..974)
                     /locus_tag="ACHUPM_004328"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871931.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1133..1519)
                     /locus_tag="ACHUPM_004329"
     CDS             complement(1133..1519)
                     /locus_tag="ACHUPM_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR0871932.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1509..1880)
                     /locus_tag="ACHUPM_004330"
     CDS             complement(1509..1880)
                     /locus_tag="ACHUPM_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0871933.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1880..2137)
                     /locus_tag="ACHUPM_004331"
     CDS             complement(1880..2137)
                     /locus_tag="ACHUPM_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871934.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2134..>3294)
                     /locus_tag="ACHUPM_004332"
     CDS             complement(2134..>3294)
                     /locus_tag="ACHUPM_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0871935.1"
                     /translation="ITDTGRKTWVKSSLEHYVAALRRNAALRDAEKTLNEALQKLRDA
                     HTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEVVERVECRNQGLEKSRTLMT
                     GIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKVTEQGHGVLLFTMEMANIQI
                     GERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTGRNIWMVDQANLTIDEICAT
                     TKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISKGLKGLAKSGGFPLIALSQL
                     SRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVYNPDTQATGIAEINITKQRN
                     GSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYSNTRTDSSKMERFF"
BASE COUNT          766 a          915 c          728 g          885 t
ORIGIN      
        1 catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat tcaacttttt
       61 acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc accagttgct
      121 ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt tccagagtac
      181 ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt ttatcgaagt
      241 ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt
      301 tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga tcggtttcga
      361 gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag cgaacgttac
      421 gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt aacgcatcac
      481 cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga ttgaaattgt
      541 ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt gtcatgacag
      601 gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc atagacatgt
      661 cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca aaaccgtcga
      721 aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg gtcataaacc
      781 aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc tttcgaggtg
      841 ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc
      901 caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac cggcgagctt
      961 ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg acaataaaaa
     1021 acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc cgacgccaga
     1081 ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc acctacataa
     1141 ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac atgccatcaa
     1201 taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac ccatgtttcc
     1261 gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa tcgtgcaaat
     1321 cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca tcatcatcag
     1381 aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca gcagcgatcg
     1441 acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt tccataacct
     1501 gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac gcccagtcgc
     1561 ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg
     1621 agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga
     1681 cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat
     1741 ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc accaactttt
     1801 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1861 aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca aactttccaa
     1921 ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt tgtgcgcgat
     1981 gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg
     2041 ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca
     2101 attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca tcttactgct
     2161 gtcggtacgt gtattgctgt atctgcgcgg ctggggctgc cgcattgggg tggaaagaac
     2221 ctgtgcgctt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt
     2281 ccccagagaa ccgttacgtt gtttcgtgat attaatttct gctatgcctg tagcctgtgt
     2341 atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat ccgcctctat
     2401 ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg actccacacc
     2461 gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca ggcctttaag
     2521 cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg tttttatcag
     2581 cccgagataa tcaaccacca ccagcgccgt ttccggatgt ttaatcagat ggtgtttcgt
     2641 tgttgcgcat atctcatcaa tggtcaggtt cgcctggtcc accatccaga tattgcgccc
     2701 ggtcatccgc cccacccctt gtgagaaacg cgcccagtct tcatcttcaa agtgagccac
     2761 agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt caccaatctg
     2821 gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag tcaccttgtc
     2881 gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa tgaataccag
     2941 gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt
     3001 cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt ccggaagcac
     3061 atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt
     3121 ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac gtaatttctg
     3181 cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc
     3241 aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag taat
//
LOCUS       DAZPAC010000345         3285 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZPAC010000345 DAZPAC010000000
VERSION     DAZPAC010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.350"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(283..358)
                     /locus_tag="ACHUPM_004333"
     tRNA            complement(283..358)
                     /locus_tag="ACHUPM_004333"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:Asn,seq:gtt)
     gene            complement(459..1946)
                     /gene="yeeO"
                     /locus_tag="ACHUPM_004334"
     CDS             complement(459..1946)
                     /gene="yeeO"
                     /locus_tag="ACHUPM_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR0871936.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            2107..2182
                     /locus_tag="ACHUPM_004335"
     tRNA            2107..2182
                     /locus_tag="ACHUPM_004335"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2140..2142,aa:Asn,seq:gtt)
     gene            complement(2220..3170)
                     /gene="cbl"
                     /locus_tag="ACHUPM_004336"
     CDS             complement(2220..3170)
                     /gene="cbl"
                     /locus_tag="ACHUPM_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR0871937.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          963 a          857 c          692 g          773 t
ORIGIN      
        1 aattacgcat agtttcccga taaagacgcg atagcgacat cccgcataag gcatttttct
       61 ctttatcttt gtacggtact tcatggaaca gagtttttga ccttgcgaat cgtgatgtct
      121 gttggggagg ggcaatttgc tcactgaagc gtgagactcg attaagcgca cgaaacacag
      181 aaatcaaaaa acccggtcac ttttttacaa ggtaaccggg taaaaataat ttttattttt
      241 taactgtttt gagactcata gagatgtctc aaaactaaaa tttggctcct ctgactggac
      301 tcgaaccagt gacatacgga ttaacagtcc gccgttctac cgactgaact acagaggaat
      361 cgtgagaacg aggcgaatat tagcgatgcc cacccacaat gtcaaagcct gttttttaaa
      421 tttgaaatcg tttgctgaaa taatctgcat tttgtcgttt attccgacac aactggcttt
      481 ttttcacact tttgcggttc ggatcgaggg tatttccaca gccaacgtcc agtaaccatt
      541 cgccagtaaa acagcacggc ccgcacagcc cagtcggcaa acattcccat ccagacacca
      601 accacacccc agccaagcat gatgcccagc acataaccga cgacaacccg gcaaccccac
      661 atgctcaaca tagaaaccca catggcgtaa cgggcatcgc gagcaccttt aaatccagcg
      721 ggtagcaccc atgaggcgga ccaaataggc ataaataaag catttagcca aatcagaatc
      781 acaacgacat gtttaacctg tggatcctgg gtgtaaaacg atgccataac cccggcaaag
      841 ggagccgtta gccaggcgat ggccgttaat ccaagagtgg aaagccagaa cacatgccgc
      901 aactgaatct ctgcttgcgc tatctgccct acccccaacc ttcggcctgt aatgatcgta
      961 gaagcagagc cgagcgcact tccgggtaag ttgataagag ccgcaattga aaacgcgata
     1021 aaatttccgg caataacact ggtccccatc ccggcaacga acatttgggt taataaccga
     1081 ccactggtaa ataacactga ttcgacactc gcgggaatac caatccccat gacttcccag
     1141 ataatgctaa aattcagcgg tttaaaatag ctctttaacg aaatccttag cgcaggatta
     1201 aaaccaatcg ccagcaccca caaaattgca actgcgccaa tataacgaga aatggttaaa
     1261 cccagccctg ccccgacaaa tcccagtccc ggccaggaga aaaggccgta aatcaatatg
     1321 ccgctaataa taatattaag aatattcagg ctaccgttaa tcaatagcgg tattttcgta
     1381 ttccctgcac cacgaagtgc cccgctacca ataagagtga tggcagctgc tggataactg
     1441 agtaccgtca gctccagata agtcaacgcc agtgctttaa cttctgtcgt ggcatcaccc
     1501 gcgacgaaat caataatttg ttcgccaaaa tgatgaataa gcgttgccaa cagtacggca
     1561 aacaacgtca tgatcaccaa tgactgccgc gtcgccaccc tcgctcgtcg tcgatcccgc
     1621 ttaccgagac taaatgccac aacgacagta gtaccaagat cgatagcagc aaaaaaagcc
     1681 ataatgacca tattgaagct gtccgccaat cccacgccgg ccatcgcatc ttttcccagc
     1741 cagctgacca gaaaagtgct cagaaccccc atcaacagga cacaggcatt ctccatgaag
     1801 ataggaacag caagcggggt tatctcgcgc cagaacaaca ctttgtagct cttgcgttta
     1861 gcgtgccagc gagtgccgtg aacaacctgg cgtaaagcag aggagatatt caaagccgac
     1921 cttaattgca gaaagtgaaa ccacatttca aataatgagg gagaatcagc aaagctgcaa
     1981 agattttcgc caacaaattg tctgcaaatg caacaaactg ttgatagaaa cggcaaacag
     2041 ttggggaatt taaaaatcgg gtttgacaaa agatttttcg ccgttaagat gtgcctcaac
     2101 agcgattcct ctgtagttca gtcggtagaa cggcggactg ttaatccgta tgtcactggt
     2161 tcgagtccag tcagaggagc caaatttctg aaaagcccgc ttttatagcg ggctttttgc
     2221 tatatctgat aatcaatttc ctcttcactg ttttccatca cctgccgctt gatatcctca
     2281 actgacagtc ctgcattaca aagttccaga aagcgccaga catagttacg ctgaagttgt
     2341 cctcgcttca gtcccaacca gacagtatta gcatcgaaaa gatgccgcgt atccagacgg
     2401 attaaattct cttcctcttg ttcgccgctg gattgttcgg caactaatcc gatcccaagc
     2461 ccaagagcaa cataggtttt aatgacatca gaatcctgcg cacttaatac aatatctgcc
     2521 agcaaacctt tgcgggcaaa tgcgtcatca atacgtgagc gccccgtaat cccctgtcgg
     2581 taagtgatta acggccactt cgctattgat tccagcgtca atggtgtaat ttgcgttaag
     2641 ggatgatcga gtggaacaag caaactatgg tgccaacgaa accacgggaa ggcgacgagc
     2701 tgcgggtcat tactcaaacg ctcgctggcg ataccaatat cagcttcgcc attttgcaac
     2761 aacgtcgcaa tttcctgtgg cgtcccctgg attagctcga gccgaacctc cgggaaaagt
     2821 tcgcgaaaag ctttaatgac ctctggcaag ctataacgtg cctgagtatg cgtcgttgca
     2881 atagtgagaa cgccagacgt atcgttggta aacaggtctg caagccgacg aacattactg
     2941 gcttcattca gaatacgttc tgcaatgacc agtaatgctt tgcccggttc agtcatgccc
     3001 agcagtcgct tacctcgtcg aacaaatatt tcgatgccaa gttcatcctc cagttcccga
     3061 atatgacggc tgacgccgga ctgtgaggta aaaagcatat tcgcaacctc tgtcaggttg
     3121 taatcctgac gtgcagcctc gcggattatc tttagttgtt ggaaattcac ggtaaactcc
     3181 gggcagttca gatttcccgt tattgttaaa gtctaatgcc cggcataaca aataataaaa
     3241 acccgcatct tattccatcc cgatataacg cttagctcac caatt
//
LOCUS       DAZPAC010000346         3285 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZPAC010000346 DAZPAC010000000
VERSION     DAZPAC010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.351"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..229)
                     /locus_tag="ACHUPM_004337"
     CDS             complement(<1..229)
                     /locus_tag="ACHUPM_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871938.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKK"
     gene            403..1116
                     /locus_tag="ACHUPM_004338"
     CDS             403..1116
                     /locus_tag="ACHUPM_004338"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0871939.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            1148..1906
                     /locus_tag="ACHUPM_004339"
     CDS             1148..1906
                     /locus_tag="ACHUPM_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871940.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1952..>3285
                     /locus_tag="ACHUPM_004340"
     CDS             1952..>3285
                     /locus_tag="ACHUPM_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR0871941.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          750 a          790 c          854 g          891 t
ORIGIN      
        1 atttttttgc tgttttcacc tgatttcgtt caattgtttt atgtaaggat tttcccatca
       61 acggtaattt tgtcactgca cgaccgacat ttccggcctg ttttcctaaa tgaactcttt
      121 cggcagcacc atatttttcg acaacggtaa taagatgttc acacaccgtt gacttgcctg
      181 aaccatcgct accaataacg gcaattaatg gaggtgttat tgactgcatg aatatatcct
      241 ttaatttaaa catccattaa aaatatttat ttggttaata tgtttttatg aaagcgtaat
      301 tcaggtcaat gtcacaatta accattgtca caataaggtt gaacggatat tctgagcacg
      361 gaccacgtaa tattcaatac attatttact gccgggtttt tcgtgaatca acgtaatatg
      421 tcaataatta attccacgcc agtgcgtgtt attgccattg taggatgtga tggttcaggt
      481 aaatcgcccc tcacggcaag cctggtaaat gaactggcag caagaatgcc aacagaacac
      541 atttatctcg ggcaatcgtc cgggctaatt ggcgaatgga tttcacagct ccctgttatt
      601 ggcgcacctt ttgggcgtta tctgcgaagt aaagcggcac atgtgcacga aaaaccctca
      661 acaccgcctg gcaatattac tgctctggtt atctatctgc tttcctgctg gcgggcgtac
      721 aagtttcgca aaatgttgtg taaaagccag caaggctttc tgctcatcac cgaccgctac
      781 ccgcaggttg aagtgccggg ttttcgcttt gatggcccgc aattggcaaa aaccacgggc
      841 ggtaacggtt ggataaaaat gttaaggcag cgcgagctga agctgtacca atggatggca
      901 tcttatttgc ccgtattgtt gattcgtctt ggcattgatg aacaaaccgc gtttgcgcgt
      961 aaacctgacc accaactggc agcgttacag gaaaaaatcg ccgttacgcc gcaactgact
     1021 ttcaacggcg ccaggatcct tgaactggat ggtcggcaac cagccgatga aatcctgcaa
     1081 gcgtcactac gtgcaattca cgccgcgctt tcctgatcac caaaatggat aggggatgat
     1141 ttaacgaatg gatgcactac aaacacaaac tgttaatagt acaaccgcgc cgcagcccag
     1201 ctacattccg gggctaattg cggtggtcgg gtgcgatggc accggtaaat ccacgctgac
     1261 caccgatctg gtgaaatcat tgcaacagca ctggcaaacc gagcggcgct atctggggct
     1321 gctctcgggc gaagacggcg acaaaatcaa acgattaccg ttggttggcg tctggctgga
     1381 acggcgactg gcggccaaat cctcgaaaac ccaaagcatg aaaaccaaat ctccggcgct
     1441 atgggcggcg gtgattatgt actgcttatc gctgcgaaga atggcgaatc tacgcaaggt
     1501 tcagcgactg gcgcaaagtg gcgttctggt ggtcagcgat cgcttcccgc aggctgaaat
     1561 ttcgggcttt tattacgacg gaccggggat tggcgtcgaa cgtgcgaccg ggaaaatcag
     1621 catgtttctg gcgcagcgcg aacggcgttt ataccaacaa atggcgcaat atcgcccgga
     1681 attaattatt cgcctgggca ttgatattga gactgccatc tcccgcaagc ctgaccatga
     1741 ctatgccgag ctgcaggaca aaatcggtgt catgtcgaag attggctata acggcacaaa
     1801 aattcttgag attgattcca gagcgcccta cagcgaagtg ctggaacagg cacagaaggc
     1861 ggtttctctg gttgccatcg tttctgaccg ccgaagttta acttaaggct ggcgggatac
     1921 tttttactat caacgataag gtcaggctga attggcgggt tttaacatca aacattggtt
     1981 tgcagatggc gcgtttcgca ccattattcg caatagcgcc tggttaggct ccagtaatgt
     2041 cgtgagcgcc ttgctggggc tgttggcgct ctcgtgtgcc ggtaaaggga tgacgcccgc
     2101 catgtttggc gtactggtga ttgtgcaatc gtacgccaag tcgatcagcg attttattaa
     2161 gtttcagaca tggcagctgg tggttcagta cggaacgcca gccttgacca acaataatcc
     2221 gcagcaattt cgcaatgtcg tctcattttc cttctcgctg gatatcgtca gcggcgcggt
     2281 ggcgattgtc ggcggcattg ccttactgcc attcctttcc cattcattag gtctggatga
     2341 ccaaagcttt tggctggcgg cgctgtattg cacgctcatt ccttcaatgg cttcctccac
     2401 gccgaccggc attctgcgtg cggtagatcg cttcgattta attgctgtac agcaggcgac
     2461 gaaacctttt ctgcgcgcag cggggagcgt cgtagcctgg tattttgact ttggttttgc
     2521 gggttttgtt attgcctggt acgtgtcgaa tctggttggc ggcaccatgt actggtggtt
     2581 tgcagcgcgc gaattacgcc gcagaaatat ccataatgcc ttcaaattga atctgtttga
     2641 gtctgcccga cacatcaaag gcgcgtggag ttttgtctgg tcaaccaaca ttgcccactc
     2701 catctggtcg gcgcgtaact cgtgcagcac agtgctggtg gggatcgtgt taggacccgc
     2761 tgccgccggg ttatttaaaa tcgccatgac attctttgac gccgccggaa cgccagcggg
     2821 tttgctgggt aaaagttttt acccagaggt gatgcgttta gatccgcgca ccaccagacc
     2881 gtggttgtta ggtgtgaagt ccggtttact ggcgggcgga atcggtattc tggtggcgct
     2941 cgcggtgttg atcgccggca agccgctcat ttcgctggtg tttggcgtta agtatctcga
     3001 agcgtatgac ctgattcagg tgatgctggg cgcaattgtg atttcgatgc tgggtttccc
     3061 gcaggagtca ttgctattga tggcgggaaa acagcgcgct tttctcgtgg cgcaaaccat
     3121 cgcctcaatc ggctacatcg tattgctgtt catgttctgt catctgtttg gtgtgctggg
     3181 agcagcgttt gcttacttcg gtgggcaatg tctggatgtg gtgctctcgt taattccaac
     3241 tttaaaggca ttttttcagc gtcattcctt gctttataac gcagc
//
LOCUS       DAZPAC010000347         3236 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZPAC010000347 DAZPAC010000000
VERSION     DAZPAC010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.354"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..401
                     /locus_tag="ACHUPM_004341"
     CDS             <1..401
                     /locus_tag="ACHUPM_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0871942.1"
                     /translation="IKEDDFLSLDELNDFLSMNESDLEVSSKKTSKQDREITDVSVDS
                     DHKNTQCPATDDELDPFDDEEDEREKAIKRAEKKKRFELARQKNKVSGLDPFDDSEDD
                     YYLSPKKKQTQDDKKQKIKPKMYFAGGGIK"
     gene            401..691
                     /locus_tag="ACHUPM_004342"
     CDS             401..691
                     /locus_tag="ACHUPM_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871943.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            1012..1302
                     /locus_tag="ACHUPM_004343"
     CDS             1012..1302
                     /locus_tag="ACHUPM_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871944.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            1302..1721
                     /locus_tag="ACHUPM_004344"
     CDS             1302..1721
                     /locus_tag="ACHUPM_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871945.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            1889..2212
                     /locus_tag="ACHUPM_004345"
     CDS             1889..2212
                     /locus_tag="ACHUPM_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871946.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            2642..>3236
                     /locus_tag="ACHUPM_004346"
     CDS             2642..>3236
                     /locus_tag="ACHUPM_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871947.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1076 a          576 c          674 g          910 t
ORIGIN      
        1 atattaaaga agatgacttt ctttctttgg atgaattaaa tgattttctc tctatgaatg
       61 aatctgacct tgaagtaagc tctaaaaaga cttctaaaca agacagagaa atcactgatg
      121 tgagtgttga tagtgaccat aaaaacacac agtgccccgc cactgatgat gagttggacc
      181 ccttcgatga tgaagaggat gaacgtgaaa aagccattaa gagggcagag aagaaaaaga
      241 gattcgagct tgcaagacaa aagaacaaag tatcgggttt agacccattc gatgacagtg
      301 aagatgatta ttatctttca ccgaaaaaga aacaaactca ggatgacaag aagcaaaaaa
      361 ttaaaccgaa gatgtatttt gccggtggag gaattaagta atgaaagaat atcacggtga
      421 aaagagatac aaagattacc tgttaaagcg ttacagcatt tctcgtgaag gtcatcttat
      481 gaaggatact cacggggaag tttaccggat tagaccaaag aaagaaggga ggggttattt
      541 cttttacgat ggtgtgacag atttaaagat tgacgctttg aggtttgcag tgctgtatca
      601 ttttgatatc tggaattcaa tccaccagct acgattaaaa gacggtgacc ccagcaactt
      661 aaaggccacc aatattatca caaaacgtta gtttgtttta ccatcatcaa tcgtaattgt
      721 gatttcatat ttgaaaagct taatgctgaa attgaatcgt gtacttctgg cgataatcat
      781 ttttaaaagc tgatacacaa gaagcaccag aaacacaaag caacctaagc ttgcgaatct
      841 gtgagattca gcaattgttt caagaaagcc cattaattcc ccttatttat caaagggaat
      901 gacctgtatc agccccctac aaccatatta acatgtaaat gagttgttga caatatgtta
      961 taaatatgtt aaagttagtt ataagtcaaa gattttaaga aggaaaattt aatgagacac
     1021 acagttacac gccttttgcc agaggcatat tccagactgg cagcaatcaa agacaaatac
     1081 ccagccatga gccacacagc acttgttggt gagattttga aggaacacat agcccttaca
     1141 ccgtttgacg ctatcaaggg cgttgcagag ggttctaagg ccatcagaat cacaacgggc
     1201 caactggaac tactcaaagc acagtccgca gtggcaggtt gctcaatgat tcaggtgttg
     1261 agtgccaagg ttcatcaact ggcggataag ctgggggttt aatgctatgc acatttgatt
     1321 atcatgcgtt ttatcgccat agaggtcacc ttgtccgtga gaataacgga acatggaaag
     1381 atttaactcg taatgctgaa cccttcacac accagcaatt aaaatcccgc ttctccagat
     1441 ggaagaatgc accagcagat actggtgatt ttgagattat ccataccttc gattatgcct
     1501 cagaagatga ccttcacgat gcaattaagc tgattgaaga cttcaatacc tcagaagatg
     1561 tggatggtga caaatataaa tcccgtggta accgctataa ccgtgatgat atcactcagg
     1621 ccataaatgc tggtcaatct cagcgataca ttacagaaaa atatggtatt tcacggagac
     1681 agtatttcta cattaagcca ttgattaata aagacaatta aaaataacca tctaagaaac
     1741 ggtagaatgc actttacagg cagagttgca ccttaatttg ataaattacc ttaccagcca
     1801 gtgcaactcc ctctgtaacc cgcatggtta ctggcctcag aatggctatt ttaagaggtc
     1861 tgaggtgtgt ttcataggag atttaaaaat gaataattac ccaatgcaaa ttatggttga
     1921 taacgacact gctatgatgg tgcaaagctt cattgatgca ggtatcgcaa ttaattttga
     1981 taaattacta aagctgatgg ctgataatgt agaaaacatc tctgacttta ttcagtcggt
     2041 ggagtttaac gagcctcgaa tgatgctacc aattacagac agcaacatga aaagattggt
     2101 tatcgagcaa acgaacatat acagtatttc cccagagcaa tttttgaaag gtgctgtgac
     2161 aatcctgtac agtgataata ttctggtgac ggattctgtg agggttcact aatgggttta
     2221 ataattgctt ggacgttgct tggcttattt gttggttgta ttattaacaa tgcggtaaat
     2281 aagaattgat gttatcaacc tgttaaaaag ctgttgacaa ataaccttaa gcatggcata
     2341 attttaatta tgggagagca attctctctt atttttcttt caataatccg atggatggat
     2401 attctgagtt ggaaaggtgc aatgcttttc gtcatggcct ttcaggttag tctgagaggc
     2461 tatctcaaaa agtactcttg ttcattcatt attgaatcct ttgcccttca cagaaatgtt
     2521 tagggctttt tcagttttta aaagcacact tggcaggttc ttgttcagtc agactcttca
     2581 cctctgattg ctgagtgtgc ttctataaat tgaaataaca aataaactaa ggggtgaacg
     2641 aatgattaga aatgaacatg aaatgcttat cgaacacaga ccaagtgcac ttgatattca
     2701 cgaattaact ttgcgtgaat cttttgatag agcttggcgg gaatcacacg aagtaaataa
     2761 aggtccatat attccaccag aaccactcga gattccaaga gttgaaattg atttttctat
     2821 gaacgaacgg tgtgaacttg atttgaagtt gaagctacaa cgccgatatt tccaagctat
     2881 taaagactct caagaagcaa tgagaagtgt ttacgagaaa attcagtcca agcttgaaga
     2941 cgaaattgac gaaatggtta tttcgatgga gcttcaaacc aattcttatg cttatttctc
     3001 tcgactagac cgtgacggtt gggggtatga ccctgtagag attggtaaag tgattgcaga
     3061 tttaccagaa ggtcaaagag ttgttcgtac agaaagagct gtcagagcac cacatcgact
     3121 gtctctaatt gttgctgata aatcacaagc tgagcttgaa gaaattgcca aagagaaact
     3181 gatgaaagca agagaaaatg agattaattt gctgaaatca agactggcta agctga
//
LOCUS       DAZPAC010000348         3216 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZPAC010000348 DAZPAC010000000
VERSION     DAZPAC010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.355"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(147..1359)
                     /locus_tag="ACHUPM_004347"
                     /pseudo
     CDS             complement(147..1359)
                     /locus_tag="ACHUPM_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1631..1725)
                     /locus_tag="ACHUPM_004348"
     tRNA            complement(1631..1725)
                     /locus_tag="ACHUPM_004348"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1689..1691),aa:Sec,seq:tca)
     gene            2018..>3216
                     /locus_tag="ACHUPM_004349"
     CDS             2018..>3216
                     /locus_tag="ACHUPM_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFR0871948.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
BASE COUNT          809 a          719 c          751 g          937 t
ORIGIN      
        1 aaaaatgtca atttgttttt aattatgttt ttgtaataag tttgaagacg caaaaaaaat
       61 aatataataa caaaaatcct ccagccttgt aggccagagg ataaaatact taattgagga
      121 aaatatcagg gaaaagtatt tctgacttaa ctttcccggc tggagagtga atcaatgtaa
      181 tcagcgtacc attgcatcat ttcacggcgg ccatcgagat acagggcatg attataggta
      241 cccctgatgg aatttttatc cacatgagct aactgcattt caatccaggc ggaattaaag
      301 ccttgttcat gcagaattgt actcatggtg tgtctgaaac cgtgccctgt gagtcttccc
      361 ttatatccaa gtaattcaat tacttggtta atgctttcct tactaattgg tttgcgggga
      421 tcatttctac cgataaagac taggggatga tgttttgaga ttggttcaag ctgcttaaat
      481 agcataatga cctgctcaga gagcggcaca atatgaggtc gcttcatctt cattacttct
      541 gccgggatct cccacaattt tgtctcaaaa tcgatatctt cccagcgtgc aaaacgcagt
      601 tcttgtgttc gtacgccggt taacataatg atctgagtag ctgtcttagt gattatactt
      661 ccggtataac ccgccagatc gttgaggaag tggggaagtt cattagcagt aagaaaggga
      721 aaatgtactt ttttcggtgt agctaaagca ctggcaagat cgggggctgg gttatagtct
      781 gcccggccag taacaattgc atagcggaac acctcaccac agcgctggcg gaccttgcgc
      841 attttctcca gagctccacg tttttccatt ttacgcagtg cttccagtaa ctccattggt
      901 ttgatttcgg caatagggcg cttgccaatg tagggaaaaa tgtctttttc aaaggtatca
      961 atgatttcat cacgatagcg taatgaccac cgatcagctt ttgactggtg ccattcgcga
     1021 gcaatagcct cgaacgtatt tttcagactc atttgctgcg cgattttttc ttcctttttt
     1081 gcctcccctg gatcgcctcc agcagcgagg agtttcctgg cttcactgcg tttctcgcga
     1141 gcatcggcta gcgttacatc ggggtatacg ccaatagaaa gcatcttttc tttaccagca
     1201 aagcggtact tcatgcgcca gtatttagaa ccattagtgt taatcagtag atacataccg
     1261 ccgccatcag caagcttata gggtttgtct ttgggttttg cggttttcac tttgatatcg
     1321 gttaatgcca tgacatcctc cggcttttga tgaattcatg ttcctgaggg ggatttctgc
     1381 cagtaactaa cctgataccc ccaatacacc ccttgtataa caaagatatt gttaaaagcc
     1441 gttgttatgt atcacatttt tcctattatg ggggtgcttg tatttcaata ggaaataaca
     1501 ccccctaaag tacccccaaa agaaacttga tatggggtga agtaggttga cctcaggata
     1561 caaataaata ctgatataca agggattaga agagatttat tgacattggg agagttcaga
     1621 agaggtaaac tggcggaaga tcacaggagt cgaacctgcc cgggaccgct ggcggcccca
     1681 actggatttg aagtccagcc gcctcaccgg agacgacgat cttccgcgcc tcgattgcta
     1741 catggaggcg gggcgcatta tagctacttc cttgagtttc tacatccccc agatcagttt
     1801 tttccgccta atacacgcag cgtattatca cgaaagcatt ataactcata taaaacccat
     1861 attttacaat gatatatgag cggaattaac gctaccacga tcacataaaa caagaatcat
     1921 aaattaataa ccagatatcg gaatattcgc tctcacaggg atggtttaca aaatgcgtta
     1981 tcggtctcag cacccctata gcatcaagga aaagcagatg aagagtgaag tgttgtccgt
     2041 taaagagaaa attggttatg gcatgggaga cgccgccagc cacattattt tcgataacgt
     2101 aatgttatat atgatgttct tttataccga tatttttggc attcctgccg gatttgtcgg
     2161 aaccatgttt ttggtcgctc gtgcactgga tgcgatttcc gatccttgca tggggttgtt
     2221 ggccgatcga acgcgctctc gctggggtaa atttcgtccg tgggtactgt ttggcgcact
     2281 gccattcggg atcgtctgtg tactggccta tagcacgcca gatctcagta tgaacggcaa
     2341 aatgatctat gcagcaatta cttacaccct acttacctta ctttataccg tcgtcaatat
     2401 cccttactgc gcattgggtg gtgtaatcac caatgacccg actcagcgta tctcgctgca
     2461 atcctggcgt tttgtgctgg cgaccgcggg aggcatgctt tctactgttc tgatgatgcc
     2521 actggttaat ttaattggcg gtgataataa accactcggt ttccagggcg gtatcgcggt
     2581 cctttccgtg gtggcattca tgatgctggc attttgtttc ttcaccacta aagaacgcgt
     2641 tgaagcacca cctacaacaa cgtctatgcg ggaagattta cgtgatatct ggcaaaacga
     2701 ccagtggcgg attgtcggtt tactaaccat tttcaatatc ctggcggtgt gcgtacgcgg
     2761 tggggcgatg atgtattacg tcacatggat tttgggcacg ccggaagtgt ttgtcgcttt
     2821 tctcaccact tattgcgtgg gtaacctgat tggttccgca ctggcaaaac ctctgaccga
     2881 ctggaaatgt aaagtcacta tcttctggtg gacgaacgcc ctgctggcag tgattagcct
     2941 cgcgatgttc tttgttccca tgcaggccag catcactatg tttgtcttca tcttcgtgat
     3001 tggtgtgttg catcaactgg tgacacctat ccagtgggta atgatgtccg ataccgtcga
     3061 ctacggcgag tggtgcaatg gtaaacgcct gaccgggatc agttttgctg gcacgctgtt
     3121 tgtgctcaaa ctggggttgg ccttcggcgg cgctcttatc ggctggatgc tggcttatgg
     3181 cggatatgat gcggcagaaa aagcgcagaa cagcgc
//
LOCUS       DAZPAC010000349         3188 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZPAC010000349 DAZPAC010000000
VERSION     DAZPAC010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.356"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            452..865
                     /locus_tag="ACHUPM_004350"
     CDS             452..865
                     /locus_tag="ACHUPM_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871949.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGALHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            867..1646
                     /locus_tag="ACHUPM_004351"
     CDS             867..1646
                     /locus_tag="ACHUPM_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR0871950.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            1826..2470
                     /locus_tag="ACHUPM_004352"
     CDS             1826..2470
                     /locus_tag="ACHUPM_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFR0871951.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            2557..2865
                     /locus_tag="ACHUPM_004353"
     CDS             2557..2865
                     /locus_tag="ACHUPM_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFR0871952.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
BASE COUNT          779 a          736 c          807 g          866 t
ORIGIN      
        1 atctgatatg attttatgtg tgttttttat tatcatatca tttgcgtctt ttcttccgcg
       61 cagatccggt ttccggcggt tcaggatctt cctctccccc cacacaacgc cacctcctgt
      121 cagcacaaca tgtggtatct cattcagctg cttatgacac tatatgttgt gtcatctccc
      181 tgacctgtga tgcgccgcgc aggggcggaa aacagcgata tgatgatttt ctcagcgtgg
      241 tacacttccg gaaagttgtg atattccgga aagtcggatc tgacggaaac ggctctccgg
      301 taatttaacg gcgtggttat atggatgctt gttatcatgg tgatgatgat aacggcatga
      361 tgttatctga cggcgtgacg gtaagggcag tgatgatgga tgacgttatc gcatgaccgt
      421 ccctgcccgg aaaagaaaaa aggagtcacc catgtttttt attgagaatg aaggtcaggc
      481 tgtcgccgga acggattact ggcagtctgt acaggcgcag gccggatatg tctacctcag
      541 ctggaatgcc ggcgcagcca ggctgcttgt cccggatgcg gcaaaacatt tactcaggga
      601 gatgcggggg gctgagtacg tcatcatcag taagggagca ctgcatggcc gcgatgcgct
      661 ggaactggta tttgaagacg gcagcgatgc gccgtttgtg atccacatgc tgagtgagca
      721 gtgcgatcgc ctgctccccg aaaacaacca gggagggggt tttgttgtca ccgtctggac
      781 gcgtggcggt aaccagctcc gttatccggg aaagtaccgt gttgtggaaa acctgcccga
      841 cgtttccccg tggagtgaac actgatatgc agcacctgcc ggcaccgatc caccatgccc
      901 gggatgccgc tcagcttcct gttgccatcg attatccggc agcgctggca ctccgccaga
      961 tgtcgatggt tcatgatgaa ctgcccaaat acctgctggc ccctgaagtg agcgccctgc
     1021 tccattacgt cccggatctg cgccgcaaga tgctgctggc cacactgtgg aacaccggtg
     1081 cgcgcattaa tgaagcactg gcgctgacgc ggggggattt ttcgctcacg cccccgtatc
     1141 cgtttgtgca gcttgcgacc ctgaaacaac ggacggaaaa agccgccagg acggcaggaa
     1201 gaatgcccgc cggtcagcag actcaccggc tggttccgct ctccgacgcc tggtacgtca
     1261 gccagctgca gacgatggtg gccacactga aaatccccat ggaacggcgt aataaacgaa
     1321 caggcaggac agagaaagcg cggatctggg aagtgacgga cagaacggtc aggacctgga
     1381 ttggggaggc ggttgccgcc gctgccgctg acggtgtgac gttctctgtc ccggtcacgc
     1441 cacatacgtt ccgccattcc tatgcgatgc atatgctgta tgccggtata ccgctgaagg
     1501 tcctgcagag cctgatgggg cataagtcca tcagctcaac ggaggtctac acgaaggtgt
     1561 ttgcactgga tgtggctgca cggcaccggg tgcagttttc gatgcctgag tccgatgcgg
     1621 tgtctatgct caaaagaatt ccataagctc tgactttttt aaatcccttt cggatatctc
     1681 atggatatat tatcggtatt tcatattctg ttgacatgca ggaccggtat agagtaaaaa
     1741 aacatctatt gagtacctct tggatatccg aaaggtgtct tatggggtct ttatgggtat
     1801 tacatgtgga ttctggagat acactatgcc tgttattgct aatacgcatc ccaaaggtgg
     1861 tgtgggtaag acaacttcat ccgtaaacat tgttggtgaa atgaagtctg atacagttga
     1921 tctggatact cataccgggc tttctatcat tctggggctg agacctgaag gaaaagaaat
     1981 ttccgtgaaa gtacccaaaa cagtggatga gttaatcgaa attatgactc cctacaagaa
     2041 cagcgataag acgttactta ttgactgtgg gggatttgac tcagatctta cccgtactgc
     2101 gattgccttt gcagactgcg ttattgtccc ttcaaaagac tccctgactg aacgtattgg
     2161 tttgatgcat tttgatggag tactggacga aatcagctct atcatgggga ccgatataac
     2221 tgctcacctg tatctctgta aagttaatcc taacaagaag aaattcccta agcttgatgc
     2281 aatcctgcca tcgttcaaac atctgaagct gatgaagagc cgcatttctg ctcgtgcaga
     2341 atttgaggat gtcattgaga ctgggatggg aatcactgag tctgttcatg ggcgctattc
     2401 ggcaggaggt aaggaagtta ttgccctgat cgaagaaatc aatcatctga ttgaaaataa
     2461 caaacagtaa ggtgtgtcta ttgggtatcc atatagtatc ctttgggctt ttaatggata
     2521 tcttttaagt atctaacaaa catcctgggt tatctcatga gcaaagcgaa atttagtgca
     2581 caggctgaaa aactgaaaaa cctggtggat tctgccagat tacaaacttc ttcttctgcc
     2641 gtaacagcaa cagccccgtc tcagccaaat gaaggtgcag aggaagtttc aggtcgtccc
     2701 gtggctgcca ggacgaaggc taagaatctt aaggccatac cgctgtcgta ttttgaagct
     2761 catgcggaac ttaaagcgac cagtaaaaca tctctggatt ttagctcata tattattgag
     2821 gctataagag agaagcttga acgtgatggt gcaataggtc aataaactat cggatacctt
     2881 aagggtatct gatgtgtgtc ttttaaatgc gttatgggga ttcttcacgt acctaaagag
     2941 tccccattag acatctttct gtttatggtg acatgattcg tcaccgcttt cctttcctct
     3001 cagtttcacc atttcattcc ttttccctta acctgaacca tgatgtccac gatgttttta
     3061 tattgcattt atgtgtcaca tataaccttt ataatgtttt tgtatctatg atttcctctg
     3121 gtcttaaagt tgtgtgtaca atgtacatac aatttatcgt gtttttatgt tgcatttgtc
     3181 ctttcaga
//
LOCUS       DAZPAC010000350         3183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZPAC010000350 DAZPAC010000000
VERSION     DAZPAC010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.357"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHUPM_004354"
     CDS             complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHUPM_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR0871953.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHUPM_004355"
     CDS             complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHUPM_004355"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR0871954.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1715..1984)
                     /locus_tag="ACHUPM_004356"
     CDS             complement(1715..1984)
                     /locus_tag="ACHUPM_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR0871955.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2054..2638
                     /gene="mobA"
                     /locus_tag="ACHUPM_004357"
     CDS             2054..2638
                     /gene="mobA"
                     /locus_tag="ACHUPM_004357"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR0871956.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2620..3147
                     /gene="mobB"
                     /locus_tag="ACHUPM_004358"
     CDS             2620..3147
                     /gene="mobB"
                     /locus_tag="ACHUPM_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR0871957.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          832 a          771 c          769 g          811 t
ORIGIN      
        1 acattgaatt attttttctc ggacagatat ttcactgtat cagcatactg ctgaacaaaa
       61 acatccatat tgctggtatc cataccctgc ggattcagct gatatttacc gttaacaaac
      121 atcgccggaa cgccacgcaa ttgcacgtca gctgcagctt tttcctgctg agcgaccaga
      181 gatttgacca cgaagctgtt ccacgccgcg tcgtactctt cacctttaat acctgcgttg
      241 ataaatacat cgcggatatc agatgctgaa cgaatggtct gggttttctg tacgccttca
      301 aacagcggaa cagtgacttt gtcttccacg cccagcgcca tcgccacagc ccatgcctga
      361 gtcagatctt tgcccaggtc accacccatg aagttgacgt ggtatttagt catcttcacg
      421 ccttccggca gttttttctt cacattatca gaaatatgca gaacttcttc aaactgatag
      481 cagtgcggac agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc taccggtttt
      541 tccagggtag tgtactgttt accatcttca tactgcgccg ccgatgcgct aaacgctaaa
      601 actaaaccag ccagcgccag ccaaatcttt ttcatgatct actctctccg attaatacat
      661 aggtgttaat tgtaaagggg gttcttgtag aacttttgcc tgttctataa aagtcgccgt
      721 ttgtcgcagc cagtaatctt ccccggttaa ccacgggaaa tttttcggga acgcgggatc
      781 agcccaacgc cgcattagcc aggcaagata ataaaccaaa cgcatggcgc gtaaaggttc
      841 aatcagtccg atttcagcgg tgtcgaactc gccaaattct tcataagctt caataatagt
      901 ttccagttgc atccgctgct cggctttatc gccattgagc aacatccaca aatcctgaat
      961 agctggacca ttacgtgcat catccagatc aacaaacatt ggaccatcgc gccagagaat
     1021 attcccggcg tggcagtctc catgtagccg cagaacggtg aaatcttccc gccagtgtgc
     1081 tgtaacggcg gcgatcagct catctgtcgc tttcaggaat gccgctttca acccggaagg
     1141 tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg agatattcat tcagaccgat
     1201 ggtcgggcga tggataaaaa gctgtttgtg ccccgtctgg tgcatacgcc ctaaataacg
     1261 cccaaccgct tccgtctgat cgatattatc agcttcgaac tggcgaccac cgacgcttgg
     1321 aaaaacagcg aaataaaatc cctgatgatt caataaagtc tgaccgttaa aggccacagg
     1381 tgctgcgacc ggaacttcat catttaccag ctgcaacgca aattgatgtt cttcgaggat
     1441 ttgatcggct gtccaacgtt cagggcgata aaatttgacg acaaaacgtc gacgctcttc
     1501 gtcctgaaat tgatagacac ggttttcata gctgttaagc ggggtaagac cggaatccac
     1561 ccggatccct tgctcaaaca gagcgtccat gatggtatcc gggtgtagtg tctggaaagt
     1621 aaaagcgctg ttgttcatcc catcatccgg aaaacattac gaatgatgaa ggatatcatt
     1681 tcacgatgat tttggtggcg ccgcttacaa gcttttactc tttaattacg ccgcgagcgc
     1741 gtaacagcgc cgttttgaag tcttcttcat aatctttctg caaacccggg atcaccgcat
     1801 ctttcgcgga atcacgcatt ttcaggtgat agatcagaat gtcatccgtc aaatccgcca
     1861 gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa aaattgcaac aggttaaggt
     1921 ctggctcttt ttgccaggct ggctggagga gttcaataac ttcattcaga cgtttacatt
     1981 tcatagtgat gctccttact cttgagacag acacgttagc agggtcaatc ccacaataaa
     2041 agaggcgata tcggtgaatc tgatgacgac gataacaggc gttgtgctgg caggcggtaa
     2101 agccagacga atgggcggcg tagataaagg attgcttgaa ttaaacggca aaccattatg
     2161 gcaacatgtc gctgacgcgc ttatgacgca gctctctcac gtcgtggtta atgccaatcg
     2221 tcatcaggaa atctatcagg caagcggtct gaaagtgatt gaagattcac tggcggatta
     2281 cccaggccct ctggcaggaa tgctttcagt aatgcagcag gaagctggtg agtggttttt
     2341 gttttgtccg tgcgatacgc cttacattcc ccatgtttta gcttcccggc ttaatcatca
     2401 gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc cgactattgc
     2461 tctggtaaac cgtgctattg agcctttatt actagaatat ctgcaagcag gagaacgccg
     2521 ggtaatggca tttatgcgtc tggctggcgg ccatgcggtt gatttcagcg atcataaaga
     2581 tgcatttgtt aacgtgaata cgccagagga gcttgcccga tggcaggaaa aacgatgata
     2641 ccgttactcg cctttgccgc gtggagtggt accggaaaaa ctacgctttt gaaaaaactg
     2701 atcccggcat tatgcgccag agggatccgt ccagggctga ttaagcatac gcaccatgat
     2761 atggatgttg ataagccagg caaagatagc tatgagctgc gcaaggctgg cgcggcacaa
     2821 accatcgttg ccagccagca acgatgggcc ttgatgacgg aaaaaccaga cgaagaagag
     2881 ctggatctac agtttctcgc aagtcgaatg gatacctcaa agctggattt gattctggtc
     2941 gaagggttta agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg agccggacat
     3001 cgacctgaag aattagtgat agacaggcat gttattgctg tagccagtga tgtgccgctt
     3061 aatcttgatg tcgcattact ggatattaat gatgttgagg ggctggctga ttttgtggtg
     3121 gagtggatgc aaaagcagaa cggataagca agagtaggct ggataagacg cgccagtgtc
     3181 gca
//
LOCUS       DAZPAC010000351         3174 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZPAC010000351 DAZPAC010000000
VERSION     DAZPAC010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.358"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            532..2385
                     /locus_tag="ACHUPM_004359"
     CDS             532..2385
                     /locus_tag="ACHUPM_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR0871958.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            2536..2751
                     /locus_tag="ACHUPM_004360"
     CDS             2536..2751
                     /locus_tag="ACHUPM_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR0871959.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2756..3070
                     /locus_tag="ACHUPM_004361"
     CDS             2756..3070
                     /locus_tag="ACHUPM_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR0871960.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
BASE COUNT          778 a          761 c          885 g          750 t
ORIGIN      
        1 ttccaggccc atccgctccg gtgggccttt ttactgcaga aaacaggttc cccgttaaat
       61 gctatgttgc tcacaattca gtaagttgac agttgcctgt cagactgggc atttgttaaa
      121 aaaatttcgc atggtgaatc cccctgagcg gaggggcgac tggtgacggt ataatctctg
      181 attatcaaaa cgagaatgac gcgggtttag tggcaccggg ctgaactcac cgggaggcac
      241 ccggcaccat gtgcatgatg atacagatac gcggctttag cccctctccg gaggggtttt
      301 cttgtgggca aaaaaagccc gcgctgggag acgcgggcgg caaggaataa acaataaaac
      361 gtgaagtaat atttcagctg gcgaataata ccccatagta atcactctgc gcaactgcgc
      421 ggtctttttc gaattgcggg ctgtcgtctc tcttctgcca ttgtcctgta acttccggac
      481 ttcagcccgc tccttatttt actcacaata ttatcccggc cgggaggatt catggcattt
      541 aaacactatg acgtggtcag ggcggcatcg ccgtcagacc ttgcgaaacg actgacacaa
      601 aaactgaagg agggctggca gccgtttggt agtccggtgg ccataacccc ttataccctg
      661 atgcaggcga ttgcagcaga aggtgatgtg gtcgtcagtg gtgcaactga gccggagtgg
      721 tactacgtca tcgtactggc cgggcaatcc aatgccatgg cttacggtga aggtcttccg
      781 cttccggatt catacgatgc gccccatccg cgcattaagc aactggcccg tcgtaacaca
      841 gtgactcccg gtggtgaagt atgcgtattt aacgacatca ttcctgctga ccattgtctg
      901 catgatgttc aggatatgag tacgattaac catccccggg ctgacctgag caaagggcag
      961 tacggctgtg tcggacaggg cttacatatt gccaaaaaac tgcttccgta tatccctaat
     1021 aatgcgggga tcctgctggt accatgctgt cgtggtggtt cggcattcac ccagggcacg
     1081 gaggggacat tcagcgagtc cacgggggcc agtcaggatt cggctcgctg gggagtgggt
     1141 aagccgttat atcaggatct gcttttccgc acgaaggcag cattgcagaa aaacccgaaa
     1201 aacgttttgc tggcgatatg ctggatgcag ggggaattcg atatgacgaa tgccagttac
     1261 gcccagcagc cagcagcatt tcttgcaatg gtacagcagt tccgtgctga ccttgccggg
     1321 ctggcggcgc agtgtcacgg tggaagtccg gcatcagtcc cctggatttg tggcgacacg
     1381 acatacgcgt ggaaacaaga acacggtacg caatatgaag tggtatatgg tgcatataaa
     1441 ggtaaagaat cccagcagat ttattttgtt ccctttatga ccgatggtag cggagttaat
     1501 acaccgacaa acaacccgtc agaagatcct gatattgccg ggtctggtta ttacggttcg
     1561 gcatcccgaa cgaacaaaaa ctgggtatca tcaaatcgcc cgacgcattt cagctcatgg
     1621 gcgcgtcgtg gcattattcc cgatcgtatg gcaactgcta ttctgaacgt agccggtcgc
     1681 accttagcct tcattagtgg taaggcaccg gaaatcaaac cctcgcccgg cggcgacacg
     1741 ccatcggggc cgtctgaaga tgcatccata cgcacaatct ccctgttgcc gacagccgga
     1801 gacgctgctg cgcagggctg gagcattaag aatggcggag ttcagttgtc agatggtgta
     1861 tttaagatca ccaagcagag caataaagcc tggtccctga cgcgcccggt ggatgacgca
     1921 gtctccctgc tgacacgggg tggcagactg agctgtaagt ttcgactgtc aggcgcactg
     1981 accaacaacc agttcggtct gggaatttat ctgtataccg atgtagcgtt acctgacgtc
     2041 gtggcgatga ccgggactgg taacccgttc ctgatgtcgt tcttcaccca gaccacagac
     2101 ggcaaactga atctgatgca tcacaagaaa gcaggaaaca caaagttggg cgagttcggg
     2161 aattacagta acgactggct gacgctggag ctggtgttca ccgccggcag tgccacggtt
     2221 actccgaaac tgaatggagt ggctggcccg gcattccagg tcataaaaga cagtctgaca
     2281 ctggggctga atgcgctgac gctgacggat attaccaaaa atgcagcgta tggcgttgag
     2341 atagaaagtc tggtgctgga gataaatgca ccggcatcat cataaaaagt gagccagcca
     2401 aatggaaggt atcgttaaac tcaccggtag tgtcagtggg tcgtctgaga tgcctgcatg
     2461 agttatcaga gccatcagta cttaactggt ggcttttttt attgttgtca gcttccggat
     2521 aacgggagac ggggtatgta ccagatggaa aaaatcacaa caggtgtgtc atacaccacg
     2581 tcagcggtgg gaacgggcta ctggttcctg cagttgctgg acagggtttc cccgtctcag
     2641 tgggcggcaa taggcgtgct ggggagtctg ctgtttgggc tgctgacata tctgactaac
     2701 ctgtatttca aaatcagaga ggaccgtcgt aaggctgcac ggggagagta attcaatgac
     2761 tcaaaactat gaactgattg tgaaagggat ccgcaatttt gagaataaag ttacggtaac
     2821 tttagcgtta cgggacaaaa aacgctttga cggtgaaatt tttgacctgg acatctcgct
     2881 ggaccgtgtt gaaggtgccg cgctggagtt ttatgaggca gcagccagaa ggagcatcag
     2941 acaggtcttc ctggatgttg ctgccgggtt atgtgaaggg gacgagctgt tgccagaaac
     3001 gcgcccctgt tcagaggcgc ggtataccat aaaaattaac agttctgata actcgattac
     3061 gggttgttag ctttttgcag ttggctttcc agtatctttc attggtagca tcctgataaa
     3121 tatccatgag cgcaaaaatc aaatacggcc tgtcagctgc tgttctggcg ctga
//
LOCUS       DAZPAC010000352         3174 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZPAC010000352 DAZPAC010000000
VERSION     DAZPAC010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.359"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            397..1083
                     /locus_tag="ACHUPM_004362"
                     /pseudo
     CDS             397..1083
                     /locus_tag="ACHUPM_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1416..2291
                     /locus_tag="ACHUPM_004363"
     CDS             1416..2291
                     /locus_tag="ACHUPM_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFR0871961.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            2299..3075
                     /locus_tag="ACHUPM_004364"
     CDS             2299..3075
                     /locus_tag="ACHUPM_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFR0871962.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT          868 a          742 c          762 g          802 t
ORIGIN      
        1 ttcgcaaaaa ctggtcgcag aaaagtcctg ttttcagctt ggaaacccgg taatcagaat
       61 aatggatagg ggaacaaaaa tctctgtata gaacgctcca ttgagggaga gaaaacgtgt
      121 cattttgacc cgttcggtat tcagaacaca caaaaccgat cgcgggtcgc tttcgtgcat
      181 agttatgcaa cgcgctaaaa acgattctga cgcatttttt atgtttaccc tgttttttct
      241 tgtctttttg agttttttgc gtcagagagc gtctgagagg gcacgtacaa cgggagttat
      301 ggtaaatgga tcgggttttc gggaaggttc gacaggattt gccgttgggt gtagtgtaag
      361 cgactgaaaa acaaacgccc ctgaaatcat gtccagatcc ggcagaacca gcctcatgcg
      421 cagttctggc tggtttttga cgttgatcgc gagggggcag cgattgactg gagcgaccgg
      481 aacgctcccg cgccgaacat caccgtaaaa aatcctgtga acggtcatgc tcacctgctc
      541 tacgcgctca atatcgccgt gagaaccgcg ccggacagct cggttaaggc gctgaaatac
      601 gccgctgcga ttgaacgttc gctgtgtgag aaactttgtg ctgatgtgaa ctacagcgga
      661 ctgatttgta aaaatccgtt ccacctggaa tggcaggtga tggagtggcg agaggaagcc
      721 tataccctcg atgaactggc tgattatctc gatttgagcg cctcagcgcg ccgtagcatc
      781 gataaacatt acgggatggg gcgaaactgc cacctgttcg aaatgacgcg caaatgggct
      841 tacagggcga ttcgtcaggg ctggcccgaa ttctcacagt ggcttgatgc cgtgattcag
      901 cgtgtcgaaa tgtacaacgc atcgcttccc gttccgcttt caccgcctga atgtcgggct
      961 attggcaaga gcattgcgaa atacacgcac aggaacttca cgccggaaac tttcgcacag
     1021 tatgtggctg atacgcacac attaacgtac gttttcgttc cattggccct caccccccga
     1081 taacgaccca ggtagcgcgc cgtattcccc ccgccggccg ttttcttcga catgtacatc
     1141 aggcgcggaa ctcatcgggg cgcgggaatg aaatcccatt cagactccgg atagattcaa
     1201 gcaagcccac ttatcgtcaa aatcggtgtt gcaaaaacgg gagtgaccat agatccgttt
     1261 tccaagcgga ccccataata tcggctggta aacatcactc gctagtgcat tctttttgtt
     1321 ggtaatgata atcatttaca ttcattcttt gatgttttat cataattaat gttttgtcaa
     1381 taaatatatg gaaacatatt gtttcagatg ggaatatgag aaagttcatc cttatttcta
     1441 tgataacgtt agctagtgtt agtctgggag cctgcagaca gaatgtcacc ataaagcagg
     1501 atgcgccagg gcagaaagct ttcctgactg acgggcagat ttacgatttg cacagtggca
     1561 aaattatttc tagcagcgag cttttggcgg atcttgctac cgctcgacat cttattattg
     1621 gtgaaaaaca cgataatgcc gagcatcatc agattgagct ttggcttatt caaaatttgc
     1681 tcatccagcg accacagggg agtgtgttgc ttgagatgtt gacgtcagag caacagcccc
     1741 gagttaacca agttaaatgt tggctgaaag ataatcctgt ggttcgagat agccgtgttc
     1801 aggaactact taactggcaa aaagggtggt cctgggagat gtatggcgac atcgtcatgc
     1861 agctattacg tgggccttac ccgctgctca atgccaacat tggccgggag cagatgttgg
     1921 cactgtacaa aaaaaacgaa tttcctaaag gaaaaaaatc cactgcacct gtggtacaag
     1981 aagctctgcg tgaaaccatc atatctatgc atgaaggcaa ccttgaaagc cagcaactga
     2041 cgtcaatgct ctctattcag cagcagcggg atcgctacat ggcccgccag cttctgagtg
     2101 ccccggtacc gtcgttgttg attgcaggtg gttatcacgc gtcaaaaagc atgggcgtac
     2161 cgttacacat ggaagatctt gccaccggca cccatccagt ggtgttgatg cttgcagaaa
     2221 aaggaatgaa catcacagta gatcatgctg actacgtgtg gtttgtagcg ccagatacaa
     2281 caaaaaggta aaccatggat gatgaatatt ctccacttct cacagtcggt aaaatggagc
     2341 agctggttta tctgttctct actgcttcat ggactgattt tccttgcgtt tatctggcgt
     2401 ttttccgaag ttcagcccgc tatgtctcct gcaccggcga tcatgctgca atgggcagaa
     2461 attatagagg ctccttcttc tccactttcg cttccggtgg gaatcgctca acaggagtcc
     2521 gcagtgacag aagaaaagca acaaactgag gacagacaac aacgtcctgt cactgaagac
     2581 agtgatgcaa ccattgaaat aactaggaag aaaaagtcat ccgatggaga aaagaaaaag
     2641 acacgtccac ctcgtaagat aaaggctcaa accagcgaca gcaaccccac agccgtttcc
     2701 agtaatgctg cacctcaggc tctggtcgaa tcgtcccgta ttgctgcgcc ttttaacagt
     2761 gactcaacaa agcgagataa ttctgaggca tcttgggaaa gcagagtaaa aggccaccta
     2821 aaccgttata aacgttaccc tggcgatgcc agaaaacgtg cacgaaccgg cacagccgtc
     2881 gtgactttta ccgtaaacac ggaaggcact attgtcagta gtttcttgga aatctcttca
     2941 ggaacccttt ctcttgaccg cgaagctatt gctgttttag aacgcgctca accacttccc
     3001 aaaccaccgc ctgaaatatt ggagggagga ttatttaaag taaaaatgcc gattaccttt
     3061 aagttgaagg agtaaaggga taattggccg ttcaagttca tttaactgaa atttaatttt
     3121 catttttatg ctgaacattt tatcaaactt ttccactgtc gaaaaaatca aaaa
//
LOCUS       DAZPAC010000353         3166 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZPAC010000353 DAZPAC010000000
VERSION     DAZPAC010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.360"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            43..444
                     /gene="spa15"
                     /locus_tag="ACHUPM_004365"
                     /gene_synonym="spaK"
     CDS             43..444
                     /gene="spa15"
                     /locus_tag="ACHUPM_004365"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFR0871963.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            448..1740
                     /gene="spaL"
                     /locus_tag="ACHUPM_004366"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             448..1740
                     /gene="spaL"
                     /locus_tag="ACHUPM_004366"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFR0871964.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKNRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            1821..2171
                     /gene="spa13"
                     /locus_tag="ACHUPM_004367"
                     /gene_synonym="spaM"
     CDS             1821..2171
                     /gene="spa13"
                     /locus_tag="ACHUPM_004367"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFR0871965.1"
                     /translation="MYRDVEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVK
                     KHIIQYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLI
                     LDELSQEDMKYGIR"
     gene            2158..3036
                     /gene="spa32"
                     /locus_tag="ACHUPM_004368"
     CDS             2158..3036
                     /gene="spa32"
                     /locus_tag="ACHUPM_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HFR0871966.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
BASE COUNT         1081 a          454 c          648 g          983 t
ORIGIN      
        1 tataccatta atgtattaaa gactatttag tgaggtttaa atatgagtaa cattaattta
       61 gttcaattag ttagagatag tcttttcacg attggttgtc ctccatcaat tattacagat
      121 ttagacagcc attcagcgat taccatttct ttggatagta tgcctgctat taatatagct
      181 cttgtcaatg agcaggttat gttatgggct aattttgatg caccaagtga tgtaaaactt
      241 cagtcatctg cttataacat attaaattta atgttaatga actttagtta tagcattaat
      301 gagctggtag agcttcatcg ttctgatgag tacttacagt taagagttgt tattaaagat
      361 gactacgttc acgatgggat tgtttttgca gagattctgc atgagtttta tcagaggatg
      421 gaaatcttaa atgaggtctt ataatcaatg agctatacaa aattgctcac tcaattatct
      481 tttcctaata gaatctcggg gccaatcttg gaaacaagtc ttagcgatgt ttcgattggt
      541 gagatttgta acattcaggc tggaattgaa agtaatgaaa ttgttgcaag agctcaggtt
      601 gtaggatttc atgatgaaaa aacaatatta agcttgattg gaaattctcg tggactttca
      661 cggcaaacgt tgattaagcc cactgcccag tttcttcata cgcaagtcgg ccgtggatta
      721 ttgggagcag tagtcaatcc tttaggggag gttactgata agtttgctgt tacggataac
      781 agtgaaattc tttatcgacc tgtagataat gctcctccgc tatatagtga aagggctgca
      841 attgagaagc cttttttaac aggtattaag gttattgatt ctttactcac gtgtggtgaa
      901 ggacagcgaa tggggatttt tgcgtcagct ggttgtggca aaacttttct catgaatatg
      961 ctcattgaac atagtggtgc tgatatatat gttattgggt taattggtga gcgaggtcga
     1021 gaggttacag aaacggttga ttatttgaaa aactctgaga aaaaaaacag gtgtgtttta
     1081 gtatatgcaa cttcggatta ctcttcggtt gatcgttgta atgctgcata tatagccact
     1141 gctatagccg aattttttag gactgaagga cataaagtag cgctttttat tgattcatta
     1201 acaaggtatg ccagagcatt acgtgatgtg gccttagccg ctggagaatc acctgccaga
     1261 agaggctatc cggtttcggt ttttgatagc ttacccagac ttcttgaaag gccaggaaag
     1321 ttaaaggcag gtggctctat tactgcattt tacactgttc ttttggagga tgatgatttt
     1381 gctgatccat tagctgaaga ggtaagatcc attttagatg gacatatata tttgagcaga
     1441 aatctagccc aaaaaggaca atttcctgca attgattcct taaaaagtat aagcagggta
     1501 tttacacagg ttgttgatga aaaacatcgt attatggccg ctgcatttcg ggagctactt
     1561 tctgaaatag aagagctaag gacaattatt gactttggtg aatacaaacc gggggagaat
     1621 gcctctcagg ataaaatcta caacaaaata tctgttgttg agagttttct gaagcaagat
     1681 tatcgactcg gttttactta tgagcagaca atggagctta ttggtgaaac aattagataa
     1741 ggttttaaaa atcaaagata aatatcaacg gtctgttaaa ttaattgaag cacacatatt
     1801 aacactaaaa aaaaaactct ttgtatcgag atgtggaggc attagataaa aggattatat
     1861 acttcctgca actggagaat gacttagaac ctgtaggcgc tcagagtgtt agtcagcttt
     1921 tcaatacaag acgtaagatc gctatagtaa aaaagcatat tatccaatat cagtctgaac
     1981 gaattcttct aaagggaaga attgaagaaa ttcaaaaaga tattgatgag gcaaatgctt
     2041 ctaaaagaaa actgcttcat aaagagagta aaatatgtaa acggattggc ttgattaaaa
     2101 gaaataactt tgccaaacag ttgatacttg atgagttatc acaagaggat atgaagtatg
     2161 gcattagata atataaacct aaattttagt agtgacaaac agatagaaaa atgtgagaaa
     2221 ctatcttcaa tagataatat tgatagtctc gttttgaaga agaagaggaa ggttgaaatt
     2281 cctgagtact ctttaattgc atctaactat tttactattg ataagcactt tgaacataag
     2341 catgataaag gagaaattta tagtggcatt aaaaatgcgt tcgaacttag aaacgaacga
     2401 gcgacatatt ctgatattcc agaatcaatg gccattaaag aaaatatttt gataccagat
     2461 caagatataa aagcaaggga aaaaataaat atcggcgata tgagggggat cttttcatat
     2521 aataagagtg gaaatgcaga caagaacttc gaaagaagtc atacttcttc tgtaaaccct
     2581 gataatctgc tagaatctga taatagaaat ggtcaaattg gtttaaaaaa tcatagcttg
     2641 tctattgata agaatattgc tgacatcatt tctttactaa atggaagtgt tgctaaatca
     2701 tttgagctgc ctgtaatgaa taaaaatact gcagacataa ccccatccat gtcattgcaa
     2761 gaaaaatcaa tagttgaaaa tgataaaaat gtttttcaaa aaaatagtga aatgacttac
     2821 cactttaaac agtggggggc tggacattct gttagtattt cagtggagtc tggttctttt
     2881 gttctaaaac cgtcagatca atttgtagga aataaacttg acttaatttt gaaacaagat
     2941 gctgagggta attatagatt tgatagcagt caacataata aggggaataa aaataatagt
     3001 acaggatata atgaacagag tgaagaagaa tgctaagaat taaacatttt gacgctaacg
     3061 aaaaactaca gattttatat gcaaagcaac tctgcgagcg tttttcgatt cagacattca
     3121 aaaataaatt tacaggcagt gaaagtttag tcactcttac ttccgt
//
LOCUS       DAZPAC010000354         3132 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZPAC010000354 DAZPAC010000000
VERSION     DAZPAC010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.361"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            95..577
                     /gene="tnpA"
                     /locus_tag="ACHUPM_004369"
                     /pseudo
     CDS             95..577
                     /gene="tnpA"
                     /locus_tag="ACHUPM_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
     gene            complement(800..1156)
                     /locus_tag="ACHUPM_004370"
     CDS             complement(800..1156)
                     /locus_tag="ACHUPM_004370"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFR0871967.1"
                     /translation="MIQAQEYIGAGYHWVVDLDLEKFFDRINHDVLMSRIEKRVSDKR
                     VLSLIRRFLNAGVMDAGLVRPVTEGTPQGGVISPLLSNLFLHYAFDMWMQRQCPDVPF
                     ERYADDGVPRRRAPGT"
     gene            complement(1217..1420)
                     /locus_tag="ACHUPM_004371"
     CDS             complement(1217..1420)
                     /locus_tag="ACHUPM_004371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871968.1"
                     /translation="MRVSELPDYLRHHWPELKAQLLSGRYRPSPVRRVSILKPGGGER
                     LLGIQMVVDRFIQQAMMQVLQAL"
     gene            1446..2228
                     /locus_tag="ACHUPM_004372"
                     /pseudo
     CDS             1446..2228
                     /locus_tag="ACHUPM_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            2806..>3132
                     /locus_tag="ACHUPM_004373"
     CDS             2806..>3132
                     /locus_tag="ACHUPM_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR0871969.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGD"
BASE COUNT          666 a          890 c          836 g          740 t
ORIGIN      
        1 cggcacggaa ccacggcagc ttgacggctg caatgacgtg tgtcgtctgg cggtggagta
       61 tctgctgtat gcagcccgaa aacgggggct ggaaatcggc atcttctgca ccatccacac
      121 tttacgcctt cactttgaag aacatttacc cctcgttgta gccggacgtc gcctgggcgt
      181 accaaaatca acagtttgta gtatgtttgt gcgcttccgc aaagctggcc tttcatggcc
      241 tctgcccgca ggtatgtcgg agcgggaact tgacgcccgt ctttacggga gtgcctccac
      301 agtacctgtc gtacttactg aaagtaccgt tatgcccgaa gtccccgggg taaaaaagcg
      361 tccccggcgc cccaacttcc cttatgagtt caaaatcgcc ttagtggagc agtcactgca
      421 gcccggagcc tgtgtggcgc agattgcccg tgaaaatgga atcaacgata acctgctctt
      481 caactggcgc catcaatacc ggaaaggtgg cctgctgcct tccggaaaaa atatgccggc
      541 actgcttccc gtgacgttaa cgccggagcc ggataaccat ggttttgaca tcatctatat
      601 gctttcgaca catcatcagc ggttcacttt cgttcgtctc ttcgatccat acctgatcgg
      661 ctcacgtccg accttttccc atctcgctca ccaccacatc tcttaaatgc agcagcagtg
      721 ggtggtttgg tgcctgcgcc tgcacgccga caccgagggg ccttccctca tctcttgtgc
      781 agcatggctg cacagggact cacgtccctg gtgcgcgcct tcgtggcaca ccatcatcgg
      841 cgtacctttc gaagggaacg tcgggacact gacgttgcat ccacatatca aaggcatagt
      901 gcaggaacag atttgagagc aggggcgaga tcacgccgcc ctgcggcgtt ccttctgtca
      961 ccggccttac cagacctgcg tccattaccc ctgcattcag gaacctgcgg ataagtgaca
     1021 acacccgttt atccgatact cgtttctcta tccggctcat cagcacgtcg tgatttatcc
     1081 ggtcgaagaa cttctccaga tcgaggtcga ccacccagtg atacccggcc ccgatatatt
     1141 cctgggcctg tatcactgcc tggtgggccg aacgaccggg acggaacccg tagctgttgt
     1201 cactgaacga gctatctcac agtgcctgca atacctgcat catagcctgc tggatgaagc
     1261 gatccaccac catctggatg cccagcagac gttcgccgcc gccgggcttc agtatggata
     1321 ctcttctcac aggggatggg cggtatctgc cggacagcag ttgcgctttc agctctggcc
     1381 agtggtgcct caggtagtcc ggcagttcac ttacgcgcat gccatcgact cccgccgcac
     1441 cttgattgcg tcttgtcgat ggaacagcaa tcagcgcgcc cgggggcggc agcgctgaat
     1501 ggcgactaca tatgggatat gatcctcata cctgtcagtt cactgatttt gagctaaccg
     1561 acagcagaga cgctgaacgg ctggatcgat ttgcgcaaac ggcagacgag atacgaattg
     1621 ctgaccgggg attcggttcg cgtcccgaat gtatccgctc acttgctttt ggagaagctg
     1681 attatatcgt ccgggtttac tggcgaggat tgcgctggtt aactgcagaa ggaatgcgct
     1741 ttgacatgat ggattttctg cgcgggctgg attgcggtaa gaacggtgaa accactgtaa
     1801 tgataggcaa ttcaggtaat aaaaaagccg gagctccctt tcctgcacgt ctcattgccg
     1861 tatcacttcc tcccgaaaaa gcattaatca gtaaaacccg actgctcagc gagaatcgtc
     1921 gaaaaggacg agtagttcag gcggaaacgc tggaagcagc gggccatgtg ctattgctaa
     1981 catcattatc ggaagatgaa tattcagcag agcaagtggc tgattgttac cgtctgcgat
     2041 ggcaaattga actggctttt aagcggctca aaagtttgct gcacttggat gctttgcgtg
     2101 caaaggaact tgaactcgcg aaagcgtgga tatttgctaa tctactcgcc gcatttttaa
     2161 ttgacgacat aatccagcca tcgctggatt ttccccccag aagtgccgga tccgaaaaga
     2221 agaactaacg cgttgtggag aataacaaaa atggtcatct ggagcttaca ggtggccatt
     2281 cgtgggacag tatccctgac agcctacaaa acgcaattga agaacgcgag gcatcgtctt
     2341 aacgaggcac cgaggcgtcg cgttcttcag atggttcaac ccttaagtta gcgcttatgg
     2401 ggtaaatccc cgcctttttc ctgccggtcc ccggacagag cacaaaggaa gtcgccgccg
     2461 gctccgcttt acccggccat gcggggcatg gccttgtggg ttttcagctc tgtagcctca
     2521 gcgtcgtgtg cgggctgtgc cgtgcctcca tcttagccgg gctggcgttg atgcaagggt
     2581 acgcttcgcc gctgcggtca cccggtccct ccttcccgtc tgccgtgatt ttccggtcgt
     2641 tcacccgctc agatttcgcg gtttcaccct ccaactcccg ccagccgtgt gcgtcaggct
     2701 gcggcttccc ttgcgccctg tgcatccccg ccttatcgcc cggctaagat ggaggcacgg
     2761 caccgcccgg tgtcgcggaa tatgtgaaca acggaggaaa acatcatgca atatgcgaaa
     2821 cctgtcactc tgaatgttga agagtgcgac cgtctctcct ttctgcctta cctgtttggc
     2881 aacgattttc tgtatgccga ggcgtatgtg tacgcgctgg cgcaaaaaat gatgccggaa
     2941 tatcagggcg gcttctggca cttcatccgc ctgccggacg gtggcggtta catgatgccg
     3001 gatggcgacc gtttccacat ggtgaacggt gcaaactggt ttgaccgtac cgtgagtgct
     3061 gatgccgcag gcatcattct gacttctctt gtgattaacc gccagttgtg gctgtaccac
     3121 gacagcgggg at
//
LOCUS       DAZPAC010000355         3112 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZPAC010000355 DAZPAC010000000
VERSION     DAZPAC010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.362"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..1258)
                     /locus_tag="ACHUPM_004374"
     CDS             complement(<1..1258)
                     /locus_tag="ACHUPM_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR0871970.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLIERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVFPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLLN"
     gene            complement(1415..1624)
                     /locus_tag="ACHUPM_004375"
     CDS             complement(1415..1624)
                     /locus_tag="ACHUPM_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871971.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1647..2357)
                     /locus_tag="ACHUPM_004376"
     CDS             complement(1647..2357)
                     /locus_tag="ACHUPM_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0871972.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2873..>3112)
                     /locus_tag="ACHUPM_004377"
     CDS             complement(2873..>3112)
                     /locus_tag="ACHUPM_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871973.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          913 a          653 c          507 g         1039 t
ORIGIN      
        1 aattaagtaa attagtcagt gaccttgtaa agtctgttaa accttcctgt gaaccatcga
       61 acattaaggc cagattatta ttccatgaat tacttaagcg gcctaactgc gtctgagtag
      121 agttaataat cttttcccat ccaccagctt tatcaatcat ccttcccatt tcagcggata
      181 ctttaggaaa aacatcttta gaaagaacct taccgtcttt catggccttg tctaattctt
      241 ccgtagtcat cccaagactt ctggcaaata actgaaaagc acctgccatt ctgtcaccta
      301 attgaccgcg tagctcttca gcctgaatct ttcctttaga tgccatctgc tcgaaagctt
      361 ttaaaatccc gttaatttca tcggtgctta gccccattaa tctggatgta ccaatcacat
      421 tagaaaataa ggattctttc tggtctgcat tcatatcacc agcaccagcg gaaaacttag
      481 caaaactgct taatgtttca atctggtcta caccactact atcagcaaga ttcttaacaa
      541 attctgtagc tcgtttagca ccttcatcac ctaaaacgaa ttgtgccgct cgttgtgatg
      601 cttctttttt cagaccaacc tccataatcc gcctgtaagc ttctaaggct gtctgaaaac
      661 tataaatggc tgtacctgct ttgattgcac tcgtagctaa tacatccaga ctatttgaag
      721 cattttcact gtgattattt ttgctggtgg tcttgttaaa cttagaaagt gtttcacgaa
      781 gagactgatt taactttgct aactcacgat taaaagtttc gtttccaagt gtcttggatt
      841 tactgcttaa gctgtaatac tggcttttaa aatacttctc aagcatagga ttaaagtcac
      901 gaagcttgct catattcgct gagtaagctg ctgtgattgc tctgcttccg ctaattcgtg
      961 gctgtcttgg ttgtctaagt ggctttaaag tatcctcaat ttcagtttta atcttaaacg
     1021 ctctagtgtt tttaagacgc tgctcaatct tggaaacagt tttatcgaca tttctttcta
     1081 ttagtttgag agaatcattt aaaggcgtag gattaactct aaacgactta ttcaatcgct
     1141 tctcaattcg tgatgctacc tgcatcgttt gtttctcggc tttttccaag ccttttaaaa
     1201 ctttctgaga gttaaaactt aactcaatca gaaaatcatc aactttaaat tgtgccattc
     1261 atatttttca cctctttaag cgtgttgtac gtaaattcta tttttcagtg tgggtattag
     1321 ctttacagat ttcacgtagc acacatacaa aaaaagggag agctaccgat gtcactcgat
     1381 aatctctccc cgtatttacc caaccaattc ttttttaatg tcggacaaga taatgtaatt
     1441 ccgtttcacc tatgatttga gcatcagaag gaatacggtc aatacaccac tttggaatct
     1501 tgcgtgtttt tggtttgtgt gaggtgataa cttgacgttt gtttttaatt cttaaggctg
     1561 gcttagtttg taactttaag ttttcacaaa cagaccgaat agtctctaaa cgttcttcaa
     1621 acattaatca cctctttaat tcataatcaa acagccttgc tatacttctg agaaatcttc
     1681 gttaactgct caatatcagc catcaaagca atcttcttct gcgactcata ttcactaaca
     1741 agttctttca tagtatcaag ttctttttta agcttttgct ttaacttatc cagtttaccg
     1801 gaataatgct tagtcaaatt agcttcagct aactgaagaa tctcttcatc tgttttatca
     1861 gtcatcagaa taaattgacc agaacccctt aatgcttgtc tgacatcaat taaccggaac
     1921 ccttcaggga tactttgaag aactttagga acgtcttcaa tcgctgtacc aaaaccatca
     1981 ttaaagtttc cttcataata gataaacggg tttgacttaa tctcgtaagc aatatcatat
     2041 tctgaaggtt tcgaggtttc ttcgaagttc ttaacagcat cgtaagcgac atcaagtcta
     2101 agacgctgtg attttaaagc tgggtaatat tcacgctcaa gctcaaatag tgcttcagtt
     2161 ttgcagatat caggcatttc ttgaggctca ggagacacaa aggaaattac ctttggtgac
     2221 atctttagct cttgctcaag ctcaatgcgc ttgtcatgct cttgacgctg aatagtatgt
     2281 agcggatgtt caccctgtgg tgaccagtct ccgaagctat caaccttttt accgttaata
     2341 tataaatcgt ttgtcattat aatttcctct aagtgtggtt atttcttaat agagcacctt
     2401 caacgccaat acacttagaa gatgacttaa cgatttatta aagatgctct attaagaaaa
     2461 gccctaaaca taatctgtga agggctttgg aggtttgctt ataacgttaa cgaagtttca
     2521 ggaaaacttt ctttaaaagc tctgtttaaa aactcttaaa gaaagcccta actcatgatt
     2581 agggcaaagc tgtaaaggat tcaataatga atgaacaaag actttttgag atagcctctc
     2641 agaccaacct gaaaggctag gacgaaaagc attgcacctt tccaactcag aatatccatt
     2701 catcggatta ttgaaagaaa aaataagaga gaattgctct cccccatata tacaattatg
     2761 tcatgctcaa ggttgtttgt caacagcctt taacagattg gtaacattta tactttaata
     2821 gatgcacctg caatcatacc aatcaataca aaacagataa tcaccaattc cattaatgca
     2881 ccaccttcga atccgtcacc agaatattat cgctatacaa aattgctata gccgccttga
     2941 gatatttttc agggctaacg ctgtatttgt tcgtttcttc gataataaga cgcttcatgt
     3001 ttgagtcggt aacaggaaac ataaaacgcg gttcaccagt ttcaatttcc tgaataaagt
     3061 cttcaatttc ttctgagttt tcagccatta gctttagtaa tttatcaagg tc
//
LOCUS       DAZPAC010000356         3090 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZPAC010000356 DAZPAC010000000
VERSION     DAZPAC010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.363"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..929)
                     /gene="virK"
                     /locus_tag="ACHUPM_004378"
     CDS             complement(<1..929)
                     /gene="virK"
                     /locus_tag="ACHUPM_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR0871974.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNS"
     gene            complement(934..2022)
                     /gene="capU"
                     /locus_tag="ACHUPM_004379"
     CDS             complement(934..2022)
                     /gene="capU"
                     /locus_tag="ACHUPM_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR0871975.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            complement(2025..2867)
                     /locus_tag="ACHUPM_004380"
     CDS             complement(2025..2867)
                     /locus_tag="ACHUPM_004380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR0871976.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
BASE COUNT          940 a          704 c          569 g          877 t
ORIGIN      
        1 gagtttacag gatatgcatc caaagagcgg atagcagttt catggatata atccaacaaa
       61 gcgtatcgtt ttcgatactc agagcgtttt ttactagcta tattgcttag cggctttcga
      121 accacctgtg ttggtaattt gtaccagtca ccacaggttt tccctgctac agactcccag
      181 aaatcactgt agacagcaac aaaggttttg cgtttctgat accagtatct caattgccgg
      241 aaaacatgac tgtgctcact gactgccaga cactcagaga cattacaagc tttcatcaac
      301 gcacaaaatg cttcaaaaat aatccgtttt gggaaaagcc cataacaggc ccgggttgcg
      361 caccgaataa catcaggtcc ggtatttttg ggggctccct gtaatccgcc aataaaagcg
      421 gtgtgtccat tttgagtcag aatgactgaa aatgttaacc gggcaatcac tattttattg
      481 aaaaacaatg acaacatcaa ctctccttcc cggtcaaaac cactgggaga acactggata
      541 tcaaaatcct ctccatcttt cccgatgaat tggactaatg ataccgattt ttgtggaaac
      601 aaaaattcag aatatttttc tggcaaattt tgcacaaagc gataatgctc aagaatatat
      661 tgcccccggg accaggctcg ccccccttta tgcaaataag gtcgatgaat tcttgctggc
      721 aacgtgggtt gtattgccag aatatcctca atgcactgca aggaacgcag ttcgtaataa
      781 tatcgaatac tggcaaccgg atttatagca catcgcaaaa tgtacatgaa ccggaatttt
      841 ctgtgttccc acttccccgg gagtgaatct gaggaaaaaa caatcctttt aaggaaaccg
      901 ataaatgata agttacttac agaaaacata ttgttacttg tgcttcgcta atgtgagtat
      961 ttttaacgca gttttattaa tatcaaaccg ctcttctata tcctgttttg cctggcgagc
     1021 catctgataa aaacgcccag ggtcattaaa aaaatcattc agggcgcaca tccatgcgtg
     1081 cttattacct gctggtaaca atgtcccggt ctggttgttc tgaataacat cagggattcc
     1141 accaatctga ctggccagta caggcacaga aaatgccgat gcttctgcca gtaccatacc
     1201 aaacgattcg ttttctgaag gcagaaccac cagactggca acccgatata cgggggcggc
     1261 aggaaaaaca ttgtcagcaa taaaaacatc atcatgcatg cccatactgt caatctgata
     1321 ctgtaaatgc tcccgcagtt caggcgaacc agaccccaca atcaaccaac agaactgtcg
     1381 tccattcatt tttaaatgga acagtaaatt caacataaat tcgtgccctt tttccgggcg
     1441 caacattcct acctgagcaa taacggggca tccccttcgg gacgccagcc aagaaagaac
     1501 atttggaggc aaactgtttc gggaatcaac gtataatttc tggaaatcaa agcctggagg
     1561 cacaacagta acccgggttc gacatccttc ctgctccaga tgtgtcttca tacttgttcc
     1621 gggaacaatc acctcatcgc aaaaatgatt tattgaaaaa acttttgttt ttcgtgtcaa
     1681 atatgtcttt tgcctgataa ttctgaatgg atgtttccaa gtgaataacc gtactaaacc
     1741 aacaatattg ctatcatgcc cactgtgaca aacgattgca ttgggctgaa aaccatgaac
     1801 tattccgagt aatctccatg cagtagggat gtgaagactg tttcgaaata acgcgaatgt
     1861 gatatcaatt cccaatttac tggcttcaaa agcaattttg ctgttttccc tgcagacaag
     1921 cagaactgaa tgccccattt tcttcagggc cttcatttga gcaacagcct gtaattcctg
     1981 accacctata ttcggtgatg attccgtaaa tagtatattc attattaatc atccggtaat
     2041 cttggcccct tatgaaaggc cagcaacgat gaaaaaaacg gtgttgtata ataaaaaagt
     2101 cgtcttttca gccatgcgca actttcccgt tctttggtgc ttattcttcc tatccgtgta
     2161 gttcctttag caggagcatt cattcttctt tctgttgtat ataaataata gaatccaagt
     2221 tcttcagcga cctgaatata atccttgtta taataacctt ccggccaaca caaatgttta
     2281 ctgcattttc ctgtcatttc tttcaaatat tctctcccgc tcaacaagtc ctgccgaaga
     2341 tgtttacact gttcttctcg tgaactgaac tttttatccc agcgtgtatg agtatgcgta
     2401 tgaacatgaa attcgaccag accagattgc aacatttcgt ttacttcaga ccagcgaagc
     2461 ataacattat cagcattccc tgtcgctatt tgatgttcgc aatcgcgatg agaatattcc
     2521 tttccaggag agtgacgaac cggaccattc ccaataaaac cggtaattaa aaaaatatgt
     2581 gctttgagat taaactcttt taataaagga tatacctgaa accagttgtc aaggtatcca
     2641 tcatcaaatg tcaacataac gcttttccgg gggagttttc cgccccgata aaaaaactcc
     2701 agttcatctg atgacagtgt tttccagttg ttttcagcca accatttcat ttgcttccgg
     2761 aatgtcacgg gagaaagtgt aaccagtccg gggcaatgac tgacatgatg atacattaat
     2821 accggaacat gattggcttt gaacaaaatg cccccttcgt ttaacattta aagtttctgg
     2881 cacctagtaa gcagagacaa aaggaaaacc caataaagct ggtgcatttt atttccagat
     2941 actggatgaa taacatagca aacccatggc atatttgata taatttacta aattaaacat
     3001 caacaattca taaacaatga tatattatca tatcttttaa aaacgactca ctaagaacct
     3061 tgaggtggca cactgaattt ggctacctga
//
LOCUS       DAZPAC010000357         3045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZPAC010000357 DAZPAC010000000
VERSION     DAZPAC010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.364"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..172
                     /locus_tag="ACHUPM_004381"
     CDS             <1..172
                     /locus_tag="ACHUPM_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871977.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            185..1036
                     /gene="hyi"
                     /locus_tag="ACHUPM_004382"
     CDS             185..1036
                     /gene="hyi"
                     /locus_tag="ACHUPM_004382"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFR0871978.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            955..2133
                     /gene="allB"
                     /locus_tag="ACHUPM_004383"
                     /pseudo
     CDS             955..2133
                     /gene="allB"
                     /locus_tag="ACHUPM_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2190..>3045
                     /locus_tag="ACHUPM_004384"
     CDS             2190..>3045
                     /locus_tag="ACHUPM_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFR0871979.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          696 a          710 c          836 g          803 t
ORIGIN      
        1 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttcttgagcg
       61 tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc
      121 cgataacgca gtggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata
      181 aataatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg attttctcgc
      241 ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga
      301 ctacgacatt gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca
      361 caatctaccg gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg
      421 tgaagaagag tttcgggatg gcgtagcagc agcgattcgt tatgcccgtg cgctggggaa
      481 taaaaaaatt aactgtctgg tcggtaaaac gccggctggt ttcagcagtg aacagattca
      541 cgcaacgctt gtagaaaacc tgcgttatgc cgcgaatatg ctgatgaaag aagatatttt
      601 attactgatt gaacctatta accattttga tattcctggt ttccatctca ccggaactcg
      661 gcaggcgctg aaattgattg atgatgttgg ttgctgcaat ttgaaaattc agtatgacat
      721 ttatcacatg cagcggatgg aaggtgaatt aaccaacacc atgactcagt gggccgataa
      781 aattggtcac ctgcaaattg ctgataatcc gcatcgcggc gaaccgggga ccggagaaat
      841 taattatgat tatctcttta aggtaatcga aaattctgac tacaacggtt gggttgggtg
      901 tgaatataaa ccccaaacca ccacggaagc cggtttacgc tggatggatc cgtactttct
      961 gaaccgggtc gtagccactg ggaaggttat gaaaccggta ctcgcgcagc ggcaaaaggt
     1021 ggtatcacca ccatgatcga aatgccgctc aaccagctgc ctgcaacggt tgaccgcgct
     1081 tcaattgaac tgaagttcga tgccgctaaa ggcaagctga ctatcgatgc ggcacaactc
     1141 ggtggcctgg tgtcttacaa catcgaccgt ctgcatgagc tggatgaagt gggcgttgtc
     1201 ggcttcaaat gcttcgttgc gacctgtggc gatcgcggta tcgacaacga cttccgtgat
     1261 gtaaacgact ggcagttctt caaaggtgcg cagaagctgg gcgaactggg gcagccggtg
     1321 ttggtgcact gcgaaaacgc gctgatctgt gacgcaccgg gcgaagaagc gaaacgtgaa
     1381 ggtcgcgtaa ctgcccatga ctatgtggct tcgcgtccgg tatttaccga agtggaagcg
     1441 attcgccgcg tgctgtatct ggcgaaagtt gccggttgcc gtctgcacgt ttgccatatc
     1501 agcagcccgg aaagtgttga agaagtgact cgtgcacgtc aggaaggtca ggatgttacc
     1561 tgtgaatcct gcccgcatta ctttgtactg gataccgatc agttcgaaga aatcggtact
     1621 ctggcgaagt gttcaccgcc gatccgcgat ctggaaaacc agaaaggcat gtgggaaaaa
     1681 ctgtttaacg gtgaaatcga ctgcctggtt tccgaccact caccatgccc tccggaaatg
     1741 aaagccggca acatcatgga agcatggggc ggtattgccg gtctgcaaaa ctgtatggac
     1801 gtgatgttcg atgaagcggt acagaaacgc ggaatgtctc tgccaatgtt cggcaaatta
     1861 atggcgacta acgcagcaga tattttcggt ctgcagcaaa aaggccgtat cgccccagga
     1921 aaagatgccg acttcgtctt cattcagccg aatagcagct atgttcttac caatgacgat
     1981 ctggaatatc gccacaaagt cagcccgtat gttggccgta ccattggcgc gcgtatcacg
     2041 aaaaccatct tacgtggtga tgtgatttac gacattgaac agggcttccc tgttgcgccg
     2101 aaaggtcaat ttatccttaa acatcagcag taatctggcc cctgcaatgc ccgtccttgc
     2161 ggcgggcatt ctccggttaa ggtgtgttta tgttcaattt tgcagtcagc cgcgaaagcc
     2221 tgttatcagg atttcagtgg tttttcttta ttttttgcaa cacggttgtg gttcctccta
     2281 cgctactttc tgcttttcag ttgccgcaaa gtagcctgct tacgctcacg caatatgctt
     2341 ttcttgctac cgcactggcc tgcttcgctc aggcgttctg cggtcatcgt cgcgctatta
     2401 tggaagggcc gggtggcctg tggtggggaa ccatccttac tatcaccctt ggagaagcat
     2461 cgcgcgggac accgatcaac gatatcgcta ccagtctggc ggtggggatt gcactctccg
     2521 gcgtgctgac gatgttgatt ggttttagcg gattaggcca tcgcctggca cggttattta
     2581 cgccgtcggt gatggtcttg tttatgttga tgctgggcgc acagctgacc actatctttt
     2641 tcaaaggtat gctcgggctg ccgtttggca tagccgaccc atattttaaa attcagttac
     2701 caccgttcgc gctctcggtg gcggtgatgt gcctggtact ggcgatgatt atctttctgc
     2761 cgcaacgttt tgcccgttat ggcctgctgg tcggcaccat aaccggctgg ttgttgtggt
     2821 acttttgctt tccttcttcg cattcgctct ccggtgagtt gcactggcag tggttcccgc
     2881 tcggcagtgg cggtgctttg tcgccgggaa ttattctgac ggcggtgatt acaggtctgg
     2941 taaatatcag caatacctac ggtgcgattc ggggcacgga tgttttttat ccgcagcagg
     3001 gcgcggggaa tacgcgttat cgtcgcagct ttgtggcgac cggat
//
LOCUS       DAZPAC010000358         3031 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZPAC010000358 DAZPAC010000000
VERSION     DAZPAC010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.365"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            68..400
                     /gene="hdeA"
                     /locus_tag="ACHUPM_004385"
     CDS             68..400
                     /gene="hdeA"
                     /locus_tag="ACHUPM_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR0871980.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            516..842
                     /gene="hdeB"
                     /locus_tag="ACHUPM_004386"
     CDS             516..842
                     /gene="hdeB"
                     /locus_tag="ACHUPM_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR0871981.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            906..1553
                     /locus_tag="ACHUPM_004387"
     CDS             906..1553
                     /locus_tag="ACHUPM_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFR0871982.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1595..2125)
                     /locus_tag="ACHUPM_004388"
     CDS             complement(1595..2125)
                     /locus_tag="ACHUPM_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR0871983.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2281..2847)
                     /gene="slp"
                     /locus_tag="ACHUPM_004389"
     CDS             complement(2281..2847)
                     /gene="slp"
                     /locus_tag="ACHUPM_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR0871984.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          878 a          601 c          668 g          884 t
ORIGIN      
        1 aggttataac ctcagtgtcg aaattgattc gtgacggctc tttcacttta tagttgagga
       61 tattacgatg aaaaaagtat taggcgttat tcttggtggt ctgcttcttc tgccagttgt
      121 gagcaatgca gcggatgcgc aaaaagcagc tgataacaaa aaaccggtca actcctggac
      181 ctgtgaagat ttcctggctg tggacgaatc cttccagcca actgcagttg gttttgctga
      241 agcgctgaac aacaaagata aaccagaaga tgcggtttta gatgttcagg gtattgcaac
      301 cgtaacccca gctatcgttc aggcttgtac tcaggataaa caagccaact ttaaagataa
      361 agttaaaggc gaatgggaca aaattaagaa agatatgtaa ttccgggaat gcgttacatc
      421 gtacttcctt gcatattgaa caggccggaa tatcttcttt aaaagcagct attcctcctg
      481 ttcatatata atctctatat tgaatgggtt acaaaatgaa tatttcatct ctccgtaaag
      541 cgtttatttt tatgggcgct gtagcggctt tgtcactggt gaacgcacaa tctgcgctgg
      601 cagccaatga atccgctaaa gatatgacct gccaggaatt tattgatctg aatccaaaag
      661 caatgacccc ggttgcatgg tggatgctgc atgaagaaac agtatataaa ggtggcgata
      721 ccgttacttt aaatgaaacc gatctcactc aaattcctaa agtgatcgaa tactgtaaga
      781 aaaacccgca gaaaaatttg tataccttca aaaatcaagc atctaatgac ttgccgaatt
      841 aatgaggtac aagtaaaaag gagtagcaag ttgagccatc ttgctgctcc tttttgcatt
      901 tttatatgac agcagaattt attatacgtc ttatactcgc ggcaattgcc tgtggcgcta
      961 ttggcatgga aaggcaaatg cgcggcaaag gagcagggtt acgcacacat gtattaattg
     1021 gcatgggaag cgccctgttt atgattgttt cgaaatatgg ttttgctgac gtgctgtctt
     1081 tagatcacgt cggactcgac cccagccgta ttgctgctca ggtggtgacg ggcgtcgggt
     1141 ttatcggtgc aggtaacatt ctcgttcgta accaaaatat tgtaggtctg acgacggcag
     1201 cggatatctg ggtgaccgcc gccataggta tggttattgg cagcggtatg tacgaactgg
     1261 gtatttatgg ttcagtgatg accttgttgg tgctggaagt cttccatcaa ttaaccttcc
     1321 gcctgatgaa caaaaattac catttacagc tgacattagt gaacggtaat atggtatcaa
     1381 tgctcgactg gtttaagcag caaaaaataa aaactgattt agtttcgttg caggaaaatg
     1441 aagatcatga agtggtcgcc attgatatcc agcttcatgc cacaacgtcg atagaagacc
     1501 tgttgcgatt attaaaaggt atggcagggg tgaaaggcgt ttcaattagc taatctgtgt
     1561 ggcagcgtag ccagactcac cgtaagcctg aaattcacac cagataatca atatgctgat
     1621 ggcgtaccag ctcattgatc cgcttaagat tgaggatcat catgatattg tgtttatggc
     1681 agtagaacgt ttttagcgat attttcagtt tctccagaat atcttcagtt gtcatttgat
     1741 tcgcaatgca gctcagaacc tgatgttgct tgcgcgaaag tgacacattc gcttcaggca
     1801 cagagcagta actgccattg atgagtgcga caaaatcaat gatagtgata gtccgcggaa
     1861 caaaggtcac cggtactttc cccagacagc gtttaatatt aaagggggag aaaacaatga
     1921 catggacagg ttttaatcgt tcaatttgag tgagaaaatt agaatgaaat acattattca
     1981 ttaatgtatc aataatgacg aaggcattct gatgcaacat tacgtcgatc tcttcttcgt
     2041 tctgtatatg aacgacatta cctttactca gaatgttttt catcgcggtg aagaacatcg
     2101 tatccctggt aattataaga aacataaccg actcctggtg cggacattaa ctttataatc
     2161 aatttgccaa aggtaattaa caggacaggg cttataataa taatagttag cttactgtat
     2221 attaatttta tgtaatacat gatgcagatg ctgagaaagc atctgcatct ttcggtggtg
     2281 ttatttgacc agctcaggtg ttacctgact caccgcattg gtgtagtaag gcgcacccca
     2341 gcccggttcc ggccagaatg caccatagcc gtaatcccac aggttatagg tggtattaac
     2401 cacttctctc aaatgccaca cctggatgcc ctgcatattc acttccagga agttatacgg
     2461 gactttattg ataaagccag gttgttcacc ctgaatggta ccgaggatgg taacaaagtg
     2521 attacgatag ttcactgggt caaggaagcc gctttgtctg gcgagcagtc ggccctgata
     2581 gttggcttca atatcaggtt tcgcatagct atccaacggt aatacagcga tttctaacaa
     2641 cgtatccgtt ttgccattga taacgttgat aaccttccca ccaaagcgcg cttgttgacc
     2701 aacatataac cccggctggt tatgaacagc aacaaaactt ttttgaatat caggttggtt
     2761 attgcctttg atattttgcg gaattgaact acatgcggca agcaaaaatg aaaggctgag
     2821 gatgagtgca ccttttgtca tgttcatgtt actatcctta tcaacaaata ttaaaaccat
     2881 aatagatatt tctttatagt tttcatctga ttctgaggcg atatgcctga tagtaacgta
     2941 aacctgtctg atgtttatat cccttttgtt tagttcaatt ccattgattt tggggcgtgg
     3001 catgagtatc catcgcccca taatataatc c
//
LOCUS       DAZPAC010000359         3025 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZPAC010000359 DAZPAC010000000
VERSION     DAZPAC010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.366"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..294)
                     /locus_tag="ACHUPM_004390"
     CDS             complement(<1..294)
                     /locus_tag="ACHUPM_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFR0871985.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSI"
     gene            complement(306..>3025)
                     /locus_tag="ACHUPM_004391"
     CDS             complement(306..>3025)
                     /locus_tag="ACHUPM_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001745227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0871986.1"
                     /translation="SRNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFT
                     DCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGD
                     LTAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYD
                     PVDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQ
                     WQYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAY
                     SEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAY
                     ELATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVH
                     TTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAE
                     KTDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRV
                     WRRERDLTGWMSLSRKPEVTWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETEN
                     GEQAKARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTA
                     EQMAAQLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQH
                     LQQSLRLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFI
                     DSLGLKFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSN
                     NMTIYWNSRASLFCSTELNSKSQSPALGLGHEFTHAQYCLLDKENFMALLSRTDKKYE
                     NKEEARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
BASE COUNT          584 a          959 c          709 g          773 t
ORIGIN      
        1 gatactaccc ccccatctta tattgttata tttttctttt ctgaaatact tcatttgcaa
       61 taaatcaatt atcttaccag cataaagatc tctaatttca ttcaccacca ctttatatct
      121 gtatatttca ttaagacctt ccttgctaag cggaactaac gtggtaacat acgatggaag
      181 attaattatc tctatcgttt tcacgctatc tattccacac aattcattca ctcgccccat
      241 ccccaatacc gaaccagaaa atagagacaa taaaaccatc aaatatttca tcataaaaaa
      301 cctcactatt ctggcgtacc cgttattttc atcgtctgca acggtccatc cacccgataa
      361 aaaggcagtc cactatgagc tcctctggta cactcaccta aagttttcgc cgcacgcgat
      421 tcaattattg taataaccct tgcctcttct ttattctcat atttcttatc tgttcggctt
      481 aaaagagcca tgaaattttc cttatcaaga aggcaatact gcgcatgagt aaactcatgt
      541 cccaaaccta atgcaggcga ttgtgatttc gagtttaatt cagttgaaca aaataaagat
      601 gctctactat tccaatatat cgtcatatta ttcgaattaa atctaacatt ggttccttca
      661 atatactcta tatttatagt ttcttcagag cttgataaaa aatctatagt ttctttcatt
      721 tgtgaatctt gttttaaata ctcaacagcc tgattaaaat cacttggatc accgttaaca
      781 tggaatttta aacccaaaga atctataaac cgaacagggt ttaatggata tttatacaga
      841 ttccattcac ctctcagccc tatcggatcc tgggtgatat accttccctg caacgggtca
      901 tagtaccggt tgcggttgta gtacagcccc gactcctcat catactgctg ccccggcaga
      961 cggagtgact gctggaggtg ctgcgcgctg gtttcgccca gcaggtttcc ccattcgtca
     1021 tactccccct gccacgccgt ttccccttcc gggctgatga gcgccagcgg cagtccccgg
     1081 tggtcgcagt ggtaaagatg aagtttcctc tccgggatgt attccgcctc cagctgcgcg
     1141 gccatctgct ccgccgtcag cccgcactgc gcaagccatg cctcactctc cgcactcacc
     1201 gctcccgcgc gcagttcccg ctccagcctc cccagcatca ccgacagctc cgccggtagc
     1261 gtcacacccg tgtcctcctg caacacctcc gccaggctcc ggtgccgcgc cttcgcctgt
     1321 tcgccattct ctgtttcgat tctcaggagc ggcgtgaagc tccccggctg gtataccgtc
     1381 tggatacgcg tggtgccggt ctgtatcgtc gtcagcctgt caccgtccca cccgtaccat
     1441 gtcacctccg gtttacgcga cagcgacatc caccccgtca ggtcacgctc ccgccgccat
     1501 acccgttttc ccgtccggcg gcccagcggg tcatagaggt accggctctc cacctgtggt
     1561 tcgccgtgct gtatccgcgt gtagaacacc aggcggtgct ggctgtcgta gtgatagtgg
     1621 tgggtgcgct cgtcgtgcat ccggataacc ccttccggga tacggtccgt cttctccgcc
     1681 agcctgccgt attcatcgta gcgatagaca tagtgcgcat cttccgcgat gcggttatcc
     1741 ggccacgccg tgagggtgct gtccgggtgc agttccgggt ccggcagccg atttcctgcc
     1801 gggtctgttg catacgggat atcgatatcc aggttcgctg ccgtggtgtg gacgcccgtc
     1861 agcctccccg tgtcgctgta acggtactcc cggctctcct gcgggccact gatgcggatt
     1921 agctgtccgt tgtcgttcca gtcgtagtca cggtcaagct gcggaaggtt caggtgcctg
     1981 ctccggagct ggccgctggt attccaggcg gtggcaagtt catatgcctc cccgccgaag
     2041 ctgcgggccg tctcacggtg cagccggtcc cgcgtgtact cgaccagcgg tgttccgccc
     2101 agcttcatcc ccgcaagata accgctgcca tacgtcagcc actccaccgg cggcaggccg
     2161 tccggctcct gacgggtcgc cagcccctgt tcgctgtacg cgtgccccgt ctcatgctcc
     2221 cacagcatct cccccgtctc cggggtctcc accgtctgcc gttcgcccgt cagacggcct
     2281 ttatcgtcat agccgtagtg gaccgccaca cggtggcctt cactcaggtg gctgatttct
     2341 gtcagccagc cgtggtcgtc atactgccac tgctccgccg gttcaccgtt caccgtgcgc
     2401 cgggtgatac ggtccgacgc atcgtaatgc cagagggtga taagcccctc atcttcactc
     2461 tgcgtgagtt ttccggtcag gtcatactgg taacgttgcg tccggccgtc aaagccgcgc
     2521 tgctccgtca gcctgtcgac cgggtcatac cggaacgtgg actggctgcc gttctcgttg
     2581 gtcagcacag tgatgcgccc ggcggcgtca taccccatgc ttcgcgtcag accgccctgc
     2641 gtggtgctga cggcctttcc ccacgcatca tactgtatct cactgcggct gccgtccggg
     2701 gcaacgatag cggtgaggtc tcctgcggcg ctgtactcat aacgggtttc acgcccctgc
     2761 gcatccctct gactgaccag ttgaccgcgc gggttataac tgctgtaagt gctgatgcct
     2821 tcttcccggt gaacggcgat ttgttgaccg taccggtcat actcataccg cgtcgtgtac
     2881 cccgagcagt ccgtaaacgc ctgaagctga ccgtaccggt ttcgcgttat tctccgcctc
     2941 accccggttc cgtcttccac cgcacagggc aggccactgc gggaagaatc ataaaaccag
     3001 cgcgtaatgt taccgttgcg cgagg
//
LOCUS       DAZPAC010000360         3021 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZPAC010000360 DAZPAC010000000
VERSION     DAZPAC010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.367"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            41..718
                     /gene="ospD1"
                     /locus_tag="ACHUPM_004392"
     CDS             41..718
                     /gene="ospD1"
                     /locus_tag="ACHUPM_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HFR0871987.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKSNIINVTNSAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            1042..1152
                     /locus_tag="ACHUPM_004393"
                     /pseudo
     CDS             1042..1152
                     /locus_tag="ACHUPM_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1230..1544
                     /locus_tag="ACHUPM_004394"
     CDS             1230..1544
                     /locus_tag="ACHUPM_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871988.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            1827..2393
                     /gene="ipgB2"
                     /locus_tag="ACHUPM_004395"
     CDS             1827..2393
                     /gene="ipgB2"
                     /locus_tag="ACHUPM_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFR0871989.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSREIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2655..2777)
                     /locus_tag="ACHUPM_004396"
                     /pseudo
     CDS             complement(2655..2777)
                     /locus_tag="ACHUPM_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1008 a          589 c          620 g          804 t
ORIGIN      
        1 aataggttat tgtttttttt agcaaaaagg atgaacaaaa atgtcaataa ataactatgg
       61 attacatcca gcaaacaaca aaaatatgca cctaataata ggcagcaata ctgctaatga
      121 aaataaagga atgaaaagta atatcattaa cgtgacaaat tccgctatat cccacgccat
      181 caatgaagaa aaatcagggg ggggatatag tggtgtttct ttcagaaaat tggccaaaat
      241 acagagcata tccattccga caaagaataa taaggagtat aaccgccata atttgttttc
      301 attgatttgg catggaaatg ccgatgcagc gcgtaaatac ggtgaatcgc tgttggcagc
      361 cgaaataccc aaagaggaaa aactagaagt tcttgcagca cgaaataatg ctggggaatc
      421 tgctttgttc atagctcttc aagaaggtca ttccgctgcg attcaagctt atggagattt
      481 tattaaaact tttgatttat caccaaaaga aacgattaaa ctattggatg taagagataa
      541 tgagggttta ccaggattat ttctggccgc agggaaaggg aatatcgagg ctatgatggc
      601 atatataaat atatgccatc atagtgggat aaaacttaca gaaatagcag acagacttaa
      661 caataatgaa caagacatgt ttaatattat ttctgacaaa atacaagagt tgttttaagt
      721 gtgctaaata gctgcaaaga attgcactta gaaggatctc tccccgaatt gtgtgatctg
      781 cttaatttct aaaaagaaac aaacagttaa ctagactgag cgatactacc catagaacca
      841 gtttcccatg acgaacgaca ctggacgcaa aaacaatcat aaaaaacaca ataaatgtaa
      901 tgaacacatc ccaccctatt aagtaaagtg cactgtgata gaggaaggga aatacaggaa
      961 ggtgtggagc ctcgcagatt tctggcatca tagtgcccaa tacggaatgc ataaagcccc
     1021 ctcagactgg agactccaca aatgaaatat acagtgttta attatatcaa gtgcgattac
     1081 aatcgctgac gacggcacag ttggtgtgga ggtctcagtc ctgaacaatt tgaaaaccag
     1141 aacctcactt aagcctgtgt tcatattacg tgggtaggat catactttta tagaagctgt
     1201 cagcggctac gtcccagcca aatcacttca tgctccagcg ccagagaggc aaggttgggt
     1261 ttgctcagct gccggttgat tttgttgcga ttgaaccgga cagcgtccag ggcgtcggca
     1321 aaagagcgaa tctcaccaat cgttgtttca ttatccgcat taacgatagc ttcaagaaac
     1381 gccaaggttt tatcgaattc atcagcaaca gtggctccgg gcataccgtg accgtttata
     1441 caaaacggcg ttttcagcgt ggagttttca tgaatagcct caacacgaat gtcgtgaagc
     1501 cagtgatgta tcggctacga tcctcagctg atgccagacc ataggactga cactacgaac
     1561 agcaatttta atgacgtaaa tcttcataac tacaactaac agggcatcat tatgaatgat
     1621 gccggttatt ttaccgctgt tggtcccttc atttagggac tgccccaagt tttttcatac
     1681 tctctttctc agagagttta gtctctggga ttccagacgg tccatcattg agccaaggga
     1741 gtaaagaact caaagcattt acagaattgt ttgaatcagc taaaataatg acaatgttac
     1801 tatttttaca tgacaaagtg attataatgc ttggaacatc ttttaataat tttggaatca
     1861 gtttatctca caaaagatat ttttcaggaa aggtggatga gattatccgc tgtacaatgg
     1921 ggaaaaggat agtaaaaata tcatcaacca aaataaatac tagtattttg agctctgttt
     1981 cagaacaaat cggtgaaaat attacagatt ggaaaaatga tgaaaaaaaa gtctacgtat
     2041 cccgggtggt aaaccaatgt attgataagt tttgtgcaga acacagccga gaaatcggcg
     2101 ataacctccg taaacagata tttaaacagg ttgagaaaga ttatcgcatt tctctagaca
     2161 tcaatgctgc acaaagttcc ataaatcatc ttgtatcagg aagcagttat ttcaaaaaga
     2221 aaatggacga actttgtgaa gggatgaata gaagcgttaa aaatgataca acaagcaatg
     2281 ttgcaaatct tatttccgat cagttttttg agaaaaatgt gcaatatatt gatctaaaga
     2341 aattacgtgg gaatatgagt gactatatta caaatttaga atcgcctttc tgatgacaga
     2401 acatcccgag taaagaaact aagaaaagta aatacccagg aattgcctga gcggtgctat
     2461 atttctcctc gcaagtaagt cctaatatat tgaattgttg tatagccagt ttttgtattt
     2521 tcctagctca tggatgtggg atccctctcc cctttctctg ctcaaaggta agcggctcgt
     2581 cagaatcata ttgatattta ctgagaagtc agatcaactt tccaaggcaa cagatcgcgt
     2641 acccggtttg ccggtcagtc ctggatatgt tcaataacgt aacgcagcca tttttctggc
     2701 tccacattgt tcagacggcg tgtgccgatc agcgagaaca acaccgctgc atgttcgtca
     2761 ccgctgtcgg aacctgcgag tcgagtcagc cggagagagt ttcactctcc agctgctcac
     2821 ggaaccgtac gtgacactct cgcgtcatac ggctcttgat cactcattcg atgcacgtag
     2881 taaataccag tgagcaaatt gatcttaccc agcaatagtg gacacgcggc taagtgagta
     2941 aactctcagt cagaggtgac tcacatgaca aaaacagtat caaccagcaa aaaaacacgt
     3001 aaacagcatt cgcctgaatt t
//
LOCUS       DAZPAC010000361         3015 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZPAC010000361 DAZPAC010000000
VERSION     DAZPAC010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.368"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            89..1690
                     /gene="aceB"
                     /locus_tag="ACHUPM_004397"
                     /pseudo
     CDS             89..1690
                     /gene="aceB"
                     /locus_tag="ACHUPM_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            1720..3011
                     /gene="aceA"
                     /locus_tag="ACHUPM_004398"
                     /pseudo
     CDS             1720..3011
                     /gene="aceA"
                     /locus_tag="ACHUPM_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT          754 a          778 c          826 g          657 t
ORIGIN      
        1 gtagtcttag ttgtgctgaa cgaaaagcgc acaacgatcc ttcgttcaca gtggggaagt
       61 tttcggatcc atgacgagga gctgcacgat gactgaacag gcaacaacaa ccgatgaact
      121 ggctttcaca aggccgtatg gcgagcagga gaagcaaatt cttactgccg aagcggtaga
      181 atttctgact gagctggtga cgcattttac gccacaacgc aataaacttc tggcagcgcg
      241 cattcagcag cagcaagata ttgataacgg aacgttgcct gattttattt cggaaacagc
      301 ttccattcgc gatgctgatt ggaaaattcg cgggattcct gcggacttag aagaccgccg
      361 cgtagagata actggcccgg tagagcgcaa gatggtgatc aacgcgctca acgccaatgt
      421 gaaagtcttt atggccgatt tcgaagattc actggccccg gactggaaca aagtgatcga
      481 cgggcaaatt aacctgcgtg atgcagttaa cggcaccatc agctatacca atgaagcagg
      541 caaaatttat cagctcaagc ccaatccagc ggttttgatt tgtcgggtac gcggtctaca
      601 cttgccggaa aaacatgtca cctggcgtgg tgaggcaatc cccggtagcc tgtttgattt
      661 tgcgctctat ttcttccaca actatcaggc actgttggca aagggcagcg gtccctattt
      721 ctatctgccg aaaacccagt cctggcagga agcggcctgg tggagcgaag tcttcagcta
      781 tgcagaagat cgctttaatc tgccgcgcgg cgccatcaag gcgacgttgc tgattgaaac
      841 gctgcccgcc gtgttccaga tggacgaaat ccttcacgcg ctgcgcgacc atattgttgg
      901 tctgaactgc ggtcgctggg attacatctt cagctatatc aaaacgttga aaaactatcc
      961 cgatcgtgtc ctgccagaca gacaggccgt gacgatggat aaaccattcc tgaatgctta
     1021 ctcacgcctg ctgattaaaa cctgccataa acgcggtgct tttgcgatgg gcggcatggc
     1081 agcgtttatt ccgagcaaag atgaagagcg taataaccag gtgctcaaca aagtaaaagc
     1141 ggataaagcg ctggaagtca ataacggtca cgatggcaca tggatcgctc acccaggcct
     1201 tgcggacacg gcaatggcgg tattcaacga cattctcggc tcccgtaaaa atcagcttga
     1261 agtattgcgc gaacaagacg cgccgattac tgccgatcag ctgctggcac cttgtgacgg
     1321 tgaacgcacc gaagaaggta tgcgcgccaa cattcgcgtg gctgtgcagt acatcgaagc
     1381 gtggatctcc ggcaacggct gcgtgccgat ttatggcctg atggaagatg cggcgacggc
     1441 tgaaatttcc cgtacctcga tctggcagtg gatccatcat caaaaaacgt tgagcaatgg
     1501 caaaccggtg accaaagcct tgttccgcta gatgctgggc gaagagatga aagtcattgc
     1561 cagcgaactg ggcgaagaac gtttctccca gggccgtttt gacgatgccg cacgcttgat
     1621 ggaacagatc accacttccg atgagttaat tgatttcctg accctgccag gctaccgcct
     1681 gttagcgtaa accaccacat aactatggag catctgcaca tgaaaacccg tacacaacaa
     1741 attgaagaat tacagaaaga gtggactcaa ccgcgttggg aaggcattac tcgcccatac
     1801 agtgcggaag atgtggtgaa attacgcggt tcagtcaatc ctgaatgcac gctggcgcaa
     1861 ctgggcgcag cgaaaatgtg gcgtctgctg cacggtgagt cgaaaaaagg ctacatcaac
     1921 agcctcggtg cactgactgg cggtcaggcg ctgcaacagg cgaaagcggg tattgaagca
     1981 gtctatctgt cgggatggca ggtagcggcg gacgctaacc tggcggccag catgtatccg
     2041 gatcagtcgc tctatccggc aaactcggtg ccagctgtgg tggagcggat caacaacacc
     2101 ttccgtcgtg ccgatcagat ccaatggtcc gcgggcattg agccgggcga tccgcgctat
     2161 gtcgattact tcctgccgat cgttgccgat gcggaagccg gttttggcgg tgtcctgaat
     2221 gcctttgaac tgatgaaagc gatgattgaa gccggtgcag cggcagttca cttcgaagat
     2281 cagctggcgt cagtgaagaa atgcggtcac atgggcggca aagttttggt gccaactcaa
     2341 gaagctattc agaaactggt cgcggcgcgt ctggcagctg acgtgacggg cgttccaacc
     2401 ctactggttg ctcgtaccga tgccgatgca gcggatctga tcacctccga ttgcgacccg
     2461 tatgacagcg aatttattac cggcgagcgt accagtgaag gcttcttccg tactcatgcg
     2521 ggcattgagc aagcgatcag ccgtggcctg gcgtatgcgc catatgctga cctggtctgg
     2581 tgtgaaacct ccacgccgga tctggaactg gcgcgtcgct ttgcacaagc tatccacgcg
     2641 aaatatccgg gcaaactgct ggcttataac tgctcgccgt cgttcaactg gcagaaaaac
     2701 ctcgacgaca aaactattgc cagcttccag cagcagctgt cggatatggg ctacaagttc
     2761 cagttcatca ccctggcagg tatccacagc atgtggttca acatgtttga cctggcaaac
     2821 gcctatgcct agggcgaggg tatgaagcac tacgttgaga aagtgcagca gccggaattt
     2881 gccgctatac cttcgtatct caccagcagg aagtgggtac aggttacttc gataaagtga
     2941 cgactattat tcagggcggc acgtcttcag tcaccgcgct gaccggctcc actgaagaat
     3001 cgcagttcta agcaa
//
LOCUS       DAZPAC010000362         3010 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZPAC010000362 DAZPAC010000000
VERSION     DAZPAC010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.369"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            63..1226
                     /locus_tag="ACHUPM_004399"
     CDS             63..1226
                     /locus_tag="ACHUPM_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0871990.1"
                     /translation="MPNHDRFECQTAIDSDAILQLAWAVFKIFRIPVNQWTLATAALG
                     GVFLVSGLILLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFK
                     LDPVRYQARVDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKG
                     SQAAVNPFSERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQ
                     LTEAKYNLEQTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQN
                     SLLRLKPGDDAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVL
                     GTIELDPNDDIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1686..2195)
                     /locus_tag="ACHUPM_004400"
     CDS             complement(1686..2195)
                     /locus_tag="ACHUPM_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFR0871991.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNKIKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAI
                     DDSIKCIDE"
     gene            complement(2212..>3010)
                     /locus_tag="ACHUPM_004401"
     CDS             complement(2212..>3010)
                     /locus_tag="ACHUPM_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0871992.1"
                     /translation="QMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYD
                     EWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNN
                     MQCKNNPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLT
                     QSCLNAFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          792 a          649 c          676 g          893 t
ORIGIN      
        1 tcaatgatcg gaagtatgcc aaatcgtgat cgattgtgat gccaaactgt gatcggagat
       61 ggatgccaaa tcatgatcga ttcgaatgcc aaactgcgat cgattcagat gccattttgc
      121 agctggcgtg ggcggtcttt aaaatctttc gtattccggt gaatcagtgg acgctggcga
      181 cggcggcgct gggaggcgtg tttctggtga gtggtttgat tttgttgatg aactacaacc
      241 acccttacac ttttaccgcg caaaaggcag tgatagcgat ccctatcacg ccacaggtga
      301 cgggaattgt tactgaagtc actgacaaga ataatcagct tattcaaaag ggcgaggtgc
      361 tttttaagct cgacccggtt cgttaccagg cgcgagttga cagacttcag gctgacctga
      421 tgacggcgac gcataatata aagacgctgc gtgcgcagct cactgaagcg caggccaaca
      481 ccacccaggt ttcagcggag cgcgaccgtc tgtttaaaaa ttatcaacgt tacctgaaag
      541 gcagccaggc ggcggtgaat ccgttctcgg aacgtgacat cgacgatgcg cggcaaaatt
      601 tcctcgcgca ggatgcgctg gtgaaaggct cggtggcgga gcaggcgcag atccagagcc
      661 agctcgacag tatggttaac ggcgagcaat cgcagattgt gagcttaaga gcgcaactta
      721 ctgaagcaaa atataacctt gagcagactg tcattcgcgc gccgagcaat ggctacgtta
      781 ctcaggtact gatccgccca ggtacatacg cagctgcctt gccgctgcgt ccggtgatgg
      841 tcttcatccc cgagcaaaaa cggcaaattg tcgcccaatt tcggcaaaac tcgctgttac
      901 gtctgaaacc tggcgatgat gcggaagtgg tgtttaacgc gctacctggg caggtgttcc
      961 acggcaaatt gactagtatt ttacctgtcg tgccaggcgg ttcttatcag gcgcaggggg
     1021 tattgcaatc attaacggtc gttcccggca cggacggcgt gctgggaacc attgaactgg
     1081 accctaacga tgatatcgat gccttacccg acggcattta cgcccaggta gcagtttact
     1141 ccgaccattt cagccatgtt tcggtgatgc ggaaggtgct gctaagaatg accagttgga
     1201 tgcattattt gtatttggat cattgagggg gaagggggag attatggaac tttgctccct
     1261 gcaagggcta attatttttg catattactt cattggctgc gatgattttt ctttaatatt
     1321 cattatgtta ataagttctg gatgagtgtg ctctcattgg attttttatc cgtcttgttc
     1381 tgccgcagga tgagactata catcgtcgag atcgcaatga tgatatgagt tgttcctcct
     1441 gcgacggctc taatcaaaaa agagcaggag tgcttttcca tctattgcga tgatggcttt
     1501 attatctgaa ggatgacagt cacatttaaa aaatatttaa tctatagatc acaatgtagg
     1561 gtcatggaat atatgtatac aactattttc ttcttcactt ttaattatcg tattcattat
     1621 aaaactaagt taaaaaaact cctagagagt gagttgttaa tatattttta aataaaatta
     1681 agttatcatt catcgatgca ttttatagaa tcatcaatgg ccataatagt actattaact
     1741 ctcaatttta tatttttatc atcactacaa atattaactg aatttatatc aatgattttt
     1801 ttattcttat cttttatttt atttactgtt tcaatgcaat cagcggcaag ttggagtgga
     1861 tttagatttt tcagatatgg tcttatgatc tttcccttac ttaataaata gcatgtatag
     1921 gattcggaaa gtccagcatc tattttgtac ctcattatat tagataagga tgtgagaaat
     1981 tccttgttat cattcgcagc catatatgcc agtccgagtt cagcctctgc ggccttacag
     2041 gcaaaatagc ttagtgaaca cgcttttcgt ccctctttag tactggctga tattagtgcc
     2101 cataatattg ctttgtctgc caaatctaac tctctctgaa attgtcttgc agtaactgca
     2161 ctcgcagaaa tagagaaaaa aatacaattg ttaataactt tgccatattt tttagtccgt
     2221 aactgttaat gtgctatctg gctctgaaaa tattaactca tttaattttt gcatgtcatt
     2281 agatgaacca gttatacaac cctcggaaca gaatcgaggt cctaaagacg gaccgaaagc
     2341 gtttaaacaa ctttgtgtta aaaatccatc acgattatat gtttctgtat ttgctgacgg
     2401 tactaaacgt ataccattag gttttctatt ggttgctcca ggaccattaa cattccagct
     2461 ccatacccct tgaggtattg gacctctatt ttgtaagtgt gtacaaccag gattattttt
     2521 acattgcata ttattaccat tattaccaga ggcgacagga atagtaagca catcataaga
     2581 gttatcatca ttttgcgtat ctgaaacgca tgaaagcata cttaaagtta tgctgtataa
     2641 acaggtcgct aatcccaacg ggtcaatatc agttatcgga ttcaacggat actgataaaa
     2701 attccatccc cccttcagtc caatcggatc ctgagtgata tatcgcccct gcagcgggtc
     2761 ataatagcgg tggcggttgt aatacaggcc ggactcctca tcatactgct gccccggcag
     2821 gcggataagc tgctgcagct gatgcgggtt ctcttcattc agcaggttgc cccactcatc
     2881 gtattctgcg caccattctg ttgccccttc cgtgctgata agcaccagcg gcaggccgcg
     2941 atggtcgcag tggtacaggt ggatttttcg cgccggcgtg tacaccgggt ccatctggtt
     3001 ttgcatctgc
//
LOCUS       DAZPAC010000363         2993 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZPAC010000363 DAZPAC010000000
VERSION     DAZPAC010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.370"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            347..931
                     /locus_tag="ACHUPM_004402"
     CDS             347..931
                     /locus_tag="ACHUPM_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0871993.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            1036..1776
                     /locus_tag="ACHUPM_004403"
     CDS             1036..1776
                     /locus_tag="ACHUPM_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR0871994.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1811..2993
                     /gene="htrE"
                     /locus_tag="ACHUPM_004404"
                     /pseudo
     CDS             1811..2993
                     /gene="htrE"
                     /locus_tag="ACHUPM_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT          871 a          622 c          616 g          884 t
ORIGIN      
        1 tattttctga aaatcataca tcctctcttt ccttaataaa aattttagtt taaacatttt
       61 tgcagaaaca taatataaat tcccgcagat aaatatcaat cccatcgcca taccctaacc
      121 aattgcactt gtgaatttat ttttgcttaa ttttgaatta agaaaattta atttgttcaa
      181 aagcccttaa agtgcacaat aggcgtccta atgaacaatg gttgtaaatt ttggtaacaa
      241 atatcattgc tcattaatat ctagcatctg cttataacat ttgtgtcatg aatagtcatt
      301 cttcaaacca gtagtctgac taatggaatt ttattcaagg atttttatgt ctaaaaaatt
      361 aggttttgcc ctgtctggtc ttatgctggc aatggttgca ggtactgctt ctgctgatat
      421 ggatggtggt cagttaaata tcagtggtct ggttgttgat aacacctgtg aaacccgcgt
      481 tgacggcggt aataaagatg gtctgatcct gctgcaaact gcaactgttg gtgaaatcga
      541 tgctggcgtg ctgaatgaca ccgttggcgc taaagccaaa cctttcagca tcaccgttga
      601 ctgcagcaaa gcaaatccga atccaggtag tacagctaaa atgaccttcg gttctgtttt
      661 ctttggtaat agcaaaggaa cgctgaacaa cgacatgtct attaacaacc cgtctgacgg
      721 cgtgaatatc gctctgcaca atattgatgg ttccactatc aaacaggttc aaattaacaa
      781 ccctggcgat gtgtatacta aagccctgga tgcaacgaca aaatctgctg tttatgattt
      841 taaagcgtct tacgttcgtg ccgtcgcaga ccaaacagca actgctggtt atgtaaaaac
      901 taacactgca tacaccatta cttatcagta agtattcacc aattaaattg attgaatgaa
      961 tatacaggga atagtgttaa taatttctat ttttatatta ttccctgttt taattaactc
     1021 tgtcagggat ggtttatgtt ttttaacacc aaacatacaa cagctttatg cttcgtaacc
     1081 tgtatggctt ttagttcatc gtctattgcg gacattgtca tttcgggtac tcgcgtaata
     1141 tataaaagcg atcaaaaaag tgtcaacgta cgtctggaaa ataaagggaa taacccgttg
     1201 cttgtccaga gttggttaga tactggcgat aacaacgctg agcctggcag tattacagtc
     1261 ccttttactg ccacgccgcc agtatcgcgt attgatgcca aacgtgggca aacaatcaaa
     1321 ttaatgtaca cagccagcac ctcactgcct aaagacagag agagcgtgtt ctggtttaac
     1381 gtactggaag ttccaccaaa accagatgca gaaaaggtcg cgaatcaaag tctgctgcaa
     1441 ctggcatttc gcacacgtat aaaacttttc tatcgcccgg atggattgaa gggaaatccc
     1501 tctgaagccc cgttagccct gaagtggttc tggtcaggtt cagaaggtaa ggcgtcattg
     1561 cgagtgacca atccaacccc ttactacgtc tcttttagca gtggtgattt agaagctagc
     1621 ggtaaacgct atccgattga tgtgaaaatg attgcaccat ttagtgatga ggtcatgaaa
     1681 gtcactggcc ttaatggcaa agcaagttct gcaaaagtac atttttacgc cattaatgat
     1741 tttggtggcg ccattgaagg taatgccagt ctgtaatcag gcaaggatat aattccgcag
     1801 gaagcataac gtgactataa aaccaactaa aaattatcat aatcatctga cccgtatcgc
     1861 tacgttttgc gcgctgctgt attgcaatag tgctttctgt gctgaacttg ttgaatatga
     1921 ccataccttc ctgatggggc agaatgcctc aaatattgat ctcagccggt atagtgaagg
     1981 taaccctgct ataccgggta tgtacgacgt cagtgtttat gtcaacgatc aacctattat
     2041 taaccaaagc attacattta ttgaaattga aggaaaaaaa acgcacaagc ctgtatcaca
     2101 cttaagaatt tattgcagtt tcatataaat tctcccgata taaataacga aaaagccgtt
     2161 ctgcttgcca gggatgaaac actcggcaat tgccttaatt tgacggaaat tatccctcag
     2221 gcttctgttc gttatgacgt taacgagcaa cgtctggata tagacgttcc tcaggcatgg
     2281 gtaatgaaaa attaccaaaa ctatgttgat ccatcgttat gggaaaatgg cattaatgcg
     2341 gccatgttgt catacaacct caacggatat catagtgaaa ctcctggtcg aagaaatgac
     2401 agcatttatg ctgcttttaa cggtgggatg aatttaggtg catggcgact gcgtgcctca
     2461 ggcaactaca actggatgac cgattctggc agtaattatg attttaagaa tcggtatata
     2521 cagcgtgata tcgcctcgct gcgttctcaa ctcattcttg gtgagtctta tacaacgggc
     2581 gaaacctttg attctgtcag tatccgcggc attcgtttat acagcgacag ccgcatgttg
     2641 cctccgacat tagcgagttt tgcgcctatc attcatggcg ttgctaatac caacgccaaa
     2701 gtcactatta cgcaaggtgg ctataagatt tatgaaacga ctgtgccgcc aggcgctttc
     2761 gtcattgatg atctgagtcc gtcaggttac ggcagcgatc ttattgttac tgtcgaagaa
     2821 tccgatggct caaagcggac attctcgcaa cctttttcat cggtcgttca aatgttacgc
     2881 cctggcgttg gacgttggga tattagcggc ggccaggtct taaaagatga tattcaggat
     2941 gagcctaatt tatttcaggc cagctactac tacggtctga ataactatct aac
//
LOCUS       DAZPAC010000364         2992 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZPAC010000364 DAZPAC010000000
VERSION     DAZPAC010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.371"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1076
                     /gene="bglB"
                     /locus_tag="ACHUPM_004405"
     CDS             <1..1076
                     /gene="bglB"
                     /locus_tag="ACHUPM_004405"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR0871995.1"
                     /translation="RLFDEMAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEH
                     YARTVFTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARA
                     VKACHSLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYM
                     QRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPN
                     PHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIA
                     YLNDHLVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLT
                     RTRKKSFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="ACHUPM_004406"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="ACHUPM_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFR0871996.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="ACHUPM_004407"
     CDS             2805..>2992
                     /locus_tag="ACHUPM_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HFR0871997.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAZPAC010000365         2966 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZPAC010000365 DAZPAC010000000
VERSION     DAZPAC010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.372"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..520)
                     /locus_tag="ACHUPM_004408"
     CDS             complement(<1..520)
                     /locus_tag="ACHUPM_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871998.1"
                     /translation="MFGMSITQSNGNVWMSPEFTPQNLINRGTMSTSKGSVFKTSIPS
                     NKSCFFFIKSSNKANMMFVHEFRDGYNVLRLNQVGGNPGTITVYAFSDMVLPHSGYGI
                     AMYNSAGKMVYHGEMMPLDAEIITITNPQFTINMGYPCAIMPAMVGVYNYRRTPYDRP
                     VYVTMTGATGNQI"
     gene            complement(526..807)
                     /locus_tag="ACHUPM_004409"
     CDS             complement(526..807)
                     /locus_tag="ACHUPM_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0871999.1"
                     /translation="MAYGTGIYNNKGVNVTGFLTPIFFLDRFTTTSGSKTYPNPPPGK
                     SLHAVCSLMPLNNDNHVNIPTPTITINGNTVSWSNLYKGLGSYIYTYWG"
     gene            complement(862..1431)
                     /locus_tag="ACHUPM_004410"
     CDS             complement(862..1431)
                     /locus_tag="ACHUPM_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872000.1"
                     /translation="MCEKCKAIADEQNALFEEMDANELVKMLAILRGIEDVSIFERVV
                     TALNFESTFEEPTQVVALAHHFGVHYLAEKQRADKLQETLDMVIETQGTHDSNKSETI
                     IASKDREIAGLKSSLTMLMSAFNLMSSQAGYKMPSLNSDDPMVVRQLLGAMADQLDDT
                     KSRLEDMMRELSHRHNLATQPHKAFQSSN"
     gene            complement(1571..1729)
                     /locus_tag="ACHUPM_004411"
     CDS             complement(1571..1729)
                     /locus_tag="ACHUPM_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR0872001.1"
                     /translation="MLERFRLVTVIALLVMAVLVDFTGKMMSVISDGVLIGLAIYFAY
                     PLVRKATC"
     gene            complement(1729..2154)
                     /locus_tag="ACHUPM_004412"
     CDS             complement(1729..2154)
                     /locus_tag="ACHUPM_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite/colicin resistance protein"
                     /protein_id="HFR0872002.1"
                     /translation="MLGFFKKKTRKAVIEVKKMENRDAVEATVWGGYMISYANGTCDA
                     KEISILEKTIAALPAFSPFAGEIAQMSANIRAQYEASPRRANAQALRELADVAGTDDA
                     VDVLCLCLDIADQDGIDEPEEQALKKIAQALQLSLDAYL"
     gene            complement(2248..2436)
                     /locus_tag="ACHUPM_004413"
     CDS             complement(2248..2436)
                     /locus_tag="ACHUPM_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872003.1"
                     /translation="MNWFSNHFGKIWLAILALMAAGWVSNIIKLVCSGDLQFQAGMTL
                     ARVVGIFIFPVGSVLGYF"
     gene            complement(2446..>2966)
                     /locus_tag="ACHUPM_004414"
     CDS             complement(2446..>2966)
                     /locus_tag="ACHUPM_004414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872004.1"
                     /translation="FMTLDGVHMARQTYFTSVTKRPRSLRQILAELFSGRVMSRLDEL
                     ETTVRLLNERLDNQASVVANVWAIVASGSSREAKNARPLVKEKNNKDSSNGKFSKKEA
                     ETNGLRSHYSFTGDGSRSSRSEPFDAGFIHHHTSVDDNYHHSSRATCHSGWDDGGCDT
                     SSSSSYSGSCCD"
BASE COUNT          840 a          663 c          607 g          856 t
ORIGIN      
        1 atatttggtt tcccgttgcg cctgtcattg ttacgtatac aggtctgtcg taaggtgttc
       61 gtctatagtt atatactcca accattgcag gcattatcgc acacgggtat cccatattta
      121 tggtaaattg aggatttgta atagtgataa tctctgcatc aaggggcatc atctcaccgt
      181 gatataccat tttacctgcg ctattataca tggcaatacc atatcctgaa tgtggtaaca
      241 ccatatcaga aaaagcataa actgttatcg ttccggggtt tcctccaacc tgattcagtc
      301 tgagaacatt atacccatct ctaaattcat gaacgaacat catattcgcc ttattactgc
      361 tttttataaa gaaaaaacat gatttgttcg acggtattga agtcttaaaa acagaacctt
      421 tagacgtgga cattgtcccc ctattaatca aattttgcgg tgtaaattct ggactcatcc
      481 acacattacc atttgactga gtaattgaca tcccgaacat tattgttatc cccaatatgt
      541 atatatataa gatccaagtc ccttgtataa atttgaccaa cttactgtat tgccgttaat
      601 agtgatagtt ggtgtaggta tatttacatg gttatcgtta tttaaaggca tcaatgagca
      661 cacagcatgt aatgatttgc caggtggtgg atttgggtaa gtcttagagc ctgacgtggt
      721 tgtaaatcga tcaagaaaaa aaataggagt aaggaagccg gtaacgttaa ctcccttatt
      781 attgtagatc cctgtaccgt atgccatttt tcatccttgt ttttatcaac ataatatggc
      841 cgatcatgcg gccatatcag atcaattaga gctttgaaag gctttgtgtg gctgtgttgc
      901 gaggttatgt cgatggctta actcacgcat catgtcttca aggcgactct ttgtgtcgtc
      961 gagttggtca gccattgctc ctaaaagctg acgaactacc atcggatcgt cgctgtttag
     1021 tgatggcatt ttataacccg cctgagaaga catcagattg aatgctgaca tcaacatcgt
     1081 tagagaggat ttaagcccgg caatttcacg atctttgctg gcaataatcg tctcactttt
     1141 gttactgtca tgagttcctt gagtctcgat caccatatcc aaagtctctt gcaacttatc
     1201 agctctctgt ttttcagcaa gataatgaac accgaaatga tgtgctaaag ctacaacctg
     1261 agttggctct tcaaaggtgg actcaaaatt aagtgctgta accactctct caaaaatgga
     1321 aacgtcttct attcctcgaa gaatggctaa cattttgaca agttcattag catccatttc
     1381 ttcgaatagg gcgttttgtt catcagctat tgctttgcat ttctcgcaca tatgttttcc
     1441 ttaatgctta aacaagttgt tttcttattg gctttattgt gatgtggcat aaaaggggag
     1501 caagctaaac gtaaaggtgc aaactctcta tttcggtcaa cgaaacacaa tggccatttg
     1561 acccttgtca ttaacacgtt gctttacgga ctagcggata agcgaagtag atcgccagac
     1621 caatgaggac gccatcagaa atgacagaca tcatctttcc cgtaaaatcc accaacaccg
     1681 ccatcaccag aagagcaatg acggtcacaa gccggaatct ctcaagcatt agagataagc
     1741 atccagtgac aactgaagcg cctgggcgat ctttttcagc gcctgctctt ctggttcatc
     1801 aatgccgtct tggtcggcaa tatcaaggca cagacaaagt acatctactg catcatcagt
     1861 cccggccaca tccgccagct cacgtaaagc ctgggcatta gcacggcgcg gtgaggcttc
     1921 gtattgagcg cggatattgg cgctcatctg ggcaatctca ccagcaaaag gagaaaatgc
     1981 aggcagagct gcaattgttt tctcaagaat ggaaatttct tttgcatcgc atgtaccgtt
     2041 ggcataggag atcatgtacc caccccacac ggtggcttca accgcatcac ggttctccat
     2101 ttttttgact tcgataactg ctttacgagt tttctttttg aagaaaccta acatgtgttt
     2161 tccttttgtt attggtcaaa acaagttgtt ttctagttaa ataaattgct tacgcaaaga
     2221 cggtcaatgc atatgcacta acaaccgtca gaaataacca agtaccgaac cgactggaaa
     2281 aataaaaatc ccaactacac gagccaaggt catgccagcc tgaaactgga gatcgccaga
     2341 gcaaacaagt tttataatgt tcgataccca accggcggcc atgagggcaa gaattgccag
     2401 ccaaattttt ccaaagtgat ttgaaaacca gttcatacaa ccgccttagt cacagcatga
     2461 tcctgagtaa ctggatgaac ttgaggtatc gcatccaccg tcatcccatc cagagtgaca
     2521 ggtcgctcta ctggagtggt ggtaattgtc gtcgacggag gtgtggtgat ggatgaatcc
     2581 ggcatcaaaa ggctctgacc ggctggaacg gctaccgtcg ccagtgaaac tataatgaga
     2641 acgtaggcca ttggtttcag cctctttctt tgaaaatttt ccattcgaac tgtccttgtt
     2701 gtttttctcc ttcactaaag gccgtgcatt tttcgcttcg cgtgaagaac cagaggcaac
     2761 aatcgcccac acgttcgcaa caactgacgc ttgattatct aaacgttcat tcagcaaccg
     2821 aacggtagtc tctagttcat caaggcgtga cataacacga ccgctaaata attcggccaa
     2881 aatttgacgt aaagaacgag gacgtttagt tacagaagtg aaataggttt gacgtgccat
     2941 gtggactcca tccagtgtca taaaga
//
LOCUS       DAZPAC010000366         2966 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZPAC010000366 DAZPAC010000000
VERSION     DAZPAC010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.373"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..216
                     /locus_tag="ACHUPM_004415"
     CDS             <1..216
                     /locus_tag="ACHUPM_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR0872005.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(161..298)
                     /locus_tag="ACHUPM_004416"
     CDS             complement(161..298)
                     /locus_tag="ACHUPM_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFR0872006.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(271..369)
                     /locus_tag="ACHUPM_004417"
                     /pseudo
     CDS             complement(271..369)
                     /locus_tag="ACHUPM_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(358..597)
                     /locus_tag="ACHUPM_004418"
                     /pseudo
     CDS             complement(358..597)
                     /locus_tag="ACHUPM_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            696..1586
                     /locus_tag="ACHUPM_004419"
                     /pseudo
     CDS             696..1586
                     /locus_tag="ACHUPM_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1589..2122
                     /locus_tag="ACHUPM_004420"
     CDS             1589..2122
                     /locus_tag="ACHUPM_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0872007.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2151..2678)
                     /locus_tag="ACHUPM_004421"
     CDS             complement(2151..2678)
                     /locus_tag="ACHUPM_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0872008.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2680..>2966)
                     /locus_tag="ACHUPM_004422"
     CDS             complement(2680..>2966)
                     /locus_tag="ACHUPM_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0872009.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          838 a          661 c          649 g          818 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccag ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg taggccactc
      301 aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact ttttctaggc
      361 ttccaccacc agcacgacaa gatgccgcat acagtgcccc agtcagtcca gttttcagac
      421 aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag gtcggcctgt
      481 ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa caagagcgtt
      541 tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa tcagcacgct
      601 gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgcc attatctaaa
      661 acctcggttt acgagaactc ggcacaagcg gggagaaaat accgttactc agtacagcga
      721 atacctggac taatcgacaa acattcagcg gtggcctttc tggtgaacta tccggcaatg
      781 cttctacagc tgcaaaatta aaaactgcca ggaaaataag caatgtggct tttgatggtt
      841 cctccgatat cacattaaaa gcaagtcatg ttggtgcgtt tgccttaggg aaaacaggaa
      901 gcaccgttgc gaatgataaa gcagttggat ggaactggag tagcggagcc tataacgcaa
      961 ctattagtgg tgcatcaacg ttaattattc atttttatat gggagaagga agttgtcctg
     1021 cagctcagtt tcggattaat tataaaaatg gcggtatttt ttatcgttca gcccgtgatg
     1081 gttatggttt tgaagccgac tggtccgaat tttacaccac cagaaaacct tcagcaggag
     1141 atgttggtgc actgccgtta tctggtggtc aactgaatgg tgcactgggt atcggaacat
     1201 ccagtgctct tggcggtaat tcgattgtat tgggtgataa tgacacgggc tttaaacaaa
     1261 aaggcgatgg taatctggat gtttatgcta ataacgtcca tgttatgcgc tttgtctccg
     1321 gaagcattca aagtaataaa accataaata ttacgggacg tgttaatccc tcggattacg
     1381 gtaactttga ttcccgctat gtgagagatg tcagacttgg cacacgagtt gtttagacca
     1441 tgaagaaagg cgtgatgtat gaaaaatcag gccatgtaat cacggggctt ggtattatcg
     1501 gtgaagtcga tggtgatgat ccggcagtat tcagaccaat acaaaagtta attaacggaa
     1561 catggtataa cgtatcgcag gtataatcat gcagcattta aaaaacattg tcgcaggtaa
     1621 tccaaaaacc gttgaacaat accagctaac aaagaatttt gatgttatct ggttatggtc
     1681 cgaagatgga aaaaattggc atgaggaatt aaaaaacttt caggaggata caataaaact
     1741 ggcttacaca gttgagggaa taattattgc tatagacaag gatgtatcag caattaatcc
     1801 agaaggttta agtgtcgttg agttgcctga tattacagca aatcgccggg ctgatatttc
     1861 gggcaactgg atgttcaaag atggtgcggt gataaagcga acttataccg aggaagagca
     1921 aaggcaacaa gcggaaaatg aaaagcagag cctgctgcag ctcgtcaggg ataaaaccca
     1981 gctgtgggac tctcagctac agctgggcat catttccgat gagaataaac aaaaattaac
     2041 cgagtggatg ctctatgcgc agaaggttga atccacagac acctccagcc tgccagtaac
     2101 gtttcccgaa caaccagaat gagagaaggc ccgctatcgg gccttaattt ttattcaggc
     2161 ttttgtggcc attcaggatt tgccgtatcc acacggctga ccagaacact gtagcgttcc
     2221 catgcttcca gtcgtgtgcg ttcctcgtct gttgccatat tcagcctgac agctcgttcc
     2281 agcggctgga tgactgattc agcttcggaa agcaatgcgg ccttttgtga ttcggcctgt
     2341 tgttgcagtt cctgttctgt atattccctc tttactactt taccatccag aaacatccat
     2401 tttccgttat tgtctgcgtg acgatttgcc gtaatatcag gaacttcaac tatgcttagc
     2461 ccttcaggat taatagttga aacgtcttta ctgacagcaa cgatgatgtt gttctcatcg
     2521 taagcaattt ttatggtgtc tggctgaaaa ttcttttgtt cctcatacca gtttttgcca
     2581 tcttcgcaaa agagccatac cacgcccgct tttttagtta gctggtattg ttccggtgtt
     2641 ttcggattcc cggccttaat atttttcagg tgcatcatct taaacactcc ccacgttgta
     2701 ccatattccc ccaatcaatt tctgaattgg ccttctggca acagtgtcga caatatcatc
     2761 cgcattgctg ttaattgcgg ctgttaaaac ataacctgac gcatcgccaa agccgtagtc
     2821 gcgccatacg tttttatatt caatgctgcc aagacgaata tcccgaacga catgattatg
     2881 cagccatgtg cttaaccagt tgttttccca cactgaacca taaatatcgc cactagacgc
     2941 aacttgcgca ccacttccca gcgcaa
//
LOCUS       DAZPAC010000367         2953 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZPAC010000367 DAZPAC010000000
VERSION     DAZPAC010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.374"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..326
                     /locus_tag="ACHUPM_004423"
     CDS             <1..326
                     /locus_tag="ACHUPM_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0872010.1"
                     /translation="VITMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGAS
                     YRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMK
                     DSQRA"
     gene            428..1882
                     /gene="amn"
                     /locus_tag="ACHUPM_004424"
     CDS             428..1882
                     /gene="amn"
                     /locus_tag="ACHUPM_004424"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR0872011.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2213..2929
                     /locus_tag="ACHUPM_004425"
     CDS             2213..2929
                     /locus_tag="ACHUPM_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0872012.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          802 a          657 c          663 g          831 t
ORIGIN      
        1 gagtcattac catggcgctt gaagcacaat ctattttctg gatagttttc ttctccataa
       61 tgctggcaaa cattgcgcat gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa
      121 tgtttggtgc cagttatcgc tatagtggcg ctggagtcgg ttatcaggtt gccagtgtgg
      181 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      241 ggcatagcgt cgccatttat ttgctggctg gatgcctgat ttccgcaatg accgctttgt
      301 tgatgaaaga cagtcaacgc gcttgatagc ctggcgaaga tcatccgatc ttcgccttac
      361 acttttgttt cacatttctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      421 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      481 cgcgctgtat gagcaatctg tagtcgcatt acgcaacgcc attggcaact atattacaag
      541 tggcgaatta cctgatgaaa acgcccgcaa acaaggtctt tttgtctatc catcactgac
      601 cgtaacctgg gacggtagca caaccaatcc ccccaaaacg cgcgcatttg gtcgttttac
      661 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatcttaa
      721 tgaacaactt acgttgctgt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      781 gcatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      841 gagcgctggg ttaactcgct acttcccgac aacagaactg gcgcaaattg gcgatgaaac
      901 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      961 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagttg aacattttca
     1021 gccgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
     1081 cagccagatc ctcgatcctg atagtcctta cattgccctt tcttgtgctg gcgggaactg
     1141 gatcaccgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1201 ccagatgcca gcatggaatt taattaccgc cgatgctcag ggtattactc tggtgaatat
     1261 tggcgtggga ccatcaaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1321 tgtctggttg atgattggtc actgtggcgg attacgtgaa agtcaggcca ttggcgatta
     1381 tgtacttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1441 tattcctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt
     1501 gagcggcagg cccggtgagg aagtcaaaca gcggctacgt actggtactg tggtaaccac
     1561 agatgacagg aactgggaat tacgttactc agcttctgca cttcgtttta acttaagccg
     1621 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1681 gccatacggg acactactgt gtgtttcaga taaaccatta catggtgaga ttaaacttcc
     1741 cggtcaggct aaccgttttt atgaaggcgc tatttccgaa catctgcaaa ttggcattcg
     1801 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt
     1861 taatgagccg ccgttccgat aataagttct gaaccttctt atcagaaata gatgtgaagg
     1921 agtaagtaag acacctggca aatagcctgc aataatcgtg ggctgtttgc ttccttgggc
     1981 ggatacgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     2041 aaacacaatt ataaaaaatc ccgccaacaa tataagtttt tataaaatta tggctttaga
     2101 atatttttat ttctaataga cgagattttt cctgttatga tataatatgc cgaattaaca
     2161 catgttaacg atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg
     2221 taaatgggcc aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta
     2281 tgcaaaattc ggtgtagaaa tctatgctgc tgctaaacaa ggtgaacccg atccagaatt
     2341 aaacacatct ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt
     2401 tattgataaa gcaattgata aagccaaagg cggcggagat gaaacgttcg tgcagggacg
     2461 ttatgaaggc tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt
     2521 taaccgtacg attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc
     2581 ggcaggttct gtcagctata tgtttgacaa tgctggtgtg attgtattta aaggatcaga
     2641 ccctgaccat atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga
     2701 agaagaaggt aacattgtta tttataccga acctactgac cttcataaag gaatcgcggc
     2761 tctaaaagca gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc
     2821 tgaagttgag ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga
     2881 agatgacgac gatgtacaaa aagtttatca taacgtcgca aatctctaat tatcttttaa
     2941 agaaatctgt ctt
//
LOCUS       DAZPAC010000368         2944 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZPAC010000368 DAZPAC010000000
VERSION     DAZPAC010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.375"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            45..1120
                     /locus_tag="ACHUPM_004426"
                     /pseudo
     CDS             45..1120
                     /locus_tag="ACHUPM_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1136..1884
                     /locus_tag="ACHUPM_004427"
                     /pseudo
     CDS             1136..1884
                     /locus_tag="ACHUPM_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            1886..>2944
                     /locus_tag="ACHUPM_004428"
     CDS             1886..>2944
                     /locus_tag="ACHUPM_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872013.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT          932 a          587 c          613 g          812 t
ORIGIN      
        1 gggcaactgg acgtcaaggt tgaagaagag gacgggccag tctatatatc aatgacacta
       61 accattacat gaattatcag aatgaaaaag agagttatat tgtcacgttt aaccagtatc
      121 ttgaatcatt aaggttaaat acatacgtaa gtttggctcg taatacttac tgggacgcca
      181 gcagtaatgt gaattattca ttatcactta gccgcgattt tgatatcggg ccattaaaaa
      241 acgtctccgc ttctctaaca tttagccgaa taaactggga agaagacaac caggatcaac
      301 tgtacctaaa tatttcgatt ccctggggaa ccagtagaac attgagctat ggtatgcaac
      361 gaaatcagga taacaagatt tcgcatactg cttcgtggta tgactcttcc gatcgaaata
      421 attcctggag cgtttctgct tcaggcgaca atgatgaatt caaagatatg aaagcgtcac
      481 tacgcgccag ttatcagcat aataccgaga acggtcgtct ctatctctcc gggacatcac
      541 agcgagacag ttattattct ctgaatgcca gttggaatgg ttcattcacg gcgactcgtc
      601 acggagccgc tttccatgac tatagtggta gtgctgactc gcgttttatg atcgacgcag
      661 atggtgcaga agatattccg ttgaacaata aacgcgcggt aactaatcgg tatggcatcg
      721 gagttattcc atcagtcagc agttacataa caacatcatt aagcgttgat acccgaaatc
      781 ttccagaaaa tgtggatatc gaaaactcgg ttatcaccac caccttaacc gaaggtgcta
      841 ttggctacgc caaacttgat accccaaggg ctatcaaatc atgggggtta ttcgcctggc
      901 agatggtagt cacccaccac tggggattag cgtaaaagat aaaaccagcc ataaagaatt
      961 aggactggtt gctgatggcg gctttgtata cctcaacggc attcaggatg acagcaaact
     1021 tactttacgc tggggtgata aatcctgttt tattcaacca cccaatagca gcaacttaac
     1081 caccggaacg gttattttac cgtgtattag ccaaaattaa ttcaggatga aactaatgcg
     1141 ctacttaaac acaaagaatc tcattgcagc aggagtatta ctcgcctgca tgagcagcat
     1201 tgcctggggt gccatcatcc cggaccgtac ccgtatcata atgaatgagt cagataaagg
     1261 ggaagcatta aaacttacta atcaaagcaa aaatctaccc tatctggcgc aaacatggat
     1321 agaagatacg aaaggcaata aatcacgtga ctttattgtc acggttcccc ctatggtacg
     1381 tttaaatccc agtgagcaaa ttcaaataag aatgataagt caggaaaaaa ttgctcaact
     1441 gcctaatgac agagaaacgc tcttctattt taacgtgcga gaaataccgc caaaaacaga
     1501 taaaaaaatg tgatgcaagt cactatgcag cacgcattga aactattctg gcgaccaaaa
     1561 gctattgaat tagaagatga tggggtaatg tcttacgaaa aagttgaaat catccgtaga
     1621 aatgatggtt cgatccgctt taacaataag atgccttatc acgttacgct gggttatatc
     1681 ggaacaaacg gaatcacaat gttgccacaa acgcaaagtt taatggtaac cccttatagc
     1741 catgcagata cgcagtttaa aaatgttccg tcagcttttc aggttggtta tatcaatgat
     1801 tttggtggat taagttttta tgaaataaac tgcccgacgg ttaataactc ttgtaatgtt
     1861 tccgtagcca aacgagataa ataatatgcg ctacttatta attgtaatta tatttattat
     1921 aggttttagt gcattacctg tatgggcaat ggactgctat gctgaacatg aagggggaaa
     1981 taccgtagtc attggttacg taccaagaat ttctatcccc agtaatggta aaaaaggtga
     2041 taagatctgg caaagcagtg agtattttat gaatgtcttc tgtaataatg cattacctgc
     2101 cccatcacct ggagaagaat acccatcagc ctggactaat ataatgatgt ttttaccagg
     2161 cggtcaggac ttttataatc agaactccta tatttttggc gtaacataca atggagttga
     2221 ttatgattcg actgccccta atgctatcgc agcaccagaa tgcattgata taaaaggagc
     2281 gggtaccttt aataatcact ataaaaaccc cgctgtctgt agtggaggtc ctgagcctca
     2341 attatcagta acctttccag ctcgcgtaca actctatatt aaattagcaa aaaatgccaa
     2401 tagagttaat aaaaacctgg tgttacctga tgaatatatc gctctagagt ttaaaggtat
     2461 gagtggcgca ggagctatag aagtagacaa aaatttgacc ttcaggattc gtggattaaa
     2521 taatattcat gtccttgact gctttgttaa tgtcgcactg gaaccagctg atggcgttgt
     2581 tgactttggt aaaataaatt cccgaacaat taaaaatacc agcgttagtg agacgtttag
     2641 tgtagtcatg acaaaagatc ctggtgcggc ctgtactgag caattcaata ttttagggag
     2701 ctttttcact acggatattt tgagtgatta tagccatctg gatatgggta atggactgct
     2761 attgaagata tttcataaag atggaacagc aacggaattt aaccgcttct cacaatttgc
     2821 ttctttttca tcgtctagtg caccttcggt caccgcacca ttcagggcag aacttagtgc
     2881 gaacccggca gagactgttg ttgagggacc gtttagtaaa gacgtaatcc tcaaaatcac
     2941 ctat
//
LOCUS       DAZPAC010000369         2940 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZPAC010000369 DAZPAC010000000
VERSION     DAZPAC010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.376"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            266..841
                     /locus_tag="ACHUPM_004429"
     CDS             266..841
                     /locus_tag="ACHUPM_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0872014.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
     gene            1172..2218
                     /gene="mdtG"
                     /locus_tag="ACHUPM_004430"
                     /pseudo
     CDS             1172..2218
                     /gene="mdtG"
                     /locus_tag="ACHUPM_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            2301..2675
                     /locus_tag="ACHUPM_004431"
     CDS             2301..2675
                     /locus_tag="ACHUPM_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFR0872015.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(2676..2903)
                     /locus_tag="ACHUPM_004432"
     CDS             complement(2676..2903)
                     /locus_tag="ACHUPM_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR0872016.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
BASE COUNT          793 a          689 c          696 g          762 t
ORIGIN      
        1 tgaagaatat ctgctcaggt actttccagc cttagatgta tgtgttctta gtagccctgt
       61 ttcagcctta cacctccccc ttgcaaaatc tacgaatatc acttgacaga agccctaaaa
      121 gtatctatgt gacccacgac aagaaacgtc cgtttaatgt ctacatagaa gagtcactat
      181 agctatagac aacaaatcta ccatttgctt taatgtctat agacaagaca aactaaccgt
      241 ttaatgacta cccagaggga tatctatgtc acgaaccttt gcttactgcc gtgtatcagt
      301 agagacttcc accacagaga accagattca agccattaag aaggctggct atgatattcc
      361 taagtcacga gttatagagg aggttgtctc tggttcagtg tcagctatgg atagggacgg
      421 atttgcgaat ctggtcaacc ataagctaga agaaggtgac acccttgttg ttcttaaact
      481 tgaccgtctg ggaagggata acatcgacct acagaataca gtgacccttc ttaatgataa
      541 gggtgtgaag ctcatctgtt tagatttacc cgttaaagac ctttcaagct cagagggtaa
      601 gttggttcta cagctattta gtacctttgc agagtttgaa agaaaccgta tcagagagcg
      661 cactaaacag ggtctagagc gtgctagagt tcaaggtaag gtacttggaa gacctatagc
      721 gagtgacaca actagagcgg tcttagagta caaggaaagg ggactatctc agtctcaggt
      781 agctaaggtt cttaacctct ctgtaaggac tgttagcaga cactggacca agaccctgta
      841 agacccttaa tagactacac agcctccttt atagggggct tttttgtacc tgtgacatag
      901 ctatcaaggc gctatgtaga ggtctttata gcgttgtata ggccagtata cagcactttt
      961 taggaatgga gagggaaacg actagacata acgctaagtt atctattcag actgcacaag
     1021 aatctttatt gatttcaata agttacctta atagtatcta ttcatctcta taccaaatct
     1081 acacagtctt ctaccaataa tactgtataa acatacagca ataacctgta taggctcact
     1141 tatcaggtag tgcattaaga tagcatatat gagcattaca tttttatttt cggccatcgc
     1201 ctcaccgttt tggggtggac tcgccgaccg taaaggccga aaactcatgc tattacgctc
     1261 tgccctcggc atgggcatcg tgatggtgtt gatggggctg gcacaaaata tctggcagtt
     1321 tttgatcctg cgggcgcttc ttgggttact tggcggattt gtccccaacg ctaatgctct
     1381 tatcgccaca caagtaccgc gtaataaaag cggctgggcg ctgggtacgc tctccacagg
     1441 cggcgttagt ggtgcgttgc tcggcccaat ggctggcggc ctgctcgccg atagctacgg
     1501 cttacgtccg gtattcttta ttaccgccag tgtgctcata ctctgctttt tcgtcaccct
     1561 gttttgcata agagaaaaat tccagccgat cagcaaaaaa gagatgctgc acatgcggga
     1621 agtggtgaca tcacttaaaa acccgaaact ggtactcagc ctgtttgtca ctacgttaat
     1681 catccaggtg gcgacgggct caattgcccc cattctgacg ctgtatgtcc gcgaactggc
     1741 gggtaacgtc agtaacgtcg cctttatcag tggcatgatc gcctcggtgc caggcgtggc
     1801 ggctctgcta agtgcaccac gactcggcaa acttggcgat cgaatcggac ccgaaaagat
     1861 cctgattacg gcgctgatct tttctgtact gctgttgatc ccaatgtctt acgttcagac
     1921 gccattgcaa cttgggattt tacgtttttt gctcggtgcc gccgatggtg cactactccc
     1981 cgccgtacag acactgttag tttataactc gagcaaccaa atcgccggac gtatcttcag
     2041 ctataaccaa tcgtttcgtg atattggcaa cgttaccgga ccattgatgg gagcagcgat
     2101 ttcagcgaac tacggtttca gagcggtatt tctcgtcacc gctggcgtag tgttattcaa
     2161 cgcagtctat tcatggaaca gtctacgtcg tcgtcgaata ccccaggtat cgaactgatt
     2221 tttcgccttt catacttgca aaagcggaga atcagctatc cttttccctg aaacctcatc
     2281 aactcaaagg gagaatcgtg atgaccatgt acgcaacgct tgaagaagcc attgacgctg
     2341 cacgcgaaga atttcttgca gacaaccccg gcatcgacgc cgaagatgcg aatgtgcaac
     2401 agttcaatgc ccaaaaatac gttttgcagg acggcgacat catgtggcaa gttgagtttt
     2461 ttgccgacga aggggaagaa ggtgaatgtt tacctatgct tagcggtgaa gccgcgcaaa
     2521 gtgtttttga tggcgactat gatgagatag agatccgcca ggagtggcag gaagagaata
     2581 cattacatga atgggacgag ggggaatttc agcttgagcc accgctggat accgaggaag
     2641 gacacgctgc agctgatgag tgggatgaac gttaatcact catacgggcc atgatgagtg
     2701 tcgatcggca gcagtaaagt atcaaaaacc agggagaatg gcagatcaag gattgtgaca
     2761 taacgccaag cggagtcacg cacatcccat tgcacaccgg gataatattg gttgccgtgc
     2821 ccctgccccg gtatagtgcg gctaatgatg ctgccacagc cactcagcaa ggtcaccatg
     2881 atgctcacca caattaaacg catttgatac ctcttccgta aaaaaatacc ggcataacgc
//
LOCUS       DAZPAC010000370         2917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZPAC010000370 DAZPAC010000000
VERSION     DAZPAC010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.377"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..493
                     /locus_tag="ACHUPM_004433"
     CDS             <1..493
                     /locus_tag="ACHUPM_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR0872017.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="ACHUPM_004434"
     CDS             493..1083
                     /locus_tag="ACHUPM_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR0872018.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1832..2110
                     /locus_tag="ACHUPM_004435"
                     /pseudo
     CDS             1832..2110
                     /locus_tag="ACHUPM_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2170..2658
                     /locus_tag="ACHUPM_004436"
     CDS             2170..2658
                     /locus_tag="ACHUPM_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0872019.1"
                     /translation="MTKQYDVTWLFSEDGKNWYEEQKNFASDTIKMVYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSTITDKSSYNAIEWSVSPEG
                     SS"
     gene            2682..>2917
                     /locus_tag="ACHUPM_004437"
     CDS             2682..>2917
                     /locus_tag="ACHUPM_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0872020.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVY"
BASE COUNT          758 a          636 c          831 g          692 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattacaccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctgtgttggc gacattgctg tatacga
//
LOCUS       DAZPAC010000371         2877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZPAC010000371 DAZPAC010000000
VERSION     DAZPAC010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.378"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..953)
                     /gene="curA"
                     /locus_tag="ACHUPM_004438"
     CDS             complement(<1..953)
                     /gene="curA"
                     /locus_tag="ACHUPM_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR0872021.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDG"
     gene            1134..1652
                     /gene="mddA"
                     /locus_tag="ACHUPM_004439"
     CDS             1134..1652
                     /gene="mddA"
                     /locus_tag="ACHUPM_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR0872022.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1649..2098
                     /locus_tag="ACHUPM_004440"
     CDS             1649..2098
                     /locus_tag="ACHUPM_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0872023.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2099..2332)
                     /gene="ydcY"
                     /locus_tag="ACHUPM_004441"
     CDS             complement(2099..2332)
                     /gene="ydcY"
                     /locus_tag="ACHUPM_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR0872024.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="ACHUPM_004442"
     CDS             complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="ACHUPM_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR0872025.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2786..>2877
                     /gene="yncL"
                     /locus_tag="ACHUPM_004443"
     CDS             2786..>2877
                     /gene="yncL"
                     /locus_tag="ACHUPM_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR0872026.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          691 a          738 c          692 g          756 t
ORIGIN      
        1 aaaccatcag taatatcttc gtggtagtgg attttatcct ctttcaccca ttgccccatc
       61 tccttctgaa actcatggat gcggtgacca taatcctgag cgataataaa cccttgcaag
      121 cgaatacgtt ttttcagcac cgttgccatc aacaggggta aacgatccgg accgggtggt
      181 agctctgtag cgttatagct gctcactaag ccacaaacgg gaatgcgcgc agatgtatta
      241 agtaatggta gcactgcatc aaatacctta ccgcccacgt tttcataata gatatcaata
      301 ccttttgggc acgctttcac cagttgttcg gcaaaatcat ccgcgtggtg atcaagacaa
      361 acatcgaagc ccagcacctc gatagcatgg cggcattttt ccgcgccacc ggcaaccccc
      421 accaccctgc aacctttaag tttgccgatt tgccccaccg ttgctcccac aggacctgtc
      481 gccgcagcta ccaccaacgt ttcgccctct ttaggctgac cgatatccag cagccccata
      541 taagcggtaa agcctggcat ccccagcaca cccagcgacc acgatggatt ttgcggatga
      601 tcgccaagtt tcaccagatc atcaccactg gatatgtcat agtcctgcca tccactgtag
      661 cccagcaccc agtcgctagg ctgataatca ggatgattcg actccacaac acggctcacc
      721 gtgccgccga ccatcacgcc gccaatatca acaggcggtg aataagatgg ctcatcgctc
      781 atacgtccac gcatatacgg gtccagggac aaataaactg tgcgcagtaa cacctgtcct
      841 tcacccggtg tggcgacatc atcttcttca agacggaaat tctccggaac cggtgcgcca
      901 tgtggacgcg aggccagaac ccaacgtcga ttacgctgct tttgttgccc cataatgctc
      961 tcctgtgctt ttggcatgaa tccatcaata gtagcaggct cacgggattg gttttgtttg
     1021 cagccgtcac atcccctctg caacacgcaa taatttcatc gaggaatccg ttgcttcggg
     1081 ctgttttcga tagtattgat aacatcatca tcattatttt atcgaggcca gacatgtcca
     1141 tccgttttgc ccgcaaagcc gattgtgctg ccattgcgga aatttataac cacgccgtgt
     1201 tgtatacggc ggctatctgg aatgaccaaa cggttgatgc agataaccgc attgcctggt
     1261 ttgaagcgcg gaccatagca ggttatccgg tgctggtaag tgaagaagat ggcgtagtga
     1321 cgggatatgc ctcgtttggc gactggcgta gtttcgatgg ttttcgccat accgtggaac
     1381 attcggttta tgtccatccc gatcatcagg gcaaaggtct ggggcgtaaa ttgttaagcc
     1441 gattgattga tgaagcgcgg gattgcggga agcatgtcat ggtcgccggg atcgaatcgc
     1501 aaaatcaggc ctcgctgcat ctgcaccatt cgctgggatt tgtcgtcacc gcacaaatgc
     1561 cgcaggtggg cactaaattt gggcgctggc tggatctgac atttatgcag ttgcaactcg
     1621 acgaacgcac tgaaccggac gcgattggat gaatcagtcg ctcacccttg cttttttgat
     1681 tgccgccgga attggtctgg tagtccagaa cacgctgatg gtgcgcatca cccagacctc
     1741 ctctaccatt ctcatcgcca tgttgctgaa ctcactggtg gggattgtgc tctttgtttc
     1801 tattttatgg tttaaacaag gcatggcggg gtttggcgaa ctggtgtcca gcgtgcgctg
     1861 gtggacactt atacccggct tgctgggatc gttttttgtc ttcgccagta tcagcggata
     1921 tcagaatgtg ggagcggcga caactattgc agtactggtc gccagccagc taattggcgg
     1981 tctgatgctg gatatcttcc gcagtcatgg cgtaccacta cgggcgttgt tcggcccaat
     2041 ttgtggcgca attttacttg tggttggcgc ctggttggta gccagacgct cattttaatc
     2101 agaggatggt gccgcctttg gttaactgtt cgtggcgcgc ttccatatct tctttatgct
     2161 tgcgaccgtg atgagcaata gcggtgcgca aacgctgctg ctgggtgtag cgatcttccc
     2221 gacttaactc cgcgtcatcg ctcagggcga ttaataattc gttcatatgg gcaatgacgc
     2281 tctcttcaat cgcggcatcc acgcgcgcga tgacttcatc cagatgcgac atactttctc
     2341 cttaaccact tgtgaaattt atcgattcat ccaaatgata taagtctgcg tggtgccgtc
     2401 aagccagtga gtaagattta aaacagtgaa aacaacagcg ccaccgggaa actcatcggc
     2461 catgttgccc ccaccagaaa ggcgcttaag aaacggatgc gtttcttatc gtgagaaaga
     2521 aaccaggtga taagaaatgc gattgctgcc ataaccgcat aaaaaacaag catatgttga
     2581 tagagagaca tgtacgggcg tccagccaga tgaataaaac gcgcgcaaat atgcgtcatt
     2641 ttttgacgtg catcaagttt tttcaccagt ttgagaaaaa atcatcaaac agcaacttgc
     2701 ccttatccat cttcagaatg agtattattg cggattttct taacccgtac tatacacata
     2761 ggggcaatga taaaaggtgg caaaaatgaa tgtttccagt agaactgtag tactgataaa
     2821 tgtctttgct gctgttggtt tgtttactct tatctctatg agatttggct ggtttat
//
LOCUS       DAZPAC010000372         2781 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZPAC010000372 DAZPAC010000000
VERSION     DAZPAC010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.379"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            151..1317
                     /gene="nhaA"
                     /locus_tag="ACHUPM_004444"
     CDS             151..1317
                     /gene="nhaA"
                     /locus_tag="ACHUPM_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR0872027.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1383..2282
                     /gene="nhaR"
                     /locus_tag="ACHUPM_004445"
     CDS             1383..2282
                     /gene="nhaR"
                     /locus_tag="ACHUPM_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR0872028.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2320..>2781)
                     /locus_tag="ACHUPM_004446"
     CDS             complement(2320..>2781)
                     /locus_tag="ACHUPM_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR0872029.1"
                     /translation="NYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRF
                     DCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQPSNDGTN
                     EIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          661 a          613 c          697 g          810 t
ORIGIN      
        1 ccgtcaaaaa ctcatctcac cgctgatggc gcaaatcctt taattgctcg taaaaaacga
       61 gttattccta cactataatc tgattttaac gatgatttgt gcggggtaaa atagtaaaaa
      121 cgatctattc acctgaaaga gaaataaaaa gtgaaacatc tgcatcgatt ctttagcagt
      181 gatgcctcgg gaggcattat tcttatcatt gccgctatcc tggcgatgat gatggccaac
      241 agcggcgcaa ccagtggatg gtatcacgac tttctggaga cgccggttca gctccgggtt
      301 ggttcactcg aaatcaacaa aaacatgctg ttatggataa atgacgcgct gatggcggta
      361 tttttcctgt tagtcggtct ggaagttaaa cgtgaactga tgcaaggatc gctagccagc
      421 ttacgccagg ccgcatttcc ggttatcgcc gctattggtg ggatgattgt tccggcatta
      481 ctctatctgg cctttaacta tgccgatccg attacccgcg aagggtgggc gatcccggcg
      541 gctactgaca ttgcctttgc acttggtgtg ctggcgctgt tgggaagtcg tgttccgtta
      601 gcactgaaga tctttttgat ggctctggct attatcgacg atcttggggc catcattatc
      661 atcgcattgt tctacactaa tgacttatcg atggcctctc ttggcgtcgc ggctgtagca
      721 attgcggtac tcgcggtatt gaatctgtgt ggtgtacgcc gcacgggcgt ctatattctt
      781 gttggcgtgg tgttgtggac tgcggtgttg aaatcggggg ttcacgcaac cctggcgggg
      841 gtaattgtcg gcttctttat tcctttgaaa gagaagcatg ggcgttctcc agcgaagcga
      901 ctggagcatg tattgcaccc gtgggtggcg tatctgattt taccgctgtt tgcatttgct
      961 aatgctggcg tttcactgca aggcgtcacg ctggatggct tgacctccat tctgccattg
     1021 gggatcatcg ctggcttgct gattggcaaa ccgctgggga ttagtctgtt ctgctggttg
     1081 gcgctgcgtt tgaaactggc gcatctgcct gagggaacga cttatcagca aattatggcg
     1141 gtggggatcc tgtgcggtat cggttttact atgtctatct ttattgccag cctggccttt
     1201 ggtagcgtag atccagaact gattaactgg gcgaaactcg gtatcctggt cggttctatc
     1261 tcttcggcgg taattggata cagctggtta cgcgttcgtt tgcgtccatc agtttgacag
     1321 gacggtttac cggggagcca taaacggctc ccttttcatt gttatcaggg agagaaatga
     1381 gcatgtctca tatcaattac aaccacttgt attacttctg gcatgtctat aaagaaggtt
     1441 ccgtggttgg cgcagcggag gcgctttatt taactccaca aaccattacc ggacagattc
     1501 gagcgctgga agagcgcctg caaggcaaat tatttaaacg caagggacgt ggtctcgaac
     1561 ccagcgagct gggagaactg gtctatcgct atgccgataa aatgttcacc ttaagccagg
     1621 aaatgctgga tattgtgaac tatcgcaaag aatccaattt attgtttgac gttggcgtgg
     1681 ctgatgcact ttccaaacgc ctggtcagta gcgtacttaa cgccgcagtg gtagaaggcg
     1741 agcccattca tcttcgctgc ttcgaatcca cccacgaaat gctgctggag caattaagtc
     1801 agcataaact ggatatgatc atttctgact gtccgataga ctctacgcag caggaaggcc
     1861 tgttctccgt gagaattggc gaatgtggcg tgagtttctg gtgtacaaat ccaccaccag
     1921 aaaaatcgtt cccggcttgt ctggaagaac ggcgactttt gattcctggg cgacgttcaa
     1981 tgttagggcg caaattgctt aactggttta actcccaggg attaaacgta gaaatcctcg
     2041 gcgagtttga tgatgccgcg ttgatgaaag cttttggtgc gatgcacaat gcaatcttcg
     2101 ttgccccaac gctttatgca tatgactttt atgccgataa aactgtcgta gaaattggtc
     2161 gcgtcgagaa tgtgatggaa gagtaccatg ctatttttgc tgagcggatg attcagcacc
     2221 cggcggtaca gcgaatctgc aatacggatt attctgcgct ttttagtcca gcggcgcgtt
     2281 aatcggcacc tcccccaaag ttaaggtggg ggagatagat tagttgtaca taaccacgat
     2341 tttgactcgg ctcattattt gcccgcttga gacattattt ccatatgtac gcgggcgaat
     2401 aaatagagga atctgattac ttccttcatg gggatgctga aaagagtagt aattgctttt
     2461 accatactgg atttcattcg taccatcatt ggagggttga aagcctactt gacccgcacc
     2521 gtcctcactg ttttctaact cattttttaa tatgctggtt cctgactcct gtatcgtcac
     2581 tggttcaaag taatattcaa catcactggg ttcaaaaact aattcacctt gctcgccctg
     2641 gatgcagtca aatcttatat gatgaaactg ataattttca ttattgatac gagacagtac
     2701 aaatcgtcca gtgtcgatgg attgatcttc aaaaccacag gtcattaaat ttacctgtaa
     2761 aatagtattt aaattataat t
//
LOCUS       DAZPAC010000373         2739 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZPAC010000373 DAZPAC010000000
VERSION     DAZPAC010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.380"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="ACHUPM_004447"
     CDS             complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="ACHUPM_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFR0872030.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
     gene            1322..2374
                     /locus_tag="ACHUPM_004448"
     CDS             1322..2374
                     /locus_tag="ACHUPM_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFR0872031.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
BASE COUNT          849 a          592 c          616 g          682 t
ORIGIN      
        1 gccataaaga aaggaaatgc gctcaacgtt ccgattaacg cacctgtgat ataaacccta
       61 cggcgaccaa aacgatcggc aagccaggca aaacagggaa ttgtcaggca gcttaatcca
      121 cctaccagca aaccaatatt aaggaaaagt tcgcgcggta gccccatatt ctgggttgaa
      181 taattaagtg caaaggcagt aacgatgtac atcgtcagca attcgcacag tcgtagcgca
      241 ataatcttca ggaaagcacc gggatgtcgt aacagcgctt cgataaccgg gatgcgtttt
      301 ttcgcggcag cttgataatg ttgctgttgt tcaaattccg cggactcctc catgccattg
      361 cgcacccaca atgctcccag taccagtacg atgctaaaca ggaaaggaat gcgccagccc
      421 cagcttaaaa actgttcgtc agtcgtcatc atactgatca atgaaaccag tccggttgaa
      481 agcagtaaac ctacaccgta gccaacttgt acaccgctac tgtaaaaggc ttttttattt
      541 ttcggtgcac tttcaacgga aagcaacgcc gcgcctcccc attcgccgcc gacggcaaat
      601 ccctgaatga cacgcagtgt caccagtaaa ataggtgccc accacccaat ggtcgagaat
      661 gaaggaagaa taccaatcaa ggctgtcgcg atgcccatca tccagacggt cagcattaac
      721 atgcgcttac gtcccagtcg gtcgccaaag tgaccgaaaa tgacaccgcc gagcggacgg
      781 aaaaggaatc cgacgccaaa ggtagcaaat gcggcgagcg ttcccatcgc cgggcttact
      841 tgcgggaaaa actcgcgatt aaacaccagt gcggcggtga tgccatagag taaaaaatca
      901 taccagtcga cgacggcacc agcgaagctg cctaacgcag ctcgtcgggc gcgacttaac
      961 gaaagcgtcc cttcatcggg acgagtggag atgagcgtgg agtccataat gatcctgttt
     1021 gtactgtctt tttataatta gtatggaaaa ggtaactcac attgccgtcg atctataaac
     1081 gaaccttatg agactacctc gacagagcgc atctgcaaat gattttatgg ctgattagcg
     1141 gcaacatgaa aaattcgatg gaagatgaaa gcgcaggagt tgctggtgtt gtttaaggat
     1201 taagcagagg acccacaggt tagagatatg aaacataaaa gtccgcatat gcacaaaatg
     1261 gcaccctgat gagtctgata gactgcattg cattataacc agtagggagg cgaggttagg
     1321 tatgttttta gcatcattgt tgagacgtat tgcatttagt tactacgatt ataaagctta
     1381 taatttcaat attgaaaaaa cagactttgt tgtcatccat attcccgatc agattggcga
     1441 tgctatggcc atctttcctg ttattcgggc gcttgaattg cataaaatta agcatctttt
     1501 aattgtaacg tcgacaatta atttagaagt ctttaatgcg cttaaacttg aacagactaa
     1561 attaacatta gtcacaatga ctatgcagga tcacgcaaca ttaaaagaaa taaaagattt
     1621 agcaaagaac ataacacagc aatacggtac gccggatctt tgtattgagg ggatgcgtaa
     1681 aaagaacctg aaaacgatgt tatttatcag tcagttgaaa gcaaaaacga attttcaggt
     1741 tgttggtata accatgaatt gctactcccc tttgtgcaag aacgcgtccc gtatggatca
     1801 gaaactccgg gctcccgtac ctatgacatg ggcatttatg atgcgtgagg cgggttttcc
     1861 agcagtcagg ccaatatatg aattgccact aagtgaggat gtactcgatg aggtgcgcga
     1921 ggaaatgcga tcgttaggat cttacattgc gttcaattta gaaggtagct cgcaggaacg
     1981 tacattttca ttatcgattg cagaaaatct aatagcaaaa attcaaagtg aaacagatat
     2041 accaatagtg atcgttcatg gacccaaagg tgaagataaa gccagggcat tagtggattg
     2101 ttataataat gtctaccgtt tatccttacc accctcgatt aaacgttcag cagcaatcat
     2161 aaaagatgct tatatcgcaa taactcctga cacctcaata ttacatatgg caagtgccta
     2221 taatacccct gttgttgcaa tttatgctga ttacaaaacg cgatggcccg caatggccga
     2281 tgtctcggag tcagtcgtcg ttgggcaaaa aattgacaat ataagtctgg atgaattcgc
     2341 aaaggcatta aaaagtgttt tggcgagaat atgagacagt acttatgcat agtaatgccg
     2401 atctgttatg aacggtggtg tcatcaatac tctgaggctg gtcaatcatc acagaaaacc
     2461 agcctttgaa tcaaagcagt actcaccgga tacgatcata gtcacatcga acgcaattct
     2521 ggcggtgccg catttttatt tattcatttt ctgtgagaga agggcaagct ctatagaggg
     2581 aatgtaaaaa agagagctcc gaatggagca ctctttaaga aattataacg gaaaatcaga
     2641 ggtttttata acatgtcgca taagcaaacc caagcttttc aaatacgctt tgatcacctg
     2701 ttgccaggga aactacgtat tgttcgcttg agtttgcgg
//
LOCUS       DAZPAC010000374         2727 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZPAC010000374 DAZPAC010000000
VERSION     DAZPAC010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.381"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(423..680)
                     /locus_tag="ACHUPM_004449"
     CDS             complement(423..680)
                     /locus_tag="ACHUPM_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872032.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="ACHUPM_004450"
     CDS             complement(677..1174)
                     /locus_tag="ACHUPM_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFR0872033.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="ACHUPM_004451"
     CDS             complement(1376..1834)
                     /locus_tag="ACHUPM_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0872034.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="ACHUPM_004452"
     CDS             complement(1831..2328)
                     /locus_tag="ACHUPM_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR0872035.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHUPM_004453"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHUPM_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFR0872036.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          648 a          708 c          620 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatatct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataacc
//
LOCUS       DAZPAC010000375         2721 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZPAC010000375 DAZPAC010000000
VERSION     DAZPAC010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.382"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            30..2546
                     /locus_tag="ACHUPM_004454"
     CDS             30..2546
                     /locus_tag="ACHUPM_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0872037.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            2557..>2721
                     /locus_tag="ACHUPM_004455"
     CDS             2557..>2721
                     /locus_tag="ACHUPM_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0872038.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          778 a          657 c          630 g          656 t
ORIGIN      
        1 caaatataaa aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac
       61 gcacaccgaa acagttggcg ctcatcatca aacgttgttt gccgatggtg ctcactggca
      121 gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg
      181 aaactacaaa aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac
      241 aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa
      301 aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac
      361 tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata
      421 gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc agctgaattt gatttcaatc
      481 atcagcgact taatttgagc attccccaaa ttgcactgta ccgtgatgca agaggttacg
      541 tctccccttc tcgttgggac gatggtatac caacgctgtt taccaactac tcgtttacag
      601 gttctgataa ccgttaccgc cagggcaatc gtagccaacg acagtacctg aatatgcaaa
      661 atggtgctaa ttttggcccc tggcgattac gcaactattc cacatggaca cgcaacgatc
      721 agacatcaag ctggaatacc atcagtagtt atttacaacg tgatatcaag gcattgaagt
      781 ctcagttgct tctgggagaa agcgccacca gcggcagtat tttttccagc tacaccttta
      841 ctggcgttca actcgcttcc gacgataata tgttgccaaa cagccagcgc ggatttgccc
      901 caacggtacg cggtatcgca aacagtagtg caatcgtgac tatcaggcaa aatggttatg
      961 tgatctatca aagcaacgtg ccagcgggtg cctttgaaat taacgatctc tacccctctt
     1021 ccaacagcgg cgatttagaa gtcacgattg aagaaagtga cggtacgcaa cgtcgcttta
     1081 tccagcctta ttcttcatta cccatgatgc agcgacctgg gcatctaaaa tatagcgcga
     1141 ccgctggacg ctatcgcgct gatgcaaaca gtgatagcaa agaacccgaa tttgctgaag
     1201 ccacggcaat atatggtttg aataatactt ttacgctgta tggtggcctg ctcggttctg
     1261 aagattatta tgcgctgggg atcggtatcg gcggcacact tggcgcactg ggcgcgttgt
     1321 cgatggatat caacagagct gacacccaat tcgataacca gcactctttt catggctatc
     1381 aatggcgtac gcagtacatc aaagatatcc cggaaaccaa caccaatatc gctgtcagct
     1441 actatcgcta taccaacgat ggctatttta gttttgatga agccaatacc cgtaattgga
     1501 actataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca atatttgatg
     1561 gggtaagtct gtatgcctcc ggttcgcagc aagactattg gggcaataac gataaaaaca
     1621 ggaatatctc agttggggtt tccggtcagc aatggggaat tggttacagc ctgaattatc
     1681 aatacagtcg ctacacggat caaaataatg accgcgcact ctctttgaat ctcagtattc
     1741 cgttagaacg ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc
     1801 gcccaaccca acatgaaatg cgtcttaatg gctcactgct ggatgatggt cgcctgagct
     1861 atagcctgga acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt
     1921 accgttcacc ttatggcacc ttcagtgccg gatacagcta cggtaatgac agcagccaat
     1981 acaattacgg cgttaccggc ggcgtggtta tccatactca tggcgtgacg ctctcgcaat
     2041 atctgggcaa cgcttttgcg cttatcgatg ctaatggggc ttctggcgtg aggatacaaa
     2101 actatccggg gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt
     2161 accaggaaaa ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgagc
     2221 aaacaacaca gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca
     2281 atatcggtta tcgcgtactt gttatagtca gcgatcgcaa cggtaaaccg ttgccctttg
     2341 gcgctcttgc cagcaacgat gagacggggc aacaaagtat cgtcgatgag ggcggcatac
     2401 tatatctctc tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg
     2461 cagatcaaca atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat
     2521 tacaaggcac ggcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca
     2581 ggacttttgt tgatatccac atcctgcgct tatgcctcct caggagggtg tggagctgac
     2641 agcactagcg gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca
     2701 gataacgtga caggacggga g
//
LOCUS       DAZPAC010000376         2690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZPAC010000376 DAZPAC010000000
VERSION     DAZPAC010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.383"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            44..769
                     /locus_tag="ACHUPM_004456"
     CDS             44..769
                     /locus_tag="ACHUPM_004456"
                     /inference="COORDINATES: protein motif:HMM:NF045398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adhesin"
                     /protein_id="HFR0872039.1"
                     /translation="MKILNSNLITNISSTIRNHLLTSSKNIKTHPSPNMDVTSHRRLF
                     QKDGIGLSQTGKATQLVIMSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDN
                     DFTKGLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIY
                     RKEYATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKV
                     INESGVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /gene="mobA"
                     /locus_tag="ACHUPM_004457"
     CDS             complement(995..1318)
                     /gene="mobA"
                     /locus_tag="ACHUPM_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFR0872040.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="ACHUPM_004458"
     CDS             1604..1798
                     /locus_tag="ACHUPM_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR0872041.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
     gene            2296..2457
                     /locus_tag="ACHUPM_004459"
     CDS             2296..2457
                     /locus_tag="ACHUPM_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0872042.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHELSPLAAATDNRIRGVRL
                     EVMRLLRLN"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcaggga caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttggtagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAZPAC010000377         2683 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZPAC010000377 DAZPAC010000000
VERSION     DAZPAC010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.384"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            38..1957
                     /locus_tag="ACHUPM_004460"
     CDS             38..1957
                     /locus_tag="ACHUPM_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFR0872043.1"
                     /translation="MIDRILELPRIVKRGIIICIDVVMVIFSFWLSYWLRLDEQTAFL
                     SAPMWFAAAILTIFTVFIFIRIGLYRAVLRYVSAKIMLLIPVGILASTLSLVVISYSL
                     SIMLPRTVVGIYFLVLLLLTSGSRLLFRMILNYGVKGSAPVLIYGAGESGRQLLPALM
                     QAKEYFPVAFVDDNPRLHKAVIHGVTVYPSDKLSYLVDRYGIKKILLAMPSVSKSQRQ
                     KVITRLEHLPCEVLSIPGMVDLVEGRAQISNLKKVSIDDLLGRDPVAPDAKLMAENIT
                     GKAVMVTGAGGSIGSELCRQIVRYKPAKLVLFELSEYALYAIEKELSALCDKEVLNVP
                     VIPLLGSVQRQNRLQMVMKSFGIQTVYHAAAYKHVPLVEHNVVEGVRNNVFGTLYCAE
                     SAIESGVETFVLISTDKAVRPTNTMGTTKRLAELVLQALSARQSQTRFCMVRFGNVLG
                     SSGSVVPLFEKQIAQGGPVTLTHRDIIRYFMTIPEASQLVIQAGAMGHGGDVFVLDMG
                     DPVKIYDLAKRMIRLSGLSVRDDKNPDGDIAIEVTGLRPGEKLYEELLIGDSVQGTSH
                     PRIMTANEVMLPWQDLSLLLKELDQACHDFDHERIRSLLLQAPAAFNPTDDICDLVWQ
                     QKKSLLSQASNVIRL"
     gene            2076..2577
                     /gene="aqpZ"
                     /locus_tag="ACHUPM_004461"
                     /pseudo
     CDS             2076..2577
                     /gene="aqpZ"
                     /locus_tag="ACHUPM_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728363.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
BASE COUNT          622 a          554 c          684 g          823 t
ORIGIN      
        1 ttattaagat tttgtcgcga taataaggta gtgtaggatg attgatagaa tattggagct
       61 gccaagaatt gttaagagag gtatcatcat ctgcattgat gtagttatgg tgatattctc
      121 attttggttg tcttattggt tgaggcttga tgagcaaacg gcttttctta gtgcaccgat
      181 gtggtttgct gcagctattc ttaccatatt taccgtgttt atatttatca ggattgggct
      241 ttatcgggca gtcttacggt atgttagtgc aaagataatg ttgctaatac cagttggtat
      301 tctggcctca acgttatctc ttgtcgttat atcatattcg ctatccataa tgttgccgcg
      361 cactgttgtc ggaatttatt ttttggtttt acttttactg acatcaggct ctagattgct
      421 ttttagaatg atacttaact atggagttaa gggtagtgcg cctgttttga tttatggcgc
      481 tggtgaatct ggccgacaat tattgccagc attaatgcag gcaaaagaat attttcctgt
      541 ggcatttgtg gatgataatc ctcgcttgca taaggctgtc attcatggtg taacagttta
      601 tccctcggat aaactgagtt accttgtaga tcgctatggt ataaagaaaa ttcttttggc
      661 gatgccgagc gtcagtaagt cacaaaggca gaaagtgatt actcgtttag agcatctacc
      721 gtgtgaagtt ctctctattc cgggtatggt cgatttagtc gaaggtcgag cacaaatcag
      781 taatctaaaa aaagtatcga ttgatgactt actaggtcgt gatccggttg ctcctgatgc
      841 caaattgatg gccgaaaaca ttactggcaa agccgttatg gtcactgggg cgggaggctc
      901 gatcggctct gagctttgtc gtcaaattgt tcgatataag ccggccaaat tggttctatt
      961 tgaactgtct gaatatgccc tctacgctat tgagaaagag ctctcggcgc tgtgtgacaa
     1021 agaagttttg aatgttccag tgatccctct gttgggctcg gtgcagcgtc agaatcgctt
     1081 acagatggtg atgaagtcct ttggtattca aacggtttat catgcggccg cttataaaca
     1141 tgtgcctctg gttgagcata atgtggtgga aggggtacgt aataacgtgt ttggtacctt
     1201 gtactgcgct gagtcagcga tcgaaagtgg cgttgaaact tttgtgttga tttccaccga
     1261 taaagcggtg cgcccgacca acactatggg gacaactaag cgtctggccg aattggtatt
     1321 gcaggctttg tctgcacggc aaagccaaac tcgcttttgt atggtgcgat ttggtaatgt
     1381 actcggttct tcgggctctg tcgtgccgtt gtttgaaaaa cagattgccc aaggtgggcc
     1441 agttaccttg actcatcgtg acattattcg ctatttcatg acaattccgg aagcatcaca
     1501 gttggtgatt caagcggggg cgatggggca tggcggcgat gtctttgtct tagacatggg
     1561 cgatccggtc aagatttatg acttagccaa acgcatgatc cggttaagtg gcttgagtgt
     1621 acgggatgat aaaaatccag atggcgatat tgccattgaa gttacgggat tacgtccagg
     1681 tgagaaactg tatgaagaat tactgattgg tgattcagtt caaggtacct ctcatccacg
     1741 aattatgacg gccaacgaag tgatgctacc gtggcaggat ctatcgctct tacttaaaga
     1801 gctggatcaa gcttgtcatg actttgatca tgagcgaatt cgcagtttgt tgttacaagc
     1861 accagcggca ttcaatccaa ctgatgatat ttgcgatcta gtttggcagc agaaaaaatc
     1921 gctgttatca caagcgagca atgtcattcg cctgtgattg cttaggttta accttccaca
     1981 ccaattcttc acctctctta caaatccccg ctaggcggta catcgtgacc gcctttaccc
     2041 tgatgcctgc tctttaacaa acaggacatc agtgtatgtt taaacctttt agcgccgaat
     2101 ttttcggcac tttctggctg gttctgggtg gctgtggtag cgccttgatc tctgctgctt
     2161 tcccacagtt aggtataggc tttttgggcg tggcgttggc gtttggtctg acagtagtca
     2221 ccatggctta tgcggtcggg cacatctctg gtgcgcattt taaccccgcg gtgaccttgg
     2281 gtctgtgggc cggtggacgc ttcccagcag cgcgcgtgtt accttacatt atcgctcagg
     2341 ttatcggcgg tattgccgct gcggcagtgc tgtatggtat cgccagcggt aaggctgggt
     2401 ttgatgcgac aaccagcggt tttgcggcta atggttatgg cctccattca cctggcggct
     2461 atgcgttaag cgcctgtatg ctgagcgagt ttgtcctcag tgcgtttttt gtccggagcg
     2521 acagaaaaac gcgctcctgc gggctttgcg ccactggcga ttggtctggt aatcaccccg
     2581 taaattaacc agcgtcaaaa gtagaatttt ctcgtaccat aaacgcagga gattctttat
     2641 gaaaacatca aaatttaccg acagccaaat catggcgatc ctc
//
LOCUS       DAZPAC010000378         2644 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZPAC010000378 DAZPAC010000000
VERSION     DAZPAC010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.385"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            12..107
                     /locus_tag="ACHUPM_004462"
                     /pseudo
     CDS             12..107
                     /locus_tag="ACHUPM_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            403..1398
                     /gene="pgl"
                     /locus_tag="ACHUPM_004463"
     CDS             403..1398
                     /gene="pgl"
                     /locus_tag="ACHUPM_004463"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR0872044.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1439..2392)
                     /locus_tag="ACHUPM_004464"
     CDS             complement(1439..2392)
                     /locus_tag="ACHUPM_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0872045.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
BASE COUNT          673 a          588 c          648 g          735 t
ORIGIN      
        1 ggggatttac catgcttatt ggctatgtac gcgtatcaac aaatgaccag aataccgatc
       61 tacaacgtaa tgcgtttatt tgtgccggaa gtgagttgat tgttgagcga atgggaagtt
      121 ggccagaagt tgttatggca tagtgtttac ggtcgttgat acaggtaaac aaaagcgggg
      181 agagtcatac tccctgaata gtcgcgaaaa tatctgtggc attgtcaaat tcgtgtcggt
      241 gactaatatt ttagtaatat gccattcgaa atggagtgct aacttattga tttaattgat
      301 gtgcgatatt gtattttatc gtgttttgtt gtctgttttt tatccagtaa gtgttgttat
      361 gtgtatgaaa gataagggat ttatcatttt tgaggtgtgt tcatgaagca aacagtttat
      421 atcgccagcc ctgagagcca gcaaattcac gtctggaacc tgaatcatga aggcgcactg
      481 acgctgacac aggttgtcga tgtgccgggg caggtgcagc cgatggtggt cagcccggac
      541 aaacgttatc tctatgttgg tgttcgccct gagtttcgcg tcctggcgta tcgtattgcc
      601 ccggacgatg gcgcactgac ctttgccgca gagtctgcgc tgccgggtag tccgacgcat
      661 atttccaccg atcaccaggg gcagtttgtc tttgtaggtt cttacaatgc gggtaacgtg
      721 agcgtaacgc gtctggaaga tggcctgcca gtgggcgtcg tcgatgtggt cgaggggctg
      781 gacggttgcc attccgccaa tatctcaccg gacaaccgta cgctgtgggt tccggcatta
      841 aagcaggatc gcatttgcct gtttacggtc agcgatgatg gtcatctggt ggcgcaggac
      901 cctgcggaag tgactaccgt tgaaggggcc ggcccgcgtc atatggtatt ccatccaaac
      961 gaacaatatg cgtattgcgt caatgagtta aacagctcag tggatgtctg ggaactgaaa
     1021 gatccacacg gcaatattga atgtgtccag acgctggata tgatgccgga aaacttctcc
     1081 gacacctgtt gggcggctga tattcatatc accccggatg gtcgtcattt atacgcctgc
     1141 gaccgtaccg ccagtctgat taccgttttc agcgtttcgg aagatggcag cgtgttgagt
     1201 aaagaaggct tccagccaac ggaaacccag ccgcgcggct tcaatgttga tcacagcggc
     1261 aagtatctga ttgtcgccgg gcaaaaatct caccacatct cggtatacga aattgttggc
     1321 gagcaggggc tactgcatga aaaaggccgc tatgcggtcg ggcagggacc aatgtgggtg
     1381 gtggttaacg cacactaacc gctgatttac ccggcgcagt ctctcctgcg ccggtgtatt
     1441 aacctatctc ctgtaacgca tgtctctggc gttcgacgat attggtccac aaattgtctt
     1501 tatcgtcagt ccacaaatta atcagcaagg caaaaaagcg ttctgctgcc ggagaaagca
     1561 cggcatcttt gcggcgaata atccccaatg tccgacgtat aacgggttcc accagtggaa
     1621 taccaataat cgaagaatag ggcgcatggg gcattgccag accaggaagc gccgaaatcc
     1681 ccagtcccgc ctctaccagt cctaatgacg tcgaaagatg acgcacttcg taaaaccagt
     1741 ccagcttcca gggcttgtcg gccagctgtt gttctatcaa cagtcggttg ccgctggagg
     1801 agcgtacgcc aatcattttg taacccacta actcctgcca ttctacaagc tgctttttgg
     1861 ccaatggatg gtcacgccga caggccagca cgaacggctc gttgaccagt ggggtaaaat
     1921 caatggatga atttgtgacg ttgttcatgt ttatgccaaa gtctgattcg ttacacagca
     1981 ccgattccat gcaattattc gtgccttgtt ccagaatccg cactttaata ttgggataca
     2041 gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc ggaatacacg
     2101 ctaacgtcac cataccacga tgataagcat tcatatcacg aatattaaaa agcgtttcat
     2161 cgaatttctt tattaattcc cgcgcctccg ggagcaacct ttttcctgct tttgttaacg
     2221 taaccttgcg agttgtacgc tcaaatagtt gaatatgtaa atcttcctcc atctttttta
     2281 ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt agcggcattg ttaaacgaac
     2341 tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc attattactc
     2401 cggaaaatgg aagcgacgat tttgggtggc tggccgttaa aaattttaac tgcatttagc
     2461 caacttaaat taatgaaaaa atgttattaa tcgttgagct aaagtcatta gagatgcttt
     2521 gcccttaatg taaccatatc gcaataagtt atgtttttaa attgagggca ttattatgaa
     2581 aaaaataccc tgggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc
     2641 gatg
//
LOCUS       DAZPAC010000379         2620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZPAC010000379 DAZPAC010000000
VERSION     DAZPAC010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.386"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..529
                     /gene="virA"
                     /locus_tag="ACHUPM_004465"
     CDS             <1..529
                     /gene="virA"
                     /locus_tag="ACHUPM_004465"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR0872046.1"
                     /translation="DSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPA
                     FISALTSAFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVV
                     PKNDTAISASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIP
                     PESRQVSFAIISPDV"
     gene            708..1395
                     /locus_tag="ACHUPM_004466"
                     /pseudo
     CDS             708..1395
                     /locus_tag="ACHUPM_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    988..1104
                     /locus_tag="ACHUPM_004466"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            1397..1763
                     /locus_tag="ACHUPM_004467"
                     /pseudo
     CDS             1397..1763
                     /locus_tag="ACHUPM_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1766..2164
                     /locus_tag="ACHUPM_004468"
                     /pseudo
     CDS             1766..2164
                     /locus_tag="ACHUPM_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
BASE COUNT          713 a          633 c          688 g          586 t
ORIGIN      
        1 tgatagcaaa aaagagcttc aaatgctaag ttacaaccaa gtaaataaaa taatcaattc
       61 aaacttcccc caacaagacc tatgttttca aacagaaaaa cttttgttta cttcactctt
      121 tcaagaccct gcatttatat ctgcactaac atctgcattc tggcaatctc ttcacatcac
      181 gtcttcctct gtggaacaca tatatgccca aattatgtcc gaaaacatag aaaacaggct
      241 taatttcatg cctgaacaac gagttattaa caattgtgga catattataa aaatcaacgc
      301 agttgtccct aaaaacgaca ctgcaatctc tgcctctggg gggagggctt acgaagttag
      361 ctcatcaata ttaccatcgc acataacatg taatggtgtt ggtattaata aaatagaaac
      421 aagctatctc gttcatgctg gaacacttcc aagcagtgaa gggcttcgca atgctatccc
      481 tccagaaagt cgtcaagtat catttgccat catatctcct gatgtttaag ccaatattat
      541 attggctgcc agactgtaaa aaaacgttct tcagaacagc tctataaact attaaaccaa
      601 agctaaaagg atggggacca atagaatctc agcccatcct ttttatatca ttatgaaccg
      661 ccccggaaat cctggagact aaactccctg agaaagaggt aaacaggatg actaaaaata
      721 cacgtttttc ccctgaagtc cgtcaacggg cagttcgtat ggttctggaa agtcagggcg
      781 aatatgactc acaatgggca gcgatttgtt ccattgcccc aaagattggc tgtatactgg
      841 agactctgcg tgtctgggta cgccagcatg agcgggatac cgggggcggt gaggtgggct
      901 caccaccgct gaacgtcagc gtctgaaaga gccggaacgt gaaaatcgtg aactgcgccg
      961 cagtaacaat atccttcgcc tggcttccgc ttattttgca aaggcggagt tcgaccgcct
     1021 ctggaaaaaa taatgccact gctggataag ctgcgtgagc agtacggggt cggatcggta
     1081 tgcagtgaac tgcatattgc cccgtcaacg tattaccact gtcagcaaca gcgacatcat
     1141 catgataaac gcagtgcccg tgcgcagcgc gatgactggc tgaagaaaga gatactgcgc
     1201 gtatacgatg agaatcatca ggtatacgct gtgcgtaaag tctggcatca gttgttacgg
     1261 gaaggtatca gagtggccag atgcactgtg gcacgtctca tggcggttat ggggcttgcc
     1321 ggtgttctcc ggggtaaaaa ggtccacact accgtcagcc ggaaagccgt tgccgcaggc
     1381 gaccgcgtaa accgtcatca aggcaatatg ccgcgtactc cgggtggccc gcagcggctg
     1441 gtatacgtgg tgtcagcggc ggacaaggat aaacacacgt cagcagttcc gtcagcactg
     1501 cgacagcgtt gtccgcaagg cttttaccca gtccagcgtt acggtgcccc acgcctgacg
     1561 gatgaactgt gtgctctggt taccacttta acgtaaaaac cgtggcagcc agtctgcgcc
     1621 gtcaggaact gagcgcaaaa gcctcccaga agttcagccc gatcagttac cgtgcacacg
     1681 gcctgcctgt atcagaaaat ctgctgacgc aggattttta cgccagtggc ccgaaccaga
     1741 agtgggcggg agacatcacg tactattaca gctcaccgac tgcggggaaa cacggggcgc
     1801 cgggttattg aaggtgacct gtccagctac ttcgacacag tacatcaccg gctgctgatg
     1861 aaagccgtct gccgcaggat cagtgatgca cgttttatga gactgctgtg gaaaacgccg
     1921 tgttgatgtt ggtctcttcc gggcggtcag tgaaagtatg ccacagggcg gtgtgatctc
     1981 gccgttgctg tcgaacatca tactgaatga gttcgaccag tatctgaata aacgctacct
     2041 gagtggaaaa gccagaaagg atcgctggta ctggaatcac agtatccagc gaggccgaag
     2101 tactgctgtc aaagaaaact ggcaatggaa acctgccgtt gcttactgtt gctatgccga
     2161 tgactgtgtg ccacgaaggc gcgtcctggg gacgtgagtc cctgtgcagc catgcggcac
     2221 aagagatgag ggaaggcccc tcggtgtcgg cgtgcaggcg caggcaccaa accatccact
     2281 gctgctgcat ttaagagatg tggtggtgag cgagatggga aaaggtcgga cgtgagccga
     2341 tcaggtatgg ttcgaagaga cgaacgaaag tgaaccgctg atgatgtgtc gaaagcatat
     2401 agatgatgtc aaaacaatgg tggagtcgtt cctgtgggat atgtttactg cctgcgcggc
     2461 atccggcata aaagcggcgt gaagtcgatt caggctcttg tggggaacgt ggacctgtca
     2521 ccccgatgtg aagggagaag cgcaagtgga agccccatga gcgccagagt accaatgcgg
     2581 ggtacaggga cggagcacca agcgattgcg cagtaaacat
//
LOCUS       DAZPAC010000380         2608 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZPAC010000380 DAZPAC010000000
VERSION     DAZPAC010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.387"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            111..443
                     /locus_tag="ACHUPM_004469"
     CDS             111..443
                     /locus_tag="ACHUPM_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR0872047.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            590..1951
                     /gene="yegQ"
                     /locus_tag="ACHUPM_004470"
     CDS             590..1951
                     /gene="yegQ"
                     /locus_tag="ACHUPM_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR0872048.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2281..2598)
                     /gene="yegR"
                     /locus_tag="ACHUPM_004471"
     CDS             complement(2281..2598)
                     /gene="yegR"
                     /locus_tag="ACHUPM_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR0872049.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT          682 a          678 c          619 g          629 t
ORIGIN      
        1 tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac
       61 agcaccttta gatgctgttt tcgatacgca atttcaatca aggagtcatt atggctggtt
      121 ggtttgaact cagtaagagc agtgataatc agttccggtt tgtgctaaaa gcgggcaatg
      181 gtgagactat cctcaccagc gagctgtata catcaaaagc ctctgcggaa aagggcatcg
      241 cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagtaacg
      301 gcaaattcta tttcaatctg aaagccgcca atcatcaaat tatcggctcc agccagatgt
      361 acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc
      421 agacggtgaa agacaatacg taattacgat gccgggcgtg gggtgtcggc gtccggcgct
      481 ttaccagatc cccccacagc gctacaatgc ccgcccttaa agtgggggca ctcccctaac
      541 cgcttcatca ggtgaagcgg agctgacctg tcatcagaac gagagaatta tgtttaaacc
      601 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
      661 agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt gtgcgcaaca acgaattcaa
      721 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
      781 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
      841 ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt
      901 gcgtgagcac ttccctgaaa tgccgatcca cctctcggtg caggctaacg ccgtaaactg
      961 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgagct
     1021 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga ttgagatctt
     1081 cgttcacggt gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
     1141 caagcgcgat ccgaaccagg gtacctgcac caacgcctgc cgctgggagt acaacgtcca
     1201 ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga ttccggtgca
     1261 aaatgttgag ccgacgctag gtatcggcgc gccaaccgac aaagtgttta tgatcgaaga
     1321 agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
     1381 gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca
     1441 ttcgctgaaa atcgaaggcc gtactaaatc tttctactat tgcgcacgca ccgcacaggt
     1501 ttatcgtaaa gctatcgatg atgccgctgc gggcaaaccg ttcgatacca gcctgctgga
     1561 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
     1621 cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg
     1681 tgagtttacc ggtgagcgca agggggacct cgcagcggta gcggtgaaaa ataaattctc
     1741 cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaacttta cccttgagca
     1801 catggaaaac gccaaaggtg aagcgatgcc ggtcgcacca ggcgatggtt atactgtgtg
     1861 gctcccggtc ccgcaggatc ttgagctaaa ttacgcgctg ctgatgcgta atttctccgg
     1921 ggaaaccacg cgtaacccac acggtaagtg attaatttcg attatttttc ccggatggaa
     1981 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
     2041 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
     2101 cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt cttccattga
     2161 ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc atcgtgctcg
     2221 cacaggttcc ggctaagcac aaccagaacg cgcatgtttg atgcttacca aaaaatattc
     2281 tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac cttctgtctt
     2341 caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg catcattttt
     2401 tactatattt tctgcaccag atattcttcc cctagctaaa gaagcttcgg cttcggtgta
     2461 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
     2521 acttgtttca tcattatgca cagcacaacc agatataaga aaaatactaa ttaatgatat
     2581 agcagctatc tttttcatct caccttcc
//
LOCUS       DAZPAC010000381         2579 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZPAC010000381 DAZPAC010000000
VERSION     DAZPAC010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.388"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(253..489)
                     /locus_tag="ACHUPM_004472"
     CDS             complement(253..489)
                     /locus_tag="ACHUPM_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872050.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARA
                     QIELQRYADRQRALREDERQQSDFAWSRPRAPRR"
     gene            1189..1302
                     /locus_tag="ACHUPM_004473"
     CDS             1189..1302
                     /locus_tag="ACHUPM_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0872051.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPERHDLSPLAAATGN"
     gene            1557..1712
                     /locus_tag="ACHUPM_004474"
                     /pseudo
     CDS             1557..1712
                     /locus_tag="ACHUPM_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1806..2450
                     /locus_tag="ACHUPM_004475"
     CDS             1806..2450
                     /locus_tag="ACHUPM_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="HFR0872052.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQ
                     FYDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFM
                     NMITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGE
                     FSTFDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
BASE COUNT          648 a          649 c          655 g          627 t
ORIGIN      
        1 aaaaaaaaag ccccctaagg gctttttttt gtacctgacg gtcagaccga ttaaacataa
       61 catcccttgc ccgcaccccc gcaggcgccc tcagcgcaat cctgctgcat cagggccgtt
      121 tccagcacct ttcctcacca gtatcccgga aaaacgcccc tgatgatttc tgcgcgactg
      181 catggctatg cacgcaggtg aatggcaggt gatttcgcag cgtcagcacc actaaaccgc
      241 caaaagccaa aatcagcggc gcggcgcgcg cggacgtgac caggcaaaat cggactgctg
      301 ccgctcatcc tcacgtaatg cacgctggcg gtcggcgtaa cgctgcaact caatttgcgc
      361 acgcgctctg ttcaggacga tgtgccggtt aggctgattt agctttttac ctgacgctct
      421 ttcagctgcg gcaacagcat catcaagaaa ttttttctct gcaggcgtca agtttgtcag
      481 tctggccata acgttctcca gtcagaataa atcagcacag tctgccagac acagtagaaa
      541 cgatgaagcg ccagcgacgg tgaaaggcag tcagaaacgg tgaacgggag cggattttaa
      601 tccggtgccg tgaggcgttg gacaagccgc aggcgcgtca ttagctttta gccggtttcg
      661 gggcgcagcc ctgaaccagt cacgtagcgc tagcggagtg tatactggct gactatgttg
      721 gcacggatgt ggaaatcagt gaagtgcttc atgtagcagg agaaaaaagg cggcaccagc
      781 gcgtcagcag gatatgtagt acaggatata ttccgcttcc tcgctcactg actcgctgcg
      841 ctcggtcgtt cggctgcggc gagcggtgcc ggcttacagg cggggcggaa atttcctggg
      901 agataccagg aaggagctta acagggaaga gataaggccg cggcgaagcc gtttttccat
      961 aggctccgcc cccctgacaa acatcatgaa atctgacgct caaatcagtg gtggcgaaac
     1021 ccgatgcggc ttaaagatac caggcgtttc cccctggtgg ctccctcgtg ctcccccgca
     1081 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgttta tctcattcca
     1141 cgcctgacac tcagttccgc gaaggcagtt cgctcctgga tggactgtat gcacgaaccc
     1201 cccgttcagt ccgactgctg cgccttatcc ggtaactatc atcttgagtc caacccggaa
     1261 agacacgact tatcgccact ggcagcagcc actggtaact gatttagagg agttagtctt
     1321 gaagttatgc gcctgttaag gctaaactga aagaacagat ttggttactg cgctcctcgt
     1381 aagccagtta ccacggttca aagagttgct gctctgagaa ccttcgaaaa accgccttgc
     1441 agggcggttt tttcgtttac agagcaagag attacgacgc cacagaaagg atctcaagaa
     1501 gatccttcac cgtaatggaa atctatccaa tccgagcaca taggatacac atcgttataa
     1561 cgctggatct gcgtgaattc cagcagcagc aagagaaaga ctttctacaa accagcttac
     1621 agcaggctaa atttaaccag aaaaaagccg ctgaattact gggtctgact tatcatcagc
     1681 tacgtgcctt gctgaagaaa caccagatct aatacgcatt tgcaggtgtc atactggcgg
     1741 gtttgacgca taacgtcata aggtttacca tggcgtcatt actgtataaa aacacaggca
     1801 tagatatgac tctggcatta gttggcgaaa aaattgacag aaatcgcttc accggtgaga
     1861 aagttgaaaa tagtacattt tttaactgcg atttttcagg tgccgacctg agcggcactg
     1921 aatttatcgg ctgccagttc tatgatcgcg aaagtcagaa agggtgcaat tttagtcgcg
     1981 caatgctgaa agatgccatt ttcaaaagct gtgatttatc aatggcagat ttccgcaacg
     2041 tcagtgcctt gggcattgaa attcgccact gccgcgcaca aggcgcagat ttccgcggtg
     2101 caagctttat gaatatgatc accacgcgca cctggttttg cagcgcatat atcactaata
     2161 ctaatctaag ctacgccaat ttttcgaaag tcgtgttgga aaagtgtgag ctgtgggaaa
     2221 accgctggat ggggactcag gtactgggtg cgacgttcag tggttcagat ctctccggcg
     2281 gcgagttttc gactttcgac tggcgagcag caaacttcac acattgcgat ctgaccaatt
     2341 cggagttagg tgacttagat attcggggtg ttgatttaca aggcgttaag ttagacagct
     2401 accaggcatc gttgctcatg gagcggcttg gcatcgctgt gattggttag ttcagcgttt
     2461 acaccgggag atgcgtttga cgatttactt ttacccgcga tcatcgcgtt tattgagttt
     2521 attatttatc tatgcgattc cgatgaaaaa ttcgcacgaa aatatggcta actcatagc
//
LOCUS       DAZPAC010000382         2547 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZPAC010000382 DAZPAC010000000
VERSION     DAZPAC010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.389"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..979)
                     /locus_tag="ACHUPM_004476"
     CDS             complement(<1..979)
                     /locus_tag="ACHUPM_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR0872053.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIY"
     gene            complement(1005..1697)
                     /gene="rfaY"
                     /locus_tag="ACHUPM_004477"
     CDS             complement(1005..1697)
                     /gene="rfaY"
                     /locus_tag="ACHUPM_004477"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR0872054.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYCRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            complement(1707..>2547)
                     /locus_tag="ACHUPM_004478"
     CDS             complement(1707..>2547)
                     /locus_tag="ACHUPM_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR0872055.1"
                     /translation="FVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPT
                     SQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNV
                     STQAACAKRLNIPEMNGRYFNAGVIYVNIKKWHEANLTPYLLTLLRGETKYGSLKYLD
                     QDALNIAFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWH
                     SWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYK
                     LKK"
BASE COUNT          799 a          502 c          440 g          806 t
ORIGIN      
        1 agtaaataaa agcgttaaac gtatcaccat ttttctttgc atgttgatat ttcacttgat
       61 actgcttttc attcgtggct ggaataaacg ccacatcatt ccagcaagaa gcctgataag
      121 cctcattaaa atacttctgg cagggataat tcgtccagtc gttccagggc ttcgttacgc
      181 caacataatg aatcagctta gtatcatcgg taatggtctt tttataatct tcatcgcttt
      241 tattttttaa ctcataatcg atgccataaa agcaatcata atcgccgcta ataaaaatac
      301 aatgaccaaa gaacagaata tttaaaatat cctgatcaag gcatttatac ttcgtgtgct
      361 gcgggtcact tgccagcata gcaatggctt tattcataaa atcatttttc gcccatgctt
      421 caagctgcag atatactaca ccggcgttga aataattccc tggaagtccc tctatagcca
      481 gacgcttcgc tggtttttcc tgcataaaag gcacatcttt aataacagct gcaaacttat
      541 ccccatcaaa attaatatca agcaatccag ctaatgagcc tttacagatg acatcagcat
      601 cgatatataa cagattttca atagtttcac tcagatactg gaacgcaact aaacggaaga
      661 atgttgcata agaccactgt ttcaggctgg ggaagatatc aataaaggca ctggagactt
      721 tataaattct tacttcaacg ttagcatttt tgctggtaaa agcattaaca cgttgtagat
      781 aatcttcatc aatgtagtcg gtaaagagat gaaacttaac cgcaaggtcc ggattatgca
      841 taacaactga ttgcatagat actgccgcac caaataaaaa gttacggtca ataccgtaag
      901 cgatatttaa cacacgatcg gtgttagcaa tttccccaga gacagcgact tcagtaatac
      961 tctcagctaa taaatccatg ttggttccgt tttgactgtg tggtttacgc tttgcctttt
     1021 aattttttaa taaatttgcg tagtttggtt ctgtagatta ctgaatagta gccataatct
     1081 tttatttcgt tggcaatacc caggtgacgt tccatggcca gacgatcacg agctttacgc
     1141 tctgccgtac agcttttacc ggataggtca attatcctga caccatcttt actaacgata
     1201 aagttacccc ggtgtgggtc gcccgatagc atatttaagg catgcaattt ttccattgaa
     1261 gccttaattt ctgctttaac atcttctgga atgatcggca tatcattgag ctctacgccc
     1321 tcgacatact cgataagcat gataaaaaca ctggcatagt tgaaaatttt acgctcagcc
     1381 agaaaataaa aatcattagg aaaagtaaga cctgcacttc tgacacgatc ggtttcaacg
     1441 atcaaattct gatagtagtc accttttaca aaagatttta aaaaacgttc attccttttc
     1501 gttttcgggg caaagacttt aaaaacatag cgacaatatt tcgtatcaat caatgatact
     1561 ttggtatcat taatattacg taatactttt actgttttaa aattataggc cagtatttca
     1621 tcaaagattt ctttatattt attatcatta tctttgtaat aaaatgagaa gccgcgatag
     1681 cgtatacttg taatcataag agtaatttat ttcttaagct tgtacttaat taatgaagtt
     1741 atgcctttaa tatactcacc atgggcaaac agatgcttat attgtttctg catttctgca
     1801 acagtccgcg cctctttaag aggatatttc ttccagggag attgttcacg cgcgatatta
     1861 aagtatgatg cagacggata accagcccag ctatgccatg gtttagttat ccctgtatag
     1921 tgaatcaata ctgttttatc ggtaatggtt tgctgatact ttcgatgact acgatcatga
     1981 agttcgtttt tcagggtata aatagtatca aaatccttcg cgaggtagat attattcata
     2041 ttaaatgcga tattcaacgc atcctgatct aaatatttaa gagagccata tttagtttcc
     2101 cctcgtaaaa gcgtgagtaa atacggtgtc aaatttgctt catgccattt tttaatattg
     2161 acatagataa cgcctgcatt gaaataacgt ccattcatct cgggaatgtt gaggcgtttt
     2221 gcacatgccg cctgagtact aacattgtca ggaatgaccg cagcaaactc atctttaaat
     2281 ataatttctg tgagaggctt cagacttcct ttacaaacga catcggcatc cagatacagc
     2341 agtgtggaaa tactttcact cagatattca aaggacaata ccctgaagta tgtcgcgtac
     2401 gaccaaaact gtgacgtcgg cagatcagca aagtattttg ggtcaattaa atatacaata
     2461 attgaggtat taaattgttt tgctgtttca ttaaatgact ttaaataatc atcatccaca
     2521 taatcagtga aaacatgaaa gacaaaa
//
LOCUS       DAZPAC010000383         2517 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZPAC010000383 DAZPAC010000000
VERSION     DAZPAC010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.390"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..616)
                     /locus_tag="ACHUPM_004479"
     CDS             complement(<1..616)
                     /locus_tag="ACHUPM_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR0872056.1"
                     /translation="MANENLDIDSKTAEELYEWYLDDKLIINRRYQRKLVWSLEEKTA
                     LISSMTQQYPIPLLLFVSIDNKREILDGMQRLEALMSFIEQRFSLNGKYFNLDAIALT
                     KQLKDSGKLQQKEPVLSRESSTLIARYRFAISEYSSTEDHIDEVFRRINSNGKILSKQ
                     ELRSAGCVSNFSELVRKISTIIRGDTTHSDIMGLNKIHNISICND"
     gene            complement(901..1233)
                     /gene="pemK-mt"
                     /locus_tag="ACHUPM_004480"
     CDS             complement(901..1233)
                     /gene="pemK-mt"
                     /locus_tag="ACHUPM_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HFR0872057.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            complement(1235..1492)
                     /gene="pemI"
                     /locus_tag="ACHUPM_004481"
     CDS             complement(1235..1492)
                     /gene="pemI"
                     /locus_tag="ACHUPM_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFR0872058.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(1585..2238)
                     /locus_tag="ACHUPM_004482"
     CDS             complement(1585..2238)
                     /locus_tag="ACHUPM_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFR0872059.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
BASE COUNT          703 a          633 c          520 g          661 t
ORIGIN      
        1 catcattgca aattgatatg ttatgtattt tattcagtcc cattatatcg ctatgtgtag
       61 tatctcctct tataattgtc gatattttcc ttactaactc tgaaaaatta ctaacgcacc
      121 ccgcacttcg tagttcttgt tttgataaaa ttttaccatt ggaatttatg cgtcgaaaca
      181 cttcgtcaat gtggtcctct gttgaagagt attcagatat agcaaatcta tagcgagcaa
      241 ttaatgtaga actttcgcgg gatagcactg gctctttttg ttgaagttta ccactgtctt
      301 ttagttgttt tgttaatgca atggcatcta aattaaagta tttaccattt aatgaaaatc
      361 gctgctcaat aaaactcatt aaagcttcta gtctttgcat tccatcaagg atttctcttt
      421 tgttgtcaat ggaaacaaac aatagcaaag ggatagggta ttgctgagtc attgatgaaa
      481 tcagtgctgt tttctcttcg agggaccata ctaattttct ttggtatctt cgatttataa
      541 ttaatttatc atccagatac cactcgtata attcttctgc tgtcttggag tcaatatcta
      601 agttttcatt agccattaga tgtatctcga gtatttagtt aagaaactga taggtcagta
      661 tttgcatcaa tgttcattga atttgtatcc gaagtcaatc tgatgtagga acttcaaaac
      721 tgcgcaaaac acgcaaatac gcacaagaaa gtagggctgc gcttggaatt cggaaaataa
      781 accacatcaa aactactttt tagccaaata acgtttggga atcaccagaa tggtgggaca
      841 acagcggttt tagtgcccta aatcgtacgt tttcatccag ttgcccctca aaccccatgt
      901 tcaagtcaga atagtggaca ggcggccaag aacttcgttc atgatagtct ccggaacccg
      961 ttcgagtcgt tttccgcccc gtgctttcat atcaattgtc cggggttgat cgcaacgtac
     1021 aacacctgtg gtacgtatgc caacaccatc caacgacacc gcaaagccgg cagtgcgggc
     1081 aaaattgcct ccgctggtta cgggcacaac aacaggcagg cgggtcacgc gattaaaggc
     1141 cgccggtgtg acaatcagca ccggccgcgt tccctgctgc tcatgacctg cggtaggatc
     1201 aagcgagaca agccagattt cccctctttc catgtcagat ttcctcctga ccagtcgccg
     1261 gtgcatccag ccattctcgt tcttcagctg atatttcagc attcggatca cactgtgcca
     1321 gtagctcagc cagtgaatat tgcgggcgtc tgtacggctc aacaatcagc cggccattat
     1381 caatgaccat gccaacttca ttatctgtgc ccagagacag cgcattcagc agtgccggtg
     1441 ggacggtcag cataactgag ccgccaaccc tcttcagtcg ggtggtatgc attcttcacc
     1501 tccataaaag ttatatttaa atataacatc cactaaaaaa acacaccagg ctttaacgca
     1561 caatgtttaa taaaaatata actttcaacc aaacagtaaa cccagcgtgg ttgcttccat
     1621 atgcagcaat accggcagca gcaggccacc acttctgatc ctggccactg atgtaatcaa
     1681 ccccaccagg aacagttctg ccagtgtcag caggttctga tactggctgt gcgcggcgac
     1741 gaacaacaac gacgttatca gcgcccccag ccacatcgtc cagcagtacc gtgaacggaa
     1801 gacgttcagc ataatccccc ggaacagcgt ttcctcattc aacggggcaa ggataaagat
     1861 ggtcagcaac gtcaggatca cgtcaggtat ggacttatcg gcaaaaagtt tcgtcataaa
     1921 tggctcagca ggcagagcca gcgccttacc gagcagaaat acaccgacat acaccacggc
     1981 catcgcaccg accagccacg gtacgccaac gttgcgcagc tgaccaacga ccggtagcgg
     2041 agctatccaa cggcggtata ccaggaaaac acacagcagg tacatcagaa cagtaccatg
     2101 actgaagaac aaatagtttt ttcctgatcc ataaagcaga acggcctgct ccatgacaaa
     2161 tctggctccc caactaatgc cccaggcagc cagcataacc agcataaact gcagatattg
     2221 attacgtgtt tgaatcattg catcgcctgt aaatttttaa cttgtcctat ttttgtcatt
     2281 accacgtata tacacatgta taacaattca gatatcgtta ccaggatatg ccgcatcagc
     2341 ggcatggaag gcggcactct gttgtttcat atgatacagg agtaaaaccg ccgaagcccg
     2401 gcgtaagccg gtactgattg atagatttca ccttacccat ccccagccct gccagaccat
     2461 acccgctttc agccatgaga gagcttctgt gcgcggtcgg agtggtcccg acgaggg
//
LOCUS       DAZPAC010000384         2495 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZPAC010000384 DAZPAC010000000
VERSION     DAZPAC010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.391"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(9..761)
                     /gene="tcyN"
                     /locus_tag="ACHUPM_004483"
     CDS             complement(9..761)
                     /gene="tcyN"
                     /locus_tag="ACHUPM_004483"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR0872060.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(758..1426)
                     /gene="tcyL"
                     /locus_tag="ACHUPM_004484"
     CDS             complement(758..1426)
                     /gene="tcyL"
                     /locus_tag="ACHUPM_004484"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR0872061.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1441..2427)
                     /gene="dcyD"
                     /locus_tag="ACHUPM_004485"
     CDS             complement(1441..2427)
                     /gene="dcyD"
                     /locus_tag="ACHUPM_004485"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR0872062.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT          598 a          709 c          621 g          567 t
ORIGIN      
        1 ttttcttatt attgcagtag aaacttctcg aggaactggc gggtgcgagg ctgctgggga
       61 tcagcaaata acgcttttgc tgccccctgc tcgactatcc gcccctggtc cataaaaatc
      121 gcccggtccg caacatcccg ggcaaagctc atttcgtgcg tcacaatcac catcgtgcgc
      181 ttttcctgcg ccagctggcg gattgtgttc aggacttcgc ccaccagctc tggatccagc
      241 gctgacgttg gctcgtcaaa caaaatcacc tcaggacgca ttgccagcgc acgcgcaatc
      301 gctacacgct gctgttgacc gccagacaaa cgacgtggat agctggtttc tttaccggcc
      361 agcccaactt tcgccagcag ctcgcgagcg cgcgccgtgg cctcttcttt cggttcgcct
      421 ttgacgatca ccggcccttc aataatgttt tccagcaccg tacgatgcgg aaacaaatta
      481 aagttctgga agacaaaccc gacgtgctga cgcaactggc ggatcagcga tttttgctga
      541 cttaatgaac gtgcagtatc aatagtgata tcgccaacgg tgatcgttcc cgcttccggt
      601 tgttccagca gatttatgct gcgtagcaac gtggttttgc cggaaccact cggaccgata
      661 attgccacca cttcgccagg ctttacctcg aggtcgatac cgtgcagcac cgtctgaccg
      721 tgaaattttt tcaccaggtt cttaacttca atggcactca ttttggttct ctctcctggc
      781 gattaagttg attctcaaaa tggttctgca acgtcgataa tactgtcgcc atgatccagt
      841 agatgagcga agccgccagg tacatggtga acacctccag cgtgcgcgag gtaatcaact
      901 gcgcctgacg gaacagctcc ggcacctgga ttgtcgcggc cagcgacgta tcctttacca
      961 ggctgataaa gctgttcgac agcggcggca acgccacccg cgccgcctgt ggcaaaatcg
     1021 cgcgacgcat agtctgccat ggcgtcatac caatactggc agctgcttcc cactgacctt
     1081 tatcaataga agaaatggcg gcgcgcagtg tttcggcggc ataggcggca gtgttcagcg
     1141 acagaccaat cattgctgac ggaatgggat ctaattcgat accaaactgc ggcaggccgt
     1201 aatagatcat aaacagttgt gcgatgagcg gtgtaccacg aaaaatagag atataaaaac
     1261 gcgccagcca gcgcacgggc cagattggcg acaggcgcat cagcgcgaga ataaacccga
     1321 gcagtaagcc aaaaaacatg ccgccgatac tgagttgcag cgtataccca gcccctttca
     1381 gcaggaacgg caaggaatca ataaccagtt gtatactttc ttgcatgagc gttcccggag
     1441 ctaaacgtgg ggatgatagg cgaacagcgc aggcgcaccg ccggtatgaa taaacagaat
     1501 cggcccttca tctttgaagc gtttctgact gatacagcca atcagccccg ccatcgcttt
     1561 tccggtatac acaggatcaa gcagaatgcc ttccagccgc gccagcaatt tcactgcttc
     1621 catgccttcg tcgttcggca cgccgtagcc aggtgcaaaa tagtcatccc agagtataat
     1681 ttccgctgat gcggtcagct ccagttcttt cgcaatcgcc tgttgtaggt taaccacttt
     1741 cggcaactga tcggcaacgg aacgcgacac ggtcacgcca atcagttcgc tttcaggcat
     1801 caggtgttcc agcccaacag ccagtccggc gtgagttccg gcactgcccg atgcgactac
     1861 caccgacgaa atattaaccg ccccttcaca ctgttgcgcg atttccagcg cactctccac
     1921 ataacctagc gcgccaagag cattagaacc gccaaccgga atgacatacg ggcgaaagcc
     1981 ttgtgcttcg actcgcgtcg ccagctcttc cagttgggca ttgggatcgg tcagtgcttc
     2041 gcacatttca atctgggtat tgaacagatc cagcaacaag cgattgccgt tggttaaata
     2101 gttttctgcg gttgtgccaa taggattttc cagcagcgcc acacagtgca gaccgagttt
     2161 cgccgcaact gcggcagtct gccgcacatg gttagactgg atcaccccgg cagtaatcag
     2221 cgtatcggca ccttcgcgca gagcatctgc cgcgagaaat tctagcttac gtaatttatt
     2281 gccgcccatt gccatggggg tgacgtcatc ccgtttgatg aaaatttccc gtcctagata
     2341 atcagaaaag cgcggcagat attcgagcgg cgttggcgcg ccgataaact ccaaccgtgg
     2401 aaaacgggtt aaattatgca gtggcataac agcctccgat gtatgttgtt gtgattttct
     2461 tattatgcac gctgaaaacg cgtaaataaa aaagg
//
LOCUS       DAZPAC010000385         2474 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZPAC010000385 DAZPAC010000000
VERSION     DAZPAC010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.392"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(1..715)
                     /locus_tag="ACHUPM_004486"
                     /pseudo
     CDS             complement(1..715)
                     /locus_tag="ACHUPM_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(768..1364)
                     /locus_tag="ACHUPM_004487"
     CDS             complement(768..1364)
                     /locus_tag="ACHUPM_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR0872063.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1391..1993)
                     /gene="yadL"
                     /locus_tag="ACHUPM_004488"
     CDS             complement(1391..1993)
                     /gene="yadL"
                     /locus_tag="ACHUPM_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFR0872064.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2008..>2474)
                     /locus_tag="ACHUPM_004489"
     CDS             complement(2008..>2474)
                     /locus_tag="ACHUPM_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0872065.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          679 a          538 c          496 g          761 t
ORIGIN      
        1 cttggcctgc gccataggct ttaaatgaat acagataatt tgatgagctg gataatttga
       61 tctgttgagt tactgttgga tcaaagtttg tatctgtata gaattcaatt gtaagggtct
      121 gataaatacc acctacagcc cagtatttac gggtgctatt cgcattattg caactccaat
      181 ataacacgtc ttcgcttgga ttataccacg aaaaatattg ttctgcagag tcaccaatgt
      241 atatcccagg tgaactaacg ttagttacgg taccgtaagc tgaccacatg tttgaaatta
      301 ataatgtgta atacaaacca ggaactgatg tattaaataa tttatgggag ccatacattt
      361 tacctgagtc aaccattgca ttttcaatac gcattgcgct accacttgtg ctatcacttc
      421 tgttgcaata aagaaaacca ccaccagaag cggtatctaa ctgactctac tgtaatttta
      481 cacctgtagg tccaagctgc gtcggtttgt agaaaactaa attatttgta cttgttgcat
      541 atctgaatat gacagtagca tcgccattcg gccccgtttc tctccatttt tgatctgacg
      601 gaccaacgcc gtaactattc ccaacaatat atcctacgtt atcatcagtg ccagcactcg
      661 cggtataaca actcaagcac agaattaaaa aaaaatgtct gaagaatttc ttcattatca
      721 ctccaccatc tactgctgta acagtcgcag ttatttgttt tgatctatta ttcgtagtac
      781 acatcaacta atatatgact cattacttgg ccagatatga catccagcgc gggatcgatt
      841 ttttgcattc tggcataaaa tttccatgaa gaattatgca gggcagaagt cattactgga
      901 tacacggaac gagaatttcc tgctgaacta ataacattga atatattggt cgggtcctga
      961 atggaaaaga gaacgacacc gacattttta gccccggttg cgacttcatt ggcaaatatt
     1021 tgccttgaac ctgcagccat gacaccgcta agggctctaa aatcaatatg taactgatta
     1081 agcacatcgc cagttccctg taatgcacag tctgatactg tgacagtgaa ctcttttcca
     1141 cctggttgat agctttcagg agtaacatta tctgaaaaat atcccacccc aacgacgcca
     1201 agatcaatat taccctggtt actgattccg cttttgcaag tgctgttttt tacattggca
     1261 acaaggtcaa tatcctgccc agcgatggct ggataggtaa tgggaaactg tagtgacaga
     1321 aacagaatta ttctcttcat cagcttacgt tgagtgggat gcatatatag ttcctggatc
     1381 tgtcagtctg ttagttatag gttactgtaa atgtggcagg tgcagaaaat ttacccgcag
     1441 tgacattgct tacggtttta tttttttcga cgaccagtcg agcactcatc ggataataaa
     1501 caaccgcgtt ggctgcattg gagatagtga cctcttgaga tgctggtgtc acacaactaa
     1561 attgtgttgc gctccctgtt gccggagttt cggtgctcca gacttcaacg gcgacggcac
     1621 ttgctttatc tgcggctgtg gatccatttg cgagccccgc accgtcatta gcagtacctt
     1681 cgcatgtggc tcgcttggtt aattttattt gtgctttttt attggggata cccgcacagt
     1741 ctttgaattt gagtttgaat gttttaactt tggtcttggc attgatttca gaaatatata
     1801 catcgccaaa gtcaacaaca gaaatgtcct gatcagagtc atttactaat gtagcggtac
     1861 aagtacctat ttctacggta gtaatcacgg tcagcccaat gctgtctgtt gccgcataag
     1921 agggtacgct gaacaatgag gcagccaaaa cgacactgct ggataatcca taacgtaaat
     1981 ttttaaatgt catcttaatt atccttctta ttgatagatg aaattaaacg ttgccagtgc
     2041 acgaaaattc cccggtacgc cttgccctgc atctgtttca cgtagcgcaa cggtcatttc
     2101 aagaccctta tcgggctgca tctcgtctgt gctccagcgt atcttttccg cactgttagg
     2161 tttaaggaaa gtggcatcat cagtagtccg ttttttgaaa cccataccga tattacttgt
     2221 cgtcgaagat gaatcaccag actgcggtat aataagctta ggtgagcttg atgatgcatt
     2281 tccggtcaga gtggtatcaa tccaactgat gccactgctg cacccactag caaccagttt
     2341 aaaatcagcc tgagattctg tcgttttatt cgcgatctta tccagaccca tcttagggat
     2401 tctcaatgtg tagttattat tgccatcatt cgtgacgtta ttaccagtaa gtgtgatgtt
     2461 acaggttgtc gctt
//
LOCUS       DAZPAC010000386         2446 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZPAC010000386 DAZPAC010000000
VERSION     DAZPAC010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.393"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(25..315)
                     /locus_tag="ACHUPM_004490"
     CDS             complement(25..315)
                     /locus_tag="ACHUPM_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR0872066.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHUPM_004491"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHUPM_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR0872067.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="ACHUPM_004492"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="ACHUPM_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR0872068.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHUPM_004493"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHUPM_004493"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2446)
                     /locus_tag="ACHUPM_004494"
     CDS             complement(2171..>2446)
                     /locus_tag="ACHUPM_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR0872069.1"
                     /translation="GRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          667 a          655 c          549 g          575 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat
     2341 cggcatcacc ccacgccata tgcgtttgtg ctgtgacaga ttttgccagc atgactaccg
     2401 gggacaggcg gcccagtgag tcggggccag cattccagat acgacc
//
LOCUS       DAZPAC010000387         2443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZPAC010000387 DAZPAC010000000
VERSION     DAZPAC010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.394"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            119..721
                     /locus_tag="ACHUPM_004495"
     CDS             119..721
                     /locus_tag="ACHUPM_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFR0872070.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NGICEAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(1018..1839)
                     /locus_tag="ACHUPM_004496"
     CDS             complement(1018..1839)
                     /locus_tag="ACHUPM_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR0872071.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(1950..2246)
                     /locus_tag="ACHUPM_004497"
     CDS             complement(1950..2246)
                     /locus_tag="ACHUPM_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872072.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            complement(2270..2410)
                     /locus_tag="ACHUPM_004498"
     CDS             complement(2270..2410)
                     /locus_tag="ACHUPM_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR0872073.1"
                     /translation="MRQAVAVMPLAAKVLKQVMVNTGGNKRQVPAKRQNRGSRNSTDD
                     GG"
BASE COUNT          688 a          565 c          525 g          665 t
ORIGIN      
        1 tgctgtagct gcgttaacga cgctgcttta aaaaaatcag atttaaacaa tataaatcca
       61 caaatacaac tcaatgatat taaagataaa tcagcaaaaa cttgtttttg cgtagtgtgt
      121 ggtgcttttg gtggtgagaa ccaccaacct gttgagcctt tttgtggagt gggttaaatt
      181 atttacggat aaagtcacca gaggtggaaa aatgaaaaaa tggatgttag caatctgcct
      241 gatgtttata aatgggatct gcgaagccgc cgattgcttt gatcttgcag gtcgggatta
      301 caaaatagac ccggatttac tgagagcgat atcatggaaa gaatcccgtt accgggttaa
      361 tgccatcggt attaatccgg taacgggata tggcagcgga ctgatgcagg tagattccca
      421 gcattttaac gaactggccc gctatggaat taagccggaa catctgacaa cagatccctg
      481 tatgaacatt tataccggtg cttattatct ggcaatagcc tttaaaaaat ggggcgtctc
      541 ctgggaggcc gttggtgcat acaatgccgg attcaggaag accgaacgcc agaaccagag
      601 acgtcttgcc tacgcatcag aggtttaccg gatttatacc tggataaaga gcagtaaagg
      661 catccgggtt ccgaccacga agaaatcact ttcccaaatt aacagtgtgc agaaaaatta
      721 atattgtata cattgtcaaa taccggttat gccgcgacag cggcatggaa ggccacacac
      781 aaaaatatca tatgagtgat aatgctgact ggccgaagcc ccgcaaagcg ggatcccatc
      841 ttatatatat ccggtaactg cgcagcctcc tgccagacca tttttcgcgg gcacaatatt
      901 tacagactac cgaaaagcca gttgatgaaa ttgaggcata tcagacgaca cagcaatgcc
      961 gcaggatgat aatataagta aagccccgaa aaatttttcg gggctttact tatattatca
     1021 tgacttccac tccctgaact gttcggcaat catccataat gctttgttga ttcggatatc
     1081 accgtcaatg cctgtaatgg ctcttgttct ggtattttta ccggaggcac ttcgccccga
     1141 taatccacct tttatcatat tctcctgaac ccgctgccag gttgtccaga gatcgttctg
     1201 tttatcctcc caacggcggg gagtaattat ttgttctggc gtcactggcg ttttattttc
     1261 atcttcgtac ctggccatca atgcagctct gccaaataaa tgttgttcgt cactgttaag
     1321 atgtatctct ttcattgtct ccatattttc agtgacttta tcaaagacac cgagcacttc
     1381 atacgctccc tcaataacct gcccgacaat atcaccttta tgtggaacgc ggatttcgcc
     1441 aaaattattc ccacacacca ggccatttgt gcagacaaaa cgaaaaattc ctgggatcat
     1501 ctgataactg gatgaaccat catgtgaatt aagcaggata atttcaggaa cttctttccc
     1561 gttaatatgc ccttccctgc gaagacgtaa catatgttta ctgtattcgc ggcgtcccaa
     1621 atcacgaacc cgactctgac aggcaaagaa tggctggaaa ccttcatcac gtaacttatt
     1681 gatgatattg atggttggga tatatgtata acgctcactc cgggattcat gtttatcacc
     1741 agaaaagaca gatggaacaa actgcattaa ttcgtcatcc gttaacgggc gctcacggcg
     1801 aatagaatta tatcgaccaa aacgggaagc taatcgcata acagtaactc ctttatgaag
     1861 gcataaaatt aataaaagtt ttttcgctta atatcatccc ccctcggtta tcgttaacaa
     1921 aaagacaaca attccgaggg gaaggaccat caaatcaggt cagttcggga ctgaccaacc
     1981 tgccgagtga aaagcagtaa cagcgcgatc aactcatcag cgttttttct gacaagagga
     2041 gtatgattct ccaggctatc cagtaagtca tgcagctcat tattgacctg gtggagcacc
     2101 ttaaagagtg caccagaatc ccggcagctc tgatatgcaa gatttgcctg aacagcacga
     2161 ttctgcatga tcccgatttg tgcgattgcg tctttctgct gatcgcctgt tggctgataa
     2221 gtggtgttaa aagaaaatgc gctcataatt cctcctgtat ctgatgtttt cagcccccat
     2281 catctgttga gtttcgggag ccgcggtttt gacgttttgc ggggacctgc cttttgttcc
     2341 ctccggtatt caccatcacc tgtttgagaa ccttcgccgc aaggggcatc actgccacgg
     2401 cctgacgcac ctgtcctggg cagaacgcaa aattttttac cgg
//
LOCUS       DAZPAC010000388         2438 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZPAC010000388 DAZPAC010000000
VERSION     DAZPAC010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.395"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            222..338
                     /locus_tag="ACHUPM_004499"
                     /pseudo
     CDS             222..338
                     /locus_tag="ACHUPM_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(443..519)
                     /locus_tag="ACHUPM_004500"
     tRNA            complement(443..519)
                     /locus_tag="ACHUPM_004500"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(483..485),aa:Arg,seq:tct)
     gene            complement(533..609)
                     /locus_tag="ACHUPM_004501"
     tRNA            complement(533..609)
                     /locus_tag="ACHUPM_004501"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(573..575),aa:Arg,seq:tcg)
     gene            complement(617..692)
                     /locus_tag="ACHUPM_004502"
     tRNA            complement(617..692)
                     /locus_tag="ACHUPM_004502"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(657..659),aa:Ile,seq:cat)
     gene            complement(733..1791)
                     /locus_tag="ACHUPM_004503"
     CDS             complement(733..1791)
                     /locus_tag="ACHUPM_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR0872074.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1899..2139)
                     /locus_tag="ACHUPM_004504"
                     /pseudo
     CDS             complement(1899..2139)
                     /locus_tag="ACHUPM_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2364..>2438)
                     /locus_tag="ACHUPM_004505"
     CDS             complement(2364..>2438)
                     /locus_tag="ACHUPM_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0872075.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          586 a          698 c          560 g          594 t
ORIGIN      
        1 atagcggtca tgtgatgccg gtttcccggt aactcagcac cggtatctga gtcaacgttt
       61 tctctactgg gtcatttccg atacgccctg cctgctgaca ggctttcatc acatctgaaa
      121 atatagcacc ctgactgata ctgtagtacc taaggttcca gaaactgtga tgtatccggc
      181 acagaaaagc ccctccggag agggctggag agtggcgcta tgtgccattg catgatgccg
      241 ggtgcctccc ggtgaattca gtaccagcac ctgaatccgc gattatccca tatacctact
      301 cgctgattgc ccctccgcac agggggattc accatgccag tttcttttaa caaactcccc
      361 gcaaaacaga caactgtcaa ccgtctgaat tgtgagacat ttaaaaaaaa ggcccgcaaa
      421 agcgagccag ggaaaataag tgtggcgcgt tgtactggat tcgaaccagt gaccgattgc
      481 ttagaaggca attgctctgt ccggctgagc taacaacgca tgatgcagat aatggaccgc
      541 catcggggac ttgaaccccg cgcagccagc ttcgaaggct ggcgctcttt cctgatgagc
      601 taatggcggt atgtgatggt ggcccttgct ggatttgaac cagcgacctg gcgattatga
      661 gtcgctcgct ctcaccactg agctaaaggg ccgggagccg cataataacg acgcgtaatt
      721 aattcttcaa tatcatccgt tctggctgac taaatcctgt acttcccgaa ccgtctgctc
      781 aaaacgttca gtctccagct caacgccaat tgcacgacgc ccgagcgcca gtgccgcttt
      841 cactgtcgaa cccaacccca tgaaaaaatc tgcaatcagg tcacccggac gactgctagc
      901 gctgattatc tgctgcagca tttctgccgg tttttcgcac ggatgtttcc cgggatagaa
      961 ctgcaccggt ttatgcgtcc acacatcggt atacggcacc tgcaccgtca cgccaaaata
     1021 ccgccgcaga tgtttatatt cactcagcag ctccgcatac tgccggttca gtgaggtata
     1081 cgtatccacc agctggtggt ggagcttttc cagttcacca cgccgatgct tctcttctgc
     1141 cacccgggca aacagcgcct gtaatttcag ataatcgctt tcattcggca actgccactg
     1201 accggcactg aaccagtgcg acaccatgtt tttctttcct gtggcatctg caatctgttt
     1261 tgccgttatc cccagggcag ctcgcgcatc acgaaagtaa gaaatcagcg gggccatcac
     1321 atgctgtttc agtgcactgc ccttcgccgc atacccggca tctttcggac gatacggccc
     1381 ctgataatgt tccgcgaaca gaatgcgctc tgtggcgggg aaatacgccc gcaggctttc
     1441 cttgttgcat ccgttccagc gtccggacgg cttcgcccag ataatatggt tcagcacact
     1501 gaagcgttca cgcatcatga tttcgatatc agatgccagg cgatgaccac agaacaggta
     1561 aagacttccg gcaggtttca gcacccgcca gaactgcgcc agacactggt ccagccactt
     1621 caggtaatca tcgtcgccct tccactggtt atcccagccc tcaggcttca ctttaaagta
     1681 cggcgggtcc gtgactatca ggtcaacaga attttcgggt aacgaccgga taaattccag
     1741 gcagtcggcg ttgattaact cacaactgga tatttttaca gtattaagca tggatcatta
     1801 agcctgtctc tgataggctc attctgcttt tgcgcaaagc agtgggcctg aggtttgctt
     1861 gtgaacccaa cgcatgagca gatggctggt gggtgcccct aacacccacc agccgcccat
     1921 ttaccacaaa taaaaaagcc ttcactgcgg aaggcgtctg taacaaccga actgatagtc
     1981 tgccagaccc gccataacca gctgggtcag tattaactgg cagcgttcgc gtgaaaggta
     2041 agtattctgc gctatctccc cgactgtcgc cggttcggta acgcttaatt cattaaacac
     2101 cactctggcg gtttctgtca tatcctgctg ttttagcatg tcttttcctt ttccggttaa
     2161 cgtgacacac caataactct tgtcgaaaaa gccagcaagc tgaaagacag gtattcaccg
     2221 ccaccagcgc gtttactgta ctgacgcgat ttcagtcata aaaaacccgc tcggcggcgg
     2281 gttgtagaaa ctcttctaac gtcaggcata aaacgcccat cgttatgacg aatttaccac
     2341 agattccgga aaaatcaacc ttgttaccta gttacctttt ttaactgccg ctcagcccat
     2401 gcttcttcaa tatcaaaccg ggtcaccagc gcatcata
//
LOCUS       DAZPAC010000389         2436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZPAC010000389 DAZPAC010000000
VERSION     DAZPAC010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.396"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..306)
                     /locus_tag="ACHUPM_004506"
     CDS             complement(<1..306)
                     /locus_tag="ACHUPM_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR0872076.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     "
     gene            complement(345..632)
                     /locus_tag="ACHUPM_004507"
     CDS             complement(345..632)
                     /locus_tag="ACHUPM_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872077.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(882..1037)
                     /gene="hokD"
                     /locus_tag="ACHUPM_004508"
     CDS             complement(882..1037)
                     /gene="hokD"
                     /locus_tag="ACHUPM_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0872078.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1265..1930)
                     /locus_tag="ACHUPM_004509"
     CDS             complement(1265..1930)
                     /locus_tag="ACHUPM_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR0872079.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(2099..>2436)
                     /locus_tag="ACHUPM_004510"
     CDS             complement(2099..>2436)
                     /locus_tag="ACHUPM_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872080.1"
                     /translation="ELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVRVDEKERQ
                     VRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIERNAKARI
                     RRATKSRTE"
BASE COUNT          610 a          628 c          539 g          659 t
ORIGIN      
        1 ttgctctgcg gcttcgctca gagctggcgt atgttcccgg cctactgatt cacacaggta
       61 atcaacgaaa ccggacacca gttttcgttc gcgaggcgtg atcgccccac cgaccggagt
      121 ccagtaatcg aaacccagtt gcaggagttt gaaaaaacgc ttgtggaatg cgtagttaca
      181 cacacgctta aagtctgcgt gtatccactc acctattttg atttgatgca gaaaatcgca
      241 actctccggc gtcgccggga gaagtaatcc ggaagaggtt tgtttgacca gttgtatatg
      301 tgccatcggc tttctccggt ggcacggtgt tacacagcag gagttcaatc ctgctcaaga
      361 ttgtagatga gtttattctt cagcaaaagc agaaaaccag ccttaaaacc aatctctttc
      421 aaaacccgta atgatgtgac aaattcgtcc tcacgcaaaa taaaaccgtc cgtcagaagt
      481 ccattacaaa aataaaataa cacagcaccg ctcttccttt gttgagattg caaacatcta
      541 atgcggcaat ggctgacaat cgctccattc tcaacgcgca cagcatagag gccattttca
      601 ctaaaaattt cacgcaattc ttcgattttc atcttcagaa tccttccaga taaatagctc
      661 tcccctgttc ggggtccatc cctcttctcc ctgcgcgcta cttaagtgag tcgattctat
      721 ctgcgaaggt gcgcgaatca aattcaccgg aaataaacaa caaaaaaccc gccgaaacgc
      781 tgagagatca cagcgatggg cgggttaagt gcgggtgcgt tgaggatgcc tgacacatca
      841 gaggtgggcg gggatgggat cagctccccg cccggtcact cttacttcct ggattcgtag
      901 tctacgaaga cagcgacctc cgtctggccg gttcggattc gtacctcaca gaggtctttc
      961 ctcgttacca gtgccgtcac tatgacggtt aaacagatga cgatcagggc gattaacatc
     1021 gccttttgct gcttcatagc ctgcttctcc ttgcctttcg gcatgtaaga ggctaaccta
     1081 catgtgcaaa gcatgaaatt ggcctcagat taatgttaag cgtcttgccg gacgcgtaat
     1141 gttaactggg gcttttctct atctgccttt tggtgttcat gcctgaagca gatagcctca
     1201 agcacccgca gtcattctac ttacctcgcc aatatgaaat caatcagaaa ggtgccccat
     1261 aaaatcactc cttctcttct ttaccgtagt ggagttgacc aattttgata agagggcgtc
     1321 cctgagattt gcggtgtaga ttggtatcgc gcagagaata cacacagcca caatattcct
     1381 gctgatagaa tttttcgcgc ttgctgattt caatcatacg ggacgagccg ccctgcttgc
     1441 gccagttata atcccagtac accatgcccg gataatgcgc gacggctcgc cgcccacagt
     1501 cgttaacctg ctgcatattt ttccagcgtg aaatgcccag tgaactgctg atcacactga
     1561 aaccattttc agcggcgtac agcgctgtcc gctcaaaacg catgtcaaaa cacatggtgc
     1621 aacggatccc tcgttcgggc tcccattcca ttcctttggc tcgttcaaac cagttgtctg
     1681 tgtcgtaatc agcatcaata aacggcacgc cgtgttgttc agcaaagcga atattctcat
     1741 ccttacgaat taaatactct ttctgaggat gaatgttcgg gttgtagaaa aagatggtgt
     1801 agtcgattcc cgaggcctga agcgcctcca tcacctcacc ggaacatgga gcacagcaag
     1861 agtgcagtag tagtttgttt gccccgtttg ggagctccaa tttgggccgt ttgaaatcag
     1921 caactgtcat aaatatgttt attggggtca taaaaatatc aaagagtgta gcattagaac
     1981 ggggctatcg gaaacagatg tgtgactgct ccccgccctt tcgggtggtc tcctgatgat
     2041 ttgagggtgc agaaatccct ccggttaagg attaaatttt taacagtgct aaatttaatt
     2101 attcagttct ggattttgtc gccctgcgta tccgcgcttt cgcgttacgc tcaatctgta
     2161 tcagcttttc tatatttctc cgcctttcct gttcctcctg gcgcaatagc cttacatcat
     2221 ctgccagtct ggtttctctt tttgccacag agagcatcca gtcaaacggc tccacaaccg
     2281 caccgcagat tttacagcgg acctgacgct ctttttcgtc aacccggaca gaggcgtgat
     2341 ggcaatatgg tctttccgat ggttcataaa gaaaattaac ctgattacgt gggtcatcct
     2401 cttttaccgg aaataaaacg atattgctta actcat
//
LOCUS       DAZPAC010000390         2402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZPAC010000390 DAZPAC010000000
VERSION     DAZPAC010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.398"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            75..164
                     /locus_tag="ACHUPM_004511"
                     /pseudo
     CDS             75..164
                     /locus_tag="ACHUPM_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            375..641
                     /gene="hipB"
                     /locus_tag="ACHUPM_004512"
     CDS             375..641
                     /gene="hipB"
                     /locus_tag="ACHUPM_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFR0872081.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            641..1962
                     /gene="hipA"
                     /locus_tag="ACHUPM_004513"
                     /pseudo
     CDS             641..1962
                     /gene="hipA"
                     /locus_tag="ACHUPM_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2146..2247
                     /locus_tag="ACHUPM_004514"
                     /pseudo
     CDS             2146..2247
                     /locus_tag="ACHUPM_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          666 a          505 c          618 g          613 t
ORIGIN      
        1 agatttggcg cagtggagta gcaacgcgca ggatcgctgg catcttggcg tgatggcagg
       61 ctacgccaat cagcattagc ctgtggggga atgtcggtgt gcaactaggt gataaaggct
      121 atagcgatac tcagggcatg ctgggagtga aatatagctg gtaaaccgta taagccgcat
      181 gtcgagatgg catgcggctt aatattgcca acttcaaaag gcttttaaag cctccctttt
      241 atccgcgatc gcggatatcg cagcgtttat cccgtacagc ggataagatg tgtttccaga
      301 ttgactttat cctcactaaa ggataaaact gataatatcc ccttaagcgg ataaatttgc
      361 tgtggacgta tgacatgatg agctttcaga agatctatag cccaacgcaa ttggcgaatg
      421 caatgaaact ggttcgccag caaaatggct ggacgcagag cgagctggcg aaaaaaattg
      481 gcattaagca ggcgacgatt tccaatttcg aaaacaaccc tgacaatacc acgctgacga
      541 ccttttttaa gattttacag tcgcttgaac tctcaatgac gctatgcgac gcgaaaaatg
      601 cctcgccaga agcaacagaa cagcaagatc tggagtggta atgcctaaac ttgtcacttg
      661 gatgaacaac cagcgggtag gcgagttaac gaagttagcc aacggcgcgc acacctttaa
      721 gtatgcaccg gagtggttag ccagccgtta tgccagaccg ttgtcacttt cgctgccatt
      781 gcagaggggg aatatcacct ctgatgccgt atttaacttc ttcgataacc tgttacccga
      841 tagcccgatt gtacgtgacc ggatcgttaa acgttatcat gccaaatcca gacaaccgtt
      901 tgatttgttg tcagaaatag ggcgagacag cgttggtgcc gtgacgttaa tacccgaaga
      961 cgaaaccgta acgcgtccga taatggcatg ggaaaagctt actgaagcca gacttgaaga
     1021 agtattaacg gcttataaag cagatatccc gctaggcatg attagagaag aaaatgactt
     1081 tcgcatctcg gttgctggcg cacaggagaa gacagcactg ctcagaatag gcaatgactg
     1141 gtgcattccg aaaggaataa cgccgacgac gcacatcatt aaattaccga ttggcgaaat
     1201 caggcagccc aatgcgacgc tcgatctcag ccaaagcgtt gataatgagt attactgtct
     1261 gctgctggcg aaagaacttg ggttgaatgt tccggacgca gaaatcatta aagcgggaag
     1321 ggtgcgcgcg ttagcggtcg aacgttttga caggcgttgg aatactgagc gaacggtttt
     1381 acttcgcttg ccacaggagg atatgtgtca gacattcggt ttaccttcat cggtgaaata
     1441 tgaatcagat ggaggcccag gcatcgcgcg gatcatggcg tttttgatgg ggtccagcga
     1501 ggcgctgaaa gatcgctatg attttatgaa gtttcaagtg ttccagtggt tgattggcgc
     1561 aacggatggc catgcaaaaa acttctccgt atttattcag gctggcggca gttatcgact
     1621 cacgccattt tacgacatca tttcagcatt tccggtcctt ggcggtacgg gaataacatc
     1681 agcgatctca aactggcaat ggggcttaac gcatccaaag gcaaaaaaac ggcaatcgat
     1741 aaaatttatc cgcgacattt tttggcgaca gcaaaggtgc tgagattccc ggaagtgcag
     1801 atgcatgaaa tcctaagtga ctttgccaga atgattccgg cagcactgga taacgtgaag
     1861 acttcattac cgacagattt tccggagaac gtggtgacgg cagttgaaac caatgtgttg
     1921 aggttgcatg gacggttaag ccgagaatac ggtagtaagt gagatatggg gtttggtcgt
     1981 tgttaatgag caatcatgac cggccaacga ctggattctc ccttggttta tgtttgtgcg
     2041 cataatgtat attatgttaa atcatgtctg agtgatgcga agtgcttttc tggtagtcgt
     2101 tattcgttca gattaggtgt ccttgtgatt taaagatgtt atcgtggcac gaatatactg
     2161 ccattccagg ctcgttatat tgctctcggg cagtccgtag ctggtactgc aaacgctgat
     2221 gcgaccttca aagttcaata tctataattc aatcttaagg tggctatacg tcatctgaaa
     2281 ttgaaacagg gtgtgtgtgg catggctacg cacacccgag caattcacca atgtgaatct
     2341 cattattaat gaggagtggt ttgtatttgg agcgctatga aaaatcacat aatatctaaa
     2401 ct
//
LOCUS       DAZPAC010000391         2340 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZPAC010000391 DAZPAC010000000
VERSION     DAZPAC010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.399"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..138)
                     /locus_tag="ACHUPM_004515"
     CDS             complement(<1..138)
                     /locus_tag="ACHUPM_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0872082.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YE"
     gene            complement(385..858)
                     /locus_tag="ACHUPM_004516"
     CDS             complement(385..858)
                     /locus_tag="ACHUPM_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872083.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNEDAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMMVRKFGK
                     QLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHTKNELTGGKRRYRIKNGCK"
     gene            complement(1013..1603)
                     /locus_tag="ACHUPM_004517"
     CDS             complement(1013..1603)
                     /locus_tag="ACHUPM_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR0872084.1"
                     /translation="MKLIIFILIVLIIAALLIRIILKSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            complement(1641..1850)
                     /gene="hha"
                     /locus_tag="ACHUPM_004518"
     CDS             complement(1641..1850)
                     /gene="hha"
                     /locus_tag="ACHUPM_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR0872085.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(1896..2339)
                     /locus_tag="ACHUPM_004519"
     CDS             complement(1896..2339)
                     /locus_tag="ACHUPM_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR0872086.1"
                     /translation="MVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVRIRLVNI
                     DAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVNQFMVRA
                     GAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
BASE COUNT          622 a          607 c          436 g          675 t
ORIGIN      
        1 ttcgtaggcc agagttacct gcaccactgt atttcccctg tgaatattca gctcacataa
       61 ccgttccctg aatatcagtg aaaaacacaa caccgtggcg cacacggcga gcaacccgat
      121 aagggcatac ttcgtcataa actttgacct ctctgcaagg aagaggctac aatcagtagt
      181 gcaatgtact tcatgcagac ctcacgagtt aatggattaa caagtggggt ctttgcattt
      241 ctggtacctg cgtcttgcct gaaccagaaa ggctcagaca cccgccgcga taataccaca
      301 ttccctgttg ctgactatta cctgcattgc ccgcttttta ctacagaagc acgaaggcgt
      361 cactccagcc aataagtatg ctttttattt acatccattt ttaatccggt accgacgttt
      421 tcctccagtt aactcgtttt tcgtatgact catggcaata aaattttctt taccatatga
      481 caacatccca ataatatcaa agttgtcttt taatttgatg gcatcctttt cacttacagc
      541 gacaataagt tgtttaccaa attttctgac catcattgca aactgtgata ctgctgatcc
      601 accacctaaa tcaggccacc cagcacaatt aaaaatgatt gtctgctctt cactattcat
      661 tgcatccctg ataaaggaga cagcatcctc atttctctct acatctgaat cactgttaat
      721 ctgagtgaaa tcataatatt tagaacctgg tatagaatac cccttaaaaa acttatcaat
      781 gtgggaggca gagttcccaa caataagaat attgtgttca cgcttaatca tcaattctga
      841 tacatcatca tatttcatat attcaccatt attatataaa gtttgggtac aggtggttcc
      901 ggaatttact ctgtgaccac catttcccgc tttttattac agaaacgccg ggagcgggga
      961 aaacttgctt tttcccgttt ccggggttgg acaactgagc aacgcgaagg cgtcagctac
     1021 gatgttccgg ggactgctga tcagcccctg ttgtattcag ccattctccg gtcatctgca
     1081 gcagttttct ggcatcgtgg cttctgagca acggaatacc agcctgcctc agaacctctt
     1141 ccagaagaat atcccggcgc cggcgttccg gtcgtaaatg gctggcatca tccagctcta
     1201 ctgctgcaac aatactgaac gaacgccgtt caacgataac cacatcaaca tgccactgag
     1261 acaccatcct gaataactgc caccactggc gtgatcgtgg ccggatattc ccgttcagct
     1321 ggacgatatc cgcgacccgt acctgcggac acagatacca ccgcttcata tccacaattc
     1381 tgaacagtcc tttcataaaa ctgacttccc tttctgtcag cagcgtcttc tgacgttgcc
     1441 agtattcacc gccggacagt attcgctctg catcaccggt ccgtatacct gccgcatgca
     1501 actgcatcag caaaggagaa tgctgattta cacttttgag gataatccgg ataagtaacg
     1561 ctgcaataat cagaacaatg agaataaaaa taatcagctt cattgtttat ctgctcctgg
     1621 taattaatta ttctgaatta ttaccttaca tatttccata tctcttttgg tatcctgtca
     1681 tataactttc cggtgattaa ttccgctaac cggtggtctg ctgctgaatt aaatgattca
     1741 cgttcactgt ttaataaact gtctctgttt ttatgaataa ttttttccag cgtattgact
     1801 gatgaacatc gccgtaactg atacagccat tcctgttttg ttttcgccat ttttattccc
     1861 gctgagaaca gatgaatgat aagattgcca tcgctttatt tacggtgtct ccatatccag
     1921 gggggaaccg gatcagcatc tgaccagagc ccacgttttt gttgtctggc ttcgttctga
     1981 aggacaggca gcacgggatc tgtgttgtac tgctcatata cccaggcagc cccggcccgg
     2041 accataaact gattaacatt cattccgtct ggtgcataaa cctgcccgag aatacgacca
     2101 taacgatctc gctgaaaata tgtgacggta accgtttttc ctgcaaccag tgatttcatc
     2161 atgtccgttg accaccggcc ataatcctgt ttcttttccg gtgcatcaat atttaccagc
     2221 ctgatccgca ctgccttccg tgagtccatg acctcgatgg tatcaccgtc cagtacacga
     2281 acaacccgtc catgaatatc tgcacacaat accggccatg aaaagataaa taataccagt
//
LOCUS       DAZPAC010000392         2331 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZPAC010000392 DAZPAC010000000
VERSION     DAZPAC010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.400"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            172..798
                     /locus_tag="ACHUPM_004520"
     CDS             172..798
                     /locus_tag="ACHUPM_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR0872087.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            819..>2331
                     /locus_tag="ACHUPM_004521"
     CDS             819..>2331
                     /locus_tag="ACHUPM_004521"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFR0872088.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFI"
BASE COUNT          622 a          498 c          614 g          597 t
ORIGIN      
        1 tttctcctaa ccataaagga ttggtttatc ggcgttaaat cttttctcat acactatcgg
       61 gttagggaaa atatctggtg ttggggacag agtcagcagc gtattagttg aatgcttaat
      121 atcaggctgc tgaattgtca gcactgtgtt tgcgaagagg atgcgaaata aatgaacccg
      181 gttgatcgtc cactattaga tattggctta acgcgattag aattcttgcg tatatcagga
      241 aaaggccttg ctggtctaac aattgcccct gcgttactgt cacttttggg ttgcaaacag
      301 gaagatattg atagcggcac agtagggttg ataaatacac ccaaaggggt gctggtgacc
      361 cagcgagcgc gatgcacggg ttgccaccgc tgtgaaatct cttgtaccaa cttcaacgat
      421 ggctcagtag ggacattctt ctcccgtatc aaaatccatc gcaattattt ctttggcgac
      481 aacggggttg gctctggcgg cggcctgtat ggcgatctca actataccgc ggacacctgc
      541 cgtcaatgca aagagccgca atgcatgaac gtctgcccga ttggtgcgat tacctggcag
      601 cagaaagaag gctgtattac cgtcgatcat aaacgctgta ttggctgtag cgcctgtact
      661 acggcatgcc cgtggatgat ggccaccgta aataccgaaa gtaaaaaatc ctcgaaatgt
      721 gtgttatgcg gtgaatgcgc aaacgcctgc ccaacagggg cgttaaaaat tatcgagtgg
      781 aaagatatta ctgtgtgaat cttgcaaagg aaaaaatcat ggctaacggt tggacaggta
      841 atatattaag agtcaatctc acgacaggaa atattaccct cgaagattcc agtaagttta
      901 aaagttttgt cggtggcatg ggcttcggct acaaaattat gtatgacgaa gtaccgccag
      961 gcacgaaacc tttcgatgaa gcgaataaat tagtctttgc taccggccca ttaactggat
     1021 ctggtgcccc ctgtagttct cgcgtaaata tcacctcact ttctactttt accaaaggaa
     1081 atttagtcgt cgatgcccat atgggtggct tttttgcagc gcaaatgaaa ttcgctggat
     1141 acgacgtcat tattatcgaa gggaaggcga aatcaccggt atggctgaag attaaagatg
     1201 acaaagttag cctggaaaaa gccgatttct tatggggaaa agggacgcgc gcaacgacgg
     1261 aagaaatttg tcgattgacc agtccggaaa cctgtgtggc ggctattggt caggctgggg
     1321 aaaaccttgt tcctctctct ggcatgttga atagccgtaa ccacagcggc ggtgcgggaa
     1381 ctggcgcaat aatgggttcg aaaaacctga aagcgattgc ggttgaaggg acgaaagggg
     1441 tcaacattgc cgatcgtcag gagatgaagc gtctcaacga ttacatgatg actgaactta
     1501 ttggtgcgaa taacaaccat gtcgtgccaa gtacgccgca atcgtgggca gagtattcag
     1561 atcccaagtc acgttggaca gcacgtaaag ggctgttttg gggcgcggct gaaggtggtc
     1621 cgattgaaac gggggaaatt ccgccaggca atcagaacac ggtcggcttt cgtacctata
     1681 aatccgtttt tgacttaggt ccggcggcag agaaatacac agtaaaaatg agtggctgcc
     1741 actcttgccc gatccgttgt atgacccaaa tgaatattcc tcgggtgaaa gagtttggcg
     1801 tgcccagcac aggtggtaac acttgtgtag ccaactttgt ccataccacc atcttcccga
     1861 acggtccgaa agattttgaa gataaagacg atggtcgtgt gattggtaac ctggtgggtc
     1921 tgaatctgtt cgatgactac ggcctatggt gtaactacgg gcagttgcat cgcgacttta
     1981 cctattgtta cagcaaaggt gtgttcaagc gtgttctgcc tgctgaagag tatgcggaaa
     2041 ttcgctggga tcaactggaa gcgggtgacg ttaacttcat taaaattaaa gatttttact
     2101 accgtctggc gcatcgtgtg ggtgagctga gtcacctggc tgatggttca tatgccatcg
     2161 cagaacgctg gaatttgggt gaagagtact ggggctacgc gaaaaataaa ctctggtcgc
     2221 cgtttggcta tccggttcac catgccaatg aagcgtcagc gcaggtcggt tccattgtta
     2281 actgtatgtt caaccgtgac tgcatgacgc atacccatat caactttatt g
//
LOCUS       DAZPAC010000393         2330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZPAC010000393 DAZPAC010000000
VERSION     DAZPAC010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.401"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            545..1543
                     /locus_tag="ACHUPM_004522"
     CDS             545..1543
                     /locus_tag="ACHUPM_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR0872089.1"
                     /translation="MKNPELKNLTDYSPADKPWDEHRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVTEHPGARWLFLTLTVRNCSIDALGETLTAMNAGFKRLKERKEFRHVLGWVRTTEVT
                     RGKDGSAHPHFHTLLMVPPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRK
                     PKDDESLPCVVAEQLRSAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGAL
                     KNVLQLERETNEDLIMADGMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRQLSGED"
     gene            complement(1837..2271)
                     /locus_tag="ACHUPM_004523"
     CDS             complement(1837..2271)
                     /locus_tag="ACHUPM_004523"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872090.1"
                     /translation="MAVRARLYDYTSCPDFCGFVSPEHWRNVGSFCAAPPSTEARSSG
                     NRTANHSAVFVPQLFRAIDKLAVGESHEVRTYTISTSFRLCAVCDAVKTADNRQKNPP
                     VSGGRQSQFPDDSCFEVLLYPLNLPQEHSKNNSDDGTGCVIV"
BASE COUNT          531 a          547 c          676 g          576 t
ORIGIN      
        1 aaaaaaaaat tctgcgtccc gctaactgtt cccgaactcg tcaaacagaa aatcgcgggg
       61 gcccctccgg ggttccgatt ttgtgattga cgacggcacg gaaaaaaaca ctggggatgg
      121 tggttggtgt cacgcagtga ggccaaccac ctgcaagacg gtttttgttt ttcacaaaat
      181 cggtggaaaa acctgtgagt gttttgcggg gaaaacgctt ttgtggttgt tgtttttgtt
      241 gtccgaaaat cagctcagat tttgagcaaa tctcgcgcgc gttccttgta tttatactta
      301 agggataaat gcgatttttt gaatcgcctc tatcccttgc catatctggc ctgagcgcgg
      361 atttgcgtgt aaataggttt gagagtattt tgtcgggagt ggttataaat tagcccctgc
      421 gagtcttcca ccactccagg ggcgcgacca cctagaacat gaactgattt gacgacaatt
      481 ggtacaggtg atacaggaat gataacacgc aatttcgtct gtcgctcgca aggggacggc
      541 agacatgaaa aatcctgaac ttaagaatct gacggattac tctccagcag ataaaccgtg
      601 ggatgaacac cgaagctttt ccgacgatgt gggcggtatt tatctgcgtg ctgccggatt
      661 tgagcactac ggcgaacgtg tcggggcttg tagtggtatt ttgcggtttg ggtggtcaac
      721 aaacacagag accggagaaa cgcgcctgcg gttgcgtgat gcacggttct gtcgcgtccg
      781 gcactgtccg atctgtcagt ggaggcgttc catgatgtgg caagcccgtt tctatcagtc
      841 cctgccaaag attgtgacgg aacatcccgg cgcgcgctgg ttgtttctca cactgacagt
      901 gcgcaactgt tccattgacg cgcttggtga gacgctgacc gcgatgaatg cggggtttaa
      961 gcggttgaaa gagcgtaagg aatttcggca tgtgttgggc tgggttcgta ccacggaagt
     1021 aacgcgcggc aaggatggta gcgcacatcc ccattttcac actctgctga tggttccccc
     1081 ttcgatgttg tccggccgtg agtatgtgaa acatgcccgt tgggtggaac tgtggcgtga
     1141 ttgtctgcgg gtggcgtatg acccgaatgt ggatatcaga gtggtgaagt cacggaaacc
     1201 aaaagacgat gaatctttac cttgtgttgt tgcggaacag ctgcgcagcg cggtggttga
     1261 aactctgaaa tattccacaa agcctgctga tatgctggct gatcctgaat ggttcctcga
     1321 attaacacgg caggtacaca agcgtcgttt tgttgctacg gggggagcgc ttaagaacgt
     1381 tctgcaactg gagcgggaaa cgaacgagga tttaatcatg gcggatggca tggcagaggg
     1441 tacagatgac ggttcccgtc tggcgttttc gtgggtgccg gttgtcaaaa aataccagcg
     1501 agatcgggcg aaagataaac gtcagctaag tggcgaggat tgagcgcgtt tcaggcgcga
     1561 actattcatg cactgggaga atattcagaa acgcgctcag aagcgttcta acgtgatttt
     1621 tgcagacagg caaggttttg ttcgtttttt cgcgtttgat gcgttcaggg gtgttttaag
     1681 cgcgtttctg tcgcatcccg gcgctaatac catccccatg gttaacggga gctccagaaa
     1741 cgcgcagatg tctttttaac gtgtcccggg caggttgtcg agagtgtcgg ggcgcagccc
     1801 tgaccaggtg gtaatcggat ggctgtgcgt gcgcggttat acgattacac atcctgtccc
     1861 gtcatcggag ttgtttttgc tgtgttcctg cggcaaattg agcgggtata gcaacacctc
     1921 aaaacaacta tcatcaggaa attgactttg acggccccct gacacagggg gatttttttg
     1981 cctgttatct gcggttttca ccgcgtcgca gaccgcgcac aatcgaaacg aagttgagat
     2041 tgtataagtg cgcacttcgt gagattcccc gacagccagt ttgtcgatcg cgcggaacag
     2101 ctgcggcacg aaaacggcag aatgatttgc tgtccggttg ccagaactgc gcgcttccgt
     2161 ggagggcggc gcggcgcaaa aactgccgac attgcgccag tgttccggtg acacaaaccc
     2221 gcagaaatcg ggacaggatg tgtaatcgta taaccgcgca cgcacagcca tccgattacc
     2281 acctggtcag ggctgcgacc cgacaccccg ccaacgcttc acggcacgcg
//
LOCUS       DAZPAC010000394         2329 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZPAC010000394 DAZPAC010000000
VERSION     DAZPAC010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.402"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            20..238
                     /locus_tag="ACHUPM_004524"
     CDS             20..238
                     /locus_tag="ACHUPM_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR0872091.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            240..605
                     /locus_tag="ACHUPM_004525"
     CDS             240..605
                     /locus_tag="ACHUPM_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0872092.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            602..1267
                     /locus_tag="ACHUPM_004526"
     CDS             602..1267
                     /locus_tag="ACHUPM_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872093.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1267..1632
                     /locus_tag="ACHUPM_004527"
     CDS             1267..1632
                     /locus_tag="ACHUPM_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0872094.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2087..2242
                     /locus_tag="ACHUPM_004528"
     CDS             2087..2242
                     /locus_tag="ACHUPM_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0872095.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          627 a          496 c          628 g          578 t
ORIGIN      
        1 cccgaaaggg cggtaatgaa tggttacatt atttagaaaa aaatatccgc gaaagagtag
       61 aacaacagaa ttcctgtttc tcattctgtt tatcgtgttg atgacaccga tatccccgct
      121 aatttttgtc tggataatcg gaaaaataat tgagccagtt attgaattgt ataacgacgt
      181 ggtatgggcg tcattcaaca cactgcacaa taaaattaat ccgtataagg aaagctgata
      241 tggcactgac gaaaaaacaa cgtgcagaac tgcgcatgaa gttcggtggt cgctgcgctt
      301 attgcggctg cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg gtcattcgtt
      361 ttgatggaaa tatgcttcac caggaacgtg acgatatatc caacatggta ccagcatgcc
      421 acccatgcaa tctgcacaag cattgcagta gcctggaaga ttatcgacga attatcagtg
      481 atggtcgtcg tgaattcctt gcgtccggga aaggcaaagc gctggttcgt atgggattgg
      541 ttgaaatgaa atctgacccg gttgtgttct ggtttgaaaa atatcaagaa ggggctacgg
      601 catgaccact attaccaaag agcgactgca atggctggct aacatttctg gccgcgatga
      661 tattgacgat atagacggcg gtgaaattcg tgaactggcg cttatcgctc tggcatcact
      721 ggaagcagag cctgtagcgg aatgcattgt tgaagatggg ggcatgtgta ttgacgggtt
      781 cggtgagtat gtgggtcact cgctgcctga tggaatgcat gagctttatg ctgccccgcg
      841 aatgcgacaa cctgatggat ggaaagccgt agctgtagcg tggaaggtga cgtttactca
      901 agttgaccag gaatctaata cgttcactgc tatatatttt gacaaagcgg aagttgagtg
      961 ttgggtacga ctgcataaag catgtgattt tcgggcagat ataacaccgc tttacgcagc
     1021 gccggcagtg ccggttgcag taaacgacga catggcttac gcattccatc acgcactgtc
     1081 agattcatcg cttggctctg atgaaatcga agaaattaaa accggtttgc gtgctgcctt
     1141 tgccaacgtc actatccaac cagagccgat agtgccggat gaaatcgggc caaacgatag
     1201 taatacgttt gattatgttg atggctggaa cgcctgccgc gctgccatgc ttaagggaga
     1261 taaataatga ttaatcgaac caaactggag cacattctcg aatatgccag gcagcagaaa
     1321 tgtattgggc aactttgtaa aattccacca ggagatatgg ttgaaatcgt ggaaatggcc
     1381 atgcgtaagg ctggcaactc tccggtagct ccggctggct ggataagctg tagtgatgca
     1441 gttcctgctg aatattgcga tgtgattctt ctcgatgatc tcgggaatgt attccccggt
     1501 tcctgggata aggttttttg ccccactcgt ggcgggaata agatggcttt tgtggacaaa
     1561 gacggcgttg aagtagagag ctcaactcac tggatgccgc taccggaacc accgcaggag
     1621 gtgaatcaat gacctggcct gaggcattca ccacggtagg aattgcgatg gcggtggcgc
     1681 tggtggtgta ttcgatttgc cgttggggtt aacaaacaaa aaccccggat tgatggtccg
     1741 gggtttttga aggaaacaaa acagaaacag caattgccgt tacctgttgt taccatggca
     1801 agtaaacgta tctcaggcga gcgcattgcg ccgttctgac gcagataaac tagcctggat
     1861 agatggtgct ggcaataaaa aatagcgttt tcttatcggt gtcggtaaga ttgctgcggg
     1921 tgcttgaggc tgtctgcctc gggtatgtca ctgtaaggca gacagagaaa agccccagtt
     1981 aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta tgtctcacaa
     2041 atgtaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     2101 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc attgtgacgg cactggtaac
     2161 gaggaaagac ctctgcgagg tacgaatccg aatcggccag acggaggtcg ctgttttcgt
     2221 agactacgaa tctagagagt aagagtgacc aggcgggaga gtaatctccc gccacctttg
     2281 atgtgtcagg catcctcaac gcacccgcac ttaacccgtt tcggcggtt
//
LOCUS       DAZPAC010000395         2296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZPAC010000395 DAZPAC010000000
VERSION     DAZPAC010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.403"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..431)
                     /locus_tag="ACHUPM_004529"
     CDS             complement(<1..431)
                     /locus_tag="ACHUPM_004529"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0872096.1"
                     /translation="MVKSMKTNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIR"
     gene            533..757
                     /locus_tag="ACHUPM_004530"
     CDS             533..757
                     /locus_tag="ACHUPM_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0872097.1"
                     /translation="MQQEPNGWKTVTTQFYIQEFSEYIAPLQDAVDLEIATEEERSLL
                     EAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
     gene            869..1132
                     /locus_tag="ACHUPM_004531"
                     /pseudo
     CDS             869..1132
                     /locus_tag="ACHUPM_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701870.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(1169..1726)
                     /locus_tag="ACHUPM_004532"
     CDS             complement(1169..1726)
                     /locus_tag="ACHUPM_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0872098.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1723..1998)
                     /locus_tag="ACHUPM_004533"
     CDS             complement(1723..1998)
                     /locus_tag="ACHUPM_004533"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR0872099.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            2183..2296
                     /locus_tag="ACHUPM_004534"
                     /pseudo
     CDS             2183..2296
                     /locus_tag="ACHUPM_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
BASE COUNT          651 a          471 c          543 g          631 t
ORIGIN      
        1 ttgcgtatta cacagctttt tgtagatata tttccaggca ggcagtgaag aaggtaaaac
       61 atggaaatgg aatcaaattg accatgtaac gccgcgggat aaggttcaaa aacatcatgg
      121 ctaattttat gtttaatttt tgattcccca gcccttgtag atgccgcgtt caggctagct
      181 tcgttcaaat ccattaaaga tatcagacta ctctcaggta cgtgagtaag gtaaaaccca
      241 gttccaacac caatatccag atggttgtta cctaaatgtt ccagaaagtg tggaagaagg
      301 tgttcctttg taggacatcc ccatgcaagc cgatttgata ctcccaaaac ccaccagtca
      361 taaagcttta gggtaagtgg tgtgtaaatt ttagccccat catctgtgtt tgttttcatt
      421 gatttcacca tgttatagtt ttatttgtga attaaatcaa ttatggcgat gaattacaag
      481 gggttaaatg ctgccgcagc atagcgatat tgaaatagcc tagtatgctt cgatacagca
      541 ggagccgaat ggctggaaga ccgtcaccac acagttctac atccaggaat ttagtgagta
      601 tattgcgcca ctgcaggatg ctgtagatct ggaaatcgca acggaggaag aaagatcgtt
      661 gctggaggca tggaataaat atcgggtatt gttgaatcgt gttgatacat caactgcacc
      721 tgatattgag tggcctgcaa atcctgtcag ggagtaatca ttgggattat gccgcagaca
      781 cgtcgtatgc aggaacgtgc tgcggttagt ttgtgagctt tcgatagtgg ttgttatttt
      841 tgcccttatt tgttccggag gccatggttc aatggtccgt ctgccccctg tggtgatgtc
      901 agcaaaatca gccactgcgc gaaccacaat agcccgggaa gatgctgaag atcaccaggt
      961 aaagctgtca gcgcagaaac tggaagaact gctcgcatca atggttaagg atgaggttga
     1021 tcgcaatgat gggatttatt gacgtcagcg agagcagaag gaagaactga ataacctgaa
     1081 tgatttacgc tcaatcagag cgatggcgat tagcggcaat cacgctcgat aaaattaacc
     1141 tcggtgatca tccggggttt tgcattcatt aaaaccgcat caacctttcc accaattgtt
     1201 ctttacgggc aacgatccac ccatgttgct ccagataaaa tttaaaccgt tccagagtgc
     1261 ataccatcgc atcggcgggg actttttctg tgaactcgac ctgaccgtgt ttatcgaagt
     1321 ggatcagtaa tgcgcatcca ttatttgggg cgggggagtt ttgtgctgca ggaggacgtt
     1381 tgtggagttc ttcttccatt gcgttaaatt tttcaatgta agcttctttt agtctggctg
     1441 cttttttgcc agtaaatccc attacgagga acatgaagcc gtcttttgtg atcaggtagc
     1501 atcgtgattc acgttctgcg ccgttgccaa tttcgacagt ctgaacatcg gccgaaaagt
     1561 tggctgatgt aaattcggga gagcagtcga gtgtgtcaat cttagctaaa acgtgtttat
     1621 gttgtttatc gaagtagtcg gcaacagctt gagaggtggt aaccaggcga ccatctttga
     1681 aaaagacttt tggtgactcg ttagaaaaag ttatagctgt attcatatgt gatgactcca
     1741 attaattgga tgatcaccac ctctgacgcc aatcagggag gtggtgagac gtacagggtt
     1801 ggcgtaaccg gtcatcacaa aacccggcgc ggatttctcc gcccctgcac gccccaccat
     1861 aatgcgaatg tggcaatgct taacgcaaaa aaaaccgcaa acgcggctat gcgttgtgat
     1921 gttatccgag acgccaatcc cgacaaccga ttttgcggtt gtgtgaggaa tatagccccg
     1981 gagaacaaaa actgtcaacg ggcaaaaaaa tttccgttat caggaatgta taaaattaaa
     2041 gcgatcgagc aaaaaatcct ttctgcaaaa ctaaaaaaga tttcaataaa atcaggcagt
     2101 taaaaacacg gaaaacggta taaaaaagcg cctcaaaatc ctgttttttc tttcagatac
     2161 aatgtcttat tgcatttttt aaaacatgtc agtgtggtac atggatatcg ataccatcgc
     2221 caattataaa ctgggcggtg cacagcaaca tgatagcgta cgcctcgatc cgtgggtgtt
     2281 tatgttctca gcagga
//
LOCUS       DAZPAC010000396         2293 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZPAC010000396 DAZPAC010000000
VERSION     DAZPAC010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.404"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1230
                     /locus_tag="ACHUPM_004535"
     CDS             <1..1230
                     /locus_tag="ACHUPM_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFR0872100.1"
                     /translation="DLWPVKEPDMLTVIMQRLTALEQSGEMGRKMDAFKERVIRNSLR
                     PPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEGRVAGFESAYVALSRMKQHVQVYTDN
                     RQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLA
                     GGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKG
                     SGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVSIAGEGRPWNPGAITDGR
                     VWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPD
                     GKTELAVREIAGQERDRAAITEREAALPESVLRESQREREAVREVARENLLQERLQQM
                     ERDMVRDLQKEKTPGGD"
     gene            1250..1997
                     /gene="traX"
                     /locus_tag="ACHUPM_004536"
                     /pseudo
     CDS             1250..1997
                     /gene="traX"
                     /locus_tag="ACHUPM_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            2052..>2293
                     /gene="finO"
                     /locus_tag="ACHUPM_004537"
     CDS             2052..>2293
                     /gene="finO"
                     /locus_tag="ACHUPM_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR0872101.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTL"
BASE COUNT          500 a          585 c          778 g          430 t
ORIGIN      
        1 gatctgtggc cggtaaagga gccggacatg ctgacggtga tcatgcagcg cctgacggca
       61 ctggagcagt ccggtgagat gggccggaaa atggatgcct ttaaggagcg ggtgatccgc
      121 aacagtctgc ggccccctgc cgtgcccggt atcggacgca cggagaaata cagcagccgg
      181 ttgtttgacc cgtccgtcag actggctgcg gatatccggg ataacgaagg gcgggtggcc
      241 ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca ggtgtacacc
      301 gataaccgtc agggctggac ggatgccatc aacaatgccg tacagaaagg aactgcccac
      361 gatgtgtttg agccgaaacc ggaccgggag gtcatgaatg cagagcggct gttcagtacg
      421 gcgcgggagc tgcgggacgt ggcggcaggc cgtgccgttc tccgtcaggc ggggctggcc
      481 gggggagaca gtcctgcacg gtttattgct ccgggacgta aatatccgca accgtatgtg
      541 gcactgccgg cgtttgaccg taacggcaag tccgccggta tctggctgaa cccgctgacc
      601 acggatgacg gaaacgggct gcggggattc agcggtgaag gtcgggtaaa aggcagcggg
      661 gatgcgcagt tcgtggccct gcagggcagc cgtaacggag aaagcctgct ggctgataat
      721 atgcaggatg gcgtccggat tgcccgtgat aatcctgaca gtggtgtggt ggtaagcatt
      781 gccggtgaag gccgtccgtg gaatcccggt gccatcaccg atggccgcgt gtggggggat
      841 atcccggaca acagcgtcca gccgggagcc ggaaatggcg aaccggtcac ggcagaggta
      901 ctggcacagc ggcaggctga agaggccatc cgccgtgaaa cggaacgtcg cgcagatgaa
      961 attgtccgta aaatggcaga gaacaaacct gacctgccgg acggcaaaac agagctggct
     1021 gtcagggaaa ttgccgggca ggagcgtgac cgggctgcca taactgaacg ggaagccgcg
     1081 ctgccggaga gtgtgctgcg tgaatcacaa cgggagcggg aagcggtccg ggaggttgcc
     1141 cgggaaaatc tgctgcagga gcgtctgcag cagatggagc gggatatggt tcgtgacctg
     1201 cagaaagaga aaaccccggg cggagactga tacaggaaga caaacactga tgacaacgga
     1261 taacacgaac acgacacgta acgattcact ggctgcacgg accgatacct ggttgcagtc
     1321 atttctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac tggtgctgat
     1381 ggtactggac cacatcaatc tgatattcca gctgaagcag gaatggatgt ttctggccgg
     1441 gcgaggagcc tttccgcttt ttgccctggt gtgggggctg aatctgtccc gtcatgcgca
     1501 tatccggcaa ccggccatta accggctgtg gggatgggga attattgccc agttcgcata
     1561 ttatctggca ggctttccct ggtatgaggg gaatatcctg tttgcctttg cagtggcggc
     1621 acaggtgctg acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg ccattcttct
     1681 gatggccctg ttgggggcct ttgtccggca ccagttacgg cattgccggg ctgctgatgc
     1741 tggcagtcag ccaccgactg taccgggcgg aagacagagc ggaacgtctg gcgctggtcg
     1801 cctgcctgct ggctgttatt ccggcgctta accttgccac cagtgatgca gcggcggtgg
     1861 ccggtcttgt gatgacggtg ctgaccgttg gtctggtgtc gtgtgcagga aaatcattac
     1921 cccgtttctg gcccggggat tttttcccgg tattctacgc ctgtcatctg gctgtgctgg
     1981 gcgttctggc gctgtgacgg gtgtggtatc tttgaccgta acaggatgat tcgtcagagg
     2041 cagaacacag catgacagag cagaagcgac cggtactgac actgaagcgg aaaacggaag
     2101 gggagacgcc tgtccgcagc cggaaaacca tcatcaatgt caccacgcca ccaaagtgga
     2161 aggtgaaaaa gcagaagctg gccgagaaag cagcccggga agcagagctg gcggcaaaaa
     2221 aagcgcaggc cagacaggca ctgtccattt atctgaacct gcccacgctg gatgaggccg
     2281 tgaataccct gaa
//
LOCUS       DAZPAC010000397         2287 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZPAC010000397 DAZPAC010000000
VERSION     DAZPAC010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.405"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(87..296)
                     /gene="fumD"
                     /locus_tag="ACHUPM_004538"
     CDS             complement(87..296)
                     /gene="fumD"
                     /locus_tag="ACHUPM_004538"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR0872102.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            651..845
                     /locus_tag="ACHUPM_004539"
     CDS             651..845
                     /locus_tag="ACHUPM_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872103.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            853..2265
                     /gene="pykF"
                     /locus_tag="ACHUPM_004540"
     CDS             853..2265
                     /gene="pykF"
                     /locus_tag="ACHUPM_004540"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR0872104.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          629 a          563 c          501 g          594 t
ORIGIN      
        1 ttcagcagtt gttatttaga atgattaact ggaccgcaac tgaagcaaga atatcaggat
       61 gaagcttacc gcctcatcct gaatggtcat tttaccagtg cgttaatgac ggtttccatc
      121 gcctgctttt ccagctcact gcgctgaatg cgtttattgg ctaatgcagt acgtaatacg
      181 cctgcgataa caatatgctt tggttcctgc cccagatcgc gcatttccag cacaacttta
      241 ccgacaactc tgcacatttc tcggtacagt tcatcctctt ttgtccgatt tcccattctt
      301 tatacctatt tatcatgcca actatcagca tatatcaatc taaccaatta aacaaaaatc
      361 aaacaaaatc agacaaataa cgcgataaat tattttaact gtagcaattg agcgatgata
      421 tatttataca ccggatgaac tttcacttat cctcacactg acaacttcgg caccagacgt
      481 tgcgcaaaca gcgaagtttt tgcgtaacct tttccctgga acgttaaatc tttgataaca
      541 atttattgtc taacaagttg tatatttttt gaaacgctgt ttttgttttc cttttggatt
      601 aatttcagcg tataatgcgc gccaattgac tcttgaatgg tttcagcact ttggactgta
      661 gaactcaacg actcaaaaac aggcactcac gttgggctga gacacaagca cacattcctc
      721 tgcacgcttt ttcgatgtca cctatcctta gagcaaggca ccaccacttt cgtaataccg
      781 gattcgcttt ccggcagtgc gcccagaaag caagtttctc ccatccttct caacttaaag
      841 actaagactg tcatgaaaaa gaccaaaatt gtttgcacca tcggaccgaa aaccgaatct
      901 gaagagatgt tagctaaaat gctggacgct ggcatgaacg ttatgcgtct gaacttctct
      961 catggtgact atgcagaaca cggtcagcgc attcagaatc tgcgcaacgt gatgagcaaa
     1021 actggtaaaa ccgccgctat cctgcttgat accaaaggtc cggaaatccg caccatgaaa
     1081 ctggaaggcg gtaacgacgt ttctctgaaa gcgggtcaga cctttacttt caccactgat
     1141 aaatctgtta tcggcaacag cgaaatggtt gcggtaacgt atgaaggttt cactactgac
     1201 ctgtctgttg gcaacaccgt actggttgac gatggtctga tcggtatgga agttactgcc
     1261 attgaaggta acaaagttat ctgtaaagtg ctgaacaacg gcgacctggg cgaaaacaaa
     1321 ggtgtgaacc tgcctggcgt ttccattgcc ctgccagcac tggctgaaaa agacaaacag
     1381 gacctgatct ttggttgcga acaaggcgta gactttgttg ctgcttcctt tattcgtaag
     1441 cgttctgacg ttatcgaaat ccgtgagcac ctgaaagcgc acggcggcga aaacatccac
     1501 atcatctcca aaatcgaaaa ccaggaaggc ctcaacaact tcgacgaaat cctcgaagcc
     1561 tctgacggca tcatggttgc gcgtggcgac ctgggtgtag aaattccggt agaagaagtt
     1621 atcttcgccc agaagatgat gatcgaaaaa tgtatccgtg cacgtaaagt cgttatcact
     1681 gcgacccaga tgctggattc catgatcaaa aacccacgcc cgactcgcgc agaagccggt
     1741 gacgttgcaa acgccatcct cgacggtact gacgcagtga tgctgtctgg tgaatccgca
     1801 aaaggtaaat acccgctgga agcggtttct atcatggcga ccatctgcga acgtaccgac
     1861 cgcgtgatga acagccgtct cgagttcaac aatgacaacc gtaaactgcg cattaccgaa
     1921 gcggtatgcc gtggtgccgt tgaaactgct gaaaaactgg atgctccgct gatcgtggtt
     1981 gctacccagg gcggtaaatc tgctcgcgca gtacgtaaat acttcccgga tgccaccata
     2041 ctggcactga ccaccaacga aaaaacggct catcagttgg tactgagcaa aggcgttgtg
     2101 ccgcagctgg ttaaagagat cacttctact gatgatttct accgtctggg taaagaactg
     2161 gctctgcaga gcggtctggc acacaaaggt gacgtcgtag ttatggtttc tggtgcactg
     2221 gtaccgagcg gcactactaa caccgcatct gttcacgtcc tgtaatattg cttttgtgaa
     2281 ttaattt
//
LOCUS       DAZPAC010000398         2248 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZPAC010000398 DAZPAC010000000
VERSION     DAZPAC010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.406"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(110..1114)
                     /gene="argF"
                     /locus_tag="ACHUPM_004541"
     CDS             complement(110..1114)
                     /gene="argF"
                     /locus_tag="ACHUPM_004541"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR0872105.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1276..1692
                     /gene="rraB"
                     /locus_tag="ACHUPM_004542"
     CDS             1276..1692
                     /gene="rraB"
                     /locus_tag="ACHUPM_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR0872106.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1738..2241)
                     /locus_tag="ACHUPM_004543"
     CDS             complement(1738..2241)
                     /locus_tag="ACHUPM_004543"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0872107.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          571 a          555 c          548 g          574 t
ORIGIN      
        1 tacgtatagc aataaaaaaa ttggggtgga ataattgaac tatcactgca taaaataatg
       61 tgacgccggg atgtttttgt atttcccggc atctttatag cgatatcaat tatttactga
      121 gcgtcgcgac catcaccgct ttgatggtat gcatgcggtt ttccgcctga tcaaatacaa
      181 tgctggcggc agattcgaag acctcatcag tcacttccat tccgccatgt aggccaaact
      241 cttccgccat ttttttgcca agcgtcgttt ggtcgtcatg aaacgcgggc aggcagtgga
      301 ggaatttgac ctccgggtta ccggtcaact gcatcatctt gctgttcact tgataatcac
      361 gcagcaatgc aatgcgttca gcccatttct cttttgcttc acccatcgac acccatacat
      421 cggtatagat aaagtcagca ccttcaactc ctttagctac atcttccgtc agcgtaatat
      481 tcccgccatt ttgttgtgcc agggcgcggc attccgtaac cagcgcagct tccggccagc
      541 atgcctgtgg cgcgaccaga cgcaaatcca gaccggtaag cgctgcagct tcgagcatcg
      601 aattgcccat gttgttacgt gcgtcacctg catagactag cgtcatttcg ttgaacgttt
      661 tgccgggcaa atgctcctgc atggtgagga gatccgccag cagctgcgtg gggtgaaact
      721 cattggtcag gccgttccat accggcacgc cagcgtattc cgccagtgtt tcgacaatct
      781 cctgaccata gccgcgatac tgaataccgt catacatgcg accaagcacg cgggcagtgt
      841 ctttaatcga ctctttatga ccaatttggc tgccgcttgg gccgagataa gtaacacgag
      901 ccccctggtc atatgcggca acttcgaaag agcatcgggt acgggtcgag tctttttcga
      961 agatgagcgc gatgttttta ccagtgagtt tggcttcttc tttaccgctt ttcttatcgg
     1021 ctttcagctt cgcggctaac tgcagcaggc tgttgagttc agctggcgtg aaatcgagta
     1081 atttcaggaa atgcttatga taaaacccgg acataggtcc ctcctgtggc taacgcctta
     1141 atgaattaaa attcaattta tatggatgat tattcatttg caagtctaaa gcataaatct
     1201 ttatcacaaa ggtggaggca atgtcagtgg tgtgtgacaa taagagtatc ggcaggacat
     1261 taagaggaat gagccatggc aaacccggaa caactggaag aacagcgtga agaaacacgt
     1321 ttgattattg aagaattact ggaagatggc agcgatccgg acgcgttgta caccatcgaa
     1381 caccatcttt ccgcagacga tctggaaact ctggaaaaag cggcagttga agcgtttaaa
     1441 ctcggttacg aagtgaccga tccagaagag ctggaagttg aagatggtga tatcgtgatt
     1501 tgctgcgaca tcctcagcga gtgcgcgttg aatgccgatc tgatcgatgc ccaggttgaa
     1561 caactgatga cgctggcgga gaaatttgac gttgagtacg acggttgggg cacttacttt
     1621 gaagatccta acggcgaaga tggcgacgat gaagattttg tcgatgaaga cgatgacggg
     1681 gttcgccact aattaattga ttaaaatgcc cgatagcaca tactagcggg cattttttta
     1741 gagttcgcgt agcatccgca cttcacaatc gacatggccc gtgcagccaa gcgcatagtc
     1801 gatatgctca aagcccaaat gctcataaag cgcaatggct tcctttaaaa aagcggtcgt
     1861 ttccagatag cagcgtttga agcccatctc tcgtgcctgc tccatcgcca ttaaggccag
     1921 ttttttcgct agccctttac cgcgaatagc cggaagaaaa tacatctttt gcagttcgca
     1981 aatgtccgac tcactcccgg ttaatggcgc aatcccaccg ccgccgacca cttcaccctc
     2041 gtactcaacc acccaatatg catggccagg ctgactatac acttgataca gctcgtcaag
     2101 attcggatcg gcgacggtgt agcctttatc agcggtaaga ccgtattcgg cggatacctg
     2161 acgaatgacg cgggcgatag caggattatc cagcagcgtc aggcgacgca ttacaggtga
     2221 ttgcggcgca atgttattca tagtttaa
//
LOCUS       DAZPAC010000399         2194 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZPAC010000399 DAZPAC010000000
VERSION     DAZPAC010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.407"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..255)
                     /locus_tag="ACHUPM_004544"
     CDS             complement(<1..255)
                     /locus_tag="ACHUPM_004544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872108.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEIIKTQVFHWLIYSH"
     gene            192..1691
                     /gene="pitB"
                     /locus_tag="ACHUPM_004545"
     CDS             192..1691
                     /gene="pitB"
                     /locus_tag="ACHUPM_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR0872109.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1740..>2194)
                     /locus_tag="ACHUPM_004546"
     CDS             complement(1740..>2194)
                     /locus_tag="ACHUPM_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HFR0872110.1"
                     /translation="VPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAY
                     DGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRES
                     LARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
BASE COUNT          517 a          528 c          573 g          576 t
ORIGIN      
        1 atgtgaatat attaaccagt ggaatacctg tgttttgatt atttctaaag gttttgaatg
       61 aatgcttatt gtctgataca caagaaataa cactcttttt atcgttaaaa aatgatattt
      121 cactttgccc atgccgttaa agtcactgat aatgcgtccg ttcgtaaatt caaaatggcg
      181 taatctaata tatgctaaat ttatttgttg gccttgatat atacacaggg cttttgttat
      241 tgcttgctct ggcatttgtg ttgttctacg aagcaatcaa tggttttcat gacacggcga
      301 atgcggtggc aaccgttatt tatactcgtg ccatgcaacc acaacttgct gtggtgatgg
      361 cggcattttt taactttttt ggcgtgttat tgggcggact tagcgttgcc tatgccattg
      421 tccatatgtt gccaaccgat ttgttgctga atatggggtc aacccacggc ctggcgatgg
      481 tcttttccat gctgctggcg gcgattatct ggaacctggg aacgtggttc ttcggtttac
      541 cggcctccag ttcgcacacc ttgattggtg cgattatcgg catcggttta accaacgcgc
      601 tgttaaccgg ctcatcggtg atggatgcgt taaacctgcg tgaagtgacc aaaattttct
      661 cctcgctgat tgtttcccct atcgtcggcc tggtcattgc gggaggcctg atattcctgc
      721 tgcgacgcta ctggagcggg acgaaaaagc gtgaccgtat tcaccgcatt ccggaagatc
      781 gcaaaaagaa aaaaggcaaa cgtaaaccgc cattctggac gcgtattgcg ctgattgttt
      841 ccgctgcggg cgtggcgttt tcgcacggcg cgaacgacgg acaaaaaggg atcggcctgg
      901 tgatgctggt actggtgggg attgcccctg ctggcttcgt cgtcaatatg aacgcgtccg
      961 gctatgaaat tacccgtacc cgcgatgccg tcaccaactt cgaacactac ctgcaacagc
     1021 atcctgaact gccgcagaag ttgattgcga tggaacctcc attgcctgca gcatcgactg
     1081 atggcacgca agtaacagag tttcactgtc atccggcaaa tacctttgat gcgattgcgc
     1141 gcgttaaaac gatgctgcca ggcaatatgg aaagttacga gccgttaagc gtgagtcagc
     1201 gcagccagct gcgccgcatt atgctgtgca tctctgatac ctccgcgaag ctggcgaaac
     1261 tgccaggcgt cagtaaagaa gaccagaacc tgctgaaaaa actgcgcagc gatatgttaa
     1321 gcaccattga gtacgctccg gtgtggatca tcatggcggt agcactggcg ctcggcattg
     1381 gcaccatgat tggctggcgt cgtgtagcga tgaccatcgg tgagaagatt ggtaagcgcg
     1441 gcatgacgta tgcgcaaggc atggcggcac aaatgacggc ggcagtgtct atcggccttg
     1501 ccagttatat tgggatgcct gtctccacaa cacacgtcct ctcgtctgcg gtagcaggga
     1561 cgatggtggt ggacggcggt gggttacagc gtaaaacggt aaccagcatc ctgatggcgt
     1621 gggtgtttac tttaccggcg gcaatttttc tttctggtgg gctgtactgg atagcattgc
     1681 agttgattta accttcctga aaatgcccgg tcctggcgat cgggcatttc catttttgac
     1741 tagtgataac cacgcgcggt cataaaatcc gtaatcgctt tttctgcatc aaccaacacc
     1801 tgttccagtg gctgattggc atcgatatca accagttgtg caccaccaaa ggttaactgt
     1861 ggcgttatgg caatcttcct cgccagcgat tcccgtttat ggtcgggttt acgtgcacag
     1921 gcaacttcaa ggtcaacatt gagtttgatg accagatcag gcttatggct cgccatccag
     1981 tgaaacgctt tacgttcctg gcttgccagc catgagacaa aacgaccacc ttcaacgtta
     2041 ggtgggaaca ccgtaccatc gtaagcgcca ggaatttggt cctgaggata acggtcggtt
     2101 agaacaatta acccgcgacg acgacaggca agcatatgac gaaagcgcag taagcgacgg
     2161 gcgacaaacg ctgtaattac cagcgccgga actg
//
LOCUS       DAZPAC010000400         2171 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZPAC010000400 DAZPAC010000000
VERSION     DAZPAC010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.408"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(1..594)
                     /locus_tag="ACHUPM_004547"
                     /pseudo
     CDS             complement(1..594)
                     /locus_tag="ACHUPM_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            930..1115
                     /locus_tag="ACHUPM_004548"
                     /pseudo
     CDS             930..1115
                     /locus_tag="ACHUPM_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1215..1421)
                     /locus_tag="ACHUPM_004549"
                     /pseudo
     CDS             complement(1215..1421)
                     /locus_tag="ACHUPM_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(1474..1725)
                     /locus_tag="ACHUPM_004550"
     CDS             complement(1474..1725)
                     /locus_tag="ACHUPM_004550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872111.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGSFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
BASE COUNT          605 a          546 c          497 g          523 t
ORIGIN      
        1 tcttcataac aaatatccct ctgatatgac aacaaaaaac ggagccggat tcctgttttt
       61 gtgaactgca gctattcgag tctaaaaagt gatccactta ccaccaacgg cctaatatat
      121 acagaacgga gtaaacaggg ctaacagttc ccgacaggtc tcatcattcc gtggcccgaa
      181 agtgtaagcc aacacaccgc cagttttagt gttgtacgca taccagagcc agtgttgttg
      241 cagccttgct gccaacgaag cgccactgct catcaagttc acagataagg gcaacatcag
      301 cgtgagcgac tggcgcagat gttatccgcc ggggcgggag ctttttaatg tccgtatgac
      361 ggtattgatg ccgattttca gtgtcctggc ggtatcacgg ccccctgcgc cgttatgtgc
      421 catttcaacg atctgctcct taacgccggg ttttctggct tcataagagt aagtgagctg
      481 gaaaacacgt ttacatgagc gacaacggaa acgctcatgc cgggaacaac tacggccatg
      541 tcgataaact tcatcggaat gacagcgcgg acaatgaaca gtaacggtag ccatggaaaa
      601 ccttaaatac cagtagatta ccagaaactt ctgtcgattg aagacatcac ccgaagtttg
      661 aattcattcg aatacttttt acgaggttct tttcgccagt cctgtaattc catacttagg
      721 tgttcgtcta tgtcagatgg gcgtctaagt taccaattct tgtctaatgg ctacatacgg
      781 tggtcggttt acgcttaccg caggacaggt ggtaactgaa aaatagtttg ctgaggcgac
      841 tggaaacaga agggcaagat cgattccgac agcagtgatg gtcagctacc tcatcgataa
      901 ctttgcaaca gtgcccttct tgaagcatca tgcttcatga agaaaaactg gcacgtcatc
      961 aacgtaaaca ggcgatgtat acccgaatgg tagccttccc ggcggtgaaa atgttcgaag
     1021 agtatgactt cacgttcgcc accggagcac cgcagaagca gctccagtcg ttacgctcac
     1081 tcagcttcat agagcgcaat gaaaacatag tgttacaggg gacatctgac atcacaaatc
     1141 cccgggtggg gatttgtgta taagagacag cccatggagg aaggggatat atcgcagctc
     1201 tgacgactca ctccttattt acccgggaaa tacttctcca gcaatgcata caggatggca
     1261 gcggtttcag catccattat gccgtcataa tttttctgcc ggaaatgaag ctgaaaagcg
     1321 cggacaagcg ccttatatcc gtcatccgtc tccacaccag aaatactgta tccataggtc
     1381 ttcagccgtg acacgatatc aaccttatcc ggaatgccct gcgattcaaa aaataaatat
     1441 ttagtcagta attttatgtt tttcctgaaa aaattactct ttttcgaact ccagtgagcg
     1501 ttcacgactc atcagtgtga aaatgatggc aaaaacagca gacagtatca gtacgataaa
     1561 tgtggccccc catcccagat aatcgacaac aagtcccatt cccagtgtcg caacaattga
     1621 gccgaagata taggaaaatg agcctgacag ccctaacaga ataagtttac gcgtaaatcg
     1681 cccggaatcc agaagagcat taagtatcac ataaacaggt tccataccag tcctgaccgc
     1741 atcaggacgc cagtaaataa aatctcgtag tccaccggca tcagagacaa gagtaatcgt
     1801 cgttaccggt atttcgactg gtttgtactg gtgtgagcgg acaaaactac tgtacgtctc
     1861 tcatacacgc tcgtaagcgg ccctcacggc ccgctaacgc ggagatacgc cccgactgcg
     1921 ggtaaaccct tgtcgggacc actccgaccg cgcacagagg cattttcatc gctgacaggc
     1981 cgcatggcct cgcagagccg gagggggact ggtgatattc ggacgaccgt accggcttgc
     2041 gccgggcttc ggcggtttta cacctgtgct acatgtaacc accgtatgcc gccttatatg
     2101 cgctgactcg cattatttgc cttgttccgt ctgaaagtaa tcactatgat taaatatgat
     2161 taacaactaa t
//
LOCUS       DAZPAC010000401         2136 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZPAC010000401 DAZPAC010000000
VERSION     DAZPAC010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.410"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            11..1204
                     /locus_tag="ACHUPM_004551"
     CDS             11..1204
                     /locus_tag="ACHUPM_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0872112.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            complement(1154..2080)
                     /locus_tag="ACHUPM_004552"
     CDS             complement(1154..2080)
                     /locus_tag="ACHUPM_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFR0872113.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
BASE COUNT          471 a          519 c          523 g          623 t
ORIGIN      
        1 aaacattata atgtcaccag ggatcgaaga tacgccccaa aaaacactgt cctgctggcc
       61 acttgcgttc agtgccgggc ttctcggtat cggacagaac ggtctgctgg ttgtgctccc
      121 tgttctggtc atacagacaa atctgagtct gtctgtatgg gctgccctgc tgatgcttgg
      181 ctcaatgctg tttctgccat cgtccccgtg gtggggaaag caaatttccc gtactggcag
      241 taaacctgtg gtgttgtggg ctctgggagg atatggcata agctttaccc tgctagggct
      301 gggaagcgtg ctgatggcta ccagcgccat aacaacagcg gtgggactgg gaatattaat
      361 catcgcccgg atcgcctacg ggctgaccgt gtcggcaatg gtgccagcct gtcaggtctg
      421 ggcattgcag agagctggag aagggaatcg catggccgct ctggcaacca tcagctccgg
      481 cctgagttgc ggcaggctat tcgggccgct gtgcgcggca gcaatgctgg ccattcatcc
      541 tctggcgcca ctggggctac tgatggcagc accagtgctg gcgctgctga tgctgctgcg
      601 gttgcccggc acaccaccac agcccacacc ggagtgcaag agcgtcagtc tgaagcggga
      661 ttgtctgcct tatctgcttt gcgcaatatt actggctgcg gcggtgagca tgatgcagct
      721 tggactttcg cctgccctta ctcgccagtt cgtcacggat accaccgcca ttagccaaca
      781 ggtggcctgg ctgttgggtc tgtccgcagt agctgcgctt atcgcgcagt tcggggtact
      841 ccgtccgcag cgcctgactc cggtggccct gctcctgagt gccggagtgt tgatgagtgg
      901 tggtctggct atcatgctct ccgaacagct atggttgttt tacccgggct gtgcagtgct
      961 gtcatttgga gcagctctgg caacacccgc ttatcaactt ctactgaatg ataagctggc
     1021 tgatggcgca ggcgcgggct ggctcgctac cagtcacaca cttggctatg gactgtgcgc
     1081 tttgctggtg ccgctggtat cgaaaacagg tgtcgcaatc gcgctgatta tggcagcatt
     1141 atttgcaact atattattta ccattgtgtc tgtatttatc tggcattacc gtactatcaa
     1201 ataaagatat gatcatcacc ttactggagt aaatcagagg atatgagaga ggacctgcgt
     1261 ctgatgaaag catgtcaggc agtgttctgg cttgctgccc ccgcccatca ggtatagtga
     1321 attcaaccag ttgctgcggt gtaatctcca tgattctttc gtgcaacaac ctatgatccc
     1381 gtttaccatt aatattcagc gttgctctgt tattaaattt cattctgaca agccagtcag
     1441 cttttattcc gggagtctgc tctgcgcgtt caaacaggtc ataaaaatcg ccttcacgat
     1501 cagaaattga caatacatgg gtatcagggc attggcgagc cagttctgtt gctttattat
     1561 atccttcaac ccaacgccag gtttcttttt cactgacatg ttttttatga agtaaatcat
     1621 tgcagatttc ttgtcgggta tttttactgg tttttaattc atccctgaac cactgatagt
     1681 catcataaac accgaggcaa acacctgact cactgatgac gagttgagga tgaagaaata
     1741 agtttctttc atctttatgt tttttgtgac caactccttg tttttctttc tgaccagaat
     1801 aattaagttc tgttgtatcc tgtaaaacta aaactctttt aaactgctga gctctttcag
     1861 tgatattttc tttgtgagga gccataattt tattagcaga gactttatcg tttgaaaaac
     1921 aacgataagc tgcctttgtt tcactccagg tacggcagga aacaggaata ctttcttctg
     1981 gtgaacgact gaggctattg aggatatgtt ttactctttt atcaagtctt gagtccccta
     2041 tatccagtga ttttagctcc tgaataaccc agttttccat aacacctctg aaataatctc
     2101 tgtcttttca atataatact attatttcaa cttgtg
//
LOCUS       DAZPAC010000402         2091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZPAC010000402 DAZPAC010000000
VERSION     DAZPAC010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.411"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..216
                     /locus_tag="ACHUPM_004553"
     CDS             <1..216
                     /locus_tag="ACHUPM_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR0872114.1"
                     /translation="LRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQAGIPLLRSH
                     DARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            456..716
                     /locus_tag="ACHUPM_004554"
     CDS             456..716
                     /locus_tag="ACHUPM_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR0872115.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            940..1014
                     /gene="tap"
                     /locus_tag="ACHUPM_004555"
     CDS             940..1014
                     /gene="tap"
                     /locus_tag="ACHUPM_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR0872116.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1007..1864
                     /gene="repA"
                     /locus_tag="ACHUPM_004556"
     CDS             1007..1864
                     /gene="repA"
                     /locus_tag="ACHUPM_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR0872117.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESDAGTLSITRATRALTFLAELGLITYQTEYDPLIGCNIPTDITFTPALFAALDV
                     SEEAVASARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          533 a          534 c          549 g          475 t
ORIGIN      
        1 ctgcgttcgt tcagtattgt tgcagcagta gagctggatg atgccagcca tttacgaccg
       61 gaacgcagac gccgggatat tcttctggaa gaggttctga ggcaggctgg tattccgttg
      121 ctcagaagcc acgatgccag aaaactgctg cagatgaccg gagaatggct gaatacaaca
      181 ggggctgatc agcagtcacc ggaacatcgt agctgacgcc ttcgcgttgc ttagttgtcc
      241 aaccccggaa acgggaaaaa gcaagttttc ctcgctcccg gcgtttccat aactgaaaac
      301 catactattt cacagtttaa atcacattaa acgacagtaa tccccgttga tttgtgcgcc
      361 aacacagatc ttcgccacaa ttctcaagtc gctgatttca aaaaactgta gtatcctctg
      421 cgaaacgatc cctgtttgag tattgaggag gcgagatgtc gcagacagaa aatgcagtga
      481 cttcctcatt gagtcaaaag cggtttgtgc gcagaggtaa gcctatgact gactctgaga
      541 aacaaatggc cgcagttgca agaaaacgtc ttacacacaa agagataaaa gtttttgtca
      601 aaaatcctct gaaagatctc atggttgagt actgcgagag agaggggata acacaggctc
      661 agttcgttga gaaaatcatc aaagatgaac tgcagagact ggatatacta aagtaaagac
      721 tttactttgt ggcgtagcat gctagattac tgatcgttta aggaatttta tggctggcca
      781 cgccataagg tggcagggaa ctggttctga tgtggatgta caggagccag aaaagcaaaa
      841 accccgataa tcttctttaa ctttggcgag tcagaaagat taccggggcc cacttaaacc
      901 gtatagccaa caatcaagct atgcggggag tatagttata tgcccggaaa agttcaagac
      961 ttctttctgt gctcgctcct tctgcgcatt gtaagtgcag gatggtgtga ctgatcttca
     1021 acaaacttat taccgccagg taaagaaccc gaatccggtg ttcacacccc gtgaaggtgc
     1081 ccggacgctt ccgttctgcg ggaaactgat ggaaaaggcg gtgggcttca cctcccgttt
     1141 tgatttcgcc attcatgtgg cacatgcccg ttcgcttggt ctgcgtcggc gcatgccacc
     1201 ggtactacgt cgccgggcca ttgatgcgct gctgcagggg ctgtgtttcc actatgaccc
     1261 gctggccaac cgcgttcagt gctccatcac cacgctggcc attgagtgcg gactggcgac
     1321 ggagtctgat gccggaacac tttccatcac ccgtgccacg cgtgccctga cgttcctggc
     1381 agagctgggg ctgattacct accagacgga atatgacccg cttatcggct gcaacattcc
     1441 gaccgacatc acgttcacac cggcgctgtt tgccgccctc gatgtatcag aggaggcggt
     1501 ggcgtccgcg cgccgcagtc gtgtggaatg ggaaaacaga cagcgcaaga agcagggact
     1561 ggataccctg ggtatggatg aactgatggc gaaagcctgg cgttttgtgc gtgagcgttt
     1621 ccgcagttac cagacagagc ttaagtcccg tggaatgaag cgtgcccgtg cgcgtcgtga
     1681 tgcggacagg cagcgtcagg atattgtcac cctggtgaaa cggcaactga cgcgtgaaat
     1741 ctcggaaggg cgcttcactg ccagtcgtga ggcggtaaaa cgtgaagtgg agcgtcgtgt
     1801 gaaggagcgc atgattctgt cacgtaaccg taattacagc cggctggcca cagcttcccc
     1861 ctgaaagtga cctcctcaga ataatccggc acgcgccgga ggcattcgca cgcctgaagc
     1921 ccgccagtgc acaaaaaatc agcgctacat acaaaaaaca acctcatcat ccaccttctg
     1981 gtgcatccgg tcccccctgt ttttgataca aaacacgcct cacagacggg gaattttgct
     2041 tatccacatt aaactgcaag ggacttcccc ataaggttac aaccgttcat g
//
LOCUS       DAZPAC010000403         2089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZPAC010000403 DAZPAC010000000
VERSION     DAZPAC010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.412"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHUPM_004557"
     CDS             complement(60..1025)
                     /locus_tag="ACHUPM_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR0872118.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQIAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHUPM_004558"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHUPM_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872119.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          549 c          437 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcaatc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag acttgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcactccc gaacggctga gcccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZPAC010000404         2089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZPAC010000404 DAZPAC010000000
VERSION     DAZPAC010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.413"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            41..1453
                     /gene="ospC1"
                     /locus_tag="ACHUPM_004559"
     CDS             41..1453
                     /gene="ospC1"
                     /locus_tag="ACHUPM_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFR0872120.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSIANQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(1557..1706)
                     /locus_tag="ACHUPM_004560"
                     /pseudo
     CDS             complement(1557..1706)
                     /locus_tag="ACHUPM_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1782..>2089
                     /locus_tag="ACHUPM_004561"
     CDS             1782..>2089
                     /locus_tag="ACHUPM_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR0872121.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     "
BASE COUNT          752 a          347 c          359 g          631 t
ORIGIN      
        1 aagatattaa ttaaaactgt tttcatataa ggttcatttt atgaatatat cagaaacact
       61 gaactcagca aatacccaat gcaatataga ttctatggat aacagattac atacattgtt
      121 tccaaaagtg acatcagtgc gaaacgctgc acaacaaact atgccagatg aaaaaaattt
      181 aaaagatagt gcaaatatta ttaaagattt ctttaggaaa actatagcag cacagagtta
      241 tagtagaatg ttctctcaag gctctaactt taaatcttta aatatagcaa ttgatgcacc
      301 atcagacgct aaagcctcat ttaaggctat tgagcacctt gacagattat cgaagcatta
      361 tatatctgaa ataagggaaa aacttcatcc tctttctgca gaggaactca atttgctttc
      421 gctaattatt aattctgatt taatcttcag acatcaaagt aattctgatt tgtctgataa
      481 aattttaaac attaagtcat tcaataaaat tcagtctgaa ggaatatgca caaaacgaaa
      541 cacatacgct gatgatataa aaaaaatagc taatcatgac tttgtgtttt ttggcgttga
      601 aatctctagc catcagaaaa aacaccccct gaatacaaaa catcacactg ttgattttgg
      661 tgcaaatgcg tatatcattg atcatgactc tccatatgga tatatgacat taaccgatca
      721 ctttgataat gctattccac ctgtttttta ccatgagcac caatcatttt tagataaatt
      781 ttcagaggtt aataaagaag ttagtcgata cgtacatgga agtaaaggaa ttatagatgt
      841 accaatattc aatactaaag atatgaagtt agggctcgga ttatacctga ttgactttat
      901 tagaaaaagt gaagaccaaa gcttcaagga gttttgctat ggaaaaaatc ttgcccctgt
      961 ggatctggat agaatcataa actttgtttt tcagccagag taccatatac ctaggatggt
     1021 aagtacagaa aacttcaaaa aagttaagat tagagaaata tccttagagg aggctgttac
     1081 agcatctaat tacgaagaaa ttaacaagca ggtcactaac aaaaaaattg ctctccaggc
     1141 tctttttctt tcgattgcta atcaaaaaga ggatgtcgcc ttatatatat tatctaattt
     1201 tgagataact agacaagatg ttatttccat aaagcatgag ttgtatgata ttgagtatct
     1261 acttagcgct cataattcaa gctgtaaagt acttgagtat tttatcaata agggattggt
     1321 tgatgtaaac acaaagttca aaaaaactaa tagtggggat tgtatgttgg ataacgcaat
     1381 aaaatatgag aatgcagaaa tgataaaact attattgaaa tatggtgcaa catctgacaa
     1441 taaatatatt taatcaaaat tgaatatcgt ttagcaaaag gcagtatttg cacaaaatat
     1501 cgatatattg tttattgtca gtatggctca atgtgataat agttggaaag tttgatgggt
     1561 ttcgccccgt tgtagcggta gtcgagctgg aaggtcttca ggcactgctt acagcgatag
     1621 agcagccccc ctgaactgga atggccgttc cggatacccc cctgagtttc agagtaacgg
     1681 ggacaaacca catcaatctt tgccatcaat catccaaagg gcaaagagta caacaacact
     1741 aagtctgcgt cacaacccat caatgaaagg aatatataca tatgccatca gtaaatttaa
     1801 ttccatcaag gaaaatatgt ttgcaaaata tgataaataa agacaacgtc tctgttgaga
     1861 caatccagtc tctattgcac tcaaaacaat tgccatattt ttctgacaag aggagttttt
     1921 tattaaatct aaattgccaa gttaccgatc actctggaag acttattgtc tgtcgacatt
     1981 tagcttccta ctggatagca cagtttaaca aaagtagtgg tcacgtggat tatcatcact
     2041 ttgcttttcc ggatgaaatt aaaaattatg tttcagtgag tgaagaaga
//
LOCUS       DAZPAC010000405         2073 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZPAC010000405 DAZPAC010000000
VERSION     DAZPAC010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.414"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            2..698
                     /locus_tag="ACHUPM_004562"
                     /pseudo
     CDS             2..698
                     /locus_tag="ACHUPM_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            705..1451
                     /locus_tag="ACHUPM_004563"
     CDS             705..1451
                     /locus_tag="ACHUPM_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0872122.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1466..1888
                     /locus_tag="ACHUPM_004564"
     CDS             1466..1888
                     /locus_tag="ACHUPM_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0872123.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1889..>2073
                     /locus_tag="ACHUPM_004565"
     CDS             1889..>2073
                     /locus_tag="ACHUPM_004565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872124.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPVKEDDPRNQ
                     VNFLYEPSERPYCHHASV"
BASE COUNT          568 a          456 c          604 g          445 t
ORIGIN      
        1 tattcgcttc tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc
       61 tcgcggcgtt acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc
      121 gttaattcag gttggatggc tggtcgaaaa tgacggaggg ctttctctac ctaactttga
      181 acgtcataac ggaaaaagct ctaaaaaaaa cgggcggtta caaacgagcg agtaacaaaa
      241 atacgcgaac tgaaacgaaa aggtaacgct gccagcgtta cacaaacgga tcaaaaagcg
      301 ttaccagagg aagaggaaga ggaagaggaa gaggaagagg aagaggaaga tctaaatact
      361 gatctccccc tcgccaaaaa cgagcgtcta aaaaattcga gccggaggct attgagctgc
      421 ccgattggtt gccggaaaca ctctggcatg agtgggtccg gttcagacag gcattgcgaa
      481 aaccgattcg aacggagcag ggcgctaacg gggcgatacg ggaactggaa aaattccgtc
      541 agcagggttt tacacctgag caggtgattc gacacagcat cgccaatgaa taccagggcc
      601 tgttcgcgcc gaaaggtgtt cggcctgaga cgttgctccg acaggttaac accgtctcgt
      661 tgccggacag tgcgatcccg ccaggcttca gggggtaacg gaccatgaaa aatattgcga
      721 caggcggcgt tctggaacgc atccgccgac tgaccccgcc acatgtaacc gccccattca
      781 gaacggtagc ggagtggcgc gagtggcaac ttgctgaagg ccagaaacgt agcgaggaga
      841 tcaaccgcct gaatcgccag ttgcgggtgg aaaaaattct gaatcgctca ggcatccagc
      901 cgttgcaccg taaatgctcg tttgcgaatt accaggtgca gaacgacggc cagcgatacg
      961 cgttaagcca ggcgaaatcc atcgccgatg aactgatgac cgggtgtaca aattttgcgt
     1021 tcagcggaaa acctggtacc gggaagaatc acttagcggc agctatcggg aatcgcctgc
     1081 tgaaagacgg tcagacagtg attgtggtta ccgtggctga tgttatgagc gccctgcacg
     1141 ccagctatga cgacgggcag tcaggcgaaa aatttttgcg ggagctgtgc gaagtggatc
     1201 tgctggttct tgatgaaatt ggcattcagc gcgagacgaa aaacgagcag gtggtgctgc
     1261 accagattgt tgatcgccgg acagcgtcga tgcgcagcgt ggggatgctg acaaacctga
     1321 actatgaggt gatgaaaaca ttgctcggcg agcgggtgat ggatcgcatg gtcatgaacg
     1381 gcgggcgctg ggtgaatttt aactgggaga gctggcgtcc gaatgttagc cattcgaggg
     1441 ttgttaagta gtttcaggag gatttatggc gaaacctttt actcccgaac agcgggaaga
     1501 actgaagacg cgaattgtgg aactcgtgca tcaggacggt cgggtcacga ttcggcagtt
     1561 gtcagatgaa acaggtatca gtcgtgcgtc tgtcggtcgc ttatgcatag aactggtcgc
     1621 aagtggtgat gtatataatt ctggctacgg cttattcccg tctgaacagg ctcgcaagga
     1681 ctggcaaagc gcccgcaaaa aactctcgag agtaaaggtg aggaaaccgg ttgttgttga
     1741 tccggacctt atctggtcat tacctgacgg agaaatacgc cgctacgaca ggcgcctgaa
     1801 tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg ctttctatca
     1861 gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc aattaccgct
     1921 ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt taagcaatat
     1981 cgttttattt ccggtaaaag aggatgaccc tcgtaatcag gttaattttc tttatgagcc
     2041 atcggaaaga ccatattgcc atcacgcctc tgt
//
LOCUS       DAZPAC010000406         2069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZPAC010000406 DAZPAC010000000
VERSION     DAZPAC010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.415"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..856
                     /gene="espL1"
                     /locus_tag="ACHUPM_004566"
                     /pseudo
     CDS             1..856
                     /gene="espL1"
                     /locus_tag="ACHUPM_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1028..1135
                     /gene="yniD"
                     /locus_tag="ACHUPM_004567"
     CDS             1028..1135
                     /gene="yniD"
                     /locus_tag="ACHUPM_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR0872125.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1188..1946)
                     /locus_tag="ACHUPM_004568"
     CDS             complement(1188..1946)
                     /locus_tag="ACHUPM_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR0872126.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          644 a          462 c          422 g          541 t
ORIGIN      
        1 acacgaatgc catttgcccc tatacatcca tcggcaatgt tgttaataat ggccacaggc
       61 cagactcaat agcttattac actattcaaa cagttgccca tactccctga aaaagaaatc
      121 attgaaataa taactgcgca gaatagcgtt ggtacacctg ctttatttct ggctatgatg
      181 aacggacaaa ctgacaacgt aaaaatattt atgcaagaaa ttcagtcact ggtatacaat
      241 catatcattc atgaagataa tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca
      301 cctggactct atatctccat gttgtatgga ttcgatgaaa taatcgatat ctttctgaat
      361 gcattaacca ctccaatagc acaagaactt ttaaacaaaa aaaatggtga tgagtatttt
      421 agccatgaaa atacatgatg gtgagccagg attatacgcc gcaatggaaa ataatcaccc
      481 tttgtgtgtc acacggttcc tctctaaaat taatggcatc gcctttaaat acaagttgag
      541 caaagctaac atcatggatt tattaaaagg agctacagca cagggaaccc ctgcattata
      601 catcgccatg agcaagggta atgaagacgt cgtgttatct tatatatcga cgctgggtgc
      661 ttttgcaaaa aaacattctt ttagtcaaca tcagttattt acactattgg ccgctaaaaa
      721 tcatgacaac atgtcagctg ttcatatagc cattcatcat aagcattata aaactgtaga
      781 aacatattat gctgctataa atgtcatcag ccaaagcctg agttttagtg ctgatgaaat
      841 aaagacgtat ttataaccag ctatttacga tatatatcga tacataattt tatttttttc
      901 ataaaacgca gaaacagtga ccatgtgcac aacgccaccc gacggctgta ataacaaaaa
      961 atcccttgat gcctatccct tttgttacac tccgttatca cgcacaagag atatgcagga
     1021 cactggtatg ccgactaaac gctttgataa aaaacactgg aagatggtgg tggtgctact
     1081 ggcaatctgt ggcgctatgt tgttgctacg ttgggcagca atgatttggg gctgagcagt
     1141 gacaaacgaa gacagccaga ccgcataaca cggtccggca gataatttta catgctgtag
     1201 cccagcgata aggttgtccg acgatcggta tgttccggtg cagactctgg aggttcagag
     1261 ttccatgtga cgttgtaagc caccttcagt ccaaaatgtt cattaatagc aacatttaat
     1321 gcgctttcgg agttcagcgt tgtatcttcc gcgccaaaga ccgaaacacc ctgcgtaaat
     1381 ttagcgttgt cagtcaactg ccatgcataa gccccggagg catagcccag cggctgggtt
     1441 tcactggcat tgtcggtata tttgtcgtaa cgcacacctg ggccgaattc aaagcggaaa
     1501 ctgtgtaccg ggccattcaa aaactggcga ccataacccg cggtcaacac atcgcgctct
     1561 cgatagccgt tataacggtc tgtcagccag cttgcctgac caaataaata gtcataatca
     1621 gttaaattaa aacggctacg cccgcccgcc gcatattttt ctgaagaacg ctcatcatta
     1681 gaagaagtat tactggcgtt cccccacagc gaccaggcag tggtttgtcc ataccaggtc
     1741 atggtggtgt cagccgtaag tgaggagctt ttcgtattgc ctgattgtgc aagatatcct
     1801 gcgttcagat taccttcgaa aggtttttta gcgctggcag gatcgtccat gacagtaaaa
     1861 acggaatcat cggcagctgc attcagtgac gcaaacatgc cccccgccaa cataacgatg
     1921 gcaggaactg tcttcaaaag cttcatttat caagagtccg tacaacaaaa aaagagacca
     1981 tcgcggtccc ggaaactttc ttaaggatca aagattagcg tccctggaaa ggtaacgaat
     2041 tataaaaagg cgcgaataac ttagcaatg
//
LOCUS       DAZPAC010000407         2048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZPAC010000407 DAZPAC010000000
VERSION     DAZPAC010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.416"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            18..527
                     /locus_tag="ACHUPM_004569"
     CDS             18..527
                     /locus_tag="ACHUPM_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR0872127.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            524..1942
                     /gene="phrB"
                     /locus_tag="ACHUPM_004570"
                     /pseudo
     CDS             524..1942
                     /gene="phrB"
                     /locus_tag="ACHUPM_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
BASE COUNT          486 a          505 c          566 g          491 t
ORIGIN      
        1 accgttcagg agcagtaatg aatctacaac gatttgatga cagcacccta atccgtatct
       61 ttgcccttca tgagttacat cgactgaaag aacatggctt aacgcgcggg gcgcttctcg
      121 attatcacag ccgctataaa ctcgtctttc tggcgcattc tcagccggag taccgcaaac
      181 ttggcccgtt cgtggctgat attcaccagt ggcaaaatct ggatgactat tacaaccagt
      241 accgccaacg cgtagttgtt ttgctttctc accccgccaa cccgcgcgat cacaccaatg
      301 ttttgatgca cgttcagggt tattttcgcc cgcatattga ttccacagaa cgccagcagc
      361 tggctgcgct tatcgacagt tatcgccgtg gcgagcaacc acttcttgcg ccgctgatgc
      421 gtatcaaaca ctatatggcg ctttatcatg acgcctggct ttcagggcag cgttatttcg
      481 aactttggcc gcgtgtgatt aacttgcgcc attcaggagt tttatgacta cccatctggt
      541 ctggtttcgc caagatttac gtctgcacga taatctcgca ctggctgccg cctgccgcaa
      601 ttcgtctgca cgcgtgctgg cgttgtatat cgctacacca cgccagtggg cgacgcataa
      661 catgtcgccg cgtcaggctg aacttatcaa tgctcaactg aatgggctac aaatagcgct
      721 tgcggaaaaa ggtattcctt tattgttccg tgaagtggat gactttgtcg ccagtgtcga
      781 aatagttaaa caggtgtgcg cggaaaacag cgttacccac ctgttttata actatcagta
      841 tgaagtgaat gagcgggcgc gggatgtgga agttgaaaga gcgctgcgta acgtggtgtg
      901 tgaaggattt gatgacagcg tgatcctgcc gcctggcgcg gtgatgaccg gtaatcacga
      961 gatgtacaaa gtctttacgc cttttaagaa tgcctggctg aaacggctgc gggaagggat
     1021 gccggagtgc gtcgctgcgc caaaagttcg tagtagcgga tcgatagagc cctcgccatc
     1081 cattacgctg aattatcctc gtcagtcttt cgatactgcg cattttccgg tggaagaaaa
     1141 agcggcgatt gcgcaattac gccagttttg ccagaacggt gccggagaat atgagcaaca
     1201 acgagatttt ccggcagtgg aaggcaccag ccgtttgtcg gccagcctgg caacgggcgg
     1261 gttatcgcct cgccagtgct tgcatcgctt gttggctgaa cagccgcagg cgctggacgg
     1321 tggggtcggt agtgtctggc ttaatgagct gatctggcgc gagttttacc gtcacctgat
     1381 aacgtatcac ccctcgttgt gtaaacatcg tccaattatt gcctagacgg atcgtgtaca
     1441 gtggcagagc aatcccgcac atttacaggc ctggcaggaa ggcaaaacgg gatacccgat
     1501 tgttgatgcc gctatgcgtc agcttaacag cactggctgg atgcataaca ggctacggat
     1561 gattacagcc agttttctgg tgaaagattt attgatcgac tggcgcgaag gcgagcgata
     1621 tttcatgtcg cagctgattg atggtgattt ggcagccaat aacggtggct ggcagtgggc
     1681 cgcttcaacc ggaaccgatg cagcgccgta ttttcgtatt ttcaacccga caacccaggg
     1741 cgagaaattt gatctcgaag gtgagtttat ccgccagtgg ctaccggaac tacgcaatgt
     1801 accggggaaa tcggtgcatg agccgtggaa gtgggcgcag aaagcaggtg tgaagctgga
     1861 ttatccgcaa ccgatagtcg agcacaaaga ggcgagagta caaacgttgg cagcgtatga
     1921 ggcggcgcgg aaggggaaat aacagacgcg tcaggcaatc gagcccagat gccggatgcg
     1981 gcgtgaacgc cttatccagc ctacaaattt gtgcaaattc aataaattgc aggaaatacg
     2041 taggcctg
//
LOCUS       DAZPAC010000408         2027 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZPAC010000408 DAZPAC010000000
VERSION     DAZPAC010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.417"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..711)
                     /gene="ipaJ"
                     /locus_tag="ACHUPM_004571"
     CDS             complement(<1..711)
                     /gene="ipaJ"
                     /locus_tag="ACHUPM_004571"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFR0872128.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARR"
     gene            complement(908..1717)
                     /locus_tag="ACHUPM_004572"
                     /pseudo
     CDS             complement(908..1717)
                     /locus_tag="ACHUPM_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          582 a          522 c          332 g          591 t
ORIGIN      
        1 tcttcttgca cctaattcca tagttgaaaa acaagaataa ttttctccta cagctggatc
       61 catatagctt ccatcaggtc tacacaacac ccagtggagg ccgacaggaa ctccaataaa
      121 ggatacagca acagcttcca aaactctttg gttagaagat aacacatctc gttgatgtac
      181 tatattacat cccatgccaa ttaataagtc ccttgcatcg ggataaataa aactaattac
      241 ttgcgaaaaa atgttacttt cttcaacgac ataagcattc aatccaagaa gcctcgtagc
      301 catgactatc ttatcaggca tagaataacc ggcatccgcg atattatctt tgtgtattct
      361 cggattataa ttaccactag ttattaagta taaatcccgc tcacaacgat tatccaaatc
      421 aagagaactc ttccttgtca tttcggacat agaccctttg tattgtggta ttttatcaac
      481 ccctaattcc tttgcagcac agagtaaact ggcagctcca caagaattgt catacatctg
      541 cttcctcgcc aaaaaacaac cttgttcgtt tactctattc tcatttaccc tcacatcagt
      601 ctgatgagat atcatatatt ctcttggtat agccccctgc aataaaaccc catataacat
      661 aacacctgta tgaatacatc cccgtttgca aggcttccgt tgttccgaca tcgacgagta
      721 attgcagcac agtattgcat ccagcaatgc gaactactat gagaattagc tgaccgggtc
      781 cttattcccc gaacgaactt taaatccaaa acctcatact cttttccgtc gtcatcacgt
      841 ccgtcaccat aacaaccttt cagcatccac ccttttatta agagttaatg tccatcagca
      901 tcagcaatta ataccgcctg atactgacat atttttgtcg tatttacgtc cataatcggt
      961 tattgtgatt caggtcacac cagcttattt gctattgtta caataaccac cttggttgag
     1021 agctgttttt tcaactttgt tatcgactcc cccaactcct tggtcagtaa ggctgccgac
     1081 cgagcacagt gaatagatag tatccgcagg taccaccatg cttacttaat actcagaagg
     1141 cgaagtgctc tccaattaac tctgccgcac tatgcttata agctgctttt caatgccgtc
     1201 tatcaacatg tccagttcat tcaatctgtt gtactggtaa tccaacgcag taatgatata
     1261 tgcaggtatt ctttcatcca ctccagtaac gtttcatgca aagcattaat ttacgcgcta
     1321 cggaacttca ccagttgact gagcaaacga tgtgggacca gtactgcctg cagttcttct
     1381 attgttacag cgatttcttt accaaacagc tgaaccacca tcaagataaa gatagcctga
     1441 acattcatcg caccattttg ttaccaatgg caaaagattt aacgaaccgt gcctgcatca
     1501 ggcggacttt ttatccctgc tttgttagct ccctaacctt gtacagagag cctccataag
     1561 ctcccatatc aagcagacat ggttcgcagt tactgacata caccagaaaa ctctgacagc
     1621 agactcactt atcctccact tcactcatat ccttattggt gagatgaacc tgaatgacat
     1681 gttttgcgac ataaacgcca accagtgtat atttcacctg tagagattct acaatctgta
     1741 gcgttatgca tctaatgtta ggctctatga tgtcagaaat ctgcgagact ctacaccttc
     1801 tgcatttacc tttatttatc accatgctcc tcagttaacg gagaacgtgt tcattgcatt
     1861 ttgtcactta catttcgcct ttggtaccgg ggggatatag gttccaacgt tccgcatgag
     1921 aacctgaacc ggcttcacgc caccttgata ccagatgccg cacaggcagt caacaagtgt
     1981 cacgaacggt gcaatagtga tccacaccca acgcctgaaa tcagatc
//
LOCUS       DAZPAC010000409         2013 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZPAC010000409 DAZPAC010000000
VERSION     DAZPAC010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.418"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(37..273)
                     /locus_tag="ACHUPM_004573"
     CDS             complement(37..273)
                     /locus_tag="ACHUPM_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR0872129.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            414..1202
                     /gene="fhuF"
                     /locus_tag="ACHUPM_004574"
     CDS             414..1202
                     /gene="fhuF"
                     /locus_tag="ACHUPM_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR0872130.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="ACHUPM_004575"
     CDS             complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="ACHUPM_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR0872131.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1875..>2013)
                     /locus_tag="ACHUPM_004576"
     CDS             complement(1875..>2013)
                     /locus_tag="ACHUPM_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFR0872132.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
BASE COUNT          527 a          530 c          439 g          517 t
ORIGIN      
        1 tatctttact tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattat
       61 catcagcatg atggcagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa
      121 ctgtttgtga tacagcatca ccggcgtcaa tactaatccc atcagaatga caatccgcca
      181 ctgatccggc gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac
      241 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      301 aaccaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
      361 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct
      421 atcgttccgc accgctctat gaagatatca tctggcgaac gcatctccag ccgcaggatg
      481 ctgggcttgc acaagccgta cgtgcgacga tcgccaaaca tcgtgaacat ttgctcgagt
      541 ttatccgcct ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcac
      601 cgaatgcgct aagttctcta ctcgcggtct attccgatca tatctatcgc aaccaaccaa
      661 cgatgatccg cgagaacaaa ccgctgatct cactatgggc acaattgtat atcggcctga
      721 tggtgccacc attaatgctg gcgctactga cgcaggaaaa ggcattagat gtgtcgccgg
      781 aacatttcca tgctgagttt cacgaaaccg gacgcgtcgc ctgtttctgg gtcgatgtgt
      841 gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaacg ttaatcagcc
      901 aggcgctggt tccggttgtg caggcactag aagcgaccgg agagatcaac ggcaaactta
      961 tctggagtaa taccggttat ttgatcaact ggtatctcac tgagatgaag caactgctcg
     1021 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg ctcacgaacg
     1081 gtgaagataa tccactctgg cgtaccgtcg tgctgcgcga tggcctgctg gtgcgccgaa
     1141 cctgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
     1201 gaaagcactg ccggggaagt aaacccggca tcatgcggat taatagggat gcaacacatt
     1261 acttgcttcg caatgccgca tacataacag aatatctgcg gcctccaaca accctgcatc
     1321 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
     1381 gcgtttaagc tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat
     1441 ttctctctcc gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg
     1501 gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
     1561 cgttgacgct ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg
     1621 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaaact taatcgccag
     1681 ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaaat
     1741 gaccgccgca aatgagatcc gcgacatggc atcctgaaat gactcctgac tggtataggt
     1801 atggagctta taagggttac ccgcaaactt tctgaataaa ctctcaatac cgatactgct
     1861 catgacgcat ttttctatga gtgcgacatt tctcttctta attcggctgt gttccattct
     1921 gcggcatctc cataaacaga aggcaagtta agttctcttg caccctgcgc gctatttatc
     1981 catgcataca tttcagcgtt gctgtgaatt gcc
//
LOCUS       DAZPAC010000410         2011 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZPAC010000410 DAZPAC010000000
VERSION     DAZPAC010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.419"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(133..603)
                     /gene="argR"
                     /locus_tag="ACHUPM_004577"
     CDS             complement(133..603)
                     /gene="argR"
                     /locus_tag="ACHUPM_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR0872133.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            1038..1976
                     /gene="mdh"
                     /locus_tag="ACHUPM_004578"
     CDS             1038..1976
                     /gene="mdh"
                     /locus_tag="ACHUPM_004578"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0872134.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT          521 a          438 c          519 g          533 t
ORIGIN      
        1 ccgtatgtat tcattgtgtg aatgatatgt cgcggtaaaa cgcactattc gttaaattaa
       61 tcgcagggga aagcaggggg ttgagaggaa taagcaacat tttccccgcc gccagaagcg
      121 acggggcaga gattaaagct cctggtcgaa cagctctaaa atcgcttcgt acaggtcttt
      181 gacggtgaaa ccgttagcag gggtggtaaa gatggtgtca tcgccagcga tggtgcccag
      241 aataccttct gctttgccca gtgagtccag caggcgagca attaactgcg ccgcgccagg
      301 gctggtatga atcacgacaa ctgcatcgtt gtagtcgata tccagcacca gattcttcaa
      361 tggactggag gtggttggta cacccagttc agctggcagg cagtaaacca tttccatttt
      421 ggcattgcgt gtacgtacag caccaaactt ggtcaacatc cgcgagactt tagactgatt
      481 aatattgtca aagccttgct cctgcaacgc ggcgacgatt tcgccctggg agctaaattt
      541 ctcttcttta agtaatgctt taaatgcttt aactagttct tcttgcttag ccgagcttcg
      601 cataagtcac ccgatatggt ggttgataca acattattgt gcatacagat gaatttttat
      661 gcaaacagtc agccctgaag aaggctgaaa taatgttatg aaagagcggg attttatcaa
      721 atttcgttat tgagaaacat gcctgcgtca cggcatgcaa attctgttta aaagtaaatt
      781 aattgttatc aaattgatgt tgttttggct gaacggtagg gtatattgtc accacctgtt
      841 ggaatgttgc gctaatgcat aagcgactgt taattacgta agttaggttc ctgattacgg
      901 caattaaatg cataaacgct aaacttgcgt gactacacat tcttgagatg tggtcattgt
      961 aaacggcaat tttgtggatt aaggtcgcgg cagcggagca acatatctta gtttatcaat
     1021 ataataagga gtttaggatg aaagtcgcag tcctcggcgc tgctggcggt attggccagg
     1081 cgcttgcact actgttaaaa acccaactgc cttcaggttc agaactctct ctgtatgata
     1141 tcgctccagt gactcccggt gtggctgtcg atctgagcca tatccctact gctgtgaaaa
     1201 tcaaaggttt ttctggtgaa gatgcgactc cggcgctgga aggcgcagat gtcgttctta
     1261 tctctgcagg tgtagcgcgt aaaccgggta tggatcgttc cgacctgttt aacgttaacg
     1321 ccggcatcgt gaaaaacctg gtacagcaag ttgcgaaaac ctgcccgaaa gcgtgcattg
     1381 gtattatcac taacccggtt aacaccacag ttgcgattgc tgctgaagtg ctgaaaaaag
     1441 ccggtgttta tgacaaaaac aaactgttcg gcgttaccac gctggacatc attcgttcca
     1501 acacttttgt tgcggaactg aaaggcaaac agccaggcga agttgaagtg ccggttattg
     1561 gcggtcactc tggtgttacc attctgccgc tgctgtcaca ggttcctggc gttagtttta
     1621 ccgagcagga agtggctgat ctgaccaaac gcatccagaa cgcgggtact gaagtggttg
     1681 aagcgaaagc cggtggcggg tctgcaaccc tgtctatggg ccaggcagct gcacgttttg
     1741 gtctgtctct ggttcgtgca ctgcagggcg aacaaggcgt tgtcgaatgt gcctacgttg
     1801 aaggcgatgg tcagtacgcc cgtttcttct ctcaaccgct gctgctgggt aaaaacggcg
     1861 tggaagagcg taaatctatc ggtaccctga gcgcatttga acagaacgcg ctggaaggta
     1921 tgctggatac actcaagaaa gatatcgcct tgggcgaaga gttcgttaat aagtaattga
     1981 ttagcgaata ataaaaaacc ggagcacaga c
//
LOCUS       DAZPAC010000411         2010 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZPAC010000411 DAZPAC010000000
VERSION     DAZPAC010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.420"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(177..1037)
                     /locus_tag="ACHUPM_004579"
     CDS             complement(177..1037)
                     /locus_tag="ACHUPM_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR0872135.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1220..1651)
                     /locus_tag="ACHUPM_004580"
                     /pseudo
     CDS             complement(1220..1651)
                     /locus_tag="ACHUPM_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1717..>2010
                     /locus_tag="ACHUPM_004581"
     CDS             1717..>2010
                     /locus_tag="ACHUPM_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR0872136.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSR"
BASE COUNT          500 a          517 c          465 g          528 t
ORIGIN      
        1 gccggacatc agcgctatct ctgctctcag gggtctgacg ctcagtggaa cgaaaactca
       61 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      121 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
      181 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
      241 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
      301 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
      361 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
      421 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
      481 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
      541 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
      601 agctccttcg gtcctccgat cgttgtcaga agtaagttgg cagcagtgtt atcactcatg
      661 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      721 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      781 tgcccggcgt caacacggga taataccgca ccacatagca gaactttaaa agtgctcatc
      841 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      901 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      961 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacga
     1021 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta ccagggttat
     1081 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1141 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     1201 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaatttta taaaccgtgg
     1261 agcgggcaat actgagctga tgagcaattt ccgttgcacc agtgcccttc tgatgaagcg
     1321 tcagcacgac gttcctgtcc acggtacgcc tgcggccaaa tttgattcct ttcagctttg
     1381 cttcctgtcg gccctcattc gtgcgttcta ggatcctccg gcgttcagcc tgtgccacag
     1441 ccgacaggat ggtgaccacc atttgcccca tatcaccgtc ggtactgatc ccgtcatcaa
     1501 tgaaccggac tgccacgccc tgagcgtcaa attcctttat cagttggatc atatcggcag
     1561 tgtcgcggcc aagacggtcg agcttcttaa ccagaatgac atcaccttcc tccaccttca
     1621 tcctcagcaa atccagccct tcccggtctg ttgggcactg ttgcaaatag tcggtggtga
     1681 taaacttatc atcccctttt gctgatggag ctgcacatga acccattcaa aggccggcat
     1741 tttcagcgtg acatcattct gtgggccgta cgctggtact gcaaatacgg catcagttac
     1801 cgtgagctgc aggagatgct ggctgaacgc ggagtgaatg tcgatcactc cacgatttac
     1861 cgctgggttc agcgttatgc gcctgaaatg gaaaaacggc tgcgctggta ctggcgtaac
     1921 ccttccgatc tttgcccgtg gcacatggat gaaacctacg tgaaggtcaa tggccgctgg
     1981 gcgtatctgt accgggccgt cgacagccgg
//
LOCUS       DAZPAC010000412         2005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZPAC010000412 DAZPAC010000000
VERSION     DAZPAC010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.421"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHUPM_004582"
     CDS             <1..389
                     /locus_tag="ACHUPM_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872137.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHUPM_004583"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHUPM_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR0872138.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="ACHUPM_004584"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="ACHUPM_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR0872139.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="ACHUPM_004585"
     tRNA            complement(1806..1881)
                     /locus_tag="ACHUPM_004585"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="ACHUPM_004586"
     tRNA            complement(1890..1966)
                     /locus_tag="ACHUPM_004586"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAZPAC010000413         2003 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZPAC010000413 DAZPAC010000000
VERSION     DAZPAC010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.422"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1517
                     /gene="tssI"
                     /locus_tag="ACHUPM_004587"
     CDS             <1..1517
                     /gene="tssI"
                     /locus_tag="ACHUPM_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR0872140.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQ
                     TVGVNLVETVGSNQIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQV
                     GLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKD
                     GSIFLNGTHIHLEGESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            1593..>2003
                     /locus_tag="ACHUPM_004588"
     CDS             1593..>2003
                     /locus_tag="ACHUPM_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR0872141.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFD
                     IRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRS"
BASE COUNT          495 a          502 c          628 g          378 t
ORIGIN      
        1 tctgcatcag ccagttccgc tacagcgcac aaatccgtcc ttcttccgtg gtgaccaaag
       61 actacacctt taaacgaccc ggctgggcag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacacagtat gaagtgtatg actaccccgg acgtttcaag ggtgctcacg
      181 ggcagaactt tgcccgctgg cagatggatg gctggcgcaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttctccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc gagcagccgc
      361 aggcggtgcc gggacgcagt ggttcaggaa ccacgctgaa taaccacttt gcggtaatac
      421 cggcagaccg gacatggcgg ccacagccgt tgctgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggat tcggtaacct tgcgataccg cgcgtgggtc
      661 aggaggtcat tgtggacttc ctcaacggcg atccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggta gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgtgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgccg aaaaaatagg taacaaccag gcgatcaccg
      961 ttaccaataa ccagatccag aacattggcg ttaatcagat acagacggtt ggtgtcaacc
     1021 tggtggaaac ggtgggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg
     1081 tggggatcat tcgtgcgctg acggtgggtg tggcttacca gacgacggta ggcggcatta
     1141 tgaatacctc ggtggcgttg ttgcagtcct cacaggtagg gctgcataaa tcactgatgg
     1201 tgggaatggg ctacagcgtc aatgtaggga ataacgtcac cttctcggtg ggcaagacga
     1261 tgaaggaaaa caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct
     1321 gtggtaaggc aaggctggtg ctgacgaagg acggaagcat atttctcaac ggtacgcaca
     1381 ttcatctgga aggggagtcg gatgtgaacg gtgatgcgcc agtgattaac tggaactgtg
     1441 gtgccacaca acctgtaccg gatgcgcctg tgccgaaaga tttacccccc ggaatgccgg
     1501 atatgcggca attttgagtc aggtgcaaaa aattaaaacg gaactggtgc agtgaccaga
     1561 tgaaccatat taaaagcaga aggaattgat tcatgggcgg cggaaaaccg gcggcgcggc
     1621 agggcgacat gacccgcaag ggactggata ttgtgcaggg ttcagcgggt gtgctgatag
     1681 gtgcgccgac gggagtggcc tgttcggtgt gtccgggagg gattacctat gctaacccgg
     1741 tgaacccggt gctgggtgcg aaggtgcttc cgggcgagac ggaccttgcg ctgccctgcc
     1801 cgctgccgtt tattcttttc cgcgcctaca gcagctaccg gaccagaaca cccgcgccgg
     1861 tgggggtgtt tggtcccggc tggaaagcgc ccttcgatat ccgcttacag atacgcgatg
     1921 aaggcctgat actcaacgac agcggcggca ggagcattca ctttgagccg ctgtttcccg
     1981 gcgagataag ctacagccgc agc
//
LOCUS       DAZPAC010000414         1997 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZPAC010000414 DAZPAC010000000
VERSION     DAZPAC010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.423"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHUPM_004589"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHUPM_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR0872142.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="ACHUPM_004590"
     CDS             complement(390..1178)
                     /locus_tag="ACHUPM_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR0872143.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="ACHUPM_004591"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="ACHUPM_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR0872144.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHUPM_004592"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHUPM_004592"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0872145.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT          503 a          528 c          460 g          506 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cggctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgt attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagt
//
LOCUS       DAZPAC010000415         1996 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZPAC010000415 DAZPAC010000000
VERSION     DAZPAC010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.424"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..689
                     /locus_tag="ACHUPM_004593"
     CDS             <1..689
                     /locus_tag="ACHUPM_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR0872146.1"
                     /translation="SQKDVFMTYRTTGLATYRNYDFFGLIEGLNFAAQYQGKNERTDN
                     SHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANPLNASGKNAE
                     LWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQFDFGLRPSLAY
                     LKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFTRAVDIRTDN
                     IVATGITYQF"
     gene            853..1335
                     /locus_tag="ACHUPM_004594"
                     /pseudo
     CDS             853..1335
                     /locus_tag="ACHUPM_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1450..>1996
                     /locus_tag="ACHUPM_004595"
     CDS             1450..>1996
                     /locus_tag="ACHUPM_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0872147.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT          668 a          412 c          344 g          572 t
ORIGIN      
        1 ggagtcagaa agatgtcttc atgacatacc gtaccaccgg tctagcaacc tatcgcaact
       61 acgatttctt tggcttaatt gaagggctga actttgccgc gcaatatcaa ggcaaaaatg
      121 aacgtactga caacagtcat ctttatggtg ctgactacac gcgtgccaac ggtgacggtt
      181 tcggtatctc ctcaacttat gtttatgatg gctttggtat cggtgcggta tataccaaat
      241 ccgatcggac aaatgcgcag gaaagagccg ctgctaatcc tctcaatgcc tccggtaaga
      301 atgcagaact atgggccaca ggtataaagt atgatgccaa caacatctac tttgcagcta
      361 attacgctga aacattaaac atgaccacct atggggatgg ttatatctct aacaaagcac
      421 aaagttttga agtggtggcg caatatcaat tcgacttcgg cttgcgccca tcactcgctt
      481 acctgaaatc gaaaggcaga gatctgggcc gctacggcga ccaggacatg attgagtata
      541 tcgacgttgg tgcgacgtat ttcttcaaca aaaatatgtc gacctatgtt gattataaaa
      601 tcaacctgat tgatgaaagc gactttaccc gtgccgtaga tattcggacc gataacatcg
      661 tcgctacggg cattacctat cagttctaac gtcctctcct gaccagccct tctctgggct
      721 ggtttttgat ttaagtgctg ctgtatgact ccttaagtaa aaatgagcaa tttttgatat
      781 ttctggtcat tcacctcaat taatattatt ccatatcctc cggggacatc ttactataat
      841 atttattgat gagtgattat gaccaatatt aatacagctt gtgtaaaaaa taatgccagt
      901 tatcaactta ataacgcatt acctaacaaa gaaaccatct ccagcaattt ttgtgaacga
      961 ctagaacaat ggggtaataa atcggttaat aatggtgaag aaagagcaat tgccgtggag
     1021 cgaattaaag aagcttacaa ttcgaacatg gcatctcttg atttgtcata tcttgatttg
     1081 agtgaattac cgcctatccc ctcgacagtt aatacgttaa atctggaaaa caattgtcta
     1141 acttgtcttg acttagctga caatgccagt ctcgttaata tcaacctcag cgttaataaa
     1201 attaacacga taacctttcc aaaagaatca aaactggaaa acatttatat tgatcataac
     1261 aatctggaaa gtttagattt aaaaaatcag cattcattgg ttaatctgga agcgcaaaat
     1321 aacaaactga caaaaattaa tatttctgat agttataaac tgaaatttct taatcttgat
     1381 tataacaagc tagcatcgct ggatgtctcc cggcaagaat ctctgattga gttaagtgcc
     1441 catcacaata tgatcactga ccttatatta cacaatcatc ccaggatgaa aacaatcact
     1501 ttaaacgaca accatattgc acatttaaac gccaaaaaca ctacaaaact ggaatattta
     1561 aacttaagca ataacaattt actgccaacc aatgacattg atcaactaat atcatcaaaa
     1621 catctttggc atgtattagt taacggcatc aacaatgatc cacttgcaca aatgcagtac
     1681 tggactgcag taagaaatat aattgatgac actaatgaag tgaccattga tttatcagga
     1741 cttaatttaa ccactcaacc accagggctg caaaacttca catctatcaa tcttgataat
     1801 aaccaactca cacattttga tgcaaccaac tacgatagac tcgtaaagct aagtctgaat
     1861 agtaatgctc ttgagtcaat aaattttcct caaggcagaa atgtaagtat tacacatata
     1921 tctatgaata ataatgctct cagaaatatt gatatagata ggctttcatc agttacttat
     1981 tttagtgcgg cacata
//
LOCUS       DAZPAC010000416         1985 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZPAC010000416 DAZPAC010000000
VERSION     DAZPAC010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.425"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..358
                     /locus_tag="ACHUPM_004596"
     CDS             <1..358
                     /locus_tag="ACHUPM_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0872148.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(398..1069)
                     /locus_tag="ACHUPM_004597"
     CDS             complement(398..1069)
                     /locus_tag="ACHUPM_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFR0872149.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHUPM_004598"
     CDS             complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHUPM_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR0872150.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1406..>1985)
                     /locus_tag="ACHUPM_004599"
     CDS             complement(1406..>1985)
                     /locus_tag="ACHUPM_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HFR0872151.1"
                     /translation="WALLTAMNQPKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITG
                     RTHMAKAIIIGMAVSAIGIFSYVQLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETG
                     WMYRAVEGQVHYWWVGLGNVIGSTILAYYWDDFAPALATDWDKINLLKTFGPMGGLLV
                     TYLLLFTALMLIIGWEKRFFRRAAPQTAKEIA"
BASE COUNT          488 a          537 c          517 g          443 t
ORIGIN      
        1 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
       61 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
      121 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      181 ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt
      241 aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
      301 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      361 gtagcaggga tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact
      421 ccgcgccctg gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt
      481 ttgctggcac acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc
      541 gcatccattc ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta
      601 ccagaccctg gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct
      661 caaatggtgt gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa
      721 aaaaaggctg ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga
      781 ttgcccgacc atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc
      841 gggcgttaat cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat
      901 ccagatgaag gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt
      961 tgtatctgcc aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa
     1021 gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct
     1081 gagatcatac tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca
     1141 atgttcccgc tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg
     1201 gtcggcccgt cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga
     1261 atattattga tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac
     1321 tgcggcatcg cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca
     1381 aggcggtaat caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac
     1441 ggcggaagaa gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat
     1501 atgtcaccag caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt
     1561 cggtggccag cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca
     1621 cattgcccag accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc
     1681 cggtttcgca gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg
     1741 cgtttggtcc cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc
     1801 cgatggcact caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga
     1861 tccacatatc gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa
     1921 agccgacgcc aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg
     1981 cccag
//
LOCUS       DAZPAC010000417         1953 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZPAC010000417 DAZPAC010000000
VERSION     DAZPAC010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.426"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHUPM_004600"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHUPM_004600"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR0872152.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="ACHUPM_004601"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="ACHUPM_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR0872153.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
BASE COUNT          589 a          439 c          396 g          529 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt ttt
//
LOCUS       DAZPAC010000418         1942 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZPAC010000418 DAZPAC010000000
VERSION     DAZPAC010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.427"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            286..855
                     /locus_tag="ACHUPM_004602"
     CDS             286..855
                     /locus_tag="ACHUPM_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0872154.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            1583..1756
                     /locus_tag="ACHUPM_004603"
     CDS             1583..1756
                     /locus_tag="ACHUPM_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR0872155.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
BASE COUNT          597 a          391 c          430 g          524 t
ORIGIN      
        1 aaaatcttcg aaaatatctg acagatgtct gtagctgcta agtacccgtc acaccagcta
       61 tgtgaccccc tttgcaaaaa agccaaatat cattgacttt ctcggtgaat tgctataaag
      121 gcatctatct aggtctttaa ctgtataaat acacatgaca acaaacgccc gttaagtgtc
      181 tatataaaaa ggtagctgta gctatagaca acaattgtga taattgattt aatggctata
      241 gacaagcaag acagaccttt gttgactata tagagagata agaacatgcg tacattcctt
      301 tatgctcgtg tatcagttga tgacctcacc acacagaacc aagtagaagc tgttaaacgt
      361 gctggttatg aagtcagacc ttcacgagta attgaggaag ttattagtgg ggctacacca
      421 gctatggaca gaccacaatt tgtaaagctg gtggataagc tggaagaagg tgattgtctt
      481 gtagtgacca agattgaccg cttaggaagg gataacattg acgtacagaa gaccgtcaca
      541 atgttacttg gtatgggtgt taagctggtc tgtctagatt tgcctgtcca agacctttca
      601 agtgctgaag gtaaacttat tcttcaacta ttcagtacct ttgcagagtt tgaacttaac
      661 cgcatcagag agcgaaccag agagggctta gaacgagcta aagcacaagg taaggtgttg
      721 ggtcgtcctg tagctaaaaa cactacagag gccgttttag agcataaggc taagggattg
      781 tcacaaagtc aggttgcaaa gcttctgaat ctctctgtga gaacggttag ccgtcactgg
      841 acaaagacac tgtaaaaaaa acttagtagc atcttcatag cctccttcat gggggctttt
      901 ttgtgtctgt aacatatggt gaccaaacat ctatatatcg ttatatagcc ctgtatatag
      961 cactatatat agtgtctgga gagggacgac taacaccctg catagttccc tgaaaagggg
     1021 cttcacagat tctctatata tttcaataag ttaggataaa aagggactat accgatactg
     1081 catagaatct gtgtaggatt gcatagtcat aggggttgga acaagcccaa ctacacctga
     1141 taaagcctgt tattaacagg tagttgaatg tacgtgttta gctatgtaaa agcaccagaa
     1201 ctttagccat ttctaaccac tcctcgtgtt atctctattc cgtagcgatt cgctaccact
     1261 tatttaaaga taagacgtcc ttttcagagt gcaaatttca caaccactta tttgatttat
     1321 aacaactttc acaagcacgt aattttgtcg caaaatgaca catttttatc tcatcgcgtt
     1381 tttttaatca taagagcggc ttatggataa ttattggaga tgatatctat tctcgctaag
     1441 aagctgttgc aggatattac caaacgcggg tctacccgcg tcagttcact aagcttagtc
     1501 ccacgtagcg aaaatatggc agccgccata cgccgcgtta attctatgca atatgatgtc
     1561 tatacccaga cggaggtcag taatggcaaa ccatcgaggc ggttccggca attttgcaga
     1621 agaccgcgaa agagcatcag aagcaggtaa aaaaggtgga cagcacagcg ggggtaattt
     1681 caagaatgac ccgcagcgcg catctgaagc aggtaaaaaa ggtggcaaga gcagtcacgg
     1741 caaaagcgac aactagccgg gctaatcaat gacgaatgca tttttgtctg tagctcgtca
     1801 aaaagccatc accgccggtt acccggtggt tgatactgat gacaaatgta agcttgcctg
     1861 atgcgcgatg cttatcaggc ctaccagaag attgcaatat attgaatttg cactgttttg
     1921 taggccggat aaggcgttta cg
//
LOCUS       DAZPAC010000419         1939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZPAC010000419 DAZPAC010000000
VERSION     DAZPAC010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.428"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHUPM_004604"
     CDS             <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHUPM_004604"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFR0872156.1"
                     /translation="FIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDK
                     YQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIG
                     FSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1506..>1939
                     /gene="yeaK"
                     /locus_tag="ACHUPM_004605"
     CDS             1506..>1939
                     /gene="yeaK"
                     /locus_tag="ACHUPM_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR0872157.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSV"
BASE COUNT          510 a          451 c          456 g          522 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgt
//
LOCUS       DAZPAC010000420         1899 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZPAC010000420 DAZPAC010000000
VERSION     DAZPAC010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.429"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(89..643)
                     /locus_tag="ACHUPM_004606"
     CDS             complement(89..643)
                     /locus_tag="ACHUPM_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR0872158.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(640..930)
                     /locus_tag="ACHUPM_004607"
     CDS             complement(640..930)
                     /locus_tag="ACHUPM_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR0872159.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(930..1529)
                     /locus_tag="ACHUPM_004608"
     CDS             complement(930..1529)
                     /locus_tag="ACHUPM_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR0872160.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1601..1813)
                     /locus_tag="ACHUPM_004609"
     CDS             complement(1601..1813)
                     /locus_tag="ACHUPM_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872161.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          472 a          501 c          387 g          539 t
ORIGIN      
        1 taaagcgggc tctttcaaat gtccatgtct gctattcgcc tcgcggtaca gctttgcgaa
       61 gcgtacaaga attgaagcag tttttacgtc aaaaagcaat aacttttttc tctataccaa
      121 aagccataac cattggtttg tacaaaataa attctgccac ctttagccaa tgctcaatgc
      181 gtctttcaca ggttcttaaa ctccatttcg ggtgtgcatc attcagcagt tcagccattt
      241 tgcgtttggt catcccccgc ccctcatacc gttgccgaag gacgctaatc aatcctggat
      301 gctctgccag cacctcactt atgacccgat caatacataa cgcctctgca tcagtacaat
      361 gcgccagcca gctcttttgc ttaccgttaa tcatatcccg caaaaaagcc tcaagttcag
      421 acttgttcag acctgctttt ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca
      481 gctttttaga ggtcaacatc aggttaaaca tattccccgt cttaccgcca ccaatatacg
      541 accagcgccc ccacatacgt agttttccct gaatccagac actttccagc gtggtgagac
      601 gaaggtgttc tccgcttttt cctgtattcg ttgggtaaat cataaatatc cctcctttct
      661 ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt cttctttggt
      721 gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg cacgagctac aggcaatcgc
      781 tgcctgcata tcgtgtggct ttatcgctgt tccgcacgtt cccgccagtc ggtaatgcgc
      841 cagcacagac gtttccggat cgtgattgca gtagccagga attctgacgg tgcacatctg
      901 cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagcagctg cgcggccaca
      961 ttttcgactt gttccggaga ggaaaattta cggaacagaa tccagttcca cagcacattc
     1021 agaacagatt tataaacctg ctgaaactcg gtttcgtcca tattcgcaaa agcgatggat
     1081 tttgcccgac gcccgcggct accgtccgga taaaaatgtt cggtgtaaaa tccagcctga
     1141 atggttaccc actcgcgaaa agcctcaaac gactttagca atgccgtatc ccgggttctg
     1201 cgtgtcgcaa ctgtattcag atattgctct gcggcttcgc tcagagctgg cgtatgttcc
     1261 cgacctactg attcgcacag gtaatcaacg aaaccagaca gcagttctcg ttcgcgaggc
     1321 gtgatcgccc caccgaccgg agtccagtaa tcgaatccca gttgcaggag tttgaaaaaa
     1381 cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca ctcacctgtt
     1441 ttgatttgat gcagaaaatc gcaactctcc ggcgtcgccg ggagaagtaa tccggaagag
     1501 gtttgtttga tcagttgtat atgcgccatc gtagttctcc gctggcgcag tagaatgggt
     1561 gttcagcccg ttatgtagta taccagaatt aatgccaata ctaacaggat gctctgactc
     1621 gcaattcatc cagcagttta tcatttccca taatgtcact taccctcatc ggtaaaaaaa
     1681 ttgcctttcg accattacga tacatcattg attttttggt ttcagggaag taatccattt
     1741 cgactataac tgacaggtca tcacgacgta tgactgcgta tttactacta aatagtttct
     1801 ttattttttc cacgatgcct ccaggtttat aagtacaaac ggttatatca acatagagac
     1861 aaaaggtgat gctgccaact tactgattta gtgtatgat
//
LOCUS       DAZPAC010000421         1897 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZPAC010000421 DAZPAC010000000
VERSION     DAZPAC010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.430"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            188..523
                     /locus_tag="ACHUPM_004610"
     CDS             188..523
                     /locus_tag="ACHUPM_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR0872162.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(804..935)
                     /locus_tag="ACHUPM_004611"
     CDS             complement(804..935)
                     /locus_tag="ACHUPM_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR0872163.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1435..1511)
                     /locus_tag="ACHUPM_004612"
     tRNA            complement(1435..1511)
                     /locus_tag="ACHUPM_004612"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1475..1477),aa:Arg,seq:tct)
     gene            complement(1612..1687)
                     /locus_tag="ACHUPM_004613"
     tRNA            complement(1612..1687)
                     /locus_tag="ACHUPM_004613"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1652..1654),aa:Ile,seq:cat)
     gene            complement(1790..>1897)
                     /locus_tag="ACHUPM_004614"
     CDS             complement(1790..>1897)
                     /locus_tag="ACHUPM_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0872164.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          616 a          471 c          335 g          475 t
ORIGIN      
        1 ccttaaataa aaacctgatt atcaagcaga tatgcatgga taaactacaa gacgagatat
       61 aaaccaccct gcatttaaat aaacaataaa caacatcaga aaaataattc tgctctatgg
      121 tttacattca aaaatatcat ttatactttt cagaacatca ccagcaaggc ataaacaagg
      181 aaactaaatg aagtggattg tgattgatac agttatccag ccatcatgcg gaatatcttt
      241 ttcagtcata tggagtaaaa taaaattaat aatctggtat caatcggatg ctttcttacc
      301 tcctgaaagt atatttacac tgactcacac aggcatcatg ctcaataaca aagtgctacc
      361 tgtaaccatt tacaacgtag taccattcaa taaaacattc tggaattcaa tcaaaaatag
      421 ccaggaatgc cctacaaata cagataacgt attgaatgaa tgctttaata accgttgcac
      481 tctgcaaata tgtccttatg gactaaaaca acaaagtcca taaggagttt actcacatct
      541 gacaaaatca atataaacag cccctccgga gaggggctgg agagtggcgc tatgtgccat
      601 tgcatggtgc cgggtgcctc ccggtgaatt cagtaccagc acctgaatcc gcgattatcc
      661 catataccta ctcgctgatt gcccctccgc acagggggat tcaccatgcc agtttctttt
      721 aacaaactcc ccgcaaacca gacaacagtc aaccgcctga attgtgaagt atttaaaaat
      781 ttctcccgct aactgatacc cggctaacag tctggcgttt tctttttcag caacgggaaa
      841 gcaacaacca ccacacccgc caccagcaca ccgtcagcca gcactgacat tatccggctg
      901 ctgcaatgcc attcacaaaa acagtaagca atcacttttt accgtaacag gtgataatcc
      961 agatatgtat ctaccccaga tgagtaatcc gaagttcatc cataccacag gtcctggcta
     1021 ttctgttgta ctcctgaaca agagcaaata attctgaatt agcaaccatg aactcatcgc
     1081 aaaccctctg tatagcatca ctattcagaa taataacgtc tcttcccgaa agacgatcag
     1141 gagtacagaa caaaactgtc aaacggctga aggcctttgc tcgtgctgca ttgactatat
     1201 caatacgctg cctaaggatg aaacaccccg acgcctcatc aatattcact ctacccacac
     1261 catatgaatg ataaatattt aatgctgaaa aaaccattag accgtataac aaacactcaa
     1321 tcaatactta acagaacttt tatttttgac aaacataaaa tattttcaac aatatcctga
     1381 gccaggtata tttcagtata aggctctgcc ggaaggaatc tggaagaatg aatatggcgc
     1441 gctgtactgg attcgaacca gtgaccgatt gcttagaagg caattgctct gtccggctga
     1501 gctaacaacg ctgaataccg ataatggacc gccatcgggg acccgccccc gcaccaacaa
     1561 ccctgttatc gtgtcgtctg ctcttcctga taagctaatg gcggtttgtg atggtggccc
     1621 ttgctggatt tgaaccagcg acttggcgat tatgagtcgc tcgctctcac cactgagcta
     1681 aagggccggg agcagaataa taacggtccg taattaattc cgcaataaaa aacccgctcg
     1741 gcggcgggtt gtagaaactc ttctaacgtc aggcataaaa cgcccatagt tatgacgaat
     1801 ttaccacaga ttccggaaaa atcaacctag ttaccttttt taactgccgc tcagcccatg
     1861 cttcttcaat atcaaaccga gtcaccagcg catcata
//
LOCUS       DAZPAC010000422         1877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZPAC010000422 DAZPAC010000000
VERSION     DAZPAC010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.431"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(50..184)
                     /locus_tag="ACHUPM_004615"
                     /pseudo
     CDS             complement(50..184)
                     /locus_tag="ACHUPM_004615"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(197..1018)
                     /locus_tag="ACHUPM_004616"
     CDS             complement(197..1018)
                     /locus_tag="ACHUPM_004616"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0872165.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNESILLNGKTIRKLMKELGISSLIRRKKYRTYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1007..1877
                     /locus_tag="ACHUPM_004617"
                     /pseudo
     CDS             1007..1877
                     /locus_tag="ACHUPM_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1287..1403
                     /locus_tag="ACHUPM_004617"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          469 a          414 c          455 g          539 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 taaggttata tgagactatt tctccattat acaggtccat gattggtgat aaatagagct
      481 tcctgccatc gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag
      541 cataaaattg tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac
      601 ggtacttctt gcgcctgata agtgagctga tacccagttc cttcattaat tttctgatgg
      661 ttttaccatt aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat
      721 aacgcccttt atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat
      781 ctggcgcatt aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa
      841 ccagtaattg tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt
      901 ttgttccaat cgtaattttt cctgagctaa ggctttcagc ttttttagat aatgaaccgc
      961 cccgggaatc ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac
     1021 tcgttttccc ccgaagtccg tcaacgggca gtccgtatgg ttctggaaag tcagggcgaa
     1081 tatgactcac aatgggcggt aatttgttcc attactccaa agattggctg tacaccggag
     1141 actctgcgtg tctgggttcg ccagcatgag cgggatacca ggggcggtga tggagggctc
     1201 accaccgctg aacgtcagcg tctgaaagag ctggagcgtg agaatcgtga actgcgctgc
     1261 agtaacgata tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc
     1321 tggaaaaaat aatgccactg ctggataagc tgcgtgggca gtacggggtc ggaccggtat
     1381 gcagtgaact gcatattgcc ccgtcaacgt attaccactg tcagcaacag gacatcatcc
     1441 tgataaacgc agtgcccgtg cgcagcacga tgactggctg aagagagaga tacagcgcgt
     1501 atacgatgaa aatcatcagg tgtacggtgt gcgtaaagtc tggcgccagt tgttacggga
     1561 aggaatcagg gtggccagat gtacagtggc gcgcctcatg gcggttatgg gacttgtcgg
     1621 tgttctccgg ggtaaaaagg tccgcactac cgtcagccgg aaaaccgttg ccgcaggtga
     1681 ccgcgtaaac cgtcagttcg tggcagaacg tcctgaccag ctgtgggtgg ctgattttac
     1741 ttacgtcagc acatggcagg gcttcgtcta tgtggcgttc atcattgatg tgtttgccgg
     1801 atacatcgtg tggtgacgag tctcatcgtc tatggaaacg acattcgtgc tggatgcact
     1861 ggagcaggcg ttgtggg
//
LOCUS       DAZPAC010000423         1860 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZPAC010000423 DAZPAC010000000
VERSION     DAZPAC010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.432"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            123..371
                     /locus_tag="ACHUPM_004618"
     CDS             123..371
                     /locus_tag="ACHUPM_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR0872166.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            616..690
                     /gene="tap"
                     /locus_tag="ACHUPM_004619"
     CDS             616..690
                     /gene="tap"
                     /locus_tag="ACHUPM_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR0872167.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            683..1540
                     /gene="repA"
                     /locus_tag="ACHUPM_004620"
     CDS             683..1540
                     /gene="repA"
                     /locus_tag="ACHUPM_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR0872168.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          469 a          476 c          479 g          436 t
ORIGIN      
        1 acagtaatcc ccgttgattt gtgcgccaac acagatcttc gtcacaattc tcaagtcgct
       61 gatttcaaaa aactgtagta tcctctgcga aacgatccct gtttgagtat tgaggaggcg
      121 agatgtcgca gacagaaaat gcagtgactt cctcatcagg tacaaagcga gcatacagaa
      181 aaggtaatcc tctgacgctt gcagaacgtc agcaggcatc cctggcaaga aaacgagcta
      241 cacataaaga actcagagtg ttcattcctg ctgcgttaaa agcgcaattg caggtgatgt
      301 gtgaagctga gggcgttacc caggctgaga tgattgccga actgataaag cagaagagtg
      361 cttttagcta actaaaatgt agcgacatca cattcttgcc tgtttctggt gtgcgtgata
      421 gattgctgat cgtttaagga attttgtggc tggccacgcc gtaaggtggc agggaactgg
      481 ttctgatgtg gatttacagg agccagaaaa gtgaaaaccc cgataatctt cgacaagttt
      541 ggcgactgag aagattaccg gggccatcta aaaaccgtat agccaacaat tcagctatgc
      601 ggggagtata gttatatgcc cggaaaagtt caagacttct ttctgtgctc actccttctg
      661 cgcattgtaa gtgcaggatg gtgtgactga tcttcaacaa acgtattacc gccaggtaaa
      721 gaacccgaat ccggtgttta caccccgtaa aggtgccgga acgctgaagt tctgcgaaaa
      781 actgatggaa aaggcggtgg gcttcacttc ccgttttgat ttcgccattc atgtggcgca
      841 tgcccgttcg cgtggtctgc gtcgacgcat gccaccggtg ctgcgtcgac gggctattga
      901 tgcgctcctg caggggctgt gtttccacta tgacccgctg gccaaccgcg tccagtgctc
      961 catcaccacg ctggccattg agtgcggact ggcgacggag tctgctgccg gaaaactctc
     1021 catcacccgt gccacccggg ccctgacgtt cctgtcagag ctgggactga ttacctacca
     1081 gacggaatat gacccgctta tcgggtgcta cattccgacc gatatcacgt tcacatctgc
     1141 actgtttgct gccctcgatg tatcagagga ggcagcggcc gccgcgcgcc gcagccgtgt
     1201 ggtatgggaa aacaaacaac gcaaaaagca ggggctggat accctgggca tggatgaact
     1261 gatagcgaaa gcctggcgtt ttgttcgtga gcgttttcgc agttatcaga cagagcttaa
     1321 gtcccgggga ataaagcgtg cccgtgcgcg tcgtgatgcg gacagggaac gtcaggatat
     1381 tgtcaccctg gtgaaacggc agctgacgcg cgaaatcgcg gaagggcgct tcactgccaa
     1441 tcgtgaggcg gtaaaacgcg aagttgagcg tcgtgtgaag gagcgcatga ttctgtcacg
     1501 taaccgtaat tacagccggc tggccacagc ttccccctga aagtgacctc ctctgaataa
     1561 tccggcccgc gccggaggca ttcgcacgcc tgaagcccgc cggtgcacaa aaaaacagcg
     1621 tcgcatgcaa aaaacaatct catcatccac cttctggagc atccgattcc ccctgttttt
     1681 aatacaaaat acgcctcagc gacggggaat tttgcttatc cacatttaac tgcaagggac
     1741 ttccccataa ggttacaacc gttcatgtca taaagcgcca gccgccagtc ttacagggtg
     1801 caatgtatct tttaaacacc tgtttatatc tcctttaaac tacttaatta cattcattta
//
LOCUS       DAZPAC010000424         1822 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZPAC010000424 DAZPAC010000000
VERSION     DAZPAC010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.433"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..224)
                     /locus_tag="ACHUPM_004621"
     CDS             complement(<1..224)
                     /locus_tag="ACHUPM_004621"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872169.1"
                     /translation="MRSRRCRNRGGSVVLENGEQKDARDFEAAKEQCRIDNQLEIACN
                     VLWIVRLPLALYGHHFFRYLSNSITGAGFF"
     gene            1040..1573
                     /locus_tag="ACHUPM_004622"
     CDS             1040..1573
                     /locus_tag="ACHUPM_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HFR0872170.1"
                     /translation="MLEQQFEDFEQQKRRDHQAQDQSKSAKQKRDRLNPPDWSQVTKA
                     GWNMLDSISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDL
                     ESIGAIVKIPVGIYYAYALDPYQVWRGYNKSKKYAAFNAKTLTQKDGVIDRKLRLMLS
                     RREEFEGSLKTSNHDLD"
     gene            1586..>1822
                     /locus_tag="ACHUPM_004623"
     CDS             1586..>1822
                     /locus_tag="ACHUPM_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872171.1"
                     /translation="MRIKYESRWKLEELKLEICRSIDSLIDEYHIEYAMGINLYMNCE
                     DKEFNPVDIVNEDGKNIMFVVSNNEKIQKTKKSAK"
BASE COUNT          557 a          373 c          437 g          455 t
ORIGIN      
        1 aaaaaaaatc ctgcacctgt aattgagtta gataaatatc ggaaaaagtg atggccatac
       61 agagccagcg gcaggcggac aatccacaga acattgcatg caatttctag ttgattgtct
      121 atcctgcatt gctcttttgc cgcctcaaaa tcccttgcgt ctttttgctc cccgttttcc
      181 agaacgaccg agccgccacg gttccggcag cgccttgagc gcaaccggaa accgtctggg
      241 gagaacccca gacccccatc gctgtaacat cgacggtcta cgaccaccta ccaaccgcgt
      301 ggctgcctat tccgtgcaaa tgggctttgc agggggctga cgcggttcct ggcgacctga
      361 aatcccctgg ttaggggtat cggcgcgcct gacctcctaa aaccctctgt aagcgttttt
      421 cgcgccttcc atgccctgat gggcatttaa aacccgtttc gtgcgtctga gagcttctga
      481 gagcgtttga agagctatct ggcgactcat ccccgaaaga acaacagcgg aatcaccgat
      541 aacatgcgag aggtggcacc gctcgccgca gccccagcga ccgaacgtta gtgagcgagt
      601 gggcgaggaa gcggaagaga accggaaacc acattgagca cgtacgcact gatgcggggc
      661 gttttagcgg tgaacggact gcaaaaacat gatcgagctg ttgaacgtgg agagcgcgaa
      721 atcgggcaaa atcacggctg acgaggcttg cagaagagta agccagtata cactccgcta
      781 tcgctacgtg actgggtcag ggcttcgccc cgacacccca aatgttcgat gaactaatta
      841 aatcttgtgt agcaaaaaag tgctttgata gatctttaga gcaaaaatcg acattctctt
      901 aaaaatcaaa gcattatggc accaacatac gaaacgttat ttgcatgtgt atacgcaaac
      961 atgtttgcgt gtgttgtttc gtaatgtacg atataaattt cgtaaggtac gaaacaacgt
     1021 tgcaggtaag gatgcaagta tgctggaaca gcaatttgaa gatttcgaac agcaaaaacg
     1081 gcgagatcat caggcacaag atcaatccaa aagtgctaag caaaaacgag atcggttgaa
     1141 tccacctgac tggtcgcagg ttactaaggc gggctggaat atgttggact ctatatctga
     1201 gaaggttccc agagcattta ggctatattc tttttttgct caaaatatag ataaatcgtg
     1261 tggggctgtt gtatgtgacc aaaaattctt agctgattat ttcggtgtta gtttaaggac
     1321 gatccaatat tggattggag atttagaaag tattggtgcc atcgtgaaaa taccagtagg
     1381 tatctattat gcttatgcgt tagatccgta tcaggtatgg cgcggatata ataaatctaa
     1441 aaaatatgca gcgtttaacg caaaaacatt aactcaaaaa gatggcgtta ttgatcgcaa
     1501 gttgcggctc atgctctcta ggagagaaga gtttgaaggc tcgcttaaga catctaatca
     1561 tgacttggac taggtatgat gcgctatgag aattaaatat gaatcacgtt ggaaattaga
     1621 agaactaaaa ttagaaatat gtagatcaat agattcgtta attgatgaat atcatatcga
     1681 atatgcaatg ggaataaatc tatatatgaa ttgtgaagat aaggagttta atcccgttga
     1741 tattgtcaat gaagatggaa agaatattat gtttgttgtt agcaacaatg aaaaaataca
     1801 aaaaacaaaa aaaagtgcga ag
//
LOCUS       DAZPAC010000425         1818 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZPAC010000425 DAZPAC010000000
VERSION     DAZPAC010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.434"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(8..859)
                     /gene="hchA"
                     /locus_tag="ACHUPM_004624"
     CDS             complement(8..859)
                     /gene="hchA"
                     /locus_tag="ACHUPM_004624"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR0872172.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            1006..1416
                     /locus_tag="ACHUPM_004625"
     CDS             1006..1416
                     /locus_tag="ACHUPM_004625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872173.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            complement(1450..>1818)
                     /locus_tag="ACHUPM_004626"
     CDS             complement(1450..>1818)
                     /locus_tag="ACHUPM_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR0872174.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT          478 a          370 c          445 g          525 t
ORIGIN      
        1 acagggatta atccgcataa gctgccagca tttcctgcgc cgccagttta cccaacgcat
       61 tcgctgcgaa aggactgtcg ccggtgagaa ctttacggtc cttatgtact cgcccggtga
      121 tgtcgtcatt aataatgttc atgcccattt tcttcagctc ttcgccgaag taccaggtga
      181 gatgacccgg catatagcct atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc
      241 aaatggaata accattcagt gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc
      301 cgtggcaaag ggagataaca aaacggtcat ttttgattgc ccactgcaaa gcagcagcta
      361 cgtcctggct ttctggtaaa ccaataagtg cgccatgacc accaggaaca aagattgctg
      421 catattcgct atcagcgttg aggctggcga caacatccgc gagtttcttc ggattgcgga
      481 acaacgattt atgctgctca aagaatggca ttactttttc atctttgtgc ggcatagccc
      541 agtattcaaa cttggtcatc agaccggaaa tggtcgccac ttcgaattcg aaaccagcag
      601 catggagatg atacaacggc agcaacgttt caatcggatg gttaccggtc gagaacagtt
      661 ttccgttatc ggttggcaaa taacgttcgt ccgcggcgat caccagaatt ttatgcttac
      721 cgcgatacgg ttttggatag tccacgccat caagatcaga gacgggactg gtatattggc
      781 taagcgaata ttctgaaggg aagaatgcat tatcttcagc aatatcgacc tgcggatttt
      841 tacttgtttg aacagtcata gtgtattcct tattgttgct tagttagggt agtcactata
      901 tttgcgactg agctggtcgc tatttttaac cagtctggaa aattgagtca atatatcgaa
      961 agcggtagtc ggcgggtgta agtatgtaat gatgatgctg cactggtgtt gaatattatt
     1021 cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg gcctcaatga ttttaggcca
     1081 ttatcaaagg tacggggcgg cggggagaga tttagtgctg tgaaatattg tggtactgat
     1141 gaatatttat atcgcggatt ggtagagaaa agattatttc atgcactatt tttgcaaata
     1201 tttctatatg ggaagcgtgt ttgttacgga ttaattcata aagaaatgca tttgggtgtt
     1261 ggtttgaaga gtcgctggtt gatgttattg tgccaggagg ggcagggtaa ggtgctgtat
     1321 cgatatgcat tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaagcct
     1381 gaattcgatg agggacgaac tcaggctagt gtttagtttt acagtaacac gccgcagcac
     1441 gttggctgat tagaactggt agactaagcc cacaccaaca acgtcatctg ttgcgatgcc
     1501 attgttgtca tagaaatcgt catctttatc caacaagttt attttatagt caacatatgt
     1561 tgagaagttc ttattgaaat agtaggtcgc tccaatttca acatagttga ccagatcctg
     1621 atcaacatac tcctgatgac cattgttgtt atagtcgtta ccgccgaggt ctttgccttt
     1681 tgaataaaca tatgcaagag atgggcgcag accaaaatcg aactgatact gtgctacagc
     1741 ttcaaagttc tgggttttat ttgcgatacc cacattacca tatggtgtca tattgcgggt
     1801 ttcagcatac atcattgc
//
LOCUS       DAZPAC010000426         1802 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZPAC010000426 DAZPAC010000000
VERSION     DAZPAC010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.436"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..256
                     /locus_tag="ACHUPM_004627"
     CDS             <1..256
                     /locus_tag="ACHUPM_004627"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR0872175.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGTHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            315..1772
                     /gene="dtpC"
                     /locus_tag="ACHUPM_004628"
     CDS             315..1772
                     /gene="dtpC"
                     /locus_tag="ACHUPM_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFR0872176.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
BASE COUNT          388 a          430 c          475 g          509 t
ORIGIN      
        1 gaccgaagaa gtttacctcg acgaaatggt aggtcgtatt aacgccaata tgatccttcc
       61 gtatccgccg ggagttcctc tggtaatgcc gggtgaaatg atcaccgaag aaagccgtcc
      121 ggttctggag ttcctgcaga tgctgtgtga aatcggcact cactatccgg gatttgaaac
      181 cgatattcac ggtgcatacc gtcaggctga tggccgctat accgttaagg tattgaaaga
      241 agaaagcaaa aaataattag ctcgtacaag ggaagtggct tgccacttcc cttttttgct
      301 cataaggaga acacatgaaa acaccctcac agccgcgcgc gatatactat atcgtggcga
      361 tccaaatctg ggagtacttc agtttttacg gcatgcgtgc cttactcatt ctctatctca
      421 cccatcagct tggttttgat gataaccatg ccatcagcct gttcagcgca tatgcttctc
      481 tggtttacgt tacccctatt ctcggcggct ggcttgccga ccgcctgctc ggcaaccgca
      541 ctgcagtgat tgccggcgcg ctgttaatga cccttggcca tgtggtgctg ggcattgata
      601 caaattcaac ctttagcctg tatctggcgc tggcaatcat tatttgtggc tacggtttat
      661 tcaaatcaaa catcagctgt ttgcttggcg agctctacga cgagaacgat catcgacgtg
      721 atggcggttt ttcgctgctg tatgctgcgg gcaatatcgg ttctatcgcc gccccaatcg
      781 cctgcggcct ggctgctcag tggtatggat ggcatgttgg ctttgccctt gcgggtggcg
      841 gcatgtttat cggtttgttg attttcttaa gcggtcatcg tcatttccag tccacgcgta
      901 gtatggataa aaaagcgctc actagcgtca aatttgcctt accagtatgg agctggttag
      961 tggtgatgct ctgtttagcc ccagtatttt ttaccctgct gctggagaac gactggtccg
     1021 gatatttgct ggcgatcgtt tgcctcattg ccgcacaaat cattgcccgc atgatgatca
     1081 aattccccga acaccgccgt gctctttggc aaattgtatt gttgatgttt gtcgggacgt
     1141 tgttctgggt actggcacaa cagggcggca gtaccatcag cttgtttatc gatcgctttg
     1201 tgaatcgtca ggcattcaat attgaagtac ctacagcact attccagtcg gtgaatgcaa
     1261 ttgcagtgat gctcgctggg gttgtactgg cctggctggc gtcgccagaa agccgcggca
     1321 actcaacatt gcgcgtctgg ctgaagtttg cctttggctt actgctgatg gcttgtggct
     1381 ttatgttgtt ggcatttgat gcccgacatg cagcggctga cggtcaagcg tcaatgggcg
     1441 tgatgatatc cgggttggcg ctaatgggct ttgcagaact ctttattgac ccggtggcga
     1501 ttgcgcaaat cacgcgtctg aaaatgtctg gcgtattaac cggtatttat atgctggcaa
     1561 caggcgcggt cgccaactgg ctggcaggcg tcgtggcaca gcagacgaca gagtcgcaaa
     1621 ttagcggtat ggtaattgca gcttaccagc gattcttttc tcagatggga gagtggacgt
     1681 tggcttgtgt cgcgatcatc gtcgtattgg cctttgctac ccgttttctg tttagcacgc
     1741 cgacgaatat gatacaggag agcaacgatt aactgtcgta aaaacaattg gttatgaata
     1801 aa
//
LOCUS       DAZPAC010000427         1799 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZPAC010000427 DAZPAC010000000
VERSION     DAZPAC010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.437"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..597)
                     /gene="spa29"
                     /locus_tag="ACHUPM_004629"
                     /gene_synonym="spaR"
     CDS             complement(<1..597)
                     /gene="spa29"
                     /locus_tag="ACHUPM_004629"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFR0872177.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVL"
     gene            complement(597..857)
                     /gene="spaQ"
                     /locus_tag="ACHUPM_004630"
     CDS             complement(597..857)
                     /gene="spaQ"
                     /locus_tag="ACHUPM_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFR0872178.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            complement(872..1522)
                     /gene="spa24"
                     /locus_tag="ACHUPM_004631"
                     /gene_synonym="spaP"
     CDS             complement(872..1522)
                     /gene="spa24"
                     /locus_tag="ACHUPM_004631"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFR0872179.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            complement(1527..>1799)
                     /gene="spa33"
                     /locus_tag="ACHUPM_004632"
                     /gene_synonym="spaO"
     CDS             complement(1527..>1799)
                     /gene="spa33"
                     /locus_tag="ACHUPM_004632"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFR0872180.1"
                     /translation="ESNGESEHTEVSLALFNYDDINVKVDFILLEKNMTINELKMYVE
                     NELFKFPDDIVKHVNIKVNGSLVGHGELVSIEDGYGIEISSWMVKE"
BASE COUNT          710 a          345 c          267 g          477 t
ORIGIN      
        1 taatacttct gatagtaaca atactatcat aacaggactg gctaaaataa cagcctgact
       61 tgccaataaa gtcagaaatg ttaagatatt tgagacacgg aaagaacatt gagaaaataa
      121 cgggcatata ttataagaca attgtatgga ttctaaaata aagaccatac caccactgta
      181 tagaaataca actgcagaaa aaagattgaa aaattttgcc aactcagacg tatcaacacc
      241 attggcagga tcaattgaac tactaagcgt tgccccacgc tggttgtcaa taatgctacc
      301 aacagcatga aatatccaaa agggaagaga caagcaaaaa gagagaagga ggccaacaat
      361 tatttccttg aacataagga aataaacatg ttcaaaaaca gaagaaccta tgtctacctt
      421 accagaagta attaatcctg atgcaacaag aaaaatcaca ggtattctaa tagatggaga
      481 aattataccg ttatttaaaa atggaagaaa gaaaaataat ggagccaatc taaaaaaaac
      541 gccgttcagg agtattaaaa acacatgaat actttcgaac cagcttgaaa tgtccatcaa
      601 accccactct taattaaaaa cattatttca tgacaaaaag acaataaaac ctcaccatac
      661 catccagaaa gaagtagcaa agatattgag acacctataa gctttatacc aaaaggaagt
      721 gtctgctctt ggagttgagt cactgtctgt aacaaaccaa tagataatcc aataaccgta
      781 gctataccta ctggccataa ggaaaagata aggattaaat aaagagcctt attacccata
      841 taaactatat cagacacatt acctccatca tctaagcagg aacattgata tattgttcaa
      901 tcaaggcctt agacaggata ccccacccat caagcgcaac aaataataca agttttattg
      961 gtactgatat tgtgattgga ctcatcatca tcataccaag tgcaagtaaa atgcttgaaa
     1021 taacaagatc aacaacaaca aatggcaaat ataaataaaa acctattttg aacgcatctt
     1081 tgatttcact tagcgcatat gcaggcaaga gggaaaacaa tgaataatca ttattctctg
     1141 cactctttaa atcagcattt tcttcttcag acctttggaa aaaacgtgct aactcaagat
     1201 ctgtatgctt tttcaagtat tgcttatatt ccatcaagcc agaatcagaa aaacggacaa
     1261 tatcagatat tgtatcaaat ttttgagggc catttagata attttcataa ccagcttcaa
     1321 taatgggttt catcacgaac aaagccataa tcaatgcaat accattgaga gtcatatttg
     1381 atggaacctg ctgcaaacca agtgcatttc gtaccattac aaaaacaata gaaaacttta
     1441 tataacaggt accagcagca actaaaaatg gtaatagcgt aaaaaaagaa agtgtagcga
     1501 tgagggacat gtcactcagc atgagattac tcctttacca tccaagaact aatctcgata
     1561 ccataaccat cctcaataga aacaagttcc ccatgcccaa ccaaagaacc atttacttta
     1621 atatttacat gtttaactat gtcatcggga aacttgaata attcgttttc tacatacatt
     1681 tttagttcat tgattgtcat atttttttct aaaagaataa agtccacttt tacattgata
     1741 tcatcataat tgaataatgc caaagaaact tctgtgtgtt ctgactcacc attactttc
//
LOCUS       DAZPAC010000428         1793 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZPAC010000428 DAZPAC010000000
VERSION     DAZPAC010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.438"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            67..609
                     /locus_tag="ACHUPM_004633"
     CDS             67..609
                     /locus_tag="ACHUPM_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0872181.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            689..1363
                     /locus_tag="ACHUPM_004634"
     CDS             689..1363
                     /locus_tag="ACHUPM_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0872182.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            1379..>1793
                     /locus_tag="ACHUPM_004635"
     CDS             1379..>1793
                     /locus_tag="ACHUPM_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR0872183.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT          571 a          321 c          393 g          508 t
ORIGIN      
        1 agaaatttgt ttccaagaga tacagactct taacaaccaa aaaaaaattt tattaaggaa
       61 catattatga aacgttcaat tattgctgcc gctgtctttt cttctttttt tatgagcgct
      121 ggagtatttg ctgcagacgt tgataccgga acattaacta ttaaggggaa tattgcagaa
      181 tctccgtgta aattcgaagc gggtggtgat tcagtaagta ttaatatgcc gactgtacca
      241 accactgtct ttgaaggtaa agctaaatat tctacctatg atgatgcagt cggtgtaacc
      301 agcagcatgt taaaaattag ctgcccgaaa gaagttgctg gtgtaaaact ctcgttgatt
      361 accaacgata aaataaccgg taacgataag gcgatagcca gtagcaacga taccgtgggt
      421 tactatctct atttaggtga taacagcgat gtcctggatg tttctgcacc ttttaacatt
      481 gagagttata aaacagcgga aggtcaatat gctattccgt ttaaagcaaa atacctgaaa
      541 ctgacagata actcagtgca atcaggtgat gtgttatctt ctctggttat gcgtgtggcg
      601 caggattaat aaataaaatt gtaagtttct ccaggtaata aacatggccg ctatcccatg
      661 gcggcctttt aatttaagag gcattaaaat gaaaggatta ttatctttac tcattttttc
      721 tatggtcctt cctgcacatg ccggaattgt tatctacggg acgcgcatta tttacccggc
      781 agaaaataaa gaagtgatgg tgcagttgat gaaccaggga aaccgttctt cgctgctgca
      841 ggcgtggatt gatgatggcg atacgtcatt accaccagaa aaaattcagg ttcctttcat
      901 gttaacgcca ccagtggcaa aaataggggc aaattccggg cagcaagtaa aaatcaaaat
      961 tatgccgaat aaactgccca ctaataaaga aagcattttt tatctgaatg ttctggatat
     1021 tccaccaaat agtccagagc aagaaggtaa gaatgcactg aagtttgcga tgcaaaacag
     1081 aattaagttg ttttaccgac cagcgggtat tgctccggta aataaagcga catttaaaaa
     1141 attgctggta aatcgcagtg gcaatggttt ggtgataaaa aatgactcag ctaattgggt
     1201 gacgatttcg gatgtcaaag ctaataatgt caaagtcaat tatgaaacta ttatgattgc
     1261 ccccttagaa agtcagagtg ttaatgtcaa aagtaataat gcaaataact ggcatctgac
     1321 cattatcgat gaccatggca actatattag tgacaaaatt taattgcagg agctgcctat
     1381 gttgagaatg accccacttg catcagcaat cgtagcgtta ttgctcggca ttgaagctta
     1441 tgcagctgaa gaaacctttg atacccattt tatgataggt ggaatgaaag accagcaggt
     1501 tgcaaatatt cgtcttgatg ataatcaacc cttaccgggg cagtatgaca tcgatattta
     1561 tgtcaataag caatggcgcg ggaaatatga gattattgtt aaagacaacc cgcaagaaac
     1621 atgtttatca agagaagtta tcaagcggtt aggcattaat agcgataact tcgccagcgg
     1681 taagcaatgt ttaacatttg agcaacttgt tcagggtggg agctatacct gggatatcgg
     1741 ggtttttcgt ctcgatttca gtgtcccgca ggcctgggtg gaagaactgg aaa
//
LOCUS       DAZPAC010000429         1779 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZPAC010000429 DAZPAC010000000
VERSION     DAZPAC010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.439"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            48..1031
                     /locus_tag="ACHUPM_004636"
     CDS             48..1031
                     /locus_tag="ACHUPM_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR0872184.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            1077..>1779
                     /locus_tag="ACHUPM_004637"
     CDS             1077..>1779
                     /locus_tag="ACHUPM_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase"
                     /protein_id="HFR0872185.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKR"
BASE COUNT          592 a          275 c          308 g          604 t
ORIGIN      
        1 taaggatgag tactatcata ttttattagt tcaggcaaga gaacacgatg agcaatgatt
       61 accctttagt atccataata ataccgacgt ataattcatc tgattacatt actgaaactc
      121 taacgaaatt agaaaaacaa acttacccaa attttgaaat tgttattgtt aatgatggtt
      181 ctaaagataa cacatcaaac gttttgagag agtatgggtt aacccactct cgattaatta
      241 ttatcaataa agaaaatggc ggtgtttcgt ctgccaggaa tacaggtatc cgcaaggcgc
      301 aaggacagtt tatatgtttt atggatgatg atgatgagat agatcctaac tatctgctga
      361 agatgtatac cagacaacat gagacgggag gggatgcgat ttattgtggg ctttatggcc
      421 attatataaa agatggtgtt acttactcac ctataaatac agagtttaat gaaggatctt
      481 tacttttcga ctttttttat aaaaaggtta gattccatat agggtgcttg tttataagaa
      541 aacaacttct ggaagagaat aatctttttt ttgatgaaga tttacgacta ggagaagatc
      601 tggattttat ctatcgactg ctaattacat gcgatatgta tgcggttcca tattatatgt
      661 ataagcataa ctatagagaa aattccttaa tgaactcatg tagaaccatc actcattatc
      721 gacatgagtc atttgcgcac gaaagaatct actcttctgt gatgcagtta tacaaaggta
      781 acaggaaaga agaaattcat acattattga gtcaaaatag agcttatcat aaaactcgtt
      841 atttatggaa tgttctactt aatggtgatt ttgaactatt aaatcaatta gttgaaagca
      901 atgaaaaaga attgaatgat tgtaatcttt ctggcaagag agataaaaga cgagcaaaaa
      961 tattagcatc aaaaaattat attctctgga ggatggtaag actggtaaat aggaaaaaga
     1021 ataaacgtta gatcttgcat taaataatcc gtcctcgtag cataggttga aactatatgt
     1081 cattttgttg gaatgaaatt aattctggta taaagtcttt aattctcata ttatgtattt
     1141 tttctttgat gactttgtct ttatgggatg atgttgcaac aaagtttctt catgcagctg
     1201 gaattatatc tgcattgtat tttctaataa caccaaaaaa aacaataact aacaatccta
     1261 ctttgttaat tttcatctca ttatgtcttt tgggaatcat aaatatcatc tggtattcac
     1321 attataaagt ttcaggctct gtttatacca atgcatatcg tggcccaatg gaaactggga
     1381 aaattgcctt gtgtagtgct tttattttct tggttctttt tgctaaaaat gagttgagaa
     1441 caaaaataaa atttgggaaa ctaattctgt tcgcatccct ggcaacgcag ttactttttt
     1501 ttgcgcatgc catgtggcaa catttctatt taaacgttga ccgtgttgca ttatcagctt
     1561 cccacgctac aacagcaggc tacatcatcc ttttcccttc tttactggca tcaattctca
     1621 ttttaaaatc cgactttaga cataaaacaa cattatatac aattaacttc atgcttagct
     1681 tatgtgctgt catagtaact gaaacgcgtg cagccatatt agtgtttcca ttctttgcgt
     1741 tactattaat cgtaatggat agttatatta ataagcgaa
//
LOCUS       DAZPAC010000430         1769 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZPAC010000430 DAZPAC010000000
VERSION     DAZPAC010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.440"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..74
                     /locus_tag="ACHUPM_004638"
     CDS             <1..74
                     /locus_tag="ACHUPM_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0872186.1"
                     /translation="RLKGKLTPALLQILIREIKGSSH"
     gene            74..421
                     /gene="tnpB"
                     /locus_tag="ACHUPM_004639"
     CDS             74..421
                     /gene="tnpB"
                     /locus_tag="ACHUPM_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0872187.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            441..1595
                     /locus_tag="ACHUPM_004640"
                     /pseudo
     CDS             441..1595
                     /locus_tag="ACHUPM_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1599..1769
                     /locus_tag="ACHUPM_004641"
                     /pseudo
     CDS             1599..1769
                     /locus_tag="ACHUPM_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          422 a          475 c          503 g          369 t
ORIGIN      
        1 tcaggcttaa aggtaaactg actccggcgt tattacagat acttatccgc gaaataaaag
       61 ggagcagcca ctgatgatat ctttccctgc aggttcgcgt atctggctgg ttgcaggtat
      121 caccgatatg cgaaatggct ttaacggcct ggcctcaaaa gttcagaacg tcctgaagga
      181 tgacccgttc tccgggcatc tgttcatctt ccgcggacgc cggggtgacc agataaaagt
      241 gttgtgggct gacagtgacg gactgtgcct ctttaccaga cgcctggagc ggggccgctt
      301 cgtctggccg gtcacccgtg acggcaaggt gcaccttact ccggcccagt tatccatgct
      361 tcttgagggc atcgactgga aacacccgaa acgaacggaa cgcgctggca tccgcatata
      421 acccgttgta aagtgaggat atggacacct cacttgctca tgagaatgcc cgcctgcggg
      481 cactgttgca gacgcaacag gacaccatcc gccagatggc gaaatacaac cgcctgctct
      541 cacagcgggt ggcggcttat gcttccgaaa tcaaccggct gaaggcgctg gttgcgaaac
      601 tgcagcgcat gcagttcggt aaaagctcag aaaaacttcg cgcaaaaacc gaacggcaga
      661 tacaggaagc acaggagcga atcagcgcac ttcaggaaga aatggcggaa acgctgggtg
      721 agcaatatga cccggtactg ccatctcccc tgcgccagtc ttcagcccat aaaccgttac
      781 ctgcctcact tccccgtgaa acccgggtca tccgtccgga agaggaatgc tgcccggcct
      841 gtggtggcga actcagttct ctgggatgtg atgtgttaga gcagctggag cttatcagca
      901 gcgcctttaa ggttatcgaa acacaacgtc cgaaactggc ctgttgccgg tgtgaccata
      961 tcgtgcaggc accggtgcct tcaaaaccca ttgcacgcag ttatgccgga gcggggcttc
     1021 tggcccatgt tgtcgctgga aaatatgcag actatctgcc gttataccgc cagtcagaaa
     1081 tataccgtcg tcagggcgtg gagctgagcc gtgccacact ggggcgctgg acaggtgctg
     1141 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
     1201 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
     1261 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa atgcctccgg
     1321 cggtctggtt cgcgtactca ccggaccgga aaggtatcca tccacagaat catctggccg
     1381 gttacagcgg agtgcttcag gccgatgctt acggtagtta ccgggcgttg tacgaatccg
     1441 gcagaataac ggaagcccag cagcgtatcg gtgaactgta tgccatcgag gcagaagtcc
     1501 ggggatgttc agcagaacag cgtctggcgg caagaaaagc cagagccgcg ccactgatgc
     1561 agtcactgta tgactggata cagcaacaga tgaaaattca ttcgctgaaa atggaatgtc
     1621 tccacggaga acactattat ccatcgggaa atagtgcggg caacagtgtt tagttatatc
     1681 gagtgcgatt acaatcgctg gcgacggcac cgttggtgtg gtggtctcag tccggaacaa
     1741 tttgaaaacc agagcctcac ttacgactg
//
LOCUS       DAZPAC010000431         1754 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZPAC010000431 DAZPAC010000000
VERSION     DAZPAC010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.441"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(13..285)
                     /locus_tag="ACHUPM_004642"
     CDS             complement(13..285)
                     /locus_tag="ACHUPM_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872188.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            complement(368..892)
                     /gene="tnpA"
                     /locus_tag="ACHUPM_004643"
                     /pseudo
     CDS             complement(368..892)
                     /gene="tnpA"
                     /locus_tag="ACHUPM_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
     gene            1308..>1754
                     /gene="ospC4"
                     /locus_tag="ACHUPM_004644"
     CDS             1308..>1754
                     /gene="ospC4"
                     /locus_tag="ACHUPM_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HFR0872189.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNILRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNL"
BASE COUNT          512 a          367 c          388 g          487 t
ORIGIN      
        1 cggggctgaa aatcaccacc acttcgggtc atccgccttc atctccgctt ctgtttcgta
       61 taaatcaaaa cgacggcagg tatggcagaa cgtgacgtat tcgtacggtg aatccgggta
      121 cattttgtgg aaggtttccc agaaacagac cgtgcaggag ggatgaccgg cgataaagtc
      181 tgtaaatacg gactgataag ggtgattatt ggctctggcg acggctttca gaacctcttt
      241 caccattctg gtgtggactt tctggtgctc caggttgtgt gacatgggaa ctcattctgg
      301 atggttactc tgaaagctca tattctgcca caccccgatt tgcagccgcc aggctgccgt
      361 ggttcttacg ccagaaggga tttgtaactg gtctggtcgg ctccggcgta tcagtcagtg
      421 ccaccgggac aggcatgcat tcctgtatgt catcatcgct cagtaagccg tcctcgtact
      481 ggcttttcca tttaaacagc aggttatcat tgatatcgtg ttctctggcg atccgggcaa
      541 caacagcacc aggctgtaat gcctgcttag ccagacggac cttaaattca cggctatagc
      601 tggttcgccg ttcttttcgc catgagcctt ctctgatttg aggctctgtt aattccttct
      661 ttctgttggc ataaaggatg gcgtcaagtt gagcgaatga aactgaatcg ggcaatggcc
      721 atgcgatacc ggatgcaaga aatcgctgaa aaagcgtatg tattgtggaa tgactgagac
      781 ccagacgctg agcgatggcc cggatggtca gtttatcttc aaatcttaaa cgcagggcat
      841 caggcaaata agaacggaag cagggaatat cttttgttgt ctgggaattc atcgttcgtg
      901 tccatcaata tagatgggcg cgattgttgc cagacaggac aattttcaca agacgtcgct
      961 gatggggtgc ttacttataa gaggactcta tcatcgccaa tcatcagaca ttgaaataga
     1021 cgacactgag atgacgctta caattctttt ggtttctgta ataagtaatc tgacatcccc
     1081 cgtgcccaga agaggagagg gatatcaagc ccaagggtta ataattgata ctacggttaa
     1141 aattgccttc agtaaaggaa acgtgattaa attcagggga tttcagagtg aaaaccatac
     1201 aacatattaa aatctgttca tttagctata atacatacaa gaaagtgtcc agttagataa
     1261 tgttatctaa ataaccacag ataaaaacgc acataattgg gggggggatg aaaatacctg
     1321 aagcagtaaa tcatattaat gttcagaata atattgatct tgttgatgga aaaacgaatc
     1381 cgaataaagc aacaaaagca ttacaaaaaa atatattacg cgtaacaaac tcatcttctt
     1441 ctggcataag tgaaaaacat ttagatcact gtgctaatac tgtgaaaaac tttttaagga
     1501 agtctatagc agcgcaaagt tatagcaaaa tgttttctca agggaccagt ttcaaatcac
     1561 taaatctttc tattgaagcg ccatctggag cacggtcatc atttcggtca cttgagcatc
     1621 tcgataaggt gtccagacac tatatctctg agataataca aaaagttcat cctctttctt
     1681 cagacgaacg tcacttactt tctattatta ttaattctaa ttttaacttc agacatcaaa
     1741 gtaactctaa tctg
//
LOCUS       DAZPAC010000432         1736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZPAC010000432 DAZPAC010000000
VERSION     DAZPAC010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.442"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            270..539
                     /gene="yacA"
                     /locus_tag="ACHUPM_004645"
     CDS             270..539
                     /gene="yacA"
                     /locus_tag="ACHUPM_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFR0872190.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            539..708
                     /gene="yacB"
                     /locus_tag="ACHUPM_004646"
                     /pseudo
     CDS             539..708
                     /gene="yacB"
                     /locus_tag="ACHUPM_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            791..1381
                     /gene="ospG"
                     /locus_tag="ACHUPM_004647"
     CDS             791..1381
                     /gene="ospG"
                     /locus_tag="ACHUPM_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFR0872191.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          507 a          364 c          408 g          457 t
ORIGIN      
        1 tgactgacga ggtactggcc ctgcgattgt ctgaaaacgg ctcacaactg caccattcat
       61 aatcacatcg cataaaactg tcagcgccag tgataggaca ggtgaaacct gtcaatacgt
      121 accggcttac gccgggcttc ggcggtttta ctcctctgcc atatgcaaca acggagtgcc
      181 gccttacatg ccgctggcgc ggcatatcct ggtgacgata tctgaacagt tatatactgt
      241 gtatatacgt ggtaatgacg aggtggcaaa tggcacaggt taatatgagt gtaagaatcg
      301 acgcagaact gaaggatgct tttatggccg ctgcaaaaag catggaccgt aatggctctc
      361 agttaatccg ggattttatg cgccagaccg ttgaacggca gcacaattct tggttccgtg
      421 accaggttgc ggcaggccgt cagcaactcg aatgcggtga tgtgcttccc catgacatgg
      481 tcgaatcctc tgctgctgca tggcgggatg aaatgagcag gaagattgct gataaatgat
      541 ggagatgttc tggaccatgc tggccatcca ggacaggaag cgcattcgta gtacattgcg
      601 gagcagaacc tgatggccgc cattgaaccg gacgaacgga ttggttattc agccagcagc
      661 ctggcagggc agccgtataa aggccgcaac ggtcgtgtgg aaggaacatc tggaccacac
      721 aaggtagcgt gtaacgttat tttgtgtgaa aatctcttgt gatttttaaa tatcgtaaca
      781 aaggtatgac atgaaaataa catctaccat tattcaaaca ccttttccat ttgagaataa
      841 taattctcat gctggcatag taacggagcc cattctcggt aagttaatag gtcaggggtc
      901 gacagcagaa atctttgaag atgtgaatga ttcatctgct ttgtataaaa agtatgatct
      961 tattggcaac cagtacaatg agattctgga aatggcttgg caagaatctg agctttttaa
     1021 tgctttttat ggcgatgaag catccgttgt tatacagtat ggcggagatg tgtacctccg
     1081 aatgctgcgc gtgcctggga ctccccttag tgacattgat acagctgata tccctgataa
     1141 tatagagagc ctttatctac agttgatatg taaattgaat gagttgagta taatccatta
     1201 cgatcttaat acaggtaata tgctgtatga taaagaaagt gaaagtttat tcccaataga
     1261 ttttcgcaat atttatgctg aatattacgc tgcaaccaaa aaagataaag agattatcga
     1321 ccgacgatta caaatgcgta caaatgattt ttattcgtta ttaaacagga aatatttata
     1381 gccgtatttg ttgatgctat aagccagagg ctcttctatg agaatatggg gaccattccc
     1441 tcgcgatatt aacgtaatat cttaattgta tgcaccgcca gaatgcgttc agtgcgatca
     1501 cggcagcaga caggtaaaaa tagcaacaaa ccacccgaaa aaccgccgcg atcgcgtatg
     1561 ataaatttta accacatgca tacctgtgca gcaaggcgaa tcacgcacgg atttccagcc
     1621 agagacacct gaaacgtaac cggctcattt aaaccgtctg gtttgttacc tccggttcca
     1681 caaaaataat gtccatcatt ttgaagtgga cactatcgta tggaataccg gacctg
//
LOCUS       DAZPAC010000433         1731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZPAC010000433 DAZPAC010000000
VERSION     DAZPAC010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.443"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            71..166
                     /locus_tag="ACHUPM_004648"
     CDS             71..166
                     /locus_tag="ACHUPM_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFR0872192.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            292..1029
                     /gene="erm(B)"
                     /locus_tag="ACHUPM_004649"
     CDS             292..1029
                     /gene="erm(B)"
                     /locus_tag="ACHUPM_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFR0872193.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          642 a          287 c          277 g          525 t
ORIGIN      
        1 aattttgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
       61 gggattcgtc atgttggtat tccaaatgcg ttatcaaatg cgttatgtag ataaaacatc
      121 tactgttttg aaacagacta aaaaaagtga ttacgcagat aaataaatac gttagattaa
      181 ttcctaccag tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc
      241 gtttaacttc tgtatttgtt tatagatgta atcacttcag gagtaaatta catgaacaaa
      301 aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca aataataaaa
      361 caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa agggcattta
      421 acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga cagtcatcta
      481 ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat tcaccaagat
      541 attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa tattccttac
      601 catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc tgacatctat
      661 ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac actagggttg
      721 ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga atgctttcat
      781 cctaaaccaa gagtaaacag tgtcttaata aaacttaccc gccataccac agatgttcca
      841 gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt
      901 caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt aaacaattta
      961 agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt atttaacggg
     1021 aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta ctaaagggaa
     1081 tgtagataaa ttattaggta tactactgac agcttccaag gagctaaaga ggtccctaga
     1141 ctagcaagaa gtacacaaga agccttaaag attatagaaa agcttttcca tgaaatgctt
     1201 aacattattt aatgttaaca tgtggctttg tggtaactac cacgagtggt aacttgtaac
     1261 atgaaaaaag ttacaagtta caagtggttt atttacgaac ataaaggcta ggaaagcact
     1321 tgataagtat atcaagtgct ttttttccgt tcgattttac tatccaagat accgcttgac
     1381 gtacctaata ttttagtgtg ctaagggctt tgctttttct ggttatagtt cgttcaaaaa
     1441 agaccgcaag ggtaaatgca cgatcataga tcgcccttgc agctttttaa tctctccact
     1501 tttttataac caagcaaacc accaagcaca ctgaaaaaat aattaggaac taaagcgaag
     1561 tgcgtatctt ggataaatct tcactcgatt ttaaaagatt aagcacccag ccaaagcgcc
     1621 taaaaaaata atttttttaa agaaaggaag taatttaaat gactacaatt ttaagcgact
     1681 acaataaatc tatcctaatt gaattactaa acaccaatcg ccaaattatc g
//
LOCUS       DAZPAC010000434         1693 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZPAC010000434 DAZPAC010000000
VERSION     DAZPAC010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.445"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..799
                     /locus_tag="ACHUPM_004650"
                     /pseudo
     CDS             1..799
                     /locus_tag="ACHUPM_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972061.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            914..1255
                     /locus_tag="ACHUPM_004651"
                     /pseudo
     CDS             914..1255
                     /locus_tag="ACHUPM_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1213..1693
                     /locus_tag="ACHUPM_004652"
                     /pseudo
     CDS             1213..1693
                     /locus_tag="ACHUPM_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          424 a          409 c          499 g          361 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc agtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggg cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggccagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtcaggc ccggatgttc atcagttaca gcgaagtcag cataaaaaat
     1261 cccgacaaca gcggccaggc gctcccgctc acgtatgtct gttgccggga gcaggcagag
     1321 gacggtgcct gctggcatct gctgacgtca ggaaaagcgg cgagtgccgc cgatgcacga
     1381 cgtattgtca gccattacga gcgacgctgg ctgacagagg aataccacaa ggcgtggaaa
     1441 agtggtggta catggaatcg ctgcgaatgc agacccggga taaccttgag cgcatggtgg
     1501 ttatccaggc gtttatagcg gtacgggtgc tgggcctgcg gcagggaggt gtcagtgaag
     1561 aaacacaaaa tgacagctgt gagaaaatac tgacgccgac agagtggaaa ctactgtggg
     1621 ttaagctgga aggaaagccg cttccggtgc aggcaccgac cctgaaatgg gcctggggcg
     1681 atggcatgac agc
//
LOCUS       DAZPAC010000435         1678 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZPAC010000435 DAZPAC010000000
VERSION     DAZPAC010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.446"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(28..978)
                     /gene="corA"
                     /locus_tag="ACHUPM_004653"
     CDS             complement(28..978)
                     /gene="corA"
                     /locus_tag="ACHUPM_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR0872194.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1130..1186)
                     /gene="ysgD"
                     /locus_tag="ACHUPM_004654"
     CDS             complement(1130..1186)
                     /gene="ysgD"
                     /locus_tag="ACHUPM_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR0872195.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            1348..>1678
                     /locus_tag="ACHUPM_004655"
     CDS             1348..>1678
                     /locus_tag="ACHUPM_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872196.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          437 a          431 c          421 g          389 t
ORIGIN      
        1 aaaaaatagc tttatccggc actcatatta cagccagttc ttgcgcttaa agtacagata
       61 cggtgccagg cccgcgagga tcataaagat aatcgcgcct gggtagccga aactccactt
      121 cagttctggc ataaactcaa agttcatccc atagctggaa gcaacgagcg tcggcggcag
      181 gaataccacg gataccaccg agaagatttt gatgatgcgg ttctgctcga tgttgataaa
      241 acccatcgcc gcctgcatca ggaagttcac cttctggaac agggattcgt tatgcggcag
      301 cagggattcg atatctcgca ggatttcacg cgcctgctcc agttgcccac ccggtaaacg
      361 cgctttacgc accaggaagt tgagcgcgcg ctgggtatcc atcagacaca ggcgaacttt
      421 ccagccgata tcttccagtt ccgccagagt ggagagcgcc tcgtcgtact catcgccctg
      481 atgcccttcc ataatcaccc ggctcaactg ctccaggtcg ctatagatat tttcaatttc
      541 atctgccaac tgttcgattt tggtttcgaa cagatccagc agcagctcgt aggcgttacc
      601 gtctaccatc gactggctac gggcacgcat acgatacaga cgaaaagcgg gcagttcacg
      661 ctcacgcaga gtaaacagac gaccatcacg gatggtaaat gccacagtgg agttaccggc
      721 gtgatcttcc gcatcttcaa agaagaagaa ggagtgaata tgcaggccgt cgtcgtcttc
      781 aaagaaacgt gccgatgctt cgatgtcttc cagttccggg cgggttgcca ggctctggcc
      841 aagttcagat tgtacgcgca gtcgctcgtc gtcgtccggt tcgacaagat caatccatac
      901 tgcatttaca aggggttgtg actcttcgac ttccagccgg gtcagtcggt tattttccag
      961 ttgaaatgcg ctcagcatga ccgggactcc caatgcttaa aatatcggac agttcggtgg
     1021 gcacacagaa acaaattggg tttcagacca ttaaacagcc tgactcagcg cgacgggaaa
     1081 aattgaggtc gctgacaacc gctaaggcta tcagcaaaaa gggatagcct taggagttga
     1141 tcctggatga caggatagtg agccagtatc tactgggtgt gtccaaggcg aatgtcctct
     1201 tagagtgatc gtgcgcgcat gttacgccag caaaattttg ccgtcaacac gcaacagaac
     1261 accacagaac aatagttgaa cttcgcgctt tttggtactc gtcaaggtcg ctaacaggat
     1321 aaaaaaatcc aacatccagg acctataatg gcgcatcggc tactcattgg taaaggaatg
     1381 atcaccttga atctcaaacg gatttttctc gccctcacct tacttccctt atttgctgtt
     1441 gccgctgatg attgcgcact ctcagatccg atgctgaccg tacaggcgta taccgttaat
     1501 cctcaaacag agcgggtgaa aatgtactgg caaaaagcca atggcgaagc gtggggaact
     1561 ttacatgctc tgctggtgga tatgaatagt cagggtcagg tgcagatggc gatgaacggc
     1621 ggcatctatg atgaaagcta tgcgccgctc ggtttgtaca tcgaaaacgg tcagcaga
//
LOCUS       DAZPAC010000436         1676 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZPAC010000436 DAZPAC010000000
VERSION     DAZPAC010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.447"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..889)
                     /locus_tag="ACHUPM_004656"
     CDS             complement(<1..889)
                     /locus_tag="ACHUPM_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0872197.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHP"
     gene            complement(989..1345)
                     /locus_tag="ACHUPM_004657"
     CDS             complement(989..1345)
                     /locus_tag="ACHUPM_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR0872198.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(1481..1588)
                     /gene="rprA"
                     /locus_tag="ACHUPM_004658"
     ncRNA           complement(1481..1588)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHUPM_004658"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          538 a          333 c          392 g          413 t
ORIGIN      
        1 tgggatgcag gcagacggta aacagagcaa taaatgagat aaaggtgtac agcatcagta
       61 atgttgttgg gcgaacggta ttacgaatga gtggagcggt aataaataca caaagcagcg
      121 aacccacggt gtagatactg agtagtttga tcgacatagt gtatgacatg cctgcaacaa
      181 attgtccgta ctgtgccagc cactggctaa ccagataaaa cgttgccatt gagatatagc
      241 catataaggt ataactggct aaatcgataa ttgaacagcg atgttccgta gagctggtgg
      301 ttttctttat gacaggtaag cgacgacccg gatggggtgg gaacgtacaa cggtataaaa
      361 acagagcgtt aataaacata atgcctgcag caatcataaa ggaccaaccg aaccacagtt
      421 cagcccacac taacaggcta atgattagcg gtaataaaaa ttgtccgctg gaaacaaatg
      481 ctttaattaa aatattggct gtcccaggtg agcgtggaaa agcttccatc aaactgggat
      541 aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag aaagccaaaa acataagcga
      601 taatgatgtt attggtctgc aggatgccaa aaaagaaggc catatagcag cacatcccga
      661 gcatgataaa agggcggcga ccaaagcgat cggataataa tcctgcaaaa agcaagacac
      721 ttaatcgacc aatgcccagc gatgagataa ctatcgagac acccgcggca ttagtctgcc
      781 aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat gacgcccata ccatgcacca
      841 ggtaattaaa atacaaccca agtgcggtag ggtaataggg atttttcata ggttctactt
      901 agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt atccattcat tattgttgta
      961 acaacgacaa ttcctttctg tggaaaaatt aacaaagccg ctccagatcg tgtgcatata
     1021 attcagcggc taccgattga tagatcttcc agtcaataaa attgccatca gggtagactt
     1081 gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt gttgccgatg cggttattta
     1141 ttttctctat gatggcatga agatgttttt ttcgttgctg acgcatagcc aacagttgct
     1201 cgactacaca atcaggaatg gcgcatttgc cattttccca ctgacgccag ctttcactgt
     1261 caccggtctg ggcaatccag gtcgcgcatt catcaatagt catggcaaaa atatggcgga
     1321 gtgcctggag ttcataagcg ttcatcgtgt aatggctgca tgaaataaac aataaaatat
     1381 aaataacatt atcagcctgc tgacggcttg aagagagtca cagtatcttg tgcaacatta
     1441 ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg ttaaaaaaaa gcccatcgtg
     1501 ggagatgggc aaagactaca cacagcaatt cgttgtttca ctcaggggat ttccatgctt
     1561 ataaatcaat atgttgattt ataaccgtga gctaatagta ggcatacgga ctttttgcgt
     1621 cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat ttgcgacagg tactac
//
LOCUS       DAZPAC010000437         1667 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZPAC010000437 DAZPAC010000000
VERSION     DAZPAC010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.448"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHUPM_004659"
     CDS             complement(<1..105)
                     /locus_tag="ACHUPM_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872199.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGK"
     gene            complement(183..604)
                     /locus_tag="ACHUPM_004660"
                     /pseudo
     CDS             complement(183..604)
                     /locus_tag="ACHUPM_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
     gene            complement(588..815)
                     /locus_tag="ACHUPM_004661"
     CDS             complement(588..815)
                     /locus_tag="ACHUPM_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR0872200.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYAPKVYDEYRKAKRPWKVIHENQA"
     gene            892..1299
                     /locus_tag="ACHUPM_004662"
     CDS             892..1299
                     /locus_tag="ACHUPM_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0872201.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1503..1658
                     /locus_tag="ACHUPM_004663"
     CDS             1503..1658
                     /locus_tag="ACHUPM_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR0872202.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT          470 a          436 c          344 g          417 t
ORIGIN      
        1 cttccctaat gcggcatctg gatcaatatt cagaatctca gcaagcctga atatttccgg
       61 cttatccggc gtaataacct cgagctttat ccagtttgaa gccatttgtt ttcaccttgt
      121 aacgctcgca gcgttacatt taactgatac cgaacaaaac aatccggcac gattaatttc
      181 aatcaatgca ctacgacaga atcgccgggc gacccaccac cgctgaaatg tgctttccgg
      241 taaacggcct ggacagcatc atcatgcgca tcaattgccg tactcaacgc ttcctgcgcc
      301 gccagtaatg cacggcgttc cagggtatcg aagatacaga gtcggtgacg cagctcacgc
      361 ggaagaattg ccagaaccgc agggatcagt ttctgaattt tttccctttg cgctttcgtt
      421 tcacctttca accaacggtg atagatattc tgctgattgt tccagtcctt gcctggtacc
      481 agagacaatt cgccgccccc ctgccgcaga tattcttcag taattgcgtt agcgacccac
      541 gcctgccctt tttcggctgc cagggcaaca gcactgattc gatgtgctca tgcctgattt
      601 tcatgaatca ccttccaagg tcgttttgcc ttacgatatt cgtcataaac tttgggggcg
      661 tactgaagtt ccccgccaga tgcctcttgc aggcgcatcg cgcgaccttc aggaaccagt
      721 tccccccatg cagcaatgct tgccagccta actcctgcgg cattggcaag ctttgttttg
      781 ctgccaaaaa acgctatagc atcaattttc aacatatcga gctccttaga ttttcctaag
      841 gaaactagat cgtagagaaa cctaagtcaa gaaaaattag aattccctaa tatgaaaaac
      901 gaaaccttcg gtgctcgcct cttacatagg cgtaaaaaat taaaactgtc tcaggccgca
      961 ttaggtaagc tggtcaaagt ggctcacgta acaatttctc aatgggaaag agatgaaaca
     1021 cagccggcgg ggaaaagatt attcgcactg agccaagcgc ttcagtgctc accgacttgg
     1081 cttctttttg gggatgaaga taaacaacca ggcgaaccga tcccggataa tcagccagcc
     1141 attctgacag aagatcaaaa agagttactt caactgttcg acgcactgcc tgagtcagag
     1201 caaaaggccc tgttgtcaga gatgcgtgct cgagttgaaa atttcaacaa actttttgaa
     1261 gaactactca aagctcgcaa aagaagcgca aacaaataat cccctttttt ctccacaccc
     1321 tgtaataaaa agcacaaact ttcaaacact tgtgtttttt acatcaaaaa aacttaggtt
     1381 tttctacaca aaaatcttga ccgcccacct taggttattc taaatttcat tcatcaagac
     1441 accgcacggt gttctcagca aacagttccg ctaccccggc gttaagggga aatggggtca
     1501 acatgaatac tatcgatctt ggcaacaacg aatctctggt gtacggcgtg ttccccaacc
     1561 aggacggcac attcaccgcc atgacgtata ccaaaagcaa aacgtttaaa accgaagctg
     1621 gcgcacgtcg ttggttggga agacattcag gtgagtaaaa tgaacga
//
LOCUS       DAZPAC010000438         1658 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZPAC010000438 DAZPAC010000000
VERSION     DAZPAC010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.449"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(51..494)
                     /locus_tag="ACHUPM_004664"
     CDS             complement(51..494)
                     /locus_tag="ACHUPM_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR0872203.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            651..1580
                     /gene="metA"
                     /locus_tag="ACHUPM_004665"
     CDS             651..1580
                     /gene="metA"
                     /locus_tag="ACHUPM_004665"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR0872204.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          399 a          470 c          369 g          420 t
ORIGIN      
        1 tgcgacgctg gcgcgtcttc tccagcctgg gcaggcacgg caggggcagc ttattcctcc
       61 acatacgcca gattcagcaa cggatacggt ttccccaaat cgtccacctc agagcgtccc
      121 gtaaccttaa aacccacctt cttatagaac ccaaccgcct gctcattttg ctcattaacg
      181 ttggttgtca gttccggtgc catcgagagc gcatgctcca ccagcatccg acccacgccg
      241 cagccgcaca catcaggatc gataaacagc gcatccatat gctgcccact taacaacata
      301 aacccaaccg gctgatcccg ttcattaacc gcgacccaca acggcgcttc cggcaggaag
      361 gaacgaacta gcacctccag ctcggcccga tactctgctg ataaaaaatc gtgtgtggca
      421 tcgacagaac gacaccaaat cgcaacgagt tcctcccctt cctcatgccg tgagcggcga
      481 atactaataa ccattttctc tccttttcgt cattcttata ttctaacgta gccttttcct
      541 tgaaactttc tcaccttcaa catgcaggct cgacattggc aaattttctg gttatcttca
      601 gctatctgga tgtctaaacg tataagcgta tgtagtgagg taatcaggtt atgccgattc
      661 gtgtgccgga cgagctaccc gccgtcaatt tcttgcgtga agaaaacgtc tttgtgatga
      721 caacttctcg tgcgtctggt caggaaattc gtccacttaa ggttctgatc cttaacctga
      781 tgccgaagaa gattgaaact gaaaatcagt ttctgcgcct gctttcaaac tcacctttgc
      841 aggtcgatat tcagctgttg cgcatcgatt cccgtgaatc gcgcaacacg cccgcagagc
      901 atctgaacaa cttctactgt aactttgaag atattcagga acaaaacttt gacggtctga
      961 tagttaccgg cgcgccgctg ggcctggtgg agtttaatga tgtcgcttac tggccgcaga
     1021 tcaaacaggt gctggagtgg tcgaaagatc acgtcacctc gacgctgttt gtctgctggg
     1081 cggtacaggc cgcgctcaat atcctctacg gcattcctaa gcaaactcgc accgacaaac
     1141 tctctggcgt ttacgagcat catattctcc atcctcatgc gcttctgacg cgtggctttg
     1201 atgattcatt cctggcaccg cattcgcgct atgctgactt tccggcagcg ttgattcgtg
     1261 attacaccga tctggaaatt ctggcagaga cggaagaagg ggatgcatat ctgtttgcca
     1321 gtaaagataa gcgcattgcc tttgtgacgg gccatcccga atatgatgcg caaacgctgg
     1381 cgcaggaata tttccgcgat gtggaagccg gactagaccc ggatgtaccg tataactatt
     1441 tcccgcacaa tgatccgcaa aacaaaccgc gcgcgagctg gcgtagtcac ggtaatttgc
     1501 tgtttaccaa ctggctcaac tattacgtct accagatcac gccatacgat ctacggcaca
     1561 tgaatccaac gctggattaa tcttctgtga tagtcgatcg ttaagcgatt cagcacctta
     1621 cctcaggcac cttcgggtgc cttttttatt tccgaaac
//
LOCUS       DAZPAC010000439         1649 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZPAC010000439 DAZPAC010000000
VERSION     DAZPAC010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.450"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..441)
                     /locus_tag="ACHUPM_004666"
     CDS             complement(<1..441)
                     /locus_tag="ACHUPM_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0872205.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVV"
     gene            449..950
                     /gene="tnpA"
                     /locus_tag="ACHUPM_004667"
                     /pseudo
     CDS             449..950
                     /gene="tnpA"
                     /locus_tag="ACHUPM_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(968..1636)
                     /locus_tag="ACHUPM_004668"
                     /pseudo
     CDS             complement(968..1636)
                     /locus_tag="ACHUPM_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          423 a          393 c          374 g          459 t
ORIGIN      
        1 cacaacaccc gtgacctgac ggctattccg gtagcgcatc cagcccagtt tcggcagaaa
       61 aatacggctg ttttcctgat cgagcttaac gccctgcggg tagcggaata catcattctg
      121 tccccgcttt ttgaatcggg gaaaagcagc ccgcttccgg aagaagtttt tgtaagcccg
      181 ctcaaggtct ttcagtgact gttgcaatgg ctgtgaggga gaatctttaa gccattgcgt
      241 ttcagtggca tttttccact caaccagcca ggaagccatt ttcccgtaag ggatgtattt
      301 attaccggcc tcatgattct cattctgacg tgccagagca cgattgaaaa cgaaacgaca
      361 tgcgcctgcg aagcgcctca tttcacactc ctgttgacca cctggtctta actggaattt
      421 aaatgcctgt aatcgtttca caggtcaaat gatactttgg tctgtgaaaa aagaaaccga
      481 tatccggcgt ggaaggcatt gtgttttcct gatgcatgtt cacctggtct ttgtcactag
      541 ataccgacgc cagatttttt gacgcgacag aaaaactacg cacttacttt tcaaatgtat
      601 gtgcttattt tgaagctgaa ctggttgaaa tggatggcga accagatcac gtccatttgt
      661 taattaacta acctcccaaa ctggcgatat ccagtctggt aaacagcctc aaaggcgtat
      721 cgggtaggtt actgcgacga gatcgaccag atattgcagt caggtattac tacaaaggcg
      781 ttttgtggag tcctggctat tttgccagta gctgcggagg tgtgccaata tccgtcatcc
      841 gccaatacat tgaacaacag caaacacctg gtcaggtgga aaaccgcgcc ttatatcccc
      901 gccctgaagg atggggtttt acggcgcacc ggataaaaag cgccatctaa cgatggcgcg
      961 tctggaacta ctttttgtta acgtcaaaca tggtggcatc ggtcgccatg tcatcaataa
     1021 cttgtttaat attttcgaaa gccattggcg tactttcgtt attcaggtct ttaccttcgc
     1081 cctgacgaac gactttgata accggtttat tagtcgctgc atcaatcagc tcaccttcaa
     1141 aatagagtcg agtatccatg gtacggtggc ctgtagccat ttgcgtcccc gcaaccacta
     1201 atgcaacagg aaccacttca tagaattgca gcccttcttt gctggtatct acaccggtaa
     1261 tggccccacg gaaaatcaga ctacgcggcc cagcggtggt aaccagtggt ttacgctgcg
     1321 ctatcgcttc tttcatttcg gtgttggtat agttcaaaat tttatccaga actttctgcc
     1381 ctacctaggt agaaggtttt ggcaccggat aataagtgat tgggttccag acgatgctgt
     1441 catatttgct ttgatcaaaa ctcgggtcta cccaacgtaa aacaggttta cctgttgctg
     1501 aggttgtttc ttttaaatca gagtaattgt ttaaaaaacc agaatattta tctggctggg
     1561 tgattttaga ggcacaacca gacagagcca acaagccgca taaagctgca actttcgcaa
     1621 atgatgtggt acgcatgaat agtttcctg
//
LOCUS       DAZPAC010000440         1618 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZPAC010000440 DAZPAC010000000
VERSION     DAZPAC010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.451"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..202)
                     /locus_tag="ACHUPM_004669"
     CDS             complement(<1..202)
                     /locus_tag="ACHUPM_004669"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0872206.1"
                     /translation="MAEPMAQRPICRQNSHRLHDRLGEPQYTDSLHSQGSASSMWMRH
                     VGGHERFRTGDGQLNTQANRYVL"
     gene            complement(162..1618)
                     /locus_tag="ACHUPM_004670"
                     /pseudo
     CDS             complement(162..1618)
                     /locus_tag="ACHUPM_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
BASE COUNT          364 a          464 c          362 g          428 t
ORIGIN      
        1 gcaatacata gcggttagcc tgagtattta gctggccgtc accggtcctg aaacgttcgt
       61 gccccccgac atgacgcatc cacatactgc ttgccgatcc ctgagaatgc agtgaatctg
      121 tatactgcgg ctcacccaga cggtcgtgta agcgatggct attttgccga catatcggcc
      181 gttgcgccat aggctccgcc atgttcggct aacgtcagct tttgcccacc atggatgttc
      241 agcgctttca gcccatagtt aacgtacaaa ccatcacctg gtgcggtcgt gaggcggaat
      301 ccataatcac cctcggcgac caccgttccg ttttgcgcaa tgtgtaacgt tttgtccgct
      361 tccacctcgt cgccctgtaa atcacgtaac gttaatgagc cacccgaccc aataaccgtt
      421 tgcgccttca ccagttgaac gccgacatgg ctatcatcgt gttccagcaa attgagcgtc
      481 gtcagagggt tattcgctat gggatcattc cacggtttag gcacgtcgat aagcacgtcg
      541 cccgtcccgt ttacctgata gttacggcct ttccaggtgt agtcacccgc gccgacaacc
      601 agcgtatcga cgctgatata tccctctgca agcgtcgcag caggaatatc cgtatcgaaa
      661 atgagcgtac cgccattcat ggccagtcca ccaatggatt gctctcccac gtttaccgat
      721 gtggtatttt cactgtcaga ctgcaacatc gcgtgagtaa gcgcagcggt gttgtcgcgt
      781 tccagagtga aggttctgtc tttcagttgg gcaacaccgg cgaattcagt ccctgttgca
      841 tgggtaaagc caaacatctt gtcgtaggat gacagctgca ctcgcatcaa gccatcgcct
      901 ttgagcgcat tggtcagcgt gtaatctcct gcactgttcg ttgatgcgag aatgtcgagc
      961 gtaccggggc ttgcaatggt gacttcactc gaacccatgc ccgttacccc atctgccgta
     1021 tgagacgcaa tggtcaggag gccgtcattg accagcgttt taccgctata agtattccct
     1081 gcattgaaca ccgtatggcc tgcatcatgg atcacttttc cgtctttatc gtcacctgta
     1141 atcagcatat cgacctggta gccggcatca ctgttattag tatgattaaa gacaaaaaca
     1201 ccttcgccag aaccaaactc tactttcgtc gcgttggtta tatacccggc atctgccgcc
     1261 gcctcaccgt gagctgcacc aatgtttagc gttcctacag caggttcaaa aacacctaag
     1321 taaacttctc caccttcgac attcagcgtg ccgctattcg tcagggttaa cgtacccgta
     1381 ccagatgtcc ctacgtacat attgaatgtt tcaagaagag agttctgccc atccaccctc
     1441 acgtcgccct tacttttgtc attgagagct atctgtgtat cacgcgcttt aactatacct
     1501 ccggtggtaa tattcagttc acccgtgcct aatacaccga cctgtagcaa ttgtgcgtta
     1561 gtagatgatg tctttaaatt ccagagactg tccgttgaaa tattaactat ccccttac
//
LOCUS       DAZPAC010000441         1617 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZPAC010000441 DAZPAC010000000
VERSION     DAZPAC010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.452"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            35..586
                     /locus_tag="ACHUPM_004671"
     CDS             35..586
                     /locus_tag="ACHUPM_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR0872207.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            646..>1617
                     /locus_tag="ACHUPM_004672"
     CDS             646..>1617
                     /locus_tag="ACHUPM_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0872208.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQ"
BASE COUNT          502 a          338 c          318 g          459 t
ORIGIN      
        1 tcattttcta cttatattta tataaaggaa tattatgtct gcttttaaaa agtcacttct
       61 tgttgctggc gtagcaatga ttctttccaa taacgtattt gctgacgaag gtcacggcat
      121 tgttaaattt aaaggtgaag ttatctctgc tccatgttca attaaaccag gtgacgaaga
      181 cttaactgta aatctgggtg aagtagcaga taccgttctg aaaagcgacc agaaatcttt
      241 ggctgagcct ttcactattc acctgcaaga ctgcatgttg agccagggtg gaaccactta
      301 ttcaaaagcg aaggttactt tcaccacagc gaatactatg acgggtcaga ctgacctgct
      361 gaaaaacacc aaagaaactg aaatcggtgg tgccacaggc gttggcgtac gtattctgga
      421 tagccagagc ggcgaagtga ctttaggtac tccggtagta attacattca acaatactaa
      481 cagctatcag gaactgaact tcaaagctcg tatggaatcc ccgggcaaag atgctacccc
      541 gggcaacgtt tacgctcagg ctgactataa gattgcttac gagtaatcac attaggccgg
      601 gttcgcccgg ccacatcatt aacggattaa tgataagtgg aacagatgta taaaaaatta
      661 aaattaacca caataagcga attgattaaa aatatttatt gttcattatc cgttctcatc
      721 attggttgtg cgtcagctta tgccgttgaa ttcaacaaag atttaatcga agccgaagat
      781 cgtgaaaacg ttaacctttc ccaatttgaa actgatggcc aattacccgt aggtaaatac
      841 tcattaaacg ctcttattaa taataaaagg acgccaatcc atctggacct gcaatgggta
      901 ttaattgata accaaactgc agtttgtctg actccagagc aattaacatt attaggcttt
      961 actgatgaga ttattgaaga ggctcagcaa aacctgatcg atggttgtta ccctatcgaa
     1021 aaagaaaaac aaattacaac ttatctcgat aaagggaaaa tgcaattatc catatctgca
     1081 cctcaggcat ggttaaaata caaagatgca aactggacgc ctcctgaact ttgggatcat
     1141 ggtattgctg gggcatttct tgactacaat ttatatgcct ctcattatgc accacatcag
     1201 ggcgataatt cacaaaatat aagttcctat gggcaggctg gggttaatct tggggcctgg
     1261 cgcctgcgta ctgattacca gtacgatcag tcatttaaca atggcaaaag ccaggcgaac
     1321 aacctggatt ttccgcgtat ttatttgttt cgcccaatcc cagcaattaa tgcaaaacta
     1381 actataggtc aatacgatac tgaatcctct attttcgact ctttccattt ttctggcgtt
     1441 tcgttgaaaa gcgatgagaa tatgttgccg cctgatctac gtggttacgc accgcaaatc
     1501 acgggtgttg cacaaacgaa tgcaaaggtc actgtctcac agaacaaccg tattatttat
     1561 caagaaaatg ttcctcctgg cccatttgct attactaatt tattcaatac attacag
//
LOCUS       DAZPAC010000442         1614 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZPAC010000442 DAZPAC010000000
VERSION     DAZPAC010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.453"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..491)
                     /locus_tag="ACHUPM_004673"
     CDS             complement(<1..491)
                     /locus_tag="ACHUPM_004673"
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HFR0872209.1"
                     /translation="MSDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSF
                     TFLGYDFKVRTLKNFKGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARR
                     YNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLALVRKQY
                     PKL"
     gene            complement(506..1012)
                     /locus_tag="ACHUPM_004674"
     CDS             complement(506..1012)
                     /locus_tag="ACHUPM_004674"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFR0872210.1"
                     /translation="MLEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHA
                     DSYGYRPGKSAHDALKQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWV
                     ILYCRRWMEAPMQSCENGELITRTRGTPQGGVISPLLANLFHHDAFDLWMEREYRGYR
                     LRGTLTIL"
     gene            955..1349
                     /locus_tag="ACHUPM_004675"
                     /pseudo
     CDS             955..1349
                     /locus_tag="ACHUPM_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          348 a          454 c          348 g          464 t
ORIGIN      
        1 aatagcttcg gatactgttt ccttaccagc gccagctttc gctgagcctt ccggttcgaa
       61 agtctgtatt tagactgcat ccacttgagc agacgtgact gcattgcact ccacagtcga
      121 tagttgaaat ttctggacca gaactttccg tagtactcga tccagcctct cactatcgca
      181 ttgtagcgcc tcgcaaaatc cagcaaactc tcagctgttg agcgatgtat acgccacttc
      241 ttgattgttt ctgttatttt gcgcattgct gcatttgacg cacccggcat gcattttcgg
      301 taccgttcgc ctttgaaatt cttcagcgta cgcactttga agtcatatcc gagaaaggtg
      361 aaactcgttg cgacgtttct ccttttaaac gtgtcaatgt aggcgatatt cgttttccct
      421 gcattcagga ccagcccgac ttccgagaag cgctccgata acctgttctt cagccttgtt
      481 gcatcactca ttcgtgaaca atgtactaca atatcgtcag cgtacctctc aaacggtacc
      541 cccgatattc tctttccatc cacaaatcaa atgcatcgtg atggaacaag ttagcaagta
      601 gcggactaat gaccccgccc tgcggagtgc ctcgtgttcg ggttattaat tctccatttt
      661 cacaactctg cattggcgct tccatccagc gtcgacagta caggatgacc catttaggca
      721 tcccgtggtg ttccagagct ttgagcacca gatcatgtct cacatggtcg aaaaacgcac
      781 tgatgtccac ttccagtatc cagctataac gccagcaccg gatggcacat tgtttcagag
      841 cgtcatgagc tgatttgcct ggtcggtaac catatgaatc agcatggaaa atcgggtcaa
      901 gcttttcttc catgaaaagt tttatcgctc cctgagcaat tcgatcggat actgttggga
      961 tccccagaat gcgttccttg ccattcgact tcgggatccg tttttccagc actggtggcg
     1021 gaaaccatgt tcccgaacat agccggttcc agatcttgta tagattgcca tcacgttgct
     1081 ggtcgaacat tttcaaagtc tgcccgtcgc atccgggggc tcccttgttg cgacgcacat
     1141 ccagatatga tgcccagacc agtgcttttg gtatttcaaa tgattttcgt tgcatcagtt
     1201 acgtcatccc cttttgggtt gtgtcttctg caatgaatgt gatccccatc ccttcgctcc
     1261 agacccatta cagatctttc atcactacta cgaatgggtc cgcccctgtt tcccgatacg
     1321 tgccggaaac aggttctcac gttccctgag atggtctggc ccgggatcct gctgccttca
     1381 caccggatgc cgcgcagtca gtaagcaggt gtcttctgcg cttatcaggg ggcggctgta
     1441 cggccccatt ttgacatcat ctaagatttc gatgcttcat cggacagttt actttcgttc
     1501 agcttcccga accccacgtg acagtagatc tctgcctttt cccgagtcgc tcaccacctc
     1561 attccttaga atgaagcagc accgggtcgt ttggtcagtg ctcctgtaaa ccct
//
LOCUS       DAZPAC010000443         1604 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZPAC010000443 DAZPAC010000000
VERSION     DAZPAC010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.454"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..213)
                     /locus_tag="ACHUPM_004676"
     CDS             complement(<1..213)
                     /locus_tag="ACHUPM_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0872211.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEYYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTS"
     gene            542..721
                     /locus_tag="ACHUPM_004677"
                     /pseudo
     CDS             542..721
                     /locus_tag="ACHUPM_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(738..1436)
                     /locus_tag="ACHUPM_004678"
                     /pseudo
     CDS             complement(738..1436)
                     /locus_tag="ACHUPM_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1515..1604
                     /locus_tag="ACHUPM_004679"
                     /pseudo
     CDS             1515..1604
                     /locus_tag="ACHUPM_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          456 a          320 c          371 g          457 t
ORIGIN      
        1 agacgtcaaa tttaggtctg ataaatttaa tactgcttct tgattctgaa agcactggga
       61 gagtctgtta aaagcaatgc ctcgctcttc cccgggagaa gagttctttt cccattcagt
      121 caggattctc agataatatt cattactggt tgagtttgag ttaatagagg gggagcaaga
      181 aactgatgag tgtgtattat ttacagagaa cataatgcta ggtcaacctt atatttgtga
      241 gaataaatat ttaaaattgt atatttatac aaatttcact taccatttcc gatattaagt
      301 tttaatagtt tccattttac acataacaga agttagttgt aatttattta ttgaggtttt
      361 ctatggaatt ctttttttat tgtgactgca gtcaataata acactgaatt caacattcca
      421 gtgagcaagg aaatgtaatc cgagtaagta tatttgtttt acatcatatt ttagccaagc
      481 tcatttattc ctattataga atgataaatt aaaattactg taaaaaaacg atacacattt
      541 aatacgaggg aggccggcag aaaaacggct tgcggtaagg caggagaaat gtgtgccgcg
      601 aatgaagtcc ttgtatgact ggataaagct ccagaggaaa actttgtcaa accatgctga
      661 aatggtaaag gcgttcaact atatcctgaa tcactggaat gcactgaatg agttttgccg
      721 ggtagtactt ccaatcatta gaatatgtgc ttttcaatga aggaaccgat gactttttcg
      781 tggatttcca cggaacgcga gaagcagatt gttttacgtg ccagccgctt gatgcggggg
      841 cggagggtca ggttgttacg ttcatacgtt gcgtgaagat tttgccggtc aggtgtttct
      901 cctccggcac ttctctggcg tagctgcccc agtcatcgct ggtcaccatg ccaccatgcc
      961 gatacagaac ggagtcaaca gggctaacag ttcccgacag gtctcatcat tccgtggccc
     1021 gcaagtgtaa gccaacacac cgccagtttt agtgttgtac gcataccagg gccagtgttg
     1081 ttgcgccttg ctgccaacga agcgccactg ctcatcaagt tcacagataa gggcaacatc
     1141 agcgtgagcg actggcgcag atgttatccg ccggggcgag agctttttaa tgtccgtatg
     1201 acggtattga tgccgatttt cagtgttctg gcggtataac agccccctgc gccgttatgt
     1261 gccatttcaa cgatctgctc cttaacgccg ggttttctgg cttcataaga gtaagtgagc
     1321 tggaaaacac gtttacatga gcgacaacgg aaacgctcat gccgggaaca actacggcca
     1381 tgtcgataaa cttcatcgga atgacagcgc ggacaatgaa cagtaacggt agtcatggaa
     1441 aacctcaaat accagtagat taccagaaac ttctatcgat tgaagacatt accttttgcc
     1501 gcaataggtg gggtaacatt ggtgaaaacg cattacgccg ggtggcagtt ggaaaaaaat
     1561 tatctttttt cggctctgat cgtggtggag aaaatgcggc aata
//
LOCUS       DAZPAC010000444         1560 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZPAC010000444 DAZPAC010000000
VERSION     DAZPAC010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.455"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..120
                     /locus_tag="ACHUPM_004680"
     CDS             <1..120
                     /locus_tag="ACHUPM_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0872212.1"
                     /translation="AINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(162..932)
                     /gene="yafV"
                     /locus_tag="ACHUPM_004681"
     CDS             complement(162..932)
                     /gene="yafV"
                     /locus_tag="ACHUPM_004681"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR0872213.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVAWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            1113..1559
                     /gene="ivy"
                     /locus_tag="ACHUPM_004682"
     CDS             1113..1559
                     /gene="ivy"
                     /locus_tag="ACHUPM_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR0872214.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
BASE COUNT          417 a          411 c          380 g          352 t
ORIGIN      
        1 gctattaata ttttgacgaa tgataaggta ttcaaggcag ggttaagacg taagatgcga
       61 aaagcagcta tggacagaaa ctatctggcg tcagtccttg cggagagcgg gctttcgtag
      121 tcttaccccg actctccccc agccttaaac acaaccccca ctcaccgcaa cctaaactca
      181 tccgcatcct gccatgccgg aaacttttct ctatattccc gcaatgccat catcgacagc
      241 tccgcatcaa tgcgcgttgc ctgatgcgcg tcggcagtag cgataatctc gccttgtgga
      301 ttaatcaccc ggctgtcacc gcgataatgg cagccgttgc catcgctgcc gacgcgattg
      361 catcccgcca catacgcctg attctcaatc gcgcgggccg tcagcaatgc ctgccagtgc
      421 agagagcgcg gagcaggcca gttggcaacg tagatggcga ggtcataatc gttgagattg
      481 cgcgaccaca ccggaaaacg taagtcgtag cacaccaacg gcaaaatacg ccagccgcgc
      541 catgccacaa tcactcgcgc attgcccgct ttataatgta gatgctcatc tgccatgcgg
      601 aacagatgac gcttatcata aaaatgtacc gtgccgcccg gctcaaccag caaaaagcgg
      661 ttaaccgaac cagactccgt ttgtaatgca acactgcctg caatcagcgc attgcactgc
      721 tgcgccttag ctgtcatcca gtccactacg tcatcttgtg ctagcgacga agctgccgct
      781 tccatggcaa agccgctggt aaacatcttc ggtagaacga tcacatcgcg cccggtaata
      841 ccttccagtt gacgatcaaa atgacgcagg ttggcagggc catccatcca caccagtggt
      901 tgctgcaaaa gcgtaatctt caaaccaggc acggtgtaca actcctttat gcgaagggtt
      961 ttataacttt aacaccttat caggcagttg ccttagcgca gaataaattg ataacaaatg
     1021 ctgatattgg aaatatctga tttgcaaatt atcgtgttat cgccaggctt tagaaggtta
     1081 ataacatggg caggataagc tcgggaggaa tgatgtttaa gacaataacg acagtcgccg
     1141 ctctggtcat cgccaccagt gcaatggcgc aggatgattt aaccattagc agccttgcaa
     1201 agggcgaaac caccaaagct gcatttaatc agatggtaca agggcataag ctgcctgcct
     1261 gggtgatgaa aggcggtact tatactcccg cacaaaccgt aacgttggga gatgatacgt
     1321 atcaggtgat gagcgcgtgc aaaccgcatg actgtggctc gcaacgtatc gctgtgatgt
     1381 ggtccgagaa atctaatcag atgacggggc tgttctcgac tattgatgag aaaacgtcgc
     1441 aagagaaact cacctggctg aatgtgaacg atgcgctttc gattgatggt aaaacggtgc
     1501 tgttcgcggc gttgaccggc agcctggaaa accatccgga tggctttaat tttaaataat
//
LOCUS       DAZPAC010000445         1548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZPAC010000445 DAZPAC010000000
VERSION     DAZPAC010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.456"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="ACHUPM_004683"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="ACHUPM_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFR0872215.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..>1548
                     /locus_tag="ACHUPM_004684"
     CDS             1253..>1548
                     /locus_tag="ACHUPM_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0872216.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSE"
BASE COUNT          387 a          388 c          437 g          336 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaa
//
LOCUS       DAZPAC010000446         1547 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZPAC010000446 DAZPAC010000000
VERSION     DAZPAC010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.457"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..99)
                     /locus_tag="ACHUPM_004685"
     CDS             complement(<1..99)
                     /locus_tag="ACHUPM_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872217.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLI"
     gene            complement(152..556)
                     /locus_tag="ACHUPM_004686"
     CDS             complement(152..556)
                     /locus_tag="ACHUPM_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFR0872218.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(777..1508)
                     /gene="bluR"
                     /locus_tag="ACHUPM_004687"
     CDS             complement(777..1508)
                     /gene="bluR"
                     /locus_tag="ACHUPM_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFR0872219.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          407 a          336 c          333 g          471 t
ORIGIN      
        1 tatcaatttg atagtattgg cctggtgatt atttatgggt agcaataaaa ggacagtatt
       61 tatcatccat agggatagtc tctgtacttt tattcccatt atgctaatgc cttactgaat
      121 tatgaagcat ttcttaagca tccaacttta gctagattaa tggtttatta ttttctacat
      181 cttcaatata taaaagcgta ttatcaatgg cgtagtaact gcgtttgtta tgattaacat
      241 cagtaaccca acggaaaacg cccgcgcctg ccagtgttga acagtattcc cgaaatgtag
      301 attttccgta aatatgaagc aatgcggcct cttttatttt agcagggttc ttggtcgtac
      361 taacttttaa caggttcctg gttcctctta ataacaaaac cgtgtcatcg tgagtaataa
      421 ttctgatgtt atccgtagcc agataataaa tgtaatgtgc aatacggtga tgttttaatt
      481 ctgaataaaa ccaggagaag ttttgctctt ttctcacttg ctcaaacatc ttttgaaaaa
      541 caacgacctg atccatcagg ataataacct cttgttagtt gtgagactgc gtagtgtgca
      601 cgatcggttt taccacttca atctggtctg tcctttggct gtgatatgta cagagtgtga
      661 tagagggatt acctgaattc ttccggtgag cattttgcaa cggaccagct ccggtacaaa
      721 tgctgttgtg ggttcagatt ataacattct gtctaagggg cgggataaag gtgaaattag
      781 ggggcatgaa agatgacttt ataaccttgc tcaccccaat gttgtaaaag ttcgttttgc
      841 cttctcgttg gtgccatgcc tgtccagaca atcaatgttt gcgtcgggaa cagttccggg
      901 tgcggcgatt caatgggatc agcaagaaca gaaatgtgcc atcctgacaa tgataaacgc
      961 catgcttcca gccacaatcg tgctctctct tcaacattcc acgccatcag aatggcttct
     1021 ttaccgggct tacggcgcat ttcgaaaagt gaggctgctg catattcaat taatgcgccg
     1081 tcaaacatac tgctcataat gcgggaggtg ttgtgatcaa gcacgagacg ctggcgaaca
     1141 ggaaggtaaa catgattaat caattgatct actgggtact ctcgacccag tgaaataatt
     1201 ctcgcgcgta gtttggcagg attagccata cgaagaattg acatcatctc ttcttgcagg
     1261 cggttccagt catcttccgt atcctggctg gtggtttcca gtaatgcttt aactttgcct
     1321 acagggacgc cattacttat ccaacgcttg atctcttcga tgcgttgtat gtcttcttca
     1381 tcaaagagtc ggtgtccgcc ttcactgcgc tgtggtttta acaaaccgta gcggcgttgc
     1441 caggcccgga gagtgacagg attaatcccg caacgttcag caacatcacc aatgctgtaa
     1501 taagccacaa ttcctccttg cggtcataaa tctccgccgc ctgtaca
//
LOCUS       DAZPAC010000447         1546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZPAC010000447 DAZPAC010000000
VERSION     DAZPAC010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.458"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            146..1189
                     /locus_tag="ACHUPM_004688"
     CDS             146..1189
                     /locus_tag="ACHUPM_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0872220.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT          435 a          348 c          334 g          429 t
ORIGIN      
        1 cataaagata aaattattgt tctcaaggaa agcatgaagg ggcttcgtaa gcaatattca
       61 ttcggtgcca agacaggact tgatacacag aatctcaact tttatgctct cagactatat
      121 caccacatgt tttacaggct cagaaatgaa cgatagatta tgctttgaag tgcacgataa
      181 caagggttat ttcgtatttc cggatacctg gtttggccca ttgctggggg agtttgaaga
      241 agtgcttgat gcatacgacg ctgatgaaat cagcgagacg agttacatca acaaactccg
      301 acgccttgct caacgggagc cggactttat tgatatacac gctcatctgg catatgcatt
      361 tctggaacag aatgccccgc gtaaggcact gaacgcctct cttaaaggac tggcggcagg
      421 taatcgtatc atcccggaaa gtttctgtgg tgaaataatc tggatgcatc ccgaaaaccg
      481 gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac agcgccatca
      541 ggatgcggtt atgctcactg ataaaatact ggcatacaat cctgaagata atcagggggc
      601 tcgctggttg ttgggttcag agctgttacg caccggcgat catgagcgag ccttcagtgt
      661 actgaaagag catgctgatg aattttcacc ttactggtat gaactcggtc tcctgcattt
      721 tctgaacggg gaacatgtta aggccgccac agcttttcgc catggctttg ccacaaacac
      781 ttacatcgca gaaatgctgt gcggaaatct gcatccattt ccacttgctg tgtggcatga
      841 cttctccggc tctctggata cggcagaaga ttactatgca acatactcac cattatgggg
      901 acaatatccg gaagcattgc tgtttgtaaa ctggctctat aaccattcct ccgtgctgca
      961 tgaacgatca gaaatcatta aatgcgccga aatgctgatt caggaagatg atttcgaaat
     1021 atgcgaaagt attttaagac agcaggagca cctgtggaaa agaattgaca agacactttc
     1081 tgaggaaatt gtacagaaat gcagaaatat gaacggtgaa tatatctggc cctggatatt
     1141 gcccttttcc gcagcaggga tgaaacattc aagcatccag tatcagtaga tattgcatta
     1201 gtgtaacttt aaaacaagta ataatttatt atccaataat aacactgtgt tccttcccca
     1261 ggtgattacc ctggggttat tttctgaaag tttcctttgc aaaaggatag cgttgtaaat
     1321 tactttaacg aattactcca tgaactcatt aaagttcttt cgtaatactt tatcgtcaaa
     1381 cacgttatag ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa tcccctcctc
     1441 tggcgttttc ggttgaactg gtatccccgt ggatttgccc ctatatttcc agacatctgt
     1501 tatcacttaa cccattacaa gcccgctgcc gcagatattc ccgtgg
//
LOCUS       DAZPAC010000448         1536 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZPAC010000448 DAZPAC010000000
VERSION     DAZPAC010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.459"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..1012)
                     /locus_tag="ACHUPM_004689"
     CDS             complement(<1..1012)
                     /locus_tag="ACHUPM_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFR0872221.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGK"
     gene            1267..1513
                     /locus_tag="ACHUPM_004690"
                     /pseudo
     CDS             1267..1513
                     /locus_tag="ACHUPM_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
BASE COUNT          438 a          353 c          275 g          470 t
ORIGIN      
        1 tttttccccc aaaataaaca gacattggtg catcattata agctaatgct ttacccattt
       61 tacccgcagc taaatcaact gatgtggcag aaccattatc ttcaacaaat gcagttacac
      121 gaccaccaaa gtttgctaac tgaacatcct taccacccac ctgaactttt tcataacctg
      181 tagagttttg tttcaataat gtaaaaccgt tcgtgttggt attattatct tgaccaacaa
      241 aataagtctg cccttccacc tgtacttgat aaacttttgt tcctgcagta ccggtcgcat
      301 cagttgtatc ctttgtatct tgtacattac cggtaccaaa ataagtttca acatcagcat
      361 ttttataatt gctaccgaat gctttagcac ctcccacact accatcagtt gcatttacct
      421 tagttaagtc aggagcggct tcgctcttaa gtgcaaccgc agaggacatt ggaacttttc
      481 ctagtgctac gctaaagtta tcaagcccca aggttttagc atcaatttta tcaagggcaa
      541 tctcaatggt ttcaccatcg ttcgcaccta cttgaatttt catagtctga tcagaagcca
      601 gaacttttac accattaaat tgtgtctggt tagaaacacg atcaatttca ctaaggcgtt
      661 gtgtaatttc atcctgaatt gaattcaggt cggagtcaga gtttgtacca ttagtggcct
      721 gtactgacaa ttcacgaata cgctgtaagt tattgttgat ttcagaaagc gcaccttcag
      781 tggtctgcgc aacagaaata ccatcattcg cgttacggga agcctgagtc agacctttaa
      841 tatttgctgt aaaacggtta gcaatcgcct gacctgctgc gtcatcttta gcactgttaa
      901 tacgcaggcc agaagagaga cgttcaatgg cggagctcag agaagactga gatttgttca
      961 ggttattctg ggtcaacagc gacaggctgt ttgtattaat gacttgtgcc atgatctttt
     1021 ccttatcaat tacaacttga tgttattggg ctgttgccca cggtttctca ccgtacccct
     1081 tgtatcggca cctgaatttc gaactttaga aaattttagt ttctctcctg ttttttctta
     1141 acggctgaaa tatccgtaat atacaactct actctgcgca tcatcttcgt gaatcaacta
     1201 ttaaactttg cgcacaaatt gccgataacc cgcttatcta ctgttttcaa tcaaaaggaa
     1261 ttaggtatgg cttcatttac atctcttggc gttggttcga atctgccgct ggatacattg
     1321 ctaaacaacc tgaccatcgc tgaaaaaaaa cgcttaaacc caattactca gcaacagagt
     1381 gacaataccg cacgtctgac agcctatggc acattaaaaa tgtgcattag aaaaattcca
     1441 gactgcaaat actgcgttaa ataaagccga tctgttcaaa agcactaccg ttaccagtag
     1501 caatgaagac ctttgaggta gcctgagttt aacgga
//
LOCUS       DAZPAC010000449         1494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZPAC010000449 DAZPAC010000000
VERSION     DAZPAC010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.460"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(19..378)
                     /gene="traA"
                     /locus_tag="ACHUPM_004691"
     CDS             complement(19..378)
                     /gene="traA"
                     /locus_tag="ACHUPM_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFR0872222.1"
                     /translation="MNTVLSVQGASAPVENKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(412..639)
                     /gene="traY"
                     /locus_tag="ACHUPM_004692"
     CDS             complement(412..639)
                     /gene="traY"
                     /locus_tag="ACHUPM_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFR0872223.1"
                     /translation="MIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVIEENESTFKEL"
     gene            complement(759..1406)
                     /locus_tag="ACHUPM_004693"
     CDS             complement(759..1406)
                     /locus_tag="ACHUPM_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TraJ family protein"
                     /protein_id="HFR0872224.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFGCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
BASE COUNT          498 a          343 c          233 g          420 t
ORIGIN      
        1 acatcgtttt atttcctgtc agaggccaac gacgctcata cccacagcaa taaatacaga
       61 gatgatggca aagccgacca ggaacttgac gtttttggtc atcatgtaca tgaccgcacc
      121 gaccagcacc tcagccagaa caacccattt aacgatactg gagtccttac cgaaggtcgc
      181 cttaaccgta tcgttacctt tagccatcag gtcgccttta ccagcagcca tagctaactc
      241 agggaacaac atcatcagaa cagcaaccgg gatcactgca cgaaccaggc gaagcatatt
      301 cagacgagtg aatttagaaa aaaacgattt attttcaacg ggcgcagaag caccctgaac
      361 acttaaaaca gtattcatat gattcctctg attaatattg acagatgaaa actaaagttc
      421 tttaaatgtt gactcgttct cttcgatcac ctccctgaat atttcttcat tataaaaatc
      481 tggataacgt tgcaaatgat cctgcaacct caccaatgct tcgtttgttt tcgttcttcc
      541 actacgctct ctggctccaa gtaataattt attcgtttcc tcatcaaggg taagtaaaac
      601 ttttctactt ggcgcagatc ttaaagctcc tttaatcacc ctccctccca taaataaaag
      661 agatttagtc aaaatattca cataagacgc acacaaaaat aaacaacaaa atagtaaatt
      721 acgttaacaa aaaccggcac ttttagtgcc gggaatgctt acatttcttt acagtgaagg
      781 ctgttaatat tactagcgaa gtattgatat agataaaata acacatcaga gattatagca
      841 tatcgcaacc actcatccct gtctgagaaa ccatagtgtt ttttaaaatt cataaatatt
      901 tttttagatt ggtcaacaga gatatccatt ctctgagaag aatactcatg actataaccc
      961 aaaagatacg gtattatctt attaaagcga tatttgtcag gttcacaacc aaactccttg
     1021 ccctttataa aaaagccact aactacgtat ctttcaaaga atctccagac aaacaaattc
     1081 aagttattca cttccgtact ttcaaccacc acatcccatg atacatcatt caatattaca
     1141 ccattaataa tactcactcc attcgaggac gagaaagcat caacttcagc agtatgaaat
     1201 aaaatttgcg tatcaatatc aataccagaa aaccatattt cagaatcact attctgacac
     1261 tttattaagt gtaaaaaaac agagtttgag tctatgaatt ttcctgactc tgtgcggaca
     1321 cagactggat attttgcgga taaaataatt tccggcaggc ctgattgacg tatgagtgct
     1381 ctttctctag tatccatcgc acacatagga acctcctcaa aggattctat ggacagtcga
     1441 tgcagggagg gacaagctcc ctgcatcggc gattttcaag ttcaaatttt aacg
//
LOCUS       DAZPAC010000450         1483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZPAC010000450 DAZPAC010000000
VERSION     DAZPAC010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.461"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            56..118
                     /gene="yoeI"
                     /locus_tag="ACHUPM_004694"
     CDS             56..118
                     /gene="yoeI"
                     /locus_tag="ACHUPM_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR0872225.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            108..1466
                     /gene="plaP"
                     /locus_tag="ACHUPM_004695"
     CDS             108..1466
                     /gene="plaP"
                     /locus_tag="ACHUPM_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR0872226.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAQAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
BASE COUNT          295 a          364 c          394 g          430 t
ORIGIN      
        1 ccggatagat atttactaaa aatcgacagt tgttgtcgct gaggaatcca aaaaaatggg
       61 gcaatttttt gcttacgcga cggttatcac cgtaaaggag aatgaccatg tcgcataacg
      121 ttactccaaa cacctctcgc gtggaattgc gtaaaacgct tacgttagtt ccggttgtaa
      181 tgatgggtct tgcctatatg cagccgatga cgctgtttga tacttttggt atcgtttcag
      241 gcctcacgga tggtcatgtg ccgacagcct atgcgttcgc attgattgcg atcctgttta
      301 cggctctgag ctacgggaag ctggttcgcc gctatccttc tgctggctct gcatatactt
      361 acgcccagaa atccattagc ccgactgtcg gctttatggt gggttggtct tctctgctcg
      421 actatctgtt cgcgccgatg atcaacattc tgctggcgaa aatctatttt gaagctctgg
      481 tgccttccat cccatcgtgg atgtttgtgg tggcgctggt ggcctttatg accgccttta
      541 acctgcgtag tctgaaatcc gtagcgaact tcaacactgt aatcgtcgtg ttgcaggtag
      601 tgctgatcgc ggtgattctg ggcatggttg tttatggcgt atttgaaggt gaaggcgctg
      661 gtacgctggc gagcactcgt ccgttctggt ctggcgatgc gcatgttatt ccgatgatta
      721 ccggggcgac aatcctgtgc ttttccttta ccggctttga cggcatcagc aacctgtcgg
      781 aagaaaccaa agatgcagag cgcgtgatcc cgcgtgcgat tttcctgacc gcgctgattg
      841 gcggcatgat cttcatcttt gcaacttact tcctgcagct gtacttcccg gatatctctc
      901 gctttaaaga tccggatgcg tcacagcctg aaatcatgct gtacgttgcg ggtaaagcgt
      961 tccaggttgg cgcgctgatc ttctccacca ttaccgtact ggcgtccggt atggcggcgc
     1021 aagcaggcgt agcgcgtctg atgtacgtaa tgggtcgtga cggcgtattc ccgaaaagct
     1081 tcttcggtta tgtacacccg aaatggcgta ctccggcgat gaacatcatc ctggttggcg
     1141 cgattgccct gctggcaatc aacttcgacc tggtaatggc tacagcgctg attaactttg
     1201 gtgcgttggt ggcgttcacc ttcgttaacc tgtcggtcat ctcgcagttc tggatccgtg
     1261 agaagcgtaa taagacgctg aaagatcact tccagtatct gttcctgccg atgtgtggtg
     1321 cgctgaccgt tggtgcgctg tgggttaacc tggaagaaag ctcaatggtt ctgggtctga
     1381 tttgggctgc tatcggtctg atttacctgg cttgcgtcac caagagcttc cgtaacccag
     1441 ttccgcagta cgaagacgtt gcataatcat cttttctaat aat
//
LOCUS       DAZPAC010000451         1467 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZPAC010000451 DAZPAC010000000
VERSION     DAZPAC010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.462"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            9..452
                     /gene="cmtB"
                     /locus_tag="ACHUPM_004696"
     CDS             9..452
                     /gene="cmtB"
                     /locus_tag="ACHUPM_004696"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFR0872227.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            480..>1467
                     /gene="cmtA"
                     /locus_tag="ACHUPM_004697"
     CDS             480..>1467
                     /gene="cmtA"
                     /locus_tag="ACHUPM_004697"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFR0872228.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
BASE COUNT          334 a          326 c          379 g          428 t
ORIGIN      
        1 gataatttat gcggcttagt gactattttc cagaatcatc aatctcagtt atacattcag
       61 caaaagattg gcaggaagct atcgatttct cgatggtatc attgctggat aaaaactata
      121 tcagcgagaa ttacattcag gcaattaaag attccaccat taacaatggc ccttattata
      181 ttctcgcacc aggcgtggca atgcctcatg cgcgaccgga atgtggggcg cttaaaaccg
      241 ggatgtcttt gacattactt gaacaaggtg tttattttcc ggggaatgac gaaccaataa
      301 aattactcat cggcctttct gctgccgatg ccgattcgca cattggcgct attcaggcgt
      361 taagtgaatt actgtgcgaa gaagaaatac tcgaacaact cttaacagca gcatcagaaa
      421 aacaattagc ggacataatc agccgcggat aattactctc tcctctacag gataatatta
      481 tggaaaacaa gtctgctcgt gcaaaggtcc aggcttttgg gggctttttg acggcaatgg
      541 tcatccccaa tattggtgct tttattgcct ggggttttat tactgcgtta tttattccca
      601 ccggttggct gcctaacgaa catttcgcca aaattgtcgg cccgatgatt acctatttat
      661 tgcccgtgat gattggttct accggtggtc atctggtcgg cggtaaacgc ggcgcggtca
      721 tgggcggaat aggtactatt ggtgtgatcg tcggcgcaga gatcccgatg ttccttggct
      781 caatgattat ggggccgctc ggtgggctgg tcataaaata tgttgataag gcactggaaa
      841 aacgcatacc tgccggtttt gagatggtta tcaataactt ctcattaggt atcgcgggga
      901 tgctcctttg tctgctgggt tttgaagtta tcggcccggc ggtgttaatt gccaatactc
      961 tcgtcaaaga gtgtattgag gcgctggtac atgcgggtta tctgcctctg ctgtcagtca
     1021 tcaatgaacc ggcgaaagtg cttttcctca ataatgcgat cgatcagggc gtctattatc
     1081 cgctgggaat gcaacaggct tcggttaacg gtaaatccat cttctttatg gtggcgtcca
     1141 atcctgggcc tggcttaggg ctgctgctgg cgtttacctt gtttggtaaa gggatgagta
     1201 aacgttctgc gcccggggcg atgattattc acttcctcgg tgggatccac gaactgtatt
     1261 tcccgtatgt gctgatgaag ccgctgacca ttattgccat gattgcgggc ggtatgtctg
     1321 gcacctgggt gtttaactta ctggacggtg gtctggtggc tggcccaagt ccggggtcta
     1381 tctttgctta cctggcactg acgccgaaag gctcgttcct ggcgacaatt gccggtgtta
     1441 cggtagctac cctggtgtcc tttgcta
//
LOCUS       DAZPAC010000452         1459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZPAC010000452 DAZPAC010000000
VERSION     DAZPAC010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.463"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHUPM_004698"
     CDS             complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHUPM_004698"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR0872229.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHAAIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1092..>1459)
                     /gene="cadB"
                     /locus_tag="ACHUPM_004699"
     CDS             complement(1092..>1459)
                     /gene="cadB"
                     /locus_tag="ACHUPM_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="antiporter protein responsible for lysine import
                     and cadaverine export; member of the lysine-dependent acid
                     resistance system 4 (AR4); inner membrane protein; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR0872230.1"
                     /translation="LLAAVKMTALIILITLMNSAGGKASDLFGELTGIAVLLTMLPYF
                     YSCVDLIRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILMFYAR
                     KMHERQSHSMDNHTASNAH"
BASE COUNT          406 a          320 c          365 g          368 t
ORIGIN      
        1 tggtgtaagg cacccacgcg gagtcaaagt ggatggattt cacatccagt gttttcttga
       61 tgaagtcggt gttgtacagc agaccatcat aggtagagtt ggtaattaca gcatgtaccg
      121 gccaggttgc gtttggtgtt tctttcacgc gcttagcaat ggcagcgtgc tggaattcac
      181 tctgtgggat accaccaaga ataccgtaag cgttacgggt cgggcggaaa tagattggcg
      241 taacatcgct catcatcatc aggtgggtca gcgatttgtg gcagttacgg tcaatcagaa
      301 tggtgctgcc tgccggagca gagtacatgc caacaatttt gttcgcagtg gaagtaccgt
      361 tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc gatatactgt tctgcttctt
      421 tgtgtggacc actatgatcc agcagagaac ccagttcaga tactgaaatg gaaatatcag
      481 atttcatggt attcggacca aagaaatcat agaacaggct acctaccggg cttttctgga
      541 atgcagtacc gcccatgtga ccaggagtac agaaagtata tttaccttca cgaacatatt
      601 taaacagtgc tttagtcagc ggaggcagaa tagtgttgat atattcgtca gtggtctgct
      661 tgatcttatt agcaatatct tcagcagcac ccagcgcata ttcaaagaag ctaatctgta
      721 aacgcaggtc attcaggctt acatcgagag tggaatacgt attagcgaac gcgtacaacg
      781 gcaggttctc gttcattttg ctaatttctt cgcacagctc gagattatat ttatcccagt
      841 caaaaataac gccgcacaga cgcgcattgt tttcgatcag ttttaataag tcgtcacggt
      901 cgttcgggta aacaatctgg aagttcagac gttcaagcgc gcgatgaagt tcacggatgg
      961 gttcttcttt aaaataaacc cccatgtgat tcaatattgc aataacgttc atagtcatat
     1021 ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa tgaaaggagg agcctcggaa
     1081 aatactttta attaatgtgc gttagacgcg gtgtggttat ccattgagtg gctctggcgc
     1141 tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc tgacgatgaa ggtacctgcc
     1201 agctcgaagg agcttgcgcc catcagcgcg atgaagcaga acacacaacc cagtacagag
     1261 cagatcaggc tgacaaagtt gcggatgtta acgccttcaa aacgaatcag gtcaacgcaa
     1321 gagtagaagt acggcagcat agtcagcagt actgcgatac cggtcagttc accgaacagg
     1381 tcagatgctt taccaccggc agagttcatc agagtgatga ggattatcag ggcagtcatt
     1441 ttcactgcag ccagcagca
//
LOCUS       DAZPAC010000453         1458 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZPAC010000453 DAZPAC010000000
VERSION     DAZPAC010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.464"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            32..694
                     /locus_tag="ACHUPM_004700"
     CDS             32..694
                     /locus_tag="ACHUPM_004700"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR0872231.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
     gene            1058..1458
                     /locus_tag="ACHUPM_004701"
                     /pseudo
     CDS             1058..1458
                     /locus_tag="ACHUPM_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170527.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          452 a          292 c          290 g          424 t
ORIGIN      
        1 agagtgattg caataaaaat ttttatttct tatgtcaatt gagcatattt taatcgggaa
       61 gcatcatggg ggttctgatt actggagaac tccatttttg ttatttaaga acttacaccg
      121 tgaatttatt ttcagtctag atggtgctgc aactgagcat gacacgctgt taccacgatt
      181 caccgatgat ataagccgtc aatcatgggt tagtgaaaag gtcttctgta acccgccgta
      241 cagcgatatt ccgtcgttcc tcttaaaagc gtctgaagcg gatttagtag ctttcctgat
      301 accgcataga gccaacacca gctactggct taggcacatt tacagcaata accactgtca
      361 tgaaatccgt attcttcatc gtgcagttaa ataccttcca ccagcagggc ataacaggct
      421 tactattagg tcaccgtttc caagtgcagt cgttgttttt aaaaaagaac cacgtaagca
      481 tgaaattaca cagatggttt gctgtgctga tacattgcta ccgttaacca tcattaaccg
      541 tggtggacta agaggaagac ccaccattta cccacctgag acgctcgaca gcttcataaa
      601 actatacagg caaggaaaac ccattaagta cattgcagat gctctcagaa tgcctctgag
      661 cacgtcatac aggattgctc aaaggttgag ctaaaattct cataaatatg gtcatttaca
      721 agacaggtga ttcagatgtt aagaagtatc cctgtgaaca ataaatgacc aatcagtaat
      781 cattctgtga aataattgtt gcaatttata atcgcatctg ttatagtttc tatatgggct
      841 gataaaaaag ttcatgtctt gaagtggtaa cacttgggga ggtgtcagat ggttggcgta
      901 tattaacctc tcattcaggg tatacctcag cgttttaatc atcgtactgt ttcaggcatg
      961 gacttcttta tcagtccatc aatcccccag atattttaat cacacaggct aatcggtttt
     1021 atccggttgg ccttttcacg tatgaggtaa tttatgaatg tctaaatata acccaaatca
     1081 gttttataaa tttgatgtgc gaatctctca actttcagaa gaaattaacg cagcagcaag
     1141 attgatttat atggtcatgg tccatagctg tgacttctat gaagagaaat acaaatcata
     1201 ttcaccaagt attgaccatc tcgcttatat ggctggtgta tcaaagagaa cagcaatcag
     1261 agccattgag acacttgaga aacacggttt aattcaggtg caaaagaatc aggggcgtca
     1321 aagcacttat cgtgtattca cactggatga taagccggaa ttacttgtaa gacctacatc
     1381 acaggaagtt gcagcctcag taagacaaaa tgacaccact accagtgaca aaatgtcatg
     1441 ggggtagtga caaaatgt
//
LOCUS       DAZPAC010000454         1431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZPAC010000454 DAZPAC010000000
VERSION     DAZPAC010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.465"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..439
                     /gene="lpfB-O113"
                     /locus_tag="ACHUPM_004702"
     CDS             <1..439
                     /gene="lpfB-O113"
                     /locus_tag="ACHUPM_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR0872232.1"
                     /translation="SQEKILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLE
                     IAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDV
                     PLNMIRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            464..>1431
                     /gene="lpfC-O113"
                     /locus_tag="ACHUPM_004703"
     CDS             464..>1431
                     /gene="lpfC-O113"
                     /locus_tag="ACHUPM_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR0872233.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDL"
BASE COUNT          386 a          331 c          341 g          373 t
ORIGIN      
        1 ttcgcaagaa aaaatattac gcattattca caccaaaggt gtgtcattgc ctgatgatcg
       61 cgagtcagtc ttctggctga atattaaaaa tattccgcca tcagcgagca acaaagcgac
      121 gaactcgctg gaaatagcag taaaaacgcg tatcaagctg ttctggcgac cagcaaatat
      181 ccgccttatt cccgaagatg ctgcgccgaa agtgaaatgg cgccgggaag ggcgaaatct
      241 gatagcagag aatcccaacc caattcatat cagtgtgatg gatgtcatcg ttgatggcca
      301 tgatgtgccc ctcaatatga tccgtccttt tgaaacgctc acgttacctt tacctgctaa
      361 ttctgcggga ggccaaatga cctggcgatt tattaatgat tacggcgcag tcagtgaccc
      421 gataaaaatg acgctgtaag caagggataa ggataggcgt attatgatga cgaccagaat
      481 agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga tggccgaggt
      541 cagtgtattc aatccggcgc ttctggaaat cgaccatcaa tccggagtcg atattcgcca
      601 gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt ttatcaacgg
      661 taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg ctgatctgca
      721 cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag ttgatgcgct
      781 caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac gtattgaagg
      841 ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc cccaaatcta
      901 catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg ggattaacgc
      961 gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt atggttcaca
     1021 agagacagat accagttatc tcaatctgcg caatggactg aatattggac cgtggcggct
     1081 acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact ccattagtac
     1141 ctggatacag cgcgatattg ccgcgttaag aagccagatt atgattggtg atacgtggac
     1201 ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata ctgataacga
     1261 tatgctaccc gccagccaga atggctttgc tcctgtggtt cgtgggattg caaagtccaa
     1321 cgccaccgtc atcattcggc agaatggcta cgtgatttat cagtctgccg ttccacaagg
     1381 tgcttttgag atcaccgatc tcaacaccgc cagtacaggt ggcgatttgg a
//
LOCUS       DAZPAC010000455         1428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZPAC010000455 DAZPAC010000000
VERSION     DAZPAC010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.466"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHUPM_004704"
     CDS             complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHUPM_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR0872234.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHUPM_004705"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHUPM_004705"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR0872235.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1428)
                     /locus_tag="ACHUPM_004706"
     CDS             complement(822..>1428)
                     /locus_tag="ACHUPM_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR0872236.1"
                     /translation="KGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFC
                     FGKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGAD
                     EEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVP
                     GAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          324 a          397 c          396 g          311 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgccttta
//
LOCUS       DAZPAC010000456         1406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZPAC010000456 DAZPAC010000000
VERSION     DAZPAC010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.467"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHUPM_004707"
     CDS             <1..184
                     /locus_tag="ACHUPM_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872237.1"
                     /translation="TILIYQIITSSSILFIVWGITSIIIRTKNYFSKKKTDLNISFFI
                     AFPISITILLFCRGIL"
     gene            complement(693..1028)
                     /locus_tag="ACHUPM_004708"
     CDS             complement(693..1028)
                     /locus_tag="ACHUPM_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFR0872238.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            1157..>1406
                     /locus_tag="ACHUPM_004709"
     CDS             1157..>1406
                     /locus_tag="ACHUPM_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFR0872239.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAII"
BASE COUNT          474 a          281 c          227 g          424 t
ORIGIN      
        1 aacaattttg atatatcaaa taattacaag cagctccatt ctttttattg tttgggggat
       61 tacatctata ataattagaa caaagaatta tttttctaag aagaaaacag accttaatat
      121 aagctttttt atagccttcc ctatttctat aacaatcctt ttattttgcc gaggtatatt
      181 ataaatataa cataatacat ctatatgggg tgtacatatc cccacataaa aacacatact
      241 ctcattaaca cgggaagaaa ctatgttttc caattctcac tacatgtaca taatcgttct
      301 tgtactatgg tttagtattc tcaagtactt acagagaaag aatagcatat tacatccttt
      361 attttcacta atcctgacac cactaaccat tttaatcatt agacttatga tagatttaac
      421 atacaaccaa taaattataa acaaaataaa aatagggaac attatatgct ttataaaatt
      481 ttcacattaa acacaaagat aaaaacaact atcattacaa tatgtgcatt atcatataat
      541 cttacagcgc aagcttcccc gtcatacaga cctcgtgatc gagttaataa gactatgata
      601 gaactacaaa ctaacggagt agaacggcaa cttttagata attcttgtgg cataagttct
      661 ggggtattta aataaaatat aagacaggct gtctatctta cagacagcct ttttataata
      721 acagaaagaa tatatatcat acttaacgga agagataata tgaaaacagt acccgtataa
      781 acagcaagca atcccatttt tccaggtgta tcagcaaaaa aaacgctgtt ccagaaatca
      841 ggccgggtaa atttcagagc tgtatcttca atataccatt ttgcaaccgg atacaaaacc
      901 attccgcaga gagaaataca ccagaacagt aatctgtatt taaaatcata atcccatgac
      961 atatacagca tatatcctcc cgtcacccat ccccaccaca tattattaaa ataatatttt
     1021 ctgttcatct gccagttttc ctttcttttg cacttttgtc agtgtactga tgcatgacaa
     1081 cataaccaca gcatgataat aataatcaat aacaataagc tgtgtcacgt ttacatcatg
     1141 gaacggaggt gctcctgtga cttttgagca gaaaaaagcc cgggccattg ccctgatgga
     1201 cagcaaaaag atgtggcgga gcaattatgc ccctcctctt ctgcgtattc tctggcggct
     1261 gggtatccgg cttccgccat taccctttat gccgttctgg caggtcacgg tgctgacggg
     1321 gggcttatgg ggcatttcct ggggatgtgc aatgtggttt atctactggg ggccgtcagg
     1381 aatggtggca ggtgaggcca ttatta
//
LOCUS       DAZPAC010000457         1404 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZPAC010000457 DAZPAC010000000
VERSION     DAZPAC010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.468"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            48..123
                     /locus_tag="ACHUPM_004710"
     tRNA            48..123
                     /locus_tag="ACHUPM_004710"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:81..83,aa:Lys,seq:ttt)
     gene            148..279
                     /locus_tag="ACHUPM_004711"
     ncRNA           148..279
                     /ncRNA_class="other"
                     /locus_tag="ACHUPM_004711"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            528..>1404
                     /gene="nadA"
                     /locus_tag="ACHUPM_004712"
     CDS             528..>1404
                     /gene="nadA"
                     /locus_tag="ACHUPM_004712"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR0872240.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT          381 a          342 c          344 g          337 t
ORIGIN      
        1 tgcacgaccc accatcctga atgattaaag cagcataatc ccgcaagggg tcgttagctc
       61 agttggtaga gcagttgact tttaatcaat tggtcgcagg ttcgaatcct gcacgaccca
      121 ccaatgtaaa aaagcgccct aaaggcgctt ttttgctatc tgagatattc aaagattcga
      181 acctgcagca ggttcgagtt gagcgcagcg aaacaacgga gccgctcgcg gcgacggccc
      241 gaagggcgag cgaagcgagt catcctgcac gacccaccaa tgtaaaaaag cgccctaaag
      301 gcgctttttt gctattcagg catcctcaat ttcactttgt aaacctgatg acatcgtcag
      361 agcttactgt gcaagcaact ctacgtcggt ggaattaggc gtaattcgag tgacttttcc
      421 ccaccattcg actatcttgt ttagcatata aaacaaatta cgccgatagt agcgaatatt
      481 acgctaatgt cggttttaac gttaagcctg taaaacgaga tggtaagatg agcgtgatgt
      541 ttgatccaga cacggcgatt tatcctttcc ccccgaagcc gacgccgtta agcattgatg
      601 aaaaagcgta ttaccgcgag aagataaaac gtctgctaaa agaacgtaat gcggtgatgg
      661 ttgcccacta ctataccgac cccgaaattc aacaactggc agaagaaacc ggtggctgta
      721 tttctgattc tctggaaatg gcgcgcttcg gtgcaaagca tcccgcttct acgttgttag
      781 tcgctggggt gagatttatg ggagaaaccg ccaaaattct cagtccggaa aaaacaattc
      841 tgatgctgac acttcaggct gaatgttcac tggatctcgg ctgccctgtt gaagaattta
      901 acgcattttg cgatgcccat cccgatcata ctgtcgtcgt ctacgccaac acttctgctg
      961 cggtaaaagc gcgcgcagat tgggtggtaa cttcaagcat tgccgtcgaa cttattgatc
     1021 atctggatag ttttggtgaa aaaatcatct gggcacccga caaacatctg gggcgttacg
     1081 tgcaaaaaca gacgggcgca gacattttat gctggcaggg tgcctgtatt gtgcatgatg
     1141 aatttaagac tcaggcgtta acccgcttgc aagaagaata cccggatgct gccatactgg
     1201 tgcatccaga atcaccacaa gctattgtcg atatggcgga tgcggtcggt tccaccagtc
     1261 aactgatcgc tgctgcgaaa acattgccac atcagcggct tatcgtggca accgatcggg
     1321 gcattttcta caaaatgcag caggcggtgc cagataaaga gttactggaa gcaccaaccg
     1381 caggtgaggg tgcaacctgc cgca
//
LOCUS       DAZPAC010000458         1401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZPAC010000458 DAZPAC010000000
VERSION     DAZPAC010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.469"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..228)
                     /locus_tag="ACHUPM_004713"
     CDS             complement(<1..228)
                     /locus_tag="ACHUPM_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFR0872241.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            409..561
                     /locus_tag="ACHUPM_004714"
                     /pseudo
     CDS             409..561
                     /locus_tag="ACHUPM_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            1187..>1401
                     /locus_tag="ACHUPM_004715"
     CDS             1187..>1401
                     /locus_tag="ACHUPM_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872242.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     ILNLRSIGIGLSFGFCQILGRYTMSLG"
BASE COUNT          446 a          205 c          271 g          479 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac atttttacat tgttttatat atataattgt tttgaatata tattacctga
     1021 attatgtggt ttgcatatat atatgcaata tagcagtctt tgcattgata actatacagt
     1081 agtaatttag agtgtgtaag tttttattga tggggtatat tttaattaaa tataattgaa
     1141 atgtattttt attctataaa caatatcttg ggtgaatgga ttctatatgg gaaatggtaa
     1201 cttttggcag ttgttcttgg gtaaagaacc ttacagcaaa aaatctgatt ttgttaaggc
     1261 atggttattg tggtgggcta ttaccatcct tgttctattt attttaagcc tgactacgat
     1321 attaaattta agaagtatag gtatagggtt aagttttggg ttttgtcaaa tccttggtag
     1381 atatacgatg tctctaggat g
//
LOCUS       DAZPAC010000459         1392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZPAC010000459 DAZPAC010000000
VERSION     DAZPAC010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.470"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..459
                     /locus_tag="ACHUPM_004716"
     CDS             <1..459
                     /locus_tag="ACHUPM_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0872243.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="ACHUPM_004717"
     CDS             477..1115
                     /locus_tag="ACHUPM_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0872244.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          307 g          359 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac ac
//
LOCUS       DAZPAC010000460         1380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZPAC010000460 DAZPAC010000000
VERSION     DAZPAC010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.471"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            78..>1380
                     /gene="norR"
                     /locus_tag="ACHUPM_004718"
     CDS             78..>1380
                     /gene="norR"
                     /locus_tag="ACHUPM_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR0872245.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHF"
BASE COUNT          285 a          317 c          448 g          330 t
ORIGIN      
        1 taatgagtag tcaaaatgac tatcaatcaa atggtcgata tgacaatatc tatagtcaaa
       61 ttgacagtga ggcaaagatg agtttttccg ttgatgtgct ggcgaatatc gccatcgaat
      121 tgcagcgtgg gattggtcat caggatcgtt ttcagcgcct gatcaccacg ctacgtcagg
      181 tgctggagtg cgatgcgtct gcgttgctac gttacgattc gcggcagttt attccgcttg
      241 ccatcgacgg tctggcaaag gatgtactcg gtagacgctt tgcgctggaa gggcatccac
      301 ggctggaagc gattgcccgc gccggggatg tggtgcgctt tcccgcagac agcgaattgc
      361 ccgatcccta tgacggtttg attcctgggc aggagagtct gaaggttcac gcctgcgttg
      421 gtctgccatt gtttgccggg caaaacctga tcggcgcact gacgctcgac gggatgcagc
      481 ccgatcagtt cgatgttttc agcgacgaag agctacggct gattgctgcg ctggcggcgg
      541 gagcgttaag caatgcgttg ctgattgaac aactggaaag ccagaatatg atgccaggcg
      601 atgccacgcc gtttgaagcg gtgaaacaga cgcagatgat tggcttgtcc cctggcatga
      661 cgcaactgaa aaaagagatt gagattgtgg cggcgtccga tctcaacgtc ctgatcagcg
      721 gtgagacggg aaccggtaag gagctggtgg cgaaagcgat tcatgaagcc tcgccacggg
      781 cggtgaatcc gctggtctat ctcaactgtg ctgcactgcc ggaaagtgtg gcggaaagtg
      841 agttgttcgg gcatgtgaaa ggagcgttta ctggcgctat cagtaaccgc agcgggaagt
      901 ttgaaatggc ggataacggc actctgtttc tggatgagat cggcgagttg tcgttggcat
      961 tgcaggccaa gctgctgagg gtgttgcagt atggcgatat tcagcgcgtt ggcgatgacc
     1021 gtagtttgcg ggtcgatgtg cgcgtgctgg cggcgactaa ccgcgactta cgcgaagagg
     1081 tgatggcagg gcgatttcgc gctgacttgt ttcatcgcct gagcgtgttt ccactttcgg
     1141 tgccgccgct gcgtgagcgg ggcgatgatg tcattctgct ggcggggtat ttctgcgagc
     1201 agtgtcgttt gcggctgggg ctctcccgcg tggtattaag tgccggagcg cgaaatttac
     1261 tgcaacacta tcgttttccg gggaacgtgc gcgaactgga acatgctatt catcgggcgg
     1321 tagtgctggc gagagccacc cgcaacggcg atgaagtgat tcttgaggcg caacattttg
//
LOCUS       DAZPAC010000461         1379 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZPAC010000461 DAZPAC010000000
VERSION     DAZPAC010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.472"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..983
                     /locus_tag="ACHUPM_004719"
     CDS             <1..983
                     /locus_tag="ACHUPM_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0872246.1"
                     /translation="VVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            998..>1379
                     /locus_tag="ACHUPM_004720"
     CDS             998..>1379
                     /locus_tag="ACHUPM_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR0872247.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLG"
BASE COUNT          359 a          315 c          365 g          340 t
ORIGIN      
        1 tagtggtggg tgttccggtt ccggacaata ttcacccgat gatcaaagac gaagcaggag
       61 tctggtcgtg gcgcacgcct gtcctgaaag gcaacctgta tgagtatttt ttcaatgttg
      121 atggtgtacg cagcattgat ataggcaccg caatgactaa gcctcagcgc caggttaact
      181 ccagtatggt tctggtgcca ggcagttatc tggatacgcg ttctgttgcg catggtgatt
      241 tgatcgccat aacttaccac tccaacgcat tgcaatctga acgtcagatg tatgtctgga
      301 ccccgccagg atacaccggc atgggcgagc ctttgccagt gctctatttc tatcacggtt
      361 ttggtgatac cggacgttcc gctatcgatc aggggcgtat cccgcaaatc atggataacc
      421 tgctggctga agggaaaatt aaaccgatgc tggtggtgat cccggatacc gaaaccgatg
      481 cgaagggcat tattcccgaa gatttcgtgc ctcaggaaag acgtaaagtc ttttatccgc
      541 tgaatgctaa agcggcagat cgcgaactga tgaacgatat tatcccgctg attagcaagc
      601 gttttaatgt ccgtaaagat gccgatggtc gcgcgctggc agggctttca caaggcgggt
      661 accaggcgct ggtttccgga atgaatcatc tggaaagctt tggctggctg gccacattca
      721 gtggtgttac cacgacaacc gtaccggatg aaggtgtcgc ggcccggctg aacgatccgg
      781 cagctattaa ccagcaacta cgtaatttta ctgtggtcgt tggtgataaa gatgtcgtaa
      841 ccggcaagga tatcgccggg ctgaaaactg agcttgagca gaaaaaaatt aagtttgatt
      901 accaggaata tcccggcctg aaccatgaaa tggatgtctg gcggcctgcc tatgcagcct
      961 tcgtacagaa attatttaaa taaaataagg cattaagatg aaattaatca ttaccgaaga
     1021 ttaccaggaa atgagccgtg tcgcggcaca ccatctgctt ggttatatgt cgaagacgcg
     1081 tcgtgttaac ctggcaatta ccgccggtag cacgcccaaa ggcatgtatg aatacctcac
     1141 caccctggtt aaaggtaagc cctggtacga taactgctat ttctataatt tcgatgaaat
     1201 tccctttcgc ggcaaagagg gagaaggcgt aacgattacc aatctgcgta atctgttttt
     1261 cacccctgcg gggatcaaag aagagaatat ccagaagctc actattgata actaccgcga
     1321 gcatgatcag aaactggcgc gtgaaggcgg cctggatttg gtggtgctgg gattaggtg
//
LOCUS       DAZPAC010000462         1376 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZPAC010000462 DAZPAC010000000
VERSION     DAZPAC010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.473"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>1376
                     /gene="traD"
                     /locus_tag="ACHUPM_004721"
     CDS             <1..>1376
                     /gene="traD"
                     /locus_tag="ACHUPM_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFR0872248.1"
                     /translation="GRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHG
                     TVSTGKSEVIRRLANYARKRGDMVVIYDRSCEFVKSYYDPSIDKILNPLDARCAAWDL
                     WKECLTQPDFDNVANTLIPMGTKEDPFWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTL
                     LSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKAIRYLQGIEHNGEPFTI
                     RDWMRGVREDQKNGWLFISSNADTHASLKPVVSMWLSIAIRGLLAMGENRNRRVWFFC
                     DELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVLNTRAFF
                     RSPSHQIAEFAAGEIGEKEHLKASLQYSYGADPVRDGVSTGKEMERQTLVSYSDIQSL
                     PDLTCYVTLPGPYPAVKLSLKYQTRPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQ
                     MASLFEPE"
BASE COUNT          324 a          349 c          385 g          318 t
ORIGIN      
        1 gtggtcgtca gctgacagac aatccgaaag acgttgcccg gatgctgaaa aaagacggca
       61 aggactccga tatccggatt ggcgatctgc cgattatccg ggattccgaa atccagaact
      121 tctgtctgca cggaacagtg tcgacgggta agtcagaggt tattcgacgt cttgccaact
      181 atgcccgtaa acgtggcgac atggtggtga tttatgaccg ttcgtgtgag tttgttaaaa
      241 gttactatga tccctccatc gataagatcc tgaatccgct ggatgcccgc tgtgccgcct
      301 gggatttgtg gaaggagtgc ctgacacagc cggattttga taatgtcgcg aacacgctga
      361 tcccgatggg aacgaaagag gacccgttct ggcagggatc aggacgtacc atttttgcgg
      421 aagcggcgta tctgatgcgt aatgacccca accgcagcta cagcaaactg gtggacacac
      481 tgctttccat caaaatcgaa aaactgcgta ccttcctgcg taattcaccg gcggccaacc
      541 tggtggaaga gaaaattgag aaaacggcga tttccatccg tgctgtgctg accaactacg
      601 tgaaagccat ccgttacctg caggggattg agcataacgg tgaacccttc accatacgtg
      661 actggatgcg gggtgtccgg gaagatcaga aaaacggctg gctgtttatc tcgtcaaatg
      721 ccgacactca tgcctccctg aagccggtgg tttccatgtg gctgtccatt gccattcgtg
      781 gtctgctggc aatgggagaa aaccgtaacc gtcgtgtgtg gtttttctgt gatgagttac
      841 ccacgttaca caaactgccg gacctggtgg agatcctgcc ggaagcccgt aagttcggag
      901 gctgttatgt gtttggtatc cagtcctatg cccagctgga agatatctac ggtgagaaag
      961 cggcggccac gctgtttgat gtgctgaata cccgtgcctt tttccgctcc ccgagccatc
     1021 agattgcaga gtttgctgcc ggtgaaattg gtgagaaaga gcacctgaaa gccagtctgc
     1081 agtattccta cggtgctgac cccgtgcgtg acggggtatc gaccggtaag gagatggagc
     1141 gccagacgct ggtcagttat tccgacattc agtctctgcc ggatctgacc tgttatgtca
     1201 ctctgcccgg accgtatccg gcagtaaaac tctcactgaa atatcagaca cgaccgaagg
     1261 tcgctccgga gtttattccg cgtgacatca acccggaaat ggagaaccgt ctgagtgccg
     1321 tacttgctgc aagggaagca gaaggtcgcc agatggccag cctcttcgaa ccggag
//
LOCUS       DAZPAC010000463         1372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZPAC010000463 DAZPAC010000000
VERSION     DAZPAC010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.474"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(186..305)
                     /locus_tag="ACHUPM_004722"
                     /pseudo
     CDS             complement(186..305)
                     /locus_tag="ACHUPM_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            549..921
                     /locus_tag="ACHUPM_004723"
                     /pseudo
     CDS             549..921
                     /locus_tag="ACHUPM_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          394 a          313 c          309 g          356 t
ORIGIN      
        1 tatgcaagct tctttgtata ctatgattta aaaaaacgga acaaaaatgc aattatattc
       61 tggcattgtt taatgctgtt tgtctttttt attcattatt tcaatgaaag aatttcaact
      121 acaaagttaa ttgatcctac ctacataata tagacacagt acctaaagta ccgtggagat
      181 catccgggga agccccgggt ctgataccag tgcaggtaga ccgctatctt gttccggtaa
      241 tgttctgccg ttctttacgg catcacctct agcagaaaca tcgcgtaact gcgccaagta
      301 tgatgaactt ctcttaccgc tgcgatctgg ttcaacaata tgcagatggt acacaacctc
      361 tttaaccagc ccatccggat tattcagagc cgcttccgca atccggaata aaagtgctgt
      421 tttaccgtga accctctgaa aatcagcgag cagtgtttta accagccgac gttcagagcg
      481 tactgacaga cggtggacaa tttgaataaa cagttcaatc agaccgtaga tgctctcccg
      541 ttggcgacat gctgggagcg gtggaacacc gcttcggcaa cgagcttccg gcgtctccag
      601 tgaagtgact gacggataat ggttcatgct accgggctaa tgaaacacgc cagttcaccc
      661 ggatgttggg actttgttga accgaagaac acggcggtgc ggagtccgga gagtaacaga
      721 atagcaaaga gcttcgtgaa aacaataaag tgtgactaca tcagtatcat gcccaaacct
      781 gacgggttaa cggcagcaaa gaaccttgca gaggcattcg agcattataa cgaatagcat
      841 ccgcatagtg cgctggatta tcgctcgcca cgggaatatc tgcggcagcg ggccaatgat
      901 aacaggtgtc tggaaatata ggggcaaagc cacccacgaa cgatccagca tataatcaag
      961 aaattagatt tcatttaaca taagatagat tatgtgcacc aagaggcgtt tccttaattt
     1021 ctgaatttac aggctctttt gctctctttg ctatgctgtg gatttggaaa gatagagtgc
     1081 tggctgatta tttaggtatt ttcatcagca tgtccctgat agcactgttc catcagcgct
     1141 ttcaggcgat aatagtagac cttgggtagc ctctgcccat gtgtaacttg ataccccgag
     1201 aacagccacg atgatctaag cggttcttat ttctccggtc tcagggtcgg taatgccaac
     1261 agtaagtcca cagtaatcaa cgaagagttt tttgaaccgc cccggaaatc ctggagacta
     1321 aacgccctga gaaagaggta aacagaatga ctaaaaatac acgtttttcc cc
//
LOCUS       DAZPAC010000464         1370 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZPAC010000464 DAZPAC010000000
VERSION     DAZPAC010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.475"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..185)
                     /locus_tag="ACHUPM_004724"
     CDS             complement(<1..185)
                     /locus_tag="ACHUPM_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR0872249.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
     gene            complement(230..1216)
                     /locus_tag="ACHUPM_004725"
     CDS             complement(230..1216)
                     /locus_tag="ACHUPM_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR0872250.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT          379 a          301 c          276 g          414 t
ORIGIN      
        1 gcacctaata accctaccca gatcatcaaa aatcgtgcca gctcatcggt taatgtactt
       61 ggctggttaa gtacgtagcg gctaaatact tgccagacta cgcatacaac cagagcaagc
      121 atgacggcga cagaaaacgc ggcaatgacg cggtctaccg caagtttcag gcggttaaat
      181 atcattgtaa gcatcttgga aaatcagggc cagagactgg cccggaatat tattgagcag
      241 cgttgtcgaa cttctctaac aaagcagctt gttttggatc ttttttgaag tcgtcaaaca
      301 gaggctgaac tgcggcacgg aatggtgctt tatccacttt tacaatttca ccccccatag
      361 ctttcgcctg agcacgtgtg tcagcgtcaa ttttttccca caatttttgt tgataagctt
      421 cggatttctt cgctgctgtt tcgaggattt gtttctgttc tggggttaat ttattccatg
      481 ttttggtgga gataaccaaa aagtcaggaa ttgaggcgtg ttcatcttca gaaaaaactt
      541 tggcaatttc aatatgtcga gtctgcaccc aggaaggcac gttattttct gcgccatcta
      601 ctacaccttg ctgcatcgca gtataaactt cgccaaatga aattggagtg ggtgaaccac
      661 ccatcagttc aatcattttg atggtggtcg gacttggctg aacacgaatt ttcagccctt
      721 taagatcttc tggcttagta atcggttttt tcgcatagaa actgcgtgta cccgctacat
      781 aagctgataa agcaaaaaag cctttgtctt ttgttgagtc cataatttct ttaccaactt
      841 caccaaaaac aactttgttg aagtgagcct gatctttaaa taagaacggc aaattataga
      901 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg
      961 cgccattttg caacaattcc atagtttctc gcgcattacc catctggctg ctgggataaa
     1021 tacggatcac catattacct tctgacagct gttttacctc tttcgccagc tcttcaaaac
     1081 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgtc actttttctg
     1141 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
     1201 atgacctcat tttcataaaa aaactccctg ttgaaggatg gaaattggtt gatatctaat
     1261 tgtgcttatc agtgctttgt gcgacaaatt ggttaaccta tctgacaact tgtctatgtt
     1321 tatcacaact tgaaataaag tcagaaatta aatattaaat ttgtgcgcca
//
LOCUS       DAZPAC010000465         1347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZPAC010000465 DAZPAC010000000
VERSION     DAZPAC010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.476"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1139
                     /locus_tag="ACHUPM_004726"
     CDS             <1..1139
                     /locus_tag="ACHUPM_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR0872251.1"
                     /translation="TNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSN
                     DVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLS
                     SYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALL
                     CLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHD
                     VLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVD
                     IIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQ
                     MTISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT          367 a          287 c          268 g          425 t
ORIGIN      
        1 tcactaacaa acaaatttta ccaacactca ttttaagcct tgcattttta agtggcctta
       61 tctatttagt tgaaaccatt gtaattatcc ataaaccaat taacggcagt acactgatcc
      121 agacaaagtc gaatgatgtt tctattttct atattttccg ccaactcagt tttatttgtt
      181 taacctcgct ggcactcttt tgttatggaa aagacaacat ccttgacaac aataagaaaa
      241 aaacgggaat cctgttgctg gcgctgattc cttttttagt ttttcccctt ctggcacaca
      301 atctgagcag ttataacgct gactattctt tgtatgtcgt cgactactgt ccggacaacc
      361 atactgcgac ctggggaatc aactatacaa aaatattggt ttgtctgtgg gcatttttac
      421 tgttctttat tatcatgcgt acacgattag ccagcgaact atggccgtta atagcattat
      481 tatgtctggc atcgctatgc tgcaacttac ttctactgac tctggatgag tataattaca
      541 ccatctggta tatcagtcgc gggattgaag tttccagtaa actgtttgtt gtgtcttttc
      601 tgatttataa catttttcag aaactgcaac tctccagcaa actggcagtt catgatgtgc
      661 tgaccaatat ttataatcgg cgctactttt tcaacagcgt agagtcatta ttgtcgagac
      721 ctgttgttaa ggacttctgt gtcatgctgg ttgatattaa tcagttcaaa cgcatcaatg
      781 cccaatgggg acatcgtgtg ggtgataaag tgctggtttc aattgtcgat attatccagc
      841 aaagcatccg ccccgatgat attttagcgc gactggaggg tgaggtgttt ggcttgctat
      901 ttaccgaact caatagtgcc caggcaaaaa tcattgcgga acgtatgcgt aaaaatgtcg
      961 aactcctgac cggctttagt aacagatatg atgttcctga acaaatgacc atcagtattg
     1021 gcacggtttt ttcaacgggt gacacgcgta atatctcgct tgtcatgacg gaagcagata
     1081 aagccttacg cgaagcgaaa agcgaggggg gcaacaaagt gattattcat catatttaag
     1141 tgcaaaaata ttcagagcca tgctttttgc gtggcttttg catacaattt attactatta
     1201 cccctaaatt tcccctcccc agcggcgcag agatgagtat aattagcgcc cctgtgccag
     1261 gccgcaatcg aactttatct ggttttctcg tttcactgaa ccgaaggagt gccatttatc
     1321 atgaaattgc accatagaat gctccgg
//
LOCUS       DAZPAC010000466         1346 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZPAC010000466 DAZPAC010000000
VERSION     DAZPAC010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.477"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..611
                     /locus_tag="ACHUPM_004727"
     CDS             <1..611
                     /locus_tag="ACHUPM_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFR0872252.1"
                     /translation="KNQEERKTPIIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAESAP
                     AGINSSAVETHIPEAPARKKKKKKKKKRHRFPRPSHWTREYTHECVEKIKILFPHLRA
                     EGGGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRY
                     GLDGHPKGQVSESEAQSAGRRLVTLEQKLLRMQAQQENISGQ"
     gene            667..1176
                     /locus_tag="ACHUPM_004728"
     CDS             667..1176
                     /locus_tag="ACHUPM_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872253.1"
                     /translation="MSKSGAEKIQFILNNHEQAYRRLKPVYTFLELASWISVVLLIIY
                     LSGKNHVLTAAFLCFAWTAFWVLFLLHYFPKVFFILDRQSLLNLMEATKDSPDVKQEL
                     LNRLFSGKRMTGRDEMDICRLLRKNEEEKIRQSELNIIRNFIDEQQLTNSDIPKKSTV
                     PNEDKKGIR"
BASE COUNT          437 a          283 c          301 g          325 t
ORIGIN      
        1 gaaaaaatca ggaagaaagg aaaactccca tcattgtggt gaaaaaacgt cgtacttttt
       61 cgccaccgtc cttatccgaa aaaacagata ttatcgcacc tgtatttact gaacagacgg
      121 cagaatcagc ccccgccgga ataaattcat ctgccgtcga aacacatatc cccgaagcgc
      181 cagccaggaa gaagaagaag aagaagaaaa agaaaagaca ccgttttcca cgtccctccc
      241 actggaccag agagtatact cacgagtgcg tggaaaaaat aaagatcctg tttcctcatc
      301 ttcgtgctga aggtggtgga tttattcccc tgaaaattgg cataaacaat gatatttcag
      361 catttctggc agaacacccg gagacagaac tgaccatgga cgaatggctc tgtgcagtgt
      421 cctgcatcac ctcacgccgt gtatatctgc agcggacagc tgtcgccggc gtcccacgtt
      481 atggtctgga tggtcacccc aaagggcagg tctcagaatc tgaagcacag agtgccggtc
      541 gcagactggt gacattagaa caaaagttgc tcaggatgca ggcacagcag gagaatattt
      601 ctgggcaata aacactcctg ataacagctg gacattacgt atcagaaaaa tgggaagaga
      661 attattatga gtaaatcagg tgcagaaaaa attcaattca tcctgaataa tcatgaacag
      721 gcatacagac gcctgaagcc tgtatatact tttttagaac tggcatcctg gatttcggtt
      781 gttctgctga ttatatacct atcaggcaag aatcacgtgc ttacagcagc ttttctctgc
      841 tttgcatgga cagcattctg ggttcttttc cttttacatt atttcccaaa agttttcttt
      901 attctcgatc gtcagagtct gctcaacctc atggaggcca ccaaagactc accagatgta
      961 aagcaggagc tgcttaaccg ccttttttcc ggaaaaagaa tgaccgggcg ggatgaaatg
     1021 gatatctgcc gtcttctgag aaaaaatgaa gaagaaaaaa tccgccagag tgaactgaat
     1081 atcatcagga attttattga tgaacagcag ttaacgaata gtgatattcc gaagaaaagt
     1141 acggtcccaa atgaagataa aaaaggcata agatgatatg aaattaataa ccaggcaagc
     1201 agaggatggc gtggtttcat cctaagattg ataggagtta acctgtaaga taaaaggacc
     1261 gtgtttacac gacgtctctg aagggcttcg tttgggaaac ggacaatatc taactgacga
     1321 ggaaattaaa taagggttta aaaatt
//
LOCUS       DAZPAC010000467         1341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZPAC010000467 DAZPAC010000000
VERSION     DAZPAC010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.478"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHUPM_004729"
     CDS             <1..184
                     /locus_tag="ACHUPM_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872254.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="ACHUPM_004730"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="ACHUPM_004730"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR0872255.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAZPAC010000468         1334 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZPAC010000468 DAZPAC010000000
VERSION     DAZPAC010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.479"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            26..709
                     /locus_tag="ACHUPM_004731"
     CDS             26..709
                     /locus_tag="ACHUPM_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR0872256.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWHRIDRFMAAWKRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            710..931
                     /locus_tag="ACHUPM_004732"
     CDS             710..931
                     /locus_tag="ACHUPM_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872257.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            861..>1334
                     /locus_tag="ACHUPM_004733"
     CDS             861..>1334
                     /locus_tag="ACHUPM_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR0872258.1"
                     /translation="MAPIACSWRRIPKFWNWIPASKIEKETRMYGTCETLCRALAAKY
                     PGDTPLMLVIWSPEEIQALADGMDISLSDHEIRTVLARLEDIPEDQRTESGISSGAAM
                     EIINNVRENRQVTVPAELLASLIQTAEQALWKREWAARDNGLAVPECVTRRQAVVN"
BASE COUNT          297 a          382 c          380 g          275 t
ORIGIN      
        1 cctctgtaag agaaggagcg ttgttatgtc ccgttttgtc ctcggtaact gcatcgatgt
       61 tatggcccgt attcctgata acgccattga tttcatactg actgacccgc cgtacctcgt
      121 cggttttcgt gaccgttccg ggcgcaccat cgctggcgat aaaaccgatg aatggctgca
      181 accggcctgt aatgaaatgt accgcgtact caaaaaagac gcgctgatgg tcagcttcta
      241 cggctggcac cgtattgacc gctttatggc ggcctggaaa cgcgccggat tcagcgtggc
      301 agggcacctc gtgttcacca aaaactacac atccaaatct gcgtatgtcg cgtaccgcca
      361 cgaatgcgct tacatcctgg caaagggcag tccggcccgg ccccgtaacc ctctgccgga
      421 tgtgctgggc tggaaatatt caggcaatcg tcatcacccg acggaaaagc ccgttaccag
      481 cctgcaacca ctgattgaga gcttcacaca tccgaacgcg attgtgctgg acccgtttgc
      541 aggcagcggc tcaacctgcg tcgccgccct ccagtccgga cgccggtata tcggtatcga
      601 gctgcttgaa cagtatcacc gtgccggaca gcaacgactg gccgccgtgc aacgggccat
      661 gcagcagggg gccgcgaatg ataactggtt tgaaccggag gctgcgtaaa tgaactatgc
      721 cggacacgaa aaacttcgcg ccgacgtggc ggaggtggcc aacaccatgt gtgaccttcg
      781 ggccaggctg aacgatatgg agcatcgctg tcgctttgat tccgatgtgc ttgttgaacg
      841 cctgacacgc cagacgctgt atcgcgccaa tcgcctgttc atggaggcgt ataccgaaat
      901 tctggaactg gattcctgct tcaaagattg agaaggagac gagaatgtac ggaacatgcg
      961 aaacgctctg ccgggcgctg gcagcaaagt atccgggaga tacgccgctg atgctggtta
     1021 tctggtcccc ggaagaaatt caggccctcg ctgacggaat ggatatttcc ctgtccgatc
     1081 atgaaatcag gactgtcctg gcgcgcctgg aggacatccc ggaagaccag cggactgaat
     1141 ccggtatttc ttccggtgcg gcgatggaga tcatcaataa cgtgagagaa aaccgccagg
     1201 tgaccgtccc tgctgaactg ctggcgtccc tgattcagac cgccgaacag gcactgtgga
     1261 aacgtgagtg ggccgcccgt gataacggcc tcgccgtccc ggaatgcgtc acccgccgac
     1321 aggcggtggt taat
//
LOCUS       DAZPAC010000469         1316 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZPAC010000469 DAZPAC010000000
VERSION     DAZPAC010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.480"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="ACHUPM_004734"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="ACHUPM_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
BASE COUNT          383 a          273 c          278 g          382 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgtcg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttat
//
LOCUS       DAZPAC010000470         1294 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZPAC010000470 DAZPAC010000000
VERSION     DAZPAC010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.482"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..1112
                     /locus_tag="ACHUPM_004735"
                     /pseudo
     CDS             1..1112
                     /locus_tag="ACHUPM_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1090..>1294
                     /locus_tag="ACHUPM_004736"
     CDS             1090..>1294
                     /locus_tag="ACHUPM_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872259.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIK"
BASE COUNT          355 a          262 c          399 g          278 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacacc actggggtta gaacgagtta
      661 taagtgtata tgggcctccc gctccagacc gggcaggtgc tgaaactcct cttgttctta
      721 ctgatatgac tggaggagtg actatttatt atgatcctga aacaggtgat tcaatgactt
      781 ttgatagtag caatagaatt gatcgtcgtt ctcaacgtgg tgctggtgat ccatatacag
      841 gtgaagtcgt tggttgtgag acaaatggat caggaatatc ggcagcatat ggcacaacga
      901 agatctatac tacagatact agagcaagat ggctacatgg tgggggatca tcgttgcgcg
      961 atccttatgc tcctcgacaa ggctggaaac caactatggg atgtacccgt gcacagaatg
     1021 aagatgtaga tgagttgtgt aagaaagtga caagttggat gtattcgcat cctggagaga
     1081 gaattagata tgaacgtttt aagacacgtt aatgcgatta acatgggagt gtgcattcta
     1141 attttggtta ttggatgtgc aaatgctaaa gttcttaaac gatgtatatt acccgctcct
     1201 gatggtggcg tagaaaaact atcaaaaccc aacttacatg gagtaattaa aaaagttgat
     1261 ttgaacacaa atgaagcgga aattaaaata aaag
//
LOCUS       DAZPAC010000471         1293 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZPAC010000471 DAZPAC010000000
VERSION     DAZPAC010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.483"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..401)
                     /locus_tag="ACHUPM_004737"
     CDS             complement(<1..401)
                     /locus_tag="ACHUPM_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0872260.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVK"
     gene            453..1058
                     /gene="wrbA"
                     /locus_tag="ACHUPM_004738"
     CDS             453..1058
                     /gene="wrbA"
                     /locus_tag="ACHUPM_004738"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0872261.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            1079..>1293
                     /locus_tag="ACHUPM_004739"
     CDS             1079..>1293
                     /locus_tag="ACHUPM_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR0872262.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIER"
BASE COUNT          349 a          310 c          320 g          314 t
ORIGIN      
        1 aacttcacag ggtctgttaa ggctgacttg tcgtagagtt caccatcacc cagagtcagt
       61 atagagaccc ccttcctcag tatctgcctg acatcatcta tagctgtgtc gatatcagcc
      121 ctactgaagc ggtcaaagtt ctctacaaca atcaaggaac cttctggaat agccccagac
      181 ataacatttt caaacatacg tctgaagtca gaaccttgat tcttatgttg ctggtgaaaa
      241 ccagatttac ccttatcaac atatctgtga gcaggaagaa ggtaataatc gtttttgtgc
      301 ttagagtaga aagcatcaat gaggtttgtt tgccgtctta aggagtctcc tgagtcttgc
      361 tctttagtac tccacctgat gtatgtcact agcagcttca tatcttcgga ctatgcttat
      421 ctaagtggtt cattgaacag gagaacaatg atatgagtgt taaggcaaga atactagtgc
      481 tttattattc catgtacgga catattgaaa cgatggcacg cgcagtcgct gagggtgcaa
      541 gcaaagtcga tggcgcagaa gttgtcgtta agcgtgtacc ggaaaccatg ccgccgcaat
      601 tatttgaaaa agcaggcggt aaaacgcaaa ctgcaccggt tgcaaccccg caagaactgg
      661 ccgattacga cgccattatt tttggtacac ctacccgctt tggcaacatg tccggtcaaa
      721 tgcgtacctt cctcgaccag acgggcggcc tgtgggcttc cggcgcacta tacggaaaac
      781 tggcgagcgt ctttagttcc accggtactg gcggcggtca ggaacaaact attacttcaa
      841 cctggacgac ccttgcgcat cacggcatgg tgattgtccc cattggctac gcagcgcagg
      901 aattatttga cgtttcacag gttcgcggcg gtacgccgta cggcgcaacc accatcgcag
      961 gcggtgacgg ctcacgccag ccaagccagg aagaactgtc tattgctcgt tatcaagggg
     1021 aatatgtcgc aggtctggca gttaaactta acggctaatc ttcaacagga ggatacgcat
     1081 gccaactcaa gaagcgaaag ctcatcacgt cggtgaatgg gcatctctgc gcaatacgtc
     1141 gccggaaata gccgaggcca ttttcgaagt cgccgggtac gacgaaaaaa tggcagaaaa
     1201 gatttgggaa gaaggtagcg atgaagtgtt agtcaaagcc tttgctaagg ccgataaaga
     1261 ttcgcttttt tggggcgaac agaccatcga acg
//
LOCUS       DAZPAC010000472         1291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZPAC010000472 DAZPAC010000000
VERSION     DAZPAC010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.484"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>1291
                     /gene="rhsC"
                     /locus_tag="ACHUPM_004740"
     CDS             <1..>1291
                     /gene="rhsC"
                     /locus_tag="ACHUPM_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HFR0872263.1"
                     /translation="LSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDS
                     LNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVT
                     GLITRITTPDGRASAFYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDITRY
                     RYDNPHSDLPCATEDATGSRKTMTWSRYGQLLTVTDCSGYVTRYDHDRFGQVTAVHRE
                     EGLSQYRAYDSRGQLIAVKDTQGHEMRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAI
                     CTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLT
                     GKLIRSEDEGLVTHWHYDEADRLTHRTVKGETAERWR"
BASE COUNT          309 a          377 c          400 g          205 t
ORIGIN      
        1 gtctgtctgc cgtgtggctg acgcacgacc cggaataccc ggagaattta cctgccgcgc
       61 cgctggtgcg ctatggctgg acgccgcgcg gcgaactggc ggcagtgtat gaccgcagca
      121 atacacaggt gcgcagcttt acttacgatg ataaataccg gggccggatg gtggcgcacc
      181 gtcacacggg ccggccggaa atccgttacc gttacgacag cgacgggcgg gtgacagaac
      241 agctaaaccc ggcaggctta agctacacgt atcagtatga gaaggaccgc atcaccatca
      301 ccgacagcct gaaccgccgt gaagtgctgc acacgcaggg tgaagccggg ctgaagcggg
      361 tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag tcagtttgat gcggtgggca
      421 ggctcagggc acagacggat gccgcaggcc ggacgactga gtacagcccg gatgtggtga
      481 cgggactcat cacgcgcatc accacgccgg atggcagggc atcggcgttt tactataacc
      541 accacagcca gttaacgtca gccaccgggc ctgacgggct ggaaatgcgc cgcaaatatg
      601 atgaatccgg ccgtctgatt caggaaaccg cccctgacgg cgatatcacc cgctaccgtt
      661 atgataatcc acacagtgac ttaccctgcg caacggaaga tgccaccggc agccggaaaa
      721 ccatgacatg gagccgttac ggtcagttgc tgaccgtcac tgactgttcc ggttatgtaa
      781 cccgttatga ccatgaccgt tttggtcagg tgacggcggt gcaccgcgag gaagggctga
      841 gccagtaccg cgcatacgac agccgtggac agttaattgc ggtgaaagac acgcagggcc
      901 atgaaatgcg gtatgaatac aacgccgccg gtgacctgac cgccgtcatt gccccggacg
      961 gcagcagaaa cgggacacag tacgatgcgt ggggaaaagc catctgtacc acgcagggcg
     1021 ggctgacgcg cagtatggaa tacgatgctg ccggacgggt catccgtctg accagtgaaa
     1081 acggcagcca caccaccttc cgttacgatg tactcgaccg gctgatacag gaaaccggct
     1141 ttgacggccg cacacagcgt tatcaccacg acctgaccgg caaacttatc cgcagcgagg
     1201 atgaggggct ggtcacccac tggcactatg acgaagcaga ccgcctcacg caccgcaccg
     1261 tgaagggtga aaccgcagag cggtggcggt a
//
LOCUS       DAZPAC010000473         1288 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZPAC010000473 DAZPAC010000000
VERSION     DAZPAC010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.485"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..154
                     /locus_tag="ACHUPM_004741"
     CDS             <1..154
                     /locus_tag="ACHUPM_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR0872264.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
     gene            258..1172
                     /locus_tag="ACHUPM_004742"
     CDS             258..1172
                     /locus_tag="ACHUPM_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0872265.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAADGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT          377 a          266 c          293 g          352 t
ORIGIN      
        1 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
       61 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      121 aagagctatc acggtgaatg gaaacgcaag ctagggaacg atcgaggcgc taatcaatgt
      181 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      241 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      301 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      361 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      421 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      481 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      541 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      601 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      661 ttggggcggc cgatggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt
      721 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
      781 ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
      841 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
      901 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
      961 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa
     1021 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1081 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt
     1141 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct
     1201 ccattcacga aaattgcgca gataattatt ttgtgaaggc tattatccta cacctgtaag
     1261 aaaatccgcg cacaattgaa gccagagt
//
LOCUS       DAZPAC010000474         1260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZPAC010000474 DAZPAC010000000
VERSION     DAZPAC010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.486"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(19..>1260)
                     /locus_tag="ACHUPM_004743"
     CDS             complement(19..>1260)
                     /locus_tag="ACHUPM_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0872266.1"
                     /translation="IKHIIIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAP
                     GWLELSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYPTINQRKVRIS
                     KTKKPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFY
                     AVQQLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNK
                     ESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTE
                     TLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEA
                     QIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDM
                     LIFGEPVTRIYPAGDMEEVDA"
BASE COUNT          332 a          297 c          227 g          404 t
ORIGIN      
        1 gaaccctaac aaaaaatact aagcgtccac ctcttccata tcaccagcag gataaatcct
       61 tgttactggt tcaccaaaaa taagcatatc atctaatgac tgcatctcat cggttgcttc
      121 ttcaagcgtt aaggtgtggt catctttacc atcaagagga aaaccacgaa tcatatttcc
      181 attgtgaaga tatatatcca ccagcttttc attaccagaa acaacaatct ctttcaccag
      241 acgcttaata ataatctggg cttcagttct cccctctaca gactccatat caagaccgct
      301 taaatccaag cttgatacag attttaccct cgccttttct cgtgataact gaactatcag
      361 ttcatctttt tcttcctgtt taacccgtag tgcttcttga atctgagtga tgttaggaag
      421 ctgtatcagc gtctcagtga gtttttccag ttcaccatca acggtgttta atcttctttg
      481 tagttcatct aatttagctg tatcggtagc ttcatcgtgt agctgtgaca gtttgctaaa
      541 cagacgacta caaagctgtg tatcaagtaa ctttctggac actgtaccgt agctacaacg
      601 ggactcctta ttaccattgc aacggtaagt accttgataa acgggtttac gtatccctgt
      661 aggagtcatt acaagcccac acttacaacg gataagcccc ttcagtatgt tcacatatag
      721 ccagtgttca ccgctggatg ttttcccctt ccctgtttcc tctaacagtt gctgtacagc
      781 gtaaaactta gagtctgtga caatctgagg atagaaccct tcaatttctt cacgtaacac
      841 tgttttacgg tcttctgtag aaatcttagc gggaaggtaa cctaatacac ttcgtgaagt
      901 caggatgtgt ttaacgctta atgctgacca gttgccatcc ggtttcttag tcttggatat
      961 tctgacctta cgctgattaa tggtcgggta accttgttcg tttagccatt tacagatagc
     1021 aaacatcgac ataccagaaa gacgcttgtc atagatgata ttcaccagtg acgctttatc
     1081 ttcatcaacg atgtagtatg aaccatcttc tgatagctct aaccatccgg gggcttgctt
     1141 acccataggc ttcttaagtt ctcttgcaag ctgtgtttta tggttccaga cctgagctat
     1201 acgcttttgc ttaacaagag attcttggtg tgctctctca gctatgatga tgtgcttaat
//
LOCUS       DAZPAC010000475         1254 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZPAC010000475 DAZPAC010000000
VERSION     DAZPAC010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.487"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            62..1156
                     /gene="ltrA"
                     /locus_tag="ACHUPM_004744"
     CDS             62..1156
                     /gene="ltrA"
                     /locus_tag="ACHUPM_004744"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR0872267.1"
                     /translation="MPHCADGAAATTGDNADGRTAVKSAKPFPVSKRQVWEAYKRVKA
                     NRGAAGIDGQTLAGFDENVTDNLYKLWNRMASGSYMPQAVRRVDIPKADGGVRPLGIP
                     AVSDRIAQMVVKQILEPVLEPLFHADSYGYRPGKSAHQAIAQARKRCWKFDWVVEVDI
                     KGFFDDIDHDLLLKTVQHHTQARWVVMYIERRLKAPVQMPDGAMLARGRGTPQGGVIS
                     PLLSNLFLHYAFDMWMQRQFPGVPFERYADDVVCHCHSQWQADALISGLRQRLAQCGL
                     QLHPQKTRIVYCKDADRRGDYPETSFDFLGYTFRPRLSMNRWGKTFVNFSPAMSARAG
                     KAIRQEVRRIAVTSPCTSWRICSMRKSEAG"
     gene            1126..>1254
                     /locus_tag="ACHUPM_004745"
     CDS             1126..>1254
                     /locus_tag="ACHUPM_004745"
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HFR0872268.1"
                     /translation="MFNAKIRGWIKYYGAFYKSALYLTLRQIDRKLVLWLPRKHKRL"
BASE COUNT          308 a          298 c          381 g          267 t
ORIGIN      
        1 tacagggacg gagtaaaccg tagtagcggt gaaggaccgt aatgggtccg gagcaaaggg
       61 cttgccccat tgtgctgatg gtgcggcggc aaccacgggc gacaatgccg atgggaggac
      121 tgctgtaaaa tcggcaaagc catttcctgt aagtaaacga caggtatggg aagcttataa
      181 acgagtaaaa gccaaccgtg gggcggcagg cattgacggg cagacactgg ccgggtttga
      241 cgagaatgtt acagacaatc tctacaaact gtggaacaga atggcttccg gcagttatat
      301 gccgcaggcg gtaaggcgtg tggacattcc caaagctgac ggtggtgtac gtccgctcgg
      361 tattccggcc gtgtcggacc ggatcgccca gatggtcgtt aagcagatac tcgagccggt
      421 actggaaccc ctgttccatg cagactcgta tggttatcga ccgggtaaat ccgcacacca
      481 ggcgattgcg caggcgagga aacgctgctg gaagtttgac tgggtggtgg aagtcgatat
      541 taaaggcttc tttgatgata tcgatcatga cctgctactg aagacagtgc agcatcatac
      601 tcaggcacgc tgggtggtga tgtacattga gcgcaggctg aaggcaccgg tgcaaatgcc
      661 ggatggagca atgctggcac ggggtcgagg aacgccgcag ggcggcgtaa tctcccccct
      721 gctctcaaat ctgttcctgc actatgcctt tgatatgtgg atgcaacgtc agtttcccgg
      781 cgttcccttc gaaaggtacg ccgatgatgt tgtatgccac tgtcacagcc agtggcaggc
      841 tgacgctctg ataagtggac tgaggcagcg gcttgcgcaa tgcgggctgc agctgcatcc
      901 gcaaaaaaca cgaatagttt actgtaagga tgctgaccga cggggggact acccggaaac
      961 gagcttcgac tttctgggct atacattccg gccgcgtctg tcgatgaatc gctggggtaa
     1021 gaccttcgtt aacttcagtc cggcgatgag tgccagagca ggaaaagcaa tccgtcagga
     1081 ggtgaggaga atcgcagtga caagtccttg tacgagctgg cgcatatgtt caatgcgaaa
     1141 atcagaggct ggataaagta ctacggtgct ttttataaat cagcactgta tctgacgctg
     1201 aggcaaatcg atcgcaaact cgtgttatgg ctgccccgta aacacaagcg actt
//
LOCUS       DAZPAC010000476         1253 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZPAC010000476 DAZPAC010000000
VERSION     DAZPAC010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.488"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..365
                     /locus_tag="ACHUPM_004746"
     CDS             <1..365
                     /locus_tag="ACHUPM_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0872269.1"
                     /translation="QEKLIWLRAQMKLTSLCWALKELAKDIWSRPWSEERRNDWQRWL
                     SLAANSDVPMMKNVAKTIGKRLYGILNAMRHGVSNGNAEALNSKIRLLRIKAKGYRNR
                     ERFKLGVMFHYGKLNMAF"
     gene            395..796
                     /locus_tag="ACHUPM_004747"
                     /pseudo
     CDS             395..796
                     /locus_tag="ACHUPM_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912930.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          361 a          266 c          324 g          302 t
ORIGIN      
        1 ggcaggaaaa gctgatatgg ctgcgtgcgc agatgaagct gacgagcctg tgctgggcgc
       61 tgaaagaact ggcaaaggat atctggagca gaccatggag cgaggaaaga cgcaacgact
      121 ggcagagatg gctgtcgctg gcggccaaca gtgacgtccc catgatgaag aatgtggcta
      181 aaacgatagg gaaaaggttg tacggtatcc tgaacgccat gcgccatggc gtatcaaacg
      241 gaaatgcgga agcactgaac agcaagatca ggctgctgag aataaaagcc aagggctacc
      301 gcaaccggga gcgctttaag ctgggagtga tgttccacta tgggaaactg aatatggcgt
      361 tctaagcctt cccaccatga tcggggaaga cccagatatg aacaggcaca ttcagagtta
      421 ctttaaagag cttactgaac gtctgaggtg ttagagccat gactgccgca gcattgctgg
      481 cggaaatgcc tgaatcgagc agcctctcga gacgtgaaat cagcgcactt gtcggtgttg
      541 ctcaggtaaa ccgggactcg ggaactctgc gcggtcgacg aaccattttc ggtgattgtg
      601 ctggggaaga acagctctgt acatggcggc gcttgaggca ccccggttta atctggtgat
      661 aaaggcgttt tacatgcgcc tacttgcagc cggaaacgcc aaaaaagtgg cactggttgc
      721 ctgtatgcgt aagttgctga ccattatgaa cgcaatgctc agaaaaaacg aagaatggaa
      781 tgaatcgtat ttatgaaaac tggattgtgg gccataaata ttccaaaaag atagcccgct
      841 ccgtttatta ccggaacggg cgagagcatg acattgtctg taattataat gagactgctg
      901 attctactta tttttaattt caagaaaagc gttttctaca aagaattatg agatttaatg
      961 ttttgtttag acaatattat cattctgttc aataatgaaa gtcttcatca acaacaagta
     1021 aaaacaacgt ccctctcatc gaaccggact tgcagatttc ccgcatccgg ctctcttgca
     1081 acacatcaga ccttcacgtt cgggcggtta tggggaaggt ccagcaagct gtatgagccc
     1141 taactgtttg aataccagta gattaccaga aacttctatc gattgaagac attaccaact
     1201 agcgttacta aatgttgacc aagttttgtt cacttgtctg gttttgatag tag
//
LOCUS       DAZPAC010000477         1245 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZPAC010000477 DAZPAC010000000
VERSION     DAZPAC010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.489"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..787
                     /locus_tag="ACHUPM_004748"
     CDS             <1..787
                     /locus_tag="ACHUPM_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0872270.1"
                     /translation="IAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYV
                     NQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQL
                     SDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQ
                     GNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQG
                     SRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            800..1245
                     /locus_tag="ACHUPM_004749"
                     /pseudo
     CDS             800..1245
                     /locus_tag="ACHUPM_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT          400 a          249 c          236 g          360 t
ORIGIN      
        1 tattgccaaa acacgtcacc aaattaactt atcgaattcg acctcatttt ccaaagatgg
       61 ctattcctcc aacaatactg gaattactgg catagccggt gaacatgatc agttaaatta
      121 tggtatatat gttaatcagc aacaacaaaa taatgatacc tcgcttggta cgaatttaag
      181 ctggagaact cccatcgcca taatagatgg cagctatagt cattctaaaa acgcctggca
      241 aagtggtgga agtattagta gtggattagt tgtctggccc ggtggtatta atatcactaa
      301 ccagttatcc gatacatttg caattctgga tgcgcctggg ttagaaggtg cacatattaa
      361 tggacaaaaa tacaatcgaa caaacagcaa aggccaggtt gtttacgacc tgataatacc
      421 tcatcgtgaa aaccatctgg tacttgatat agcaaacagt gaaagtgaaa cagaattgca
      481 gggcaatcgt caaattattg caccttaccg tggagcagtt tcttatgtgc agtttacaac
      541 tgaccaacgt aagccctggt atatacaggc actgcgtccc gatggttcgc cattaacctt
      601 tggctatgac gtactggatc tccaggaaaa taatattgga gtcgttggcc agggtagtcg
      661 cctttttatt cgcgtagatg aaattcccac tggaataaaa gttgctctca atgatgaaca
      721 gaacttattc tgtactatta cttttcaaca cgttatcgat gaaaacaaaa catatatatg
      781 ccagtaaaag gagtttacta tgaagtggct tttattaatc accttgagtc tatattcttt
      841 tattgtgcaa agtgctccct gtgcattgac caatgtcggg gaacaacgtg gaacatatat
      901 actcaagcca ctgagtatga aaggtaatct caccgccgaa aaaattgaaa acaccggaaa
      961 tatcattgag tgtgcagact tacaagagac gaccaacgcc attaaagttc attttttaac
     1021 acagaacgct ttaaggttta aagtcggaag tcgtcattat attattaatt tcccctttga
     1081 atctgacggt aaaaaaaata gagcttccag gaaggtcatc ttataattta gaagaaatgc
     1141 tatctgcgat aaatttcacc attccatact ctatcgcatc tgtatcagat agttccaaca
     1201 ccgcagatat ttttccagga actccttttc ctttgttgtt cgaaa
//
LOCUS       DAZPAC010000478         1228 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZPAC010000478 DAZPAC010000000
VERSION     DAZPAC010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.490"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /gene="ipaH4.5"
                     /locus_tag="ACHUPM_004750"
     CDS             complement(<1..784)
                     /gene="ipaH4.5"
                     /locus_tag="ACHUPM_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFR0872271.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTR"
BASE COUNT          349 a          222 c          267 g          390 t
ORIGIN      
        1 cgcgagtcga caatggattc cctgaaatgt taacattcgt acaaatgggc aatgtgatta
       61 tattttcggg taatgaagtt agtaggttat tggaaacatc cagtgccaat agttcttgcg
      121 gtaatctgga taatctgcgt agttgatttt cacctacctt caaatatttt aagcgctgag
      181 gaaattctgg tagttcctgt agtctgttgc cggaaacaaa gagttcttgt agtctatgag
      241 gcaattcagg caagacagta aggcgattac cttcaatacg taatattgtt agcgttgatg
      301 gaagctctgg taacgtctcc agactattaa aacgtgccga gagtgattgt aaatatggtg
      361 gtaatgatgg cagacatgac aaatttcgat tgaatgaaat attaagctta atcagttgag
      421 atggaagtgt cgggagtata ttcaagttgt taccatttac atgaagttct gtcaaaagcg
      481 gagaattttc aggtagggag attaactcat tgttggaaat attaagttca cgtatatgca
      541 aagggagtgg tggtaaagaa cgtagtttca gtaagcttaa attgagaaca ggctcccggg
      601 tatccagaca tattttcaat aatcgaaccg cctggatgcg attttctgtt gttgttcctt
      661 ccctagccca gtcatcccag atgcggtggt aatctctggt acactgttct tctaccgata
      721 aacgagatat acatgataag ccacaaaggg aacgaaaaaa agaatgattg ttgatcggtt
      781 tcatttatta acccctcact attcatatct tggtctatta atgcaaataa aacgagaaag
      841 tctttaaaaa aacaatccta gagctttatc ggcgttttca ttccagctgg aggaatatgt
      901 tcctgtcttt ccagtaaccg tcgattgtta tatcagtcta cccatgttaa tgtgaccagt
      961 tctactttca tccagtttgg attgatcttt atcccctata ttttcaaaca atttttatca
     1021 tttaactcat tactgtctcg atattcccat agcaaacgat ctggttggtt agcccctcaa
     1081 aaaagtcagt tgcacgccgt aaatccctgc cggtattgag cgaggattta cgggttgttg
     1141 ttaaggtccg taatcgggtc acagtttttc cggagtcaat ccggtctgcg gtttatgctt
     1201 atgcgacgtg attatgaatg gtgcagtc
//
LOCUS       DAZPAC010000479         1219 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZPAC010000479 DAZPAC010000000
VERSION     DAZPAC010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.491"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            43..783
                     /gene="phoN2"
                     /locus_tag="ACHUPM_004751"
     CDS             43..783
                     /gene="phoN2"
                     /locus_tag="ACHUPM_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFR0872272.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            856..978
                     /locus_tag="ACHUPM_004752"
     CDS             856..978
                     /locus_tag="ACHUPM_004752"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872273.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(1136..>1219)
                     /locus_tag="ACHUPM_004753"
     CDS             complement(1136..>1219)
                     /locus_tag="ACHUPM_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HFR0872274.1"
                     /translation="NAMKSKDSKMIDFLLKNGAVSGKRFER"
BASE COUNT          366 a          265 c          252 g          336 t
ORIGIN      
        1 gctgaccaaa atcatcaatt aattatgcta aggaaataaa ttatgaaaac caaaaacttt
       61 cttctttttt gtattgctac aaatatgatt tttatcccct cagcaaatgc tctgaaggca
      121 gaaggttttc tcactcaaca aacttcacca gacagtttgt caatacttcc gccgcctccg
      181 gcagaggatt cagtagtatt tcaggctgac aaagctcatt atgaattcgg ccgctcgctc
      241 cgggatgcta atcgtgtacg tctcgctagc gaagatgcat actacgagaa ttttggtctt
      301 gcattttcag atgcttatgg catggatatt tcaagggaaa ataccccaat cttatatcag
      361 ttgttaacac aagtactaca ggatagccat gattacgccg tgcgtaacgc caaagaatat
      421 tataaaagag ttcgtccatt cgttatttat aaagacgcaa cctgtacacc tgataaagat
      481 gagaacatgg ctatcactgg ctcttatccc tctggtcatg catcctttgg ttgggcagta
      541 gcactgatac ttgcggagat taatcctcaa cgtaaagcgg aaatacttcg acgtggatat
      601 gagtttggag aaagtcgggt catctgcggt gcgcattggc aaagcgatgt agaggctggg
      661 cgtttaatgg gagcatcggt tgttgcagta cttcataata cacctgaatt taccaaaagc
      721 cttagcgaag ccaaaaaaga gtttgaagaa ttaaatactc ctaccaatga actgacccca
      781 taaagctgga cagcctgtat caggctatgg agggcccata gacaaatcta ccctatatga
      841 gcataggagg agtctatggg cacaccacgt tttacccctg aatttaaggg attactggaa
      901 aggctgggac atatccctcc ggcagaagca gaaaaagctt attatgctgc catcggaaac
      961 gatgatctgg caacctgagt tcacagataa aacattctct aggaaactcg gggcggttcc
     1021 gttcaccaca tgcaatgtgg tgttgcaggg gaacggtctg caccatcccc tatgtcgatc
     1081 aatataacag aaatgacaac ttcagattca gggcacaacc taaatatatt ttaggtcacc
     1141 tctcaaatcg tttgcctgat accgctccat tttttaataa aaaatcaatc atttttgaat
     1201 ctttgctttt cattgcatt
//
LOCUS       DAZPAC010000480         1216 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZPAC010000480 DAZPAC010000000
VERSION     DAZPAC010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.492"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..207
                     /locus_tag="ACHUPM_004754"
     CDS             <1..207
                     /locus_tag="ACHUPM_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR0872275.1"
                     /translation="SKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKKLPLSSL
                     ELSYSERIGDSKIWNMVKGYSSFV"
     gene            217..579
                     /gene="ipgE"
                     /locus_tag="ACHUPM_004755"
     CDS             217..579
                     /gene="ipgE"
                     /locus_tag="ACHUPM_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFR0872276.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            579..1037
                     /gene="ipgF"
                     /locus_tag="ACHUPM_004756"
     CDS             579..1037
                     /gene="ipgF"
                     /locus_tag="ACHUPM_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFR0872277.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            1046..>1216
                     /locus_tag="ACHUPM_004757"
     CDS             1046..>1216
                     /locus_tag="ACHUPM_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFR0872278.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIP"
BASE COUNT          417 a          155 c          275 g          369 t
ORIGIN      
        1 agtaaattat cctcagaaga gaaaagatta ttttctacta ttctaatgaa tagtggtaat
       61 atggaaatcc aagagatgaa tactggtgtg cccggaaata aagtcatgaa aaaattgccg
      121 ctatcttcgt tagagctatc ttattctgaa agaatagggg actcaaaaat atggaatatg
      181 gtgaaagggt attcgtcatt tgtataagag gaatatatgg aagatttagc agatgttatt
      241 tgccgagcct tgggtatccc tttaattgat atcgatgatc aagcaatcat gcttgatgat
      301 gatgtgctaa tatatattga aaaagaagga gattcaataa atttattatg ccctttttgt
      361 gcattgcctg aaaatataaa tgatcttata tatgcgttga gcctaaatta ctcagaaaag
      421 atatgcttag ctactgatga tgaaggcgga aatctaattg cgcgattaga tcttaccggg
      481 ataaatgaat tcgaggatgt atatgttaat acagaatatt atatttcgcg tgtgcgttgg
      541 ctgaaggatg aatttgcgcg aagaatgaag gggtattaat gtcccgtttc gtatttattt
      601 tgctgtgttt tatcccatat ttagggagag ctgattgttg ggataaggct ggtgaaaggt
      661 acaatattcc atcaagtttg ttaaaagcga ttgcggaaaa agaatccgga tttaataaat
      721 ttgcagtcaa tgttaataac aatggaagta aagattatgg tataatgcaa ataaatgact
      781 ttcattctaa aagacttaga gaaatgggat attctgagga aatgctaatt agtcatccat
      841 gtctttctgt acattatgca gcaaagttat taaatgagtt tatgatgatg tatggaagag
      901 ggtgggaggc tgttggagcc tataatgcag gaacgtctcc gaaaaagaaa aaagaaagat
      961 taaagtacgc agaggatatc tatagacgct atttgcggat agcagcagag agcaagcaga
     1021 ataatcgaag gatataagag gatttatgtc tgaggcaaag aactcaaatc ttgcaccatt
     1081 caggttatta gtgaaactca caaacggtgt cggggatgag tttccgttat attatgggaa
     1141 taacttaatt gttctcggac ggacgataga aacacttgag tttggaaacg acaatttccc
     1201 agaaaatatt attcct
//
LOCUS       DAZPAC010000481         1215 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZPAC010000481 DAZPAC010000000
VERSION     DAZPAC010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.493"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>1215
                     /gene="traD"
                     /locus_tag="ACHUPM_004758"
     CDS             <1..>1215
                     /gene="traD"
                     /locus_tag="ACHUPM_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFR0872279.1"
                     /translation="TVGAGKSEVIRRLANYARQRGDMVVIYDRSGEFVKSYYDPSIDK
                     ILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQGSGRTIFAEAAYLMR
                     NDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIEKTAISIRAVLTNYVKAIR
                     YLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLL
                     AMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKA
                     AATLFDVMNTRAFFRSPSHKIAEFAVGEIGEKEHLKASEQYSYGADPVRDGVSTGKDM
                     ERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQERPKVAPEFIPRDINPEMENR
                     LSAVLAAREAEGR"
BASE COUNT          280 a          310 c          339 g          286 t
ORIGIN      
        1 cacggtcggg gccggtaagt cggaggttat ccgtcgtctg gccaactacg cccgtcagcg
       61 tggtgatatg gtggtgattt atgaccgttc aggggaattt gttaaaagtt actatgatcc
      121 ctccatcgat aagatcctga atccgctgga tgcacgctgt gccgcctggg atttgtggaa
      181 ggagtgcctg acacagccgg attttgataa taccgcaaat actctgatcc cgatgggcac
      241 aaaagaggac ccgttctggc agggttcagg acgtaccatt tttgcggaag cggcgtatct
      301 gatgcgtaat gaccccaacc gcagctacag caaactggtg gacacactgc tttccatcaa
      361 aattgaaaaa ctgcgtacct acctgcgtaa ttcaccggcg gccaacctgg tggaagagaa
      421 aattgagaaa acggcgattt ccatccgtgc tgtgctgacc aactatgtga aagccatccg
      481 ttacctgcag gggattgagc ataacggtga gcccttcacc atccgtgact ggatgcgtgg
      541 tgtccgggag gatcagaaaa acggctggct gtttatctcg tcgaatgccg acactcatgc
      601 ctccctgaag ccggtgatct ccatgtggct gtccattgcc attcgtggtc tgctggcaat
      661 gggagaaaac cgtaatcgtc gtgtgtggtt cttctgtgat gagttaccca cattacacaa
      721 actgccggac ctggtggaga tcctgccgga agcccgtaag ttcggtggct gttatgtgtt
      781 tggtatccag tcctatgccc agctggaaga tatctacggt gagaaagcgg ctgccacgct
      841 gtttgacgtc atgaacaccc gtgccttttt ccgttctccc agccataaga ttgcggagtt
      901 cgctgtcggt gaaattggtg agaaagagca cctgaaagcc agcgaacagt attcctacgg
      961 tgctgatccg gtacgtgacg gggtatcgac cggtaaggat atggagcgcc agacgctggt
     1021 cagttattcc gacattcagt ccctgccgga tctgacctgc tatgtcaccc tgcccggacc
     1081 gtatccggca gtcaaactct cactgaaata tcaggaacgt ccgaaggtcg ctccggagtt
     1141 tattccgcgt gacatcaacc cggaaatgga gaaccgtctg agtgccgtac ttgccgcaag
     1201 ggaagcagaa ggtcg
//
LOCUS       DAZPAC010000482         1212 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZPAC010000482 DAZPAC010000000
VERSION     DAZPAC010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.495"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            230..>1212
                     /gene="gspA"
                     /locus_tag="ACHUPM_004759"
     CDS             230..>1212
                     /gene="gspA"
                     /locus_tag="ACHUPM_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR0872280.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQDIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSG"
BASE COUNT          328 a          249 c          310 g          325 t
ORIGIN      
        1 gcatctttat tatgattcgc cagatggaag gggaaacgta gtgtgggcac gatgtatgtt
       61 ctaataaaat agattgggtg ggttgtatat tgacgttagt ataaatagta ttaatgccgc
      121 ttgcatgaat caagcaaaat ttatttatat tagtaatatg tatttaatta ataaatacat
      181 acattatttt tatgattaag gataatcaat aattaaaaca ggaagttcta tgtctacgag
      241 aagagaagtt attctttcct ggttgcgtga gaaacgtcag acctggcgtc tgtgctattt
      301 gttgggtgag gctggaagtg gaaaaacctg gctggcgcag caactgcaaa aagataaaca
      361 tcgccgtgtg attactttaa gcctcgttgt ttcctggcaa ggtaaggccg catggatcgt
      421 taccgacgat aacgcggctg aacagggctg ccgtgacagc gcctggacgc gagatgagat
      481 ggcggggcaa ttactgcatg cgcttcatcg aactgatagt cgctgccctc ttattattat
      541 tgaaaatgct cacctgaatc accgccgcat acttgatgat ctccaacgcg cgatatctct
      601 tattcctgac ggtcaatttt tgctgatcgg cagacccgat cgaaaagtcg aacgtgattt
      661 taaaaaacag gacattgaac ttgtctcgac aggacgtctg acggagcacg aacttaaggc
      721 aagcatcctt gaaggtcaga atattgacca acccgatctc cttttaacct ccagagttct
      781 gaaacggata gctttattat gtcggggcga tcgcagaaag ctggcgcttg ccggcgaaac
      841 aattcgctta ttacagcaag ccgagcagac cagcgttttc actgcaaaac aatggcgaat
      901 gatttaccgc atattgggtg ataaccgccc ccggaaaatg cagcttgccg tggtaatgag
      961 tggaaccatt atcgctatta cctgtggttg gctactgctg tcctctttca cagctccgtt
     1021 ccccgttcct gcgtggctgg tacccgtaac gccagtagtt aaacaagata tgactaagga
     1081 tattgctcat gtggtgatgc gcgatagtga ggcgctaagc gtgttatacg gcgtatgggg
     1141 gtacgaggtc ccagctgatt cggcctggtg cgatcaggca gtaagagcgg ggctggcatg
     1201 taaaagtggg aa
//
LOCUS       DAZPAC010000483         1211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZPAC010000483 DAZPAC010000000
VERSION     DAZPAC010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.496"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..680)
                     /locus_tag="ACHUPM_004760"
     CDS             complement(<1..680)
                     /locus_tag="ACHUPM_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0872281.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
     gene            complement(996..>1211)
                     /locus_tag="ACHUPM_004761"
     CDS             complement(996..>1211)
                     /locus_tag="ACHUPM_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR0872282.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          355 a          211 c          252 g          393 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata ttctgataaa atgtgatact
      721 gggaaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagagatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcatat ttgaacgttc
      901 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccgta
      961 gcacgtctta agcaagaacg tgctgcggtt ggatgctatt ttttccctga agcggaaaac
     1021 attactacag taccttgaac cttggtttta acattctcga aatgctctga gagtatatgt
     1081 gttaagcctt cttcggaatc ttttgtgttt gaaaagatgc ctttctgatt gtaaatgcgc
     1141 atcagttttt gaccgaagct attgtgcaca actccatcgc caagaattgt ggctccgtat
     1201 agagttccat c
//
LOCUS       DAZPAC010000484         1211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZPAC010000484 DAZPAC010000000
VERSION     DAZPAC010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.497"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            54..1211
                     /locus_tag="ACHUPM_004762"
                     /pseudo
     CDS             54..1211
                     /locus_tag="ACHUPM_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT          321 a          305 c          273 g          312 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
      841 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
      901 tttacgccgg acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt
      961 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
     1021 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
     1081 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
     1141 aagttttact caataacgat tactggtgtc gggcattatc atttagtcct acgaaagcga
     1201 agaactatca g
//
LOCUS       DAZPAC010000485         1209 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZPAC010000485 DAZPAC010000000
VERSION     DAZPAC010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.498"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..413
                     /gene="espX1"
                     /locus_tag="ACHUPM_004763"
     CDS             <1..413
                     /gene="espX1"
                     /locus_tag="ACHUPM_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFR0872283.1"
                     /translation="SDLSNTIFSNGEINNLFIKKSNIFGTSFTNTMIKNLRCEDIMPG
                     RWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELN
                     KFMHDYDKTSSKMRKYLQSINECMLLIKNIVHQD"
     gene            647..1209
                     /locus_tag="ACHUPM_004764"
                     /pseudo
     CDS             647..1209
                     /locus_tag="ACHUPM_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          437 a          225 c          203 g          344 t
ORIGIN      
        1 aaagcgactt atcaaacacc atcttttcaa atggagaaat aaacaacctc ttcattaaaa
       61 aaagcaacat atttggaaca tcgttcacta ataccatgat aaaaaatttg cgctgtgaag
      121 atattatgcc tggccgatgg accactcaac ttgtcaataa gcacttaggt tacagataca
      181 caggagtatt caaaaccctg gcaagtattg atgacaaacc gtcccgtttc gaaattttga
      241 taccattggt acaaacttta gttcgcgata atgtaaagct aaataatgat gtgtataaag
      301 aacttaataa gtttatgcac gattacgaca aaacatcatc aaaaatgaga aaataccttc
      361 agagcatcaa cgagtgcatg ctccttataa aaaatatagt tcatcaggat taaaacactc
      421 aaagaaagag gtaattttag aatgaaatta tctgtgatga ttattaatac actgcagaga
      481 aataaaaaac aatctgaagc atgctctgaa acatcctact ctgtcactct aatgatggca
      541 ccttgctctt cttataaaga agggaacaat taagaatatt aaattttcaa actttcatca
      601 acttcacatc aagaaattaa aaaatttaaa cattaatcta aataataaca acctgatcac
      661 ataatcgggt acgattttat ccgataacta taatgctata actctgggaa atggttggaa
      721 tacaccgctg ggtgccatct ctttttacgc tgcccgatcg agtagtaagt taaataatga
      781 taccagacac gaaggtacga gctatcaggt cgcctataat aaatatctag tacaaacagc
      841 cactcgattt agcgtggccg cctggcgtta tgcttcgcag gattacagga cattcagcga
      901 ccatctttac gaaaatgata aacactatca tcagagtgat tatgatgatt tttatgatat
      961 tggcagaaaa aatagccttt ctgccaatat tatgcaacct ttatccaata atctgggaaa
     1021 tgtatcatta agtgcgcttt ggcggaatta ctgggggcga agtggaaatg ctaaagatta
     1081 ccaattcagt tactccaata actggcaaca cattagttat actttctctg ccagccaatc
     1141 ttatgatgaa aataataaag aagaggagcg ttttaatctg tttatctcca ttcctttcta
     1201 ctgggggga
//
LOCUS       DAZPAC010000486         1206 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZPAC010000486 DAZPAC010000000
VERSION     DAZPAC010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.499"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..729)
                     /gene="efeU"
                     /locus_tag="ACHUPM_004765"
     CDS             complement(<1..729)
                     /gene="efeU"
                     /locus_tag="ACHUPM_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR0872284.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAP"
BASE COUNT          354 a          330 c          280 g          242 t
ORIGIN      
        1 cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg agtgagttga
       61 gagcactgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc cggcttcatg
      121 aaatgcgcga atggcaccag cagccagccc tgcggcgacg aagagaataa acaggctggt
      181 ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca ggaagcctag
      241 caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc cgacatcttg
      301 ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa cggcaaaaaa
      361 gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc tatcgactgc
      421 ctgctccagt tgtactttga cgttgcgcga cactttgcgc atccagaaaa ccatccaggt
      481 aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgtt ctttttgcgg
      541 aaattcgccg gtggtttcgt taatgaagat acccaagccc aggcacaacg cagcggcaag
      601 caacacgcca atccacatca cgccaatcca tcggcctcgc tgggtacgct taagatagct
      661 ggcaatcaaa ctgacaatca gcgcggcttc aagtccttcg cgcaacataa tgagaaacgg
      721 aacaaacatg ccacccaccc ttaaacgttt ttctgagtca atccatgcaa agaaaagtaa
      781 atcgcagtga tagcgattat cattacggca aagaaaaata caagcggaat gtttataaaa
      841 tgatgttgga atgtaaacaa agagaggtaa taccagggga tataaacgat tattccaggt
      901 acaaagatgg gattgtttac aacgagccat gtgttcttgc cagatgccgc gtaaacgcct
      961 tatccagccg acaaaactgt ccagactcaa tgcatcacag cttccatgta aaactgataa
     1021 gcgggtgtat caagcaattt cgtccatccg cgattatcaa atctaacttt ttgcccggaa
     1081 cataataaca gcgcgggtgt tggtggcgtc aaaatcgctg aaacgttccc cgaaggccgg
     1141 ggcagcccac atggatgtgg gctgagggcg cgtcttacag gacgttacct cgcgcccgac
     1201 ccggta
//
LOCUS       DAZPAC010000487         1198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZPAC010000487 DAZPAC010000000
VERSION     DAZPAC010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.500"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            223..1038
                     /gene="yahL"
                     /locus_tag="ACHUPM_004766"
     CDS             223..1038
                     /gene="yahL"
                     /locus_tag="ACHUPM_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFR0872285.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          364 a          230 c          233 g          371 t
ORIGIN      
        1 tggtttaaca tattaacttc gctctccact taacttttta gttaaggaga gcgaaataat
       61 atcaaagtag cagtaaaacc tataacgtaa attcaaattg ttaaattaac tccctccagt
      121 acacaatact tctcacgtta gttatgagcg atttctgata gtgcctggtt taatcagcgc
      181 tttattatct gcgacgttta tttttattta agagagatcg cgatgatatc attaaaagct
      241 ccgcacaata atttgatgcc atatacgcaa caaagcatac ttaatacggt taaaaacaat
      301 cagttaccag aggatatcaa aagctcccta gtttcctgtg tcgatatatt caaggttttg
      361 attaaacaat attatgatta tccttatgat tgtcgtgatg atttagtcga tgacgacaaa
      421 ctcattcatc tcatggctgc cgtacgagac tgtgagtggt cagacgataa cgcactcacc
      481 ataaatgtgc agtttaatga tttcccagga ttttatgact ggatggatta ccctgatcac
      541 ccggttaagt ttgtttttcg catacttgaa aatcaaaaag ggacagtttg ggtatatgac
      601 caggatgatg cgtttcttga tattaaagca aatgtgcagg ctggacgctt taccgggctt
      661 aagaaattag tgcagtttat caattcagtg cgaacagatt gcaaatgtat tcttcttgaa
      721 caccatatgc ctttgctgag acttttccct aaagggaaag agtgtatgca tgtggaaaaa
      781 tggcttcgcg aaatgtcgtc tattccggag acagacgcgc ccattaagca ggcgcttgcg
      841 catggattat tattacactt aaaaaatatc taccctgttt ttcctgaatc actggtgatg
      901 ttattgctct ctgttctgga cgtaaaaaca tacagagatg atgcacgatt aaatgaatgg
      961 atatctaata gagtgcagga attaggtgac agatattatc ctgtaaataa gcacgttaaa
     1021 atccgctaca ctctttagtt cagaaagggt tccacctgca agttgttatt ccaccagaga
     1081 gtgaatcgca aagcaggtaa tcatttcatc tgtatatccg tatgccaaat ggcattccac
     1141 cgccttttcg ccgatatttt gttgtgatcg gcgacacttc gcttaaaaaa gcaccagc
//
LOCUS       DAZPAC010000488         1192 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZPAC010000488 DAZPAC010000000
VERSION     DAZPAC010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.501"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            5..1072
                     /gene="mxiC"
                     /locus_tag="ACHUPM_004767"
     CDS             5..1072
                     /gene="mxiC"
                     /locus_tag="ACHUPM_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR0872286.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            1085..>1192
                     /locus_tag="ACHUPM_004768"
     CDS             1085..>1192
                     /locus_tag="ACHUPM_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872287.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYL"
BASE COUNT          414 a          163 c          236 g          379 t
ORIGIN      
        1 atgtatgctt gatgttaaaa atacaggagt ttttagctct gcattcattg ataagttgaa
       61 tgcaatgaca aattcagatg atggagatga gactgctgat gcagagcttg attctggctt
      121 ggctaatagc aagtatattg actcatctga tgagatggct tccgctcttt cgtcatttat
      181 aaacagaaga gaccttgaga aactgaaagg aacaaatagt gatagtcagg aacgtatttt
      241 agatggggaa gaagatgaaa ttaatcacaa gatttttgat ttaaagagaa cgttaaaaga
      301 taaccttcct ctagatcggg attttataga cagactaaag agatatttta aagatccaag
      361 tgatcaagtc ttagcattaa gggaactttt gaatgaaaaa gatcttactg ctgaacaagt
      421 cgaatcatta actaaaatta ttaatgagat aatatcaggt agtgaaaaaa gtgttaatgc
      481 tggaataaat tcagctatac aggctaaatt atttggcaac aaaatgaaac ttgaaccaca
      541 gcttttgcgt gcatgttatc gtggttttat catggggaac atatcaacaa cagatcagta
      601 tatagaatgg cttggtaatt ttggttttaa tcacagacat acaattgtga attttgtaga
      661 gcagtcactg attgtagaca tggattctga gaaaccgagc tgtaatgctt atgagtttgg
      721 ttttgtgtta tctaaattaa ttgcaattaa gatgattaga acttcagacg taatttttat
      781 gaagaaactg gaatcctcaa gcttgctaaa agatggcagt ttaagtgcag agcagctatt
      841 gctaacttta ttatatattt ttcaatatcc aagtgaaagt gagcaaattc ttacttctgt
      901 tatagaagta tcacgagcca gtcatgagga ttctgtagtg tatcaaacat atctatcttc
      961 tgttaatgaa agtcctcatg atatatttaa aagtgaaagt gaaagagaaa ttgcgatcaa
     1021 tattctacga gagcttgtca caagtgcata caagaaagag ctttctagat aacaggagat
     1081 gaaagtgatc cagtcttttc ttaagcaagt aagtactaag cctgaattaa ttatattagt
     1141 gcttatggtt atgatcattg ctatgttgat cataccatta cctacatacc tt
//
LOCUS       DAZPAC010000489         1179 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZPAC010000489 DAZPAC010000000
VERSION     DAZPAC010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.502"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1000
                     /gene="htrE"
                     /locus_tag="ACHUPM_004769"
     CDS             <1..1000
                     /gene="htrE"
                     /locus_tag="ACHUPM_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFR0872288.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIILNGIRCQIQ"
     gene            1017..1121
                     /locus_tag="ACHUPM_004770"
                     /pseudo
     CDS             1017..1121
                     /locus_tag="ACHUPM_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          356 a          236 c          276 g          311 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 atctattatt ctgaatggaa ttcggtgtca gattcagtaa ctacaaggaa cgtcaaatga
     1021 taaaaataac gccacataaa atagcgatac tgatgggaat attattatca ccatcagtat
     1081 ttgcaacgga tattaatgta gagtttacag ccactgtcaa agtagactgg ccccctgaat
     1141 ctccagacaa ccagtatcac ttaaataagt gatagtctt
//
LOCUS       DAZPAC010000490         1171 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZPAC010000490 DAZPAC010000000
VERSION     DAZPAC010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.503"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            75..860
                     /locus_tag="ACHUPM_004771"
     CDS             75..860
                     /locus_tag="ACHUPM_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872289.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(997..>1171)
                     /gene="ykfM"
                     /locus_tag="ACHUPM_004772"
     CDS             complement(997..>1171)
                     /gene="ykfM"
                     /locus_tag="ACHUPM_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HFR0872290.1"
                     /translation="IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCN
                     NEYISRKEDSAVK"
BASE COUNT          386 a          207 c          250 g          328 t
ORIGIN      
        1 tcaagtggag tatataatta aaaatcgtta gctaaacatt accatgatta tgacaggcga
       61 gggagtgtcc gattatgaat ttaagaaggg tttgttgtat atgtgtatta ttctcgctgc
      121 ttgcaggatg tgtctctgaa tcttctattg atgaaaagaa gaaaaaagct caagtcacac
      181 aaagtaatat taataaaaac actccccagc aactgacaga caaagattta ttcggtaatg
      241 aaaccactct ggccgtatcc gaagaggata ttcaagctgc gcttgatgga gatgagttcc
      301 gcgttcccct taattctcct gtaatacttg ttcaatccgg caaccgcgca ccggaaacca
      361 ttatgcagga ggagatgcga aaatattata ctgtttccac attctctggt atcccggaca
      421 ggcaaaagcc tctgacttgt aacaaaaaca aagataaaaa tgagaacgag gatgttgcca
      481 gtgctgagaa tatgaactgg atgcaggcac tgcgttttgt ggctgcaaaa gggcatcaga
      541 aagcgattat tgttttccag gatacgttgc agacaggaaa atatgactct ggattgaaat
      601 caatagtttg gtcagattat aaaaatcaaa aattgactga cacgatgtca ctgcgctatc
      661 tggtacgatt cacgctggtg gatgtagcta caggtgagtg ggctgcctgg tcgccggtga
      721 attatgaata taaagtgctt ccaccattgc ccgacaagaa tgaagccagt acgactgata
      781 tgacagagca gcaaatcatg caacttaagc aaaagaccta taaagcgatg gtaaaagatt
      841 tggttaaccg ctatcaataa taaattatat ctgccgccag gaatttctgg cggaaataat
      901 acaaaattct ttgcataaga aatttttctt gacaaagaca gtgcaagaaa ggacatggaa
      961 tccggagtta aaaactgttt gttttagaat gtttaattat ttaactgctg aatcttcctt
     1021 gcgagaaata tattcattat tacatttcat gccattttta atatagattg ctcctgggaa
     1081 aattcccgta ttattaccgc aggggtgata attagcattg acatatcctg tgacaaaagg
     1141 agctattaaa ggtgctatta cgatagctat t
//
LOCUS       DAZPAC010000491         1161 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZPAC010000491 DAZPAC010000000
VERSION     DAZPAC010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.504"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            247..492
                     /gene="yahM"
                     /locus_tag="ACHUPM_004773"
     CDS             247..492
                     /gene="yahM"
                     /locus_tag="ACHUPM_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFR0872291.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(509..>1161)
                     /locus_tag="ACHUPM_004774"
     CDS             complement(509..>1161)
                     /locus_tag="ACHUPM_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR0872292.1"
                     /translation="FMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRR
                     AGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQS
                     TPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWA
                     GIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          352 a          277 c          275 g          257 t
ORIGIN      
        1 tttcgcagcc atgcggtgta taaaaaatga tctcatgcag atgttttgtg aatgtgtggg
       61 ttgacattca tatgaaaaaa atcataattc catcatgttt gtgtggggtc ttttctgtac
      121 cttacgcatc gcactcaagc ctgacagaaa atatgctgta aggctcatat caaaaaccgc
      181 cattagctca tcaggaagag cagacgacaa ccaggattgt tgtatggtac gggggtcgag
      241 gcctcgatgg cggtccaact tttcaaaact cttctgaatc agatcccctt attatcatcc
      301 ctgcaaagtg gaacattgcc attgtttggc tattcaggac gggtcagacc aatgaaaaaa
      361 gcccacacag gggagagtgg gctgaaatgg gaagctaaag actcaagtaa acttatcgga
      421 aataaggacc acgcattacg ggggctatca tcgccgatag tcgggattcg gcaaatcagg
      481 ttgattacgt agagagtaaa ttatctgctc accgctgcgt caccccttcg taaatcaggc
      541 gtagcgcgaa tacaccaata attgcaccaa taacccgact ggcaacgcgt tgcatacgcc
      601 cataagcacg acgcacagcg ggcaaagaaa acgcctgact aagaaagact cgccagataa
      661 ctgatgcgag cacaatcccc gcccaggcca ttaaacgtgc ccatgttggt gtttcggcat
      721 ttaatgttac tgagaaaata ctgataaaaa ataaaacggt ttgcgggtta gagagatcgg
      781 taattaatcc gcggcgaaaa aagacatacc aggaggcgct aatcggttgt tgtagtgtgc
      841 tcatttgcgg tgttgactgg cggcgcatgc tgcaccacgc aaaccataag agataagcgc
      901 cgccgacgat tctgataagc gaaaaaatct cctcacactg cgttattagc gttgcaagac
      961 caaacaaacc caacccggaa taaaatgcat cgcccagcgc cacgcccagt ccggtcagca
     1021 cccctgcgcg tcgaccggaa gccaggctgg tttgtactac cacaaagaga ttggctcccg
     1081 gattaaaaaa agtaatcacg aacagtccta cggtcaggta aacggcgtgc aaaggatcca
     1141 tagtgatttc atccataaat a
//
LOCUS       DAZPAC010000492         1157 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZPAC010000492 DAZPAC010000000
VERSION     DAZPAC010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.505"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..106
                     /locus_tag="ACHUPM_004775"
     CDS             <1..106
                     /locus_tag="ACHUPM_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0872293.1"
                     /translation="TNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            117..554
                     /locus_tag="ACHUPM_004776"
                     /pseudo
     CDS             117..554
                     /locus_tag="ACHUPM_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            554..931
                     /locus_tag="ACHUPM_004777"
     CDS             554..931
                     /locus_tag="ACHUPM_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872294.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          378 a          163 c          302 g          314 t
ORIGIN      
        1 aacgaatgat aaggtattca aggcagggtt aagacgtaag atgcgaaaag cagccatgga
       61 tagaaactat ctcgcgtcag tccttgcggg gagcgggctt tcgtaatctt tccctaggga
      121 gtcagccgaa tgttgggaag gatttgacgg atgaagagaa gaactgcttg gccggcggca
      181 gtacgggaag cccgggagga tggggaccag aagatgagaa tagcgcaagg gatcgtatgc
      241 agcatgaacg gtataaagat gaattgcgac agaaaatggg aaaacccgac gttacagatt
      301 caaatttgaa aaatattata gacgatcttt atcggccaaa tgcaaaagta ggaagtggaa
      361 gcacagcaga tgctgttcgt tatgaattgg ccacaggtga aaaagttggt ggtagaggac
      421 atgtattgaa agcacaggat tatacaaggg ctttacagga ttggttggat aagaatccaa
      481 cagcatctcc aggggatcga gcagcagcag aaaacgttct tcaggattta cagaatgctt
      541 tgggaggcaa gtaatgaacc aatatattgc tagttatata gaaaaaatgg catcattttc
      601 cactgttttg tctgagacag tctcattagc taatgaatac tggcatccag aaacgccacc
      661 ggtggttatg ttgttctctc aggttggaaa aacgattgta aaaaattttt atgaaatggg
      721 ggatattgaa agagaaaaaa tatttaaaca tgtcgaagat ggtatggtct cgcttgatga
      781 tgaattggcc acagttgtag ctacagggct tatagaggca atagtcactg cgaccgatag
      841 caattcttta ctttggcaag atttaagtaa atttctgggt gacaaatcaa agtctcatgc
      901 attagtgtgg aaaaattttg gtcaggagtg atattattca agaagtatta agctcgctat
      961 tgagatctta atgcttcttg agattaaatt aaagttagga cgaagtaatt gcaggtggaa
     1021 ataaatccat agtgacgtgt ggcaatggct attagatgtt atggtgtgat gggctttggt
     1081 gatttaagag ctaagttaga ttgccttatg aaaaagtatg gttatatatt taatgatgtg
     1141 tatcatatat ttaaata
//
LOCUS       DAZPAC010000493         1148 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZPAC010000493 DAZPAC010000000
VERSION     DAZPAC010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.506"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHUPM_004778"
     CDS             <1..122
                     /locus_tag="ACHUPM_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872295.1"
                     /translation="HFLPVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF"
     gene            119..376
                     /locus_tag="ACHUPM_004779"
     CDS             119..376
                     /locus_tag="ACHUPM_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872296.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="ACHUPM_004780"
     CDS             376..750
                     /locus_tag="ACHUPM_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0872297.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..>1148
                     /locus_tag="ACHUPM_004781"
     CDS             747..>1148
                     /locus_tag="ACHUPM_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0872298.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          325 a          251 c          317 g          255 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaag
//
LOCUS       DAZPAC010000494         1144 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZPAC010000494 DAZPAC010000000
VERSION     DAZPAC010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.507"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>1144
                     /locus_tag="ACHUPM_004782"
     rRNA            <1..>1144
                     /locus_tag="ACHUPM_004782"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          275 a          259 c          361 g          249 t
ORIGIN      
        1 cgaagatacc agctggctgc aactgtttat taaaaacaca gcactgtgca aacacgaaag
       61 tggacgtata cggtgtgacg cctgcccggt gccggaaggt taattgatgg ggtcagcgca
      121 agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt aactataacg gtcctaaggt
      181 agcgaaattc cttgtcgggt aagttccgac ctgcacgaat ggcgtaatga tggccaggct
      241 gtctccaccc gagactcagt gaaattgaac tcgctgtgaa gatgcagtgt acccgcggca
      301 agacggaaag accccgtgaa cctttactat agcttgacac tgaacattga gccttgatgt
      361 gtaggatagg tgggaggctt tgaagtgtgg acgccagtct gcatggagcc ggccttgaaa
      421 taccaccctt taatgtttga tgttctaacg tggacccgtg atccgggttg cggacagtgt
      481 ctggtgggta gtttgactgg ggcggtctcc tcctaaagag taacggagga gcacgaaggt
      541 tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg cataagccag cttgactgcg
      601 agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg atccggtggt tctgaatgga
      661 agggccatcg ctcaacggat aaaaggtact ccggggataa caggctgata ccgcccaaga
      721 gttcatatcg acggcggtgt ttggcacctc gatgtcggct catcacatcc tggggctgaa
      781 gtaggtccca agggtatggc tgttcgccat ttaaagtggt acgcgagctg ggtttagaac
      841 gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg agaactgagg ggggctgctc
      901 ctagtacgag aggaccggag tggacgcatc actggtgttc gggttgtcat gccaatggca
      961 ctgcccggta gctaaatgcg gaagagataa gtgctgaaag catctaagca cgaaacttgc
     1021 cccgagatga gttctccctg accctttaag ggtcctgaag gaacgttgaa gacgacgacg
     1081 ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct aaccggtact aatgaaccgt
     1141 gagg
//
LOCUS       DAZPAC010000495         1142 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZPAC010000495 DAZPAC010000000
VERSION     DAZPAC010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.508"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            6..407
                     /locus_tag="ACHUPM_004783"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACHUPM_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1142
                     /locus_tag="ACHUPM_004784"
     CDS             572..>1142
                     /locus_tag="ACHUPM_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR0872299.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLICLCY
                     SQISLAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPMADFSS
                     VSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPRLNKLV
                     TEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          319 a          255 c          271 g          297 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctaat ctgtctgtgt tactctcaga tatccctggc
      721 gggtattgtc cgttccgata ttgcctatca gatttatcgt gatttcgccg aaaacaaagg
      781 gctttttgta cctggtgcca atgatattcc ggtatatgat aaggacggaa aacttgtggg
      841 aagactgggt aaagccccaa tggccgattt cagcagtgtg agctcaaatg gcgttgctac
      901 gcttgtatcg cctcagtata tcgtcagcgt aaagcataac ggaggatatc ggagtgtgag
      961 ctttggtaat gggaaaaata catattccct tgttgaccgt aataaccacc cttctattga
     1021 cttccatgct ccacgtctga ataaactggt tacagaagtt attccctcag cggtaacatc
     1081 agaaggaacc aaagccaatg cttataaata cactgaacgt tacaccgctt tttatcgggt
     1141 gg
//
LOCUS       DAZPAC010000496         1137 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZPAC010000496 DAZPAC010000000
VERSION     DAZPAC010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.509"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>1137
                     /locus_tag="ACHUPM_004785"
     CDS             <1..>1137
                     /locus_tag="ACHUPM_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HFR0872300.1"
                     /translation="YRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVL
                     DSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMA
                     SPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLD
                     EMIVLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYI
                     ENRMYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVD
                     FDSDKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFQPLQRDMIGKFGAAKDQ
                     WFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEG"
BASE COUNT          263 a          298 c          322 g          254 t
ORIGIN      
        1 ctaccgtaac cagctggcgt ttatcgatat tttcttccgg ggaatgaata acaccaacta
       61 caacatggcg gtctgtggca cctccggggc cggtaaaacc gggctgatac agccacttat
      121 ccgcagcgtg ctggactccg ggggctttgc cgtggtgttc gacatggggg atggctacaa
      181 gtccctgtgt gagaacatgg gtggcgtata tctggacggt gaaaccctgc gttttaaccc
      241 gtttgcgaac atcaccgata tcgaccagtc agcggagcgt gtccgtgacc agttgtcagt
      301 gatggccagc cccaacggta acctggatga agtgcatgaa ggtctgctgc tgcaggcggt
      361 cagggcctcc tggctggcca aagagaacag ggcacgtatt gatgacgtgg tggatttcct
      421 gaaaaacgcc agtgacagcg agcagtatgc cgggtcaccg actatccgca gccgtctgga
      481 cgaaatgatt gtgctgcttg accagtacac tgccaacggc acttacggcc agtattttaa
      541 ctctgatgag ccgtccctgc gggatgacgc aaaaatggtg gtgctggagc tgggcggact
      601 ggaagaccgt ccgtcactgc tggttgcggt gatgttctcc ctgattatct acatcgagaa
      661 caggatgtac cgcacgccgc gtaacctcaa gaaactgaac gtcattgatg aaggctggcg
      721 tctgctggac ttcaaaaatc acaaggtcgg tgagtttatt gagaaaggtt accgtaccgc
      781 ccgtcgtcat accggtgctt atatcaccat cacacagaac atcgtcgact ttgactctga
      841 caaggcctcc agtgcggccc gtgcggcatg gggtaactcc tcctacaaaa ttatcctcaa
      901 acagagtgcg aaggagttcg caaaatacaa ccagctgtat ccggaccagt tccagccact
      961 gcagcgcgac atgattggta agtttggtgc ggccaaagac cagtggttca gttccttcct
     1021 gctgcaggtg gaaaaccatt cctcctggca ccgcctgttt gtggacccgt taagccgcgc
     1081 catgtacagc tctgacggcc cggattttga gtttgtgcag cagaaacgta aagaggg
//
LOCUS       DAZPAC010000497         1130 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZPAC010000497 DAZPAC010000000
VERSION     DAZPAC010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.510"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>1130
                     /gene="ospD3"
                     /locus_tag="ACHUPM_004786"
     CDS             <1..>1130
                     /gene="ospD3"
                     /locus_tag="ACHUPM_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR0872301.1"
                     /translation="TKNGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDE
                     KHQKCYGLISDGMSIFVDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTR
                     VVQLSDLSDNTLELLLAAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVE
                     LLTAEGMGGRISGLSQALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVH
                     RQIPGLMFALQNGHADAIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNG
                     QADAILAYGDILNEAKLNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHT
                     ADLTPHQASKLLAAEGPNGVSGLIIAFQNRNFEAIKTYMEII"
BASE COUNT          387 a          206 c          222 g          315 t
ORIGIN      
        1 actaaaaatg ggggggattt tgtcattcag ctttatgatc ccaaccatac agcaactcat
       61 ttacgagcag agtttaacaa atttaactta gctaaaataa aaaaactgac tgtagataat
      121 tttcttgatg aaaaacatca gaaatgttat ggtcttatat ccgacggtat gtctatattt
      181 gtggacagac atactccaac aagcatgtcc tccataatca gatggcctaa taatttactt
      241 caccccaaag ttatttatca cgcgatgcgt atgggattga ctgagctaat ccaaaaagta
      301 acaagagtcg tacaactatc tgacctttca gacaatacgt tagaattact tttggcagcc
      361 aaaaatgacg atggtttgtc aggattgctt ttagctttac aaaatgggca ttcagataca
      421 atcttagcat acggagaact cctggaaact tctggactta accttgataa aacggtagaa
      481 ctactaactg cggaaggaat gggaggacga atatcgggtt tatcccaagc acttcaaaat
      541 gggcatgcag aaactatcaa aacatacgga aggcttctca agaagagagc aataaatatc
      601 gaatacaata agctgaaaaa tttgctgacc gcttattatt atgatgaagt acacagacag
      661 atacccggac taatgtttgc tcttcaaaat ggacatgcag atgctatacg cgcatacggt
      721 gagctcattc ttagcccccc tctcctcaac tcagaggata ttgtaaattt gctggcctca
      781 aggagatatg acaatgttcc cggacttctg ttagcattga ataatggaca ggctgatgca
      841 atcttagctt atggtgatat cttgaatgag gcaaaactta acttggataa aaaagcagag
      901 ctgttagaag cgaaagattc taatggttta tctggattgt ttgtagcctt gcataatgga
      961 tgtgtagaaa caattattgc ttatgggaaa atacttcaca ctgcagacct tactccacat
     1021 caggcatcaa aattactggc agcagaaggc ccaaatgggg tatctggatt aattatagct
     1081 tttcaaaata ggaattttga ggcaataaaa acttatatgg aaataataaa
//
LOCUS       DAZPAC010000498         1130 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZPAC010000498 DAZPAC010000000
VERSION     DAZPAC010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.511"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1108
                     /locus_tag="ACHUPM_004787"
     CDS             join(<1..204,204..1108)
                     /locus_tag="ACHUPM_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
                     /protein_id="HFR0872302.1"
                     /translation="SEALKLAERIGVAAAARELSLYESQLYAWRSKLQQQMTSSERES
                     ELAAENARLKRQLAEQAEELAILPKGRDILCEAPEMKYVFIEKHQAEFSIKAMCRVLR
                     VARSGWYTWCQRRTGISPRQQFRQHCDSVVLAAAFTRSKQRYGAPRLTDELRAQGYHF
                     NVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYASGPNQKWAGDITY
                     LRTDEGWLYLAVVIDLWSRAVIGWSMSPRLTAQLACDALQMALWRRKRPRNVIVHSDR
                     GSQYCSADYQALLKWHNLRGSMSAKGCCYDNACVESFFHSLKVECLHGEHFISREIMR
                     ATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
BASE COUNT          257 a          294 c          339 g          240 t
ORIGIN      
        1 gcagtgaagc cctgaagctt gctgaacgca tcggtgttgc tgccgcagcc cgtgaactca
       61 gcctgtatga atctcagctc tatgcgtggc gcagcaaact gcagcaacag atgacctcgt
      121 ctgagcgtga aagtgaactg gccgctgaaa atgcccgcct gaagcggcaa ctggcggagc
      181 aggcagagga actggctatc ctccaaaagg ccgcgacata ctttgcgaag cgcctgaaat
      241 gaagtatgtc tttattgaaa aacatcaggc tgagttcagc atcaaggcaa tgtgccgcgt
      301 gctccgggtg gcccgcagcg gctggtatac gtggtgtcag cggcggacag ggataagccc
      361 tcgtcagcag ttccgtcagc actgcgacag cgttgtcctc gcggcggcct ttacccggtc
      421 aaaacagcgt tacggtgcac cacgcctgac ggatgaactg cgtgctcagg gttaccactt
      481 taacgtaaaa accgtggcgg caagcctgcg ccgtcagggg ctgagggcaa aggcctcccg
      541 gaagttcagc ccggtcagtt accgcgcaca tggcctgcct gtgtcagaaa atctgctgga
      601 gcaggatttt tacgccagtg gtccgaacca gaagtgggcg ggagacatca cgtacttacg
      661 tacagatgaa ggctggctgt atctggctgt ggtcattgac ctgtggtcac gtgccgttat
      721 tggctggtcc atgtcaccac gcctgacggc gcaactggcc tgcgatgccc tgcagatggc
      781 gctgtggcgg cgtaagagac cccggaacgt tatcgttcac tcggaccgag gaagtcagta
      841 ctgttcagca gattatcagg cgctgctgaa gtggcataat ctgcgtggca gtatgagcgc
      901 aaagggctgc tgctacgata atgcctgcgt ggaaagcttc tttcattcgc tgaaagtgga
      961 atgtctccac ggagaacact ttatcagccg ggaaataatg cgggcaacag tgtttaatta
     1021 tatcgaatgt gattacaatc gctggcgacg gcacagttgg tgtggcggcc tcagtccgga
     1081 acaatttgaa aaccagaacc tcgcttaggc ctgtgtccat attacgtggg
//
LOCUS       DAZPAC010000499         1129 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZPAC010000499 DAZPAC010000000
VERSION     DAZPAC010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.512"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            170..625
                     /gene="csgB"
                     /locus_tag="ACHUPM_004788"
     CDS             170..625
                     /gene="csgB"
                     /locus_tag="ACHUPM_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR0872303.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            666..1118
                     /gene="csgA"
                     /locus_tag="ACHUPM_004789"
     CDS             666..1118
                     /gene="csgA"
                     /locus_tag="ACHUPM_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR0872304.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          334 a          227 c          273 g          295 t
ORIGIN      
        1 tttggtatga acaaaaaaag aaaaatacaa cgcgcgggtg agttattaaa aatatttccg
       61 cagacatact ttccatcgta acgcagcgtt aacaaaatac aggttgcgtt aacaaccaag
      121 ttgaaatgat ttaatttctt aaatgtacga ccaggtccag ggtgacaaca tgaaaaacaa
      181 attgttattt atgatgttaa caatactggg tgcgcctggg attgcagccg cagcaggtta
      241 tgatttagct aattcagaat ataacttcgc ggtaaatgaa ttgagtaagt cttcatttaa
      301 tcaggcagcc ataattggtc aagctgggac taataatagt actcagttac ggcagggagg
      361 ctcaaaactt ttggcggttg ttgcgcaaga aggtagtagc aaccgggcaa agattgacca
      421 gacaggagat tataaccttg catatattga tcaggcgggc agtgccaacg atgccagtat
      481 ttcgcaaggt gcttatggta atactgcgat gattatccag aaaggttctg gtaataaagc
      541 aaatattaca cagtatggta ctcaaaaaac ggcaattgta gtgcagagac agtcgcaaat
      601 ggctattcgc gtgacacaac gttaatttcc attcgacttt taaatcaatc cgatgggggt
      661 tttacatgaa acttttaaaa gtagcagcaa ttgcagcaat cgtattctcc ggtagcgctc
      721 tggcaggtgt tgttcctcag tacggcggcg gtaaccacgg tggtggcggt aataatagcg
      781 gcccaaattc tgagctgaac atttaccagt acggtggcgg taactctgca cttgttctgc
      841 aaactgatgc ccgtaactct gacttgacta ttacccagca tggcggcggt aatggtgcag
      901 atgttggtca gggctcggat gacagctcaa tcgatctgac ccaacgtggc ttcggtaaca
      961 gcgctactct tgatcagtgg aacggcaaaa attctgaaat gacggttaaa cagttcggtg
     1021 gtggcaacgg tgctgcagtt gaccagactg catctaactc ctccgtcaac gtgactcagg
     1081 ttggctttgg taacaacgcg accgctcatc agtactaata catcatttg
//
LOCUS       DAZPAC010000500         1124 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZPAC010000500 DAZPAC010000000
VERSION     DAZPAC010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.513"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..478)
                     /gene="umuC"
                     /locus_tag="ACHUPM_004790"
     CDS             complement(<1..478)
                     /gene="umuC"
                     /locus_tag="ACHUPM_004790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR0872305.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
     gene            complement(478..897)
                     /locus_tag="ACHUPM_004791"
     CDS             complement(478..897)
                     /locus_tag="ACHUPM_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR0872306.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT          318 a          293 c          236 g          277 t
ORIGIN      
        1 tctgccgctg ccattttttt gccgcatgat tggcaagctt agccagcgtt ttggtctggg
       61 cgatccccat accaacagta agatgggtac gttgtagcac cgttgcgcga atttctctgc
      121 caaaatcagt cagatcgcga caattacgca cacctgtcag atcgcagaat gcctcatcaa
      181 tactgtaaat ctcgacgcgg ggcgatagtt cttccagcgt cgacatcacc cgattgctca
      241 tgtctgcgta aagctcataa ttgctgctaa agcaaaccac accacagcga cgaaacagat
      301 ctttttgttt gaaccaggga tcgcccattt taacacccag cgcctttgcc tcagcgtttc
      361 gggcgataac gcaaccgtca ttattcgata gcacaaccac cggtttaccc cataaatcag
      421 ggcgaaacac cgtctcacag ctggcataaa acgcgtttac atcacagaga gcaaacatca
      481 gcgcatcgcc ttaacgacgt ggatcaccac accaaagaca tccagcgtat cttcgctact
      541 gatggtaatg ggcgagtacg cactgttcat gggaataagc tgtaccgtcg ggcgtagttg
      601 caattttttc accgtaaact cgccgtcaac agcagcgatg acaatatcac catggctggc
      661 ggtaatagcg ctatcgacaa tcagtaaatc accgtcacta attccaccat caatcataga
      721 atcaccactt gctttgacga agtaagtcgc gctgggatgc tggatcaaca gttgattcag
      781 atcgatgcgc tgttcgacgt aatctgctgc cggtgaagga aagccacact gaacaagatc
      841 gctaaatagc ggaaaagtca caatttcgcg gagatccgca ggcttgataa acaacataat
      901 aatctgcctg aagttatact gtttttatat acagtaatct gttcttgcca gcagatcaat
      961 actgattcag gctatcaata tttgtcgctg catagactgc tgatttttcg ttctcttatc
     1021 ctgtgctcac gtggccttct ggcgacgacg ctcatccagc agaaatgaaa aatatcaccc
     1081 ggcaaaaaaa tagaatagaa gcattcgcca taatgacatt aaac
//
LOCUS       DAZPAC010000501         1120 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZPAC010000501 DAZPAC010000000
VERSION     DAZPAC010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.514"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..1049
                     /locus_tag="ACHUPM_004792"
                     /pseudo
     CDS             1..1049
                     /locus_tag="ACHUPM_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          439 a          215 c          168 g          298 t
ORIGIN      
        1 ataccgataa tgctaaaacg ccagacacac cgaagtggcc ttgcgataat tgggaagaac
       61 aacaacccat taattccaca ttctctggag aaggatatat ctctgatcaa tatgatttag
      121 tgcaacacca acttcaacaa attaatgcgt gccacacgaa cactacatat acaaatgcag
      181 actactcaaa agttgtggct caacttgtaa gtcttattac taacattgaa acgataagtt
      241 cgacacaact tacgcaacag acacaatcaa tacttaatca gattaataac attagatatg
      301 aaaaaaacaa aagtgctgaa tgtcgaatca ttgttgtcgc taatcctaaa cccgacaagg
      361 caatcataac taaaattagc gtagaagagg gaatacccat aacattcagt gtccaaacca
      421 tgttttcaga tactaatttt attgctgaac aacgagctga cttaccaact aatataaaag
      481 atattcaaag cctgtatcaa aaaatgacga agttatatat tgagcacagt gaaaataaaa
      541 acaggatgaa agtctttgcc ggagccaact ttattgattt caatatgaca ggacagaacc
      601 tgtccgggtt cgttctaacc ttgtcaaggt tttatttcga agatctactt aatataaatt
      661 tcactgacgc aaatttaggt gacactattt tttacataaa gaacacccta cccccaaatt
      721 atataaagat ggacaatatc ttgacaaaca aatcgaaggt ttattttcaa cattattaac
      781 cattaatgac aactttttac gagcaaaagc ggaaattgct tcgacaataa ttaaattttt
      841 agaggccagg ataaccaatc tttcatacaa tgacatatta aaatatcagc aagaattcca
      901 aaaacaatgt tataagcaag ttaaagcctt tacgacactc tctcgataca ataaaattca
      961 aacatgggca gaaatgtctg aatatcagtt cgaagtattt caatacgaga cgcttaaccc
     1021 caaaaaaatg tcacacacgc cttatttaaa gaggccactt cctaatgaaa aagatataaa
     1081 ttatggggtg gaaatagaaa taccatctgg caaaagaatt
//
LOCUS       DAZPAC010000502         1118 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZPAC010000502 DAZPAC010000000
VERSION     DAZPAC010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.515"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            3..677
                     /gene="tssD"
                     /locus_tag="ACHUPM_004793"
                     /pseudo
     CDS             3..677
                     /gene="tssD"
                     /locus_tag="ACHUPM_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            657..>1118
                     /locus_tag="ACHUPM_004794"
     CDS             657..>1118
                     /locus_tag="ACHUPM_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872307.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPELAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTGFAPIFPDICYHLTHYKPAA"
BASE COUNT          338 a          227 c          249 g          304 t
ORIGIN      
        1 atattattta cctgaccata accggagaac aacaaggatg tatttcctct cgttgtggaa
       61 cgagcgcatc aataggtaat cgctggcaga ttggccatga tgatgaaatc ttcgcgttct
      121 cgctctctaa tagtattacg aatacaggca aagggtcata attgcatggc cttagtttct
      181 gtaaattgat tgacaaaagc tccccgttac ttattaacgc tattaacaat aacgagcagt
      241 tgttcatgga gtttgacttc tatcggatca ataggtttgg tcgatgggag aaatattaca
      301 acattcaact ccgcggcgcg ctcttatcag caatcaatca cctgtttact gagaacaatc
      361 tggatacaga agccatcact gtcagttatt aatatatact ctccaggcat ctgattgcta
      421 atactgaatt tagttattgg gcatttcccg ataactacaa ccgtctgttt ataccacggc
      481 caaaaactaa agctggtaat ggtttcaaaa cacttaatag taaagcggtt ggcagactgc
      541 ttgctgctgt cggcatatac aatgggaata tcgaaggatt cagggaaaca gcagacaagt
      601 tggggggaga tgcacctgct ggttatgatc aatttatgga taacaaaggg ttactcatcg
      661 ctggtgcttc aatagttgct ggagcagttc tggggagaat gagactccct gatttggaat
      721 cacttgagca tttcggagcc agaggagcag tatcaggaag accatttaat cccgagcttg
      781 ccggtggtcc aatagaaaac ctgacgacag atggcgtaac aatcaacagg gaaggtatcg
      841 ctattgttga aaaacatata gccagatttg gtcatgatcc tgtaaatgaa gtgatgatca
      901 atagactgaa agacattgag aaaggaaaaa tccccccaga acaagtagat cttaacttct
      961 atactcatga gtgtcgggaa tatcagcgtt actgtaatct tggttgggaa acaggacaac
     1021 ctgatggtga cgcaggctat gcactctgga atcatactgg atttgcccct atatttccag
     1081 acatctgtta tcacttaacc cattacaagc ccgctgcc
//
LOCUS       DAZPAC010000503         1117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZPAC010000503 DAZPAC010000000
VERSION     DAZPAC010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.516"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            84..1115
                     /locus_tag="ACHUPM_004795"
     CDS             84..1115
                     /locus_tag="ACHUPM_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR0872308.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAZPAC010000504         1099 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZPAC010000504 DAZPAC010000000
VERSION     DAZPAC010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.517"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..931
                     /gene="lpxM"
                     /locus_tag="ACHUPM_004796"
                     /gene_synonym="msbB"
     CDS             <1..931
                     /gene="lpxM"
                     /locus_tag="ACHUPM_004796"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0872309.1"
                     /translation="SEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDPVLAKI
                     GRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELAIRGPE
                     KFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFHQQRNP
                     VIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFAT
                     YKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMIARQMN
                     KTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT          307 a          220 c          258 g          314 t
ORIGIN      
        1 atccgagttt attcctgaat ttaagaaaaa ttatctttcc cctgtttact ggtttacatg
       61 gttcgttttg ggaatgattg caggtatttc aatgtttccc ccttcattca gagatcctgt
      121 cttggccaaa atagggcgtt gggtgggcag attgagcaga aaagctcgtc gcagggcgac
      181 gattaattta tcgctttgtt tcccggaaaa gagtgataca gaacgggaaa taattgtcga
      241 caatatgttt gccacagcat tgcaatccat agtgatgatg gcagaactgg caattcgtgg
      301 tccggaaaag ttccagaaac gagtgttctg gaaaggcctc gaaattcttg aggaaatccg
      361 gcacaataac agaaatgtga tttttctggt tccccatggc tggagcgtgg atattcctgc
      421 aatgttgctg gcagctcagg gggaaaaaat ggccgccatg tttcatcagc aacgaaatcc
      481 agtgattgat tatgtctgga attctgtacg gcgtaaattc ggggggcgct tacattcccg
      541 ggaggatggg ataaaaccat ttattcagtc agtacgccag ggatactggg ggtattacct
      601 tccagatcag gatcatggtc ctgaatacag tgaatttgct gatttttttg cgacctataa
      661 agcgacatta ccaattattg gacgtctgat gaacatcagt caggctatga ttataccgct
      721 tttcccggtt tatgatgaaa aaaaacattt cctgactatt gaagttcggc caccaatgga
      781 tgcatgcatt gccagcgcgg acaataaaat gattgcccga caaatgaaca aaacagtgga
      841 aattttggtg gggtcacatc cggaacagta tatctgggtt ttaaaattgt taaaaacgcg
      901 caaatcaaac gaagcggacc cgtacccttg aaattataaa gtacaggtat ttccactagt
      961 tgtttcttac aggttaccaa tcgaaacaca tcccccttcc gtgtgttgcc cgtttggcac
     1021 ggaggactgt gtagatgcag ttcgttaaat ttaatttatg cctaaatagc tgcgcttaat
     1081 accgctacac ttttgccag
//
LOCUS       DAZPAC010000505         1096 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZPAC010000505 DAZPAC010000000
VERSION     DAZPAC010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.518"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1074
                     /locus_tag="ACHUPM_004797"
     CDS             join(<1..167,167..1074)
                     /locus_tag="ACHUPM_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFR0872310.1"
                     /translation="EGASRLSLPEGTLGQWVTAARKGLGTPGSRTVAELESEILQLRK
                     ALNEARLERDIFKKSNSVFCTGVAEKYALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQ
                     HEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRL
                     RCKQKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIK
                     DVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQE
                     QSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQ
                     RRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          312 a          261 c          286 g          237 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      601 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      661 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      721 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      781 gatcgaggtt cacagtactg cgcatacgat taccgggtca tacaggagca gtctggtctg
      841 aaaacatcaa tgtcgcgtaa aggtaactgt tacgacaacg ctccgatgga aagcttctgg
      901 ggaacgctga aaaatgagag cctgagccac tatcgtttta ataaccggga tgaagccatc
      961 tcagtaatac gggaatacat tgagattttc tacaatcgtc agcgtcgtca ctctcgtctg
     1021 gggaatatct ccccggcagc cttcagggaa aaatatcatc agatggctgc ttaaaaaaag
     1081 aacaaatggt agtgtc
//
LOCUS       DAZPAC010000506         1085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZPAC010000506 DAZPAC010000000
VERSION     DAZPAC010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.519"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..643)
                     /locus_tag="ACHUPM_004798"
     CDS             complement(<1..643)
                     /locus_tag="ACHUPM_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0872311.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="ACHUPM_004799"
     CDS             complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="ACHUPM_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR0872312.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          312 a          247 c          191 g          335 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaa
//
LOCUS       DAZPAC010000507         1070 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZPAC010000507 DAZPAC010000000
VERSION     DAZPAC010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.520"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            10..>1070
                     /locus_tag="ACHUPM_004800"
     CDS             10..>1070
                     /locus_tag="ACHUPM_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFR0872313.1"
                     /translation="MKNFIPFALPEIGEEEIAEVIDSLRSGWITTGPKAKQFEQEFSN
                     YLGANVQSLAVNSATSGLHLALEAVGVKPGDQVIVPSYTFTATAEIVRYLGADPVIVD
                     VDRKTFNISVDAIEKAITNETKAIIPVHFAGLACDMDSILSIAKKYDLKVVEDAAHAF
                     PTTYKGSKIGTLDSDATVFSFYANKTMTTGEGGMVVSKNKDIIERCKVMRLHGISRDA
                     FDRYQSKTPSWFYEVVAPGFKYNMPDICAAIGIHQLRKIDDFQKKRQRMAKIYDDALK
                     ELPLELPEWPTNASDIHAWHLYPIRLKTDSAINRDDFIKKLSDLGIGCSVHFIPLHKQ
                     PVWRDTYNLNASDFPVSEE"
BASE COUNT          319 a          187 c          225 g          339 t
ORIGIN      
        1 gtattttaaa tgaaaaattt tattcctttt gcgttacctg aaattggcga agaagaaatt
       61 gcagaggtaa ttgactcttt acgttcaggt tggattacga caggtcctaa ggctaagcaa
      121 tttgaacaag aattttctaa ttacctagga gcgaacgttc aatcattagc tgttaactct
      181 gctacgtcgg gcttacattt ggctcttgaa gctgttggcg taaagccggg agaccaagtt
      241 attgtcccat catatacatt cactgctact gccgaaattg tcaggtacct tggtgctgat
      301 cctgtaattg ttgatgtaga tcgtaaaaca tttaatatat cagttgatgc cattgagaag
      361 gctattacta atgaaacaaa ggcgattatt ccagtacact tcgctggatt agcttgtgac
      421 atggattcaa tcttatcaat tgctaaaaaa tatgacctaa aggttgtcga ggatgccgct
      481 catgcatttc ctacaacata taaaggaagt aagataggaa cgcttgattc agatgctacg
      541 gtttttagct tctacgccaa taaaactatg acaaccggtg aaggcggaat ggttgtttca
      601 aaaaataaag atataattga gcgttgtaag gtaatgcgtt tacatggaat cagtcgtgac
      661 gcttttgacc ggtaccagtc taaaactcct tcttggtttt atgaggttgt agctccaggg
      721 tttaaataca atatgcctga tatctgtgcg gcaatcggta ttcatcaact tagaaagatc
      781 gatgattttc agaaaaaacg tcaacgaatg gcaaaaattt acgatgatgc gttaaaagaa
      841 ttgccacttg aattgcctga atggcctact aatgctagtg atattcatgc ttggcatcta
      901 tatcctatcc gcttaaaaac tgattcggct attaatcgcg atgattttat taagaagtta
      961 tcagatcttg gaattggttg ttctgtccat tttataccgt tgcataagca accggtttgg
     1021 cgtgatacat ataatttgaa cgccagtgac tttccagttt ctgaggagtg
//
LOCUS       DAZPAC010000508         1067 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZPAC010000508 DAZPAC010000000
VERSION     DAZPAC010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.523"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACHUPM_004801"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACHUPM_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR0872314.1"
                     /translation="MPIMLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          322 a          173 c          245 g          327 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tatgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg gggcaatgaa
      721 aagttattat ttatcggtaa catgatgggc atccagttaa agccggtgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taataaggat aaacaaaatt ggctgtaaaa aaacgatact gtttcagtta
      901 accgtgaatg gcctgtttgc accgtatgtg acagtaatac gctgcttcat gataagccct
      961 caaaaacata cttatgatat gtatatgagt atatattaat ggtacaaacc atcaatatcc
     1021 ataaaaaata caccattaat tcgctataag tttttaatac aattttt
//
LOCUS       DAZPAC010000509         1063 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZPAC010000509 DAZPAC010000000
VERSION     DAZPAC010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.524"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            222..350
                     /locus_tag="ACHUPM_004802"
                     /pseudo
     CDS             222..350
                     /locus_tag="ACHUPM_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            540..944
                     /locus_tag="ACHUPM_004803"
     CDS             540..944
                     /locus_tag="ACHUPM_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872315.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
BASE COUNT          325 a          232 c          266 g          240 t
ORIGIN      
        1 acaaagtgaa ttaaatgaat atataatgcg ttcgaagtat atgtattgag ctgtcatact
       61 gagaaacaaa ccttagggca gcacaattaa agccagaaac aactatgcaa gaaagttact
      121 aaataagtgc tcggaacagt aaaacactca ggggggactc agtccggatt atgtaatcag
      181 ataaattgcc aaacatcaca aatcaccagc cggactgagt gatgccgagc attgcaccaa
      241 taccccgtga tgaacgatgc ctgatgcaga aagctcccta taaaacaaag aacaaaaact
      301 acgaccggcg attcaccgtc atactgatgc tgcatcgtgg tgacactgtc tgtcactttc
      361 atgttgactg aacaactctg ctgcgaagaa ggtgtaccgc cggcgggatt tgattgtccg
      421 gcgtctagct tccacttccc tgatggaact gaaagttgtt atggccggtc agcttgccct
      481 gtccgcagga catgaggtgc tgtgacggac agcaccagta acggaaagtg acaatgaaaa
      541 tgaatactgc gctgatagtt gcactaatgt gtatgtggta tgcggtaccc gctgctgcaa
      601 aagagacttt actggctatg ccacgtaatt caacagaaca ttgctatgcg gagattaatg
      661 tgcacggacc atatggggtg tatttccgag tggtcccgca tccccccggg ggaaagagct
      721 gggtcgaatg taacagtgac tactattact ctgataaacc accgggagta cagatactcg
      781 gaacccgtgc agggtgtcgg gtatacggaa tctgcgggac cacctcgaca ctgcatgtgg
      841 ctggaagagg ggttgtgtgt attaaaaata tatgcagtcc acgtgggatg ataattcaca
      901 ggatacggaa acgtcctgta gtggctgtaa gcgatgagat gtgatcttac ccagcaatag
      961 tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacatga caaaaacagt
     1021 atcaaccagc aaaaaaacac gtaaacagca ttcgcctgaa ttt
//
LOCUS       DAZPAC010000510         1047 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZPAC010000510 DAZPAC010000000
VERSION     DAZPAC010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.525"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            108..257
                     /locus_tag="ACHUPM_004804"
                     /pseudo
     CDS             108..257
                     /locus_tag="ACHUPM_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            284..388
                     /locus_tag="ACHUPM_004805"
                     /pseudo
     CDS             284..388
                     /locus_tag="ACHUPM_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            728..985
                     /locus_tag="ACHUPM_004806"
     CDS             728..985
                     /locus_tag="ACHUPM_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFR0872316.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          357 a          180 c          198 g          312 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tattgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 ccattagtct gcttcctgtt cttacaataa tcagtattat gtattttatt tatatagcaa
      661 aatatgcttg ttacacctgt cataattagg catggtaaaa ttattgactg aaccctgggg
      721 gaggaagatg cacagtgatt attataatat ggtttttggt gaaaaattgg ccaatatttt
      781 atatgaggct aactctcaat ttttttacga gagaaatgtc atagaagagg cagtcaacgc
      841 tttattctgt gagcgtgaaa taataaacaa caagaacata ataaagaagc tgatgttctt
      901 tttgtcagat gttaatcaca ccaagaaaga tgttgttcag agcgcactta atattatcat
      961 tgacattaca tcaggtgata tttgagattt tacataaaag ataatctctc aaggagtctt
     1021 atctgaaaac gaaatatata gtccctc
//
LOCUS       DAZPAC010000511         1041 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZPAC010000511 DAZPAC010000000
VERSION     DAZPAC010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.526"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(91..1041)
                     /locus_tag="ACHUPM_004807"
                     /pseudo
     CDS             complement(91..1041)
                     /locus_tag="ACHUPM_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          223 a          254 c          209 g          355 t
ORIGIN      
        1 tgtactccaa tgactagtct aaaaactagt attaagacta tcacttattt aagtgatact
       61 ggttgtctgg agattcaggg ggccagtcta ctgtgtttct gttagccatt gtccggcggc
      121 tttttgctgg gtaatgcttg cctgcacctc tctggctgat ctttgccaat atttagaagt
      181 taagaggagg tcgtccgcta aatcttccag acgactaccg atctgtgcgt cggtatagcc
      241 ctttttcacc attccgttga cagcttttga aattaaacca gcagagttat tggcaaagtc
      301 tttcaaatcc tttttagtta agaatccttc attcattaaa gtgttaatac cagaatcacc
      361 gcccttccat atgcctttcc ctgttttctt atcaactaca tattcagcct cgtcatagct
      421 tttggtagcg cgttccttta catctttacc gatggcatct agtatttttc tgcgtcccgg
      481 atcaccctgg aggctatcga tcccgttgtc atacgcccaa cgctcaagat tattaagtaa
      541 gttgtaatca actccaccaa gccttgcacg ttggattagc tccccttctg tttcagcatt
      601 agcaaagctg ttagccataa attcagaggt tttactaaca gccatcacgc cagcagcagc
      661 cgtagcgcct ccaaataaca gagcgcccat cgtggcggaa ttccctgatc ctcccttaac
      721 gggggcaaat tgagcttttc tgttgccatt aatcttacgt tgttgctgtt gtaggcgttt
      781 catctgacta ttcatacgcg ccaggctgat agccccttgc tcatattgtt ttgctatctc
      841 tctagcctgt gcaatagctt tgtattgttc cgctacagag agacggtgca ttgcactgat
      901 agagcttgat acatcaagga gttttaattc tgctttctct ttacgtgctg ctatttgttg
      961 ctgttgcctt gcctgtgctc ttgctgcggc agcttgtgct ctttgattct gcttttctgt
     1021 tttagctttc tgttgagcaa t
//
LOCUS       DAZPAC010000512         1023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZPAC010000512 DAZPAC010000000
VERSION     DAZPAC010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.527"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(77..865)
                     /locus_tag="ACHUPM_004808"
     CDS             complement(77..865)
                     /locus_tag="ACHUPM_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0872317.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="ACHUPM_004809"
     CDS             complement(905..>1023)
                     /locus_tag="ACHUPM_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR0872318.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAZPAC010000513         1019 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZPAC010000513 DAZPAC010000000
VERSION     DAZPAC010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.528"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            133..562
                     /locus_tag="ACHUPM_004810"
                     /pseudo
     CDS             133..562
                     /locus_tag="ACHUPM_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            743..892
                     /locus_tag="ACHUPM_004811"
                     /pseudo
     CDS             743..892
                     /locus_tag="ACHUPM_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          252 a          211 c          308 g          248 t
ORIGIN      
        1 tcctgccggt atcagcgccg acggcatgta ttgccctgaa tgcggtacgg ttcactggcc
       61 ggatggcgtt attccgcctt tctgatgctt ccccgttttg ccgatatttt tcagcaggga
      121 aaccgatggc ttaactggct ggagaagcag tcggtgcaga tgagccgcct ggaacattat
      181 gccggtcagg atgaaattgg cctgcgttac aacagccacc ggacaaaacg agaagaaaac
      241 cttgtgatga gtggcgacga gtttatggaa aggttctcct ggcatgtggc ggataagggg
      301 ttccggatgg tgatacgggg tcctgagtcc ggtgaagcgg cgattactgg aagatgtggt
      361 gtacgccata acggagacag tgagaaaaac ggcgaggcaa atcacaagga gagggatgta
      421 tcagcagtga ctgaaggttg acccgctgaa gtgcgagctg tgcggaggtc agatgcggtt
      481 tacggggctg aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc
      541 tcgaatacgg tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa
      601 cggagcaaaa aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct
      661 ataccgatta cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt
      721 aatcttttgg ggatttaaat tcctatcgat ggacatgaat gcaggctata taagagcagc
      781 gcgcatccac ttacccaatg cggttgagaa aatagccttc gatcgctttc atgtggcgaa
      841 acaaccgggt gaggtggtgg ataaaacccg ccagaatgaa ccgccccggg tttcctggag
      901 agtgttttat ctgtgaactc aggctgccag atcatcgttt ccgatggaag cataataagc
      961 tttttctgct tctgccggag gagtatggcc cagcctttcc agcaatcgtc gattgttat
//
LOCUS       DAZPAC010000514         1012 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZPAC010000514 DAZPAC010000000
VERSION     DAZPAC010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.529"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            24..971
                     /gene="icsP"
                     /locus_tag="ACHUPM_004812"
     CDS             24..971
                     /gene="icsP"
                     /locus_tag="ACHUPM_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFR0872319.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIDYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
BASE COUNT          344 a          175 c          205 g          288 t
ORIGIN      
        1 caaccaaaaa agtagagttc tcgatgaaat taaaattcct tgtacttgca ctttgtgtac
       61 cagcgatctt tactacacat gctaccacta actatccact tttcataccg gacaacatca
      121 gtacggatat tagtctagga tctctgagtg gcaaaacaaa agaacgcgtt tatcatccca
      181 aggaaggggg gagaaaaatt agtcaactgg actggaaata cagtaatgcg actattgtta
      241 gaggtggcat cgattggaag ctaattccaa aagtgtcttt cggagtttcc ggttggacta
      301 ctttaggtaa ccagaaagca agcatggttg ataaagactg gaacaattcc aatacccctc
      361 aggtatggac cgaccagagc tggcatccca atacgcatct ccgtgatgct aacgaattcg
      421 agctgaatct taaaggttgg ttattaaata atttggatta tcgacttgga ctaatagcag
      481 gttaccaaga gagtcgttac agttttaatg caatgggagg gagttatatt tatagtgaaa
      541 acggggggaa caggaataaa aaaggggcac atcctagtgg tgaaagaaca atagattaca
      601 aacagctctt caaaatacct tatattggat taactgctaa ttaccgccat gagaattttg
      661 agtttggagc agaactgaaa tatagtggtt gggttcgttc atctgataca gataaacact
      721 atcagactga gacaattttt aaagatgaaa taaaaaacca aaattactgc tctgttgctg
      781 cgaatattgg atactatgtc acccccagtg caaaatttta tatagaaggc tccagaaatt
      841 acatttctaa taaaaaaggt gatacatctc tttatgagca aagtaccaat atatctggca
      901 ccattaaaaa tagtgcaagt attgaatata ttggttttct cacttccgca ggtataaagt
      961 atattttttg aattggttca ccgagttgat tacgttttca tggctaaaaa tt
//
LOCUS       DAZPAC010000515         1000 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZPAC010000515 DAZPAC010000000
VERSION     DAZPAC010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.530"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>1000
                     /locus_tag="ACHUPM_004813"
     CDS             <1..>1000
                     /locus_tag="ACHUPM_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HFR0872320.1"
                     /translation="SGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTG
                     SVTVSAVTTAGGMQTVDITLVAGPADASKSVLKNNRSSLKGDFTDSAELHLVLHDISG
                     NPIKVSEGLEFVQSGTNVPYVQVSAIDYSKNFSGEYKATVTGGGEGITTLIPVLNGVH
                     QAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAA
                     DYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSYVYEIRVKSWWVNAGEA
                     FMIYSLAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDMGHYTTEAGFQSNMYW"
BASE COUNT          293 a          220 c          253 g          234 t
ORIGIN      
        1 agtggtaacc tgatcgaagg tctcactgtg tactttgcct taaaaagcgg ctctgccaca
       61 ttaacgtcat taacagcggt gaccgatcaa aacggaatcg cgacaacaag cgtgaaagga
      121 gcgatgacag gtagcgtcac ggtaagcgca gtcacgaccg ctggtggaat gcaaacagta
      181 gatataacgc tggtggcagg cccagcagac gcctcaaagt cagtccttaa gaacaatcgg
      241 tcatcattga aaggagactt taccgatagt gctgagctac atcttgttct gcacgatata
      301 tcaggcaatc cgatcaaagt ttctgaaggg ctggaatttg tacagtcagg taccaacgtg
      361 ccctatgtgc aagttagtgc aattgactac agtaaaaatt tctcaggcga gtacaaagcc
      421 actgttacag gaggcggaga gggtatcaca acgctgatcc ctgtattgaa tggtgttcat
      481 caagctggtc tgagtaccac aatacaattc actcgcgcag aagacaaaat aatgagcggt
      541 acagtatcag tcaatggtac tgacctaccg acaactacat tcccttcgca ggggttcacc
      601 ggggcgtatt atcagttgaa taatgacaac tttgccccag gaaaaacggc ggctgattat
      661 gagttttcaa gctctgcctc ctgggtcgat gttgatgcta ccggtaaagt gacatttaaa
      721 aatgtcggca gcaattcgga aaggattacg gcgacaccaa aatcaggagg ccctagctat
      781 gtatacgaaa tccgtgtgaa gagttggtgg gtgaacgccg gcgaggcttt catgatatac
      841 agccttgctg aaaatttttg cagcagcaat ggctacacgc tccccagagc aaactattta
      901 aaccacagta gttcccgagg catcgggtca ctgtacagtg aatggggaga tatggggcat
      961 tacacgactg aagctggttt tcaatcaaat atgtattggt
//
LOCUS       DAZPAC010000516         1000 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZPAC010000516 DAZPAC010000000
VERSION     DAZPAC010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.531"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..617
                     /locus_tag="ACHUPM_004814"
                     /pseudo
     CDS             1..617
                     /locus_tag="ACHUPM_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
     gene            614..>1000
                     /locus_tag="ACHUPM_004815"
     CDS             614..>1000
                     /locus_tag="ACHUPM_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFR0872321.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYR"
BASE COUNT          356 a          172 c          152 g          320 t
ORIGIN      
        1 tccggaacaa aaatatcaca acgtgaatgc actgatattt tatttgcaga aatgaaagaa
       61 ctagcaaaaa tgttttcctt ttttggacaa tataaaacac ttatcgaaga tctgattgaa
      121 cattttagat atggaaatgg cagcaatttt catagccaac aattaaattt atcttttcat
      181 gaaaaaataa atatggttat aatagtccaa tacgaataat caaagaatgt atcgaaaatg
      241 gcattaattc aactccctcg acaggatacc aacctcttat cctgcaaagc attaaaacca
      301 aattactttc atcaagacta aataaattta atgacttcga ggacagtttt aatggcttag
      361 gaatcagtgt tcatgatatt tcagctcaaa aaatatctct tttaagtttt caaaaatacg
      421 ctataggatg gagtgcaaca attcattttg tagcacagga ccacttcggt cttgatgtaa
      481 ctgatatcaa aaacaagact tacagcaaat atagattttt tcgcatatgg ttctttttac
      541 aacggcataa agactttgct ttcaaacctt ttttcaccaa ttttaacact atcgaaagaa
      601 tcgaaaatta tctatgaaaa gcaaactcca gaaaattata ttatgtttat ttcttctatg
      661 ctgcatctat aatttatgga cattgcgtcc tgtacagatt ttgtatacat actctgatgc
      721 tggcaattca gtctttctgg tcgttgatca tctgccatgg actgacagcg ataaaataaa
      781 ttggtatctg aagcatcaga atgaaattaa aaatcaacat cccctaccag aaggtagttg
      841 gcatacctgg tatgtcattg atatcggtaa tggcttcact gactataaaa aatatatcga
      901 agggccgtat gaagatctct actgtttccc gacaattaag agcaatgata attgcatcgt
      961 gaaaaattac ttaatggtta ttaatgaata cccttatcgt
//
LOCUS       DAZPAC010000517          998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZPAC010000517 DAZPAC010000000
VERSION     DAZPAC010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.532"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..93)
                     /gene="yqcG"
                     /locus_tag="ACHUPM_004816"
     CDS             complement(<1..93)
                     /gene="yqcG"
                     /locus_tag="ACHUPM_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFR0872322.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
     gene            232..903
                     /gene="queE"
                     /locus_tag="ACHUPM_004817"
     CDS             232..903
                     /gene="queE"
                     /locus_tag="ACHUPM_004817"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR0872323.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          276 a          213 c          230 g          279 t
ORIGIN      
        1 gttatcatta aaaactaata cagaaaaaat aaatataatt ttggtgaatg ttttgacatg
       61 attaaatcca ttttctttat tttcttctga cataatacgt tactcccttt aattattaac
      121 gatatgtttc gcatttttct ttaaatctgc ctgtaacaga atgttaataa gacatgattg
      181 ttgtggcagg atctgcaata attaacaatt atccacttca cagagaatgc tatgcagtac
      241 ccgattaacg agatgttcca gaccctgcaa ggtgagggtt actttaccgg cgtccccgcc
      301 atttttattc gtttacaggg atgcccggtt ggctgtgcct ggtgcgacac caaacacacc
      361 tgggaaaagc ttgaggatcg ggaagtctcc cttttcagca ttctggcgaa gaccaaagag
      421 agtgataagt ggggggcggc gagcagtgaa gacttgttgg ctgtcattag tcgccaggga
      481 tacaccgcgc ggcatgtggt gattacgggt ggtgagcctt gcattcatga tttgctacca
      541 ctgactgatc tgctcgaaaa gaacggtttt agctgccaga tcgaaaccag tggtactcat
      601 gaggtacgct gcacaccgaa tacctgggtt accgtatcgc caaagctgaa catgcgcggc
      661 ggctatgacg tgttgtcaca ggcactggag cgagccaacg aaatcaagca tccagtgggg
      721 cgcgtacgcg atattgaagc actggatgaa ctattggcaa cgctgaccga tgataaaccg
      781 cgagtcattg cactgcagcc gattagccaa aaggatgatg ccacacgttt gtgcattgaa
      841 acctgcattg cgcgtaattg gcgtttgtcg atgcaaacac ataaatatct aaatattgcc
      901 tgattaaaca tttataagcg ttataaatgg gtggaacctg taaagacttc tactcattta
      961 tattgtttgt cgcctctgaa aactcctcca ttttaccc
//
LOCUS       DAZPAC010000518          985 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZPAC010000518 DAZPAC010000000
VERSION     DAZPAC010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.533"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            43..303
                     /locus_tag="ACHUPM_004818"
     CDS             43..303
                     /locus_tag="ACHUPM_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872324.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            300..890
                     /locus_tag="ACHUPM_004819"
     CDS             300..890
                     /locus_tag="ACHUPM_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872325.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLPACHDAGMEVRVQINESAAP"
BASE COUNT          219 a          270 c          313 g          183 t
ORIGIN      
        1 tgatttttac ctgtcagaac attatcagat ggaacgtgca ggatggatca ggaaatgacg
       61 ttttcactca gctatgaaca actgacccgg tttgcggaaa aacgcatccg ggaatgcaat
      121 ctggacagcc acggcgtaac ttatctctgc gagtcagcca aagccggagc cgtgctgatc
      181 ttctggcatg aactggccat aaatggctat acctcaatga atgccataaa gagacaggag
      241 atcattgacg ccgaccatca gcgcctcaga aagctcatct ggccggagga tgactggaaa
      301 tgactcagag ccgccgaccg tcgccactgc agcggagagt gctgattgtg ctggctgccc
      361 ttgatgaaaa acggccagga cccgtgctga cgcgggacat agaacgggtg ctggagcaaa
      421 gcggggaagc cccggtctac gggccaaacc tgcgcgcctc ctgccgtcgt ctggaggatg
      481 ccggctggct gcgtaccctg cgcgctccga acctgcagct ggcagtggag ctgacggacg
      541 ccggccgggc cgtggcacag cctctgctgc tggcagaaca ggaccgcatc cgggcagaac
      601 agcgtgcggc agaagtcgtc gtactgccgc tggtcccggc agcaggtctg cccgcaggcg
      661 gcacttctgc cactgatctg gcggtggagc tgaacggcat aacgtatcag gcctgccgtg
      721 gggattttgt ggtgcgtctc gacggcagca cctgtctgca gttatggaat aaagaaggct
      781 gggtggtacg ccgggagggc gatccgctgg aagtggcgca gtggctgccg gcctgtcacg
      841 atgcaggaat ggaggttcgc gtacagataa atgaaagtgc cgctccgtaa gaacacgcct
      901 gtgcacggtt tacacatcat cccatggtgg cagtcggtta actttcctgc gccaccagtg
      961 gaaagaggcc atgagcagtc cggac
//
LOCUS       DAZPAC010000519          978 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZPAC010000519 DAZPAC010000000
VERSION     DAZPAC010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.534"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            15..>978
                     /gene="istA"
                     /locus_tag="ACHUPM_004820"
     CDS             15..>978
                     /gene="istA"
                     /locus_tag="ACHUPM_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFR0872326.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVSFD"
BASE COUNT          234 a          258 c          260 g          226 t
ORIGIN      
        1 attcaggaga gcttatggtc acttttgaga cagttatgga aattaaaatc ctgcacaagc
       61 agggaatgag tagccgggcg attgccagag aactggggct ctcccgcaat accgttaaac
      121 gttatttgca ggcaaaatct gagccgccaa aatatacgcc gcgacctgct gttgcttcac
      181 tcctggatga ataccgggat tatattcgtc aacgcatcgc cgatgctcat ccttacaaaa
      241 tcccggcaac ggtaatcgct cgcgagatca gagaccaggg atatcgtggc ggaatgacca
      301 ttctcagggc attcattcgt tctctctcgg ttcctcagga gcaggagcct gccgttcggt
      361 tcgaaactga acccggacga cagatgcagg ttgactgggg cactatgcgt aatggtcgct
      421 caccgcttca cgtgttcgtt gctgttcccg gatacagccg aatgttgtac atcgaattca
      481 ctgacaatat gcgttatgac acgctggaga cctgccatcg taatgcgttc cgcttctttg
      541 gtggtgtgcc gcgcgaagtg ttgtatgaca atatgaaaac tgtggttctg caacgtgacg
      601 catatcagac cggtcagcac cggttccatc cttcgctgtg gcagttcggc aaggagatgg
      661 gcttctctcc ccgactgtgt cgccccttca gggcacagac taaaggtaag gtggaacgga
      721 tggtgcagta cacccgtaac agtttttaca tcccactaat gactcgcctg cgcccgatgg
      781 ggagcactgt cgatgttgaa acagccaacc gccacggtct gcgctggctg cacgatgtcg
      841 ctaaccaacg aaagcatgaa acaatccagg cccgtccctg tgatcgctgg ctcgaagagc
      901 agcagtccat gctggcactg cctccggaga aaaaagagta tgacgtgcat cctggtgaaa
      961 atctggtgag cttcgaca
//
LOCUS       DAZPAC010000520          978 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZPAC010000520 DAZPAC010000000
VERSION     DAZPAC010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.535"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            44..978
                     /gene="yfdF"
                     /locus_tag="ACHUPM_004821"
                     /pseudo
     CDS             44..978
                     /gene="yfdF"
                     /locus_tag="ACHUPM_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530666.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
BASE COUNT          377 a          170 c          171 g          260 t
ORIGIN      
        1 gtaatatata tttgtaacaa atcaatcaaa atggaataaa atcatgctac catctatttc
       61 aatcaacaat accagcgcaa cttacccaga atccattaat gaaaataaca atgatgaagt
      121 taatggatta gtacaagagc tcaaaaacct ttttaatggt aaggaaggaa taagcacctg
      181 tattaaacat ctacttgagc ttataaaaaa cgccatacga gtaaacgacg atccttaaga
      241 tttaatatta ataattcctc agttacttat attgatattg gctccaatga tacagaccat
      301 attactattg gtatcgacaa ccaagaacca atagaattac ctgcgaacta taaagacaaa
      361 gaactcgtcc gtactatcat taatgacaac atagttgaga agactcatga tatcaataac
      421 aaggaaatga tcttcagcgc attaaaagaa atatatgatg gagatcctgg ttttatcttc
      481 gataagatat cacacaaact cagacatacg gtaacagaat ttgatgagag cgggaatagc
      541 gaaccaacgg acttatttac ctggtacggt aaagataaaa aaggcaactc tctcgctatt
      601 gtaattaaaa ataaaaacgg aaatgattac ttatctctcg gttactacga tcaggacgac
      661 taccacattc aaagaggaat tcgtattaat ggtgatagtc tcacccaata ttgtagtgaa
      721 aaagccagga atgcttcagc aatgcttcag cgtggtttga aagcagtaaa gctatcatgg
      781 cagaatcatt tgcaacaggt tccgatcatc aggttgtaaa cgagctcaac ggggaaagac
      841 tgagagaacc taacgaagtt tttaaacgtt taggtcgagc aataagatat aattttcaag
      901 tggacgatgc aaaatttaga cgcgacaatg taaaagaaat aatttctaat ttattcgcta
      961 acaaagttga cgttgacc
//
LOCUS       DAZPAC010000521          976 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZPAC010000521 DAZPAC010000000
VERSION     DAZPAC010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.536"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>976)
                     /gene="mxiA"
                     /locus_tag="ACHUPM_004822"
                     /gene_synonym="virH"
     CDS             complement(<1..>976)
                     /gene="mxiA"
                     /locus_tag="ACHUPM_004822"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFR0872327.1"
                     /translation="MGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAG
                     KIITTFGQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMS
                     IDADLKAGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGI
                     SVGMSQHGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNI
                     MSQIFGNPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSES
                     DSSGYTGTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDM"
BASE COUNT          356 a          214 c          141 g          265 t
ORIGIN      
        1 catatcattt gcatttatct tattttcggc aaataataaa attaaaggaa cggtttctga
       61 actaatagca tccagatttt ctatcattgc caaagatgag tcatggctat tatcaatatc
      121 aaatgtacca gtatagccac tagaatcaga ctcagacaga cttttttctt tttctacgac
      181 ctttttataa tagaataaag ccgtcaaagt aactgctatc aggaaaaaaa caaaaaacgg
      241 aaaacctggc aacatcccta tcgccaaggc aagagctgat gtaacaatga gaacaaaagg
      301 attcccaaat atctgggaca ttatgttacg tcccatatta tcgctatcac cattaactct
      361 tgtaacgata aatcctgcac ttatagaaat taacagagca ggaatctgag agaccaatcc
      421 gtcaccaata gttagtatgg tataagtaga taatgcacca gatagggaca ttccatgttg
      481 actcatacca acagaaatac ctcctattaa attgacaaaa ataataatga tgccagcaat
      541 tgcatcacct ttaatgaact tcattgctcc atcaaatgaa ccatacaatt gactctcacg
      601 ttccagaata ctgcgtcttt ctttagctcc tgcggcatca ataattccag cctttaaatc
      661 cgcatcaatg ctcatttgtt tccccggcat accgtcaaga gaaaagcgag cagctacctc
      721 cgcaaccctt tcagatcctt ttgttataac aatgaactgc acaacagtta ctattgaaaa
      781 aataacaaaa cctacagcca gactatcacc aatgacaaac tggccaaatg tagtaataat
      841 tttacccgca tccgcatcaa ctaaaattag tcgactagta cttattgata aagcgagacg
      901 aaataatgtc gttatcaaca aaaccgaagg gaatgtagaa aaactcaata ttctttcaat
      961 ataaaatgat cccata
//
LOCUS       DAZPAC010000522          973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZPAC010000522 DAZPAC010000000
VERSION     DAZPAC010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.537"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>973)
                     /locus_tag="ACHUPM_004823"
     CDS             complement(<1..>973)
                     /locus_tag="ACHUPM_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0872328.1"
                     /translation="EDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFI
                     NWMRDCHSTDDKDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKS
                     NEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFE
                     KKFPLTELNNPAHDSSAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVS
                     FRSIIAEQKKEPEMMVRYYISSADLTAEKFATAIRNHWHVENNLHWRLDVVMNEDDCK
                     IRRGNAAELFSGIRHIAINVLTNDKVFKAGLRRKMRKAAMDRNYLASV"
BASE COUNT          248 a          234 c          172 g          319 t
ORIGIN      
        1 actgacgcga gatagtttct gtccatggct gcttttcgca tcttacgtct taaccctgcc
       61 ttgaatacct tatcattcgt caaaacatta atagcaatgt gccgtatccc tgaaaataat
      121 tctgctgcat ttcctcttct tattttgcag tcgtcttcat tcattaccac gtccagacgc
      181 cagtgcagat tattctccac gtgccagtga tttcgaatcg ctgtcgcaaa cttctcagcg
      241 gttaaatcag cagaactgat ataatatctg accatcattt cgggctcttt cttttgctct
      301 gctattattg agcgaaagga gactgccacg cataattttt tcagcccttt ccattcaaac
      361 gtgaaatcaa taagttcatc agggacatcg caaacaatat gaagacggat ttcttctctg
      421 ccgtgactct tttcactcat tgcggaactg tcatgcgctg gattatttaa ttctgtcagc
      481 ggaaattttt tctcaaaggc tttatttagc cgcccctggt ttccttttac cgcaaataaa
      541 taatcacctc cctgtttttg tatcttctct gcaatatctt tctggcaacc catcgcatca
      601 gttgtgatga tttttccttt aatatccagc atgttaagaa gttctgggat agctgtaatc
      661 tcattagatt tctcatccgt cttgatctgt ccgatgacca gactgtgcat tgttgagaac
      721 gcactaatga catgaatcgc tcccctgcgg cgactcttgt cataagagtg ccggagcgtt
      781 tttccatcaa ttgcaatgac gtctttatca tctgtagaat ggcagtcacg catccagtta
      841 ataaagcact cgtgaaattt tgcaggactg atacaggata caactctggc aatggtatcg
      901 tgaacaggaa tgccattttc aaaatcacca tattgcttca aaaaatcgag atgtgtttcc
      961 ccaaaatcct cta
//
LOCUS       DAZPAC010000523          965 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZPAC010000523 DAZPAC010000000
VERSION     DAZPAC010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.538"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..297)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUPM_004824"
     CDS             complement(<1..297)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUPM_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR0872329.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPS"
     gene            complement(345..917)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUPM_004825"
     CDS             complement(345..917)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUPM_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR0872330.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          279 a          226 c          191 g          269 t
ORIGIN      
        1 cgaaggttct aatcgtgata ctggcggaat aacggtaaac ggcgctttgc ttttatcctc
       61 tttattaaat ggatctaccc aagcctggat gagataaggt attttttctt ctttgctaaa
      121 tacagaaata gatttatcag cattattact taagtaaacc acccgcgtac caccaatgac
      181 aacgcctgca taagttaatg ctggttggcc taaaaaaacg ctgagtataa tcgcggtaaa
      241 taacttacat gaaactgttg ttttaaagag cctgattaaa caactttttc tggacataac
      301 gactccctgg ccaaagccaa ctgaataaaa aggcccttga taaattattt cttatattcg
      361 acagtaaatt gtgaatcagc gttcgccgta ccagccgtta cggtcgcatt ggttgccaca
      421 tagcgtgcaa taaacttcat ttcagccttg ccttcagcat cgatagcggt ataagcagag
      481 tcgttataaa gtttaacctg cgaattatcg gactggttaa aaatacgaat accaacgccg
      541 gttgccgcaa tcgcttgtcc tgtaccggta aaatcaccag ggttgcctgc tgtaagcggc
      601 gtacccactt tcaggtcgcc gtcgccgcca ggcgcttctg taccatcaaa acgaacggct
      661 gcttgagtgt acgttgccgg gcaattttca agtttaatac tgaacttctg tggcgacgcc
      721 agactaccta cacctgaaaa tgcattttta ctgactttac ccagtgggac aatttgatcg
      781 gctgtatcgg aggtaacttc acaagtagtg tcgacaattt caccgacgaa gtgaacaaca
      841 ccatcttctg ccagtgcatg cccggccagc attagagatg ccagagtgaa tgcaccgcct
      901 ataatattac gcttcataaa aactccattg atataatata tcctttacca tgaatcatca
      961 cttat
//
LOCUS       DAZPAC010000524          960 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZPAC010000524 DAZPAC010000000
VERSION     DAZPAC010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.539"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>960
                     /locus_tag="ACHUPM_004826"
     CDS             <1..>960
                     /locus_tag="ACHUPM_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HFR0872331.1"
                     /translation="ILGYQANSNGKVIVEKGGEWLIKNNDSSIEFQIGNQGAGEATIR
                     EGGLITAENTIIGGNATGIGTLNVQDQDSVITVRRLYNGYFGNGTVNISNNGLINNKE
                     YSLVGVQDGSHGVVNVTDKGHWSFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIIT
                     AGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGENGV
                     GNVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLM
                     DATSSFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIGGNASG"
BASE COUNT          273 a          173 c          266 g          248 t
ORIGIN      
        1 cattttagga tatcaagcga acagtaatgg taaggttatc gttgaaaagg gtggcgagtg
       61 gctaataaaa aataatgatt cctcaattga atttcaaatt ggtaatcaag gagctgggga
      121 ggcgactatt cgcgagggtg ggctgattac ggctgaaaat acgattatcg gtggcaatgc
      181 caccggtatc ggaaccctga atgtgcagga tcaagactct gtcatcacgg tacgcagact
      241 ctataatggg tatttcggta atggcacagt caatatttcc aataatggtc tgattaataa
      301 caaagaatat tcattggtgg gcgttcagga cggttcccac ggtgtcgtca acgtgaccga
      361 taaagggcat tggagtttcc tcggaacagg cgaagctttc cgctatatct atatcggtga
      421 tgctggtgac ggtgaactta atgtctcgcg tgaaggtaaa gtagattcgg gaattatcac
      481 tgcggggatg aaagaaacag gcacaggcaa cattactgtt aaggataaga actccgttat
      541 cactaatctc ggaactaatc ttggttatga cggccacggc gaaatgaata tcagtaatga
      601 ggggcttgtt gtcagcaacg gaggaagctc actcggttat ggagaaaacg gcgtcgggaa
      661 tgtcagcatc accacggggg ggatgtggga ggtcaataag aatgtctata ccaccattgg
      721 tgttgcgggc gtcggaaacc tcaatattag cgatggcggt aagtttgtat cgcaaaatat
      781 tacttttttg ggcgataaag caagcggtat cggcacactg aacctgatgg atgcgacatc
      841 atcgttcgat actgtgggta tcaatgtcgg taattttggt agcggtatcg taaatgtcag
      901 taatggtgcc acccttaatt caacgggcta tggatttatc ggaggaaatg cctccggtaa
//
LOCUS       DAZPAC010000525          958 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZPAC010000525 DAZPAC010000000
VERSION     DAZPAC010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.540"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>958
                     /locus_tag="ACHUPM_004827"
     CDS             <1..>958
                     /locus_tag="ACHUPM_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR0872332.1"
                     /translation="FLDAGGLRDIEVEAVTKMLACGTRILGVKEYNCDKPECPHVRYV
                     TNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTLWPVFESNRWLLNDV
                     CRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGCLNKHGQWKKLSF
                     LKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQWRSLVLKAGGKYWHVYMSK
                     KTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKTGETATETLTQRELV
                     ARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKPE"
BASE COUNT          221 a          240 c          310 g          187 t
ORIGIN      
        1 ttcctggatg cgggcggtct gcgcgatatc gaggttgagg ctgtcacaaa aatgctggcc
       61 tgcggcacgc ggatactggg agtgaaggaa tacaactgcg ataaacctga gtgcccccac
      121 gtcagatacg tcactaactc atgcggcagc cgtgcctgcc cgtcctgcgg aaaaaaggcc
      181 acagacctgt ggatagcgac acagctgaat cgtcttcctg actgcgactg ggtacatctg
      241 gtcttcaccc tgccggacac gctgtggccg gtgttcgaaa gcaaccggtg gctgctgaat
      301 gacgtgtgcc gtctggcggt ggagaatctg ctgtatgccg cccggaaacg ggggcaggaa
      361 cccggtatct tctgcgccat ccacacgtat ggccgtcgtc tcaactggca tccgcatgta
      421 catgtgtctg taacctgtgg atgtctgaat aagcatggtc agtggaaaaa gctgagcttc
      481 ctgaaagacg cgatgcgttc gcggtggatg tggaatatgc ggcaacggct tctgaaagcg
      541 tggtcagagg ggctggcgat gccggagtcg ttgtcacata tcacgacgga atcacagtgg
      601 agaagcctgg tgctgaaagc cggaggaaaa tactggcatg tgtacatgtc gaaaaaaacg
      661 gccggcggga ggaatacggc gcgctacctg ggtcgttatc tgaagaagcc gccgatagcg
      721 gcctcccggc tggcacatta caacggaggg gcgagcctga gcttccgtta cctggaccac
      781 aaaacgggag aaacggcgac ggaaacgctg acacagcgtg agctggtcgc gaggctgaaa
      841 cagcacatcc cggagaagtt ttttaagatg gtgaggtact tcgggttcct tgccaaccgg
      901 gtgtgtggag agaagctgcc gcaggtgtac cgtgctctgg ggatggataa accggaac
//
LOCUS       DAZPAC010000526          957 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZPAC010000526 DAZPAC010000000
VERSION     DAZPAC010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.541"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..395
                     /locus_tag="ACHUPM_004828"
     CDS             <1..395
                     /locus_tag="ACHUPM_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR0872333.1"
                     /translation="INAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSLAA
                     VLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTVLD
                     GKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            569..>957
                     /locus_tag="ACHUPM_004829"
     CDS             569..>957
                     /locus_tag="ACHUPM_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR0872334.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHT"
BASE COUNT          327 a          177 c          188 g          265 t
ORIGIN      
        1 tgattaatgc cattgtcaaa ccgttctatg aacgtcagat gagtaagtca gataatccaa
       61 ttgataaagt tgtaaggtca aaggtggaac ccacagacca agcattgact ggtgacatga
      121 actctttagc cgctgtttta tgggctatgg ttcagggcaa atcaaaagat gttccacagt
      181 tttcacctga agcattaaat aatctgaaag gtcgagtgga aacattcaca caagatttaa
      241 aggttatcaa accgtcagta aatattcagc ctcagacaat caatatcaca actcagacgg
      301 tacttgatgg aaaggttatt gatgaaagaa ccacatcgca tattaatcgt atgcaggaag
      361 ataccttgat tagctcagct tatccagagg aataaacaat gttagagcta ctaatattta
      421 ttgccacaca tggcaatcta ttaatcttta tctagtatga cgattcaact atgaatagtc
      481 gaatgctacg tgaaatcttc tgaacagata ccaacatcaa aaattagatt tcacgtagct
      541 cacgcttaac agaataggaa taacaacaat gtttgacaca aagcaaatta gatactcagg
      601 taaagatggt atctattttc accttagaga taatgtggaa tcttttcttg ccttaacagc
      661 tactgaacag atggactatg actcaccgat gatggtgaca actcaggcta tgcaatcggg
      721 gcaaactatc actgataatg ttcagagagc acctaagacc atctctatta gcggtgttgt
      781 agtggttgat tcgacgggtt cagttctatt aaccagacag gggcaacttg tagaaacctt
      841 tatttctacg gttgaacaat ggcgagacca gaagcaaatc atcactattg tttgtagaga
      901 tggaatagct atcactgata gtatcattac tcagtttaaa gcgagtaaag agcatac
//
LOCUS       DAZPAC010000527          949 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZPAC010000527 DAZPAC010000000
VERSION     DAZPAC010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.542"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..949
                     /locus_tag="ACHUPM_004830"
                     /pseudo
     CDS             1..949
                     /locus_tag="ACHUPM_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          234 a          233 c          289 g          193 t
ORIGIN      
        1 cctgcctctg aacttgctgc cgccatgaag cagattaaag aactccagcg cctgctcggc
       61 aagaaaacga tggaaaatga actcctcaaa gaagccgttg aatatggacg ggcaaaaaag
      121 tggatagcgc acgcgccctt attgcccggg gatggggagt aagcttagtc agccgttgtc
      181 tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg aaccgatgac tggatggatg
      241 gccgccgcag tcgtcacact gatgatacgg atgtgcttct ccgtatacac catgttatcg
      301 gagagctgcc cacgtatggt tatcgtcggg tatgggcgct gcttcgcaga caggcagaac
      361 ttgatggtat gcctgcgatc aatgccaaac gtgtttaccg gatcatgcgc cagaatgcgc
      421 tgttgcttga gcgaaaacct gctgtaccgc catcgaaacg ggcacataca ggcagagtgg
      481 ccgtgaaaga aagcaatcag cgatggtgct ctgacgggtt cgagttctgc tgtgataacg
      541 gagagagact gcgtgtcacg ttcgcgctgg actgctgtga tcgtgaggca ctgcactggg
      601 cggtgactac cggcggcttc aacagtgaaa cagtacagga cgtcatgctg ggagcggtgg
      661 aacgccgctt cggcaacgat cttccgtcgt ctccagtgga gtggctgacg gataatggtt
      721 catgctaccg ggctaatgaa acacgccagt tcgcccggat gttgggactt gaaccgaaga
      781 acacggcggt gcggagtccg gagagtaacg gaatagcaga gagcttcgtg aaaacgataa
      841 agcgtgacta catcagtatc atgcccaaac cagacgggtt aacggcagca aagaaccttg
      901 cagaggcgtt cgagcattat aacgaatggc atccgcatag tgcgctggg
//
LOCUS       DAZPAC010000528          940 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZPAC010000528 DAZPAC010000000
VERSION     DAZPAC010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.543"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            66..533
                     /gene="ipgC"
                     /locus_tag="ACHUPM_004831"
     CDS             66..533
                     /gene="ipgC"
                     /locus_tag="ACHUPM_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFR0872335.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            539..>940
                     /locus_tag="ACHUPM_004832"
     CDS             539..>940
                     /locus_tag="ACHUPM_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin"
                     /protein_id="HFR0872336.1"
                     /translation="MHNVSTTTTGLSLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANKNINTTNSHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLT"
BASE COUNT          349 a          179 c          140 g          272 t
ORIGIN      
        1 caaattaaat ctgtttatat taaatcttat acttattaaa gtaaagcaga caaaaaggag
       61 accttatgtc tttaaatatc actgaaaatg aaagcatctc tactgcagta attgatgcaa
      121 ttaactctgg cgctacactg aaagatatta atgcaattcc tgatgatatg atggatgaca
      181 tttattcata tgcttatgac ttttacaaca aaggaagaat agaggaagct gaagttttct
      241 tcagattttt atgtatatac gacttttaca atgtagacta cattatggga ctcgcagcta
      301 tttatcagat aaaagaacag ttccaacaag cagcagacct ttatgctgtc gcttttgcat
      361 taggaaaaaa tgactataca ccggtattcc atactggaca atgccagctt cggttgaaag
      421 cccccttgaa agccaaagag tgcttcgaac tcgtaattca acacagcaat gatgaaaaat
      481 taaaaataaa agcacaatca tacttggacg caattcagga tatcaaggag taattattat
      541 gcataatgta agtaccacaa ccactggttt gtctcttgcc aaaatattgg cttccactga
      601 gcttggagac aatactatcc aggctgcaaa tgatgcagct aacaaattat tttctcttac
      661 aattgctgat cttactgcta acaaaaatat taatacaact aattcacact caacttcaaa
      721 tatattaatc cctgaactta aagcaccaaa gtcattaaat gcaagttccc aactaacgct
      781 tttaattgga aaccttattc aaatactcgg tgaaaaatct ttaactgcat taacaaataa
      841 aattactgct tggaagtctc agcaacaggc aagacagcaa aaaaacctag aattctccga
      901 taaaattaat actcttctat ctgaaactga aggactaacc
//
LOCUS       DAZPAC010000529          933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZPAC010000529 DAZPAC010000000
VERSION     DAZPAC010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.544"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(195..>933)
                     /locus_tag="ACHUPM_004833"
     CDS             complement(195..>933)
                     /locus_tag="ACHUPM_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0872337.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          257 t
ORIGIN      
        1 gcttctccga taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg
       61 ttgaccgagc cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt
      121 atcctgtcta tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta
      181 ctgctggcat ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct
      241 aatataaccg tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc
      301 acttccgtca ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc
      361 attccttttg tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc
      421 ggaataccga caacggcgct taatgggatg gcgaagggat tatctgctcc tgcatgagcg
      481 gcaatccacg ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa
      541 agatatggca gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga
      601 ttaatactta ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta
      661 gccgctggac tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt
      721 gctggagcac aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc
      781 agagaatcca ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac
      841 aaggtgactt tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat
      901 ggtgaaacaa ataaaaaagt gagagtcgga cca
//
LOCUS       DAZPAC010000530          931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZPAC010000530 DAZPAC010000000
VERSION     DAZPAC010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.545"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(1..177)
                     /locus_tag="ACHUPM_004834"
                     /pseudo
     CDS             complement(1..177)
                     /locus_tag="ACHUPM_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            161..419
                     /locus_tag="ACHUPM_004835"
                     /pseudo
     CDS             161..419
                     /locus_tag="ACHUPM_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(528..>931)
                     /locus_tag="ACHUPM_004836"
     CDS             complement(528..>931)
                     /locus_tag="ACHUPM_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872338.1"
                     /translation="KKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGFV
                     SFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTGIIFYML
                     DEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
BASE COUNT          271 a          213 c          169 g          278 t
ORIGIN      
        1 gctgtgctgc cacaggaagc gagttccctt tgcctgacga cgactttcaa caggaagatg
       61 cggatgttca ttctggcggg ttttatccac cacctcaccc ggttgtttcg ccacatgaaa
      121 gcgatcgaag gctattttct caaccgcatt gggtaagtgg atgcgcgctg ctcttagttt
      181 gctgagccag tttagcattg ccttctacat gaccgatgcc tgagccggta taccgaactt
      241 tattgtcctc aaccagtcat gttataagca agaaatgcac tcagcggata gagcgacata
      301 atctgaacct gcggacacat cttaagcggc ttacccgcaa aaccatatgc ttctcaaagt
      361 ctgatgatat gcactacaag atcatcggct ggtatctgac aatcaaccac caccactaaa
      421 tctgcgtcac gacctgcaac tgagtctggt tgaacaggct gaggccctgc tgaagcaacg
      481 cgaacctctc gactcttgtt ggattttacc aaatagtttt tctaaaatta aacacattta
      541 tataagttct ttgctgaaca aaacgcagcc cgccatcctt tttcattcat aaattcagca
      601 atggcagata aaactttttc atcaagcata taaaaaataa tgccagtaga ctcaatatgc
      661 ctcatacaaa taaggccgca catatccgta atatatttgt caatgctgct ataatttgca
      721 tggtaatatg ccatgctatt atctgatgat tttacatatg cagtatgaca tatttttcct
      781 tcacgattaa caaaactgac aaaaccactt tcaccaggag taatatataa agatgaattt
      841 aacgtgcgct cactaatatt cattaattgt tgaaattcaa tggaatagtt ttcagttaat
      901 gtatctatta tcttggcttt gtctttcttt g
//
LOCUS       DAZPAC010000531          912 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZPAC010000531 DAZPAC010000000
VERSION     DAZPAC010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.546"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(29..829)
                     /locus_tag="ACHUPM_004837"
                     /pseudo
     CDS             complement(29..829)
                     /locus_tag="ACHUPM_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          259 a          217 c          174 g          262 t
ORIGIN      
        1 actaaatcag taagttggca gcatcaccca ttgcaagata aacattgttg gcgtcgtatt
       61 ttgcaccaaa tgtccatgct tcagctgtat caccaccagc aaatttgctg tattcattgt
      121 agtgataccc gccaagagct acttgagaat tggtacggtc agagtttgag taagcggcac
      181 ctaagctcaa accgaagtca aagtcatatg aagtggaaag accaaaacca tcaccgttct
      241 ggaagcgaac gtcttcgtga ccatttttag taccttcttg atcttctcct gcattgctgc
      301 cttcgttgtt accttgatac tgcaatgcaa agttcagacc ttccaccaga ccgaagaaat
      361 cactattgcg gtaagtagct accccatttg cacggcctgc catgaagtta tcagtccagg
      421 tataggagtc gccgccgaat acaggaagca tatctgtcca ggcttcaacg tcgtaaatca
      481 caccatagtt acgaccatag tcaaaggagc ccatctcgcc gaacttcaga cctgcaaaag
      541 caagtttgac ataagtatta tcagaacctt cagtgtcgct agcctttgtt ttggtcagtg
      601 cttgaccata accagttaac tgatctgtaa tttgggtctc acctttgaag cctaactcga
      661 tgatagtttc atcgccgtca tcgccagggt tatcggaaaa agtatgacga gcatcaactt
      721 ttccgtacag atccagttta ttgccatttt tattataaat ttcagccgca tttgctgcgc
      781 cagcaactaa taacgctggg accagcattg ccagaacttt tcttttcatt atgtattccc
      841 ttgtgattat aatcttcatg aatatatcaa taagtgccgt tatccaaaaa aaaagcacat
      901 ttggatacta tt
//
LOCUS       DAZPAC010000532          904 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZPAC010000532 DAZPAC010000000
VERSION     DAZPAC010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.547"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            59..>904
                     /locus_tag="ACHUPM_004838"
     CDS             59..>904
                     /locus_tag="ACHUPM_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0872339.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRD"
BASE COUNT          318 a          138 c          152 g          296 t
ORIGIN      
        1 ctttcagaca tgtattgttt attttgttac taacaagtaa aaatattaca ggttatttat
       61 gaaagtattt cacaatatat ttaagcactt ctcttcaaac catcaagata agcattcaga
      121 taaagttaac agtcatcaac accatggcaa ggttgataaa aaatatcgat caaacacagt
      181 tgaagttaat atgctgaatg acttttcgca aatagataac gatttaggcc tgaatatcat
      241 ctatttcctg caacatggtc actggagggt aaatgaacat agtcttcaga tggagaaaat
      301 atggttttac aatagtgagc catctataga tattcaagaa tataatagtt tcgccgataa
      361 tactactaac acgtttatct ttacaattat accagacaat aaccatgtga taaaattatc
      421 gtctcccatt acagttaaga ttgagtgtaa ggatggttat tattttatta atttctccgg
      481 agataaatca gacataattt ataaatcaga cataacttat aaagttaatg ggctttctat
      541 catacctgaa aatttcttta ctcttcttag cggtaatttt aaagctgact ggcgttggga
      601 tgtctctaaa gagacgtttc ctaaggataa gtttgatagc tatgttaaat ctgtgttttc
      661 taaaatagac ttttataaac aatgtggtgt gattaaccca caaaatgcca acacggctta
      721 ttttggcgat acagatggaa gagtaggcgc tgtactttat gcattgctcg tttcagggca
      781 tataggcatc agggaaaagg gttggagttt attatgtgaa ttattaaaac acgaaaacat
      841 ggcttcatct gcatacatac ataaaaataa taaattgctc tacaatttat taaatacgag
      901 agac
//
LOCUS       DAZPAC010000533          900 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZPAC010000533 DAZPAC010000000
VERSION     DAZPAC010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.548"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            42..233
                     /locus_tag="ACHUPM_004839"
     CDS             42..233
                     /locus_tag="ACHUPM_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872340.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTAACRLT
                     CRWRGIRIIQAAFNAFAKV"
     gene            306..557
                     /locus_tag="ACHUPM_004840"
     CDS             306..557
                     /locus_tag="ACHUPM_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFR0872341.1"
                     /translation="MPNIILSETSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            554..841
                     /locus_tag="ACHUPM_004841"
     CDS             554..841
                     /locus_tag="ACHUPM_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0872342.1"
                     /translation="MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
BASE COUNT          262 a          184 c          223 g          231 t
ORIGIN      
        1 ccctcgtcgg gaccactccg accgcgcaca gaagctctgt catggctgaa aacgggtatg
       61 gcttagcagg gctggggatg ggtaaggtga aatctgtcaa tcagtaccgg ctaacgccgg
      121 gcttcggcgg ttttactccg gtatcacatg taaccgcagc gtgccgcctt acatgccgct
      181 ggcgcggcat cagaataata caagcagcat ttaatgcatt tgcaaaagtg tgagtaataa
      241 agacttgtta agtggtcgtt tcagggatat cgttaagact tgttaaacag tctcaaaggt
      301 gcgttatgcc taacatcatt ctgtctgaga ccagtgcaag tgtcagtgaa ctgaaaaaaa
      361 atcctatggc tacagtcagt gccggagatg gttaccctgt cgccattctt aaccggaatc
      421 agccggcatt ctactgtgtg cctgctgaac tgtatgaaag gatgcttgat gctctggatg
      481 accaggaact ggtgaaactg gttactgaac gcagcaatca gccactgcat gatgtggacc
      541 tggacagtta tttatgactt atacggtaaa attcagggat gatgcgctca aggaatggct
      601 gaaactggat aagaccattc aacaacagtt tgtgaaaaaa ctaaaaaaat gcagcgaaaa
      661 tcctcatata ccgtcggcaa aactaagagg gctaaaggac tgctacaaaa ttaaactacg
      721 tgcatcggga tttcgtctgg tttatcaggt gattgacgat atgttaatta ttgcggttgt
      781 tgcggtagga aaacgagaac gtagtaacgt ttataatctc gccagtgaga gaatgagata
      841 gtaacatata aataaccttt cccgcagata actcagaatt gttattttct ctgtggactc
//
LOCUS       DAZPAC010000534          879 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZPAC010000534 DAZPAC010000000
VERSION     DAZPAC010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.549"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            108..635
                     /locus_tag="ACHUPM_004842"
     CDS             108..635
                     /locus_tag="ACHUPM_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR0872343.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            693..>879
                     /locus_tag="ACHUPM_004843"
     CDS             693..>879
                     /locus_tag="ACHUPM_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFR0872344.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCA"
BASE COUNT          201 a          233 c          282 g          163 t
ORIGIN      
        1 ccctgtgaag tggacggcag gaggagcttt cgctcctctg aaggggcaca tgaacgtccc
       61 gtcgccggta tccggtttat ctcacatctt tcagtcagga ggttcttatg agtgcacgtg
      121 gtatcaacaa ggtcatcctc gtcgggcgtc tgggcaatga tccggaagtc cgttacatcc
      181 ccaacggggg cgcagtggca aacctgcagg tggccacatc agaaagctgg cgtgacaaac
      241 agacggggga gatgcgggag cagacggaat ggcaccgcgt ggtgctgttc ggcaagctcg
      301 cggaagtggc aggtgaatat ctgcgcaagg gtgcgcaggt ctacatcgaa ggtcagcttc
      361 gcacccgtag ctgggaagat aacggtatca cccgttacgt cactgaaatt cttgttaaga
      421 ccacgggcac cgtgcagatg ctgggacgtg caccacagca gaacgctcag gcgcaaccga
      481 agcctcagca gaatgggcag ccacagagtg ctgacgcgac gaaaaaaggt ggcgcgaaaa
      541 cgaaaggccg tggacgtaag gccgcgcagc cagagcctca gccgcaaccg ccggaggggg
      601 aggattacgg gttttcagac gacatcccgt tctgatcggg ctgactgtga caaccgcccc
      661 gccctgtgcg gggcatcacc ggagatatga ggatgagcga atatttcaga atacttcagg
      721 gactgccgga cggctccttt acccgcgaac aggcggaagc cgttgccgca cagtaccgga
      781 acgtctttat cgaggatgat cagggaacgc attttcgcct ggttgtccgt caggatggca
      841 cgttgatctg gcgctcctgg aattttgagg actgtgccg
//
LOCUS       DAZPAC010000535          877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZPAC010000535 DAZPAC010000000
VERSION     DAZPAC010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.550"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>877)
                     /locus_tag="ACHUPM_004844"
     CDS             complement(<1..>877)
                     /locus_tag="ACHUPM_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0872345.1"
                     /translation="DLLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID
                     DLNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPY
                     INDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLF
                     FNLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSI
                     LVNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPG
                     YVVKIFEEKMGNELKI"
BASE COUNT          296 a          154 c          108 g          319 t
ORIGIN      
        1 taatttttaa ttcattaccc atcttttctt caaaaatctt aacgacataa cccggaagtt
       61 tatttacaat ttcttttgtt ttttcttcat caccataaac atatcctaac tcattaagat
      121 acagctgttc gccctcataa cacttaagta attgatagaa gtaccactta gcaaaatcag
      181 gttgcttcat gaaaaaatcc ttcaaaaaaa tatcatttac taaaatgctg acaggcactt
      241 cattaaatag taacttaaca tcatcaaata accttagtat taagcgtgat ttttcttgat
      301 tgcttaagct aaaatcattg acatatgcaa ccaacttatc ccaaatcaat tcctgtaatt
      361 tacggctatt tttatagggt tctttatcga gattgaaaaa taaggtctca gctattttaa
      421 ttttagattg atcgcagcta aaatctttag attgatcgca gctaaaatca atatgattta
      481 tcacggagca tgcatacttt atcttttcag aatcaccaaa atattgcatt cttttattaa
      541 aacaccaatc cagtattctt acatcattaa tatatgggga ttttaaaaaa gtctccagaa
      601 tcgaatcgat gaccggtgta aggttaactt ttgcatcatc taaggaatca ataagaatat
      661 gagacaggtt aactttgatg tcattaattt cattgtcagt agctttctta ttattaagaa
      721 gataattatt tactgcgtta aggtcatcta tttctttcag tatccctttt tcaccaagca
      781 agtatgaaat cgattcatta catacataag gcattaatag cagatgttct ttccttattg
      841 ctaataatgc attgttcaat ggtttttcta ataaatc
//
LOCUS       DAZPAC010000536          876 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZPAC010000536 DAZPAC010000000
VERSION     DAZPAC010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.551"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHUPM_004845"
     CDS             complement(<1..304)
                     /locus_tag="ACHUPM_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR0872346.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          275 a          200 c          186 g          215 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
      181 tgaccagaga gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
      301 acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg
      361 ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat
      421 ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa
      481 caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat
      541 tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt
      601 attcacaaca ataaataatt caataacaat cattgaaatg aatgatggtt tgtataagga
      661 atatacgcta ccgcccaccc tgataacatg atctgcacat tgccatctta acgtcgttaa
      721 acactggctt aatagtggat atttcaaatt aggaatttat taaattcacc tgatgaatat
      781 aataagcaaa tagcatagaa aataatggca actccccccg agaggaccat ttccaaagca
      841 ctgcaccacc acctggtgcc cggatgatct tgcttc
//
LOCUS       DAZPAC010000537          873 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZPAC010000537 DAZPAC010000000
VERSION     DAZPAC010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.552"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>873
                     /gene="cadA"
                     /locus_tag="ACHUPM_004846"
     CDS             <1..>873
                     /gene="cadA"
                     /locus_tag="ACHUPM_004846"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR0872347.1"
                     /translation="FSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKG
                     DVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIK
                     RLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGM
                     EKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDF
                     KRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLP
                     TMVMTPYAAFQKEL"
BASE COUNT          236 a          219 c          212 g          206 t
ORIGIN      
        1 acttctcacc gatttacgaa ggtaaatgcg gtatgagcgg tggccgtgta gaagggaaag
       61 tgatttacga aacccagtcc actcacaaac tgctggcggc gttctctcag gcttccatga
      121 tccacgttaa aggtgacgta aacgaagaaa cctttaacga agcctacatg atgcacacca
      181 ccacttctcc tcactacggt atcgtggcgt ccactgaaac cgctgcggcg atgatgaaag
      241 gcaatgcagg taaacgtttg atcaacggtt ccattgaacg tgcgatcaaa ttccgtaaag
      301 agatcaaacg tctgagaacg gaatctgatg gctggttctt tgatgtatgg cagccggatc
      361 atatcgatac gactgaatgc tggccgctgc gttctgacag cacctggcac ggcttcaaaa
      421 acatcgataa cgagcacatg tatcttgacc cgatcaaagt caccctgctg actccgggga
      481 tggaaaaaga cggcaccatg agcaactttg gtattccggc cagcatcgtg gcgaaatacc
      541 tcgacgaaca tggcatcgtt gttgagaaaa ccggtccgta taacctgctg ttcctgttca
      601 gcatcggtat cgataagacc aaagcactga gcctgctgcg tgctctgact gacttcaaac
      661 gtgcgttcga cctgaacctg cgtgtgaaaa acatgctgcc gtctctgtat cgtgaagatc
      721 ctgaattcta tgaaaacatg cgtattcagg aactggctca gaatatccac aaactgattg
      781 ttcaccacaa tctgccggat ctgatgtatc gcgcatttga agtgctgccg actatggtaa
      841 tgactccgta tgctgcgttc cagaaagagc tgc
//
LOCUS       DAZPAC010000538          857 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZPAC010000538 DAZPAC010000000
VERSION     DAZPAC010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.553"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..570
                     /locus_tag="ACHUPM_004847"
     CDS             <1..570
                     /locus_tag="ACHUPM_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0872348.1"
                     /translation="LQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAV
                     SKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQ
                     LWRGGQVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAA
                     GHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(610..>857)
                     /locus_tag="ACHUPM_004848"
     CDS             complement(610..>857)
                     /locus_tag="ACHUPM_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFR0872349.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
BASE COUNT          236 a          240 c          221 g          160 t
ORIGIN      
        1 cttcagcgac agttaagccg caaggtcaaa ttcagcaaca actggcagaa gcagaaacgc
       61 aaaatacagc gactgcattc ctgtatcgca aatatccgca gggactacct tcacaaagtc
      121 acaacggccg tcagcaaaaa ccacgcaatg atagtcattg aggatttgaa ggtcagcaac
      181 atgtcaaagt cagcagcggg tacggtcagc cagccggggc gcaatgtccg ggcaaaatca
      241 ggtttaaacc gttcgatact ggatcagggc tggtatgaaa tgcaccgcca gcttgagtac
      301 aagcagctct ggcgtggcgg tcaggtgctt gctgttccgc cagcgtatac aagccagcgt
      361 tgcgcgtact gtggtcatac agcgaaagag aaccgcctgt cacaaagtaa attcagatgc
      421 caggtatgtg gatatacagc gaacgccgat gtaaatggtg ctcgtaacat tttagcggcg
      481 gggcacgccg ttcttgcctg tggagagatg gtgcagtcag gccgcccgtt gaagcaggaa
      541 cccaccgaaa tgattcaggc gacagcctga acgtagcagg gatccacgtc cttcagggcg
      601 tggaggatgt caatatcggt ttctgtcagc gattaaattc aacaccacgt aacaaacgcc
      661 tccggcaatt aatccccaga acgcagaacc aatcccgacc agcgtcaatc cacttgccgt
      721 taccagaaat gccaccaccg ccgcgtcgcg ctcacgctca cgctcattat gcagcgcctg
      781 atacaaactg ccgccaatgg tactcaacag cgccagacct gccagcatct ggatccaact
      841 tacgggcagg gcagcca
//
LOCUS       DAZPAC010000539          829 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZPAC010000539 DAZPAC010000000
VERSION     DAZPAC010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.554"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(42..812)
                     /locus_tag="ACHUPM_004849"
     CDS             complement(42..812)
                     /locus_tag="ACHUPM_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872350.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPNHPGWISIYVR
                     LDAPRLATLLINRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLAEDEIRAVLAAVRDAIRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSAVEMYLVSECVEHILSSGLACDVLR
                     IPDEPPRRWFDRDVLREVVREARAEIRSMADALAKIRG"
BASE COUNT          161 a          272 c          238 g          158 t
ORIGIN      
        1 cggggactgg caggaaaagg cggggatttt ccccgccatc atcagccccg gatttttgcg
       61 agggcatccg ccatgctgcg gatttcggcc cgggcctccc ggaccacctc ccgcagaaca
      121 tcacggtcaa accagcggcg gggcggttca tccggtatac gcagcacatc gcaggccagc
      181 ccactggaca ggatatgctc cacacactca ctgaccaggt acatttccac tgccgaaaaa
      241 cgtgctcccc ggttcacgat ggcatcgagc accacgggca gattttcgtc gtcttcatcg
      301 agccagcagg ggtgatcgct ttccttcaca accagacgaa agcggcgcgt ctggcgggtc
      361 agcaacgtct gaccggtggt ggtcagcgcc gcccggatac gccgcgcatc gtctgccacc
      421 tggtaacaga tgcttcgtat tgcatcgcgc accgccgcaa gtacagccct gatttcgtct
      481 tctgccagcc actcaccggc atacgggaaa gacacctgcg ttccgtctgc cggaagtacc
      541 ggcagcgact gccactgact gccggacaga acaagttccg ccccggttcc ggtcagtttc
      601 tgaatggcag aggccaggtg cggcggcagt acgccgccgt gacggttgat aagtaacgtc
      661 gccagccggg gcgcatccag ccgcacgtaa atgctgatcc agcccggatg gttcggattt
      721 cgctggtcaa catccgtctc ttccggccag ccctgcgcca gcagcaggtt acgggcgtgg
      781 cgacaaatca gtgcattcag cgtttcgttc atgattcatg gtctccctg
//
LOCUS       DAZPAC010000540          822 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZPAC010000540 DAZPAC010000000
VERSION     DAZPAC010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.555"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            135..725
                     /locus_tag="ACHUPM_004850"
     CDS             135..725
                     /locus_tag="ACHUPM_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872351.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDLERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQVNESVTP"
BASE COUNT          172 a          233 c          271 g          146 t
ORIGIN      
        1 ggagccgtgc tgatcttctg gcatgaactg gccataaatg gctatgcctc aatgaatgcc
       61 ataaagagac aggagctcat tgacgccgac tttcagcgcc tcagaaacct catctggccg
      121 gaggatgaca ggtaatgacc cagagccgcc gaccgtcgcc actgcagcgg agagtgctga
      181 ttgtgctggc tgcccttgat gaaaaacggc cgggacccgt gctgacgcgg gacctggaac
      241 gggtgctgga gcgaagcggg gaagccccgg tctacgggcc aaacctgcgc gcctcctgcc
      301 gtcgtctgga ggatgccggc tggctgcgta ccctgcgcgc tccgaacctg cagctggcag
      361 tggagctgac ggacgccggc cgggccgtgg cacagcctct gctgctggca gaacaggacc
      421 gcattcgggc agaacagcgt gcggcagaag tcgtcgtact gccgctggtc ccggcagcag
      481 gtctgcccgc agacggcact tctgccactg atctggcggt ggagctgaac ggcataacgt
      541 atcaggcctg ccgtggggat tttgtggtac gtctcgacgg cagcacctgt ctgcagttat
      601 ggaataaaga aggcagggtg gtacgccggg agggcgatcc gctggaagtg gcgcagtggc
      661 tgcaggcctg tcacgatgcc ggaatggagg ttcgtgtaca ggttaatgaa agcgtcaccc
      721 cgtaaaaaca taccagcacg gtttacacgt tatcccatgg tggcagccgg ttaactttcc
      781 tgcgccacca gtggaaagag gccatgagca gtccgaacca aa
//
LOCUS       DAZPAC010000541          815 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZPAC010000541 DAZPAC010000000
VERSION     DAZPAC010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.556"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            142..>815
                     /locus_tag="ACHUPM_004851"
     CDS             142..>815
                     /locus_tag="ACHUPM_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR0872352.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYA"
BASE COUNT          272 a          140 c          170 g          233 t
ORIGIN      
        1 ggaaagatca tcggaagtac actttttgta ctaaataatt cgcgctaaat gttcaataat
       61 tgagatattc cttattacct aaaactattt ttcatagctt atatatgatc aaatactcct
      121 tgcataaata aggagaacaa aatggaactt aaaaaactga tggaacatat ttctattatc
      181 cccgattaca gacaagcctg gaaagtagaa cataaattgt cagatattct actgttgact
      241 atttgtgccg tcatttctgg tgcagagggt tgggaagata tagaggattt tggtgaaaca
      301 catctcgatt ttttgaagca atatggtgat tttgaaaatg gtattcctgt tcacgatact
      361 attgccagag ttgtatcctg tatcagtcct gcaaaatttc acgagtgctt tattaactgg
      421 atgcgtgact gccattcttc agatgataaa gacgtcattg caattgatgg aaaaacgctc
      481 cggcactctt atgacaagag tcgccgcaag ggagcgattc atgtcattag tgcgttctca
      541 acaatgcaca gtctggttct cgggcagatt aagacggatg agaaatccaa tgagattaca
      601 gccattcctg aacttcttaa catgctggat attaaaggaa aaatcatcac aactgatgcg
      661 atgggatgcc agaaagatat tgcagagaag atacaaaaac agggcggtga ttatttattc
      721 gcggtaaaag gaaaccaggg gcggcttaat aaagcctttg aggaaaaatt tccgctgaaa
      781 gaattaaata atccagagca tgacagttac gcaat
//
LOCUS       DAZPAC010000542          814 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZPAC010000542 DAZPAC010000000
VERSION     DAZPAC010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.557"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(246..>814)
                     /locus_tag="ACHUPM_004852"
     CDS             complement(246..>814)
                     /locus_tag="ACHUPM_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0872353.1"
                     /translation="FPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVI
                     AGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFI
                     AVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQI
                     HLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          258 a          203 c          174 g          179 t
ORIGIN      
        1 agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa
       61 atcagtaagt tggcagcatc acccgctttg gcttgataac cggataacaa cgtgcgcttt
      121 gttgatgccg gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc
      181 attacattaa gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt
      241 ccacgtcagg ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg
      301 ggcggtactt tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca
      361 ttgaggcgat agaaacggaa gtagagaatc agcgttacca tgaaaaacag agttggtaga
      421 gcaatcataa taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca
      481 tagccaatca tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc
      541 accaccatag tctgcacgga gtaagcgata ctttcacagc gtacgtgcag tttatattca
      601 ccgtaatcaa cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc
      661 acattcagca aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc
      721 attaacagga gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg
      781 cgtcgggata atgatttaac taagcgtggg aaga
//
LOCUS       DAZPAC010000543          812 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZPAC010000543 DAZPAC010000000
VERSION     DAZPAC010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.558"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..280
                     /locus_tag="ACHUPM_004853"
     CDS             <1..280
                     /locus_tag="ACHUPM_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shufflon protein D'"
                     /protein_id="HFR0872354.1"
                     /translation="KSNSGSTVITGRIANGQQIPLPTGFSASQCSWSVSNAENPQGWK
                     PNYFAGSVATYDANRIVKCGFYDEYNFHKGTFRADLTGKCSYVVACQN"
BASE COUNT          234 a          168 c          187 g          223 t
ORIGIN      
        1 taaaagcaat tctggtagta cggtgataac tggtcgtata gcaaatggtc agcaaatacc
       61 gctgccaaca ggattttctg ccagtcagtg ctcatggagt gtgagcaatg cagaaaaccc
      121 tcaaggttgg aagccgaatt actttgcagg atcagttgca acttatgatg caaatcgaat
      181 tgttaaatgt ggtttttatg atgaatacaa tttccataaa gggacattta gagctgattt
      241 aaccggaaaa tgtagttatg tcgtcgcgtg tcagaactga gattaaattg ctggcgctgc
      301 tggcctacgt gccaatccgg tacctggaaa tcatcatcag cgtcgatatg gacgaatata
      361 aaaacattta cgttatatcc caaaaacaca caggtcttag ggaggtttaa actgtgtatt
      421 aacacctatc gtattgacgg gcgtgaaatg gcagaaacag aagtcgttcc tattgacatg
      481 cctgactcta acggcgagat gacttggcaa gccaaaaact acacacagta ttcgtcctac
      541 tttatgaaga ttacttgctt gaaatagctt attggcaaat ggcatataca gttattgaac
      601 cattctgatc ttcggttgta tcgcacgcag ccgaccatga gttattggct gatgggaatg
      661 acctggtaag ccatatgtaa ccagttcgtg aggtgcatat tccgccgcca cctgtaacac
      721 gttttccagt tggacaggta gctgttgctc caggaaagcg ccaacctgtg gcctgtgctg
      781 tagcaatttg aagctcacca gatcctacct tt
//
LOCUS       DAZPAC010000544          798 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZPAC010000544 DAZPAC010000000
VERSION     DAZPAC010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.559"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            128..298
                     /locus_tag="ACHUPM_004854"
     CDS             128..298
                     /locus_tag="ACHUPM_004854"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0872355.1"
                     /translation="MTHHLGCEKNQLRSGSNSRNGCLTKIITTGDEPLEIRTLRDRNG
                     TFEPQQLKKNQP"
     gene            377..514
                     /locus_tag="ACHUPM_004855"
                     /pseudo
     CDS             377..514
                     /locus_tag="ACHUPM_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          244 a          159 c          186 g          209 t
ORIGIN      
        1 atgaaaaaca gttgacatct aataagttgc gggctttagc taatgaacta gccaaaagtc
       61 tcaaaaatcc tgaagatctc agtcagtttg attggatgct gaaaatgaaa ccgtactcaa
      121 tgctaatatg actcatcatc ttgggtgcga gaaaaatcag cttagatctg gcagcaattc
      181 ccgcaatggt tgcttaacga agattatcac tactggagat gaacctctgg aaatccgtac
      241 tctgcgtgat cgtaatggca ctttcgaacc gcaacagctg aagaaaaacc agccctagat
      301 tactaaatct gtcgggcgat ggattaactg gttcaggctg tctgatgctg aaggcctgaa
      361 gtcactgctg tcagtgatgt ggccggctct tcatgagaca ataacccgta atcatcagtg
      421 ccgttcaata tggccactgt tgaaaaaagt tcgtcacttt atggagacgg ttagcccact
      481 tcccggagaa aaacatagtc tggccaaagt gtagcggtat taggctcaca tatttagatg
      541 tagataagcc ggagctggca gttaaatcta ggcagcatca cagaaatgga caacgcccct
      601 taaggactgg cgtatggcaa tgagctgctt tattatcaag tttggtgatc gccttgacgg
      661 tcacttctga gtgaaggcgc ttgcacagaa tggtaaacgg actcgattat tgaatcttag
      721 ggataagata tatctacgat atgtcaatat acgaaatcga aagaatttat tggtgaatat
      781 agccattcaa tgtggcaa
//
LOCUS       DAZPAC010000545          795 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZPAC010000545 DAZPAC010000000
VERSION     DAZPAC010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.560"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..741)
                     /locus_tag="ACHUPM_004856"
     CDS             complement(<1..741)
                     /locus_tag="ACHUPM_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFR0872356.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQLAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          266 a          137 c          161 g          231 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaagttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattt
//
LOCUS       DAZPAC010000546          794 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZPAC010000546 DAZPAC010000000
VERSION     DAZPAC010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.561"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..80)
                     /locus_tag="ACHUPM_004857"
     CDS             complement(<1..80)
                     /locus_tag="ACHUPM_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872357.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNAS"
     gene            complement(90..>794)
                     /locus_tag="ACHUPM_004858"
     CDS             complement(90..>794)
                     /locus_tag="ACHUPM_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872358.1"
                     /translation="ITSFDYIHFITTHPSGIKDTLAWLVNASKLMTEFDDNGKIIFNI
                     NALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGALSES
                     IKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLASFA
                     CVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELDKWKR
                     ENMTPFMYVLNTPPVI"
BASE COUNT          260 a          156 c          133 g          245 t
ORIGIN      
        1 gaagcattga atgcagggaa aaataatatg gccataaaaa acatcgaaag aaactctttt
       61 aatttaacat gtaaacgcat ggttaatcct catatcacgg gtggagtgtt aagaacatac
      121 ataaatggag tcatgttttc ccttttccat ttatcaagtt cctgttgccg ttttagtcca
      181 tctctaattg catattttaa tttttctgat aaatggcatt gagcatcgat ttcatttaaa
      241 acaactgtac acactatccc tgtcagtaaa attgctcctc cgacaagaat tactggaaag
      301 ccaaagctaa cacaggcaaa agaagccagg cttccaatag cggttgcaac accaccggct
      361 ataactgctt tggccacatc cattgaaaaa tcgccaagaa aattaaccag atctcgctct
      421 gaactcataa taaattctat ggttctccag gctactgaaa accagataca gaacttaact
      481 ccttttattg attcagataa agctcctcgc cagccaatag cgagttccag aacttgaggc
      541 gctcgtgaag agaaaatata tttaccgagc ttttctaaaa gccaggggct ggatgttgtg
      601 atttttatat atttctctcc aagtatttca aagtaacttg cttttgtata ctttaaggca
      661 ttaatgttaa atataatttt cccattgtca tcaaactcag tcattaattt actcgcgtta
      721 actaaccagg cgagtgtatc ttttatacct gaaggatgtg tagtgataaa atgaatgtaa
      781 tcaaatgatg ttat
//
LOCUS       DAZPAC010000547          790 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZPAC010000547 DAZPAC010000000
VERSION     DAZPAC010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.563"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            52..790
                     /locus_tag="ACHUPM_004859"
                     /pseudo
     CDS             52..790
                     /locus_tag="ACHUPM_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          228 a          194 c          208 g          160 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt
//
LOCUS       DAZPAC010000548          788 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZPAC010000548 DAZPAC010000000
VERSION     DAZPAC010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.564"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            211..>788
                     /locus_tag="ACHUPM_004860"
     CDS             211..>788
                     /locus_tag="ACHUPM_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HFR0872359.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSP"
BASE COUNT          172 a          237 c          205 g          174 t
ORIGIN      
        1 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
       61 atccgggggg tgatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
      121 tcaggcactt ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta
      181 tctggttaac ccatatacaa gggagacaga atgaccgaat ccagcgatta cgaatccgtc
      241 caggtcttta tcggcgttga tgtcggtaaa gatacgcatc acgctgttgc cattaatcgt
      301 tcaggtaaac gcctgttcga taaagcatta cccaacgacg aaaacaaact caggtcgcta
      361 atatctgacc tgaaacaaca tggtcagata ctgctggttg ttgatcagcc agctaccatc
      421 ggtgcgttac ctgtcgccgt tgcccgctca gaaggagtcc ttgtcggata cctccctgga
      481 ctggccatgc gccgcatagc cgacttacac gccggtgaag ctaaaactga tgctcgtgac
      541 gctgccatca ttgccgaagc tgcccgtacc ctgcctcacg cgctacgcac gctgaaactg
      601 gctgacgagc aaatcgccga actctccatg ctctgcggct tcgatgatga tcttgccgca
      661 cagacaacgc aggccagcaa ccgtatccgc ggccttctga cccagataca tccggcactg
      721 gagcgcgttc tcggtccgag acttgagcac ccggcggtac tcgatcttct ccagcgatat
      781 ccctcacc
//
LOCUS       DAZPAC010000549          787 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZPAC010000549 DAZPAC010000000
VERSION     DAZPAC010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.565"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>787
                     /gene="ipaH1.4"
                     /locus_tag="ACHUPM_004861"
     CDS             <1..>787
                     /gene="ipaH1.4"
                     /locus_tag="ACHUPM_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH1.4"
                     /protein_id="HFR0872360.1"
                     /translation="YSLTPFSLPMELTPSCNEFYLKAWSEWEKNGTPGEQRNIAFNRL
                     KICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNLLTHLPDLPPMLKVIHAQFNQL
                     ESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHNNRLHILPLLPPELKLLVVSGN
                     RLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVLMNNNLTTLPESVLRLAQNAFV
                     NVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSATISAPEH"
BASE COUNT          261 a          172 c          133 g          221 t
ORIGIN      
        1 atactcgtta actccatttt ctttacctat ggaactgact ccatcttgta atgaatttta
       61 tttaaaagcc tggagtgaat gggaaaagaa cggtacccca ggcgagcaac gcaatatcgc
      121 cttcaatagg ctgaaaatat gtttacaaaa tcaagaggca gaattaaatt tatctgagtt
      181 agatttaaaa acattaccag atttaccgcc tcagataaca acactggaaa taagaaaaaa
      241 cctattaaca catctccctg atttaccacc aatgcttaag gtaatacatg ctcaatttaa
      301 tcaactggaa agcttacctg ccttacccga gacgttagaa gagcttaatg cgggtgataa
      361 caagataaaa gaattaccat ttcttcctga aaatctaact catttacggg ttcataataa
      421 ccgattgcat attctgccac tattgccacc ggaactaaaa ttactggtag tttctggaaa
      481 cagattagac agcattcccc cctttccaga taagcttgaa gggctggctc tggctaataa
      541 ttttatagaa caactaccgg aattaccttt tagtatgaac agggctgtgc taatgaataa
      601 taatctgaca acacttccgg aaagtgtcct gagattagct cagaatgcct tcgtaaatgt
      661 tgcaggtaat ccattgtctg gccataccat gcgtacacta caacaaataa ccaccggacc
      721 agattattct ggtcctcgaa tatttttctc tatgggaaat tctgccacaa tttccgctcc
      781 agaacac
//
LOCUS       DAZPAC010000550          776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZPAC010000550 DAZPAC010000000
VERSION     DAZPAC010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.566"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..344
                     /locus_tag="ACHUPM_004862"
     CDS             <1..344
                     /locus_tag="ACHUPM_004862"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR0872361.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(406..>776)
                     /locus_tag="ACHUPM_004863"
     CDS             complement(406..>776)
                     /locus_tag="ACHUPM_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HFR0872362.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          210 a          190 c          176 g          200 t
ORIGIN      
        1 gtgcagatgg tcatttttgt ggcaatttgc cgaatacgac ccatttccat gagcaaaccg
       61 tggagttccc gatccaggga gagatggttg atattgtggc gcatggtgag ttgggcggcg
      121 acttttcgtt agtgcctgac agctacgtga cgatggggcc gaaaagcatc atggcggcga
      181 aaaacttgct gataattgtc agtggcgcag gtaaagcgca ggcactgaaa aatgtgctgc
      241 aaggtccggt taccgaagat gttccagctt ccgtgttgca gctgcatcct tcgctgatag
      301 taatcgctga taaagccgct gccgcagaat tagcgctcgg ttaagctgaa gaaataaggc
      361 cggatgtatg aattacgctc cggcctgttc tcattattta aataactacg aaaaatcacc
      421 ccacagtctc agcttgccgc agtggagaca gcggaagaga taaccctgac aatcaccacc
      481 cttttgcagg catttagcta gatcagaatt tctaataccg aaattctcac aatcttcttc
      541 aaggttggca aattcatcga ggcgatcttt tatatcattc cagcccacat agccgataaa
      601 agcacagaaa tcgccacaat gcgcaagcca gaattcctgt tgccatccat gataacctgg
      661 cgtgcgttca accaactctt tcagcatttc atcaggatat gtgttcttaa taccggcaaa
      721 ttcgtcctct tcatcatact caaattcaac accttctatg ctggcatcat cctgaa
//
LOCUS       DAZPAC010000551          770 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZPAC010000551 DAZPAC010000000
VERSION     DAZPAC010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.567"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(56..352)
                     /locus_tag="ACHUPM_004864"
     CDS             complement(56..352)
                     /locus_tag="ACHUPM_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR0872363.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          230 a          164 c          152 g          224 t
ORIGIN      
        1 aggccaaaca agaacacggt tgcaaaaacc gtgcccttaa atattgaatc tctattcaga
       61 acactttctt aaatggtttc actgaaacgt gttcatagac acctgccgct acgtacgggt
      121 cagcatcggc ccaggcctga gctgcttcca gcgattcaaa ttcagcaata acggttgagc
      181 cagtaaatcc cgcagcccct ggatcgttac tgtctaccgc tggcattgga ccagctgtca
      241 acaaacgacc ttcatcatgc agtaactgta aacgtgctaa atgtgccgga cgaacggaaa
      301 ggcgtttttc gaggctatca gctttgtctt gagcgtaaat aacatacaac acgggcacaa
      361 ctccttgttc gggaaagttg taaagtacgt tatttgaaag ggcgaagatc tgcaacggaa
      421 agatgatgtc tttgttaaga ccatgcataa agtaaggtca attacgccaa aaatgacatt
      481 ttcactgatc ctgatcgtct tgccttattg aatatgattg ctatttgcat ttaaaatcga
      541 gacttggttt ttctactgaa atgattatga cttcaatgac ccttgaatga agtctattcg
      601 gttttctagg agacctgtta aaaacaggac aatatcaggt gagtccctat gcaatttaat
      661 agtagatttc caagtatcta tgcaattcta cacagattct atacagtctc ggtatagtcc
      721 ctttttaccc taacttattg aaatatataa agaatctgtg aagtctcttt
//
LOCUS       DAZPAC010000552          767 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZPAC010000552 DAZPAC010000000
VERSION     DAZPAC010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.568"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>767
                     /locus_tag="ACHUPM_004865"
     CDS             <1..>767
                     /locus_tag="ACHUPM_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR0872364.1"
                     /translation="ICCASTRESDHIANMWLSKVVDDEGKEIFSGIRHGVISAYGLKK
                     NSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKIVSTSLLTPTSLTGGEES
                     MLKDQVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVVTFNFGVNELALKMGLGW
                     RNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKNDVIYLANQIKEIINKKL
                     QKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRT"
BASE COUNT          262 a           99 c          196 g          210 t
ORIGIN      
        1 aatttgttgt gcttctacga gagagagtga tcatatcgcg aatatgtggc tttcaaaagt
       61 ggtggatgac gaggggaagg aaattttttc gggtatcaga catggagtta tatcagccta
      121 tggattgaaa aaaaactcat ctgaaagagc tgttgctgct cgtaataaag ctgaggagtt
      181 agtaagcgca gcattatata gcagaccaga gttattatca caggctttgt ctggtaaaac
      241 agtagattta aagattgttt cgacttctct tctgacgccg accagtttaa ccgggggaga
      301 ggagagtatg ttaaaagatc aggtgaacgc attaaaaggg ttaaactcta aaagggggga
      361 gccaacgaaa ttattaattc ggaatagtga tggtcttctg aaagaagtaa gcgttaatct
      421 aaaagtggtg acatttaact ttggtgtaaa tgaattagcg ctaaaaatgg gcttaggctg
      481 gaggaatgtt gacaagctta atgatgaatc aatatgttct ttgttggggg ataatttcct
      541 caaaaatggt gtgattggag gttgggctgc agaagcaata gaaaaaaatc caccatgtaa
      601 gaatgatgtg atatatctgg ctaaccagat aaaagagatt ataaacaaga agttacaaaa
      661 aaatgataat ggagagccgt ataaattgtc acaaagaatg actctattgg cttatactat
      721 aggtgctgta ccttgctgga attgtaagag tgggaaggac agaacag
//
LOCUS       DAZPAC010000553          750 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZPAC010000553 DAZPAC010000000
VERSION     DAZPAC010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.569"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..391)
                     /gene="fadM"
                     /locus_tag="ACHUPM_004866"
     CDS             complement(<1..391)
                     /gene="fadM"
                     /locus_tag="ACHUPM_004866"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR0872365.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQM"
     gene            complement(485..>750)
                     /locus_tag="ACHUPM_004867"
     CDS             complement(485..>750)
                     /locus_tag="ACHUPM_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR0872366.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
BASE COUNT          170 a          202 c          161 g          217 t
ORIGIN      
        1 ccatctgctc cagcttttcg cgcaattccc cttccagagc taatgctttc tgcgttttaa
       61 gatcaataca aacaaacgta ataagcgcat ccgctaccac ctgcccttcc ggctccagtg
      121 taatgacctg gcttaagatg ccgcttttac cgtttaattg ctgcaactga ctggtaatag
      181 ttaacaggtc gcttaatacc gctgggcgac gatagttaat attgatattg accacgacga
      241 aggcgatgtt atgggccgtc atccactgaa aactgtcgct attttccaac ccatcccagc
      301 gggcttcttc gagaaattca aggtagcggg cgttgttgac gtgctggtaa acgtcgagat
      361 gatatccacg aactttgatt tgtgtttgca tagcgcaata accttacgtt gttgttataa
      421 gtacagaggt cataactggt atgacctctt cagtctggca aaaaattgcc actatgcaaa
      481 ttaattacag ggttaatacc gccagattac gttccaccag cgaattgccc atccccggca
      541 cctgctttag atcctccacc gttttaaacg gaccgtactc ttcgcgataa ctgacaatcg
      601 cctgcgcttt cttcaggcca acgccattca tcgcgcgggc tagctcttcc gcgctggcat
      661 tattaatgct gacccgggtg ccttcttcgt cactggcttt cgccggtact gctgctttac
      721 tttgtgctgc aggagcttcc gctttggttt
//
LOCUS       DAZPAC010000554          749 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZPAC010000554 DAZPAC010000000
VERSION     DAZPAC010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.570"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            584..>749
                     /locus_tag="ACHUPM_004868"
     CDS             584..>749
                     /locus_tag="ACHUPM_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR0872367.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVI"
BASE COUNT          212 a          135 c          164 g          238 t
ORIGIN      
        1 cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga
       61 acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa
      121 accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg
      181 agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac
      241 aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa
      301 ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg tcagggatta
      361 aaagatgaac gtaaatttat ttaacgcatt aattttaaag ggttttattg tttgttgacg
      421 aaaacaggaa tcgtgttcgg tctcttttta tctgttaaaa gccagaagca tttccttcgc
      481 tgactttata gtcaaccata acacacactc tactgtctga gtccaacgtt ttttaacatt
      541 cttgttaaaa ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc
      601 cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg gcgatataca
      661 cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc cgtggcgaga
      721 tttacttatc tcagtgggag acgttatcg
//
LOCUS       DAZPAC010000555          746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZPAC010000555 DAZPAC010000000
VERSION     DAZPAC010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.571"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(80..478)
                     /locus_tag="ACHUPM_004869"
                     /pseudo
     CDS             complement(80..478)
                     /locus_tag="ACHUPM_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          158 a          194 c          177 g          217 t
ORIGIN      
        1 gagatgtgga gccagaaaaa tggctgcgtt acgtcattga actgtccttg acccacgata
       61 ttcagacagt cgttgtatct taagttgaca ttacccgctg acgtcgatat tcccttggtg
      121 agagataacc caacgcacta tgcggatggt tttcattgta atgctcgatc gccactgcaa
      181 ggttatgcaa tgccgttctt ggtttcggca tgaacgcgat gcagtcttcc ttcatcgttt
      241 tcacgaaccg ttctgctatt ccattactct gagggctact gactgccgtc gtgcagggct
      301 ccagattcaa ctctctggcg aactgccgtg tttcatgagc cgtataagcc gaaccgttat
      361 cggccactgg attggccgtt ccggtccttt atcgccatat cgctgacatg acatcctgca
      421 ccgttgcttt gtcataatct cccgtgcctg ctgcccagtc tatggcttca cgatcgcaat
      481 atggaacgta acgctgtcat cgggagaatc gtcaatgtgg cattcggtta acgctgatat
      541 gactttccgt gtcaaaccgg aatctctaat tttgacgatt ttctttttag gtttaattcc
      601 ctgagggcga cgtagcagta aatccgacgg tgaatcacgc tgatattcgc gggttggata
      661 ccgcattaca gaggtccgtc atatgagcct gccactttct tacgtcgcga atcagctttg
      721 ttccgccgtc gccaagcgct aacgtg
//
LOCUS       DAZPAC010000556          745 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZPAC010000556 DAZPAC010000000
VERSION     DAZPAC010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.572"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>745
                     /locus_tag="ACHUPM_004870"
     CDS             <1..>745
                     /locus_tag="ACHUPM_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFR0872368.1"
                     /translation="NLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPEAP
                     VAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFKQGP
                     TGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINE
                     MDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAELKNI
                     EEREGKTFHYYSLAVMISAKQINNLISQE"
BASE COUNT          236 a          160 c          175 g          174 t
ORIGIN      
        1 ggaatttatt atcttccgct attatagtcg ccatcatggc cctcggtctg acgggttgtg
       61 atgataaaaa agccgaaaca gaaacgcccc cgcctgccaa tagtcaacct gccgcaccag
      121 ctcctgaagc tcccgttgca aaagcagaag ctaaacctga aacacctgcg caaccggtgg
      181 tcgatgaaca agcggttttc gacgaaaaaa tggatgtcta tatcaagtgc tacaacaagt
      241 tacagatccc tgtacagaac agtctggcgc gttatgctga ctggctgaaa gattttaaac
      301 aggggcctac cggtaaagag agtactgttt atggcatcta cggcattagt gaatccaacc
      361 tcgctgagtg tgaaaaaggc gtaaaaagtg ctgtggcgtt aacgcctgcg ctgcaaccta
      421 ttgatggcgt ggcggtgagt tatattgatg ctgccgtggc gttgggtaat accattaacg
      481 aaatggataa atattacacc caggaaaatt ataaagacga tgcgtttgcg aaagggaaaa
      541 cgctgcacca gacattctta aaaaatctgg aatcctttga acctgtagca gaatcttatc
      601 atgcggcgat tcaggaaatt aatgataagc gccagcttgc ggaactgaaa aatattgaag
      661 aaagagaagg gaaaacattc cactactact ctctggcagt catgatttca gcgaaacaaa
      721 ttaataacct gatttcgcaa gagaa
//
LOCUS       DAZPAC010000557          743 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZPAC010000557 DAZPAC010000000
VERSION     DAZPAC010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.573"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..396)
                     /locus_tag="ACHUPM_004871"
     CDS             complement(<1..396)
                     /locus_tag="ACHUPM_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0872369.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          233 a          172 c          108 g          230 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcattcatgt caccaaatcg
      541 tttatttagt gagataaaac tagcatattc gtaagataaa aaatgcacat ggcaatcaag
      601 aaatgatatt attacttttt tgatgtaaaa ctatttagtt ttaaaattca ttttttcaac
      661 atatagagca ttcattttat gaatgcaaaa aaacatacct gacacacaaa ttactctcct
      721 ctgtaatatt ttctcattaa cac
//
LOCUS       DAZPAC010000558          743 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZPAC010000558 DAZPAC010000000
VERSION     DAZPAC010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.574"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..721
                     /locus_tag="ACHUPM_004872"
     CDS             <1..721
                     /locus_tag="ACHUPM_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0872370.1"
                     /translation="NNLSWFIYKYDELAELAAVIDRLYEFHQLTEQRPTNKPKNCQHA
                     VQVANASIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDI
                     SSPADSWYVSQTPLIKSGLLKEIICKALPLPVDDKSLSEVLHQVGLGKLAARIHDHDR
                     WGDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLRLVREKLPTSGVIM
                     VTHQPGVWNLADDICDISTVL"
BASE COUNT          214 a          162 c          164 g          203 t
ORIGIN      
        1 gaacaattta agctggttta tttataaata tgacgaactt gctgaactgg ctgcggttat
       61 cgatcgcttg tatgagttcc atcaactcac tgaacagcgc cctacgaata agcctaaaaa
      121 ttgccaacat gcggtacaag tggctaatgc gagtattcgt acgcctgata ataagatcat
      181 attagagaac ttgaactttc atgtttcgcc aggcaaatgg ctattactga aaggctactc
      241 tggtgcggga aaaaccacac tgcttaaaac attatcccac tgctggccgt ggtttaaagg
      301 tgatatttct tctcctgctg acagttggta tgtgtcacaa acaccgttaa tcaaaagcgg
      361 cttactgaaa gagattattt gtaaagcact tcccctgccc gtagacgata aatcgttgag
      421 cgaagtactg catcaggttg gtctggggaa attggctgcg cgtattcatg accacgatcg
      481 ctggggagat attctttcca gcggcgaaaa acaacgtatc gccctggcac gattaatttt
      541 acgacgtccg aaatggatat ttcttgacga aactacctct catcttgagg aacaagaggc
      601 tatacgctta ctgcgtttag tgcgtgaaaa actacccaca agcggcgtca ttatggttac
      661 acatcaaccc ggtgtctgga acctggctga tgatatttgt gacattagca cggttttata
      721 acaacaataa aagccctgct gta
//
LOCUS       DAZPAC010000559          740 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZPAC010000559 DAZPAC010000000
VERSION     DAZPAC010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.575"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..516)
                     /locus_tag="ACHUPM_004873"
     CDS             complement(<1..516)
                     /locus_tag="ACHUPM_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR0872371.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVNKNISH
                     HGKNYGFQLTPA"
BASE COUNT          202 a          149 c          104 g          285 t
ORIGIN      
        1 ggcaggtgtt aattgaaaac cataattttt cccatgatga ctaatattct tgttgaccgg
       61 tccccaattc ttattattta aataatcagt aaatatttgt tttattttcg tttgcaattc
      121 ctttttattt ttaatgccta gctgtgctct tatctcttca cttattactt tattaagctc
      181 attaatatgt cttttattaa cttcaccaag tttataggta tctaatcgtt ccatcatggc
      241 tttaattttt gacgtatcac cacctgccaa ctttattgcc tcgtaaaaaa cttcagctct
      301 gtcacgcgca aacaaaaggc tttttcctgt taagcttgtt tggttctgta aatataatcg
      361 attaagcgct tcgatcccct tatttctttc tccattttta actctttgat atgaaatcac
      421 gcttacacct ttgtgctttc cggtttcttc tgcgccttta taggaatctc cgctttgaaa
      481 tgaaacctga tgcaatccca aattagttat gtgcatttaa ttatcctcag tcataaatgg
      541 ggcattgatt gcagttttgt acctcgtttt acaacaaaaa agatacttat tcaacagggt
      601 tctctctttt tctataacac tccgagcctg atatatcaga cactttaact tgtgggattt
      661 catgatgaaa cgagcactac atatgctcac gaggtacata ttatgtacaa caaaagaaaa
      721 tttgattatg ttaacaggct
//
LOCUS       DAZPAC010000560          731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZPAC010000560 DAZPAC010000000
VERSION     DAZPAC010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.576"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>731)
                     /locus_tag="ACHUPM_004874"
     CDS             complement(<1..>731)
                     /locus_tag="ACHUPM_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR0872372.1"
                     /translation="KAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFG
                     HNGVISWGSTAGFGDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAETF
                     TVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQA
                     LTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNP
                     LSGYIANWNNSPQKDYPASDLFAFL"
BASE COUNT          194 a          195 c          155 g          187 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccaacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg caaatggtgt attgccagtg acatcataac cagcaccgtg
      661 cagaccaata ccataagtat acgcaggcgc ataccagcca aactgcggac catttaccat
      721 gattgctttc g
//
LOCUS       DAZPAC010000561          731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZPAC010000561 DAZPAC010000000
VERSION     DAZPAC010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.577"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..239
                     /locus_tag="ACHUPM_004875"
     CDS             <1..239
                     /locus_tag="ACHUPM_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR0872373.1"
                     /translation="ADRNRSHWSTSKTPSYTKSVSWQHHPLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            344..535
                     /locus_tag="ACHUPM_004876"
     CDS             344..535
                     /locus_tag="ACHUPM_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR0872374.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(536..>731)
                     /locus_tag="ACHUPM_004877"
     CDS             complement(536..>731)
                     /locus_tag="ACHUPM_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR0872375.1"
                     /translation="DVDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGN
                     LHYIDTGAVFGGELTLVQLQ"
BASE COUNT          189 a          185 c          161 g          196 t
ORIGIN      
        1 cagctgatag aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag
       61 taagttggca gcatcacccg ttaaccattc cttgtaaatc tgaccccgat ttatgtatgc
      121 aactcgatgc ctgggatgct gaaaccagta tccctgccct gctaaatggc gaacactctg
      181 tcctttaccg tacccgttac gatcaacaat ctgatgcctg gattatgcgt cttgcctgat
      241 ccaaaaagaa cccgtcggga tggcgggtta tttgtcctgg ttattccccc gttgtaaaat
      301 ctctcctaaa cttaacggta cggcaccaca cttcggggat gaaatgttcg cgctggtact
      361 ttttgtttgc tacctggatg gcggttgtga agatattgtt gtggatgtct acaacacgga
      421 acagcagtgt ctttattcta tgagcgatca acggatccgc catggcggtt gttttccgat
      481 tgaggatttt atagatggtt tctggcgacc tgcacaggag tacggtgatt tttaattatt
      541 gcaattgcac aagagtcagt tcgcccccaa agacagcacc ggtatcaata taatgcaggt
      601 tgccaatatc cacgcgatgt cgcaacggtg tatgaccaaa ccagaaatga tcagcacctg
      661 taattccctg ccctttttgg cgttcaccta atcgcgagcg gctccacaag acctgatgca
      721 aatcaacgtc c
//
LOCUS       DAZPAC010000562          726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZPAC010000562 DAZPAC010000000
VERSION     DAZPAC010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.578"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            35..>726
                     /locus_tag="ACHUPM_004878"
     CDS             join(35..284,284..>726)
                     /locus_tag="ACHUPM_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFR0872376.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHY"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAZPAC010000563          725 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZPAC010000563 DAZPAC010000000
VERSION     DAZPAC010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.579"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..429
                     /locus_tag="ACHUPM_004879"
                     /pseudo
     CDS             1..429
                     /locus_tag="ACHUPM_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          169 a          204 c          213 g          139 t
ORIGIN      
        1 cctgtggaaa aaatacggca gtggcgataa cggtactccc ggccgatagt gaatgacctg
       61 tttgcctggc ttgaagagca ggagccgtgc tgtccgcccg acgggccatt aaataaggcg
      121 attaactata ttctgaaccg gcgcgatgaa ctgagctgct tcctgggtga cggagcggtg
      181 cccctggaca acaatatctg cgagcgtgcc attcgcccgg ttgttatggg acggaaagcg
      241 tggctgttcg ccggttcact gatggccgga aaccgggcgg cacagataat gagccttctg
      301 taaacagcaa aacgtaatgg tctggagcca catgcctggc tgacggacgt cctgacgcgt
      361 ctgccggaat ggccggagga gcgactggca gagttgctgc ctcttgaggg atttaccttc
      421 accgggtgag tgatacctgc cgtcaggtgt tcgtgaaccg ggctataacc agcagtcggt
      481 tgttgaacgc ctgacaacag gaaatcagcc aggccagcaa ccacgcggac tgacagagaa
      541 tcagaacctg aatcagcctg aaatcatacc cggggcagta cttcatcacc agtcaccaat
      601 acccgtgtca gtacttcgcg gcctgacgcg atcacccgac tcagtacttc tgagcccgcc
      661 tccggtacca ttctctgtgg gcccgtacac aacaacagaa aagggggccc ggcccggggc
      721 gcaac
//
LOCUS       DAZPAC010000564          716 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZPAC010000564 DAZPAC010000000
VERSION     DAZPAC010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.580"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..628
                     /gene="rnhA"
                     /locus_tag="ACHUPM_004880"
     CDS             <1..628
                     /gene="rnhA"
                     /locus_tag="ACHUPM_004880"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR0872377.1"
                     /translation="KIGAALDSGVYMTTKTPARPQAKTRKKDKNKNSPRTNPTTPVVE
                     FNPQLKTVKIFSDGSCLKNPGGPGGYGIVLQYRGEEREFSDGFHSTTNNRMEMMGALI
                     GLERLKYPCNVILHSDSQYLKNGMTQWMKWWKRNGWMTSDKKPVKNVDLWKRLDEAAS
                     RHNVRWKWVKGHAGHRENEICDRLAKIAAFSAADMPHKKDIGFVYNKQ"
BASE COUNT          220 a          151 c          177 g          168 t
ORIGIN      
        1 aaaaatcggc gcggcgcttg attcaggagt ttatatgaca acgaagacac cagcccgacc
       61 gcaagcaaaa actcgcaaaa aagacaaaaa caaaaattct ccccgcacca atcccacaac
      121 gcctgtcgta gagttcaatc cccagcttaa aaccgtgaaa atctttagtg atggctcttg
      181 ccttaaaaat ccgggtggcc cgggcggtta cggtatcgtt ctccagtatc gtggcgagga
      241 acgcgagttc tcagatggtt ttcatagcac caccaataac cgcatggaga tgatgggggc
      301 acttatcggg ctggagcgtt tgaaatatcc atgcaacgtt attttgcact ctgatagcca
      361 gtatctgaaa aacggcatga cacagtggat gaaatggtgg aaacgcaatg gatggatgac
      421 ttctgacaaa aaaccggtaa agaatgttga tctgtggaag cgtctggatg aggccgcaag
      481 tcgacataat gttcgctgga agtgggttaa aggtcacgcc gggcatcgtg aaaatgaaat
      541 atgtgatcga ctcgcgaaga tcgcagcttt ttcagcagca gatatgcctc acaagaaaga
      601 tattggtttt gtttataaca aacagtaagt aagtgtttac ctatcattat aaatcatgta
      661 tcttatcggc gtcaggatga caatgtgtcg gtaagacaca gttccaggat ggaacg
//
LOCUS       DAZPAC010000565          710 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZPAC010000565 DAZPAC010000000
VERSION     DAZPAC010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.581"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
BASE COUNT          245 a          109 c          147 g          209 t
ORIGIN      
        1 gatcgattca gatgccattt tgcagtagtc tattttataa aaaaattaag taggctaaga
       61 agtaccttag aaatcaattt taaggggtct aaaacgctct gtaacgagct aaactgtata
      121 agtggcagta agatgccttt tagactgaca ctcgcttaga gagcttccta gagcttctgg
      181 gaggattcat tagatatcgt tgtatacaga gacacagtat ctataaaggg tattaagaga
      241 ttcttagaag ttactgtaaa gaaccactaa gataagaaga gagggacaaa gaaccgaagg
      301 aaagtccctt attaagtagg agtcttaata gactgtctta atagataact taaaagtctt
      361 aaaggatatc ttccaagaag agaggaacga agaaccagag gaagtgaccg ttgaccgttt
      421 gaacagactg tttaagtaaa gcctataaag tatctattaa ttaagggcta tgtctcaata
      481 ctagcagttt gtcaatacct ttgtctttag caattcgtgc tatttattaa atatagcact
      541 ttttgttaaa acagagtatg ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca
      601 tgttcttggg ttcttagtga gagttacact atcttctaag gtactcaata aaaagacgag
      661 taacaaccga agaacgagga tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAZPAC010000566          701 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZPAC010000566 DAZPAC010000000
VERSION     DAZPAC010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.582"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            9..494
                     /gene="sixA"
                     /locus_tag="ACHUPM_004881"
     CDS             9..494
                     /gene="sixA"
                     /locus_tag="ACHUPM_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR0872378.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT          169 a          165 c          175 g          192 t
ORIGIN      
        1 ggtgcaatat gcaagttttt atcatgcgtc acggcgacgc agcccttgat gccgccagtg
       61 attcggttcg tcctctgacc actaatggtt gtgacgaatc tcgcctgatg gcgaactggc
      121 tgaaaggtca aaaagtggaa atcgaacgcg ttctggtgag cccgttcctg cgagccgagc
      181 aaacactgga agaagtaggg gattgtctga acctgccttc cagtgcagaa gttctgccgg
      241 agttaacgcc ctgcggcgat gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg
      301 aaggtgttgc ttcggtactg gtgatctctc acttaccgtt agtcgggtat ctggttgccg
      361 agttatgtcc gggcgaaacg ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc
      421 ttgatgaaag cggaaacggc acattcaact ggcagatgag cccgtgtaat ctgaagatgg
      481 caaaagctat ctgatgagtt ccaaattatg cccaatgagg tagattcatt gggcataatt
      541 gctcatcagg cagcggatat ctgtcaatct cactactatc gaagactctc cccatctata
      601 aataaaacac tttctacttt cattcggaaa ttattcttct gcgcgtatct tgattttgcc
      661 caaagttaca tgttgaaatg aataatgtct gtttgacact c
//
LOCUS       DAZPAC010000567          697 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZPAC010000567 DAZPAC010000000
VERSION     DAZPAC010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.583"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..320
                     /gene="ospI"
                     /locus_tag="ACHUPM_004882"
     CDS             <1..320
                     /gene="ospI"
                     /locus_tag="ACHUPM_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFR0872379.1"
                     /translation="VSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFN
                     DGEKIMFLDVQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSK
                     RLC"
     gene            435..697
                     /locus_tag="ACHUPM_004883"
                     /pseudo
     CDS             435..697
                     /locus_tag="ACHUPM_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          214 a          117 c          165 g          201 t
ORIGIN      
        1 tagtgtcaca tcctaatgaa acttatagta aatttcgtga gcgtattgca gaaaatattt
       61 tacaaaacac ttcaaaagga agtgtagtaa tgatttctat tgaacaggcc actcattgga
      121 tagctggatt taacgatggt gaaaaaatta tgtttttgga tgttcaaaca ggaaagggat
      181 ttaatcttta tgatcctgta gaaaaatcgc cagatgcatt tgtagatgaa aatagttcgg
      241 ttcaggtaat tcatgtatct gatcaggaat ttgatcacta tgctaacagt agtagttgga
      301 agagtaagag gctttgctga tgctgagagc ctgtacatag atttgagccg gccccttgaa
      361 acgccagaca gtggatgtat cttaaaataa gagataggct tggatatatg tctaacacta
      421 gttctaattt tgagatgact ggaactttgc tggggctgga gcttcgtaaa cgtaaaactc
      481 ctcaggaaaa aattgccatt attcagcaga caatggagcc tggcatgacg gtgtcccatg
      541 tggcgcgttt gcatggtatt cagcccagcc tgttgcttaa gtggaaaaaa taatatctgg
      601 aaggaagtct cactgctgtt gctgccggag aggatgtcgt tcctgcctca gaactggctg
      661 ccgctataaa acaaataaaa gagttacagc gcttgct
//
LOCUS       DAZPAC010000568          695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZPAC010000568 DAZPAC010000000
VERSION     DAZPAC010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.584"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>695
                     /locus_tag="ACHUPM_004884"
     CDS             <1..>695
                     /locus_tag="ACHUPM_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0872380.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAZPAC010000569          686 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZPAC010000569 DAZPAC010000000
VERSION     DAZPAC010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.585"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..686
                     /gene="espX1"
                     /locus_tag="ACHUPM_004885"
                     /pseudo
     CDS             1..686
                     /gene="espX1"
                     /locus_tag="ACHUPM_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
BASE COUNT          269 a          117 c           98 g          202 t
ORIGIN      
        1 aacatacagt tcaattttat tttttaaaca caaaggtatc acattactta gattggatgt
       61 ttttgatgac agaacaagcg aatgtctttc tgaagagatt aagatatacc aagaatgcca
      121 tgaaaaattc gttaattttt taaaggcaaa cttcaaccaa gaaatatacc cacagctata
      181 tactcctgaa atattttatg aggcctgcag aaatctccag tcattttatg atcatcagga
      241 aacgtcacaa aacgcaaaat acagtgcaat agacaagaaa aaaagtcatc ttaaccagga
      301 aatcaaaaag cttattaaaa aaaatatttt ccctgagcta tacaatgagc aatgcaataa
      361 aatcccctcc tcatcgatag atgataacca gggaataatg tggcagaact ttaaaactag
      421 caattcggca tattcaaaac tatgtggaaa attatcttta tttaaaagcg tgcccagtcg
      481 gttaatagaa aaatcagctt actgctcaac tgaaaacatg gccactaata aatttaatgt
      541 cgtcttttct tactgcggag ataatataaa ggaatttata cttttattac cctataataa
      601 atcactggag atgtatgaac ttaacgatca aaagatccag tatttgactg ctcttaatat
      661 taatatacat aaaatgttgc tatcaa
//
LOCUS       DAZPAC010000570          684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZPAC010000570 DAZPAC010000000
VERSION     DAZPAC010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.586"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..560
                     /locus_tag="ACHUPM_004886"
     CDS             <1..560
                     /locus_tag="ACHUPM_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR0872381.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
     gene            complement(616..>684)
                     /locus_tag="ACHUPM_004887"
     CDS             complement(616..>684)
                     /locus_tag="ACHUPM_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872382.1"
                     /translation="ALMMITYIPQISLFIPQLLGLM"
BASE COUNT          244 a          122 c          165 g          153 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acgc
//
LOCUS       DAZPAC010000571          682 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZPAC010000571 DAZPAC010000000
VERSION     DAZPAC010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.587"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            124..>682
                     /locus_tag="ACHUPM_004888"
     CDS             124..>682
                     /locus_tag="ACHUPM_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR0872383.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRL"
BASE COUNT          189 a          154 c          192 g          147 t
ORIGIN      
        1 taaacacgga gcagtatgat ggttaaaagg gtgaaactgg caggtgtgga gcctcgcaga
       61 tttccggcat catagtgccc aatacgggat gcataaagct ccctcagact ggagactcca
      121 caaatgaaat atacaccggt tggcgttgat atcgcaaaac atgtcattca gattcacttc
      181 atcaatgagc acacaggtga agtggttgat aaacagttgc gtagacagga ttttctgacg
      241 ttcttcggca accgtgagcc atgcctgatt ggtatggagg cctgtggagg ttctcagcac
      301 tgggcacggg aactgacaaa acttggtcat aaagtccggt tgttgcaggc ccgcttcgtt
      361 aaggcattcg tcatgggcaa taagaatgat gtgatggatg cccgggctat ctggatggcg
      421 gttcagcagc cgggtaaaga aatcgccgta aaaacagaag aacagcagtc ggtactggtt
      481 ctgcaccgta cccgcatgca actggtgaag ttccggaccg cacaaattaa tgccctgcac
      541 gggacgttac tggagtttgg tgaaaccatc cacaaaggcc gggcagcgat ggagcgggag
      601 ttccccgaag cactggaacg gatgaaagag agactgccac cgtatctcat tatggttctg
      661 gaaaaccagt acaaccgact ga
//
LOCUS       DAZPAC010000572          677 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZPAC010000572 DAZPAC010000000
VERSION     DAZPAC010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.588"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>677
                     /locus_tag="ACHUPM_004889"
     CDS             <1..>677
                     /locus_tag="ACHUPM_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0872384.1"
                     /translation="QMQSQMDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWCAEYD
                     EWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPI
                     DQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQ
                     TNWSTTQ"
BASE COUNT          194 a          170 c          171 g          142 t
ORIGIN      
        1 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaattc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggccct tatcagcacg gaagggacaa cagcgtggtg
      121 cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc atctgcagca
      181 gctcattcgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccggttcag tatatagatt caatggggct
      361 ggcatctaaa tatggacact taaataatgg cggatatgga gcgaaaccca gcaaaccgcc
      421 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta tgccgcagga
      601 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggtcaaca actcaat
//
LOCUS       DAZPAC010000573          666 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZPAC010000573 DAZPAC010000000
VERSION     DAZPAC010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.589"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
BASE COUNT          210 a          159 c          131 g          166 t
ORIGIN      
        1 gacagacctg tgctatcgga aagaaaaaag gccatcagca aaactctgat ggcctgaatc
       61 acctttacca atattgtatg aaaaaacacg cacgactaat tgacaataat tttcatttcc
      121 attaaaaaag atcgcgtagc attcttaatt catgaggaac tttacccgcc agcaatctga
      181 gtagcgaaag ctgttcatcc ccaatgtttt tgctggcggg tccttttttc tacggtcctc
      241 ctccccggag gggggcattt cattattttt ctaatcgtta ctggtgagaa ggcggaagaa
      301 aaagccagtt cttcgcccct aaataacagc atgattccag tccatgcaaa atctgatttc
      361 cagctcacac ataccgagcg ctgtacaaaa aaccgccagc caaggcagtt aagagtgtgt
      421 tcccggggtt tgcttagaat attttgtaag ttgtccgaag agatatttac aacaccagaa
      481 tgatgaatca tcagtcccct gccagaggtt cactacgtaa cctgaccgac aatgcaccat
      541 tctggtgctg aaaaaagagc actggaactg caacaaaaca acatcacaga acaggagatg
      601 gtaaggagtc agacattccc acacaatatt gtgtcagtgc aaataacaac cttcgtctca
      661 aatctc
//
LOCUS       DAZPAC010000574          662 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZPAC010000574 DAZPAC010000000
VERSION     DAZPAC010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.590"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>662
                     /locus_tag="ACHUPM_004890"
     CDS             <1..>662
                     /locus_tag="ACHUPM_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR0872385.1"
                     /translation="APDGDIIRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFT
                     DCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGD
                     LTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYD
                     VLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVNGETAER
                     WQ"
BASE COUNT          164 a          199 c          191 g          108 t
ORIGIN      
        1 gcccctgacg gcgatatcat ccgctaccgt tatgataatc cacacagtga cttaccctgc
       61 gcaacggaag atgccaccgg cagccggaaa accatgacgt ggagccgtta cggtcagttg
      121 ctgagcttca ccgactgttc cggttatgta acccgttatg accatgaccg cttcgggcag
      181 atgacggcgg tgcaccgcga ggaagggctg agtcagtacc gcgcatacga cagccgtgga
      241 cagttaattg ccgtgaaaga cacgcagggc catgaaacgc ggtatgaata caacgccgcc
      301 ggtgacctga ccaccgtcat tgccccggac ggcagcagaa acgggacaca gtacgatgcg
      361 tggggaaaag ctatctgtac cacgcagggc ggtctgacgc gcagtatgga atacgatgct
      421 gccggacggg tcatccgcct gaccagtgaa aacggcagcc acaccacctt ccgttacgat
      481 gtactcgacc tgctgataca ggaaaccggc tttgacggcc gcacacagcg ttatcaccac
      541 gacctgaccg gcaaacttat ccgcagcgag gatgaggggc tggtcaccca ctggcactat
      601 gacgaagcag accgcctcac gcaccgcacc gtgaatggcg aaaccgcaga gcggtggcag
      661 ta
//
LOCUS       DAZPAC010000575          657 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZPAC010000575 DAZPAC010000000
VERSION     DAZPAC010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.591"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>657)
                     /locus_tag="ACHUPM_004891"
     CDS             complement(<1..>657)
                     /locus_tag="ACHUPM_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR0872386.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     RVPGADEVLPPEPPAYRVLTGVVDGFGRTLAFHRAAEGDVAGAVTGVMDGAGRRFHLV
                     LTTQAQRAEEARKPHTASLSSPDSPCPLSAPSFPDTLPAGTEYGADNGIRLEAVWLTH
                     DPAYPDEQPTAPLARYTYTAGGELRAVYDRSGMQVRGFTYDAEHAGRMVAHHYAGRPE
                     S"
BASE COUNT          105 a          248 c          202 g          102 t
ORIGIN      
        1 gctctccggc ctccccgcat aatggtgcgc caccatccgc ccggcgtgct ccgcatcata
       61 agtaaacccg cgcacctgca tcccgctgcg gtcatacacc gcccgcagtt caccaccggc
      121 cgtgtacgtg tagcgcgcca gcggcgcagt cggctgttca tccgggtatg ccgggtcgtg
      181 tgtcagccat accgcctcca gccggatacc gttatcggca ccgtactctg taccggcggg
      241 cagcgtgtcg gggaaggaag gagctgagag agggcaggga ctgtcagggg aagataacga
      301 agcggtgtgc ggtttacggg cctcttccgc ccgctgcgcc tgtgtggtca gcaccaggtg
      361 aaaacggcgg cccgcgccgt ccatcacccc cgtcaccgcg cccgccacgt caccctccgc
      421 tgcccggtga aaggccagcg tccgcccgaa gccgtccacc acgcccgtca gcacccggta
      481 tgcgggcggc tccggcggca gcacctcgtc cgcccccggc acccgctccg gccagctgag
      541 tatccaccac ggcccctgaa ggctgtttgt cgccaggtac acatgcggac tcaggcgaac
      601 atcttccggc agcacctgcc agagcgcgct tagcggctgc gaactgtgct gcgccgc
//
LOCUS       DAZPAC010000576          655 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZPAC010000576 DAZPAC010000000
VERSION     DAZPAC010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.592"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            47..>655
                     /gene="virB"
                     /locus_tag="ACHUPM_004892"
     CDS             47..>655
                     /gene="virB"
                     /locus_tag="ACHUPM_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFR0872387.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGL"
BASE COUNT          244 a          117 c          117 g          177 t
ORIGIN      
        1 tttaactttt gtcaatataa atcaatatga ataaacaggg tgtgatatgg tggatttgtg
       61 caacgacttg ttaagtataa aggaaggcca aaagaaagag tttacactcc attctggtaa
      121 taaagtttcc tttatcaaag ccaagattcc tcataaaagg atccaagatt taaccttcgt
      181 caaccaaaaa acgaatgtac gcgatcaaga atccctaaca gaagaatcat tagccgatat
      241 cataaaaact ataaagctac aacaattctt ccctgtaata ggaagggaga ttgatggtag
      301 aattgaaatt ctggatggca ctcgtagaag agcatctgca atatatgcag gagcagatct
      361 tgaagttcta tattcaaaag aatatatatc tactcttgat gccagaaaac tagcaaacga
      421 tatacaaaca gcaaaagagc atagcatccg agaacttggt atcggtctta attttctgaa
      481 agtatcaggg atgtcctata aagacatagc caaaaaagag aatctgtctc gcgcgaaagt
      541 cactcgtgcc tttcaggcag caagcgttcc acaggaaata atatctctat ttccaatcgc
      601 gtcagaactt aactttaatg actacaagat attattcaat tattataaag gactt
//
LOCUS       DAZPAC010000577          650 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZPAC010000577 DAZPAC010000000
VERSION     DAZPAC010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.593"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            187..354
                     /locus_tag="ACHUPM_004893"
     CDS             187..354
                     /locus_tag="ACHUPM_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR0872388.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT          194 a          143 c          103 g          210 t
ORIGIN      
        1 aaaacataac cccttaataa atattatctg ctaatgggtt atattcaccg ttacagcgac
       61 ttacaaaatt aagtcattcg ctcactgacg ccaattaaat attcctgtat cgttcttaac
      121 gagcagtctg cttatattca tataatcaat gaatattaat taatatataa tacataggga
      181 atgtaaatga acaaatttat taaagttgca ctggtaggtg cagtactggc tacgttaact
      241 gcatgtactg gtcatattga aaaccgtgat aagaactgct cttacgacta cctgctgcac
      301 ccggcaattt ctatttctaa aatcattggc ggttgcggtc ctactgcaca gtaagtctct
      361 gacagaaatc ggctaacacc ggtttaattg cccgacgccc tctggatttt ccgagggcgt
      421 atttgtttct atgtccttct tgcccccgcc cgtaaaaata ttttcacttt aaattcaatt
      481 tgataactac atccattagc cacagcgcga tcctgtgcga aattttgtga tcttcctcca
      541 cattacataa catcatatgt tgttatattc atcatgcatt gtcatgttac cttttaaatg
      601 actgcaaact ctcccctaca acgtattgga caagaaaaag gtatcgctat
//
LOCUS       DAZPAC010000578          650 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZPAC010000578 DAZPAC010000000
VERSION     DAZPAC010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.594"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..545
                     /gene="ospB"
                     /locus_tag="ACHUPM_004894"
     CDS             <1..545
                     /gene="ospB"
                     /locus_tag="ACHUPM_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFR0872389.1"
                     /translation="DDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQL
                     GLGSELIDVQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSL
                     PFFKEKESLLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADP
                     EHTVKRSSQKKTFIINKELD"
BASE COUNT          219 a          106 c          113 g          212 t
ORIGIN      
        1 atgatgacgc attgaatgct gctgcttatt tttctgtaaa aaaagcgaaa ataaaaacag
       61 taaacgatac tgattttaaa gagtataata aggtttatat tcttgggcac ggtagtcctg
      121 gttctcatca attaggcctt ggttcggaac ttattgatgt acaaacaatc atttcaagaa
      181 tgaaagactg tggtattcta aatgtgaaag atatccgttt tacttcatgc ggctccgctg
      241 ataaagtggc tcctaaaaat tttaacaatg cccctgctga aagtctttct tgtatcctta
      301 actctctgcc tttttttaag gaaaaagaat ctttgctaga gcagataaaa aaacaccttg
      361 aaaacgatga gtcattgagt gatggtctaa aaatatccgg ctatcatgga tatggagttc
      421 actatggtca agagcttttt ccctactcac attatcgttc aacttcaatt cctgctgatc
      481 cggagcatac agtaaaaaga agctctcaga aaaagacttt tattattaat aaagaactgg
      541 attagtataa aatttttaac ctatagttaa ttgtttgcat ttaagatgaa gttattttat
      601 ctttagtaga aaagtgcttc cctatgaggg aaaatcacaa atcatcataa
//
LOCUS       DAZPAC010000579          648 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZPAC010000579 DAZPAC010000000
VERSION     DAZPAC010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.595"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..612)
                     /gene="mxiE"
                     /locus_tag="ACHUPM_004895"
     CDS             complement(<1..612)
                     /gene="mxiE"
                     /locus_tag="ACHUPM_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFR0872390.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVK"
BASE COUNT          212 a          129 c           93 g          214 t
ORIGIN      
        1 tttcactaaa aaagtaatat tcgataattc tcttgcagaa aagcctagtc gtgtttttat
       61 ctcatttgaa aaatgggatg ttgatgcata cccattattc atggcagcag aagtgattgt
      121 ttgattgtgc aaaaatacat ctaataaacc atttactaac cgccaagtgt tcagttgttc
      181 tttgactttt gcccctaaag cttttctaca taaagatcta aaataagcct ccgacacacc
      241 ataatgctct gtcagactct ttattcttac ctccttttct ttctcaatag ttctcagaag
      301 ataaagaacc aaatgatatg actcagtcat tcgtatcata gccagtgcat tacttacatt
      361 ctggtcagat gaaaagatga atcttaacat taacttatca agcatcgatt cggtattaca
      421 actatcccga agatcattct catgatagaa atgaatacat ttttcagcat ggctatgctc
      481 tactagagca ccactgaaat agtcccaaaa agcaagaaat tttgcacaac tattaatgtt
      541 aaagcgataa tgatagttca cattatcact gaattttata ttttgatttc ggacaaaaaa
      601 aaacccttcc atgctggagc cagctcgatt atgttacgtt ctttatct
//
LOCUS       DAZPAC010000580          645 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZPAC010000580 DAZPAC010000000
VERSION     DAZPAC010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.596"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
BASE COUNT          207 a          104 c          136 g          198 t
ORIGIN      
        1 aaaatactat tttaggtgat gcattaaatt agtagaatat ttattctttt ataaaagcac
       61 caatgaattg tccaagtaaa catgaggtaa aatgtgtaga ggataactga gtttggtcac
      121 tttaactgta ttagtcgcag tctgactctc gactttaaaa ggatgggaac tacctgtact
      181 gatagtgaga aagctttcca ttgaaataag tcaaataaaa gttgatggct tcagtgctaa
      241 agtgaggtgc ccgctagccg tattcactcg aatcggaatt caatgtattg cttctgccat
      301 ttccataata tttcacggga aaaacaaaat ctgaaaaact atacttaagt gttgtattga
      361 gtcagttgag atacaaatac atatttatca gcacacgtta cgtcaaatac aggaggttgt
      421 catgttggta ctgaaaggca cgttggctat tgcagccgtg atgacaattt attgtcttat
      481 cgttgttctt atggaacgct tttctgattg atttcatatt ggcgagagaa caagaaggtg
      541 agtagaattg tcgcggaatc ctgaaactat cagcctcagg tatgaacacc aaaaggtagg
      601 tagagaaatc tatggccccc gttactttat tcgcacaatg aggta
//
LOCUS       DAZPAC010000581          645 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZPAC010000581 DAZPAC010000000
VERSION     DAZPAC010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.597"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>645
                     /gene="istA"
                     /locus_tag="ACHUPM_004896"
     CDS             <1..>645
                     /gene="istA"
                     /locus_tag="ACHUPM_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HFR0872391.1"
                     /translation="QLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSLSWPLPADI
                     SHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQAGDKAMGYS
                     HFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVAIFVAVMGA
                     SNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRYEPVI"
BASE COUNT          157 a          174 c          163 g          151 t
ORIGIN      
        1 ttcagctcaa gttcgataag aagctgacta accgttgtat cggactgacg ttacacatca
       61 gcgcctcaac cgtttttgag gttcttgccc gcttcaaagc ctcttctctg tcatggccat
      121 tacctgcgga tatatcccat gacacgctgg agaaactcat tttcccgccc aaagacacct
      181 ctgcgtcaga gcttgttatg cctgacatgc tgtacttcga tacggaaatg agaaaacctg
      241 gcgtgacgcg ccaactgttg tggatggaat acaaggcaca ggcgggtgac aaagcgatgg
      301 gatactcaca cttctgccgt tgctaccgga agtggaaaaa aacgcgacgc ctctccatgc
      361 gacaggagca tcgtgctggc gaaaagctct ttatcgactt ctgtggtcct acggtccctg
      421 ttattaatcc ggacaccgga gagatacgcc gcgtggcaat cttcgtggct gtaatggggg
      481 cgtccaacta tacctatgtt gaagcgtgcg aaggtcagga catgatgtca tggttaaatg
      541 cccacagccg ttgcctgacc ttcctgggcg gcgtaccgaa gctgctcatc cccgataacc
      601 tcagaagcgc agtgaaaaaa gccgatcgct atgagcctgt catca
//
LOCUS       DAZPAC010000582          641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZPAC010000582 DAZPAC010000000
VERSION     DAZPAC010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.598"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>641
                     /gene="fliD"
                     /locus_tag="ACHUPM_004897"
     CDS             <1..>641
                     /gene="fliD"
                     /locus_tag="ACHUPM_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR0872392.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          217 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac a
//
LOCUS       DAZPAC010000583          639 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZPAC010000583 DAZPAC010000000
VERSION     DAZPAC010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.599"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(19..369)
                     /gene="traA"
                     /locus_tag="ACHUPM_004898"
                     /pseudo
     CDS             complement(19..369)
                     /gene="traA"
                     /locus_tag="ACHUPM_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            complement(415..>639)
                     /locus_tag="ACHUPM_004899"
     CDS             complement(415..>639)
                     /locus_tag="ACHUPM_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR0872393.1"
                     /translation="DSYDNAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT          164 a          173 c          137 g          165 t
ORIGIN      
        1 acatcgtttt atttcctgtc agaatccggc aacagacata cctactgcaa taaatacaga
       61 gagtatggca aagccgacca ggaacttgac gtttttggtc atcatgtaca tgactgcacc
      121 gaccagaact tcagccagaa caacccattt aacgatactg gagtccttac cgaaggtcgc
      181 cttaaccgtt tcctgacctt tagccatcag gtccgtgcct ttaccagcag ccatagctaa
      241 ctcagggaac aacatcatca gaacagcaac cgggatcact gcacgaacca ggcgaagcat
      301 attcagacga gtgaatttag aaaaaaacga tttctttttg acgggcgcag aagtaccctg
      361 aacacttaac gctgaaccgc cccgggtttc ctggagagtg ttttatctgt gaactcaggc
      421 tgccagatca tcgtttccga tggaagcata ataagctttt tctgcttctg ccggaggagt
      481 atggcccagc ctttccagca atcgtcgatt gttataccag tccacccacg tgagtgtggc
      541 cagctccact tctgtccggt ttttccagct cttacggtgt attacctccg ctttgtaaag
      601 gcccttgatg atctctgcca tcgcgttgtc atacgagtc
//
LOCUS       DAZPAC010000584          627 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZPAC010000584 DAZPAC010000000
VERSION     DAZPAC010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.600"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..150
                     /locus_tag="ACHUPM_004900"
     CDS             <1..150
                     /locus_tag="ACHUPM_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872394.1"
                     /translation="FDNSLEKDENSSDNCFEVEKYNAYEAYNSENLYFTREEYTYDYD
                     LLNAI"
BASE COUNT          197 a          143 c          120 g          167 t
ORIGIN      
        1 tttgataata gcctcgagaa agacgaaaat tcttcggaca attgctttga ggtcgaaaaa
       61 tataacgcat atgaagcata taactcagag aatctatatt ttaccagaga ggaatatacg
      121 tatgattacg accttttaaa tgcaatatag acataataac ttcttacaaa atctataatt
      181 aataccgtag atattcattc atcttaccct gtgcaccaaa agcaaaaact cactgacgcg
      241 ggtttgaaac tgatggcaac cgcaaaaatg cctgatgcgc tacgcttatc aggcctacgc
      301 catctctaca atatattgaa tttgcgtgct tttgtaggca ggataaggcg ttcacgcagc
      361 atccggcatg aataaagcgc actcaacaat ctgaaacccg ccggaacggt ttattacgta
      421 catcaggtga aactgaccga taagccgctt tcttttgggt atagtgtcgt ggacagtcat
      481 tcatctttct gcccctccaa aagcaaaaac ccgccgaagc gggtttttac gtaaatcagg
      541 tgaaactgac cgataagccg ctttcttttg ggtatagcgt cgtggacagt cattcatctt
      601 tctgcccctc caaaagcaaa aacccgc
//
LOCUS       DAZPAC010000585          624 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZPAC010000585 DAZPAC010000000
VERSION     DAZPAC010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.601"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>624)
                     /locus_tag="ACHUPM_004901"
     CDS             complement(<1..>624)
                     /locus_tag="ACHUPM_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872395.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     PPAYRVLTVVVDGFGRSLTFHRAAEGDVAGAVTGVTDGAGRRFHMALSTQAQRAEASR
                     KQRASSLSSPASPRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTA
                     PLARYTYTAGGELRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPES"
BASE COUNT          109 a          227 c          191 g           97 t
ORIGIN      
        1 gctctccggc ctccccgcat aatggtgcgc caccatccgc ccggcgtgct ccgcatcata
       61 agtaaacccg cgaacctgcg tcccgctgcg gtcatacacc gcccgcagtt caccaccggc
      121 cgtgtacgtg tagcgcgcca gcggcgcagt cggctgttca tccgggtatg ccgggtcgtg
      181 tgtcagccat accgcctcca gccggatacc gttatcggca ccgtactctg taccggcggg
      241 cagcgtgtcg gggaaaacct gagaagagga aacagaacgg ggactggccg gggaagataa
      301 ggaagaggcg cgctgtttac gggacgcttc cgcccgctgc gcctgcgtgc tcagcgccat
      361 gtgaaaacgg cggcccgcgc cgtccgtcac ccccgtcacc gcgcccgcca cgtcaccctc
      421 cgccgcccgg tggaaagtca gtgagcgccc gaagccatcc accacgaccg tcagtacccg
      481 gtatgcgggc ggctccggcc agctgagtat ccaccacggc ccctgaaggc tgtttgtcgc
      541 caggtacaca tgcggactca ggcgaacatc ttccggcagc acctgccaga gcgcgcttag
      601 cggctgcgaa ctgtgctgcg ccgc
//
LOCUS       DAZPAC010000586          620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZPAC010000586 DAZPAC010000000
VERSION     DAZPAC010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.602"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(269..>620)
                     /locus_tag="ACHUPM_004902"
     CDS             complement(269..>620)
                     /locus_tag="ACHUPM_004902"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872396.1"
                     /translation="YIPSLMARLREVLNAIFSAPRSGKSRIPKYQQGIFLRNQHRFYL
                     LNHYTESLSVMSLTKLSLNTRMIGFMSDQMLETAPRLTRAVSDETSVYAGAGQNIGQN
                     PFNIINVICAKEHI"
BASE COUNT          194 a          128 c          110 g          188 t
ORIGIN      
        1 gcctggcata gcaagccctt ttcttgtgtt gtcaagacat ttggcaacgt tcaaacatct
       61 gtttagaaat gccaaattgt tgatctaaca aataaacctg tataaacaga acaccattta
      121 atctattccc ctgtgtaacc caccacatca cacaataaaa acaaaaaact ttaattatat
      181 ttcatagcac acaaaagtca atagaagccc tttcgtgctc ctacgtaaaa aatccatcac
      241 ttttccactg atacatcaat tcaaggcgtt atatgtgttc cttagcacat ataacattta
      301 ttatgttaaa tggattttgg cctatatttt gacctgcacc cgcataaaca ctggtttcat
      361 cgcttacagc ccttgtgaga cgtggtgctg tttctagcat ctgatccgac ataaaaccga
      421 tcattctcgt gtttagtgaa agtttagtga gagacattac agaaaggctt tctgtgtaat
      481 gatttaatag gtaaaaacga tgttgattac gcaagaaaat tccttgctga tatttgggga
      541 ttctggattt tccagatcgg ggggcgctga aaatcgcgtt aagcacctct cgcaatctag
      601 ccataaggga agggatatag
//
LOCUS       DAZPAC010000587          614 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZPAC010000587 DAZPAC010000000
VERSION     DAZPAC010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.603"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(76..423)
                     /gene="tnpB"
                     /locus_tag="ACHUPM_004903"
     CDS             complement(76..423)
                     /gene="tnpB"
                     /locus_tag="ACHUPM_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0872397.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(420..>614)
                     /locus_tag="ACHUPM_004904"
     CDS             complement(420..>614)
                     /locus_tag="ACHUPM_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0872398.1"
                     /translation="LPSSESPQLLPVTLDAAAEQPESLAEDPKTLSISCEVTFRHGTL
                     RFNGNVSEKLLTLLIQELKR"
BASE COUNT          136 a          165 c          175 g          138 t
ORIGIN      
        1 atgtcgtcag aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa
       61 ccaggataaa gagatttaca gcatggtcag ggaggtcagc agccgcttag gctgtcgcca
      121 gtcgatacct tccagcagca tcgccagctg cgcctgtgta aggaacactt tgccatcacg
      181 ggctgacggc caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc
      241 actggtggac cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaacatg
      301 gccggacatg ggatcgtctt tcagcgccgt ctgtactttc gcagccaggc cgttgaagcc
      361 atttctcata tcggtgatac cggcaaccag ccaaattttg atcccggaag gtaacgggat
      421 catcgcttca gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag
      481 cggagcgtcc cgtgccggaa cgttacctca cagctgatac tgagggtttt cgggtcctct
      541 gcgagcgatt ctggctgttc ggcagctgca tcgagagtca caggaagtag ctgggggctc
      601 tctgaagaag gtaa
//
LOCUS       DAZPAC010000588          607 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZPAC010000588 DAZPAC010000000
VERSION     DAZPAC010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.604"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>607)
                     /locus_tag="ACHUPM_004905"
     CDS             complement(<1..>607)
                     /locus_tag="ACHUPM_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR0872399.1"
                     /translation="RPSIRDSDDEELVANILGYIFLDDKPTSGSTSLDTFYGEGSTSH
                     AIHTRTQLENYIQTNGADKIVNNYLFVYEMIQKLFDANNLNFRSHILGNASSSQECPR
                     YYQAVFLALYELIINENMQLDDEQKFIAQLGDSVQRSMVQTEGGRWAASARQKSVEDL
                     CALIRRYFKESENKFINHAWQTLIRTLLNNSRTEQPNYDFK"
BASE COUNT          178 a          111 c          105 g          213 t
ORIGIN      
        1 tgtttaaaat cataatttgg ttgttctgtt cttgagttat taagtagagt tcttatcagt
       61 gtttgccaag catgattgat aaatttattt tctgattctt taaaatatcg tctgattaaa
      121 gcacataaat cttcgacact tttctgacga gcgctagctg cccaacggcc cccttcggtt
      181 tgcaccattg aacgttgtac actgtctcct aactgagcga taaatttctg ttcatcgtca
      241 agttgcatgt tttcatttat gattaattca tacaaagcaa gaaaaacagc ttgatagtaa
      301 cgaggacatt cttgagatga agatgcattg cccagaatgt gtgatctaaa gttcaaatta
      361 tttgcatcaa ataatttttg aatcatctca taaacgaaga ggtaattatt gacaatttta
      421 tctgcaccat ttgtctgaat gtaattctct aattgagttc gtgtatggat tgcatgactc
      481 gtcgaacctt caccgtaaaa tgtatcgagc gatgttgatc ctgatgttgg cttatcatct
      541 aaaaatatat agcccaagat atttgcaact aactcttcat cgtcagagtc tctaatgcta
      601 ggcctac
//
LOCUS       DAZPAC010000589          605 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZPAC010000589 DAZPAC010000000
VERSION     DAZPAC010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.605"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>605)
                     /gene="ospC2"
                     /locus_tag="ACHUPM_004906"
     CDS             complement(<1..>605)
                     /gene="ospC2"
                     /locus_tag="ACHUPM_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFR0872400.1"
                     /translation="KALQKNISCVTNSSSSGISEKHLDHCADTVKSFLRKSIAAQSYS
                     KMFSQGTSFKSLNLSIEAPSGARSSFRSLEHLDKVSRHYLSEIIQKTHPLSSDERHLL
                     SIIINSDFNFRHQSNANLSNNTLNIKSFDKIKSENIQTYKNTFSEDIEEIANHDFVFF
                     GVEISNHQETLPLNKTHHTVDFGANAYIIDHDSPYGYMTLT"
BASE COUNT          190 a           87 c          117 g          211 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatctacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt tttttgtaat
      601 gcttt
//
LOCUS       DAZPAC010000590          604 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZPAC010000590 DAZPAC010000000
VERSION     DAZPAC010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.606"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>604
                     /locus_tag="ACHUPM_004907"
     CDS             <1..>604
                     /locus_tag="ACHUPM_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR0872401.1"
                     /translation="IGAPTGVACSVCPGGVTSGHPVNPLLGAKVLPGETDIALPAPLP
                     FILSRTYSSYRTKTPAPVGSLGPGWKMPADIRLQLRDNTLILSDNGGRSLYFEHLFPG
                     EDGYSRSESLWLVRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLG
                     WCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHREAAG"
BASE COUNT          100 a          199 c          198 g          107 t
ORIGIN      
        1 atcggtgccc ccaccggtgt ggcctgttcg gtgtgccccg gcggagtgac gtccggccat
       61 ccggtcaatc ccctgctcgg tgcaaaggtc cttcccggtg aaaccgacat cgccctgccc
      121 gccccgctgc cgttcatcct ctcccgcacc tacagcagtt accggacaaa aacgcccgcg
      181 ccggtgggga gcctcggccc cggctggaaa atgcctgcgg atatccgctt acagctgcgc
      241 gataacacac tgatactcag tgataacggc ggcagaagcc tgtattttga gcacctgttt
      301 cccggtgagg acggttacag ccgcagcgaa tcactctggc tggtgcgcgg cggcgtggcg
      361 aaactggatg aaggtcaccg gctggccgca ctctggcagg cgctgccgga agaactccgc
      421 ttaagtccgc atcgttatct ggcgacaaac agtccgcagg ggccgtggtg gctgctcggc
      481 tggtgtgagc gggtgccgga agcggatgag gtgctgcctg cgccgctgcc gccgtaccgg
      541 gtactgaccg ggctggtgga ccgcttcggg cgcacacaga cgttccaccg cgaagccgcc
      601 ggtg
//
LOCUS       DAZPAC010000591          602 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZPAC010000591 DAZPAC010000000
VERSION     DAZPAC010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.607"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..498)
                     /locus_tag="ACHUPM_004908"
     CDS             complement(<1..498)
                     /locus_tag="ACHUPM_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="SpvC; OpsF from Shigella was shown to remove
                     phosphate from threonine groups; may inactivate mitogen
                     activated kinases during infection; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector phosphothreonine lyase"
                     /protein_id="HFR0872402.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPNYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQY"
BASE COUNT          193 a          116 c           96 g          197 t
ORIGIN      
        1 atattgcgag cattcctgat ctgattttac atatagcgta aactgagctc ctatccccac
       61 acgagattgc tgagaaactc gattcatatc agttatcttc catttatcta taggactgtc
      121 ctctgaaaat aataaaccag acaaaatttg gaatgccaga ggaacttgct ccctagctat
      181 actaatatga aacttgtccc caacaaaatc ccccttactc tgaggatttt cacgacatgc
      241 atgaataaac acatctccac tatggttcac aatctggaat ccttggtaca tgacagaaaa
      301 aaattgacat atatcagagt aatttggcac gttcgatgct actttgaaat agtacgctgg
      361 gtattgccgt atttggttgt acaatatatt gaagttattt ttcaatcttt cattcgcaga
      421 aagcatctgc ggtatttcag acctcacaac attcaaagaa tctaaattta gttttagaca
      481 gggctttttt atgggcatag aaaacgtcct ctataaaata gatatattat cttatattga
      541 ttgtctatca tcttttataa aacgatacat tatatgtaat gtatgtaagc gtaagggtct
      601 tc
//
LOCUS       DAZPAC010000592          593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZPAC010000592 DAZPAC010000000
VERSION     DAZPAC010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.609"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..91
                     /locus_tag="ACHUPM_004909"
     CDS             <1..91
                     /locus_tag="ACHUPM_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872403.1"
                     /translation="GLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            289..>593
                     /gene="mcbR"
                     /locus_tag="ACHUPM_004910"
     CDS             289..>593
                     /gene="mcbR"
                     /locus_tag="ACHUPM_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR0872404.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          172 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tga
//
LOCUS       DAZPAC010000593          590 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZPAC010000593 DAZPAC010000000
VERSION     DAZPAC010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.610"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..245
                     /gene="ospE2"
                     /locus_tag="ACHUPM_004911"
     CDS             <1..245
                     /gene="ospE2"
                     /locus_tag="ACHUPM_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HFR0872405.1"
                     /translation="CLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAAIYELQIPA
                     ANRTKTLKFTATEMQWLTKINEVGIVEKQSQRHSNI"
BASE COUNT          208 a          108 c          102 g          172 t
ORIGIN      
        1 cttgccttcc acaaaaacaa gaaaatatca tccttgaagt atcaaatcct gtattattat
       61 cttctactgt tactacagat ggatatactg ttttcaataa aaaagcagct atatacgaat
      121 tacaaatacc tgctgccaac cggacgaaaa cgttgaaatt cactgctaca gaaatgcaat
      181 ggttgacaaa gataaatgaa gtaggaattg ttgaaaaaca aagccaacgg cattctaata
      241 tctgaagtta atttcagact cgtttaagct ccattttcaa aagaatatat cagggtttgt
      301 tactagtaat ttctggaaac agaaaaagta gagcagtaat tctatttcag agaggagtaa
      361 ctccatctat cctcgataca tactgaacgt tatcgtgata tattcatcat gctttcttca
      421 ccattattcg agtgcaggga cgcaatgatc ttaaatacct tttagtagaa tatattgctg
      481 acccacaaat atcaggatgg tattgacaag tcatagatgg agaaatatat gactgaatta
      541 ccattctctg gaataacagc agactccttc ccatagacgg cttagggggg
//
LOCUS       DAZPAC010000594          582 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZPAC010000594 DAZPAC010000000
VERSION     DAZPAC010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.611"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..190
                     /locus_tag="ACHUPM_004912"
     CDS             <1..190
                     /locus_tag="ACHUPM_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="SpvC; OpsF from Shigella was shown to remove
                     phosphate from threonine groups; may inactivate mitogen
                     activated kinases during infection; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III effector phosphothreonine lyase"
                     /protein_id="HFR0872406.1"
                     /translation="IMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDRDGS
                     ERQEQMLREEPFYRLMIE"
BASE COUNT          156 a          124 c          126 g          176 t
ORIGIN      
        1 tataatgtgt ctcgagtcta atctattaag aagcaaaatc gctcccgggg agtatccggc
       61 gtcagatgtt agacctgaag actggaaata tgtcagctat cgtaatgaac tacgaagcga
      121 tcgagatgga agtgaaaggc aagagcaaat gttacgagag gaaccatttt atcgtttgat
      181 gatagagtag agaagatgcc agctacacca ccttgtagct tgaaaactgg ttagaataac
      241 tacgattaga tttggcactg cgtatgcagc ctgattcctg cccgtgacag agcattcggc
      301 tcataaactg ctgagctact catctgaaat ccgaacagtt gtccaccact tcagcgtgca
      361 tctcctgttt gagtcgtttg attgcttttc gggatattaa cctgtcggat tttgcattag
      421 ttaatgctcc catcaggcct tcatcttttc cagtgcctat tttcctgttc tccttttgca
      481 caatataatc agatcatctg tatcgttgac gagctcgcta tcttatagga gcattctgaa
      541 taagctacct ccttgtaagg aggcttttac tattagtaac tg
//
LOCUS       DAZPAC010000595          578 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZPAC010000595 DAZPAC010000000
VERSION     DAZPAC010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.612"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..271
                     /locus_tag="ACHUPM_004913"
     CDS             <1..271
                     /locus_tag="ACHUPM_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872407.1"
                     /translation="TEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRL
                     QDQQEETSVTLFQGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
BASE COUNT          173 a          139 c          119 g          147 t
ORIGIN      
        1 aacagaggcc accctgtacg aaaacgggct gtatacgcgc cacatcagac tcagtcgccc
       61 agtatctgac agcaaatacc ccttgccacc cggatctcag ctcattaaaa atatgacctt
      121 acgactgcgc ctgcaggatc agcaggaaga aacatccgtg acattatttc agggaaaaat
      181 gagtaccgac ggaaataact attacatcat caaaggtaaa gtaagctcag tacttctgtt
      241 attaaaaatg ctgcagatta atcacgcctg atctctgtcc cggtatttta gcccgaggta
      301 tattctgaaa tatcattcat catgaccggc gtttatacaa acgccggtca ttcgctaaca
      361 gcttcaccgt tccttccccc atccaggttc cgcatgagcg tgttgtaatg ttcataagcg
      421 tattatcagg attgtctgga aaattcccga tactttggac tattactcct gaacaattcg
      481 aaattcagca gattaaaggt aagcgttaac ggaggtaagc gttaacggag gtaagcgtaa
      541 accgactgcc gtatgtagcc attaagccag tattggta
//
LOCUS       DAZPAC010000596          575 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZPAC010000596 DAZPAC010000000
VERSION     DAZPAC010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.613"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>575)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUPM_004914"
     CDS             complement(<1..>575)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUPM_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR0872408.1"
                     /translation="RDEAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSY
                     NQLTNLPELPVTLKKLYSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNI
                     SYNEIVSLPSLPQALKNLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILN
                     LRNECSIHISDNPLSSHALQALQRLTSSPDY"
BASE COUNT          161 a           95 c          145 g          174 t
ORIGIN      
        1 gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg ataatgggtt
       61 atcactaata tgtattgaac attcattcct cagattaaga atgctttccg ggatatgact
      121 tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct cagaaaatgc
      181 tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct gtgggagcga
      241 tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat cgggtaaagc
      301 tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag gaggtagcac
      361 gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa caggcagttc
      421 aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag ctggtaagtt
      481 gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat tattgataag
      541 acattcttta agtcgggaga cagcctcatc acgct
//
LOCUS       DAZPAC010000597          575 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZPAC010000597 DAZPAC010000000
VERSION     DAZPAC010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.614"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
BASE COUNT          157 a          113 c           91 g          214 t
ORIGIN      
        1 tatttatact tatcgggtat cagttttgat aggggtatca gacttgatag gggtaacaga
       61 attctgtggg ggtagttcac tatgacctac ccctagttca ctgtgagcta ccgttacatc
      121 caatcatctt ccgatgtaac gtatcgttac taccgatgtg taatatcgtt acattcaata
      181 attagtctat gtaatatatc gttactacca actttcttac agagagcatt tcagataaat
      241 gaatgtaacc tctcgttaca tccatttgtc acatagcact ctgtgtaatg tctttctatg
      301 tgtgtgccat aatcccccta tattccctgt gtaaatcata gaataatgct ctctattccc
      361 tgtgtaattc cctactaaaa aatccaatga aaagtagctt aaaagttcaa ttcttacttt
      421 cttcactttt acatcacttt cgcaaagcct tgtgtaatgc ggcttacaga gcaccatcag
      481 aaattgttgg ctaaaaattg ttctgtttgt ttctgaacga gtttctcgtt tgtgcaaatt
      541 ttaaaattta ttttgttttt atagccttat ttcgt
//
LOCUS       DAZPAC010000598          574 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZPAC010000598 DAZPAC010000000
VERSION     DAZPAC010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.615"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..516)
                     /locus_tag="ACHUPM_004915"
     CDS             complement(<1..516)
                     /locus_tag="ACHUPM_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR0872409.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDSK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNP"
BASE COUNT          160 a          137 c          115 g          162 t
ORIGIN      
        1 tgggttgctg gcatcattgc ctttcgcatc acccggacag ttagaagagg ctacccacca
       61 agcattttta tataccgcac cattaaagac gacataccag gtctgaccgc cctgctgttt
      121 attccaggca actacagaag aattagacgg aacagtgccc tgtgctggtg tagccgaacc
      181 gccattagcc ggcgtattgg atgcaggcgt atttgaattt gaagaagatg agctgccgct
      241 cttttcgcag gaaagtgtat tgccaaactg actaatttca gcagctgttg cggtacgctt
      301 taaacgccac gggttagttg catcatttgc tttcgctttt cccgggcaat ttgtagaaaa
      361 accccaccag gcattttcat aaattttgct atcgaaaata acctgatatt tattaccacc
      421 ttgttggtta ttccatgctt ccattgccaa tgctggcaga gcggaacaca ccagccccat
      481 accaatcata gatttagtaa atatatttaa tttcattata atcccttgtg acgtaaaaac
      541 tgcaaaacaa aagtcctacg caggaattat ttac
//
LOCUS       DAZPAC010000599          573 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZPAC010000599 DAZPAC010000000
VERSION     DAZPAC010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.616"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(35..418)
                     /gene="traM"
                     /locus_tag="ACHUPM_004916"
     CDS             complement(35..418)
                     /gene="traM"
                     /locus_tag="ACHUPM_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFR0872410.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
BASE COUNT          175 a          138 c           84 g          176 t
ORIGIN      
        1 ctgatatttg aacaaagtca aaatttattt ccctttattc atcatcattt tttggaaaga
       61 acctttccat ctcagccgac accttctctc tgatatcttc aaccatattg gcatattcaa
      121 actttggatt tcctgagaca tgaggactga gagactcaat acccaaaatt ttcgctactg
      181 atgactgtgt ttttacaacg cattcaagaa gtagtttatt aaactcggcc tgattaaatg
      241 cagactcttt gcgctccatc tgagcctcat atacacgaag acccagttca agaagcattg
      301 aagccgttgc tgaaaaactg acatcttttt ctctcgcccc ctcctgtcga cgtttttcaa
      361 taatcgcgtt tattttttca taagcatcat tgctgatata cagattcacc ttagccatta
      421 gatattacct tttttaaaca taaaacaaag ttaatttcga aacactagaa ttcatagtgc
      481 aaacagggac gcaccgctag cagcgcccct gacggtatcc tataaaaaaa cacaccgcgc
      541 cgctagcagc acccctaata taaaataatg ttt
//
LOCUS       DAZPAC010000600          570 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZPAC010000600 DAZPAC010000000
VERSION     DAZPAC010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.617"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..439
                     /locus_tag="ACHUPM_004917"
     CDS             <1..439
                     /locus_tag="ACHUPM_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR0872411.1"
                     /translation="DIEKQLTSVARQNETCKRLLDIPGVGPLIATAAVATMGEASAFK
                     SGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTLFIHGARAVALVAKEPGPWIT
                     ELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHVSIRPY"
BASE COUNT          168 a          124 c          159 g          119 t
ORIGIN      
        1 ggatattgaa aaacagctta ccagcgtggc gaggcagaat gaaacctgta agcggttgct
       61 ggatattcct ggcgttggac cacttattgc gacggcagcg gtggccacca tgggggaagc
      121 atcagcgttt aaatcggggc gagagttcgc cgcatatgtt ggtctggttc caaaacaaac
      181 tggctccgga gggaaagtac gtctgctggg gataagcaaa cgtggtgaca cttatctcag
      241 gacattattt atccacggtg caagagcggt ggcattagta gctaaagagc ctggcccgtg
      301 gataaccgaa ctgaaaaaac gtcgtccagc cagtgtggca atcgtcgcca tggcaaacaa
      361 gctggcacga acagtatggg cgataaccgc ccatgaccgt aagtatgaca ggaaccacgt
      421 cagtatcaga ccatattaat cgctgatacc attaaacaat gaactcttaa caaaagggtg
      481 aatgctgaaa ggttgctatg gcggccagag tgatgacaaa gacaggtaag accgtgactc
      541 actaaacctg aacagtattt tgggcttgaa
//
LOCUS       DAZPAC010000601          563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZPAC010000601 DAZPAC010000000
VERSION     DAZPAC010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.618"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..311
                     /gene="tnpA"
                     /locus_tag="ACHUPM_004918"
                     /pseudo
     CDS             1..311
                     /gene="tnpA"
                     /locus_tag="ACHUPM_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311304.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          167 a          132 c          120 g          144 t
ORIGIN      
        1 atgacgcgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat tttgaagctg
       61 aactggttga aatggatggc gaaccagatc acgtccattt gttaattaac tatcctccca
      121 aactggcgat atccagtctg gtaaacagcc tcaaaggcgt atcgggtagg tgactgcgac
      181 gagatcgacc agatattgca gtcaggtatt actacaaagg cgttttgtgg agtgttggct
      241 attttgccag tagctgcgga ggtgcgccaa tatccgtcat ccgccaatac attgaacaac
      301 agcaaacacc tggttaggtg gaaaaccacg ccttatatcc ctgccctgaa ggacggggtt
      361 ttacggcgca ccggataagc ctggagatta tctacaccag catggacatc attgtactat
      421 gccccgtaca tgccccatca catcctcaac atcaaagtat tagagccttg caaaccatta
      481 tgcgtctcag tttgtctcat ctatcttacg caaaaaaaaa tagaactaat atcaagcggc
      541 gctatattgc gtattaaata aaa
//
LOCUS       DAZPAC010000602          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZPAC010000602 DAZPAC010000000
VERSION     DAZPAC010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.619"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>557
                     /locus_tag="ACHUPM_004919"
     CDS             <1..>557
                     /locus_tag="ACHUPM_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0872412.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLK"
BASE COUNT          115 a          160 c          157 g          125 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAZPAC010000603          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZPAC010000603 DAZPAC010000000
VERSION     DAZPAC010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.620"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>557
                     /locus_tag="ACHUPM_004920"
     CDS             <1..>557
                     /locus_tag="ACHUPM_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0872413.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLK"
BASE COUNT          111 a          162 c          159 g          125 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAZPAC010000604          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZPAC010000604 DAZPAC010000000
VERSION     DAZPAC010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.621"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>557
                     /locus_tag="ACHUPM_004921"
     CDS             <1..>557
                     /locus_tag="ACHUPM_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFR0872414.1"
                     /translation="IPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRALNARL
                     RELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPDYHVEY
                     ARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHMPERHR
                     RHGTWTPERLIEQGNRTGPSTGRVV"
BASE COUNT          142 a          169 c          148 g           98 t
ORIGIN      
        1 tatcccggcc cgcccgcgca aacctaaaga taaaccaaaa gcagaaaacg gcgtcctcat
       61 cgtcgagcga tggcttcttg cccgcatccg taatgaaacc ttccacacgc tcagggcgct
      121 gaatgcacgc cttcgggagt tgctgacgga catgaataac cgaccaatga agggatacgg
      181 taatcaaacc cgcgcagaac gcttccgcat gctggatgca ccagcactct ctccgctgcc
      241 tctggagcct tatgaataca cggaatacaa agccgtgaag gttggccctg actaccacgt
      301 tgaatatgcc cgtcactggt actccgttcc acacgaactg gtcggtcagc gtctgtcact
      361 gaaggtgggg cagtcggtgg tacagctctg gcacaagggg cagtgtgtgg cgcaacatcc
      421 gcgcagcaca catgaatata aacacacaac caatccgctt cacatgccag aacgccaccg
      481 gcggcatggg acatggacac cggaacgcct tatcgagcag ggaaaccgta caggtccgtc
      541 cacaggccgg gtggttg
//
LOCUS       DAZPAC010000605          555 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZPAC010000605 DAZPAC010000000
VERSION     DAZPAC010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.622"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>555
                     /gene="feaR"
                     /locus_tag="ACHUPM_004922"
     CDS             <1..>555
                     /gene="feaR"
                     /locus_tag="ACHUPM_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFR0872415.1"
                     /translation="QEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDASR
                     PCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNNP
                     ALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWIA
                     GETGMSVRSLYRMFADKGLVVAQY"
BASE COUNT          142 a          125 c          146 g          142 t
ORIGIN      
        1 ggcaggaagt aaaaggcagc gacgatgcct ggttttacac cgtttttcag cttagtggtc
       61 aggcaataat ggagcaggat gagcgtcagg tgcagattgg cgctggcgat attacgttac
      121 tcgatgcctc acgcccctgt tcgctttact ggcaggagtc ttctaaacag atttcattac
      181 ttttgccacg cactctgctg gaacaatatt ttccccatca aaaacctgtc tgcgcagaaa
      241 gactggacgc tgacttaccc atggtgcaac tcagtcatcg cctgttacag gagagcatga
      301 ataatccggc actttctgaa acagaaagtg aagctgcgct acaggcgatg gtgtgtctgc
      361 tgcgcccggt acttcatcag cgggaatctg ttcaacctcg ccgtgaacgt cagtttcaaa
      421 aagtggttac gttgatagac gataatattc gcgaagagat attacgcccg gagtggatag
      481 ccggagagac aggtatgtca gtacgtagtt tgtaccgaat gtttgccgat aaaggtttgg
      541 tagtcgcgca atata
//
LOCUS       DAZPAC010000606          551 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZPAC010000606 DAZPAC010000000
VERSION     DAZPAC010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.623"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            28..>551
                     /locus_tag="ACHUPM_004923"
     CDS             28..>551
                     /locus_tag="ACHUPM_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0872416.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQR"
BASE COUNT          172 a          102 c          113 g          164 t
ORIGIN      
        1 gaaacaatat tgtaagatca aatttatatg gtggcgaaaa ttaatctctt atcacggctt
       61 acgccgcttt tattcgtgtt cgctcctttt ttggcacagt caaatatgac tgtttatcct
      121 atggctgttt ccattaatag tcagggtgaa ggcaacgttc gcgtaatatc taaaagtaat
      181 gaagttcagt acatcaaggc gacagtattc cgtatcgata atccatcgac gcctcaggaa
      241 aatgaagttg agattaaatc cggtgatgca aatcatctgg ttgttatgcc acctaaattc
      301 gctttaccag ccggtagtag caaaactgtg cgctttgttg caatggaacc agagcaaaaa
      361 gaaaaaaatt atcgcgttaa atttgaagcg gttcccagta ttgatgacgt tgccacagat
      421 aaaaaagatc tctctatgca gttaacagtt aacttaattt gggggattgt tgttagtgtt
      481 ccacctcagc aacctattgc taagttagaa gtaaatgctg cccaaaaatt agttaatgca
      541 gggaatcaac g
//
LOCUS       DAZPAC010000607          549 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZPAC010000607 DAZPAC010000000
VERSION     DAZPAC010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.624"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>549
                     /locus_tag="ACHUPM_004924"
     CDS             <1..>549
                     /locus_tag="ACHUPM_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR0872417.1"
                     /translation="VVISKYTDHLPLYRQQHIFSRMGVELPVSTMADMVGVAGAALAP
                     LVKLLRHELLTRDVIHADETSLRLLDTRKGGKSCSGWLCAYVSGERSGPPVVCFDSQT
                     GRALRYPETWLQCWCGGTLVSDGYSVYKSLADNHPGITSACCWSHAGRGFANLYKASR
                     EPRAGVELRKIAGLYRIEKLIRE"
BASE COUNT          113 a          150 c          166 g          120 t
ORIGIN      
        1 gttgtcatca gcaaatatac cgaccatctg cccctgtatc gccagcagca cattttctcc
       61 cgaatgggtg tggagctccc cgtcagtaca atggcggata tggtcggtgt tgccggagct
      121 gcactggccc cgctggtgaa actgctacgc catgagcttc tgacacggga tgttattcat
      181 gcagatgaaa cgtccctgag gctccttgat acccggaaag gcggtaaatc ctgctccggc
      241 tggctgtgtg cttacgtcag cggtgaaaga agcggtccgc cggtcgtctg ttttgactcg
      301 cagaccggac gggccctcag ataccctgag acctggctgc agtgctggtg tggcggtacg
      361 ctggtgagtg acggatacag cgtgtacaaa tctctggcgg ataatcaccc cggcatcaca
      421 tcagcctgtt gctggagtca cgcagggcgc ggcttcgcaa atctgtataa ggccagcagg
      481 gagccccgtg caggcgtgga gctgagaaaa atcgcgggtc tttaccgcat tgagaaactc
      541 atccgtgag
//
LOCUS       DAZPAC010000608          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZPAC010000608 DAZPAC010000000
VERSION     DAZPAC010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.625"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHUPM_004925"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHUPM_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          194 a          120 c           83 g          149 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggc
//
LOCUS       DAZPAC010000609          541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZPAC010000609 DAZPAC010000000
VERSION     DAZPAC010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.626"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..159
                     /locus_tag="ACHUPM_004926"
     CDS             <1..159
                     /locus_tag="ACHUPM_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0872418.1"
                     /translation="RDAYCNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIR
                     LFYQRLRN"
     gene            298..453
                     /locus_tag="ACHUPM_004927"
                     /pseudo
     CDS             298..453
                     /locus_tag="ACHUPM_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          152 a          116 c          131 g          142 t
ORIGIN      
        1 cgtgatgcgt actgcaatcg taatgtcgtt gaacggtgct ttgggcggct gaaagaatat
       61 cgccgcatcg ccacgcgtta cgacaaaacg gcgagaaatt acctggcgat ggtgaaactg
      121 ggctgcatcc gactctttta tcaacgctta cgtaattaag ggacacagcc tggcactctg
      181 cgagtaaact tgctggtaaa ctattattga cagttggttt tgttttgaga ctgtaattta
      241 aattgtgtaa ttgcctgttt ttgatatgtt cactccaaca acggagacag gtaaattatg
      301 gacgaaaaga agcttaaagc ccttgcggct gaactggcta aaggcctcaa aaccgaggcc
      361 gacctcaatc agttttcccg tatgcagacg aaattaactg tcgaaacggt gcttaatgcg
      421 gaactaactg accatctcgg gcatgaaaaa agctacatcc ggtagtaaac attatcagtg
      481 ggttatgcga tatgatttga gccgttttca gacaatcgca gggccagtac ctcgtcagtc
      541 a
//
LOCUS       DAZPAC010000610          541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZPAC010000610 DAZPAC010000000
VERSION     DAZPAC010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.627"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..409
                     /locus_tag="ACHUPM_004928"
     CDS             <1..409
                     /locus_tag="ACHUPM_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR0872419.1"
                     /translation="ADIHGRVVRVLDGDTIEVMDSLKAVRIRLVNIDAPEKKQDYGRW
                     STDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTD
                     PVLPVLQNEARQQKRGLWSDADPVPPWIWMHRK"
     gene            455..>541
                     /locus_tag="ACHUPM_004929"
     CDS             455..>541
                     /locus_tag="ACHUPM_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="with Hns involved in transcriptional regulation of
                     hemolysin; non-specific DNA-binding protein which affects
                     the production of multiple proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0872420.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNR"
BASE COUNT          161 a          112 c          141 g          127 t
ORIGIN      
        1 tgcagatatt catggacggg ttgttcgtgt actggacggt gataccatcg aggtcatgga
       61 ctcactgaag gcggtgcgga tcaggctggt aaatattgat gcaccggaaa agaaacagga
      121 ttatggccgg tggtcaacag acatgatgaa atcactggtc gcaggaaaaa cggttaccgt
      181 cacatatttt cagcgagatc gttatggtcg tattctcggg caggtttatg caccagacgg
      241 aatgaatatt aatcagttta tggtccgggc cggggctgcc tgggtatatg agcagtacaa
      301 cacagatccc gtgctacctg tccttcagaa cgaagccaga caacaaaaac gtgggctctg
      361 gtcagatgct gatccggttc ccccctggat atggatgcac cgtaaataaa gcgatggcaa
      421 tcctatcatt catctgttct cagcgggaat aaaaatggca aaaacaaaac aggaatggct
      481 gtatcagtta cggcgatgtt catcagtcaa tacgctggaa aaaattattc ataaaaacag
      541 a
//
LOCUS       DAZPAC010000611          539 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZPAC010000611 DAZPAC010000000
VERSION     DAZPAC010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.628"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            128..539
                     /locus_tag="ACHUPM_004930"
                     /pseudo
     CDS             128..539
                     /locus_tag="ACHUPM_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          146 a          135 c          124 g          134 t
ORIGIN      
        1 aactctaaac atgagaaggt ttttaacgta aggtgagagg atttttaagt gtatccatag
       61 attaaaaatg acaatgcctc caattagttg aattaggggt tataataccc gcttttaagg
      121 ttctttcatg gtcacagtta accttcattg cccccgttgc cagtccgttc aggtttaccg
      181 tcacgggcag aacccaaaag gacatgatcg attccgttgc cgtgactgtc accgggtgtt
      241 tcagctcact tactgttacg aggcccgtaa accgggagtt aaaaaccaaa tcactgaaat
      301 ggctttcaac ggtgccggag ttcgcgatac tgcccgtacg ctgaaaatcg gcatcaatac
      361 cgtcatacgg acattaaaaa gctcccgccc cggcggataa catctgcgcc agtcgctcac
      421 gctgatgttg cccttatctg tgaacttgat gggcagtgga gcttcgttgg cagcaaggcg
      481 caacaacact gtcacgaacg gtgcaatagt gatccacacc caacgcctga aatcagatc
//
LOCUS       DAZPAC010000612          539 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZPAC010000612 DAZPAC010000000
VERSION     DAZPAC010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.629"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            93..>539
                     /locus_tag="ACHUPM_004931"
     CDS             93..>539
                     /locus_tag="ACHUPM_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR0872421.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT          120 a          119 c          136 g          164 t
ORIGIN      
        1 gatatacgct atgcgaaaat gcagatggca atgagatcca ctgctttcat ctccattaac
       61 atcccattac gcttttatta aggagcatta gcatgttctc accgcagtca cgcttgcgtc
      121 atgcagttgc agatacgttc gcgatggttg tttactgttc tgtcgtgaac atgtgtattg
      181 aagttttcct ctccggaatg agcttcgaac agtcttttta ttccagattg gtagcgattc
      241 cggtgaacat cttaattgca tggccatacg gtatgtaccg cgatctattt atgcgcgcgg
      301 cacgcaaagt tagcccgtcg ggctggataa aaaatctggc tgatatcctg gcttatgtga
      361 cgttccagtc accggtgtat gtggcgatct tgttagtggt gggcgcagac tggcatcaga
      421 ttatggcggc ggtcagttca aacatcgttg tttcgatgtt gatgggggcg gtttatggct
      481 acttcctcga ttattgccgc cgactgttta aagtcagcca ttaccagcag gtaaaagcc
//
LOCUS       DAZPAC010000613          530 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZPAC010000613 DAZPAC010000000
VERSION     DAZPAC010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.630"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(23..358)
                     /locus_tag="ACHUPM_004932"
     CDS             complement(23..358)
                     /locus_tag="ACHUPM_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR0872422.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT          137 a          121 c          116 g          156 t
ORIGIN      
        1 cggcttaccg atcacaatac aatcagttct ttttcacaaa ctcagatttc agtttcatcg
       61 gaccaaaacc gtcgattttg caatcgatgt tatggtcgcc ttcaaccagg cggatgtttt
      121 tcactttggt gccaattttc agcatcgaag agctaccttt caccttcaga tctttaatga
      181 tggtaacgct gtcgccgtca gccagcagat tgccgttagc atctttaacg atcagctcgt
      241 cgctttcctg tgcaggttct gcgtcgttcc attcgtaggc acattccggg cagatgtaca
      301 tgccgttatc ttcgtaagtg tattcggagt tgcattttgg gcagtgtggt aatgacatgt
      361 ggatttcctc aaaagtcagc agggcaaaag cgccaaaaaa cggcagattg ccgaaaaagg
      421 ccgcaattat acacaaaatc cctgagtttg tcgggggtac tgctgatttt agacgacgat
      481 cgttttcatt tgaaaggaaa cgcaaaaaga aaataatctt tttgcctcta
//
LOCUS       DAZPAC010000614          528 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZPAC010000614 DAZPAC010000000
VERSION     DAZPAC010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.631"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            12..>528
                     /locus_tag="ACHUPM_004933"
     CDS             12..>528
                     /locus_tag="ACHUPM_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HFR0872423.1"
                     /translation="MPVTKCEPETTRKASRKYAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQR"
BASE COUNT          119 a          144 c          161 g          104 t
ORIGIN      
        1 aggggatgat tatgcctgta acgaagtgtg aaccagaaac cacccgcaaa gcaagccgta
       61 aatatgcaaa aacgcaggaa acagtcctgt ctgccctgct ggcgcagacg gaggaagtga
      121 gcgtgccgct ggcctcgctg attaaatcac cgctgaatgt gcgcacggtg ccgtattctg
      181 cggagtccgt cagcgaactg gcggagtcca ttaagggcgt tggcctgttg cagaatctgg
      241 tcgtgcatac cctgcctggt gaccgttacg gtgtcgccgc aggtggtcgc cgactggctg
      301 cactcaacat gctggcagag cgtggcatca ttccggctga ctggcctgtc cgtgtgaagg
      361 tcattccgca ggagctggcg acggcggcat caatgactga aaacggtcat cgtcgggata
      421 tgcaccctgc tgaacagatt gccggattcc gcgcaatggc gcaggaaggc aaaacacctg
      481 cacaaatcgg tgatttgctg ggctattcac cccgccacgt tcagcgaa
//
LOCUS       DAZPAC010000615          523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZPAC010000615 DAZPAC010000000
VERSION     DAZPAC010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.632"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>523
                     /gene="icsB"
                     /locus_tag="ACHUPM_004934"
     CDS             <1..>523
                     /gene="icsB"
                     /locus_tag="ACHUPM_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR0872424.1"
                     /translation="NLFGLDETNMSKFICQKKADAFRQLANYKLISKSENCAGMALNV
                     LKAGNSEIYFPLPDVKLVATPNNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRL
                     TQLRRSYLKSFNKINLIHTPKTFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSA
                     PSNMSRVLNELKTG"
BASE COUNT          188 a          103 c           76 g          156 t
ORIGIN      
        1 aacctttttg gccttgatga aacaaacatg tcaaaattta tatgccaaaa gaaagcagat
       61 gctttcagac aattggcaaa ttataaatta atcagtaaat ctgaaaactg tgctggtatg
      121 gcacttaatg ttcttaaagc aggaaattct gaaatatact ttccactccc tgacgttaaa
      181 ttggttgcta cacctaacaa tgtatacgcg tatgcaaata aagttaggca acgcattgaa
      241 agtctcaatc agtcttataa cgagataatg aagtatattg aatctgattt tgatctttcc
      301 agattgactc aattacgacg tagctatctt aaaagtttca ataaaattaa ccttatccac
      361 accccaaaaa cattcaagcc tttatcaatt tcactttaca aacatcctac tgaaaatgta
      421 tcttcagaag actttgatgc ggtcatcaac gcttgccact catacttagt aaaatcagca
      481 cccagcaata tgtctcgtgt attaaacgaa ttaaaaaccg gag
//
LOCUS       DAZPAC010000616          520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZPAC010000616 DAZPAC010000000
VERSION     DAZPAC010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.633"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>520
                     /locus_tag="ACHUPM_004935"
     CDS             <1..>520
                     /locus_tag="ACHUPM_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR0872425.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLA"
BASE COUNT          138 a          121 c          117 g          144 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc
//
LOCUS       DAZPAC010000617          517 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZPAC010000617 DAZPAC010000000
VERSION     DAZPAC010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.634"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..465
                     /gene="pagP"
                     /locus_tag="ACHUPM_004936"
     CDS             <1..465
                     /gene="pagP"
                     /locus_tag="ACHUPM_004936"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR0872426.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          144 a          113 c          126 g          134 t
ORIGIN      
        1 acgtttagag aaaatattgc acaaacctgg caacagcctg aacattatga tttatatatt
       61 cctgccatca cctggcatgc acgtttcgct tacgacaaag aaaaaaccga tcgctataac
      121 gagcgaccgt ggggtggcgg ttttggtcag tcgcgatggg atgaaaaagg aaactggcat
      181 ggcctgtatg ccatggcatt taaggactcg tggaacaaat gggaaccgat tgccggatac
      241 ggatgggaaa gtacctggcg accgctggcg gatgaaaatt ttcatttagg tctgggattc
      301 accgctggcg taacggcacg cgataactgg aattacatcc ctctcccggt tctactgcca
      361 ttggcctccg tgggttatgg cccagtgact tttcagatga cctacattcc gggtacctac
      421 aacaatggca atgtgtactt tgcctggatg cgctttcagt tttgagacaa atgaagtttt
      481 agtaacttct ttaaaatcaa taactaaaat aagtaac
//
LOCUS       DAZPAC010000618          510 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZPAC010000618 DAZPAC010000000
VERSION     DAZPAC010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.635"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHUPM_004937"
     CDS             <1..173
                     /locus_tag="ACHUPM_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0872427.1"
                     /translation="VIHRKSWKNRAEVELAILTWVDWYNNRRLLERLGHTPPAEAEKA
                     YYASIGNDDLAA"
     gene            215..415
                     /locus_tag="ACHUPM_004938"
                     /pseudo
     CDS             215..415
                     /locus_tag="ACHUPM_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          159 a          117 c          124 g          110 t
ORIGIN      
        1 aggtaataca ccgtaagagc tggaaaaacc gtgcagaagt ggaactggcc atactcacgt
       61 gggtggactg gtataacaat cgacgattgc tggaaaggct gggccatact cctccggcag
      121 aagcagaaaa agcttattat gcttccatcg gaaacgatga tctggcagcc tgagttcaca
      181 gataaaacac tctccaggaa acccggggcg gttcaacctt cacgataaaa aacttttcat
      241 taaaggtaaa gcggatcaat atccggcact tcattctctg atatctccga cccgcattaa
      301 tctgaaagaa atagaaattc actggcgtga agtgctacgt ctggcgacct caataaaaca
      361 gggaacagtg acagcatccc tgatgctgag aaaacttgcc agttacccca aacaaaagta
      421 aggcagtatg actttccgtg agacagatgc tggccaacgt gggatattta acgcgggtta
      481 acatcgttgt cagagccgca tgggtgagct
//
LOCUS       DAZPAC010000619          509 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZPAC010000619 DAZPAC010000000
VERSION     DAZPAC010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.636"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>509)
                     /gene="espL1"
                     /locus_tag="ACHUPM_004939"
     CDS             complement(<1..>509)
                     /gene="espL1"
                     /locus_tag="ACHUPM_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFR0872428.1"
                     /translation="LDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALLRHPPA
                     GSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSVYGQLNYVV
                     SVYDPNDTNVAVRGTHRAARGFLSLDKFISSAPDAQTWADRYVRNCAIAILPLLPEGV
                     PVAILAGITT"
BASE COUNT          149 a          115 c          117 g          128 t
ORIGIN      
        1 gtcgtgatac ccgctaaaat agcaactgga actccctcag gtaatagagg aagaatagca
       61 attgcacagt tgcgaacata cctatcagcc caggtctgag catcgggagc tgaactgatg
      121 aacttatcaa gcgagagaaa gccccgtgct gccctgtggg tgcctctgac ggcaacgttg
      181 gtatcgttcg ggtcgtaaac agacacaaca taatttaatt gtccatacac tgactgcttt
      241 attcgtagcc ttactgacaa agtatgattg tctatatata aaatcgcgga catatgactg
      301 acattgtttg ccatcattcg acaaaagaaa atatctaaag cgaagccaaa atccttactc
      361 gcgataacgc gactccctgc aggaggatgg cgaagtaatg ctctgaactc cgcgtcccgg
      421 actaagggga catgctgctg tatggattca acggaggagt agtgtttaat caggttgact
      481 ttgccttttc catttctcaa tgaatcaag
//
LOCUS       DAZPAC010000620          507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZPAC010000620 DAZPAC010000000
VERSION     DAZPAC010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.637"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..188)
                     /locus_tag="ACHUPM_004940"
     CDS             complement(<1..188)
                     /locus_tag="ACHUPM_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0872429.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDG"
     gene            242..445
                     /locus_tag="ACHUPM_004941"
                     /pseudo
     CDS             242..445
                     /locus_tag="ACHUPM_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          126 a          136 c          117 g          128 t
ORIGIN      
        1 ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc ggacacgcag agtctccggc
       61 gtacagccaa tctttggggc aatggaacaa attgccgccc actgtgagtc atattcatcc
      121 tgactttcca gaaccatacg aatcgcccgc tgacggactt cgggggaaaa acgagtattt
      181 ttagtcatcc tgtttacctc tttctcaggg agtttagtct ccaggattcc cggggcggtt
      241 cagacatcta gaatttactg tcagcaccgg cgttaaagtt tacttctgcg atcctcagag
      301 tccttggcag cggggaacaa atgagaatac aaatgggcta attcggcagt actttcctaa
      361 aaagacatgt cttgcccaat atactcaaca tcgagtaggc agcctgacgg ctgcggcttg
      421 tcatggtctg caattaccgt tataaaaaca ggcaatgtca ttgtctttca ggtggttata
      481 tggcccgttc agctaaaccc cgtaaac
//
LOCUS       DAZPAC010000621          507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZPAC010000621 DAZPAC010000000
VERSION     DAZPAC010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.638"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            71..146
                     /locus_tag="ACHUPM_004942"
     tRNA            71..146
                     /locus_tag="ACHUPM_004942"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105..107,aa:Glu,seq:ttc)
     gene            340..>507
                     /locus_tag="ACHUPM_004943"
     rRNA            340..>507
                     /locus_tag="ACHUPM_004943"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          141 a          110 c          129 g          127 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgata gaaagtgaaa agcaaggcgt cttgcgaagc
       61 agactgatac gtccccttcg tctagaggcc caggacaccg ccctttcacg gcggtaacag
      121 gggttcgaat cccctagggg acgccacttg ctggtttgtg agtgaaagtc acctgcctta
      181 atatctcaaa actcatcttc gggtgatgtt tgagatattt gctctttaaa aatctggatc
      241 aagctgaaaa ttgaaacact gaacaacgaa agttgttcgt gagtctctcg aattttcgca
      301 acacgatgat gaatcgaaag aaacatcttc gggttgtgag gttaagcgac taagcgtaca
      361 cggtggatgc cctggcagtc agaggcgatg aaggacgtgc taatctgcga taagcgtcgg
      421 taaggtgata tgaaccgtta taaccggcga tttccgaatg gggaaaccca gtgtgtttcg
      481 acacactatc attaactgaa tccatag
//
LOCUS       DAZPAC010000622          504 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZPAC010000622 DAZPAC010000000
VERSION     DAZPAC010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.639"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..194
                     /locus_tag="ACHUPM_004944"
     CDS             <1..194
                     /locus_tag="ACHUPM_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0872430.1"
                     /translation="LKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKIV
                     VKYNNWITKDNGEFELAVH"
     gene            224..>504
                     /locus_tag="ACHUPM_004945"
     CDS             224..>504
                     /locus_tag="ACHUPM_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR0872431.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSL"
BASE COUNT          172 a           96 c          107 g          129 t
ORIGIN      
        1 gcttaaagat tttaacgatc gcttattgta aaaataatag caaagaaaat tgtaagatac
       61 agaccgtaaa taaaaacatc ttccttggtc aggaaagaaa tcttgaaagc atatctggct
      121 acgacaaaat cgttgtcaaa tataataact ggatcaccaa agataatggc gagtttgaac
      181 tggcggtcca ttaattatca ttgtaataaa cgaaggaata gtaatgaaaa aggtatttgc
      241 aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc tccgcattgg cagtacaaaa
      301 ggatattact gtaacggcca acgttgatgc cgctctggat atgacacaga ccgataacac
      361 cgcgttgccg aaagcagtag aaatgcaata tctgccgggg cagggtctgc aatcttacca
      421 gttgatgacc aaaatctggt ctaacgacgt taccaaagat gtaaaaatgc agttagtttc
      481 cccggcgcaa ctggttcaaa gcct
//
LOCUS       DAZPAC010000623          503 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZPAC010000623 DAZPAC010000000
VERSION     DAZPAC010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.640"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..503
                     /locus_tag="ACHUPM_004946"
                     /pseudo
     CDS             1..503
                     /locus_tag="ACHUPM_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT          123 a          124 c          140 g          116 t
ORIGIN      
        1 ggccggacgc ccatgggctg agcaccgtaa aatcatcaat ggcatgttct gggtgttatg
       61 ttccggtgca ccatggcgcg atttacccga acgatatggg tcatggaaaa ctgtttataa
      121 ccgctttaac cgatggtcaa agtcaggcgt gattaatatt attttcaaca ggttgctttc
      181 gctacttgat gcaaacggct ttattgactg gtctgccacc gcgctggatg gcagtaatat
      241 ccgggcgctg aaatgtgccg ccggtgctca aaaaaacatc ccgatatcga cggagataat
      301 gggccgggtc gctctcgcgg cggttttggc accaaaatcc atctggcaac agacggaagt
      361 ggcctcccgt taaatatcgt gctaagcccc ggacaagctc atgaaagcca gttcgcgcaa
      421 cgacttctgg acgggattgg cgttcagcgt cagaacggca gcatgaaacg ccgtggacat
      481 gcggtactgg ctgataaagc gta
//
LOCUS       DAZPAC010000624          497 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZPAC010000624 DAZPAC010000000
VERSION     DAZPAC010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.641"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            47..>497
                     /locus_tag="ACHUPM_004947"
     CDS             47..>497
                     /locus_tag="ACHUPM_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HFR0872432.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLK"
BASE COUNT          119 a          119 c          130 g          129 t
ORIGIN      
        1 gggtgagctg ttgctacacc ccggtatttc caccacggag gccagcatga acaacaataa
       61 cacattgtac gtcgggttag atgttcacaa agaatcgatt actgtcgctt atgctatcaa
      121 ttcagaacct gttgaactga tgggtaaaat tggcacatca cctactgata ttcagaatct
      181 ttgtaaacgt ctcaggtcaa agtcatcgca ggtcagtatc gtttatgaag cggggccctg
      241 tggttatgga ctttatcgtc ggctggtgaa atccggtttt gactgcatgg tctgcgcccc
      301 ctcacttatc ccgaaaaaac cgggggagcg ggttaaaacc gatcgccgtg atgccatcag
      361 acttgtgcgt tcactgcgtg caggagatct ttctgctgtc tacgtacccg gcattgaaga
      421 tgaggcattc cgggatttgg cccgggcatg ggcatctgcc cgcgatgatt tacggcatgc
      481 aaggcaacgt ctgaaat
//
LOCUS       DAZPAC010000625          490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZPAC010000625 DAZPAC010000000
VERSION     DAZPAC010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.642"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(234..446)
                     /locus_tag="ACHUPM_004948"
     CDS             complement(234..446)
                     /locus_tag="ACHUPM_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFR0872433.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
BASE COUNT          169 a           86 c           70 g          165 t
ORIGIN      
        1 tgattcattg ttcccaaaat gatgttttcc ccggccccgc caataatgtg cagctctcac
       61 gtgataatct attgattagg ttatattaag aatcaatatt taaaatacat ccattttctt
      121 gaataaatca acatatttat ttatcaattg ctgttttagc tctgactcat caagctctcc
      181 atatttatcc tatagataat tgcgaataaa tacagagaac gccatttatc cttttagcat
      241 acctgccgga tatttttatt tgcattaata ttaattgcat ctccaactcc gagcatatcc
      301 atcattaaaa actttttgag attatagaat acctgtgata acaagtgaaa catctgcaca
      361 atgtgaatag ttagtttctt ttcagaggaa atgaaataga caagctgact gaaggggaga
      421 gtgatataaa tcaaaaaaca taacatgaat ttaattaatt acgcccatca ttgtggataa
      481 agttactgaa
//
LOCUS       DAZPAC010000626          489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZPAC010000626 DAZPAC010000000
VERSION     DAZPAC010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.643"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
BASE COUNT          145 a           96 c           96 g          152 t
ORIGIN      
        1 tccattttac ccagagtcat aaaatagagt gtctgttgat ctcattttca tattcctttc
       61 agactgaagt gcgttttcat cacccgatga gaaagaactg aagtgatgtt tgccatagat
      121 tgcgagatgc cctgtctcat tgactatcgc ggtgcacctt tatacctgtt ataccagatc
      181 aaaaatcacg caagccatac aacaaaacga gatttgcaat tcgtgtcaca aaatatgtcg
      241 atctttttct aagaggaaga tgccatgtga agccagacga acacttgagg tggtcttcaa
      301 aaactaaagg atttctttta actgtttgtt ttataaaaaa tttatttagg gtctaaacaa
      361 gggaagcttt gcaagctaac tcagtgagcg tggtgaaaac aagtgtttac ccgccatcag
      421 gctgatcata attctcacca tgaaatatgt ttcctgattt gtggcttgta actggtcgct
      481 tctgaagtc
//
LOCUS       DAZPAC010000627          482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZPAC010000627 DAZPAC010000000
VERSION     DAZPAC010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.644"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            362..>482
                     /locus_tag="ACHUPM_004949"
     CDS             362..>482
                     /locus_tag="ACHUPM_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0872434.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISG"
BASE COUNT          170 a           68 c          100 g          144 t
ORIGIN      
        1 aagtgtgaat ggcattaacg tgtgttttga aaaaaatgaa atgataggtt ggattaaaaa
       61 agaaatccct ttgctgtcgc aaaaaatagg tgggctttaa atggtagggc aatattcaaa
      121 gacagtatag gtaactattt gtatggccat gtaaggtaaa agaaaaggaa taaaccatga
      181 gcggaaaacc ggcggcgcgg cagggcgaca tgacccgcag ggaaagatca tcagaagttc
      241 actttttgta ctaaataatt cgcattttat gtttaaaaat tgagatattc cttattacct
      301 aaagctgttt tttattgctt atacatgatc aaatacttct tacataatta aggagaacaa
      361 aatggaactt aaaaaattga tggaacatat ttctattatc cccgattaca gacaagcctg
      421 gaaagtggaa cataaattat cggatattct actgttgact atttgtgccg ttatttctgg
      481 tg
//
LOCUS       DAZPAC010000628          482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZPAC010000628 DAZPAC010000000
VERSION     DAZPAC010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.645"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            41..>482
                     /gene="mxiJ"
                     /locus_tag="ACHUPM_004950"
     CDS             41..>482
                     /gene="mxiJ"
                     /locus_tag="ACHUPM_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFR0872435.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHI"
BASE COUNT          150 a           69 c          104 g          159 t
ORIGIN      
        1 cagtttcggc tgttgaaact ttattaaagt cttaatttat atgattaggt ataaaggttt
       61 tattttattc ttgttgctga tgttgattgg atgtgagcaa cgtgaagagt taatttctaa
      121 tttatctcaa agacaggcaa atgaaataat atctgtgcta gaacgccata atattactgc
      181 tagaaaagtt gatggaggta aacaggggat ctcggtacaa gtcgaaaagg ggacatttgc
      241 atcggcagtt gatttgatgc gcatgtacga tttgccaaat ccggagagag ttgatatctc
      301 acaaatgttt cctacagatt cattagtgtc ttctccaaga gctgaaaagg cccgtttata
      361 tagtgctatt gagcaacggc tggaacagtc tttagtttct attggtggtg ttatttcggc
      421 aaaaatacat gttagctatg atcttgaaga aaaaaatata tcttcaaaac cgatgcatat
      481 at
//
LOCUS       DAZPAC010000629          480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZPAC010000629 DAZPAC010000000
VERSION     DAZPAC010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.646"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            129..>480
                     /locus_tag="ACHUPM_004951"
     CDS             129..>480
                     /locus_tag="ACHUPM_004951"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HFR0872436.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIV"
BASE COUNT          113 a          113 c          128 g          126 t
ORIGIN      
        1 cactttccgc aaattatctc gccattaacc gtttcagcct caggtgcctt tcttgaggca
       61 atcgcctgtt ggtggtatcg tttatcgctt tttcaaaaaa atcgacacat tttaagggga
      121 ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc ggggaaaggg actcaggctc
      181 agttcatcat ggagaaatat ggtattccgc aaatctccac tggcgatatg ctgcgtgctg
      241 cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga cattatggat gctggcaaac
      301 tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg cattgctcag gaagactgcc
      361 gtaatggttt cctgttggac ggcttcccgc gtaccattcc gcaggcagac gcgatgaaag
      421 aagcgggcat caatgttgat tacgttctgg aattcgacgt accggacgaa ctgattgttg
//
LOCUS       DAZPAC010000630          478 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZPAC010000630 DAZPAC010000000
VERSION     DAZPAC010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.647"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
BASE COUNT          148 a           96 c           88 g          146 t
ORIGIN      
        1 cttctgggga acgctgaaaa atgaaagcct gagccactat cgttttaaga gccgggatat
       61 ttcgtcagca tacgggaaaa ctgattgagg tcggcctcag ttttgaggcc tttagccagt
      121 tcagccgcaa gggctttaag cttcttttcg tccataattt acctgtctcc gttgttggag
      181 taaacatatc aaaaacaggc aattacacaa tttaaattac agtctcaaaa caaacccaac
      241 ggtcaatata gtttaccagc aagcttacta gcaaagtgct aatcatttgg tatctttcgt
      301 gctattctcc ttttacgtaa gtacttaagg ggaacgtatt tactactttt aaagaaagct
      361 ccgttactat ctgaaatgca atgagataat aaacgattac tctcggttag tatctcatga
      421 tcatttacag actttgcata atcgcgaata ctatataaca gagttttaac attacagg
//
LOCUS       DAZPAC010000631          476 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZPAC010000631 DAZPAC010000000
VERSION     DAZPAC010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.648"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>476
                     /locus_tag="ACHUPM_004952"
     rRNA            <1..>476
                     /locus_tag="ACHUPM_004952"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZPAC010000632          473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZPAC010000632 DAZPAC010000000
VERSION     DAZPAC010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.649"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>473)
                     /locus_tag="ACHUPM_004953"
     CDS             complement(<1..>473)
                     /locus_tag="ACHUPM_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR0872437.1"
                     /translation="VYTFILSVLIYREVKWRHLPKLILESVVTTSIVLLLIGFSVGMS
                     WAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMTPAVLIFTPIFLPIA
                     QELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQHLIKPLLPFYAS"
BASE COUNT          162 a          107 c           98 g          106 t
ORIGIN      
        1 tgctagcata aaatggcagc aatggtttga ttaaatgttg aatcttcaca cccgagatag
       61 aacaaccaac aaatagcgca ctacccaccg gcggtgttaa cagcccaata caaaggttgg
      121 ccaccatcat aataccgaaa tgtactgggt ccattcccag ctcttgtgca atcggcaaga
      181 agatcggtgt aaagattagt acagccggtg tcatgtccat aaagatgccg acaattaata
      241 agacgatatt aatcagcagc aatatgatca gtgggttatc agaaatcccc atcaatgcgt
      301 cgctgatcat ataaggaata tcagcattag tcattgccca ggacatgccc acggaaaaac
      361 cgatcaacag taatacaatc gaagtggtaa caaccgactc cagaatcagt ttgggtaaat
      421 ggcgccattt tacttcccgg taaatcagca ccgagagaat aaatgtgtag aca
//
LOCUS       DAZPAC010000633          472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZPAC010000633 DAZPAC010000000
VERSION     DAZPAC010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.650"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            89..448
                     /locus_tag="ACHUPM_004954"
                     /pseudo
     CDS             89..448
                     /locus_tag="ACHUPM_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT          114 a          112 c          138 g          108 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt gggtacgtcc tgaaagactg aggtagcctg agtttaacgg ac
//
LOCUS       DAZPAC010000634          471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZPAC010000634 DAZPAC010000000
VERSION     DAZPAC010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.651"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..286
                     /gene="traL"
                     /locus_tag="ACHUPM_004955"
     CDS             <1..286
                     /gene="traL"
                     /locus_tag="ACHUPM_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR0872438.1"
                     /translation="KYRFPETLTNQSRWFGLPLDELIPAAICVGWGITTSKYLFGIGA
                     AVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWIK"
     gene            308..>471
                     /locus_tag="ACHUPM_004956"
     CDS             308..>471
                     /locus_tag="ACHUPM_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HFR0872439.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGF"
BASE COUNT          113 a          106 c          115 g          137 t
ORIGIN      
        1 gaaatatcgt ttcccggaaa cactgaccaa ccagagccgc tggtttggcc tgccactgga
       61 tgaactgatc cccgcagcaa tctgtgttgg ctggggtatc acaacatcga aatatctgtt
      121 cggtattggt gcagcggttc tggtttattt cgggattaaa aaactgaaaa aagggcgggg
      181 cagttcctgg ttacgtgacc tgatttactg gtatatgcca acagccctgc tgcgcggtat
      241 ttttcataat gttcccgatt cgtgtttccg gcagtggatt aaatagaact gataccagga
      301 ttgttatatg gaacacggtg cccgtttaag taccagtcgt gtaatggcca tcgcctttat
      361 atttatgtca gtgcttattg ttctcagcct ctctgttaac ctcattcagg gggtgaataa
      421 ctaccgtctt cagaatgagc aacgcactgc cgtgacccca atgggattta a
//
LOCUS       DAZPAC010000635          471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZPAC010000635 DAZPAC010000000
VERSION     DAZPAC010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.652"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..471
                     /locus_tag="ACHUPM_004957"
                     /pseudo
     CDS             1..471
                     /locus_tag="ACHUPM_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT          167 a           59 c           83 g          162 t
ORIGIN      
        1 gacaggttta aacagagata actttattta tatcagtgat atatttttaa atataaagca
       61 acggaacgaa aaaatcatgc aataaatatg ttacgtgaag tctcaattag taatgatact
      121 ataagtgtaa aattctacag aaatgaaaaa atagaatgcg cttgtaattt tctgatggat
      181 aaagatgcgc aggggtatat cgacctgtct gattttgatt taataaattg tcattttaaa
      241 ggtgacgtta tttctaaggt gtctttttta tcatcaaatc tacaacatgt aactttcgaa
      301 tgtaaagaaa ttgagaattg caattttact aaggcaacag ttgataatgt catatttaaa
      361 tgtcgacgtt tacacaatgt gatttttctc aaaacgagtg gcgaatgtgt tgattttagc
      421 caaaatattc ttgatacagt tgacttctcg cagagtcaac ttggtcatag t
//
LOCUS       DAZPAC010000636          470 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZPAC010000636 DAZPAC010000000
VERSION     DAZPAC010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.653"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
BASE COUNT          162 a           90 c           80 g          138 t
ORIGIN      
        1 tttttatgcg gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt
       61 agcattagaa tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt
      121 ccttacaaat agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact
      181 gtactcgtca ctacacgcac atacaacgga ggggggctgc gattttcaat aatgtgtgat
      241 gcagatcaca caaaacactc aattacttaa cataaatgta taagtcatac ttttgttttg
      301 ggtgtatttc aatatgttaa aaagtttttc gctacgctag caagcaaaaa tgaaacagga
      361 ataatcgaaa tgggatgttg cgcacagtta aaataaatca ccgtaaataa tcatctgcta
      421 taaataatca ctttcatgca ataccagata aactattttt aaacagacac
//
LOCUS       DAZPAC010000637          464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZPAC010000637 DAZPAC010000000
VERSION     DAZPAC010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.655"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(234..>464)
                     /gene="tuf"
                     /locus_tag="ACHUPM_004958"
     CDS             complement(234..>464)
                     /gene="tuf"
                     /locus_tag="ACHUPM_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR0872440.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
BASE COUNT          157 a           96 c           97 g          114 t
ORIGIN      
        1 gttttacgag aacccttcaa tatgaattaa agtgaggcat taaaaataag aaaaaagcct
       61 gacaaatgaa gaattttaaa aacagaaaca ttcatattta aaatattaaa ttgaattgat
      121 attttaaata tgaataattt attcgttctg acagtacgaa taagatatgc agtcaacaaa
      181 tgcaaaaagg gcgccgaagc gcccttttca attcaaaact aattaacgtg taattagccc
      241 agaactttag caacaacgcc cgcgccaacg gtacggccgc cttcacggat tgcgaaacgc
      301 agaccgtcgt ccatcgcgat cgggtggatc agggtaacaa ccattttgat gttgtcgccc
      361 ggcattacca tctctacgcc ttccggcagt tcgatggtac cagtcacgtc agtagtacgg
      421 aagtagaact gcggacggta gcctttgaag aacggagtat gacg
//
LOCUS       DAZPAC010000638          461 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZPAC010000638 DAZPAC010000000
VERSION     DAZPAC010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.656"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>461
                     /locus_tag="ACHUPM_004959"
     CDS             <1..>461
                     /locus_tag="ACHUPM_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0872441.1"
                     /translation="LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTL
                     RSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQ
                     GASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          134 a           94 c          143 g           90 t
ORIGIN      
        1 tctgctgacg tcgatgacgg acgccatgcg cttccccgga ggagaaatgg cggatctgta
       61 cagtcatcgc tgggaaatcg aactgggata cagggagata aaacagacga tgcaactgag
      121 caggctgacg ctgagaagta aaaagccgga gcttgtggag caagagctgt ggggtgtctt
      181 actggcttat aatctggtga gatatcagat gattaaaatg gcggaacatc tgaaaggtta
      241 ctggccgaat caactgagtt tctcagaatc atgcggaatg gtgatgagaa tgctgatgac
      301 attgcagggc gcttcaccgg gacgtatacc ggagctgatg cgcgatcttg caagtatggg
      361 acaacttgtg aaattaccga cgagaaggga aagggccttc ccgagagtgg taaaggagag
      421 gccctggaaa taccccacag ccccgaaaaa gagccagtca g
//
LOCUS       DAZPAC010000639          459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZPAC010000639 DAZPAC010000000
VERSION     DAZPAC010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.657"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..77
                     /locus_tag="ACHUPM_004960"
     CDS             <1..77
                     /locus_tag="ACHUPM_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR0872442.1"
                     /translation="AARLDKIRRQNRIKAELQAVLDEK"
     gene            207..419
                     /locus_tag="ACHUPM_004961"
     CDS             207..419
                     /locus_tag="ACHUPM_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR0872443.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT          140 a          105 c          138 g           76 t
ORIGIN      
        1 cggcagcgcg tctggataaa atccgccgcc agaaccggat aaaggcagaa cttcaggccg
       61 tgcttgatga gaaataaaaa aaagcctcca tcagagagga ggcagggaaa taaggcttaa
      121 aaggaaataa cttccgcttc cgaaaggaag ccagtccact gaattattta tcacaataat
      181 actgacaact aaagggggac aagaaaatga atggattcag aaacagttca cggaacggcc
      241 aggtctggcg ttaccagcgt gccggcgggc gggccgttat tctggaagtc agtggacgct
      301 ggatggaagc ggcagaagca tggcgacggg ctgcctgtgt tgcgcccagg acagactggc
      361 agcagtttgc ccgaaaaagg gctgaacact gccaccggcg ctgccgggga agggtgtaac
      421 gaaaaaagca gccttcatta ctccaggagt cagaaggct
//
LOCUS       DAZPAC010000640          458 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZPAC010000640 DAZPAC010000000
VERSION     DAZPAC010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.658"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..244
                     /locus_tag="ACHUPM_004962"
     CDS             <1..244
                     /locus_tag="ACHUPM_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shufflon protein C"
                     /protein_id="HFR0872444.1"
                     /translation="APKIQFTTQTYNIAKNTRNLRLGVHAYCSWTYLNGSPFGGFQQV
                     YSDQNNVWYVSNYAWGNYESGGTISVTCLNLPGAGA"
     gene            complement(241..>458)
                     /locus_tag="ACHUPM_004963"
     CDS             complement(241..>458)
                     /locus_tag="ACHUPM_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Shufflon protein B"
                     /protein_id="HFR0872445.1"
                     /translation="WSGGNKINYSACNWYKSSVAMNHFIGGKSGGSIYYKPIQCPTGY
                     IMTGTRMYGIGDGVDEEHVDAYCCPFN"
BASE COUNT          131 a          110 c           88 g          129 t
ORIGIN      
        1 cgctcctaaa attcaattca caacgcagac ctacaacata gctaaaaaca ctcgtaattt
       61 aaggctcggg gtccatgcat attgctcatg gacttacctg aatggctcac cctttggtgg
      121 ttttcaacag gtatattccg accaaaacaa cgtttggtat gtgagtaatt atgcttgggg
      181 aaattatgag tctggtggga ccatatcagt cacatgcctc aatcttcctg gtgctggagc
      241 ttaattaaag gggcaacagt aggcatcgac atgttcctca tcaacaccat caccaatacc
      301 gtacatgcgt gttccagtca taatgtatcc agtcggacat tgaatcggct tgtaatagat
      361 cgaaccacca ctcttaccgc cgataaagtg attcattgct acggatgatt tgtaccaatt
      421 acatgctgag taattaattt tattaccccc tgaccacc
//
LOCUS       DAZPAC010000641          455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZPAC010000641 DAZPAC010000000
VERSION     DAZPAC010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.659"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>455)
                     /locus_tag="ACHUPM_004964"
     CDS             complement(<1..>455)
                     /locus_tag="ACHUPM_004964"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFR0872446.1"
                     /translation="VAGLGLLFLFPVFIIVSMLIVADSKGGVFFRQYRVGRFGKDFRI
                     HKFRTMFIDSEKKGRITVGQDARVTRVGWYLRKYKIDELPQLIDVLSGTMSLVGPRPE
                     VREFIDEYPDDIREKVLSVRPGITDLASIEMVDENEILSSYDDPRRAYI"
BASE COUNT          148 a          120 c           54 g          133 t
ORIGIN      
        1 tctatataag ccctacgtgg gtcatcataa ctagacaaaa tctcattttc atctaccatt
       61 tctatagatg ctaagtcagt tatccctggc ctaaccgata aaactttttc ccttatatca
      121 tcaggatact catcaataaa ctccctcact tccggtcttg ggccaaccaa actcattgtt
      181 ccagaaagaa catctatcaa ttgaggaagc tcatcgattt tgtacttccg taaataccat
      241 ccaactctgg ttacccgagc atcttgacca actgttatcc gtcctttttt ttctgaatcg
      301 ataaacatcg ttctaaattt atgtatccta aaatctttcc caaatctccc aactctatac
      361 tgcctaaaaa aaaccccccc tttagaatca gcaacaatta acattgacac aatgataaaa
      421 acaggaaata gaaaaagcag gcctaagcct gccac
//
LOCUS       DAZPAC010000642          454 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZPAC010000642 DAZPAC010000000
VERSION     DAZPAC010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.660"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>454
                     /locus_tag="ACHUPM_004965"
     CDS             <1..>454
                     /locus_tag="ACHUPM_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872447.1"
                     /translation="IYERAEIAFDDDIDFYLKNGYLSTESNIRGIIEEKNKQYKEHKA
                     LCDVNNVWDIFSDSFKDNESEFISKIKCVINDNLSHIPVAHFIGLIDILNRLKIDCDS
                     YIEAYADAFVSQDDAYTAFRNLYVEIFGNEKLGLLIQKKLMDKKPEETN"
BASE COUNT          161 a           54 c           87 g          152 t
ORIGIN      
        1 atttatgaac gagcggaaat tgctttcgat gatgacatag atttttatct caagaatggt
       61 tatctttcta ctgaaagcaa tattcgaggt ataatagaag aaaaaaataa acaatataaa
      121 gaacacaagg ctttatgtga tgttaacaat gtttgggata tttttagtga ctcattcaag
      181 gacaatgaat ctgagtttat ttctaagata aaatgtgtaa ttaacgacaa tctttcacac
      241 atacctgttg ctcacttcat aggcttgatt gatatcttaa atagattgaa gattgattgt
      301 gatagttata tagaggcata tgcagatgcc tttgttagcc aagatgatgc ttatactgct
      361 tttcggaatt tgtatgtaga gatatttggt aacgaaaagt taggtctgct aattcaaaaa
      421 aaattgatgg ataagaagcc tgaagagact aatt
//
LOCUS       DAZPAC010000643          454 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZPAC010000643 DAZPAC010000000
VERSION     DAZPAC010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.661"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..386
                     /locus_tag="ACHUPM_004966"
     CDS             <1..386
                     /locus_tag="ACHUPM_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0872448.1"
                     /translation="KGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDVY
                     NSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNIIC
                     SECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          133 a           95 c          122 g          104 t
ORIGIN      
        1 ttaaagggca ggttgttgaa cttgtacgtc tgagcggtcg cgagacgttg cggcaactgg
       61 aagccaagac aggtgcgaca agatatctga tgagtgttct cgccagagag ctggttgcca
      121 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      181 ggcaaaacgc ccgcaaaaaa ctctcaaggg caaatctgaa gaaaccatct gtggttgatc
      241 ctgaccttat ctggtcatta ccagacggag aaatacgccg ctacgacagg cgtcaaaaca
      301 taatctgtag cgagtgccgg aagagcgaag ctatgcagcg tgtactggct ttctatcagg
      361 gtaattttca gaaggtgctg ttgtgagcca aattaacaat cggaacttcg tgaagagaaa
      421 gcataatcca aaatctgaat aattaagttc agca
//
LOCUS       DAZPAC010000644          453 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZPAC010000644 DAZPAC010000000
VERSION     DAZPAC010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.662"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(370..>453)
                     /locus_tag="ACHUPM_004967"
     CDS             complement(370..>453)
                     /locus_tag="ACHUPM_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872449.1"
                     /translation="AGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          135 a           92 c           64 g          162 t
ORIGIN      
        1 gggcagagtt tttgcgggga agtattttgt tggtaaaaat attatcaact tcatctgagt
       61 gagaattatt ctactatttc ccgagctgca ttcatttttt gtatttttta tttttagaaa
      121 ataacagttc tcaaagctta attacctcac tattaattgt atttcaaact aacaatcgca
      181 ctccattttc tctttttaaa aaacataaac actgttaaaa caaatactta tttcgttatc
      241 ttaaatgcta tttctacatc actcgtcctt ataatcaaat tattcttatt catagctatt
      301 tttgcaggtt taaaatagcg gctttcacca aagtcagcca tgaaaattag actacaagtc
      361 agggcaagat tacgaaagcc cgctccccgc aaggactgac gccagatagt ttctgtccat
      421 ggctgctttt cgcatcttac gtcttaaccc tgc
//
LOCUS       DAZPAC010000645          453 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZPAC010000645 DAZPAC010000000
VERSION     DAZPAC010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.663"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>453
                     /locus_tag="ACHUPM_004968"
     CDS             <1..>453
                     /locus_tag="ACHUPM_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0872450.1"
                     /translation="QRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFNSE
                     TVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRSPE
                     SNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALG"
BASE COUNT          111 a          108 c          148 g           86 t
ORIGIN      
        1 tcagcgatgg tgctctgacg ggttcgagtt ccgctgtgat aacggagaaa aactgcgagt
       61 cacgttcgcg ctggactgct gtgaccgtga ggcactgcac tgggcggtca ctacgggcgg
      121 cttcaacagt gaaacagtac aggacgtcat gctgggagcg gtggaacgcc gcttcggcaa
      181 cgagcttccg gcgtctccag tagagtggct gacggataat ggttcatgct accgggctaa
      241 tgaaacacgg cagtttgccc ggatgttggg gcttgaaccg aagagcacgg cggtgcggag
      301 tccggagagt aacggcatag cagagagctt cgtgaaaacg ataaagcgtg actacatcag
      361 tgtcatgccc aaaccagacg ggttaacggc agcaaagaac cttgcagagg cgttcgagca
      421 ttataacgaa tggcatccgc atagtgcact ggg
//
LOCUS       DAZPAC010000646          450 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZPAC010000646 DAZPAC010000000
VERSION     DAZPAC010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.664"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..348)
                     /locus_tag="ACHUPM_004969"
     CDS             complement(<1..348)
                     /locus_tag="ACHUPM_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0872451.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFG"
BASE COUNT          157 a           91 c           97 g          105 t
ORIGIN      
        1 accaaacttc ggatcgtttc cgtaacgatt gctgccagaa gtaccttctg tgcagaaaaa
       61 aaccagaagc acgagccagc caataaacgg aacaaaaaag agcaaggccc aggcacctga
      121 acggtcggta tcgtgtaagc gacggatagc aagcgcaagg acgggaagga aggttgccag
      181 caaataaagc atagacagat atggaagctc cagacctaaa atcaattgaa tgacattgat
      241 aatagcgccg acgatggcgt taatcagagt aaacatccag tactctttac gacgtgcgcg
      301 accagagaaa ccaacataat tttttaatac ggataaatac cactgcataa aaacatccct
      361 ttaattaaaa gtgacgaaag tctattccgg gtattgtgta aatgctattc gtatctttct
      421 ttttcggatg ttataaaggt tttgatattt
//
LOCUS       DAZPAC010000647          444 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZPAC010000647 DAZPAC010000000
VERSION     DAZPAC010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.665"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            126..401
                     /locus_tag="ACHUPM_004970"
     CDS             126..401
                     /locus_tag="ACHUPM_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFR0872452.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT          112 a          101 c           91 g          140 t
ORIGIN      
        1 aagttatgaa tgcggtgact gaataaagct atttttattc atcgagaaaa tagccatact
       61 gacgccttca atggatattg atattcacta cagatattgt tcgcagcaga caggatgtat
      121 ttaatatgcc aacagttctc tctcgcatgg cgatgcaact caaaaaaaca gcctggataa
      181 ttcccgtctt catggtttcg ggatgctcat tatctccggc aatcccggtg atcggtgctt
      241 attatcccag ctggtttttc tgcgccattg ccagccttat tttgatgctc atcacgaggc
      301 gagttattca gcgggcaaat atcaatctgg catttgtcgg aattatttat accgcccttt
      361 ttgctctcta cgccatgctg ttctggctgg catttttcta atctatttga gacgcgctca
      421 tggaaagtac gccgaaaaaa gctc
//
LOCUS       DAZPAC010000648          444 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZPAC010000648 DAZPAC010000000
VERSION     DAZPAC010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.666"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            36..>444
                     /locus_tag="ACHUPM_004971"
     CDS             36..>444
                     /locus_tag="ACHUPM_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR0872453.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGD"
BASE COUNT          130 a           77 c          109 g          128 t
ORIGIN      
        1 aatacgttta acgataatta aaaggattct gttaaatgaa attaaaaata gttgcggtgg
       61 ttgtaactgg tttgttagct gcgaacgtag cacacgctgc cgaagtctat aacaaggatg
      121 gtaataaact cgacctttat ggcaaggtta ccgctctacg ttattttact gatgataagc
      181 gtgacgatgg tgataaaact tatgcccgtc tcggctttaa aggagaaacg caaatcaatg
      241 atcaaatgat tggttttggt cactgggaat atgattttaa aggctataac gatgaagcca
      301 acggctcgcg cggcaacaag acccgtctgg cctatgctgg tttaaaaatt agtgaatttg
      361 gctctctgga ctatggtcgt aactacggtg tcggctacga cattggttca tggaccgata
      421 tgttgccaga atttggtggc gata
//
LOCUS       DAZPAC010000649          444 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZPAC010000649 DAZPAC010000000
VERSION     DAZPAC010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.667"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..438
                     /locus_tag="ACHUPM_004972"
     CDS             <1..438
                     /locus_tag="ACHUPM_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0872454.1"
                     /translation="TDNKKTETDAIYDKVRSSYLLSCILKKNKNVGLILHAPSFVSVS
                     EKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFVHKIKLTEAIRKL
                     RRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          154 a           72 c           80 g          138 t
ORIGIN      
        1 actgataata aaaagacgga gacagatgcc atttacgata aagttcggag ttcttatttg
       61 ctttcatgta ttctgaaaaa aaacaaaaat gttgggctta ttcttcatgc accatcattt
      121 gtttctgtgt ctgaaaaaat cgccagaatt gtgatggcaa attactcccg taactggagt
      181 aattccgagt tggccagtgc ggttttaatg agtgaatctt ctttaaaaag aagaatgtac
      241 aaagaggtcg gttctataag tacatttgta cataaaataa agcttacaga agctatcagg
      301 aaattacgaa ggacaaatac tcccatttct gtcatatcat ctgagttagg ttacagttca
      361 ccatcgtatt tttctaaagt tttctttaaa tatttaaaga cataccctca aaacataagg
      421 aagaaaaatg gtcggtaatg tcac
//
LOCUS       DAZPAC010000650          444 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZPAC010000650 DAZPAC010000000
VERSION     DAZPAC010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.668"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>444
                     /locus_tag="ACHUPM_004973"
     rRNA            <1..>444
                     /locus_tag="ACHUPM_004973"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          123 a           90 c          139 g           92 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggca
//
LOCUS       DAZPAC010000651          442 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZPAC010000651 DAZPAC010000000
VERSION     DAZPAC010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.669"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>442
                     /locus_tag="ACHUPM_004974"
     CDS             <1..>442
                     /locus_tag="ACHUPM_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR0872455.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL"
BASE COUNT           94 a          127 c          153 g           68 t
ORIGIN      
        1 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
       61 ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga acgggacctg
      121 acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg ggacggcgac
      181 cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca gccggggagc
      241 ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac gcagcgccgc
      301 agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      361 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct gg
//
LOCUS       DAZPAC010000652          441 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZPAC010000652 DAZPAC010000000
VERSION     DAZPAC010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.670"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..120)
                     /locus_tag="ACHUPM_004975"
     CDS             complement(<1..120)
                     /locus_tag="ACHUPM_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872456.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEI"
     gene            complement(376..>441)
                     /locus_tag="ACHUPM_004976"
     CDS             complement(376..>441)
                     /locus_tag="ACHUPM_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFR0872457.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          128 a           90 c           87 g          136 t
ORIGIN      
        1 aatttctgca aactttcttt ttaccgtcca ataatcaata tttctattgt gattgttaac
       61 cactgcgtgc attggcggga ttgaagggtg aaaaatatgt gcaattggga agcctttcat
      121 tgatgcctgc cttgtcgtca tgacaaacaa taatagatgc ttattttcct cgcagggttc
      181 tttcgcacta tcaaaaaacg ctcatttacc cggcgttaca gtaaacgaag caacattctg
      241 tggcctacga cgatcaaaag ctctgtgaaa atttcccttg ttgtctttgt ctgaccttat
      301 cgcggcgatc agcaattatt cgttttgtga tcctcaacac aaaatgctat tcgtcgccta
      361 tgaggggaga agaatttatg gaaaacgatt gcattcacaa aatgacgtcc tgtcagaaga
      421 gaaaaagttg acgttaatca a
//
LOCUS       DAZPAC010000653          437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZPAC010000653 DAZPAC010000000
VERSION     DAZPAC010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.671"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>437
                     /locus_tag="ACHUPM_004977"
     CDS             <1..>437
                     /locus_tag="ACHUPM_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFR0872458.1"
                     /translation="IQETKNMLDQFENRYPDLLKEVFRHVTIQRISEVLQRLLGENIS
                     VRNLKLIMESLALWAPREKDVITLVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDA
                     IRKGIRQTSGGSFLNMDIEVSDEVMETLAHALRELRNAKKNFV"
BASE COUNT          146 a           57 c           96 g          138 t
ORIGIN      
        1 tatacaagaa acaaaaaata tgttagatca gtttgaaaat cggtatcctg atctattaaa
       61 ggaagtcttc cgacatgtga ctatacagag aatttctgag gtattacaaa gattgcttgg
      121 agaaaatatt tctgttcgca atttaaaact tattatggag tctttggcgc tttgggctcc
      181 cagagaaaaa gatgtcataa cattagttga acatgtccgt gcatcacttt ctaggtatat
      241 ttgtagtaaa atagctgttt ctggtgagat taaagttgtg atgctttccg gatatattga
      301 ggatgcaata agaaaaggga taaggcaaac ctctggtggc tctttcttga atatggatat
      361 agaggtttcg gatgaggtaa tggaaacttt agcacatgct ttgagagaat tgagaaatgc
      421 aaaaaaaaat ttcgttc
//
LOCUS       DAZPAC010000654          436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZPAC010000654 DAZPAC010000000
VERSION     DAZPAC010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.672"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>436
                     /locus_tag="ACHUPM_004978"
     CDS             <1..>436
                     /locus_tag="ACHUPM_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0872459.1"
                     /translation="TLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRH
                     WMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEV"
BASE COUNT          127 a          113 c          122 g           74 t
ORIGIN      
        1 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
       61 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      121 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      181 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      241 atggataaag gttattactc actgggactg ttaaatgcct ggagcctggc gggagaacac
      301 cgccactgga tgatacctct cagaaaggga gcgcaatatg aagagctcag aaaactgggt
      361 aaaggcgatc atctggtgaa gctgaaaacc agcccgcagg cacgaaaaaa gtggccggga
      421 ctgggaaatg aagtga
//
LOCUS       DAZPAC010000655          427 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZPAC010000655 DAZPAC010000000
VERSION     DAZPAC010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.673"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>427)
                     /locus_tag="ACHUPM_004979"
     CDS             complement(<1..>427)
                     /locus_tag="ACHUPM_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872460.1"
                     /translation="GTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTLLAAVGAN
                     NVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFFALQNGHA
                     DSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTK"
BASE COUNT          129 a           90 c           65 g          143 t
ORIGIN      
        1 cctttgtgtg cccattttga agtgccataa acaacccatg ctttactttc aataattctt
       61 taatttgttc actactaagc attttcttat taagcagact tccgaacata cgaatactat
      121 ctgcatgtcc attctgcaat gcaaaaaata acccaggtaa atcatggctg tctttcgcag
      181 ctaatagata taatctctta tcggaagtta acggggtttt aaagactatt tccccatagg
      241 cttttattgt gtctacatgt ccgttcataa aagacatgca taaacccggc acattgtttg
      301 cccctaccgc agcaagcaat gtaagtattg tttcttgact aagattgcac atatttaata
      361 tcttaccata tacttgaata atgtctgaac atccgttttg aagtgctata taaaatgcag
      421 gagtacc
//
LOCUS       DAZPAC010000656          420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZPAC010000656 DAZPAC010000000
VERSION     DAZPAC010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.674"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            41..116
                     /locus_tag="ACHUPM_004980"
     tRNA            41..116
                     /locus_tag="ACHUPM_004980"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAZPAC010000657          415 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZPAC010000657 DAZPAC010000000
VERSION     DAZPAC010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.675"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            10..168
                     /locus_tag="ACHUPM_004981"
     CDS             10..168
                     /locus_tag="ACHUPM_004981"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0872461.1"
                     /translation="MELQDWRKEPRKKYLVQIVGSFAEFERAMLRERTKSGLAAARQD
                     GLVDGDQS"
BASE COUNT          113 a          102 c          110 g           90 t
ORIGIN      
        1 cacctgagta tggaattaca ggactggcga aaagaacctc gtaaaaagta tttggtgcaa
       61 atcgttggtt cctttgctga atttgaacgc gccatgcttc gtgaacggac aaagagtgga
      121 cttgcagcgg cacgccagga cgggttggtg gatggcgacc aaagttaaca ccccagcaac
      181 aaaaagagat tgtctcgctc gtcacatcgg ggcagaaaac aggtgctgat gccgcccgtc
      241 tgtttcgggt tcacccttcc accgtggtgc gtcttctcgc cagacaccgg atgactgaga
      301 tcaaacagac ataatctatg cggagattta cagcagggca tggttcacaa atctgacagc
      361 gatgaattat cggcactgcg ggcagaaaat gctcgaatca tcaagcccct gttac
//
LOCUS       DAZPAC010000658          410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZPAC010000658 DAZPAC010000000
VERSION     DAZPAC010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.677"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(279..>410)
                     /locus_tag="ACHUPM_004982"
     CDS             complement(279..>410)
                     /locus_tag="ACHUPM_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HFR0872462.1"
                     /translation="VNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          110 a           93 c           81 g          126 t
ORIGIN      
        1 cctcactccc gtaaagacgg gagtcaagtt cccgctccga cataactgcg tgcaatggat
       61 tttgaaggaa ccgtcagccc attccccgga ggcaaacatg ggctggcaaa agtgtagcgg
      121 tattaggcac agctatttag gaaagaggga ccatctccat ctcattaaat ggaccacgcc
      181 agacaattca ctgtcatgct gatgctgcac tgtggggaca gaatcactca tgatgacttc
      241 gataatcgac gacattatta tttggccgag cttttaggtc atatctcaaa tcgtttgcct
      301 aatattgctc cattttttaa taaaaaatca atcatttttg aatctttgct tttcattgca
      361 ttgtcaagca tagtgtcccc actatttgct ttttgaaatt ttttgtttac
//
LOCUS       DAZPAC010000659          408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZPAC010000659 DAZPAC010000000
VERSION     DAZPAC010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.678"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            54..130
                     /locus_tag="ACHUPM_004983"
     tRNA            54..130
                     /locus_tag="ACHUPM_004983"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="ACHUPM_004984"
     tRNA            173..248
                     /locus_tag="ACHUPM_004984"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAZPAC010000660          407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZPAC010000660 DAZPAC010000000
VERSION     DAZPAC010000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.679"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            30..206
                     /locus_tag="ACHUPM_004985"
     CDS             30..206
                     /locus_tag="ACHUPM_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872463.1"
                     /translation="MSFIFNEETLYFTVFGGDFIMDDLKIVKNTEAWVWYKNCDSKNK
                     KVAVELLQIYELHQ"
BASE COUNT          149 a           45 c           94 g          119 t
ORIGIN      
        1 aaggggtacg aataaagtaa cggggggcag tgagttttat ttttaatgag gaaacattgt
       61 attttactgt ttttggtggg gattttatta tggatgattt aaaaatagta aaaaatacgg
      121 aagcatgggt atggtataaa aattgcgata gtaaaaataa aaaagtggca gttgaacttc
      181 ttcaaatcta cgaattgcat caatgaaaaa tatagttaaa tagaggaggt tattaatagc
      241 aggaaaagca tcacgtgatg ctgatttttt gcactaaata attcgcgcta atgttcaata
      301 gttaatatat ccctcatcag ggaaagatca cggtgtgccg taggcacaaa gatgcaaaac
      361 gggacgtttt gcatgtaaca tgtaataagc ataagcttgt gaagaga
//
LOCUS       DAZPAC010000661          400 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZPAC010000661 DAZPAC010000000
VERSION     DAZPAC010000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.680"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(105..>400)
                     /locus_tag="ACHUPM_004986"
     CDS             complement(105..>400)
                     /locus_tag="ACHUPM_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HFR0872464.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT          122 a          107 c           93 g           78 t
ORIGIN      
        1 caatatgtgg cttagctcat gaaaggagcg caacaagatg atgaatcaac atataacaac
       61 atcttaaaaa aaggcctgac attacgccag gccttctgcg ttaattaatc acgcttttcc
      121 agcagggtcc ggtaaatcag accaccgata atgccgccga caattggcac cacccagaag
      181 aaccacagtt gttctaatgc ccagccgccc tggaagatag caaccgcggt gctgcgcgcc
      241 gggttaacag aagtgttagt caccggaata ctaattaagt gaatcagggt taaggccaga
      301 ccaatagcga tcggcgcaaa acctgccggc gcgaatttgt cggttgcgcc gtggatcacc
      361 aacaggaaac ctgcactcaa taccagttca actaccagcg
//
LOCUS       DAZPAC010000662          399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZPAC010000662 DAZPAC010000000
VERSION     DAZPAC010000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.681"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>399
                     /locus_tag="ACHUPM_004987"
     CDS             <1..>399
                     /locus_tag="ACHUPM_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR0872465.1"
                     /translation="QSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSL
                     NDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAGR
                     INSRSTGSVEGGKSRLSVADAITRVMIVGMT"
BASE COUNT          105 a           65 c          110 g          119 t
ORIGIN      
        1 tcaatctaaa agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt
       61 cgattcacta accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc
      121 ttatcgttca ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt
      181 aagtgcatta aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg
      241 tggtatctgg aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc
      301 aggaagaatc aattcacgtt ctactggtag tgttgaaggt ggaaaaagta ggctatctgt
      361 agctgatgca attaccagag taatgattgt cggtatgac
//
LOCUS       DAZPAC010000663          398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZPAC010000663 DAZPAC010000000
VERSION     DAZPAC010000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.682"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>398)
                     /locus_tag="ACHUPM_004988"
     CDS             complement(<1..>398)
                     /locus_tag="ACHUPM_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR0872466.1"
                     /translation="VWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFI
                     GRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSAD
                     LFLETTDLPCWRHGEWLHLCSIALSAEPSR"
BASE COUNT          105 a          120 c           89 g           84 t
ORIGIN      
        1 acgcgaaggc tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca
       61 gcagggcaag tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa
      121 tgaacgttcc ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc
      181 ttgcttcaga tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc
      241 aaaaggatca tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc
      301 caccgcaacg ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg
      361 caagagatct acgaccgcat cccctaaaac ccatactt
//
LOCUS       DAZPAC010000664          397 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZPAC010000664 DAZPAC010000000
VERSION     DAZPAC010000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.683"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>397
                     /gene="icsB"
                     /locus_tag="ACHUPM_004989"
     CDS             <1..>397
                     /gene="icsB"
                     /locus_tag="ACHUPM_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR0872467.1"
                     /translation="AESAFAAYEAVKNSIQHSFTGKDIKLMLNTAERFHGIGTAKNLE
                     RHLVFRCWGNRGITHLGHTSISIKNNLLQEPTHTYLSWYPGGNVTKDTEINYLFEKRS
                     GYSVDTYKQDKLNMISDQTAERLDAGQEV"
BASE COUNT          144 a           79 c           75 g           99 t
ORIGIN      
        1 ctgcagaaag tgcgtttgcc gcctatgaag cagtcaaaaa ttcaattcaa cattctttta
       61 caggtaaaga catcaaatta atgttgaata ccgcagagcg ttttcatggc atcggtacag
      121 ccaaaaatct tgaaagacat ttagtttttc gttgctgggg aaatagaggc ataacccatt
      181 tagggcatac cagtatcagt ataaaaaaca atttactaca ggaaccaact catacctatc
      241 tcagctggta tcccggcggg aatgttacaa aagatacaga aataaactac ctttttgaaa
      301 aacgctcagg ttacagtgtc gatacctata aacaagacaa actaaatatg atttcagacc
      361 aaacagccga aagacttgat gcaggccaag aggttag
//
LOCUS       DAZPAC010000665          396 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZPAC010000665 DAZPAC010000000
VERSION     DAZPAC010000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.684"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..250)
                     /locus_tag="ACHUPM_004990"
     CDS             complement(<1..250)
                     /locus_tag="ACHUPM_004990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872468.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEI"
BASE COUNT          110 a           92 c           85 g          109 t
ORIGIN      
        1 caatttccgg atacccttcg tcatcaacac gtacaaacca gaagaccagt tttttgtttc
       61 cggcatccac aaagaacgga atattcaggt ctgcgcaaca ctcaacggca tcgtcaaaat
      121 tatcaaagcg cagaacctca gcttcttctt cgtcaaaaaa atcatcttcg tgaagtttta
      181 cgacatagcg gaggagtttg cttctttgag gtgcaggttt tcgtttgcgt ggtttagctg
      241 aacgggccat ataactacct gaaagacaat gatattgtct gtttttataa cggtaatcac
      301 aggccatgac aagccgcagc cgtcaggccg cctactcggg gcggtttaga acgcttattt
      361 agtaccagat cttacttata ataaaggggc ttggca
//
LOCUS       DAZPAC010000666          395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZPAC010000666 DAZPAC010000000
VERSION     DAZPAC010000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.685"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
BASE COUNT          126 a          104 c           67 g           98 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcata
//
LOCUS       DAZPAC010000667          395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZPAC010000667 DAZPAC010000000
VERSION     DAZPAC010000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.686"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..356)
                     /locus_tag="ACHUPM_004991"
     CDS             complement(<1..356)
                     /locus_tag="ACHUPM_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR0872469.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTA"
BASE COUNT          103 a           99 c          117 g           76 t
ORIGIN      
        1 tgcgctgttt tcgtgaacac gcggcggaca gcctcacttc ccaggcgctg gcgggcctga
       61 ataacggcac tgggggcaac gaaggggcga ttgcccggca gcatgatgtc caggcgattc
      121 acaatctggt gaagaggttc tttacgctca agcgccatgc caacaataca ccagaccatc
      181 atttcgaggg gaagacggcg cttgcgtagc gttacagtac ctgattcggc aaggcaacga
      241 gagatgagtt cggggtcgag gtaatccccc agagaagtca gtgggttacg cagagaatcg
      301 taacgggata ccagatcaag agcctgtcca atgtgcataa aaaaatccgg aaacaagtga
      361 gcgtttccgg attcttacac agccactgga tcggt
//
LOCUS       DAZPAC010000668          393 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZPAC010000668 DAZPAC010000000
VERSION     DAZPAC010000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.687"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            63..>393
                     /gene="yhaV"
                     /locus_tag="ACHUPM_004992"
     CDS             63..>393
                     /gene="yhaV"
                     /locus_tag="ACHUPM_004992"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR0872470.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT          120 a           72 c          100 g          101 t
ORIGIN      
        1 ggaaagaaac ttgtcactgg catggacgtc aacattgatg atgagattgg cgacgacgaa
       61 taatggattt tccacaaagg gttaatggtt gggcgctata tgctcatccc tgttttcagg
      121 aaacctacga cgctttagtt gccgaagtcg agacattaaa gggaaaagat cctgaaaatt
      181 atcagagaaa agccgccaca aagttattgg cggtagtcca taaagtgatt gaggagcata
      241 tcacggtcaa tccatcatca ccggcattcc gtcatggcaa gtcgttaggc tctgggaaaa
      301 ataaagactg gtcacgggta aaatttggtg caggtcgtta tcgtctcttc tttcgttata
      361 gtgaaaaaga gaaagtcatc attctgggat gga
//
LOCUS       DAZPAC010000669          391 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.688, whole genome shotgun sequence.
ACCESSION   DAZPAC010000669 DAZPAC010000000
VERSION     DAZPAC010000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.688"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            40..276
                     /locus_tag="ACHUPM_004993"
     CDS             40..276
                     /locus_tag="ACHUPM_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFR0872471.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
BASE COUNT          147 a           55 c           54 g          135 t
ORIGIN      
        1 ctatcaaaag tattaaagaa tatcaataag gattaaaata tgataaaaga aaaaatatta
       61 tcaatagtgg ccttttgcta tggtatagcg tacagcaaac tatctgaaga aactaaattt
      121 attgaagatc tttctgccga ttctctttct ctgattgaaa tgctggatat gattagtttt
      181 gagttcaatc tacgtataga tgaatctgca ttagaacaca ttattactat tggagatctt
      241 atttctgtag tcaaaaatag tacaaaatca atatagtaat tgataagcat tttttcatct
      301 atggagctct cacatcagag ctccacaaga atattattct tttatccaat aaagataaat
      361 tgcatcaatc cagctattaa aatagtaatt t
//
LOCUS       DAZPAC010000670          389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.689, whole genome shotgun sequence.
ACCESSION   DAZPAC010000670 DAZPAC010000000
VERSION     DAZPAC010000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.689"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(215..277)
                     /locus_tag="ACHUPM_004994"
                     /pseudo
     CDS             complement(215..277)
                     /locus_tag="ACHUPM_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          124 a           95 c           84 g           86 t
ORIGIN      
        1 cccgcctgcg ggcactgttg cagacgcaac aggacactat ccaccagatg gctgaataca
       61 accgcctgct ctcacagcgg atggcggctt atgcttccga aatcaaccga ctgaaggcgc
      121 tggttgcgaa actgcagcgt atgtagttcg gtaaaagctc agaaaaactt cgcgcaaaaa
      181 ccgaacggca gattccattt tcacgggcga tctacgccac acaggctctg ggttgcagtg
      241 actgctccat taaggcgatt ttgaactcat aagggaagta acaaaaaaac tgcaaaagta
      301 caataattat atatggccaa actaacctct attaaaaaaa catcagtttt tcacacttag
      361 ttgagcttca tgaaaaaatc atcgaggat
//
LOCUS       DAZPAC010000671          389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.690, whole genome shotgun sequence.
ACCESSION   DAZPAC010000671 DAZPAC010000000
VERSION     DAZPAC010000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.690"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            130..388
                     /locus_tag="ACHUPM_004995"
                     /pseudo
     CDS             130..388
                     /locus_tag="ACHUPM_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          126 a           79 c           94 g           90 t
ORIGIN      
        1 cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc atcccctaaa
       61 cggtcaaaat gcttctgccg tgaaggaggg agaatgccat ctcatcactt atatctggcg
      121 aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa tggaatggca
      181 gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga acctgacgtg
      241 ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat ggcatcctca
      301 tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca gtaatgggga
      361 aaatgatagc tggtttctga aaatataga
//
LOCUS       DAZPAC010000672          389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.691, whole genome shotgun sequence.
ACCESSION   DAZPAC010000672 DAZPAC010000000
VERSION     DAZPAC010000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.691"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            251..>389
                     /locus_tag="ACHUPM_004996"
     CDS             251..>389
                     /locus_tag="ACHUPM_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HFR0872472.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RS"
BASE COUNT          121 a           69 c           65 g          134 t
ORIGIN      
        1 ctaaacttaa taaaacctta agattaacat tttaatataa cgagttgcat ttagttacat
       61 gtttaacact tgatttaaga tttgtaatgg ctagattgaa atcagatgta atccattagt
      121 tttatatttt acccatttag ggctgattta ttactacaca cagcagtgca acatctgtca
      181 gtacttctgg tgcttctatt ttagaggcag ctgtcaggtg tgcgatcaat aaaaaaagcg
      241 gggtttcatc atgtttaatg aagtccatag tattcatggt catacattat tgttgatcac
      301 taaaccttct ttgcaggcga cggctctctt gcagcacctt aaacaatcgc tggcaattac
      361 aggaaaatta cataatattc aacgttctc
//
LOCUS       DAZPAC010000673          387 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.693, whole genome shotgun sequence.
ACCESSION   DAZPAC010000673 DAZPAC010000000
VERSION     DAZPAC010000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.693"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            12..119
                     /locus_tag="ACHUPM_004997"
     CDS             12..119
                     /locus_tag="ACHUPM_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0872473.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           93 a           85 c          126 g           83 t
ORIGIN      
        1 caggcggcta tatgacgctc gcagaactgg gcatggcctt ctggcatgat ttagcggctc
       61 cggtcattgc tggtattctt gccagtatga tcgtgaactg gctgaacaag cggaagtaac
      121 gtgccatgcg ggcgtcaggc tgccgtaatg gcaatttgcg cccggatcag gccgcagggg
      181 tgaaactctg cggccttttt cgtaagaaaa atgcaacggt tgcaagcgga taatattctg
      241 cggaatgcgc agcacagagt gaagacgtga cggattccgg aacagaaaaa cagtcgtaga
      301 atcagcgtca agccggaagg gttccggtga ggcgcaatgt tgcgggggct ttatccctgg
      361 tggcattggt tgctggagag agaaaac
//
LOCUS       DAZPAC010000674          386 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.694, whole genome shotgun sequence.
ACCESSION   DAZPAC010000674 DAZPAC010000000
VERSION     DAZPAC010000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.694"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>386
                     /locus_tag="ACHUPM_004998"
     CDS             <1..>386
                     /locus_tag="ACHUPM_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872474.1"
                     /translation="LYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNR
                     FVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIV
                     IPLMIIPMLGSNTVQDLMNTIINMTA"
BASE COUNT           81 a           98 c          102 g          105 t
ORIGIN      
        1 actgtattcc gtctcctcgg tgctgattaa cgtcttcgtc agcagcaaag agttgaaatt
       61 taccggcgga tcggtgcagg tattccacgg catggcggcg tattttggtc taccggaagc
      121 gttgatgaat cgctttgtcg gtctggtgtc ctttaccgcg atgttcggtt ccctgctgat
      181 gtggaccgca acgccggtga aaattttctt ctccgaaatc ccggaaggca tctttggtaa
      241 gaaaaccgtc gaactgaacg aaaacggcgt tccggcgcgc gcagcgtgga tccagttcct
      301 gatcgtcatc ccgctgatga ttatcccgat gctcggttcc aataccgtgc aggatctgat
      361 gaatactatt attaatatga ccgccg
//
LOCUS       DAZPAC010000675          380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.695, whole genome shotgun sequence.
ACCESSION   DAZPAC010000675 DAZPAC010000000
VERSION     DAZPAC010000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.695"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>380
                     /locus_tag="ACHUPM_004999"
     CDS             <1..>380
                     /locus_tag="ACHUPM_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872475.1"
                     /translation="RVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKR
                     VVKKEHADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGKLTSVILPDGRTVRYG
                     YNSQRQVTSVTYPDGLRSSREYDE"
BASE COUNT           85 a           87 c          155 g           53 t
ORIGIN      
        1 gtcgggtgac ggagcaggtc aacccggagg ggctggacta ccgctttgag tacggagaga
       61 gccgggtaat catcacggac agcctgaacc ggcgggaggt gctgtacacg gaaggcgagg
      121 gtggcctgaa gcgggtggtg aagaaggagc atgccgacgg gagcatcacc cgcagcgagt
      181 atgatgaggc ggggaggctg aaggcgcaga cggatgcggc gggacggcgg acggagtacc
      241 gactgcatat ggcgtctgga aagctgactt cagtgatact gccggacggc aggacggtac
      301 ggtatggcta taacagccag cggcaggtga cgtcggtgac gtacccggac gggcttcgca
      361 gcagccggga gtatgatgag
//
LOCUS       DAZPAC010000676          379 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.696, whole genome shotgun sequence.
ACCESSION   DAZPAC010000676 DAZPAC010000000
VERSION     DAZPAC010000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.696"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
BASE COUNT          116 a           64 c           78 g          121 t
ORIGIN      
        1 agtcacataa accaccgatt gagtgacagt tactgaaaca actatttcga cacaaaatct
       61 cgtctattca acatcatgca aatttgtcag tgactgaatt tagtgacaga accctctgga
      121 attctgccgt gtaaccactt atatgcagac ctgccccgtt gctctgggtt ttcaggctgc
      181 gatgagtgat gagcgatgat ttccatacag atgatatatc gactggaatt gattgcctgc
      241 aattagattt agttttattg tgtggatcgt tttagttagt ggggatgaat ttccgataaa
      301 ttaaccaatg gaaaggagat gatatgaata aaaagataac tggattatta ataattagtc
      361 tggcgtcaat tttcttaat
//
LOCUS       DAZPAC010000677          374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.697, whole genome shotgun sequence.
ACCESSION   DAZPAC010000677 DAZPAC010000000
VERSION     DAZPAC010000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.697"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>374
                     /locus_tag="ACHUPM_005000"
     CDS             <1..>374
                     /locus_tag="ACHUPM_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0872476.1"
                     /translation="SRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQ
                     IRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPH
                     EYNGGLPPNESENRYWKNSNSVA"
BASE COUNT          106 a           87 c          111 g           70 t
ORIGIN      
        1 agcaggctca ccatgaaagc gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg
       61 gtgatgttcc acagcgatca gggcagtcat tatacgagca ggcagttccg gcagttattg
      121 tggcgatacc agatcagaca gagtatgagc cggcgcggaa actgctggga taacagccca
      181 atggaacgct tcttcaggag tctgaagaac gaatggatgc cggtggtggg ttacgtaagc
      241 ttcagcgagg cagctcacgc cataacggac tatatcgttg gatattacag cgcactaaga
      301 ccgcacgaat ataacggtgg gttaccccca aacgaatcgg aaaatcgata ctggaaaaac
      361 tctaactcgg tggc
//
LOCUS       DAZPAC010000678          369 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.698, whole genome shotgun sequence.
ACCESSION   DAZPAC010000678 DAZPAC010000000
VERSION     DAZPAC010000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.698"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
BASE COUNT          131 a           59 c           63 g          116 t
ORIGIN      
        1 attattacaa aaaatcttca ccaaattttt taaccgctgt ttgttgtaat atttttgcca
       61 tatttatagt aacgaattgg aataagtaac gacaacttag ggaataactc gatataaact
      121 cacctttgta agggggatgt taaatatata ttaattcctg tgttcctgat tttgcccggt
      181 aatagagaat tattctctga gaagctcatc atgattgcac catacaaata tcatgatgat
      241 aagaggatat tattcattga tttaaacaaa atgacaaaat ccatagaccc atagtctggg
      301 tactatatag gagtggtatg ggcatcaaac aacacaatgg gaataccaaa gcagatcgtc
      361 tcgctgaat
//
LOCUS       DAZPAC010000679          367 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.699, whole genome shotgun sequence.
ACCESSION   DAZPAC010000679 DAZPAC010000000
VERSION     DAZPAC010000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.699"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..367
                     /locus_tag="ACHUPM_005001"
                     /pseudo
     CDS             1..367
                     /locus_tag="ACHUPM_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT          104 a           74 c           82 g          107 t
ORIGIN      
        1 actttcctga agacacttat gatagccgag cacgtgctcg ctctttagaa tatattgaac
       61 aaaactataa tgaaaaagag atctgtccct atgcgcttat ggtgtatggt gtatggtgtc
      121 ggtgatggcg gcgctggccc cggtgaggaa catatagaac gattaacacg tatacgcaat
      181 attgatggct tacctcatgt tgatttcagc cgtgttgata agttcttcac ttacgccgac
      241 gcctttcgcg aaagcttgcc cattatttca ggtgaacttt atttcgaggc tcatcaaggg
      301 tgctttacat cagagtcagc aactaaagct cataatcgaa atatggaaaa taaattacat
      361 gatgctg
//
LOCUS       DAZPAC010000680          364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.700, whole genome shotgun sequence.
ACCESSION   DAZPAC010000680 DAZPAC010000000
VERSION     DAZPAC010000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.700"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
BASE COUNT          122 a           45 c           90 g          107 t
ORIGIN      
        1 tagtagtata cttgtaaccg tgcattttgt gtattcttta ttttttaaaa gtggaaaggt
       61 aaaagataag gtaaaaaaat ggattaaagt aatgtgtgat ttattttggg ggataggata
      121 gtggtgtgat atggataaat ataaattaca gtggctggca tatttcgacc tgacgccgga
      181 gtagataaaa aaacagacag gattggagta tagcaagcgt ctgacaatct ctcgggacag
      241 tagtatgatg aagaggcggg gttgtactat aaccgtaacc ggtactacga tccgctaaag
      301 ggcaagtata tgactcaggg aaagatcatc agaagttcac tttttgtact aaataattcg
      361 catt
//
LOCUS       DAZPAC010000681          364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.701, whole genome shotgun sequence.
ACCESSION   DAZPAC010000681 DAZPAC010000000
VERSION     DAZPAC010000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.701"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..347
                     /locus_tag="ACHUPM_005002"
     CDS             <1..347
                     /locus_tag="ACHUPM_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR0872477.1"
                     /translation="VLTRGDVRHIAQDWSLTDDELETVMQRLDDAFEHGADVSVVHDV
                     VRELMEEKRASRQVTVPAVMLEKVLALAGSEMKRLYAVGSENGGDGDAFVREEREAMD
                     VVLQALDGEHMS"
BASE COUNT           85 a           78 c          132 g           69 t
ORIGIN      
        1 tggtgctgac ccgtggggat gtgcgtcaca tagcccagga ctggagtctg acagacgatg
       61 agctggaaac cgtcatgcag cggctggacg atgcctttga gcatggtgcg gatgtcagcg
      121 ttgttcacga cgttgttcgt gaactgatgg aagaaaagcg cgccagccgt caggtgacag
      181 tcccggcggt gatgctggaa aaagtgttgg cgctggcagg cagtgaaatg aagcgcctgt
      241 atgccgtcgg gagtgagaac gggggcgacg gtgatgcgtt cgtcagggaa gaacgcgaag
      301 caatggacgt tgtgttacag gcgctggacg gggagcacat gtcatgaata tcagcacaga
      361 aacc
//
LOCUS       DAZPAC010000682          358 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.702, whole genome shotgun sequence.
ACCESSION   DAZPAC010000682 DAZPAC010000000
VERSION     DAZPAC010000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.702"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>358
                     /locus_tag="ACHUPM_005003"
     CDS             <1..>358
                     /locus_tag="ACHUPM_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFR0872478.1"
                     /translation="WLNWLEKQPERAVRPVVIESVTKIMACGTTLMGYTQWCCSSPDC
                     CHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVFTLPCQYWSLVFHNR
                     WLLTEMSRIAADVILEI"
BASE COUNT           77 a           92 c          110 g           79 t
ORIGIN      
        1 ctggcttaac tggctggaga agcagccgga aagggcagta cgtccggtgg tgattgagtc
       61 ggtgaccaaa atcatggcgt gcgggaccac actgatgggg tatacacagt ggtgctgttc
      121 atcaccggac tgttgccata caaaaaaggt ctgcttccgg tgtaaaagcc gctcctgccc
      181 gcactgcggg gtgaaggctg gcgcacagtg gatacagtat ctgctgagcc tggtccccga
      241 ctgcccgtgg cagcatattg tgttcacact tccctgccag tactggtccc tggtgttcca
      301 caaccggtgg ttactgacag agatgagccg cattgcagcg gatgtgatac tggaaatc
//
LOCUS       DAZPAC010000683          351 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.704, whole genome shotgun sequence.
ACCESSION   DAZPAC010000683 DAZPAC010000000
VERSION     DAZPAC010000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.704"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>351
                     /gene="ipgB1"
                     /locus_tag="ACHUPM_005004"
     CDS             <1..>351
                     /gene="ipgB1"
                     /locus_tag="ACHUPM_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HFR0872479.1"
                     /translation="RDNVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHN
                     GVVLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSN
                     MDLFNTQMKDLTNLV"
BASE COUNT          140 a           62 c           61 g           88 t
ORIGIN      
        1 cagggacaac gtcatagaca aaataaaaga tagtaattcg gggaatcaac tattttgttg
       61 gatgagccaa gagcgaacct cttatgtctc ttcaatgata aacagaagta tagatgaaat
      121 ggccatacac aatggagttg ttttgacctc agataacaaa aaaaacatat ttgcagctat
      181 tgaaaaaaaa ttcccggata taaaacttga tgaaaaatca gcacaaacat ctatcagtca
      241 tacagcacta aacgagattg cctcatctgg cctcagagca aagattttaa aacgctatag
      301 cagtaacatg gatttgttta acacacaaat gaaggatctt acaaatctag t
//
LOCUS       DAZPAC010000684          348 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.705, whole genome shotgun sequence.
ACCESSION   DAZPAC010000684 DAZPAC010000000
VERSION     DAZPAC010000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.705"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..270
                     /gene="istB"
                     /locus_tag="ACHUPM_005005"
     CDS             <1..270
                     /gene="istB"
                     /locus_tag="ACHUPM_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR0872480.1"
                     /translation="VLILDDLGLEQLSNAQCNDLLEITEDRYGQSSTIVVSQFPVDKW
                     HGLMENPTTADAILDRLVHNSHRVVLQGESLRKNPPTVESSEKTS"
BASE COUNT          110 a           76 c           94 g           68 t
ORIGIN      
        1 gttctaatcc tggacgatct ggggctggaa cagttaagta acgcccagtg taatgacctt
       61 ctggagatca cagaggatcg ttacggacaa agcagcacga tagtggtaag ccagttcccg
      121 gtagataaat ggcatggtct gatggagaac ccaacaacag cagatgccat cctggacaga
      181 ctggtacata actcacacag agtggtcctt cagggagagt ccctgaggaa aaatcccccg
      241 acagtggaaa gcagcgaaaa aacgagttaa aaagcggtag aaaaagttaa gttcccggtt
      301 gatcgaatgg atgcgaatcg ctgatcgaac ttcgatgcca atccgtga
//
LOCUS       DAZPAC010000685          340 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.706, whole genome shotgun sequence.
ACCESSION   DAZPAC010000685 DAZPAC010000000
VERSION     DAZPAC010000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.706"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>340
                     /locus_tag="ACHUPM_005006"
     CDS             <1..>340
                     /locus_tag="ACHUPM_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872481.1"
                     /translation="LQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLT
                     GVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGR
                     LTEKTDLIPEG"
BASE COUNT           82 a          124 c           88 g           46 t
ORIGIN      
        1 gttacagagc cagcacctga acagcctgct gtctgaccgc gattacacct ggaacgacaa
       61 cggcgaactc atccgcatca gcagcccgcg ccagacccgg agttacagct acagcaccac
      121 cggcaggctg accggcgttc acaccaccgc agcgaatctg gatatccgca tcccgtatgc
      181 cacagacccg gcggggaacc gcctgcccga cccggagctg cacccggaca gcaccctcag
      241 catgtggccg gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccatca
      301 cggcagactg acagagaaaa ccgacctcat cccggaaggg
//
LOCUS       DAZPAC010000686          338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.707, whole genome shotgun sequence.
ACCESSION   DAZPAC010000686 DAZPAC010000000
VERSION     DAZPAC010000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.707"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(39..>338)
                     /locus_tag="ACHUPM_005007"
     CDS             complement(39..>338)
                     /locus_tag="ACHUPM_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0872482.1"
                     /translation="SKSGVEETSVLWCFFNLFGVLIPIDERNLTRERTQVGLQAARAR
                     GRKGGRPKTLSKDKQALAVQLYNEKKHTVAQICVLMGISRPTLYKYIESARLFKK"
BASE COUNT           86 a           89 c           63 g          100 t
ORIGIN      
        1 ggatttgaag cccaaccgta cgaaaacgta cgctaagttc atttcttgaa caacctggct
       61 gactctatgt atttgtacag cgttggcctc gatatcccca tcaacacaca aatctgcgca
      121 actgtatgtt ttttctcgtt atagagttga acagcaaggg cctgtttatc cttactcagt
      181 gttttcggcc tgccgccctt acgtcctctg gctcgtgctg cttgaagccc gacctgagtt
      241 ctctctcttg tcaggttgcg ttcatcgata ggaattaaaa ccccaaaaag attaaaaaaa
      301 caccacaaaa cggatgtttc ttcaacacca cttttgct
//
LOCUS       DAZPAC010000687          334 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.708, whole genome shotgun sequence.
ACCESSION   DAZPAC010000687 DAZPAC010000000
VERSION     DAZPAC010000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.708"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..301)
                     /locus_tag="ACHUPM_005008"
     CDS             complement(<1..301)
                     /locus_tag="ACHUPM_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0872483.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRED"
BASE COUNT          102 a           71 c           72 g           89 t
ORIGIN      
        1 aatcctctct cggcacggtt gcaattagcg agtgggtagt atcaccaaca gcgcgaaatc
       61 cgcgtctgat aatggatatg ttctgatata acgtgttagt aggcacacgc ataccttctt
      121 cttcccagac tctggtgaaa aaatcttgtt gtgcaaccac atcaggaaaa gcttcaagta
      181 gaagtgataa acatcgactg gctggcgttg tcagaacgac gttaagatcg gcgttatgta
      241 ccgatattaa ttttcggtgc tcaggccaga actcgatatt atcgttaata atccagtaca
      301 tacgtcaata aacactaaat caatcgaaat ggag
//
LOCUS       DAZPAC010000688          330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.709, whole genome shotgun sequence.
ACCESSION   DAZPAC010000688 DAZPAC010000000
VERSION     DAZPAC010000688.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.709"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(3..>330)
                     /locus_tag="ACHUPM_005009"
     CDS             complement(3..>330)
                     /locus_tag="ACHUPM_005009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872484.1"
                     /translation="SQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCADLNI
                     PFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVHWPD
                     GVAPPF"
BASE COUNT           86 a           95 c           79 g           70 t
ORIGIN      
        1 catcagaagg gcggggcgac tccgtccggc cagtgaaccg tgccacactc cgggcagtac
       61 ataccgccgg cgctgatacc ggaaagaatg gtcgcaaatt cccgctccgt gcagcgggcg
      121 atttccggat acccttcgtc atcaacacgt acaaaccaga agaccagctt tttgtttccc
      181 gcatccacaa agaacggaat attcaggtct gcgcagcatt caacggcatc gtcaaaacta
      241 tcaaagcgca gaacttctgc gtcttcttcg tcaaaaaaat catcttcgtg aagcttcacg
      301 acatagcggg gaagtttgct tctttgagag
//
LOCUS       DAZPAC010000689          330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.710, whole genome shotgun sequence.
ACCESSION   DAZPAC010000689 DAZPAC010000000
VERSION     DAZPAC010000689.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.710"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..228)
                     /locus_tag="ACHUPM_005010"
     CDS             complement(<1..228)
                     /locus_tag="ACHUPM_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFR0872485.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAP"
     gene            complement(225..>330)
                     /locus_tag="ACHUPM_005011"
     CDS             complement(225..>330)
                     /locus_tag="ACHUPM_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0872486.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT           83 a           96 c           83 g           68 t
ORIGIN      
        1 cggggcaagt tccggcatct cattacacaa cagcacgggg tcaatcactc ctgaaacacg
       61 ggcctgtctg cagatgcctg tgtagatatc ccgatacgct cgtacagaaa aaatgtccgc
      121 cggtagtgtg atcagaatat ccatcacttc atgatctgcc ccacgcagaa agaatgcgcc
      181 aatgacagcg ccttccaggt catcattacg ccagactggt gttgtcatgc agccacacct
      241 ctgatacaag aacggtagct gggccagttg aacgacaacc agttgcgccc cccgtctgtg
      301 atcctgtcgg caatgcgggg gctgatgaac
//
LOCUS       DAZPAC010000690          329 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.711, whole genome shotgun sequence.
ACCESSION   DAZPAC010000690 DAZPAC010000000
VERSION     DAZPAC010000690.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.711"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            92..292
                     /gene="glgS"
                     /locus_tag="ACHUPM_005012"
     CDS             92..292
                     /gene="glgS"
                     /locus_tag="ACHUPM_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR0872487.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT           91 a           58 c           85 g           95 t
ORIGIN      
        1 tatgtttaaa ggcactacac tgattgggga atactgaaat cagaaaaagc atatggcctt
       61 aaaaggtatg cgataaccgg aggatgtgct tatggatcat agtcttaatt ctttaaataa
      121 tttcgatttc ctggcgcgta gttttgccag aatgcacgca gaaggtcgcc cggtcgatat
      181 tcttgccgtt actggtaaca tggatgaaga acatagaacc tggttttgcg cacgttatgc
      241 ctggtattgc caacagatga tgcaggcaag agagctggag ttagagcact gatataacgg
      301 gcctgatggc ccgttttagt gtttgtatt
//
LOCUS       DAZPAC010000691          328 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.712, whole genome shotgun sequence.
ACCESSION   DAZPAC010000691 DAZPAC010000000
VERSION     DAZPAC010000691.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.712"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..311)
                     /locus_tag="ACHUPM_005013"
     CDS             complement(<1..311)
                     /locus_tag="ACHUPM_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872488.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     V"
BASE COUNT          138 a           71 c           44 g           75 t
ORIGIN      
        1 attacaggga aaaacagtag ggtaatagca aaaaatagtc taaaaaaact caagtcttgt
       61 attttacttc ttttctttat aagaagaaaa aataaaatac caatccccca accggtaaaa
      121 agtataccca ttactatgat agcatagagt aaacttctgg caacatcatc caaacctgca
      181 ccaacaaccc accatggaaa tccatagtaa aaagaggttc cccagccata agaataagca
      241 ctgacccacc ctacacatcc catataggcg atgaataatg aagaatttct aagcaatgaa
      301 ttgtcattca taagaaacca actgataa
//
LOCUS       DAZPAC010000692          327 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.713, whole genome shotgun sequence.
ACCESSION   DAZPAC010000692 DAZPAC010000000
VERSION     DAZPAC010000692.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.713"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            64..>327
                     /locus_tag="ACHUPM_005014"
     CDS             64..>327
                     /locus_tag="ACHUPM_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0872489.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDT"
BASE COUNT           98 a           65 c           84 g           80 t
ORIGIN      
        1 tcaccatatt atagttttat ttgtgaatta aatcaattat ggcgatgaat tacaaggggt
       61 taaatgctgc cgcagcatag cgatattgaa atagcctggt atgcttcgat acagcaggag
      121 ccgaatggct ggaagaccgt caccacacag ttctacatcc aggaattcag tgagcatatt
      181 gcgccacttc aggatgctgt agatctggag atcgcaacgg aggaagaaaa ctcgttgctg
      241 gaagcctgga agaagtatcg ggtgttgctg aaccgtgttg atacatcaac tgcacctgat
      301 attgagtggc cggaagaacc agacaca
//
LOCUS       DAZPAC010000693          323 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.714, whole genome shotgun sequence.
ACCESSION   DAZPAC010000693 DAZPAC010000000
VERSION     DAZPAC010000693.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.714"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            162..>323
                     /locus_tag="ACHUPM_005015"
     CDS             162..>323
                     /locus_tag="ACHUPM_005015"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR0872490.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGI"
BASE COUNT          112 a           71 c           58 g           82 t
ORIGIN      
        1 cacgctgcaa tactccttag aatgtatagc gaaaggagaa aaagatatac ctcgatcacc
       61 cccctttctc ccaagtgaaa ataaaaggtt atcaacttgc aacattgaac aacattcgtt
      121 gcaaatcgat aacaacatgc accttcagga tactatttat tatgttcggc aatgatattt
      181 tcacccgcgt aaaacgttca gaaaataaaa aaatggcgga aatcgcccaa ttcctgcatg
      241 aaaatgattt gagcgttgac accacagtcg aagtatttat taccgtaacc cgcgatgaaa
      301 agcttatcgc gtgcggtgga att
//
LOCUS       DAZPAC010000694          321 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.715, whole genome shotgun sequence.
ACCESSION   DAZPAC010000694 DAZPAC010000000
VERSION     DAZPAC010000694.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.715"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
BASE COUNT           98 a           59 c           48 g          116 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacatttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga g
//
LOCUS       DAZPAC010000695          320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.716, whole genome shotgun sequence.
ACCESSION   DAZPAC010000695 DAZPAC010000000
VERSION     DAZPAC010000695.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.716"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(33..221)
                     /locus_tag="ACHUPM_005016"
     CDS             complement(33..221)
                     /locus_tag="ACHUPM_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872491.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
BASE COUNT           81 a           77 c           96 g           66 t
ORIGIN      
        1 cggggagtaa aagcggacaa catgaggacg gattaacccc cggtatcctg tagccgggta
       61 agggctgctg tgtcgccacg gcgctccagg atggaggccg cgaccatcgc aggtggtggg
      121 tccatgggtt tgagcatctt catccagtcc cattcacgtt gtgagagctt ctcagcacca
      181 acagagagca gaacggtatc tatacttttg cgggtcaaca taagaggatg agtccttaat
      241 aaaacaggtc ctcagagcat acatattctg cggagcgtgc ggcaacaaca ggacgaacag
      301 gggcggtttt caatttgcag
//
LOCUS       DAZPAC010000696          319 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.717, whole genome shotgun sequence.
ACCESSION   DAZPAC010000696 DAZPAC010000000
VERSION     DAZPAC010000696.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.717"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>319
                     /gene="virA"
                     /locus_tag="ACHUPM_005017"
     CDS             <1..>319
                     /gene="virA"
                     /locus_tag="ACHUPM_005017"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR0872492.1"
                     /translation="CSVFYLDGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAP
                     VSGNFKTHKPAPEVIETAINCCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASNSNST
                     KIYF"
BASE COUNT          117 a           62 c           44 g           96 t
ORIGIN      
        1 atgttcggtt ttttatcttg atggagataa aatacaatcc actcaacttt catcaaaaga
       61 atataataac ttgttaagtt cactcccccc caaacaattc aacttaggaa aagtacatac
      121 tattacagct ccggtatcag ggaattttaa aactcacaag cctgcaccag aagttattga
      181 aacagccatt aattgctgta catcgataat accaaacgat gattacttcc ccgttaaaga
      241 cactgatttt aatagtgttt ggcatgatat ttacagagat attagagcat ctaacagcaa
      301 tagtacaaaa atatatttc
//
LOCUS       DAZPAC010000697          312 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.718, whole genome shotgun sequence.
ACCESSION   DAZPAC010000697 DAZPAC010000000
VERSION     DAZPAC010000697.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.718"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(1..76)
                     /locus_tag="ACHUPM_005018"
     tRNA            complement(1..76)
                     /locus_tag="ACHUPM_005018"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Thr,seq:tgt)
     gene            complement(79..155)
                     /locus_tag="ACHUPM_005019"
     tRNA            complement(79..155)
                     /locus_tag="ACHUPM_005019"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Arg,seq:tct)
     gene            complement(157..233)
                     /locus_tag="ACHUPM_005020"
     tRNA            complement(157..233)
                     /locus_tag="ACHUPM_005020"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Ile,seq:cat)
BASE COUNT           71 a           87 c           81 g           73 t
ORIGIN      
        1 tggtgccgac taccggaatc gaactggtga cctactgatt acaagtcagt tgctctgcct
       61 gctgagctaa gtcggcgctg gcccaccacc gaggactcga acctcgcacc gtcaacttag
      121 aaggttgatg ctctatccgg atgagctagt ggtggttggt ggcccttgct ggacttgaac
      181 cagcgacctg gcgattatga gtcgctcgct ctgaccaact gagctaaagg gccggaggca
      241 gaataataac catatgtcat cacatctgca aactcatctg accaccagca cgtttaacgt
      301 cctgtgccgt tt
//
LOCUS       DAZPAC010000698          307 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.719, whole genome shotgun sequence.
ACCESSION   DAZPAC010000698 DAZPAC010000000
VERSION     DAZPAC010000698.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.719"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            77..>307
                     /locus_tag="ACHUPM_005021"
     CDS             77..>307
                     /locus_tag="ACHUPM_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR0872493.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPL"
BASE COUNT           99 a           68 c           71 g           69 t
ORIGIN      
        1 gctgattatt aaaaatcaac cacacccgcc agtattctgt atatttactg gcggtcatat
       61 cgtgagaggt atggcaatga atcttgtgac actcaaaacg tggggaaaac tcagatatcc
      121 ggataaccca ccatcaatat caacgctgag acgatgggca aggaatggaa acatttatcc
      181 tgcacctgaa ctacacggga ggagttacag ggtggttccg gaggctttct atatcaaccc
      241 aaataaggtt gataccgata taacacacca tcagcctaat gggcgacaag ggagagacag
      301 tccgtta
//
LOCUS       DAZPAC010000699          304 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.720, whole genome shotgun sequence.
ACCESSION   DAZPAC010000699 DAZPAC010000000
VERSION     DAZPAC010000699.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.720"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>304)
                     /locus_tag="ACHUPM_005022"
     CDS             complement(<1..>304)
                     /locus_tag="ACHUPM_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NEL-type E3 ubiquitin ligase domain-containing
                     protein"
                     /protein_id="HFR0872494.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKR"
BASE COUNT           69 a           84 c           79 g           72 t
ORIGIN      
        1 tacgcttcag tacagcatgc catggtcccc agagggagaa ccagtccgta aattcattct
       61 cttcacggct tctgaccatg gcttcggcag tgcggaggtc atttgctgtc actcccgaca
      121 cgccatagaa acgcatttcc ttcacggcag tggagagctg aagtttctct gcgagcatgg
      181 tctggaaggc caggtagact tctatctcat ccacaaaatg gagagttctg actttatccc
      241 gggcaatgtc ctccagaatt tcgaggcgga acatttccct gcccagggag agcagagcgc
      301 cggt
//
LOCUS       DAZPAC010000700          301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.721, whole genome shotgun sequence.
ACCESSION   DAZPAC010000700 DAZPAC010000000
VERSION     DAZPAC010000700.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.721"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>301
                     /locus_tag="ACHUPM_005023"
     CDS             <1..>301
                     /locus_tag="ACHUPM_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0872495.1"
                     /translation="PVWAKCTKGVYPSYWEEWKKVREVVKVACPECGGKGEVSTACKD
                     CRGRGVAIHREESVKRGMPVIRDCQRCGGRGYERLPSTEAFNAICEVTNQITRAS"
BASE COUNT           76 a           56 c          100 g           69 t
ORIGIN      
        1 cgccggtatg ggcaaagtgt acgaaaggtg tgtatccgtc ttactgggaa gaatggaaaa
       61 aagtcaggga ggtggtaaaa gttgcctgtc cggagtgtgg cggaaagggt gaggtttcca
      121 ccgcctgtaa ggattgccgt gggcgtggtg tcgccattca tcgtgaagag tcggtaaaac
      181 gtggtatgcc tgttatcaga gactgccagc gttgtggtgg tcgtggctat gaaagactac
      241 catcaacgga ggcatttaat gctatatgcg aggtgacaaa ccagataaca cgcgcgtcat
      301 g
//
LOCUS       DAZPAC010000701          301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.722, whole genome shotgun sequence.
ACCESSION   DAZPAC010000701 DAZPAC010000000
VERSION     DAZPAC010000701.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.722"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>301
                     /locus_tag="ACHUPM_005024"
     CDS             <1..>301
                     /locus_tag="ACHUPM_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872496.1"
                     /translation="DISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHET
                     RHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRS"
BASE COUNT           67 a           91 c          101 g           42 t
ORIGIN      
        1 gacatcagcc atctcagcga agggcaccgg gtgacggtgc attacgggta tgatgagaaa
       61 ggccggctga ccggtgagcg tcagacggtg catcacccgc agacggaagc actgctctgg
      121 cagcatgaga ccagacatgc gtacaacgcg caggggctgg cgaaccgctg tataccggac
      181 agcctgtccg ccgtggaatg gctgacctac ggcagcggct ggctgtcagg catgaaactc
      241 ggcgacacac cgctggtgga gtacacccgc gaccgcctgc accgggaaac gctgcgcagc
      301 t
//
LOCUS       DAZPAC010000702          293 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.724, whole genome shotgun sequence.
ACCESSION   DAZPAC010000702 DAZPAC010000000
VERSION     DAZPAC010000702.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.724"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>293)
                     /locus_tag="ACHUPM_005025"
     CDS             complement(<1..>293)
                     /locus_tag="ACHUPM_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraD N-terminal domain-containing protein"
                     /protein_id="HFR0872497.1"
                     /translation="RIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYW
                     WCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLA"
BASE COUNT           96 a           76 c           55 g           66 t
ORIGIN      
        1 gccagaacga atgcagacca taactgctca ccgcaccaga tcatatattt atcatgcagt
       61 acctgagcag cgttcatccg gaaggttttg ccgtaatact gaatctcata taccggctga
      121 gacttgatta aatcccgcat gccttccagc gtggtacacc accagtaaat acagccattc
      181 acaaacgtct gccagcttat ttttacccat aaaaccagac caacgagtat ccagaaaaaa
      241 ataaacaggc agtaaagcat aatattggcg atctggctga acatgcggat acg
//
LOCUS       DAZPAC010000703          292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.725, whole genome shotgun sequence.
ACCESSION   DAZPAC010000703 DAZPAC010000000
VERSION     DAZPAC010000703.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.725"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>292
                     /locus_tag="ACHUPM_005026"
     CDS             <1..>292
                     /locus_tag="ACHUPM_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR0872498.1"
                     /translation="RGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNG
                     GDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEW"
BASE COUNT           78 a           56 c           80 g           78 t
ORIGIN      
        1 atcgtgggcc gcaaagttta cgttgtctgc agttactgga acaacattgg gtttgtgcgg
       61 taagaggcaa tcatgaacag atggcgatgg atgcgctggc atcccagcag atgtctttgt
      121 ggttgatgaa tggcggcgac tggtttattg cgctggcaga taatcaacag aaacaagcga
      181 aaacggcgct ggaaaaatgt cagcatttgc cctttattct tgaagtacac agtcgtaccg
      241 gcaaacatgt tattgctcat gccgattatc cagatgatgt ttatgaatgg ca
//
LOCUS       DAZPAC010000704          292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.726, whole genome shotgun sequence.
ACCESSION   DAZPAC010000704 DAZPAC010000000
VERSION     DAZPAC010000704.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.726"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>292
                     /locus_tag="ACHUPM_005027"
     CDS             <1..>292
                     /locus_tag="ACHUPM_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0872499.1"
                     /translation="RLMGILSLKAAIKVKRYRSYRGEVGQTAPNVLQRDFKATRPNEK
                     WVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKL"
BASE COUNT           86 a           66 c           70 g           70 t
ORIGIN      
        1 cgcctgatgg gaatcctctc acttaaggca gcgattaagg tcaagcgata ccgctcttac
       61 agaggagagg tagggcaaac cgcccccaat gttctccaaa gagatttcaa ggctacgcgg
      121 ccaaacgaga agtgggttac cgatgttact gaatttgcag tcaatgggcg caagctgtat
      181 ttgtctccag taatagatct cttcaacaac gaagttattt cttacagcct ttcggaaaga
      241 ccagtgatga acatggtcga gaatatgctc gatcaggcat tcaaaaagct ta
//
LOCUS       DAZPAC010000705          291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.727, whole genome shotgun sequence.
ACCESSION   DAZPAC010000705 DAZPAC010000000
VERSION     DAZPAC010000705.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.727"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>291)
                     /locus_tag="ACHUPM_005028"
     CDS             complement(<1..>291)
                     /locus_tag="ACHUPM_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0872500.1"
                     /translation="LESALKEAVTEWRLYPVVEALQAMRGIQFITAVGLISELGDLTR
                     FEHPRQLMSWFGITPSEYSSGGSRHQGSITKAGNSYARKLLVEAAWSYRHPAR"
BASE COUNT           67 a           84 c           67 g           73 t
ORIGIN      
        1 gcgtgccgga tgacggtaac tccacgctgc ctcgaccaat agctttctgg catagctgtt
       61 tccggctttg gtaatgctgc cctgatgtct gcttccaccc gatgaatatt ctgatggcgt
      121 gataccaaac catgacatca gttgtcgtgg atgttcaaaa cgggtcagat cacccagttc
      181 agaaattaat ccaaccgcag taataaactg aatccctcgc atggcttgca gcgcctctac
      241 taccggatac agcctccatt cggtaacggc ctccttcagg gcggattcca g
//
LOCUS       DAZPAC010000706          289 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.728, whole genome shotgun sequence.
ACCESSION   DAZPAC010000706 DAZPAC010000000
VERSION     DAZPAC010000706.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.728"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..287
                     /locus_tag="ACHUPM_005029"
     CDS             <1..287
                     /locus_tag="ACHUPM_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HFR0872501.1"
                     /translation="DVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQYQLETKK
                     YVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT           92 a           58 c           73 g           66 t
ORIGIN      
        1 ttgatgttga tgcggcaatg aagaaagtct ctgaactgga aacgctggta gcgcaggcca
       61 aagaagcgga taaaggcggt atgaatttct cgttcattaa ttcggcagac cagtatcaac
      121 tcgagactaa aaaatatgtt cgccgcgtca gagataaagt cccgtactct gactgggata
      181 aagagcatct tcaggatgca aacacaagct ggatggtcga agactcattt ccacgagcgt
      241 tacgcgagta caacgaaatg gttgatgact ataatagcct gcgttaaac
//
LOCUS       DAZPAC010000707          287 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.729, whole genome shotgun sequence.
ACCESSION   DAZPAC010000707 DAZPAC010000000
VERSION     DAZPAC010000707.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.729"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>287
                     /locus_tag="ACHUPM_005030"
     CDS             <1..>287
                     /locus_tag="ACHUPM_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0872502.1"
                     /translation="GIFTVIHTWGRDQQWHPHIHLSTTAGGVTSGHTWKNLHFYARKV
                     MSMWRYRITRLLSRKYPDLVMPDALAAEGSSKREWNRFLDTHYRRGWNVNV"
BASE COUNT           69 a           72 c           86 g           60 t
ORIGIN      
        1 cggggatatt cacggtgatc cacacatggg ggcgtgacca gcagtggcat ccgcatatcc
       61 atttatcgac aactgccggt ggtgtgacgt cgggccacac ctggaaaaat cttcattttt
      121 acgcccgtaa ggtgatgagc atgtggcgct accggataac acggttgctg tcacggaaat
      181 accctgacct ggtgatgccg gatgcgctgg cagcagaagg aagcagtaaa cgggaatgga
      241 atcgcttcct ggacacgcat taccgccgtg gctggaatgt caacgta
//
LOCUS       DAZPAC010000708          286 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.730, whole genome shotgun sequence.
ACCESSION   DAZPAC010000708 DAZPAC010000000
VERSION     DAZPAC010000708.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.730"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>286)
                     /locus_tag="ACHUPM_005031"
     CDS             complement(<1..>286)
                     /locus_tag="ACHUPM_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR0872503.1"
                     /translation="SACTLNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTC
                     DSHFELSQLSAKDFFDNFYWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPD"
BASE COUNT           88 a           55 c           49 g           94 t
ORIGIN      
        1 tcatctggta aaagtttcac tgtgtttgtt agaggggaat tatgcctatc ttcaaatatt
       61 acctgcccgg gttgctccag accataaatt ttccaataaa aattatcaaa aaaatcctta
      121 gcggataatt gactcagttc gaaatggcta tcacaagtaa atacagtacg tttatgagta
      181 acagttcgat taggatcgaa aaactgaaca acccattttt cactttcttg tacgcgcttt
      241 attttcaacc ctaacgccat ttggtggttt aatgtgcatg ctgata
//
LOCUS       DAZPAC010000709          284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.732, whole genome shotgun sequence.
ACCESSION   DAZPAC010000709 DAZPAC010000000
VERSION     DAZPAC010000709.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.732"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..133
                     /locus_tag="ACHUPM_005032"
     CDS             <1..133
                     /locus_tag="ACHUPM_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR0872504.1"
                     /translation="SRDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDISLLRYMPA"
BASE COUNT           75 a           58 c          101 g           50 t
ORIGIN      
        1 gagtcgtgac ccgttcgaat gcgtgctgtg tggcggccgg atggtatacc gccgggccat
       61 tgcggggctg aatgtggagg ggctgaagaa aaacgcgcgg gatatcagtc tgctgaggta
      121 tatgccggcc tgacacaggc taagtgtgcc cgggaatcgg tgcaagtata aaaaacagcc
      181 tgttgcggta taagaatcga ccatgcagag agagaaaaca ccggagtggt ggggaaaaac
      241 agaaaggctc acgtggcata aggcgcggac aggggtatcg ctta
//
LOCUS       DAZPAC010000710          283 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.733, whole genome shotgun sequence.
ACCESSION   DAZPAC010000710 DAZPAC010000000
VERSION     DAZPAC010000710.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.733"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>283
                     /locus_tag="ACHUPM_005033"
     CDS             <1..>283
                     /locus_tag="ACHUPM_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR0872505.1"
                     /translation="RLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQVHH
                     FAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGA"
BASE COUNT           72 a           55 c           87 g           69 t
ORIGIN      
        1 cgacttatca gtggaaaaac acagaacgat caggatatat gcgccctggc aaaagagtat
       61 gagatcgcca tgctgttggt gactaaaggt gcagaagggg tggtggtctg ttatcgagga
      121 caagttcacc attttgctgg aatgtctgtg aattgtgtcg atagcacggg ggcgggagat
      181 gcgttcgttg ccgggttact cacaggtctg tcctctacgg gattatctac agatgagaga
      241 gaaatgcgac gaattatcga tctcgctcaa cgttgcggag cgc
//
LOCUS       DAZPAC010000711          283 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.734, whole genome shotgun sequence.
ACCESSION   DAZPAC010000711 DAZPAC010000000
VERSION     DAZPAC010000711.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.734"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            200..>283
                     /locus_tag="ACHUPM_005034"
     CDS             200..>283
                     /locus_tag="ACHUPM_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0872506.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIV"
BASE COUNT           75 a           70 c           77 g           61 t
ORIGIN      
        1 cgtagtatcc acaccaagcc ggcgttatcc ggtgaggcgc aatgttgcgg gggcttaatc
       61 cctggtggca ttggttgctg gaaagagaaa acccccgcac gttgagaggt aaaacctgac
      121 aacagaacgg gggctaatct tgaaccatca acaattcaag aatagccgca aaatgttgtc
      181 attacaacaa ggcggctata tgacgctcgc gaagtttgcc atgattttct ggcacgacct
      241 ggcagcaccg atcctggcgg ggattattac cgcagcgatt gtc
//
LOCUS       DAZPAC010000712          281 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.735, whole genome shotgun sequence.
ACCESSION   DAZPAC010000712 DAZPAC010000000
VERSION     DAZPAC010000712.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.735"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
BASE COUNT           95 a           51 c           51 g           84 t
ORIGIN      
        1 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
       61 ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag
      121 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      181 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      241 gttaaatata ggaaaaatta cttaatctat cttaataacg g
//
LOCUS       DAZPAC010000713          281 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.736, whole genome shotgun sequence.
ACCESSION   DAZPAC010000713 DAZPAC010000000
VERSION     DAZPAC010000713.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.736"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>281)
                     /gene="ospI"
                     /locus_tag="ACHUPM_005035"
     CDS             complement(<1..>281)
                     /gene="ospI"
                     /locus_tag="ACHUPM_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFR0872507.1"
                     /translation="NGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSELA
                     ATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFF"
BASE COUNT           81 a           78 c           37 g           85 t
ORIGIN      
        1 tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc tcttactcca
       61 taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc atccccacca
      121 gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc catatcacca
      181 agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc ctcctctgta
      241 tgtgcctcat atccagcagt tccctgtaac gacaccccat t
//
LOCUS       DAZPAC010000714          279 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.737, whole genome shotgun sequence.
ACCESSION   DAZPAC010000714 DAZPAC010000000
VERSION     DAZPAC010000714.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.737"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..67
                     /gene="mxiH"
                     /locus_tag="ACHUPM_005036"
     CDS             <1..67
                     /gene="mxiH"
                     /locus_tag="ACHUPM_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HFR0872508.1"
                     /translation="AQSNTVKVIKDVDAAIIQNFR"
     gene            80..>279
                     /gene="mxiI"
                     /locus_tag="ACHUPM_005037"
     CDS             80..>279
                     /gene="mxiI"
                     /locus_tag="ACHUPM_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFR0872509.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKL"
BASE COUNT          102 a           44 c           54 g           79 t
ORIGIN      
        1 cgcgcaatcc aatacagtga aagtgattaa ggatgttgat gctgcaatta ttcaaaactt
       61 cagataatag ggagcattca tgaattacat ttatccagtc aatcaggttg atattatcaa
      121 agccagtgat tttcaatctc aagagatatc aagtctggaa gatgtcgtgt cggctaaata
      181 tagtgatatt aagatggata cagatattca agtatcacaa ataatggaga tggtaagcaa
      241 tccagaatca ttaaacccag aatctttggc caagttaca
//
LOCUS       DAZPAC010000715          279 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.738, whole genome shotgun sequence.
ACCESSION   DAZPAC010000715 DAZPAC010000000
VERSION     DAZPAC010000715.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.738"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(50..>279)
                     /gene="trbI"
                     /locus_tag="ACHUPM_005038"
     CDS             complement(50..>279)
                     /gene="trbI"
                     /locus_tag="ACHUPM_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR0872510.1"
                     /translation="QTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHA
                     VILVSPAVVQGAPDITREIQQDIARRMRAEP"
BASE COUNT           51 a           88 c           73 g           67 t
ORIGIN      
        1 gcggccacac tctgccccca tatcagcagg gcaatcagcc cccggcatct catggttccg
       61 ctctcattcg ccgggcaata tcctgctgga tttcccgggt gatatccgga gccccctgta
      121 ccacggcagg cgacaccaga atgaccgcgt ggtgtttctg ctgccacgcc tgcaggctgg
      181 cttccagtgc cgtgttaaaa cgcgctgaaa gggcttttga ctgtgcttct gacagttgtt
      241 tctgactggc gctgtcaaaa aatgcatcca cagtctgtt
//
LOCUS       DAZPAC010000716          277 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.739, whole genome shotgun sequence.
ACCESSION   DAZPAC010000716 DAZPAC010000000
VERSION     DAZPAC010000716.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.739"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>277
                     /gene="virF"
                     /locus_tag="ACHUPM_005039"
     CDS             <1..>277
                     /gene="virF"
                     /locus_tag="ACHUPM_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HFR0872511.1"
                     /translation="YSEEKRGLNKKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACL
                     LSAVSDEEALYTSISIASSLSFSDQIRKIVEKNIEKRWRLSDISNNLN"
BASE COUNT           88 a           41 c           55 g           93 t
ORIGIN      
        1 ctattctgag gagaaaaggg ggttaaacaa aaaaatattc ctcctctctg aggaggaggt
       61 ttctatcgat ttgttcaaat ctataaaaga gatgcctttc ggcaaaagaa agatctatag
      121 tttagcttgc cttttatcag ctgtttctga tgaggaagct ttatatactt cgatatcgat
      181 agcttcttct cttagttttt ctgatcagat aaggaagatt gttgaaaaaa acatcgagaa
      241 gagatggcgt ctttctgata tttcaaataa cttgaat
//
LOCUS       DAZPAC010000717          277 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.740, whole genome shotgun sequence.
ACCESSION   DAZPAC010000717 DAZPAC010000000
VERSION     DAZPAC010000717.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.740"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>277)
                     /locus_tag="ACHUPM_005040"
     rRNA            complement(<1..>277)
                     /locus_tag="ACHUPM_005040"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           50 a           85 c           63 g           79 t
ORIGIN      
        1 gcgtgccttc tcccgaagtt acggcaccat tttgcctagt tccttcaccc gagttctctc
       61 aagcgccttg gtattctcta cctgaccacc tgtgtcggtt tggggtacga tttgatgtta
      121 cctgatgctt agaggctttt cctggaagca gggcatttgt tgcttcagca ccgtagtgcc
      181 tcgtcatcac gcctcagcct tgattttccg gatttgcctg gaaaaccagc ctacacgctt
      241 aaaccgggac aaccgtcgcc cggccaacat agccttc
//
LOCUS       DAZPAC010000718          274 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.741, whole genome shotgun sequence.
ACCESSION   DAZPAC010000718 DAZPAC010000000
VERSION     DAZPAC010000718.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.741"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>274)
                     /locus_tag="ACHUPM_005041"
     CDS             complement(<1..>274)
                     /locus_tag="ACHUPM_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0872512.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYA"
BASE COUNT           66 a           87 c           52 g           69 t
ORIGIN      
        1 gcatattcaa acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac
       61 tgtagcatca gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca
      121 ccattacgct cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat
      181 tcaatcaatg cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg
      241 tcatacccca tcaccctcag ggctttattt acag
//
LOCUS       DAZPAC010000719          272 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.742, whole genome shotgun sequence.
ACCESSION   DAZPAC010000719 DAZPAC010000000
VERSION     DAZPAC010000719.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.742"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>272
                     /gene="hlyE"
                     /locus_tag="ACHUPM_005042"
     CDS             <1..>272
                     /gene="hlyE"
                     /locus_tag="ACHUPM_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR0872513.1"
                     /translation="NKYLDQVIPWQTFDETIKELSRFKQEYSQAASVLVGDIKTLLMD
                     SQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKASAQKDILI"
BASE COUNT           85 a           53 c           58 g           76 t
ORIGIN      
        1 ataataaata tctcgatcag gtcatcccct ggcagacctt tgatgaaacc ataaaagagt
       61 taagtcgctt taaacaggag tattcacagg cagcctccgt tttagtcggc gatattaaaa
      121 ccttacttat ggatagccag gataagtatt ttgaagcaac ccaaacagtg tatgaatggt
      181 gtggtgttgc gacgcaattg ctcgctgcgt atattttgct atttgatgag tacaatgaga
      241 agaaagcatc cgcccagaaa gacattctca tt
//
LOCUS       DAZPAC010000720          259 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.744, whole genome shotgun sequence.
ACCESSION   DAZPAC010000720 DAZPAC010000000
VERSION     DAZPAC010000720.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.744"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            2..>259
                     /gene="istB"
                     /locus_tag="ACHUPM_005043"
     CDS             2..>259
                     /gene="istB"
                     /locus_tag="ACHUPM_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR0872514.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNACLKYQATPEGLRYPASRGLRAEQ"
BASE COUNT           74 a           61 c           76 g           48 t
ORIGIN      
        1 tatgagtgat aatctgttaa acaaactgac ccaactgaaa ctcccggcga tggccgggag
       61 cctgatacgg caacgggaaa cgcctcagac ttacgacgaa ctgtcgttcg aggaaaggct
      121 gacactgctg gttgatgacg agcttctgag cagagaaaac agtcgtgtgg cacggttgag
      181 aaaaaatgcc tgcctgaaat atcaggcaac accggaaggg ctgcgttatc cggcctcaag
      241 aggtctgcgg gctgaacag
//
LOCUS       DAZPAC010000721          252 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.745, whole genome shotgun sequence.
ACCESSION   DAZPAC010000721 DAZPAC010000000
VERSION     DAZPAC010000721.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.745"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..66
                     /locus_tag="ACHUPM_005044"
     CDS             <1..66
                     /locus_tag="ACHUPM_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mxiK"
                     /protein_id="HFR0872515.1"
                     /translation="RLNEGMQYAKRHFTGIQTSCL"
     gene            77..>252
                     /gene="mxiN"
                     /locus_tag="ACHUPM_005045"
     CDS             77..>252
                     /gene="mxiN"
                     /locus_tag="ACHUPM_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HFR0872516.1"
                     /translation="MKRIEKELCKTIKDRDTESKKKAICVIKDATKKAESLRIDAVCD
                     GYQIGIQTAFEHII"
BASE COUNT           96 a           30 c           56 g           70 t
ORIGIN      
        1 cgattaaatg aaggtatgca atatgcaaaa aggcacttta ccggtatcca gacatcatgc
       61 ctatgatggt gttgttataa agagaattga aaaggaatta tgtaaaacca taaaagatag
      121 agacactgag agtaagaaga aagcaatttg tgttataaag gatgctacga aaaaagctga
      181 atcactgcgt atagatgctg tatgtgatgg ttatcaaatt ggtatacaga ctgcttttga
      241 gcatattatt ga
//
LOCUS       DAZPAC010000722          249 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.746, whole genome shotgun sequence.
ACCESSION   DAZPAC010000722 DAZPAC010000000
VERSION     DAZPAC010000722.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.746"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
BASE COUNT           71 a           52 c           29 g           97 t
ORIGIN      
        1 catggtttat tcctcttcat ctgaattgat ttacattagg ttggttattt tctcataccc
       61 acaactatat tttctctctg aatttattac gtgcaagaca atcgcagcac tctaactaac
      121 ttcgatgcaa tttgatgttc agaaaaatcc ctcattagat tattatcatg aaatacagct
      181 aatcatcttt agcttatata tttctcaatg tattcttttg aaaggatccg tgccctttca
      241 aactcttca
//
LOCUS       DAZPAC010000723          245 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.747, whole genome shotgun sequence.
ACCESSION   DAZPAC010000723 DAZPAC010000000
VERSION     DAZPAC010000723.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.747"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            82..>245
                     /locus_tag="ACHUPM_005046"
     CDS             82..>245
                     /locus_tag="ACHUPM_005046"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0872517.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQ"
BASE COUNT           75 a           60 c           54 g           56 t
ORIGIN      
        1 gcctgaaccg tctggtcaga atctgacgaa ttagacaaag tggtgtccac caaataagta
       61 gtgggaacca aagtgtcaga tatgcagaaa aatgtgactc ccggcaggcg aaaaggctgc
      121 cctaattatc ctcccgaatt taaacagcag ctcgttgctg cctcctgtga acccgggata
      181 tccatctcaa aacttgctct tgaaaatggc attaacgcca atctgttgtt caaatggcga
      241 caaca
//
LOCUS       DAZPAC010000724          244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.748, whole genome shotgun sequence.
ACCESSION   DAZPAC010000724 DAZPAC010000000
VERSION     DAZPAC010000724.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.748"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
BASE COUNT           92 a           54 c           27 g           71 t
ORIGIN      
        1 aattgcgggt ataaaataca tctattttca aataaaataa agcatactcc aattacaacg
       61 agtgactaaa ataaaaatat catccaagtc aatcactgtt tgctctgcaa aatatgtcag
      121 ccttctcaaa cttaccagca cttaaatatt taagcacaca atcattcctt gagggagttc
      181 acttatttca agagaaacat ttcgtcttcc cacacaacta atccgcccaa tatagctaaa
      241 tttg
//
LOCUS       DAZPAC010000725          244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.749, whole genome shotgun sequence.
ACCESSION   DAZPAC010000725 DAZPAC010000000
VERSION     DAZPAC010000725.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.749"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..233)
                     /locus_tag="ACHUPM_005047"
     CDS             complement(<1..233)
                     /locus_tag="ACHUPM_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR0872518.1"
                     /translation="MATTCSVILILESFDVYFGKESVFLERGSSVLVDSSSRDFFLTY
                     PERVIVADFGAEFISRYLKANNLRDISDCREYLS"
BASE COUNT           90 a           62 c           31 g           61 t
ORIGIN      
        1 gatagatatt ccctacaatc agaaatatcc cttaagttat tagctttcaa atagcgacta
       61 ataaactcag cgccaaaatc cgccactatc actctttcag gatatgtcag gaaaaaatct
      121 ctagagctag agtcgacaag tacagatgaa cctctctcca gaaacacact ctcttttccg
      181 aaataaacat caaaggactc caaaatcaat ataactgaac atgtagttgc catatcatcc
      241 accc
//
LOCUS       DAZPAC010000726          241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.750, whole genome shotgun sequence.
ACCESSION   DAZPAC010000726 DAZPAC010000000
VERSION     DAZPAC010000726.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.750"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>241
                     /locus_tag="ACHUPM_005048"
     CDS             <1..>241
                     /locus_tag="ACHUPM_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0872519.1"
                     /translation="LFLDELPEFERRTLDALREPIESGQIHLSRTRAKITYPARFQLV
                     AAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFL"
BASE COUNT           59 a           72 c           53 g           57 t
ORIGIN      
        1 tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc ttgcgagagc
       61 cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc tatccagccc
      121 gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag ggaaaccata
      181 accgctgcac gccagaacag acattacgtt atctcaaccg gctctcgggg ccctttctcg
      241 a
//
LOCUS       DAZPAC010000727          240 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.751, whole genome shotgun sequence.
ACCESSION   DAZPAC010000727 DAZPAC010000000
VERSION     DAZPAC010000727.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.751"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..>240
                     /locus_tag="ACHUPM_005049"
     rRNA            <1..>240
                     /locus_tag="ACHUPM_005049"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           61 a           55 c           80 g           44 t
ORIGIN      
        1 ggacgaagac tgacgctcag gtgcgaaagc gtggggagca aacaggatta gataccctgg
       61 tagtccacgc cgtaaacgat gtcgacttgg aggttgtgcc cttgaggcgt ggcttccgga
      121 gctaacgcgt taagtcgacc gcctggggag tacggccgca aggttaaaac tcaaatgaat
      181 tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgatgcaac gcgaagaacc
//
LOCUS       DAZPAC010000728          232 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.denovo.754, whole genome shotgun sequence.
ACCESSION   DAZPAC010000728 DAZPAC010000000
VERSION     DAZPAC010000728.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.denovo.754"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..>232)
                     /gene="ospZ"
                     /locus_tag="ACHUPM_005050"
     CDS             complement(<1..>232)
                     /gene="ospZ"
                     /locus_tag="ACHUPM_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HFR0872520.1"
                     /translation="CPEDFQPISRDEASTPEGSWLTVISGKRPMGQFSVDSLYHPDLH
                     ALCELPEISCKIFPKENSDFLYIIVVFRNDSPQ"
BASE COUNT           74 a           49 c           42 g           67 t
ORIGIN      
        1 ttggggagaa tcatttctga atacaacaat tatatacaaa aaatcactat tttctttagg
       61 gaagatctta caactaatct ctgggagctc acataatgca tgtaagtcag gatggtacaa
      121 actatccaca gaaaattgcc ccataggacg ttttccagat ataactgtca accagcttcc
      181 ttccggtgta ctggcttcgt ctctggaaat aggctgaaaa tcttccggac at
//
LOCUS       DAZPAC010000729         7676 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.guided.197, whole genome shotgun sequence.
ACCESSION   DAZPAC010000729 DAZPAC010000000
VERSION     DAZPAC010000729.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.guided.197"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            1..652
                     /locus_tag="ACHUPM_005051"
                     /pseudo
     CDS             1..652
                     /locus_tag="ACHUPM_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /gene="prfH"
                     /locus_tag="ACHUPM_005052"
     CDS             649..1263
                     /gene="prfH"
                     /locus_tag="ACHUPM_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR0872521.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHUPM_005053"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHUPM_005053"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0872522.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="ACHUPM_005054"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="ACHUPM_005054"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0872523.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="ACHUPM_005055"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="ACHUPM_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR0872524.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..5291
                     /gene="crl"
                     /locus_tag="ACHUPM_005056"
     CDS             4890..5291
                     /gene="crl"
                     /locus_tag="ACHUPM_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR0872525.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="ACHUPM_005057"
     CDS             complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="ACHUPM_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR0872526.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6673..>7676
                     /gene="proB"
                     /locus_tag="ACHUPM_005058"
     CDS             6673..>7676
                     /gene="proB"
                     /locus_tag="ACHUPM_005058"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR0872527.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGNSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     "
BASE COUNT         1864 a         1894 c         1994 g         1924 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaaatttgc caatttggta
     4861 aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     5041 gtgagttctg gggctggtgg atggagcttg aagcgcagga atcccgtttt acctacagtt
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagacactg
     5161 aagtggttga acgactggag cacaccctac gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgta tcacatccgg caatatccat taaaactgat
     5341 aggtcatgcc aaccgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     5401 gttggttgat tttataatca acaaacgctg acatattttt gttgaaataa tacgtagcac
     5461 cgacgtcgat ataattgacc agatcttcat caccgatacc ttcaatatct ttccctttcg
     5521 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     5581 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgtgtttcag
     5641 aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
     5701 ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
     5761 tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
     5821 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     5881 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     5941 tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga atcgccacca aattccggga
     6001 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag
     6061 aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttctgc tgtgcagtat
     6121 cactctccgc tttatttccg gcaaactccg cttcccaccg accataacca gtcagttgat
     6181 cgttaatttg tgtttcacct ttaaaaccaa aacggatata actctggtcg ccatctttac
     6241 tggcgttatc actcatataa tgcatagctt taactttgcc atagacatcc agtttattac
     6301 cgtctttatt atatatttct gcagcctgta cagatgcaga tgccacaatg cccatcacca
     6361 ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc
     6421 agcgggagag ccccgttgaa aacaggaaag tttttaacct gagattgtta aagatatatt
     6481 acagattaat aatattctta aaatgtggta atttattaaa tctgtaataa aagcgtaaac
     6541 aactgccgct aggcttgctg atcccgcgca acaaaacgcc atgctttgct cgcagatggt
     6601 tggcaaccga cgacagtcct gctaaaacgt tcgtttgata tcatttttcc taaaattgaa
     6661 tggcagagaa tcatgagtga cagccagacg ctggtggtaa aactcggcac cagtgtgcta
     6721 acaggcggat cgcgccgtct gaaccgtgcc catattgttg aacttgttcg ccagtgcgcg
     6781 cagttacatg ccgccgggca tcggattgtt attgtgacgt cgggcgcgat cgccgccgga
     6841 cgtgagcacc tgggttaccc ggaactgcca gcgactatcg cctcgaaaca actgctggca
     6901 gcggtagggc agagtcgact gattcaactg tgggaacagc tgttttcgat ttatggcatt
     6961 cacgtcgggc aaatgctgct gacccgtgct gatatggaag accgtgaacg cttcctgaac
     7021 gcccgcgaca ccctgcgtgc gttgctcgat aacaatatcg ttccggtaat caatgagaac
     7081 gatgctgtcg ctacggcaga gattaaagtc ggcgataacg ataacctttc tgcgctggcg
     7141 gcgattctgg cgggtgccga taaactgttg ctgctgaccg atcaaaaagg tttgtatacc
     7201 gctgacccgc gcagcaatcc gcaggcagaa ctgattaaag atgtttacgg cattgatgac
     7261 gcactgcgcg cgattgccgg taacagcgtt tcaggcctcg gaactggcgg catgagtacc
     7321 aaattgcagg ccgctgacgt ggcttgccgt gcgggtatcg acaccattat tgccgcgggc
     7381 agcaagccgg gcgttattgg tgatgtgatg gaaggcattt ccgtcggtac gctgttccat
     7441 gcccaggcga ctccgcttga aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt
     7501 gaaatcacgg tagatgaagg ggcaactgcc gccattctgg aacgcggcag ctccctgttg
     7561 ccgaaaggca ttaaaagcgt gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc
     7621 aacctcgaag gccgcgatat cgcccacggc gtcagtcgtt acaacagcga tgcatt
//
LOCUS       DAZPAC010000730         5546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.guided.254, whole genome shotgun sequence.
ACCESSION   DAZPAC010000730 DAZPAC010000000
VERSION     DAZPAC010000730.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.guided.254"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            561..800
                     /locus_tag="ACHUPM_005059"
     CDS             561..800
                     /locus_tag="ACHUPM_005059"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR0872528.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(988..1965)
                     /gene="intI2"
                     /locus_tag="ACHUPM_005060"
                     /pseudo
     CDS             complement(988..1965)
                     /gene="intI2"
                     /locus_tag="ACHUPM_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2297..2770
                     /gene="dfrA1"
                     /locus_tag="ACHUPM_005061"
     CDS             2297..2770
                     /gene="dfrA1"
                     /locus_tag="ACHUPM_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR0872529.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2865..3389
                     /gene="sat2"
                     /locus_tag="ACHUPM_005062"
     CDS             2865..3389
                     /gene="sat2"
                     /locus_tag="ACHUPM_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0872530.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3458..3955
                     /locus_tag="ACHUPM_005063"
     CDS             3458..3955
                     /locus_tag="ACHUPM_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872531.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            4016..4387
                     /locus_tag="ACHUPM_005064"
     CDS             4016..4387
                     /locus_tag="ACHUPM_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872532.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            4797..5174
                     /locus_tag="ACHUPM_005065"
     CDS             4797..5174
                     /locus_tag="ACHUPM_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872533.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            5197..5349
                     /locus_tag="ACHUPM_005066"
                     /pseudo
     CDS             5197..5349
                     /locus_tag="ACHUPM_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5405..>5546)
                     /locus_tag="ACHUPM_005067"
     CDS             complement(5405..>5546)
                     /locus_tag="ACHUPM_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR0872534.1"
                     /translation="NSLFDQLYGQDGHRGVNHPKGLGELQVSREDMEGWAERVVREQF
                     TH"
BASE COUNT         1553 a         1245 c         1268 g         1480 t
ORIGIN      
        1 ggatcggtca actgatcctt aactgatcgg cattacaccg gtcggcagcc ctgtgggcgg
       61 acaaaatagt tgggaactgg gaggggtgga aatggagttt ttaaggatta tttagggaag
      121 agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg
      181 acaaaatagt ttggaactag atttcactta tctggttaac taatctagct aacctgccgc
      241 tcagcttagt acgatcctat atccaactga tcataatcct ttcatcagta tcccaaatta
      301 aatccccctc ctagactgcc tcaccaacac tttacagtga tcccgtattt ccggacaggt
      361 gattgatttt tttcgcttgt acaggtgtta agccacagtt atcttgatgt ggacattatg
      421 cattgtaata gcccattaac atgcgctaag agcataaatt aatatacagt aacaagcatt
      481 acgaggtgct aatgtttgga aagcagcctc ttccaactac aataataagt taactatcct
      541 ccacccacaa ataattacaa atgacccctt tccgctataa ctccgatctc accagcggct
      601 cgctacaaac ccgagagtgt cgcattatta ctggcttgtt actccaagag cttgatgaag
      661 ctgcctggga taaagccatg tataaggaaa atgtgcttca gaaacgcacg caatctacgg
      721 taagacgtat ttcttcagcg cttagaaaac gcctagaaca cctaagctct gacttttggg
      781 cttttgcgtt tttatgttag gcattgtctg gcaactggag gaagtgtaat gaccatgaat
      841 ggtcactgtg attgacagta tcttgcttaa acttatctga acaatggtag aactggctca
      901 tatttatccc ctctatgatt gtttatcttt ttcaccataa aaagtatcag gggctactca
      961 gggctacata aagatattta tctactttta ctgattgata agtagcatca gtccatccgc
     1021 aggactggtg gtgccggcaa aatgctgacc caacacatgc gtatagattt gcgtggtctt
     1081 aacatcgtta tgccctaaga gttcttgcac agtgcgaata tcacgccccg cttgtaatag
     1141 atgcgtagca aacgagtgac gaaatgtatg acaagtgaca cgcttgctaa cgatgcctgc
     1201 tttttgtacg gctgccttca atgcctttcg cgcaacggag tcatgcagat gatggcggca
     1261 taatttgccg ttatacgggt ggttgcagag cgtgctggag ggaaagacaa acatccacgc
     1321 cgcttgtcga taagcagaag ggtatttgtg atctaaagca aaaggcagcg atggccctac
     1381 gccttgtaag ttgtcgtctt gctgaataag ccgcgcttgc tcaatgagtt attttattgc
     1441 tgggattagg cgcgtgggca gtaggctgtt tctgcttttc ccacccttac cgtcatgcac
     1501 agtgatgcag ccattatcaa aatcaaaatc tttaacccgc aaacgcaagc attcattaat
     1561 gcgcaaacct gcaccataca gcagcgtaaa aataacttgg ttgcgagtat ccataacctg
     1621 caaaatgcgt tgcacttcat ttgcagagat aacagagggt agccgtctag gcttgcttgc
     1681 agggatataa tcaatatcgc ccaacggctg ttgtaaaaac ctgttgtaca aaaaagctag
     1741 ggcatttaaa gcgattttct gcgtgtttat ggctacatgt ctgctgtttg ctaagctgga
     1801 taaaaacagc ctgacctctt cactgcccat ggtctgagga tgacgttttt tgtgaaacag
     1861 aataaaacgc ttaatccagt gcaggtaagt tttttcagtt ttcagcgcat aacctttttg
     1921 tcgcatatcc gtgcgtatag aatttaaaaa tggactgtta gacataaaac gctccttgtc
     1981 ttgcaactgt ctgcctatac agcctattct agctgggatt taaaaaagtg cctgtttttt
     2041 acgcctagag atgcttgttt accggtagag ttttaattta atgctaaata aattaaaatg
     2101 ttatgagttc tttgggtgag ataatgtgca tcgtgcaagc aggatagacg gcatgcacga
     2161 tttgtaataa cagagtgtct tgtattttta aagaaagtct atttaataca agtgattata
     2221 ttaattaacg gtaagcatca gcgggtgaca aaacgagcat gcttactaat aaaatgttaa
     2281 cctctgagga agaattgtga aactatcact aatggtagct atatcgaaga atggagttat
     2341 cgggaatggc cctgatattc catggagtgc caaaggtgaa cagctcctgt ttaaagctat
     2401 tacctataac caatggctgt tggttggacg caagactttt gaatcaatgg gagcattacc
     2461 caaccgaaag tatgcggtcg taacacgttc aagttttaca tctgacaatg agaacgtatt
     2521 gatctttcca tcaattaaag atgctttaac caacctaaag aaaataacgg atcatgtcat
     2581 tgtttcaggt ggtggggaga tatacaaaag cctgatcgat caagtagata cactacatat
     2641 atctagaata gacatcgagc cggaaggtga tgtttacttt cctgaaatcc ccagcaattt
     2701 taggccagtt tttacccaag acttcgcctc taacataaat tatagttacc aaatctggca
     2761 aaagggttaa caagtggcag caacggattc gcaaacctgt cacgcctttt gtaccaaaag
     2821 ccgcgccagg tttgcgatcc gctgtgccag gcgttaggcg tcatatgaag atttcggtga
     2881 tccctgagca ggtggcggaa acattggatg ctgagaacca tttcattgtt cgtgaagtgt
     2941 tcgatgtgca cctatccgac caaggctttg aactatctac cagaagtgtg agcccctacc
     3001 ggaaggatta catctcggat gatgactctg atgaagactc tgcttgctat ggcgcattca
     3061 tcgaccaaga gcttgtcggg aagattgaac tcaactcaac atggaacgat ctagcctcta
     3121 tcgaacacat tgttgtgtcg cacacgcacc gaggcaaagg agtcgcgcac agtctcatcg
     3181 aatttgcgaa aaagtgggca ctaagcagac agctccttgg catacgatta gagacacaaa
     3241 cgaacaatgt acctgcctgc aatttgtacg caaaatgtgg ctttactctc ggcggcattg
     3301 acctgttcac gtataaaact agacctcaag tctcgaacga aacagcgatg tactggtact
     3361 ggttctcggg agcacaggat gacgcctaac aattcattca agccgacacc gcttcgcggc
     3421 gcggcttaat tcaggagtta gagagctggg gaagactatg cgcgatctgt tgaaggtggt
     3481 tctaagcctc gtacttgcga tggcatcggg gcaggcactt gctgacctgc caattgtttt
     3541 agtggatgaa gctcgtcttc cctatgacta ctccccatcc aactacgaca tttctccaag
     3601 caactacgac aactccataa gcaattacga caatagtcca tcaaattacg acaactctga
     3661 gagcaactac gataatagtt catccaatta cgacaatagt cgcaacggaa atcgtaggct
     3721 tatatatagc gcaaatgggt ctcgcacttt cgccggctac tacgtcattg ccaacaatgg
     3781 gacaacgaac ttcttttcca catctggcaa aaggatgttc tacaccccaa aaggggggcg
     3841 cggcgtctat ggcggcaaag atgggagctt ctgcggggca ttggtcgtca taaatggcca
     3901 attttcgctt gccctgacag ataacggcct gaagatcatg tatctaagca actagcctgc
     3961 tctctaataa aatgttaggc ctcaacatct agtcgcaagc tgaggggaac cactagtgtc
     4021 atacgaacct ccaagagacg gttacacaaa cgggtacatt gttgatgtca tgtatgacaa
     4081 tcgcccaagt aagtatccag ctgtgttcag aacgtacgtc cgaattccag actatgtcga
     4141 actagataac gcggaggagc tggccgacgc aatcattcga gaggttgagt taaaggaggc
     4201 atggctcaca agggtcttgg ggaactcagc cgatcctgac gcttctgtgg gcaagtggga
     4261 ccggcgccta gcgcagtgcg gtggtattga tgagatgtca agtggggtgc aggcaacata
     4321 cttcagtcca aacggtcagc tggtcggctt agtggcatac attgatgccg aatggatgga
     4381 tgaatagctc tgacgttacg attcaggtga gacaaccaga tgggcattgc gtacaagctc
     4441 agttgccacc gatgtggcta cgagtctgat cttctttacc taggccaagg tatggcgatg
     4501 cttcctgagc aaatagtcgg aacgtgcaca tgctgcgaca atctaacgac gatttctata
     4561 aatgaaagct acggagtatg ctcactttgt ggggctaagg atcgtgtgat atttacaagc
     4621 tatcagcatg aggcaaggcc aagaaatccc tgctatctag agagaagttt caaatatcaa
     4681 tgcccaagat gccatgcctt cagtatggag cctccggatc taccggagat gctttgggac
     4741 taatgctccg ctttgcctcg ggtatccgtt cgacctatgt ttaagaggaa acgcacgtgt
     4801 ctgccgccaa agctcttgcc tatgcacttt gtgacaagtt ggaaagcaaa aaagttgatg
     4861 aagtcgtacg aaatcttgtt tctagcggct ggaatattag ggaagtggcc tgggaagctt
     4921 cgtcctttga tcatgaaatg ccctactttg acacggggcg ggacttcgat attggatttc
     4981 ctgacacgct gtcatccaag attgagtttt tgaccgcgaa tggatcaatc gtccaagcac
     5041 gtgctcgcct gcacttcaag caggctctaa tttttgccaa ggcgtccaaa catgtcgccg
     5101 cgctcaagaa ctcattggat tactactatg gatcgtctgt aatcccccct caaaaccacg
     5161 gaggctattt gtagaaaatt ccattaatat gaactcaagg tgttccctac ggacgaatca
     5221 gcgatgaagg tggtgtacct ggctatccag gcggcgacca agaaatggac gatgccgatc
     5281 cgcaactgga agccggccat gaaccgtttt atgatcgaat ttggtgaccg tttaaatggc
     5341 cacctttaac tgaccggcaa aagcacttac acaaaagggt ttacaggctc tttcagtcat
     5401 tcctttaatg cgtaaattgc tctctaacca ctctctcagc ccacccttcc atatcctctc
     5461 tcgatacttg cagctccccc aacccctttg gatgattcac ccctctatgc ccgtcttgtc
     5521 catataattg atcaaacaaa ctattt
//
LOCUS       DAZPAC010000731         3842 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.guided.320, whole genome shotgun sequence.
ACCESSION   DAZPAC010000731 DAZPAC010000000
VERSION     DAZPAC010000731.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.guided.320"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHUPM_005068"
     CDS             <1..196
                     /locus_tag="ACHUPM_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFR0872535.1"
                     /translation="AGARTRATAHERTPQQERQKAAREAERGREAWTLGQGMKKPVAG
                     CYGRLTRWKGGGDVVYMALL"
     gene            complement(228..878)
                     /gene="tetR(A)"
                     /locus_tag="ACHUPM_005069"
     CDS             complement(228..878)
                     /gene="tetR(A)"
                     /locus_tag="ACHUPM_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFR0872536.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            984..2183
                     /gene="tet(A)"
                     /locus_tag="ACHUPM_005070"
     CDS             984..2183
                     /gene="tet(A)"
                     /locus_tag="ACHUPM_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFR0872537.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            complement(2150..2338)
                     /locus_tag="ACHUPM_005071"
                     /pseudo
     CDS             complement(2150..2338)
                     /locus_tag="ACHUPM_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(2382..2771)
                     /locus_tag="ACHUPM_005072"
     CDS             complement(2382..2771)
                     /locus_tag="ACHUPM_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872538.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            2989..3429
                     /locus_tag="ACHUPM_005073"
     CDS             2989..3429
                     /locus_tag="ACHUPM_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR0872539.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(3488..>3842)
                     /locus_tag="ACHUPM_005074"
     CDS             complement(3488..>3842)
                     /locus_tag="ACHUPM_005074"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872540.1"
                     /translation="TSLLHHLYITFTSFLQLIRHFHPLCHLYITPFLNVLTAGFPAAP
                     ATLHGHAINCVPVHRFRMGRTPETGLTPAEWCEFPRYALVRILMHAVKTEPARFWRVF
                     GWFVACFTGFPSETP"
BASE COUNT          808 a         1102 c         1091 g          841 t
ORIGIN      
        1 ggccggcgcg cggacccgcg ccacggccca cgagcggacg ccgcagcagg agcgccagaa
       61 ggccgccaga gaggccgagc gcggccgtga ggcttggacg ctagggcagg gcatgaaaaa
      121 gcccgtagcg ggctgctacg ggcgtctgac gcggtggaaa gggggagggg atgttgtcta
      181 catggctctg ctgtagtgag tgggttgcgc tccggcagcg gtcctgatca atcgtcaccc
      241 tttctcggtc cttcaacgtt cctgacaacg agcctccttt tcgccaatcc atcgacaatc
      301 accgcgagtc cctgctcgaa cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg
      361 gcccgcaaca gcggcgagag cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg
      421 ctgtcgccgg cctgctcctc aagcacggcc ccaacagtga agtagctgat tgtcatcagc
      481 gcattgacgg cgtccccggc cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg
      541 gccgtttcca tctgcggtgc gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg
      601 taggcgagca gcgcctgcct gaagctgcgg gcattcccga tcagaaatga gcgccagtcg
      661 tcgtcggctc tcggcaccga atgcgtatga ttctccgcca gcatggcttc ggccagtgcg
      721 tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg aacccccaac
      781 cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa caggtccagg
      841 gcggcacgga tcactgtatt cggctgcaac tttgtcatgc ttgacacttt atcactgata
      901 aacataatat gtccaccaac ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc
      961 ggaggctggt ccggaggcca gacgtgaaac ccaacagacc cctgatcgta attctgagca
     1021 ctgtcgcgct cgacgctgtc ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc
     1081 gcgatctggt tcactcgaac gacgtcaccg cccactatgg cattctgctg gcgctgtatg
     1141 cgttgatgca atttgcctgc gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc
     1201 ggccggtctt gctcgtctcg ctggccggcg ctgctgtcga ctacgccatc atggcgacgg
     1261 cgcctttcct ttgggttctc tatatcgggc ggatcgtggc cggcatcacc ggggcgactg
     1321 gggcggtagc cggcgcttat attgccgata tcactgatgg cgatgagcgc gcgcggcact
     1381 tcggcttcat gagcgcctgt ttcgggttcg ggatggtcgc gggacctgtg ctcggtgggc
     1441 tgatgggcgg tttctccccc cacgctccgt tcttcgccgc ggcagccttg aacggcctca
     1501 atttcctgac gggctgtttc cttttgccgg agtcgcacaa aggcgaacgc cggccgttac
     1561 gccgggaggc tctcaacccg ctcgcttcgt tccggtgggc ccggggcatg accgtcgtcg
     1621 ccgccctgat ggcggtcttc ttcatcatgc aacttgtcgg acaggtgccg gccgcgcttt
     1681 gggtcatttt cggcgaggat cgctttcact gggacgcgac cacgatcggc atttcgcttg
     1741 ccgcatttgg cattctgcat tcactcgccc aggcaatgat caccggccct gtagccgccc
     1801 ggctcggcga aaggcgggca ctcatgctcg gaatgattgc cgacggcaca ggctacatcc
     1861 tgcttgcctt cgcgacacgg ggatggatgg cgttcccgat catggtcctg cttgcttcgg
     1921 gtggcatcgg aatgccggcg ctgcaagcaa tgttgtccag gcaggtggat gaggaacgtc
     1981 aggggcagct gcaaggctca ctggcggcgc tcaccagcct gacctcgatc gtcggacccc
     2041 tcctcttcac ggcgatctat gcggcttcta taacaacgtg gaacgggtgg gcatggattg
     2101 caggcgctgc cctctacttg ctctgcctgc cggcgctgcg tcgcgggctt tggagaaatt
     2161 cttcaaattc ccgttgcaca tagcccggca attcctttcc ctgctctgcc ataagcgcag
     2221 cgaatgccgg gtaatactcg tcaacgatct gatagagaag ggtttgctcg ggtcggtggc
     2281 tctggtaacg accagtatcc cgatcccggc tggccgtcct ggccgccaca tgaggcatgt
     2341 tccgcgtcct tgcaatactg tgtttacata cagtctatcg cttagcggaa agttctttta
     2401 ccctcagccg aaatgcctgc cgttgctaga cattgccagc cagtgcccgt cactcccact
     2461 cttacattgt tcgcagtatc taggtacatg ttattgcgtg caccttcatg atgtataatg
     2521 ttattaatga cagcagaaag atcgacagaa ttaatattct tcgtacctgg gacagttatc
     2581 gggtgtcggc cagtgatgag accaaaggat gcagtataag atgaaccact actattatca
     2641 tggccaccat taccaccacc gttaaaacta cctcccgaac ttccagagcc gctatgacca
     2701 tcattgtagg ggtcatttgg tcccatgtga ccaaaaccac cgacagtacc gctatttaac
     2761 tctgtactca taaaattatc ctctttgact tttaaaaaca gcaaaaactg tatttataac
     2821 aatgtgttta tatacagtat tcgaaatact acgcccgtca aaaccacacc gtcaacaggt
     2881 tttttgtgta tttttcacgc ctcacgggca gcctcagacc ttttgtgtta aaacagtaat
     2941 atgttctgct gttacaatac ggtacacaca ggagacactg tcacttttat ggcatggcac
     3001 acagttcaag agacactgaa attaacagga aagtcgcgct cgcaattata tcgtgacatg
     3061 gctaaaggcc ttgtcagcta tcgcacagga aaaaatgatc gccgggagtt cgagaccagt
     3121 gaactgattc gggcctatgg cgagctgcgc acttctgaga caccaaaaag gcacaaggag
     3181 ggacactcca aatctgtcgt tagccaggcg gaaaatgctc agctagacgt cattcagcag
     3241 aaactggacg ctctgcagca gactgttacc ctaatgcttg aagacaagat ggcgcgggaa
     3301 gcagaaaacc gcctgcgaaa tgaggagagg gaacaactac aggccgaaat tagccgtctg
     3361 gctgacgcgc tggcacagga aaaaaagcgg ggattctggt cgcggctgtt tcgctccggt
     3421 tcagagtaac agtgcaggtg actctgattt accataacat ctgttatcag caccgcctga
     3481 gaggccctca gggcgtttct gacgggaaac cggtaaaaca ggcaacaaac cacccgaaaa
     3541 cccgccagaa acgcgcaggc tctgttttta cggcatgcat gagtatgcgc acaagtgcat
     3601 agcgcggaaa ttcacaccat tcagccgggg taagtcctgt ttccggcgtt cttcccatcc
     3661 gaaaacggtg aacggggacg caatttattg cgtggccgtg aagcgttgcc ggcgcagccg
     3721 gaaaacccgc tgtcagcacg ttcaggaagg gggtgatgta caaatggcac agaggatgaa
     3781 agtgacgtat gagctgaagg aatgatgtaa aagtgatgta taagtgatgt aaaagtgatg
     3841 ta
//
LOCUS       DAZPAC010000732         3655 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.guided.332, whole genome shotgun sequence.
ACCESSION   DAZPAC010000732 DAZPAC010000000
VERSION     DAZPAC010000732.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.guided.332"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..1125
                     /locus_tag="ACHUPM_005075"
     CDS             <1..1125
                     /locus_tag="ACHUPM_005075"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR0872541.1"
                     /translation="TTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGMAVA
                     VIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLSDPA
                     TKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPGTQ
                     RLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWGYRE
                     GKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYWQTG
                     DMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGATGG
                     FGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1214..1747
                     /gene="blc"
                     /locus_tag="ACHUPM_005076"
     CDS             1214..1747
                     /gene="blc"
                     /locus_tag="ACHUPM_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0872542.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1744..2061)
                     /gene="sugE"
                     /locus_tag="ACHUPM_005077"
     CDS             complement(1744..2061)
                     /gene="sugE"
                     /locus_tag="ACHUPM_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0872543.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2236..2382)
                     /gene="ecnB"
                     /locus_tag="ACHUPM_005078"
     CDS             complement(2236..2382)
                     /gene="ecnB"
                     /locus_tag="ACHUPM_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR0872544.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2493..2618)
                     /gene="ecnA"
                     /locus_tag="ACHUPM_005079"
     CDS             complement(2493..2618)
                     /gene="ecnA"
                     /locus_tag="ACHUPM_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR0872545.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2670..3236)
                     /gene="efp"
                     /locus_tag="ACHUPM_005080"
     CDS             complement(2670..3236)
                     /gene="efp"
                     /locus_tag="ACHUPM_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR0872546.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3278..>3655
                     /locus_tag="ACHUPM_005081"
     CDS             3278..>3655
                     /locus_tag="ACHUPM_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR0872547.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRY"
BASE COUNT          944 a          938 c          903 g          870 t
ORIGIN      
        1 acgacgctct gcgccttatt aattaccgcc tcttgctcca catttgctgc ccctcaacaa
       61 atcaacgata ttgtgcatcg cacaattacc ccgcttatag agcaacaaaa gatccccggt
      121 atggcggtgg cggtaattta tcagggtaaa ccttattact ttacctgggg ctatgcggac
      181 atcgccaaaa agcagcccgt cacacagcaa acgttgtttg agttaggttc ggtcagcaaa
      241 acatttacgg gcgtgcttgg tggcgacgct attgctcgag gggaaatcaa gttaagcgat
      301 cccgcaacaa aatactggcc tgaacttact gctaaacagt ggaatgggat cacactatta
      361 catcttgcga cctacaccgc tggcggcctg ccattgcagg tgccggatga agtgaaatcc
      421 tcaagcgact tgctgcgctt ctatcaaaac tggcagcctg catgggctcc aggaacacaa
      481 cgtctgtatg ccaactccag tatcggtttg ttcggcgcac tggctgtgaa gccgtctggt
      541 ttgagttttg agcaggcgat gcaaactcgt gtcttccagc cactcaaact caaccatacg
      601 tggattaatg tacctccccc agaagaaaag aattacgcct ggggatatcg cgaaggtaag
      661 gcagtgcatg tttcgccagg ggcgttagat gctgaagctt atggtgtgaa gtcgaccatt
      721 gaagatatgg cccgctgggt gcaaagcaat ttaacccccc ttgatatcaa tgagaaaacg
      781 cttcaacaag ggatacaact ggcacaatct cgctactggc aaaccggcga tatgtatcag
      841 ggcctgggct gggaaatgct ggactggccg gtcaatcctg acagcatcat taacggcagt
      901 gacaataaaa ttgcactggc agcacgcccg gtaaaagcga ttaagccccc aactcctgca
      961 gtacgcgcat catgggtaca taaaacgggg gcgaccggcg gatttggtag ctatgtcgcg
     1021 tttattccag aaaaagagct gggtatcgtg atgctggcta acaaaaacta tcccaatcca
     1081 gcgagagtcg ccgccgcctg tcagattctc aacgctctac agtaaaattt catcgggtcc
     1141 gaattttcgg accttttctg cgcttttcct tgctgtcatc tacacttaga aaaaaaccag
     1201 taaggaaaca tttatgcgcc tgctccctct cgttgccgca gcgacagctg catttctggt
     1261 cgttgcctgc agttctccta cgccgccgcg tggcgtgaca gtagtgaata atttcgacgc
     1321 taaacgctat ctcggtacct ggtatgagat tgcccgtttt gatcaccgtt ttgaacgtgg
     1381 actggaaaaa gtcaccgcaa catacagtct gcgtgatgac ggcggcctga atgtcatcaa
     1441 taaaggctat aaccctgaca gagaaatgtg gcagcagagt gaagggaaag cgtactttac
     1501 cggcgcacca acacgcgctg cgctgaaagt gtcattcttt ggtcctttct atggcggtta
     1561 taacgtcatt gcacttgatc gggaataccg ccatgcgctg gtttgcggcc cggaccgcga
     1621 ctacctgtgg atactctccc gcacgccaac catttctgac gaagtgaaac aggagatgct
     1681 ggcagtcgcg acccgggaag ggtttgatgt cagtaagttt atttgggtac agcagcccgg
     1741 tagttagtga gtgctgagtt tcagaccaat aatccccaat acgattaacg ccagactcgc
     1801 caggcgcatc gggttagcgg actcaccgag cagcacaatg ccggtgatgg ccgcgccgac
     1861 tgcgccaata cccgtccaca cggcataagc cgtccctacc ggtaacgatt tcatcgccca
     1921 ggcaagtagc gccatactga ctatcatcgc cgtcacagta ataacacttg gcgtcaaacg
     1981 actaaagccg tgggtatatt tcaggccaac ggcccatacc acttccagca gaccagcaat
     2041 aactaagata atccaggaca tatcaggctc cggagaattg gggccatccc cggtgaaaag
     2101 aggcgtttgc aggtcgtcct gcaaagctga tggttgaacg gatattttgc caggtggcag
     2161 gggaatttca actattacct ttgtgactat cgcaaaaaaa caaacggcac gacataactg
     2221 tcgtaccgtt attgcttatt gctgcgcttt cgttgctgca ccagaaatcg cgttaccgcc
     2281 atcagaaatg tcttcaccaa cgccacgcgt ggtgttgcag gcagttaata ctgttgaaag
     2341 caccagaaca gaaaagatcg ctgcaattgt cttcttcacc ataacgtctt ccttttaggt
     2401 aatgttattt atgtttgcct atagcaaatt aataatagac aacatcgcca aaaatgatgg
     2461 aaatctgatg atttttcgga agagaagaaa aattagctgg cagcgcggga gatggagttg
     2521 ccgagatgtt tgatgtcttc gccgaaaccg cgagcggtgt tacagcccgt gagcagcgtg
     2581 ctggcaagca aaacaagaac gataaggcgt ttcatcatct ctgacctgaa taagtgatgg
     2641 tgcagcctgc aggccgcacc acaaccgcat tacttcacgc gagagacgta ttcaccagag
     2701 cgggtatcca ctttgatgac ttcgccgatt tgtacgaaca gcggaacttt aaccacagcg
     2761 ccagtagaca gggtagccgg tttgccgcca gtacctgcgg tatcaccttt caggcccgga
     2821 tcggtatcaa cgatttccag ttcaacgaag ttcggcggag taacggagat cggctgacca
     2881 ttccacagag ttacgataca ctctgcctga tccagcagcc atttagcgtt gtcgccaatt
     2941 gcttttgcat cagcagacag ctgctcgaaa gtttcgttgt tcatgaagtg ccagaactca
     3001 ccgtcgttgt acaggtaagt caggttcata tcgacaacat cagcgccttc agcggaatca
     3061 gtagatttga aggttttttc tacgcgagta ccggtcagca gacgacgcag tttaacgcga
     3121 gcaaatgcct ggcctttacc cggttttacg aattcactcg cttcaaccgc gtaaggttcg
     3181 ccgtctaaca tgattttaag accagcacga aaatcgttgc tatagtacgt tgccataagg
     3241 ccctctgaaa tttgttaatt ggtagctaag ccacaaaatg gcgcatattg taaccctaaa
     3301 taccccatcc agagaagatt ggttaacgca acttgccgat gttgtgaccg atcctggtga
     3361 acttctgcgt cttttgaata tagacgcgga cgaaaaactg ttggccggac gcagcgccaa
     3421 aaagctgttt gccctgcgtg tgccccgctc atttatcgat cgcatggaga aaggcaatcc
     3481 ggacgatcct attttgcgtc aggtacttac ctcgcaagat gagtttgtcg tcgcgtccgg
     3541 attctccacc gacccactgg aagaacagca cagcgtagtg cctggtttgt tgcataaata
     3601 ccacaaccgg gcgcttttgc tggtcaaagg cggctgcgcg gtaaattgcc gctat
//
LOCUS       DAZPAC010000733         3568 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.guided.335, whole genome shotgun sequence.
ACCESSION   DAZPAC010000733 DAZPAC010000000
VERSION     DAZPAC010000733.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.guided.335"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            99..257
                     /locus_tag="ACHUPM_005082"
     CDS             99..257
                     /locus_tag="ACHUPM_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0872548.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            717..1532
                     /gene="sul2"
                     /locus_tag="ACHUPM_005083"
     CDS             717..1532
                     /gene="sul2"
                     /locus_tag="ACHUPM_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR0872549.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1593..2396
                     /locus_tag="ACHUPM_005084"
                     /note="aph(3'')-Ib"
     CDS             1593..2396
                     /locus_tag="ACHUPM_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFR0872550.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2396..3232
                     /locus_tag="ACHUPM_005085"
     CDS             2396..3232
                     /locus_tag="ACHUPM_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR0872551.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          768 a          990 c          985 g          825 t
ORIGIN      
        1 ttcggtttac cggtgtcatt ccgctgttat ggccgcggtt tctcattcca cgcctgacac
       61 tcagttccgt gaaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
      121 ccgaccgctg cgccttgtcc ggtaactatc aacttgagtc caacccggaa agacacgaca
      181 aaacgccact ggcagcagcc attagtaaca ggagtgcacg agaagataga ttaagaggtc
      241 ttgaagtaga ggcctgactg cggctacact gaaagaacag ttttggtaat ggcggtctcg
      301 tgaaccctgt taccgggtta agcagttccc caactgactt aaccttcgaa caaaccgcct
      361 ccccaggcgg ttttttcgtt tacagagcaa gagattacga ttcattgaaa aggatctcaa
      421 gaagatcctt tgtagattac tatcaattaa tcttccgaac atgacaccag tcactgttga
      481 ttacggttaa accgctccgt aacaggtttc cctcgtattg gtgacttgca gcaccaaaga
      541 agcaaaaaag ataaatatat ctacttattg agagatgata tatttatttc caaaatgcta
      601 cagatacatc atcatgaaac ggatcaccgc aaacaggtta ctcgcctggg gattcccttt
      661 cgacccgagc atccgtatga gactcatgct cgattattat tattatagaa gcccccatga
      721 ataaatcgct catcattttc ggcatcgtca acataacctc ggacagtttc tccgatggag
      781 gccggtatct ggcgccagac gcagccattg cgcaggcgcg taagctgatg gccgaggggg
      841 cagatgtgat cgacctcggt ccggcatcca gcaatcccga cgccgcgcct gtttcgtccg
      901 acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct caaggcagat ggcattcccg
      961 tctcgctcga cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt ggtgtggcct
     1021 atctcaatga tattcgcggt tttccagacg ctgcgttcta tccgcaattg gcgaaatcat
     1081 ctgccaaact cgtcgttatg cattcggtgc aagacgggca ggcagatcgg cgcgaggcac
     1141 ccgctggcga catcatggat cacattgcgg cgttctttga cgcgcgcatc gcggcgctga
     1201 cgggtgccgg tatcaaacgc aaccgccttg tccttgatcc cggcatgggg ttttttctgg
     1261 gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt cgatgaattg cggctgcgct
     1321 tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt tctgcgcgcg ctcacaggcc
     1381 gtggtccggg ggatgtcggg gccgcgacac tcgctgcaga gcttgccgcc gccgcaggtg
     1441 gagctgactt catccgcaca cacgagccgc gccccttgcg cgacgggctg gcggtattgg
     1501 cggcgctgaa agaaaccgca agaattcgtt aactgcacat tcgggatatt tctctatatt
     1561 cgcgcttcat cagaaaactg aaggaacctc cattgaatcg aactaatatt ttttttggtg
     1621 aatcgcattc tgactggttg cctgtcagag gcggagaatc tggtgatttt gtttttcgac
     1681 gtggtgacgg gcatgccttc gcgaaaatcg cacctgcttc ccgccgcggt gagctcgctg
     1741 gagagcgtga ccgcctcatt tggctcaaag gtcgaggtgt ggcttgcccc gaggtcatca
     1801 actggcagga ggaacaggag ggtgcatgct tggtgataac ggcaattccg ggagtaccgg
     1861 cggctgatct gtctggagcg gatttgctca aagcgtggcc gtcaatgggg cagcaacttg
     1921 gcgctgttca cagcctatcg gttgatcaat gtcagtttga gcgcaggctg tcgcgaatgt
     1981 tcggacgcgc cgttgatgtg gtgtcccgca atgccgtcaa tcccgacttc ttaccggacg
     2041 aggacaagag tacgccgcag ctcgatcttt tggctcgtgt cgaacgagag ctaccggtgc
     2101 ggctcgacca agagcgcacc gatatggttg tttgccatgg tgatccctgc atgccgaact
     2161 tcatggtgga ccctaaaact cttcaatgca cgggtctgat cgaccttggg cggctcggaa
     2221 cagcagatcg ctatgccgat ttggcactca tgattgctaa cgccgaagag aactgggcag
     2281 cgccagatga agcagagcgc gccttcgctg tcctattcaa tgtattgggg atcgaagccc
     2341 ccgaccgcga acgccttgcc ttctatctgc gattggaccc tctgacttgg ggttgatgtt
     2401 catgccgcct gtttttcctg ctcattggca cgtttcgcaa cctgttctca ttgcggacac
     2461 cttttccagc ctcgtttgga aagtttcatt gccagacggg actcctgcaa tcgtcaaggg
     2521 attgaaacct atagaagaca ttgctgatga actgcgcggg gccgactatc tggtatggcg
     2581 caatgggagg ggagcagtcc ggttgctcgg tcgtgagaac aatctgatgt tgctcgaata
     2641 tgccggggag cgaatgctct ctcacatcgt tgccgagcac ggcgactacc aggcgaccga
     2701 aattgcagcg gaactaatgg cgaagctgta tgccgcatct gaggaacccc tgccttctgc
     2761 ccttctcccg atccgggatc gctttgcagc tttgtttcag cgggcgcgcg atgatcaaaa
     2821 cgcaggttgt caaactgact acgtccacgc ggcgattata gccgatcaaa tgatgagcaa
     2881 tgcctcggaa ctgcgtgggc tacatggcga tctgcatcat gaaaacatca tgttctccag
     2941 tcgcggctgg ctggtgatag atcccgtcgg tctggtcggt gaagtgggct ttggcgccgc
     3001 caatatgttc tacgatccgg ctgacagaga cgacctttgt ctcgatccta gacgcattgc
     3061 acagatggcg gacgcattct ctcgtgcgct ggacgtcgat ccgcgtcgcc tgctcgacca
     3121 ggcgtacgct tatgggtgcc tttccgcagc ttggaacgcg gatggagaag aggagcaacg
     3181 cgatctagct atcgcggccg cgatcaagca ggtgcgacag acgtcatact agatatcaag
     3241 cgacttctcc tatcccctgg gaacacatca atctcaccgg agaatatcgc tggccaaagc
     3301 cttagcgtag gattccgccc cttcccgcaa acgacccctg gaagccccgt agcgacgcgg
     3361 agaggggcga gacaagccaa gggcgcaggc tcgatgcgca gcacgacata gccggttctc
     3421 gcaaggacga gaatttccct gcggtgcccc tcaagtgtca atgaaagttt ccaacgcgag
     3481 ccattcgcga gagccttgag tccacgctag atctatctca tctgcgcaag gcagaacgtg
     3541 aagacggccg ccctggacct cgcccgcg
//
LOCUS       DAZPAC010000734         3242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.guided.352, whole genome shotgun sequence.
ACCESSION   DAZPAC010000734 DAZPAC010000000
VERSION     DAZPAC010000734.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.guided.352"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            8..940
                     /locus_tag="ACHUPM_005086"
     CDS             8..940
                     /locus_tag="ACHUPM_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR0872552.1"
                     /translation="MPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWAD
                     ENGDLGPVYGKQWRRWEDTRIVSHSEYLSKIATFRERGYKVEGYLGISEDRVVLSREI
                     DQLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMAT
                     DIGLQHSRFGYESIYTKMLYDLEMDGSVTETELDELGIPKRILNSCLVQRSVDTFVGM
                     PFNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMKRQAPEHSDPVV
                     IFPHEWSELDDFKWDDILILGYDPLPWIKVPVAV"
     gene            942..1355
                     /locus_tag="ACHUPM_005087"
     CDS             942..1355
                     /locus_tag="ACHUPM_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872553.1"
                     /translation="MARGMYVLCEIEGVLANTSHRKSVPDTDAGQLIAGDELIFPTSR
                     MLRGFARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNGTSYEKHIKRTLA
                     EHKGDLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            1352..1828
                     /locus_tag="ACHUPM_005088"
     CDS             1352..1828
                     /locus_tag="ACHUPM_005088"
                     /inference="COORDINATES: protein motif:HMM:NF047324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFR0872554.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETVFTQRASEEEPGFSQIVYRRS"
     gene            1828..2472
                     /locus_tag="ACHUPM_005089"
     CDS             1828..2472
                     /locus_tag="ACHUPM_005089"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0872555.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGESMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            2534..2953
                     /locus_tag="ACHUPM_005090"
     CDS             2534..2953
                     /locus_tag="ACHUPM_005090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872556.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGAQVGEAINIAMSQFSEDSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TASGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            2963..>3242
                     /locus_tag="ACHUPM_005091"
     CDS             2963..>3242
                     /locus_tag="ACHUPM_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFR0872557.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMEACSAWVAH"
BASE COUNT          828 a          709 c          838 g          867 t
ORIGIN      
        1 tgggactgtg ccgcttattt ctggaaaggc ggtaaatctt aagccactgc ttgtcgaact
       61 tgagtggtat ctgaaaggca cgggcaacat ccaatttctt aaggataacg gcgttaagat
      121 ttgggatgca tgggccgatg agaatggcga tttggggccg gtttacggta agcagtggcg
      181 tcgatgggaa gatacccgca tcgtgagcca tagtgaatat ctgagcaaga tcgctacttt
      241 ccgtgaacgc gggtacaaag tcgagggata cctgggtatc agtgaagatc gcgtagtgct
      301 gtcccgtgaa atcgatcagc tacagcgtat tgtcgacaca ctgcgcacga accctaccga
      361 tcgtcgcatc atgcttaacg catggaacgt aggcgagctt gaggatatga aactgccacc
      421 ttgccacttt gtcttctctt tgtggagtcg tgagcttgat tttgaaaccc gtttaacgat
      481 ggcaactgac attggtcttc aacacagtcg cttcggttat gagtctatct acaccaagat
      541 gctatacgat ctggagatgg acggcagtgt tactgaaact gaactggatg aacttggaat
      601 ccccaaacgc atcctcaact cctgcctcgt acagcgtagc gtagacactt ttgttggtat
      661 gccattcaat attgctggct atggcattct cactcatttt ctcgcgaaga ttacgggtca
      721 catggccggt gcatttgtgc attttggctt tgacgtgcat ttgtacaaca accacatgga
      781 aggtgtgtgt gagctaatga aacgacaggc tccagagcat tcagatccgg tcgttatttt
      841 ccctcatgaa tggtctgagc tggatgattt caaatgggac gatattctga ttcttggcta
      901 tgaccctcta ccgtggatca aggttccagt ggcggtgtga tatggcaaga ggtatgtatg
      961 tcttatgcga aattgaaggt gtgctggcaa ataccagcca tcgtaaatct gtacctgaca
     1021 cggatgcagg ccagctcatt gccggtgatg aactcatttt ccccaccagc cgtatgttgc
     1081 gtggttttgc tcgctcaggg gctgaagtgg tgcttatcag tagccgctct gaaactcttg
     1141 aagcgcccac taaacgatgg ctgaaagatt ttggcgttga ttacgactgg cttcatctcg
     1201 taccgaatgg caccagttat gagaagcata ttaagcgcac attagcggag cataaaggcg
     1261 atctgcttat cgctgcgctg gtgcacgatc ctcgactccg tgccgcttta gctgactctc
     1321 atcatagacc ggtcatctat gaggtgagca aatgaagatg atagctgctg ttggccgtaa
     1381 ctatgagatc ggcatagcga acgaactgcc ctggcgttgt tctaccgatc tgaagctatt
     1441 taagagactc accaaaaacg ccactgtcgt tatggggcgt aaaacgatgg aaagtctcaa
     1501 acgccctctt ccagagcgtc ataacctcgt tttgacgcgc tctcatggct ttgtaccaaa
     1561 tggattctac cctgctggtg tggatgatgt gttgcgatta ccagagcctg tgtgggtgat
     1621 tggcggggaa caaatttact cgctattcat gccgcatgtt gaagagattt ggctctccca
     1681 catcggcgtt gacgtgccaa acgccgatgc attcttcccg gcaagcatga tgcgtaattt
     1741 aggctttgtg cctgttgaaa cagtttttac ccaacgagcc agcgaggaag agcctggctt
     1801 ttcgcagatc gtatacagaa ggtcgtaatg gattaccgga ttgggatcac tggtgctcag
     1861 ggcagtggga aaacaaccct ggcaaaatat atcgacaaac attacggaat cccttacgtg
     1921 gatgctggtg tcggaagttt gatgagccga ctcggtgttc gagtaggtga gtctatgcct
     1981 ctatatgagc ggcttcagat tcaaatggaa atcgcaaagc atatagagct acttacgcgt
     2041 ggtgttgaag gctttgttat cgatcgcaca cctgctgatg ttatggccta cacgttggat
     2101 ttggtcggcc ataccaatga agatcgatgt attgagttag ccctcgatat cgaaaagttt
     2161 tgccacaaaa ctgctatttc aaactttaac gccattgctg gcctacgccc gggagtcgct
     2221 ctctcagagc gagattactt gcgatcacaa cgagcatcat tagaccgtct gtatgtcgct
     2281 cgtattgatg cgttgatgtg cggggaactg acaaaaattc acctgcatcc gcaaaggggg
     2341 gatctgcaaa ccttcgtcgt ttccaaccga tatcgcacgg ttgaagcaag agccagatca
     2401 gtgatgagaa tgctagataa cgctgtagaa aagatagaaa accggttctg tggtcgagtg
     2461 accgttcatt agaaattgtt cgcctcttcg acattgcgac aataaaactc tcaaaatggg
     2521 ttaaggataa aaaatgttta gtgaaatgtt gcttgaagat gaactggatc ggaaaacaac
     2581 agaggctttg attcgtgtag cggacgaaca ttcccggtcg cttatgagcg atcgagaggc
     2641 tcgtctggct attcgtgcca tattcgaaac tgcgcagggg cttgttggcg cacaagtagg
     2701 tgaagccatt aacatcgcca tgtctcagtt cagtgaagac agtaaaaagc ctctgtttcc
     2761 tatgcatttg atgctggctg gtggcacggt gctttatatc tctgtttgtc tggatagcaa
     2821 ccaaatcaat attctcaaca ctgcgtcagg taagtggaaa gatccgattg tctgtgaaac
     2881 cagtgaagaa actttgaaaa aagcggctca atttgtacgt agcgcactac ttaagggcgc
     2941 taagaagttg taaggagttc tgatgacaac gattgttgca ggcatcgata tcgagtctac
     3001 gggactggat ttccttgctg gtcataaaat tattgaaatc gcaattaccc gctatgaact
     3061 ggagacacag agacatattg atagtctgga gatgcgtttt aaccctcgca gaaacataga
     3121 tccgaaagct caagccgttc atggcatttc attagaacag ctcgcagctg aacctttgtt
     3181 gtcaaatcat gctagcgaaa ttggcgctta tatggaggca tgtagtgcgt gggttgctca
     3241 ta
//
LOCUS       DAZPAC010000735         3242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.guided.353, whole genome shotgun sequence.
ACCESSION   DAZPAC010000735 DAZPAC010000000
VERSION     DAZPAC010000735.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.guided.353"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            8..940
                     /locus_tag="ACHUPM_005092"
     CDS             8..940
                     /locus_tag="ACHUPM_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR0872558.1"
                     /translation="MPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWAD
                     ENGDLGPVYGKQWRRWEDTRIVSHSEYLSKIATFRERGYKVEGYLGISEDRVVLSREI
                     DQLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMAT
                     DIGLQHSRFGYESIYTKMLYDLEMDGSVTETELDELGIPKRILNSCLVQRSVDTFVGM
                     PFNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMKRQAPEHSDPVV
                     IFPHEWSELDDFKWDDILILGYDPLPWIKVPVAV"
     gene            942..1355
                     /locus_tag="ACHUPM_005093"
     CDS             942..1355
                     /locus_tag="ACHUPM_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872559.1"
                     /translation="MARGMYVLCEIEGVLANTSHRKSVPDTDAGQLIAGDELIFPTSR
                     MLRGFARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNGTSYEKHIKRTLA
                     EHKGDLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            1352..1828
                     /locus_tag="ACHUPM_005094"
     CDS             1352..1828
                     /locus_tag="ACHUPM_005094"
                     /inference="COORDINATES: protein motif:HMM:NF047324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFR0872560.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETVFTQRASEEEPGFSQIVYRRS"
     gene            1828..2472
                     /locus_tag="ACHUPM_005095"
     CDS             1828..2472
                     /locus_tag="ACHUPM_005095"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0872561.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGESMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            2534..2953
                     /locus_tag="ACHUPM_005096"
     CDS             2534..2953
                     /locus_tag="ACHUPM_005096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872562.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGAQVGEAINIAMSQFSEDSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TASGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            2963..>3242
                     /locus_tag="ACHUPM_005097"
     CDS             2963..>3242
                     /locus_tag="ACHUPM_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFR0872563.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMEACSAWVAH"
BASE COUNT          828 a          708 c          838 g          868 t
ORIGIN      
        1 tgggactgtg ccgcttattt ctggaaaggc ggtaaatctt aagccactgc ttgtcgaact
       61 tgagtggtat ctgaaaggca cgggcaacat ccaatttctt aaggataacg gcgttaagat
      121 ttgggatgca tgggccgatg agaatggcga tttggggccg gtttacggta agcagtggcg
      181 tcgatgggaa gatacccgca tcgtgagcca tagtgaatat ctgagcaaga tcgctacttt
      241 ccgtgaacgc gggtacaaag tcgagggata cctgggtatc agtgaagatc gcgtagtgct
      301 gtcccgtgaa atcgatcagc tacagcgtat tgtcgacaca ctgcgcacga accctaccga
      361 tcgtcgcatc atgcttaacg catggaacgt aggcgagctt gaggatatga aactgccacc
      421 ttgccacttt gtcttctctt tgtggagtcg tgagcttgat tttgaaaccc gtttaacgat
      481 ggcaactgac attggtcttc aacacagtcg cttcggttat gagtctatct acaccaagat
      541 gctatacgat ctggagatgg acggcagtgt tactgaaact gaactggatg aacttggaat
      601 ccccaaacgc atcctcaact cctgcctcgt acagcgtagc gtagacactt ttgttggtat
      661 gccattcaat attgctggct atggcattct cactcatttt ctcgcgaaga ttacgggtca
      721 catggccggt gcatttgtgc attttggctt tgacgtgcat ttgtacaaca accacatgga
      781 aggtgtgtgt gagctaatga aacgacaggc tccagagcat tcagatccgg tcgttatttt
      841 ccctcatgaa tggtctgagc tggatgattt caaatgggac gatattctga ttcttggcta
      901 tgaccctcta ccgtggatca aggttccagt ggcggtgtga tatggcaaga ggtatgtatg
      961 tcttatgcga aattgaaggt gtgctggcaa ataccagcca tcgtaaatct gtacctgaca
     1021 cggatgcagg ccagctcatt gccggtgatg aactcatttt ccccaccagc cgtatgttgc
     1081 gtggttttgc tcgctcaggg gctgaagtgg tgcttatcag tagccgctct gaaactcttg
     1141 aagcgcccac taaacgatgg ctgaaagatt ttggcgttga ttacgactgg cttcatctcg
     1201 taccgaatgg caccagttat gagaagcata ttaagcgcac attagcggag cataaaggcg
     1261 atctgcttat cgctgcgctg gtgcacgatc ctcgactccg tgccgcttta gctgactctc
     1321 atcatagacc ggtcatctat gaggtgagca aatgaagatg atagctgctg ttggccgtaa
     1381 ctatgagatc ggcatagcga acgaactgcc ctggcgttgt tctaccgatc tgaagctatt
     1441 taagagactc accaaaaacg ccactgtcgt tatggggcgt aaaacgatgg aaagtctcaa
     1501 acgccctctt ccagagcgtc ataacctcgt tttgacgcgc tctcatggct ttgtaccaaa
     1561 tggattctac cctgctggtg tggatgatgt gttgcgatta ccagagcctg tgtgggtgat
     1621 tggcggggaa caaatttact cgctattcat gccgcatgtt gaagagattt ggctctccca
     1681 catcggcgtt gatgtgccaa acgccgatgc attcttcccg gcaagcatga tgcgtaattt
     1741 aggctttgtg cctgttgaaa cagtttttac ccaacgagcc agcgaggaag agcctggctt
     1801 ttcgcagatc gtatacagaa ggtcgtaatg gattaccgga ttgggatcac tggtgctcag
     1861 ggcagtggga aaacaaccct ggcaaaatat atcgacaaac attacggaat cccttacgtg
     1921 gatgctggtg tcggaagttt gatgagccga ctcggtgttc gagtaggtga gtctatgcct
     1981 ctatatgagc ggcttcagat tcaaatggaa atcgcaaagc atatagagct acttacgcgt
     2041 ggtgttgaag gctttgttat cgatcgcaca cctgctgatg ttatggccta cacgttggat
     2101 ttggtcggcc ataccaatga agatcgatgt attgagttag ccctcgatat cgaaaagttt
     2161 tgccacaaaa ctgctatttc aaactttaac gccattgctg gcctacgccc gggagtcgct
     2221 ctctcagagc gagattactt gcgatcacaa cgagcatcat tagaccgtct gtatgtcgct
     2281 cgtattgatg cgttgatgtg cggggaactg acaaaaattc acctgcatcc gcaaaggggg
     2341 gatctgcaaa ccttcgtcgt ttccaaccga tatcgcacgg ttgaagcaag agccagatca
     2401 gtgatgagaa tgctagataa cgctgtagaa aagatagaaa accggttctg tggtcgagtg
     2461 accgttcatt agaaattgtt cgcctcttcg acattgcgac aataaaactc tcaaaatggg
     2521 ttaaggataa aaaatgttta gtgaaatgtt gcttgaagat gaactggatc ggaaaacaac
     2581 agaggctttg attcgtgtag cggacgaaca ttcccggtcg cttatgagcg atcgagaggc
     2641 tcgtctggct attcgtgcca tattcgaaac tgcgcagggg cttgttggcg cacaagtagg
     2701 tgaagccatt aacatcgcca tgtctcagtt cagtgaagac agtaaaaagc ctctgtttcc
     2761 tatgcatttg atgctggctg gtggcacggt gctttatatc tctgtttgtc tggatagcaa
     2821 ccaaatcaat attctcaaca ctgcgtcagg taagtggaaa gatccgattg tctgtgaaac
     2881 cagtgaagaa actttgaaaa aagcggctca atttgtacgt agcgcactac ttaagggcgc
     2941 taagaagttg taaggagttc tgatgacaac gattgttgca ggcatcgata tcgagtctac
     3001 gggactggat ttccttgctg gtcataaaat tattgaaatc gcaattaccc gctatgaact
     3061 ggagacacag agacatattg atagtctgga gatgcgtttt aaccctcgca gaaacataga
     3121 tccgaaagct caagccgttc atggcatttc attagaacag ctcgcagctg aacctttgtt
     3181 gtcaaatcat gctagcgaaa ttggcgctta tatggaggca tgtagtgcgt gggttgctca
     3241 ta
//
LOCUS       DAZPAC010000736         2426 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.guided.397, whole genome shotgun sequence.
ACCESSION   DAZPAC010000736 DAZPAC010000000
VERSION     DAZPAC010000736.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.guided.397"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            116..904
                     /locus_tag="ACHUPM_005098"
     CDS             116..904
                     /locus_tag="ACHUPM_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFR0872564.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            1110..1457
                     /locus_tag="ACHUPM_005099"
     CDS             1110..1457
                     /locus_tag="ACHUPM_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR0872565.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            1451..2290
                     /gene="sul1"
                     /locus_tag="ACHUPM_005100"
     CDS             1451..2290
                     /gene="sul1"
                     /locus_tag="ACHUPM_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR0872566.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
BASE COUNT          473 a          699 c          697 g          557 t
ORIGIN      
        1 ccagtcgctt cgctccttgg acagctttta agtcgcgtct ttgtggtttt gctgcgcaaa
       61 agtattccac aaagccgcaa cttaaaagct gccgctgaac ttaacgttag gcatcatggg
      121 tgaatttttc cctgcacaag ttttcaagca gctgtcccac gctcgcgcgg tgatcgagcg
      181 ccatctggct gcgacactgg acacaatcca cctgttcgga tctgcgatcg atggagggct
      241 gaagccggac agcgacatag acttgctcgt gaccgtcagc gccgcaccta acgattcgct
      301 ccggcaggcg ctaatgctcg atttgctgaa agtctcatca ccgccaggcg atggcggaac
      361 atggcgaccg ctggagctaa ctgttgtcgc tcgaagcgaa gtagtgcctt ggcgctatcc
      421 ggcgcggcgt gagcttcagt tcggtgagtg gctccgccac gacatccttt ccggaacgtt
      481 cgagcctgcc gttctggatc acgatcttgc gattttgctg accaaggcga ggcaacacag
      541 ccttgcgctt ctaggcccat ccgcagccac gtttttcgag ccggtgccga aggagcattt
      601 ctccaaggcg cttttcgaca ctattgccca gtggaatgca gagtcggatt ggaagggtga
      661 cgagcggaac gtcgttcttg ctcttgctcg catttggtac agcgcttcaa ctggtctcat
      721 tgctcctaag gacgttgctg ccgcatgggt atcggagcgt ttgcctgccg agcatcggcc
      781 cctcatctgc aaggcacgcg cggcgtacct gggtagcgag gacgacgacc tagcaatgcg
      841 cgtcgaagag acggccgcgt tcgttcgata tgccaaagca acgattgaga gaatcttgcg
      901 ttgagcggca tgtgcgaaaa gtgcatcgac ccgcgccgag ggcatctgat gcctaactcg
      961 gcgttcaagc ggacgggctg cgcccgccgc tcaactatgc gttagatgca ctaagcacat
     1021 aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa
     1081 taagccctac acaaattggg agatatatca tgaaaggctg gctttttctt gttatcgcaa
     1141 tagttggcga agtaatcgca acatccgcat taaaatctag cgagggcttt actaagcttg
     1201 ccccttccgc cgttgtcata atcggttatg gcatcgcatt ttattttctt tctctggttc
     1261 tgaaatccat ccctgtcggt gttgcttatg cagtctggtc gggactcggc gtcgtcataa
     1321 ttacagccat tgcctggttg cttcatgggc aaaagcttga tgcgtggggc tttgtaggta
     1381 tggggctcat aattgctgcc tttttgctcg cccgatcccc atcgtggaag tcgctgcgga
     1441 ggccgacgcc atggtgacgg tgttcggcat tctgaatctc accgaggact ccttcttcga
     1501 tgagagccgg cggctagacc ccgccggcgc tgtcaccgcg gcgatcgaaa tgctgcgagt
     1561 cggatcagac gtcgtggatg tcggaccggc cgccagccat ccggacgcga ggcctgtatc
     1621 gccggccgat gagatcagac gtattgcgcc gctcttagac gccctgtccg atcagatgca
     1681 ccgtgtttca atcgacagct tccaaccgga aacccagcgc tatgcgctca agcgcggcgt
     1741 gggctacctg aacgatatcc aaggatttcc tgaccctgcg ctctatcccg atattgctga
     1801 ggcggactgc aggctggtgg ttatgcactc agcgcagcgg gatggcatcg ccacccgcac
     1861 cggtcacctt cgacccgaag acgcgctcga cgagattgtg cggttcttcg aggcgcgggt
     1921 ttccgccttg cgacggagcg gggtcgctgc cgaccggctc atcctcgatc cggggatggg
     1981 atttttcttg agccccgcac cggaaacatc gctgcacgtg ctgtcgaacc ttcaaaagct
     2041 gaagtcggcg ttggggcttc cgctattggt ctcggtgtcg cggaaatcct tcttgggcgc
     2101 caccgttggc cttcctgtaa aggatctggg tccagcgagc cttgcggcgg aacttcacgc
     2161 gatcggcaat ggcgctgact acgtccgcac ccacgcgcct ggagatctgc gaagcgcaat
     2221 caccttctcg gaaaccctcg cgaaatttcg cagtcgcgac gccagagacc gagggttaga
     2281 tcatgcctag cattcacctt ccggccgccc gctagcggac cctggtcagg ttccgcgaag
     2341 gtgggcgcag acatgctggg ctcgtcagga tcaaactgca ctatgaggcg gcggttcata
     2401 ccgcgccagg ggagcgaatg gacagc
//
LOCUS       DAZPAC010000737         2137 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.guided.409, whole genome shotgun sequence.
ACCESSION   DAZPAC010000737 DAZPAC010000000
VERSION     DAZPAC010000737.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.guided.409"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..290
                     /locus_tag="ACHUPM_005101"
     CDS             <1..290
                     /locus_tag="ACHUPM_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0872567.1"
                     /translation="RKTALQGGFFVYRARDYDATERISRRSFTVMEIYPIRAHRIHIV
                     ITLDLREFQQQQEKDFLQTSLQQAKFNQKKAAELLGLTYHQLRALLKKHQI"
     gene            384..1028
                     /locus_tag="ACHUPM_005102"
     CDS             384..1028
                     /locus_tag="ACHUPM_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="HFR0872568.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQ
                     FYDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFM
                     NMITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGE
                     FSTFDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
     gene            complement(1410..1646)
                     /locus_tag="ACHUPM_005103"
     CDS             complement(1410..1646)
                     /locus_tag="ACHUPM_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0872569.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARA
                     QIELQRYADRQRALREDERQQSDFAWSRPRAPRR"
BASE COUNT          552 a          520 c          551 g          514 t
ORIGIN      
        1 ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag agcaagagat tacgacgcca
       61 cagaaaggat ctcaagaaga tccttcaccg taatggaaat ctatccaatc cgagcacata
      121 ggatacacat cgttataacg ctggatctgc gtgaattcca gcagcagcaa gagaaagact
      181 ttctacaaac cagcttacag caggctaaat ttaaccagaa aaaagccgct gaattactgg
      241 gtctgactta tcatcagcta cgtgccttgc tgaagaaaca ccagatctaa tacgcatttg
      301 caggtgtcat actggcgggt ttgacgcata acgtcataag gtttaccatg gcgtcattac
      361 tgtataaaaa cacaggcata gatatgactc tggcattagt tggcgaaaaa attgacagaa
      421 atcgcttcac cggtgagaaa gttgaaaata gtacattttt taactgcgat ttttcaggtg
      481 ccgacctgag cggcactgaa tttatcggct gccagttcta tgatcgcgaa agtcagaaag
      541 ggtgcaattt tagtcgcgca atgctgaaag atgccatttt caaaagctgt gatttatcaa
      601 tggcagattt ccgcaacgtc agtgccttgg gcattgaaat tcgccactgc cgcgcacaag
      661 gcgcagattt ccgcggtgca agctttatga atatgatcac cacgcgcacc tggttttgca
      721 gcgcatatat cactaatact aatctaagct acgccaattt ttcgaaagtc gtgttggaaa
      781 agtgtgagct gtgggaaaac cgctggatgg ggactcaggt actgggtgcg acgttcagtg
      841 gttcagatct ctccggcggc gagttttcga ctttcgactg gcgagcagca aacttcacac
      901 attgcgatct gaccaattcg gagttaggtg acttagatat tcggggtgtt gatttacaag
      961 gcgttaagtt agacagctac caggcatcgt tgctcatgga gcggcttggc atcgctgtga
     1021 ttggttagtt cagcgtttac accgggagat gcgtttgacg atttactttt acccgcgatc
     1081 atcgcgttta ttgagtttat tatttatcta tgcgattccg atgaaaaatt cgcacgaaaa
     1141 tatggctaac tcatagcaaa aaaaaagccc cctaagggct tttttttgta cctgacggtc
     1201 agaccgatta aacataacat cccttgcccg cacccccgca ggcgccctca gcgcaatcct
     1261 gctgcatcag ggccgtttcc agcacctttc ctcaccagta tcccggaaaa acgcccctga
     1321 tgatttctgc gcgactgcat ggctatgcac gcaggtgaat ggcaggtgat ttcgcagcgt
     1381 cagcaccact aaaccgccaa aagccaaaat cagcggcgcg gcgcgcgcgg acgtgaccag
     1441 gcaaaatcgg actgctgccg ctcatcctca cgtaatgcac gctggcggtc ggcgtaacgc
     1501 tgcaactcaa tttgcgcacg cgctctgttc aggacgatgt gccggttagg ctgatttagc
     1561 tttttacctg acgctctttc agctgcggca acagcatcat caagaaattt tttctctgca
     1621 ggcgtcaagt ttgtcagtct ggccataacg ttctccagtc agaataaatc agcacagtct
     1681 gccagacaca gtagaaacga tgaagcgcca gcgacggtga aaggcagtca gaaacggtga
     1741 acgggagcgg attttaatcc ggtgccgtga ggcgttggac aagccgcagg cgcgtcatta
     1801 gcttttagcc ggtttcgggg cgcagccctg aaccagtcac gtagcgctag cggagtgtat
     1861 actggctgac tatgttggca cggatgtgga aatcagtgaa gtgcttcatg tagcaggaga
     1921 aaaaaggcgg caccagcgcg tcagcaggat atgtagtaca ggatatattc cgcttcctcg
     1981 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtgccggc ttacaggcgg
     2041 ggcggaaatt tcctgggaga taccaggaag gagcttaaca gggaagagat aaggccgcgg
     2101 cgaagccgtt tttccatagg ctccgccccc ctgacaa
//
LOCUS       DAZPAC010000738         1813 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.guided.435, whole genome shotgun sequence.
ACCESSION   DAZPAC010000738 DAZPAC010000000
VERSION     DAZPAC010000738.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.guided.435"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            112..207
                     /locus_tag="ACHUPM_005104"
     CDS             112..207
                     /locus_tag="ACHUPM_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFR0872570.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            333..1070
                     /gene="erm(B)"
                     /locus_tag="ACHUPM_005105"
     CDS             333..1070
                     /gene="erm(B)"
                     /locus_tag="ACHUPM_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFR0872571.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          663 a          303 c          295 g          552 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc aaattttgta taataggaat
       61 tgaagttaaa ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta
      121 ttccaaatgc gttatcaaat gcgttatgta gataaaacat ctactgtttt gaaacagact
      181 aaaaaaagtg attacgcaga taaataaata cgttagatta attcctacca gtgactaatc
      241 ttatgacttt ttaaacagat aactaaaatt acaaacaaat cgtttaactt ctgtatttgt
      301 ttatagatgt aatcacttca ggagtaaatt acatgaacaa aaatataaaa tattctcaaa
      361 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
      421 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
      481 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa
      541 aattaaaact gaatactcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
      601 ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta
      661 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat
      721 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
      781 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca agagtaaaca
      841 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
      901 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
      961 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccgtt acttatgagc
     1021 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
     1081 cgcttttgta aatttggaaa gttacacgtt actaaaggga atgtagataa attattaggt
     1141 atactactga cagcttccaa ggagctaaag aggtccctag actagcaaga agtacacaag
     1201 aagccttaaa gattatagaa aagcttttcc atgaaatgct taacattatt taatgttaac
     1261 atgtggcttt gtggtaacta ccacgagtgg taacttgtaa catgaaaaaa gttacaagtt
     1321 acaagtggtt tatttacgaa cataaaggct aggaaagcac ttgataagta tatcaagtgc
     1381 tttttttccg ttcgatttta ctatccaaga taccgcttga cgtacctaat attttagtgt
     1441 gctaagggct ttgctttttc tggttatagt tcgttcaaaa aagaccgcaa gggtaaatgc
     1501 acgatcatag atcgcccttg cagcttttta atctctccac ttttttataa ccaagcaaac
     1561 caccaagcac actgaaaaaa taattaggaa ctaaagcgaa gtgcgtatct tggataaatc
     1621 ttcactcgat tttaaaagat taagcaccca gccaaagcgc ctaaaaaaat aattttttta
     1681 aagaaaggaa gtaatttaaa tgactacaat tttaagcgac tacaataaat ctatcctaat
     1741 tgaattacta aacaccaatc gccaaattat cgtcgttggt gatgctgcca acttactgat
     1801 ttagtgtatg atg
//
LOCUS       DAZPAC010000739         1704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.guided.444, whole genome shotgun sequence.
ACCESSION   DAZPAC010000739 DAZPAC010000000
VERSION     DAZPAC010000739.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.guided.444"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            <1..474
                     /locus_tag="ACHUPM_005106"
     CDS             <1..474
                     /locus_tag="ACHUPM_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0872572.1"
                     /translation="DDKFITTDYLQQCPTDREGLDLLRMKVEEGDVILVKKLDRLGRD
                     TADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGR
                     QEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
     gene            657..1517
                     /locus_tag="ACHUPM_005107"
     CDS             657..1517
                     /locus_tag="ACHUPM_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR0872573.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          455 a          374 c          437 g          438 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc caacagaccg ggaagggctg
       61 gatttgctga ggatgaaggt ggaggaaggt gatgtcattc tggttaagaa gctcgaccgt
      121 cttggccgcg acactgccga tatgatccaa ctgataaagg aatttgacgc tcagggcgtg
      181 gcagtccggt tcattgatga cgggatcagt accgacggtg atatggggca aatggtggtc
      241 accatcctgt cggctgtggc acaggctgaa cgccggagga tcctagaacg cacgaatgag
      301 ggccgacagg aagcaaagct gaaaggaatc aaatttggcc gcaggcgtac cgtggacagg
      361 aacgtcgtgc tgacgcttca tcagaagggc actggtgcaa cggaaattgc tcatcagctc
      421 agtattgccc gctccacggt ttataaaatt cttgaagacg aaagggcctc gtgatacgcc
      481 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      601 cgctcatgag acaataaccc tggtaaatgc ttcaataata ttgaaaaagg aagagtatga
      661 gtattcaaca ttttcgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggtgcggta ttatcccgtg
      901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1021 gtgctgccat aaccatgagt gataacactg ctgccaactt acttctgaca acgatcggag
     1081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1201 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1261 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1321 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1381 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1441 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1501 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1561 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1621 aaatccctta acgtgagttt tcgttccact gagcgtcaga cccctgagag cagagatagc
     1681 gctgatgtcc ggcggtgctt ttgc
//
LOCUS       DAZPAC010000740         1302 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.guided.481, whole genome shotgun sequence.
ACCESSION   DAZPAC010000740 DAZPAC010000000
VERSION     DAZPAC010000740.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.guided.481"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            complement(<1..648)
                     /gene="intI1"
                     /locus_tag="ACHUPM_005108"
     CDS             complement(<1..648)
                     /gene="intI1"
                     /locus_tag="ACHUPM_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR0872574.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDAL"
     gene            806..1279
                     /gene="dfrA17"
                     /locus_tag="ACHUPM_005109"
     CDS             806..1279
                     /gene="dfrA17"
                     /locus_tag="ACHUPM_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR0872575.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
BASE COUNT          345 a          327 c          325 g          305 t
ORIGIN      
        1 aagggcgtcg ggaagcgcaa cgccgctgcg gccctcggcc tggtccttca gccaccatgc
       61 ccgtgcacgc gacagctgct cgcgcaggct gggtgccaag ctctcgggta acatcaaggc
      121 ccgatccttg gagcccttgc cctcccgcac gatgatcgtg ccgtgatcga aatccagatc
      181 cttgacccgc agttgcaaac cctcactgat ccgcatgccc gttccataca gaagctgggc
      241 gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga accacttcat ccggggtcag
      301 caccaccggc aagcgccgcg acggccgagg tcttccgatc tcctgaagcc agggcagatc
      361 cgtgcacagc accttgccgt agaagaacag caaggccgcc aatgcctgac gatgcgtgga
      421 gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat gcctcgactt cgctgctgcc
      481 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
      541 agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
      601 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
      661 ctgttttttt gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      721 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      781 aaagttagcc attaagggag ttaaattgaa aatatcattg atttctgcag tgtcagaaaa
      841 tggcgtaatc ggtagtggtc ctgatatccc gtggtcagta aaaggtgagc aactactctt
      901 taaagcgctc acatataatc aatggctcct tgtcggaaga aaaacatttg actctatggg
      961 tgttcttcca aatcgcaaat atgcagtagt gtcaaagaac ggaatttcaa gctcaaatga
     1021 aaacgtccta gtttttcctt caatagaaaa tgctttgaaa gagctatcaa aagttacaga
     1081 tcatgtatat gtctctggcg ggggtcaaat ctataatagc cttattgaaa aagcagatat
     1141 aattcatttg tctactgttc acgttgaagt cgaaggtgat atcaaattcc ctataatgcc
     1201 tgagaatttc aatttggttt ttgaacagtt ttttatgtct aatataaatt atacatacca
     1261 gatttggaaa aaaggctaac aatgcgttgc agcaccagtc gc
//
LOCUS       DAZPAC010000741         1068 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.guided.521, whole genome shotgun sequence.
ACCESSION   DAZPAC010000741 DAZPAC010000000
VERSION     DAZPAC010000741.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.guided.521"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            110..1015
                     /locus_tag="ACHUPM_005110"
     CDS             110..1015
                     /locus_tag="ACHUPM_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR0872576.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT          189 a          343 c          336 g          200 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttc
//
LOCUS       DAZPAC010000742         1068 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2009482
            SAMN43277588-rid23486303.guided.522, whole genome shotgun sequence.
ACCESSION   DAZPAC010000742 DAZPAC010000000
VERSION     DAZPAC010000742.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277588
            Sequence Read Archive: SRR30464314
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396665.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396665.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:14:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,111
            CDSs (total)                      :: 5,020
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 156 of 353
            Pseudo Genes (incomplete)         :: 145 of 353
            Pseudo Genes (internal stop)      :: 109 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277588-rid23486303.guided.522"
                     /strain="M2009482"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-12-17"
                     /collected_by="FSS"
     gene            110..1015
                     /locus_tag="ACHUPM_005111"
     CDS             110..1015
                     /locus_tag="ACHUPM_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR0872577.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFAPALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT          188 a          344 c          336 g          200 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgcccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttc
//
